LOCUS       DAHBHC010000001       138525 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHBHC010000001 DAHBHC010000000
VERSION     DAHBHC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 138525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 138525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.001"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="M2A35_000001"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="M2A35_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCB7196353.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="M2A35_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="M2A35_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCB7196354.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="M2A35_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="M2A35_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCB7196355.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="M2A35_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="M2A35_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCB7196356.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="M2A35_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="M2A35_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCB7196357.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="M2A35_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="M2A35_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCB7196358.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="M2A35_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="M2A35_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCB7196359.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="M2A35_000008"
     CDS             complement(4598..5320)
                     /locus_tag="M2A35_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7196360.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="M2A35_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="M2A35_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCB7196361.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="M2A35_000010"
     CDS             6520..6738
                     /locus_tag="M2A35_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCB7196362.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="M2A35_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="M2A35_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCB7196363.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="M2A35_000012"
     CDS             complement(7472..7672)
                     /locus_tag="M2A35_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCB7196364.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="M2A35_000013"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="M2A35_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCB7196365.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="M2A35_000014"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="M2A35_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCB7196366.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="M2A35_000015"
     CDS             10169..12082
                     /locus_tag="M2A35_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7196367.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="M2A35_000016"
     CDS             12082..13104
                     /locus_tag="M2A35_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCB7196368.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="M2A35_000017"
     CDS             13098..13316
                     /locus_tag="M2A35_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCB7196369.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="M2A35_000018"
     CDS             13370..14239
                     /locus_tag="M2A35_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCB7196370.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="M2A35_000019"
     CDS             complement(14294..14698)
                     /locus_tag="M2A35_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCB7196371.1"
                     /translation="MQARVKWVEGLIFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="M2A35_000020"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="M2A35_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCB7196372.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="M2A35_000021"
     CDS             15683..17773
                     /locus_tag="M2A35_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCB7196373.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="M2A35_000022"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="M2A35_000022"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCB7196374.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="M2A35_000023"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="M2A35_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCB7196375.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /locus_tag="M2A35_000024"
     CDS             complement(19741..20343)
                     /locus_tag="M2A35_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCB7196376.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="M2A35_000025"
     CDS             complement(20333..20500)
                     /locus_tag="M2A35_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCB7196377.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="M2A35_000026"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="M2A35_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCB7196378.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="M2A35_000027"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="M2A35_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCB7196379.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="M2A35_000028"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="M2A35_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCB7196380.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="M2A35_000029"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="M2A35_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCB7196381.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="M2A35_000030"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="M2A35_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCB7196382.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cobA"
                     /locus_tag="M2A35_000031"
     CDS             26709..28082
                     /gene="cobA"
                     /locus_tag="M2A35_000031"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCB7196383.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="M2A35_000032"
     CDS             28328..28495
                     /locus_tag="M2A35_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCB7196384.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28739..30127
                     /gene="frlA"
                     /locus_tag="M2A35_000033"
     CDS             28739..30127
                     /gene="frlA"
                     /locus_tag="M2A35_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCB7196385.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGAQGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="M2A35_000034"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="M2A35_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCB7196386.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQHGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32050
                     /gene="frlC"
                     /locus_tag="M2A35_000035"
     CDS             31220..32050
                     /gene="frlC"
                     /locus_tag="M2A35_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCB7196387.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32047..32832
                     /gene="frlD"
                     /locus_tag="M2A35_000036"
     CDS             32047..32832
                     /gene="frlD"
                     /locus_tag="M2A35_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCB7196388.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32932..33663
                     /gene="phoB"
                     /locus_tag="M2A35_000037"
     CDS             32932..33663
                     /gene="phoB"
                     /locus_tag="M2A35_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCB7196389.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33815..34900)
                     /locus_tag="M2A35_000038"
     CDS             complement(33815..34900)
                     /locus_tag="M2A35_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCB7196390.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANISTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQNQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34912..36216)
                     /locus_tag="M2A35_000039"
     CDS             complement(34912..36216)
                     /locus_tag="M2A35_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCB7196391.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36228..36581)
                     /locus_tag="M2A35_000040"
     CDS             complement(36228..36581)
                     /locus_tag="M2A35_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCB7196392.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36592..37470)
                     /locus_tag="M2A35_000041"
     CDS             complement(36592..37470)
                     /locus_tag="M2A35_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCB7196393.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37467..38693)
                     /locus_tag="M2A35_000042"
     CDS             complement(37467..38693)
                     /locus_tag="M2A35_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCB7196394.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38693..39856)
                     /locus_tag="M2A35_000043"
     CDS             complement(38693..39856)
                     /locus_tag="M2A35_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCB7196395.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39940..40302)
                     /locus_tag="M2A35_000044"
     CDS             complement(39940..40302)
                     /locus_tag="M2A35_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCB7196396.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40319..41224)
                     /locus_tag="M2A35_000045"
     CDS             complement(40319..41224)
                     /locus_tag="M2A35_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196397.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41493..42497)
                     /gene="trpS"
                     /locus_tag="M2A35_000046"
     CDS             complement(41493..42497)
                     /gene="trpS"
                     /locus_tag="M2A35_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCB7196398.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42490..43248)
                     /gene="gph"
                     /locus_tag="M2A35_000047"
     CDS             complement(42490..43248)
                     /gene="gph"
                     /locus_tag="M2A35_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCB7196399.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43241..43918)
                     /gene="rpe"
                     /locus_tag="M2A35_000048"
     CDS             complement(43241..43918)
                     /gene="rpe"
                     /locus_tag="M2A35_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCB7196400.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43936..44772)
                     /gene="dam"
                     /locus_tag="M2A35_000049"
     CDS             complement(43936..44772)
                     /gene="dam"
                     /locus_tag="M2A35_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCB7196401.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44879..46165)
                     /gene="damX"
                     /locus_tag="M2A35_000050"
     CDS             complement(44879..46165)
                     /gene="damX"
                     /locus_tag="M2A35_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCB7196402.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46257..47345)
                     /gene="aroB"
                     /locus_tag="M2A35_000051"
     CDS             complement(46257..47345)
                     /gene="aroB"
                     /locus_tag="M2A35_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCB7196403.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47402..47923)
                     /gene="aroK"
                     /locus_tag="M2A35_000052"
     CDS             complement(47402..47923)
                     /gene="aroK"
                     /locus_tag="M2A35_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCB7196404.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48324..49562)
                     /gene="hofQ"
                     /locus_tag="M2A35_000053"
     CDS             complement(48324..49562)
                     /gene="hofQ"
                     /locus_tag="M2A35_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCB7196405.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49474..49878)
                     /gene="hofP"
                     /locus_tag="M2A35_000054"
     CDS             complement(49474..49878)
                     /gene="hofP"
                     /locus_tag="M2A35_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCB7196406.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49868..50308)
                     /gene="hofO"
                     /locus_tag="M2A35_000055"
     CDS             complement(49868..50308)
                     /gene="hofO"
                     /locus_tag="M2A35_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCB7196407.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50292..50831)
                     /gene="hofN"
                     /locus_tag="M2A35_000056"
     CDS             complement(50292..50831)
                     /gene="hofN"
                     /locus_tag="M2A35_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCB7196408.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50831..51610)
                     /gene="hofM"
                     /locus_tag="M2A35_000057"
     CDS             complement(50831..51610)
                     /gene="hofM"
                     /locus_tag="M2A35_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCB7196409.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            51730..54282
                     /gene="mrcA"
                     /locus_tag="M2A35_000058"
     CDS             51730..54282
                     /gene="mrcA"
                     /locus_tag="M2A35_000058"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCB7196410.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54447..55007)
                     /gene="nudE"
                     /locus_tag="M2A35_000059"
     CDS             complement(54447..55007)
                     /gene="nudE"
                     /locus_tag="M2A35_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCB7196411.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55327..57462
                     /gene="igaA"
                     /locus_tag="M2A35_000060"
     CDS             55327..57462
                     /gene="igaA"
                     /locus_tag="M2A35_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCB7196412.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57527..58195
                     /gene="yrfG"
                     /locus_tag="M2A35_000061"
     CDS             57527..58195
                     /gene="yrfG"
                     /locus_tag="M2A35_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCB7196413.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58206..58607
                     /gene="hslR"
                     /locus_tag="M2A35_000062"
     CDS             58206..58607
                     /gene="hslR"
                     /locus_tag="M2A35_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCB7196414.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58632..59510
                     /gene="hslO"
                     /locus_tag="M2A35_000063"
     CDS             58632..59510
                     /gene="hslO"
                     /locus_tag="M2A35_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCB7196415.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59645..61369)
                     /locus_tag="M2A35_000064"
     CDS             complement(59645..61369)
                     /locus_tag="M2A35_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCB7196416.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61747..63369
                     /gene="pckA"
                     /locus_tag="M2A35_000065"
     CDS             61747..63369
                     /gene="pckA"
                     /locus_tag="M2A35_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCB7196417.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCSNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63445..64797)
                     /gene="envZ"
                     /locus_tag="M2A35_000066"
     CDS             complement(63445..64797)
                     /gene="envZ"
                     /locus_tag="M2A35_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCB7196418.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64794..65513)
                     /gene="ompR"
                     /locus_tag="M2A35_000067"
     CDS             complement(64794..65513)
                     /gene="ompR"
                     /locus_tag="M2A35_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCB7196419.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65741..66217
                     /gene="greB"
                     /locus_tag="M2A35_000068"
     CDS             65741..66217
                     /gene="greB"
                     /locus_tag="M2A35_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCB7196420.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66314..68635
                     /locus_tag="M2A35_000069"
     CDS             66314..68635
                     /locus_tag="M2A35_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCB7196421.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69073..69300
                     /gene="feoA"
                     /locus_tag="M2A35_000070"
     CDS             69073..69300
                     /gene="feoA"
                     /locus_tag="M2A35_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCB7196422.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69317..71638
                     /gene="feoB"
                     /locus_tag="M2A35_000071"
     CDS             69317..71638
                     /gene="feoB"
                     /locus_tag="M2A35_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCB7196423.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKPVSSCCAASTTGDCH"
     gene            71638..71874
                     /gene="feoC"
                     /locus_tag="M2A35_000072"
     CDS             71638..71874
                     /gene="feoC"
                     /locus_tag="M2A35_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCB7196424.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72077..72955
                     /gene="rpnA"
                     /locus_tag="M2A35_000073"
     CDS             72077..72955
                     /gene="rpnA"
                     /locus_tag="M2A35_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCB7196425.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(72984..73754)
                     /gene="bioH"
                     /locus_tag="M2A35_000074"
     CDS             complement(72984..73754)
                     /gene="bioH"
                     /locus_tag="M2A35_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCB7196426.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73792..74475
                     /gene="gntX"
                     /locus_tag="M2A35_000075"
     CDS             73792..74475
                     /gene="gntX"
                     /locus_tag="M2A35_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCB7196427.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74534..75109
                     /gene="nfuA"
                     /locus_tag="M2A35_000076"
     CDS             74534..75109
                     /gene="nfuA"
                     /locus_tag="M2A35_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCB7196428.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75470..76786
                     /gene="gntT"
                     /locus_tag="M2A35_000077"
     CDS             75470..76786
                     /gene="gntT"
                     /locus_tag="M2A35_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCB7196429.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76831..78915)
                     /gene="malQ"
                     /locus_tag="M2A35_000078"
     CDS             complement(76831..78915)
                     /gene="malQ"
                     /locus_tag="M2A35_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCB7196430.1"
                     /translation="MESKRLDNAALAAGISPNYINAYGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDHE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(78925..81318)
                     /gene="malP"
                     /locus_tag="M2A35_000079"
     CDS             complement(78925..81318)
                     /gene="malP"
                     /locus_tag="M2A35_000079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCB7196431.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTEVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            81930..84635
                     /gene="malT"
                     /locus_tag="M2A35_000080"
     CDS             81930..84635
                     /gene="malT"
                     /locus_tag="M2A35_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCB7196432.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWATGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84678..85694)
                     /gene="rtcA"
                     /locus_tag="M2A35_000081"
     CDS             complement(84678..85694)
                     /gene="rtcA"
                     /locus_tag="M2A35_000081"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCB7196433.1"
                     /translation="MKRMIALDGAQGEGGGQSLRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGIVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85698..86924)
                     /gene="rtcB"
                     /locus_tag="M2A35_000082"
     CDS             complement(85698..86924)
                     /gene="rtcB"
                     /locus_tag="M2A35_000082"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCB7196434.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAEMRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87113..88711
                     /gene="rtcR"
                     /locus_tag="M2A35_000083"
     CDS             87113..88711
                     /gene="rtcR"
                     /locus_tag="M2A35_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCB7196435.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(88693..89451)
                     /locus_tag="M2A35_000084"
     CDS             complement(88693..89451)
                     /locus_tag="M2A35_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCB7196436.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89468..90298)
                     /gene="glpG"
                     /locus_tag="M2A35_000085"
     CDS             complement(89468..90298)
                     /gene="glpG"
                     /locus_tag="M2A35_000085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCB7196437.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90343..90669)
                     /gene="glpE"
                     /locus_tag="M2A35_000086"
     CDS             complement(90343..90669)
                     /gene="glpE"
                     /locus_tag="M2A35_000086"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCB7196438.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            90859..92364
                     /gene="glpD"
                     /locus_tag="M2A35_000087"
     CDS             90859..92364
                     /gene="glpD"
                     /locus_tag="M2A35_000087"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCB7196439.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92416..93021)
                     /locus_tag="M2A35_000088"
     CDS             complement(92416..93021)
                     /locus_tag="M2A35_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196440.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(93024..93791)
                     /locus_tag="M2A35_000089"
     CDS             complement(93024..93791)
                     /locus_tag="M2A35_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196441.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(93794..94504)
                     /locus_tag="M2A35_000090"
     CDS             complement(93794..94504)
                     /locus_tag="M2A35_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196442.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(94520..96028)
                     /locus_tag="M2A35_000091"
     CDS             complement(94520..96028)
                     /locus_tag="M2A35_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCB7196443.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96153..98600)
                     /gene="glgP"
                     /locus_tag="M2A35_000092"
     CDS             complement(96153..98600)
                     /gene="glgP"
                     /locus_tag="M2A35_000092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCB7196444.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(98619..100052)
                     /gene="glgA"
                     /locus_tag="M2A35_000093"
     CDS             complement(98619..100052)
                     /gene="glgA"
                     /locus_tag="M2A35_000093"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCB7196445.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(100052..101347)
                     /gene="glgC"
                     /locus_tag="M2A35_000094"
     CDS             complement(100052..101347)
                     /gene="glgC"
                     /locus_tag="M2A35_000094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCB7196446.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101365..103338)
                     /gene="glgX"
                     /locus_tag="M2A35_000095"
     CDS             complement(101365..103338)
                     /gene="glgX"
                     /locus_tag="M2A35_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCB7196447.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103335..105521)
                     /gene="glgB"
                     /locus_tag="M2A35_000096"
     CDS             complement(103335..105521)
                     /gene="glgB"
                     /locus_tag="M2A35_000096"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCB7196448.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVYSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(105794..106897)
                     /gene="asd"
                     /locus_tag="M2A35_000097"
     CDS             complement(105794..106897)
                     /gene="asd"
                     /locus_tag="M2A35_000097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCB7196449.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            107090..107683
                     /gene="yhgN"
                     /locus_tag="M2A35_000098"
     CDS             107090..107683
                     /gene="yhgN"
                     /locus_tag="M2A35_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCB7196450.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(107729..109024)
                     /locus_tag="M2A35_000099"
     CDS             complement(107729..109024)
                     /locus_tag="M2A35_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCB7196451.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHENDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(108931..111027)
                     /locus_tag="M2A35_000100"
     CDS             complement(108931..111027)
                     /locus_tag="M2A35_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HCB7196452.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTPRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(111167..112507)
                     /gene="gntU"
                     /locus_tag="M2A35_000101"
     CDS             complement(111167..112507)
                     /gene="gntU"
                     /locus_tag="M2A35_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCB7196453.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(112511..113038)
                     /gene="gntK"
                     /locus_tag="M2A35_000102"
     CDS             complement(112511..113038)
                     /gene="gntK"
                     /locus_tag="M2A35_000102"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCB7196454.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLKALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(113177..114172)
                     /gene="gntR"
                     /locus_tag="M2A35_000103"
     CDS             complement(113177..114172)
                     /gene="gntR"
                     /locus_tag="M2A35_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCB7196455.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(114396..115091)
                     /gene="yhhW"
                     /locus_tag="M2A35_000104"
     CDS             complement(114396..115091)
                     /gene="yhhW"
                     /locus_tag="M2A35_000104"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCB7196456.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(115214..116251)
                     /locus_tag="M2A35_000105"
     CDS             complement(115214..116251)
                     /locus_tag="M2A35_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCB7196457.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRHHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            116583..117071
                     /gene="yhhY"
                     /locus_tag="M2A35_000106"
     CDS             116583..117071
                     /gene="yhhY"
                     /locus_tag="M2A35_000106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7196458.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            117308..118486
                     /locus_tag="M2A35_000107"
     CDS             117308..118486
                     /locus_tag="M2A35_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCB7196459.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNIMDHTK
                     "
     gene            118483..118977
                     /locus_tag="M2A35_000108"
     CDS             118483..118977
                     /locus_tag="M2A35_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196460.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            119072..119227
                     /locus_tag="M2A35_000109"
     CDS             119072..119227
                     /locus_tag="M2A35_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196461.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            119427..119711
                     /locus_tag="M2A35_000110"
     CDS             119427..119711
                     /locus_tag="M2A35_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HCB7196462.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(119749..121491)
                     /gene="ggt"
                     /locus_tag="M2A35_000111"
     CDS             complement(119749..121491)
                     /gene="ggt"
                     /locus_tag="M2A35_000111"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCB7196463.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            121611..122051
                     /locus_tag="M2A35_000112"
     CDS             121611..122051
                     /locus_tag="M2A35_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCB7196464.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(122038..122781)
                     /gene="ugpQ"
                     /locus_tag="M2A35_000113"
     CDS             complement(122038..122781)
                     /gene="ugpQ"
                     /locus_tag="M2A35_000113"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCB7196465.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(122778..123848)
                     /gene="ugpC"
                     /locus_tag="M2A35_000114"
     CDS             complement(122778..123848)
                     /gene="ugpC"
                     /locus_tag="M2A35_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCB7196466.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKCRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(123850..124695)
                     /gene="ugpE"
                     /locus_tag="M2A35_000115"
     CDS             complement(123850..124695)
                     /gene="ugpE"
                     /locus_tag="M2A35_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCB7196467.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASASATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(124692..125579)
                     /gene="ugpA"
                     /locus_tag="M2A35_000116"
     CDS             complement(124692..125579)
                     /gene="ugpA"
                     /locus_tag="M2A35_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCB7196468.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(125677..126993)
                     /gene="ugpB"
                     /locus_tag="M2A35_000117"
     CDS             complement(125677..126993)
                     /gene="ugpB"
                     /locus_tag="M2A35_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCB7196469.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(127389..128102)
                     /gene="livF"
                     /locus_tag="M2A35_000118"
     CDS             complement(127389..128102)
                     /gene="livF"
                     /locus_tag="M2A35_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCB7196470.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(128104..128871)
                     /gene="livG"
                     /locus_tag="M2A35_000119"
     CDS             complement(128104..128871)
                     /gene="livG"
                     /locus_tag="M2A35_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCB7196471.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(128868..130145)
                     /gene="livM"
                     /locus_tag="M2A35_000120"
     CDS             complement(128868..130145)
                     /gene="livM"
                     /locus_tag="M2A35_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCB7196472.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(130142..131068)
                     /gene="livH"
                     /locus_tag="M2A35_000121"
     CDS             complement(130142..131068)
                     /gene="livH"
                     /locus_tag="M2A35_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCB7196473.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(131116..132225)
                     /gene="livK"
                     /locus_tag="M2A35_000122"
     CDS             complement(131116..132225)
                     /gene="livK"
                     /locus_tag="M2A35_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCB7196474.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            132649..133032
                     /gene="panM"
                     /locus_tag="M2A35_000123"
     CDS             132649..133032
                     /gene="panM"
                     /locus_tag="M2A35_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCB7196475.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(133231..134247)
                     /locus_tag="M2A35_000124"
     CDS             complement(133231..134247)
                     /locus_tag="M2A35_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HCB7196476.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMITPAATAPELTA
                     RGYQLILRTTGLDSDQGPTAAKYILEKVKPQRIAIVHDKQQYGEGLARAVQDGLKKGN
                     ANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAGLKTQFMG
                     PEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFVWTTYA
                     ALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLKGFEFGVFDWHANGTA
                     TDAK"
     gene            complement(134518..135372)
                     /gene="rpoH"
                     /locus_tag="M2A35_000125"
     CDS             complement(134518..135372)
                     /gene="rpoH"
                     /locus_tag="M2A35_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCB7196477.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(135617..136675)
                     /gene="ftsX"
                     /locus_tag="M2A35_000126"
     CDS             complement(135617..136675)
                     /gene="ftsX"
                     /locus_tag="M2A35_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCB7196478.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(136668..137336)
                     /gene="ftsE"
                     /locus_tag="M2A35_000127"
     CDS             complement(136668..137336)
                     /gene="ftsE"
                     /locus_tag="M2A35_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCB7196479.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(137339..>138525)
                     /gene="ftsY"
                     /locus_tag="M2A35_000128"
     CDS             complement(137339..>138525)
                     /gene="ftsY"
                     /locus_tag="M2A35_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCB7196480.1"
                     /translation="PVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAK
                     EEITDEELEAQALAAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLK
                     TKENLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRD
                     AEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQG
                     KSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVL
                     IADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEA
                     VGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFAR
                     ED"
BASE COUNT        33624 a        37636 c        34578 g        32687 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccacca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaaattaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg caggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcaggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca taaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaacat accccttaag
    22801 aagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtacag ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggta gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaacttgaa tcactgctgc cgttgcatct gggccaggta gcgaaatacg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgggtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgatcgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaaccacc gaacagttaa tcaacgaacc gcttgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggcagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatatccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacata acatcatatg ttgttatatt cattatgcat tgtcatgtta ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccctac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctggatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgcagg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctggaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacacca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggatcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gtcgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagcat ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccgaat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggctatacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgcgaa agcctcgaca tgatcaagct
    31501 ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttccg cggcccacgc
    31561 gggctatctc acgccaccta atgttatctg gggacggctg gcagagaacc taagcgaact
    31621 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31681 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31741 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31801 ttatttcgac aaactgggtg ataaattgcg tcatctgcat attgtcgaca gcgacggggc
    31861 cagtgacaca cattacattc ccggcgaagg caaaatgccg ctgcgggagc tgatgcgcga
    31921 tattatcgac cggggctatg acggttactg cacggtggaa ctggtgacga tgtatatgaa
    31981 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    32041 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32101 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32161 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32221 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32281 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32341 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32401 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32461 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32521 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32581 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32641 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32701 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32761 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32821 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32881 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32941 acggaccgct actctcatca actcctctac gctaccgtcc gccagcgact gctggatgat
    33001 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33061 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33121 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33181 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag
    33241 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33301 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33361 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33421 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33481 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33541 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33601 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa ctattgataa taaaagacat
    33661 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcgttgaatt
    33721 ttacatcccg tacgttcctc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33781 agtacggaat ttagcttgac ggtgctatcc agcattaaat actggcaata ctctcacgca
    33841 aaatccgcag caccgtctct tcaccgctgc gattcgggtt aatgcgaatc gcacaatgtt
    33901 ctgattgtgg attcgcctgg cgaaacgttc cggaaaggcg ataaaagagc ggcgggattt
    33961 catatttcga ctctgcaccc accgggtaag gcaaggcacc gcgcttttgc gcctcttcca
    34021 gcactctggc ggcaatcggc tgatgaaact cgacaatcaa caccttcgac tgcgcattag
    34081 caatcaccgc gcttttcact tccggcaccg caccaccgtt aagcaaagcc agcaaccgtt
    34141 cagatacccc tgcctgcacc gcgtgcatca ctggcgcaaa caccagacca cgcaagactt
    34201 ccagcgcctg tgcgccctgg ttctggctac cgccggagta aagcgtggcg cgaatacggt
    34261 tgataacatc agcatcgccg accactgcac caacaccctc tggcccaaat agcttgaagc
    34321 aggaaaatgt cgagatattc gcgccgcatt cacagccgat tcgcgccacc ttcatcaccg
    34381 catagttgtc atcggttaac gctggaacac ctgccgcgcg caacgttgcc agcacatctg
    34441 ccagcacgta gctgtcctgc ggctgctggc gcgtatgctg caccagcgcc gcatccggtt
    34501 gttgctcgtc gacgacctgc ttcagtgccg acaggtcatt gaaatcaaca gtaataagcg
    34561 tcagccccat ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg tcatgcacca
    34621 gaagacgctg ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg ccagtccccg
    34681 cgccctgcac cagcgccgca gcctgtgcgt gaaatgcatc agccagcacc tgttccacac
    34741 gctgggtaac gcgcggttga ttcagtcctg gcgttaagcc taaatcaccg ccagtaagaa
    34801 actcgctgcc ggggaaatgg cgacaaatgc tatccaccag cgcaaactgc ttttgctgcg
    34861 cctcaataat cgtcaggctt tgcagaggaa acgtcttcat aggtgccttc cttaagccgg
    34921 aacgaacagg ccaagccagt aaagaacatt cagcagaata ccggtgatca ttactgccac
    34981 gaccggtgcc gccattttct gtaccggacg gcccagggat tcgttgagga agtaaatggc
    35041 aaccgcgata gagaatccgg tataacccgc catcttaatt gctgcgaaaa tcgaaccgac
    35101 cagcagcgcc acttccatca gcatattcat ggcgttacgg atgttatccg acgcattacg
    35161 caccgacggg tagcgtccca gccatttgcc gatcgaacga agcagcaaga cttccgccga
    35221 aataaccact gcgcctaata ccgctgcaac catcggattc ggcgagagat agcccaccgc
    35281 ataaacaaag gtaaagcccg caactgcata cacacccgtt gctaaggcgg tggtggcaat
    35341 caacggtaca aaacccagcc cgcgcataaa ttctgccagt gccgcctggt tgatcagcgt
    35401 ttgcgattgt tccggcgtta cgcctgcgga gtacgctttc tccagagtga agatcgacac
    35461 ttcactgccg gcgaaaatct tcatgctggc aacggcggca atcaatgctc ccacgatggc
    35521 gatatagggt aagtttttga taatccgcga cgtgcgttct tcaaacaccg aaagcccgct
    35581 ggcatcaata tcattttcat cacgatgacg caggtcgtga gttatcgcga tccccagcag
    35641 catcaccatg ccaataaaga tttcgatgga ttcagggtta agatgtggga aatagcgcac
    35701 gactaccaca cgggtcatca gaacaaccac ggcggcgacc agactttgct tccagccaaa
    35761 ctggtagaaa atcgccacca gcgggaacag tgcaaaagca gaaaccaccg gcgagcttaa
    35821 ttccccgagg ctacctaata catccaccgg cagcgcggtc agcagctggt ttactggcaa
    35881 caggcaagta aggatcaata cgccccagat agcgccaaga ccaaacgcca tcaggctgtt
    35941 tatcgccagt acgccgagga tatcggtagg aagaaagagt aaccatgcgt tgagcagtcc
    36001 ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca gaccaaaagc
    36061 gatactcccc gcctcgcgac ggttcatata gccttcaatc agttgcggca ggatcgggcg
    36121 gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca tacccgtcag
    36181 gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta tttcagatgg
    36241 ttaatcagca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa cgccactttt
    36301 ccttcagcga tcattttggc gatatgctcg tctttcgctt taatgcctgg tttggcaatg
    36361 gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc gcccgcaccg
    36421 gtattacacg caccgatgta gtaatccagt tgcccggatt tcactttcat tgccgcttcc
    36481 atgtcgttgt gaatgaaaac ttcaaaactg ccaggagccg tcgcttcaat ggttttttta
    36541 atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc cttattggaa
    36601 aaattgagag ggagtttcac gtaacatcac atccacatcg gcctgactga atcctgactg
    36661 gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac cattggcttt
    36721 taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca acccgcggtc
    36781 acgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt tcttaccaat
    36841 ggtgtcgaac tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt cgaggttgtc
    36901 tttcagatcg cagtgaccaa cggtgacgcg cgaaagatca actccgtggg cttgcaacag
    36961 agccagttgt tccagcccca tggtgctgaa cgaagtatgc gtggagatcg ggcgtccggt
    37021 ctggttatgc gccagcgcag cggcaataaa caccttctct tccagcggcg taatcttccc
    37081 ttcgctagag ccgatctccg cgataatccc ggctttcagg tcggtgccat cgataccctg
    37141 ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtcg ccacatgctc
    37201 cgggaaaaaa gcgtcctggt aataaccggt gcaggccacc acgttgatcc ccgtctcgcg
    37261 cattacatcg agcataaatt gcgcattgcg ccccatgtaa cggttggtca tctcaatcac
    37321 attacgcacg ccccgggtca tcaggtcgtt catctcctgg caaatgaacg catactgatc
    37381 aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat gctcatgggc
    37441 cagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg gagggaggaa
    37501 agaaaagagc gaccattttg tggcggtggc gcacggaaaa attcacacac ggtagccccc
    37561 acatctgaaa gcgtggtgcg cacaccgagc tgcgtagcta tcatcccttg ctgataaacc
    37621 agcactggca ccacttcgcg ggtatggtgg ctgtgaccaa tggtcggatc gttaccgtga
    37681 tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg ggcaaggtta
    37741 cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg accagcgagg
    37801 tcggtttcct ggatattggt gcaaataaac gccgtcggat gcgtgttaaa ttcgtcaagg
    37861 gtgatatcca taatccgctg gctatccacc aggttttgcc agctcacgcc ataaggattg
    37921 ctgacgatat ctgccacttt acctaccagc acggttggca cgcccgcctc atacagtttt
    37981 tgcggcacct gcactttttc atcaacaccg tagcccatat gcacgacctg gaaaccgttg
    38041 tcataagcgc cagaacgcgg cgcattgatg ccaataaagc gcccttcttt gctttctgcg
    38101 gcatcgagaa tacgctgact gtcggttaac aagccgccaa atgtaatgac ccgaccgacc
    38161 tgtacctgct cacgcacgat acgaccaatt ttgattgcgt cgtcaaaaga gatcacagag
    38221 agattggcgg taatgttata gacctggcct aaatccgcct cgagattatc gccaatcgca
    38281 accgcctgat tgacccacag aaattgcaga tcatcgccac ggcgctccac ctgccagcca
    38341 gcggaaacta atgcctgctc aacacggtca atcacatcgc gaaaaggcat ccgcagcggc
    38401 ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc accttcatgt
    38461 tgcagctctg ccacgcccca ggttgcggaa tctgacggct gcatatcgcc tggcgcataa
    38521 cccaatgcgt tgattagccc cagcttctcc agcgttggta gctgcaaatg cggcaactgg
    38581 ctcaggatgt gaccacaggt attcgctccc gcatcttgcg gacgcaccag cgtgacatct
    38641 ttcattgccc ctacgccaaa gctatcaatc actaacacca caaatcgcgc cattacgccc
    38701 ccagagagtt tccaagactg tcataacgac caacgatttt cggttcgcca cggtgaatac
    38761 cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcggaaa cagagcacca
    38821 ctgcactgct taccggaaac tcgcctgcca gcgacagggt gtagtcgata ctgctgtcat
    38881 ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac accaacgcat
    38941 gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat
    39001 gatgggagat ttcactcaac cagagcatcg cgatacgttc agactgatcg ccctgctggt
    39061 ttgccggaat agtgcccgtc agcgcatgac cgggttcggc atgagtcaca ccgtattgcg
    39121 ccagtaatgg cagcgaagtg cagctggtcg ctgaaggcgc gttcagttgc tcaatcgcaa
    39181 taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgtcggca
    39241 aaactttccc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt ccggctaaat
    39301 gcagccccgg cagtttctta atttcagcaa ccacctcgtg cagcgaatgc tgtacaaaac
    39361 cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc agcacagact
    39421 ggattcgccc ggccttcacc gccgccgcag aaacttcccg cgctttgtcg agagtaaaca
    39481 cggtgatgac gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa gggatttgta
    39541 ccagatgccc ctgatgcgcc accggcaaac cagcgcggcg catgactcgc gcctctttgt
    39601 aatccaccgc cacaatgccg ctgtagccca acgccagcag tttttccgcc agccatgggt
    39661 tacgaccgaa ttgtttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa
    39721 tcagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg tccggggcga
    39781 ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc tggcgtttca
    39841 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    39901 tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt cgttagcagc
    39961 catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt taacttccag
    40021 tgcgaattcc ttcaacaata cctgatgcaa ttgcacaaca gcttgccagt ggctggattg
    40081 cgccagctct gccagcaatt cgttatccag cggctctatt tcttcaccac ggcgactgcg
    40141 catcagtgca ctcgccatat gtgtaatcgc catcgttcct tgctcactgc gcactggcag
    40201 atggcactct gtttccagta cgttgacgac ctgcatcatg cctttgcaga tgtccttatc
    40261 aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    40321 aataactcgg ttcctgttcc ccactcacca cgcgttgttg atgggcgagc agggcgtgtt
    40381 tatctacaac ggcacgcagc agttgttgca ttgggtaatc atcggctcgc gtcaggacca
    40441 cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgcc tctaatccca
    40501 gcatggtcag ttcgttttcc gccaccacgt tccagataac cgcatcgaca tcgcctttaa
    40561 caatgcgttg taaactctcg tgataagaga gatcgacacg ttccacatca ctatcgccaa
    40621 aaaagacatc ggtcatgatt ttctgatccg ccgaacggtt atccagcccc acgcgcttca
    40681 cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag gtgtgcggcc
    40741 ccaactccag cgcgaggcaa aacccttttt gcgtgagata actttccgcc gccagtcggg
    40801 aaaccaccgc catgtcatac acgccgttaa gtaaacactc cacgcgaata tccgcgccgc
    40861 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgctaaac
    40921 cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg atatccacat
    40981 gactcagcaa tgctttgtta tccatctcga ccagataact gccattgcgc ccacggcgtt
    41041 caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaacgg
    41101 atgaacggca ttcagttgcc agctcatcta tggttttcaa cctattaccg catttttcac
    41161 ccaacaaata acgggccagc gtcgtgataa cgacgccttc ttttttgata aacgttcgac
    41221 gcataatata ttttcaataa aatgaatgtt tatatcttca ttaaaatgaa gataagtggc
    41281 aactgtgaga tgtggaaacg gcgaggcgct tcacattttt tcggattatt gcaaatgagg
    41341 ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat tcgacaatgc
    41401 gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc ctctccccaa
    41461 aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct
    41521 tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc gccgtctttc
    41581 atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc ctgcaattca
    41641 gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata catcttgcct
    41701 tcgaactgtt tttccagttc tggaatgctc tggcccgtta ccgcagaaag gatatccagc
    41761 aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag
    41821 tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag caggccgata
    41881 acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt
    41941 acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc gccatacagc
    42001 gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg gtcttcaccc
    42061 accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca
    42121 aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgggtcatg
    42181 cggctcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttct
    42241 ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac
    42301 aaggccagcg tatccagcgt cgctttacgc agcttctgtg catcctggcg cacggtgatc
    42361 gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga
    42421 cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa
    42481 acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg
    42541 cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc
    42601 gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg ccgcctgaat
    42661 atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca tccgctcagc
    42721 caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc cgccgataac
    42781 cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg
    42841 tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc
    42901 aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc gatcgaacag
    42961 tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttacc
    43021 cattgtttta cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag
    43081 aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc
    43141 cagcgcatac agcgccatat ctaccgcagc agcaagaccg ggagcactgt cgaccagcgt
    43201 accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga cttacctttg
    43261 ccagttcact gcgcatttca tcaatgactt ttttgtagtc tggctggtcg aagattgccg
    43321 aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca
    43381 cgccaccgtc tacttccaga cgaatgtcaa agccagactc gtcgatacga cgacgtactt
    43441 cgcgcagttt atccagtgtt tgaggaatga acgactgacc gccgaaacca ggattgacgg
    43501 acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc agcggtgtcg
    43561 ccgggttaaa taccagtccc gctttacagc cattttcttt aatcagttgc agcgtgcggt
    43621 caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat
    43681 caggcacaat gcgatcgaca ggtttcacca tcaggtgtac gtcgataggg gcggtaatgc
    43741 catagttacg caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt
    43801 tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt
    43861 cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc
    43921 gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag
    43981 cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac
    44041 gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat
    44101 cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg
    44161 ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc
    44221 ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgcgccatgc tatcggcgta
    44281 agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc
    44341 cgggaaatag ggttttttgt agcggccaaa cggcacatta aactcaccgc gcagattgta
    44401 acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa
    44461 cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt
    44521 ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat
    44581 gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt
    44641 gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa
    44701 atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg
    44761 atttttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca
    44821 gcatccagcg cttcaggcaa ctcccgcaac gataaaccat tgcgggagat aaccttgatt
    44881 acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctggacat
    44941 ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa
    45001 ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat
    45061 tctctttctt cgcccaaccg ttcaggttgc cgtagttaga ggaactgctc agctgcagag
    45121 tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag
    45181 cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg
    45241 tctcttttgt tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc acaggggcag
    45301 ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ctccgttttc acggttgctt
    45361 gcggtttttt cggttcaatc actgcctgct ggcgagctgg acgcgtagtg gccggacgtt
    45421 cagcggtttg cgttttcgcc gtttcacggg atgcattgcc attgcgaaca ggcgcgacag
    45481 tcgcgggttc ggtcggcaat gtggaattga ccgccacatt gttcaactgt tgctgatttt
    45541 gtggttgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg
    45601 ttgccgccgg ggtttgccct tgagtcggcg tagaagagat cggcggcaga gaaacatcct
    45661 gctgagtatt ttccgcagac gtggttcccg gcgctggctg cacgccattg gcctgatcgg
    45721 tcgcattgcc agcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagagg
    45781 tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc
    45841 cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac
    45901 gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt
    45961 cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag
    46021 tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag
    46081 aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct
    46141 cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt
    46201 tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac
    46261 gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact
    46321 tcactcttac caattgccaa cggaagaatt aagcgcatct ctcccgcaag gactttcttg
    46381 tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt
    46441 aacccagccc gcgtgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg
    46501 agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac
    46561 caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt
    46621 aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa
    46681 caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc
    46741 tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc
    46801 agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact
    46861 gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt
    46921 ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga
    46981 acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca
    47041 agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta
    47101 tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta
    47161 acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg
    47221 ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta
    47281 aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc
    47341 tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga
    47401 attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat
    47461 cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg
    47521 cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct
    47581 gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg
    47641 aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac
    47701 cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc
    47761 cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg
    47821 aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg
    47881 cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa
    47941 gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa
    48001 cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc
    48061 aactgaataa atcacccatc cccctgcata tctggtcgct gcaagcgcgc tgccttgcta
    48121 ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg
    48181 cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg
    48241 gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcataaa
    48301 aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc
    48361 actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga
    48421 atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg
    48481 cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca
    48541 atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg
    48601 tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg
    48661 acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga
    48721 atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg
    48781 gcgataatat ccagctgctg tttttgttcg agcgcagaaa gctcaagatc cagcaagcgt
    48841 ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg
    48901 ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat
    48961 ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca
    49021 acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt
    49081 ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca
    49141 ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagggttata
    49201 ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctggcgggcg
    49261 atattgttat tctgccaggc aatggaatgc actgagagaa tgttgtcttc ctgccgcgtt
    49321 atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga
    49381 tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt ctcctgttca
    49441 gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc
    49501 ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc
    49561 atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggttcc
    49621 cagcgttagt gcgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt
    49681 ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc
    49741 tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc
    49801 cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
    49861 ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga tcatcacctt
    49921 ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
    49981 acggcactgc ttcccacagc gttttcaacg ccaactcacc tccctgcgcg gatggatgcc
    50041 aggaaaccag ttgcgcgccg gataactgaa aatccagagg cgaaaagggc agcgtttttt
    50101 cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc tggtgatgac
    50161 ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgat agaaaaatga
    50221 gcgtaactaa catcagcaac cagaatgccc agcaaagctg gcggaggcgg ggtgatgtgg
    50281 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
    50341 tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagaagca
    50401 tcctggcgaa gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt aagccccttg
    50461 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgttc aggtaaaaga
    50521 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt
    50581 tgcaaacgct gctcacgaat ttgttggcgc tccagcaaac gtggtttcgt tatctgtaag
    50641 ctgtgggcga gttgttgttc cccctgaagc aaaacggcgt ctacgcgagc ttcggcgctg
    50701 cttgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
    50761 agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
    50821 ggcgggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
    50881 ctgacggcgg tagcggcggc tgacgaacag aaacggcctc ccagggatca aatccccctt
    50941 cgccacatat cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
    51001 tagtcatccc caccgccagt ttgcgccccc agcgatagcg tgtcgcccac agccactgtt
    51061 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta
    51121 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagagtaa
    51181 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt
    51241 cttcgctata atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc
    51301 ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc
    51361 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    51421 gactccaagg taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga
    51481 taatgtcgtt ctccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    51541 gtacgatcgc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    51601 taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    51661 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    51721 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    51781 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg
    51841 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    51901 attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag
    51961 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    52021 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    52081 gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga acgcacgctg
    52141 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac
    52201 gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt
    52261 gctgcggcac aagtctattt cggaaaaacg gtcgaccaac tgacgctgaa cgaaatggcg
    52321 gtgatagccg ggctgccgaa agcgccttcc accttcaacc cgctctactc aatggatcgt
    52381 gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccaa
    52441 caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt
    52501 gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    52561 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    52621 gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    52681 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc
    52741 gatacgctga aggcgctgcc aacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    52801 aatcctcagc aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    52861 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc aaggaccgac gccgcgtaaa
    52921 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    52981 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt
    53041 gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    53101 caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    53161 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    53221 gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta
    53281 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc
    53341 gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    53401 accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac
    53461 gcggtcatgg cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac
    53521 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt
    53581 ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    53641 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    53701 aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    53761 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg
    53821 cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    53881 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    53941 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    54001 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    54061 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg
    54121 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    54181 cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    54241 acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc
    54301 gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat
    54361 ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa acccataagc ctgataagcg
    54421 ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc
    54481 acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc
    54541 atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc
    54601 gattccgggt agagatcttg cgcgaccacg atattcattt tgctggaaaa gtaagacggt
    54661 gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt
    54721 agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc tttcgaaaaa
    54781 cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc
    54841 acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc
    54901 cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg ggaacgggct
    54961 acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg
    55021 ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg
    55081 ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag
    55141 gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc
    55201 ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc
    55261 gcagaatcat gtattgtgtc tggtgcgact gaccacgcct gacagactaa gtaagatggg
    55321 gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg
    55381 ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg
    55441 gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag
    55501 agcctgacgc aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct
    55561 ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac
    55621 ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac
    55681 ctgccccctt tctgggaaca atacatcaac gatgagaata ccgttgaact gatttatacc
    55741 gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa
    55801 acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag
    55861 agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt
    55921 cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc
    55981 tgcctgatta ccccagatgt atttgttccg tggctggcag gcggcgcatt actgctgctg
    56041 ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg agaaattcat
    56101 tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc
    56161 aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt
    56221 gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt
    56281 caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg
    56341 cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc
    56401 ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa
    56461 gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt
    56521 agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca
    56581 ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc attaccatta
    56641 ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg
    56701 caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa
    56761 tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc ggatttatgt
    56821 tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa
    56881 gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta
    56941 ttacgcccgg tgagtgcaga atcgctggat aacctggtgg caacctccac cgcgccgttt
    57001 atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc
    57061 ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt
    57121 tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg
    57181 catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg
    57241 cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc
    57301 acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca ggcctggcgt
    57361 cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga aagctgcctg
    57421 aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt
    57481 tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat
    57541 tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc
    57601 cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcca aaaacggggt
    57661 tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct
    57721 aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac
    57781 caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact
    57841 gaaagccagc ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt
    57901 aaaacttgag cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt
    57961 tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa
    58021 agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt
    58081 tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg agaaacagta
    58141 tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag
    58201 acgccatgaa agagaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc
    58261 gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca
    58321 acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc
    58381 agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca
    58441 gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg
    58501 cgatggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa
    58561 aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa
    58621 gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aattttgccg
    58681 ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag aaccacgatt
    58741 atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg
    58801 ctacgctgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc
    58861 tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg
    58921 aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca
    58981 ttaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt gataccctgg
    59041 cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc
    59101 gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg
    59161 cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa
    59221 ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag
    59281 aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt
    59341 gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga tagcatcctg gcggaagatg
    59401 gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata
    59461 ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc
    59521 cggcagagag ggctctgccg gattttacga cgtaatggcc tgatgtgctg cgcttaccag
    59581 gcctgtgaaa taaatcgcaa attattgttg gtcagataca gcgtcgtatc cgttaaaaga
    59641 gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga tcgcgccacg
    59701 ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    59761 tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc
    59821 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    59881 tattcagatc ttgctcgacc aggacgcagg cgtctttttc aatacaggtc atcacgttat
    59941 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa
    60001 ttaacacatt ttcggctaat actttttccg atacctgttc ctgaagatgt tgctctccat
    60061 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt
    60121 tcagcgactc aagcgcagca cgtccatagc gaccgctctg ctcgagcatg tagatagtta
    60181 cctggtctga cgtgtttttg ccgctctgat aacgcgccat atggctgttc acgctaatgc
    60241 gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa
    60301 ccgcaaggtt cactttgccc tgaagaacaa cgggattttg ccctttacgc cagacgatgc
    60361 tgacaaaata ccccagcgac cacaccagta acaccagcac cgccagcacg ccctgcaagc
    60421 ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg gcgacgaaca
    60481 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc cctgtccagg
    60541 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    60601 gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg
    60661 gcagagtgat gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    60721 ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgttcgc gcgaggatca
    60781 ccgccagcgc ggtaaccaga cctaacgtga gataaataaa ccagtcggtt gcaaaaaaga
    60841 gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc actccacagt aaaagcacca
    60901 gccacgtcaa gccattggcg agaaaaatca ccagtaatat aagtacgtta tgccatactg
    60961 actggtaaaa atagcgatag cggctactgt cattgcggat gcgaaggctt tgttgtatcc
    61021 acggtagcaa caacattgcc atcagcagca gatagcaacc aaaatcccaa agcgcctttt
    61081 cagctctcca ggggttcatg ccatcagttt gccacttcag ccagccgctc atccccagcg
    61141 tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa gagatcacgg
    61201 agaagagtaa aacggatgaa aacgcaacgg tcgcaggcac gccataaaca atccaactgt
    61261 gattttttcc ggcaagccag gcgatcaaca ggtagcaaac cagcccctga agtaatccgg
    61321 tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg ttggttatcc
    61381 agaatcaaaa gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa
    61441 taatcaaaaa aagttagcgt ggtgaatcga tactttaccg attgaatttg catcaatttc
    61501 attaaggaat gcgattccac tcacaatatt cccgccatat aaaccaagat ttaacctttt
    61561 gagaacattt tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag
    61621 gaatcacgga gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg
    61681 tcgcggttaa cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc
    61741 agtgaaatgc gcgttaacaa tggtttgacc ccgcaagaac tcgaggctta tggtatcagt
    61801 gacgtacatg atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat
    61861 ccgagcctga caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat
    61921 accgggatct tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact
    61981 cgcgatactt tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct
    62041 ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg
    62101 ttcgttgtcg acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc
    62161 accgaagtgg cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa
    62221 gagctggcag gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cagtaacccg
    62281 cagtggaaag aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc
    62341 atgcagctga ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg
    62401 atgaactacc tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggt
    62461 gagaaaggcg atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc
    62521 accgacccga aacgtcgcct gattggtgat gacgagcacg gctgggacga tgacggcgtg
    62581 tttaacttcg aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct
    62641 gaaatctaca acgctatccg tcgcgatgcg ctgctggaaa acgtcaccgt gcgtgaagat
    62701 ggcactatcg actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccgatc
    62761 tatcacatcg ataacattgt taagccggtt tccaaagcgg gccacgcgac taaggttatc
    62821 ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa
    62881 acccagtatc acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc
    62941 accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg
    63001 actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg
    63061 gttaacactg gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt
    63121 atcgacgcca tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt
    63181 aacctggcga tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac
    63241 acctacgctt ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc
    63301 gacaacttcg ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg
    63361 aaactgtaat gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg
    63421 gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac
    63481 cggcactggc agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag
    63541 catcccgtta tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct
    63601 aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg
    63661 cgcaattccc ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct
    63721 gctgactttg atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc
    63781 gcgtttgatc gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc
    63841 aatttcccgc tcatagccac tttcggcagc aatcacctca ccgagtactg cattaagatc
    63901 cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat
    63961 ggcgttgcac tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct
    64021 catcatctca gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc
    64081 cgccatcagc agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa
    64141 ggcacgggta acggaacgca cctccgaagc gccatactca cgcagcggcg gcggaataat
    64201 ccctttacca acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg
    64261 aataaacagc cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag
    64321 cggagagaaa tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga
    64381 cagccaggtt ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt
    64441 cgggccgccc agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg
    64501 cagacctgcc tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat
    64561 ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc
    64621 ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa
    64681 aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac
    64741 gatgagcaat aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct
    64801 ttagagccgt ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga
    64861 tgcgctggat cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt
    64921 tccattgcgg aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc
    64981 tcacgcggat ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc
    65041 ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca
    65101 attaccgcct cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc
    65161 gcacgaatac gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg
    65221 ccaatctcca ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc
    65281 ggcatcgggt tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt
    65341 aacattaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt
    65401 tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc
    65461 gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt
    65521 actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca
    65581 ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct
    65641 taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc
    65701 caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac
    65761 ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc
    65821 ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga
    65881 ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa
    65941 atgcatggaa aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt
    66001 tggcgcgtgg gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg
    66061 ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc
    66121 attgctgaaa aaagaagtcg gcgatctggc tgtggtgaat actcctgccg gggaagcgag
    66181 ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg
    66241 atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag
    66301 atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc
    66361 gcgcccggaa caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt
    66421 tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct
    66481 ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc
    66541 catttccgag caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag
    66601 caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca
    66661 aatcgccatt gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca
    66721 cacgccagaa gtcgcggctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa
    66781 agccgcgctg gacggcgcgc gctatatcct gatggaacgt tttgccgaag atgccgcgct
    66841 gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ctggtctcaa ccgtggtgag
    66901 cggtaaagaa gaggaagggg caaaattccg cgactatttc gatcatcacg aaccgttgtc
    66961 cacggtgcct tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca
    67021 gctttcgctg aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca
    67081 aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa
    67141 aggcgtagtg agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat
    67201 gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca
    67261 cgatctgctg atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct
    67321 gcgtactggg gtaaaagtgg cggtggtcga tgccactggc aaactggtag cgaccgatac
    67381 catttacccg cacaccggac aagccgcaaa agcagcgatg actgttgctg cgctgtgtga
    67441 aaagcataac gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaaccgaacg
    67501 tttctatctc gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag
    67561 cgaagctggc gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct
    67621 cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga
    67681 gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca
    67741 gacgcaactg gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt
    67801 cgatctcaac accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat
    67861 ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttcaagaacc gtcagcaact
    67921 gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat
    67981 taaccacggt gataacccgc tggacgcctc taccgttcac ccggaagcct atccggtggt
    68041 ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga
    68101 actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac
    68161 tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc
    68221 tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg
    68281 cgctgtcacc aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg
    68341 cctggttcac atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa
    68401 agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc
    68461 cctgaccatg cgtctggacg agcagcctgg cgaaaccaac gcccgtcgcg gcggcggtaa
    68521 tgaacgcccg caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc
    68581 cggtaatagc gcgatgatgg atgcgctggc ggcggcaatg ggcaaaaaac gttaaacgcc
    68641 ctgattgacc cctaaacctg cgcgataagg tgtccacgcc gcatctggca acgaacgcct
    68701 tgtctgacat aaaagtgccg gagaatatct ccggcctttt tattccacaa ccaaactcat
    68761 aatatattcc ggcaatattt atcacttcat taacaactga aaccttaatt aaacattagc
    68821 cagtccgggt aattcactat tcgaattata ttttcgctgc gatataacct tgagccacat
    68881 caacattgag tcagattatt attcaaacca acattcgcac acattttaag tattgctgat
    68941 agaaacaatt ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat
    69001 catctggtct catgtcgctg tcaaacgccc catgaggtag ttatccagtt aatgagaaac
    69061 aagtaggcac ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt
    69121 gaaatcagcc cggcatatcg ccaaaaactg ctttctcttg gcatgttacc tggctcctct
    69181 tttaatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg
    69241 agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa
    69301 tacggtgata acaacaatga aaaaattaac cataggctta attggtaatc caaattctgg
    69361 caagacaacc ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg
    69421 cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt
    69481 ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca
    69541 aatcgcctgt cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc
    69601 gtctaacctt gagcgtaacc tgtacctgac gctacaactg ctggaactcg gcattccctg
    69661 cattgtggca ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga
    69721 tgctctgtcg gcgcgtcttg gctgcccagt gatcccgctg gtttcaaccc gtggtcgcgg
    69781 tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt
    69841 gcattacgca cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga
    69901 catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag
    69961 ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa
    70021 tgagatggac gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat
    70081 ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga
    70141 taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat
    70201 gttcctgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg tcggctccgt
    70261 ggcgctattt gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct
    70321 gactatcttc ctcgcccagg gcctgggtgg cggcattaac accgtgctgc cactggtgcc
    70381 gcagattggc atgatgtacc tgttcctctc cttccttgag gactccgggt atatggcgcg
    70441 tgcggcgttt gtgatggacc gtctgatgca ggcgctgggc ttgccgggga aatcctttgt
    70501 gccgttgatc gtcggtttcg gttgtaacgt accgtcggta atgggtgcac gtacgcttga
    70561 tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg
    70621 tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg cgctggcggt
    70681 cttctcgctg tatatgctgg gtattgtgat ggcggtgctg actggcctga tgctcaagta
    70741 caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ttgccggtct atcatgtacc
    70801 acacgttaaa agcctgatta tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc
    70861 tggtaaagtg atcatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgctgag
    70921 cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgatcac
    70981 cccggtcttc aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt
    71041 tacgggcgca atggcgaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga
    71101 aaatattcag gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag
    71161 tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa
    71221 ccccattgaa gctagcaaag gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga
    71281 tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt
    71341 accgtgtatc tcggtgatgg gggctatcgc ccgtgaatca agccgtggct ggatgggctt
    71401 ctccatcctg tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc
    71461 cagctacagt cagcatccaa cttacagcct ggtgtgcatt ctggcggtta tcctgtttaa
    71521 catcgtggtt atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc
    71581 aacccgcaag ccggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg
    71641 gcttcactta ttcaggtgcg cgatttgctg gcgttacggg gccgtatgga agcggcccag
    71701 ataagccaga cattgaacac tccacagcca atgattaacg ccatgctgca acaactggaa
    71761 agtatgggca aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt
    71821 aaaagctgcc cggaaggaaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccttac
    71881 tccatcgcct gttttggaaa gcccggtatg cgtctgcatc cgggcttttt tgcgtgcggc
    71941 tttccataaa aatgcaactc ttgcagcacg gcgtaagttc ctttgaaagc atctcgcagg
    72001 gatgaaaact cgctaataca caggagtgaa gtggcgcgta gagtcgcggc attcaaacaa
    72061 caggtgaagg aacgccatga gcaaaaagca gagttccacc ccacacgatg cgctgttcaa
    72121 actcttttta cgccaaccgg acacggctcg tgattttctt gcgtttcatt taccggcacc
    72181 cattcacgcg ctttgtgata tgaaaaccct caagctggag tcgagcagct ttattgatga
    72241 cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg
    72301 atacatctat tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat
    72361 gatgcgttac gctattgccg caatgcaaaa tcaccttgat gccggataca aaacgttgcc
    72421 gatggtggtg ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg
    72481 ttggctggat tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc
    72541 gctgattgat gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct
    72601 gctggagtta attcaaaaac atattcgtca acgcgacctg atggggcagg tagagcaaat
    72661 ggcctgctta ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta
    72721 catactgcaa accggcgacg cggtacgttt taacgatttt atcgacggcg ttgccgaacg
    72781 ttcaccgaaa cacaaggaga gtttaatgac aattgcggaa agattgcggc aggaggggga
    72841 acaatccaaa gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttacaga
    72901 catcatgcgc tttaccgggg tgtcagaaga agagttggct gcggcgagcc agtaaatttc
    72961 tgtctcgcca tttcaaaagt tgcctacacc ctctgcttca acgccaccag caggtgacaa
    73021 aactcgaccg gatgcgaaat aaacggcgca tgggccgctt tggcgaagat atatgattcg
    73081 ctgtgaggcc aaagtttatc cagcatcggc accactttgc gcggcaccag accgtcgaga
    73141 tagccataca atcgcaaaaa cggcatgggc acgttttgca gcggctgacg gagatcgacc
    73201 gttttcagga tttccagccc gccattaagc acgtcaacct ccggcatcgg taacgccaga
    73261 acggttttct tcaacgcccg cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac
    73321 gccaggaacc gctccactgt acgctgaaaa tcatcactga gttgctgctg aaatcccgcc
    73381 agcacgtccg gttttatccc cggccactcg tcacgagcac taaaacaagg tgacgacgcc
    73441 acggtgacca gcgcctgaac acgctcggga tgggttaacg caatctggct tgccaccagc
    73501 ccgcccagac tccagcctaa ccaaatggct ttatcaggtg cctgttgcag cacggcttcg
    73561 gccatatcag caagtgacag cgcaccaaat ccccggctac gcccgaagcc gggcaggtca
    73621 acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca
    73681 ttcagtcccc atccgtgcag cagcacaaga tgaacattcc cctgaccttt ggtctgccac
    73741 cagatgttat tcatccgcta ttgttctctt ttgacttaca aggatgaaca tatgctaaca
    73801 gtaccgggat tatgctggct atgccgaatg ccactggcgt taggtcattg ggggatttgt
    73861 tcggtctgct cacgcgctgc ccgtacagat aaaacgttat gcccacaatg tggattaccc
    73921 gccacacact cacatcttcc ctgcggtcgc tgcctgcaaa aaccaccgcc ctggcaaaga
    73981 ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc ttatccacca gcttaaattt
    74041 tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt tactattgga agtcttacac
    74101 gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca tcagcgttcc gttatggcag
    74161 cggcgtcact ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc gttatcacgc
    74221 tggttgcact gccgatggga tagcaaagcc gtcacacgaa cacgggccac tgcgacccag
    74281 cattttctca gtgcccggct acgcaagcgc aacctgaaaa atgcctttcg tcttgaattg
    74341 cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc
    74401 gcagagattg cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt
    74461 tgtcgaacct tgtagagcct cgatgatggg cgtattataa ccaactaaaa tagtcaacta
    74521 ttaggccatt actatgatcc gtatttccga tgctgcacaa gcgcactttg ccaaactgct
    74581 ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg attaaccctg gcacgcctaa
    74641 cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg gaagccaccg acactgccct
    74701 gaaatttgac ctgctgaccg cgtatgttga tgagttaagc gcaccatacc tggaagatgc
    74761 agagatcgac tttgttaccg accagttggg ttctcagtta acgctgaaag ccccgaacgc
    74821 caaaatgcgt aaagtggcag acgatgcacc gctgatggag cgcgtggagt atatgctgca
    74881 gtcgcagatc aacccacagc ttgctggtca cggtggtcgc gtttcgctga tggaaatcac
    74941 cgaagacggt tacgccattc tgcaatttgg cggcggctgt aacggttgtt ccatggtcga
    75001 tgtgacgctg aaagaaggga tcgagaagca gctgctgaac gaattcccgg agctgaaagg
    75061 tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc tactactaag ttatcccctc
    75121 ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc
    75181 cagacgttgg cacagcattg ctttggcaaa tccctccctt cgtttccccg cgataatatg
    75241 accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc cagatgttac
    75301 ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat aattagtttt
    75361 agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa ttagagtttg
    75421 tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca tgccattagt
    75481 cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca aaatgaacgg
    75541 cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa tgccgctgga
    75601 taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc ttgccctgat
    75661 catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg cacaacgtat
    75721 cgccaccacg ctgattgcca aatttggtaa aaagcacatc cagtgggcgg tggtgttaac
    75781 cggttttacc gttggttttg ccctgttcta tgaagtgggc ttcgtgctga tgctgcctct
    75841 ggtgtttacc atcgcggctt ccgcgaatat tccactgctt tatgttggtg taccaatggc
    75901 ggctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggcccga ctgcgattgc
    75961 caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc tggcaatccc
    76021 gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat
    76081 cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga gctttggcgt
    76141 cagcgtctgg acctctctgg tgccggtagt actgatggcg atgcgtgcga ttgccgaaat
    76201 gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg acccggtaat
    76261 ggctacgctg attgccgtgc tgattgccat gttcaccttt ggtctgaacc gtggtcgttc
    76321 aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga tgatgctgtt
    76381 gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg tggacaaata
    76441 cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct ggtcgattgc
    76501 tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg cgggtggtat
    76561 cgcggcaccg ctgattgcaa cgacgggtgt tagcccggag ctgatggtta ttgcggtggg
    76621 ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta
    76681 ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa ccattatctc
    76741 ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac actgactgcc
    76801 ggatgcatcg catccggcat caaccgcact ctacttcttc ttcgctgcag ctctgcgccg
    76861 tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg actcaagcgt
    76921 tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa tattcaccgg
    76981 ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt tactgtcggc
    77041 aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc gacatcaacg atgccttatg
    77101 cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc cttgcttcgc
    77161 cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca gccccagggt
    77221 tttgcccagc gttagatccc cgctctccca gtaaccgcgt agcgtaggca ggtcatgtgt
    77281 cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg tcttctcgtg
    77341 gtcgttttcg aaatagagca ctttgtagga gtacacaccg ctgctgcgca gcttaccgac
    77401 aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat gacgtttact
    77461 ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg cgccctgatc
    77521 tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat ggtcaattcg
    77581 taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt cataggcacg
    77641 cgcggtgatg atatgcgggt ccattggcgg taatccccag ttctgcccca acgggccgag
    77701 gatatccggc ggcgcgccaa ccgacgcttt caggcaatat agttcacggt cacaccaggt
    77761 ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa tcggcatttc
    77821 atagccctgg cttatctccc agcaggcggc aaactggctg taagccagcc actgcaacca
    77881 gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac tgacgcactt ctggtgaatc
    77941 cacgttctga tacatctctg gccatgcagg ccagccccag cgcatttcgt cctctttcac
    78001 ttgctgggca tgtagcgcat caaaggctgc ctgccagaac aggctgtcgc cctgctctgc
    78061 aacaaactgg cgaaacgcgg tcatctgctc atgatcacgt tgcgcgaaac ctttccacgc
    78121 cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc agtcggcatc
    78181 gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct gagcctcttc
    78241 gctaagatgg aaatcttcaa cggcgttaac gtcgatataa atcacattca gccaacggcg
    78301 agaagacggg ctgtatgggc tggcactctc cggatttgcc ggatagagcg catgaatcgg
    78361 gttcaggccg atgaacgacc cgccacgttt tgccacatcc accagcatcg ctttgagatc
    78421 gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct gaacgcaggc
    78481 accccacagc ttttgtttat tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac
    78541 aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt gtcagcgtgt gataaccttc
    78601 cggcagcttc gtcggtagat tgaacgcttt gccccccgtt acatggcctt tgtactgtgt
    78661 tccttcttcg gtggtcagca gccagctata ttcgccgctg ccctccacca ccatcggcat
    78721 ttttttgccg ctggtataaa ccatgacatt cgggactggc gttaccgcca ctttcgtggc
    78781 ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg
    78841 tttaccgtag gcattgatgt aattggggct aatccccgcc gccagcgcgg cattatccag
    78901 acgtttgctt tccatcgagg ttccttagcg ttttgcctgc cagatacgag cctgataatc
    78961 gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga tcgccgcgcg
    79021 agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct caacataggc
    79081 tgcgaaatcc gccatcacca gatacggatc gccgccttgt ttgccgatac tgtgcagcat
    79141 ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct ctttcaatac
    79201 tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt tggccagaat
    79261 agccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac cgactttctc
    79321 ggcgatttca acgttcgcac catccagcgt accgacagta agcgcaccat tgagcgccag
    79381 tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt cggagatatc
    79441 cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga acaccacctt
    79501 caacttatcg ccaaccagtg gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa
    79561 gataatattc ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg
    79621 cggtacgcga tcagcctgcg ggttttcacg aatttctttg tacaacgcca gaatatgcag
    79681 cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat cgaaaatcgc
    79741 ctgtggattg atctcaatac cagtacgaac tttcacaaac tccgccagac ggactttatt
    79801 cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga atttttccag
    79861 attgatcagc tgatcgagat cgttagccca ctctttttgc agtgatttat ccaacagagc
    79921 cgccagtgca gggttgcact gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt
    79981 atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca ccaccagatc
    80041 cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca ggttcgccat
    80101 atgcacttgt ttatcgtgca ccaccgccag tttggcccac actttttcat cgcccggcca
    80161 ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct gcatatggcg
    80221 cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg gcatcagggt
    80281 atggttggtg taagcgaaag ttttgctggt aatggcccag gcgtcatccc agctcatctg
    80341 gtgctcatcg atcagcacgc gcagcagttc tggaatcgcg atagttgggt gggtatcgtt
    80401 cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca gatgatggcg
    80461 acgcaaaata tccgctaccg aacaggcaca ctggaagtac tgctgcatca agcgcagctt
    80521 tttaccggca gtatggttgt cgtttggata gagaacttcg gtcagttttt ccgcattgat
    80581 gccctgctgt tcggcacgca ggaaatcacc gtcgttaaat ttagtcagat caaacggatg
    80641 cgcgtgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat agccgacaac
    80701 ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc cgtctttcgt
    80761 cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga accacgggta
    80821 gttactgcgg tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa aagactggcg
    80881 gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga cagttgccat
    80941 tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg ccgggtcgat
    81001 ctcttcttcc agcaggtccg tcagattgat gtcataagcc ttcaacgaat cctgtacatc
    81061 ctgataccag ccgagattca acaggttgtt gcccgtcagg cgaccaatca aaaactccat
    81121 tgagatgtag ttaacatgtc gctgattcgc caccggcttg gcgaatggct gagcacgcag
    81181 catttcggcc agtgcttcac tcactgctag ccaccactgg cgaggagtca tttcagccgc
    81241 agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt
    81301 aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca acgttatcgc
    81361 tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc agggaggagt
    81421 tgcggggatg agcaaggaaa tgtgatctca accacttaaa gctagtgcaa accacaggat
    81481 tagcagcaaa tcaatgcaat acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg
    81541 ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta agtaaaggag tgaaacagtt
    81601 tcataagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt aagatcatct
    81661 ttttagatgg cactttcatc aggaatgaag aagaaaccct tgcttaaatg aatctgatga
    81721 acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt tgttaataaa
    81781 gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat cgcttgcatt
    81841 agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa aataatattt
    81901 cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta agtcgtccgg
    81961 ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc ggcgcgaaca
    82021 acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc ctcatttccc
    82081 agtgggcgac aggcaaaaac gatatcggct ggtactcgct ggatgaaggt gataaccagc
    82141 aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac ggtcactgtg
    82201 cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca ctcttcgccc
    82261 agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc gatgactatc
    82321 atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc catcaaccag
    82381 aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt gccaatctgc
    82441 gtgttcgtga tcaactgctg gaaattggca gtcagcaact ggcatttacc catcaggaag
    82501 cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agctgcagaa agcagtcgga
    82561 tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc tccgcccggc
    82621 agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat gccagccatc
    82681 tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg cgccattttc
    82741 tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt gtgaccggcg
    82801 aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt ttacagcgga
    82861 tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc ctgcgccagc
    82921 gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc gcagaaagct
    82981 ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca ggcgatgcgc
    83041 tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat agcgaactgt
    83101 cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa aatccgcagt
    83161 tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa gttaacaccc
    83221 tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg cacgcagaat
    83281 ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa gcggaacggc
    83341 tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc attgtggcaa
    83401 cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca ctggcgctaa
    83461 tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc ttatggagtt
    83521 taatccagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg tgggaaacac
    83581 aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgccg atgcatgagt
    83641 ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat gaagccgaag
    83701 cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa cagcttcagt
    83761 gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac gcccgtagcc
    83821 agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac tggatctcta
    83881 acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc gccgctgcca
    83941 actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg caaggtcaat
    84001 ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg gaaattgttc
    84061 tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc aaccgtaacc
    84121 tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc cagcgcgtgt
    84181 tgctggacgc attaaaactg gcgaatcgca ccggatttat cagccatttt gtcatcgaag
    84241 gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg ccggaactgg
    84301 aacagcatcg cgcacagcgt attctgcgag aaatcaatca acatcatcgg cataaattcg
    84361 cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg ccagaactga
    84421 tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt tactccggtt
    84481 acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc aagacgcata
    84541 tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg cagcacgcgc
    84601 agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat gcattggatc
    84661 cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt ttctatcaaa
    84721 ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag atggcatgaa
    84781 ggatgggcga ccttaaattc tcccgcgccc gccagcgcca tcggcagtac cagttggtcg
    84841 gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc tttcactaac
    84901 tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa gcgttcagtg
    84961 atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc gcgcggcagg
    85021 ttatgaatat tctgttcatg cagggaaaaa ctccccgcca gtgtagcgat ttcacgctca
    85081 gcaacatggc gcggcacacc agctaataga acctctccac gcatctgcac aatgttcccg
    85141 cgctcgccaa gttgcaaggt gttaaacgat gccacgggcg agacttccgt tgccaccacg
    85201 ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg aattcctatt
    85261 ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggggc cgacgggtta
    85321 tcggtgcctc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag cgcgggcagc
    85381 accgtttgca gcaccagcgt acagcttccg gcactaccga tagcaaagcg gtactcgccg
    85441 ccgcgcacga tgccaggccg gaagaccaga cgctgcgacc ccagctccgc gccttccacc
    85501 gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg caacagcccc
    85561 ggtttcgccc gcccggcacg aatgctggtg atggtaaatg gctggccggt tatcatcgac
    85621 aggctcagcg ccgagcgcag gctctgcccg ccaccttcgc cctgtgcgcc atccagcgca
    85681 atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt atagataact
    85741 tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc catcgggatt
    85801 tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg aatttgatct
    85861 tccacgctga acagtttttt cgctttagta cggctcatta cccgcccggc accgtggctg
    85921 cacgaacaga aagattcttc atttcccagc ccacggacga taaagctttt tgctcccatc
    85981 gaccctggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt acgcgtcacg
    86041 tagatctctt caccaaagtg ctgttctttt tgcacatagt tgtggtgaca gttgatctct
    86101 tccatcgcca gcgtttgtgg ctgtctgacc gttttctgcg taatgctctg caatgccgtt
    86161 accacgtttt ccatcatcgc atcgcggtta aggctggcaa aaagctgcgc ccaggccacg
    86221 gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag atcacgcgac
    86281 ggcaacgtct caagcgtttc ctgcatctct ttttgtgcca gatcgataaa gtaagtcccg
    86341 atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg gtccgactca
    86401 tcaaggcaga tttcaataaa gtggttaccg gttcccagcg ttcccaggtg tttatagtta
    86461 ttggtattca ggaaacgggg atatttttgc gttaaccact gataaccggc ttcaagctca
    86521 gcccatttcg catcgacgtt aacaggcggg ttttcccatg cgcctttatc acgtttacaa
    86581 cggccagtgg tacgcccgtg cggcacggcc atttcaatcg cctgacgcat ctctgccagg
    86641 ttttcaggca ggtcttccgc cgttaacgcg gtacgcagcg cgttcattcc acagccaata
    86701 tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct accaatggtg
    86761 gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat aaacggcatc
    86821 ttcgccgtat taataagttg ctgacgcgca tcggcttcta ccggcacgcc tttggtccac
    86881 atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt tttctctttt
    86941 cgttgtttgc tgtcgtgata attgcaaccg tcgtgccaga aaattaaata agcagattaa
    87001 ttttttaatt cattgttttt aaataggtta aatctttacg tccgtaaacg gagattttcc
    87061 acaaaaccaa ttcaccgata atgaaatatc gtcttttata aggatatcta agatgcgtaa
    87121 aacagtggct tttggctttg tcggtaccgt gctggattat gccggacgcg gcagtcagcg
    87181 ctggtcgaaa tggcgtccga cgctttgttt atgccagcaa gaatcgttag tcatcgatcg
    87241 gctggaattg ttgcacgaca cccgctcgcg ctcgctattc gaaacgctta aacgcgatat
    87301 cgccagcgtt tcgccagaaa cagaagtcgt gggcgttgag attgaactgc ataacccgtg
    87361 ggatttcgaa gaggtttacg cctgcctgca tgatttcgcc cgtggttacg agtttcagcc
    87421 agaaaaagaa gactatttaa ttcacatcac caccggcacc cacgtcgcgc agatttgctg
    87481 gtttctgctg gcggaagcgc gttacctgcc cgcccggctg atacaatctt cacctccgcg
    87541 caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg atttaagccg
    87601 ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg attttcttaa
    87661 gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga tcgaaaaagt
    87721 ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg gcaagtcatt
    87781 tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg gcgcgtttgt
    87841 ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt ttggtcatgt
    87901 aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca gcgccaacgg
    87961 cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacagg caatgctgct
    88021 aaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg tgagcagtga
    88081 ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg aaggcaaatt
    88141 tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg gtctacgcca
    88201 gcgccaggaa gatattgaac cgaaccttga ttatgaagtg gagcgccacg ccacactcac
    88261 tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt ttgcgacctc
    88321 tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca cgcggatggc
    88381 cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga taaaccgtct
    88441 gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg gcgcagaggc
    88501 agaaaacatc gacctttttg acaggctgca actggaacac gtgatagctc tctgtcgcca
    88561 ggcaaagtcg ctttccgctg ccggacgcct gctttttgat gtttcgcgcc agggcaaagc
    88621 cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacgtggga
    88681 agccgtgcag gatcagcaca gctccagttg aatatgatgg tccgtcagca cctgcattac
    88741 gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc tgcccatatt
    88801 gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt ggcgcgagtt
    88861 ctcaataatg gcgcgcttgg tgcgaacttc gtggtaatcg aactccagca gcgagccgtc
    88921 gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc
    88981 gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcgagaat
    89041 aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt tggtgacaat
    89101 acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc
    89161 gataaacagt gtcgagccgt tggggatttg ctccgccact ttgcgggcaa tcctctcttt
    89221 ttcttcggtc tgggtggctt tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag
    89281 cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat tgaggtcgcg
    89341 gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac tgacataacc
    89401 ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca tttataaatc
    89461 cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca
    89521 cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc
    89581 cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa atgccacttt
    89641 gcggatcgcg ttcaccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa
    89701 gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa
    89761 tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca ccgccgagat
    89821 accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg
    89881 tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc agccataaca
    89941 tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag gcgatcatca
    90001 ccacccaggt taccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat
    90061 aatgcaggcc actgccggta tggcctgcct gccagctggc cgccagataa cgcggatctg
    90121 ccgggttttc gagaaaacgc gccagctccg cccgtacgcg ctcggcctgg gactcatccg
    90181 ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgcgtcgcca
    90241 tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc atcaacatcg
    90301 ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac
    90361 ctctgcggga aactgacgtt gccaggcttc aaagccgccg tcaatgctat agaccacatc
    90421 gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt gataacacat
    90481 caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg tgtcgttggt
    90541 taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc gaatatcgac
    90601 cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa
    90661 ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg
    90721 tcgaagcggc agataaacgc cataatgtta tacatatcac tccaaaatgt tttttcaatg
    90781 ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacacttatg agctttaacg
    90841 aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat
    90901 ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg
    90961 caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc
    91021 taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg tgaagtgctg
    91081 ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca tcgtccgcat
    91141 ctgcgcccgg cgtggatgat tcggattggt ctgtttatgt acgatcatct gggtaaacgc
    91201 accagcctgc cgggatcaac cggtttgcgt tttggcgcaa actcagtgtt aaaaccggaa
    91261 attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc
    91321 aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg cgccacctct
    91381 gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa
    91441 tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa acagttcttc
    91501 gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt
    91561 gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga agataaacgt
    91621 attgtgttcg tgatcccgtg gatggacgaa ttttccatca tcggcaccac cgacgtagag
    91681 tataaaggcg atccgaaagc ggtgaagatt gaagagagtg aaatcaacta tctgctgaaa
    91741 gtgtataacg cgcactttaa aaaacagctt agccgtgacg atatcgtctg gacctactcc
    91801 ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac ccgtgattac
    91861 acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt cggcggtaag
    91921 ctgaccacct accgaaaact ggcagaacat gcgctggaaa aactgacgcc gtattatcag
    91981 ggtattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat tgaaggcgac
    92041 cgcgacgatt acgcagctcg tctgcgccgt cgctatccat tcctgactga atcgctggcg
    92101 cgtcattacg cacgcactta cggcagcaac agcgagctgc tgctcggcaa tgcgggggcg
    92161 ataagcgacc tcggggagga tttcggtcat gagttctacg aagcggaact gaaatacctg
    92221 gtggatcacg aatgggtccg ccgcaccgac gatgccctgt ggcgtcgcac aaaacaaggc
    92281 atgtggctga atgcggatca acaatctcgt gtgagtcagt ggctggtgga atatacgcag
    92341 cagaagttgt cgctggcgtc gtaaggatga tgcccggttg aagtgcaacc gggctatttg
    92401 gattccttag actattcatt gtaaataatt gaataacggc ccctgagcta aaatcgccaa
    92461 acttaagcac gttacccaat cgccccactt cttcctctgg tttctatttt ttctgcacca
    92521 atggcaataa tatcccaatg caaaaccaag ctgtgcacta atcgggccga caaaatcagt
    92581 ccaaggctgc ggccaaaaag gggaaaaatt caggggaggc cagacaaacg tcagaaagaa
    92641 atttcggata ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga
    92701 gagaattaac cagaaataga ccggcgaaaa gaccagccag caatgcagtc tgttttttaa
    92761 ttcaggaggc gttctgcggg agaattgcca accgatgcca gccattacac tgaaaaaaag
    92821 tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacgca caaccaggag
    92881 cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta cggctaagac
    92941 aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta cagggggaat
    93001 ataaagccgg aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc
    93061 gcgcataaac actcatcaat tatccagtcg tctacccaga cctgatgctg tgtgccctgt
    93121 tctggctgct ttggtgggtt ctcaatcgcc tgccgcgcgc gttctttcaa gatggattga
    93181 ttgttgaagg cccaggcatt gatttgggtg gtaatgacgt tatcgatatc gtcctggtta
    93241 tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac ggtcgaccaa
    93301 aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc ggcacggaaa
    93361 aacatttcca gcatatactg taattcgaca gtttcatcgc cggataccca atcagtcata
    93421 tactggcagg caaatcgttg caaaattttc cattcatcct cacgccaatt ttctttactg
    93481 gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg cccctgtgca
    93541 acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg cggatcaggc
    93601 cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtactc
    93661 cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc
    93721 ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg catggcgggg
    93781 ctgggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga tagacaaaac
    93841 gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag ctggggcgaa
    93901 tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag taatgacgcg
    93961 tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta ttgttatgaa
    94021 atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag gagtcacatt
    94081 ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc gccgtaaatt
    94141 gtacagtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca tcactgatat
    94201 aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg gcggtatcga
    94261 gggaaaatcc cggccataca ttgctggacg ttaagccaat atttaatgtg acattatatc
    94321 gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc agggcctggt
    94381 taatgagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa
    94441 tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta ttaactaatt
    94501 ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa cctacatctt
    94561 ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt gtctctgacg
    94621 tcgtattttc ccgcgttacc ataaaggcat cgacgatata gggatatttg ccgttgcgaa
    94681 tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcgtcgt
    94741 tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta ttacggtatt
    94801 cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc tgcggcgaga
    94861 tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca gagtcttccg
    94921 ggcgttgcca ggtctggatc tcctgatcgt taccgccaac cgtgcgccag ttggtaattg
    94981 caccactgaa gatgtcacgc acttgttgtt cggtcaggga attaaccggg ttatctgcat
    95041 tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta aatcaaagtg acgcccgatt
    95101 cctccgcgcg ttttttctgc ccaccggaag gttgagccac aaagataata tcggcattac
    95161 ctttaacaat cttgttatat gcttcgggag tacgagagtt cgccagatat tcccattcgt
    95221 gaaaatcttc cggcaaaacg cttagtgcat aaaaggcaga ggcataaatg gggtacgccg
    95281 ccgttgcgcc atcaagtcgc ggccagtttt gcgtgaactg aatttgcggt ttaccacgca
    95341 gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca atattttcac
    95401 gcacaccgac gccgggatta agctgtttat ataaataagc ctggtaaccg gcgacaaaac
    95461 caagcatgag aaggataaac aagagagaat tacggcaacg taacccggac gtgcccgtta
    95521 aaggatatct ggtcggccag ccgtacccca gcgaaaaact gatatgcgcg ccaacaggta
    95581 taatgactaa ccaccagtac tcaccaccga ctaatgaggc taacaaaacg cccagccagg
    95641 ggagcataag atcagccaac caacccgctc ccatctggtg aaattgataa tctgccagat
    95701 gcagcgcgat tatccagacc cccagggtta ataatgcggg tactaaaaaa agacattgtc
    95761 gccagacggc tatttctctt ttttctggtg cactatcgtc cgcccacact cgccccgaga
    95821 aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg taaattgttg
    95881 gcgtgataaa gatattacaa acttcaataa taccggcaag tccgacaccc agcatggcaa
    95941 taaccgccgc caaaaactgc gccagaatgg ggatgccgaa aaaagtcatt accagcgagg
    96001 tcaaaatcca ttttctgttt tgcattattc tttccattct ttctgaatgg cgaaattata
    96061 ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg
    96121 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc aatatgccag
    96181 atatgatcgg cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
    96241 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcatc gactttatcc
    96301 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
    96361 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
    96421 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
    96481 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaagataaa gatattgtca
    96541 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
    96601 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc
    96661 tgttcagaca gatcggccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
    96721 atgaacacga ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg
    96781 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
    96841 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
    96901 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
    96961 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
    97021 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
    97081 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
    97141 tcgtccagca cggctgaaag cgatgggttc gctaccgcta gccagcgacg cggcgtcaca
    97201 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
    97261 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
    97321 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
    97381 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
    97441 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
    97501 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
    97561 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
    97621 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
    97681 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag
    97741 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
    97801 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
    97861 aatttaccga ggttaatttc gctactggct tgggcactcc acaaacgcaa cgtgttggtc
    97921 gcgtcggtat cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
    97981 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
    98041 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
    98101 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
    98161 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
    98221 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
    98281 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
    98341 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
    98401 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
    98461 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
    98521 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
    98581 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
    98641 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
    98701 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
    98761 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
    98821 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
    98881 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
    98941 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
    99001 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
    99061 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagacccgg taaggcttcc
    99121 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
    99181 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
    99241 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
    99301 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
    99361 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
    99421 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
    99481 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
    99541 gcataaaaca tgccttgata ggccaagttg tgcacagtaa acaccgactt cgccggacgc
    99601 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
    99661 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
    99721 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
    99781 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
    99841 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc
    99901 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
    99961 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
   100021 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
   100081 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
   100141 cttcctctgc gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga
   100201 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
   100261 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
   100321 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
   100381 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
   100441 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
   100501 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
   100561 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
   100621 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
   100681 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
   100741 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
   100801 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
   100861 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
   100921 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
   100981 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
   101041 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
   101101 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
   101161 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
   101221 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
   101281 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
   101341 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
   101401 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
   101461 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
   101521 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
   101581 aggttgagca tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt
   101641 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
   101701 accactgctt gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt
   101761 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
   101821 ggagagcaac aacgttgtta acagggcgtg aatgctgtcg cgccgccgtt caaccaggtc
   101881 aagagaaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
   101941 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
   102001 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
   102061 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
   102121 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
   102181 cgggaaattt cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt
   102241 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
   102301 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
   102361 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
   102421 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
   102481 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
   102541 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
   102601 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
   102661 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
   102721 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
   102781 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
   102841 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
   102901 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg
   102961 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
   103021 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
   103081 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
   103141 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
   103201 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
   103261 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
   103321 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
   103381 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgta taccgtgccg
   103441 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
   103501 ttgcccggct ggtttatgcc gaagcgataa tcatgacgcg gtaccggcgt aaagttactg
   103561 gcaacgatga tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
   103621 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
   103681 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
   103741 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
   103801 tgggcaaatt cgttacccat gaacagtagt ttcttgcccg ggaatgccca catccagcca
   103861 tagtaggcgc gcaggttcgc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
   103921 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
   103981 ttgtagagaa tcccgaaggt cagtttatcg tgatgatact gacgataaac cgggtcgagc
   104041 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
   104101 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
   104161 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
   104221 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
   104281 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
   104341 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
   104401 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
   104461 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
   104521 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
   104581 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
   104641 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
   104701 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
   104761 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
   104821 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
   104881 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
   104941 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
   105001 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttaaattgc
   105061 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
   105121 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
   105181 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
   105241 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
   105301 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
   105361 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
   105421 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
   105481 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
   105541 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
   105601 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
   105661 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
   105721 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
   105781 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
   105841 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
   105901 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
   105961 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
   106021 cttccacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
   106081 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
   106141 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
   106201 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
   106261 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
   106321 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
   106381 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
   106441 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
   106501 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
   106561 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
   106621 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
   106681 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
   106741 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
   106801 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
   106861 ccataccgcg ccagccgata aaaccaacat ttttcataag cgtttttttc ctgcaaagat
   106921 gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac aaaaagcagc
   106981 caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg acttatcctg
   107041 ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta tgaatgaaat
   107101 catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc tacctatttt
   107161 catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt
   107221 gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt
   107281 tctcagcctg cgagcagaaa ccgtctccat ttctggcggc atcattctgt ttctaatcgc
   107341 cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg caggtgaaga
   107401 gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc tcgccacgct
   107461 gatgttgctg tctcaccagt acccaaatca gatgggacat ctggtgattg ctctgctgct
   107521 ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac gtctgctggg
   107581 cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga tgatggcaac
   107641 ccagatgttc ctcgacggca ttagaatgtg gatgaagggg taaggttcaa acaggtgctg
   107701 cacacaacca gacgcggcac ctgttttatt acctgaactg ataatgacct tcgcccaatc
   107761 ggataaccct tccctcttct tctgcgcggt tcagccacac actaatttgt tcgatggtta
   107821 aaccagtaca agacgacagc ctttcaatac ttatcgattc ctttgcaaag atcgccagtt
   107881 ccgccacaaa gagttgataa aaatcgacag gcgtatgcgc gggcattact gcgtaatttg
   107941 cgtcattttc atgcagcgaa aacagttctg gttgagcgtt acggagtgct ctgtcataca
   108001 tcagcccagg aacgtctgac agtctgatcg gcacattctc agcctgttca gcactccatg
   108061 acgccccatt ttctactaat cgtgaattgg ctgattgcat atcctgattt ggtttaacct
   108121 gcacaggcag ccactgatgt tttaatgcct ctatcgcccc ggttatcgtt ccaccggtca
   108181 ttcccgcacg tacaaccatt gcgctttcag caaggcaata aatatattta tttcgcgcca
   108241 tcgcattgcc gacggtgaaa cgcacctctg ggtaaaacgg cgaaatcaac accaggttgc
   108301 ctgctataag cccttcacgc catttcacta acgtactcgt ttttaacaag ctatcagcta
   108361 atacgccaac ggcagttccc ccggcctcca gtgccgacgc cattgcacat tcatcaatac
   108421 ctcgcgcacc accagagata acgcaaatcc cctgttgggc cagtttcgcg gccagttgtt
   108481 gggtatagcg caaatcgtca gtcggagcat ctcgcgagcc aacaatcgcc ataccttccg
   108541 cttgcagtaa tgctttattc ccgcagccaa ataaaacggg aggtgcatcc gttcgcaatc
   108601 ggtttttcaa gcgaacggga taatcagcat ctcctcgggt tatgatccat aaaccggcgc
   108661 gttgccactt atctaccgcc aacgccagac tatgaccgcg cgccagcaag ccaagaatac
   108721 gctcctgaga aatacgcgga tcgctccagc ctgtaagttt ctcttgcggt tgcggcacca
   108781 gaagctcggc gggactgata cgttggtgct tcagccataa tgcaaacttc ccccattcac
   108841 tattactaag aggtttatgc tcactcgccg ccgcgcgaga aaaatcgctg gttagcagga
   108901 gagtcgcttg tgcattggct gaaagattca tcaatttttt accgaggtag aagcaagggc
   108961 aagaggataa accgtcgggc aacctgcctg gcgtaacagt gctgtcagaa ctgtcagcgt
   109021 ccatgcagaa tccacgatat cgtcaaccag caacgccgga cctggcatca aaggaggggt
   109081 aatcacaaac gccccgtcga gattttgaca ctggtgaaaa cggttttgct gcattttctg
   109141 cggtggattg tccacgactt tttcaatggc atcaataaaa ggtaagccaa gtttcgccgc
   109201 cagtcgccgg gcaaaatcag gaaccaggtc gaggtgcctt aatgaaggaa cgcagcatac
   109261 ccaggttggt tcaggatgcg gattccagcg ttggcgaacc atttccgcac atgcctccac
   109321 cagctcatca ctgaatcgcc ccgttttttt ctcctgtgct acctgctttc cccatccgga
   109381 atctccccaa cgagaaagga ctcgccccgt agatccttgt aaacccgcag gcaagttccc
   109441 tttaaatccg tattgcgtaa aagctccaga tgctacctgt ttattgagat ttaatggcag
   109501 gtcagcgtgt ttaataaata aatttgccgc atggagtaag ccgctatcaa tatcgggcga
   109561 caacaacgga tgctgaagac aactgctaca tttaccacaa cgttcggcac ttggctcatc
   109621 gagtgcgcgg cgcagaaatt gcattttgca ctccttggtc gtgatatagc tttcaacatc
   109681 tgccagctca ttttttctct gattcatcaa atggttaatt cgttcatggt caggagagaa
   109741 actgacgata gttctgcgcc ataatttctc ggtatacacc acaggcgatg gattttcagc
   109801 taccaataat ttcagtgctt tttctatttg cccataacgg agattcgtcc gttgttcaat
   109861 gcctcgtagg gtaagaccgt cattctcgct aagtacgttg agtatttcat gaatttgcgc
   109921 ctccgcagga aaggcgcttt cacggaagaa tttatgaata gcgcggtctt caccaccaca
   109981 aagcaatatg ccaacagcgg aatctatagc acgcccggca cgccctacct gttggtagta
   110041 accgacaata gaccccggca tctggtaatg aataacaaac cccaaatccg gtttgtcaaa
   110101 gcccattccc agcgccgtcg tcgcgaccag aactttaatt ttatttgcca gcaacgcctg
   110161 ctccagatat tccctggcag tattagaatc ttccgcgcct tcacaagtca cgccgctgta
   110221 gtaagcaaat gcactgatac catttgtcct taaccactcg gcgacaaggt cggcatcgcg
   110281 aggagtcagg gtataaacaa ttcccgattt ggaaaactga gggataaccg ttgctaacca
   110341 tgccagacgg gatgactgtt cacctaagac taaggcatca agcgccaaac tttcgcgagc
   110401 cagcgttcca cgctgaatca caatgtcacc caattgctga cggatatcct caacgacacg
   110461 gttattcgct gtcgcggttg tacccagaac tggggtattc gcaggcagtt ggcgcaaaat
   110521 atctaatata cgtcggtaat ccgggcgaaa atcgtggccc caatcagaga tgcagtgcgc
   110581 ctcgtcgacc acaagcaagc caatacgatc ggctatagga tataaaaccg tttcgataaa
   110641 gtcttggttt gccaaacgtt ccggggaaac cagcaagcaa tcgacctcac cttgcagcaa
   110701 cttatcgcta atacgctgcc attcttccct gtttgtagaa ttcaacgttt cagccgtaat
   110761 acctaaccgt tcagcggcgg caacctggtt acgcatcagg gcaagcaaag gagagatgat
   110821 aattgtcggg ccagcaccgc gatcgcggaa gattttactg gcgatgaagt acacggcact
   110881 tttcccccag ccagtgcgtt ggacaaccag cagttttcgt cgttggttca ccaccgcgtc
   110941 tatggcttcc cattgccctt ccctgaaagt agcggtggta tttcctaaca tccgctgtaa
   111001 aagcaattca gctgcgtgct tttccatctt tatctcctgc gatctttagg cagtgattag
   111061 aacggattat tagcaggtaa atttgcgaga gggatcaaaa agatgaagag aggagataag
   111121 agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa ctcaacagct
   111181 ggaacgcaat catcccaacg atggcaccga cagtgccgag gatggtttcc atcatggtcc
   111241 aggttttcag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg
   111301 cgtcgttaac gtggctgaca acaatcgaac caccagcgat acaaatcgac agcgccgcca
   111361 tttgcgcacc ggagtagttc agttgttcaa taaccggcat caccagtcct accgccgtta
   111421 aacaggctac ggtggcagaa ccctgaatga tgcgcactgc agctgccagc acgaagcagg
   111481 tgatagcaat cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag
   111541 agtcaaccag cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg
   111601 ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca
   111661 ttgccaggcc ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa
   111721 taaactcgaa ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt
   111781 tcagccccac cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca
   111841 ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc
   111901 ggctgatgaa attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca
   111961 ggccaatcag gatcatccag ccaaaatcgg cgttcatctg cgatgccagc agcattggcg
   112021 ctggccctgg caccaggaac gccgccgctg ccgccacgcc tgcgaataat gggattacca
   112081 gcttcaccag gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa
   112141 caatcgccac ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat
   112201 aatgcgcgcg gctgtgaccg aaggatttaa gcattttgac ggcaatctga tcgactgcgc
   112261 cggtttcatg taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc
   112321 cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg
   112381 aaaaaaggcc agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct
   112441 tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg
   112501 tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca
   112561 gcggttgatc gatatccacc accagtacat cagtttcgtc cgcacccggc tcctgcagcg
   112621 tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc
   112681 ggctttcaat cacgtcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt
   112741 cacgcagcaa gtcgcgatag tgttttttca atgcagaaca gacgatcagt gacactttat
   112801 tagtgcgctg catagcaaac gcggcgtcgt tcagcgcctt caaccacggt ttgcgatcgt
   112861 cgtcattcag cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat
   112921 cgccatcaag aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc
   112981 cgctgcctga gacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag
   113041 tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt
   113101 ccggccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta
   113161 cttcaaattt gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca
   113221 ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc
   113281 ccatccgctc acgcggcgtc agcacgctcg ccagtcgtgg ctccatcacc tgaccaatgt
   113341 catgaccgtg gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac
   113401 attcaaacgc cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg
   113461 gatattcccg ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct
   113521 caaccatcac gctatacggc accaggcctg catccagcat cgcctgttcg tatcccttct
   113581 gtttgatgat agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc
   113641 gcgcaataat ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga
   113701 tatcgaggca cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa
   113761 tcatctttaa ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc
   113821 aggacagcat ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca
   113881 gcatcgtctg ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga
   113941 aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga
   114001 ggatatcggg cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc
   114061 cgcgtagagc gacggaaacc tgctccgggt tacgcaaaaa gcggctgacc gtcattttgg
   114121 tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga
   114181 aggtacaaaa gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga
   114241 aaacagtgct tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta
   114301 acgacgcggc aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc
   114361 tgcaaaaagg caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt
   114421 aacacttcgc tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga
   114481 ccatcgctgg tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc
   114541 cagacgcggc gttcagcggc aatctgatgc accgactgct catctttcag caacgcccaa
   114601 cgatacagtt ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc
   114661 accagctgtt tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca
   114721 ttttcttcag gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg
   114781 tactctgagt gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc
   114841 tgctctttat tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa
   114901 atttccatat ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg
   114961 ttaatcacgc gcagcgcgga aaagcccata aagttcggat cgtaatagtt ggcaaaagag
   115021 aaagtatgcc aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt
   115081 aagtagatca tttgtattcg ccccatgaat gatttcgatg ggcttagtgt ggacccgatc
   115141 tgcctgggat gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga
   115201 ggggccatca aacttactta gcgagagtta ctgtggaggg agaggcttgc tcaaatccgc
   115261 gttcaaggat ttccagattc gtaagaactt cagattcctt gacgtaattt ggcgcaccgt
   115321 gggtgatggt ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca
   115381 tctcttctct gaccgtcacg ccctcgtcat tgacatactc cagcacaccg accgaatcat
   115441 ccgctgcgaa tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt
   115501 cgataccgta tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga
   115561 ttttcaccag atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt
   115621 caaaggtgtc gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat
   115681 ccgggcgacc gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg
   115741 cgccatcctg cggcagccca ggtttggtct ctgccaccgg gcggtaatag tcaaaatggc
   115801 tttccacttc aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga
   115861 agcaggagtc aaagcgacga ttctgatacg gcgtgacggt cagccctttg cttttcgcca
   115921 acgcaaacag ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat
   115981 ttttcccggc ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga
   116041 caacaaccag cttaacatcg ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga
   116101 tatgggaata aatgggagcc tgttcttccg gcttcgcatg gtgacgaaaa atatgcgcga
   116161 catgccagct atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc
   116221 cgaagccaat aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta
   116281 caccttagcg caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc
   116341 ccgtggataa attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat
   116401 actggaagca atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg
   116461 cgagacaata ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat
   116521 ttgactcgca tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag
   116581 gaatgagtga gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga
   116641 ttcacgccca gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata
   116701 tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg
   116761 gagacgtcgt gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg
   116821 ccgattttgg tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga
   116881 tgcgagagat gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg
   116941 tgtttgtcga taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag
   117001 ggactggtaa gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc
   117061 gggtgaagta agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac
   117121 ataccttcct ttaaggtatt tatttgccca atacatatat tgagatttaa ctcataatta
   117181 cttcctaaag tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat
   117241 aaataacaat taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt
   117301 ttcagggatg agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc
   117361 cgcaggttgt gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga
   117421 aatatttaca ttctcactct taaataatat taataatacg gggcttggtt cacagttcca
   117481 tggtataaca ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa
   117541 caataatgaa caattattta tgggatttga cttctatcga ataaatagat ttggtagatg
   117601 ggaaaagtat tattatatac aactaagagg cgctttttta tcggctattc atcaccagat
   117661 cattgaaaac caactggata cagaaacaat aactattagt tatgaattta tcctctgtca
   117721 acatcttatc gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgttt
   117781 gtttttacca aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc
   117841 tattggcagg ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga
   117901 tactgcggaa aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa
   117961 aacagcgggc atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga
   118021 tacatataca gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga
   118081 tggtatagac ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt
   118141 acgaaaggag tttaataaaa ctgtaagaaa aaattttctt atcaaacttg caaaaacctc
   118201 cgaagcatct ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc
   118261 tctaaattat aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga
   118321 aaatttagta ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg
   118381 aatagctaag ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg
   118441 ctcaatttat cctcccatga aaaatattat ggaccacaca aaatgatgac taaaacccaa
   118501 ataaataaat taataaaaat gatgaatgat ttagactatc catttgaagc accgctcaag
   118561 gaatcattta ttgaaagtat aatccaaata gaatttaatt ctaattcaac taattgcctg
   118621 gagaagttat gtaatgaagt tagtattctt tttaagaatc aacctgatta tcttactttt
   118681 ttaagagcaa tggatggatt cgaagttaat ggattacgat tatttagcct ctcgattcca
   118741 gaaccttcag ttaaaaacct ttttgccgta aatgaatttt atagaaataa tgatgatttc
   118801 ataaaccctg atctacaaga acggttagtg atcggggatg atagcatttc aatatttact
   118861 tatgacatta aaagtaactt ttttgaaatt agagataata ttggcacaga aaatatattc
   118921 agttcgttta gtgatttctc ttcattctta aacgaaatca tggacagttg ttcataataa
   118981 tcaaaaggaa acaaaaatca ttgtaaacca tgccatcttt ttagagatta ccagcagaaa
   119041 attccttgtt cgaaatttag cagtgcaagc tatggaaaaa cttacaacgg aactacactc
   119101 cttaagtgag atggaccgaa gacatgtagt ctctatttta gccgaaatag ctaatggcta
   119161 tgatgatttt aatgacatgt tgatttattt agaattttac ccgaatcata agattctgat
   119221 atcttaaata agaaaatccc gttcgacgtt ctgaaaaaga aatcataaaa tattagcaga
   119281 tacttatttc gtgactatat cttatccgat gaactttttt caaaaccagc atttaataat
   119341 atctttgacg aagatgaacg cgtctttgct caatgtatca cacaggaata ataatataac
   119401 actcaacaat aatatttatt ataattatga ttacttatca cgacgcattc gcgaaagcga
   119461 accattacct tgatgatgca gatctcccgg tcgtcattac tctacatgga cgctttagcc
   119521 agggctggta tttctgtttc gaagcacgag aatttctcga aactggagat gaggccgcgc
   119581 gcttagctgg taacgcacct tttattattg ataaagacag tggtgaaatt cattctctgg
   119641 gaacggcaaa gccgctggaa gaatatctac aggattacga aataaaaaag gctaccttcg
   119701 gcttgccctg acaaaatagc cctcttccca cgaagagggc cgctaacctt agtaccccgc
   119761 cgttaaatca tccaccgagc gcgggtcgga tgcgccgtac aactcaccgt ccggcccaac
   119821 cataatgctt tgtgtactgc ccatcgcctc tttcagcgcc actttctgac cttttgcttc
   119881 cagcagcttg agcgtatccg ggctaaaccc tttttcgaca cgcagctcgt ccggcaacca
   119941 ttgatggtgg aagcgcggtg cattggtcgc ttcagcgaca ttcatgccat aatcgatgct
   120001 attcaccacc atttgcagca cggtggtaat aatccgactc ccgccagggc taccggtaac
   120061 cagccaggtt ttaccgtctt tcaccacaat ggttggtgac atcgacgaca gcgggcgttt
   120121 attcggcccg accgcgttgg catcaccgcc caccagcccg taaacgttcg gtacgcccgg
   120181 tttggcggag aaatcatcca tctggttatt aagcagaata ccgctctcgc ccgcgacaat
   120241 acccgtaccg aaggtggtgt tcagcgtata ggtcaccgcc accgcgttac catctttatc
   120301 caccactgag taatgggtag tttgattact ctcataaggc gcaagcttgc cagggcgaat
   120361 ctcgctggac ggtttcgctt tattgatatc gatttgctcg gcaatagatt tggcataggc
   120421 tttattggtc agcgcctgcc acgggacttt gacaaaatcc gggtcgccaa gatattccga
   120481 gcggtcggcg taggcatatt tctcagcctc tgccatgatt tgcatcgcat cggcgctgcc
   120541 aaagccgtat ttcttcatat cgaagttttc cagaatattg aggatttgta cgatatggat
   120601 cccgccggag gatggcggtg gcatggagta aacctgatac ccgcgataat cgccgcttat
   120661 cggagtgcgt tcgaccgctt tataggccgc taaatcttct ttagtgatca agccaccgtt
   120721 tttctgcatc tcctgggcga tctgttccgc aatcgtgcct ttatagaatt cgtccgggcc
   120781 gttttcagca atcatctcca ggctctttgc caggttcgcc tgcaccagcg tgtcgccctt
   120841 tttcagcggc tcgccctctt tccagaagat agctttactg ttttcgtgat tcggcaacac
   120901 ttcgctaccg taggttttga gatcgtcagc cagcgcgtcg ttaacgataa aaccatcgcg
   120961 tgccagttta aacgcgggct gcacgacttt gttcagcggc atggtgccgt atttatccag
   121021 cgccagcgag aaacctgcta ccgtacccgg tgtgccggaa gccagatgcg aagtgagtga
   121081 ttttttgctg tccgggttgc cctgatcatc gaggaacata tcgcgggtcg ctttggcggg
   121141 tgccatttcg cggaaatcga tagccgtggt attgccattt ttcgagcgga ttaacataaa
   121201 accaccaccg cccagattcc ctgcctgcgg atgcgttacc gccagcgcgt agcccaccgc
   121261 cacggcggca tcaacggcat tcccgccctc cttgagaata tccaccccca cctgagtggc
   121321 agtggcgtcc acagacgcta ccattccctg tttcgcgcgt accgggtgga agacatcttc
   121381 ctccacacca tacgagacgg gcggcgcagg aggcgcggcg gcggcgctaa aacaacttcc
   121441 tgagagcaga gcagcaatgg ccacccggcg taaaaacgtc ggttttatca tcgttattct
   121501 ccagagatta aggggcaacc ccagctaagc ctggtatata actctgaatt aatcatcgtt
   121561 ttgtcgggaa gcgagtaaac ttaaaggata tcttcagagg aggatacgcg atgaaacgac
   121621 ttctgattct tacggcactc ctgccgtttg tcggctttgc acagcccatt aatactctga
   121681 acaaccctaa ccagccgggg tatcagatac ccagccagca gcggatgcaa acccagatgc
   121741 agactcagca aatccagcaa aaagggatgc tgaatcagca actgaaaacg caaactcagt
   121801 tgcaacagca gcatttagaa aaccagataa acaataattc tcagcgggtg ttgcagtcac
   121861 agccggggga gcgaaatccc gcccggcagc aaatgctgcc caacaccaac ggcgggatgt
   121921 taaacagcaa ccgtaatccg gatagttcgt tgaatcagca gcatatgctg ccggagagga
   121981 gaaacggcga catgctgaat cagcccagca cgccgcagcc tgacattccg ttgaaaacta
   122041 ttgggccgta aagttcggac caatcacgtc aatcgcatcg gtacagatgc aatccacgcc
   122101 ccagcgcagc aactctgccg cgcgctgggg tttattgacg gtataaacca gaatccgcag
   122161 tccggcgtct ttcaactgca tcactcgcac tttatcgagt aacttatgat tgagatgaat
   122221 agagacgcag cccagccgcg cggtcagttc gcgccagtcg tcgcgccact catcaagcaa
   122281 taaaccacgc ggcagttccg gtgccgccag ctgtgcagct tctaaagcat caatctcaaa
   122341 cgatgacagc agcggcggcg tcataccggc ccacagttgg cgtgccgcca gcgccaccat
   122401 tttgcccgtt aatggtccag tgccagtggt gggtttgatt tcgatattcg ccatcatccc
   122461 gtgttcgcga cagcgttccg ccacctgcga aagcaacggc agcggctcac ctttaaacgc
   122521 tttgctgtac caactgcccg catccacgcg cagtaaatcc tgccagttca attcacccgc
   122581 gacgccccag ccgttgctgg tgcgttcgag attgtcgtca tggagcagga agatctcgcc
   122641 atctttcgat aacttcgcgt caaattcgat catcttatga ccgtattttg ccccaacgtc
   122701 gattgccgcc agggtgtttt cgggggccag cttaccgccg ccacgatgag cgacgatgcg
   122761 gggataaggc cagttactca tactcgttgt cctgtttcac catcaaaaag atgcagctga
   122821 ttttccgcca gatgcagcca cagcgtgctg cctgccgtcg ggcgctcctg atgcgccagt
   122881 cgcaccacca gcttctgctc gccccagcgt ccgtgcgcca ggttatctgc gccgaggatc
   122941 tccagcgtat ccatcaccag cggtacgcca ccttctgctt gcgagcttag cgcaatatgt
   123001 tccgggcgaa tgccgagagt cattttacgc ccggcatact gacggtagcc accgtttagc
   123061 ggtagcacga taccgccgtc cagttcgaaa tgcgtgcctt cggtattcac gcggcctgtc
   123121 agcaggttca tcgccggact gccgataaaa ctcgctacaa acaggctggc gggcttttcg
   123181 tagacttcaa ctggtgtgcc aatctgttcg gcaacgccgc cgttcatcac cattactcgc
   123241 tgggcgagcg tcatcgcttc aacctgatcg tgagtaacgt agagtgaagt cgttttcagg
   123301 cgacggtgca actgttgcag ttcaagacgc atctgcacgc gcaacttggc atcgaggtta
   123361 gagagcggct catcaaacag gaacaccgcc ggatcgcgca caatcgcgcg gcccatcgcc
   123421 acacgctggc gctgaccgcc ggaaagctcg cgcgggcgac atttgagcag accgtccagc
   123481 tccagaatgc gcgccgcttc tttaacgcgc tcggcaattt gctgcttgcc catgccgcga
   123541 attttcagcc cccacgccat gttttcttcg acactcatat gcggataaag cgcgtagttc
   123601 tggaacacca tcgcaatccc gcgatctttt ggctccattt cagtcacgcg ctggtcgttg
   123661 atccagatat cgccttctgt cacccgctcc agcccggcaa ccatgcgcag cagcgtcgat
   123721 ttcccgcagc cagacggccc gaccatcacg ataaattcgc catccgccac atcaagggtc
   123781 agcggtttaa tgacctgggt tttaccatcc cagcttttgg ttactgcctg taatttcagt
   123841 cctgccatct tatttctcac tatcgaccag gccgcgcacg aaggcacgct gcatcactaa
   123901 aacaatcacc accggaggga taagcgttaa caacatcgcc gccatcactg agttccattc
   123961 cgtggtgcct tcgcctgtag cgatcatccc tttgatccct gccacggtgg tgccgagatc
   124021 cacatcggta ataatcaaca acggccacaa atactgattc cagccgtaga taaaggtgat
   124081 cacaaacagc gccgccagat tagttttgga gagcggaaaa acgatgtcgc aaaagaagcg
   124141 cattggcgat gcgccgtcga tccgcgcggc ttccaccagc tcatccggca gcgtcataaa
   124201 gaactggcgg aacaggaaag tagcgctcgc cgaggccatc agcggcagcg ttaaaccggc
   124261 gtagctgtcg agcatctgca ggttggcgat gacttccacc gtcgggaaga tacgtacttc
   124321 aaccggcagc atcagggtga taaaaatcat ccagaagaag aggttacgta gcggaaaacg
   124381 aaaccagaca atggcaaatg ccgagagcat cgagacggta attttgccga gcgtaatgct
   124441 gaacgccatc acaaagctgt taagcaacat ccgccagaac ggcgcgctat tcgtgcctac
   124501 cccgttcacc cagatgttgt ggatgttttc cagcagatgt gtgccgggga tgagcgtcat
   124561 cggcgcggca tagacggcct gtttatccag cgtcgccgcg acaaacgcca cgtacagcgg
   124621 gaagaggatc accgcgatcc cgaggatcag catggtatgg ctgaatatcg tcagccacgg
   124681 acggttctca atcattggta acgcaccttg ctttcaacat agcggaactg caccaccgtc
   124741 agcacgatga cgaggaacat caacaccacc gactgtgcgg cagacgaagc cagatccagt
   124801 ccggtaaaac cttcgcggta gatcttataa atcagcgtcg tggtggcctg aaccggcccg
   124861 ccggacgtgg cggcgtcgat caccgggaag gtgtcgaaga aggcatacac caggttcact
   124921 accagcagga agaaactcac cggggcgata agcggcagcg caatcttaaa gaagcggcga
   124981 atcggccctg caccgtcgat ggctgcggct tcgatcaacg aacggggaat ggattgcagc
   125041 gcggcataga agaacaggaa gttgtagctg atttgcttcc atactgaggc aaacaccacc
   125101 agaaacattg cctgaccgct gttttgcgcg tggttccagt cgtagccgaa ctccgcgaga
   125161 aaatgggtga tcagcccgcg accggggtta aacaggaaga tccacaatac ggcggcaacg
   125221 gcgggagcca cggcgtaagg cagcaacatt aaggtttgat agaaacggct gccgcgcacg
   125281 atgtactcca ccagcgccgc aaagaacagc gataccagca aaccgctgac ggtgacaaag
   125341 gtgctgaatt ttatcgtcgt ccagaaggag tcgagatagt agctgtcatg gaacagcgtg
   125401 acgaagttat ccaggccgac aaactggctg gagaaaccaa acggatcgac gctttgtagc
   125461 gagtaccaca acgcttcgcc cgcaggccag ataaaaaaga taacggtgat gatgagctgc
   125521 ggcgcgacca gcagataagg cagccagcgc gagcggaaca ccggacggga tgatgacatt
   125581 acggttaatt cctgaactgt gccggatgcg cttcgcttat ccggcctaca cgcgatgcca
   125641 ggtcagataa gcgaaacgca tccgacatta cactgattaa gacttcgtcg atttctcaaa
   125701 gcggcgcagc aactgatttc cacgctcaac ggcggtatcc agtgcctgct gtggtgtctt
   125761 cttaccggtc cacacgctct ccagctcttc atccacaatc acgcggatct gcggcatgtt
   125821 gcccagacgc agccctttgg tgaacggcaa cggcggctta ttcagcatct gacgcgtcgc
   125881 ggtatccgcc cctgggtttt tctcgtaaaa gccctgctca cgggtcaggt catacgctgc
   125941 tttggtgatt ggcagataac cggttttctg atgccactcg gcagcgtttt ctggcttcgc
   126001 gaggaagtcg aggaacttcg ccacgccggt atacgtttct ttatttttgc cctgcatcac
   126061 ccacaggctg gccccgccga taatggcgtt ttgcggtgca tctttcgcat cggcgtcgta
   126121 aggcatcatg cctacgccat agttaaattt ggcgtactca cgaatattag cgagagaacc
   126181 ggaagaggcg gtggtcatcg cgcaatcacc gttatagaac ttctcggtgg attcatcctt
   126241 acgaccgacg tagctgaagt cgcccttctt gttcatctcc tcgagcatgg cgatgtgttt
   126301 cacctgctcc ggcttgttga actccagcac cgcgtccgtg ccgtcaaagc cgttgttttt
   126361 gctggcaaac ggcagaccgt tccaggcgct aaagttttcc agttggatcc agccctgcca
   126421 gccgctggcg tagccgcact tcataccgga ggctttcagt ttcgcggcat agtccgccag
   126481 atcctgccag gttttcggcg gttgttccgg gtctaatcct gctttcttga aggcgtcttt
   126541 gttgtaatag agaacggggg tcgagctgtt gaatggctgg gagagtaagt ggcccgtttt
   126601 gctgtcggag tagtaaccgg aaaccgtcgg cacaaactgc gactcatcga attgaatccc
   126661 cgcctcttta aacacgtcat ataccggttt aatggctttc gatgccatca tggtggcggt
   126721 gccaacttca taaacctgca aaatagccgg agcgttgccg gtacgaaatg cggcaatccc
   126781 cgcgcttaaa ttctgttcgt agttgccttt ataggtcggt acaattttgt aatccggatt
   126841 ttcggcgtta aaacgttggg ccagagaatc cacctcttta cccagttccc cttccataga
   126901 atgccagaac ggaatggtcg tcactgcctg tgcattcccc attaacgcca gtccgagcgc
   126961 cagtgctgaa gctgtataat gtaacggttt catcgtttat ctctcttgtt gtaccgaatg
   127021 cgcgaattca cgcgttttat gctcgcgggg taacatgaca tgctcgaatt acagaaaaat
   127081 aacttttttg ttacatttgt aagatagtaa ggtgtcagaa agatgacaag gcggtgacgg
   127141 cgtgggtgag ggaaaatggg agatggggca cggataagcg gaaaatagaa ggtctgaatc
   127201 aaactctaca gattactcat cgtttcatgc cggatgcggc gtaaacgcct tatccggcct
   127261 acaagatcgt gcaaattcaa tatattgcaa ctcacccagt aggcctgata agcgcagcgc
   127321 atcaggcaat tttacatttg tcacctgtct caaaggagtc ttttgactcc ttatcaatca
   127381 acgtgttatt acccgcctaa atacgcactt ctcactgctt cgttcgccag cagcgcgtcg
   127441 ccggtatcgg agagcaccac atggccgttt tccagcacgt agccgcgatc cgccagcttt
   127501 agcgcctggt tggcgttctg ctcgacgagg aagatagtca tcccctgctc gcgcagctgc
   127561 tcgatggtgt cgaaaatttg ctggatgata atcggcgcaa gaccgagcga tggctcatca
   127621 agtagcagca aacgcgggtt gctcatcagc gcgcgaccaa tcgccagcat ttgttgctca
   127681 ccgccggaca tggtgcctgc ccgctgaaca cggcgctcat gcagacgtgg aaacagttca
   127741 tacacccact ttatgcgctc ctggaactgg tcgcgttcgg caaaaaagcc gcccatcgcc
   127801 aggttctctt ccaccgtcat ccgcgagaag acgcgacgcc cttccgggac aatcgctacc
   127861 gcttcgcgca tgattttcgc cgtctgccag tcggtaatgt ctttatcatc aaacacaatt
   127921 cgcccgctgg tagcgcgcgg atcgccgcat aacgtgccga gcaaggtggt tttccccgcc
   127981 ccgttcgcgc caatgagggt gacaatctcg ccctgattga tatgcaggct cacctcatgc
   128041 agcgcctgga ttttgccgta gtgggcgctg actttgtcaa aggacaacat gactttttcc
   128101 atcttatgcc tcacctaaat aggcacggat cacgtccggg ttattacgga tttgctccgg
   128161 cgtaccgttt gccagtggcg tcccctgatt gaccacataa attcggtctg aaatccccat
   128221 caccagcttc atatcgtgtt caatcaacaa gatagtggtg ttgtgatgat tgcgcagttc
   128281 ggcaatcagc tcatccagct ctttggtttc ttttgggtta agacctgccg ccggttcgtc
   128341 gagcattaaa atctccggct gcgtcaccat gcagcgggca atctccagac ggcgttggtc
   128401 accataggcc aggttactcg cctgacggtt ggcgtgttcc agcaaaccaa tgcgctcaag
   128461 ccaggtggcg gcgcggtcga gcgcttcgct ctgggcgcga cggaaagaag gcgttttcaa
   128521 caggccagag aacagcccgg ttttcagttg ctgatgctgc gccaccagca ggttttcaat
   128581 taccgtcatt tcacggaaca gacgcacatg ctggaaggtg cgcaccacgc ccatgcgggc
   128641 aatttgctgc cccggtaaac cttcaagatg ctgatcgcgc agtaaaatgg tgccgccggt
   128701 gggtttgtag aaaccggtca ggcagttaaa aaccgtggtt tttccggcac cgttagggcc
   128761 gattaacgag acgatctcct gcgggtacag ttcaagattg acgttgttca ccgccagcag
   128821 gccgccgaag cgcatcatca ggccgttcac agataataat ggctgactca tgcctgctct
   128881 cctttcgctg cgccgttttt cagcttcagt tgcgggcgcg tcatgggcag caagccctgc
   128941 ggacgccaga tcatcatcag caccatcaaa ccaccgagca ttaacatgct gtattcgttg
   129001 aaatcacgca tcaactcgcg cgacaccacc agcaaaattg ccgccagaat caccgcaaac
   129061 tgcgatccca taccgccgag caccactatc gccagtacaa acgccgattc agcaaaggtg
   129121 aaggattccg ggctgacaaa gccctggcgc gcagcaaaca gcgttccggc aaaaccggca
   129181 aacgcggcac tgatggtaaa agcggtcagc ttgatacgac gcgggcttaa gcccagcgaa
   129241 cggcaggcga tctcatcttc acgcaacgct tcccacgcac gccccagcgg catccgcagc
   129301 aggcggttaa tgacaaacag gcttagcacc accagcagca acgccaccag atacaggaag
   129361 atgacgcgat cggagggatc gtatttcagg ccaaagaaat tactgaacgt gtcccaaccg
   129421 ccttcacggg cggtacggct gaactcaagg ccgaagaaag tcggtttcgg gatctgactg
   129481 attccgttcg ggccgccggt aatttcggtg ttattgagca gcaatatgcg cacaatttcg
   129541 ccgaagccga gggtaacgat cgccagataa tcaccacgca gacgcagcac cggaaagccg
   129601 agcaggaagc ccgccgccgc tgccattaac ccggcaatcg gcagacaggt ccagaagccc
   129661 aagccgtaat agtgattgag cagcgcaaaa gtgtaagcgc cgatggcgta aaaaccgccg
   129721 taccccagca ccagcaggcc ggaaagccct accaccacgt tcagcccgag gccgaggata
   129781 atgtagatca tggtcagggt ggcgatatcc accgtcccgc gtgaaaccat aaacggccac
   129841 gccaccgcaa gcaccaacag cgccacgagg aacagtttct gcttcaccgt ggagccatca
   129901 atcgcgggca gaataaactt cggcccggaa acgcttttca accctttctg gaaagcaggt
   129961 cgcaaaatct ggaagaaaaa gaccaccgcc gtgccgataa acacccactg ccaacggaca
   130021 tccgaagccg tgtcgaccac cagtttggtg ccgtccagct ccagctgtac gcccataaag
   130081 acgccagcga ggataaagaa catcgcggca gagagcagcg ccattgcaat atgcatcggt
   130141 ttcatacttt ctctacctcc gggcgcccca gaataccggt cggcatcacc agcagtacca
   130201 ggatcagcag ggcaaatgag accacatctt tatattccgt actcagatag gcagaagaga
   130261 gcgcctccgc aatccccagg atcagcccgc caatcatcgc ccccggaatg ctgccaatcc
   130321 cgccgagcac cgccgcggta aaggctttca tcccggccat aaagccgatg taggggttaa
   130381 tcacaccgta gaactgaccg agcagcacac ccgccaccgc cgccatcgcc gcgccaatca
   130441 caaaggtcag cgcaatcacc cggtcggtgt tgatgccaag caggctcgcc attttcagat
   130501 cttccgcgca ggcacgacag gcgcgcccca tacgggaata gcgaatgaaa atcgtcagcg
   130561 ccagcatggc gaggaaggta acaatccaaa tcaccgcctg catggtagta atagaggcag
   130621 agaagttttc gctatggccc accacccact gcccgttaaa cagactgggc agcgccacgt
   130681 cacgcgaacc ttcggtcagg ctaacgtagt tttgcaggaa gatggacatg ccgattgccg
   130741 agatgagcgc aatcaggcgc ttagagttac gcaccgggcg ataggctacc cgttcgatac
   130801 tccagccgta ggcgctggca atgacgattg cgccgacgaa tcccgccgcc accagcagcc
   130861 agccggtatc aatgcccatc atcatcagcg cggcgatgat cataaatgag acgtagctgc
   130921 caatcatata aacctcgccg tgggcgaagt tgatcatgcc gataatgccg taaaccatgg
   130981 tgtagccgat ggctatcagc gcgtaggtac tgcccagcgt gacgccgtta aacatctgct
   131041 gcaagaaata caaaaactgc tcagacataa ggtaaccttt ctaaacccgc ccgcatttta
   131101 cgggcggtgg gatgatcact tggctgccgt ggatgaaccg tcggcgtgcc actggaagac
   131161 accaaaatca aatcccttaa gatcgccttt ttcatcccag ttcagcggcc caatcacggt
   131221 gtttgcaccg ttagctttta aatctttcac cagcgccagc ggctcatcgc tgccagtacg
   131281 ctcaagggca gtcgccagag attgcaccgc cgcgtaggtg atccagacat aaggcccgga
   131341 cggatctttc ttgtctgctt tcagcgcatc aacgatgccc tggtttgccg gatcctggtc
   131401 atagcgtttt ggcatagtga ccaacatgcc ttcggcagca tcaccggcaa tattcgacaa
   131461 tgatgcgtta cccacacctt ccggccccat aaactgagtt ttcaggccaa cggaacgggc
   131521 ctggcgcagc atctgcccca tttccgggta gtaaccgccg tagtaaacga agtcgatgtt
   131581 ttcttttttc aggcgggcga tcagcgcgga gaaatctttc tccccggcgg taataccgtc
   131641 gaagaagacg acgttggcgt tagccgcttt tagcccgtcc tgcaccgaac gcgccagccc
   131701 ttcgccatac tgttgtttgt cgtgaatgat ggcgatgcgc tggggcttca ccgtctcaag
   131761 aatgtatttt gccgccgtag gcccctggga agagtccagc ccggcagtac gcataatgtg
   131821 ttgataaccg cgttgggtca gctccgggtt ggtcgctccc ggcgagatca tcagaatacc
   131881 ttcgtcttcg tagatatctg acgcaggttg ggtagaagaa gaacacagat gaccaataac
   131941 gtatttaatg ccgtcgttaa cgattttgtt ggcgaccgca acggcttgtt tcgggtcgca
   132001 ggcgtcgtca tattccacgc ccaccagctt atcgccttta attccccctt tggcattaat
   132061 gtctttaatc gcctgacgcg cgccgttaaa ttccatatcg ccccactggg caatcgggcc
   132121 ggacatcgcg ccgacaacgg caactttaat atcgtcagcc atagcggtgt gtgaaattgc
   132181 cagtgcaatc atccctgcga tgatagtttt cgcattccgt ttcatagtca aaaatcccca
   132241 ttcgtgatgt tgtgttgctt tgtttttatg tgttaacaaa tcagactgtt ctttttttat
   132301 actgcactgt ttttgcctgt ctgattaagg gtttagcgca gtattttgtg ataatagtga
   132361 ttaaaatccc tatttttcag tcgattaaga acagataata ttctgaattt attgatagat
   132421 aaacagaaaa aagtgccttt gtcagcataa aataacggca caaagggcgg aataattcac
   132481 tatcattcag gggattatgc tgggtatttt tcattctata atgttttaac tttgtaatta
   132541 ttgctgttaa aaaattaatc acctgccaaa agaaataaaa aagagaaagc ctccgattaa
   132601 attatttcgc tacactggtt ccacttttgt gatttacacg ggttacccat gaagctgacc
   132661 atcattcgat tagaaaactt tagcgaccag gaccggattg acctgcaaaa gatctggccg
   132721 gagtattccc cttcctcttt acaggttgac gataaccacc gcatctacgc cgcgcgtttt
   132781 aacgagcgcc tgctagcggc cgtgcgggta accttaagcg gcaccgaggg agcactggat
   132841 tccctgcgcg tgcgggaagt cacccgccgt cgcggtgtgg ggcaatatct gctggaagag
   132901 gttttgcgta acaatcctgc cgtttcatgc tggtggatgg cggatgcagg cgtggaagat
   132961 cgcggtgtga tgacggcgtt tatgcaggcg ctggggttta cggcgcaaca gggcggctgg
   133021 gagaagcgtt aatcgtcaag ttgatttcaa aagtgatgac caacgagaaa ttgcctgatg
   133081 cgctacgctt atcaggccta caatgtgtgt tgcaatttac tgatttcttt ggatcttgta
   133141 ggccggataa ggcgtttacg ccgcatccgg catgaagcaa cgtactcgat attagcaatt
   133201 tggcggcaac ccaaagttgc cgattaatga ttacttcgca tcggtcgccg tgccgttggc
   133261 gtgccagtca aatacgccga actcaaagcc tttcagatca cctttctcat cccacgtcag
   133321 cggacccatc acggtatcca cggagttcgc tttcaggtat ttggcgatct cagccggatc
   133381 gtcagactga ttcaggcccg cctgcaaaga ttgcagcgcg gcgtaggtgg tccaaacgaa
   133441 tgcgccgctt gggtcctgtt ttttcgcttt gatggcgtca acaatgggtt tgttcgccgg
   133501 aacctgatcg tagttcttcg gcttggtcac cagcagccct tccgctgatt cgcccgcaat
   133561 gttagacagc gaaacgttag ccacaccttc cggccccata aactgagttt tcagccctgc
   133621 cgcgcgtgcc tgacgcagga tttgccccat ttccgggtga taaccgccgt agtaaacgaa
   133681 gtcgatattc tcttttttca gacgggccac cagcgttgag aaatcttttt ccccggcggt
   133741 gataccatca aagaacacca cgttagcatt gcctttcttc aggccgtcct gcaccgctcg
   133801 cgccagacct tcgccgtatt gctgtttgtc gtgaacgata gcaatacgct gcggtttcac
   133861 tttctcaaga atatatttcg ccgccgtcgg cccctggtcg gagtccaggc cggtggtgcg
   133921 caggatcagc tgatagccac gggcggtcag ctccggcgcg gttgccgctg gggtgatcat
   133981 taaaatgcct tcgtcttcgt agatgtcaga cgcaggctgc gttgatgaag aacagaggtg
   134041 accaatcaca tatttaatgc cgtcgttaac gactttgttc gccaccgcaa ccgcctgctc
   134101 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cgcccacgac
   134161 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
   134221 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
   134281 tgttgtggtg ttgtgattca gcactttatt tcgttttatg catgactacc cgtgctttag
   134341 cagcatactc tgctaaaaca taccccattt ttatgatatt ggaatagcta ttttgacagt
   134401 ttattaacaa tctgcgtggg gattgacgtt ttgccggagg gggaattgat tattgtagag
   134461 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
   134521 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
   134581 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
   134641 ccagcgcgcg cggatgatgt cctggctgcg ttcgtccaga ccttgcatcg cgtcggtcag
   134701 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
   134761 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
   134821 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgaa cgtctttgct
   134881 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
   134941 ctgcttagtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
   135001 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtgaacggc
   135061 gaaggagacc aggcgcacac ccacttccgg gttgaaacgg cgcactgctt tcatcaggcc
   135121 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
   135181 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
   135241 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
   135301 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
   135361 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
   135421 ctgtgccaga gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
   135481 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
   135541 aataccggga agagacaacg ggatctcttt ccctgctacg gaacccattg cagggaaaga
   135601 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
   135661 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
   135721 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttctgca accgccgatg
   135781 acaaacgcag taccagaatt tctgacaaaa ttaatgacag caatgcgcca gaaaatccca
   135841 gcaatgcgcc tccgtacagg aacgggcgca ggatgaaacc atccgtcgca ccaatcagtt
   135901 tctgcacgtt aatggagtca cggcgagcaa agatgctcag acgcacactg ttgccgatga
   135961 cgaggaacac tgctgccacc atcaacacgc cgatcatcgc ggaaacgcgc ccgaccagcc
   136021 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
   136081 taatctgggt gatacgatca cgcagcgtat tcagtgattc cgtcccctga aaatcgagtt
   136141 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
   136201 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
   136261 tctccacgcc ctgctcggcc tgtagctgtg ctaccacgcc tgctgcggcg tcatcgtcca
   136321 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atattgcgtc gccgcctggt
   136381 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
   136441 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
   136501 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
   136561 tcgcgcgttt tggcgcgtta cgaccgccgt cgccagagcc gccgaccgat ttacggaagc
   136621 gatccagacg cccgccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
   136681 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgcgagatc
   136741 aggttgatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
   136801 agacgtagaa tcccttccga caacgcgtcg tccaggttac cagtcggttc gtccgccagc
   136861 agtaccgcgg gcttgttcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgcca
   136921 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
   136981 gacacccggc gacgaatatc gtcaccgctg gcaccggcga taatcagcgg gattgccacg
   137041 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
   137101 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
   137161 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
   137221 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
   137281 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
   137341 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
   137401 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
   137461 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
   137521 cagcttcatg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcgatag
   137581 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
   137641 tcaactcttc catcaggtgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
   137701 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
   137761 caccagtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
   137821 caaccgctgc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
   137881 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
   137941 tcaggatcac gaacggcgtt ttaccttcaa cattcagcgg ctcatcgact ttcgccagaa
   138001 tctcgcccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
   138061 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
   138121 cgatcaacag ctgttcttcc agctcctcaa acagatcatc gtcgattttt ttaccgcgga
   138181 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
   138241 gcgcgaagaa accttctttg gtcggttttt cctgctcctg agcgatttct tccaccggct
   138301 gctcttctac cggaggaacc accatcaccg cctcttctgc cgcttcggca gccagcgcct
   138361 gtgcttccag ctcttcgtcg gtgatttctt ctttagccgc ttcttcttcc gccgcttcga
   138421 caatctctac ggtttccgct tcagcctgcc actcttctgg cgaaacctct tcggcgttga
   138481 cgtcttccgg caacggcagc tcttcacgct cgatagccac tggct
//
LOCUS       DAHBHC010000002       118859 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHBHC010000002 DAHBHC010000000
VERSION     DAHBHC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 118859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.002"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..723)
                     /gene="wzx"
                     /locus_tag="M2A35_000129"
     CDS             complement(<1..723)
                     /gene="wzx"
                     /locus_tag="M2A35_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S88 variant O45 family O-antigen flippase"
                     /protein_id="HCB7196481.1"
                     /translation="MFVNKSIIAGLLVLLFERFVQIISAIYANHLISKALPIEQFGIW
                     QYAFTFSNILMSLTWMCGSESVVPQLSRYSLRKKLIYCNTIFFARLSVSSLVATVSVI
                     LAITAESEIVRYYLLFSSVTLLAREPLMVGFAKWQSEGKLYLSGIIQIFGCIIRILTL
                     YYLFIVINFDIKFLALPWVLEGIFVAIVMYCCSICKFPRISLVRLNDIGFLLKRSFFI
                     WGGVVAYALFMKIDRFLLKGNIS"
     gene            complement(710..1708)
                     /locus_tag="M2A35_000130"
     CDS             complement(710..1708)
                     /locus_tag="M2A35_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196482.1"
                     /translation="MGYKNTVLFISPKFFNYEKEIVKELELTNDVIYWDERPSNNAIY
                     KSLLRLGCSILIKKYNSSYYKKLLRQIVNKKIDNVFILNPEAIDHEILQAIKKTVKVK
                     NEHCKFIMYLWDSVNNKPKVKRIINQFDSVFTFERDDAEKWGVTFLPLFYSPRFDNKH
                     EHSLKYDLCFIGTAHSDRVDLVNCITAEVRNIKDINAYTFFYFQNEIIFKLKNLLSGK
                     KINTDVEFEPLSQSEVVEMMKCSEIIIDIHHPRQRGLTMRTIECIGLNKKIITTNEDI
                     KKYDFYDPNMICVVKRHQVIVPENFINAPNVNYKNREQYSLQQWVKKIISPEGVRE"
     gene            complement(1710..2525)
                     /locus_tag="M2A35_000131"
     CDS             complement(1710..2525)
                     /locus_tag="M2A35_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7196483.1"
                     /translation="MKYTVVGSSGFIGSHMVTFLESMNKECFCPDRDYLYNKEIDLGT
                     VFYCAGYGDCKSDAYNVVDANVNKLKVILTQANFQKLVYISSTRVYLNSRDSAENSDL
                     KICHNDGRKLFNLTKLVAEELCLRSKKNVVVVRPSNVYGLALNSPLFLPQIIKNAINN
                     NHVDMYVSSDYEKDYVSVDDVVAVMYKLAESITSPGDIYNIASGYNVSAKSIADILIS
                     ETGCNIQWHKNDTCETFPVITMQKTNSIVPHHYKNVLNDLVDMIKEFKKSQGR"
     gene            complement(2588..3130)
                     /gene="rfbC"
                     /locus_tag="M2A35_000132"
     CDS             complement(2588..3130)
                     /gene="rfbC"
                     /locus_tag="M2A35_000132"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCB7196484.1"
                     /translation="MKVIETPLAGCVIIEPKVFGDSRGFFLEAWHQKKYHDIGITEAF
                     VQDNRSRSARNVLRGLHFQKNNPQGKLVSVISGEVFDVAVDLRPGSSSFGKHVGVILS
                     GDKNNQIYIPPGFAHGFCVLSEYADFHYKCTDFYDPTDEGGIIWNDKELNIQWPIDMP
                     IMSSKDNALISFSEFKEASL"
     gene            complement(3202..4284)
                     /gene="rfbB"
                     /locus_tag="M2A35_000133"
     CDS             complement(3202..4284)
                     /gene="rfbB"
                     /locus_tag="M2A35_000133"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCB7196485.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFSQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLETARQYWSSLDSARKATFRFHHISTDEVYGDLPHPDEVAQGSILPLFTEQTA
                     YAPSSPYSASKASSDHLVRAWLRTYGLPTMVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KSLPIYGKGDQIRDWLYVEDHARALYTVVTKGIPGETYNIGGHNEKKNLDVVHTICDL
                     LDEIVPKAVSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTSWVENIKSGAYQTWIEQNYKMR"
     gene            complement(4656..5549)
                     /gene="galF"
                     /locus_tag="M2A35_000134"
     CDS             complement(4656..5549)
                     /gene="galF"
                     /locus_tag="M2A35_000134"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCB7196486.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(5724..7118)
                     /gene="wcaM"
                     /locus_tag="M2A35_000135"
     CDS             complement(5724..7118)
                     /gene="wcaM"
                     /locus_tag="M2A35_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCB7196487.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMNNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQKSSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(7129..8349)
                     /gene="wcaL"
                     /locus_tag="M2A35_000136"
     CDS             complement(7129..8349)
                     /gene="wcaL"
                     /locus_tag="M2A35_000136"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCB7196488.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKELGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLVAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(8346..9626)
                     /gene="wcaK"
                     /locus_tag="M2A35_000137"
     CDS             complement(8346..9626)
                     /gene="wcaK"
                     /locus_tag="M2A35_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCB7196489.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAISRERQTGMQMVQSVLERIGEVK"
     gene            complement(9902..11380)
                     /gene="wzxC"
                     /locus_tag="M2A35_000138"
     CDS             complement(9902..11380)
                     /gene="wzxC"
                     /locus_tag="M2A35_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCB7196490.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(11382..12776)
                     /gene="wcaJ"
                     /locus_tag="M2A35_000139"
     CDS             complement(11382..12776)
                     /gene="wcaJ"
                     /locus_tag="M2A35_000139"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCB7196491.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSSIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(12831..14201)
                     /gene="cpsG"
                     /locus_tag="M2A35_000140"
                     /gene_synonym="manB"
     CDS             complement(12831..14201)
                     /gene="cpsG"
                     /locus_tag="M2A35_000140"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCB7196492.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FHDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMTAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(14394..15830)
                     /gene="cpsB"
                     /locus_tag="M2A35_000141"
     CDS             complement(14394..15830)
                     /gene="cpsB"
                     /locus_tag="M2A35_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCB7196493.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGELTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDAVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(15833..17056)
                     /gene="wcaI"
                     /locus_tag="M2A35_000142"
     CDS             complement(15833..17056)
                     /gene="wcaI"
                     /locus_tag="M2A35_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCB7196494.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAESVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLD
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEVHTELGQLCETFRGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(17053..17532)
                     /gene="gmm"
                     /locus_tag="M2A35_000143"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(17053..17532)
                     /gene="gmm"
                     /locus_tag="M2A35_000143"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCB7196495.1"
                     /translation="MFLRQEDFATVVCSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDGQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(17535..18500)
                     /gene="fcl"
                     /locus_tag="M2A35_000144"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(17535..18500)
                     /gene="fcl"
                     /locus_tag="M2A35_000144"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCB7196496.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(18503..19624)
                     /gene="gmd"
                     /locus_tag="M2A35_000145"
     CDS             complement(18503..19624)
                     /gene="gmd"
                     /locus_tag="M2A35_000145"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCB7196497.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(19651..20199)
                     /gene="wcaF"
                     /locus_tag="M2A35_000146"
     CDS             complement(19651..20199)
                     /gene="wcaF"
                     /locus_tag="M2A35_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCB7196498.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(20340..21086)
                     /gene="wcaE"
                     /locus_tag="M2A35_000147"
     CDS             complement(20340..21086)
                     /gene="wcaE"
                     /locus_tag="M2A35_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCB7196499.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHRDAADF
                     VRKLKVQKDNVMITGDALLDFGDGYKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(21097..22314)
                     /gene="wcaD"
                     /locus_tag="M2A35_000148"
     CDS             complement(21097..22314)
                     /gene="wcaD"
                     /locus_tag="M2A35_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCB7196500.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(22289..23506)
                     /gene="wcaC"
                     /locus_tag="M2A35_000149"
     CDS             complement(22289..23506)
                     /gene="wcaC"
                     /locus_tag="M2A35_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCB7196501.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTAGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(23503..23991)
                     /gene="wcaB"
                     /locus_tag="M2A35_000150"
     CDS             complement(23503..23991)
                     /gene="wcaB"
                     /locus_tag="M2A35_000150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCB7196502.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(23994..24833)
                     /gene="wcaA"
                     /locus_tag="M2A35_000151"
     CDS             complement(23994..24833)
                     /gene="wcaA"
                     /locus_tag="M2A35_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCB7196503.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(24926..27088)
                     /gene="wzc"
                     /locus_tag="M2A35_000152"
     CDS             complement(24926..27088)
                     /gene="wzc"
                     /locus_tag="M2A35_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCB7196504.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITTVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(27091..27534)
                     /gene="wzb"
                     /locus_tag="M2A35_000153"
     CDS             complement(27091..27534)
                     /gene="wzb"
                     /locus_tag="M2A35_000153"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCB7196505.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(27540..28679)
                     /gene="wza"
                     /locus_tag="M2A35_000154"
     CDS             complement(27540..28679)
                     /gene="wza"
                     /locus_tag="M2A35_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCB7196506.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            29338..30921
                     /locus_tag="M2A35_000155"
     CDS             29338..30921
                     /locus_tag="M2A35_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCB7196507.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(31195..33048)
                     /gene="asmA"
                     /locus_tag="M2A35_000156"
     CDS             complement(31195..33048)
                     /gene="asmA"
                     /locus_tag="M2A35_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCB7196508.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QVHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(33070..33651)
                     /gene="dcd"
                     /locus_tag="M2A35_000157"
     CDS             complement(33070..33651)
                     /gene="dcd"
                     /locus_tag="M2A35_000157"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCB7196509.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(33743..34384)
                     /gene="udk"
                     /locus_tag="M2A35_000158"
     CDS             complement(33743..34384)
                     /gene="udk"
                     /locus_tag="M2A35_000158"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCB7196510.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            34702..38019
                     /locus_tag="M2A35_000159"
     CDS             34702..38019
                     /locus_tag="M2A35_000159"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCB7196511.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISISLPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(38128..38976)
                     /gene="alkA"
                     /locus_tag="M2A35_000160"
     CDS             complement(38128..38976)
                     /gene="alkA"
                     /locus_tag="M2A35_000160"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCB7196512.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            39110..40462
                     /gene="yegD"
                     /locus_tag="M2A35_000161"
     CDS             39110..40462
                     /gene="yegD"
                     /locus_tag="M2A35_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7196513.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSTAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(40474..42420)
                     /gene="yegI"
                     /locus_tag="M2A35_000162"
     CDS             complement(40474..42420)
                     /gene="yegI"
                     /locus_tag="M2A35_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCB7196514.1"
                     /translation="MKTNIKVFTSTGELTTLGRELGKGGEGAVYDIEEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGV
                     IYFIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRRE
                     YIILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEVQQRREAFN
                     RAKMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAVTPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            42620..43081
                     /locus_tag="M2A35_000163"
     CDS             42620..43081
                     /locus_tag="M2A35_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HCB7196515.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(43146..43907)
                     /gene="pphC"
                     /locus_tag="M2A35_000164"
     CDS             complement(43146..43907)
                     /gene="pphC"
                     /locus_tag="M2A35_000164"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCB7196516.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQIAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATNMVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(43904..44563)
                     /locus_tag="M2A35_000165"
     CDS             complement(43904..44563)
                     /locus_tag="M2A35_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCB7196517.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(44784..44843)
                     /gene="ibsA"
                     /locus_tag="M2A35_000166"
     CDS             complement(44784..44843)
                     /gene="ibsA"
                     /locus_tag="M2A35_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCB7196518.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(45116..45172)
                     /locus_tag="M2A35_000167"
     CDS             complement(45116..45172)
                     /locus_tag="M2A35_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCB7196519.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            45451..46698
                     /gene="mdtA"
                     /locus_tag="M2A35_000168"
     CDS             45451..46698
                     /gene="mdtA"
                     /locus_tag="M2A35_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCB7196520.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            46698..49820
                     /gene="mdtB"
                     /locus_tag="M2A35_000169"
     CDS             46698..49820
                     /gene="mdtB"
                     /locus_tag="M2A35_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCB7196521.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMMPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIASQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            49821..52898
                     /gene="mdtC"
                     /locus_tag="M2A35_000170"
     CDS             49821..52898
                     /gene="mdtC"
                     /locus_tag="M2A35_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCB7196522.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDMRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            52899..54314
                     /locus_tag="M2A35_000171"
     CDS             52899..54314
                     /locus_tag="M2A35_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7196523.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            54311..55714
                     /gene="baeS"
                     /locus_tag="M2A35_000172"
     CDS             54311..55714
                     /gene="baeS"
                     /locus_tag="M2A35_000172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCB7196524.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            55711..56433
                     /gene="baeR"
                     /locus_tag="M2A35_000173"
     CDS             55711..56433
                     /gene="baeR"
                     /locus_tag="M2A35_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCB7196525.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            56624..56956
                     /locus_tag="M2A35_000174"
     CDS             56624..56956
                     /locus_tag="M2A35_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCB7196526.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            57103..58464
                     /gene="yegQ"
                     /locus_tag="M2A35_000175"
     CDS             57103..58464
                     /gene="yegQ"
                     /locus_tag="M2A35_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCB7196527.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(58795..59112)
                     /locus_tag="M2A35_000176"
     CDS             complement(58795..59112)
                     /locus_tag="M2A35_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196528.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            59518..60417
                     /gene="yegS"
                     /locus_tag="M2A35_000177"
     CDS             59518..60417
                     /gene="yegS"
                     /locus_tag="M2A35_000177"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCB7196529.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     SVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHDITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(60499..61272)
                     /locus_tag="M2A35_000178"
     CDS             complement(60499..61272)
                     /locus_tag="M2A35_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCB7196530.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(61378..62418)
                     /gene="gatD"
                     /locus_tag="M2A35_000179"
     CDS             complement(61378..62418)
                     /gene="gatD"
                     /locus_tag="M2A35_000179"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCB7196531.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(62466..63821)
                     /locus_tag="M2A35_000180"
     CDS             complement(62466..63821)
                     /locus_tag="M2A35_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HCB7196532.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(63825..64109)
                     /gene="gatB"
                     /locus_tag="M2A35_000181"
     CDS             complement(63825..64109)
                     /gene="gatB"
                     /locus_tag="M2A35_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCB7196533.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG"
     gene            complement(64140..64592)
                     /gene="gatA"
                     /locus_tag="M2A35_000182"
     CDS             complement(64140..64592)
                     /gene="gatA"
                     /locus_tag="M2A35_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCB7196534.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(64602..65864)
                     /gene="gatZ"
                     /locus_tag="M2A35_000183"
     CDS             complement(64602..65864)
                     /gene="gatZ"
                     /locus_tag="M2A35_000183"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCB7196535.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(65893..66747)
                     /gene="gatY"
                     /locus_tag="M2A35_000184"
     CDS             complement(65893..66747)
                     /gene="gatY"
                     /locus_tag="M2A35_000184"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCB7196536.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(67055..68107)
                     /gene="fbaB"
                     /locus_tag="M2A35_000185"
     CDS             complement(67055..68107)
                     /gene="fbaB"
                     /locus_tag="M2A35_000185"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCB7196537.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            68364..69641
                     /locus_tag="M2A35_000186"
     CDS             68364..69641
                     /locus_tag="M2A35_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HCB7196538.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            69638..70642
                     /locus_tag="M2A35_000187"
     CDS             69638..70642
                     /locus_tag="M2A35_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCB7196539.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISSAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            70639..71604
                     /locus_tag="M2A35_000188"
     CDS             70639..71604
                     /locus_tag="M2A35_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCB7196540.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(71578..72324)
                     /locus_tag="M2A35_000189"
     CDS             complement(71578..72324)
                     /locus_tag="M2A35_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCB7196541.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(72376..73194)
                     /locus_tag="M2A35_000190"
     CDS             complement(72376..73194)
                     /locus_tag="M2A35_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCB7196542.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQHRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(73259..74059)
                     /gene="thiD"
                     /locus_tag="M2A35_000191"
     CDS             complement(73259..74059)
                     /gene="thiD"
                     /locus_tag="M2A35_000191"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCB7196543.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(74056..74844)
                     /gene="thiM"
                     /locus_tag="M2A35_000192"
     CDS             complement(74056..74844)
                     /gene="thiM"
                     /locus_tag="M2A35_000192"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCB7196544.1"
                     /translation="MQIDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(74879..74975)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(75067..75339)
                     /gene="rcnR"
                     /locus_tag="M2A35_000193"
     CDS             complement(75067..75339)
                     /gene="rcnR"
                     /locus_tag="M2A35_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCB7196545.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            75460..76284
                     /gene="rcnA"
                     /locus_tag="M2A35_000194"
     CDS             75460..76284
                     /gene="rcnA"
                     /locus_tag="M2A35_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCB7196546.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            76503..76841
                     /gene="rcnB"
                     /locus_tag="M2A35_000195"
     CDS             76503..76841
                     /gene="rcnB"
                     /locus_tag="M2A35_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCB7196547.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(76923..77957)
                     /locus_tag="M2A35_000196"
     CDS             complement(76923..77957)
                     /locus_tag="M2A35_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCB7196548.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(77973..80453)
                     /locus_tag="M2A35_000197"
     CDS             complement(77973..80453)
                     /locus_tag="M2A35_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7196549.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDLAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGAGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQSLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(80469..81143)
                     /locus_tag="M2A35_000198"
     CDS             complement(80469..81143)
                     /locus_tag="M2A35_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCB7196550.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(81223..81765)
                     /locus_tag="M2A35_000199"
     CDS             complement(81223..81765)
                     /locus_tag="M2A35_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7196551.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(82058..82339)
                     /locus_tag="M2A35_000200"
     CDS             complement(82058..82339)
                     /locus_tag="M2A35_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCB7196552.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(82602..83711)
                     /gene="apbC"
                     /locus_tag="M2A35_000201"
     CDS             complement(82602..83711)
                     /gene="apbC"
                     /locus_tag="M2A35_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCB7196553.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            83843..85876
                     /gene="metG"
                     /locus_tag="M2A35_000202"
     CDS             83843..85876
                     /gene="metG"
                     /locus_tag="M2A35_000202"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCB7196554.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            86017..89811
                     /locus_tag="M2A35_000203"
     CDS             86017..89811
                     /locus_tag="M2A35_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCB7196555.1"
                     /translation="MRHFIYQDEKSHKFRAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLPQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQEQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYLKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLSLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESRADEAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTMPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            89821..93453
                     /locus_tag="M2A35_000204"
     CDS             89821..93453
                     /locus_tag="M2A35_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCB7196556.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPAQTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFVKPCDNSEAALFALRLLYEQGHSELLQTVANRWDRADMWPALEKILTQNPMEIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAHE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     GYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDTF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSCRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            93514..93831
                     /locus_tag="M2A35_000205"
     CDS             93514..93831
                     /locus_tag="M2A35_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196557.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCPELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRHECNDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            94138..95226
                     /locus_tag="M2A35_000206"
     CDS             94138..95226
                     /locus_tag="M2A35_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCB7196558.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTSEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVVSASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            95237..97516
                     /locus_tag="M2A35_000207"
     CDS             95237..97516
                     /locus_tag="M2A35_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196559.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGLADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDSPGDVLNRQREAFMTRWIGWAMQQNDGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDALAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDTGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALAMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            97509..98645
                     /locus_tag="M2A35_000208"
     CDS             97509..98645
                     /locus_tag="M2A35_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCB7196560.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTFCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLATARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            98642..100645
                     /locus_tag="M2A35_000209"
     CDS             98642..100645
                     /locus_tag="M2A35_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCB7196561.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDAIRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDLFASEEG
                     KTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQSGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSVSVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNCVLLCEQLFPMLQQQG"
     gene            100770..101231
                     /locus_tag="M2A35_000210"
     CDS             100770..101231
                     /locus_tag="M2A35_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCB7196562.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(101272..101742)
                     /locus_tag="M2A35_000211"
     CDS             complement(101272..101742)
                     /locus_tag="M2A35_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCB7196563.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(101789..102508)
                     /gene="yehT"
                     /locus_tag="M2A35_000212"
     CDS             complement(101789..102508)
                     /gene="yehT"
                     /locus_tag="M2A35_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCB7196564.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(102505..104190)
                     /gene="btsS"
                     /locus_tag="M2A35_000213"
     CDS             complement(102505..104190)
                     /gene="btsS"
                     /locus_tag="M2A35_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCB7196565.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            104412..105143
                     /gene="mlrA"
                     /locus_tag="M2A35_000214"
     CDS             104412..105143
                     /gene="mlrA"
                     /locus_tag="M2A35_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCB7196566.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     KGKDTLVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            105203..105310
                     /locus_tag="M2A35_000215"
     CDS             105203..105310
                     /locus_tag="M2A35_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCB7196567.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(105291..106022)
                     /gene="yehW"
                     /locus_tag="M2A35_000216"
     CDS             complement(105291..106022)
                     /gene="yehW"
                     /locus_tag="M2A35_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCB7196568.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(106027..106953)
                     /gene="yehX"
                     /locus_tag="M2A35_000217"
     CDS             complement(106027..106953)
                     /gene="yehX"
                     /locus_tag="M2A35_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCB7196569.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLEEA"
     gene            complement(106946..108103)
                     /gene="yehY"
                     /locus_tag="M2A35_000218"
     CDS             complement(106946..108103)
                     /gene="yehY"
                     /locus_tag="M2A35_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCB7196570.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(108110..109027)
                     /gene="osmF"
                     /locus_tag="M2A35_000219"
     CDS             complement(108110..109027)
                     /gene="osmF"
                     /locus_tag="M2A35_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCB7196571.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(109279..111576)
                     /gene="bglX"
                     /locus_tag="M2A35_000220"
     CDS             complement(109279..111576)
                     /gene="bglX"
                     /locus_tag="M2A35_000220"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCB7196572.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            111772..113487
                     /gene="dld"
                     /locus_tag="M2A35_000221"
     CDS             111772..113487
                     /gene="dld"
                     /locus_tag="M2A35_000221"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCB7196573.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(113525..114457)
                     /gene="pbpG"
                     /locus_tag="M2A35_000222"
     CDS             complement(113525..114457)
                     /gene="pbpG"
                     /locus_tag="M2A35_000222"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCB7196574.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(114631..115218)
                     /locus_tag="M2A35_000223"
     CDS             complement(114631..115218)
                     /locus_tag="M2A35_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCB7196575.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            115388..115966
                     /locus_tag="M2A35_000224"
     CDS             115388..115966
                     /locus_tag="M2A35_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7196576.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(116096..116857)
                     /locus_tag="M2A35_000225"
     CDS             complement(116096..116857)
                     /locus_tag="M2A35_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7196577.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDIA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(116910..118346)
                     /gene="mdtQ"
                     /locus_tag="M2A35_000226"
     CDS             complement(116910..118346)
                     /gene="mdtQ"
                     /locus_tag="M2A35_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCB7196578.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            118570..118653
                     /gene="yohP"
                     /locus_tag="M2A35_000227"
     CDS             118570..118653
                     /gene="yohP"
                     /locus_tag="M2A35_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCB7196579.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT        28987 a        31187 c        29362 g        29323 t
ORIGIN      
        1 tgaaatgtta cctttcagta agaatctgtc aattttcata aataatgcat aggctacaac
       61 ccctccccag atgaaaaaac tgcgttttaa caggaagcct atatcattta gtctaacaag
      121 acttattctg gggaacttac aaattgaaca acagtacatg actatcgcaa caaaaatgcc
      181 ctccagtacc catggcaatg caaggaactt gatatcgaaa tttatcacta taaataagta
      241 atatagtgtt agtatcctga taatgcagcc aaagatctgg atgatacctg agaggtataa
      301 tttcccttca gattgccatt ttgcaaagcc taccatcaat ggctctcgtg ccaagagagt
      361 aacggacgaa aatagcaagt aataccttac aatctcactt tcagctgtaa ttgcgaggat
      421 tacactgaca gtagcgacta atgatgatac agataatctt gcaaaaaaaa ttgtattaca
      481 ataaatcaat ttcttgcgta atgaatagcg cgataactga gggacaactg attctgaccc
      541 gcacatccaa gttagtgaca ttaaaatatt tgaaaaagta aatgcatatt gccaaatgcc
      601 aaactgctca attggaagcg cttttgagat aagatggtta gcataaatag cactaataat
      661 ctgtacaaat cgctcaaaca acaggacaag caatcccgca ataattgatt tattcacgaa
      721 caccttccgg tgagattatt tttttcaccc attgctgtaa agaatattgt tctctatttt
      781 tgtaattaac attcggtgcg ttaataaaat tctcaggtac tatgacctga tgtcttttta
      841 ctacgcagat catattaggg tcataaaaat cgtacttttt tatgtcttca ttcgtggtaa
      901 taattttttt atttaacccg atgcattcta ttgttcgcat tgtcagaccg cgctgccttg
      961 gatgatgaat atcgatgata atttcagagc acttcatcat ttcaacaact tcagattgtg
     1021 ataatggttc aaattctacg tctgtattta ttttttttcc gcttaataaa ttcttcagtt
     1081 taaaaataat ttcattctga aaataaaaga atgtataagc attaatgtct ttaatatttc
     1141 ttacttcagc agtaatgcaa ttgaccaaat caacgcgatc tgaatgagct gtaccgataa
     1201 aacaaagatc gtattttagc gaatgctcat gcttattgtc aaaacgcggc gaataaaaca
     1261 gcggaagaaa tgttactccc catttttcag catcatcacg ttcaaaggtg aaaaccgaat
     1321 caaactgatt tattattctt ttaactttgg gtttgttatt gaccgaatcc cacaaataca
     1381 taatgaattt acagtgttca ttttttactt taacggtttt ttttatggcc tgtaaaattt
     1441 catgatcaat tgcttcagga tttaaaataa atacattatc gatttttttg ttgacaattt
     1501 gccgtagtaa ttttttataa taacttgagt tatacttttt aatcaaaata ctacaaccta
     1561 accttagcaa tgatttataa atagcattat tgctcggccg ctcatcccaa tagataacgt
     1621 cattagttaa ttctaattct ttgactattt ctttttcata attaaaaaac ttgggagaaa
     1681 taaacaatac tgtatttttg taccccattt tatcttccct gagatttttt gaattctttt
     1741 atcatatcca ctaaatcatt caatacattt ttataatgat gtgggactat tgagttagtt
     1801 ttttgcatcg tgataacagg aaatgtttcg catgtatcat tcttgtgcca ctgtatatta
     1861 caacctgttt cagatattaa aatgtcagcg atggattttg ctgaaacatt atatccagat
     1921 gcaatgttat aaatatctcc gggtgaagtt atagattctg ctagcttata catgacggca
     1981 accacatcat ctaccgatac ataatccttc tcgtagtcag agctaacata catatcgaca
     2041 tgattgttat tgattgcatt ttttattatt tgcggaagga ataacgggct atttagtgcg
     2101 agtccataaa catttgatgg tcggacgacc acaacgttct ttttacttct tagacataac
     2161 tcttcagcaa ccaattttgt caaattgaaa agcttgcgac catcattgtg acaaattttt
     2221 agatctgaat tttctgcaga atctctggag ttcaaatata cacgagtgga agaaatgtag
     2281 actaatttct ggaaattagc ttgtgtaagt attactttta gcttattgac atttgcatcc
     2341 acaacattat aggcatcact tttgcaatcg ccatagcctg cgcaataaaa gacggtacca
     2401 aggtcaattt ctttgttata aagatagtct ctgtcagggc agaaacattc tttattcatt
     2461 gattctaaaa atgttaccat atgtgaacca atgaaacctg aactaccaac gactgtatac
     2521 ttcataatac tatagccttt tgctctatgt attaataatg acaggaagct aatttaaact
     2581 gttatgctta taagctagct tctttaaatt ctgaaaaact aattaacgcg ttgtctttag
     2641 atgacataat tggcatgtct atcggccatt gtatattcag ttctttgtca ttccagatta
     2701 tccccccttc atcggtagga tcatagaagt ctgtgcattt gtaatggaaa tccgcatact
     2761 cacttaatac acaaaaacca tgtgcaaatc caggtgggat atagatttga ttatttttat
     2821 ccccagaaag tataacacct acatgctttc caaaagacga agatccaggt cttaaatcaa
     2881 cagccacatc aaaaacttct ccactgatga cacttactaa tttaccttga ggattatttt
     2941 tttgaaaatg taaacctcga agcacatttc ttgctgagcg tgatcgatta tcttgtacaa
     3001 atgcctcagt tattcctatg tcgtgatatt tcttctgatg ccatgcttcc aaaaagaaac
     3061 cacgagagtc accaaagact tttggctcaa tgattacaca accagcaagt ggagtctcaa
     3121 ttaccttcat aattataaac cttgtattat attttttaaa tttgctaatt tttgatacaa
     3181 tttagctacc tttatcagtt actaacgcat cttgtagttc tgctcaatcc aggtttggta
     3241 cgccccactt ttgatatttt caacccatga ggtgtttgcc agataccatt caaccgtttt
     3301 gcgaatccca ctttcgaacg tctcttgcgg tttccaaccg agctcacggc tgattttatc
     3361 cgcatcgatc gcgtatcgac ggtcatggcc tggacgatcg gcaacatagg tgatttgttc
     3421 acggtatgac actgctttag gtacgatctc atccagcagg tcgcagatgg tatgtacaac
     3481 atcgagattt ttcttctcgt tatggccacc gatgttgtag gtctcaccag ggattccttt
     3541 cgtcaccacg gtatataacg cacgggcatg gtcttcaaca tagagccagt cgcgaatctg
     3601 atcgccttta ccataaatgg gtaacgattt cccttcgaga gcattgagaa taaccaaggg
     3661 aatcaacttt tcagggaaat ggtatgggcc atagttattc gaacagttgg tcaccattgt
     3721 tggaagaccg tatgtacgca gccaggcacg aacgagatgg tcgctggagg cttttgaagc
     3781 cgaataagga ctactcggcg cataggcggt ttgttccgtg aagagaggaa gaatactccc
     3841 ctgcgcaacc tcatccggat gcggtaaatc cccataaact tcatcagtag agatgtgatg
     3901 gaagcggaat gtcgcctttc gcgcactatc cagtgaggac cagtattgac gggctgtttc
     3961 taaaagaaca taagtaccca caatattcgt ttcaataaat gcagcagggc ctgtaattga
     4021 acggtcaaca tggctttcag cagccaggtg catcactgca tccggctgat gctgagaaaa
     4081 aattcgcgcc attgcagcag catcgcaaat atccgcatgt tcaaaaacat agcgttcaga
     4141 atcagaaaca tcagcaagtg attccaggtt tccggcgtac gttaatttat cgacattaac
     4201 aacactatcc tgcgtattat ttataatgtg acgaactaca gcagaaccaa taaatcctgc
     4261 gccaccagta acaagtatct tcacttattt attccatata acttcagagc atgctgtgaa
     4321 ataagcggct cttaggttga ttaatagagg tgttaatgca cgctaccgcc cctggcttta
     4381 cagctaccag agcactacat gcatgtctac gatgtgacga gcgttaccca ctcgcgctaa
     4441 acccgaaaaa ttcaaacgct aattgtctta ccaatccgct ctggaaacaa ggaaaatcct
     4501 ggaaaacttt gactaaaatc ctattgctaa ctcgttgtta ttctgattgt ttatataaaa
     4561 caacggcagg aatattcgca acaaattact ttcgccacga atcttcactg tcgttataat
     4621 tttcttatca accgttacat ccggtcagat tttcattatt cgcttaacag cttctcaatc
     4681 cctttacgga acttcgcccc ttctttgagg ttgcgtagtc catacttcac aaatgcctgc
     4741 atataaccca tttttttacc gcagtcgtaa ctgtccccag tcatcagcat ggcatcaacg
     4801 gactgttttt tcgccagctc ggcaatagca tcagtcagct gaatacgtcc ccatgcacca
     4861 ggctgagtac gttccagttc cggccaaata tcggcagaaa gcacatagcg accaacggcc
     4921 atgatgtctg agtccagcgt ctgcggctga tccggttttt cgataaattc gacgatgcgg
     4981 ctgactttac cttcacgatc cagtggttct ttggtctgaa tgacggagta ttcagagagg
     5041 tcacccggca tacgttttgc cagcacctgg ctgcggcccg tttcattgaa gcgcgcaatc
     5101 atggcagcaa ggttgtagcg cagcgggtcg gcactggcgt cgtcgatcac aacgtccggc
     5161 aataccacaa caaatgggtt gtcaccaatg gcgggtcgtg cacacaaaat ggagtggcct
     5221 aaacctaaag gttcgccctg acgcacgttc ataatggtca cgcccggcgg acagatggac
     5281 tgtacttccg ccagcagctg acgcttcacg cgcagttcaa ggagagattc taattcataa
     5341 gaggtgtcga agtggttttc gaccgcgttt ttggacgcgt gagttaccag gaggatttct
     5401 ttgatccctg cagccacaat ctcgtcaaca atgtactgaa tcattggctt gtcgacgatc
     5461 ggtagcatct ctttgggaat cgccttagta gcaggcaaca tatgcatccc tagacccgct
     5521 acaggaataa ctgcttttaa attcgtcatt atttcatcca cctgtaaaat ggttgctgaa
     5581 ttatagcttg ttcgattttt ttcgccagca tcaattaccc tgaattgatt actgaattac
     5641 ttgtgatgtt acgccgcttc gttgtggatt gcagtagcat tgttcctaag tatggctcca
     5701 tttttccagg aatggtcgca aatctactcc ctccgttccg gcaatctaaa gttaatcttt
     5761 tccacattaa caatatggtg atttatcctg tcgatatcga cggagctttg ccctctttca
     5821 ttcaccgcat gaacatttgc aagagatagc agtgtttctt ttttcgccat aaagacgccg
     5881 cgaacgtctt tgcgcatgtc aaagttcatg ctcaatgctg gtccaacaga ggatttctgc
     5941 atcaccttga tattacgcat aaaaaggtgt tgcggtttat tgtgtagctc aagcgaggca
     6001 cgcttcatct caatgttagt cagtgccaca aaggagacag cattcccggc ggagatttgg
     6061 atgccgcgca atttataagc aaggtgagtg ttatccagtt gaatattatt cactcggaaa
     6121 ttttgcggta tcgagagata tttgccttta attaccccat agccaattaa catcccggcg
     6181 ctattaatca tttcaatatt atcaatcacg aaattgtcac aaccgtaaat agcgactgtg
     6241 gcgttatcaa tgcctgcttt cttactgaaa tccggcgtga tattgcgggc tttgatatta
     6301 cgaataacaa aatgtttacc attttcaaca tgtatcaact gccgacaatc cgatcccgtg
     6361 atattcgcca cgacaaagtt tttcactgcc tgatcttccg ggtaattatt atcgtaagtg
     6421 cttcccgcaa ggcctatgcc aatgccccag ttgattttgc cgttagtaca gttgatgcgc
     6481 tcgatgacat ggtcggagat caaaatatca ctgtcgttaa ttgccacgtt ccattcaatg
     6541 gcgtcgccct gtaagtcgct gaacttacag ttggtgatgt tggcaccgat tatctggtta
     6601 tgaaatccct ggcgtaagat ggcgtaatta gcgtggctaa cggtcaggtt atcgatggtc
     6661 aggttgcgca tgacccgttt gtttttgccg ccgatataaa tctgcgttac cggaccaaag
     6721 ccgctcatcg ccagcccttt gatggtgcag tcagaaccac gcacatccag ggtgatgtta
     6781 ttcatactgc cgccctcctc ccctgtcacc tggctgccgt cctgtaagac aaatcgccct
     6841 ctgccgttgc cgcgcaggct tcccaggatg tgtaacgttt tgccaggggg gatgaagatg
     6901 ccagtgttga tattgtcgca aaccaatccg ccaggcacga cgaccgtttg cccttcgctg
     6961 aaggcttgtt taaatgaggc gatccagtcg tgtggattgt agtcgttgat attgacgctt
     7021 tgtcgggcag gaagtgcgcg agcgaaaggg gtatggagga aggcaagcgc cgagcttgcc
     7081 gtcaggaagg tgcgtcggga gagtttttta aatggcatgc gttctcctct ataaagcctg
     7141 cagcaagctg gcgagttctc gattgatcac ctgctggtta aaatcgtgtt cgactttttc
     7201 gcgcgcacgt ttgacgaccg gagccagttc gtcggtgtcc agttggctaa acgccaccag
     7261 tcgttgcgcc agtgcacgag catcgttctc aggcaccagc cagccggatt tgtcggcctc
     7321 caccagttcc ggtattccgc tatgcagagt ggaaactact ggaatgccga ccgccatcgc
     7381 ttccatgagc gctaccggaa tgccttccat atcaccatcc gcgcccgtta ccgatggcaa
     7441 caggaagaca tccgcgtcgt cgagcatcgc tttcacttca tggctcggtt taaagccagg
     7501 catctctacc acatcttcca gttgatattg ttcgatgagc gtgcgcaggc gtcgttccca
     7561 cgggccaatg ccgaggatgc gatagcgaaa tgccacgccc agctctttca actgacggca
     7621 ggcttcgatc gccacatgca ggcctttttt ctcggttaag cgtgcgacag atatgatttc
     7681 cagcggcgtt gcgggcgctt tcaccggacg cgggctaaag cgcgtcatgt ccacgcccat
     7741 acgcgatacg gcgatttttt ccctcgggca gcccattttt tgcagccttc cggcccacaa
     7801 atcgcttatc ggtaacatca ggtcgccacg gcgaaacagt tgttgatatt cgggagtgta
     7861 gtggttgagc acttcccgac tggaaatatc aatgccgtgg aaaatggtgg caattttgcc
     7921 gcgaatgaca cccagttcgc ggagttttgc tgcggttacc cccgctggac caaaatgagc
     7981 gatgaacaca tcggcgcgaa acggtgttgc gacctggccg caaatggcag acaaaatcag
     8041 attccgcgac tctgcgccat agcgtttgag gttgagcgcc tgccaggtat ttttacgatg
     8101 aatgcctcgt aacgtctggc tggcacggtg gcgtagcttc gccactttgc ccgtaggttc
     8161 gtcctgtaac caacgggttc ttgcggcaag gttgtatttc gtccatgccg cgtgggtgtt
     8221 ctgggtgtcg cctttttgca gcgcgacaat ctccacctca aagcccatat caataaacgc
     8281 ggtaatttga ttgaggacga aggtttccga agacagcgga aatttcagta aaaagaagcc
     8341 gaccttcatt tcacctcccc gatgcgctca agcacagatt gcaccatctg cattcccgtc
     8401 tgacgctcac gactgatggc ttcgctaagt cgtgcgttca gcgccggaag ctggcctaag
     8461 gtatccgcaa ccatcgcttg caggctgccg tctaataaat gacggatatc aattgccatc
     8521 tccggtagtc ccagctgctg cataatcccg gcggatttat gttcatagtt gatggcaatc
     8581 gccggagtag caaagttcat tgagataatg gcggagtgca ggcgcgtacc gacggtgagt
     8641 tcacaggcac cgagaatttt gcccatttcc agatcgttga gttcatccat cactacgtga
     8701 taacgggcag gatcgctgat atgctggcgc aggttgagcg ccaccatgcg gtcgtctttg
     8761 ttatagctgt cgatgcccgt acaggtggag agcgcaatca cctgataccc ttcatcgaga
     8821 atgcgattta ccaccccggc aaaggctttt tcatacgctt gttgagtggt gccgagacgt
     8881 ttgtcgaacg gtgccagttc gcgcagggta attgccaccg ttttctgttg tgcggcaacg
     8941 tccagccagt gttgaacagc atagctggcg gtgaagtctt ctgtgtggtg atcgaccagc
     9001 cacgcggtat cgacgccatg ttccactttt gcagtggtga tattgctgcg tttcatcaga
     9061 tcaaggctga ccgattcgcg caggatcagc gcgtcacagt gaccaaaaac gtagttcgcc
     9121 agttggttaa attgctcatc ctggaagggg ccgacgctgt gaccaatcat aaacagcggc
     9181 tttttcgcca taaacgtgca aagcgcatgt tcaaactgcg gcacgccgta gagatcgaca
     9241 aaaaacgatc ctccgacctg gataatggcg tcgtaacctg acagcaggcg cacgaagtcg
     9301 gtgaatccct gggcgatggc gatattacgc agcttgccag tgtcggtgac gcgcgagagc
     9361 agtacctgat gctggtagcg gcgacggagg acttttttaa cgcgcccgac aacgcccgct
     9421 gcgctgttgt gttgtttcat ttgcaggaac agcggatcgc ccattaccgg gcggttgagc
     9481 agccaggaag aactgaccgg atagcggctc atcacatcca cttcggcgtg tggattgaga
     9541 atgttgatgg catcaagtaa gccgcgcagg atggcgctgt cgccacgatt gccgcaagtg
     9601 tggttgccca gaataagtaa tttcattttt tcctcataaa tttgatgcca ggtgaggctg
     9661 cgtttgtttt tttcgttgcc gctttttgcc tgatgcgacg ctggcgcgtc ttatcaggcc
     9721 tacaagaccc gagcacagaa ccgtaggccg gataaggcgt tcacgccgca tccggcattc
     9781 agtgcctgat gcgacgctga cgcgtcttat caggcctaca ggtcccgagc acagaaccgt
     9841 aggatggata aggcgtttta cgctgcaccc ggcaaccgtt gtcggaaccg aaaacaacaa
     9901 ctcaccccgc ccgtaaaagc attttcattt tttcgctgcg acaaaactga cgcttcactt
     9961 ccaccaccag cggatggcgc gacagcacaa tcatcaccac aaacgccagc acgcccgcgg
    10021 ctatttgcac cgccagcagc atccccagcg ccagttgccc tttcagcaca atgcccagca
    10081 cataactgac caccagcgtc ggcagcgaga gataaaacgg cagccacaaa ctcaggatgt
    10141 actggcgata actggaacca agaaccggtt taatcatcac gaagtaactc agaatggtgt
    10201 tgataatctg caccagcagg aaaccgagcg tgacgccgat tgcgcccgcc atctgcccac
    10261 caataacaat cgccggaata aacagaaatg ttttgaatac gttgaattta aagctgatat
    10321 cgacccgcgc tttcgccatc agcagcgaac caatcgggtt acccaccgaa cgcagcagac
    10381 ccaccacaca cagcaattgc agcaccggaa taatgctgtt ccacttctca ccaaagacca
    10441 gcggtacaaa gttattcgac accaccatca gcccgagcag cgccggaaag ttgataatcc
    10501 ccactaccga cagcagcttg tagaagttaa cgcgcagctt ttcggtatcg tcctgaattt
    10561 tggcgaatgc cggaaacaac acacgggtga tgatcgggtt cagcttcatc ggcggcacaa
    10621 cggccacgtt gtacgccagg ttgtatcccc ctgccacgcc cgcgccgaga atacgcgcca
    10681 gcaccagcgt tgaaaggttg gtattgagat agttgatgat gctgtccgcc gtcagccagg
    10741 caccaaagcg taagttcggt gcaacagacg ccagcgagaa atgcagacca gggcgataaa
    10801 ttttgcggcc aaagtagcca aacagcagcg ttcttaccgc actattgacc agataaccga
    10861 ggatcgcggt catcgccagc ggccagaaat gggcgctaac caccgtaaaa gtgaagcccg
    10921 ccagcaccgc gctggtttcg atcatgccga ttttgttgaa ctccagctcc ttctgcatca
    10981 acgcgcggaa ctgttgcccg tgggggatca ccacaaacgc cagcgacaat gttttaatca
    11041 acggtgccag gtccgggtta ttcagcacgt caccaatggc atcactcaac aaaaacaccg
    11101 ccacgcacac cacaagcccc agcccgacgt tcagccagta cagcgtggtg agttcaaggt
    11161 ggctgatttc ttttcgctga ataatcgagt tagcgatacc gaagtcagaa agcgtatctg
    11221 ccagcgcgat gatcaccagc gacacggtaa gcaaaccgaa ctggtggttg tcgataatcc
    11281 gcgccagcac ggtcatctgt accagcccga ggccgatgat gatcaccgta gcaatcgccg
    11341 accacttcgc gccgctgatg gttttttcac gtaagctcat atcaatatgc cgctttgtta
    11401 acgaagcctt tgaataccgt caggaaaacg attttgatat cgaaccagac gctccattcg
    11461 cggatgtact caaggtcgaa ctcgacgcgt ttttccattt tctccagtgt gtcggtttcg
    11521 ccgcgccagc cgttaatctg cgcccagccg gtaatgcccg gtttcacctt atggcgcagc
    11581 atgtagcctt caatgagctg gcgatactgt tcgttatgcg ccaccgcgtg tggacgtgga
    11641 ccgacaatcg acatcccccc ggtcagcaca ttgataaact gcggcagttc atccagcgag
    11701 gtgcggcgca gaaagttccc cactttggtg acgcgcggat cgttctgcgt cgcctgagtc
    11761 accactttgt cgttctccat cactttcatg gaacggaact tccacacttt gatcggcttg
    11821 ccatccatgc catagcgagt ctggcggaaa ataaccggcc ctggtgaact gagtttcacc
    11881 gccagcgcaa tacagcacag caccggtgag atcagcagca gaataagcgt cgccagcaca
    11941 atgtcttccg cacgttttag caggcggtta accccggaaa gcggcgtgtc atacagcggc
    12001 accaccggta cgccgttcat ctcttcgata cgggaatgaa gaatattgaa ggtaaaaaca
    12061 tcggggatca gcagcaccga acaggtggtg tccgccagtt gatggaccag ttttttcact
    12121 cgcgcgccgt cgcacatttg catcgcgata tagacgttat gaatcttgcc cgctttcgcg
    12181 tcctcgacca gctgttgcag attgcccgcc cagtcgttag aaacgccgcc cggtttcggg
    12241 tcgtggtaaa cccccaccac ttcaaaccct aaccacggct gattacggaa gctctccatc
    12301 agcatttgtc cggcggctaa atcccccgcc accgcgacca tgcgcttgtt atagccatga
    12361 ttacgcagcc agcccgcccc aatgcgaata cttgaacggc aaaccaccag tccgatgctg
    12421 gttagcccat accacgccag ccagattttc agttgcgtgt cgaaatcatt gttgaacgcc
    12481 accagtccgg cgctgaaaat cacgcttaag gtccagtttt gtagcaacag ggcaaattct
    12541 gtcgctgccc gaacaccgcg ccatgagcga taaaaatcgg tgatgccgcc cagcatctgg
    12601 aacaccacca gcgtaatcag cgccaccaac aggtgcatgt agaggaatga cagtccgctg
    12661 acttcgcaaa ccagccatag tccggcaaac atgatggtga tatctgaaaa gcgttgcacc
    12721 atagagatta acgatgcatt ggttttcgct cgctcgcgct tttttagatt tgtcatcgtt
    12781 gttcctgtta ttagcccctt acccgcagtg gggtaaggga agatccgaca ttactcgttc
    12841 agcaacgtca gcagagttcg cgttcgcgct tccatcagcg gcacatcacc gcgcgattcc
    12901 acattcaacc gcaccaccgg ttcggtgttg gaggagcgca ggttaaagcg ccagtcggca
    12961 aaggtcatgc tgatgccatc ggtgcgatcc accgccagcg cctcacggct aaaatgctgt
    13021 tccacgcggt taatcgcctc aacggggtgc gccagtttgc tgttgatctc accgcttgcc
    13081 ggaaacgctg tcatccggtc gcgcaccagt tcacccagcg tttttccttt caggcacacc
    13141 agttcggcga ccagcagcca cgggatcatg ccgctgtcgc agtaagcgaa atcatggaag
    13201 taatggtggg cgctcatttc gccaccgtag atggcgtcct ccttgcgcat acgttcttta
    13261 ataaaggcgt gtccggtttt cgacatcacc ggcgtgccgc ccgcggcagt caccacatca
    13321 acggtgttcc aggagagacg tggatcgtgg atgatcttcg cgccgggatt tttttcgagg
    13381 aacgcttctg ccagcaggcc gacaatgtag tagccctcga taaactgccc tttttcgtca
    13441 aacaggaagc agcggtcaaa atcgccatca aaggcaatgc ccatatccgc gccgtgtttg
    13501 atgaccgcat tgcgggtgtc gtcgcggcat tccggcagta gcgggttagg aataccgttg
    13561 gggaaattgc cgtccggcgt gttgtgtact ttgattaatt ccacgggcgc gccgagggct
    13621 ttaaagcggg cttcgatggc atccaccacc ggacccgccg cgccgttccc ggagttaatc
    13681 accagcttga gcggcgtgag gtttttgaca ttgatataac cgaacaggtg atcaacgtaa
    13741 gcgtcacgca ggttgatttg ctgatagcga ccgcgtttgg tttcatcgac gggagggaag
    13801 tcgttggctt cagccagacg ctggacgtcg cgcagtccgg tatcaccgct gatcgggcga
    13861 gccccttcgc gcaccagttt catgccgttg taatccatcg ggttatggct ggcggtaact
    13921 tcaatgccgc catccacacc gagatggaac gtggcgaaat agatctcttc ggtgccggac
    13981 atgccaatat ccagcacatc gacgcccgca tcctgtaaac ctttcgccag cgccagtttt
    14041 aaggtttcgc tggtgaggcg gacatcaccg cctaacacaa tggttttcgg tttgagaaat
    14101 tcgccataag cgcgaccaat gcgccaggca atatcttcat tcagttcttc gcctaatttc
    14161 ccgcgaatat cataggcttt aaagcaggtt aattttttca tgtcgttacc ctttttcagg
    14221 caatagttgg ccctgaccga aacggccaat tttgtttttg tcgttattta caccgcggtt
    14281 tcgcattcat tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa tccgaaccgt
    14341 aggtcggata aggcgttcac gccgcatccg acaaacagct cctgatgcga cgtttacacc
    14401 cgtccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
    14461 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcagtgcgt cgcccccagc
    14521 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccgtcaat ggtgactttt
    14581 gccgttcccg cgaccaccac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaag
    14641 ccttcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcacctgc gtcgatagag
    14701 tcatatttgc cccacgggcg atacacttcg cgatgcaccc tatgctcatg gcgaccatcg
    14761 gctttgatct gctcgaccac ttttttcaca tcctgcaccg cgttacggtc tgcaatcagc
    14821 accgcatctt tggtctgcac taccaccaaa tctttcaccc cgacggtggt gaccaggcca
    14881 gattcggcgt acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    14941 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc gacatcgctc
    15001 cagcccgcat ccatcggcac cacaacggca tctgccgtac gttccatgac cgcgtaatcc
    15061 accgactctt ccgggcaggc cagaaacgct tcttcatcca cgcgaataaa atcgagatcc
    15121 ggatcgacgg cgctcatcgc tttttcgcag gcatcgagga tatccgggcg atattttttc
    15181 agttcttcga gatagcgtcc ggcacggaac aggaacatac cgctgttcca gtaatattcg
    15241 ccgcttgcta cataggcctg ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    15301 tcaaaggcca ccgcatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    15361 ccggtttcag gtagatccgg cacaatgccg aaggtcacca gcttgcccgc ttcggcatat
    15421 ggcatggcat tacgcacggc ggcgcggaac gcgtcttcat cggcaatcac atgatccgcc
    15481 gccaagacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
    15541 agcgcaatgg caggtgcagt gttacgccct gccggttcga gaataatgtt ctcggtgagt
    15601 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    15661 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgagt
    15721 tcgcctttca ggcataaaaa ctgcttggga taaagtacgc gggaaagcgg ccataagcgg
    15781 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    15841 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
    15901 cacgtgccac cgtgttgtgt ttgggtagca gaagcgcctg acggatcccc gccaccagcg
    15961 cctcgaccga ttccggttct acgcaaaccg caatgccccg aaaggtttcg caaagctgcc
    16021 ccagttctgt gtggacctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    16081 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttgcaccacc agatggcaat
    16141 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcggaaag aattgcatgt
    16201 tgtgcagtcc acgctgctgc gccattttat ccagccgcgc tttgccgccg ccctgcccga
    16261 caatggcaaa aatcagcggt tcatcgcgca ggagatcggc agcttcaata acgttttcca
    16321 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggc
    16381 caagctggtt acgaagggca tcaacatcgg cgtctgcaac atgctgaaaa cgggcaattt
    16441 ccgaccagtt ggggaagaag atgacgcttt ccgccgccac gcctttttcg atggctttat
    16501 tcatcatcga acgcgaaatc gtggagacgt tatcgacgtt atgcagtccg ctacgttcga
    16561 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
    16621 gcatggcatc tacttcgtaa tcctgaatat gcagcacggt acgcgcgcca gagagtttcg
    16681 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac cacgccaata atgcgatccg
    16741 gcttccagcg acgttgcgcc atcaacggga aaaaactgct gacggcaaaa ctgccgagat
    16801 gcaacaggcg tttcagggtg ctcggctgtt ttggcacata cagcgggcag cgccacaccg
    16861 tggcggcccc ctcttctcgt ttgtagcgcc aagcggaata gttctcgccc acctgccact
    16921 gcgggtagta aggcggtgcg gtaatgacgc gcacctcatg accttgtgcc gccagccatt
    16981 ccaccatctc gccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
    17041 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    17101 gctgttagcg tgaacattgt cactggcgag cagtgcgtcc ggcgtcagcc agcggtaatc
    17161 gtcatgctgc ccatccggca gtaacagttc ttcttccgct actctgaagc gaaaaccgag
    17221 caccacatag tgagtggtga aatccgtgcc agagaagtta tcgtcataaa agtgctgcca
    17281 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    17341 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    17401 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcgccgc gactgttctc
    17461 gacaataaag tcgagagaga caagcggggt ggagcacact accgtggcaa agtcttcctg
    17521 acgtaaaaac atcattaccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    17581 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    17641 acatccagca gtttgcgcgg cgtaccatcc ggtttgctgg catcaaaaac cacccgacct
    17701 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    17761 gtgccgacgt taatgtgcga cagcatcggc tgggtgttct ccagccagac ttcatgcgcc
    17821 agctccatga catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    17881 ggtgtaccgc tgccccatac caccacgtcc ggtgcattct gtgccgtcgc ctcgtggaag
    17941 cgacgcagca atgcagggat cacatgcgaa ttactcgggt ggaagttgtc atgcgggcca
    18001 tacaggttgg tcggcatgac tgagcggtaa tcgcgtccat actggcggtt gtaagattcg
    18061 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    18121 ccctgcaata attcgctttc tgccatcggc tgttttgcca gtttcgggta gatacaggac
    18181 gatccgagaa acagcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    18241 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    18301 cccactttcg ccgccgccag atagacctgg tcaatacgtt cgctggcaaa gaaatcatgc
    18361 accgcgcggc tgtccagcag gttcagctcg tcgcgggtgc gtaataccag ttccacatca
    18421 ccgcgctgtt cgagctgccg cctgatggca gaaccgacca tcccgcgatg gccagcgata
    18481 aaaatgcgtt gtctactcat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    18541 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    18601 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
    18661 cgagcagcgt ttcaacttct gccggacgga agtaacgcgg gtcaacggcg ataatcacat
    18721 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    18781 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgctgccgcc atttccacga
    18841 actgacgtac ggagtactga acgccggtcg cgataacgaa atcttctggc tgttcctgtt
    18901 gcagcatcat ccactgcatt tttacgtagt ctttggcatg gccccagtca cgcagggaat
    18961 ccatattgcc gaggtacagg cacgattcca gcccctgggc gatgttggcg attgcgcggg
    19021 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaatac
    19081 cgttacaggc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
    19141 gtttggcgac cgcatacgga gagcgtgggt agaacggcgt ggtttctttc tgcggaattt
    19201 cctgcaccag accatacagt tcagaggtgg aagcctgata gaagcgggtt ttcttttcca
    19261 gaccgaggaa gcggatcgcc tcgagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    19321 tatattccgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    19381 catccggctg cacttcacgc aaaatgcgcg tcaggttgga ggtatcactc aggtcgccat
    19441 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    19501 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    19561 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    19621 acatgtctta ttcctctgta ttttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    19681 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    19741 tgcacccacg acggtgccgt cgccaatcgt gacgccaggg gcaacaaaaa catctgtcgc
    19801 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    19861 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    19921 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
    19981 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    20041 ttttgcgccg aataaacgta ataaaaatgc ccgccagcga tacaatactt gtggcgacca
    20101 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    20161 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggtcttcctt atgtactcta
    20221 aataattcga gttgcaggaa ggcggcaaga gagtgaatcc ccaggagctt acataagtaa
    20281 gtgactgggg tgaacgaacg catccgcagc acatgcaact tgaagtatga cgagtatatt
    20341 cagcttttgt tatataaggc tttcgtcttt gaggtagtac gctggcgtaa atgccaggat
    20401 aattcagccc aaaagccagg cacatgtaat atttgccgtt ggactttttt ggcgtcagca
    20461 cacaattcca tattattggt ggttgatacc ccacccatag aaaattcaga caccaggcca
    20521 ttgagttttt taaatgcata acctgcttta tacattttgg ctgccagcgc gtaatcagaa
    20581 gaaactttat attccaggtc ataacgccat tttttcaagc cggataccgg gaaaaatatc
    20641 gcctgatggc tggcgggcag gctgtgataa atataccaac ccggtttggc gctacgttta
    20701 attttatacc cgtcgccaaa atccagcagc gcatcgccgg tgatcatcac gttatctttt
    20761 tgcactttta acttacggac aaaatctgcg gcatcccgat gaaaaatatc gcccgaattg
    20821 agaaacaacg cgaacttgcc ttgtgccatc gcaatgcctt tgttcatggc gtcgtagata
    20881 ccgttatctg gctcgctgac aaagcgtagg ttaaagatac cattgagatt ttccagatac
    20941 tcacgggtgc cgtcgttgga accgccatcg acaacaatcc attcgaagct gatatcttcc
    21001 acctgcgcca gatgcgccag cgaggcatgt gttttgacta tcccttcgag gttacgaaac
    21061 gcgacagtga ttatgctaag caacatgttc ttattcttac ctcgtaatat ttaacgcttt
    21121 tcgcaaaata aacggacaga cgattaaaaa tgcatattcc gggctaaata tcgaaccggt
    21181 aaaaaacagc gataccggag taaaaagata taattgcacg cgaaaattgc ggttatcacc
    21241 aaaagcgttg atcatcattt ttatcacttt ccccatgtac cacagtgata aaaacaccgc
    21301 gaaccaggaa aaataaataa tcagcagata caaaccattg tctatggttt ttccgacatc
    21361 cgcaccgtta aatattccga atgatgcgac atattcataa agtgagccaa atctgactac
    21421 accgtcaata tgggtcaaag aataaccgac catcaccagc ggaccgacaa tacgataata
    21481 tgatgacgat ccttccgtac ctaaatcgcc cagacgggtg gaaatataag gaaacgcgat
    21541 taccacacca accaggaata cagccagaga aatcaatgct aacggtaact ttttcttaat
    21601 cgcctcttta ttcagatatt gaaatgccca ctccagcaaa taaaacagga taaaggtcat
    21661 aacccctgaa aacgatccgg ataatattat ccctgcgaga atcatagcat ctgttttagg
    21721 cgttttgata ccaaactgtt tgatgctgag ccaaattgag attaatgcca gagcgaaaaa
    21781 tgccggttcg aaataaagtg ctgtcgtgcg cttgccgccg aatttaatga aattcagcac
    21841 atagctgttg ctgtaaatca gatatttcga aatcgactcc ataatactgc tgccaccggt
    21901 gaggataatt tgtgccatct ccaccgccgc cagcgccacc accagcccga ccaccagata
    21961 aaagaaacgt aatatcttgc gatggttgtg tggcgaaatt gttttaaaac gaatactcca
    22021 caccatgcca ataatgatca caatatagac aaacagcata gttgaagtga cgtatttact
    22081 ggcatccagc gactgaccaa acagatagtt aaacgccgtg agtcccgcgc caatccctaa
    22141 ggcaatcatc aattttttaa cgctgatgcg ttctaaaaac agcagtaaca agacaggtaa
    22201 aaaagtgacg atggtgatgg ggaaactttc gccaagctgg gcgattttga cgttaaccag
    22261 cagataaatc agcggcagca acaggtagct acagattctg atagaagttg acatactcct
    22321 ccagcatctg ttgtccactg taggcggcgc ggctgcgttg gctgaactca gccagcgtgg
    22381 taccaaatat cgcctgcgcg atttccggtt tgcttaactg caccagttgc agcacctctt
    22441 cttcgctgac ggttttaccg ccggattttt gcaacacttc ccgcgccgca tcgctatggg
    22501 tggcaatcac cggcacgcca atcgatagcg cctcacacaa aatcagcggg tagttatcga
    22561 cgcgagaact gaataccagc gcatccatct gattgagcgc gctcatcagc ttacgtttgt
    22621 cggtttcaaa gccgtgatta accacgttgc cagcggtgaa cggcgagaac ttaccaaagg
    22681 tatgcagttc aattttgtcg cccagcgcca tcatctcgcg taccagttgc tggttagttt
    22741 tgccgtcgta acgcagatca tgcgccacca ccgcgatttt cggcttgccc tgggtttcgc
    22801 gcaccggagg caagtccgcc agaatcgctt cagttgccat atcaatgcca ttattgataa
    22861 tccggcaacg ccctggaccg tacaggctat tgaaagcgtc agccacatgc tggctggggg
    22921 aaataaactg acagcccagc gccagcatct cacggaataa ctggcgtttg cccgccacca
    22981 gctggtgcgc ccgatcaatc ttcaccggcg gatagttatt taaggtcggg catttctggc
    23041 agcctgtttt ccagccttcg caaccgtcgg taaaggcgca gcgtccggta acactccagt
    23101 ggtcgtgcag cgtccagacc agagtgacgt ccggtttatg gtttttcact ttttcgcaaa
    23161 agcgcaccac gctcttaaga tttagccagt agctgtgcag cacatgaaaa tgcaggacca
    23221 ccggacccgc tgtgcgagta atggtgcgat ataactcatt gaaattgcca aacagatcgc
    23281 gattaaacag acgaaacaga gcaatattcg ccatcgcggt catccgcggc gtatgtttga
    23341 tgacctgcgg atagttctga tggctgacgc tctctttgcc gcctttgccg taaccgtaga
    23401 caaaatgtga cgccagcccc tgttgcagcg cacgctggtg gagatctaac gccacacctg
    23461 ctgccccgcc ttccgccagt cgcacattaa attgcaaaat attcatttaa ttaccttcac
    23521 tcgcgctttt tctcccacca ccagcgcgtt gtccgggaca gagtcgagca ccacgctgcc
    23581 cgcgcccacg gtgacgttgt taccaagcgt gatatcacca agaataatga cgttggcacc
    23641 gagttcgacg ccgttgccaa tgtgtggaca tgccatgtta tcggcaccac gattgccgat
    23701 agtgacgccg tggcgaatgg taaaatcatc ccccgctacc acgtttttat tgatcacgac
    23761 ggcgtaaccg tgatggatag taaagcggcg gccaatggtc gcggcagcct ggatttcata
    23821 accgaaaaag cattcggtga taatgcgata cagcaccagc agcggggccg cccacagatt
    23881 gttgaggacg ttctttttgc gccacaccga acaaaaatga gcaacacgat aggcaagaac
    23941 catgcagcac gggcgtaaac tccagctgtt ggcgcgcaga tcttccagca tctttagcgc
    24001 ccccgaatac catcagccag acgtttgccg ttacgcaccg acagcagcgt cagcaaagtg
    24061 cgccaggtca tacgtttatt gcggatctgg tagagggtaa acagctgata ttttttgctg
    24121 gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga agtacccgga gaattttttc
    24181 ggtgacgagg tgatctgcat ttcaccgtga ttgatatgca ggatctgcgt cgcctcttct
    24241 actttccacg gttcgccgta ctccaccacc atccgcagga agatgtcgta atcctgtgcc
    24301 gctttcagtt cggtatcgaa caaacactct ttgaaacgcc atgcccaggt aaagacctgg
    24361 ttgccaatga tattgcgttt gtagaacagg cggcgtgaat acggcgattt gggatacagc
    24421 ggcaagcttg ccggttggga atagacttcg ccctggcaaa cgtagtcgtt agcgtacaaa
    24481 aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga cgctcagacg gttgggtgtc
    24541 cattcgtcat cgtcatcaat cccggtgata tattcccctt gcgccagcat aatcgcctgg
    24601 ttacgtaccg cgcacgcccc gctgttaatg tcgttgtgaa tgtaagtgat acgcggatcg
    24661 ttgagggcgg tgacgtactg ttgcagttgt tcccaggagg tggagcaatc atccacgatg
    24721 atcatctccc agttgctgta gtcctggcgc aaaaccgatt ttattgcccg aatcgccagt
    24781 tgttggcggt tccaggtcgg catatagatt gagatcagcg gattgtcttt cataggttgt
    24841 tctcccggct tatgagctgg tttgcttgcc ggatgcggcg tgaacgcctt atccggccta
    24901 cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga cttatattcg tattcgtaat
    24961 agccataatc ctgatacgcg ctggcgcggc ggaagatgga gttcagaatc acccctttca
    25021 ccggaatacc gttttgctca aagcggctca gactggtttc cacttctttc aatgtgttga
    25081 ccgcataacg cgccaccatt aacgtggttc cgacatgacg accaacaatt gccgcatcgg
    25141 tcactgccag aatcggcggc gtatcaatca acaccaggtc atagttttta ctcgcccagt
    25201 tcaccagttc ggcaaagcgt tcgctcatca acagttcaga aggatttggc ggcacctgac
    25261 cgcgcgggat caggtcaaat ttggcaatag aggtcggttt agcagcggta gtaatatcgc
    25321 cctgaccaat cagaatttcc gacaggccat taacgttatt agtgcccaac agctcgtggg
    25381 tgtagccttt gcgcatatcg cagtcgatca acaacacgcg tttattggtc tggctgatca
    25441 ccgccgccag gttggcgcag acaaaggttt taccaattga cgggctaacc ccggtcatca
    25501 tcaacacatt gttctgtgcc tgcatcatcg cgaagtgcaa actggtacgc agactacgga
    25561 tggcttcaat cgccagatcg gttggattcc ccaccgccag tagctggctc tgtttatagc
    25621 gtttaatccc tttgatggtt ttgacgctat cacgcgcttt ctgccattcc gacagcggga
    25681 tgctggcata gacgctaata ccgtgttctt ccagtacttg cgggctttca atgccgcgat
    25741 taaacaacga gcgcagcagc acacccacga tagaaagcat caggccaagg ataatcgcgc
    25801 cgaggataat cagccctttc ttcggtttca gcacgccagg ctgagtgatt gccgggtcaa
    25861 caatgcgcac atcgccgacg gtgctggcct cggtgatttt cagctcctgc tctttattca
    25921 gcagttgcat atagacctgc tgaccagact cgacatcgcg ggtcagacgg acaatctcct
    25981 gctgggtttt cggcatcgcc gttacgcgac cgttaagttt ggctttttcg tcttccagcg
    26041 cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac tttggtgtac agcttggaga
    26101 tttccgcctc tttaaaggtc agttcgttca actgcgcatc gatgttcacc atcgaatcga
    26161 gcaccgcttt tgcttccagc ggcagatcaa cagaatcttt atcctgacgg aaggcattca
    26221 gtttgttttc ggcaacatcc aagcggctac gtacttccgg taactgttgc gcgaggaagg
    26281 cgaggctttt cgacgcttcc gccgatttgc gctcaatatt ttgttcctga tagttacggg
    26341 cgatgctgtt aagaatgtcg cggatctgtt cacgatcttc accggtataa gtcaggctca
    26401 aaacgccagc gtctttgccg ttctccgtta ccgtcaggct gttttgcagt tggttgatca
    26461 tccccagcgt ggagtatttg gtgacggtaa actcactgcc cgggctggcg tgaatggctt
    26521 caaccatcag cgtgacgcct tcttttttca gcatctggcc cgcttgcccc cgggcgctaa
    26581 agccgccgtc gctgctcagg gtgtagtttt tgttgtccag cacattaagc gtaaacacct
    26641 gatccgccat ctctttcggg cggttaaagg tggtcacttt caccgtctcg ttctgacgtc
    26701 ccatcaggcg atcccagccc gcaccgaaaa tcgggaacgt gtttttgctc actgcaatat
    26761 cgaggtcgag atcgtccacc gttttaccaa gcaccagacg cgaacgaatc aactggatct
    26821 cggcgtccga tgcaggcggt ttgttggcta atgccgaacc aatgtcctgc actaacgaat
    26881 tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact ataaatcggc gtggcgaaga
    26941 aggtgtaaac cacggcacag agggcgaata cggtggtgat gccaatcacc caccagcgcg
    27001 cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc atcactgccc gttaccggag
    27061 cggcatgttg ttttactttt tctgtcattc ttatacctgc tctgcgttca atgcctgcgc
    27121 ccactggcgg gcagaccgtt caagtaatgt gtacaccgct gcaaacgttt cccggctttt
    27181 gcgatacgga tcggggattt cacattcgtt atcccagtga ccaaacagca tcactttgcc
    27241 gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg cgcttttcca tggtcaaaat
    27301 caggtcgtag ttgcgacaca gacggcggct gatttgacgg gcacagtgac cttccagaga
    27361 cagttgatgt tctgcggcga cgctgatagc ggtaggatca gcgcccttac cgaccagcgc
    27421 gccgagtcca gcggactcca ctttcagctc cgggtgataa cgttgcagta agcgttccgc
    27481 cgtcggggaa cggcaaatat tgccgacaca gacaactaag atgttgttaa acatgacgat
    27541 taccagttat gaatgtcgct ggctgtatcc gtcatgtaac ggacaccgct aatagttggc
    27601 agcaactgat tgatcagacg gttccagcgg gaaaccgggg cggtggtgac atacaccacg
    27661 tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg acgtggcatc ggacatatcc
    27721 agctggtaga tattggcaat cttgccgtta cgcccgccct cgcctttcag cggacgaatg
    27781 acaaagatgc cgctggcgtt ggaggtggtc atgtcgatgc cttcagcatt gcccagggct
    27841 tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc tctgtttctt cacttcaccc
    27901 atcacaaata ctttcagatc atcattacgt ggcacgtaga gaatatcgcc ggggtaaagc
    27961 aggcgattct ggttgaggtc gccgttttgc atcagcgcct gcaaagaaat gcgctcttca
    28021 cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg tgtcggtcag gccacctgcg
    28081 gcgttgatgg cgtcgagaat agtcagtggc acgttggtga tcgcctgttg accggattta
    28141 ttcacctgac cggagatata ggccttttgt gagcggaagg cggcgatatt aacgtccacc
    28201 tgcgggtcag cgatgtacgt cgctaagcgc ccggtaatat cactgcggat ttcagcgagc
    28261 gttttcccga ctacgtggac cttgccgata tacgggtaaa acatagtgcc gtcaggctgt
    28321 acccagttgc cggtgtcgct ggagctgcgg tactgaccgg ctggcgtggt gagttccggg
    28381 tgatcccaga cggtgacatt aagaacgtcc cccggcccga cgcgatactg gtaattcgcg
    28441 atctcacttt ccagcgtcat attggggcgc gctacattcg ggcgtgggcg taattggtca
    28501 atcaggcgcg gggtcagcgg ataaacattc accattttgt cgagatcgaa atcagcgtcc
    28561 tgctgtttga tgacgtcttt gcccatcgtc gacatattgc tgcccggaag tactgtgcaa
    28621 ccgcttatca aggttactga caccaataat ggcatcaatt tcattttgga tttcatcatt
    28681 gtttatttat cactttggca gagtaattat cctgtgcact attaatagca atgtcgccat
    28741 gcacatttac cttgcagtta attgaataaa aatttaactg gcatcagtcc taaaaaaatt
    28801 gatttcatcc gcaggctatt gacagaataa ttcagactgg tctttcaggc atccagacac
    28861 gctaccgccc ctggcttttt agctaccaat acactgattt agtttaattt ttcacaccct
    28921 ctcagcatgc agtcgttgat gagaaagggt tattacggaa attaacttcc gaatataagg
    28981 tgacattatg gtaattgaat attggctttc caataatgca ggaggaagtg ttacagctaa
    29041 cggaatagca ggcaagataa caattcggta attggctatt tttaagaatt atattaagtg
    29101 tcattcaata tggtttttag gagtttcttt aggttgacaa tatttaatat agtgtctcca
    29161 catgcgatat ttcttaaata atgttttatt attaccactt ttaattcagg gataatgtga
    29221 ggttattacc ctcaaataat atgagataaa tagtgctgca acattgcatt tttgccccga
    29281 tttatccatg atcgaattgt gacatttgtc atacaacgaa taggttttgt acttactatg
    29341 gaatggattg ccgatccgtc tatctgggcc gggttaatca cgctgattgt gatcgaactg
    29401 gtcctcggca ttgataacct ggtctttatt gccatcctcg ccgaaaaact accgccgaag
    29461 cagcgtgacc gcgcacgggt taccgggctg ctgctggcaa tgttaatgcg cctgttactg
    29521 ctggcgtcaa tctcctggct ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt
    29581 acctttagcg cccgcgactt aatcatgctg tttggtggtt tcttcctgtt gttcaaagcc
    29641 acgatggagc tgaacgaacg gctggaaggg aaagacagca ataatcccac acaacgcaaa
    29701 ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac tggacgccat tttctcactc
    29761 gactcagtga ttaccgccgt cgggatggtc gaccatttac tggtcatgat ggccgccgtg
    29821 gttatcgcta tcagcctgat gttgatggcc agcaagccgt taacgcaatt tgttaacagt
    29881 cacccgacga tcgttattct ctgcttaagc ttcctgttga tgattggctt tagcctggtg
    29941 gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt acgctgccat tggtttctcg
    30001 gtgatgatcg aggcgctcaa tcagttggct atctttaacc ggcgacgttt tctttccgct
    30061 aaccagacgc tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag cgggcaaaaa
    30121 gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg tggatcatgg taaccagcag
    30181 atctttaatc cgcaggaacg gcggatgatt gagcgggtac ttaatcttaa ccagcgtacc
    30241 gtcagcagca ttatgacgtc gcgccacgat attgagcata tcgatctcaa cgcgccggaa
    30301 gaagaaatcc gccagttgct ggagcgaaat cagcatacgc ggctggtggt taccgacggt
    30361 gatgacgcag aagatttgct cggtgttgtt cacgttatcg acctgctaca acagtctctg
    30421 cgcggcgaac cgctcaacct gcgggtgttg attcgccagc cgctggtgtt cccggaaacc
    30481 ttgccgttgt tacctgccct ggagcagttc cgtaatgccc gcacgcactt tgcttttgtg
    30541 gtggatgagt ttggctcggt ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt
    30601 gccggtaact taccgaacga agtggaagag atcgacgccc gccatgatat tcagaagaat
    30661 gcggacggtt cctggacggc gaatggtcat atgccgctgg aggatctggt gcaatatgtg
    30721 ccgctgccgc tggatgaaaa acgtgaatat cacaccattg ccgggctgtt gatggagtat
    30781 ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg gggattattt gcttaaaacg
    30841 ttgcaggtag aaagccatcg cgtgcagaag gtgcagatta taccgctgcg taaggatggc
    30901 gagatggagt acgaggtgtg atggtatcgg tcattttacc caatagccga aaggaataag
    30961 atgagggaac cgcgccatcg gcgatttacc gccgggagcc cgggttgcca gggtggtgga
    31021 gattgagcca ccctggcacg ttcaccggct tgagcgttcc atagtagcga ggaacatcaa
    31081 gtgaacggaa cagccttcga tcttacatat tcagaatata atgatgccgc cacgaacgcc
    31141 tgatgcgacg ctttacgcgt cttatcaggc ctacaggact ggtcacctgg taacctacat
    31201 cttctccagc aacttcttca catctttgcc attacgggaa tctttattcc gctcggccca
    31261 gtcattcagg cgacgtttcg cttcgtcctg tagatgtttg cgcagtaact gatccacttg
    31321 caggctgtaa ttgagttgct gccagttgcc ataaacccgc agcggtactg gcgtttcttt
    31381 caggaaatca atcagtttgc tttccccgtt ccagccaccc acgacccgaa tatcaaactg
    31441 ggtgtcgcag gtttgatctg ccagattcaa catgccttcc cctgtcagcg ccagcacagg
    31501 cgattgacct tgcatgtcgt ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt
    31561 aaagcggtca agccgcgtca cgttatcaaa gttttcagcg gccttcacat caccgccatt
    31621 acgctctacc gcttgctgaa tcatctgctg gaagttcatc ccttccatgc gcgtgtcggt
    31681 catttcgaca tgtacctgtc cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc
    31741 accggagaaa tcaccagcca gtgacatttt tccggtcaac gaaatcggat agttaaacgc
    31801 cttcagaatg gtaccaatct caacgttttc cagccgtggc tggaagttta tccgcggatt
    31861 tattgatgtc gcgtccagcg tgcccggcag tgaaacctgt ccaccgttaa gtttgccctg
    31921 cagttgagta atttccagca aacccgactt gttggtcatt tgcgtggcaa catctgtaaa
    31981 attcattccg cgccagcgca cgttactggc ctgcaacaaa atatcagccg taaagccttg
    32041 cagtccctga taggccggtt catcaatacg cgaagaaatg accgggcgcg gcaacgttga
    32101 ttggctctgc ccctgctgcg cggcaccgtt ttcaccattc gcggtttcat taagcgggat
    32161 gaggttgtca agattcagtt gcgggaattg cagcctcagc tgccattccg gtttctctgt
    32221 cagcgtaacc tgtgcttgcc cgcttagcgt actatcattg gcggtcaaac taatttggtt
    32281 aaatgaaagg cgtttatgtg actcctgcca ctgggcctgg aaactcccct gcccctgaat
    32341 accttgtttt ggtaaatcgg caccctgcaa ctgccagtta atttgttcaa tagccgccgt
    32401 taaatcatgc ggataatcag aagcatctac cgtaccgtta agggatatcg tgagatcgcg
    32461 ctgatcgcga ttaacccgcc cggagaactc aaatgaacca cgatgttggg gatcttgttc
    32521 catttgcagg cgaatattgc ggattgtcac ctgctcgtca tcttcatgct ggaacaccag
    32581 cacgctgtcc gccaccttaa gactggatat atcaaacgac catccgcgat cgtctgacag
    32641 atccggcaag gtattgtcgc gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt
    32701 ctgcggcgtc agttggatca ctgccccttt tagcatcacc tgcttaacgc tcagttgatg
    32761 actcagtagt ggtaaaagcg ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg
    32821 gcttgcgccc tgggcggtga gagacattcg cccggagagg atactaagct gcggccagac
    32881 gtgccaacgc agtggcccgt cgagctgcaa ttgataaccg ctacgtgcag caacttgctt
    32941 gaccatatag tcgcggaaat cattcggatt caccagcaac actaacgcag ataacccggc
    33001 caccagcacg accaggagta tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat
    33061 gggcctgaat tagtctttat cgattcggct ggctaccgcg ccctgctggt tgcgatattt
    33121 cgcatcttca cggcggttgt aaggtcgcgc cgccgggccg gaaagcggct caaagctcag
    33181 cgcaccaatt aacatgcccg gacgcagcgc cagcggcagc ttaccggagt tgtagaactc
    33241 cagcacaatg caaccagacc agcccggatc gatgcggtgc gcggtgacgt gcaccatcag
    33301 ccccagacgc gccagtgagg aacgcccgtc cagccagccc accagatcgg ctggcagcgt
    33361 caccgactcc agcgtcaccg ccagcgccag ctctcctggg tgaagataga acgcctcgcc
    33421 ctcgtcgaga acgatctcat cgctcatcac gcggtcaagc gcggcgctca cttcatcttt
    33481 gggaccgctc agatcgataa acgctgccgt gtgaccacgg aaggtacgaa atttattgcc
    33541 caggcgtaca tccaccgtcg cgccgttaat acgctccact ggcggacgtg ggttgatcga
    33601 caaacggcct tcatcaagcc aggcttcaat atctcggtca cacagacgca tggcattttc
    33661 tcctttcgcg catcactccc ttaacgccaa ttacgtcaag ggcataccag gttatcactg
    33721 aacggtacac aatttatcaa gcttattcaa agaactgact tattttcgct ttcaatatat
    33781 cgatcgcgat gcggtttttc ccgccgcgcg gcacgataat gtccgcatat tgtttagaag
    33841 gctcaatgaa ttgcaggaac atcgggcgca cggttttttg atattgcgcc atcactgaat
    33901 ccattgaacg cccacgctcg ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca
    33961 gcggggtatc aacgaaaatg gagaagttaa gttcgtcacg caaacgcgca tccgtcagca
    34021 acaaaatgcc ttcgagaatg atgaccttct tcggctcaac cgtcaccgtt tctttcatac
    34081 gcgtatgttc aacatagctg taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt
    34141 gtaaatgctc aagcagcaga ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa
    34201 cgcgttcttc catcgacaga tggctttgat ctttgtaata gcagtcttcg ggaattacgc
    34261 cgatgtgttc atcaccgact tgctcacgca attcacgata aagggtactg gcaataagac
    34321 tcttgccgga agccgatgcg ccagcgatac cgataatgac gcactgatga gactgatcag
    34381 tcatatattt agcgacctga ttaacctgga tgttaggaag ggggcgacga agcgccaaac
    34441 gcggcaatta tagggatttc atccgtctga taccagtcga atagcgttgc cgcgcgctca
    34501 gagttaattg ttgacaaaga attcccgggg gcaaattacg ttgatcagtt ttatgtaagg
    34561 taaaaaatgt tatacgcagt tgcgcaaatt atccgccttt acgtcacttt atgagcaatt
    34621 cgcatataaa atgtaaaact tttgtactag cataaacaca gaaacgaata ctggcgacca
    34681 ggtcttgcgg ataaagcggt aatgagcaaa caatcacagc atgtattaat tgccctgccc
    34741 cacccgctgc ttcacctggt cagtttaggt ttagtctcgt ttatctttac ccttttctcg
    34801 cttgagcttt cgcagtttgg cacccaactc gccccactgt ggttcccgac gtccatcatg
    34861 atggtggcgt tttatcgcca tgccgggcgc atgtggccgg gaattgcgct gagctgttcg
    34921 ctgggaaata tcgccgcatc catcctgctt ttttccacca gctcgctgaa catgacctgg
    34981 acgaccatca atattgttga agccgtggtc ggggcagtgc tgctacgtaa attgctgccg
    35041 tggtataacc ccttgcaaaa tctggctgac tggctgcgtc tggcactcgg cagcgccatt
    35101 gttccgcctc tgttgggggg tgttctggtt gtcctgctga cgcccggaga cgatcctctc
    35161 agggcatttt tgatatgggt actgtcagaa tccatcggcg ctctggcact ggtgccgctg
    35221 ggattgttat ttaaaccaca ctatctgctg cgccatcgca acccacggtt gctttttgag
    35281 tcgctgctca cgttagccat cacactgacg ttaagctggc tttcgatgct gtatctgccg
    35341 tggcctttta ctttcattat tgtgctgttg atgtggagcg ccgtgcgtct gccacgaatg
    35401 gaagcctttt tgatcttcct taccacggtg atgatggtgt cgctgatgat ggccgcggat
    35461 ccctccctgc ttgctacgcc gcgtacgtac ctgatgagcc atatgccgtg gctaccgttt
    35521 ttgctgatcc tgctgcccgc caacatcatg accatggtga tgtatgcctt tcgtgcggaa
    35581 cgcaaacaca tttccgaaag cgaaacccac tttcggaacg cgatggaata ttccgctatc
    35641 ggtatggcgt tagtgggcac cgagggacaa tggctgcaaa ccaacaaagc gctctgccag
    35701 tttctcgggt acagtcagga agagctgcgc ggactcacct ttcagcaact gacctggccg
    35761 gaggatctca ataaagatct ccaacaggtt gaaaagctga taagcggtga aataaacacc
    35821 tattcaatgg aaaaacgcta ctacaaccgc aatggcgatg ttgtctgggc gttgcttgcc
    35881 gtctcactgg tgcgccacac ggatggcacg ccgctctatt ttatcgctca gattgaagac
    35941 attaacgagc taaaacgcac cgaacaggtc aatcagcaac tgatggagcg catcactctg
    36001 gctaacgaag cgggcgggat tggcatctgg gagtgggagt tgaagccgaa tatttttagc
    36061 tgggataagc ggatgttcga gctgtatgaa attcctccac atatcaaacc gaactggcag
    36121 gtgtggtacg agtgcgtgct gccggaagat cgccagcatg ccgaaaaagt gattcgtgat
    36181 tcgttgcaat cacgctcgcc ctttaaactg gaatttcgca ttaccgtaaa agacggtatt
    36241 cgccatatcc gcgccctcgc caaccgggta ctgaataaag aaggcgaagt cgaacgtctc
    36301 ctcggcatca atatggatat gaccgaagtg aaacagctta acgaggcatt gtttcaggaa
    36361 aaagagcgcc tgcacattac gcttgattcc atcggtgaag ccgtggtctg tattgatatg
    36421 gcgatgaaaa ttacctttat gaatccagtg gcggagaaga tgagcggctg gacgcaggaa
    36481 gaagcgttag gtgttccgct cctgacggtg ttgcatatta cttttggcga caacggacca
    36541 ttaatggaga acatttacag tgccgacacc tcacgttccg cgatcgagca agatgtggtg
    36601 ttgcactgtc ggagcggcgg cagttacgac gtgcattaca gtattacgcc gttaagtact
    36661 ctggacggca gcaatattgg ttcggttctg gtgattcagg acgttaccga atcacgcaaa
    36721 atgctgcgcc agctgagcta cagcgcctcc catgatgcac tgacgcatct cgccaaccgc
    36781 gccagttttg agaaacaact gcgtatcctg ctgcaaacgg taaacagtac acatcagcga
    36841 catgccctgg tgtttatcga tcttgatcgc tttaaagcgg tgaatgacag cgccgggcat
    36901 gcggcgggtg acgctttgct gcgcgaactg gcgtcgttaa tgctgagtat gctgcgttcc
    36961 agcgacgtgc tggcgcgact cggtggggat gaatttggtc tgctgctacc agactgtaat
    37021 gttgaaagcg cgcgttttat cgctacacgc attatcagtg ccgtgaatga ctatcacttt
    37081 atatgggaag gccgtgtgca tcgggtaggt gccagtgccg ggattacctt gattgatgac
    37141 aacaatcatc aggcggcaga agtgatgtcg caggctgata tcgcctgtta tgcctccaaa
    37201 aatggtggcc ggggccgggt gacggtttac gaaccgcagc aagctgccgc acatagcgaa
    37261 cgggcggcga tgtcgcttga tgaacagtgg cggatgatta aagagaatca gttgatgatg
    37321 ctcgcccacg gtgtcgcttc gccacggatc ccggaagcgc gtaatttgtg gctgatttca
    37381 cttaagctct ggagttgcga aggcgagatt attgatgaac aaacatttcg tcgtagcttc
    37441 agcgatccgg cgcttagcca tgctcttgac cgccgggtat tccacgaatt tttccagcag
    37501 gccgcaaaag cggttgccag taaaggcata agcatctccc tccccctttc cgttgccggt
    37561 ttgagtagcg ccacgctggt gaatgatctg cttgagcagc tggaaaatag ccctctacca
    37621 ccacggttat tacatctgat tattccggct gaagcgattt tagatcacgc agaaagcgtg
    37681 caaaaactgc ggctggcggg atgtcggata gtgctcagcc aggtgggccg cgatctgcaa
    37741 atcttcaact cgctgaaagc gaatatggca gattacctgc tacttgatgg tgagttatgc
    37801 gccaacgtgc agggtaattt gatggatgag atgctgatta cgattattca ggggcacgct
    37861 cagcgactcg ggatgaaaac catcgccggg ccagtcgttt tacccttagt gatggatacg
    37921 ctttctggca tcggcgtcga tctgatttat ggtgaggtga ttgccgatgc ccaaccgctg
    37981 gatttgctgg tgaatagtag ttatttcgcg attaactgag gcgtgcttcc ccatcgcctg
    38041 atgcgacgct aacgcgtctt atcatgccta caaatcgctc attccccagg ccggataagg
    38101 cgctcgcacc gcatccggcg accaacgtca tgcttcgtct ggttgccagc cttccgtata
    38161 ccagatatgc aacagcgcat aagaacgcca gggcttccag cgctcggcat aacggcggat
    38221 ttgcgccggt gtcattcccg gaaatcgctg tttaatcaga taatcatccg gcaggaaaac
    38281 atctttcgcc tgccagccac gcaaagcaaa ataattcgcc gtccagcgcc cgatacccgg
    38341 aaaagtttgc agcgttttca tcgcctgctc cacatcgccc ggtattgtca ttggtaaggt
    38401 gccctccagc gccgcatttg ccagatgaat cagcgcctct gcccgtttca acggcatacc
    38461 taacgctttt aatgcctgcg ggtctgctgc tgccagccgc tgaggcgtcg ggaagcagat
    38521 atactccgga aaatcatcca gccgttcgcc ataaagctgt gccactctgg cggtcaattt
    38581 tgccgccatc gccacgctca ccagttggcc taaaatcgcc cgcacgccct gctcaaaggc
    38641 atcaacacag ccgggtaaac gcaatccggg ccgcgccgcg cctaacctgc ccaacgcacc
    38701 gttaacaatt tgtgggttac attgcagatc aaacaggcgg ctcattttcg ccagacactc
    38761 tgcggcaaca ggttctaaac ctgcacttaa atttatgtgc agagtatggc gggctatatc
    38821 cggaatagca gtcaccacgc cgcgatattc gcccaccgcc agactacggg cataataact
    38881 gtccgcgacc gtttccacac tgctcaccgc acgggcggcg agaaatccca acatccacga
    38941 ccagtcatac ggcggctgcc agttcagggt atacatcgca tctccttatt catccgcttt
    39001 cagcataaac gttattcaga cgctgcgctt tgctttcata ttccggttgt cgcgacggca
    39061 acatttcgct aaagtcacgc cccttcttca ccggcatggg gattatttcg tgtttattgg
    39121 ttttgattac ggtacagcaa actgttcagt ggcggtcatg cgtgacggta aaccgcattt
    39181 gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga
    39241 agcggtaagc gaatggctgt accgccatca tgatgttccg gcagacgacg atgaaacgca
    39301 ggcgctgtta cgtcgggcga ttcgttataa ccgcgaagaa gatatcgatg ttacggcgaa
    39361 aagcgtgcag ttcggtcttt cctcactggc acagtacatt gatgatccag aagaagtgtg
    39421 gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc
    39481 gctgtttgag gatctggtct gcgcaatgat gttgcacatt cgccagcagg cgcaggcaca
    39541 gctgccagaa gcgattactc aggcggtgat tggtcgtccg atcaacttcc aggggctggg
    39601 cggtgatgaa gcaaataccc aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg
    39661 attcagggac gtggtattcc agtacgagcc ggtcgcggct gggctggatt acgaagccac
    39721 cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc ggtggtacga ctgactgttc
    39781 attgctgctg atggggccgc agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg
    39841 tcacagtggt tgccgtattg gcggtaacga tctggatatc gcgctggcgt ttaaaaacct
    39901 gatgccgtta ctgggcatgg gtggcgaaac cgaaaaaggc atcgccctgc cgattctgcc
    39961 gtggtggaat gcggttgcca tcaacgacgt tcctgcacag agtgatttct acagtagtgc
    40021 caacggtcgt ctgcttaacg atctggtacg cgatgcccgc gaaccggaaa aagtggccct
    40081 gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg gtgcgtagcg cagaagagag
    40141 caaaatcgcc ctttcaagca ctgcggaaac ccgcgcctca ctgccgttta tcagcgatga
    40201 actggctacg ctgattagcc agcaagggct ggaaagcgcc ctcagccagc cactggcgcg
    40261 gattctggaa caggtgcaac tggcgctgga taacgcccag gaaaaaccgg acgttatcta
    40321 cctgaccggc ggtagcgccc gttcgccgct gattaaaaaa gcgctggcag aacagttgcc
    40381 gggcattccg attgcaggcg gcgatgactt tggctccgtc accgccgggc tggcacgctg
    40441 ggcagaagtg gtatttcgtt aaaagaaaat ggctcagcag cggcttaaat cagcctgcgc
    40501 ctgagccagt ttttctgccg cctgacgtaa cggttccatc aacggcatgg tccgtgctgg
    40561 agcatgaagg cggaatcgct gcaactctgt cgcgccgaca gtcaacgtcg attccagccg
    40621 atgtcgtttg gcggtcattt tcgccattac cgcttgtctg tctgctggcg ttaccgcttc
    40681 attcggcctg aacacaaaac ggcgttcgca acttgctttc cagtcgataa ctgcctgggt
    40741 cagatgatca ccaaaccctt tcacttgctt aaccccacga cgggtaacat ccgctgctgt
    40801 ttcaatacca aaggaccgta acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc
    40861 aacatcaata aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cggtgtcgtg
    40921 aagtgctgcc agagcacgtt tttcttcttc cggtaacccg agcatttcgt ccttcatttt
    40981 ttcgagcatc gtccgtttgg cgataaaacc ttccagcccg cccaactgtt ggatctggct
    41041 gactaaatgg tcatagtcca ttttcgcgcg attaaatgcc tcacggcgtt gctgaacttc
    41101 tgctttgtac gctttgcttg tcagactgcc aataatccag atagccgaca gcacaggaac
    41161 caaaataata taacgcggtt ctgcctgaag gccgcaaagc aacagcgata acgctgacag
    41221 tgcgatctca atcagaatga tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc
    41281 agtcggttgg aaatgatcgg gtaatggcaa ttgcaatgct ggcggtgcta ctgacgccat
    41341 caccatcgcc cagactttcg ccagcacaaa atcaccgctg gtggtaatga cctcttcgcc
    41401 gagatcaata aaatagataa cgccttgatt atccagcgca caccacgggc agtcggtcaa
    41461 atgagcggga taaacatgca ttgccgaaac gggacatttc tttaactgtt ggcgcagaga
    41521 atcaagtgcc gctacccacg ctttagccgt cggacgcccg gttgccacgc cactttccgt
    41581 gaatgcctgc tgaaacatgg cttcaacatc actcggtaac atcgatagcg gaatcgatcg
    41641 tggcggcggt tttaaaccac gtcgctgatt atctgacgcg taggcataac ggaaatgggt
    41701 aatatccgtc tccagcgcat tacccgcatc agagataagc ggcacaccag aataaggatg
    41761 ccggccacca aacaagacgt gaaaaatcag caacgcaagg ccaaaattat cgtgattttc
    41821 tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc tctggcggcg taaaatgcga
    41881 cacgccgact tcgcataaat gcagtgtgcc attggcgtta atctgaaagg agtcgctatc
    41941 aatcagtacc actttgctgt cgcgacctac cataaaactg ttctggttta cgtcacccac
    42001 gacgtgcccg tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg caacatagag
    42061 tagaaaatcc cacgcacaat gagggtaact ctggcgacga tgtgccgggc tatagatcat
    42121 atgaatcggt tctttaccag aaacttttgg catcataaaa ccgataactt ttccgcctcg
    42181 tccaccgtga agcgttgcct gcggccaggc gacataattc aacaactgcg cgtcagctgt
    42241 cgcagccata aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt
    42301 ggcgacgcta tcgacaaact cctcgatatc ataaaccgcg ccttcaccac ctttgcccag
    42361 ttcacggcca agagtggtca attcacctgt cgatgtaaat acttttatat ttgttttcat
    42421 aatatttcgc gggtagttat attatatttc aatgaataat aacaactaac cattaaaatt
    42481 atttctatat ggggaaaacc cactcaagaa taaaaatgat aatgcaattt tatcaggata
    42541 caattcaatt tcggcctgat aatgattaac ttccacaggt ttttgctaac tttgactaca
    42601 aagggattct ggagaaaaaa tgaaaaagat attacttatg ttgagtctgc tattttttac
    42661 taccgcgggc tttagtgaag tttcggatac gttagtgacg ggaggttatg ataaacaagc
    42721 gatgtctgac gcgattaaac atgcgcggaa agaaaccgat aaattcatcg aggttatgaa
    42781 taaaaaagat gccgatactt tcgcagtcaa agccccgata accgatcacg gaagaaccga
    42841 gcatttctgg cttacggatg tcacttattc taacggcatg tttattggcg taatcagcaa
    42901 tgaccctggt attgttacaa acgtagaata tggccaggag tggaaaatca agaaagaaga
    42961 tatttcagac tggatgtata cgcgcgggga taaaatttac ggcggttata ccattgatcc
    43021 tttattggtc acctatccaa aagaagaagc cgacgagcta agagcaaaat tagttcgcta
    43081 atcagccgtt atatttattg tttaaactgg cggttgaatt gttgtcttca accgccatga
    43141 gaaactcact ctgcccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt
    43201 accgccgggc tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc
    43261 gttgctgccg ccagtccatt gaaaaacggg gtaaagaaag gcacatgagg agaattatcc
    43321 agcatattta acgccagtcg ctggatacca tccgtaaatg ctgcaacttt gtgcgcacgc
    43381 ccagtgctgg tgaaagtttc cagtctggaa acggcatctt cgtcggtaat gaaatgcgtc
    43441 atgttggcat attcacccgc catcggcgtg agaggcaatt gcagaccgtg acccaaatct
    43501 accaccacgc cgccatcgcc aatctgcata atcaaggtgc cgtcaggcga tgaaatcagt
    43561 cccagaaatg tgcaggcaaa atcgcgaacc gctaattctt tggcttccgc ctcggcaaaa
    43621 agtcgttgac gaatggtcag taccatattt gtggcgagga catcattaag ccccaattcc
    43681 ccgccctgca ctttttgcga catataagcc atcgcttcat tgacggcgag catcgcgcct
    43741 tcgccaccct gcgagacact gcctgcgcca tcagcgacaa ataccgacaa taatggctgc
    43801 tggtcattta accaggcaat ctgcatctgg caggcatcct gacaaggcaa gtccgcgctg
    43861 atgtgcgacg ttccaacggt tgatgcatag accagacgcc agctcacact gacgtccagc
    43921 cttttggcgc ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg
    43981 aactggataa ccagctaaac agttcacgga attgtaatcc ttgcagaggc aaaggctgac
    44041 gaacgctaat ttgtgccagc gtcttcatat ccgccccctg aacaccgatg gaaaagaaag
    44101 caaacctttt atcttcttcc ccccgaaaca ctttgttggc tgcagcctgc cactcatcgg
    44161 ttggtgcacc atcggtaatc aggaaaatcc acgggcgata atacgaaata ccatttgcac
    44221 gatactcacg tttccgctcc tctaccatat ccagggcttt ggtaatggcc gcacccattg
    44281 gtgtatcgcc ctgggcaaac aggatgggcg gaaaaaaatt agcggcgctg gtaaagggtt
    44341 gttccacatg caccgggccg aacgtcacaa tgccgagttc cactcttttt aaagccagcg
    44401 gatcagcaag cagttcatca cgaaaggtaa ccaatccggc gttaagttca ttaataggtc
    44461 taccgttcat agaaccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt
    44521 tactggcaaa atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag
    44581 agaattatcg aggtccggta tcataccgac tgcaataatg aaataagagc aaacgaagaa
    44641 actttcagaa ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag
    44701 agccgtcgat tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggagag
    44761 gtttccccct ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc
    44821 agtatgatga cgtgcttcat cataaccctt tccttattaa aagccctctt ctccgggagg
    44881 ggctttcccg tttcagcatc ccgctgaaat cgtcggctta cctcctttcg ccctgaatac
    44941 agtctatcgc taacgcgtga acaaaatagt ttcctctgtg attattgcgt aacgactcgc
    45001 aaagaatatt tgctggcagg atcgcagact gcaaagcctg cggattgaca atcttatcgt
    45061 gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg tagtcttagt
    45121 aagccgggaa gcttatgact aagagcacca cgatgatgat ccacttcatc atgacccttt
    45181 ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg
    45241 tcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat aaaatagttt
    45301 catgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg attccagaca
    45361 gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt atcattccgc
    45421 gaaacgtttc aggaagagaa actcttaacg atgaaaggta gttataaatc ccgttgggta
    45481 atcgtaatcg tggtggttat cgccgccatc gccgcattct ggttctggca aggccgcaat
    45541 gactcccgga gtgcagcccc aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt
    45601 cgacgtggta tgcgttccgg cccattagcc ccggttcagg cggcgaccgc cgtagaacag
    45661 gcagttccgc gttacctcac cgggcttggc accattaccg ccgctaatac cgttacggtg
    45721 cgcagccgcg tggacggcca actgatagcg ttacatttcc aggaaggcca gcaggtcaaa
    45781 gcaggcgatt tactggcaga aattgacccc agccagttca aagttgcatt agcacaagcc
    45841 cagggccaac tggcaaaaga taaagccacg cttgccaacg cccgccgtga cctggcgcgt
    45901 tatcaacaac tggcaaaaac caatctcgtt tcccgccagg agctggatgc ccaacaggcg
    45961 ctggtcagtg aaaccgaagg caccattaag gctgatgaag caagcgttgc cagcgcgcag
    46021 ctgcaactcg actggagccg gattaccgca ccagtcgatg gtcgcgttgg tctcaagcag
    46081 gttgatgttg gtaaccaaat ctccagtggt gataccaccg ggatcgtggt gatcacccag
    46141 acgcatccta tcgatttagt ctttaccctg ccggaaagcg atatcgctac cgtagtgcag
    46201 gcgcagaaag ccggaaaacc gctggtggta gaagcctggg atcgcaccaa ctcgaagaaa
    46261 ttaagtgaag gcacgctgtt aagtctagat aaccaaatcg atgccactac cggtacgatt
    46321 aaagtgaaag cacgctttaa taatcaggat gatgcgctgt ttcccaatca gtttgttaac
    46381 gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa
    46441 atgggcaatg aaggccattt tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat
    46501 ctggtgacgc cgggcattca ggacagtcag aaagtggtga tccgtgcagg tatttctgcg
    46561 ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg aaggggcgaa agtggaagtg
    46621 gtggaagccc agagcgccac tactccggaa gagaaagcca ccagccgcga atacgcgaaa
    46681 aaaggagcac gctcctgatg caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc
    46741 tgtttattat gcgtcctgtg gccaccacgc tgctgatggt ggcgatctta ctcgccggga
    46801 ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga agtggactat ccgaccattc
    46861 aggtggtcac gctctaccca ggtgccagcc cggatgtcat gacctctgcc gttaccgcgc
    46921 cgctagaacg ccagttcggg cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg
    46981 gcggtgcgtc agttatcact ttgcagttcc agctaacatt accgctcgat gtcgccgagc
    47041 aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc
    47101 cgccggttta cagcaaagtg aacccggcag atccgccgat catgacgctc gccgtcacct
    47161 caaccgccat gccgatgacg caagtggaag atatggtgga aacccgcgtc gcgcagaaaa
    47221 tctcgcagat ttccggcgtc ggcctggtga cgctttccgg cggtcagcgt ccggctgttc
    47281 gcgtcaaact taacgctcag gcgattgccg ccctcggcct gaccagcgaa accgtgcgca
    47341 ccgccattac cggcgctaac gttaactcgg caaaaggtag cctcgacggc ccttcccgtg
    47401 cggtcacgct ttccgcgaac gaccagatgc aatccgccga agagtatcgc cagctaatca
    47461 tcgcctacca gaacggcgcg ccaattcgtc tgggcgatgt cgcaactgta gagcaaggtg
    47521 cagaaaacag ctggctcggc gcgtgggcga acaaagaaca ggccattgtg atgaatgttc
    47581 agcgccagcc cggtgctaac attatctcca ccgccgacag cattcggcag atgatgccac
    47641 agctcactga gagtctgccg aaatcggtga aggtgacagt gctttccgat cgcaccacca
    47701 atatccgcgc atccgtcgat gatactcagt ttgaattgat gatggctatc gcgctggtag
    47761 tcatgattat ctacctgttt ttgcgcaata ttccggcgac catcattccc ggtgttgctg
    47821 taccgctgtc gttaatcggc actttcgcgg ttatggtgtt tctcgatttt tcaatcaata
    47881 acctgacact gatggcgtta actatcgcca ccggattcgt ggtcgatgac gccatcgtgg
    47941 tgatcgaaaa catttcccgc tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca
    48001 agggcgcagg tgaaatcggc tttaccatta tctcgctgac cttctcactg attgcggtgt
    48061 tgatcccact gctgtttatg ggcgatatcg tcgggcgact gttccgcgaa tttgctatta
    48121 ccctggcggt agcgattttg atctcagcgg tggtgtcgct gaccctgaca ccgatgatgt
    48181 gcgcgcggat gctcagccag gagtcgttgc gtaaacagaa ccgcttctcc cgtgcctcgg
    48241 aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc
    48301 atccgtggct gaccttaagc gtggcactca gcacgctgct gcttagcgtg ctgctgtggg
    48361 tgttcattcc gaaaggtttc ttcccggtac aggacaatgg cattattcag ggcactttgc
    48421 aggcaccgca atccagctcc tttgccaata tggcccagcg acaacgccag gtcgcggacg
    48481 tgattttgca ggatccggca gtgcaaagcc tgacctcatt tgttggcgtt gatggcacta
    48541 acccgtcgct gaacagtgca cgtttacaaa tcaacctcaa accgttggat gaacgtgatg
    48601 atcgggtgca aaaagtcatc gcccgtctgc aaacggcggt agataaagtg ccgggcgtcg
    48661 atctcttcct gcaaccaacg caggatctga ctattgatac tcaggtcagc cgcacccagt
    48721 accagtttac cttgcaggcc acgtcactgg atgcgctcag tacctgggtg ccacagttga
    48781 tggaaaaact ccagcaactg ccacagcttt ctgatgtctc cagcgactgg caggacaaag
    48841 ggctggtggc gtatgtcaat gttgatcgcg acagcgccag ccgtctgggg atcagcatgg
    48901 cggatgtcga taacgccctg tacaacgcgt ttggtcagcg gctgatttcc actatttata
    48961 ctcaggccaa ccagtatcgc gtggtgctgg agcacaacac cgaaaatacc ccaggcctcg
    49021 cggcgctgga taccattcgc ctgaccagca gcgacggcgg cgtggtgccg ctaagctcaa
    49081 ttgccaaaat tgagcagcgt tttgcgccgc tctccatcaa ccatctggat cagttcccgg
    49141 taacgaccat ctcctttaac gtgccggata actattcgct gggcgatgcg gtgcaggcga
    49201 ttatggacac cgaaaagacg ctgaatctgc cggtggatat caccacgcag ttccagggca
    49261 gcaccctcgc cttccagtcg gcgctgggca gcactgtctg gctgattgtc gcggcggtgg
    49321 tggcgatgta tatcgtgctc ggcattctgt acgagagctt tattcacccg atcaccattc
    49381 tctcgacgct acccaccgca ggggttggcg cactgctggc gttgctgatt gctggtagcg
    49441 aactggatgt gattgcgatt atcggcatta ttttgctgat cggtatcgtg aagaagaacg
    49501 ccatcatgat gatcgacttc gcgctggctg ccgagcgcga gcaaggcatg tcgccgcgcg
    49561 aggcaatcta ccaggcttgt ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg
    49621 ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc
    49681 cgttaggtat cggcatggtc ggcggcctga ttgccagcca ggtgctgacg ctgttcacca
    49741 cgccggtgat ttatctgctg ttcgaccgcc tggcattgtg gaccaaaagc cgctttgccc
    49801 gtcatgaaga ggaggcgtaa gtgaagtttt ttgccctctt catttaccgt ccggtggcga
    49861 cgattttact gtcggttgcc attaccctgt gcggcatact gggcttccgt atgctgccgg
    49921 tcgccccgct gccgcaggtc gattttccgg tgattatggt cagcgcctcg ctgcccggtg
    49981 cgtcaccaga aacaatggcg tcttccgttg ccacgccgct ggagcgctca cttgggcgca
    50041 ttgccggagt cagtgaaatg acctccagca gttcgctcgg cagcacgcgt attattttgc
    50101 agtttgattt tgaccgggat atcaacggcg cagcgcgtga tgtgcaggcg gcgatcaacg
    50161 ctgcacaaag tttgctgccc agtgggatgc ccagccgccc gacctatcgc aaagcgaacc
    50221 cgtcggatgc gccaattatg atcctcacgc tgacgtccga tacttattcg cagggtgaac
    50281 tgtacgattt cgcctcgacg cagctggctc cgacgatttc gcaaatcgac ggtgttggtg
    50341 atgtcgatgt cggaggcagc tcactgcccg ccgtacgcgt cgggctgaat ccgcaggcgc
    50401 tgtttaatca gggcgtgtcg ctggacgacg tacgcaccgc cgtcagcaat gccaacgtgc
    50461 gtaaaccgca gggcgcgctg gaagatggca ctcaccgctg gcagatccag accaatgatg
    50521 agctaaaaac cgccgctgaa tatcagccgt tgattattca ctacaacaac ggcggcgcgg
    50581 ttcgtctggg cgatgtggcg acggtgaccg actcagtgca ggatatgcgc aacgccggga
    50641 tgaccaacgc caaaccggct attttactga tgatccgcaa actgccggaa gccaatatta
    50701 tccagacggt tgacagcatc cgggcaaaat taccggagtt gcaggaaacc attccggcgg
    50761 cgattgatct gcaaattgcc caggatcgct cccccaccat tcgcgcctcg ctggaagaag
    50821 tcgagcaaac gctgattatc tcggtggcgc tggtgattct ggtggtgttt ttattcctgc
    50881 gctcgggtcg cgccactatt attcccgccg tttcggtgcc ggtttcgctg attggtacgt
    50941 ttgcggcgat gtacctgtgc ggattcagtc tcaataacct ttcgttaatg gcgctcacca
    51001 tcgctactgg tttcgtggtg gatgacgcca tcgtggtgct ggaaaacatt gcacgtcatc
    51061 tggaagcggg aatgaaaccg ttgcaagccg cactgcaagg tactcgcgaa gtcggtttta
    51121 cggtgctgtc gatgagtctg tcactggtgg cggtgttcct gccgctgctg ttgatgggcg
    51181 gattgccggg ccgactgtta cgcgaatttg ccgtgacgct ttctgtcgcc attggtatat
    51241 cgttgctggt ttctctgaca ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca
    51301 agccgcgcga gcaaaagcga ctgcgtggtt ttggtcgcat gttggtagcc ctgcaacaag
    51361 gctacggcaa gtcactgaaa tgggtgctca atcatacccg tctggtgggc gtggtgctgc
    51421 ttggcaccat tgcgctgaat atctggctgt atatctcgat cccgaaaacc ttcttcccgg
    51481 agcaggacac tggcgtgttg atgggcggga ttcaggcgga tcagagtatt tcgtttcagg
    51541 cgatgcgcgg taagttgcag gatttcatga aaattatccg tgacgatccg gcagtggata
    51601 atgtcaccgg ctttacaggc ggttcgcgag tgaacagcgg gatgatgttt atcaccctca
    51661 agccacgcga cgaacgcagc gaaacggcgc agcaaattat cgaccgtctg cgcgtaaaac
    51721 tggcgaaaga accgggggcg aatctgttcc tgatggcggt acaggatatt cgcgttggtg
    51781 ggcgtcagtc gaacgccagc taccagtaca cgttgttatc cgacgacctg gcggcactgc
    51841 gagaatggga gccgaaaatc cgcaaaaaac tggcgacgtt gccggaactg gcggacgtga
    51901 actccgatca gcaggataac ggcgcggaga tgaatctggt ttacgaccgc gacaccatgg
    51961 cacggctggg aatcgacgta caagccgcca acagtctgtt aaataacgcc ttcggtcagc
    52021 ggcaaatctc gaccatttac cagccgatga accagtataa agtggtgatg gaagtggatc
    52081 cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggca
    52141 aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga
    52201 atcatcaggg attatcggcg gcctcgacca tttcgtttaa cctgccgacc ggaaaatcgc
    52261 tctcggacgc cagtgcggcg atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg
    52321 tgcgcggcag ttttgccggc acggcgcagg tgttccagga gacgatgaac tcgcaggtga
    52381 tcctgattat cgccgccatc gccacggtgt atatcgtgct gggtatcctt tacgagagtt
    52441 acgtacatcc gctgacgatt ctctccaccc tgccctcggc gggcgttgga gcgctgttgg
    52501 cgctggagct gttcaatgcc ccgttcagcc taatcgccct gatagggatc atgctattaa
    52561 tcggcatcgt gaagaaaaac gccattatga tggtcgattt tgcgcttgaa gcccaacggc
    52621 acggtaacct gacgccgcag gaagctattt tccaggcctg tctgctgcgt tttcgcccga
    52681 ttatgatgac taccctggcg gcgctgtttg gtgcgctgcc gctggtattg tcgggcggcg
    52741 acggctcgga gctgcggcaa cccctgggga tcaccattgt cggcggactg gtaatgagcc
    52801 agctccttac gctgtatacc acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc
    52861 gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat gacagatctt cccgacagca
    52921 cccgttggca attgtggatt gtggctttcg gcttctttat gcagtcgctg gacaccacca
    52981 tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc
    53041 acatggtcat tgtctcttat gtgctgaccg tggcggtgat gctgcccgcc agcggctggc
    53101 tggcggacaa agtcggcgtg cgcaatattt tctttaccgc catcgtgctg tttactctcg
    53161 gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact gttgctggca cgcgcgttac
    53221 agggcgttgg cggcgcgatg atggtgccgg tcggcagatt gacggtgatg aaaatcgtac
    53281 cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt acccggtcag gtcggtccgc
    53341 tgctcggtcc ggcgctcggc ggtctgctgg tggagtacgc atcgtggcac tggatctttt
    53401 tgatcaacat tccggtgggg attatcggtg cgatcgccac attgctgtta atgccaaact
    53461 acaccatgca gacgcggcgc tttgatctct ccggattttt attgctggcg gttggcatgg
    53521 cggtattaac cctggcgctg gacggcagta aaggtacagg tttatcgccg ctgacgattg
    53581 caggcctggt cgcagttggc gtggtggcac tggtgcttta tctgctgcac gccagaaata
    53641 acaaccgtgc cctgttcagt ctgaaactgt tccgtactcg taccttttcg ctgggcctgg
    53701 cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc
    53761 tgcaaattgg cctcggtttc tcgccgtttc atgccggact gatgatgatc ccgatggtgc
    53821 ttggcagcat gggaatgaag cgaattgtgg tacaggtggt gaatcgcttt ggttatcgtc
    53881 gggtactggt agcgaccacg ctgggtctgt cgctggtcac cctgttgttt atgactaccg
    53941 ccctgctggg ctggtactac gttttgccgt tcgtcctgtt tttacaaggg atggtcaact
    54001 cgacgcgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac aatctggcga
    54061 gcagcggcaa cagcctgctg tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta
    54121 tcgccgggct gttgctggga ctttttggtt cacagcatgt cagcgtcgac agcggcacca
    54181 cacaaaccgt ctttatgtac acctggctta gcatggcgtt gatcatcgcc cttccggcgt
    54241 tcatctttgc cagagtgccg aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa
    54301 ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt
    54361 cgccacctgc attgtcttgc tgatcagtat gcactgggcg gtgcgtatca gttttgagcg
    54421 tggctttatt gattacatca agcatggtaa tgaacagcga ttacaactgt taagtgatgc
    54481 gcttggcgag cagtatgcgc agcatggcaa ctggcgattc ctgcgcaaca atgatcgctt
    54541 tgtctttcag atcctgcgtt catttgaaca cgataattcg gaagataaac ccggcccggg
    54601 tatgccaccg cacggctggc gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct
    54661 ggttggtccg cgagcgccga ttccacctga cggtacacgg cgacccattc tggtcaacgg
    54721 tgcggaagtt ggcgcggtga tcgcctcccc cgttgagcgg ttaacgcgca atactgatat
    54781 caatttcgat aaacaacagc ggcaaaccag ctggttgatt gtcgccctgg caacgttact
    54841 cgcggcactt gccacttttc tgctggcgcg cggtttactg gcaccggtaa aacgacttgt
    54901 cgatggcacg cacaaactgg cggcgggcga tttcactacc cgcgtaacgc ccaccagtga
    54961 agatgaactg ggcaaactgg cgcaagactt caaccagctt gccagcacac tggagaaaaa
    55021 ccagcaaatg cggcgcgatt ttatggccga tatttctcac gaactgcgta cgccattagc
    55081 ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg cgtaaattca cgccggagac
    55141 ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa ctggttgacg atctccatca
    55201 gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc
    55261 actgctggaa gtggcgggcg gcgcatttcg cgaacgattc gccagtcgtg gcctgaaact
    55321 gcaattttcc ctgccagaca gtattaccgt atttggcgat cgcgaccgtt taatgcagtt
    55381 attcaataac ttactggaaa acagcctgcg ctacactgac agcggcggca gcctgcaaat
    55441 ctctgccggg cagcgcgaca aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt
    55501 cagtgacgat cagctacaaa aattgtttga acgtttttat cgcaccgaag gttcccgcaa
    55561 ccgtgccagc ggcggttccg ggctggggct ggcgatttgc ctgaacattg ttgaagcaca
    55621 taatggtcgc attattgctg cccattcgcc ttttggcggg gtaagcatta cagtagagtt
    55681 accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca
    55741 ccgcgtattt tgatcgtgga agatgaaccg aagcttgggc agttgctcat tgattatctg
    55801 cgtgctgcga gctatgcgcc gacgcttatc agccacggcg atcaggtact gccgtatgtg
    55861 cgccagacac caccggatct gatcctgtta gatctgatgc tccctggcac cgatggcctg
    55921 acgctgtgcc gggaaattcg tcgtttttct gacattccga tcgtgatggt gacggcaaaa
    55981 atcgaagaga tcgatcgcct gctggggctg gagattggcg cagatgatta tatctgtaag
    56041 ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg
    56101 cagcgcgagt tgcagcaaca ggatgctgaa agcccgttga ttatcgacga aggtcgtttt
    56161 caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg cggaatttcg tctgctgaaa
    56221 acgctctctc acgaaccagg aaaagtgttc tcccgcgagc aattgctcaa tcatctttat
    56281 gacgactacc gcgtagtaac cgaccgcacc atcgacagcc acattaaaaa cctgcgccgc
    56341 aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttatgg cgtcggttac
    56401 cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc gacattattt tgttagccgg
    56461 agatggcgtt cagaccagat ccggcaacat tatcccacgc atggtcagca aactgacatt
    56521 atctctccgc cctggcaggc atcagaaaat cgctcatact ttaatcggta aacagcacct
    56581 ttagatgctg ttttcgatac acaatttcaa tcaaggagtc attatggctg gttggtttga
    56641 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
    56701 tatcctcacc agcgagcttt atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt
    56761 gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa acggcaagta acggcaaatt
    56821 ctatttcaat ctgaaagccg ctaatcatca aattatcggc tccagccaga tgtacgccac
    56881 cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa gccagacggt
    56941 gaaagacaat acgtaattac gatgccgggc gtgggttgtt ggcgtccggc gctttaccag
    57001 atccccccac agcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca
    57061 tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc
    57121 ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct
    57181 gtttatgcgg gccagccgcg ttactccctg cgtgtgcgca acaacgaatt caaccacgaa
    57241 aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc
    57301 aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg
    57361 gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag
    57421 cacttccctg aaatgccgat ccacctctcg gtgcaggcta acgccgtaaa ctgggcgacg
    57481 gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg
    57541 gaagagattg aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac
    57601 ggcgcactgt gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc
    57661 gatccgaacc agggtacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg
    57721 aaagaagatg acgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt
    57781 gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt ttatgatcga agaagcccag
    57841 cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg
    57901 aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg
    57961 aaaatcgaag gccgtactaa atctttctac tattgcgcac gcaccgcaca ggtttaccgt
    58021 aaagctatcg atgacgccgc tgcgggcaaa ccgttcgata ccagcctgct ggaaactctg
    58081 gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat
    58141 tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt
    58201 accggtgagc gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc
    58261 gacagccttg agctgatgac gccgcaaggc aacattaact ttacccttga gcacatggaa
    58321 aacgccaaag gtgaagcgat gccggtcgca ccaggcgatg gttatactgt gtggctcccg
    58381 gtcccgcagg atcttgagct caattacgct ctgctgatgc gtaatttctc cggggaaacc
    58441 acgcgtaacc cacacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt
    58501 agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat
    58561 aacccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac
    58621 acgggcttat ttttttatgc ataagcccta tctctggtaa ccgtcttcca ttgaccacat
    58681 cgatagaatc ctccttcata gcacgatgcc tttcacttat cggcatcgtg ctcccacagg
    58741 ttccggctac gcacagccag aacgcgcata tttgacgctt accaaaaaat attctcactc
    58801 tccacatttg aatgtcagac gagcgacacc atgtaatcct acaccttctg tcttcagctc
    58861 aactatttgc atttttttgc cctgagtaac acagaaatga gttgcatcat tttttactat
    58921 attttctgca ccagatattc tacccctggc taaagaagct tcggcttcgg tgtagtattg
    58981 gttatcgagt ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt
    59041 ctcatcatta tgcacagcac aaccagacat aataaaaata ctaattaatg atatagcagc
    59101 tatctttttc atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata
    59161 taataatggc atgattatta taattgaata ggattataat aaatgttctg tacaacattt
    59221 cctacataag taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta
    59281 atatattata ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa
    59341 atttatcggt ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca
    59401 ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc
    59461 accttttgtt ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg
    59521 gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg
    59581 cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg
    59641 gagaaaggcg atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg
    59701 attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag
    59761 ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc
    59821 agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtgacgcc
    59881 attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc
    59941 ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtagc
    60001 gtctcttaca tcattcatgg cttaatgcgt atggatactc tgcaaccgga ccgttgtgaa
    60061 atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg
    60121 cgtcaggccg gtggcggtca gcaattgtgt ccgaacgcgt taattaacga tggcttgctg
    60181 caactgcgca tttttaccgg cgatgaaata cttccggctc tcgtatcaac cttaaaatct
    60241 gacgaagata acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat tcaggcacca
    60301 cacgacatca cctttaatct tgatggcgaa ccgttgagtg ggcaaaattt tcatattgaa
    60361 atacttccgg cagcgttgcg ttgtcgatta ccaccagatt gtccactgtt gcgttaatca
    60421 aactattctc tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat
    60481 ccggcataca gaacaatact acaccgtaat taattttacc cgcgtcttct gcaatgccag
    60541 tttatccacc tctgacacag catcatcggt aataataatg tcaatttttt cgattggtag
    60601 tacctgatta aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt
    60661 agcggctgtg accatcgcac cgctaataga ataaccttca ttaaaggtcg taataccatt
    60721 aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat cctgtaatga
    60781 acgctcggca atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac
    60841 gaccagagta atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat
    60901 caccgtgata tttttggcgg tcattaatcc ttcggcgata agcattgtcg tacttccgct
    60961 gtcgagaata acggtcatcc cttcgtggat cattttgact gccgcctgcg ctattctgtt
    61021 ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc
    61081 actattacca gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg
    61141 ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag
    61201 atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc
    61261 gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc
    61321 attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca
    61381 gggaatgagc aacactttgc ccggcatagc attacgagcg atgtcacgca ccgcctgggc
    61441 gaagctttca aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt
    61501 cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggagt agttcatcca
    61561 actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg
    61621 cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac
    61681 cgccagttcg acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag
    61741 ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat
    61801 cgcaccgaaa gattttgcca gtgccagttt ttctgaacta atgtcgatcg ccgtcacact
    61861 ctttgctccc agcgcgacag cgcactgaat ggccagcagg ccaatggttc cggcaccaat
    61921 aataataacg tttttattct cacaaccttg cgctaaatga aaagcatgca gaccaacggt
    61981 aatcggctca ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt
    62041 ttttcgctta acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc
    62101 atattttgcg cactgggaat aaaacccttt cagacactct ggacaagtaa aacagggtaa
    62161 taacggcaca caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc
    62221 atcaatatag ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt
    62281 aaatatcctg ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc
    62341 atcctgatgt ttaatttcag gaatgacgct ttctgcaacg cgcacgatac catcagtatc
    62401 attcaccact gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt
    62461 aaaaattatt ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag
    62521 gtcatgaaaa taccggtcag ataaattgca ccgataataa tgaaaccggg aatattttgc
    62581 ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc
    62641 accatacccc cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata
    62701 gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg
    62761 aacagatttc cacgatgcac ggcgaccgcc atcgccacga agaagccgat ggtggcaaga
    62821 tcgccaaacg gcagcacctg attacccggc acacaaacag caattaaaat ggtgagtggg
    62881 ataaaaatca ggcttgccga taccaccgcc gtatgtccca gcagcaacgc cggatcaagg
    62941 ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgagc ctgcttagcg
    63001 atgggcgtta aaccatccat gatgggttta atcacccgtg gcattagcag catcactgcc
    63061 gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata
    63121 atcagcccca tcacaaaacc cacggtgaca ggctcgccaa atggaccaaa tttgcgctga
    63181 atatcgtcgg cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg
    63241 accagcaccg caatcggccc catatacgcc gacgtaccgt gcggaatagc aataccttcc
    63301 agctcaaaga aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc
    63361 gcgtgaatta ccacacctgc catccctatc atccatgaac cggttgccag atgcagcaac
    63421 gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata
    63481 cgggtcagta acatcgccac gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt
    63541 tgcgacgccc aggtcattgg tgaagagccc ggccagccaa catcgaccac atgcagattc
    63601 aggtcgaaat tttccgccat cgctttcgcc gccgggccaa tggaatccag cattaagcca
    63661 atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg
    63721 cctgccttca tgcctaatat tttagaaaaa ataatgatga caatcggcag catcaccgta
    63781 gggccgaggt cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata
    63841 gtcagaattt tattttgtaa tgcttcgata ccgataccag aaataaaagg catgccgtga
    63901 actaacggaa tatcgccaaa actacgatcc actttggcag tggtgcatat caaatgcaca
    63961 ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta
    64021 tgattctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct
    64081 ccgcaagcga caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt
    64141 tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga
    64201 gtgattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt
    64261 ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtcg
    64321 ttatcatcat ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct
    64381 gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tagcgtgctg ctcaagcatt
    64441 atcccggtag ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc
    64501 acacctttgg cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa
    64561 gaaattccgc tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag
    64621 cgataggcgc gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat
    64681 aattccccgg actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt
    64741 gggatgtcca cgccttcaag attcaccatc atcgtttcga cgctattttt aatccgacta
    64801 tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg
    64861 ttaaaacccg tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca
    64921 attaccgcca ggcaaccgct gcgattttca ggggcgataa gttcctgctc aatttgtgcc
    64981 agtgcaaata tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca
    65041 aagtgatcgc ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct
    65101 tcataaacca ttcgggtgtt ttctatccat tgcgccagcg gctgcgcttc ctgcggctga
    65161 taatggataa tattgctgtg atcaaattcc acacccggct gcaccacgat ggcaatcaca
    65221 cgtgttaacg cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta
    65281 ttggcggcat cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc
    65341 ggaaccggaa cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc
    65401 acactttccg cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac
    65461 ggtatgggat cccccgcgca ggacattgac gcatcaagat gaattttact gaagccggca
    65521 cgaacatatt cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc
    65581 tgccagcagt ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca
    65641 actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa
    65701 ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg
    65761 cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg
    65821 ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa
    65881 atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac
    65941 atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg
    66001 ctcggtcagg taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt
    66061 gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc
    66121 gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc tagaaaaatc
    66181 aagcgccggt gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc
    66241 aattccggtt gcctcggcaa attcacgcgc ctgagcgggg ttggtgtaca acgcatcggc
    66301 ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg cctcgacgct
    66361 gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa
    66421 aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat
    66481 atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc
    66541 catcgcgctg accagcgcca acagattttc tgtaccagca tgagtaaatg tgccgggcgt
    66601 tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tctaccacca cttgcatcgt
    66661 ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag
    66721 catctgcttt gtcgatacga cgtacatttt catatcctgt cgtttgtttt cgatttcaaa
    66781 atataatgaa attatttgtt tttaaatatc gagataacga tcacaaaaac gacaatatga
    66841 aaattgttcg aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc cgtagcaaag
    66901 tgcgcgtgtt tttatgccgg atgcggtgta aacatacgtg gcagtgctat gtaggcatga
    66961 taagacgctt cagcgtcgca tcaggcatag gttgccggat gcagcgtaaa cgccttatcc
    67021 gtcctacgag gtggtgttgt tcgtataagg cgtatcaggc gatagtaatt ttgctatcga
    67081 gataaacgtc ctgcacggcg ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg
    67141 ctttacgtcc aagaatcagc cccattccgc cagcgcgttt gttgataacc gcagtacgca
    67201 ctgcatcact gaggtcagtt tcaccgcccg cagcaccgcc ggagtttatc aacccagccc
    67261 ggcccatata gcagttagct aactgataac gcaccagatc aatcgggttt tcgctggtta
    67321 acttgctgta cacgcgatcg tcggtataac cgtaattaat cgctttataa ccgccgttat
    67381 tttccgccat tttctgtttg acgatgtccg cgccaatggt cgccgccaga tggttcgcct
    67441 gaccagtcag gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac
    67501 gcaaataggc ccacagcact gtcaccatgc ccagctcgtg cgcacgttca aaagccgcag
    67561 aaatttcttc aatctggcga cgtgactctt ccgagccaaa atagatagtc gcaccaaccg
    67621 caaccgcgcc catgttgaac gcctgctcca cgctggcata cagcgtttga tcgtaggtat
    67681 tcgggtaact tagcgtctcg ttgtgattaa gtttgacgag gaatggaatg cgatgcgcat
    67741 aacgccgcga taccgacgcc agcacgccgt aagttgacgc cacacagtta cagcccgctt
    67801 cgatcgccag ttcaacaatg tttttcgggt caaagtagag cgggttagca gcaaatgaag
    67861 ctccggcaga gtgctcaacg ccctggtcaa ccggcagaat agaaagatat cctgtgccag
    67921 ccagacgccc ggtgttgtac aacgtctgca tattacgtaa caccgcaggc gggcgattat
    67981 tgtcaatcat cacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa
    68041 tagtcataca acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat
    68101 ctgtcattac tatgctcccg taaattccga ttggatatcg gctatggatt ttcctggccc
    68161 gccttttgcg ggcaatcata atcctggtcg ttacgggcga gtttttccat cttttggatg
    68221 ttttttcagc gttttctgtt ggctcgattc atcagaaaaa atgttagcgc ggtcaagttt
    68281 tcaccgcaaa ggtatttaaa aggtattatt aagtggtatt gtcatcgcgt accttacatt
    68341 acctgtcatg aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg
    68401 ttgaatggtt tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg
    68461 gttttagtgc cggagaaatt ggctggtcgt atgcctgtac cgccattgcg gcgatcctgt
    68521 cgccaattct ggttggctcc atcactgacc gctttttctc ggcgcagaaa gtgctggcgg
    68581 tattgatgtt cgcaggcgcg ctgctgatgt atttcgctgc gcaacagacc acttttgccg
    68641 ggttcttccc gttactgctg gcctactcgc taacctatat gccgaccatt gcgctgacta
    68701 acagcatcgc ttttgccaac gtgccggatg ttgagcgtga tttcccgcgc attcgtgtga
    68761 tgggcactat cggctggatt gcctccggtc tggcatgtgg tttcttgccg caaatactgg
    68821 ggtatgccga tatctcaccg actaacatcc cgctgctgat taccgccgga agttctgctc
    68881 tgctcggtgt gtttgcgttt ttcctgcccg acacgccacc aaaaagcacc ggcaaaatgg
    68941 atattaaagt catgctcggc ctggatgcgc tgatcctgct gcgcgataaa aacttcctcg
    69001 tctttttctt ctgttcattc ctgtttgcga tgccactagc gttctattac atctttgcca
    69061 acggttatct gaccgaagtt ggcatgaaaa acgccaccgg ctggatgacg ctcggccagt
    69121 tctctgaaat cttctttatg ctggcattgc cgtttttcac taaacgcttt ggtatcaaaa
    69181 aggtattatt gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta
    69241 gtgcggatga atatttcacc tacgcgttac tgttcctcgg tattttgctt cacggcgtaa
    69301 gttacgattt ttactacgtt accgcttaca tctatgtcga taaaaaagcc cccgtgcata
    69361 tgcgtaccgc tgcgcagggg ctgatcacgc tctgctgcca gggcttcggc agtttgctcg
    69421 gctatcgtct tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa ccggtaaacg
    69481 gactgacttt caactggtcc gggatgtgga ctttcggcgc ggtgatgatt gccattatcg
    69541 ccgtgctgtt catgattttt ttccgcgaat ccgacaacga aattacggct atcaaggtcg
    69601 atgatcgcga tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg
    69661 tgctctttat gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccacg
    69721 cagccgcgtt aaagcacact ttggctggat tgaccgtttt cttcctggac caaaggagaa
    69781 taacgcggcc tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg
    69841 tctggcggat gcgttactgg aacgtgaagg caagatcgat ccggatctga ttgggcgtaa
    69901 tattctcgac tgggcgctgc gtttcgacgc ctttaacaaa aacgtactag gtccgacctc
    69961 gaagattgcg cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaacaacgg
    70021 cgtgaccaac ggcgcagcga tgcgcgtctc gccattaggt tgtttgcttc cggcgcgtga
    70081 tgttgattcc tttattgatg atgtagcgct ggcgtccagc ccgacccata aatccgatct
    70141 ggcggttgca ggcgcggtag tcatcgcatg ggcgatttct agtgccattg acggagaaag
    70201 ctggtcagcg attgttgatt ccctgccttc aattgcgcga catgcacagc aaaaacgcat
    70261 cactacgttc agcgcctcac tggcggcgcg gctggagatt gcgctgaaaa ttgtgcgcaa
    70321 tgccgacggc accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag
    70381 cactattgag tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa
    70441 tcgctgcgcc gtcctgtgcg ctaaccttgg cggcgacaca gacaccatcg gtgctatggc
    70501 gacggcaatt tgcggcgcgt tgcatggcgt taacgctatc gatcctgcgt taaaggcgga
    70561 actggatgcg gtaaatcagc ttgatttcaa ccgctatgcc acagcgctgg cgaaatatcg
    70621 tcaacaacgg gaggcggtat gagcggcgct cgattacaca cgctgctgcc tgaattaacc
    70681 acgcgtcagt cggtgatggt cgtcggcgcg gcggtcattg acgtgatcgc cgacgcttat
    70741 gccctcccct ggcgtgggtg cgatatcgaa ctgaaacagc agagcgttaa cgttggcggc
    70801 tgcgcactga atattgccgt ggcgttaaag cgcctcggca tcgaagcggg taatgccttg
    70861 ccgctcggtc agggcgtgtg ggcggagatt attcgcaacc ggatggcaaa agagggctta
    70921 atcagcctga tcgataacgc cgaaggtgat aacggctggt gtctggcgct ggttgagccg
    70981 gatggcgaac gcacttttat gtcattcagc ggtgttgaaa atcagtggaa tcgccagtgg
    71041 ctggcgcgat taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc
    71101 tcgccctgcg gcgaattgtt agtggaatgg ctggaagagc tgcaagacgt gacgccgttt
    71161 atcgattttg gcccacgtat tggcgatatc ccggatgcat tactggcgcg gatcatggcc
    71221 tgtcgacctt tagtgtcgct caatcgtcaa gaggctgaga ttgccgccga acgttttgct
    71281 ttatccgcag agataacaac acttggcaag caatggcagg agaaatttgc cgcgccgttg
    71341 atcgttcgtc tcgataaaga aggcgcatgg tatttcagca acgacgcttc tggctgcatt
    71401 ccggcatttc caacgcaagt tgtcgacacc attggggcgg gcgacagtca tgccggtggc
    71461 gtacttgccg ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca
    71521 gtggcgtcgt gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta
    71581 ctcctcgcac acaaaaacgt atagatcgct gcgacagtgg ctaatgctgt actcaatagg
    71641 ccgttgctgt tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa
    71701 ctgaatatgt gactgaaact cggcatccgg catccgggcg ctaacgcggg aacgcgtacg
    71761 ttgtgggtaa atgtgctggc tgcggaaata gtcataaagt gaaatcccga tggcatcaac
    71821 atcatgaatt aaatgcgccg gaacccacga ttcctcaatc gataccgctt cttcatcgac
    71881 atagcgaata cgcttaagca aaaagacatc acttcctgct tcgaccgcca gctgctgcgc
    71941 gacttcttcg ggacatttca cgacacgctt gttaacccat agcgtatcgg gctttttccc
    72001 gcgcaatacc acctgctgag aaaaacctcg cgcttctttc aacgagtatt cgaagatgtt
    72061 gttgatctgc gtgccgtaac cgcgcgaacg cgtcaccaca ccctcttctt ccagcgcctg
    72121 catcgccttg cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc
    72181 gggcaaaata ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc
    72241 aaacttaata taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat
    72301 cagctgggta tgcgcttgtt ccatttgtct ttttcctgcc ggggagtctg gtgggagtat
    72361 atcactgctt cttatttaag gcgtttcttt aatcccatca acgaatgcct gcaactcctc
    72421 caccgtgcca ttaaatacat taaaatccac cggaccattg atgccatcta cctgtccacg
    72481 gtcggaatgc tgccagaagc gccaggccat tccgtcattg tccggacgat gttgatagta
    72541 gtgagccacc caccacggat attcattgaa atagcccgcc agattggtgt gataaaaaac
    72601 ggctcctgag taaataatcg gctttttccc cgtacttttt tcgaccattt ttagccactg
    72661 acttacccgc ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagcac
    72721 ggcagggaaa tcgccttgtg agaaatccac cgtttgcaga aataatctcg cctgaactga
    72781 agcggatacc gacggggaga aataatgata cgccccacgc agcaggccat tttcgcggct
    72841 tagttgccag ttacgcgaaa aataggggtc caccagcttt tcgccctccg tcgccttaat
    72901 aaaagcaaac tgtaagcgga taccgttgtc gcgcattttt gccacccgct gccagtcgat
    72961 ccgctcctgc cagcgcgaaa catctattcc gtgaatggtg taactggcag gaatgcgaat
    73021 agcgaaagat ttaaccgggc gataaccgta gaagtggatg tagtctgtaa tctgaactgc
    73081 tgtcgaatag aaaaaattca ccgtctgacg cggataaatt gcaacgatgg aaattagccc
    73141 aagagcgcat aatagagagg tgagtttttt tcgactggta attcttagct gcattggttt
    73201 catccctgaa tgtcagtgcc aaaggctgac aataaccaaa gcagctatag tacggtgctc
    73261 accaccaggc gtggaagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg
    73321 cctgggctaa cgccgatgaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg
    73381 tatggcgcgg gcgtagtgca gccaacgccg cagagagtgt acaaccagtg ccgtgggtgt
    73441 ttttggtcat aatgcgcggt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt
    73501 ccgggctttg ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg
    73561 ccaacagcga tcgcccttgt tccagcattt cctgttcggt gcgcgcgtgt ggcgcgtcga
    73621 gcaaggcggc agcttcgggc aagtttggcg ttattaatga aacctgtggc aataatcgac
    73681 tgcgcagcgt agcaaccgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa
    73741 cggtgtcgag taccacgttt tggatctgat aacgttgcaa ccgttctgcc accgcttcaa
    73801 caatatcggt ttccgccagc ataccgattt tagtggtatc gattcgcaca tcgctgaaca
    73861 ccgaatcgag ctgggcggcg acaaaatcag gctcaatgcg atacaccgac tgtacgccac
    73921 gggtattttg cgccaccagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga
    73981 aggttttaag atcggcctga atccccgcac caccactcgg atcagtaccg gcaatcgtca
    74041 gagcgttaat tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa
    74101 atgtggaaca aaactgcctg gcccctcgct tctggcgact gcgcgttctc cggcttgttt
    74161 catccagtga caggcagatg cgacattttc cagcgtatcg cctggtaacg cacagcaggc
    74221 agcgacaacc gccgataatg cacagccagt tcctaccact ttggtcatta acggatcacc
    74281 accgtgaata ccaatgatac gatgtccatc ggtaacataa tccatctcgc cagtgaccac
    74341 gacgattgcg ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc
    74401 gtcagtggta tccactcccc gtccgccatt agcaatccca gctaatgcca tgatttccga
    74461 agcattacca cgtatcgctg ccggtttaaa agataaaagt tcatgacaaa aatggcggcg
    74521 ataatcgagc gcacccaccg ctactggatc aagcgtccag ggtgtttgag agctttttgc
    74581 ttgctcaacg gcagcacgca tcgcctgagc gcgtggctgc gtcagtgtgc caacgttaat
    74641 caacaaggca ctggcgatag ccgcaaactg actggcctct tcggtttcga taaccatcgc
    74701 tggcgatgca ccgagcgcca gcaaggtatt ggcggtaaag gtttgcacca catcattggt
    74761 catgcagtgc acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc
    74821 tgaacccagc aggtcgattt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc
    74881 tggtggtccg tgacttccct acgctggcat tatccagatc aggtgatacg ggtatttctc
    74941 agccttcacg cagaagggca ccccgagtcg tttggttgcg atgataaatc gcagaggagg
    75001 atggtaatgt ccagcgcacg cgttgtaaac gaggaaaaac ggcagtttta caatcgcgtt
    75061 aataaattat ttgatatatg aatccagcac cttcagaacg acatccagat cttcttcacg
    75121 ttttagctca tccccctggt gaacgatgtg ttccgtcaga tgacctttaa tcacttcccg
    75181 catcagaccg tttaccgcgc cacggatagc agcaatctgt tgtaaaactg cagcgcattc
    75241 gtgcggctcg tcgagcattt tcttgagcgc cacgacctgg ccctgaatct tactggcacg
    75301 cgctttcagt ttctgtttat cacggattgt atgagacatg gcaacacctg gttaacaaga
    75361 atatgaaaaa tcatagcact attaatctac tggggggtag tatcaggtac tgggggggag
    75421 tagaatcaga ttgccgaatt aatactaaga attattatca tgaccgaatt tacaactctt
    75481 cttcagcaag gaaacgcctg gttcttcatc cccagcgcca tcttacttgg tgcgcttcat
    75541 ggcctggaac cagggcactc aaaaacgatg atggcggcgt ttatcatcgc catcaaaggc
    75601 accattaaac aagcggtgat gctcggactg gcagcaacta tttcgcatac cgcagtggtc
    75661 tggttaattg cctttggcgg gatggtgatc agcaagcgct ttactgctca atcagcagaa
    75721 ccgtggctcc agctgatttc cgcagtgatc attattagca ccgcgttctg gatgttctgg
    75781 cgtacctggc gcggcgaacg caactggctg gagaatatgc acgggcatga ttatgagcat
    75841 catcatcacg atcacgaaca tcaccacgac catggacatc atcaccatca cgaacatggc
    75901 gagtatcagg atgcccatgc acgagcccat gccaatgaca ttaaacgacg ctttgatggt
    75961 agagaggtca ccaactggca aattttgtta tttggcttaa ccggtggcct tatcccctgc
    76021 ccggcagcaa ttaccgtgct gttgatatgc attcagttga aagccctgac actgggcgca
    76081 acactggtcg tcagtttcag cattggcctg gcgttaacgc ttgtcaccgt aggcgttggc
    76141 gcagcaatca gcgttcagca ggtcgcaaaa cgctggagcg gatttaacac tctcgctaaa
    76201 cgcgccccct atttttccag tctgttgatt ggcttagtcg gcgtgtatat gggcgtacat
    76261 ggcttcatgg gcataatgcg ataactcaat cctttaggct tgcatgttat cttcagccca
    76321 ggatgattct taaatcagct atttccgctg actctctgct cgctaaggat gcttttaggg
    76381 catcctttca tttacacttt ttacgaaatc atgggatcac taacaaaata tcgcttgtca
    76441 gttatattgt atggcaggaa agatatgcga ctgatattac agatccccaa agtggagagt
    76501 ttatgaccat taaaaataag atgttgctgg gtgcgctttt gctggttacc agtgccgcct
    76561 gggccgcacc agccaccgcg ggttcgacca atacctcggg aatttctaag tatgagttaa
    76621 gtagtttcat tgctgacttt aagcatttca aaccagggga caccgtacca gaaatgtacc
    76681 gtaccgatga gtacaacatt aagcagtggc agttgcgtaa cctgcccgcg cctgatgccg
    76741 ggacgcactg gacctatatg ggtggcgcgt acgtgttgat cagcgacacc gacggtaaaa
    76801 tcattaaagc ctacgacggt gagatttttt atcatcgcta aaaaaagccc cctcatcatg
    76861 agggggaaat gcagacacct tgttattttt tattattagc cacttgctcg tcttgcttgt
    76921 tattagtcgt atttcacgtt gattaatgcg gttgcctcca gtgcgccaga tttaactttg
    76981 tttgtatcgt agacgtagta actggctgtt atcggaattg aaaccgttgt atctgtaagt
    77041 tttgacttcg ccgcaacttc ctttaaattt gtatatcctt tactgctgtt agtgatattt
    77101 aaaagtgtga ctggcgaacc attactttca agaataaatc ctacgccatt atcttccgca
    77161 cctttcagta ttgtgttgct gttggttaac aagtcagaac ttgataaata cacctgcatt
    77221 ttccgtgatg tttgattttg ttgacctaat aaatttttac aacgcagaat aatatcccct
    77281 tgtttactat ttattgttgc tttgttacct gtggctggta attgtgaaac gggaatactg
    77341 tctaaagtaa ttaataaatc gtccggtttg caggttgtgg ttatcgggtt gtatgacaat
    77401 gtcagattaa aggaatagac acctgcattt tgtgccaatg catttctgta gctatttaca
    77461 catgcatccc atgacttgcc acctaataaa cacagtagaa attgtactgc ggcactcccg
    77521 cgtgtcgatg atgaagcact ggataatgtc gcaacaccat taatcatcac tgaaccgccg
    77581 ccccccgcag aaacattcac ttttgcatta ctggccgcag gtgtcaccgt aactgaataa
    77641 ccactggtga aactggtctg attatttgac aacgtgatgt tgtttttatt ggtccattca
    77701 aaatgtaaat ttaattttat ggtatcgtta taaagtccaa caataatatc cgacgggctc
    77761 ataggggtga tagtatttgt acttacgcat gacgaatcat taaaattatg ctcatatgtc
    77821 gccatttgtg tttgagtaag tgcaaaatcc ccctgaacaa ccaagttgtg ctgaacagat
    77881 aactcactat aacatgccgc atagctcaca ggcataacca tcatcattaa tataaatagc
    77941 attatgcgaa tttccataga accacctgac tattactggc aaatataatt tctactttca
    78001 tcaatctctt taccgaaggt gattgtgcat gaaagtcctt gttgcttatc aattgccaca
    78061 ttaaccgatg gcggtacttc attggtgcga ataaataact gacttccctg gccgacaacg
    78121 ccaatattat gaccatggat atcattgact tcataaccaa acgttaatga ttgcccatct
    78181 gctcttaacg cttttataaa ccatggcttg cgctgatcgg tatcaaaatt aaccagtaca
    78241 accgcgccgc gataaggggc ggcaattttc cggttgccac gtaattctgc ttcgctatcg
    78301 ctttgcgaca catccagcat caggtgattt tcccgataag gtgtcattcc gtcgtatatc
    78361 accactccat tacggtttgt tgtgcgatat ttttgcccat tgacataagc atctttaatt
    78421 cctggcgcat tcatcacagc aaacgtttcg gaaagacggt tcgccagatt aacgccaccc
    78481 gaccaggcga caatgccccc tgaaacactg gctccagcct gtcgataagt actcgactga
    78541 ctataactgc cattcactgt cgcaaccggc gcgttccagg tcaaattcgc cccagctgtc
    78601 gtttcatttc cctgatgttg atgactcagg ttgacaccat aattgaactg atcccgactc
    78661 cctactgttc ctgataatcc cgtattattt gaggcaaacc cctgatcatc aaacgtcgtt
    78721 gagttagaca tatatatttg ccgacgaggc gtcgaaacgt catcacccca atcaaaggga
    78781 atcgatataa aaatattaaa acgtttctct tcatgatgat tctcgtcata agcctggctt
    78841 gccgcgaggg tatagcttat ccgtcgcagg ttgttggaat aactcaactg ataatcctta
    78901 ctactgccgc tacgccccca gtaatctcgc cataacgtac ttaatgacac agacccccaa
    78961 ccttctggca atgactggct catattggcg gaaaagctat ttttgcggcc aaaatcgttc
    79021 tggtaataat cggcaatgtc atagacatcg ttttcatcac ggcgataatt atctttattg
    79081 tttgcccaaa cgtgatcgtt aaatgtccgg taatcacgcg acgaataacg ccaggccgcc
    79141 agaccaaaac gcgtcgacgt ttggctcaca aatttgttgt aggcaatttg ataactttgc
    79201 ccgtcaaaca catcgccgtt gtcttgttta ctatgcgact tagtggcatc gacggaaatg
    79261 gcaccaatgc gtgtattcca gccagccccc aaagtaaacg cgtaataatt attcgcgacc
    79321 atcgagccac catacagcgt caataaatta ttaaaaccat actgataacc cgcctggaca
    79381 aaatcacttt gtttgctcgc cccttcaata tggctacgac ccgccgctaa atcatatttc
    79441 gacacgccgg gttgcagcat atttggcacc gctgcataag gcaccagata ggtggttacc
    79501 gagccgtccg cctctttcac gctgacatca agatctgctc caccaccggc caactgcaaa
    79561 tctgtaatcg cgaacgggcc aggaggaacc tctttctgat aaaccacaaa accattctgt
    79621 tcaatagtta ccagcgcgtt actctgagca atcccctgca cccgtggcgt aaaattttgt
    79681 ttcgagttag gcaacatctg catatcacga aacaaccgca cacctctgaa gcgaacagaa
    79741 tcaaaaatat cgcttgatgt gtacatatca cccacgcgaa gcgtgccgag aagttgggca
    79801 aatccacgtt ccagatacag ggtattgctt ttccacaccc ctggattgtt atttgtttta
    79861 ctgaaactgg catcagaatg cagttgccac cccagtaaat ttaacccgct gttaaaacgt
    79921 acatatgtac tcttgttatt acccgacgct ttatagtcgc tgtaatactg actcagataa
    79981 taagaggtat aaaacgcatt aataccccgc tcccagtttt ccggtggaac atagccactt
    80041 tccagttctt ccacccaggc ctgcgggaca ctgaaatcga gacgaaaaac cccgatatcc
    80101 caggtatagc tcccaccctg aacaagttgc tcaaatgtta aacattgctt accgctggcg
    80161 aagttatcgc tattaatgcc taaccgcttg ataacttctc ttgataaaca tgtttcttgc
    80221 gggttgtctt taacaataat ctcatatttc ccgcgccatt gcttattgac ataaatatcg
    80281 atgtcatact gccccggtaa gggttgatta tcatcaagac gaatatttgc aacctgctgg
    80341 tctttcattc cacctatcat aaaatgggta tcaaaggttt cttcagctgc ataagcttca
    80401 atgccgagca ataacgctac gattgctgat gcaagtgggg tcattctcaa cataggcagc
    80461 tcctgcaatt aaattttgtc actaatatag ttgccatggt catcgataat ggtcagatgc
    80521 cagttatttg cattattact tttgacatta acactctgac tttctaaggg ggcaatcata
    80581 atagtttcat aattgacttt gacattatta gctttgacat ccgaaatcgt cacccaatta
    80641 gctgagtcat tttttatcac caaaccattg ccactgcgat ttaccagcaa ttttttaaat
    80701 gtcgctttat ttaccggagc aatacccgcc ggccggtaaa acaacttaat tctgttttgc
    80761 atcgcaaact tcagtgcatt cttgccttct tgctctggac tatttggtgg aatatccaga
    80821 acattcagat aaaaaatgct ttctttatta gtgggcagtt tattcggcat aattttgatt
    80881 tttacttgct gcccggaatt tgcccctatt tttgccactg gtggcgttaa catgaaagga
    80941 acctgaattt tttctggtgg taatgacgta tcgccatcat caatccacgc ctgcagcagc
    81001 gaagaacggt ttccctggtt catcaactgc accatcactt ctttattttc tgccgggtaa
    81061 ataatgcgcg tcccgtagat aacaattccg gcatgtgcag gaaggaccat agaaaaaatg
    81121 agtaaagata ataatccttt cattttaatg cctcttaaat taaaaggccg ccatgggata
    81181 gcggccatgt ttattacctg gagaaactta caattttatt tattaatcct gcgccacacg
    81241 cataaccaga gaagataaca catcacctga ttgcactgag ttatctgtca gtttcaggta
    81301 ttttgcttta aacggaatag catattgacc ttccgctgtt ttataactct caatgttaaa
    81361 aggtgcagaa acatccagga catcgctgtt atcacctaaa tagagatagt aacccacggt
    81421 atcgttgcta ctggctatcg ccttatcgtt accggttatt ttatcgttgg taatcaacga
    81481 gagttttaca ccagcaactt ctttcgggca gctaattttt aacatgctgc tggttacacc
    81541 gactgcatca tcataggtag aatatttagc tttaccttca aagacagtgg ttggtacagt
    81601 cggcatatta atacttactg aatcaccacc cgcttcgaat ttacacggag attctgcaat
    81661 attcccctta atagttaatg ttccggtatc aacgtctgca gcaaatactc cagcgctcat
    81721 aaaaaaagaa gaaaagacag cggcagcaat aattgaacgt ttcataatat gttccttaat
    81781 aaaatttatt tttggttgtt aagagtctgt atctcttgga aacaaatttc tcacatcaaa
    81841 aaagttgccg caatgtaaac acattgtttt attgatttaa aacaaattaa ccattgcaat
    81901 ataaatacaa caaatattta agagtattaa ctatttatcg catctatcaa ttaatgtaga
    81961 tttatgtaaa tggtattgtt gatatcaata aaaaagccga aatcatttat ataaatgatt
    82021 tcggctttct aaatgcaatt aaaatcaggt tgccaaatta atcgtagcga tttaacacga
    82081 ttcgacgttt gctatcgcta cctgcaacaa ccacttctgt cgttacacct ttgacgggcg
    82141 aagaaaacag atgacgggtg tcaacattat acgttaactg accacaatgc tgttgaggtt
    82201 gaccattaac catagccata ctgcatgttg ggggactgat cctgccatta atcgctaatg
    82261 tggcagtctc tgaagagaac gcgggagaag ccaatccata ggcaagaaaa ataattcctg
    82321 acagccaata cttattcatt gaacgttatc cctgtagtaa aggttatgcc tggatagaat
    82381 gagtgcataa caaactatag ctgtacatcc actacacagc cacgaaggat gataatgaag
    82441 cattgcctgt acgatcaatc gactttgtag agtttcggac gaaggtccgc agaatattcg
    82501 cagtattaaa taagtgttca ttaatgcggc aaaaacaaat acaccgcatc aatataacat
    82561 ctctgcggtg tatgaagaat aaccagaatg gttaataggc gttagaccgc gcggaaggaa
    82621 atctcgcctg gaatgacttc accctgccag tagagctgag ctgcaacgcg gtcagccagc
    82681 tggcggtaga tagcggtaaa ttcgctctcc ggacggctga taaccgttgg cgtgccttta
    82741 tcgagatcct cacgtaagga gatgtgtaac ggcatctgcc ccagcagctg tgtgtggtat
    82801 ttttcagcca gtttctcagc accaccggta ccgaaaatcg gttcatggtg accacagtta
    82861 ctgcaaatat gcacactcat gttctcgaca ataccaagca ccggaacctc gactttctcg
    82921 aacatcacaa tgcctttttt cgcatcgatc aacgcgatgt cctgcggggt ggtgaccacg
    82981 actgcaccag ttactggaat gttttgcgcc agcgtcaatt gaatgtcacc ggtgcctggc
    83041 ggcatatcaa gcaccagata gtcgagatcc ggccacaagg tttcctgcaa catctgcatt
    83101 aacgccttgc tcgccatcgg tccacgccac accatcgcat tgtcgtcagt gaccagataa
    83161 ccaattgaat tggttgccag gccatgagac ataatcggtg ccatatgggt accgtccggc
    83221 gaagttggac gctgattttc tgcgcccagc atggtgggaa ttgatgggcc gtagatatcg
    83281 gcgtccagaa tgccgacttt cgccccttcc gccgccagtg ccagcgccag gtttaccgcc
    83341 gtggaggatt tacccacgcc gcctttaccg gaactgaccg caatgatgtt cttcacacca
    83401 ttaatacccg gctgattctt cacgcgtttt aaggtggcga tattgtgcga cagtttccag
    83461 tcgatagctt tcgccccggt gatacgcagc agttcggcgc tacattgctc ttttaactct
    83521 tcaaacgcgc tatgccagac gaatggcata accagttcca catgcaacgt gtcgtccatc
    83581 caggcgacat ggtgcaacgc tttcagcgtg gtgaggttat gcttcagggt tgggtgctga
    83641 aaattggcca gtgtcccggc gaccattgcg cgcaaggctt ctggtgattt ggcctgggat
    83701 tgttcgttca tcccgactcc ttttttgtat aaataaacca tcagctgata gtttacctga
    83761 agaatataga gaagtactta cttaacattt tcccatttgg tactatctaa ccccttttca
    83821 ctattaagaa gtaatgccta ctatgactca agtcgcgaag aaaattctgg tgacgtgcgc
    83881 actgccgtac gctaacggct caatccacct cggccatatg ctggagcaca tccaggctga
    83941 tgtctgggtc cgttaccagc gaatgcgcgg ccacgaggtc aacttcatct gcgccgacga
    84001 tgcccacggt acaccgatca tgctgaaagc tcagcagctt ggtatcaccc cggagcagat
    84061 gattggcgaa atgagtcagg agcatcagac tgatttcgca ggctttaaca tcagctatga
    84121 caactatcac tcgacgcaca gcgaagagaa ccgccagttg tcagaactta tctactctcg
    84181 cctgaaagaa aacggtttta ttaaaaaccg caccatctct cagctgtacg atccggaaaa
    84241 aggcatgttc ctgccggacc gttttgtgaa aggcacctgc ccgaaatgta aatccccgga
    84301 tcaatacggc gataactgcg aagtctgcgg cgcgacctac agcccgactg aactgatcga
    84361 gccgaaatcg gtggtttctg gcgctacgcc ggtaatgcgt gattctgaac acttcttctt
    84421 tgatctgccc tctttcagcg aaatgttgca ggcatggacc cgcagcggtg cgttgcagga
    84481 gcaggtggca aataaaatgc aggagtggtt tgaatctggc ctgcaacagt gggatatctc
    84541 ccgcgacgcc ccttacttcg gttttgaaat tccgaacgcg ccgggcaaat atttctacgt
    84601 ctggctggac gcaccgattg gctacatggg ttctttcaag aatctgtgcg acaagcgcgg
    84661 cgacagcgta agcttcgatg aatactggaa gaaagactcc accgccgagc tgtaccactt
    84721 catcggtaaa gatattgttt acttccacag cctgttctgg cctgccatgc tggaaggcag
    84781 caacttccgc aagccgtcca acctgtttgt tcatggctat gtgacggtga acggcgcaaa
    84841 gatgtccaag tctcgcggca cctttattaa agccagcacc tggctgaatc attttgacgc
    84901 agacagcctg cgttactact acactgcgaa actctcttcg cgcattgatg atatcgatct
    84961 caacctggaa gatttcgttc agcgtgtgaa tgccgatatc gttaacaaag tggttaacct
    85021 ggcctcccgt aatgcgggct ttatcaacaa gcgttttgac ggcgtgctgg caagcgaact
    85081 ggctgacccg cagttgtaca aaaccttcac tgatgccgct gaagtgattg gtgaagcgtg
    85141 ggaaagccgt gaatttggta aagccgtgcg cgaaatcatg gcgctggctg atctggctaa
    85201 ccgctatgtc gatgaacagg ctccgtgggt ggtggcgaaa caggaaggcc gcgatgccga
    85261 cctgcaggca atttgctcaa tgggcatcaa cctgttccgc gtgctgatga cttacctgaa
    85321 gccggtactg ccgaaactga ccgagcgtgc agaagcattc ctcaatacgg aactgacctg
    85381 ggatggtatc cagcaaccgc tgctgggcca caaagtgaat ccgttcaagg cgctgtataa
    85441 ccgcatcgat atgaggcagg ttgaagcact ggtggaagcc tctaaagaag aagtaaaagc
    85501 cgctgccgcg ccggtaactg gcccgctggc agatgatccg attcaggaaa ccatcacctt
    85561 tgacgacttc gctaaagttg acctgcgcgt ggcgctgatt gaaaacgcag agtttgttga
    85621 aggttctgac aaactgctgc gcctgacgct ggatctcggc ggtgaaaaac gcaatgtctt
    85681 ctccggtatt cgttctgctt acccggatcc gcaggcactg attggtcgtc acaccattat
    85741 ggtggctaac ctggcaccac gtaaaatgcg cttcggtatc tctgaaggca tggtgatggc
    85801 tgccggtcct ggcgggaaag atattttcct gctaagcccg gatgccggtg ctaaaccggg
    85861 tcatcaggtg aaataatccc ccttcaaggc gctgcatcga cagcgccttt tctttataaa
    85921 ttcctaaagt tgttttcttg cgattttgtc tctctctaac ccgcataaat actggtagca
    85981 tctgcattca actggataaa attacaggga tgcagaatga gacactttat ctatcaggac
    86041 gaaaaatcac ataaattcag ggcagttgag caacagggaa acgagttgca tatcagttgg
    86101 ggaaaagttg gcaccaaagg gcaaagccag ataaaaagtt tttcagatgc tgcggcagcg
    86161 gcaaaagcgg agcttaagct gattgcggag aaggtgaaga aggggtatgt ggagcaagcg
    86221 aaggataatt ctttgcaacc ttcccaaacg gtaacgggct ctctcaaggt agcggactta
    86281 tccaccatta ttcaggaaca accctctttc gtagcagaaa cccgtgcgcc tgacaaaaat
    86341 acagatgctg ttttaccgtg gctggcgaaa gatattgctg tcgtttttcc gcccgaagtt
    86401 gtacacacca cgttaagtca tcgccgcttt cccggagttc ctgttcagca agcagacaaa
    86461 ttgccccaat tacgtcgctt agcctgtagt gtgtcgcaac gggataataa aacagccaca
    86521 tttgacttca gcgcctgttc tttagaatgg caaaacaccg tcgcccaggc gatcagtcag
    86581 atcgacggcc tgaaaacaac acagttacca tcaccagtaa tggctgtact cacggcactt
    86641 gaaatgaaat gcacaagata taaagtgcgt gaggatgtta tggatcagat cgtccaagaa
    86701 ggcggtctgg aatatgctac tgatgtaata atacaccttc aacagattga tattgaatgg
    86761 gattatgcga ataatgtcat tattattctg ccgtctggca ttgcacctag ctacttggag
    86821 caatattcca gatttgaatt acgcctacgt aaacatttat cactgacgga agagtctctc
    86881 tggcaaaaat gtgcacaaaa acttattgcc gcaattccac atattccaga atggcggcaa
    86941 ccattaattg ctttgttatt acccgaaaaa ccagaaattg cacatgaaat tgcccagcgt
    87001 ctactggggc aaaaaaaatt accctcgctt gagtggttaa aaatagtggc gactgatgag
    87061 cacattcttg cctcattaga aaaatatcac gaaccatatg ccatttttga tgattactat
    87121 tgtggtgcga tatggtcagc caccgtatta caggagcaag gtgttgcagc cctgccccga
    87181 tttgctccct atgccgcaag tgactactgc gccgatgtgt tgcgtcatat caatcatccg
    87241 ttcgcattga cactgcttat acgtgtagcc gggcaaacta aacgctgtca cgatcggatg
    87301 acgaaagcca ttgctgcgtt cccacatgca gcaatggcgg cactgacgga acttcttggg
    87361 caaaaagaag agaacagttg gcgcattatg ctaatgacaa tgcttatctc acaaccagca
    87421 ctggcagaac aggtcattcc ctggctctcg acacccgcag ttgccgtact gaaatcatgc
    87481 caggaacaac tgacacagcc ctcaaaccat gccagcgccg atctactgcc agccgtagta
    87541 gtctcccctc cctggctttc gaaaaagaaa aaatcgccga ttccggtgct ggatttagcg
    87601 ccattaggca ttgagccaat ctgttatctg acagaagaaa tcagtaatca acttttggcg
    87661 aaatatatct ggtatttaaa acacatcacg gttagccatg aagaaagtac taccaacctg
    87721 ttggcaagga tgggttttca acgacggatc gctggtacat atattaaagc tcccgaagcg
    87781 gtagttgagg catggctaaa tgaagattat tcaaccttac taagtgaatt taaggtgttt
    87841 cattcaccta ccgggcatta ttggcagttg gggattttga caacattgcc gctggagaaa
    87901 gcagtaaaag catggaatgc ccttacccta tctccacata ccgataccga atacgccatg
    87961 ttacattttg gactcaaagg gttacctggg ttagtaaact cacttgcacg ctatccacaa
    88021 gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc cgtcgcccgt
    88081 gccttcaaca aactgaaaac gctacgcgaa aacgcccgta gctggctgtt gaaatacccg
    88141 gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga agcacaggat
    88201 aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt actgcaagaa
    88261 atcgcccgac gttataacca gccggaagta accgatgcgg tgaacgctct gcttgcgctc
    88321 gatcccttag ataatcaccc gacaaaaatc cccactcttc cggcctttta tcagccatcg
    88381 ctctggacgc gcccggtatt aaaagcaaat gcccaatcac tgccagatag cgccctcctc
    88441 cacctcggtg aaatgctccg cttccctcag gaagaggctc tgtatccggg attattgcag
    88501 gtgaaagacg tctgttccgc cgactcactg gcgggatttg cctgggatct gtttaccgcc
    88561 tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc
    88621 ggtaacgatg acaccgcccg caaactgacg ccattaatcc gcgcctggcc tggcgaatcc
    88681 cagcataaac gcgccaccgt tgggttggat attctcgctg ctatcggtag tgatatcgcc
    88741 ctgatgcagc ttaacggcat tgcccagaaa ctgaaattca aagcattaca ggagcgggcg
    88801 aaagaaaaaa ttgccgacat tgccgaaagt cgcgaactga cagtggcgga gcttgaagat
    88861 cggttagcac cggatctcag tctggatgat aacggttcgt tgctgctgga ttttggccca
    88921 cggcagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga tgtttccggc
    88981 agccgcctga aagacttacc taaacccaat aaaagcgatg atgaatcgcg ggctgatgag
    89041 gcggtaaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc ccagcaggta
    89101 gcaaggctgg aatccgccat gtgcctgcgc cgccgctggt caccggaaaa ctttcagctg
    89161 ttcctggttg agcatccgtt ggttcgccac ttaacccgcc gtctgatttg gggtgtttat
    89221 agcgccgaaa accagctgct ggcgtgcttt cgcgtggcgg aagataacag ttacagcacc
    89281 gctgacgatg atcttttcac catgccggaa ggcgatatct ctattggtat tcctcacgtt
    89341 ctggaaatat caccgacgga tgctgccgcc tttggtcagc tttttgccga ctacgaactg
    89401 ttaccgccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac
    89461 gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggtcgggt aatggggctg
    89521 gcgaataaag gctggataaa gggcaccccg caggatggag gctggatcgg ctggatgatc
    89581 aaacctctcg gtcgctggtc gttaatcatg gaaatcgatg aaggctttgc tgtaggtatg
    89641 tcgccagccg aactcagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
    89701 gcagaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
    89761 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
    89821 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
    89881 ggcaaaacgc cactcagcca tcgcaattgg ccgggcgaac cggtgtctgt tatcactgaa
    89941 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
    90001 gtctggcgtt atgaaaattt ttcactggag tggcagtccg ccatcacgca ggccatcaac
    90061 ttgatcggcg aacacaaacc ctcagtcccg gcccagacaa tggcggcgct agcctgtatc
    90121 gcgcaaaatg acagccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
    90181 gcgactgagg tggtgattgc acgccagttt attgcgcggt gttatgagag tgatcctctg
    90241 gtagtgacat tgcagtatca ggacgaggat tatggctatg gttatcgctc agaaacctat
    90301 aacgaattcg atctccgact gcgtaagcat ctctctctgg cagaggaaag ctgctggcag
    90361 cgttgcgccg acaaactcat tgccgcacta ccaggaataa ccaaagttcg ccgccctttt
    90421 attgcgctga tcctcccgga aaaaccagaa atagccaatg agttggtagg ccttgaatgc
    90481 ccgcgaactc attttcattc taaggagtgg ttaaaagttg ttgctaatga ccccacagcg
    90541 gtgagaaaac tcgaacacta ctggagccag gatatattta gcgatcgaga agccagctac
    90601 atgtcgcatg aaaaccactt cggctacgcg gcctgcgccg cccttttgcg cgaacaagga
    90661 ctggcagcca ttccgcgcct cgcgatgtat gcccataaag aagattgcgg cagtctgctg
    90721 gtacaaatta accatccgca agtcatccgc accttgctac tggtggctga taaaaacaaa
    90781 cccagcctgc aacgtgtagc taaataccat aaaaacttcc cccatgcgac gctcgccgca
    90841 ctggcagaac tgctggcgtt aacagaacca ccagcccgcc ctggttatcc aatcatcgaa
    90901 gacaaaaagc tgcctgcaca gcaaaaagca cgcgatgaat actggcgtac gctgttacag
    90961 acgctgatgg catcgcagcc acaactggca gaagaggtga tgcagtggtt aagtactcaa
    91021 gccagggcag tgctgaatag ttatttatcg gcaccgccca aaccggttat tgatagtacc
    91081 gataacagca atctgcctga aatcctcgtt tcgccaccgt ggcgtagtaa gaaaaaaatg
    91141 acagctccac gtcttgattt ggcaccgctc gaattaactc cgcaagttta ctggcaacca
    91201 ggcgaacaag agaggcttgc cgccactgag tctgcccgtt atttcagcac ggaatctctt
    91261 gcgcaacgca tggaacaaaa aagtggacga gttgtattac aggaactggg ttttggggat
    91321 gatgtatggc tgtttctgaa ttatatactc cccggaaaac tggatgctgc acgcaattca
    91381 ctcattgttc agtggcatta ctaccagggg cgggttgaag agatcctgaa tggctggaac
    91441 tccccggaag cacaattagc agaacaggcg ctccgcagcg gtcacataga agcgttaatt
    91501 aacatatggg aaaatgacaa ctactcacgt tatcgtccgg aaaagagtgt ctggaacctg
    91561 tatttattgg cacagttgcc gcgtgagatg gctttgacct tctggctgcg tatcaatgag
    91621 aaaaagcatc tgttcgcggg tgaggactat tttctcagta tcctcggatt ggatgcgcta
    91681 ccaggtctgc tgttggcttt ttcacatcgt ccaaaagaaa catttccgtt aattttaaat
    91741 ttcggcgcaa cagaactggc cctgcccgtt gcccgcgtct ggcaccgttt tgcgggccag
    91801 cgtaatctgg ctcgccagtg gattttacaa tggccggaac atacggctac tgcacttatt
    91861 ccactcgtct ttgttaaacc ctgcgacaac agcgaagcgg cattatttgc ccttcgttta
    91921 ctgtatgaac aaggacatag tgaattactg caaacggttg caaaccgctg ggatcgcgct
    91981 gatatgtggc cagccctgga aaaaatactt acccagaacc cgatggaaat ttacccggca
    92041 cgcattccaa aagcccctga tttctggcat ccggcaatgt ggtccaggcc tcgacttatc
    92101 actaataatc agcctgttac cggtgacgct ctggaaatta tcggcgaaat gctgcgcttt
    92161 acccaggggg gacgttttta tagcgggctg gaacaactga aaacgttctg ccagccacaa
    92221 acgctggcag cttttgcctg ggatctcttc actgcgtggc aacaagctgg tgcccccgca
    92281 aaagacaact gggcatttct ggcgttaagt ctctttggtg acgaaagcac ggcacgggat
    92341 ctgacgacac agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc tgtcagcggc
    92401 ctgaacatcc ttaccctgat gaataatgat atggcgctga tacagctgca tcatatatcg
    92461 caacgggcta aatcccgccc cttacgtgat aacgcggcgg aatttcttca ggtggtcgca
    92521 gaaaatcgcg ggctaagcca ggaagagcta gcggacagat tagtcccaac cctgggcctt
    92581 gatgatccgc aggcgttgag ttttgatttt ggtccccggc agtttaccgt tcgcttcgat
    92641 gaaaacctca acccggttat ctttgatcag caaaacgttc gccagaaaag cgttccccgt
    92701 ttgcgcgccg atgacgatca actgaaagcg cacgaggcac tggcccgact aaaagggcta
    92761 aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc gtcttgaagc tgccctacgt
    92821 accacccgac gctggtcgct ggcagatttt cattctctgt ttgttaatca tccctttacc
    92881 cgtctggtta cccagcgatt aatatggggg ggttatccgg caaatgaacc gcgtcgttta
    92941 ctcaacgcct ttcgtgtggc cgcagagggg gagttctgca atgcgcaaga tgagccaatt
    93001 gacctgcctg cggacgctct gattggcatt gcccacccgt tagaaatggc agtagaaatg
    93061 cgcagtgaat ttgcacagct ttttgccgat tacgaaatta tgccgccttt tcgccagttg
    93121 tcgcgccgca cggtgctgct cacacctgac gagtcaacca gtaacagcct gactcgctgg
    93181 gaaggtaaat ccgctaccgt tgggcaactt atgggaatgc gatacaaagg ctgggagtca
    93241 ggctatgagg acacatttgt ctatgacctg ggcgagtacc ggctggtcct taagttttca
    93301 cccggtttta accactacaa tgttgatagc aaagcgttaa tgagctgccg ttctcttcga
    93361 gtgtaccgtg acaataaatc cgtcactttt gccgaacttg atgtgtttga tttgagtgag
    93421 gcgttaagcg cacctgacgt cattttccat taaaacacag cgggcagtga tgcgactgcc
    93481 cgttatctac acgacttacc agcggggaaa gcgatgatcg tgcaaaaaga gctggttgct
    93541 atttacgatt atgaggtccc tgtacctgaa gatccgtttt ccttcagact tgagatccat
    93601 aaatgccctg aattatttac aggttccgtc tatcgactgg agcgattccg gctacatcca
    93661 acatttcatc aacgtgatcg agaagatgct gacccgctaa taaatgatgc gttgatttat
    93721 ataagacatg agtgtaatga tgagcggaaa ttacgaggtg aatcacctga aactgtaata
    93781 gcaattttta atcgtgaact acagaatata ttcaaccaag aaatagaata atatactcta
    93841 aataattcaa attggtccga tccggcgcaa cgtcccaatg gcctggatta taaatctcat
    93901 tatcttaatt gcaacggggt ccagccgtgg ttataattcg ttcgttggaa cacgatgttg
    93961 cacaggctgc ggtgttgggc ctgaaaatag taaataaaag aacctgcctc accagcaggt
    94021 ttttttattt actgtgatct gctttccaga tattttccgc tcaaacaact aatgcgccaa
    94081 acatttattg cgcgtaaaat atcgtttatt tcattaatac atttcaggga tgaatatatg
    94141 tcaccgcaga ataatcatct gcagcgtccg cctgccgctg tgttatacgc cgatgaactg
    94201 gcaaaattaa aacaaaatga taacgcacct tgcccgcccg gttggcagtt aagtttgcct
    94261 gcggcccgtg cttttatcct tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt
    94321 agtccctctg ctgtcgaacg tatgttagtc actcttgcca ccgggcgcgg tttgatgttg
    94381 gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat tactggcaac cgctattagc
    94441 ggcgatgccg ggttaaccat ccagggcggc gcatctacca ctgaagatca aatcaaatac
    94501 ggctggaact acgctctgct tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc
    94561 ccgctttacc agggaatgcg cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca
    94621 ccgctggaag tgcaagactg tctgttaggt atgctttctg accgggttat gacggtgccg
    94681 gaactcacca gtgaagccag tcagctctat gcgcgagaag gctttaatat tattgccact
    94741 gccaataccc gcgatcgcgg cgtcaatgag atgagtgccg cgctaaagcg tcgcttcgat
    94801 tttgaaactg tctttccgat tatggatttc gcccaggagc tggaactggt cgtcagtgct
    94861 tcggcgcgtt tgctggccca tagcggtatt ccgcataaag tgcctgacgc ggtactggag
    94921 ttactggtcc gcaccttccg cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat
    94981 accctgacgg caattatgtc caccgctgaa gccgtgaacg tggcccacgc agtgggcgtc
    95041 cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg acctggtgga ctgcattgcc
    95101 ggaaccatcg tcaaagataa tgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag
    95161 cgtgtcgcca cacataaaga agctcactgg caggcttatt atcaagcccg ccaccgcctg
    95221 ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc atccggcatc atagtccggc
    95281 ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg ccacgatacg tgttgattga
    95341 aggcctggct gattttaatg accgggtaga cgaactgttt ttagcccacc agcttccggt
    95401 agctatttac agttattgcc agtatcagga cggtgcagcc cccgggcgtg gtgcctggac
    95461 gccatttgct gaattttcgc cggagtggca ggcgctacaa gccgctcgtc gtattcaggc
    95521 acaaacttac ttcatcgatt tgccttgctg ggcgcagagt gaagaagagg acgattcgcc
    95581 tgatacgcaa gaggaaagcc agaccttgct gctgcgtgcc acccgcatgg ataacagcga
    95641 caccctgtgg gatcacttgt tcgaagatga aagccagcaa attgcattac cctctgcgct
    95701 ggcgcactat tttgcccaac tgcgcggcga ttcccccggc gatgtactca atcgtcagcg
    95761 cgaagccttt atgacccgct ggattggatg ggcgatgcag caaaatgatg gcgacgtatt
    95821 ggttgtctgc ggtggctggc acgctccggc actggcaaag atgtggcgcg aatgccctca
    95881 ggaaattaac aagccagaat tgccctcgct ggcagatgcc gttacaggtt gttatctcac
    95941 accctacagt gaaaagcgcc ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc
    96001 ggtatggcaa aactggtgct ggcagtgggg cttgcagaag gccggtgaac aactgctaaa
    96061 aactatcctt acccgtttgc gccagcacaa attgcccgct tctaccgcgg atatggctgc
    96121 cgctcatctg catgcgatgg cgctggcaca gttgcgcggt catacactac cgttacgcac
    96181 tgactggctg gatgccttag caggctcgct gattaaagaa gccctgaatg cgccgttgcc
    96241 gtggagctat cgcggcgtta ttcatcccga taccgatccg attctgctaa cgttgataga
    96301 cacattagcg ggtgacggat tcggtaaact tgccccttct acgccacaac cgcctctgcc
    96361 aaaagatgtc acctgcgaac tggaacgtac cgcaatctct cttccggcgg agcttacctt
    96421 aaatcgcttt aaccccaatg gactagcgca aagtcaggtg ctacatcggc tggcaatact
    96481 ggagatccca gggattgtac gccagcaggg aagtacactg acacttgcag gcaacggtga
    96541 agaacactgg aaattaaccc gcccgcttag ccagcatgcg gcattgattg aggccgcctg
    96601 ctttggtgca acactccagg aagccgcacg ccataaatta gaagccgata tgctggacac
    96661 gggtggaatc ggcagtatca ccacatgtct tagccaggcg gcgttagcgg gtctggcgtc
    96721 cttcagtcaa caattactgg agcaactcac attattaatc gcccaggaaa atcaatttgc
    96781 cgaaatgggc caggcgctgg aagtgctata tgccttatgg cggctggatg aaattagcgg
    96841 tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg
    96901 gctgtgtgaa tctaacggca gaccggatga aaaggagttt cacgctcacc tgcatagctg
    96961 gcaagcgctt tgccatatcc tgcgcgatct acatagcggc gttaatttac ccggcgtttc
    97021 tctttctgcg gcggtagcct tactggagcg acgcagtcag gcgattcatg ccccggcgct
    97081 ggatcgcggc gcggctcttg gcgcactaat gcgtctggaa catcccaacg ccagtgccga
    97141 agcggcgctg gcgatgctgg cgcagttatc cccggcacaa tccggtgagg cgctgcacgg
    97201 tttgctggca ttagcccgtc atcaactggc ctgtcaacct gcatttatcg ccggtttcag
    97261 cagtcattta aatcaactaa gtgacgacga ttttattaac gccctgcccg atttacgcgc
    97321 ggcaatggcc tggctaccgc cacgagaacg cgggacgctg gcgcatcagg tgcttgagca
    97381 ttaccagctt gcgcagcttc ctgtttcagc gctgcaaatg ccgttgcatt gtccaccaca
    97441 ggccattgca catcatcaac aactcgaaca gcaggcactg gcatcgctgc aacactgggg
    97501 agttttccat gtctgaactg aacgatcttc tgaccacccg tgagctacaa cgctggcgat
    97561 taattcttgg cgaagcggca gaaacgacgt tttgtgggct ggatgacaac gcccggcaga
    97621 tagaccacgc gctggaatgg ctgtatgggc gcgatcctga acggctccag cgtggtgaac
    97681 gctccggtgg attaggtggc tcaaatctca ccacccctga gtggatcaac agtattcaca
    97741 cgctgtttcc gcaacaggtg attgagcggc tggaaagcga tgccgtactg cgctatggca
    97801 ttgaagatgt ggtgacgaat ctcgacgtgc tggaacgtat gcagccttct gaaagcctgc
    97861 tacgcgccgt tttgcatacc aaacatctga tgaaccccga agtactggct accgcccgcc
    97921 ggatagtgcg ccaggttgtt gaagaaatta tggctcgact ggcaaaggaa gttcgtcagg
    97981 ctttttctgg tgtccgcgat cgccgtcgcc gctcatctat tccactggcg cgaaactttg
    98041 atttcaaaag tactctacgc gccaacctgc aacactggca cccacaacac ggcaagttgt
    98101 atatcgaatc cccccgcttt aacagccgca ttaaacgcca aagcgaacaa tggcaactgg
    98161 tcttactggt tgatcaaagc ggctcgatgg tcgattcggt gatccactct gcggtgatgg
    98221 cggcctgttt atggcagtta cccggcattc gtacccatct ggtggcgttt gacaccagtg
    98281 tggttgatct cacggcagac gttgccgatc cggtagagtt attaatgaaa gtgcaattag
    98341 gcggcggaac caatatcgcc agtgccgtgg aatatggtcg gcaacttatt gaacaaccag
    98401 caaaaagcgt cattatcctc gtgagcgatt tttacgaagg gggttcatca tcattgctga
    98461 cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt gctgggactg gcggcgctcg
    98521 atagcacagc aacgccttgc tatgaccgcg atacggccca ggcgctggtt aatgtcggcg
    98581 cacaaatagc cgccatgaca ccgggcgaac tggcatcatg gcttgcggag aatcttcagt
    98641 catgaattca ctacgtccgg aattattaga actgacaccg caggccctga cagcgttaag
    98701 caatgccggt tttgttaagc gcagtcttaa ggaactggaa aatggcaacg tcccggagat
    98761 cagccatgag aacggcgctt taatcgccac cttcagtgac ggtgtccgta cccagctggc
    98821 gaacggccag gcactgaaag aggctcagtg cagctgcggg gccagcggca tgtgccgtca
    98881 tcgcgtgatg ctggtgttaa gttatcaacg actttgtgcc accgtccagc ccacagaaaa
    98941 agaagaagag tgggatccgg caatctggct ggaagaactg gctaccctgc ccgatgccat
    99001 ccgcaaacgc gcgcaggcgc tggtcgctaa aggcatcacc attgagttgt tctgtacgcc
    99061 gggcgaaatt ccctctgccc gcttaccgat gagcgatgtg cgtttttatt cccgtagcag
    99121 cattcgtttc gcccgttgtg attgtattga aggcacactt tgcgaacatg tcgtactggc
    99181 agtacaggcc tttgtccagg caaaagcgca gcaagcggaa ttgactcatt taatctggca
    99241 gatgcgcagc gaacacgtca catcatctga cgatctgttt gccagcgaag aaggcaaaac
    99301 gtgtcgtcaa tatgttcagc aactaagcca ggcattatgg ctgggcggca tcagccagcc
    99361 gcttattcac tacgaggccg ctttcagccg cgcgcagcaa gcagcggaac gctgcaactg
    99421 gcgatgggtg agtgaatcac tacggcaact gcgcgccagc gttgatgcct tccacgcccg
    99481 cgccagccac tatcatgccg gagaatgctt acgtcagctt gcggcattaa acagtcgatt
    99541 aaattgcgca caagagatgg cgcggcgcga cagtgttggt gaagttcctc ctgtgccgtg
    99601 gcgcacggtc gttggctctg gcattgccgg agaagcaaag cttgatcatc tgcggctggt
    99661 gtctttaggt atgcgttgct ggcaggatat tgagcattat ggtttacgca tctggtttac
    99721 cgatcccgac accggcagta ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga
    99781 aaactcaccg gcagctacgc gtcggctgtt tagttttcag gctggcgcac tggcgggtgg
    99841 gcaaattgtt tcacaagcag caaaacgcag tgccgatggc gagctgctgt tagctacccg
    99901 caaccgctta agcagcgttg tgccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc
    99961 gttacgccag tcgggcattg tggctttgcg ggaatattta cgccagcgtc ccccctcctg
   100021 catacggcct cttaatcagg tcgataactt atttattctg ccggtcgctg agtgtatttc
   100081 gctcggttgg gatagcagcc gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga
   100141 agataatctg ctgacgttat cactgccagc ctcagccagc gcgccttatg ccattgaacg
   100201 catggcggcg cttttgcaac aaacagacga ccccgtgtgt ctggtttctg gctttgtcag
   100261 ttttgttgat gggcaattga cactggaacc acaggtaatg atgacaaaga ctcgtgcctg
   100321 ggcgctggac gcagaaactg cgcctgtggt ggcatcacta ccttctgtca gcgttctgcc
   100381 tgtgccgtct actgctcatc agttgctgat gcgctgccag gcattactta ttcaactgct
   100441 ccataacggc tggcgctatc aggaacagag tgctattggt caggcagagt tgctggcgaa
   100501 tgacctcacc gcggtgggtt tttatcggct ggcacatgta ttgggacaat ttcgtaatac
   100561 agaaagcgag gcacgggtag aagcaatgaa taactgcgtt ttgctgtgcg aacaattatt
   100621 ccccatgctt cagcaacaag gatgaaatag tgctttttac taagagttct actccagttc
   100681 cggactgctc acaccacggt attaggcata tcctatatag cccctggtga gagtcaccag
   100741 ttccttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg ttttcccttg
   100801 ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa gaatcgaaga
   100861 aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac aaaggtgaca
   100921 aggtgcttaa gcaatcttca gaaaccaaaa ttcaatttgc ctctattggt gcaaccacca
   100981 aagaagacgc tgccaagaca cttgagccgt taagcgccaa atacaaaaac atcgcgggtg
   101041 ttgaagaaaa attaacctat accgatacct acgcgcagga aaacgtgact atcgatatgg
   101101 aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caacgtttct gctgaagatg
   101161 ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct ggttttaaag
   101221 aagtgaaata atctgtcggc ggccatgttt cgcatggccg ccatacccgt cttagctttt
   101281 cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca
   101341 ttcgcggaag tttttatgat ccggtgcacg catcatcgcc gtaatttccg gcatcgaaac
   101401 gcggaactgc tgttcggtga ggatcgccag aatgtcatcg gttttcagcg aaaacgcgat
   101461 gcgtaatttt tttagcacga tgttgttatt aatgcgacgt tccggctcca gtgccggagc
   101521 agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgacg acaaaacaat
   101581 gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg cgatctgttc
   101641 cgcggtggct tcgacattac ccagcgccag aatacgcacc aggtcattat tattggcttt
   101701 caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt caggtgttga
   101761 tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt aagcttttca
   101821 gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg gcctggccgt
   101881 tatcttccag acgaatctcc tgtaaatgcg cgaggttaac cagatactga cgatggcagc
   101941 gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc
   102001 cttcgtggct ggtaacgtag acaccgctca tccgactgct gacaaatgcc acatctttca
   102061 tttgcagcaa ataaatccga ctatgcccag tacaagggat aaatttcagc gcctgttgat
   102121 tttctggtaa cagcgaaaca tcctgcttgc tgcgttcctg ccgcaaacgc gccagcgttt
   102181 tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcgtgt tcttcaaagg
   102241 ctttaatggc gtattcgtca aacgcggtga gaaaaacaat atacggacga tgttccgggt
   102301 cgagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg agaaacagca
   102361 catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa cactcaccaa
   102421 cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt
   102481 catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc gtaacgttat
   102541 tcgggtgtaa ctatcaggct cacaggcgac gcttatccca tagtcatcgc caaaccgttc
   102601 acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata
   102661 caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc gggcgctgat
   102721 tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt tttccactat
   102781 cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatgtt
   102841 gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt tcacatgttc
   102901 aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt
   102961 ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cgacggatca ccgctttaat
   103021 ggtgttaagc gcattaaaca aaaaatgggg gttcacctgg gcgtgaagca gtttgatctc
   103081 tgactgggtg agcatcgctt tttgccgctc atattgcccg gcgaggatct gcgccgaaag
   103141 cagttgcgca atcccctcgc ccagcgtgcg gttgattgaa ctgaataaac ggtttttggc
   103201 ttcatacaat ttgatggtgc ccatcacccg ctgattttca ccacgcaacg gaattaccag
   103261 cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt cgttgccatc
   103321 agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag atatcggttt
   103381 gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt
   103441 aatcgcgact gcaccaatat ccagctcctg atacagcacc tgtgccactt tcatgctgtt
   103501 cacttcgtta aacccctggc gcaaaatgcc ttccgtcgag gcagccactt tcagcgcagt
   103561 ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat
   103621 aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat tactcaccag
   103681 acgcaccgca tcttcataag gtcgggcgat cgccaggatg atcaacattt gcaccatttc
   103741 agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag tgcgcccgcg
   103801 acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg tcgagatcat
   103861 gcaacttagc gcggtcatgc cccccatcga atatcgatgt aacccgccgg tcaggccaac
   103921 cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta tcgcacgggt
   103981 attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga
   104041 aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca ttaacggtat
   104101 gaataatggc gttttactca ttaaccatgc aatgactaaa aaaacgcaca tctgctgaag
   104161 cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc tttaaaacgc
   104221 gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat gagaattcgt
   104281 gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctataaat gcacaaaaaa
   104341 cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca
   104401 agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt gatattaacc
   104461 ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa cggacagacg
   104521 gcggtcatcg gctgttcaac gatgccgata ttgaccggat ccgcgagatc aaacgctgga
   104581 tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa aatgttgatg
   104641 tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ttacttgcaa agcggcaatc
   104701 tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc cagacgctca
   104761 ccacgcattt gtttattcct ctgcgtcgac ggcttcagtg ccaacaaccg actctccagg
   104821 cgctgctggc gatcctcgac ggcgtactga tcaactacat cgccatctgt ctggcttcgg
   104881 cacgtaaaaa aaagggtaaa gatacgctgg tggttggctg gaatattcac gataccaccc
   104941 gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat gtcctcgccc
   105001 actcgctcaa tcaactacgc cctgaactgt tcgaaggccg tacattgctg gtgtggtgcg
   105061 gtgaaaatcg aacctccgcc caacaacagc aactcaccag ttggcaagaa caaggctatg
   105121 atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg tacaatcctt
   105181 tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg ccctgttcct
   105241 gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct ttattttgcg
   105301 tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat cgccgccagt
   105361 gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag cccgatgatg
   105421 atgggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt accaatgttg
   105481 ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg tagctcgacc
   105541 ttacgcagtc gctgaccacg actcattccc atacctttcg caacttctgt cacgctggca
   105601 tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg gcagcacacc gtaaaggatc
   105661 aaggcgataa tcgctggttg cagaccaaac ccgatcaccg gaacggcgat cgccagcact
   105721 gcgacgggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg gcgaaattcc
   105781 gcgccccacg ggcgagtgac agcaattccg gcaccagtac caatgatcac cgcaaacaaa
   105841 ctcgaaattc ccaccagcca gaaatgagcc agtgccagag cggcaaaact ttcttgctga
   105901 taaacgggtc gtggcagttg agggaacaag gcagcaaaca gcggctggct gtaaggcagc
   105961 cagaaaatca gtgccacaaa caaagcaatg agccagaaca gcggatcgcg caacatcttc
   106021 atacgcttac gcctcctcca gcagatcctg aaaatgcagc gtgccgctag gctggccctg
   106081 cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca aacagggaga gcgcatcgcg
   106141 cagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt tcttcgcgac gcacgtaatc
   106201 cgccacacta cgtaacgaaa gcaggcgcac acccagttca ctacgtccaa aaaactggcg
   106261 gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttcccctgct gcaccacttc
   106321 accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa tatcatgagt
   106381 gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc gtcatctctt gttgcaacgc
   106441 gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc atcagtaaga cttgcggatc
   106501 ggcagccagt gcgcgcgcta ctcccacacg ttgctgctga ccaccggaaa gctgatgcgg
   106561 ataacgctca cgtaaatttg actccagccc cagtagcgcc attaattcgt cgatacgatc
   106621 gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg gtggcaatgt tttgtgccac
   106681 gctccagtgg ggaaacaggc caatagattg aatggcatag cccatacggc ggcgcaactc
   106741 cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atctctccgc tgtcatgttc
   106801 caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg tgccaatcag
   106861 caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt tttgtgcgcc
   106921 aaacagtttg ctgacatggc taaattcaat cattacgttt caccttcaac agtgcgataa
   106981 gtaaatcgaa cagcgcatcg atcagcaccg ccagaacaat taccgggatc acccccagca
   107041 acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca aaaccgcctg
   107101 cgccgattaa cgccgcaatc accgccatgc ctacagtttg caccatcacc acccgcaggc
   107161 tgcgcagaaa taccggtaac gccagcggta actgaacatg caggaatcgc tgcgccccgc
   107221 tcatccccat cgctctggcg ctctccagca catcgcgcgg gatctggttc aagcctacta
   107281 ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt gcgggtgtca
   107341 ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtcacc agcgcggcaa
   107401 gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg acattgagca
   107461 gagaaaaaat cgccccctgc cgcgcggtgg aaaagtagca ccagatgccc aatggcacac
   107521 caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttga gccagcgcgt
   107581 cgtcaaacac atcctgacgg ttggcgtatt ctttcattag cgagagatcg ttaagcgtgc
   107641 cggagtaaag caaccacagc ggaataatgg caatctgcat atgcaacaac cagcgccaca
   107701 gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc agcgccgcag
   107761 ccagccagaa accactgccg aggctggtac gcgccagcgc actgccattt tgcgccagtt
   107821 gggtcgccgc ctttccagct ccccacacca gcaatacgaa gacgaattgc gccagaatga
   107881 gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc tgtcagccag gcgcaaccaa
   107941 cgcccaccag catccagatc gtttgcggcc acagttgcca gagatgacgc ccttcacccg
   108001 agactaaacg attaggcgcg tagctgataa acggcagcgc tgctgcgatt gccgtcagca
   108061 acagcagcag cgccagaaca ggattaatac gtagataagt cacgggaaat tacttcgccc
   108121 accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct tccactgcaa
   108181 tgctggcatt cagttgctgc aatgtttttg catcgaggct ggcgaagact ggctgtagcc
   108241 actgtgccat ttgcggatac tctttcagca ccgactcacg caccactggt gcaggcgcgt
   108301 agataggttg cacgccttgc ggatcgctta aggtttgcag ccccagcgcc gcgaccggac
   108361 cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt ttgctgggca gcggctttaa
   108421 tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg agcttaaagc
   108481 cataggcttt ttcaaacgcg ggtaaggcat cggcgcgttc gataaactct gccgaggctg
   108541 ccagtttgaa ggtgccgccc tctttcagat aacgactcag gtcagcaagc gaagtgagtt
   108601 tgtttttctc tgccacatcc tgacgcacgg cgatggtcca ggtgttattt gcaggtgcgg
   108661 gcgtcagcca gattaacttg ttttgctctg catcgagttt tttgactttc tcgtaacctt
   108721 gctgcgcgtt tttccacgct gcatcgtttt catctttaaa gaaaaacgcg ccattgccgg
   108781 tatattccgg atagatatcc agttcaccgg aagtaatcgc cccgcgcacc acaggagtcg
   108841 ttccgagttg cactttattg accgtcggta cgccgtggct ttccagtacc tgcaaaatga
   108901 tattgccgag cagcgcgcct tcagtatcga tttttgaacc gactttaacg ggggaagccg
   108961 cgtgcagcgg caggctcacg gctgccaaca aaaccagtga acctgcccag acctttgaga
   109021 gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag agaaaagcat
   109081 agttgataat ggcgggatta gcctgagcgg acggattcag ttgcagaatc agataaatac
   109141 ttaagaggca tattcggtcc ggcgtttcct gccggatgcg gcgcgagcgc cttatccggc
   109201 ctacaaaggg cgaaaattca gcaagttaag atatgaacat caggcaaagg gcgcaaatgt
   109261 cacgccctta ctaaagcatt acagcaactc aaactcgcct ttcttaacgc gtgcggaatc
   109321 agtgccgata aagacattga acttgccagg ctcggcgtca tatttcatct gttgattcca
   109381 gaacttcagc gcctcgatat caatcgggaa gctaacggtc tgagtttcgc ccggtttcag
   109441 ggtgattttc tcaaagcctt tcagctgttt caccggacga ctcatggaag ccgtcacatc
   109501 ctgcaagtac atctgcacta ccgtggcccc ttcgcgttta ccggtgttcg tcacctgcac
   109561 gctggccgtc actttgccgt cacgcttcat ggtcggcgca gaaagtttca catcagagac
   109621 ggtgaaagtg gtatagctca gaccatagcc gaacggataa agcgccccgt tagcttcatc
   109681 aaaataacgc gaagtgtatt tgttcggctt gtcggcatta tacggacgac cggtgttcag
   109741 atggctgtag tacaccggga tctgcccgac agaacgcggg aaggacatcg gcagcttgcc
   109801 ggacgggttg taatcgccaa acaacacatc ggcaattgca ttaccgcctt cagtccccgc
   109861 aaaccaggtt tccagaatcg catcagcctg ctgatcttct ttcaccagcg ccagcggacg
   109921 cccgttcatc agcaccagca ccagcggttt accggtggct ttcagcgccg caatcaagtc
   109981 acgttggctt tgcggaatag taatatcggt acggctggag gcctcgtgcg ccatcccctg
   110041 tgcttcaccg actacagcca ccaccacatc agattgtttc gccgtctgca ccgcttcatc
   110101 aatcatctct tgcggcgaac gcggatcgac tttgaccgct tcttcatact gattcaggaa
   110161 atcgataatg cctttgtcac tggtaacgtt cgcccctttg gcatacagca ctttaccgtt
   110221 ttcaccgacg gcatttttaa tcccggtcag cacggtcacg gattgatctg caacacctgc
   110281 cgccgaccag cttcccatca cgtcacgctt gctgtctgcc agcgggccaa ccaccgcaat
   110341 ggttgcggat tttttcagcg gtaacgtttc gagacggttt ttcagcaaca ccaggctttc
   110401 acgcgccact tcacgcgctt ctttgcggtg caagcggctt tcggcattgg tatccaccgg
   110461 gtcagactct ttcggaccga gatggctgta tgggtcgtta aacaacccca tatcatattt
   110521 aacgttcagt acatggcggg cagcatcgtc cagctctgcc atcgtcactt taccggattt
   110581 aatcaaccca ggcagatact tcgagtagta ctcgtcgctc atactcatgt tgattccgga
   110641 tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc gtgccatgtt taatcagctc
   110701 tttgattgca ccgtgatcgg aaacggtgat gcctttaaag ccccactggt cgcgcagaac
   110761 atctttcagc agccaggaat cggaggtggc tggcgtgccg ttcagcgagt tcagcgccac
   110821 catcaccgcg ccgctgcctg cgtccagccc cgctttgtac ggcggcatat aatcattaaa
   110881 caggcgctgc ggactcatat cgacggtgtt gtactcttta ccgccttcta ccgcaccgta
   110941 tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga tctgccgggc ttttaccctg
   111001 catcgcttcc accatggttt tacccattat tgaggtgaga tacgtatctt cgccaaaacc
   111061 ttcggaagca cgtccccagc gcggatcgcg cgagacatcg accatcggtg cccaggtcat
   111121 attcaggcca tcatctgccg cttcataagc agagacacgt ccgaccgttt tcactgcatc
   111181 gaggttaaaa gacgaggcca gaccgaggct aatcgggaac accgtgcgct gaccgtgcag
   111241 cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt aattccatca cctgatcctg
   111301 catggcgcgg atatcctgac gggttacggt gttgaaaatc gccccaacct gaccgtcttt
   111361 gatcatctcg cggatcgcct ctttcgggtt atccgggccg acgctgatta agcgcagctg
   111421 accaattttc tcatcaactg tcattttctt aagcagttcg gtgacgaacg catcccgcgc
   111481 ttcgggcgtt aatggatggt tgccgaataa atcatccgcc agtgctggct gcagggccag
   111541 actcaccgcg attcctactg aacatagcca tttcatatgg atttttcttc ctttgttgcc
   111601 cgacgtggca gcgaaaatgg tgcaaaaacc gtagtttgcc ataagcatga tggagagaga
   111661 aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt cactcgctga attgttatac
   111721 aaggcgctat tctagtttgt gatatttttt cgccaccaca aggagtggaa aatgtcttcc
   111781 atgacaacaa ctgataataa agcctttttg aatgaacttg ctcgtctggt gggttcttca
   111841 cacctgctca ccgatcccgc aaaaacggcc cgctatcgca agggcttccg ttctggtcag
   111901 ggcgacgcgc tggctgtcgt tttccctggc tcactactag aattgtggcg ggtgctgaaa
   111961 gcctgcgtca ccgccgacaa aattattctg atgcaggccg ccaatacagg cctgaccgaa
   112021 ggatcgacgc caaacggtaa cgattatgat cgcgatgtcg ttatcatcag caccctgcgt
   112081 ctcgacaagc tgcacgttct tggcaagggc gaacaggtgc tggcctatcc gggcaccacg
   112141 ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg aaccgcactc agtgattgga
   112201 tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta acaactccgg cggctcgctg
   112261 gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg cgcgtataaa tgaagacggc
   112321 aaactgacgc tggtgaacca tctggggatt gatctgggcg aaacgccgga gcagatcctt
   112381 agcaagctgg atgatgatcg catcaaagat gacgatgtgc gtcacgatgg tcgtcacgcc
   112441 cacgattatg actatgtcca ccgcgttcgt gatattgaag ccgacacgcc cgcacgttat
   112501 aacgccgatc ctgatcggtt atttgaatct tctggttgcg ccgggaagct ggcggtcttt
   112561 gcagtacgtc ttgatacctt cgaagcggaa aaaaatcagc aggtgtttta tatcggcacc
   112621 aaccagccgg aagtgctgac cgaaatccgc cgtcatattc tggctaactt cgaaaatctg
   112681 ccggttgccg gggaatatat gcaccgggat atctacgata ttgcggaaaa atacggcaaa
   112741 gacaccttcc tgatgattga taagttaggc accgacaaga tgccgttctt ctttaatctc
   112801 aagggacgca ccgatgcgat gctggagaaa gtgaaattct tccgtccgca ttttactgac
   112861 cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt taccgccgcg catgaaaaac
   112921 tggcgcgata aatacgagca tcatctgctg ttaaaaatgg cgggcgatgg cgtgggcgaa
   112981 gccaaatcgt ggctggtgga ttatttcaaa caggccgaag gcgatttctt tgtctgtacg
   113041 ccggaggaag gcagcaaagc gtttttacac cgtttcgccg ctgcgggcgc agcaattcgt
   113101 tatcaggcgg tgcattccga tgaagtcgaa gacattctgg cgttggatat cgctctgcgg
   113161 cgtaacgaca ccgagtggta tgagcattta ccgccggaga tcgacagcca gctggtgcac
   113221 aagctctatt acggccattt tatgtgctat gtcttccatc aggattacat agtgaaaaaa
   113281 ggcgtggatg tgcatgcgtt aaaagaacag atgctggaac tgctacagca gcgcggcgcg
   113341 cagtaccctg ccgagcataa cgtcggtcat ttgtataaag caccggagac gttgcagaag
   113401 ttctatcgcg agaacgatcc gaccaacagc atgaatccgg ggatcggtaa aaccagtaaa
   113461 cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg cagagtatcg ccatccgaat
   113521 tcacttaatc gttctgtgcc gtctgccccg ccgccgccat ttgggcggct ttttgttttt
   113581 tatagctcaa cgctgctgcc ggcacaggca tcactttacc ggtttcaatc caggtacgca
   113641 ggcggctggc atcggcaaaa tgggtatatt tgccaaacgc gtccatcact accagcgcca
   113701 ccggtttatt attgataacc gtacgcatca ccagacaatg gcccgccgca ttggtaaagc
   113761 cggttttggt taactgaata ttccagttat cgcgatacac cagatgatta gtattgcgga
   113821 acggcagcgt atacgtcgga ttagagaagg ttgccatatc ttcccgggta gtacttaact
   113881 gcccgatcaa cggatattgt ttgctggcaa tgagcagttt ggttaagtca cgggcagttg
   113941 aaacgttatg caccgacaat ccggtaggtt caacaaagcg cgtgttgttc attccgagcg
   114001 atttcgcttt cgcattcatt gccttaataa aggctttgta accaccggga taatggtgcg
   114061 caaggcttgc cgccgcgcgg ttttctgaag acatcagcgc cagcaacagc atatctttac
   114121 ggctgatttc gctattcagt cgtacgcgcg aatagacccc tttcatctcc ggcgtctggc
   114181 tgatatccac ttttagtttt tcatccagcg gcagtcgtgc atccagcaca accatcgcgg
   114241 tcattaattt gctgatagac gcaatcggac gcaccagatc cgggtggttc gaatagatca
   114301 ctttgttggt attcagatca acaatcatcg cgctaccgga ggcaatttcc ggttgtgaag
   114361 cggtggtagc ggctgccgtt ttcgcaacgg cctgcggtgc aaaaggcaca gccagcatca
   114421 gggccaggct aaataaagaa actcgaaatt tcggcatgat gagcattcag atagtggttc
   114481 acgcgcacgg gttgcgcacc gccggagtaa ggatttactg aggctagcga cgccatcata
   114541 acgagcaaaa agtgcgatcg tcaaaggaga atcgtgagga aatgctgcat tgctgacatt
   114601 tacgccagca atgcaacgtc aaagaacttt ctagaacaaa cgataaccgt agccccatag
   114661 tataacggtt agggcgagca gcacttccag taccagcacg ccaatcgcca gcgtcgaact
   114721 agagaagcta aggccttcct ctttgttgat attcaggaag ctcggaatac caaggtaaag
   114781 caggtagccg gtgtaaaaca gcgccaccgt gccgaccagc gcacacaacc agaccagtgg
   114841 ataaagcgcc accagaccgc ttaaaaacag cggagttgca acgtagcccg cgaagaccat
   114901 gcagtgcgca agtgacggac gctgcggata attacgcgcc atccaccaga tgacccgccc
   114961 catcaccgcg accccagcca gcataacgcc gtaaaacaag acagccagcg ccagtccggt
   115021 aaaccaggat aacttcagga tagtgccatc gccaaaattc cagccaatct gtgtagtgcc
   115081 aatgaaggcg caaatcaccg gaatcgccgc catcagcaaa acgtggtggg tgtaatggtg
   115141 agaaatcgtt tcgttttcgc gattaatcac ctgcatttca cgatcgggat gggaaaacag
   115201 tccccagaca tggctcatac cgcccccttg ttgtgagttc atgaacctga cagttcaagt
   115261 ataagtcagc ttgtgattat tttttgttcg cccatgtaat tttcactgtc tgatgaacgt
   115321 cctttttcag cgtgaatgat tcacagggtg tatgcttact ggcaaccaaa gggagacaga
   115381 ctggcctatg gatctcaata cacttatctc acaatatggt tatgccgcgc tggtgatcgg
   115441 tagcctggcg gaaggtgaaa ccgtgacttt gctgggaggc gttgcggcgc atcaggggct
   115501 attaaagttc ccgctggtgg tactttctgt ggcgcttggc ggcatgattg gcgaccaggt
   115561 gctctatctg tgcgggcggc ggtttggcgg caagctgtta cgccgtttct cgaaacatca
   115621 ggataaaatt gagcgggcgc agaaacttat ccaacgccat ccgtatctgt ttgtcattgg
   115681 tacgcgcttt atgtatggct ttcgggtgat tggcccgacg ctgattggtg ccagccagct
   115741 gccgccgaaa atctttctgc cgctgaatat tctcggcgca tttgcctggg cgttgatttt
   115801 taccactatt ggttacgctg gtggtcaggt gattgcgccg tggttgcaca atctcgacca
   115861 gcatttgaag cactgggtct ggttgattct ggtcgtggtt ctggtggtgg gcgtgcgctg
   115921 gtggctgaaa cgacgcggga agaaaaagcc ggatcatcag gcgtaaaacc attgccctgg
   115981 ataaggcgtt cgcgccgcat ccgacatccg gagtaccatg cctgatacga cgcttgtgcg
   116041 tcttttcatg ccgaccaaaa tattacccaa tgaaatatcc acgcacctca ctgcgctatt
   116101 ctgggttgaa ctgtggattc gccaacataa agccgccatc cactatcaac gactgcccgg
   116161 tggtgtaatt tgcgccctcc gaacaaagcc acaccaccag gctggcaatc tcatgcgttg
   116221 cgccaaaacg ccgcaaggga atcgaaggct ccgcgtcggg cttcacgtcg ctgtcatcca
   116281 tgccattcat tggcgtggcg atcgccccag gcgcgactgc gttcaccaaa atcttatgcc
   116341 tgaccagctc cagcgccatc gctttggtta acccaccgag cgcatgttta gcggctgtgt
   116401 aggcgctggc atccggcagc ggcgtatgtt catgtaccga cgtaatgttg atgatgcgac
   116461 cgccctgccc ttgtttcacc atctgacgag ccgcaatttg cgagcataag aatgcaccat
   116521 cgacatcaac ggtaaaaatc ttgcgccact catcaaaagc catatcaaga aacggcgctt
   116581 tggtcattgc acccgcatta ttcaccagca catcaatgcg ccccagccgt tgaatgagtt
   116641 tctccagcgc cagtgcccct tctggtagat tgccgagatc cagctgcacg atctccgcac
   116701 gtacgccgtg gctaactacc tcacgcgcga tatcttttgc cccttcttca tctgagtgcc
   116761 aggtaatacc aatatcaaac ccctgctgcg ccagtaataa cgcgcactct ttgccgatcc
   116821 ccgaatcgga ggcggtaata atcgcaacct gtgccatcga gttctccact taacgctgaa
   116881 taaacgttaa gtatagaagg cgcatatcat cagcgtttgt aacccccgcc caacgcacca
   116941 gtgagttgaa tggaggcatc cagccactgc ccttgcaata acaggccatt ggcccgctca
   117001 cgcagcgcgg ggattctggc ttcgctgacg cgggaaccag caatgatgcc cgcgttaaag
   117061 cgcgcctgcg caagacctac cacacgcaag gcatcgcgct caatttgcgc ctgatgctgg
   117121 tttttctccg ccagtgtctg aacctgactg gctgcccgcg ccacgtcatt caccgcttca
   117181 accaccgctt tgttgtagct ggcgatagac aagttgcttt cggcttttgc gatatcgaga
   117241 ttggcgttaa gacgaccact atcgaaaatg ggtagcgtca ggcctgccgt aacgcccatt
   117301 tgctgcgcgg aatgacggaa cagatcgctt aagtgcaacg catcctgttg caggaaggcc
   117361 atcaggttga tgtcaggata aaatgccgct tttgccgcat caatggtgct tagcgatgac
   117421 tcaacgtacc agtgcgccgc ctgcaaatct gcccgccggg ccagtaagga gtaccccagt
   117481 tcatcaggaa gctggcttgc cactttcggc aacgcgaccg gtttaagctt caatgacttt
   117541 gtctggttat ttgtaagtgc gcttaaccgt gcctcaataa ttttcatttt ccccgcgaca
   117601 tcgttgagct gctgccgggt tttgctggca ttaatatcgg tttccacacc ttcaactgaa
   117661 gaagtaatcc cgttctgata tagctggcga tcggtcgcga taatggtgtt ctgctctttt
   117721 tctatttgct gcaagaccgt gtttaacgcc gcctgggttt gccactccca gtacaggcgg
   117781 gctacgctgc cagccagcaa ttgacgggtt tgctcgcgtt ccgccgcccg tgctttaacc
   117841 gtacccaggc gggcagtaac ctccgcccga ttctttcccc agatatcgag atgccagccc
   117901 gccgttaagc caaaagtacc gttggtgtac cacgggccgg tcgtacctgc ggccggatcg
   117961 ttcagagcaa acggccccat taagccttct gccgacattt tttgccgctc catatccgcc
   118021 gaaaagtcga tctgcggacc atcctgagtg gcaactgcct tcgcctgggc ttcagctagc
   118081 tgaatgcgct gttcagccac ctgcatatcc ggtgcgttct gtagtgcatt gttaattaag
   118141 gaagtgagtt gattatcgtg atactccagc caccattggc tgtctggcca accatttttc
   118201 agcgccgtgg gtaatgcggt gtcaacttgt gcagcgggcg tttgctggct taacgcctgg
   118261 cgggtttcat gcataggcgc acacccggcc agcatcagta acagcggaaa acaggcgatg
   118321 gctggataaa aggaatcacg attcatgggg gaataatcag gtaagaaaag gtgcgcggag
   118381 attaccgtgt gttgcgatat attttttagt ttcgcgtggc aatacatcag tggcaataaa
   118441 acgacatatc cagaaaaata tacactaagt gaatgatatc ttccgattta tcttaatcgt
   118501 ttatggataa cggcaaaggg cttcgttttt tcctatactt attcagcact cacaaataaa
   118561 ggaacgccaa tgaaaattat actctgggct gtattgatta ttttcctgat tgggctactg
   118621 gtggtgactg gcgtatttaa gatgatattt taaaattaat taatgtcatc aggtccgaaa
   118681 ataacgagaa tatttcagtc tctcatcctg ttgcgctcct gtcatgtgca ttgcttcata
   118741 taatcactgg cgcaaggagc gcgcaggggg cggccaatcg ccgccgcccc ctgcaccccc
   118801 gggctctggc gaacaaaatc gccgctgcgc ggtgccctcg acttatccct tacggctac
//
LOCUS       DAHBHC010000003       117304 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHBHC010000003 DAHBHC010000000
VERSION     DAHBHC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.003"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(2..94)
                     /locus_tag="M2A35_000228"
     tRNA            complement(2..94)
                     /locus_tag="M2A35_000228"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="M2A35_000229"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="M2A35_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCB7196580.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="M2A35_000230"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="M2A35_000230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCB7196581.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="M2A35_000231"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="M2A35_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCB7196582.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="M2A35_000232"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="M2A35_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCB7196583.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="M2A35_000233"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="M2A35_000233"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCB7196584.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="M2A35_000234"
     CDS             complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="M2A35_000234"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCB7196585.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7454..8017
                     /gene="srlA"
                     /locus_tag="M2A35_000235"
                     /gene_synonym="gutA"
     CDS             7454..8017
                     /gene="srlA"
                     /locus_tag="M2A35_000235"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCB7196586.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="M2A35_000236"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="M2A35_000236"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCB7196587.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="M2A35_000237"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="M2A35_000237"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCB7196588.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="M2A35_000238"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="M2A35_000238"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCB7196589.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="M2A35_000239"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="M2A35_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCB7196590.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="M2A35_000240"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="M2A35_000240"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCB7196591.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELVQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="M2A35_000241"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="M2A35_000241"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCB7196592.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKITATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="M2A35_000242"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="M2A35_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCB7196593.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="M2A35_000243"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="M2A35_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCB7196594.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="M2A35_000244"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="M2A35_000244"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCB7196595.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16893..19145)
                     /gene="hypF"
                     /locus_tag="M2A35_000245"
     CDS             complement(16893..19145)
                     /gene="hypF"
                     /locus_tag="M2A35_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCB7196596.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19298..19825)
                     /gene="hydN"
                     /locus_tag="M2A35_000246"
     CDS             complement(19298..19825)
                     /gene="hydN"
                     /locus_tag="M2A35_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCB7196597.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19974..20984)
                     /gene="ascG"
                     /locus_tag="M2A35_000247"
     CDS             complement(19974..20984)
                     /gene="ascG"
                     /locus_tag="M2A35_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCB7196598.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21244..22701
                     /gene="ascF"
                     /locus_tag="M2A35_000248"
     CDS             21244..22701
                     /gene="ascF"
                     /locus_tag="M2A35_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCB7196599.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            22710..24134
                     /gene="ascB"
                     /locus_tag="M2A35_000249"
     CDS             22710..24134
                     /gene="ascB"
                     /locus_tag="M2A35_000249"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCB7196600.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     KITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24248..24667)
                     /locus_tag="M2A35_000250"
     CDS             complement(24248..24667)
                     /locus_tag="M2A35_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7196601.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24738..25492)
                     /locus_tag="M2A35_000251"
                     /pseudo
     CDS             complement(24738..25492)
                     /locus_tag="M2A35_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25535..26005)
                     /gene="hycI"
                     /locus_tag="M2A35_000252"
     CDS             complement(25535..26005)
                     /gene="hycI"
                     /locus_tag="M2A35_000252"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCB7196602.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(25998..26408)
                     /gene="hycH"
                     /locus_tag="M2A35_000253"
     CDS             complement(25998..26408)
                     /gene="hycH"
                     /locus_tag="M2A35_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCB7196603.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26405..27172)
                     /gene="hycG"
                     /locus_tag="M2A35_000254"
     CDS             complement(26405..27172)
                     /gene="hycG"
                     /locus_tag="M2A35_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCB7196604.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27172..27714)
                     /gene="hycF"
                     /locus_tag="M2A35_000255"
     CDS             complement(27172..27714)
                     /gene="hycF"
                     /locus_tag="M2A35_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCB7196605.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27724..29433)
                     /gene="hycE"
                     /locus_tag="M2A35_000256"
     CDS             complement(27724..29433)
                     /gene="hycE"
                     /locus_tag="M2A35_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCB7196606.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(29451..30374)
                     /gene="hycD"
                     /locus_tag="M2A35_000257"
     CDS             complement(29451..30374)
                     /gene="hycD"
                     /locus_tag="M2A35_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCB7196607.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30377..32203)
                     /gene="hycC"
                     /locus_tag="M2A35_000258"
     CDS             complement(30377..32203)
                     /gene="hycC"
                     /locus_tag="M2A35_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCB7196608.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32200..32811)
                     /gene="hycB"
                     /locus_tag="M2A35_000259"
     CDS             complement(32200..32811)
                     /gene="hycB"
                     /locus_tag="M2A35_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCB7196609.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32936..33397)
                     /gene="hycA"
                     /locus_tag="M2A35_000260"
     CDS             complement(32936..33397)
                     /gene="hycA"
                     /locus_tag="M2A35_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCB7196610.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33610..33960
                     /gene="hypA"
                     /locus_tag="M2A35_000261"
     CDS             33610..33960
                     /gene="hypA"
                     /locus_tag="M2A35_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCB7196611.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33964..34836
                     /gene="hypB"
                     /locus_tag="M2A35_000262"
     CDS             33964..34836
                     /gene="hypB"
                     /locus_tag="M2A35_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCB7196612.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34827..35099
                     /gene="hypC"
                     /locus_tag="M2A35_000263"
     CDS             34827..35099
                     /gene="hypC"
                     /locus_tag="M2A35_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCB7196613.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35099..36220
                     /gene="hypD"
                     /locus_tag="M2A35_000264"
     CDS             35099..36220
                     /gene="hypD"
                     /locus_tag="M2A35_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCB7196614.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36217..37227
                     /gene="hypE"
                     /locus_tag="M2A35_000265"
     CDS             36217..37227
                     /gene="hypE"
                     /locus_tag="M2A35_000265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCB7196615.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37301..39379
                     /gene="flhA"
                     /locus_tag="M2A35_000266"
     CDS             37301..39379
                     /gene="flhA"
                     /locus_tag="M2A35_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCB7196616.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(39416..39769)
                     /locus_tag="M2A35_000267"
     CDS             complement(39416..39769)
                     /locus_tag="M2A35_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCB7196617.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(39801..39980)
                     /locus_tag="M2A35_000268"
     CDS             complement(39801..39980)
                     /locus_tag="M2A35_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196618.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            40056..42617
                     /gene="mutS"
                     /locus_tag="M2A35_000269"
     CDS             40056..42617
                     /gene="mutS"
                     /locus_tag="M2A35_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCB7196619.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42723..43379
                     /gene="pphB"
                     /locus_tag="M2A35_000270"
     CDS             42723..43379
                     /gene="pphB"
                     /locus_tag="M2A35_000270"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCB7196620.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(43430..44197)
                     /locus_tag="M2A35_000271"
     CDS             complement(43430..44197)
                     /locus_tag="M2A35_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCB7196621.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            44393..45301
                     /locus_tag="M2A35_000272"
     CDS             44393..45301
                     /locus_tag="M2A35_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCB7196622.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGEHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            45298..46560
                     /locus_tag="M2A35_000273"
     CDS             45298..46560
                     /locus_tag="M2A35_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCB7196623.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            46557..47195
                     /locus_tag="M2A35_000274"
     CDS             46557..47195
                     /locus_tag="M2A35_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCB7196624.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            47200..47976
                     /locus_tag="M2A35_000275"
     CDS             47200..47976
                     /locus_tag="M2A35_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCB7196625.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            48065..49429
                     /locus_tag="M2A35_000276"
     CDS             48065..49429
                     /locus_tag="M2A35_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCB7196626.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     SVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(49468..49704)
                     /locus_tag="M2A35_000277"
     CDS             complement(49468..49704)
                     /locus_tag="M2A35_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196627.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYREAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(49715..51142)
                     /locus_tag="M2A35_000278"
     CDS             complement(49715..51142)
                     /locus_tag="M2A35_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCB7196628.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(51142..51735)
                     /locus_tag="M2A35_000279"
     CDS             complement(51142..51735)
                     /locus_tag="M2A35_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCB7196629.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51882..52289
                     /locus_tag="M2A35_000280"
     CDS             51882..52289
                     /locus_tag="M2A35_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCB7196630.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(52410..53402)
                     /gene="rpoS"
                     /locus_tag="M2A35_000281"
     CDS             complement(52410..53402)
                     /gene="rpoS"
                     /locus_tag="M2A35_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCB7196631.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVVSRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(53465..54604)
                     /gene="nlpD"
                     /locus_tag="M2A35_000282"
     CDS             complement(53465..54604)
                     /gene="nlpD"
                     /locus_tag="M2A35_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCB7196632.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54744..55370)
                     /gene="pcm"
                     /locus_tag="M2A35_000283"
     CDS             complement(54744..55370)
                     /gene="pcm"
                     /locus_tag="M2A35_000283"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCB7196633.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(55364..56125)
                     /gene="surE"
                     /locus_tag="M2A35_000284"
     CDS             complement(55364..56125)
                     /gene="surE"
                     /locus_tag="M2A35_000284"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCB7196634.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(56106..57155)
                     /gene="truD"
                     /locus_tag="M2A35_000285"
     CDS             complement(56106..57155)
                     /gene="truD"
                     /locus_tag="M2A35_000285"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCB7196635.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(57152..57631)
                     /gene="ispF"
                     /locus_tag="M2A35_000286"
     CDS             complement(57152..57631)
                     /gene="ispF"
                     /locus_tag="M2A35_000286"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCB7196636.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(57631..58341)
                     /gene="ispD"
                     /locus_tag="M2A35_000287"
     CDS             complement(57631..58341)
                     /gene="ispD"
                     /locus_tag="M2A35_000287"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCB7196637.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(58360..58671)
                     /gene="ftsB"
                     /locus_tag="M2A35_000288"
     CDS             complement(58360..58671)
                     /gene="ftsB"
                     /locus_tag="M2A35_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCB7196638.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58865..59188)
                     /locus_tag="M2A35_000289"
     CDS             complement(58865..59188)
                     /locus_tag="M2A35_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCB7196639.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(59238..59843)
                     /gene="cysC"
                     /locus_tag="M2A35_000290"
     CDS             complement(59238..59843)
                     /gene="cysC"
                     /locus_tag="M2A35_000290"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCB7196640.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHCHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59843..61270)
                     /gene="cysN"
                     /locus_tag="M2A35_000291"
     CDS             complement(59843..61270)
                     /gene="cysN"
                     /locus_tag="M2A35_000291"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCB7196641.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(61272..62180)
                     /gene="cysD"
                     /locus_tag="M2A35_000292"
     CDS             complement(61272..62180)
                     /gene="cysD"
                     /locus_tag="M2A35_000292"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCB7196642.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            62432..63469
                     /gene="iap"
                     /locus_tag="M2A35_000293"
     CDS             62432..63469
                     /gene="iap"
                     /locus_tag="M2A35_000293"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCB7196643.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   63551..63945
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=63612..63640
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(64042..64335)
                     /gene="cas2"
                     /locus_tag="M2A35_000294"
     CDS             complement(64042..64335)
                     /gene="cas2"
                     /locus_tag="M2A35_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCB7196644.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(64335..65255)
                     /gene="cas1e"
                     /locus_tag="M2A35_000295"
     CDS             complement(64335..65255)
                     /gene="cas1e"
                     /locus_tag="M2A35_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCB7196645.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(65252..65902)
                     /gene="cas6e"
                     /locus_tag="M2A35_000296"
     CDS             complement(65252..65902)
                     /gene="cas6e"
                     /locus_tag="M2A35_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCB7196646.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(65884..66630)
                     /gene="cas5e"
                     /locus_tag="M2A35_000297"
     CDS             complement(65884..66630)
                     /gene="cas5e"
                     /locus_tag="M2A35_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCB7196647.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(66641..67714)
                     /gene="cas7e"
                     /locus_tag="M2A35_000298"
     CDS             complement(66641..67714)
                     /gene="cas7e"
                     /locus_tag="M2A35_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCB7196648.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPGFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            complement(67729..68262)
                     /gene="casB"
                     /locus_tag="M2A35_000299"
     CDS             complement(67729..68262)
                     /gene="casB"
                     /locus_tag="M2A35_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCB7196649.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(68259..69821)
                     /gene="casA"
                     /locus_tag="M2A35_000300"
     CDS             complement(68259..69821)
                     /gene="casA"
                     /locus_tag="M2A35_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCB7196650.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(69919..72618)
                     /locus_tag="M2A35_000301"
     CDS             complement(69919..72618)
                     /locus_tag="M2A35_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCB7196651.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLI
                     GSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQ
                     QEAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
     gene            complement(72812..72964)
                     /locus_tag="M2A35_000302"
     CDS             complement(72812..72964)
                     /locus_tag="M2A35_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCB7196652.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(73229..73963)
                     /gene="cysH"
                     /locus_tag="M2A35_000303"
     CDS             complement(73229..73963)
                     /gene="cysH"
                     /locus_tag="M2A35_000303"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCB7196653.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(74037..75749)
                     /gene="cysI"
                     /locus_tag="M2A35_000304"
     CDS             complement(74037..75749)
                     /gene="cysI"
                     /locus_tag="M2A35_000304"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCB7196654.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(75749..77548)
                     /gene="cysJ"
                     /locus_tag="M2A35_000305"
     CDS             complement(75749..77548)
                     /gene="cysJ"
                     /locus_tag="M2A35_000305"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCB7196655.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVAQHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            77867..78229
                     /gene="queD"
                     /locus_tag="M2A35_000306"
     CDS             77867..78229
                     /gene="queD"
                     /locus_tag="M2A35_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCB7196656.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKEICTAGCIYRGE"
     gene            78307..79578
                     /locus_tag="M2A35_000307"
     CDS             78307..79578
                     /locus_tag="M2A35_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCB7196657.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            79569..79829
                     /locus_tag="M2A35_000308"
     CDS             79569..79829
                     /locus_tag="M2A35_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCB7196658.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            79846..80421
                     /locus_tag="M2A35_000309"
     CDS             79846..80421
                     /locus_tag="M2A35_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCB7196659.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(80569..81429)
                     /locus_tag="M2A35_000310"
     CDS             complement(80569..81429)
                     /locus_tag="M2A35_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCB7196660.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(81426..82205)
                     /locus_tag="M2A35_000311"
     CDS             complement(81426..82205)
                     /locus_tag="M2A35_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCB7196661.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(82183..83592)
                     /locus_tag="M2A35_000312"
     CDS             complement(82183..83592)
                     /locus_tag="M2A35_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7196662.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMKLTPFMAGMIGGSAPLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGISLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFDVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(83614..85068)
                     /locus_tag="M2A35_000313"
     CDS             complement(83614..85068)
                     /locus_tag="M2A35_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCB7196663.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(85138..85923)
                     /locus_tag="M2A35_000314"
     CDS             complement(85138..85923)
                     /locus_tag="M2A35_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7196664.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEVAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            86242..87519
                     /locus_tag="M2A35_000315"
     CDS             86242..87519
                     /locus_tag="M2A35_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7196665.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            87546..89024
                     /locus_tag="M2A35_000316"
     CDS             87546..89024
                     /locus_tag="M2A35_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCB7196666.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   89662..89815
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=89665..89692
                     /rpt_unit_seq="ggtttatccccgctggcgcggggaactc"
     gene            complement(90154..90825)
                     /gene="queE"
                     /locus_tag="M2A35_000317"
     CDS             complement(90154..90825)
                     /gene="queE"
                     /locus_tag="M2A35_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCB7196667.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            90964..91104
                     /locus_tag="M2A35_000318"
     CDS             90964..91104
                     /locus_tag="M2A35_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196668.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            91118..91990
                     /locus_tag="M2A35_000319"
                     /pseudo
     CDS             91118..91990
                     /locus_tag="M2A35_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(92050..93348)
                     /gene="eno"
                     /locus_tag="M2A35_000320"
     CDS             complement(92050..93348)
                     /gene="eno"
                     /locus_tag="M2A35_000320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCB7196669.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(93436..95073)
                     /gene="pyrG"
                     /locus_tag="M2A35_000321"
     CDS             complement(93436..95073)
                     /gene="pyrG"
                     /locus_tag="M2A35_000321"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCB7196670.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPECKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(95301..96092)
                     /gene="mazG"
                     /locus_tag="M2A35_000322"
     CDS             complement(95301..96092)
                     /gene="mazG"
                     /locus_tag="M2A35_000322"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCB7196671.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(96163..96498)
                     /gene="mazF"
                     /locus_tag="M2A35_000323"
     CDS             complement(96163..96498)
                     /gene="mazF"
                     /locus_tag="M2A35_000323"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCB7196672.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(96498..96746)
                     /gene="mazE"
                     /locus_tag="M2A35_000324"
     CDS             complement(96498..96746)
                     /gene="mazE"
                     /locus_tag="M2A35_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCB7196673.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(96824..99058)
                     /locus_tag="M2A35_000325"
     CDS             complement(96824..99058)
                     /locus_tag="M2A35_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCB7196674.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(99120..100415)
                     /gene="rlmD"
                     /locus_tag="M2A35_000326"
                     /pseudo
     CDS             complement(99120..100415)
                     /gene="rlmD"
                     /locus_tag="M2A35_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            100472..103228
                     /gene="barA"
                     /locus_tag="M2A35_000327"
     CDS             100472..103228
                     /gene="barA"
                     /locus_tag="M2A35_000327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCB7196675.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(103460..104800)
                     /gene="gudD"
                     /locus_tag="M2A35_000328"
     CDS             complement(103460..104800)
                     /gene="gudD"
                     /locus_tag="M2A35_000328"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCB7196676.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(104821..106161)
                     /locus_tag="M2A35_000329"
     CDS             complement(104821..106161)
                     /locus_tag="M2A35_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCB7196677.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(106163..107515)
                     /gene="gudP"
                     /locus_tag="M2A35_000330"
     CDS             complement(106163..107515)
                     /gene="gudP"
                     /locus_tag="M2A35_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCB7196678.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(107950..108399)
                     /locus_tag="M2A35_000331"
     CDS             complement(107950..108399)
                     /locus_tag="M2A35_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCB7196679.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(108417..109199)
                     /gene="truC"
                     /locus_tag="M2A35_000332"
     CDS             complement(108417..109199)
                     /gene="truC"
                     /locus_tag="M2A35_000332"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCB7196680.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(109199..109528)
                     /locus_tag="M2A35_000333"
     CDS             complement(109199..109528)
                     /locus_tag="M2A35_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCB7196681.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(110150..110695)
                     /gene="syd"
                     /locus_tag="M2A35_000334"
     CDS             complement(110150..110695)
                     /gene="syd"
                     /locus_tag="M2A35_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCB7196682.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            110763..111611
                     /gene="queF"
                     /locus_tag="M2A35_000335"
     CDS             110763..111611
                     /gene="queF"
                     /locus_tag="M2A35_000335"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCB7196683.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            111723..113087
                     /gene="ppnN"
                     /locus_tag="M2A35_000336"
                     /gene_synonym="ygdH"
     CDS             111723..113087
                     /gene="ppnN"
                     /locus_tag="M2A35_000336"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCB7196684.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            113644..114933
                     /gene="sdaC"
                     /locus_tag="M2A35_000337"
     CDS             113644..114933
                     /gene="sdaC"
                     /locus_tag="M2A35_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCB7196685.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            114991..116358
                     /gene="sdaB"
                     /locus_tag="M2A35_000338"
     CDS             114991..116358
                     /gene="sdaB"
                     /locus_tag="M2A35_000338"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCB7196686.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            116470..117225
                     /gene="xni"
                     /locus_tag="M2A35_000339"
     CDS             116470..117225
                     /gene="xni"
                     /locus_tag="M2A35_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCB7196687.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
BASE COUNT        28062 a        31534 c        29433 g        28275 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcctacac ttttatcgtt tgcacacttc acgttcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atcaacagaa aaatggaggt ctgacagata gtagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggtgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggacgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctgg aattcagcct
     4621 gtttaaacgg cgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcgcctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttca
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc tcttgtaaat agctactgta
     6121 ctcgcgccag cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt
     6181 ggttgtaacg ggtgatggtg tagaagttcg gcagaccgta ccagtactgg tagccggtgc
     6241 caacatccag acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta
     6301 aacctgcggc ggcaagctgc gaaatgctgt acttagtttt gaagccattt ggcaagcctg
     6361 gagcctgacc gtttgccatt accgcgacct gatcgccttt cacccagccg tgcgctttga
     6421 aatagttcgc cacgctaccg atcgcatcaa ccggatccca caggttgata tgcccgtcgc
     6481 cgctgaaatc taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg
     6541 ccccggcaaa ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcaaca
     6601 ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg tgggtagtta aatgacagcg
     6661 ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc
     6721 cgataatccc gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg
     6781 catcttcata ctgattccag aaaaccacac cgttctgcac gttgtccggc gtaataaatt
     6841 ttttgcgata acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg
     6901 gtgcctggtt atccatcagc cgcagtaccg aatccagacg tttcgcctgg gagagaattt
     6961 cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg
     7021 cattcgggtt attagcgaaa tcgccgccca tctgcatcac attgtgctgc ggctcaagca
     7081 ggaagccgcc agacggcgtt ccggtggtcg tatcagtctc agtaggtttt ggcttgctgc
     7141 tacaggcggc aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga
     7201 ggggtccatt taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa
     7261 ggaagcggta gtcactgccc gatacggact ttacataact caactcattc ccctcgctat
     7321 ccttttattc aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac
     7381 ttttaaatct ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct
     7441 gaaggagaga acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt
     7501 ccaaaagggc ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag
     7561 cctgctggtt atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt
     7621 tgctcaacgt tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac
     7681 gtttgtcttt tgcaatccga tgaccctaag cctgggtcgc tttatgccgg aaaagtacaa
     7741 acccagctac tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
     7801 tatcaaccct ggcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc atattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg aaagccacgc cgggaaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacagcttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gcggtgctgt ttcaatctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctggcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcctgcgact tcatcccggt cggtttgtcg ctggcggaag ctcgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgaactcc atcctggttt gcaatttacg ctcgggcagc atcgctatcc
     9181 ggtgaccgcc gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccaccagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggta gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcc gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg tcggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgccctgg acgatcagca gcgcatcgtc
    10441 gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggtatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggtgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gcagttagtt ttattcatga tggcgactca ataattcttg atgctggcag taccgttttg
    10981 cagatggtcc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagtctgcat
    11041 atcgtcaatg cgctatctga actggataac gaacaaacca tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgaca aattattcat gggtactgac ggcatcgatc tcaatgcggg cgtaaccacc
    11221 ttcaacgagg tctataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgatttta
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc tgattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacgcggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcaccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcaaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgccgtg atgacgctat
    12061 cccgcaggta gcgttagccg ccagcgtgat ggatgcgatg ctggagctca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg ttggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcgca aagccgtgcc atcgacgcca aagagatcct
    12301 gatgaagcgc aaaatcactg ccgcaccggt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacttg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcgcagg cagcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat ggtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcagattt tgcttaacaa cgggcaccgc tgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgctgcgag tggctctcga
    12661 cagtactacc gcccgatgaa tagcatgttc cagttcgcgc acgttccccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc ccagccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aagtcagcgc gaaatcgccc
    12901 tgccagtacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactgcgg tcatcgccaa cgcgctgaat atcgccatac tgcaacactc gcagcaactt
    13021 ggcctgcaaa gccaacgaca actcgccgat ctcatcgaga aacagtgtgc cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgcccctt tcacatgccc
    13141 gaacagttca ctttccgcca cactttccgg cagtgcggca cagttgagat agatcagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgattaaga cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 caattgcgtc atgcctggcg ataagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgcggcatcg cccggcagca tattctggct ttccagttgc tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cagcaatcag ccgtaactct tcatcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcaa tgcgccaatc aggttttgcc cggcaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcaggaaa gcgcaccacg tccccggcgc gggcaatcgc
    13681 ttcaagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcatactt ttatttatat agtaaaaaca atcagataaa aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gttggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagtt acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ctggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aactgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgact ggtttacgct tccgtaacaa acgcgccagt
    15121 gctttcggct ctcacggctg gagcggcggt gcggtggatc gtctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gcttagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gtcgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtggttaaa gaagaaacct ctgccaccac gacggctgac
    15361 ctcggcccac ggatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag ttccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gaagaactgg catcggaggc aaaatgagta
    15541 acggcattgt gatcattggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatggat gagtacaaca
    15661 aacctgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccgag cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccgt gtggtgaaaa gccagaataa tcagtggcaa tacgacaagc
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgccagt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggtggt ggcttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cggaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgttgttga
    16141 aatctcagtt gcaggggctg gaaaaaacag attctggcat tctggcaacg ctggaccgcc
    16201 agcgcagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaaccgccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctcca gccgattcaa cttagcgcga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccact gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt ggattgttga aaacgttgcc gatgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccaac ctacggggag cgcatgtgtt
    16741 ggtcacatac tgcgttcatg tcgcatccac ctactgtgcc caaaatgtcg gatgcgacgc
    16801 tggcgcgtct tatccgacct acggggacgc atgtgtaggc cggataaggc gtttacgccg
    16861 catccggcaa tggtgtccaa atgcaacacg ttttatccgt tctggacttc acccgctaac
    16921 caacgcgccg cagcaataac cccctgcccc agagacaaac cgccatcacc cgccggtaaa
    16981 ctctgtggaa agagcaatgt gaaatcagcg agataatgcg ccagacgtgc acgcagcaaa
    17041 cggttatgaa taaccccgcc gctaaatacc agcgtagtga taccacgcat cgtggcctgc
    17101 tcacgcatca acgcggcaaa accctgcgcc agcgcatcat gaaacgccca cgcgcgttga
    17161 ttaaccggtg cctgccagtt cagccactgc tgccagaaag tggcgagatc cagttgattg
    17221 tccaccagcg gcattgtcac cggatgcgtc actccgtggc acgaagctgc gagtgcctcc
    17281 agagcacaag ccgcttcacc ttcataactt aacgtggctg gcgcacagcc cagtgccgcc
    17341 gccacggcat cgaacaaccg cccacacgat gacgccagcg gcgcgttaat tccacgctca
    17401 atggcccgcg ccagcacgct ccagttttgc tgttgcacac ttgctgtttc agaataattc
    17461 tgccactccg gcacaaagcg caggcactgc gccagcaggt ttcgccacgg ctgcgtcgcc
    17521 gccaaatcgc cacccggaag cgccactgca ggcaagccgc ccaggtgctc acattcgcga
    17581 tagttcaccc gcagacactc gccgccccac aaagcgccgt tctcccccat accgatacca
    17641 tcgagcgtca aagcaatgac atcaccgcca tccagcggcc actgatgctc tgccagacac
    17701 gccgctgcgt gggcatgatg atgcagcacc gtttgcgtcg gcatattcat ttcgctcgcc
    17761 cactggctgg agacatagcc cggatgcaca tcatgcacaa cgtattgcgg agtgaaatcg
    17821 tagatgtttt gcatcaggcg taacgcttcg cgccactgca tctggatgcc atcgtcactt
    17881 aaatcgccca gatgctgact caacaccgct tgttcaccgc gcaccaggca gaaggtgttt
    17941 ttcagatccg cgccaagaca cagcacaggc ggaacatttt taaagcccgg aggcaaaacc
    18001 agcgcatccg gcacataccc gcgtgaacgg cgcagcattt cgccgctttc gcgcaccacc
    18061 gaatcatcca tccgctgcac gatgtcgcgg ttatgtatca agaatccgtc ggcaatgccc
    18121 tgcaaatccg ccagcgcctg ttcgttgctg atagctggtg gtttaccgct caggttgccg
    18181 gaggtcatca ccagcgggca ttgcagttcc tgtaacagca aatgctggag cgggttcgca
    18241 ggcaacatta ccccgacttc gttaaggtca ggggcgatat catcacaaag ctcaggaacg
    18301 tattttttat ccaccagcac aatcggcgcg gcgggcgtgg taagcaactg gcgcgcagcg
    18361 tctggtaaac cgtcagccac tggcaacatg accgccagcg gtttcgccgg gcgatgtttg
    18421 cgcgcccgca gtgtcgccac cgcgttactg ttacgtgcat cgcaggcaag atgaaatccg
    18481 ccaatccctt tgatggcgac aatgttgccc atttttaact gtgcgatagc cgcctgtaat
    18541 gccgcctctt gttctgcatg ttcaccatga cttacccatt caagatgtgg gccacactcc
    18601 gggcaggcca ccggctgggc gtggaagcga cgatcgagcg ggtcgcggta ctctttgtca
    18661 caagccggac acaacggaaa cgccgccatc acggtaaacg ggcggtcgta aggcatggcg
    18721 cgaataatgg tgaaacgcgg gccgcagtgg gtacagttga taaacggata acgataacgc
    18781 cgttcgcctg gggtattcat ttcggcaaga caggcagggc aagtagcggc atcgggaaca
    18841 atttgcgtat tcatggtgcc gcctgtgctc tggcgtatag tgaactcggt gggcagttgt
    18901 gaccagatgt acggctcacg ctcgacgcta tcaatacgcg ccagcggcgg gcagtgctga
    18961 tgcaattgaa caagaaacgt ttccgggtct tccagcagcc ggacttctac gccatcgccg
    19021 tcattacaga catcgccgtg aagatttaat tgctgtgcca gctgccagac aaacggacga
    19081 aaaccgacgc cctgcacttt gcctcgaata cgcagttgga caccgcaaga tgtgtttttt
    19141 gccattgagt tattcccgcc atcatgaatt gcgtaacccg ccctgccgga cacgactgcg
    19201 tcgtatccgg cagtctcagg tcggcgatac cgccagctcc gtattctacg aatatttccg
    19261 ggaattcctt tgatgccaga acagttctat aagattttta gaacatcagc gccgtacggc
    19321 gacgtttttc tgcgctcagt tgttcaagtt tattacgatc gacacaaatc agcgcatggg
    19381 tcgggcaagc cgccatacac gccgggccgt cttcgcgatg gttgcacagg tcgcatttat
    19441 tggcttcagc tttgtcagcc cgtacattca gccccgcgcc gctgttgcgg atcaccggac
    19501 gtaccaccac ttccatcgcg ccatacgggc aagccaccac gcaggttttg caaccaatgc
    19561 aacgttcctg catcacatga acaaaccctt tatcacggct gatagcacca ttcgggcaga
    19621 cgttagcgca cggtgcatct tcacactgac ggcagactgt cgccgtggaa atgttcacac
    19681 ctttaatgac atggattcgc ggtaaaaagg tttccggggt cagcgatgca cagtcctgat
    19741 tttcctgatg agaaaccacg cacgccactt cacaggtacg gcaaccaata catttactcg
    19801 cgtcagcaat gatgaaacgg ttcatcaaat tctccagcaa tgacagttaa tgcgccgata
    19861 cattcacaaa tcatgccagt ttttaatttg ctgttattta aggaaattaa tttctgtaat
    19921 gcaggaaaaa agatgtcatc gacactagtg acgatgacat gtgatgacaa tgtttatcgc
    19981 gaaagggcaa tgagtgagtc gcggcggatc agttttccgc tgaaggtttt cggcggtgag
    20041 aaatccccgc catcgagcat aaaaatcagc cgtccaataa tttcctgaat catctctgtt
    20101 accggaattt ttacgctgga gagcgccgga acggtgtagg gggcaattgc gatatcatcg
    20161 aatccgataa ctgacacctg ctctggcacc gctacgccgc gctcgtgcaa cgctttcatc
    20221 gcacctatcg ccatatcgtc gttactggca actaacgcgc taaatttagc cccacgttcg
    20281 agcaacgttt ctaccccttc ggccccgctg gcgggcgtcc atttaccgtt agcgataagt
    20341 ttttcattga gcgcaatacc atgctgcgcc agcgcgtctt tatacccagc aagacgttca
    20401 atgctggtgg gggaatccat cgagccggta aggaaagcaa tctcctgatg tccggcgttt
    20461 atcaactctg ccacggcatt aaagctggtc tgtttatgat cgcaccagac gctatggctg
    20521 ctgtttttgc gcaggcggcg attaagcacc attatcggct gactgtgcgc gtcaatgatg
    20581 tcatcgatct catccacgct taaaaaacgc gggtaaatca taatggcgtc gcagcgcaaa
    20641 tccagcagat actgaatcgc ctggcgctct tcttcggcgc tgtgtttacc atctgccaat
    20701 agcaactgcc gccctttctc ttccgccatt cgcgcggcat gaaagagtaa ttcactaaaa
    20761 taaatgccgt ggtaaagcgt gttggtcact accagcccca gcgtctgagt actcttcgcc
    20821 gacagattgc gcgccagcaa gtttgggcgg taaccgctct cttctaccgc ctgaaacacg
    20881 cgatctttag tctcctggct gacgtagcca ttacctgaaa gcacgcggga aacggtcgct
    20941 tttgaaaccc cggcgcgctt cgccacttcc agcatcgtcg tcatcatttt catcccttta
    21001 cacgcaatca acgcagtgta ctgcaccgtt tgccgattgt ccttgcgcaa tcggcgggaa
    21061 aaatattcag gtgaccggtt tcacaaatat aaaaaatgaa caattaactc tcttgcttat
    21121 ttagtgacaa ctattcatga ttttgtgaaa ccggtttcct aattccgttt cagcatcggc
    21181 atttttccgt tacgtcgact gataacaact acatctaccc tactgataac aggataaaat
    21241 ccgatggcca aaaattatgc ggcgctggca cgctcggtga tagcggcact gggcggcgtt
    21301 gataacatct cggcagtcac gcactgtatg acgcgtttgc gctttgttat caaagatgac
    21361 cagcttatcg acagcccgac gttaaaaacc atccccggcg tgctcggcgt ggtacgtagt
    21421 gacaaccagt gtcaggtgat tatcggcaat accgtttcac aagcctttca ggaagtcgtc
    21481 agcctgctgc cgggagatat gcagcccgca cagcccgtgg gtaaacccaa actcacgctg
    21541 cgtcgcattg gtgcggggat cctcgatgcg ctgatcagca ccatgtcacc gctgatcccg
    21601 gcgattatcg gcggatcgat ggtcaaactg ctggcgatga tcctcgagat gagcggcgtg
    21661 ttgacaaaag gatcgccgac cttaaccatt ctgaatgtga ttggtgacgg tgctttcttc
    21721 ttcctgccgc taatggtcgc ggcttcggcc gccatcaaat ttaaaaccaa tatgtcgctg
    21781 gcgattgcca tcgcaggtgt gctggtacat ccgagcttta ttgaactgat ggcgaaagcg
    21841 gcccagggtg aacatgtcga atttgccctg attccggtca ccgcggtgaa atatacctac
    21901 acggtgatcc cggcgctggt gatgacctgg tgcctgtcat atatcgaacg ctgggtggat
    21961 cgcattacac cggctgtgac caaaaacttc ctcaagccaa tgctgattgt gttgattgcc
    22021 gcaccgctgg caatcctgct gattggcccg attggtatct ggatcggtag cgccatttcg
    22081 gcgctggttt acaccattca tggttatctg ggctggcttt cagtcgccat tatgggcgcg
    22141 ctgtggcctc tgctggtaat gaccgggatg caccgcgtct ttacgccaac catcattcag
    22201 accattgccg aaaccggcaa agaagggatg gtcatgccgt cagagatcgg cgctaacctg
    22261 tcgctgggcg gttcatcact ggcggtggcg tggaaaacga aaaacccgga actgcgccag
    22321 acggcgctgg ctgcggcggc atcagccatt atggcgggga tttccgaacc ggcgttatac
    22381 ggcgtggcga tccgcctgaa acgtccgctt atcgccagtc ttatcagcgg ttttatttgc
    22441 ggcgcggttg ccggtatggc ggggcttgcc agccactcaa tggcagcgcc ggggctattt
    22501 accagcgtgc agttcttcga tccggcgaat ccaatgagca tcgtctgggt attcgcggtc
    22561 atggcgctgg cggtggtgct gtcgtttatc ctcacactgt tgctcggctt tgaggatatt
    22621 cctgttgagg aagcgactgc cgaggcgcga aagcatcaga gcgtacaacc gaccgtcgcc
    22681 aaagaagtaa gtcttaattg aggatgaaaa tgtcagtatt tccagaaggt tttttatggg
    22741 gcggcgcgct tgccgccaac cagtctgaag gtgcgttccg tgaaggtggc aaagggctga
    22801 ccactgtcga tatgatccca cacggcgagc atcgaatggc ggtgaaactg gggctggaaa
    22861 aacgttttca gttgcgcgat gacgagtttt atcccagcca tgaggcgacg gatttttatc
    22921 atcgttataa agaagatatc gccctgatgg cagagatggg attcaaggtt ttccgtacct
    22981 caattgcctg gagccgcctc tttccgcagg gcgataaaat cacgcccaat cagcagggca
    23041 ttgcttttta ccgttctgtc tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca
    23101 cgttgtgcca cttcgatgtg ccgatgcatc tggtcaccga atatggctcc tggcgtaacc
    23161 gcaagctggt ggagtttttc agccgctacg ccagaacctg ctttgaagca tttgatggtc
    23221 tggtgaaata ctggctaacc ttcaatgaaa tcaacattat gttgcatagc ccgttctccg
    23281 gcgcgggtct ggtgtttgaa gaaggtgaaa atcaggatca ggtgaaatat caggccgcgc
    23341 atcaccagct ggttgccagt gcgctagcca ccaaaatcgc ccatgaggtt aacccgcaaa
    23401 atcaggtggg ctgtatgctg gcgggcggta acttctaccc ttacagctgc aaaccggaag
    23461 atgtctgggc ggcgctggag aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc
    23521 gcggcgcgta tccggcttac tctgcccgcg tatttcgcga aaaaggggta accatcaaca
    23581 aagcaccggg cgatgatgaa atcctgaaaa acaccgtcga ttttgtctct ttcagctatt
    23641 acgcctcgcg ctgcgcctcg gcggagatga acgccaacaa cagcagtgcg gcgaacgtgg
    23701 tgaaatcgct gcgtaatccg tatctacagg tgagcgactg gggctgggga attgatccac
    23761 tcggtctgcg tatcaccatg aatatgatgt acgatcgtta tcagaagccg ctgtttctgg
    23821 tggaaaacgg cctcggcgca aaagatgaac ttgctgccaa tggcgagatt aacgatgact
    23881 atcgcatcag ctatttacgc gaacatatcc gcgcaatggg cgaagcgatt gcagatggca
    23941 ttccgctgat gggctacacc acctggggct gtattgattt agtttccgcc tctacgggtg
    24001 aaatgagcaa acgctacggt tttgtctacg tagaccgtga cgacgcaggc aacggcacgc
    24061 tgacgcgcac gcgtaagaaa tcgttctggt ggtataaaaa agtaattgcc agtaatgggg
    24121 aagatttaga gtaagtaacc gtgccggatg cggcgtgaac gccttatccg gcctacatgt
    24181 cgcacgaacc atgaaggccc gataagcgca gcgcatcggg caatctgggg tttggcatca
    24241 gtcaaaatca aagaaaccat tccggcttaa cgccaaatcg ggctgaaagt gccttaatgt
    24301 gggatatcgt cagggatcgt tgtccagaca aaatctgact gaccagggat ttagagccga
    24361 tttcgttctt caaatccgca taggtcagat tatgttgatc aatcaacgtg cgaagtaacg
    24421 ccacgccaac cggcattgcc gcaacggctt tgtcgaactc tgcaaacttt tcgttgttgt
    24481 tttcatactc tgcaatgcgg ctcgccagaa agtcgataag tggattggtg tcatcttctt
    24541 caatcaaata ttcgaccagt tccagtgctt cacgataatc atcttctgag tcactgcctc
    24601 ccagaaaggg gactgcattg accagttctc ttgttgcttt aacagcgcgt gcagcattag
    24661 cagtcattct ttgttctctc tgtagtaacg tgacgtctgt ctgcacgtaa tccccctcat
    24721 agccactctg caaaaagtca gttctggttc accccataaa gcacatcccc ctttatcaag
    24781 caacgctgcg tctgcgccag atcttccgtt tgcgggtcgc gcccggcgat gggtttgagg
    24841 acgagtttac ggttgggggt ataagtaaaa cgcgtgagcg cgggatagct taacttgcca
    24901 aaccagccgt gaacacgctg ccatttggcg tcttttaccg atacagactg gttatccagt
    24961 aaccggaatc aaactgcata tccagcaatt gcgtgtggcc attcctttgt aaatccagca
    25021 aatcatcaat gccagtttca cttgcggtat aaaaaccatc ggactgaaaa acacacgggc
    25081 gaccttgctg attaaaggtc atcagaatca aaaacgcgtt aggtgccaga ccatttcctg
    25141 aaataccccg ccagataacc agatcctgaa tgccgttacg atcaagatcg gcacggtaaa
    25201 gttgcgcatt acccgccata ttttgcagca ctcccacagg cacagaccag gatttaccgg
    25261 cgcgatcttt accgttcaat tgccattcac tatcatcatt tggcatcttt tcaagcgtca
    25321 cgccagcaac gccgactttt ctaaaatgcc agttttgctc ggcatcataa ggcatggtca
    25381 ggagaggttt cagcgcccgc ttttgcgcgt attcgctgtc actcattggc agatattcgg
    25441 taaagctggt ggcctgtgca ggcagcacta agctcaataa aaaagcgcac atccctgttc
    25501 tgatgttcaa ttccctgaat tccctgatgg agaattattc ttcttccacc gctaactgcg
    25561 caaatccacc atttccttcc cagccttcca gtcgttgata aacggtttct accgcatctt
    25621 taatcggctg ggtcatcggg tagtaaaagc cgacgatatc cggctgaatg ccgaggaaaa
    25681 tcacttcacc aatgtcttct tttaactggt cgataaggta attcaacggc atgttatggg
    25741 tagtcatcat aaacatctcg gcgatatcat ccgggtcgat gatgcggatt tcgccggggt
    25801 ttagccccat atccgtggcg tcgacaatca gcagtcgtgt cgggcgcagt tcacggatgg
    25861 cgacgatgtc gttttccggt gcactaccgc cgtcaatcac cacccagtta cctttcggcg
    25921 cggcggcgca cttttccgcc agcagcgggc cagcgccatc atcgcccatc atgctattgc
    25981 caacacagag taaaacgtca gtcacgcagt ctcctcacca tcaaatagat ggcgttttcc
    26041 tgatgaatat catgcagcat acttaacagc attgtactcc attccttctg ctgcgaagtt
    26101 tgcgctgccc gggctttatc gaatgcgttg gccagcatcg gcacatggtt aatgtcgatg
    26161 acgatctcgc catatttcgg tacgccttcc attttgcggc gggcttcgct gcccgcctcc
    26221 agagtggcaa tccatgccag atattcatcc catgggcagg tgagcgccgc ttccaggcaa
    26281 tcgataaccc caaggtggtg accaatcgcc aggctgtaat agaccacctg ctgcgcctcg
    26341 gcgggcgtgg catcgttctc atcaataaat ttacggctca gttgactgaa caccaccttt
    26401 tcactcatcg gatacgcgcc tcttcaacaa catgattcag atggctgaca atctcgttca
    26461 gacgcggatc gttttccgct tccagccagc gtgcaacctg ttcttcgccc tgccctaact
    26521 gtgtcaggaa gtcatcggca atctgacgac cgtaacgata acctgccagg cggcgtgctt
    26581 cgcgatccac tttcacacgc agcggctgca ccatatcgcc atgcaggatt tccgccggtt
    26641 gttcatcctg ctcacccggg ccacgggcgt gaattttctg ctccagcagg ccgagcgcca
    26701 ttgcaaagcc gtacagcgtg gcggcaggcg ttggcgggca accagggatg tagacatcta
    26761 ccgggacgat tttatccgta ccgccccaca cgcagtagag atcgtggaag atcccgccac
    26821 tgttaccgca ggcaccgtag gagatacaga ttttcgggtc cggcgcggac tgccacgcac
    26881 gcagcgcagg ggatcgcatt gcacgggtga ccgcgccggt aaacagtaaa atatccgcat
    26941 gacgcggtga aggaacgact ttaatgccga agcgttctgc atcaaacagc ggcgaaagcg
    27001 tggcgaaaat ttcgatttcg cagccgttgc agccgccgca gtccacgcgg taaacgtagg
    27061 cagaacgttt gatttttttc agtaacgacg ccttcatgct ggcgatggat tcatccaccg
    27121 tcatcgggac cggaatgccg ttggcgtcac gggggcctaa taaattgctc atcagatggc
    27181 ctctttcata tggcgagtca gttcaatacg gtcggacggc accaggcatt tctggcgctt
    27241 acattccggg caagtctcaa agctttcgcg gtggttttcc gcgcggctgt cgccgttgtg
    27301 cttaagtagc gcaatggcgt agtcgatctc tttctggaca gcgaaaggac gattgcagac
    27361 gcgacagttg cacagcgcga agcgggactg ttgcaggaag tcttctttct tccacaccgc
    27421 cagttcgtac tcttgcgaca gtttgatcgc cgccgtcggg caaacttctt cgcagcgtcc
    27481 acagaagatg cagcgtccaa gattgaactc ccacgcaagc tctcctgtgg cgaggtcggt
    27541 ttcaaccgtt aaggcgtttg acgggcaggc attgacgcag gccgcgcagc cgatgcactg
    27601 ctgcgggttc tgctctggct taccacggaa gtttttatca accgcaatcg gctccagcgg
    27661 ataagacgag gtcgccgtgc cggttttgat aacttttttg ataaaggtaa acatggcgat
    27721 tccttatttc agcggcgagt ttttacgctc aatgctgtag cgctcgagtt ctttgtacgg
    27781 caccactttg ctcttcttct tacgtacatc gaccaccgtc atgcggtcgg tacaggagta
    27841 gcaagggtcc aggctaccga taatcagcgg cgcatcggaa acggtattgc cacgcagcat
    27901 gtagcgcagg gtcggccagt tcgcgtaggt cgcggcacgg cagcgccagc ggtacagctt
    27961 ctggttgtcg ccggtcatgc tccagtggat atcgtcgccg cgcggcgctt cggcaaagcc
    28021 cagcgcgaaa cggtgcggaa tgtaggtaaa gccttccacc atcagcggac cgcccggcag
    28081 gttatccaga ccgtagtcga tcatgttcag cgcggtataa acttcgttga tacgcacttt
    28141 cagacgggaa ataacgtcgc agccctgctc gctgtggact tccattggca gcaggccata
    28201 accgacaaac gggtgatcgg cgcgagtatc gcgggcgtga ccgctggcgc gaaccatcgg
    28261 gccaacgtta ctgaaatcgc gggcgatttc cgggtccaga cgaccaatgc cgacggtgcg
    28321 ctgttccatg ttcggcgtac tcagcagcac atccaccagc tcctgcactt cacgacgcat
    28381 ctgttgcgcc agctggcggg tctggatcat gtcatctttc aacagatcgc gacgaatccc
    28441 gccgatcagg ttcaggccgt aggttttacg cgcaccggtg aggatctctg ccattttcat
    28501 ggaggtttca cgcacgcgga agaactgcat aaagccggag tcaaagccgg taaagtggca
    28561 ggcgagaccg aggttgagca gatgcgagtg caggcgttcc acctccagca gaatggcgcg
    28621 gatcatctgc gcacgttctg gcacctgaat acccatcgcg ttttctaccg acgtggtgta
    28681 ggcagtgctg tgggcaaagc cgcagatccc gcacacacgg tcagagagga aggtcacttc
    28741 gttgtaaccc atacgggttt ccgccagttt ttccatgccg cgatgaacgt agaacagacg
    28801 gtagtcggcg tcgataatgt tttcgccatc gacaaacaga cggaagtggc ccggttcgtc
    28861 agaagtgacg tgcagcggac caatcggcac gacgttgttt ttcttgtcgc ccagttcgtt
    28921 gatgaactcg taggtttcag catcggtagt cggtgccgga cgctgacgat aatccatgct
    28981 gtctttacgc agcggataaa gttcatccgg ccagtcatcc ggcagcacca gacgacgttc
    29041 atccggcaga ccaaccggaa tcaaaccgta catatcgcgc acttcgcgct cgccccacac
    29101 cgccgccgga acgcgcggcg tcacggacgg atactccggt ttgttggcgt caacttcgac
    29161 gcgaaccgtt acccaacact tggtgccctt ctccatcgac agcacgtaat aaacggcgta
    29221 atgaccattc agtttgcgtt cgtcgttacc aaacagcacc gacagccagc caccctgctt
    29281 gtagtagaga aactccacca cttccggcag gtagttcacc ttcacggtca cggtcagctg
    29341 atctttggtc tgccaggcgt ggtccagcac gacgcccgga aatgcctcat tcagcgcggc
    29401 gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc
    29461 agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat gcgcggagta
    29521 atatcaagac gcagacgggc catgctgttt tcgaacagcg cgataaccag gacgccgacg
    29581 accagtttga cgatggcaat caccagcgcc agcagcagcc caccggcggt gaaggtttcc
    29641 atctgtcccc acgggataaa tacgccgacg aacatctgca gcaccaccag ctgtttcagg
    29701 ctgatacccc atttcatgac gccaaagccg ctgccgctgt attcagagag cgggccttcc
    29761 tgcagctcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaggtg
    29821 gcgaacgcac aggcacaaag cgccagtacc agcgggatgc tctggctcag cggccagtga
    29881 taaacggtgt cggtgatgtt gctgatgttg gtggaaccgg caacctgtgc ggcaacccac
    29941 agaccaagca gcagcatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcgccg
    30001 atagcggtaa acgggctacc ggtatccaga ccagaaatgg caaagaagaa acgcgcgatg
    30061 gcaaagagat acagtaaggt gattaaatca cccagttgcg gcagcggaga accgacggtc
    30121 accaccggca gcgcagtagc gatagtcagc atgacgccca ccatcacata cggcgtcagg
    30181 cggaacaccc agccggaggc atccgggcca acgctctggc ggcccagcag tttgataatg
    30241 tcacgatact cctgcaacac acccggccca cgacggttat gtaagcgggc tcgcgccacg
    30301 cgggtaatac cggagagcag cggcgcaacg gcaaatagca ccagcgcctg aattaacgga
    30361 tataaaacac tcattctcag gctcctcgtg aaacaataat caccaccagc accgccagtt
    30421 caaccagcgc catccggcgg aacagcaacg cactcccctc gcactgccag cccggcacca
    30481 gagacaccgg attcagccat ttgcgtagtt tcagcaccgg cgcaaacgcc tgtttcaccg
    30541 gcatggcaaa accgtgagcg gtaatcacca ttgatttttc gtggtcgtag ccgcacaccc
    30601 aggccgcacc gcgggaacgc gacggcaaac gatcgccttt gcaaatcgcc ataatgatga
    30661 atggcagcag cgggcaggca atcagcagca acgtgatcat cggttgagaa acggtggtgt
    30721 tagcaggctc cagcggcaga ggtacagcag cagagagcat cggcagcagc cacggcgcag
    30781 caacaccgcc aattacgcag caaatcgcca gtgccactac gcttacgctc atcaggagcg
    30841 gcgcacaggt ggcgttttcg gcttctttgg tacgcggcgc gccgaggaac gtgacgccat
    30901 agactttcgc catacacatc accgccagcg caccggtaat tgccagaccc acagcgagca
    30961 gcggccccag cagacgggca acaaacgcgc cactattgct cagtttgaaa aaggattgat
    31021 agataaccca ttccccggca aacccgttca gcggcggcag cgcagccatt gccatcaacc
    31081 cgactaacat ggcgatggag ataaccggca ttttcttgcc aataccaccg agtttttcga
    31141 tatcgcgatg accggtacgg aaccagacgc tccccgcccc gaggaacagt acgcttttga
    31201 acaggctatg gttaagcaga tggtacagac caccgaccag accaagggca atcagcgccg
    31261 gttgttcgag cgcgataccc gttacgccag cgcccagccc cagcaggata atgccgatat
    31321 tttccagggt gtggtaagcc agcaggcgct ggatgttgtg ttccatcaac gcatacagac
    31381 caccgacgaa cgcggtgatc atgccgagca ccagcagcgc gatcccccac cacagcggcg
    31441 cgttaccacc cagcagcgac agggttaaaa tgcccagcag gccaattttc atgactaccg
    31501 tagaaaacaa cgcggcagct ggcgcagagg cgttagcatg tgcctgcggc acccagccgt
    31561 gcagcggaat aatcccggcc agcaggccaa agccaatcac accgagcagc cagatatcgg
    31621 aaccgagcgg cagctgttgc atacgcatat ccagcaggcg cagatccagc gtgccataac
    31681 gctgccacag cagccagcaa gcaatcgcca gcagcagagt gccaagacgc cccagcgcaa
    31741 accacagttt gccctctttg ctgttgctgg tgaggaacac cgcacacaag gccatgactt
    31801 cggccattac cacgaacatg ccgaggttgc tggcaatgac ggcgcagacg gcggcagcca
    31861 tcaacatatt gatctgcaag ccgttgcact tcacctgcgc gtggcgatgc cagtcaatgt
    31921 tatagaggct gacaaacaga ccgcaaaggc cgagggtaat cagccaaatc gcgttaagcg
    31981 gggagatttg cacatcgtag cttaccagcg acagcgcacc gctcacgcca accgcgccag
    32041 tcagcacagt gaagcccgcg gctgccgtat acaggctacc aaccgcgccg ccaattccgg
    32101 ctatccagcc acttagcgtc ttttgaaaag aaaagagaaa tgccagaaca gcggcggcaa
    32161 taaaccacgc cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg
    32221 agcctgctga aacaaggtga gatcgccaaa gtccgtatta aaagtcagct cacgcttacg
    32281 tttgctgacg cgggcgatat cggtgttatc caccagatgc agggctttgg tcgggcacat
    32341 ccgcacgcag gccggacctt gttcatcaaa actgcaaagg tcacatttga cggcgatcgc
    32401 gcgaataccc ggcacccagt caagcagcgt actgatacgc gccggagcag gcggtgccgg
    32461 tggcgctttc ggggtattgg catttgcagg aatatccagc ggacggctgc cggaaaattc
    32521 aattgcgcca aacgggcagg cgatgccgca cagcttgcag cttacgcaca ggctttcatt
    32581 caactgcacg gccccatcaa cgcgggtgat ggcgttaacc gggcagacca ccgcgcaggg
    32641 tgcatcttca cagtggtgac agagctgcgg cgcagattct ttttcattta gcatcactct
    32701 caggcgcggc attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca
    32761 agtgtggcag ccgatacaga gcgtggagtc agcaattaca aaacgattca ccaggcattc
    32821 ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat
    32881 cgacacgccc atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg
    32941 ctgccgggct ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatgg
    33001 cggtgtataa gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt
    33061 cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg
    33121 caatccgctg tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt
    33181 taaagccatc cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt
    33241 ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct
    33301 gaaccagcga attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga
    33361 tgtaatcggc tttctcgctt atttcccaaa tagtcattgt caggttaccc gtttaacaga
    33421 gatgccagct ttaagcattt tttgtgccaa cttttaattt attgttatta aagagatttt
    33481 taagctaaag atgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat
    33541 tcaccgcagg gattaatcaa cactggcaca attattgctt gtagctggca atagttaatg
    33601 ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat cgaacagcag
    33661 gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg cgcattttct
    33721 tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg cagcgtggcg
    33781 gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga aacatgccaa
    33841 cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca tggtgacatg
    33901 ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga ccaggagtga
    33961 gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt atatcgaggg tgatgaacat
    34021 aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc gaagatgaaa
    34081 atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg cgacctgcat
    34141 tacggtcatg gcgaagcggg cactcacgca ccgggcatga gccagcgtcg gatgctggaa
    34201 gtcgaaattg acgtgctgga caaaaacaac cgtctggctg aacgcaaccg cgcgcgcttt
    34261 gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg taaaaccacc
    34321 ctgctgacgg aaaccttaat gcgcctgaaa gacagcgttc cgtgcgcggt tattgaaggt
    34381 gaccagcaaa ccgtgaacga tgccgcacgc attcgcgcta ccggcacacc agcgattcag
    34441 gtgaacaccg gtaaaggctg ccatcttgac gcacagatga ttgccgacgc cgcaccgcgt
    34501 ctgccactgg acgataacgg tattctgttt atcgaaaacg ttggcaacct cgtatgcccg
    34561 gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac cgaaggtgaa
    34621 gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct gctcaacaaa
    34681 gttgacctgt tgccgtatct caactttgac gttgaaaagt gcatcgcctg cgcccgcgaa
    34741 gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg gatggaccag
    34801 tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag atccgcacca
    34861 ttgacggcaa ccaggcgaaa gtcgacgtct gcggcattca gcgcgatgtc gatttaacgt
    34921 tagtcggcag ctgcgatgaa aacggtcagc cgcgcgtggg ccagtgggta ctggtacacg
    34981 ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc gacgccttac
    35041 aaaacatgtt tgacgttgag ccggatgtcg gcgcgctgtt gtatggcgag gaaaaataat
    35101 gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg agcatctgcg
    35161 cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta tggaagtgtg
    35221 tggcggtcat acccacgcta tctttaaatt cggcctcgac cagttactgc cggaaaacgt
    35281 tgagtttatc cacggtccgg ggtgcccggt gtgcgtactg ccgatgggta gaatcgacac
    35341 ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg acgccatgcg
    35401 cgtaccaggg aaacagggtt cgctgttgca ggcaaaagca cgcggtgccg atgtgcgcat
    35461 cgtttactcg ccgatggatg cgttgaaact ggcgcaggag aatccaaccc gcaaagtggt
    35521 gttcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcactc tgcaacaggc
    35581 gaaagcgcgt gatgtgcaga atttttactt cttctgccag catattacgc ttatcccgac
    35641 gcttcgcagt ttgctggaac agccggataa cggtatcgac gcgttcctcg cgccgggcca
    35701 cgttagtatg gttatcggca ctgatgccta taattttatc gccagcgatt ttcatcgtcc
    35761 gctggtggtg gctggtttcg aaccgcttga tctactgcaa ggcgtggtca tgctggtgga
    35821 gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg taccggatgc
    35881 cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc tgtgtcaacg gcgacagcga
    35941 atggcgcggc ttaggcgtga ttgaatcttc tggtgtacac ctgacgccag attatcaacg
    36001 attcgatgcc gaagcgcatt tccgcccggc accgcagcag gtctgcgatg acccgcgcgc
    36061 acgttgtggt gaagtattaa cgggcaaatg taagccgcat caatgcccgc tgtttggtaa
    36121 cacctgtaat cctcaaaccg catttggtgc actgatggtc tcctccgaag gagcgtgcgc
    36181 cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca actcgcccac
    36241 ggcagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga agcctttgcc
    36301 aacccgtggc tggcagaaca ggaagatcag gcacgtcttg agctggcgca gctggtagcg
    36361 gaaggcgacc gtctggcgtt ctccaccgac agttacgtca ttgacccgct gttctttcct
    36421 ggcggtaata tcggcaagct ggcgatttgc ggcactgcga atgacgttgc ggtcagtggc
    36481 gctattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc gatggagaca
    36541 ctgaaagccg tagtgaccag catggcagaa accgcccgca cggcaggcat tgccatcgtt
    36601 actggcgata ctaaagtggt gcagcgcggc gcggcagata aactgtttat caacaccgct
    36661 ggcatgggcg caattccggc gaatattcac tggggcgcac agacgctaac cgcaggcgat
    36721 gtattactgg ttagcggcac gctcggcgac cacggggcga ctatccttaa cctgcgtgag
    36781 cagctggggc tggatggcga actggtcagc gactgcgcgg tactgacgcc gcttattcag
    36841 acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg tggtgtgaac
    36901 gcggtagttc atgagttcgc ggcagcctgc ggttgtggta ttgaaatttc agaagcggca
    36961 ctgccggtta aacctgccgt gcgcggcgtt tgcgaactgc tgggactgga cgcccttaac
    37021 tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcggcaga gcaagtgctg
    37081 gcagcgttac attcccatcc actgggaaaa gacgcggcgc tgattggtga agtggtggaa
    37141 cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac gaaccctcga tttaccacac
    37201 gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt tagtcaatga
    37261 ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata caccgatgag
    37321 tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc agcccgatct
    37381 ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc
    37441 ggctattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat cgcgtgaaaa
    37501 agacaccccc attaaatatg aagacgaaac tgttctggca cacggtccgg tacgccgcat
    37561 tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa cctggccgca
    37621 gctggtcgca ggtgggctat accccaaatt tggtcactat tgcctgatgc cactggcggc
    37681 ggaagggcat atttttggtg gctgtgaatt tattcgttat gacgatcgtc cctggagcga
    37741 aaaagagttc aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca ccgaacaaat
    37801 ccagagccgc gtcgtcaaca atgtcgacta tgagttgtta tgccgggagc gcgataactt
    37861 ccgcatcctg gttgccatta ccaacgcggt gctttcccgc ctggatatgg acgaactggt
    37921 cagcgaagtc gccaaagaaa tccattacta tttcgacatt gacgatatca gcattgtctt
    37981 acgcagccac cgtaaaaaca aactcaacat ctactccact cactatcttg ataaacaaca
    38041 tcccgcccac gaacagagcg aagtcgatga agccggaacc ctcaccgaac gcgtgttcaa
    38101 aagtaaagag atgctgctga tcaatctcca cgagcgggac gatttagccc cctatgaacg
    38161 catgttgttc gacacctggg gcaaccagat tcaaaccttg tgcctgttac cgctgatgtc
    38221 tggcgacacc atgctgggcg tgctgaaact ggcgcaatgc gaagagaaag tgtttaccac
    38281 taccaatctg aatttgctgc gccagattgc cgaacgtgtg gcaatcgctg tcgataacgc
    38341 cctcgcctat caggaaatcc atcgtctgaa agaacggctg gttgatgaaa acctcgccct
    38401 gaccgagcag ctcaacaatg ttgatagtga atttggcgag attattggcc gcagcgaagc
    38461 catgtacagc gtgcttaaac aagttgaaat ggtggcgcaa agtgacagta ccgtgctgat
    38521 cctcggtgaa actggcacgg gtaaagagct gattgcccgc gcgatccata atctcagtgg
    38581 gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg gattgctgga
    38641 aagcgatctg tttggtcatg agcgtggggc ttttaccggt gccagcgccc agcgcattgg
    38701 tcgttttgaa ctggcggata aaagctctct gttcctcgac gaagtgggcg atatgccact
    38761 ggagttacag ccgaagttgc tgcgtgtatt gcaggaacag gagtttgaac gtctcggcag
    38821 caacaaaatc attcagacgg acgtacgttt aattgccgcg actaaccgcg atctgaaaaa
    38881 aatggtcgcc gaccgtgagt tccgtagcga tctctattac cgcctgaacg tattcccgat
    38941 tcacctgccg ccactacgcg agcgtccgga agatattccg ctgctggcga aagcctttac
    39001 cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc attcctgccg agacgctgcg
    39061 caccttgagc aacatggagt ggccgggtaa cgtacgcgaa ctggaaaacg tcattgagcg
    39121 cgcagtattg ctaacacgcg gcaacgtgct gcagctgtca ttgccagata ttgctttacc
    39181 ggagcctgaa acgccgcctg ccgcaacggt tgtcgcccag gagggcgaag atgaatatca
    39241 gttgattgtg cgcgtgctga aagaaaccaa cggcgtggtt gccgggccta aaggcgctgc
    39301 gcaacgtctg gggctgaaac gcacgaccct gctgtcacgg atgaagcggc ttggaattga
    39361 taaatcggca ttgatttaac tgcaaattgc cggacagatc tgcctgtccg gcatactatt
    39421 cacgagtttt tttcggacga tatttttccg gcagttctgg caccggacgc ttgtcatcga
    39481 tgagatgacg cacggttaag atcggatgac gccacagcat tcgcggcccg gcccaacgca
    39541 taatctgttt catctcttca cgctttgctg gctggtaaca gtgcaccgga cactgcttac
    39601 aggctggttt ctcttcgccg aacacacatt tatccagccg cttttgcgcg taaacaaaca
    39661 acgcctcgta atgctccggc tccgctgacg cctgcgggca tttcgcttga taaagatcga
    39721 tcattttttt aatcgtcagt ttttcacgag agatacgctt gccggacatg ctgcctccac
    39781 ctcattaaga tgtatttata ttacatctta atcttaaagg gcactatgac tctaaagaag
    39841 aaaggttcgc caaccgacgc aattttgcgt acttgcttca taagcatcac gcaaaagctg
    39901 caaaacagca tctttcccgg aaccagcatc aagaactcac cgttcgcttc ttcccctgaa
    39961 atgattaact ccggtatcat gtgcgcctta tgtgattaca acgaaaataa aaaccatcac
    40021 accccattta atatcaggga accggacata accccatgag tgcaatagaa aatttcgacg
    40081 cccatacgcc catgatgcag cagtatctca ggctgaaagc ccagcatccc gagatcctgc
    40141 tgttttaccg gatgggtgat ttttatgaac tgttttatga cgacgcaaaa cgcgcgtcgc
    40201 aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg atcccgatgg
    40261 cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat cagggagagt
    40321 ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg gttgagcgca
    40381 aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta caggagcgtc
    40441 aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac gcgacgctgg
    40501 atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg atggcggcag
    40561 agctgcaacg cactaatccg gcggaactgc tgtatgcaga agatttcgcc gagatgtcgc
    40621 tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg ggagtttgaa atcgacaccg
    40681 cgcgccagca gttgaatctg caatttggca ctcgcgatct ggtcggtttt ggcgtcgaga
    40741 acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa gatacccaac
    40801 gtacgactct gccgcatatt cgttccatca ccatggaacg tgagcaggac agcatcatta
    40861 tggatgccgc gacgcgtcgt aatctggaaa tcacccagaa cctggcgggt ggtgcggaaa
    40921 atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggcagccgt atgctgaaac
    40981 gctggctgca tatgccagtg cgcgataccc gcgtgttgct tgagcgccag caaactatcg
    41041 gcgcattgca ggatttcacc gccgagttgc agccggtact gcgtcaggtc ggcgacctgg
    41101 aacgtattct ggcacgtctg gctttacgaa ctgctcgccc acgcgatctg gcccgtatgc
    41161 gccacgcttt ccagcaactg ccggagctgc gtgcacagtt agaaactgtc gatagtgcac
    41221 cggtacaggc gctacgtgag aagatgggcg agtttgccga gctgcgcgat ctgctggagc
    41281 gagcaatcat cgacacaccg ccagtgctgg tacgcgacgg tggtgttatc gcatcgggct
    41341 ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat tatctggagc
    41401 gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac gctaaaagtt ggctttaatg
    41461 cggtgcacgg ctactacatt caaatcagcc gtgggcaaag ccatctggca cccatcaact
    41521 acatgcgtcg ccagacgctg aaaaacgccg agcgctacat cattccagag ctaaaagagt
    41581 acgaagataa agttctcacc tcaaaaggca aagcactggc tctggaaaaa cagctttatg
    41641 aagaactgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc gcgagcgcgc
    41701 tggcggaact cgacgtgctg gtgaacctgg cggaacgggc ctataccctg aactacacct
    41761 gcccgacctt tattgataaa ccgggcattc gcattaccga aggccgccat ccggtggttg
    41821 aacaggtgct gaatgagccg tttatcgcca acccgctgaa tctgtcgccg cagcgtcgca
    41881 tgttgatcat caccggtccg aacatgggcg gtaaaagtac ctatatgcgc cagaccgcgt
    41941 tgattgcgct aatggcctat atcggcagct acgtaccggc gcaaaaagtc gagattggcc
    42001 cgattgaccg tatctttacc cgcgtaggcg cggcagatga cctggcttcc gggcgttcga
    42061 cctttatggt ggagatgacc gaaaccgcca atattttaca taatgccacc gaatatagtc
    42121 tggtattgat ggatgagatc gggcgtggaa cgtccactta cgatggtctg tcgctggcgt
    42181 gggcgtgcgc ggaaaatctg gcgaataaga ttaaggcatt gacgctgttt gccacccact
    42241 atttcgagct gacacagtta ccggagaaaa tggaaggcgt cgccaacgtg catctcgatg
    42301 cactggagca cggcgacacc attgccttta tgcacagcgt gcaggatggc gcggcgagca
    42361 aaagctacgg cctggcggtt gcagctctgg ccggcgtgcc aaaagaggtt attaagcgcg
    42421 cacggcaaaa gctgcgtgag ctggaaagca tttcgccgaa cgccgccgct acgcaagtag
    42481 atggtacgca aatgtctttg ctgtccgtac cagaagaaac ttcgcctgcg gtcgaggcac
    42541 tggaaaacct ggacccggat tcactgactc cgcgtcaggc gctggagtgg atttatcgct
    42601 tgaagagtct ggtgtaataa aaattcccga tagtcttttg ctatcgggaa tattaacgac
    42661 aactgacgaa tcaaataaaa ataccctgta taataggaaa gcttatttta cagggtaaaa
    42721 ccatgccatc tacacgctat caaaaaatca atgcccatca ctatcgccat atatgggtcg
    42781 ttggtgatat tcatggtgaa tatcagttat tacaatcccg cttacatcaa ctctcttttt
    42841 tccccgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg ccggagagtc
    42901 ttgacgtcct gcgcctgcta aaccagccct ggtttatctc cgttaaaggc aaccacgaag
    42961 caatggcgct ggatgcgttc gcgaccggcg atggcaatat gtggcttgcc agcggtggtg
    43021 actggttttt cgatttaaat gattcagagc aaaaagaagc tacagatctg ttgctgaaat
    43081 tccatcacct tccacatatt attgaaatca ctaacgacaa cataaaatat gtcatcgcac
    43141 atgcagatta tccggggagt gaatatctct ttggtaaaga aatagcggag agcgaattac
    43201 tctggcctgt tgatcgtgtg cagaaatcgc ttaatggcga gttacaacaa ataaacggcg
    43261 ctgattattt tatatttgga catatgatgt ttgataacat tcagacgttc gctaaccaga
    43321 tttatattga taccggatcg ccgaaaagtg ggcggctgtc attttataaa ataaggtagt
    43381 ctcatgcttc ttctgtgaag catgagtaac cagatgttat tgcaggccat tattcatttt
    43441 tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc tccggcaaac
    43501 cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat gtcgccacct
    43561 gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcactg gcaatcgccc
    43621 gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc catgacgatg
    43681 cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga
    43741 cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg caattactgt
    43801 tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac cacagtcagt gactccatgt
    43861 gaatcagatg ctgtgctatc gctaacgtgg tcgttcccgc atccagatag atacaacttc
    43921 ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc attgccagcg
    43981 cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg
    44041 acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga atggtcatat
    44101 gtgacacatt cattctgtcc gttagttcag caatactgac aatgcctttt tcagctacca
    44161 tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc ctttgtcctg
    44221 ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca
    44281 taaaataaca cacaatgtta atttatgtga atcagctcac catatcgcta tcttccagcg
    44341 cttatattca caatatcaaa caaaatatca cttaaattaa caaggagagc agatgaaaac
    44401 gggatctgag tttcatgtcg gtatcgttgg cttagggtca atgggaatgg gagcagcact
    44461 gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca atgcctgcgc
    44521 tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa
    44581 actggacgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg
    44641 tgaaacaggc gttgcacaac atctgaaacc cggtacggca gtaatggttt cttccactat
    44701 cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc tggaaatgct
    44761 ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc
    44821 ctccggtagc gatattgcct ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa
    44881 agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta ttcaccagtt
    44941 gttagcgggc gaacatattg ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg
    45001 gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt
    45061 cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag ccgtcgatat
    45121 ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact tcccgctgcc
    45181 attggcctca acagcattga atatgttcac cagcgccagt aacgcaggtt acgggaaaga
    45241 agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag cgaaatcatg
    45301 atcaagattg gcgttatcgc cgatgatttt accggcgcga cggatatcgc cagttttctg
    45361 gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc caacaggtaa aatgccggaa
    45421 gcaatcgacg cactggtgat cagcctgaaa acgcgctcct gtccggtggt tgaagccaca
    45481 cagcaatcgc tggcggctct gagctggttg caacagcaag gttgcaaaca gatctatttc
    45541 aaatactgct ctactttcga cagtacggcg aaaggtaata tcggcccggt taccgatgca
    45601 ttaatggatg ctctcgacac gccgtttacg gtcttctctc cggccctgcc ggtcaacgga
    45661 cgtacggttt atcaggggta tttgttcgta atgaatcaac tgctggccga atccgggatg
    45721 cgccatcacc cggtaaatcc catgaccgac agctatcttc cccgtctggt tgaagcgcaa
    45781 tccacagggc gctgcggcgt cgtttcggca catgttttcg aacaaggtgt ggatgccgtt
    45841 cgtcaagagc tggctcgctt acagcaagag ggctaccgct acgcggtgct tgatgcgctg
    45901 accgaacacc atctggaaat tcagggagaa gccttgcgcg atgccccact ggtaacgggc
    45961 ggttctggtc tggcgattgg cctggcccgg cagtggacgc aagaaaacgg taaccaggct
    46021 cgcaaagcag ggcatccgct cgctgggcgc ggcgtagtgc tctccggttc atgctctcaa
    46081 atgaccaacc gccaggtagc acattaccgt caaattgcac cagcccgtga agttgatgtg
    46141 gcacgctgcc tctcaactga aactctggct acttatgcac acgaactggc agagtgggtt
    46201 ctgggccagg aaagtgtact tgctccactg gtttttgcca ccgccagcac tgacgcattg
    46261 gcagcaattc aacagcaata cggtgcacaa aaagccagtc aggcagtaga aacactgttt
    46321 tctcaactag cggcgcggtt agcggcggaa ggcgtggcac gctttattgt cgcaggcggt
    46381 gagacctccg gcgtagtcac acagagcctg ggcataaaag ggtttcatat tggcccaacc
    46441 atttcccccg gcgtgccgtg ggtaaacgca ctggataagc ctgtctcact cgcccttaaa
    46501 tctggcaact tcggtgatga agcctttttt tcacgagccc aaagagagtt tttatcatga
    46561 gcgatttcgc aaaagtagag cagtctttgc gagaggagat gacgcggatt gccagttcat
    46621 tctttcagcg cggctatgca accggttcgg ctggcaatct gtcgctgctt ttacctgacg
    46681 ggaatttact ggcgacaccg acaggttcat gcctgggcaa tctcgatccg cagcggcttt
    46741 ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa accctcgaaa gaggtgctct
    46801 ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca
    46861 catggtcgac ggcgctttcc tgcctgcaag ggctggacag cagcaacgtt attcgtccgt
    46921 tcacaccata cgtggtgatg cggatgggaa atgtcccgct ggtgccttat taccgaccgg
    46981 gcgataaacg catcgcacag gatctggcgg aactggcagc agacaatcag gcttttttac
    47041 tggcaaatca tggcccagtg gtttgcggtg aaagcctgca agaagccgcc aacaatatgg
    47101 aagagctgga ggaaacggca aagctgattt ttattctcgg tgaccgcccg atccgttatc
    47161 tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa tgcctcgttt tgcagctaat
    47221 ttatccatga tgttcaccga agtgcctttt attgaacgct tcgccgcagc gcgaaaagcc
    47281 gggttcgatg ctgtggaatt tctgtttccc tatgactact ccaccctgca aatccaaaag
    47341 caactggagc aaaaccatct gacactggcg ctgttcaata ccgctcccgg agatattaat
    47401 gcgggggaat ggggattatc cgcccttccc ggacgtgagc acgaagcaca cgcagacatc
    47461 gacctggcac tggaatatgc attggcgctc aattgtgaac aagtccatgt gatggcaggc
    47521 gtcgtgcccg ctggcgaaga cgctgaacgg taccgggcag tatttatcga taatctccgc
    47581 tacgctgccg accgctttgc gccacacggc aagcgaatat tagttgaagc actcagcccc
    47641 ggcgttaagc ctcattatct cttctccagc cagtatcagg cactggctat tgttgaagag
    47701 gttgcgcgag ataacgtgtt tattcaacta gatacttttc atgcgcaaaa agtagatggc
    47761 aacctgacgc atttaatccg cgactacgct gggaaatatg cgcacgtaca aattgccgga
    47821 ctacctgatc gacatgaacc ggacgatgga gaaatcaact atccgtggct gttccgcctg
    47881 ttcgatgagg tgggatatca gggctggatc ggttgtgaat ataaacctcg tggcctcacc
    47941 gaagaagggc ttggctggtt tgacgcctgg cgctaattcg ttaaatccct aattacaacg
    48001 tacccataca tccccccatc tacggatggg gtaggttaac tataactttc agacagggtt
    48061 tcccatgtcc acaattacat tgttatgcat tgcgttagct ggcgtaatca tgctgttgct
    48121 gctggtcatc aaggcaaagg tacaaccatt cgttgctctg ctcctcgtca gcctgttagt
    48181 cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa gtgatgatcg ccgggatggg
    48241 cggtgtgctt ggttccgtca ctattattat tggtctgggc gctatgctgg ggcgtatgat
    48301 cgaacactct ggtggtgcag agtcactggc taattatttc agtcgcaagt taggtgacaa
    48361 acgaactatc gctgcgctga ctctggcagc gttcttcctc ggtattcccg tcttctttga
    48421 tgtcggcttt attattcttg cgccaatcat ttacggtttt gccaaggttg ccaaaatatc
    48481 gccactcaaa tttggcctgc ctgtcgctgg aatcatgcta actgttcatg tggcggtacc
    48541 gccgcatcca ggccctgtcg ccgcagcggg gttactccac gcagacatcg gctggctaac
    48601 catcatcggt attgcgattt ctattcccgt agggattgtt ggctactttg cagcgaaaat
    48661 aatcaatacg cgccaatatg cgatgtcggt ggaagtactg gaacagatgc aactggctcc
    48721 ggccagtgag gaaggcgcaa caaaattaag cgataaaata aatccaccgg gcgtcgcgct
    48781 ggtcacctcg ctaattgtta ttcctatcgc gattatcatg gcgggtacgg tttccgcaac
    48841 actgatgccg ccttcgcatc ccctgcttgg tacgctacag ctgatcggct caccaatggt
    48901 agctctaatg attgcgctgg tgctggcatt ctggttattg gctttacgtc gcggctggag
    48961 cttacaacac accagcgaca ttatgggctc agcccttccc actgcggcgg tagtgatttt
    49021 ggttactggt gctggagggg tatttggcaa agtgctggtg gaatcgagcg tcggcaaagc
    49081 ccttgccaat atgctacaaa tgattgacct gcctctgtta ccagccgcat ttattatttc
    49141 actggctctg cgtgcatcgc aggggtcagc aaccgtagca atcctgacaa ccggcgggtt
    49201 actctcagaa gcggtgatgg gattgaatcc gattcagtgc gtattggtga cgctggcagc
    49261 ctgctttggt gggcttggcg cgtcacatat taatgactca gggttctgga ttgtgaccaa
    49321 atatctgggg ttgtcggtag cagacggtct gaaaacctgg acagtgttaa cgaccattct
    49381 cggttttacc ggattcttaa ttacctggtg cgtatgggcg gtaatttgac cttaataaaa
    49441 aggtccgatg ggcatcggac cttttattta acgcttacct tccaccagca gcggcgggat
    49501 gctcggcacc attggcgcgt catcaatatc tttctgcgtc atgcggaacg cttcgcgata
    49561 atgttcgcgg ctggtacggc gcagcggttc ggtatcgcgc caggtataaa ggcaatgctg
    49621 gcactgatat accgtccaga catctttcac cggcgatttc gccatcactt caatctgttc
    49681 atcggcacaa cgtggacaaa tcatcttctt ctccttattt acgtgcagcc agcatagcgg
    49741 tcagtttttc agcccaggct ttggtttccg gtaaatccac caccggttgg ctgtagtgac
    49801 cacggttgtc cggggcgaca ggcgtagtgg cgtcgataat cagcttgtcg gtgatccccg
    49861 cagggcttga gcctggatcg agttccagca cggacatatt cggcaactgc accaaatccc
    49921 ctgccgggtt cactttcgag gagagcgccc acatcacctg cggcaggttg aacgggtcaa
    49981 cgtcttcatc gaccataatc accatcttca cgtagcccag accatgcggc gtggtcattg
    50041 cgcgcaggcc caccgcgcgg gcaaagccgc cgtagcgttt tttggtggag ataatcgcca
    50101 gcaggccatg ggtgtacatg gcgtttaccg cctgcacttc agggaactcg gctttcagct
    50161 gctgatacag cggcacgcag gtggctggcc ccatcaggta gtcgatttcg gtccacggca
    50221 taccgaggta cagcgattcg aaaatcggcc tggtgcggta agagacttta tcgatgcgca
    50281 ccacggtcat gttacgcccg ccggagtagt gcccggtaaa ctcaccgaac ggcccttcga
    50341 tttcgcgttt acggctttcg atgacccctt cgaggatcac ttctgaaccc cacggcacat
    50401 caaaaccggt caacggggcg gtggcgatcg ggtacgggct ttcacgcagc gcgcctgcca
    50461 tttcgtactc ggactgatca tatttcagcg gcgtggcccc catcagcgtg atgatcggat
    50521 cgttaccgag cgtgatcgca atcggcagat cttcaccgcg ctcttctgct ttatgcagat
    50581 gcagggcgat atcgtgcatc ggcaccggtt gcaggccgag cttacgcttg cccttcactt
    50641 ccatgcggta gatgccgacg ttctgcttgc cgaagttatc cgggtcgagc ggatcgcggg
    50701 aaaccacgca cgctttgtcg agatagaaac cgccatcgcc atcgtttaaa cgaaacagcg
    50761 gcaggatatc gaacaggtta atctcttcgc catcgacggt gttctgcgcc caggctggat
    50821 tggcgcggcg ctccggggca atcgggaagt tatcccagcg gcggataaac tcatcaatct
    50881 gctttttgac cggggtgttt ggtggcaggc caagggaaat cgcgtggttc tgccaggaac
    50941 cgatggtatt catcgtcacg cgggcatcgg taaagccgcg aatattatca aaccacagcg
    51001 cgggcgcgcc gtcgccgata cgcccggtgg cgttagctgc tgctgccaga tccggctcag
    51061 cgttcacctc ttcgctgatt ttcagtaact ggccgtggtc atcaagcgcc tgtaaaaagc
    51121 tgcgtaaatc atcaaatgcc attattcatt ctcctgagaa aaattccggg cctgcggcaa
    51181 tccttgccag cgcctggcgt ggggatgttc gaggccaaat tgatccagca cgcgggctac
    51241 cacatggtgg acaatgtcat ctaccgtttc gggatggtta taaaaggcag gcatcggcgg
    51301 caccatcgcc acgcccatgc gtgaaagtgc gagcatattt tcgagatgga tggtgctaag
    51361 cggcatttct cgcggcacca gcaccagttt gcggccttct ttgagcacga cgtccgccgc
    51421 gcgccctacc aggccatcgg cgtaaccagc gcggataccg gcgagcgttt tcatactgca
    51481 cggaataacg atcatgccgt cggtacgaaa agaaccggat gagatgatcg ccgcctgatc
    51541 cgccgggtta tggctgaagt ctgcgagggc agcaacatcg cgagcgctgt aaggcgtttc
    51601 cagttcaatg gtggttttcg cccacttcga catcaccaga tgagtctcga cattcggcat
    51661 ctcccgcagc gcttgcagta atgccacacc aagaggcgca ccggtagccc ctgtcatccc
    51721 gacgatcagt ttcattgctt atcctcttaa ttgtttgtat acgaacgtta ttattaacat
    51781 actccttcat ttccacagcg gcaaggattg aaaagtaaca acatcttctt cgtcactaaa
    51841 aaaccgctat gatagcaaca gagaattagc ccggagttta catggcgtta cgaaataaag
    51901 cgttccatca gttacgacag ctttttcagc agcacacggc tcgctggcag cacgagttac
    51961 ctgacctgac caaaccacag tacgcggtga tgcgcgccat tgctgataaa cctggtatcg
    52021 aacaggtggc actgatagaa gcagctgtca gcaccaaagc aacgttagca gaaatgttgg
    52081 caagaatgga aaatcgtggc ttagtcagac gtgaacatga ccccgctgat aagcggcgac
    52141 gctttgtctg gttgactgcc gaaggcgaaa agatacttgc tgcggcgata cccattggtg
    52201 acagcgtgga tgaggaattt ttggggcgtt tgagtggaga agagcaggag ctgtttatgc
    52261 agctggtgcg caagatgatg aacgcatagg gggcaaattt ccggatgcga cgctatcgcg
    52321 tcttatccag actacaaata caccgactca ccaaccgaaa gaaaaaaggc cagcctcgct
    52381 tgagactggc ctttctgaca gatgcttact tactcgcgga acagcgcttc gatattcagc
    52441 ccctgcgttt gcaggatttc gcgcaaacgg cgcaggcctt caacctgaat ctggcgaaca
    52501 cgttcacggg tgaggccaat ttcacgacct acatcttcca gtgttgccgc ttcgtacccc
    52561 agcaaaccga atcgacgtgc cagcacttca cgctgtttgg cgttcagctc gaacagccat
    52621 ttgacgatgc tctgcttcat atcgtcatct tgcgtggtat cttccggacc gttctctttt
    52681 tcatcggcca ggatgtccag caacgctttt tcggaatcac cacccagcgg ggtgtctacc
    52741 gaggtaatgc gctcgttaag acgaagcata cggctgacgt catcaactgg cttatccagt
    52801 tgctctgcga tctcttccgc acttggttca tggtccagct tatgggacaa ctcacgtgcg
    52861 gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg gcaaacgaat agtacgggtt
    52921 tggttcataa tcgcccgttc aatcgtctgg cgaatccacc aggttgcgta tgttgagaag
    52981 cggaaaccac gttccgggtc aaacttctct accgcgcgga tcagccccag gttgccctct
    53041 tcgataaggt ccagcaacgc cagaccacga ttgccataac ggcgggcaat ttttaccacc
    53101 agacgcaagt tactctcgat catccggcgg cgagagacga catctccacg cagtgcgcga
    53161 cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg aataaccaat ctcaccaagg
    53221 taaagctgag tcgcgtccaa cacacgctgt gtggctccct gcgataacag ttcctcttcg
    53281 gccaaatcgt tatcactggg ttcctcttct actaaggcct tttcgtcaaa aacctcaact
    53341 ccgttctcat caaattccgc atcttcattt aaatcatgaa ctttcagcgt attctgactc
    53401 ataaggtggc tcctacccgt gatcccttga cggaacattc aagcaaaagc atggttccgc
    53461 cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga tttccccttg taacgaattt
    53521 caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat ggttgctatt ttttgccccg
    53581 ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt atgggcgtag gcactcaggt
    53641 aatcatcatt atgtttgatg ataatcagat taccgtagcc gcgcagcgcg ttaccagcat
    53701 aaacaacgcg gccatctgcg gtcgcgataa ttgcctgtcc tttgctgcct gcgatatcaa
    53761 tccccttgtt gcccccctca gaagcgccaa aggtttcgat cactttgccc tcagtcggcc
    53821 agcgccaggt ggagataggc gtactggttg atgtactgct gacagttggc tcggttgtgc
    53881 ttgctgttgg taccgttaca ggcgctgtga ccgtggtcgc agctggcttg ttgttcggca
    53941 acattttgtt agcactctgt tcacccgaag attcagaata cgtaattgtc ggttgcgacg
    54001 caacagcaac ggtggaattt tgtgcaggct tgatcacaac gccttgctct gctgcgtcgg
    54061 cctgggtaat ggcatttccg ccagtgattg gcgtaccgga agcattaccc acctgcaagg
    54121 tctgaccaac gttcagcgcg tatggtgcct gaatattgtt gcgctgagca aggtcacgga
    54181 aatcgttgcc agtaatccag gcgatataga aaagtgtgtc gccttttttc acggtatagg
    54241 tactgccgct ataactgcct ttcggaatgt tcccatactg acggttatag acgatgcgtc
    54301 cgttttccat ctgtaccggc tgctgagcta ctggctgcac tggctggatt tgcggttgtt
    54361 gagtagcctg aatttgtggc tgctgtaccg gctgaatttg cggttgctgg gctgtagacg
    54421 tcgtccccat tttcggcggc ggcgtaatca acataccaga attggtattt gcaggcgcat
    54481 tgccattaac ggagctgacc ggtgccggtg gatttgaagt gtcagaacag cctgccagcc
    54541 atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc
    54601 tcatttatcc cccaggaaaa attggttaat aaccagtgac ataattaccg tgcaaggcac
    54661 cctactgaac actggaaaag atgttcacga tacgctgacc tgcggcaaaa taaccaggaa
    54721 aaatccaggt atttcctcac gttttaagcc agctcaccct tcactaaagg gacaaagcgc
    54781 acggcctcca cggtatcgat aataaattcg cctccccgac gacgcacccg tttcaaatac
    54841 tggtgctcct cccctacggg taagacgaga atcccgcctt cgtccagctg cgtcattagc
    54901 gcagttggaa tttccggcgg tgccgccgta acaatgatag cgtcaaacgg cgcgcgtgcc
    54961 tgccaacctt gccatccatc gccatgacgg gttgaaacat tatgtaaatc aagatttttc
    55021 aggcggcgac gcgcctgcca ctgcaaacct ttaatccgtt cgaccgagca aacatgctgg
    55081 acaagatgcg ccaggattgc cgtttgatat cccgaaccag tgccaatttc cagcacccgc
    55141 gactgcggcg tcagctcgag taattcggtc attcgcgcca ccatatacgg ctgcgaaatt
    55201 gtctgcccct gacctatcgg caaggcgata ttgtcccagg ctttttgttc aaacgcttca
    55261 tcaacgaatt tttcacgcgg cacggcggca agtgcattca gcacctgctc atcctgaata
    55321 ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat tgcgtgccaa
    55381 ctcccacgct gtttaaccag tctgaaacca catcttgcgc gctatgcgca gttaaatcca
    55441 catgcagcgg cgtgatggag acatagccct catctaccgc agcaaaatcg gtccccggac
    55501 cggcatcaca tttaccgccc ggcgggccaa tccagtacag cgtattgccg cgcggatctt
    55561 gctgcgggat cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg cgaatacctt
    55621 tgatttgatc caagggtaaa tccggaacgt taatattaag aatacgcccg gtgcgcaacg
    55681 gctctttaca cagtgcgcgc aaaattgaac aggttaccgc cgcggcagtg tcgtaatgtt
    55741 tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa atgacggcct tccatcgcgg
    55801 cggctaccgt accggaataa ataacgtcat cccccagatt cggcccggcg ttaattccgg
    55861 acacaacaat gtccgggcgc ggacgcatca gagcattcac gccaagatag acgcaatcgg
    55921 tcggggttcc catttgcaca gcaatatcac cattttcaaa ggtaaacgtg cgcagggagg
    55981 attccagtgt cagagaattt gaagcgccgc tgcggttacg atcgggggcg accacctgaa
    56041 cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat accgggtgca tgtaccccgt
    56101 catcattact cagcaatatg cgcataatca cctgttgtgt tgataagttc cctgacaacg
    56161 ctggttgcaa aactacccgc cggaagccag aaacggatct ctacggtgac gtcatcccac
    56221 caattccagc ttaattgttg cggatacagc aacatcgctc tgcgcgcggc ttcaactttt
    56281 tcgcgcacca gtaaagcttg taattcagtt tctgcggcga cagctgcctt ttcgaatgcc
    56341 agcgcttcac gctgagttcc ccattcgcca ctgcccggca atgcggcggt tatcatcagc
    56401 tctttatcgt tgacgcgacg ctgtaattcc gccagttctt cggtggttgc tacaaaccag
    56461 ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa cttgattaac gtctgctttt
    56521 ttgaggcgct cagcaacaat ctgattaaac aacgcactgc gggctgccga caaccaaaaa
    56581 ctccgtttat tgcgatcgcg caccggagta ttggtttgcg cccagcgcag cgcgccctgc
    56641 aagttgctac cgccaatccc aaaacgttgg gcaccgaagt agttcggtac accttttacg
    56701 caaatatcga tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg cagaaccagg
    56761 gtaaaggcgt tacctttcag cgcgcctaaa cgcagcttgc gcttgtgccg cgcatactcc
    56821 agcacctggc agccttccag ttgaaaggcg ctcagatcgg gcatttcctt gcctggcacg
    56881 cgagcgcata accactgttc cgtaacagca tgtttgtctt tttgcccagc gaagctgact
    56941 tcacgggcat gaattttcag gaatttcgcc agtgcatccg ccacaaaacg ggtattgcag
    57001 ccgtttttga ggattctaac cagaatatgc tcaccttcac catcaggctc aaagcccaaa
    57061 tcttccacca ccacaaagtc ttccggattg gctttcagca gcccggtgcc ttgcggttta
    57121 ccgtggaggt aagtgagatt atcaaactca atcattttgt tgccttaatg agtagcgcca
    57181 ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc aagtttttcc gtagtagtgg
    57241 ctttcacgtt aacatcatcc atatggcagc cgagatcttc ggcaataaac acgcgcattt
    57301 gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat agtgacatcg acgttgccca
    57361 gggtataacc cttcgcctga atacgacgcc aggcttcgcg tagcagctcg cggctgtcgg
    57421 cacctttaaa tgccggatcg gtatccggga acagcttgcc gatatccccc agcgccgccg
    57481 cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc gccatcagaa tgcgccagca
    57541 atcctttttc gtaaggaatg cgtacgccac caatgataat tgggccttca ccgccaaagg
    57601 catgtacgtc aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga tggttcgggt
    57661 gaggtaaaac tcggccagtg ccaaatcttc cgggcgcgtg actttaatgt tatccgcacg
    57721 gccttcgacc aactgaggat ggaatccgca atattccagc gccgaggctt cgtcggtaat
    57781 agtcgcgcct tcatttagag cgcgcgtcag acagtcatgt aacagctcac gagggaaaaa
    57841 ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa tggcattttt
    57901 gcccggttcg gcacgtttca tcgtatcgcg cactggtgcg gctaggatcc ctcccgtgcg
    57961 gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac aaggacgagc
    58021 ggcgtcatgc accaataccc actgcgcgtc gccagcggct ttcaaacctg ccagcacgga
    58081 atcggcacgc tcatcaccgc catctacaac ggtgatttgc ggatgattcg ccagaggaag
    58141 ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg acacgtttca cccggggatg
    58201 cgccagcagc gcatgcaccg agtgttcaag aatggtttga ttaccgattg agagatattg
    58261 cttaggacat tccgtttgca ttcgacggcc aaatccggcc gccggaacca cggcgcaaac
    58321 atccaaatga gtggttgcca tgttaattcc cgggctgatt tatcgattgt tttgccccgc
    58381 agactgtgcg cgcttcgacg cgtcaggcac cagacgataa aaagtttcgc ccggcctggt
    58441 catgctgagt tcattacgcg cacgctcttc gagcgcctcc tggccgccat tgagatcgtc
    58501 aatttcggca aaaagttgat cgtttcgcgc tttaagtttc gcgtttgtag cttgctgtgc
    58561 cgccacatca tcattgacgc gggtatagtc atgtataccg ttcttaccga accacagcga
    58621 atactgtagc cagaccagaa tagccagcaa cagcagcgtt agtttaccca tcctgccccc
    58681 tgaaaaacgg catcatcatc ccatgcatcc gaagacgact ctacatcctc tgttggggat
    58741 accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac ccagggcgtg
    58801 cagaacataa tctcattatt agttacggtt tgaattatga acagaggaga cgggaaagta
    58861 caaattagcc cagtagccac ataaacagtg cgccaaacat aatgcctact gtcaccaggg
    58921 tgaaaacaat actgtagcgt agctttccgt ccatcaatga atgcagcgca atccccacca
    58981 ctaccgcgac gggcatcagc gccagaaaga aaggccaggt gtagataaag aagaacagcg
    59041 tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa taaccaggag ataaaaccga
    59101 ccacggcacc aggcagtgac catgtggttt cttcatcctc agtaaggctg tcgttatttg
    59161 ttagtgtaat gttatggcta ttacgcatat ttgatcctgt tactttgacg aaccgggcat
    59221 ggaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag atctaataat
    59281 tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc cgattcaggc
    59341 gcttcgtaaa cagaatctat tcccgtaaag ttgcgcagtt caccggcacg cgctttctta
    59401 tataagcctt ttggatcgcg ggcttcgcaa atcgccagcg gcgtatcgac aaacacttcg
    59461 ataaagcgcc cttctcctac gcgttcgcga accatctggc gttcggcgcg gtgtggcgag
    59521 ataaatgcgg tcagcactac cagtccggct tcaaccatca aattcgccac ttcaccgacg
    59581 cgacggatat tctctttacg atcggcatcg ctaaaaccga gatcgctgca taatccgtgg
    59641 cgaacattat cgccatccag cagatacgta ctgacgccga gtttatgtaa cgcctcctcc
    59701 agcgccccgg cgaccgttga tttaccggac ccggagaggc cggtaaacca cagcactaca
    59761 ccacgatgac agtggtgtag ctcgcgttgt tgcacagtga ccggatggct atgccagacg
    59821 acgttttcgt catgcagcgc cattatttat cccccagcaa atcgcgcgcg ccccagtgcg
    59881 gaaagtggcg acgaaccaga gcattcaatt ccagttcgaa tgcactgaat tcagatggcg
    59941 cagcagttgc ctggctaact ggctcgtgca ccataccggc acccacggtc acattgctca
    60001 ggcgatcgat aaaaatcagc ccacccgtca ccggattttg ttgataacga tctaacacca
    60061 gcggctcgtc aaaagtgaga tccacgaggc cgatcccatt cagtggcagg ttttcaactt
    60121 cacgctgggt aaggttatta atatcaacct gatagcgaat gccatcaaca cgcgcgcgcg
    60181 tcttcttacc ggcaattttg atgtcgtaac tctgccctgg agaaagcggc tgttccgcca
    60241 tccataccac atccaccgac gcgctctgca ccgccggtaa cgcttcgtct gccgccagca
    60301 gcagatcgcc acggctgatg tcgatctcat ccgtcagcac cagggtgatc gcttctccgg
    60361 caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg cgcgacgttt gattccacac
    60421 cagaaggcag cacttttaca cgttgcccga cttccacgcg accggatgcc agcgttccgg
    60481 cgtaaccacg aaaatcgaga ttcgggcgat taacgtactg caccgggaag cgcattggct
    60541 gagcatccac cactcgctgg atctccacgg tttccagcac ttcgagcagt gtcggaccgc
    60601 tgtaccacgg catactttca ctttgcgaag ccacgttgtc gccttccagc gcagagagcg
    60661 gcacaaagcg aatatccaga ttgccaggta actgttcggc gaaagtcagg tagtctttac
    60721 gaatgcgggt gaatgtctct tcactgtaat ccaccagatc cattttgttg atcgctacga
    60781 ccagatgttt gatccccagc agcgtggaga taaaactgtg acgacgggtt tgatcgagca
    60841 cgcctttacg ggcatcgatc agtaagatcg ccagctcaca cgttgaggcg ccggtcgcca
    60901 tattgcgggt atactgctcg tgcccggggg tgtcggcgat aataaattta cgcttctcgg
    60961 tagagaaata gcggtaggcc acatcaatag tgatgccctg ttcgcgctca gcttgcaggc
    61021 cgtccaccag cagagccaga tccagttttt cgccctgggt gccgtgacgc ttactgtcgt
    61081 tatgcagcga tgagagctga tcttcgtaga tttggcgggt atcgtgcagc aggcgaccaa
    61141 tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc aggcttttat
    61201 gttgttgcgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt tgtgcaagtg
    61261 cggtgttcat cttaaaaata cccctgacgt tttttcagct ccatagaccc cgcctggtcg
    61321 cggtcaatca cgcggccctg acgttcactg gtggtggaaa ccagcatctc ttcgatgatt
    61381 tccggcagtg tttgtgcatt tgactcgacc gcaccggtca gcggccagca gcccagcgta
    61441 cggaaacgca ccatccgttt tttaatcact tcgcccggtt gcaggtcgat acggttgtca
    61501 tcaatcatca tcaacatacc gtcgcgttcc agaaccggac gttccgcagc gagatatagc
    61561 ggaacaatgt cgatattttc cagccagatg tattgccaga tatcctgctc ggtccagtta
    61621 gagagcggga agacgcggat gctttcgcct ttgttaattt gcccgttgta gttgtgccac
    61681 agctccgggc gctgattttt cggatcccag cgatggaagc ggtcacggaa agagtaaatt
    61741 cgctctttag cgcgggattt ctcttcgtca cggcgcgcac caccgaaggc ggcatcaaaa
    61801 ccgtatttgt tcagcgcctg tttcaggcct tcagttttca taatatcggt atgtttcgcg
    61861 ctgccgtgca cgaatggatt aatccccatc gccacgcctt ccgggttttt atgcaccagc
    61921 agttcgcagc cgtaggcttt agcagtacga tcgcggaact catacatctc gcggaatttc
    61981 cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa cgccttgcgc
    62041 gccaaatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac cggatttgag
    62101 aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg ccgcaggtga
    62161 gtaagtcgtt tttgatccat aaccgttcct ttgcaatacc gctattttct tgccatcaga
    62221 tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa tgagtagttc
    62281 cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc accggatttc
    62341 gggcgtttag gaagatttga aattgtttta gcgcagcggc agtttcatac tatggcggta
    62401 aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc accgtaccgc
    62461 tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcac ctaaacctgg
    62521 cgattttgct aatacccagg cacgacatat tgctactttc tttccgggac gcatgaccgg
    62581 aactccagca gaaatgttat ctgccgatta tattcgccaa cagtttcagc aaatgggtta
    62641 tcgcagtgat attcggacat ttaatagtcg gtatatttat accgcccgcg ataatcgcaa
    62701 gaactggcat aacgtgacag gaagtacggt gattgccgct catgaaggca aagcgccgca
    62761 gcagatcatc attatggcgc atctggatac ttacgccccg ctgagcgatg ctgacgccga
    62821 tgccaatctc ggcgggctga cgttacaagg aatggatgat aacgccgcag gtttaggtgt
    62881 catgctggaa ttggcagaac gcctgaaaaa tacgcctacc gagtatggta ttcgatttgt
    62941 ggcgaccagc ggcgaagagg aagggaaatt aggcgctgag aatttactca agcggatgag
    63001 tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa ttgttggcga
    63061 taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat taacgcgcga
    63121 cagggcgctg gcaattgcgc gcagtcacgg aatagccgca acgaccaatc cgggtttgaa
    63181 taaaaattat ccgaaaggca ctgggtgttg taatgacgca gaaatattcg acaaagcggg
    63241 cattgctgta ctttcggtgg aagcgactaa ctggaatctt gggaataagg atggttatca
    63301 gcaacgcgca aaaacagccg cattccctgc gggaaatagc tggcatgacg taagactgga
    63361 taatcaacag catattgata aggcacttcc tggaagaata gaacgtcgct gccgtgatgt
    63421 tatgcggata atgctaccgc tggtgaagga gctggcgaag gcgtcttgat gggtttgaaa
    63481 atgggagctg ggagttctac tgcaggggcg gggaattcta agtgatatcc atcatcgcat
    63541 ccagtgcgcc cggtttatcc ccgctgacgc ggggaacaca aaaccaaact tctccataaa
    63601 ttccatagcc gcggtttatc cccgctggcg cggggaacac gagtctatca gcgacactac
    63661 cggcaatagc gacggtttat ccccgctggc gcggggaaca cctatagcgc cacgttccga
    63721 gcgctgcgag ctgcggttta tccccgctgg cgcggggaac acctaatgag tcagtttgaa
    63781 cagatccccg acaacggttt atccccgctg gcgcggggaa cacggcttta aaattgtgtt
    63841 cccgtctggc gctgggggtt tatccccgct ggcgcgggga acaccaaaat attacgagct
    63901 tcgtcaggcc atggaccggt ttatccccgc tggcgcgggg aacacactaa gcatacataa
    63961 ctgtttttaa acaaatttat tccgcatcaa caatctacca actaaattaa aacatttcct
    64021 tatttttaaa gaacggataa cttattgatt atcaacagga agaaaagaaa ccaaacgtaa
    64081 cccatccaaa tccaccggaa tacgtctgtt ttctccccag gtctgaaatt caaaacccga
    64141 ctcggtattg gtcgcccagg ccatcaccac atttccgcaa ccagccagtt gggtaatttg
    64201 ctgccagatc atctcccgaa tacgttttga tgtatcacca acatacacac cggcacgcac
    64261 ttccagtagc cagattgcga gccgtccacg taagcgcggc gggacatttt ctgtaacaac
    64321 cacgaccatg ctcatcagcc gcgtcctcga tgaccgctat cgcccaacgt ttcaggttcg
    64381 gggatggctg gcggtaacat atccggcgca ggttgtggcg gttcaatttc acctgcggca
    64441 aggacttcct caattaacgg tattaatttg cccgttaact tagtgctacg gaaaatatcg
    64501 cgacaggcta atctgacttc tttatcaggt tctgcgggtt gcctcgctgc tatttcaaat
    64561 gcctttggca caaccgaatc aaatttaatg atatcggcta tgtcataaac aaatgaaagc
    64621 ggtttgccac tatggataaa cccaatagcg ggcgcatatc ccgcggctaa tactgccgct
    64681 tcagatatac cgtacagaca tgatgtagca gcactgatgc agcgattcac aacatcgcct
    64741 ttttcccagt ctttaggatc gtatttgcga ccattccatt tcacaccata ttgtttcgcc
    64801 agtaatgcat acgtctggcg aacgcgagat ccctcaattc cacgtaactg ctccactgaa
    64861 cggcgagctg gctgtggttc acgaaaacgt aattcataca ttttgcgcac caccttcaac
    64921 cgtagatctt ccgttaaagc cagctttgcc tggtagagta atttatctgc ccgcgcccca
    64981 ccgggttgtc cggaggagta aacgcgaacg cccgcttcac cgacccagac cagcagtgtt
    65041 cccaccgtgg cggccagatg caccgccgcg tgggaaactc tcgttcctgg ttcgagcata
    65101 atgcaggcga ccgatcccac cggaatatgc gttcggatcc ctgttttgtc gatcagcacg
    65161 aaagcgccgt ccagtacgtc gatctgaccg tactggagga agatcatgga ggtgcgatct
    65221 tttaatggga tcgggctcag tggtacaaac gtcacgcctc tgctccgggt ttgatcagca
    65281 tcagaccaca gccgaacgcc cggcttttac catacccctg gctcaggcgt tgcacaaata
    65341 acccaggatc ggtgaccgta agcatccccg tataatccac gctactgaac tggatcagtt
    65401 gccgggagtt ttcccgccgc agttgctgtt gtcggtaggc atcaaccgag gtatccagca
    65461 gtgtaaaacc gcttctctcc ccctgcgctg ccagccagtc cagcgccgcc tgttgttgat
    65521 gcaaccagac atcacttcct tccgcctgcc ccctcacctg ccgtttcgcc tccatcagca
    65581 gatcgtggcg cttgcccgct ttacagattg ttggattcgc ccgcaggttg aaacaaagtt
    65641 gttgtccggt acacagctcg ggcgcaaatg accggcattc tatggtgaac gtatcacttt
    65701 ccgccgggcg ttcctgcgac agtacaaaaa agcgaaacgc gccctggagt tcttcccgac
    65761 gataaagaaa ttgcctttct ttgccgccag ggaagagatc ccacagccac tgatgcatca
    65821 catattcacc gcgatccacc agatgcagca actgcgcagg cgaaagctgg ccggtatgta
    65881 aggttattct tgagaggtac atggttcctc cttgctgagc cacggcccct gattgacggt
    65941 gcgttcccca aacagccact gctgacgatt taaaggaaca tcccggcggc gtaatatttt
    66001 gctcgcgacc aggccgtcgt gtttcccttc ccaccagcat tcatcctgaa gtttcgggag
    66061 tgagactttc agtttatgga aatgatcctg atactgttgg tatgcattac ataaggcatc
    66121 acacgcgttg ccttcgagca gtaacggcgc aagaggtaat gccagcggat gactttttcg
    66181 ccccagataa agcggaaaaa ctggatggcg taaaccgtcc tgcaactgtt cgaggctgta
    66241 aggtgcatcg gctgttgttg ccaccgccac catccaccag gcatcggtgt agtagtcgcg
    66301 ccgggagata atcgcgctca aaagctcagg gtcgctcaac tcttcgcgac ggctgaaata
    66361 acgtgcttta cgcacctctt ttggcatctg gaccgtgtga taatcccgtg cccagcgcgg
    66421 gttacggctg gcgcaaacca ccagtgaata gtggcgatta aacgcgttta agcgttcggt
    66481 atcatcacgc cgaatcccta ccccggcagc cagtagcccc agcaatgctg agcgcgaagg
    66541 aagttcatgg gtatgacgaa cttcacctgg agcatcgccc ccccaggacg ccatcggccc
    66601 atgaagctga aaaatcagat attggctcat aagccgcccc ttacgcgcag ataaagtcca
    66661 gcacgtcctt catgcttccc tgtttgttca tcacgtcaaa acttgcgcat tcggtcttct
    66721 gttcgtagac cgtattcatg ttttcgcgaa gcgttgtaat acgctgcacc gccacatcta
    66781 actgacgtgt gccattaatg ggttcataga aagccgccgc cagagagcgt ggttgatcgg
    66841 tgcctttttc tgccagcgcc caggaggcgt aggcacggct ggcaaagctg ttctgtttgc
    66901 cggttgggga gactttaagt gcggcttccg taaaggcacg tatggtctga ttagctaacg
    66961 cttcgtcacc gccgaggttt tctaccagca gatctttatc aatgcagata taggtgtaga
    67021 acagcgcaga accgaatccg gtttcatcta catgaccggc accggcatct tcagaagcct
    67081 ggcgtaaatc atctacggcg gtgaaaaaat cattttcgac aatcgtttca ctgacaccaa
    67141 acgcatgcgc gacctggcag gcggcttcta cattaaaccc gggttttttc gccagcatac
    67201 gaccaaacat agcgatatct accgccatac ggtctttacg taacaaagcg agatctttct
    67261 cttttggcgc gcgcttttct tctgccagtt gatgggccag cgcttttacg gcgtcaaatt
    67321 ctgccgggct gatatggacg agctgttcag tttcggcgct agtgagcgga tcttttggtt
    67381 ttttgtcgtt tttttgtttt ttgtcttttt tggctttccc aagataatcg gctatctcta
    67441 ctgcccattc gatggctttt ttatcttcga ttcctttctc aatcaggata gttgccgcct
    67501 cacgcgcaat acgcccgcta cgaacaccaa tatggcccgc cagcgcctgt tcaaaaagtt
    67561 cagaagtgcg ccaagcacgc ttcagacttt gcgaggaaac gcgcagacgc gttgctccac
    67621 ccagaatcac agttttcgga gagccggaat catcacggtt aagattggca gcagggtaag
    67681 cggttaacaa atgaagctga ataaatgttg tcatgagata gtcctttatt aattttgttc
    67741 gttatcggcg tcgccagcct ggtaatattc cagcgcccag cggatacgga taaattctgt
    67801 tggtcgctgc tggcggcggt gatgattgag caggtcatcg ctctcctggc accagcggaa
    67861 gaccccctct gccagagagt caagattgac ggaaccgttt aataatttga ccgcacggcg
    67921 taactgccgc aataaatcat ccggcgtttt tactgccaac aaacgagtaa accgcccctt
    67981 tttcattacc gccgccagtt gcgcggcaaa aggctgccgt tcgtcaatag ctttaacatt
    68041 ggcgctaact gctgctatca gcgctagtgc ggtgatacgc cactcgggtt catcctgcca
    68101 ctttatttgt ctgttcttaa gaaacaggcg aaatccatcc gtcagacaaa catcattaac
    68161 cgtcgtacta cgccgcagac tggcacgctc gccgcgttta tcctgcaact cttcatgcca
    68221 tttacgcagc gcaactttat gctcatcttt tacaatactc attcagcagc ctcctgcttt
    68281 ttctccctgg cggcgttagc attttgcttc tccgctgatg ttgtaaaata tttcttgcgt
    68341 gcggtcatga cgcgttccaa atcaacgggc tcatatggat tggtaaagac acgatcgtca
    68401 aaatcctgac gcgcgaataa ccacatttcc ttttgccatt tactgagtaa ttcgtacgga
    68461 tcctgacctt cttcaatttt gcgcactaat ctcaggaatc gatgctgagt tttgttccag
    68521 aagtcaatat ccacaaaacc gaaatcaagc tttgtggttt tcttctcaga gaaccatgct
    68581 tctttcaacg ctcggtgtaa caagcttaga atgtgtgatg ccgcttgcgc tgccagacgc
    68641 aacttaggta ttaggtcttc ttttttgttt aacaacagcg ggaaatggtg ttcataccaa
    68701 caacgaacct tcatatcttc aaaatcaaaa ccaaatcccc ataagcccac ccttgtctgc
    68761 tttagattgc tggcattcaa aagtttcacg acctgtgcgg ggagttcagt attgtttttt
    68821 gagttaccta cctctatgag gcctaaccag tcgcgccaga ttaaaccacc aggttgtggt
    68881 ttaactgagt aaaaatctcc accctcttta agcggcagac ggtaaggtgt tagaggatgg
    68941 cgccacatga cgtattgaac accatagttt ttgagtgaca tcaagcccaa aagagcatcg
    69001 ctctgcctac cgcaaatgtc gcagttacct attgaggttg tcttaaaatc aatacgtata
    69061 cgtcgtggca ttccccagta ggcctgaagt ttattgactt gttcatccgt taccacagca
    69121 ccgtccagtt cgctggtacg cgtcggtgca agccatggaa agaccaggtc atcaaaggtt
    69181 tttggtaaag gtaaatcggc ttcatcttgc ggcattacgt ttagccacag ctttcgccac
    69241 aaaggggttt gttgattgcc ctgatactcc agcaattcta taagtgtcgt cagaggccct
    69301 ccgccacgta gcccaggata gtaaccgcgt ccacctatag gtgcattcaa ctggattgaa
    69361 aaaagcgcta aaggcacaca atgcaggcac agatgctgag ttgtgtctcg cttaataaaa
    69421 tggtctgtat ttcgttcttt tgtttgtttt cctggtgcgt caggaagaag cgaagcaata
    69481 gaagttgcct taacttttaa ttcatcaaaa tcctgcataa atgaagggga atctggccca
    69541 aactgaaaag catgttctaa agacagcaat gcttccctta gcttttcagc ttccagtccg
    69601 tcttcccaga tatcatccca acgacgttga tcttttggcg cgaagctggt ttgtagtagc
    69661 cccaacaaaa actgccaggc cgccccctgg agatctgccc gcggcgcagc gatatcgaca
    69721 acattttcat cagccagatc gactggtgcc agtttgcctg tcgttccgtc tttaaaacga
    69781 acgggcaacc acggggttgt cagaagtgaa aacgagttca tacgcatcct ctcgtaaaaa
    69841 agaccatccc aggtcggttg tccatccttc catccttttc tgtatatcaa ctattcagag
    69901 agtcctgtcg cagacatctc aaccaacctt gccaatcaat ccctccctgg ccgaataccc
    69961 gcaactttcg tcatcggtga ctaaaatcac gtttgccatt tccggatctt gccgctgttc
    70021 aacgcaccac tgcctgaacg catccccttc cagtaaagaa aactcatccc gatgtttttt
    70081 ccaccagctt cggcgcactc tgacaacgct catttcccat gcgtgagcac cggtggcata
    70141 aggcttcacc acaccgtcaa tacaggtagc cagccacagg gaaacagact cttcagccag
    70201 acgtgtcgac agtttttccg gaaggtaatc gttgatattg gcggcatagc cgggcctgaa
    70261 gttcagcaca tactttttag cccttgcgcg atcacagtaa tatttgccca cttgctcctg
    70321 ctcgctgcgg gcaaatcctt ccggcattac cacgtcctca ccgtaaactg attcaataag
    70381 aagccgggct gcgtgtggca tctgaatagc gccttgctca cgcagcacac gctgcgtcag
    70441 ccagattcgc ccatgatcgg gatagacata tgcactgtta cgcatggcac tgccgaacca
    70501 ttcgtcacca ggagcgtcat cccagacggg ggccagaatc agcaattcag ggggggaacg
    70561 ctcgtctttt ccgtcacgct ttaactgacc attaatatcg cggatatgcc gctgtaatcg
    70621 ccccgctcgc tggattagca aatcaatggg ggccaagtcg gagatcattt cgtccaggtc
    70681 aatatccacg ctttgttcaa ttacttgtgt acaaacaatg acttgtgacg ctctctgtaa
    70741 actacaatat ttaccaaagc gtgccagcgt ttccgtttca attcgctggc gatcgctaaa
    70801 agcaaagcgg ctatgaaaaa gtgaaaggct ggaagcagga atgacgccgc gggcaagcag
    70861 ctgacgatac acctgaatag cgtcatcaac agaattccga atccaggcga tgcatttccc
    70921 ctgacttacc gccgattcga tacgcgcaat acactcttgt tcactatgga gccaacccac
    70981 gctcacgcta cgttcaactt ctttgcgcgt cgctacccgg tgcgaatgta catcggtttt
    71041 cgtgacatgc gtcagccagg ggtaatcatc ttttcccagg agtggggctt cttgctggcc
    71101 ctctgcacca cgcgcaaagg cggcgacgag tttgtcgcgc tgctgttgag ataacgtagc
    71161 agaaagcaaa ataacgctgt ttccgccacg cgcctgccgc tcgatcagcc cttcaagaat
    71221 gcacgacatg taagcgtcac aggcgtggat ctcatcagcc agcaggattt tgttactcag
    71281 ccccagaagc cgcagattat tatgtttaaa cggcatcact gccatcatcg cctgatccag
    71341 ggtgccgacg ccaatttcag ccagcaacgc cttcttgtta ctgtcggcaa accaggccgc
    71401 acaaccctgg ctgaaagctt gttcatccgg ttcttctgac ccgattaaat caccggacca
    71461 gagtgattca ttaaagcggt ccattaatgt gcgggcactg tgtgccagca ccaggctggg
    71521 gcgagattct ggcgaataga aagaaagcca ggttttgacc agccgatcgt acatggcatt
    71581 ggccgttgcc attgttggca gaccaaaaaa cagcccctga gctttccccg cagctatcaa
    71641 cctgtgcgcc aggataagcg ccgcttctgt tttacctgcg ccagtcacat cttccagaat
    71701 aaataactgc ggtcctggct ggctgatatc cagatccagc accttttgct gtaacggtgt
    71761 cggattctca ataaaaggaa acagtgtatt aattccggtg aaaggtgcgg tttctgcttt
    71821 tggaggaaag acggttaatg cgttttgagc ctgcactaaa gccttctgcc agtaatgttt
    71881 aatatccatt gcctgtgcga cgcgtggaaa aaatcgcgtt gacgaacctg tccagtctgc
    71941 gagtacgacg gttgcagaga tataccagga aaggtgtttt ataagttcaa tgccctcgtc
    72001 atcatcccag aatgcgggga tctctattcg cggaaacagt gccttgattg caaggagaaa
    72061 atctcgtgct gcagctttgt cttcaggcag aaaattatcc aattcatcca tacgatcagg
    72121 gggccgacca tgatgccctg tcgtcacggg catccacatt tctatcactc gcctaagttt
    72181 gcgcggagag agcgaagatg aaggaagtaa ctcctgacat tcacttaaat aatgattcca
    72241 caaccagtag cccagcgttg aatgagagat tttttcgtaa ttttttctgg cactttcctg
    72301 aatcttgagt tcagggggca ggtaaagttg ttgaaaagag cgggcaaatt ttccaatatc
    72361 gtgccagcac agcaaccagg cgaaaaattg agccgcctgt tccttgtcag aaatccctaa
    72421 ttgacgaaaa taatcagcca gcccgaagca atttctttta accattaaat agcccatcgc
    72481 ggctacatcc agcgaatgcc agcaaagaag gtgatagtcg tcgcccccgt ctttctcgcc
    72541 gcgtcgggtt tttccccaga aatcaaaaaa agtcacaata tttttatcct tcagtaaact
    72601 taaaggatat ttacgcatat tttaaaaatt atctgtgata tatatcagcc aataaaacat
    72661 ttacattatg aataaattaa tgattttcat ttgaaattca taataataaa tagagactat
    72721 atattcacat aaaaaatata aattttaaaa aagggtgcag gagaccaaac catcacaatt
    72781 cagtacgcgg ggttttaccc ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg
    72841 ccttaaactc cataccacgt tctctgatac tcaactcaca caacgagtca cctaccatca
    72901 gcgtgaatcc cagtactgta caacacagta cgatgattgc cacaagggca tattttgtca
    72961 gcatgtcttg cctccctgca aagaagagac taacattcga attgtttggg ttcgaacgta
    73021 agcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc
    73081 ttaacgccag acagcctcaa gcacccgacg ccattctata cctgataatt cttccagcgg
    73141 cgttcacgtt taaaatcagg aatactgctc gcaaaataaa aacgccccca tcatttctga
    73201 cagaggcgtt taatttatcc ggcaatattt acccttcgtg caatccacat tcccttttta
    73261 agccaaagaa acgtgtttct tcttccgaca tgccaggttc ccatttacgg gttgtatggg
    73321 tatcgcccac cgataaatat ccttcgtccc ataatgggtg atatttcagg ccatgttttt
    73381 gcagatactg atatatcgtt cggttatccc agtcgataat cggcagcact ttaaatacgc
    73441 cacgctgaat tgccagcacc ggtaaattgg cgcggctgcc ggattgttcg cggcgcaggc
    73501 cagcaaacca cgtttgcgca ttcagttctt tcagcgcccg gttcatcggt tcgactttgt
    73561 tgatttcatt gtacttttca atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg
    73621 cttcctgcca ggcggcgctt tcggtagcgc ggtacacttt caggttgagc ttgagtttgt
    73681 ccgttaactc gtcaataaag cggtaggttt ccgggaacag atagccggtg tcggtgagga
    73741 tcaccggaat atccgggtga atctgattca ccagatgcag gctcaccgcc gcctgaatgc
    73801 caaagctgga agaaagtaca tattcaccgg gcagattatc cagcgcccag gcaacgcggc
    73861 cttcagcgtc cagtttttcc agttcggcgt tagtttctgc cagcgccaga atgcgatcta
    73921 ccttcggcag ttcgttcagg gcgtttagat cgagtttgga catagattcc tcactgtttg
    73981 ccttgcctga tgcgacgctc acgcgtctta tcaggcctac aagaccgggc tgatggttaa
    74041 tcccacaaat cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag
    74101 tcgccgaagc cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc
    74161 gacgccagga tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca
    74221 atgcggttgc cgccaagatg cagattgtag cgacccggtg ctttacccac caggcccact
    74281 tccgccagca tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc
    74341 tcatcgctga caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg
    74401 aaacgctctg cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag
    74461 ttttcacgct gcggcgtgac ggcattcatt aaaccgctct ctttggcgat cttctcgatc
    74521 ttcgctttct cgctttccgg tacaccggcg atgatcagat tctggttcgc cgtaatgcgg
    74581 aaatcgcctt tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgccccgga
    74641 taatcaagga tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc
    74701 ttaacccagc caatacgatc gcctcgtccg gtgaactcat atggacggat cggttcaaat
    74761 ttgatccccg cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc
    74821 gtgtatttgg ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc
    74881 acgacggctt ccgccaccgc cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc
    74941 gtgcgggcgt aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg
    75001 ttgaagccta ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc
    75061 agatcgatat cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag
    75121 gtctgaccaa ggatcggttc ttcatcagta gtggcgactt tttcctggtc gagccagatc
    75181 tccgcatacg cgcgggtacg aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac
    75241 gcttccgcgt gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg
    75301 tcgttagctg tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg
    75361 acgttctttt tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg
    75421 ccatagatgg tgttttcacc ggcaaatttg tcgatcgcct gccactgttt agtggtaatc
    75481 accccacccg gcagacgaca gcgaagcagc atcgcgtggc gcggctccag cttctgttca
    75541 gcacgttcgg cgcggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc
    75601 aggaagttat cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg
    75661 ccgcgcaggt agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc
    75721 actaaaggcc ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta
    75781 cgcgcagctc acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa
    75841 tcacttccag aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat
    75901 aaatgtgggc accatcattg atccagcgcc acagctccgc gccctgttcg cgcagtttgt
    75961 cttgtacgta aactttttct ttttggtcgc gcgaccaggc aagatcgata cgtgtcagca
    76021 cgccatcttt gacgtagcgc tgccactcca cctggtacag gaagtcttcc gtaaagtgcg
    76081 gattaccaaa gaacagccag tttttacctg gcgcttcgtc ggcggcgcgt tgctgcataa
    76141 aggcgcggaa cggcgcaata ccggtgcctg ggccaatcat aatcaccggg gtttccgggt
    76201 tagcgggcag gcgaaagtta tcgttatgtt cgataaatac gcggacttcg ccctcttctt
    76261 ccacgcgatc cgcgaggaag ctggaggcac caccggcacg ggctcggcct tccacgtcgt
    76321 aacgcaccac accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcga
    76381 tggaatacag gcgcggggtc agcgggcgca gcagattaat cagcgcttcg gcatccagtt
    76441 gcgccggaga gaaacgcacc atgtcgacaa tcggcgtcgt cgcggcgtaa tgctgtaact
    76501 tcgctttatc gcccaccagc ggcagcagtg tttcactgcg ggtaagcgtg gcgtaattct
    76561 caacaatgtt ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag
    76621 gcaacgtttt gccctcgacg gtgacaggtt catcgccttt cagccacagc agttcgacaa
    76681 gttctttcac cagtgccgga tcgttctgat accagacgcc cagcgcgtca cccggctggt
    76741 aacgcaggcc cgagtcacct aagtcaattt cgatatggcg aacgtctttt tcagagttac
    76801 gtccggtaat tttctggtta accgaaaggc tcgccaccag cggagcgtct ttgctgtacg
    76861 ggctggagtg gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag
    76921 gcgcacgcga tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt
    76981 attcaacatc ggcatcgaca cggtcgagca ggcgttcacc gcccagttcc gccagcttgc
    77041 tgtcaaaatc tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca
    77101 cggcaaatgc ggtgttttcc agcttcggcg cttttttgga gaacaggaac ttatgctgcg
    77161 cgacggcttc ttccggcggt tccccttctc cttgcgttga cgtcaccacg ataagcagtt
    77221 tttcgctggc gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt
    77281 ttgctgctaa taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg
    77341 aggcggagat aatagttata cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg
    77401 cagcaggctg ctgattgagt acgccccaga aatagccaga aacccaggca agctgggtgg
    77461 gagttaaatc ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca
    77521 acgcggaagg tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt
    77581 ctgcgccctg tcagcgccca taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg
    77641 tggattaaag acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata
    77701 gtctttaaca acaaaataga ttaaccaacc taatgaaaaa caaatgaatt tagccaatca
    77761 ttaagataaa tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc
    77821 tttccggtac tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt
    77881 atttaaagat ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa
    77941 atgtggtcgc ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga
    78001 tccgcatacg ggctggatta tcgatttcgc tgaactaaaa gcggcgttta agccaactta
    78061 tgagcgcctc gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga
    78121 agttttagca aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt
    78181 gatggtaaaa gaaatctgca ccgctggttg tatctatcgc ggcgaatgat aagagtgtgt
    78241 cggcggtcaa tttcccttaa gtaacgctat gttagggtgt tgtgttctgg atatctgggg
    78301 catgacatgg aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct
    78361 tgcgcgttac gctgcgcgcg agcgggttta tccgttttgt tactggaacg cgctgaaatc
    78421 cccggcagca aaaatctttc cggcgggcgg ttatataccc atgcactcgc ggaactcctc
    78481 ccgcaatttc atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg
    78541 ttaacgcccg atggcgcaac gacgttttcc agcttacagc ccggcggtga atcctggagt
    78601 gtattacgtg cacggttcga tccgtggctg gttgccgaag ccgaagaaga aggtgtcgaa
    78661 tgcatccccg gagcgacggt ggatgcactg tatgaagaaa acggcagggt gtgtggtgtc
    78721 atttgtggtg acgatattct ccgtgcccgt tatgtggtgc tggcagaagg tgccaacagc
    78781 gtcctggctg agcgtcacgg gttagtgact cgtcctgctg gtgaagcgat ggcgttgggg
    78841 atcaaagaag tgctgtcgct ggaaccatcc gctattgaag aacgttttca tctggagaat
    78901 aacgaaggcg cagcgttgct gttcagcggc aggatctgtg atgacttacc cggcggcgca
    78961 tttctttata ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt
    79021 acgcaaagtc gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg
    79081 cgcccgctta tcaaaaacac ggaatcactg gagtatggtg cgcatctggt gccagaaggt
    79141 ggcttgcaca gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg
    79201 ttgcgcagtt gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc
    79261 gcgcaggcgg cggcacaaac gctgataagc gcctgccagc accgcgagcc gcaaaatctg
    79321 tttgcgcttt atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat
    79381 cagcatgttc cggcgctttt gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg
    79441 caggatattt cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag
    79501 ttattctggc gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg
    79561 agtctgcgat gtctgtagcc cataatctct ggcgcgttgc tgatgcgccg cacattgttc
    79621 cggctgactc cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggcaggtc
    79681 ttttttcgct cacaccggaa ggtgacttac gcgttgatta tcacggctgc ctggagtgtg
    79741 gcacctgccg tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg
    79801 gattcggcat cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac
    79861 ctgctccgcc agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggca
    79921 atcgactctg aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat
    79981 atcgttaaaa agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa
    80041 ggcgcgtcaa ataaagaagt cgtgattcag tttttgaaac tggtgaccga ggcggacggc
    80101 attatcagta ccaaagcctc aatgttgaaa gcggcaagag cggaaggttt tttctgtatt
    80161 catcgcctgt ttattgttga ttcaatttcg tttcacaaca ttgataagca agttgcgcaa
    80221 tcgaatccgg attgtattga gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg
    80281 acagagaaaa tccgccaacc gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg
    80341 cgtaatgcga ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg ggtctggacg
    80401 ttagcgaaaa aattactttg acgggataat cgtaaccaat tgaatttggt ttgatttgta
    80461 ggccgcacgc cacatccgac attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc
    80521 ctacaagtcc ccaggccaga accgtaggca ggatcatccg gcaacacgtt attggcaatc
    80581 tgcgtgaatg tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgccaac
    80641 atctgcctgt gaaaataccg cagcgctggc gtcatgatta atcgccacca caaacttgct
    80701 attgcgtacg cccgccatta acgctgctgc gccagaagcg ccaacaacaa tacacacctc
    80761 aggtgccagc agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat
    80821 cactcgcgcc cgactgtagc ccacttcggc tcccagcttt tctgccagca tagcgatttc
    80881 ctggttatct gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg
    80941 gtcgcgagta acatttttca gatcctctgt gctaaccagc cagtcgggaa gcgcacccgg
    81001 gacaatgttc agttgttgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg
    81061 acgcgccagg gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt
    81121 tccccagtgg gatttgcgca cgcttacggt tggaatatct aacgaagtca cctggcagat
    81181 gcttgcacca tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg
    81241 cggaaagaga acaacgtccg caggcgttcg ctgccactgt tccaccaacg catccagcac
    81301 ctgttccgcc actacaggct gaggttcaat ctgccagtgc gccagggtac aaccgctgaa
    81361 atcctgcgcc gccagccagc ttgctattgc ggcattctcc tgatttattg tcacaattgc
    81421 gatattcatg gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct
    81481 gctaccgttt gcccatcaat cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc
    81541 agacactgca tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc
    81601 gcagccattc gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg
    81661 gcaatcaccg caggcaagcg aacccggcaa caacgcaacc catgttcagt acgctgttcg
    81721 agggtgataa acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc
    81781 agcatttccg ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg
    81841 gtaattatca gatccagcgg attctgatgc tgccattcgg caatatggcg agcgacaaat
    81901 tccggcgcaa agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga
    81961 gccatgagat agcgcagcca gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa
    82021 gacatcggcg taccgttttt tctctgcgcc aacagcagcg cggcagcggc ctgttcatca
    82081 gcaccgagta aacttcgcag tagcgaaata tccggtccgc ttttaccctg agccgccgcc
    82141 tgccactctt tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg
    82201 ttcattcgca cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc
    82261 ccatagccag gtcacatcaa aaccaaccaa caacactgtc gccaggagca ataaggtgac
    82321 ttgcattccc cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag
    82381 tcgactcatg gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc
    82441 cgcaggcaaa atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa
    82501 aagcagcagc gttaatgaac tgccggaagg caaacaggcc atgactacca gcgttgccgc
    82561 cagcagcaaa aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa
    82621 tcccaacagc gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc
    82681 ttccagacca atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat
    82741 tacgaggcag acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa
    82801 caaggttttg atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg
    82861 accaaaatag cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca
    82921 gcgtggtgat tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc
    82981 cgatgccagt agccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat
    83041 acttgccagc acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg
    83101 atggcggcgc ggggaaaatt cagccagcaa ggtgtgacct actgaataat cgcctcccag
    83161 actaatgcca atcaaaatgc gcagtccaat aagatgctct ggcgtggtgg caaaaaattg
    83221 taaaaacgaa gcaagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat
    83281 atggtcggag atccacccaa gaaccaggct gccaaggaac aaaccaaggg gcgccgagcc
    83341 gccgatcatt cccgccataa acggcgtcag tttcatggcg ggcgtaagct gaataatggc
    83401 gtaaccaatg acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc
    83461 aatgcggcag tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat
    83521 ctttacccct tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca
    83581 acgccccgtc atcgcggaac gctgcctgac acattatttt tcaatcggat agatagtacc
    83641 cgtattcata atgccattag gatcgaactg ctttttcagc ccttccagca acgcccacgc
    83701 gctgccgtgt tccagtttgc tccagtgaac gcgatgttta ccgataccgt ggtggtgcac
    83761 catcgaaccg ccgaggcgaa tggtttcttc acagatgatc ttgttgagcg gattgtggta
    83821 cttgtcgatt tcctcttccg gcttacagtc aacgacgttg taatcgtaga cgaagtacat
    83881 gttggtgccg ttctgatagc tatgagagga atgaccgccc agcatggtga tgtcgtcggc
    83941 gtgcgggaac tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct
    84001 ccagcagccg gacacttcag tggtaaagcc catgttgccg gttttgagga tctgcacacg
    84061 ttcggcagcc actttatccg gtccccagtt caggttgtta aaccaggttt cgatcagctt
    84121 gctgtccacg cgctggcatt gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt
    84181 cgccttcgca atgcgagggt taccttcagc cataaagatc agcacgcatt ttccgtctgc
    84241 aaaatgggta aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg
    84301 atacccttcc accatgactt cacgcaggat gttgaagccg gttttcatgt cttccaggat
    84361 atagccgtag aagaggttgt tttccggggt gaatttaaag attttcactg ttacttcagt
    84421 gatatagcaa agtgcacctt cgttaccgat gatgatgtgg cgaatgtccg ggccagccgc
    84481 gcggcgtggc acgtttttaa tgcgtgtgac ggtgccatca gccaatactg cttccagacc
    84541 aacgaccata tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc
    84601 taccaggccg cccatctgag ccagcggctt tgactgcgga gaatgccccg tggtgtaacc
    84661 tttttcacgc aacgcatttt ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg
    84721 catattctca atatcaatat taatgatttg attcatggcg gagccgtcga gcaccaccga
    84781 gttttccaca acggtttcca gcccaccttc agtggcggaa gcgccggtac gcggcacacc
    84841 gttaattttg tgcgcattca taaaattcag cacacgggag acttgttctg tggaaccgag
    84901 ttttacgacc gctgccggaa tcggcaaagt ataaatacca tgaatatccg gaaatttacg
    84961 aaaacggtca atactgttct tctttaatac tgtttcatcg gtaattacgc gatctgcacc
    85021 aacaatttcc tttagctggt cgacaatcgc tgcgcgagat aaagacatag taattccttc
    85081 ctgataataa aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta
    85141 gcgcactaaa taaccaccat cgaccactaa taaatgccca ttgacataat ttgaagccgg
    85201 acttgcgagg aatacagctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg
    85261 aatatgatca agaacgcgct gattggtttc tggattactg cgtgtcgcca gcgtaatatc
    85321 tgttgcataa tagccagggg cgataccatt tacctgaata ttatattgac ctagctcatc
    85381 acaataagct ttggtgaacc cggcaagagc atgtttagtg gcagaatatg caggtgacca
    85441 ttgtccacct aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg
    85501 gatcataatt tttgcaactt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat
    85561 catcggatcc cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc
    85621 attgtttacc agaatatcaa ctgtaccgaa acgctcacag caagcagcga taatcttctg
    85681 cggcgcgcct tctgcggtga tatccacctg catgaagtcc acctcaacac cctgtttttc
    85741 aatcatttcc tttgtttcgc cgttatcttt gacgaaacta ggaataaaga tatttgcgcc
    85801 agctttggcc aacgccatgg caaatgcctg gcctaaaccg ctattcccac cggtaacaat
    85861 tgcggtttta cctttcagtg agaaaaaatc cattgagaac gcattgagag attcgattga
    85921 catagttagc tccaatttcc gtaaggcaaa aaaaaagaaa ggtaacttcc cccgcgagag
    85981 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    86041 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    86101 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    86161 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    86221 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    86281 ataattagtt ttagcggcgg cgttagtttc gacctggctt atttacgtta tatttatcaa
    86341 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    86401 tttggtattg cggctattat tctttatgcc cccagcggcg ttattgccga taaattttca
    86461 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    86521 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    86581 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc tgggcgatca tagtgagcaa
    86641 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    86701 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    86761 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    86821 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    86881 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    86941 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    87001 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    87061 ggcccacttg gcggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatc
    87121 caaatccttt ccgtactcgg cctgctggcg ttaactgccc tgctcgtcac gaactctaac
    87181 ccgcaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    87241 tcacgcgggc tgtactgggc ctgccctggc gaagcgagaa caccgtctta cattatgggc
    87301 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    87361 atcggccact ggcaagacac cctgcccgca gcagaagcct accgcaatat gtggctgatg
    87421 ggaatggcgg cgcttgccat ggtgattgtc tttacctttt tgctgttcca aaaaattcgt
    87481 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    87541 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    87601 tgatgtacga tctggaaggt aacgtggttt gcgaaggcaa aggcttatta cagccgatgc
    87661 acacgccaga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    87721 ttgccggtca cgatttgatg agtcagtttg ccgggaataa agaagatatt gtcggtattg
    87781 gtctgggatc catccgttgc tgtcgtgcgt tattgaaagc cgatggcacg cctgctgcgc
    87841 cgttgattag ctggcaggat gcacgcgtta cacgccctta cgaacacact aatcctgacg
    87901 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
    87961 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg acatggagcg
    88021 aagatgctgc ggtaatggat aagtttaata tcccccgtca tatgctgttt gatgtgcaaa
    88081 tgcctggcac cgtcctcgga catatcacac cacaagccgc actggcgaca catttcccgg
    88141 caggactgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
    88201 tactggatga tgaaactgcg gtaatttctc ttggcactta catcgcattg atgatgaacg
    88261 gcaaagcact gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    88321 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgtg
    88381 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    88441 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    88501 actggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    88561 gcatggatta cgcatggata tatcgttcga tattggaaag cgtggcgcta acgctgaaga
    88621 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
    88681 gcggcggttc gaacagcgat ctgtttatgc aaatttttgc cgacgtgttc aaccttccgg
    88741 cacgccgtaa cgccattaac ggttgtgcaa gtctgggagc agcgattaat acagcggtag
    88801 gtctggggct atacccggat tacgcaacgg ctgttgataa gatggttcgc gtgaaagata
    88861 tctttatacc gattgagagc aatgccaaac gctacgacgc gatgaataaa ggcattttca
    88921 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    88981 aattggggaa tgtggattcg atccagagct ggtcgaatgc gtaagtgagg aaggccgggc
    89041 gggaaactgc ccggcctgaa catacctgaa tggttatccc cgctgacgcg gggaacataa
    89101 gttcgacaac ttaaaaaatg ataacaagtc cggtttatcc ccgctgatgc agggaacata
    89161 ataaaaactt taactagttc ggtttatccc tgtttttaca aggaatatta actacactcg
    89221 atgtctttag aataaaagac aatacgaatc gcactttatc aattttcact taaagtatga
    89281 actggatatc tttattcata agaactattc atcaacagcg tatagaggcg gttatgtaaa
    89341 accactcatt agcctcaaaa caaagacaaa cttccagatt tattacttaa ccaatgaata
    89401 acaagcttaa aaaacatact gttattctaa agaaaaaatt aacagacatt aattcaatat
    89461 taaaaaaaat aatagattaa aatttcttaa cgatttaaga atcatacaaa taacactttg
    89521 attaaattta attttttgta tcgataattg tgaacctctc tggcatggag aactattttg
    89581 aacatgaggt gttacgtgga tatgttgctt attacaagta ctgctaatat aaaaacttga
    89641 gaaagagata acgggttata tggtggttta tccccgctgg cgcggggaac tcgcctgaca
    89701 ttgcagacgt tacaaattga gaggcggttt atccccgctg gcgcggggaa ctctaatcac
    89761 gttttagcgc gccctcgtcc ggtttcggtt tatccccgct ggcgcgggga actctgtaaa
    89821 cataacctat tattaattaa tgatttttta agccagtcac aatctaccaa ctttatagta
    89881 tcacacaaac aacacatcca ttatgttaaa gagcacttaa tccattgatt aaaaaggtaa
    89941 atatttaaaa taactctata caaactaaaa tctaccaaac tttaccgcaa taattttcac
    90001 tccagcgaaa aattaatgcc acagaatttg tagagaatgc taatgattag ccctggatgg
    90061 gtaaaatgga ggagttttca gaggcgacaa acaatataaa tgagtagaag tctttacagg
    90121 ttccacccat ttataacgct tataaatgtt taatcaggca atatttagat atttatgtgt
    90181 ttgcatcgac aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc
    90241 atccttttgg ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc
    90301 cagtagttca tccagtgctt caatatcacg tacgcgcccc actggatgct tgatttcgtt
    90361 ggctcgctcc agtgcctgtg acaacacgtc atagccgccg cgcatgttca gctttggcga
    90421 tacggtaacc caggtattcg gtgtgcagcg tacttcatga gttccactgg tttcgatctg
    90481 gcagctaaaa ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg
    90541 ctcaccaccc gtaatcacca catgccgcgc ggtatatccc tgccgaccaa tgacagccag
    90601 caaatcttca ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct
    90661 gaaaagggag acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc
    90721 acagccaacc gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc
    90781 ctcaccttgc agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa
    90841 gtggataatt gttaattatt gcagatcctg ccgcaacaat catgtcttat taacattctg
    90901 ttacaggcag atttaaagaa aaatgcgaaa catatcgtta ataattaaag ggagtaacgt
    90961 attatgtcag aagaaaataa agaaaatgga tttaatcatg tcaaaacatt caccaaaatt
    91021 atatttattt tttctgtatt ggtttttaat gataacgaat ctaaaattac cgatgccgcc
    91081 gtcaatttat ttatccagat ttaatgagaa atttataatg cgctatttca tactaatgtt
    91141 cactttcgta tgttcctttg ttgcagccca accaactatt gtcccacaat tacagtaaca
    91201 agttacggat cttacgagta gcttaaactc acaagaaaag aaagaactga cgcacaagtt
    91261 agaatctatt ttcaataata cccaagtgca aattgctgta ttaattgttc ccacaaccaa
    91321 agacgagaca attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc
    91381 caaacgtaat gatgggatac tgatcattgt tgcctggtca gatcgtactg tccgcatcca
    91441 ggtaggttat gggctggaag aaaaggtaac cgatgctctg gctagagata tcatccgtag
    91501 caacatgata cccgcattta aacaacaaaa atttgctcag ggattagagc tagctataaa
    91561 cgctttgaat aaccaactca cttcacaaca ccaatatccg actaatcctt cagaaagtga
    91621 atcagcgtct tccagtgatc attattactt tgctattttt tgggtatttg cagtgatgtt
    91681 cttccctttc tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagtggagt
    91741 ctatatttca gcaatctatc ttttagattt attcctgttc tccgataaaa ttttttccat
    91801 tgctgtattt tccttctttt ttactttcac catatttatg gtctttacct gtttatgtgt
    91861 acttcagaaa agagcatcag gtaaaagtta tcattcagac aacagcggtt ccggtggagg
    91921 ctcaagtggc ggcggattta gcggcggtgg cggttcttct ggaggtggcg gcgcatccgg
    91981 ccgctggtaa ctcaaccata aaaaatgcca gcccggaggc tggcattttt aaatcagata
    92041 aagtcagtct tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
    92101 cagagcttct tcgatacgaa tcagctggtt gtatttagca acgcggtcag aacggctcat
    92161 agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc
    92221 ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcatctt tcgccatctt
    92281 gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt
    92341 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
    92401 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc
    92461 agattcgtcc agaccgtctt cgatagaaac gatcgggtac tgtttggtca gttcttccag
    92521 gaagtgagtg aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt
    92581 accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
    92641 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
    92701 caggttcggc gcatagccac cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag
    92761 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
    92821 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
    92881 accgttgatg atgttcatca tcggaaccgg catagagtat ttgcccggag taccgttcag
    92941 ttcagcgatg tgctcgtaca gcggcatccc tttagcagct gcagcagctt tggcgttagc
    93001 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
    93061 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
    93121 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
    93181 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
    93241 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
    93301 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
    93361 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg
    93421 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
    93481 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
    93541 agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga
    93601 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
    93661 gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct
    93721 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gttccgccga gatcgctctt
    93781 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
    93841 cgggtactta cattctggca caaattccgt agagttggcg ttctccatgt tggcaacatg
    93901 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
    93961 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccaaaacc
    94021 gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg tttcaacatc
    94081 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
    94141 tgcttcgatc actgatttat aagcatccgg cagttcaatg tacttgccga ccataccgat
    94201 ggtgacttca cttaccggat tcgcttcttc gaagataacc tgttcccatt cggacagatt
    94261 cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
    94321 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
    94381 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacag cgcgatctga
    94441 acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg
    94501 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
    94561 aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg
    94621 caaggattcg atatcaccta ctgtaccgcc gatttctacc agtactacgt catgaccttc
    94681 gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgcacggt
    94741 tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt
    94801 ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc
    94861 caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
    94921 agtacctgga tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg
    94981 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
    95041 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
    95101 gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact
    95161 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
    95221 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg
    95281 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
    95341 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
    95401 tccacttcgc gaaaacgacg ctcaaatttt tcgttcgctt tttgcaatgc gatttccgct
    95461 tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcccc catttcctcc
    95521 tccagtttag cctggtcgac aacagcctgc cgcgcttcgt acatcacctc gtcgatctct
    95581 tcgtagactt tatcgactac cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa
    95641 cgtttctgga ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc
    95701 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
    95761 acttcactac tgttttcggc agaactatca gcaaaaacat gcggatggcg acgctctaat
    95821 ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
    95881 tgcgcgtaaa acaccacctg gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa
    95941 tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg
    96001 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc
    96061 ataatagtga gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt
    96121 caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa tcagtacgtt
    96181 aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct tcgttgctcc
    96241 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
    96301 accggataaa acaacttcga acggatatcc ttttgattgc gttgtataag gaacacacag
    96361 acacatacct gttttgttgt tgtacatgaa aggactcagg acaacagctg gacgatgtcc
    96421 agcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
    96481 tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattt
    96541 tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct
    96601 ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca
    96661 atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg tgaatttccc
    96721 caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca tatgtagata
    96781 cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc
    96841 gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcgc cccagcactt
    96901 gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac
    96961 ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta
    97021 acaacccact acgatcatta gccaccacac ggaccaccag cgaatatccg gcggagtagc
    97081 tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt
    97141 gttcgcaatc ggcgcggtgt actgaaatac cgcgcccctg ggtaatgaag ccgacaatct
    97201 catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggttg ccaacacctt
    97261 cgaccaccac gcgaccgtta tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa
    97321 gttgcttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat tgcgattgca
    97381 ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc agcaactcgt
    97441 cgacatcatt aaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc
    97501 ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt
    97561 cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga
    97621 ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct
    97681 ggtcgcccat ctgcagctgg taggtgaacg gcacaatgcg cccgccaatt tttgccccga
    97741 tgcagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg
    97801 caggcaaatc aacgacatca cctttcggcg taaagacata cacccggtcg tcaaagacct
    97861 ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc cacgcaatca
    97921 gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc
    97981 cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttgg
    98041 tgcggatttg gatctcaacg gttttgccac ctggccccag aaccacggta tgaatagact
    98101 gataaccgtt tggtttcggg ttagcgacat aatcgtcaaa ctcatccggc aggtggcgat
    98161 agtgagtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg gcgacaatac
    98221 gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc attttgcgcc
    98281 agatactgta gatgtgtttc ggacgaccat acacttcagc tttaacgcct tcagctttca
    98341 tctcagcgcg cagatggccg acaaactctt cgatatagtg ttcgcggtcg agacggcgtt
    98401 catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg aagcagtaat
    98461 cttccagctc ccatttcagt tgcccaatcc caagacggtt cgccaacggc gcgtagatat
    98521 tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt acttcgcgca
    98581 gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaaatcatcg accatcgcca
    98641 ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt
    98701 tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac
    98761 cgacgctctc acgcagcaca tcttcgctga ctacgttggc atcagccaga gggaacagca
    98821 gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa
    98881 caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg
    98941 cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc
    99001 atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccattg
    99061 tcctctcctt tagggaccag acctgccgaa atcggcaaat cgtaactatt taacgcgtta
    99121 acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat atccagcatc
    99181 gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg agccagcgtt
    99241 gcagggttac aggatacata aactatacga ataggttcca gttttataat ttgctgcata
    99301 acacctgcgg cacctgctcg cgccgggtcc agcaacactt tatcgaagcc gtttttcgcc
    99361 cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac attctgtaaa
    99421 ccattaagac gcgcattctg ctggcctttt tccaccagcg ccggaacacc ttctacaccg
    99481 accacactgg ctgcttgttt cgccaatggc agtgtaaagt tgcccatacc gcagaacaga
    99541 tccagtacgc gatcttcagg ttgcacatcc agccattcca acgcacgcgc taccattttt
    99601 tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa gcgcaacccg
    99661 tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact atcgggggcg
    99721 agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg atctgccgaa
    99781 cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg taccagttca
    99841 acatgaccaa gatggcgcat aacttgtaag ctgcccaggc atgccctgac tttgggcagc
    99901 aatgcttcaa gttggggcac taaaatgggg cattgtttaa cgtcgacaat gtcactggag
    99961 cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt cagacttaaa
   100021 cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca cttcagagac atcgtgtttc
   100081 attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct ggcgtgttgt
   100141 tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gtgtttcgcg ttccgggcta
   100201 tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt ttttatcttc agtaacagca
   100261 acttccgcgt tttcctgcgg caataatccg gggataaata acgttttgcc gttatgtcgc
   100321 gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat ctgacgcgtc
   100381 gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc aatttaacag
   100441 tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc gcgcacgcat
   100501 gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta tctttttcgt
   100561 cgtgcatcgc tataacgact tgcagcgtca actggaagat gccggtgcca gcattattga
   100621 gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat ctatcggtca
   100681 gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt cggtttatga
   100741 tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat caatgcagct
   100801 cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg atattatgat
   100861 cctccgcacg ccgattattt ctgagagtta ctcccccgac gaatcgccca gtagcgatgc
   100921 caaaaatagt caaaatatgt tgggatatat tgcgctggag ctggatctta aatcggttcg
   100981 cttgcagcaa tataaagaga tctttatttc cagcgtgatg atgctgtttt gtatcggtat
   101041 tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt
   101101 gaataccgtc gaccgcatcc gtcgcgggca actcgacagc cgggtggaag gatttatgct
   101161 cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc tggctgctta
   101221 tcacgaagag atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg aaacgctgga
   101281 gcagatggaa attcagaacg ttgagttaga tctggcgaaa aagcgcgccc aggaagcggc
   101341 gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac cactgaatgg
   101401 tgttattggc tttacccgcc tgacgctgaa aacagaatta acaccaacgc agcgcgatca
   101461 cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg atgttctcga
   101521 cttctcgaaa ctggaagcag gtaagctgat tctggaaagt attccattcc cactacgcag
   101581 cacgctggat gaagtcgtta ctctgctggc acattcttct cacgataaag ggttggaact
   101641 gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac tgcgattaca
   101701 gcaaatcatc actaacctgg tggggaatgc aattaaattc accgagaatg gcaacattga
   101761 tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag tgcagattcg
   101821 ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg ccttccgaca
   101881 ggctgatgcc agtatttccc gccgtcatgg tggcaccggt ctggggctgg tgattacaca
   101941 aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agccagccga atcgcggttc
   102001 aactttctgg ttccacatta atctcgatct gaacccgaac attattatcg aagggccatc
   102061 cacccagtgc ctcgccggta agcgcctggc ctatgtcgaa ccaaactccg cagcagcgca
   102121 atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc caacgttctc
   102181 cgcgctgcct cccgcgcatt acgacatgat gttgttaggc atcgcggtga ccttccgcga
   102241 gccgctaaca atgcaacatg agcgattagc gaaagcggta tcgatgaccg atttcctgat
   102301 gctggcactt ccttgccatg cacaagtcaa tgcagaaaaa ctcaagcaag atggtatcgg
   102361 cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga cggaattttg
   102421 tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agcaagctgg caatgacagt
   102481 catggcagtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc tggaagatat
   102541 ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat
   102601 gccgttcgat ttgatcttaa tggatattca aatgcctgac atggatggca ttcgggcctg
   102661 cgagctcatc caccagctcc cccatcagca acaaacgccg gttatcgcgg taacggcgca
   102721 tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg atgagcgatt atctggcgaa
   102781 accgattgaa gaagagcgat tgcataattt gttgttgcgc tacaagcctg gcagcggtat
   102841 ttcctctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga atgcgaccct
   102901 cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc gcgatatgct
   102961 gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg
   103021 agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt gcggctatag
   103081 cggtgtgccg cgtatgaaga atctctgcca actgatcgaa caacagctac gtagcggtac
   103141 taaagaagaa gatttggagc cggagctgct ggaactgttg gacgagatgg ataatgtagc
   103201 gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt cgagtagcaa tcggcttttt
   103261 atgacgctgg tttcagattg gtgacaaagt gcctgtgttt atgccggatg cggcgttaac
   103321 gccttatccg gcctacaaaa ccaattaaat tcaatgaatt aaaaaatgat gtagacctga
   103381 tacatagcac aacaggctat tttgcgttta gcatcagtct caagccggct ccagatagag
   103441 ccggttttgg ttttctgtct taacgcacca tgcacgggcg cttgttatcg aacgtccagc
   103501 caggaatcag atactgcatt cccatcgcat cgtcacgcgc gccaagcccg tgtttctgat
   103561 acagctcatg ggctttcatc acttgatcca tatcgatttc tacacccagc cccggttttt
   103621 ctggcacctg taccagcccg cctttgatct caaacggttc tttggtcagg cgctgattac
   103681 cttcctgcca gatccagtgc gtatcaatag cagtaatttt acccggtgca gcggcggcaa
   103741 catgggtaaa catcgccagg gaaatatcga agtggttgtt tgagtgtgaa ccccaggtca
   103801 ggccaaattc atggcacatt tgcgccacac gtaccgaacc ttgcatcgtc cagaaatgcg
   103861 gatccgccag cgggatatca acggattgca gggagagcgt atggcccatc tgccgccagt
   103921 cggtggcgat catattggtt gcggtcggca ggccagttgc gcgacggaac tctgccatca
   103981 cttcacgccc ggagaaacct tgctccgcac cacacggatc ttctgcataa gccagcgacc
   104041 ctttcaggta tttaccaatt ttaatcgctt cgttcagcga ccaggcaccg ttaggatcga
   104101 gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac aatagactcg gcctcttctt
   104161 ccccggccag tacaccgcct ttcagtttga aatcgttgaa gccatatttt tcatacgccg
   104221 cttccgccag gcgcaccacc gcatccggcg tcatcgcttc ttcatgacgc aggcgatacc
   104281 agtcgcatga gtcatccggc tggctttgat acggcagcgg tgtggctttg cgatcaccga
   104341 cgaagaacag ataaccgagc atttcgactt cgctacgctg ttgaccatcg cccagcaggg
   104401 atgccacgtt tacccccaga tgctgcccca gcagatccag cattgccgct tctatcccgg
   104461 taactacatg aatagtggta cgcaaatcaa atgtctgcaa accgcgccca ccagcatcac
   104521 gatcggcaaa agtattacgc accagcgtca gaacgttttt gtattcaccc agcgttttac
   104581 ctaccaccag cggaatcgca tcttccagcg ttttacggat tttctcgccg ccgggaattt
   104641 cccctacgcc agtgtgacca gaattatctt tgataatcac aatattacgc gtaaagaacg
   104701 gtgcgtgtgc accactcaga ttcatcagca tactgtcatg acccgccacc gggatgacct
   104761 gcatttcagt aacaacaggc gtcgtaaatt gagaactcat atttatgtcc tttttcagaa
   104821 tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc cagccgggga tcaggtactg
   104881 catcggacct gcgtcgttac gcgcaccgac aggcagacgt ttataggcct catgtgcctt
   104941 ttgtacctgt tcccagtcca gttccacgcc cagaccgggc gcatcaggaa cggcaatttt
   105001 tccgttttta atctccagcg gatttttggt caggcgacaa tcgccctcct gccaaatcca
   105061 gtgggtatcg atagcggtag gattacccgg tgccgccgcg cccacatggg taaacatcgc
   105121 cagagagata tcgaaatggt tattagaatg gcagccccag gtcagccccc agtcgtcgca
   105181 aagctgcgcc acacggactg caccggaaag cgtccagaag tgcggatcgg caagtggaat
   105241 atctaccgca ttgagcatca ccgcatgacc catttcgcgc cagttggtgg cgatcatgtt
   105301 agtcgcgacg ggcaagccgg tcgcccgccg aaactccgcc ataacttcac gaccggagaa
   105361 accctgttct gcgccgcatg gatcttcggc ataggtaaga acatcattca gccctttgca
   105421 tagagaaatg gcttcatcaa gtagccaggc accgttggga tcaacggtaa tccgcgcatc
   105481 agggaagcgt ttcttcaatg cacgaacagt gtcgatttct tgctcgccag gtaacacccc
   105541 gcccttaagt ttgaaatctt taaagccata gcgatcctgt gaggcttccg ccagacgcac
   105601 aacggcttcg ctgttcatcg ctttctgatg gcgcaactga taccactcat ggttgcccgg
   105661 cgtattttcc agataaggaa gatcggtttt ggtccgatca ccgatataaa acagataacc
   105721 gaggacggta atagtctcgc gttgcttgcc tggccccaac agttcgcaga ccggaacatt
   105781 cagcgcctta cctagcaggt caagcaaggc ggcttccagc gccgccacgg cattaacgcg
   105841 caattcaaaa gtccaggcac ctttgccgaa ggtatcaaaa tcggctgcct gattaccttt
   105901 atgcacctgc tgaaccactt tattcaggcg cgcaacttcc tggcccagaa ccatcggaat
   105961 agcatcgacc agtgtttgat aaatcacctc tccgcccggc gcttcaccaa tgccggtatg
   106021 cccggcgtta tcggtgagta ccacaatatt gcgggtgaaa tatgcgttat gtgccccacc
   106081 aatattaagc aacatgctat cgtgcccggc gaccggaatg actttcatat cagtaataac
   106141 aggactggat tgtgtcgtca tcttattgcc ccgcgacagg tttcagctcg atacgcttga
   106201 tatcgcccac cagcaccagg tagctcagta ccgcgattaa ggcatgaaca ccaacataaa
   106261 tcagcgcccc attaaaggag ccagtcgtgc caacgatata accaattgcg attggcgtga
   106321 cgatgccaga aatgttaccg aacatgttga acaggccacc ggaaagaccg ctgatctctt
   106381 ttggcgcggt atctgccatt actgcccaac ccagcgcccc gatgccctta ccgaagaagg
   106441 ccagcgccat aaagccgatg atcatccact caacgttgac gtagttgcag aacaccatca
   106501 ccatcgacag caacatgccc attacgatcg gtgttttacg cgcaatgttc agcgatcccg
   106561 tgcggcgcat cagccagtcg gaaataatcc cacccagcac accgccgata aaaccgcaaa
   106621 ccgccggaac ggaagccaca aagcccgctt tcagaatcga catcccacgc gcctgcacca
   106681 gataaaccgg aaaccaggta atgaaaaagt aagtcagggc gttgatacag tactgaccga
   106741 tataaacgcc gatcatcatc cgtgacccga gcagttgttt gatctgcccc cacttcacgc
   106801 tgaacggcac tttaactttg gtgttttgct gatccatatt gatcagcgcg ccacccgcgg
   106861 cgatgtactc cagctctttc tgatttaccc ccggatgttg atttggctcg tggatgactt
   106921 tcaaccagat aaagctgatg acaatcccca gaccgcccat aaagaagaag acgtgtgacc
   106981 agcccacttc atgcgtcagc cagcccataa tcggcgcgaa gatcaccgtt gcgaagtatt
   107041 gagcggagtt aaaaatcgac accgccgttc ccctttcctg cgccggaaac caggccgcaa
   107101 caatgcgact gttgccgggg aaagatggcg cttcagcaag cccgaccagg aagcgcagag
   107161 taaacagggc aacgataatg ccgaatccac taaagatatc gacgaagcct tgcagcaagg
   107221 taaacatcga ccagataaag atcgaccaga agtagacgcg ttttgaacca aaacgatcca
   107281 gcaaccaacc accagggatc tgcccgataa cataagccca tgagaaggca gagaacacat
   107341 agcccattcc cacgggatca aggccgatat ctttggccat ttccgaaccg gcaatagaaa
   107401 gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa caacatcacc actatccagt
   107461 aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg acttaaagaa ctcattgttg
   107521 cactcctgaa aattcgcgtt agccacgctc actctggact gcgacatagc caggaaatca
   107581 gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt tcgccgaata cggcgcgtgt
   107641 ttatatctgg caatagcagt ataaaaagcg cgccatagcg gctcaccgtg caacaacaca
   107701 acattaatgc gttcaatgag gcccgatttt ggcattagcc ctggacggtg gaatccactt
   107761 cacggaaatg aaaacaagaa caagaaagga agggttaaaa cgaagaaata aagaagagta
   107821 tgaaatggat cgcttgactc caggcaaacg ccagtaaaaa tccgcgctat gaagcagttt
   107881 ttactggcat ttgcctgaaa atattcgatt cagcaccgct aaaacgacat ttaccgctcg
   107941 ctgaacatat caggacaaca gcgtgcccca ctgttcgacc cacggatttg attccgtttc
   108001 cggttccggg ttctcactgg cgtcaatcaa cagcatttcg ccaacccgct gagcgctctg
   108061 ttcctgcaac aaggcatcga actgtttgcc gccattgcag aaatttacat aactgctgtc
   108121 gccgagcgca atcacgccat aacgcagatt cggctggaag cccagactat ctttgattcc
   108181 ctgaaagagc ggcacaatgc tatcaggaag gtcgccctgc ccggtcgtgg acgtaaccac
   108241 cagaacatac ttatcctgat agggcagcca gtcgcttaat tcaggatctt caaataccgt
   108301 tgctttgtgg ccctgtgcgg tcagaattgc ttctgcttct tccgccacta acagcgaatt
   108361 tccgtacatg gtgccgacaa aaataccaat ttccgccata ccgttttctc cctggattag
   108421 gaacttatct ctccatcctg acccgatggc gcactgaact caaccctttc attttcagga
   108481 agcagaccgc gccagccaaa ttgtgataac gcctgcatcc aggtgtcgtc caggcccgcg
   108541 tgaatagtca gcggctcgcc agtaaaagga tgcgtcaatg acaactgact ggcatgtagc
   108601 attaaccgct ggaggccaaa atgctcagca ccgctgcgat tctggcgtaa atcgccatgt
   108661 ttgctatcgc caataatcgg atgacgcaaa tgggcaagat gtcggcgaag ctgatgtttg
   108721 cgtccggttt tcggctccag ttccaccagg ccgtagcgcg tggtcgggta acgtccggtc
   108781 gctaccggca tttctacggt cgccagaccg cgataatgcg tcactgctgg ctgcgagcct
   108841 ttatcttcgc gggcaaattt atcagcgatt ttgtccagtt cttccaccag tggataatcc
   108901 agaactgctt cttccatcaa ccagccgcgc acaatcgcat ggtaacgttt ctggatttgg
   108961 tgctgttcaa actgttgtgc cagcagccgt ccggcctcgc tggataatcc catcaacaac
   109021 acaccagaag tgggtcggtc cagacgatga gcggtaaaaa cgtgctggcc aatctgatca
   109081 cgcacggttt gcatgaccac tactttctcg tcgcgatcca gccagctgcg gtgaaccagc
   109141 cagccggagg gtttatttac cgcaaccagc cattcatcct gatagagtat ttccagcatt
   109201 agctcgcatc atccgcaaaa agagcatcca gtttttccag ctcagccaga ataagcgcgc
   109261 gttgcggatg gtccgtcgcc agcgccattt cataataggg tgcaacggca aaagcgcccg
   109321 gtaacggctg tttgttatct aacaaatcgt gcattcgcgg gatcagcacc cactgcaacc
   109381 actccagtgg ttccatggtg tccataaaga acggttgggt actattaaat tgatgcggct
   109441 ggggttcatc gtttcgccag tgctgatgtt cacgcagtaa cgcttcaagc gcctgcaact
   109501 ggagacgaac gcggtcatga gtggtcataa agcaacctca ataagaaaaa ctgccgcgaa
   109561 ggatagcagg aataaaaaaa gggagcactg tattcacagc gctcccggtt cgtttcgcag
   109621 cattccagct acttttgttg ctccctgctc atccttgaca acttttcctc tggccttgcg
   109681 gccaatcgtt catcctgaac tattgcttcc tgctcacacc accccgatgt gatacttcat
   109741 cctgaagtgt ccctggcctt cctgacccac cgaatcatcc tgaccggttc tcattctcca
   109801 tcctggaggt gtcctttaac gcgtcctgcg tcatcctctt cgcttcatcc agaagccttt
   109861 ccctgaaaca ccatcctggt gtgtcctgca gaagtgtcat catcctgatg ttcacttcgt
   109921 tgtctgactc cctgtcgacg aagatagaat cgtctttttc gcgaagtctt acaaggcgct
   109981 tacagacaat gccttaagga aaatttcata tgaaagttaa gaattaacaa tcaacatttt
   110041 gattttaaat gaaaataacg attcttatct ttttaagtat tcgcttattt cctggcgatc
   110101 tcctacagag tctgtaagag atctctcaca accagatggg gtatttggat tacagaagag
   110161 gcttaagttg attaaggaat tccgcaagat ttgaggctaa atgcgtccgt ttacgtgttc
   110221 ccagcgtctc tttgcacact tcaccggaga gattacacac cgagatgacc tctaactctt
   110281 cttccagcgt cgcgataaat agcgtgggcg gcagttttag ccttttctga gtcaccagat
   110341 gaccaatcag attttcctgc acccgacgga agtcgtcttc actccaggtt tgcagcaatg
   110401 tcagtttgat atcgccaaac tgcgcgtgca tatccccggc aaactgagtc gtataaaagg
   110461 tatgaattgt gggttgtatc acaatatcaa aagcgcgttc aaccgcgttt acattttgtt
   110521 ccccggtaaa cggctggggt tgccagtata cggcatcttc ggtagttgaa ataatgcacg
   110581 gtgaaggaac accgtacagt tcctcactta acggccagct tttatgctct tcatgccacg
   110641 catcgcagta gcgtgcagta aaatctttca gggcttgtgc ggtcaaatcg tccactaatt
   110701 tctctcttca cttaaaccag atacacttgt cgtttagttt atctggttta tgacggtgaa
   110761 acatgtcttc ttatgcaaac catcaggcac ttgcgggcct gactcttgga aaatcaaccg
   110821 attaccggga tacctatgac gccagcctac tgcaaggcgt tccacgcagc ctgaatcgcg
   110881 acccgctggg tctgaaagcg gataacctgc cttttcacgg tacggatatc tggacgctgt
   110941 atgaactttc ctggctgaat gcgaaaggtt tgccgcaggt cgctgtcggt catgttgaac
   111001 ttgattacac cagcgtaaat ctgattgagt cgaagagttt taagctctat ctcaacagtt
   111061 ttaaccagac gcgttttaat aactgggatg aggtgcgcca gacgctggag cgcgacttaa
   111121 gcacttgcgc ccagggtaag gttagcgtgg cgttatatcg tcttgatgaa ctggaaggcc
   111181 agccgatagg tcatttcaat ggcacctgca ttgatgacca ggatatcact atcgataact
   111241 atgaattcac tactgactat ctggagaatg ccaccagcgg tgaaaaagta gtggaagaga
   111301 cgcttgtcag ccacctgctg aaatcaaact gcctgatcac ccatcaacca gattggggtt
   111361 cgatccaaat tcagtatcgt ggacgccaaa ttgacagaga aaaactgctg cgttacctgg
   111421 tctcattccg tcatcacaac gagttccacg aacagtgcgt ggaacgcatc tttaatgacc
   111481 tgttacgctt ctgccagcca gaaaaattga gcgtttacgc acgttatacc cgtcgtggcg
   111541 gtctggacat taacccgtgg cgcagtaata gcgattttgt cccatcgacc acaagactgg
   111601 ttcggcaata aattttttct caattttgcg tgctggattc acgcagaagg ttgtgaaagg
   111661 tcatcaggca gggctattgt aatcaaaggg aatgacgata ttcgtcccat aaggagtttt
   111721 tcttgattac acatattagc ccgcttggct ccatggatat gttgtcgcaa ctggaagtgg
   111781 atatgcttaa acgcaccgcc agcagcgacc tctatcaact gtttcgcaac tgttcacttg
   111841 ccgtactgaa ctccggtagt ttgaccgata acagcaaaga attgctgtct cgttttgaaa
   111901 atttcgatat taacgtcttg cgccgtgaac gcggcgtaaa gctggaactg attaatcccc
   111961 cggaagaggc ttttgtcgat gggcgaatta ttcgcgcttt gcaggccaac ttgttcgcgg
   112021 tcctgcgtga cattctcttc gtttacgggc aaatccataa caccgttcgt tttcccaacc
   112081 tgaatctcga caactccgtc cacatcacta acctggtctt ttccatcttg cgtaacgctc
   112141 gcgcgctgca tgtgggtgaa gcgccaaata tggtggtctg ctggggcggt cactcaatta
   112201 acgaaaatga gtatttgtat gcccgtcgcg tcggaaacca gctgggcctg cgtgagctga
   112261 atatctgcac cggctgtggt ccgggagcga tggaagcgcc gatgaaaggt gctgcggtcg
   112321 gacacgcgca gcagcgttac aaagacagtc gttttattgg tatgacagag ccgtcgatta
   112381 tcgccgctga accgcctaac ccgctggtca acgaattgat catcatgccg gatatcgaaa
   112441 aacgtctgga agcgtttgtc cgtatcgctc acggcatcat tatcttccct ggcggtgtgg
   112501 gtacggcaga agagttgctg tatttgctgg gaattttaat gaacccggcc aacaaagatc
   112561 aggttttacc attgatcctc accggcccga aagagagcgc cgactacttc cgcgtactgg
   112621 acgagtttgt cgtacatacg ctgggcgaaa acgcgcgccg ccattaccgc atcatcattg
   112681 atgacgccgc tgaagtcgct cgtcagatga aaaaatcgat gccgctggtg aaagaaaatc
   112741 gccgcgacac tggtgacgct tacagcttta actggtcaat gcgcattgca ccagatttgc
   112801 aaatgccgtt tgagccgtct cacgagaata tggctaatct gaagctttac ccggatcaac
   112861 ctgttgaagt gctggctgcc gacctgcgcc gtgcgttctc cggtattgtg gcgggtaacg
   112921 taaaagaagt cggtattcgc gccattgaag agtttggtcc ttacaaaatc aacggcgata
   112981 aagagattat gcgtcgtatg gacgacctgc tacagggttt tgttgcccag catcgtatga
   113041 agttgccagg ctcagcctac atcccttgct acgaaatctg cacgtaatct ccgctccccc
   113101 ggtgacgttt gttgccgggt tttctcgttt ttggtcactt actcatcaac tcatttcatt
   113161 tgttatatga atgtttctta ccaccctcac gcgacaaata tcatcacagt taatatgtca
   113221 tacaatttat gttgcaacgc aaacgtttcc ctattttcat aaaccgtatt ttatcgctat
   113281 gaaaaagaaa ttatcgccat gattaactaa aagtattgat tttttcagtt caacctacat
   113341 atattgcgcg ccccggaaga agtcagatgt cgtttaatgg gcaaatattg cccttaaatt
   113401 ctcttttact tttgatttac agagtaaagc gttgggataa tctatcttcc aagtagatta
   113461 ttgtatttga gatcaagatc actgatagat acataacttg tgtgtatctt tccgccctca
   113521 aattattacg gcggtaaatg attaagccat cgccgataga cagatttcat ttttacggtc
   113581 aggcaccttc ccgggctgaa ctggctaaaa gctgaattat ttgcattcct ccaggagaaa
   113641 tagatggaaa cgactcaaac cagcacgatt gcgtcgaaag actctcgtag tgcctggcgc
   113701 aagacagaca ccatgtggat gctgggcctt tacggcacgg caatcggcgc gggcgtgctg
   113761 ttcctgccaa tcaacgccgg tgttggcggt atgatcccgc tgatcatcat ggctatcctt
   113821 gcgttcccga tgacgttttt tgctcaccgc ggcctgactc gcttcgtact gtctggtaaa
   113881 aacccgggcg aagacatcac cgaggttgta gaagaacact ttggtattgg cgcaggtaaa
   113941 ctgattaccc tgctctactt cttcgctatc tacccgatcc tgctggttta tagcgtggca
   114001 atcaccaata cagttgaaag cttcatgtct caccagctgg gtatgacgcc accgccgcgt
   114061 gcgattctgt cgctgatcct gatcgtgggt atgatgacca tcgttcgctt tggtgagcag
   114121 atgatcgtta aagcgatgag tattctggta ttcccgtttg ttggcgtact gatgctgctg
   114181 gctctgtacc tgatcccgca gtggaacggc gcagcactgg aaacgctgtc tctggacact
   114241 gcatctgcaa ccgggaacgg tctgtggatg accctgtggc tggcaattcc ggtaatggtg
   114301 ttctcgttca accactctcc gatcatctct tctttcgccg ttgcgaagcg tgaagagtac
   114361 ggcgatatgg cagaacagaa atgctcgaag atccttgcat tcgcacacat catgatggtg
   114421 ctgaccgtaa tgttcttcgt cttcagctgc gtactgagcc tgactccggc agacctggct
   114481 gcggctaaag agcagaacat ctcgattctg tcttacctgg ctaaccactt taacgcaccg
   114541 gttatcgcgt ggatggctcc gattatcgcg attatcgcta tcaccaaatc cttcctcggc
   114601 cactacctgg gcgcacgtga aggcttcaac ggtatggtga ttaaatctct gcgtggtaaa
   114661 ggtaagtcta tcgaaatcaa caagctgaac cgtatcactg cgctgttcat gctggtaacg
   114721 acctggattg ttgccaccct gaacccgagc atcctgggta tgattgaaac cctgggcggc
   114781 ccaatcatcg cgatgatcct gttcctgatg ccgatgtacg caattcagaa agtaccggca
   114841 atgcgtaagt acagcggtca catcagcaac gtattcgttg tcgtgatggg tctgattgca
   114901 atctccgcaa tcttctactc tctgttcagc taagtccttt cgcgccgctt tcgggcggcg
   114961 cttcctccgt tttaacgcga tgtatttcct atgattagcg tattcgatat tttcaaaatc
   115021 ggcattggcc cttccagttc tcataccgtt ggaccaatga aagcgggtaa acaatttacc
   115081 gacgatctga ttgcccgtaa tctgcttaaa gacgtgaccc gcgtggtggt tgacgtgtac
   115141 ggctcgctct ctctgaccgg taaaggccac cacactgata tcgccattat tatgggcctg
   115201 gcgggtaacc tgccggatac cgtggatatc gattccatcc ctggttttat tcaggatgtg
   115261 aatactcatg gtcgcctgat gctggcaaac ggtcagcatg aagtggagtt cccggttgat
   115321 cagtgcatga acttccatgc agacaacctt tctctgcatg aaaacggtat gcgcattacc
   115381 gcgctggcgg gcgataaagt cgtttacagc cagacttact actctattgg cggtggcttt
   115441 atcgttgatg aagagcattt tggccagcag aatagcgcac cggttgaagt tccttatccg
   115501 tacagttcag cagccgatct gcaaaaacat tgtcaggaaa ccgggctgtc actctctggc
   115561 ctgatgatga aaaatgagct ggcgctgcac agcaaagaag agctggaaca gcacctggcg
   115621 aacgtatggg aagtcatgcg tggcggtatt gagcgcggta tttccaccga aggcgtgttg
   115681 cctggcaaac tgcgcgttcc acgccgtgct gcggcactac gccggatgct ggtcagccag
   115741 gataaaacca ccactgaccc gatggcggtt gttgactgga tcaacatgtt tgcactggca
   115801 gtgaacgaag agaacgctgc tggcggacgc gtggtgactg cgccgactaa cggtgcgtgc
   115861 gggattatcc cggcagtgct ggcgtactac gacaagttta tccgcgaagt gaacgctaac
   115921 tcactggctc gttacctgct ggtagccagt gccattggtt ctctttataa gatgaacgcg
   115981 tcaatttctg gtgctgaagt cggctgccag ggtgaagttg gtgtggcgtg ctcaatggcg
   116041 gcggctggtc tggcagaact gttaggtgca agcccggcgc aggtgtgcat cgcggcggaa
   116101 atcgccatgg agcacaacct cggtctgacg tgtgacccgg tcgccggaca ggtccaggtg
   116161 ccatgcatcg agcgtaacgc cattgcggca gtaaaagcgg tgaacgccgc acgtatggcg
   116221 ctacgccgta ccagcgagcc gcgcgtctgc ctcgataaag ttatcgaaac catgtacgaa
   116281 acaggtaaag atatgaacgc caagtaccgc gaaacctctc gcggcggcct ggcaatgaag
   116341 atcgttgcct gcgattaatc gctctccaga ggcctcgttt tgcgaggcct cttcccgatt
   116401 tctcatccag ccgtagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
   116461 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat tcgtcgcatt
   116521 catgccgttc agggatcgcc ctgtgtcgaa acctgtcagc atgcgctcga tcagctcatt
   116581 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
   116641 tggcgtcatc agcgtttacc agattacaaa gcgggtcgac cgccaatgcc ggaagagttg
   116701 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
   116761 accagcggca acgaagccga tgacttagcc gccacgctgg cggtcaaagt gacacaggcc
   116821 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
   116881 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
   116941 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
   117001 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
   117061 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
   117121 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
   117181 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
   117241 gccgcaaacg tcgtatccgg cattatcaca tcagcgcatt taccaggcgg tatggtaaag
   117301 ctct
//
LOCUS       DAHBHC010000004       109910 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHBHC010000004 DAHBHC010000000
VERSION     DAHBHC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.004"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..192)
                     /locus_tag="M2A35_000340"
     CDS             complement(<1..192)
                     /locus_tag="M2A35_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196688.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDL"
     gene            complement(621..3035)
                     /locus_tag="M2A35_000341"
     CDS             complement(621..3035)
                     /locus_tag="M2A35_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7196689.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLLLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHLELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(3032..3718)
                     /locus_tag="M2A35_000342"
     CDS             complement(3032..3718)
                     /locus_tag="M2A35_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7196690.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLGHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            3689..4312
                     /gene="tesA"
                     /locus_tag="M2A35_000343"
     CDS             3689..4312
                     /gene="tesA"
                     /locus_tag="M2A35_000343"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCB7196691.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            4302..5111
                     /gene="ybbO"
                     /locus_tag="M2A35_000344"
     CDS             4302..5111
                     /gene="ybbO"
                     /locus_tag="M2A35_000344"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCB7196692.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            5172..6026
                     /gene="cnoX"
                     /locus_tag="M2A35_000345"
     CDS             5172..6026
                     /gene="cnoX"
                     /locus_tag="M2A35_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCB7196693.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(6089..6868)
                     /gene="fetB"
                     /locus_tag="M2A35_000346"
     CDS             complement(6089..6868)
                     /gene="fetB"
                     /locus_tag="M2A35_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCB7196694.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(6855..7532)
                     /gene="fetA"
                     /locus_tag="M2A35_000347"
     CDS             complement(6855..7532)
                     /gene="fetA"
                     /locus_tag="M2A35_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCB7196695.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7678..8595
                     /locus_tag="M2A35_000348"
     CDS             7678..8595
                     /locus_tag="M2A35_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCB7196696.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            8592..9050
                     /locus_tag="M2A35_000349"
     CDS             8592..9050
                     /locus_tag="M2A35_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCB7196697.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(9051..9458)
                     /gene="cueR"
                     /locus_tag="M2A35_000350"
     CDS             complement(9051..9458)
                     /gene="cueR"
                     /locus_tag="M2A35_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCB7196698.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(9583..10875)
                     /locus_tag="M2A35_000351"
     CDS             complement(9583..10875)
                     /locus_tag="M2A35_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCB7196699.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(10878..11810)
                     /gene="glsA"
                     /locus_tag="M2A35_000352"
     CDS             complement(10878..11810)
                     /gene="glsA"
                     /locus_tag="M2A35_000352"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCB7196700.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            12072..14576
                     /gene="copA"
                     /locus_tag="M2A35_000353"
     CDS             12072..14576
                     /gene="copA"
                     /locus_tag="M2A35_000353"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCB7196701.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(14791..15132)
                     /locus_tag="M2A35_000354"
     CDS             complement(14791..15132)
                     /locus_tag="M2A35_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCB7196702.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            15270..16064
                     /locus_tag="M2A35_000355"
     CDS             15270..16064
                     /locus_tag="M2A35_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCB7196703.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            16268..16747
                     /gene="ybaK"
                     /locus_tag="M2A35_000356"
     CDS             16268..16747
                     /gene="ybaK"
                     /locus_tag="M2A35_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCB7196704.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(16784..18436)
                     /gene="ushA"
                     /locus_tag="M2A35_000357"
     CDS             complement(16784..18436)
                     /gene="ushA"
                     /locus_tag="M2A35_000357"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCB7196705.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     YFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            18654..19874
                     /gene="fsr"
                     /locus_tag="M2A35_000358"
     CDS             18654..19874
                     /gene="fsr"
                     /locus_tag="M2A35_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCB7196706.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            20112..21788
                     /locus_tag="M2A35_000359"
     CDS             20112..21788
                     /locus_tag="M2A35_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCB7196707.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGLVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(21921..23225)
                     /gene="gsk"
                     /locus_tag="M2A35_000360"
     CDS             complement(21921..23225)
                     /gene="gsk"
                     /locus_tag="M2A35_000360"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCB7196708.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLAGDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            23377..24336
                     /gene="aes"
                     /locus_tag="M2A35_000361"
     CDS             23377..24336
                     /gene="aes"
                     /locus_tag="M2A35_000361"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCB7196709.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(24333..25295)
                     /gene="hemH"
                     /locus_tag="M2A35_000362"
     CDS             complement(24333..25295)
                     /gene="hemH"
                     /locus_tag="M2A35_000362"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCB7196710.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(25531..26175)
                     /gene="adk"
                     /locus_tag="M2A35_000363"
     CDS             complement(25531..26175)
                     /gene="adk"
                     /locus_tag="M2A35_000363"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCB7196711.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(26356..28230)
                     /gene="htpG"
                     /locus_tag="M2A35_000364"
     CDS             complement(26356..28230)
                     /gene="htpG"
                     /locus_tag="M2A35_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCB7196712.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(28340..28945)
                     /gene="recR"
                     /locus_tag="M2A35_000365"
     CDS             complement(28340..28945)
                     /gene="recR"
                     /locus_tag="M2A35_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCB7196713.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(28945..29274)
                     /locus_tag="M2A35_000366"
     CDS             complement(28945..29274)
                     /locus_tag="M2A35_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCB7196714.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(29327..31258)
                     /gene="dnaX"
                     /locus_tag="M2A35_000367"
     CDS             complement(29327..31258)
                     /gene="dnaX"
                     /locus_tag="M2A35_000367"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCB7196715.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(29930..29994)
                     /gene="dnaX"
                     /locus_tag="M2A35_000367"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(31387..31938)
                     /gene="apt"
                     /locus_tag="M2A35_000368"
     CDS             complement(31387..31938)
                     /gene="apt"
                     /locus_tag="M2A35_000368"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCB7196716.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(32091..32468)
                     /locus_tag="M2A35_000369"
     CDS             complement(32091..32468)
                     /locus_tag="M2A35_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCB7196717.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSS
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            32538..33065
                     /gene="priC"
                     /locus_tag="M2A35_000370"
     CDS             32538..33065
                     /gene="priC"
                     /locus_tag="M2A35_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCB7196718.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            33079..33240
                     /gene="rsmS"
                     /locus_tag="M2A35_000371"
     CDS             33079..33240
                     /gene="rsmS"
                     /locus_tag="M2A35_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCB7196719.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(33452..36814)
                     /gene="mscK"
                     /locus_tag="M2A35_000372"
     CDS             complement(33452..36814)
                     /gene="mscK"
                     /locus_tag="M2A35_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCB7196720.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(36942..37589)
                     /gene="acrR"
                     /locus_tag="M2A35_000373"
     CDS             complement(36942..37589)
                     /gene="acrR"
                     /locus_tag="M2A35_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCB7196721.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            37731..38924
                     /gene="acrA"
                     /locus_tag="M2A35_000374"
     CDS             37731..38924
                     /gene="acrA"
                     /locus_tag="M2A35_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCB7196722.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            38947..42096
                     /locus_tag="M2A35_000375"
     CDS             38947..42096
                     /locus_tag="M2A35_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCB7196723.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            42642..43016
                     /gene="tomB"
                     /locus_tag="M2A35_000376"
     CDS             42642..43016
                     /gene="tomB"
                     /locus_tag="M2A35_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCB7196724.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            43042..43260
                     /gene="hha"
                     /locus_tag="M2A35_000377"
     CDS             43042..43260
                     /gene="hha"
                     /locus_tag="M2A35_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCB7196725.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            43432..43983
                     /gene="maa"
                     /locus_tag="M2A35_000378"
     CDS             43432..43983
                     /gene="maa"
                     /locus_tag="M2A35_000378"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCB7196726.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIAPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            44099..44569
                     /locus_tag="M2A35_000379"
     CDS             44099..44569
                     /locus_tag="M2A35_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCB7196727.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            44733..46283
                     /gene="pdeB"
                     /locus_tag="M2A35_000380"
     CDS             44733..46283
                     /gene="pdeB"
                     /locus_tag="M2A35_000380"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCB7196728.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(46325..46678)
                     /locus_tag="M2A35_000381"
     CDS             complement(46325..46678)
                     /locus_tag="M2A35_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HCB7196729.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(46798..46894)
                     /gene="ffs"
                     /locus_tag="M2A35_000382"
     ncRNA           complement(46798..46894)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="M2A35_000382"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            47057..47368
                     /locus_tag="M2A35_000383"
     CDS             47057..47368
                     /locus_tag="M2A35_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCB7196730.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(47399..47971)
                     /locus_tag="M2A35_000384"
     CDS             complement(47399..47971)
                     /locus_tag="M2A35_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7196731.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            48189..49049
                     /gene="tesB"
                     /locus_tag="M2A35_000385"
     CDS             48189..49049
                     /gene="tesB"
                     /locus_tag="M2A35_000385"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCB7196732.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(49097..50383)
                     /gene="amtB"
                     /locus_tag="M2A35_000386"
     CDS             complement(49097..50383)
                     /gene="amtB"
                     /locus_tag="M2A35_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCB7196733.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(50413..50751)
                     /gene="glnK"
                     /locus_tag="M2A35_000387"
     CDS             complement(50413..50751)
                     /gene="glnK"
                     /locus_tag="M2A35_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCB7196734.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(50932..52713)
                     /locus_tag="M2A35_000388"
     CDS             complement(50932..52713)
                     /locus_tag="M2A35_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCB7196735.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(52706..54478)
                     /locus_tag="M2A35_000389"
     CDS             complement(52706..54478)
                     /locus_tag="M2A35_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCB7196736.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(54508..54966)
                     /gene="decR"
                     /locus_tag="M2A35_000390"
     CDS             complement(54508..54966)
                     /gene="decR"
                     /locus_tag="M2A35_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCB7196737.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(55120..55938)
                     /gene="cof"
                     /locus_tag="M2A35_000391"
     CDS             complement(55120..55938)
                     /gene="cof"
                     /locus_tag="M2A35_000391"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCB7196738.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            56038..57738
                     /locus_tag="M2A35_000392"
     CDS             56038..57738
                     /locus_tag="M2A35_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCB7196739.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            57803..58498
                     /gene="queC"
                     /locus_tag="M2A35_000393"
     CDS             57803..58498
                     /gene="queC"
                     /locus_tag="M2A35_000393"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCB7196740.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(58550..58948)
                     /gene="fadM"
                     /locus_tag="M2A35_000394"
     CDS             complement(58550..58948)
                     /gene="fadM"
                     /locus_tag="M2A35_000394"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCB7196741.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(59042..59414)
                     /locus_tag="M2A35_000395"
                     /pseudo
     CDS             complement(59042..59414)
                     /locus_tag="M2A35_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            complement(59565..61436)
                     /gene="ppiD"
                     /locus_tag="M2A35_000396"
     CDS             complement(59565..61436)
                     /gene="ppiD"
                     /locus_tag="M2A35_000396"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCB7196742.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNEGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(61628..61900)
                     /gene="hupB"
                     /locus_tag="M2A35_000397"
     CDS             complement(61628..61900)
                     /gene="hupB"
                     /locus_tag="M2A35_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCB7196743.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(62109..64463)
                     /gene="lon"
                     /locus_tag="M2A35_000398"
     CDS             complement(62109..64463)
                     /gene="lon"
                     /locus_tag="M2A35_000398"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCB7196744.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(64651..65925)
                     /gene="clpX"
                     /locus_tag="M2A35_000399"
     CDS             complement(64651..65925)
                     /gene="clpX"
                     /locus_tag="M2A35_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCB7196745.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(66051..66674)
                     /gene="clpP"
                     /locus_tag="M2A35_000400"
     CDS             complement(66051..66674)
                     /gene="clpP"
                     /locus_tag="M2A35_000400"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCB7196746.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(66920..68218)
                     /gene="tig"
                     /locus_tag="M2A35_000401"
     CDS             complement(66920..68218)
                     /gene="tig"
                     /locus_tag="M2A35_000401"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCB7196747.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(68562..68879)
                     /gene="bolA"
                     /locus_tag="M2A35_000402"
     CDS             complement(68562..68879)
                     /gene="bolA"
                     /locus_tag="M2A35_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCB7196748.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            69184..69762
                     /locus_tag="M2A35_000403"
     CDS             69184..69762
                     /locus_tag="M2A35_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7196749.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            69806..71281
                     /gene="ampG"
                     /locus_tag="M2A35_000404"
     CDS             69806..71281
                     /gene="ampG"
                     /locus_tag="M2A35_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCB7196750.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLISG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            71742..72689
                     /gene="cyoA"
                     /locus_tag="M2A35_000405"
     CDS             71742..72689
                     /gene="cyoA"
                     /locus_tag="M2A35_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCB7196751.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            72711..74702
                     /gene="cyoB"
                     /locus_tag="M2A35_000406"
     CDS             72711..74702
                     /gene="cyoB"
                     /locus_tag="M2A35_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCB7196752.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            74692..75306
                     /locus_tag="M2A35_000407"
     CDS             74692..75306
                     /locus_tag="M2A35_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCB7196753.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            75306..75635
                     /locus_tag="M2A35_000408"
     CDS             75306..75635
                     /locus_tag="M2A35_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCB7196754.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            75647..76537
                     /gene="cyoE"
                     /locus_tag="M2A35_000409"
     CDS             75647..76537
                     /gene="cyoE"
                     /locus_tag="M2A35_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCB7196755.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            76722..77033
                     /locus_tag="M2A35_000410"
     CDS             76722..77033
                     /locus_tag="M2A35_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCB7196756.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            77017..77559
                     /locus_tag="M2A35_000411"
     CDS             77017..77559
                     /locus_tag="M2A35_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7196757.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(77615..78550)
                     /locus_tag="M2A35_000412"
     CDS             complement(77615..78550)
                     /locus_tag="M2A35_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCB7196758.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            78958..80322
                     /locus_tag="M2A35_000413"
     CDS             78958..80322
                     /locus_tag="M2A35_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7196759.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(80450..80941)
                     /gene="yajQ"
                     /locus_tag="M2A35_000414"
     CDS             complement(80450..80941)
                     /gene="yajQ"
                     /locus_tag="M2A35_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCB7196760.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            81109..82020
                     /gene="panE"
                     /locus_tag="M2A35_000415"
     CDS             81109..82020
                     /gene="panE"
                     /locus_tag="M2A35_000415"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCB7196761.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            81983..82573
                     /gene="yajL"
                     /locus_tag="M2A35_000416"
     CDS             81983..82573
                     /gene="yajL"
                     /locus_tag="M2A35_000416"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCB7196762.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(82627..84075)
                     /gene="thiI"
                     /locus_tag="M2A35_000417"
     CDS             complement(82627..84075)
                     /gene="thiI"
                     /locus_tag="M2A35_000417"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCB7196763.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            84281..84523
                     /gene="xseB"
                     /locus_tag="M2A35_000418"
     CDS             84281..84523
                     /gene="xseB"
                     /locus_tag="M2A35_000418"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCB7196764.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            84523..85422
                     /gene="ispA"
                     /locus_tag="M2A35_000419"
     CDS             84523..85422
                     /gene="ispA"
                     /locus_tag="M2A35_000419"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCB7196765.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            85447..87309
                     /gene="dxs"
                     /locus_tag="M2A35_000420"
     CDS             85447..87309
                     /gene="dxs"
                     /locus_tag="M2A35_000420"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCB7196766.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            87489..88463
                     /gene="yajO"
                     /locus_tag="M2A35_000421"
     CDS             87489..88463
                     /gene="yajO"
                     /locus_tag="M2A35_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCB7196767.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(88517..89035)
                     /gene="pgpA"
                     /locus_tag="M2A35_000422"
     CDS             complement(88517..89035)
                     /gene="pgpA"
                     /locus_tag="M2A35_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCB7196768.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(89013..89990)
                     /gene="thiL"
                     /locus_tag="M2A35_000423"
     CDS             complement(89013..89990)
                     /gene="thiL"
                     /locus_tag="M2A35_000423"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCB7196769.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(90068..90487)
                     /gene="nusB"
                     /locus_tag="M2A35_000424"
     CDS             complement(90068..90487)
                     /gene="nusB"
                     /locus_tag="M2A35_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCB7196770.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(90507..90977)
                     /gene="ribE"
                     /locus_tag="M2A35_000425"
     CDS             complement(90507..90977)
                     /gene="ribE"
                     /locus_tag="M2A35_000425"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCB7196771.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(91066..92169)
                     /gene="ribD"
                     /locus_tag="M2A35_000426"
     CDS             complement(91066..92169)
                     /gene="ribD"
                     /locus_tag="M2A35_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCB7196772.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSTQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQVL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(92173..92622)
                     /gene="nrdR"
                     /locus_tag="M2A35_000427"
     CDS             complement(92173..92622)
                     /gene="nrdR"
                     /locus_tag="M2A35_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCB7196773.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            92773..93312
                     /locus_tag="M2A35_000428"
     CDS             92773..93312
                     /locus_tag="M2A35_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCB7196774.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSALNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLKSQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            93611..94495
                     /gene="tsx"
                     /locus_tag="M2A35_000429"
     CDS             93611..94495
                     /gene="tsx"
                     /locus_tag="M2A35_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCB7196775.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(94672..95019)
                     /locus_tag="M2A35_000430"
     CDS             complement(94672..95019)
                     /locus_tag="M2A35_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCB7196776.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(95148..96119)
                     /gene="secF"
                     /locus_tag="M2A35_000431"
     CDS             complement(95148..96119)
                     /gene="secF"
                     /locus_tag="M2A35_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCB7196777.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(96130..97977)
                     /gene="secD"
                     /locus_tag="M2A35_000432"
     CDS             complement(96130..97977)
                     /gene="secD"
                     /locus_tag="M2A35_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCB7196778.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(98005..98337)
                     /gene="yajC"
                     /locus_tag="M2A35_000433"
     CDS             complement(98005..98337)
                     /gene="yajC"
                     /locus_tag="M2A35_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCB7196779.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(98360..99487)
                     /gene="tgt"
                     /locus_tag="M2A35_000434"
     CDS             complement(98360..99487)
                     /gene="tgt"
                     /locus_tag="M2A35_000434"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCB7196780.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(99543..100613)
                     /gene="queA"
                     /locus_tag="M2A35_000435"
     CDS             complement(99543..100613)
                     /gene="queA"
                     /locus_tag="M2A35_000435"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCB7196781.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            100706..101287
                     /gene="acpH"
                     /locus_tag="M2A35_000436"
     CDS             100706..101287
                     /gene="acpH"
                     /locus_tag="M2A35_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCB7196782.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(101292..103109)
                     /gene="malZ"
                     /locus_tag="M2A35_000437"
     CDS             complement(101292..103109)
                     /gene="malZ"
                     /locus_tag="M2A35_000437"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCB7196783.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQKIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(103265..104638)
                     /gene="proY"
                     /locus_tag="M2A35_000438"
     CDS             complement(103265..104638)
                     /gene="proY"
                     /locus_tag="M2A35_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCB7196784.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(104714..106033)
                     /gene="brnQ"
                     /locus_tag="M2A35_000439"
     CDS             complement(104714..106033)
                     /gene="brnQ"
                     /locus_tag="M2A35_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCB7196785.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(106440..107735)
                     /gene="phoR"
                     /locus_tag="M2A35_000440"
     CDS             complement(106440..107735)
                     /gene="phoR"
                     /locus_tag="M2A35_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCB7196786.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(107793..108482)
                     /gene="phoB"
                     /locus_tag="M2A35_000441"
     CDS             complement(107793..108482)
                     /gene="phoB"
                     /locus_tag="M2A35_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCB7196787.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            108672..109874
                     /gene="sbcD"
                     /locus_tag="M2A35_000442"
     CDS             108672..109874
                     /gene="sbcD"
                     /locus_tag="M2A35_000442"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCB7196788.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
BASE COUNT        26226 a        29343 c        27731 g        26610 t
ORIGIN      
        1 aaggtccgtc tcgccgggct gtacttttgc accgagcagc gggttcaccg ggttgcccga
       61 ggtcatcccg cccggacaca ccgagcacgc cacgccagtt ggcgcgccaa ttcttacacc
      121 tgccgaaccc tggacaatgg gaccgccata ctgagtcata tctccctgac gcgccgctgg
      181 ttttccgctc atataaagtg cccttttagt tattgttcat atccctgtgt tagcaacaaa
      241 aaaaataaaa tgacacacaa gaccagctaa tgtatttata tatttttcac ataaacttat
      301 ttgttctcaa tcaacaacaa ctgttattac aggtcacttt caatatttta ttaaactaaa
      361 gtttttagca cagatgaatt catcatcaac aaattcacta tatggataat atccattcaa
      421 cgcatatgat ttatgatttt attagtctga gttatttttg tagggctata tttaaaatta
      481 ttaaacccca ccgattcaga ttaatatcgc aatagaaaga taggaaatca ccaattcaat
      541 aacatcatca atgaaacata ttgtagaatt aatttaaact aggcgttcca ttcaaccaga
      601 acgcccctta tttactctct tcaccccgca aactgcctga acagcgcctt acccttaacc
      661 agtcgcgcac ccagccagcc gccgaaaagc gacagcagca gcgcaccgct gcacggcaga
      721 acaatccaca atcgccagtc tggctcccac ggaaaatcaa acactttcgc ctgcaacact
      781 gccagcgccg tttccgcacc aattgcggct accaggccag aaacaaaccc aagcatggcg
      841 aactcacacc acaatgtcgt gcgcagcagc tttttccccg caccgagtgt gcgccacacc
      901 accagctcca gatgacgctg acgcattccc acctgcacct gagccagcaa cagcaacata
      961 ccgcaggcgg tgaccagtac caccatcact tccagcgccc gacttacctg ctccagcacc
     1021 tgaccgacct gttttaaaat cgcgccaata tctaacagac taatggtcgg gaactggcgg
     1081 ttgagttgtg tcaacatgcc gttgccattc tcccagcgga aactggtaag ccagctctgc
     1141 ggttgcccgt ctaatgcccc ttcagggaaa ataaaataga aattaggccg cagactttcc
     1201 cagtccactt tgcgcaggct ggtcacttta gcgcggaact cctgggtatc gcccataaaa
     1261 gtcacggtat caccgagggc aacgtttaag cgtttcgcca gcccctcttc catcgacact
     1321 tcatcggctt ttggcggcca gttaccggcg acaatcggat tatgatcggg ccgcgtattt
     1381 tgccaggtaa gattgagttc gcggttaagc gcctcatctt cattaccttc tgtcggctta
     1441 tcgttaatcg ccgtcagccg cgcccgcacc accggataaa acgattccgg gactatctga
     1501 tgttccgcga ggaacgcttt tagcggtgct acctgttctg tggcgatgtt aattaaaaag
     1561 tagttcgggc tttctggagg tagctgctgc tgccagcggt cgagcagatc accacgcaac
     1621 accagcagca gtgccaacag cataaaggag agcgaaaatg ccgaaagctg gcttaacgtt
     1681 gaccacggct gacgtaacag gcggctaacc gccaggcgca gaggcagcga tttcagcgtc
     1741 atgcggcgaa gtacattcag cagcatccag cctagaacac cgcacagcaa agccagtact
     1801 accgcgcccg ccagcaccgc ccagagcagc atactgccgc ccattaatcc ggcgagcagc
     1861 agcacaacca ccacactgac aatcggcaga taaaacttca gcggccagac gttcgctacc
     1921 acatcattac gtaatacgcg taaaggctgc gttgccagca acaagcggta cggtcgtagc
     1981 cccaccagca gcgagatgac tgtcatggtg ccgagcgccc acagccacgg ccagaggctg
     2041 gctggcggta gtgcagcagg cagaacgggc ttgagcagca ccatcaacac gttttcgaac
     2101 aacaggccta tcgccccacc ggtaacggcc gaaagcaaca gcaccatcaa ccactgaccg
     2161 acgattagct tacgcagttg cgctcgccct gcccccagcg ttttgaggat cgccaccaga
     2221 tcgtagcgac tgcgacagta atgattcatc gctaccgcca ctgctgccac tgccagcagc
     2281 aaggtcagaa gcgccgaaag cagcaggaac tgttgcgagc gttccatcga tcgccccagc
     2341 gcgccttcgt cctgttccag accgtaccag cgttgttcgg gtttaagctg aggtaacaac
     2401 catttctcat agccgtcgag ctggttctcg ctgccgccga atttatagcg ccaggtgacc
     2461 cgactccccg gctgcacggc tccggtttta tcgacatccg ccagattcat catcagacgc
     2521 ggagccatct ggaaggggtt aaaaccggaa tccggttcct gaatcacttc tccggcaatc
     2581 cgcaaggtgg catcgcccac gtcaatggtg tcgcccgttt tcaggttaag cagcgccatc
     2641 aggcgtgggg ccagcaatac gctgcccgcc tgcggtttca ggccaggggg attagtttgc
     2701 agatcgccat acatcgggta gatatcatcc accgctttga cgttcgccag ctgcggtgtg
     2761 tcgcctgcaa atgtcatggt ggcgaaagtc agctgcttgc cgactttcag gccgcgcttt
     2821 tgcgcttcct ccagccatgc ttgcggcact tcgcgcgaac tgcgcaacgc ccgatcgccc
     2881 gccataaact cccggctttg ttggcttaag cccttctcca tgcgatcgct gatattgccc
     2941 agcgccagca cgcaggccac cgccaggctt agcgccagcc agacaattaa tagcgacggc
     3001 gaacgccatt cgcgccagaa ccaacgtgca atcatgcttc ctcctgcaac tgcccgttca
     3061 ccagccgtaa gcagcggtca cagcgtgccg ccagttgcag gtcgtgggtc accataatca
     3121 acgtggtgcc atgttcacgg ttgagggaaa acagcaggtc ggcaatttta tcgcccgtct
     3181 ggcggtcaag gttgccggtg ggttcgtcgg caaacagcac atcaggtcga ccattaaagg
     3241 ctcgtgccag cgccactcgt tgctgttcac cgccggaaag ctgtgccgga agatgaccca
     3301 gacgtttacc cagccctaac tgttcgagca acgctttcgc cccgttacga ctttccgcgc
     3361 tactctcacc gcgcagcaga gccggaagct cgacgttttc cagcgcgtta agggtaggaa
     3421 ttaacataaa tgactgaaaa acaaagccga cgtgcttcgc gcgcaacttt gcccgcgctt
     3481 cttcgtccat attatgtagc ggttgtccca ccagactcac ttcgccactg ctgccgtcat
     3541 caagcccggc gaggatcgcc agcaaggttg acttacccga tcccgactcg cccaccagtg
     3601 cgatggtctc gccacgtttg acaaccagct caactccggt gaggatggag agttcatgct
     3661 ccccctgacc gacggacttc ttaagatgat gaacttcaac aatgttttcc gctggcattt
     3721 gcccttcctg ttcctggtcc tgttaacctt ccgtgccgcc gcagcggaca cgttattgat
     3781 tctgggtgat agcctgagcg ccgggtatcg aatgtctgcc agcgcggcct ggcctgcctt
     3841 gttgaatgat aagtggcaga gtaaaacgtc ggtagttaat gccagcatca gcggcgacac
     3901 ctcgcaacaa ggactggcgc gccttccggc tctgctgaaa cagcatcagc cgcgttgggt
     3961 gctggttgaa ctgggcggca atgacggttt gcgtggtttt cagccacagc aaaccgagca
     4021 aacgctgcgc cagattttgc aggatgtcaa agccgccaac gctgaaccat tgttaatgca
     4081 aatacgtctg cctgcaaact atggtcgccg ttataatgaa gcctttagcg ccatttaccc
     4141 caaactcgcc aaagagtttg atgttccgct gctgcccttt tttatggaag aggtctacct
     4201 caagccacaa tggatgcagg atgacggtat tcatcccaac cgcgacgccc agccgtttat
     4261 tgccgactgg atggcgaagc agttgcagcc tttagtaaat catgactcat aaagcaacgg
     4321 agatcctgac aggtaaagtt atgcaaaaat cggtcttaat taccggatgt tccagtggaa
     4381 ttggcctgga aagcgcgctc gaattaaaac gccagggttt tcatgtgctg gcaggttgcc
     4441 gaaaaccgga tgatgttgag cgtatgaaca acatgggatt taccggcgtg ttgatcgatc
     4501 tggattcacc agaaagtgtt gatcgcgcag cagacgaggt aatcgccctg accgataatt
     4561 gtctgtatgg gatctttaac aatgccggat tcggcatgta tggccccctt tccaccatca
     4621 gccgtgcgca gatggaacag cagttttccg ccaacttttt cggcgcacac cagctcacca
     4681 tgcgcctgtt acccgcgatg ttaccgcacg gtgaagggcg tattgtgatg acatcatcgg
     4741 tgatgggatt aatctccacg ccgggtcgtg gcgcttacgc ggccagtaaa tatgcgctgg
     4801 aggcgtggtc agacgcactg cgcatggagc tgcgccacag cggaattaaa gtcagcctga
     4861 tcgaacccgg tcccattcgt actcgcttca ccgacaacgt caaccagacg caaagtgata
     4921 aaccagtcga aaatcccggc atcgccgccc gctttacgtt gggaccggaa gcggtggtgg
     4981 acaaagtacg ccatgctttt attagcgaga agccgaagat gcgctatccg gtaacgctgg
     5041 tgacctgggc agtaatggtg ctcaagcgcc tgctgccggg gcgcgtgatg gacaaaatat
     5101 tgcaggggtg agttgaagcg cgcgcttaag cccccatgtc aaagaaaata tcgacaacag
     5161 agagcgactc catgtccgta gaaaatattg tcaacattaa cgaatctaac ctgcaacagg
     5221 ttcttgaaca gtcgatgacc actccggtgc tgttctattt ttggtctgaa cgtagccagc
     5281 actgtttgca gttaaccccg atcctggaaa gcctcgcggc gcagtacaac gggcaattta
     5341 ttctggcgaa gctggactgt gacgcggagc agatgattgc cgcccagttt ggtctgcgcg
     5401 cgattccgac cgtgtatctg ttccagaacg ggcaaccggt agatggcttc caggggccgc
     5461 aaccggaaga ggcgatccgc gccctgctgg ataaagtgct gccgcgcgaa gaagagctga
     5521 aagcgcagca ggcgatgcaa ctgatgcagg aaggcaatta caccgatgcc ctgccattgc
     5581 tgaaagacgc ctggcagttg tcgaatcaga acggggagat cggcctgctg ctggcagaaa
     5641 cgctgattgc gctgaaccgt tctgaagatg cggaagctgt gctgaaaacc attccgttgc
     5701 aggatcagga cacccgctac caggggttgg tggcgcaaat cgaactgctg aagcaggcgg
     5761 ctgatacgcc ggaaattcaa cagttgcaac agcaggtggc ggagaatcca gaagatgccg
     5821 cactggcgac gcaactggct ctgcaactgc accaggttgg gcgcaatgaa gaggcgctgg
     5881 agttgctgtt cgggcatctg cgtaaagatc tcaccgccgc agacggtcag acacgtaaaa
     5941 cgttccagga gatcctcgct gcgctgggta cgggtgatgc actggcgtcg aagtatcgcc
     6001 gccagctgta tgcattgttg tattgatatt ccgttgatcg gcacatgaac cacaacgccg
     6061 gatgcgatgc tgcgcatccg gcatcgcatc atttcttctt caattgcgtc accaccaact
     6121 ggtggcgcga attataaaac ttacgatagg ttaaatagca ggcaataatg gtcgacaagc
     6181 tggcggttga gagcagcata aaggtcacca taatctgata tttaatcgcc ttcaccggat
     6241 caatcccggc aaatatcagc ccggacatca ttcctggtaa actcactaag ccaaccgttt
     6301 ttgctgaatc gaccgtcgga attaaagccg cgcgaatact gtcgcgaatc aatatcgctg
     6361 aagcctgctt cggcgtcgca ccaagactca gtttctcctg aatctgttgc tgttcgctaa
     6421 tgacccgttg ccctaaattg ttgtaacaca accccaccgc taccatggcg ttaccggcaa
     6481 tcatcccggc gatagggatc acctgcatcg ggataaattc aatcgaccca gagagaatca
     6541 gcaccgccag ggtaattccc gccccgaccg taatggcaat aaacgatgag ataaaagctt
     6601 tagcaatata tttactgcgt ttttgcgcgt tccacgccgc attaaagcag ataaataaca
     6661 ccatcaataa tgtcaggctg gcatcatcca cgctgaaaat atacttcagc acatagccga
     6721 caataatcag ctgaattatc gctcgcccga cgctccagaa aatatctttc tccagcgcca
     6781 gtttttcttt atggctaatt aagattgcca ccaccaccag cattaatgcc agtgccaatg
     6841 attcgttagt aatattatgc gagttcatag cgtgcttcct gcatttctcc ggcatgcggt
     6901 tgcagtgtaa tcactttatc cgcatgatta atttcgtctt tatcgtgtgt cacccacagc
     6961 acggcaatat tttgctcgcg cacataacga tggatcatct cattaacgtt atgtttatta
     7021 ctttcatcca gcgcactggt gatttcatcc agcaataaaa ccttcggcat aaattgcagg
     7081 ttacgaatca gtgagatgcg ttgtttttca ccaccagata gctcggcgat atttttcgtc
     7141 aaaatgctgt ccggcaaagc gaagcgttcg agaaaatcga gaaaaatggc cgggtcaggc
     7201 tgctggttac ggatctgcca gggaaagatc agattatcgt ataccgtatc gccaaacagc
     7261 gtcggcgtct gggcgcagta agagacttgc tggcggtaga tttctggctt tagtgtgctg
     7321 acatcctcac cttcaaacag taacgttccg ctggttgggc tgatcaatga agcaactatt
     7381 tttagcagcg tacttttgcc acaaccggaa ggaccggtaa ttaacttaaa ttcgccagca
     7441 cgcagcgaaa aattgatgtt attaagaatc ttcgcatcac ccgccagata tcctacgttt
     7501 tgtagctgaa gcaaaggact attttcctgc atcactgttc cctttttctg atttttacta
     7561 aaaacagttt atccttcgca ggaataaggg ggaactctct ttcagtaatc aggtataatt
     7621 tgtttaatca agcagcaatt ttgctgatta tggacggtac aacaggaggt ttttccgatg
     7681 cttatcttta tcccgattct catttttgtc gcgctggtca ttgtcggcgc gggcgtcaaa
     7741 atcgtgccgc agggctatca atggacggta gaacgttttg gtcgctatac caaaacgtta
     7801 cagccggggc tcagtctggt ggtgccgttt atggatcgca ttggtcgcaa gatcaatatg
     7861 atggagcaag tgctcgatat cccttcccag gaagttatct cgaaagataa cgccaacgtt
     7921 accatcgacg cagtctgttt tattcaggtg attgacgcgc cgcgcgcggc ttatgaagtc
     7981 agcaatctgg agctggcgat catcaacctg accatgacta acatccgtac cgtgttgggt
     8041 tcaatggaac ttgacgaaat gctctctcag cgcgacagca tcaactcacg cctgctgcgt
     8101 attgtcgatg aagccaccaa cccgtggggg attaaagtca cccgtattga aattcgcgac
     8161 gtgcgcccac cggcagagct tatctcttca atgaacgcgc agatgaaagc ggaacgtacc
     8221 aaacgcgctt acattcttga agcggaaggg atccgtcagg cggaaatcct caaagccgaa
     8281 ggtgaaaaac agtcgcaaat cctgaaagcg gaaggcgaac gtcagtcggc gtttttacag
     8341 gctgaagcgc gtgaacgttc cgccgaagca gaagcccgcg ccaccaaaat ggtgtctgaa
     8401 gctatcgcct ccggtgatat tcaggcggtg aactacttcg tagcgcagaa atacaccgaa
     8461 gcgttacagc agatcggttc ctccagtaac agcaaagtag tgatgatgcc attagaggcc
     8521 agcagcctga tggggtcgat tgccgggatt gccgagctgg tgaaagacag cgccaacaaa
     8581 cggactcagc catgatggag ttaatggtcg ttcatccaca tattttctgg ctcagtctcg
     8641 gcggtttgct gctggcagcc gagatgctgg gcggaaatgg ttatttgttg tggagcggcg
     8701 tggcagcggt gattaccggc ctggtggtct ggctggtgcc gctgggttgg gagtggcaag
     8761 gggtaatgtt tgccatcctg acgctgctcg ccgcctggct gtggtggaaa tggttgtcgc
     8821 ggcgggtgcg cgaacaaaag cacagcgata gtcatttaaa ccagcgcggg cagcagctga
     8881 ttggccgacg ttttgtgctg gaatctccgc tggtcaacgg gcgcggtcat atgcgcgtcg
     8941 gtgacagttc atggcctgtc agcgccagcg aggatctcgg tgcaggtacg catgttgaag
     9001 tcattgcgat agaagggata accctacata tccgagccgt ctcgtcttaa tcaccctgcc
     9061 cgatgatgac agcagccgga gagattttcg ataatcgggc agtcggcgct gtcatcgcca
     9121 gggcaggcat tcgccagtgc cagcagctgg tcgcgcatgg attgcagctc ctcaatgtgt
     9181 cgttcgatct ccgccacctt ctccagcgtg cgccgtttga cgtcggcgct gtgccgctgc
     9241 gggtcgttaa acagattcac cagctcgccg ctctcttcca ggttaaagcc cacctgccgt
     9301 gcctggcgca gtaaggtcag ttcgttgaga tgctgctgcg tgtaggtgcg ataaccgttt
     9361 tcgctgcgca tcggcggcgt caccagcccc ttctcttcat agaagcgaat ggctttgctg
     9421 gtcaggccgg taatttttgc tacatcgctg atgttcatcg ttcgcgcaac gcctcctgtg
     9481 ctttgttaaa gggtttcaac agatattcaa gcacgttttt ttgccagtgc gaatatcgac
     9541 cgccaccaca ataatacagg aagtactgcg cacagagaga tgttatacgg ttttattgcg
     9601 cttcatgacc attgccacaa taaggctgag taagagtgag ccaataatcc atatcaacgc
     9661 acggctaccc tgggaccaca gactgtagat aaagccaacg atcaccaaca acatcaccaa
     9721 cgtaccaacg ataagaatcg gcaacgaggc gtgaatatca tgacgcaggc ggatcgccac
     9781 cacaaacacc gccaggtagc aaatcaaaaa ggtggcgctg gcaacactgg cgagtgagcc
     9841 taaatttagc gccgccgtca tcagcataat caacaccacg acaatgatgt tgccccaggt
     9901 actctgccgc cacagggatt tattcattag cttcggcagt tcgcgttcgc tgcccatgtt
     9961 gtccatgatg ttaaacacgg cgaacaggtt cgcgttaatg gctgaagccg tcgccagtaa
    10021 agcgccgatg acgacgatca cataacccac atgtccgagc agcggagaag cagcctgcgc
    10081 aacggcggta tcggcatatt tttctaactc taatgccgat acatcgctaa gcaaaaccag
    10141 tgccagcgag atataaagca acgtggtaac gccaatcgcc accagaaacg cccgcggcat
    10201 aatgacctgc ggatctttca ctttatccgc cgcgttcgcc atcatgccaa agcccgcata
    10261 ggcaaggaag gttatcccga tacaggagaa gaacgcaccg gagctggggg gcgcagagac
    10321 ggaaatatgc gccggttgca gcgaccagac accggcaata atcagcaata acaggatcat
    10381 cattttaatg ccgacgagga tcacttccag ccgccctacc gcatggttgc ttaaggagtt
    10441 gaaaagcgtc atcaccgcaa tgatccccaa cgcgtagagc aaaataaggt gctcctcctg
    10501 gctgccttca tgcaaaaact gcacggcata agcgccaaaa gcacgggcga ccatggcgat
    10561 gctcaccgcc agcgtcaaca ggtacagtaa cgagagcgcc agcgaaaaga cgccgttgcc
    10621 taatccgcga cgaaagaagt cgataatgcc gccattgctg ggatagctcg cccccagacg
    10681 cgcataggca taaccggaaa acatcgccac aataccgcca aaagcaaagg cgacccaggt
    10741 cgaggcttcc attagcaacg cagcctgccc cagcagcgcg aagatccccg cccccaccat
    10801 tgccccaata ccgatggaaa cgacattcca tagaccgagg ggtttgttac cgttattacc
    10861 ttccgtgttc atcatgatca gcccttaaac acgttatagc cgagttgatt agcgaccgat
    10921 gccaccattt tttgaccgcg aacactgttg ccttcttcgt ccagcggtgg tgagaacgcg
    10981 gcaattccca tcacgccagg gacgaccgcc agaataccgc cacctacacc gcttttgccc
    11041 ggtaaaccaa cgcgatacgc ccagtcaccg gagcgaccat acagcccttc catcatcatt
    11101 tcggccagaa tgtacggcac gttgtcggcc tgaagaacgc gttcatgcgt cagcggattc
    11161 acaccccccg ccgccagcgt cgcgccaagt gttgccagtt caacagtatt gaggagcgtg
    11221 gagcactgac gggtgtacac gtcacaggct tccattgcat cacaatagag atatccggcg
    11281 gagtacagca gccaggcaat ggcccggtta tggaagttgg ttgtttgttc cgactggttg
    11341 acttcgtcag agagcgctac ctgctcacca gccagttgct gttggatatg taaaattcgc
    11401 tgccagcgtt gttcaacatt ttcagcgtta atcaggctgg tggtggcaat ggcgccagca
    11461 tttaccagtg gcgacagtgg tttgccgcca tgcaactcta aggcgataac tgagttaaag
    11521 ggcaatccgg tcgggtcagc gccaattttg tcctgtaccg cctgcgggcc gacatcttct
    11581 aacgcaaggg ctaacgtaca gactttcgag atggattcca gcgcaaagcg gtaatcacta
    11641 tcacccgcgc gatagatgtt gccatcgcag gtcacaatag ccactgctgc cagttgacta
    11701 ggtacattcg ccagaaaggg aatgtagtcg gcattttgtc cgccgttaag tgagtgaaat
    11761 tgggtgtaag cctgatccac tgcctgctgt aatttgtttg catctaacat cttttgttaa
    11821 ctccttttta tagatgcggg aggtaattcc tcaccccggt gccgattttc aggcatcttg
    11881 atttaactta gcacccgcaa cttaactaca ggaaaacaaa gagataaatg tctaatcctg
    11941 atgcaagttg agccaatttt ttaatcttta cggactttta ctcgcctggt ttattaattt
    12001 cttgaccttc cccttgctgg aaggtttaac ctttatcaca gccagtcaaa actgtcttaa
    12061 aggagtgttt tatgtcacaa actatcgacc tgaccctgga cggcctgtcc tgcggtcact
    12121 gcgttaaacg cgtgaaagaa agtctcgaac agcgcccgga tgtggaacaa gcggatgtgt
    12181 ctatcactga agcgcacgtt accgggactg ccagtgcaga acagctgatc gaaaccatca
    12241 aacaagcggg ttatgacgca tctgtaagcc acccaaaggc taaaccgctg gcggagtcat
    12301 caatcccgtc ggaagcactg acagcggttt ctgaggcgct tccggcagcg accgccgatg
    12361 acgatgacag ccagcagttg ctgctgagcg gcatgagctg cgccagctgt gtcacccgcg
    12421 tacaaaatgc gctgcaaagc gtaccgggcg tcactcaggc acgggtaaac ctggcggagc
    12481 gtactgcgct ggtgatgggc agtgcctccc cacaagattt agtgcaggcg gtggaaaaag
    12541 cgggctacgg cgcggaagcg attgaagatg acgcgaaacg ccgtgagcgc cagcaggaaa
    12601 ccgccgttgc tacgatgaag cgcttccgct ggcaggcaat tgtcgcactg gcggtgggta
    12661 tcccggtgat ggtctggggg atgattggcg ataacatgat ggtcaccgct gacaaccgca
    12721 gcctgtggtt ggttatcggc ctgataaccc tggcggtgat ggttttcgcc ggcggccatt
    12781 tttaccgcag tgcatggaaa agcctgctga acggtgcagc gacgatggat acgctggtgg
    12841 cgctgggtac tggcgtggcg tggctctatt cgatgagcgt caacctgtgg ccgcagtggt
    12901 tcccgatgga agcgcgacat ctttattacg aagccagcgc gatgattatc ggtctgatca
    12961 atctcggcca tatgctggaa gcacgcgcac gtcagcgttc ttctaaggcg ctggagaaat
    13021 tactcgattt aaccccaccg acggcgcgcc tggttactga cgaaggtgaa aaaagcgtgc
    13081 ctctggcgga agtgcagcca ggtatgttgc tgcgcctgac gaccggcgat cgcgtgccgg
    13141 tagatggcga gattacccag ggcgaagcat ggctggatga agcgatgctg acgggcgaac
    13201 caatcccgca gcaaaaaggc gaaggcgaaa gcgtccatgc cgggacagtg gtacaggacg
    13261 gtagcgtgct gtttcgtgcc agtgcggttg gcagccatac cacgctgtca cggatcattc
    13321 gcatggtgcg ccaggcccag agcagcaagc cagaaatcgg tcagctggcg gataaaatct
    13381 cagccgtatt tgtacctgtg gtggtggtga ttgcactggt cagtgcggca atctggtact
    13441 tctttggtcc ggcaccgcaa attgtttata ccctggtgat tgccaccacg gtactgatta
    13501 tcgcctgtcc gtgtgcgctg gggctggcga cgccgatgtc gattatttcc ggcgtcgggc
    13561 gggcggctga gtttggcgtg ctggtgcggg acgccgacgc gctgcaacgc gccagtacac
    13621 tcgataccgt cgtgttcgat aaaaccggga cgctgaccga agggaagccg caggttgtcg
    13681 cagtgaaaac gtttgctgat gttgatgaag cgcaggcatt gcgtctggcg gcggcactgg
    13741 agcaaggttc cagccatccg ctggcacggg cgatcctcga taaagcaggt gatatgcagc
    13801 tgccgcaggt caacggtttc cgcacattgc gcgggctggg cgtgagcggt gaagctgaag
    13861 gtcatgcgtt attgctgggc aatcaggcgc tgttaaatga gcaacaggtt ggtaccaaag
    13921 ctatcgaagc ggagattact gctcaggcat cgcaaggggc aacgcctgtg ctgctggcgg
    13981 ttgacgggaa agcggtagcc ctgctggcag tacgcgatcc attgcgtagt gatagcgtgg
    14041 cggcgctgca acgcctgcat aaagcgggat atcgtctggt gatgttgacc ggggataacc
    14101 caaccaccgc caatgcgatc gccaaagaag ccgggattga tgaagtgatc gccggggtgc
    14161 tgccggacgg taaagccgaa gcgattaaac gcctgcaaag tgaaggacgt caggtggcaa
    14221 tggtgggcga cggcattaac gacgcgccag cgctggccca ggcggatgtc ggcattgcga
    14281 tgggtggcgg cagtgatgtt gccattgaaa ccgcggcgat taccctgatg cgccatagcc
    14341 tgatgggcgt tgcggatgcg ctcgcgattt cccgcgcaac gctgcgcaac atgaagcaga
    14401 acctgctcgg tgcctttatc tacaacagca tcggtattcc ggtcgccgcc ggtattttgt
    14461 ggccgtttac tggaacactg cttaacccgg tagttgccgg agcggcaatg gcgctctcgt
    14521 cgattaccgt agtgagtaac gccaaccggt tgctgcggtt taaaccgaag gaataagtac
    14581 gtcattgcgg atgcgctgct ttagggcggc gcatttggca tcgacgcatg actgccggat
    14641 gcggcgtaaa cgccttatcc ggcctacggt tcaggcacag gtttgtaggc ctgataagac
    14701 gggtaagcgt cgcatcaggc aatggtgctc ggatgccgga tgcggcgtga acgccttatc
    14761 cggcctacga gatgaagcga tgttttggcc ttacgcgacc ttttttgcac gctcttcacg
    14821 gcgtgccaaa tattcagcca ctgtttcaat ccgtcccaat tcttcctggg tgcgcatgtt
    14881 gttttcaact tcccaaagat caactgccgc ctggaggttt agcctaaaat cgactgtggt
    14941 atcaaaaact ttcgccagac ggaatgccat ctcagtggtg agtttacgat tgttattgat
    15001 cagtgcactg acgctattac gatgaacatg cagcaactct gctaactcat tgattttcaa
    15061 atcgagcggt tccagatatt catagagaag aatatctccg ggtgtcgtcg gttttcttgt
    15121 tgcctgtttc atggttttta actgttgctt ccctgtgaat agcgatgcaa gaacatactg
    15181 aatcatacaa acacattaaa caagtcccca actccatttg cgccatccct caccgcgcgc
    15241 tagcatgata tttcacaaag ggaggtcgta tggatctgtt gtaccgggta aaaacgcttt
    15301 gggccgcgct gcgcggtaat cattacacct ggcctgccat cgatatcacc cttcccggca
    15361 atcgccattt tcatctgatt ggcagtattc atatgggtag ccacgatatg gctcccctgc
    15421 ccacccgttt gctcaaaaag ctcaaaaacg ccgatgcgct gatcgtcgag gcggatgttt
    15481 ccaccagcga tacgcctttt gctaatttgc ctacttgcga ggcgctggaa gagcgcatta
    15541 acgaggagca actccaaaat ctgcagcaca ttagccagga gatgggcatt tctccgtcgc
    15601 tcttttctac tcaaccgctg tggcaaatcg cgatggttct tcaggcgacg caggcacaaa
    15661 aactggggct gcgggcagaa tacggtatcg attaccagtt attgcaggcg gcgaagcaac
    15721 aacataaacc cgtgattgaa ctggaagggg ctgaaaacca gattgccatg ttgctccagc
    15781 tccctgacaa aggactggcg ctgctggacg atacgctgac ccactggcat accaacgcac
    15841 ggttgctgca acaaatgatg agctggtggc tgaatgcacc gccgcaaaat aatgatataa
    15901 cgctgcccaa tacgttcagt cagtcgctgt acgatgtgct gatgcatcag cgaaatctcg
    15961 cctggcggga taaattacgc gccatgccgc cggggcgata tgtggtcgcg gtcggtgcac
    16021 tacacctgta tggagaaggg aatttgccgc aaatgttgcg ctaaaaaaat ggccaatatc
    16081 gctattggcc cgtcaaagag gaatttcatt tttttattat tatgccgtca ctttaagcga
    16141 cggtgtaact cgattgtcgc tcaatctcac catcctgcca atactcttag gcaagatgac
    16201 tctttttttt ggaccgccat caggcgtatg cttgcttcgt ttttttgttt aaggcaggaa
    16261 aattaccatg acacccgcag ttaaattact cgaaaaaaac aagatttcgt ttcaaatcca
    16321 tacctacgag cacgatccgg ctgaaaccaa ttttggcgat gaagtcgtca aaaaattagg
    16381 tttgaatccg gatcaggtct acaaaacgct gctggtggca gtgaacggtg atatgaaaca
    16441 ccttgccgtg gccgttacgc cggtcgcagg tcaactggat cttaaaaaag tagcaaaagc
    16501 gctgggtgcc aagaaagttg agatggccga tccgatggtc gcgcagcgtt cgacgggata
    16561 cctggttggg gggattagcc cactggggca gaaaaaacgt ctgccaacga ttatcgacgc
    16621 ccccgcacaa gaatttgcca ctatttatgt ttccggcggc aagcgcggac tggatatcga
    16681 actggcggca ggcgatctgg caaagatcct cgatgccaaa tttgctgata tcgcccgccg
    16741 cgattaagca ttgtgccgga tgcaaacatc cggcactttc ggattactgc cagctcacct
    16801 cacctttcgg ttcataaaca ctcacatcca gcggcgagct tttctggata tacgctttca
    16861 gcacttcggc atcaataaag ccggtattca catagcccgg tttgttatca aggcgcggat
    16921 atccatcacc gccggtggca ttgaagttta atgtcgccat acggtaagtt ttcgccggat
    16981 cgaccggttc gcctttgatt ttaaggtcgt tcagtttgcc gtctttcgcc acaaagctaa
    17041 cgttggcaaa ttgcgggtag gcacctgaat ctggcttcat ctgcgcgacg gcggtcaggt
    17101 aatcaatcac ctctttaccg gtcatgtcgg catacaccac cacattgccg aatggctgca
    17161 ctttcagcac gtttttatag ctgatatcgc ctgcttcgat agaatcacga attccgcctc
    17221 cgctcatcac cgcaaagtcg gcaccagtgc gatccatttg ggctgccaga atcaaccgcc
    17281 ccatattggt ctgtacaaaa cgcactttgt cacgatcgcc ttccagacga ccattggttt
    17341 cgcctatttt cacttccagc tgcgctttgc ctttgttctg gaacggtgat aacagcgaga
    17401 tcatttgctg gttttcagcg atttcaggag tgtaaagcac gcgctcgctt ttcccgtctt
    17461 cccaggtcac tttcttcttc aggttcaccg gaatcagctg gtagttaacc attttcattt
    17521 cgccattacg aaactcaaaa tcagcccgtc ccacgtattt gccccactca tgcgcctgca
    17581 caatccagat gccgttttgt tgatctggct tgcatggcgt acccggcacg taatcgacct
    17641 gttttttgtt ttctgccgcc atgcagaccg gatcttgcga gtgaccaccg acgatcatcg
    17701 ccagcgatcc ggcaggcagc gcgcgtgcca tctccacatc gcccggtgcg ttagagccgt
    17761 gctcaccatt atcgtaatgc cccatatggg tcgccgcgat aataatgtct ggcttttctg
    17821 tctgttgcag ctcctgaatc accagcttcg cttcatcggc gggcttacga aattcgacat
    17881 cagtgaagta ttccgggtta ccaatttttg ctgtgtcatc ggttgtcagc ccaatgacgg
    17941 caattttcag atcctgacgc ttaaacagcg cccacggttt aaacaggcgc tcgccagtac
    18001 ttttctggta gatattcgcg gaaagcaacg ggaacttggc ccacttttcc tgctggcgta
    18061 atacggtgag cggattatca aattcatgat taccgatcgc catcgcgtca tagcccacca
    18121 gattcatacc gcgaaaatca ggttcggcat cctgtaagtc agactcgggc acgccagtgt
    18181 taatgtcgcc accggaaagt agcagcacgc taccgccttc agccgcaacc tctttgcgga
    18241 taccatccac cagcgttttt tgcgccgcca gaccatattc gccatattca ttgcgccaaa
    18301 aatacccatg atgatcattg gtatgcagaa ctgtaatttt gtaggtttta tcctgctcat
    18361 acgccagagc agtttcactc gccagtgtaa atgtggttaa cagcgctaac gccacgcccc
    18421 gctgcaataa tttcatactt ctctccctga cctgatttca acgtgttgaa aaatagtatg
    18481 acgcgacatc atagacaagt ctgcgtccgg tgaagataaa tctgcatcca gattagaaat
    18541 tctgtattcg gcgttgagac ttccttcagg cgacgcatac aggtatgtta gtcgggtaat
    18601 aatcacaaat aagatcattc tttacatcgc aaccctgaaa aagtgacttt cctatggcaa
    18661 tgagtgaaca accccagcct gtggcgggcg cggctgcgtc aacgaccaag gcccgaacat
    18721 cgtttggtat tttaggtgct atcagcctct cacatctgct gaacgacatg atccaatcgc
    18781 tgattctggc gatttatccg ctgcttcagt cagaattttc tctgacattt atgcagattg
    18841 gcatgataac cctcaccttc cagctcgcct cttcgctact gcaaccagtg gtcggctact
    18901 ggaccgataa atatccgatg ccgtggtcgt tgccaattgg catgtgcttt accttaagtg
    18961 gtctggtgct gcttgcgctg gcgggcagtt ttggcgcagt tctgctggcg gcggcgctgg
    19021 tcggtaccgg ttcatcggtc tttcatccgg aatcttctcg cgtagcccgt atggcttccg
    19081 gcgggcggca tggcctggcg caatctatct ttcaggtcgg cggcaacttt ggcagttccc
    19141 tgggaccctt gctggcggcg gtgattatcg cgccttatgg caaaggcaac gttgcctggt
    19201 ttgtgcttgc ggcactgctg gcgatcgtgg tgttggcgca aattagccgt tggtactcgg
    19261 cacagcaccg aatgaataaa ggaaaatcca aagcgacgat aatcaatcca ctgccgcgca
    19321 acaaagtggt actggcagtc agcattctgt taatcctcat tttctcgaaa tatttctata
    19381 tggcgagcat cagcagctat tacacctttt atctgatgca aaaattcgga ttatctatcc
    19441 agaatgccca gcttcatctg tttgccttcc tgtttgccgt tgcggcaggt acggtgatcg
    19501 gcgggcctgt aggggataaa attgggcgga aatatgtgat ttggggctct atcctcggcg
    19561 ttgcgccgtt tacgctgatt ttaccctacg ccagcctgca ctggacgggg gttttaacgg
    19621 tgattattgg atttatcctc gcttcggcat tctctgccat tctggtctac gctcaggagc
    19681 tacttccggg acgtatcggt atggtttctg gactcttttt cggttttgct tttggcatgg
    19741 gaggtctggg agcggcagtt ctggggctta tcgccgatca caccagcatc gagttagtct
    19801 ataaaatctg tgctttcctg ccactattgg ggatgttgac catattcctg cctgataacc
    19861 ggcataaaga ctgatttcat tgcagccaaa ggcaaacttt ggctgcatcg ttttacagtc
    19921 gccataagcc tttcctctgt taaaccgcct tctgttttcc gatccgtgct ttttttgcag
    19981 ctaattccat ttttctgcaa aattcaataa ttattcatga acatcatcaa tgaaattgtc
    20041 ataaactatt acctgtaatt ttggttttcc cggccaaaaa tggacaataa ccaccaggaa
    20101 aaggagacgg aatgcatcac gccaccccgc ttatcaccac cattgttggc ggccttgtgc
    20161 tcgcctttat cctcggcatg ctggccaata aactacgtat ttctcctctg gtgggatatc
    20221 tgttagcggg tgtgctggca ggaccattca ctccgggctt tgttgccgat accaagcttg
    20281 cgccggaact ggctgaactg ggcgtcattc tgttgatgtt tggcgtcggt ttgcactttt
    20341 cgctgaagga tttgatggcg gtaaaggcca tcgccattcc cggtgcgatc gcccagatag
    20401 ccgtggcgac gctgctgggt atggcgctct ctgccgtgct gggctggtcg ttaatgaccg
    20461 gtctcgtgtt cggtttatgt ctttccaccg ccagtaccgt ggtgttactg cgcgcacttg
    20521 aagaacggca attaattgac agtcagcgtg ggcaaatcgc catcggttgg ttgattgtgg
    20581 aagacctggt aatggttctg acgctggtgt tgctgcccgc agtggcagga atgatggaac
    20641 agggcgatgt gggctttgcc actcttgcag tcgatatggg gatcaccatc ggcaaagtga
    20701 tcgcatttat cgccattatg atgctggtag gtcgccgtct ggtgccgtgg attatggcac
    20761 gcagcgcggc aaccggttct cgcgagctgt ttaccctgtc ggtgctggcg ctggcgttag
    20821 ggattgcctt tggtgcggta gagctgtttg atgtctcctt tgcactcggt gcgttctttg
    20881 ccgggatggt actgaacgag tctgaactga gtcaccgtgc cgcccacgat acgctgccat
    20941 tgcgcgacgc gtttgcggtg ctgttttttg tctccgtcgg gatgttgttt gatccgttaa
    21001 ttctgattca gcaaccgctg gcagtgctgg cgacgctggc gattattctg tttggtaagt
    21061 cgttagccgc atttttcctg gtgcgactgt ttggtcactc ccaacgtacg gcattaacca
    21121 tcgccgccag cctggcgcag attggtgagt tcgcgtttat cctggcggga ctgggaatgg
    21181 cattgaattt actgccgcag gccggacaaa acctggtact ggcaggggcg atcctgtcga
    21241 ttatgctcaa cccggtactg ttcgcactac tggagaaata tctggcgaag accgaaacgc
    21301 tggaagagca gacgctggaa gaggcaatcg aagaagagaa acagatccca gtggatattt
    21361 gcaaccatgc gctattggtg ggttacggtc gtgtaggcag cctgctgggg gagaaattgc
    21421 tcgcctctga tattccgctg gtggtgattg agacgtcacg aacccgtgtt gatgagctgc
    21481 gagagcgtgg ggtccgcgct gtattgggca atgcagcgaa cgaagaaatt atgcaactgg
    21541 cgcatctgga atgtgcaaaa tggctgatcc tgacgatccc caacggttat gaagcgggtg
    21601 agattgtggc gtctgcccgc gcgaaaaacc cggatattga gattatcgcc cgcgcccatt
    21661 atgacgatga agtgacgtat atcaccgaac gtggcgcgaa tcaggtagtg atgggtgaac
    21721 gtgaaattgc ccgtactatg ctggaactgc tggaaacgcc accggcgggt gaagtggtga
    21781 cggggtaaac ggtggttgcc ggatgtaaaa tatgaaaaat atgtaggcat gataagacgc
    21841 gccagcgtcg catcgggcat cgagagccaa atgccagatg cggcgtgaac gccttatctg
    21901 gcctacgaac cgacgataac ttaacgatcc cagtaagact cttccaggct gtcttcacgc
    21961 tccggcaagc cgcgcgttaa acgaggtgaa tgctggttca gtacctgata gctcacacgg
    22021 ttagcatatt tacacacctg cgctaacgat gaataagtta accaggtgaa tttatgtttg
    22081 ctggagtttg gtacgttgct acgatggtag ctgttggcgg taatgtcatg cagcaacgct
    22141 gccaatgcgc catcccccgc tccattagtg ttcatgattt tttccggccc gcccatgtac
    22201 ggcgcaatgt gcgaatatac acgcagcgga ttctggcaat ccttgtggcg catggcgcgg
    22261 ctaaactcat actggttgaa ttccgctata gcgcccggca gcagcggatg ctgggtttta
    22321 cgtttcgctt cgtcttcggt aaagcccgcc atatacaagc cgattggccc ggcggtgcac
    22381 agcaccagat ctacccagtc cagcgccttg tcagatgcca acaacggatc gctttctccg
    22441 gtcaacgctt cggcttcatc ttcgttcatc gcaaggatag aaacgtggtc tttgaggaat
    22501 tgctgccacc actgcggatt ctcggcaatg acaaacttgg tgcccagcgt cagcaccacc
    22561 ggtacgttat atttcttcgc gtactcaatg gctttcatgg ttgcttccgg catgggttca
    22621 cccggtttgc aacgcaccag ataagaggtg agaaccagtg ccgaggctcc ggcaatcaca
    22681 tcttccggaa tgctttcagc ccgcagctgg ttcatgtggc ctggactgat agcaaaggta
    22741 cgttccccgg actcgccaat cagcgtaaag caacgaccaa tcgggccatc cacgccttgt
    22801 agatagttaa gatcggtacg gctggaagtg ttacacaggt aacgataggc ataactgcca
    22861 atttcaatat tgctgcacat gacgcccagc agcaccgaac ggtcgcccgc gagcaccgag
    22921 tagttgtgca tggtgttacc aatggtgcca cccgcaaact gatgggtaat caggtttttc
    22981 tgttttagct cctgataaag cgcttcggct acgtcatcct caatcaccag tgaatgcccg
    23041 gcgcttaatc cataacgctc aatgaattca tcatccactt tcgcttcaat atcgaccagc
    23101 gtctggtcaa tacctacgac ccaggcggcg ctggtttcgt tttctggctg gaattgctga
    23161 agcagcggat cgcgtgcatt tacagggaag taatgtttgg atttacgttt accgggaaat
    23221 ttcatggttg tttttacggg tggttaactg agcggcgaat gttaacacaa tctgacggga
    23281 gcgcgcgaga cgcagccagt ttcaatatca gcaggcggga tcgcattttc atggcagtgg
    23341 ttccttacaa tgacgtaatt tgaaaggagt ttttgtatga agccggaaaa caaactacct
    23401 gttctggacc ttatttctgc tgaaatgaag accgttgtga atactcttca gccggattta
    23461 ccgtcctggc ccgcaacggg aacgattgct gagcaacgac agtattacac gcttgagcgc
    23521 cgattctgga atgcgggcgc tccagaaatg gcaaccagag cttacatggt tccaacaaaa
    23581 tatgggcagg tggaaacacg tctcttttgt ccgcagccag atagcccggc gacgctattt
    23641 tatttgcatg gaggcggttt tattctcggc aatctcgata cccacgatcg catcatgcgc
    23701 ctgctggcaa gctacagcca atgtacggtg attggtattg attaccccct ttcacctgaa
    23761 gcgcgttttc cgcaagcgat agaggaaatt gtggctgctt gttgttattt ccaccagcag
    23821 gcggaggatt atcaaatcaa tatgtcccgc attggctttg ccggtgattc cgcaggtgcc
    23881 atgctggcgc tcgccagtgc gttgtggttg cgtgataaac agatcgattg cggtaaaatt
    23941 gcgggtgttt tgctgtggta tgggctttac ggattacggg attccgtgac tcgtcgtctg
    24001 ttgggcggtg tctgggatgg cttaacgcaa caggatttgc agatgtatga agaggcgtat
    24061 ttaagcaacg acgcggaccg cgagtcaccg tattactgtc tgtttaataa tgatctcact
    24121 cgcgaagttc cgccctgttt tattgccggg gcggagttcg atccgctgct ggatgacagc
    24181 cgtctgcttt accagacgtt agcggcgcat cagcagccct gtgagttcaa actctaccca
    24241 ggtacgctgc atgccttttt gcattattca cgaatgatga aaaccgccga cgaggctctt
    24301 cgcgacggcg ctcagttctt taccgctcag ctttagcgat acgcggcaac aagattagcc
    24361 atcatttcga tatgttccgg cgtggcatta agcgctggaa tatattcata ttttttcccg
    24421 ccggcaccga ggaagacctc acggttttgc tcggcaatct cttccagcgt ctccagacaa
    24481 tccgcagcaa agcccgggca catcacctgt atatgaccta cgcctttttc tccgagcatt
    24541 ttcagcgttt cgtcggtata aggcatcagc cagggttccc ggccaaagcg cgactgaaag
    24601 gtcatcatca ctttttccgg tgccatcccc agtgcggaag ccagttcgcg agtcgttgtg
    24661 cggcaacgtt gcgggtaatc atcgccttca tctgcataac gctggggaat gccatgataa
    24721 gagagcagca gcagatccgg ttcgccatgt ttggcaaaag aagcgcgtac gctgttcgcc
    24781 agtgcattaa tgtaatcgtg gttatcggcg taatcacgaa taaacgatat ccccggaatg
    24841 ctacgtttgc gcgccagaat gcgtgccagt tcatcccata ccgcaccgac cgtagaacag
    24901 gagaattgcg gataaagcgg cagcaccaca atatgatcta catgctctgc caggagttca
    24961 tcgacggcgc tttccagtga tggcgagccg tagctcatgc ccagcgctac gggcgtctcc
    25021 ggtaaacgtt gtgccagcgc ctgctgttgc tggcggctgt aaaccatcag cggcgagccg
    25081 tcttccatcc agaccgaggc gtacagcttc gccacacgcg gcgagcgcag cggcaaaatc
    25141 acgccgcgca gcaacggcca ccataacaac cgtgaggtat caaccacgcg tctgtcgctt
    25201 aaaaattgtt tcagatagcg ttttaccgct tcaggtgtgg gggcatcggg cgtacccagg
    25261 tttgccagca ggataccggt tttagtctga cgcattaccg cctcttatcg attcaacttg
    25321 ttgataattg tagcggaaaa gtgaggaaga agaactaatt gctgtgagag gtcaaatcag
    25381 aaaaaggaat agcgcagatt gaggtttttg taaaaggccg aatgaatatc cggcctgaga
    25441 ttgctgataa gtttgcttgt gcgggcctgt tcccctcacc ctaaccctct ccccgagggg
    25501 gcgaggggac tgtccgtgcg cgctttcgaa ttagccgagg attttttcca gatcagcgcg
    25561 aacttcagca accggcttgg tgccgtcaac tttcgcgtat ttggtgttac ccgcttccgc
    25621 ttctttggag tagtagccga tcagcggtgc agtcatctga tggtattcaa ccagacgttt
    25681 acgcacggtt tcttcctgat cgtctttacg ggtagtcagt tcttcaccgg taacgtcgtc
    25741 tttgccttct actttcggcg gattgaattt aacgtgataa acacgaccag acggcgcatg
    25801 aacgcggcgg cctacgatac gatcaacaat cagttcgtcc ggtacgtcga attccagaac
    25861 gtaatcaaca ttgatgcccg cttctttcat cgcgtctgcc tgcggaatgg tacgcgggaa
    25921 gccgtccaac aggaaaccat tacggcagtc ttcctgagca atgcgctctt taaccagcgc
    25981 gatcaccagt tcgtcggtga ccagtttgcc agcatccata atgtcttttg cttgtttacc
    26041 cagctcggag ccagatttga ccgcagcacg cagcatatcg ccagtggaga tttgcggaat
    26101 accatatttc tccatgatga actgagcctg agtccctttc cccgcgcccg gagcgccaag
    26161 cagaatgata cgcattgcga aaatcccctt aaaatgtgtc gaattttttg aaaaagcgat
    26221 aaacgatacc accaacaggc gattgcctca agaaaggcac ctgaggctga aacggttaat
    26281 ggcgagataa tttgcggaaa gtgcagatga tgaaaagaaa aatgccggat gacacaaagg
    26341 tcatccggca tgacatcagg aaaccagcag ctggttcata cgacgaataa acaggttcgg
    26401 atcttccagc gtgccgcgtt ctgccagcag cgcctgatcc agcagcagtt ctacccactc
    26461 gctgaacttc gcttcatctt cagtatctgc cgcacgtttc accagtacgt gatccgggtt
    26521 cagttcgaag atgtatttca cttctggcac tttctggccc gccgcagcga acagtttcgc
    26581 catctgagtg ctcatttcgt ccgcgtcggt cgaaacgatc gctggcgtat cggtcagacg
    26641 gtgagtcaga cggacatctt tcacgcgctc gccgagcagg gctttcacac ggtcgatgaa
    26701 cggagtcagt gctttctccg cttctttcgc gctctcatca acttcgtcag ccagtttttc
    26761 cagcgactcg tcaactttag acaccgactg gaacggttta ccgtcgaact cagtcagata
    26821 gttcatcatc cactcatcga tgcggtcgga aagcagcaga acttcgatgc ctttcttacg
    26881 cagcagttcc aggtgcgggc tgctcttcgc tgccgcatag ctgtctgcag tgatgtagta
    26941 gattttctcc tgcccttctt tcatgcggga aacgtagtct tccagagaca cggtctgcgc
    27001 agaagaatcg gtataagtag aagcaaaacg cagcagtttg gcgatcgctt cctggttagc
    27061 gaaatcttcc gccggacctt ctttcagtac caggccaaac tgttgccaga aggtctggta
    27121 tttgtccgcg tcgtctttcg ccagtttttc cagcatttgc agcacacgct tggtcagcgc
    27181 attgcgcagg ttacgcgtta ccgtgctgtc ctggaggatt tcacgggaaa cgttcagcgg
    27241 cagatcgctg gagtcaatca gaccacgcac gaagcgcaga tagttcggca tgaactgttc
    27301 tgcgtcgtcc atgatgaaca cacgctggac gtacagtttc aggccgtgtt tatgatcacg
    27361 gttccacata tcccacggag cctgggacgg gatgtacagc aggctggtgt actcctgctt
    27421 accttcaacg cggttgtggc tccaggtcag cggatcatta aagtcgtggg cgatgtgttt
    27481 gtagaactct ttgtactctt catcggtgat ttccgacttg ttacgagtcc acagcgcctg
    27541 cgctttgttg attttctccc aggagataac ggtttcgccg tctttctctt cgcgtttttc
    27601 gatctctacc ggcagcgcga tatggtcgga gtatttgctg atgatggaac gcacgcgcca
    27661 gtcatcgagg aactcgtctt cgccttcacg cagatgcagg gtgatttcag taccacgatc
    27721 ttctttggtg atgtcggcaa cggtatattc accttcgcca gccgattccc agaagacgcc
    27781 gttttctggt ttttcgcctg ccgcgcgagt acgcacggtc actttgtcgg ccacgataaa
    27841 cgcagagtag aaaccaacac caaactgacc gatcagctgg ctgtctttcg cctggtcaga
    27901 acccagggat tcgaggaatg atttggtacc ggatttagcg atagtcccca gatggtcaat
    27961 cacttcgtcg cgggtcatcc ccacgccgtt atcggagatg gtcagcgtgc gcttgtcttt
    28021 atcgaaagag acacgaacgc gcagttcgcc atcaccttcg tacaggtccg ggttagatag
    28081 cgcacggaaa cgcagcttgt ccgccgcatc ggaggcgtta gagataagct cacgcaggaa
    28141 gatttcttta ttggaataga gagaatggat catcaggtgc agaagctgtt tcacttctga
    28201 ctgaaaacca cgagtttctt gtcctttcat gtaggtctac ctcaataatg ccattttaag
    28261 gttaaaaaca ggatgaggga gagatgggga caagggagaa taatttcaag cgagagcagg
    28321 tgatcctgct ctcgtttgct taaaaacgaa tcttatgacg cccggcaagg gagtgtgaca
    28381 gcgtggtgcc atcgaccatt tccagttcgc cgccaaccgg tacgccatga gcgattcggc
    28441 tggcttccac gtcatattgc gcgcaaagct cggcaatgta gttagcggta gcttcacctt
    28501 caaccgtggg attggtggcg aggatcactt cagtgatttt ttcctctgcc agacgctgtt
    28561 ccagacgatc aaggccgata tcatccggac cgatgccgtc cagcggtgac aggtgcccca
    28621 tcaacacaaa ataacggcct gaaaactgcc ccgtctgctc aatggcgtag atgtccgccg
    28681 gactctccac cacacagatt tgaccgtttt cctgacgacg cggattcgaa cagatgttac
    28741 agacttcctg ttcggtgaaa gtgcggcaat cggcgcagtg gccgatttcc gacatcgccc
    28801 gggtgagcgc ctgcgccaga cgcatcccgc cgctacgatc gcgctgaagc agcgtgaacg
    28861 ccatacgctg cgccgacttc gggccaacgc ccggcagaca gcgcagtgct tccataagct
    28921 gtgttaacag cgggctggtt tgcatcagaa cggcatctta aagccaggcg gcagctgcat
    28981 tccggaggat acagaggcca ttttttcttt ctgcgtttct tcaatacgac gtgctgcgtc
    29041 gttgaatgct gcagccacca ggtcttccag catctctttg tcgtcttcca gcaggctcgg
    29101 gtcgatctct acgcgacggc agttgtgtgc accgttgatg gtcactttta ccagacctgc
    29161 gccagattcg ccggtgactt ccagctgcgc gatctcttcc tgcattttct gcattttttc
    29221 ttgcatctgc tgggcttgct tcatcaggtt acccagaccg cctttaccaa acataggttt
    29281 ctctctcaat cacgttaagg atgacgaacg taagctgtgc ttacgatcaa atggggcgga
    29341 tactttcttc atccagctcc gcatcgaaga accgacgcag ggtctgaata ttattatccg
    29401 caataatgga ctcgcgcgcc tgcgcaagtt tttcttcgta tatcgcctga cgccactcca
    29461 gcggcgtacg caccgcggga ttatcatctt caacgatagt cagttcaacc gttgaacctt
    29521 ttaacgtgct caacgcttca gccagttttt gctgtgcacc gcggttgttc aaatgccgct
    29581 gagaggagcg caaatgcaga catactgcgt tgtcgctctc ctctttccag gcatttaagg
    29641 ccacctgttc gaccagtttt ggtagcgaaa gttgactcac ctgtgccgcc cacgcgtcgc
    29701 gctcaatggc ttctgccgcc agcttcgccg ccagttccgg cgttttttca tgttccagcg
    29761 cttttttcag cgccttcggc gtggcgacca cttctttttg ctgcatcact ggagtggtag
    29821 ccttccagcg atacgcttct tttttggctg gcgctttttc cagcgccgat ggcaccggac
    29881 gcgcctgaac gcgatcggtg accgaagcca gtctttccag cgcagcgtta ttcaccggcc
    29941 gcgcgcgggt agcggctgcc ggttcactct tttttgcttt ggttgctccc tgcacgcgct
    30001 gcaactgctg gcgcgccgcc agcacctggc tggtggtttc cgggagcggt acagtcggtg
    30061 cctgctgcgg cgctgattgc ggttgcggcg gcacctgggt tggcgtcatt actgccgttg
    30121 gcgcgacggg cgcaaaggac tggcgtggca cttctggctc aggcagaggc atacgtggat
    30181 ggaacgccag cgcgcgcagc agcgtcatct caacgcccat gcggcggtcc ggcgcatacg
    30241 gtaattcttt gcgaccaatc aacagcgtct gatagtaaag ctgaatatcc gtcggcggta
    30301 tggtgcgcgc cagttcacgc atccgcagct caatggcggc catgtcgttg ccaagtgcag
    30361 caggcgaaag ttgtaccatc gcaatacgat gcaacaggcc gagcatttcc accagcaacg
    30421 cttcccactc gataccacgg gcagcggctt cattaatcag cgccattacg cgctcgccgt
    30481 tggcctcgac catcgcttca accagcgaca gcgcctgatc gtcgtcaagc gtacccagca
    30541 tcgcactgac tgcctgggtt gaaacctggc cgtcaccgct ggcaatcgcc tggtcggtca
    30601 gacttaaggc atctcgcagg ctgccttcag cggcgcgcgc cagcaattgc aacgcccgcg
    30661 gctcgtgagc gatatgttct tcgttgagga tgtgctcaag ttgatggcga atttgctcga
    30721 catccagcgc cttgagatga aattgcaaac agcgcgacaa aatcgtcacc ggcaatttct
    30781 gtggatcggt cgtcgccagc agaaacttaa cgtgctccgg cggctcttca agggttttta
    30841 acagtgcgtt aaagctgtgg cgcgacagca tatgcacttc gtcgatcaga taaactttga
    30901 aacgaccacg cgccggagcg tactggacgt tatccagcag gtcgcgggta tcttcaactt
    30961 tggtgcgcga ggcggcgtcg atttcaatca gatcgacaaa gcgcccctgc tcgatttcac
    31021 gacagttatc gcacacgccg cacggcgtcg cggtaatgcc ggtttcgcag tttagcccct
    31081 tcgccagcag tcgggcgata gaggtttttc cgacgccacg ggtaccggaa aaaagataag
    31141 catgatgaat acgccctaac gacaagccgt tcgccagtgc ggtcagcaca tgttcctggc
    31201 cgacgacgtc agcaaaggtt tgtgggcgcc attttcgggc taagacctga taactcattg
    31261 gcaggctctg aaacgctgga aggtggattc acgaaggggt aatgctaaca cagccccgtc
    31321 agaacggcga ggctgaatgc acatcttgtg ctgtccgtag cgtgggcagc acaagactgg
    31381 cgataattaa tggcccggga acgggacaag gctgtagctg gtaatgccct gtttttcgag
    31441 acgctgttcg ccgccgagat cgaacaggtt gataatgaac gcagcgtcag ccacttcacc
    31501 acccagacga cggatcagtt taacggtcgc ttcgatagtg ccgccggttg ccagcagatc
    31561 gtccaccacc agaactttgt cgcccggttt gatggcatca acgtggatct ccaactgatc
    31621 ggtgccgtat tccaggtcgt aagtttcact gatggtttca cgcggcagtt tgcccggttt
    31681 acggaccggt acaaagccaa cgcccagacc cagagctacc ggagcgccaa acaagaagcc
    31741 acgcgcttcg gtgccgacaa ctttggtaat gcccgcattt ttgtaacgct caaccagcaa
    31801 gtcgatgctg agagcgtaag ctttcgggtc ttccagtaag ctggtgacat cgcggaaaag
    31861 aatgccgggt tttgggtagt cctgaatgct tttgatgcta tttttgagat actcaagctg
    31921 ctgtgcagtc gcggtcataa gtgtatgcct gcttgttacg gtggtactca cggcgcgttt
    31981 taaacgtatc aaaagtaaca gttgtttaac ctttgaccgc ctgcgcctgt gctcgaaaac
    32041 gccagaatgt actggctgta cgcaataatt gcaactgcga ttgttgtgct tcagtgcttt
    32101 tcttgctttt catcaatcac cggaattcgc cacatataga aaagcaaaca ggcgagaatt
    32161 accagcaaca tgatgcgcac ccatggcatc tggacgaacc acagagaaat ggcaaacgtg
    32221 agcaaaataa gcaaaatcgc ccgcggtttg acgccgcgcg gcatcgcatg atgtttctgc
    32281 cagaaacgta gatagctgcc aaaccatgag cggtacagca accaggcgtg aaagcgcgag
    32341 gaagaacggg caaagcacca ggccgccagc aggataaacg gcgtcgtcgg taataccggt
    32401 aataccacgc ccagcgtacc cagcactacc gccagccagc caatgatgat taaaatgatt
    32461 cgttgcatat tgagtgttgt cagcttacag agtggctact ttagcataac aattatcatt
    32521 ttcattgagg tcttatcgtg aaaaccgccc tgctgctgga aaaactggaa gggcagctcg
    32581 ctacgctgcg tcagcgttgt gccccggtag cgcaatttgc cacgctaagt gcccgttttg
    32641 acagacacct ttttcaaacc cgtgcgacaa cactgcaggc ttgtctcgat gaggcgggcg
    32701 ataatctggc tgcgcttcgt catgcagttg agcagcaaca gctgccgcaa gtggcctggc
    32761 tggcggaaca tctggcggca caactggaag ccatcgcgcg tgaagcctcc gcctggtcat
    32821 tgcgcgagtg ggacagtgcg ccaccgaaaa ttgcccgctg gcagcgtaaa cgtattcagc
    32881 atcaggattt tgagcggcgg ctacgtgaga tggttgccga acgcagagcc cgcctggcgc
    32941 gggtgaccga tctcgtggaa cagcaaacgc tgcatcgtga agtggaagcc tatgaagcgc
    33001 gcctggcacg ctgccgccat gcgctggaaa aaatcgaaaa caggctagcg cgcttaaccc
    33061 gctagcaatg gagagagtat gtcactggaa aatgcccctg acgatgtcaa actggctgtc
    33121 gatttgattg tgctgctgga agaaaatcag atcccagccc gcaccgtgct gcgcgcgctg
    33181 gatattgtaa aacgcgatta tgaaaagaaa ttaacgcgcg atgatgaggc ggaaaagtga
    33241 gaaataaatg gggccgctaa atgtccccta agattgtggg caaacgcaaa actgcctgat
    33301 gcgctacgct tatcaggcct acaccgctcc tgcaatacat tgaattcttg cggtttcgta
    33361 ggccggataa ggcggtcacg ccgcatccgg catgaacaac gcgcactttg tcagtaatct
    33421 cggggcaact aaatgtcgcc ccctttggtt attaccctac cgctggcgtc gggtcatcgc
    33481 ctttgtagtc gcgtttgact tccgtcacct catcgccctt ctcgttgtgc agatgcactt
    33541 caagctggtt aaaggcaatg ttgatgtcgt tttcacggca cagctgatcg atagtacggt
    33601 tcagctcatc gacagtacga ctacggtcac gcagttcacg cacatacaga cgcagctcat
    33661 gatccaacgt gctggcacca aacgccgtaa agaagacttc cggcattggt tcgtgcatca
    33721 cccttgggtg ctcagtcgcc gccttcagta acactttacg cactttttcc agatcggagc
    33781 cataggccac gccgagacgg atcaccagac gcgtagtagt gtcagtcaac gaccagttga
    33841 tcagacgctc ggtaacaaac gctttgttcg ggatgatcac ttctttgcga tcgaaatcgg
    33901 taatcgttgt cgcacgaata cggatcttac ttaccgtccc cgagaagcta ccaatggtta
    33961 ccgtatcgcc aatacgcacc ggacgttcga acagaatgat caaaccggag acgaagttac
    34021 cgaaaatttc ttgtaaacca aaaccaagac ctacggataa tgctgcggcc agccactgga
    34081 gtttatccca agagacgccc agcgatccga acaccgtcat cgcaccaacg gcaataatga
    34141 tgtagttaag gatggtagta atggcatacg acgcgccctg acgcatattc agtcgcgaga
    34201 gcaccagcac ttccagtaaa ccgggcaggt tgcgaatcaa cgcccaggcc accattgagg
    34261 cgataatcgc aaacaacaga ctgcccatgg tgacgttttt caccaccgca gcgccagctt
    34321 cagtgccgtt gtaatgccag agcgtgatgc tgtcgagata gctgaacacg gtgatcaaat
    34381 cggaccaaat tgcccagaac atgacaccga acagcgcaaa catcagcaac atggtaatac
    34441 gcagcgtctg ctggttaacc tgttccagtg caatggtggg ttcttccggc ggttcggcac
    34501 cttctgcgcc ctctttcacc agattctgcc gacgcgccag cgcacgacgc caggcgatac
    34561 gccgcgccgc tacgcttaag ccgcgcaata ccgtctggta gagcaagttc cagatgatca
    34621 ccagataaac ggtttcaatc caacgcccgg agaggcgcag cgtggtgtag aaataacccg
    34681 tggcagtcag caccatcaat gcaatcggga taatcgacag cacggtaatg gtgaccagtc
    34741 gcatggtgtg cgactcttta tcacgccagc tttcgcggca cattggccat accaggaagg
    34801 caatcagcag caggttgaag aaaatcatcg cctgccccag cacatcatcc atcagatgca
    34861 gcggggaaag ttctgccacc acagaccaga aatggatagg cagcaacgcg agactgatgc
    34921 ggacaatttg ccgacgccag tggctggtct gctgctccgg catgccgaag tgacgcacgg
    34981 caacgccatt tttctccagt accttccagc acaggccaaa caccagccag aaaatcgcca
    35041 gttttttact gaacgaccat agcagttcgc tgatgttgag ctgcatggtc aacagaatca
    35101 ggccaaccgc gagaataatc aggcacaccg gcagcgcgcg gatcaggtcg ataaggatcg
    35161 cttttggtgt gttgagctgg ctatcgttac gcagggaacc cacagcggaa gccagtttct
    35221 gttgatacgc tttcagccag cccagacgcc agtggatcag cccggcaatc aacagcagcg
    35281 gcaaaccggc gaggaaagcg ataaaaacgg caggccaggc tttttcccag ttcaccgtga
    35341 ttttcatcga cttaaattca tctttcaggc tttgcgggaa cgctttgatc cagtcccagt
    35401 ccattggacg gttactgttc acccaaaaga tttgctgagt caggatggat ttcaggtttt
    35461 tcgatacgct cattaactgc tgctggttga tttgcaggtt aatggccatc atcagctggt
    35521 tacccaactg tttgttgagt tgatccagca attcgcgacg catatcaacc acttgcaata
    35581 acgcatcgtg aacttcgctg ttgacttcgt tggtgtgacc ttcttccagt ttgttgacga
    35641 acgcatcgct ctggaagagt gcatcacgct gctggttaac ttcaaactgt tcgagacgca
    35701 aatccgcgat gcggttggtc atgttttcca gttcatccgc cgagggcagc gtttgttgtt
    35761 gctggtaaag gatacgagac aacagcaggc tgcccttcag gacggcaatc tgctctttaa
    35821 tattgcgttc cgattgcagc gcccgctcca gccagttttt gactttaatg ttttgctgca
    35881 tcaactgatt accgttttca gtcgcggtaa tcagacgctg acttaactgc tggttaattt
    35941 ccagttcctg tttcaccagc ggattagcct gaatacgcgc ggcttcatcc ggggagacgg
    36001 cttcctgcgc cgttttttca gttaaagtca ggcgcttgct gtttaccgct tcttgcaaca
    36061 gctgcaactg gtgctccaga cgggcgctgt tcgccgtcac gtaatcacgt tgcttttgca
    36121 aggtatcctg caagacggtg ttcccttcca ggcttttacg ctgctggtca atctccgcat
    36181 tcagcaacgc ctgctgagcc tgcattaaca ctttctggct gggacgtaag gcagtctcgc
    36241 cgacatcagt cccatccaga cgactgcgaa tttgttgcag ctgctgcgaa gcgttataca
    36301 tcgcattttg cacgcgttcg ggctgcgtct gtaacgaaac cagctggctg ttataagacg
    36361 ccagatcgtt ttgtgcgttt tgcaaatcgt ccagcgcctg ggcaacgcga gtttccagct
    36421 ggcgcaacga aagcgtactc agaattttgc gcgtttcttc gtcgttatcg acatcgctaa
    36481 gtgctgttaa cgccgcggtc gcctggcgca ttttttccgg cgcttcagcg actttttgcc
    36541 gtagctgaac tgtctcttct tttatgcgat cgattttatc gagggtggct aatgtatctg
    36601 tcagatcctg ctgcaccagt ttgtcctgag cagaaagatc tttttgttta tttagtgagt
    36661 caagttgcgc ctgcaggtcc gcttttgtcg gcagatcacc attcgatgac gcccgcgcaa
    36721 acgccgtgtt ctggcataac aagacaaaaa caaaagcaat aaatgctgaa aaaacaaaat
    36781 gccgtgatcg tttgtaatac tggaacatag tcatgatgaa tgaaggtttc tgaacctgaa
    36841 gaacgacctg aaaaagtcaa accgcaagaa tatcacgacg cagtgaacca gaatagcaac
    36901 gacgaaaatg tccaggaaaa atcctggagt cagattcagg gttattcgtt agtggcagga
    36961 ttacgaagcg tggggcacag gagatacatc tccagtaaga tggcgacgta atcgcgggct
    37021 tcttttttaa gatcaaaaga ttgcggggca aagagccagt tttccatcag gccggaaata
    37081 tagccgcgca taataattgc tgcccgacgc gtcattaaat ccgcaggcaa cattttcgct
    37141 tcaatacaat gttttaacgt ttgttctata cggtcataac tttccagaca gagattacgt
    37201 tgtgcctgtt gcacaacagc catttctccg acaaattcgc atttgtggaa tataatctcc
    37261 atcaataatc gacgccgttc ttctgtcacc gtggattcaa gaacatgaat taatatctct
    37321 cttaatactg agagtggatc gccagggaat tttgcctgat actcaagctc tagttcacca
    37381 atattggatt ctgacagttc ccagatctca ctgaacaaat ccgacttgtc tttaaaatgc
    37441 cagtagattg caccgcgcgt aacgccagct gcttttgcaa tctcgcccag cgaggtggat
    37501 gataccccct gctgtgagaa aagacgtaga gccacatcga ggatgtgttg gcgcgtttct
    37561 tgcgcttctt gtttggtttt tcgtgccata tgttcgtgaa tttacaggcg ttagatttac
    37621 atacatttgt gaatgtatgt accatagcac gacgataata taaacgcagc aatgggttta
    37681 ttaacttttg accattgacc aatttgaaat cggacactcg aggtttacat atgaacaaaa
    37741 acagagggtt tacgcctctg gcggtcgttc tgatgctctc aggcagctta gccctaacag
    37801 gatgtgacga caaacaggcc caacaaggtg gccagcagat gcccgccgtt ggcgtagtaa
    37861 cagtcaaaac tgaacctctg cagatcacaa ccgagcttcc gggtcgcacc agtgcctacc
    37921 ggatcgcaga agttcgtcct caagttagcg ggattatcct gaagcgtaat ttcaaagaag
    37981 gtagcgacat cgaagctggt gtctctctct atcagattga tcctgcgacc tatcaggcga
    38041 catacgacag tgcgaaaggt gatctggcga aagcccaggc tgcagccaat atcgcgcaat
    38101 tgacggtgaa tcgttatcag aaactgctcg gtactcagta catcagtaag caagagtacg
    38161 atcaggctct ggctgatgcg caacaggcga atgctgcggt aactgcggcg aaagctgccg
    38221 ttgaaactgc gcggatcaat ctggcttaca ccaaagtcac ctctccgatt agcggtcgca
    38281 ttggtaagtc gaacgtgacg gaaggcgcat tggtacagaa cggtcaggcg actgcgctgg
    38341 caaccgtgca gcaacttgat ccgatctacg ttgatgtgac ccagtccagc aacgacttcc
    38401 tgcgcctgaa acaggaactg gcgaatggca cgctgaaaca agagaacggc aaagccaaag
    38461 tgtcgctgat caccagtgac ggcattaagt tcccgcagga cggtacgctg gaattctctg
    38521 acgttaccgt tgatcagacc actgggtcta tcaccctacg cgctatcttc ccgaacccgg
    38581 atcacactct gctgccgggt atgttcgtgc gcgcacgtct ggaagaaggg cttaatccaa
    38641 acgctatttt agtcccgcaa cagggcgtaa cccgtacgcc gcgtggtgat gccaccgtac
    38701 tggtagttgg cgcggatgac aaagtggaaa cccgtccgat cgttgcaagc caggctattg
    38761 gcgataagtg gctggtgaca gaaggtctga aagcaggcga tcgcgtagta ataagtgggc
    38821 tgcagaaagt gcgtcctggt gtccaggtaa aagcacaaga agttaccgct gataataacc
    38881 agcaagccgc aagcggtgct cagcctgaac agtccaagtc ttaacttaaa caggagccgt
    38941 taagacatgc ctaatttctt tatcgatcgc ccgatttttg cgtgggtgat cgccattatc
    39001 atcatgttgg caggggggct ggcgatcctc aaactgccgg tggcgcaata tcctacgatt
    39061 gcaccgccgg cagtaacgat ctccgcctcc taccccggcg ctgatgcgaa aacagtgcag
    39121 gacacggtga cacaggttat cgaacagaat atgaacggta tcgataacct gatgtacatg
    39181 tcctctaaca gtgactccac gggtaccgtg cagatcaccc tgacctttga gtctggtact
    39241 gatgcggaca tcgcgcaggt tcaggtgcag aacaaactgc agctggcgat gccgttgctg
    39301 ccgcaagaag ttcagcagca aggggtgagc gttgagaaat catccagcag cttcctgatg
    39361 gttgtcggcg ttatcaacac cgatggcacc atgacgcagg aggatatctc tgactacgta
    39421 gcggcgaata tgaaagatgc catcagccgt acgtcgggcg tgggtgatgt tcagttgttc
    39481 ggttcacagt acgcgatgcg tatctggatg aacccgaatg agctgaacaa attccagcta
    39541 acgccggttg atgtcattac cgccatcaaa gcgcagaacg cccaggttgc ggcgggtcag
    39601 ctcggtggta cgccgccggt gaaaggccaa cagcttaacg cctctattat tgctcagacg
    39661 cgtctgacct ctactgaaga gttcggcaaa atcctgctga aagtgaatca agatggttcc
    39721 cgcgtactgc tgcgtgatgt ggcgaagatt gagctgggtg gtgagaacta cgacatcatc
    39781 gcagagttta acggccaacc ggcttccggt ctggggatca agctggcgac cggtgcaaac
    39841 gcgctggata ccgctgcggc aatccgtgct gaactggcga agatggaacc gttcttcccg
    39901 tcgggtctga aaattgttta cccgtacgac accacgccgt tcgtgaaaat ctctattcac
    39961 gaagtggtta aaacgctggt cgaagcgatc atcctcgtgt tcctggttat gtatctgttc
    40021 ctgcagaact tccgcgcgac gttgattccg accattgccg taccggtggt attgctcggg
    40081 acctttgccg tccttgccgc ctttggcttc tcgataaaca cgctaacaat gttcgggatg
    40141 gtgctcgcta tcggcttgtt ggtggatgac gctatcgttg tggtagaaaa cgtggagcgt
    40201 gttatggcgg aagaaggttt gccgccaaaa gaagctaccc gtaagtcgat ggggcagatt
    40261 cagggcgctc tggtcggtat cgcgatggta ctgtcggcgg tattcgtacc gatggccttc
    40321 tttggcggtt ctactggtgc tatctatcgt cagttctcta ttaccattgt ttcagcaatg
    40381 gcgctgtcgg tactggtagc gttgatcctg actccggctc tttgtgccac catgctgaaa
    40441 ccgattgcca aaggcgatca cggggaaggt aaaaaaggct tcttcggctg gtttaaccgc
    40501 atgttcgaga agagcacgca ccactacacc gacagcgtag gcggtattct gcgcagtacg
    40561 gggcgttacc tggtgctgta tctgatcatc gtggtcggca tggcctatct gttcgtgcgt
    40621 ctgccaagct ccttcttgcc agatgaggac cagggcgtat ttatgaccat ggttcagctg
    40681 ccagcaggtg caacgcagga acgtacgcag aaagtgctca atgaggtaac gcattactat
    40741 ctgaccaaag aaaagaacaa cgttgagtcg gtgttcgccg ttaacggctt cggctttgcg
    40801 ggacgtggtc agaataccgg tattgcgttc gtttccttga aggactgggc cgatcgtccg
    40861 ggcgaagaaa acaaagttga agcgattacc atgcgtgcaa cacgcgcttt ctcgcaaatc
    40921 aaagatgcga tggttttcgc ctttaacctg cccgcaatcg tggaactggg tactgcaacc
    40981 ggctttgact ttgagctgat tgaccaggct ggccttggtc acgaaaaact gactcaggcg
    41041 cgtaaccagc tgcttgcaga agcagcgaag caccctgata tgctgaccag cgtacgtcca
    41101 aacggtctgg aagatacccc gcagtttaag attgatatcg accaggaaaa agcgcaggcg
    41161 ctgggtgttt ctatcaacga cattaacacc actctgggcg ctgcatgggg cggtagctat
    41221 gtgaacgact ttatcgaccg cggtcgtgtg aagaaagttt acgtcatgtc agaagcgaaa
    41281 taccgtatgc tgccggatga tatcggcgac tggtatgttc gtgctgctga tggtcagatg
    41341 gtgccgttct cggcattctc ctcttctcgt tgggagtacg gttcgccgcg tctggaacgt
    41401 tacaatggcc tgccatccat ggaaatctta ggccaggcgg caccgggtaa aagtaccggt
    41461 gaagcaatgg agctaatgga acaactggcg agcaaactgc ctaccggtgt tggctatgac
    41521 tggacgggga tgtcctatca ggaacgcctc tccggcaacc aggcaccttc actgtacgcg
    41581 atttcgttga ttgtcgtatt cctgtgtctg gcggcgctgt acgagagctg gtcgattccg
    41641 ttctccgtta tgctggtcgt tccgctgggg gttatcggtg cgttgctggc tgccaccttc
    41701 cgtggcctga ccaatgacgt ttacttccag gtaggcctgc tcacaaccat tgggttgtcg
    41761 gcgaagaacg cgatacttat cgtcgaattc gccaaagact tgatggataa agaaggtaaa
    41821 ggtctgattg aagcgacgct tgatgcagtg cggatgcgtt tacgtccgat cctgatgacc
    41881 tcgctggcgt ttatcctcgg cgttatgccg ctggttatca gtactggtgc tggttccggc
    41941 gcgcagaacg cagtaggtac cggtgtaatg ggcgggatgg tgaccgcaac ggtactggca
    42001 atcttcttcg ttccggtatt ctttgttgtg gttcgccgcc gctttagccg caagaatgaa
    42061 gatatcgagc acagccatac tgtcgatcat cattgataca acgtgtaatc actaaggccg
    42121 cgcaagcggc cttttttatg cataacctac gaacattaag gagtaattga accaccaact
    42181 caggatctca tacgaaaacc agtattaacc acggataaaa ttcataaaaa atactgattg
    42241 ttagttaatt tatattaagt agcgctaata gatttaataa tccataatca tttagaggct
    42301 attcttaatt atttgcggta attctttatt cattcctcgg ttattacgtc atattcagag
    42361 caatcctggt attagtgtca ccaatttcat ctggcgataa tcctgaaatg ttatgaacag
    42421 ttcgagcaaa ctgcttttac ctgctgcggg ttagtgctag tatgaaaaag tgagtcctgt
    42481 cccgattcct tcctaattgt aatttttcgt aataatgcga tgaaaacctg caaagagtgg
    42541 cttatagtta agctaacaaa cgagagggca agtccaggtc agtaagtttt ttccatcccg
    42601 aaaggtgtcc gttagttcaa ccactaagaa ggggacgcgt tatggatgaa tactcaccca
    42661 aaagacatga tatcgcacag cttaagtttc tctgtgaaac cctgtatcat gactgtcttg
    42721 caaaccttga agaaagcaat catggctggg ttaacgaccc aacctcggcg atcaacctcc
    42781 agttgaatga actgattgag catattgcga ccttcgcact taattacaaa attaagtata
    42841 atgaagacaa taagctcatt gagcagatcg acgaatatct ggatgacacc tttatgttgt
    42901 tcagcagtta tggtattaat atgcaggatc ttcagaaatg gcggaagtca ggtaatcgac
    42961 tattccgttg ttttgtcaat gcgacgaaag agaatcctgc gagtttatct tgttagaatt
    43021 attacaacca tgggtagaag tatgtccgaa aaacctttaa cgaaaaccga ttatttaatg
    43081 cgtttacgtc gttgccagac aattgacacg ctggagcgtg ttatcgagaa aaataaatac
    43141 gaattatcag ataatgaact ggcggtattt tactcagccg cagatcaccg cctcgccgaa
    43201 ttgaccatga ataaactata cgacaagatc ccttcctcag tatggaaatt tattcgctaa
    43261 taaataattc gctttcggag ctataaccgg ctgtttatta agaattttat actttttcac
    43321 catgaagaca taccctatgt gatctttatc acacagatgt aatgggaacg ttctcttcac
    43381 tgacttttcg tcttactgtg ttgccgcatt ttcagcaacc ggaggtcagt aatgagcaca
    43441 gaaaaagaaa agatgattgc tggtgagttg tatcgctcgg cagatgagac gttatctcgc
    43501 gatcgcctgc gcgctcgtca gcttattcac cgatacaatc attccctggc ggaagagcac
    43561 acattacgcc agcaaattct cgctgatcta ttcggtcagg tgacagaggc ttatattgag
    43621 ccaacgtttc gctgtgacta tggctataac atttttctcg gtaataattt tttcgccaac
    43681 ttcgattgcg tgatgcttga tgtctgccct attcgcatcg gtgataactg tatgttggca
    43741 ccaggcgttc atatctacac ggcaacacat cccatcgccc ctgtagcacg taatagcggt
    43801 gctgaactgg ggaaacccgt caccatcggt aataacgtct ggattggcgg acgcgcggtc
    43861 attaaccctg gtgtgaccat tggtgataac gtcgtggtag cctcaggtgc agttgtcaca
    43921 aaagatgtcc cggacaacgt tgtcgtgggc ggtaatccag ccagaataat taaaaaattg
    43981 taatcggttt ttcgcaactg ttatgcaaaa ttgtggtaaa tctgttactt cccctctact
    44041 attcccacgt taaaataggg tgttccctgg aaagttgcag ataccacgaa ggcaaacgat
    44101 gaccgaaata caacgcctgc tgaccgaaac gattgagtct ctgaataccc gcgaaaaacg
    44161 cgacaacaaa ccccgcttta gtatcagttt tatccgtaaa catccggggc tgtttatcgg
    44221 tatgtacgtt gctttttttg ccaccctggc ggtgatgttg cagtccgaaa cgctgtcagg
    44281 ctctgtctgg ctactggttg tattatttat cctgcttaat ggtttcttct ttttcgatgt
    44341 ctacccacgc taccgctatg aagatatcga cgtgctggat ttccgcgttt gctataacgg
    44401 cgaatggtac aacacgcgct ttgtacctgc cgcgctggtt gaagccatct tgaactctcc
    44461 gcgtgtcgcg gatgttcata aggaacaact gcaaaaaatg atcgtccgta aaggtgaact
    44521 gtctttttac gatattttta ccctcgctcg cgccgaatca acatcttaag ttagggttac
    44581 ataccaggcg taaagctctg cgcctggtca aatgacaatg atcgtttcca cccatcactt
    44641 catgaaatac cagctctacc tccttatctc ctgccagcct ttttccacaa tcagatatac
    44701 tttccctaca ctgtgttaat aaggatatgc tggtgagaac acgacatctg gtcggcctta
    44761 tttcgggagt actgattctt tcagtattgc tgcctgtcgg cttaagcatc tggctggccc
    44821 atcagcaggt agaaacatcg tttattgaag agctggatac ctattcctcc cgcgtcgcta
    44881 ttcgggccaa taaggtggcg acacaaggga aagatgcgct gcaagagctg gaaagatggc
    44941 aaggcgctgc ctgtagcgaa gcccatctca tggaaatgcg tcgggtatct tacagttatc
    45001 gctatattca ggaagtggtt tatatcgata acaacgttcc ccagtgttcg tctctggagc
    45061 atgaaagtcc gcccgatacc ttccccgagc caggtaaaat ttcgaaagat ggttatcgtg
    45121 tctggttaac atcgcataac gatttaggca ttatccgtta catggtcgcc atgggaacgg
    45181 cacattatgt cgtcatgatc gaccccgctt cctttattga tgtcattccc tatagctcat
    45241 ggcaaattga tgccgccatt attggcaatg cccataacgt tgtcataacc agcagcgatg
    45301 aaattgctca gggaattatt accaggctac aaaaaacacc cggtgagcat atcgaaaata
    45361 atggaatcat ttacgatatc ctgcccttcc cggagatgaa tatttcgatc atcacctggg
    45421 cttcaacgaa aatgttgcag aaaggctggc atcggcaagt ctttatttgg ttaccgctcg
    45481 ggttggtgat tggcctgctg gcagcgatgt ttgtgctgcg tattttgcgc cgtatccagt
    45541 caccgcatca tcggctgcag gatgctatcg aaaatcgtga tatttgcgtg cactatcagc
    45601 cgattgtctc cttagccaat ggcaaaattg tcggtgctga ggcactggcg cgctggccgc
    45661 agacagacgg tagttggttg tcaccagata gttttattcc gctggcacag caaacgggcc
    45721 tttctgagcc attgacgcta ctgattataa gaagcgtctt tgaagatatg ggcgactggc
    45781 tgcgtcagca tccacagcag catatttcga tcaatcttga atccaccgtg ctcacctcgg
    45841 aaaaaatccc gcaattgctg cgtgaaatga tcaatcacta tcaggttaat cccagacaga
    45901 tcgcgcttga actcactgaa cgcgagtttg ccgatccgaa aaccagcgcc ccgataattt
    45961 ctcgctaccg ggaggcgggc catgaaattt atcttgatga ttttggtacg ggatattcaa
    46021 gtttaagtta tttacaggat ctggatgtcg acattctgaa gatcgataaa tctttcgttg
    46081 atgcgctgga atataaaaat gtcacgccgc atatcatcga aatggcaaaa acactgaaac
    46141 tgaaaatggt agcggaggga atcgaaacca gtaaacaaga agagtggcta cgccagcatg
    46201 gcgtgcacta cggtcagggc tggctctaca gcaaggcatt accgaaagaa gatttcttac
    46261 gctgggccga gcaacatttg tgaatttttg ggctgacggc acgccgcaca gcccgtcacg
    46321 ctacctattc gtcgatgatt gactcgaatc cgccatagat cattcgcttg ccgtcaaacg
    46381 gcatggactc gccgaactct ttcatccgtg ggtccgacat catcttttga gtagcagcgt
    46441 cgcggacctc ttttgaaggg tattcaatcc agctaaagac aacctcttca ttctcttccg
    46501 ctttcaccgc catacgaaaa tcggtcactt tgccatccgg tacatcgctg gcccagcatt
    46561 cgacaatacg aagcgcgcca aactctttaa acaatggcgc agccttagcg gccatttccc
    46621 gataggcatc ctttttatcg gcaggaacgg caaccacaaa accatcaaca tacttcatca
    46681 gacatacctc cgaagcgtac tgcgcagcca gacaattctt gcgacgtcag ttgtggttaa
    46741 agtttagcca tgttggggtt ttttgacgaa acggtgggag gcagaaatgg gtgggggccc
    46801 tgccagctac atcccggcac acgcgtcatc tgccgtggct gcttccttcc ggacctgacc
    46861 tggtaaacag agtagcgttg cgggagaacc aacagagccc ccattgagag cgttgagaac
    46921 caacgcgcag gcgcattatc actgctgccc cggctaattg caacccatcg cgtgccagat
    46981 gctggtttct tgcgcaatgc gacttcactc gggcgttttt ccagattatg ctgaaaaatt
    47041 acctcaggaa gaaaagatgg aaaaagaaga ttcatttccc caacgcgtct ggcaaatcgt
    47101 cgccgctatt cccgaaggct atgtcaccac ttacggtgat gtggcgaaac tggcgggatc
    47161 gccccgcgcc gcgcgccagg tgggcggtgt gttaaagcgt ctccctgaag gcagcacctt
    47221 accctggcac cgggtggtta atcgccacgg cacaatttcg ctaaccggac cggatttaca
    47281 gcgtcagcga caggcattac tggcagaagg tgtgatggta tcgggaagcg ggcaaatcga
    47341 cttgcagcgt tatcgctgga actactaacc ctctccgggc ggagagggtt aacgtcgctt
    47401 aatattgcgt aggagctgga actgccgaag acggattcac ctgagttggt gaagtcgaag
    47461 gtacggtagt cgttgcacca ccgctggcct gaaccggcac ggcggtttgc tgcaccggca
    47521 ccaatgtcag gtcggcttta gttccgccct tgttgatcac cggctgaacg gtatcggtaa
    47581 taaataccag tttgtcattc acggtaatcg ccgcactcaa cagaatacgc gcgttcggct
    47641 gaacatctgc cgggttaaat ggcagaacaa agctgaatgg tgactgttta ccttcagtac
    47701 gcaccgcttt ctgcgccagc acttttgacg gtgcatcggc taacgacgcg tcagaaagtg
    47761 tcacggtcag cacagcatca ggcggcagtg cgactttctg acggatccag acggtaccgg
    47821 agacattcgg ttgctggata gcaggttgtt gtgttgctga aatagacgta tttgcagccg
    47881 gggctggcgt ctgaatatcc gcgcttttat ctgcgcaagc cgccaacgca atcgcaaccg
    47941 ctaaaccact ggccatgtgc acgagtttca ttcatttctc cttattatca atgcaccagc
    48001 gggctaactt tcctcgccgg aagagtggtt aacaaaatag taacgtcaac aagtgtggca
    48061 cacatcacgc atttctgcct gtaattagcc cgtaattcag accattgcac ccatcggacc
    48121 agtagcaaat ttgcgttata ctcaactcac tttggcttgc tgcggcagct ttgttactgg
    48181 agagttatat gagtcaggcg ctaaaaaatt tactgacatt gttaaatctg gaaaaaattg
    48241 aggaaggact ctttcgcggc cagagtgaag atttaggttt acgccaggtg tttggcggcc
    48301 aggtcgtggg tcaggccttg tatgctgcaa aagagaccgt ccctgaagag cggctggtac
    48361 attcgtttca cagctacttt cttcgccctg gcgatagtaa gaagccgatt atttatgatg
    48421 tcgaaacgct gcgtgacggt aacagcttca gcgcccgccg ggttgctgct attcaaaacg
    48481 gcaaaccgat tttttatatg acggcctctt tccaggcacc agaagcgggt ttcgaacatc
    48541 aaaaaacaat gccgtccgcg ccagcgcctg atggcctgcc ttcggaaacg caaatcgccc
    48601 aatcgctggc gcacctgctg ccgccagtgc ttaaagataa attcatctgc gatcgtccgc
    48661 tggaagtccg tccggtggag tttcataacc cactgaaagg tcacgtcgca gaaccacatc
    48721 gtcaggtgtg gatccgcgca aatggtagcg tgccggatga cctgcgcgtt catcagtatc
    48781 tgctcggtta cgcttctgat cttaacttcc tgccggtagc tctacagccg cacggcatcg
    48841 gttttctcga accggggatt cagattgcca ccattgacca ttccatgtgg ttccatcgcc
    48901 cgtttaattt gaatgaatgg ctgctgtata gcgtggagag cacctcggcg tccagcgcac
    48961 gcggctttgt gcgcggtgag ttttataccc aggacggcgt gctggttgcc tcgaccgttc
    49021 aggaaggggt gatgcgtaat cacaattaaa aaaatagccg gaggtaaaac cgtccggcta
    49081 ttttttgcag tgcttgttac gcgttatagg cattctcgcc gtgactgttg acatccagcc
    49141 cttctcgctc ctgctcttcc ggtacacgca gaccaaccgt cagatccgcc agtttgtagc
    49201 caataaatgc caccacaccg gaccagacga tcgtaatggc gatgctttcc agctgtacca
    49261 gcaactgatg gcccatcgtc acaccttcag cgaagcccac gccacccagc gaactggcgg
    49321 caaaaatccc agtcatgata cagccgacaa tgccacaaac gccgtgcaca ccgaagacat
    49381 cgcagggatc atccacccgc agcaagcgtt tgagcatagt aacgccccac aagcccgcca
    49441 gaccagctac cacgccgata atcaacgcgc cgccaacccc aatgtagcca cacgctggcg
    49501 tcacgccgac cagaccggca atcgcgccag aacacgctcc cagcagagaa ggcttaccac
    49561 gcagcgccca ttcaccgaag atccagccaa gaattgccgc cgccgttgcg accacagtat
    49621 tcacaaatgc cagtgccgcg atttcattcg ccgtgcccgc tgacccggcg ttaaagccaa
    49681 accaaccaat atagagaatg gcagtaccgg tgaagaccat tggcaggttg tgcggtttaa
    49741 acgcctcttt gccgaagccc acgcgtttgc ctatcagata cgcgcccacc agaccggcga
    49801 ttgcggcgtt aatatgcacc accgtgccac ccgcgaaatc cagcgcaccg tgagaagcca
    49861 gcaaaccacc gccccacacc atatgcgcaa tcggaatgta agagagcgtc agccatacca
    49921 ccacgaaaat caacacagct gagaagcgga ttcgttccgc cagcgcccca acgatcaagc
    49981 cgacggtaat gcaggcaaac gagccctgaa acgccacgtg gatatactga taaatgctgc
    50041 ccatcaccgc cgtcagttcg atgtttttta gcatcaacca gttaatgttg ccgaagaagt
    50101 tgttgccctc accaaacgcc agcgagtaac cgtaaaccac ccagagaata cagaccagtg
    50161 caaacgtcac cgtcacctgc gtcagcatcg acagcacgtt tttgccgcga atcaacccac
    50221 cgtaaaacag ggcaatcccc ggaatagtca taaacagcac cagcgcagta caaatcatca
    50281 taaacgcatt gtcggcttta tcggccaccg caggtgcagc cattaccagt cccggcagca
    50341 tcgccagtga agcaagccca gtttttatcg tcgctatctt catttttcgt tcctgttgct
    50401 gtgtgccaga gattacagcg ccgcttcgtc ggcttcgccg gtacgaatac gaatgacgcg
    50461 ttgcaattca gcgacgaaga ttttgccgtc gccaattttt ccggtgtaag ccgccttact
    50521 gacgatatcg atcacttcat cgagttggtc atcagcaatc gccacatcaa tttttacttt
    50581 tggcaggaag ttgacgctgt attccgcccc ccggtacagc tcggcatgcc ctttctgacg
    50641 cccgaaacct ttcacttcgg tgacggtcag gccctgaata ccaatggaag ataacgcttc
    50701 acgaacgtct tccagcttga atggtttgat tatcacggtc accagcttca tagatcccct
    50761 ccggtcagaa ttcggtaatg gttctgctac acgaagaagg tattgcaagc ggtgtgccag
    50821 aaatgaaaaa cgagaaagag agcaggaagt taagaagatt gaaaatgaaa acgcactatg
    50881 acagtgcaca gtgcgttaca tttttgcacc aacgatgaaa tattgcgcta ttcaggcgct
    50941 caatgattct tcttcacgca cgctggctgc cagctcttcg cccgcaagtt gcagttgata
    51001 catctgccag tagcgtccct gggccgccag cagttgctgg tgagtgccct gctccacggc
    51061 ttgcccacga tgaagcacca gaatggtgtc ggcatcaaca atggtcgata agcggtgagc
    51121 aatcaccacc agcgtggtat gttcacgcac agccgccaga gcatgttgaa tcgcctgttc
    51181 agtaccggag tcaatgctgg cggttgcctc atcaaggatc aggatttgcg gcgtctccac
    51241 caacacgcgt gccagtgcca gcagttgctt ttgcccgacg gagagattat tcccctgctc
    51301 gcccagcggc gtataaatac catcactcat gctgcgcgcc agctccgcca gttgcacggt
    51361 ttccagcgcc tgccagacgc gttcttcgga gatatcacgc cccagcgtca cgttggcgag
    51421 gaaggtatcc gccagcacca ccggatcttg ctgcaccatt gccacgccct ggcgcagcgc
    51481 gctgtgactt agcgaactta atggacgacc atcaaggcga atctcacctt ccgttagcgg
    51541 gtaatagccc atcaataaac tggcgagggt gcttttgcca ctgccggtat gcccgaccag
    51601 tgccacaaag ttacgcgaag gcacagagag attaatgttc tttagcacca gattgtcatc
    51661 gcgataagca aatgacacgt tatcgacttc gatggtgcca ctctgtaacg ggcgatcatc
    51721 attgccatat tgctggcgcg gtccgtccat cagttcaaac acgcgctcac cagcaacaac
    51781 agcctgttgc agcatcgcct gttgcgtggt cagttcgatt aatggttcgt taagtcgccc
    51841 aagatagctg ataaacgcat acagcacgcc cacttcaatg gtgccgctag cggagaagcc
    51901 aaacagcatc aacaagccac aaagaatgag cgatgaaaac agactcagca gcggacgcag
    51961 cagaaaaccg tcgaggcgca gggtttgcat cctggccata tagtgtgaac gactggcctc
    52021 ccccatacgt tcgccaaatc gcgcctgctg acggaactgc tggataacgc tcatgccatt
    52081 gatgatttcg ttaaagccgt cgttgatatc cgctagatag gcgcgcacac gacggacaat
    52141 cggcgtgctg taacgctggt atatcaccat taccaccagc accaccggga aaatcattat
    52201 cgccaccagc gccattcgcc agtcgaggct gaacatcgcc accagcatcg cgcccaccag
    52261 tgcggcgcta cgcaggacag ttgccactac ggtaacgtag agatcgcgga tcacttcagt
    52321 gtcattagtg acgcgggaaa tcacctgccc gacgggttgg gtatcaaact cgcttaatgg
    52381 ctggcgcaat gcggcatcca tcacgtcggt acgcaactgt tgcactacgc ctactgccgc
    52441 ccgattaaac agcagcgact gcgcgtaatg tagcccggcg gcaaacagtt gtagcccaac
    52501 atacgccgca gccagccctg caaccatttt caacggcagg ttatttttcg ctaccatatt
    52561 gtcgataaaa tagctgataa gcagcggccc actgacttct gccgctgccg caacccacat
    52621 catcaggacc gcaatcccca gcggtttacg ccacggcgaa ccgtacgcta acagacgctt
    52681 gagagtcggc cacagttggc taaaactacg catcgacggc ctcctcgcga atttccggag
    52741 cgtcgtcgag cgccgcctcc agttgttgat agcgatacat atcgcgatac cagccgcttt
    52801 gttgcgccag ctcatcatga ttgccacgct gggcgatatg tccgtgctgc atcacaataa
    52861 tttcactggc ttccgtcagt gccgaaaggc gatgggcact gatgattacc gttcttccct
    52921 gtccccactg acgcaggtta tgcaggatct ggtgctctgt gcgtccgtcc accgccgaaa
    52981 gcgcatcatc aaggatgagg atttccgcgt tgactaataa cgctcgagca atggagatac
    53041 gctgtttttg cccaccggaa agcatcacgc cgcgctcgcc cacttctgta tcgtaacctt
    53101 gcggtagacg caaaatatcg tcatgtacgc tggctaaccg cgcgacatgc tcaatctctt
    53161 gctgggtggc attcgggcaa cccaacgcga tgttgttcgc tacggtgtcg gaaaaaagga
    53221 acggtgtctg gctaactacc gccaggcggc tacgccagct atcgagttgt aacttcgtca
    53281 gaggaatatc atgaaagcga atatccccct cgctgacgtc gaaatgacgc tgaatgagcg
    53341 acaacagggt acttttgccg gaaccagtcg gcccgcagat acccagcatc tgaccgggtt
    53401 tcagggcgaa attgacgttt tccagcgcag gatggtcagt ctgcggatac gtgaactggt
    53461 gaatatttac atcaagttcg ccacgccctt ccggcacggg ttcgctacca tcgttcacca
    53521 ccggcgcttc cgccagcatc gcacgaatac ggctgtacgc agcactacca cgttccacaa
    53581 tgttaaacat ccatgccagc gccagcattg gccaaatcat cagacctaaa tacatcataa
    53641 aactggtgag ctggcccagc gttaaactgc cctgcgccac catccaacta ccgccgccaa
    53701 tcgccagcaa gttcgccata ccaatcgcga tatagatggt tgggtcgaaa cgagcatcaa
    53761 tacgcgccac ccgcatgttt tttttgccgg tatcttcggc atccgcggca aataacgccg
    53821 actggcgatc ttccagacca aaggctttga tcatgcggat actggtgagg ctttcctggg
    53881 tgcggtcatt aagactagaa aacgccgcct gtgccagctt aaagcgttca tgcaaggcat
    53941 cgccgttgcg cttgatcatg atcgccatca ctggcatcgg caacagggca aataaggtca
    54001 actgccagct aatttgcgta gacatcataa tcaacacagc gcagcccatc accagtgaat
    54061 ccaccagcgt cagcacccct tctccggcgg caaacacgac gcgatcgacg tcattggtcg
    54121 cacgagccat gagatcaccc gtgcgatgac gcaggtaaaa ctcaggatgc tgccggctta
    54181 gctgacggta ataatcttca cgcagttcaa cagccagttg ataagacgca ccaaacagca
    54241 atacccgcca gacgtaacgc aggagataaa ccacaacggc aatcagcacc atggtggcga
    54301 tccacatcag gatctgcccg gtagtaaagt gttgttctgt cacgccatcg acaacaatac
    54361 caaccacttt tggcggaacc agttgcagca tcgcgataat gacaagcaag gcgacagccc
    54421 cgagatagcg acgccattcc cgacggaaat accagcttaa ttgagcaaat aatcgcacgc
    54481 gttgtgtcct gacctgattc tggatattta ttcgatgggt aaagaagtgg tgtatttaat
    54541 ctgttccatc gcgaagctgg aagtgacgtc cgacagcccc ggcacgctgt ttaccagacg
    54601 cttataaaac tcgtcgtagc gtttcatgtc ggcaacctgg acgcgcatca gataatcgta
    54661 ttcaccagcc atgcgccaga accccagcac ttctggcatt tcggtaacca ccgtgacaaa
    54721 gcggcaatac cattcgctgc tgtgatgttg cgtttttatc agcacaaagg cggtcaggcc
    54781 gaggcctatt ttttccggat ccagcagggc gactttgccg ataaggatac cgtcgtcctc
    54841 cagccgtttc aggcgcttcc agcaaggggt ggttgtcaga ttaacggctt cagccagtgc
    54901 ctgcaaagag agggtgcaat cctgctgcag taaggccagc agcttacggt caattttatc
    54961 taacatagcc cttccacgga gaattttttt ctcttttaat tctattttaa aggtgaaatc
    55021 gcgacaattt attctgtgcg gtttaaacac cacggcacaa aatgacaaat tcaacgcaaa
    55081 aaaaaaccgg gcaattgccc ggtgctggaa gggatctcgt tattcggggg aataaggtag
    55141 atgtggatag tccagccagt gcgtcaaata gtgagagaca gcctgatttc ggcaatgtcc
    55201 aatcaccggt aaatgcggga gctccgcgcg cagttgcggc atcgcattgc ccataataaa
    55261 cccgctaccg acgctgccta acatttcgcg atcgttcatc gcatcaccaa aggccatgca
    55321 atcgcgcaac gataaaccta aatgttgggt cagcaccgtc aatgcagcgc ctttattgca
    55381 gcccaccggc agcacttcga ggcaatccgt ggcggaaaaa cacaaatgtg cacgctcgcc
    55441 taatgcttcg tatagctgga tctgcaagcg tgtaagatcg tcgtgatcgc cacagaagca
    55501 gatcttggtg acgctgccga gtggcatttt tttgacatcg attatctgat aacgaaaacc
    55561 gctatagaca aatgcctgca acaacgcagg gatctctttc ccggtaaacc aaccgtcgtc
    55621 attgaagata tgcatgctgg ctcgggtatc ccattgctga tacagcacca gctccgcgac
    55681 atccgcaggt aaatcatcac gatgtaaaag ttcaccttcc agagaatgca cgcgcgttcc
    55741 gttgccggta atcaaatatg catccagcga tagcgccccg agaatatgct gcatctccag
    55801 cgcatgacgc cccgtggcaa aagtgagggt aatgtcgcgt tcacgcagtc gcgccaaagt
    55861 agagagggtt ttctcaccta aatgatggtc aggcatcaat aaagtgccat ccatatcaaa
    55921 tgctgccaga cgagccattt ctttctccac tctgtgacac ggttaatggt gattgagtat
    55981 cacctgatat atacggaagt aatagtgaat agttaaataa tattgttccg ggtttatatg
    56041 cgattgctca accgtcttaa ccagtatcaa cgtctgtggc aaccttcagc cggaaagccg
    56101 caaaccgtca ccgtcagcga actggccgaa cgctgttttt gcagcgaacg ccatgttcgt
    56161 acgctgttgc gtcaggcaca ggaggcggga tggctggagt ggcaggcgca gtcaggacgc
    56221 ggaaagcgcg gacaattacg ctttctggtc acgccggaat cgctacgcaa tgcgatgatg
    56281 gaacaggcac tggaaaccgg aaagcagcaa gatgtgctgg agctggcgca actggcccca
    56341 ggtgagctgc gcactctgtt acagccgttt atgggcggac aatggcaaaa cgatacaccc
    56401 acgttgcgta ttccctacta tcgcccgctc gaaccgctac aaccaggctt tttgcccggc
    56461 cgtgccgagc agcatctcgc cgggcagata ttttccggcc tgacccgctt cgataataat
    56521 actcagcgcc cgattggcga tttagcgcat cactgggaaa cctctactga cgggttacgc
    56581 tgggactttt atcttcgttc aaccctacac tggcataacg gcgatgcagt aaaagcctca
    56641 cacttacacc agcgattatt gatgctgtta caactgccag cactggatca attatttatt
    56701 agcgtgaagc gtattgaagt cacccatccg cagtgtctga ccttcttttt acatcgcccc
    56761 gattactggc ttgcgcaccg gctggcaagc tattgcagcc atctggcgca tccgcaattc
    56821 ccactgatcg gcactggtcc ttttcgctta acacaattca cagcggagct ggtgcgcctg
    56881 gagagccatg attattacca tttgcgtcat ccgctgctta aagcggttga gtactggata
    56941 actccgccgc ttttcgaaaa agatttggga accagttgtc ggcatcccgt gcaaatcacc
    57001 atcggcaaac cggaggagct gcaacgggtc agccaggtca gtagtggcat cagtttaggt
    57061 ttttgctatt taacgttgcg caaaagtccc cgactctccc tctggcaggc gcgaaaagtg
    57121 atctccatta ttcatcaatc cggtttatta caaacgttag aagtcggaga aaacctgatc
    57181 accgccagtc atgcattact gccaggctgg actattccgc actggcaagt accggatgaa
    57241 gtcaaactac cgaaaacctt gacgctggtt tatcacctac cgatagaact tcataccatg
    57301 gcagaacgcc tacaggcgac actggcagcg gaaggctgtg aactcacaat tatttttcat
    57361 aacgcaaaaa actgggacga cacgacccta ctggcacacg cagacctcat gatgggcgac
    57421 agattaattg gcgaagcacc ggaatatact ctggagcaat ggctacgttg cgatccacta
    57481 tggccacatg ttttcgacgc tccagcatat gcacatctgc aatcgacgct ggacgcggtg
    57541 caagtaatgc ctgatgagga aaaccgattt aatgccctga aagcggtttt tagccagtta
    57601 atggcagacg cgacgctgac gccgctgttc aactatcact atcgcattag tgcccctccc
    57661 ggcgtgaacg gtgtgcgact gacaccgcgc ggctggtttg aatttaccga agcctggctt
    57721 cccgcgccgt cgcaatgaat agctggtccg ggttaatcgc aggcgttacc ataacgcttt
    57781 tattgttcaa gcaggattat ctatgaaacg tgctgtcgtt gtgttcagtg gaggccagga
    57841 ttccaccacc tgtctggtgc aggcattaca acaatatgat gaagtccatt gcgtgacgtt
    57901 cgattacggt cagcgacatc gcgcagaaat cgacgtggca cgcgaactgg cgctgaaact
    57961 gggagcacgc gcgcataagg ttcttgatgt aacgctgctc aacgagctgg cggtcagcag
    58021 cctgacacgt gacagcattc cggtgcctga ttatgaacct gaagccgatg gcatcccgaa
    58081 tacgtttgtc cctgggcgta atattttgtt cctgacgctg gcggcaatat atgcgtatca
    58141 ggtaaaagca gaagccgtaa ttaccggcgt ctgtgaaacg gatttctccg gctacccgga
    58201 ttgccgcgat gagtttgtga aagcactaaa ccatgccgtc agtttgggca tggcgaaaga
    58261 tattcgtttt gaaacgccgc tgatgtggat tgataaagcg gaaacctggg cgctggcaga
    58321 ttattacggc aaactggatt tagtccgtaa cgaaacgttg acctgctata acggcattaa
    58381 aggcgacggt tgcggtcatt gtgcggcatg taatttacgc gccaacggtt tgaatcatta
    58441 tctggccgat aaaccgacgg tgatggcagc gatgaagcag aaaaccgggt tgaggtaatt
    58501 attccgggtg tcgccggatg cggcttgagc atccggcacc acaaaacgtt tacttaacca
    58561 tctgctccag cttttcgcgc aattcccctt ccagagctaa tgctttctgc gttttaagat
    58621 caatacaaac aaacgtaata agcgcatccg ctaccacctg cccttccggc tccagtgtaa
    58681 tgacctggct taagatgccg cttttaccgt ttaattgctg caactgactg gtaatagtta
    58741 acaggtcact taataccgct gggcgacgat agttaatatt gatattgacc acgacgaagg
    58801 cgatgttatg ggccgtcatc cactgaaaac tgtcgctatt ttccaaccca tcccagcggg
    58861 cttcttcgag aaattcaagg tagcgggcgt tgttgacgtg ctggtaaacg tcgagatgat
    58921 atccacgaac tttgatttgt gtttgcatag cgcaataacc ttacgttgtt gttataagta
    58981 cagaggtcat aactggtatg acctcttcag tctggcaaaa aattgccact atgcaaatta
    59041 attacagggt taataccgcc agattacgtt ccaccagcga attgcccatc cccggcacct
    59101 gctttagatc ctccaccgtt ttaaacggac cgtactcttc gcgataactg acaatcgcct
    59161 gcgctttctt caggccaacg ccattcatcg cgcgggctag ctcttccgcg ctggcattat
    59221 taatgctgac ccgggtgcct tcttcgtcac tggctttcgc cggtactgct gctttacttt
    59281 gtgctgcagg agcttccgct ttggtttcta ccgttgtcgg tttcgccaca gaagctgccg
    59341 ccagcgcgct atgaggacat tccggcacag gccagggaca gggtaatgag cagtgcttta
    59401 attccgtgtt tcatactgtt ttctccttgt ttgttaacag tggaatcacc ttaacggcag
    59461 tgaaaacagt aaacaaatgg caatgttcag aaatggaaaa ggccgcgaaa gcgacctttt
    59521 cttgttacag ctgcaacgca tttttttgcg aggcggcttc aggattattg ctgttccagc
    59581 gcatcgccaa ttttgatttt cgcctcttta cgcaggttac tcatcagagc ttcaaagacg
    59641 atttgtgcgt tgttctgggt gataccctgc accatcgctt ttttctgatc ttccggcatt
    59701 gaaccttgtt tcacttcatc cagcgccagc agaaccacat taccttgcat atcggtcgcc
    59761 ataccgtagc tcggtttgtc tttcgctggc agtggcagtg caaacgccgc ctggctaatc
    59821 gggtcacgac cggaacggct taagattttc ggctcgccaa atttcagacc ggcagcctgc
    59881 atagcttccg cacctttgcc ggctttcaaa tcaaccagca gtttctcagc atccactttc
    59941 gcctgttgtt cagctttgtt gtgctgaacc agtgccttaa cttgttcctg aacatctgcc
    60001 aacggtttca ccgcttccgg tttgtgctcg ctgatgcgca gcacgaatgc gcggtcgccg
    60061 tctacggtga tgatgtcaga gttgatgccc ggcgcgccgt tttcacctac cagaccgccg
    60121 ttaaagatag cgtcggcaac cggcttgaag ttcaactctt ccggcaggtt atctttgcta
    60181 aaccaacccg tctgagtggc tttaacgccg gcagcttgct ctgcaccggc cagagactcg
    60241 gtgtcgttgc ttgccgcatc gctcactttc tgctgcagcg catagtacgc atcgagggct
    60301 ttttcgtgtt tcactttcgc cgcaatgtcg tcacgtactt cgtctaacga tttcactttc
    60361 gctggctgaa tgtcgtccag acgtacaatc aggaaaccga ccgaagattt gatgacacca
    60421 gacagttgcc ctttttcttt cagaccagca tttttcagtt cgtcggggat agtggcatct
    60481 tctaaccaac ccatatcgcc gccgttacgg gcagagataa tatcggcaga tttttctttg
    60541 gctaatgcag caaaatcacc gccttcattc agctcatcaa gtaccgcttt cgcttcatct
    60601 tcagttttgg tctggatgat gctgtagcgg gtacgctgcg gctgggtgaa ttgatcctga
    60661 tgctgatcgt agtagctctg gatatccgca tcgctaaccg gttgctgcat cgttgcggca
    60721 tccagcttga tgtaactcac gcggaattgt tccggcgtca tgaaattgtt tttgttttgt
    60781 tcgtagtagc tggcaatctc ctgttcggtc acaggctgct tcgccgccag cgcgttaaca
    60841 tcgatagtcg cctcacgcac cacgcgttgt tgcgcgacca gtgccgccag ctcgtcggtt
    60901 tcacctttca gcataaaatc ggtaccggca acaccgttaa tcagctgttg ggtagtgagc
    60961 tggttacgca gcgcctgggc gtactgatcg gcggtcatcc ccatctggtt gaggataccg
    61021 ttatagcggc tgttatcaaa tttgccgtca acctggaagg ctggggtcgc gaaaatcgcc
    61081 tgtttaacct gctcatcact gatacccagt ttcagctcac gagcgtactg atccaacagc
    61141 gcctcgtcga tcagacgatt cagcacctgt tgacgcaggg ttttcatata gccttcgttc
    61201 gctgccagct cggagtattg atcgcccagc tgttgctgca tgcgattacg ctcgctgtta
    61261 aaggcgttct cgaactgccc acggctgatt tcctggtcat tcacttttgc ggcgtagtta
    61321 ttgcctccgc caatcaggta accactcacg ccggtcaata tgaacgacac gataatgata
    61381 ccgaaaataa tcttgagcac gagactgttt gcagccgtgc gtaagctgtc catcatggtg
    61441 taacaacact ccgctgtaga tgactgtgta cctcacgcaa cgtatcgatg cgcgcaaagg
    61501 cctattgtga caagaaacgg gggcaattgt cagcccacaa gacgcagaaa gtcaccgaat
    61561 acaaataaaa aaggcacatc agtagatgcg cccttgaact tcgtcacatc cccaccgggg
    61621 acaacgctta gtttaccgcg tctttcagtg ctttacctgc acggaagctc ggtactttag
    61681 cagcagcgat ggtgatctct ttaccggtct gcgggttgcg gccagtacgg gcagcacgct
    61741 ctttaacggc aaaagtacca aaacctacca gtgctacatc atccccttct ttcagagatt
    61801 cagttacgga agcaataata gcatctaacg cacggccagc cgcagcttta gagatatcag
    61861 cccctgcagc aatcttgtcg atcaattgag atttattcac tcttctcttc ctctttataa
    61921 tttatatcgc acttgaatcc ttcaaggtac gaacgcgcag cagttatatc aggccagcca
    61981 tccccttaca acacccctta ataagggcaa gcccgatccg ccatctaact tagcgagaca
    62041 aaaaaaggct ggcaagcccg aattagcctg ccagccctgt ttttattagt gcattttgcg
    62101 cgaggtcact attttgcagt cacaacctgc ataccagacg gttcattttg tagcgccaga
    62161 gtcagaactt cctcaatgcg cttcacagga tgaatgtcca gatcggcaat tacgttgtca
    62221 ggaatctctt ccagatcgcg tttattttcg aacggaatta gcactgtttt aatcccgccg
    62281 cgatgcgctg ccaggagttt ttctttcaaa ccaccgatcg gcagtacctg accacgcaga
    62341 gtgatctcac cggtcattgc cacatcggca cgaaccgggt taccggtcag gcaagaaacc
    62401 agcgcggtgc acatagcaat accggcactc ggaccatctt tcggcgtcgc accttccgga
    62461 acgtggacgt ggatgtcacg tttttcgtaa aaatcagggt tgatccccag tttttccgca
    62521 cgcgcacgaa ccaccgttaa cgccgcctga atagactcct gcatcacttc gccgagcgaa
    62581 ccggtatagg tcagtttgcc tttacccgga acacatgcgg tttcaatggt cagcaagtca
    62641 ccgcccactt ccgtccacgc cagaccggtt acctgaccga cacggttttc gttatcagcg
    62701 cgaccatagt cgaaacgctg aacaccgagg tagtcatgca ggttatcgcc gttaatttcg
    62761 atatgtttta atgacttatc gagcagtaac tgcttaaccg ctttgcggca cagtttggag
    62821 atttcacgct ccagaccacg cacgcccgcc tcacgggtgt agtaacgaat aatgccgata
    62881 atggcgctat cgtcgacggt cagctcacct tttttcagtg cattacgttc aatctgcttc
    62941 ggcagcaggt gacgtttggc gatgttcagt ttttcatctt cggtataacc ggagaggcga
    63001 atcacttcca tacgatccag cagcggtgcc ggaatgttca tggagttcga cgtcgcgaca
    63061 aacatcacgt cgctgagatc gtaatccact tccaggtagt ggtcgctgaa cgctacgttc
    63121 tgctctggat ccagcacttc aagcagtgca gaggccggat cgcctcgcat gtcagaagac
    63181 attttgtcga tctcatcgag caggaacagc gggtttttca cgcccacttt cgccattttc
    63241 tggatcagtt tacccggcat agaaccgatg taagtacggc ggtgaccacg gatttccgct
    63301 tcatcacgca cgccgcccag cgccatacgg acatatttac gcccggtggc tttggcaatg
    63361 gactgaccaa gagaggtttt acctaccccc ggcggcccta ccaggcagag gatcggtccc
    63421 ttgattttgt tgacacggct ttgaaccgca aggtactcaa ggatgcgatc tttcacgcgc
    63481 tccagaccat aatggtcggt atcaaggatt tcctgcgcct gacgcaggtc ttttttgacc
    63541 ttgctgcgcg cattccacgg tacctgtacc atccagtcga tataaccacg cactacggtc
    63601 gcttctgccg acatcggaga catcattttc agcttctgca actctgcttc cgctttctct
    63661 tttgcctctt tcggcatctt cgccgcgtcg attttgcgct tcagggcttc gttttcgtcc
    63721 ggcgcgtcgt ccatttcacc gagttctttc tgaatagctt tcatttgctc gttcagatag
    63781 tactcacgct gggatttctc catctgcttt ttaacgcggt tgcgaatgcg tttctcaacc
    63841 tgcagcagat cgatttccga ttccatcatt gccatcagat attccagacg ttcgttaacg
    63901 tcggacatct ccagaacgga ctgtttgtca gccagtttca gcggcatatg tgcagcaatg
    63961 gtatccgcca gacgcgccgg atcgtcgatg ctattcagcg acgtcagcac ttctggtggg
    64021 atttttttgt tcagcttgat gtagccttcg aactggctga ttgcagtacg caccagcact
    64081 tcctgttccc gctcatcaat ggtcggcgac tccagatact ccgccttcgc agaaaagtgt
    64141 tcgccattgt cagagagcgc agaaatacgc gcgcgctgta acccctcgac cagcactttg
    64201 acggtgccgt caggcagttt cagcatctgc aatatagagg ccacggtccc gacggtgaaa
    64261 agatcgttta cacccggctc atccgttgaa gcttctttct gcgcgaccag cataattttt
    64321 ttatcatggt ccatcgccgc ttccagacaa cggatagatt tttcccgccc gacaaataag
    64381 gggatgacca tgtgcggata aaccaccaca tcgcgcagcg gcaatacggg gatttcaatg
    64441 cgttcagaac gctcaggatt catagagctc tctcttagtt taatttccgc caggtaatca
    64501 gatgacacga ctgtgcttca cgccatctat taacatgtac gtcagtatat ggggatgttt
    64561 cccccacatt caacgccgag aatagaggaa aaattaaagg ggagataaaa tccccccttt
    64621 ttggttaact aattgtatgg gaatggttaa ttattcacca gatgcctgtt gcgcttccgg
    64681 cttgccataa atcagcaacg gtttgctttg accatcaatt accgactcgt cgataaccac
    64741 tttttcgaca tcttccatgg acggcagatc gtacatggta tcgagcagtg cggcttctac
    64801 gatggaacgc aggccacggg caccggtttt acgcgccatc gctttcttag cgatagcatc
    64861 cagcgcctcg tcacggaatt ccagatccac gccttccaga ttaaacagcg cctgatactg
    64921 cttggtcagg gcgtttttcg gctctttgag gatctgaatc agagcttctt cgctcagttc
    64981 attcaacgtt gcgacaaccg gcagacgacc aatgaactca gggatcagac caaacttgat
    65041 cagatcttcc ggttcaacct gcgccagcag ctcgccttcg cttgctttgt cggacttcgc
    65101 ttttaccgtc gcgccaaaac caatgccgga gccggtttct acacgatggg aaatcacttt
    65161 atccagaccg gcaaacgcac cgccacaaat aaacaggatc ttagaggtat caacctgcaa
    65221 gaattcctgc tgcggatgtt tacgcccacc ttgcggtgga acagcagcta ccgtaccttc
    65281 gatcagtttc aacagtgcct gctgtacgcc ttcaccggaa acgtcacggg taatagacgg
    65341 gttgtctgac ttacgagaaa tcttgtcgat ttcatcgatg tagacaatcc cgcgctgcgc
    65401 tttctgtacg tcgtaatcgc acttttgcaa cagcttctga atgatgtttt caacgtcttc
    65461 gcccacataa ccggcttcgg tcagcgtggt tgcgtcggcc atggtgaacg ggacgtccag
    65521 caggcgcgcc agcgtttcag ccagcagcgt tttaccggaa ccggtcggac cgatcagcag
    65581 aatgttactt ttgcccaact cgacaccatt gctggtatcg ccgttgcgca gacgtttgta
    65641 atggttgtat accgcgaccg ccagcacttt tttcgcctgt tcctggccga taacgtaatc
    65701 gtccaggtgg ttgcgaattt catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc
    65761 aacttcttta atctcttcgc gaatgatgtc gttacataaa tcaacacatt cgtcgcagat
    65821 atacacggat ggaccggcaa tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga
    65881 gcaatacagc aatttgcctg agccatcttt gcgtttatct gtcatgggtc aaaacctctt
    65941 ctttgttctt tgtgccgcac acgacgacgc aaatgccatt ctcaggcgca agccgcttac
    66001 agcgttgtgc cgccctggat aagtatagcg gcacagttgc gcctctggca tcaattacga
    66061 tgggtcagaa tcgaatcgac cagaccgtat tccaccgctt caggggcgga aaggaagcga
    66121 tcgcgctcgg tatcacgttc aatctgttct aatgattgac ccgtatgaag cgccataagt
    66181 tcattcatgc gccctttaac tttcagaatt tcacgggcat gaatttcgat atcggtcgcc
    66241 tggccctggt agccgcccaa cggttggtga atcatcacgc gcgaattcgg caggcaaaaa
    66301 cgtttacctt ttgcccctgc ggtcagcaag aaagcgccca tcgaggccgc ctggcccata
    66361 cagatggtgc tgacatcagg cttgataaac tgcatggtgt catagataga catcccggca
    66421 gtgatcaccc cgcctgggga gttaatgtac agatagatat ctttttctgg gttttccgct
    66481 tccaggaaca gcatctgcgc cacaatcagg ttagccatgt ggtcttcaac ctggccagtc
    66541 agaaaaatga cgcgttcctt aagtagacga gaatagatat caaaagagcg ctcaccgcgt
    66601 gaggtctgtt caatgaccat cggcaccagc gccatatggg gtgcaaagtt atctcgttcg
    66661 ccgctgtatg acatttccgt ctcctggata aaattgaaaa aacctgctgt accgattgta
    66721 accttatgga cggattatca gctagtccct gtgttatggg tacggcatca ccatatttca
    66781 agcataacaa tcttttgttg ttacgctaac accgaaagag gcttttcgca cggttccatg
    66841 caaaattcat gacaaaaaaa agcccaccac ctggcggtga cgggcctttg tgcgaattta
    66901 gcgcgttatg ctgcgtaaat tacgcctgct ggttcatcag ctcgttgaaa gtggtttctt
    66961 tttcagtcac tttcgctttc gccagtacag cttcaacagc ctgttcttcc agagcaacat
    67021 tgcgcatgtt gtccatcagt tctttgtttt tgctgtagaa ctcgataact tctttcggat
    67081 cttcgtacgc agaagccatc tcttcgatca ggcctttcac gcgctcttcg tcagctttca
    67141 gctcgttggt gcggataact tcgcccagca gcaggccaac aactacgcga cgtttagcct
    67201 gttcttcgaa cagttcgcgc ggcagttcca gagcttgttt ttcgttgcca ccgaaacgct
    67261 gtgcagcctg gcgacgcaga acgtcgattt cgctgtcgat cagcgcagcc ggtacgtcga
    67321 tgtcgttagc ttttaccaga ccttcgatcg cctgagactt aacgcggtta cggatggcgc
    67381 tcttcagctc gcgctccatg tttttacgca cttcagcgcg cagaccttct acggaaccat
    67441 cttcaacgcc gaaacgtttg atgaattctg cagtcagttc cggcagttca cgctcttcaa
    67501 ctttcttcag gttgatagcg aatttcgctg ctttaccttt caggttttct gcgtggtatt
    67561 cttccgggaa ggtcacgtcg atggtgaact cttcgccagc tttgtggcct ttgataccgt
    67621 cttcaaagcc cgggatcata cgaccctggc ccatcgccag tacgaaatca gacgctttac
    67681 cgccttcgaa ctcttcgccg tctacagaac cggtgaagtc gatggttacg cggtcttctg
    67741 cttcaacagc gccgtctttt tctttccagg tcgcctgctg tttacgcaga gtatccagca
    67801 tgccgtcaac gtcagcgtcg gtcacttcaa cgatcggttt ttcaacttcg atcgcttcca
    67861 gaccctgcag ttcaacttcc ggataaactt caaactctac agagtaagtg aagtcttcac
    67921 ccagcttgta ttcgcccgga acataagtcg gtgcgccagc cggattgatt ttttctttaa
    67981 tgatggcgtc aatgaagtta cggctcatca ggtcacccag aacgtcctgg cgtacagacg
    68041 cgccataacg ctgagcaacg atattcattg gcactttgcc tttgcggaag ccgtcaatac
    68101 gtactttttt cgcaacgttg accagctcgc ttttaacagc ggtctcgatg ctgtcagcag
    68161 cgatagtaat cgttacacgg cggccaaggc cttgagtggt ttcaactgaa acttgcatct
    68221 tgttacctca aaaaatcaca gtgctcggtc aactcttacc ccgcaagcac aggttgttgg
    68281 cttcgcggaa ccgggatgtt cttataatca atcacattcc ctgttaccag aatcatcccg
    68341 aaagcattca aataggacgc ggcattatag cggcatcact tttatgagtc gagaacggtt
    68401 attgcgcgct gtcgcgtatt ttttcacaat tcccgggcaa ttttcgtctg aatgctgggc
    68461 aaaaagcgca aaattcagac aaaacaaaac ggcccgtagg ccgtttttca taaaatcttt
    68521 agcaacatac tggaaaagcg ccgacagttg caaatgcgtt tttacgcgat gcttcctgct
    68581 ccacggcagg gaggagaggc aaagacggtg tcctgcaacc cttcccactc cttaatagtg
    68641 taagtatgca gagccagcgc atgaacggta gtagagagtt cctccgctaa agtactgtaa
    68701 atcattcgat gacgattcag aaaacgttca cccgtaaaac gatcgctgac cagcacaact
    68761 ttaaaatggc tttcagagcc ggctgggaca ttgtgacgat agctttcatc cactacttcg
    68821 aggaatacgg gttggaacgc cgcccttaat ttttcttcta tccgctcacg tatcatcatg
    68881 aaatatctcc tccgacaacg ctgagatgtc atccatccct ttaaatacta gccgctttta
    68941 ccgtttccat tgcagcttaa caacaaatat ttaggtttcg tcctggtttt tccgtcaagc
    69001 gtatgaagtt tactgattta aagattgaaa cacaggcctc gcatcagtgt ttcttttgcc
    69061 aggggcgatt atttcgtcaa catgatgaat ttacatgcct tacccacttc ccctcgccgt
    69121 tggcaatgtt atgatggcgg gaatttttac caaacacgct taagagtctc tgagagcccg
    69181 aacatgttca aaaaaatcct cttcccgtta gttgctctgt ttatgcttgc aggatgcgca
    69241 aaaccgccaa caactattga agtttccccg acgattacac tgccacagca ggatccaagc
    69301 ctgatgggcg tcaccgtaag cattaatggt gccgatcagc gtaccgatca ggcgctggca
    69361 aaagtcaccc gcgataatca aatcgttacc ctgaccgcct cccgcgatct gcgtttcctg
    69421 ctgcaagaag tgctggaaaa acagatgacc gcgcgtggtt acatggttgg tccgaatggc
    69481 ccggttaatc tgcaaatcat cgttagccaa ctgtatgctg acgtgtccca gggcaacgtg
    69541 cgctataaca tcgctaccaa agcggatatc gccatcatcg ctaccgcgca gaatggcaac
    69601 aaaatgacca aaaactatcg tgccagctac aacgttgaag gtgcgttcca ggcctccaac
    69661 aaaaatatcg ccgatgcggt taacagtgtg ctgaccgata ccatcgctga tatgtctcag
    69721 gacaccagca ttcacgaatt tatcaagcag aacgcgcgtt aatttctgcc ctctggcccg
    69781 gtgcaaaccg ggcctgtaga cgcccatgtc cagtcaatat ttacgtattt ttcaacagcc
    69841 gcgttcagcc atattgctga tcctgggttt tgcttccggg ctaccgctcg ccctgacatc
    69901 cggtacctta caggcctgga tgacggtcga gaatattgat ctcaaaacca ttggtttctt
    69961 ctctctggta ggccaggctt acgtttttaa attcctctgg tcaccgctga tggaccgcta
    70021 cacgccccca ttttttgggc ggcggcgcgg ttggctgctc gccacgcaaa tcctgttatt
    70081 agtcgccatt gcggcgatgg gttttctcga acctggcacc caactccgct ggatggcggc
    70141 actggcggtg gtgatcgctt tttgctcggc ctcccaggat attgtcttcg atgcgtggaa
    70201 aaccgatgtg ctcccggcag aagaacgtgg tgcgggcgcg gcaatcagcg tgctgggtta
    70261 ccgtttaggg atgctgattt ccggcggctt agccctgtgg ctggcagata aatggctggg
    70321 ctggcagggc atgtactggt tgatggcggc gctgttgatc ccttgtatta tcgcgacgtt
    70381 gcttgcacca gaaccgaccg acaccattcc tgtgccaaaa acgctggaac aagcggttgt
    70441 tgcacctctg cgagatttct ttggtcgcaa taatgcctgg cttattttgc ttcttatcgt
    70501 gctgtataag ctgggcgacg cattcgccat gagcctgaca accacgtttt tgattcgcgg
    70561 cgtcgggttt gatgcgggtg aagtaggcgt ggttaacaaa acgcttggct tactggcgac
    70621 cattgtgggc gcattgtacg gtgggatttt gatgcagcgc ctgtcgctgt tccgggcgct
    70681 gctgattttc ggcattttac aaggtgcgtc taacgctggt tactggctgc tgtcgattac
    70741 tgataagcat ctctacagca tgggcgcagc cgtctttttc gaaaacctct gtggcgggat
    70801 gggcacatca gcctttgtcg cgctgttaat gacgctgtgt aataagtcat tttccgcaac
    70861 tcaatttgcc ctgctctcag cgctttccgc tgtaggacga gtttatgtcg gccccgtggc
    70921 gggttggttt gttgaagcac acggctggtc gacattctat ctattctccg tcgccgctgc
    70981 cgtaccgggg cttattttgc tgctggtttg ccgccagacg cttgaatata cacgagtaaa
    71041 tgacaacttt atctcccgta ccgaatatcc ggcaggttat gcctttgcca tgtggacact
    71101 ggcggcgggc gtcagcctgt tggccgtgtg gttactgctg ttgacgatgg acgcgctgga
    71161 tttgacgcac ttctctttcc tgcctgctct gctggaagtc ggggttttag tcgccctttc
    71221 tggcgtcgtg cttggtggtt tgctggatta tctggcgcta cgaaaaacgc atctgacgta
    71281 atctgtaaat attatttagc tgtagtaatc actcgccgta attattacgg ctaaataaac
    71341 atcagtatcg cttaataata tttgttcttt tgtgcggctt agcgtttgga aattattgac
    71401 gcaatttatg aattctattt tttcttacac gattcagcta atgagtcttt attttccccc
    71461 cacccaattg tcaccataat gataattatt tgttaaacaa ttgttttatt ccagattggt
    71521 tataccaatt gcccgtccaa actccttctg cactcccctt tcgttataac gcccttttgc
    71581 aacagcttct taaaatcaac ttgatatatt ttgcaacata tgtgacctga cagccaaatc
    71641 caagtaacag gaatttaatc atgtttacag taatgtaacc ttcccgtaaa atgcccacac
    71701 actttaaacg ccaccagatc ccgtggaatt gaggtcgtta aatgagactc aggaaataca
    71761 ataaaagttt gggatggttg tcattatttg caggcactgt attgctcagt ggctgtaatt
    71821 ctgcgctgtt agatcccaaa ggacagattg gtctggagca acgttcactg atactgacgg
    71881 catttggcct gatgttgatt gtcgttattc ccgcaatctt gatggctgtt ggtttcgcct
    71941 ggaagtatcg tgcgagcaat aaagatgcta agtacagccc gaactggtca cactccaata
    72001 aagtggaagc tgtggtctgg acggtaccta tcttaatcat catcttcctt gcggtactga
    72061 cctggaaaac cactcacgct cttgagccta gcaagccgct ggcacacgac gagaagccca
    72121 ttaccatcga agtggtttcc atggactgga aatggttctt catctacccg gaacagggca
    72181 ttgctaccgt gaatgaaatc gctttcccgg cgaacactcc ggtgtacttc aaagtgacct
    72241 ccaactccgt gatgaactcc ttcttcattc cgcgtctggg tagccagatt tatgccatgg
    72301 ccggtatgca gactcgcctg catctgatcg ccaacgaacc cggtacttat gacggtatct
    72361 ccgccagcta cagcgggccg ggcttctcag gcatgaagtt caaagctatt gcaacaccgg
    72421 atcgcgccga attcgaccaa tgggtcgcaa aagcgaaaca gtcgccgaac accatgtctg
    72481 acatggctgc gttcgaaaaa ctggccgcgc ctagcgaata caaccaggtg gaatatttct
    72541 ccaacgtgaa accagacttg tttgccgatg tgattaacaa gtttatggct cacggtaaga
    72601 gcatggacat gacccagcca gaaggtgagc acagtgcaca cgaaggtatg gaaggcatgg
    72661 acatgagcca cgcggaatcc gcccattaaa ggggttgagg aagaataaag atgttcggaa
    72721 aattatcact tgatgcagtc ccgttccatg aacctatcgt catggttacg atcgctggca
    72781 ttattttggg aggtctggcg ctcgttggcc tgatcactta cttcggtaag tggacctacc
    72841 tgtggaaaga gtggctgacc tccgtcgacc ataaacgcct cggtatcatg tatatcatcg
    72901 tggcgattgt gatgttgctg cgtggttttg ctgacgccat tatgatgcgt agccagcagg
    72961 ctcttgcctc ggcgggcgaa gcgggcttcc tgccacctca ccactacgat cagatcttca
    73021 ccgcgcacgg cgtgattatg atcttcttcg tggcgatgcc tttcgttatc ggtctgatga
    73081 acctggtggt tccgctgcag atcggcgcgc gtgacgttgc gttcccgttc ctcaacaact
    73141 taagcttctg gtttactgtt gttggtgtga ttctggttaa cgtttctctc ggcgtgggcg
    73201 aatttgcgca gaccggctgg ctggcctatc caccgctatc gggaatagag tacagtccgg
    73261 gagtcggtgt cgattactgg atatggagtc tccagctatc cggtataggt acgacgctta
    73321 ccggtatcaa cttcttcgtt accattctga agatgcgcgc accgggcatg accatgttca
    73381 agatgccagt atttacctgg gcatcactgt gcgcgaacgt actgattatt gcttccttcc
    73441 caattctgac ggttaccgtc gcgttgttga ccctggatcg ctatctgggc acccatttct
    73501 ttaccaacga tatgggtggc aacatgatga tgtacatcaa cctgatttgg gcctggggcc
    73561 acccggaagt ttacatcctg atcctgcctg ttttcggtgt gttctccgaa attgcggcaa
    73621 ccttctcgcg taaacgtctg tttggttata cctcgctggt atgggcaacc gtctgtatca
    73681 ccgtgctgtc gttcatcgtt tggctgcacc acttctttac gatgggtgcg ggcgcgaacg
    73741 taaacgcctt ctttggtatc accacaatga ttatcgccat cccgaccggg gtgaagatct
    73801 tcaactggct gttcaccatg tatcagggcc gcatcgtgtt ccattctgcg atgctgtgga
    73861 ccatcggttt tatcgtcact ttctcggtgg gcgggatgac tggcgtgctg ctggcagtac
    73921 cgggcgcgga cttcgttctg cataacagcc tgttcctgat tgcgcacttc cataacgtga
    73981 tcatcggcgg cgtggtcttc ggctgcttcg cagggatgac ctactggtgg cctaaagcct
    74041 tcggtttcaa actgaatgaa acctggggta aacgcgcgtt ctggttctgg atcatcggct
    74101 tcttcgttgc ctttatgccg ctgtatgcgc tgggcttcat gggcatgacc cgtcgtttga
    74161 gccagcagat tgacccgcag ttccacacca tgctgatgat tgcagctagc ggtgcagtac
    74221 tgattgcgct gggtattctc tgcctcgtta ttcagatgta cgtttctatt cgcgaccgcg
    74281 accagaaccg tgacctgact ggcgacccgt ggggtggccg tacgctggag tgggcaacct
    74341 cttccccgcc tccgttctat aactttgccg ttgtgccgca cgttcacgag cgtgatgcat
    74401 tctgggaaat gaaagagaaa ggcgaagcgt ataaaaagcc tgaccactat gaagaaattc
    74461 atatgccgaa aaacagcggt gcaggtatcg tcattgcagc tttctccacc atcttcggtt
    74521 tcgccatgat ctggcatatc tggtggctgg cgattgttgg cttcgcaggc atgatcatca
    74581 cctggatcgt gaaaagcttc gacgaggacg tggattacta cgtgccggtg gcagaaatcg
    74641 aaaaactgga aaaccagcat ttcgatgaga ttactaaggc agggctgaaa aatggcaact
    74701 gatactttga cgcacgcgac tgcccacgcg cacgaacacg ggcaccacga tgcaggcgga
    74761 accaaaatct tcggattttg gatctacctg atgagcgact gcattctgtt ctcaatcttg
    74821 tttgctacct atgccgttct ggtgaacggc accgcaggcg gcccgacagg taaggacatt
    74881 ttcgaactgc cgttcgttct ggttgaaact ttcttgctgt tgttcagctc catcacctac
    74941 ggcatggcgg ctatcgccat gtacaaaaac aacaaaagcc aggttatctc ctggctggcg
    75001 ttgacctggt tgtttggtgc cggatttatc gggatggaaa tctacgaatt ccatcacctg
    75061 attgttaacg gcatgggtcc ggatcgcagc ggcttcctgt cagcgttctt tgcgctggtc
    75121 ggcacgcacg gtctgcacgt cacttccggt cttatctgga tggcggtgct gatggtacaa
    75181 atcgctcgtc gcggcctgac cagcactaac cgtacccgca tcatgtgcct gagcctgttc
    75241 tggcacttcc tagatgtggt ttggatctgt gtgttcactg ttgtttatct gatgggggcg
    75301 atgtaatgag tcattctacc gatcacagcg gcgcgtccca tggcagcgta aaaacctaca
    75361 tgacaggctt tatcctgtcg atcattctga cggtgattcc gttctggatg gtgatgacag
    75421 gggctgcctc tccggccgta attctgggaa caatcctggc aatggcagtg gtacaggttc
    75481 tggtgcatct ggtgtgcttc ctgcacatga ataccaaatc agatgaaggc tggaacatga
    75541 cggcgtttgt cttcaccgtg ctaatcatcg ctatcctggt tgtaggctcc atctggatta
    75601 tgtggaacct caactacaac atgatgatgc actaagagcg gcggttatga tgtttaagca
    75661 atacctgcaa gtaacgaaac caggcatcat ctttggcaac ctgatctcgg tgattggggg
    75721 attcctgctg gcctcaaagg gcagcattga ttatcccctg tttatctaca cgctggttgg
    75781 ggtgtcactg gttgtggcgt cgggttgtgt gtttaacaac tacatcgaca gggatatcga
    75841 cagaaagatg gaaaggacga agaatcgggt gctggttaaa ggcctgatct ctcctgctgt
    75901 ctcgctggtg tacgccacct tgctgggtat tgctggcttt atgctgctgt ggtttggcgc
    75961 gaatccgctg gcctgctggc tgggggtgat gggctttgtg gtttatgtcg gcgtctatag
    76021 cctgtacatg aaacgccact cagtctacgg cacattgatt ggttcgctct ccggcgctgc
    76081 gccgccggtg attggctact gtgcagtaac cggcgagttc gatagcggcg cagcgatcct
    76141 gctggctatc ttcagcctgt ggcagatgcc tcactcctat gccatcgcca ttttccgctt
    76201 taaggattac caggcggcaa acattccggt attgccagtg gtaaaaggca tttcggtggc
    76261 gaagaatcac atcacgctgt atatcatcgc ctttgccgtt gccacgctga tgctctctct
    76321 tggcggttac gctggatata aatatctggt ggtcgccgcg gcggttagcg tctggtggtt
    76381 aggtatggct ctgcgcggtt ataaagttgc tgatgacaga atctgggcgc gcaagctgtt
    76441 cggcttctct atcatcgcca tcactgccct ctcggtgatg atgtccgttg attttatggt
    76501 accggactcg catacgctgc tggctgctgt gtggtaacaa aacctctcta ttaaaaaggt
    76561 gctacggcac cttttttatt gtctgagatc agagatacgg ctaataaccg tatgtcttga
    76621 cagagtgagc agatgaacat aaattaatca tacggttgat agccgtatta tgtatgagat
    76681 cgactcccta cgatttcatt cagttaatca aagaggtcat aatgcctgga aaaacagcca
    76741 ctctacccga tgtcgataaa acgctgaaaa atgcgcgggt cgaactcaaa actagtcccg
    76801 acgccaaaaa caaacttcgt gaagctgcgc aagccgttgg ggtagattta agcgctttta
    76861 ttttgagcgc tgctatggaa cgcgccgaaa gtgtgcttga taaccaacgt cgccgtgagc
    76921 tttcgaatca aagctgggaa ctgatgaacc aactcatcgc tgaacctgct caaccgacgc
    76981 tcgccctcaa ggcgttaatg aaaaggaaaa acagcgatgg tcgacaagca tgaagagatt
    77041 actctgccca tagtcctctc ctgtaattat cagtctgata ttacttatcc tgggcaaaaa
    77101 cagtttgatt gcggtaaccc tgttatcgat aaatttgtac gcgcatcgct aaagaaaagt
    77161 gtgcgtaata gcgactgtgc ggctaaagca cttattgaca gacaaagtgg tgaactgatc
    77221 ggcatctgta cttttacggc atattcgctg gaaaaacaac gcgtttctgg cgtccttcag
    77281 ggttcacaac cttcagaaat tggtgttgtc agattagtca tgttgggggt agcacggaag
    77341 tatcaaaagc agggctttgg tcaggaccta ctatgtgatt tttttgaaca tgtaaaaata
    77401 attcaccagg cattaccaat taaaggggtt tatcttgatg ctgaccctgc cgccattaat
    77461 ttttatgctc gtctcggctt tgttcagctt tcagcgacac caaatgcttt tggtgctgta
    77521 cctatgtttt tggcgattca gcatattctc gcggcttaga ttattatcgc cacccgccag
    77581 ggcaagtttt gcctcagccg ggggctgagc agcgttacca cttttgttca attgcccccg
    77641 ctttcagcaa ctccacatcc ttgccgttaa tggttgccgt tgcggcaacg attgtggtcc
    77701 ttacagttgc acaagatttt cgctcaaatt ccggtttata aagaataaga gacgaattac
    77761 tggtaaatga accaaatagg taatcagtaa agctaatatt cagccggttt aaaccgaaag
    77821 gcgcgaagcc aaccgtttgg ttaatctcct taccggatgg gcgacgaatg ctgaatgtca
    77881 cgcgaagatt ggccacttcg cctaatttat tggtggtggc gaagaggcta tccatgcagg
    77941 aaaaaccttt atctaaaaaa ttatctcgca tcccagaacg ggtaaccgta atcacgggta
    78001 acgtatcgtc aaccgttttt ctgtcaatca tgtcttttcg acaagtagcc agaaagtctt
    78061 ccgggccacc ataaatgtgg acacctttat caagatattc cttcgctttc ttacaatcta
    78121 cctcaacata ctgaccattg gcgtacataa tgcccaacct ggaccatgct aaattcacac
    78181 cgtgctgcgc ggcctgatta aatagtgcat acgctttggc ataatccacc cgcacctgct
    78241 caccgtcgta gtagataccc cccagcgcta acggagcttc tctgtcacct tgtttagccg
    78301 ctaactcgaa ccatttaatc gcttcctgaa catcaggttt ggtatcataa cgaggctgat
    78361 aatattccca gcctaacagg ctctgcgcgt cgatatctcc cgattcagcc attttatgca
    78421 gtgtatttat aacatcggga gcaggtttac caccgtatat gctgtcatac aattcatgcg
    78481 ctttctcgct ggccaccacg cttggtgaaa ctatcagcat tacagaaaat aatattcttt
    78541 tagttaacat gtataccttt atgtcctcac tgtcatatat ttccataata attaaccacc
    78601 aaatatccat ttaatgaata aatacagtgc cataaaggtc caagtaataa gcacacattt
    78661 agcctgaaac attatcatcc atgtcaccaa atcgtttatt tagtgagata aaactagcat
    78721 attcgtaaga taaaaaatgc acatggcaat caagaaatga tattattact ttgttgatgt
    78781 aaaactattt agttttaaaa ttcatttttt caacatatag agcattcatt ttatgaatgc
    78841 aaaaaaacat acctgacaca caaattactc tcctctgtaa tattttctca ttaacactct
    78901 gtttacccaa catgcgctcc gcactacact atgtgggcat ttttttaagg ggtggtaatg
    78961 aacgattata aaatgacgcc aggtgagagg cgcgcgacct ggggtttagg gaccgtattc
    79021 tcgttgcgca tgctgggcat gtttatggtt ctgccggttc tgaccacgta cggcatggct
    79081 ctgcaaggtg ccagcgaagc attaatcggt attgccattg gtatttatgg tctgactcag
    79141 gccgtttttc agattccgtt tggcctgctt tccgaccgca ttggtcgcaa accattaatt
    79201 gtcggtgggc tggcggtgtt tgccgccggt agcgttatcg ctgcgctttc cgactccatc
    79261 tggggaatta ttctgggccg ggcgctacaa ggctctggag caattgccgc cgccgttatg
    79321 gcgctgcttt ccgatctcac gcgcgaacaa aaccgcacca aagcgatggc gtttatcggc
    79381 gtgagctttg gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa
    79441 cttgggctgc acgcgctgtt ctggatgatc gctattctgg caacaaccgg cattgcgttg
    79501 accatttggg ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg
    79561 aaaggcagtt tcagtaaagt gctggcggaa ccgcggctgc taaaactcaa ctttggcatt
    79621 atgtgtctgc acatgcttct gatgtctacg tttgttgccc tgcccggaca gctagctgat
    79681 gcggggttcc cggcggctga acactggaag gtctatctgg cgacgatgtt aatcgccttt
    79741 ggctcggtcg tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt
    79801 gtcttctgcg tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag
    79861 ttctggcaac tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc
    79921 cttctgccct cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt
    79981 gtctactcca ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt
    80041 gacggcatgt ttgacggtca gggggtattt ctcgctggcg caatgctggc cgcagtgtgg
    80101 ctggcagtcg ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc
    80161 ccggcggaca ttgccgcaaa cgaagcgtta aaagtgcgtt tgctggaaac tgaaggcgtc
    80221 aaagaagtgt tgattgcaga agaagagcat tcagcttatg tgaaaatcga cagcaaagtg
    80281 acaaatcgct ttgaggttga acaggcaatt cgtcaggcat aaaaaacgcg gcgaattgcc
    80341 tgctgcgcta cgcttaccag gcctacaaag ttcttgagtt acaaaccgtt gtaggccgaa
    80401 tcaggcgttc atgccgcatc tgacatgaac aaaacgcaca ttgtcgcgat taatcgcgga
    80461 agtttttgaa ctggaacggc tggccgagat cgccaccacg taccatcgcc atgacagcct
    80521 gcaaatcatc acgagatttg ccagtcacac ggatctcatc gccctgaatt tgcgcctgca
    80581 ctttcagttt gctgtcttta atcatcttga cgattttctt ctgggtcgcg ctctcaatgc
    80641 cctgtttcag tttcgcttcc acaaaccagg ttttaccgct atgaacgata ttttccggta
    80701 catccagtga actgccttca atgccgcgct tcagcagctt ggcacgcaga atatccagca
    80761 actgattgac ctggaagtcg gactcgctca acactttgat ggttttgctg gcgtcgttca
    80821 gctcaaatga ggcttcaacg ttacggaagt caaaacggga ctccacttcg cgactcgcgt
    80881 tatcgaccgc gttacgtgct tcctgaagat caacttcaga gacaatatcg aaagatggca
    80941 tcttttcctc tcccttcatt tttgatgcga agcataatac ctgcaaagtt aaataaccaa
    81001 cagtcctgca ccaggacgta tcccttaatt gtgcatagtc gcaacagcat gcacaattaa
    81061 gggatagcct tattgaacac ccggagtggt tgcgggtgag gaggaacaat gaaaattacc
    81121 gtattgggat gcggtgcctt agggcaatta tggcttacag cactttgtaa acagggtcac
    81181 gaagttcagg gctggctgcg cgtaccgcaa ccttattgta gtgtgaatct ggttgagact
    81241 gatggttcga tatttaacga atcgctgacc gccaacgatc ccgattttct tgccaccagc
    81301 gatctgctcc tggtgacgct gaaagcatgg caggtttacg atgccgtcaa aagcctcgcg
    81361 tccacactgc ctgtaaccac gccaatactg ttaattcaca acggcatggg caccatcgaa
    81421 gagttgcaaa acattcagca gccattactg atgggcacca ccacccatgc cgcccgccgc
    81481 gacggcaatg tcattattca tgtggcaaac ggaatcacgc atattggtcc ggcacggcaa
    81541 caggatggcg attacagtta tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc
    81601 tggcataaca atattcgcgc cgagctgtgg cgcaagctgg ccgtcaactg tgtgattaat
    81661 ccactgaccg ccatctggaa ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt
    81721 atgcagatat gcgaagaagt cgcggcagtg atcgagcgcg aagggcatca tacttcagca
    81781 gaagatttgc gtgattacgt gatgcaggtg attgatgcca cagcggaaaa tatctcgtcg
    81841 atgttgcagg atatccgcgc gctgcgccac accgaaatcg actatatcaa tggttttctc
    81901 ttacgccgcg cccgcgcgca tgggattgcc gtaccggaaa acacccgcct gtttgaaatg
    81961 gtaaaaagaa aggagagtga atatgagcgc atcggcactg gtttgcctcg cccctggtag
    82021 tgaagagact gaagccgtca ccactatcga tctgctggtt cgcggcggta tcaaagtcac
    82081 cactgccagc gtcgccagcg atggcaacct ggccattacc tgctcgcgcg gcgtgaagct
    82141 gctggcggat gcgccgctgg tcgaggtggc tgatggcgaa tatgacgtga tcgtactgcc
    82201 tggtggcatt aaaggcgcag agtgttttcg cgatagtacc ctgctggttg aaaccgttaa
    82261 acagttccac cgttccgggc gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct
    82321 ggtaccgcac gatatcttcc cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa
    82381 aattcccgcc gaacaatggc aggacaagcg cgtcgtctgg gatgcacggg taaaattgct
    82441 gaccagccag gggccgggta cggctattga ttttggtctg aaaattatcg acctgctggt
    82501 tgggcgcgag aaagcccatg aagtggcatc acaactggtg atggcggcag ggatttataa
    82561 ttattacgag tagtgtcgga tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac
    82621 cacaaattat gggcgataca ccttcacatt gttaaagccc tgctcgcgca gatagagcgc
    82681 ctgcagacgg ctcatcaccc cgcgctcaca ccacagtagc caggttctgt tctggtcgag
    82741 atcgccaaac ttggtgctca gtttatagaa cggcagagaa actacatcaa tcccttcgac
    82801 tttcagtggc ttatcttcct gttcatcgat agaacggata tcgaggatca cgtcgttcgg
    82861 gccgaagcca ttgacggttt ccacttccac cacttcctgc tcggtctgct gggcgatttc
    82921 gcggatatca acgttattcg cttcttcaac cactttatcg agaatgctga agtcgaattt
    82981 ctcttcttcc gcttcaatct tcgacttaac cgctttcacc gtcgggcttt tggagataac
    83041 accgcaatat tccggcatcg tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt
    83101 gatgatgtgc tctttgtcgt aagaaatcag cggacgcagg atcagcgtat cggagacgtt
    83161 atcaatcagg cgcaggttgg tcagcgtctg actggacacc tggccgagcg cttcgccggt
    83221 gaccagcgcc tgtacgccgt aacgttcagc cactttagac gcggcacgca ccatcatacg
    83281 tttgaggata acgcccatct gaccgtcgtc gattttctcg agaatttccc cgacgaccgg
    83341 ttcgaaatta atagcgacaa aacgcacgcg gtgggagctg ccaaaacggt tccacagata
    83401 atgcgccacc tgacgcacgc caatttcatg cgccgcgccg ccaaggttaa agaaacagta
    83461 atgcacgcgg cagccgcgac gcatcaacat ataactggaa acaccggagt cgaaaccacc
    83521 ggaaatgagc gacagcacat cttcctgggt gccgatcggg aaaccgccaa taccttcgta
    83581 gcggccttta atcagcagga gacgatcgtc ttccacttcc agatggacag tcacttccgg
    83641 attggtcagc ttcacgcgcg cggactcaat atgctgattt aaaccgccgc cgacgtaacg
    83701 ttccacatca atcgagctaa aatcatgttt gccacgacgc ttcacgcgta cgcagaaggt
    83761 tttgccttcc agctgatcgc gatactgaac caacgctttc tcaaaaatat cgtgcatgtc
    83821 ggtaaacggc acgtcttcga cttcgagaat atggtggata cccggaatac gggtcagggc
    83881 gtcgcgaata gccagacgct ggttttcatc ttttgcgcga acttcgatgt tatcccagtg
    83941 gcggacgaca gcgagcgtct catcatagtg ctttaaaacg ttacgaatgt tcccggtaag
    84001 gatttttata aagcgcaagc gcacagattg gcttttgatg gtgatttccg ggaacaattt
    84061 aatgataaac ttcatggcgg caatggttcg ttggcaagcc ttaagcgact tgtataggga
    84121 aaaatacagc agcccacacc tgcggctgca tccaggcgcg gaagtatacc actaacatcg
    84181 ctttgctgcg cacatcacct taccattgcg cgttatttgc tatttgccct gagtccgtta
    84241 ccatgacggg gcgaaaaata ttgagagtca gacattcatt atgccgaaga aaaatgaggc
    84301 tcccgccagc tttgaaaagg cgctgagcga gctggaacag attgtaaccc gtctggaaag
    84361 tggcgacctg ccgctggaag aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg
    84421 tcaggggcag gccaaattac aacaagccga acagcgcgta caaattctgc tgtctgacaa
    84481 tgaagacgcc tctctaaccc cttttacacc ggacaatgag taatggactt tccgcagcaa
    84541 ctcgaagcct gcgttaagca ggccaaccaa gcgctgagcc gttttatcgc cccactgccc
    84601 tttcagaaca ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgc
    84661 ctgcgacctt ttctggttta tgccaccggt catatgtttg gcgttagcac aaacacgctg
    84721 gacgcacccg ctgccgccgt agagtgtatc cacgcttact cattaattca tgatgattta
    84781 ccggcgatgg atgatgacga tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc
    84841 gaagcaaacg cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattctaagc
    84901 gatgccaata tgccggaagt gtcggatcgc gacagaattt cgatgatttc tgaactggcg
    84961 agcgccagcg gtattgccgg aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc
    85021 aaacacgtac ctctggacgc gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt
    85081 cgcgccgccg ttcgccttgg tgcattaagc gccggagata aagggcgtcg tgctctgcca
    85141 gtactcgaca agtacgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg
    85201 gatgtggtag gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt
    85261 aaaagtacct accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg
    85321 atcgacgatg cccgccagtc gctgaaacaa ctggctgaac aatcactcga tacctcggca
    85381 ctggaagcgc tagcggacta catcatccag cgtaataaat aaacaataag tattaatagg
    85441 cccctgatga gttttgatat tgccaaatac ccgaccctgg cactggtcga ctccacccag
    85501 gagttacgac tgttgccgaa agagagttta ccgaaactct gcgacgaact gcgccgctat
    85561 ttactcgaca gcgtgagccg ttccagcggg cacttcgcct ccgggctggg cacggtcgaa
    85621 ctgaccgtgg cgctgcacta tgtctacaac accccgtttg accaattgat ttgggatgtg
    85681 gggcatcagg cttatccgca taaaattttg accggacgcc gcgacaaaat cggcaccatc
    85741 cgtcagaaag gcggcctgca cccgttcccg tggcgcggcg aaagcgaata tgacgtatta
    85801 agcgtcggtc attcatcaac ctccatcagt gccggaattg gtattgcggt tgctgccgag
    85861 aaagaaggca aaaatcgccg caccgtctgt gtcattggcg atggcgcgat taccgctggc
    85921 atggcgtttg aagcgatgaa tcacgcgggc gatatccgtc ctgatatgct ggtggtcctc
    85981 aacgacaatg aaatgtcgat ttccgaaaat gtcggcgcgc tcaataacca tctggcacag
    86041 ctgctttccg gtaagcttta ctcttcgctg cgcgaaggcg ggaaaaaagt tttctctggc
    86101 gttccgccaa ttaaagagct gctcaaacgt accgaagaac atattaaagg catggtagtg
    86161 cctggcacgt tgtttgaaga gctgggcttt aactacatcg gcccggttga cggtcacgat
    86221 gtgctggggc ttatcaccac gctgaagaac atgcgcgacc tgaaaggccc gcagttcctg
    86281 catatcatga ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc catcactttc
    86341 cacgccgtgc ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggtttg
    86401 ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag
    86461 ctgatggcga ttactccggc gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa
    86521 ttcccggatc gttacttcga cgtggcaatc gccgagcaac acgcggtgac ctttgccgcc
    86581 ggtctggcga ttggtgggta caaacccatt gtcgcgattt actccacttt cctgcaacgc
    86641 gcctatgatc aggtgctgca tgacgtggcg attcaaaagc tcccggtcct gttcgccatc
    86701 gaccgcgcgg gcattgttgg tgctgacggt caaacccatc agggcgcttt tgacctctct
    86761 tacctgcgct gcataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc
    86821 cagatgctct ataccggcta tcactataac gatggcccgt cagcggtgcg ctacccgcgt
    86881 ggcaacgcgg tcggcgtgga actgacgccg ctggaaaaac taccaattgg caaaggcatt
    86941 gtgaagcgtc gtggcgagaa actggcgatc cttaactttg gtacgctgat gccagaagcg
    87001 gcgaaagtcg ccgaatcgct gaacgccacg ctggtcgata tgcgttttgt gaaaccgctt
    87061 gatgaagcgt taattctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa
    87121 aacgccatta tgggcggcgc aggcagcggc gtgaacgaag tgctaatggc ccatcgtaaa
    87181 ccagtacccg tgctgaacat tggcctgcct gacttcttta ttccgcaagg aactcaggaa
    87241 gaaatgcgcg ccgaactcgg cctcgatgcc gccggtatgg aagccaaaat caaggcctgg
    87301 ctggcataat ccctactcca ctcctgctat gcttaacaaa ttattcatag actctaaata
    87361 attcgagttg caggaaggcg gcaaacgagt gaagccccag gagcttacat aagtaagtga
    87421 ctggggtgaa cgaatgcagc cgcagcacat gcaacttgaa gtatgacgag tatggcagga
    87481 gtggcagcat gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc
    87541 tcggctgtat gacctttggc gagccagatc gtggtaatca cgcatggaca ctgccggaag
    87601 agagtagccg tcccatcatt aaacgcgcgc tggaaggcgg cataaatttc tttgataccg
    87661 ccaatagcta ttccgatggc agcagcgaag agatcgttgg tcgcgcactg cgggatttcg
    87721 cccgtcgtga agacgtggtc gttgcgacga aagtgttcca tcgcgttggt gatttaccgg
    87781 aaggattatc ccgtgcacaa attttgcgct ctatcgacga cagcctgcgc cgtctcggca
    87841 tggattatgt cgatatcctg caaattcatc gctgggatta caacacgccg atcgaagaga
    87901 cgctggaagc cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat
    87961 ccatgcacgc ttcgcagttt gctcaggccc tgaagctaca aaaacagcac ggctgggcgc
    88021 agtttatcag tatgcaggat cactacaatc tgatttatcg cgaagaagag cgcgagatgc
    88081 tgcctctgtg ttatcaggag ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc
    88141 gactgacgcg tccgtgggga gaaactaccg cacgactggt gtctgatgag gtggggagaa
    88201 atctctataa agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca
    88261 gtgaagaact cggtgcaaca cgagcacaag ttgcgctggc ctggttgttg agtaaaccgg
    88321 gcattgccgc accgattatc ggtacatcgc gggaagaaca gcttgatgag ctgctgaacg
    88381 cggtggatat cactttgaag ccagaacaga ttgccgaact ggaaacgccg tataaaccgc
    88441 atgcggtcgt aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc
    88501 aggcatcggt gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca
    88561 ggatgcctgc ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat
    88621 gtacattgcg atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa
    88681 tcacaaaccc ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc
    88741 acataccaat aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg
    88801 ccgtttgatg acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga
    88861 gctgccaggg taaaaaggtc atcagatacc agaacggaat cgctgccagc gatcccatcg
    88921 tgccaggaac gattgggctt aatccacttc cgaatccgac agcaagcaga tgccacggat
    88981 tactcatctt caggcgactt ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct
    89041 ttccagtcaa acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg
    89101 gtcatttgcc cgatacaggt aaacggtacg cccaggtgtc cgagagccac atccagcgcg
    89161 ccacggttca gttccggcac agtgaaacac aactcgtaat cttcaccgcc agagagcgcc
    89221 cagcgcagcg cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat
    89281 gccaggtcaa tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc
    89341 agaccgtcag agagatcgat agctgaattt gccagatcgc gcagtgcctg tccctgtaaa
    89401 atacgcggcg atggacggag atgacgtttg atcaagtagt ccgcatcttt agcatcggca
    89461 acctgcaaac ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc
    89521 acatagatcc agtcacccgg tttcgctcca gagcgcgtta aggctcgtcc catcggaaca
    89581 aagccgtgga tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt
    89641 tgcatatcgt aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct
    89701 tcgtctacgt ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct
    89761 gccagatcgc ttaggttcac cgccagtgct ttatacgcca gatcagcagg atcgatatca
    89821 tggaggaagt ggttgcccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg
    89881 gggatattga gaagagcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt
    89941 cttacacggt caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc
    90001 tcagcaggta aaaaagaaaa ggccggcgat cgcggaacgg tcttccgtga atctaccggc
    90061 ctggatatca ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga
    90121 cgaacttatg gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg
    90181 ccactttgta tggcacatcg ctacgtttag acagctcgta cagcgcaatg cgcagtactg
    90241 ctttttctac ctgtcccagt tcttccagca ggcgggacag gtatggcttc atcagtccgt
    90301 cgaggtatgc ggtgttagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa
    90361 cgtcttttac atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg
    90421 acaactgcca ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag
    90481 gtttcacgga tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt
    90541 tcaagcgcgg tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg
    90601 atcgcttgtt caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc
    90661 tgggcaacat gcgccaggcc gttgcttgca ccaccagcga catattcaaa gtgggcagtg
    90721 ccaccacgaa taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt
    90781 gcacccgccg ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca
    90841 tcttttacct gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg
    90901 ataaagttgt tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta
    90961 gcttcaataa tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat
    91021 tttagcataa tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa
    91081 atgcaggcaa acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc
    91141 ggctaatttc tcaagccctg gcagcgagca taatccacgg gcgtcgctgc ctaatagttt
    91201 aggtgcgata tagacaatca gctcatcgac taaacccacc tgcagcaatg cgccagcgag
    91261 cgttggcccc gcttccaccc agatgctgtt aatttgctgt ttacccagtt gcatcatcag
    91321 tacaaccaga tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg
    91381 ccactcacga gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat
    91441 gcgatgttcc ggcgtcacgc gattttggct atcaatcaca atacgtaccg gctgacggag
    91501 attttgttgc gggtagagca cctgagtttg ttcatccagt tcggaccaac gcaccgttaa
    91561 ggcaggatca tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgcgcgcg
    91621 cagtcgttgt acgtcgcgcc gcgcctgagt tgaagtgatc cactggcttt cgccgctcgc
    91681 catcgccgtg cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt
    91741 gcgcatccgc ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg
    91801 gctgacgtca atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt
    91861 aggatcttgc atcgctgcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca
    91921 cggtggcgta cgaccatgat ggctacaggg ttcgagtgtg acataggcgg tcgcaccttt
    91981 ggctttttca cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg
    92041 gtggtaacct tcaccgacaa tttcgccatc tttgacaatg acgcacccga cattcgggtt
    92101 gggatgcgtg gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc
    92161 gtcctgcacg gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc
    92221 gaaactgcgg tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg
    92281 ctccatcacc agattgccaa tcatcttgct cggtacttcg cgctcaccgg tggcgcgcaa
    92341 ctgcgattta atatgattga ttgccatttc aacgtcatcg gaactcaccg gacgtttttc
    92401 cagcgcccgc agcattccgc tgcgcaattt ctcttcatta aacggttcac gcacgtcgtt
    92461 gctttttaca acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt
    92521 acacaccaga cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc
    92581 aattacctta gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt
    92641 ggttaacgga aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga
    92701 gataaaggat cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga
    92761 aagggacgac ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc
    92821 cttagtgcct gtgtgcagca aagcgaagtg cgacagatga aacacagcgt cagcgcgctg
    92881 aaccaggaga tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg
    92941 aatgcaaaat ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg
    93001 aaaagccaga tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc
    93061 accaccctga cgctacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga
    93121 actgttgaat atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcaa
    93181 aatcaattga ttaacgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta
    93241 cagttaaagg ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct
    93301 gttatgcagt aaacgtattt cggggcgatt ttgtgcgtcc cgcaacatct ttccccgtca
    93361 ttttgttact ctgcttacat cacccagatt gatagtaaaa gtttgcaaca agggtgaaag
    93421 tcagtacaat ccctgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata
    93481 gaactgtgaa acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat
    93541 acgaaatatt tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag
    93601 tggcatacat atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc
    93661 ttttactgtc aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca
    93721 gagcgttaac gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaatgatac
    93781 ctaccttgag tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga
    93841 tgcgccggta ttcttcggcg gtaactccga tgcaaaaggt atctggaacc acggttctcc
    93901 gctgtttatg gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag
    93961 cttcggtccg ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa
    94021 taaagatggt cgccagagca cctggtacat gggtctgggt accgacatcg atactggtct
    94081 gccgatgagc ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc
    94141 gaacgaaaac gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga
    94201 tctgtggggc ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt
    94261 gggggatgac agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc
    94321 ttccagccat attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta
    94381 ctggcacgac ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt
    94441 caacgttcgc tctaccggct ggggtggtta cctggttgta ggttacaact tctgattatg
    94501 aaaatgccgg gatttattcc cggcatttca gattgttaac aacgtgcgct ttgttcatgc
    94561 cggatgcggc gtaaacgctt tgtccgaccc acaaaagcac gtaaattcaa tatattgctg
    94621 aaataacgta gtcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt
    94681 cttgttcatc atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt
    94741 ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga
    94801 atgctcatga tcgtggcaat agagacaaag caattcccag ttactgccat cttccgggtt
    94861 attggtatgg tcgtgatcaa tgtggtgaac agtaagttca cgcaggttgg aatagacaaa
    94921 ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc
    94981 gctttctaac cgcgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat
    95041 gtagggttac gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa
    95101 aaatcccgat cttctgaccg ggattttcaa catcaacggg aacttgatta aggcagaatt
    95161 gacggctgat ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata
    95221 cccagtttca gagccaacgc agatgccaca tagatggaag atgcagtacc gatggaaaca
    95281 ccgataagca tggtcagcga gaagccttcc agtaccggac caccgaagag gtacagcatc
    95341 aggataacca tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag
    95401 gacacgttaa agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaata
    95461 cggtccgata ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc
    95521 acaatggtca ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg
    95581 tcgtgcgcca gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg
    95641 tacacgagga tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtct
    95701 gcccccacgc tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat
    95761 tcgttaatca ccttcagaac ctggctgccc agcacctgac cgccggtttc gccttcagca
    95821 ggcggcatac ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca
    95881 aaaccggctt tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta
    95941 atttcgataa ccgtaccacc ggtaaaatcc agcccccagt taaagccgcg cacgcccata
    96001 ataacgatag cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc
    96061 ataaagtcat agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca
    96121 tcgcactcct cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat
    96181 ggcacgcgta ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat
    96241 cgcgaaccct ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt
    96301 ggtgatgttc gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg
    96361 ttgaacagta cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc
    96421 atcgaccgcc accgcaaggg ttaagacgat acccgcgata cctggcatac tcagcgttgc
    96481 gcctggcaac agcgacataa tgccgacgat taagatcaag ttggcaatca gagcactggt
    96541 cgcaatcaga ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc
    96601 ggcaaggcaa gcctccagcc cctgttcaat gttctgcata cccagggttg ggccaatggt
    96661 acgttcttca acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag
    96721 ctgacgggct tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga
    96781 ctggatgttg gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc
    96841 atctttctta ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt
    96901 gtccttagtg aagttagaca tgatgttacc accagcgcta tcgagcgaga tgttaacctg
    96961 cggttggttg tattcgtcct ggctggaagt ggagtcggtg atatggtcac cggtcagaat
    97021 tacgcgtttg tacagcacaa ctggctgacc ttcgcgggtc tgtttcactt cagagtcgcc
    97081 cggtacgcga ccggatgccg cagcggcctg gtcaacgttg gtgtttacca gacggaattc
    97141 cagcgttgcc gtcgcaccca gaatctcttt cgcacgcgca gtgtcctgaa tacctggcag
    97201 ttcaacaacg atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg
    97261 gtttacacgg ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact
    97321 cagacgagca tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag
    97381 gtccggatgg cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa
    97441 agtaatgctc aggccgtagt tgttttcttt acgaacggtg gtatacggga tgcccttttc
    97501 acgcagatcg ctgcgcaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc
    97561 catatccact tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg
    97621 ctcagcgtga atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac
    97681 gtatttgtca cccataacgc ccattaatgc ttcacgagcg cgcagctggg tgtcagtgga
    97741 gtcgaagcgc gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc
    97801 ttcttgtaac gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc
    97861 accagtgatc tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc
    97921 gatgacaatc accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc
    97981 aattccctta gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc
    98041 agctacgaag tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta
    98101 gccgttttcc gctactttgg ttacgcggcc aaccaggcca ccgttcgtca gaacttcatc
    98161 acctttggca atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg
    98221 caggatcatg aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg
    98281 gctaccttgc gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa
    98341 atttccctca ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct
    98401 ggtaaaaatc agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg
    98461 ccatcaaacg ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt
    98521 cgttgcaacg gtcaagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat
    98581 cacactcagg atcgagtggg ccagtatcgc tcttatactt cgcattgcgg attttcacca
    98641 cgccatcggt cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa
    98701 acatatcgat accacgacgt acgccttcaa ccaggtcttc tggtttacca acgcccatca
    98761 ggtaacgcgg tttgtctgcc ggaatttgcg ggcatacatg ctccagaatg cggtgcatat
    98821 ctgctttcgg ctcacccaca gccagaccgc cgacagcgta gccatcaaaa ccgatatcta
    98881 ccagaccttt aacagaaata tcacgtaaat cttcgtaaac gctgccctga atgataccaa
    98941 acagcgcatt tttgtttccg agactgtcaa aacgctcacg gctacgcttc gcccaacgca
    99001 gagacatctc catggagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat
    99061 caaagatcat gacgatatcc gaaccaagat cgtactgaat ctccattgat ttttcaggat
    99121 cgaggaaaat cggatcgccg ttgatcgggt tacggaagtg cacgccctgt tcggtgattt
    99181 tacgaatatc gccaaggctg aagacctgga aaccgccgga gtcggtgagg atcggcccct
    99241 tccactgcat aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca
    99301 gccacaggtg gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg
    99361 gcgtcatccc ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta
    99421 cgccacgatc aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa
    99481 atttcatttt ttctccacta cgtcagaaaa acagtccaac gttttaaacc agcgccgcgg
    99541 aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca
    99601 ccgtaactaa aaaagcgata tttctcttct accgctgctt tataggcgtt catggtgtgt
    99661 tgataaccgg caaaggccga aaccagcata atcagcgtcg actctggcaa gtggaagttc
    99721 gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat ttgggtatcg
    99781 tcgaagaacg gttcaatgag atcgtttttc gctgcctgag ccgcgctttc cagcgaacgt
    99841 actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg
    99901 tctaccacat cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg
    99961 tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc
   100021 acgcctttgg cgcgcaattt ttccagcaaa ggctcgtcaa aatgcagacc agcggtcggg
   100081 gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct
   100141 tcgtccggac ggtcgatata cggcggcagc ggcatatggc cgatactgtt gaggatatcc
   100201 agtaccgaac gatcatcatt gaattcgact tcaaacagtg cgccgtggcg cgcggtcatt
   100261 gttgcgttaa tactttcgtc atcgcccagc agcagttctg cgccaggttt tggcgctttc
   100321 gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac cagcacttca
   100381 atcttgccgc cgctggcttt acgcccaaac aggcgcgccg ggatcacgcg ggtattatta
   100441 aaaaccagaa gatcgccggg gttgagctta tcaagtaaat cggtgaaagt accgtgcgtc
   100501 agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatggga
   100561 tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg
   100621 actcagacta aaataagagg cgggtagtct agtgccgggg cgctctccct gcaaccttta
   100681 cccccttttg gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct
   100741 cgcggaaagc tcgctttccg gcaatttact ggctgatttc gtacgcggaa atcctgaaga
   100801 aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtatcg acgtattaac
   100861 cgacaatctg ccggaagtcc gcgaagcacg ggaatggttt cgtagtgaaa ctcgccgcgt
   100921 tgcgcctatt acgctggatg tcatgtggga tcactttctt tcccgccact ggtcgcagct
   100981 gtcgccggac tttccgctac aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat
   101041 tttgccggac tcaccgccac gttttatcaa tctgaacaat tacttgtggt cagagcagtg
   101101 gctggtgcgc tatcgcgata tggatttcat ccagaacgtg ttaaacggca tggcaagccg
   101161 ccgcccacgt ctggatgccc tgcgtgactc ctggtacgat ttagacgctc attatgacgc
   101221 cctcgaaacc cgcttctggc agttttatcc gcggatgatg gcgcaggcgt cacacaaggc
   101281 gttataaaac gttagttcat ccataccgta gccgaaatgg caggcaaagc cagaatcccg
   101341 tttgtcagtt gcccatggcc ttctttgcgt tgccattgcg cgacattaag aaacggtgac
   101401 gcggggagca ccacttcaca ggcctcgcca cggttgattg ccaccagtac acgttgctga
   101461 ttcagcacgc ggacaaatac cactacgtta tcttccgcat acagcacctg acagccgcca
   101521 cgacgcagcg cctgactttt cttacgcagc gcaatcattc gctggtacag cgcgaataac
   101581 gccgtatcct gcttttccac ctgccagggg aacggtttac ggcaaaacgg atcgtttttg
   101641 ccatccagtc ctacttcatc accgtaataa atgcacggta caccaggcca ggtgaacagc
   101701 cagaccaccg ccagcggcag gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca
   101761 gtatcgtggc tgtcgagctg attaaacata cgtaattgtt gttgatgaga aagccctgcg
   101821 cggtaattat ccatccaggc catacaggtt tgggcatcaa tttgctgcgg atcgtaagag
   101881 atatcggtat tggcaagaaa tccccacaac gggaatgtga agccacgata gttcatggcg
   101941 gcatcttcca catcggcctg taaccattgc cgtgcatcgc caaaatgttc gccgacaata
   102001 tacgcttccg gctgggtttc tttcgccgct tcggtgatcc cagcaacgtg ctgcatatta
   102061 ttgcgcgccc cacccgcctc ccccagcata tgcaccacat ccagccgcca gccgtccata
   102121 ctccacggcg ctttcagcca atggcggaca atactgtctt ccccgcgata aatttcattc
   102181 accagacttt ccgactgata atccagcttc ggcaagctgg catagccaag ccagtcgagc
   102241 gccgtgccat catcactaaa cgagtaccag tcgcgccagg gcgattcagg gttgtgacaa
   102301 gctccccccg tgccacgatt gtgcctgtca aaccaggcat gggaatcgcc actgtggtta
   102361 aacacgccat ccagcaccag ccgcattccc agctgctgcg tattgtggcg taagcgcagc
   102421 aacgccccat caccgccaaa ctgcggatcg acatggcgat aatcctcggt atcgtattta
   102481 tgtacgctgg gagctttaaa caccggattg agatacagcg ccgtcacgcc aagctttttc
   102541 agatacggca gtttttcgct tatcccgtcc agatcgccgc catagaacgt tgatccgccc
   102601 gcctgcgccg tgaccggttc atcccagtca cgcaagatga tcttttgtcc ggctgcatga
   102661 tggtaataga catgatcctg ttcagcttca cgaggaagac tacgcgcaaa acgatcaggg
   102721 aagatctgat aaaaaatctg atccgcagcc cattgtgggc cgatatccgg tacatcgacg
   102781 gcaaactgct ccagtcgtgc cggcggcatt cggctgaagc cctgcggtgt aaaccaacgc
   102841 tggcgatcgt gccacagcag tttgaaactg taacgccgcc ggggttgtcc gctggagaga
   102901 tcaatcgccg cacgccatgc ggtgacgcca ggctgctgct gactgcgctg cttatgcatc
   102961 gatactgaca tttcttcgtt atcgtgttct gtacgcagca taatgcgctg cggtgggtct
   103021 tcgcccgtca gccacagtgt aatgagcagt tgatctttgc tttgtttaac aaatgggggc
   103081 accggcaggt gccatgcatt taacatcata attcccctgt gatgaaaatc tgatcacctt
   103141 gccatagcct aatgcaggat cactcatcat ctgaaggata tttgggggag gaggacgggg
   103201 gcggataact ttcggaatag ccggatcagg atttcctcct gaaccggctt acagaacgca
   103261 aggattactg gttttcagcc agctgacgat cgtggcggcg tttaaacatc cagccaatca
   103321 acagcacaac aatccacgcg aaaccgacat acagcgagat acgcgtatcc gggtgataac
   103381 caatcaaccc gataataaag agcaggaaaa tcaaaccgcc gatggtcgtt gctaccccac
   103441 ccggcacttt aaatttcagc gccttaactt cttctggcgg caaacggcga cggaaggcaa
   103501 tttgcgacag caggatcata atccacaccc acaccgtggc gaaggttgcc agcgaagcga
   103561 tcaccaggaa gacgttttcc ggcatgatgt agttcagata caccgcaaac agcagcgcgg
   103621 tagtcatcac cagcaccgta acccacggaa taccgcgacg cgacgttttg ctaaaaattt
   103681 tcggcgcgct gccctgctct gccataccgt ggagcatacg gcctacgcca aatacgtcac
   103741 tgttaattgc cgacagcgaa gcagtcagca caacaaagtt aagaatgctg gcggcaaagg
   103801 taatgcccat atgctggaac gtcagcacga acgggctacc ggcagtgcca acctgattcc
   103861 acgggtagat agacataatg acgaacagcg taccgacgta gaacaccaga atacgcatcg
   103921 gcacggagtt aatcgcacgc ggtatcgatt tctcaggatc tttcgcttca ccggcggtaa
   103981 tcccgataat ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac atcaccatcc
   104041 caagccagcc gttactgaag aagccgccgt tgctccacag gttatgaata ccggtcggtt
   104101 gcccgccgtt gccaatcccc cagatgatga tgccgaaacc ggcgacaatc atgatgatga
   104161 tggtggcgac tttaaagaac gagaaccaga attccagctc accgaagacc ttcacgctca
   104221 tcaggtttac ggcgcaaatg atcagcacca cgctcagtac ccaaatccag tgcggcaccg
   104281 tcgggaacca gacacccata tagataccaa aagcggtcac atcggcgatg gcgacaataa
   104341 ggatttcaaa gcaataggtc cagccggtaa tgtaacctgc cagcgggccg aggttttcct
   104401 gcgcataacg cgagaaagag ctggctgccg ggttatgtac cgacatttcc cccagcgcac
   104461 gcataatgat atacgccgcg ataccaccga taatataggc caacaacacg ctcggaccgg
   104521 ccattttgat ggcgtctgcc gaaccgtaaa acagcccggt gccaattgct gaacccagtg
   104581 ccataaagcg tatgtggcgg gtacttagcc cacgctttag cttgttctta ctttccatca
   104641 attcctaacc catcaacaca ataaaaaacc acggggcatc gcagccccgt ggttaaaaca
   104701 aatgttcagt gatttagtga gcgctggagg tcacctgacg acctgccgcg cgatcccaga
   104761 taatggccag aaccaccatc accactgttg gcattaacca cgccagacct tgttcggcca
   104821 gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt
   104881 cgagaatacc aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg gacgaattat
   104941 gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga taaatagcgg
   105001 tcagcaccgg tacggagatc tggatcagct ggcttaagcc gaggttagaa accaccatcg
   105061 agaagccgcc gaggataaac accagcgtac gataagagag cggtacgtac tgggcaaaga
   105121 attctgcaca agcacaggtc aggccaactg ccgttaccag gcaggcgatg aagattaacg
   105181 ccgccagcag gaagctaccg ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag
   105241 cagcgccgtt tgcagactga tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg
   105301 ccaggtacag cagagtcaga ccaacacccg ccatcaggcc agcccagacg gtataacggg
   105361 tcagcagacg cgcttcggta acgccacgag aacgcgccgc gttaacaata acgataccaa
   105421 acaccattgc gcccagcgta tccatggtca gatagccgtt aacgaagccg ttagaaaacg
   105481 cagcgttttg ataagcctca gtcgccgtgc tgatagaacc cgccggccag acaatcgcgg
   105541 caacagacag gatgaccagc gcgataattt tcagcggcgc aaggaagttg cccacggtat
   105601 ccagcagctt gcccggatag agcgaaacca gaataacgat agcgaaatag accaggctgt
   105661 agataaacag cggcagcgcg gaatcaccag tcagcggcgc aatccccact tcaaaggaaa
   105721 cggtagctgt acgcggcgta gcgaaaagcg gccccaccgc caggtaacaa accgttgcca
   105781 gcagtacgcc agcgacttta ccgattggcg tgctgaggct gtcaacaccg ccgccaactt
   105841 ttgccagcgc cactaccgtt agtaccggca gaccaacggc agtaatgagg aagccgaatg
   105901 ccgcagtcca gacgtgttcg cctgcctgca agccgaccat tggagggaaa ataatgttac
   105961 ctgcgccgac gaacaacgca aatgtcataa agcccagagc gatgatatcg cgcgatctta
   106021 attgatgggt cataaatcat actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg
   106081 ccaatccctg gggatgatac agcggtatat ttaaacaatt ttagcgggaa atactcccaa
   106141 ccaggcgcgg cgaagaatag tttttcgatt taccgcgtta atgcagtcag tatgacagca
   106201 cctgaagcgc aatttaaacg cttataacgt tttaaagcaa ggagggatct aaaaaccaga
   106261 ggtatatagc gataagaaat caccaaccag tataaaacac tatttccgtg aaaaagacat
   106321 ggctaatcat gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc gtattttgac
   106381 tgcaaagaaa ataagccagc atcggctggc ttatggcaat aaaagatgac aaaggcggat
   106441 taatcgctgt ttttggcaat taaacgttcc gggataacaa aactgaaacg tgttcctttt
   106501 cctactgtac tctcaatatt caggcgactt tcgtgatgat tcaccgcatg tttcacgatc
   106561 gctaacccta atccgctacc gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa
   106621 cgctcggtca ggcgcggaat atgctccggt gcaatgcccg gtccgttatc ttcaacgcta
   106681 aattcggcac cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc ttctggagta
   106741 tgattcacgg cgttatagac caggttcgaa atcgcactgc gtagctgatc ttcgttgcca
   106801 gacactttga ggccgttatc tatctcaaag gtaaatgtct gttttttctg actcagagtc
   106861 tgagcctcac gctcaacaac gcgcagcatc atcggcacat caaccttttc attgagcaaa
   106921 tgcgtcggtg cggcttctat tttcgacagc gtcagcaact gcttcaccag cccttccatc
   106981 cgctgggtct gctcgcgcat ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc
   107041 tgctcatcca tcatctccag gtaaccctgt aacacggtca atggcgtacg taactcatgg
   107101 ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt gatgcatttg cgtgacatca
   107161 cgcgccacca tcagcaactg tttgtgggta taaggcatga cgcgaatttc cagatgccgc
   107221 ccggtgttga gcaccagatt gagcgggcga gaaaaatcac gcgttttcag atattgcgta
   107281 aactccgggt aacgcagcag gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa
   107341 ccaagaattt gttgcgccag accgttacac cagaaaatac cgccctcttc agtggtcagc
   107401 accactgcgt cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag attgcccagt
   107461 tcacggcggc gttttttatt tcgcagctgc atctggtgta agccgtatag tagcggttcc
   107521 cagctaccac gccccggtgg cggggtcata ctacgatcca cccacagcca ccatgaaagg
   107581 cgcaataaat tccagaaatg ccagataagc agtcccgtta ccgatgccag caaaaaccag
   107641 ggcaggtaac caaaaaatgc acccaggatg aaagccggga agcagcaaag tagcagctcc
   107701 agcaccagcc ttttccacga cagccgttcc agcacgtaag atactccagt taagaaatca
   107761 taagccctgc tctgcgtccg atgagcaagg cgttaaaagc gggttgaaaa acgatatcct
   107821 gtaccgcgca cggtctgcac catgcggtca tgcccgcctg gctccagtgc tttacgtagg
   107881 cgacgaatgt ggacatcgac cgtacgatct tccacataca cgttagttcc ccagacgtgg
   107941 tttaacagct gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt
   108001 ttaaattctg tcggccccat ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc
   108061 gggtcgagac ttaatccctg catctcaatc acctcttcca ccgccattgg cgaaatacgg
   108121 cgcattaccg ctttgattcg cgccaccagc tccttcggcg aaaacggctt ggtgatatag
   108181 tcatccgcgc cggtttcaag gccgcgcacg cgatcttctt cttcccctct ggcggtcaac
   108241 atcaccactg gaatatcccg ggtcatcgac tcgcgcttga ggtgtttgat gaactggata
   108301 ccggagccgc caggtaacat ccagtcaagg agaattaaat ccggccaggg ttcattcagt
   108361 tgattcacag cactgtcata atcttccgct tcgaccggct gaaagccatt ttgttcgagc
   108421 acgaagcaga ccatttcgcg aattggagct tcatcttcta cgaccagaat acgtctcgcc
   108481 atgatttgcc ctgttgtaat aaataggttg cgatcattaa tgcgacgtca ttatgcgtca
   108541 gatttatgac agatttatga aaagctcgtc gcacatatct tcaggttatt gatttccgtg
   108601 gcgcagaaaa aagcaaatgg cacatctgtt tgggtataat cgcgcccatg ctttttcgcc
   108661 agggaatcgt tatgcgcatc cttcacacct cagactggca tctcggccag aacttctaca
   108721 gtaaaagccg cgaagctgaa catcaggctt ttcttgactg gctgctggaa acagcacaaa
   108781 cccatcaggt ggatgcgatt attgttgctg gtgatgtttt cgataccggc tcgccgccca
   108841 gttacgcccg cacgttatac aaccgttttg ttgtcaattt acagcaaact ggctgtcatc
   108901 tggtggtact ggcaggaaac catgactcgg tcgccacgct gaatgaatcg cgcgatatca
   108961 tggcgttcct caatacgacc gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc
   109021 gtcgcgacgg gacgccaggc gcagtgctgt gccccattcc gtttttacgt ccgcgtgaca
   109081 ttattaccag ccaggcgggg cttaacggta ttgaaaaaca gcagcattta ctggcagcga
   109141 ttaccgatta ttaccaacaa cactatgccg atgcctgcaa actgcgcggc gatcagcctc
   109201 tgcccatcat cgccacggga cacttaacga ccgtgggtgc cagtaaaagt gacgccgtgc
   109261 gtgacattta tattggcacg ctggacgcgt ttccagcaca aaactttcca ccagccgact
   109321 acatcgcgct cgggcatatt caccgcgcac agattattgg cggcatggaa catgttcgct
   109381 attgcggctc tcccattcca ctgagttttg atgaatgcgg aaagagtaaa tatgtccatc
   109441 tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta ccggtaacgc
   109501 aacccatggc ggtgctgaaa ggcgatctgg cgtcgattac cgcacagctg gaacagtggc
   109561 gcgatgtatc acaggagccg cctgtctggc tggatatcga aatcactact gatgagtatc
   109621 tgcatgatat tcagcgcaaa atccaggcat taaccgaatc attgcctgtt gaagtattgc
   109681 tggtacgccg gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc
   109741 tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa gaactggatg
   109801 aatcgcagca gcaacgtctg cagcatcttt tcaccacgac gttgcatacc ctcgccggag
   109861 aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat
//
LOCUS       DAHBHC010000005       107943 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHBHC010000005 DAHBHC010000000
VERSION     DAHBHC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 107943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.005"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1063..1629
                     /locus_tag="M2A35_000443"
     CDS             1063..1629
                     /locus_tag="M2A35_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCB7196789.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTVDHGFHAHAVFWIDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESINNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1877..2452
                     /locus_tag="M2A35_000444"
     CDS             1877..2452
                     /locus_tag="M2A35_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196790.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPWEHDNAFNSRQTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
     gene            2521..2757
                     /locus_tag="M2A35_000445"
     CDS             2521..2757
                     /locus_tag="M2A35_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCB7196791.1"
                     /translation="MLTSMTGHDCVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2754..2912
                     /locus_tag="M2A35_000446"
     CDS             2754..2912
                     /locus_tag="M2A35_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196792.1"
                     /translation="MKRVAMSALVLRKSALSLVLATLPFIFLLTLSGQPADCSSFLTD
                     SSFHSHCL"
     gene            3000..3548
                     /locus_tag="M2A35_000447"
     CDS             3000..3548
                     /locus_tag="M2A35_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196793.1"
                     /translation="MQITEALISEPGDIRRFIQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEECSQVVDLLQQSWQVISAGGQCRVEKRFQVARGDTSGQYVALKTVALSL
                     GLPVVTAIIYRPVQRCTLITAQ"
     gene            complement(3567..3911)
                     /locus_tag="M2A35_000448"
     CDS             complement(3567..3911)
                     /locus_tag="M2A35_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HCB7196794.1"
                     /translation="MTKRQRKNRKRINRLVELWPELFNREKPQPLKVEIPDDLIQDIA
                     IRELAFGAGALRAAVASYVRSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     MLNDRRKARKYR"
     gene            complement(3908..4081)
                     /locus_tag="M2A35_000449"
     CDS             complement(3908..4081)
                     /locus_tag="M2A35_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCB7196795.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTAHNVKARERIPQLNDK
                     QSPALLKKHRGGI"
     gene            4544..5629
                     /gene="dndB"
                     /locus_tag="M2A35_000450"
     CDS             4544..5629
                     /gene="dndB"
                     /locus_tag="M2A35_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndB"
                     /protein_id="HCB7196796.1"
                     /translation="MASVDADYCYSFPAIRGIQAGRPFYIATCPMRIIPKIFSFDEND
                     VPPELRAQRTLNKSRIPEMVRYLLENPKDYVFSALTASIAVDVQFDEFPGTNNLGTLC
                     VPMDAQILINDGQHRRKAIEDALDARPELGQDNIPVLFFVDEGLKRSQQMFADLNKYA
                     IRPSPSLASLYDHRDASSNLARYLAMSVEPFVGMTELEKSNISQRSNKLFTLSSIKQA
                     TRALLGKDPKKSDFEECTQIATRYWQAIYNVMPDWQLAAKKEVSPVQLRQDYVHAHGI
                     GLQALGQLGYTLLTNYPEQWPDKIAALKVFNWRRNNPDLIKRAMQHGKLSKTSTAIQL
                     TCNALKNALSIPLTPEEQELEAQMVVS"
     gene            5626..7260
                     /gene="dndC"
                     /locus_tag="M2A35_000451"
     CDS             5626..7260
                     /gene="dndC"
                     /locus_tag="M2A35_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HCB7196797.1"
                     /translation="MSKLVQAYDLAEYTDFINQEQFSGRPLAEYISEVQRIYCADKRP
                     WVIGYSGGKDSSAVITLVYLALLGLPPEMRNKDVFVVSSDTLVETPVVVDLIKKTMLQ
                     IEAGAKRNGLPITQHAVTPKTNETFWVNLLGKGYPAPTRSFRWCTERMKINPVSDFIK
                     EKVSQFDEVIVVLGSRSSESASRAQVIAKHKIDGSRLARHTTLANAFIYTPIDTWDVE
                     DVWKLLRGAFRYAPEYIDEWESPWGGNNRSLWTLYMDSSAQGECPLVIDESTPSCGNS
                     RFGCWTCTVVTKDKAMESLIKNGEEWMSPLLKYRDLLAFTTDPVNKDTYRNYKRRTGK
                     VSYQYAKDGEDRSAERKHVPGPYWLKYRQQWLKDLLEIERDLNAQGHRITLITESELH
                     AIRQEWLKDPNEPDWYDTLPGIYREVYQRDLDWVVDDQSRFDASDADLLVQIAQGFDV
                     VPEMVMKLIELEVSMEGLSRRQGIFDKLGTILKQDWGSLEEIQQQQAALQKRNDRDIH
                     QEEITRLEAELQVLQRRIVDASESSVLVGEENERAH"
     gene            7250..9250
                     /gene="dndD"
                     /locus_tag="M2A35_000452"
     CDS             7250..9250
                     /gene="dndD"
                     /locus_tag="M2A35_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HCB7196798.1"
                     /translation="MLIKQLVLHNFRVFNGTHTIDLAPRKRPHEVNPRPIVLFGGLNG
                     AGKTSILSAIRLALYGRLAFGLATQQQDYIEHLSSLIHKGAYYIEQPEEAAVELTFTY
                     NKGGQESEFTVTRTWKKGKKDRLSLQKDGQPLSELDYDQCQGFLNELIPHGIADLFFF
                     DGEKIAELAEDESGNILRTAVRRLLGLDLISKLRNDLMIFVKRQQSSQLAETQQQQIE
                     VLEKQSRDLACQTEALLEKADFAKSRIEFLSKDIIRYEGLLNAQGGAFAQTKAQEKQK
                     VETLLKDKERLEKALRQECDGSLPYALAPNILSELLEKIADETQIKQAQSFEKELRLF
                     LAQLRNDIALRSRTTSKIAAEAITDNLNEYMATKPKGDLLFDISEREAGMLQQSIEQD
                     SKKAWQRFELYRIQLAEIEQQLEQAAANIARAPEDEQLMDLFEKLRDLDKQRENQRQK
                     YLSLLEDAKRVKQQQLDCVRQVQKLHDAARSQHGFSSAFKNAQETINLLDRYSDVLTQ
                     ARVKTLSANFEVAYRKLARKEDMQLSAHINPETFDVELIDEKGSVINRKLLSAGEKQI
                     YAIAILEALAKTSGRDLPVIIDTPLGRLDSQHRDKLINHYFPFASHQVVLLSTDTEVD
                     ERYFVDQLRDDISHAYEIVFNTHTKSSALKPGYFWELTKEAI"
     gene            9250..9606
                     /gene="dndE"
                     /locus_tag="M2A35_000453"
     CDS             9250..9606
                     /gene="dndE"
                     /locus_tag="M2A35_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndE"
                     /protein_id="HCB7196799.1"
                     /translation="MLPNRMVLNRQTEEQLKRLKGYTGITPNVAARLAFFRSVESEFR
                     YSSERDNRKLDGSLVLDKITWLGETLQTTELVLKMLYPQLAQKDMVKAWAAHVEDGIA
                     ALRNHKSLLNFIKSID"
     gene            complement(9665..10471)
                     /locus_tag="M2A35_000454"
     CDS             complement(9665..10471)
                     /locus_tag="M2A35_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HCB7196800.1"
                     /translation="MDGQHRLLGFKYAKNKDIQLPCSIYNSLPPSNQATIFSTINFNQ
                     QKVPKSLAYRLFGYSLDDISREYWPPDLLTVYFSEKFNKTDEYPFYRRLKNRVLNEIT
                     ENDWSISSSVFIDGVLSLISKNPRADRYTINAIDSDEKEKGRGRLDNKNSKDKSPLRW
                     FYIKGNDKAIEQILKIYFSAIKDHFWANVCIEKGTVLVRSVGISALFQFLRKKLMDMP
                     KINKENIEKLCSALKTVNPEEFTKNTEYTSTTVGQRKIYDYLNENVKTDF"
     gene            complement(10520..10825)
                     /locus_tag="M2A35_000455"
     CDS             complement(10520..10825)
                     /locus_tag="M2A35_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196801.1"
                     /translation="MSNSIEIHAIKVTQPYGEFFQTVMSAEDLLKCIYVNQAEMDDGE
                     ISGVQRKESMPRALDIAKFIDSDEAAFPNSIILSVNHNEDGTPAKEDDKWEFKKSEN"
     gene            complement(10826..11344)
                     /locus_tag="M2A35_000456"
     CDS             complement(10826..11344)
                     /locus_tag="M2A35_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCB7196802.1"
                     /translation="MSRNDMIHWVNLWKDILGLYPRIIQPKFIFISLVTLFVSTCGIW
                     IGPLFLKDNTQLGFSIFSFIITTLGVLAAECLSRDDENENPISLSERHRKQVRISCSI
                     FLWFISFVFLFFGLKNDVNFMLISSLIITIILWFSITVVKPDFKIPVTPDSLVSDELR
                     NMSSNDEPGEGL"
     gene            complement(11382..16436)
                     /gene="dptH"
                     /locus_tag="M2A35_000457"
     CDS             complement(11382..16436)
                     /gene="dptH"
                     /locus_tag="M2A35_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HCB7196803.1"
                     /translation="MSVKQYETYLAETFIEWVSGIIQPGERYQFKSPDPDNALQLWQA
                     FVDLAGDNHLEIAPEQRLACLSCNGIQLIPVLHGAAAPAFTENYISHLRDEVAGRSGV
                     FAKTALLIIHNSMLDTLINSTKDVAAPGAIWHPETFSHELEKLITTDNNRSELSRCLL
                     KDQRTTVLDEGATVFGFSSLYRLLDDGNLDFSELSLFKDDELLNFSQKQLHTRLNENR
                     KLFRQIEDSVERYSGQLENVLTEFSAKFIQEHFNDKDDWRELDFAVYLNEKEQNREQK
                     LVLDDITVENGVIWQRAKSASKAGKRDISLLVQVPPEQSQTELEFCFQGNDLQDNQIK
                     IAHHRQLKKERFWRISRAGGKSSRIMASVPFDGNPCFFSLELTNRNNSAEEYKFRLLL
                     VRQGQFWLDDIQHCFRIEPGKPQITLQREDNELRIAESGSQVCILDEENGDIDCQHYA
                     LVNFETLANQSDLIQFKLVSGDSCLAFNIEGPGAEEGLTLPLLFDQSRFNKLFKEDGN
                     ASWNRLKGRIILDNTEHKVVGVRQQLLALEASLVDQRLLGTGDDDSAFALDELVAIHP
                     DLYNAYDQLFLYYQRCGTLPSLVSWSAEYCALVSHIVTTFEQALQQIELSRALTAQEK
                     RLLHLGICNVDSHERLSPLHPLVLAYHLQLVQTICAEQEQYDSASFATLPTITLDRLV
                     VSGLMPFVYHSEHEYAQLQPVEENRFWIDVVPQRQVSHDYVKRLVKDKLNEFTEAYAR
                     LFQSPGNNALIINAINQGTAKELFLGLVEYFKQEKEHAISVHVNCYDERLLPNMFDRF
                     AESGSYEQLKNDLDLNRGAWRAEADMLIDLLRSRLTFSKFVLPSESDKLAYAHLAFFT
                     NTAPVDCRQIRIEDAASGVLCHGLISGEGAETQGDAYFTAFGLRNVDTESYCALRLAR
                     LVGCLWQPARQSNSQYHGQGIGLAVSGNFKQLLNYSYNSALWTTIIDPKVTLDFFTSQ
                     KDVVLIHYSDQYTSCAGYDAVTVTKQVDLFLRLLQTESQSGQSAVDSQHLLAEFNAFN
                     GEWLLKMLRSSEKERKEKYGIIGAYKFVQSMLSESDICWVPLSVAEMIRVSGNVGLKM
                     KESDLSRNLQGYRKGAISDDVLFVGFKENRLYLLPLEVKTGARPDYNYAGQQAAELKR
                     YLQQDILEPHTLASQLYRALFIRQVLMQVEKLQLYGVLDSDKLAPLLDRREWWLTGDY
                     QLGELKDYANGFVVAHVDSGSCFDLSYKETTANILQIEIPYSLLSSLITTREGKLPLA
                     ERYRVPDKYRLKPESDEHPSPSASGVQVTTPPDTRPDIPKPTPEVSTVPLQVLFGHDA
                     TRQTPLFWEPTNTTKFMNTNTGIIGTMGTGKTQFTKSLVTQLMRNQSCNVDGKSIGLL
                     IFDYKSDYVDEAFLKATGGKKYQLSLLPYNPLSLFGDMPMLPRHTAIAFSETMGKAYN
                     LGVKQRMKLVTLIMECYELAGIVPHDRSTWNRVAPTIEDVWQRYLAQEKVEEDSLYAA
                     LYNLAGFQIFETDPEKMTSLYDLVDGVTVIELAGYSSEIQNLVVALTLDLFYAQMQKR
                     GKPVIQGDYRQLTKMILVDEADNFMRQDFSSLRKILKEGREYGVGAILSTQEITHFKT
                     GENNYASYILTWVIHRVSEIKNADIKAVFNVDDKGEQESLMGQIRQLEKHFSLYVDGD
                     KRVSKMRDRAFWELVF"
     gene            complement(16417..17742)
                     /gene="dptG"
                     /locus_tag="M2A35_000458"
     CDS             complement(16417..17742)
                     /gene="dptG"
                     /locus_tag="M2A35_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HCB7196804.1"
                     /translation="MYPIATTLKVGNNQLDSYLPIRNKNNDINWQFVTGLVLSYALKR
                     KIDAYTPSQFRDDCKAHLQELLDEPAFWTVLERMYFSSEDIFRVSPLFLLFHAQFDGE
                     KISGASMADKRLGTLFANLMGDFSLEYPIQDKLNFIEQQMLNKLNEKIKLLGKGPFSE
                     EQPYLPYLVTCFQSDLAFLAEHPQYLLQELTNTLRLYAFSWCAQLALNLDNWQDGEPQ
                     SKSLFFILDTEKASSERDQIKRFGYKWFARQSEKLFPILSALEVLQIKGDKKRPLWQV
                     YQDCLNFSDNSSQLLQDLNNYLQEFIEKRELSASKYTQAVTLEEAFKQLLTVAVEQFQ
                     DKKSDRATVNRKYINELETQICTDFIQVRGRAGKVLVLNQDRLLLLTNLTVGKNDKLR
                     LHELLRGFEQRGFYLDNQSAQTLVAFYERMGNVERMSDSGDAVYVRKTV"
     gene            complement(17747..19396)
                     /gene="dptF"
                     /locus_tag="M2A35_000459"
     CDS             complement(17747..19396)
                     /gene="dptF"
                     /locus_tag="M2A35_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HCB7196805.1"
                     /translation="MSAITLRKALGVLAKSSSFSVTTVTHRQKDEFDQLKEQLFVKQE
                     IETELQRYLDVAKPGEIIFLCGSSGDGKSEILTRCKSNPRYQQRFSFHLDATHSFAPR
                     QSAIDALNDLFSNHHQYSSPLLIGINTGMLANFAREGAECHLAIRTAIDSFLSADQEE
                     SRPYRSGHCSFFDFEHYPKFQFNEKKQYSSFIKTLLDNLTRNDDSNLFQFIFRHDETV
                     NPELKEVANYKLLCLPGVQDVLITQLFKARLIKDQFVTTRTLLDFLHHLLMGPGYLFD
                     NLFTGAENDLIKKVSDFDPARLHTYEIDQFILRYELGLVDPELDDFLAALAPLHIRFD
                     RQCVNPGDAASLIRLFWLLQDESLGNNYHQKFSVFFNESLFEHYSEIWHLHKNYIADS
                     EQKKALNRFYTSELIAGIQRYANRKAPELSMQKEEFFLGEYGGVKITAPVELKPDWEA
                     IRNKNTAHPTGFDVHLKVGQNSLLPVRIGLNLFELLNKLNNGYRPNKYDKNAIVLLDE
                     IVDLITEQAKSSSEIKFYAGGRRVYRAKADDDMITISGMEG"
     gene            20009..20749
                     /locus_tag="M2A35_000460"
     CDS             20009..20749
                     /locus_tag="M2A35_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCB7196806.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDILLGVTYNF"
     gene            20923..22422
                     /locus_tag="M2A35_000461"
     CDS             20923..22422
                     /locus_tag="M2A35_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCB7196807.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLSQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSTAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNYYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARNFAGIFQ
                     WLTGIRTENITPFNPLTDEDNEPVMVFNGEKNVPADSLKPQPLILPDHR"
     gene            complement(22746..24011)
                     /locus_tag="M2A35_000462"
     CDS             complement(22746..24011)
                     /locus_tag="M2A35_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCB7196808.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWATELSLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFCGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKFISPFEYAKINNPLKQ"
     gene            complement(24198..24282)
                     /locus_tag="M2A35_000463"
     tRNA            complement(24198..24282)
                     /locus_tag="M2A35_000463"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24246..24248),aa:Leu,seq:caa)
     gene            24478..25497
                     /gene="ahr"
                     /locus_tag="M2A35_000464"
     CDS             24478..25497
                     /gene="ahr"
                     /locus_tag="M2A35_000464"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCB7196809.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            25627..27129
                     /locus_tag="M2A35_000465"
     CDS             25627..27129
                     /locus_tag="M2A35_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCB7196810.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQKNPPTKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(27248..28330)
                     /gene="lptG"
                     /locus_tag="M2A35_000466"
     CDS             complement(27248..28330)
                     /gene="lptG"
                     /locus_tag="M2A35_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCB7196811.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(28330..29430)
                     /gene="lptF"
                     /locus_tag="M2A35_000467"
     CDS             complement(28330..29430)
                     /gene="lptF"
                     /locus_tag="M2A35_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCB7196812.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            29697..31208
                     /gene="pepA"
                     /locus_tag="M2A35_000468"
     CDS             29697..31208
                     /gene="pepA"
                     /locus_tag="M2A35_000468"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCB7196813.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            31342..31785
                     /gene="holC"
                     /locus_tag="M2A35_000469"
     CDS             31342..31785
                     /gene="holC"
                     /locus_tag="M2A35_000469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCB7196814.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            31785..34640
                     /gene="valS"
                     /locus_tag="M2A35_000470"
     CDS             31785..34640
                     /gene="valS"
                     /locus_tag="M2A35_000470"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCB7196815.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(34696..35892)
                     /locus_tag="M2A35_000471"
     CDS             complement(34696..35892)
                     /locus_tag="M2A35_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCB7196816.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            36085..36588
                     /locus_tag="M2A35_000472"
     CDS             36085..36588
                     /locus_tag="M2A35_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7196817.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSQSDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(36634..37050)
                     /gene="rraB"
                     /locus_tag="M2A35_000473"
     CDS             complement(36634..37050)
                     /gene="rraB"
                     /locus_tag="M2A35_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCB7196818.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            37212..38216
                     /gene="argF"
                     /locus_tag="M2A35_000474"
     CDS             37212..38216
                     /gene="argF"
                     /locus_tag="M2A35_000474"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCB7196819.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKKEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(38317..38547)
                     /locus_tag="M2A35_000475"
     CDS             complement(38317..38547)
                     /locus_tag="M2A35_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196820.1"
                     /translation="MKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKTNEIVKN
                     NPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
     gene            complement(38534..39877)
                     /locus_tag="M2A35_000476"
     CDS             complement(38534..39877)
                     /locus_tag="M2A35_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCB7196821.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIDSLDDAKVNLTPVIDSILEIFLKFPYINDVRILDWCFNKRMQYF
                     GDSEKIKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEHYKNSRKLQELI
                     WDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFFEGFFHEAT"
     gene            complement(40000..40452)
                     /gene="tabA"
                     /locus_tag="M2A35_000477"
     CDS             complement(40000..40452)
                     /gene="tabA"
                     /locus_tag="M2A35_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCB7196822.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(40597..41190)
                     /locus_tag="M2A35_000478"
     CDS             complement(40597..41190)
                     /locus_tag="M2A35_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCB7196823.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKV"
     gene            41261..41974
                     /gene="bdcA"
                     /locus_tag="M2A35_000479"
     CDS             41261..41974
                     /gene="bdcA"
                     /locus_tag="M2A35_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7196824.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            42105..42500
                     /locus_tag="M2A35_000480"
     CDS             42105..42500
                     /locus_tag="M2A35_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCB7196825.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            42781..42915
                     /gene="pyrL"
                     /locus_tag="M2A35_000481"
     CDS             42781..42915
                     /gene="pyrL"
                     /locus_tag="M2A35_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCB7196826.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            42919..43854
                     /gene="pyrB"
                     /locus_tag="M2A35_000482"
     CDS             42919..43854
                     /gene="pyrB"
                     /locus_tag="M2A35_000482"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCB7196827.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            43867..44328
                     /gene="pyrI"
                     /locus_tag="M2A35_000483"
     CDS             43867..44328
                     /gene="pyrI"
                     /locus_tag="M2A35_000483"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCB7196828.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            44401..44787
                     /gene="ridA"
                     /locus_tag="M2A35_000484"
     CDS             44401..44787
                     /gene="ridA"
                     /locus_tag="M2A35_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCB7196829.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            44853..45308
                     /locus_tag="M2A35_000485"
     CDS             44853..45308
                     /locus_tag="M2A35_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7196830.1"
                     /translation="MAKPRFTNEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFICFFIFAVIYFFIQVFS"
     gene            complement(45353..48049)
                     /gene="mgtA"
                     /locus_tag="M2A35_000486"
     CDS             complement(45353..48049)
                     /gene="mgtA"
                     /locus_tag="M2A35_000486"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCB7196831.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(48190..48243)
                     /gene="mgtL"
                     /locus_tag="M2A35_000487"
     CDS             complement(48190..48243)
                     /gene="mgtL"
                     /locus_tag="M2A35_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCB7196832.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            48428..49375
                     /gene="treR"
                     /locus_tag="M2A35_000488"
     CDS             48428..49375
                     /gene="treR"
                     /locus_tag="M2A35_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCB7196833.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            49494..50915
                     /gene="treB"
                     /locus_tag="M2A35_000489"
     CDS             49494..50915
                     /gene="treB"
                     /locus_tag="M2A35_000489"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCB7196834.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            50965..52620
                     /gene="treC"
                     /locus_tag="M2A35_000490"
     CDS             50965..52620
                     /gene="treC"
                     /locus_tag="M2A35_000490"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCB7196835.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPTSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            53014..55152
                     /gene="nrdD"
                     /locus_tag="M2A35_000491"
     CDS             53014..55152
                     /gene="nrdD"
                     /locus_tag="M2A35_000491"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCB7196836.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            55310..55774
                     /gene="nrdG"
                     /locus_tag="M2A35_000492"
     CDS             55310..55774
                     /gene="nrdG"
                     /locus_tag="M2A35_000492"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCB7196837.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QLFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(55819..56205)
                     /gene="cybC"
                     /locus_tag="M2A35_000493"
     CDS             complement(55819..56205)
                     /gene="cybC"
                     /locus_tag="M2A35_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCB7196838.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(56388..57740)
                     /gene="pmbA"
                     /locus_tag="M2A35_000494"
     CDS             complement(56388..57740)
                     /gene="pmbA"
                     /locus_tag="M2A35_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCB7196839.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            57834..58385
                     /locus_tag="M2A35_000495"
     CDS             57834..58385
                     /locus_tag="M2A35_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCB7196840.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(58541..59914)
                     /gene="mpl"
                     /locus_tag="M2A35_000496"
     CDS             complement(58541..59914)
                     /gene="mpl"
                     /locus_tag="M2A35_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCB7196841.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            60090..61088
                     /gene="fbp"
                     /locus_tag="M2A35_000497"
     CDS             60090..61088
                     /gene="fbp"
                     /locus_tag="M2A35_000497"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCB7196842.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(61121..62116)
                     /gene="yjfF"
                     /locus_tag="M2A35_000498"
     CDS             complement(61121..62116)
                     /gene="yjfF"
                     /locus_tag="M2A35_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCB7196843.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(62103..63125)
                     /locus_tag="M2A35_000499"
     CDS             complement(62103..63125)
                     /locus_tag="M2A35_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7196844.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(63139..64641)
                     /locus_tag="M2A35_000500"
     CDS             complement(63139..64641)
                     /locus_tag="M2A35_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCB7196845.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(64781..65737)
                     /gene="ytfQ"
                     /locus_tag="M2A35_000501"
     CDS             complement(64781..65737)
                     /gene="ytfQ"
                     /locus_tag="M2A35_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCB7196846.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            66047..66577
                     /gene="ppa"
                     /locus_tag="M2A35_000502"
     CDS             66047..66577
                     /gene="ppa"
                     /locus_tag="M2A35_000502"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCB7196847.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(66657..67007)
                     /gene="chpB"
                     /locus_tag="M2A35_000503"
     CDS             complement(66657..67007)
                     /gene="chpB"
                     /locus_tag="M2A35_000503"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HCB7196848.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(67001..67252)
                     /gene="chpS"
                     /locus_tag="M2A35_000504"
     CDS             complement(67001..67252)
                     /gene="chpS"
                     /locus_tag="M2A35_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HCB7196849.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(67465..67806)
                     /locus_tag="M2A35_000505"
     CDS             complement(67465..67806)
                     /locus_tag="M2A35_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCB7196850.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(67809..71588)
                     /gene="tamB"
                     /locus_tag="M2A35_000506"
     CDS             complement(67809..71588)
                     /gene="tamB"
                     /locus_tag="M2A35_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCB7196851.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNCTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(71585..73318)
                     /gene="tamA"
                     /locus_tag="M2A35_000507"
     CDS             complement(71585..73318)
                     /gene="tamA"
                     /locus_tag="M2A35_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCB7196852.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            73524..74162
                     /gene="msrA"
                     /locus_tag="M2A35_000508"
     CDS             73524..74162
                     /gene="msrA"
                     /locus_tag="M2A35_000508"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCB7196853.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            74485..75828
                     /locus_tag="M2A35_000509"
     CDS             74485..75828
                     /locus_tag="M2A35_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCB7196854.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(75890..76096)
                     /locus_tag="M2A35_000510"
     CDS             complement(75890..76096)
                     /locus_tag="M2A35_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCB7196855.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            76421..76978
                     /locus_tag="M2A35_000511"
     CDS             76421..76978
                     /locus_tag="M2A35_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCB7196856.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(76968..77708)
                     /gene="cysQ"
                     /locus_tag="M2A35_000512"
     CDS             complement(76968..77708)
                     /gene="cysQ"
                     /locus_tag="M2A35_000512"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCB7196857.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            77898..79841
                     /gene="cpdB"
                     /locus_tag="M2A35_000513"
     CDS             77898..79841
                     /gene="cpdB"
                     /locus_tag="M2A35_000513"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCB7196858.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(79970..80350)
                     /locus_tag="M2A35_000514"
     CDS             complement(79970..80350)
                     /locus_tag="M2A35_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCB7196859.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            80439..81299
                     /gene="qorB"
                     /locus_tag="M2A35_000515"
     CDS             80439..81299
                     /gene="qorB"
                     /locus_tag="M2A35_000515"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCB7196860.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            81408..82373
                     /locus_tag="M2A35_000516"
     CDS             81408..82373
                     /locus_tag="M2A35_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCB7196861.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            82481..83143
                     /gene="ytfE"
                     /locus_tag="M2A35_000517"
     CDS             82481..83143
                     /gene="ytfE"
                     /locus_tag="M2A35_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCB7196862.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(83188..84600)
                     /gene="cycA"
                     /locus_tag="M2A35_000518"
                     /gene_synonym="dagA"
     CDS             complement(83188..84600)
                     /gene="cycA"
                     /locus_tag="M2A35_000518"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCB7196863.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMVFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(84909..85529)
                     /gene="fklB"
                     /locus_tag="M2A35_000519"
     CDS             complement(84909..85529)
                     /gene="fklB"
                     /locus_tag="M2A35_000519"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCB7196864.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            85748..86386
                     /gene="ytfB"
                     /locus_tag="M2A35_000520"
     CDS             85748..86386
                     /gene="ytfB"
                     /locus_tag="M2A35_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCB7196865.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(86370..87017)
                     /locus_tag="M2A35_000521"
     CDS             complement(86370..87017)
                     /locus_tag="M2A35_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCB7196866.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            87230..88021
                     /locus_tag="M2A35_000522"
     CDS             87230..88021
                     /locus_tag="M2A35_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCB7196867.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            88031..88873
                     /locus_tag="M2A35_000523"
     CDS             88031..88873
                     /locus_tag="M2A35_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCB7196868.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            88883..89659
                     /locus_tag="M2A35_000524"
     CDS             88883..89659
                     /locus_tag="M2A35_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCB7196869.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            89669..91210
                     /locus_tag="M2A35_000525"
     CDS             89669..91210
                     /locus_tag="M2A35_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCB7196870.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            91207..93288
                     /locus_tag="M2A35_000526"
     CDS             91207..93288
                     /locus_tag="M2A35_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCB7196871.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            93309..93785
                     /locus_tag="M2A35_000527"
     CDS             93309..93785
                     /locus_tag="M2A35_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCB7196872.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            93828..95129
                     /locus_tag="M2A35_000528"
     CDS             93828..95129
                     /locus_tag="M2A35_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCB7196873.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            95182..96153
                     /locus_tag="M2A35_000529"
     CDS             95182..96153
                     /locus_tag="M2A35_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCB7196874.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(96265..96714)
                     /gene="rplI"
                     /locus_tag="M2A35_000530"
     CDS             complement(96265..96714)
                     /gene="rplI"
                     /locus_tag="M2A35_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCB7196875.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(96756..96983)
                     /gene="rpsR"
                     /locus_tag="M2A35_000531"
     CDS             complement(96756..96983)
                     /gene="rpsR"
                     /locus_tag="M2A35_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCB7196876.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(96988..97302)
                     /gene="priB"
                     /locus_tag="M2A35_000532"
     CDS             complement(96988..97302)
                     /gene="priB"
                     /locus_tag="M2A35_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCB7196877.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(97309..97704)
                     /gene="rpsF"
                     /locus_tag="M2A35_000533"
     CDS             complement(97309..97704)
                     /gene="rpsF"
                     /locus_tag="M2A35_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCB7196878.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            98031..98306
                     /locus_tag="M2A35_000534"
     CDS             98031..98306
                     /locus_tag="M2A35_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7196879.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(98435..99121)
                     /gene="ulaF"
                     /locus_tag="M2A35_000535"
     CDS             complement(98435..99121)
                     /gene="ulaF"
                     /locus_tag="M2A35_000535"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCB7196880.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(99121..99975)
                     /gene="ulaE"
                     /locus_tag="M2A35_000536"
     CDS             complement(99121..99975)
                     /gene="ulaE"
                     /locus_tag="M2A35_000536"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCB7196881.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(99985..100635)
                     /gene="ulaD"
                     /locus_tag="M2A35_000537"
     CDS             complement(99985..100635)
                     /gene="ulaD"
                     /locus_tag="M2A35_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCB7196882.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPMFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(100649..101113)
                     /gene="ulaC"
                     /locus_tag="M2A35_000538"
     CDS             complement(100649..101113)
                     /gene="ulaC"
                     /locus_tag="M2A35_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCB7196883.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(101123..101428)
                     /gene="ulaB"
                     /locus_tag="M2A35_000539"
     CDS             complement(101123..101428)
                     /gene="ulaB"
                     /locus_tag="M2A35_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCB7196884.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSKIDHTVNSCAVGEY
                     KSELSNADIIIASTHIAGEISVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(101444..102841)
                     /gene="ulaA"
                     /locus_tag="M2A35_000540"
     CDS             complement(101444..102841)
                     /gene="ulaA"
                     /locus_tag="M2A35_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCB7196885.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYN
                     WVGYAVLLALALNICYVLLHRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            103196..104260
                     /gene="ulaG"
                     /locus_tag="M2A35_000541"
     CDS             103196..104260
                     /gene="ulaG"
                     /locus_tag="M2A35_000541"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCB7196886.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            104368..105123
                     /gene="ulaR"
                     /locus_tag="M2A35_000542"
     CDS             104368..105123
                     /gene="ulaR"
                     /locus_tag="M2A35_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCB7196887.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(105120..105869)
                     /gene="yjfP"
                     /locus_tag="M2A35_000543"
     CDS             complement(105120..105869)
                     /gene="yjfP"
                     /locus_tag="M2A35_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCB7196888.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            106051..106380
                     /gene="bsmA"
                     /locus_tag="M2A35_000544"
     CDS             106051..106380
                     /gene="bsmA"
                     /locus_tag="M2A35_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCB7196889.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            106529..106804
                     /gene="yjfN"
                     /locus_tag="M2A35_000545"
     CDS             106529..106804
                     /gene="yjfN"
                     /locus_tag="M2A35_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCB7196890.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(106921..>107943)
                     /locus_tag="M2A35_000546"
     CDS             complement(106921..>107943)
                     /locus_tag="M2A35_000546"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCB7196891.1"
                     /translation="RLEDGSYRLVGHKWFFSVPQSDAHLVLAQTTGGLSCFFVPRFLP
                     DGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGQEGEGIRLILKMGGMTRFDCA
                     LGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLARA
                     WDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMP
                     VNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKP
                     AEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLLLRA
                     TGGVCV"
BASE COUNT        27405 a        27270 c        26350 g        26918 t
ORIGIN      
        1 atcatgtaaa agtcctctct gctcagcatg ttgatgtcat ttctggtgtg agaaccttaa
       61 ggaaacaaca tgttgggtgg agcggaaaat ccaaatggtg accgtcttat tatcgctggc
      121 gctgacacct gagcgatcac ctttaatatg gtagcaatta cactgaatta cttacagatt
      181 cgttataacg aacctctgta cataattatg aaacagattt gtatttacat aaaacatgga
      241 tatttttaat ttaggtttat tactattatt atactcactc cctttttgat ataatctcta
      301 ttgttattta cttcctgtct ttactcactc tctgttcctg ttttcagtga aataataatt
      361 tattttaatt ttatccatca tgagctgtat ttgcttaaca atgattgttt gcctgatatg
      421 ttttaactat tctggtgaac atcaattctc tgtcatcact gattaactga atgcttacgc
      481 tttgataagg tatccatgat tccgtcatgt ttaacagcgc agaataaaca atgaaattaa
      541 cagagtgaat tcctgattat gtttgtttcg ggttgtattg tttaagatgc agagtaaaaa
      601 tcatgccagt ggtatttttt actctggtaa tgtgtgcggt taacgttcat gtccttaaga
      661 acagattgga tgagggcgta taagtttatt gcgttggcct tgttcacgtt tttttagaac
      721 cactgagagc aagtttgtag tttatgattg attggtcgaa atatatttct taaccttctg
      781 gttgtgggtt tttatcgcgt gttttgcagt atttcgtgat gtttattgat tctgtatttt
      841 attcgctcct cgtttatctc atctctttag caaataccat cagataatcc atttctttct
      901 gcataatgct gcgtatcgtt aataacccgt cgtattcatt ctgctacagc atgcctgata
      961 aataccatct gtaagttatt accgttttag atctgattat gagcgaaagc attaattcgc
     1021 tcacagagct taaaacatca ttaactttca ggagtcatca acatgcctaa atcttataca
     1081 ccaaactggt tttttaccgc tttacttgac aatcacatca atcaaatgat ggcacgctat
     1141 tcctgcctgc gggccttacg catggatttc ttctacagga aagatacgcc tgatttctta
     1201 caacctgatc atcgctggct tgaattgcag ttgcgtatgc tgctggagca ggtagaacaa
     1261 tttgaaaata tcgttggctt cttctgggtg attgaatgga cggttgatca tggttttcat
     1321 gcgcatgcgg ttttctggat cgatcgtcag agggttaaaa aaatatatcc ctttgcggag
     1381 cggattacgg aatgctggcg gtctattacg cataacagcg gttcggcaca ccgctgtaca
     1441 tatcagccgc attatacata caacatcaac attcctgtgc gccacaacga tcctgaaagc
     1501 atcaataata ttcgcggtgc cctgcattat ctggcgaaag aagagcaaaa agacgggctg
     1561 tgtgcctacg gctgcaatga agttcctgaa cgtcctgcag cagggcgtcc tcgtaagcct
     1621 cacttctgaa agtttgaatc atgagtttcc tggcccggaa acacttagtc ggtaaaaact
     1681 tacctcgatg attaatgatg tgaactgaag tcaacggaga tcattcaccc tgaacctgca
     1741 tccggtgttc tgtcccttat cttcccgttc tgcttcagtt cttcacttat tccatcaatc
     1801 tcatccgcaa gccataacac gtcagctcat tcacgggcag ggcgcgttgt gggttgcgca
     1861 taatggagca tatcttatga atgctatccc ttatttcgac tatagcctgg cacccttctg
     1921 gccatcttat cagaacaaag tcatcggcgt ccttgagcgt gcgctgcgtg agcagtccgg
     1981 ctcacggata cggcggatcc tgcttcgtct gccgtgggaa catgacaacg ccttcaacag
     2041 cagacagacc tggtttggta tggattttat cgaaaccgtc agtgcgctga tgaatgcgaa
     2101 acccggacgc gacctttgct ggctcctgac ccgtcatccg gaaaagccgg aataccacgt
     2161 ggtgctgtgc gtcagacagg agtatttcga cggccccgaa ctggaccggc tgattctgga
     2221 tgcctggagc aacgtactgg gcttcgcgtc accgggggaa gcggcaccgt accagaaaca
     2281 gataacccgg gatgtggtgc tggacagtcg ttcaccggac tgcgaagaca tcctcaaaga
     2341 tttgatctgg gcgttcagtg atttcgcccg cgatcgtcgt ggtgtgtgcg atccggaagc
     2401 ccgttgcctc gccggtaatc ccggctatcc cggatctgca ggaccgttct gagaatacgg
     2461 ctcagcacct ttccccgcat tcgcacaacc ttcataaatc tcacagagga tattctgacc
     2521 atgttgacct caatgacagg ccacgactgc gtgttgctgc gtgccgacga tcccctgatc
     2581 gacatgaact acatcaccag tttcaccggt atgacagata aatggtttta caagctgatc
     2641 agtgaaggcc atttcccgaa acccatcaag ctgggccgca gcagccgctg gtacaaaagt
     2701 gaagtggagc agtggatgca gcagcgaatt gaggaatcac gaggagcagc agcatgaaac
     2761 gtgttgcaat gtcggcgctg gtattgcgga aatcagccct gtccctggtg ctggcgacat
     2821 taccgttcat cttcctgctg accctctccg gtcagccagc agattgtagc tctttcctga
     2881 ctgattcgtc attccattca cattgtttat aactggcatt acaccggtgt tgatgcgcac
     2941 cttcccgtgt gtctgcaccg gctgaacaaa attcaacagg gtttgaaaag gaacatttcg
     3001 tgcaaataac cgaagcatta atttcagagc cgggagacat ccggcgtttt attcagcagg
     3061 cggtggacca ctggccgcat ttgctggcat tttacttcac actccattcg gcagaaggaa
     3121 atatcaacgg gcaacagatt catgcattct gtacttcctt ttaccgacag gtgcacgaac
     3181 gtattactga gcgtaatcac acggccagtc catcctcgcc ggtggtatta cgctggttgc
     3241 gggaacaaca tggaggagca acaatacgat gcctgttgct gttcagccag gagcttttct
     3301 gccatccgcg agccagtgtg acagttgatg aagaatgttc gcaagtggtg gatttactgc
     3361 aacagagctg gcaggtgata agtgctggcg gacaatgccg ggtggaaaag cgttttcagg
     3421 tcgcccgggg tgatacatct ggccagtatg ttgcgttaaa aacagtcgca ttgtctctgg
     3481 ggttaccggt tgtgaccgcc attatctatc gtccggtaca gcgctgtaca ttgattacag
     3541 ctcagtgaat cagcgcagtt gtgcagtcaa cgatattttc tggcttttcg tcggtcattc
     3601 agcatcatca gccgggtctc tgcctctttc tgttcctgtg gagtcacttc cccgcacggc
     3661 tgccccttca ggtcataacg ggcgccgcca gccattaacg cacggtaata gcgcggagac
     3721 cgcacataag acgctactgc cgcgcgtaat gcacctgcgc caaacgccag ttctctgatg
     3781 gcgatatcct gtatcaggtc gtcaggtatc tcaactttaa gcggttgggg tttttcccgg
     3841 ttgaataact caggccagag ttccacaaga cgattaatac gcttacggtt tttacgctgg
     3901 cgttttgtca tatgccgcca cggtgttttt tcagcagagc agggctctgt ttatcgttca
     3961 actgtggtat ccgttccctg gccttcacgt tgtgtgcagt gttcgtttta tcctgcagcg
     4021 gcgttgccgg tgacttactg aaaataccct gcggtttacg gttaatggtc agcgttacca
     4081 tcagtattgt tacctcgtgc tgtcaatgga agtggtacct catccggtat ctgaaaatgt
     4141 tttccggtgg atggcacagc gaatgtcatg taagacatgc tgttgccggt attgttcagt
     4201 gctgtgcgta gtgagcgtat tcgttatcag actgtactgg ttgtgaacga tatgactatg
     4261 gtgaccgcta cagtcggacc agccgtacca atacatacag ccatgtgccc tctgatgctg
     4321 aaagcctaaa tgatggtaat gacgacatgc gtatgataaa atattacttg acaaatgata
     4381 atgaacacca cttgcaacaa ccagcaagac cattgaatag agctaattgg cgagtacatc
     4441 tgtagatagt acacaattca ttacgtaaaa tgctgtaatt tctatctagg gttacgtaaa
     4501 atatcacgta acttaattca ttaaatattt tacggattac agcatggcta gtgttgatgc
     4561 agactactgc tactcgtttc cggctatacg tggcattcag gcaggccgcc ccttctacat
     4621 tgccacatgt ccgatgcgta tcatccccaa aatttttagc tttgatgaaa atgatgttcc
     4681 tccggagttg cgcgcgcaac gcaccctcaa taaatctcgc atccctgaaa tggttcgtta
     4741 tcttcttgaa aatcctaaag attatgtatt ttctgcacta acagcatcca ttgccgttga
     4801 cgtccagttt gatgagtttc ccggcacgaa taatctaggg acgttatgtg taccaatgga
     4861 cgcgcaaatc ttgatcaacg acggtcagca tcgtcgtaaa gcgattgaag acgctttaga
     4921 cgctcgcccg gagctggggc aggataacat acctgtgctg ttttttgtcg atgaaggatt
     4981 aaaacgcagc caacagatgt ttgccgatct gaataaatat gctattcgcc ccagtccgtc
     5041 gttggcatcg ttgtatgatc atcgggatgc cagttcaaat ttggcgcgtt atctggcaat
     5101 gtccgtagag ccgtttgtcg gaatgactga gctggaaaaa tccaatatca gtcagcggtc
     5161 aaataaatta tttacgttaa gcagcatcaa gcaagctacc cgtgcgttgc tgggaaaaga
     5221 tccgaaaaag agcgattttg aagaatgtac ccagatagca actcgctatt ggcaagctat
     5281 ttataatgta atgccggact ggcaattagc cgcaaaaaag gaagtttcac cggttcaact
     5341 acgtcaggac tatgttcacg ctcacggtat tggtttgcag gcactcggac aactagggta
     5401 tacactgctg acaaattacc cagagcagtg gcctgataaa attgctgcac tcaaagtgtt
     5461 taactggcgc agaaataatc cggatcttat caaacgtgcg atgcagcatg gaaagttgag
     5521 caaaaccagt acggcaatcc aactcacctg caatgcgcta aaaaacgcac tctcaattcc
     5581 gcttactcct gaagaacagg agcttgaagc ccaaatggtt gtctcatgag taaattagtt
     5641 caggcctacg atttggccga atacacagat tttattaatc aggagcagtt ttctggacgc
     5701 cctctggccg aatatatttc tgaagttcag agaatttatt gtgcggataa gcgtccgtgg
     5761 gtaattggct atagcggtgg aaaagattct tccgccgtga tcacgctggt gtatctggcc
     5821 ctgcttggcc tgcccccgga aatgcgcaac aaagatgttt ttgtagtgtc atccgatacg
     5881 ctggtggaaa caccagtggt agtagattta attaagaaaa cgatgttgca aattgaggca
     5941 ggcgcaaagc gtaatggcct gccgatcact caacatgcgg taacgcctaa aactaatgaa
     6001 accttctggg tcaatttact ggggaaaggt tatccggccc caacccgcag ttttcgctgg
     6061 tgtaccgaac gcatgaaaat caatccggtg agtgacttta ttaaagaaaa ggtcagccag
     6121 tttgatgaag taattgttgt cctaggttca cgtagtagtg agagtgcctc tcgtgcgcag
     6181 gtcatcgcaa agcacaaaat tgatggctca cgcttggccc ggcataccac actggcaaat
     6241 gcgtttatct atacccctat tgatacctgg gatgtagaag acgtctggaa actgctgcgc
     6301 ggcgcgttcc gctatgctcc tgaatatatc gacgaatggg aaagcccatg gggtggaaac
     6361 aatcgttctc tatggacgct gtatatggac tcttccgcac agggtgagtg tcccttagtg
     6421 atagacgaaa gtaccccctc ttgtgggaac tctcgctttg gatgctggac ctgtaccgta
     6481 gtaaccaaag ataaagcgat ggaaagtctg atcaaaaatg gcgaagagtg gatgtcgcca
     6541 ttgctgaaat atcgtgatct gctggcgttt accaccgatc cggtgaataa agacacctat
     6601 cgcaactata aacgtcgtac cggtaaggtg agctatcagt acgctaaaga cggtgaagat
     6661 cgcagtgcag agcgtaagca tgtgccaggc ccctactggc tcaaatatcg ccagcaatgg
     6721 ttaaaagact tactggaaat tgagcgtgat ttaaacgccc agggccacag gattacgtta
     6781 atcaccgagt cggaactgca cgctatccgc caagaatggt tgaaggatcc taatgaacca
     6841 gactggtacg acacactacc aggtatctac cgtgaggtgt accagcggga tctggattgg
     6901 gtcgttgacg accaatcacg ttttgacgcc agcgatgccg atttgctggt gcagatagct
     6961 caggggtttg atgttgtgcc agagatggtg atgaaattga ttgagctgga agtttcaatg
     7021 gaaggattaa gtcgccgtca ggggattttt gacaaacttg gcaccatttt aaaacaagat
     7081 tggggcagtc tcgaagagat tcaacaacag caggcagcgc tacaaaaacg taacgaccgg
     7141 gatattcacc aggaagaaat caccaggctg gaagctgaac ttcaggtttt acaacgcaga
     7201 attgtcgatg ccagcgaatc ctctgtattg gtcggcgagg aaaacgaacg tgctcattaa
     7261 acaactggta ttacataatt ttcgggtatt taacggtact cacacgattg atctggctcc
     7321 aagaaagcga ccacatgaag taaaccctcg tccgattgtg ctgtttggcg gtttaaatgg
     7381 tgcaggtaaa acgtctattc tgtcggctat ccggctggca ttgtatgggc ggctggcatt
     7441 tgggctcgcg actcagcaac aggattatat tgagcatctt agttcgttga ttcacaaagg
     7501 tgcgtactat attgagcaac cggaagaggc ggctgttgaa ctgaccttta cctataataa
     7561 gggtgggcag gaatcagagt ttactgttac gcgaacttgg aaaaagggca agaaagatcg
     7621 cctttcttta caaaaggatg gccaaccact cagtgaactg gattatgatc agtgccaggg
     7681 gtttcttaat gaattaattc ctcatggtat tgctgatttg ttcttttttg atggcgaaaa
     7741 aattgcggaa ctggcagaag atgaatctgg caatattttg cgcaccgctg tacgccgttt
     7801 attgggcctg gatctgatat ccaaactgcg taatgactta atgattttcg ttaagcgcca
     7861 acaatccagc cagttagcgg aaactcagca acagcagatt gaagtgctgg aaaaacaaag
     7921 tagagactta gcttgtcaga cagaagcatt gctggaaaaa gctgattttg ccaaatctag
     7981 aatcgagttt ctgtctaaag acattattcg ctatgaaggc ttgttgaatg cgcagggcgg
     8041 tgcatttgcg cagacaaaag cgcaggagaa gcagaaagtt gaaacactgc taaaagataa
     8101 ggagcgcctg gaaaaagcac tgcgtcagga atgtgatgga tcattacctt atgcactagc
     8161 tcccaatatt ttatccgagt tgctggaaaa aattgctgat gaaacgcaga tcaaacaagc
     8221 tcaaagcttt gaaaaagagt taaggctatt tttggcccag ctcagaaacg atattgcatt
     8281 acgctctcgt actaccagta aaattgcagc cgaggcgatt actgataatc tgaacgaata
     8341 tatggcaact aagccgaagg gcgatttgtt gtttgatatt tctgaacgtg aagccggcat
     8401 gctccagcaa tcaatagagc aggacagtaa aaaggcatgg cagcgcttcg agctttaccg
     8461 tattcagtta gcagaaattg aacagcaact agagcaagcc gctgccaata ttgcccgcgc
     8521 tccggaagat gagcagttaa tggatctttt tgaaaagctg cgcgatcttg ataagcagcg
     8581 tgaaaatcag cggcaaaaat atctttcatt gctggaagac gctaaacggg ttaagcaaca
     8641 gcaactggat tgtgtccgtc aggtgcaaaa actgcatgat gcggcccgca gtcagcatgg
     8701 ctttagtagc gccttcaaaa atgcgcagga aacaattaat ttactcgatc ggtatagcga
     8761 cgtattaact caggcgagag taaagacgct atctgctaac tttgaagttg cctatcgcaa
     8821 actggctcgt aaagaagata tgcaactgag tgctcatatt aacccagaga catttgacgt
     8881 tgagttaatt gatgagaagg gatctgtcat taatcgtaag ttactatctg ccggtgaaaa
     8941 gcagatttat gccattgcta ttcttgaggc gctagcaaaa acttctggtc gtgatttgcc
     9001 ggtgattatt gatacgccat taggacgtct ggattcacaa catcgggata aactaattaa
     9061 ccattatttc ccatttgcca gccatcaagt ggtgctattg tctaccgata cggaagtgga
     9121 tgaacgttat tttgttgacc aattacgtga tgatatttct catgcttacg agatagtatt
     9181 taacacgcat actaaatcat cagcgttgaa gcccggttat ttctgggaat taactaagga
     9241 ggctatctga tgctcccgaa tcgaatggtg ctgaatcgtc agactgaaga gcagcttaaa
     9301 agattgaaag ggtatacagg gatcacacct aatgtcgctg cccgattggc atttttccgc
     9361 tcggtggaga gtgagtttcg ctattcatca gaacgggata atcggaaact agatggttcg
     9421 ctggtgttgg ataagattac gtggctgggg gagacattac agactacgga attggtattg
     9481 aagatgcttt atccgcagtt agctcagaag gatatggtta aagcatgggc tgcgcatgtg
     9541 gaggatggga ttgcggcgtt aaggaatcat aagagtctat taaactttat taaatcaata
     9601 gattaagaat ttttaggcca tccaaattaa caatatggat ggcctgaaca aaatttagtc
     9661 aattttaaaa atctgttttg acattttcgt ttaaataatc atatattttt cgctgaccca
     9721 cagttgttga tgtatactct gtattttttg taaactcctc aggattcaca gttttcaatg
     9781 cagagcacag cttttcaata ttttctttgt taattttagg catgtccatc aattttttac
     9841 gtagaaactg aaataatgca gaaataccga cagatctaac taacactgtg cctttctcaa
     9901 tacatacatt agcccaaaaa tgatctttta tcgctgaaaa ataaattttc aatatttgct
     9961 ctatcgcctt atcattgcct tttatataaa accatcgcaa tgggctttta tccttgctgt
    10021 ttttattatc aagcctgccc cttccttttt ctttttcatc cgaatcaatt gcattgatgg
    10081 tatatctatc agcacgaggg tttttagata ttagtgataa tacaccatca ataaatacag
    10141 acgatgatat agaccagtca ttttcagtaa tttcattcaa tactctattt tttaatcgtc
    10201 tgtagaaagg atattcatct gttttattaa atttctcact aaaatataca gtaagcaaat
    10261 caggaggcca atactctctg gatatatcat caagagagta tccaaagagt ctatatgcta
    10321 aacttttagg aactttttgt tggttaaaat taattgttga aaatatagtt gcttgattgg
    10381 atggtggtaa tgagttataa attgaacaag gtaattgaat atctttattc ttagcatact
    10441 taaatcctaa taatctgtgc tgtccatcga taatagaaca taatcttaga tttatattcg
    10501 gtattattat cttatataat caattctcac tttttttaaa ctcccatttg tcatcttcct
    10561 ttgcaggtgt accatcttca ttatgattga cagagagtat gattgagttt ggaaatgcag
    10621 cctcatcgga gtctatgaat tttgctatgt ctaaggctct tggcatagac tctttcctct
    10681 gcacacctga tatttcccca tcatccattt ccgcctgatt cacataaata catttcaata
    10741 aatcttcggc agacataacc gtctgaaaga attcgccgta tggttgggtg accttaattg
    10801 catggatctc aatagaatta gacatttata gcccctcacc tggctcatca ttagaactca
    10861 tattccttaa ttcatcagac actaatgaat ctggagttac agggatttta aaatcaggct
    10921 taacaacggt aatggaaaac cataatatta tagttataat aagtgatgaa attaacatga
    10981 aattgacgtc attttttaat ccaaaaaata aaaaaacaaa tgatatgaac cataaaaata
    11041 ttgaacaaga tattctaact tgtttacggt gcctttcaga taaagaaata ggattttcgt
    11101 tctcgtcatc tctggataaa cactccgcgg ctaatacgcc caatgtggta ataataaatg
    11161 aaaaaataga aaaacccaac tgcgtattat cttttaaaaa taatgggcca atccatattc
    11221 cgcaggttga tacaaataat gtcaccaaag atataaaaat aaattttggc tgaataattc
    11281 ttggatataa tcctaagata tctttccaca agttcaccca atgtatcata tcatttctcg
    11341 acatgctttt taccctaacc ttgtactatc tacaaaaata atcaaaaaac aagctcccaa
    11401 aacgctctat ctcgcatctt gctaacccgc ttatccccat caacatacag actgaaatgt
    11461 ttttcaagct gccgaatctg ccccattaac gactcctgtt cgcccttatc atccacgttg
    11521 aatacagcct tgatatccgc atttttaatt tcggacacac gatgaataac ccaggttaga
    11581 atatatgaag cgtaattgtt ctctccagtt ttgaagtggg taatttcctg cgttgacagg
    11641 atcgctccaa caccatattc acgaccttcc ttaaggatct tcctcagact ggaaaagtcc
    11701 tggcgcataa agttatctgc ctcatccacc agaatcatct tggtgagctg acgataatcc
    11761 ccctgaatca ctggcttgcc gcgtttttgc atttgtgcat aaaacagatc cagggttagc
    11821 gcaaccacca ggttttgaat ctctgatgag tagccagcca attcgataac tgtgacacca
    11881 tccaccagat catacagact cgtcattttc tctgggtctg tttcaaatat ctgaaaccca
    11941 gctaagttat aaagcgcggc gtacaatgag tcttcttcta ctttttcctg agccagatat
    12001 cgctgccaca catcttctat ggtcggtgct acacggttcc aggttgagcg atcatgaggc
    12061 actattcctg ctaattcata gcactccatg attagcgtaa ccagtttcat acgctgtttc
    12121 acacccaggt tataggcttt ccccattgtt tcagaaaatg cgattgcggt atgtctgggt
    12181 aacatgggca tatcaccaaa cagtgacaaa gggttataag gtagtaacga taattgatat
    12241 ttcttaccgc cagtagcctt cagaaaagcc tcgtccacat agtcagattt ataatcaaag
    12301 atcagtaaac caatcgattt tccatcaacg ttacaggatt ggttacgcat taattgtgtc
    12361 accagcgatt tggtaaattg cgttttcccg gtccccatcg taccgatgat gcctgtgttg
    12421 gtattcataa acttcgtggt gttggtcggt tcccagaata acggggtttg ccgtgtagcg
    12481 tcatgaccaa ataatacttg taagggtaca gtgctgacct ctggtgtcgg ctttggaata
    12541 tcaggtcgtg tgtctggcgg tgtcgttacc tgcactcccg atgctgatgg agatgggtgc
    12601 tcatcactct ctggcttcag acggtattta tctggtaccc ggtagcgttc agccaatggc
    12661 aatttacctt cacgggtagt gattaacgag gatagtaatg aataaggaat ttcgatctgt
    12721 aagatattcg ctgtcgtctc tttataggag agatcgaagc aagatccgct gtctacatgt
    12781 gcaacaacaa agccattagc atagtctttt aactcaccca actgatagtc accggtcaac
    12841 caccattcgc ggcgatccaa caggggagcc agcttatcac tgtccagcac accgtacagc
    12901 tgaagtttct ctacctgcat cagcacctgt cgaataaaga gcgcgcgata aagttgcgat
    12961 gccagtgtat gcggctccag aatatcctgc tgtaaataac gtttaagctc agcagcctgc
    13021 tggcccgcat agttgtaatc tggtcgagcg ccggttttga cttccagcgg cagtaaatag
    13081 agacggtttt ctttaaaacc aacaaagagc acatcatcag agattgcgcc tttacggtac
    13141 ccctgtagat ttcttgataa atcgctttcc ttcatcttta gcccgacgtt accagatacg
    13201 cgaatcattt cggctacaga tagcggtacc cagcagatat cagactcact cagcatggac
    13261 tgtacaaatt tatacgcgcc aatgatgcca tatttctctt tgcgctcttt ttcactggag
    13321 cgcaacatct tcagcaacca ctcaccgtta aaagcattaa attcggcaag tagatgctgg
    13381 ctatcaacgg cagattgccc ggactggctt tcggtctgga gcaggcggag gaatagatcg
    13441 acctgcttcg ttaccgtcac tgcatcgtag cctgcacaac tagtgtactg atcggaatag
    13501 tggatcagca caacatcttt ctggctagta aagaagtcaa gagtaacttt agggtcaata
    13561 atggtggtcc agagtgcact gttgtaagaa taattgagta gctgtttaaa attgccgctt
    13621 acggccaatc caatcccttg tccgtggtat tgactgttgc tctgacgcgc tggctgccac
    13681 aggcagccca ctaaccgggc aagtcgtaga gcgcagtaag attcagtatc aacattccgc
    13741 aggccaaacg cggtaaagta agcatccccc tgtgtttcag ctccttctcc ggaaatcagc
    13801 ccatgacaca gtacaccgct ggcagcatcc tcaatgcgga tctggcggca atccaccgga
    13861 gctgtattgg tgaaaaacgc caaatgtgca taggccaatt tgtcgctttc tgacggcagg
    13921 acaaatttgc tgaacgttaa ccgactgcgt aacaggtcaa tcagcatatc ggcctcagct
    13981 cgccacgcac cgcggttcag gtcaaggtcg tttttaagct gttcatagct gccactctcg
    14041 gcaaagcgat caaacatatt tggcagcagg cgttcatcgt agcaatttac atgcaccgaa
    14101 atagcatgct ctttttcctg cttaaaatat tccactaacc ccagaaacag ttctttcgct
    14161 gttccctgat taatagcatt aataatcaat gcattgtttc cgggactctg aaatagccgg
    14221 gcgtaggctt cggtaaattc attgagcttg tcttttacca aacgttttac gtaatcgtgg
    14281 ctcacctggc gctggggaac gacatcgatc cagaagcgat tttcttccac cggctgcaac
    14341 tgtgcgtatt cgtgttcact gtggtaaacg aatggcatca agccggacac caccagacga
    14401 tcaagcgtaa tggtcggtag tgtggcaaat gacgctgagt cgtattgttc ctgctctgcg
    14461 cagatagttt gtaccagttg taaatggtag gccaacacta ggggatgcaa cggggagaga
    14521 cgttcatgac tgtcgacatt acaaataccc agatgcagca ggcgcttttc ctgcgctgtt
    14581 aacgctctgc tcaactcgat ttgttgaagc gcctgttcaa aggtcgtcac tatatggctt
    14641 accagcgcgc agtattctgc tgaccacgat actaggctag gtaaagtacc gcagcgctgg
    14701 tagtagagga ataattggtc gtaggcgtta tagagatcgg ggtggatggc aacgagttca
    14761 tccagcgcaa acgcgctatc gtcatctcct gtccccaaaa gacgttgatc aactaatgag
    14821 gcttccagcg ccagtaactg ctggcgaaca ccgacaacct tgtgttcggt attatcaaga
    14881 atgatacgac ccttcaatcg gttccaggac gcattcccgt cttctttgaa cagtttattg
    14941 aaacgactct ggtcaaaaag tagcggtaag gttaaccctt cttcggctcc cggcccttcg
    15001 atattgaatg ccagacaaga atcacctgaa acaagcttaa attggatcag atcgctttga
    15061 tttgccagtg tttcaaaatt caccaatgca taatgctggc agtcaatatc accattttct
    15121 tcatccagta tacagacttg gctacccgac tcagcgattc gtagttcgtt gtcttcgcgt
    15181 tggagtgtga tttgtggttt acctggctcg atacggaaac aatgctgaat atcgtctagc
    15241 cagaattgtc cttgtctgac cagtaacagg cggaacttgt actcttcggc tgaattattg
    15301 cgattagtca gttcgaggct aaagaagcag ggattcccat cgaaaggaac cgacgccata
    15361 atacgggagc ttttaccccc tgcacgacta attcgccaga aacgctcttt ttttaactga
    15421 cgatgatgcg caatttttat ctggttatcc tgaagatcgt tgccctgaaa gcaaaactcc
    15481 agctcagttt gcgattgttc gggtggtacc tgtaccagta ggctgatatc gcgcttgcct
    15541 gctttacttg cgcttttcgc ccgttgccaa atcacgccat tttcgaccgt gatatcatcc
    15601 agtaccagtt tttgttcgcg gttctgttct ttttcgttta gataaacagc gaaatcaagc
    15661 tcgcgccagt cgtctttatc attgaaatgt tcctgaataa atttggcgct gaattcagtt
    15721 aatacgttct ccagttgccc actgtagcgt tcaacgctgt cctcgatttg acgaaataac
    15781 ttcctgtttt cattcagtcg ggtatggagc tgtttctggc taaaattcag tagttcatca
    15841 tctttaaaca gcgatagctc ggagaaatcc agattgccgt catccagcag gcgatacaac
    15901 gacgagaagc caaatacggt cgcaccttca tccagtaccg ttgttcgttg atcctttaat
    15961 aagcaacgcg acaactctga acggttgttg tccgtcgtga tcaacttttc caattcatga
    16021 ctgaaggttt cggggtgcca gatagctccc ggtgccgcga cgtcttttgt gctgttaatc
    16081 agggtgtcca gcatgctgtt atgaataatc agcagtgcag ttttggcaaa caccccgctg
    16141 cggccagcaa cttcatcgcg taaatgggat atgtagtttt ccgtgaatgc tggcgctgcg
    16201 gctccgtgca gcaccggaat aagctgaata ccattacagg agagacaggc aaggcgctgc
    16261 tctggcgcaa tttccagatg attgtcacct gccaggtcaa caaaagcctg ccagagttgt
    16321 agagcattgt cagggtcagg agatttaaac tggtaacgct caccaggttg aataatgcca
    16381 ctaacccatt caataaaggt ttccgccaga taggtttcat actgttttac ggacatacac
    16441 ggcgtctcca ctgtcactca ttcgctccac atttcccata cgttcataaa acgctaccag
    16501 cgtttgtgcc gactggttat ccagataaaa tccgcgctgt tcaaagccgc gcagcaactc
    16561 atgcaggcgt agtttgtcat ttttacccac agtgagattg gtcagcagca gcagacgatc
    16621 ctgattcagt accagtactt ttcccgcccg accacgtacc tgaatgaaat cggtgcagat
    16681 ctgggtttca agctcgttga tgtatttccg gttaactgtc gcacgatcag atttcttatc
    16741 ctgaaactgt tcaacagcaa cggttaataa ttgtttaaaa gcttcttcca gcgtaacggc
    16801 ttgcgtatac ttcgatgccg aaagctcgcg tttttcgata aactcctgca aataattatt
    16861 gagatcttgc agtaattgag atgagttatc agaaaaattt aagcaatcct gatagacctg
    16921 ccagagtgga cgcttcttat ctccttttat ttgtagaact tccagcgcgg agagtattgg
    16981 gaaaagtttt tcactttgac gagcgaacca cttataacca aagcgtttta tttgatcccg
    17041 ttcagaactg gctttttcag tgtcgagaat aaagaaaagc gacttacttt gtggctcgcc
    17101 gtcctgccag ttgtccagat tgagcgctag ctgggcacac cagctaaagg catataaacg
    17161 cagagtatta gtcagttctt gtaacaaata ttgagggtgt tctgccaaaa acgccagatc
    17221 agactgaaaa caagtcacca gatagggaag gtagggttgt tcttcagaga atgggccttt
    17281 tcccaataac tttatttttt cattgagctt atttagcatt tgctgctcaa tgaagttcag
    17341 cttatcctgg ataggatatt ccaagctgaa atctcccatt agattagcaa acagagtgcc
    17401 aaggcgttta tcggccattg aagcgccact tattttttcg ccatcaaact gagcatgaaa
    17461 tagtaaaaaa agcggagata cacgaaagat atcttcactg gaaaaataca tccgttccag
    17521 tacagtccag aatgctggtt catcaagcaa ttcttgcaaa tgagccttgc aatcatcgcg
    17581 aaattgactg ggagtatagg catcaatttt acgcttcagg gcgtaactaa gcaccaatcc
    17641 ggtgacaaat tgccagttaa tatcattgtt tttgttacgt atcggcagat aactatccag
    17701 ttgattatta cctactttta acgtcgttgc gatagggtac attccactaa ccctccatac
    17761 cgctaatggt gatcatgtcg tcgtccgctt tagcgcgata aactcgccgc ccaccagcat
    17821 aaaacttgat ttcgctgctg gatttggcct gttcagtaat caaatcaacg atttcgtcca
    17881 gtagaacaat ggcattttta tcgtatttat tggggcgata gccattgttt aatttgttca
    17941 aaagttcaaa taaattaagt ccgatacgaa ctggtaacag agagttttgc ccaactttta
    18001 aatgcacgtc gaagccagta gggtgggccg tatttttatt gcggatggcc tcccagtcag
    18061 gtttcaattc gactggcgcg gttattttta ccccaccata ttcacctaaa aagaactctt
    18121 ctttttgcat actcaattcc ggcgctttgc ggtttgcata gcgctgaata ccagcaataa
    18181 gttcggatgt ataaaaccgg ttcagtgctt ttttctgctc tgagtcagcg atgtaatttt
    18241 tgtgcagatg ccagatttct gaataatgct caaataaaga ttcattgaag aatacggaaa
    18301 atttctgatg ataattattc cccagagatt catcctgcag caaccagaag agccgaatta
    18361 atgaagccgc atcacccgga ttaacgcact ggcgatcaaa tctaatgtgt aatggcgcga
    18421 gtgcagctaa aaaatcatcc agttcagggt caaccaggcc caattcgtag cgcaaaataa
    18481 actgatcgat ttcataggtg tgcagcctag caggatcaaa atcagaaact tttttaatca
    18541 ggtcgttttc tgccccagtg aaaagattat caaataaata ccccggcccc ataagcaaat
    18601 gatgcagaaa atccagtaga gttcgggttg tcacaaactg atctttaatc aggcgggcct
    18661 taaagagctg agtgatgaga acatcctgaa cacccggcag acaaagtaat ttatagttag
    18721 caacttcttt cagctcaggg ttaacggtct catcatgtcg aaaaataaac tgaaacaaat
    18781 tactatcatc attacgggtt aaattatcca gtaatgtttt aataaacgat gaatactgtt
    18841 ttttttcatt aaactggaat ttagggtaat gctcaaagtc aaaaaaagaa caatgcccac
    18901 tgcggtatgg gcggctttct tcctgatctg ctgacaagaa agaatcgatc gcagtgcgga
    18961 tcgccagatg gcattcagca ccttctctgg caaaattggc gagcatcccg gtattgatgc
    19021 ctataagcaa aggagatgag tattggtgat gattgctaaa taaatcattt aaagcatcaa
    19081 tagctgattg ccggggagca aagctgtgtg ttgcatccag atgaaaacta aagcgctgct
    19141 gataacgagg gtttgactta caacgggtca aaatttcgga ctttccatca ccactactgc
    19201 cacacaaaaa gatgatttca ccgggcttag caacatccag ataacgttgc agctcagttt
    19261 caatttcttg tttaacaaag agttgttctt ttaactgatc gaactcatcc ttttgacgat
    19321 gcgttaccgt ggttacggaa aacgaagatg acttcgcaag tacacccaac gctttacgta
    19381 atgtgattgc gctcataact tcatgttctc aaaagtagga ttatacctat tctataaaag
    19441 ctatccgctg atatctatag gcactttgag acatttatta cgggcaattt taaaattgta
    19501 tcttttccat ccgtacacaa aacacatcca gagatggcgg ggaaaatcca tctatcaacc
    19561 agttccactt tggaaattgc ttgattagta gtatcttaat caaacacata cgctaaatat
    19621 tacccccgat tgattttcgc atagtcaaac agcgtaatcg atctctccct attcgcatcc
    19681 agaaaatcag cccaccatac tgcaaatcag ctcgcgctca tccagatgtt ctgccttatg
    19741 gatatacgcc cccttaccga tgtgactcat ctgacgttca gccgctttat ctcatgttgt
    19801 tctggtctca atacttccat gccgttgcaa ttacctctta aaccagccct taccattttt
    19861 atgggtacga taagaaaact ttatcgggca cgatcgattt tatcgatcga ttcgatcgaa
    19921 aacattcctt ttaccttatc tttttcatgg ttttagaatg agcaacccca tttatcgttc
    19981 ggggaaagtg atagcggaga tgattgaaat gaataaggtt attgcggttt cagcgcttgc
    20041 catggcaggc atgttttcga cccaggctct ggctgatgaa agcaaaacag gcttttatgt
    20101 gaccggtaaa gcaggtgctt ctgttatgtc acttgcagac cagcgtttcc tgtcggggga
    20161 tggagaggaa acatcaaaat ataaaggcgg tgatggccat gatacggtat tcagtggcgg
    20221 tatcgcggcc ggttatgatt tttacccgca gttcagtatt ccggttcgta cggaactgga
    20281 gttttacgct cgtggaaaag ctgattcgaa gtataacgta gataaagaca gctggtcagg
    20341 cggttactgg cgtgatgacc tgaagaatga ggtgtcagtc aacacactga tgctgaatgc
    20401 gtactatgac ttccggaatg acagtgcatt cacaccatgg gtatctgcag ggattggcta
    20461 cgccagaatt caccagaaaa caaccggtat cagtacctgg gattatgggt acggaagcag
    20521 tggtcgcgaa tcgttgtcac gttcaggctc tgctgacaac ttcgcatgga gccttggcgc
    20581 gggtgtccgc tatgacgtaa ccccggatat cgctctggac ctcagctatc gctatcttga
    20641 tgcaggtgat agcagtgtga gttacaagga cgagtggggc gataaatata agtcagaagt
    20701 tgatgttaaa agtcatgaca tcctgcttgg tgtgacttat aacttctgac gacactgctc
    20761 ctgaacgata atacgtatat ttagcaatta agataattgc gtatcttctg caattaaaca
    20821 gaaataccct gcagtctatt actgcagggt tatctgtttc acagataatt tctgattttt
    20881 attatcctgt tctcttcatc ataatgttgt cgatatactc cgatgaatat cagaaaactg
    20941 ttttgtccgg gaaacacacc ccggatttta ttgtttttat tcttttttgt tgtttctgca
    21001 ataaccacaa ttgcatgcgg atacactgag aagaatgcca caggaaatgt gctgcttctg
    21061 tttctccttc ttctccttgc acacagaaat accctcacat ccattacagc gctgttattt
    21121 ctgttctgtt gtgcactgta tgcgcctgcg ggtatgacgt acggtaaaat caacaacagt
    21181 tttattgtcg cgttgctgca gaccacggct gatgaggctg cagagtttac cgggatgatt
    21241 cctgtttatc attttctggt cagtgccgcg attctggtat tcatggtgat tttctggcgg
    21301 acacaccacc gcggtcaccg taactggcta gcactgctgc tattcgtatt atgctctgta
    21361 aacagctggc cgttgcggat ggttaaagga actgttgtgg ggacaactga cacattgcgt
    21421 gaaatgcagc gttataaaca actgagtcag cacggggctg ataactggaa aatcctgccg
    21481 ggtgtgccgt tgtatgacac gattgttatc gttactggtg agagtgtgcg cagggattat
    21541 atgtcggtat atggctatcc cgtgccaacc acgccgtggc tgaatactgc tcccggttta
    21601 tttattgacg gctatacatc gacagcagcc agtaccgtac cttccctgag ccggacactg
    21661 atttatgact atgagcagaa ccctgactcc ggcaacaacg tggtggcgct ggcagcaaaa
    21721 gcaggataca gcacatggtg gatatccaat cagggaaaac tgggagagca tgacacacgc
    21781 atctctgtta ttgcttctga tgcggagcat accgttttcc tcaagaaagg cagcttcgct
    21841 tcccgtaaaa cggatgacat gttgttgtta caggaaacag aacgtgcgct ggcggataaa
    21901 tcctcgccga aggtgatttt cctgcacatg attggctctc atccgaatcc gtgtgaccga
    21961 cttaactcct ggccgaatta ttacctggag cagtatcccc gaaagattgc ctgttacctc
    22021 gccagcatca gtaaactgga taactttctc ggtcagcttg atggtatcct tcgccggcat
    22081 tcccgtcact ttgcaatgct ttacttttct gaccatgggc tgtcggtcag cgacagtgct
    22141 aatcctgttc atcatgatgg tcatgtgcag gggggctaca gcgttcccct gattattacc
    22201 gccagtgaca tcacgtctca tcagtccgtc agcaggaaaa tcagtgcccg taatttcgca
    22261 ggcatttttc agtggctgac cggtattcgt accgaaaata taacgccatt caatccgctg
    22321 acagacgaag ataatgaacc cgttatggtt tttaacggag agaaaaatgt gccggcagac
    22381 agtctgaaac cgcagccact tattcttccg gaccacaggt aatcatatat ggcaaactgt
    22441 aatacagtct cttgtactga aaaggattct gtatagaata gaacctgtat tcatatcagc
    22501 acaagggacc accaaatccc ggcaacattc cttacgtcag ttatcccgca tcaccctgtg
    22561 ggtgcgggat tttttatgat gtttcttttt attgaatata tataaataac gaataactgc
    22621 ggattctgat aatccacctg cagggagcaa tccggtaaac atttaataca tcagttaata
    22681 tcgttttgcc tttttaacaa agaaaatatt catggagtaa gttcccgggc atttgcccgg
    22741 gatgattact gttttaatgg attattaatc tttgcatatt caaatggact gataaatttt
    22801 tctctgtttg catccagaaa atcggcccac cattgtagca tcaagcggcg ttcttccaga
    22861 tgctctgctt tatggatata cgcggccctc actgaatttc gctccatgtg gctcatctga
    22921 cgttcaacag catcacgaga ccacaaacct gattcgacca atgaactaca ggccattgtt
    22981 cgaaagccat gaccacaaac ctctactttt gtatcatacc ccatgacccg taacgcacta
    23041 tttaccgtat tctcactcat gggtttgtgc gaatcgtgat caccaataaa tatcaagtca
    23101 tgggccccac aaaactgttt tatctgcttt aaaattgcaa gagcttgcgt tgaaagaggc
    23161 actagatgcg ttgtacgcat ttttgagcct ctatgggaat gtttcactcc aggaataggc
    23221 tcccgctccg gtgggatagt ccatatagac gcttcgaaat cgatctctga ccaacgagca
    23281 aaacgcagct cactggaccg aataaagatc agcaaagaga gttctgtcgc ccatcgggtt
    23341 agcggcctac cagtatagct atctattttt gtaagcaact cagggatgcg ctttaattca
    23401 agcgcgggac gatgttgtcg attacaggaa gcaaccgccc cagccatctc ttgtgccggg
    23461 ttataatcaa ttaacccact ttgcactgca tagcgcatga tggctgtagt gcgctgctga
    23521 agacgagcgg ccacttcaag acgtccagac atttctacgg ccttaatggg tgctaataaa
    23581 tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag attatcttcc
    23641 agacttttta gcacacgatg ggcgtgatct tcagaccact ttttattggt gccatgccac
    23701 tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc atttttcttt
    23761 ttgtctcccg gatcgacgcc attcgcaagc agcttacgcg cctcgtcacg acgtactctg
    23821 gcatccgcta gtgtgatttc aggataaacc ccaagtgcca gcattttttg ctttccctca
    23881 taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag atgcataccg
    23941 ccaccatcag tcagcttata ttgcttatcc gttggcttgg ctgctctgac tttgatatct
    24001 gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg gaaataccat
    24061 catctgtacc aacatatgtt ggtagatgta cgttgatatc ggttgacctt gagagagtta
    24121 atatagcggg aaaggtagat aaacactgga tttcaggcat aaaaaaagac ctcagttgag
    24181 gtctatttac atacttttgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt
    24241 tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga
    24301 aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc
    24361 gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt cacactccag
    24421 gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga ccaaaaaatg
    24481 tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac
    24541 gatcccggtg agctgaagcc acaagatgtt gaagtgcagg tggattactg cgggatctgt
    24601 cattccgatc tgtcaatgat cgataacgaa tggggatttt cacaatatcc gctggttgcc
    24661 gggcatgagg taattgggcg cgtggtggcg ctcgggagcg ccgcgcagga taaaggtttg
    24721 caggtcggtc agcgtgtcgg aattggctgg acggcgcgta gctgtggtca ctgcgacgcc
    24781 tgtattagcg gtaatcagat caactgcgag caaggcgcgg tgccgacgat tatgaatcgc
    24841 ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgattccact gccagaaaat
    24901 attgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt taaaccactg
    24961 ttgatgcacc atatcacagc caccagccgc gttggggtaa ttggtattgg cgggctgggg
    25021 catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt tagttcgaat
    25081 ccggcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat
    25141 ccgcaggcac tcaaagcact ggcggggcag tttgatctca ttatcaacac cgtcaacgtc
    25201 agcctcgact ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc
    25261 ggtgcggttc tcacgccgct gtctgttccg gcctttacgt taattgcggg cgaccgcagc
    25321 atctctggtt ctgctaccgg cacgccttat gagctgcgta agctgatgcg ctttgccgcc
    25381 cgcagcaagg ttgcaccgac caccgaattg ttcccgatgt cgaaaattaa cgacgccatc
    25441 aagcatgtgc gcgacggtaa ggcgcgttac cgcgtggttc tgaaagccga cttctgatcc
    25501 ctttgccggg tgcctgtcgc ccggcactaa aatctctttg gaaggcatct cgcaatctgc
    25561 tgaatttagc ggtgaagagt gcgcagccgt tgcctatact ccaggcagga aactggagga
    25621 aatctcatga gcgaacccct gttaattgcc cgcacgccag acacagaact gtttttactg
    25681 ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg taaaaccgtt
    25741 acgctgcaaa aactggcaga gtcattgtcg gaaatcggcg tgccggtgtt tatggctgat
    25801 gtgaaaggcg atctgaccgg tatcgcgcag gcaggaacgg cgtcggaaaa actgctcaca
    25861 aggcttaaaa atatcggcgt caatgactgg caaccgcatg ccaatccggt ggtggtgtgg
    25921 gatatctttg gcgagaaagg ccattcggta cgggcgacgg tttcggatct ggggccgctg
    25981 ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa tatcatcttc
    26041 cgcattgctg atgatcaggg gctgttactg ctcgacttta aagatttgcg ggcgattacc
    26101 cagtacatcg gcgataacgc caaatccttc cagaatcagt acggaaatat cagtagcgca
    26161 tcggttggtg ccatccagcg cgggctattg tcgctggaac agcaaggcgc agcacacttc
    26221 tttggtgagc cgatgctgga tatcaaagac tggatgcgca ccgataccaa cggtaaaggc
    26281 gttatcaata tcctcagcgc cgagaagctt tatcagatgc cgaaactgta cgccgccagc
    26341 ctgctgtgga tgctttcaga gttgtatgaa caattgccgg aagcgggcga tctggagaaa
    26401 ccaaaactgg tgtttttctt cgacgaagca catctgctgt ttaacgatgc cccgcaggta
    26461 ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt gggcgtctgg
    26521 ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcggccagct cggtaatcgc
    26581 gttcagcacg ctttgcgggc ttttacgccc aaagatcaga aagcggtaaa agctgcggcg
    26641 caaaccatgc gggccaatcc ggcatttgat accgaaaagg caatccagga actggggacc
    26701 ggcgaggcgt taatctcgtt tctcgatgca aaaggaagtc cttcagtggt ggaacgagcg
    26761 atggtgatcg cgccttgttc gcgaatgggg ccagtgacgg aagatgagcg taatggcttg
    26821 attaatcact ccccggtgta tggcaagtac gaggatgacg tggaccgcga gtccgcctat
    26881 gagatgctgc aaaaaggcgt tcaggccagt accgagcagc aaaagaatcc ccccacgaaa
    26941 gggaaagagg tggcggtgga tgacggtatt cttggtggat tgaaggatat tttgtttggc
    27001 actaccggac cacgcggcgg gaagaaagat ggtgtggtgc aaacaatggc gaaaagcgcc
    27061 gctcgccagg tgacgaatca gattgtacgc gggatgttgg ggagtttgct gggggggaga
    27121 agaaggtaag tcaggcgcga tctgtccccc tcaccctaac cctctcccca aaggggcgag
    27181 gggaccgatc gaactcgtat ttgactttat caacaatgtg aaagggagct ttcgctccct
    27241 tgcttggtta cgattttctc attaacagcc acaggctgat taagaagaag ctggcgcttg
    27301 gcaacagtgc gccgataatc ggcgggatgc cgtaaaccaa cgtcagcggg ccgaagatct
    27361 ggtccagtac gtagaagaca aaaccgaagc tgataccggt gaccacacgc acgcccatcg
    27421 gtacgctacg cagtgggcca aagatgaacg acagcgccat cagcatcatc accgccacgg
    27481 atagcggctg gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccac
    27541 tcgacttcag atacttcacg tagttgtgca aaccgctgat tgagagtgca tccgggtcca
    27601 gcgccaccac gcccagtttg tctggcgtga ggttggtttt ccaggtgccg ctcaccgtct
    27661 gcgaaccggt aatctgtttc ggattggtca aatcagattc atcaacctgc gacagacgcc
    27721 agactttatg ttccgggtca aacttcgcgg tagcggcata gcgtacggat tgcagacgac
    27781 gattctcgtt aaaggcataa atgctgatgc cacctaactc ttcgtcacct ttaacccgct
    27841 caatgtagac gaagttgttg ccatctttcg cccataagcc ttgctgggta gagagcaacg
    27901 agccgccgta catcgcctgc gcacggtagt tacgcgccat ctgttcgccc tgcggcgcga
    27961 cccattcgcc aatcgccatc gtcagcaaga ccagcggaat ggcggttttc atcaccgaca
    28021 gcgccacctg catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg
    28081 ccagcatccc aagaccaagc aacgccccaa gcagagccgc catcgggaag aagatttgca
    28141 catctttcgg cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg taactcccct
    28201 gcccggcttt tttcagctga tcgacaaatt tgataatgcc cgacagcgac accagcatga
    28261 acagcgtcat catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa
    28321 aaggttgcat cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac
    28381 ggtgtcccaa aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat
    28441 ccacagtgtc gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag
    28501 gaagaaaagt agatacagca gcatggctgg cagcatcgac agtacgcgtc cctgacgtgg
    28561 gttaaccacg ctcagcggta cgaccataag tgccatcata aacacggtga ataccaacgt
    28621 gatacgccag ttcagttctg cgcgggcacg atcggtgtca gtgttccaca atgtacgcat
    28681 gtccatctgg tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg
    28741 ataatcctgg aagtccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt
    28801 tccctggttg agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc
    28861 ggccaccacc acagaaggac gtgcattacc ttttggtcga atttgcgcga ggaacacatc
    28921 tttgaaatcg ctgccgtcaa cgctttcgat gaacagcacc gaactgccat tagtcgcttg
    28981 ctggaattgc ccttgcgcca gcgccgccat gccagggttc gctttcgctt ctgctaacac
    29041 ttcatcctga tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat
    29101 tgccgtgaat accgcaagga tcattgccgc tttcaccaga accgctttgc tcaggccgca
    29161 ggcatgcatt accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc
    29221 gaggaacagg cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag
    29281 ggagagcacc agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa
    29341 cttttgacag aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt
    29401 ctcccgcacc agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt
    29461 gcaggatttt agcttgtttc atgacttaaa cgtcatttat tctcttgagt cgtcgaaatc
    29521 gtcgctaaga taattatact caacggattc acctctcaga ttttgttctg acgtgccaat
    29581 gccgtaataa cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag
    29641 cttgcaattc tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg
    29701 agtttagtgt aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg
    29761 tcttcgaacc acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt
    29821 acatcagcgc cctgctacgt cggggcgaac tggaaggaaa accggggcag acattgttgc
    29881 tgcaccatgt tccgaatgta ctttccgagc gaattctcct tattggttgc ggcaaagaac
    29941 gtgagctgga tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg
    30001 atactggctc aatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca
    30061 actactggaa agtgcgtcag gctgtcgaga cggcaaaaga gacgctctac agtttcgatc
    30121 agctgaaaac gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc
    30181 cgacccgccg tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg
    30241 ccgggattaa agcagcaaaa gatctcggca atatgccgcc gaatatctgt aacgccgctt
    30301 acctcgcctc tcaggcgcgc caactggctg acagctacag caagaatgtc attacccgcg
    30361 ttatcggcga acaacagatg aaagagctgg ggatgcattc ctatctggcg gtcggtcagg
    30421 gttcgcagaa cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg
    30481 cacgcccaat cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctcgatca
    30541 agccttcaga aggcatggat gagatgaagt acgatatgtg cggtgcggca gcggtttacg
    30601 gcgtgatgcg gatggtcgcg gagctacaac tgccgattaa cgttatcggc gtgctggctg
    30661 gctgcgaaaa catgcctggc gggcgtgcct atcgtccggg cgatgtgtta accaccatgt
    30721 ccggtcaaac cgttgaagtg ctgaacaccg acgctgaagg ccgcctggta ctgtgcgacg
    30781 tgttaactta cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg acgctgaccg
    30841 gtgcctgcgt gatcgcgctg ggtcatcaca ttactggtct gatggcgaac cacaatccgc
    30901 tggcccatga actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc
    30961 tgggtgacga gtatcaggaa caactggagt ccaattttgc cgatatggcg aacattggcg
    31021 gtcgtcctgg tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtaca
    31081 actgggcgca cctggatatc gccggtaccg cctggcgttc tggtaaagcg aaaggcgcaa
    31141 ccggtcgtcc ggtagcgttg ctggcacagt tcctgttaaa ccgcgccggg tttaacggcg
    31201 aagagtaagt ttgcgtttta ccctcaccct aaccctctcc ctaagggaga gggaaccgat
    31261 cgagcgcaat agaaattcat tgctcatctt ttagggtgag gaatgcagca atatttaaat
    31321 ccaccacaag aagccccatt tatgaaaaac gcgacgttct accttctgga caatgacacc
    31381 accgtcgatg gcttaagcgc cgttgagcag ctggtgtgtg aaattgccgc agaacgttgg
    31441 cgcagtggta agcgcgtgct catcgcctgt gaagatgaaa agcaggctta ccggctggat
    31501 gaagccctgt gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga
    31561 ccgcgcggcg gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc
    31621 gatatattga ttagtctgcg aacaagcttt gcagattttg ccaccgcttt cacagaagtg
    31681 gtagacttcg ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc
    31741 taccgcgtgg ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacatata
    31801 acccacaaga tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc
    31861 ctaatggcga tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg
    31921 gcagtttgca tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc
    31981 agcgcatgca gggcaaaaac accctgtggc aggtcggtac tgaccacgca gggatcgcta
    32041 cccagatggt cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg
    32101 gccgcgaagc tttcatcgac aaaatctggg aatggaaagc ggaatccggc ggcaccatta
    32161 cccgtcagat gcgccgtctc ggcaactccg tggactggga gcgtgaacgc ttcaccatgg
    32221 acgaaggcct gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga
    32281 tttaccgtgg caaacgcctg gtaaactggg atccgaaact gcgcaccgct atctctgacc
    32341 tggaagtgga aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg
    32401 acggcgcgaa aaccgcagat ggtaaagatt atctggtggt tgcgactact cgtccggaaa
    32461 ccctgctggg cgataccggc gtggccgtta acccggaaga tccgcgttac aaagatctga
    32521 ttggcaaata tgtcattctg ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac
    32581 acgccgacat ggaaaaaggc accggctgcg tgaaaatcac tccggcgcac gactttaacg
    32641 actatgaagt gggtaaacgt cacgccctgc cgatgatcaa catcctgacc tttgacggcg
    32701 atatccgtga aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca
    32761 gcgaaatccc tgcagagttc cagaaactgg agcgttttgc tgcacgtaaa gcggtcgttg
    32821 ctgcggttga cgcactcggc ctgctggaag aaattaaacc acatgacctg accgttcctt
    32881 acggcgaccg tggcggcgtg gtcatcgaac caatgctgac cgaccagtgg tacgtgcgtg
    32941 ccgatgtcct ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt cagttcgtac
    33001 cgaagcagta cgaaaacatg tacttctcct ggatgcgcga tattcaggac tggtgtatct
    33061 ctcgtcagtt gtggtggggt caccgtatcc cggcatggta tgacgaagcg ggtaacgttt
    33121 atgttggccg caacgaagac gaagtgcgta aagaaaataa cctcggtgcc gacgttgccc
    33181 tgcgtcagga cgaagacgtt ctcgacacct ggttctcttc tgcactgtgg accttctcta
    33241 ccctaggctg gccggaaaac accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg
    33301 tatccggctt cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact
    33361 tcatcaaaga tgaaaatggc aaaccgcagg tgccgttcca caccgtttac atgaccggcc
    33421 tgattcgtga cgacgaaggc cagaagatgt ccaaatccaa gggtaacgtt attgacccac
    33481 tggatatggt tgacggtatt tcgctgccag aactgctgga aaaacgcacc ggcaatatga
    33541 tgcagccaca gctggcggac aaaatccgta agcgcaccga gaagcagttc ccgaacggta
    33601 ttgaaccgca cggtactgac gcgctgcgct tcaccctggc ggcactggcg tctaccggtc
    33661 gtgacatcaa ctgggatatg aagcgcctgg aaggttaccg taacttctgt aacaagctgt
    33721 ggaacgccag ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc ttcaacggcg
    33781 gcgaaatgac gctgtcgctg gcggaccgct ggattctggc ggagttcaac cagaccatca
    33841 aagcgtaccg cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc attctgtatg
    33901 agttcacctg gaaccagttc tgtgactggt atctcgagct gaccaagccg gtaatgaacg
    33961 gtggcaccga agcagaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc
    34021 tgctgcgcct cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga
    34081 aagtactttg cggtatcact gccgacacca tcatgctgca gccgttcccg cagtacgatg
    34141 catctcaggt tgatgaagcc gcactggccg acaccgagtg gctgaagcag gcgatcgttg
    34201 cggtacgtaa catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctac
    34261 tgcgtggttg cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa
    34321 cgctggcgcg tctggaaagt atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg
    34381 ttactaagat catcgacggt gcagagctgc tgatcccgat ggctggcctc atcaacaaag
    34441 aagatgagct ggcgcgtctg gcgaaagaag tggcgaagat tgaaggtgaa atcagccgta
    34501 tcgagaacaa gctggcgaat gaaggctttg tcgcccgcgc accggaagcg gtcatcgcga
    34561 aagagcgtga aaagctggaa ggctatgcag aagcgaaagc gaagctgatt gaacagcagg
    34621 ctgttatcgc cgcgctgtaa tcaaaacaca gtaatgaaaa cgaaggccgg agcatgctcc
    34681 ggcctttttt atctcttaca gaaatggtaa agaaggcata attccccgtg aaatcctggc
    34741 taagaagcct ttatctggtt gttcttcttt attgattaaa ggaaggtcat ccaaatcgcc
    34801 cagcaaatac gtattttttg cctgccagtc aatcatccat attttcagca gtggataagc
    34861 cagaccgccc gtaatcccgg atatcaccac caacgcacac atgcgataaa gcataccgtg
    34921 gtacgttaaa gttgagcgaa aacgaatacg cccatcattc agtgacaggt tgctcataaa
    34981 atgatttcgc aacgacaccg ttaaataact ggtgctaaca gcaatcccaa aataatagat
    35041 taactgcgcg attatcattt tacgttgcat ttccataaat tgctgtaaat tctcaatatc
    35101 atcatttgca tacacagaac tatcatacgc atttaatatt tgatcgaaga taatataacc
    35161 agcaacagcg agaaaaggta ataaagctaa aaatgcgaga atggcatatt ttatacaata
    35221 cgtagtatct aatttcacct tgaaacgatg tataccgaaa ctggtattgc tccagagaaa
    35281 actcattacc aggctatata aagtaccatt aaaaatacca atggaaacaa taccaactat
    35341 tgccatcaga accacagata taataacact acttgaacta ttggcatgta gcatctttgt
    35401 cgagataaag aaaacagtcc ccatcccaat ggccattaaa atcggcaaga aaaaggttac
    35461 ccaccagaac cctttcatag agcaattaaa actaaatctt acgccgttga gactggtcat
    35521 caaggcctga taacgcagtc cttttgctgc cataaatata agcaaaacca acagtaacaa
    35581 agtcaaaaca cagccaacaa gtggcatatc tgctgacact gtcattaaga ttgcgaaata
    35641 gcaaaaaaca aaaacaagac aactaacaaa aacattccca ccagtaattc cataagaaaa
    35701 tcgttgtccg ttaacttcca tattagcata gagataacgc ttacatttca ttaatgccca
    35761 tggcaaatag atacctagcg ttataatcgt taacaacaca ttcaccacac aaataagaaa
    35821 atatctctca cctgtaccat gaaaaacaaa cgaatgggac ggaacatcca tttcattaat
    35881 aacttgagcc ataaaaattc cctgaattaa aaaatccatt gatataattt atatatacag
    35941 acagtaaaat gttctgccac gttacaatac tcttatcagt aataggctgg caaaaacttt
    36001 attactttat aagtcgcttg cgcacgtaag aagagagtgg tattaaacaa atgtataatt
    36061 actttatttt tattgggtta aactatgaat aacattgcgc cgcaatcacc tgtaatgcgt
    36121 cgcctgacgc agcaggataa tcccgccatc gcccgcgtca ttcgtcaggt atccgccgaa
    36181 tacggtctta ccgctgataa aggctacacc gtcgccgatc cgaacctgga cgagttgtat
    36241 caagtgtata gtcagccggg tcatgcatat tgggtggttg agtacgatgg tgaagtggtc
    36301 ggcggcggtg ggattgcgcc attaaccggg agtcagtcgg acatttgcga actgcagaag
    36361 atgtattttc ttccggctat tcgcggtaaa gggctggcga aaaaactggc cttaatggcg
    36421 atggagcagg cacgagagat gggcttcaaa cgctgctatc tggaaacgac cgcttttttg
    36481 aaagaagcga ttggtttgta tgaacattta ggttttcaac atatcgacta tgcacttggc
    36541 tgcacgggcc atgtagattg tgaagtgcgg atgctacgcg aactctaaaa aaatgcccgc
    36601 tagtgtgtgc tatcgggcat tttaatcaat taattagtgg cgaaccccgt catcgtcttc
    36661 atcgacaaaa tcttcatcgt cgccgtcttc gccattggga tcttcaaagt aagtgcccca
    36721 accgtcgtac tcaacgtcaa atttctccgc caacgtcatc agttgttcaa cctgggcatc
    36781 gatcagatcg gcattcaacg cgcactcgct gaggatgtcg cagcaaatca cgatatcacc
    36841 atcttcaact tccagctctt ctggatcggt cacttcgtaa ccgagtttaa acgcttcaac
    36901 tgctgctttt tccagggttt ccagatcgtc tgcggaaaga tggtgttcga tggtgtacag
    36961 cgcgtccgga tcgctgccat cttccagtaa ttcttcaata atcaaacgtg tttcttcacg
    37021 ctgttcttcc agttgttccg ggtttgccat ggctcattcc tcttaatgtc ctgccgatac
    37081 tcttattgtc acacaccact gacattgcct ccacctttgt gacaaagatt tatgctttag
    37141 acttgcaaat gaataaccat ccatataaat tgaattttaa ttcattaagg cgttagccac
    37201 aggagggatc tatgtccggg ttttatcata agcatttcct gaaattactc gatttcacgc
    37261 cagctgaact caacagcctg ctgcagttag ccgcgaagct gaaagccgat aagaaaagcg
    37321 gcaaaaaaga agccaaactc actggtaaaa acatcgcgct catcttcgaa aaagactcga
    37381 cccgtacccg atgctctttc gaagttgccg catatgacca gggtgctcgc gttacttatc
    37441 tcggcccaag cggcagtcag attggtcata aagagtcgat taaagacact gcccgcgtgc
    37501 tgggtcgcat gtatgacggt attcagtatc gcggctatgg tcaggagatt gtcgaaacac
    37561 tggcggaata cgctggcgtg ccggtatgga acggcctgac caatgagttt caccccacgc
    37621 agttgctggc ggatctcctc accatgcagg agcatttacc cggtaaagca ttcaacgaaa
    37681 tgacgctggt ctatgcaggt gacgcacgta acaacatggg caattcgatg ctcgaagctg
    37741 cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg ccggaagctg
    37801 cgctggttac ggaatgccgc gccctggcac agcaaaatgg cgggaatatt acgctgacgg
    37861 aagatgtagc caaaggagtt gaaggtgctg actttatcta taccgatgta tgggtgtcga
    37921 tgggggaagc aaaagagaaa tgggcagaac ggattgcatt actgcgtgat tatcaggtga
    37981 acagcaagat gatgcagttg accggtaacc cggaggtcaa attcctccac tgcctgcccg
    38041 cgtttcatga cgaccagacg acgcttggta agaaaatggc ggaagagttt ggcctacatg
    38101 gcggaatgga agtgactgat gaggtcttcg aatctgccgc cagcattgtt tttgatcagg
    38161 cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc gacgctcagt aaataattga
    38221 tatcgctata aagatgccgg gaaatacaaa aacatcccgg catcacatta ttttatgcag
    38281 taatagttca attattccat ccatattttt ctattgctat acgtaaatct tccggaggat
    38341 tttcatcatt cttgttctaa tttttaattc attaaccatc ttttcttcaa aaatcttaac
    38401 gacataaccc ggattgtttt ttacaatttc atttgttttt tcttcattac cataaacata
    38461 tcctaactca ttaagataca gctgttcgcc ctcataatct ttaagtaatt gatagaaata
    38521 ccacttagca aaatcaggtt gcttcatgaa aaaatccttc aaaaaaaata tcatttacta
    38581 aaatgctgac aggcacttca ttaaatagca acttaacatc atcaaataac cttagtatta
    38641 agcgtgattt ttcttgattg cttaagttaa aatcattgac atatgcaacc aacttatccc
    38701 aaatcaattc ctgtaattta cggctatttt tatagtgttc tttatcgaga ttgaaaaata
    38761 aggtctcagc tattttaatt ttagattgat cgcagctaaa atctttagat tgatcgcggc
    38821 taaaatcaat atgatttatc acggagcatg catactttat cttttcagaa tcaccaaaat
    38881 attgcattct tttattaaaa caccaatcca gtattcttac atcattaata tatgggaatt
    38941 ttaaaaaaat ctccagaatc gaatcgatga ccggtgtaag gttaactttt gcatcatcta
    39001 aggaatcaat aagaatatga gacaggttaa ctttgatgtc attaatttca ttgtcagtag
    39061 ctttcttatt attaagaaga taattattta ctgcgttaag gtcatctatt tctttcagta
    39121 tccctttttc accaagcaag tatgaaatcg attcattaca tacataaggc attaatagca
    39181 gatgttcttt ccttattgct aataatgcat tattcaatgg tttttctaat aaatccgtaa
    39241 tcgtagaatc aactgaatct ttacaactca cctgcgagtt attcaagtcc tgagaattat
    39301 ttaactcctg cggaatattt aatagacgag ctatttcctt gttagcttcg cgttggtaac
    39361 acgccagaat cttgttagta atcaggtgta atgaactctc atcttttctt tcaaaggact
    39421 tcccttcata acgattagtc gaaccatcag gttgtgtaaa tataatgact gtatgtcctt
    39481 cacgaatccc attattaaaa gctgaggcaa gtgtaatttc tatttccacc ttagataaat
    39541 ccgctcgctc cccttcagga ttattcataa caaacttcat tatctcacta ccatcattat
    39601 ttttgatata agatgaggac ttaatatgag ccatcatatt tctacccgct tcgttggagc
    39661 gtttgctaaa atccacataa cccagggtaa atatcttagc caaaaactcc aaaaatgctc
    39721 tgggagaaag aatattgctt ctgctattct gccctccggc aattcgggca aaatcattca
    39781 ttcccacata ccaggtatct aagtcttcat ttttttgttt acaattgtcg gctgatgtaa
    39841 tgttttgaga aaaattccaa cctgatattt tgctcatgac ggactccttg agttaataag
    39901 aacgtctaag cattacaaat tttactcaca aaaaaatatc aaaaaacgct cttttcacaa
    39961 actcgccgta aatctggagt tatttacggc gaaaagtcat cacgccatca gcatcttcac
    40021 tactgctttg cgaacctggg ctggtgcacc cactgcgcac agcggtttat gcacttcccc
    40081 cggataaaac acaacaaaat caccttcatt aaggataact gttttctcat caacgccttc
    40141 cggcaaaaat gcgatgtctt tatcagctaa ccagtcggta tccggcgtgc ctgcaggttg
    40201 cgtgctgaag gtcatgcctt cctgaccttt taacacaatc tgaatgtcga gatagcgggc
    40261 atggtactcc gcacggcggg cttcgtacgg ctcggtcata tcttccgaga taagataaaa
    40321 cagacgattg ccttcgatat cgtgcttgcc ctttggcgtt tctgccgtaa cgtgtgcttt
    40381 gatatgctca atcgcctggc gtaactcctg gggtagccac ggctgaagat tatgaatatt
    40441 tccgatgatc atcttttcca ctccttaatt aaaacaacgt ttcatttcta tcgttatacg
    40501 aaaagatgat caattccgct agccttaacg cgtgaattat tgtcttatca caacttatcg
    40561 gcaggcgcgt tcccgccttt cggatcaagg ccagcatcac accttgagaa gagtttttat
    40621 cgcttccccc gccagtgcag ctgttgcaca gagttgttcg attgagtgcc cctcccgtgc
    40681 cttcgcagaa agccctgaca tcacggtact cataaaatca gtcacacatt gtgcgctttg
    40741 tggatgccgc ctggcgatat agtcataaat ggtcgtttct gcggcgtgat aatattgaac
    40801 ggcaatatca cgcgctagtg gatcatgact atgaatacct tcaagaacca tacagccagc
    40861 gcagccgccg ttttggctat atctgcgcgc cgcttctttt aatacctcaa ccaggcactc
    40921 gcctactgga cgatcatcac gaagaatatc ggcaagcgga atagcttccg taccgacgta
    40981 ttcattgagt acacggctaa ataacccagc tttactgcca aaagccgcgt agaggctcgg
    41041 ggggttaata ccaagataat cagtaacttc agcaacactg acagcatcga aacctttttg
    41101 gtgaaaaagc acttttgccg cagagattgc ctgctcagga gcgaaacgtc tgggacgacc
    41161 tggaacgcga gattgttttt tagtgaccat tacaaaacct gttgacagaa agttaaaaca
    41221 gttttgtaat gcatgttaca taataaatca aggagtcctt atgggcgctt ttacaggtaa
    41281 gacagttctc atcctcggtg gcagtcgtgg tatcggtgcc gctatcgtac gtcgtttcgt
    41341 caccgatggg gccaatgtac gattcaccta tgcggggtcg aaagatgccg ctaaacgcct
    41401 ggcacaagag actggagcga cagcagtatt cacagatagt gctgacagag acgctgtcat
    41461 tgatgtcgtt cgtaagagcg gcgcattgga tatcctggtg gtaaatgcag gtattggcgt
    41521 ctttggcgag gccctggaat taaatgccga cgatattgat cgccttttca aaatcaatat
    41581 tcatgctcct tatcatgcct ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat
    41641 cttaatcatc ggctccgtga atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc
    41701 cgccagcaaa tctgccctgc aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg
    41761 tgggatcacc attaacgtcg tccagccagg gccaattgat accgacgcta atcccgccaa
    41821 cgggccaatg cgcgatatgt tgcatagttt gatggctatc aaaagacatg ggcaaccgga
    41881 agaggtcgct ggtatggtcg catggttagc agggccagaa gccagttttg ttaccggcgc
    41941 gatgcatacc attgatggcg cgtttggcgc ataaccgact acgctcaatt aagcccagcc
    42001 attatccatg atgtctgggt tttgtttact cacgtcgtcc gctaaaagcg gctcccggta
    42061 aagagaaatc ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta gaaagaaccg
    42121 ctgttttccc tgctggccga cattcactat atgctgagca tcgttattct gcggctattc
    42181 gttccggcga tttgctgttt gtttccgggc aagtaggaag tcgagaggac ggaacaccag
    42241 aacccgattt tcagcaacaa gtcagactgg catttgataa tttgcatgcg accttggcag
    42301 ctgcgggatg cacttttgac gatatcattg atgttacgag cttccatacc gatccagaaa
    42361 accaatttga agacatcatg acggtgaaaa atgaaatatt tagcgcccca ccttatccaa
    42421 actggacggc ggtgggtgtt acatggctgg caggctttga ttttgaaatt aaagtgatag
    42481 cgcgcatccc tgagcagtaa gcaatagtgt tagccgttcg ctttcacact ccgccctata
    42541 agtcggatga atggaataaa atgcatatct gattgcgtga aagtgaaaaa gaaaaaagca
    42601 gggaatgtct gcaattattg ataccgaacg acagttcccc tgcagaatca catcaaataa
    42661 aaatgcatat accttgactt ttaattcaaa taaaccgttt gcgctgacaa aatattgcat
    42721 caaatgcttg cgccgcttct gacgatgagt ataatgccgg acaatttgcc gggaggatgt
    42781 atggttcagt gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc tggcctgccg
    42841 tttttcttcc cgttgatcac ccattcccag cccctcaatc gaggggcttt tttttgccca
    42901 ggcgtcagga gataaaagat ggctaatccg ctatatcaga aacatatcat ttccataaac
    42961 gaccttagtc gcgatgacct taatctggtg ctggcgacag cggcgaaact gaaagcaaac
    43021 ccgcaaccag agctgttgaa gcacaaagtc attgccagct gtttcttcga agcctctacc
    43081 cgtacccgcc tctctttcga aacatctatg caccgcctgg gtgccagcgt ggtgggcttc
    43141 tccgacagcg ccaatacatc actgggtaaa aagggcgaaa cgctggccga taccatttcg
    43201 gttatcagca cttacgtcga tgcgatagtg atgcgtcatc cgcaggaagg tgcggcgcgc
    43261 ctggccaccg agttttccgg caatgtaccg gtactgaatg ccggtgatgg ctccaaccaa
    43321 catccgacgc aaaccttgct ggacttattc actattcagg aaacccaggg gcgtctggac
    43381 aatctccacg tcgcaatggt tggtgacctg aaatatggcc gcaccgttca ctccctgact
    43441 caggcgttag cgaagttcga cggcaaccgt ttttacttca tcgcgccgga cgcgctggca
    43501 atgccgcaat acattctgga tatgctcgat gaaaaaggga tcgcatggag tctgcacagc
    43561 tctattgaag aagtgatggc ggaagtagac atcctgtaca tgacccgcgt gcaaaaagag
    43621 cgtctggacc cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg cgccagcgat
    43681 ctccacaacg ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt tgatgagatt
    43741 gcgacggatg ttgataaaac gccacacgcc tggtacttcc agcaggcagg caacgggatt
    43801 ttcgctcgcc aggcgttact ggcactggtt ctgaatcgcg atctggtact gtaaggggaa
    43861 atagagatga cacacgataa taaattgcag gttgaagcta ttaaacgcgg cacggtaatt
    43921 gaccatatcc ccgcccagat cggttttaag ctgttgagtc tgttcaagct gaccgaaacg
    43981 gatcagcgca tcaccatcgg tctgaacttg ccttctggcg agatgggccg caaagatctg
    44041 atcaaaatcg aaaatacctt tttgagtgaa gatcaggtag atcaactggc attgtatgcg
    44101 ccgcaagcca cggttaaccg tatcgacaac tatgaagtgg tgggtaaatc gcgccccagt
    44161 ctgccagagc gcatcgacaa tgtgctggtc tgcccgaaca gcaactgtat cagccatgcc
    44221 gaaccggttt cttccagctt tgccgtgcga aaacgcgcca atgacatcgc gctcaaatgc
    44281 aaatactgtg aaaaagagtt ttcccataat gtggtgctgg ccaattaatt gcggttggta
    44341 ataaaagtct ggctccctat aatgagccag actttttacc gctgtaataa aggagaaatc
    44401 atgagcaaaa ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta cgtacagggc
    44461 gttgatctgg gcaatatgat catcacctcc ggtcaaatcc cggtaaatcc gaaaacgggc
    44521 gaagtaccgg cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt aaaagcgatc
    44581 gtcgaagccg caggcctgaa agtgggcgac atcgttaaaa ccaccgtgtt tgtgaaagat
    44641 ctgaacgact tcgcaaccgt taatgctacc tacgaagcct tcttcaccga acacaatgcc
    44701 accttcccgg cacgttcttg cgttgaagtt gcccgtctgc cgaaagacgt gaagattgag
    44761 atcgaagcga tcgctgttcg tcgctaatat ctactgaaag ataatccagg tattgcgcct
    44821 gggttatctg attcaaccac ggagggttga agatggctaa accccgtttc actaacgaac
    44881 aaattgctga aattttacag cagtcaaaag agggggcgtc taataaggag ctatgtgaac
    44941 actatcaatt tagcgttagc acgctgcggc gctggcaaga acagcacgct gacggcatca
    45001 gaagcgaatt aaaaaaaaca gaatccaaag cacaaatcgt ctttctggtt ttctttgcta
    45061 tcgctattct actgactctt atatttgaca aacctacagg tggatgggta attccgcctt
    45121 tattaatcta ttgtgtttat tatatccgcc agtatcgcaa catctctggc agacatatta
    45181 aaaaagaaga tatctatctc tctcgttcag taaataacag ctatagcgcc ctttataatt
    45241 tgagctggac gtttatttgt ttttttattt ttgcagtaat ttattttttt attcaggtgt
    45301 tttcgtaaaa gactgacctg aaagagtatt tcaggccagc ctcatcactt gcttactgcc
    45361 agccgtaacg acggctataa aatcctttca ccaactgggt taacgtcata taccctgcca
    45421 aaatcgcaac cagccacggg aaatagctta acggcagcgc ctgcagctgc agataactgg
    45481 ccagcggtga aaacggcaat gcgatcccga caatcatcac gatcacggtc atgatcatta
    45541 acggccacga tgcacagctc tgaataaacg gcacacggcg ggtgcggatc atatgcacaa
    45601 tcagcgtttg cgacagtaag cccaccacaa accatcccga ctggaacagc gtttgcgttt
    45661 ccggcgtgtt ggcatggaat acccaccaca tcaggcaaaa cgtcagaata tcgaagatcg
    45721 agctgatcgg tccgaagaag atcataaagc gccccagatc cgccggattc caacgctgcg
    45781 gcttttgaat ttgctcgtcg tcgacgttat caaacgggat cgccacctgt gacacatcat
    45841 acagcaggtt ctgaataagc aagtgtaacg gcaacatcgg caggaagggc aagaaagcac
    45901 tcgccaccag cacgctgaac acattaccga agttggagct cgccgtcatt ttgatgtatt
    45961 tcagcatgtt ggcgaaagtg cgacgtcctt caataacccc ctcttccagc accatcaggc
    46021 ttttttccag caggatgata tcagccgctt cacgggcaat atctaccgcg ccgtccacag
    46081 aaatgccgat atccgcagcg cgtaaggcgg gcgcatcatt aataccatcg cccataaagc
    46141 caaccacatg cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc atcggcgtca
    46201 ggcgggcaaa cagcgtggtg cgctgtgcga gatttgccag ttcgtcgtca gatagcgttt
    46261 caatatcact accaatgacc acctctcccg cgtccagccc cacttcatgg cacactttcg
    46321 ctgcaactaa ctcactgtcg ccagtaagga tttttacggt gattccactc gcttttaatg
    46381 ccttcagtgc cggagcggtt gtctcttttg gcggatcaag gaaagcaata tatccttcga
    46441 ggatcaggtc ggattcatcc gcccgctggt aatctccttc acgcgctggc aggtatttcg
    46501 tcgccaccgc aaccacgcgc agcccctgac gattaagcgt atcagtaacc cgcttaatct
    46561 tacgcagcat gatgtcatcg agcggcacaa tctcgccatt gtgacgcacc tgcgaacaca
    46621 cattgaggat ttcctgcaat gcacctttgc aaaccagctg atggtgctcg gtattttctg
    46681 ccactaccac cgacatccgg cgacgctcga aatcaaacgg aatctcatca attttttgcc
    46741 aacgactggc cagcgagcgc gctgactctt catccgtacc ttcgagcacc gctgtatcga
    46801 gcaggttttt aagtccggtc tgataatgac tgttcaacca cgcgctatgc agcacgcgtt
    46861 cgctggtttt accggagata tcggtatgat tctccagcac aattttgtcc tgtgtcaggg
    46921 tgccagtttt atcagtgcac agaatatcca ttgcgccaaa gttctgaata gcatccagat
    46981 gtttgacgat cactttctgt ttcgacagct ttactgcgcc gcgcgccagc gtcgaggtaa
    47041 caatcatcgg caacatttcc ggcgttaagc ctaccgcgac cgaaagcgca aacagtgccg
    47101 cttcccacca gtcgcctttg gtgtaaccat tgattaacag caccaccgga gccatcacca
    47161 gcataaagcg aatcagcagc atactgacgc ggctgatccc ttgctgaaag gcattcggct
    47221 cgctttcctg ctcactaaca cgccccgcca gttgaccaaa ccaggtattg gcacctgtag
    47281 caatcaccat tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa cacagggtgt
    47341 cacactccag cggattgctg tgctccggct ggcgagtggt agcggctttt tctacgggca
    47401 gagactcacc ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc aagatacgta
    47461 aatctgccgg gatcatatcc cccgccgcca gttttattat atcgccgggc accagctggt
    47521 cgatcgggat ctccagccag ccattttcgc ctttgtcgtt aattacgcgc agaaccgtcg
    47581 ctgtattgct gaccatcgct ttcagggcat ctgccgcttt agtggaacgt gcttcctgaa
    47641 taaagttcag caacgtagaa atagcgacca ttagcgcgat aacgcccgcg gcaaataaat
    47701 cttccgtggc gtaagaaata gcgccgagaa tggtgagtaa aatattaaag gggttgcgat
    47761 agcagaccca taaatgtacc caccacggcg acggttgttg tgcgggtaat ttattttcac
    47821 catgttgttc gcgggcagat tccacttccg cctgatttaa cccttccgga tgggtgtcga
    47881 acgttttcca taattcttct tcgggcatca ccgccatttt caggcaatgc gcacttaagg
    47941 acggcgggac cgccgtattc actgtctgct gcgcgccagg caatggatcg cgatgaacca
    48001 gacgggaagg taaatggcga atgagccggg taaaaatttc tttaaacata aggagtccct
    48061 ccgcactgtc tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt acctgaaggt
    48121 aataaagctg agcagggacg ttaaaacgtc acggccttac gcatccggta acgccgtgaa
    48181 aagacgggct taccggaaaa gttttaatct ccgtcgaggg agaggcgtgg gatcaggttc
    48241 catataacct ccggtaagtg aaaaaagcgc cgcaaataat aaggagaatc caataatatt
    48301 cgtagcatct atggcgatat aatacctgct ggcaactaaa ccaacgataa accagacttt
    48361 acccattgct gaatgcacgg gtaaagttag gctcaaataa ttaaacaaca cgttacagga
    48421 caacaggatg caaaatcggc tgaccatcaa agacatcgca cgcttaagcg gcgtggggaa
    48481 atctacagtt tcccgggtgc tgaataacga aagcggcgtg agccagcgca cccgcgagcg
    48541 tgttgaagca gtgatgaatc agcatggatt ttccccttcc cgctctgcgc gcgctatgcg
    48601 tgggcaaagc gataaagtgg tcgccatcat tgttacccgc ctggattcgt tgtcagaaaa
    48661 tctcgccgtt caaaccatgc tgccagcgtt ctatgaacaa ggttacgacc caatcatgat
    48721 ggaaagtcag ttttccccgc aattagttgc cgaacatttg ggggtgctga aacggcgtaa
    48781 tatcgacggc gtagtgctgt tcggttttac tggcataaca gaagaaatgt tagcccactg
    48841 gcagtcatcg ctggttctgc tggcgcgtga cgcaaaaggt tttgcttcgg tctgttatga
    48901 cgacgaaggg gcaatcaaga tcctgatgca acggctgtat gaccaggggc atcgtaatat
    48961 cagttatctc ggcgtgccgc atagtgacgt gacgaccggg aagcgacgtc acgaagccta
    49021 cctggcgttc tgcaaagcgc ataaactgaa tcccgttgcc gctctgccag gccttgctat
    49081 gaagcaaggc tatgagaacg tagcaaaagt gattacgcct gaaactaccg ccttactgtg
    49141 cgcaaccgac acgctggcac ttggcgcaag taaatacctg caagagcaac gcatcgacac
    49201 cttgcaactg gcgagcgtcg gtaatacgcc attaatgaaa ttccttcatc cggagatcgt
    49261 caccgtagat cctggttacg ccgaagctgg acgccaggcg gcctgccaat tgatcgcaca
    49321 ggtaaccggg cgcagcgaac cgcaacaaat catcatcccc gccaccctgt cctgatcgtt
    49381 tcctgaacga taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt tttaaatttt
    49441 tccgcgcaat atattctgca gccaaccaaa aatgtcatct gccatggggc tttatgatga
    49501 gcaaaataaa ccaaacggat atcgatcggt tgattgaact ggtcggcggg cgcggcaata
    49561 ttgcgacggt gagccactgt attactcgcc tgcgctttgt cctcaaccaa ccggccaatg
    49621 ccagaccgaa agaaattgag caactcccca tggtgaaagg ctgtttcacc aatgccgggc
    49681 aatttcaggt ggtgattggc accaacgtgg gtgattacta tcaagcacta atagcgtcaa
    49741 ccggacaggc gcaggttgat aaagagcagg taaaaaaagc cgcccggcag aatatgaaat
    49801 ggcatgagca gttgatctct catttcgcgg agatcttctt cccgttgctg cccgcgttga
    49861 ttagcggcgg tttgatcctc ggttttcgta atgtgatcgg cgatttgccc atgagcaacg
    49921 gtcaaacgct ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt ctgtggttga
    49981 tcggtgaagc gatcttcttc tacctgccgg tcgggatttg ctggtcagcg gtgaaaaaaa
    50041 tgggcggcac gccgatcctt ggtatcgtgc ttggcgtgac actggtttcc ccccagctga
    50101 tgaacgctta tctgctcggg cagcagctgc cggaagtgtg ggactttggc atgttcagca
    50161 tcgccaaagt gggctatcag gcgcaggtga tcccggcact gttagccggg ctggcgctgg
    50221 gcgttattga aactcgcctt aaacgcatcg taccggatta cctctatctg gtggtggtgc
    50281 ccgtctgttc gctgatcctc gcggtgttcc tcgcccatgc gctgattggt ccgtttggtc
    50341 gcatgattgg cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc agctttgctc
    50401 cgattggtgc ggcattgttt ggcttcctgt acgcgccgct ggtgatcacc ggcgtacacc
    50461 agaccaccct tgctattgat ttgcagatga ttcaaagcat gggtggcacg ccagtgtggc
    50521 cgctgattgc gctgtcgaat atcgctcagg gctccgccgt gataggcatt atcatttcca
    50581 gccgcaagca caatgaacgc gagatctccg tgcctgccgc tatctccgcc tggcttgggg
    50641 tcactgagcc tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg ctgtgcgcga
    50701 tgattggttc tggtctggca ggattactat gcggcctgaa cggcgttatg gcgaatggta
    50761 tcggcgtagg cggcctgccg ggaattctct cgattcaacc gagctactgg caggtatttg
    50821 cgctggcaat ggttatcgcc atcatcatcc cgattgtact cacctcgttt atctatcagc
    50881 ggaaataccg cctgggcacg ctggatattg tttaattttc ttcggggcgc aattgcgccc
    50941 cctcgcattt gcaggaataa cgtaatgact aatcttcccc actggtggca aaacggcgtt
    51001 atctaccaga tttatccaaa gagttttcag gacaccacgg gtagcggcac cggcgattta
    51061 cgtggcgtta tccaacgcct ggactatctg cataaactgg gcgttgatgc catctggcta
    51121 accccctttt atatctctcc ccaggtcgat aacggttacg acgtagcgaa ctatacggcg
    51181 attgatccca cctacggcac gctggacgat tttgacgaac tggtgacgca ggcaaaatcg
    51241 cgcgggattc gtatcattct cgatatggtg tttaaccata cctctaccca acatgcctgg
    51301 tttcgcgagg cgctgaacaa agaaagccct taccgccagt tttatatctg gcgcgatggc
    51361 gaaccagaaa cgccaccgaa caactggcgt tcaaaatttg gcggtagtgc gtggcgctgg
    51421 catgcggaaa gcgaacagta ctatttgcat ctctttgcac cagaacaggc ggatctcaac
    51481 tgggagaatc cagcggtacg cgcagagctg aaaaaagtct gtgaattctg ggccgatcgt
    51541 ggggtcgacg ggttgcgcct ggatgtggtg aatctgatct ccaaagaccc gcgtttccct
    51601 gaagacctgg atggcgacgg gcgtcgcttt tacaccgacg ggccacgagc acacgagttt
    51661 ttgcacgaga tgaaccgcga tgtgtttacg ccacgcgggt taatgaccgt aggtgaaatg
    51721 tcctccacca gccttgagca ttgccagcga tacgcggcac tgacaggcag tgaattgtcg
    51781 atgaccttta attttcatca cctgaaggtc gattatcccg gtggtgaaaa atggacgctg
    51841 gctaaacctg actttgtggc gttgaaaaca ttgttccgcc actggcaaca aggaatgcac
    51901 aacgtggcat ggaatgcctt gttctggtgt aaccacgatc agccgcgcat tatttctcgc
    51961 tttggtgatg aaggtgaata ccgcgtgcct gcggcaaaaa tgctggcgat ggtgctgcat
    52021 ggcatgcagg gaacgccgta tatctaccag ggcgaagaga ttggcatgac caacccgcat
    52081 ttcacgcgca ttactgacta tcgcgacgtg gagagcctca atatgtttgc cgagctgcgc
    52141 aacgatggtc gtgatgccga cgagttattg gcaatcctcg ccagtaaatc ccgtgacaac
    52201 agccgcacgc ccatgcaatg gagcaacggc gataatgccg ggtttacggc tggcgaaccg
    52261 tggattggcc tgggcgataa ctatcaacaa atcaacgtag aagccgcgct ggccgatgac
    52321 tcctcggtgt tttacaccta ccaaaagtta atcgcactgc gtaagcaaga agccatcctg
    52381 acatggggca attaccagga tctgctgcca accagccctg tattgtggtg ctatcgccgt
    52441 gaatggaagg ggcaaacctt gctggtcatt gccaacctta gccgtgagat ccaaccctgg
    52501 cagccagggc aaatgcgcgg caactggcag cttgtgatgc ataactacga agaagcctca
    52561 ccacaaccct gtgccatgac tttacggcct tttgaggctg tctggtggtt acagaagtaa
    52621 gtatccgcta agccccggga atgcctgtca ttccggggtt ttgtctgtca gcatgttgtt
    52681 tttgttgatt tttcaaccag caaattcatt aaaaaattta cataacgctg tagcgcccgt
    52741 catccgtacg ctctgctttt tactttgagc tacatcaaaa aaagttcaaa catccttgat
    52801 gcaaagcact atatatagac tttaaaatgc gtcccaaccc aatatgttgt attaatcgac
    52861 tataattgct actacagctc cccacgaaaa aggtgcggcg ttgtggataa gcggatggcg
    52921 attgcggaaa gcaccggaaa acgaaacgaa aaaaccggaa aacgcctttc ccaatttctg
    52981 tggataacct gttcttaaaa atatggagcg atcatgacac cgcatgtgat gaaacgagac
    53041 ggctgcaaag tgccgtttaa atcagagcgc atcaaagaag cgattctgcg tgcagctaaa
    53101 gcagcggaag tcgatgatgc cgattattgc gccactgttg ccgcggttgt cagcgagcag
    53161 atgcagggcc gcaaccaggt ggatatcaac gagatccaga ccgcagttga aaaccagctg
    53221 atgtcgggtc catacaaaca actggctcgt gcttacatcg agtaccgtca cgatcgcgac
    53281 attgaacgtg aaaaacgcgg tcgcctgaac caggagatcc gtggtctggt cgagcagacc
    53341 aacgcctcgt tactcaacga aaacgccaac aaagacagca aggtgattcc aacccagcgc
    53401 gacctgctgg ccgggatcgt ggctaaacac tatgcacgcc agcacctgct gccgcgtgac
    53461 gtggtgcagg cacatgagcg tggcgatatt cactatcacg atctcgatta ctcgccgttc
    53521 ttcccgatgt tcaactgcat gttgatcgac ctgaaaggca tgctgaccca gggctttaaa
    53581 atggggaacg ccgagattga accgccgaag tcgatctcta cggcaaccgc ggtaactgcg
    53641 cagattattg ctcaggtcgc cagccatatt tatggcggca ccaccattaa ccgtatcgat
    53701 gaagtgctgg cgccgtttgt taccgccagc tacaacaaac atcgcaagac cgcagaagag
    53761 tggaacatcc cggacgccga aggctacgca aactctcgta ccatcaaaga gtgctacgat
    53821 gccttccagt cgctggagta cgaagtaaac accctgcaca ccgccaacgg tcagacgccg
    53881 tttgtaacct ttggttttgg tctgggcacc agctgggaat cgcgcctgat tcaggaatcg
    53941 atcctgcgta accgtatcgc aggcctcggt aaaaaccgta aaactgcggt gttcccgaaa
    54001 ctggtgtttg cgattcgcga tggcctgaac cataaaaaag gcgatccgaa ctacgacatc
    54061 aaacagctgg cgctggagtg cgcaagcaag cgcatgtatc cggatatcct gaactacgat
    54121 caggtagtga aagtcaccgg ttcgtttaaa accccgatgg gctgccgcag cttcctcggc
    54181 gtgtgggaaa atgaaaacgg cgagcagatc cacgatggtc gtaacaacct cggcgtgatc
    54241 agcctgaacc tgccgcgtat tgctctggaa gcaaaaggcg atgaagccac cttctggaag
    54301 ctgctggatg aacgtctggt gctggcacgt aaggcgctga tgacccgtat cgctcgtctc
    54361 gaaggcgtga aagcgcgcgt ggccccgatc ctctatatgg aaggtgcttg tggcgtgcgt
    54421 ctgaatgctg acgatgatgt ttctgaaatc ttcaaaaacg gtcgtgcgtc tatttcgctg
    54481 ggttacatcg gcatccacga aaccattaac gcgctgttcg gcggcgagca tgtatatgac
    54541 aacgagcagc ttcgcgcgaa aggtatcgcc attgttgaac gtctgcgtca ggcagtggat
    54601 cagtggaaag aagaaacggg ttatggtttc agtctctaca gcacgccgag tgaaaacctg
    54661 tgcgaccgct tctgccgtct cgatactgct gagtttggcg tggtgccggg cgtgaccgat
    54721 aaaggttact acaccaacag tttccacctc gatgtggaga agaaggtgaa cccgtacgac
    54781 aagatcgact ttgaagcgcc ttacccgccg ctggcgaacg gtggtttcat ttgctacggc
    54841 gagtatccaa acattcagca caacctgaag gcgctggaag atgtctggga ttacagctat
    54901 cagcatgtac cgtattacgg caccaataca ccgattgatg agtgctacga gtgtggcttt
    54961 accggtgagt tcgagtgcac cagcaaaggc ttcacttgcc cgaaatgtgg taaccatgac
    55021 gcctcccgtg tgtcggtaac tcgccgcgtg tgcggatatt taggtagccc ggatgcacgt
    55081 ccgtttaacg ctggtaagca ggaagaagtt aagcgccgcg ttaaacattt ggggaatggg
    55141 cagataggtt aatcttcact ctgcgctaag caaatgccgg atgcggcgta aacgccttat
    55201 ccggcctaca tttggcaacg gctgtaggcc tgataagacg cgacagcgtc gcatcaggca
    55261 ttgattaccg gatgcagcct acacgcggac tgataagcca ggcgaaataa tgaattatca
    55321 tcagtactat cctgtcgaca tcgtcaacgg ccccggcact cgctgcaccc tgtttgtctc
    55381 cgggtgtgtt catgaatgcc ccggttgcta taacaaaagc acatggcggg taaattctgg
    55441 tcagctattt accaaagcaa tggaagacca gatcattaac gatctgaatg acactcgtat
    55501 caaacgccag ggtatttccc tctccggcgg cgatccgctg catccgcaaa acgtgccgga
    55561 tattctgaaa ctggtacaac gcatccgcgc cgagtgtccg ggtaaagaca tctgggtgtg
    55621 gacaggctat aaactcgacg aactcaacgc tacgcaaatg caggttgttg atttgattaa
    55681 cgtgctggtc gacggcaagt ttgtgcagga tttaaaagac ccttccctga tctggcgcgg
    55741 cagcagcaat caggtggtgc atcatttgcg atgataagtt ccctgttacc tgagtgcagg
    55801 caacagggaa atgaggaatt aacgatactt ctggtgatag gcgttgcggg tcgttttcag
    55861 ttgctctgcg gcggcctgcg cttcttttac tttaccttca tttgccagct tcagcgcgtc
    55921 gtcaatctga ccgaccagaa tgtcgaaacc gtggcggaaa tctttcattt ccgggctgtc
    55981 cggtgattta tcttcgagct tcggcggcgt tgctttttgc gcatccagcg ctgcggcgcg
    56041 catcatcgtt aacgcgtctt tgacttgcgc cgcgttatcc gctttttcga tcacttttaa
    56101 attgtcgttg agggtttcca tattgtcttc aagatcagcg gcaaacgacg cagaactgaa
    56161 taccaacgag gagactgcaa gaatagctaa caggctttta cgcattgctc acttcctttt
    56221 ttatttacac ttcatccttc acactgcctc atcgacggct acgctcattc gcccgggtca
    56281 cgtactgcga gtacgctccc cggccctcat tcactttccg tctcgatgca gtgcgaatga
    56341 ttttgtgtaa ttttttagtt tatagcgcgg caggtcgcgc cagtttttta ctgcccggcg
    56401 attttcatct ccggcaacag cacagaacca cactgtatat tactgcgtgt ttcaatatcg
    56461 ttaccgacgg tgacaatatt gcgccacata tcttttaaat tacctgcgat ggtgatttca
    56521 ctaaccggat actgaatttc gccattctct acccagaagc ccgcagcacc acgggaataa
    56581 tcaccagtaa tggcactcac gccctggccc atcagctccg tcaccaccag cccggtgccc
    56641 atctctttga gcatttgttc gaagcttagc ccttgtccgg caatccgcca gttgtgaata
    56701 ccgcccgcat gtccggtgct tttcagcccc agtttccgcg ccgagtagct ggtcagcagc
    56761 cactgagtca ggatgccatc tttaataata tcgcgacgct cggtgcgcac accttcgctg
    56821 tcgaatggcg tcgacgccag ccctttcagc agatgcggat gctcttcaat ggtcagccag
    56881 tccggcagaa tttgtttacc cagcgaatcc agcagaaagg tagatttacg gtaaaccgag
    56941 ccacccgcta tcgcccccac cagatggcca aaaagcccgg ttgccacttc attggcaaaa
    57001 atgactggcg ctttcatggt ggagagttta cgcggtgaca ggcgcgataa agtacggcga
    57061 gcacagtcgg ccccaaccca ctctggcgtt tgcagatcgc tcatcgcacg accaatggtg
    57121 taggcgtaat cacgctccat atcgccattt tcttcggcaa ttacacagct ggagagtgaa
    57181 tgacgcgtag agcagtaacc ctgcaacatg ccgtggctgt tgccaaaaac tttgacaccg
    57241 tagtggctgt taaaactgcc gccttcggta ttggtaatgc gtttgtccgc ctgcaatgcc
    57301 gcctgttctg cgcgggccgc cagttcaatg gcatcatccg gggaaacttc agcagggtgg
    57361 aacaagtcga gatctggtgc gtcaaaggcc agcagctctt tgtctgccag gccggcacag
    57421 ggatctggcg aggtataacg ggcaatatcc agcgccgcct gtacagtacg ggcaatggcc
    57481 tgcgggctta aatcggtgga tgatgcgcta cctttgcggt tctggtgata aacagtgatc
    57541 cccagcgcgc catcgctatt gaattcaaca ttctccactt caccataacg cgtgcttacg
    57601 ctaatgccgg tggtcttgct gacggcaact tccgcaccgt ccgatttgcc tgaggccaac
    57661 tccagcgcag tggaaactgc ttcttccaga atcttgcgct gcgcttcaac ttgagagatt
    57721 actttcattg caagtgccat aatgtagaga gtttctctga agtctaacag agaaccgttt
    57781 ttcagtgcgc atcttaactg gtaacattag cctctttttt taaggagcct gagatgacta
    57841 agcagcccga agactggctc gacgacgttc ccggtgatga catcgaagac gaagacgatg
    57901 aaattatctg ggtcagtaaa agtgaaatta aacgtgatgc cgaggagcta aaacgccttg
    57961 gcgcggaaat tgttgatctg gggaaaaacg cgctggataa gatcccgtta gatgcggatc
    58021 tgcgcgcggc tattgagctc gcccagcgta ttaagatgga aggtcgccgc cgccagttgc
    58081 agctcatcgg taagatgctg cgccagcgcg acgtagagcc tattcgtcag gcgctggata
    58141 agctgaaaaa tcgccacaat cagcaggtgg tgctgttcca taaactggaa aacctgcgcg
    58201 atcgtctgat cgatcaaggt gatgacgcca tcgctgaagt attgaatctg tggcccgatg
    58261 ccgatcgtca gcagctgcgt actctgatcc gtaacgcgaa gaaagagaaa gaagggaata
    58321 agccgccgaa atccgcacgc cagattttcc agtatctgcg cgaactggcg gagaatgaag
    58381 ggtaattttc gttgttgatg tgagtatggt ttgccggatg cggtgcacct gtaggcctga
    58441 taagacgcat caagcgtcgc atcaggcact atgctcaaat gccggatgcg gcgtgaacgc
    58501 cttatccggc ctacgcacac tatatcaggc tgaggccgaa ttactgcgcg gcttccgcct
    58561 tcttcgccag accatccagc agtttctgat ggatgccacc aaaaccgccg ttgctcatga
    58621 ccagaatatg gtcgccaggc tgagcggttt tcaccaccat atctgccagc gtatccacat
    58681 cgccactcca gtgtgcaggc tgaacgcagg cttctgccac ttctgccacc tgccacggaa
    58741 tatgcgccgg ttgcagcagg aagacttcat cggcacgacc taatgaaggt gccagatcgt
    58801 ctttgcagat ccccattttc atggtatttg agcgcggttc cagcacagca ataatgcgcg
    58861 ccgtaccacc aactttgcca cgcagcgccg ccagcgttgc cagaatcgcc gtcgggtgat
    58921 gggcaaaatc gtcatatacc gtcacgccat tcgcttcacc acgcaactcc agacggcggc
    58981 gagcgttaat aaacgaaccc agcgcgttag cggcatctgc aggcgctaca ccaacatggc
    59041 gagccgctgc aatcgccatc aggccattgt gcatattatg ttcgcctacc agcgaccatt
    59101 tcacttcgcc cactttttcg ccatcaagca agacttccca ttcggaagca tcggtggtca
    59161 gctttttcgc ctgccagtgc ccctgctcac ccaccagctc ctgctcgctc cagcagccca
    59221 tcgccatggt ctgtttcagg ttgatgtcat tttccggcca gataatacgg ccctgccccg
    59281 gaacgatacg caccagatgg tggaactgtt tctggatcgc tttcaggtcg tcaaagatat
    59341 cggcgtgatc gaactcaagg ttgttgagga tcagcgtacg cgggcagtaa tggacaaatt
    59401 tagagcgttt gtcgaagaag gcgcagtcgt actcatccgc ttcgataaca aagaagtcgc
    59461 tttcgcccag acgcgccgaa acctcaaagt tccccggcac accgccgatt acaaagcccg
    59521 gtttgtaacc acactgttcc agaatccagg tcgccattcc cgcggtggtg gttttgccgt
    59581 gtgtacctgc aacggccagc acccagcggt cgcgcagcac aaaatcgtgc agccactgtg
    59641 gacctgacat ataagggatg tttttttcca gtaccgcttc cacacacgga tttccacggg
    59701 tcatggcgtt gccaataatc accagatccg gctgcggatc gagctggctg gcatcgtaac
    59761 cctgaatcag ttcaatgcct tgcttctcaa gtaaggtgct catcggcgga tacacattgg
    59821 cgtccgaacc cgttacttca tggcctaact ggcgcgccag catcgccaga ccgcccataa
    59881 acgtgccaca aattcctaaa atatgaatgc gcatacgtca ctatcctttt ttaatctgcc
    59941 gctcatttta cgcatatgtc gggcaagtga gaaacgcatt tcaggataat ccgcaatttg
    60001 ctggcgcgat tcacctgagc gcaacattcc gattatttgt taagattgtt gcggtcgctt
    60061 tactccataa acattgcagg gaaagtttta tgaaaacgtt aggtgaattt attgtcgaaa
    60121 agcagcacga gttttctcat gctaccggtg agctcactgc tttgctgtcg gcaataaaac
    60181 tgggcgccaa gattatccat cgcgatatca acaaagcagg actggttgat atcctgggtg
    60241 ccagcggtgc tgagaacgtg cagggcgagg ttcagcaaaa actcgacttg ttcgctaatg
    60301 aaaaactgaa agccgcactg aaagcacgcg atatcgttgc gggcattgcc tctgaagaag
    60361 aagatgagat tgtcgtcttt gaaggctgtg aacatgcaaa atacgtggtg ctgatggacc
    60421 cactggatgg ctcgtccaac atcgatgtta acgtctctgt cggtaccatt ttctccatct
    60481 accgccgcgt tacgcccgtc ggcacgccgg taacggaaga agatttcctc cagcctggta
    60541 acaaacaggt tgcggcaggt tacgtggtat acggctcctc taccatgctg gtttacacca
    60601 ccggatgcgg tgttcacgcc tttacttacg atccttcgct cggcgttttc tgcctgtgcc
    60661 aggaacggat gcgcttcccg gagaaaggca aaacctactc catcaacgaa ggaaactaca
    60721 ttaagtttcc gaacggggtg aaaaagtaca ttaaattctg ccaggaagaa gataagtcca
    60781 ccaaccgccc gtatacctca cgttatatcg gttcactggt cgcggatttc caccgtaacc
    60841 tgctgaaagg cggtatttat ctctacccaa gcaccgccag ccacccggac ggtaaactgc
    60901 gtttactgta tgagtgcaac ccgatggcat tcctggcgga acaagcgggc ggtaaagcga
    60961 gcgatggcaa agagcgtatt ctggatatca tcccggaaac cctgcaccag cgccgttcat
    61021 tctttgtcgg caacgaccat atggttgaag atgtcgaacg ctttatccgt gaattcccgg
    61081 acgcgtaatc tcctgcgcct ctccacacgg agaggcgttt ttatcgctgc gcaatgttga
    61141 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaatcca cgctgtaatg
    61201 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccaccatgag ctcagcgtgc
    61261 catcaaagtt tatgtaagtc tgaatcagtc cctgaatcgc cacgccaaaa agcgtcccta
    61321 ataccgttcc aacgccaccg ctcaaaagcg taccgccaat taccactgag gcgatagcat
    61381 ccagttccac acctacgccc gccagcgcat atccggcctg ggtataaatc gagaagacaa
    61441 tccccgccag cgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcggg
    61501 tagaaatccc catcaggttt gccgacgttg cgttgccgcc aatggcgtat acctgattac
    61561 caaaacgggt acgatgcgcg aggaatatgc cgataaccac caccgccagc atcagcagtc
    61621 ccatcgcact taagcgaccg ccgccaggga ttttccacgc aaggcttgag agcgtgtcat
    61681 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
    61741 agaacattcc tgccaaagta atgataaacg ccgggatctt cagggcgtcg atcaaaagcc
    61801 ccataaatgc gccaaaggca cagcccatta ccagcaccag cggaaatgcc agcaacggcg
    61861 agaggccgaa gtcgccaatc acttttgcca gaaacacgcc ggtaaaagcg atcaccgaac
    61921 cgacggagag atcgatccca ccggagagga tcacaaaggt catgccaacg gcaatgatcc
    61981 caagaaaggc gttatcggtc aggatattgc agatcactct tgtggaagca aaaccgggaa
    62041 actgcgtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagtggca
    62101 aattacgttt tatcacggct acgtactcct ttaatcagac tgataaagcg ttgcgactgg
    62161 acaatcagca cacaaagcac caccaccgct tttacgacct ggttcatctc cggcggaaag
    62221 ccagaaagta aaattccggt attcattccc tgaataatca gcgcccccac caccgaaagt
    62281 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
    62341 tccagctcca gccataaccc ggcgttattg gcatcggcac cgcgaatatc cgccgccacg
    62401 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
    62461 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacggc ttcgataaac
    62521 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
    62581 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
    62641 ttaaacgtga cgatctggcc ggaggtgatc agttgcgcca cgccgcgccc ggcgaccatc
    62701 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
    62761 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
    62821 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc tacggagaga
    62881 tcaatcccac cggtggcgat caccagcgtc ataccaatcg ccagtaacgc aacgggagct
    62941 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
    63001 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaatagcgc cgccagctgc
    63061 gacattcccg ttggccagcg aaagcgcctt ttcggcggcg tagtgtccgg gagagattga
    63121 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctc
    63181 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg ataatcaccc gatcggcata
    63241 gcccaccagc tcttccagtt cagaggagat caccagcagc gccagaccat cggcgcatag
    63301 cgtttcaatc aggcggatga tctcggcgtg ggcacccaca tcaatgccgc gggttggctc
    63361 atcgaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcaaca ctttttgctg
    63421 attgccgcca gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
    63481 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
    63541 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
    63601 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
    63661 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
    63721 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
    63781 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
    63841 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
    63901 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
    63961 ccgacagcct acgaaactgc cgttgcgtaa gacagtgatc cgatcgctga cctgatagac
    64021 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
    64081 caggccaaat aatagctcca cttcctgggt gtcgaggctg gcggtgggtt catcgaggat
    64141 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgcgac
    64201 tgaaaagcgg ttaagcggtt cgcgcacgtc gaggaagaaa ccgtaagatg ccatcagttc
    64261 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
    64321 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
    64381 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagcct ggccttccag
    64441 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
    64501 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
    64561 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
    64621 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
    64681 taagacgcgg taagcgtcgc atcaggcttc agcgcaccac cgccggatgc ggcgcgagcg
    64741 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
    64801 tcttctctaa ctcttctttt gcggtatcag gcagataaag ggtggatttg gtcagggtca
    64861 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggacctg
    64921 ccatattcgg cgtcagttca acactggcgt tcgcttcgcc atcaatcatc gctttgtaga
    64981 tatccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
    65041 ctttaattgc ctgaattgcg ccaatcacca tgtcatcgtt atgggcgtaa accatgcaga
    65101 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
    65161 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
    65221 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
    65281 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
    65341 cgaggatgtt gtcggcagta acggtggtca tatagagcga tttgtctttc acatcgatgg
    65401 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
    65461 aacctgtcgc gaccaccgga gcgataaaga tcgcatccac cccttgtgca acgaaggaac
    65521 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc agcgtaattc
    65581 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
    65641 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
    65701 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtagt
    65761 tattattggt taaaagatgt tcgcgaaaaa actatagaca atcggtgatg taacgaattg
    65821 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
    65881 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgcat accgtcttaa
    65941 aactggcgaa aaaggaaaat gaagacgaaa acaagcgaag acattcggcg cgagttggct
    66001 ataatactcg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
    66061 cgtccctgcg ggtaaagatc tgccggaaga catctacgtg gttattgaga tcccggctaa
    66121 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
    66181 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
    66241 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc cgggttctgt
    66301 gatccgttgc cgtccggttg gcgttctgaa aatgaccgac gaagccggtg aagatgcgaa
    66361 actgattgct gttccgcaca ccaagctgag caaagaatac gatcacatta aagacgttaa
    66421 cgatctgcca gagctgctga aagcgcagat cgctcacttc ttcgagcact acaaagacct
    66481 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
    66541 aatcgttgcc tccttcgagc gcgcaaagaa taaataagtt cttctggcgc aataaccctg
    66601 aacaccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa cacaccttat
    66661 tccaccaccg cctgcaagcg taataacgcc tcttccacca cctcatccgc agccagacca
    66721 atacgctttg ccagccgggc gcgtagatcc atcatccgca cctgattcac cagcaccacg
    66781 ccgtgcacat cgccttcttc gcaatgtaaa ggaacgctaa atccggcata acgggcaaaa
    66841 tttccgccct gcgtaatggg ggccaccagc gtcattccca gttgattaaa ggcttgaacg
    66901 gagagcacaa gcgcaggtcg accagcacct tgctgttcat ggccgcttgc tggatcaaag
    66961 ccaaccagca caatgtctcc ccgttcaaat tcacttttct ttaccatatt tcgtcacccg
    67021 caggggtgga tttaccccag acatcctgct cgctaagttc cgcggcgttc atgtcacact
    67081 gtgccagcag ttcatcaagc gagtagcgcc tggagatggg tgtcagaatc agttggttgt
    67141 tgctcacctg cgcttccacg ctctgccccg gctgtaagtt aagttctttc attacgatat
    67201 tgggaatgac catacctgca ctgttccccc atctttttat ggtaatacgc atctgcacct
    67261 ccttaagtta tacaaagtat aactcacggt gctacaggaa acgttttacc cctgcacatt
    67321 tgtgaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggct cggagagaaa
    67381 agcaaaagat ggaaaagtta caaaaaaaaa tcgcgagata catcgcataa aacaacgcca
    67441 cccgaaggtg gcgtgctttt attattactt atccctgtct aaccagtcgc cgctttcaat
    67501 tagctttaat ccatcgacgg gtcgttgata aacgtacatc catgcactcc cgtacggcgt
    67561 ctgaatcaac tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa gttcggccag
    67621 cgtggcgttg tcaatacgat aaacttcacc gtgtactgtt ccgttccccg gaactgcgcc
    67681 tggatagtgg cccaggctat acaactggta gttatcgata ctgaaatcgc ccagtaactg
    67741 ggcattggtc atccagtgac tgttgccttg tttgtggcgt aaactgccgt agacaaatat
    67801 tcgcattgct aaaactcgaa ctgatagagc aaatccagtg cctggtctac accagacacg
    67861 gcttccagat atagcttagg catcaggcga taacgtaacg tgagtgttgc tatagagtca
    67921 aatataccca cgccgtattt cacttgcaga cctggcaata catagccgct gaccactacc
    67981 tgggaggagt cgcctactcc ctgggtgtcg agcgctaaat tgcttacgcc aaacgtctcg
    68041 ccgattttac ccacaatctg gccactttgc gcaaccccca ggccaatcag cattgaggtc
    68101 atcgccgcac tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa ataagacaat
    68161 gcagcttgtt gcgacatagc cgggtcagag aagatctccg ctttcggttc gtccgccaga
    68221 ccagtgacgc gaactccggc gattacgtcg tcttctgtag catccgggtt acgaatagct
    68281 tcaatattaa gatacggttg atctggcgga ccagagaaca gtagctcacc cttacgcaca
    68341 atcagatcct gaccataggc atggaagcgc ccttcaggga tgttgatctg cccgttcagg
    68401 cccagccctt gtttgtcctg aacgacgtta agatcgcccg tcagccgcgc tttcaggcca
    68461 aaggcgtcaa tgcgcacatt gttgccaacg tggacaatca ggttactgtt aatcggaatc
    68521 gacgccgttt tcggctcttc cggttgcagg ttatcgttaa gcatcaccac atcgctggag
    68581 acgcctactg cgctttccgg cagatcgtgc accacgatgc gcgcccacgg gacatccacg
    68641 cgaccatcga gggtaaacag gtttggtgta gcctcgaata caacatctgg cgatacatcc
    68701 attcgtacca tcggcggcac ggtgatccgc actttactgc ctttcgccgt tactcgtgcc
    68761 cgccagtttt caatttggct ccagtcggca tcaccgttca ggtagatttc accctgctgg
    68821 gtccgtacgg taccggcaag cgtcgagcgc ataccgttaa agttgaccgc aagctggctc
    68881 ggctgcatat caaacggcat aaagttgccg tcgatatcca cacccgtaac ctgaagctga
    68941 ccaaacaact gcgggctttg cacatcacca cccagacgca agttggcact caccatcccc
    69001 gctgcttttt ccccacgggt aaagatgggg tttatcatcg caaggttgaa gttacggata
    69061 ttgacgttgc caccaagatt acggcggcct tgcggatcgg tcacctgcac ctgtccatca
    69121 aactggccgt tattggtcag gcggatggtc cagcccaatt cggcacggtt gttacgcaat
    69181 tccgccgtca gattcagtgt ctgaaacgcc accggcagcg ccgcatcgtt gacggtttgc
    69241 gttacctgca cgttacgccc cgaaagggtg atactgccct gcggcagccc ctctttcgtg
    69301 gtgtcccagg caacatccgc tttacccgtg aagataccgc tggcctgagt ggtttctggc
    69361 ataaacggtt tcagcatggc gaggtcgaag cggttgagat tcaccaccgc acgcccttcg
    69421 gcccccgcat cgatagtttg cggcacgcac agttccgcat tcgggttaag ccagcagtgt
    69481 ggcccgatgc tgattttttg ctccttattg cggtaatcca gcgcaatatc gcgggtcagc
    69541 gaccacgggc cgaccggcgt ctggaagcgg gtattgctaa gagttccctt ccagcgttct
    69601 tctttgcgat caaaacttcc tgccagatta agctgcccgg aaaccggctc gccctgaatc
    69661 cgcaactgta gctcgtgctg cttttcgctg cctttggcat tcagggtgac gaggttgata
    69721 tttacatccg gctgcgaaat ttgctcaacg cgtacgtcga gtttcccggc gatctgatcg
    69781 gtggatttaa tgtcgccttc cacgcgaacc tgcgtcacgg aaagttcctg ccagcgcagg
    69841 ccgcgcgcgg tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg tactttcacc
    69901 agccctttcg ctgtaccgcc aagccccggc agcgcgttat ccagccccgg cgcgttgatg
    69961 gtggcatcaa gattgagatc ttttaccccc agctcgcctt tcacttcggc actgtttggc
    70021 cccagttcca gatgaagccc tgggatcatc cactgcatat aactgttgcc tttcagcgtg
    70081 ccgtcaacgt tcactttgtt ctgtttgacg ttaccggtca gcttcagttc tggcacctcc
    70141 atctgccagg tgccgccgta caggctaccg cgggttttaa tcaagccatt gagtttcgac
    70201 ggccactccg ggatctcttt ggcggtgtta atgccgttaa gcgttagctc accgcgccaa
    70261 ctaatggcct gctgccagtc gagcaacgcc ttgagttcag ttttcccttc cagcgccgcg
    70321 acggtgagtt tgtcgagatt gacctgctgt tcattacctt tggcatcaag ggtaatggtg
    70381 gctggcggga tctccagtcc cttcactgcc gtacgcatag agagcgtgta atcggtcatt
    70441 ttgccggtaa gtttcagttt cagatcatcc gcctgatact gcttctcacc ggtgaacggc
    70501 cagtaaagct gtttgctgtt cacttccacg ttgagtggca atccggcttc cgccagtcgc
    70561 gtctgggcgc gtaaatccat atccaccgga ccggaaaggt taacgccaat ctccagctgt
    70621 tcgcgcagcg caccgcccac tttcagcttc agtttttctc ctttcaacgg ctccacgttt
    70681 agtgtgcagt tgagggtgat atccaccggc cagttgtctg acagctgcgc cgtgccgctg
    70741 gcgttgacga tcccttgact ggaatcgata tccagggcgt ccagtttagt attgccgtca
    70801 atgctgctca ctttcagcag catggtgcgc acggtgatgt ccgtgtcgcc cgtcacgcgt
    70861 aactgctcgc ctttaaactc ttcaatgttc aggttaagcg gcaaatgcac gtcggtcatt
    70921 tccggcaata ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg cttttcctcc
    70981 ggctgcggat tttcaatttt cggttcgacc acttcttcct gcgccacttc cgccactttc
    71041 ggcagagcaa tcagcaggcc tttcagcgac gtcggtttca gggtcagggt tttctcctgc
    71101 cagttcaggc cggaggtgaa gtccatcacc gacaccgtgg tgtcatcaat cttgatgttg
    71161 acgttgtcca gcgccacccg tgtcagggtg atgggatacg gcgtggagag atccagcgga
    71221 ccgctatctt cctcttcttc aacctgttca gaaggaggca tttttttact gtcgatgttg
    71281 acctgaatgt ctttcagcgc caggtcatta atacaaacgc tactgttcca caggcactca
    71341 agaccgaccg ccagatgcag attacccgct tttaccgcca cgcctggctg ctcataacga
    71401 acgtcagaca aggtgagatc gcgccagccg ccggtgacct tgccaatatc cagtcctggc
    71461 acccagcgat ccgccgcttt aaatacaaga tgcaggccgc tggtggtgcc caccagaaac
    71521 gccaccgatc ccagcaacag taagataacg ataaccacgc cgaggctgat ttttttccat
    71581 aaactcataa ttctggcccc agaccgatgt aaaactgtaa cccgtgttcg tctttatccg
    71641 cgaccggtac ggcaaaatcg agtttgattg gcccgaccgg cgattcccag cgcacgccga
    71701 ccccggtacc ggttttaaag tcgctgcggc gaatatcgct taccgcttcg ccactatcga
    71761 caaacaccgc gccccaccat tttccggtca cgttgtactg gtattccagc gatccggtta
    71821 tcaacttcga ggcccctttc aggtcaccgt tggcgtattt cggggcgata gatttgtatt
    71881 tgtagccgcg aatactgcga tcgcccccgg cgaagaaacg cagatccggc ggtactttgt
    71941 cgaaatcacc ggtttcaatc cagcccagcg tgccgcgtgt aacgaaacga tggcgatcgt
    72001 acagtgtgcg gatccagacg ttctgcgcct ggaaaacgga gaaatcgaca tctgaacccc
    72061 aggccgtgtt ggagtagtcg atagagtagc gttgcgagtc gccccaggtt ggcatcaggc
    72121 caccacgaga acgcgtgcgg ctaatcatca ccccaggata aaacagcatc gtggtgttgg
    72181 taatttcacc ctgggtaaag tggtcgaggc tccagcgcag gttaatggca cgctgccagc
    72241 cgctggagag atcccagtag cgagaagcca ccagcgtagt ggagtcagat tcggtatcgt
    72301 tcaggtcagt gcgcttaaaa ccgccctgca ccaaataata ttgttccagt ggattcttca
    72361 gcagcggcat tttatagctg aagtcgaggg tctgttccgg cgcggaaata ctggtactgg
    72421 tggtcagact gtgaccataa gagttcatcc acggcttttt ccacgtcgct ttcacgcgcg
    72481 gtcccacgtc cgtagagtaa ccgaccccgg tttcgatggt gttttcagtt cgcggcgaaa
    72541 ccacgcccgt caagggtaat actttcgttt cgcgcgcttt atcaaattgt ggagccacca
    72601 ccaccgagtt aaaccagccg gtagcagaaa gtcggcggtt cagttccgcc agatctttcg
    72661 attcgtactc atcgccctct ttaaacggca ccagattttg caggtattca tcgcggattt
    72721 gtgatccttc aaaggtcaca tgcccaaagc ggtaacgttc gccactgtta taatcaatat
    72781 cccagaaggc tttatgcagg ccgagcgcaa tgcccagctg cgctttggta aattcgctat
    72841 cgaaataacc tttacgcaac gcaatgctgg ttaaggactt tttgaaattt tcataatcgc
    72901 cctggttcag caccgtgcca atagccgggc gagtatcgag caatttcaaa tagtctttat
    72961 cggtccgcgc gccgccgcgc aataccacat cggtgccgcc aattaacacc ggcacacctg
    73021 gcgtgacttt ggcgatcaat acctgccgcc ctttctttgg cggtggacgg agatcaaatt
    73081 caatggtcgg ctggtaataa cccagcgctt tcagaccttc gcggatggca tcatcgacgc
    73141 gtgcgcgaaa gcgacggtct ggcgtcactt catcactttc aatcgtagaa agctgcgcac
    73201 gaacgttctt ttccagctgt cccgataacc cctcgacctg tagacggacg ttcgcggcga
    73261 cggcagatcc gcttaagcag agtaagctta cacagcataa ctgtcggata tagcgcacat
    73321 tttctcctga atatcctttt ttcctgcccc tggaacgccg ttaaacggct taacaaaaat
    73381 ccagtaatat ggattaaaaa gcagactaaa ccccaaatat ttcttatgtt ttactttaga
    73441 cctattcacg gtggttattg tgtgcaaata cgcctcttgt tacaacctta accccaatga
    73501 ccgattttcg ggagagcgac accatgagtt tatttgataa aaagcatctg gtttcccccg
    73561 ccgatgccct gcctggacgt aacaccccga tgcccgtagc cacgctgcat gcggtcaacg
    73621 gtcactcaat gaccaatgta cctgacggaa tggagattgc catttttgcg atgggttgtt
    73681 tctggggagt ggagcgtctg ttctggcagt tacccggcgt ttacagcacc gccgcaggct
    73741 ataccggagg ctatacgcca aatccgactt atcgggaagt gtgctccggt gatacgggtc
    73801 atgccgaagc ggtacgcatt gtttacgatc cttccgtcat cagctatgag cagttgctac
    73861 aggtattttg ggagaatcac gatcccgccc agggaatgcg tcagggcaat gaccacggca
    73921 cgcagtatcg ttcagcgatt tatccgctga ccccagaaca ggatgccgca gctcgcgcca
    73981 gtctggaacg ttttcaggcg gcgatgcttg ccgccgatga cgatcgtcac atcaccacgg
    74041 aaatcgctaa cgccacaccg ttttattatg ccgaagatga ccaccagcaa tatctgcata
    74101 aaaacccgta tggttactgt ggaattggcg gaattggcgt ctgtctgcca ccggaagcat
    74161 agcgttacgg gtacaaatgt agattgttga taaagtgctc tttgtctatg ccggatgcgg
    74221 cgtgaatgcc ttaccaggcc tacaaaatcg tccaaattca atatattgca gggactgcgt
    74281 aggcctgatc ggcatagcgc atcaggcagt attgcattta tcagcagtct gaattttaac
    74341 cctctggcga ctttacagta ccttacgcta tactagccac tgaaaatgcc ggttcacttt
    74401 cttcgaatcg gctttcaatg tgtatttcac acaaattaat caacttcccc ttccgaggat
    74461 ctggcctgaa aggtcggata agatatgtta aacagtattt tagtcatact ctgcttgatc
    74521 gctgtaagtg cgttcttctc gatgtctgag atctcacttg ccgcctcacg caaaatcaaa
    74581 cttaaactgc tggctgatga aggcaatata aatgcccaac gcgttctgaa tatgcaggaa
    74641 aatcccggca tgttctttac ggtggtccaa atcggtctga acgcagttgc gattctcggc
    74701 ggtatcgtcg gtgatgcggc attttctcca gcttttcaca gcctgttctc ccgctatatg
    74761 tcggcagaac tctctgagca actgagcttt attctctctt tctcgttagt gactggcatg
    74821 tttatcctgt ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc agaagcggtg
    74881 gctttgcgta tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc gctggtgtgg
    74941 ttcttcaacg gcctggcgaa catgatcttc cgtattttca aactgccaat ggtacgtaaa
    75001 gatgacatca cttctgatga catctacgcg gtagtggaag ccggtgcgct ggcgggcgtg
    75061 ttacgtaaac aggaacacga gctgattgaa aacgtctttg agctggaatc ccgtaccgtt
    75121 ccgtcttcaa tgacaccgcg tgaaaacgtg atttggtttg atctccacga agatgagcaa
    75181 agcctgaaga ataaggtggc ggaacatccg cactctaagt tcctcgtctg taatgaagat
    75241 attgaccaca tcatcggtta tgtcgattct aaagacctgc tgaaccgcgt gctggctaac
    75301 caaagcctgg cactgaacag cggcgtacaa attcgcaaca cgctgattgt gccggatacg
    75361 ttaacccttt cagaggcgtt ggaaagtttt aaaaccgcag gtgaagactt cgcggtgatc
    75421 atgaacgagt acgcgctggt ggtggggatc atcaccctca acgacgtgat gaccacgctg
    75481 atgggcgatc tggtcggtca ggggctggaa gagcagattg tcgcccgtga tgagaactca
    75541 tggctgattg acggcggcac cccaattgac gacgtcatgc gcgtgctgga tattgacgag
    75601 ttcccgcagt cgggcaacta cgaaaccatc ggaggcttta tgatgtttat gctgcgtaag
    75661 atcccgaaac gcaccgattc ggtgaaattc gccggctaca aatttgaagt ggtggatatc
    75721 gataactacc gcatcgacca gttgctggtg acccggatcg acagcaaggc caccgccctt
    75781 tcgccaaaac tgcctgacgc taaagataaa gaagaaagcg tcgcgtaacc cccagaaaca
    75841 tcaacggctc ctgaatcagg agccgtctta ttactgcata gcactttggt taagccatct
    75901 ctgtttgcag acgcataacc tgacggttaa cttcggacat cactgacaaa tgcagcttat
    75961 ccttcacttt tgggataaga atcttaccct tatcaaattc aaaagcgcca acgtccttga
    76021 tgtataaccg tccacggaac aggatcttta cgtacttcgc cacctgaagt gggttgtacc
    76081 gttggaaaat tttcattgtt atctcctgct gagtattacg cctgtgcggt gccacaatcg
    76141 gcccaactat tatgaggcgc aaattttaat gcctagtgac tatagactat ccgggcaatg
    76201 tttccaccgc gtataacttt ttttacctaa aggttacaat tattcagaat tatcttttta
    76261 ccgaagcgcg gtcttcagta taagcattca tttttcatat gatttgtgcg cttgaccgca
    76321 aactggcatc acacttgcgg gaaattcgat aaatagcaca tatgattaaa actcagaccc
    76381 aagtggtcgg atcacctgca tatcataaga aggaaacacc atgaccctac gcaagattct
    76441 ggcactcacc tgcctgctgt tgccgatgat ggcttccgca catcagttcg aaaccggtca
    76501 gcgagtgccg ccgattggca tcaccgatcg tggcgagttg gtgcttgata aagatcagtt
    76561 tagctacaaa acctggaaca gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat
    76621 tgctggtcgc acctctgcaa aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc
    76681 gaagttaccg cacgatcgtt accagaccac caccattgtt aacaccgacg acgcaattcc
    76741 gggttccggc atgtttgtgc gcagcagtct ggagagtaat aaaaagcttt atccctggtc
    76801 gcagtttatt gttgatagca atggcgtcgc acgcggtgcc tggcagctgg atgaaaaaag
    76861 ttccgctgta gtggtgctgg ataaagacgg tcgcgtgcaa tgggccaaag acggggcgct
    76921 tactcaggaa gaggtgcagc aagtgatgga cctgctgcat aaattaatta ataaatagac
    76981 actctgaacc caggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    77041 cagtcgtgaa cgtgcgctcc ggcagctgcg gccacagcat gtccagcggc ggtgtcccaa
    77101 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    77161 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    77221 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    77281 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    77341 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    77401 gcaatgttaa cggtgaattc gccattgcgt ttaataaact ctttagtacc atccagcggg
    77461 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    77521 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    77581 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    77641 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    77701 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    77761 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    77821 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    77881 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    77941 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    78001 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    78061 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    78121 gcgatttgat tcaggggagt ccgctggccg attacatgtc ggcgaaagga ttaaaagcag
    78181 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    78241 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    78301 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    78361 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    78421 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggaa
    78481 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    78541 aaggtgccga tgtcgttgtc gttctggcgc attccgggct gtctgccgat ccgtataaag
    78601 taatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    78661 ttggccatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    78721 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    78781 ttggtgtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    78841 aagcggaagc acgaccgatt tacgacatcg ctaataaaaa atccctcgcg gcggaagaca
    78901 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    78961 caatcggtaa atctgccgac aatatgtata gctatctggc actggtgcag gacgatccga
    79021 ccgtgcaggt ggtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    79081 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    79141 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    79201 ccgatcttta tctctacccc aatacactga ttgtggtgaa agccagcggt aaagaggtga
    79261 aagagtggct ggagtgctct gccggacagt ttaaccagat tgatcctaac agcacgaaac
    79321 cacagtcact cattaactgg gatggtttcc gcacttataa ctttgatgtg attgatggtg
    79381 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    79441 atgccaatgc ggaaaggatt aagaacctga cctttaacgg caagccgatt gatccgaacg
    79501 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    79561 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    79621 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    79681 tagcaccgat tgctggcgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    79741 aagccgcagc gtttattaaa gagaaagggc aatatccgat gaataaagtc gcgaccgatg
    79801 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    79861 ataaatcatc aaccgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    79921 ccctacaagc catgcaccgt agaccagata agctcagcgc atccggcagt tatgccgcac
    79981 gttcatcccg caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag
    80041 caaccttttc gctcacctgc tcgcccagcg gcgtgaggct gtattcaaca tgcggtggca
    80101 ccaccggata cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg
    80161 caagcatctt ttcgctcacc ccgccgattt tgcgccgcag gtcgctaaag cgatgagtac
    80221 cttcgcgtag cgccaccaga atcaacaccc cccaacggct ggtgacgtgt ttcaacacct
    80281 cgcgcgacgg gcactgttcc gcaaagagat taccctcttt cagttgttgc gacaggctaa
    80341 cctgactcat ttcatactta cctttttgta cgtacttact aaaagtaagt ttaggtgtta
    80401 gcgtatttaa actcaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca
    80461 ctggccaact tggtcactat gttattgaat ccttgatgaa aacggttcct gccagccaaa
    80521 tagtggctat cgttcgtaat ccggcaaaag cccaggccct ggcagcacaa ggcattaccg
    80581 tgcgtcaggc tgactacggc gatgaagccg cactgacatc tgcacttcag ggagtggaaa
    80641 aactactgct gatctcttcc agcgaagtgg gccagcgtgc cccgcagcat cgcaatgtga
    80701 ttaacgcggc gaaggcggcg ggcgtgaaat ttatcgctta taccagtctg cttcacgcgg
    80761 ataaatctcc gctcggcctc gccgatgagc acatcgagac ggagaaaatg ctggctgatt
    80821 ctggcatcgt ttacaccctg ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg
    80881 caccggcagc actggagcac ggcgtattta tcggtgccgc gggcgatggc aaaattgcct
    80941 cagcaacgcg ggcagattat gcggcagctg cggcacgcgt gattagcgaa gccggtcacg
    81001 aaggcaaggt ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag
    81061 agctgaccaa acagagcggc aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg
    81121 ccgcggcact gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg
    81181 acgttggcgc atcgaaaggc ggtctgtttg atgacagcaa aacgcttagc aaattgattg
    81241 gccgcccaac gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt
    81301 taattaaagt ggcatcctcc cgcatcctct ctgataatga cgggatgccg ggagcaatca
    81361 tgtctgcttc ctgaacgttt cttctgacag accaatggat gccagtaatg attagcggcg
    81421 tgctgtacgc cctgttagca gggttgatgt gggggcttat ttttgtcggg ccgttgatcg
    81481 tgccggaata cccggcgatg ttgcagtcga tggggcgtta tctggcgtta gggttaattg
    81541 cgctacccat tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt cgcgactggc
    81601 ttaccgcctt gatgctcact atgatgggca acctcattta ttacttctgc cttgccagtg
    81661 ccattcaacg tactggcgcg cctgtttcca cgatgattat cggcaccctg ccggtggtca
    81721 ttcctgtctt tgccaatctg ctttatagcc agcgcgacgg caaactcgcg tggggaaaac
    81781 tcgccccggc actgatttgt attggcatcg gcctcgcgag tgtgaatatt gctgagttaa
    81841 accacggact ccccgatttt gactgggcac gttatacctc tggcatcgtg ctagcgttag
    81901 tttccgtggt ctgctgggcg tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc
    81961 ccgacaaaca tccgatgatg tgggcaacag cgcaggcgct ggtcacactg ccggtttcac
    82021 ttatcggcta tctcgtcgct tgttactggc tgaatacgca aacgccggac ttctccctac
    82081 cctttggccc ccgtccgctg gtgtttatta gtctgatgat tgcgatagcc gtgctttgct
    82141 catgggttgg cgcactctgc tggaacgtcg ccagccagcg attaccgaca gtgattctcg
    82201 ggccgctgat tgttttcgaa acgctggcag gtttgctgta caccttttta ctccgccagc
    82261 aaatgccgcc gctaatgacg ctgagcggta tcgcgctgtt agtgattggc gtggtcatcg
    82321 cggtcagagc aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt tgacacgctg
    82381 gcagtgagtt aaataagcct ctgctacgta agggttatag cttttgcctt aaagatgcat
    82441 ttaaaataca tcttatctta ttaagaatga ggtatcagct atggcttatc gcgaccaacc
    82501 tttaggtgaa ctggcgctct ctattcctcg cgcttcagct ctgtttcgta aatatgatat
    82561 ggattactgc tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta aagaactgga
    82621 tgttgaggtc attgaagctg aactggcaaa gctcgctgaa caaccgattg agaaagactg
    82681 gcgtagcgcc ccgctggcag aaatcattga ccatatcatc gtgcgctacc acgatcgtca
    82741 ccgcgagcag ttgccggagc tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga
    82801 caaaccgagc gtgccaaaag ggctgacaaa atacctgact atgctgcatg aagagctttc
    82861 cagccacatg atgaaagaag agcagatcct cttcccgatg atcaaacaag gcatgggcag
    82921 ccaggcaatg gggccaatca gcgtaatgga aagcgagcac gatgaagcgg gtgaactgct
    82981 ggaagtaatc aaacacacta ccaataacgt cacaccgccg ccggaagcct gcaccacctg
    83041 gaaagcgatg tataacggca ttaatgaact gattgatgac ctgatggatc acatcagcct
    83101 ggaaaacaat gtactgttcc cacgcgcgct ggcgggtgag tgatctaaaa gctggatggc
    83161 attgcgccat ccagcatgat aatgcggtta tttccgcagt tcagcagccc gcttcttacc
    83221 aataaacagc cagcccaacc ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg
    83281 gcgagtgtca tcttccagtg tcagcaacac gaccacgaac acaaagaaca ccatacatac
    83341 ccagcacatc agcttgccga gcggcatctt gtagatcgac ttctcatgca gatgaggacg
    83401 ctgtttgcgg tacaccaggt acgagcaaag gataattgtc cagacgaaca taaacagaat
    83461 cgcggaaacg gttgtaatca tcgtgaacgc gccaatcacg ctaggattca catacaacat
    83521 caccacgccg ccgagcagac agatacacga gaaggtcaac cctttcgcgg gtactgcgcg
    83581 cttagaaagt ttagcgaacg ctttcggcgc aacaccttcc tgcgccagac caaacagcat
    83641 acggctggta gagaagacac cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa
    83701 gttgatcacg ctggcagccg caggcaaacc taccaacaca aacagctcaa cgaacgggct
    83761 tttctccggg actaccgaac tccacggcgt cacggacata atcacaatca gcgcgaagac
    83821 gtagaacata atgatacgga tcggaatgga gttaatcgcg cgtggcagtg atttctccgg
    83881 atctttggtt tccgcagctg tagtacctac cagctcaatc cccacgaaag cgaaaaccgc
    83941 tatctggaat ccggcaaaga agccacttaa acctttcggg aaccagccgc cgtcattcca
    84001 caaatgcgcg aatgacgctt ccacaccggt cggtgactga aagtgcatcg ccaccatgac
    84061 caggccgacg acaatcagcg acacgatggc gacgattttg atcatcgcaa accagaactc
    84121 catctcaccg aacattttca cggtggcaag gttgagcgtc agcagcagca ctatcaccgc
    84181 cagcgaggcg acccagtcgg agagatcggg gaaccagaac tgagcataag ccgtgatcgc
    84241 caccacgtct gccataccgg ttacaaccca gcagaaccag taggtccagc cggtgaaata
    84301 tcctgcccac ggcccgagta aatcggaagc gaagtcacta aaagatttgt attccagatt
    84361 cgaaagcagc aattccccca ttgcccgcat cacgaaaaag agcataaaac caatgatcat
    84421 ataaacgaaa atgatcgacg gcccggcaag gctaatcgtt ttgccggacc ccataaacaa
    84481 ccccgtacca atggcaccgc caatggcaat aagctgaata tgtcggtttg tgagattgcg
    84541 ccgtagcgac tgttcagccg gagcctgatc atcggcaacg acttttacct gatctaccat
    84601 gtttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta gtcagtctat
    84661 gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg ggatgaatac
    84721 taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca cggtgataac
    84781 gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa tagcgccgtt
    84841 tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag gggaacagaa
    84901 tgcgctgctt agagaatttc cagcagttcg acttcaaaca ccagggtact gaacggaggg
    84961 atggatgcgc ctgcgccgcg ctcgccatat gccagttcct gcgggatagt cagttcccat
    85021 ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac gccattaacc
    85081 ggaaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc gtcgatcagt
    85141 ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc ttcaccctgg
    85201 ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt tttggcgttt
    85261 tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg acgaacggca
    85321 tcggcgcgct cgtggatttc acgcagcgcg cgatgcacca catcaaccgg aacagccgga
    85381 tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag cagcccttcc
    85441 aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct
    85501 tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta taaaagtagc
    85561 gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg atgacaaatc
    85621 ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg ggtttaccac
    85681 ggacatcata gcgttagctt attcataggc tatgattgag gaacaagacg cggagcagga
    85741 ggaaaccatg cccgggcgct ttgaactaaa accaaccctg gagaaagtct ggcacgcgcc
    85801 ggacaatttt cgcttcatgg acccgctgcc gccgatgcat cgtcggggga tcattattgc
    85861 cgccattgta ttggtggtcg gatttctgct tccatctgat gatacgccca acgcgccggt
    85921 ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca caaccgccga cagaagaaca
    85981 gctgcgggca caattagtca caccacaaaa tgatcctgat caggtggctc cggtcgctcc
    86041 agaacctata caggaaggcc agccagaaga gcagccgcaa actacccaaa cgcaaccatt
    86101 ccagccagat agcggaattg ataatcaatg gcgctcttac cgtgtcgaac ccggtaaaac
    86161 gatggcgcag ctgttccgcg accacggact gcccgctacc gatgtctatg caatggcgca
    86221 ggtagaaggc gcgggtaagc cgctgagtaa tttgcaaaac gggcaaatgg tgaaaatccg
    86281 ccagaacgcc agcggcgtgg tgacgggctt aaccattgat acggggaata atcaacaggt
    86341 cctgtttacc cgccagccgg atggcagttt tattcgtgcg cggtagatga agggtcaacc
    86401 aaaagccagc taaggattgt ctctttgatg accttacgtc tggcttccag tgcctggggg
    86461 gaaaccatac tgatatgata aaccaggcca agcgtatgct ggttgatcaa ataatatccg
    86521 ccaagcgcag caatattaat attcacctgc aatgggtcga tatctggctt aaaaatattg
    86581 tgtttcttcc cttcatccaa taaggattcc attaactgca agtgcgcatg gttaatttct
    86641 aagagacgct gtgattttgc ataatgcaca cctttacttt gattttcact atgcacaatt
    86701 tttaaaaacc aggggttagc aatataataa tcccatgtaa aatcaataat tttctcaatg
    86761 gctttttgtg gcggaagatc tgagaaatca agggcctttt ctttttgtcg aatatcgttc
    86821 caggtatatt ccagtacttc aataaataaa ttttctttat taccaaagtg atgataaacc
    86881 gtctgtttac tacaccccgc agccttaaca atattatcga ccctggcccc ttcataacca
    86941 tattcggcaa attcattaac cgcgcataac agcagctttt ccttcaggct ctgtacgttg
    87001 ctggtatcaa gatacataac gcttccccac gacctcttca agataactat cttaacccga
    87061 ttaattagaa gctccagtaa aaaggataaa aacttgtact ttcatcaagt tatcaaatgt
    87121 cctgtgatca ctatcgcata tcaaccaacc agatggttga tctttcttgc ggcacgtcgc
    87181 aagctaattg tcacttagaa aacaatattc aaaaaaagag agctattgta tgaaatccac
    87241 tcgtcctgtt gccgtgatta ccggagccgc tcgtggtatt ggaaagggct gtgcgctgga
    87301 gcttgctcgt ggcggattta acctgctgat taacgatctt cccgatgccg acagcgtaga
    87361 aaaactgcat atcacacaac aagaatgtat agctgaaggc gtggaagtga tttgcttccc
    87421 tgccgatgtc ggcgatctct ccttgcatga agagatgctc gacgcggcgc aaaaccagtg
    87481 gggacgtctg gactgcctgc tcaacaacgc aggcatttca gtgaaaaaac gcggtgacct
    87541 tctcgacctc gaaccagaca gcttcgacca aaatattgcc attaacaccc gcgcgccgtt
    87601 cttcctcgct caggcattca gtaaacgtct gctcgcacag ccgaaaccgg aagcagaact
    87661 gccgcaccgc tcgattattt tcgtcagttc catcaacgcc atcatgctgg cgatgaatcg
    87721 cggcgaatat accattgcca aaaccgccgt ttctgccgct gctcgtctgt ttgctgctcg
    87781 cctttgtaat gaacaaattg gcgtttatga agtgcgcccc ggtctcatca aaaccgatat
    87841 gactattccg gcaacggctt attacgacga actgattgcc aaaggtctgg tgccgtgggg
    87901 acgttggggt tatccggctg atattgcttc cactgtccgc gcgatggcgg aaggtaaact
    87961 gatttacacc tgtggtcagg cggtcgccat cgacggcggc ttaagtatgc cgcgcttctg
    88021 aggctgaatc atgagagatc tgttacaacg cccggattta ttcagtatca ataccgcaac
    88081 actgggctat aaaacgccgc tacccgccat tattgatgcc tgcgccgcgc gtggtattgg
    88141 cgcgattgct ccctggcgca gggagttgca aggtgaagat ctccagcaaa tcgcccacca
    88201 gcttgccgcc agtaacatga gcgtttccgg tttgtgccgt agcacttact acaccgcgcc
    88261 gacactggcg gaacgcaaac tggcgattga tgataaccgc cgggcgctgg acgacgcagc
    88321 ggtgcttaac gccgcctgtt atatgcaggt ggttggcggt ctgccaacgg gcacaaaaga
    88381 tctgtacgaa gcgcgggaac aagtaaaaca agggattcgc cagttgctgc cacattcgaa
    88441 agatgtcggc gtgccaattg cgctggagcc actgcacccg atgacagctg ccgatcgctc
    88501 ttgcctgtgc acgttacgcc aggcgctgga ctggtgtgat gaactcgatc ccgatggtga
    88561 gtttggcctc ggcgtggcgg tggatgttta tcacgtctgg tgggatccgg atctcgccag
    88621 tcagatcctg cgtgccggaa aacgcatcct cgcgtttcat gtttccgact ggttagtgcc
    88681 gaccactgat ctggtcaatg accgtggtat gccgggagat ggcgtcatca atattccgtc
    88741 aattcgtcgg ctggttgaaa acgccggatt taatggtgct attgaactgg aaatattctc
    88801 gccttactgg tggcagaaag atattaacag cacgctggat attagcgtcg atcgcatcgc
    88861 gcattattgt taaggaatga tcatgagtga ccaacccgtt ctgttcagca gagtggcggc
    88921 ttcctgccgc ctgacattga accgtgaaga taaatgtcat gctattaacg aagagatgat
    88981 cgaatctctc gaccattatt taaatgaaat tgaaaacgac accacattac gattagtcga
    89041 attaacggca accggcgata aattcttttg tgcgggcggc gatattaaat cgtggtcggc
    89101 ctattcgcca ttagatatgg gcagaaaatg gattaaacgc ggtaatgacg tttttaatcg
    89161 cctgcgaaac ttaccccaat taaccgttgc gaatctcaac ggtcatacca tcggcggtgg
    89221 tattgaactg gccttgtgct gcgatattcg tattgcccgc cccggcgcga aattctctaa
    89281 cccggaagtg atgctcggca tggtgcctgg ctggatgggc attgaacgtg tgcttaatca
    89341 ggttggtccg gtggtcggtc gccagatgct gatgctgggt aaacgcctga cggcgcagga
    89401 agcccaggcg gcgaatttaa ttgatgaagt ggtagaaaaa gaacaggtgg aaagctggat
    89461 ggcgaaccag ttagcgcagc tggaaaaatg cggtccggtg gcgcttgcac atattaagca
    89521 actgattctg gcactggaaa ataaacatgc cgattacccg caccagctac tggccggatt
    89581 aatgtccgcc actcaggatt gccagcaggc gacgcgcgcc tttgccgaaa aaagtagcgt
    89641 aagcttccat aatcaataag gaaattgtat gcgtaagata acaaccgccg aggcactggc
    89701 ggcgcaaatt caggacggtg cgactattgc tattagcggt aacggcggcg gtatggtgga
    89761 agcggaccat attctggctg ctattgaagc gcgtttcctg caaaccggac accctcgcga
    89821 cctgacgcta atccactcgc tgggtattgg cgatcgcgac tgcaaaggca ctaatcgttt
    89881 tgcgcacgca gagatgctca aacgcattat tgccgggcac tttacctggt cgcccaaaat
    89941 gcaggcgctg gtaaaaaata acacgattga agcctactgc tttcctggtg gcgtgattca
    90001 ggcactgcta cgggagatcg gcgccggacg tccggggctt tttacccacg ttgggctggg
    90061 atcgtttgtt gatccacgca atggcggcgg taagtcgaat gaatgcacta ccgacgacct
    90121 ggtagaactg attgaaatcg atggtgaaac caaacttcgt tatcgccctt tcaaagtgga
    90181 ttacgcgatt ttgcgtggca cttatgccga tcctcgaggc aacgtcagcc ttgaagaaga
    90241 agcgattgat atggatagct attccatggc gctggcagca cacaacagcg gcggcaaagt
    90301 gttcgtacag gtacgcgatg tgctggaagc tggcgcgatt gaaccacgcc gggtcaaatt
    90361 accgggcatt ctggttgatg gcatcgttga acaccgcgaa caaccgcaaa cctatcttgg
    90421 cggttacgac ctgaccatta gcggtcaaca tcgccgtctg agttctaacg acgctattga
    90481 actggttagt catccggtgc gccgcctgat tgcccgtcgg gcagcacgcg aactggtggc
    90541 tggcgcttca accaactttg gctttggtat tcctggcggt attccaggcg tagcgctgcg
    90601 cgaaggcgtt ccttaccaaa gtttatggct gagcgtcgag caaggcgtac ataacggcat
    90661 gatgcttgat gatgcgtttt tcggctgcgc ccgcaacgcc gatgccatta ttccatcact
    90721 cgatcaattt gagttctaca gtggcggagg gatcgatatc accttcctcg gcatgggaga
    90781 gatggatcag tacggtaacg tcaacgtctc ccatctcaat ggcaatctca ttggtcccgg
    90841 cggttttctc gaaattgcgc aaaacgcccg taaagtggtg ttctgcggca cgttcgacgc
    90901 caaaggtagc aagattgatg taacgccaga tggcttgcat atcacccagt caggtcaaat
    90961 ccctaaactg gttacccagg tggaaaaaat cacttttagc gccgcctacg cacagcaaag
    91021 tggtcaggaa gtgttgtata tcactgaacg cgcagtattc cagttaacgg cagaaggcgt
    91081 tgaattaatt gaaatcgcgc cgggtgtgga gattgagcgc gacattctgc cgtatatggc
    91141 tttccgtcca attatcaagc acccacgcct gatggaaagt agcctgttta cgccgatgga
    91201 ggatgcatga gtcagcttga tgacaccatc cttgatgcgc tgacgcacgt tactttccca
    91261 aagggttttg cacaggcaga gcccgcatgg gttgtcacgg tagacggtgt tgattaccca
    91321 ctctggcaaa cagatgccct ggtagtcggc agtggtgcag caggattacg tgcggcagtt
    91381 gaacttaaac gccgtcagca aaatgtgctg atcgctaccg ccgggttata tatggggacg
    91441 tcggcatgtt ctggttccga taaacagaca ctatttaccg ccgctaccgc gggcaacggc
    91501 gacaacttca ccaaactggc agaggcactg gcgagcggtg gggcgatgga tcacgacacc
    91561 gcttatgtcg aagcggtagg ttctctgcac actctaggcg ggttgcaata tcttggtctg
    91621 gaattaccgg aagatcgcta tggcgcgatt cttcgttatc aaaccgacca tgacgaagcc
    91681 gggcgtgcaa cctcgtgtgg gccgcgtacc tcaaggttaa tggtgaaagt gctgttggaa
    91741 gaagtacagc gcctcgccat tccagtgttg accagtgcaa cagtgattaa actgctgcat
    91801 cagcgtgacg aaaacggcga agaccgcgtg gcaggggcaa tcctcgcgac cggtcatcgc
    91861 gcccataacc catgggggct ggcaattgtg actgcgccca atgtggtact ggcaacgggc
    91921 ggaccgggcg agctttatcg cgacagtgtt tatccacata aatgcttcgg ctcgctgggg
    91981 ctggcgctgg aggaaggtct gacgctaacc aatctgaccg aaagccagtt tggtataggc
    92041 acgccgcgta gcacgtttcc gtggaattta tccggcacct atgtgcaggt gatcccgtat
    92101 atctattccg tggatgctga gggtaacgag tataacttcc tcgcggatta ctatcgcacc
    92161 acccaggagc tggcttcaaa cattttccgt aaaggctatc agtggccgtt ccacgccact
    92221 cgggtgatgg attttggctc cagcttgtta gatatggcag tagcgcaaga gcagcaatca
    92281 gggcgtcagg tatttatgga tttcaatcgc aatcctgaag cggtgccggg tgacctgccg
    92341 ttctcattag atcgactgga cgacgacgtt cgcgcgtatc tggaaaataa cgacgctctg
    92401 gcaccatcgc ccatcgaacg gctgcaacga atgaatccgc tgtctatctc gctgtataag
    92461 atgcacggtt acgatctcac cacacagcct ttgcagtttg ccatgaataa tcagcatatg
    92521 aatggcggca ttgaagtgga tatctgggga caaacctccc tgcccggctg tttcgccgtg
    92581 ggggaagttg ctggcacaca cggcgtcact cgccctggtg gtgcagcatt gaatgccggg
    92641 caggtttttg ctgttcgtct ggcacgtttt attggttgca cgcaaaagcg gaatattgat
    92701 ggagatatag cacagctggt agctcctaca ctggcttcta taagagagat cattactcag
    92761 gcgcacgata acgggaccgg gatgccgttg tcggttgtga gagaaaaaat tcaggcacga
    92821 atgtctgacc atgcgggatt tatttgccat gccgataaag tccgacgcgc cactcgcgat
    92881 gccctgctat tgagcgaatt tgtccaacgg catggattgg ctatcaaaca tgtgggtgaa
    92941 gttgccgagc tgtttatgtg gcggcatatg gcactgactt ctgccgccgt attaacgcaa
    93001 ttaacgcatt atattgacgc aggtggtggt agtcggggag cacgtattat tctcgacagg
    93061 gatggaaatt ctattccgca aacacgaaat ggtttttgtg atgcctggcg ctttcgttca
    93121 gagcgtactg aggataaaaa agataaacta cttattcatt attgcaacgg tatctttcac
    93181 gtcagagaaa cacctgttcg tgagtttcct attatccgag gtatttggtt tgaaaaaaac
    93241 tggcctggtt ttcttaatgg gacaatttac cagccacagg atgaatgata ttgcaaaggt
    93301 taatcactat gcgaaatata attagatcta ttaatgctct acttgcagca ttgaacatta
    93361 ccattttggc gatcatcgtg gcatgtgtga cgtggcaggt ggctgcacga tttatattta
    93421 ctagtcccag tatttttaca gatgaactca gccgtttgct ctttatttgt cttggacttt
    93481 ttggtggggc atacaccgca gggcaaaacc ggcatctggc gattgacctg ctacctatga
    93541 tgctgaaagg aaaagcccga cgtcatttat ttttgtgtat tcagattatc gttattatct
    93601 tcgcaaccat cattatggta tatggtggtg ggctattaac gatggataca ttcgattcag
    93661 gtcagacctc gcccgcttta ggctggcaaa tgggatatat ctatatgagc atcccgatta
    93721 gtggtgtact tattattata tacaccatag atatggtttt aaccgaactt aaacaaccac
    93781 tctaaagata acaaacaaca accaaccata gtaggaatgg agaaattatg gattactggt
    93841 taccgattat tgtgttattt ggtgccttct tttttatgtt ggcattaggt gtacctattg
    93901 tttatgccat tggtttatcg acactggcat caatttccac acaactcgat ttcaactcag
    93961 cattgagtgt ggtttcccaa aagttagcct ccgggttaga tagttttacg ttgttggcaa
    94021 tcccattttt tatcttgtct ggcaacatta tgaaccatgg aggaatagcg cgacgattaa
    94081 tcaactttgc ccgtattctc ggcggtcgtc tgccaggctc acttgcccac tgtaatattc
    94141 tcgccaatat gctgttcggg gcaatttctg gttcagccgt ggcttcggcg gctgcgatgg
    94201 gcggcgtcat gcatccgcaa caggttaaag agggttacga cccggcattc agtactgcgg
    94261 ttaacgttgc ctctgccccg acgggtttgc ttattccacc aagtaatacg ttaatcgtct
    94321 attcactggt cagtggtggt acttctattg ccgcactgtt cctggcgggt tatgttccgg
    94381 gaattttgct gggtctggca ctgatggtaa tagccggtat tatcgcggta cgtcgcggtt
    94441 atccaaagcc agaacgcccg actttacgcc aggctggcgt tgcgatatgg atggctatcc
    94501 ccagtatttt cctgattatt ttaattatgg ggggtgtatt aagtggtatt tttaccccga
    94561 cagaggcctc cgcaattgca gtaatttata cccttttcct tgcactggtt ctttatcgtg
    94621 aaatctcagt taaagattta cctaaaatat ttctggaatc cgttattacc actgcaattg
    94681 tcttgttatt aattggttct tcgatgggta tgtcatgggc aatgtcaaac gctgacgttc
    94741 ccttcctgat tctcgatttg cttaacacta tttctgacaa cccaataata atcctgctta
    94801 ttatcaatat tatcctgttg attatcggca cttttatgga tatgacaccg gcggtactga
    94861 tttttacgcc gatattttta ccggttgtca ccgaactggg catggacccg attcactttg
    94921 gtattgtcat ggtactgaac atgtgtatcg gtatttgtac tccgcctgta ggcagtgtgt
    94981 tatttgtcgg ttgtagtgta tctaaattac cgattaataa aattatcaaa cctatgctgc
    95041 cattctatgc cgtgatggta ttggtgctgg caatggtgac ctatattccg caaatcagta
    95101 tggcattgcc aagagcactg ggctactgat gcgcattcac tggcggcacc tgccgccata
    95161 tttattagaa aaggaacatc tatgaaaata aaagtatccg ctggcattat cggtgctgtt
    95221 cttatgttat ccgcaagcca gtcctgggca gtgacattaa aactgagtca taatcaggat
    95281 aagtctcatc ctgttcataa agcgatggag ttctttgcga aaaagagcaa agagtactct
    95341 aacggtgata ttactattcg tatttatcca aatggaacat tgggtactca acgagaaaca
    95401 atggagctga ttcgttctgg cgctattccc ctggtaaaaa ccaatgcggc agaaatggaa
    95461 gcatttgaaa attcctataa attatttagc ctgccttatt tgttccgcga tcgtgatcat
    95521 tattatcagg tcatgcaggg cgatatcggg agaaaaatcc tcgactcaac gaaaagcaaa
    95581 ggttatttcg ggctgacttt ttatgatgga ggcgcccgca gtttctatgg caataaacca
    95641 gtactgaaac cagacgatct gaaaggcatg aaagtccgtg tccagccaag ccctggcgca
    95701 gttgaaatga tcaaagtcat gggcggtaac ccgacgccac tggattacgg cgagttgtat
    95761 acagccttac agcagggtgt ggtcgatatg gcagaaaaca gcgtgatggc gctgaccacc
    95821 atgcgtcacg gtgaagtggc aaaatccttc agccttgacg aacacactat ggttcccgat
    95881 gtggttctga tgagcaatgc tgcgtttgat aaacttagcc cggaaaatca ggcagttata
    95941 ttaaaagcag ctaaagaatc aatgagctat atgaaagact tgtggagcga ggaagagaaa
    96001 caagaatttg cgaaactgga taaaatgggc gtgaaagtct accaggtaga taaagctccg
    96061 tttatcgaga aagtacagcc aatgtacgca aacttcgcta aggacaaccc agcccttgcc
    96121 ccaatgctgg ctgatattca ggcagctaag taattatctt cgtagggctg cttattgtgg
    96181 cccttatcga tatctgcaag tgctcgctac aaaagcaaaa cgccgaccaa tggtcggcgt
    96241 ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa
    96301 tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt
    96361 cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat
    96421 gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt
    96481 aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca gaacttcagc
    96541 cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc
    96601 tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt taacgtttac
    96661 ctgatcaccc aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt
    96721 atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc
    96781 agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca
    96841 cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc
    96901 gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg
    96961 cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc
    97021 ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg
    97081 aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt cgtgtccgct
    97141 aacaataacg ggcatttgac accatgcctg ccggtgaaag ccggcttcct cctgcacaga
    97201 acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg
    97261 agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga
    97321 atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg
    97381 ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac
    97441 catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc
    97501 ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt tgatcgggta
    97561 agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat
    97621 ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat
    97681 aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct
    97741 gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg
    97801 ttatagtact tgtatgcccc gctaaactca agggcagagc cgaaataaat ctcccctttc
    97861 gcaattaacc tctaaaccgc tgatttggag ttcatcacac ttcatcatgg tgtttttttg
    97921 aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag
    97981 aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc
    98041 gagttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa
    98101 tcaataacca ccaggccaga aatatggacg atatgcaaag cttaggcgtg atttatatca
    98161 accataattt cgccactgaa agtgaagcac gtcaggcatt aaatgaagaa acagatgcgc
    98221 agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc
    98281 acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt
    98341 tgctttgccc ccttcgcagg gggctttttt tgaaaaacgg ctgcggaatt agaccagtta
    98401 tctcccgaag aaggaaattt ccgcagcgcg tgttttactt ctgcccgtaa taagcgttag
    98461 gaccatgctt acgcatgaag tgtttattca tcaggaagct gtcgatgtga ttgagttgtg
    98521 gattaatact gcgcgcaatc cacgccattt tcgccacttc ttccatcact accgcgttat
    98581 gcaccgcgtc gtgggcatct ttcccccagg cgaacggtcc gtgctgatac accacaattc
    98641 ccggcgtatg cagcggctcg gcgttgccca gcgtttcgat aatcactttg ccagtgttca
    98701 gttcatactc accctgcacc tcttcttcgc ttaatccacg cgtacacggg atgtcgccaa
    98761 agaagtagtc ggcatgcgta gtgcctaacg ccgggatcgc cagccccgcc tgcgcccatg
    98821 cggtagcatg agtggagtgg gtatggacaa tgccgccaag cgacggataa cgacggtaga
    98881 gttcaagatg cgtcgcggtg tcggaagacg gacgatattc cccttccacc accttaccgc
    98941 tcatatcgac caccaccata tcgtctgctt tcatggtttc gtaggcgacg ccgctgggct
    99001 tgatcaccac cagcccgcgt tcgcggtcga tagcgctgac gttgccccag gtaaaggtca
    99061 ccagcccgta gcgcggcaga tccatgttgg cttcaaatac ctgctgtttt agcttttgca
    99121 ttatgccgcc tccaccatgc ccgctttcgc catgcgcgct ttcacccaat cacgcgcttt
    99181 cgccacttct gccgccgggt cttccgccgt ttcgctccac atctcaatca ggtacggccc
    99241 gcaatagcca ctctgtttga gcgtttcgaa acaacgttcg aaatccacta caccttcgcc
    99301 aaacggcacg tttttgaaga cgccaggttt ggtgtctttc acatgtaccg cgacgatatg
    99361 cccgattccg gcctgcaact ccatctgcac atcgttgtcc cacgccgaca ggttgccgat
    99421 atccgggtag agctggaacc acgggttgtt gagatagtgc gcgtaaccca gcgccttgct
    99481 gatggagttc atcaacggat aatccatgat ctccatcgcc agcgtcacct gcgcgcggct
    99541 cgccatctca acgctctctt tcaggccgtc acggaaacga cgacgcgttt cgttattggc
    99601 ttcctgatag taaacgtcat agcccgccag ctggatcacg cgaatgccga cgtcctgggc
    99661 gaactggata gctttacgca taatctccag cccctgcgcc cgcaccgcgt catcttcact
    99721 gcccagcggg aaacgacgat gagcagaaag gcacatggac ggcacgcgca cgccggtttc
    99781 aacaatcgca ttcaccagcg ccagacgctg ctcgcggctc cagtcgaggc gcgacaggcg
    99841 ctcgtccgtt tcatctaccg acatctcgac aaaatcgaag cccaacgttt ttgccagttg
    99901 caggcgttcc agccagcact ccccggcggg gagcgctttt tcatagatgc caagcgggat
    99961 ttgtttggac aacatatccg ctccttagcc ccacagttcg gcgatggaac gtttgaactg
   100021 acgcgcggct tcaaccggag aagcggcatc acggatgctg cgaccggcga taaacacatg
   100081 aattggaatg cctttgaaca tcggcagatc ttccagcgcc aggccaccgg taacggtgac
   100141 tttgaagccc atatcggaaa gacgtttgat cgcagtgata tcggcttcac cccacgccac
   100201 gccagcggcc tgcgcgtcac ggctgcggtg ataaaccacc tgctgaatac ctgcgtcgcg
   100261 ccactgttgc gcctgttccc aggtccagta accggtcagt tcgatctgca cgtcgccgtt
   100321 aaactctttt gctacgtcca gcgcaccttt ggcggtgttg atatccgcac aacaaatcac
   100381 ggtcacccag tcagcgttgg cttcgaagca catacgagaa agaattttgc ccgcatcggc
   100441 aattttggcg tctgccagca cgattttgtg cgggtagagc gctttcagat cgcgaaccgc
   100501 acgcacgcct tcgcccacgc acagaatggt acccacttcg ataatgtcga cttcttcggc
   100561 aatcaggcga gtggtttcgt aggcgctatc catagtctgg ttgtccagcg cgacttgcaa
   100621 catcggtaat gacatcttca attccttctt aagctgccgc gttggtgcgg tcaatgagat
   100681 ccagtacttc ctgctcggta cggcaggcac gtaaacggtc gaaattttcc tcatcttcaa
   100741 acaggttgac gatctgcatg atgcccactt cctggtgcgt gttggcatcg accgccgcca
   100801 tggtgatgag gatatccacc gggtcgttat cttcgtggtt gaactccagc ggctttttca
   100861 gcgtcaccag cgcaaaaccg gttttcttaa caccctcttc cgggcgaccg tgcggcattg
   100921 ccaggccagg tgcgagcaca aagtacggac cgtgctgttc aaccgcatcc agaatcgcct
   100981 ggtagtaacg cggctcgaca acatccgccg ccaccagcag gtcaacgccg atttttactg
   101041 cgtcctgcca tgtctcagct tcagcctgca ggcggatgga tttattttcc gccagcgaat
   101101 cacgtaattt catggcgcgt ccttacttca catcctgcgg gaaatgctct ttgatcactt
   101161 ccagcagttt cgggccaaaa tcggcaggag agagcatgtt gcgcacgcca accacgtatt
   101221 tgttgccggt cacggagatc tcgcccgcga tgtgcgtgga agcgatgata atatccgcgt
   101281 tgctcagctc acttttgtac tcgccaaccg cgcagctgtt taccgtgtgg tcaatctttg
   101341 attgggttaa aaactggtcc actttcattt tcatgatcat ggaactacct tgtccgttgc
   101401 cacacacagc cagaatacgt acggtcataa tcaaaactcc ttattaagca gactgttctg
   101461 ccagttgttt ttctgcatct tcttcagcgc gcagtgcgcg gccagcgaag aacatataag
   101521 ccagtgcaat tacaatgatg acggccataa aggcgatacc gatggagaag aagccctgca
   101581 tcatcggcgg tgccagaatc gaccagtccg ccatgcccat ccaggcgctc ataccggtga
   101641 gtttcaccgc ccagacgcag ccaaagattt caatcatccc catcaccaga catatcttca
   101701 gcgccgcacg ccagccgccg aagtggttag cgaacacgcc gatggtggcg ttggagaaga
   101761 acatcgggat aaagccggga ataatcagga tcgaggaacc gcaggcgacc aggatgccaa
   101821 ccgcaatcag ctgaccgatg gtgccccaca taaagcccca aaccacggcg ttcggcgcga
   101881 agctatagat agccgcacag tcaatcgcca gaaccgcacc agggatcagg cgctgggaga
   101941 taccgttaaa tgcttcagag agttccgcca caaacatgcg cacgccctga gtgatgatga
   102001 agatcgccac cgcgaaggag aaaccagttt gcaggatgta caccgtccag ttcactttgc
   102061 ctgccatcgc ctgcacggtg tcgataccga aggagagcag aatggcacca aagaagatgg
   102121 tcatcacaat cgccgtggag acgatgttgt cgtggaaaat attcagccag cctggcagtt
   102181 tgaggtcttc aacgctttcc tcttttttgc cgaggaacgg cgcaacttta taggcaatcc
   102241 atgatgcaaa ctgttgctgg tgaccgatgg agaaaccgca gccgtccgtc acttcctgag
   102301 tcggcttgta cattatgttg gaggtaatgc cccagtagag cgaaaccaga atcgccgtgc
   102361 agataatggt cgtccacatg gagtagccga aaataaacaa cgtaacggca atcagccccg
   102421 cctgctggaa catgatatga ccagtcagca tgatggtacg aataccggta atgcgatgca
   102481 gcagcacgta acagatgttc aacgccagcg ctaacaacac cgcgtaacct acccagttat
   102541 aggcatcgcc catgcggtcg atggttgcca tcatcgacgc gtaagtatcg gaaattgcgc
   102601 cgttaatgcc gtagacttcg gacattttcg ccaccaccgg tttgaaggtg ctggtgagga
   102661 tgccggaccc tgcctgcaac aacatgaaac caattatggt tttaatcgtg cctttaataa
   102721 taacgctgac acttttgcgc agtaggatgt agcccagaca ggtcacaata cccagcaaca
   102781 acggggcatt ggtcatgacc tggttaaaaa acacggtaaa gatgttgtag aggatctcca
   102841 taacgatctc ctgaagaaga ggtaaccggg tattccacac acccggaatg ttgtgcactc
   102901 actctaattt tcaaaagtaa tcacaacaag attatttatg attaaatgtg acgcgacccg
   102961 caaataattc cacatggata gaatgggatt cacacaacga aacaactatt catttaaatt
   103021 catttagtta attgaattac acattccatt aaatctttcc acactcaaaa tcaacaacca
   103081 cttccctggc aaaaagtcaa aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt
   103141 aatcaaaacc aatcacatat tgattcgaat tgaactatca tgaaggtaaa tggcgatgag
   103201 taaagtgaaa agtatcaccc gagaatcctg gatcctgagc actttcccgg agtggggtag
   103261 ctggctgaat gaagaaattg aacaagagca ggtcgcccct ggcacatttg cgatgtggtg
   103321 gctaggctgc accgggatct ggttgaaatc agaaggtggc accaacgttt gcgttgattt
   103381 ctggtgcggc actggcaaac aaagtcacgg taacccgtta atgaaacagg gtcaccagat
   103441 gcagcgcatg gctggcgtga aaaaactgca gccaaaccta cgcaccaccc cgtttgttct
   103501 tgatccgttt gcgattcgcc agatcgacgc ggtactggcg actcacgatc acaacgatca
   103561 tatcgacgtt aacgtcgctg ctgccgtgat gcagaactgt gctgatgacg taccattcat
   103621 tggaccgaaa acctgcgttg atttgtggat tggctggggc gtaccgaaag agcgttgcat
   103681 cgtggtcaaa ccgggcgatg ttgtaaaagt gaaagacatt gaaattcatg cgcttgacgc
   103741 tttcgaccgt actgcactga tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc
   103801 ggacggcatg gacgatcgcg cggtgaacta tctgttcaaa acgcctggcg gctccctgta
   103861 tcacagcggc gactcccact actctaacta ttatgcgaag cacggtaacg aacatcagat
   103921 cgatgtggcg ttaggatcgt atggtgagaa cccgcgcggt attaccgaca aaatgaccag
   103981 cgccgatatg ctgcgtatgg gtgaagccct gaatgcgaaa gtggttatcc cgttccacca
   104041 cgacatctgg tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa
   104101 aaaagatcgt ctgaagtatg gcttcaagcc gtttatctgg caggttggcg gcaaatttac
   104161 ctggccgctg gataaagaca acttcgagta ccactatccg cgcggtttcg atgattgctt
   104221 cactattgaa ccggatctgc cgttcaagtc attcctgtaa tctgtcgttt ataccggata
   104281 gcgtggagaa gttcgcgcta tccggcgtag tatttcctgg ctatttcaaa tatcatctaa
   104341 aaaaatcaaa ttttatcgga atagctcatg actgaagcac aaagacatca aatcctcctg
   104401 gaaatgctcg cacaattggg ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt
   104461 tcgcctgcca ctgcgcgacg cgatatcaat aaacttgacg aaagcggcaa actgaaaaag
   104521 gtgcgcaatg gcgcagaagc tattacccag cagcgcccgc gctggacgcc gatgaatctg
   104581 catcaggcgc agaatcacga tgaaaaagta cgtatcgcta aagcagcctc gcagctggtt
   104641 aatccgggcg aaagcgtagt catcaactgc ggctccaccg cgtttctgct tgggcgggaa
   104701 atgtgtggca agccagtgca aatcatcact aattatctac cgctggcaaa ttacctgatc
   104761 gatcaagaac atgacagcgt gatcattatg ggtggacagt acaacaaaag tcagtccatc
   104821 actttaagcc cgcagggcag cgaaaacagt ctctatgccg ggcactggat gtttaccagc
   104881 ggaaaagggc tgaccgcaga agggttgtat aaaaccgata tgctgacagc aatggcagag
   104941 cagaagatgc tgagcgtggt agggaaactg gtggtactgg ttgatagcag taagattggc
   105001 gaacgcgcgg gaatgctttt tagccgtgcc gatcaaatcg atatgctgat caccggcaaa
   105061 aatgctaacc cggaaatcct gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt
   105121 taaagatgct ggcggaaaaa tgtcacggca gcatctaacg cctcaggcgt aatgcggtga
   105181 cgcacgcctg gctgccatga acaggttagc agtttatccc gtcccgtctc gcttaaggcc
   105241 tgctgcaaac gtagtgattc gtcggcaggc acaacgtcat cgtcgaggcc atgccacagc
   105301 agtagaggtc tgtccccaag ttgttccagg tggtttgtcg cttcccactc tgccagcggc
   105361 gcgataatgt tattgaattc attctgctgt gctgccgttt caggtatcag cggtggaaac
   105421 agtgaacggg cgagcgatgt aaaatagccc gatcccatca tgctggcggt acatttcacc
   105481 gtggggtggc gagcggtaat tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact
   105541 gccagacggt catcaagcag ccagttttct tcggctattg ccgcacgtaa agtagtgaat
   105601 tcctgcatac tttgtagcaa gatttgccag aattgattta accgccgcgc ttcgtcgcta
   105661 ctaaaacggc taccgtgatc gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc
   105721 agcgcaacgg caaaatagct atacaccagg ctggatgaag taaagccgtg ataaaaaatt
   105781 acgcacggta acggggtatc tttttgcccg acaggataag catgaagaac gggaatatct
   105841 gccagctcgc gtgattctat ttcaatcatc tgtctctcct ttttgtctgg tatgacatga
   105901 agaaatcctt agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt
   105961 acgctttcgt cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc
   106021 cccaaagtta aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt
   106081 taccggtttg ccagtttatt attggtgttg atgttaagtg cctgtagcgc actgcaaggt
   106141 acgccacagc cagcaccgcc agtaacgaac catccgcaag agattcgccg cgaccagacg
   106201 caagggttac agcgaatagg tagcgtaagc acgatggttc ggggttctcc ggatgacgca
   106261 ttagcagaaa tcagagcaaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg
   106321 gtggacgaaa ccatcgtgac aggacagtgg tattcacaag ccattttgta ccgtaaataa
   106381 atcagacaac ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc
   106441 acaataaatt accggctgga agcctgggcg tttattaccg cgatggaatg ccctgcaaca
   106501 tgtggggaaa cgaaaaatgg agctgacgat gaaacaatta cttgcctcac cctcgctgca
   106561 attagtgact tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg
   106621 cgtgacgggc ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca
   106681 ggacgtggaa cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat
   106741 tattaccatg tacgaagacc agcagcctga taactggcgc gtacaggcga tactgtatgc
   106801 atgagtgtca tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc
   106861 tacaattttc acatatttca ttgagttaat cgaaaccagc gtcgcatcag tcgtatacgc
   106921 ttacacacac actccccccg tcgcccgcag caataaatca ttctggatct gctctgacaa
   106981 ccgtacgccg ccgcgcgtat ctaacatcac ctgacaccac gcctgtgcca ttggcggaga
   107041 ggcatatttc aacatttgcg caccgcagcc cagcaggaat agctgatgag taatctctcg
   107101 ccccaattct tcagctggct tgcgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa
   107161 atagcgatcc tgccctttca cttccacaaa cgcttccgac aataagtcgt atacgcccgc
   107221 ttgcttattg agaacgcgca gcacatccag gcacataata ttgccggaac cttcccaaat
   107281 actgtttacc ggcatctccc ggtaaagccg cggcagctcg ctctcctcgc aataaccaat
   107341 gccgcccagc acctccatcg cttcggccac aaacggcata cctcttttgc agatcacaaa
   107401 ttttgccgca ggcgtaaata aacgcgccca cagggcttct ttggcatcgg cacgccggtc
   107461 ccacgctcgc gcaagacgaa acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg
   107521 acttaagaca tgacgcataa ggggctgttg gatcaatgga ttaccaaaaa catggcgttg
   107581 atgtgcatga taaatcgcca gcgaaaatgc acggcgcatc atggcatggc tacccagggc
   107641 gcaatcaaaa cgcgtcatcc cacccatttt caggatcaga cgaattcctt ccccttcctg
   107701 ccccaacaac cagccaatgg catcctgaaa ctccacttcg cagctggcgt tagagcgatt
   107761 acccagctta tctttcagcc gctcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa
   107821 gcgcggcaca aaaaagcagg acaaaccgcc cgtagtctgc gccagcacca gatgcgcatc
   107881 gctttgcggc accgagaaaa accatttatg ccccaccagc cgataagagc catcttccag
   107941 gcg
//
LOCUS       DAHBHC010000006       106701 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHBHC010000006 DAHBHC010000000
VERSION     DAHBHC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 106701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.006"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..141)
                     /locus_tag="M2A35_000547"
     CDS             complement(<1..141)
                     /locus_tag="M2A35_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCB7196892.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLTERIGVAAAARELSLYES
                     QLY"
     gene            complement(242..1060)
                     /locus_tag="M2A35_000548"
     CDS             complement(242..1060)
                     /locus_tag="M2A35_000548"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7196893.1"
                     /translation="MINSIKHHSQRKLKIVNIRTSKYIILYTNNCTINFSLSNKKLTC
                     GFNQFIFIDKGTCFSAYLKKNDEHKEPYVAIRFNEDKLQTLRRVIAEIYFKGHQVINQ
                     SAISRDKILTLTPTDELILFFKKLATTNDEKLILIKTLLFISKFKDRDYIIRSLLTSS
                     VSHFSDKVRAFIEMNIKRKWCVRDIAKHFHLSESAVRKRLAKENLTISGMALQIRLNR
                     AMDLIIENNLQISQISSEVGMSSPSYFIKKFREYFGITPKKFKHYFITYPKIIL"
     gene            complement(1969..2169)
                     /locus_tag="M2A35_000549"
     CDS             complement(1969..2169)
                     /locus_tag="M2A35_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCB7196894.1"
                     /translation="MRIDICIAKEKMTKMPNSAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDELSVTRTADKDSAKTFVQ"
     gene            complement(2231..3328)
                     /locus_tag="M2A35_000550"
     CDS             complement(2231..3328)
                     /locus_tag="M2A35_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7196895.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGAQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQQQPQKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQRSRNKTS"
     gene            3417..4454
                     /gene="dusA"
                     /locus_tag="M2A35_000551"
     CDS             3417..4454
                     /gene="dusA"
                     /locus_tag="M2A35_000551"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCB7196896.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            4588..4830
                     /gene="pspG"
                     /locus_tag="M2A35_000552"
     CDS             4588..4830
                     /gene="pspG"
                     /locus_tag="M2A35_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCB7196897.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(4996..5979)
                     /locus_tag="M2A35_000553"
     CDS             complement(4996..5979)
                     /locus_tag="M2A35_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCB7196898.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            6062..7477
                     /gene="dnaB"
                     /locus_tag="M2A35_000554"
     CDS             6062..7477
                     /gene="dnaB"
                     /locus_tag="M2A35_000554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCB7196899.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            7530..8609
                     /gene="alr"
                     /locus_tag="M2A35_000555"
     CDS             7530..8609
                     /gene="alr"
                     /locus_tag="M2A35_000555"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCB7196900.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGIIKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            8862..10055
                     /gene="tyrB"
                     /locus_tag="M2A35_000556"
     CDS             8862..10055
                     /gene="tyrB"
                     /locus_tag="M2A35_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCB7196901.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPMGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10277..10780)
                     /locus_tag="M2A35_000557"
     CDS             complement(10277..10780)
                     /locus_tag="M2A35_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196902.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLCCFAEEVKGDYS
                     HYRTFLFDIGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEKFYR
                     NKPDTYY"
     gene            11182..11895
                     /gene="aphA"
                     /locus_tag="M2A35_000558"
     CDS             11182..11895
                     /gene="aphA"
                     /locus_tag="M2A35_000558"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCB7196903.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            12006..12422
                     /locus_tag="M2A35_000559"
     CDS             12006..12422
                     /locus_tag="M2A35_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCB7196904.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHEYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            12426..12782
                     /locus_tag="M2A35_000560"
     CDS             12426..12782
                     /locus_tag="M2A35_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCB7196905.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12817..15639)
                     /gene="uvrA"
                     /locus_tag="M2A35_000561"
     CDS             complement(12817..15639)
                     /gene="uvrA"
                     /locus_tag="M2A35_000561"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCB7196906.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            15893..16459
                     /gene="ssb1"
                     /locus_tag="M2A35_000562"
     CDS             15893..16459
                     /gene="ssb1"
                     /locus_tag="M2A35_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCB7196907.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGWGQPQQPQGGNQFSG
                     GAQSRPQQSAPAAPSNEPPMDFDDDIPF"
     gene            complement(16558..16839)
                     /locus_tag="M2A35_000563"
     CDS             complement(16558..16839)
                     /locus_tag="M2A35_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCB7196908.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            17269..18855
                     /gene="pdeC"
                     /locus_tag="M2A35_000564"
     CDS             17269..18855
                     /gene="pdeC"
                     /locus_tag="M2A35_000564"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCB7196909.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIDLAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(18858..19181)
                     /gene="soxS"
                     /locus_tag="M2A35_000565"
     CDS             complement(18858..19181)
                     /gene="soxS"
                     /locus_tag="M2A35_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCB7196910.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            19267..19731
                     /gene="soxR"
                     /locus_tag="M2A35_000566"
     CDS             19267..19731
                     /gene="soxR"
                     /locus_tag="M2A35_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCB7196911.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            20277..21626
                     /gene="ghxP"
                     /locus_tag="M2A35_000567"
                     /gene_synonym="yjcD"
     CDS             20277..21626
                     /gene="ghxP"
                     /locus_tag="M2A35_000567"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCB7196912.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            21777..23426
                     /locus_tag="M2A35_000568"
     CDS             21777..23426
                     /locus_tag="M2A35_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCB7196913.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23579..24871)
                     /locus_tag="M2A35_000569"
     CDS             complement(23579..24871)
                     /locus_tag="M2A35_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCB7196914.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSVTSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASYANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIDIPQAENGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNSIFQDCNMYRTNFYCAIMEKILFDNCILDDSYFAHVKMTDGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMAKVDF
                     NDANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAAAMKITADIMQHAD"
     gene            complement(25049..26698)
                     /gene="actP"
                     /locus_tag="M2A35_000570"
     CDS             complement(25049..26698)
                     /gene="actP"
                     /locus_tag="M2A35_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCB7196915.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(26695..27009)
                     /locus_tag="M2A35_000571"
     CDS             complement(26695..27009)
                     /locus_tag="M2A35_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCB7196916.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(27209..29167)
                     /gene="acs"
                     /locus_tag="M2A35_000572"
     CDS             complement(27209..29167)
                     /gene="acs"
                     /locus_tag="M2A35_000572"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCB7196917.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            29559..30995
                     /gene="nrfA"
                     /locus_tag="M2A35_000573"
     CDS             29559..30995
                     /gene="nrfA"
                     /locus_tag="M2A35_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCB7196918.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            31040..31606
                     /gene="nrfB"
                     /locus_tag="M2A35_000574"
     CDS             31040..31606
                     /gene="nrfB"
                     /locus_tag="M2A35_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCB7196919.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            31603..32274
                     /gene="nrfC"
                     /locus_tag="M2A35_000575"
     CDS             31603..32274
                     /gene="nrfC"
                     /locus_tag="M2A35_000575"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCB7196920.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            32271..33227
                     /gene="nrfD"
                     /locus_tag="M2A35_000576"
     CDS             32271..33227
                     /gene="nrfD"
                     /locus_tag="M2A35_000576"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCB7196921.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            33247..34965
                     /locus_tag="M2A35_000577"
     CDS             33247..34965
                     /locus_tag="M2A35_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCB7196922.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            34958..35341
                     /gene="nrfF"
                     /locus_tag="M2A35_000578"
     CDS             34958..35341
                     /gene="nrfF"
                     /locus_tag="M2A35_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCB7196923.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            35338..35934
                     /gene="nrfG"
                     /locus_tag="M2A35_000579"
     CDS             35338..35934
                     /gene="nrfG"
                     /locus_tag="M2A35_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCB7196924.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            36276..37589
                     /gene="gltP"
                     /locus_tag="M2A35_000580"
     CDS             36276..37589
                     /gene="gltP"
                     /locus_tag="M2A35_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCB7196925.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(37667..38356)
                     /locus_tag="M2A35_000581"
     CDS             complement(37667..38356)
                     /locus_tag="M2A35_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCB7196926.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(38450..40597)
                     /gene="fdhF"
                     /locus_tag="M2A35_000582"
     CDS             complement(38450..40597)
                     /gene="fdhF"
                     /locus_tag="M2A35_000582"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(40178..40180),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCB7196927.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(40795..42261)
                     /gene="mdtP"
                     /locus_tag="M2A35_000583"
     CDS             complement(40795..42261)
                     /gene="mdtP"
                     /locus_tag="M2A35_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCB7196928.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(42258..44309)
                     /gene="mdtO"
                     /locus_tag="M2A35_000584"
     CDS             complement(42258..44309)
                     /gene="mdtO"
                     /locus_tag="M2A35_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCB7196929.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATALEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(44309..45340)
                     /gene="mdtN"
                     /locus_tag="M2A35_000585"
     CDS             complement(44309..45340)
                     /gene="mdtN"
                     /locus_tag="M2A35_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCB7196930.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(45359..45634)
                     /locus_tag="M2A35_000586"
     CDS             complement(45359..45634)
                     /locus_tag="M2A35_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196931.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(45843..47829)
                     /gene="yjcS"
                     /locus_tag="M2A35_000587"
                     /pseudo
     CDS             complement(45843..47829)
                     /gene="yjcS"
                     /locus_tag="M2A35_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
     gene            complement(48102..49031)
                     /gene="alsK"
                     /locus_tag="M2A35_000588"
     CDS             complement(48102..49031)
                     /gene="alsK"
                     /locus_tag="M2A35_000588"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCB7196932.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(49015..49710)
                     /gene="alsE"
                     /locus_tag="M2A35_000589"
     CDS             complement(49015..49710)
                     /gene="alsE"
                     /locus_tag="M2A35_000589"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCB7196933.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(49721..50701)
                     /gene="alsC"
                     /locus_tag="M2A35_000590"
     CDS             complement(49721..50701)
                     /gene="alsC"
                     /locus_tag="M2A35_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCB7196934.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(50680..52211)
                     /gene="alsA"
                     /locus_tag="M2A35_000591"
                     /pseudo
     CDS             complement(50680..52211)
                     /gene="alsA"
                     /locus_tag="M2A35_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            complement(52338..53273)
                     /gene="alsB"
                     /locus_tag="M2A35_000592"
     CDS             complement(52338..53273)
                     /gene="alsB"
                     /locus_tag="M2A35_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCB7196935.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(53332..54222)
                     /gene="alsR"
                     /locus_tag="M2A35_000593"
                     /gene_synonym="rpiR"
     CDS             complement(53332..54222)
                     /gene="alsR"
                     /locus_tag="M2A35_000593"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCB7196936.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALSVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            54581..55030
                     /gene="rpiB"
                     /locus_tag="M2A35_000594"
     CDS             54581..55030
                     /gene="rpiB"
                     /locus_tag="M2A35_000594"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCB7196937.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
     gene            55099..55428
                     /gene="yjdP"
                     /locus_tag="M2A35_000595"
     CDS             55099..55428
                     /gene="yjdP"
                     /locus_tag="M2A35_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCB7196938.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(55575..56333)
                     /gene="phnP"
                     /locus_tag="M2A35_000596"
     CDS             complement(55575..56333)
                     /gene="phnP"
                     /locus_tag="M2A35_000596"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCB7196939.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDALFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(56335..56769)
                     /gene="phnO"
                     /locus_tag="M2A35_000597"
     CDS             complement(56335..56769)
                     /gene="phnO"
                     /locus_tag="M2A35_000597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCB7196940.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(56756..57313)
                     /gene="phnN"
                     /locus_tag="M2A35_000598"
                     /pseudo
     CDS             complement(56756..57313)
                     /gene="phnN"
                     /locus_tag="M2A35_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
     gene            complement(57313..58449)
                     /gene="phnM"
                     /locus_tag="M2A35_000599"
     CDS             complement(57313..58449)
                     /gene="phnM"
                     /locus_tag="M2A35_000599"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCB7196941.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAL
                     CRVRQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(58446..59126)
                     /gene="phnL"
                     /locus_tag="M2A35_000600"
     CDS             complement(58446..59126)
                     /gene="phnL"
                     /locus_tag="M2A35_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCB7196942.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(59237..59995)
                     /gene="phnK"
                     /locus_tag="M2A35_000601"
     CDS             complement(59237..59995)
                     /gene="phnK"
                     /locus_tag="M2A35_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCB7196943.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     SGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(59992..60837)
                     /gene="phnJ"
                     /locus_tag="M2A35_000602"
     CDS             complement(59992..60837)
                     /gene="phnJ"
                     /locus_tag="M2A35_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCB7196944.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(60830..61894)
                     /gene="phnI"
                     /locus_tag="M2A35_000603"
     CDS             complement(60830..61894)
                     /gene="phnI"
                     /locus_tag="M2A35_000603"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCB7196945.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(61894..62478)
                     /gene="phnH"
                     /locus_tag="M2A35_000604"
     CDS             complement(61894..62478)
                     /gene="phnH"
                     /locus_tag="M2A35_000604"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCB7196946.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(62475..62840)
                     /gene="phnG"
                     /locus_tag="M2A35_000605"
     CDS             complement(62475..62840)
                     /gene="phnG"
                     /locus_tag="M2A35_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCB7196947.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAVR
                     LTDGTLGYSWVLGRDKQHAERCALIDALMQQSRYFQNLSETLIAPLDADRMARIAARQ
                     AEVNASRVDFFTMVRGDNA"
     gene            complement(62841..63566)
                     /gene="phnF"
                     /locus_tag="M2A35_000606"
     CDS             complement(62841..63566)
                     /gene="phnF"
                     /locus_tag="M2A35_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCB7196948.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(63587..64366)
                     /gene="phnE"
                     /locus_tag="M2A35_000607"
     CDS             complement(63587..64366)
                     /gene="phnE"
                     /locus_tag="M2A35_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCB7196949.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(64472..65488)
                     /gene="phnD"
                     /locus_tag="M2A35_000608"
     CDS             complement(64472..65488)
                     /gene="phnD"
                     /locus_tag="M2A35_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7196950.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(65513..66301)
                     /gene="phnC"
                     /locus_tag="M2A35_000609"
     CDS             complement(65513..66301)
                     /gene="phnC"
                     /locus_tag="M2A35_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCB7196951.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFIWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(66434..66877)
                     /gene="yjdN"
                     /locus_tag="M2A35_000610"
     CDS             complement(66434..66877)
                     /gene="yjdN"
                     /locus_tag="M2A35_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCB7196952.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(67037..67372)
                     /locus_tag="M2A35_000611"
     CDS             complement(67037..67372)
                     /locus_tag="M2A35_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCB7196953.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            67773..70001
                     /gene="crfC"
                     /locus_tag="M2A35_000612"
     CDS             67773..70001
                     /gene="crfC"
                     /locus_tag="M2A35_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCB7196954.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            69998..70876
                     /locus_tag="M2A35_000613"
     CDS             69998..70876
                     /locus_tag="M2A35_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCB7196955.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRKHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            71140..72642
                     /gene="proP"
                     /locus_tag="M2A35_000614"
     CDS             71140..72642
                     /gene="proP"
                     /locus_tag="M2A35_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCB7196956.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            72754..72843
                     /gene="pmrR"
                     /locus_tag="M2A35_000615"
     CDS             72754..72843
                     /gene="pmrR"
                     /locus_tag="M2A35_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCB7196957.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(72819..73919)
                     /gene="pmrB"
                     /locus_tag="M2A35_000616"
     CDS             complement(72819..73919)
                     /gene="pmrB"
                     /locus_tag="M2A35_000616"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCB7196958.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITIQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(73920..74588)
                     /gene="basR"
                     /locus_tag="M2A35_000617"
     CDS             complement(73920..74588)
                     /gene="basR"
                     /locus_tag="M2A35_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCB7196959.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(74585..76228)
                     /gene="eptA"
                     /locus_tag="M2A35_000618"
     CDS             complement(74585..76228)
                     /gene="eptA"
                     /locus_tag="M2A35_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCB7196960.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(76332..77669)
                     /gene="adiC"
                     /locus_tag="M2A35_000619"
     CDS             complement(76332..77669)
                     /gene="adiC"
                     /locus_tag="M2A35_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCB7196961.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(77806..78567)
                     /gene="adiY"
                     /locus_tag="M2A35_000620"
     CDS             complement(77806..78567)
                     /gene="adiY"
                     /locus_tag="M2A35_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCB7196962.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(78892..81159)
                     /gene="adiA"
                     /locus_tag="M2A35_000621"
     CDS             complement(78892..81159)
                     /gene="adiA"
                     /locus_tag="M2A35_000621"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCB7196963.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(81358..82266)
                     /gene="melR"
                     /locus_tag="M2A35_000622"
     CDS             complement(81358..82266)
                     /gene="melR"
                     /locus_tag="M2A35_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCB7196964.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            82549..83904
                     /gene="melA"
                     /locus_tag="M2A35_000623"
     CDS             82549..83904
                     /gene="melA"
                     /locus_tag="M2A35_000623"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCB7196965.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            84007..85428
                     /gene="melB"
                     /locus_tag="M2A35_000624"
     CDS             84007..85428
                     /gene="melB"
                     /locus_tag="M2A35_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCB7196966.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(85567..86196)
                     /locus_tag="M2A35_000625"
     CDS             complement(85567..86196)
                     /locus_tag="M2A35_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCB7196967.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(86319..87965)
                     /gene="fumB"
                     /locus_tag="M2A35_000626"
     CDS             complement(86319..87965)
                     /gene="fumB"
                     /locus_tag="M2A35_000626"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCB7196968.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(88043..89383)
                     /gene="dcuB"
                     /locus_tag="M2A35_000627"
     CDS             complement(88043..89383)
                     /gene="dcuB"
                     /locus_tag="M2A35_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCB7196969.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(89954..90673)
                     /gene="dcuR"
                     /locus_tag="M2A35_000628"
     CDS             complement(89954..90673)
                     /gene="dcuR"
                     /locus_tag="M2A35_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCB7196970.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(90670..92301)
                     /gene="dcuS"
                     /locus_tag="M2A35_000629"
     CDS             complement(90670..92301)
                     /gene="dcuS"
                     /locus_tag="M2A35_000629"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCB7196971.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            92482..92712
                     /locus_tag="M2A35_000630"
     CDS             92482..92712
                     /locus_tag="M2A35_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCB7196972.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            92724..92996
                     /locus_tag="M2A35_000631"
     CDS             92724..92996
                     /locus_tag="M2A35_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7196973.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            93223..93519
                     /gene="ghoS"
                     /locus_tag="M2A35_000632"
     CDS             93223..93519
                     /gene="ghoS"
                     /locus_tag="M2A35_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCB7196974.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            93547..93720
                     /gene="ghoT"
                     /locus_tag="M2A35_000633"
     CDS             93547..93720
                     /gene="ghoT"
                     /locus_tag="M2A35_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCB7196975.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(93839..95356)
                     /gene="lysS"
                     /locus_tag="M2A35_000634"
     CDS             complement(93839..95356)
                     /gene="lysS"
                     /locus_tag="M2A35_000634"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCB7196976.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(95593..97050)
                     /gene="dtpC"
                     /locus_tag="M2A35_000635"
     CDS             complement(95593..97050)
                     /gene="dtpC"
                     /locus_tag="M2A35_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCB7196977.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(97109..99256)
                     /gene="cadA"
                     /locus_tag="M2A35_000636"
     CDS             complement(97109..99256)
                     /gene="cadA"
                     /locus_tag="M2A35_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCB7196978.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(99336..100670)
                     /gene="cadB"
                     /locus_tag="M2A35_000637"
     CDS             complement(99336..100670)
                     /gene="cadB"
                     /locus_tag="M2A35_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCB7196979.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(101036..102574)
                     /gene="cadC"
                     /locus_tag="M2A35_000638"
     CDS             complement(101036..102574)
                     /gene="cadC"
                     /locus_tag="M2A35_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCB7196980.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(103385..104227)
                     /locus_tag="M2A35_000639"
     CDS             complement(103385..104227)
                     /locus_tag="M2A35_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCB7196981.1"
                     /translation="MSDITISRPELVNRHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTAPAWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYQSLEYDNFPEISEANILSTFEQLHQNKDEVFERGMINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAM"
     gene            complement(104312..104509)
                     /locus_tag="M2A35_000640"
     CDS             complement(104312..104509)
                     /locus_tag="M2A35_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCB7196982.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCELGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            complement(104521..104955)
                     /locus_tag="M2A35_000641"
     CDS             complement(104521..104955)
                     /locus_tag="M2A35_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196983.1"
                     /translation="MGRIVVDVDGIELAELINVVCDNGHSLRVVDESDRASTDCTPSF
                     AALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFTGSGYLLRTDAWSYPVLRL
                     KRQGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTFDW"
     gene            complement(105056..105382)
                     /locus_tag="M2A35_000642"
     CDS             complement(105056..105382)
                     /locus_tag="M2A35_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCB7196984.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDN"
     gene            complement(105472..105840)
                     /locus_tag="M2A35_000643"
     CDS             complement(105472..105840)
                     /locus_tag="M2A35_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCB7196985.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            complement(106003..106224)
                     /locus_tag="M2A35_000644"
     CDS             complement(106003..106224)
                     /locus_tag="M2A35_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCB7196986.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAHTCREVQ
                     DIPGVLAVFAERRKDSFGLYVRLMSVTLN"
     gene            complement(106287..>106701)
                     /locus_tag="M2A35_000645"
     CDS             complement(106287..>106701)
                     /locus_tag="M2A35_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCB7196987.1"
                     /translation="ALKTLDRHLHEPGVAFTSTHAAREWLILNMAGLEREEFRVLYLN
                     NQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLI
                     TERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
BASE COUNT        25369 a        26477 c        27831 g        27024 t
ORIGIN      
        1 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
       61 agtgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
      121 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
      181 gtccactatt ggtgggtaag atcattctga tatcagtcca aagcaaatgc atcagtgtaa
      241 ttcagagaat gattttggga tatgtaatga agtaatgctt aaactttttt ggagttattc
      301 caaaatattc cctaaatttt tttatgaagt atgatggact cgacattcct acttccgatg
      361 atatctgaga tatctggaga ttgttttcga ttataagatc catagctcta tttaatctta
      421 tttgtaaagc cattccgctg atggtcaaat tttcttttgc aagtcgtttt cttactgcag
      481 actcagatag gtgaaaatgc ttagctatat cccgaacgca ccattttctc tttatgttca
      541 tttcaataaa tgctcttacc ttatcactaa agtgactgac agatgatgta agcaacgaac
      601 gaatgatgta atcacgatct ttgaattttg agataaaaag tagcgtctta atcaaaatta
      661 gtttttcatc attggtagtt gcaagttttt tgaaaaagag tataagctca tctgtaggcg
      721 taagtgtcag tattttatct cttgatattg ctgattgatt aattacctga tgacctttaa
      781 agtagatttc tgcaattact cgtcggagtg tttgtagttt atcctcgttg aatcttattg
      841 ccacatatgg ttctttatgt tcatcattct ttttcaggta ggcagaaaaa caagttcctt
      901 tatcaatgaa aatgaattgg ttaaacccgc atgtcagttt tttatttgat agtgagaaat
      961 taatagtgca attatttgta taaagaatga tatatttact ggttcttatg ttaacgatct
     1021 ttaactttct ttgtgaatga tgtttgatgc tattaatcat gtgtttagcc atttttttgg
     1081 ggtgtggtaa ttataactat caacttcctc aatggaatat cttggcatta aaaataggat
     1141 gtttttatta aattttggcc gctgtggtta aagtaaagga tgagttaata tccagtccat
     1201 ccttaatggg agtgattagt tcaccatcag ataaatcgta agtggtgttt ttgggggctc
     1261 gtcaagctca aaaatggccg ggagaaaatt ggatataact acagggtagg acttacgaaa
     1321 gtgacagagt tgctatagga atataaaaac attcatggtg aactggggtc tgaaaagtca
     1381 catgccctca ttagaaatgg tagcatttta agtgtgggaa tgaaaaacag aagagcaaaa
     1441 atcatccata atatgtagaa ttagatcatg tcaacccata tttagtcctt gttcgatagc
     1501 aagcctcatt gacttcgccg ttatcaccag atatggcgag ttctttgtaa gtgggagagc
     1561 tatttttaga tgtcgtgcag cagacatttt tgccgagcgc ttcatccgga gcgatgaacc
     1621 gtagattttt ctggttggag ccgatcatat gtgatgctat ggggatattt tttcagtagg
     1681 ctaggtggga attgtaccgt taatattata ggcttctacc cagtaaaaag aggcgaggaa
     1741 acatgcacga caggtgctac acgtcatgca tagattggga ttactcatga ctagccacag
     1801 ctataggtcg ttaagagatt cgcagcacgc tgtatgcaat aacgtgctgc ggctggctgg
     1861 tgaactttcg atagtgcgag tattgaatga tttccagccg ttaccgattt tacgtgttta
     1921 ttattgaaca aaccactcgt cagcagactc cctggtatct ttcagagtct actgaacaaa
     1981 tgtttttgca gaatccttat ctgcggtgcg tgtaacagaa agctcatcat tgctggtggc
     2041 ttttacgatc acttctacat cgtcataacg cttactgatg cgccgagtta attcttcctt
     2101 taacgcatcc acagcactgt ttggcatttt agtcattttc tctttggcta tgcagatatc
     2161 aatacgcata aaaatccctc cacgctgtgt ttatatacag tgttattttt aactgtgtaa
     2221 ataaacagta tcaagaggtc ttgtttctgc tcctttggag ttcttcaaaa cgattatgta
     2281 aagatttcgg atacagttcg gtatatacct gccatagcac gtttaatgaa cgatgccctg
     2341 taacctgagc gacttcctca atactaaaac cagcctcaaa taagcgactt gccccttccc
     2401 tacgcaaatc atggtatcgc agatctttaa tacctaattt gcttcttacc ctctgaaatc
     2461 ctgcggtaac agaagtgctg ttatatggaa aaatgaattc tgatttttgg ggttgttgct
     2521 ggacgatatc ccaggcttcc ccaagcaagg caactttcat atggttgcct tcctttttac
     2581 gtggatcttt cctatctctt actagtatag atttttgttc ctggtcgaga tcttcccatc
     2641 gtaaccggca tacttctcca attcgcatac aggaccacac agaaaatttg aggatatcaa
     2701 cgaacggaat ttttgagcat ttatgagtag atcgttgttg aaggccttca atgagcatgt
     2761 ccagttcatc agatgctggt ctacgattac gacggtttga tttaccaatc aaaccgagtt
     2821 taagtagata tggacgagcg cttttcgccg ggtttgatgt gtaattgatt ccgtatacag
     2881 gttttgccgc atccagaaca ctgccaagat aactaacatc gtggctgact gttgctgggc
     2941 ctgcaccagc gttgtttctt agcctgcaat gttcaattac gtcattttct gtcagttcag
     3001 atagtttgat cgcggagatg tcgctatcca taagcagttc cagcacatat cttttagtac
     3061 ggcctgcttt acctccggca tttgggtcat ttaaatattt gtgtagtaag tcacggactg
     3121 taagtccgtc aacagcattt gatgatggaa tgccatatag atctaattcc atcactttct
     3181 gtgcgcccca tgttttggca tgagcatgtt tagggaatgt tttgctttcc ctgtaagtga
     3241 taacaccttt ttctttgata atcacattac agcgatagcg tggtgtgcca tcggatttta
     3301 gtcgtttctc tatgttatag tacgccatta cacgacctcg ttatttcggg ttcccataaa
     3361 acgtgggaac ctatgcggga acctaatgcg agaaaaatag cctgaaatgt tcaaaaatgc
     3421 acgataatca tgaaacacaa aaaattaatc aaaccagcgt gatgccggaa aaaactggtg
     3481 tttactggaa ttctcggttt agcattgctc ctatgctcga ctggacggac agacattgcc
     3541 gctatttctt gcgtctgctt tcccgcaata cgttgctgta taccgaaatg gtgaccacag
     3601 gggcgattat tcacggtaaa ggtgattatc tggcgtacag tgaagaagaa catccggtag
     3661 cgttgcaact gggcggtagc gatccggcgg cgctggcgca gtgtgcaaag ctggcagaag
     3721 cgcgcggata tgatgagatc aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg
     3781 gcatgtttgg tgcgtgtctg atgggtaatg cgcagctggt tgccgactgt gtgaaagcga
     3841 tgcgcgatgt ggtgtcgatt ccagtgacgg tgaaaacgcg tattggcatc gatgaccagg
     3901 acagctatga atttctctgc gatttcatca acactgtttc cggcaaaggc gagtgtgaga
     3961 tgtttatcat ccacgcacgt aaagcctggc tttcggggtt aagcccgaaa gaaaaccgtg
     4021 aaatcccgcc gctcgattat ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga
     4081 caatgtcgat taacggtggt atcaagtcgc tggaagaggc caaagcacac ctgcaacata
     4141 tggatggcgt gatggtcggg cgcgaggcgt atcagaatcc gggtattctg gcggcagtag
     4201 accgggagat ctttggttcc tcggataccg atgccgatcc ggtggcggta gtgcgcgcca
     4261 tgtatccgta cattgagcgt gaactcagcc aggggacgta tctcggccat attacccggc
     4321 atatgttggg cttgttccag ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg
     4381 aaaatgccca taaagcgggt gcagacatta atgtgctgga acacgcgctc aaactggtgg
     4441 cggataagcg ttaacttttc accaaaaagt agtcaaattc accacgccct gcgcaccgtc
     4501 gcggggcgtt ttgctgttaa atcaatagat tatttttggc atgattcttg taatgccagc
     4561 aagagatttc atatttggga gagcatcatg ctggaactac tttttgtgat tggctttttt
     4621 gtcatgctga tggtcaccgg cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg
     4681 acggccatta tgttcctcgg cggtatgctg gcattgatga ttaagttgct gccgtggtta
     4741 ctactggcga ttgcggtggt gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa
     4801 tatcagcgtt atgaccgctg gcgttactaa gggattgtgc ggatgatcac aacctaaggt
     4861 tttatcctta gaacaaaata ggaattgata atcaaatctg tcactattgc gcctctaaca
     4921 gattcatcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg
     4981 gaagccctat ttcttttatg gaatcaacag gctggaacct tgcgtcgccc ggctttccag
     5041 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac
     5101 cttaatcaca ccgctggcaa ttaaagagaa cagttcatta ctggcctcgg ttaattcctc
     5161 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt
     5221 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa
     5281 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta
     5341 caccacgcgc actttcttac cgccggtgat ctcttttaac cgctcgacca gatcctcttc
     5401 acgatagtta ataacctgcc acgcgcccgc ttttagcgcg ctctgcgctt tttgcgcggt
     5461 tcctacggtg ccgataagtt tcgcgcccag ggcttttgcc cactggcagg caatcaagcc
     5521 aacgccgcca gctgctgcgt ggaacaggaa ctgctcatcg ggtttaattt cataggtttt
     5581 gcgcagcaga taataaaccg ttaagccttt caggaaagat gctgcagctt gctcaaaaga
     5641 aattgccgca ggcagaatcg ccgctttatc cgcattaatg ttatgcacag agctgtaagc
     5701 gcctaacgcc gactgcgcat agactacacg atcgcctgcc ttaatatgct ttacaccact
     5761 gccgacttta ctcacgatgc ctgccgcttc ggtacctaat ccgctgggta gtgatggcgg
     5821 cgggtaaagg ccgctgcgga tatacgtgtc gataaaattg atgccgatgg ctttattttc
     5881 gacctggatt tcattctctg ccggatcggc aggagtgaac tctacggctt gaagtacttc
     5941 cgggccaccg tgcttgtgaa attcaattcg tgttgccatg tgtcctccag aacgtatcgt
     6001 cagggtctgc ttcatatggt aaagtttcga cccattcttt atctcggtaa ctccattcac
     6061 tatggcagga aataaaccct tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca
     6121 agttgccggg ctgaaagtgc ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg
     6181 tttaatgctg gataacgaac gctgggacga tgtagccgag cgtgtggtgg cagacgattt
     6241 ttacacccgc ccacaccgcc atatctttac tgaaatggcg cgtttgcagg aaagcggtag
     6301 tcctatcgat ctgattaccc ttgcggaatc gctggaacgc caggggcaac tcgatagcgt
     6361 cggtggtttt gcttatctgg cagagctgtc aaaaaatacg ccaagtgcgg cgaacatcag
     6421 tgcttatgct gacatcgtgc gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa
     6481 tgagattgct gaagccggtt ttgatccgca ggggcgtacc agcgaagatc tgctggacct
     6541 tgctgaatcc cgcgtcttta aaattgccga aagtcgtgca aacaaagacg aagggccgaa
     6601 gaacatcgcc gatgtgctcg acgcaaccgt ggcgcgtatt gagcagttgt ttcagcagcc
     6661 acacgatggc gttaccggag taaacaccgg ttatgacgat ctcaacaaaa aaaccgctgg
     6721 cttgcagccg tcggatttga tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt
     6781 tgcgatgaac ctcgtcgaaa acgcggcgat gttgcaggat aaaccagtac ttatcttctc
     6841 gctggagatg ccatcagaac agatcatgat gcgttctctg gcgtcgctgt cgcgcgttga
     6901 ccagactaaa atccgtaccg ggcagctcga tgatgaagac tgggcgcgca tttccggcac
     6961 catgggtatt ttgctcgaaa aacgcaatat ttatatcgat gactcctccg gcctgacgcc
     7021 aacggaagtg cgttcccgcg cacgccgtat tgcccgtgaa cacggcggca tcgggcttat
     7081 catgatcgac tacctgcaac tgatgcgcgt accggcgctt tccgataacc gtacgctgga
     7141 aattgcagaa atctctcgct cgctgaaagc actggcgaaa gaactgaacg tgccggtggt
     7201 ggcgctgtcc cagttgaacc gttctctgga acaacgtgcc gacaaacgcc cggtcaactc
     7261 cgacctgcgt gaatctggct ctatcgagca ggatgcggac ttgatcatgt ttatctatcg
     7321 tgatgaggtg tatcacgaaa acagtgattt aaaaggcatc gcggaaatta ttatcggtaa
     7381 acaacgtaac ggcccaatcg ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt
     7441 cgacaactat gcggggccgc agtacgacga cgaataataa ttattttatg aattaggtaa
     7501 ttaaagcaaa cacttatcaa ggaacacaaa tgcaagcggc aactgttgtg attaaccgcc
     7561 gcgctctgcg acacaacctg caacgtcttc gtgaactggc ccctgccagt aaaatggttg
     7621 cggtggtgaa agcgaacgct tatggtcacg gtcttcttga gaccgcgcga acgctccccg
     7681 atgctgacgc ctttggcgta gcccgtctcg aagaagctct gcgactgcgt gcggggggaa
     7741 tcatcaaacc tgtactgtta ctcgaaggct tttttgatgc cagagatctg ccgacgattt
     7801 ctgcgcaaca ttttcatacc gccgtgcata acgaagaaca gctggctgcg ctggaagagg
     7861 ctagcctgga cgagccggtt accgtctgga tgaaactcga taccggtatg caccgtctgg
     7921 gcgtaaggcc ggaacaggct gaggcgtttt atcatcgcct gacccagtgc aaaaacgttc
     7981 gtcagccggt gaatatcgtc agccattttg cgcgcgcgga tgaaccaaaa tgtggcgcaa
     8041 ccgagaaaca actcgctatc tttaatacct tttgcgaagg caaacctggt caacgttcca
     8101 ttgccgcatc gggtggcatt ctgctgtggc cacagtcgca ttttgactgg gtgcgcccgg
     8161 gcatcattct ttatggcgtc tcgccgctgg aagatcgctc caccggtgcc gattttggct
     8221 gtcagccagt gatgtcacta acctccagcc tgattgccgt gcgtgagcat aaagccggag
     8281 aacctgttgg ttatggtgga acctgggtaa gcgaacgtga tacccgtctt ggcgtagtcg
     8341 cgatgggcta tggcgatggt tatccgcgcg ccgcgccgtc cggtacgcca gtgctggtga
     8401 acggtcgcga agtaccgatt gtcgggcgcg tggcgatgga tatgatctgc gtagacttag
     8461 gtccacaagc gcaggacaaa gccggggatc cggtcatttt atggggcgaa ggtttgcccg
     8521 tagaacgtat cgctgaaatg acgaaagtaa gcgcttacga acttattacg cgcctgactt
     8581 caagggtcgc gatgaaatac gtggattaat cgttctgtaa tatttgattg tctgtgccgg
     8641 atgcggcgtg aatgccttat ccggccaata aaatcctaaa aattcaataa gttgatgttc
     8701 tttcatgctc ttataaaggt cgtgcctctg gcggatgtac gtttgtcatg agtctcactc
     8761 tgttgctaat tgccgttcgc tcctgaacat ccactcgatc ttcgccttct tccggtttat
     8821 tgtgttttaa ccacctgccc gtaaacctgg agaaccatcg cgtgtttcaa aaagttgacg
     8881 cctacgctgg cgacccgatt cttacgctta tggagcgttt taaagaagac cctcgcagcg
     8941 acaaagtgaa tttaagtatc ggtctgtact acaacgaaga cggaattatt ccacaactgc
     9001 aagccgtggc ggaggcggaa gcgcgcctga atgcgcagcc tcatggcgct tcgctttatt
     9061 taccgatgga agggcttaac agctatcgcc atgccattgc gccgctgctg tttggtgcgg
     9121 accatccagt actgcaacaa cagcgcgtag caaccattca aacccttggc ggctcagggg
     9181 cattgaaagt gggcgcggat ttcctgaaac gctacttccc ggaatcaggc gtctgggtca
     9241 gcgatcctac ctgggaaaac cacgtagcaa tattcgccgg ggctggattc gaagtgagta
     9301 cttacccctg gtatgacgaa gcgactaacg gcgtgcgctt taatgacctg ttggcgacgc
     9361 tgaaaacatt acctgcccgc agtattgtgt tgctgcatcc atgttgccac aacccaatgg
     9421 gtgccgatct cactaatgat cagtgggatg cggtgattga aattctcaaa gcccgcgagc
     9481 ttattccatt cctcgatatt gcctatcaag gatttggtgc cggtatggaa gaggatgcct
     9541 acgctattcg cgccattgcc agcgctggat tacccgctct ggtgagcaat tcgttctcga
     9601 aaattttctc cctttacggc gagcgcgtcg gcggactttc tgttctgtgt gaagatgccg
     9661 aagccgctgg ccgcgtactg gggcaattga aagcaacagt tcgccgcaac tactccagcc
     9721 cgccgaattt tggtgcgcag gtggtggctg cagtgctgaa tgacgaggca ttgaaagcca
     9781 gctggctggc ggaagtagaa gagatgcgta ctcgcattct ggcaatgcgt caggaattgg
     9841 tgaaggtatt aagcacagag atgccagaac gcaatttcga ttatctgctt aatcagcgcg
     9901 gcatgttcag ttataccggt ttaagtgccg ctcaggttga ccgactacgt gaagaatttg
     9961 gtgtctatct catcgccagc ggtcgcatgt gtgtcgccgg gttaaatacg gcaaatgtac
    10021 aacgtgtggc aaaggcgttt gctgcggtga tgtaatgcag gaaagcaggc tggagctacc
    10081 cagcctgcag tgaaattaaa ctgtcgtcgc tttcactctt tctttataga tgattttttt
    10141 gatgccatcg ttctacgtga gagataataa acgttattag ttcttttatt gttaagctta
    10201 tcctaattat ctggaattcc ttatcctgtt tttttgggtg gagtaataat ttctgcacat
    10261 ctaactatta ttaaatttaa taataagtat cgggtttgtt acgatagaat ttttctatat
    10321 cctcaagaat atgctttgcc ttattgcctg tgactttctg agattcaatt atagtatcgt
    10381 taaattctaa agttaaagag agctcttttt ttccgctttg tgtaagttta accttatatt
    10441 gattaccaat atcaaataaa aaggtcctgt aatgagaata atcaccttta acctcctcgg
    10501 caaaacagca aagcgtgcaa atatacataa agtctttctt acccctgatt ttgttaacta
    10561 aatcaataaa tgcgtgaaaa actttacttg caatagaact tgatacttct tctccaggtc
    10621 tacaaaaatc ttttgcaatt tttaactgtg tttctatgta taaaaagaaa tctttggcat
    10681 aagaaaaatc tttggcataa gagatatctt tattatttag tgcatctagc tcaacaggta
    10741 aagatgtcgt tttgttgcta ttcacatatg atatattcat atcatcatct ccatcgcgag
    10801 taataaaatt aatcaccatt gtagggtagg gggctggtca atcagaaatc atctttataa
    10861 acttcgattg tttttgtaat gctgtatcat taagttcatt aaatcgtaca gcagataaat
    10921 gttctatcaa atttcgctca tttgccgagg attcatcata ataaacgtaa aattaatgta
    10981 tccttacatc gagtaataaa cattttttat acaaaaaaag acaggaacgt atttactggg
    11041 ttaaatataa tcatcctgct tttcatcaca aaaaccgcag ataatccttc ctttccccgg
    11101 cagctggcgt tatggtcaga tggtttttgc aacaaatctc acaataaaaa gtttcaacat
    11161 actgactatt tagggaaaaa tatgcgcaag atcacacagg caatcagtgc cgtttgctta
    11221 ttgtttgctc taaacagttc cgctgttgcc ctggcctcat ctccttcacc gcttaaccct
    11281 gggactaacg ttgccaggct tgctgaacag gcacccattc attgggtttc ggtcgcacaa
    11341 attgaaaata gcctcgcagg gcgtccgcca atggcggtgg ggtttgatat cgatgacacg
    11401 gtactttttt ccagtccggg cttctggcgc ggcaaaaaaa ccttctcgcc agaaagcgaa
    11461 gattatctga aaaatcctgt gttctgggaa aaaatgaaca atggctggga tgaattcagc
    11521 attccaaaag aggtcgctcg ccagctgatt gatatgcatg tacgccgcgg tgacgcgatc
    11581 ttctttgtga ctggtcgtag tccgacgaaa acagaaacgg tttcaaaaac gctggcggat
    11641 aattttcata ttcctgccac caacatgaat ccggtgatct ttgcgggcga taaaccaggg
    11701 caaaatacaa aatcgcaatg gctgcaggat aaaaatatcc gaatttttta tggcgattct
    11761 gataatgata ttaccgccgc acgcgatgtc ggcgctcgtg gtatccgcat tctgcgcgcc
    11821 tccaactcta cctacaaacc cttgccacaa gcgggtgcgt ttggtgaaga ggtgatcgtc
    11881 aattcagaat actgacagag cgggagagcg tgacgctctc ccgaatgctg tttttttaat
    11941 cacaccttta tcctttcgct gtcttgctgc aaactgatta agagagtttt atcaaggagc
    12001 agcacatgtg gtatcaaaag acgctcacgc ttagcgccaa atctcgtggg tttcatctgg
    12061 taacggatga aattctgaat cagctggctg atatgccgcg cgttaacatc ggcttactgc
    12121 atctgttgct gcaacatacc tccgcctctc tgacacttaa tgagaactgc gatcccaccg
    12181 tacgccacga catggagcgt tttttcctcc gcaccgttcc cgacaacgga aattatgagc
    12241 atgaatatga gggagcagac gatatgcctt ctcatatcaa atcctcaatg ctgggaacat
    12301 cgcttgtatt gccggtacat aaagggcgta ttcagaccgg cacctggcaa ggcatctggc
    12361 tgggggaaca tcgcatccac ggcggatcgc gtcgcatcat cgcgacacta caaggggagt
    12421 aaaaaatgac catttcggag ttgctacaat attgcatggc aaaaccaggc gcagaacaga
    12481 gcgtgcataa cgactggaaa gcgacgcaga tcaaagtgga agatgtactg tttgcgatgg
    12541 tgaaagaagt agaaaatcgc ccagctgttt cgctgaaaac cagcccggag ctggcggagc
    12601 tgctacgtca gcagcacagc gatgtgcgtc ctagccgcca tctgaataaa gcgcactgga
    12661 gcaccgtgta tctcgacggt tcgctgccgg attcgcaaat ctattatctg gtggatgcgt
    12721 cttatcagca ggcagtgaat ttactaccgg aagaaaagcg taaattgctg gtacaactct
    12781 gaaaggaaaa ggccgctcag aaagcggcct taacgattac agcatcggct tgaggaagcg
    12841 tgccgtgtgt gatgcttcgc actccgcgac ggtttctggc gtaccggaga cgaggatttc
    12901 gccgccgcca ctgccgcctt ctggtcccag gtcgacaatc cagtcagcgg ttttgatcac
    12961 gtcgagattg tgctcaatca ccacaatggt gttgccctga tcgcgcagtt tatgcagtac
    13021 gtcgagcagt tgctgaatat cggcgaagtg cagaccggtg gtcggctcgt cgagaatata
    13081 cagcgtctgc ccggtgccgc gttttgacag ctcacgcgcc agcttcacgc gctgggcttc
    13141 accaccagaa agtgtggttg cggactgccc caggcgaatg tacgtcaggc caacgtccat
    13201 caacgtttgc agcttacgcg ccagtgcagg tacggcatca aagaactcac gcgcctcttc
    13261 gatggtcata tccagcactt cgtggatggt tttgcctttg tacttaattt ccagcgtttc
    13321 acggttatag cgtttacctt tacactgatc gcacggcacg tagatatccg gcaggaagtg
    13381 catctctact ttgatcacac cgtcgccctg acaggcttcg cagcgcccgc cacggacgtt
    13441 aaagctgaaa cgtcctggcg tataaccacg cgcacgggat tccggtacgc ccgcaaacag
    13501 ttcgcgcaca ggcgtaaaca cgccggtata ggtcgccggg ttagaacgtg gagtacgacc
    13561 aattgggctt tggtcgatat cgatcacttt atcgaaatgc tccagcccct gaatatcgcg
    13621 atacggtgcc ggttcggcga tggtcgcacc attcaactgg cgttgggcaa tcgggaacag
    13681 tgtgtcgtta atcagcgtcg atttaccgga acctgaaacc cctgtgatgc aggtaaacag
    13741 accgactggc agcgtgagcg tcacgtcctt caggttgttg ccgcgtgcgc ctgtcagctt
    13801 cagcactttt tccggatttg ccggaacgcg tttcttcggc acttcaatct tgcgtttacc
    13861 gctcatgtac tgtccggtta acgactccgg caccgccata atcgcttcca gtggaccttc
    13921 cgcgaccact tcaccgccgt gaacacccgc accaggaccg atatcgatca catggtcagc
    13981 ggcgcgaatt gcgtcttcgt catgctccac cacaatcacg gtattaccga gatcgcgcag
    14041 atggataagc gtacccaaca ggcgctcgtt atcgcgctgg tgcaggccga tagacggctc
    14101 gtccagtaca tacataacgc caaccaggcc cgcaccaatc tggctcgcca gacggatacg
    14161 ctgggcttca ccgccggaaa gtgtttctgc cgagcgggaa agtgtcaggt aattcaggcc
    14221 gacgttaacg aggaatttca gacgatcgcc gatctcttta aggatttttt ctgcaatctt
    14281 cgcccgctga ccagctagtt tgagattgtt gaagaattcc atcgcatgac cgatgctcat
    14341 gtcggagata gcaggcagcg gcgtattctc gacataaacg tggcgcgctt cccgacgcag
    14401 acgcgtccct tcgcagctgg cgcatgggcg attgctgata aacttggcta attcttcacg
    14461 taccgcactg gattctgtct ctttataacg gcgctccata ttgtgcagca cgccttcgaa
    14521 cggatgacga cggatggagg tatcgccacg atcgttcatg tatttgaatt caatgttttc
    14581 tttgccagaa ccgtacaaca ccactttatg cacgttcgcg ctcaggctgc cccacggcgc
    14641 ttcgacgtcg aacttatagt gatctgccag cgatttcagc atctggaagt aatagaagtt
    14701 gcggcgatcc cagccacgga tcgcaccgcc agccagtgac agctcggggt tttggatcac
    14761 gcggtcagga tcgaaatatt gctgtacgcc aaggccgtca caggttgggc aggcacctgc
    14821 cgggttgtta aacgaaaaca gtcgcggctc cagttcacgc atactgtagc cgcaaattgg
    14881 gcaggcgaag ttggcggaga acagcagctc ttccgctttc gggtcgtcca tatccgccac
    14941 taccgctgta ccaccggaaa gctccagcgc ggtttcaaac gactccgcaa gacgttgggt
    15001 aagatcgtca cgcaccttga agcgatcaac caccacttca atagtgtgtt tcttttgcag
    15061 ttccagtttc ggcggatcgg aaagatcgca gacttcgcca tcaatacgag cacggatgta
    15121 accctggctt gccaggttct ccagcgtttt ggtgtgttcg cctttgcgct ctttaatgat
    15181 tggcgcgagt agcatcagac gcttgccttc cggctgcgac agcacgttat ccaccatctg
    15241 gctgacggtt tgcgccgcca gcgggacgtc gtggtccgga cagcgcggtt cgccgacgcg
    15301 ggcgaacaac aaacgcaaat agtcgtggat ttcggtgatt gtccccaccg tagaacgcgg
    15361 gttatgagac gtcgatttct gctcaattga gatggcagga gaaagcccct caatatgatc
    15421 gacgtccggc ttttccatca gtgacagaaa ctgccgcgcg taggcggaaa gggattcaac
    15481 gtaacggcgc tgcccttcgg catataaggt gtcgaaagcg agcgaggatt tgccagaacc
    15541 cgaaagcccg gtcacgacaa tgagcttgtc gcgggggata acgaggttga tgtttttgag
    15601 attatgggtg cgggcgcccc gaacttcgat cttatccatt cacctttccc ggattaaacg
    15661 cttttttgcc cggtggcatg gtgctaccgg cgatcacaaa cggttaatta tgacacaaat
    15721 tgacctgaat gaatatacag tattggaatg cattatccgg agtgttgtgt aacaatgtct
    15781 ggccaggttt gtttcccgga accgaggtca caacatagta aaagcgctat tggtaatggt
    15841 acaatcgcgc gtttacactt attcagaacg atttttttca ggagacacga acatggccag
    15901 cagaggcgta aacaaggtta ttctcgttgg taatctgggt caggacccgg aagtacgcta
    15961 catgccaaat ggtggcgcag ttgccaacat tacgctggct acttccgaat cctggcgtga
    16021 taaagcgacc ggcgagatga aagaacagac tgaatggcac cgcgttgtgc tgttcggcaa
    16081 actggcagaa gtggccagcg aatatctgcg taaaggttct caggtttata tcgaaggtca
    16141 gctgcgtacc cgcaaatgga ccgatcaatc cggccaggat cgctacacca cagaagtcgt
    16201 ggtgaacgtt ggcggcacca tgcagatgct gggtggtcgt cagggtggtg gcgctccggc
    16261 aggtggtaat atcggtggtg gtcagccgca gggcggttgg ggtcagcctc agcagccgca
    16321 gggcggttgg ggtcagcctc agcagccgca gggtggcaat cagttcagcg gcggcgcgca
    16381 gtctcgcccg cagcagtccg ctccggcagc gccgtctaac gagccgccga tggactttga
    16441 tgatgacatt ccgttctgat ttgtcattaa aacaataggt tatattgttt taaggtggat
    16501 gattaaagca tctgccagcc ataaaaaaga agcctccgtt atggaggctt ctacgtatca
    16561 ggtcaaaatc attggccatt gtggcggtgt ctgattcatc tcttctatca tcaccgactc
    16621 gatgttgtgc cagatagcgg tgatgtccgc cattgtgatg ccaagcaacc ccattgcgaa
    16681 ccaacaggcg gaaacaacgc ccagaccgct gctgatcacc gcaagcccga cataatcaga
    16741 ccgacgaaaa cggatgttga gtgtgctggc cagaaacatc attacggcac taagaagttg
    16801 ccagcgaaga agaaccacgc cagtggtgag ggtagccatc aaacaattcc tctgaaaaga
    16861 gccgatgccc tggacagcgc ggcgttgttc acgggaggcg tggtacactc tggctatcgc
    16921 ggggcttgca gaacacaaaa atgaaacact ctgtttgttt cattaatttt gtgaactata
    16981 tcacaattga ttgtttgtta gccatattag gccgtgactt ttattgctgt acagattatg
    17041 tggtttttca gtggcattaa gggcatatct tcccgccgcc tctgcattcc tgtaggaaat
    17101 taattttgaa tatcaatgaa ttattttcat ccaggtgacg attagaaagg tatcaatttc
    17161 aaatcaggca aaagtgctat ttataccgta agatttatct aaagacgtcg gtacccaggg
    17221 ttttcacctt gcaatggccg ggtataaaca ggcaggaaat tgatagcaat gagtcatcgt
    17281 gcacgacacc aattactggc gttgccgggc attatctttt tagttctctt tcccatcatt
    17341 ctttcgctat ggattgcctt cctttgggca aaatcagaag tgaataatca gctccgaacc
    17401 tttgctcaac tggcactgga taaatccgag ctggtcattc gccaggcaga tttagtgagc
    17461 gatgcagctg aacgctatca ggggcaagtt tgcactccag cccatcaaaa gcgaatgttg
    17521 aatattattc gtggctatct ttatattaat gaattgatct atgcccgtga taaccatttt
    17581 ttatgctcat cgctgatagc gcctgtaaac ggctatacga ttgcaccggc cgattataag
    17641 cgtgaaccta acgtttctat ctattattac cgcgatacgc cttttttctc tggctataaa
    17701 atgacctata tgcagcgggg aaattatgtg gcggttatca accctctctt ctggagtgaa
    17761 gtgatgtctg atgacccgac attgcaatgg ggtgtgtatg atacggtgac gaaaaccttt
    17821 ttctcgttaa gcaaagaggc ctcggcagca acgttttctc cgctgattca tttgaaggat
    17881 ttaaccgtac aaagaaatgg ctatttatat gcgacagttt attcgacaaa acgcccaatt
    17941 gcagccattg ttgcgacttc atatcaacgt cttataaccc atttttataa tcatcttatt
    18001 tttgcgttgc ccgccggtat tttggggagt cttgttctgc tattactctg gctacgtatt
    18061 cgacaaaact atttatctcc caaacgtaaa ttgcaacgcg ccctcgaaaa acatcaactt
    18121 tgtctttatt accagccaat aatcgatatc aaaacagaaa aatgtatcgg cgctgaagcg
    18181 ttgttacgtt ggcttggtga gcaggggcaa ataatgaatc cggcagagtt tattccgctg
    18241 gcagaaaagg aggggatgat agaacagata actgattatg ttattgataa tgtcttccgc
    18301 gatctgggcg cgtacttggc aacacatgcc gatcgctatg tttctattaa cctgtcggcc
    18361 tccgattttc atacgtcacg gttgatagcg cgaatcaatc agaaaacaga gcaatacgcg
    18421 gtgcgtccgc agcaaattaa atttgaagtg actgagcatg catttcttga tgtcgaaaaa
    18481 atgacgccga ttattctggc tttccgccag gctggttacg aagtggcaat tgatgatttt
    18541 ggtattggct actctaactt gcataacctt aaatcattga atgtcgatat tttgaaaatc
    18601 gataaatcgt ttgttgaaac gctgaccacc cacaaaacca gtcatttgat tgcggaacac
    18661 atcatcgacc tggcgcacag cctggggtta aaaacgatcg ctgaaggcgt cgaaactgag
    18721 gagcaggtta actggctgcg caaacgcggc gtgcgctatt gccagggatg gttttttgcg
    18781 aaggcgatgc cgccgcaggt gtttatgcaa tggatggagc aattacccgc gcgggagtta
    18841 acgcgcgggc aataaaacta caggcggtgg cgataatcgc tgggagtgcg atcaaactgc
    18901 cgacggaaaa cgcgggagaa ggtctgctgc gagacataac ccaggtccat tgcgatatca
    18961 aaaatcggac gctcggtggt gcgcaactca acggcggcca gtaacaggcg acgttggcga
    19021 atgtaatcgc caagcgtctg atgcgtcacc gtgcggaaca ttcgttgcaa gtaccacttt
    19081 gaatagcctg atttttttgc gactacatca atgttaagcg gctggtcaat atgctcgtca
    19141 atccatgcga taagatcctg aataattttc tgatgggaca taaatctgcc tcttttcagt
    19201 gttcagttcg ttaattcatc tgttggggag tataattcct caagttaact tgaggtaaag
    19261 cgatttatgg aaaagaaatt accccgcatt aaagcgctgc taacccccgg cgaagtggcg
    19321 aaacgcagcg gtgtggcggt atcggcgctg catttctatg aaagtaaagg gttgattacc
    19381 agtatccgta acagcggcaa tcagcggcga tataaacgtg atgtgttgcg atatgttgca
    19441 attatcaaaa ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc
    19501 gtgttgcccg aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg
    19561 cgagaagagt tggatcggcg cattcatacc ttagtagcgc tgcgtgacga actggacgga
    19621 tgtattggtt gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc
    19681 ttaggagaag aaggtaccgg cgcacgcttg ctggaagatg aacaaaacta aagcgccaca
    19741 agggcgcttt agtttgtttt ccggtctttg tctttctctc tatcccgctg gtacacagga
    19801 gggtttcccc cgacgtcaac acaccccatt cgagcacgtg gtggaggttc cggttggtgt
    19861 tgatgcttta attgtatgtc accggcattt cttcgccagt gtaaaagtat actttttatc
    19921 cgcaatattt ttgtcatctc agacgatttt ttatcgcaat cctgaacggt atacggctcg
    19981 ataacgctgc aatcttgcgc accgacgata acgtttgcgc atcaattgcc tggtttttca
    20041 tcgtcaagac aataaaagag aaaaaagcag caaacttcgg ttgaaaaagc cgctatgatc
    20101 gccggataat cgtttgcttt ttttaccacc cgttttgtat gcgcggagct aaacgtttgc
    20161 ttttttgcga cgcagtaaat tgtcgcaaac ctggagcagg aagataacgt ttcgctggca
    20221 ggggattgtc cgccacgcat cttgacgaaa attaaactct caggggatgt tttcttatgt
    20281 ctacgccatc agcgcgtacc ggcggttcac tcgacgcctg gtttaaaatt tcacaacgtg
    20341 gaagcactgt ccgtcaggaa gtggttgccg ggttaacaac gtttctggcg atggtctact
    20401 cggtcatcgt cgttccaggt atgttgggta aagcaggctt cccgcctgcg gcagttttcg
    20461 ttgcaacctg tctggttgcc ggactcggtt ctatcgtgat gggtctgtgg gctaatctgc
    20521 cgttggcgat tggttgcgcc atctccctga cggcgtttac cgcattcagc ctggtgctgg
    20581 ggcaacatat tagcgtacct gtcgcgctgg gtgccgtgtt cctgatgggt gtgctgttta
    20641 cggtaatttc tgccacgggt atccgtagct ggattttgcg caacttgccg cacggtgtgg
    20701 cgcacggcac ggggattggt atcggtctgt tcctgctgct cattgccgct aacggtgtcg
    20761 gtctggtgat taaaaacccg cttgatggtc tgcccgttgc gctgggtgat ttcgcgacct
    20821 tcccggtgat tatgtcactg gtaggtctgg cggtgatcat cggcctggaa aaactgaaag
    20881 tccctggtgg cattctgctg accattatcg gtatctcaat tgtcggtttg atcttcgatc
    20941 ctaacgtcca tttctccggc gttttcgcca tgccttcatt gagcgatgaa aacggcaact
    21001 cactgattgg cagcctggac attatgggcg cgctgaatcc tgtagtcctg ccaagcgttc
    21061 tggcgctggt aatgacggca gtatttgatg ccaccggaac tatccgtgcc gtcgccggcc
    21121 aggcgaacct gctggataaa gatgggcaaa ttatcgacgg tgggaaagca ctgaccactg
    21181 actccatgag cagcgttttc tctggcctgg tgggtgcagc tccggcagcg gtatacatcg
    21241 agtctgcggc gggtacggcg gcgggcggta aaaccggttt gacggctatc accgttggcg
    21301 tgctgttcct gctgattctg ttcctctctc cgctctctta cctcgttccg gggtatgcaa
    21361 cggctccggc gctgatgtac gttggcctgc tgatgctgag caacgtggcg aaaatcgact
    21421 ttgctgattt tgttgatgcg atggcgggtc tggttacggc ggtattcatc gtgctgacct
    21481 gtaacatcgt aacaggcatc atgatcggct tcgcgactct ggtgattggt cgtctggttt
    21541 ccggcgaatg gcgcaagttg aacatcggta cggtcgttat cgccgtggcg ctggtgacct
    21601 tctatgcggg tggctgggct atctaatctt tcatcggatt ctgaaaacgg gtggcaatgg
    21661 ctgcccgttt ttattttctc cgcacattgt gtgagctttt gcgatattct gaaaaaatga
    21721 gaattcaggc ataacgtctt tctcagatca taaaaaacat cgcaaacagg gaacgcatgg
    21781 aaatcttctt caccatactg ataatgaccc tcgtggtctc gctgtccggg gtggtcactc
    21841 gtgtcatgcc ctttcagatc ccgcttccgc ttatgcaaat cgccatcggt gcgctactgg
    21901 cgtggccgac gtttggtttg catgtggagt ttgatcctga actcttttta gtcttgttta
    21961 tcccgccgtt gctgttcgct gatggctgga aaacgccgac ccgtgaattt ctcgaacatg
    22021 gtcgagagat tttcggcctc gcgctggcgc tggtggtggt caccgtggtc ggcattggct
    22081 tccttattta ctgggtggtg ccgggcattc cgctgatccc cgcctttgcg ctggcggcgg
    22141 tactttctcc gaccgatgct gtggcgctct ccgggattgt tggcgaaggg cgcatcccga
    22201 aaaaaatcat gggcattttg cagggcgaag cgttgatgaa tgacgcttcc ggcctggtgt
    22261 cgttgaagtt tgccgtggca gtggcgatgg ggacgatgat cttcaccgtc ggcggtgcga
    22321 cggtcgaatt tatgaaagta gccattggcg gtattcttgc cggttttgtg gtgagctggc
    22381 tgtatggtcg ctcgctgcga ttcctcagcc gctggggcgg tgatgaaccc gcgacgcaga
    22441 ttgttctgct gttcttgctg ccattcgctt cttatctgat tgccgaacat attggcgttt
    22501 ccggcatcct cgctgcggtt gccgccggga tgaccatcac ccgctccggt gtgatgcgcc
    22561 gtgcgccgct ggcaatgcgc ctgcgtgcaa acagcacctg ggcgatgctg gaatttgtct
    22621 ttaacggcat ggtgttcctg ctgttaggtc tgcagctgcc gggtattctg gagacgtcgc
    22681 tgatggcagc agaaatcgac cctaacgtcg aaatctggat gctgtttacc gatattattc
    22741 tgatatatgc ggcgctgatg ctggtccgtt tcggctggct gtggacgatg aaaaagttca
    22801 gcaaccgctt cctgaagaag aagccgatgg agtttggttc gtggaccaca cgagaaatcc
    22861 tgatcgcgtc tttcgccggg gtgcgtgggg cgatcactct ggccggtgtg ctctcaatcc
    22921 cgctgctctt gccggatggt aacgtcttcc cggcgcgcta tgagctggtg ttcctggcgg
    22981 ctggcgtcat tctcttctcg ctgtttgtcg gcgtggtgat gttgcctatt ctgctacaac
    23041 acattgaagt cgcggaccat tcgcaacaat tgaaagaaga acgtattgcg cgagcggcaa
    23101 cggcagaagt ggcgattgtg gcgatccaga aaatggagga gcgtctggcg gcggataccg
    23161 aagagaatat cgataaccag ctgcttacgg aggtcagttc tcgcgtcatt ggtaacctgc
    23221 gtcgtcgcgc cgatggacgt aacgacgttg aaagttccgt gcaggaagag aaccttgagc
    23281 gtcgcttccg tctggcggca ttgcgttctg aacgtgctga actgtaccac ctgcgcgcca
    23341 cgcgggagat cagcaacgaa acgctgcaaa aattactgca cgatctcgat ttgcttgaag
    23401 cgttgctaat tgaggaaaat cagtaagccg aaagcgacgt aaccaggccc gaggtaatct
    23461 gaaaaaggtg caatcttttg cacctttaac aggttagctc gggctaacat tcatgattct
    23521 aaaacaaata agttatcctc ttgtaaagtc aattcattgg ctgttcttcg acttaacctc
    23581 aatctgcgtg ttgcataata tctgcagtaa tcttcatagc ggctgcatta atattatcct
    23641 cagtttgtga catgccagta tgagaggtac aatcttcttc aaagaatttc tgtagtgtta
    23701 ccagggtatc actatcaatg gggatatttt cctccccacg gagaatggaa atgcccattc
    23761 cttggacgct agcctcatcg aaattaaccc gatagagacg ggtattaaac atacaggctt
    23821 ttgttaaaat agaacagttg aaatggcagt tgcttaaatt ggcatcatta aaatcaacct
    23881 tagccatatt agagccggta catataacca tttcgaggaa tgtatttgtc agccacgtac
    23941 aaaagagttt gctatcaatt aagtttatat tctggaggat tgctttgttc agattgacgc
    24001 gagacagatc agcatgttca aagttaatta aatcgagttt agagaaacta aggtcggctt
    24061 taaataaatt aacatatggc gctattactt tataaagatt tacctcagcc atgtacgcca
    24121 tataaaaatt tgaatagtca aggaaggtat ttttaatatt ggctctattc attgctgcat
    24181 tgtagaattg aacatgcata gcggagcatg catttagagt cccgtcagtc atttttacat
    24241 gagcgaaata tgagtcatcg agaatacaat tatcaaaaag tattttttcc attatggcgc
    24301 aataaaaatt cgttctatac atattacagt cctggaaaat agagttttgt agtctgcaac
    24361 ctgcaaaatt cgaattggat aaatcttcat ctttaatcac gatacctttc aggctaaggt
    24421 gtgaaaaatc gtaaccacca ttttctgcct gtgggatatc gatagtatta ttatggacgg
    24481 tgacattatt tataaaatat accaccttaa ttgacacctc cttatcttta ttaaggtaaa
    24541 ctcttctcgt aattctatcg tcacatttat tatttgcata tgaagctata tctataaaat
    24601 atttatgtat tttcctgtcc gtttctttcg tgatttttcc tgatggtaaa ctatcataaa
    24661 ttcttttcat tttctgaatc aagcctatca ctttttcatg atcatccatt ttgaaaagag
    24721 agagggcttt attgtcaatt aacgtgtttt tatgatgttt gctgtaatta tctgattttg
    24781 ttttctttgt atgtgatgaa cttgtgacag agtggttatc attaatctgg caaagaggaa
    24841 aaaacatatt atttaaacca ttatagcgca taaaatatcc ttataattaa caagaaacaa
    24901 aaggcatatt ctctgcatta tcattttcta cgactgtcaa aaatcgctca ttttttaatg
    24961 agtttatttg tttaatatta tgggaaaagg tgatgcatgt tggagaggaa gagtattccc
    25021 cggtcaggcg accggggaag gggagagatt aatgtgcgcg gccttgctca acgccaaagc
    25081 cggtctggga gcggataaac tgtgcacgga acagttcacg ctcgcgcgca ccttccgctg
    25141 agttatcggt tgccgagaag aaccagatgc cgaggaatgc cacgctgata gagaacagcg
    25201 ccgggtattc atacgggaag atggcttttt cgtgaccaag gatctgtacc caaatcgtcg
    25261 ggccgaggat catcagtacc actgcggtaa tcagccccag ccagccaccc atcatcgcgc
    25321 cacgagtggt cagtttcgac cagtacatag aaagcagaat gatcgggaag ttacagctcg
    25381 ctgcgatggc aaacgccagg cctaccatga aggcgatgtt ctgattctca aacagcacgc
    25441 cgaggataat ggcaatcaca cctaaaatca gcacggtgat tttcgatacc cgcagttctt
    25501 cacgttcggt cgcgcctttt ttgaagacgt tagcgtacag gtcatgcgaa accgctgatg
    25561 cgcccgccag cgtcagacca gcaaccaccg caagaatggt ggcaaaagca accgctgaaa
    25621 taaaaccgag gaacaggttg ccgcccactg cattcgccag gtgaacggcc gccatgttgt
    25681 taccaccaat cagatggccc gccgcgtctt tatattccgg attcgcacca accagcatga
    25741 tcgcgccgaa gccgataata aaggtcagaa tatagaagta gcccataaac ccggtggcgt
    25801 agaacacgct cttacgtgct tcgcgggcat cgctgactgt aaagaagcgc atcagaatgt
    25861 gcggcaagcc cgccgtacca aacatcagtc ccagacccag agagagcgcg gagatcggat
    25921 ctttcaccag cccgcccggc ttcatgatgt cgacaccttt cgggtgtacc gccatcgctt
    25981 cactgaacag attgttgaag ctaaagccga cgtgtttcat caccataaag gccataaagc
    26041 tggcaccgaa cagcaacagc acggctttga taatctgtac ccaagtggtc gccagcatgc
    26101 cgccgaacag gacgtacatc atcatcagca cgccgaccag caccaccgca atgtgatagt
    26161 taaggccaaa cagcagctcg atcagtttac ctgcgcccac catctgggcg ataaggtaaa
    26221 gcgccaccac caccagagaa ccacaggccg aaagaatacg aatcggccct tgtttcagac
    26281 ggtaagaggc cacatcggca aaggtgtagc gccccaggtt acgcagacgt tcggcaatca
    26341 ggaacaaaat gatcggccag cccaccagga agcccagcga gtaaattaag ccgtcatagc
    26401 cggaggtaaa caccagcgcg gagatcccca agaatgaggc ggcggacata tagtccccgg
    26461 caatcgccag cccgttctgg aagccagtga tattgccgcc tgcggtgtag tagtcgctac
    26521 gagaacgtac gcgttttgat gcccagtagg taatgccgag cgtaaacacg acgaaaatca
    26581 ggaacataat aatcgcctgc cagttcgttg gctggcgctc tacggccccg ctaatagcat
    26641 ccgcggcgtt agctgcgaaa gggagtgtgg cggcaagcgc cgtcagaact cttttcatga
    26701 tgcttgtacc tcatgcagga cttcattatt aagacggtcg aattcgccgt tcgcccgcca
    26761 gatgtagata ccggtgagaa caaaggagat cacaatcact ccaacaccaa ttggaatacc
    26821 gcgtgtgacg ctggtgttcg gattcagcgg cgtgcccagc cagccgggcg cgaaggcgat
    26881 cagtaaaata aagccgatat aaactgccag cataataatc gacaggatgg tggcaaaccg
    26941 ttgccgtttt tcgactaact ccctgaaatg cgcattgtct tctatccgct gataaatagt
    27001 gccattcatc acagattctc cagaggtaat gtagggattg ttttaattcc cgctccctta
    27061 tgggagaagg ttaacgctcg ggtaaccctt gccgaatgta ggccggataa ggcgtttacg
    27121 ccgcatccgg caatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg
    27181 ttaccgactc gcatccggca attgtgggtt acgatggcat cgcgatagcc tgcttctctt
    27241 caagcagctt ctcgactacg ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg
    27301 tatcgcccgc cgcaattttg cgcagaatac ggcgcataat tttgccggag cgggttttag
    27361 gcagggagtc ggtccagtgc agcacgtctg gcgtcgccag cgggccaatc tctttacgca
    27421 cccagttgcg gacttctgcg tacagttctg gtgacggttc ctccccgtga ttaagcgtga
    27481 cgtaggcgta gatcgcctga cctttaatat tgtgcggaat acctactacg gcggcttcgg
    27541 caatcttcgg atgcgccacc agcgccgact caatctctgc cgtccccaga cggtgaccgg
    27601 agacgttcag cacgtcgtcc acacgcccgg tgatccagta atagccatct tcatcgcgac
    27661 gcgcgccgtc gccgctgaaa tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac
    27721 gttcgtgatc gccaaacagc gtacgcgcct gacccggcca ggagtcggtg atcaccaggc
    27781 tgccttcggt agcgccttcc agcgggttac cttcgttatc gaccagcgcc ggttgcacgc
    27841 cgaagaacgg acgtgttgcc gaaccggctt tcagctcggt agcgccaggc agcggcgtga
    27901 tcatgaaacc gccagtttcg gtctgccacc aggtatcgac caccggacat ttctcgttgc
    27961 cgattttttt ccagtaccac tcccacgctt ccgggttaat tggctcgccc acggaaccga
    28021 gaatgcgcag cgacgaacgg tcggtgcctt cgatcgcttt atcgccttcc gccatcagcg
    28081 cgcggatcgc cgtgggtgcg gtatagagaa tattgacctg atgcttgtcc accacctgcg
    28141 ccatacgggc aggcgtcggc cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc
    28201 aggccagcgg gccgtacagc aagtaactgt gtccggtcac ccagcccaca tcggcggtgc
    28261 accagtagat atcacccgga tgataatcaa agacatattt aaaggtcagc gccgcgtaca
    28321 ccagataacc gccggtagta tgcagcacac cttttggctt accggtagaa ccggaggtgt
    28381 agagaataaa cagcggatct tcggcgttca tctcttccgc ctggtgctga tcgctcgctt
    28441 gctcaaccag gtcgtgccac cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag
    28501 tacgcttcag taccaccaca tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat
    28561 caacgttttt cttcagcgga atactgcgcc cggcacgcac accttcgtcg gaagtgatca
    28621 ccagtcgtga gttggaatca ataatgcgcc cggcaacggc ttccggcgag aagccgccga
    28681 aaatcaccga atgcaccgcg ccaatgcggg cgcaggccag catcgcaacc gcggcttccg
    28741 gcaccatcgg catataaatc gccaccacat cacctttttt aatgcccagc tcgagcaggg
    28801 tattggcgaa gcggcagacg tcgcggtgca gctctttata gctgatatgt ttgctctggc
    28861 tggcgtcgtc gccttcccag atgatggcgg tgcgatcgcc gttttcttgc agatggcggt
    28921 caaggcagtt tgccgccaga ttcagcgtgc cgtcctcgta ccatttaatg gacacattac
    28981 cgggggcaaa ggaggtattt ttcaccttct ggtacggttt gatccagtcg agaatttttc
    29041 cctgttcgcc ccagaaggta tcaggtacgt taatagattg ttgatacatc gcctcgtact
    29101 gctgagggtt tatcaggcaa cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt
    29161 ggctcatgct tttgttctcc ttgtgggatg ttaataatat gtggcataag cgttaaatgt
    29221 aggggtattg gcagttttgt ttagtatttg ggcgacagat cacgcaaaag tagaattgtg
    29281 caaataaacg gcagggtaat ttttgaaggt caccaacaaa agttgattaa ttctttgagg
    29341 aacatgcagt tatgcatgct gttgaaaaag aggaagatac tgactaactc taaagtggta
    29401 ttttacatgc acttacaatt gattaaagac aacattcaca gtgtggttat ttgttacaca
    29461 taggggcgag caatgtcatg acagtgtagg tgcggttact gtcgtgaaaa caataaaaac
    29521 cgccattgca acaatggcgc aattcggatg aagcccctat gacaaggata aaaataaacg
    29581 cacgccgtat cttcagctta ttgattcctt ttttcttttt cacttctgtt cacgctgaac
    29641 aaacggcagc tcccgcaaaa cctgtaactg tggaagcgaa gaatgaaacc tttgccccgc
    29701 agcatcccga tcaatatctc tcctggaaag ccacctcgga acagtcagag cgtgttgacg
    29761 ccctggcgga agatccacgg ctggtgatcc tgtgggcggg gtatcccttc tcgcgcgatt
    29821 acaacaagcc gcgtggacat gcctttgctg tgaccgatgt gcgtgaaacc ctgcgtaccg
    29881 gtgcgccgaa aaacgctgaa gatggtccgc taccgatggc gtgctggagt tgtaaaagcc
    29941 cggatgtggc gcgtctgatc cagaaagacg gcgaagatgg ctacttccac ggtaagtggg
    30001 cgcgcggcgg cccggaaatc gtcaacaact taggttgtgc cgactgccat aacaccgcct
    30061 caccagagtt cgccaaaggc aaaccggagt taaccctttc ccgtccgtat gcggctcgcg
    30121 cgatggaagc cattggtaaa ccttttgaga aagccggacg tttcgaccag caatcgatgg
    30181 tttgcggtca gtgccatgtg gagtattact tcgacggcaa aaacaaagcg gttaaattcc
    30241 cgtgggatga cggcatgaaa gtcgaaaata tggagcagta ttacgacaaa attgccttct
    30301 ctgactggac taactccctg tcgaaaacgc caatgctgaa agcgcagcac ccggaatatg
    30361 aaacctggac agcgggcatt cacggtaaaa acaacgtgac ctgtatcgac tgccatatgc
    30421 caaaagtgca gaacgccgaa ggcaaactct acaccgacca taaaattggt aatccgtttg
    30481 ataacttcgc ccagacttgt gcgaactgcc atacccagga caaagctgcc ttgcaaaaag
    30541 tggtcgcgga acgtaagcag tcgattaacg acctgaaaat caaggttgaa gatcaactgg
    30601 ttcacgctca cttcgaagcg aaagcagcgc tggatgcagg cgcgacggaa gccgaaatga
    30661 agccgattca ggacgatatc cgtcatgccc agtggcgttg ggatctggcg atcgcttccc
    30721 acggcattca tatgcacgca ccggaagaag gtttacggat gctcggtact gcgatggata
    30781 aagcggcgga tgcacgcacc aaactggcgc gcctgctggc gaccaaaggc atcacccatg
    30841 aaatcgagat cccggatatc tcgaccaaag agaaagccca gcaggccatt ggcctgaaca
    30901 tggaacaaat caaggccgag aagcaggact tcatcaaaac ggtgatcccg cagtgggaag
    30961 aacaggcacg taaaaatggt ctgttaagcc aataaccccg ttccgactcg caaggggcgg
    31021 aaaacacaat ggagtgaata tgagcgtatt acgttcgtta ttaactgctg gggtgctggc
    31081 gtcgggcctg ttgtggagcc tgaacggaat taccgctacc cctgcggcgc aggcatctgg
    31141 tgatcgctac gaagttaccc agcagcgtaa cccggatgcc gcctgtctgg actgtcataa
    31201 gccagatatc gaaggtatgc atggcaaaca tgcttccgtt atcaacccga ataacaaact
    31261 gccggtcacc tgcaccaact gtcacggcca gccatcaccg caacaccgcg aaggggtgaa
    31321 agatgtgatg cgctttaacg agccgatgta caaggtcgga gagcagaaca gcgtctgtat
    31381 gtcctgtcat ctgccggaac agttgcaaaa agcgttctgg ccgcacgatg tccacgtaac
    31441 caaagtggcg tgcgccagct gccattccct gcatccacag caagatacga tgcagacgtt
    31501 aagtgacaaa ggacggatta agatttgcgt cgattgccac agcgatcagc gcaccaatcc
    31561 gaactttaac ccggcgtctg ttccgttgct taaggagcag ccatgacctg gtctcgtcgc
    31621 cagtttctca ccggcgtcgg cgtgctggca gccgtcagcg gcaccgcagg gcgcgtggtg
    31681 gcgaaaacgt tgaatatcaa tggggtgcgt tacggcatgg tgcatgacga gtcgttatgc
    31741 atcggctgta ccgcctgtat ggatgcttgt cgggaagtga acaaggtgcc ggaaggcgtc
    31801 tcgcgcctga tgattattcg tagcgagccg cagggcgaat ttcctgatgt gaaatatcgc
    31861 ttcttccgta agtcttgcca gcactgcgat catgcgccgt gcgttgacgt ctgcccgacc
    31921 ggtgcgtctt ttcgtgatgc cgctagcggg attgtcgatg ttaatcctga tctctgcgtc
    31981 ggttgtcagt actgcatcgc cgcctgtccg taccgcgtgc gctttatcca tccggtcacg
    32041 aagacggcgg acaaatgcga tttctgccgt aagaccaatt tgcaggccgg taagctgccc
    32101 gcgtgcgttg aggcttgccc gacgaaggcg ctgacgtttg gcaatctgga cgatcctaac
    32161 agtgagattt cgcaactgct gcgccagaag cctacttacc gctacaagct ggcgctggga
    32221 accaaaccga agctgtaccg cgtaccgttt aaatacgggg aggtgagcca atgacgcaga
    32281 cttccgcatt tcattttgaa tcgctggtgt gggactggcc gattgccatc tacctgtttt
    32341 tgattggtat ttctgccggt ctggtgacgc tggccgtgct gttacgtcgc ttctacccgc
    32401 aggcgggcgg tgcagacagc acgttgctgc gcaccacgct gattgtcgga cctggcgcgg
    32461 tgatcctcgg tctgttgatc ctcgtcttcc acctgacaag accgtggacc ttctggaagc
    32521 tgatgttcca ctacagtttt acctcggtga tgtcgatggg ggtgatgctg tttcagctct
    32581 acatggtggt gctggtgctg tggctggcga aaatctttga acatgatttg cttgccctgc
    32641 aacaacgctg gttgccgaag ctggggatcg tgcaaaaggt tctgagcctg ctgacgcccg
    32701 ttcatcgcgg actggaaacg ctgatgctgg tgttggcggt gctgctgggg gcttataccg
    32761 gctttctgct gtcggcgctg aaatcgtatc cgttcctcaa taacccgatc ctgccggtgc
    32821 tgttcctctt ctccggcatc tcgtccggtg tggcggtagc gctgatcgcc atggcgatac
    32881 gccaacgcaa aaatccgcat tccacggaag cgcactttgt tcaccgtatg gaaatccccg
    32941 tggtatgggg tgaaatcttc ctgctggtgg cgttttttgt cggtctggcg ctgggcgatg
    33001 acggtaaagt gcgtgcgctg gtggcggcat taggtggcgg tttctggacg tggtggttct
    33061 ggcttggtgt cgccgggctg gggctgattg tgccaatgtt gctcaaaccg tgggtcaatc
    33121 gcagttccgg cattcctgcc gtgttggcgg cgtgtggggc cagtctggtc ggcgtgttga
    33181 tgctgcgctt tttcattctc tacgccggac agttgacggt ggcgtaagcc agaaaagagg
    33241 tggtttctgg acgtattcct tcctgaagtc ggttttctgg cgttgttgtt aagtctcggg
    33301 gtcaacgtgt tgaccccgtt gacggccttt gcgggagtgc ggttgcgctg gcctgccatg
    33361 atgcgactca cttgcatcgg cattctggcg cagttcgcgc tcctgctgct cgcctttggc
    33421 gtactgacgt attgttttct catcagcgat ttctcggtca tttatgtcgc gcaacatagc
    33481 tatagcctgc tgtcgtggga actcaagctg gcagcggtgt ggggcggcca tgaaggttcg
    33541 ctgctgcttt gggtgctgct gctttccgcc tggagcgcgc tgtttgcctg gcattatcgg
    33601 cagcaaaccg atccgctatt tccgctgacg ttagccgttt tatctctcat gctcgccgca
    33661 ctgctactgt ttgtggtgct gtggtccgat cccttcgtgc ggatatttcc accagcaatc
    33721 gaaggccgcg atctcaatcc gatgctgcaa catcccggtc ttatctttca tccaccgctg
    33781 ctttaccttg gctatggcgg tttgatggta gcggcgagcg tggcgctggc gagtttactg
    33841 cgcggcgagt ttgatggtgc ctgcgccaga atttgttggc gctgggcact acctggctgg
    33901 agcgcattaa cggcggggat catcctcggt tcctggtggg cctattgcga actgggctgg
    33961 ggcggctggt ggttctggga tccggtggaa aacgcctctt tattaccctg gctttctgcc
    34021 actgcgctgc tgcacagttt gtccctgaca cgccagcggg agattttccg ccactggtcg
    34081 ctgttgctgg cgatagtaac tctgatgctg tcgctactgg gtaccttaat tgtccgttct
    34141 ggcattctgg tttcggttca tgcgttcgca ctggataacg tccgcgccgt gccgttgttc
    34201 agcctgtttg cactgattag ccttgcgtct ctggctctgt atggctggcg agcgcgggac
    34261 ggtggcccgg tggtgcgttt ttcggggtta tcgcgggaaa tgttaatcct cgctacgctg
    34321 ttgctgtttt gcgcagtgct actgatcgtg ctggtgggaa cgctttatcc gatgatttac
    34381 ggtctgctgg gctggggacg cctctccgtt ggcgcgccgt attttaaccg cgcgacgtta
    34441 ccgtttggcc tgttgatgct ggtggtgatt gtgctggcga cgtttgtctc tggcaaacgc
    34501 gtgcagcttc cggcgctggt agctcatgcg ggcgtgctgt tatttgccgc tgggatcgtg
    34561 gtttccagcg tcagccgtca ggagatcagc ctgaatttac agccgggtca gcaggtgacg
    34621 ctggcaggat acaccttccg ttttgagcgc ctcgatctgc aagccaaagg caattacacc
    34681 agcgaaaaag cgatagtggc actgtttgac catcagcaac gcattggtga actgatgccg
    34741 gagcggcgtt tttacgaagc acgtcgtcag caaatgatgg aaccgtcaat tcgctggaac
    34801 ggcatccatg actggtatgc ggtcatgggt gaaaaaaccg gagcggatcg ttacgctttt
    34861 cgcttgtatg tacaaagcgg tgtgcgctgg atctgggggg gaggattgtt gatgattgcg
    34921 ggcgcattat taagcggatg gcgggggagg aagcgcgatg aataaaggac ttctcacgct
    34981 actgctgtta tttacctgtt ttgcgcgcgc tcaggttgta gacacctggc aattcgccaa
    35041 tccgcaacaa cagcaacagg cgttaaatat tgccagccag ttacgttgtc cgcagtgcca
    35101 gaatcagaac ttactggaat ccaatgcacc ggtggcggtc agtatgcgcc atcaggttta
    35161 cagcatggtg gcggagggga agaacgaagt cgaaatcatt ggctggatga ccgaacgcta
    35221 cggagatttt gttcgctata acccaccgtt aacgggtcag acgctggtgt tatgggcgct
    35281 gccagtggtg ttgttactgc tgatggcact gatcctctgg cgagtgaggg cgaagcggtg
    35341 aaacagtcac aaataccggt gaaaatgctg acaacactca cgattttgat ggtatttctc
    35401 tgtgtcggca gttatctgtt aagcccaaaa tggcaggcgg tacgtgcgca atatcagcgt
    35461 cagcgcgatc cgctacatca gtttgccagc cagcaaaccc cggaagcgca gcttcaggta
    35521 ttgcaggata aaatccgcgc taatccacaa aacagcgaac agtgggcgtt actgggcgag
    35581 tactatctgt ggcaaaacga ttacagtaat tcgctgctgg cgtatcgtca ggcgttgcaa
    35641 ctacgtggtg agaacgctga actgtatgcg gcgctggcga cggtgcttta ttaccaggcc
    35701 agccagcata tgaccgccca gactcgcgca atgatcgaca aagccctcgc gctggactct
    35761 aacgaaatca ccgctctgat gctgctggct tccgatgcgt ttatgcaggc gaattacgcg
    35821 caagccatcg aactatggca aaaagtgatg gatctcaact caccccggat caaccggaca
    35881 cagctggtcg agtcgattaa tatggcgaaa ttgttgcagc ggagatcgga ttaatcatct
    35941 ggtgatgaag attatgttat tgagctgcat aaaaaataat cgaatgaaca tatgccaaaa
    36001 ataatcacca atcagtatta ttgcagatta aacaaataaa aatctttcca taacaaatgg
    36061 ttattcatta atcctgccat aaactcgcgt tattatgcat taatgcagcg aaaagctctg
    36121 ttgttaaagg gttgcgcaac ataccgcgca aatgatactg atcataagcg ttaaaaaaat
    36181 ctacaaacca acgcaacaca attcatgccc tggcagtatg tcacgttctc gcgtttctga
    36241 acggggaacg gcgctccatt gaggaagtta ttctgatgaa aaatataaaa ttcagcctgg
    36301 cctggcagat tctgtttgct atggtgctgg gcattctcct gggaagctac ctgcactacc
    36361 atagcgacag ccgcgactgg ctggtcgtca atttgctctc tccggcgggt gatatcttca
    36421 tccatctgat taaaatgata gttgtgccga ttgtgatctc cacgctggtg gtgggtatcg
    36481 cgggtgttgg tgatgccaaa cagctcgggc gtattggcgc gaaaaccatt atctacttcg
    36541 aggtgatcac caccatcgct atcattttgg ggatcactct ggcgaacgtc ttccagcccg
    36601 gtgccggggt ggatatgtcg cagctggcga ccgtcgatat ctcgaaatat cagagcacta
    36661 cggaagcggt acaaagcagt tcccacggca ttatgggcac gattttgtcg ctggtgccga
    36721 cgaacattgt ggcgtcgatg gcgaaaggcg aaatgctgcc gatcatcttc ttctcggtgc
    36781 tgtttggtct ggggctttcc tccctgcccg cgacgcatcg tgaaccgctg gtgaccgtgt
    36841 tccgctccat ctctgaaacc atgtttaaag tgactcacat ggtgatgcgt tatgcaccgg
    36901 tgggtgtgtt tgcgctgatt gcggtgacgg tggctaactt tggtttctct tctctgtggc
    36961 cactggcgaa actggtgctg ctggtgcatt tcgccattct gttcttcgcg ctggtagtgc
    37021 tgggaattgt ggcgcgcctg tgcgggttaa gcgtctggat cctgattcgt attctgaaag
    37081 atgagctgat tctggcgtac tccactgcca gctctgaaag cgtactgccg cgaattattg
    37141 agaagatgga agcctacgga gcaccggcgt cgatcaccag cttcgtggtg ccgaccggtt
    37201 actcttttaa ccttgatggt tcgacgctgt atcaaagtat tgccgctatt ttcatcgctc
    37261 agctgtatgg cattgacctg tccatctggc aggaaatcat tctggtgctg acgctgatgg
    37321 tgacctcgaa agggattgct ggcgtgccgg gcgtgtcgtt tgtggtgttg ctggcaacgc
    37381 tgggtagcgt aggtattccg ctggaaggcc tggcgtttat tgctggtgtt gaccgtatcc
    37441 tcgacatggc gcgtactgcg ctgaacgtgg tgggtaatgc gctggcggtg ctggtgattg
    37501 ccaagtggga acacaaattt gaccgcaaaa aggcgctggc ttatgagcgt gaagtgctgg
    37561 gcaaatttga taaaactgcg gatcaataat tgaagattgc cggggttatc cacccggcaa
    37621 tgtgtggatg cctgatgcga cgctggtgcg tcttatcagg cctaccttaa ccgttggtta
    37681 atttttcaaa ctcttcacac ccggtatcaa acccttccat acagctcagg ttcaaccagt
    37741 gcagcgcctt ttgtttgttc ttctcgataa aaccttcctc accgtttaag aacatcattc
    37801 ccgcccagta ctcggaataa ccggttcggg aaattgcaga gctgcgtttg aaataccagg
    37861 ttgccttgtc atcgtcggcc ttaatgccca cgccgttggc gtaaatcaaa ccaagcagca
    37921 tttgggcatc gacggcagag tcgttctcca gatcttccga ggcgttttct aacagcgaaa
    37981 tcgcctttgg ataatccggt ttacccgctt gagtatttac cagaatatgc gccagcgtta
    38041 cttcaccttc tttactgccc gcttgcgccg ctttctctgc aagaactttt gcttgtggat
    38101 agtccagact gaccggattg gtgattttga tctgcgccag cagcgcgcag gcatcagcat
    38161 caccgctgtc ggcggctttc tgtgcccaat attcggcttt gctcaaatcg ccggagctaa
    38221 accagctgtc ggcaagaaaa tattgtgcgc gtcggtcccc ggcctctgct gcctttaaat
    38281 actggctgcc aggctcggag tcgttcgcgt gggcaaagaa tgtcaaaaac aacatcaatg
    38341 cgataatttt tttcattttg ataatacgat tgggtaggga gtaaccagta taaagagttc
    38401 gaatggataa aaaaacagcc tccgaaagga ggctgtagaa aggactgtat tacgccagtg
    38461 ccgcttcgcg caggcgagtt ttcaacttgt tgtactcgtc aatcacgtac tgctcggcgg
    38521 cgcgctgatc ggcgatcggc tcgacgcgca cggcgcagta tttgtattcc ggcgttttcg
    38581 taatcgggct taagttttcg gtaaccagct cgttacaggc accaatccac cactggtagg
    38641 tcatgtaaat cgcccctttg ttcggacgat cgctgacctg cgcacgggtg ataattttgc
    38701 ctttacgcga gtgcacccaa accaatgcct catcttcaat acccagacgt ttggcgtctt
    38761 cggtattgat ttgtgcgtag ccaggttcat cagccagcgc cgccagtgcc gcacagttac
    38821 cggtcatcga acggcaagag tagtgaccaa cttcacgcac cgttgacagt accatcgggt
    38881 actcgtcggt gagtttgtcg attggcgcta cccagtcgca ggtgaagaac tgcgccagac
    38941 cgttcggggt atcaaacttc tctttaaaca gataagaagt cccctgatcg gcatctgaag
    39001 tatcgcggca aggccactga atgaagccca gttcgcccat tttctcgtaa gtcgcaccgt
    39061 agaaatccgg gcacagatga cgcaactcat cccagatctc ctgggtgttg ttgtagtgca
    39121 tcggataacc catacgggtg gcgatttcac tgatgatttg ccagtccgtt ttcagatccc
    39181 atttcggttc aactgctttg aagaaacgct ggaagccacg gtcagccgca gtaaacacgc
    39241 cttcatgctc gccccacgac gttgacggta aaataacatc cgccgccgac gcggttttgg
    39301 tcataaagat gtcctgaacg ataaccagtt ccagatcttc aaaggcttta cgtactgccg
    39361 acagctccgc gtcagtttgc agcggatctt cgcccataat gtacgcggca cgcacttcgc
    39421 catgcgctgc gcggtgcggc agctcgctga tgcgataacc ggtatgagca ggcaggcttt
    39481 ccacgcccca ggctttggcg aatttctcgc ggttagccgg atctttcacg tactggtatc
    39541 ccggatacgt atccggcagc gcgcccatat cgcaggcacc ctgcacgttg ttctgaccac
    39601 gaaccgggtt tacacccgca tgcggcttac cgaggttacc ggtcagcatc gcgaggctgg
    39661 tcagagaacg cacggtttcc acgccctggt agaactgggt tacacccatg ccccacagga
    39721 tggcggcgct tttcgcctgg gcatacatcc gtgccgcctg acgaatctca ctggcgctga
    39781 cgccggtgat atcttcaacc gactccggcg tgtagccttc aacgatttta cgatactctt
    39841 caaagccttc tgtacgggaa gcgacgaacg ctttgtcgta cagattttct tcaataatga
    39901 catggcccat cgcattcaac agcgcgatgt tcgagccgtt tttcagtgca atgtgcatgt
    39961 cagcaatgcg cgcggtttca attttgcgcg gatcgcagac gataattttc gccccgttac
    40021 gtttagcgtt aattacgtga ttcgccacga ttgggtggga atccgccggg ttgtacccga
    40081 aaacgaacac taaatcggta ttatcaattt cgttaatagc attgctcatt gcgccattac
    40141 cgaccgattg gtgcagacct gcaaccgatg ggccgtgtca gacacgagcg cagcagtcaa
    40201 cgttattggt accaataacg gcgcgcgcaa atttttgcat tacatagttg gtttcgttac
    40261 ccgtaccacg cgaggagccg gtcgtctgga tggcatccgg accgtacttc tctttgatgg
    40321 cgctcaggcg ctcggcaacg taattcagtg cctcatccca ggaaacaggt tcgagtttgc
    40381 cgccacgctg gcgacggatc atgggggttt tcaggcgcgg ggtcaggatc tgggtatcgt
    40441 taatgaaatc ccagccataa taacccttca gacacagggt accctggttg gttttcccct
    40501 gcgctgcctc cgcccggacg attttgccgt tatcgacgac caggttgatt ttgcaacctg
    40561 atgcgcaata ggggcaaacc gtgacgactt ttttcatcgg tctcgctcca gttaatcaaa
    40621 tcacgcatac gcgctctcga ctacagtatg catcttttat gccacatttt atttggggtc
    40681 attccctgat attacgggca ctatttattc aaaactctga cgaaaaacag gctgtcgtca
    40741 gttttgacgt gacgaaacga aataccgcgt gacagccatc acgcggcaga cattttattt
    40801 tttctcgacg acgggagccg cctgataccc gccgcccagc gatttcatca actgaatgct
    40861 ttggatcacc cggcggctgt ccagcatcag taatgacatc tcttcggcaa gtactggcaa
    40921 ccgggcttca gtggcctgta agcggctggt taagccgcgc tgataggcgg cctcggcagc
    40981 gcgctgggtg aagcgcgttg cttccacgcg ttcagcctgc atttctcgct cgtcgttgag
    41041 cgtttgcaga cgcgtgccgt tgacggcaac gtcacgcacc gcgttcagta ctgactggtt
    41101 gtaacgttca atcatcatgt tgctggcggc gcgcgtgcct tcgagattgg cattcaaccg
    41161 tccaccgtca aacagcggca atttcagacc cgggatgaag ttgaactggc gactggtttt
    41221 tttgaataag gtatccaggt ggatggcatc aaggccgaaa aacgctttga tatcaaagct
    41281 cgggtagaac agcgcccgcg cggaatccac ctgatctaat gacgcctgaa cataccagcg
    41341 catggcttgc agatccgggc gtctggcgag caactcataa gagagtgtcg ccggaatgcc
    41401 ggtctggact cgtggtaatg ccaccggttt gatctccggc atatcgctgg cccccgcgcc
    41461 aatcaatgca cgcagagatt ctcgcgtttc ggtgatttgc cctttgacgg cagcaatttg
    41521 tttatcgacc gccagaatct gtgcccgcgc gccgtggaaa ggcacttgtg cttccagacc
    41581 gtgcgccact ttactctggt gcgctttcac cgcgtaatca atcacatcgc gagtttgttc
    41641 taacagatcg agcatctgat agctggcctg catactgtaa taaagctgcg ctacgcccgt
    41701 ggtcagcgat agctctactg ctgcggtttc tgccagcgcg gcattatgcg cgccaatggc
    41761 ggcggcaacc gctgagcgat gcacacccca caaatcaaga tccagtccgg caaacaaacc
    41821 tactgtggct tccgtatagt acggcccgtc catacccagt gcgggcgcat ccatcgcata
    41881 agggcttaaa aagccgttcg ccgagacgcg ttggcggttg agcatcccta acgctgcgac
    41941 ctgtaattgt gaaccggcat ctaacaaatc ggcctgggac tgcgcttttt cttcccgcag
    42001 tttcgcttcg gcgagggtgt gtgaaccact tagcgtccgt tggatcagcg catccagctg
    42061 cgggtcattg agttgtttcc accactgcgc ctgcggccag ccggagctgg caagatgaat
    42121 atcgtcggcc agtttgattt gttccggttt gagctgttga tgaggtgcag aatccttacg
    42181 taccagggca cagccggaaa tcagcgtcgt gctgccgaga atgctgcaca acagcagacg
    42241 tgaaagttga cgattgatca ttgcgtggct ccttgtgcct gttccgtggc gggcgttaat
    42301 gccggggctg tccagtctgg caagcgggcg aaaagctggc agacgtgttg ctcctgtttt
    42361 aataaggtgg gcaggatggc ctgcgaggcg ggtgtctctt caagcgttat ttgaggagaa
    42421 cggctgagtg cggttgccag accggcagcg tatttctcca gggcgtcggc aaactgagct
    42481 gctctgtccg gctgtagtgc gttatccagc gcctgcgccg agttccaggt ggcatcccag
    42541 gcgtgaagaa tatcgcggcc ctgacgaata accgtttgcg aacgttcaat cagtaaggcg
    42601 cgttcttcgc tgtccagttg acgctccagc gccacgcgtt ggcacatctc ttcacaggca
    42661 ttaaacgccg catgcagacc gatacgaatt tgcagataag tgcgcagagc cgtgacttcc
    42721 tgctggcgtg ggatccgcat tactttactt aacataccca gcgtgccagc cagtttttgc
    42781 ggcagtgtgc gcgcttcact ttcaggccag acaaaggtgt aaatcaccgc ggacaccacg
    42841 gtaccaatga tgatacccat ggcgcgatcg cgaatttcca ccaggtcgta caccgggcca
    42901 aaaacgtttt cgagcgtggc gagcgcgaag gtgaccacca tctgtgtgcc gatataagaa
    42961 gagcgttcag agctggtggc aatccatgcg cccaacagga aaatcggtgc cagcacaaac
    43021 agcaattcga caatattgtc cagccagggc atgaccagta gcgtgaataa cagcgccaga
    43081 atcgcgccgc aaaaggcccc gccaaaacgc agcaccatct tctggtacga cgaaccgaca
    43141 tttgggttag cgacgatcac gcatgtcagc atacaggtgt gaatgccttc ccagtccacg
    43201 ccgctgtaga aggtgtaaca gatcaaacag gcgagcagtg tttttaccgc gtagcgcata
    43261 tagtctggat tggtaaaagc atcggcgacc attgatggcg gtttggcggc gggcgttggc
    43321 ggcgtattcg ggtccatctg acccagttgt aacaacgtct ggcagatatt ctccaggtta
    43381 cattcccgtg ccgccatcgc ttcactttca gtgatccgcc agtcgctttg ccagcactga
    43441 ccttcagcaa cggcatgctg cagcttattg atttctgagg ctaatttttg ccgaaattca
    43501 ataattgcct gagaatcagc aaaagaggtg ggatcgtagc gattcagcgt cgagtaaatg
    43561 taggttaccg ttgccacgca gctttgccac catgcgcttt gagttcgcca gttggcatcg
    43621 tccgcgagac aaaagacatt cagtttttgc agcgccagcg cctctctttc aatccgcgtt
    43681 tcgggtagcg gtgcgaggct gtccgtcagg tgactaatgg catcatcaag ccgatcatta
    43741 agcgcttgat gcatttgcgt aatggcacga ctgggaaacc acagcacgcc gattaacgtc
    43801 atcagcaagg ttggatagag gccaacaacg atacaccaca gcgttaagcg cacgaccact
    43861 tccggatagt cgagcatggc ggggaaggtt tgcccgtaaa tggcgacaat ggcgacagcg
    43921 aaaaagacca gtcccagccg atgggtgcgc atcaaaaaca tacagcccat caggatcggc
    43981 ccggcgatga tcaatcggat caacggttcg ccgtatgacc atttgtagat caaaaacagg
    44041 ctgccgatct ccagcgccgt ggcaaccaca aacaagatcg cgacaaattt ggtgtaaaac
    44101 gcgttcgact gaataccgta aaacagcact gccagcgata acgccacaaa agggatctca
    44161 aaagtcatcg agatcagaat caccagcagg cagcctaccc agagttgcac gatctgcggc
    44221 acgcggccgg gtcgccgctc gcttaactct tcatgaaaga acgccagcag cctgaccacc
    44281 ggtaatggca gggagttgag cgcgctcatt attgcggctc aagattagcg actgccgaag
    44341 cgccgatgcg gaacatttcc gggtcaggtt tatcgaccat gattttgacc ggaaaacgct
    44401 gggcaacgcg gacccagtta atagagcgag aaactttcgg caggccgccc agcaccaggc
    44461 cgccgtcatc cggtagcacg ccgtagccaa tcgaatccac tttaccctcg aaggttttgc
    44521 cgctgtcact catcaggcga atcgttgcgg gtgtacctga gcgaatattt ttcagatcgg
    44581 tttcgcggaa gttggcgatc acgtaccagt gacgagtgtc gattagggta aaaatagggc
    44641 gcatggcaga agcaaattgc ccgacggagg ttttgaggga aatgacccgg ccatcaaacg
    44701 gcgcgcgaac ggtcgccatt tccagatgaa gtttggtcag ggcaatatcc gcttcgaccg
    44761 ccgcacgttg cgccaccagc gcatcgacgc cgctgacggc gctggcggct gactgcgcct
    44821 gtaacaatac ggcattaaga tcggcttctg cggcgcgctg cgcagttctt gcacggtcaa
    44881 cgtcttccgc tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg
    44941 tggcctgttt cgcggcggca cgggcttttt ctaccgtggc attaatcgag tcggcaccaa
    45001 actgttgcgc atcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg
    45061 aggcttcagc tttcgccaga ttggcttcgt acgggcgcgg gtcaatgcgg aacagcaaat
    45121 cgccctgttt gactgcctgg ttgtcggtga ccgccagttc tacaatgcgg ccgctgactt
    45181 ccggcaccac atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt
    45241 ctacgcgcca gataacgaaa acaagggcaa ccagcgccaa tgcaaccact aacagagcag
    45301 ggaatttact gcgaggagct tttttcggcg tactttccat gagcgcgtct caaatagatt
    45361 agaaaaatgc cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc
    45421 cgacaaatgc cagattgata tttgcccgct gaataactcg cctcgtgatg agcatcaaaa
    45481 taaggctggc aatggcgcag aaaaaccagc tgggataata agcaccgatc accgggattg
    45541 ccggagataa tgagcatccc gaaaccatga agacgggaat tatccaggct gtttttttga
    45601 gttgcatcgc catgcgagag agaactgttg gcatattaaa tacatcctgt ctgctgcgaa
    45661 caatatctgt agtgaatatc aatatccatt gaaggcgtca gtatggctat tttctcgatg
    45721 aataaaaata gctttattca gccaccgcat tcataactta tatattttat ttattaattg
    45781 gctgtatata tttttaaaat aaatgctttt gggggcttca gcgcccccaa aagcgagtga
    45841 cattaatttg gggttacgat attcacccac aaatcgaaat tatccagaca ggcgataatt
    45901 tcttccagtt tcgcgccatt gccaataatt ttggctttct tcgcttttac cagatccgcc
    45961 attttggctt gcccggtcag cacggcgtgc agatcttcac ggctgatgta gaaagaggcg
    46021 tcagcttggg attgcagtgt tttacggtag ttaagcacgc tatcgttcag cgtcaggttg
    46081 aggttatcgc cattgctcat attgaagttc aggctgatat ttttacccgc ggctttcgcg
    46141 ctatcgaggc gaacggacat aaagtcgaac agcatttcga ccgacatccc gcgaatggtg
    46201 tccggggaac cggtggtgcc gtggctgaac ttatgcaccc cttcgcgcag ctctttcgcg
    46261 ccggtcaggt agaaaccgcg ccaggtggcg gactcggcct gatagcccag ctgttcaaag
    46321 ttattcgctt gcagattctt cgcgacctgg tcacccgggt tggcggcaat cacctgtttc
    46381 agcagttctg ccgaccagcg gtaatcacct tgcttgttcg cttcttgcgc caggttgatg
    46441 acacgggcag aaccgcccag cgcctgcacg taacgtttac ccatctccac ctgaccatac
    46501 ggatgcaggt tagccgggtt accgtcgtaa tagccaagat agaagttata caccgcgcgg
    46561 gcgttgtggc tgacagaacc gtaatagcca cggctggccc agttattggc aagtgcaggc
    46621 ggcagtttaa tcatgtcgcc gatttcattc atggtgtagc cctggttcgc caggtgcagg
    46681 gtctggtcgt gaatgtactt gatagtatcg cgatatttac caatataatc attgatatgc
    46741 ttattgcccc agaccggcca ggtgtgcggc ataaacagga cttccgcgtc gttaccccac
    46801 atatccagcg tttcgttcag atactcggtc cacttgctgg tgtcgcgggt tttcgcgccg
    46861 cgtagagtgt agaagttgtg cagggtatgc gtggcgttct cggcggtaca cagcgctttc
    46921 agggccggaa tatagaagtg catttcggct ggtgcttcgc tacctggggt catcaggaag
    46981 tcaaactcca ggccgtcgat aatcatcttc tcaccggttt tggtgatggt tttgttcggt
    47041 gcgatgatgc tcgggtagcc cgatgccagc gttacgccaa gaccattgcc gatgttaccc
    47101 tgagggttat gcgccagcag caggccgtag gagtaaagtg cacggcggct cataatatta
    47161 cccgccagta cgttttcgct gatggcttcg tccataaagc cagcaggggc gataacctgc
    47221 actttaccgg atttcacatc ggcttcagag ataatgcctt tcacgccgcc atagtggtcg
    47281 gtatggctgt gggtataaat aaccgcaaca atcggttttt gcgggcgatt ttggaagtaa
    47341 aggtcaaggg cggcttttgc gctcggtggc gtcaccagcg ggtcgatgac aataatcccg
    47401 gtatcacctt cgataaaggt gatgttggag atatcctgac cgcgaacctg gtacattctg
    47461 tcggtgactt taaacaggcc ggaaataccg tttaactgcg actgacgcca caggctaggg
    47521 ttaacggttt cgggcgcggg cgcattgata tcaaatttat aatcttcccc gcgataatat
    47581 ggtttgccat tagcatcgcg cagaatacct tcatccagca gcggagcgat aaatccacgc
    47641 tgagcgtcgt caaaatcctg acgatcgcta aacggtaatg atttggcgta attctgatta
    47701 acctgttggg taatattgag tggcggcttt cgcttcatct gttgcgtaag cggtatttac
    47761 catcatgccg ctggcggcag ttaacgcaat aataattctg ctcaaacgga ataaccgaga
    47821 gttattcata taaatctcca tttatcattt atgtcgtaaa tatgtaatcc atgcaaaata
    47881 cgtcatttaa agctggggtt atcataattg ggaatttgcg actttaataa gtggaagtgt
    47941 gagctgaacg atccatttta ttaggcattt gtgctgaaga ggaaaagtta cagtaaaact
    48001 atcattgcag aaaataatat gagccgtttt atgtgtttat tatatatatt gtacctgtcg
    48061 ttggcacagc gggacattgc gctactgtgc caacgcttta ctcatggggc tttagcacag
    48121 gactgtggca aaaaatgttg atgcgccaat attgctgcac cctgagcgcc attaaagtca
    48181 gaagatgagg cggcaataaa gcgcacgacc tgatgcggca gtggacggcg caggtacttt
    48241 tgggtcatgg caatgagagt ctcgcgtggg aaggcgggca tatccatcac gccaccgccc
    48301 agaatcaccg catcgggatc gaacagatta atgctggtgg caatggcccg tgccgcgttt
    48361 tcaagcagac tctggacgaa aggggcgttt tccgcatgga cgaaaaggtc gctcaatggg
    48421 taatttcggg gctgttgttc gtaccagcgt cttagcgcca ttccagagca attggtttcc
    48481 aggcacccag gattgccaca cgcgcagtgt tgggtcatat ctcccagggg gatatgaccc
    48541 agttcgcctg ccacaccgtg tgcacccgtc cacggcgcac cgttcatcca cactgcgaac
    48601 cccatccccg taccgagata ggccgccaga accagttgtt gcgtaaggcg gttttctact
    48661 acgtcccagg agagttgcag gttaacgtcg cgggaaaact caaccggaca attcagcgta
    48721 ttttcgagct tatcggcgag atcatataaa tccgccgctg ttaacggcag gttaggcgta
    48781 gaaattatgg tgcgtttatc tttactgacc agcgccggaa atcccatcac cagaccatga
    48841 cagcgagcgt taaagcgcct gagttgctcg tcaatcattt cgccgatacc cgacaccagg
    48901 ccgggagcaa tgacttctgc ggtccgcttt ttttcgcagt gtagcgtttc accttctgct
    48961 gtccgcagac aaaagcggat atgcgttgcc cccatatcca cgcccgctac gacgttatgc
    49021 tgtttttgca tgaggctgta cctcgctttt tgcagccaga atctgcgtgg tcataattct
    49081 ccatgcttcg tcgatatttt ccgcatgatt aaacaggccg gaagtgccga cgataaagac
    49141 atccgcccct gccgccatca gtttttcgta agttgcctgg ttgcaggaac cgtccacctc
    49201 aatttcgtac tccagacctt ctcgttcacg ccatgccttc agttcggcaa gtttatccag
    49261 catttcagga atgaacggtt gtccggcaaa gccgggatcg acagtcatga ccgtaatttt
    49321 atcggcctta tggatatagt atttcatggc ctcaactggt gtctccgggt taaggatcag
    49381 ccccactttc atgtcatgac agcggatttc atcaatcagg cggaacgcct gaccgttgat
    49441 agtttccgga tgcaaagtga tgaaatcagc tcccgcacgc gccagttgag caatgtaatc
    49501 ctgcggccgc gtcaccatca gatgacagtc gagcggttta gttgccagtt ttttaacctg
    49561 acttacgaag aacggtgaga gtgtcagatt ggggacaaag tgaccatcca tgatatcgat
    49621 gtgaaagtaa tcggcatggc tgtcgataaa ttcgatctgt tctttaaatt tcagcagatc
    49681 catacacatt aacgaggggg agattttcat gttcaattcc ttacttactg ataagacggt
    49741 caagggcgac agccgcgata attaatccgc ccatcaccac cagttggtaa taggtttgta
    49801 cctgcaaaat attcagaccg ttgttgatgg tgccgatgat caacccgcca atcaccacag
    49861 agaaaatgcg ccccttgccg ccgaagaaac tggtgccgcc aatgatggcg ctggcaatgg
    49921 cataggtttc aaaacccata ccggcaagcg gttctgcggc accgagtcgt gcagttgaga
    49981 cgacgcctgc cagacctgca caaacaccag agatgataaa caccaccagg atgtggaatt
    50041 tcacgtcaat cccggaatag aacgccgaat ttttgttgcc gcccagtgcg tagatgttgc
    50101 gcccgagccg catacgcgtt gtcagaaacc aaaggatgag cgcgacaatt agcgagaaga
    50161 taacggggac aggtatccca attacgctgg cggcaaagaa gttcacgaag tcaaaggaga
    50221 agccgtatac cgagttggca tcggagatca ccagcgtgat cccacggaaa atcgcgttgg
    50281 tgccaagggt gatgatgaac gggtgtagcc ccgtccagtt gaccaggcag ccgttgatcg
    50341 cccccagtgc gccgccaacc agtacaccgc caatcatcgc tgcgagaaac gggtcaacac
    50401 ctgccaacat cagtttggcg gtcaccatac cggaaagcgc cagaatcgcg ccaaccgaga
    50461 ggtcgatacc agcgaccagg atagcgaaaa actcgcccat gccgatcaat accgtcacgg
    50521 agctttgaac aaaaatctgg gtaatattat tggtggtcag aaaatattct ggcgacagcg
    50581 aaccaaaaat ggcgacgatg atcgccagga taaaaaaggt gccgtattta tcccagaaca
    50641 gcgcaaagtt gaacggtttc ttctcgctcg cttcgctttt tactcttgtg gtaaagccca
    50701 tgccataatc tcctcttcgc tcatgtcatc gcgattcgtc aggatttgcg tcagtcgtcc
    50761 ttcgcagaac acggcgatgc ggtcgcagac ggtgataatt tcaggtagtt cagatgacac
    50821 catcaggatg acttttccgt cgtccgccag ttggcgcatc actttgtaaa tttcggcttt
    50881 cgcgccaacg tcgatgccgc gggtaggttc atcgaaaata atcacttccg ggcaacagca
    50941 cagccatttg gagatcagga ctttctgctg atttcccccg gagagttcgg tgatattctg
    51001 gtttaccgaa tgacatttca gcgccagcag ttcgcgttga ttttcagcgg tacgttgctc
    51061 gtcaacttca tgaaacaagc ccatcgcgcc tttatagccg ccgtctttca gactgcggct
    51121 gatcgccatg ttctgagcga tggaaaagtt gggaaaaaaa ccgttatccc ggcggctttc
    51181 agtgatgtaa gccatccctt ttttcactgc atccaggggg gaacgtggag agatatcttt
    51241 gccattaaga cggatttctc cgccagcgcg tttatcaacg ccaaacagac aattcatcag
    51301 ttcagtacgt ccggaaccga ccagcccggc aaagcctaat attctccccg gcagacgcta
    51361 aatgagatat cccggacctt ttttctgtca cggctggtga cgttgcgcac ctcaaaaacc
    51421 gtatcgtgca caaggttgct gacattctcc ttcatcgcgt taaaacggtt ttgcagttcg
    51481 cggcctacca tcagacggac gatatcgtca tttgacacat cgcttaccat gccgctgcaa
    51541 acgctgctgc cgtctttcat caccgtatag cggtcgcaaa tacggcgaat ttccgccaac
    51601 ttatgcgaga tatagacgat ggccgtaccc tctttacgca actgattcat gatcagaaac
    51661 agatagtcca cctctttatt ggtgagtgag gaggtgggtt catccatgat gatgactttg
    51721 gcatcgagca tcagcgtttt ggcaatttcc agcatctgct tgtggctgat agatagattc
    51781 gccactttct catctaaatc aactttcaag cccacgcgta ataacatcat ggcggcgcgg
    51841 acacgcattt ctcgccagtc gataatattg acgccacaga tttttttcgt cagatgacga
    51901 ccaatatata aattttccag tacggttaat tcatcaataa cgctgagttc ctgataaata
    51961 atcccgatac cgagttgtgc cgctaattta tgatccagct tgttatagct aatgttatta
    52021 atggtaatgg tgcctttggt cggctcatgt attccggata aaaccttcat tagcgtggat
    52081 ttacccgcgc cattttctcc tagtaatgca tgtatttcac caggataaac cgttaaatta
    52141 accgacttta atgcgtgaac cggaccaaag gacttgccga tccccgccat cgatatatat
    52201 ggcgtggcca taaataacct ctctgcacga aatatcgtat tattgcctga tggctacatt
    52261 tatcaggtct acgttcaggt tattttgttg gccggataag acacttcatt agcatcatca
    52321 tccggcatca ttcagtttta ttgagtgacc aggattgaat cgaccagttt aaattccggt
    52381 gctttatcca gcgggataac cttgccggat ttctcagcgt taaccatcag cttcagaccc
    52441 gttgcgccaa tatctgccgg gttctgcgca accgtcgcgg tcatttgtcc ggcttccacc
    52501 attttgcggg cttccggaat tccatctgta ccgacgacca atattttgcc cgtttttccg
    52561 gcgtttgcga ctgcctgagc aacacccatt gccatcgtgt cattcgcgca atagatcgct
    52621 ttaatattcg gattacgttg caacacgtta gtggcgacat ccagtgcttt aatgcggtcc
    52681 cagtcagcgg gctggctggc gacaagcttg atctggcttg cttttttgaa ggcttcggtg
    52741 gcaccattac gacgcgcttc accggaggcg ttaccggctt taccctcaat gattgcgact
    52801 tcaccacctt cagcgcccaa tttgtcaata atgaacgacg cgcctttcgc cccgacagca
    52861 acgttatcgg tggtgacaaa agcttccaca ttgccgccag cttttttcag attatccatg
    52921 tcgatttttt catcgagatt aaccagataa atgccttttt tccatgcgcg ggcgacaggc
    52981 atgaccagat tcactgagga taatggagcg aaggcgatac ctttgtaatt tttattactg
    53041 agatcttcaa ataactgcaa ttgagattga aaatcgcctt ctgaaggaga ggcaaaaata
    53101 tcaacgctga cgcccagtgt ttttgcttca tcttcaatgc cttttttcat atctacccaa
    53161 aatgggttgg agagggtttt caatacgaca gcatattcgg cggcagcaaa agcgctggtt
    53221 gacaacatta agcccacgag tgtgccgctg aaatatttca gatatttatt cataataatg
    53281 ttctcacggt aaggggacag gtaactccgc gatggcggaa ttaccttttt cttatttcag
    53341 cgcgcctggt gagaagaaat caacaattgc gccggttttt tgcatattaa tattagcttg
    53401 ttcaatgttg agctgggcga cagagacaaa aaaagcgtcc agcaaagtta gttgtaatat
    53461 tcttgccgag gcattacgac ctaataacgg cgtttccggg gctggtgagc aaataatata
    53521 atcggccagt ttcgctatcg gtgaatggta gctatgggtt atacaaataa tctttgcccc
    53581 gttctttttt gccagttcta cggccgcttt tacatcactg gttcgcccgg aatgggtcac
    53641 taccagcaca acatctcctt cctgtaacaa cgaagcggac atcatcatga tgtgagcatc
    53701 aggataggcc tgacagcgta cgccaatgcg caagaacttg tgctgtacat cagcacagat
    53761 agcatttgat cctccggcac cgtacaaatc ccgctgtctg gcctgataga aaaagcgggc
    53821 ggcacggtgg atctcatcaa cgttgacgat cgactgacct tccataatgg tgcgtaaagt
    53881 gatgttaaat accttattca ccacatcctg cggcgcttca tcaaaagcca actcggaagg
    53941 caatacctgt tctgactgag aaaaataatc ttccagcgca ctgcgtaagt tacgaaagcc
    54001 gctaaacccc agcagctttg ataccttaac tatcatcgct tcagataccg acagagcttc
    54061 tgcgacatct ttaattgcgg gtgcacaact caggttaccg ggtttgagta accactctac
    54121 gatgcggctt tcattttctg tcattccttc ctgcttcatt cgcaggtaag gcgctaaccc
    54181 tataccgttc ggaagcgctg aatcaaactc tgactggctc atcacgttct cttgtccttg
    54241 aattgaagac gccactatat caccacagca agaacagaaa agatgctggc gacttcacaa
    54301 aaaaataaaa tttataaagt ttattttatt gtgaacaatg gcgagtggct taaaatttta
    54361 tttcatttaa ttagtttaaa aacatgatgt tatttgtttt tctggttttt tagatgatag
    54421 atgttgctgg atggtgagtg tctttaacgc tattgcgctg tcagatgtaa ctctgtaaaa
    54481 cagatcagga aggcgtaatt catcgatttt gggtgttttt aattcattaa catcacaaat
    54541 gttttttgat tgtgaagttt tgcacggacg gggaagatga atgaaaaaga ttgcatttgg
    54601 ctgtgatcat gtcggtttca ttttaaaaca tgaaatagtg gcacatttag ttgagcgtgg
    54661 cgttgaagtg attgataaag gaacctggtc gtcagagcgt actgattatc cacattacgc
    54721 cagtcaagtc gcactggctg ttgctggcgg agaggttgat ggcgggattt tgatttgtgg
    54781 tactggcgtc ggtatttcga tagcggcgaa caagtttgcc ggaattcgcg cggtcgtctg
    54841 tagcgaacct tattccgcgc aactttcgcg gcagcataac gacaccaacg tgctggcttt
    54901 tggttcacga gtggttggcc tcgaactggc aaaaatgatt gtggatgcgt ggctgggcgc
    54961 acagtacgaa ggcggtcgtc atcaacaacg cgtggaggcg attacggcaa tagagcagtg
    55021 gagaaattga gattcatcca ctacttgcat ggatgagtaa tgactaatgt ggatagagtt
    55081 tctttttgag gttggctaat gaaacgcttt ccgctatttc ttttattcac cctgctcacg
    55141 ctgtccaccg ttccggccca ggccgatatt atcgacgaca ccattggcaa tattcagcag
    55201 gcgattaacg acgcttataa ccctgaccgt gggcgagact atgaagattc gcgcgacgac
    55261 ggctggcagc gtgaagtgag cgacgatcgg cgcagacaat atgacgaccg ccgccgccag
    55321 tttgaagatc gccgcaggca gctggacgat cgccagcgcc agcttgatca ggagcgtcgg
    55381 cagctagaag atgaagagcg gagaatggaa gatgagtatg ggcgatgagg ttgggtatgg
    55441 agcaggcatc gcccctcacc ccaatgggga gagggagaaa accagcgcaa tattcaatat
    55501 cgagcacaat cggtcccctc gcccctctgg ggagagggtt agggtgaggg ggccagcgcg
    55561 cacattccct ctcatcacgc caccccaatc tccatcccat caaaccccgc ctcaaaaccc
    55621 ggcggcagcg cattctccat cagccacgca tcaaactggt ggctgatatg ggtcagaatc
    55681 acccgtggcg agtggataac ctgattgagc gcaagcacgg tatttaaatc acagtgatta
    55741 cgcggcgcat cctcgcgcgg cgggtggctg caatcaatca ccatcacctg cggatgatta
    55801 ttgagtaaaa atttctgtgt tttttccggc aggccagcgg tgtcagatag ccacgccacc
    55861 cggctatgtg ccgtttccag cagataaccg aaggtcagtt ttgagtggtt aagcggcagc
    55921 ggcgtgacct gcaacccctg caaatcaaat accacaaacg gttccactgt gtggctgaaa
    55981 tcaagcaggc ccggatgttt aaacagagcg tcgcaaccct ggtcatccgg cgggccgtaa
    56041 accgggatcg gatcgccaac gccccagcgc agcggaaaca gcccctggac gtgatccata
    56101 tgataatgcg tcagcaaaaa ctgctggaac gatccgggcg accagcgatc ggcgagatcg
    56161 tgcagcccgg cgtcgatcag ggtgattgcg tcgttaaact tcactacgcc gctgcatggc
    56221 tggcggcgat actgcggtga gcgccgcgct cggtcgcagg ccgcgcactc gcagccccat
    56281 gccggaacgc cctgtgcgcc gccggtgccg gtgagcgtga gggtcagact catgttacag
    56341 cgccttggtg aagcggaagt gactctgctc gtagccttct cgcagataga aacggtgcgc
    56401 gtcgtggcgc ttcacgttgg tcgaaagttc ggtcatttcg gccccggcct ggcgggcttc
    56461 ttcttctgcc cacgccagta acttactgcc gacgttcaga ccgcgcgcct gcggcattac
    56521 caccaactcc tgaatttcgc cgatccagtt gacatgatgc agatgaaact gcaaatgcag
    56581 gccgatcata ccgacaactt cgccatccag cagcgccaga tggtagcgca tgttcgggtc
    56641 gcgcaggttg gcgttaaagc ccacgcgaaa cgcgtggtgg tcaaactccg cctgttttag
    56701 ctcgcaaatc aacgcgtaaa ccgcgtcggt gtcgtactgc gtggccgggc gaagctcaca
    56761 agcaggcatg gtgtttctcc ttctgatgga ttagcgtcag cagcgtgtcg accgactggc
    56821 gcaggctgcc gtcattattg agcgtatggc aatcctgtgg agtatagcgg gcggcgcgcg
    56881 ccagccgggc gttaatttaa ctggcgtttt cgcggccacg gttttccagg cgctggcgca
    56941 ggatctctgg cgaaacctgt aagcaaatgg gcagcagcgc cgattgatag cgcgcccgcg
    57001 cctgcggcag atgggcgcgt gagccgttga ccagcacgtc gaatccagcg tgcagccaga
    57061 ggtcaatctc gacgccgacg ccataataca gaccgttagc gtgccagctt aaggccagca
    57121 gattttgccc cgcgcgggta aaaaactcct gctcgctcag ggcgatatgg ttttcgcttc
    57181 cagcgctggc gtcgcgcgtg atgtagcgat gcgccaccag tagctgagtt tgttcccgca
    57241 ggcggagttc cgccagcagg ctatctttcc cggagccgga cggccccatt aaccaaatca
    57301 gttttcccat catcagaaca cccttttacc ctgacgccag acgtggtcga tatgaatgtg
    57361 gttgcccttg cgatgcgcca gcaccaggtc ggcgcgttta ccctcgccaa tcaccccgcg
    57421 atcctggaga ttaagcgctt gcgccggatt tttggtcacc agcttcaccg cctgcggcag
    57481 cgtaaagcgg ttgctctcgt catcggcgac gcggaacgcc gcatccagca ggctggcggg
    57541 gtagtagtcg gaagagagga tatccagcag gccaagctgc gccagttcac tggccgccac
    57601 gttgccggag tgcgaaccgc cacgcacgat atttggcgcg cccatcagca cgttcatgcc
    57661 atgcttgcgc gaggcttccg ctgcttcgaa cgtagtggga aattcggcga tcacgctgcc
    57721 aagctggtgg gattcggcaa cgtgggcgtg cgtggcgtca tcgtggctgg cgagggcaat
    57781 ttgtcttacg cggcacaggg cggcgatagt ttcgcgattc ggctgcgacc agcgtgcggc
    57841 gagcgccagt tgctcttctt cgtactgctg catctgcgca tcggtgagcg agtatttgcc
    57901 ctgataatac tcgcgatact tctcgcggtt ggcgaactgg cgctggcccg gcgagtgatc
    57961 catcagcgac accagcgtca ccggctcgcg ctgcaccagt ttttcaaaca gcggcagcgt
    58021 agtgtgatgc ggcagttcgc agcgcagatg caggcggtgc tcggcgcggt tgacgccgcg
    58081 tttctgcgtc tcttcgatgg cgttgatcat cttctccaga ttttccagcc gatcgccgcc
    58141 gtcgcgcacg tcgccaattg ctacggcatc cagtacggtg gtgatgccgc tcgccaccat
    58201 cagcgcgtcg tggctgctca tcgccgaatg ggcgggccag tcgactttcg ggcgcggggt
    58261 gaagaattta tcgaggttgt cggtatgcag ctcaatcagg ccgggtagta gccagccacc
    58321 ttcgccgtcc atcgcttctg gcaggcggct ctggctttcg gcaaaggcgc ggatttcgcc
    58381 atcctgcacc tccagcgaac cgtgcaccac ctcgttttcc agcaccagct taacgttatt
    58441 gataatcatg aagaggctcc cattgggtgc aggcggtcgg cgacgtcatt acgtacagct
    58501 tcgtcatgga agatgcctac gatggctgcg ccacgggttt tggcttcgcg aatcagttcc
    58561 accaccgcgg cgctgttttt ggcgtccagc gaggcggtag gttcgtcaag cagcagaatg
    58621 gggtagtcga cgataaagcc gcgggcgatg ttgacgcgct gctgttcgcc accggaaaat
    58681 gtcgatggtg ccaggtgcca caggcgttcc ggcacgttca ggcgggtgag aagacgcgcg
    58741 gctttagcgg cgcaggcttc acgcggaacg ccggtatcga gcagcggctg catcaccact
    58801 tccagtgctg agatacgcgg gatgacgcgc agaaactggc tcacccagcc gacggtggtt
    58861 ttgcggattt ccaccacttt gcgcgctggc gcggtgacca ggtctaccca ctcgtcaccg
    58921 tgtttgatct ggatttgacc ttcgtcgggt agatagttgg cgtacagcga gcgtagcaga
    58981 gttgatttgc cgctgccgga atggccgtgg agcaccacgc attcgcccgc gttgacggtg
    59041 agcgaggcgc gattgaggac gggcaggcgc acgccgtttt gctggtgcag gatgaaggtt
    59101 ttactgacgt tttgtacgtt aatcattttt cgcctcgtgg ttcgggtgtc ggatgcgacg
    59161 ctgacgcgtc ttatccgacc tacggggagc gcatttgtag gccggataag gcgtttacgc
    59221 cgcatccggc accggctcaa ttctgcaaaa ccgatgacac cagcagctgt gtatacggat
    59281 gatgcgggtc gtcgagcacg cggtcggtta acccactctc caccacttgc ccctgcttca
    59341 tcaccagcaa acggtccgcc agcaggcggg cgacgcctaa atcatgggtg acaatcacca
    59401 ccgcgaggtt cagctccacc actaggccgc gcagcaggtc gagcaggcgg gcctgcaccg
    59461 acacatccag cccgccggtc ggttcatcca taaacaccag cttcggatgc gtcaccaggt
    59521 tgcgggcaat ctgcaaacgc tgctgcatac cgccggaaaa ggtggtcggc aggtcgtcga
    59581 tccggttggc gggaatctcc acctcttcca gccacttctg cgcggtggcg cgaatgtcgc
    59641 cgtaatgacg cgccccggtc gccatcaacc gctcgccgat attgccgctt gccgagacct
    59701 gacggcgcag gccgtcgagt ggatgctgat gcactacgcc ccattcggtg cgcagcaggc
    59761 gacggcggtc ggcctcgctc attgcataca gcgaacggtt ctcgtagtga atctccccct
    59821 gctgcggcgt cagacgcgcg gagatcgact tcagcagcgt ggtcttcccg gagccggatt
    59881 ctccgacaat gcccagcact tctcccggcc ataaatcaaa agagacatcg ctaaagcctt
    59941 tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag taacggttta ttcattggct
    60001 tttcgcctcg ctctgttggc ggcaataatc ggtatcggag cagacaaaca tgcggtttcc
    60061 ggcgtcatcc agcaccactt catcaagata gctgtgggtc gatccgcaga tggcgcatgg
    60121 ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa tcgagacttt ccacgcgggt
    60181 aaacggcggc accgcataga tgcgcttctc gcgccctgct ccgaacagct gcagggcggg
    60241 catcatgtcc attttcgggt tatcgaattt cgggatcggc gacgggtcca tcacgtagcg
    60301 cccgttcacc ttcaccggat aagcataggt ggtggcgata tgaccgaagc gggcgatatc
    60361 ttcatacagt ttcacctgca tcacgccgta ctcctccagc gcgtgcatag tgcgggtctc
    60421 cgtttcgcgc ggctcgataa agcgcagcgg ttccgggatt ggcacctgga agataatgat
    60481 ctgatcttcg gtcagcggcg tttcggggat gcggtgacgc gtctggataa gcgtcgcatc
    60541 gtccgtacgt tcagtggtgt ttaccccggt cacgcgctta aagaagttgc gaatcgacac
    60601 ggcgttggtg gtgtcatccg caccctggtc aatcaccttc aacacgtcgc tttcgccaat
    60661 cacgctggcg gtgagctgaa tgccgccggt tccccagccg tagggcatcg gcatctcgcg
    60721 cccgccaaac ggcacctgat aaccggggat agccaccgct tttaagatgg cgcggcggat
    60781 catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat tagccatggt
    60841 tctgctcctg ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg acgtagtgcg
    60901 ggagtttgag gtgcgagaca aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga
    60961 actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta ttccggggct tgcaacgcac
    61021 ggtcaaccag cgccattgcc atcgctttgc gctcgctcat gccgaatacc agcccgtagc
    61081 cgcgcgtgaa gtgcggcggc tcatccggcg ggtcgataaa accgttgacc atttcacatt
    61141 cggtcatcag cagttcgccg acgttcaccg caaatcccag ctcttccggc acaatcgaca
    61201 cgtcgatgta accactgcgg atctcgcccg cgaacgggtg attgcgcccg taaccgcgtt
    61261 gggtggagta ggccagcgcc agcaaatagc cttcgtcgcc gcgcatcaac tgctgcaaac
    61321 gggaagaacg tgagcagggg taaaccggcg gcgtgcgggt gatatcatcc ggctgtgcgc
    61381 cgctatcctc ttcaaacttc gccagcccct gacgcgccag caggctgaaa acgtgcggcg
    61441 acggctgttg ttcgctgtcg gcggtggtca gcgtcggcgc ttcaccgttt gccagcaggg
    61501 tgaaatcgag caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg ccgccgggaa
    61561 tgtctttata aaccgccgaa atacgccgtt cgagacgcat ctctgtggtg tcgagcggct
    61621 cgcttaccgc gagcttcgcc agcgtggtgc ggtaggcgcg cagcaggaaa attgcttcga
    61681 cgttatcgcc gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg gcaatgccgc
    61741 cttcggtcat cacgcgatcc accgcaagat taagctgctg ttcaatctgg gcgacgctca
    61801 gttcaggcaa atcggtatcg ccccggcgtc ggctctcttg cagggcgtga gcggcgtcga
    61861 tcgccttctc gccccctttc acggcaacgt acatcagcac acctccacat gagtggttcg
    61921 cggaatagcc agcaggcgct cgccgcaggt caggatcagg tcgatgccga gcgggaacgg
    61981 gtgcgggcgc tcggtgagtt cgtgcagaat gcactccggc agctgcggag cgatcattcg
    62041 ttcttcggca atgcccgcgc cggtgagacg taacatgcgc ccgccgctca ggctggcgac
    62101 ctgtaaaatc agcgttgcac ccgcttccgg cgcaacggcg gtgccggtgg aaagggcgtt
    62161 aagctgttcg ctggaaatcg cctcatccgt caccgcgaag gtcgcctgtt ccggctggct
    62221 gaccagcggc gcgttggtat gaaaacgcag gctctggctg acgatatcgt tactcaatgg
    62281 cgcggcaagc cacaccggcg tgtcgttatc ggccagcgtc agcagcacgc tggtggtggc
    62341 gatattcagc ggctgccagc cgcgcttgag ctgatgcagg gcgacaatca cgcccggctc
    62401 gctcatggcc tttaacaggc gacgaaaact gtgctgggca tcctgcacgg gaagcataaa
    62461 agcggtttcc agggtcatgc gttgtctccg cgaaccatcg taaagaagtc gacccggctg
    62521 gcgttcactt cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca
    62581 ataagggttt ctgataagtt ttgaaaatag cgagattgct gcatcagcgc gtcaatcagc
    62641 gcgcagcgtt cggcgtgctg tttgtcgcgc cccagcaccc agctgtagcc gagcgtgccg
    62701 tcagtcaggc gtacggcggc gcggataacc tcatagtcgg cggtgatgtt cagtgctttc
    62761 aggcgggctg ccagttcagc cggttggctg tgcgccagta cggacatcca gtgctggcgg
    62821 gtcgcggtat ctgcgtgcat tcagtgctcc atagtgaatt caatcatgtc ggcgcgcgtc
    62881 aggctgacgg agtactccgc cgggctgctt tcaccgtcac ggtggttaag ggtgcgcacg
    62941 cacagcagcg gcgacatatt cggaatttca agacgctggc actctttggc ctgggcgcga
    63001 cgggcgctga tccgcgtctg gctgcggcgc agcgcaattc cggtttgctc gcgcagaaaa
    63061 tcgtgcagcg aaccgctgtc gaagcgttgc agcgtcggcc aaagggtgag gtccgcaaag
    63121 tagtggtcga ttaaacagag cgcgacgccg ttgacgcgac gcagggtgcg caaatggata
    63181 acgttctcgc cctcggtaat ccccagcgcg tcagcgacgt gaccggacgc ggggcgcaat
    63241 accgacagca gtttttcgct ggtgggatga ctaccctggt ccagcaggtt ctggctaaaa
    63301 cgcgcctggg cgttgagcgg gtaatcgaac gggcgcatca gtaccagcac gccgacgccc
    63361 tgacggcgct gtacccagcc tttttccacc agttggtcga tggcgcggcg cagggtgtgg
    63421 cggttcacct caaagcgcgc cgccagttgc tgctcggcgg gaagatagtc gccgcagcgg
    63481 tagtgttgac gaagctcctg ctcaagtttt gcggctatct cctgatagcg tgttgggtag
    63541 ctggtcggat gtgtagacaa gtgcatagat atcaatgcct cgcttatcag ataaagtgct
    63601 tacgcaaccg ttgagagagg aaatccagca ggctgaccgt gacgatgata agcaccatca
    63661 gggcgcaggt ttgttggaac tggaaaccgc gaatcgcttc ccacagggtg acgccgatcc
    63721 cgcctgcacc gaccatgccg acgacggtcg ccgagcggac gttggattcg aagcgataga
    63781 gggagtagga gatcagcagc ggcatcacct gcggcagcac gccgtagagg atctcttcga
    63841 gcttgttggc gccggtggcg cgaatgcctt ccaccgggcc gggttcaatc gcttccaccg
    63901 cttcggaaag cagtttggag agcacgccgg tggtgtggat aaacagcgcc agcacgccag
    63961 cgaacgggcc gaggccgacg gcgaccacga acagcatggc gaagaccatt tcgttaatgg
    64021 cgcggcaggc gtccatcagg cggcgaacgg gctggtaaac ccaccacggc accaggtttt
    64081 cggcgctcat caggccgaag gggatggaga gaaccaccgc cagcgccgtg ccccagacgg
    64141 cgatttgcag cgtgacggcc atttcggtga ggtaatcctg ccactggctg aaatcgggcg
    64201 ggaagaagtc ggcggcgaac gtcgccatgt tgccgccgtc tttaatcagc gtgagcgggg
    64261 ccatttccgc gccctgccac gagacgacca acacggcgag aacaacggcc cagctcagaa
    64321 gcgagaacca gctgcgcttg ggtggggcga tggtgatggt ttgcatgttt ggctccggtc
    64381 tgtaggccgg ataaggtgct tgcaccgcat ccggcatcaa cgcctgcaca ttgtcggatg
    64441 cgacgccggg gcgtcttatc cgacctacga tttactgcac cgctttactc accgaactca
    64501 tcgcgcttaa cgcgttgttc aggcggtcca ggtcatccag ctgcgcctga atctcggtgg
    64561 tttttgccag cttgtcctgc tcattcagtc ctttattgct tttcacgccc tgcatctctt
    64621 taaacagcgc gagctggcga atcggcacca gttgcaggtc gctggaagcg cggaacggcg
    64681 cccagcccag gcgttccagc accgcttttt cttccggcgt tttgccgtag ttcataaaga
    64741 agtcgtagat cttgtctttg gtggtttcgg aaaggttttt acgccagacg atcggatcgc
    64801 ctgggatcag cggcgacttc cagatcacct tcagttcttt cagcttctct ggcgcggagg
    64861 ttttcagctt gtcgaggttt tcggtgttgt tggtggcaac atccacctgc ttgttggcga
    64921 cggccagcgc gttggtttca tgcccagcgt tgacggtgcg cttaaagtcg ctggcggaga
    64981 tattgttttt ggcaaagacg tagtaaccgg ggacgaggaa gccagaggtg gagttaggat
    65041 cgccattgcc gaaggtgaga tctttccgct tcgccagcag atcgttcagg ttgttgatcg
    65101 gactatcttt gttgacgatc aacacgctcc agtaacctgg cgatccatcc gccgcgaccg
    65161 tctgggcgaa gacctggccg ttggcgcgat ccaccgcttc cattgccgac aggttgccgt
    65221 accaggcgat atccacttta ttgaagcgca ttccctggat aatgcctgcg tagtctgggg
    65281 caaagaaggc gttcaccttc acgcccagct tcttctccat atcctgtaag aatggcgtcc
    65341 attgcggttt caggttttgc tgtgattccg ttgaaataat gccgaaattc agcgcctttt
    65401 cctgctcttc ggcgtgtgcc gggcttaaca gggtgctgag gctgaacatg ctggtgaagg
    65461 ccagcgaggc aattatctta gcgttcattc gttttcctca atgatgggga tgtcaggcag
    65521 ctttcgcgtt ctcttcgatg cgattaatgc tgcggtagag atggtcaaaa cgttcgttat
    65581 caaactgttg gctgctgccg tcgtagaaaa cgtgcccctg gcgcagggcg acgatgcgtt
    65641 cgcagtagcg cagggcgtaa tccacctgat gcagcgtgac gaccacggtg atgccgtcgt
    65701 tctgattgat gtcgcgcagg gtgtccatca cgatgcgggc ggattccggg tccagcgagg
    65761 cgatgggttc atcggccaga atcaccttcg cctgctgcat cagcgcgcgg gcaatcgcca
    65821 cacgctgctg ctgtccgccg gagagggtgg aaacgcgctg atgggcaaaa tgcaccatgc
    65881 caacgcgggt cagcgcctgt aacgcgcgct gtttctgctc gccggtgaac cagataaaac
    65941 aggtgcgcca gaacggcgtg ctgccgagcg cgccaatcag cacgttctcc agtacgctca
    66001 ggcggttcac caggttgaat tgttggaaga tgtagccggt gttggcgcgg cttttgcgga
    66061 tatcgcgcgc cagacggcct tcgcgctgga ctgtgcggcc cagcagctcg atatggctgc
    66121 cggcggattt atcgccggta atcaaaccgc ttaagtgacg taaaagggtg gatttgccgg
    66181 aacccgacgg cccaagcaga gccaccattt caccgtgatg aatgttcaga tcaaccgcat
    66241 gcagcgcctg atgctgattg aaggttttgg cgagcttctc gacacggata atcgtttgca
    66301 tgatgcagcc tccctaaaaa agtggctcca tcgtggcgga ttattgtgac gatttggtta
    66361 attaaaagtg actaacagat gaagagttaa cgggaattcg atgacagtgc gggagggcgg
    66421 gcctcccggc gggttattgc gttggttgtt gtttgacgac attaatcatc cacggtacgc
    66481 caaatttatc ggtgactttg ccaaagccat gcgcccagaa agtttcctgc caggccattt
    66541 cgatttttcc gttagcggca agattgtcaa accagcgttt tccttcttcg acctgttgcg
    66601 aatcgagcac cagcgtaaag ccggagtagc tggcttttcc tgacggtata gcatcgctca
    66661 tcatgatgtc gcttccggca atgcgcacgt tggcgtgggc gatggcggta tcgggaaatt
    66721 gcattccgga agggcagttc tcggcgctgt cctgcgctga ttttggcatt tcgccgaagc
    66781 tgattttata gagcagttca gcgcccaacg tacgttgata ataagcaatt gcgtcggcac
    66841 agttaccggc aaaagagagg tagggactta acggcatgat ggtgacctca gttaagagaa
    66901 gccagttaag tgtagttcga aatttataca gatgagagag aagcctgata agacgcggtc
    66961 agcgtcgcat caggcagtcg gcatcgttgc cggatgcggc gcgagcgcct tatccggctt
    67021 accgatcaca atacaatcag ttctttttca caaactcaga tttcagtttc atcggaccaa
    67081 aaccgtcgat tttgcaatcg atgttatggt cgccttcaac caggcggatg tttttcactt
    67141 tggtgccaat tttcagcatc gaagagctac ctttcacctt cagatcttta atgatggtaa
    67201 cgctgtcgcc gtcagccagc agattgccgt tagcatcttt aacgatcagc tcgtcgcttt
    67261 cctgtgcagg ttctgcgtcg ttccattcgt aggcacattc cgggcagatg tacatgccgt
    67321 tatcttcgta agtgtattcg gagttgcatt ttgggcagtg tggtaatgac atgtggattt
    67381 cctcaaaagt cagcagggca aaagcgccaa aaaacggcag attgccgaaa aaggccgcaa
    67441 ttatacacaa aatccctgag tttgtcgggg gtactgctga ttttagacga cgatcgtttt
    67501 catttgaaag gaaacgcaaa aagaaaataa tctttttgcc tctatttatt atttaagata
    67561 atgttaatgc tctacttgtt ctttttttat tcacatgata tttttatctt tctgacgttt
    67621 taaatgtatc agaagacgtt atttattctg ataaaaaaca acacactaaa tataaaatta
    67681 tttcttgcag taattataaa agccgaaaac agaactcaaa aacaatctgg ctaaataaaa
    67741 gtaacaaaat ttgctttaag gaagaatttt ctatgtacac acagaccctg tatgagttaa
    67801 gtcaggaggc tgaacgcctg ttacagcttt ctcgccaaca gttgcagtta ctggaaaaaa
    67861 tgcctctctc tgtacccgga gacgacgcgc cacaactggc tttaccctgg agtcagccta
    67921 atatcgccga acgtcacgcg atgctgaata atgagttgcg taaaatttcc cgactggaaa
    67981 tggtgctggc aattgtcggt accatgaaag cagggaaatc aaccaccatt aatgccattg
    68041 ttggtacgga ggttctgcct aatcgtaatc gcccaatgac tgcgctgccg acgcttattc
    68101 gccatacgcc cgggcaaaag gaaccggtac tgcatttttc acatgtcgcg ccaatcgatt
    68161 gtttaattca acaattacaa cagcgcctgc gtgattgcga tattaagcat ctgaccgatg
    68221 tgctggaaat agataaagat atgcgtgcgc ttatgcagcg gatcgaaaat ggcgtcgctt
    68281 tcgaaaaata ttatctgggt gcccagccta tttttcattg tctgaaaagt ttgaatgatt
    68341 tagtgcgact ggcgaaggcg ctggacgtcg attttccttt ttctgcttac gccgccattg
    68401 agcatattcc cgtgattgaa gtggagtttg tccatctggc ggggctggag agttatcccg
    68461 gtcagttgac gttactggat acccccgggc caaatgaagc cgggcaaccg catctgcaaa
    68521 aaatgcttaa ccagcagctg gcacgcgcct cggcggtact ggcggtgctg gattatacgc
    68581 aactgaaatc gatctccgat gaagaggtcc gtgaggcgat tttggcggtg gggcaatcgg
    68641 tgccgctgta tgtgctggtc aataagttcg atcaacagga tcgtaacagt gacgacgccg
    68701 accaggtgcg ggcactgatt tccgggacgc tgatgaaagg ctgtattacg ccacagcaga
    68761 tatttccggt gtcgtcgatg tggggctacc tggcgaatcg ggcgcgccat gagttagcca
    68821 acaacggtaa gttaccagcg ccagagcaac aacgctgggt ggaagatttt gcccatgccg
    68881 cgctcggcag gcgctggcgt catgccgatc tggcggacct cgaacatatt cgtcatgctg
    68941 ccgatcagtt gtgggaagat tcgctgttcg cccagccaat tcaggcgttg cttcatgccg
    69001 cttacgctaa cgcctcgttg tatgctttgc gatctgctgc gcataaactg ttgaattacg
    69061 cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg gttaaacgtc gcttgtgaac
    69121 aattgcggca aaatatccat caggtcgaag aaagtttgca gctattgcaa ctcaatcagg
    69181 cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc cctgacctcc gccaaccact
    69241 ttctgcgtca acaacaagat gcgctgaatg cgcagttagc cgccttgttt caggatgatt
    69301 cggagccgtt aagcgagatg cgtacccgct gtgagacact gttacagacg gcgcagaaca
    69361 ccatcagtcg cgactttacg ctgcgttttg ccgagctgga atccaccctt tgccgggtgt
    69421 taaccgatgt tattcgcccc attgagcaac aagtcaaaat ggaattgagc gagtcagggt
    69481 ttcgtcctgg gtttcatttt cctgtttttc acggcgtagt tccccacttc aacactcgcc
    69541 agctgttcag tgcagtcatt tcgcgccagg atgcaacgga cgagcagagc acgcgtttag
    69601 gcgttgtgcg tgagactttt tcgcgctggt tgaatcagcc cgactgggga cggggaaatg
    69661 agaaatcccc gacagaaacg gttgattaca gtgtgttgca acgagcatta agcgcagaag
    69721 tcgatcttta ttgccaacaa atggctaaag ttctggcaga gcaggtcgat gaatctgtta
    69781 cggcaggcat gaatactttt ttcgctgagt tcgcttcatg tttgacggaa ttacagacgc
    69841 gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc ggtggtcagg ctgatgcagc
    69901 agcaattgca gcagactgtg atgactcacg gctggattta caccgacgcc cagctgttac
    69961 gcgatgatat tcaaacactt ttcacggcag aacgatattg accaagacgt tactggacgg
    70021 ccccggtcgc gtgctggagt cggtttatcc ccgcttttta gtggatctgg cgcagggcga
    70081 tgatgcccgc cttccacaag cccatcagca gcagtttcgt gaacgactga tgcaggaact
    70141 tctttcgcgt gtgcagcttc agacatggac gaacggcggc atgttaaatg cgccgcttag
    70201 cctgcgactg acaatggtgg aaaaactggc gtcgatgctg gatcccggtc atctggcgct
    70261 gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg gatcaccgca agcactctgc
    70321 tttcccggag ctcccccagc aaattgccgc cttgtatgag tggttttcag cccgttgtcg
    70381 ctggaaggaa aaggcattaa cgcaacgagg cctactggtg caggcgggtg aacagagcga
    70441 gcaaattttt acccgctggc gtgctggggc gtataacgcc tggtcgttgc ctgggcgctg
    70501 ttttatcgtt ctcgaagagt tgcgttgggg ggtatttggc gatgcctgtc gtctgggaag
    70561 cccgcaagcg gtggcgttgt tgctgggtga tttgcgcgtg aaagcgacac aacatctggc
    70621 agagagtatc aatgcggccc cgaccacgcg tcactattac catcagtggt ttgcctcttc
    70681 gaccgttccg acgggcgggg atcatgctga ttttttaagt tggctgggaa agtggaccac
    70741 ggcagataaa caacccgttt gctggtcagt gacccaacgc tggcaaactg tcgcgctggg
    70801 gatgccacga ctctgttcag cgcagcgtct ggcgggggca atggtcgagg aaatcttctc
    70861 tgtaaatttg gcgtaaataa tcagttacat caatgagtcc taaacgaaat ccatgtgtga
    70921 agttgatcac aaatttaaac actggtaggg taaaaaggtc attaactgcc caattcaggc
    70981 gtcaactggt ttgattgtac attccttaac cggagggtgt aagcaaaccc gctacgcttg
    71041 ttacagagat tgcatcctgc aattcccgct ccccttttgc ggccgtcgcg ctgatttttc
    71101 tggcgtttgc ggaaatgggc caactctgcg aggaaagcta tgctgaaaag gaaaaaagta
    71161 aaaccgatta cccttcgtga tgtcaccatt attgatgacg gtaaactgcg taaagccatt
    71221 accgcagcat cactgggtaa tgcaatggaa tggtttgatt ttggtgttta tggttttgtt
    71281 gcttacgcat taggtaaagt ttttttcccg ggggctgacc ccagcgtgca gatggttgct
    71341 gcacttgcca cgttctccgt tccctttctg attcgaccgc ttggcgggct cttctttggt
    71401 atgttgggcg ataaatatgg tcgccagaag atcctcgcta tcactattgt gattatgtcg
    71461 atcagtacgt tctgtattgg cttaataccg tcctacgaca cgattggtat ttgggcaccg
    71521 attctgctgt tgatctgtaa gatggcacaa ggtttctcgg tcggcggtga atataccggg
    71581 gcgtcgatat ttgttgcgga atactcacct gaccgtaaac gtggctttat gggcagctgg
    71641 ctggacttcg gttctattgc cgggtttgtg ctgggtgctg gcgtagtggt gttaatttcg
    71701 accattgtcg gcgaagcgaa cttcctcgac tggggctggc gtattccgtt ctttattgct
    71761 ctgccgttag ggattatcgg gctttacctg cgccatgcgc tggaagaaac tccggcgttc
    71821 cagcagcatg tcgataaact ggaacagggc gaccgcgaag gtttgcagga tggcccgaaa
    71881 gtctcgttta aagagattgc caccaaacac tggcgcagcc tgttgacatg tattggtctg
    71941 gtaattgcca ccaacgtgac ttactacatg ttgctgacct atatgccgag ttatttgtcg
    72001 cataacctgc attactccga agaccacggg gtgctgatta ttatcgccat tatgatcggt
    72061 atgctgtttg tccagccggt gatgggcttg ctgagtgacc gttttggccg tcgtccgttt
    72121 gtgctacttg gtagtgttgc cctgtttgtg ttggcgatcc cggcgtttat tctgattaac
    72181 agtaacgtca tcggcctgat ttttgccggg ttactgatgc tggcggtgat ccttaactgc
    72241 tttacgggcg ttatggcttc taccttgcca gcgatgttcc cgacgcatat ccgttacagc
    72301 gcgctggcgg cggcatttaa tatttcggtg ctggttgccg gtctgacgcc aacactggcg
    72361 gcctggctgg tcgaaagctc gcagaatctg atgatgcctg cctattacct gatggtagtg
    72421 gcggtgattg gtttaatcac cggcgtaacc atgaaagaga cggcaaatcg tccgttgaaa
    72481 ggtgcgacac cggcggcgtc agatatacag gaagcgaagg aaattctcgt cgagcattac
    72541 gataatatcg agcagaaaat cgataatatt gaccacgaga ttgccgattt gcaggcgaaa
    72601 cgtacccgcc tggtgcagca acatccgcga attgatgaat aagctgaaac ggatggcctg
    72661 atgtgacgct gtcttatcag gccaattgaa ctcttaaggt tcacttaatc tctgacgcgc
    72721 atactctcct ccaggttaac ggaggagagt gcaatgaaaa accgtgttta tgaaagttta
    72781 actaccgtgt tcagcgtgct ggtggtcagc agctttcttt atatctggtt tgccacgttc
    72841 tgatctttct tcagccgtac ccaggcccgc gtgccggaag tctcttgccg gttttgcagg
    72901 aaaaactgcc cgtgatgcaa ctgtgtaatg cggctgacaa tacttaaccc cagaccaatc
    72961 ccgccataac ggctgtccat acgtacaaac gctttactca actccccgca tttactctca
    73021 tcaatacctg gtccttcatc ttcaactgcc atgaccgctc cgtcatcttc ttgcagctta
    73081 atcataatgt tgctgccttg cgggctgtaa cgatgggcgt tttctaccag gtttcgcaat
    73141 aacatccgca gcagggttgc atcaccctga atggtgatgt cggcggcgct ctctggcaat
    73201 agcagggttt gctgtcgctg gtcgagcatg gtactgagtt cgtcatacga ggggagaatg
    73261 acatcttcca gcagttttac atgttgataa ttaccggaag aaaatgactg tccggcacgc
    73321 gccagttgca gcagctggga gacgctctcc atcatctgat caagtcgtgc cactaacggt
    73381 gctacatcaa tgtgatgcgt tttcgccagc agttccagat gcaaacgcac ccccgccagt
    73441 ggcgttcgca gttcgtgcgc gacgtcagcg gtaaacaacc tttcgttatc cagcgtgctg
    73501 gtcaggcgac tgaccagatc gtttaacgcc gaaaccaccg cttcgatttc gagggtggcg
    73561 ctgtgaatgg caatgggcgt taagttgtcg gcagtgcgcg cttccagctc tttttgcagc
    73621 tccgccagcg ggcgggtgat gcggcgcacc gcctgatagc agataaatag cgtcaggctg
    73681 accataaaga cgccggggac aatcaggctg gcgaccgcct cgcggatctc acgcatgatg
    73741 tggcgatcgt tgttgcgatt gtcgcgtagc gcctgctcaa acagctgaat ctgctcggta
    73801 ctttcatgcc atagccagaa gacgctgatc agctcaaaca ccaacaaaat ggccccgatg
    73861 gtcaatatca gccgttggcg cagcgatatt ggtcggcgca gaaaatgcat cagattcaat
    73921 tagttttcct cattcgcgac cagcatatag ccaaagccgc gcacggtgcg gatacgggct
    73981 ttgcccactt tgtcgcgcag attgtggata tgcacttcca gggtgttggt cgagggttca
    74041 ttgtcccagt tatagatgtc gttgtagaga atttcccgat gcaccggact gcctgctttg
    74101 agcattaacc gtgacagcag agcatattct ttgggcgtca gaatcaactc ttcaccgccc
    74161 atccatacct gacggcgacc catattcagc gtcagattgc caacaatcag ctcactttcg
    74221 ccctgattat tatggcgtcg tagcagggcg cggatacggg cgtgtaactc ttccagcgca
    74281 aaaggcttca ccagatagtc atcggcaccg acatccagcc cagcgatttt gtcggtcagc
    74341 gtatcgcgag cggtgaggat cagtactggc agggtgtatt ttttctgccg gatacgggcg
    74401 agaaaatgca gtccatcttc gtcgggtaac cctaaatcca gtaccaccag gctgtaatga
    74461 ccggcctcaa ggctttgttc cgccatccgc gcggttgtca cgccatcgca cgcgtagcct
    74521 tcagtttgcg ccgccagaat caatccctgc aataacagcg tatcgtcttc aacaatcaga
    74581 attttcattc actcactctc ctgcaagttt gcagaatatc atccgcagcc tggtaatact
    74641 tcgtctcaac gccagttaat cccaatagcg tggagaataa attgtcttgt gaatagtgtt
    74701 gcgtttgcgc ctgtttttgc aggcagttct ggtcaacctg ataccgtttt tgataatcct
    74761 ccgacagcca cagcagcatc ggcacctgtt tttggctatc cggggcgatg gcataaggca
    74821 gaccgtgcag atagatgcca ttttcaccta acgattcacc gtggtcagaa agataaacca
    74881 ggctggtggt aaatttatcc tgatgttctt tcagcagatt aatcgcttta tcaacaatat
    74941 agtcgacgta aaccagcgtg ttgtcgtaag tgttcaccag ttgctctttg ctacaggtct
    75001 ggatctcatt ggtgtcgcag gttggggtaa atttcctgaa ctgcggcgga tagcggttgt
    75061 aataggtcgg accgtggctg ccgatggtgt gtaagacaat cacgccatca ccctgcaggt
    75121 tattgatgta ctcttccagc ccgtggaaca gcacttcgtc atagcattcg ccgttgatgc
    75181 actgaccagg cagattcagc gcggtgacgt tctggtgagg cacgcggtcg caggcacctt
    75241 tacagccgcc atcgttgtca ttccacagca cgttgatgcc cgctcgctga atgatatcca
    75301 gcacgccttc ctggtgctgt gccagctctt ctttgtaatg ctcacgcggc atatccgaga
    75361 acatgcacgg tactgaaact gccgttgccg tgccgcaaga tgcggtatta gggaaataga
    75421 ccacgttatc tttcgccagc cgcgggttag tttcacgcgg gtagccgttg agggagaagt
    75481 tctccgcccg cgaggtttcg ccgacaatca ggatggtcaa atttttacgt ttttcgttct
    75541 gcattaacgg gttgcggtgg gcgtcttcac caattcgcac cagcggtaga tttgccagtc
    75601 gctgatggga gtaccatgac cagctggcaa caatgctgtt agaggggctt aaggatttca
    75661 ccagctcttt gttattgcgg aacaacgagg cgtagtcttt ataaaacagt gcggcgacca
    75721 gcaaaatcag tagtacagaa accagaatat tggctccacg gaaaagaaca ctgcgcagac
    75781 gcgaggtggc aggtttgatt tttatccagc aggcaatcag cgcagcaagc acgccgctga
    75841 atcccagcgt taataacatt tgcggtgtca tcagcgcata actttctgcc ggagtggtat
    75901 caataatatt ggcaatcatc gagcggtcga tgacgatgcc gtaagtcatt atgaaatatt
    75961 gtgcagccgc gccaaccaga ataaacaggc aggccagtgg tcgattaagc cataagaaag
    76021 agcttagtgt caggacaata ttaatcacgc tgaaagcgac gaccggcatc gacaagaaaa
    76081 ccagtacgtt atgcagcgaa tccagcggca gcgcctgcaa cacctgttta aaaaaggcaa
    76141 tattcaggca gatagagata taaaaagcgg ccaacaatag ccaggcgagt aaattcaaag
    76201 agggtctttt tagtaggcgc ttcaacatca cggtgtttcc atcgaacaaa gtgcgcatat
    76261 gctcgcaaag caaaattaag ccaaccttaa gttcttaagg ttggctttta tgtttgctgg
    76321 attaagggga attaatcttt gcttattggt gcatctaagg gatacgggtt tttatgtagc
    76381 cggttgtaat tcagcgcata catggcggtg atgaccatca gggtgacaaa tgaccacata
    76441 acctctttcg ctccggaccc caccacggcc cagatgcagt agaggaaggc aatggtagta
    76501 actgccagat atgccgggcg tgctttacca aagtgaccgt gtccgagcag cagtaacgcc
    76561 gcacaggtgt aaagatatgg caccagtgta aagatgaccg agacggaaga aaccagaccg
    76621 aactctttgg tcgcgtttgg tgaaatgctg ctgagctgga atatggtcat caaaataccg
    76681 acgataatca gccccgccac tggcgtaccc gctttattta cacgggcaaa aatcggtggg
    76741 aacagtccgt catcggcagc ggctttcgcc gtttgacccg ccagcaacgt ccagccgccc
    76801 agtgaaccta agcaacccgc agctgcgcag aaggaaacaa tggccccggc ggtgtcaccc
    76861 aacgccatcc gtgcggcatc accgaatggc gaagcagaaa cgcgcagtgc ggcattaggg
    76921 atcatcccca taatcgcggt ggtagaaagt acatagcaaa cggcggcaat caatacccca
    76981 ccaatggtgg cgatagggac attgcgtttc gggtttttca ccacacctgc ggcaacggag
    77041 gcactttcca caccgatgaa cgaccacagc gtaacgttaa gggtactttg aattgcaccg
    77101 aaggtgccca ggccgctgac gttccatgcc gccatatagg tttcaccacg gaaccagaac
    77161 cagccaaata cggcaatccc gacgatgggg atcagcgcca gcacggtggc aactgcctgc
    77221 acacgggtga tcattttcgg accgacaatg ttcagcagga cgaagatcca cagcaccacg
    77281 acgcaggtga tggttaatac caatggatct ttcagaatcg ggaagaagta acttaaatat
    77341 cctacgccaa tgaccaccat ggcgatattg ccgatccagc aggccagcca gtagaggacg
    77401 ttggtttgat aaccgagaaa cgggccaaag cagcggcggg cgtaagcgta agaaccacca
    77461 ggacttggat cgaggaacga cattttggcg tataccatcg agagccccag cgcaccgata
    77521 atcgtcacca accatccata aatggcaatc ccgccagtcg aggccaggtt tgcaggtaac
    77581 agaaaaacac ctgaccccat aatattcccc gacaccatca gggtgacggg gattaagccc
    77641 actttgtgag catcagcatc cgaagacata attaaactcc tgcgaaggcg agcttcgtga
    77701 caataaatta cgtcatatca tacgcctgca ttcgatagtt gaattattat cgaccgggtt
    77761 atcgctgatg ccgttattaa tatagctctt cgttaaggtt aaaaatcagg cgacagtgcg
    77821 ttctctgtgc tgactaacat aatgcagcgg cgtcataccg tagaagtctt taaagacaga
    77881 aataaagtac gacgtactgt tgtagccgca ggactgtgat acctgagaga tatttttacc
    77941 gtccatcatt aattcattta cggcataacg catgcggcag gtggtgatta tttggctata
    78001 actggtgttt tcgcttttca actttttctt taacagactt gggctaagac ataaacaact
    78061 ggctaccata ctaagattcc agtctttgtg aatatcgctt tcaataattt gataaacgct
    78121 gtcacttaca caattacgta acatatacat cattaatgaa aggaattttt tactgtcgag
    78181 aaaacgggat agcaccgtga ataataatgc gcgtgttcgt tccttttctg actcagtttc
    78241 tgcgggcatc atgctgtgtt gcgcggcaac gcgaaagacg tctggcgtca ggcatggcag
    78301 ggtgagtatg ggcgtagcgc tacgttgcca tactggtatt tgcgagagat ctttattcag
    78361 aaaccggagg taatctttga tgatgtcgtg actaatatga gcaaccaaag tgttgttaag
    78421 agaggagacg tcgataatat tattttcaca atttaataac gccatatggt tagctttaag
    78481 gctaataggt tctttcccat tcacccttat ccagacatct ttttcagtca ataaaacaat
    78541 acaaggttgg tcgctgcaaa tcctcataca tgtactcctg agtgcgaata aaagaccagt
    78601 tctcgctaaa gcaaagcgat accgggaaat ggttatataa ttgtatgatg aatataaaca
    78661 tgcataaaaa taacgagttt tactaatatt acaaattttt atagtaattt ctcatgtaca
    78721 tattttttta caggataaat aacaggcaaa aaaaagcgcg gggtggggcc gcgcaaaaag
    78781 tatgacattt ttgtttgcag caatttttat gaaaacgttg ccatactcaa aaggcctaag
    78841 gccaggcggc gtaatgttat ttaaacaatt acgccttcag cggaatagtg gttacgcttt
    78901 cacgcacata acgtggtaaa taccgtcaat aatttcagtc ccttcagttt cgtgttcaaa
    78961 tccagggaaa tggtggtccc aggattgcag cgagcgtaaa taacttactt gcggactgtt
    79021 tttatcgccg aagttttcac cagacagcag catcgggatt cctggcggat acgggataac
    79081 tgagtttgcc gcgatgcgtc ctggcagatt ttcaatggat accagttcga cattgttgtc
    79141 gacaatcgcg ttgtacgctt cacgcggggt gacttccgcc accggcaggc cggaataggc
    79201 ttcgttcaac cgtgcgccag ggttgttttc tttcagccag gcaaacatgg tgtcacccag
    79261 atcgtgaatc cccatgttcg cgtaagtgtc aggatattgt tcaacaagtt ccggcatcac
    79321 ctgcgccagc ggtgtgttgg cgtcatagtg gcgtttgaag gagcaaaggg tgttaaccag
    79381 agttccccat ttcccacggg ttacgcccat agagaacagg aacataattt ggaagtcagt
    79441 ggtgcgggta ggtacaatgc cgtggcgacc aagccaggca gtgaccagcg ccgccggaac
    79501 accggtttct tccagttcac catcttcacc cattcccgga gcaaggatgc tgactttaat
    79561 cgggtcgagc atactccagt tatccggaat atctttgaag ccgtgccagc tttcgcccgg
    79621 atgcattacc cagcagtcct gaacggtggt cagcagtttg gttggtgcgt cagcaaagtc
    79681 ataggttttg ccggtttgtg ggtcggtgac gacttctttg ttccacggtt tgaagaacca
    79741 gctaccgtca gcggtgaact ctttatatag ccgcgccatc gcctgacgga aatcaaccgc
    79801 ttcgtcaatc acttcctgtg tcagtgacag gccgctgttg ccgtccatca tcgacaccgc
    79861 cacgtcgttg gatgcgcaga tggcatacag cggggaggtg gtggcatgca tcatgtaggc
    79921 ctggttgaag cgggagaagt taatcgcccc acgaccttca cgtacatgaa tataagaagc
    79981 ctgtgacagc gcattcagca gtttgtgggt ggagtgggtg gcgaaaacgg taggaccgtt
    80041 gtgatcgcca ggttcgccgc gcatggcata gtgatcggca tagatcgggt tgaaacgtgc
    80101 atagccgtac caggcttcgt caaagtgcag acgatcggag gttttttcca gcagatcctg
    80161 cgcttcttta gcgttataac acacgccgtc ataggtgcag ttggtcacca cgcagtaaga
    80221 cggtttttgc ccggctttgt ctttggtcag cgggctttca ctgattttct tctgcaaggt
    80281 ttcaggttgc atttcctgcg gatagattgg cccgataatg ccgtagcggt tgcggcttgg
    80341 caccatatag accggtttcg cgcctgtcag catcaaacct tgttcgatgg atttatggca
    80401 gttacggtca acgaccacga catcgttatc tgtcatgcaa gcctgcatga tggtgcggtt
    80461 agagccggaa gtaccgacga ctaccgacca ggagcgatcg gcaccaaata cgcgtgcggc
    80521 atatttttcg ctttcgccaa atgcgccagt atggtcaagc aaagaaccga gggaagttcg
    80581 ttcgatgccc atgtcggtgc ggaacagatt ttcaccatag tagtcatggt agaaacgtcc
    80641 ggcgggtgtt ttggtaaaac caacgccgcc ctggtggcct ggcgctgccc aggaatattc
    80701 atggatgtca ctatatttca tcagcgcgct gaacagtggc ggcaacagct gctggcggta
    80761 gcgggtcatc gcggcaacgg cgcgtccggc gataaagtcg gcggtatctt ccagaatcca
    80821 ggcgaattca tcgacaagct ccagcaggtc gcgatccatt gcggcaaggg ctttttcccg
    80881 atcgcccaac aggaagaccg gcacgttttg ttggcgctca tgaagcttac cgatcaattg
    80941 tctgacgttt tgatgttcgt ccggatgttc catttgatag ctgaacatca ggcagtcaat
    81001 ggcttcgttt gaagagagaa tggcaaaacc atcatcaaag gaggtggatt taatcacggt
    81061 aacattttgc tggcttaaag catctgccag acgctcaacg gcgttaccga cccaggtgtc
    81121 ttgatggaga aactcgcttt caacaattaa tactttcatc attgcttacc cggttatgaa
    81181 ggaatcttcg ttgcgggcaa gtatcttccg gccttttcgg gctgtaaagc gcgtgaaaaa
    81241 caaacatttt tgaaatattt atttttataa acaatgcgtt atgtgattat tttaaattat
    81301 tttcactgct tattatcgac ggctaaacta tttttttggc tgatactgat atcgtcttta
    81361 gccgggaaac gtctggcggc gctgttggct aagtttgcgg tattgttgcg gcgacatgcc
    81421 gacatatttg ccgaacgtgc tgtaaaaacg actacttgaa cgaaagcctg ccgtcagggc
    81481 aatatcgaga atacttttat cggtatcgct cagtaacgcg cgaacgtggt tgatgcgcat
    81541 cgcggtaatg tactgtttca tcgtcaattg catgacccgc tggaatatcc ccattgcata
    81601 gttggcgtta agtttgacgt gctcagccac atcgttgatg gtcagcgcct gatcatagtt
    81661 ttcggcaata aagcccagca tctggctaac ataaaattgc gcatggcgcg agacgctgtt
    81721 tttgtgtgtg cgcgaggttt tattgaccag aatcggttcc cagccagaga ggctaaatcg
    81781 cttgagcatc aggccaattt catcaatggc gagctggcga atttgctcgt tcggactgtt
    81841 taattcctgc tgccagcggc gcacttcaaa cgggctaagt tgctgtgtgg ccagtgattt
    81901 gatcaccatg ccgtgagtga cgtggttaat caggtcttta tccagcggcc aggagagaaa
    81961 cagatgcatc ggcagattaa aaatcgccat gctctgacag gttccggtat ctgttagttg
    82021 gtgcggtgta caggcccaga acagcgtgat atgaccctga ttgatattca ctttttcatt
    82081 gttgatcagg tattccacat cgccatcgaa aggcacattc acttcgacct gaccatgcca
    82141 gtggctggtg ggcatgatat gcggtgcgcg aaactcaatc tccatccgct ggtattccga
    82201 atacagcgac agcgggctgc gggtctgttt ttcgtcgctg ctgcacataa acgtatctgt
    82261 attcatggat ggctctcttt cctggaatat cagaattatg gcaggagtga gggaggatga
    82321 ctgcgagtgg gagcacggtt ttcaccctct tcccagagag gcgaggggag cgtccgagca
    82381 tcatgaggcc gaaaactctg cttttcaggt aacttattac cataaactca gatttactgc
    82441 tgcttcacgc aggatctgag tttatgggaa tgctcaacct ggaagccgga ggttttctgc
    82501 agattcgcct gccatgatga agttattcaa gcaagccagg agatctgcat gatgactgca
    82561 cccaaaatta catttatcgg cgctggttcg acgattttcg ttaaaaatat tcttggtgat
    82621 gtgttccatc gcgaggcgct gaaaacggcg catattgccc tgatggacat tgatcccacc
    82681 cgcctggaag agtcgcatat tgtggtgcgt aagctgatgg attcagcagg ggccagcggc
    82741 aaaatcacct gccacaccca acagaaagaa gccttacagg atgccgattt tgtcgtggtg
    82801 gcatttcaga ttggcggtta tgaaccttgc acggtgactg atttcgaggt ctgtaagcgg
    82861 catggtctgg aacaaaccat tgccgatacg ttggggccgg gcggtattat gcgcgcgcta
    82921 cgtaccattc cgcatctgtg gcaaatttgc gaggacatga cggaagtctg ccccgatgcc
    82981 accatgctca actatgttaa cccaatggcg atgaatacct gggcgatgta tgcccgctat
    83041 ccgcatatca aacaggtcgg gctgtgccat tcggtgcagg gaacggcgga agagctggcg
    83101 cgtgacctca atatcgaccc agctacgctg cgttaccgtt gcgcaggtat caaccatatg
    83161 gcgttttacc tggagctgga gcgcaaaacc gccgacggca gttatgtgaa tctctacccg
    83221 gaactgctgg cggcttatga agcagggcag gcaccgaagc cgaatattca tggcaatact
    83281 cgctgccaga atattgtgcg ctacgaaatg ttcaaaaagc tgggctactt cgtcacggaa
    83341 tcgtcagaac attttgctga gtacacaccg tggtttatta agccaggtcg tgaggatttg
    83401 attgagcgtt ataaagtacc gctggatgag tacccgaaac gctgcgtcga gcagctggcg
    83461 aactggcata aagagctgga ggagtataaa aacgcctccc ggattgatat taaaccgtca
    83521 cgggaatatg ccagcacaat catgaacgct atctggactg gcgagccgag tgtgatttac
    83581 ggcaacgtcc gtaacgatgg tttgattgat aacctgccac aaggatgttg cgtggaagta
    83641 gcctgtctgg ttgatgctaa tggcattcag ccgaccaaag tcggtacgct accttcgcat
    83701 ctggccgccc tgatgcaaac caacatcaac gtacagacgc tgctgaccga agctattctt
    83761 acggaaaatc gcgaccgtgt ttaccacgcc gcgatgatgg acccgcatac tgctgccgtg
    83821 ctgggcattg atgaaatata tgctcttgtt gacgacctga ttgccgccca cggcgactgg
    83881 ctgccaggct ggttgcaccg ttaaaacgcg actaaacgct actgcgccgg gggatttatt
    83941 ccggcgcaca cctctgacga taccaataac agaaggcggg cgttggtaac agcgacccga
    84001 taccctatga gcatttcaat gactacaaaa ctcagttatg gatttggagc gttcgggaag
    84061 gattttgcga tcggcattgt gtatatgtac ctcatgtatt actacaccga tgtcgttggg
    84121 ctgtctgtgg gtttggtcgg tactttgttt ctggtggcga ggatctggga tgctattaac
    84181 gatccgatta tgggatggat tgtaaatgct acgcgatcgc gatggggtaa gttcaaaccc
    84241 tggatcctga tcggtacgtt ggcaaactct gtaatcttat ttctcctctt tagtgcgcat
    84301 ctgtttgaag gtactactca gattgtcttt gtttgcgtga cctacatcct ctggggcatg
    84361 acttacacca ttatggatat tcccttctgg tcgctggttc caaccatcac gctcgataaa
    84421 cgtgagcgcg aacaactggt tccttatccg cgtttttttg ccagtctggc aggctttgtt
    84481 acggcaggtg tgacgctacc atttgttaat tatgtcggcg gtggcgatcg gggatttggc
    84541 tttcagatgt tcactctggt actaatcgcc ttttttattg tttcaaccat catcactctg
    84601 cgcaatgtgc atgaagtctt ttcgtcagac aatcaaccgt ctgctgaagg aagccatctg
    84661 acacttaaag ccatcgttgc gctaatttat aaaaacgatc agctttcatg cctcttgggt
    84721 atggctcttg cttataatgt agccagcaac attattaccg gatttgctat ctattatttc
    84781 tcatatgtta tcggtgatgc ggatttgttc ccctattatc tgtcgtatgc gggagctgct
    84841 aacctggtga cgttagtatt cttcccacgc ttagttaaat cattatcccg acgcatttta
    84901 tgggccggag catctattct tccggtgtta agctgtggtg ttctcctgtt aatggcatta
    84961 atgagctatc acaacgtcgt cctcattgtg attgcgggta ttttgctgaa tgtgggaacg
    85021 gcgcttttct gggtattaca ggtcatcatg gtggcagata ccgttgatta cggtgaatat
    85081 aaactgcacg tacgctgtga aagtatcgct tactccgtgc agactatggt ggtgaagggc
    85141 ggttcagcct ttgcggcttt tttcattgcg gttgtgttag ggatgattgg ctatgtaccg
    85201 aatgttgaac agtctacgca agccctatta ggtatgcagt ttattatgat tgctctacca
    85261 actctgtttt tcatggtaac gctgattctc tacttccgtt tctatcgcct caatggcgac
    85321 acgctgcgca ggatccagat tcatctgctg gataaatatc gcaaagtacc gcccgagcct
    85381 gttcatgctg atattccggt cggtgcagtg agtgatgtga aagcctgacg tggacaatag
    85441 ctaacgaaaa tggcctgatg cgatatgttt atcaggccac gtcttaatgt aatgctttgg
    85501 atttgcaaat tttgtagacc ggataaggaa ttcacgccgc atccggcatc aacaaagcgc
    85561 acgttgttat ccggttatca agccaaagcg ccgtagctgg cggcaatgaa agcgagcgag
    85621 gaatatcacc gtcgttaatg cgccaagcag cgcgcagaac atatcggatt gcgtgtccca
    85681 ctggtcgccc tgggtgccga gaaaatcatc cgctccctgg cccattgcca gcgccgccca
    85741 ccactcgatt aattcataca tcgcgcttat cgccagcgcc acgcagcaga ccaggaacgc
    85801 caccatttta cgtccgcgca cgtacatccc gcgcacgaga atttctcgtg ccaccagtgc
    85861 aggcaccagc ccctggaaaa aatgccccag cttgtcatac ggattacggc tcaaccccag
    85921 ccattcctgc acctcaaaac cgatggggac tttcgcgtag gtgtattgcc cgccgaccat
    85981 caggatgatg gcgtgaaaga aaatgagcgt atagagcagc ggcgttaacg gataacgttt
    86041 agcggtggca agcagtagcg gcacgacaat aatcaccggt gtcacttcca tcagccaggt
    86101 gagtttgtca tgggccgaaa tgccggtata aatcaggatt aacgttagcg ccagtgcgcc
    86161 ggtgttaaga attaacggct tgagtgtgcg ggtcatggtg agttcgcaag tcagggaaaa
    86221 tactgactat tcaccagcaa gcgtgaaatt acaatcgcaa aagaggtggc ccagggggat
    86281 cacctggcag catgctgcca ggcgctgggc cgaagaggtt acttagtgca gttcgcgcac
    86341 tgtttgttga cgatctgctg gaagaagtcg ttacctttgt catcgaccag gataaacgcc
    86401 gggaaatctt ctacttcgat tttccagata gcttccatac ccagctccgg ataagcgacg
    86461 cattccagat gcttgatgct ctgctgcgcc agtaccgccg ccggaccgcc gatgctaccg
    86521 aggtagaagc cgccgtgttt atgacacgcg tcggtaacct gctgactgcg gttacctttc
    86581 gccagcatga tcatgctgcc gccgtgggat tgcagcagat ccacgtagga gtccatacgg
    86641 cctgcggtgg ttgggccaag tgaacctgat ggataaccgg caggggtttt cgccggaccc
    86701 gcgtagtaga tcgggtgatc tttgatgtac tgcggaagtt ctttaccggc gtcaatcagc
    86761 tctttcagct tggcgtgtgc aatatctcgg cccacgataa tggtgccggt gagcgacaaa
    86821 cgagtggata ccgggtattg cgaaagctgg gcgaggatct ctttcatcgg gcggttaagg
    86881 tcaactttca ccgcttcgcc ttcaccggcc tggcgcagtt cttgtggaat gtactggcct
    86941 gggttgtgtt ccagtttttc gatccagata ccttcgcggt tgattttcgc tttaatgtta
    87001 cggtcagcgg agcaggagac gcccatgccg accgggcagg atgcgccgtg acgtggcaga
    87061 cggataacgc gaatgtcgtg cgcgaagtat ttaccgccaa actgcgcgcc aagaccgagt
    87121 ttctgggcct cttccagcag ttcctgttcc agctggacat cgcggaacgc ctgaccatgt
    87181 tcgttccctt ccgtcggcag ttcatcgtaa tagtgagcgc ttgctaactt gacggttttc
    87241 aggttggttt ccgcagacgt accgccaatc acaaacgcga tatggtacgg cgggcaggct
    87301 gcagtaccga gggtacgcat tttctcgacg aggaagtttt tcagtttgcc gggagtcagc
    87361 agggctttgg tttcctggta gagatacgtt ttgttggcag agccgccgcc tttcgcaacg
    87421 caaaggaatt tgtactcatc gccatctacc gcgtacaggt cgatttgcgc aggcaggtta
    87481 gtgccggtgt tgacctcttt gtacatgtcc agcgccgcat tctgtgaata gcgcaggtta
    87541 tcttcgatat aggtgttata gacgcctttc gacagcgctt cttcatcacc gccgccggtc
    87601 cacacgcgct ggcctttttt accgacgatg atcgcggtgc cggtatcctg gcaggtcggc
    87661 agcacgcctt tggcggcgat ttcggagttt cttaagaatt gcagcgccac gtacttgtcg
    87721 ttttcgctgg cttctggatc gtgaagaata gccgcaacct gtttctggtg tgccggacgg
    87781 agcatgaaag aagcgtcgtg aaaggcttgc tgcgccagta gggtcagggc ttctggttcc
    87841 actttcagga tggtttcgcc gtcgaagtcg gcaacgctaa cgtaatcgga agtgagtaga
    87901 tagtattcgg tattgtcttt ccccatcggg aaaggtgcct ggtagataaa gggtttgttt
    87961 gacatagctt ccagcctgta actctgtatt tgttattcga aaatggcgtg ccgcgtagtc
    88021 acgggcggca cgcaaagtgc atttataaga acccgtacat cgcggcgaag atccagccga
    88081 agacgcacga tacgctaaca ccaatcaacc ccggcagaat aaaactgtgg ttgatgacga
    88141 agcgaccgat gtgggtggtg ccggaacggt caaactgaat cgctgccaga tcgctcggat
    88201 aagtcggcag gatgtaataa ccgtagcaag ccggtgctga agccacgatg tatgccggat
    88261 caacgccgat cgccagcgcg accggaacaa tcgccgccag cgccgcagcc tgagagttta
    88321 caaacttgga aaccagcagc agaacaatgg catacgccca cggatactct ttcaccattt
    88381 cacccagtac gccctgaatt tcagacatat gcgcaccgaa catggtttct gccatccatg
    88441 cgataccgta caccgccacg atggcgatca taccggaacg gaagacttcg ttttttgaga
    88501 tagacgcggg attggttttg gtcaggataa taatcagcgc cccggtcagc agcataaaca
    88561 tctgaataac cagtaccatc gacagcggtt tgccgccgaa ggatggacgc aggtccgaat
    88621 cagcaccaag aagggcgact acagcgattg ccccgaggaa aatccacatt gccagccagt
    88681 tgcttttcgg cagtttttta tcgagcagcg tcgcggtatc accgtaaaca tactcacggt
    88741 tttccggtac ggagatgaat ttctggaact cttcgtcttt atccagatct ttaccgcgga
    88801 accagctgaa gataccgatc gccaggatac cgattaacgt cgatggaatg gtgattgcca
    88861 gcaggtcgag gaactcaaga tggcgaccat caaaggtgac attacccagc attgcaacca
    88921 gagacacgac cgcaaccgac accggactgg cgataatccc catctgtgca ccgatagaac
    88981 ttgccgccat cggacgttcc ggacggatgt tgttcttaat ggcgacgtcg tagatgatcg
    89041 gcagaatggt gtaaaccaca tgacccgtac cgcaaagaat ggtcagtgta caggtcacaa
    89101 acggcgcgac aattgagaca tatttcgggt tgcggcgcag cagcttctcg gcaatttgca
    89161 gcatgacatc aagaccgccc gaagcttgca aggtcgccga tgccgccacc accgcaatga
    89221 taaccagcat gacatcaacc ggtggtttac ctggctgaag gtggaagacg aagaccagaa
    89281 tgaccagacc gataccgcct aataaaccca gcgcgatacc accctttctg gcaccataaa
    89341 acagacatat cagtattatg ataagttgga tagtaaataa catgtgtgaa ccctcgcgat
    89401 aatcctattt aaatttttgc tgaatagatc acagtcacgt tctgttttgt atgaactgtt
    89461 ttcagagcct gactaaatat ccgtttggtc gttaccggct ttagcaaata cctcacagta
    89521 aatattggct gattaaagca gcggcaaatt tatgcactgt gataagcggc tttttcagag
    89581 agaaaaactc tctggcgatg ggtttatagt ttgtactgcg cagatgttaa tgttttgttt
    89641 gctgttatct tattgatatt tatcggtcta ttttttgttc tcagggcaga tatcattagg
    89701 tttaaagatt aatctgatct acccatttgt gggtaaaaat acacataatg cgggtgacat
    89761 aatagttaat taacttttgt tatcgttttg aaattaaaaa caccgttcac ctgaagagat
    89821 attaattttt agcgatgatg gagggataat tatatttgat ctggcacaag ttttactgat
    89881 gaaggatgta acttgtgcca ggggtatttt gcattacggt aataattatt acttacagat
    89941 aaccagcgat ccgttattgg caatattgtt tcagtagtga gtagtgttct gcctgaatac
    90001 ggtaacggta aaccggacgc cccgtgacgc cataatggat actggtgaac aagatgtggc
    90061 agttgaccag ccagatgagg tatttacggc aggaaacacg cgagatgtta acctcgttag
    90121 ccagctcgtc ggttgaaaac tcatagtcct gatgcgcgtc aatccactgg cacagcgtgc
    90181 gtaacgtctg cggcgttaag ccttttggca agcgacgagg atcctgttcg ttggagctgc
    90241 tgccgtggat tagctgatca agctcggcct ggtcataata ctgatgtttt tccagcgcca
    90301 ttttcttttg ccgccagccg gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga
    90361 tcaggtaatc cacgacaccg taatgcagcg aatctttaat ggttgccgca tcggctgcgg
    90421 aggagatgac aatcacatca cttttgcaac gcgcgttatg caggacaggc agtaaatcga
    90481 gcccgttctc tttttgcata tagatatcga gcaatatcag gtcgataggc gtatcgctat
    90541 tgaagataat ctctttggct ttctccagcg tcgaggctgt tccacagcat tgaaagcctg
    90601 ggatttgtgc tacgtatcgg cgattcagct ccgcgaccat tgcgtcgtca tcgataatta
    90661 atacattgat catctgttcg acctctcccc gtcccagggt atctggacaa aaaattgtgt
    90721 gaaaatcccg ggttccgatt ccacggcgat gctgccgccg agattttcta cctgttgttt
    90781 gacaagtgct aaaccgacgc ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat
    90841 gtgatcgatt ttgtcgggtg cgatccccgg tccatcatcg ttaacttcgc agtgcagcca
    90901 gccgtgacgg tagtgcaatg ttacgctaat ttcgccaccg ggttccggcc ctaatgcttc
    90961 cagcgcgttt tctatcagat ttcccaatgt ggtaatcagc gtcgcgacct ggtcctcact
    91021 gccgctgtct ggcagctggc tttcactgtt taaaatcagc gtatggccta aatcggtcgc
    91081 gcggttaatc ttgctgatta aaaaaccagc gataaccgga gatttgatct tacccagcag
    91141 agagccaatc tcttcctgat agttattggc tgttttgaga atgtaatctt ccaactgctt
    91201 ataactcttc agatgcaata atccgagaat cacatgcaat ttattcataa attcgtggga
    91261 tcgttcacga agtgcgtcag catagttgac cagaccatcg agtcgctgca tcagtttacg
    91321 tacttcagtt ttgtccctga aggttgaaat ggcaccgatg ataacgccat tactgcgcac
    91381 cggaacggtg ttgatcagta atagccgatc tttaatcgta atctcttcgt cgcggcgcgg
    91441 cgtaccgtcg cgtaacactt ccgagacatc taccacctgt gaccatgagt ggctgagcgt
    91501 cgacagtttc tcatcgtcct gcgacttacg gtaattcagc aattcttgtg cggcatcgtt
    91561 gatcagcgtg acctcgccgc gatcgtccac ggcaacgacg ccttctttga tagactgcaa
    91621 catggcctgg cgctgctcaa acagcgtgga gatttcgtag ggttccaggc cgaaaaggat
    91681 ttttttcagt accttaacca gaatgcaggt gccaatcagt ccgaccagca tgccaaataa
    91741 taccgaccag ataatgctcc agcgactgtc attgatttgt tgggtcacgc ggcttaactc
    91801 aaggccgatc gccaccacgc caatttgttt atgattttca tcgtagatgg gggtaaatac
    91861 gcgtaaagcc tgcgccagaa aaccgcgatt gatagcgaca ttttctttgc cattcagcgc
    91921 tttaaggatg tcatcacctt taaatggctg accaatacgc tgggcttcag gatgcgagta
    91981 gcgaagactt tgcatatcgg taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc
    92041 ttccgcgatg gcctggattc cgctctcctg cggttttttc tgcaagccct gacggatttc
    92101 cggcgagtcg gcgagggtac gcgccactgc cagtgccttg ttggctagcc cgtctcgcgt
    92161 catatcactg atttgcgaga agtaaatcag atgcaccacc aatagcaccg agaacagtac
    92221 cgcactgacc attaagatca ctgtggtact caatttcatc ggacgtttgc gtaacatgcg
    92281 gtagggcaat gaatgtctca tcagcttcct tgtgtgacaa atttcttaag cattatctct
    92341 gatgaggcgg gtaattcaaa gggagtaaga atgattggct atatagggga agagactctg
    92401 gcaacggaaa ctgccagtgc tgtatgaaga ttccagggct atgcttatag cgataatcat
    92461 actgatgaga gagggaaagt catggatcag gcgctactgg acgagggtta tcgctgttat
    92521 accggcgaaa agatcgatgt ctatttcaac actgcgatat gtcagcattc tggcaattgc
    92581 gtacgtggca acggcaagtt atttaatctc aaacgaaagc cgtggatcat gccggatgaa
    92641 gtcgacgtcg tcactgtggt taaagtgatt gatacgtgcc cgagcggcgc gctgaaatac
    92701 cgtcataaat aagcgagggt aaaatggaaa tacgcgaagg ccacaataaa ttttacatta
    92761 atgacgaaca aggcaagcaa atcgctgaaa ttgtctttgt gccgaccgga gagaatttag
    92821 cgattatcga acataccgat gtcgatgaaa gcctgaaagg gcaagggatt ggtaaacagc
    92881 tggttgcgaa agtcgtggaa aaaatgcgtc gggaaaaacg aaaaattatc ccattatgtc
    92941 catttgcgaa acacgaattt gataaaacgc gggagtatga tgatattcgc agttgatggg
    93001 agagtacaga gtcacgatat ttttcttgct ctccgcggag tgatgcagga gagcatctga
    93061 agggtagggg gatgcacaaa gaatgggcag agagcgcgtt tttttgtccc aagtcatccc
    93121 ctttactgag caaaaaaaag aatatctcct atatgagaat catcaatcgg ggttaataag
    93181 ttttgcgtcc ccagagcatt caatattgat aggagtcata ttatggaagg taaaaacaag
    93241 ttcaatactt atgttgtttc ttttgattat ccatcatctt attcctcagt gttcttaagg
    93301 ttaagatcat tgatgtatga tatgaatttc tcctctatcg tggctgatga atatgggata
    93361 ccacgacaat tgaatgaaaa ctccttcgca ataacgacat cgttagccgc aagtgaaatc
    93421 gaagatttaa tcaggcttaa atgcttagac ttaccggata ttgattttga cctcaacatt
    93481 atgacagttg atgactattt ccgtcagttt tacaagtagc caggaaggat aagaagaaca
    93541 taaaaaatgg cactattctc taaaatatta attttttatg tgattggtgt gaacatatcc
    93601 tttgtcatta tctggtttat ctcacatgag aaaacacata ttcgtttact tagtgcattc
    93661 ctggtcggaa taacctggcc aatgagtctg cctgtggcat tacttttttc tctcttttag
    93721 gagctgtagt tgagtggacc cgctggttat cgtgaaacga agcgtctctc ctcaaaactg
    93781 gatttgcgga acgacgaacg aagcagtcgc ctgactgctt cgttcattaa agtgaaattt
    93841 atttctgtgg gcgcatcgcc gggaagagaa taacgtcgcg aatagtatga ctgtttgtaa
    93901 acagcataat cattcggtcg ataccaatac ccagaccagc ggttggcggc agaccatatt
    93961 ccagcgcagt cacgtaatct tcgtcataga acatggcttc gtcgtcacct gcagctttag
    94021 cgttaacctg ttcctggaaa cgttcagcct gatcttctgc gtcgtttaat tcgctaaaac
    94081 cattaccgat ttcacgacca ccgatgaaga attcaaaacg gtcggtgatt tccgggttaa
    94141 catcattacg gcgcgccagc ggtgacactt ctgccggata ttccgtaata aaggttggct
    94201 gaatcagatg tgcttctgcc acttcatcaa agatctccgt gacaatacgt cccagccccc
    94261 agcttttctc taccgtaatt ccgatagatt cagctaatgc tttagcagca tcaaaattat
    94321 ccaggtcggc catatcggtt tctggacgat attttttgat tgcttcgcgc atggtgagtt
    94381 tttcaaacgg tttgccgaaa tcaaacacat gctcgccata agtgacttta gtggtaccca
    94441 gaacctcttg tgccagggtg cggaacaggg actctgtcag ttcaatcaaa tcgtggtaat
    94501 ccgcatacgc catgtagagt tccatcattg tgaactcagg attatggcga acagaaatac
    94561 cttcattacg gaagttacgg ttgatttcga atacccgttc aaaaccgcct acaaccagac
    94621 gtttcagata cagctccggc gcgatacgca ggtacatatc taaatccaga gcgttatgat
    94681 gggtaataaa cgggcgagca gatgccccac ctggaattac ctgcatcatc ggggtttcta
    94741 cttccataaa gccgcgcgcg accatgaact ggcggatagc ggccagaatt tttgaacgga
    94801 caacaaacgt ttgacgggat ttatcgttag cgatgaggtc cagataacgt tgacgataac
    94861 ggacttcctg atcctgcaga ccatggaatt tatctggtaa aggacgtagt gcttttgtca
    94921 gcaggcgcaa ctcagtacag tgaatggaaa gctcaccggt ttgcgtctta aacagcgtac
    94981 cgcgggcacc gataatgtca cccagatccc attttttaaa ctgatcgtta taaacacctt
    95041 ctggcaggct atctcttgca acgtacagtt gaatacggcc accgacatcc tgcaacgtta
    95101 caaaggaggc tttccccatg atacgacggg tcatcattcg gccagcaacc gagacttcaa
    95161 tgtttaagga ttccagttcc tggttatcct tcgcatcaaa ctcttcgtgc agctggtcag
    95221 aggtatggtc gcggcgaaaa tcattgggaa acgccacacc ttgctgacgt agtgccgcca
    95281 gtttttcgcg gcgatttctc agttcatcgt taaaatcaat agcctcattg gctccccgtg
    95341 tttcttgttc agacattttg gttcctctaa atccagcttt caatttagcg tagataaaga
    95401 gacagatcgg tacactgtat tatctgcctc gactattaat aactcagttt atcttatcga
    95461 aactaacgct tacctcaagt tgatatcact aataaataac catcaaaatg gtttaaatga
    95521 cttattaatc tagatttgct agtgttataa actgaaagtt aatttattca taaccaattg
    95581 tttttacgac agttaatcgt tgctctcctg tatcatattc gtcggcgtgc taaacagaaa
    95641 acgggtagca aaggccaata cgacgatgat cgcgacacaa gccaacgtcc actctcccat
    95701 ctgagaaaag aatcgctggt aagctgcaat tgccataccg ctaatttgcg actctgtcgt
    95761 ctgctgtgcc acgacgcctg ccagccagtt ggcgaccgcg cctgttgcca gcatataaat
    95821 accggttaat acgccagaca ttttcagacg cgtgatttgc gcaatcgcca ccgggtcaat
    95881 aaagagttcg gcaaagccca ttagcgccaa cccggatatc atcacgccca ttgacgcttg
    95941 accgtcagcc gctgcatgtc gggcatcaaa tgccaacaac ataaagccac aagccatcag
    96001 cagtaagcca aaggcaaact tcagccagac gcgcaatgtt gagttgccgc ggctttctgg
    96061 cgacgccagc caggccagta caaccccagc gagcatcacc gcaattgcat tcaccgactg
    96121 gaatagtgct gtaggtactt caatattgaa tgcctgacga ttcacaaagc gatcgataaa
    96181 caagctgatg gtactgccgc cctgttgtgc cagtacccag aacaatgtcc cgacaaacat
    96241 caacaataca atttgccaaa gagcacggcg gtgttcgggg aatttgacca tcatgcgggc
    96301 aatgatttgt gcggcaatga ggcaaacgat cgccagcaaa tatccggacc agtcgttctc
    96361 cagcagcagg gtaaaaaata ctggggctaa acagagcatc accactaacc agctccatac
    96421 cggtaaggca aatttgacgc tagtgagcgc ttttttatcc atactacgcg tggactggaa
    96481 atgacgatgc ccgcttaaga aaatcaacaa accgataaac atgccgccac ccgcaagggc
    96541 aaagccaaca tgccatccat accactgagc agccaggccg caggcgattg gggcggcgat
    96601 agaaccgata ttgcccgcag catacagcag cgaaaaaccg ccatcacgtc gatgatcgtt
    96661 ctcgtcgtaa agctcgccaa gcaaacagct gatgtttgat ttgaataaac cgtagccaca
    96721 aataatgatt gccagcgcca gatacaggct aaaggttgaa tttgtatcaa tgcccagcac
    96781 cacatggcca agggtcatta acagcgcgcc ggcaatcact gcagtgcggt tgccgagcag
    96841 gcggtcggca agccagccgc cgagaatagg ggtaacgtaa accagagaag catatgcgct
    96901 gaacaggctg atggcatggt tatcatcaaa accaagctga tgggtgagat agagaatgag
    96961 taaggcacgc atgccgtaaa aactgaagta ctcccagatt tggatcgcca cgatatagta
    97021 tatcgcgcgc ggctgtgagg gtgttttcat gtgttctcct tatgagcaaa aaagggaagt
    97081 ggcaagccac ttcccttgta cgagctaatt attttttgct ttcttctttc aataccttaa
    97141 cggtatagcg gccatcagcc tgacggtatg caccgtgaat atcggtttca aagcccggat
    97201 agtgagcgcc gatttcacac agcatctgca ggaactccag aaccggacgg ctttcttcgg
    97261 tgatcatttc acccggcatt accagaggaa ctcccggcgg atatggaagg atcatattgg
    97321 cgttaatacg acctaccatt tcgtcgaggt aaacttcttc ggtcataccg tgcagctctt
    97381 tctggaacgc agcatacgga gtcattacca tcgtcggcag cacttcaaat gcgcgataca
    97441 tcagatccgg cagattgtgg tgaacaatca gtttgtggat attctgagcc agttcctgaa
    97501 tacgcatgtt ttcatagaat tcaggatctt cacgatacag agacggcagc atgtttttca
    97561 cacgcaggtt caggtcgaac gcacgtttga agtcagtcag agcacgcagc aggctcagtg
    97621 ctttggtctt atcgataccg atgctgaaca ggaacagcag gttatacgga ccggttttct
    97681 caacaacgat gccatgttcg tcgaggtatt tcgccacgat gctggccgga ataccaaagt
    97741 cgctcatggt gccgtctttt tccatccccg gagtcagcag ggtgactttg atcgggtcaa
    97801 gatacatgtg ctcgttatcg atgtttttga agccgtgcca ggtgctgtca gaacgcagcg
    97861 gccagcattc agtggtatcg atatgatccg gctgccatac atcaaagaac cagccatcag
    97921 attccgttct cagacgtttg atctctttac ggaatttgat cgcacgttca atggaaccgt
    97981 tgatcagacg cttaccagca ttgcctttca tcatcgccgc tgcggtttca gtggacgcca
    98041 cgataccgta gtgcggagaa gtggtggtgt gcatcatgta ggcttcgtta aaggtttctt
    98101 cgtttacgtc acctttaacg tggatcatgg aagcctgaga gaacgccgcc agcagtttgt
    98161 gagtggactg ggtttcgtaa atcactttcc cttctacacg gccaccgctc ataccgcatt
    98221 taccttcgta aatcggtgag aagttggtgt aaggcaccca cgcggagtca aagtggatgg
    98281 atttcacatc cagtgttttc ttgataaagt cggtgttgta cagcagacca tcataggtag
    98341 agttggtaat tacagcatgt accggccagg ttgcgtttgg tgtttctttc acgcgcttag
    98401 caatggtagc gtgctggaat tcactctgtg ggataccacc aagaataccg taagcgttac
    98461 gggtcgggcg gaaatagatt ggcgtaacat cgctcatcat catcaggtgg gtcagcgatt
    98521 tgtggcagtt acggtcaatc agaatggtgc tgcctgctgg agcagagtac ataccaacaa
    98581 ttttgttcgc agtggaagta ccgttggtca ccatgtagct gcggtctgcg ttaaagacgc
    98641 gagcgatata ctgttctgct tctttgtgtg gaccactgtg atccagcaga gaacccagtt
    98701 cagatactga aatggaaata tcagatttca tggtgttcgg accaaagaaa tcatagaaca
    98761 ggctacctac cgggcttttc tggaatgcag taccgcccat gtgaccagga gtacagaaag
    98821 tatatttacc ttcacgaaca tatttaaaca gtgctttagt cagcggaggc agaatagtgt
    98881 tgatatattc gtcagtggtc tgcttgatct tattagcaat atcttcagca gcacccagcg
    98941 catattcaaa gaagctaatc tgtaaacgca ggtcattcag gcttacatcg agagtggaat
    99001 acgtattagc gaacgcgtac aacggcaggt tctcgttcat tttgctaatt tcttcgcaca
    99061 gctcgagatt atatttatcc cagtcaaaaa taacgccgca cagacgcgca ttgttttcga
    99121 tcagttttaa taagtcgtca cggtcgttcg ggtaaacaat ctggaagttc agacgttcaa
    99181 gcgcgcgatg aagttcacgg atgggttctt ctttaaaata aacccccatg tgattcaata
    99241 ttgcaataac gttcatagtc atatctccag gtaaaaaagg cccctcccaa cacatgggac
    99301 aaaatgaaag gaggagcctc ggaaaatact tttaattaat gtgcgttaga cgcggtgtgg
    99361 ttatccattg agtggctctg gcgctcgtgc attttgcgag cgtagaacat caggataatc
    99421 aggctgacga tgaaggtacc tgccagctcg aaggagcttg cgcccatcag cgcgatgaag
    99481 cagaacacac aacccagtac agagcagatc aggctgacaa agttgcggat gttaacgcct
    99541 tcaaaacgaa tcaggtcaac gcaagagtag aagtacggca gcatagtcag cagtactgcg
    99601 ataccggtca gttcaccgaa caggtcagat gctttaccac cggcagagtt catcagagtg
    99661 atgaggatca tcagggcagt cattttcact gcagccagca gcagaccttt tttcggaata
    99721 ccgttgctgt cgacttcacc ataaactttc gggaagttac cgtcgttagc ggcacgtaca
    99781 cctgcctggc ctaccaacat catccaggag cccagagaag tcaggcacgc aaaggcggtg
    99841 aatgcagaaa ccagcggcgc agcccagtta ccgaggatgg ttgaagcact gattgcaaac
    99901 ggagcaccgg aagctgccat tacagaagat ggatacatac cggaaagcac ctgagtcgca
    99961 gcgatgtaaa caatacctgc taaaccagta cccagcatgg ttgccagcgg aacggtacgt
   100021 ttcgggtttt taaccatacc agtacttact gctgcggatt caacacccac gaaggcccac
   100081 aggcagagca gaatactttt aatgatcgca tgaccatcag tggtatccgc agtattccag
   100141 ttagctgcat aagttgccgc atcaaaccaa tgccagccaa caatagcggt cattactacc
   100201 ggaataagaa ccagcaccag accaatagtg gttaaacggc ttacccaagt accgccgagc
   100261 atatttacaa aggtaaatac ccagacgata gcaatacagg cgatacccgc cggaacagga
   100321 tcatttaata ctgggaagaa ggtggaaaga taagataccg cggtaatacc aatcgccagg
   100381 ttaccaatcc agttagcatg gtaataaaga acacctgtct gaaaaccaaa tgcaggggaa
   100441 atttctccgg cataagcaat tgggccacct tgttgcgggt tttttgttgc cagtcgggca
   100501 tatacatacg ccagcgacat tgcaccaata atagagataa tccaacccca gatagcaata
   100561 ccaccgatac ttgctaggtt cgcaggtaat aatgcaatac cgctccccat catattaccg
   100621 gcaacaacac cggtacaggc aaatagcccg atcttcttgg cagaactcat gctcttctcc
   100681 taatttcatt tttgaatttg gagtccgggt catgatgtat aactatttcc tgaccagacc
   100741 aaactggcgc taagaatact cacgaaaaaa ggattaatcc taaagattag gtgaaataac
   100801 acaaaagttt ctgtaagtga gaacttgagg ttttttatta acacatcagg atcgcaagtt
   100861 gatatcatga aaagataaac attcaatgtt tacaatggat tgcgggatat tctctggtta
   100921 aatttatgtg ataaaaatta tgcggcaaat aaattgccgc aacatattat accaatagaa
   100981 acatacaaaa actcaacaac aaatattccc gagcataaat caacccggga gttacttatt
   101041 ctgaagcgag aaatttgtcg agataaggta caacataagg aacagaagtc tggaatatac
   101101 cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa attaaaggcg gtgagatatg
   101161 catcagccgc ttcccggttc atccccttca tttcataaac cttgccaagc aacacataat
   101221 ttagccagga catttcaaga tcaatgccag tatttatcgc ctggtaagac tcatctgttt
   101281 taccttttac cagagcactg accgctttta tttgatatat aatggacagg ttattcaatt
   101341 ccggcagtgt aacaatgttg tctatttctg tgttcagtgc tgctaattgt ttttcatcta
   101401 aaggatgttg agaatggcgc acgatatcaa ctaatgcttt ttctgctctc gcgtaggtaa
   101461 attctgggga tgattgaaca atctcaccta ataattcact ggcacgattc aatgatttat
   101521 catcgccatg cagtaaataa tcatgtgcct gataaaaatt agttaataac gcaccacgat
   101581 gaggcaatat ttgctggagc gtctcctgca ttcgttgtgg ccacggttgg tttaacgctt
   101641 ttgataaact ctccagtaaa tcattttgaa tcgccagctg attaccgtta gtgatgacat
   101701 aacgtttatc cagcatggtt gaaccatctg cattgtctac caattttatc gacataaagc
   101761 attgttgagc acggtattgg cgctgattaa caaacgcaat agataatgtt ttaccggaac
   101821 tgctcggttc atcaatgttg tagttgattt tgtcatgcac cataaaggtg gagaaggtgt
   101881 taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta agagagctga tacggggaac
   101941 tccagctgtt acaactttta tttaccatat taatgtcaat atcgcgtgga ttgagcaaaa
   102001 tacgcgattt gctcatagga agacgtgtat caagagttga aaacgctacc agtgctacac
   102061 agatacctaa cgacaacagg aaaaaaaacc atacccaaaa ggtagtgaat cgtttgcttt
   102121 taactgggga ttgttcaggt ggcgttgcgg tgttttgaat gttaagactg tgggagggag
   102181 aatctgtggc aggaaccgcc tctggtatag ggggaggcga agatagcatt atttcctctc
   102241 cctcttcttc gctgtaccag ataaccggca ccattaattt atagccgcgc tttggtacag
   102301 tagcgatata gacaggacta tcttcatcat tatcttttaa tgacttacgt agttctgaga
   102361 tactctgcgt cacaacatga ttggtgacaa tacttctctt ccagacatta tcgataagtt
   102421 catccctgct aagtacttcg ccactgtgtt gagcaaagaa aaccagaaga tcgattaatc
   102481 tcggctcaag ggtaagttga cgcccattgc ggctaatttg gtttatggac ggagtaacaa
   102541 gccattcgcc aacgcgaact acaggttgtt gcataataga aactcattcg aaaagggaat
   102601 gatgcaatga taattgccac aacctatttt taccatctat agatgggttt atttacatat
   102661 tattggtgaa tgcaagacgt tatttttacc agccataaac ttcttgatta catagtatta
   102721 cgaaaggatt ttactgagaa ccagaagtaa tattccttac catcaaaatt catcatcttt
   102781 gccaaagaaa aatgttcaga aaataatcca tggaaaattg tccggagcgc ttactatttt
   102841 aatggattgt tagtctttgc atgagcaagc ggactgatac atttctcttt gttctcattc
   102901 agaaaatctc atcagtcggc gttcagaccg gtgttgtgct ttatggatgc gttacgctcc
   102961 tgatgacgtc atttgacgtt caacaacatc acggggccgc acgacatttc acgtcagtta
   103021 gtgctatcgc tcaggaacaa attttcccga attgggatat gcccgcaaat tgctggtgat
   103081 gtggggagaa tctggttgag ttcgggagaa ttgatttgga ggcagaacgc ttaaattgtg
   103141 gcgtccagaa acgaaaaacg gacctccgtg gaggtccgtt gatatgaatt tggtgccgga
   103201 ctcggaatcg aatatggttg ttatcatgtt gatattaatc tcgtttattt ggtttgatga
   103261 aaaatgtacc ctctcttgta ccctcggtcc cttctggtgg tttttatgag aacaagtaga
   103321 tcaagatttt tgtcatcaga acacgtgtat ttttgtgggt tttattcctg agttttattc
   103381 ctttttacat cgctaacgct ccgggatagt gcttggcaat aatatcatta agcctgtcaa
   103441 ccagttctgg tttcctgaac gtgatgtgtg cggagccctt cttaaagtat ctgatgctga
   103501 acatctcatc ttcatagtcc tctttaccct gaacagactg gatgtggtca tccagatgaa
   103561 tggtgatgtt ttcccggttg tcggggatcg gtttgccgct gaacaggtgc agcattcttt
   103621 ccagatcggc aagtcgatcg gcctgttgcc cggtgataag atgaaacccc catctgtccc
   103681 accttaccag gttgttgacg ataattttac tgccaaactt gcaggggcaa ttggttttgt
   103741 aattccagct cagtcccctg aagacgttga tcatcccccg ctcaaacact tcatctttgt
   103801 tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc actgatttcc ggaaaattat
   103861 cgtactccag tgactggtac catgtatcac gggcctgtgc atccattaag gaaagcatcc
   103921 ctgaacgttg catcaggtca cgccagattt cgcggtcaag attccgggtg atggctttca
   103981 ttgcggtttc cggtttctcc atcagccagc agccgcagcg aaaatcctgt ttcatggccc
   104041 aggccggggc ggttttaccg ccaatacttt ctgtcattgc tgagatttca gccagttgcc
   104101 ggataagtgt gtcgatttgc agcagcgcac tctttcgtac agccaaaatg tggcggatac
   104161 tggttgagca gataacgtcc gtatgtctgt tgaccagttc cgggcgggag attgtgatat
   104221 ctgacatcat cgtttttcct tttggtttat aaacgaaaac gccagccgta ttcaggctgg
   104281 cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt ctgttgcaga agctgcagtt
   104341 ggcgcagggt atggatatcc tccctcgcct gttcaatgca gggcagcagt gctgccgaat
   104401 ctgttttatc ctcatcgttg tcaaagataa ttcccaattc gcagtcgata ttgtcctgca
   104461 gccacgcaat cagaatatcc agtgcggttt ccgtggttaa tgatttcatg ttgttcctgg
   104521 ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca gatgaatgag
   104581 actgacgcca taacgtcgga tgagtgtgac aaccagacgg cggaacgttt ttgacaggcc
   104641 ctggcgctta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
   104701 gcctgtaaaa tgaatccatt ctgattcgcc ggtgtcattc gtctgatgcg acagcgagta
   104761 cagccaggcg ttgtcttttg ccgtgatatg cgcggtactg cagcgtatgc cagtcagggc
   104821 agcaaatgat ggcgtgcagt ctgttgaggc tcggtcagat tcatcaacaa cacgaaggga
   104881 gtgaccgtta tcgcagacca cgttaatcag ttcagcaagc tcaataccat cgacgtcaac
   104941 gacaatgcgc cccatgttca gtgcctgtac gttaacgctg tcggcttccg gtgtcaggcc
   105001 agtttcattg tttcgcccct ggatgtttac ccagggtaat gttatttacc gttctttaat
   105061 tgtcgcgagt catcaggcct gttgcccggc gagcccggag gatatcgatg ctgtttatta
   105121 actgagagtg ggtacaggcg ctgaatcccg actggtcggt acgtaccagc gcgtattttt
   105181 caacgagaaa gttcaccgcg tcacacagtg aaatgcctgc ctcaatatgc tgctcaatca
   105241 cacgttcatc agcgaacggt gtgtcattca gcgtgaggcc gtagtgctgg tccagcagtc
   105301 gggtgagcag tgtctgccag atgatgacgg gagacgggca gcgcgatgcc tcccgtacgt
   105361 gtgtatcggg taatgttttc atactgaagg ttttcctgat atgcagagat gagaaatggg
   105421 aaagtggcgt ggtgaaaaca ccaggccgta gcagaaggct attctggaga gttaattttt
   105481 catttcgggc gtcggataaa cagccagata aacgtaacca caggagccca gggtatcggc
   105541 ttcgcaggtc agtcctttcg catacagcgt gacggtatgc tgatagcggg ggctgagttc
   105601 accactggtg agcatgagct ccagttgttt catcagcagc ggaaaggcct ggtccaggtg
   105661 gtacgcatct gcatcgctga acaggcctct gataccggcg cggtcggcaa ggtaatgcaa
   105721 ccggttaccc tcctgcacca gacagacgcc gaaacagggc gtcactgtgc agggtagtcc
   105781 ccaccaggga tggtcgtgat tgtcatcggg atgcgttgtc ccggagagtg tgtctgacac
   105841 gataaaatcc tcacagaaaa tcggtgaatg atagtttaac gatgacgggt cagccggcgc
   105901 gcagaccggc ttcggtaaag gtgtattcgt tgatgagcag gacctcatcc acagcggcgt
   105961 ctgagctcag ccagtcgtac tcgttttccg ggtgacggaa catcagttca gggtgacgct
   106021 catcagccgg acatacaggc caaaactgtc cttacggcgt tcggcaaaca cagccagcac
   106081 gccgggaata tcctgcactt cacgacaggt atgtgcttca gcgctgccgt gccagcggta
   106141 cttaccggta cagaacggaa aaagacggga tgtcggatgt tgttggtgaa tacgcatggc
   106201 ttcaccacgg gtgataattt tcataatggg atacctctga agacagaaga taaaagtgaa
   106261 aacaggtgtg atgtggttgt gacgggttaa agcagaccat gttcggcaaa ggagaaaacc
   106321 tggttgccac cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt
   106381 accagacgtt cggtgataag ccggtctgcc ttactgggtg tgacttcacc ggacgggtga
   106441 ttgtgtgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg
   106501 ggatggactt ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga
   106561 ttctggttat tcagatacag cacccggaat tcttcacgct ccagtcccgc catgttcaga
   106621 atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc atgaagatgg
   106681 cggtccaggg ttttcagggc c
//
LOCUS       DAHBHC010000007        94013 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHBHC010000007 DAHBHC010000000
VERSION     DAHBHC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 94013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.007"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1138)
                     /gene="uhpC"
                     /locus_tag="M2A35_000646"
     CDS             complement(<1..1138)
                     /gene="uhpC"
                     /locus_tag="M2A35_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCB7196988.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAG"
     gene            complement(1148..2650)
                     /gene="uhpB"
                     /locus_tag="M2A35_000647"
     CDS             complement(1148..2650)
                     /gene="uhpB"
                     /locus_tag="M2A35_000647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCB7196989.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(2650..3240)
                     /gene="uhpA"
                     /locus_tag="M2A35_000648"
     CDS             complement(2650..3240)
                     /gene="uhpA"
                     /locus_tag="M2A35_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCB7196990.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(3315..3605)
                     /gene="ilvN"
                     /locus_tag="M2A35_000649"
     CDS             complement(3315..3605)
                     /gene="ilvN"
                     /locus_tag="M2A35_000649"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCB7196991.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(3609..5297)
                     /gene="ilvB"
                     /locus_tag="M2A35_000650"
     CDS             complement(3609..5297)
                     /gene="ilvB"
                     /locus_tag="M2A35_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCB7196992.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(5403..5501)
                     /gene="ivbL"
                     /locus_tag="M2A35_000651"
     CDS             complement(5403..5501)
                     /gene="ivbL"
                     /locus_tag="M2A35_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCB7196993.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            6066..6155
                     /gene="tisB"
                     /locus_tag="M2A35_000652"
     CDS             6066..6155
                     /gene="tisB"
                     /locus_tag="M2A35_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCB7196994.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            6574..7758
                     /gene="emrD"
                     /locus_tag="M2A35_000653"
     CDS             6574..7758
                     /gene="emrD"
                     /locus_tag="M2A35_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCB7196995.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(7766..8263)
                     /locus_tag="M2A35_000654"
     CDS             complement(7766..8263)
                     /locus_tag="M2A35_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCB7196996.1"
                     /translation="MTGSQVIDAEEDRYKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(8260..8622)
                     /locus_tag="M2A35_000655"
     CDS             complement(8260..8622)
                     /locus_tag="M2A35_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCB7196997.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(8612..8959)
                     /locus_tag="M2A35_000656"
     CDS             complement(8612..8959)
                     /locus_tag="M2A35_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCB7196998.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            9068..9517
                     /locus_tag="M2A35_000657"
     CDS             9068..9517
                     /locus_tag="M2A35_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7196999.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(9564..11057)
                     /locus_tag="M2A35_000658"
     CDS             complement(9564..11057)
                     /locus_tag="M2A35_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCB7197000.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWCKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(11054..12769)
                     /locus_tag="M2A35_000659"
     CDS             complement(11054..12769)
                     /locus_tag="M2A35_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCB7197001.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            12936..13829
                     /locus_tag="M2A35_000660"
     CDS             12936..13829
                     /locus_tag="M2A35_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCB7197002.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(13826..14002)
                     /locus_tag="M2A35_000661"
                     /pseudo
     CDS             complement(13826..14002)
                     /locus_tag="M2A35_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(14002..14640)
                     /locus_tag="M2A35_000662"
     CDS             complement(14002..14640)
                     /locus_tag="M2A35_000662"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197003.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            complement(14640..16256)
                     /locus_tag="M2A35_000663"
     CDS             complement(14640..16256)
                     /locus_tag="M2A35_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCB7197004.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            16552..17268
                     /locus_tag="M2A35_000664"
     CDS             16552..17268
                     /locus_tag="M2A35_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCB7197005.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(17265..18926)
                     /locus_tag="M2A35_000665"
     CDS             complement(17265..18926)
                     /locus_tag="M2A35_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCB7197006.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19123..19551)
                     /gene="ibpB"
                     /locus_tag="M2A35_000666"
     CDS             complement(19123..19551)
                     /gene="ibpB"
                     /locus_tag="M2A35_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCB7197007.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(19663..20076)
                     /gene="ibpA"
                     /locus_tag="M2A35_000667"
     CDS             complement(19663..20076)
                     /gene="ibpA"
                     /locus_tag="M2A35_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCB7197008.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(20070..20142)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20382..20714
                     /locus_tag="M2A35_000668"
     CDS             20382..20714
                     /locus_tag="M2A35_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCB7197009.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(20716..21930)
                     /locus_tag="M2A35_000669"
     CDS             complement(20716..21930)
                     /locus_tag="M2A35_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCB7197010.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            22031..23095
                     /gene="cbrA"
                     /locus_tag="M2A35_000670"
     CDS             22031..23095
                     /gene="cbrA"
                     /locus_tag="M2A35_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCB7197011.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(23092..24417)
                     /locus_tag="M2A35_000671"
     CDS             complement(23092..24417)
                     /locus_tag="M2A35_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7197012.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVTYFIAIFGW
                     SVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQ
                     FVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDY
                     IRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPN
                     YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLL
                     ISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAG
                     GLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(24492..25640)
                     /gene="dgoD"
                     /locus_tag="M2A35_000672"
     CDS             complement(24492..25640)
                     /gene="dgoD"
                     /locus_tag="M2A35_000672"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCB7197013.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(25637..26254)
                     /gene="dgoA"
                     /locus_tag="M2A35_000673"
     CDS             complement(25637..26254)
                     /gene="dgoA"
                     /locus_tag="M2A35_000673"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCB7197014.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(26238..27116)
                     /gene="dgoK"
                     /locus_tag="M2A35_000674"
     CDS             complement(26238..27116)
                     /gene="dgoK"
                     /locus_tag="M2A35_000674"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCB7197015.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(27113..27802)
                     /gene="dgoR"
                     /locus_tag="M2A35_000675"
     CDS             complement(27113..27802)
                     /gene="dgoR"
                     /locus_tag="M2A35_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCB7197016.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            28080..28736
                     /locus_tag="M2A35_000676"
     CDS             28080..28736
                     /locus_tag="M2A35_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197017.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(28782..29594)
                     /gene="yidA"
                     /locus_tag="M2A35_000677"
     CDS             complement(28782..29594)
                     /gene="yidA"
                     /locus_tag="M2A35_000677"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCB7197018.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(29709..30107)
                     /locus_tag="M2A35_000678"
     CDS             complement(29709..30107)
                     /locus_tag="M2A35_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCB7197019.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(30347..32761)
                     /gene="gyrB"
                     /locus_tag="M2A35_000679"
     CDS             complement(30347..32761)
                     /gene="gyrB"
                     /locus_tag="M2A35_000679"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCB7197020.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(32790..33863)
                     /gene="recF"
                     /locus_tag="M2A35_000680"
     CDS             complement(32790..33863)
                     /gene="recF"
                     /locus_tag="M2A35_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCB7197021.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAQYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(33863..34963)
                     /gene="dnaN"
                     /locus_tag="M2A35_000681"
     CDS             complement(33863..34963)
                     /gene="dnaN"
                     /locus_tag="M2A35_000681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCB7197022.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(34968..36371)
                     /gene="dnaA"
                     /locus_tag="M2A35_000682"
     CDS             complement(34968..36371)
                     /gene="dnaA"
                     /locus_tag="M2A35_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCB7197023.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            36668..36748
                     /locus_tag="M2A35_000683"
     CDS             36668..36748
                     /locus_tag="M2A35_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197024.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            36978..37118
                     /gene="rpmH"
                     /locus_tag="M2A35_000684"
     CDS             36978..37118
                     /gene="rpmH"
                     /locus_tag="M2A35_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCB7197025.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            37135..37494
                     /gene="rnpA"
                     /locus_tag="M2A35_000685"
     CDS             37135..37494
                     /gene="rnpA"
                     /locus_tag="M2A35_000685"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCB7197026.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            37458..37715
                     /gene="yidD"
                     /locus_tag="M2A35_000686"
     CDS             37458..37715
                     /gene="yidD"
                     /locus_tag="M2A35_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCB7197027.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            37718..39364
                     /gene="yidC"
                     /locus_tag="M2A35_000687"
     CDS             37718..39364
                     /gene="yidC"
                     /locus_tag="M2A35_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCB7197028.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            39470..40834
                     /gene="mnmE"
                     /locus_tag="M2A35_000688"
     CDS             39470..40834
                     /gene="mnmE"
                     /locus_tag="M2A35_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCB7197029.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPSTVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            41077..41151
                     /gene="tnaC"
                     /locus_tag="M2A35_000689"
     CDS             41077..41151
                     /gene="tnaC"
                     /locus_tag="M2A35_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCB7197030.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            41372..42787
                     /gene="tnaA"
                     /locus_tag="M2A35_000690"
     CDS             41372..42787
                     /gene="tnaA"
                     /locus_tag="M2A35_000690"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCB7197031.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            42878..44125
                     /gene="tnaB"
                     /locus_tag="M2A35_000691"
     CDS             42878..44125
                     /gene="tnaB"
                     /locus_tag="M2A35_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCB7197032.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPLCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            44257..45432
                     /gene="mdtL"
                     /locus_tag="M2A35_000692"
     CDS             44257..45432
                     /gene="mdtL"
                     /locus_tag="M2A35_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCB7197033.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            45407..46366
                     /gene="yidZ"
                     /locus_tag="M2A35_000693"
     CDS             45407..46366
                     /gene="yidZ"
                     /locus_tag="M2A35_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCB7197034.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            46523..47272
                     /locus_tag="M2A35_000694"
     CDS             46523..47272
                     /locus_tag="M2A35_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCB7197035.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLSIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            47294..47860
                     /gene="yieF"
                     /locus_tag="M2A35_000695"
                     /gene_synonym="chrR"
     CDS             47294..47860
                     /gene="yieF"
                     /locus_tag="M2A35_000695"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCB7197036.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(47914..49251)
                     /gene="adeP"
                     /locus_tag="M2A35_000696"
     CDS             complement(47914..49251)
                     /gene="adeP"
                     /locus_tag="M2A35_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCB7197037.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            49418..50083
                     /gene="yieH"
                     /locus_tag="M2A35_000697"
     CDS             49418..50083
                     /gene="yieH"
                     /locus_tag="M2A35_000697"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCB7197038.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            50150..50623
                     /locus_tag="M2A35_000698"
     CDS             50150..50623
                     /locus_tag="M2A35_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197039.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            50672..51259
                     /locus_tag="M2A35_000699"
     CDS             50672..51259
                     /locus_tag="M2A35_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCB7197040.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(51321..52043)
                     /locus_tag="M2A35_000700"
     CDS             complement(51321..52043)
                     /locus_tag="M2A35_000700"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCB7197041.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(52058..53227)
                     /locus_tag="M2A35_000701"
     CDS             complement(52058..53227)
                     /locus_tag="M2A35_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCB7197042.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(53254..54870)
                     /gene="bglH"
                     /locus_tag="M2A35_000702"
     CDS             complement(53254..54870)
                     /gene="bglH"
                     /locus_tag="M2A35_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCB7197043.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFIQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNAIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(54956..56350)
                     /gene="bglB"
                     /locus_tag="M2A35_000703"
     CDS             complement(54956..56350)
                     /gene="bglB"
                     /locus_tag="M2A35_000703"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCB7197044.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMRYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(56369..58246)
                     /gene="bglF"
                     /locus_tag="M2A35_000704"
     CDS             complement(56369..58246)
                     /gene="bglF"
                     /locus_tag="M2A35_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCB7197045.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(58379..59215)
                     /gene="bglG"
                     /locus_tag="M2A35_000705"
     CDS             complement(58379..59215)
                     /gene="bglG"
                     /locus_tag="M2A35_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCB7197046.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(59501..60226)
                     /gene="phoU"
                     /locus_tag="M2A35_000706"
     CDS             complement(59501..60226)
                     /gene="phoU"
                     /locus_tag="M2A35_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCB7197047.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(60241..61014)
                     /gene="pstB"
                     /locus_tag="M2A35_000707"
     CDS             complement(60241..61014)
                     /gene="pstB"
                     /locus_tag="M2A35_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCB7197048.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(61105..61995)
                     /gene="pstA"
                     /locus_tag="M2A35_000708"
     CDS             complement(61105..61995)
                     /gene="pstA"
                     /locus_tag="M2A35_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCB7197049.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(61995..62954)
                     /gene="pstC"
                     /locus_tag="M2A35_000709"
     CDS             complement(61995..62954)
                     /gene="pstC"
                     /locus_tag="M2A35_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCB7197050.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(63040..64080)
                     /gene="pstS"
                     /locus_tag="M2A35_000710"
     CDS             complement(63040..64080)
                     /gene="pstS"
                     /locus_tag="M2A35_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCB7197051.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(64394..66223)
                     /gene="glmS"
                     /locus_tag="M2A35_000711"
     CDS             complement(64394..66223)
                     /gene="glmS"
                     /locus_tag="M2A35_000711"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCB7197052.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(66385..67755)
                     /gene="glmU"
                     /locus_tag="M2A35_000712"
     CDS             complement(66385..67755)
                     /gene="glmU"
                     /locus_tag="M2A35_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCB7197053.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(68106..68525)
                     /gene="atpC"
                     /locus_tag="M2A35_000713"
     CDS             complement(68106..68525)
                     /gene="atpC"
                     /locus_tag="M2A35_000713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCB7197054.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(68546..69928)
                     /gene="atpD"
                     /locus_tag="M2A35_000714"
     CDS             complement(68546..69928)
                     /gene="atpD"
                     /locus_tag="M2A35_000714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCB7197055.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(69955..70818)
                     /gene="atpG"
                     /locus_tag="M2A35_000715"
     CDS             complement(69955..70818)
                     /gene="atpG"
                     /locus_tag="M2A35_000715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCB7197056.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(70869..72410)
                     /gene="atpA"
                     /locus_tag="M2A35_000716"
     CDS             complement(70869..72410)
                     /gene="atpA"
                     /locus_tag="M2A35_000716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCB7197057.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(72423..72956)
                     /gene="atpH"
                     /locus_tag="M2A35_000717"
     CDS             complement(72423..72956)
                     /gene="atpH"
                     /locus_tag="M2A35_000717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCB7197058.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(72971..73441)
                     /gene="atpF"
                     /locus_tag="M2A35_000718"
     CDS             complement(72971..73441)
                     /gene="atpF"
                     /locus_tag="M2A35_000718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCB7197059.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(73503..73742)
                     /gene="atpE"
                     /locus_tag="M2A35_000719"
     CDS             complement(73503..73742)
                     /gene="atpE"
                     /locus_tag="M2A35_000719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCB7197060.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(73789..74604)
                     /gene="atpB"
                     /locus_tag="M2A35_000720"
     CDS             complement(73789..74604)
                     /gene="atpB"
                     /locus_tag="M2A35_000720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCB7197061.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(74613..74993)
                     /gene="atpI"
                     /locus_tag="M2A35_000721"
     CDS             complement(74613..74993)
                     /gene="atpI"
                     /locus_tag="M2A35_000721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCB7197062.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(75610..76233)
                     /gene="rsmG"
                     /locus_tag="M2A35_000722"
     CDS             complement(75610..76233)
                     /gene="rsmG"
                     /locus_tag="M2A35_000722"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCB7197063.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(76297..78186)
                     /gene="mnmG"
                     /locus_tag="M2A35_000723"
     CDS             complement(76297..78186)
                     /gene="mnmG"
                     /locus_tag="M2A35_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCB7197064.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(78565..79008)
                     /gene="mioC"
                     /locus_tag="M2A35_000724"
     CDS             complement(78565..79008)
                     /gene="mioC"
                     /locus_tag="M2A35_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCB7197065.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(79098..79556)
                     /gene="asnC"
                     /locus_tag="M2A35_000725"
     CDS             complement(79098..79556)
                     /gene="asnC"
                     /locus_tag="M2A35_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCB7197066.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            79708..80700
                     /gene="asnA"
                     /locus_tag="M2A35_000726"
     CDS             79708..80700
                     /gene="asnA"
                     /locus_tag="M2A35_000726"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCB7197067.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(80705..82156)
                     /gene="viaA"
                     /locus_tag="M2A35_000727"
     CDS             complement(80705..82156)
                     /gene="viaA"
                     /locus_tag="M2A35_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCB7197068.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSPLLRRWRR"
     gene            complement(82150..83646)
                     /gene="ravA"
                     /locus_tag="M2A35_000728"
     CDS             complement(82150..83646)
                     /gene="ravA"
                     /locus_tag="M2A35_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCB7197069.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEIDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            83869..85737
                     /gene="kup"
                     /locus_tag="M2A35_000729"
     CDS             83869..85737
                     /gene="kup"
                     /locus_tag="M2A35_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCB7197070.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            85904..86323
                     /gene="rbsD"
                     /locus_tag="M2A35_000730"
     CDS             85904..86323
                     /gene="rbsD"
                     /locus_tag="M2A35_000730"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCB7197071.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            86331..87836
                     /gene="rbsA"
                     /locus_tag="M2A35_000731"
     CDS             86331..87836
                     /gene="rbsA"
                     /locus_tag="M2A35_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCB7197072.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            87841..88806
                     /gene="rbsC"
                     /locus_tag="M2A35_000732"
     CDS             87841..88806
                     /gene="rbsC"
                     /locus_tag="M2A35_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCB7197073.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            88831..89721
                     /gene="rbsB"
                     /locus_tag="M2A35_000733"
     CDS             88831..89721
                     /gene="rbsB"
                     /locus_tag="M2A35_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCB7197074.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            89847..90776
                     /gene="rbsK"
                     /locus_tag="M2A35_000734"
     CDS             89847..90776
                     /gene="rbsK"
                     /locus_tag="M2A35_000734"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCB7197075.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASMNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            90780..91772
                     /gene="rbsR"
                     /locus_tag="M2A35_000735"
     CDS             90780..91772
                     /gene="rbsR"
                     /locus_tag="M2A35_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCB7197076.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(91738..93165)
                     /gene="mdtD"
                     /locus_tag="M2A35_000736"
     CDS             complement(91738..93165)
                     /gene="mdtD"
                     /locus_tag="M2A35_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCB7197077.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(93188..93880)
                     /locus_tag="M2A35_000737"
     CDS             complement(93188..93880)
                     /locus_tag="M2A35_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCB7197078.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        22892 a        24646 c        23650 g        22825 t
ORIGIN      
        1 cccctgccgc ctctttgtgg gaacactctg ccgccgccat gccgattaac atctgcgggc
       61 caaagacaaa aaaaccaatg gtgaagaagc aggttgcctg catcacgtag ctggcaaatg
      121 gcatcagcca cagcgagccg actgaaagca aaattccggc ggcgaaaatc aaattcatcg
      181 gcccacggtt gccgttaaac aatttgtccg agccccaacc ggctaccagc gcaccgataa
      241 atccgcccag ttcaaacatc gtcactgccg tattcgccgt gaccagatcg acgcccagtg
      301 tctcggacat atacaaattg ccccagtcgt tgatcgccgc ccggaccaca tagaccagca
      361 catagcaaaa cgaaagcagc cagatatacg gattcagcaa cacatatttg gtgaggatct
      421 ctttacgcgt caaccctgcc ccttcttgtt gttgagcaat ttccagcgcg tcgtgtcgcc
      481 attcaccgac cgccggtaaa cctaacgcct gcgggcgatc gcgtagccgc cagcagagaa
      541 aaatccccac gactatcgcc atacaaccag caatcatcat cccggcacgc cagccgtaat
      601 gcagcgcagc cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt
      661 tccataatgc ccaccaaccg ccgcgctcgg tacgtgaata ccaggccgtt aacagacgcg
      721 cacacaccgg tgaaccccag ccctggaaaa aggcgttcag cacccagagc acggcaaacg
      781 cccatagcga cgtcgagaag ccaaacagaa tgttgatgat gcccgtggcg ataagcccta
      841 tccccataaa ataacgggca tttgagcgat cgctgacaat gccggagaca aacttcgaca
      901 cgccgtaggt aatgtaaaac agggtcgcta acaggccgat atcgctacgg ctgagcacgc
      961 cgttagcaag gatttctggt acggcggcgt taaaactttt acgcgtgaag taaaacagcg
     1021 cgtaacccag ccagatggtc agcagaatat gccgacgcca gtagcgatag cgggcatcaa
     1081 tttcatattt atcagtcatt aatggcgcat cggcaggcgc tttcagaaac ggcaacatca
     1141 tcaaacctta gacatagcgt tgaggtagag aaacgctgac acgcgtgccg tgcagacagg
     1201 aaatgtgtaa tgtgccaccc agcgccgtta cgcgctcgcg cattccggtg aggccaaaac
     1261 cttgttgccc ggaacccggc ggcaaaccgc tgccatcgtc ttcaataacc agcatcaacc
     1321 gttcatcctg ctgccagcct tgcagcgtga ccgcgctggc atcagcatgt ttcacaatgt
     1381 tgttcagccc ttcctggcag acacgaaaca gcgtcacgcg ctggttttca cttaatgctg
     1441 attcatcgat tcgccattcg agatggctga caataccgcg cccttccagc tccatttccc
     1501 gcatcagtga gcggatggcc tgctccaggg tgagatcatc caactggcgc ggacgtaacc
     1561 gacccaacaa acggcgcacc gcgtcgtaaa cgcccagcga tagttgttcg atgagctgcc
     1621 cgctctgctt cacgctggcg ttatctgccg ccagccgctg aacaatgccc gcctgagtac
     1681 gaatagcagt gatggtctga ccgatatcat catgcagctc acgcgccaca tcacgacgca
     1741 cgctctcttc ggtttccagc aaccgttcag ccagatgctg attgcgcgcc agttcctttt
     1801 gcagcgactg gttaagttca cgcaaccgct ggatgccagc gcccagtaac aaccctgtca
     1861 gactttgcac cagcaacgag agcaataaat ccaccggatg atcgcgccag gtttgactgg
     1921 cgatcagcgc gatggcgttc atcaacgtcg cgatcagcgc cccttgccaa ccgtagtgcc
     1981 aggccagcgc gataatcggc agcgccaggc agaatggcgt aaagcgcgac agttcgtccg
     2041 gcaatcccaa ctggagccag agactaataa caaacaacag caagtaccag accagatgcc
     2101 gcccgcgcca gttgatcggt tgagaaacca gtgacgggcc gagcggcagc caggtgttat
     2161 tggcgagata gtgccagaac accagacata tcggggccag cgtcaggccg ccagttaaag
     2221 tcagcaacag cgcattccac gactctttgc cgtgccaaag ccagggcagc gactgcaaca
     2281 acgccgccgc cgttaacgcc gccccctgta acagcaaggt gcgccagtca cgctgatggc
     2341 gatagcgcga gatcagcgct acgggcagta acgtcagtaa actaccgatc atcaataacg
     2401 gaaaatgggt taaaccgacc gcctgcgtta gccagtaaat cagcagccat tccgcaccca
     2461 gcaatacggg ccagtatccg cgcgggcatt gcagcattag ccctagccgc agaccaaacg
     2521 gaaataacag caccgccata tcagggcgct caaccaggtg caggctgata ctccacaggc
     2581 aaaaccatgc ggcagagaag ataaaaaagc aggcaataac ggtaattaag cgggagaaca
     2641 acgtcttcat caccagccat caaacatgcg gcgcgccagt tctacgtcgt tactgacgcc
     2701 cagtttttcc atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca agcccagttc
     2761 ggcggcaatc tctttcaccg ccattccttg cgccagtttt tccgccacct ggcgttcacg
     2821 tttggtcagc gggtcctgac gaccggatgc cagtttaatg gcaatatccg gcgtcagata
     2881 acagccgccc gtagcaaccg tatgcaccgc cgcaatcagt tcgtcagggc tacagcgttt
     2941 ggagagaaag ccgcgcgccc ccgcgttaag cgcctgctca accagcgccg gactgtcatg
     3001 aacagagagc attattgtcg ccataccttt cggcagctgg cttagcagtt ccagaccgga
     3061 gatatcgggc atggagatat cgcaaataca cacctgcaca ccgcgccccg gcagccccgc
     3121 cagcgcctcg cgccccgaac caaactcggc aactacctgc aaatcaggtt ccagccccag
     3181 cagctgcgca aagccggagc ggacgatgag gtgatcgtct ataagggcaa cggtgatcat
     3241 ggtcttgtcc tggcgggtaa aaaaaaacgc gcttacctta acgataagcg cgatgttgtt
     3301 caagccttga gcggttactg aaaaaacacc gcaatcttgt taaacatagt cggatcggac
     3361 tgattacgct gcactttcac cacatcttcc agcttatcga tttggcttat catctgctcc
     3421 agacgctggt cgtcattgac cagtagccag atatggcttt tgtcgctgtc ctgaatcggc
     3481 agacaaagaa tgccttcaac gttaaaagcg cggcgggcaa aaaggccaca aacgtgggtc
     3541 attacgcccg gatggttgcg aacggtgagc tccagaatca cgttgtcatg agttgtgttt
     3601 tgcatggctt attcccccac catttcagta ttcgccgcac ctggcggcac catcgggtaa
     3661 actttttctt cggcatcaat gcgcacatgg atcagcgccg ggccagggcg attgatgatt
     3721 tcctgcaatg cagcctgcgg atcggcttcg ttattcaaat cacaggtttc gaggccgaat
     3781 ccggcggcaa tctgcataaa gttgattttt cccggatagg tggcggcaaa aacgccttgc
     3841 tcgtagaaca gactctgttg ctgatgcacc agccccagcg cttcgttgtt catcagaatg
     3901 attttgacat ccagctgatt ttcactggcg gtcgccatct cctgaatatt catcatcagg
     3961 ctgccgtcgc cggagaaaca caacactttg cgatccgggt tcgccagcgc cgcgccaatc
     4021 gccgcaggca ggccaaaacc catcgtgccc agcccaccgg aggtcagcca ctggcgtggg
     4081 cgattgagcg gataagcttg cgcggtccac atctgatgct gacccacatc ggtggtgata
     4141 atcgcattgt catcgacaca ggcggcaacg gcgttgatca ggccgtaatg gcttaatgga
     4201 tcgcacgctt tcgggattgg acacgggaat tcgcgctgca aatccgctac caactggtgc
     4261 cactctgcac gcggttgcgc ttccacctgc gggatcaatt gcgccagcac gtcatcaaca
     4321 tccgcctgaa tcgccacatg cggctgcttg attttaccca gctctgcgcg atcgatatcg
     4381 acatgaataa ttttggcatt cggacagaac tgctcggttt tgccaatcgc ccggtcatca
     4441 aaacgcgcac cgagcactat caacaaatcc gcctcctgca aaatatagtt ggtgctgcgc
     4501 acgccgtgca tccccagcat acccagcgac aacggatgcg cttttggcaa catgcccagc
     4561 gccattaaag tcatggtggt aggcagttgc gctttctccg ccagttcacg cacccgtgcg
     4621 ggcgcattga tcacaccgcc gcccagataa agcaccgggc gtttggcagc gttaatcatc
     4681 gccgctgcgt cacgaatgct ttcttcgcta aaggcggggg cggcggcttt ttctgccata
     4741 gcgggctgtg tttcaatctc aaaaactgcc gtttgcacat ccttaggaat gtctatccac
     4801 accgggcctg ggcggcctga ttgcgcaatg cggaaggcat cgctcatgac ctgcgggagt
     4861 tcttcgatat gtctgaccag atagttgtgt ttggtgatgg ggatagagat gccgtaggtg
     4921 tccacttcct ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc ggtgatgcaa
     4981 atcagcggga tggagtccag ccgcgcatcg gcaatggcgg tcaccaggtt agtcgcaccc
     5041 ggtccgctac aggccataca gaccgccggt ttaccgtcgg tgcgcgccat cccctgagcg
     5101 ataaatcccg cgccctgttc atgacgggcc agaatatggc ggatttgcgt gctttggctt
     5161 aaggcatcgt aaacaggcag gatagaaccg cccggaatgc ccgtcacaat cttaatgccc
     5221 tgctgttcca ggaaatgaac gataaattct gcgccggtaa agcgcttacg cgtcgatgtt
     5281 gtgcccgaac ttgccatgct ccagtccttt tcttctgggc cgactttccg ggaggtgctt
     5341 aaacgaaaaa ccccgcccgg tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt
     5401 ccctacggcg cattgccgac gaccaccacc acacgcacga cgaccactgc ggcggatagc
     5461 gcagttggta gtagttttgc gttgaacatg gaagtagtca ttggggacct tgttggtttt
     5521 gtgtttaaca atatttatac aagcacagct ttacagggga gacaatggaa aatttttcag
     5581 caagggaaaa ttgaggggct gatcacgttt tgtactgaat tgcagataac aaaaaacccc
     5641 gccggagcga ggtttcatca gccgcctgcg gctggtaacc gcaaagcaca ctgtattatg
     5701 tcaacactga aagtatacgt gttccgcgca gaacgcgcaa tttcggcacg aattttgacg
     5761 tatttagggc atagttgagt atcgatcaca gtttgcgttt tgtccaaata ttactgttta
     5821 tttatacagt aaacttctat aatatcactg tacgcaatgt gttatgcggg ggccgcatcg
     5881 ttacccggcg cactaagtcc tggctgaaac gggtggtgcc gtcagcgcct taaccccgcg
     5941 tgagcacact gtgttatgtc aacaagcaca acgtttctcc ttaagatacc gcgtgcacaa
     6001 cagctggcaa caggcagcgg aaaggtacgt cagctggcag tgctcctgaa ccacaggaga
     6061 cgcgtatgag cctggtggat atcgccattc ttatcctcaa actcattgtt gcagcactgc
     6121 aactgcttga tgctgttctg aaatacctga agtaattcag attcaagtcg caccaaaggg
     6181 gagcgggtaa ccgctcccct tttatattta gcgtgcgggt tggagttggg ggcttcttct
     6241 accagcagaa tgtttgcacc tgcgctgcct gtgcccctca ccctagccct ctccccaaag
     6301 gggcgagggg accgtcccgt gcaatgttta gctcattttc tgaaagggaa cggtggtttc
     6361 cccctcgccc ctttggggag agggttacag ttcgcacgta atccctcccg gcaacattta
     6421 ttgccgcttt tgtttacata ttctgccgct aaacaattct ccattcctgg cgtatatctg
     6481 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
     6541 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
     6601 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
     6661 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
     6721 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat ttccgaccgc
     6781 gtgggccgcc gaccggtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
     6841 gcggtcacga cctccagttt gacggtgttg attgccgcca gcgcgatgca ggggatgggc
     6901 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgag atttatatga acggacacag
     6961 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
     7021 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
     7081 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
     7141 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
     7201 aactgttatt tgctgatgct gattggcggt ctggccggga ttgccgcctt tgaagcctgc
     7261 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
     7321 attctgccga ttccggcagc gttttttggc gcatggtttg ccggacgtcc caataaacgc
     7381 ttctccacgt taatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
     7441 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
     7501 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
     7561 ttcctggcgg gcacggctgg cgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
     7621 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
     7681 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
     7741 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
     7801 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
     7861 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg gcgagcgtgc gggcacaggc
     7921 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
     7981 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
     8041 actcgaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
     8101 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
     8161 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagtt
     8221 tatacctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
     8281 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
     8341 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
     8401 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgagaatgcc
     8461 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
     8521 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
     8581 tcctgggtcg gcaatgcgtc tggctttgcg gctatccggc atacaacacc agtcccataa
     8641 cgatcaccgc cacgaccatc aatattaagc taatgattaa taagctgttg gtgtagggca
     8701 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacatcg
     8761 ctaacccacc ggaaaataga cacaacaaca acgccagcag ttcgcgaatg acgggcgtgg
     8821 cgaaatctgg cgcaagttgg tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
     8881 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
     8941 cgaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
     9001 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc ccctacaagg
     9061 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
     9121 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
     9181 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
     9241 aataaaaggc gaaaaaatta ctaccgtgga gaaaaaagag gacgtagatg tcgataaaat
     9301 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
     9361 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggcg gcgggacgct
     9421 ggctttccat accttgcttt ttggtatcgg catcgtgtgc agcattttgc tgatattcct
     9481 tatcttttcc ttcctgactg gcggttcact ggtttaagca gcctgaataa tgcccgccgg
     9541 gtgttcatca gcgggcatta tcgtcagaac ttctgttttt tctcttccac tttcactcct
     9601 tgcgtcggta ggccggtgtc atagtcacgt accaccggcg aatagccatc ttgtgggcgt
     9661 ggacgaaatg cccccatcca gcgcggcagt gcatctttac accacggacg caggctccat
     9721 tggtaactgc ggaacggatc gcgaattttg tccatgtaat ccaataaggc gtcatgcatt
     9781 ttgctgcgaa cgtctgcaaa acggatatca tcgatcaggt tatgcatttc atttgggtca
     9841 ttacggcgat cgtaaagttc atcactggtg aagaggttga gtaccagttt aaagtcatcc
     9901 gtcacccagc aacgcaccgg aataaaaccg ccaaagctgt catgctcaat ctcgtagcgg
     9961 ttaaattcca ccattacacc gcgtggctct ttcacggcaa ggatattttc ccccggcaga
    10021 atctctggtt tttcaatatc tgccagcgcc atcattgtcg gcagtaaatc gatatgactg
    10081 actggcgtat cgacctgtcg ccgctcccct tgcggcgaac ggatgatcag cggaatacgg
    10141 gtgatgtcgt catacatcgc cgctccttta ctgatcagct tatgtgcgcc catcatttcg
    10201 ccgtgatcgg aagtataaat gacccacgta ttttcacgtt gctctggcgt taaggcattg
    10261 atgacccgtc cgatttggtc atcaacaaag tcattacagg caaaatagag cggatggtga
    10321 taaagcccgt catcaccgac tggcgatggc atcgcctgcg cccataagcg gtgatgttcc
    10381 ggtttgttcg ccaggtcatc ctgagctttc tcgcccagat cgtagtaaaa atcagcgtat
    10441 ttctctaaat actccaccgg acaggtgaac gggtgatgcg gctcatcata cgaaaccacc
    10501 atcaggaagg gttcctcggc gcgcgcgggc tgttgcagaa aatctaccgc ccgattgctg
    10561 atgcggtgcg cccaggtgaa ggtttcgtcg atatggttcg cctgtaaatc ctcaacgctg
    10621 tttaggccat tgcgccacag gctgatctct ttttccgtca gttcgctaag gtagttcgcc
    10681 ccatcgaacc agtaatcagc gtcccactcc ggcggacact cgccagtgcc gaaatagtca
    10741 tgaccgtcga gatgccattt gccgatgtaa caggtgtgat agccggcatc cttaaagtag
    10801 cgccccatag tggagatgtt tttgcctggc gcgacgttgt tggtccacgg gccggactgg
    10861 ttagcgtaga taccggtaaa tagtccggcg cgtgcaggcg tacaaaccgg tgaacaggtg
    10921 taggcggaat taaagcgaat accttccgcc gccagactat caatattttg cgtattcagc
    10981 ggtttaccgc tatagcaacc gaccatattg gtggcctggg tatcggtcat gatgaacaga
    11041 aaattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    11101 gacgatgccg ccagctgtca aaaatcaggt agacaatcac tacggcggca atgaaataac
    11161 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg gcatagacgc
    11221 caatcatcgc gaacaggatg ccaattgacg cgatcttgac gtttctccac ggtttcatgt
    11281 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    11341 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    11401 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    11461 ttgccgccag cgccgggatg cgcgggaaga aaaagcccat aatgatgatg gtaaccagcg
    11521 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttgg
    11581 cgatccacgg cgcgaccagc accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    11641 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    11701 tgaataacgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata
    11761 acaccgcgcc gaagaaaccc accattggca ctggcagaac gttattgacc agcgtcgggt
    11821 aggccatgtc ggctttcggt aaatcctgat acagatgaaa cgcaatcaac cctggcagca
    11881 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    11941 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    12001 aggtgttcac cagaatcaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    12061 caccgattga gtttaatttc tcggcgtgaa cggtggtgag ttgctcaatg ccctgcataa
    12121 agctgccctt gcccatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    12181 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    12241 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    12301 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    12361 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    12421 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    12481 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    12541 ccatcaccga catgccgctt ttgtaggcct gcccggaaag gccgaccagt tgttccgtgg
    12601 aaaggttggt tagcattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    12661 gaaaatagcc ttgttgcgat ccggtatcta ttttgcggac cttccaccag gtgatcaccg
    12721 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    12781 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    12841 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    12901 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    12961 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    13021 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    13081 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    13141 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aacactgcat ggcaactgta
    13201 ttatattctt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    13261 agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt cagcaaagcg
    13321 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    13381 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    13441 ggatttgtct tatgtacgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    13501 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    13561 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    13621 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    13681 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    13741 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    13801 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    13861 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    13921 taatcgccta ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    13981 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    14041 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    14101 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    14161 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcca
    14221 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    14281 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    14341 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    14401 aaaatcaacg tcgctgaatg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    14461 cgctttctct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcaccatc
    14521 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    14581 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    14641 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    14701 agctgttcac gcagctggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    14761 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    14821 tcatgcagtg caatacgtaa acgcgtcgcg caattgttaa tgctggagat attgccgacc
    14881 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgccgcg
    14941 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    15001 tcacgtcccg gcgtgcacat gttgaactgc agaatcagtg tgcggaaaac cacgaagtac
    15061 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    15121 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    15181 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    15241 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    15301 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    15361 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    15421 agggcaaaac cgccttccgg gaacaacgat ttcagcggct cggcgctcaa actgaactct
    15481 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    15541 aactgtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    15601 taaacccaga ccccaagtgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    15661 atttgtactt ttggccagcc gagcaacgtc agccaggcac aggggatcat caccaggaag
    15721 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    15781 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    15841 ctggtatcga gggttttaat cccagccatc attgtcagac cgctacctgc caccgcgtcc
    15901 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    15961 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    16021 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    16081 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    16141 ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga
    16201 aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc
    16261 acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg caggcctgga
    16321 gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt
    16381 tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag
    16441 cactggtaca cagcatcgaa cacatcttta aaaaaaagat gttttttcaa tcgattaagc
    16501 agaacttgtg ggcacattac ccgggcttgc aggcaaaaaa gagatctaga gatgatctac
    16561 aaaagcattg cggagcggtt aagaattcga cttaattacg cggatttcac gctaaacagc
    16621 cttcttcccg gtgaaaaaaa gttggcggaa gaatttgcag tatcccggat gaccatccgt
    16681 aaagcgattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg cagcggcact
    16741 tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact ggtggaggtg
    16801 ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat catgcctgcg
    16861 cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta cttctcccgt
    16921 cgcgttcgtt ttgtggaagg gaaaccgctg atgctggaag acagctatat gccggttaaa
    16981 ctgttccgta atctttcgct gcaacatctg gaagggtcga agtttgaata tattggacaa
    17041 gagtgcggga ttttgattgg cggtaattat gaaagcctga cgccggtgct cgccgataga
    17101 ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat cacctcgttg
    17161 tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa tgccagcgaa
    17221 taccaggtgg agtaccattt acggcgactc cacccggaaa agagttaacc gatactccag
    17281 aagagcaccg ccagtaattg gggggtgata atgcgcagaa acatcactaa cggatagaca
    17341 gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc aaacgccagc
    17401 gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta attcattttg
    17461 gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat cagggcacca
    17521 taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc cccaccagat
    17581 ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc gaggttggca
    17641 cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag gatcaacgcc
    17701 ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaaccc aggcacaaag
    17761 acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg cagcatctga
    17821 acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc aattgcggac
    17881 ggacgcccca ccagattgag gatatcgccg aactgcaggc tgatatcgcc actggcgacc
    17941 agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc tttaaagtgc
    18001 aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg ctcgacgcgc
    18061 aaatcagtgc ctttcgtgga cagcgaagta tcgacctcct gaccaatcac cagttgcgca
    18121 ttatgtaaat ccgctggctg acccaccaga tgcagcaaat cgcccagttg aataatggta
    18181 tctggcgaag gaacttttag ggtttcttcg cgtttcagac gcgagcagat aattttgtcg
    18241 ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt ctcaacgcga
    18301 atattgatag tcttgatcag cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct
    18361 tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa caaaatcccg
    18421 caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac catttccatt
    18481 ggtgtaccca ggtcgcgcaa aatctgctgc cctgccccca gcgctggcgt attggtaacc
    18541 gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt atgcaggatg
    18601 gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag gcgtaatccg
    18661 gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatagt ataaacgaac
    18721 aggatcaggc caaattcctg aataacatgc agcatatcgc tacttaatgt catccccgcc
    18781 tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat acctaatcct
    18841 atgccgcgaa atttgacatt gccgataaac aaaccgacga ctgccaccaa agccagaata
    18901 ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat ttttaattgc
    18961 tacggattat ggctgaatcg gtgaagcaga tttgccttat agcgcacatt atcacgggaa
    19021 agaacgtgcc gaaatatctt aaacagtcga aatttaacgc attgttgagt aagtaagaca
    19081 aaaaaagccc caccagaatg gcggggcaaa gagaatagct agttagctat ttaacgcggg
    19141 acgttcgctg atagcgatac gctgcgctgc gatgggttca ggctcattac gaattaaatc
    19201 aatatgcagt aaaccgttta cgaaggttgc gccagagact tccatatttt cagccagcgt
    19261 aaagctcagg ctaaatggct gattcataag cccttgatgc agccattttt tctcttcttt
    19321 tggctgctcc ggcgtgcctt ttacgctcag gcgcgtacct tccagttgaa tctctaaatc
    19381 ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag tggttatcgt cgcttttctc
    19441 aatgttgtac ggcgggaagc tctggctttc accggcgttt tgcagtgcgt tggccagttt
    19501 gtcaaaaccg atccattgac gcatcagtgg ggataaatcg aagttacgca tagtcatttc
    19561 tccttctaag aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc tccctgacgg
    19621 cgagcatgga gatgtcaggc cgcgccaggc ggccttaggg aattagttga tttcgatacg
    19681 gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg atatacagca aaccatttac
    19741 caggttagca ccacgaacat gaatgttctc agctaactgg aatttgcgtt caaagttgcg
    19801 ttcagcgatg ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt gagcaccttt
    19861 caccaccagc agattatcct gggcggtaat ttccagttcg ctctcagcaa aaccagccac
    19921 agcgatagca atgcggtaat ggttttcgtc taccagttca acgttatacg gagggtagcc
    19981 gccattactc tggctctggt tgttttctaa gtggttaaac aaacggtcaa atccaatagc
    20041 agaacggtaa agcggggata aatcaaagtt acgcataatc aatagctcct gaaatcagcg
    20101 agaatgtaag accttccaca atggacaggt caggtagcca gaacacccat caggcgcgtc
    20161 ctcatcggct acgatgtaaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga
    20221 cttttttttg cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag
    20281 tgcgatagcc gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata
    20341 actcatcagg ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt
    20401 tagcgttcat gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc
    20461 ataccggagg gaaagaagga acgtatcccg gcacccgcgc cagcgcaacc atgattggcg
    20521 acgatgaaac caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg
    20581 tgatggacac gcttttactg ccgtgggatg tgttccgcaa agacagttcg gtgcgctcac
    20641 gcgttgaaaa aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa
    20701 tgcctgataa ctgatttagc gatcagtttc ggtgatccac agctggccac cttccatcca
    20761 cgccagccat tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg
    20821 gttttcggtt aaatactcaa cctcgccgct ttgcgcatgg gcacaagcaa ttcgattatc
    20881 cagcacaaag cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt
    20941 tttgttatgg gttaactggc gcggctcgcc gccctgtggc gagataagcc acagttgcac
    21001 aatgccgtta tcatcacgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac
    21061 ccagtggcgc gggacgttga ctaaccccgg ataagcccga tggtgggtaa aggttaaacg
    21121 tcgctgcacg acgccgcgcg gtggcgcggg cagggtggtt tccgttccac ttaacggcgc
    21181 atcacctgcc gctttccagc cagcttcatc ttgcggtaac tcaacgataa acagctccgg
    21241 cactttctcg ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac
    21301 ccatccttct tcataagcac gattgatttc atcgctgcca ggctgcggcg tgggcgtggt
    21361 tttactcacc agcacgcacc agtggctacc gctgtattca cgcggatgct gcttttgtac
    21421 gttgaccggg ccaaacggcg cagcaacacc gacgtttcgc aaatccagcg ccggatcgag
    21481 ttcatgcatt acatggtcgt tataggtaaa gctcaccctt tcaccgttcg ggctaaagac
    21541 atgcacatgg ctgccgccgc gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc
    21601 atcgagattg ctcaccttgc cgccttcagc aatgactccg cgtcgatgat ggaaatcgta
    21661 atgccatgtt tcatcaggat tttccgggcc atggatgaaa acatatttct ctgacttcgg
    21721 atgaacggtc accacgccga cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc
    21781 gccggtatgg atattcacac gctcaatggt ctcgccggta aacgacgccc cagaaggacg
    21841 cacgtcaaat accagccact ggctgtcggg cgtccaggta ttggtattgg tgagcaggtg
    21901 attacgggga gcaaaggtta tctgtttcat ggcgcggtcc tgatacaaaa cgggtcccac
    21961 catcatactt cacaaggact tcactttcag acaatagtct tttgccatcg tgatgaatgt
    22021 gagtgaaaag atggaacatt tcgacgtggc gattattggc ctcggcccgg caggatcggc
    22081 gttggcgcga aaattagcag gcaaaatgca ggtgatcgcg ctggataaaa agcaccagtg
    22141 tggtactgaa ggtttcagca aaccttgcgg cggtctgctg gcaccggacg cgcagcgatc
    22201 ttttattcgc gatggactga cgcttcctgt cgatgtgatc gccaatccac agattttcag
    22261 cgtcaaaact gtcgacgtcg ccgcatcgct cacgcgtaac taccagcgaa gctatatcaa
    22321 tattaatcgc catgctttcg acttgtggat gaaatcgctg atccccacca gcgttgaggt
    22381 ttatcacgat agcctgtgcc gaaaaatctg gcgtgaggat gataaatggc atgtcatttt
    22441 tcgtgcagac ggctgggagc aacatattac cgcccgctat ctggtcggtg ccgatggcgc
    22501 aaactcgatg gtgcggcgac atctctaccc ggatcatcaa atccgtaaat atgtcgctat
    22561 ccagcagtgg ttcgcggaga aacatccggt gccgttctac tcctgcatct ttgataattc
    22621 gataactaac tgttattcat ggagtatcag caaagacggt tattttatct ttggcggtgc
    22681 ttatccaatg aaagacggtc agacgcgttt cacgacgctg aaagagaaaa tgagcgcctt
    22741 tcagttccag tttggtaagg cggtgaaaag cgaaaaatgc acggtgctgt ttccctcacg
    22801 ctggcaggat tttgtctgcg gtaaggacaa cgcctttctg attggcgaag cggcaggatt
    22861 tatcagcgcc agttcgctgg aggggattag ctatgcgctg gatagcgcag acattctgcg
    22921 tgcggtgtta ctgaagcagc cagagaagct caataccgct tactggcgcg ccacccgcaa
    22981 actgcgttta aaactcttcg gcaagatagt aaaaagccga tgcctgaccg caccggcttt
    23041 aagaaagtgg attatgcgca gtggtatggc gcatattcca cagttgaaag attagccaac
    23101 gcgcttcaca tcgcccacca gcaggatgta agagagcgcg ccaatcaacg cgacggcgga
    23161 gatataaacc agtgcaggtg cgaaaccgta accctgcgcc aggtacccca ccaccagcgg
    23221 aacggtgatg ccgcccagac caccggcgaa gttaaacacg ccgccggtta aaccaatcag
    23281 gcgcatcggt gccagagaag agaccagcga ccaggtaatc gaagcaaaac cgttgccgaa
    23341 gaatgccagc gccatcaggc acataatcat catcggatcg ttggtgtagt tagcgcccat
    23401 aatgcaggtg gagatcagca agccgcagat aatcggcgtt ttacgcgcaa agcccagtga
    23461 aaagccctta cgtaccagca gatccgcgac ccagccagag agcaggacgc cgacaaacgc
    23521 cgcgaggaat ggcaccgtgg tcataaagcc tgctttcagc gccgtgattc ctttttcctg
    23581 ggttaaatag ttcgggaacc aggttaagaa aaaccacagt gtagaagcca ccgcaaattg
    23641 cccaagataa acgccgatca gtttacgatg gaacaccagt ttccagtctt tggctgttaa
    23701 cggctgacgc gcctctttct tcaccggcgc atcaccatcc accagaccgc cgccatcacg
    23761 aatgtaatcc agttcagctt tgctgatacc tttggtcagg cgcggcggct gataaacctt
    23821 aaaccaaatc agcgaccaga taatgccgat accaccagtg acaatgaaca cccagtgcca
    23881 gctcaacatc tcctgaatcc agatcagcag cggagtcaaa aacgccagac cgacaaactg
    23941 accagacgta taaaaaccaa cggcggaagc gcgttcatgt tccgggaacc agctggtcac
    24001 catccggtta ttggtcggga aagcgggcgc ttcgaaaata ccggttatcg cacgcagacc
    24061 aattaatgac attaatcccg tggcaaagcc ctggaataaa gtcgccactg accaaccaaa
    24121 tatcgcaata aaataagtca cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt
    24181 ataaagccag gcgaaggccg aaaatacata gcccatttgc gctttggtaa taccgaactc
    24241 ttcctgaata tgggcggaag ccacggccag gttagcgcgg tcaacataac aaatgaccac
    24301 cgtaataaag atcatcacca gcgtcagata acgccgacgc cccggttttg ctgcattaac
    24361 gggaatatcc atagcgagct ttctccagat tttgggcata gcgaagccgc tcaccatgcc
    24421 ctgtaattta cagagggtta tatttttgta ttgctgtttt agtgcccgat gaggggctta
    24481 cgtggcagga attaccactc tgctacgctg ttatcttcat gacgccagag cggattacgc
    24541 cagtccgggg catttttact gaactcaata actttagctt cgtcgatttc cacgcctaag
    24601 cccggtttcg ttagcggttt aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc
    24661 acaaagtcga gtaactccgc gcctttgttg taatgaatgc ccatactttg ttcctgaagt
    24721 acggcgttat aggaaacaaa gtcgatatgc aggcaagccg ccagtgcaat cggtccgagc
    24781 ggacagtgcg gcgcaagggt cacatcatag gcttctgcca ttccggcaat tttgtagcat
    24841 tcggtaatac cgcctgcatg ggagaggtcc ggttgcagaa tcgaaatacc acctgcctcc
    24901 agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac ccgccgccag tggaatatgc
    24961 gtttgtgccg ccagtttcgg gtagtattcg gcttgttccg ccagcaccgg ctcttcaata
    25021 aacagcgggc gatacggctc cagttcttta atcagcactt tcgccattgg cgcgctgacg
    25081 cgaccgtgga aatcaagacc aaactcaatc tgattgccaa aagcttcacg aatttgtgcc
    25141 acggtgttga ctgccgcatc taccgcgcgg gagttatcaa ttagccccag ttcttcacaa
    25201 ccgttcagtt taaaggtatc gaagccgatt tcgcgcaggg ttttaatgcc gtcgataaca
    25261 tccgccggac gatcgccgcc gacccaactg taggctttaa ttttgtcgcg aaccaggccg
    25321 cccatcagtt gccagaccgg cgcattcagc actttacctt tgatatccca taacgcctgg
    25381 tcgatcccgg cgatggcgct catcaggatt ggaccgccac gataaaatcc ggcgcgatac
    25441 atcacttgcc ataagtcatt gatgcgcgat ggatcctgac caatcaaata gtcacccagc
    25501 tcgtgaactg ccgcttccac cgtgcgggcg cggccttcaa tcacgggctc gccccaaccg
    25561 accacgcctt catcggtttc aattttcagg aacatccagc ggggaggtaa acgatacgtg
    25621 gtaattttgg tgattttcat tgcactgcct ctcgatacgc cttaacaaat gctgctgcct
    25681 gctgcgcggt gcgttctacg gactgcccgg cgcgatagag atcgctgcct aagcccgccc
    25741 cagcacaacc tgcgtctatc cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa
    25801 agactgcgat gtccgatggc aataccgctt ttaacgcttt gatgtattgc ggaccaaaag
    25861 ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg
    25921 tcgccgtcgc gcagccgggg cagacggtca tgccgtagcc caccgcacgg cggatcactt
    25981 cactatggat attgggcgta acgatgagct gacagcccat cctggcgagc gcatcgacct
    26041 gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga
    26101 tggcgggaat gctttgctcc cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc
    26161 cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc gggcttaatg ccgcgcaaaa
    26221 tggcgatcag cggaagttta gtttgccact gcatgagcga tgctccttat accagcctga
    26281 aatgccgtgt cgcccgccac cgccgtcacg tcgcaaccca tcgcctgaaa ggcttgctgg
    26341 tagcgcgcgg tcagcgatgt tccggcgaca agggtgatgg cgtgttgatg ggtcacatag
    26401 tcgcgcatac tggcaacctc tgcgccaatc aacaaaccag agagaaattc gctgacctgt
    26461 tcgcggggaa gtgttcccag cacatgcgag gcgcgaactt caaaaagctg cggcaatatg
    26521 gcgggcgcat taaggccgcg ctcaaggcca gccgcgaagg catcggcaga gttttcctgc
    26581 ggcggcaaac ctgcgccaat caatgagtga tttaacagta aatgatgtaa ttcaccggtc
    26641 atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct gcacccattt gcaatgcgta
    26701 ccgggcataa cataaagaga ggaaggagcc agagctcgcg cgccgatcaa ttgtgtttct
    26761 tcgccgcgca tcacattgtg gttatcgtca tgagagacgc ataatccggg aataatccag
    26821 atattgtcgc caacagacgt taattgttcg ccaatagacg aaaaacgggc aggaacagat
    26881 aaatacggag caactttcca gccgacattg ctgccgacca ttcctgccat caccactggc
    26941 gtattttctt cacgccagtc ggtcgtgact tctgctaaca ccgcagccgg agattttccg
    27001 ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca ggcagtggtc gccctgataa
    27061 agccaggcgc gcagattggt cgatccccag tcaattgcga tgtagcgagc tgtcatgtga
    27121 tttcctttaa ccttcgtgtc gagctggcga tcatggtaag cgccgcctgc tctgccgcat
    27181 cgccgtcctg atgccgtatt gcatcgaaca gcgccttatg ttcctggagc gtttgcggca
    27241 tgttggcctc atcgcccatc caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg
    27301 caatgctaag ttgctgtaac accgggttat gcaccgactg cagcaccgcc tcgtggtagc
    27361 gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg
    27421 ccgattcaat ctgcgccaga tcgctggaag tcgcgcgctc tgctgcccaa cgggcaatcg
    27481 ccggttccac cagatttcgc acttcgctca tggcactgat aagccgtggg tcgtagtcgt
    27541 tttccaacac ccattgcagt acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca
    27601 caaacgcccc gcgataacgt ttcatttcaa tcagccgctt cgccatcagc gaacggaaca
    27661 cctcacggat gatgttgcgc gaggttgcaa actcttcaca gagctccgcc tcagccggaa
    27721 gtggcgagcc aggtacgtat ttgccgtgaa cgatctgttt acccagcgta atgacaatgc
    27781 gatcggtttt attgagagtc atggagagtc cttgtgctct gtatgttctt ctctacttta
    27841 ccccgatcga tgcataacgc ggcaactttg tagtaccagc gtgatgacgt tcgcgtttgc
    27901 cgtgtgcgta atgtagtaca aacttatatt gttgtactac aatttagatc acaaaaagaa
    27961 caatgcataa aaaaatgaca tgcgtcgggc agaaatctga aaagggatat caggcgctaa
    28021 acaggaggga aagaagagta tgctttcaac ggcttagcta ctcgtttaaa ggattaatca
    28081 tgaagttgaa ttttaaggga ttttttaagg ctgccggttt attcccactg gcgctgatgc
    28141 tttcaggctg tatctcgtat gctctggttt cccataccgc aaagggtagt tcaggaaagt
    28201 atcaatcgca gtcagacacc atcactgggc tatcgcaggc aaaagatagt aatggaacaa
    28261 aaggctatgt ttttgtaggg gaatcgctgg attaccttat cactgatggt gccgatgaca
    28321 tcgttaagat gctcaatgat ccagcactta accggcacaa tattcaggtt gccgatgacg
    28381 caagatttgt tttaaatgcg gggaaaaaga aatttaccgg cacaatatcg ctttactact
    28441 actggaataa cgaagaagaa aaggcactgg caacgcatta tggttttgcc tgtggtgttc
    28501 aacactgtac caggtcactg gaaaacctaa aaggcacaat ccatgagaaa aataaaaaca
    28561 tggattactc aaaggtgatg gcgttctacc atccatttaa agtgcgattt tatgaatact
    28621 attcacccag aggcattccg gatggtgttt ccgcagcatt actgccagtg actgttacgc
    28681 tggacatcat tactgcaccg ctgcaatttc tggttgtata tgcagtaaac caataatcag
    28741 taagcgggca aacgcgttta tgctgtttgc ccgtccactg attaattcag cacatacttc
    28801 tcaatagcaa acgccacgcc atcttcaagg ttggatttgg tgacaaagtt cgccacttct
    28861 ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc ctgcatattc aatcattgcg
    28921 atatcgtttt cctgatcgcc aatcgccatg atttcttccg gtttaatacc taacacgtcg
    28981 gccagtgatt tcacccccgt acctttgtta acgcgtttat cgaggatttc gaggaagtac
    29041 ggcgcacttt tcagcacggt atatttctct ttcacttcct gcggaatacg cgcgatagcc
    29101 tggtcgagga tggcgggttc atcaatcatc atcactttca ggaactgggt attggggtcc
    29161 attttctccg cttcgcagaa caccagcgga atggtggcaa cgaaggattc atgcaccgtg
    29221 tagtagctga tatcacggtt ggcggtgtac agcgtggtgc ggtccagggc gtggaaatga
    29281 gaaccgactt cgcgagagag tttttccagg aaacgatagt cgtcatagct gagagcagtt
    29341 tgcgccacgg tgctaccatc agcggccttc tgtaccagcg cgccgttata agtaatgcag
    29401 tagtcgcccg gctgttccat atgcagctct ttcaggtagt tgtgcacacc tgcatacggg
    29461 cgacccgtcg ttagcacgac attcacgcca cgggcgcgag ctgcggcaat cgcattttta
    29521 acggcgggtg aaatggtgtg atcgggcagc agaagggtgc catccatatc gatagcaatg
    29581 agtttgatag ccatgagttc cccaggtaga ttagttcctg acccatgcta acgcgattcc
    29641 gctcaaaaat cagtacaaca cccgagggaa aagggggatg caacgcgcgt tgcgtgctcc
    29701 ccatttgctt agcggaagag tttccctttc agcagttcca tgcctgcgga aagcagatcg
    29761 ttattggctt gtggagacac ttcaccttgc ggtgagagcg catcaataat cttcggcaat
    29821 tgttctgcca gtaaactgga agctgtactg gtatccacgc caagtttttg cccgagatcg
    29881 gacaccgcat ttgtgccgag cgccgattcc agttgctcgc cactaaccga ttgattgccc
    29941 tgttgattac tcagccaggt tgagagaatg gcccctaagc cgccactttg cagtttttcc
    30001 agcagcacct gaatgccgcc ctgctcctca acccaactta aaatcgcctg atatttcccc
    30061 gcatcgcctt tcagaaaggc accgacaact tcatcaaaaa gccccatgat aatcacctgt
    30121 aaagcgttac gtgttgaccc aaaaagtata gatctgcggg atgataattg cggattgcag
    30181 aaataaaaag ggcggagatg atctccgccc ttttcttata gcttcttgcc agatgcggcg
    30241 ctaacgcctt acccggccta caaaatcatg aaaattcaat acattgcaag attctcgtag
    30301 gcctgataag cgtagcgcat caggcacgct cgcatggtta acgccattaa atatcgatat
    30361 tcgccgcttt cagggcgttc tcttcaataa acgcacggcg cggttcaacg gcgtcgccca
    30421 tcagcgtggt gaacagctgg tcagcagcaa tcgcatcctt aacggtaacg cgcagcatac
    30481 gacgactttc cgggtccatg gtggtttccc acagctgttc cgggttcatc tcgcccagac
    30541 ctttataacg ctggatggag aggccgcgac gggactcttt caccagccag tccagcgcct
    30601 gctcgaagct ggctaccggc tgacgacgct cgccacgttc gataaacgca tcttcttcca
    30661 gcaagccacg cagtttctca cccagcgtgc agatacgacg atattcgcca ccggtgataa
    30721 actcgtgatc cagcggatag tcagtatcca caccgtgggt acgcacgcga acaatcggct
    30781 cgaacaggtt ttgctcggca ttggtgtgga catcaaactt ccactggctg ccgtgctgtt
    30841 ctttgtcgtt cagttcgctg accagcgcgt tcacccagcg ggtaacggtc tgctcatcag
    30901 agaggtcggc ttccgtcagc gtcggctgat agataagctc tttcagcatt gctttcggat
    30961 aacgacgctc catgcggttg atcattttct gcgtcgcgtt gtactcagac accagtttct
    31021 ctaacgcttc gccagccaat gccggtgcac tggcgttggt gtgcagcgtt gcgccatcca
    31081 gcgcgatgga gatttggtac tgatccatcg cttcgtcgtc tttaatatac tgttcctgct
    31141 tgcctttctt cactttgtac agcggcggct gagcgatgta gacgtgaccg cgttcaacga
    31201 tttccggcat ctgacgatag aagaaggtca acagcagcgt acgaatgtgc gagccgtcga
    31261 cgtccgcatc ggtcatgatg atgatgctgt gataacgcag tttgtccggg ttgtactcgt
    31321 cacgaccgat accacagcca agcgcggtga taagcgtcgc cacttcctga gaagagagca
    31381 tcttatcgaa gcgcgctttc tcaacgttga ggattttacc cttcagcggc agaatcgcct
    31441 ggttcttgcg gttacgcccc tgcttcgcag agccgcccgc ggagtcccct tccaccaggt
    31501 acagttcgga aagcgccgga tcgcgttcct ggcagtctgc cagtttgccc ggcaggcccg
    31561 ctaagtcgag cgcaccttta cggcgggtca tttcacgcgc gcgacgcgcc gcttcacggg
    31621 cacgggcagc atcgataatt ttgccaacca cgattttcgc gtcggttggg ttttccagca
    31681 ggtattctgc cagcagttcg ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa
    31741 ccagtttgtc tttggtctgg gaggagaatt tcgggtccgg cactttcacg gaaacgaccg
    31801 caatcaggcc ttcacgcgca tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc
    31861 cttctttgtc catgtaggca ttcagggtac gggtcatcgc cgcacggaag cctgccaggt
    31921 gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca gtagatgttt tcctggaagc
    31981 catcgttcca ctgcaacgcc acttcgacgc caataccgtc tttttcggtg gagaagtaga
    32041 agatattcgg gtggatcggc gttttgttct tgttcagata ttcaacgaac gccttgatgc
    32101 caccttcata gtggaagtgg tcttctttgc cgtcgcgctt gtcgcgcaga cgaatggaaa
    32161 cgccggagtt gaggaacgac aactcacgca gacgtttcgc cagaatttca tattcgaact
    32221 cggtcacatt ggtgaaggtt tcgaggctgg gccagaaacg caccatggtg ccggtttttt
    32281 cagtctcgcc ggtaaccgcc agcggggcct gcggtacacc gtgttcgtag atctgacggt
    32341 gaattttacc ctcgcgctgg ataaccagct ccagtttttg cgacagggcg tttactaccg
    32401 aaacaccaac gccgtgcaga ccgccggaca ctttatagga gttatcgtca aatttaccgc
    32461 ctgcgtgcag aacggtcatg atcacttccg ccgccgatac gccctcttcc gggtgaatac
    32521 cggtcggaat gccgcgcccg tcatcctgta cagagacaga gttatcggcg tgaatggtga
    32581 cgataatttc tttacagtga cccgcgagcg cttcgtcgat agcgttatct accacctcga
    32641 ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct
    32701 tacgcaccgc atccagccct ttcaggactt tgatactgga ggagtcataa gaattcgaca
    32761 tcaacgtttc tcgctcattt atacttgggt taatccgtta ttttaccctt ttccacggta
    32821 aacatcttcg aattttcgtc cgacatgtct ataacgtgtt cagcactgat cgcgctgaca
    32881 aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca acccgcgacg ctcatcatca
    32941 agctcagagg caaaatcatc tatcaggtag agacaccgcc gcccgctttc acgggtgagg
    33001 aactctcctt gcgccagacg taaggcgcac atcaacagct taagctgccc acgcgataag
    33061 gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt ccgctttgtg cgggccatgc
    33121 gcggtgtagg ttagctggcg atcgcgttca aaattacgtt ccagtacctc agcatattct
    33181 gtctctttct cccagccgcg ctggaaagag aaagtcagag aaaactcagg gagaaattgc
    33241 ttacaggtat cagccatatc ggccgcgata ccggcgctgt actgggcgcg ccaggtgctg
    33301 atttgctccg ccagcgggat cagttcttta tcccacgggc gtagctgttc gtaacgtgtc
    33361 acctggcgca gcgccgcatt gcgctgcttg agcaatcgct tgagattgct ccaggcggtg
    33421 aaaaatccgg gttcgttgtg aaagcatccc cagtcgagga atgctcttct gtatttgggg
    33481 ccgccgttga gtaaagtaaa cccttctggc gttatcagct gcattggcat caggtgcgcc
    33541 agttccgcga ctttatgccc gtcagtaccg tcgatgcgga ctttgctgtc gccctgtttg
    33601 tccttggtta agccaatcgc cgtctcgcgc tcttcgccct gtaatcgccc atggagaaca
    33661 aatgcctcct gctcatggcg aatgacgcga ccaatctgca aactgcgaaa cgcccgacca
    33721 tggccgagcg tatagatggc ttccagcacg ctggttttgc cactgccgtt ggcacctacc
    33781 agaaagttaa agccgggaga taaagcgaga tccgcggttt caatgttgcg gaaatcgcgg
    33841 atcaacaagc gggtgaggga cattacagtc tcattggcat gacaacatag gcagccgact
    33901 gtgatgcggc atcttcaatc tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt
    33961 tttcgcattt cagcgcgttc agaacatcca gcacatagct gacgttgaag ccgatttcca
    34021 tctccgcacc gctataggta acgtcgagga tctcttccgc ttcttcctgt tccgggttgt
    34081 tggcggtgat tttcagctgg ttttcgctga catacaggcg cacgccgcgg aatttctcgt
    34141 tagagagaat tgccgcacgg gcaaacgcct gcttgagcag atcgcagcca gcttccagat
    34201 gtttgtccgg attcttcggc agaacgcggc ggtaatccgg gaagcgacca tccaccagtt
    34261 tggaggtgaa gataaagtcg ccaacgtggg cgcgaatatt gttgctgcca atctgcacgc
    34321 gcagcggatt gtcgccgccg tcgagcatac gcatcagttc aatcacgcct ttacgcggta
    34381 cgatcaccga atggcttggc aaagattgac caattggcat tgaacagacc gccagacggt
    34441 ggccgtcggt tgccacggtg cgcagttctt caccttcggt ttcaaacagc ataccattta
    34501 agtaatagcg aacgtcctga tgcgccatcg aaaactgggt cgcttcaatc agacgcttca
    34561 tcgttgcctg cggcagggta aattcgactt cactctgcca gtcatcgagg ttcgggaaat
    34621 ccgccgctgg cagggtagac agcgaaaaac ggctacgccc ggagcgtacc agcatccgtt
    34681 caccttccag ctgcacggca atttccgcgc cttcaggcag accacggcag atatcaaaga
    34741 atttgcgcgc cggaacggtc gtcgctcctg gctcgtgtgg ctgaaccagc gcaacacgtg
    34801 ccaccatttc catctcgaga tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta
    34861 acagcagatt accgagaatc ggtagcgtag gacgaccacc taacggaccg ctcacctgtt
    34921 gtagcggttt taataaatgc tcacgttcta cggtaaattt cataggttta cgatgacaat
    34981 gttctgatta aatttgaaaa atcttctttg atatcgtggc tctcttcacg caactgctcg
    35041 atcttacggc aggcatgaag caccgtcgtg tggtcacgac caccaaacgc atcgccaatc
    35101 tccggcagac tgtggttagt cagctctttc gccagcgcca tcgccatctg gcgcggacgc
    35161 gccaccgagc gggatcgacg cttggaaagg agatccgcga ctttgatctt gtagtactcc
    35221 gccaccgtct tctgaatatt gtcgatggtg accagttttt cctgcaatgc cagcaagtcg
    35281 cgcagcgcct cacgcacgaa gtcgatggtg atcgcccgtc cggtaaagtt ggcattggca
    35341 atgacgcggt tcagcgcccc ttccagctca cgtacgttag atcgtagacg cttggcgata
    35401 aagaacgcca cttcgcccgg caaacgaatg tcgttttcgt cggccttttt catcaggatc
    35461 gccacacggg tttccagctc tggcggttcg atcgccacag tcagtcccca accgaagcgg
    35521 gatttcaaac gatcctcaac gccgttgatc tctttcggat agcgatccga ggtgagaatg
    35581 atctgttgat taccttccag cagggcgttg aaggtatgga aaaactcttc ctgagatcgt
    35641 tctttattag caaaaaactg aatatcgtcg atcagcagtg catctacgga acggtagtag
    35701 cgtttaaact cttcgatcgc gttgttttgc agggctttaa ccatgtcctg aacaaagcgc
    35761 tcggagtgca tataaaccac tttggcattc ggcttgcgcg ccataatgcc gttacccacc
    35821 gcatgcagca ggtgagtttt acccagaccc gtgccgccat aaaggaacaa cgggttatag
    35881 gcaccgccag ggttatccgc cacctggcga gccgccgcgc gcgccagttg gttagattta
    35941 ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt tagaacgata ggtcggttct
    36001 gccggggccg ggacgttatc ccaacctgag cgcgtagaag gcgcagcacg ttgcggctgc
    36061 gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc
    36121 gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg catccgctcc gcagaaactg
    36181 gttagcagtc cattgatatt attaaggtac ttgtcccgta cccaatcgag gacaaaacgg
    36241 tttggcgcgt acagggccag cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac
    36301 atactgaatt ctgtggctgg taactcatcc tgcaatcggg caagacactg ctgccaaagc
    36361 gaaagtgaca cggcggactc cactcgaaca aaagtcgata atgactaagg ctgaaacatt
    36421 catgattgtt gacgtacgtc gaaaagaccc tgcttgaggg tgacgcacga accgctgtct
    36481 gcggttatat gcccgatcaa gatcctgcaa aacgatcggg accgcggatc atagcctaaa
    36541 ctgcgcaaga gatcttctgt ttctcacaga ttttcccgat ttatccacag gactttccag
    36601 aactcgctaa gtgtaaacga tcctgccgcg aggcgggcac gatttacgcc gcatattgga
    36661 aaatttaatg accatagaca aaaattggct taatcgatct aataaagatc caggacgatc
    36721 cttgcgcttt acccatcagc ccgtataatc ctccacccgg cgcgccatgc tggtttccac
    36781 tggtgtgagg tcgtacattt tccctgcgaa aaggtgcgga aaagcgcggt aaataaggaa
    36841 agagaattga ctccggagtg tacaattatt acaatccggc ctctttaatc acccatggct
    36901 tcggtgtcca tcgtttcatt tttcggcgga tatccaataa agccattgaa tttattcaag
    36961 tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt
    37021 ctcacggctt ccgtgctcgt atggctacta aaaatggtcg tcaggttctg gcacgtcgtc
    37081 gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt
    37141 aagctcgcat ttcccaggga gttacgcttg ttaactccca gtcaattcac attcgtcttc
    37201 cagcagccac aacgggctgg cacgccgcaa attaccattc tcggccgcct gaattcgctg
    37261 gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc
    37321 aatcggatta aacgtctgac gcgtgaaagc ttccgtctgc gccaacatga actcccggct
    37381 atggatttcg tggtggtggc gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg
    37441 gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg ctcgcgggtc ctgatagccc
    37501 tcattcgggt ctatcaacgc ctgattagtc cgctactcgg gccgcattgt cgtttcactc
    37561 caacctgttc aagctacgga attgaggcat tgcgcaggtt tggagtgata aaaggcagtt
    37621 ggttgacggt gaaacgcgta ttaaaatgcc accctttaca ccctggtggt gacgatcccg
    37681 tcccgcccgg accatttgat accagagaac actaacgatg gattcgcaac gcaatctttt
    37741 agtcatcgct ttgctgttcg tgtctttcat gatctggcaa gcctgggagc aggataaaaa
    37801 cccgcaacct caggcccaac agaccacgca gacaacgacc accgcagcgg gtagcgccgc
    37861 cgaccagggc gtaccggcca gtggccaggg gaaactgatc tcggttaaga ccgacgtgct
    37921 tgatctgacc atcaacaccc gtggtggtga tgttgagcaa gctctgctgc ctgcttaccc
    37981 gaaagagctg aactctaccc agccgttcca gctgctggaa acttcaccgc agtttattta
    38041 tcaggcacag agcggtctga ccggtcgtga tggcccggat aacccggcta acggcccgcg
    38101 tccgctgtat aacgttgaaa aagacgctta tgtgctggct gaaggtcaaa acgaactgca
    38161 ggtgccgatg acgtataccg acgcggcagg caacacgttt accaaaacgt ttgtcctgaa
    38221 acgtggtgat tacgctgtca acgtcaacta caacgtgcag aacgctggcg agaaaccgct
    38281 ggaaatctcc acctttggtc agttgaagca atccatcact ctgccaccgc atctcgatac
    38341 cggaagcagc aacttcgcac tgcacacctt ccgcggcgcg gcgtactcca cgcctgacga
    38401 gaagtacgag aaatacaagt tcgataccat tgccgataac gaaaacctga acatctcttc
    38461 gaaaggtggt tgggtggcga tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa
    38521 cgacggtacc aacaacttct ataccgctaa tctgggtaac ggcatcgccg ctatcggcta
    38581 taaatctcag ccggtactgg ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg
    38641 ggttggcccg gaaatccagg acaaaatggc agctgttgct ccgcacctgg atctgaccgt
    38701 tgattacggt tggttgtggt tcatctctca gccgctgttc aaactgctga aatggatcca
    38761 tagctttgtg ggtaactggg gcttctccat tatcatcatc acctttatcg ttcgtggcat
    38821 catgtacccg ctgaccaaag cgcagtacac ctccatggcg aagatgcgta tgctgcagcc
    38881 gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa cagcgtatca gccaggaaat
    38941 gatggcgctg tacaaagctg agaaggttaa cccgctgggc ggctgcttcc cgctgctgat
    39001 ccagatgcca atcttcctgg cgttgtacta catgctgatg ggttccgttg aactgcgtca
    39061 ggcaccgttt gcactgtgga tccacgacct gtcggcacag gacccgtact acatcttgcc
    39121 gatcctgatg ggcgtaacga tgttcttcat tcagaagatg tcgccgacca cagtgaccga
    39181 cccgatgcag cagaagatca tgacctttat gccggtcatc ttcaccgtgt tcttcctgtg
    39241 gttcccgtca ggtctggtgc tgtactatat cgtcagcaac ctggtaacca ttattcagca
    39301 gcagctgatt taccgtggtc tggaaaaacg tggcctgcat agccgcgaga agaaaaaatc
    39361 ctgattcggt gagttttcgc taaaataagg gcggtcagtt gaccgccttt tttcttttcg
    39421 tagggcggat aagcaccgcg catccgccac acaaagcaac aggaacatca tgagcgataa
    39481 tgacactatc gtagcccagg ccacgcctcc gggacgtggc ggcgttggca tcctgcgcat
    39541 ctccggcttc aaagcccgtg aagttgccga aaccgtgctg ggtaaactac ctaagccgcg
    39601 ctacgccgat tatcttccgt ttaaagacgc cgacggcagc gtgctcgatc aggggattgc
    39661 gctatggttc cctggcccga actcgttcac cggcgaagat gtgctggaac tgcaaggtca
    39721 tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg
    39781 gattgctcgc cctggtgagt tttccgaacg cgcgtttctt aacgataaac tcgacttagc
    39841 ccaggccgag gcgattgccg atcttatcga cgccagttcg gaacaggcgg cccgttcggc
    39901 acttaactcg ctgcaaggcg cattctccgc acgggtcaac catctggtgg aagccctcac
    39961 ccacttgcgc atttacgtcg aagcggcaat tgatttccct gatgaagaga tcgatttcct
    40021 ctccgacggc aaaattgaag cccagcttaa tgacgtgatt gccgaactcg atgcagtgcg
    40081 tgctgaagca cgtcagggta gtttgttgcg cgaagggatg aaggtggtga ttgccggacg
    40141 tcctaacgcc ggtaaatcga gcctgttaaa cgcgctggcg ggccgtgaag cagcaatcgt
    40201 aactgatatc gccggaacca cacgtgacgt gctgcgtgag catatccaca ttgacggaat
    40261 gccgctgcat atcatcgata ccgctgggct acgtgaagcc agtgacgaag tggaacgtat
    40321 tggtatcgag cgcgcgtggc aggaaattga acaggccgac cgcgtgctgt ttatggtcga
    40381 tggcaccaca acagacgccg tggatccggc agagatctgg ccggaattta ttgcccgtct
    40441 gccagcgaaa ctgccgagca ccgtggtgcg caataaagcc gatatcaccg gcgaaacgct
    40501 gggaatgagt gaagtgaacg gtcacgcgtt aattcgtctc tcggcaagga ctggtgaagg
    40561 cgtggacgtg ctgcgtaacc atctcaaaca gagcatgggc tttgacacca acatggaagg
    40621 cggcttcctg gcgcgtcgtc gccacctaca ggcgctggaa caggcagcgg aacatctaca
    40681 acagggcaaa gcgcaactgt tgggagcctg ggcaggtgaa ctgctggcgg aagagttgcg
    40741 tctggcacag cagaacttaa gcgaaatcac cggggaattt acttcagacg acctgctggg
    40801 gcggattttc tccagcttct gtattggtaa gtaaccgcgc ttacgaagcc gtattctgac
    40861 tgtcagatgc ggcttcgctt cattgttagc actcctgtta ttcctcaacc ctttttttaa
    40921 acattaaaat tcttacgtaa tttataatct taaaaaaaag catttaatat tgctccccga
    40981 acgattgtga ttcgattcac atttaaacaa ttttagaata gacaaaattc ttgagtatta
    41041 tactgtagcc tcgtgtcttt cgaggataag tgcattatga atatcttaca tatatgtgtg
    41101 acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta atttaccctt
    41161 ctgtagccat caccagagcc aaaccgatta gattcaatgt gaacgattag tttgctatat
    41221 cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta gtaaatgatg
    41281 gtgcttgtat atatacatgg cgaattaatg gatattgcag atgtaatatt cacagggatc
    41341 actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc tccctgaacc
    41401 gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc gtgaagaagc
    41461 aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt ttatcgattt
    41521 actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga tgatgcgcgg
    41581 cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag tgaaaaatat
    41641 ctttggttat caatacacca ttccgactca ccagggccgt ggcgcagagc aaatttatat
    41701 tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca aaatggtggc
    41761 attctctaac tatttctttg ataccacgca gggccatagc cagattaacg gctgtaccgt
    41821 gcgtaacgtc tatatcaaag aagccttcga tactggcgtg cgttacgact ttaaaggtaa
    41881 ctttgacctt gagggattag aacgcggtat tgaagaagtt ggcccgaata acgtgccgta
    41941 tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac tggcaaactt
    42001 aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg actccgcacg
    42061 ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag actggaccat
    42121 cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt ccgccaagaa
    42181 agatgcgatg gtaccgatgg gcggcttgct gtgcatgaaa gacgacagct tctttgatgt
    42241 gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggg ttcccgacat atggcggcct
    42301 ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga atctcgactg
    42361 gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag agattggcgt
    42421 tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac tgctgccgca
    42481 tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata aagtcgccgg
    42541 tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa ccggtaaaca
    42601 actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat atactcaaac
    42661 acatatggac ttcattattg aagcctttaa acatgtgaaa gagaacgcgg cgaatattaa
    42721 aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa aactgaaaga
    42781 agtttaatta atactacaga gtggctataa ggatgttagc cactctctta ccctacatcc
    42841 tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag cagaaaaaaa
    42901 gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg gaggtatgtt
    42961 tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta tccttatcat
    43021 tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt taaattatcc
    43081 tgtcggctcc agttttaaca ccatcaccaa agatttaatc ggtaacacct ggaacattat
    43141 cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata tctctgctaa
    43201 tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta atccacgtat
    43261 tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc
    43321 cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga tcgtgtttgg
    43381 ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca ccactgcggg
    43441 aacgtcttac ttcccgtata tctttatggc tttgccgctg tgtctggcgt catttggttt
    43501 ccacggcaat attcccagcc tgattatttg ctatggaaaa cgcaaagata agttaatcaa
    43561 aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac
    43621 gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg gcaacgttga
    43681 ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt tttgcctgct
    43741 ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg ggttgttcga
    43801 ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca aaaccgtgct
    43861 gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct ttatttacgg
    43921 gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg cagtgcttgc
    43981 aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat
    44041 tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg gcaacgtctt
    44101 taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc tggcttcttg
    44161 cctctcagga aatcacttat gtccaaatgg caactcgcct gattctcctt caccacgtat
    44221 gctttgcgtc accttactat taggacgctt tagcccatgt cccgcttttt aatttgtagt
    44281 tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg tttaccgcgc
    44341 atcgccgacg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc cgtttatctg
    44401 gcgggcatgg cagctgcgat gttatttgcc ggtaaagtgg ccgatcgttc agggagaaag
    44461 cctgtcgcca tccccggcgc ggcgctattt attatcgctt cggtgttctg ttcactggct
    44521 gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc aggctgttgt
    44581 tacgtagtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg ggctaaagtg
    44641 ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt gctcggacat
    44701 ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat gatgggcatc
    44761 gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc ccccgctgct
    44821 tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc ggtttttcct cagccgtgtt
    44881 gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc gccggtattg
    44941 ctgatggaaa tcatgggttt tgagcgcgga gaatacgcca ccattatggc gttgactgct
    45001 ggcgtcagca tgaccgtttc attctccacg ccatttgcgc tgggaatttt taagccacgt
    45061 acgttgatga tcacctcgca ggtgttattc cttgcagcgg ggatcaccct tgccgtttca
    45121 ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt ctcggtaggt
    45181 tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg cgtagccagc
    45241 tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct ggcagcggtg
    45301 gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag catagtgagc
    45361 ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga agaaatccat
    45421 caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg agcgcagcgt
    45481 gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt cgctggcaaa
    45541 gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctgggcc tgtcgcccac
    45601 accgctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga gcaatctgct
    45661 gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg cggcggaatc
    45721 gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac gttacccgca
    45781 ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta ctcgtggtga
    45841 agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc tgttaagctc
    45901 gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg tctggttacg
    45961 caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac gttatccgca
    46021 tatcagcatt tgctgggaac agagcgacac ctgggcgctg gacaatgtgt tacaggagct
    46081 ggggcgcgaa cgcacgattg ctatgagcct gccggaattt gagcagtcac tgtttatggc
    46141 agcgcaaccc gacaatctgc tactggcgac cgcgccgcgc tactgtcagt actacaatca
    46201 actccatcaa ctgccgttgg ttgctcttcc tctcccgttt gacgaaagcc agcaaaaaaa
    46261 gctggaagtt ccttttaccc tgctgtggca taaacggaac agccataatc cgaagatcgt
    46321 ctggttacgg gaaaccatta aaaaccttta cgcgtcgatg gcataaccga atcgtatgaa
    46381 acgcgaccca atttcacaat aaaatgtaaa aaagttgtaa taagcttgtc tgaatcgaac
    46441 ttttagccgc tttagtctgt ccatcattcc agtaaatgat tactcttgta ttcataatgg
    46501 accattaagc atggagcgaa aaatggcgac tcactttgcc cgagggattt taacggaagg
    46561 acatctgatt tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa acattccccc
    46621 tcatcgtcaa agccgttttc tggcgtctag aggtttactc gcagaactga tgttcatgct
    46681 gtatggcatt ggcgaattgc cggaaatcgt caccctgccg aaaggtaaac cggttttcag
    46741 tgataaaaat ttgccttcgt tttctatttc ctatgccggg aatatggttg gcgtggcgtt
    46801 aacaaccgaa ggtgaatgtg gtctcgatat ggaactacag cgtgcgacgc gcgggtttca
    46861 cagcccacac gcgcccgata accacacctt ttccagcaat gaatcgctat ggatcagtaa
    46921 acaaaacgat cctaacgaag cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa
    46981 actaaccggt gatgttttga atgacgatcc gcgcgatctg cagctgctgt ccattgccgg
    47041 acgcctgaaa tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct
    47101 ggtgtggtcc gtggcggtca cgcccacgat tgaaaagctc agtgtctggg agttcgatgg
    47161 caaacacggc tggaaaagcc tgccggatat tcacagccgc gccaacaatc ctaccagccg
    47221 gatgatgcgt tttgcccagc tctctaccgt gaaggctttt tcgccaaatt gatagacaac
    47281 cacaggagtc atcatgtctg aaaaattgca ggtggttacg ttactgggga gcctgcgcaa
    47341 aggctcattt aatggcatgg ttgcacgtac cctgccgaaa attgctccgg cgagcatgga
    47401 agtcaatgcg ttaccttcca ttgccgacat tcccttgtat gacgctgacg tacagcagga
    47461 agaaggtttt ccagcaacgg ttgaagctct ggcggaacag atccgtcagg ctgacggtgt
    47521 ggtgatcgtc acgccggaat ataactactc ggtaccgggt gggctgaaaa atgccatcga
    47581 ctggctttcc cgcctgccgg atcaaccgct ggccggtaaa ccggtgttga ttcagaccag
    47641 ctcaatgggc gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt
    47701 cctcgatgca atggtgatga acaagccgga atttatgggc ggcgtgattc agaacaaagt
    47761 tgatccgcaa accggagaag tgattgatca gggtacgctg gaccacctga ccgggcaatt
    47821 gaccgcattt ggtgagttta ttcagcgagt taagatctaa ataaaaaacc cgccagcaat
    47881 catgcatggc gggtttttaa cgcgctatcg attttagtga gcgtcgataa agacaatctt
    47941 caggataaac agcagcgcaa cgatgattac gcacgggctt agatcacgca gacgtccggt
    48001 accgattttc atcacgcagt aggagataaa gcccagcgcg ataccttcgg taatcgagaa
    48061 gctgaacggc atcatcacgg cggtaataaa cgccggaaca gattcagtaa gatcctgcca
    48121 gttcacgcgt gccagactgg aagtcatcag cacgccaacg taaatcagcg caccagctgc
    48181 agcgtagcct ggcaccatcc ccgccagcgg cgacagaaag ataaccagca ggaacagcag
    48241 accaacaacc actgccgtca gaccggtacg accgccaacc gatacgccgg aagaggactc
    48301 aatataagcc gtaacggaag aagtaccgat aaacgaaccg gtcacggaag agatactgtc
    48361 gacatacagc gcctgcttca tgcgcgggaa tttacccttc tcatccgcca ggcctgcttt
    48421 atcggtcacg ccaatcagcg taccggagga gtcaaacagg ttgaccagca tgaaagagaa
    48481 aatcacccct gccagcccga ggttaaacga cccggctaaa tctacatgac ccacaactgt
    48541 cattacgctc ggcggcgcag aaacgatgcc attgtagtgc acatcaccca gcatccagcc
    48601 cagcagcgtc gtcaccacga tagaaaccag caccgctgcg tgaatgttgc gcgaggccag
    48661 aatagcaatg atgaagaagc cgaggatccc cagcagcacg ctgtgagaag tcaggttacc
    48721 gatgctgacc agcgtttccg ggttagcgac aatcacacct gcattcttca gtcccatcat
    48781 accaatgaac agaccgatac cgctggtgat gcccacacgc agacttaccg gaatgttggc
    48841 aatcatccag tagcgaacgc ggaaaatcgt cagtaacagc agaccaatcg caccccagaa
    48901 gattgcgccc atcccgactt gccacggcaa gcccatcgcc tgtacaacga caaaagcgaa
    48961 gaacgcattc aggcccatag cgggtgccag tgcaactggc aggttagcaa acagtcccat
    49021 cataatactg ccgaatgcag cgatcagaca ggtagtgacg aagacggcgc tggtatccat
    49081 gccagcaacg ccaagaattt gcgggttaac aaaaacgatg taaaccatcg tcaggaaggt
    49141 ggtaaaaccg gcgatcactt cggtccgtgc cgtcgtgcca tgttcgcgca gtttaaacac
    49201 gcgttccagc atcccctggc cagaagcctg ggttgtgtgt tgttgactca ttatctattt
    49261 ccgaacaagg agggaaaatc cgtcgctatc gtataccaaa atgagacaat atggacggct
    49321 gaagataact ttttttcatt gtatttgctt atacggcaac gattgcgtca tgcgaattgg
    49381 catgacgaaa acgttaaact aacgataaag gaaagacatg tcccagatag aagcggtatt
    49441 tttcgactgc gacggtacgc tggtcgacag tgaagtcatt tgctcccgcg catatgtaac
    49501 gatgtttcag gaatttggta ttacgctcga tcctgaagag gtattcaaac gtttcaaagg
    49561 tgtaaaactg tacgaaatta tcgatattgt ttcccttgaa catggtgtta cgttagcgaa
    49621 aacagaagct gaacacgttt accgtgcaga agtcgctcgg ctgttcgatt cagaactgga
    49681 agccatcgaa ggggctggag cgctcctgtc agcgatcact gcgccaatgt gtgtggtatc
    49741 taacggccca aataacaaaa tgcagcattc tatgggcaag ctgaatatgt tgcactactt
    49801 cccggataaa ctgttcagcg gctacgatat tcagcgctgg aagccagacc cggcgttaat
    49861 gttccatgcg gcaaaagcga tgaatgtaaa tgtagaaaac tgcattctgg ttgatgactc
    49921 agttgccggt gcgcaatctg gtatcgacgc aggtatggaa gtgttctact tctgcgccga
    49981 cccgcacaat aagccgatcg ttcacccgaa agtcaccacc tttacccatc tttcgcagtt
    50041 accggaactg tggaaagcgc gtggttggga tattacggca tagttcttca cactcccttc
    50101 acttaccccg cttaaattgg cgctcaaagg taagtaaagg gagtttgata tgtctgtttc
    50161 acgtcgggta atacatcacg gactttattt tgcggtttta ggaccgttaa ttggtgttct
    50221 gtttcttgtc ctctacatat tcttcgcaaa agaaccgctg gttcttttgc gaagaataca
    50281 ggtacttcca ttgtttctct tattgtcgat tactacggga gttattcctg cattgttaac
    50341 cggcgtgatg gtcgcctgtc tgccggaaaa gatcgggtca cagaaaaatt atcgttgtct
    50401 ggctggtggc ataggtggcg tcgttatcac cgagatctat tgtgcagtta ttgtacatat
    50461 taagggcatg gcttcctcgg agttgtttga aaacattctt tctggtgaca gtctcgttgt
    50521 ccgcatcatt cctgcattgc tggcaggtgt ggtgatgagc agaatcatta cccgtctgcc
    50581 cggattggat atttcatgtc ctgaaacaga ctctttaagt taagcgggat actttatctt
    50641 tgggctactc aaaagcagac aggatgttac tatgactcaa aatatcaggc cgttacccca
    50701 attcaaatat catcccaagc cactggaaac aggcgcattt gaacaggata aaaccgtaga
    50761 gtgcgattgc tgtgaacaac agacgtcagt ttattactcg ggtccctttt attgcgttga
    50821 tgaagttgaa catctctgtc cgtggtgtat tgcggacggt tctgctgctg aaaagtttgc
    50881 aggtagtttt caggatgatg ccagcataga aggtgttgaa tttgagtatg atgaagagga
    50941 cgaatttgcc ggtattaaga acacatatcc tgatgaaatg ctgaaagagt tggttgaacg
    51001 cacgccaggt tatcatggat ggcaacagga attctggctt gcgcattgtg gcgatttctg
    51061 tgcttttatc ggctatgtgg gctggaatga tataaaagat cgcctcgatg aatttgccaa
    51121 ccttgaagaa gattgtgaga atttcggtat tagaaattct gatctagcta aatgcctgca
    51181 aaagggtggt gattgtcagg gttatctctt ccgctgtctc cactgcggca agctgagact
    51241 gtggggtgat ttttcgtagt tatttaaata atgagaacag gccggagcgt aattcataca
    51301 tccggcctta tttcttcagc ttaaccgagc gctaattctg cggcagcggc tttatcagcg
    51361 attaccatca gcgaaggatg cagctgcaac acggaagctg gaacatcttc ggtaaccgga
    51421 ccttgcagaa catttttcag tgcctgcgct ttacctgcgc cactgacaat tatcagcaag
    51481 tttttcgccg ccatgatgct tttcggcccc atcgtcacgt agctgtcagg cactaacgaa
    51541 aagtcgccgc ccaactcacc atgcgccaca atatcaacca tctctccctg gatcgggaac
    51601 tccacggttt gctcatggaa atgggtcgta ttcggcaaat tgccacaaaa atgaccatct
    51661 gcacctaatc ccagcaccac caaatccagg ccgccttcac gcgccagttt ctgatcatgc
    51721 tcgcggtagt tatcaatagt gagcttctgg atattctctt ctttgatccc cgcaggggtg
    51781 aaaaacagat tacgcagatt ggtaatcgtt acaccttctc cctctttgcc gcgaaaggga
    51841 atttcatcga aattatagaa atagcagtta tcgtaccagg gcttaccttt aaccagggtg
    51901 gtgaggtatt catacatgcc tttgggcgtg ctaccggcgg taattgccag gttaacacga
    51961 cgcgtcttcg acatataacc aagcagatgg tgtgccgcga cacggctcat ttcctggtaa
    52021 tcttcggtaa tgattaattt catcttagtg ccttatttta tttaaataat ttctgtacga
    52081 aggctgcata ggcaggccgc cagacatcca tttcatggtt caggccggga tattcctggt
    52141 aatcaaactt aatttttttc tgctcaagct cagttttcag cccggcgata tccttgccgg
    52201 ttacgacatc tttatcacca acgaccacag taaaattacg tagttgctgg ttaatagctg
    52261 ccggatcgtt cagccgggcc gcgacacctt catccggtac ggttgtcgtg gtaacaccac
    52321 tgaatgtggc cagccagcca aagctttcca gatgattcat tcccgatacc agcgcctgat
    52381 acccaccttg tgaaagccct gccagtgcgc gaccatcggc atctttactg acgttaaaac
    52441 gcttgctaat cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattca
    52501 gcggataaaa gactttacgt ctttcctgag gcacgaaatc ttcgggaata atgcccttcg
    52561 catcggtttc ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca
    52621 ggttatccat gatttgcggg atacgcccct gatcgatagc ggaacgtccg gtatcaccaa
    52681 aaccgtgata gaaatagagc actggcaaag gctcgcccat gccggtgtat cctggcgggg
    52741 tccagacata catctgacgt tcagattgca atgcgttgga gtggtaagtt atggcgatca
    52801 aatcaccatg cgcaacagaa cgcgtatcca gataactgcc tggcaccaga atcatactgg
    52861 agttaacctg gcgctgaggc ttagtcattg cggtgcctgt atcaatgctg cgtacaccat
    52921 caacattgaa aaaatactcg tacaggttgc ctttcaggat aggtgtgcgc cacgaccaga
    52981 ctcctgcttc gtctttggtc atcgggtgaa tattgtccag aaccggaaca cccaccacta
    53041 cagagacatt ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggcattga
    53101 cctgagtcac atactgtttc accgggatag tgggtgccgg gctggctggc atatccgctg
    53161 ctatggccca ctgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta
    53221 tattcatcgt aatattcctt tcattaagtt ccattaccac cagatttcag cctgggcacc
    53281 gacagcaaac tggtcgtctt tattgtcttc gaaggtgaag ccatccagtt cgttttccag
    53341 ggctttgata tacgtggcgt agaaacgaat ttccggacgc gaggtcaaca tactggtatt
    53401 gactttaaag gtatggaaga gcgtggtttt ataaccagac tcattaaatt tattactatt
    53461 cgcatctttg ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc
    53521 ccaaatatag gccgggcgaa caaccgcacg aatagattcg aaatcagagt gtgcgcctgt
    53581 ttcgtagcta taaatatcgt taccgaagga gtaaacaatc gcgttagcca caatgaaatg
    53641 atcgccgata taggcttcgc cctgtgaagt cagacgaact gctgttccgc cggtgtgatc
    53701 accataataa cgaccattaa aggtggtaaa tggacttgcg ccagcataac ggccaaagtt
    53761 actggcgata gagttattcg caaccaggaa ggagaattcg ttgaagccac ctttatcaaa
    53821 tttctgcgtt aaagatgtgc caaacatcca ggtatctttc cagtcataat agccgttatt
    53881 atcctgatta tctttttccg atgcgctttc gtttgccgtg acataacgac cactcaccat
    53941 taaggtcgct ttatcccata acgggatatc tttatagcgt aaatcaatag cattggtatt
    54001 aatctgctgt ttgttttgca ggctgcgatc gtaatcatcg acatcttcgc gaaccagcgc
    54061 gatatcaatt ttacccggac cgactttcca gttttccaga cctacacccg ctgcggcatc
    54121 agtacgctgc gttttccagt caagcatctg gatttcaatt ttcggcgcac cgtgtttacc
    54181 cacccagaaa tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc
    54241 ggagaactga ataaagttct cgccaccggc gttatcgcca aaccagccgg tagagtactg
    54301 ctgaccaacg ttaccatcca tcatcacaac ggcatcaacc cgtttgccgt tttcgttgta
    54361 gacacgttgt tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag
    54421 agaaccaatc gcccatgatt taggtgaacc atgagaggcg gtcccccaac cagaacggta
    54481 atagccgttg taactaaagc caatttcatc tttcacaaac ttactgaaat ctttcagagt
    54541 catggaagaa taaatcgagc ctgtctcact ggcatttttc tcttcatttt tgaccagtac
    54601 agcatcaggt tttgcggcac tactggtcgg gaacggattg gcggcatacc cttgatcatt
    54661 cgtacttacg ctacgattca ccgtcgctgg cgtatatttt ttcttctctt catctttata
    54721 ctttttgagt tcgctttgcg tttctcttaa cgctttttct aataactcaa ggcgatgttc
    54781 caccgttaat gattcagcca atgattgtgc agaaaaggct aacggtgcca ttaataagat
    54841 ggcagaggta ataagatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt
    54901 aacgtatcgc atccagactg ttctgaatgc gacgataatt aaggtgcttt attggttatt
    54961 tttttaatga cagcccccgc gttttgatca cttctgcata ccatccgaag cttttcttac
    55021 gcgttcttgt gaggcttcct tcgccattat catcacgatc cacataaata aagccgtagc
    55081 gcttagacat ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc
    55141 tcataatatc cacaccatcg gcaatcgctt catttacctg taccaggtga tcgtttaaat
    55201 aggcaattcg ataatcgtcc tgtatcgaac catccgcttc aacgctgtct tttgcgccta
    55261 atccgttctc gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa
    55321 cccgtaatcc aaccggatca atttgccacc cccactctga acttttcaga tgcggattgg
    55381 ggatcatatt cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac
    55441 aaccagtcat gtaataacta aaagagatga aatctacggt atgttttaaa tcttctgcgt
    55501 cactttcagt catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg
    55561 gatactgacc acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg
    55621 cctccaacat atcctgtggc tggcaggtga gggggtaaac cagcccgccg agaagcatat
    55681 tgccgatttt cgcttcgggg atcaggctat gacaggcttt aactgcccgc gcactggcaa
    55741 ccagttgatg atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca
    55801 cgcccgtgaa tggcgcgtgt aacgacatat tgatttcatt aaacgtcagc cataacgcta
    55861 ctttatgttg gtagcgggta aagaccgtgc gggcgtaatg ctcgaagtga tcgatgaccg
    55921 cccgattagc ccaaccgccg tagtttttca ccagcccata tggcatttcg taatgggata
    55981 acgttaccag cggcttgatc cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg
    56041 ctaaccccgc ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa
    56101 tggaaatacg cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac
    56161 ggtgataaaa atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca
    56221 tttttcccat tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcca
    56281 gccaggcacc ttccacctga ttggcagctg ttgcgccacc ccaaagaaat gtttctggaa
    56341 atgctttcat aattaactcc ttttatcgtt agcgaatgat ggataacagc ggctcaccag
    56401 cgcttatctg cgccgtgccg tggggtaata cgtctgtaaa atcatcgcta ttactgatta
    56461 ataccggcgt cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca
    56521 gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt
    56581 ccagttttac ggtgtcgata ccgacatgaa tcaggatctc cacaccatca tctgactcaa
    56641 tgccaatagc gtgtaatgtg gcgaacaacg aagcaattcg acccgcaacc ggagaacgca
    56701 cttcaccaac cgagggcaga atggcgatac ctttacccaa caggccactg gcaaacgtgg
    56761 tatcagcgac gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag ataccgccct
    56821 gctcaggtgg tgtaataacc tctggtgttt tctcttgcgg ggcaccctgc gctggctgac
    56881 gtttagcggt gatgaaatga agcatcaccg taccgacaaa tgcgcaaccg atggcaatga
    56941 caccgccaat aacgctggcc cagacggtga aatcaatccc cgttgacggg atggtttgca
    57001 tgaaggtgaa aatacttggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga
    57061 tggtggcccc caatgctcca ctgatacagg cgataacgaa gggatactta cgcggcaggt
    57121 tgacgccata taccgctggt tcggtgatac caaacaggcc cgtcaacgcc gctgatcccg
    57181 ccaccacttt tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc gccccgactt
    57241 gcgccataat ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt
    57301 tattgataca cagcggcacc aggccccagt gcagtccgaa catgacgaag atttgccaga
    57361 aaccgcccat taccgcgccc gcaaatgcag gaaccgcctg ataaagccag agataaccgg
    57421 cggcaatcat ttcgcttatc caggttgata gcggccccac cagcaaaaag gtgacgggtg
    57481 tgataaccat cagacatagc aatggtgtga agaaattttt gattgccgac ggtaaccacg
    57541 cattaagtcg gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg
    57601 atgacgagta attcaacaat gtgaccggaa tacccaggaa atccagcccc agctcatccg
    57661 ctttttgccc gttctcgaaa gcagtcagaa ttaatggatg cactaacgct ccaccaatca
    57721 ccatggcagt aaatggatta ccgccgaagc gtttccccgc ggtgtatccc aggattatcg
    57781 ggaagaacca aaacaaggca tcactggcgc tgaataaaat taaataagta ccactttgtt
    57841 cggtcgtcca ctgaaaagtg agcgccagag ccagcatacc tttcaggatc ccggttgccg
    57901 ccatcaaacc gatcagaggc gtaaaaatac ctgaaataac ataaacaaag cggtttagca
    57961 gattaccttt atcatcattt tccggtgcct gttgcgcttt ttcgtcaagg cctgccacac
    58021 tgttaaccgc caggaagaca tcggccacat ggttacctat gaccacctga aactggccac
    58081 cgctttccac caccataata ataccggggg tctttttcag tacctctgct tgcgctttgc
    58141 tttcatcctt taacttaaaa cgtaatcgcg ttgcgcaatg catcagacta acaatgttat
    58201 ctgcgccccc gactcctgcg actatttttc tggctaactc cgtcataact tgccctctac
    58261 cgctttgcgg caaaactcaa aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca
    58321 attttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta ataatatttc
    58381 agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct gcgggtgaga
    58441 ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ctccgcacat tgccatgctt
    58501 gcgggtaatt ttgttttact gcttgttgta atgactcatc actatcgtta attgaagcat
    58561 gtccaagaat acgccaggat aaaaacttca gatgcgtaac cagtcgctga taactcaagc
    58621 tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt tcgcgcatta
    58681 attgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg accagatgca
    58741 tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta tcaataatgg
    58801 ttaacgcttc ttcccctagc tggaactctt tcgggtaaag ccgctggata tcccacagca
    58861 gcgggttggg cagtaacacg ttttgctgaa agcgtttaat cgcaaactgg caatggtcag
    58921 ttagcgagat ataaatactg tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa
    58981 tacgatcaca ggttgccatc acctcaagag gaatatgact taagagttcg cttaatcgcc
    59041 cgttcagttc atgactgctc aaggcatact ctttttctat tccacttgag ttaattcttt
    59101 cgccagcgcg tttttgaaag ccaattccgc gccccatgac gactttttcc cgctgttgat
    59161 catcaataac caccacaaca ttattgttga gaattttggt gatttgcatg ttcatagcaa
    59221 ggaccttttt tataaacaaa aaaacccgac ttcaccagta ttctctggtt atgtcaggtt
    59281 ttgcctgcga atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca
    59341 ggttcggaaa ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata
    59401 tgaccatgct cgcagttatt aactttgtgt aattttagga atttataaag ttatatataa
    59461 caaatcccaa taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatctttcc
    59521 ccgccagcag tttatccagc tcatcgccac cgacgtgacg gaaatcctgc cccttcacgt
    59581 agtagaagat aaactcacaa atattctggc agcggtcgcc aatacgttcg atagaacgcg
    59641 cgcagaacag cgcagtaagt acgctcggaa tggtacgcga atcttccatc atgtaggtca
    59701 tcagttgacg aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac
    59761 gtaccgcttc gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga
    59821 tggtatgacg gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt
    59881 tctccagcgc agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa
    59941 tggttttact gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga
    60001 taatgcgcac gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt
    60061 cgatcacgcg cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa
    60121 gctgctgctc caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg
    60181 cgttgaactg gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa
    60241 tcaaccgtaa cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag
    60301 atcgtccgtg ttgctgaact caatcaattc gcccaggtac ataaacgccg tgtggtcgga
    60361 acaacgcgca gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag
    60421 ttcggtgatc agctcttcaa tacgcccggt agagataggg tcgagcgccg aacacggttc
    60481 gtcgagcagc agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg
    60541 ctgaccacca gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa
    60601 tgcggctttg gtcaatgccc actgcacgcg ctcgtccatg tcggcacggg agagcttctc
    60661 aaacagacga acgccaaaag cgatgttgtc gtagatggac atcggaaacg gcgtcggttt
    60721 ctggaacacc atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat
    60781 gttgtcgcca tcaagcagaa tttcaccttc tgcgcgctgc tccggataca gttcaaacat
    60841 tttgttgaag gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt
    60901 tacctggttt ttagcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta
    60961 gaagttcaaa ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat
    61021 cttttcctca tcgtacctga tgcttttcgc taatcaggct taccgtaggc cgggtaaggc
    61081 gttcacgccg catccggcaa agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc
    61141 cagaatgttc agcagcagga cgcacagggt aatgatcaat accccggccc aggccagttg
    61201 ttgccattcc gcaaacgggc tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat
    61261 cggctgcatc atgtccgtgc tccagaactg gttggagagc gcggtaaaca gcagcggcgc
    61321 ggtttcaccg gcaatacggg caatcgccag caggataccg gtcataatcc cggacaccga
    61381 cgctttcagc gtaatcgcgg agatcatctt ccacttcggt gtacccagcg cataagccgc
    61441 ttcacgcagg ctgtccggca ccagtttcag catgttctcg gtggtgcgga taacgatcgg
    61501 cacctgcaac agcgccagcg caattacgcc cgcccagccg gagaagtgct ccatctgtgc
    61561 caccacgatg gtgtagacaa acagaccaac cacaatcgac ggcgcggaga gcagaatgtc
    61621 gttaatgaag cgaatcactt ctgccagcca ggatttacga ccatattccg ccagataaat
    61681 ccccgccata atgcccagcg gcgtaccgaa taccgtggcc cacaaaatta acagcccgct
    61741 acccgccagg gcgttcgcca gaccaccacc ttccgtattg ggcggcggcg tcatttcagt
    61801 gaacagcgcc agcgacatac cgtcgatacc gcgagtgatg gtggacatta aaatccagat
    61861 cagccagaac aggccgaagg ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt
    61921 gaggcggcga cgcgcctgca ttttgcggcg agattcagcc agcgccgcag tggtttgcat
    61981 ttcaaccata gccattagcg tgccccctca ttcttagcca ggcgcataat cataaacttc
    62041 gatgcggcga ggacgatgaa ggtaatcaca aacaggatca ggcccagttc catcagtgcg
    62101 gcaacgtgca gaccggattc cgcttccgca aattcgttcg ccagcgcaga ggtgatactg
    62161 ttgcccggca tatacagcga ggcgctgtcg agctggtagg tgttaccgat gataaaggtc
    62221 accgccatgg tttcaccgag cgcgcggccc agccccagca tgatgccgcc gataacacca
    62281 tttttggtga acggaagaac gatacgccag ataacttccc aggtggtgca gccaataccg
    62341 taggccgact ctttcatcat caccggggtt tgttcgaaca catcacgcat taccgccgca
    62401 atgtacggaa taatcatgat ggcgaggatc acgcctgccg cgaggatacc gataccaaat
    62461 gcggggccag agaacagcgc gccaacaatc gggatattcg acatgatatt gccgaccggc
    62521 tcctggaagt aaacggcgaa tagcggcgca aagataaaca ggccccacat gccgtaaacg
    62581 atacttggaa tggctgccag cagctcaatg gcgataccca gcgggcgttt cagccagcca
    62641 ggcgcaagct cagtcaggaa cagggcgata ccgaaactca ccgggacggc gatcagcagc
    62701 gcgataaacg aagtcaccag cgtaccgtag atcggcacta gcgccccgta gatatcgttc
    62761 ggtgcatccc actctttggt ccagaggaaa gccagaccaa atttctgaat gctcggccag
    62821 gaggagatga tcagagagac aataatgcca cccaacatca atagcacaat cagcgccgcc
    62881 agttttacca gcacgctgaa aattatgtcg ccctttttac ccggtgggtt aaaagcaggc
    62941 ttggttgcag ccataagtta ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc
    63001 cggataagcc gttaatgcct tatccggcct ggagttttat tagtacagcg gcttaccgct
    63061 actgtcttta atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag
    63121 gctggcgtaa tccaggtcgt tcgcctgttt agcaccggtt ttgtacgccc agtcgaagaa
    63181 tttcagcact tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt
    63241 ggtagaggta ataggccacg catcttcgcc tttctggttg gtcagatcct gagcgaaggt
    63301 tttgctccag tctgcgcctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg
    63361 tttaccatca gcggagatca gtttggtata cgccaggttg ttctgcttcg cgtaagcata
    63421 ttcaacgtaa ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcattacc
    63481 tttaccgccc agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtttttcca
    63541 ctcttcgttc actttcgcca gatagctggt gaagacgaag gaggtaccgg agccatctgc
    63601 gcggcgtacc acggcgatgt tctgtgacgg cagtttcaga cccggattca gtttggcgat
    63661 ggcttcatca tcccacttct tgattttgcc gaggtagatg tcgccgaggg ttttaccatc
    63721 cagcaccagt tcgccagact tcagccctgg aatgttaaca gccagcacca cgccgccaat
    63781 cacggtcggg aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc
    63841 agaggcacca aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat
    63901 accctggtag ttaactttat taccggtttc tttctggtaa gtgtcagccc atttggcata
    63961 caccggcgca gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa
    64021 agcgctcatc gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat
    64081 aatgtctcct gggaggattc ataaagcatt gtttgttggc tacgagaagc aaaataggac
    64141 aaacaggtga cagttatatg taaggaatat gacagtttta tgacagagag ataaagtctt
    64201 cagtctgatt taaataagcg ttgatattca gtcaattaca aacattaata acgaagagat
    64261 gacagaaaaa ttttcattct gtgacagaga aaaagtagcc gaagatgacg gtttgtcaca
    64321 tggagttggc aggatgtttg attaaaaaca taacaggaag aaaaatgccc cgcttacgca
    64381 gggcatccat ttattactca accgtaaccg attttgccag gttacgcggc tggtcaacgt
    64441 cggtgccttt gatcagcgcg acatggtaag ccagcagctg cagcggaacg gtgtagaaga
    64501 tcggtgcaat cacctcttcc acatgcggca tctcgatgat gtgcatgtta tcgctactta
    64561 caaaacccgc atcctgatcg gcgaagacat acaactgacc gccacgcgcg cgaacttctt
    64621 caatgttgga tttcagtttt tccaacagtt cgttgttcgg tgcaacgacg ataaccggca
    64681 tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc gcctgcagcg taggcttcag
    64741 cgtgaatgta agagatctct ttcagtttca atgcgccttc cagcgcgatt gggtactgat
    64801 cgccacggcc caggaacagc gcgtgatgtt tgtcagagaa atcttctgcc agagcttcaa
    64861 tgcgtttgtc ctgagacagc atctgctcaa tacggctcgg cagcgcctgc agaccatgca
    64921 cgatgtcatg ttcaatggag gcatccagac ctttcaggcg agacagcttc gccaccagca
    64981 tcaacagcac agttaactga gtggtgaatg ctttagtgga tgccacgccg atttccgtac
    65041 ccgcgttggt catcagcgcc agatcggatt cgcgcaccag agaagaaccc ggaacgttac
    65101 agattgccag cgaaccaagg taacccagct ctttcgacag acgcaggccc gccagggtat
    65161 ccgcggtttc gccagactgt gacaaggtga tcatcaggct gttacgacgc acggcagatt
    65221 tgcgatagcg gaattcagag gcgatttcga cgtcgcacgg aatacctgcc agcgattcaa
    65281 accagtaacg ggaaaccata ccggagttat aagaagtacc acaggcgagg atctgaatat
    65341 gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag ctcgcttaaa tcaacctgac
    65401 cgtggctgat gcgcccggta agggtgtttt tgatcgcgtt cggctgttcg tagatctctt
    65461 tctgcatgta gtgacggtaa atacctttat cgcccgcgtc atattgcaga ttggattcga
    65521 tatcctgacg ttttacttcc gcgccagttt tatcgaagat gtttaccgaa cggcgagtaa
    65581 tttccgcaat atcgccctct tcaaggaaga taaagcgacg ggtcaccggc aacagcgcca
    65641 gctggtcaga agcgataaag ttttcgccca tacccaggcc aatcaccagc ggactaccag
    65701 aacgtgccgc cagcagggta tccgggtgac gggagtccat gatcactgta ccgtacgcac
    65761 cacgcagttg cgggatagca cgcagaacag cctcacgcag agtaccgccc tgtttcagct
    65821 cccagttcac cagatgggca attacttcgg tgtctgtttc agaaacgaag gtatagccac
    65881 gcgcttttaa ctcttcgcgc agcggttcat ggttttcgat gatgccgtta tgcaccacca
    65941 caatgtgttc agaaacatgc ggatgcgcat tcacttctga aggttcaccg tgggtcgccc
    66001 agcgagtatg agcaataccc gtaccgccat gcagaggatg ttcttccgct gcctgagcca
    66061 gcatctggac tttaccgagg cgacgcaggc gggtcatatg accttccgca tcaacaacgg
    66121 ccagaccggc agagtcatat ccgcggtatt ccagacgacg taaaccttca agaaggattt
    66181 ctgctacatc acgttgcgcg atcgcgccaa caattccaca catagttttt gattccgatt
    66241 tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg cgccccgagc cttgtagaga
    66301 gtggggttat ttttatagtt actgcttgtg ggagggagat tatgttatct cctcatccca
    66361 tgtgaccgga ttacccggcc agaatcactt tttctttacc ggacgacgcc agccttcttt
    66421 ctgagtctgc ggcacacggc tgatagctaa tgcattttcg ccgacattac gcgtcacagt
    66481 tgtacccgca gcaatggtcg cgcctttgcc tactgttacc ggggccacca gctgagtgtc
    66541 ggaaccgaca aacacatcgt cgccgataat ggtcttaaat ttattcgcac catcgtagtt
    66601 gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca atttccgcat cgcccaggta
    66661 agtcagatga ccagctttcg agcctttacc cagacgcgct tttttcatct caacaaagtt
    66721 accgacgtgt gcaccttcca gcaactcagc accaggacgc agtcgggcaa acgggccaat
    66781 agtacaggcc gctgccagat tcgcgtcttc cacaacggta tacggactga tttcgcaatc
    66841 atcgccaatc acgctgtttt taatcacgca accggtgccg attttcacgc gatgaccgag
    66901 agtcacgttg ccctcgatga taacgttagt atcaatttca acatcgcgcc cgtgagtaag
    66961 cgtaccgcgc aaatcaaaac gcgccggatc gcgcagcata acgcctgcta acagcagttt
    67021 ttcagcctgt tcggactggt aaacgcgctc cagacgggag agttgcaggc ggttattcac
    67081 accttctact tcgcttaaac gttgcggatg aacggcgacg atttcacgcc cttcctgata
    67141 cgccagcgca ataatgtcgg tgatgtagta ttcgccctga gcattattgt tggtcagctt
    67201 cgccagccag cgtttcatat ctgcgccgtt agcgatcaga atgccggtgt tgatctcctg
    67261 aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca atgccggtaa ctttgccatt
    67321 ttcacgggtg atacgtccat aaccggtcgg atcatccagt ttcaccgtca gcagaccaat
    67381 gccaccctgc ggtttagcat cacgcaggcg ctggagtgtt tcgacagaga tcagcggcac
    67441 gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag aaaggtgcgg cctgctgcat
    67501 tgcatgaccc gtacccagct gctctgcctg aagcacccag ttaaggttgt cgtctttcag
    67561 cgcctgttta agcagatcgc cgccgtgacc gtacaccagg tgaacgtgcg ctgcgcctaa
    67621 ttcattcgca gcatcaatga catgctgaac catcgctttc ccggcaaggg tatgcagcac
    67681 tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg gcaaggatca ctacgctcat
    67741 agcattattc aacatacgcg tcctgactgt aatttgagaa cgaatttaaa ccgcttcacc
    67801 ttgaaaaaac tacatttttt tcatcgtgaa atggacagag gataaattgt tcaataacgg
    67861 attatccccg ctgacgaaag tgccattttc gaccatcgtc acgccttttg tctgcagaaa
    67921 attaaggcag taccttcatt taagcgtcag gtggatgttt ttgctcttat ttcgatcaat
    67981 gaataaacgg gaaaacaggg gtgttttata tcagcaggat ctatgtgaac gctcttcagg
    68041 acggatcacg cgtaaaaaaa agccagcctg tttccagact ggcttttgtg cttttcaagc
    68101 cggtgttaca tcgctttttt ggtcaactcg ataacgcgca gctgcgcgat cgctttggcc
    68161 agttccgcag acgcctgagc gtaatctacg tcgccgtgag agctgctaat gtgctcttca
    68221 gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat cctggccgcg aattgcggtg
    68281 tcggccagaa cggtcacgtt gccaggctgc acttcaagaa tgccgccaga cagatagata
    68341 aactcttcgt gaccgtgctg tttcacgatg cgaatcatac caggcttaat ggcggtgagc
    68401 agcggtgcgt ggccagggta gatccccagt tcaccttcgc tacccgttac ctggattttc
    68461 tcgaccagac cagagaacat ttgttgctct gcgctgacga cgtccaggtg gtaagtcatt
    68521 gccatatcac cctccgatta aggcgttaaa gttttttggc tttttccaca gcttcttcga
    68581 tggaaccgac catgtagaac gcctgctccg gcaggtgatc gtattcgcct tccatgatgc
    68641 ctttaaagcc acggatggtg tctttcaggg agacgtattt acccggagaa ccggtgaata
    68701 cttctgccac gaagaacggc tgggacagga agcgctggat cttacgagca cgcgctacca
    68761 ccagtttgtc ttcttcagac agttcatcca tacccaggat ggcgatgatg tctttcagtt
    68821 cctgataacg ttgcaggatg gactgaacgc cacgcgcggt gtcgtagtgt tcctgaccaa
    68881 ccaccagcgg gtccagctga cggctggtgg agtccagcgg gtcaacggcc gggtagatac
    68941 ccagagacgc gatctgacgg ctcagtacca cggttgcgtc aaggtgcgca aaggtggttg
    69001 ccggagacgg gtcagtcaag tcatccgcag gtacgtatac tgcctgtacg gaggtgatag
    69061 aaccagtttt ggtggaggtg atacgttcct gcagaacgcc catctcttcc gccagggtcg
    69121 gctgataacc taccgctgaa ggcatacggc ccagcagtgc ggatacttcc gtacctgcca
    69181 gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc acgaccttcg tcacggaatt
    69241 tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg gtttcccggc ggctcgttca
    69301 tctggccata caccagggat actttgtcga taacgttgga gtcggtcatt tcgtggtaga
    69361 agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa cacagagtaa ccggagtgct
    69421 cgatcgcgat gttacgaatg agctccatca tgtttacggt tttacctaca cccgcaccac
    69481 cgaacagacc aactttaccg cccttagcga acggacacat caggtcgata actttgatac
    69541 cggtttccag cagttcctga gagtttgaca gctcttcgta ggaaggtgct gcgcggtgaa
    69601 tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc gaccggttca cccagtacgt
    69661 tcatgatacg gcccagagtc gctttaccta ccgggacttc gatcgggtgt tcgaggtctt
    69721 ttacatccag accgcgacgc agaccgtcgg aggaacccat tgcgatggta cgcacgatac
    69781 cgccgccgag ctgctgctga acttccagca ccagacgctc attaccattt tgcacctcaa
    69841 gagcatcgta cacgcgcggt acggcatcct gagggaattc gacgtcaact acggcgccga
    69901 ttacctggac aatctttcca gtagccatct taaatcctct acgaaataac ctgtttaaac
    69961 cgcggcggcc cccgagacga tctcggtgag ttcctgagta atgctggcct gacgagcttt
    70021 gttgtatacc aactgcagct ctttaatcag gctgccgcca ttgtcggtcg cggctttcat
    70081 cgccaccata cgggcggcct gctcgctggc caggttttca accacgccct gataaacctg
    70141 agattcgaca taacgacgca gcagggtatc cagcaacgcc ttcggatcgg gttcgtacag
    70201 gtaatcccag gatttatgtt tcagatcatc atcatctgat gccggtaacg gcagcagctg
    70261 gctgatggtc ggaacctgag acatggtgtt aataaatttg ttgctgacaa tgtaaagctt
    70321 gtccagacgg ccttcgtcgt aggcctgcaa catcactttt accggaccga tcagttcgga
    70381 cagggaaggg ttatccccca tgccggtgac ctgggcaaca acattgccgc ccacggagtt
    70441 gaagaacgac acgcctttcg agccgatcat tgcgaggtcg cattgaacgc ctttgtcggt
    70501 ccaggtcttc atttccgcca gcagtttttt gaacaggtta atgttcaaac caccgcacaa
    70561 accacggtcg gtcgacacca ccaggtagcc cacgcgttta acgtcgcggt cttccaggta
    70621 agggtgctta tattccagat taccgtgtgc aaggtgacca atcactttgc gcatggtttc
    70681 tgcataagga cggctggccg ccatgcgatc ctgcgattta cgcattttgg aagcggcgac
    70741 catctccatc gctttagtga tcttttgcgt gttctggacg cttgcgatct tactacgtat
    70801 ctcttttgcg ccggccatga gcttctcctc aatgccttgc ggcctgccct aaggcaagcc
    70861 gccagacgtt accaggattg ggttgctttg aaggaatcga ggatgccttt cagcttgcct
    70921 tcgatttcgt cgttgtagcc accggtctgg ttgatctctt gcatcaacgg agcgtgatca
    70981 cggtcgacgt aagccagcag agcggcttcg aagctgccaa ttttcgacag ttcaacatcc
    71041 gccaggtaac cacgttctgc tgcgaacaga accagagact gctgcgcaac ggacatcggc
    71101 gcatactgtt tctgtttcag cagttcggtc actttctgac cgtggtcaag ctgcttacgt
    71161 gttgcatcgt caaggtcgga tgcaaactga gagaacgctg ccagttcacg atactgtgcc
    71221 agagcggtac ggataccacc ggacagtttt ttcatgatct tggtctgtgc tgcaccacca
    71281 acacgggata cggaaatacc cgggttaacc gcaggacgaa taccggcgtt gaacaggttg
    71341 gtttccagga agatctgacc atcggtaatg gagattacgt tggtcggaac gaacgcagaa
    71401 acgtcacccg cctgagtttc gataatcggc agtgcggtca gagaaccggt tttccctttc
    71461 acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa cacgtgcagc acgctccagc
    71521 agacgagagt ggaggtagaa aacgtcgccc gggaatgctt cacgtcctgg cggacgacgg
    71581 agcagcaggg agatctgacg gtaagcaaca gcctgtttag acaggtcatc gtaaatgatc
    71641 agcgcatctt caccgcggtc acggaagtat tcgcccattg cgcaaccggc atacggtgcc
    71701 aggtattgca gtgcagcgga ttcagacgcg gttgctacca caacgatggt gttagccagt
    71761 gcgccgtgct cttccagttt acgtaccacg ttagaaatgg tggacgcttt ctggccgata
    71821 gcgacataga tacatttgat accggaatcg cgctggttga tgatagcatc gatagccagt
    71881 gcggttttac ctgtctgacg gtcaccgatg atcaattcac gctgaccacg accgattggg
    71941 atcatggagt caacggcttt ataaccggtc tgtaccggct gatctacgga ctgacgttcg
    72001 ataacgcccg gagcgattgc ttctacagca gagaagccgt cgtgatccag cggaccttta
    72061 ccgtcgattg gtgcacccag agtgttaacc acacggccca gcaggccacg gccaaccgga
    72121 acttccagga tacggccagt acacttaact ttcatgcctt cggcaaggtc agcgtacgga
    72181 cccataacaa ccgcacctac agagtcgcgc tcgaggttca gtgcgatagc gtaacggtta
    72241 cccggcaggg agatcatttc accctgcata caatcggcca ggccgtgaat gcggataaca
    72301 ccgtcactta cagaaacaat agtaccttcg ttgtgagctt cactcacaac attgaactga
    72361 gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat tcagttgcat gctccagtcc
    72421 ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac cgcgtacgct gccatcaatg
    72481 accatatcac ccgctcggat gataacgcct gccattacag acttatcgat tttgcaattc
    72541 agcttaactt tgcgtgacag acgtttttcc atcgcagcag aaattttcgc gagctgttgt
    72601 tcactcagtg cggcagcgga aatgacgtct acctcagcgg tagcctcact cacggcacga
    72661 aggtgaataa actgctccag aacatccggg agcgcgttaa gacgaccatt ttcagccatt
    72721 acccgaatca ggttctgacc gttttcgtcc agttgctcac cacaaactgc gataaacgac
    72781 tcggcgagcg tttctggcgc aagcgcgcca gagagaagct ctgccatttg ttcgtttttg
    72841 gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt ctacactttg gtgttcgacg
    72901 gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg taataaattc agacatcagc
    72961 ccctccctcc ttacagttca gcgacaagtt tatccacgat gtcgctgtta gcagcttcat
    73021 ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc caggatagca acttgcttac
    73081 gcagctcttc acgggcacgt ttacgctcgg cttcaatttc tgcctgcgcc tgggccacga
    73141 ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc cagaatctgc gagcggcgtt
    73201 tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc ttttttcagc tggtcggtcg
    73261 cgctggcctt tgcaaggtca aggtccttat gtgctcgttc tgcggaagca aggccgtcag
    73321 caatttcttt ttgacgtttt tcgatggctg ccattaatgg cggccatacg tacttcatgc
    73381 agaacagaac gaacaggaca aacgcgatgg cctggccgag gattgttgcg ttaagattca
    73441 cagcacaatg cctctattta gttaacgttc tgatattgct ctttaaataa aagcaacgct
    73501 tactacgcga cagcgaacat cacgtacaga cccagaccta cagcgatcat cgggatagca
    73561 tccaccagac ccataacgat aaagaactga gtacgcagca gaggaatcag atcaggttga
    73621 cgcgctgcgc cttccaggaa tttacccccg aggatgccga taccgatcgc agcaccgatt
    73681 gccgccagcc ccatcatcac agcggcagcc atgtacagca gatccatatt caggttttcc
    73741 atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg ttggtaaatt aatgttcttc
    73801 agacgccatc gacagataga cgatcgtcag aaccatgaag atgaaggctt gcagcgtaat
    73861 gatcaggatg tggaaaatgg cccacggcac attcaggatc cactgtgacc accacggcaa
    73921 cagaccagca atcagaatga aaatcagctc accggcatac atgttaccga acagtcgcaa
    73981 accaagtgaa actggtttgg acagcaggct taccccttca aggattaagt tgacaggaat
    74041 gaacgcccag tgattgaacg gctgcagcgt caactctttc gtgaagccgc cgatgccttt
    74101 cattttgatg ctgtagaaca gaatcaggat aaatacgccc aatgccatag acagcgttac
    74161 gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga cccagtacat gttcagcaat
    74221 gtacggcagc aggtcgatag gcagtaaatc catcaggttc atcaggaata cccagacgaa
    74281 gatcgtcagg gccagcggag caatcagctt gcttttgcca tggtacatgt ctttcacgct
    74341 accattaaca aagccgatca ccagctcaat cgcggtctga aacttacctg gcacaccgct
    74401 ggtcgccttt ttggctacgc tacggaataa aaccaggaac aacagaccca gcaccaccga
    74461 gaagaacatg gagtcaatat tgattgtcca gaaggtggct ggggggtttt gtggatccac
    74521 cagcgagaat gtacgcaggt ccagctgaag gttattcagg tggtgtccta tgtaatcctg
    74581 cggcgtcata ttttctgaag ccatgatgcc ttttaccctt tgttgttaat tacagccggt
    74641 gccagtatct gaaccaccag caccaaaacc cacgtaacga tcagcggcaa gaataccgcc
    74701 tttaaaaccg ccaacgccac caccagtaac accaacatcg ccagaacttt gaaagcttcg
    74761 ccaaatgcga atgtccaggc cacccggcct ttcgctggtg tatgcgcctg gtgacgccag
    74821 gcaaatatca taaacaaaac gttaggcaga aagactgcca ggcccccgct tattgcagag
    74881 acgccccaga aggggtcttt gaggctgaac agcaatccac ttgctatcac caccagtaac
    74941 tgaacgagca gaagcttccg agcaacgttt cgactcacga gcgacacaga catcacgttt
    75001 ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata aaactttctt taaggcttag
    75061 agtcaagcat caaaaagcgg tcaaattata cggtgcgccc ccgtgatttc aaacaataag
    75121 tagccaaaag gtgaataaat gtttaaatat ttttccagtg catacaattg cgacttttct
    75181 gctaaccctg ttcgatcatg aaaaactgta aataacgcgt aaacactggt gataaagcgt
    75241 gcttcagatc acatattgcg catgttcgcg cacagcatat ttatttactt ggcaaatgat
    75301 gcctttgcaa gtttatgata tttaagtcta aaaacagata ctgttttaat aaatgacatt
    75361 tacacaacaa aaaccaccca ttgacatttt taataatgtt ttaacagcca atgatgattc
    75421 ttagcgccga tttttagcag actgatattt tcactaatga cttattttct gcttaccaaa
    75481 aaaagccacg ttatcttgtt gatgcaaaag agtgaacgtg gcgttaaatg taaccagtta
    75541 tatcagtaga aaacctggtt gttgttaaca gtctaaccgg tcaatttttt atgatttttt
    75601 tgataaaaat taaattttat ttgctttaat caccaccaga tggcgttcgc catccagggc
    75661 aggaacctga agtttaacca ctgattcaac ctgatattct tcgggcaaca aagcgatttc
    75721 atcttccggc atttgccctt tcagcgcgta gaaacggcct tgctcaccag gaagatggtg
    75781 acaccagctc accatatcgt tcagagaggc aaaagcgcgg ctaattacgc catcaaatgg
    75841 cggctctgaa ggaaactctt ctaccctgct ctgtactggc tcaatattct ccagtttaag
    75901 ctcatgttgc acctgacgaa ggaaacgcac gcgtttacca aggctatcca acagagtgaa
    75961 atgggcttca ggacgcacga tagagagtgg aatgccaggc agtccaggac cggtgccgac
    76021 atcgataaac cgttcacctt gcagatacgg tgccaccaca atgctatcga gaatatggcg
    76081 taccagcatc tcattaggat cgcggaccga agtcaggttg tacgctttgt tccatttatg
    76141 cagcatattc acgtaggcaa taagctggtt tttctggtga tcggtaagcg aaatacctgc
    76201 gtctttcagc agtaaggaga gtttgttgag cacggtgatt acctgttctt gatgcgttgc
    76261 ctggtaagcg ggtgcttacc aggcattttt aatgcgttat gcgctacgac gcagcatacc
    76321 ctgttttttc agccacacca gcagaatgga gatggccgca ggcgtgacgc cagaaatacg
    76381 tgaagcctgg ccgatagagg ccggtttgtg atcgttaagt ttggcgatca cttcgttaga
    76441 aagaccggat acctggcggt aatccagtgt cgctggtagc agggtgttct cgttacgcag
    76501 ctgcttttcg atctcatctt gctggcgcgc gatataacct tcgtatttaa cctgaatctc
    76561 aacctgttcc gccgcctgtt cgtctgtcaa cgcaggggca aacggcgtca gcgtggttaa
    76621 tttttcataa gtcatttccg gacgacgcag cagatcttca ccactggctt cacgggaaag
    76681 cggcgcagtc aggtgagcat tcacttcggc tgcagcttcc gccgacgggg ttacccaggt
    76741 cgatttcaga cgctgacgct cacgctcgat attctcaagt ttctcgttaa agcgcgccca
    76801 acgttcgtca tccaccaggc ccagttcacg accgatttca gtcaaacgca gatccgcatt
    76861 atcttcgcgc agcatcagac gatattctgc gcgcgaagta aacatacgat acggttcttt
    76921 ggttcctaaa gtgcacaggt catcaaccag tacgccgaga taggcctgag aacgcgccgg
    76981 agcccaacct tctttgtcag cagacagacg ggcagcgtta agaccggcca gcaaaccttg
    77041 cgcagcggct tcttcgtaac cggtagtgcc gttaatctga ccagcaaaga acagcccctg
    77101 gataaacttg ctctccagcg tcggtttcag gtcgcgagga tcgaagaagt catactcaat
    77161 ggcataaccc ggacgcacga tcttcgcgtt ttccatcccc tgcatagagc ggacgatttg
    77221 catctgcaca tcgaacggca ggctggtgga gataccgttc ggataaattt cgttagaagt
    77281 cagcccttcc ggttcaagga agatctgatg ctgatttctg tcggcgaagc gcatgacttt
    77341 gtcttcgatc gacgggcagt agcgtgggcc gacaccttcg atcacccctg catacattgg
    77401 gctacgatcg aggttactgc ggatcacatc atgggttttc tcgttggtat gagtgatata
    77461 acacggcacc tgctggggat gctgggacgc attgcccata aacgagaata ccggcattgg
    77521 gttatcgcca tgctgttgcg ccagcacact aaagtcgata gttcgagcat caatacgcgg
    77581 tggtgtcccg gttttcagac gaccaacgcg cagcggcagt tcacgcaaac ggcgagaaag
    77641 cggaatggac ggcggatcac cagcacggcc accgctgtaa ttatccagac cgatatgaat
    77701 tttaccgtcg aggaacgtcc caacggtgag cacgacggct ttggcacgga acttcagtcc
    77761 catttgggta acagcaccga ccacgcgatc gttttcgaca ataagatctt caaccgcctg
    77821 ctggaagatc atcaggttcg gttggttctc cagcgccgta cgtaccgcct gacggtagag
    77881 cacacgatcc gcctgagctc gggtagcgcg aaccgccggt cctttgcttg cgtttagtat
    77941 cctaaactgg atacccgctt gatcgatcgc tttcgccatc agaccgccga gtgcatccac
    78001 ttcttttacc agatgtccct tcccaatacc gccgatcgcc gggttgcagc tcatctgccc
    78061 cagagtgtcg atattgtgtg tcaaaagcag agtctgttga cccatacgcg ccgcggccat
    78121 cgcggcctcg gtgcctgcat gacccccgcc aatgatgatg acgtcaaaag gatccggata
    78181 aaacatggtg attgcctcgc ataacgcggt atgaaaatgg attgaagccc gggccgtgga
    78241 ttctactcaa ctttgtcggc ttgagaaaga cctgggatcc tgggtattaa aaagaagatc
    78301 tatttattta gagatctgtt ctattgtgat ctcttattag gatcgcactg ccctgtggat
    78361 aacaaggatc cggcttttaa gatcaacaac ctggaaagga tcattaactg tgaatgatcg
    78421 gtgatcctgg accgtataag ctgggatcag aatgaggggt tatacacaac tcaaaaactg
    78481 aacaaccgtt gttccttgga taactaccgg ttgatccaag cttcctgaca gagttatcca
    78541 cagtagatcg cacgatctgt atacttattt gagtaaatta atccacgatc ccagccattc
    78601 ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg atcttcagtg tttcgcctgt
    78661 ctgttttgca ccggaatttt tgagttctgc ctcgagttta tcgatagccc cacaaaaggt
    78721 gtcatattca cgactaccaa taccgattgc gccaaagcgg accgcagaaa gatcgggctt
    78781 ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc ggaatatctc cggcaccgtg
    78841 ggtggagctg ataatcagcc agatccccga ggcagataaa tcttccaaca gcggaccgtg
    78901 cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca gccaggtgtt ctgctacata
    78961 ttcggcaccg ccgagggtgc tgccgctgat aagagtgata tctgccataa accgccacct
    79021 ttattaagag tggcgtattg tacgctgtga acgcgttggg atctacctgt ggaaaagtat
    79081 gggattaaaa aagccgatca gggcttgata gtacgcatga tcgggttttg caggacgatc
    79141 aatgtctcgg tggactgaat ttcatcaatt gtttggatct tgttgataag tacatgctgg
    79201 agagcgtcga tcgaacggca cattactttt ataaagatgc tgtagtggcc ggttgtgtaa
    79261 taggcttcag tgacttcatc aaggctttcc agctttgcca gcgcggaagg gtagtctttg
    79321 gcgctcttta atataatgcc gataaagcag cctacgtcat aaccgagctg cttcgggctg
    79381 acatcaatac gcgccccggt aatgatcccc gcctgcttca ttttctctac tcgaacgtga
    79441 atcgtccccg gactgacgcc aaattgtttc gccagttcgg cgtaagcggt gcgcgcattg
    79501 cccattaatg cttccaggat gccacggtcc agattgtcga tcagataatt ttccatagga
    79561 ttttcttatg cagattgatg attcattcta ttttagcctt cttttttaat gaatcaaaag
    79621 tgtgttaggc tttttattga atgattattg catgtgtgtc ggtttttgtt gcttaatcat
    79681 aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt acattgccaa acaacgccaa
    79741 attagcttcg tgaaatctca cttttctcgc caactggaag aacgtctggg gctgatcgaa
    79801 gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc aggataactt gtcgggctgt
    79861 gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg cccagttcga agtggttcat
    79921 tcactggcga agtggaaacg tcagacctta gggcaacacg acttcagcgc gggcgaaggg
    79981 ctgtacacgc acatgaaagc ccttcgcccc gatgaagagc gtctttctcc gttgcactcg
    80041 gtctatgttg accagtggga ctgggaacgc gtaatgggcg acggtgagcg tcaattctcg
    80101 actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga agctgcagtt
    80161 agcgaagagt ttggtttggc accgttcctg ccggatcaga tccacttcgt acacagccag
    80221 gagttactgt ctcgttatcc ggatctcgat gccaaagggc gtgagcgggc gattgcgaaa
    80281 gatcttggcg cggtattcct cgtcgggatt ggcggcaagc tgagtgatgg tcatcgtcac
    80341 gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg ccatgcgggt
    80401 ctgaacggcg atattctggt atggaaccct gtactggaag atgcgtttga gctttcttcc
    80461 atggggatcc gcgtggatgc cgacacgttg aagcatcagt tggcgctgac cggtgatgaa
    80521 gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg gtgaaatgcc gcagaccatc
    80581 ggcggcggta tcggccagtc tcgtttgacc atgctgctgc tgcaactgcc gcatatcggc
    80641 caggttcagt gtggagtatg gccagctgct gttcgcgaga gcgtcccttc tctgctgtaa
    80701 taatttatcg ccgccagcgt ctgagcaggg ggcttcgcat cccggtatca aagcgccaga
    80761 tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc cgacatcgcc acggcatgaa
    80821 agcgatgctg atgtacccgc tgcagctctt tcactttact cgtcacgtcg tcaggcaacc
    80881 gctgagcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc ctgctttgca
    80941 agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga aactgctggc
    81001 ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa cggacgatct
    81061 cggtggaaaa tagcataata tagcagcgcc ggttttctgc gagagcaatg cgcatcaagg
    81121 ccaggcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg gaagtatcca
    81181 cacagacaat aaacggcccg cgcggctgtt catcgtaatc tttatgtacc accggacgtt
    81241 cgatcacttt ttcacgccac gactcaccgt gcaggcgata ggtgagcaac tgtttttcca
    81301 ccagccgacg gtaaaactca tactccagtt ccgttatccc tagtgtcgcc agttctggcg
    81361 gcagaagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca ggaaccgtcg
    81421 ccggttcgcg caccatggtg cggaaggttt ccatctgcgc atcgttgcgc ggtattgatt
    81481 tggcttcccg agaacgcccc agctgctctg ccaggcgttt cagttccggc tgttcgttaa
    81541 gaaattcacc gtatttcaca atcaactgat agtcgccacg tttaagctga ccggcgctca
    81601 tatcccacag acgaccagct gcagtattgt tatctgcgag aatcggttca agttgtccgc
    81661 tcagcgtcat gcgttcctga acttcactca acagttgttc gcgttcttct tctaatagct
    81721 gttgatttaa cgtcgttgct tgcacgatca gacttaaacg ccaacgctgg agaaaaagcg
    81781 tgtgtaacgc tgaagtgatc gtgctgttag catcaaccaa ctgtcgggct tgttctgccc
    81841 atggagaatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca ataaactgtg
    81901 gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt tccggcggga
    81961 ctcgggcatc tttcagccga ctgcgcagcg cttcacgcca gcggggaaca tcatcagtga
    82021 ttgccgcctt caggcgtggg aatttttcaa agaagaccgc cagctgcggt gaggccagca
    82081 gtgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc acattaagcg
    82141 tatccagcgt tagcattgtt gtgcctggcg aatctgtgca ccgacatcct gtaggctggc
    82201 ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt cgctgaataa
    82261 agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt tgttgtttta tttcaccagg
    82321 cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac ttacatcgcg
    82381 aacgacaaga tgttgggcgc tatcaatttc cagattcagt ttctgggcaa agccgatacc
    82441 gttcagttta ccgcgaattt caccaccttt gctcagccac tgttccagcg cgctacgctc
    82501 aaaggagata tgaaccactt ccatatcatg cagttttaac ggtttttgca gcagtagagt
    82561 cagagtggaa gcagtaacgt taacaggaag ttgatactgc tgacgacggc tgaaaatgcc
    82621 gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgga gttgcaggtg
    82681 acgttgcaca atcgcgccaa ggcgagtcaa catcccttgc tgttgccagg cgtgaccggt
    82741 catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat accacaggca
    82801 atctttcagc aaaatgagat ctaccggggc aacagcactg cgaccgctaa aaaaggcgct
    82861 ggcctgcaat aatcggatcg cttttttcca gcgacgatcc gagacataag gcgcatccgg
    82921 taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat cgggcagcgt
    82981 aatttcacca atctctttct gccagcgttc atattcttca tctgtgacct gcaaggcatc
    83041 aggaaccgga ttgtcgtttt catcctgttg actggtcagc atggagcgga aattcgcttt
    83101 atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg cttccagact
    83161 gctgtctgct tccggcagct cgttggaggc cgccaccagc agacgcatcg ggattttttc
    83221 tacgtgtgca ccgttgcgga actggcgctc gttaatggcg gtgagcaagg tattaagaat
    83281 tgccgggccc gctttccaga tctcatccag aaagacaatt tctgcttccg gcaggtaacc
    83341 gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa ggggaccaaa
    83401 aacttcttcc ggcgtggaga agcgggtcat cagatattca aacgcgcggg cattctgaaa
    83461 ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggcgggc caaggaggaa
    83521 cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc tacgttcata
    83581 aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata aatgagggtg
    83641 agccataatg aagtggcgtc ctttcgtcaa aagttctgcg taaattgcga gtatagacgt
    83701 ttcttgctgg tggctaaaat agtctcaaag gggggggatt tttctttgag ccaggttaat
    83761 gtggccgcat ttaggagtac gattttgccg ttaatcgtgc atactgtgcg cttttttgtg
    83821 ggccaaggga ctaagcacac atttcatatt tcaacgaaag actagtctat gagcactgat
    83881 aataagcaat cattgcccgc gattaccctc gcggcgattg gagttgtcta cggcgatatt
    83941 ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg gccagtttgg ttttggcgtt
    84001 gaacgcgatg ccgtgtttgg ctttttatcg ctgatcttct ggctgctaat ctttgtggtt
    84061 tccattaaat atctcacctt cgtgatgcgg gcagataacg ccggtgaggg ggggatcctg
    84121 acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa ccacatcaat gctggtgatt
    84181 atggggctaa tcggcggcag ctttttctat ggtgaagtcg tcataacacc cgctatttcg
    84241 gtgatgtcag ccattgaagg tctggaaatc gtcgccccgc agctggatac ctggatagtt
    84301 cccctctcaa ttatcgttct cactttatta tttatgattc aaaaacatgg cacggctatg
    84361 gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt tgattctggc aggactgggg
    84421 ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc tgaatccaat gtgggcggtg
    84481 catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt ggtgttgtcg
    84541 attacggggg gcgaggcgct gtatgctgat atggggcact ttggtaagtt ccctattcgc
    84601 ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta attacttcgg tcagggagcg
    84661 ctgttgttaa agaacccgga agcgattaag aacccgttct tcctgttggc accggactgg
    84721 gcgctgatcc cgctgctgat catcgccgca ctggcgacgg taattgcctc gcaggcggtt
    84781 atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt gtcgccgatg
    84841 cgcattattc acacctccga aatggagtca gggcaaatct atattccgtt tgtgaactgg
    84901 atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag caacctggcg
    84961 gcggcgtacg ggattgcggt gaccggaacc atggtgctga cgtctattct ctcgactacc
    85021 gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct gattgctttc
    85081 ctttgtgtcg atattccatt gttcaccgct aacctcgata aactgctctc cggcggctgg
    85141 ttgccattga gcctcggtac tgtgatgttt atcgtgatga ccacctggaa gagcgagcgt
    85201 ttccgcttac tgcggcggat gcatgaacat ggtaactctc tggaagcgat gattgcttcg
    85261 ctggagaaat caccgcctgt tcgcgtgccc gggaccgcgg tgtatatgtc gcgtgcaatc
    85321 aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt gcatgagcgg
    85381 gtgattctgt taactctgcg taccgaagac gcgccatatg tccataatgt ccgtcgggta
    85441 cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg ttggcgagaa
    85501 acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag ttgccggatg
    85561 atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg cccgtggtat
    85621 ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg tgcaccagat
    85681 caatttgaaa ttccgccaaa cagggttatc gaactgggta ctcaggtcga aatctaacgc
    85741 cagacgcctc ctttctccat aagggggcgt ttttgttttc atggttaatc accatgtaaa
    85801 acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac tcagtcagga
    85861 ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa aaggcaccgt
    85921 tcttaattct gatatttcat cggtgatctc ccgtctggga cataccgata cgctggtggt
    85981 gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt atcgatatgg cattaaccca
    86041 gggtgtacct tcttttatgc aggtgctggg cgtcgtcaca aatgaaatgc aggtcgaggc
    86101 tgccattatc gcggaagaga tcaaacaaca taatccgcaa ctccacgaaa cgttgctcac
    86161 tcaccttgag cagctgcaaa aacaccaggg aaataccatt gaaattcgtt acaccacgca
    86221 tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg gagaatgttc
    86281 tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc atggaagcat
    86341 tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc tcgggcgcag
    86401 cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaaacggc gcgggtaaat
    86461 ccaccatgat gaaagtgctt actggtatct atgctcgcga tgccggcacg cttttatggc
    86521 tggggaaaga aacgacattt accgggccga aatcttccca ggaagccggg attgggatta
    86581 tccatcagga actgaacctg atcccgcagt tgaccattgc cgaaaacatt ttcctcggtc
    86641 gtgagtttgt taatcgcttt ggcaaaattg actggaaaac catgtatgcc gaagcggata
    86701 aattgctggc taaacttaac ctgcgcttta aaagcgacaa gctggtgggc gatctttcca
    86761 tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag ctttgagtcg aaagtcatca
    86821 ttatggatga accgaccgat gcgctgaccg ataccgaaac cgaatccctg ttccgcgtca
    86881 tacgcgagct gaaatcgcaa ggccgcggta ttgtctatat ctcccaccgc atgaaagaaa
    86941 tcttcgagat ttgcgatgac gttaccgttt ttcgtgacgg gcaatttatt gctgagcgcg
    87001 aagtggcatc actgaccgaa gattcgctga ttgagatgat ggtgggccgc aagctggaag
    87061 atcaatatcc acacctggac aaagcgccgg gagatatccg cctgaaagtc gataatctct
    87121 gcggacttgg cgttaacgat gtctctttta ctttacgcaa aggcgaaatt cttggcgtct
    87181 ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt gctctacggc gcactaccgc
    87241 gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt tacccgttca ccgcaggatg
    87301 gcctggcaag cggcattgtg tatatctccg aagaccgtaa acgtgacggt ttagtgttgg
    87361 gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg ctacttcagc cgcgctggcg
    87421 gcagtttgaa gcatgccgat gaacagcagg ctgtgagtga tttcattcgt ctgtttaatg
    87481 tgaaaacacc gtcgatggaa caggcaattg gtctgctttc cggtggcaat cagcaaaaag
    87541 tggcgattgc ccgcggtctg atgacacgcc ccaaagtgtt gatccttgat gaacctaccc
    87601 gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact gattaaccag ttcaaagccg
    87661 atggcttgag catcattctg gtgtcatcgg agatgccaga agtattaggc atgagcgatc
    87721 gcatcatcgt catgcatgaa gggcatctca gcggggaatt tactcgtgag caggccaccc
    87781 aggaagtgtt aatggctgcc gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa
    87841 atgacaaccc agactgtctc tggtcgccgt tatttcacga aagcgtggct gatggagcag
    87901 aaatcgctta tcgctctgct ggtgctgatc gcgattgtct cgacgttaag cccgaacttt
    87961 ttcaccatca ataacttatt caatattctc cagcaaacct cggtgaacgc cattatggcg
    88021 gtcgggatga cgctggtgat cctgacgtcg ggcatcgact tgtctgtagg ttctctgttg
    88081 gcgctaaccg gcgcagttgc tgcatctatc gtcggcattg aagtcaatgc gctggtggct
    88141 gtcgctgctg ctctcgcgtt aggtgccgca attggtgcgg taaccggggt gattgtagcg
    88201 aaaggtcgcg ttcaggcgtt tatcgctacg ctggttatga tgcttttact gcgcggcgtg
    88261 accatggttt ataccaacgg tagcccagtg aataccggct ttactgagaa cgccgatctg
    88321 tttggctggt ttggtattgg tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg
    88381 attgtcttcc tcgcggcctg gtacatgctg catcacacgc gtctggggcg ttacatctac
    88441 gcgctgggcg gcaacgaagc agcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa
    88501 atcatcgtct attctctttg tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg
    88561 cgtctctcct ccgcacaacc cacggcgggg actggctatg agctggatgc tattgctgcg
    88621 gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc
    88681 ggcgcattaa ttcttggctt ccttaataat ggattgaatt tgttaggtgt ttcctcctat
    88741 taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag
    88801 cagtaataac gactacagga catcttgaat atgaacatga aaaaactggc taccctggtt
    88861 tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg
    88921 ctggtggtct ccacgcttaa caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa
    88981 gaggcggata agcttggcta taacctggtg gtgctggact cccagaacaa cccggcgaaa
    89041 gagctggcga acgtgcagga cttaaccgtt cgcggcacca aaattctgct gattaacccg
    89101 accgactccg acgcagtagg taatgctgtg aagatggcta accaggcgaa catcccggtt
    89161 atcactcttg accgccaggc aacgaaaggt gaagtggtga gccacattgc ttctgataac
    89221 gtactgggtg gcaaaatcgc cggtgattac atcgcgaaga aagcgggtga aggtgccaaa
    89281 gttatcgagc tgcaaggcat tgctggtaca tccgcagccc gtgaacgtgg cgaaggcttc
    89341 cagcaggccg ttgctgctca caagtttaat gttcttgcca gccagccagc agattttgat
    89401 cgcactaaag gtttgaacgt aatgcagaac ctgttgaccg ctcatccgga tgttcaggct
    89461 gtattcgcgc agaatgatga aatggcgctg ggcgcgctgc gcgcactgca aactgccggt
    89521 aaatcggatg tgatggtcgt cggatttgac ggtacaccgg atggcgaaaa agcggtgaat
    89581 gatggcaaac tagcagcgac tatcgctcag ctacccgatc agattggcgc gaaaggcgtc
    89641 gaaaccgcag ataaagtgct gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg
    89701 aaactggttg ttaagcagta gttttaatca ggttgtatga cctgatggtg acataaatac
    89761 gtcatcgaca gatgaacgtg taatataaag aaaagcaggg cacgcgccac cctaacacgg
    89821 tggcgcattt tatggacatc ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca
    89881 gcattaatgc tgaccacatt cttaatcttc aatcttttcc tactccaggc gaaaccgtaa
    89941 ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg
    90001 ggcgtagcgg tgcgaatatc gcgtttattg cctgtacggg tgatgacagc attggtgaga
    90061 gcgttcgcca gcagctcgcc actgataaca ttgatattac tccggtcagc gtgatcaaag
    90121 gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga aggtgagaat gtcatcggta
    90181 ttcatgccgg cgctaatgct gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta
    90241 ttgccaacgc gtcagcatta ttaatgcagc tggaatcacc actcgaaagt gtgatggcag
    90301 cggcgaaaat cgcccatcaa aataagacta tcgttgcgct taacccggct ccggctcgcg
    90361 aacttcctga cgaactgctg gcgctggtgg acattattac gccaaacgaa acggaagcag
    90421 aaaagctcac cggtattcgt gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac
    90481 tgcatgaaaa aggtatccgt actgtactga ttactttagg aagtcgtggt gtatgggcta
    90541 gcatgaatgg tgaaggtcag cgcgttcctg gattccgggt gcaggctgtc gataccattg
    90601 ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc
    90661 cagaggcgat tcggtttgcc catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac
    90721 aaccttccgt accgtggcgt gaagagatcg acgcattttt agacaggcag aggtgacgct
    90781 tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg
    90841 ttatcaataa agatcgcttc gtcagtgaag cgattaccgc caaagttgaa gcggcgatta
    90901 aagaactcaa ttacgcgcca tcagccctgg cgcgtagcct caaactcaat caaacacata
    90961 ccattggcat gttgatcact gccagtacca atcctttcta ttcagaactg gtgcgtggcg
    91021 ttgaacgcag ctgcttcgaa cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg
    91081 aacagcggat gaatcgcaat ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt
    91141 tactgtgcac cgaaacgcat caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc
    91201 ctactgtgat gatggactgg gctccgttcg atggcgacag cgatcttatt caggataact
    91261 cgttgctggg cggagactta gcaacgcaat atctgatcga taaaggtcat acccgtatcg
    91321 cctgtattac cggtccgctg gataaaactc cggcgcgcct gcggttggaa ggttatcggg
    91381 cggcgatgaa acgtgcgggt ctcaacattc ctgatggcta tgaagtcact ggtgattttg
    91441 aatttaacgg cgggtttgac gccatgcgcc aactgttatc acatccgctg cgtcctcagg
    91501 ccgtctttac cggaaatgac gctatggctg ttggcgttta ccaggcgtta tatcaggcag
    91561 agttacaggt tccgcaggat atcgcggtga ttggctatga cgatatcgaa ctggcaagct
    91621 ttatgacgcc accattaacc actatccacc aaccgaaaga tgaactgggg gagctggcga
    91681 ttgatgtact catccatcgg ataacccagc cgacccttca gcaacaacga ttacaactta
    91741 ctccgattct gatggaacgc ggttcggctt agatttacgc tgtcttttga tcaaattatt
    91801 accatcggtt gttttcagaa gcatgaacat tgctgctgaa gcaacagtaa taatgcccat
    91861 cgtgataaac gtatagtgga attgttcgac agtcgttgtg ccttccatcc cttcataaac
    91921 gcgaaggacg gccgcactta cagcaacgcc taaactaatc gacagttgct gcgtgaccgc
    91981 cagaacactg ttaccgctgc tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt
    92041 catcgcggta aattgcgtcg acatagccat ccctaatata aacaacggca agatcagcat
    92101 ccatatcgcc attgccggtg attgcaaaga gaactgagcg atcattagcc caataatcac
    92161 cgtgatcccc actaacgtat ggcgatagcc cagacgacgt aagacttggg taaccatcga
    92221 ttttgcaata atggaaccta acgctgtcgg tgccatcata cagccggcaa taaacgcctg
    92281 ataaccaaat cctacctgta acatcaatgg cataaggaac ggtacacaac cggtccccag
    92341 acgggttgca atattgccta cgataccgat cgagaaagtg cgggttttaa ataaatctaa
    92401 tgaaattaat gggtttggcg tgtgtcgcgc atggagaata tagagaagca gtaacccgat
    92461 gctggtgaca attaccgtca aggcaatcca gctggcgaca atcctttccc cgaatagctc
    92521 tattccgctt gagaagagaa caaggctgag gccaaacagc aaaaagccag tgatatcgaa
    92581 tctgcgtcgt gcggtggtga aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat
    92641 acctatgggg atattgatta aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc
    92701 gccaagaacg gggcctaaaa ttggccccac cagacccggc atggcgacaa aattcaatac
    92761 tggaagaagt tcattacgag gataagcgcg cagtaaggcc agccgagcaa caggcatcat
    92821 cattgcgccg cctatcccct gaataacccg gaagacaacc agctgtggta gcgaattaga
    92881 aagtgcgcag gccagagaac ccaatgtgaa cagactcacg gcaagggtaa aaatgcgacg
    92941 cgtaccgaag cgatcggcta gccatccgct taccggaata agcatcgcca ccgtcagcgt
    93001 ataactgatg atggctgatt gcatcgcgag aggagaacga ttaaggctat gagcgattgc
    93061 gggtaaggcg gtattcagaa tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc
    93121 gatccacggc aaacccgcca tactgcgctt ctttttatcg ctcattcaat gtcctgttat
    93181 cgggttgtca cttatcaggt gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc
    93241 catcgctttg gacaatcgca tcgacaatcg cctggtgcag atccagcttt atcactgtgt
    93301 cgctggtaat tgacgtgaag taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg
    93361 tcaaaaacgg attggcgctc atttcataga tatgctcatg ccaggccata tcgacttcga
    93421 tccagcgttc acggcgaaag ttctctttta atgccgccat ttcggccatt aacgtattga
    93481 gatgcgcctt ctgttccgcg gtgccaaccg ttgcagccag taggcaagct tgcggctcca
    93541 gacagatacg cataaccaga aagtgatcga tgacctgatg aaagttctct tctgtcatcc
    93601 accaggtaag caattcctga tcaagaaaat tccagtttga ttgtggcatg acccgggtac
    93661 caattcgcgg tcgcggtaaa accatccctt ttgccgttaa cgttttgacc gcttcgcgta
    93721 ccgctgtacg actcactcca aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc
    93781 cgggttcata ttcacctttt aagatgcgtt gcgccagctt ctcagccaga acatacgaaa
    93841 ggtttttctg tgcagctaac tgttgtgcgc ttaaaggcat tactaatctt cctttttctt
    93901 tttattcctc cttagtatgc caccaggaag tgtgattacg gttgcaaaaa cggcaaattg
    93961 cttgttttat ggcacattaa cggggctttt gctgaaaaaa tgcgcggtca gaa
//
LOCUS       DAHBHC010000008        81521 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHBHC010000008 DAHBHC010000000
VERSION     DAHBHC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.008"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            122..460
                     /locus_tag="M2A35_000738"
     CDS             122..460
                     /locus_tag="M2A35_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197079.1"
                     /translation="MAKSAAERKAAQRARQAASGVRKLEIVLDAQEIEMLERNCATRR
                     PGRAPYEFGEYIALLIRQDDARVRGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            501..1175
                     /locus_tag="M2A35_000739"
     CDS             501..1175
                     /locus_tag="M2A35_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCB7197080.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEPKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLELIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            1172..2647
                     /locus_tag="M2A35_000740"
     CDS             1172..2647
                     /locus_tag="M2A35_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCB7197081.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRQWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKDWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNACKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRLPGQQNQQGKALTEYDPYA"
     gene            complement(2738..3109)
                     /locus_tag="M2A35_000741"
     CDS             complement(2738..3109)
                     /locus_tag="M2A35_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197082.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQPHESLSLLTFCVLVLRNGFTVTGESACASPENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(3308..3384)
                     /locus_tag="M2A35_000742"
     tRNA            complement(3308..3384)
                     /locus_tag="M2A35_000742"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3348..3350),aa:Arg,seq:tct)
     gene            3811..4017
                     /locus_tag="M2A35_000743"
     CDS             3811..4017
                     /locus_tag="M2A35_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197083.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            4032..5711
                     /locus_tag="M2A35_000744"
     CDS             4032..5711
                     /locus_tag="M2A35_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7197084.1"
                     /translation="MAETTKERLNKQFAQLESERQSFEPHWRELSDYINPRGSRFLTS
                     EVNRNDRRNTRIIDSTGTMAARTLASGMMSGITSPARPWFRLATPDPEMMDYGPVKLW
                     LEAVQNRMNDMFNKSNLYQSLPQLYGSLGTYSTGAMAVLEDDEDIIRTMPFPIGSYYL
                     ANSPRGSVDTCFRKFSMTVRQLVQEFGLNNVSESVKSMWESGTYEKWIEVMHSVYPNI
                     DRDTSKLDSKNKPFKSVYYEVGGDNDKLLRESGFDEFPIMAPRWEVNGEDVYGSSCPG
                     MLALGPVKALQLLQKRKSQLIDKATNPPMVAPTSLKNQRASLLPGDITYIDQITGQDG
                     FRPAYLVNPSTADLVADIQDTRQIINSAYFVDLFMMLQNINTRSMPVEAVIEMKEEKL
                     LMLGPVLERLNDECLNPLIDRSFSMMVRKNMLPPPPDAMEGMPLKVEYISVMAQAQKS
                     IGLSSLASTVNFIGQLAQAKPEALDKLNVDQAIDAFADMSGVSPTVIVPQEQVEQARQ
                     QRAQQQQQQQMMAMGMAAAQGAKTLSEAKTSDPSVLSAMANAVSGQGGQSQ"
     gene            5708..6004
                     /locus_tag="M2A35_000745"
     CDS             5708..6004
                     /locus_tag="M2A35_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197085.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            6007..6702
                     /locus_tag="M2A35_000746"
     CDS             6007..6702
                     /locus_tag="M2A35_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCB7197086.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPTGDNPAPVGDPS
                     QQEGDKPQPVADVDKPDDEKKSENDKQGEKKDGDKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            6717..7703
                     /locus_tag="M2A35_000747"
     CDS             6717..7703
                     /locus_tag="M2A35_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197087.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDANGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            7755..8192
                     /locus_tag="M2A35_000748"
     CDS             7755..8192
                     /locus_tag="M2A35_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197088.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGKRRDIGVGYPLE
                     FWALVNEAATASGEATVNIQLQTSEDNSSWTTIYDSGALAKTALTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGEFTAGISLDVDANTPYPIRSKVTG"
     gene            8203..8538
                     /locus_tag="M2A35_000749"
     CDS             8203..8538
                     /locus_tag="M2A35_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197089.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVTMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            8589..8912
                     /locus_tag="M2A35_000750"
     CDS             8589..8912
                     /locus_tag="M2A35_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197090.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPQGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            8912..9517
                     /locus_tag="M2A35_000751"
     CDS             8912..9517
                     /locus_tag="M2A35_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197091.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTDGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNRVILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            9517..11988
                     /locus_tag="M2A35_000752"
     CDS             9517..11988
                     /locus_tag="M2A35_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197092.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGVAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTASASIFGAEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTGTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGEDNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRAEYTISVSGGAYFTSSDVGAQLQFPYTGAVPDT
                     GYEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNVATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITAEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VKIRQLDPLPLSVLAVIPRLTVGGF"
     gene            11988..12452
                     /locus_tag="M2A35_000753"
     CDS             11988..12452
                     /locus_tag="M2A35_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197093.1"
                     /translation="MIDVQIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANGELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            12452..12997
                     /locus_tag="M2A35_000754"
     CDS             12452..12997
                     /locus_tag="M2A35_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197094.1"
                     /translation="MCDPVIAGGAMLAMSGIQAYTQYQQGKYASKVAEANADIATAQA
                     NDAINRGNAEAEQRRRETRQRLGTQAATMGATGADLSTGNALDIFGDTAQFGALDSLT
                     TVNNAQREAYGYQVQAANYKAEASSARKQGNVGAATTLLTAPLKAYGAYQMFGGTWSP
                     FTQSTPAPIGAAAGTRLPGGL"
     gene            12997..15510
                     /locus_tag="M2A35_000755"
     CDS             12997..15510
                     /locus_tag="M2A35_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197095.1"
                     /translation="MPVVPTVSGRQVESRGVQSAGLQTFSQQGIGDAFVRAGTEAIDV
                     LGQAKQRANIALAQEASLNLSQISSDLLNNPETGLLNLKGKNAIGKGHEYTQQFDAQV
                     EQLAMSLPDEQARNAFMQQAQQQRIQFTTQAGRHEIGQINAYEEGQFQATLLNNGKNA
                     AALYGDNAAYVLANKQTFQQIEEYGVAHGWSNEQIQAKKIEFKEKVADAALSQWSANN
                     ATAFIQSNGELSDTAAGARRAVADSDSSERARGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRQGIDTVSEIINRWAPPADKNDTMSYIKAVCEQLG
                     VSADEPLDASNPDTLKALCAAIIHHENGSQPYSDQQLTAGVSAALGLSTIPTNTKRYT
                     GNAAFDAASPEAQASFMRQADQLRRQQQAEYKTMIDSQVRDATAAYMRGVEFPNPPGE
                     ADFIAAYGVREGNLRYTEFRNTQIAGQYIGSFRNMPTSSITAYVEQLRPDTGETGEGY
                     AARAALYDNVVSAASQVIKQRQADPVQFSLAAGQAKPIDMSNKDNFGQSVALRAAQVS
                     DLAKSYGTPLTFFSKDEANQIGVFFRDAPVSQQAAYLDTIRQSTGGGQVYMSALQQIS
                     ANAPSAAVAGILMDKPGGILAEKNWFNPDVSVSPETAAQTILAGAAARKGTDDAKGIP
                     MPKDADLRLEFSDMVKDAFAGDAQGASMAYEIAKDYYAGVMAKKGVVSGEIDNDIWKQ
                     AVNVATGGVHDYNGMGNVLLPWGMSAEQFDKQVNQAWNEQVVGTGIKTPPGQYGLQSY
                     GDSQYLVKLGTGYLLKDDGSPVVLDLTQKRQRFSGDIPQ"
     gene            15507..17309
                     /locus_tag="M2A35_000756"
     CDS             15507..17309
                     /locus_tag="M2A35_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197096.1"
                     /translation="MSYFGLNPVNQNQQLDEAASNPVGFNSDVGFFDNAVGAALSGLY
                     SGLVAKPDQLLWAGMDKIVSPIAQLVNENTSLNDTSVSYIAEQRKLAEQQVKRLTPDA
                     ATTGTAGQVLYGLFDMGGQAVVGTTLGGPVGGAAAVTSLQGFSEFERLTAQGVDFRTA
                     QEAGLVQGITAGAGTLIPMSLGLRAGGALAEGVAAQLARTGESSVRRAAATAVRATPD
                     IAYAAGTNIAFGMAQRGLTAKTLRDGGYSEMANQYDVLDRQAIAIDAVLGVAFGGVGR
                     FINSRGESTSTPNFSPVDIDAALAANAAHHAEIDIAPGVPINVLSRNSHIQALRKAMS
                     DVSQGRPVDVASIVESASFSEIPGRKSLLSQAVNEALSSVDDGVTARAIENRLLEEQA
                     AQLLSRGDRQVYQSEIANSQRIIENLTEQRAQILAEEPTGSGKALSRARSDKQARLRD
                     IDQRIRQAQERLEFSRNALAPHEPGGQFFEARAELARRQQAESELNAQAVSFYKTAEV
                     RTPDEVAPFEPGKILQQTEQKMMADPAGDIDLRIAEDSLLESPDMIITVLDDDGNPQS
                     RSAREVLDEANRESEQAIQDSSLFDVAVACFLRG"
     gene            17315..19789
                     /locus_tag="M2A35_000757"
     CDS             17315..19789
                     /locus_tag="M2A35_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197097.1"
                     /translation="MRQECIQAVQQAAQRTLTAREIQSIEDRIYRNMRSIARDDPMSW
                     RQLSESERLYRAAQLASEELQREAALKKRRVALTIAARQRLDKFINSYQGADGKLGAL
                     NRTIAFNADGKSNFLSVESRTKATRDYALSQLQEAFEAVDPRFFGLFEDEAGVRDLVY
                     EMRGQNTGNAKARKGAKAWREVTDLLRRRFNDAGGDIGYLENWGFPQHHSMEKVGAVS
                     KDKWVSDVIGKLDRKYYIRADGQLMNDAELSAFLGEAYNTIATGGLNKLTDTGMRISG
                     ARANRGNASRQIHFKDADSYLQYQQLYGDRSLWEIMVGHLEGISKDIALVETYGPNPD
                     HVFRSLLDQVKAETATANPSKTGKVERLANNTENLYNFISGKTQPVANPHIARWSDNI
                     RNWLVASRLGSALLSSFSDLGTMYLSAKVTNLPMNQLFRNQLEAMDPTNRTELARARR
                     AGLAMESLLGSVNRWAMDNMGPSVSRWAATAVMRASGLTAWSDAHKRAYGVTMMGSLG
                     EVVSRTPDLRSLDDSDFRILKSKGITDTDWSVWKLAQQEDWGNGNNTMLTPESIMRIP
                     DSAVKHLGEPERVKFEAMRKLLGAVTEEVDMAVITPGAREQLITGSGIQRGTWKGELT
                     RSVFLFKSFPISVVMRHWSRAMGMPSAGGRAAYIATFIASTTILGALSQQLNDLASGR
                     NPREMTGEDAAKFWLGALLKGGGLGLYGDFLLSDHTRYGSGALASMLGPVAGLVDDVV
                     KIAQGIPLNAVEGKSEQTGGDLVKLGKGLMPGANLWYLKAALDHIIFNQMQEYFSPGY
                     LRKMEQRSKKEFNQTYWWRPQDVTPQ"
     gene            19799..19939
                     /locus_tag="M2A35_000758"
     CDS             19799..19939
                     /locus_tag="M2A35_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197098.1"
                     /translation="MIAFILVVFALVALGVMNRKCIIDDGEFAVAVILILSGVAGYIG
                     LS"
     gene            complement(20044..20484)
                     /locus_tag="M2A35_000759"
     CDS             complement(20044..20484)
                     /locus_tag="M2A35_000759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197099.1"
                     /translation="MKFNIRISNSFLNGESNTPFAVDGPFLTDDEIKIIQRFLEDVAN
                     GRALVGKNKPSWVDDNHDKIPGSDNYEQENYWHYHCGPTWYPNTFKNYTINLNFNPGG
                     MHSNECIHYAKNDNEIVVVGFSREHIPFLSSDGNDNPLFYDEEE"
     gene            complement(20505..20672)
                     /locus_tag="M2A35_000760"
     CDS             complement(20505..20672)
                     /locus_tag="M2A35_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197100.1"
                     /translation="MTALKNRTQHNEQAKQCWDVIGKVMLGRASKERDKDMVYQTGTS
                     FSDFKAAFRSK"
     gene            complement(20772..21458)
                     /locus_tag="M2A35_000761"
     CDS             complement(20772..21458)
                     /locus_tag="M2A35_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-repressor protein"
                     /protein_id="HCB7197101.1"
                     /translation="MTANVTPSVFHFESEATIRAIVIDGNPWFVAKDVIKALQLTNPT
                     MSIKSLDDDERAKFNLGRQGETNIISESGLYTLILRCRDAVTPGTIPYRFRKWVTGEV
                     LPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVMQAMKIENKTYSAPLKPGYRSL
                     IHSPSGVLGLTENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKCLRDIQTHA
                     EYINDKAGFF"
     gene            21858..22586
                     /locus_tag="M2A35_000762"
     CDS             21858..22586
                     /locus_tag="M2A35_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197102.1"
                     /translation="MEVFWIVVGVVAVIIYVINQNKTKISDRTVVNHNKTIKTEDGEI
                     TINRTQVIEHTSTQFQKTGGNAPNISAPPAYDSAVIQTYYKQQELAKERQLIQPKPFT
                     AELPPGVSTRPAYHGRFPGDDISSQSSKKAPQAVSEPARIPSVSPPKEESANRVSSGS
                     KQCLRCRINLPYEKFRKSSKNPDGLTKWCARCLDGPKNTRHMKWCPICNVRRKRTSFY
                     PNNQNADGLMAWCKTCWDEHKAKR"
     gene            complement(22609..22866)
                     /locus_tag="M2A35_000763"
     CDS             complement(22609..22866)
                     /locus_tag="M2A35_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197103.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRSLVEGSHLMKLLRELDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTR"
     gene            23062..23550
                     /locus_tag="M2A35_000764"
                     /pseudo
     CDS             23062..23550
                     /locus_tag="M2A35_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            23629..26010
                     /locus_tag="M2A35_000765"
                     /pseudo
     CDS             23629..26010
                     /locus_tag="M2A35_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26133..26537
                     /locus_tag="M2A35_000766"
     CDS             26133..26537
                     /locus_tag="M2A35_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197104.1"
                     /translation="MSAGLTSESLNQWLSMGSLAAVIAGVPPEVALGALAGAVIFVTS
                     AVEYPIRRRVLLSMLSFLCGLLFYKPTASILIGVASLIPTITQDSFEKGIVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELIPGRKDDGNA"
     gene            26524..26832
                     /locus_tag="M2A35_000767"
     CDS             26524..26832
                     /locus_tag="M2A35_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCB7197105.1"
                     /translation="MVTHEFFLLITNAVICTGIAIRVVTFRRNGSQHRRWGGWLAYFL
                     IVAAASIPVRVAYAIWLRTPMAVDLSEVIINAVMLAAVLKTRGNVVQIFKITRSKHGD
                     "
     gene            26822..27451
                     /locus_tag="M2A35_000768"
     CDS             26822..27451
                     /locus_tag="M2A35_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HCB7197106.1"
                     /translation="MEIKQFQRAAGISEALAERWFLHITSAMKEFGISKPEDQAMFIA
                     QVGHESGGFTRLQENFNYSVTGLANFVRAGRLTQGQANALGRRAGEAALPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLLENPELLAQDEYAA
                     RSAAWFFSSKGCMKYTGDIARVTLIINGGRNGIDDRRARYIIASKVLAV"
     gene            27448..27930
                     /locus_tag="M2A35_000769"
     CDS             27448..27930
                     /locus_tag="M2A35_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HCB7197107.1"
                     /translation="MIWAFVKAYWKHLIIVAMLAVLATSGVVAWNVHGSRQYDAGYAQ
                     AKEDRKTEDERVRQHYEQEKAINERESQQRIDQARNDALDAAARAGRLQQQLVAIREQ
                     LRHYNAIVGAGSSAADTGVLLADVLSKSLERNRQLAEYADRAAEAGRVCEKQYDTLTR
                     "
     gene            complement(28150..28689)
                     /locus_tag="M2A35_000770"
     CDS             complement(28150..28689)
                     /locus_tag="M2A35_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCB7197108.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEAARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(28705..29223)
                     /locus_tag="M2A35_000771"
     CDS             complement(28705..29223)
                     /locus_tag="M2A35_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCB7197109.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(29534..29725)
                     /locus_tag="M2A35_000772"
     CDS             complement(29534..29725)
                     /locus_tag="M2A35_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCB7197110.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            29743..29895
                     /locus_tag="M2A35_000773"
     CDS             29743..29895
                     /locus_tag="M2A35_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197111.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            30077..32320
                     /gene="pdeF"
                     /locus_tag="M2A35_000774"
     CDS             30077..32320
                     /gene="pdeF"
                     /locus_tag="M2A35_000774"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCB7197112.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(32359..33900)
                     /gene="ppx"
                     /locus_tag="M2A35_000775"
     CDS             complement(32359..33900)
                     /gene="ppx"
                     /locus_tag="M2A35_000775"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCB7197113.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(33905..35971)
                     /gene="ppk1"
                     /locus_tag="M2A35_000776"
     CDS             complement(33905..35971)
                     /gene="ppk1"
                     /locus_tag="M2A35_000776"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCB7197114.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(36142..36780)
                     /gene="purN"
                     /locus_tag="M2A35_000777"
     CDS             complement(36142..36780)
                     /gene="purN"
                     /locus_tag="M2A35_000777"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCB7197115.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(36780..37817)
                     /gene="purM"
                     /locus_tag="M2A35_000778"
     CDS             complement(36780..37817)
                     /gene="purM"
                     /locus_tag="M2A35_000778"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCB7197116.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            38142..38768
                     /gene="upp"
                     /locus_tag="M2A35_000779"
     CDS             38142..38768
                     /gene="upp"
                     /locus_tag="M2A35_000779"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCB7197117.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            38854..40143
                     /gene="uraA"
                     /locus_tag="M2A35_000780"
     CDS             38854..40143
                     /gene="uraA"
                     /locus_tag="M2A35_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCB7197118.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            40193..40939
                     /gene="hda"
                     /locus_tag="M2A35_000781"
     CDS             40193..40939
                     /gene="hda"
                     /locus_tag="M2A35_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCB7197119.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(41077..41436)
                     /gene="arsC"
                     /locus_tag="M2A35_000782"
     CDS             complement(41077..41436)
                     /gene="arsC"
                     /locus_tag="M2A35_000782"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCB7197120.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(41457..42920)
                     /gene="bepA"
                     /locus_tag="M2A35_000783"
     CDS             complement(41457..42920)
                     /gene="bepA"
                     /locus_tag="M2A35_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCB7197121.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            43133..44194
                     /locus_tag="M2A35_000784"
     CDS             43133..44194
                     /locus_tag="M2A35_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCB7197122.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(44232..45083)
                     /locus_tag="M2A35_000785"
     CDS             complement(44232..45083)
                     /locus_tag="M2A35_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCB7197123.1"
                     /translation="MHEKQTLFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGV
                     FMAIGFTFYLSVIADTPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSR
                     GVISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHT
                     FTESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIP
                     FAIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRA
                     IYLRQES"
     gene            complement(45101..47113)
                     /gene="hyfR"
                     /locus_tag="M2A35_000786"
     CDS             complement(45101..47113)
                     /gene="hyfR"
                     /locus_tag="M2A35_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCB7197124.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHFDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRSMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(47143..47556)
                     /gene="hyfJ"
                     /locus_tag="M2A35_000787"
     CDS             complement(47143..47556)
                     /gene="hyfJ"
                     /locus_tag="M2A35_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCB7197125.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(47549..48306)
                     /gene="hyfI"
                     /locus_tag="M2A35_000788"
                     /pseudo
     CDS             complement(47549..48306)
                     /gene="hyfI"
                     /locus_tag="M2A35_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(48303..48841)
                     /gene="hyfH"
                     /locus_tag="M2A35_000789"
                     /pseudo
     CDS             complement(48303..48841)
                     /gene="hyfH"
                     /locus_tag="M2A35_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
     gene            complement(48851..50518)
                     /gene="hyfG"
                     /locus_tag="M2A35_000790"
     CDS             complement(48851..50518)
                     /gene="hyfG"
                     /locus_tag="M2A35_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCB7197126.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(50508..52094)
                     /gene="hyfF"
                     /locus_tag="M2A35_000791"
     CDS             complement(50508..52094)
                     /gene="hyfF"
                     /locus_tag="M2A35_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCB7197127.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(52099..52749)
                     /gene="hyfE"
                     /locus_tag="M2A35_000792"
     CDS             complement(52099..52749)
                     /gene="hyfE"
                     /locus_tag="M2A35_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCB7197128.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(52761..53869)
                     /locus_tag="M2A35_000793"
                     /pseudo
     CDS             complement(52761..53869)
                     /locus_tag="M2A35_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(53881..53991)
                     /locus_tag="M2A35_000794"
     CDS             complement(53881..53991)
                     /locus_tag="M2A35_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCB7197129.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(54008..54955)
                     /gene="hyfC"
                     /locus_tag="M2A35_000795"
     CDS             complement(54008..54955)
                     /gene="hyfC"
                     /locus_tag="M2A35_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCB7197130.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(54966..56984)
                     /gene="hyfB"
                     /locus_tag="M2A35_000796"
     CDS             complement(54966..56984)
                     /gene="hyfB"
                     /locus_tag="M2A35_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCB7197131.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESCSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQSLRVVFSALFRLRQQFDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(56984..57601)
                     /gene="hyfA"
                     /locus_tag="M2A35_000797"
     CDS             complement(56984..57601)
                     /gene="hyfA"
                     /locus_tag="M2A35_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCB7197132.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(57854..58324)
                     /gene="bcp"
                     /locus_tag="M2A35_000798"
     CDS             complement(57854..58324)
                     /gene="bcp"
                     /locus_tag="M2A35_000798"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCB7197133.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(58324..58896)
                     /locus_tag="M2A35_000799"
     CDS             complement(58324..58896)
                     /locus_tag="M2A35_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCB7197134.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            59042..59920
                     /gene="dapA"
                     /locus_tag="M2A35_000800"
     CDS             59042..59920
                     /gene="dapA"
                     /locus_tag="M2A35_000800"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCB7197135.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            59937..60971
                     /gene="bamC"
                     /locus_tag="M2A35_000801"
     CDS             59937..60971
                     /gene="bamC"
                     /locus_tag="M2A35_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCB7197136.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            61184..61897
                     /gene="purC"
                     /locus_tag="M2A35_000802"
     CDS             61184..61897
                     /gene="purC"
                     /locus_tag="M2A35_000802"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCB7197137.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            62051..62278
                     /gene="vapB"
                     /locus_tag="M2A35_000803"
     CDS             62051..62278
                     /gene="vapB"
                     /locus_tag="M2A35_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCB7197138.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            62278..62676
                     /gene="vapC"
                     /locus_tag="M2A35_000804"
     CDS             62278..62676
                     /gene="vapC"
                     /locus_tag="M2A35_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCB7197139.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            62823..63686
                     /locus_tag="M2A35_000805"
     CDS             62823..63686
                     /locus_tag="M2A35_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCB7197140.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            63701..65716
                     /gene="tmcA"
                     /locus_tag="M2A35_000806"
     CDS             63701..65716
                     /gene="tmcA"
                     /locus_tag="M2A35_000806"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCB7197141.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTLLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            65790..66488
                     /gene="ypfH"
                     /locus_tag="M2A35_000807"
     CDS             65790..66488
                     /gene="ypfH"
                     /locus_tag="M2A35_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCB7197142.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(66598..66798)
                     /locus_tag="M2A35_000808"
     CDS             complement(66598..66798)
                     /locus_tag="M2A35_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCB7197143.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(66826..67953)
                     /gene="dapE"
                     /locus_tag="M2A35_000809"
     CDS             complement(66826..67953)
                     /gene="dapE"
                     /locus_tag="M2A35_000809"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCB7197144.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(67957..68313)
                     /locus_tag="M2A35_000810"
     CDS             complement(67957..68313)
                     /locus_tag="M2A35_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCB7197145.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            68694..68753
                     /gene="ypfM"
                     /locus_tag="M2A35_000811"
     CDS             68694..68753
                     /gene="ypfM"
                     /locus_tag="M2A35_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCB7197146.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(68852..71965)
                     /gene="acrD"
                     /locus_tag="M2A35_000812"
     CDS             complement(68852..71965)
                     /gene="acrD"
                     /locus_tag="M2A35_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCB7197147.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(72129..73829)
                     /gene="narQ"
                     /locus_tag="M2A35_000813"
     CDS             complement(72129..73829)
                     /gene="narQ"
                     /locus_tag="M2A35_000813"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCB7197148.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            74035..76014
                     /gene="aegA"
                     /locus_tag="M2A35_000814"
     CDS             74035..76014
                     /gene="aegA"
                     /locus_tag="M2A35_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCB7197149.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            76082..76657
                     /gene="nudK"
                     /locus_tag="M2A35_000815"
     CDS             76082..76657
                     /gene="nudK"
                     /locus_tag="M2A35_000815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCB7197150.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            76783..77826
                     /locus_tag="M2A35_000816"
     CDS             76783..77826
                     /locus_tag="M2A35_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCB7197151.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(77922..79925)
                     /gene="tkt"
                     /locus_tag="M2A35_000817"
     CDS             complement(77922..79925)
                     /gene="tkt"
                     /locus_tag="M2A35_000817"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCB7197152.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(79945..80895)
                     /gene="tal"
                     /locus_tag="M2A35_000818"
     CDS             complement(79945..80895)
                     /gene="tal"
                     /locus_tag="M2A35_000818"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCB7197153.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            81184..>81521
                     /locus_tag="M2A35_000819"
     CDS             81184..>81521
                     /locus_tag="M2A35_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCB7197154.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVD"
BASE COUNT        20343 a        20136 c        21317 g        19725 t
ORIGIN      
        1 aagcgcgacg attccagccg tagaattgat gttccgtatg acggatatga aatcaagaca
       61 atcacacatc cgcacttcgg cgataaaccg gtaaatgtgt tcgcgataaa ggtaaatatt
      121 gatggctaaa tcagcagcag agcgcaaagc cgctcagaga gcaagacaag ctgcatctgg
      181 tgtgcgtaag ctggaaattg tgcttgatgc tcaggaaatt gaaatgctgg agcgtaactg
      241 tgccacgcgt cgccccgggc gtgcgcctta cgaatttggt gagtatatag cgttactgat
      301 ccgccaggat gatgcacgcg tgcgcgggcg tataaaatcg atcagcagaa aacgttgcgg
      361 taagtgcggc gagagagttc cagttaattc atgcccttgt aatggtgact cgcaatgctg
      421 ggtgactaaa ggctggcatg aaacgaaatt aatagtgtga catgtcacga gtagattatg
      481 catgatgaat ttgactggtt ttgaatactg ccgccaacta tggcggcttt attttgcatg
      541 gtactattac cacaacggta actattacca cggtggttat gatgcctgct gaacctaaaa
      601 cctataaacg caaatcaacg caatttaagc cactaacagc aatgcaggag gcttattgcc
      661 agtcatacat caaaacgcct gaaaaccaga ctcaggcagc gattaacgca ggattctccc
      721 caaatacagc ggcagttaaa gccagtgtca tgatgcgcga tgaacgcatt caaaaacgga
      781 ttgccgagtt gatggaggag cgcaacaaac gaatgcgcgt cagtgctgat tacgttctca
      841 tgcgcctggt ggagatcgac cagatggacg tgattgatat cctcaacgac gatgggagcc
      901 ttaaaccaat ccgcgagtgg ccgaaaatat ggcgcactac gcttagcggc tttgatctgt
      961 catcgaccat catgaacatg aacgaggatt cgatagagac aatcctcaaa aaaattaaat
     1021 ggcctgacaa ggtgaagaac ctcgaactga ttggcaagca cgtcgatgtc aatgcattca
     1081 aagaacgcct ggatgttaat gtgaatgtga caattgctga tcgcatagcg gcagccagga
     1141 agcgactcaa agaacgtcag gatggtaatc agtgacagat acagcgttat ctcctgaaga
     1201 gcagttgatc gaggatattg cagggttcac tcacgatccg cttggctatg ccctctatgc
     1261 gttcccgtgg ggggaagagg ggactgaact ggcacatgcc accggcccac gtcagtggca
     1321 ggctgatgcg ttccgagaga tacgtgatca cctgcagaat ccagagacgc gctatcagcc
     1381 gcttatgctg gcacgcgctt ctggtcacgg tattggtaaa tccgcattca tctcaatgct
     1441 gatcaactgg ggcatgtcca cttgcgagga ttgtaaggtc gtggtgaccg ccaacaccga
     1501 caaccagcta cgaacgaaga cctggccgga aattatcaag tggtcgaacc ttgccatcac
     1561 gaaagactgg tttacctgta ccgctaccgc gatgtacagc aatgatcctg ggcacgacaa
     1621 gcggtggcga gctgacgcaa tcccctggtc tgagcacaac actgaggcat tcgccggact
     1681 acacaacgag cgcaaacgca tcatcgtggt tttcgacgaa gcatccaaca ttgccgatct
     1741 ggtgtgggag gtagcagagg gtgcgctgac ggacgaagac actgagatta tctgggtggc
     1801 gttcggaaac ccgacgcgta ataccggacg tttccgcgaa tgtttccgca aatataaaca
     1861 ccgctggaaa actgcgcaga ttgacagccg gacggtggaa ggcaccaaca aacagcagtt
     1921 gcagaaatgg gttgatgact acggggaaga cagcgacttc gttaaaatcc gtgtgcgcgg
     1981 catattccct gatgcatctg aattgcagtt tatccctacc ggtcttactg acgaggcaat
     2041 gaaacgggtg gtcaccgctg cgcaggttgc acatgctccg gtgataatcg gcgttgaccc
     2101 ggcatactca ggcgttgatg acgcggtgat atacctgcgg caggggctgc acagtaaggt
     2161 gctgtggacc ggcaacaaga ccactgacga tctgattatg gcaaagcgta tcgctgactt
     2221 tgaagaccag tatcaggctg acgcggtgtt catcgacttc ggctacggta ccggtctgaa
     2281 gtcaatcggt gacggctggg gtcgtacatg gcaacttgtt ccgttcggcg gcgcgtctac
     2341 tgacccgcag atgctcaata agcgcgggga gatgttcaat gcatgcaaga catggctgag
     2401 gctcggcggg atgctggatg accaggaaac agcggacgac ctgtcggcgg cagagtacaa
     2461 agttcgcgtg gacggtaaaa tcgttatcga accgaaggaa gatatcaagg agcggcttgg
     2521 gcgttcgccg ggtaaaggcg atgcgctact gctgacgttt gctttcccgg tgtcgaagcg
     2581 cctgcgactt cccgggcagc agaaccagca aggcaaggcg cttaccgagt acgatcccta
     2641 tgcttaatcc gttagcgggg ataatgacgg agatatcctc tggtgaggat aaaacaaatc
     2701 cagctcatcg gctggctgtt tgtgacatgt cacggtgtta ttgctcgctt aacttctgct
     2761 tcagtaagta accttcaagc atccagattt tgtttacagc attctgccgg gcaatcttcc
     2821 gaccaatttc cgcatcaaag ttttccgggc ttgcacaggc gctctctcct gtgacggtga
     2881 agccgttgcg cagcaccagg acgcagaacg tcagcagaga aagtgattcg tgcggctggt
     2941 agtttacctc tccgccagta tgtttcgctt ttatggcttt gccaaaggca ccatcttctg
     3001 ctgtgaaata tgcctcctga gcaataatgc cttcgatatg gtctggcgta acgcgcggtg
     3061 ccgttttgcc tttctcaacg atttcttttt cgatttgctg gtcgttcata atctcacctt
     3121 aaaaaaatgc ccggcgaacc gggcgaactg gaagcaatga gttatgcctt ccgtggctgt
     3181 actggtttac agcatgaagt catcgcaatg gcgtcctgct gtaaaaaggg cggtgatagt
     3241 tcttcaaggg aaaccatcac cgccaagcac ctggaacctc tggcatcacg gtccttaggc
     3301 gtgattctgg cgtggcatgc aggattcgaa cctgcgacca accgcttaga aggcgattgc
     3361 tctgtccaac tgagctaatg ccacaacgct gagagcactt agcctgttaa ggcgccacac
     3421 tttgtcgcgg ctccataaat gctctcatcg ttgtacctcg tctcttccga ggcgtcacac
     3481 cgaatcgccg ggatggtgaa tccccgtgcg cggaataaaa ccgctcgact tgcacattcc
     3541 ggctacctgg ttcgtttgcc cgagcaaggg agggtgcccc ttaaacgtac ccagaccgct
     3601 atcggcgcat gtgccatacg ccgtactact caaaataaaa gctcactcca cctgttcaat
     3661 ttaacgacaa gccagtcagg ttaataaccg gaatgaaccc tatatttacc ttgaaggtaa
     3721 taattcgtgc gttaaatgtc aaccacctac gataaataaa tcatatgtgg ttaaattggt
     3781 aataatttaa ttgcgtacgg agtcattgat atgtgcatgg gtagctcacc atcagtgcct
     3841 gcaacaccag aagttcaggc agcaccacag gagcaggatg ccgccgttgt tgatgcccgc
     3901 gacgaagaaa ctcgtcgccg tcgcgctgct gctggtcgta gttctacgct gcttaccggt
     3961 tctcagggcg acacatcaac cgctaatacc agcggtaaaa cgctgcttgg tcagtaaccg
     4021 gagtcattga aatggcggaa acaactaaag agcgattgaa caaacagttc gcacaacttg
     4081 aaagcgagcg tcagtcgttc gagccgcact ggcgcgagtt gagtgattac attaacccgc
     4141 gtggttcccg ctttctgact tctgaggtca accgtaacga tcgacgcaat acacgtatta
     4201 ttgattcgac cgggactatg gcggcgcgca ctctcgccag cggcatgatg tcaggcatca
     4261 caagccccgc ccgtccgtgg tttcgcctgg ctacgccaga tcctgaaatg atggattatg
     4321 gccctgttaa gttgtggctt gaggcggtgc agaaccgcat gaacgatatg ttcaataagt
     4381 cgaatctcta tcagtcgctg ccgcagttat acggaagcct cggcacatac agcactggtg
     4441 caatggcagt gctggaggat gacgaggaca tcattcgcac aatgccattc ccgataggca
     4501 gttactacct ggctaactca cctcgtggca gtgtggacac ctgttttcgc aagttctcta
     4561 tgactgttcg tcagcttgtt caggagttcg ggctaaataa cgtcagcgaa tccgtaaaaa
     4621 gcatgtggga aagcggcacc tacgagaagt ggattgaagt gatgcattcg gtttacccga
     4681 acattgaccg cgatacatcg aagctggata gcaagaacaa gccattcaaa tcggtttatt
     4741 acgaggttgg tggcgataac gacaagttgt tgcgtgagtc cggattcgat gagtttccaa
     4801 ttatggctcc gcgctgggaa gttaacggcg aagatgttta tggatcatca tgcccgggta
     4861 tgctggcgct tggacctgtt aaggcattgc agcttctcca gaagcgcaag tcgcagttga
     4921 ttgataaagc caccaatccg ccgatggttg ctccgacttc cctcaagaat cagcgcgcct
     4981 cccttcttcc tggcgacatc acgtatatcg atcagattac tggtcaggat ggtttcaggc
     5041 ctgcttatct ggttaacccc agtacagcag atttggtggc agacattcag gacactcgtc
     5101 aaatcattaa cagcgcctac tttgtcgatc tgttcatgat gttgcagaac atcaataccc
     5161 gctcgatgcc tgtggaagcg gtgatcgaaa tgaaagaaga aaaacttctg atgttggggc
     5221 cggttctgga gcgcctgaac gacgaatgtc ttaatcctct cattgatcgc tctttctcga
     5281 tgatggtgcg taaaaacatg ctgccgccac cgcctgacgc gatggaaggc atgcccctga
     5341 aggtcgaata catttccgtc atggctcagg cgcagaagtc tatcggcctg tccagtctgg
     5401 cgtccacggt taacttcatt ggtcaacttg cgcaagcgaa accagaagct ctcgacaaac
     5461 tcaacgttga tcaggcgatc gatgcattcg ctgatatgtc cggagtgtct ccaaccgtca
     5521 ttgttccgca ggaacaggtt gagcaggctc gccagcaacg ggcacagcaa caacagcagc
     5581 aacaaatgat ggcgatgggg atggcggcgg cacagggtgc caagacgcta agtgaagcta
     5641 aaacttcgga tccgagtgtt ttgtcagcta tggcgaatgc agttagtggt cagggtgggc
     5701 aatcacaatg acagattacg aagacgatca actgaaagaa gaaaacgccc gtaagcaacg
     5761 tgacatggcg cagcgtgaaa ttgatgacat tcgctttgtc atgagcagtg aacaggggcg
     5821 tcgcgttgtc tggtcggtgc tggagaaagg ccgggtgttt tccgctatct ctccgatgga
     5881 tgctatggca atggcattta atgaggggca acgcaatctg gcgctggaac tgtttcagcg
     5941 cgttatggcg cattgccctg aacagtattt gaagatggcc aaagaggcca gtgaacagga
     6001 gtgatcatga atttatttga gcgtttgctg tatcgccgtc tttgcaatga gcaaccagtc
     6061 gatggtggag cagctccggc tgcgtcagaa ccgtcagcgc ctacaggtga taaccctgct
     6121 ccagttggtg atccatcaca acaggaaggt gataagccac aacctgttgc tgatgtcgat
     6181 aaacctgatg atgaaaaaaa atctgaaaac gataagcagg gtgaaaaaaa agacggcgat
     6241 aaaccagagg gtgcgccgga gaagtacgaa tttcaggctg ccgaaggcgt agagctggat
     6301 acagaagcgt tgaaggaatt cgagccggtg gcgcgagaac ttaacctgac caacgagcaa
     6361 gcgcaaaagc tggttgatgc ttatccgaag attctggcag gtgttcagca gcgccaggca
     6421 gaagcctggc agaaaacaac cgagcagtgg gctgcggatg taaaagctga caaagaaatc
     6481 ggtggcgaca agttgatttc taaccttagc gccgcacagc gtgcgcttga ccagttcggg
     6541 acacctgaac tcaaagaata tctgaacacc accgggctgg gtaatcaccc tgatctggtc
     6601 aaaacgttcg tgaaaatcgg aaaggcgatg tctgaagatg gcatggtcac cggtggtaat
     6661 gaaggccagc gtagtgcggc cgaagtgctc tatggcaaat aagagaggaa atgacaatgg
     6721 ctgttaaagg cttaactgcg ctaacgctgg ctgactgggg taagcgcgtc gatccaaacg
     6781 ggaaagtcga taagattatc gagcttctcg gtcaaactaa cccgatcctt caggatatgc
     6841 cctttgtcga agggaacctt cctaccggac accgaaccac cattcgttct ggtttacctt
     6901 cagctacctg gcgtttgctg aactatggcg tacagccaag caaatcaacc acagtgcagg
     6961 taaccgattc cgttggcatg ctggaaacct atgctgaagt cgataagtca ctggctgatc
     7021 tgaacggcaa taccgccgaa ttccgcctgt ctgaagaccg cgcatttatt gaagcgatga
     7081 atcagcagat ggcgcagacg ctgttttatg gtgattccag cgttaaccct cagcagttta
     7141 tgggactgtc ctcccgctat tccagcctgt ctgcgggtaa tgctcagaac atcattgatg
     7201 ctggtggcac gggtacagat aacacctcaa tctggttagt ggtgtggggc gaaaacaccg
     7261 tgcatggcat cttcccgaaa gggcagaagg ctggcatcca gatggaagat aaaggccagg
     7321 tgacactgga agatgcgaat ggcggcaagt acgaaggcta ccgtacccat tacaaatggg
     7381 acaacgggct tgctctgcgt gactggcgtt atgttgttcg cattgcaaac atcgatgtca
     7441 gcaatctttc agaaccttcc tctgcagcaa atattgcgaa gttgatggtt aaagcactgc
     7501 atcgcattcc aaaccgtggc atgggccgcc cggtgttcta catgaaccgc actgtaggcc
     7561 aggctcttga tctgcaatct ctggagaaaa catctctggc gatcagcgta aaagagacag
     7621 aaggcgagtg gtggacttca ttccgtggtg taccaatccg tgagactgat gcgcttctgg
     7681 aaacagaagc ccgcgtggtg taacgcctgt tattaacctg tgggtcgtaa cagacccact
     7741 aatggagaaa gaagatgatc accgacaaac tgttgatgtt ctccgaagct caggcggtta
     7801 cgaataccgc ggcttctact gacgtaatcg atctcggtcc aattgacgga aaacgtcgtg
     7861 atatcggcgt gggttacccg cttgagtttt gggcgctggt taacgaagcc gccacggcaa
     7921 gtggtgaggc aactgtaaac atccagttgc agacgagtga ggataacagc tcatggacca
     7981 ctatttatga tagtggtgca ctggcaaaga ccgccctgac agcaggtaaa cgagttgttt
     8041 ctgcaaaggt gcctgccggt gttcagcgat atctgcgtgt taactactcc gtcgcaactg
     8101 gcccactaac ggctggcgaa ttcactgctg gtatcagtct tgatgttgat gccaatacgc
     8161 cgtatccgat ccgctcaaaa gtaactggtt aaggtgatat cgatgtcagg tgagaaacca
     8221 agataccgcg ttctgcgcct ctctcatatc cataacacac tgtggccgga gggggcagaa
     8281 atcgaatacg aaggtgagcc tggtagcgca ctggaacctg ttaacgatgc agccagacag
     8341 gcaaaagcaa aggtagcagg aaaggtgact atggcagcaa ccagcaccaa aatcatcaac
     8401 gatgtgtcag atgatggtga actggataag ctccgtgaag agtacgaatt gctctttaac
     8461 gagaagccac accataacgc caaagccgaa acgctccgcg agaagatcgc agataagcgt
     8521 aaagaactgg gcgtgtaagc ctcgcgaatc agacaagggg cttcggcccc tttattgcag
     8581 gagtgtatat ggaactcgta aacctcaaaa ccggcactga cagctaccag gatgagagcg
     8641 gagaaaccag aactcgcgat gaatacccgt gggggctgtg catcactctt aataacgaca
     8701 cattgaataa gctgaaggcg caacctcagg gcgtcggaac agaagtgatg ataactgcaa
     8761 aggctgttat tcgaggcctg tctgccagag aaactgacga tggtgttaat cgcagcgccg
     8821 atctgcagat cactgatatg gcgatcgctc ctgtttccgg ggatgtagaa aaatcagcgg
     8881 ctgaaactct gtacggtaac ggaggtgagt gatggcctct gtagtagaga tctgtaatcg
     8941 tgcgctgtcc aatattggca atagccgcag cattaacagc ctgacggaag ccagcaagga
     9001 agcgggggaa tgttcgctgc actttgaggc ctgccgtgat gctgtgcttt ctgattttga
     9061 ctggaacttt gctaccaaac gcgtggcgct tgcagatacg agcaatccac cgcctgactg
     9121 ggaatatgcg taccagtacc cgtcagattg tctgcgcatt actgaaatta tgcttcctgg
     9181 tgtacgcaat ccaacagcag caatgcgcgt tcagtacgaa gttggtgcag acaccgacgg
     9241 aacaggaaag ttgatctaca cagaccagcc gcaggcatgg ctcaagtatg tctctcgcgt
     9301 ttcagatgtg aacatgtttg atgccatttt tatggaggcg ttggcctggc gtcttgcggc
     9361 agctattaac atggcgctga ctgggaatgc agacctcggt acgtttgccc tcaatatgta
     9421 caatcgcgtg attcttagtg ctggctcgca tagccagaat gaatcacagg aaccacagcc
     9481 accggttgat gagtttacca ttgcgaggtt gtcctgatgg ctatcagttg gatccagccc
     9541 agctttgccg gtggtgagat tggaccgtcg ttgtacgggc gtattgacat ggcgaagtac
     9601 caggtagcat tgcgcaagtg cgataacttt atcgtgcggc agtatggcgg cgttgagaat
     9661 cgacctggta cgcgttttgt cggtgtcgcc aaatacccaa atcggaaatg ccgcctgatc
     9721 ccgttccagt tctcgacggt tcagacttat gctctggagt tcggacacca gtacatgcgc
     9781 gttatcaaag atggtgcgtt ggtgctgaac agcagcaatg ttatttatga aattgccacg
     9841 ccatatactg aagccgatct gttccgaatt aaattcacgc aaagcgccga cgtgcttacg
     9901 ctggttcacc cggcataccc gccgaaagag ctgcgccgct atgcgcatga caactggcaa
     9961 ctggttgatg tggtaacgaa gaacgggcca tttgaagata tcaatattga tgagtcagtg
    10021 acggtttatg ccagcgccag caccgggaca attacgttaa cggcaagcgc ctctattttt
    10081 ggcgcggagc aggtaggcaa attgttctat ctggaacagc ctgcagtgga ttctgtgccg
    10141 gtatgggaaa ccagtaagag tacgtcgatt ggcgatattc gccgtgcaga cagtaactac
    10201 tatcgcgccg ttacagcagg caaaacaggc actttgcgcc cttcgcatac agaaggcaca
    10261 tcatgggatg gctggggcgg atccggtgat gatgatactg gcattgagtg ggaatatctg
    10321 cacagtggtt ttggcattgc ccgtatcact gctgcaaatg gaactactgc aactgccgag
    10381 gtgatttcct atatcccttc gcaggtcgtt ggcgaggata atgccagcta taaatgggct
    10441 aaatatgcct ggaacagtgt taatggttat cctggcactg ttgtttatta tcaacaacgt
    10501 ctttacttcg ccgcatcgac tgcgttccct cagactatct gggccagccg tactggggat
    10561 tataaggatt ttggcaaaag caatcctacg caggatgacg acagaattat ctacacctat
    10621 gccgggcgtc aggttaatga gatccgccat ctgattgatg tcggttcgct ggtggcactg
    10681 acttccggag gtgagtacgt catcaccggc gaccagaaca aagtgttaac cccatcatca
    10741 tttgcattca gctctcaggg atcaaatggc tcaagcaacg tcccaccaat tgccgtggcg
    10801 aatattgctc tgttcgtcca ggagaaaggc agtgttgtcc gtgatctggc ctactcattc
    10861 gatgttgacg gctatcaggg gaacgacctg accatccttg ccaatcatct ttttcagaag
    10921 cacagcattg ttgactggtg cttctctatt gtcccttact ccagcgcctt ctgcattcgt
    10981 gatgacggta aattactggt gatgacctat ttgcgtgatc agcaggtttt tgcatgggca
    11041 ccacaatcca gtaccggaaa atatgaaagc acatgcagta tcagcgaagg aaatgaagat
    11101 gcggtgtatt tcgtcgttaa ccgaaccgtt aacgggcaaa cagtgagata catcgagcga
    11161 ctgtccagcc gtttatttac cagcgatgaa gatgctttct ttgttgattc tggccttagc
    11221 tatgatggaa gaaacacgtc tgacagaacg atgaccatca ctggtggttc tggcgaatgg
    11281 gattaccgcg cggaatatac aatcagtgtt tctggtggtg cgtacttcac cagtagtgat
    11341 gttggtgcgc aactacagtt cccttatacc ggagctgttc ctgatactgg ctatgaagtg
    11401 tcaaaagaat tacgttgcga cattatttct gtaaccagca ataccgcagt agtggttcgt
    11461 gctaacagga acgtcccgcc atccctcagg aatgtggcca ccacgaactg gcagatggcg
    11521 cgccggacat ttggcggctt gtctcatctt gaaggccaga ccgtaaacat cctctctgat
    11581 gcgaacgtgg aaccacaaaa agtggtttcc ggaggtgccg tcacgctgga atcaccgggg
    11641 gctgttgtgc acatcggcct gccaataact gctgaattcg aaacactgga tatcaacatt
    11701 aacggacagg aaacgctgct ggacaaaaaa caggtgatcc cgtccgttac tctggttgtg
    11761 aatgccagtc gcggcatctg ggcgactacg cccggcggta aatggtacga atatccacag
    11821 cgtgaattcg agttctacga tgatcctgtt gatgatgcta ccggaaaagt agaagtgaaa
    11881 ctggacagta actggggcaa aaacgggcgt gtaaaaatcc gtcagcttga cccgttgccg
    11941 ctgtctgttc ttgccgttat tcctcgcctt actgttgggg gattctgatg atcgatgttc
    12001 aaattattcc cgctaccgaa gagcatcttc agatgatttt gccggatgtt cgtcaggctg
    12061 atattgacga actgtatgcg gtatcgctga tgactaccga agatgcgctg cgcgttggtc
    12121 ttcgcactgc gactatggcc tggtcagggt tcgcgaacgg agaactggta accatgtttg
    12181 gtgtatctcc ggcgtcaatg atcggtggca atggtacgcc ctggctggta ggaaccagcc
    12241 gtattgaaaa atatcagaag acatttctgc gccactgccg ccctgtattg cagcagatgc
    12301 tggcagttta tccgcgcctg gaaaactacg tcgacgagcg aaaccatgtt gccaaagcat
    12361 ggctgcactg gcttggattc aggcttgaag aagccgcgcc ttatggtgct cttggtctta
    12421 atttccacag atttcacatg gagagaaaat aatgtgcgat ccggttattg ctggtggcgc
    12481 aatgctcgcc atgagtggca ttcaggcata cacccagtac caacagggaa agtatgcctc
    12541 gaaggttgca gaagcgaacg cagatatagc cacagctcag gcaaatgatg caataaacag
    12601 aggtaacgct gaagctgagc aacgacgcag agagacccga cagcggcttg gtacacaggc
    12661 ggcgacaatg ggggctaccg gcgctgattt atctacaggt aacgcgctgg atatatttgg
    12721 cgacactgcc cagtttggcg ctcttgattc gctgacgacg gtgaataacg cgcaacgcga
    12781 ggcttacggt tatcaggttc aggctgccaa ctataaagca gaagccagtt cagcccgtaa
    12841 acaggggaat gtgggagcag caacaacatt gctcactgcg cctctgaagg catacggtgc
    12901 ctaccagatg tttggtggga cgtggagtcc gtttactcaa agcactcctg cgccaatcgg
    12961 ggcagcagca ggaaccagat tacccggagg attataatgc cagtcgtacc aacagtatcc
    13021 ggacgtcagg ttgagagtcg tggagttcag tcagcaggct tgcagacgtt ttctcagcaa
    13081 ggtattggtg atgcttttgt tcgggcaggg acagaggcaa ttgatgtttt ggggcaggca
    13141 aaacagcgtg ccaatatcgc tctggctcag gaggcatctc ttaacctcag tcagataagc
    13201 agcgatctgc tgaataatcc tgaaacagga ttgcttaacc tgaaagggaa aaatgctatt
    13261 ggaaaaggcc atgagtatac gcagcagttt gatgctcagg tcgaacaact ggctatgtcg
    13321 ctgccggatg aacaggctcg taatgctttc atgcagcagg cgcagcagca gcgcattcag
    13381 ttcactacgc aggccgggcg acacgagata gggcaaatta atgcctacga agaaggccag
    13441 tttcaggcga cactgctgaa caatggtaaa aatgccgcag cattgtatgg cgacaacgcc
    13501 gcatacgtat tggctaacaa gcaaactttc cagcaaattg aggagtacgg tgttgcacat
    13561 ggctggagca acgagcaaat ccaggccaag aaaatcgagt ttaaagagaa ggttgctgat
    13621 gctgcattgt cccagtggtc ggcaaacaat gcgaccgcat tcatccaaag taatggcgag
    13681 ttaagtgata ctgctgctgg agctcgccgt gctgtagcag atagtgactc ttccgagcgt
    13741 gcccgtggca tacgcaacaa taacccagga aatctcgaat acagcaaaac taatccgtgg
    13801 gtaggccaga ccggtgatga tggtcgattt gctaaattcg aaacacctga acacgggatt
    13861 cgtgcattag ggcggaacct gatgtcgtat cagaggcagg gtattgatac cgtcagcgag
    13921 ataattaatc gctgggcacc gcctgctgat aaaaatgaca ctatgtcgta tatcaaagca
    13981 gtgtgcgaac aacttggcgt ttctgctgat gagcctctcg atgcatctaa tcctgatacc
    14041 ctgaaggcgc tttgtgcagc cattatccat catgagaacg gtagccagcc atacagtgat
    14101 cagcagttaa ctgctggtgt cagtgcagca cttggtttat caacaattcc aaccaacacc
    14161 aaacgctata ccggtaatgc agcattcgat gcggcatctc ctgaggcgca ggcaagtttt
    14221 atgcgacagg cggatcaact gcgtcggcag cagcaggctg aatataaaac gatgattgac
    14281 agccaggttc gcgatgcgac agctgcgtat atgcgtggcg ttgaatttcc taacccacct
    14341 ggtgaggctg attttattgc agcttatgga gtaagagaag gaaacctgcg atataccgag
    14401 ttcagaaata cgcaaatcgc cggacagtat ataggctctt tccgcaacat gccgacaagc
    14461 agcattacag catatgttga gcaattacgc ccggatactg gtgagacagg ggagggttat
    14521 gcggcacgag ccgctcttta tgacaacgtt gtgtcggctg caagtcaggt gataaagcag
    14581 cgacaggctg atcctgtaca gttctctctt gccgccggac aggcaaagcc tatcgacatg
    14641 agcaataagg ataactttgg acagagcgtt gccttgcgtg ctgctcaggt cagtgacctt
    14701 gctaagtcat atggcactcc actgacgttc ttttccaaag acgaggccaa tcagatcggt
    14761 gttttctttc gtgatgcgcc cgtttcccaa caggcagcat atctcgatac catcaggcag
    14821 agcactggtg gtgggcaggt gtatatgtca gcactacagc agatcagtgc caacgctcca
    14881 tctgctgccg ttgccgggat actgatggat aagccaggtg gtattttggc agaaaaaaac
    14941 tggtttaatc cggatgtttc cgtgtctcca gaaaccgctg cgcagacaat tcttgctggc
    15001 gcggcggctc gtaaaggtac tgatgacgcg aaaggtattc cgatgcctaa agatgctgat
    15061 cttcgccttg agttttctga catggtgaag gatgcatttg ctggtgatgc tcagggggca
    15121 tcaatggcat acgagatcgc aaaggactat tacgctggtg tgatggcgaa aaaaggcgtg
    15181 gtatcaggcg aaattgacaa tgatatctgg aaacaggctg ttaacgtagc tacaggtggc
    15241 gtgcatgact ataacggaat ggggaatgtt cttttgccgt ggggaatgtc tgcagagcaa
    15301 ttcgataagc aggttaatca ggcttggaat gaacaagttg ttggcacagg gataaaaaca
    15361 ccgcctggtc agtatggttt gcaaagttac ggcgatagtc agtacctggt gaaacttggt
    15421 actggttatc tgctaaaaga tgatggttct cccgttgttc ttgatctgac acagaagcgt
    15481 cagagattct ccggagatat tccgcaatga gttactttgg ccttaatcca gtaaaccaga
    15541 atcagcagct tgacgaagca gcatcaaatc cagttggctt taacagcgat gttggttttt
    15601 tcgacaatgc tgtaggagcg gcattgtctg gtttgtactc cgggctggtg gcaaagccag
    15661 atcagttgct atgggcaggg atggataaaa tcgtatcacc gattgctcag cttgttaacg
    15721 aaaacacctc gctcaatgac acttcagttt catacattgc tgagcagaga aaactagcag
    15781 agcagcaggt taagcggctg acgcctgatg ccgcgacaac cggaaccgcc gggcaggtcc
    15841 tttatgggtt gttcgatatg ggcgggcagg ctgttgtcgg tacaacgctc ggtggtccgg
    15901 tcggaggtgc tgcggcggta acttctctac agggtttttc tgagtttgaa cggttgacag
    15961 cacagggtgt tgatttcagg acggcgcagg aagcgggatt agtgcagggt attactgctg
    16021 gtgccggaac actgatccct atgagcctcg ggttacgtgc tggtggtgcg ctggcggaag
    16081 gtgtggcggc tcagcttgcg cggacgggtg aaagttcagt gcgacgcgcc gcagcaacag
    16141 cagtacgtgc aacgccagat attgcctatg ccgcaggtac aaatattgcg ttcggtatgg
    16201 cacagcgtgg gcttactgca aaaacgcttc gtgatggtgg ctatagcgaa atggctaacc
    16261 agtatgatgt gttggatcga caggcaattg ctattgatgc tgttcttggg gtggcgtttg
    16321 gtggtgtcgg cagatttatt aactctcgcg gcgagtctac aagcacacca aatttttcac
    16381 cagttgatat tgatgctgca ctggcggcga atgccgctca tcatgctgaa attgatattg
    16441 ctcccggcgt tccgatcaac gtgctttcgc gtaattcgca cattcaggct ctgcgaaaag
    16501 ccatgtctga tgttagccag gggagacctg tagacgttgc cagcattgtt gagtctgcat
    16561 ctttcagtga aattcctggg cgcaagagtc tgctgtctca ggcagttaat gaggctctgt
    16621 catctgtaga tgatggagta acggcgcgcg ctatagaaaa tcggttgctt gaagaacagg
    16681 ccgcgcagct tttgtcgcgt ggcgatagac aggtttacca gtctgaaatc gctaatagcc
    16741 aacgaattat tgaaaatctc actgaacagc gcgcacaaat tcttgcagaa gagccaaccg
    16801 gtagcggtaa ggctttatct cgtgctcgat cagataaaca ggccagactt cgcgatattg
    16861 accaacgaat ccggcaggca caagaacgcc tggaattttc tcgtaacgcg ttggcgccgc
    16921 acgagcctgg aggtcagttt tttgaagctc gagcagaact ggcacgacga cagcaggcag
    16981 aaagtgaact taatgctcag gctgtttcat tctataaaac agcagaggtc aggacgccag
    17041 acgaagtagc tccttttgag cccggtaaga tattgcaaca gacagaacaa aaaatgatgg
    17101 cagatccggc aggagatatt gatctgcgta tagctgaaga ctcgctgctt gaatctccgg
    17161 acatgataat caccgtgctg gatgatgatg gtaatccaca atcgcgcagt gcgcgtgaag
    17221 tactggatga agcgaacagg gaaagtgagc aggcaataca ggattccagc ctgtttgatg
    17281 tcgctgtggc gtgtttcttg agaggttaaa ttaaatgaga caggaatgta tacaagcggt
    17341 ccagcaggcg gcgcagcgca cgttaacggc gcgagaaata cagagcattg aagaccgcat
    17401 ttatcgaaat atgcgctcca ttgctcgtga tgacccgatg tcgtggagac aactttccga
    17461 atcagagcgg ctatatcgtg cagcacaatt ggcatctgaa gaattacagc gagaagcggc
    17521 attaaagaaa cgtcgtgtgg ccctcactat agccgcacgt cagagattgg ataaatttat
    17581 caatagctat caaggggctg atgggaaact tggcgctctt aaccgtacta ttgcttttaa
    17641 tgcagacggt aaatcgaatt tcctctctgt tgagtccaga acaaaagcca ctcgtgatta
    17701 tgcattgagt caattgcagg aggcattcga agcagttgat cctcgctttt ttggtctgtt
    17761 tgaagatgaa gcgggcgtac gtgacctggt atatgaaatg cgagggcaaa atactggcaa
    17821 tgctaaagca agaaaaggtg ctaaggcgtg gagagaagtg acagatctac tgcgccgccg
    17881 gtttaatgat gctggtgggg acattggcta tctcgaaaac tgggggttcc ctcaacatca
    17941 ttctatggaa aaggttgggg cggtatcaaa ggataagtgg gttagcgatg ttataggtaa
    18001 gctggatcgc aaatattata tccgagccga tggacaactg atgaacgatg ccgagttgtc
    18061 tgcatttctt ggagaggctt ataacacgat cgctactggt gggctgaata agcttactga
    18121 taccggaatg cgaatttccg gcgcacgtgc taaccgtggt aatgcatcac gacagataca
    18181 tttcaaagat gcagattcct atctgcaata tcagcaactt tatggcgatc gctctctatg
    18241 ggaaatcatg gtcggtcacc tggaaggtat cagtaaagat attgcactgg tggaaacata
    18301 tggcccaaac cccgatcatg ttttccgctc tcttcttgat caggtgaagg cagaaacggc
    18361 aacagctaac ccgagtaaaa ccggtaaagt cgagcggctg gcgaacaaca cagagaatct
    18421 gtacaacttt atttccggaa agacacagcc tgtagcgaat ccgcacatcg cgcgatggtc
    18481 tgacaatatc cgcaactggc tggttgccag cagactcgga tctgcgttgc tatcatcgtt
    18541 ctctgatctt ggaaccatgt atctgtctgc gaaggtgacc aaccttccaa tgaaccagtt
    18601 attccgcaac cagcttgaag ctatggaccc aacgaaccgt acagaacttg cgcgggcgcg
    18661 ccgcgctggt ctggcgatgg aatctctact tggcagcgtt aaccgctggg cgatggataa
    18721 tatggggccg tcagtgtctc gttgggcggc aacggcggta atgcgtgcca gtgggcttac
    18781 agcatggtca gatgcgcaca agcgcgccta tggcgtaacc atgatgggaa gcctgggaga
    18841 agtagtgtca cggacaccag accttcgtag cctcgatgac tctgattttc gtatcctgaa
    18901 aagcaaaggg attactgaca cagactggag cgtatggaag ctggcgcaac aggaggactg
    18961 ggggaacggt aataatacga tgctgacacc ggaaagcatt atgcgtatcc ctgattcagc
    19021 agttaaacat cttggtgagc ctgaacgcgt gaaatttgag gcaatgcgta aactgctcgg
    19081 tgccgtaact gaagaagttg atatggctgt tattacaccg ggagcacgtg agcaactgat
    19141 aaccggttct ggtattcagc gtggaacatg gaaaggtgaa ttaacgagaa gtgttttcct
    19201 gtttaaatcg ttccctatct cggttgttat gcgtcactgg tcacgcgcta tgggtatgcc
    19261 gtctgctggt gggcgtgcgg catatattgc gacgtttatt gccagtacga ccattcttgg
    19321 cgctttgtcg cagcaactta acgaccttgc gtctggtcgt aatcctcgcg agatgacagg
    19381 agaagatgct gctaaatttt ggcttggtgc tctactgaaa ggtggtggtc ttggccttta
    19441 cggtgacttt ttattgtcag atcacactag gtacggaagc ggcgcgctgg cgtcgatgct
    19501 tggcccggta gctggtctgg ttgatgacgt agtgaagatt gctcagggca taccgttaaa
    19561 tgctgtggaa gggaagagtg agcagactgg tggtgatctg gttaagctgg ggaaaggtct
    19621 gatgccaggt gcgaatctct ggtacttgaa ggcggctctc gatcacataa tctttaacca
    19681 gatgcaggag tatttttcac caggttattt gcgtaaaatg gagcaacgtt cgaagaaaga
    19741 gtttaaccag acatactggt ggcgaccaca ggatgtcact ccgcaataag gatgagaaat
    19801 gattgctttt attcttgttg tgtttgcgct tgttgcactt ggcgttatga accgtaaatg
    19861 tatcattgac gatggcgaat ttgctgttgc agttattttg atattatctg gtgtagcagg
    19921 gtacataggc ttgtcataac gtgagcgtga catgtcacag gccgctttcg cggccttgtt
    19981 tttaacgaat gccaccgccg cccggacggg aatccgcaga cacaaaaaag cccgcggcgc
    20041 gggctattct tcttcatcat aaaaaagcgg attgtcgttt ccatctgatg aaaggaaagg
    20101 tatgtgttct cttgaaaatc caacaacgac aatttcatta tcattttttg cataatgaat
    20161 gcattcatta gaatgcattc cgccggggtt gaaatttaag ttaatggtat aattcttaaa
    20221 tgtgtttgga taccatgttg gcccacaatg ataatgccaa taattctctt gctcataatt
    20281 gtctgagccg ggaattttat catggttatc atcaacccac gatggcttgt tttttccaac
    20341 aagcgccctt ccattggcaa catcttctaa aaacctttgt atgattttta tttcatcgtc
    20401 agtaaggaaa ggtccgtcta cagcgaaagg ggtgttgctt tccccattta gaaaactatt
    20461 agatattcta atgttaaact tcaagacaaa ctcctaatgt gcttctattt ggagcgaaat
    20521 gccgccttga agtcgctgaa agatgttcct gtctggtaaa ccatatcctt gtcgcgctct
    20581 ttgcttgcgc ggccaagcat aacttttcca atgacatccc agcattgctt ggcttgttca
    20641 ttgtgctgag tacgattttt caacgctgtc atatcgcgcc tcctttacct ttaaggtaat
    20701 agtacgctat tcacccccag tctgcaatct gtacagaatt atttaaaggc acatccctgt
    20761 gccgccgccc gtcagaagaa tcctgctttg tcgttgatgt actccgcgtg agtctggata
    20821 tcacgcaggc atttgctcac accgacgatg tagcagaaca tggtggtcag ctccgccgcc
    20881 gcgcccgata cgtcgtgccc gtcttcctgt aactggttca acagattcat cagcagtgag
    20941 ttctccgtca ggccgagaac accagacggc gagtgaatca ggctgcggta gccgggcttc
    21001 agtggggcac tgtaggtttt gttctctatc ttcatcgcct gcatcactgc tgatgctgtg
    21061 gcgttggcta cctggtcggc aaccatcttt atgcgttctt cctgcgggag cgagttttta
    21121 atgtaacttc cggtgcggcg gatctgagga agaacctcac ctgtaaccca tttacgaaag
    21181 cggtagggga tagtgcctgg tgtcaccgca tcgcggcagc ggaggatcag tgtgtagagg
    21241 cctgactcgc tgataatatt ggtttcgcct tgacgaccta agttaaattt agccctttca
    21301 tcatcatcaa gagatttgat tgacatggtg gggtttgtca gttgaagagc tttaataacg
    21361 tctttggcaa caaaccaagg atttccatca ataacaatgg ctcgaatggt tgcttctgat
    21421 tcaaaatgaa aaacagatgg ggttacgtta gcagtcatgg caatcacctt ttagtatggt
    21481 taatcaccac ctgaggtgca aatctcgttg gtggtgagac gtacagggtt tgcactaccg
    21541 gctaaaagga cccggcgcac ctttcggtgc ccctgcacgt cccaccataa ttctggcatg
    21601 actgtgctat acgcataaaa aaaccacgtc tggcgtggta tgcgcctttt agtaatccgg
    21661 ggtgcaaatc ccggcactgg attttgccag tgctcgatta ctatggcaca agaggagtgc
    21721 gatgtaaatt taccgcaaag gtaatgataa acgctgataa atataaaaat caaccgtatt
    21781 tggttgattg cgtttaacgc ttgatcacct gaaagcaaga tattaccttt aaggtaatgt
    21841 tattgtgagg aaaagcaatg gaagttttct ggatagttgt tggtgtggtt gcggtgatta
    21901 tttacgttat caaccagaac aagactaaga tctctgatcg tacggtcgtt aatcataaca
    21961 aaacgataaa gaccgaagat ggggagataa cgattaatcg tacacaggtg atagaacaca
    22021 cctctactca gtttcaaaaa actggaggta atgcgcctaa tatttccgca cctcctgctt
    22081 atgatagtgc ggtaatccag acatattata aacagcagga gttagcaaaa gagaggcaac
    22141 tgattcagcc aaagccgttt acagctgagc ttccacctgg agtgtcaacg cgtccggcat
    22201 atcatggaag attccctggt gatgacatat cgtctcagtc atctaaaaaa gcacctcagg
    22261 cagtatcaga gccagcaaga ataccttctg tatcgccgcc aaaagaagaa tcagctaaca
    22321 gagtttcaag tggtagcaag cagtgcttgc gatgcagaat aaaccttcca tatgaaaaat
    22381 tcaggaaatc gtcaaaaaat ccagatggat tgactaagtg gtgtgcaagg tgtctcgatg
    22441 gcccaaagaa tacacgccat atgaagtggt gcccaatttg taatgtccgc agaaaacgaa
    22501 ccagctttta ccctaataat caaaatgcgg acggcttaat ggcatggtgc aaaacgtgct
    22561 gggatgagca caaagcgaaa cgataggccg ctcttgcggc ctttaaattt accgggtttg
    22621 ttttcgtaat tgttcggcac aatagtcgag atgtgtttgc agatcctgca tagacatctg
    22681 tgagctggtg acgtagttaa tcagtgcagt cagttcggca agtgggccat cgacattaaa
    22741 tccatcctta tcgagctccc ggagtaattt catcaagtgc gatccctcca ccagtgatct
    22801 gacgcctccc ggcgtgtgaa tcctttcggt aaatccgtct tccagtggat agtgatactg
    22861 ctgcatctta tcttctccat gcaataactg tataaatata cagtatcaaa taatttattt
    22921 gctatccagc acgttttgcg aatcacctga aaggtaatat ctgttcgtat ttatgggttg
    22981 tctatccata tatggttttt caggtaatag aataaccgga tatgcggcgc aacgggtgct
    23041 gcgactatct ggagatttaa catgacggtc tcaaccgaag tcgaccataa cgaatacacc
    23101 gggaatggcg tcacgacgac attcccttat accttcagaa tttttcagaa gtctgatctg
    23161 gttgttcagg ttgtagacct ggatgaaaac ttgacagtat tggcacttga tacagattac
    23221 acggtaaccg gggccggtgg gtacactgga ggtaacgtca ttctgtcaac cgctctgaca
    23281 aatggatatc agatttccat atcgcgagaa attccggtta cgcaggaaac agatcttcgc
    23341 aatcagggta agttcttcgc agaggttcat gaggatgctt ttgataaact gacaatgttg
    23401 atccaacagt gttattcatg gacaaggttg gcgttgacaa aaccaactgt tatttctaat
    23461 ttttatgatg caaaacatca gtttattaaa aacttgcttg caccttttga tcgtaatgat
    23521 gctgcaaata aagggtatgt tgatgatatt tatcaatacc ttatgcagac agttaataca
    23581 attataaata ccatcaataa tggtctttat ggatataaca taagaagatc ttttgaatta
    23641 ggtaatacat taacatatcc aaacgatgtt ttattgtttg aaagtgatgg agaatattat
    23701 cgctgggatg gcccccttcc taagttagta cctcctgaat ctacaccgca aactacaggt
    23761 ggtattgctc caggtgcatg gcgatatgta agtgatgcaa cactacgaag cgccatatat
    23821 gaaactacaa aagtttataa atcaacaaat gatgcaatgt cttccatggt tgattcagtt
    23881 atcggttgta tcattactgg tggtagatac tcatttaatg atggtggtgg tgctacttac
    23941 atacgaacag ggatcggtac tgcaggtgac acagatggag gaagttactt tgttacccct
    24001 gatgggtcaa ggtttgactt agtatcacct gcaaatattt caacttttgg cggcgttcct
    24061 gggcatgact accgtgctgc tcttgacagt ctaaaatcgt atgtttcaac aagagataag
    24121 acaattagaa ttaattctaa aactattgtt agtatggtat ttacaccaga gattcctgct
    24181 ggatactgga atggattcat acttgacgta gatgatagcg tgacaattaa tactgcaagc
    24241 gatgaagctg tcaaatcacc attggtaaaa cttgtcagac ctgtaaaatt cttttttact
    24301 ggtaatagta ccagttattt attacgttca agtgttggaa ttacaaaagg cgaatataca
    24361 ccacttgttc ctggaacacc tccaacccct cccatcataa aaaatgcaaa ctacacagac
    24421 aataaaacgt tattttgcga tattccggat aaagaaacgc cagtattaac tgtctttact
    24481 tctgggtggt ctttcctgtc tggttcctgg cctatgggtg ttgttgcaga taccccagcg
    24541 gcgtcacttt ctggcgatgc tgtgtccatt ccagccccta cagggaatct tgtttttgct
    24601 gcatacagca gagtgaagcc gtgctctgaa tatttcgtgt cgatgtatga gggtgacaac
    24661 accggtggtg tgccatgcgt tgtagttgaa gctgaaaata tgcggtggat atattacgag
    24721 gatcgttttg gacatggtaa tttaattgag ggaacaattg gcggagcata ctctacaaaa
    24781 agcattgcat atgctggatc tgatactcat gcttcacaat ggctaaaaaa cagtattgca
    24841 tccgttcgtg ttgatacgct taagtcattc actatcctgg ttaacgggat agagcgagtt
    24901 acaattcgtc aaaccgggag aatcattggt cgtgtaggat ttggatcact tggcgatggc
    24961 ggtaagtata tgtctgtctt ctatccatac attatcgaaa atcgacaata ccgctctgca
    25021 aaaccgataa atatcgctgt tgtaggcgat tcaatcactg atgcctctat gggaaatgga
    25081 tgggtacagt tcgcgcaaca agcactagaa ggaactcatg gcattcgcgt tggacgtatg
    25141 cagacaattg caataagtgg gtatgcatca ggagatatcg ctaatatatt taatgccaca
    25201 tcgttggatg atatcgatgt agtgcttttc caaatcggag tgaatgatat tcaaaggcaa
    25261 tccggacgag aggcattacg agataacgtt aagcagatga tgctgactgc aaataacctt
    25321 gggattccct gcattgtatc tttgcctaca cagttctata ccagagatca gagtgttacg
    25381 ttaaccgggg ttgctggtta tggacagcct tcggcaaatt acgagcgtgg ggcgctttac
    25441 agggcgatac ttgccaacac agtagctgtt tgtaataaag aggttaatca gccgcttaat
    25501 gccctgaata ctggttctct tgacgatctc ggaccaatcg tagccgaata tctcctgaca
    25561 aatcaggacc ctgttgttat ggacaacatt cacccgacca catacggaag aattcttctc
    25621 ggcttgtcaa atgccagagc tattgcatca catatgttcg gtagacgtaa cgatcctata
    25681 attaaccact ggatgccagg tacgtggggg gcaaataact ggtcagtaac tcaactggtt
    25741 catgctgctg taacatcata tcccgatggt aaaattgcat taactggcac tatctctaac
    25801 accggaacag gtataaccgc agatggtacg acagtatgca accttccgtt gagcatctcg
    25861 ccagttagga aaatggcatt ttctgttaca aaacttgatg aaagtggcct ccctattgga
    25921 cagggtaata tcgttataga tactaacgga acaatcaaaa tatatggatt ctctacaggt
    25981 aacattagtc ttgatggcat aacatattga tataattaaa aatattatat gggtgtaata
    26041 aagtacgccc atatttaacc atatatggtt tattgtgtat gatggactca ccaactaagg
    26101 gggttcgtta tgcacagtaa acggtggtca ttatgtcagc cgggctaaca agtgagtcat
    26161 taaatcagtg gcttagtatg ggttcgcttg ctgctgtaat cgcaggtgtc ccgccagagg
    26221 tggcgttagg tgctcttgct ggtgcggtaa tttttgttac ctcagcagtt gaatatccga
    26281 tccgccgcag ggtgctcctg tcgatgctca gcttcctctg tggccttctc ttctacaagc
    26341 ccacagcatc cattcttatc ggcgtagcca gcctgatccc taccatcacg caggactctt
    26401 tcgagaaagg gatcgtcttc tctgctggcg cattcgtgtc agcaatcgtt gcagtgcgta
    26461 ttggcatctg gctctatcac cgttccgaca atccacgcga gttaatcccg gggagaaaag
    26521 acgatggtaa cgcatgagtt ttttttgctt atcaccaatg cagttatttg cactggcata
    26581 gcaattcgcg ttgtcacatt ccggcgtaac ggctctcaac accgaaggtg gggaggatgg
    26641 cttgcttatt tcctgattgt tgctgcggcc agtattcctg ttcgagtcgc ctatgcaatc
    26701 tggttacgta cgccaatggc tgtggattta tctgaggtca ttattaacgc tgtcatgctt
    26761 gctgcggttc ttaaaactcg cggtaacgtc gttcaaattt tcaaaataac gaggtctaaa
    26821 catggagatt aaacaattcc agcgtgctgc tggtattagc gaggcgctgg ccgaacgctg
    26881 gttcttgcat ataacttctg cgatgaaaga gtttggaatc agcaaacccg aagatcaggc
    26941 aatgtttatt gctcaggtcg ggcatgagtc tgggggcttc acccggttgc aggaaaattt
    27001 caactacagt gtcaccggac tggctaactt cgttcgggct gggcgtctca cccaggggca
    27061 ggctaatgca ctgggtcgcc gtgctggtga agcagcatta ccacttgagc gacagcgcgc
    27121 tatcgccaat ctggtgtaca gcaaacgcat ggggaacaat gcccctggtg atggctggaa
    27181 ctaccgtggg cgcggactta tccagattac cggcttgaat aactatcgtg actgcggaaa
    27241 cggtctgaaa gttgacctgc tggagaatcc tgaactgctg gcacaggacg aatacgcggc
    27301 ccgtagcgcg gcctggttct tctccagcaa aggctgcatg aagtataccg gcgatattgc
    27361 acgtgtaact ctgattatca atggtggccg gaacggcatt gatgaccgcc gcgcccgata
    27421 catcattgcc agtaaggtgc tggctgtatg atctgggcat tcgtaaaagc atactggaaa
    27481 catttgatta tcgtggcgat gcttgctgtt cttgccacat caggagttgt tgcctggaat
    27541 gtgcacggca gtcgtcagta cgacgccggg tatgctcagg cgaaagaaga ccgcaaaact
    27601 gaagatgaga gagttcgtca gcactacgaa caggagaaag cgatcaatga acgtgaatcg
    27661 cagcagagga tcgaccaggc gcgcaatgat gctcttgatg ctgccgctcg cgctggccgg
    27721 ttgcagcaac agctcgttgc catccgtgag cagctcaggc actataacgc cattgtcggc
    27781 gctgggtcgt cagccgcaga caccggagtt ttgcttgccg acgtgctcag caaatctctc
    27841 gagagaaaca gacaactggc agagtatgct gaccgggcag ccgaagccgg aagagtctgc
    27901 gaaaaacagt acgacaccct gaccagatag catggcattt ttcatggtac tgatttccgg
    27961 tgacggtata taaaacggta tgggaaaaat tcatctttgg aaaaatgtta tcactcaatt
    28021 ggttatggtg tccgtgaata attgagtggg aatgatttta atccctgcac tatgaatgaa
    28081 caaaaccctc tgttactaca gagggttttt tatcttcaag aatcataggc ttgaagttac
    28141 taacatcgat taattaaacc agctgtccga tttgttctct tctgctttgc ccacgctttt
    28201 catcagatcg cgaccgcctt cagtcatatt tctgttggcg tcagcttcag attgcaccac
    28261 atcggtttgc gcagctttgt gcttcagttc ctgatcgata aattcgtttt ctcgcttaac
    28321 gcgggctgct tcttttgcca gcgccagttt ttgtttctga atctctaagc tgcgtagctc
    28381 atcttcataa ctttgatcgc gttttttgtc cgcagaggct tcggcgtcca gtttatcctg
    28441 acgagctttc ttatttgctg ctgccgttgc cgctcttttg ttagcggcgg cctgggcatt
    28501 tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc tccgcgaccc attcgtcatg
    28561 ctgcttttgc tcttcatttt taccttgctg ttccgcttct gcgacagccg agagttgatc
    28621 ctgcaatgat gaggcgatag ccggatagct taaggaggcc aagatggcgc aaagaaaaac
    28681 tttcttcatg actcctcctg attattagct cttttcagga catttagtat ttggctgaat
    28741 acgcgtttcg ttatacgtcg tggtaataac aacggctaaa cctgtcgtaa actggcactc
    28801 tttacccacc tgggtggagg tatacacttt ggtgccttcc ttatacgtta aagaaacacc
    28861 ttccactaag gttttatcat tcaccataga acccgctgcc gcgcctacag ctccgccgcc
    28921 aactgcccct gccgtcgttc cggaattgct gccagaaccg acgttgtggc cgataacacc
    28981 gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg ttccgtttat tttgggagtt
    29041 gtctacggca acttttgcgg gaagaatgga aataatatta acggttttag tttcttgttt
    29101 ggtattcagt tgatcggttt gataaacatc ggcggcatga tcgtcagcat ttgactggca
    29161 tcctgccaga gtgaatgacg ctaacattgc tacaggcaga agacattttt taaatttcat
    29221 cactattcct tgttatacac attacgaaaa ataagacatt taagatcatc aaaagaggaa
    29281 agtaaatgta ttgcgtataa agggatagat taaatttatt aaaattcacg ccgggaataa
    29341 tttataaaaa tatatcatta caacatatat attaatttta tatttatttc gcagccaaac
    29401 aggataaaca tcagtcagga caaagacgta aaatttaaga tgaaagaata tctgccgctt
    29461 tgataatccc aaagagcaaa agcatccgac agcgaagtgc cgttaggttc tgtgaaagtg
    29521 gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga gagtgtggat tgctgagctt
    29581 cttttttgct ctgatggtgc tgccaggcac cgacggacga gtagataaaa cggccaaaga
    29641 agaagataaa gctgatgagc agtacgatac gggtcatgcg actgttaaat cggtgtcgtt
    29701 ttcgcatact ggttgcctga ctcacaaaag gttccttgaa gtatgtccca cgctgtggac
    29761 ggtacttaca ttaaggcaca acaggacaaa atggtcaatt cttcgttatg taaaaaagcg
    29821 tcagtggata catattttaa tgttatggaa gttaatttaa ttatttacaa tgatgatgta
    29881 ataatgatga attaacatga taaacgtaat aattcattaa tcagggttat tttgtttgat
    29941 atatatcaat tggaactttc atatgacact tagaatcaat gctctctata gtgatgaata
    30001 atcatcattc gaagtcaggt gggatgcctg tctgaataca cctccttcag gatgtggggg
    30061 attctgctga gcatctatga aactgaatgc aacttatata aaaatacgtg ataaatggtg
    30121 ggggcttccg ctgttcctgc cttctttaat cttgcccatt ttcgcccaca ttaatacttt
    30181 cgcgcatatt tcttccggtg aggtttttct cttttatctg cctttggcac tgatgatcag
    30241 catgatgatg tttttcagct gggcggcatt gccagggatc accttaggga tttttgtccg
    30301 caaatatgca gagctgggtt tttacgaaac gctctcatta acggctaatt ttattatcat
    30361 tatcattctc tgttggggcg gttacagggt ctttactccc cggcgtaaca acgtttcaca
    30421 tggtgatacc cgtttaattt cccagcgtat attctggcag atagtgtttc ctgcaacgct
    30481 gtttctgata cttttccagt ttgctgcatt tgtaggatta ctggcgagca gagaaaatct
    30541 ggtcggtgtc atgcctttta acctcgggac tttaatcaat tatcaggcct tactggtggg
    30601 taatctgatc ggtgtcccgc tgtgctactt catcattcgg gtagtgcgaa atccatttta
    30661 tttacgtagc tattattcgc aattaaaaca gcaggttgat gccaaagtca ccaaaaaaga
    30721 gttcgcgctc tggctactgg cattaggtgc tttattgttg ctgttatgca tgccgttaaa
    30781 tgaaaaaagc acaattttta gcaccaatta taccttgtca ttattgttgc ccctgatgat
    30841 gtggggagca atgcgctatg gttataagct gatttcgctg ctctgggcgg tcgtgttgat
    30901 gatcagcatc cacagctatc aaaattacat tcccatttat cctggctata ccacgcagct
    30961 gaccataacc tcctccagtt atctggtatt ctcttttatt gtcaattata tggctgtact
    31021 ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg cttgcgtatg tggacccggt
    31081 ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg cgtgatgccc cctggtctgc
    31141 gctttgttat ttacgcatcc ctggcatgga aatgctggtt aagaactatg gcatcatgct
    31201 gcggattcaa tacaagcaaa aactttctca ctggctgtca cccttgctgg aaccgggtga
    31261 agatgtttat cagctttcgg gtaacgatct cgcgctgcgg ctgaatacag aatcgcacca
    31321 ggagcgcatt accgcactgg atagccatct caagcaattt cgtttctttt gggatggcat
    31381 gccgatgcaa ccgcagattg gcgtcagtta ctgctatgtg cgctcgccag tgaatcatat
    31441 ctacctgctg ctgggagagc taaatacggt cgccgaactt tccatcgtga ccaacgcccc
    31501 ggaaaatatg cagcgtcgcg gggcaatgta tttgcaacgc gaattgaaag ataaagtcgc
    31561 gatgatgaat cgactacagc aggcgctgga acacaaccat tttttcctga tggcccagcc
    31621 gattaccggt atgcgtggtg atgtttacca tgaaattctt ctgcgcatga aaggtgagaa
    31681 tgatgaactg atcggccccg atagcttctt gccggtcgcg cacgaatttg gtttatcgtc
    31741 gagtatcgac atgtgggtca ttgagcatac gctgcaattt atggctgaaa acagagcgaa
    31801 gatgcccgct caccgttttg ctattaatct gtctccaacc tcggtatgtc aggctcgttt
    31861 tcctgttgaa gtcagtcagt tgctggctaa atatcagatt gaagcgtggc aacttatttt
    31921 tgaagtcacc gaaagtaatg ctctgaccaa tgttaagcag gcgcaaatca ccttgcagca
    31981 tcttcaggaa ttaggctgcc agattgcgat tgatgatttc ggcaccggct acgccagcta
    32041 tgcacggctt aaaaatgtga atgccgatct gcttaaaatt gacggcagtt ttatccgcaa
    32101 tattgtgtca aatagtctgg attatcagat agtggcatcg atttgccacc tggcgcgaat
    32161 gaagaaaatg cgggtagtgg cagagtacgt tgaaaacgaa gagatccgcg aggcggtgct
    32221 ctctttgggg atcgattata tgcagggtta tcttattggt aagccgcagc cgttaattga
    32281 tacgttgaat gaaatcgaac ccattcgcga aagtgcctga ataatgcggg ccgacatttc
    32341 tcgtcggccc gcaaagcatt aagcggcgat ttcaggtgta ctttcttctt caattttcaa
    32401 ccgccagcca gccacgcctt cccagtattc ttgctccttt tccagatcaa gcagtaccag
    32461 cgcattctga ctaaaccagt catgcgggaa acgcagtgtc cagtgactgt catcagtaat
    32521 cagtgtcaat gttggcggtg tggtggttgc ctgacgttga ttgtttagta atacgccaag
    32581 gcgcaatagc tgtatcagtg gcaggaactg tttcttctta aacaaggtaa agcgcggcag
    32641 atcgtcgagc ttaatcgctt tacggtgata gcgcaccagt gtcgccatca tcagctgctg
    32701 ttcctgatta aaacccggca agtcactgtt ttgcagaata taagcggagt ggcgatgcaa
    32761 accgctgtgg ttgatattca acccgacctc atgcagcatg gcggcccatc gcagtagcgc
    32821 ctccagttgc ggatgcgcca gcttcggttg ctgttcccgc cactgttcgt acatttgcat
    32881 agtggtatcc agcactcgtc gggcctgttc gctgtcgatg tgatactggt tggcgaggct
    32941 gctggcggtg cgactacgca catcctgatg acggaaacgt ccttccattt catacagtac
    33001 gccttcgcgt aacgccccgt cagaaaggcg cagttcacgg atggctaaag catcaaacac
    33061 accgcataaa atcgccagtc caggaacgaa gactgttttc cgctcttcgg aaagacccgg
    33121 taaactcagc gatgcgaaat tacggtgacg taaaacttct tttaccagtt tttccagacg
    33181 ttccggggta attatcccgt ctttctcgcc catttccatt aacacttcat gggcggcttt
    33241 tatggtaccg gaagcgccca ttgccacgtt ccagccctga atacggaatt gccaggttaa
    33301 agtttccagt ttttgtgctg ccgccatacg agcgcgctga aaattctctt tattgatgac
    33361 cccgccaggg aagtaaagct gggcaaagct gacacaaccc atccggcggc tttcaacgag
    33421 aatcggttcg aaattttcac caatcaccag ttccgtagat ccgccgccaa tatcaataac
    33481 cagtttgcga cctttctccg gttgggtatg ttccacgccc ataaaaatca gacgggcttc
    33541 ttcattaccg gaaataattt caatcgggta gggaatgacc ttttccgcgc gtttcagaaa
    33601 gtcagtggcg ttcagcgcct gacgcagcgt atgggtgccg actatacaga cgctggcagg
    33661 agaaaaccct tgtagccgtt cggcaaacag cgacagacag ttcaaaccgc gcgtcattgc
    33721 ctcttcactc aacatattat ctggccccag gccgtccgcc agatgcaccc gctgtttcag
    33781 gcggccaata atctgcatgg caccatctac cacacgggct atgaccatgt gaaaactgtt
    33841 tgaaccaaga tcgaccgcag caaactcctg cggacgaggg gatttatcgt gtattggcat
    33901 agggttattc aggttgttcg agtgatttga tgtagtcgta aatcgccaac tgcgcccgta
    33961 ctttgcggcg attgccgcgg ggaacgtagc gattactgag ttctttatcg atataacgtg
    34021 ctttgaccgt atcgctgaac aatatgtcga tgatgtccag cacccgctgc ttcaggcgcg
    34081 gatcgagcag cggtgtcgcc acttcaatac gataatcaat attgcgcgtc atccagtcgg
    34141 cggaagaaag gtagaccttt ttatcgccgc cattttcaaa aatataaacc cggtcatgtt
    34201 caaggtaacg gtcaacaata ctgatggcac gaatgttgtc gctaatgcct tccagattgg
    34261 ggatcagcga acacattccg cgaaccagca gattaaccgg tacgccggag ctggaggccg
    34321 catacagacg atcaaccagg cctttatcga caaggttatt tagcttcagg gtgataccac
    34381 tgggcagccc ttgctgcgcg ttggcgattt cgcggtctac catttcatat aacagacggc
    34441 gagagttttg cggtgacacc attaaataat caaatgtcac cgggcggtat gggttttcaa
    34501 taaagttaaa tacccgccgt acttcgttgg tgatacgcgc atctgcggtc agcaacgaat
    34561 agtcagtata aagacgcgcg gttttttcgt taaagttccc ggtcccgatg tgtgcgtaac
    34621 ggaccacttc accgttttct ttacgtgaaa tcaggaacag tttggcgtga attttcagcc
    34681 ccggcgcaga gaagataacg tgcacgcctg cttcggtcag gcgcttcgcc cagtgaatgt
    34741 tggcttcttc gtcgaaacgc gcctgtaact caaccaccac ggtgactttc ttaccgttat
    34801 gtgcggcgtg gatcatcgag tcgatgatgc gtgaatcttt cgccacgcgg taaatgttaa
    34861 ttttaatcgc cagtacgctc gggtcgaacg aagcctgacg cagcagttcc agcacatgct
    34921 caaaggtgtg ataaggataa tagagcaaca catcgcgttc gcgaatggca tcaaaaccat
    34981 tgcggaactg ggctttatca aaccaaatgt ggcgtaaacg cggcagtggt ttgttcacca
    35041 gattggcttt gccgacattg gggaaattaa taaagtcttt aaaattatga taacgaccgc
    35101 cggggacgat ggagtcgtag cgggaaatag tcagtttttc gcgtaacact tcaaccagcg
    35161 cattgggcat atcgcgctga taaacaaaac gcaccggctc agcagttaaa cgctgcttga
    35221 gactggaaga cattaactcc atcaggctgg cttccatctc atgcactaaa tcgtattcgg
    35281 catcgcgggt catcttcatt gaataggcat tcagcgcgtc ataatcaaag aagcctttga
    35341 aaatatcatc gaggcagtaa cgcagaatgt tatccaaaag aatcatcggc ttgcgtcgac
    35401 gcggtgcttc tggcggtaaa ttcacaaagc gcggcacttt atctgatggg atctccagca
    35461 gcgcgtaacg gatggtatcg ccacggataa tttccaccgc cagataggtg taatcatctt
    35521 tcaggaactg cactaagtca gtgtcaggat tgattaaaat cggcgtaatg tgctgacgca
    35581 gatactgctt aaaataatga cgcagccagt tttgttgatt gacggagagc tggcgttcat
    35641 taatcaggaa gatctggttg cgcgccatct ccagcaatag ctcgttgtag aggccgtcga
    35701 attcctgatc ggctttcagc acccgggact gaattttgcc cagtaaatgg cgggaatgag
    35761 agttggagcc ttgttcttcg ctaataatga tgcgtcgctt cagttcagcg aagcggactt
    35821 tatagaactc atcaaggtta ttggaataga tccccaggaa acgcatcctt tcaatcagcg
    35881 ggttagattt gtccgccgct tcctgaagca cgcgttcatt gaacgataac caactgagct
    35941 ctttttcgat gtatagcttt tcctgaccca ttaccacttt tactccgttt tattcacggg
    36001 acactgcctg gatattatgg cgagcaatat tggcagatgt ccaactatgc cagaaaaggg
    36061 ggaaaaaaac tgctggagct tgcgatttac aggtgaattg aaaaacgcca gcggcagagc
    36121 tggcgcttta actacggggc attactcgtc ggcagcgtag ccctgcggcg gcagacgttg
    36181 accatccagc cacgcggcgt tttcgtgcat tttcagacga ccatcggcaa accagctaat
    36241 caccagtgga taaatagcgt gttcctgggt ttgcacgcgg gcggtgatgt catcttccgt
    36301 atcaccagca aataccggga ctttcgcctg taaaataacc gggccaccgt ccagttcatc
    36361 ggtgacgaaa tgcaccgatg taccgtgctc ttcatcacca ttttccagcg cctgacgatg
    36421 ggtgtgtaat ccgggatatt tcggcagcag agaagggtga atgttcagca aacgcccggc
    36481 atagtgggag acaaacgccg ggctgagaat gcgcataaaa ccagccagca cgaccacatc
    36541 gggtgcgtac atgtcgattt catgaatcaa ctcccggtca taggcttcac gactgtcaaa
    36601 cgcgctggcg atgagcgtat gcgttgcaat acccgcctgg cgggcgcgtt caaggccgaa
    36661 cgcgtcggcc ttattgctga aaactgcccg tacggtgcct ttaattttgt tagttttaca
    36721 ggcgtcaata attgcctgta aattacttcc gttgccggaa ataagcacca caatattcat
    36781 tattcgataa ccacgcgttg ttcggaatca gaggctttga tgataccgat tttccacgcg
    36841 ttttcaccgt tggagtttag cagggcgagg gctttgtcca cttccggagc aggcagggcg
    36901 ataatcatcc cgacgccgca gttgaaggtg cgatacattt catggcgctc aacgttacct
    36961 gccgtttgca gccagttgaa cacttccggc cactgccagg aagattcatc aatcactgcc
    37021 tgagtattat ctggcaatac gcgtggaatg ttttcccaga agccgccgcc ggtcaggtgc
    37081 gcaatggcat gcacatcgac cttttcaatc aactccagca ctgacttcac gtaaatgcgg
    37141 gtcggtgcca gcagatgatc ggctaatggc ttaccatcaa gttcggtggt ttgcggatca
    37201 caaccgctga cttcaagaat tttgcgcacc agcgaatagc cgttcgagtg cggaccgctg
    37261 gaaccgagtg caatcagcac atcgccgtcg ctgactttag agccgtcgat gatttctgat
    37321 ttttctacca cgccaacgca gaaacccgcg acatcgtaat cctcaccgtg atacatcccc
    37381 ggcatttctg ccgtttcgcc acccaccagt gaacagcccg attgcagaca tccttccgcg
    37441 ataccgctga tcaccgctga agcggtatca acatccagtt ttccggttgc gtaatagtcg
    37501 aggaaaaaca gcggctctgc accttgcacc accaggtcat taacgcacat ggcgaccaga
    37561 tcaataccaa tggtgtcgtg acgttttaag tccatcgcca gacgcagctt ggtacctacg
    37621 ccgtcagtac cggaaaccag cacaggttca cgatattttt gcggcaatgc acacagcgca
    37681 ccgaagccgc caagaccgcc catcacttcc ggacgacgcg ttttcttcac tacgcctttg
    37741 attcttccaa ccagagcatt acccgcgtca atatcaacac cggcatcttt gtagctaaga
    37801 gaggttttat cggtcactgc ttgggtcccc acgcgttact tgcggtagaa aaataaaatt
    37861 cggcgcaatt ctaacaggga aagcaaacgt ttgcgagact gctttacaca acctttttgc
    37921 acgtcttttg cccaggcacg cggcgaaaga agacttgtgc cagggtaaag gttagttttc
    37981 ggatggaata atcttctttc ataaccattt gaatataaaa taactttatc tcaaaccgtt
    38041 atcattttga ctaaagtcaa cgaaaagaat attgccgcct tgaagaaagg aggtataatc
    38101 cgtcgatttt ttttgtggct gcccctcaaa ggagaaagag tatgaagatc gtggaagtca
    38161 aacacccact cgtcaaacac aagctgggac tgatgcgtga gcaagatatc agcaccaagc
    38221 gctttcgcga actcgcttcc gaagtgggta gcctgctgac ttacgaagcg accgccgacc
    38281 tcgaaacgga aaaagtaact atcgaaggct ggaacggccc ggtagaaatc gaccagatca
    38341 aaggtaagaa aattaccgtt gtgccaattc tgcgtgcggg tcttggtatg atggacggtg
    38401 tgctggaaaa cgttccgagc gcgcgcatca gcgttgtcgg tatgtaccgt aatgaagaaa
    38461 cgctggagcc ggtaccgtac ttccagaaac tggtttctaa catcgatgag cgtatggcgc
    38521 tgatcgttga cccaatgctg gcaaccggtg gttccgttat cgcgaccatc gacctgctga
    38581 aaaaagcggg ctgcagcagc atcaaagttc tggtgctggt cgctgcgcca gaaggtatcg
    38641 ctgcgctgga aaaagcgcac ccggacgtcg aactgtatac cgcatcgatt gatcagggac
    38701 tgaacgagca cggatacatt attccgggcc tcggcgatgc cggtgacaaa atctttggta
    38761 cgaaataaag aataaaaata attaaagccg actttaagag tcggcttttt tttgagtaaa
    38821 gcgcctataa cacataatac agaggataat actatgacgc gccgtgctat cggggtgagt
    38881 gaaagaccgc cacttttaca gacaatcccg cttagtttgc aacatttgtt cgccatgttt
    38941 ggtgcaaccg tcctggtgcc cgtcttattt catattaacc cggcgactgt actgttattt
    39001 aacggtattg gaacgctgct gtatctcttc atctgtaaag ggaaaattcc ggcttatctt
    39061 ggttccagct ttgcctttat ttcaccggta ttgttactgt tgccgttagg gtatgaagtc
    39121 gcgctgggcg gctttattat gtgcggcgtg ctgttctgcc tggtttcttt tatcgtgaag
    39181 aaagcgggga ccggctggct ggacgtgctg tttccacctg cggcaatggg cgcaatcgtt
    39241 gccgtcatcg gtctggagct ggcgggcgta gctgccggta tggcgggttt actcccggct
    39301 gaagggcaaa cgccagactc caaaaccatc atcatctcta ttaccaccct ggcggtcacg
    39361 gttttaggtt ccgtgctgtt tcgtggtttc ctggcaatta tcccgatttt aattggcgtg
    39421 ctggtgggct acgcgctctc tttcgcaatg ggaattgtcg ataccacgcc gattattaat
    39481 gctcactggt ttgcgctgcc aaccctctat acgccgcgct tcgagtggtt tgccattctg
    39541 actattctgc cagcggcgtt agtggttatt gccgaacacg tagggcacct ggtagtaacg
    39601 gctaatatcg tcaaaaaaga tctgctgcgc gatccaggtc tgcaccgttc gatgtttgct
    39661 aacggcctgt cgaccgtgat ctccggcttc tttggctcta cgccaaatac cacttacgga
    39721 gaaaacattg gcgtgatggc gatcacccgt gtttacagta cctgggttat cggtggggcg
    39781 gcgattttcg ctatcctgct ttcctgcgtc ggtaaactgg ctgccgctat ccagatgatc
    39841 ccattgccgg tgatgggcgg cgtttcgctg ctgctttatg gtgtcatcgg tgcttccggt
    39901 attcgtgttt tgatcgaatc gaaagtggat tacaacaaag cacagaacct gatcctgact
    39961 tccgtgattt tgatcatcgg cgtcagtggc gcgaaggtaa acatcggcgc ggcggagtta
    40021 aaaggtatgg cgctggcgac catcgtcggt atcggcttaa gtttgatttt caaactgatc
    40081 agtgtgctgc gccctgaaga agtggtgctg gacgcagaag acgcggatat aacagacaaa
    40141 taatgtaccg accgggcagt gttgctgccc ggttcaaaca tcatgggatt ctgtggtaaa
    40201 cttctcgcga ttttgtgaaa tcctggttga ggtatctctg aacacaccgg cacagctctc
    40261 tttgccactt tatcttcctg acgacgaaac ctttgcaagt ttctggccgg gggataactc
    40321 ctctttactg gccgcgctgc aaaacgtgct gcgtcaggaa catagcggtt acatctatct
    40381 ctgggcacgc gaaggcgcgg ggcgcagcca tctgctgcac gcggcttgcg cggaattgtc
    40441 gcagcgtggc gatgcggtgg gctatgtccc gctggataaa cgcacctggt ttgttccgga
    40501 agtgctcgac ggtatggagc atttgtcgct ggtctgtatc gacaacattg agtgtattgc
    40561 aggcgatgag ttgtgggaga tggcgatttt cgatctctac aatcgaattc tggaatcggg
    40621 caaaacacgg ttgttgatca ccggcgatcg tccaccgcgg cagttgaatc tgggattacc
    40681 ggatctcgcg tcgcgactcg actgggggca gatctacaaa ttgcagccac tttctgatga
    40741 agataagttg caggcgctac agttacgcgc gcgtttgcgt ggttttgaac tgccggaaga
    40801 tgtggggcgt ttcttgctga agcggctcga cagagaaatg cgcacgctat ttatgacgtt
    40861 ggatcagttg gatcgtgcgt cgattaccgc gcaacgtaag ctgaccattc cgtttgtgaa
    40921 agaaattctg aagttgtaga taaggtgttt attgtcggat gcggcgcgaa cgccttatcc
    40981 gaactacgcg aagttaatcc atagtattcg taggccggat aagacgtggc aagcgtcgca
    41041 tccggcaatg tgcttcaact agcgatggag cacatcttaa cccacgattt ccagtacctg
    41101 ctccggtgga cgaccaatcc gcgctttgcc attcgcgacc acaatcgggc gctccattag
    41161 cttcggatta tccaccattg cctgaatcag cgcctcttcg ctaagcgaac tgtcagccag
    41221 gttcagttct ttataaagat cctctttctg gcgcatcaat tcacgggcgc tattcatccc
    41281 cagcattttc agcaaatcgc gcagcgttgc cgcatcggcg ggtgtctcaa ggtagagtac
    41341 cacttctggc tccacgccgt tttctttcag caggttcagc gtttcccggc tctttgaaca
    41401 gcgtgggtta tggtagattt ttacctgttt ggtcattgtt cttcctttaa tgcgaattac
    41461 atcttggtat aaggcttaaa gcgttcctgc agctggcgca actggtcgat gcgcgcatcg
    41521 taacgcgctt gttgcaggct gcctaatttc acctgcgaac tggcgctact caacagcgaa
    41581 atggcctgat cgagtcgtcc ggcgagcgca taaccttctg ctcgcgcagc cagctcctga
    41641 tcgcggttat ttagcgcggc ttccgcctgt gccagtaaat cccagccgtt gctgtcatct
    41701 ttattattaa aggtgtagcg attcagaata ttggccgctt cttgtggttg accgccttgc
    41761 agataagcgt tcgccaggtt gagctgcaac accggattgg tgcgtaaatc gcgggcattt
    41821 ttcagacgat tgatcgcctc attggctttg ttttgcccaa gatcgatatc agtagccaga
    41881 tcgagatacc atgcgttgcc aggttctgcc gccagtaacg gttgcagcgt tttacgcgcc
    41941 tcgtcgtatt tattggcttc catcgcctgt aaagcacgac catattgtgc tgctcgttgc
    42001 tgacgaacgt ttcctttcgc ccattcatcc agcaaatcac tggtgagctg gttacgtccg
    42061 gaattataca tccccagtgt gcgcgctttc gccagataga aatcttccga cgactgcacc
    42121 accatcgggc gcatctgatt agcacggttg cgggcatctg ccagacgact ttccggcaac
    42181 gggtgagtca ataaaatttc cggcggacgc gaggagtaac gcgcctgatc gagtaatttt
    42241 tcgaggaagg ttggcatcgc ctgcggatcg aatcccgagc gttgcagcac ctgaatacca
    42301 atgcggtccg cttcctgttc attttgctgg gtgaaactga tcatcccctg acgcgttccc
    42361 gccagtgtac cggtcagcgc cgccatcccc gcctgcggac tggccatcgc cagtaaaata
    42421 gaacctaacg cgccgaccca ggtcagcggc gcgctgcgct gctgatcttc catcgctcgc
    42481 gccaggtgac gttgggtgac gtgggagatt tcgtgcgcca taactgaagc cagttgactt
    42541 tcgttatcgg aataacggaa cagggcagag tgcagcacca cgttgccgcc aaagaaagca
    42601 aaggcgttaa tttcgtcgtt gttgatcaga aagaaatgaa acggtgtctt aaccgaattg
    42661 gcatgcgaaa ccagacgcat ccccagcgaa ttaatatatt gcgttaacag cgggtcatta
    42721 attaacggcg cgctgccgcg tagctggcgg acataatagt cgcccatctg catttcctga
    42781 ccaatggaaa gcgtgcttcc tgcggaggtt cccatatccg gcaaggtgtc tgcgctgtcg
    42841 gcaaacgccg gggctacctg accaatggtc atagcagcaa tgagggttgc aaccaggttt
    42901 tttttcaact gcctgaacat aacctctatc ctgtatttct gaagatagtc atttgaccga
    42961 tactgttaaa tatcgttccc accgcgccgc ctttgagtgt aacggcgaag attagcccac
    43021 tcaaatgact tttcagtttc ctgaatccgt cataaaaagc gaagtctttt ttgtgacatt
    43081 tcgatacaat tgcggatcgc aatcccgctc ttgaggttca gggaaggtct ttatgctcga
    43141 aatgttgatg caatggtatc gccgccgttt tagcgacccg gaagcgattg ccttgctggt
    43201 tattttagtt gccggatttg gcattatctt tttctttagt ggcctgcttg ctccgttgct
    43261 ggtggctatt gtgctggcct atttgctgga atggccaact gtgcgcctgc aatctattgg
    43321 ctgctcccgc cgctgggcga cgtcgattgt attggtggtt tttgtcggta tattgctact
    43381 gatggcgttc gtggtactgc ctatcgcctg gcaacagggc atctacttaa ttcgcgatat
    43441 gccggggatg ctcaataagc tttctgactt tgccgccacg ttgccgcgcc gctatccggc
    43501 gttaatggac gcgggcatta ttgatgcaat ggccgaaaat atgcgcagtc ggatgctgac
    43561 catgggcgat tcggtggtga aaatttccct cgcctcgctg gtcggtttgc tgaccattgc
    43621 cgtctatctg gtgctggtgc cattgatggt cttcttcctg ctgaaagaca aagagcagat
    43681 gctgaacgcc gttcgtcggg tgctgccgcg caaccgtgga ctggcaggac aggtgtggaa
    43741 ggagatgaat caacaaatca ccaactatat ccgcggcaaa gtgctggaga tgatcgtggt
    43801 ggggatcgcc acctggctgg ggttcttgct ctttgggctg aactattcgc tgctgctggc
    43861 ggtgctggtc ggcttctcgg ttcttattcc gtacattggc gcatttgtgg tgaccattcc
    43921 ggtggttggc gtggcgctat tccagtttgg cgcaggcacg gaattctgga gctgcttcgc
    43981 ggtgtatctg attattcagg cgctggacgg caacctgcta gtaccggtgt tgttctccga
    44041 agcggttaac ctgcatccgc tggtgattat tttatcggtg gtgatcttcg gtggtttgtg
    44101 gggattctgg ggcgtattct tcgccattcc attggcgacg ctgatcaaag ccgtgatcca
    44161 cgcctggccc gatgggcaaa tcgcgcaaga ataatcttca gcccgacgaa ctgtttcgcc
    44221 gggctgattt ttcaggattc ctgacgtaaa taaatagccc gataacacat actcaccagc
    44281 actgcaccgc cgataatatt acccagcatc accgggagca gattggcggt aataaaatgg
    44341 ctgaccgtca gtgccggaaa attgtctgcg ctactgtgcg ccagttgcca gaaggaggta
    44401 ggggcgaaat ggcgaatggc aatggcgaac ggaatcacaa acaaattggc gatacagtgc
    44461 tcaaagccac tggcgacaaa cagggtgatg ggcaaaatca tggcgacgat tttgtcgcat
    44521 aacgaacgcc cgcaataact catccacagc gccaggcaaa ccatcagatt gcacataatg
    44581 ccgaggctga cagattcagt aaatgtatga tgcattttgc cctcggcgca gtgtaaaacc
    44641 gcgactcccc acatcgcgtt ttcactcatc accagcccgg aaaaccagat taacaaactg
    44701 aaacaggcaa tacctgccag attaccgcag gccaccagaa gtgcgttaat cagccaagtt
    44761 cgccaactaa taacgccccg actttttgcc attaccgtca ttaccgacga ggtgaacagg
    44821 ttggtgccgc aaaccgccag caaaataaag ccaagtgtaa agcaaaggcc gcccaccaga
    44881 tgggttaatg cctgtgaaga cggggtatcg gcgataacgg aaaggtaaaa agtaaatccg
    44941 atcgccataa acacgccagc ggacatcgcc attaaaaaga ccgacacttt actgcgggca
    45001 attttatggg cggcaatccg ttcagcgatt gccgcttttc tggcgctcaa ctgcaagtcg
    45061 aagagagttt gtttctcatg catactcctg caaaagcaga ttacaacacc tcgcgaaccg
    45121 agattcccag acgctgcatt cgtgacagca gcgtggtgcg cttcatccct aatcgtgtcg
    45181 ccgcgccacg gggcccggca acaatgccat tggtttctcg caatacctga acaatgcgct
    45241 gacgttcttc ttcgtcattc tctggcgtcg tcgggtgcag caactgcgcc attgaactgc
    45301 gaagcgctga atcttcattt agcgtcggca gtaaacggct ttgtcggaca ttaagatgta
    45361 aattcagact gttaccgcga gtcagcagta ccgctcgctc aatcacgttt tccagctcgc
    45421 gcacgttgcc cggccagtcc cacgacatca actggcgtag cgcctcggtc gggatggcgt
    45481 caattgaacg attcatatgg cgcgccattt tttgcgtgaa atgctttgct aaaagaggga
    45541 tatcttccgg acggtcgcgc agcggcggca attccagtgg gaagacattc aggcgataaa
    45601 agagatcgct gcgaaactgg cgatcttcaa ccatttgcca caaatcacgg ttggtggcgg
    45661 caatgactct gacatttacc gggatcgttc tactcccgcc gagacgctca atctcccgtt
    45721 cctgcaatac gcgcagcagt ttaggctgaa gttctaacgg cagatcgcca atttcatcga
    45781 gaaacaacgt gccgccatcg gcaatttcaa aacgaccacg atgggtatta atcgcaccag
    45841 taaacgcccc tttgtcatga ccgaataact cactttccag aagactggcg gggatggcag
    45901 cgcagttgat tttgaccagc ggcttgtcgc gtcgcgggct aagttgatgg atcgctctgg
    45961 cgatcacctc tttgccggtt ccggtttcac cgcaaatcaa caccgtactg tcgctcttcg
    46021 ccacaatatc tacctgctgg agtaggtctt ccattgcctg gctttgatag ataatgtcac
    46081 cgatgcccag attcgaaagg agctgctcgc taagctggtg gttttcttgc tgcaaacttt
    46141 cctgcaaatc ggtcatgcta cgccaggcat cggcattgcc aacggcaata gcgatgcgat
    46201 cggctatgtg ttgtagtagc tggcagtttt cctcactaaa gagagtggaa gaggtatgcg
    46261 ccagcaacaa tgcacccggt gtatggttgc caaaggtaag cggtataagg agcgcagatt
    46321 cgcagccgtt agatgcaagt aagagtaata acgggtcgcg ttgccagaga aacagatcgt
    46381 ctgcacggtg cgtcaaggtc ggttgtcggg tttgtagcgt ttgtgtcagc aatacactgt
    46441 cgccaggcat actgcgtggc agacacgcac aatgtgaggc agaaagatcg ctgcaccaca
    46501 ggctaaactt ctcgttcttt cgatgatcgc ccagtaccat actgactgaa gccagaccga
    46561 aaaaatgatg gatctcacga gcgacgtcag cgatcagatc gtcgagatca aaatgtgaca
    46621 gcactgcatt agtaatatcc accagtaccc gatgatgatc gcgatctttc tccagcaaac
    46681 gcagcgccac ctgctgttct tcatcattga gccatgattg cagaaccggg gtgattatcc
    46741 gcgccagcca gttaaacagt gtgcaatcct ggggagtaaa acggctgccg tgggtacgaa
    46801 taaagcgtag ctccgccatt ccactactgc cgagaaggac ggcgtgacgc gaataatcga
    46861 ccatctcgcc ctgatactct gttgttgccg tcacatcgct gccatcatca tggtagcggt
    46921 aatagaagtt gcttttgaga acatgcagtt caataagttg taccgggctg aacggcggcg
    46981 gcatcgggat gaaggcgcgt aataaagacg ccttaccgtg tttctgtgct aaccgctccg
    47041 cgagcattcc gtttaccgct tcaattgtta ttccttgtgg cggggcaaac atcgcctcgt
    47101 ctgacatagc catcaacagt ccttattaat gagaaatctt tctcacgcaa tttttctggc
    47161 catcagatag atggccggtt cctgcacaat ttcatccaac agatgaatga attgaatgct
    47221 acgcgcctgc cacacagacg tcgcgtcatc tgccagtggc gcgaatgccc gggtcaacag
    47281 tgcggtgtgg ctggcgtcaa taacaatctc accaaaagtc atcaccccca gcatcttacg
    47341 tcgggcttgc tcctcttcga ccagtgcaag ccaatcttcg tattccgtca gtgggcagcg
    47401 gaaggcgaca ttcagacaat caatcacgcc aacgtgatga ccgatagcca gcgagtaata
    47461 cattacctgg gagctgtgtt ccggcatctc gtcgctactg gcgacaaact tttttcgcag
    47521 cgtccagaaa acaatttccc cgcactcctc agccatgtaa cccccggaga acgcgaaaga
    47581 gttgctgaac gatactattg agacgcggat cgtcagcttc gcgcaaccag gtgttaaccc
    47641 gatttcctgt aggatcgtcg cttaaatggc gcaggagccg atcgcaaatt tctcgcccct
    47701 gacgatagcc cgccagctgc cgcgcttctc gctcaatggc gatacgctgt gatggcggga
    47761 tctgcggcca cagcggttgc atggtcaccc cggtgggatc gcgataatcc acagcgtgaa
    47821 tcttctgttg cagcaaaccg agcgccacgg cgaaaccgtg aatggtggcg gccggtgttg
    47881 gcgggcagcc ggggatccaa acatcaatgg ggacaatggt gtcgctgccg ccccagacgc
    47941 tgtagagatc gtggaaaatc cgccgccggc accgcacgcg ccgtaggaaa cacagatttt
    48001 atgatcgggg gcagactcat acgcccgaag tgcaggcata cgcatcgccc gggtgagtgc
    48061 gccagtaaat aacaaaatat cggcgtgacg cggtgatgaa acaaccttaa tgccaaaacg
    48121 ttctgcgtcg aatactggtg taatggcggc aaaaatttcg atttcacagg cgttgcagcc
    48181 accgcaatcg acgcgataaa cgtaggctga gcgacggata tcctgtagca aatgccgctt
    48241 catcttttgc gtttgctcgt ccagcgtgat gggctggctg acatattgcg taagcactgg
    48301 actcatagct gctccttagc caccagcggt acatctgtat cgtcgttgag cagcgtcgcg
    48361 cgttgtttgc actccgggca gacgctcgct tgcgcccata acatttcgcg gttttgtggc
    48421 gcattttgtt gctgtgccaa caattcagca gccagtgcga cggttttttg ctgggcaaac
    48481 gggcgttcgc aacggctgca acgttgtaga tggaacgtcg cgcgggtata gagatcggct
    48541 ttattggtga cggtcagttc aaagttattg gtaagctgat ggctctggtc gggcacactt
    48601 cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg cgcgaatttt
    48661 gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg ccgcaggcaa
    48721 tacattggct gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc tccagtggcg
    48781 cgaagggata tttcaccgtc gcggttccgg cgcgcataat agttttcagt aacttcagca
    48841 tcttctgtcc ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt tcgatctctt
    48901 tatacggcac ggtttttgac tggcgcttgc gcacatctac cagcgtcaca cggtcggtac
    48961 aggagtagca gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg gtattgccgc
    49021 gcagcatgta acgcaacacc ggccagttgg cgtaggtggc ggcacggcag cgccagcgga
    49081 acaatttttg gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt ggcgcttcaa
    49141 caaagcccag cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc agtggggtat
    49201 ccggcatgtt gtcgagagca aattctagca ttgccagcga atcaaacgtc tctttgacac
    49261 ggaccatcac gcgggagaaa acatcgccgc cggtaaaggt aaacagtgtt tttggaatat
    49321 tgccgtagtc ggcgtagggg tgatcaaagc gcaaatcacg ggcgatttgt cggtcgagaa
    49381 tgccaatgcc ctgagtgcgt tgttccatat tcggcgtagc aagcagcatc tctaccagct
    49441 ccgacacgtc ggcgcgcatc tcgcgcacca gtttcagcgt ttgcagacgt tgctctttga
    49501 gaatatcgcg gcgaacacca ccaatcagat tcagaccgta ggttttacgc gacccgatca
    49561 gcaattccgc catcgtcatc gacttttccc gcacgcggaa aaattgcata aagccggtat
    49621 caaaaccaac gaaatggcag gagaggccaa ggttaagcaa atgactgtgt agccgttcga
    49681 cttccagcag aatcgagcga atagtatgtg ctcgttgcgg cacctcaatc cccagtgcat
    49741 tttcaaccga gttggtatag gccacactgt gggcaaaacc gcaaatccca cacacgcggt
    49801 ccgataagaa ggtcacttcg ttgtagccca tccgcgtttc tgccagtttc tccatgccgc
    49861 gatggacata aaacaggcgg taatcagcat cgacaatttg ctcgccatcc acaaacaagc
    49921 ggaagtgacc cagttcatcg gaggtgatat gcagcgggcc gacagggatc acctgcgcat
    49981 cgctgttgcc ctcattgata aacggatacg tttcggaatc agtcgtcggt tcagggcgca
    50041 gtcgataatc catcgcatct ttgcgcagcg gatgcatatc ttccggccag tcatcgggca
    50101 acaccaggcg acgctgatcc ggcaggccaa ccggaatcag cccgtacata tcgcgaattt
    50161 ctcgctcgcc ccagaccgcg gcagggacgc gcggtgtgac tgacggaaac tcccgactgt
    50221 cggcatcgac cagtgccttc accacgatcc agcatttttc ggccccttcc attgaaaggg
    50281 cataataaac cgcgtaatga ccgttaagtg tccgctcgtc gttgccaaac aggactggaa
    50341 gccagccatc atgttgataa taaagataat gtacaacgtc aggcagcaga ttgattttca
    50401 ccgtaatggt gacctgttca ggcgtttgtc gctcttcatc cagcaccgcg ccggggaact
    50461 gcgttttcag ggcggcgaga atcgcttcgc cacgatttga cgatgaatta acgttcactg
    50521 tgtttctccg aaacagatgc ggtgcctgaa ggcaaaaaat catgccaggt gctacgttgt
    50581 gcaggcaggt cgtgcgtccc tgagaggact atagtggaag cgcccgccag gatcctgatg
    50641 acaggttgtg gaatatgcgt tcccatcgcc agcatcatga ccagcagaat caccattggc
    50701 gaggtggtca accagccgag atcaccccgg ttaacggcct gcggcggttt cgccattaac
    50761 acccgcgctg ccatccgtac caggcccgcc agcaccagcg ttaacagcaa taacagcagg
    50821 acgataatca gcaggtgatt acgtgccagt ccggcggtaa tggtcataaa ttcgctaaga
    50881 aaaatgttga agggcggcat ccctgccagc gccagcgcca gcgcaccgcc gccaaacagc
    50941 acggcggtaa atggcatgat tttgagcatc ccacagacga cgttgagatc gcgcgtgccg
    51001 tacttgagca gtacattgcc ggaaccgcag aacagcagcg tttttgccag actgtggttt
    51061 aaggtgtgca gcagcgcggc aaaaattccc agtggcccgc caatgcctag cgccaccgcg
    51121 accagtccca tgttctccac gctggagtac gccagcagac gcttaatgtc ccgctgtacc
    51181 agaatgaaaa atgccgccac ggcaaccgac aacatgccga agatgagcaa caaccggttg
    51241 gggaaatcgc tgccgatggc ctggcagata atgatgtaat agcgaattag caccaacagc
    51301 gcgcagttca gcaatacggc ggagagcagg gcgctgaccg gactcggtgc ttcactgtga
    51361 gcatccggca gccaggcgtg catgggaaat agcccggttt tggtgccaaa gccaattagc
    51421 acaaacacaa aggccaacag cattaatgtt gggtcaagca aggacgattg cttaagaacc
    51481 tcgctccaga atatcgccat ttctgcctgc ggcataacgc tggcgacgtt ggcgtatacc
    51541 agcacggtac cgaacagacc aaaagcgaca ccaacagtac aaataatgat gtacttccat
    51601 gctgcttcca gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg
    51661 gtggcttcga tcgccgccca catcacaatc aggttattgc tggtaacaac cagcagcatg
    51721 gtaaacaaaa acagatggaa gaaaccgtag taatcgcaca gcgtaacggg ggaaagctcg
    51781 ccgtgtgcca cttcatgacg catgtagcca atcgagtaaa caccggtgag aaagccaatc
    51841 acaccaagaa tggcgaggaa caaaccgccc agaccatcaa tatgaagcca cagtcccgca
    51901 gcgaatattt ctcctgcatc agcggcagtt tggaccaccc agagtgccag aatcagcagc
    51961 agtgtgatcc ctaagctatg taatacagtc accgtacaag tcgcagagag cccccgtttc
    52021 cggcaggcaa aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc
    52081 acagaataac tcatctcatt atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc
    52141 gtgggtacgc catatttttc ttgccagtaa caccatcaca atgacggcga agatggcgtc
    52201 ggtagcgata cctatttcca ccagttccgg tgctcgccag gcaagaagcg ccagcaccag
    52261 atgggagccg ttttccatca ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct
    52321 gacaatgcac agcaggccaa gcagaaaatg acctaacgct accgccagcg ccggtttcag
    52381 cccggtagcc atcggtagct tcacgggctg aacgacaaat gcgcaaagca ggacaattaa
    52441 cgccgcgagc agtgccatca ttgccggacc gaataacgct ttttccggga tgttctgggg
    52501 aatatttcgt gcagcgtaag tcatgattaa cggtaccagc agcactttgg tgataaaggc
    52561 gctggcggac cagatcagca gttgctctgc ggcgaacagg cacgagagag tggcgaaaat
    52621 agacaccagc accagcgact ggcaggcgta aaatccgcat gacaggcgat agcttttgac
    52681 gctaatcaca aacagcgatg tcagcatcat cagtcccgcc agattattta cgatcataga
    52741 accggtcatt catcatctcc ttactgcaac caggtcgcgg cgattacgct ggaaatcagc
    52801 gacatcacaa tcagtacaat caacaccagg cgcattgacc ctggcagcgg tgcggcatcg
    52861 gcgacggcct cgctcggttt gccaggcaca acgcgaccaa accagcgaat aaaccaggcg
    52921 aaactggcga ccgattcaat catcagcaga atcatggcgg gcagcaggat ccagtactcc
    52981 actgacaacg caaaaccggc agcaaacagc gggaatttac tgaagaagcc gttgaacggc
    53041 ggcacgccgg taatcgccag cgctgccacg cagaaaccca cgcctggcaa cggcagggtg
    53101 tgcaatacgc cacgcagacg cggcaacaag cgcgtgccgc agctgtaact cagcgcacct
    53161 gctacaagga aaaacaggct tttagcgaac gcgtggttga cgatgtaggc gatgctgccc
    53221 tccagtgcca gccgcccact ttcaccatcg atgcggcgtg gagataggcg ctgatcggtg
    53281 ttggcgcttc cattgcatcc ggtagccacg cttgcatcgg tagctgggcc gatttccccc
    53341 acgcggcaaa caggatgccg ccataaacca gataacgtgc gtcgccatgt aactcgctca
    53401 tcgcgctaag cacaaacgtt ccggtctgca aaaacagcgt ggcggcggca agatacaacc
    53461 ccaacgaacc gatatgggtg ataagcaacg cttttagtgc tgaacgctgc gctttatcgc
    53521 tctggtaata actgatcaac gcccaggagc agccgcccgt aatttcaaaa aacaacaact
    53581 gaccgagcag cgtcgaggag agtaccagtc ccgccatcgc gccgataaac accagcaaaa
    53641 atgcgtaata acgattcgtg ccgttatgcg ggtgttcgcg atttttatcc gtcagataac
    53701 ccgtggagta gatcgtgacc agcaaaccga gaaacaccac cacaaacaga atcagcgtac
    53761 tcacgcgatc aatgaccagg ccaaacaatg ccacatcccc cacgttgacc aacgtcaacg
    53821 tgacggcaac tttatcggcc tgataaaacg cggagatcag cgacaacatg cacagcgtgg
    53881 tcagtgcggc gaacaaaacc cccattcggc ggcccgacgt tgtggcgaaa acgaaacgac
    53941 cagtgcgccg ataaaaggca gcattaacgt cgtcagagca agattttcca tattccgtca
    54001 gtgctcctta cagaccggtt aaccagaaga cccatgcaag cgcagcaagg ctgaagccaa
    54061 gccaggtcac atggtgaatg agtaaaaaac gcccgcgtgc cagcgtgttt tctgcgattg
    54121 aggccagtac aaaaatcagc aaaaccttga gcagcgtaac gacaagtgaa gtcagcaggc
    54181 aggcgagaga aagttcttgc gcgcgcccaa agggcagaaa cagggccaca aacagtgctg
    54241 ccatcacgac ctgtttcagc cccagccccc atttcgctag cgccagcccg gcaccggaat
    54301 attcggtcag cgggccttcc tgtaattcct gttctgcttc agcaacatca aagggaattt
    54361 ttcccatctc aatgaagcag gcaaaaccac aggccagtaa cgccagtacg gtggttagcg
    54421 gcgagttcca gcccgttgcc agcgtattgc tgatcatctc gatatgcgtg gaacctgcta
    54481 tcagcgccaa taccagcagt gagagaatga gcatgggttc gaccagaatg ccgagcgtca
    54541 actcgcgact ggcaccgact cccgcaaacg ggcttccggt atccagcccg gaaagagcaa
    54601 agaaaaaacg aaacagggca agaagataga taagggtgat cagatcgccg ccgcccgcaa
    54661 aaggggaaac ggtaataaaa agtggtaggg ccatcgccag caccagcatg ctgctgatta
    54721 atacccacgg catcaggcgg aacatcagac ctgaagatat aggcgcaact tcctggcgtt
    54781 taaacagttt gtggatatcg cgataatcct gccagatccc cggcccgcgg cgggagtgca
    54841 tacgcgcgcg tatctgccgg gaaatacccg taaaaagcgg ggttagcatc agcagaatca
    54901 cggcctgtgc taacgcaaaa atgaccagat atccgtcgta aagagtttgt ctcatggtga
    54961 tttccttaga cggcaatagc gattagcagc acaaccagtg cggcgaccac atacaggcaa
    55021 tagagacgaa agtcgccgct ttgcagatgc tgtatttctt tggccagccg ttgggtggcg
    55081 ctcacgatgg ggcggatcac ccgctcatcc cagaagggtt ctgtgctctg agccctggcg
    55141 gtgacgtgcg caagaccttt atttagcctc agcgtagggt cgaactgttg tcgtagacga
    55201 aatagcgcag aaaagaccac acgcagcgac tgcatcacgc cattgcctga cggggccatc
    55261 gcattttccc agccgtagcc gcatgcccag gcatctcctg tgcgacgaaa cgctgcgcgg
    55321 cgcgaacgac accacagcca gtaaaggcca ggcagcaaag gcatcgccag tagtaacagc
    55381 aacagtaatg agggggtgac ctgtgtatga aacgtgccgg gtacaagtgc tattccgctg
    55441 gcgacagtga cgggaggggt attggtaaac gcatgggcaa tatgcattat cttcggtgcc
    55501 agccaactgg cactaagcgc aattaatacg cagagtgccg cgagcagtag catggcgacg
    55561 atcatcgtat ttggcacttc ctgtgcctct tcagcgtgtg tactgcgcgg cgcaccacag
    55621 aaagtaatac cgtacatttt tacgaagcac attactgcca gcccaccagt gactgccagc
    55681 attaccatag caataggacc cgcaagttgt agcgctacgg cttccacacg acttagtgag
    55741 aacagcgact gccaggtgta ccattcgctg ataaaaccat tcagcggagg aatggctgat
    55801 atcgcgaggc aaccaatcag gcatgctgcg gctgtccacg gcatccgttt cgccagtgcc
    55861 cccatttttt ccatgtcgtg ggtatgcaaa cgcgaaataa tcgcgcccgc gccgagaaat
    55921 agcagccctt tgaacagcgc atggttgagc agatgaaaca gtgcgccgag cagtccaacc
    55981 acggtgagca gcgggtcgtg cagtgacaga ccgaccatcg ccacaccgac tgccagcaaa
    56041 ataatgccga cgttttcgac ggtactccag gccagcagcc gtttgatatc ctgttccgcc
    56101 agcgcataca gcacgcccag gagcgcggag attgcgccga tcgccatcac cagaatgccc
    56161 caccacagcg gcaaacccgt ttgcgccagc aggtccatcg ctactttcag gatgccgaaa
    56221 ataccgattt tgaccattac gccagacatc aacgccgaag cgtgcgatgg tgcggcaggg
    56281 tgagcgcgcg gcaaccagct gtgcaacggc atcatcccgg ctttcgcgcc aaaaccgaaa
    56341 aaggccagca ggaacaccgc cgacgccagc cccggagaaa gtgaaagcgt gcggaaactg
    56401 gcaaaatcga ggctgcagct ttcgcgcccc atcagcaaga aggcgatcat aatcagcacc
    56461 gatccggcgt gggcgataaa aaagtagagc atgccagcgt tgatcgacgt tttatcctgc
    56521 ctggcaatga ccagaaacca ggaagacagc gacatcattt caaacagcac gatgaaccaa
    56581 aaagcgttgt ccatcaccag cagggcaacc atcgatgcga tgaaaagatt cataaagaag
    56641 cccatcgccg ccgcgccttt gccctcgtat tcgcgcatat aagtcaatga atagagcgaa
    56701 caaaccacca ccagcaagga gataaccagc accataaatg ccgagaggct atccattcgc
    56761 agggtaaaat cggcaaagtc aaaaggggtg gcaaaacggg cgactaaagt tacaccagca
    56821 tgtaattgcg taattccgct gatgatgcca atcacgccgc ccaccagcga tgtgatgccg
    56881 gaaagcttaa tagccagtct gtccagaccg agtaaaaaca gcgaagccag actggcaaac
    56941 agatagagga tcagcgacca ggttaataat tgcagggcat ccattagcgt tgctcctgag
    57001 tgagggaaag gggatcctcc ccgccattgg caaaccagct tgcggcaagg cgctgttttt
    57061 ctttcatctg tcgttgcagg ctatcgtcgg tgatcagccg taacgcctga ttcgggcaag
    57121 cgcgaacgca ggccggacct tctggcaaga aatcacacag gtcgcatttc accgcgacgg
    57181 tctggacacc cggttcccag cgcagcaatg catgttgggg cggcacgttt tcttcgccgt
    57241 ctttgagtga gccttcagcc tgaaaaacat attgcgcagg ggcattcacc ggacggcttc
    57301 ctgaagcgct gattgcgcca aatgggcaga ccacggcgca aagcttgcag ccaatacaga
    57361 ggctttcgtt gagttggatc gcatcatccc tctgggagat ggcattaacc gggcagacct
    57421 gcaggcaagg ggcttcctca cagtgatgac acacgacagg ggcagtgagt gttgacgtct
    57481 tcgccagggc caggcgcggg tgttgctgta aaccttgcgt tttatgcacg tccgaacagg
    57541 cagcgagaca ggtattacat cctgtacacc acagtggttc ggccaccaca aagcggttca
    57601 tgtcggctcc ttgaaaatga tatgaaacat tgcatttcat attgcataaa ggatgccatt
    57661 ctgattaact caatatattc aataagttat gtattgactg gattctgaac ggtacaaaaa
    57721 tgatggtcat taacgttcag caaaaaatgg attcgacacc cgtgtcgcag gggaaagcga
    57781 gaatgtgatc ttgatcacga gaagtgaggt atgctggccg caagcgcagc cagcacggaa
    57841 tggagcaaag taatcaggcg tgttctttca gccagttcag cacaacgtcg tggtgattgc
    57901 tggttttgaa atcgtcaaag acatgttcga ttttgccatc agcgtcaatc aggaagctga
    57961 tgcgatgaat gccatcgtag gttttgccca tgaaggactt ttcaccccag acgccgaatt
    58021 gttcgcacac ctggtggtcc tcatcagaca ggagcgtaaa gttaagcagc tctttttccg
    58081 caaaacggga gagtttttcg ggtttatcgg tgctgatacc cagcacatca acgcccgctt
    58141 ttttcaactc atccatgtta tcgcgtaagc cgcaggcctg tacggtacag ccgggggtca
    58201 tggctttcgg gtagaaataa accagaacac gctgtccctg gaagtcggtc aaattaactt
    58261 gttctccgtc ttgatccggc aagctaaatt tcggtgcgat atcaccggct ttcagtggat
    58321 tcattactta actccatcct gttcatcatg ttgggaataa ttgacgacgt taatactgcc
    58381 ttgtgcattg agttctgtac atagggcttt gaacgcttgc tcaatatttg ctgcgtccgc
    58441 agatgcgggg ctgtgggcgg ttatctgaat atgcaactgc gcagcccgtt cattttcagc
    58501 aggttgcgtg cgcgacacca gctccgcaat gttcatatga tgcgcgtcga aaagtgctgt
    58561 gaagcgttca attaaatgcg gggagtctgc cacatcgacc tgaacccaga cagatgctgg
    58621 cattggcgga cgcggacgcg ccgtcgtgcg cttcatcacg attaaaagat ccagttcggc
    58681 acctttcaac ggtaacgttg attcaatcag agtaatggca ttccatgaac cggaaagcag
    58741 cataataaac gtgaactctt ctcccagcat cgccaggcga ctgtcttcaa tattacagcc
    58801 gcaactactg acatgacggg tgatggtgtt cacaattcca gggcgatcgg cacccaacgc
    58861 agtgatcacc agataatgtt gcgatgacag tgtcaaactg gttattcctt taaggggtga
    58921 gttgttctta aggaaagcat aaaaaaaaca tgcatacaac aatcagaacg gttctgtctg
    58981 cttgctttta atgccatacc aaacgtacca ttgagacact tgtttgcaca gaggatggcc
    59041 catgttcacg ggaagtattg tcgcgattgt tactccgatg gatgaaaaag gtaatgtctg
    59101 tcgggctagc ttgaaaaaac tgattgatta tcatgtcgcc agcggtactt cggcgatcgt
    59161 ttctgttggc accactggcg agtccgctac cttaaatcat gacgaacatg ctgatgtggt
    59221 gatgatgacg ctggagctgg ctgatgggcg cattccggta attgccggga ccggcgctaa
    59281 cgctactgcg gaagccatta gcctgacgca gcgcttcaat gacagtggta tcgtcggctg
    59341 cctgacggta accccttatt acaatcgtcc gtcgcaagaa ggtttgtatc agcatttcaa
    59401 agccatcgct gagcatactg acctgccgca aattctgtat aatgtgccgt cccgtactgg
    59461 ctgcgatctg ctcccggaaa cggtgggccg tctggcgaaa gtaaaaaata ttatcggaat
    59521 caaagaggca acagggaact taacgcgtgt aaaccagatc aaagagctgg tttcagatga
    59581 ttttgttctg ctgagcggcg atgatgcgag cgcgctggac ttcatgcaat tgggcggtca
    59641 tggggttatt tccgttacgg ctaacgtcgc agcgcgtgat atggcccaga tgtgcaaact
    59701 ggcagcagaa gggcattttg ccgaggcacg cgttattaat cagcgtctga tgccattaca
    59761 caacaaacta tttgtcgaac ccaatccaat cccggtgaaa tgggcatgta aggaactggg
    59821 tcttgtggcg accgatacgc tgcgcctgcc aatgacacca atcaccgaca gtggtcgtga
    59881 gacggtcaga gcggcgctta agcatgccgg tttgctgtaa agtttaggga gatttgatgg
    59941 cttactctgt tcaaaagtcg cgcctggcaa aggttgcggg tgtttcgctt gttttattac
    60001 tcgctgcctg tagttctgac tcacgctata agcgtcaggt cagtggtgat gaagcctacc
    60061 tggaagcggc accgcttgcg gagcttcatg ccccggctgg aatgattttg ccggtgacct
    60121 ccggtgatta tgcaatcccg gtgaccaacg gtagtggtgc tgtcggtaag gcgctggaca
    60181 ttcgtccacc agcccagccg ctggcactgg tttctggcgc gcgtacccag ttcacgggcg
    60241 ataccgcttc attgctggtg gaaaatggtc gtggcaatac tctgtggccg caggtggtta
    60301 gcgtgctgca ggcgaaaaac tacaccatca cccaacgtga tgatgctggt cagacactga
    60361 ccaccgattg ggtacaatgg aaccgtctgg acgaagacga gcagtatcgt ggtcgttatc
    60421 aaatctctgt taagccgcag ggttatcagc aggcggttac ggttaaactg ctgaacctgg
    60481 aacaggcggg caaaccggtt gcagacgcgg cttccatgca gcgttacagc acggagatga
    60541 tgaacgttat ttccgccggt ctggataaat ctgccactga cgccgcgaac gctgcgcaaa
    60601 atcgtgcctc caccactatg gacgtacaaa gtgcagctga tgacaccggt ttaccaatgc
    60661 tggtcgtacg cgggccgttc aatgtggttt ggcaacgtct gccagcggcg ctggaaaaag
    60721 tgggcatgaa agtgaccgac agcacccgtt cgcagggcaa catggccgta acttataagc
    60781 cgctgtctga cagcgactgg caggaactgg gcgcgagcga tccaggcctg gcatccggtg
    60841 actataaact gcaggttggc gatttagata accgcagcag cctacagttc atcgatccga
    60901 aaggtcatac tctgactcag agtcagaacg acgcgctggt agctgtcttc caggctgcgt
    60961 ttagcaagta aaaatacagg gctggaatca tccggccctt ttttctgata tgatacgcaa
    61021 acgtgtgcgt cggcaggaaa acgcgatttt agcggtaatt cgcacgaaat ttgtttgtcg
    61081 gacgtagttc ggataaggcg ttcacgccgc atccgacaaa acatccggca caccagacag
    61141 caaaagattt taaaacgtta attcacaccc aggagtgata aagatgcaaa agcaagctga
    61201 gttgtatcgt ggtaaagcga aaaccgtata cagcacggaa aacccggacc tgttggtgct
    61261 cgaattccgc aatgatacgt cagcagggga tggcgcgcgc attgagcagt ttgatcgcaa
    61321 aggtatggtg aacaacaagt tcaattactt cattatgagc aaactggctg aagcgggtat
    61381 cccgactcaa atggagcgtc tgctctccga taccgaatgt ctggtgaaaa agctggatat
    61441 ggtgccggtt gagtgtgtcg tgcgtaaccg tgctgctggc tctctggtga aacgtcttgg
    61501 aatcgaagaa ggtattgagc tgaacccgcc gctgttcgat ctgttcctga aaaacgacgc
    61561 catgcacgat ccgatggtca acgaatctta ctgcgaaacc tttggctggg tgagcaaaga
    61621 gaacctggcg cgtatgaaag agctgaccta caaagcgaac gacgtgctga aaaaactgtt
    61681 cgatgatgct ggtctgattc tggtcgactt caagctggag tttggcctgt acaaaggtga
    61741 agtggtactg ggcgatgagt tctccccgga cggcagccgc ctgtgggaca aagaaacact
    61801 ggagaaaatg gacaaagacc gtttccgcca gagcctcggt ggcctgatcg aagcctatga
    61861 agccgtcgcc cgccgcctgg gtgtacagct ggactgattt ttctggttgc ttgtttcttt
    61921 agccggaggg tactccggct aaatgtcttt gatacccccg ctacaatact tctcattatc
    61981 tccctttgac aggaaagtca taccaggcca atatgtatat acataacgta tatcccaaaa
    62041 agggggggct atggaaacta ctgtattttt aagtaatcgc agtcaggccg tcagattacc
    62101 gaaagcggtc gctctgccag aagacgttaa gaaagtagaa attattgcga ttggtcgtac
    62161 aagaatcatc actcccgccg gtgagtcctg ggacagttgg tttgatggcg aaaatgtgag
    62221 tgcggatttt atggatatcc gtgatcaacc cgcaatgcag gaaagggaat cattttgatg
    62281 ctgaaattca tgctggatac caatatctgt attttcacta ttaagaataa accagtacat
    62341 gtcagagagc ggtttaacct gaattctggc cggatgtgta taagttctgt aacattgatg
    62401 gagcttatct atggggcaga aaaaagtcag atgccagaac gcaatttggc ggttattgaa
    62461 ggtttcgtat ctcgtttggt agtgcttgat tatgatagtg ctgctgcgac gcataccggg
    62521 caaatcaggg ccgagcttgc ccggcaggga aggcctgttg gaccttttga ccagatgata
    62581 gcgggacatg ctcgcagtcg cggtttgatt gtggttacca acaatacccg agagtttgag
    62641 cgagtggcgg gaatcagact agaagactgg agctaacatt ctcttttact tctaacattc
    62701 gtaaattata taacgtggca tgaagcctta attcctccgc catttcatcc cgctttcctg
    62761 tggtaatctt cctctgaacc acctacgatc atttctataa tgaatatatt gttgaggtat
    62821 ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga agacaggcgc aacagctccg
    62881 gtggtccgtc tatgggcggt cccggttttc gcctgccaag cggtaaaggc gggctgattt
    62941 tactgttagt cgtgctggtt gcaggctact atggtgttga tttaaccggg ttaatgactg
    63001 gacagccggt ttcccaacaa caatcaacgc ggtccattag ccctaatgaa gacgaagctg
    63061 caaaattcac ctcggtgatt ctggcaacca cggaagacac ctggggacaa cagttcgaga
    63121 agatgggtaa gacctatcag caaccgaagc tggtcatgta ccgtggaatg acgcgcaccg
    63181 gctgcggggc tggtcagtcc attatggggc cgttctattg cccggcggat ggcacagttt
    63241 atatcgatct ctccttctat gatgacatga aagacaaact tggcgcggat ggcgattttg
    63301 cccaggggta cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg ttaggcatcg
    63361 agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac cgcttatctg
    63421 tgcgtatgga actccaggcc gactgttttg ccggtgtctg ggggcatagt atgcagcagc
    63481 aaggcgttct ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg
    63541 atgaccgttt acaacagcaa agtcaggggc gagtagtacc agatagtttc acacatggca
    63601 cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggcgat ccggcacaat
    63661 gcaatacttt tggtaaaagc atttaacttc cggggcaggg atggctgaac tgactgcgct
    63721 tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg
    63781 ggaagagggt tggtgttttg atcatgcgct taagttgcgt gatgccttac ctggcgactg
    63841 gctgtggatt tcgccgcagc cagatgctga aaaccactgt tcaccctcgg cgctacaaac
    63901 tttacttggg cgcgagttcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc
    63961 tgcctttgca gcacttagcg gaacgttgaa agcgggaagc tggctggttt tgttactccc
    64021 tgtatgggaa gagtgggaaa accaacctga tgccgattcg ctgcgctgga gtgattgccc
    64081 tgaccctatt gcgacgccgc attttgtcca gcatctcaaa cgcgtactta cggcggataa
    64141 cgatgctatc ctctggcggc aaaaccagcc gttctcgttg gcgcatttta ctccccgtac
    64201 tgactggcac cccgcgaccg gcgcaccaca accagaacaa cagcaactct tacagcagct
    64261 actgaccatg cctccgggcg tggcagcggt aacggctgcg cgtgggcgcg gtaagtcggc
    64321 gttggcaggg caactcattt ctcgtattgc gggcagtgcg attgtcaccg cgcccgcaaa
    64381 agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcaccgga
    64441 tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgacgaag ccgcagccat
    64501 acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac
    64561 ggtgcagggc tacgaaggca ccggacgtgg ttttttgctg aaattttgcg ctcgctttcc
    64621 gcatttacac cgttttgaac tgcaacagcc gatccgctgg gcacagggat gcccgctgga
    64681 aaaaatggtt agtgaggcac tggtttttga cgatgaaaac ttcacccata caccacaagg
    64741 caatatcgtc atttccgcat ttgaacaaac gttatggcga agcgagccag aaacgccgtt
    64801 aaaggtttat cagttattgt ctggtgcgca ctaccggact tcgccgctgg atttacgccg
    64861 gatgatggat gcaccagggc aacatttttt acaggcggct ggcgaaaacg agattgccgg
    64921 agcactgtgg ctggtggatg aggggggatt atctcaacaa ctcagtcagg cggtatgggc
    64981 aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggtagcaa
    65041 tccactggca gcgacattgc gtggacggcg ggtcagccgg atagcagttc atccggcgcg
    65101 tcagcgggaa ggcacagggc agcaacttat tgctggtgct ttgcaatata cgcgtgacct
    65161 cgactatctt tcggtgagtt ttggttacac cggggagtta tggcgtttct ggcaccgctg
    65221 cggttttgtg ctggtgcgga tgggtaatca tcgtgaagcc agcagcggtt gctatacggc
    65281 gatggcgctg ttaccgatga gtaatgcggg taaacaactg gctgaacgtg agcattaccg
    65341 tttacgtcgc gatgcgcaag ctctcgcaaa gtggaatggc gaaacgcttc ctgttgatcc
    65401 actaaacgat gccgtccttt ctgacgacga ctggcttgaa ctggccggtt ttgctttcgc
    65461 tcatcgtccg ctattaacat tgttaggttg cttattgcgt ctgctacaaa ccagtgaact
    65521 ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgtc agcgacgcgc agttatgtac
    65581 cacacttaaa ctttcaggcc gcaagatgtt actggtccgt cagcgggaag aggccgcgca
    65641 ggcgctgttc gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca taacgcaatg
    65701 gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa
    65761 aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc
    65821 cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg
    65881 caatggggga aataggtagc tggtttgcac cgctgtttcc tgatgcgctg gtggtgagtg
    65941 tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttca gtgcagggta
    66001 ttacggaaga taatcgccag gcgcgtgtgg atgccatcat gccgacgttt attgaaacgg
    66061 tacgctactg gcagaaacag agcggagtag gggctaacgc cacggcgcta attggtttct
    66121 cacaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt gcttcgcgcg
    66181 tcattgcctt taacggacgc tatgcgagcc tgccggagac cgcgtctaca gccacgacaa
    66241 ttcacttgat tcatggtggt gaagatccgg ttatcgatct ggcgcatgca gttgcggcgc
    66301 aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc
    66361 atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat accattccga
    66421 aacattattt cgacgaagcg ctaagtggcg gtaagcctgg cgatgatgat gtgattgaaa
    66481 tgatgtaaaa gacagattac ctggcctgat aagacgcgtg agcgtcgcat caggcatcgt
    66541 tcaccattgc cggatgcggc gttaacgcct tatccggcct acggttgagc actcagctta
    66601 cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc
    66661 acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttgtggtcga cgcgcttgag
    66721 atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata
    66781 tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcagg cgacgagctg
    66841 ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc
    66901 attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg
    66961 agcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt
    67021 atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg
    67081 ctgcccggaa agccaccagt ccaccgtata acgcagttga tgtttttcaa gcagggcaag
    67141 cacctgtgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac
    67201 aaacagttca cccggaataa cattgttact gcccgttccc gcctgaatat tagcaatctg
    67261 catactggtc gccgggaaaa attcattgcc ctgatcccac tcaatagcca ctaattcatt
    67321 aaggaaaggt gctgcgcgat gtactggatt gtcagccaga tgcgggtagg ccacatgccc
    67381 ctgaacgcca tgaatggtaa ggttgcaggt taacgatccg cgacgaccat ttttcaccac
    67441 atcaccaacc acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt
    67501 acgtgccatc aacgcttcga cgacttttac cgtaccgttg tgggcactgg cttcttcatc
    67561 agaggtgatc agaaatgcca gtcgccccgt atggttggga tgttgtgcga caaagcgttc
    67621 tgccgctacc accatcgccg ccagcgagcc tttcatatct gccgcaccgc gtccgaataa
    67681 catgccatca cgaatggtgg gttcaaacgg cggattgatc caacgatcgg cgtcgccagg
    67741 cggcaccacg tcggtatgcc cggcaaaggc taacgtttca ccttgcccac gccatgccca
    67801 aaaattctgc gtatcggcaa agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg
    67861 ttcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag
    67921 ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg
    67981 ataactggaa tcactgaaac ccagcagcat aggcttacca ggcgcgcaga gcaatggacg
    68041 tttgataatt gcaggcattt cagtcattaa tgccgccgca gaggccgcat cggtgatttt
    68101 attgcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttca gtaacgcttc
    68161 ccagcctaat tcgttgataa aaccgttcaa taattcgctg tccagcccat cgacgcggta
    68221 atcatgaaaa cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt
    68281 gtcacaattt ttgatgccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa
    68341 taaatactca tttaagggtg aaaacatttt gcctgcaaat gattatcagg aaatataaca
    68401 ttatcgaagt attcaaatta acaactatta aataatatta aacattttta acgtaaacat
    68461 taagaatatt catcttgtta tctgtctgta ttttaaccag aattaaatca gtattagatg
    68521 ccgtatgaat atctcgaaat gttgctgtgt atcacataca aattatcacc gcaggaccat
    68581 agtatttaca taaggtttgt cccccggagg tagtcatcgc agcaatcgag taaattagct
    68641 tcaccacggt atattgtttc cgtagaatac ccataattat aagagaggtt gttatgattg
    68701 aacgtgaact ggggaactgg aaagacttta tcgaagttat gcttcgtaag taattcccga
    68761 gacaaagtat aaacagcaag aacccgctga gcaggttctt aatcggaaag ttgaaaaagg
    68821 cgacacattg gcatgtcgcc ttttttattg cttattccgg gcgcggcttc agcgggaagc
    68881 ggcggcgcac cagcacaaag aacagcggca cgaagtaaat agccagaata gtagctgaaa
    68941 tcatcccgcc cattacgcca gtacccaccg catgctgacc accggaaccg gcacccgtgc
    69001 tggttgccat tggcaatacg ccgaagataa atgccagcga ggtcatcaga atcgggcgta
    69061 aacgctgacg gcaggcgtgg agcgtcgctt caaacaggtc gtggcctttt tggttcatct
    69121 cgttagcaaa ctcgacgatc aggatggcgt ttttcgccga taaaccaatg accgttaaca
    69181 ggcccacctg gaagtaaacg tcgttttcca gcccgcgcat ccaggttgcc agcagcgcgc
    69241 cgataacccc cagcggcacg accagcatta ccgagaacgg taccgaccag ctttcataca
    69301 atgccgccag acacaggaat actaccagca aggaaatggc gtacagcgcc ggagcctgcg
    69361 cgccggaaag ccgctcctga tacgacatcg ccgtccactc cagaccaaag ccgtttggca
    69421 actgcttcac taacgattcc ataatatcca tcgccgtacc ggtactgacc cccggtgcgg
    69481 cttccccaac aatctcaacc gcagaataac cgttatagcg ttccagacgc ggcgagcctg
    69541 tctcccagcg tgaggtcgcg aaagcagaga agggcaccat gccgccatct ttatttcgga
    69601 cataccagag attaatgtca tctggcagca tgcgatacgg cgcagctgcc tgcacataga
    69661 ctttcttcac gcgaccgcga tccataaagt cattcacata gctcgaaccc caggcggttt
    69721 gcagtgtgtc gttaatatcg tcgatagcaa cgcccagcgc ctgagcttta cgctggtcga
    69781 tatcaatctg caactgcgga ctgtcatcga ggccgttatg gcgcacacgg gttagctccg
    69841 ggttttccgc cgccagcgcc agcaactggt tacgtgctgc catcagcgca tcgtgacctg
    69901 ctccagcgtg gtcctgcaac tccatatcaa aacctgcaga actaccaaga ccgctaattg
    69961 ctggcgggct gctggcgata acgcgagctt ctttaatctt gttaaacgcc ttcgttgcac
    70021 gctcgataat ggcaaatgag gtgccggtct tactgtcgcg ttcgctccag tctttcaggc
    70081 ggataaacat tcgcgccacg ttttgcccgt tacccccggg gccagaacca acagtggcaa
    70141 acaccgacat gatgttgtct ttttcatggg tgaagtaata tttctcgatt tgctcaacga
    70201 ctttcagggt ctgttgttgg gttgaaccgc tgggcaattg taccgaggta gtaaacatgc
    70261 cacggtcttc cagcggtaag aacgacgtcg gcaaacgcag gaacaggaac accatgccgc
    70321 caagcagcag gacataaatc acaatccagc gcaggctacg gtggagaatt ttcgccaccc
    70381 ctttttcgta gcgttcggcg ttgcggttaa acatctggtt aaaccaggca aaaaagcctt
    70441 tttgcccgtg atgctcacct tttttcagtg gcttaagtag tgtggcacac agagccggag
    70501 tgaggatcat cgctaccagt actgacagca ccatcgccgc aacaatggta atagagaact
    70561 ggcgatagat ggcaccggtg gtgccgccga agaaggccat tggtacaaat accgccgaaa
    70621 gaaccatcgc aatcccgacc agtgccccct ggatctgccc catcgattta cgtgtggctt
    70681 cgcgaggagt gagtccttcc tcactcataa tacgttcgac gttttccacc accacgattg
    70741 cgtcatccac cagcagaccg atcgccagca ccatcgcgaa catggttaag gtgttgacgc
    70801 tgtaaccgaa ggcgtaaagt acggagaagg ttcccatcaa caccaccggc acggcgatag
    70861 tgggtatcag cgtggcgcgg aagttttgca ggaacagata cataacgagg aaaaccagag
    70921 cgatagcttc cagcagcgtt ttcaccacgt cttcaatgga ggctttaaca aacgaggtgg
    70981 tttcatacgc caccttgtat tccagtccgt gcgggaaata ctgcgccagc tcgtcgagac
    71041 gattgagcac cagctccgct gtcgccattt cgtttgcgcc ggaggccagt tttaccccca
    71101 gcccggaggc tggcttacca ttgaagcggc taagataatc gtatttctcc gcccccattt
    71161 cgacggtggc gacatcgccc agccttacct ctgagccgtc ttgattcacc ctcaaggtga
    71221 tatcgcggaa ctgttctggt gtttgcagca gtgactgggc gttaatggtg gcgttgagcg
    71281 cctgcttatc gacggaaggt gtaccaccaa gttgcccaac cgcaatctgc gcgttctgtg
    71341 actcaatggc atcagtgaca tctttagccg tcatctggaa actgttgagt ttcgccgggt
    71401 ccagccagat acgcatggaa tattgcgaac cataggcatc gatatccccg acgccattca
    71461 cgcggcttaa cgggtcctga atattactgg caacataatc ggcaatatcc tgtttatcca
    71521 tcgaaccatc ggtagagacg aaagcaatgg tcagaatatt ggtatcgccg gttttacgca
    71581 ccgtcacgcc ctgattttgc accgcctgcg gtaactttcg catggctgat tgcagctggt
    71641 tttgtacttg ctgcacggct tcgtccggat cggtgcctgc tttaaaactt aaagtgacag
    71701 atgcctgacc ggtgccactg ctctgagatg acatatacat gagattatcg aggccggtca
    71761 tattttgctc gataacctgg gtcacggtgt tttccagcgt ctgggccgat gcgccgggat
    71821 agttagcggt cactcgcaca ttcggtggcg cgagatcggg gtattgttca acgggcaatg
    71881 agaaaatcgc cagggtacct gtcagacaca acaggattgc cagcacccag gcaaaaatgg
    71941 ggcgatcaat aaagaaattc gccattaaaa gaggacctcg tgtttctgcg tatcgttgta
    72001 ggcttgactt gcttcactgt agcggcaagg tacgagccaa tcgtggagaa aaaaaggagt
    72061 taatgtaaat tatcatgccc ttttatactg aaccttaagt gcaagtattc tttggtcagt
    72121 aggaggcatt acatcagctg actttcctca ccctccgcgg agcgaaagct aatactcact
    72181 aacgtgccgc cgccggaagg ttgtgaaaaa gtcagcgtcc cacccagccg ttccgcgcgt
    72241 tcgcgcatga tattcagacc ataatgacct tcgggctctt tcggttcacc gatgccaatc
    72301 ccgttatcac ggatataaac cgtgtgattg ccgtccggcg cggtgacgca actgacggcg
    72361 atttcgctgg cgttggcgtg cttcatcgca ttcagcaccg cttcgcgaat aatttgcaac
    72421 aaatgcacct gcatttgcgc atccagcgcc agggttggca gacggcagtc gagggtcagt
    72481 ttggcgctgg tttgattttg taacgtatcc agcatttccc tcaacgcgga ggggagatcc
    72541 gcctgctgca gcgtcaggcg gaaagtagtc aacagctcgc gtaactgccg ataagcatca
    72601 ttcaacgcct gggaaaaatc ggccatgata ctttgtgcgg tggcgttatc ttccggtatc
    72661 gaacgcttca gtaacgtcaa ctggatacgt aagtaagaaa gtacctgagc cagcgagtcg
    72721 tgcaattcgc gggcgatggt tgcacgttct tccatcaaca ataactgctg aaaatgcttc
    72781 tgcgcctgat taaagtacaa accgcgtccc agcatcgacg aaacgctgtt aagcagcggt
    72841 tctgatgatg aaacgtgact attttgccag tgcagttcgc cgtaaaccgt ctcttgcatt
    72901 gtcaccggta aaatctgcat cggcaattcc gggtttggtt gcccttcgct aatccgccag
    72961 ttttcaccga catttaactc cagatattca gccgcttcat tgtcgcgaac aatctgcaaa
    73021 atatggcgga aacaatgcac atcaatctgg ctggtgttca gcgcctggga acattgatac
    73081 aacacctcca gacgacgctt ggcctcgtgg agatcgcggg tcttttcttc tactgacgcc
    73141 tccagcgaaa ggtacaattt atgcagctcg ctcgacatct ggttaaaggt ttttgcaagc
    73201 agaccaagct cattcggcag actggtatcc agcggcggcg agtcgaactg cccgtgttca
    73261 atacgttgac tggcggtaac cagctgattc agcggggcaa ccacctgatg gcgtatacgg
    73321 cgcaaagtaa aaaagaccag cgtgaaaata ccgataccgc cagccaggga aatcgccacc
    73381 accagcagca ttttgcgttc agcgtagtgc tgtaaagcca gcacgaacag gtctatctga
    73441 ttaacataat tattaatatt ggcctgatac cacggcaaat cgcccttgct gagccgatta
    73501 ttcatctcca gccagttggc attcagatgc gcatagcgag tttttactgc ttctggcaca
    73561 taccagacgt tgagattggt taataccggt gaatgcagtg cctgctgaaa caactggcga
    73621 tgtgcattga gttgtggact gccactttgc aagtcgtagc ccaggcggta actctgcata
    73681 cgtagcgatc cggcaatatt gatagcctca gcgtcgcgca aactgctcgc cagagttagc
    73741 agagcgatac ccgtggaaag aatcgacagc agcacaatgt aaaaaaaggc ccgggccaga
    73801 ctggccgaga cgggtcgttt aacaatcaca cgcgtcttct ccacaaaaaa tcattaatgt
    73861 tccagttagc tgaaacacaa tttagtcagt gatgggatta tttgatctgc cacagtttat
    73921 agcgaaatag ttacttattc tgggcacatg aacattgccg attcccctgt aagtcgggta
    73981 ataacaacac tcatataaag aataaggttt ttacaaccaa aaaagaaggt cgttatgaat
    74041 cgttttatta tggccaacag tcagcaatgt ctgggttgtc atgcttgtga aatcgcctgt
    74101 gtcatggctc acaatgatga gcaacatgtc ctgagccaac accattttca tccccgaatt
    74161 acggttatca aacatcaaca gcaacgtagt gcagtgacct gtcaccattg tgaagatgcg
    74221 ccctgcgccc gtagctgccc taatggcgca atcagccacg ttgatgacag cattcaggtc
    74281 aatcagcaaa agtgtattgg ctgtaaatcc tgcgtggtgg cctgtccttt tggtacgatg
    74341 caaatcgtcc tgacacccgt cgcggcagga aaagtaaaag ccacggcgca taaatgcgac
    74401 ctttgtgcgg ggcgcgaaaa cggtcctgcc tgtgttgaga attgcccggc ggacgcgctg
    74461 caactggtca ctgacgtcgc actctccggc atggcgaaat cccgccgctt gcgcaccgcg
    74521 cgtcaggaac atcaaccgtg gcatgccagt accgcggcgc aagaaatgcc ggtaatgagt
    74581 aaagtcgaac aaatgcaggc aacgcccgcg cgtggcgagc cggataaact ggcgattgaa
    74641 gcgcgcaaaa ccggttttga tgaaatttat ctgccatttc gcgccgacca ggcacaacgg
    74701 gaagcctcgc gctgccttaa gtgcggcgag cacagcgttt gtgaatggac ctgcccgctg
    74761 cataaccata ttccgcagtg gattgaactg gtgaaagccg gaaacatcga cgccgccgtc
    74821 gagctttctc accagaccaa caccctgccg gaaattaccg gacgcgtttg tccgcaagac
    74881 cgtttgtgtg aaggtgcctg tactattcgc gatgagcacg gtgctgtaac tatcggcaac
    74941 attgaacgct atatttcaga tcaggcgttg gcgaaaggtt ggcgtcctga cttaagtcat
    75001 gtcaccaaag tggacaagcg ggtggcgatt atcggggcag gcccggcagg gctggcctgt
    75061 gcggatgttc tgacccgcaa tggcgtggcg gtgacggtgt acgatcgcca tccagaaatt
    75121 ggtggcttgc tcactttcgg cattccttct ttcaaactgg ataaatccct gctggcacgc
    75181 cgtcgggaaa tcttcagcgc gatggggatt cacttcgaac tcaattgtga agtgggtaaa
    75241 gatgtctctt tggattcgct tttggaacaa tacgacgcgg tcttcgttgg cgtaggcact
    75301 taccgttcca tgaaagcggg tttacccaat gaagatgcgc cgggcgttta tgacgcgctg
    75361 ccgttcctca ttgccaacac taaacaggtg atggggctcg aagagctacc ggaagagccg
    75421 tttatcaata ccgccggact taacgtcgtg gtactgggcg gcggcgacac cgcgatggac
    75481 tgtgtgcgta ctgcgctgcg ccacggcgcg agtaacgtca cctgcgctta tcgtcgtgat
    75541 gaagctaaca tgccaggctc gaagaaagag gtgaagaacg cccgcgaaga gggggccaac
    75601 ttcgaattta acgtccagcc ggtggcgctt gagctgaatg aacaaggtca cgtttgcgga
    75661 attcgtttcc tgcgcacgcg tcttggcgag ccggatgccc aggggcgtcg gcgacctgta
    75721 ccgattgagg gcagtgaatt tgtcatgcca gccgatgcgg tgattatggc gtttggcttc
    75781 aatccacacg ggatgccgtg gctggagtcg cacggtgtaa cggtagacaa atggggccgc
    75841 atcatcgcgg atgtggaaag ccagtaccgt taccagacca ccaatccgaa aatcttcgcc
    75901 ggtggcgacg ccgtgcgtgg tgcggatctg gtggttaccg caatggcaga aggacgtcat
    75961 gcggcacagg ggattattga ctggctgggg gtaaaatcag tcaaatctca ctgatagcct
    76021 gcgcagacaa acccgacttc acagcgtaag ataattgttc atttcgcgct gtggagtcgg
    76081 tatgacgcaa caaatcaccc tcattaaaga caaaattctc tccgataact atttcaccct
    76141 gcacaacatt acctacgatc tcacgcgcaa agatggcgaa gttatccgcc ataaacgtga
    76201 agtttacgat cgcggtaatg gtgcgacgat cctcctgtac aacgcgaaga aaaagagcgt
    76261 ggttctgatt cgtcagttcc gtgtcgctac ctgggttaat ggcaatgaaa gcgggcagct
    76321 gattgaaacc tgcgccgggc tgctggataa cgacgaaccg gaagtatgca ttcgtaaaga
    76381 agcgattgaa gagacgggct atgaagtggg cgaagtgcgc aaattatttg aactgtatat
    76441 gtcgcctggc ggtgtgactg agctaatcca cttttttatc gccgaataca gtgacaatca
    76501 gcgcgctaac gccggtggcg gtgttgaaga tgaagatatt gaagtgctcg agctgccgtt
    76561 cagccaggcg ctggagatga tcaaaaccgg cgagatacgt gacggtaaga cggtgttatt
    76621 gcttaactat ttgcaaacgt cacatttaat ggactgaaaa ataacaataa tatttcgttg
    76681 tttattattg gctcaatccg acaaatctga ttgagccgcg ctacctgcgc aacgaagata
    76741 cgacttgtgc tgtttgtttg aacttctggg gtcgtaccgt ccatgcgcta tcgcattttc
    76801 cttctctttt ttttcgcttt gttgccgacg tctttggtgt gggcggcacc agcgcaacgg
    76861 gcgttttccg actggcaggt cacctgtaat aaccaaaact tctgcgtggc gcgcaatacg
    76921 ggcgatcata atggactggt gatgaccctg agccgcagcg ccggggcgca taccgatgcc
    76981 gttttacgta ttgagcgcgg cgggttgaag tcgccggatg cgtctgaagg agagatagcg
    77041 ccacggctgc tgttagatgg tgagccgttg gcgttaagtg gtgacaagtg gcggatttca
    77101 ccatggttat tagtaaccga tgatacggca accatcaccg cgtttttgca gatgattcag
    77161 gaagggaagg cgatcacctt acgcgacggc aatcagacca tttctctgag tggcttaaaa
    77221 gcggcgttgt tgtttattga tgctcagcaa aagcgcgttg gcagtgaaac cgcgtggatc
    77281 aagaaagggg acgaaccgcc gctcagcgta ccgcccgcgc ctgcgctgaa agaggtcgcg
    77341 gtggttaacc caacgccgac gccactctca ctcgaagaac gcaacgattt gctggattat
    77401 ggaaactggc ggatgaatgg tctgcgctgc tcgcttgatc cgttacgtcg tgaggtgaat
    77461 gtcactgcgc tgactgatga taaagcgctg atgatgatta gctgtgaggc aggggcctat
    77521 aacaccattg atttggcatg gattgtgtcg cgtaaaaagc cactagcctc gcgcccggtt
    77581 cggttgcgtt tgccattcaa caacggtcag gagacgaatg aactggaact gatgaacgca
    77641 acatttgatg agaaatcgcg tgaactggtg accttagcga aagggcgcgg attaagcgat
    77701 tgtggcattc aggcgcgctg gcgctttgac ggtcagcgtt ttcgcctggt gcgttatgcc
    77761 gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt gtggatcacc
    77821 cgttaaacaa aattgcgggg acatgtaggc cggataagac gctgtaggcc ggataagacg
    77881 cgtcagcgtc gcatccggca atcagcatcc ggcgatcacc atcaggcacc tttcactccc
    77941 agcaccttat gcgcttttgc cacaatattc tcggcggtaa agccaaagaa cgggaacagc
    78001 ttatccgccg gagcagattc cccgtaaccc gtcatcccga caattgcccc tttcagacca
    78061 acatacttgt accagtaatc ggcaatacct gcttccaccg ccacgcgagc cgcaacgtta
    78121 gaaggcaaca ccgactcccg atattcctca tcctgggcgt cgaaaatatc ggtcgagggc
    78181 agggaaacta cgcgtacatt gcgaccttct cccgctaatt tctctgccgc ttgcagggtg
    78241 atttccatct ctgaaccggt ggcaatcaga ataatatctg gcttaccgcc gctgtctttc
    78301 agcacataac cgccacgagc aatctcttta acctgatccg gcgtacgttc cacctgggcc
    78361 agattctgcc ttgagaggat cagtgctgtc ggcccgttgt ggcgctcaac cgccagcttc
    78421 cagcccaccg ccgcttccac ctgatcgcac ggtcgccagg tgctgaaatt cggcgttaag
    78481 cgcagactgg ccagttgctc aacggcctgg tgcgtcggac catcttcccc taagccaata
    78541 gaatcgtggg tataaaccat aatctgccgt gctttcatca gtgccgccat ccgcgcggca
    78601 ttacgggcgt attcaacaaa catcaggaac gtcgcggtat acggcacaaa gccgccgtgg
    78661 tgcgcaatgc cgttggcgat agcggtcatg ccaaattcac gcaccccgta gtgaatgtag
    78721 ttgcccgccg gatcttcctt cagcgacaca gagcctttcc agatggtgag gttgctggga
    78781 gccagatccg ctgaacctcc gagcagctcc ggtagcatcg gtccgtaagc gttaagcgta
    78841 ttttgcgaag ccttacgggt ggcgattttc gccggattcg cctgcaactc attgatatat
    78901 ttctgagtcg ttttctccca gtccttcggt aaaccaccgc tcatccgacg ggtaaactct
    78961 tctgccagtt gcggatgagc ctttttatag gcggcaaact tctcattcca gctctgctgc
    79021 gctttttcgc ctttttcgcg ggcatcccag gcgtgataaa tctctttagg gatctcaaat
    79081 ggcggatggt gccagcccag tttttgccgt gccagcgcca cttcttcttc ccccagaggt
    79141 gcgccgtgcg cctcttcctt acctgcttta ttcggcgaac caaagccaat caccgtacgg
    79201 cagataatca gcgacggctt atctttcacg ctttgcgctt caaggatcgc ttccttcacc
    79261 gcctgcggat cgtgaccgtc gatttcatgg atcacatgcc agtgataggc ttcaaaacgt
    79321 tttgccgtat cgtcggtaaa ccagccttct gtttcaccgt cgatggaaat accgttgtga
    79381 tcgtaaaaac caatcagctt gcccagtccc agcgtgcccg ccagcgaaca gacttcgtgg
    79441 gaaatacctt ccatcaggca gccgtcgccc ataaacacat aggtgaagtg atcgacaatt
    79501 tcatgatccg gctgattaaa ctgcgccgcc agtgtacgct ctgctatcgc cagtccgacg
    79561 gcgttcgcca aaccttgtcc aagcggaccg gtggtggttt caacacctgg cgtatagcca
    79621 atctccgggt ggcctggggt tttcgaatgc aactgacgga agttcttcag ttcttccagc
    79681 ggcaggtcgt aaccggtcag atgtagcaaa ctgtagagca gcatcgacgc gtgaccgttg
    79741 gaaagaataa agcggtcgcg atcataccag gttgggtcgg taggattatg tttaagaaaa
    79801 tcgttccaca gcacttcggc aatatcagcc atgcccatcg gcgcgccggg atgaccagaa
    79861 ttggcttttt gtaccgcatc catactgagt gcgcgaatcg cattggcaag gtcttttcgg
    79921 gacatataac actccgtggc tggtttatag tttggcggca agaagatctt ccagtttgcg
    79981 ttgatcaaca gcgaacagac gaatgccttc agacagtttt tctaccgcca tcgcatcctg
    80041 attgtgctcc caacggaact ccgcttcgct catgggagct gggcgtggga acgtctgaga
    80101 aggtgggatt aatttacgta ccactggcga aactttttcc tgcagctcct tcagcaaatt
    80161 cggtgcgata gtcagtcgat cgcagccggt taaggcgagg atttgttcgg tgcgacggaa
    80221 gctcgcgccc atcacaatgg tttcatagtg gtgttgctta tagtagtcgt agatattgcg
    80281 caccgattta acgcccggat cttcttccac cacatacggg tccatcggct tgcgtgcctg
    80341 ataccagtca taaatacgcc cgacaaacgg cgaaatcaga aaaacgcccg cttctgcaca
    80401 ggcccgcgcc tgggcaaaag aaaacagcag cgtcaggttg cagttaatac cttctttttc
    80461 cagctcttcg gccgcgcgaa ttccttccca ggtcgaagcc agtttgatca aaatgcgtga
    80521 tttctcaacg ccttgttgct gatacaagtc caccagatgg cgcgccttct caatactctt
    80581 ttctttatca aaagagaggc gtgcatcaac ttctgttgac acgcgaccgg gtacgatttt
    80641 gagaatttca gcaccgaaat tgactgccag tttgtcacac gctgcgacca cctgttgttc
    80701 ctgggtcttg ccattttttt taccccaggc gatagcatcg tctattaaat gctcatattg
    80761 tgataatccg gcagctttga gtaacagcga aggattggtg gtggcatcct ggggatgata
    80821 atggcgaatg gactcaatat cgccgctgtc tgccacgaca gtggtgaact gtttgatgcc
    80881 gtctaactcg ttcataggaa atactccttg aaaagtaaag tgttagatga gtgcgttaat
    80941 tcacacttct gagaaatttc gctaaacgca tcaaaaaagc atagcagaca ggcatggtat
    81001 tgctggatta agcaggtaac atcagtgtta taggattatt accaaaacat tatattaatg
    81061 gtgagagttt ggacttgctc aaagtctgta gactccggca gggtaataat gtgcgccacg
    81121 ttgtgggcag gggctgttgc ccacacactt tatttgtgaa cgttacgtga aaggaacaac
    81181 caaatggatg accagttaaa acaaagtgca cttgatttcc atgaatttcc agttccaggg
    81241 aaaatccagg tttctccaac caagcctctg gcaacgcagc gcgatctggc gctggcctac
    81301 tcaccaggcg ttgccgcacc ttgtcttgaa atcgaaaaag acccgttaaa agcctacaaa
    81361 tataccgcgc gcggtaacct ggtggcggtg atctctaacg gtacggcggt gctggggtta
    81421 ggcaacattg gcgcgctggc tggcaaaccg gtgatggaag gcaagggcgt tctgtttaag
    81481 aaattcgccg ggattgatgt atttgacatt gaagttgatg a
//
LOCUS       DAHBHC010000009        81516 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHBHC010000009 DAHBHC010000000
VERSION     DAHBHC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.009"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..397)
                     /gene="aceF"
                     /locus_tag="M2A35_000820"
     CDS             complement(<1..397)
                     /gene="aceF"
                     /locus_tag="M2A35_000820"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="E2 component of pyruvate dehydrogenase multienzyme
                     complex; in Escherichia coli AceF contains three
                     N-terminal lipoyl domains; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCB7197155.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVE"
     gene            complement(412..3075)
                     /gene="aceE"
                     /locus_tag="M2A35_000821"
     CDS             complement(412..3075)
                     /gene="aceE"
                     /locus_tag="M2A35_000821"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCB7197156.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(3236..4000)
                     /gene="pdhR"
                     /locus_tag="M2A35_000822"
     CDS             complement(3236..4000)
                     /gene="pdhR"
                     /locus_tag="M2A35_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCB7197157.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            4541..5914
                     /gene="aroP"
                     /locus_tag="M2A35_000823"
     CDS             4541..5914
                     /gene="aroP"
                     /locus_tag="M2A35_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCB7197158.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(5957..6811)
                     /gene="ampE"
                     /locus_tag="M2A35_000824"
     CDS             complement(5957..6811)
                     /gene="ampE"
                     /locus_tag="M2A35_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCB7197159.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTCGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPYVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(6808..7359)
                     /gene="ampD"
                     /locus_tag="M2A35_000825"
     CDS             complement(6808..7359)
                     /gene="ampD"
                     /locus_tag="M2A35_000825"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCB7197160.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            7447..8340
                     /gene="nadC"
                     /locus_tag="M2A35_000826"
     CDS             7447..8340
                     /gene="nadC"
                     /locus_tag="M2A35_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCB7197161.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            8543..8983
                     /gene="ppdD"
                     /locus_tag="M2A35_000827"
     CDS             8543..8983
                     /gene="ppdD"
                     /locus_tag="M2A35_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCB7197162.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            8993..10378
                     /gene="gspE"
                     /locus_tag="M2A35_000828"
     CDS             8993..10378
                     /gene="gspE"
                     /locus_tag="M2A35_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCB7197163.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            10368..11570
                     /gene="hofC"
                     /locus_tag="M2A35_000829"
     CDS             10368..11570
                     /gene="hofC"
                     /locus_tag="M2A35_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCB7197164.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(11605..12648)
                     /gene="guaC"
                     /locus_tag="M2A35_000830"
     CDS             complement(11605..12648)
                     /gene="guaC"
                     /locus_tag="M2A35_000830"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCB7197165.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(12804..12848)
                     /locus_tag="M2A35_000831"
     CDS             complement(12804..12848)
                     /locus_tag="M2A35_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197166.1"
                     /translation="MIIKTLKMSARKRN"
     gene            12873..13493
                     /gene="coaE"
                     /locus_tag="M2A35_000832"
     CDS             12873..13493
                     /gene="coaE"
                     /locus_tag="M2A35_000832"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCB7197167.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            13493..14236
                     /gene="zapD"
                     /locus_tag="M2A35_000833"
     CDS             13493..14236
                     /gene="zapD"
                     /locus_tag="M2A35_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCB7197168.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            14246..14443
                     /gene="yacG"
                     /locus_tag="M2A35_000834"
     CDS             14246..14443
                     /gene="yacG"
                     /locus_tag="M2A35_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCB7197169.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(14543..14932)
                     /gene="mutT"
                     /locus_tag="M2A35_000835"
     CDS             complement(14543..14932)
                     /gene="mutT"
                     /locus_tag="M2A35_000835"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCB7197170.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGAPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(14992..17697)
                     /gene="secA"
                     /locus_tag="M2A35_000836"
     CDS             complement(14992..17697)
                     /gene="secA"
                     /locus_tag="M2A35_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCB7197171.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(17759..18271)
                     /gene="secM"
                     /locus_tag="M2A35_000837"
     CDS             complement(17759..18271)
                     /gene="secM"
                     /locus_tag="M2A35_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCB7197172.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(18502..19419)
                     /gene="lpxC"
                     /locus_tag="M2A35_000838"
     CDS             complement(18502..19419)
                     /gene="lpxC"
                     /locus_tag="M2A35_000838"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCB7197173.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(19520..20671)
                     /gene="ftsZ"
                     /locus_tag="M2A35_000839"
     CDS             complement(19520..20671)
                     /gene="ftsZ"
                     /locus_tag="M2A35_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCB7197174.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(20732..21994)
                     /gene="ftsA"
                     /locus_tag="M2A35_000840"
     CDS             complement(20732..21994)
                     /gene="ftsA"
                     /locus_tag="M2A35_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCB7197175.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(21991..22821)
                     /gene="ftsQ"
                     /locus_tag="M2A35_000841"
     CDS             complement(21991..22821)
                     /gene="ftsQ"
                     /locus_tag="M2A35_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCB7197176.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGPMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(22823..23743)
                     /gene="ddlB"
                     /locus_tag="M2A35_000842"
     CDS             complement(22823..23743)
                     /gene="ddlB"
                     /locus_tag="M2A35_000842"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCB7197177.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(23736..25211)
                     /gene="murC"
                     /locus_tag="M2A35_000843"
     CDS             complement(23736..25211)
                     /gene="murC"
                     /locus_tag="M2A35_000843"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCB7197178.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(25265..26332)
                     /gene="murG"
                     /locus_tag="M2A35_000844"
     CDS             complement(25265..26332)
                     /gene="murG"
                     /locus_tag="M2A35_000844"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCB7197179.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(26329..27573)
                     /gene="ftsW"
                     /locus_tag="M2A35_000845"
     CDS             complement(26329..27573)
                     /gene="ftsW"
                     /locus_tag="M2A35_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCB7197180.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(27573..28889)
                     /gene="murD"
                     /locus_tag="M2A35_000846"
     CDS             complement(27573..28889)
                     /gene="murD"
                     /locus_tag="M2A35_000846"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCB7197181.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(28892..29974)
                     /gene="mraY"
                     /locus_tag="M2A35_000847"
     CDS             complement(28892..29974)
                     /gene="mraY"
                     /locus_tag="M2A35_000847"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCB7197182.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(29968..31326)
                     /gene="murF"
                     /locus_tag="M2A35_000848"
     CDS             complement(29968..31326)
                     /gene="murF"
                     /locus_tag="M2A35_000848"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCB7197183.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(31323..32810)
                     /gene="murE"
                     /locus_tag="M2A35_000849"
     CDS             complement(31323..32810)
                     /gene="murE"
                     /locus_tag="M2A35_000849"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCB7197184.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDV
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(32797..34563)
                     /gene="ftsI"
                     /locus_tag="M2A35_000850"
     CDS             complement(32797..34563)
                     /gene="ftsI"
                     /locus_tag="M2A35_000850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCB7197185.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(34579..34944)
                     /gene="ftsL"
                     /locus_tag="M2A35_000851"
     CDS             complement(34579..34944)
                     /gene="ftsL"
                     /locus_tag="M2A35_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCB7197186.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(34941..35882)
                     /gene="rsmH"
                     /locus_tag="M2A35_000852"
     CDS             complement(34941..35882)
                     /gene="rsmH"
                     /locus_tag="M2A35_000852"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCB7197187.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(35884..36342)
                     /gene="mraZ"
                     /locus_tag="M2A35_000853"
     CDS             complement(35884..36342)
                     /gene="mraZ"
                     /locus_tag="M2A35_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCB7197188.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(36944..37948)
                     /gene="cra"
                     /locus_tag="M2A35_000854"
     CDS             complement(36944..37948)
                     /gene="cra"
                     /locus_tag="M2A35_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCB7197189.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(38030..38116)
                     /locus_tag="M2A35_000855"
     CDS             complement(38030..38116)
                     /locus_tag="M2A35_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCB7197190.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(38128..38619)
                     /gene="ilvN"
                     /locus_tag="M2A35_000856"
     CDS             complement(38128..38619)
                     /gene="ilvN"
                     /locus_tag="M2A35_000856"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCB7197191.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(38622..40346)
                     /gene="ilvI"
                     /locus_tag="M2A35_000857"
     CDS             complement(38622..40346)
                     /gene="ilvI"
                     /locus_tag="M2A35_000857"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCB7197192.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            40465..40509
                     /locus_tag="M2A35_000858"
     CDS             40465..40509
                     /locus_tag="M2A35_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197193.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(40664..41608)
                     /gene="leuO"
                     /locus_tag="M2A35_000859"
     CDS             complement(40664..41608)
                     /gene="leuO"
                     /locus_tag="M2A35_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCB7197194.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            42281..42367
                     /gene="leuL"
                     /locus_tag="M2A35_000860"
     CDS             42281..42367
                     /gene="leuL"
                     /locus_tag="M2A35_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCB7197195.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            42460..44031
                     /gene="leuA"
                     /locus_tag="M2A35_000861"
     CDS             42460..44031
                     /gene="leuA"
                     /locus_tag="M2A35_000861"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCB7197196.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            44031..45122
                     /gene="leuB"
                     /locus_tag="M2A35_000862"
     CDS             44031..45122
                     /gene="leuB"
                     /locus_tag="M2A35_000862"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCB7197197.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            45125..46525
                     /gene="leuC"
                     /locus_tag="M2A35_000863"
     CDS             45125..46525
                     /gene="leuC"
                     /locus_tag="M2A35_000863"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCB7197198.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            46536..47141
                     /gene="leuD"
                     /locus_tag="M2A35_000864"
     CDS             46536..47141
                     /gene="leuD"
                     /locus_tag="M2A35_000864"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCB7197199.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(47190..48368)
                     /gene="setA"
                     /locus_tag="M2A35_000865"
     CDS             complement(47190..48368)
                     /gene="setA"
                     /locus_tag="M2A35_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCB7197200.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(48470..48601)
                     /gene="sgrT"
                     /locus_tag="M2A35_000866"
     CDS             complement(48470..48601)
                     /gene="sgrT"
                     /locus_tag="M2A35_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCB7197201.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            48690..50345
                     /gene="sgrR"
                     /locus_tag="M2A35_000867"
     CDS             48690..50345
                     /gene="sgrR"
                     /locus_tag="M2A35_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCB7197202.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      50379..50478
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            50509..51492
                     /gene="thiB"
                     /locus_tag="M2A35_000868"
     CDS             50509..51492
                     /gene="thiB"
                     /locus_tag="M2A35_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCB7197203.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            51468..53078
                     /gene="thiP"
                     /locus_tag="M2A35_000869"
     CDS             51468..53078
                     /gene="thiP"
                     /locus_tag="M2A35_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCB7197204.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            53062..53760
                     /gene="thiQ"
                     /locus_tag="M2A35_000870"
     CDS             53062..53760
                     /gene="thiQ"
                     /locus_tag="M2A35_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCB7197205.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(53874..54638)
                     /locus_tag="M2A35_000871"
     CDS             complement(53874..54638)
                     /locus_tag="M2A35_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7197206.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(54724..55602)
                     /gene="araC"
                     /locus_tag="M2A35_000872"
     CDS             complement(54724..55602)
                     /gene="araC"
                     /locus_tag="M2A35_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCB7197207.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            55941..57641
                     /gene="araB"
                     /locus_tag="M2A35_000873"
     CDS             55941..57641
                     /gene="araB"
                     /locus_tag="M2A35_000873"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCB7197208.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPLLNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            57652..59154
                     /gene="araA"
                     /locus_tag="M2A35_000874"
     CDS             57652..59154
                     /gene="araA"
                     /locus_tag="M2A35_000874"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCB7197209.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHGVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            59354..60049
                     /gene="araD"
                     /locus_tag="M2A35_000875"
     CDS             59354..60049
                     /gene="araD"
                     /locus_tag="M2A35_000875"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCB7197210.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            60124..62475
                     /gene="polB"
                     /locus_tag="M2A35_000876"
     CDS             60124..62475
                     /gene="polB"
                     /locus_tag="M2A35_000876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCB7197211.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            62640..65546
                     /gene="rapA"
                     /locus_tag="M2A35_000877"
     CDS             62640..65546
                     /gene="rapA"
                     /locus_tag="M2A35_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCB7197212.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            65558..66217
                     /gene="rluA"
                     /locus_tag="M2A35_000878"
     CDS             65558..66217
                     /gene="rluA"
                     /locus_tag="M2A35_000878"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCB7197213.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(66334..67149)
                     /gene="djlA"
                     /locus_tag="M2A35_000879"
     CDS             complement(66334..67149)
                     /gene="djlA"
                     /locus_tag="M2A35_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCB7197214.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            67404..69758
                     /gene="lptD"
                     /locus_tag="M2A35_000880"
     CDS             67404..69758
                     /gene="lptD"
                     /locus_tag="M2A35_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCB7197215.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            69811..71097
                     /gene="surA"
                     /locus_tag="M2A35_000881"
     CDS             69811..71097
                     /gene="surA"
                     /locus_tag="M2A35_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCB7197216.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            71097..72086
                     /gene="pdxA"
                     /locus_tag="M2A35_000882"
     CDS             71097..72086
                     /gene="pdxA"
                     /locus_tag="M2A35_000882"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCB7197217.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            72083..72904
                     /gene="rsmA"
                     /locus_tag="M2A35_000883"
     CDS             72083..72904
                     /gene="rsmA"
                     /locus_tag="M2A35_000883"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCB7197218.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            72907..73284
                     /gene="apaG"
                     /locus_tag="M2A35_000884"
     CDS             72907..73284
                     /gene="apaG"
                     /locus_tag="M2A35_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCB7197219.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            73291..74133
                     /gene="apaH"
                     /locus_tag="M2A35_000885"
     CDS             73291..74133
                     /gene="apaH"
                     /locus_tag="M2A35_000885"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCB7197220.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(74211..74690)
                     /gene="folA"
                     /locus_tag="M2A35_000886"
     CDS             complement(74211..74690)
                     /gene="folA"
                     /locus_tag="M2A35_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCB7197221.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(74882..76744)
                     /gene="kefC"
                     /locus_tag="M2A35_000887"
     CDS             complement(74882..76744)
                     /gene="kefC"
                     /locus_tag="M2A35_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCB7197222.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGFIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LLCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(76737..77267)
                     /gene="kefF"
                     /locus_tag="M2A35_000888"
     CDS             complement(76737..77267)
                     /gene="kefF"
                     /locus_tag="M2A35_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCB7197223.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(77375..78706)
                     /locus_tag="M2A35_000889"
     CDS             complement(77375..78706)
                     /locus_tag="M2A35_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7197224.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(78765..79052)
                     /gene="fixX"
                     /locus_tag="M2A35_000890"
     CDS             complement(78765..79052)
                     /gene="fixX"
                     /locus_tag="M2A35_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCB7197225.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWKYPRGTFGVEFRYG"
     gene            complement(79049..80335)
                     /locus_tag="M2A35_000891"
     CDS             complement(79049..80335)
                     /locus_tag="M2A35_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCB7197226.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(80386..81327)
                     /locus_tag="M2A35_000892"
     CDS             complement(80386..81327)
                     /locus_tag="M2A35_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCB7197227.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(81342..>81516)
                     /locus_tag="M2A35_000893"
     CDS             complement(81342..>81516)
                     /locus_tag="M2A35_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="required for anaerobic carnitine reduction, may act
                     to transfer electrons to crotonobetaine reductase; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCB7197228.1"
                     /translation="KKPVQVWSAADIGFNAEAAWSEQQVAAPKQRERQRIVIEGDGEE
                     QIAAFAENLRKVI"
BASE COUNT        19558 a        22531 c        20688 g        18739 t
ORIGIN      
        1 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
       61 tatccggaac gttaacgtct tttgccgctg cagccgctgg tgctgctgcc ggagctgttt
      121 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
      181 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
      241 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
      301 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
      361 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
      421 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
      481 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gctacaacca cgtaggaagc
      541 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
      601 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
      661 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
      721 gcgcggagtt tccagcgggt gcagcatgtt ccagcgttca caatcctgac catctcgcgc
      781 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
      841 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
      901 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
      961 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
     1021 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
     1081 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
     1141 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
     1201 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
     1261 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
     1321 agaggtcgcc gctgccagcc aggaacaacc tgcgcccaac tcgttgatcc cttcctgcag
     1381 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
     1441 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
     1501 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
     1561 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
     1621 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
     1681 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
     1741 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
     1801 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
     1861 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
     1921 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
     1981 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
     2041 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
     2101 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
     2161 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
     2221 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
     2281 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
     2341 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
     2401 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
     2461 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
     2521 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
     2581 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
     2641 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
     2701 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
     2761 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
     2821 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
     2881 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
     2941 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
     3001 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
     3061 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
     3121 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
     3181 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
     3241 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
     3301 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
     3361 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
     3421 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
     3481 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
     3541 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
     3601 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
     3661 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
     3721 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
     3781 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
     3841 cctcacgcaa ggagggacga gagacgtcaa actgttttgc cagttcgcgt tccggtggga
     3901 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
     3961 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
     4021 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
     4081 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
     4141 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
     4201 agttttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
     4261 tgtgattttt aattcaatta aaacgaattt aaattcattc tacatattga gaggggttga
     4321 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg
     4381 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc
     4441 aaacttcttt gatgtaaaca aattaataca acaaacggaa ttgcaaactt acacacgcat
     4501 cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca
     4561 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
     4621 cgcgataggg acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg
     4681 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
     4741 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
     4801 gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc
     4861 catggctgag ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatccccac
     4921 ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa
     4981 agtgtttggc gagatggagt tctggtttgc cattatcaaa gttatcgcgg tggtagcgat
     5041 gatcatcttc ggcggctggc tgctattcag tggcaacggc ggcccgcagg cgaccgttag
     5101 caacctgtgg gatcagggtg gtttcctgcc gcacggcttc accgggctgg tgatgatgat
     5161 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
     5221 tgataacccg gagcaaagta taccgaaagc aactaaccag gttatctacc gcatcctgat
     5281 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
     5341 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
     5401 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
     5461 cagccgtatg ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt
     5521 cgataaacgt ggtgtaccag taaataccat tctggtgtct gcactggtaa cggcgttgtg
     5581 cgtactgatt aactaccttg ccccagagtc cgctttcgga ctgttaatgg cgctggtggt
     5641 atctgcactg gtaatcaact gggcgatgat tagcctggcg catatgaaat tccgtcgcgc
     5701 caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg
     5761 gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc
     5821 gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa
     5881 agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca cccgtacagg
     5941 gtgagggcaa taatctttac accaacgccc cgtaaatcgt cagtagtgca atcaccacca
     6001 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat
     6061 cgacatacgg ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg
     6121 aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
     6181 tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg
     6241 catcaatgcc ggactgtaaa cgatgatgcg gcgtctgata tcgcgccagc cagtattgcc
     6301 atgcacgcaa aaacgcatac cccatcagcg taacgggtcc acaggttccc cccacaatca
     6361 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
     6421 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
     6481 cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
     6541 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagta
     6601 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca
     6661 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
     6721 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
     6781 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcacacgaaa
     6841 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
     6901 gcccgtcatg tttttagcga tatccggata gcaatcaatc agtgcccgcg taaccgccgc
     6961 aagctgttga tactgcgcat cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc
     7021 aatagaaaaa tcattgcagc gttcgcgccc ctgatactga gagactcccg catgccatgc
     7081 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
     7141 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
     7201 agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
     7261 caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac
     7321 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
     7381 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
     7441 tttaacatgc cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc
     7501 attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca
     7561 gtcgatgcca acaatgatat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
     7621 acggtgatca cccgcgagaa tggcgtcttt tgcggcaaac gctgggttga agaggtgttt
     7681 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
     7741 aatgccaatc aatccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
     7801 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
     7861 gtcgaattgc tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacccggc
     7921 ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg
     7981 ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc agtgcgccag
     8041 gcggtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat
     8101 ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc
     8161 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
     8221 tctggcaacg tcactgacaa aacactgcgt gaatttgccg aaacgggcgt ggactttatc
     8281 tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa
     8341 tagttgacag atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt
     8401 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg
     8461 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcgcca
     8521 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
     8581 ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta
     8641 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
     8701 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
     8761 tccctcgcct accaccaccc actatgtttc agccatgagt gtggcaaaag gcgtggtgtc
     8821 gctgaccggg caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa
     8881 cgcaaacggc gtcaccggct ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca
     8941 tcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
     9001 tccacagctc actgccctgt gtctgcgtta tcatggagtc ttgctggatg ccagcgaaga
     9061 ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ctactggacg cattgcattt
     9121 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
     9181 cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga
     9241 gttgctaact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
     9301 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
     9361 ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct
     9421 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
     9481 cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
     9541 gttgttacag caggtgagcc aggcactgga tgtcaacacg cttggaatgc agccgttaca
     9601 actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
     9661 tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaacgctga ataccgctga
     9721 cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac
     9781 gcaaatccat ccgcgtgccg gactcacctt tcagggcgtg ttgcgtgcgt tattgcgcca
     9841 ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacagcag agatcgctat
     9901 taaagcggcg caaactggtc acctggtgtt gtctacccta cacactaatt ccacctgcga
     9961 aacgctggta cgtttacagc aaatgggggt cgcccgctgg atgctatcat cggcgcttac
    10021 gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc ggcagcaagg
    10081 ggagcccatc cacattccag acaatgtatg gccatcgccg ctgccccact ggcaggcacc
    10141 cggttgtgta cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc
    10201 cataacgccg gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac
    10261 gcacgcacga caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca
    10321 aggcttaacc acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc
    10381 aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg
    10441 cagagagccg tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc
    10501 tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc
    10561 atcaactggc gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg
    10621 cggaacagca tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg
    10681 aacagggcat tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct
    10741 atcaggcaat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac
    10801 tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt
    10861 atcccatcat cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc
    10921 tgccggagtt tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg
    10981 ggatcatgac gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct
    11041 ttctgctggc gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc
    11101 agaaattgct gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc
    11161 agatttttac gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag
    11221 agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc
    11281 acgatatcag taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc
    11341 cgctctgttt gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag
    11401 acaacctcgc ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct
    11461 tactggaacc ggcgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga
    11521 tgtatctgcc aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg
    11581 ttgctccaca ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg
    11641 cacacggata aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt
    11701 acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag
    11761 cggcagctta acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg
    11821 tttcatcgca gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc
    11881 aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc
    11941 ggcaccgccg ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac
    12001 gatcattccg cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg
    12061 ataaccgacg cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt
    12121 aacgatatca gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc
    12181 agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg
    12241 ttcggaataa ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag
    12301 aatctgttta gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt
    12361 cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt
    12421 atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac
    12481 ggtgtccata tttgcggcga taattggcac gccggaccag ctctgacctg aatgtttgaa
    12541 ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg
    12601 gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct
    12661 ggggttaatg gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc
    12721 aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg
    12781 gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg
    12841 ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag
    12901 gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca
    12961 ttgatgccga tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca
    13021 ttgctgatca ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct
    13081 tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc
    13141 cgctgattca gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt
    13201 gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg
    13261 tggatgtcag cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg
    13321 agcatgtcga acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag
    13381 atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc
    13441 acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac
    13501 ccaggtcctt tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt
    13561 tttgattcag caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt
    13621 cttccgtaat gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct
    13681 gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt
    13741 ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat
    13801 ttccgcgccg cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca
    13861 gcgactgagc atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct
    13921 gcatctaccc caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc
    13981 gctcacccag gcacttacca tggtgctgga tttaattcgc cagtcggctc ccttccgtaa
    14041 acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct
    14101 gaatctgtcg ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc
    14161 cattcgtttt atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga
    14221 actggcctgt tgctaaggag tgaaaatgtc agaaactatt acggtgaatt gcccaacctg
    14281 cgggaaaacg gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg
    14341 tcagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga
    14401 tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc
    14461 acgtaggcct gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg
    14521 cggcgtgaac gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg
    14581 ctggcggaaa atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt
    14641 ctttacccca cggcgcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc
    14701 tgtccgggaa ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga
    14761 cttcttcctg aagttcacgc accaccgcct gttccggcgt ttcgcccatt tcaattttac
    14821 cgccgggaaa ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga
    14881 tttcattgtt ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa
    14941 acctataaaa aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag
    15001 gcgaccatgg cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc
    15061 tactttgcgc tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg
    15121 atggctaagc tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag
    15181 ctcctcaacc tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata
    15241 ttttaacgac tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt
    15301 cggatctttc tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgctgc
    15361 caggtgctct ttccacaggg agtcaagcgt ttgcagcatg acgcctttct cgaagtgacg
    15421 catcatctca gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag
    15481 aatgcgctca cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat
    15541 tggtaaatcg aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat
    15601 ttcttccagc gactgtggcg gaatgtaggc atcaatggtg gctttgaaca catcttcacg
    15661 aatgctgttg atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta
    15721 aatggcgcga cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc
    15781 gaagttacga ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg
    15841 ttcaatggct tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga
    15901 agcaaaaata cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc
    15961 cccctgacga ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc
    16021 ggtaccgatg atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc
    16081 ggctttaatt ttttcaattt gctctacggt cggattttcc agcgcggcaa cttctgcctg
    16141 ccagctacca ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac
    16201 cgcagccgga taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt
    16261 caggacgttg tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc
    16321 gatggagata gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc
    16381 aatgatcgcc tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg
    16441 aatcattgga cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc
    16501 gaaagcttcg gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta
    16561 gttctggaag gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt
    16621 cgcttccaca gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt
    16681 gtgttcgtca acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa
    16741 cagcgcatga gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga
    16801 gtacagagac tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac
    16861 cagaccacgt tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc
    16921 gccctggaag gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt
    16981 cacgcgttta tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg
    17041 cgcttcatcg atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg
    17101 tacacgttct tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt
    17161 gttcgtaccg taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc
    17221 cggcaggttg ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc
    17281 gtcacgttgc gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc
    17341 gttcaggtaa gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc
    17401 gatgcagcgt tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc
    17461 aaagacgcgc ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag
    17521 cacttcgcct ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc
    17581 ggagagtttt tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg
    17641 gcgcagggtg cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat
    17701 aaaatctcaa acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt
    17761 aggtgaggcg ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc
    17821 tgaattttgc ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg
    17881 tgccttcctg ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa
    17941 gaggcaaaga ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac
    18001 ggattaccgt gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc
    18061 ggcgtgtgtt cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt
    18121 ggttgcgggt tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca
    18181 aacctaaact cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa
    18241 actgtcgcca gcgcgtcagt attccactca cattattgcc atcccgatcg tattcaaacg
    18301 tatttagcgc acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt
    18361 tgtgcggaaa agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat
    18421 ttaaaaaaaa acgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt
    18481 tatacgacag tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg
    18541 cagttctgcg tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc
    18601 ctgcagcagt ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat
    18661 attgtgacca cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc
    18721 gtcttcaaaa cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca
    18781 atcgaagctg ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa
    18841 accgaacgta cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc
    18901 atagcgctgg ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga
    18961 aaaaccattg tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc
    19021 tttgatgcga acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag
    19081 gtatacaaac ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat
    19141 aacaatgtta tcgatgccca agcccgcgag agcagcattg aggtgctcta cggttgaaat
    19201 ccgtacatca tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc
    19261 atcggccggg aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt
    19321 ggccggcgca gggcgtaacg tcagggtgac tttcttgccg gtatgtaaac cgacacccgt
    19381 cgcctgaacg atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac
    19441 ctatccaact gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa
    19501 cccaaattcc agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat
    19561 aatccggctc tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct
    19621 gctcctgggt cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa
    19681 cctgcttatt ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa
    19741 caacggttac gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag
    19801 tcgcgttgtc ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac
    19861 gcaggtcgaa gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat
    19921 cttccagcag cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc
    19981 tcgccacgcc agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg
    20041 caaagtccac gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc
    20101 ctttcagtac atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa
    20161 ctttcagcag tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag
    20221 tgatcccctg ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga
    20281 cgacagcaac ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac
    20341 ctgtaccggt accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg
    20401 ccgcacgcaa tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc
    20461 cagcgcccag tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt
    20521 tacgcagcgc ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atgcgctcgc
    20581 gcaccatgtg ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg
    20641 cgtcattggt aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct
    20701 gaggccgtaa tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga
    20761 gtcgcttgat ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt
    20821 taagatgtga ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct
    20881 gagcataatc cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa
    20941 acacgcgctg agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa
    21001 tgcctgccgc caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata
    21061 tctcttcgtt gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg
    21121 tctgacgttg cagactccgc ggcggacgac cacctacgct cggcacttcc acgctctcat
    21181 cttttccaac gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc
    21241 ttggcggcgt gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa
    21301 ttaccttagt gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac
    21361 cgatatcgac gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg
    21421 atgccagtcc ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga
    21481 cgatgttttt cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca
    21541 cgcccgaaag tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg
    21601 ggatcacatg cagcacacga tgctcatcgc gcacacgcac cgatttcgcg gtatggacga
    21661 cgttttccac atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat
    21721 tctggcagct gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg
    21781 ccatcaattc tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt
    21841 tcaccccgcc tttatccata ccacgtgacg ggcagcttcc cacgccaatg atattgacca
    21901 taccgtcggg cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc
    21961 ctactaccag ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg
    22021 ttgctgagta gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata
    22081 acgcaaatca acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg
    22141 ataaagttct acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt
    22201 aatatcgtta ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag
    22261 agtaaatctg tcctttgcca gcatcggccc catttcgcga tagccctgca acacttcatt
    22321 ggcgctgcct tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg
    22381 cacgctgaag gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg
    22441 cacatattca accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg
    22501 cttaatccac ggcaggcgtt gttctatttg cgtctggatg atgttgacat cttgggtcat
    22561 aaaggtaccc ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta
    22621 atggcgttca ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca
    22681 gcccaacacg acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat
    22741 ccccgccaga cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt
    22801 gttcagagca gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg
    22861 agaagctcat acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg
    22921 gtgaggtatt ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac
    22981 gtccccatcc tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg
    23041 cctcttgtga cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact
    23101 tcgcctcata atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt
    23161 caccgagtat cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt
    23221 catcgtgctg aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg
    23281 acattcccac actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag
    23341 aaatttctgc taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta
    23401 cccacggcgc gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca
    23461 ttgaaagcgc agatgccatc actccgctgc cggtataagg caagcccatc agctcgagca
    23521 tgccctgcag cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa
    23581 aacccatcga cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa
    23641 taccgccttc acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct
    23701 cagcggaagt cccgcccaac aggaccgcga ttttatcagt catgttgttc ttcctccgga
    23761 gtttgcggct tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc
    23821 ccctgaacga gaatcaggtc gttaccggtt aataccggtg ccagcatctc ggctacctgc
    23881 gcagggtccg gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc
    23941 gaacggctgt ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac
    24001 agggtatcaa cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta
    24061 aaacggtgcg gctgaaacag cattaccagg tttttatccg gccagcctgc gcgcgccgct
    24121 ttaatggtgg cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta
    24181 ccgcttttac cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgccca
    24241 gtcccctgga agctctccag cgcacgtaaa atagcctcgt catcaattcc ctcttccgta
    24301 gcaaccgcaa ccgcagctgc ggcgttcagc gcgttatgac gacctggcgc attcagggtg
    24361 acgcgcatcg gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc
    24421 tgataatctt ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt
    24481 cccactcgcg gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg
    24541 taaaacggca ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc
    24601 tggtaggtat ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga
    24661 tgcaggaacg atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa
    24721 cgcgcatgaa cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc
    24781 gcttctgcgt agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca
    24841 atggcgatgc catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc
    24901 ggaatacgcg cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa
    24961 acaacgacca cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct
    25021 cccagattca ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga
    25081 taaccttcat tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca
    25141 aaatgtatgt gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt
    25201 tgtgtattca ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct
    25261 acaattacgc ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa
    25321 tggatgcagc gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca
    25381 gggtgttggc gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg
    25441 ctttttccag cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa
    25501 acaacgccgg tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa
    25561 cgacgacatc cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat
    25621 gctgcggttg ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc
    25681 tctgatgcca gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt
    25741 taagaatgcg tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc
    25801 cagccaaacg ttgctgcggc aacggcagcg ccagtacatc ggtacgcacc gggttaccca
    25861 ccacttccgc attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg
    25921 ccagccattt attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc
    25981 ctaacgacca cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca
    26041 cgtcaggttt gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca
    26101 gcggcgcagc tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat
    26161 caatttcgat gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc
    26221 agcgaacttg ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat
    26281 gtccaccggt tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc
    26341 tcgtacaaac gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat
    26401 gatggctgtc gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag
    26461 acctttggtc ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa
    26521 ccagatgcca atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt
    26581 gcggccaatc gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac
    26641 accgacatac cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg
    26701 cagatactcc agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc
    26761 aaacgccatc agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt
    26821 ccagaatgcg gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc
    26881 tgaaatgccc ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa
    26941 catcgccagc gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag
    27001 taacactgcc aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg
    27061 tacttcgtcg cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt
    27121 cagctccgca ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac
    27181 cgagctaccc actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc
    27241 actgtagcgt tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc
    27301 caggatcaga tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg
    27361 ccctatgggc attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt
    27421 cagccacagt aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc
    27481 catcacccag ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg
    27541 cggcattttc aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca
    27601 aactcattgc ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag
    27661 agcagaacca tatcgcccgg ctgaacacgc ggagccagca agcgcatcgc ctgttccata
    27721 gtttcggttt gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca
    27781 aaacagtaca aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc
    27841 gatttaccat cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac
    27901 gccgcttccg tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg
    27961 tgctccagca ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg
    28021 ctgctggcgc gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg
    28081 tagttatgct gcccggaaag tttcatctct ttcacgttca gcactttctc gcctttcact
    28141 cgcagccagg tttctccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag
    28201 ctgacgcagc gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg
    28261 acacaaactt tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca
    28321 aacggatagc ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt
    28381 aagctggaag tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca
    28441 gcatcaagca gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc
    28501 gccgctttcg ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta
    28561 atcgccacaa tcggtgcttg tgcttcgcgg cagaacagct cgatatcgcc aacgatttcg
    28621 attccggcat cagcggcagc gcttaaggat ggatgcgcca gtgcaatacc gggactggcg
    28681 acaatcagat ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg
    28741 gcttcgggta atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc
    28801 acaccgcgag cgaggaaaaa gtccacgcag gaaagcccag tgaggcccag gccgataatg
    28861 acgacatttt taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa
    28921 tcagaaccag catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc
    28981 ctttcagttc atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt
    29041 taaaggagcc gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa
    29101 tcaccagcag gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg
    29161 ccagcgaacc tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc
    29221 ccagtcctgc cccgactatc gccgtgcaga caatgaccag ttccccggcg tgtcgcagat
    29281 acggtatgtg taaatagctg gcaaagttca tgttgccggt cgcccacgcc accagcgcaa
    29341 aaccaccggc gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt
    29401 ttaccgcgtt gccagtaccc acaatgacga agtaagccag cagaatgtag aacaacccca
    29461 gctgcggcat cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc
    29521 cggcaaggta cagggcgaag gcgataccca gcgcaatgac cgacatccag aaatacttcc
    29581 aacgagcgat caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc
    29641 caataacacc gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt
    29701 aagcccacag cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg
    29761 gcgtaccgcg cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg
    29821 aaagtttttg caaatgagca atcatacgcg ggcccatcca caatgagatg aacagcgcgg
    29881 tcagcaggct gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat
    29941 atttgaccaa atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac
    30001 tacctcttcc atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc
    30061 agcaatcagt gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac
    30121 gccgctggcg gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc
    30181 agcagctttt gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc
    30241 cgccatatcg cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc
    30301 ggcggtcatt gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc
    30361 cagttggatg gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat
    30421 agcatcaagc gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat
    30481 attgtgacgc cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt
    30541 aaattccgta ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc
    30601 attgggtgag aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc
    30661 gttgttgtcg gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc
    30721 tttcgctttc gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt
    30781 gttgaccagc gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg
    30841 gttcgcgcca agttcaataa ctgcgtaatc gtattccggc gttaagcgca acagcgtcat
    30901 cggtacaccg atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct
    30961 taaaatcgcc gccgtcatct ctttaacgga ggttttgccg gaggaccccg tcagagcaac
    31021 cacgcgcgcc ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt
    31081 atccttgacg attaactgcg gcaggtcgat gtccagcgga cggctaacca gtagtgcgcc
    31141 tgcgccgcca gctttcgcct ggtcggcaaa atcgtgggca tcaaaacgtt cgcctttcag
    31201 ggcaacaaac aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt
    31261 gatatctgca ccttgcagtt caccgttgag aatatcggta agttggctaa gggttacgct
    31321 aatcatgcaa tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc
    31381 tgattgccaa caatctggta atcctcatgg cctttgcccg cgaccagtac cacatcattc
    31441 tctttagcct gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca
    31501 tgtccggcat ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta
    31561 cgcgggttat cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc
    31621 agtggacgct tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc
    31681 gcacagtgca ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa
    31741 tccaccacca ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt
    31801 tgcagacgcg cggcggtttt taacagatca gccagcgggt agccaagtgc cagcagtgtc
    31861 gccagcgcga gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg
    31921 ccatcgcccc aacttgaact aaagcgaatg gtcgcgccgc tgtcgtgata gttcacttcg
    31981 gtcgctttca accagcgtcc gtgacagttc ggattgatat gatcttccat tgataccgca
    32041 accgcgtccg gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaacaatc
    32101 gcctgaccac aatgatgttc agaataaagc agccatttcg cggcttcgta gtgttccaca
    32161 tcaccatgat aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc
    32221 aatgccgcca cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc
    32281 gcgccctgat ccaccagccc cgccagctca tgctgaacat cgactgccga accggtggta
    32341 ttttctgtcg ggatcacttt ccccagcaga ccgttaccaa cggtgcccat taccgcgctg
    32401 gtttcgccaa gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg
    32461 gtgcccgtta cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc
    32521 agtgcagata aacgctcgtt gagctggctg agatagatga ccggtacgcc gtgcatttca
    32581 cggatttcac cgtcggtcgc ctcacctttc gcctctgcaa taatggcagc cacaccttgc
    32641 gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac agctacaaag
    32701 agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt
    32761 gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac ctgtcccctc
    32821 gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg gctcgatgtt
    32881 catggtacgc aatacgccgc ccatgatggc accaaagacc ggcgctgaaa cggcgccgcc
    32941 gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc gcggctgact
    33001 cgcaggcgca acgcctgcgg tataagcaat atatttattg atgtagcgac cgtccggccc
    33061 gacctttttc gcggtaccgg ttttaatggc gatacgatag cctttaatcg ccgccttcac
    33121 gccgccgccg cctggtagcg ccacgctttc catcatatgc accacagtgc ggacaatgga
    33181 ttccgggaag acacgttcac cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg
    33241 ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg gtgttaccat
    33301 tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt gtttttgagg
    33361 atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca gtccaaaacg
    33421 tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagcttgg aaacaccgac
    33481 gttactcgac ttctgtaata ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt
    33541 gatttcgtgg ccgttaattc gataaggaat ggtattgagt accgagtttt cccgcaccac
    33601 gccacgttgc aacgcggtca ttaccaccat cggtttaacc gttgagcccg gttcaaacac
    33661 gtcggtgatg gtacggttac gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt
    33721 gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca ccagcacggc
    33781 gctaccagat tcagccttgt taaaggccac cgcgttgttc agttcgcgat aaaccagcgc
    33841 ctgcaggcgt tcatcaatac tcagcgccag gttgtgcgct gcctggctgt cagtagaaga
    33901 aatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac ccggctgccc
    33961 ggtaagccat ttatcgaaac tcttctcaac gccctcaatc ccttgactat cgacgttagt
    34021 aaagccgatg aggtgagcag tcacttcgcc ggacggatag taacggcgag actcttcacg
    34081 cagatgaatc cccggcagtt tcagtttttt gatgtagtcc gccatgtcag ggttcacctg
    34141 acgcgccaga taaataaagc gccctttcgg gttggcgtta atgcgggctg aaagctgatc
    34201 cagcggaata ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc tgataccgcc
    34261 agcgtcatgc acttctttcg ggtcagccca aatcgctttt accggcacgc tcaccgctaa
    34321 cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag
    34381 agaacgcatg tcgccctctt tcaccagcat atccggggag ataacttgta accacgctac
    34441 gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg caaaacgcca
    34501 actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt
    34561 catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg gatcaacatg
    34621 ctgcatttgc agcttttccg tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt
    34681 ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca gcaccagttg
    34741 ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg gttaccacag tcaccgccgt
    34801 caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc
    34861 gataacacca ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag
    34921 agcttctgtc actctgctga tcatgcattc gtcctctctg caatacgcag aactgaacta
    34981 cgggcacgag ggttctcagc cacctcttct tcgcccggca ttaacttgcc tagtgctcgc
    35041 agctgacggc cacccagttt tttgagctgc tcttcagtca tcggtaaccc tgccggaact
    35101 tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg
    35161 aagctgatga tcgaaagccg cccacccggg gccagcacgt tgagcgagct ttttagcgcc
    35221 tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa ggtacgggtc
    35281 gcgggatgtt taaacttatc tttcaccggc gttgcagcag ccacgacttc cgccagttct
    35341 ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg
    35401 gcaaaacgct cttcaccata ggttttcaat acccaggcga tatcggcttc ttctgcggtt
    35461 tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc cagcggacca
    35521 tcgcgcataa aggaaaagcc gcgctcagca tcatcaagtt gcggtgaaga gacgccaaga
    35581 tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta ttcgcccagc
    35641 gcggagaaag gtccgtggat gatggagaag cgcggatcat caatagtctt cgcaacggcg
    35701 atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag ctgcgagagg
    35761 atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg
    35821 atattgaggc cattaacggc ttcatccagc agcaccgtag tatgtttata gttttccatc
    35881 attttataga gacaagtcct gcagtcgctc cgataagtct ccggtagcca actgctctgc
    35941 gtcgatatct tccttgacct gttgatgcca ggttgtttca tcccacagct caaacttgtt
    36001 gaactgtcca accagcatca cttcttttgt cagcccggca tgttgccgca gtactggcgc
    36061 gattaacaat cgacctgcgc catccatctg acattcgctg gcatgaccta acagtaggcg
    36121 ctgcacacgg cgctcaaccg ggttcatgct cgacagacgc gataattttt gctcgataat
    36181 ttcccattca ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac
    36241 catttgaccg gcagcgttct caagcagctg ttcccggtaa cgggtaggca ctgataagcg
    36301 ccctttgctg tcgagattga ctaacgttgc tccccggaac atgccagcct caccccttat
    36361 tcccactttg ccccacaatt tcccactgaa aggagtttac ggagcggagg aaaagcttgt
    36421 caagccagtc acaaggcata ccgaggtaaa aaacccgatg tttacggcta atatcagcgt
    36481 tgtttatttt acgtccaacg tgcgcagcaa attaacgtta tgaatagctg taagaaaaaa
    36541 tccctcatcg tcatcaccgt tttaaaatga ctcactattt tccacagcaa atataaagtg
    36601 tcagtttgcg acgcgagcgg cattttagga catatcttcc ccggttaaca gtccctgttg
    36661 cgtctgtgtg gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa
    36721 atcggcaatt catctctgca tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt
    36781 aattattgtg aatttcagct ggtgagttaa ttaacgggcg ggtttaaaag aacttatcaa
    36841 taatagtcag gaatagtctt atttacttta aggatattga tgtccattcc cgtactctac
    36901 gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc ggcttagcta cggctgagca
    36961 caccgcggcg atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt
    37021 cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag
    37081 ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa
    37141 ttgccaggtc agaaggcagt ttaccatcgc gacgtagcgt gacatccatc accccttgca
    37201 acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg atgcgtttcc agccattttt
    37261 cgaataactg ggcagccgcc tcccgctcat agctgttggc atacaggaaa tgcacttcgc
    37321 gcggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct
    37381 ccggcagcgc gccaagataa agcaccgtct cggcgggaaa cttacgtaac tcttccgcca
    37441 gcatttcggc atcatcctga tcggcaccaa ccacgctggt gaaatgttca cgatcgaggg
    37501 cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct
    37561 caggtggcaa cgacgtcgaa acaataatgg catcaacctg acgctgtaaa aggtgctcaa
    37621 tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc aatcagcagt tgataacccc
    37681 gttgccgcgc ctggcgctca agatagttag cgatgcgggt atagctggtg ttctccagat
    37741 cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt
    37801 tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca
    37861 cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg
    37921 ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata
    37981 gccattgtac tggtatgggt taaattcatt aacatctaag cgcagaaaat taactgcggc
    38041 aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg
    38101 gctgcaaatt gcgcactgag atcattctca acgcattatt ttatcgccgc gcgaaagtcc
    38161 gaccacacca gagcgagcaa cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa
    38221 tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gggtgacatc
    38281 gataatttgc ccacggaata tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct
    38341 ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc gcgccctgcc ccaactcact
    38401 cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg atctgctcaa gcactttttc
    38461 atcgcccacg gtctggatgg tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt
    38521 caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc
    38581 tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg
    38641 cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcactg
    38701 ccatcgacgg taacatcaac aaacaccagg cgattattgc acacctgttc cagcgcctcg
    38761 ctaagtttgc tttccagctc atgcggatga gagatctgga tcccgacgtg accataggct
    38821 tccgccagac ggacgaaatc gggtagtgat tgcatataag attgtgaatg acggccggaa
    38881 tagatcatgt cctgccactg tttcaccatc cccagatagc ggttattgag attcaccacc
    38941 agtaccggca actcgtattg caacgcggta gacagttcct ggatgttcat ctgaatactg
    39001 ccgtcgccag tgacgcaaac cacggtttct tctggcaacg ccattttgac gcccagtgcc
    39061 gcaggtaaac caaaacccat cgtgccgagg ccaccggaat tgatccagcg acgcggtttg
    39121 tcgaatggat aataaagtgc agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa
    39181 gcgtctccct tcgtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
    39241 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatttg ctgccaccag
    39301 tcgcggatct catccagtgg ttgatgggcg gattcttgcg acaagagttc aagcatttgt
    39361 tcgaggacct ggcgagcatc ccccacaatc gggatatccg cagtcacggt tttagaaatg
    39421 gaagtaggat caatatcgat atgcagaaca gtggcatttg ggcagtactt tgccagattg
    39481 ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc
    39541 gtcatattgg cttcgtaggt accgtgcatt cccagcatgc ccagcgcctg acgatgcgtt
    39601 gccggaaacg cccccagccc catcaatgag gaaacaacgg gcagattcaa cgcctccacc
    39661 gtttctttca actgctgatg gcagcccgcc atgattgccc caccgcctac gtagacaacc
    39721 ggtttttttg ccgctaccag cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta
    39781 gtagtgggat tgtaagaacg catactgacc gactcaggcc agacataggg taatttgttc
    39841 gccggattaa gaatatcttt cggtaaatca acgaccactg gtcctgggcg accacttgcc
    39901 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
    39961 ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa
    40021 cctatcaacg aggtcgctac ctgcccggaa aggacaacta atggaatgga atccatataa
    40081 gcggtggcga tgccggtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
    40141 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcactgccgc ctgctcatga
    40201 cgaactaaaa catgatcgat accacccacg gtatgcaatg catcgtaaat atcaaggact
    40261 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
    40321 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
    40381 aaacacatta accgtccgat cttgcttaag caattggcag caagagacat aaacgcaggg
    40441 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacgaagga
    40501 ttaaattgat aataaatcgg ctgaatccca caacttacag caatttctct atttttttaa
    40561 ttaatcctaa aacagagagc ccacacattc agaataaaat tctacattca ataaaaaata
    40621 cagaataaac cagacattca tgcctgacct attctgcatt cagttagcgt ttgcaaattg
    40681 agactaattg ctcttccatc cactgatggc ctttatcgcg cccggcagct tcatgccagg
    40741 agagataaca ggttctgcta ttttgtttta acggcagcgg taatacctgt aattctaagg
    40801 attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca
    40861 ccacgctaag tacgctcatc attgccatgc cctgatacgc gatactggct tgcttatcta
    40921 ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt
    40981 gttcgttata aacatcatgt ttcagtaacg ggcccttaat tgttggatga tttttgctgg
    41041 ctaccagcac catttcatct ttaaataatg gcacgctggt aaattcagga cgatggaagt
    41101 cttcataact aatcacaaac tccgtttcct gataacgcag ctgatgttca gtgttctgat
    41161 ttaatgaaga cttgaacata acatgtatat ttggcgcaat ctgctcaatg tgattataaa
    41221 tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca aagatgaaat acacgttcac
    41281 tgctcgcggg ttcaaaacct gaaccaggca attcattttg tactagttgc aatgcctgac
    41341 gaactgagcc aaaaagttga aatgcgcgag cagtcggttg aataccacgg ccataacgaa
    41401 caaaaagctc gtcattaaac atcaccttca ggcgtgcaac agcgttactg accgcaggtt
    41461 gcgacatacc cagaacatga gcggcacggg taatgttttg ctcctgcatc acggcatcga
    41521 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct
    41581 ctgaatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt
    41641 cccttattgg aatgcagaga aataatcctg aagaatatga tttatcatgc atataaaata
    41701 agaaaaagca aaatgagtaa aattcggaaa acataaagat acgggttctg ataaaaacag
    41761 aatccattgc cgtggaatga gtcatttacc acagcataat aatccataat gtttttgttg
    41821 cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca tctacaaaat agattaaatg
    41881 tgcttattta ataattaatt tatatattta atgcattaat tctcaacatt aattgatcaa
    41941 taatattcac caaatcaata tcaaaaaaaa aaaaaaaaaa aaatcgcaaa acatataatt
    42001 caatacaaat catcaggata ggttttgcaa cgcgtgcatt ttgtcccctt tttcctcgtt
    42061 gattagatgc aaaaatttat gctgaaatat gtcaaccgat gaaaagcgtc ggtagttaag
    42121 cagaaattaa tatcgcttac tttaaccacc gcagcacaat tagctaattt tacgggtgca
    42181 gaactcacgc tggcgggacg tttttattgc gtcagggttg acatccgttt ttgtatccag
    42241 taactctaaa agcatatcgc attcatctgg agctgattta atgactcaca tcgttcgctt
    42301 tatcggtcta ctactactaa acgcatcttc tttgcgcggt agacgagtga gcggcatcca
    42361 gcattaagcc agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt ttttttatgc
    42421 ccgaagcgag gcgctctaaa agagacaagg acccaaacca tgagccagca agtcattatt
    42481 ttcgatacca cattgcgcga cggtgaacag gcgttacagg caagcttgag tgtgaaagaa
    42541 aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga agtcggtttc
    42601 cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca tcgcccgcca ggttaaaaac
    42661 agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata tcgacgtggc ggccgaatcc
    42721 ctgaaagtcg ccgaagcctt ccgtattcat acctttattg ctacctcccc aatgcacatc
    42781 gccaccaagc tgcgcagcac cctggacgag gtgatcgaac gcgctatcta tatggtgaaa
    42841 cgcgcccgta attacaccga tgatgttgaa ttttcttgcg aagatgccgg gcgtacaccc
    42901 attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac caccatcaac
    42961 attccggaca ccgtgggcta caccatgccg tttgagttcg ccggaatcat cagcggcctg
    43021 tatgaacgcg tacctaacat cgacaaagcc attatttccg tacataccca cgacgattta
    43081 ggcctggcgg tcggcaactc actggcggcg gtacatgccg gtgcacgcca ggtggaaggt
    43141 gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc tggaagaagt catcatggcg
    43201 attaaagttc gtaaggatat tctcaacgtc cacaccgcca ttaatcacca ggaaatatgg
    43261 cgcaccagcc agttagttag ccagatttgt aatatgccga tcccggcaaa caaagccatt
    43321 gttggcagcg gcgcattcgc acactcctcc ggtatccacc aggatggcgt gctgaaaaac
    43381 cgcgaaaact acgaaatcat gacaccagaa tctattggtc tgaaccaaat ccagctgaat
    43441 ctgacctctc gttcggggcg tgcggcggtg aaacatcgca tggacgagat ggggtataaa
    43501 gaaagtgaat ataatttaga caatttgtac gacgccttcc tgaagctggc ggacaaaaaa
    43561 ggtcaggtgt ttgattacga tctggaggcg ctggccttca tcggtaagca gcaagaagag
    43621 ccggagcatt tccgtctgga ttacttcagc gtgcagtctg gctctaacga tatcgccacc
    43681 gccgccgtca aactggcctg tggcgaagaa gtcaaagcag aagccgccaa cggtaacggt
    43741 ccggtcgatg ccgtctatca ggcaattaac cgcatcactg actataacgt cgaactggtg
    43801 aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc tgggtcaggt ggatattgtc
    43861 gccaactaca acggtcgccg cttccacggc gtcggcctgg ccaccgatat tgtcgagtcc
    43921 tccgccaaag ccatggtgca cgtactgaac aatatctggc gtgccgcaga agtcgaaaaa
    43981 gagttgcaac gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg atgtcgaaga
    44041 attaccatat tgccgtattg ccgggggacg gtattggtcc ggaagtgatg acccaggcgc
    44101 tgaaagtgct ggatgccgtg cgcaaccgct ttgcgatgcg catcaccacc agccattacg
    44161 atgtaggcgg cgcagccatt gataaccacg ggcaaccact gccgcctgcg acggttgaag
    44221 gttgtgagca agccgatgcc gtgctgtttg gctcggtagg cggtccgaaa tgggaacatt
    44281 taccaccaga ccagcaacca gaacgcggcg cgctgttgcc tttgcgtaag cacttcaaat
    44341 tattcagcaa cctgcgcccg gcaaaactgt atcaggggct ggaagcattc tgtccgctgc
    44401 gtgcagacat tgccgctaac ggcttcgaca tcctgtgcgt gcgcgaactg accggcggca
    44461 tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa gcgtttgata
    44521 ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg catcgcgttt gaatctgctc
    44581 gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa cgtgctgcaa tcctctattt
    44641 tatggcgtga aatcgtcaac gagatcgcca cggaataccc ggatgtcgaa ctggcgcata
    44701 tgtacatcga caacgccacc atgcagctga ttaaagatcc atcacagttt gacgttctgc
    44761 tgtgctccaa cctgtttggc gacattctgt ctgacgagtg cgcaatgatc actggctcga
    44821 tggggatgtt gccttccgcc agcctgaacg agcaaggttt tggtctgtat gaaccggcag
    44881 gcggctcagc accagatatc gcaggcaaaa acatcgccaa cccgattgcg caaattctgt
    44941 cgctggcact gctgctgcgc tacagcctgg atgccgatga tgcggcttgc gccattgaac
    45001 gcgccattaa ccgcgcatta gaagaaggca ttcgcaccgg ggatttagcc cgtggcgctg
    45061 ccgccgttag taccgatgaa atgggcgata tcattgcccg ctatgtggca gaaggggtgt
    45121 aatcatggct aaaacgttat acgaaaaatt attcgacgct cacgtagtgt acgaagccga
    45181 aaacgaaacc ccgctgttat atatcgaccg ccacctggtg catgaagtga cctcaccgca
    45241 ggcgttcgat ggtctgcgcg cccacggtcg cccggtacgt cagccgggca aaaccttcgc
    45301 cactatggat cacaacgtct ctacccagac gaaagacatt aatgcctgtg gtgaaatggc
    45361 gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa tttggcgtcg aactgtatga
    45421 cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg gcgtcacctt
    45481 gccggggatg accattgtct gcggcgactc gcataccgcc acccacggcg cgtttggcgc
    45541 actggccttt ggtatcggca cttccgaagt tgaacacgta ctggcaacgc aaaccctgaa
    45601 acagggccgc gcaaaaacca tgaaaattga agtccagggc aaagccgcgc cgggcattac
    45661 cgcaaaagat atcgtgctgg caattatcgg taaaaccggt agcgcaggcg gcaccgggca
    45721 tgtggtggag ttttgcggcg aagcaatccg tgatttaagc atggaaggtc gtatgaccct
    45781 gtgcaatatg gcaatcgaaa tgggcgcaaa agccggtctg gttgcaccgg acgaaaccac
    45841 ctttaactat gtcaaaggcc gtttgcatgc gccgaaaggc aaagatttcg acgacgccgt
    45901 tgcctactgg aaaaccctgc aaaccgacga aggcgcaact ttcgataccg ttgtcactct
    45961 gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc aatccaggcc aggtgatttc
    46021 cgtgaacgac aatattcccg atccggcttc gtttgccgat ccggttgaac gcgcgtcggc
    46081 agaaaaagcg ctggcctata tggggctgaa accgggcatt ccgctgaccg aagtggctat
    46141 cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt gaagatttac gcgcggcagc
    46201 ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag gcactggtgg ttcccggctc
    46261 tggcccggta aaagcccagg cggaagcgga aggtctggat aaaatcttta ttgaagccgg
    46321 ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg atgaacaacg accgtctgaa
    46381 tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt gaaggccgcc aggggcgcgg
    46441 cgggcgcacg catctggtca gcccggcaat ggctgccgct gctgctgtga ccggacattt
    46501 cgccgacatt cgcaacatta aataaggagc acaccatggc agagaaattt atcaaacaca
    46561 caggcctggt ggttccgctg gatgccgcca atgtcgatac cgatgcaatc atcccgaaac
    46621 agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac gactggcgtt
    46681 ttctcgatga aaaaggccaa cagccaaacc cggacttcgt gctgaacttc ccgcagtatc
    46741 agggcgcttc cattttgctg gcacgagaaa acttcggctg tggctcctcg cgtgagcacg
    46801 cgccctgggc attgaccgac tacggtttta aagtggtgat tgcgccgagt tttgctgaca
    46861 tcttctacgg caatagcttt aacaaccagc tgctgccggt gaaattaagc gatgcagaag
    46921 tggacgaact gtttgcgctg gtgaaagcca atccggggat ccatttcgac gtggatctgg
    46981 aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt taccatcgat gccttccgcc
    47041 gccactgcat gatgaacggt ctggacagta ttgggcttac cttgcagcac gacgacgcca
    47101 ttgccgctta tgaagcaaaa caacctgcgt ttatgaatta atccccttgc ccggtcaaat
    47161 gaccgggctt tccgctatcg tccacgtcat caaacgtctt taacctttgc ggttaaaaat
    47221 aatgcgacaa cagaaataac cgcaattacc cagtagacag caaagtgccc ccaactttgt
    47281 gcaattgctc cctgaataac gccagccaga attaccccgg tagaaatact gttagtaaat
    47341 aaggtggtag ctgcccccgc tcttccaggc attaaatcct gaaaccatag catcccaata
    47401 cccgcaacaa tgccgataaa tacagcgtta aaaagttgca gcgtcatcaa cgccatacgg
    47461 ctatgaaaga aaatcaatcc ggtgtaaaac agtactcctg ccgccactgc tatgaccatc
    47521 attcgccgct taccataacg tttgacatag tagccagcca gaatcattgc tggtatttcc
    47581 agtccagctg ccgtccccat caggaaaccc gccagtttgt ctggcaatcc taactcgcta
    47641 ctgatccaca acggcatatc aataatgtac atggtgttgc aggtccacat taacgtcgag
    47701 gcgacaaata acatccgtac gttactatcc tgccagccac cttgcattga taaagcattt
    47761 tccgacggca gttctacccg cgccacagac ggaagcataa atgcaatcaa taccagactg
    47821 agtgtgaata tcccggcggc aatcgaaaac atcaccgtaa agccgtaatt caacgccagc
    47881 ataaaggcca acggtggacc gataacccat gccagagaaa gctgcgcacg catcaccgag
    47941 ctaaacatca ccacttctcg cgccgagtta tccgcatatt cccgcgccag agcaaataac
    48001 tgtggcattg ccgtattggc cagagatgcc agaagcacac cacaggtgat aagcgtcaga
    48061 taatgacgat taaatgcaaa caatagcgca ttgccgatag ccatcaaaca gcaaaatata
    48121 atcagttttc gccgatcgcc ctgactgtca gaacgttttg ccaaccagag gcttacgccg
    48181 atcccagcaa tagcattcac cgtataaaag agtccgatcc agaaaggttg cgcgccaacc
    48241 tcacgactca gaaataagct caatgtagga gcctgtagcg ccccggccac ccccatcata
    48301 aaagcgacca gcataaatgc cgcgtaaaca ccgttcatac ggcgagccat cgtcattatc
    48361 cagatcatac gttccctttt tagcgcggcg agaataaaaa aaaccagcag gtataatctg
    48421 ctggcgggtg attttacacc aatactcagt cacacatgat gcaggcaagt caactttcag
    48481 aattgcggtc atcccactgc atcagttctt caagcatggt taatcgttgt gggacgctta
    48541 accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa aactgacgca
    48601 tggggcacct ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt
    48661 gctgtctttt gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca
    48721 tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag
    48781 cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg caggatcgcg
    48841 gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct
    48901 ataccgggct ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg
    48961 accaactagt gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc
    49021 tgggccgcag cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc
    49081 gtaatctgct acctggcagc gcattgcgcc gttccgaaac ccatattgcc cggcaaatct
    49141 tcagttcgct aacgcgcata aatgaggaaa atggggaact ggaagcagac atcgcccacc
    49201 actggcagca aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc
    49261 atcatggtcg tgaactggaa atggacgacg tgatcgcctc tttaaaacga atcaatacgc
    49321 tgccgctcta ttcgcatatt gcggacattg tgtcgccgac gccctggacg ctggatatcc
    49381 atctcacgca accggaccgc tggttaccgt tactgctggg acaagttccg gcgatgatcc
    49441 tgccgcgcga atgggaaacc ctcagtaact ttgccagcca tcccatcggc accggtccgt
    49501 atgcggtgat ccgcaacacc accaatcaac tgaaaattca ggcattcgat gacttcttcg
    49561 gttaccgggc attaatcgat gaagttaacg tctgggttct gccggaaatt gccgacgagc
    49621 cagctggagg tctgatgcta aaaggtccac agggtgagga aaaagagatt gaaagccgcc
    49681 tggaggaagg ttgctactat ttactgttcg acagccgcac ccatcgcggg gcgaatcagc
    49741 aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg
    49801 aacagtacca gcaactgtgg ttcccggctt atggactgct cccccgttgg caccatgccc
    49861 gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct catcctgacc ttttatcagg
    49921 atcacagtga gcatcgggtg attgccggga tcatgcagca gattctggca agtcaccagg
    49981 tcacgctgga aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg
    50041 atatctggct taacagcgcc aactttacgc tgccgctgga tttttcgctg ttcgcgcact
    50101 tgtgcgaggt gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc
    50161 gctggcgcaa tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagtaaag
    50221 caatggtgcc gcttatccac cactggctga tcattcaggg acaacgcagt atgcgcggcc
    50281 tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccgccggatc
    50341 catgattgct ggtagataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg
    50401 caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga
    50461 tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa
    50521 tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt
    50581 tatacctacg attccttcgc cgccgactgg gggcctggtc caaagatcaa aaaagctttt
    50641 gaagccgact gtaactgcga actgaaactg gtggcgctgg aagatggcgt ttcgcttctc
    50701 aaccgtctac ggatggaagg caaaaatagt aaagccgatg tggtgctggg gctggataac
    50761 aatctgttag acgccgccag taaaaccgga ctatttgcca aaagcggtgt ggcagcggat
    50821 gccgttaacg ttcccggcgg ctggaataat gacactttcg tcccgtttga ttacggctat
    50881 ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg
    50941 gttgagagcg atcagaactg gcgggtgatt tatcaggatc cgcgcaccag tacgccgggg
    51001 ctgggcctgc tgttgtggat gcaaaaagtc tatggcgatg acgccccaca agcctggcaa
    51061 aaactggcga agaaaaccgt cacggtcacc aaaggctgga gcgaagccta cggcctgttt
    51121 ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc
    51181 gaagagaaaa aagataacta cgccgccgcg aactttagcg aaggtcacta tctgcaggtg
    51241 gaagtcgccg cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag
    51301 tttatggttt ctccggcttt tcagaatgcg atcccaaccg gtaactggat gtatccggtg
    51361 gcaaatgtca cgctgcctgc cgggtttgaa caattgacca aaccggccac cacgctggag
    51421 ttcacaccag ctgaagtggc ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc
    51481 gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtggtag
    51541 cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg
    51601 cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc
    51661 tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc
    51721 gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc
    51781 tagttgctgt tttcggcatt cttagcgtct atggtcgtca gggctggctg gcatcgctct
    51841 gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc attttgctgg
    51901 cgcacgtatt ttttaatctg ccgatggcga gccgcttatt actccaggca ctggaaaaca
    51961 ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc
    52021 gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg cttatcttta
    52081 tgctctgttt cgccagcttc gccaccgtgc tatcgctggg cggcggtccg caggcaacta
    52141 ctatcgagct ggctatttat caggcgctga gttacgacta cgatcctgcc cgcgcggcga
    52201 tgctggcgct gctccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa
    52261 gtaaggccat tgcgcccggt accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc
    52321 tgcatagccg catttgcgac acggtgttaa tagtgctggc gctgctgctg ttgctgccac
    52381 cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac
    52441 cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggtgtattgt
    52501 gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa
    52561 tgctggcggg tcaggcgctg gagatgagcg gcatgttgat cctcgccatg ccggggattg
    52621 tgctggctac cggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg
    52681 acggcattgt gattttcacc aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg
    52741 aaaacccgat gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg ctgggcattg
    52801 aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg
    52861 cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta
    52921 acgatgattt ccgtaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc
    52981 aggacggcgc ggtcaccgcg ttaattctgc tactactctg ttttctgctg tttaccgtga
    53041 ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct ttatcaccat
    53101 ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcaat cctcgggcca
    53161 agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc
    53221 ggttcgctga ctatcgacag cgttgatcac accacgacgc cgccgtcacg ccgtccggta
    53281 tcgatgctgt ttcaggagaa caacctgttc agccacctga cggtcgcgca gaacatcggg
    53341 ctggggctta atccgggatt gaagctgaac gcggcgcagc aggagaaaat gcacgctatc
    53401 gcccgccaga tggggattga taatttaatg gcgcggttac cgggcgagct ttccggcggt
    53461 cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat tttattgctc
    53521 gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgctgac gctggtgagc
    53581 acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg
    53641 gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc gcatcgcctg gcagggtaag
    53701 accaatgagt tgttgagcgg taaggcgagt gcttcggcac tattggggat tacgggttag
    53761 tatgcactgt cggatgcggc gtgaacgcct tatccgacct acgggtagtg cgttttgtag
    53821 gcctgataag acgcggtcag cgtcgcatca ggcaacccgc acaagactat ctcctaaccc
    53881 ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc
    53941 accagcgcca ccacgccgat ggcagaaatt aacggcgtca gccacaacaa acggccgcgc
    54001 gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc
    54061 cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc
    54121 tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac
    54181 ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc
    54241 agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga
    54301 atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt
    54361 ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag
    54421 atccagtcgc ccagcagaca accgacaatc cctgccagcc aggcgtgcca gaaacttaac
    54481 tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg tagaatcaaa
    54541 ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg
    54601 gtggattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga
    54661 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
    54721 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
    54781 gctcgggctg gccccggtgc attttttaaa tactcgcgag aaatagagtt gatcgtcaaa
    54841 accaacattg cgaccgacgg tggcgatagg catccgggta gtgctcaaaa gcagcttcgc
    54901 ctgactaatg cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
    54961 aagatgtgac agacgcgacg gcgacaggca aacatgctgt gcgacgctgg cgatatcaaa
    55021 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtacac gattatccat
    55081 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
    55141 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
    55201 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc
    55261 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
    55321 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg
    55381 gaacagcaaa atatcacccg gtcggcagac aaattctcgt ccctgatttt tcaccacccc
    55441 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
    55501 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa
    55561 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
    55621 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
    55681 ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
    55741 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
    55801 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
    55861 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
    55921 tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg
    55981 tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt
    56041 atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc
    56101 cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg
    56161 tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
    56221 cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg
    56281 cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt
    56341 tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt tattccagcg
    56401 aatggttctg ggcaaaaatc ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg
    56461 ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt accacccgcc
    56521 cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg cacgaaagct
    56581 ggggcggcct gccgccagcc agtttctttg atgagctgga cccgctcctc aatcgccatt
    56641 tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc accttatgcc
    56701 cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg
    56761 actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta aaagttatcg
    56821 gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag cgggcagtga
    56881 aaggtatttg cggtcaggtt gatggcagcg tggtgcctgg atttatcggt ctggaagcag
    56941 gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc tggccgctgg
    57001 aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc cagaaacaac
    57061 tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg
    57121 tgctcgactg gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga
    57181 ttaccgatct taacctcgct accgacgctc cgctgctgtt cggcggtttg attgctgcca
    57241 ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc gccgtcaata
    57301 acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg
    57361 acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg
    57421 cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccctcagcc cagcaaaaaa
    57481 tggccagtgc ggtagagaaa accctgcaac cgtgcagcga acaggcacaa cgctttgaac
    57541 agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt
    57601 ccgccccggc acaggctgcc caggccgttc cgactctata aggacacgat aatgacgatt
    57661 tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg cccggaaacc
    57721 ctgcgtcagg tcacccaaca tgccgagcac gtcgttaatg cgctgaatac ggaagcgaaa
    57781 ctgccctgca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat caccgctatt
    57841 tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct gcacaccttc
    57901 tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt gctgcaattc
    57961 cacacccagt ttaacgcggc gctgccgtgg gacagtatcg atatggactt tatgaacctg
    58021 aaccagactg ctcatggcgg tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa
    58081 catggcgtcg ttaccggtca ctggcaggat aaacaagccc atgagcgtat cggctcctgg
    58141 atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg atttggcgat
    58201 aacatgcgtg aagtggcggt caccgatggt gataaagttg ccgcacagat caagttcggt
    58261 ttctccgtca atacctgggc ggttggcgat ctggtgcagg tggtgaactc catcagcgac
    58321 ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac gcctgcaaca
    58381 caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga gctggggatg
    58441 aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga agatttgcac
    58501 ggtctgaaac agcttccagg tctggccgta cagcgtctga tgcagcaggg ttacggcttt
    58561 gcgggcgaag gcgactggaa aactgccgcc ctgcttcgca tcatgaaggt gatgtcaacc
    58621 ggtctgcagg gcggtacctc ctttatggag gactacacct atcacttcga gaaaggcaat
    58681 gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgcagt agaagagaaa
    58741 ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc ccgcctgatc
    58801 ttcaataccc aaaccggtcc agcgattgtt gccagcctga ttgatctcgg cgatcgttac
    58861 cgtctgctgg ttaactgtat cgacacggtg aaaaccccgc actccctgcc gaaactgccg
    58921 gtggcgaatg cgctgtggaa agcgcaaccg gatctgccaa ctgcttccga agcgtggatc
    58981 ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc
    59041 cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac ccgcctgcca
    59101 gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc
    59161 acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct acgcgctgtg
    59221 tttttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg
    59281 cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcaaagt gcatccggca
    59341 cgaaggagtc aacatgttag aagatctcaa acgcctggta ttagaagcca acctggcgct
    59401 gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg
    59461 cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat
    59521 ggtcgtggtt agcatcgcaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctctga
    59581 cacgccaact caccggctgc tctatcaggc attcccgtcc attggcggca ttgtgcatac
    59641 gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag caaccggcac
    59701 cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga
    59761 aatcaacggt gaatatgagt gggaaaccgg taacgtcatc gtagaaacct tcgaaaaaca
    59821 gggtatcgat gcagcgcaaa tgcccggcgt cctggtccat tctcacggcc catttgcatg
    59881 gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat
    59941 ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga
    60001 taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa
    60061 aaccacagcc aatcaaacga aaccaggcta taatcaagcc tggtttttta ttggattttc
    60121 aacgtggcgc aggcaggttt tatcttaacc cgacactggc gggatacccc gcaagggaca
    60181 gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa
    60241 gagtccgtgg cgtttattcc tgccgatcag gttccccgcg ctcagcatat tttgcagggt
    60301 gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat
    60361 ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt
    60421 ggcgttaccg tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt
    60481 atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt
    60541 ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc
    60601 acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat
    60661 atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc
    60721 agccgcccgc tgctgctgga aaaactcaac gcctggtttg caaaccacga tcccgatgtg
    60781 atcatcggct ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt
    60841 taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc
    60901 tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag
    60961 gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag
    61021 ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc
    61081 cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg
    61141 acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgagc aacggtgaac
    61201 ggcctgccgg tggatcggca cggcggttcg gtggcagcgt ttggtcatct ctattttccg
    61261 cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccacc gcacgccagc
    61321 cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac
    61381 tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtt
    61441 gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga tgcctggttc
    61501 tcacgggaga aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa
    61561 gccaaacgtc agggtaataa accactttcg caggcgctga agatcattat gaatgccttt
    61621 tatggcgtgc ttggcaccac cgcctgccgc tttttcgatc cgcgcctggc atcgtcgatc
    61681 accatgcgtg gtcatcagat catgcggcaa accaaagcat tgattgaagc gcagggctac
    61741 gacgttatct acggagatac cgactcgact tttgtctggc taaaaggtgc acattcggaa
    61801 gaagaggcgg cgaaaatcgg tcgtgtactg gtgcagcacg ttaacgcctg gtgggcggaa
    61861 acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac tcatttctgc
    61921 cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ctacgccggg
    61981 ctgattcagg agggcgataa acagcggatg gtgtttaaag ggctggaaac cgtgcgcacc
    62041 gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat cttccgcaac
    62101 gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg tgaactggat
    62161 gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg
    62221 ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg cggtcgcccc
    62281 ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg cccggagccg
    62341 ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca gctacaaccc
    62401 gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat gaccgggcaa
    62461 cttgggctat tttgagcaaa aaaaagagtt cgccagatac cattttgatg cgtgacgaat
    62521 gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa caccactacc
    62581 tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc cgaacacata
    62641 tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg ggacttggaa
    62701 ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact ggtgaaaacc
    62761 gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct ggtgatacta
    62821 ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc
    62881 tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg cgtgaagttt
    62941 tccttgatag caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg
    63001 accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc
    63061 gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gattccgcat cagctcaaca
    63121 tcgctcatga tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt
    63181 tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct ggcgctgctg
    63241 aacgtgtgct gattatcgtc ccggaaacct tacagcatca gtggctggta gaaatgctgc
    63301 gccgtttcaa cctgcgcttt gcactgtttg atgatgagcg ttatgccgaa gctcagcacg
    63361 atgcttacaa cccgtttgac accgaacagc tggtgatttg ctcgctggat tttgcccgtc
    63421 gtagcaaaca gcgcctggaa catctctgtg aagccgaatg ggacctgctg gtggtcgatg
    63481 aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag gccattgaac
    63541 aactggcaga gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa cagctgggga
    63601 tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc
    63661 agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc atgctgctgg
    63721 caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc ggcgagcagg
    63781 atatcgagcc gctgttgcag gcagcaaaca gcgacagcga agatgcccag agcgcccgtc
    63841 aggagctggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca
    63901 cgcgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaaa ctgccgctac
    63961 cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt aaaagtgcgg
    64021 aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt gaaggtgata
    64081 acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac ctgaccagcc
    64141 atcgctctca gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg caactggagc
    64201 aggtactgcg cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt atgtcgatta
    64261 tcgaacgtga ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca caggtactgc
    64321 tgtgctcaga aatcggttct gaaggacgta acttccagtt cgccagccac atggtgatgt
    64381 ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg gatcgtatcg
    64441 gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct cagtcggtgc
    64501 tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg accggacgca
    64561 ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg gatcaaaccg
    64621 aaggctttga cgatctgatc aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc
    64681 tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa aaagcccagg
    64741 cactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc ttcgccatga
    64801 acctgttcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg atcgtgctga
    64861 cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat ggcatcacca
    64921 tcacctttga tcgtgaagtg gcactggcgc gtgaagatgc acagtttatt acctgggagc
    64981 atccgctgat ccgcaacggt ctggatctga tcctttctgg cgataccggt agcagcacga
    65041 tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa ctgatttatg
    65101 tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca ccgacgccgg
    65161 tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag tttgaaacct
    65221 ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt aacgccgtgc
    65281 agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa tctgcccgtg
    65341 cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc
    65401 tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc gccattgaga
    65461 gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ctggcgtctg gatgccctgc
    65521 gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa actacaatcc
    65581 accgcaggaa ccctggttgg ttatcctgta tcaggatgac cacattatgg tggttaacaa
    65641 gccgagcggt ttgttgtctg tgccgggtcg tctggaagag cacaaagata gcgtgatgac
    65701 gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata tggctaccag
    65761 cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac gccagttccg
    65821 cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat cccccgcaga
    65881 aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac agaaagtctg
    65941 ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg cggcggataa
    66001 cacggcaaga gtggtgttaa aaccgattac cgggcgttcg catcaattgc gtgtgcatat
    66061 gctggcgctg ggtcatccga ttctcggcga tcgtttttat gcatcaccag aagcgagagc
    66121 gatggcacca cgtttgttgc tacatgcaga gatgttgacg attacccatc cggcgtatgg
    66181 caatagtatg acgtttaaag cgccagcgga tttttaatcg tcacgctggc gcagttcggt
    66241 aggcggataa ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct gatgcgacgc
    66301 gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct gctgctttat
    66361 cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctcagg
    66421 cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct tacggtaggc
    66481 acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac aggcatcttc
    66541 cagcgtcggg ccacgctgcg cttgctgcca gttaccaccg ccagattgct gctgataacc
    66601 gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gggcgcggga
    66661 gatccctaat tcttcagcaa tgacatacag caccgcccgt tcattcgggt gcagtgaacc
    66721 atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc
    66781 aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattat ctgattttcc
    66841 cactcggaac gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat
    66901 caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg
    66961 ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa
    67021 ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac
    67081 tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca
    67141 atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc acaatataaa
    67201 ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata atttgcatta
    67261 tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca
    67321 ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga
    67381 tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga
    67441 ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca cagtgcatgt
    67501 tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga
    67561 ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg
    67621 tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag
    67681 aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat gtccattacg
    67741 acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata
    67801 ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggtcgcggt aaagcggacc
    67861 tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc tttacctcct
    67921 gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag
    67981 aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg atcttttata
    68041 gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg
    68101 ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg aacatcgcgc
    68161 caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac atcatgtggg
    68221 agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc
    68281 cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttat
    68341 tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc gactacacca
    68401 aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg
    68461 gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt
    68521 caaccaagca gttccaggtt ttcagcgaac agaacaccag tagctactcg gcagagccgc
    68581 agttagacgt taattactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc
    68641 aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg
    68701 aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt
    68761 tgctggcaac ccattatcag caaaccaatc ttgactggta taactccaga aacacgacca
    68821 agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc aaaatggtct
    68881 ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc
    68941 agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctgc
    69001 tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg
    69061 cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg
    69121 aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg
    69181 acaacataac atgggagaat gacgacaaaa cgggttcact ggtgtgggca ggcgatactt
    69241 actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg
    69301 ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac
    69361 agctgaatta ccgttacgcc agcccggaat atattcaggc tacgctgcct aagtactatt
    69421 ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc agctggccaa
    69481 ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg aacaagcaag
    69541 ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg
    69601 agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg
    69661 gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc
    69721 tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc
    69781 cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt
    69841 atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc
    69901 gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa
    69961 ctgaacgctg ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg ccaccaaatc
    70021 atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa
    70081 atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg
    70141 ctggatcaga tgcgcagccg tctggcttac gatggactga actacaacac ctatcgtaac
    70201 cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc
    70261 accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc
    70321 agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacctctgat
    70381 caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg taacggcgct
    70441 gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa cggcggccag
    70501 atgggctggg gccgtattca ggagttgccc gggatcttcg cccaggcatt aagcaccgcg
    70561 aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat tcttaaagtt
    70621 aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt
    70681 ctgctgaaac cgtcgccgat catgactgac gaacaggccc gtgtgaaact ggaacagatt
    70741 gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctctcag
    70801 gatccaggct ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat
    70861 ccggccttcc gtgacgccct gactcgcctg aacaaaggtc aaatgagtgc accggttcac
    70921 tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac
    70981 gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc ggaagaagca
    71041 gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag caactaatgg
    71101 ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag
    71161 ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc
    71221 tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca
    71281 actcccctgc acaaccgcaa actacgggca cattaacgct acttcctgtc gcgctacgtg
    71341 aatctgtcac tgcggggcag ttagcgattg aaaatggaca ttacgtggtg gagacgctgg
    71401 cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc
    71461 ataaaggcgt cattaacgac gcaggcattc cgtttaccgg tcataccgag tttttcgaag
    71521 agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc
    71581 tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcacttttgc
    71641 acgaagtgat tgctattttg catcacgatt tgcgtacaaa atttggtatt gccgaaccgc
    71701 gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag
    71761 agatagacac cattattccg gtgctcgacg agctgcgggc gcagggaatg aaactcaacg
    71821 ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc
    71881 tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc gggcgcggtg
    71941 tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg
    72001 aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca
    72061 tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa
    72121 acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat
    72181 taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg cattgaccga
    72241 gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg atctggcggc
    72301 acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc aggatgcgat
    72361 gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa
    72421 cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat
    72481 tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa
    72541 cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccggt
    72601 actggaagtt ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg
    72661 cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgtgtgt tgagccgcat
    72721 caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt
    72781 tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg aaaatatctc
    72841 tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag
    72901 ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca
    72961 caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg
    73021 gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt
    73081 gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa
    73141 gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac
    73201 tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc
    73261 gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat
    73321 ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc tgggaaagat
    73381 accctctggc tgacgggtga tctggtcgcg cgcgggccgg gttcgctgga tgttctgcgc
    73441 tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
    73501 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg
    73561 gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagccttt gctgcaaatc
    73621 gacgaagaga aaaagttggt gatggcccac gcagggatca cgccgcagtg ggatctacag
    73681 accgccaaag agtgcgcgcg cgatgtagaa gcggtgctat cgagtgactc ctatcccttc
    73741 tttctcgacg ctatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg
    73801 ggaagactgc gttttatcac caacgccttt acccgtatgc gtttttgctt tccaaacggt
    73861 caactggata tgtacagcaa agaatcgccg gaagaagccc ctgccccact gaaaccgtgg
    73921 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg
    73981 ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg ataccggctg ctgctggggt
    74041 gggtcattaa cctgcctgcg ctgggaagat aagcagtatt ttgtccagcc gtcgaaccgg
    74101 cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatatagg aaggccggat
    74161 aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaaa ttaccgccgc
    74221 tccagaatct caaagcaata gctgtgagag ttctgcgcat cggcgtcgtg gaattcgctg
    74281 aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct
    74341 gcgtcgatat gcgtcagata cagtttttgc gcttttggca ggaactgctc ataaacgcga
    74401 ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcatccacc
    74461 gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg
    74521 cgtcctggca acggacgacc gattgattcc caggtatggc ggcccataat cacgggttta
    74581 tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt
    74641 tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat tgagatttcc
    74701 cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac tggtgcgagg
    74761 atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctctcc ctggactcgc
    74821 cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttac gtcactcttt
    74881 attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt ttaccttctt
    74941 cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcgatcc tcggtaatga
    75001 tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg gtatcattct
    75061 caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca tcggcacgtt
    75121 ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg gttttcagcg
    75181 caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa cgaatgtagt
    75241 ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct ttcaccatct
    75301 ctgtcagttg caggttggtt tgcggatcgt cgatggcgtt aatcagcact tccgctttcg
    75361 ccgctccggc agattccagt aaatccatcc gcgtggcatc gccataaaac actttcatac
    75421 caaatttacg caaggtttcg atatggtccg gatcgtgatc gagtaccacc attttcaccc
    75481 cgctggagag cagtaaacgt ccggtaatct gcccaaaacg accgaatccg gcgataatca
    75541 cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ttcctcagta gaagattgct
    75601 caaggcgatt gaggatcacc agcagaatcg gcgttgctgc catcgacagc gccaccgcca
    75661 gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc gcgccaaata
    75721 ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccaacga cgctgtttat
    75781 ttggcacttg caacggtcgg gcaatcagcc acagcatggc gattttgatg atgaggaaac
    75841 cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag cgtgccaaag tctatcgaca
    75901 tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaatggttcg atatcgctct
    75961 ccagcgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac gcgcccatcg
    76021 ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacgagg aataacgcca
    76081 cggcactaaa cacttcccgc aagccagagc gggctacaaa acgcagcgcc ggacgcgtga
    76141 catagcgccc cagcaatacc accagcacca gcgcacccgc cacttttaac gccgagagag
    76201 caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag tagcggaatc atcgccacca
    76261 gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga cccatttgcg
    76321 tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag gagagcgcca
    76381 gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagtaacatg cagagcagcc
    76441 ccagcaggcc gccgcaaatc accatctgca atgcgccacc gccgaacact gccgcacgca
    76501 gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc agcaccacgc
    76561 caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc ccccacgggc
    76621 caataatgaa gccggcgatc aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa
    76681 tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc ctgaatcagc gtatggctat
    76741 ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtgacgcgc ctgcccttcg
    76801 agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag ccagttcagc
    76861 ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc cggatgcgca
    76921 ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa atgtttgcca
    76981 tgcagcgccg tgccgccgtg accgtaagcc cagccgtgcg agaaaacttt atcgatccaa
    77041 agtttgagga gcggtggaat gctgtaccac tgcatcgggt gctgccagac gatcagatcg
    77101 gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg atagagttga
    77161 taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat ccgtttattc
    77221 gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg tagcccgcgt
    77281 gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    77341 atattgattc agaaaagaga gaaatctgaa caatttaccc cattctctgc ccgcggatcg
    77401 tcatattgct ggtttgcgcc agtgacatcc ctcgagtctc cggggcaaac gctacggaaa
    77461 tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat
    77521 tattgataaa gatcggcagt gcccaggtcg aaacaatggt gccaatacgg cttaaggaca
    77581 taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta ggatagagcc
    77641 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca
    77701 gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc atcatggcaa
    77761 agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc ggcggaatac
    77821 agccgagcat aaagaacagg ctaatcacca cgttccccag cgccgcgttt ttgccaacac
    77881 ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca
    77941 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    78001 gatcgcgaaa acgggtttgc tgcggttgct cttcatcgaa agccaccggt tcgccaaaca
    78061 gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac cagcggggag
    78121 attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc gcgctaccca
    78181 gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga tcggcgcagg
    78241 tcgcgccgac ataccacatg gcggcgataa agctgatgga aaacgcccgc tgacgggtac
    78301 tggagaactc ggtgatcatt gaggtggcga tgggataatc tgcaccgatg acaatgccga
    78361 taagtacccg catcaccaac agttcgacgg gggatgaaac aaacatcgtc gccaccgata
    78421 tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaaa
    78481 tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccaacaagc
    78541 caatccagtc agcgtccagt tttagcgccg gagtcagttg ctccagcgcc acgccaatca
    78601 ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt
    78661 gaattgagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt
    78721 tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc gtaacggaac
    78781 tccacaccaa aggtgccgcg cgggtatttc cactgttcca gcgccgaccc cagccccaga
    78841 atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc
    78901 tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagggc ctgtttatcg
    78961 gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc
    79021 agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct
    79081 tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg cgcatcagtt
    79141 ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agttccgggt
    79201 agccgctaaa catgcgtggg ttatcaagga aggccggtag tttctggtac atacgcatat
    79261 cgcgcagcgg gccactctca agatgctgac gatattccgc cagtttttgc ttactgaaat
    79321 cgtcgctttt catcgctgaa agcacggttt ttgctgcggc ttccccggcg gcaatcgcca
    79381 gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca tcaccggcaa
    79441 tcaatacgcc gtcaccaacc aactccggca gcatgttgat gcctgcttcc ggcactacgt
    79501 gagcggaata ttccaccagc ttgccacccg cgatcagcgg tgcaacggcc ggatgttgtt
    79561 tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc atgcagatga tgcagaccac
    79621 aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc
    79681 catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac
    79741 ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg
    79801 tcggtttaac gcgttttgcc atccccagct tttcggcaag gatggagttc actccatcag
    79861 caaggatcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg acgactttgc
    79921 catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg cccgcttctt
    79981 cggcctgctc catcagccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg
    80041 atggcgaggt ttcgtcacca ttgcagtagt ccatagtcat cgctgacttt tccgtcataa
    80101 acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggagtcggcg aaaccaggaa
    80161 taatgtgttc caggctgtgg gcatagagac gcccgccggt gacgttcttg gcacctgcgg
    80221 aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa
    80281 ccgaaccggc aagcccagca ccgacgatga tggcgtcaaa gatatcttcg gacataagag
    80341 ctccctgtca gcggcagggc caaaatgcag ccctgccaga gtggatcaac gcgctaaagc
    80401 tgcggtcagc gccggaagga tcttcacggc gtcaccaaca atgccgtaat ccgcgtactg
    80461 gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgatg cattagcacc
    80521 aaccatgtgc tggatctgcc cggagatccc caccgccagg tacagttcag gtttcagcat
    80581 caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt ccgccaccgg
    80641 acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt
    80701 ctctttgctg ccaataccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac
    80761 gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca
    80821 ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc
    80881 gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc
    80941 accgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg tgctggcatc
    81001 gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg
    81061 ggtgtttggc agcagcacca ggccgtctgc gccgtgctgg cgaatagtgt cagccatgac
    81121 accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt
    81181 agcgccgagc tggattgcct gtacgccgtc ggcatcattg aggacaaagg tgttgatttg
    81241 attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg tatcgctgaa
    81301 tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga
    81361 ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgt
    81421 tcgcgctgtt tcggcgcggc aacctgttgt tctgaccagg ctgcctctgc gttaaaacca
    81481 atatccgccg ccgaccatac ctggacgggc tttttc
//
LOCUS       DAHBHC010000010        81103 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHBHC010000010 DAHBHC010000000
VERSION     DAHBHC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.010"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(358..734)
                     /gene="rnpB"
                     /locus_tag="M2A35_000894"
     ncRNA           complement(358..734)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="M2A35_000894"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(767..1912)
                     /gene="garK"
                     /locus_tag="M2A35_000895"
     CDS             complement(767..1912)
                     /gene="garK"
                     /locus_tag="M2A35_000895"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCB7197229.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2009..2899)
                     /gene="garR"
                     /locus_tag="M2A35_000896"
     CDS             complement(2009..2899)
                     /gene="garR"
                     /locus_tag="M2A35_000896"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCB7197230.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2929..3699)
                     /gene="garL"
                     /locus_tag="M2A35_000897"
     CDS             complement(2929..3699)
                     /gene="garL"
                     /locus_tag="M2A35_000897"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCB7197231.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3715..5049)
                     /gene="garP"
                     /locus_tag="M2A35_000898"
     CDS             complement(3715..5049)
                     /gene="garP"
                     /locus_tag="M2A35_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCB7197232.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWVWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5424..6995
                     /gene="garD"
                     /locus_tag="M2A35_000899"
     CDS             5424..6995
                     /gene="garD"
                     /locus_tag="M2A35_000899"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCB7197233.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7144..7479
                     /gene="prlF"
                     /locus_tag="M2A35_000900"
     CDS             7144..7479
                     /gene="prlF"
                     /locus_tag="M2A35_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCB7197234.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7479..7943
                     /gene="yhaV"
                     /locus_tag="M2A35_000901"
     CDS             7479..7943
                     /gene="yhaV"
                     /locus_tag="M2A35_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCB7197235.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7998..8807)
                     /gene="agaR"
                     /locus_tag="M2A35_000902"
     CDS             complement(7998..8807)
                     /gene="agaR"
                     /locus_tag="M2A35_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCB7197236.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIASLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9056..10336
                     /gene="kbaZ"
                     /locus_tag="M2A35_000903"
     CDS             9056..10336
                     /gene="kbaZ"
                     /locus_tag="M2A35_000903"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCB7197237.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYTSANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARAMLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVTLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10359..10832
                     /gene="agaV"
                     /locus_tag="M2A35_000904"
     CDS             10359..10832
                     /gene="agaV"
                     /locus_tag="M2A35_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCB7197238.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGSAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10843..11622
                     /locus_tag="M2A35_000905"
     CDS             10843..11622
                     /locus_tag="M2A35_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCB7197239.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11612..12490
                     /locus_tag="M2A35_000906"
     CDS             11612..12490
                     /locus_tag="M2A35_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCB7197240.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12508..12942
                     /locus_tag="M2A35_000907"
     CDS             12508..12942
                     /locus_tag="M2A35_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCB7197241.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12939..14072
                     /gene="nagA"
                     /locus_tag="M2A35_000908"
     CDS             12939..14072
                     /gene="nagA"
                     /locus_tag="M2A35_000908"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCB7197242.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAQPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14423..15577
                     /locus_tag="M2A35_000909"
     CDS             14423..15577
                     /locus_tag="M2A35_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCB7197243.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15590..16450
                     /gene="kbaY"
                     /locus_tag="M2A35_000910"
     CDS             15590..16450
                     /gene="kbaY"
                     /locus_tag="M2A35_000910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCB7197244.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16617..17093
                     /gene="agaB"
                     /locus_tag="M2A35_000911"
     CDS             16617..17093
                     /gene="agaB"
                     /locus_tag="M2A35_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCB7197245.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17132..17935
                     /gene="agaC"
                     /locus_tag="M2A35_000912"
     CDS             17132..17935
                     /gene="agaC"
                     /locus_tag="M2A35_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCB7197246.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAAIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17925..18716
                     /gene="agaD"
                     /locus_tag="M2A35_000913"
     CDS             17925..18716
                     /gene="agaD"
                     /locus_tag="M2A35_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCB7197247.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18717..19472
                     /locus_tag="M2A35_000914"
     CDS             18717..19472
                     /locus_tag="M2A35_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCB7197248.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQQLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            19872..20456
                     /locus_tag="M2A35_000915"
     CDS             19872..20456
                     /locus_tag="M2A35_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7197249.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            20536..21231
                     /locus_tag="M2A35_000916"
     CDS             20536..21231
                     /locus_tag="M2A35_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7197250.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            21261..23777
                     /locus_tag="M2A35_000917"
     CDS             21261..23777
                     /locus_tag="M2A35_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7197251.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSCAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            23788..24879
                     /locus_tag="M2A35_000918"
     CDS             23788..24879
                     /locus_tag="M2A35_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7197252.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(24922..25782)
                     /gene="rsmI"
                     /locus_tag="M2A35_000919"
     CDS             complement(24922..25782)
                     /gene="rsmI"
                     /locus_tag="M2A35_000919"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCB7197253.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            25846..27834
                     /gene="lpoA"
                     /locus_tag="M2A35_000920"
     CDS             25846..27834
                     /gene="lpoA"
                     /locus_tag="M2A35_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCB7197254.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTTQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFR
                     LEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQV
                     QGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            27792..28187
                     /locus_tag="M2A35_000921"
     CDS             27792..28187
                     /locus_tag="M2A35_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCB7197255.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            28207..28797
                     /gene="diaA"
                     /locus_tag="M2A35_000922"
     CDS             28207..28797
                     /gene="diaA"
                     /locus_tag="M2A35_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCB7197256.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            28807..29382
                     /gene="yraP"
                     /locus_tag="M2A35_000923"
     CDS             28807..29382
                     /gene="yraP"
                     /locus_tag="M2A35_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCB7197257.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQSIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(29496..30536)
                     /locus_tag="M2A35_000924"
     CDS             complement(29496..30536)
                     /locus_tag="M2A35_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCB7197258.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(30609..31244)
                     /locus_tag="M2A35_000925"
     CDS             complement(30609..31244)
                     /locus_tag="M2A35_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCB7197259.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNRPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            31372..31890
                     /gene="yhbO"
                     /locus_tag="M2A35_000926"
     CDS             31372..31890
                     /gene="yhbO"
                     /locus_tag="M2A35_000926"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCB7197260.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(31870..32313)
                     /locus_tag="M2A35_000927"
     CDS             complement(31870..32313)
                     /locus_tag="M2A35_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCB7197261.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            32364..32666
                     /gene="yhbQ"
                     /locus_tag="M2A35_000928"
     CDS             32364..32666
                     /gene="yhbQ"
                     /locus_tag="M2A35_000928"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCB7197262.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(32653..33156)
                     /locus_tag="M2A35_000929"
     CDS             complement(32653..33156)
                     /locus_tag="M2A35_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7197263.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(33150..33674)
                     /locus_tag="M2A35_000930"
     CDS             complement(33150..33674)
                     /locus_tag="M2A35_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCB7197264.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSAVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            33883..34878
                     /locus_tag="M2A35_000931"
     CDS             33883..34878
                     /locus_tag="M2A35_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCB7197265.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            34887..35765
                     /locus_tag="M2A35_000932"
     CDS             34887..35765
                     /locus_tag="M2A35_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCB7197266.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            35971..36978
                     /locus_tag="M2A35_000933"
     CDS             35971..36978
                     /locus_tag="M2A35_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCB7197267.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(37096..38340)
                     /gene="mtr"
                     /locus_tag="M2A35_000934"
     CDS             complement(37096..38340)
                     /gene="mtr"
                     /locus_tag="M2A35_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCB7197268.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(38494..40383)
                     /gene="deaD"
                     /locus_tag="M2A35_000935"
     CDS             complement(38494..40383)
                     /gene="deaD"
                     /locus_tag="M2A35_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCB7197269.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(40376..40456)
                     /gene="yrbN"
                     /locus_tag="M2A35_000936"
     CDS             complement(40376..40456)
                     /gene="yrbN"
                     /locus_tag="M2A35_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCB7197270.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(40563..41447)
                     /gene="nlpI"
                     /locus_tag="M2A35_000937"
     CDS             complement(40563..41447)
                     /gene="nlpI"
                     /locus_tag="M2A35_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCB7197271.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(41556..43691)
                     /gene="pnp"
                     /locus_tag="M2A35_000938"
     CDS             complement(41556..43691)
                     /gene="pnp"
                     /locus_tag="M2A35_000938"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCB7197272.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(43938..44207)
                     /gene="rpsO"
                     /locus_tag="M2A35_000939"
     CDS             complement(43938..44207)
                     /gene="rpsO"
                     /locus_tag="M2A35_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCB7197273.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(44356..45300)
                     /gene="truB"
                     /locus_tag="M2A35_000940"
     CDS             complement(44356..45300)
                     /gene="truB"
                     /locus_tag="M2A35_000940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCB7197274.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(45300..45701)
                     /gene="rbfA"
                     /locus_tag="M2A35_000941"
     CDS             complement(45300..45701)
                     /gene="rbfA"
                     /locus_tag="M2A35_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCB7197275.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(45865..48537)
                     /gene="infB"
                     /locus_tag="M2A35_000942"
     CDS             complement(45865..48537)
                     /gene="infB"
                     /locus_tag="M2A35_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCB7197276.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(48562..50049)
                     /gene="nusA"
                     /locus_tag="M2A35_000943"
     CDS             complement(48562..50049)
                     /gene="nusA"
                     /locus_tag="M2A35_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCB7197277.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(50077..50529)
                     /gene="rimP"
                     /locus_tag="M2A35_000944"
     CDS             complement(50077..50529)
                     /gene="rimP"
                     /locus_tag="M2A35_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCB7197278.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(50736..50812)
                     /locus_tag="M2A35_000945"
     tRNA            complement(50736..50812)
                     /locus_tag="M2A35_000945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50776..50778),aa:Met,seq:cat)
     gene            51160..52503
                     /gene="argG"
                     /locus_tag="M2A35_000946"
     CDS             51160..52503
                     /gene="argG"
                     /locus_tag="M2A35_000946"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCB7197279.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(52511..54136)
                     /locus_tag="M2A35_000947"
     CDS             complement(52511..54136)
                     /locus_tag="M2A35_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCB7197280.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(54593..54679)
                     /locus_tag="M2A35_000948"
     tRNA            complement(54593..54679)
                     /locus_tag="M2A35_000948"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54643..54645),aa:Leu,seq:gag)
     gene            complement(54694..55026)
                     /gene="secG"
                     /locus_tag="M2A35_000949"
     CDS             complement(54694..55026)
                     /gene="secG"
                     /locus_tag="M2A35_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCB7197281.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPTAPA
                     KPTSDIPN"
     gene            complement(55254..56591)
                     /gene="glmM"
                     /locus_tag="M2A35_000950"
     CDS             complement(55254..56591)
                     /gene="glmM"
                     /locus_tag="M2A35_000950"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCB7197282.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(56584..57432)
                     /gene="folP"
                     /locus_tag="M2A35_000951"
     CDS             complement(56584..57432)
                     /gene="folP"
                     /locus_tag="M2A35_000951"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCB7197283.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVVPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(57522..59456)
                     /gene="ftsH"
                     /locus_tag="M2A35_000952"
     CDS             complement(57522..59456)
                     /gene="ftsH"
                     /locus_tag="M2A35_000952"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCB7197284.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(59556..60185)
                     /gene="rlmE"
                     /locus_tag="M2A35_000953"
     CDS             complement(59556..60185)
                     /gene="rlmE"
                     /locus_tag="M2A35_000953"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCB7197285.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            60311..60604
                     /gene="yhbY"
                     /locus_tag="M2A35_000954"
     CDS             60311..60604
                     /gene="yhbY"
                     /locus_tag="M2A35_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCB7197286.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(60760..61236)
                     /gene="greA"
                     /locus_tag="M2A35_000955"
     CDS             complement(60760..61236)
                     /gene="greA"
                     /locus_tag="M2A35_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCB7197287.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            61484..62917
                     /gene="dacB"
                     /locus_tag="M2A35_000956"
     CDS             61484..62917
                     /gene="dacB"
                     /locus_tag="M2A35_000956"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCB7197288.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(62957..64129)
                     /gene="cgtA"
                     /locus_tag="M2A35_000957"
     CDS             complement(62957..64129)
                     /gene="cgtA"
                     /locus_tag="M2A35_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCB7197289.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(64145..65110)
                     /locus_tag="M2A35_000958"
     CDS             complement(64145..65110)
                     /locus_tag="M2A35_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCB7197290.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(65237..65494)
                     /gene="rpmA"
                     /locus_tag="M2A35_000959"
     CDS             complement(65237..65494)
                     /gene="rpmA"
                     /locus_tag="M2A35_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCB7197291.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(65515..65826)
                     /gene="rplU"
                     /locus_tag="M2A35_000960"
     CDS             complement(65515..65826)
                     /gene="rplU"
                     /locus_tag="M2A35_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCB7197292.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            66085..67056
                     /gene="ispB"
                     /locus_tag="M2A35_000961"
     CDS             66085..67056
                     /gene="ispB"
                     /locus_tag="M2A35_000961"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCB7197293.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVQEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            67284..67562
                     /gene="sfsB"
                     /locus_tag="M2A35_000962"
     CDS             67284..67562
                     /gene="sfsB"
                     /locus_tag="M2A35_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCB7197294.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(67610..68869)
                     /gene="murA"
                     /locus_tag="M2A35_000963"
     CDS             complement(67610..68869)
                     /gene="murA"
                     /locus_tag="M2A35_000963"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCB7197295.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATMKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(68924..69178)
                     /gene="ibaG"
                     /locus_tag="M2A35_000964"
     CDS             complement(68924..69178)
                     /gene="ibaG"
                     /locus_tag="M2A35_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCB7197296.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(69338..69631)
                     /gene="mlaB"
                     /locus_tag="M2A35_000965"
     CDS             complement(69338..69631)
                     /gene="mlaB"
                     /locus_tag="M2A35_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCB7197297.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(69631..70266)
                     /gene="mlaC"
                     /locus_tag="M2A35_000966"
     CDS             complement(69631..70266)
                     /gene="mlaC"
                     /locus_tag="M2A35_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCB7197298.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(70285..70836)
                     /gene="mlaD"
                     /locus_tag="M2A35_000967"
     CDS             complement(70285..70836)
                     /gene="mlaD"
                     /locus_tag="M2A35_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCB7197299.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(70841..71623)
                     /gene="mlaE"
                     /locus_tag="M2A35_000968"
     CDS             complement(70841..71623)
                     /gene="mlaE"
                     /locus_tag="M2A35_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCB7197300.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(71631..72440)
                     /gene="mlaF"
                     /locus_tag="M2A35_000969"
     CDS             complement(71631..72440)
                     /gene="mlaF"
                     /locus_tag="M2A35_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCB7197301.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            72650..73627
                     /locus_tag="M2A35_000970"
     CDS             72650..73627
                     /locus_tag="M2A35_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCB7197302.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            73641..74627
                     /gene="kdsD"
                     /locus_tag="M2A35_000971"
     CDS             73641..74627
                     /gene="kdsD"
                     /locus_tag="M2A35_000971"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCB7197303.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            74648..75214
                     /gene="kdsC"
                     /locus_tag="M2A35_000972"
     CDS             74648..75214
                     /gene="kdsC"
                     /locus_tag="M2A35_000972"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCB7197304.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            75211..75786
                     /gene="lptC"
                     /locus_tag="M2A35_000973"
     CDS             75211..75786
                     /gene="lptC"
                     /locus_tag="M2A35_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCB7197305.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            75755..76312
                     /gene="lptA"
                     /locus_tag="M2A35_000974"
     CDS             75755..76312
                     /gene="lptA"
                     /locus_tag="M2A35_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCB7197306.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            76319..77044
                     /gene="lptB"
                     /locus_tag="M2A35_000975"
     CDS             76319..77044
                     /gene="lptB"
                     /locus_tag="M2A35_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCB7197307.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            77092..78525
                     /gene="rpoN"
                     /locus_tag="M2A35_000976"
     CDS             77092..78525
                     /gene="rpoN"
                     /locus_tag="M2A35_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCB7197308.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            78548..78835
                     /gene="hpf"
                     /locus_tag="M2A35_000977"
     CDS             78548..78835
                     /gene="hpf"
                     /locus_tag="M2A35_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCB7197309.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            78953..79444
                     /gene="ptsN"
                     /locus_tag="M2A35_000978"
     CDS             78953..79444
                     /gene="ptsN"
                     /locus_tag="M2A35_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCB7197310.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            79490..80344
                     /gene="rapZ"
                     /locus_tag="M2A35_000979"
     CDS             79490..80344
                     /gene="rapZ"
                     /locus_tag="M2A35_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCB7197311.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            80341..80613
                     /gene="npr"
                     /locus_tag="M2A35_000980"
     CDS             80341..80613
                     /gene="npr"
                     /locus_tag="M2A35_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCB7197312.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            80827..>81103
                     /gene="yrbL"
                     /locus_tag="M2A35_000981"
     CDS             80827..>81103
                     /gene="yrbL"
                     /locus_tag="M2A35_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbL"
                     /protein_id="HCB7197313.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSI"
BASE COUNT        20047 a        21577 c        20057 g        19422 t
ORIGIN      
        1 catctctgca atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgccgc
       61 atccggcatg aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta
      121 catcaggtga aactgaccga taagccgctt tcttttgggt atagcgtcgt ggacagtcat
      181 tcatctttct gcccctccaa aagcaaaaac ccgccggttt acgctggcgg gttttcgtta
      241 aatcaggtga aactgaccga taagccgctt tcttttgggt atagtgtcgt ggacagtcat
      301 tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg
      361 tgaaactgac cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg
      421 ctcactggct caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt
      481 tggccttgct ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct
      541 cttaccgcac cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc
      601 tctgttgcac tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag
      661 cccggacttt cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact
      721 gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc
      781 gcattccaat cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag
      841 ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcga
      901 tgccatgctg atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg
      961 gtttatggta cttcttcgcc acgtttgcaa cgccaatcgg taccttgccg tgaatactct
     1021 ggctgtcaat acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcctcca
     1081 gattcagcgc cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca
     1141 tcagcgccgc acccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat
     1201 gcagcgcttt tttaatgacc tcggcatagt gagagaggtt attgtccagc tcaacaatca
     1261 tcgcttcact ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca
     1321 gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc
     1381 cggaaatatc aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc
     1441 cgttggcgtc gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg
     1501 tagcgctacc gccaatgccg ataataatgt ttgtcgcgcc gctctccagc gcctgcagga
     1561 ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca
     1621 gctccagccc actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc
     1681 cccaactggc attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac
     1741 cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaatag
     1801 aaacgtactg tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct
     1861 cgctggcaga taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc
     1921 ctgttaccac atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat
     1981 tgcaatacgc catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt
     2041 cgtagtagca cgccagggcg ctatgatccg ccgttcctaa accatctgct cgcagtgcct
     2101 gcatcatctc cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat
     2161 ccagcgcatt tgccagatcc ttaatatgca gatcaatacg gaagcccggc ttgaagttgc
     2221 ggtccatcac catcggcgct ttggcatcca gcacggtact gcccgccagt ccaccgcgaa
     2281 ttgcctgata caccaggtcc gggttaacgc ccgctttggt tgccagcgtt agcgcttctg
     2341 acatcgcggc aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg
     2401 caccgatttc cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt
     2461 tgtcgaaaat agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg
     2521 gttcaccgcc gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc
     2581 tgatttcacg gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct
     2641 tcgcgccttc aatgatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg
     2701 gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt
     2761 ctgcacctgc agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg
     2821 agtaacctgc tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa
     2881 aaccaacttt catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa
     2941 ggtatcagcc agtttctgag tggcagagcg gaagacgcca agatcgctgc cgacagccac
     3001 aaacgtcgcg ccccattcca gataacgacg cgcatcggct tcgaccggcg cgagaatacc
     3061 gctgggtttg ccgtgcgcac tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc
     3121 cgggtgcgag gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa
     3181 gataccgtct acaccttcgg tagcggcaat ggcatcgacg ttatctacgc cctgctgact
     3241 ttctatctgg accagaatgg tgatgttctt gttcgactga gcgaaataat ccgccacggt
     3301 gccaaacata ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg
     3361 ggttgatgcc accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt
     3421 atagaaaccg atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac
     3481 tactggcgcg ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggagatatc
     3541 gtttggcgca tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc
     3601 agtgctaatc gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag
     3661 tgcggctttg aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt
     3721 ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag
     3781 cgctgaacat cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccaggta
     3841 gccaatcacc agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc
     3901 acagaggcca acaatctctt tcggcgcggt gtcagaaatc accggccagc ccagagcacc
     3961 aaatcctttg ccaaaaaaag ccagcgccat tagcatgacc accagcgtgg tgttgttggt
     4021 gtagttacat aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg
     4081 tgccagggtc agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac
     4141 gccgcccgca aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga
     4201 catgcctttt tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt
     4261 gttgataaaa tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt
     4321 gatgtaatgc agtttgggac cgcttgctgc cgcactgccc ggctttttgt ggtccatatc
     4381 gaccaccgcg ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc
     4441 tgtcgggtta tgaatcaatt tgatccacag cgccgtcagc acaaaaccaa tcacccccat
     4501 aacggtaaag acgtgctccc agacccaggc gaaagtcagc cagccaagca gaggcgaaaa
     4561 gagcgccagc gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt
     4621 cgtcgggaac caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa
     4681 gccgagcata aagcgcataa agaacatgga gatccctgcc caggccagcg ggaacatatc
     4741 aacaaagcct tgcaggaaag tgaatagcga ccagaaaaag aggctatagg tataaacttt
     4801 tttcgagcca aacttatcaa gcagccagcc gccggggatt tgcatcagca agtaggccca
     4861 gccaaaagca gaaaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac
     4921 ttcggtacca gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa
     4981 aataatcagt aatattaaat agcgggtatg cacgcctttc tttttttcgt caacggtgtc
     5041 cagaatcatt ttatttacct cgggtactta tgctgatttt tattattatg gggaaggtgt
     5101 tatttatgag tttcatttat gccgtaacgg caatgaactc gggaattagt ataagcagcg
     5161 cgagaataat aatcattgtg caaatgctaa tttaattaat gctatttaaa tattattttg
     5221 agcatatgca cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg
     5281 cactgcaatc cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt
     5341 cttatttcac ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac
     5401 ccctggctgg agaatatagt acaatggcca acatcgaaat cagacaagaa acgccaactg
     5461 cgttttatat aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc
     5521 tgaaagcagg aacgcgtttt ccggatggac tggaattaat tgaacatatt ccccaggggc
     5581 ataaagtcgc attgctggac attccggcta atggtgaaat tattcgttat ggtgaagtga
     5641 ttggttacgc cgtgcgtgca atcccacgcg gaagctggat cgacgaatca atggttgtac
     5701 taccggaagc gccgccgtta cacacgctgc cactggcaac caaagtcccg gcccccttac
     5761 cgccgctgga aggatacacc tttgagggct atcgcaatgc cgatggcagc gtgggcacca
     5821 aaaacctgct cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactatgtag
     5881 taaaaatcat tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc gtggtggggc
     5941 tgaatcattt gtacggctgt ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc
     6001 gtaccatcca caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggtc
     6061 tgggttgtga aaagttgcag cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta
     6121 ttccagtaga aagcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcagt
     6181 ccatggtcga ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc
     6241 agcgagaaac ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg
     6301 ccttttctgg cgtaacggca aacccggcgg ttggctatgc gtctgatcta ctggtgcgct
     6361 gcggcgcaac ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc catctgctga
     6421 caccacgcgc agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg
     6481 ataactatct caatatggga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga
     6541 aaggcggtct ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa
     6601 gcgcaattgt tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg
     6661 ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg
     6721 tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta
     6781 aaatggcgac ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaacgcgg
     6841 gcactatcgc caccggcgaa gaaaccattg aagaggtggg ctggaagttg ttccacttta
     6901 ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga tcaatggggg ctgcataacc
     6961 agctggcagt gtttaacccg gcaccggtga cctgatttct tttggaacgt ccctcgcaaa
     7021 acatggcctt agtgccatgt ttttattgtt taaagccccc acgtccatta ataatgcatt
     7081 tgcattacct tagttcaagc ttataatttg agcagaaaac aggctgtaaa aggacagtga
     7141 atcatgcccg ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata
     7201 cgcggacaaa caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggccag
     7261 gacagcattc attacgaaat tctgcctggt gggcaagtct ttatgtgccg actgggagat
     7321 gaacaggagg atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac
     7381 aacccgcaaa aaactcgtcc attcaacatt caacaaggaa agaaacttgt cgctggcatg
     7441 gacgtcaaca ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta
     7501 atggttgggc gctatatgct catccctgtt ttcaggaaac ctacgacgct ttagttgccg
     7561 aagtcgagac attaaaggga aaagatcctg aaaattatca gagaaaagcc gccacaaagt
     7621 tattggcggt agtccataaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg
     7681 cattccgtca tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat
     7741 ttggtgctgg tcgttatcgt ctcttctttc gttatagtga aaaagagaaa gtcatcattc
     7801 tgggatggat gaacgatgaa aacactctgc gcacctacgg taaaaaaaca gatgcctata
     7861 ccgtattcag caaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc
     7921 aagagacaga agaaaaccat tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga
     7981 ccagcgggca ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt
     8041 ccttccagac tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta
     8101 tcaatgatct tatgtaaact ggaacgattg aatttactgg aatcggtgac cacgatgatc
     8161 cgttccgcaa cttcgcacat ccgacggttt aaacgggctt catcttcatt atgtgtgctg
     8221 acgccgcgct ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt
     8281 tgcagcgatt gctcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg
     8341 ccggtcatca gcagctcaac gccttccgct tccagcaacg cattagccac gttcataccg
     8401 ttggtcatcg caattacgtc agtgtgctta cgcatcagac tagcaatctc aaaagtggta
     8461 gtcccggaat cgaggatcac ccgatgacca ggctgaatca actcaacggc agctttcgca
     8521 acgctgcgtt tcatcgcggt gttcagcgcg cttttatctt ccactgatgg ctcgactgac
     8581 ggcgtcgtgc tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt
     8641 tccagaaacg ccagatcatt gcggatcgtc acagtagata cgccatacaa tgccgacaga
     8701 tcgttaacct gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg
     8761 ctggtgccag tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc
     8821 gtaaaacttt cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag
     8881 cccaccttgc ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt
     8941 gtgaatcaga tcagaaaact attatctttc gttttatttt tatctcacca tgacgcagta
     9001 tcaactgaaa caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa
     9061 acatctgaca gaaatggtga gacagcacaa agcgggcaaa acaaatggaa tttatgccgt
     9121 ttgttccgca catccgctgg tgctggaggc tgcaatccgc tacaccagtg caaaccaaac
     9181 gccgttactg attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat
     9241 gacgcccgcc gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca
     9301 ggatgcgttg attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccggc
     9361 cgctcaggca atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa
     9421 aaaaatccac cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga
     9481 catcgtggct gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca
     9541 ctttggcgaa gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc
     9601 gcatgaaacc ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccatgctgga
     9661 agcccatcgt cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc
     9721 cctggtggtt caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc
     9781 caaagcgagc gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca
     9841 ctctaccgat tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat
     9901 tctgaaagtt ggcccagcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc
     9961 gattgaagaa gaactggtgc cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga
    10021 cgtgacgctc gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg
    10081 tcgtctggcg cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca
    10141 gattgatgac gctttcgctc atctggtacg taatctggcg gattcaccaa ttccgctgcc
    10201 gctgatcagc cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc
    10261 aacgccacgg gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc
    10321 ctgtgaaggc caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc
    10381 ggattgatga acgcttgatt cacggtcagg tcggcgttca atgggtcgga tttgcggggg
    10441 caaatctggt gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga
    10501 tggaaatggt actggcagaa gggagcgccg tacgtttctg gacgctgcaa aaagttatcg
    10561 acaacattca tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg
    10621 atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc
    10681 actacgccaa tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg
    10741 cagcatttaa cgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag
    10801 agcctgctgt ggacctcttt aaattacttt gagggattca tcatggaaat cagcctgttg
    10861 caggcatttg cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggccta
    10921 acacatatgc accgcccggt ggtcctcggc ccgttggtcg ggctggtact tggcgatctg
    10981 cataccggaa ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg
    11041 gcgggcgcac agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt
    11101 actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta
    11161 cagatgggga ttaccttcct gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg
    11221 atggcggaga acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca
    11281 ctcggcactt tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca
    11341 aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc
    11401 atcatgccag caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc
    11461 ccctacttca tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt
    11521 gctgccgctg cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact
    11581 caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa
    11641 cgtcagtgaa aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca
    11701 gagcattggc gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct
    11761 gttacaagcg tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct
    11821 gcctgctctg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca
    11881 tatgggtttc ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc
    11941 gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc
    12001 gccgctcggc gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg
    12061 gataggcgca agccttgcgc tacaaggctc tattcttggc gcagtcgtat ttattgtgct
    12121 gttcaacgtg gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg
    12181 cgtggcggcg attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat
    12241 cgttgggatg acggtaatcg gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct
    12301 ggaaatcaca gcgggcgacg cagtggttaa gttacagact gatgttattg acaaactgat
    12361 gcctgccttc ttaccgctgg tctacacctt gaccatgttc tggctggtac gccgcggctg
    12421 gagtccgctg cgcctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca
    12481 tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg
    12541 tttgccagtg gcatggaaaa agcaatgaag caaatcctcg gcgagcaatc gcagtttatt
    12601 gccatcgatt ttcccgaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc
    12661 atcgcccaac tggattgtga agacggcatt gtttttctga ctgatttgct gggcggcacg
    12721 ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc
    12781 accaatttac aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagag
    12841 ttccgtgtgc aggcgctgga gtgtggacat cgtgggctga ccagtctggt agacgagtta
    12901 ggccgctgcc atgaagaatg tccggtcgag gaaggaatat gacacccgtt ctgcgcgcca
    12961 gaaggctgct gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg
    13021 tcatcgcagc aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct
    13081 gccctgctta catcgacacc catgtacacg ggggtgcggg cgttgatgta atggatgacg
    13141 cgccggatgt actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt
    13201 taccgaccac cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa cgtattgctc
    13261 aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt
    13321 acttcacgcc gcagaataaa ggcgcgcaac cgccggaatt gtttcgcgag cttgaaattg
    13381 ccgagctgga tcaattgatt gccgtttctc agcacacctt acgcgtggta gcgctggcac
    13441 cggaaaaaga gggggcattg caggccatcc gccatcttaa acagcaaaat gtacgagtga
    13501 tgctggggca tagcgcggcg acctggcaac aaactcgcgc cgcgtttgat gctggtgccg
    13561 acggcctggt gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg
    13621 ttggcgcggg attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg
    13681 tgcatccggc ggcaatgtcg ctgtgttgtt gctgtgcgaa agagagaatc gtactgatca
    13741 ccgacgcgat gcaggcagct gggatgccgg atggtcgcta tacgttatgt ggtgaagaag
    13801 tgcagatgca cggtggcgtt gtccgtaccg cgtctggtgg gctggcgggc agtacgctgt
    13861 ctgttgatgc ggcagtgcgc aatatggtcg agttgacggg cgtaacgcct gcggaagcca
    13921 tccatatggc gtcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc
    13981 ttaaaccggg caaacgcgcc agcgtcgttg cgctggatag cgggctacat gtgcaacaaa
    14041 tctggattca gggtcaatta gcttcgtttt gatggtttgc tccattattg ggccttcact
    14101 tcccccgtaa ggcctttctt tttctttctt tttgatctgt gcagcggtgt cggatgcgac
    14161 gctaacgcgt cttatgcgac ctacggtacg ccgcatccgg caatcggtga acatagcgac
    14221 gtcattacgc aattctttaa tcttcccttg tcttttcttt caacgatcac agatttcgtt
    14281 ttatttcctt tttcttcatt gaactttcgg tttcttttct atagatttta atcaacgaaa
    14341 gacatcacca agtgaaatta aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt
    14401 tcatcagact aaggattgag ttatgccaga aaattacacc cctgctgccg ccgcaaccgg
    14461 tacatggact gaagaagaga tccgccatca gcctcgcgca tggatccgtt cactcaccaa
    14521 catcgacgcg ctacgttccg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa
    14581 tctgcggatc atcctgaccg gagccggaac ctcggcattt atcggtgaca tcatcgcgcc
    14641 gtggctcgcc agccataccg gtaaaaactt cagcgccgta ccgaccaccg atctggtcac
    14701 taatccgatg gactacctga acccagccca tccgctgctg ttgatctcct tcggtcgatc
    14761 cggcaacagc ccggaaagcg tcgctgccgt ggaactggca aatcaatttg taccagaatg
    14821 ctatcacctg ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag
    14881 cgataacgcg tttgccctgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac
    14941 cagcagcatt accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa
    15001 cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga
    15061 tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg
    15121 tggcttacag ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga cggcgggtaa
    15181 actggcggcc ttttatgatt ctccaaccgg attccgtcat ggaccaaaat cgctggtcga
    15241 taacgaaaca ctggtggtgg tatttgtctc cagccaccct tacacccgtc agtatgatct
    15301 tgatctgctg gctgaactcc gccgtgacaa ccaggcaatg cgcgtaatcg ccatcgccgc
    15361 ggaaagcacc gacatcgtcg ctgccggtcc acatattatc ctgccgccgt cacgtcactt
    15421 tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca
    15481 gtcgctgcac atgggcaata cgccggatac cccatcagcc agtggcaccg ttaaccgcgt
    15541 ggtgcaaggc gtaatcattc atccgtggca ggcataagag gatcgcatta tgagcattat
    15601 ctccactaaa tatctgttac aggacgccca ggccaatggc tacgcggtgc ctgcttttaa
    15661 cattcataac gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc
    15721 ggtgatcctc gccggaacgc cggggacctt taaacatatc gcgctggaag agatctacgc
    15781 cctgtgcagc gcctattcca caacctacaa catgccactg gcgctgcatc tcgaccacca
    15841 cgaatcgctg gatgatattc gccgtaaagt ccacgcaggt gtgcgcagtg caatgatcga
    15901 cggcagccac ttcccgtttg ccgagaacgt gaagctggtg aaatcggttg ttgacttctg
    15961 ccactcacag gattgcagcg tggaagcaga actgggccgc ctgggcggtg ttgaagatga
    16021 catgagcgtt gacgccgaaa gtgcattcct gaccgatcca caagaagcta aacgctttgt
    16081 cgaactgact ggcgtcgaca gcctggcggt agcgattggt acggcgcacg gcttatacag
    16141 caaaacgccg aagattgatt tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc
    16201 tctggtgcta catggtgcca gcgatgttcc ggatgaattt gtccgtcgca ctattgaact
    16261 tggcgtcaca aaagtgaacg ttgccacaga attaaaaata gccttcgctg gcgcggttaa
    16321 agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat
    16381 ggatgcgatg aaagaagttg tcagaaataa aattaatgtc tgtggttcag cgaatcgaat
    16441 ttcagcataa tcattagttt tttatgattt atcccaatgt acttcccgat taatatcggg
    16501 gagtgcctta atggaaaagg agatgactaa accttaataa atacatcagt acaatatcgc
    16561 aacaataata tattaaaaaa aatcatatta ttcaacttta tggtgaggat tacacaatga
    16621 gcagtccaaa tattctctta acccgtattg ataaccgtct ggttcacggt caggttggcg
    16681 tgacctggac atccaccatc ggtgcgaatc tgctggtagt cgtggatgat gttgtcgcta
    16741 acgatgatat tcaacagaaa ttaatgggta ttaccgcaga aacctacggc tttggcattc
    16801 gtttctttac tatcgaaaaa accattaacg tcatcggcaa agctgcaccg catcagaaga
    16861 tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc
    16921 tgaaagatat caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta
    16981 aagtttatgt cgatgaccag gatctcgcgg acttacgttt tattaaacaa cgtggcgtga
    17041 atgttttcat tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa
    17101 atcgccttaa tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt
    17161 atccctggcg gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt
    17221 attccgcccg ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg
    17281 cttaattacc ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt
    17341 tcagccgccc aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg
    17401 cgttgatgcc aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt
    17461 cattctgttc ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa
    17521 agaggcggat acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc
    17581 agcgtatgcg gcgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct
    17641 ggtgaaagcg atgcccgcct ggttgaccca cggctttgaa gtggctggcg gtattctgcc
    17701 tgccgttggt tttggcttgc tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct
    17761 gatcgctggc ttcctgtttg tttgctacat ccaggtcagc aacctgttgc cggttgccgt
    17821 actgggtgca ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca
    17881 accgcagccc gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca
    17941 gtaaaaaaga tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact
    18001 acgaaaggat gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt
    18061 ataaggacga caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata
    18121 cccacccgaa tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag
    18181 aaaaccgcga cactattaaa ggcctcaaag tggcactgtt tggcccaatt gccgggattg
    18241 gcgatgcgat tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg
    18301 ccagccaggg aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt
    18361 tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag
    18421 tgcgagagaa ctcgcagatg attgctcgct cggcaaccat cctcgggatc acggtaatcg
    18481 gcgggctgat cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta
    18541 cccacagcgt cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga
    18601 tggcctacac cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt
    18661 taatcggcgt gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg
    18721 aacgaggcac tgcgtctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc
    18781 ttcagcaagt tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat ttattggccg
    18841 tgatccgtag caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga
    18901 cgtatcatta tctggtagaa aaaatccacc agcagcaggt tgatgtcagc cagctcacct
    18961 tcgtgaagct cgacgaatgg gtggatctgc cattaacgat gcctggtacc tgcgaaactt
    19021 tcctgcaaca gcatatcgtg cagcagctgg ggctacgtga agaccagctc atcagctttc
    19081 gctccgaaga gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag
    19141 gcggtctgga tttatgcgtt ctaggattgg ggaaaaacgg tcatcttggg ctgaacgaac
    19201 cgggagaaag cctacaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac
    19261 atgagatgtt aaaaaccacg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg
    19321 atattctcaa tgcccgcgag gttttgttat tggtgactgg cgaaggaaag caggatgcga
    19381 cagaacgttt tctcacggct aaagtctcca ccgctatccc ggcttcgttt ttatggctgc
    19441 acagtaactt tatttgttta attaatacct aaccagatga aaatttgctc aaaattttta
    19501 aattaactta ttcaacagtc acgcattata ttaaataaca tttgactggg ttgaacataa
    19561 cgccaatagc aaaaggagtt atgttcaaca atttaatttt agatatacat ataacttgtt
    19621 ctattaataa atcacctcgt attctgactc ggcccctggc gcagcaaagt caggcattta
    19681 tactcttttt aacacataga aaattcatat caatataata aaaacataaa atatttgcat
    19741 ataaatataa tcttaaagtt tagtctattt aatgttcaat gaaatatttc tacctgtata
    19801 atctttaaag atgttgaaca tatattcaca ttaaatatga ttatgtactt gttacaagga
    19861 taaggttata aatgaataaa gttacaaaaa cagctattgt gggcttactt gcgcttttcg
    19921 caggtaatgc cgctgcaacc gatggtgaaa tagtttttga tggcgagatt ttaaaatccg
    19981 cttgtgaaat caatgactct gataagaaaa ttgaagttgc tcttggtcac tataatgccg
    20041 aacagtttcg ttctgttggc gatcgtagcc caaaaattcc attcactatt cctttagtta
    20101 actgcccagt gactgggtgg gagcacgata acggcaacgt tgaagcgtct ttccgtttat
    20161 ggctggaaac gcgggataat ggcaccgtcc ctaacttccc taatctggca aaagtgggct
    20221 cttttgctgg cacagcggca acaggtgtgg gtatccgtat tgacgatgcg gaaagcggaa
    20281 accttatgcc actgaatgct atggggaatg ataacacggt ttatcagatc ccagcggact
    20341 ccaatggtat tgtcaatgtt gacctcatcg cttactacgt atcaaccgtt gaagcatcag
    20401 aaatcacccc aggggaagca gacgctgtcg ttaacgtaac gctggattac cgttaatacg
    20461 ttacggcgtt atctgacctt tcagataacg cccttttcct tcctctttct cgttgtatca
    20521 ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga taataacctt gttgtgtagc
    20581 ttctgtatcg gccaggcgct tgcagggggt atcgttttac agcgaacgcg agtgatctat
    20641 gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca aaggcgcaga aacgccttat
    20701 ttactgcaat catgggtaga taatatagat ggtacaagcc gtgccccatt tattataacc
    20761 ccaccgctat ttcgtcttga agctggcgat gactcatcac tgcgaattat taaaacagct
    20821 gataacctgc ctgaaaataa agagtcgctg ttctacatta atgttcgtgc cattccagca
    20881 aagaaaaaat cagatgatgt taatgctaac gagttgacgc tggtatttaa aacacggatc
    20941 aaaatgtttt atcgccccgc acacctgaag ggacgggtaa acgatgcgtg gacatcactg
    21001 gaatttaaac gtagtgacca ttcactcaat atatataacc caactgaata ttacgtcgta
    21061 tttgccggac tggcagtcga taaaaccgat ctcacaagca aaattgaata tatcgcgccc
    21121 ggagaacata aacagttacc acttcctgca tctggcggaa agaacgtgaa atgggctgca
    21181 atcaatgatt atggcggcag ttccgggaca gaaactcgtc cactgcaata acaaatataa
    21241 aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga
    21301 aacagttggc gctcatcatc aaacgctgtt tgccgatggt gctcactggc agcggcatgc
    21361 tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaactacaa
    21421 aaagtggtat gcaaacaact gatctgtcac gtttttcaaa aaaatacgca caactaccag
    21481 gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat
    21541 ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc
    21601 tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca
    21661 actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac
    21721 ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt
    21781 ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata
    21841 accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa aatggtgcta
    21901 attttggccc ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa
    21961 gctggaacac tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc
    22021 ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt actggcgtgc
    22081 aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac
    22141 gcggtatcgc aaacagttgt gcaatcgtga ctatcaggca aaatggttat gtgatctatc
    22201 aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg
    22261 gcgatttaga agtcacgatt gaagaaagtg acggtacgca acgtcgcttt atccagcctt
    22321 attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac
    22381 gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa
    22441 tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt
    22501 atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata
    22561 tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat caatggcgta
    22621 cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgtcagc tactatcgct
    22681 ataccaacga tggctatttt agttttaatg aagccaatac ccgtaattgg aactataaca
    22741 gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat ggggtaagtc
    22801 tgtatgcctc cggttcgcag caagactatt ggggcaataa cgagaaaaac aggaatatct
    22861 ctgttggggt ttccggtcag caatggggaa ttggttacag cctgaattat caatacagtc
    22921 gctacacgga tcaaaataat gaccgcgcac tctctttgaa tctcagtatt ccgttagaac
    22981 gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat cgcccaaccc
    23041 aacatgaaat gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc tatagcctgg
    23101 aacaaagtct ggatgacgat aacaaccata acagtagcct gaacgccagt taccgttcac
    23161 cttatggcac cttcagtgcc ggatacagct acggtaatga cagcagccaa tacaattacg
    23221 gcgttaccgg cggcgtggtt atccatcctc atggcgtgac gctctcgcaa tatctgggca
    23281 acgcttttgc gcttatcgat gctaatgggg cttctggcgt gaggatacaa aactatccgg
    23341 ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact taccaggaaa
    23401 accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgag caaacaacac
    23461 agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt
    23521 atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg
    23581 ccagcaacga tgagacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct
    23641 ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac
    23701 aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca
    23761 cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg
    23821 ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagctga cagcactagc
    23881 ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg
    23941 acaggacggg agtttacctc tgccacgcta agtagcacta actggcaata cgcctgtacc
    24001 tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccctgtact taccaccact
    24061 ggacatcaag caggatatta caaactcaat gatagtctgg atattaaaac cacattacag
    24121 gcgaatgaca ttccaggact cgtgactgac cagaccgttt ctgttaacac ccgattcaca
    24181 cagataaaaa gcaacaccgt atattctgct gcaacccaaa cgggtgtttg ccagggtgac
    24241 acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct gtatgtcacc
    24301 aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat taaaggcgca
    24361 tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt caagttatcg
    24421 attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga tgttattaag
    24481 gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat gccagatggt
    24541 tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa gattaaaaac
    24601 tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa cctggtcgcc
    24661 agacgaagaa gttcagacaa tgcgcccgat gtcggtattc gtattgaaaa tctcggcggc
    24721 ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc cgggcatggc
    24781 accatcaaca tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa
    24841 tttcagggca ccgccaccat taccgtcatc gtgcggtaaa agaagacgtt gcgttttgat
    24901 taacgcaacg cttcggcact cttacccctg ctgctccagc gcatacttat acagcgcatt
    24961 tttcttcacg ccgtgaattt ctgccgccag cgccgccgct tttttcaacg gcagttctgc
    25021 ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg
    25081 accttcgata atcagcacca tttcgccttt gcgacggttt tcatcttctt ttacccacgc
    25141 cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc
    25201 cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag
    25261 acggtgggtg gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc
    25321 atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac ggtcagaggg
    25381 taaacccgcg gcgcttaacg cagtgatagc agcacacggc ccgggtagcg gcaccacgca
    25441 aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa ttagcggcgt
    25501 tccggcatcg gaaaccagcg caatgttctg cccttcttgc agcttcgcca gcagcgtttc
    25561 ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg
    25621 ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa cggcctgtaa
    25681 tacctctaac gcacgctggg tgatatccgc cagattgccg attggcgtcg gtacaatgta
    25741 aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc
    25801 gatttaatat tgagcattgc gtaaaaaaat atcactggat acattatggt accctcaaca
    25861 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
    25921 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac gacgcaggct
    25981 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
    26041 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
    26101 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
    26161 gtagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
    26221 acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
    26281 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctc
    26341 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
    26401 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
    26461 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
    26521 gccgactggc aaaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg
    26581 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg
    26641 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat
    26701 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagctgaa
    26761 caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc ggttagcgat
    26821 ctgaccggtg aacagcctgc ggcacagcca gcgcctgtaa gcaccccggc agcaaccacc
    26881 acagcggtaa gcgcgccagc gaatccatcc gcagaactga aaatctacga tacctcatca
    26941 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt
    27001 ccgttgctga aaaataacgt tgaagagttg cttaagagca acaccccgct gaacgtgctg
    27061 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
    27121 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
    27181 ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaggag
    27241 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
    27301 cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg gtaccccgat tactcccaga
    27361 gcgacaaccg atgaccagtt caccaataat ggcggtcgtg tcgatgcggt gtacattgtg
    27421 gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg ccatgcgtaa cggtagccag
    27481 agcggtgcaa cgctgtacgc cagctcccgc agtgcgcaag ggaccgctgg cccggatttc
    27541 cgactggaga tggaaggctt gcagtacagc gaaatcccga tgctggcagg cggtaatcta
    27601 ccgttaatgc agcaggcact cagcgcggtg aataacgatt attcactggc tcgcatgtat
    27661 gcgatgggcg tcgatgcctg gtcgctggca aatcatttct cacaaatgcg ccaggttcag
    27721 ggttttgaaa tcaacggtaa taccggaagc ctgacggcaa acccggattg cgtgattaac
    27781 aggaagttat catggctaca gtaccaacaa ggtcaggtag tccccgccag ttaaccacca
    27841 aacagaccgg cgatgcgtgg gaagtacaag cgcgtcgctg gctggaaggc aaaggactgc
    27901 gatttgtcgc cgctaacgtg aacgagcgtg gcggcgagat cgatctgata atgcgtgaag
    27961 gccggaccac cgtttttgtc gaggtacgct atcgccgctc tgcgctttat ggcggcgcgg
    28021 cagccagtgt gacccgcagc aaacaacaca aattattaca gactgcccgc ttgtggctcg
    28081 cgcgtcataa tgggagtttt gatactgtgg attgccggtt cgatgtggta gccttcaccg
    28141 ggaatgaggt tgagtggatt aaggatgcct ttaatgacca ctcataatta aggtttaagg
    28201 attagcgtgc aagaaagaat taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg
    28261 gcggcagagg cgcttccgga tgccatctcc cgtgcagcca tgacgctggt tcagtctctg
    28321 ctcaatggca acaaaatcct ctgttgtggt aatggaactt ccgctgccaa tgcacagcat
    28381 tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc tgccattgca
    28441 ctaaatactg ataatgttgt cttaacggcg attgccaacg atcgcttaca tgatgaagtg
    28501 tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg tattgttagc catttccacc
    28561 cgtggcaaca gccgcgatat tgtaaaagcg gtagaagctg ccgttacgcg tgatatgact
    28621 attgtggcat tgaccggcta tgacggcggc gaacttgcag gtttgttagg gccacaagat
    28681 gtggagatcc gcattccttc gcatcgtagt gctcgcattc aggaaatgca tatgctgacg
    28741 gtaaattgcc tgtgcgatct gatcgataac acgcttttcc ctcaccagga tgtttaagga
    28801 gaatacatga aggcattatc gccaatcgca gtccttattt ccgcgctgct gttgcaaggt
    28861 tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta ccaaagccgc aactgaccca
    28921 cgcagtgtcg gcacccaggt ggacgatggt accctggaag tgcgcgtgaa cagcgcattg
    28981 tcgaaagacg aacagattaa gaaagaagcc cgtattaatg tcacggcgta tcagggcaaa
    29041 gtgctgctgg ttgggcagtc accaaacgct gaactttcgg ctcgcgccaa acagattgct
    29101 atgggcgtag acggtgccaa cgaagtgtat aacgagattc gtcagggcca gtcgattggt
    29161 ctgggcgaag cgtctaacga tacgtggatc accaccaaag tgcgttcgca gctcttaacc
    29221 agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg
    29281 atggggctgg tgactgaacg tgaagcgaaa gcggcggcag atattgccag ccgggtgagc
    29341 ggcgtaaagc gggtaaccac agcgtttacg tttattaaat agcagcccct tgtaatgcct
    29401 gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg caaaccgtag gtcggataag
    29461 gcgtttacgc cgcatccgac atgttttccc tcaaatcaga acaacagcgc cagcccgccg
    29521 acaatcacac cagacactgc caccatcgcc cctgtcagcc ataaggcttt cgctgggaac
    29581 gctttgcgca gcataatcag tgacggcaaa ctcaccgccg ggagcgtcat caacaatgcc
    29641 agcgccggag cagttcccat acctgccagc atcatcgttt gtacaatcgg aatttctgct
    29701 gccgtgggaa tgacaaacaa gcatcctgct accgccatcg ccatcaccca catcaggctg
    29761 ttatcgacag caccatcggc atgggggaat aaccagacgc gagcggcacc caacaccagt
    29821 actgcaagga tgtaaaccgg gatcgtactc cagaaaagcg tccatagcgc cctgccccag
    29881 cggctaaaaa aaccgccctg tgcttccggt atgtcaattt cgaccggtgc ctgcgtttgc
    29941 ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca gcaacaccat caccagcccg
    30001 gccaccagac gaatcgccgc aaaaccccag ccgaggacaa accccataaa caccagcgtc
    30061 gccgggttta acaccggatt gcccatccag aatgccagcg caccgcccat cgacacctgt
    30121 tggcgacgca ttcccgccgc gaccggagcc gcacagcagg tacacatcat gcccggcagc
    30181 gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt gcccaagcgt acgcaacaac
    30241 cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga tcacccccag cactgccgct
    30301 ttccataccg cgaggaaata gatcatcgcg taatccaacg ccgcctgcca cggattagca
    30361 tccgcctgcg caaggataga tttaccgata ctgtgggttt cggcagcagt aaacgctttg
    30421 ccgtagtaag gttgccattt cacataccag agaccggcaa tgacaacgag aaagaaaagc
    30481 gctggtttcc accactgaat gggcgttgcc gcctgagatg aagactgacc agtcatagca
    30541 ttccccagga ataagttatg ataattgagc gcgtgaatat tacgctcact atcaattctt
    30601 gggaataatt attcagctct tttgcgtaat tctgaagagc ttaaaatcgt cacaccttca
    30661 tgcccgggcc gcatcgcctc tgccagcatg acccgcgcca cgtctctcgc atcaatggat
    30721 ttccagttac cgggtaataa acggaacagc ggcgcaaaaa gcgtttcgtt catccgctgt
    30781 ttgctacgat cgcccagtaa catcgacggg cgagcaatgg tcaatttcgg cctgttctgg
    30841 gcgattaatg cttcttccat ctcccctttg acgcggttat agaaaaacgg cgagtgggca
    30901 ttggcaccca tagcactgac caccagcata tgctgcgcgc ccagtcgccg cccggttaat
    30961 gcggtatcca ctaccagcgt gtaatcggca tgaataaacg cctctttgct ccccgcttct
    31021 cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg gatcggtgac ctgcgccagc
    31081 gcgtcagtca gttgcggatc atggggatta aacaccccgg gcatatcgcc caacggacgt
    31141 cgcgtcggcg cggctatggc gttaactttc ggttcgttaa tcaacatccg cagcaggtga
    31201 ccgcccacca gccccgttgc gcctgtaatc agtacctgac tcatcttcgc tcctttacag
    31261 aattgtctgc cttgcgctcc acggctcatg caccaggctt aatagaccat gaggtaatta
    31321 tccccgattg tgggaaattc gcctcatcca atgcaacaac gcggaggaag catgagtaag
    31381 aaaattgccg ttttaatcac tgatgaattt gaagattcag aatttacttc acccgcagac
    31441 gagttccgta aagccggaca cgaagtgatt accattgaaa aacaagcggg taaaacggtg
    31501 aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat ccatcgatga agtgacgcca
    31561 gcggagtttg atgccctgct gctaccgggc ggccattcac cggattatct gcgtggcgac
    31621 aaccgttttg tcacctttac ccgtgatttt gtgaatagcg gcaaacctgt tttcgccatc
    31681 tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc gggggcgcaa actgaccgcg
    31741 gttaaaccga tcattattga tgttaaaaac gcgggcgcgg aattttacga tcaggaagtc
    31801 gtggtcgata aagatcagct ggttaccagc cggacaccgg acgatctgcc agcgtttaac
    31861 cgcgaggcgt tacgcctgct cggtgcctga gccgcgtagc caaatcattt ttttaccaaa
    31921 gcccagcgtg ttgtcggtga atttgatttc atcgagccgg atttcccaca ccggggccga
    31981 cagcattctg gcaaccggaa agcgacgatt gtacgccttg cgcgcgaggt cgctttcctc
    32041 accttccagc ctgcggatct cacctttaaa ctgcacgccg cgaattaacg ctaccgtttt
    32101 cggctgaccg tttaccgttc cggcaaccgc agcctgcggc ccactcatct gcgcgtggcg
    32161 cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaca gataaaaagc
    32221 attagcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat gttgttttgc
    32281 caaccaacgg ctgatggtga tgagtgtttc cattgctgtt ctcttttata ctgtgggcct
    32341 gaccttaaca tgcgacgcag acgatgacac cctggtttct ttacttgatc cgtaccgccg
    32401 acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa
    32461 gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg ttttctgcgc
    32521 cagtgggcga tcgttcgctg gcgttacggg cggaatatcg cgttaagcaa ctgaccaaac
    32581 ggcaaaaaga gcgtctggta gcggaaagcg cagggtttgc agagctgtta agcagtctgc
    32641 aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac cagttacgcc
    32701 attcaacgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc ctggccagcg
    32761 gcaacgcagg tcgtgatgtg ccgccagttc aaagccaaaa cggctgtaca gcgccggatc
    32821 gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc cttcatagac
    32881 cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg gtgccatgcc
    32941 gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat agccaatgac
    33001 ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt cacgcagatc
    33061 gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgtagcaggg catcaatacc
    33121 cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga tgtctgtttg
    33181 gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag cagcatcatg
    33241 cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt cacatacagc
    33301 cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt atccggatct
    33361 tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact cacatcagct
    33421 tgcgcgttct ggctgaccac cagtttgcca ttcaccgccg aggtaaacca ttgcaggtca
    33481 atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc gccatcatcc
    33541 agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat
    33601 ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat acgggaacgc
    33661 agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt gccatatcag
    33721 aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta ccaccaatac
    33781 aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat cccagttcgt
    33841 taacattttt gcgttttgat agcgcaacct tcaggaaaaa ttatggagct gctctgccct
    33901 gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc
    33961 gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa
    34021 ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat cgcgattaac
    34081 acttttgcgc atccggacgg ttacgcccgt tggcagcgtg ccgtggatat ggcggcgcag
    34141 ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tagagtacgc cgccgagcgt
    34201 tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt
    34261 aacttttatc atcgccattt tgacgttgct cgcgtggtgc tgccgcgcgt gttgtcgatt
    34321 catcaggtga aacaactggc acgggtcaca cctgtaccac tggaagtctt tgctttcggc
    34381 agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt cgtatctgac gggtgagtcg
    34441 cccaacaccg taggcgcgtg ttctccggcc cgtttcgtgc gctggcaaca aacgccgcag
    34501 gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg cgaaaacgca
    34561 ggttatccga cgctatgtaa agggcgttat ctggtggacg gcgagcgcta tcacgcgctg
    34621 gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc ggcgaatatt
    34681 gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca ggtggcgaaa
    34741 gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcat accgcaaagc
    34801 gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc agaccaccct tggcgcgtat
    34861 caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg ctgtggtact
    34921 ggccaaaaga gacgctggaa gatttttatc agcaggccgc caccagcagc gccgacgtga
    34981 tttatcttgg tgaagcgata tgcagcaagc gccgggcaac caaagttggc gactggctgg
    35041 agatggcaaa atcgctcgcc ggaagtggca agcagattgt gctctccacg ctggcgctgg
    35101 tgcaggcatc ctctgaactg ggcgaactga aacgctatgt tgagaacggt gagtttctga
    35161 ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg
    35221 ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa aatattgctc aaacagggca
    35281 tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat ctgcttaatc
    35341 agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc
    35401 taccgctggc ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg
    35461 agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc
    35521 aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac aacctcggta
    35581 acgagctggc atccatgcag gggctggttg atgtggtccg cctgtcaccg cagggtactg
    35641 acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgcg ccactaccgt
    35701 tgacggcaaa cagcgactgt aacggctact ggcggcggct ggccgggctg gagctgcaag
    35761 cctaagtaaa tatgctcgtc atacttcaag ttgcatgtgc tgcatctgca ttcgttcacc
    35821 ccagtcactt acttatgtaa gctcctgggg attcactctc ttgtcgcctt cctgcaactc
    35881 gaattattta gagcacagct cactttgtta acaactttaa ctactcttta atgcagtatt
    35941 aaagattaat cggtaacaaa gtgagctgtt atgactgata aaaccattgc gttttcgcta
    36001 ctcgatctgg cccccattcc cgaaggttct tcagcgcgag aagcattctc ccactctctc
    36061 gatctcgccc gtctggctga aaagcgcggc tatcatcgct actggctggc agaacaccac
    36121 aatatgactg gcattgccag tgctgccacg tcggtattga ttggctatct ggcggcgaat
    36181 accaccacgc tgcatctggg gtctggcggc gtgatgttgc ctaaccactc accgttggtc
    36241 attgccgaac agttcggcac gctcaataca ctctatccgg ggcgaatcga tttggggctg
    36301 ggtcgtgcgc caggtagtga ccaacggaca atgatggcgc tgcgtcgcca tatgagcggc
    36361 gatattgata atttcccccg cgatgtcgcg gagctggtgg actggtttga cgcccgcgat
    36421 cccaatccgc atgtgcgccc ggtaccaggc tatggcgaga aaatccccgt gtggttgtta
    36481 ggctccagcc tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt cgcgtttgcc
    36541 tcacacttcg cgccggatat gttgttccag gcgctgcatc tttatcgcag caacttcaaa
    36601 ccgtcggcac gactggaaaa accatacgcg atggtgtgca tcaatattat cgccgccgat
    36661 agcaaccgcg acgctgagtt tctgtttacc tcaatgcagc aagcctttgt gaagttgcgc
    36721 cgtggcgaaa ccgggcaact gccgccaccg attcaaaata tggatcagtt ctggtcgccg
    36781 tccgaacagt atggtgtgca gcaggcgctg agtatgtcgt tggtaggtga taaagcgaaa
    36841 gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg ccgatgagat tatggtcaac
    36901 gggcagattt tcgaccacca ggcgcggctg cattcgtttg agctggcgat ggatgttaag
    36961 gaagagttgt tgggatagtg tgtcttaacg cgggaagcct tatccgagct ggcaacgctg
    37021 tcctacgtag acctgataag cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg
    37081 ataaggcacc gctgattact gatacaccgg cagtaaatta aagctcgata aaatatggac
    37141 cagtgcgttg ccaacgccaa acaccagaat cagcgtaatc atcggcttgc cgccccagac
    37201 gcggaatttc gggctgccaa agcgtttacg cgatgcgcgg gctaacagcg ccggaacaat
    37261 tgccgcccag atggtagctg ctaaaccagc ataaccaatg gcgtacagga atccgttcgg
    37321 gaacagtaga ccacccacaa caggcggggc aaaggtcagc aatgccgttt tcaagcgccc
    37381 cagggctgag tcatcgaaac caaacagatc tgccagatag tcaaacaaac ccagcgttac
    37441 gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg accagcagaa ggtccaggct
    37501 acggctgttc agcacgccgc ttaatgcctg taccagcaca tcaatattac cgcccttctc
    37561 tgcaataccg ataaactccg gacgcgggat gttacccatc gtcgccagca accagatggt
    37621 atacagcgcc agtgccatca gcgtaccgta caccaggcat ttcacgatgg ttttcggatc
    37681 tttgccgtaa tacttcatca ggcttggcac gttaccgtga taaccaaacg acgccaggca
    37741 gaacggcaat gtcatcagca gatacggcgc ataagatgca ttgctttctg caacgttgaa
    37801 caatgtcgca ggctgcacat gccccaacag gctgccaaag gtgaggaaga aggtaatgac
    37861 tttcgccccc agcacaatcg ccgtcatgcg gctgacggcc ttagtactca accacaccac
    37921 aaacgctacc agcagtgcaa aaccaaaacc cgccgcccgt gccgggacgt ttagcgacat
    37981 ctctgcgaag gtgtgatgca gaattgaacc actggcagaa atataggcat aggtcaggat
    38041 atagagcaca aaggcaatgg aaataccgtt gaccacgttc cagcctttgc ccagcaaatc
    38101 tttggtgatg gtgtcaaaac tcgaaccgat tcggtaattc aggttagctt ccagaatcat
    38161 caagccggaa tgcagcatac agaaccaggt aaagatcagc gccgccattg accagaaaaa
    38221 ccacgccccg gacatgacca ctggcagaga aaacatccct gcgccaataa tggtgccgcc
    38281 gataatcacc acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat
    38341 gagggcttct ctccagtgaa aaatagtgcg actgcgttgt tatgcattgc actgtaccag
    38401 tacacgagta caaaagacag aaaaaaagcc ccgatggtaa aaatcggggc tgtatatatt
    38461 attttacaga ttgtgttcgc tgttcagcga tgattacgca tcaccaccga aacgacgacg
    38521 accggtagag tcatcacgac gcggagcgcg gccttcacga cgttcgccgc taaaacgacg
    38581 accatcacca cggccacctt cacggcgttc accactgaag ttacgaccgc cttcacgacg
    38641 ttcgccaccg aaaccacgac cgccgccacg acgctcaccg ccagtatgcg gctgtgcatc
    38701 gcccagtaac tgcatgttca tcggcttgtt gagaatgcga gtacgcgtaa agtgttgcag
    38761 cacttcaccc ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat
    38821 gttaccaatg taacggctgc tgatgtcgcc ttcgttagca atcgcaccaa cgatatgacg
    38881 aacttcaaca ccatcatcgc ggcccacttc aatgcggtac agctgcatat cgccaacatc
    38941 acgacgttca cgacgcggac gatcttcacg gtcaccacgc gggccacggt cgttacgatc
    39001 gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg
    39061 cggtacgatc agagtacgtt caccctgtgc cattttcagc agtgccgcag ccagagtttc
    39121 gagatccagc tcttcacctt ctgcagtcgg ctgaattttg ctcagcagtg cgcggtattg
    39181 atccagatcg ctgctttcca gctgctgctg tactttagcg gcgaattttt ccagacggcg
    39241 tttgcccagc agttctgcgt tcggcagttc tacttccgga atagtcagct tcatagtacg
    39301 ttcaatgttg cgcagcagac gacgctcgcg gttctcaacg aacagcagcg cgcggccagc
    39361 acgacccgca cgaccggtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat
    39421 atcgtagtta actaccaggc tgatacgctc aacgtccaag ccacgggctg caacgtcggt
    39481 cgcaatcagg atgtccagac gaccatcttt caggcgttcc agtgtctgtt cacgaagcgc
    39541 ctggttcatg tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gagcttcagc
    39601 cacttccaga gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc
    39661 ttccaggaaa cgtaccagtg cttcgttttt gcgcataccc cagacagtcc agtagctctg
    39721 gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat
    39781 aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg
    39841 atgaccttcc gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag
    39901 catttcgtca gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg
    39961 tttcaggtgg tccagcagac ggcccggagt accgacaacg atctgcggcc cctgacgcag
    40021 ggcgcgtaat tgcacgtcat aacgctggcc gccgtacaga gcaaccacat ttacgccgcg
    40081 catgtgttta gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg
    40141 tgccagcacc agaatctgtg gtgctttcag ctcaggatca agattctgca gcagaggtaa
    40201 agagaatgct gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt
    40261 cagcagatgt ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt
    40321 aagggcttca aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc
    40381 catgtagtac gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt
    40441 gatcagcaat tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac
    40501 aacgccctca cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc
    40561 agctattgct ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc
    40621 aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac
    40681 agtgccgtgg cgctgtccaa atcccccaga cttaggtagt acttacccaa atagaagttg
    40741 gtttcactga gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc
    40801 attaacgttt gttcgctaat gttgcccagg tagaactcga caatgttcca tccccactgt
    40861 tccttatccg atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc
    40921 ttctgctcgg cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa
    40981 aacgccagca gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg
    41041 cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca
    41101 taggcagcat caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca
    41161 ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga
    41221 ccgagactat catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt
    41281 aaagcccgac tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc
    41341 ggttgcaatg gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca
    41401 agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga
    41461 agaccacggt tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa
    41521 aagccccccg ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga
    41581 gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata
    41641 cggccctggc gatcaacttc cagaactttc accggtactt cctgacccat ctgcaggtaa
    41701 tcggtcactt tctcaacgcg tttgtcagcg atttgagaga tgtgtaccag accttcttta
    41761 ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt accagtgtag
    41821 acgcggccca cttcgatttc tgcagtgatc tcttcgatac gacgaatagc gtgtttcgct
    41881 ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc gatggtggtg
    41941 ccagtttctt cggtcagggc acggataaca gaaccgcctt taccgataac gtctttgatc
    42001 ttgtccgggt tgatcttgat ggtatggata cgcggtgcga actcagagat atcgccacgc
    42061 ggcgcgttga tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg
    42121 ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcaat
    42181 gcagagatac cgtcgcggga acccgcaact ttgaagtcca tatcgcccag gtgatcttcg
    42241 tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg
    42301 atacccgcaa cggcagcttt gatcggcaca cctgcgtcca tcagcgccag agacgcgccg
    42361 cacacggaag ccatagaaga ggaaccgttg gattcagtga tttcagacac aacacgtacg
    42421 gtgtacggga atttgtccat atccggcatg actgccagca cgccgcgctt cgccagacga
    42481 ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacggagtac
    42541 ggagggaagt tgtagtggaa caggaaggtg tcggtacgtt cgcccatcag ttcatcaaga
    42601 acctgcgcgt cacgagcagt acccagcgtt gcggtaacca gcgcctgcgt ttcaccacgg
    42661 gtgaacagcg cagaaccgtg agtacgcggc agcacgccag tacgcacatc cagaccacga
    42721 atcatatctt tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg gctacgaaca
    42781 acgtttttct cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct
    42841 tcagcaagca gcgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct
    42901 tgtttgtcgg tgatgcggta agcatcactc agacgagctt cagccagtgc agcaacgcgc
    42961 gcgttcagcg cttcgtttac cggctccggc tgccagtccc aacgtggttt accggcttct
    43021 ttcaccagtt cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaacactact
    43081 gcgcccagca tctggtcttc gctcagcaat tcagcttcag attcaaccat cagtacagcg
    43141 gcttcagtac cagcaacaac cagatccagt ttgctctctt tcagctcgtc ctgagtcggg
    43201 ttcagtacgt actggtcatt gatgtaacct acgcgggcag caccaatcgg accattgaac
    43261 ggaataccag acagagacag tgctgcggaa gcaccaatca tcgcgacgat atccggatta
    43321 acttgcgggt taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc
    43381 gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt
    43441 gggcggcctt cacgacggaa gaagctaccc gggatacgac cagcagcgta agtacgctcc
    43501 tgatagttaa cggtcagtgg gaagaagtcc tgacctggtt tggctttttt ctggccaaca
    43561 acggtaacga ataccgcggt gtcatccatg ctaaccataa cagcggcagt agcctgacga
    43621 gccatcatgc cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc
    43681 ggattaagca atgtaatatc ctttctcttt cttagacagt accttacggc actggtgtta
    43741 atacccgatc ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg
    43801 taaagcctct cattagccgc gcgaacctct gcaacggaag atcattcata gcaacaatac
    43861 attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt
    43921 tctgaaactc gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt
    43981 gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc
    44041 agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg
    44101 atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga
    44161 ccaaactcag aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa
    44221 agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa
    44281 agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg acctgttatc
    44341 gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg caacgcggcc
    44401 ttcatcgtca atttcgccca taccgataaa tttgccgttc tcaccttctg taacgcgaac
    44461 cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga
    44521 agacgttaac ggaagattca ccaccgggta gtccgaagct ggactgtcca ttggcatcag
    44581 taatggatca agtaactccg cagccggaat atcctgctgt tcggcttgtt cgaccagttc
    44641 acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg
    44701 caggtaaata acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa tgatggtgcg
    44761 gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt cgtggcgaat
    44821 aaacagcaat tcataaacgg taatcgggcg cgcttcacgc ggtacttcaa tgccctgacg
    44881 cgcatattcg tacagttttt taccctgata tttcagtgct gaatacatcg aagggatctg
    44941 ttcgatatcg ccacggaaag tatccagcgc cgccgccagt tgctctgcgc taaaggttac
    45001 cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg
    45061 cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc
    45121 cccgaggcaa attggcaaca tgccagttgc cagcgggtcc agcgcaccgg tatgcccggc
    45181 acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcattgctgg acataccctg
    45241 cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat
    45301 tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg
    45361 gtcaccaggt ttgacatgcg catcccttca accagagagt tgtcgtagaa gaaggtcagt
    45421 tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct
    45481 tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc
    45541 acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc
    45601 atgcccaggc gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt
    45661 tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa
    45721 aaaggggctg ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc
    45781 ctgataagac gcgccagcgt cgcatcaggc agtccatgca ggatgcggcg taaacgcctt
    45841 atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatctcga tgatttcgaa
    45901 tacttcgatc acatcgccag tgcggacgtc gttgtagttc ttaacgccga taccacattc
    45961 cataccgtta cggacttcgt taacgtcatc tttgaagcgg cgcagggact ccagctcgcc
    46021 ttcgtagata accacgttgt cacgcagaac gcggatcggg ttgtgacgtt taaccacacc
    46081 ttcggtaacc atacagcctg cgatggcacc aaatttcggt gatttgaaca cgtcacgaac
    46141 ttccgccaga ccgataatct gctgtttcag ttccggagac agcataccgc tcatcgccgc
    46201 tttcacttcg tcaatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc
    46261 ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcgtt
    46321 ggacgccgca gccagggtgg cgtcggtttc ggtgataccc cctacgccag aaccgatgat
    46381 cttcacttta acttcgtcag tagacagttt cagcaaggag tcggagatcg cttccacaga
    46441 accctgtacg tctgccttca ggacgatatt cacttcgtga acttcgcctt cggtcatgtt
    46501 ggcaaacatg ttctcgagtt tagatttttg ctgacgcgcc agtttaactt cgcggaattt
    46561 accctgacga tagagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc
    46621 atcacccgca gccggaacgc cggacaggcc gaggatttcc accggaatgg acggacccgc
    46681 ttccagcact tcctgaccca gttcgttacg catcgcacga acacgaccgt attcgaagcc
    46741 acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg
    46801 accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac
    46861 cgcttttagc tccagaactt ccgcctgcag caggatagcg tccagcagtt catcgatacc
    46921 ggtacccgcc ttcgcagata cgtgtacgaa ctggctttca ccgccccact cttccggcag
    46981 gatgccatac tgggagagtt cgttcttaac acgatccgga tcggcttctg gtttatcgat
    47041 cttgttcact gcaaccacca ccggtacctg cgccgctttc gcgtgctgga ttgcttcgat
    47101 ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg actacgatgt ccgttgcctg
    47161 cgcaccacgt gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat
    47221 catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc
    47281 gccagaggcc actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac
    47341 gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gcagcccccg tgtcacggtc
    47401 gctcattacc gcctcttcca gctcgttttc acgacgcagg ataactttgt ggcccatctc
    47461 ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc
    47521 cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc
    47581 gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag ctggcttctg
    47641 gaagccttgc tgcagggaag aacctttacg ttttccgcct ttaccgccac gtactgctgc
    47701 gcgtgcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc
    47761 tgctttcgcg ttacgaccac ggccacggcc gccttcgact tcacgatcgc tttcgtcttc
    47821 tgcctggcga gcatgttgag aagttgtgac atgataatcg ctggaatctt cagtcggttc
    47881 cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg
    47941 tgcttcttct tcgagtttac gacgcgcttc ttcttcagct ttacgcttga gctctgcagc
    48001 ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt
    48061 gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc
    48121 ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg attcttctgc
    48181 ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg caaggcgttc
    48241 agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga
    48301 tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt
    48361 caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg
    48421 tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag
    48481 gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct
    48541 gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc
    48601 cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat
    48661 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
    48721 atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct
    48781 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
    48841 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag
    48901 ttcttccagc gtcgagaaac cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc
    48961 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag
    49021 gtcgtcaacg gtcattacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt
    49081 ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt
    49141 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
    49201 aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc ccagttcagt
    49261 agaaaccgcc tgaacacgcg cgccacgcat acctacacag gcacctaccg gatcgatacg
    49321 tttatcgttg gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt
    49381 aatttcaatc acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat
    49441 ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata
    49501 gagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg
    49561 caggatcacg gcttcagcat tgttgccgag atccaaagag atgttgtcgc ggtttacttt
    49621 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
    49681 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg
    49741 gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc
    49801 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
    49861 taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc
    49921 gatctcttgt tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
    49981 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
    50041 tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc
    50101 cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat
    50161 ttcaccgtct accgctttga taacgccctg ccatttacga cgattttgta ccgccatacg
    50221 gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg
    50281 gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg gatcttcaac
    50341 atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc
    50401 ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac
    50461 cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa
    50521 tgtggacaag cccaccccca agacataaaa aaagggctta aagcccagtt attctgtagt
    50581 cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac
    50641 tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct
    50701 tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc
    50761 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac
    50821 gtggcaaatt ctactcgttt tgggtaaaag atgcaaatac tgctgggatt tggtgtaccg
    50881 agacgggacg taaaatctgc aggcattata gtgatccatg ccacattttg tcaacgttta
    50941 ttgctaatca cgtgaatgaa tatccaattc actttcattt gttgaatact tttgccttct
    51001 cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga
    51061 taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcggaaat
    51121 ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat
    51181 ctcccggtag gtcaacgtat tggtatcgct ttttctggcg gtctggacac cagtgccgca
    51241 ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa cctgggccag
    51301 ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac
    51361 gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt
    51421 ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc gctgggccgc
    51481 gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa tatctggggt
    51541 gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg tctgctgacc
    51601 aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc
    51661 ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc
    51721 gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcatgaagc gaaggatctg
    51781 gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa attctgggat
    51841 gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg
    51901 gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc
    51961 ggcggtcgtc acggcctggg catgagcgac cagattgaaa accgtatcat cgaagcgaaa
    52021 agccgtggta tttacgaagc tccggggatg gcactgctgc acattgcgta tgaacgtctg
    52081 ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg
    52141 ggccgtctgc tgtatcaggg gcgttggttt gactcccagg cgctgatgct gcgtgactct
    52201 ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct gcgccgtggg
    52261 aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg
    52321 acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca attgaccatg
    52381 cgcaacctgg atatcaccga tacccgcgag aaacttttcg gttatgccaa aactggcctg
    52441 ctctcctcct ctgccacttc aggcgtgccg caggtggaga atctggaaaa taaaggccag
    52501 taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc tctgtaaact
    52561 cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc cgtgagtctc
    52621 ctttatagtg tgcaatcact tcctccagcg tttgcggctg ttccggcttt gttaccccca
    52681 tccacgcatt aataaggtaa ttattgtacg cagtggaaaa gatatcgttt tctgttgtgc
    52741 gatccacgcc atccccaaga acggggctat accagataaa catcgggaca tgatatgcct
    52801 gctggctggc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcgcgttcca
    52861 ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttagtaat tcaaaaacct
    52921 gacctagcaa actgtctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct
    52981 gcggttgaaa cacggcgctg gattgcggat aggcgctaca agccggttca tggcttccat
    53041 ttaaatgaag aacaatcagt ttcttttgct gcgtcttttg ctgtaacgct tgcgataaat
    53101 gcggcaacaa caattcatca aatcctctga catagactgt ttccatggcg cgcatggcga
    53161 tactggtaac tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct
    53221 gaaatcctgc ttgattagcc atattaataa tgttgtcggg gtaattatga atgtcatgac
    53281 tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcgccgc
    53341 ttattgcttg attaaacagt ttgatctgtt ttctttgtgc ttcaacttgc ggtgtcgtag
    53401 agcgtgtata tccatacaaa gacatattgt cgacacgtac agactccccc acaatcaaca
    53461 cgtaggtatc aatacctgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa
    53521 ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa atagttgaga ttaaaaaacg
    53581 gcgtataggt agcaaatcgc gacgctagta tatatggact gaacgctttt ttattttttg
    53641 catctttata agcaaattga caagcggaaa ataaactgcc cgaaatgaca atcagcaata
    53701 atattcctgt cacttttttt gtcggcaagg aaacatcata ttttattatt actgccaaaa
    53761 aaagaaggga taaaaaagca aaagcacata gataagggat atacatcccc agcattttga
    53821 caacttcatc cggatcactc tgcagcacac taatcgcaaa tccatcattg aaagttgtac
    53881 caaaggtaca ccatgaatag aggctaatac tcatgtctgc cgcagtcagt aacacaaatg
    53941 gtgcggcaac aagcaggcga agcggaaggg atgatatacg tttaacaacc aggatggtca
    54001 ggacgaagaa cagcaatcgc tgaaccatat gcgctccgga agaggcgact aagttcgtaa
    54061 taccaaaaac aataacacaa agatacaaca accaatgaga tttaaaactt ctagcaaatt
    54121 tgttgaatac tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat
    54181 gttcaatttt atatcagaca tgtttttcaa ctctgatcca tatcagaccg aaacactgtt
    54241 agttttttct ttttaaatga tgcggccatt gttctcagta attaaataac attatcaatg
    54301 cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg
    54361 tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg
    54421 ccaattatgt cagcagtagg gaaaagttta taactaaggc gtaccggcac catcgtttca
    54481 aagtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca
    54541 gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg
    54601 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
    54661 ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt
    54721 agccggagca gttggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
    54781 gctaccttta ttggttttat tgctattgat gttacccagc accagactga tgatgaagaa
    54841 taacgttgcc agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
    54901 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
    54961 cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc
    55021 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
    55081 gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt
    55141 cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
    55201 tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg
    55261 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
    55321 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcagcaacac gcgtccacgg
    55381 ttgcccagcg cagcttcaac ctctgcggtc acggctttaa ctgactcatg ctcaagtgga
    55441 tcgccgctac ctgcggtgta acgtacgtta accagaatct gcgggaacat tttcatgccg
    55501 ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca
    55561 gcaacgatgc cgtcaccggt agtagtttta tccagtagga tcacatgacc ggaattctct
    55621 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
    55681 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
    55741 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
    55801 tacatgatct gatcgccatc gactttattg ccttcatggt cgaccataat cacgcgatcg
    55861 ccatcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
    55921 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg
    55981 atagcgataa cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca
    56041 ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg
    56101 gctttgcaaa actcgatata gcgacccgcg gcatcaacga tacggctggc tttacccagt
    56161 tctgccgaat caacgcagct gatctccttt tccatttccg cttcgatggc ctcttctacc
    56221 gcatccggca gtttggtgcc gtcgatagag aagaatttaa tcccattatc gtagaacggg
    56281 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
    56341 acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc
    56401 gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc
    56461 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
    56521 tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
    56581 cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc
    56641 ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat
    56701 gaccgcacag gccagactac cgctcaggcg ctcggacggc accacgttca gcagctgccc
    56761 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
    56821 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
    56881 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
    56941 gtagcgattc acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg
    57001 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc
    57061 gccaggttcg gaaagggagc ggatatcatt aataatgtga gcgccaactt tcgctgactc
    57121 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
    57181 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc
    57241 tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcattga tcatcagatt
    57301 cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg
    57361 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
    57421 aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
    57481 ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc
    57541 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
    57601 ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga
    57661 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
    57721 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca
    57781 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
    57841 tcggacatat gtttcgcttt cgctacgcta cggccgagga atacttcacc ttcttcttcc
    57901 gcgtacagca gtggacccaa tttctcagag aagccccact gggtcaccat gttacgtgcc
    57961 agattggtcg caactttaat atcgttggac gcaccggtag aaacatgttc cggcccatag
    58021 atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga
    58081 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
    58141 cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc
    58201 gcatgacccg cttcgtggta agccgttgat tctttctgcg cttccgtcat caccatggag
    58261 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca
    58321 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
    58381 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggggccaat
    58441 ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagccg
    58501 accacaacct gacggtcgaa acggccagga cgcagcaggg ccgggtcgag aacgtccgga
    58561 cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc
    58621 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc
    58681 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc
    58741 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
    58801 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac
    58861 gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt
    58921 ttctggaagc ggctcggctc gcgcagatac tctaccagtt cagcaacttc ttcttttgct
    58981 tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc
    59041 gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
    59101 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
    59161 agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
    59221 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
    59281 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
    59341 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
    59401 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
    59461 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
    59521 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
    59581 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
    59641 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
    59701 acaaaactgc cacctggcgc taatacatca cgacacattt ctaacgccag ttccaccaga
    59761 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
    59821 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
    59881 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
    59941 caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc
    60001 ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
    60061 atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca
    60121 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
    60181 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
    60241 ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc
    60301 aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc
    60361 gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat
    60421 tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga
    60481 aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt
    60541 catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg
    60601 ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca
    60661 ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt
    60721 tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat
    60781 tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt
    60841 gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc
    60901 ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt
    60961 tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg
    61021 cgcgttcgac agcttggctt cgatgtcttt aatacggcct tcgcagaaac cctgctgttc
    61081 acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat
    61141 agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtagttt
    61201 ttcagcgccg cgtaaggtca taggaatagc ttgcatttgt tatacctctt gaatattcct
    61261 gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg
    61321 gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt
    61381 ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc
    61441 gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt
    61501 tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta
    61561 cgttactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc
    61621 ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt
    61681 gattactgcg ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct
    61741 tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg
    61801 tgccgatccg acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc
    61861 tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga
    61921 taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc
    61981 tgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga
    62041 tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac
    62101 cctcccccga ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct
    62161 gaaccgcttt acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt
    62221 tgccgtgcag gatggagcca gctatgccgg ggcaattctg aaagatgagt taaaacaggc
    62281 aggtatcacc tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt
    62341 agttgccagt aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc
    62401 ggacaacatg atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc
    62461 tggaacatgg cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt
    62521 cgatattgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc
    62581 ccccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt
    62641 tatctccatg ctgccgcttg cgggctatga cggctctttg cagtaccgtg caggtctgca
    62701 tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa
    62761 cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc
    62821 tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga
    62881 aagccgtttg tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg
    62941 gccggggttt tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt
    63001 cccagtcatc atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat
    63061 gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga
    63121 ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac
    63181 tcgccgcaga gatcagataa tatttatctt cccagcccag cgcttcagca atcgctttcg
    63241 ctttctcttc agcttctgcc ttatccagca gatcgatctt attgaacact aaccaacgtg
    63301 gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt
    63361 caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac
    63421 gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca
    63481 gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac
    63541 ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcac
    63601 gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca
    63661 tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg
    63721 tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa
    63781 ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt ttcaccagta ccctggtcga
    63841 ttacacgcgt acctaccggc actttaattg tgacgtcttt accgcgctta ccggtacagt
    63901 cgcggcttgc gccattctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga
    63961 taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca ccgccgtcgc
    64021 cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac
    64081 cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg
    64141 taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc
    64201 aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat
    64261 aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat
    64321 ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct
    64381 gccagcgagc catcgcttcc gccagggcgc catatcctac caaggtattc agtccgcaaa
    64441 aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag
    64501 ggaagagcgc aattgtacat aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc
    64561 gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc
    64621 caaagataac tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt
    64681 taaaaaacat caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca
    64741 ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa
    64801 tcacctgcga agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc
    64861 caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc
    64921 gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca
    64981 agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga
    65041 ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct
    65101 gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca
    65161 tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta
    65221 ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt
    65281 taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac
    65341 ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca
    65401 gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt
    65461 tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc
    65521 gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata
    65581 gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac
    65641 ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt
    65701 tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc
    65761 caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc
    65821 gtacatataa aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat
    65881 attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa
    65941 tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta
    66001 caatcactct atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc
    66061 ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa
    66121 gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccagctgatc
    66181 aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct
    66241 gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg
    66301 atcgagttta tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg
    66361 cgcaggggta aagctaccgc caacgctgca tttggcaatg ccgccagcgt gctggtaggc
    66421 gattttattt atacccgcgc gttccagatg atgaccagcc tcggttcgct caaagtgctg
    66481 gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac
    66541 gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg
    66601 cgtctgtttg aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaagag
    66661 gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat
    66721 ttactcgatt acaatgccga tggcgaacag ttaggtaaaa atgtcggcga cgatctgaac
    66781 gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca
    66841 cagatgatcc gtaccgccat cgaacagggt aacggtcgcc atcttctgga accggttcag
    66901 gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga ggaagaagca
    66961 gacaaagcca tcgcagcatt acaggtgctc ccggacaccc cttggcgaga agcactcatc
    67021 ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccttcat cccgcgcagt
    67081 gcgcgggatg attccagtaa attccataaa aacactaatt cagcgctata aaacctgaaa
    67141 aaaagggcga tgaatcttta tatattctga atattcacac tctttacagg aactttttag
    67201 agcaataggc catcaggagt atagtgatgc ccgacagaag aagtgttctg aatgaaagcg
    67261 actaacttaa ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca
    67321 tcattgcggg tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt
    67381 tgagttcctc aacgctggca aacgcattat cgcgcccatg gccgaaagga gagatgatta
    67441 ttgcgaaagc cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc
    67501 agacccatga gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat
    67561 gagtggtctg gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt
    67621 tcacacgctc aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac
    67681 gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca
    67741 ccaggcttgc tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa
    67801 caccgtgaca aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct
    67861 ccggcacatg cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg
    67921 ccaccaggtt caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg
    67981 cggtacgtac gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt
    68041 cgccgacttc gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat
    68101 ctggctgcgc gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag
    68161 taccggtttc gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac
    68221 cttcgatgac gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga
    68281 agttcgcggt atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt
    68341 ccgccagggt tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca
    68401 cgatatgtgc gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt
    68461 tcatggtcgc gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga
    68521 tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca
    68581 gtgcccagat agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa
    68641 cgtcgcgggc atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta
    68701 gcagcttcat tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt
    68761 cttccgccag tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg
    68821 tgacttcgcc ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc
    68881 tcagttaaca attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt
    68941 tgcgatcgcg cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt
    69001 tatccgcaat atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca
    69061 tgccgtcaaa caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt
    69121 ggacttcctg gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg
    69181 gatcttcaat catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat
    69241 aaataagcaa aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc
    69301 ggacaggcgg ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag
    69361 gcaaattata caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt
    69421 tgttgccctg ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat
    69481 ccacgcggga cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca
    69541 tttcccaaag cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac
    69601 ccgtctgcat ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg
    69661 ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt
    69721 tccccactcg ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc
    69781 ctgccagttg cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg
    69841 gccattcggg tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg
    69901 ttctggcgca atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg
    69961 cttcaggtac tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt
    70021 gtaatactgg cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc
    70081 ctgatcaaca atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt
    70141 caggcgatcg aacgttttct gcgccgcctc gtccatcagc ttataaggat tggtctggtc
    70201 tgccgcggtt gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt
    70261 aaacatgcgt cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt
    70321 attacctggc gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc
    70381 gtaaaggaac tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt
    70441 atcgccatcc ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc
    70501 cagatattgt tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat
    70561 gtggttataa cgttgttcaa tttccagcgt tacgcgcggc agataggttt tcgggtccag
    70621 cgtaatatcc gccacccgac ccacaacaac gccaccaata ctgaccggag agcgcgcttt
    70681 caggccgcca atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga
    70741 cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa
    70801 gatacccacc caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg
    70861 cggtcagcac aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg
    70921 ttgcccggct aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc
    70981 acgtcaccgt gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc
    71041 agtcgacggc attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga
    71101 cgccgaccag agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta
    71161 atgaaataac cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca
    71221 tcatctccat actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg
    71281 ccgaaccagc acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta
    71341 gcgataacgc caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat
    71401 aaccttgcag ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg
    71461 acaggacgcc gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg
    71521 gtttgccgac cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt
    71581 taatcccttt atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct
    71641 ggtaaaagat cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct
    71701 atcccgtcca gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg
    71761 ccatgagcga caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact
    71821 tccggcacat cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa
    71881 atcagcttca ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac
    71941 atgatgagat ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca
    72001 ccagaaagtt cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc
    72061 atcatcactg tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag
    72121 gcgacgttgt caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc
    72181 atccgtttgc gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg
    72241 aaaaggatct caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt
    72301 ttaccgatgc ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa
    72361 atattatcga agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa
    72421 ttcgccacag actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg
    72481 ctcaatttaa ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag
    72541 ctggcatttg ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt
    72601 ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa ggacgttaga tgcttttagc
    72661 tacggcactg ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt
    72721 tgccgcgtct attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt
    72781 ggtcagtatt ggtacatcgt taccagaaat catcgtctcg cttgccgcgt ctctgcacga
    72841 acaacgcgat ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat
    72901 cctcggtctg gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga
    72961 attaccctta atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact
    73021 tagtcgcagc gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt
    73081 taaacttgca cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc
    73141 agagctgccg cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat
    73201 catcatgcca gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt
    73261 tgccatcagc gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga
    73321 actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat
    73381 cattggcgca aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc
    73441 aggagagatt gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat
    73501 tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg ggccgtggtg taggggtatt
    73561 attaactggc ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt
    73621 tgaataactg gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc
    73681 aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca
    73741 tcaatcagaa tttcacgctt gcttgtgaaa agatgttctg gtgtaaaggg aaagttgtcg
    73801 tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat ggcggcaacg tttgccagca
    73861 ccggtacacc ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg
    73921 ttaccccaca ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg
    73981 ccttaattcc agtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg
    74041 agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct
    74101 gtccgttagg gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc
    74161 tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc
    74221 caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg
    74281 atgagatccc gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc
    74341 gcaaaaatct tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta
    74401 ccgacggtga tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg
    74461 ccgatgtgat gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac
    74521 tgaacttaat gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac
    74581 tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata
    74641 aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga
    74701 cgttatggca aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc
    74761 agatggcctg atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga
    74821 cggttatggc attcgttgtg cgctcacctc tgatattgaa gtcgctatca ttaccgggcg
    74881 aaaggctaaa ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg
    74941 gcagtcaaac aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga
    75001 aaatgtggct tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt
    75061 aagcgtcgcc gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg
    75121 cattgctggc ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg
    75181 caaactggat gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt
    75241 gtgctatcac tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc
    75301 gcccaggtgg tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc
    75361 gtctataacc cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat
    75421 tccgatcagg ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa
    75481 atcccgacat ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat
    75541 ttatatggac acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg
    75601 acggataacg cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg
    75661 ttatacggaa caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag
    75721 aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag
    75781 ccttaatctt gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac
    75841 cggagacact gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg
    75901 caacgtggtt acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc
    75961 cgacaaagtg gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta
    76021 cggtaaaccg gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc
    76081 ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct
    76141 gcagcaggtt gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa
    76201 aatgcaggct ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct
    76261 gcaggacaaa aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat
    76321 ggcaacatta actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga
    76381 cgtcagcctg accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg
    76441 taagaccacc actttctaca tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat
    76501 tattgatgat gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg
    76561 ctatctgcca caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc
    76621 ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct
    76681 gatggaagag tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg
    76741 tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct
    76801 cgacgaaccg tttgccgggg ttgacccgat ctcggttatc gacattaaac gcatcattga
    76861 gcacctgcgc gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact
    76921 ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc
    76981 tacagaaatc ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact
    77041 ctgatagggt agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa
    77101 ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt
    77161 cgtctgttgc agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt
    77221 aatccgctgc ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac
    77281 agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag agatgccgga agagctgccg
    77341 ctcgatgcca gttgggacac catttacacc gctggtacac catccggcac cagcggtgac
    77401 tacattgacg atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac
    77461 ctgatgtggc aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct
    77521 atcgtcgatg ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa
    77581 agtatgggcg atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa
    77641 cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc
    77701 tcccaattcg ataaaaccac gccgtggctg gaagaggcca gactgatcat tagcgatcat
    77761 ctcgatctgt tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa
    77821 gatgtgctga aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg
    77881 atccagactg gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt
    77941 cactggacgg tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac
    78001 gcctcgatgt gcaataacgc gcgcaacgat gatgacagcc agtttatccg cagcaatctg
    78061 caggatgcca aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg
    78121 agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc aaggtgaaga atatatgaaa
    78181 ccgatggtac tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc
    78241 gtgaccacgc aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt
    78301 tccagtcacg tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcactg
    78361 gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc
    78421 tctttactgt cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag
    78481 tctttatcca ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga
    78541 agacactatg cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga
    78601 atttgttaca gctaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta
    78661 tgttgttctg aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg
    78721 tggtgaaatt catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat
    78781 tgataagctg gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc
    78841 cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt
    78901 aatgctccga gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa
    78961 taatgataca actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt
    79021 ccactgtcag agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact
    79081 tagcctgccg cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac
    79141 cggtatcggc aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc
    79201 cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc
    79261 ggtggactta ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac
    79321 tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc
    79381 ccagagcgat gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc
    79441 gtagttattc ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat
    79501 gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat
    79561 gggtttttac tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct
    79621 ggccgatcgc gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc
    79681 agaaatattc gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt
    79741 cctggatgcc gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc
    79801 gctttccagc aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga
    79861 gcctctgcgt tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct
    79921 ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt
    79981 tgagtctttc ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg
    80041 cttcttgccg aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc
    80101 tgtcgccgcg ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag
    80161 ctatcttgaa ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc
    80221 cattggttgt accggcggga agcaccgttc ggtgtatatt gcagagcaac tggcagacta
    80281 cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc
    80341 atgaccgtca agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca
    80401 atgaagctgt ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa
    80461 ggcaccgagg ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga
    80521 cggcagattg aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc
    80581 gccctcttta actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc
    80641 ttcaaactcc ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat
    80701 ttgtacattt tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagaggcatt
    80761 gtttaggttt tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga
    80821 gatggcatga ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt
    80881 tatgcgcatc cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt
    80941 ggcgataaag agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa
    81001 gactggagtg gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat
    81061 gtctacgatg tgattgcaga ttttgacggt aaaccctcca tta
//
LOCUS       DAHBHC010000011        80360 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHBHC010000011 DAHBHC010000000
VERSION     DAHBHC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.011"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(10..669)
                     /locus_tag="M2A35_000982"
     CDS             complement(10..669)
                     /locus_tag="M2A35_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCB7197314.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(833..1144)
                     /locus_tag="M2A35_000983"
     CDS             complement(833..1144)
                     /locus_tag="M2A35_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCB7197315.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            1189..2622
                     /gene="bglA"
                     /locus_tag="M2A35_000984"
     CDS             1189..2622
                     /gene="bglA"
                     /locus_tag="M2A35_000984"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCB7197316.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(2679..3422)
                     /locus_tag="M2A35_000985"
     CDS             complement(2679..3422)
                     /locus_tag="M2A35_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7197317.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(3683..6556)
                     /gene="gcvP"
                     /locus_tag="M2A35_000986"
     CDS             complement(3683..6556)
                     /gene="gcvP"
                     /locus_tag="M2A35_000986"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCB7197318.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(6675..7064)
                     /gene="gcvH"
                     /locus_tag="M2A35_000987"
     CDS             complement(6675..7064)
                     /gene="gcvH"
                     /locus_tag="M2A35_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCB7197319.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(7088..8182)
                     /gene="gcvT"
                     /locus_tag="M2A35_000988"
     CDS             complement(7088..8182)
                     /gene="gcvT"
                     /locus_tag="M2A35_000988"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCB7197320.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            8472..8555
                     /locus_tag="M2A35_000989"
     CDS             8472..8555
                     /locus_tag="M2A35_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197321.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(8629..9831)
                     /gene="ubiI"
                     /locus_tag="M2A35_000990"
                     /gene_synonym="visC"
     CDS             complement(8629..9831)
                     /gene="ubiI"
                     /locus_tag="M2A35_000990"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCB7197322.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGVRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYTYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(9854..11032)
                     /gene="ubiH"
                     /locus_tag="M2A35_000991"
                     /gene_synonym="visB"
     CDS             complement(9854..11032)
                     /gene="ubiH"
                     /locus_tag="M2A35_000991"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCB7197323.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRCAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(11029..12354)
                     /gene="pepP"
                     /locus_tag="M2A35_000992"
     CDS             complement(11029..12354)
                     /gene="pepP"
                     /locus_tag="M2A35_000992"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCB7197324.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(12380..12958)
                     /gene="ygfB"
                     /locus_tag="M2A35_000993"
     CDS             complement(12380..12958)
                     /gene="ygfB"
                     /locus_tag="M2A35_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCB7197325.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            13126..13455
                     /gene="zapA"
                     /locus_tag="M2A35_000994"
     CDS             13126..13455
                     /gene="zapA"
                     /locus_tag="M2A35_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCB7197326.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            13497..13680
                     /gene="ssrS"
                     /locus_tag="M2A35_000995"
     ncRNA           13497..13680
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="M2A35_000995"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13701..14303
                     /locus_tag="M2A35_000996"
     CDS             13701..14303
                     /locus_tag="M2A35_000996"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCB7197327.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(14404..14463)
                     /locus_tag="M2A35_000997"
     CDS             complement(14404..14463)
                     /locus_tag="M2A35_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCB7197328.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(14796..14855)
                     /gene="ibsC"
                     /locus_tag="M2A35_000998"
     CDS             complement(14796..14855)
                     /gene="ibsC"
                     /locus_tag="M2A35_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCB7197329.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(15084..16316)
                     /gene="serA"
                     /locus_tag="M2A35_000999"
     CDS             complement(15084..16316)
                     /gene="serA"
                     /locus_tag="M2A35_000999"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCB7197330.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(16572..17231)
                     /gene="rpiA"
                     /locus_tag="M2A35_001000"
     CDS             complement(16572..17231)
                     /gene="rpiA"
                     /locus_tag="M2A35_001000"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCB7197331.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(17287..17472)
                     /locus_tag="M2A35_001001"
     CDS             complement(17287..17472)
                     /locus_tag="M2A35_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197332.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            17614..18507
                     /gene="argP"
                     /locus_tag="M2A35_001002"
     CDS             17614..18507
                     /gene="argP"
                     /locus_tag="M2A35_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCB7197333.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18711..20855
                     /gene="scpA"
                     /locus_tag="M2A35_001003"
     CDS             18711..20855
                     /gene="scpA"
                     /locus_tag="M2A35_001003"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCB7197334.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            20848..21843
                     /gene="meaB"
                     /locus_tag="M2A35_001004"
     CDS             20848..21843
                     /gene="meaB"
                     /locus_tag="M2A35_001004"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCB7197335.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGSSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            21854..22639
                     /gene="scpB"
                     /locus_tag="M2A35_001005"
     CDS             21854..22639
                     /gene="scpB"
                     /locus_tag="M2A35_001005"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCB7197336.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            22663..24141
                     /gene="scpC"
                     /locus_tag="M2A35_001006"
     CDS             22663..24141
                     /gene="scpC"
                     /locus_tag="M2A35_001006"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCB7197337.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGEIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLHESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(24138..25034)
                     /locus_tag="M2A35_001007"
     CDS             complement(24138..25034)
                     /locus_tag="M2A35_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7197338.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKTHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(25201..25941)
                     /locus_tag="M2A35_001008"
     CDS             complement(25201..25941)
                     /locus_tag="M2A35_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCB7197339.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(26034..26669)
                     /gene="argO"
                     /locus_tag="M2A35_001009"
     CDS             complement(26034..26669)
                     /gene="argO"
                     /locus_tag="M2A35_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCB7197340.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(26808..27668)
                     /gene="mscS"
                     /locus_tag="M2A35_001010"
     CDS             complement(26808..27668)
                     /gene="mscS"
                     /locus_tag="M2A35_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCB7197341.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(28026..29105)
                     /gene="fbaA"
                     /locus_tag="M2A35_001011"
     CDS             complement(28026..29105)
                     /gene="fbaA"
                     /locus_tag="M2A35_001011"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCB7197342.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(29320..30483)
                     /gene="pgk"
                     /locus_tag="M2A35_001012"
     CDS             complement(29320..30483)
                     /gene="pgk"
                     /locus_tag="M2A35_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCB7197343.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(30533..31552)
                     /gene="epd"
                     /locus_tag="M2A35_001013"
     CDS             complement(30533..31552)
                     /gene="epd"
                     /locus_tag="M2A35_001013"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCB7197344.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            31924..32355
                     /locus_tag="M2A35_001014"
     CDS             31924..32355
                     /locus_tag="M2A35_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCB7197345.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFNRQPCALSGYDELHISPVK"
     gene            32378..32956
                     /locus_tag="M2A35_001015"
     CDS             32378..32956
                     /locus_tag="M2A35_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCB7197346.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            32957..33664
                     /locus_tag="M2A35_001016"
     CDS             32957..33664
                     /locus_tag="M2A35_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCB7197347.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSPLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            33652..34329
                     /locus_tag="M2A35_001017"
     CDS             33652..34329
                     /locus_tag="M2A35_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCB7197348.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            34323..35000
                     /locus_tag="M2A35_001018"
     CDS             34323..35000
                     /locus_tag="M2A35_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCB7197349.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(34972..35685)
                     /locus_tag="M2A35_001019"
     CDS             complement(34972..35685)
                     /locus_tag="M2A35_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCB7197350.1"
                     /translation="MNIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDKK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(35682..36191)
                     /gene="fumE"
                     /locus_tag="M2A35_001020"
     CDS             complement(35682..36191)
                     /gene="fumE"
                     /locus_tag="M2A35_001020"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCB7197351.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(36213..37178)
                     /gene="yggF"
                     /locus_tag="M2A35_001021"
     CDS             complement(36213..37178)
                     /gene="yggF"
                     /locus_tag="M2A35_001021"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCB7197352.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(37175..38452)
                     /locus_tag="M2A35_001022"
     CDS             complement(37175..38452)
                     /locus_tag="M2A35_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCB7197353.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(38467..39855)
                     /gene="cmtA"
                     /locus_tag="M2A35_001023"
     CDS             complement(38467..39855)
                     /gene="cmtA"
                     /locus_tag="M2A35_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCB7197354.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(39883..40326)
                     /gene="cmtB"
                     /locus_tag="M2A35_001024"
     CDS             complement(39883..40326)
                     /gene="cmtB"
                     /locus_tag="M2A35_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCB7197355.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(40640..42631)
                     /gene="tkt"
                     /locus_tag="M2A35_001025"
     CDS             complement(40640..42631)
                     /gene="tkt"
                     /locus_tag="M2A35_001025"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCB7197356.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTNFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            42909..43667
                     /gene="loiP"
                     /locus_tag="M2A35_001026"
                     /gene_synonym="yggG"
     CDS             42909..43667
                     /gene="loiP"
                     /locus_tag="M2A35_001026"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCB7197357.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(43723..44466)
                     /locus_tag="M2A35_001027"
     CDS             complement(43723..44466)
                     /locus_tag="M2A35_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCB7197358.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(44453..45562)
                     /gene="dgaE"
                     /locus_tag="M2A35_001028"
     CDS             complement(44453..45562)
                     /gene="dgaE"
                     /locus_tag="M2A35_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HCB7197359.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(45566..46426)
                     /locus_tag="M2A35_001029"
     CDS             complement(45566..46426)
                     /locus_tag="M2A35_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCB7197360.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(46423..47172)
                     /locus_tag="M2A35_001030"
     CDS             complement(46423..47172)
                     /locus_tag="M2A35_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCB7197361.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(47198..47683)
                     /locus_tag="M2A35_001031"
     CDS             complement(47198..47683)
                     /locus_tag="M2A35_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCB7197362.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(47694..48122)
                     /locus_tag="M2A35_001032"
     CDS             complement(47694..48122)
                     /locus_tag="M2A35_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCB7197363.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(48241..51039)
                     /gene="dagR"
                     /locus_tag="M2A35_001033"
     CDS             complement(48241..51039)
                     /gene="dagR"
                     /locus_tag="M2A35_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HCB7197364.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(51298..52218)
                     /gene="speB"
                     /locus_tag="M2A35_001034"
     CDS             complement(51298..52218)
                     /gene="speB"
                     /locus_tag="M2A35_001034"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCB7197365.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(52354..53085)
                     /locus_tag="M2A35_001035"
     CDS             complement(52354..53085)
                     /locus_tag="M2A35_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197366.1"
                     /translation="MKKWKVRSALVALVVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINQLEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(53231..55207)
                     /gene="speA"
                     /locus_tag="M2A35_001036"
     CDS             complement(53231..55207)
                     /gene="speA"
                     /locus_tag="M2A35_001036"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCB7197367.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(55216..55347)
                     /gene="yqgB"
                     /locus_tag="M2A35_001037"
     CDS             complement(55216..55347)
                     /gene="yqgB"
                     /locus_tag="M2A35_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCB7197368.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            55483..55698
                     /locus_tag="M2A35_001038"
     CDS             55483..55698
                     /locus_tag="M2A35_001038"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197369.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            56002..57156
                     /gene="metK"
                     /locus_tag="M2A35_001039"
     CDS             56002..57156
                     /gene="metK"
                     /locus_tag="M2A35_001039"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCB7197370.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            57593..58987
                     /gene="galP"
                     /locus_tag="M2A35_001040"
     CDS             57593..58987
                     /gene="galP"
                     /locus_tag="M2A35_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCB7197371.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            59064..59561
                     /locus_tag="M2A35_001041"
     CDS             59064..59561
                     /locus_tag="M2A35_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCB7197372.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            59656..60363
                     /gene="endA"
                     /locus_tag="M2A35_001042"
     CDS             59656..60363
                     /gene="endA"
                     /locus_tag="M2A35_001042"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCB7197373.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            60443..61174
                     /gene="rsmE"
                     /locus_tag="M2A35_001043"
     CDS             60443..61174
                     /gene="rsmE"
                     /locus_tag="M2A35_001043"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCB7197374.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            61187..62137
                     /gene="gshB"
                     /locus_tag="M2A35_001044"
     CDS             61187..62137
                     /gene="gshB"
                     /locus_tag="M2A35_001044"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCB7197375.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            62246..62809
                     /locus_tag="M2A35_001045"
     CDS             62246..62809
                     /locus_tag="M2A35_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCB7197376.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            62809..63225
                     /gene="ruvX"
                     /locus_tag="M2A35_001046"
     CDS             62809..63225
                     /gene="ruvX"
                     /locus_tag="M2A35_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCB7197377.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(63401..64381)
                     /locus_tag="M2A35_001047"
     CDS             complement(63401..64381)
                     /locus_tag="M2A35_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCB7197378.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            64399..65103
                     /gene="yggS"
                     /locus_tag="M2A35_001048"
     CDS             64399..65103
                     /gene="yggS"
                     /locus_tag="M2A35_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCB7197379.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRRVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            65121..65687
                     /gene="yggT"
                     /locus_tag="M2A35_001049"
     CDS             65121..65687
                     /gene="yggT"
                     /locus_tag="M2A35_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCB7197380.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            65684..65974
                     /locus_tag="M2A35_001050"
     CDS             65684..65974
                     /locus_tag="M2A35_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCB7197381.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            65982..66575
                     /locus_tag="M2A35_001051"
     CDS             65982..66575
                     /locus_tag="M2A35_001051"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCB7197382.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            66568..67704
                     /gene="hemW"
                     /locus_tag="M2A35_001052"
     CDS             66568..67704
                     /gene="hemW"
                     /locus_tag="M2A35_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCB7197383.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(67859..68866)
                     /locus_tag="M2A35_001053"
     CDS             complement(67859..68866)
                     /locus_tag="M2A35_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCB7197384.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(68983..70029)
                     /gene="ansB"
                     /locus_tag="M2A35_001054"
     CDS             complement(68983..70029)
                     /gene="ansB"
                     /locus_tag="M2A35_001054"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCB7197385.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(70205..70924)
                     /locus_tag="M2A35_001055"
     CDS             complement(70205..70924)
                     /locus_tag="M2A35_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCB7197386.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(71108..71434)
                     /locus_tag="M2A35_001056"
     CDS             complement(71108..71434)
                     /locus_tag="M2A35_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCB7197387.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(71434..72153)
                     /gene="trmB"
                     /locus_tag="M2A35_001057"
     CDS             complement(71434..72153)
                     /gene="trmB"
                     /locus_tag="M2A35_001057"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCB7197388.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            72314..73366
                     /gene="mutY"
                     /locus_tag="M2A35_001058"
     CDS             72314..73366
                     /gene="mutY"
                     /locus_tag="M2A35_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCB7197389.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            73394..73669
                     /locus_tag="M2A35_001059"
     CDS             73394..73669
                     /locus_tag="M2A35_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCB7197390.1"
                     /translation="MSRTIFCTFLQHEAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            73734..74813
                     /gene="mltC"
                     /locus_tag="M2A35_001060"
     CDS             73734..74813
                     /gene="mltC"
                     /locus_tag="M2A35_001060"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCB7197391.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            75015..76271
                     /gene="nupG"
                     /locus_tag="M2A35_001061"
     CDS             75015..76271
                     /gene="nupG"
                     /locus_tag="M2A35_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCB7197392.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFRVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(76321..78456)
                     /gene="speC"
                     /locus_tag="M2A35_001062"
     CDS             complement(76321..78456)
                     /gene="speC"
                     /locus_tag="M2A35_001062"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCB7197393.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            78854..79561
                     /locus_tag="M2A35_001063"
     CDS             78854..79561
                     /locus_tag="M2A35_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCB7197394.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            79667..79742
                     /locus_tag="M2A35_001064"
     tRNA            79667..79742
                     /locus_tag="M2A35_001064"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79700..79702,aa:Phe,seq:gaa)
     gene            79940..>80360
                     /locus_tag="M2A35_001065"
     CDS             79940..>80360
                     /locus_tag="M2A35_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCB7197395.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTL"
BASE COUNT        19451 a        21634 c        20094 g        19181 t
ORIGIN      
        1 gcatacccat tacgcctgcc caatatacaa atagatcgcc agaaagtggc acacactacc
       61 gccgagcacg aagccgtgcc agatggcatg gttgtatgga atgcgtttgc agacgtagaa
      121 aatcaccccg agcgaataaa ccacgccgcc taccgccagt aaggtaacgc tgcccgccgc
      181 gagcttaact gccatttcat aaattaccac cagcgacagc cagcccatcg ccagataggt
      241 caccagagat aaaattttga atcggtgcgc gatggtcagt ttaaacagaa tccccagcaa
      301 tgccaggctc cagataacaa tcatcaaccc gcgcgctaac ggagaatcca gccccaccag
      361 caaaaacggc gtgtaggttc cggcaatcaa caggtaaata gcgcaatggt caaatttctt
      421 cagccacatt tttgcccgtt gatgaggaat ggcgtgatag agcgtcgaag cgaggaacag
      481 caggatcata ctgccgccat agaggctgta gctggttatc gctgtggcgc tggcattgag
      541 atccaccgcc tgaaccagta gcaacaccag cccaacgata ccaaacacca acccaatgcc
      601 gtgactgacg ctgttggcaa tttcctctgc cagcgaatat ccctgcttaa tgaggggctt
      661 ctgaaccata acttactccg gagaaacgta cacgcacatg tatacctctc cagcgtaact
      721 gagaatagtt ccagtgaaca cctgttagct aaaataaatt ctgatttgag actaatctcc
      781 taaaaatcat gaaattaaat gagaaatttc aactaacagg cgtgagttca atttaaagac
      841 atttaaattc aatcacataa aactgtgtct gaccgggata gatgtcggca atgacctttt
      901 tcagttcagt cagggtcata ttttcctgct ctgcatgttt ttcagtcagc gtatccagcc
      961 ttacggttga ggttgcggtg acttcaatcg tgcaaaaata accgtcatct tcaaaacgcc
     1021 cgacacgaag cacatcaccc gttttgaagt gcgattcaga ctcgtcgcgg atggtgatgg
     1081 ttttacgccc agccagaatg tcatcctgga aacgttgaaa aaaagtgatg tcgtttggct
     1141 gcatggtact atttcctgta agaattgact catctggagc ctatgatagt gaaaaaactc
     1201 accttaccga aagatttctt atggggcggc gcagttgccg ctcatcaggt cgaaggcagc
     1261 tggaacaaag gcggcaaagg gccgagcatt tgtgacgttc tgaccggtgg cgcacacggc
     1321 gtgccgcgcg aaatcaccaa agaagtcttg ccaggcaaat actatccaaa ccatgaagcc
     1381 gttgattttt atggtcacta caaggaagac atcaagctat ttgccgaaat gggcttcaaa
     1441 tgttttcgga cttccatcgc ctggacgcgt atttttccaa aaggtgatga agctcagcca
     1501 aacgaagaag ggctgaagtt ctacgatgat atgttcgatg aactgctgaa atacaacatc
     1561 gaaccggtga tcaccctctc ccactttgaa atgccgctgc atctggtgca gcaatacggt
     1621 agctggacca accgtaaagt ggttgatttc tttgtccgtt tcgcggaagt ggtgtttgaa
     1681 cgctataagc ataaagtcaa atactggatg accttcaacg aaattaacaa ccagcgtaac
     1741 tggcgtgcac cgctgttcgg ttactgctgc tccggcgtgg tttataccga gcatgacaac
     1801 ccggaagaga cgatgtacca ggtgctgcat caccagtttg tcgccagcgc cctggcggtg
     1861 aaagctgcgc gtcgcattaa cccggagatg aaagtcggct gtatgctggc gatggtgccg
     1921 ctctatccct actgctgtaa cccggacgat gtgatgttcg ctcaggagtc gatgcgcgaa
     1981 cgctacgtct ttaccgatgt gcagctacgc ggctattacc cgtcctatgt gttgaacgag
     2041 tgggagcgtc gcggatttaa catcaaaatg gaagacggcg atctcgatgt actgcgtgaa
     2101 ggcacctgcg attatcttgg tttcagctat tacatgacca acgcagtgaa ggccgaaggc
     2161 ggcaccggcg atgcgatctc tggtttcgaa ggcagcgtac cgaacccgta tgttaaagcg
     2221 tctgactggg gctggcagat tgatccagta ggtctgcgct atgccctttg cgaactgtat
     2281 gagcgttatc agaggccgct gtttattgtc gaaaacggtt ttggcgctta cgacaaagtg
     2341 gaagaagatg gcagcatcaa cgacgactac cgcattgact acctgcgcgc ccatatcgaa
     2401 gagatgaaaa aagcggtgac ttacgatggc gtggatctga tgggctacac accgtggggc
     2461 tgcatcgact gcgtatcgtt caccaccggg cagtacagca aacgctacgg ctttatctat
     2521 gtgaataaac atgacgacgg tgctggcgat atgtcgcgtt cacgtaagaa gagctttaac
     2581 tggtaccaag aggtgattgc cagcaacggc gagaagcttt aagtcgatga agtaccggat
     2641 gcaatacttg ttgcatccgg tcagacaact tccctgtttt atttcccgcc cgccaaatcg
     2701 ataaaacttc ccgtgacgta agaggcttta tcacttagta gccagacaat ggcctgcgcg
     2761 acctcttctg cctgtccacc acgctgcatg gggatgttcg acttaacgcg atcgacgcgt
     2821 ccaggctcgc cgccgctggc gtgcatttcg gtataaataa accctggccg cacgcagtta
     2881 acgcggatcc cctgcgcggc gacttccagc gatagtccgg tggttaacgt atcaatcgcc
     2941 cctttcgatg ccgcgtaatc aacatattcc cctggcgaac ccaaccgtga ggccaccgaa
     3001 gagacattga cgatagcgcc gccactgcca ccatttttaa gcgccatgcg ttttaccgcc
     3061 tcgcggcagc agagaaaata tcccgtcacg ttggtggaaa gtactcggtt gattcgctct
     3121 gcggtaaggt tttcaacggt gcactgggta aacaagatcc cggcgttatt gaccagcgct
     3181 gctagcggtt catcgtgctg atcgattgct gtaaacatcg caacgacctg gttttcgtcg
     3241 ctgatatccg cctggagcac gaatgctttg ccaccggctt gcgttattaa gttcatcact
     3301 tcctgcgccg cgtggaggtt ttgctgataa ttaaccgcca ccgtataccc ttcttgcgcc
     3361 aacagtaatg cagttgcccg cccgatgccg cgactgccac cggtcacaag tgctataacc
     3421 atttgttttt ccgagaaaaa ttcattcgtt gacgtatctt tgagattgct aacaaactgg
     3481 ctcaatcttc aatgttggca cgcttttccc cctcgccctt tcagggagag ggccggggtg
     3541 agggtaaata ttcgcgccag tgccggcctg ttcccctcac cctctcccca aaggggcgag
     3601 gggactgtct gagcactttt gtactttgtc atctgactaa aaaggcgccg aagcgccttt
     3661 agaaaatagt cgaatcagtg aattactggt attcgctaat cggtacgcag gagcagaaca
     3721 ggttacggtc gccgtaaaca tcatccagac gtttcaccgt cggccagtat ttgtctgcca
     3781 cacctgccgg gaatactgca acctcacggc tgtacggatg cgcccactcg gcgaccagtt
     3841 cgttctgaat gtgcggcgcg ttcaccagcg ggttatcttc cagcggccag acaccggctt
     3901 taacctggtc gatttctgcg cggatagcca gcatcgcgtc gataaagcga tccagttcca
     3961 ctttgctttc agattcagtc ggttcaacca tcagcgtacc cgccaccggg aacgacatgg
     4021 tcggcgcgtg gaaaccgtag tcgatcaggc gcttggcaat atccagctcg ctgatgccgg
     4081 tttcttcttt cagcgggcga atatcgagaa tacattcgtg cgccacgcga ccgtcgcgac
     4141 cggtatacag caccgggaag gcatcctgca ggcggctggc aatatagttg gcgttgagga
     4201 ttgccacctg gcttgctttt ttcagccctt ctgcgcccat catgcggatg tacatccagc
     4261 tgattggcag gatagaggcg ctaccgaacg gtgccgcaga aactgcgccc tgacgggtta
     4321 acatgccttc gatttgcacc acgctatgac ccggtacaaa cggtgccaga tgcgctttca
     4381 caccgatcgg tcccataccc ggaccaccac cgccgtgcgg aatgcagaaa gttttatgca
     4441 ggttgaggtg cgaaacgtcc gcgccaataa agcccggcga ggtgatgcca acctgggcgt
     4501 tcatgttcgc gccatcaagg taaacctgac cgccgaactg atgcacgact tcacacactt
     4561 cacggatcgt ttcttcatac acgccgtggg tagaaggata agtcaccatg atacaggaga
     4621 ggttatcgcc cgcctgttcc gctttcgcgc gcagatcagt cagatcgatg ttgccgtttt
     4681 tatcacacgc cacaaccacc acctgcattc ctgccatatg tgcagaagcg gggttagttc
     4741 cgtgcgcaga agccgggatc aggcagatat cgcgatgccc ttcgttgcgg ctttcatgat
     4801 aatgacgaat cgccagcagg cccgcgtatt cgccctgtgc gccggagttc ggctgcatac
     4861 aaacggcgtc gtaaccggtc agtttcacca gccagtcagc cagctgcgca atcatctgct
     4921 gataaccttc agcctgttcc ggcggacaaa acgggtgcag ttcggcaaat tccggccagg
     4981 tgattgggat catctcggcg gcggcgttca gtttcatggt gcaggaaccc agcgggatca
     5041 tcgcctgatt cagcgccaga tctttacgct ccagcgagtg catatagcgc atcatttcgg
     5101 tttcgctgtg gtagcgatta aacaccggat gggtgaggat ttcgtcgtcg cgcagcatcg
     5161 caggctggat agagcggctg tcgtgagcca cgtctttgtc cagcgtgtcg atgtccaggc
     5221 cgtggttatc gcccagcagc acgttgaaaa gctgcattac gttttcacgc gtggttgttt
     5281 catcaagggt gatcccaacc gcgttcagaa tatcgctacg caggttgatt tcagccgctt
     5341 cggcacgcgt cagtacgccc gctttgtcgg ccacttccac acacaaggtg tcgaaatagt
     5401 gcgcatggcg cagtttcaga cctttttgtt gcaggcccgc cgccaggata tcggtcagac
     5461 ggtgaatgcg gttagcgata cgtttcaggc caatcgggcc gtgataaacg gcatacaggc
     5521 tggcgatgtt tgccagcagc acctgggaag tacaaatgtt ggagttcgct ttctcacggc
     5581 gaatatgttg ctcgcgagtc tgcatcgcca tgcgcagcgc ggtattgcca gctgcatctt
     5641 tcgatacacc gataatacgg cccggcattg agcgtttgta ttcatctttc gccgcaaaga
     5701 aggccgcgtg tgggccaccg tagcccatcg gcacgccgaa gcgttgcgcc gaaccaaaaa
     5761 caatatccgc gccctgttta cccggcgcag ttaacagcac cagcgccata atatcggcgg
     5821 cgacactgac cacaattttg cgtgatttca gttcgctaat aagcgcggtg tagtcgtgga
     5881 tttcaccggt tgtgccaacc tgctgtaaca gcacgccgaa gacgtcctga tggtcgagca
     5941 ctttttgcgc gtcatcgaca atcacttcaa aaccaaaggt ttcggcacga gtacggacca
     6001 catccagcgt ttgcggatgc acatcggaag ccacgaagaa gcggttggca tttttcagtt
     6061 tgctgacgcg tttcgccatc gccattgctt cggcggcagc ggtggcctcg tccagaagag
     6121 aagcagaggc catatccagt ccagtcaaat ccagcgttac ctgctggaag ttgagcagtg
     6181 cttcaaggcg gccctgggag acttcaggtt gatacggagt gtacgcggta taccagcccg
     6241 gattttccag catgttacgc aggataaccg gcggtagctg cacggcggtg taacccatgc
     6301 cgatgtaaga cgtgaagcgt ttattgcgac tggcaatagc cttgagttct gccagtgcgg
     6361 cgtattcggt cgccggtgcg ccaacctgcg gcggggtcgc aagctgaata tctttcggca
     6421 caatctggcc ggtcagcgcg tttaacgatt gtgcaccaac ggcattcagc atttcttgct
     6481 gttgcgcggc gtccggtccg atatggcgtt caataaaagc gccgctgttt tcaagctggc
     6541 ttaacgtctg tgtcatgagc gatggttcct gaaacgtgca gtgaattgtg aacctctctc
     6601 cttacgaaga gagtgagggt gaggcataaa ttttcctcac cctgatcctc tcccgcagaa
     6661 gaggaataaa gccgttactc gtcttctaac aatgcttcgt atgcggtcgc atccagcagt
     6721 gattccagtt cgctttcatc gctggctttg attttaaaga tccagccacc tgcatacggt
     6781 tcgctgttca ccagttccgg ggaatcgctc agtgcgtcgt ttaccgccac gatttcaccg
     6841 cttactggcg cataaatgtc tgacgccgct tttactgatt cggcaaccgc gcagtcatcg
     6901 cccgcgctaa ccgttgcgcc cacttccggc aggtcaacaa acaccatatc gcctaacagc
     6961 tcctgggcat gttcggttat accaacggtg taagtgccgt cggcttcttt acgcagccat
     7021 tcgtgttctt tgctgtattt cagttctgct ggtacgttgc tcatcaatca atctccaaaa
     7081 aagtaaatca cgcgacggct ttaccgttac gcacaaaaac aggtttcgtc actttaaccg
     7141 gcatttcacg gttgcgaatt tgcacaatcg ccgtttcgcc aataccttcc ggcacgcgtg
     7201 ccagcgcaat gctgtaaccc agcgtcgggg agaaagtccc gctggtgata atgccttcat
     7261 gctggttgcc ctgcgcatcg gtaaaacgta ccggcagttc attacgcagc acgccttttt
     7321 cggtcatcac cagaccaacc agtttttctg taccatgctc acgctgcact tccagggctt
     7381 cacgaccgat aaagtcacga tctgccggtt cccaggcgat agtccagccc atgttggcgg
     7441 ctaaaggaga aatagtttcg tccatctcct gaccataaag attcatgccc gcttccagac
     7501 gcagcgtgtc acgcgcgccc aagccacatg gcttaacacc cgcttccacc agcgcacgcc
     7561 agaaatcggc cgctttttca ttgggcagcg caatttcata gcccgcttca ccggtataac
     7621 cagtggtggc aataaacaga tcgcccgcct gcacgccaaa gaacggtttc atcccttcca
     7681 ccgcctgacg ctgggcgtca ttaaacagtg tggcagcttt tgcctgcgca ttcggccctt
     7741 gcacggcaat catggaaagg tcatcacgaa cggtaatttc gatgccgaaa ggttcagcgt
     7801 gttgggtaat ccaggagagg tctttttcgc gggtggcgga gttaacaacg aggcggaaga
     7861 aatcttcagt aaagtagtag acgatgaggt catctatcac accgccagag gcattcaaca
     7921 tccccgagta aagggctttg ccgcttttgg tgagcttcgc cacatcgttc gccagcagat
     7981 aacgcagaaa ctcccgggtg cggctgccgc ggagatcgac gatggtcata tgtgacacat
     8041 caaacattcc ggcatcggta cgtaccgcat gatgttcgtc gatttgcgaa ccgtaatgta
     8101 gcggcatcat ccagccgtgg aaatccacca tgcgagcgcc gcaaagcgtg tgttgttcgt
     8161 acaaaggagt ctgttgtgcc atcttgtcct cattgaataa gcggggctga caacttttca
     8221 tggtgaaatt atcaccacga aacccagcat cggagccact cccggtcccc aacgcaatcg
     8281 ttctcttttg cctgaactta ccaccgaaac agactgttaa ccataaggta aaattgatca
     8341 tcacattagc ttatggttaa aaaacgccaa aatcgcgaca gaataaaaaa ccaaaaaaca
     8401 caccagtttc tatataaaga tgatgccatt agaaagctaa tttgaataag gcaatattaa
     8461 gagctaaaaa aatgtcaaaa aacactaaat caaaaaataa tgacattaga aaatataatg
     8521 cgaaaacgga ggtgaaatta gtttatttca aatgaggaaa atctcccggc gaaaaaaccg
     8581 ggagatgaaa gtgtgatggg tatcaaataa acagcagaag agaaattttt aacgcagcca
     8641 ttcaggcaaa tcgtttaatc ccattgcctg gcggataagt tgtggtttaa cgccaggaag
     8701 cgtgtcggcc agtttcaaac caatatcacg cagcagtttt ttcgccggat tggtaccgga
     8761 aaacagatcg cggaatccct gcataccagc cagcatcagc gccgcactgt gcttgcggct
     8821 acgctcatac cggcgcaggt aagtgtactg cccgatgtct ttcccctgac gatgcaaccg
     8881 tttcagttca gcaatcagct ctgcagcatc cataaagccg agatttactc cctgccccgc
     8941 cagcgggtga atggtatgcg cggcgtcgcc caccagcacc agacggtgcg cggcaaactg
     9001 gcgcgcataa cgccccgtca gtgggaacac ctgacgcgcg ctctcaacct tgcataagcc
     9061 caggcgatta tcaaaagcga tatttaacgc gcgattaaat tcatcttcac ttgcctgctg
     9121 catccgctgc gcttcctctg gcgacagtga ccagacaatt gagcaaagat gcggatcgct
     9181 aagcggtaaa aaggccagaa tgccttcgcc atggaaaacc tgccgcgcca ccgcatcatg
     9241 cggttcttcc gtgcgaatgg tcgctaccag cgcgtgatgc tgataatccc agaaagtcag
     9301 cggaatatct gctttgttgc gcaaccagga attagcaccg tccgcgccaa tcaccagacg
     9361 cgccgttaac atgctgccat ctttcagcgt caggaaagtt tcattttctc cccaggcgac
     9421 ctgctgtaat tctgcggggg ccaacagagt gatatctgac gactgctgcg ctttgttcca
     9481 caacgcgtag tgaatcactg agttttcaac gatatgccca agatggctat agcccatgct
     9541 ttgatcgtca aacgaaatgt gaccaaaact gtctttgtcc cacacttcca taccgtgata
     9601 acaactggcc ctacgagaga gaatgtcctg ccagacgcca agacgggtga gtaatttttc
     9661 gctggcggca ttgatagccg aaacgcgcag ttgtggtggt gcatccgccg ccagaggttc
     9721 cggtacgcgc tgctccagta cggcaacgcg tacgccgctc ccctgtaagc cgcaggcaac
     9781 cgccagcccc accatgccgc caccaacaat ggctacatca acactttgca ttgtttattc
     9841 cttaaaaccg ccttcaacgc gccacccaac cgagggtgcg ctgcgccagc acatcgcgtg
     9901 ccggggtgaa taattccatc gtcatcagtc cgatgttgcg cccgacaacc agcggtgccc
     9961 aacggttggc aaaaagatgt accaggctgt ccgtgacgcc gatggttgct tcgcgatcgc
    10021 tctgtcgacg ctgttgatag cggcacaata tgccgtaatc accgatgtct tctccgcgct
    10081 catgcgcctg agttagggtt tccgcaaggc tcatgacatc tcgcataccg aggttaaacc
    10141 cttgcccggc aatcgggtgc agagtttgcg ccgcattgcc caccagcacg gtacgatggg
    10201 taatagctct ggcggcgcgg gttaacgcca gcggataagc acagcgttta ccggcgtggg
    10261 taatttttcc aagtcgccag ccaaaggccg actggagttc acggcaaaac ttctcgtcgc
    10321 tccacgacag cacctcttcg cgccgttcca gtggatgaca ccagaccagc gaacagcgtc
    10381 cgtcagacat cggcaacatc gccagcgggc catgttgcgt gaagcgttca aaagcgcggc
    10441 cttcgtgcgc aacggaagtc gcaacgttgg caatcacggc cagttgttcg taaggctcct
    10501 gctgccaatc aacgccgcac gcggtggcta acgctgaatg ggtgccatca gcggctatca
    10561 gcacgcggcc cgtcagcatc tcgccgctct ccagcgtcac ttcaacgtga ctctgagtac
    10621 gggcaacgtt agctacgcga tcggggcaat gcagcattac gccaggtgct tttcgcagca
    10681 atgcaaacag ccgttgcccg acattatgca attcgacaac ctgccccagc gccgccagtt
    10741 ggtaatcttc tgcggcgagg gtgacgaatc cagcatgacc acgatcgctg acatgcacgg
    10801 tagtgatggc ggttgcgcaa tcagccagag attgccagac gccgatgcgc gccagttgct
    10861 gacaggtccc cgccgctagc gcaatagctc gtccatcaaa gcccggatga gcatgtgact
    10921 ctggcgcggt cgcttcaatc aaatgtaccg gcagcgcccc gtgacttaac cgggaaatag
    10981 ccagcgccag cgtcgcgccc gccatgccgc caccgacgat gattacgctc attgctttct
    11041 cgcagctgcc atcaacgctt cgatttcttc cggctttttc accacgctgg cggtgaggtt
    11101 ttcgttaccg gtttcggtaa tcacaatgtc gtcttcaata cgaatgccga taccgcgata
    11161 ttgttctggc acttctgcat ccggcgcaat atacagccct ggctctacgg tcagtaccat
    11221 gcccggttcc agaatgcgcg agcgatcctg accataaacg ccaacgtcat ggacatctag
    11281 ccctaaccag tggctaaggc catgcataaa gaaaggacga tgggcgttct gagcgatcag
    11341 ttcatcaaca tcacctttca gaatgccgag ttttaccagg ccgctaacca tgatgcgcac
    11401 cacttcaccg gtgacttcct gcatggaagt tcccggacga tacaggcgca agctggtttc
    11461 gagagactcc agcacaatgt cgtagatttc acgctgggcc tgagtgaatt tgccgttgac
    11521 cgggaaggtg cgggtaatat cgccagcgta acctttgtat tcacaacccg cgtcaatcaa
    11581 caccaggtca ccgtcacgca gttcacactc gttttcggtg tagtgcaaaa tgcagccgtt
    11641 ttcaccgctg ccgacaatgg tgttatagga aggatagcgc gcgccgtggc ggttaaattc
    11701 gtggtgaatt tcgccttcca ggtggtactc gaacattccc ggacggcatt tttccatcgc
    11761 ccgggtatgc gccagggcgg tgatttcacc cgcgcggcga agtacggcaa tctcttctgg
    11821 cgatttgaac aggcgcattt catgaacaat gggacgccag tcgatcatcg ttgccggcgc
    11881 ggtgagattt tgtcgcgaac ctttacgcag cttttccagc gcgctgttca cgatttcatc
    11941 agcataggta tattcgccct gggcgtggta aaccacatcc agaccattaa gtaattgata
    12001 aagttgctga ttaatttcgc tgaatgccag tgcgcggtca acgcccagtt tctctggcgc
    12061 ggcatcctga cctaaacgac ggccaaacca gatctccgcc gtcaggtcgc gaacgcggtt
    12121 aaacagaacg ctgtggttat gagtgtcatc gcttttaatc agcaccagca ccgcttccgg
    12181 ttcgttaaag ccggtgaagt accagaagtc actgttctga cgataggggt attcgctgtc
    12241 ggcgctacgt gttacttctg gtgcagcaaa aatcagcgcg gcgctgccgg gttgcatttg
    12301 ctccaccagg gcctgacggc gacgctgaaa ctcttgccgg gatatctcac tcataacact
    12361 ctccttacgt tttttgtttt tagtgtagag tcggtttttg tacttctggc gcggtcggtt
    12421 gcggatgagt aaaggtgtcg tggcataaca gcgcggcaac acgaacgtat tcgatgatct
    12481 cttcaagcga catttcaagc tcttcctgat cttcgtcttc gtcgtaaccc agttgcgcaa
    12541 tgttacgcag atcgtcgata gcttcaccgg tttcgccggt cactttatcc agcttcggtt
    12601 gcgtaacgcc aagaccaagc aggaagtgat tgacccaacc tgccaatgca tcagcccgat
    12661 cgaaaacgct gacatcatcg ccatcaggca gataaagctg aaaaaggaag ccgtcatcct
    12721 gcagggcatc gctggtggca gagtgcattt tacgcagtgc ctgtgccagc tcatgaccga
    12781 aagccatgcc ttcgttcgtc aggtcgtgaa gtagcggtag ccatgagctg tcatcgttac
    12841 cgccacatat catcccactg attaaaccat gcatctcagc aggggtcaga cccgtccctt
    12901 gttggttcag atactggttc atttcgttgt aaccaggcat ttcgttctgt atagacataa
    12961 gcattcgtcg tcaaagggag gaatattcat gatatgctac cactttgggc cctggtggac
    13021 cagaaaaggg cttgtctctt ctcatcaggg tagctatagt gtcgcccctt cgcagaccat
    13081 gggtctaaag acgaaggcag cgcagtcaat cagcaggaag gtggcatgtc tgcacaaccc
    13141 gtcgatatcc aaatttttgg ccgttcactg cgtgtgaact gcccgcctga ccaaagggat
    13201 gcgttgaatc aggcagcgga cgatctgaac caacggttgc aagatctgaa agaacgcact
    13261 agagtcacaa atactgaaca gttggtcttc attgccgcat tgaatatcag ctatgagtta
    13321 gcgcaagaaa aagcgaagac tcgtgactac gcggcaagta tggaacagcg tattcggatg
    13381 ctgcagcaga ccatagaaca agcgttactt gaacaaggtc gcatcaccga aaaaactaac
    13441 caaaactttg aatgacactt ttcggtttac tgtggtagag taaccgtgaa gacaaaattt
    13501 ctctgagatg ttcgcaagcg ggccagtccc ctgagccgat atttcatacc acaagaatgt
    13561 ggcgctccgc ggttggtgag catgctcggt ccgtccgaga agccttaaaa ctgcgacgac
    13621 acattcacct tgaaccaagg gttcaagggt tacagcctgc ggcggcatct cggagattcc
    13681 cttcttatct ggcaccagcc atgacgcaac taccagaact cccactaaca ttatcccgac
    13741 aagaaatccg caaaatgatt cggcaacgtc gtcgtgcgtt aacgccggaa caacagcagg
    13801 aaatgggtca acaagccgct acccggatga tgacttatcc cccggtggtg atggcgcata
    13861 cggtcgctgt attcctctct tttgatggtg aactcgacac ccagccgcta atagaacaac
    13921 tctggcgcgc cggtaagcgc gtatatcttc cagttttgca tccctttagt gccggtaatt
    13981 tgctgttcct gaattaccat ccgcaaagcg aactggtgat gaacaggttg aagatccatg
    14041 agccaaaact ggatgtgcgt gacgtgctac ccctttcccg attagacgtg ctgatcacac
    14101 cgctggtcgc ctttgatgag tacggtcagc gcctgggaat gggcggtggt ttttatgatc
    14161 ggaccttaca aaactggcag cactataaaa cgcaaccggt gggttatgcg catgattgtc
    14221 agttggtgga aaaactcccc gttgaagagt gggatatccc tcttcctgcg gtggttacac
    14281 cgtcgaaagt ctgggagtgg taagggcgat acacctgcat cgccctgatt gacatcgttg
    14341 attctttaac ctaatttagt gagtaagggt aagggaggat tgctcctccc ctgtgactga
    14401 ctgttaataa gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat
    14461 cataaccctt ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc
    14521 tgcccagcta tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct
    14581 tttcttcgag cgtgctcgaa aaaccccatg caaaacagag ataatttttg ggaaaaagga
    14641 tctattacgg cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga
    14701 tgcaccccgc atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg
    14761 gtaagggagg atgactcctc ccctgagact gactgttaat aagctccgaa acttatcagt
    14821 aacagtacaa tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct
    14881 tcggtgaggg ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa
    14941 taaacacaac gcattgatct gactttgatt tattttctgg agcagactcg caaagtagaa
    15001 tgcgcaacgc ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc
    15061 cgttaatttt cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata
    15121 gctttcattg cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact
    15181 acataaccca tctgggcgga agtttgtaaa tattgcgcgg cgatgttgac gccctgctcg
    15241 gcgaagattt tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga
    15301 cgacgaccac catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag
    15361 ccattgtcag aatacttgat caatttaccc gcaacttcca ggccgatatt ctcctgcgct
    15421 tcctgagtcg aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagcgga
    15481 gaggtaaatg gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc
    15541 gccagatgtt tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc
    15601 gaagcattaa tcagcagcga gccgggcttc attagtgaaa tttctttcgc gcccatcata
    15661 tttttggtgg acggattctc tggtacatgc agactcacca catcgctcat attcagcagg
    15721 tcagaaagat gctgtacctg agtggcgttg cccagcggca gtttattttc aatatcataa
    15781 aagtaaacat acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg
    15841 tagccgatga tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc
    15901 cacacgccac ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt
    15961 tcgccaatca ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg
    16021 atcccgcgct ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca
    16081 atagcgacca gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc
    16141 aggccgatga agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct
    16201 ttgtgaaatt cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc
    16261 acgccttcta ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac
    16321 ccaatcctgt cttttgaaat gttgtgtgcg gatttgcgtc cgcctttcaa catatcaaaa
    16381 aataatattg cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg
    16441 taattcttgg atgaatatgc tggtttagaa gatttattta gagcaatcga caatagccag
    16501 gtaaaaacgt gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt
    16561 cccccgtcag atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg
    16621 tccgcgccac ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg
    16681 ttttccatcg ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgcca
    16741 ttatcggtca ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc
    16801 gccactgcac tacgtgccat cgggataact tctactggca gcgggaattt acccagaata
    16861 tcaacctgct tggaagcgtc tgcaatacag ataaattttt ctgcaaccga agcaatgatt
    16921 ttttcacggg tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca
    16981 tctgcgccat caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg
    17041 ccgaggcttt tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg
    17101 cctttcattg taccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca
    17161 acaatggtgc cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca
    17221 tcctgcgtca tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa
    17281 agcgattcag accccgttgg caagccgcgt ggttaactca tccataaaat atcgcgcaat
    17341 ggcgggcatc ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca
    17401 cggtgcgaac agaggcaata acgccacgcc attcaacaca accagggcgc gaactcgctg
    17461 agcgaaatgc attagcgcaa atccctgatc ttttaagctg cgcgtttaac cacgaataca
    17521 aatgttaccc gccgacggct tcggtatatg caacctgaca caaaattgtg tcatagtgca
    17581 ggaaaaagca tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta
    17641 caggcactgg atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc
    17701 attacacaat cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg
    17761 ctgttggtgc gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gctggcactg
    17821 ctgcgccagg tggagttgct ggaagaagag tggctgggcg atgaacaaac cggttcgact
    17881 ccgctgctgc tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttcctgca
    17941 ctggctcctg tgttggctga ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc
    18001 cgcactcagg aacgtctgcg ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag
    18061 gcgctgccga gttgtcttgt cgataaactt ggtgcgctcg actatctgtt cgtcagctca
    18121 aaaccctttg ccgaaaaata tttccctaac ggcgtaacgc gttcggcatt actgaaagcg
    18181 ccagtggtcg cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaacttc
    18241 gatctgcctc caggcagcgt gccctgccat atcgttaatt cttcagaagc gttcgtacaa
    18301 cttgctcgcc agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaagagctg
    18361 gccagcggtg aactgattga cttaacgcct gggctatttc aacgacggat gctctactgg
    18421 caccgctttg ctcctgaaag ccgcatgatg cgtaaagtca ctgatgcgtt actcgattat
    18481 ggtcacaaag tccttcgtca ggattaatcc atcaaataat gcctgatagc acatatcagg
    18541 cgttgtcctc acttcttttt gtattccttg aatcacatca caaaatagac aaatctcagg
    18601 cggcaaaaaa cgacgtctga atgcattttt tttgctggcg acaaacccac gtaaaaagct
    18661 caccgtaggc gcaaataccc tcattttgat tgcgttttac ggagcaaata atgtctaacg
    18721 tgcaggagtg gcaacagctt gccaacaagg aattgagccg tcgggagaaa actgtcgact
    18781 cgctggttca gcaaaccgcg gaagggatcg ccatcaagcc gctgtatacc gaagccgatc
    18841 tcgataatct ggaggtgaca ggtacccttc ctggtttgcc gccctacgtt cgtggcccgc
    18901 gtgccactat gtataccgcc caaccgtgga ccatccgtca gtatgctggt ttttcaacag
    18961 caaaagagtc caacgctttt tatcgccgta acctggccgc cgggcaaaaa ggtctttccg
    19021 ttgcgtttga ccttgccacc caccgtggct acgactccga taacccgcgc gtggcgggcg
    19081 acgtcggcaa agcgggcgtc gctatcgaca ccgtggaaga tatgaaagtc ctgttcgacc
    19141 agatcccgct ggataaaatg tcggtttcga tgaccatgaa tggcgcagtg ctaccagtac
    19201 tggcgtttta tatcgtcgcc gcagaagagc aaggtgttac acctgataaa ctgaccggca
    19261 ccattcagaa cgatatcctc aaagagtacc tctgccgcaa cacctatatt tacccaccaa
    19321 aaccgtcaat gcgcattatc gccgacatca tcgcctggtg ttccggcaac atgccgcgat
    19381 ttaataccat cagtatcagc ggttaccaca tgggggaagc gggtgccaac tgcgtgcagc
    19441 aggtagcatt tacgctcgct gatgggattg agtacatcaa agcagcaatc tctgccggac
    19501 tgaaaattga tgacttcgct cctcgcctgt cgttcttctt cggtatcggc atggatctgt
    19561 ttatgaacgt cgccatgttg cgtgcggcac gttatttatg gagcgaagcg gtcagtggat
    19621 ttggcgcaca ggatccgaaa tcactggcgc tgcgtaccca ctgccagacc tcaggctgga
    19681 gcctgactga acaggatccg tataacaacg ttatccgcac caccattgaa gcactggctg
    19741 cgacgctggg cggtactcag tcactgcata ccaacgcctt tgacgaagcg cttggtttgc
    19801 ctaccgattt ctcagcacgc attgcccgca acacccagat catcatccag gaagaatcag
    19861 aactctgccg caccgtcgat ccactggccg gatcctatta cattgagtcg ctgaccgatc
    19921 aaatcgtcaa acaagccaga gctattatcc aacagatcga cgaagccggt ggcatggcga
    19981 aagcgatcga agcaggtctg ccaaaacgaa tgatcgaaga ggcctcagcg cgcgaacagt
    20041 cgctgatcga ccagggcaag cgtgtcatcg ttggtgtcaa caagtacaaa ctggatcacg
    20101 aagacgaaac cgatgtactt gagatcgaca acgtgatggt gcgtaacgag caaattgctt
    20161 cgctggaacg cattcgcgcc acccgtgatg atgccgccgt aaccgccgcg ttgaacgccc
    20221 tgactcacgc cgcacagcat aacgaaaacc tgctggctgc cgctgttaat gccgctcgcg
    20281 ttcgcgccac cctgggtgaa atttccgatg cgctggaagt cgctttcgac cgttatctgg
    20341 tgccaagcca gtgtgttacc ggcgtgattg cgcaaagcta tcatcagtct gagaaatcgg
    20401 cctccgagtt cgatgccatt gttgcgcaaa cggagcagtt ccttgccgac aatggtcgtc
    20461 gcccgcgcat tctgatcgct aagatgggcc aggatggaca cgatcgcggc gcgaaagtga
    20521 tcgccagcgc ctattccgat ctcggtttcg acgtagattt aagcccgatg ttctctacac
    20581 ctgaagagat cgcccgcctg gccgtagaaa acgacgttca cgtagtgggc gcatcctcac
    20641 tggctgccgg tcataaaacg ctgatccctg aactggtcga agcgctgaaa aaatggggac
    20701 gcgaagatat ctgcgtggtc gcgggtggcg tcattccgcc gcaggattac gccttcctgc
    20761 aagagcgcgg cgtggcggcg atttatggtc caggtacacc tatgctcgac agtgtgcgcg
    20821 acgtactgaa tctgataagc cagcatcatg attaatgaag ccacgctggc agaaagtatt
    20881 cgccgcttac gtcagggtga gcgtgccaca ctcgcccagg ccatgacgct ggtggaaagc
    20941 cgtcacccgc gtcatcaggc actaagtacg cagctgcttg atgccattat gccgtactgc
    21001 ggtaacaccc tgcgactggg cgttaccggc acccccggcg cggggaaaag tacctttctt
    21061 gaggcctttg gcatgttgtt gattcgagag ggattaaagg tcgcggttat tgcggtcgat
    21121 cccagcagcc cggtcactgg cagtagcatt ctcggggata aaacccgcat gaatgacctg
    21181 gcgcgtgccg aagcggcgtt tattcgcccg gtaccatcct ccggtcatct gggcggtgcc
    21241 agtcagcgag cgcgggaatt aatgctgtta tgcgaagcag cgggttatga cgtagtgatt
    21301 gtcgaaacgg ttggcgtcgg gcagtcggaa acagaagtcg cccgcatggt ggactgtttt
    21361 atctcgttgc aaattgccgg tggcggcgat gatctgcagg gcattaaaaa agggctgatg
    21421 gaagtggctg atctgatcgt tatcaacaaa gacgatggcg ataaccatac caatgtcgcc
    21481 attgcccggc atatgtacga gagtgccctg catattctgc gacgtaaata cgacgaatgg
    21541 cagccacggg ttctgacttg tagcgcactg gaaaaacgtg gaatcgatga gatctggcac
    21601 gccatcatcg acttcaaaac cgcgctaact gccagtggtc gtttacaaca agtgcggcaa
    21661 caacaatcgg tggaatggct gcgtaagcag accgaagaag aagtactgaa tcacctgttc
    21721 gcgaatgaag atttcgatcg ctattaccgc cagacgcttt tagcggtcaa aaacaatacg
    21781 ctctcaccgc gcaccggcct gcggcagctc agtgaattta tccagacgca atattttgat
    21841 taaaggaatt tttatgtctt atcagtatgt gaacgttgcc actatcaaca aagtggcggt
    21901 cattgagttt aactatggcc gaaaacttaa tgccttaagt aaagtcttta ttgatgatct
    21961 tatgcaggcg ttaagcgatc tcaaccggcc ggaaattcgc tgtatcattt tgcgcgcgcc
    22021 gagtggatcc aaagtcttct ccgcaggtca cgatattcac gaactgccgt ctggcggtcg
    22081 cgatccgctc tcctatgatg atccattgcg tcaaatcacc cgcatgatcc aaaaattccc
    22141 gaaaccgatt atttcgatgg tggaaggtag tgtttggggt ggcgcatttg aaatgatcat
    22201 gagttccgat ctgattatcg ccgccagtac ctcaaccttc tcaatgacgc ctgtaaacct
    22261 cggcgtcccg tataacctgg tcggcattca caacctgacc cgcgacgcgg gcttccacat
    22321 tgtcaaagag ctgattttta ccgcttcgcc aatcaccgcc cagcgcgcgc tggctgtcgg
    22381 catcctcaac catgttgtgg aagtggaaga actggaagat ttcaccttac aaatggcgca
    22441 ccacatctct gagaaagcgc cgttagccat tgccgttatc aaagaagagc tgcgtgtact
    22501 gggcgaagca cacaccatga actccgatga atttgaacgt attcagggga tgcgccgcgc
    22561 ggtttatgac agcgaagatt accaggaagg gatgaacgct ttcctcgaaa aacgtaaacc
    22621 taatttcgtt ggtcattaat ccctgcgaac gaaggagtaa aaatggaaac tcagtggaca
    22681 aggatgaccg ccgatgaagc ggcagaaatt atccagcata acgacatggt ggcatttagc
    22741 ggctttaccc cagcgggttc gccgaaagcc ctacccaccg cgattgcccg cagagctaac
    22801 gaacagcatg aggccaaaaa gccgtatcaa attcgcctgc tgacgggggc gtcaatcagc
    22861 gccgccgctg acgatgtact ttctgacgcc gatgctgttt cctggcgtgc accatatcaa
    22921 acatcgtccg gtttacgtaa aaagatcaat cagggcgcgg tgagtttcgt tgacctgcat
    22981 ttgagcgaag tggcgcaaat ggtcaattac ggtttcttcg gcgaaattga tgttgccgtc
    23041 attgaagcat cggcactggc accggatggt cgagtctggt taaccagcgg gatcggtaat
    23101 gcgccgacct ggctgctgcg ggcgaagaaa gtgatcattg aacttaatca ctatcacgat
    23161 ccgcgcgttg cagaactggc ggatattgtg atccctggcg cgccaccgcg gcgcaatagc
    23221 gtgtcgatct tccatgcaat ggatcgcgtc ggtacccgct atgtgcaaat cgatccgaaa
    23281 aagattgtcg ccgtcgtgga aaccaacttg cccgacgccg gtaatatgct ggataagcaa
    23341 aatcccatgt gccagcagat tgccgataac gtggtcacgt tcttattgca ggaaatggcg
    23401 catgggcgta ttccgccgga atttctgccg ctgcaaagtg gcgtgggcaa tatcaataat
    23461 gcggtaatgg cgcgtctggg ggaaaacccg gaaattcctc cgtttatgat gtattcggaa
    23521 gtgctacacg aatcggtggt gcatttactg gaaaccggca aaatcagcgg ggccagcgcc
    23581 tccagcctga caatctcggc cgattccctg cgcaagattt acgacaatat ggattacttt
    23641 gccagccgca ttgtgttgcg tccgcaggag atttccaata acccggaaat catccgtcgt
    23701 ctgggcgtca tcgctctgaa cgtcggcctg gagtttgata tttacgggca tgccaactca
    23761 acgcacgtag ccggggtcga tctgatgaac ggcatcggcg gcagcggtga ttttgaacgc
    23821 aacgcgtatc tgtcgatctt tatggccccg tcgattgcta aagaaggcaa gatctcaacc
    23881 gtcgtgccaa tgtgcagcca tgttgatcac agcgaacaca gcgtcaaagt gatcatcacc
    23941 gaacaaggga tcgccgatct gcgcggtctt tccccgcttc aacgcgcccg cactatcatt
    24001 gataattgtg cacatcctat gtatcgggat tatctgcatc gctatctgga aaatgcgcct
    24061 ggcggacata ttcaccacga tcttagccac gtcttcgact tacaccgtaa tttaattgca
    24121 accggctcga tgctgggtta atgactcgtc ccgtcacgat cttcagcaat atgctgaagc
    24181 atcgtggcgg tataacgatg atttctgatt gaataaagat attcctgcat atacatcggc
    24241 ctgtgcggcg catcaaaata tttcagcgtt gccggattca ccagtcgcca ggcaaaatgc
    24301 gggatcaccg tcagaaaatg tccgcgctcc acggcgctga ttttggccat aaaactgtag
    24361 ggacgataaa taatggttgg attgatccca caaggtcgca tattcgcatc aatcattgct
    24421 tcgaagttgg cccggttctg aaagcgcatt tgtagccagg gcagctcctg aagcagctct
    24481 tgctcttgac gctcttcata acggcgagaa acgagaaagc ctaatcgcag cggtggtagt
    24541 tcactgatag tcaggttttc cagttcctga actcgtgcag atacatgctg tggggagata
    24601 ataaaatcta actggcggtc aaaaagatta tcgataacgc cgttttcgct gaactcaact
    24661 ggctgggcgg tcacgccttc atacttatct ccgagactaa tcaactgatc gaaaataatt
    24721 gtcggataag tattatctat gccaatcaca atttctcgtg aacgacgtcc tgagttatgg
    24781 atttcattat caatcgcaga aagccgttga taaaccggga acagtttttg atacagttcc
    24841 tgtccggctt tattgaggct gatattatta tctttgcggg taaatagcgt atagccaatt
    24901 tgttcttcca gcgcggcaat gcttttacca aatggcgaag ccgtcatatg ggttttttct
    24961 gccgcccggg caatattatt ggtttgcgct aataaaatga aattgcgcat ctttttcgaa
    25021 ataaaaatgt ccatccccct cccccgcatc cttgtccgct taaccgtttc agtcagccta
    25081 tccttgatga aaccgcgagc aaagataggt gattacgtca tggttttaca gaaaattaca
    25141 gaaaaaggag gcaatatcgg gtaaaggcat tagcccgacg aatacgtcgg gctacaagta
    25201 ttattgtgct gcaggtgttt tagcgggttg ttgatccaca ggttctaact ggaagaccac
    25261 atcgacctga tcatcaaact gaatagcggc ctgctcgtaa gtttcctggg cggacaccgg
    25321 cgcggcatcg gctttcatca tccgcaccat tgggctgggc tgatagttgg aaacatggta
    25381 gcgcacgcta tataccggcc ccagtttacg atgaaagccg ttcgccagtt cctgcgcctg
    25441 atgaatcgcg ttatcaatcg ctgccttacg cgctttgtct ttataggcat ccggctgcgc
    25501 cacgcccagc gacacagaac gaatttcgtt aagacccgcc ttcagcgcgc catccagcaa
    25561 ggaattcagt ttgtctaact gacggagcgt gacttccacc gttctcacag cgcggtagcc
    25621 tttcaggata cttttaccat cctgataatc ataatctggc tgggtgcgta agttcgctga
    25681 gctgatatct tttttcgcga tctgattgag ttcaaggaag gaaatgtatt gtgcgacgcg
    25741 ctcatctgcc tgtttcttgg cagtagcggc atccttcgcg gccacgttaa cttcaatcgc
    25801 aagagtggca atgtctggca ccgcatccac gcttgccgta ccggaggtga caatatgcgg
    25861 tccatccggc aattcgttag cctgcgctgc catcccgcta atacccatta atgccgccag
    25921 ggcgataact ttgaacttca cgttagtctc tccgtgttaa ttgacttgcc caaaattcag
    25981 gacctctgga ggcaagctta gcgcctctat tttatttttc catcagatag cgcctaactg
    26041 aacaaggctt gtgcatgagc aataccgtct ctcgccagct gcaaggctat aaaccacatg
    26101 acacatccca ctaccagatt gataatgcgc tgtgattttg ccgtgcgcag gcgcggtgcc
    26161 agccaggctg cgagaatagc cagaccaaag aaccacagga aagaggcgct aattgtcccg
    26221 agcgcaaacc aacgttttgg ttcgacgtca agctgcccgc caaggctgcc cagtacaaca
    26281 aaagtatcca ggtaaacatg cggattcagc caggtcactg ccaacatggt ggcgataatt
    26341 ttccatctgc cttgcttcag gacttcggca ctggctaact caatattact gctcattgcg
    26401 gttttaaaag cgccaaaacc ataccacagc aagaaggcta cgccgcccca ggtgaccagc
    26461 gccagcaacc acggcgactg catcaataac gcgctgccac caaaaatccc ggcgcaaatc
    26521 aggaccaaat cgctgatagc acaaagtaag gcaatcataa tgtggtactg acgacgaatg
    26581 ccctgattca tcacaaaagc attttgtgga ccgagcggta ggatcatagc tgcgccaagc
    26641 gcaagacctt gaaagtaata agaaaacacg ttggatattc cgaattaagt atctgatgca
    26701 gagactatat cgcgggattg ttattagcgg aaattgataa tattaattgg caatcagaaa
    26761 aactaataag agagaggcca gagagtatta tctggcctca gcgttgatta cgcagctttg
    26821 tcttctttca cccgcttaaa gttcacatcc atttgcgggt acgggaagct gataccggcg
    26881 gcatcaaatt cacgtttaat acgctccagc acatcccagt acacgttttg cagatcgccg
    26941 ctgttgctcc agacgcggac cacgaaatta atcgacgatg caccaagttc gttcaggcgc
    27001 acagtcattt cgcgatcttt caaaatgcga tcttcagact ggataatatt ggtcaggatc
    27061 tgcttaacct gatcgatatc ggaatcatac gccacgccaa taataaattc gttacggcga
    27121 actggctcgc gggagaagtt aataatattt ccggcaataa ttttaccgtt cggaataacg
    27181 ataattttac cgtctgcagt acgcatggtg gtggagaaaa tctgcacact cagcacagta
    27241 ccggctacgc cgccgaggtc aacatattct ccggcacgga acgggcggaa catgacaagt
    27301 aacacgccag cggccaggtt agaaagtgac ccctgcaaag ccagaccaac agctaagcct
    27361 gcggcaccga gtacagcaat gactgacgct gtttgtacac ccacgcgtcc cagggcagcg
    27421 attagcgtaa aggcgataat accgtaacgg actaatgcag aaagaaaatc agcaacagtg
    27481 gcatcgattt tacgggagat catcaggcga ttcaccgcgt tggaaatcat ccgcgcgata
    27541 atcaaaccaa cgatgatgat cgcgagtgcc gccacgatgt ttactgcata acttagcagc
    27601 agcgcctggt tagctaccag ccagcttccc gcgccgttta tgctatcgac aacattcaaa
    27661 tcttccattc aatattcctt ttcaactgac tcaaaatgga gaaatacact ccgccgcctt
    27721 atgacgggca gtctgacaag ggtaaacaat aaagcataat tttgccaaat agatcacaga
    27781 tttctcatct gaatcacctt gcttttggcg aaaaaatccc cgcagagcgg ggctcagaca
    27841 gatatcaatg tgcgctttgt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    27901 aacctgcaaa ttcaatatat tgcaggatac gtgtaggcct gataagcgta gcgcatcagg
    27961 caattttgct tttggcgaaa aaaagacccg cagagcgggc cttgagataa gcagaaagga
    28021 atatcttaca gaacgtcgat cgcgttcagt tcctggaatg ctttttccag acgagcgatc
    28081 atcgaagtct gaccggcacg cagccatacg cgcggatcgt agtatttctt gttcggctga
    28141 tcttcgcctt tcgggttacc cagctgaccc tgcagataag cttcgttcgc tttgtagtag
    28201 ttcagaacgc cttcccaggt tgcccattgg gtatcggtat cgatgttcat ttttactacg
    28261 ccgtagctta cggagtcttt gatttcctga gcagtagaac cggaaccacc gtggaatacg
    28321 aagttcaggc tgttgtgcgg caggttgtgt ttcttggaaa catattcctg agaatcacgc
    28381 aggatggtcg gagtcagaac cacgttaccc ggcttgtaaa caccgtgtac gttaccgaag
    28441 gacgctgcga tggtgaaacg cgggctgatt ttgctcagtt cggtgtatgc gtaatcaacg
    28501 tcttccggct gggtgtacag tgcagaagcg tccatgtggc tgttgtccac gccgtcttct
    28561 tcaccaccgg tgcaacccag ttcgatttcc agagtcatgc cgattttgga catacgctcc
    28621 aggtatttag agcagatttc gatgttctct tgcagagatt cttcagacag gtcgatcatg
    28681 tgagaagaga acagcggctt accggtagct gcgaagtgtt tttcacccgc gtccagcaga
    28741 ccgtcgatcc acggcagcag tttcttcgcg cagtggtcag tgtgcaggat aaccggaaca
    28801 ccataatgtt cagccatctg gtgaacgtga tgcgcaccag agatcgcgcc caggatagca
    28861 gcaccctgcg gaacgtcaga tttcacgcct ttaccagcga taaaggaagc accaccgttg
    28921 gagaactgaa cgataactgg cgctttaact ttagcagcag tttccagtac ggcgttgatg
    28981 gagtcagtac cgacgcagtt tactgctggc agtgcgaagt tgttttcttt tgctacctgg
    29041 aaaactttct gtacgtcatc accagtgatt acgccaggtt ttacgaaatc aaaaatctta
    29101 gacatgtctc ttgtcctgta tcgtcgggcc ttatactcat catacttcaa gttgcatgtg
    29161 ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg gattcactct
    29221 cttgtcgcct tcctgcaact cgaattattt agagtatgaa aaaatgcgtg ctctaaaagc
    29281 gcgctgaaac aagggcaggt ttccctgccc tgtgattttt tacttcttag cgcgctcttc
    29341 gagcatcgct actgcaggca gtactttacc ttccacgaat tcgaggaatg cgccgccgcc
    29401 agtggagatg taggagattt tgtcagcaat gccgaacagg tcgattgctg ccagagtgtc
    29461 gccgccgcca gcgatggaga acgcttcgct gtctgcgata gcgttagcca cgatttcagt
    29521 acctttgcgg aagttcggga attcgaacac gccaaccgga ccgttccaca gaatggtttt
    29581 cgcattcttc aggatttcag ccagttcctg agcggaagca tcaccgatat ccaggatctg
    29641 ctcgtcagct ttcacatcgt taacagattt cagggtagcc ggtgcagttt cagagaactc
    29701 ggttgctacg cgaacatcag acggaaccgg gatgttgcag gtggtcagca gacgtttagc
    29761 ttcgtcaacc aggtcagctt cgtacaggga tttacccaca tcgtggcctt gtgccgcgat
    29821 aaaggtgtta gcgataccac caccaacaat cagctggtca gcgattttag acagggagtc
    29881 cagaacggtc agtttggtag atactttaga accaccaacg atagccacca tcgggcgagc
    29941 aggttctttc agtgctttac ccagcgcgtc cagttcagct gccagcagcg ggcctgcgca
    30001 cgcaacgtca gcgaatttac cgataccgtg agtagaagcc tgcgcgcggt gagcagtacc
    30061 gaatgcgtcc attacgaaca cgtcacacag tgcagcgtat tttttggaca gggtttcgtc
    30121 gtctttcttc tcgcctttgt tgaagcgaac gttttccaga acaaccagtt caccttcagc
    30181 aacgtcaacg ccgtcgaggt aatctttaac caggcgaacc gggttagaca gtttgtcttt
    30241 caggtagtta acaaccggca gcagagagaa ttcttcgttg tactcgcctt cggtaggacg
    30301 acccaggtgg gaagttacca tcacttttgc gccttgtttc agggccagtt cgatggtcgg
    30361 cagagaagca cggatacgcg cgtcgctggt tactttcccg tcttttactg gtacgttcag
    30421 atccgcacgg ataaatacac gtttcccagc aagatccaga tcggtcatct taattacaga
    30481 catggtgaat cctctcgttg attctaaaag ttttgcagac gctgcttgcg tcttacctga
    30541 aagcaacagt agccatagct aacgtcgtgt cgagcattcg gttagcaaag ccccattcgt
    30601 tatcgcacca gaccaacgtt ttgatcaggt gtgcgccact gacccgggtt tgggtgccat
    30661 cgacaatggc actgtgcgga tcgtggttaa aatctacaga gaccaacggc aattccgtat
    30721 agtcaactat accatgaaat gcaccttgtg ctgctttttg cagcaacagg ttgacttcat
    30781 tggcttttac aggtttcttc accgtcacgc ttaaatcgat tgccgtcaca tttatggttg
    30841 gcacacgtac cgcaatcgct tcaaagcgat cgttaaattg cggaaaaaat cgtgtgatac
    30901 cggcggccag tttagtatcg accggaatga tcgactggct ggctgcccgg gtgcgacgca
    30961 ggtcaggatg gtatgcatca ataacctgtt gatcgtgcat ggcggagtga attgtggtca
    31021 ctgtgccgga ctcaataccg tacgcatcat ctaacaattt gatgacggga attatgcaat
    31081 tcgtggtaca ggaagcgtta gaaacgatgc ggtgttccgc acgaagttga tcctgattga
    31141 cgccgtaaac aacggtcgcg tcgagatcgt tactgccagg atgtgaaaag agcacttttt
    31201 tggccccggc ggcaatatgc gcttcgccat gctcgcggga gccatatacg ccggtgcagt
    31261 cgaggactac atcaacgcca agttcacgcc aggggagcga ttgcagtgaa cgttcatgca
    31321 atacgcggat ggcgtcatca ccaacaaaaa gttgatcgcg ttcctgtcgt acttcccatg
    31381 caaaacggcc atggctggtg tcatatttca acaaatgcgc catgcccgca gcatccgcca
    31441 gttcgttgat tgccaccacg gtaatttccg cccggcgtcc ggattcatac aaagcacgaa
    31501 ccacattacg cccgatgcga ccgaagccat ttatcgctac gcgtacggtc atagatctcc
    31561 tgcaaggttt tccctgagca aatttgccag acagagtaat ccagcaaatc gtccggggaa
    31621 accttacctg tcgcaaactg cgactgattg gttaattgtc gaacatttaa tcgactgaaa
    31681 cgcttcagct aggataagcg aaacgtggaa taaaaggaat gattgtccag tcaaagaaaa
    31741 caaatatctg acttacgtca cgttttcagc cccgcccctg tggaatttat tccactttgc
    31801 agaatcacga ataagcgtag caaaatcttt tcgttgtgcc gtcacaaacc ctgacttata
    31861 tcagaatatg actgatgcag tgtcgatatt atccgcaata cgtccgagtt tcggagtact
    31921 ccaatgatga cattgcccta cccatattct tcctccgcac gcttttacgc actgcgcctg
    31981 ctccccgggc aggaagtttt ttcccagcta cacgcttttg cacagcagca gcaacttcat
    32041 gccgcctgga ttgcaggctg taccggtagc ctgactgacg tcgccttgcg ctatgccggg
    32101 caagaaggca ccacgctcct gaacggtaca ttcgaagtga tctcactaaa cggcacgctg
    32161 gaacagagtg gtgagcatct tcatctctgc gtttccgatc cacacggcac gatgcttggc
    32221 ggtcatatga tgcccggctg taccgtgcgg accacgcttg aactggtgat cggctgcctt
    32281 gaggaactgg cgtttaaccg ccaaccttgc gcgctttctg gttacgacga gttgcatatt
    32341 tcccccgtta aataactact ttcaaagagg ttttcttatg gcgcgtagcc acttttccag
    32401 tcaggcactg gtgttgatcg ttatatctat cgccatcaat atgatcggcg ggcaactggc
    32461 cagcatggtc aaattaccta tatttctcga ctccatcggc acacttatta gcgcggtact
    32521 gcttggtccg gtgatcggaa tgctgaccgg cttactgacc aatttactct ggggattgct
    32581 gaccgacccg atcgccgccg catttgcacc cgtcgcgatg gtcatcggcc tggtcgccgg
    32641 ttggctggcg cgagcgggtt ggtttcgcac cctgccaaaa gtcgttgtca gcggcgtgat
    32701 tattacgctt gcggtaacgg tcgtcgctgt accgctgcgt actgcgctgt tcggcggtgt
    32761 caccggtagc ggagccgatt tgttcgttgc ctggatgcac tcgatggggc aaaacctggt
    32821 ggagtcggtg gcgattacgg tgataggcgc gaacctggtc gataaaattc ttacagcggt
    32881 gattgtctgg cttttactgc gccagctgcc gattcgcacc acgcgccatt ttccggcaat
    32941 ggctgccgtg cgctaaatgc atccatttac ctcattaaca ctgtgggcat tggcggcctg
    33001 caccacgctt atcctgcccg cgcagaccat tttgccgatc tatagcgcgg cgaccttttt
    33061 ctgccttatt gcgctaaagg ccacgcggcg gcgggcaaaa tatgtcgtct ggctgatgtt
    33121 ctcgctggga gccgggctgt ggctggtaca cggtggctgg ctaccagaat ggctaagcgg
    33181 cacgccacgc tcgccagaac ggtggtcgca tgctattacg ctctggttgc ggattctggc
    33241 gattgtttca acctcacagt tgtggatgca gtacgtcccc gtgcaatgtt ttattcgcgc
    33301 tctgtttgct tctcgcctgc cgccaggcgt cgcttatctg tttgccgggc cgttattggt
    33361 cgttgagcag ttaaaacggc aattagcgat tattcatgaa gcgcagcgtg cccgcggcgt
    33421 gccgctggat gagggctggt atcagcgttt acgtgcgatg cccgcgttaa ttattccgct
    33481 aacacacaat gcgcttaacg atctggctgt ccgtggtgcg gcgctggata tgcgggcatt
    33541 tcggatccac aaccgacgaa caacgttgtg ggcaccagcc gatagccccc tacaacgcgt
    33601 agcgcgttac accatgatct tgctgatgct gacagaattc ggagcgtgga tatggttacg
    33661 ttagaacagt tccgttattg cccgacacac tcaacgcatc ctccgttctg ttatgacttt
    33721 cactatgtca aaccggggat ggtcgccatc tttggtgaca acggcagtgg caaaagtacg
    33781 ctggcacagc tgatggcggg ctggtatccg gactttctgc caggagagat aacaggcacg
    33841 gggacgctcc tcggtacgcc cattgggcat ctcccgctta atgaacaatc cgccaccatc
    33901 cagctcgtcc agcaatcgcc ctatttgcag ctctccggct gtacctttag cgtggaagaa
    33961 gaagttgcct ttgggccaga aaatctgtgt cttgctgaag cagagattat ggcgcggatt
    34021 gatgccgccc tgactctgac cgagtgccag ccgttgcgcc atcgtcaccc ggcaacactc
    34081 tccggtggag agacacagcg cgtggtcatc gcctgcgcca tcgcgatgca gccaaagctc
    34141 ttaattcttg atgaagcctt tagccgcctg actccgcagg ccagcgaaat gctgttgcag
    34201 cgcttacaac actgggcgtt tgaacgtggt tcgctgatta ttctttttga acgtcatcgc
    34261 acacctttcc tgaactactg ccagcaagcg tggcaattgc aaaacggagc gctccagcca
    34321 ctatgttaac gttaaataag atatcttatc gctggcctgg cgcggcaacg gattgtcttt
    34381 gcgacatttc tctgcaactc aaacaaggtg aatggttggc gctgaccggc gacaatggcg
    34441 cgggaaaatc gacgctgtta cgagtaatgg caggtcttct tacgcccaca gccggtacgg
    34501 ttatgctgca acaacaggcg atgaaaaacc tgaaaaaccg ccagcgcgcg gcaaaggttg
    34561 gcgtactgtt tcaggaagcg gaaaaccaac tttttcacag caccgtcgct gacgagatcg
    34621 catttggttt gaaactgcaa aaatgcccgg cggatgaaat cactcaacgt acccatgctg
    34681 ccctgcaatg ttgtcagtta gcagacactg ccaacgcgca cccgttggat ctgcatagtg
    34741 cgcagcgacg gatggtcgcc gtcgcctgcc tggaagcact ctctccgcca ctattgctgc
    34801 tggacgagcc gagtcgggat tttgacgaaa attggttgag cgtgtttgaa agctggctgg
    34861 agaagtgccg ccagcgaggc acgagcgtcg tagcaatcag tcatgacgcc gcatttaccc
    34921 ggcgtcattt ctctcgtgtg gtgcgactgg aagatggtct aatcaggaac gttaatccac
    34981 cagacgatat tcacccgtag cggtcatcat taaggtcaaa tttgcaggca gactctcttc
    35041 cagcacccgg cgaacattcg gcccgtcggt acggtcataa aaagcttctg cgtctgccag
    35101 cgatacccct cccgccagct tgcgccctac cagtcgttcc cgcaacgcag tagctggcgc
    35161 gttgataaag atcgagaaat cacaaaattg cgcaagctgg caccacttct tgtcgtccag
    35221 caacaaccag tttccttcga cgatgacgag tggtgcggta acgtgcaacg catcttcaac
    35281 aggatcatgc ttttgtcgat cgtactgcgg ccacgtacaa tctccttcca cgacctggcg
    35341 cagattttcc gccagtttca caacgtcaaa tgtctctggt gcgcctttga aggggcgcaa
    35401 ttgatgcgcg tccagccagc tattgtaatg gtgaaaacca tccatcggga gcgtctgaat
    35461 agcgggcagt tccgggtctt gttgcgcgag atattcccag aaggtagtta aggtagattt
    35521 acccgtcccc ggcggagcgc aaagaaaaac caccgtacgt cgctgcgaac ttaccgcctg
    35581 taaggccgcc agcatacgca gtagcggttt atgaacattc tcaatttcat catcagagta
    35641 ctgagcctgt acctttagtc cattcaccgt cagttcgata ttcacggcgt taattccttt
    35701 aaagttgtcg ttactgccag cgtcagcgcc gttttgacca ttccgctaaa acgcgcctct
    35761 gaccagacgg cgaaatcggc gaacttcatc gctttcaacg cgtcatagag cgtcgcattg
    35821 caggttatgc tattgacgtt gctgataaaa tcccaggtca tgtcatcggc aaatccgggc
    35881 gtttcatcct gttcgttcag gtaatgccag aactgggaaa aatgcgtgat atcggcgtac
    35941 agccatttat ccattttccc cagcgcataa atcaaacgca gtgcaacatc aatatcgtca
    36001 agcgggccgc tttgggcgag taacggcttc actgcatatt cgacgatctc ttcatcgtta
    36061 tcgacaaaca gcgacggcag aaactggcgt atcccctgga gcaaaataga atgcgcagtt
    36121 ttcataaatg aaaataaatt gtcctgtgca tccagttgct caagcacatc atcttctgtc
    36181 agcgtcgcca ttggtcttcc gatagatcac catcaatgca gggagtctat tatattacac
    36241 gtttggtccg cgccgccgat cagtaatgtc tgcgtccgca ccccattcgc cgtctgttgg
    36301 ataccgttca ccaactcgcc gcccgtcacg cccgtagcgc taaagaggat atcgttcccc
    36361 ctcaccagtt catcgagcga gtacacacgg ttaacgtcaa cgcccattgc cttacaacgc
    36421 ttgcgctcct gctctgcaat ctgccgattt tccgtgtaat cccctttagc ctgacaaaag
    36481 tcaatcagct cggcctgcat atccccacct aatgctttaa cggcacaggc agaaatcacg
    36541 ccttctggcg caccgccgat ggtgtacatc acgtcgtaag gattatcctg ccagcaggtc
    36601 agcacgctgg cggcgacatc gccatccggc agagcgaaaa ccttcacgcc cagctgtgtt
    36661 gcttcttcaa tcgcggcact caggcgcggt ttatccagcg taaccatgcg cagcttgtcc
    36721 agcggtttgc ctaacgcccg cgccacgttg cgcaagttgt ccgccagagg caaagacaga
    36781 tcaatcgccc cggcagccag tcgattaaca accagctttt tcatatacat atcgggcgca
    36841 tgtaacaggc tatctcgagg ggcgaaagcc atgaccgcca gcgcattgct ctgccccatc
    36901 gctaccatcc gcgtgccttc aatcgggtca accgcgatat cgacttctgg cccatcgcct
    36961 ttgcccacct cttcgccaat ccacagcatc ggcgcatggt caatttctcc ttcgccgata
    37021 accactcgtc cacgaaaagc gacgtcgttt aatgcctggc gcatcgcggt gaccgccagg
    37081 ccatcaattt tatttttgtc gccacatccg gtttgcggcc aggcagccaa cgctgcctgt
    37141 tccgtaacgc gaaataatgg ccacgccagg gacatcattg cgcggcctcc ccaatatcaa
    37201 cgccaaattg cgccagcaga tatttctcgg cctgctcatt ccagatgccg tgcgtttcct
    37261 caaccagccg cgccagctct ttaaacagcg gatcggtttt gcctttttcg gcaaaatcgg
    37321 caatggcggt gagcggcatg gtcacgccgt tataaatgag ttttttaccg ccagggatat
    37381 ccggcagatt gagcacggta tctggcaccg catccagccc accgatatgc gtgaccataa
    37441 aggacggctg caactgcccc gtggcgctta acgcaatcgc ctctttcatg tcgtccgttg
    37501 aaccgccgga tgtaccgact acgtgcgtgc tgttgtaatg gacgttgtag aaattaaacg
    37561 gcactttgaa gtttttatcc gtcggcccgg caaagaagtt cagacagcca tcttccgcca
    37621 gtaattcgtc agccatctca acgacagcag gcaccgccgc ataaacaaaa atgtcatcga
    37681 acccgacatc tcctgtcagc gcccgcagcg tctggaccgg atcgctcatc cctttggtat
    37741 tcacatacac cagctcaatg cctttactgg ccgccagatc caccggcagc agcttctgca
    37801 cctgcgccag gcgtttgtcg tcgatatcga ccaccaccac ccgtgacggt tgtatgccac
    37861 cgttaatggc gtaatcgata gcgccaatgc ccatcggacc cgcacacgcc agcagtgcaa
    37921 tattgccgcc aggtttgacg cccatgcgat gctcataaac atattgcgtg gtgtgataat
    37981 tggcatgata agcaccaata atgcagcaca taggctctgc cagcgacgcc gcagcaaaat
    38041 aagagccgtg atacggtaat acgcagccca aattaatggc gatttcggga ataatcatat
    38101 aggtggcgtt accgccaaaa tattcgtagc tgtagcctgc tgaatatccg cttggtaacc
    38161 ccatcgccgg ttgcaataca aaacgctggc cttttttata tttgccagtg agatttttac
    38221 ccacttcgac aataaccccg gcacattcat gcccggtaat gaccggatga ttttctaaat
    38281 cgtcgggtac gcgtttatgt tcactaccga gtaacgccgc tttccaggtc gataaacaga
    38341 cgctgtcaga aattacactc actaacagtt cattatcggt aatttctggc agttcaaatt
    38401 cgcgcagacg gacatcccgc ttgccataaa tagcagcaac tttggttttc atttttacct
    38461 ctgtaatcag tgtttatgtt cggcggttaa ttgattaaat aaagtgtcga gttttggatc
    38521 gccaatatag ttattaatca atatcagcgg tttatccgtc acacgtttca cgcgcccttc
    38581 cagactggcg tgagtgacga cgatatcggc atccgcaggc acgttttcta tcgcgtaatg
    38641 ttttacttct attgccagcc ccgctttttc caggcgttta cggaaggtgg tcgcgcccat
    38701 cgcactggag cccatccccg catcgcaaac aaaggcgata cgcttaaccc tgcttaacga
    38761 gaatgcaccc tcttgtttca tcgccttaac cgcattggct gactgagcaa actcatcttc
    38821 gctctccgtt tccaccgttt tttccatctt cagtatcagc gaagtaatag caaaggacac
    38881 cagggtacct accgtaacac cggcaattgt cgccaggaac gagcctttcg gcgtcagtgc
    38941 caggtaagca aagatagacc ccggacttgg gccagccacc agaccaccgt ccagtaagtt
    39001 aaacatccag gtaccagaca taccgcccgc aatcatggca ataatagtca gcggcttcat
    39061 cagcacatat gggaaataca gttcgtggat cccaccgagg aagtgaataa tcatcgcccc
    39121 gggcgcagaa cgtttactca tccctttacc aaacaaggta aacgccagca gcagccccag
    39181 gcccggacct gggttagagg ccaccataaa gaagatggat ttaccgttaa ccgaagcctg
    39241 ttgcattccc agcggataat agacgccctg atcgatcgca ttattaagga aaagcacttt
    39301 cgccggttca ttgatgactg acagcagagg cagataaccc gcatgtacca gcgcctcgat
    39361 acactctttg acgaaagtat tggcaattaa caccgccggg ccgatcactt caaaacccag
    39421 cagacaaagg agcatccccg cgatacctaa tgagaagtta ttgataacca tctcaaaacc
    39481 ggcaggtatg cgtttttcca gtgccttatc gacatatttt atgaccaacc caccgagcgg
    39541 ccccatgatc attgagccaa ggaacatcgg gatctctgcg ccaacgatca caccaatagt
    39601 acctattccg cccatgaccg ccccgcgttt accgccgacc agatgaccac cagtagaacc
    39661 aatcatcacg ggcaataaat aggtaatcat cgggccgaca attttggcga aatgttcatt
    39721 cggcagccaa ccggtgggaa taaataacgc agttatgaag ccccaggcaa taaaagcacc
    39781 aatattgggg atgaccattg cagtcaaaaa gcccccaaaa gcctggacct ttgcacgagc
    39841 agacttgttt tccataatat tatcctgtag aggagagagt aattatccgc ggctgataat
    39901 gtccgctaat tgtttttctg atgatgctgt taagagttgt tcgagtattt cttcttcgca
    39961 cagtaattca cttaacgcct gaatagcgcc aatgtgcgaa tcggcatcgg cagcagagag
    40021 tccgatgagt aatttaattg gttcgtcatt ccccggaaaa taaacacctt gttcaagtaa
    40081 tgtcaaagac atcccggttt taagcgcccc acattccggt cgcgcatgag gcattgccac
    40141 gcctggtgcg agaatataat aagggccatt gttaatggtg gaatctttaa ttgcctgaat
    40201 gtaattctcg ctgatatagt ttttatccag caatgatacc atcgagaaat cgatagcttc
    40261 ctgccaatct tttgctgaat gtataactga gattgatgat tctggaaaat aatcactaag
    40321 ccgcataaat tatccttatt ttatttttag ggtaccgtcc ggcggtaaaa ataaaatact
    40381 cgtgcggttt aatgaaagca attgagcagg gaaaccgatg ttttgaaaac agtgaattat
    40441 taatttctta tataacattg agttatagat ataacaacaa atatcaatac gcatatcgtg
    40501 atgcatatta cataattgat atttattgca tataaatatt tgtgatctac aacacgcttt
    40561 atctattgct tgtccgcaaa cggacatatc aaggtaataa aaaaggtcgc cgaagcgacc
    40621 tttttacccg aaatgctaat tacagcagtt cttttgcttt cgcaacaacg ttatcaacag
    40681 tgaagccgaa ctcttcaaac agcagctctg ccggagcaga ttcaccgaag ttggtcatac
    40741 cgacgatagc accgttcagg ccaacatact tgtaccagta gtcagcaata cccgcttcta
    40801 cagcaacgcg tgcagtaacc gctttcggca gtacggattc acggtaagca gcatcctgct
    40861 tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc tttcacgcct tcggcagtca
    40921 gtttttcgta ggcagcaaca gccagttcaa cttctgaacc ggtagcgatg aaaatcagtt
    40981 ccggctgacc ggcgcagtct ttcagcacat aaccaccgcg cgcgatgttt gccagttgct
    41041 cttcagttcg ttcctgctgc gccaggttct gacgggagag gatcagtgcg gtcgggccgt
    41101 cctgacgctc aacaccgtat ttccacgcga ccgcggattc aacctggtca cacggacgcc
    41161 atgtagacat gttcggggtt acgcgcagag aagcgacctg ctcaaccggc tggtgagtcg
    41221 ggccgtcttc gcccagaccg atggagtcgt gggtgtaaac catcacctga cgctgtttca
    41281 tcagcgcagc catacgtacg gcgttacgtg cgtattccac gaacatcagg aaggtggagg
    41341 tgtacggcag gaaaccaccg tgcagggaga taccgttagc aatcgcggtc ataccgaact
    41401 cgcgaacacc gtagtggatg tagttacccg cagcatcttc gttgattgct ttagaaccag
    41461 accacagggt caggttagac ggtgccaggt cagcagaacc gccgaggaat tccggcaaca
    41521 gcggaccgaa agcttcgata gcattctgag acgctttacg gctggcgatt ttcgccgggt
    41581 tagcctgcag tttagcaatg aactcttttg ctttcgcgtc gaagtcagac ggcatttcgc
    41641 ctttcatacg gcgggtaaat tcagcggctt cctgcggata agctttcgcg taagcagcga
    41701 atttctcgtt ccatgcggat tctttcgcct ggcctgcttc tttcgcatcc cactgagcat
    41761 agatttcaga cgggatttcg aacggtgcat atttccagcc cagctgttcg cgggtcaggg
    41821 caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg
    41881 aaccgaaacc gatgatggtt ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg
    41941 cttcttctac tgcgcgtttg atggatgccg cgtcatgacc gtcgatgtcg cgaataacgt
    42001 gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt gaaccagcct tcaacgtgac
    42061 catcaataga gataccgttg tcatcgtaga acgcaatcag tttacccagc ttcagcgtac
    42121 ccgccagaga gcaaacttcg tgggagatgc cttccatcat gcagccgtcg cccatgaagg
    42181 cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt
    42241 tttctgcaat cgccatacct actgcgttgg caataccctg ccccagcgga ccggtggtgg
    42301 tttccacacc agcggtgtaa cccacttccg ggtggcccgg agttttagag tgcaactgac
    42361 ggaagttttt cagttcttcc atcggcagat cgtaaccggt gaggtgcagc aggctgtaga
    42421 tcagcatgga gccgtggccg ttggacagca cgaagcggtc acggtcagcc caggacggat
    42481 tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc ggcaatgtca gccataccca
    42541 taggggcacc cgggtgaccg gatttggctt tctgtactgc gtccatgctc agcgcacgaa
    42601 tagcattggc aagctcttta cgtgaggaca ttttgactcc agatcggatg atgaagggca
    42661 cgcccttaac gacttgacga cagcgcgttt tgggctacgc cggaaaattt gccaacaatt
    42721 taccgcaagc cgcgcgtcat gtacatggaa catccttttg ccgcttcaga aatctctgga
    42781 tcatgctcgc atgttgcgca atctactcgc ccgtccgctg cgcttttcct tatactgaga
    42841 ctgagcgtcg attcacctgc aaacggcgca tttttagaat aatcctgacc ttgtgcggaa
    42901 gagaaaacat gaaaattcgc gccttattgg tagcaatgag cgtggcaacg gtactgactg
    42961 gttgccagaa tatggactcc aacggactgc tctcatcagg agcggaagct tttcaggctt
    43021 acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca
    43081 gcaaggcgac gattgcgcca gccaatagcg aatacgctaa acgtctgaca actattgcca
    43141 atgcgctagg caacaatatc aacggtcagc cggtgaatta caaagtgtat atggcgaagg
    43201 atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt ctatagcggg ctgatggata
    43261 tgatgacgga taacgaagtc gaagcggtga tcggtcacga aatggggcac gtggcgttag
    43321 gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa tgccgtgcga gtagctgcgg
    43381 cctctgcggg cgggattgtc ggaagtttat ctcaatcaca acttggtgat ctgggcgaga
    43441 aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga agccgatgat tattcttacg
    43501 atcttctgcg ccaacgcggc atcagcccgg caggtcttgc caccagcttt gaaaaactgg
    43561 caaaactgga agaaggtcgc caaagctcaa tgtttgacga ccatcctgca tccgccgaac
    43621 gcgcccagca tattcgcgat cgtatgagcg cggatggggt taagtaagtc agacataccg
    43681 atctacaaca taattgtagg tcggtaacgc ctgaatcccc gatcagagta gtgatttcac
    43741 tttatttagc aaaacggcta catcatccgg tcgggtatat cccgtatcag ggtctataat
    43801 cgagctgtaa acatgtggca taatgcgcgg cacccccgcc tccagacaag ttttcagaat
    43861 cacactgaag ttatccaaat caatcccgcc agtcggctca atcagcgtca taccattgcg
    43921 cgccgccgtg gtcgccagcg cgtatagctc cggaagcgac ctctcgccgc ccatcggaaa
    43981 gaacttcgcc gcgtgtgcgc ccatatctag catcattcgc accgccgtat cgcaggagac
    44041 gcgggcaggc gtaccctggc tgctgctaac gccggtggaa atcagcactt cgcccggcgt
    44101 ccccgtcggg ctaaccagcg cgttgacatg ggtctgctca ccgcccgtag ccgcaagcgc
    44161 gcctgccgcg aaaccacaac cggtaaaggt ctggttaacg tgtgctggat gaatctgcgc
    44221 cgcaatcatc gcagctttgt aatactgcgc cgggtcaccc gcccccagac ctactgaaat
    44281 ggacggtatt tccgccatcc aacgtttaac ttcctggatc ccctcatcca ccgaggagaa
    44341 ctgcgcggaa agcacgccaa tcactgcgtg gccttcggcg gcgtcaaaga tttcacgggc
    44401 attggcgata tctttcgcca gcacattaat tgccacgcga ttacgataga aattaatctt
    44461 ttgcatgatc tgcaatctcc ttcagtcgcg ccacgataag tgccatttcc ccttccgcca
    44521 ccgtacgcgg atcaaggcta aaaacgccct ggtggaggtt ataccgccgt gcataaatcg
    44581 cgatatcgcc accacgcagt tgcgcttcga cgacgcgagc gtcaactccc aattcctgcg
    44641 catttactcg aatacgaata cgccagattg cacgcccggc ttcatcctgc tcaatatcag
    44701 ccgttaaccc gtgaatcgca gaaatcgctt caaccactgg ctggagctgt tccgcggtga
    44761 tgactgcctg cccctggtga taattctcca gcgcgtaaac cagcccaacc atgttctcct
    44821 tgccgatttt catcgccctg gcaatgccgt gatgctgcgc tttgcaggct gcaatccaga
    44881 tttttttacc agtgataaac ccggaggtcg gcgcgttaaa ggcttttgcg ccgctgtaga
    44941 ccaccatatc cgctccgctc accacccagg cacgtaaatc ctcttcggcg gcggcatcga
    45001 caatcagcgg caggttgtac atctgcgcga catgtgcgaa gtcctcgatg ttcaacattc
    45061 ccttctgcac gcagtgatgc gacttcacat acagcagcgc ggcggtcgcg tcggtaatgg
    45121 cactctccag ctgccagaga gccgccaggt tacttgaacc cacctccaca acgcgcccgc
    45181 cacctaagcg gatcgcgctg aggatcgatg caccgtaatc gacgttatgg ccgcgcagca
    45241 tcaccacttc attggccatg ccggagctat ccggcatgag cgccacccgg gccttgtcgc
    45301 cgcgtgtaat ggcggcggcg acagcgatgg cgatccctgc tgaggcacag gaggtaacgt
    45361 agctatcctc cgccccggta aagcgagaga ccagctcgcc ggttttgtcc accagctggt
    45421 caatctccac gaaagaggat gccgcgcgcg cggtggcctg catcacctcc ggcgcgacgc
    45481 tggagacgcc gagaatcgtc atcttgccgc aggcgttaat cacctgtttc agtcccagtt
    45541 gttgatagat attctgcgtc atggcttaca gcactcccag cagcgagcag accacgctca
    45601 acgccacaat cgacagcaga atcgtggtgt aacgcggacc ctttttcacc aaatagaagt
    45661 agatggcgaa cactgccgcc agcggcagca ggccgggcgc gatggagtcc agaatctgct
    45721 gtaccaccac ttcagagcct ttcagtgcgc taatttttaa aggtgaggtt atcttgacaa
    45781 agctcgcgga tagcgcgccc atcatgataa gccccagtac gttagcgccg tagataagct
    45841 ccttgatgcg ccctccctgc agcagactga taatcgaatc gcgccccagc gtatagccct
    45901 tgtgcaccat gccgtagctg attgccagcg taatcgccgg gtagagaatc agcggcaaaa
    45961 taccgcccat cgcgctgcca ttggcggcga acgggataaa aatagcaatc aacagcggca
    46021 tcaccgccgc ccagatgatg gagtcgccca tcccggccag cggccccatc aggccggttt
    46081 taatcccggt gatagaggca tcgttaatcg cctcgccgcg ggtcttttgc tcttccatgg
    46141 cgatggagat cccctggatc accgcgccaa acgtctgctc tgagttgaag aagttcagat
    46201 ggcgttttaa cgcctccacc tgctcctcct tttgcggata gagctttttg ataatcggcg
    46261 tcatcgaggc gcagaaaatc aggctctgta aacgctcata ggagctggaa acttccgcgc
    46321 ccagccagta gataaaccac gctttggtga tatccgcttt ggtcagcgcc ccggactccc
    46381 gcgcgcgctc aaccagttca tgctgcatta cgtcgtcgct cattatgccg ctccttcatt
    46441 tttcgccagg cctttaatca ggaaggccac gcaggtgccg aaaatcgcca tcgccatgat
    46501 gtccaccttg aggtacagca ccgcgaagaa tccgccgata aaccacggca gcaggctctt
    46561 tttaccaatc accataatgg tgatggcgaa gcccagcgcc gggagaatcc cgcccatgat
    46621 ttcaaacgag tgggtcagcc agtgcggcat cagcttgagg aaactttcca ctacgttctg
    46681 gccgaagtag ttggcggcga acaccactgg gaaacgtagt accagcccca gcagcgccgg
    46741 gtagagaaag gcgcagcgca taatggctga catattggcc gtttccgcgt gtttatcggc
    46801 catatgcacc caggccgcat ttagcgtgcg acgcaactgg tcgaggaata cgccgataac
    46861 gccaaatgga atcgccagtg caatcgccag gttcgggtcc atattggcct taacggcgat
    46921 aggaatggcg atacaggccg ccagcgccgg gtctgacggc acattgccgc ctggcgttga
    46981 ggtcacaccc agatagacca actgcatccc cgcgccgata atcatcgccg caggcatatt
    47041 gccgagcagc aggccgacaa agaccgccac caccactggc tgtaacagca tggcggagaa
    47101 ggtatagcca aggcgcaacc gggcaaacca gtaatacagc cccatcaggc ttgcaaaaag
    47161 taaggtatcc ataatttgtt atcccatgtt cagagggtca gaattttttc aggatatcgt
    47221 ccagcgactg cggtttatct tccgggatgg tctggaaaac agccttaatg ccgcgacttt
    47281 tcaaatcgtt aagaatgcca acgtcttttt catccagcgt tatgttctgg aacaccgctt
    47341 tacggtttgg cccgccgcct aaaccgccaa cctgaatatc agccacgtca aagccctgct
    47401 gcaccgcttc ctgaatcgcc gccagcgacg ggaacagcac cagcacgttg ccgtcgccca
    47461 gctggttctc tttccacgcg gccgcgaagc tctggttgct aaagcagtcc actttgatat
    47521 tcggcggcgc ggccatcagg tagatatttt tcataaacgg atcggcgtcc agctcgtcgc
    47581 tgaccaccgc aatacggttg gcctgagact gccctaccca cttcgtcacc acctgtccgt
    47641 ggataagacg gctgtcaatg cgacataaca cgatgtttgc catacgattt tccttactgt
    47701 tgtaattgac gaatatggga gacgacgtcc aggcaactgt tgcgcccagc ttccaccaga
    47761 tcggccacgc aggcggtcag tgggccatgt tcgcgcttgt ccagcgcctc cagcagcagc
    47821 gaggcattaa gcccggagat caccgccacc ggataatcgg cgctcagccg cgccgccacg
    47881 ttagaggtgg tgccgccgat aaaatcggtc acgataagcg aaccttccgg catggttttc
    47941 accacggctt cgacgcgctg gtaaaactcg cccagcgtat cgacgggcat cagcgcgatt
    48001 tccgtcacgc cttcgatttc gcccatcacc atgcgcagac tgttgcagag ctgcagcccc
    48061 cagccgccgt gagttagcag cagaatttca ggtaacggat gaataggtac agttgtggtc
    48121 aaaatagcct cctttgctcc agtgtttaca tttcatcagg agcaaaggac gtgccagaat
    48181 ttgtgtcacg cacagaggaa atgagattgg atggggcgtt aagccccgta aaccagggga
    48241 tcagctgtag agcagttcgt agatataaaa atattctgcg tccgataaac ggatagcgta
    48301 agcctcttca atcggcaaaa aggaggattt gatgacgcta aaggcacgag cgtccagatc
    48361 aagccggttt tctagcgcca tctgtaacgg tttgcggtta atcacgatac gctcgaccat
    48421 gcagcagcag tggatcagaa aacgtagtgt cacctggcgg ctcggtttca gggagagcgt
    48481 gttggttaag tagttcagaa catcctcgat ctctttcaga attcgctgcg ggttgagcac
    48541 cgaaatatgg ttgatgatgc tctccatggt cagcgcgctg ataaagcgca tggcgctgcg
    48601 ctccatctcc agtcggcgct cgctgcttga gagatccggc gtcagcaggc tgagcaccag
    48661 ctccggccct tgctcggaaa acagttcctc cagcgagata aacggtatat ccggcagtcc
    48721 cggctggaag gtgccgacga tccctgccag ccgttcactg gcattaagcg cctgctgaat
    48781 acgctccagg ctgcgcacct cgttatagtc gagaatcacc atacgcgtgt cctgcgacat
    48841 cagctcgcca aagctctcct ccagcacctt tttgatcttt tccgccgtcc ccattccggt
    48901 tatacatgag ataaccagca ctttgccacc gctctcttgc tgcggcgtgc agagccgaca
    48961 agggatattt ttactctgca taagcacagc caattgcgat agatcgccag tttcgtagct
    49021 taaatccagc ccaacctcca gcaggctggt cagagtgata tttggcaaca acagaacgtc
    49081 aatctggaac agcttgctga tggtgctgcc aaaatggacc agcgatccga tatccaccat
    49141 cagtatcagc cgctgccagc accggtcctg aatcatccgg gtcagggttt ccagggtgtc
    49201 atgcaccgac tgctcaaatg gcatatcaat tgcgtagaac agatcgcgct ccagcacccg
    49261 gttcacatat tgcgcctggc tcgttgcggt ggtcgcaccg tgagcgatta gaatcacgcc
    49321 gcaatccggg ctggtgtcga tgcgctggcg gtagtggcga cactctttca ggaacaggca
    49381 cagccagacc acctccgtcg ccgggcactg aatatgcaac aactcattga ttttgcggca
    49441 cagaagcgtg gcattgtcat actcatcttt acagcgatcc agaatcaggc tggatgaata
    49501 gagttgaggt ataagccctc gccgcacata gccaatcagc gcaagaaagt gtttacgtaa
    49561 cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc tgctccacgt aaccgatcag
    49621 cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt ggatgcgccg cactatggcg
    49681 gctataaaga ccatattcaa aaatcgaact gagtttgttt ttcaggatag ccagcgtttc
    49741 tgcgggcggc acgttactgt tgcgcagatt gacatattca cgagtcagga aactgtaaaa
    49801 caggtcgctc tcttctattt ccgcgctggt cgccaacgaa ttttttaacg cgggcaacgt
    49861 acgcgcatcc acgttcagac gatctttacc gccaaacaac gcatcgacca gcagacgctg
    49921 ctctggcgtg gtattaaacg acatctccgc cagtcgttta tctaactcta gcgtatcatt
    49981 acgctcggtc attcccgacg cccacgcctg cgcgcacaag aactgaatat cacttttaag
    50041 ctggccgata ttgccttcca ggggtttact taacaaccaa tgcaagagcg ttttatcaag
    50101 gcttatggta cgttcaattt tacgactttc acgctgaaag aagcttacaa tcaattcgag
    50161 ctgctcctcg accgaacgct ggcgaatacc cggtaagtca atacatacct ggatacgccg
    50221 ctggaaggta cgcaatagtg ccgagttgac cgactctgta gtggcgcaaa taaggcgcac
    50281 cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg tattcacctt tatcgagaat
    50341 agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc acttcatcca gaagtaaata
    50401 acctccgtca gcttgctcca ccaatcccgt tttattctca cttgccccgg taaatgcccc
    50461 cttatggtga ccaaacagat gtgaggaaag caactccgga ttatgagcat attcggcaca
    50521 gttaaagtaa accaacggag gtggcgaacc tatggtgtgc ttgcaagcga agcaatgcat
    50581 taactcagca aagaatgttt tgccaacacc agatgcaccg gttaacagca catgtagtcc
    50641 gtggggataa agcaccgctg cgcggccttt ttctaccgca tcacggagac tacggtcgta
    50701 tccgataagt ccggaaaacg gatcatcatc tgtatatttt tctttctttg gcaataaact
    50761 agccactgaa tgaactatgc attcgcaatc atccagtttt cgattaatga gcaattctag
    50821 tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt tttaccacca ggccttcatt
    50881 ccataattga ttaagatctt tactaaccga gttgcgtgcc agcccaaggt taaaaccaat
    50941 cgcctcagca gtaaatgctt tctccttcgc taaatcagac agttccagcc catacgtcag
    51001 ccgctccagc tccttgtgaa taacatcaat ccgtctcatt tctttaccgc ttaaaaaaca
    51061 atagttgttc agaacataca ctatttgtaa acgtaagaat gagaactatc gggaagaatt
    51121 aatgttcctt gtggcaatct ggtttctgaa tattcgtgtt ttaaatatta cttatggtct
    51181 gaaacaacca ggcaggtgaa cagagctaag aaaaagtagc gtagaaatat aaaaacatat
    51241 atcgcactga tgaatgtttc ccggcatgac tatgcacacc gggaaatatt tagtaaatta
    51301 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
    51361 agcagtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
    51421 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca gaccgccaat
    51481 taccggcgta ccggttcccg gcgcaaatgc aggatccagg cagtcgatat caaaggtcag
    51541 gtaaaccggc atatcaccca caatttgttt cacctgggcg ataacgtcat ccacgctgcg
    51601 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa attcggtacg
    51661 aataccaatc tgcacggaat gattcggatc gatcagacct tctttcggcg cggtatagaa
    51721 catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg
    51781 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
    51841 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
    51901 ctgcagcttc tcgctcatct cacgggcatc gccgaaagca tataccagat caccgcagtc
    51961 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
    52021 cagattcgtc gaaacctgac ggatagctgc cggaccgtgg cgtccccccg cacgaccaga
    52081 agtggccata tcgaacggca caccagtaat cacccagtca gcatcgctgt catacggctg
    52141 gaagttcatc ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg
    52201 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
    52261 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
    52321 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
    52381 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
    52441 gccttgtaat tcaaaggcgg caaagccgat taccccggta ttacgcacat cgccttgcgc
    52501 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cggtatgttt
    52561 atctttgcgg aatcctttaa tcgctaacga atctccggca ttcacaatat agccgttgtt
    52621 attcagcgaa ccttgcttgc cgttcagcac atccatgcca tcaaccgtag caacgatctc
    52681 gtagttggtg ttacgactgt aattacggac atataattga taattcattc cgactctggc
    52741 atggacaata tagcgtggtt cattattgga tgaatcataa acacgcgtaa taggtagaga
    52801 attaaaatta ccatctcgaa tagcatattc cagttggtta atacgaatgc tataaacgcg
    52861 gtcatagccg gaaggaacat tagtcgaata actaataacg atcatctctg caggatcgcg
    52921 ccaggctcgt tcagcagata cgccctggac cgatgagtgc acatccccac cccaggcggt
    52981 gccgacattg cccgatgcac tgctattata ctgcgcatta ctggaacaac cggccagcag
    53041 cacgactaat gccaccaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc
    53101 atttaattaa tgcagcgatt gtagggagat tatttaataa gaaaagcagc cagattattt
    53161 ttaagaatat ataaaaagaa agcgggccga tttacgtaaa taaatcgacc cgccagcgga
    53221 ataatcttaa ttattcatct tcaagataag tataaccgta caaacctgcc tcgaactctt
    53281 caaggaactg ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact
    53341 gggttaacag cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc
    53401 cttcgtcaga cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt
    53461 cggtatcacc gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa
    53521 agaaaccgag catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa
    53581 taccatcacc atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca
    53641 gcacagcgcg gcgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact
    53701 ggtcgatccc ccacgcatcc ggcatcgact ggaacagcga gaagttgacg tacattttgt
    53761 ctgccatacg ttcctgcagc tcgtcgataa tcggacgatg agcacggttt tgcggatcca
    53821 gttgcttctg cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt
    53881 gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt
    53941 cgtgtaacca ttcacgcaaa gaacggcgag ttcccggttc gtgcatctcc tgccaggttt
    54001 cccacatgct ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt
    54061 cgttacgttc cacgccgatg atattcgata ccagcacggt gtgatgcgca gttaccgcac
    54121 gacccgattc ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcaccaa
    54181 tcgcccagat gatgttgttg gcgtattcat tgaggccgta gtttacggag cagtcagact
    54241 gcgaacgggt gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat
    54301 tgacgcccag cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cctgtcgcga
    54361 tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga
    54421 gacgcccggc ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga
    54481 acttcgattt ttccccgccg gaggactgcc atttacccga gccctgcgag gccagacgtg
    54541 cacgcacgcc cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga
    54601 tttctgacat cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg
    54661 ccaggcggat atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc
    54721 cagcatgtgc cagcaccgcc atcaactctg ctttggaacc ggcttccaga cccagcggtt
    54781 cgcccgaatg aatcagggac tcgatcacgc ggcggtgctg gttaactttg atcggataaa
    54841 caaggaagta atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa
    54901 tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac
    54961 cctgtgcttc acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg
    55021 ggtccgggca cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc
    55081 aggcaatatt gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct
    55141 gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt
    55201 cagacatagc gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc
    55261 ctggaactgc gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc
    55321 caactgcgca accggtttct ttttcataac attattaagc acataaccga acgtaagtgt
    55381 gacagttcgg cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc
    55441 tcagtaactg taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca
    55501 ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag
    55561 gttaagcagc agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg
    55621 aacccacaag ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt
    55681 cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc
    55741 aaaagataaa tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac
    55801 agaatgagac acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc
    55861 atccagatgt taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc
    55921 agagtcgtgg taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat
    55981 tctctttagg tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa
    56041 gggcatcctg acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa
    56101 caggatccga aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt
    56161 ggcggcgaaa tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt
    56221 cgcgaaattg gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg
    56281 agcgctatcg gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg
    56341 gaacagggcg cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg
    56401 ctgatgccag cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt
    56461 aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat
    56521 gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ttctgaagag
    56581 atcgaccaga aatcgctgca agaagcggta atggaagaga tcatcaagcc aattctgccc
    56641 gctgaatggc tgacttctgc caccaaattc ttcatcaacc cgaccggtcg tttcgttatc
    56701 ggtggcccaa tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc
    56761 ggcatggcgc gtcacggtgg cggtgcattc tctggtaaag atccatcaaa agtggaccgt
    56821 tccgcagcct acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat
    56881 cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg aaccgacttc catcatggta
    56941 gaaactttcg gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc
    57001 ttcgacctgc gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa
    57061 gaaaccgcag catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa
    57121 gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg
    57181 ccagcctcgc gctggccttt ttcttttacc tcaccgcggt ggataggcgt tcacgccgca
    57241 tccggcaaaa aaaccgcctg cacaataaca tcattcttcc tgatcacgtt tcaccgtaaa
    57301 ttatcatcac aactgaaacc gattacacca accacaacag acaaagattt gtaatatttt
    57361 catattatta ttcggttttc acagttgtta catttctttt cagtaaagtc ttaattgcag
    57421 ataacagcgt ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac
    57481 taagataaat gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt
    57541 cgtactcacc tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga
    57601 cgctaaaaaa caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc
    57661 tctggcggga ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat
    57721 tgcagatgaa ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt
    57781 cggtgcggca gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa
    57841 gagcctgatg atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc
    57901 aaacgttgaa gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc
    57961 ttataccgca ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat
    58021 ctcgatgtat cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgatacagc
    58081 cttcagctac accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt
    58141 gctgctgatt ggtgtcttct tcctgccaga cagcccacgt tggtttgccg ccaagcgccg
    58201 ttttgttgat gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg
    58261 cgaactggat gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa
    58321 agagaacagc aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca
    58381 attcaccggg atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta
    58441 taccaacact accgagcaaa tgtgggggac cgtgattgtc ggcctgacca acgtacttgc
    58501 cacctttatc gcaatcggcc ttgttgaccg ctggggacgt aaaccaacgc taacgctggg
    58561 cttcctggtg atggctgctg gcatgggcgt actcggtaca atgatgcata tcggtattca
    58621 ctctccgtca gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc
    58681 catgagtgcc ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg
    58741 cgattttggc atcacctgct ccaccgccac caactggatt gccaacatga tcgttggcgc
    58801 aacgttcctg accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc
    58861 tctgaacgta ctgtttatcc tgctgacatt gtggctggta ccggaaacca aacacgtttc
    58921 gctggaacat attgaacgta atctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca
    58981 cgattaatct cctcaggctt cctcccatcg cggaggaagc cacctcttgc agtcatcttt
    59041 tcttcgctct atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccagcagg
    59101 ccgttatgcg ccgcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact
    59161 acccggagcc aaaactctct tacacccagc gcggaacctc cgccggaaca gcctggctgg
    59221 aaagctatga aatccgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg
    59281 aagaagtggt accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag
    59341 cgccacatgg caaagagtgg aagtggatga tggaaagcgt gctgggcgtt cccgcccgtc
    59401 gtacgcatca gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt
    59461 gccaggagca tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct
    59521 atcgctgtgt tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa
    59581 ctttcctgat ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt
    59641 aacacggagt aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca
    59701 ttttccggcc cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg
    59761 gtaaaagtcc acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag
    59821 ggcaaaaaag gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac
    59881 cgagccagcc gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc
    59941 cagtgctggc aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg
    60001 gaaagcgata tgcataacct gcaaccgtca gtcggcgagg tgaatggcga tcgcggcaac
    60061 tttatgtaca gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc
    60121 gatttcaaag aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcacgcacc
    60181 tacttctata tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc
    60241 aacgcatgga acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc
    60301 gcgaaggtgc aaggcaatca taatccgtat gtgcaacgcg cttgccaggc gcgaaagagc
    60361 taacctacac tagcgggact ctttttgtta acccctaccc cacgcgtaca accgcgtggg
    60421 gagacgacgc ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac
    60481 cagccattct cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg
    60541 catggggccg gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga
    60601 aattaccagc gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga
    60661 tcgcgaatct ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga
    60721 atttacgatc cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga
    60781 gcgctgcggc gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa
    60841 gattgcaatt gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgcccagc
    60901 gatggatctg gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc
    60961 gcgcgccagt aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat
    61021 tggcccggaa ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac
    61081 tgatatcctg ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac
    61141 cgcgctacaa gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg
    61201 catcgtgatg gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt
    61261 gctggaagca cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct
    61321 gatcaatggc gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga
    61381 gtggttttcg ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat
    61441 gcgtaaagac ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc
    61501 cgaagagaaa gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa
    61561 actgtttacc gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc
    61621 gcagctaaaa gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat
    61681 gggcggcgcg tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga
    61741 aaccctgact gagcatggca ctcgctactg catggcgcaa aattacctgc cagccattaa
    61801 agatggcgac aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg
    61861 tattccgcag gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg
    61921 tccgctgacg gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgt tgaaagaaaa
    61981 agggctgatt tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac
    62041 cagcccaacc tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt
    62101 aatggatgcc atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc
    62161 gttgagactc tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt
    62221 gaaccaggaa acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc
    62281 tgctctccag gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa
    62341 tggtgcaatg gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct
    62401 ggaaaagctg aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt
    62461 tatgctcggc ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa
    62521 ttttgcttcc agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct
    62581 ggaaacgctc ggcaccgata aacaaccgtc tgacgtattg gtggctctgg gttatgcctc
    62641 ctgggaaaaa ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc
    62701 ggatctgaat attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact
    62761 gattggtgtg gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc
    62821 ttactcgcct tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc
    62881 ggcactgctc gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc
    62941 atcgaacgtt tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat
    63001 atggacggca ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat
    63061 ggtcgtttcg gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt
    63121 tccggtctgt ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc
    63181 tctgcggtta ttattctcga aagctatttc gagcagggat attaaggcga tctaaaagcc
    63241 tggcggatag tcgttaccgc catcttgccg gatgcggcgt gaacgcctta tccagcacac
    63301 atctggcagc ggctgtaggt ctgataagac gcgttagcgt cgcatcagac atttattgcc
    63361 tttataggcc ctataaactt gcgcatcggg catggcaacg tcacaaacgc ccttccccca
    63421 cccgctgctg ataactctgc tgaaacgtta acatccccac ctgctgcccg gtttgaataa
    63481 catgcggtaa ctggtgggtt ttcccttcgc gaatcaaatt ccccaccgcg ggagtgttaa
    63541 tcaataattc aaacagcgcc acgcgtcctt cctgtttatc cacttccagc ttttgtgaca
    63601 acacggcccg taaactacct gccagttgat tacgtacggg atctttttcc tgcgccggaa
    63661 atgaatccac cagtcgctca actgcctgcg cggcaccacg cgtatgtaat gttgccagca
    63721 ccaaatgccc ggtttctgcc gccgtcagtg ccagacggat tgtctcgctg tcacgcagct
    63781 ctccgagcaa aatcacatca ggatcttccc gcaatgcggc ccgcaatccc gatgcgaacg
    63841 tcatacagtg caaaccaatt tcccgctgct ggatcaaaca tcgctggctg gcatagatat
    63901 attcaacagg atcttccagc gtcagaatat gcgcatcggc atgttgattg agatagccaa
    63961 ccatcgccgc cagcgtggta gatttgccgc tccccgtcgc ccccgtcacc agaatcaggc
    64021 cattctcgct cttgagtaat tccggcaata ccgttggtgc gccaagctgt tcgagctgtg
    64081 gacagtgcga aggtaacaac cgtaatgcca gagaaatacc ttgccgttgc gcgaacgcac
    64141 tgccacgcaa ccgctggttt tccgccagcg acacggcaaa atccagctgg ccattttcca
    64201 gcaatattgc ccgctgatcg tcatccagcc actcccgcag tagctcttcg acgtccggcg
    64261 catcaaacgg cgcagcttcc attagcccgc gaatgcgcca tcgtgcggac caggcgctgc
    64321 acaggtgtag atccgagacg ttatgcttta cactaagggc cacaatttct tccatattca
    64381 tactaagatc ctcggaaaat gaacgatatt gcgcataacc tggcacaggt ccgggacaaa
    64441 atctcagcgg ctgcaacgcg ttgcggccgt tctccagaag aaattacgct gcttgcagtc
    64501 agtaaaacaa aacctgcgag cgccatcgca gaagccattg atgccgggca gcgtcaattt
    64561 ggtgaaaact acgttcagga aggggtagat aaaattcgcc actttcagga actgggcgta
    64621 acaggattag aatggcattt tattggcccg ttgcagtcta ataaaagccg ccgggtggca
    64681 gagcatttcg actggtgtca taccatcgac cgtttgcgca tcgctacccg cctcaacgat
    64741 cagcgcccgg cagaacttcc ccctcttaac gtactgattc aaattaatat tagtgatgaa
    64801 aacagtaagt ccgggattca actggcagaa ctggacgagc tggcagctgc ggtcgctgaa
    64861 ctaccgcgtt tacgtctgcg cgggctgatg gcaatccctg cacctgagtc agaatatgta
    64921 aggcagtttg aagttgcacg ccaaatggct gtagcatttg ccggactgaa aacgcgctac
    64981 ccgcatatcg acacgctctc tctgggaatg tcggacgata tggaagccgc cattgcggca
    65041 ggtagcacga tggttcgtat cggcactgca atttttggtg cgcgtgatta ctctaaaaaa
    65101 taaggaatta aaggaacgcc atgaatacgt tgactttcct gctttcaacg gtcattgagc
    65161 tgtataccat ggtgctgtta ttacgcatct ggatgcagtg ggctcattgt gatttttaca
    65221 accccttctc acagtttgta gtgaaggtaa cgcagccaat tatcgggcca ctgcgccgcg
    65281 ttattccggc aatgggcccc attgacagcg cctcgctgct ggttgcctat attctcagtt
    65341 ttatcaaagc catcgtgctg tttaaagtgg tgaccttcct gccaatcatc tggattgccg
    65401 gtttactgat tctgctgaaa accatcggcc tgctgatttt ctgggtcctg ctggtgatgg
    65461 cgattatgag ctgggtaagc cagggtcgta gcccgattga atacgtgctg attcagctgg
    65521 ccgatccgct gctgcgcccg attcgccgcc tgctaccggc aatgggtggg attgatttct
    65581 cgccgatgat cctcgttctg ctgctgtatg tcatcaatat gggtgtcgca gaagtattac
    65641 aggcaaccgg aaacatgctg ctgccggggc tgtggatggc gttatgagtg ccgtaacagt
    65701 taatgatgac ggtctggttt tacggctcta tattcagccg aaagccagcc gtgattctat
    65761 tgtcggttta catggcgacg aagttaaagt cgccattacc gcgccgccgg ttgacggtca
    65821 ggccaacagt catctggtga agtttctcgg taagcaattc cgggttgcca aaagccaggt
    65881 ggtgattgaa aaaggcgaac ttggccgcca caaacaaatt aaaatcatta atccgcaaca
    65941 aatcccgcca gaaatcgcgg cgttaattaa ttaggtatcc tatgcaaaaa gttgtcctcg
    66001 caaccggcaa tgccggtaaa gtgcgtgagc tggcgtcgct gcttagcgac ttcggtcttg
    66061 atatcgtggc ccaaacagac ctcggcgtag attccgctga agaaaccggc ctgaccttta
    66121 tcgaaaacgc gattctgaaa gcgcgccatg cggcaaaagt gaccggttta ccggcaattg
    66181 ccgacgactc tggtctggcg gtagatgtgc ttggcggcgc gccgggcatt tactccgcgc
    66241 gttattccgg tgaagacgcg accgatctaa agaatctgca aaaactgctg gaaacactga
    66301 aagacgtacc ggatgaccaa cgtcaggcgc gtttccactg cgtgctggta tatctgcgtc
    66361 acgcggaaga tccgactccg ctggtatgcc acggtagctg gccgggcgtg attactcgtg
    66421 aaccagcggg cactggtggc tttggttatg atccaatctt cttcgtacct tccgaaggga
    66481 aaaccgccgc cgaactgacc cgcgaagaaa agagcgccat ttcccaccgt ggtcaggcat
    66541 tgaaactgct gctggacgct ttacgtaatg gttaaattac cgccgctgag tctctacatt
    66601 cacatcccgt ggtgcgtgca gaaatgcccg tactgcgatt tcaactctca cgcgttgaaa
    66661 ggagaagtgc cgcacgacga ttacgttcag catctgcttt gcgatctgga caacgatgtg
    66721 gcttacgctc agggccgtga agtaaagaca atctttattg gcggtggtac gccgagcctg
    66781 ctttccggcc cggcgatgca aacgctgctg gacggcgtgc gtgcgcgttt gccgctggca
    66841 gcggatgcag aaattactat ggaagcgaac cctggtacgg tagaagccga tcgctttgtc
    66901 gattatcagc gtgctggtgt gaaccgcatc tctattggtg tgcagagttt tagcgaagac
    66961 aagctgaaac gacttgggcg tattcatggc ccgcaagaag cgaaacgcgc ggcaaagctg
    67021 gcgagcgggc tggggctgcg tagctttaac ctcgatttga tgcatggact accggatcaa
    67081 tcactggaag aggcgcttgg cgatctacgc caggccattg aactgaatcc gccgcatctt
    67141 tcctggtatc aactgaccat cgaacctaat acgctgtttg gttcacgccc gccggtactg
    67201 ccggacgacg acgcgctgtg ggatatattc gaacaggggc atcagttatt aaccgcagcg
    67261 ggttatcagc aatatgaaac ttccgcttac gccaaaccca gttatcagtg ccagcacaat
    67321 ctcaactact ggcgctttgg cgactacatc ggtattggct gcggcgcgca cggcaaagtg
    67381 accttcccgg atgggcgcat tctgcgtacc actaaaacgc gtcatccgcg tggttttatg
    67441 caaggaaggt atctggaaag ccagcgtgat gtcgaagccg cagataagcc gtttgagttc
    67501 tttatgaatc gcttccgtct gctggaggcc gcgccgcgcg tggagtttat tgcgtatacc
    67561 gggctttgcg aagatgtgat tcgcccacag ttagacgagg cgattgccca gggttatctc
    67621 accgaatgtg cggattactg gcagataacg gaacatggga agctgttttt aaattcgctg
    67681 ctggagcttt ttctggctga gtaaacttgt attgccggat gcggcgtgaa cgccttatcc
    67741 agccgacatg tggcagcggt tgtaggtctg ataagacgcg caagcgtcgc atcagacgtt
    67801 gattgccgga tgcggggtca acgccgcatc ctgctacaaa tcgtgcacta tatcaaactt
    67861 acttaatcac cccattgcgc cgcagttgtt cggtatcttt tttccagcgt tcattagctt
    67921 ggttgatatc gtcaattttc atcaaaatcg cgactttttt actgtttatt tctgaaactt
    67981 tcatcgttaa cggatattgc cctttttcaa gcgttaccca gtcatcctgc aatttacggc
    68041 tgagtttttg tgattgcaga aaagtctgtc gccgttgttc gttgatatcg aaatctcgtt
    68101 tctgctgaat cggcacttca taacactctt cactctgttt atgacatgcc gcaatcgccg
    68161 gttgataaac ctctttgtca tatttaaccg cgaacgcctc gctatcgttc tgtttattaa
    68221 actcatcacg ctgttggtga atttttttaa aggcttcttc gcgggtcatt tgcttaccgt
    68281 ttgcatcttt tccccaatat gcatccgctg ctttctgcgc cgccaccatt tgcttatcaa
    68341 gcgctttcac ttccttttcc agcgcaggaa tcgagttctt ctgggtattc aacgcagatt
    68401 caagctgact aaagcgataa ttgaaatctt cgctattcac gatcagatat ttattattcg
    68461 ttttgatatc gtcgaccacc cgcccacgat cgctggctgc cgcctgaaaa gagtaaaagt
    68521 tcaccgacca gccagacgcg ggcgttcctt tactggttaa catcgccgag aattttaccg
    68581 gtttatcttt cgtccaggtc tgttcgagca gttcgtaatc tgccaactga ccgacccagg
    68641 tatagagatc gtcactggaa gagacatcgc cttccgctga ccaggtggcc tgattaccct
    68701 tagcatcaag attttttaag gtgatagcat caagcttgag aatgccgtga tactgctttt
    68761 tgaattgatc ttttaagata ttttcagttg gcggttcgcc atctgcccaa gcattaccag
    68821 tcatcaacat aagcagtgcc gtgccaacaa tccattgctt tttcacccga aaaacctcac
    68881 gcggaataat ttattccggt gattatcatt agggtaatga aaattggcaa tggacgtaag
    68941 tctgaagtga aaaagccccg gcacgatacc ggggcgaggt gattagtact gattgaagat
    69001 ctgctggatc tgctgcggat ctttggtttg cgtcagagcc agctgcagca ggacgcgcgc
    69061 tttttgcggg ttcagcgtgc cagaggcgac gaagccgtat ttcgcatcat ccacttcagc
    69121 atcctgagtg gtagcacccg tcggtacgcg ggaagaacgc actactgcag tgccgttttt
    69181 cgcggcggtt gccagggtgt cgaacacgga tttatacagg ttaccattac ccacaccagc
    69241 gctaacgatg ccatcatagc ccgcatctac cagtgcttta gccggaagat cggatgcgtt
    69301 agcgtagtta taaacgatgc cgactttcgg cagctcattc agcttagaga catcgaatgg
    69361 cgtatcgctg gtgtgcttac gtgccggggt acgttggtag tcaatcttac cgttatgaat
    69421 gtatcccaga ggaccgtagt taacagactt gaaggtcgct acgtcggtgg tgttggtttt
    69481 ggtgacgtca cggccatcaa gcacggtgtc attcatcact accagcacgc cacggttggc
    69541 ggaggcttta tcagctgcgg tcactaccgc gttatacagg ttgaatggac cgtctgcgct
    69601 catagacgtg gacggacgca ttgcgccgac catcaccacc ggtttgtcgc atttcaccgt
    69661 caggtcgagg aagtaagcag tttcttccat cgtgtcggta ccgtgggtaa tgacgaagcc
    69721 gtcggtctta tcgcagtcgg tgttaatttt tttcgccagt gtcagccaga cattatcgtt
    69781 catgtcctgg gagccgatat tcactacctg ctcgccttta acgttcgcaa tgtccttcag
    69841 ttgcggcacc gcattaacca gattttctac gccaacttta cccgctgtgt agttagattt
    69901 ggttgcggag tcaccaccac cggcaatggt cccgccggtt gctaaaatgg tgatattggg
    69961 taatgccaat gctgcaccac taaaacccat aaccagtgca gcaagtgccg tctttttgaa
    70021 aaactccatt tcattcctcc agttacgtga acgctacgca ttatccctta tctttgtatg
    70081 ggaaatttga cgttaaacaa tttacaacgt gaatatattt tggagatcta caaagttaga
    70141 ggcaggtaac aaaacgaaga attaaacggc ataaaaaagt attatgccgt cttaaaatag
    70201 aggattattt taaattcccg accagggctt tgcggctatc ttccagagtc acaacacggc
    70261 tacaaacatc tttgccaaac tgctggaaat ctttttcctg ctttttccac tcggtttgga
    70321 ttgaggattg cagccccccc aggcttccca gcacattctg taatgggtta ccgccgcttt
    70381 tcaacaccgc tttcgcgccc atttcattaa tgctgtcctg taaaattcca cccattgcct
    70441 gattcactaa ttgctggcct tcggcgcgaa cctgatcaat ggctttatag tgaaacgtca
    70501 ggccatcgct gcgcgtttcg ataatgcggt tcatctgctc tttcagttgc gcatcaagtt
    70561 tggtcagacg gctgcgcatt ttgctgcttt cgcccatctc ctgaacgata attttatcca
    70621 gcgcaatacg ggctttttcg acgcggcttt tcgcgccttc atcaatccac ggcagcgtgc
    70681 tgcgtagttc agcctgataa tccttcgcct gctcgcgctg ggcggcattc agggaatatt
    70741 gcttaccgtt atacatcacg ttgccgtctg gcgtgatcac cagattgccg ttttcgccct
    70801 tcacctgcac ggtttgcggg ctgacaatca catcgtcacg cggcgtgacg ctgcactggt
    70861 agtcggcgtg agcggtcatt gccgtcactg aaagtgctgc cgccagcagc attttgcgca
    70921 tcatagtctt ccctcaagaa aaaatcaggc cagcatttgc tggccccaga ttgataacaa
    70981 agtacgcgtt ttccatgccg gatgcggcgt aaacacctta tcccgcctac tcgatcgtgc
    71041 atctttccag gcctgataag cgtagcgcat caggcagttt tgcatttgtc atcgcccgta
    71101 tgctttctta gtcccaccaa acgtcgaaaa gttcgctggt gcgtacctca tccagtttgc
    71161 gctcttccag ccacttacgc acaatcgcct gatgttcttc ggtgcatttg ccgatttcct
    71221 gcatgcagat cagaccttcc caggccagat aaccgctgcc gtcaaaggcc agtttgttcg
    71281 gttcgataac ctcgttaata aaatcatcaa cggttttatc aatctgttct tccgatgtac
    71341 cttccgggaa tcgccatgcc accgaaaatc ctaattcctg gaattcgtcg atgtgcattt
    71401 ttttacgcag acgacggcta cggttctttg ccattatttc accctctcga acattaagtc
    71461 ccatactccg tgaccaagac gatgaccacg ttgttcaaat ttcgtcaccg gacgtgatgc
    71521 cggacgcggt acgtaatcat tgctctctga caggttttta taaccgtcaa tagaagacat
    71581 cacttcaagc atatgttccg cataaggttc ccagtcggtc gccatatgga atacgccccc
    71641 cagctgcagt ttgcttttta ccagttcggc aaacggcacc tgtacgatac ggcgtttatt
    71701 atggcgcgct ttgtgccacg ggtcagggaa aaagagctgc accatgcgca atgaattgtc
    71761 aggaatcatt ttatgcagca cttcaaccgc atcgtgacac atcacgcgca ggttgcttaa
    71821 accttcttca tgcgcagaag ccaggcacgc accaacgccc ggtgaatgca cttcaatgcc
    71881 gaggaagtcc tgctcagggc gatctttagc cattgccacc agcgacgccc ccatgccaaa
    71941 accaatctca agcgtcaccg gcgcttcacg gccaaaaagc gcggggaaat ccagcatatc
    72001 ttcgctgaac tcaacgccca tcaccggcca gtagttttcc agcgcatgtt cctggccttt
    72061 ggtcagtcgc ccctggcggc gcacaaaact acggatacgg cgcagtgggc ggccgttttc
    72121 atcaaattcc ggtgaaatga cgtcgttttt cataaagatt tagtcgcttg tgaaagtgtt
    72181 ctgaaaacgg gcattatcca aagttagttg ccggatgcaa gcatgataag gccgtggctg
    72241 cggaaagttc cggtttacac cctgccgtcg ctgtgctgca atcttgcccc caacaacagt
    72301 gaattcggtg accatgcaag cgtcgcaatt ttcagcccag gttctggact ggtacgataa
    72361 atacgggcgg aaaacgctgc cctggcaaat tgacaagacg ccctacaaag tatggctctc
    72421 agaagtgatg ttgcaacaaa ctcaggttgc gaccgttatc ccctattttg aacgctttat
    72481 ggcgcgcttc ccgacggtga ccgatctcgc caatgcgccg ctcgacgaag ttctccactt
    72541 gtggaccggg cttggctatt acgcccgcgc gcgcaacctg cataaagcgg cacaacaagt
    72601 ggcgacctta cacggcggta aattcccgga aacctttgaa gaagtcgcgg cgttaccggg
    72661 cgtcgggcgt tccaccgcag gcgcgattct ctcgctttct ctgggtaagc actttccgat
    72721 tctcgacggt aacgtcaaac gggtgctggc gcgctgctat gctgtaagcg gctggccagg
    72781 gaaaaaagag gtcgagaata aattatggag tttgagcgag caggtgacgc ccgcggttgg
    72841 cgtggaacgg tttaatcagg cgatgatgga tttgggtgcg atgatttgta cgcgctcgaa
    72901 accgaaatgt tcgctctgtc cgctacaaaa cggatgtatt gccgccgcca acaatagctg
    72961 ggcgctttat ccgggcaaaa aaccgaaaca gacgctgccg gagcgcaccg gctacttttt
    73021 gctattacag cacgaagatg aagtattgct ggcgcagcgt ccgccgagcg gattgtgggg
    73081 cggtttatac tgtttcccgc agtttgccga cgaagaaagt ttgcggcatt ggctggcgca
    73141 acggcagatt gctgccgata acctgacgca gctgaccgcg tttcgccata ccttcagcca
    73201 tttccactta gatattgtgc ctatgtggct tcccgtgtcg tcattcaccg gctgcatgga
    73261 tgaaggcaat gcgctctggt ataacttagc gcaaccgccg tcagttggcc tggcggctcc
    73321 cgtggagcgt ttgttacagc agttacgcac tggcgcgccg gtttagcgcg tgagtcgata
    73381 aagaggatga tttatgagca gaacgatttt ttgtactttc ctgcaacatg aagcagaagg
    73441 tcaggatttt cagctgtacc ccggcgagct gggaaaacgc atctataacg agatctccaa
    73501 agaagcctgg gcgcagtggc agcacaagca aaccatgctg attaatgaaa agaaactcaa
    73561 catgatgaat gccgagcacc gcaagctgct tgagcaggag atggtcaact tcctgttcga
    73621 gggtaaagag gtgcatatcg agggctatac gccggaagat aaaaaataaa aacagtgccg
    73681 gagcacgcct ccggcaactt gcataaaaac aaacacaaca cgcacctgga atgatgaaaa
    73741 aatatctcgc gctggctttg attgcgccgt tgctcatctc ctgttcgacg accaaaaaag
    73801 gcgataccta taacgaagcc tgggtcaaag acaccaacgg ttttgatatt ctgatggggc
    73861 aatttgccca caatattgag aacatctggg gcttcaaaga ggtggtgatc gctggtccta
    73921 aggactacgt gaaatacacc gatcaatatc agacccgcag ccacatcaac ttcgatgacg
    73981 gtacgattac tatcgaaacc atcgccggga cagaacctgc cgcgcatttg cgccgggcaa
    74041 ttatcaaaac gttattgatg ggtgacgatc cgagttcggt cgatctctat tccgacgttg
    74101 atgatattac gatttcgaaa gaacctttcc tttacggtca ggtggtggac aacaccgggc
    74161 agccgattcg ctgggaaggt cgcgcaagca acttcgcgga ttatctgctg aaaaaccgtc
    74221 tgcaaagccg cagtaacggg ctgcgtatca tctacagcgt caccattaac atggtaccga
    74281 accaccttga taaacgtgcg cacaaatatc tcggcatggt ccgccaggcg tcacggaaat
    74341 atggcgttga tgagtcgctg attctggcaa ttatgcagac cgaatcttcc tttaacccgt
    74401 atgcggtcag ccgttccgat gcgctgggat taatgcaggt ggtacaacat actgccggga
    74461 aagatgtgtt ccgctcgcag gggaaatccg gcacgccgag ccgcagtttc ttgtttgatc
    74521 ctgccagcaa tattgatacc ggcaccgcgt atctggcgat gctgaacaat gtttatctcg
    74581 gcggaattga taacccaaca tcgcggcgtt atgccgtcat caccgcctat aacggcggcg
    74641 caggcagcgt gctgcgagtc ttttcgaatg ataagattca ggctgccaat attattaaca
    74701 ccatgacgcc gggcgatgtt tatcagacgc tgacgacccg ccatccctct gcggaatctc
    74761 gccgttatct ttataaagtg aataccgcgc aaaaatccta ccgccgccga taattccatt
    74821 aaccgcccct gacgatgctc aggggcaaaa atgttatcca catcacaatt tcgttttgca
    74881 aattgggaat gtttgcaatt atttgccaca ggtaacaaaa aaccagtccg cgaagttgat
    74941 agaatcccat catctcgcac ggtcaaatgt gctttttcaa acactcatcc gcatcacgat
    75001 gtgaggaaat taacatgaat cttaagctgc agctgaaaat cctctctttt ctgcagttct
    75061 gtctgtgggg aagttggctg acgaccctcg gctcctatat gtttgttacc ctgaagtttg
    75121 acggtgcttc tattggcgca gtttatagct cactgggtat cgccgcggtc tttatgcctg
    75181 cgctgctggg gattgtggcc gacaaatggt taagtgcgaa atgggtatat gccatttgcc
    75241 acaccattgg cgctatcacg ctgttcatgg cggcacaggt cacgacaccg gaggcgatgt
    75301 tccgtgtgat attgattaac tcgtttgctt atatgccaac gcttgggtta atcaacacca
    75361 tctcttacta tcgcctgcaa aatgccggga tggatatcgt tactgacttc ccgccaatcc
    75421 gtatctgggg caccatcggc tttatcatgg caatgtgggt ggtgagcctg tctggcttcg
    75481 aattaagcca catgcagctg tatattggcg cagcactttc cgccattctg gttctgttta
    75541 ccctgactct gccgcatatt ccggttgcta aacagcaagc gaatcagagc tggacaaccc
    75601 tgctgggcct cgatgcattc gcgctgttta aaaacaagcg tatggcaatc ttctttatct
    75661 tctcaatgct gctgggcgcg gaactgcaga ttaccaacat gttcggtaat accttcctgc
    75721 acagcttcga caaagatccg atgtttgcca gcagctttat tgtgcagcat gcgtcaatca
    75781 tcatgtcgat ttcgcagatc tctgaaaccc tgttcattct gaccatcccg ttcttcttaa
    75841 gccgctacgg tattaagaac gtaatgatga tcagtattgt ggcgtggatc ctgcgttttg
    75901 cgctgtttgc ttacggcgac ccgactccgt tcggtactgt actgctggta ctgtcgatga
    75961 tcgtttacgg ttgcgcattc gacttcttca acatctctgg ttcggtgttt gtcgaaaaag
    76021 aagttagccc ggcaattcgc gccagtgcac aagggatgtt cctgatgatg actaacggct
    76081 tcggctgtat cctcggcggc atcgtgagcg gtaaagttgt tgagatgtac acccaaaacg
    76141 gcattaccga ctggcagacc gtatggttga ttttcgctgg ttactccgtg gttctggcct
    76201 tcgcgttcat ggcgatgttc aaatataaac acgttcgtgt cccgacaggc acacagacgg
    76261 ttagccacta attacgcaaa gaaaaacggg tcgccagaag gtgacccgtt ttttttattc
    76321 ttacttcaac acataaccgt acaaccgttt catgccatcc gcatcggttt cgctataaac
    76381 accttgcagc tccggcgaaa atcccggcaa caaattcacc ccttcttcca gtgcaaggaa
    76441 ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca cgccaggtgg
    76501 ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc gcaccaactc
    76561 cacttcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca ctgacgggaa
    76621 actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga catacagatc
    76681 gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg gatatttgtt
    76741 ataaacgctc ggcaacacct cagccagcgg cgagtcatcc tcaatatgct gttcaaactg
    76801 cgccaacatc tctaccagtt gtgccagctt ctcgtggctt tccgccggag ttaataagaa
    76861 cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca gatagtgcgc
    76921 cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat tgatacctgg
    76981 tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat atccttcaaa
    77041 gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg ctaacacaga
    77101 tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg ggcggaacag
    77161 cttacagcgc gcaagaatag ccttgcgcga ctcaataccc aactcaacgc actcagccca
    77221 cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg cagcaaacag
    77281 cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc gcttatgcgg
    77341 gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg acgtctgtga
    77401 gaatccagcc tgctgtttgt gcaccgactg ggtcacaaaa atccccggat cgttttcgtt
    77461 aagttctaac agcagcggcg agctatccgc catcatcggg ataaattgtt cgtaaccgac
    77521 ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca acggtatcga tcacctgacg
    77581 ggcgttatag acagttccgt cataggttcc cagctgaata atcgccaggc gaaacgggcg
    77641 cggcagctcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat actcttcatt
    77701 aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttgcgtgaag cttccagata
    77761 gaccggcgtc gcccccgcct gaatcagcgc gccgtgatga ttcgacttat ggttgttacg
    77821 gtcgaagagc accagatcgc cacgcgttaa cagcgcattc gtcaccactt tattcgctgc
    77881 cgatgtgccg ttcagcacaa aataggtttt atcggcatga aagactttgg ctgcgaattt
    77941 ctgcgcatct ttcgccgatc cttcatgaat aagcagatcg cccaatttta cgtcagcgtt
    78001 acacatatcg gcgcgaaaga cgttctcacc aaagaaatcg taaaaatggc gtccggcagg
    78061 atgcttttta aaaaacgcac catgttgatg tccagggcaa gcaaaggtgc tgttgcccat
    78121 ctcaacgtac tgcgtcagcg tgtcataaaa cggtggcagc aaattctctt catactgaca
    78181 ggctgcggat tccagctcca gccactgctg ctcgttgccg ttgattaccg ccgtaacgcc
    78241 cgcaggtaat tcaacagcat gttcggaata caaaaacacc ggtagatgaa aaccggtgcg
    78301 cttaagcaac gcaagaatgc cactgcgact atccgcagcg gtaatgacga ctgccgcgac
    78361 gtccgtaaaa tcagtatctc ccaacgccac cacgcgacga tgagaagaaa gtcgggatac
    78421 cagttcacta ctggcggcaa tattcattga tttcataagc gcaaacccgt ttcggggaag
    78481 taagaatacc ggacaaggtg gaaaaccctg ccccatgaga tatgggtcaa actggtcacc
    78541 agctccgacc gccagacatc agtaaaagca gaaacgctct gattttactg ttgtcctgca
    78601 gtgagcgtgc gttaacttca ccgcatgagc agtaacatag aaagggaaac gtttcgcgcg
    78661 aaacggcgat aagcgagaga atgtaaggag atggcgtgca tcgggcaaac tccgtacaga
    78721 gaggagaaaa ttcgcgcaat catggcacct ttcgctaaag cgtgcaagcc aggactttgc
    78781 gaccagacaa gccatcaaca aggcaaaaag ttcataataa gaaaatcaaa caatacagct
    78841 aacaggaact cttgtggtca tcggcccttt tatcaacgct agtgcagtct tactgggtgg
    78901 cgttctcggc gcactgctca gccaacgctt accggaacgt atccgcgtct ccatgacatc
    78961 tatttttggt ctggcatcgc tggggattgg tattttactg gttgtgaaat gcgccaacct
    79021 tccagcgatg gttttagcta cgctacttgg cgctctaatt ggcgaaattt gtttgctgga
    79081 gaaaggtgtc aatacagcag tcgccaaagc acaaaatctg tttcgtcact cacgtaagaa
    79141 gccagcgcat gaatctttta ttcagaatta tgtcgcgatt attgtcctgt tttgcgccag
    79201 cggcaccggg atcttcgggg cgatgaacga agggatgacc ggcgatccga gtattttaat
    79261 cgccaagtca tttcttgatt tctttacggc gatgatcttc gcctgctcgc tgggtattgc
    79321 ggtgtcggta attagtatcc cattactgat catccagtta acgctggcgt gggctgccgc
    79381 gctgatatta ccgctgacca caccgtcgat gatggcagac ttcagcgccg taggcggttt
    79441 attgctgctg gcaaccggat tacgcatctg tggcattaaa atgttcccgg tggtcaacat
    79501 gcttccagca ctccttctgg caatgccgct ttccgccgcc tggaccgcct ggtttgcctg
    79561 acaatgcgtg caatagcggc aaagtgatga tagattgtgc agtctgcagt aaattgaaga
    79621 aatttgattg acgagacgag gcgaatcagg tttaatgcgc cccgttgccc ggatagctca
    79681 gtcggtagag caggggattg aaaatccccg tgtccttggt tcgattccga gtccgggcac
    79741 caaattcata tcaacggacc tccacggagg tccgtttttc gtttcagaac accatgattt
    79801 aagtgttctt cctccaaatc aattctaccg aactcaacca aattctcccc acatcaacct
    79861 cattgtgtgg gtataattgc gggtataccc cagttcgaca gaatttatac cctctttaac
    79921 gcattcagaa ggctgaacta tggcactgac tgacgcaaaa atccgggctg caaagcccac
    79981 tgacaaggct tataaactca ctgacggggc cggcatgttc ctgctggttc atcctaatgg
    80041 ttcccgttac tggcgtctcc gctatcgtat tctgggtaag gagaagactc tggcacttgg
    80101 tgtgtatcca gaagtttctc tctccgaagc tcgtacaaaa cgggatgagg cccgaaaact
    80161 gatttcagag gggattgacc cttgcgaaca gaaaagagct aaaaaagttg tccctgattt
    80221 acagctctct tttgaacata ttgcacgacg ctggcatgcc agtaataaac aatgggcaca
    80281 atcacacagc gataaagtac tcaaaagcct cgagacacac gttttcccct ttatcggcaa
    80341 ccgggatatc acaacactca
//
LOCUS       DAHBHC010000012        76223 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHBHC010000012 DAHBHC010000000
VERSION     DAHBHC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.012"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(27..953)
                     /locus_tag="M2A35_001066"
     CDS             complement(27..953)
                     /locus_tag="M2A35_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCB7197396.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(1098..2447)
                     /gene="lysC"
                     /locus_tag="M2A35_001067"
     CDS             complement(1098..2447)
                     /gene="lysC"
                     /locus_tag="M2A35_001067"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCB7197397.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            2972..4621
                     /gene="pgi"
                     /locus_tag="M2A35_001068"
     CDS             2972..4621
                     /gene="pgi"
                     /locus_tag="M2A35_001068"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCB7197398.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4976..5218
                     /locus_tag="M2A35_001069"
     CDS             4976..5218
                     /locus_tag="M2A35_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197399.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            5332..5970
                     /locus_tag="M2A35_001070"
     CDS             5332..5970
                     /locus_tag="M2A35_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCB7197400.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5967..6704
                     /locus_tag="M2A35_001071"
     CDS             5967..6704
                     /locus_tag="M2A35_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCB7197401.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAASRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            6704..8800
                     /locus_tag="M2A35_001072"
     CDS             6704..8800
                     /locus_tag="M2A35_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCB7197402.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYEHGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLITGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRRVNFR"
     gene            complement(8847..9125)
                     /locus_tag="M2A35_001073"
     CDS             complement(8847..9125)
                     /locus_tag="M2A35_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197403.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            9395..9805
                     /gene="psiE"
                     /locus_tag="M2A35_001074"
     CDS             9395..9805
                     /gene="psiE"
                     /locus_tag="M2A35_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCB7197404.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(9849..11324)
                     /gene="xylE"
                     /locus_tag="M2A35_001075"
     CDS             complement(9849..11324)
                     /gene="xylE"
                     /locus_tag="M2A35_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCB7197405.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLVSMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(11696..12586)
                     /gene="malG"
                     /locus_tag="M2A35_001076"
     CDS             complement(11696..12586)
                     /gene="malG"
                     /locus_tag="M2A35_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCB7197406.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(12601..14145)
                     /gene="malF"
                     /locus_tag="M2A35_001077"
     CDS             complement(12601..14145)
                     /gene="malF"
                     /locus_tag="M2A35_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCB7197407.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(14299..15489)
                     /gene="malE"
                     /locus_tag="M2A35_001078"
     CDS             complement(14299..15489)
                     /gene="malE"
                     /locus_tag="M2A35_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCB7197408.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAACGRQTVDEALKDAQT
                     RITK"
     gene            15854..16969
                     /gene="malK"
                     /locus_tag="M2A35_001079"
     CDS             15854..16969
                     /gene="malK"
                     /locus_tag="M2A35_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCB7197409.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            17041..18381
                     /gene="lamB"
                     /locus_tag="M2A35_001080"
     CDS             17041..18381
                     /gene="lamB"
                     /locus_tag="M2A35_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCB7197410.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            18624..19544
                     /gene="malM"
                     /locus_tag="M2A35_001081"
     CDS             18624..19544
                     /gene="malM"
                     /locus_tag="M2A35_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCB7197411.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            19773..21353
                     /locus_tag="M2A35_001082"
     CDS             19773..21353
                     /locus_tag="M2A35_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCB7197412.1"
                     /translation="MISHIPNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLNRDN
                     FIYISNIFLNIKQRNEKNHAINMFREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDMNLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGYCNFTTATVDNVIFRCRR
                     LHNVIFIKASGECVDFSKNILDTVDFSRSQLTHSNFRECQIRNSNFDHCYLYASHFTR
                     AEFLSDKEISFIKSNLTAVMFDHVRISTGNFKDCITERVELTIDYSDIFGNEDLDGYI
                     NNIIKMIDTLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPII
                     RSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQ
                     ALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIFILFSSA
                     DDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            21576..22073
                     /gene="ubiC"
                     /locus_tag="M2A35_001083"
     CDS             21576..22073
                     /gene="ubiC"
                     /locus_tag="M2A35_001083"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCB7197413.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            22086..22958
                     /gene="ubiA"
                     /locus_tag="M2A35_001084"
     CDS             22086..22958
                     /gene="ubiA"
                     /locus_tag="M2A35_001084"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCB7197414.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(23113..25536)
                     /gene="plsB"
                     /locus_tag="M2A35_001085"
     CDS             complement(23113..25536)
                     /gene="plsB"
                     /locus_tag="M2A35_001085"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCB7197415.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            25707..26075
                     /gene="dgkA"
                     /locus_tag="M2A35_001086"
     CDS             25707..26075
                     /gene="dgkA"
                     /locus_tag="M2A35_001086"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCB7197416.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            26185..26793
                     /gene="lexA"
                     /locus_tag="M2A35_001087"
     CDS             26185..26793
                     /gene="lexA"
                     /locus_tag="M2A35_001087"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCB7197417.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            26866..28191
                     /gene="dinF"
                     /locus_tag="M2A35_001088"
     CDS             26866..28191
                     /gene="dinF"
                     /locus_tag="M2A35_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCB7197418.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            28307..28516
                     /locus_tag="M2A35_001089"
     CDS             28307..28516
                     /locus_tag="M2A35_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCB7197419.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(28558..29073)
                     /gene="zur"
                     /locus_tag="M2A35_001090"
     CDS             complement(28558..29073)
                     /gene="zur"
                     /locus_tag="M2A35_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCB7197420.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            29391..30376
                     /locus_tag="M2A35_001091"
                     /pseudo
     CDS             29391..30376
                     /locus_tag="M2A35_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            30739..30861
                     /locus_tag="M2A35_001092"
     CDS             30739..30861
                     /locus_tag="M2A35_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCB7197421.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDRMYRC"
     gene            30990..31523
                     /locus_tag="M2A35_001093"
     CDS             30990..31523
                     /locus_tag="M2A35_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197422.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            31720..31893
                     /locus_tag="M2A35_001094"
     CDS             31720..31893
                     /locus_tag="M2A35_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197423.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(31941..32222)
                     /locus_tag="M2A35_001095"
     CDS             complement(31941..32222)
                     /locus_tag="M2A35_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HCB7197424.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPI
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKGKEHH"
     gene            complement(32259..32831)
                     /locus_tag="M2A35_001096"
     CDS             complement(32259..32831)
                     /locus_tag="M2A35_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCB7197425.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYTVVSL
                     DSAMEQGATPDGDTILWWLKQSPEARAAICIDDTLSISDALSELSHFINRHADNTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            complement(32831..33142)
                     /locus_tag="M2A35_001097"
     CDS             complement(32831..33142)
                     /locus_tag="M2A35_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197426.1"
                     /translation="MSNYLYWSGLVANIALMLFVALCIWVWFIWPFVEAMSITRCFIC
                     ASKTSGCKPTVRAIIRTLKYWYLDLLFGRGWTRISNRQFEWEGVGNWRIHSSKETQEV
                     K"
     gene            complement(33139..33525)
                     /locus_tag="M2A35_001098"
     CDS             complement(33139..33525)
                     /locus_tag="M2A35_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HCB7197427.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALASLEEEPVA
                     WLHSDNGLGIPAITRSKNVADSWLSKGWYVQPLYIAQPVHVPEEMNLARAQKEVGFNR
                     YIMAGYVDGWNACRAAMLQSQGGGNQ"
     gene            complement(33525..33887)
                     /locus_tag="M2A35_001099"
     CDS             complement(33525..33887)
                     /locus_tag="M2A35_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197428.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NFNITHWAVMPKAPEAGA"
     gene            complement(33878..34414)
                     /gene="yfdR"
                     /locus_tag="M2A35_001100"
     CDS             complement(33878..34414)
                     /gene="yfdR"
                     /locus_tag="M2A35_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCB7197429.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELRKCA"
     gene            complement(34542..35366)
                     /locus_tag="M2A35_001101"
     CDS             complement(34542..35366)
                     /locus_tag="M2A35_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCB7197430.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSNVETFIGTFTA"
     gene            complement(35432..35794)
                     /locus_tag="M2A35_001102"
     CDS             complement(35432..35794)
                     /locus_tag="M2A35_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197431.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            36658..36867
                     /locus_tag="M2A35_001103"
     CDS             36658..36867
                     /locus_tag="M2A35_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197432.1"
                     /translation="MDNQNTPVFDVCGWYCVPVKDHNSVIVQLSCKQPPFESDKIIES
                     PIYSFPIKEIPNLINTLQNIYEENN"
     gene            complement(37063..37737)
                     /locus_tag="M2A35_001104"
     CDS             complement(37063..37737)
                     /locus_tag="M2A35_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCB7197433.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKKITLVPVISWVQAGA
                     WKEVGYSEVDLSTTETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            37828..38028
                     /locus_tag="M2A35_001105"
     CDS             37828..38028
                     /locus_tag="M2A35_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7197434.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            38072..38623
                     /locus_tag="M2A35_001106"
     CDS             38072..38623
                     /locus_tag="M2A35_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197435.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPELEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            38620..39771
                     /locus_tag="M2A35_001107"
     CDS             38620..39771
                     /locus_tag="M2A35_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7197436.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEGFMSMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKTGAGIGVLKLITEHNRAHAVFSCHEHCYAQIMVGRT
                     GPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMTTLPTLSQPEIAIVD
                     GQAVTSSLAVADFFSKRHDDVLKKIRTLECSASFTARNFSVSDYTDCTGRKLPCYQIT
                     RDGFAFLAMGFTGKRAAQFKEAYINAFNQMEKQLSKPSVPSDVAHNASVLYSYISSIH
                     QVWLQQLYPMLEKAESPLAVSLYDRINDAAFLARLIHSSLNSSEVRGRK"
     gene            39768..39992
                     /locus_tag="M2A35_001108"
     CDS             39768..39992
                     /locus_tag="M2A35_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197437.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            39989..40807
                     /locus_tag="M2A35_001109"
     CDS             39989..40807
                     /locus_tag="M2A35_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7197438.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            40804..41298
                     /locus_tag="M2A35_001110"
     CDS             40804..41298
                     /locus_tag="M2A35_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7197439.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSADIADAFADYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQELEPQPVRETRPVRNFCVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLQKSSKRAASGEEWYLSGN
                     YVGA"
     gene            41298..41951
                     /locus_tag="M2A35_001111"
     CDS             41298..41951
                     /locus_tag="M2A35_001111"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCB7197440.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            41948..42274
                     /locus_tag="M2A35_001112"
     CDS             41948..42274
                     /locus_tag="M2A35_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCB7197441.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            42271..42666
                     /locus_tag="M2A35_001113"
     CDS             42271..42666
                     /locus_tag="M2A35_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7197442.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFACKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPGGKVEITISKYEKVSCKLATR"
     gene            42818..43633
                     /locus_tag="M2A35_001114"
     CDS             42818..43633
                     /locus_tag="M2A35_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCB7197443.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGESRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            43641..44630
                     /locus_tag="M2A35_001115"
     CDS             43641..44630
                     /locus_tag="M2A35_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7197444.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSNWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHNELHADTVAFEEKYGSQLELIFRFIDRALATGVLA"
     gene            44644..45396
                     /locus_tag="M2A35_001116"
     CDS             44644..45396
                     /locus_tag="M2A35_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7197445.1"
                     /translation="MNLEALPKYYSPKSPKLSDDALATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            45783..45858
                     /locus_tag="M2A35_001117"
     tRNA            45783..45858
                     /locus_tag="M2A35_001117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45816..45818,aa:Ile,seq:cat)
     gene            45860..45936
                     /locus_tag="M2A35_001118"
     tRNA            45860..45936
                     /locus_tag="M2A35_001118"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45894..45896,aa:Arg,seq:tct)
     gene            45939..46014
                     /locus_tag="M2A35_001119"
     tRNA            45939..46014
                     /locus_tag="M2A35_001119"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45972..45974,aa:Thr,seq:tgt)
     gene            46018..46092
                     /locus_tag="M2A35_001120"
     tRNA            46018..46092
                     /locus_tag="M2A35_001120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46051..46053,aa:Gly,seq:tcc)
     gene            46420..46914
                     /locus_tag="M2A35_001121"
     CDS             46420..46914
                     /locus_tag="M2A35_001121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197446.1"
                     /translation="MRNVSFDVRYSFHLETMMATLYRRLEYLLYFILMLAGGLVLADV
                     GNTALQGLVVIIVAFTSLTLRPARNALLCERQAARYRELMHDFEFHPDETELPDMDEI
                     ELHDNTAIGLLRNAAYKRTLIVLGYSQEANAIHLTMMEKIAAWFAGDLPEECVPAGAV
                     DNEI"
     gene            47214..47759
                     /locus_tag="M2A35_001122"
     CDS             47214..47759
                     /locus_tag="M2A35_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7197447.1"
                     /translation="MNYPDIVNGIDFRDLVMVAGDSVLTTSVKVAEFFGKSHKNVLRK
                     IRQIISECPDDFARLNFEPTDFIDKNGDIQPMFNMTKDGYMLVVMGFTGKAAMKMKVA
                     YIQAFNWMAELIRQGVANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLQAR
                     IEQLEQRGQISLPGIDSVLTH"
     gene            complement(47978..48472)
                     /locus_tag="M2A35_001123"
     CDS             complement(47978..48472)
                     /locus_tag="M2A35_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003841965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197448.1"
                     /translation="MDKFDRQTQLEFLSSLNDIFPDKIRNNEQLKRLLSVFPDNKTAI
                     ANLLYLEGHGLITSGLRLDSCGYSHVWMPAITINGIDFLRNDGGLSAILKVQTIKFHH
                     STLTAIEDIIRIANIPEDQKKGLISKLRELPSDAIKHLTLQLLTQGVMNIPAALPLIE
                     KALR"
     gene            48611..49183
                     /locus_tag="M2A35_001124"
     CDS             48611..49183
                     /locus_tag="M2A35_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197449.1"
                     /translation="MKTFICTASGPSLSAEDCRFIADCGLPVIAVNSTWKRVPFATHV
                     FACDFSWWKACVRHITIPAQRWTDSRAAHKRFGINFFPEERGSPVNSGRRAIEFALWL
                     GAERVILLGYDCSVSRGTHWHGDHPEGLKNPTENSTRLWRSEFEKHAPVLNARAEIIN
                     CSVETALTCFPRRRLREVLDATVKKSPRVC"
     gene            49149..50081
                     /locus_tag="M2A35_001125"
     CDS             49149..50081
                     /locus_tag="M2A35_001125"
                     /inference="COORDINATES: protein motif:HMM:NF013257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197450.1"
                     /translation="MPLSKSRPVYVKGMHGIGDNINQRMFIDALSRAGYRVELQTPLP
                     QLYADLPGVTFIPSETTLRTQQKNEQRAYRSLPYIARPSADAQRIRIMHDRQTKGRGE
                     SVVMTMTRQFGIAPAPASLPSFRRSSLVVTDKPVAVIRPTTVRNEWKTTSRGPLNEYI
                     DMASRMLAARGYHCVSVADLNESDACEKRREWIDGMVPFAHQRFHHGELSLEELLLLT
                     ESAAVVVCGDCLLLHAAIAYGTPLLAILGGMGGACRPEIIADPSHYDLSKVCFIKPDN
                     FCLCHEMTHDCDKHISGVEGKIAAFLDGPVFRGG"
     gene            50083..50403
                     /locus_tag="M2A35_001126"
     CDS             50083..50403
                     /locus_tag="M2A35_001126"
                     /inference="COORDINATES: protein motif:HMM:TIGR01594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCB7197451.1"
                     /translation="MPDKSPGFWMSLLAWFRAEPDVSVAAATGLMALLRSCQQGKSLR
                     AMLLDSCICAFLAFYLRDALGLCGVDQDWAMILGVVLGYLGTEFIRTLLIRWAERRAG
                     ITRH"
     gene            50417..50884
                     /locus_tag="M2A35_001127"
     CDS             50417..50884
                     /locus_tag="M2A35_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HCB7197452.1"
                     /translation="MNKNVKAFLDALAWTEGTSTSRYTKNDGYDIIVDGIDSPHTFTD
                     YSTHPAVLVTVNRRGLKSTAAGRYQLLSRYWPHYRDLLKLPDFSPASQDAVAVQMIKE
                     QGAYADVVAGRTEIAVQKCRNIWASLPGAGYGQREVTMAMFVAQYRRCGGVTA"
     gene            50881..51117
                     /locus_tag="M2A35_001128"
     CDS             50881..51117
                     /locus_tag="M2A35_001128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197453.1"
                     /translation="MSVIPQWRYWWRFYSTHALLIASAVPLAMNEAESYLGQSLPFWL
                     KLLVALVILVSGVTGRLIVQVKEGENVDTAQRTD"
     gene            51089..51370
                     /locus_tag="M2A35_001129"
     CDS             51089..51370
                     /locus_tag="M2A35_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197454.1"
                     /translation="MWTLLSALISAIAAFFGARKADTAQSEKQLINRTSRTGADITRK
                     TQRKTEREIREATDISAADSGRVRASGSVSERTGIINDAIRRVNDEGHR"
     gene            51291..51584
                     /locus_tag="M2A35_001130"
     CDS             51291..51584
                     /locus_tag="M2A35_001130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197455.1"
                     /translation="MLLAACQNAPESLTTQSDGLTTKATVNCGEDTPFHTLPSYPECS
                     ECDQTDDPAVLRDYIAAQQAWAVDVIGVHTWNSILRDNTAGCLRQLRDNGIIN"
     gene            51607..51957
                     /locus_tag="M2A35_001131"
     CDS             51607..51957
                     /locus_tag="M2A35_001131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197456.1"
                     /translation="MSVSLSTLTTIVSGVSAIASGVVSVYENGAKLLDIMKSAIVKVE
                     EAYEALKASGATKKALVLATLKAAAESLGEEWDNICAYFSALIDSVVDLYNTAVAAIS
                     GTTYEDATGEEVTA"
     gene            52003..52434
                     /locus_tag="M2A35_001132"
     CDS             52003..52434
                     /locus_tag="M2A35_001132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197457.1"
                     /translation="MPKITLTVEDTIRIVLNNDDVLSLVKQKYLESTPPEFDLSELEA
                     LVSDTSDDDEPEADESETDTDSLALSETTVSGKAGDTLTVQVTAAGDSDVQVNTSDLS
                     VATAEYADGVLTITLVAEGTATIALTKGESSAEVAVTVTAD"
     gene            52508..52690
                     /locus_tag="M2A35_001133"
     CDS             52508..52690
                     /locus_tag="M2A35_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197458.1"
                     /translation="MRKYKRILLRLTMKNGLELKAPVTDDISRAVAFAIKWVAVGIAV
                     SPMLYGLAKLIVALKS"
     gene            52725..52868
                     /locus_tag="M2A35_001134"
     CDS             52725..52868
                     /locus_tag="M2A35_001134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197459.1"
                     /translation="MIKAETTPQGETEGYPSAVTVTGNTRLVQTDTSGRTWRVMGEDG
                     TSL"
     gene            53049..53492
                     /locus_tag="M2A35_001135"
     CDS             53049..53492
                     /locus_tag="M2A35_001135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197460.1"
                     /translation="MNNKENVKFTSELICGAMTHQIAGITDRVRSRAFWECLLREAPE
                     ELLPLLISRAIIRGRVFMAAGDIRFPVEPEPKADMFADFREELEKAEKRELMCIALKS
                     QLSGEVDEVRITEFWKAFLRLSSRELLAEVLTEVIHECSKKVRCG"
     gene            53485..54150
                     /locus_tag="M2A35_001136"
     CDS             53485..54150
                     /locus_tag="M2A35_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197461.1"
                     /translation="MADLNDLHRQLQGMQKQVRFATARAITSTVRKIEAAEKENLRRK
                     LDNPTPFTVNSVRSKGATRDNLTGRVFIMDTAVPYLEPFEVGGLHYLGEGQKAVLNPK
                     NIRLNKYGNLPKAKLQQLKARPDVFIGKVMNNDGEAVGGVWQRKKAKKVAKTRKRRKR
                     SPNGTRAPRKKNRPPKLLIRFGDALPVTPVLGYQELAHDMASRLMKTELNVALEQALK
                     TAK"
     gene            54472..55059
                     /locus_tag="M2A35_001137"
     CDS             54472..55059
                     /locus_tag="M2A35_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HCB7197462.1"
                     /translation="MTVGQYAEHAGVNRKTISRWIKAGTFVVMDGKKIDVEATDKNLK
                     RFRDSADPRAANGKKNRVARKARESQSGTVEGFEQRARDVFDSLTSGDVVIRPLEESR
                     AMKEHFFAEMARLEYEDKDGKLFPLKLVTEQVAAEYTRVRTHLVALAPEHGPRLRALA
                     GMTDDQGFTAALQELVYEALDELAFEKREIEEDPS"
     gene            55013..56992
                     /locus_tag="M2A35_001138"
     CDS             55013..56992
                     /locus_tag="M2A35_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7197463.1"
                     /translation="MNLHLRKGKSKKTLLKKALIQLQRHLASPQPMSLSQWANTFAVL
                     SRETSAQTGKFRSYPYQDGMMDAITDPSVTYVSVMKSARVGYTKILDHVVGYYLAHDP
                     SPILVVQPRVEDAEDYSKTEIAPMLRDTPVLAAISGDPKAKNSNQTILRKTFSNGANL
                     TLVGANSPGGFRRITCRIILFDEVDGYPAGGAGSEGDQIALGTKRSETFWNRKIVLGS
                     TPTVKGVSRIEKAWLESDQRRYFVPCPHCGEYQVLEWGSKSTPYGIKWEKDSEGNGLP
                     ETAYYLCRHHGCVIVHSELPGMLAKGEWRAGAPFRGHAGFHIWTGYSPHSNASWPNLV
                     AEWLRVKDDPLMRQTFINTTLGEPYEDAGEFAMSEQRLIARVEVWGAEVPEGVLLLTA
                     GVDTQDDRFEITVLGWGMNEECWVIAHDVIFGDLETEEPWERLDAYLKQVWRRADGFG
                     LTLSAVCHDSGGHHTNKVYEFSKARIGRRIWATKGESATGGKRNPVWPTRVVSSRNRK
                     SFRPVILGVNSAKDDIRHRLHIEPDPAGAPTAGCIHFPSFLDLHYFSQLLSERLVRKE
                     SGGQVYRVWELSSGRANEALDCMVYGYAALKGLLHHGLKLNRLAERTAQDLSFMAPPP
                     AQPEEKISLSMPGAKAPETPRKKIPLHMRLAGVRR"
     gene            56989..57240
                     /locus_tag="M2A35_001139"
     CDS             56989..57240
                     /locus_tag="M2A35_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCB7197464.1"
                     /translation="MMNTPGVFAGMSKEQLKAALNEAQAAYIELLSGRRGVSFSYAQG
                     DGTRTVTYSQASSADLLALIATLQRALGIRTRRSLSVRY"
     gene            57249..58889
                     /locus_tag="M2A35_001140"
     CDS             57249..58889
                     /locus_tag="M2A35_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7197465.1"
                     /translation="MIVDKNGRPFPQQTEKKRALNDSGRIPYDSAGFSHGSVAGWNPV
                     LWSPDNEVNIWRNRMVARMRDLVRNDGWANGSITRLLDNAMGVVFRPRMKPDYRMLAE
                     MTGNRAFDADWADEYGRCVEAHWRNWAGDAGCYCDLERRQTLPQLFRLAFRHKMVDGD
                     ALAVIHWRPDRIAPGRGRYGTVVQVIDPDRLSNPNDAFDQPHIRGGVEIDADGVPVAY
                     HIRSAHMGDWWSGSDTMHWERVPRETSWGRPVVVHDFDHDRAGQHRGVGILNPVVQRL
                     KMLIKYDQVELEAAIINAMFGFFITSPYDPKLTEDLMSDAEVINGYQDARMKYHDTNR
                     ISMSGVRIPITFPGEEPKAVSAARPVSNFKEFEGTVLRNIAAALGLSTQQVTQDWSDV
                     NYSSARAALLEAWKTLTRRRDEFAVGFAQPVLTAFLEELHEEEDLPLPASAPDFLAAR
                     AAYTRAQWMGPGRGWVDPVAEKKGAILAMEAGMSTLEIEVAENVGEDFEDILDQRARE
                     ERAFKERGLTPPSWFQAEQFAPTPTGSAPAEPKEPDVS"
     gene            58886..59746
                     /locus_tag="M2A35_001141"
     CDS             58886..59746
                     /locus_tag="M2A35_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCB7197466.1"
                     /translation="MNQLALLSQRLLNTPLALHPRKAEIIVTALAERLGITRIKTGAW
                     YDDDEDDFCRPARESGYDILEGIAIIPVHGTLVQKLGTLRPYSGMTGYDGIRRNFLTA
                     LNDPDVKGICLDIDSPGGEVAGCFDLVDTIFACRGQKPVHAILSESAYSAAYALASAA
                     DRILVPRTGGVGSVGVIYVHCDMSRQMKDEGLNVTIITCGSRKAETSPLRPLSEAAQA
                     ALQADIDAAGTLFIETVARNRGISADAVRALEARTLRADEGVQAGLADDVMSPDEAFS
                     FLLKETANNG"
     gene            59739..60320
                     /locus_tag="M2A35_001142"
     CDS             59739..60320
                     /locus_tag="M2A35_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197467.1"
                     /translation="MGKFSFMHLVGMGGRSRMDDDPEDKRDDENAGDDEDRRESRKAR
                     SRADDDDNDDGENAGDDEERRESRKARSRADDDDNDDDENAEDDDDEEKIRASERHRC
                     AAIFATPYAAKNTALAAELAFNTRMSVKQASAVMKAAVAVSSGQKARLAERMQHVPQP
                     GTGRDVRPAPTEAHAMAQHAMKLYNEAMGGKAQ"
     gene            60317..60718
                     /locus_tag="M2A35_001143"
     CDS             60317..60718
                     /locus_tag="M2A35_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCB7197468.1"
                     /translation="MSTYGNNPSVPGYWSDTYQPDQLLCGPLQVVTKTVTITGGEVYQ
                     RGTVLGRITESGAYTLCTQGTGVTDGSETPVAILADLADASSGDVLAGVYLMGEFNAN
                     RVIFDESWDIDDLSVALEKEKIFLRNPVTVP"
     gene            60774..61829
                     /locus_tag="M2A35_001144"
     CDS             60774..61829
                     /locus_tag="M2A35_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCB7197469.1"
                     /translation="MSEKDFNLLYDTAFLAQVVPNLFVAQNWLLDSFFPNIVLSDTES
                     VAIDVEVGARRMSPFCSPLVEGPMVEALPYQTNIFKPAYIKDKRVPDLLRPVRRMIGE
                     RLGGREYTPAERAMLNLQYEMADQINMLNRRLEWMAAQALQYAKVTIAGEGYPTTEVD
                     FRRDSDLTVTLSGDDVWPSEESSTIPTTCLEAWAMLMLKKSGAYPTEVIFTPSAWKAF
                     MNDSFIRENAINMPALNPTSNVVNPGTQINTGAVYKGKWGNFNLWLYNDWFIDPDDGT
                     EKPMLDDGNVILTGAALMGTRAFACIMDPAFNYGQMAYAPKMWDQQDPAQRFLMMQSA
                     PIVIPSRVNACLCASVV"
     gene            61831..62016
                     /locus_tag="M2A35_001145"
     CDS             61831..62016
                     /locus_tag="M2A35_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197470.1"
                     /translation="MASGKKGAPVDYVVLRGCVQHNGQRVAQGHVITLSESEAQRLLR
                     LGVIVSLAEMKGDDRTG"
     gene            62000..62335
                     /locus_tag="M2A35_001146"
     CDS             62000..62335
                     /locus_tag="M2A35_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197471.1"
                     /translation="MTGPVDWDKNLLSPLFGVFGEECEHRPRGGEAYRLTGVFDRAYT
                     QQLVGEDGGTESNSTLPVLGVRDAECRVKPKQGDIFLIVRTGKLYIVRDVQPDSHGGT
                     RLELNRVKE"
     gene            62332..62898
                     /locus_tag="M2A35_001147"
     CDS             62332..62898
                     /locus_tag="M2A35_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7197472.1"
                     /translation="MNTAEARKIVTRALSDATGAGWKVYSPRTLPVMPDQYPLVIVSV
                     QSEHKVSQGRHVPQYTTTTTLRIDGRVLAYDSGDETESAAGVAWEEAEAMKEAIERAV
                     IGNPAVRMKFQQIADIRAHIGVDSDGEAHTGIVVLELDLDLEYYQGPEDFFPSEIVPL
                     REVNVRGVHPSFRLHFDLPAGDETGSET"
     gene            62912..63136
                     /locus_tag="M2A35_001148"
     CDS             62912..63136
                     /locus_tag="M2A35_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCB7197473.1"
                     /translation="MFVKPVPGRRVRYPGDPSRLLPDEGAEVPDRDLFWRRRLKQGDV
                     VLADKATTAASGKVTVSTAATGSTAKGGDA"
     gene            63133..64635
                     /locus_tag="M2A35_001149"
     CDS             63133..64635
                     /locus_tag="M2A35_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCB7197474.1"
                     /translation="MTVSFSNIPSNLRVPLFYVEVDNSMANSATETQRTLLIGQMTAE
                     GTATAGTAYRCSSASTAAGLCGEGSMLHTMLTAYLKNDSYGETWLLPLADDDSSMTTA
                     TGSISVDSVPTASGVIYLYIAGTRVRLTVKSTYTQSEIASLLAARINATSALPVIASV
                     ASDGTTIELTARNAGEVGNTIDIRLNYSGSSGGESTPDGLTLTITAMSGGEGAPDLAD
                     ALASLGDRTFDFIVLAYPDTTSLNDMKDFLSDDEGRWAWDKQIYGHAFTAASGSYGEL
                     ADKGESRNDRHMTIWGVYDGPNTSYDYAAAMVGALAQSVRNDPARPTQTLSVSGVLAP
                     PLASRFTLTERNTLLYSGISTFTVSDDDTVTLENTITTYQTNSYGAADDSYLQIETMY
                     TLMYVMRSMKTQVTSKMGRMKLADDGANIPAGSAIVTPSIIRAELIAQFNTLANNGYV
                     QDADAFAEQLVVERDSDNPNRVNVVWPGRLMNQLRIFAVRNQFRLNSSSD"
     gene            64678..65040
                     /locus_tag="M2A35_001150"
     CDS             64678..65040
                     /locus_tag="M2A35_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCB7197475.1"
                     /translation="MSKNALAGTCTVTIDGVSVNVAGTFRYSVGEIERETLTGMSGIH
                     GFKESYKAPFIEMTVRDSGSLSLKDFAAYTDVTVAAYLVNGKTILGQNMWLVGRIETD
                     NNDATFTARFEGQTVSES"
     gene            65040..65321
                     /locus_tag="M2A35_001151"
     CDS             65040..65321
                     /locus_tag="M2A35_001151"
                     /inference="COORDINATES: protein motif:HMM:NF021614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCB7197476.1"
                     /translation="MMNEKQTEELVVTLKRPVRDPDTRTEFRELRLKEPVLFQAEDFH
                     RNTASLGAMAAMRELIAAVAGVPSAPLKFMAVSDYKKCERFLTGFFLEA"
     gene            65457..67424
                     /locus_tag="M2A35_001152"
     CDS             65457..67424
                     /locus_tag="M2A35_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCB7197477.1"
                     /translation="MAGNAFNFELNARDNASALLQRASEAAGSLADNAEQAAACVSSL
                     CDGLDAVNETPLSGASGAADALGGKITQIHDSVTLLMNALLATDSAGKRALGKESQEN
                     ADRMSGYFERLSRLGKDTSQHFGDIVPPLRNVGALSSELFSALGRGGLVGLTAAVGGK
                     AVKAVVSHLYDASKAAYSLDVNARNAGMSVSAFSRFAGVFRLMGLSAEQAQAETGALF
                     TTLNDALNTRSPEVVGILNQFGVKLAENSNHTVNLEKSTQNLTDAFGRMNSSSQKVVA
                     DALGLSDAQLALLRGTKNLNAALAESDRLKLTMPDSLNAKLVQANSNLNLLSAAWDGF
                     TDRVKAKVLGSDAVTASVDWAVDVMSDRRSSQQKTGDHLSELRSYLYAHPEKLKSLTS
                     RQRYNLDNNIATSDLLEMYTRLSGQRSDRLNALTATLSDDLAAALSAPSQAISQPPSS
                     SPQGGTGHNDITPELLAHFAALEKQYNLPANTLYGLAMTESSGRADAVGPLTRYGAAK
                     GMFQFIDPTAREFGLSGMDVFNPYRASEAAARKLAGLMAQYGGDMQRAFQAYNWGEGN
                     MSAWLRGHKGMPQETQAYAPKVLRHMSQISGGSIITPQSGSSVADVRDEISRGFSENK
                     IRLDINVTNTTTGQTTRRTVKGGAVSTAMDV"
     gene            67435..68847
                     /locus_tag="M2A35_001153"
     CDS             67435..68847
                     /locus_tag="M2A35_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCB7197478.1"
                     /translation="MGIITDAVADTLGLSSTGGWDWQSHINQASFRGVPFGVISGESV
                     VGRRVAVHEYPYRDTVWVEDLGRSARKFTLRGFLIQDSLVYSAGDVFSQRDAMIAACE
                     TSGGGLLVHPTLGEMTVYVPDGGLRIEEGVESGRVFSFTLTVIESGEKAFSLVTGTSS
                     TSSETWYQTLTTTAAVMLAAITGEMNSVTGAVKTIKSTVSAWETIFSRAVTSVTSMTS
                     TVSSLFSPDSYGRYCTASDSGTASGSTASSLSTWLATTEADDDSILETIQACSVRDRS
                     AAESAIAALDDISSVSGAVSAIQAVITTLAEATGSDTEKIRVMAEIASAEDGTYYEGE
                     TACAISASVQAFIRTLGAGAMLWRLMQYTPQGYDDAVSVMRKARTVTEAVLLLLADAG
                     DDDSHDALNAQYTQFVTHWQTNYLSQQDVMKVTSRSPQPSLALANRLYQDAARADELV
                     QAASPVHPAFMPLSFTARNS"
     gene            68844..69932
                     /locus_tag="M2A35_001154"
     CDS             68844..69932
                     /locus_tag="M2A35_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197479.1"
                     /translation="MSTTTDDTLTLKVNNTVMSGWNDIRVTRGVERLPSDFELSLTDW
                     YPQEGYQLAPPGSSCTITIGDDRVMTGYVDQWVNTLTPQSHDIRVTGRGMCQDLVDCS
                     AWWENNMIKGGDALAIIRKLASVYGITVTTDVDSFTTVPDFVINWGESSQQIIDRICR
                     YEGLLYYDLPDGSLFLTRAGTASAASGVTQGVNLQKAEYTRSMNERFSEYTGLSVSVN
                     SISELSPASGYDSVMLATARDPEAASMRTRRHVTIVESTLMTTGCAQQAVNWEMNRRY
                     GRSMALNVTVDSWRDSAGVLWQPNTFVPVSIPALGADSLNWLVSEVTFSRDDETGTTA
                     SLVLMPPEAFSVQPYRFYSGVAGRDVSS"
     gene            69929..70510
                     /locus_tag="M2A35_001155"
     CDS             69929..70510
                     /locus_tag="M2A35_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCB7197480.1"
                     /translation="MNPVQVLFRRLVSLLSVGRVTAGDDSGVVQTVQVQSPSEVRSDT
                     PVLQQFGFSSVLPAGTDVVVMSLAGNRSSAAVVASGHQSYRINGLSSGEVVVYNQWGQ
                     YVRLGEDGIVVEASGQQVLVNSATTLEVTATDGVTLKTPSLKVTGDITDNCESNSTTL
                     KALREAYNDHTHPVSGVESGGSTVTSQATTETV"
     gene            70512..70949
                     /locus_tag="M2A35_001156"
     CDS             70512..70949
                     /locus_tag="M2A35_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCB7197481.1"
                     /translation="MSDITLFYDEEALHCDWSVGTGDMVCGNDLQTAILISLFTDRLA
                     DASDETDDGNRRGWWGDLEQDYRVGSRLWLLRRQKLTTQVALKAEAYALEALQWLKDD
                     GVVASLDVEAWIVPPDRLYMTIAYQRPDADTTEYQKFYQVWEV"
     gene            70951..72105
                     /locus_tag="M2A35_001157"
     CDS             70951..72105
                     /locus_tag="M2A35_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCB7197482.1"
                     /translation="MPFERPGLSELREKSRSYVTGQLDEAGALLRFSTPGILADAVAG
                     MTHLHYGYLDWIAQQCTPATATGEYLAAWGALKGIIRKAAEAAACDEVQFTGTVGSTV
                     SAGAVLNRADGYQYTLDEDVSIDSDGSGSGSITAVLPDPTDDSSGGGDDGNADAGTTL
                     TPDVTWSGIDSTVTMVTAATGGSDTEEEETYRQRVLYAYQNPPQGGAAADYVQWALEV
                     PGVTRAWCVNRALGFGTVGVYIMTDGDDEDNAGGFPDGTDGVATDEDWTDRKATGVQL
                     TVADYIRDYQPVTAVVYVMSPVARSIDFEIQGLSDATAALRAQVESAISEVLYNVDEL
                     DGSGVINLSDLYYAIADIEGTDGFILVSPDSNITLSQGELPVTGSITWAT"
     gene            72114..73283
                     /locus_tag="M2A35_001158"
     CDS             72114..73283
                     /locus_tag="M2A35_001158"
                     /inference="COORDINATES: protein motif:HMM:NF021583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCB7197483.1"
                     /translation="MSRFSHDDYTCAMVNLLPSGMAWNRLPDSVQYRLMRGLAQAYRQ
                     SDADACALITGAFPETADALTDEWYASLGLNDECGTQASTTDPAQARKFILAKLLSTG
                     GQSVAYFTELAATMGYSIRIREYRTPLCGFSWCGHLLSDDNRFNWTVVVAPQGDDVTT
                     SRAYLECLFRRYAPAQTLVTFEWQSSYSAVLSMAWDNSTRLLSGALMADEGVVVSGVT
                     VNVVIKSMSGQRYVATVSTDSDGGWSFEADDETFTNGSYTVYAQADVDMPDDVTVPVR
                     SALLNVRVQTQGEGDNGDDEPASDDTTQEPLVINRVMLQSGRTTQEPLIISRAHLQSG
                     YPTQEPLVINSAMLQSGYLTQEPLVINSAMLQSGYLTQEPVVINRLWLNFAVTNE"
     gene            73306..74370
                     /locus_tag="M2A35_001159"
     CDS             73306..74370
                     /locus_tag="M2A35_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197484.1"
                     /translation="MNSIILEGFRQIAADEALCDSYTSDNILSALNKNGVTLITEMSG
                     SVSSVCRTTQTINTVARTGLAFTTSSNLMLRCLLPDLSETAVQFGFRLSLGEGEKYSS
                     SPSHIGIGSASFTTPYPDEAASYYFEIFAAWNGSSTVSASLYCNRALVGSATLGVSSS
                     GTGAVSVRIGGGNNVFASGVSGIFMPGDMYCMTVPYGDNYDASPSLLGSIEVGYSPVT
                     AFTGGSATNSLSKDIVTGLNASDDDSGYLILNSSGEAAGVTFEDIDNTGGTLVAVQAA
                     VTLRESASPNNLLAWSVGCGSSRGDVISETEAESDTNSWTTVTQGFMSMPGDGSEFET
                     GALTFTANLYNRKKSEQETA"
     gene            74370..75161
                     /locus_tag="M2A35_001160"
     CDS             74370..75161
                     /locus_tag="M2A35_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7197485.1"
                     /translation="MRKISDITSYVDENDEFTDGEPATGVKPTPLLALWFNVIQRELV
                     KVVESAGLTLDPTDDTQLWQALSGYFASQENVNTTISALGTMASQNSDAVDITGGAIT
                     GTSLTGDLTGNADTATKLKTGRKIRTNLDSTSAVSFDGSANVTPGVTGTLPVSNGGTG
                     ATTASDARTNLELGDVATQSLSTLDARYLLTTGGVSAVRLGSVSSYSPPGNESSWTQN
                     LSSGNVLTGIIVQDTGSNSADNIGGIYYRQVQYCINGTWINAVSL"
     gene            75174..75713
                     /locus_tag="M2A35_001161"
     CDS             75174..75713
                     /locus_tag="M2A35_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCB7197486.1"
                     /translation="MINIKNFTPYIPENPPVAWASYLISEDGQDWYECQTQFAEDTYK
                     VAYGSDGIVRSISTDVSALFPLNMSVAEVESLPDGADIDGNWVLDGESVVARTLTAAE
                     WQAKAESQRSALISDAKATISLWQSELLLGIISDDDKESLTEWLSYIKALQGLDLSGV
                     TDEEGYNATVWPDEPGATA"
BASE COUNT        18287 a        18547 c        21310 g        18079 t
ORIGIN      
        1 ttatttcact ccatcagctc ttattattat tgtgtattcc cgctgatttc attctgtata
       61 agtgttgcaa tatcgtcatt cgttgccgct tgtgaactaa atatcatctt ctcacgcgaa
      121 tacaccgcaa acttatctga caggttcgaa gcaaacacct tgcgggcatt ttctgccagc
      181 aaaccggtga cgatatcatg gtggttttta tccacttttc gggcaatatt tcccgcctct
      241 ttgtccttac tataccgttc attggtgctt tcccagctta atgccgctga ggttgttttt
      301 atcatgactt ctgtttggta gcctgcatct ttgagttttt ttaatgtttg taacggcgtc
      361 gcggcattac gaaatgttcc ttcgacaaca atgttgagtt tactgactat cgctctctca
      421 attgcacgtt cgaccgtttc gccggaaaat ttcgctgtat gggtaacaaa atcatcccca
      481 taattttggt agatataatt aaaatcaggg tgtagaaatc gaaaatcatc accgttaata
      541 atcattatat tatcttttgt ctctcttttg gctaattcaa tcagagatga tttacctgct
      601 ccgggttgcc cacctaaaac aaaaccaatg ggatgctgtt gcggtgtgac ttcatgattt
      661 gtcagacgct gccaaataga atcaaaagat gctttatttt ctgaatgcgt agtattgttg
      721 aattcatgct gatattgagt tcttacctgc tgaaggagaa tcttaccttg tgtaaaatca
      781 acaagttcag cgggggtaga ataaagataa tcgcgatagc tgactaatcg cttgagagca
      841 tcgcctgaat cctgatgcga cgaaactgag caattatcaa tgatatttga taaaaacgcg
      901 atgcactgac tggctccgtt taaaagtgca tctttttcca ttaaagtagc cattaacact
      961 cctgttaaat gatatttttc ggtgattata atactgttct acaggagtga ttttgctttt
     1021 cagatattat ttaatctatg ttttcagtga ggatggcgta taacaatcgg tgagcgttat
     1081 acgccagtag ggaaatatta ctcaaacaaa ttaaaatgca gcttctgcac cacctgctcg
     1141 gcatcttcgc cgggcaccag gaagcacagg ttatggctgg atgcgccgta acaaatcatg
     1201 cgaatgttga acggttccag tacgccgaat acctctttgc caacgccgca ggcttttgac
     1261 aggtcattgc caatcaacgc gaccagcgcc agaccttctt ctacttccac ccggcacagt
     1321 gcggaaagct ccatcagcag agattgcgtc agcaacgtat cgccagtgga ggttgaaccg
     1381 gtggtatcaa gggttaatgc cacgctcact tctgacgtgg tgattaagtc taccgaaata
     1441 ttatgccgcg cgaggatgcc gaaaacttcc gcgaggaagc cgcgagaatg cagcatattc
     1501 aggctgtgca aagtgagcag agtctgattg cgacgaagcg ccagcgcgcg gaacagcggc
     1561 ggattttcag ttttattgca caccagcgta ccacctgcgc gtgggtcttt gctggagccg
     1621 acaaagaccg ggatatcgct gcgtactgcg ggtagcaacg ttgccggatg cagtactttt
     1681 gcaccaaaag ttgccatctc tgccgcttcg gcaaacgcga tttcatcaat gcgttttgct
     1741 gcggaaacta cgcgtggatc ggtggtgtag atgcccggga cgtcggtcca gatatcaaca
     1801 cgagatgcgt gtaaagcctc cgccagcaag gctgccgtat aatcgctgcc tccacggcca
     1861 agcgtcgttg tacgaccttt attttcgcta ccgataaatc cctgggtgat cactaagccg
     1921 tcattgaggc gtgggagcag ctgcagcgcg gccagttccg ccagcgcggc tacatctggc
     1981 tctgcacgac caaatcggtc gttggtacgc atcactttac gtacatcaaa ccactgtgcc
     2041 tgaacatcgc gttcgcgcag gatttcgaca aacagcaggg tcgacatcag ctcgccgtgg
     2101 ctgaccagct catctgtcag cgccggagac gttgccagcg ccgccgcttc tgccagaaca
     2161 gtaatgttct ccagcagacg ttcaatctct tcacggataa cgttcgggta acgcaaacgt
     2221 tccagaatgg caaactggat gttgcgaata gcgtcgagtt tttcgaatcg ctcgccaggt
     2281 tccagccctt ccgctaaagc gaccagcaga ttagtgatac cagcagaagc cgagaggact
     2341 actaaacgca cgttggcatc agaaagcaca atatcagcgc tgcggttcat ggcgtcaaaa
     2401 tcagctacgc tggtaccgcc aaatttggag acaacaattt cagacataac tacctcgtgt
     2461 caggggatcc attttcagcc ttggcacaag ggaagagcgg aagacgggtg ggcgcagagc
     2521 gatacttcgc tactattttc acccagaagt gctccaccac ttgcgaaacg cccgactgcg
     2581 aacgcttctg gtgacaaccc aggggattca gcccctgtag ccgatgatga acgtggccag
     2641 ccgttcaatc acctcggcga tgcaccccct caggtgttat cacaggactg gctcctccaa
     2701 caccgttact tgggcaacgc gcctcttctg gcctgcgcta gcgcaggtag tacatttata
     2761 aataaagggt gagcggggcg gttgtcaacg acggggtcat gcggattttt catccactcc
     2821 tggcggtcag tagttcagct aataaatgct tcactgcgct aagggtttac actcaacatt
     2881 acgctaacgg cactaaaacc atcacatttt tctgtgactg gcgctacaat cttccaaagt
     2941 cacaattctc aaaatcagaa gagtattgct aatgaaaaac atcaatccaa cgcagaccgc
     3001 tgcctggcag gcactacaga aacacttcga tgaaatgaaa gacgttacga tcgccgatct
     3061 ttttgctaaa gacggcgatc gtttttctaa gttctccgca accttcggcg atcagatgct
     3121 ggtggattac tccaaaaacc gcatcactga agagacgctg gcgaaattac aagatctggc
     3181 gaaagagtgc gatctggcgg gcgcgattaa gtcgatgttc tctggcgaga agatcaaccg
     3241 cactgaaaac cgcgctgtgc tgcacgtagc gctgcgtaac cgtagcaata ccccgatttt
     3301 ggttgatggc aaagacgtaa tgccggaagt caacgcggtg ctggagaaga tgaaaacctt
     3361 ctcggaagcg attatctccg gtgagtggaa gggttatacc ggcaaagcaa tcactgacgt
     3421 agtgaacatc gggatcggcg gttctgacct cggcccgtac atggtgaccg aagcactgcg
     3481 tccgtacaaa aatcacctga acatgcactt tgtttctaac gtcgatggga ctcacatcgc
     3541 ggaagtgctg aaaaaagtaa acccggaaac cacgctgttc ctggtagcat ctaaaacctt
     3601 caccactcag gaaaccatga ccaacgccca tagcgcgcgt gactggttcc tgaaagcggc
     3661 aggtgatgaa aaacacgttg cgaaacactt tgcggcgctc tcaaccaatg ccaaagccgt
     3721 tggcgagttt ggtattgata ctgccaacat gttcgagttc tgggactggg tcggcggccg
     3781 ttactctttg tggtcagcga ttggcctgtc gattgttctc tccatcggct ttgataactt
     3841 cgttgaactg ctttctggcg cacacgcgat ggacaagcat ttctccacca cgcctgccga
     3901 gaaaaacctg cctgtactgc tggcgctgat tggcatctgg tacaacaatt tctttggtgc
     3961 ggaaactgag gcgattctgc cgtatgacca gtatatgcac cgtttcgcgg cgtacttcca
     4021 gcagggcaat atggagtcca acggtaagta tgttgaccgt aacggtaacg ttgtggatta
     4081 ccagactggc ccgattatct ggggtgaacc aggcactaac ggtcagcacg cgttctacca
     4141 gctgatccac cagggaacca aaatggtacc gtgcgatttc atcgctccgg ctatcaccca
     4201 taacccgctc tctgatcatc accagaaact gctgtctaac ttcttcgccc agaccgaagc
     4261 gctggcgttt ggtaaatccc gcgaagtggt tgagcaggaa tatcgtgatc agggtaaaga
     4321 tccggcaacg cttgactacg tggtgccgtt caaagtgttc gaaggtaacc gcccgaccaa
     4381 ctccatcctg ctgcgtgaaa tcactccgtt cagcctgggt gcgttgattg cgctgtatga
     4441 gcacaaaatc tttactcagg gcgtgatcct gaacatcttc accttcgacc agtggggcgt
     4501 ggagctgggt aaacaactgg caaaccgtat tctgccagag ctgaaagatg ataaagaaat
     4561 cagcagccac gatagctcga ccaatggtct gattaaccgc tataaagcgt ggcgcggtta
     4621 atttaaaaac cttaataaca atgccgacgt tatgtcggca ttgttttatc agataaatcc
     4681 ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa tcctaaaata gatttttaat
     4741 cgtgattgat ttcggaaaat actcagatta attactttta cttttatttt aacattatga
     4801 tttttaatgt tatttatata taagttgtag cttatatttg atattcattc caattatcct
     4861 aaaaccccat ctctaattcc ccgcgccgta attcgcatgc tttagttgtg tatactcgat
     4921 cccgcccgaa atgtttttgg gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa
     4981 aaaagttctg tatggcattt ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc
     5041 acctgtacag gtgggcgaag cggcagggtc ggcagcaacg tcggtttcgg cggggagttc
     5101 ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt gtcgcgctcg cggcaaccgg
     5161 tggcggtgat ggttctaata ccgggaccac cacaaccacg accaccagta cccagtaata
     5221 aagtatgtat ccccaaaata atttgagtca ttgcatctgt ggcttgaagt atgaagggat
     5281 ttaccataac cacactccgg tgtggttatt ctgcccctct ggagaagagt cgtgaagcga
     5341 cctgcactca ttcttatctg cctgctatta caggcctgtt cagccacgac taaagagctg
     5401 ggcaattcac tgtgggacag tctgttcggc acgccaggcg tacagctgac ggacgatgat
     5461 attcaaaata tgccctacgc cagccagtac atgcagctta atggcgggcc gcaattgttt
     5521 gtggtgctgg ccttcgctga agacggacaa caaaaatggg tcactcagga tcaagccact
     5581 cttgtcacac aacatggtcg tctggtgaag actttgcttg gcggcgacaa cctgattgaa
     5641 gtgaataacc ttgccgccga cccgctgatt aaacccgcac aaattgttga tggtgcaaca
     5701 tggacgcgca cgatgggctg gaccgagtac cagcaggtac gctacgccac cgcacgctca
     5761 gtcttcaaat gggatggcac cgataccgtc aaggtcggca gcgatgaaac cccggttcgc
     5821 gtgctggacg aagaagtctc caccgaccag gcgcgctggc ataaccgcta ctggatcgac
     5881 agcgaagggc aaattcgcca gtcggaacag tatctcggcg cggattattt cccggtgaaa
     5941 accactctca tcaaggcggc aaaacaatga ttaaacaaac tattgtcgca ttgattttga
     6001 gtgtgggggc gtcatcggtc ttcgcggcgg gaaccgtcaa ggtattcagc aatggcagca
     6061 gtgaggccaa aacgctgacg ggcgcagagt atttaatcga tctggtaggc cagccgcggc
     6121 tggcaaacag ttggtggcct ggtgcggtga ttagcgaaga gctggcaacg gcggcagcat
     6181 cgcggcagca gcaggcgttg ctgacacggc tggcagaact ggctgcagat tccagcgccg
     6241 acgatgccgc cgcgattaac gccttacgcc agcaaattca ggcgttgaaa gtgacgggca
     6301 ggcaaaaaat caatcttgat cctgatatcg tccgcgttgc cgaacgcggt aacccaccgt
     6361 tgcagggcaa ctacacgctg tgggtcgggc caccaccgtc cacggtcacg ctgttcgggc
     6421 ttatcagccg tcctggcaag cagccattca ctccaggtcg cgacgtggcg agctatctct
     6481 ccgggcagaa cctgcttagc ggtgcggatc gcagctacgc gtgggtggtt tacccggacg
     6541 gacgcacgca aaaagcgccg gtggcttact ggaacaagcg tcacgtagag ccgatgcccg
     6601 gcagcattat ttatgttggc ctcgcggact ccgtctggag tgagacgcct gatgccctta
     6661 acgccgacat tcttcagact ctgacgcagc ggatacccca ataatgaaaa aaagacatct
     6721 gcttagctta ctggcgctgg gcattagcac agcttgctac ggcgaaacat atcctgcgcc
     6781 cattggtccg tcgcagtcgg atttcggtgg cgtaggatta ttacaaacgc ccaccgcgcg
     6841 catggcgcgg gaaggggagt tgagcctgaa ctatcgagat aacgatcagt accgttatta
     6901 ctcagcttca gtgcaactct tcccgtggct ggaaacaacg ctgcgctaca ccgacgtgcg
     6961 cacccggcag tacagcagcg tcgaagcgtt ctctggcgat caaacgtata aagataaagc
     7021 cttcgatctc aaactgcgtt tgtgggaaga gagttactgg ctgccgcaag tggcggttgg
     7081 tgcgcgggat atcggcggta cggggctgtt tgatgcggaa tatcttgttg ccagcaaagc
     7141 ctgggggccg ttcgatttta cgctcggcct gggctgggga tatctgggca ccagcggtaa
     7201 tgtgaaaaat ccgctctgtt cagccagtga taaatattgc tatcgcgata acagctacaa
     7261 acaggcggga tctatcgacg gtagccagat gttccacggt cctgcctcac tgtttggcgg
     7321 cgtggaatac cagacgccct ggcaaccgct gcgcctgaaa ctggagtatg aaggcaataa
     7381 ttatcagcag gattttgccg ggaagctgga gcaaaaaagt aagtttaacg tcggtgcgat
     7441 ttatcgcgtt accgattggg ccgacgttaa ccttagctat gaacatggca acacctttat
     7501 gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc ccgtcctaca tcgataacgc
     7561 ccgcccgcaa tatcaaccgc agccgcagga tgccattttg cagcattcgg tggtggcgaa
     7621 tcagttaacg ctgttgaaat acaatgccgg acttgccgat ccgcagatcc aggcgaaagg
     7681 cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt gattcgcgcg aagggatcat
     7741 ccgtgctaat cggatcgtga tgaatgatct gccggatggg atcaaaacga tccgcgttac
     7801 ggaaaatcgc cttaacatgc cgcaggtgac gacggaaacc gatgtcgcca gcctgaaaaa
     7861 tcatctcgcc ggagagccgt tgggccacga aacgacgctg gcgcaaaaac gcgtcgagcc
     7921 agtggttccg cagtccaccg agcagggctg gtatatcgac aaatcacgct ttgatttcca
     7981 tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa aacttttaca tgtatcagct
     8041 gggcgtgatg ggaacggcag atttgtggct gacggaccat ctactgatca ccggcagcct
     8101 gtttgcaaat cttgccaaca actacgacaa gtttaactac accaatccac cgcaggactc
     8161 gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg cagaacgatg tctatgtgaa
     8221 taacctgcaa gccaactact tccagcatct gggcaacggc ttctacggtc aggtctacgg
     8281 tggttatctc gaaaccatgt ttggcggtgc gggggcagaa gtgttgtatc gcccgctgga
     8341 cagcaactgg gcgtttggtc tggatgccaa ctacgttaaa cagcgcgact ggcgtagtgc
     8401 aaaagatatg atgaaattca ccgactacag cgtgaaaacc ggacatctga ccgcctactg
     8461 gacgccatct ttcgctcagg atgtgttagt taaagccagc gtcgggcagt atctggcagg
     8521 ggataaaggc ggcacgctgg agatcgccaa acgctttgat agcggcgtgg tggtgggtgg
     8581 ctatgccacg atcactaatg tttcaaaaga ggagtacggc gaaggggact tcaccaaagg
     8641 cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc ccgacacgca gccgtgcggc
     8701 gattggctgg acgccgctga cgcgtgacgg tggtcagcaa cttgggcgta agttccagtt
     8761 gtatgacatg accagcgacc gtagagtcaa tttccgctaa gtcatgggaa aggtgccagt
     8821 tttcgcaatc actggcaccc tcattcttaa tggcctgttg agccatcagg aacgcagttc
     8881 acccccggtt tgcgatctgg tgtgtaaagg cgaaaacatt ctgtcttcct gtccgtcgat
     8941 atcggttgca gatctggttg cctgagcgac tgggccatca ttgggctgga gacattgctt
     9001 tgcggtaggt cggctttatc agcagtaccg agcggaccag cataagcagg aagaacagag
     9061 actgataaca tcaaagcagc aaaataaggc ttcattttta ccacctttat caggttacgt
     9121 ttcatttgtt ccagaggaac atggtcgatt tttcgcgcat tgctggtggc tgggaatcac
     9181 ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt tgtcttgccg gatgcgccgc
     9241 caggcgcggc ttatccggcc tacgggtagg tatatccggc tttggtggag gcgcgctcca
     9301 aatccaggtt gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     9361 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     9421 ggagtttatc tccaccattt tgcagaccgt actcaatctt ggcctgttgt gcctcggcct
     9481 gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gcagatgtgc tgttcgcgcc
     9541 agaacaaacc agcaaatatg agctggtaga aggactggtg gtttactttc tctatttcga
     9601 atttatcgcg ctgattgtga aatactttca gtccggtttt cacttcccgc tacgttactt
     9661 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     9721 ggatgtgttg atctactcgg ctgcgatcct gttactggtg atcactctgt ggttgtgtaa
     9781 ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg aactggcgcg gcgtgctgga
     9841 caggaagatt acagcgtagc agtttgttgt gttttcttcg tttccggttc ccagagggct
     9901 tccagctcct caagggtttt acctttggtt tccgggacaa atttccacat aaatagtgct
     9961 gccagaacgc ccatacaccc gtaaatccag taggagaaac cgttgtggaa atgggccacc
    10021 agccaggagt ttttgtccat catcgggaag gtccaggaga cgaagtagtt cgccagccac
    10081 tgggccgcca ccgcgattgc cagcgcttta ccacgaatgg cattcgggaa gatttccgac
    10141 agcagaaccc agcataccgg accccaggac atggcaaagg cggcaacata gaacagcatc
    10201 gacagtagcg ccacaatacc cggtgcctga gtgtaaaacg cggtaccgag gctaaacata
    10261 ccgattgcca ttccgagcgc gccgataatt tgcagtggct tacgaccaaa tttatccacc
    10321 gtcataattg ccagaacggt gaaggtgagg ttgataactc cgacaataat ggtctgcaac
    10381 agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt ccggcgcgta gtacaacacc
    10441 acattgatgc cgacaaattg ctggaagatg gagagcataa cgccgattac aatcacgccc
    10501 acgccaaaca tcagcagacg accaccggtt ttgcggccat gatccaggga gtgtttaatt
    10561 tcctgtactg cctgagttgc aagcgtgttg cccataattt tgcgcaggat actttccgcc
    10621 tgttcttgct tgccgcgtga catcagccag cgaggacttt ctggcacggt atacagcagc
    10681 attaagaaca gcagtgcagg gatacattcc gaggcaaaca tataacgcca gccgtcagta
    10741 ttcagccagc tggcatcacc ggaacgggca ataaaatagt ttacgcagta aactaaaagt
    10801 tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt tcccgcgaat atgagctgga
    10861 gccagttccg caatatacat tggtgaaagc attgagacta aaccaacgcc aataccgcca
    10921 ataatgcgat aaataacaaa ttccgggaca taacctgcca gataaacagg cactgtgttg
    10981 tccgggttta tagaggtaaa accaagttct ggccaggcag aacctacacc agaaataaaa
    11041 aacaggacag cagcaatctt aagtgaatca cgacgaccga agcggttact gcaataacca
    11101 ccgagggcac cgccgatgat gcaaccaatc agagcgctgg ctacgcaaaa ccctaacagg
    11161 gagttggcag cggattcatt taagttttgt ggagcaacaa agacggtatt gagtgactca
    11221 acagtaccgg aaataacggc ggtgtcgtag ccaaataata aaccacctaa tgtagcgact
    11281 aaggtaatcg aaaatatata actggaatta tactgggtat tcattcagac ctgccttaga
    11341 tcattctgat cttttatcag tgcattgatg ttggaattgt caggcagtta aggcaattat
    11401 cattttttac aatgcgttta cgtaatgtct tttctgtgat cttaattgtg ataattatcc
    11461 aaaattgata aaaacaatac tattgccgtg attcagagca cgaaagagaa ttatcgtaag
    11521 tgggaaaaca aataacgtaa aaataataag ttctatgatg ggaatattaa ccggcgaacg
    11581 attcagattg cagacgaaag aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa
    11641 gttgggataa cgtaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
    11701 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
    11761 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
    11821 gcgggttgag gtattgctgc atccccacgg ccagggtgta gctatttacg tcacgcagta
    11881 acagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaggatg aacaccaccg
    11941 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
    12001 catccagcgc cgccgcttct tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg
    12061 tccagacatg cagcgcaatc ccacccagat acgcgaaaat tacgccgcca tgagtattca
    12121 gaccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
    12181 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
    12241 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg
    12301 cggaaatccc ggcgaccttt accgagttcc acagccacag cagtaccggg aatggcggtg
    12361 gcgtaatgcg accatcagcc tgttcaacgc taaaacctaa cgccagtttc cagtgatccc
    12421 aggagatttg ctccgggatc aggctaccgg tcgcgaagtt tccctgacgc agcgagatag
    12481 cgacgaccat cagcagcggg aacataatcg ccgcgataaa aagtagcagt agcaggtgag
    12541 taataaacaa acgtgctttt tgcgatttcg gttggaccat tgccattttt gttatctccc
    12601 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcac ccaccagcag
    12661 gaagatcagc gtggcaattg ctgccgccag accgaagtcc tgacccccgc cgccttcaaa
    12721 agcgatacgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
    12781 aagacgatcc gggccgccgt tggttaacag ctgaatcagc acgaagttgt taaagttaaa
    12841 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
    12901 aaagaagttc tggaacggac ctgcgccatc cattgctgag gcttcataca aatcgtccgg
    12961 aatcgctttc agcaagccca tgcagaggat catcatgtac ggataaccca gccaggtatt
    13021 gacgataatc agcatcgtgc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
    13081 aaacagcgcg ctcaacatca tgttgatttc accgaagctc tggttaaaca atcctttgaa
    13141 aatcaagatt gaaatgaacg atggcaccgc gtagggcaga atcagcagga cgcgatagac
    13201 cgctttgccg cgcaacgctt cccactgcac cagacatgcc agaaccatgc cgaccgccac
    13261 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
    13321 tttctgaatg ccttcgtcgg taaagacgcg ggtaaagttt ttccagccgg tggtcacggt
    13381 gtaaccgggg cttagctttt catcacccca gttgccgtcg gcggtaatgg actggtaaaa
    13441 gccaatttgg ttattcggac gatatttcac gccgctctga ttattcgtca acgtgccgtc
    13501 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
    13561 tttgttgcca tccggcagaa tggcggtaat gtcactcagc gcctgacgat tctgggtaat
    13621 cacgcgcaga ttcgcgcgtt cgccttcggg ctgggcggtc gtttctttca gttgcagttt
    13681 ttgctcgccg ccaaatttaa acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct
    13741 gagcgccagt tgccactcat cgcccgccgg gtaaagacca aagttatagg ttttgcctgc
    13801 ttgccaggag cgatctaaca acacttcctg cgcacgttca aaagtcagct ggttagtgct
    13861 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
    13921 cattccagcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
    13981 atacagcccc gctgaactca atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc
    14041 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctagca ctgaccattt
    14101 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
    14161 tccaggacgg ataaggcttt cacgccttat ccgacaacaa ctgcctgatg cgacactgat
    14221 gcgtcttatc aggcatacat gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc
    14281 atccggcatt tcacagcatt acttggtgat acgagtctgc gcgtctttca gggcttcatc
    14341 gacagtctga cgaccgcagg cggcgttgat caccgcagta cgcacggcat accagaaagc
    14401 ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca tggtggcggc
    14461 aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgccactg cacccagcgg
    14521 tttgtcttta ttgaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
    14581 tttcgccagc tctttgttcg gactggcggc gttaataccg gcgctcagca cgccaacgaa
    14641 cggtttagat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat tcactttgct
    14701 ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc atcgctgttt cgcctttatt
    14761 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaataag
    14821 gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccca cgtctttaat
    14881 atcgtacttg ccgttttcat acttgaacgc ataacccccg tcagcagcaa tcagcggcca
    14941 ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
    15001 tttatccagc gccgggatct cttcccaggt ttttggtggg ttcggcagca gatctttgtt
    15061 ataaatcagc gataacgctt caacagcgat cgggtaagca atcagcttgc cgttgtaacg
    15121 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
    15181 agccaacagg ccagattgag cgtagccacc aaagcggtcg tgtgcccaga agataatgtc
    15241 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
    15301 aacggtgact ttaattccgg tatctttctc gaatttctta ccgacttcag cgagaccgtt
    15361 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
    15421 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
    15481 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
    15541 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc accgttgctt
    15601 tgtgtgatct ctgttacaga attggcggta atgtggagat gcgcacataa aatcgccacg
    15661 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagccg
    15721 tgttctcatc ctcccgcctc ctcccccata aaaaagccag ggggtggagg atttaagcca
    15781 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
    15841 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
    15901 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
    15961 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
    16021 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac acaccgccag cagaacgcgg
    16081 cgttggtatg gtgtttcagt cttacgcgct ctatccccac ctgtcagtag cagaaaacat
    16141 gtcatttggc ctgaaactgg ctggcgcaaa aaaagaggtg attaaccaac gcgttaacca
    16201 ggtggcggaa gtgctgcaac tggcgcattt gctggatcgc aaaccgaaag cgctctctgg
    16261 cggtcagcgt cagcgtgtgg cgattggccg tacgctggtg gccgagccaa gcgtattttt
    16321 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
    16381 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
    16441 agcgatgacg ctggccgaca aaatcgtggt gctggacgcc ggtcgcgtgg cgcaggttgg
    16501 gaaaccgctg gagctgtacc actatccggc agaccgtttt gtcgccggat ttatcggttc
    16561 gccaaagatg aacttcctgc cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt
    16621 ggagctgccg atgccaaatc gtcagcaagt ctggctgcca gttgaaagcc gtgatgtcca
    16681 ggttggagcc aatatgtcgc tgggtattcg cccggaacat ctactgccga gtgatatcgc
    16741 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat
    16801 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    16861 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    16921 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    16981 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    17041 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    17101 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    17161 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    17221 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    17281 aagagcttct atttcgacac taatgtggcc tattccgtcg cacaacaaaa tgactgggag
    17341 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaatctgat cgaatggctg
    17401 ccaggctcca ccatctgggc tggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    17461 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    17521 ttcggtaaac tctctctggc agtaacccgc tcctctgaag caggtggttc ttcctctttc
    17581 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
    17641 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    17701 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
    17761 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    17821 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggtgt tgcatatgtt
    17881 gacgaaaaat tttcctatga cattaacaac aacggtcaca tgctgcgtat cctcgaccac
    17941 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    18001 aactgggata acgacaacgg caccaagtgg tggaccgtcg gtattcgccc gatgtataag
    18061 tggacgccga tcatgagtac cctgatggaa atcggctacg acaacgttaa atctcaacgt
    18121 actggtgata ccaacaacca gtacaaaatc acacttgctc aacaatggca ggctggcgac
    18181 agcatctggt cgcgtccggc tattcgtgtt tttgccacct acgccaagtg ggatgagaaa
    18241 tggggttacg attacaccga caaagctaaa ggtactccga actacggcaa agccgttcca
    18301 gctaacttcg aaggcggcag cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc
    18361 cagatggaaa tctggtggta atagcaaaac ctgggccgga taaggtgctt gcgccgcatc
    18421 cggcatccaa cgcctgatgc gacgcttgcg cgtcttatca ggcctacaac ggctgtcaaa
    18481 tgtaggccgg ataaggcgtt tacgccgcat ccggcataaa aacaggttgt cattatctga
    18541 aaggggcgca agcccctctg attatcgggt ttagcgcgct attgcctggc taccgctgag
    18601 ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgcc ctctgtttat
    18661 cagcagggtt actggcaagc gcgcctggaa tcagccttgc cgatgttaac tacgtaccgc
    18721 aaaacaccag cgacgcgcca gccattccat ctgccgcgct gcaacaactc acctggacac
    18781 cagtcgatca atctaaaacc cagaccaccc aactggcgac cggcggccaa caactgaacg
    18841 ttcccggcat cagtggtccg gttgctgcgt acagcgtccc ggcaaacatt ggcgaactga
    18901 ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt atttgcgccg aacgtgctga
    18961 ttcttgatca gaacatgacc ccatcagcct tcttccccag cagttatttc acctaccagg
    19021 aaccaggcgt gatgagtgca gatcggctgg aaggcgttat gcgcctgaca ccggcgttgg
    19081 ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa agatctccag cagacgaccc
    19141 aactgctcga cccggctaaa gcctacgcca aaggcgtggg taactcgatc ccggatattc
    19201 ccgatccggt tgctcgtcat accagcgatg gcttactgaa actgaaagtc aaaacgaact
    19261 ccagctccag cgtgttggta ggaccactat ttggttcttc cgctccagct ccggttacgg
    19321 taggcaacac ggcggcacca gctgtggctg catccgctcc ggcaccggtg aagaaaagcg
    19381 agccgatgct caacgacacg gaaagttatt ttaataccgc gatcaaaaac gctgtcgcga
    19441 aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg ggatcgacat
    19501 ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg gtaattacgc cccacagtgc
    19561 tgattttgca acaactggtg cgtctcctgg cgcacctttt tttatgcttc cttcttggga
    19621 tatgagcgat tttttatagt aactcacttc ttcttcacta agaatgtcca ttatctcaat
    19681 gccttatcag agattctttt cctttcgccg gtagtgtctg gacattcagg ctacttttcc
    19741 aggttatttt atttctgtta tgcagaggtt ttatgataag tcatatccca aattctggcg
    19801 gcaataactc tttgatgaaa catgatgtgg tgcaaggaaa taatatagta gatcttgatt
    19861 tactacgtaa ttttaatggg gtgccaggtt taaatagaga taattttatt tatatcagca
    19921 atattttttt aaatataaaa caacggaacg aaaaaaatca tgcaataaat atgtttcgtg
    19981 aagtctcaat tagtaatgat actataagtg taaaattcta cagaaatgaa aaaatagaat
    20041 gcgcttgtaa ttttctgatg gataaagatg cgcaggggta tatcgacctg tctgatatga
    20101 atttaacaag ttgtcatttt aaaggtgacg ttatatcgaa ggtgtctttt ttatcatcaa
    20161 atctacaaca tgtaacattc gaatgtaaag aaattgggta ttgcaatttt actactgcaa
    20221 cagttgataa tgttatattt agatgtcgac gtttacacaa tgtgattttt atcaaagcga
    20281 gtggtgaatg tgtcgatttt agcaaaaata ttcttgatac agttgacttc tcgcggagcc
    20341 agcttactca tagtaatttt cgcgaatgtc agattagaaa ttcaaacttc gatcattgtt
    20401 atctttatgc ttcgcacttc accagagcag aatttctttc tgacaaagaa atatcattta
    20461 tcaaatcgaa tttgacagct gttatgtttg atcatgtgcg aatatcgaca gggaatttta
    20521 aagattgcat tacagaacga gtggaattaa ctattgatta ttcagatata tttgggaatg
    20581 aagatctcga tggttatatc aataacatta taaaaatgat tgatacattg ccagataatg
    20641 caatgatatt gaaatccgtt ctggcagtaa aactggttat gcaattaaaa attcttaata
    20701 ttgttaataa aaactttatt gagaatatga agaaaacatt tagccattgt ccttatataa
    20761 aagatccaat tatacgcagt tatatccatt ctggtgaaga taacaagttc gatgatttta
    20821 tgcgtcaaca tcgattcagc aaggtggatt tcgataccca acagatgatc cattttatta
    20881 acaggtttaa tatgaataaa gggctgattg ataaaaataa caattttttt atccaactta
    20941 tcgatcaggc cttacgatca acggatgata tgatcaaagc aaatgcctgg tatctttata
    21001 aagagtggat tcgtagtgat gatgtttcac ctctatttat agaaattgaa gataatttaa
    21061 gaacctttaa cacgaatgaa ttaacacgaa aggataatat ctttatcctg ttctcctctg
    21121 ccgatgatgg gccagttatg gtggtaagct cccagcgctt acatgatatg ttgaatccta
    21181 caaaagatac caattggaat tccacgtgta tctataaatc cagacataag atgttgccta
    21241 ttaatcttac tcaggaaaca ctttttagct ccaaatctca tggtaaatat gcgcttttcc
    21301 caatttttac tgcgagttgg cgagctactc gtataaagaa tataggtatt taagtaaagg
    21361 aaaacatcac cgttcctggc atcatgggcg gtgatgcccc tacggttgcc ctcgccagca
    21421 cgggcatcgg caaagcgtaa ggttcaacag cggtttacca cttcatgcga ttgttgcgtt
    21481 tttgttgcgc attagatcac ttaatttgct ttacatctcc cgtaaacact tttctgcgat
    21541 acaatgcctt tacgttatgt aacggagagt tcggcatgtc acaccccgcg ttaacgcaac
    21601 tgcgtgcgct gcgctatttt aaagagatcc ctgccctgga tccgcaactg ctcgactggc
    21661 tgttgctgga ggattccatg acaaaacgtt ttgaacagca gggaaaaacg gtaagcgtga
    21721 cgatgatccg cgaagggttt gtcgagcaga atgaaatccc cgaagaactg ccgctgctgc
    21781 cgaaagagtc tcgttactgg ttacgtgaaa ttttgttatg tgccgatggt gaaccgtggc
    21841 ttgccggtcg taccgtcgtt cctgtgtcaa cgttaagcgg gccggagctg gcgttacaaa
    21901 aattgggtaa aacgccgtta ggacgctatc tgttcacatc atcgacatta acccgggact
    21961 ttattgagat aggccgtgat gccggactgt gggggcgacg ttcccgcctg cgattaagcg
    22021 gtaaaccgct gttgctaaca gaactgtttt taccggcgtc accgttgtac taagaggaaa
    22081 aaaatatgga gtggagtctg acgcagaata agctgctggc gtttcatcgc ttaatgcgta
    22141 cggataagcc aattggcgcg ttactgctgc tctggccaac attatgggcg ttgtgggtgg
    22201 cgacaccggg cgttcctcag ctctggatcc tggcggtatt tgtcgcgggt gtctggctga
    22261 tgcgcgctgc tggatgtgtg gtgaatgatt atgctgaccg aaagtttgat ggtcatgtta
    22321 agcgcacggc gaaccgacca cttcccagcg gcgcggtaac agagaaagag gcgcgcgcac
    22381 tgtttgtagt gctggtactg atttcgtttt tactggtact gacgctgaat acgatgacca
    22441 ttctgttgtc gattgccgct ctggcgttgg cgtgggtgta cccgtttatg aagcggtata
    22501 cccatctccc gcaagtggtg ctgggcgcgg cgtttggctg gtcgattcca atggcttttg
    22561 ccgctgtgag tgagtcggtg ccattgagtt gctggttaat gttcctcgcc aatattctct
    22621 gggcggtggc ttacgacacg cagtatgcga tggttgaccg cgatgatgat gtgaagattg
    22681 gcattaaatc cacggcaatc ctgttcggcc aatacgataa attgattatt ggtattttgc
    22741 agattggcgt actggcactg atggcgatca tcggtgagtt aaatggctta ggctggggat
    22801 attactggtc aattgtggtg gctggcgcgc tgtttgttta tcaacaaaaa ctgattgcca
    22861 accgcgagcg tgaagcctgc tttaaagcat ttatgaataa taactatgtt ggtctggtac
    22921 tatttttagg gctggcaatg agttactggc atttctgatg atgtaaaaaa gccggatgat
    22981 catccggctt tcttctgggt tgcctgatgc gcggcgcttc tcaggcctac acaacacatc
    23041 gcaatttatt gaatttgcag attatggaag gccggataag gcgttttcgc cgcatccggc
    23101 aattctctct gattaccctt cgccctgcgt cgcactctca atcgtcaaac gcacgtctga
    23161 tgtaatcaac tccgccagca actgataaac cttcatcgtt tctgccggtt cggcgtcgcc
    23221 gctatcgctg atataccctt catcacgcag cgtcagcacc agagaactga acaccgcctt
    23281 gtcgaagaac tccggcgcgt tgatgccgtg cagcacggag agacgttgcg cgacggtgcg
    23341 gctctctttc tccagcgtac cgcggttgat cgacgggttg gcactcaaca accagaaggt
    23401 gatggcataa cgttgcagcg tttcgcgcgc gcctgcggcc agcagctgta gggtgcgaga
    23461 atgcgccggg ttgatatgca actcatcatc ttgcagggta atcagcccct gacgttgcat
    23521 ctcatttgcc agcgcatcaa taacgtccgg caactcgtcg cgatcccagc gcaggaacag
    23581 ctccgctttc agcattgggt aaagcacatt gacgtgctcc atcaatacgt cgcgggagat
    23641 gtggcgatgc tgggtgacaa ttgccgccat cagcgaaggc agcaccaaca tatgcgcaat
    23701 gttgttgcga tagtaggtca tcagcaccgc ttgctcgcgt ggcagaatga tgatgtcgcc
    23761 gatagtgtct ttctcgactt caaacttgtt catttgcagc gcgtgatcga taagctcgct
    23821 ggcgctggct gaaggaacgg tagagtccgt ggagtaaggt acgttgcgca tcagatccag
    23881 gtagcagttg agttgctcgg ttaactgctc gcgggtgagt gagcgctgac gtgatgccag
    23941 tagcgcagta cagcacaggt tcatggcgtt tgccgcgcct gcgttgttaa tgcgtaccat
    24001 cagatcggca gcaatattat tgaccgtcgg cgttaaccag gccggacgca ccgcttcgat
    24061 gggatcgata gattcacgcc agtctggtac gtgctggtta aggtaggtca tcaacggcat
    24121 tggttcaccg aagttgacgt aaccctgacc gagattacgc agcttgctta aaccgcgcag
    24181 catctgcggc aggctctctt tctctttcgt cgcgccgcgc agttctttgg cgtaagtacc
    24241 cacttccatg acgtgctcat aaccgatata gatcggaatc agcgtaatcg gacgcgtgcc
    24301 gccacgcagc atcgcctgaa tggtcatcga cagcgtaccg gttttcggat ccagcaaacg
    24361 ccccgtgcgg gaacgaccgc cttccacgaa gtactcgacg gaataaccac ggctgaacag
    24421 ttcgccgaga tactcacgga aaacggtgga ataaagttta ttgcctttaa aagtacggcg
    24481 aataaagaac gcccccagac ggcggaaaat cggcccggcc ggccagaagt tcaggttgat
    24541 cccggcggcg atatgcggcg gcaccagccc ctggtggtac agcacgtaag aaagtagcag
    24601 gtagtccatg tgactgcggt ggcaaggcac atacaccagc tcatgaccgt cgtgggccag
    24661 ctggcgaacg cgctcggcgt tatggacgtt gatgccctga taaagtcggt tccaggtgaa
    24721 gcccagaatg cggtcagtca ggcggatcat ctcgtaagag aaattcgccg caatctcttc
    24781 catcagcgca atcgcgttct gctgcgcttt ttcatgggag atttttttgc tgcgcgcttc
    24841 atcttctacc gctttggcaa tggcgcggga ggcgagcagc ttattaaaca gatcctgacg
    24901 agcaggcaga cgtgggccca cagcagccag acgttgacgg gcaaagtgca tacgcgccac
    24961 gcgcgccagt ttctgtgcga tagttttatc cgtgccgtgt tcatccgcca tacggcgcag
    25021 cgaaactgac ggcgagaaac gcacaaaact gtcgcgaccg agccacagta cggcgaaaaa
    25081 tttctgtacg ccgttaagca tacgcagcgg cgggtttact tcgccttttt cacgccccgg
    25141 cgcgcgacca aacatcaccg atactggcac catctgcaca tccagatttg ggttgctacg
    25201 gtgcaaatcg agatagtcgt ggaacagctt aatagactct tctttcggcg tgtaataggt
    25261 gaatacacgc ggcccgccgt gaatgaacac atagcgcggc agtagcgtgc cgtcgatttc
    25321 cagcggctct aacgggtcag gcaagtcatg agccagacac tgggcgcgta acgtcagcaa
    25381 atctgctttc gagttgtacg gtaaaacgta cataattgga cgagaagtat ccagccccag
    25441 ttccggggca ggatctgccg gaatagactt gctttttacc aggatgctta atggtaaatt
    25501 cagtaatttg tagtaaattc gtggccagcc ggacataaac gatgtaaagc ctctggttaa
    25561 taatgcaaat gcgcggcaag gatagcagaa agtcatggga aattctgtgg tatccgctca
    25621 tgtttcgcgc ggcgttacgc aaacccgaat catcggattt aacggtacac tgatattgac
    25681 gctcataatg taaaaaggtt ctttcaatgg ccaataatac cactggattc acccgaatta
    25741 tcaaagctgc tggctattcc tggaaaggtt tacgcgctgc atggatcaac gaagcggcat
    25801 tccgtcagga aggcgtagcg gtattgttgg cggtggtcat cgcctgctgg ctggatgtgg
    25861 acgcaattac ccgcgtgctg cttatcagct ccgtgatgct ggtgatgatt gtggaaattc
    25921 tcaatagcgc catcgaagca gtggttgacc gaattggctc tgaataccat gagctttccg
    25981 gacgcgctaa agatatggga tccgccgcgg tgctgattgc cattatcgtc gccgtgatta
    26041 cctggtgcat tctgttatgg tcgcattttg gataaccctt ccagatttcg ataaatctct
    26101 ggtttattgt gcagtttatg gttccaaaat cgccttttgc tgtatatact cacagcataa
    26161 ctgtatatac acccaggggg cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt
    26221 gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
    26281 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
    26341 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
    26401 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccgcttct ggcgcaacag
    26461 catattgaag gtcattatca ggtcgatcct tccttgttca agccgaatgc tgatttcctg
    26521 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
    26581 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgaa
    26641 gttaccgtta agcgcctgaa aaaacagggc aataaagtcg aactgttgcc agaaaatagc
    26701 gagtttaaac caattgtcgt tgaccttcgt cagcagagct tcaccattga agggctggcg
    26761 gttggggtta ttcgcaacgg cgactggctg taacatatct ctgagaccgc gatgccgcca
    26821 ggcgtcgcgg tttgtttttc atctctcttc atcaggcttg tctgcatggc attcctcact
    26881 tcatctgata aagcactctg gcatctcgcc ttacccatga ttttctccaa tatcaccgtt
    26941 ccgttgctgg gactggtcga tacggcggta attggtcatc ttgatagccc ggtttatttg
    27001 ggcggcgtgg cggttggtgc aacggcgacc agctttctct ttatgctgtt gctgttttta
    27061 cgcatgagca ccaccgggct gactgcgcag gcttatggtg ccaaaaatcc tcaggcatta
    27121 gcccgtgcgc tggtgcaacc gttgctgttg gcgttggggg ctggggcgtt aattgcgctg
    27181 ctgcgtacac cgattatcga tctggcgctg catattgttg gcggtagtga agcggtcctg
    27241 gaacaggcgc ggcgctttct tgaaatccgc tggttaagcg ctccggcgtc gctggcgaat
    27301 ctggtattac tcggttggtt actcggcgtg caatatgccc gtgcgccagt aattttgtta
    27361 gtggtcggca atatcctcaa cattgtgctg gatgtctggc tggtgatggg gctgcatatg
    27421 aacgtgcagg gcgcggcgct ggcgacggtt attgcggaat atgcaacatt gctgattggt
    27481 ctgctaatgg tgcgtaaaat cctcaaacta cgcggaattt ccggcgaaat gctgaaaact
    27541 gcctggcgag gaaacttccg tcgcttgctg gcgcttaacc gcgatatcat gctgcgttcg
    27601 ctgttgttgc aactctgttt cggcgcgatc accgtacttg gtgcgcgact ggggagtgac
    27661 attgtcgctg ttaacgcggt tctgatgacg ctactcacct ttaccgccta tgcgcttgat
    27721 ggttttgcct acgcggttga agcgcactcc ggtcaggcat acggtgcgcg cgacggtagc
    27781 cagttgctgg atgtatggcg ggcagcgtgc cgccagtcgg ggattgtagc gttactgttt
    27841 tcggtggttt atttgctggc aggggaacac atcattgcgt tgctgacgtc gttaacccag
    27901 attcagcagc tggctgaccg ctatcttatc tggcaggtga ttttgccgtt ggttggcgtc
    27961 tggtgttatc tgctggacgg catgtttata ggcgcaacgc gtgccgccga aatgcgtaac
    28021 agtatggcgg tggccgccgc aggttttgcg ctgacgctcc ttacgctgcc gtggctgggt
    28081 aatcatggtt tgtggctggc attaaccgtc tttctggcgt tgcgcgggct ttctctggcg
    28141 gctatctggc ggcgtcactg gcgcaacggt acctggtttg ccgcaacgtg acggttaaaa
    28201 attctgaata aattatccta agccaaattg ctgactacac ttaatctcac gttcagaaga
    28261 aaagtgaacg tactctcatt cacaacctaa cgatgaggtc ttgattatga ataaagatga
    28321 agccggcggt aactggaaac agtttaaagg taaagtgaaa gagcaatggg gcaaactgac
    28381 cgatgatgat atgacgatca ttgaaggtaa acgtgatcaa ctggtcggta aaatccagga
    28441 acgttatggt tatcagaaag atcaggcaga aaaagaggtc gtggattggg aaacccgcaa
    28501 tgaatatcgc tggtaattaa tccctcctgc ccgacgtgta caaggatgta cgccctctta
    28561 acgcggtttc tttttcacct gcacagagtg atcatgctgg cactgttcag gatgacgaca
    28621 cgcttccact tccacgcatg ccgcacagag cccatgtgct tcaatcacat tatgccgcag
    28681 ggcaaacccc atttttgccg ccagcgtatg cataatgtct tccacgcctt ctgcacactc
    28741 ttctttcacc gcgccgcagc gatcgcatat aaacatggct gacgtatgcg tgggctggtc
    28801 gaacagatga cagagcacat aactgttggt ggattccacc ttatgcacaa aaccttgctc
    28861 aagcagaaaa tccagcgcgc gataaaccgt tggcggcttg gcttgcggtt cagcttcgcg
    28921 cagtaaatca agcagatcat aagcgctgat agcgccatcc tgcagactca tcaggcgcaa
    28981 cacttccagg cgctgtgggg tcaggcgcac attacgctgc gcgcagattt tttcagcctg
    29041 cgctaataac tcctgcgttg tggtcttttc catcgggcac ctcaaagtgg gggttaagaa
    29101 tgtccttact ttaccatgtt ccaggaaaaa caccgagaac ccttattgtt gccgtaatgt
    29161 tgattttctg ttttgtaggt aaggtgttgt gtttccttgt cgtaccatta tcaacacgat
    29221 aataattaat aattcatttt taaataaatt gcttcaagtt aactcgctat ttatttaact
    29281 gggtttaaac gcatacctgt agttcagaaa ttatgagcga tttttgatag tgaccagatg
    29341 gcaaaagaat tataaggagg acacggtacg acaaaaaagg tagatgaatc atgttgaaga
    29401 taatacccgg tgcaactgga tactttaata aaacactcaa ttcaaatcaa ttcgataatg
    29461 aagatgcaat caaagataaa ttagataata gaggttcaat caaaggtaaa ttaaataata
    29521 tatatggtaa atctattgat tatgcagcgc tgcgtcatcg ggatataatt attgcaaaaa
    29581 tagatttgtt tattcaacga atcacgcata atttatggca tgcacgtaaa aaaatgtgtt
    29641 tttaattgag caaataaatg atctcaaaat gtgggtcaat aagtatattg acgattgcac
    29701 tgatgaggat ttaaacgatc gtgactttat tgcatcagtt gttgaccggg ctatttttca
    29761 tttcgcgatt aatagtatat gtaatcctgg ggataataaa gatgcgactc ccattgaaca
    29821 atgtactttt gatgtagaaa ctaagaatgg ccttccctcc acggttcagc tattttatga
    29881 ggaatctaag gataatgaac ctttagcgaa tatacatttt caagcaatag gttctggttt
    29941 tttaacgttt gttaatgcct gccaggaaca tgatgacaac agcttaaaat tatttgcttc
    30001 gctgttaatt tcactttcat attctagtgc ctacgcagat ttatcagaaa cagtgtatat
    30061 taatgaaaat aatgagagct acctgaaagc tcagtttgaa aaattatctc aacgtgatat
    30121 gaagaagtac ctgggagaga tgaagcgtct ggctgatggg ggagaaatga attttgatgg
    30181 ctatctggat aagatggcac atctggtgaa tgaaggaacg ctcgatcctg atattttaag
    30241 caaaatgaga gatgctgcac cacaattaat tagcttcgcg aagtcgtttg acccaacctc
    30301 aaaggaagag attaaaatac ttacagacac ttctaaatta atttatgatt tgttcggggt
    30361 taaatcggag aaataatatg tgaagttctt cgatagtatg gaaggcatta tataaaagga
    30421 cccaatattt attgggttct tttttctcta tcaatgctat tagcagggag atatatcacc
    30481 agagtttaat gtgtgatttt ttatttatcg tcgaacctgg attgtttatc attggcctta
    30541 acaaagttaa cggctaataa gattatttcc atcacttcgt gagagcttca tgccttgaga
    30601 ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt atagtacccc
    30661 gctattgagc ctcctgaata gtgatgctga ataacataac cccatgatat atcgataaaa
    30721 taatctctac atttgaaaat gcacggtaat tctgaaatgc aaaaaatcaa ccaaaccagc
    30781 gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc accaatgctc
    30841 gataggatgt accgttgttg aaaaacaagt agttatacac tctgtgggag cctattggga
    30901 actcggcgtt ttcatttcaa ggtgtaatcc atacgcggct taagaatgag atataatgcg
    30961 acttttagtg ttccgcttga gaggccatga tgcttaccct ggacgagata ggtcaatcag
    31021 tacgtaacaa tatccagttg attattgatc atgtcggctt acctcttgct gttggtccgc
    31081 tcagtgatga tgattacaag attctgtgtg gtggctatgg tgagcttgaa tgggactatg
    31141 cgttaagtac ctatggcaac tccagagaaa agtatgagtt ctgcataaaa cttgttcagc
    31201 aaggtcgggt tcagggaata ccatcaggag cagcaatttg tgtttatggg gttgaagaaa
    31261 acatctttcg tatccatatg atcgaaaggt tttctagaga agatgaatct cacccattga
    31321 aagggcgcat ggttttactc actcttatga gtgcttttat attttgtaaa gctgttgaat
    31381 gtaaagttgt ccacattgta gagccagtac cagaactggt gcagtattac gagtcttttg
    31441 gtttccgcat ggaacagtgc ggttatgtga tgtcggcagt cattgatgag ctgcaggata
    31501 tctttcttaa atttgctcag taggtataga cgagaagggt ctacaaattg taggataccc
    31561 gtccagattt ccttaaaggt acatctatgg cagtcgtttt gtgcttaaac tactaagaaa
    31621 cgatgtcacc aatcgacatg atcgattggc ataagttagc gaaacaagct agctttaaag
    31681 aaagggttag agacgccttt actgtctcgg gagttttcta tgaaagatca aaaagcaacc
    31741 aagccacagg ttaagttcga cacaatgaaa gcattcgcag gtatgggtgc tgctgttgaa
    31801 gttctgatga aggctgctcc taatgcgttc actcacgcta ctgtctctgg taaagagcag
    31861 cagggtaagc ttcgtcgtcg caaagcagca tgatcatagc tggtgctttt tgaaaacccg
    31921 ccttcaggcg ggttttttct ttagtgatgt tctttgccct tctgtttgac tgttctgacc
    31981 tgttcccact cgatacgtcc ttcttctcgt cttttgtcta tgtattccgc aagatcctga
    32041 atattgatgc aacgttttgc tttttgtgat gtgccgatgc gatatgttgg aatgggcaac
    32101 ttacaagcgt ttgcttttgc ttctgccgtg gctggactca taccaaagta cttttggcta
    32161 actgctgaga gttcaatgtt tggggtattg aattcagcca tcagtaaaaa caaggtgttc
    32221 ataattttct ccatcaaaac cggctgcacc cgggaaaatc ataattctgt gctggtggca
    32281 ggaattaatt tctgccagat agctgaaaca tatttggcct gatgacgagc atcagccagg
    32341 gcgttgtgcc gttcgccatc gaaaggcatg tccatttttg ggtcgaatcc gatggaacgc
    32401 ccaagcgtaa cgatcgtgcg tacatcgtga tcattccagt acgcccatgg gcagatttgt
    32461 cctgctcgtt cataagctcc gcgtaaaatt acgttgtcga aggtggcccc gttaccccaa
    32521 acttttaaat atttcgtatt gtctgcgtgc cgattaatga aatggctcag ttcagagagt
    32581 gcatcgctga tcgacaaagt atcatcaata cagattgcag ctcgtgcttc agggctttgt
    32641 ttcaaccacc acaggatggt atcgccgtca ggtgtggccc cttgctccat agcactgtcc
    32701 aggctgacaa ccgtatagaa ttcttgtccg atgtctccgg tttctggagt gaagaacacc
    32761 gcgccaatgg aaacgatcgg tgcatcctta tttttcccca tcgtctcaag gtcgatcatt
    32821 aagttattca ttacttcacc tcctgcgttt ctttgctgct gtgaattcgc cagttaccga
    32881 cgccttccca ttcaaactgg cggttactaa ttcgcgtcca gcccctgccg aaaagcaaat
    32941 ccagatacca gtattttaaa gttctgataa ttgctcttac agttggtttg catccagaag
    33001 tctttgatgc gcaaataaag caccgagtta tgctcatggc ttctacaaaa ggccagataa
    33061 accaaaccca gatgcaaaga gccacgaaca acatgagcgc gatgttagcc actaagccag
    33121 accagtacag ataattgctc attggttgcc tccaccttgg ctctgaagca tggcagcgcg
    33181 gcaggcgttc cagccatcaa catacccagc catgatatat cgattaaaac ccacctcttt
    33241 ctgtgcgcgt gccagattca tttcctccgg cacgtgtact ggctgagcta tatacagcgg
    33301 ctgaacatac caaccctttg ataaccaact gtcagcaacg tttttactcc gtgttattgc
    33361 cggaatacct aagccattgt ctgaatgcag ccatgccacc ggctcctctt ctagcgatgc
    33421 cagagcaatt tcataagcac ggcgctcaat atcgtctcga acctctaggc tgctgattcg
    33481 ttctttgatt tctttaatca gttctttatt ggtaaatgtg gtcattatgc tccagcctcc
    33541 ggcgcttttg gcattactgc ccagtgagtg atattgaagt tttcaaggtc cccgacctga
    33601 aatgtccact gccattctcc ggtttctttt tgtccccagg tgtaccagag agaacgccag
    33661 ccaatcagcc agccttctcc gttagcatca aataacagaa cactttcatt tgctggtggc
    33721 agttcagctg acactggtat tattttgttt tccagtgccg cacatttagc ttcaagcgcg
    33781 tcgaatttac gtactaggta ctcagcattt gtttcgttca ctttcagatc tcgcggtaca
    33841 catttcccgc gaagaaaccc ttccatttcg aaaacattca tgcgcatttg cgtaactccg
    33901 ataactcgtt aaaacgttcc ataaacatcc cgtaggcatg gctaggtgcc agtggaatca
    33961 cgttgaacat ctctgttgcc gggatgcctt ccagtacagg ccagaaagag ccatcatcaa
    34021 gcccgagatc gcggcgttcg gttgccagca tgataagatc ggcatatttc acgggcgtac
    34081 tcataactgg gggtaacccg tatttctcac ggattacggc gtcaattttt tcttccatcc
    34141 gtttatagtc aggaagaagg cgtttcagtg gagcgggaat atcctggcaa tacgcttctg
    34201 ttgcatcatg cattaacgct tcaaaagcaa attcctgcgg caccagctgg ctgcaaagca
    34261 ccgcatgttg ggcgacactg tagaagtgag aaagatgacc ggcaaagcgg cagatatttg
    34321 aaagggaaac cgcgatatcg ttaatcacga tgtcgtcttt atttatcttg tcataataaa
    34381 aatgcttccc ggaaaaagtt ttaataaatg acattttgtt ctccacgtat atgcgctgca
    34441 ccgcgctgag tttgggtaaa aggaagccct caccatccgg tgattattga gttaattacg
    34501 tttccataaa tgcccccgca ggggcatttg cagtaatgaa atcaggcggt gaaagtacca
    34561 ataaaggttt ctacgttgct gtctttgaat ttctcaacaa gcagatcacg aaattcgtta
    34621 gccatatctt cctgcaccgc ttccagctga ataatgcgca gaaccagtac aggacgatcg
    34681 ccagtgataa tgctgaggcg taatttaaac ggacgttctt tcagaccttc aaacggaacg
    34741 catttaaatt caaatgccac tggcataatg tctttggtct tcgcttcgac agattccatc
    34801 agggagcgtt tgccgctgaa gtcattatct tcaaaatcag cggtctggtt tgcttcaatc
    34861 gtgattttac ggactgccgc agccgctttt gttgcctgaa tagcgtcacc attagcatca
    34921 aagcccacaa ggtagtcggc ccagtcttca atccattctg ccagtgactt ctgggagtta
    34981 cgctcgccgt taacagacaa cagggcagag aacggtgctg tctttttcag tttgagagtg
    35041 gcggtgttat ctgcgtgacc tggttcatca atagtaccca ggttaagtac actgacggca
    35101 cgcatattat cagcatcgat aaagcagcgg gtgccttcat ctgcaagatc tttagaataa
    35161 cgggtaaagt catcgatgct ggcagtggaa agcgcaccac ggaaacggaa gcgatttaaa
    35221 ttaaattttt ccagatcatg aatgcggaaa ttctcaggca atgccacagc atcggcacca
    35281 atcttactga taatttcatt aacaccctga gcagaaataa gggcatggat ttgattaatt
    35341 gcggttgcgt ctaagttctg agacataata agtcctcact atataaagat attcagtgat
    35401 gagataaata atcagttaat taagaacgat attaatgacc tgctgcgcgt agttttccgt
    35461 caggttcacc ggcaagagtc agtaattgtc cctggtcttc ctgcagaata gtcaggcgac
    35521 caccgcgatt gacatacatc ggcgtttcgg tggtgtcttc ttcggaaatt ttcccgcggt
    35581 tagtcgggcg aacatatgag agtttgtgtt tgattttcac acggttctca tcaaatggtt
    35641 cgatttccag gttgagtgag accttacctt tggttttcgt gttcatcaca cctgaagcga
    35701 cttcactgag aactgcgccg attttggttt caaatacgcc gccgtccagc tccccgataa
    35761 atgcctgcac atcagtactg cgttcgctag ccattttgct gctcctcatc atatcgaccc
    35821 tgcaaggtcg gttggtttct ccacaaaaca gagaagaaca cctgcggtgg cagccgcccg
    35881 gatggattgg gttatgagcc cgtcgtccgg tgatgctctt ctctgttttg taaaaagagc
    35941 ggtaccagcc ggaagcaagt gtacaaactg gtaccgccaa agcagtggct gttgtggtga
    36001 ccggtgctga tctccggctt gcggttattt cagactctca cgggcgttta attgccccgc
    36061 cgaacagctc ttttccgcaa tagctgcaat gtctttcgcg catcagcctg cgcattcacc
    36121 acaacgctga gagcacttag ccagttacgg caccacactt tgtcgcggct ccataaatgc
    36181 cctcatcgtt gccccctggt ctcttcccag gcgtcaaacc gaatcgccac gctggttagg
    36241 cgtcttatca gcatcatcat tgacttgcac attccggcta cctggtttgt ttgcccgagc
    36301 agggagtgga tagtcccctt taacgtcccc agaccgctaa cgacgcatgt gccatacgcc
    36361 gtgatacaac aatatgttcg gtattagtta ccaaagagac gattactctt atggttgaat
    36421 gtaataccca tgttgaatat ttttcttgct tactatttgt tttatctatg tggtgaactg
    36481 gtttttgttt acacaagaaa aagttttctt aagtaagttt acattgtttt gtcttgctat
    36541 ttttttaaat tcaagaacaa taaattgccg ctatcagtaa ttattcttta tgaaatttta
    36601 taaataactc cctgcttttg agttctgtac gcagtgtttt tagttgtcga ggacataatg
    36661 gataatcaaa atacacctgt ttttgatgta tgtggttggt actgtgtgcc cgttaaagat
    36721 cataattcag ttatagttca actgtcttgt aaacaaccac catttgaatc tgataaaatt
    36781 atcgaaagtc caatatattc ttttcccata aaagaaatac caaatctcat aaatacatta
    36841 caaaacattt atgaagaaaa taattgatta taaatccagt tcaaaagtga agcgtccatt
    36901 tgggcgcttt tttatttacg aatcattccg gtcttcgtat gccccggacg gctacttcgt
    36961 gggcgtcctg cctgttcgat atcttatgta ggtacattat gtatctgtag ggtacattgt
    37021 caagtataaa aaaacctgcc gaagcaggtt catagacatt gatcaggctt taattttgta
    37081 tcttcttggt tttcctgaga aaataactgt accaatgata gagcaattac cgtttatctt
    37141 aatgtaaggc tcaggccagt tggggtttaa tgctttgaga taacgctgtg tcccgtcttc
    37201 tatcaacctt ttgaaggtgg tttcacctgt atcgtgcatc aatgcaataa cgtcatcgcc
    37261 gtggcaggct ggtacttctg gatcgacaaa aatcatgtct cccggacggt actcatcaat
    37321 cattgaatca ccaatcaccc tcaatatata agtcatttcg ccacagggta cagggcaggg
    37381 atacgtttct gttgtgctca aatcaacctc agaatagcca acttctttcc atgctccggc
    37441 ctgtacccat gatatgacag ggactaacgt tattttttta ttagtgattg aaacatcagg
    37501 tttttttgtg atgttcgttg tctggtgttc ttgatcaagc catccgacag gcaggtcgaa
    37561 acatttttcg atgtgccgtg ccatgctgtc accgatattt ttagtagcgc catcccccat
    37621 aaacctgctg gtctgggtag gctcgcgatc aatcatggtg gcaaaggatg aatttccgcc
    37681 aacaccatct ctcagttttc tggcgttaga ccgccggatg tcatggattg ttttcataac
    37741 gaaattaaaa cctttgtacc gataaggtac aagtatcttg aaggttcatc tcaatcatgt
    37801 aatatgtata ctggaggtac atgttgtatg aaagcgtatt gggactcttt aaccaaagaa
    37861 cagcagggcg agttggccgg aaaagttggc tcaacacctg gctacttacg gctggttttc
    37921 aatggttata aaaaagccag ttttgtgctg gctaaaaaac ttgagcaatg cacgtcaggt
    37981 gcaattacga aatctgactt aagaccggat atctatccga aagattaaca gaacaccttc
    38041 aatttttaac cacagaacga tgaggctaac cgtgggtaag catcactgga aagtagaaaa
    38101 acagcctgag tggtacgtga aagctgtcag aaaaactatc gcggcgttgc cgggaggtta
    38161 cgctgaagct gctgagtggc tggatgtaac agagaacgct ttattcaacc gccttcgtgc
    38221 agatggcgat cagattttcc cgctgggatg ggcaatgatt ttacagcgcg cggctggcac
    38281 tcactacatt gcggatgctg tcgcacagtc tgctggtggg gtgtttgtat cgcttcctga
    38341 acttgaggaa gtagagaacg ccgatataaa ccagcgcctg ctggaagtca tcgaacagat
    38401 cgggagttac tcaaagcaga ttcgttcggc aatcgaagat ggggtagtgg agccacacga
    38461 gcagacagca attaatgatg agttgtatct gtcaatttcg aagctccagg agcatgcagc
    38521 actggtctac aaaatcttct gcgctccaga aaagagtgac gcccgcgagt gtgcagctcc
    38581 gggcgtcgtg gcgttttgtg tctgtggaga aactaacgca tgaacagttt aacggcaaat
    38641 aaccgtttgt cgcaacagct ggtggtcagt gtcgctgaac acctgttgtt acggcatgaa
    38701 tgcagattac caaatcacct ggctgtaagt aaccacagag aactttacct gactgtgggg
    38761 ggcgagttgt gcaggaactt aaccgctggt ttcgtgacgg aagagggctt tatgtccatg
    38821 ttattcgttg ggagccagaa acacagcgcg ttatctatct tcgcaaagac tacccgcatg
    38881 agtgctttag tcctttgtgg aaattcaggc gtgattttgt tgagtgtgaa ggaccaccag
    38941 cacattgatt ctgccattcc gggacgttac actgttcagg caccttataa aacgggtgcc
    39001 gggattggcg tcctgaaatt gattactgag cataaccgcg ctcatgcggt tttttcgtgt
    39061 catgagcatt gctacgccca aattatggtg gggcgtacag ggccgacttc ggtcgggccg
    39121 ggttcggtag tctccggtaa cgccaaccct gtacgtctca ccacctctgt gattggcgtc
    39181 ccatgtggtg agttttctaa aaaactgact accggggctg tcaccatgac tactctccca
    39241 accctctctc aacctgaaat tgccatcgtt gatggtcagg ctgttacatc ttctttggct
    39301 gttgctgact tcttctccaa acgtcatgac gatgtactga aaaagatccg cacgcttgaa
    39361 tgctcagcat cattcactgc ccgcaatttt tcggtgagtg attacactga ttgcacaggt
    39421 cgcaaacttc cttgctacca aataacccgc gacggctttg cgtttcttgc tatgggtttc
    39481 acgggtaaac gtgctgccca gttcaaagag gcatacatca atgcctttaa ccagatggag
    39541 aaacagcttt caaagccctc tgtaccgagc gacgttgcac ataacgccag cgttctctat
    39601 tcctacattt catcaattca tcaggtctgg ttgcagcagc tttatcccat gctggaaaaa
    39661 gctgaatcac cgctggctgt aagtctgtat gaccgaatta acgatgcggc atttcttgcc
    39721 cgtcttattc attcgtcgct gaactcttca gaggtaaggg ggcgcaaatg atccggaata
    39781 ttttcaaacg ttttaccaat cagactttcc gttgtcctcg tccgggtcag tggtacacca
    39841 cgcctgcagg gcatgttcta cgtgttagcc tagttgaccg tgaatgccag aaggtgattt
    39901 gtgaaccgct gggccgtaat taccgcgtca gtatgccgct tatagccttt cgctccggaa
    39961 aaaacatgaa gcatctcgga ggtgcagcat gagtatggag ctgatggtta aagcgatgaa
    40021 aattcgagtg ggtaatccat tacgaaaact ggttctgatc aagctggctg ataatgccag
    40081 cgatcagggt gagtgctggc ccagctacca gcatattgct gaccagtgcg agattagcaa
    40141 acgttctgtg atgaatcata ttgcggccct ttgtgagtcc gggctggtaa aaaaagtcac
    40201 ccggaaaggt gaaaaaggta actcaagtaa tatctatctc cttcatctgg atggtgcagg
    40261 agattcacta gggggtagtg caaataattc actatctggt gcagcaaatt caccaggtag
    40321 tgcaggagtt gcaccagggg gtagtgcagg agattcaccc agaaccagtc actcttttga
    40381 accagtcaaa gaaccagtca atgaaccaat agctgttggt gcatctgctg atgagtctgt
    40441 gcgagttcgt tcaaaccgac cggaatactc tccggagttt gagcaggcat ggctggcata
    40501 tcccaaacgt gctggtggca attcaaaatc tgcagccttc aaagcctgga aagcccgttt
    40561 gaatgagggg gtaaatcccg aaaccatgct ggaaggtgtg aaacgctatg cgggctgggt
    40621 atctgcgatg ggtaacagcg gcacacaatt tgtgaaacag gctgtcacgt tctttggtcc
    40681 ggatcgtcat ttcgaagaat cctgggaagt tcctgcggta tctgcagcca gacgcgagga
    40741 cccgtacttc aaagccagtt acgacaacgt ggactacagc cagatcccgg caggattcag
    40801 ggggtgatca tgagtctgtt aaatgacgtt cagaaattca ttgaagccca tccggggtgt
    40861 acttccgcag acattgcgga tgcttttgca gattactcac ggcagcgcgt tctgcagtca
    40921 gcaagcaagt tacgtcagag tgggcgtgtg gctcaccgtt gtgaaggaga tacacgcaga
    40981 catttcccgc gcctgactga gagagcgcag gagctggaac cacaaccagt tcgtgaaacc
    41041 agacctgtgc gtaatttctg tgtcggcact aacgatcccc gggtgatttt atgcctgacc
    41101 cgccaggctg aagaactgga gtcgaggggg ttataccgtc gtgctgcaac ggtgtggatg
    41161 gcggcattcc gtgaaagcca ctcccagcca gaacgaaaca attttctggc gcgtcgtgag
    41221 cagtgcttac agaaaagcag caagcgcgca gcatcgggtg aagagtggta tctgtcaggg
    41281 aattacgtgg gggcttaatg agtaataaat attgtcaggc actggtggaa ctgcggaaca
    41341 aaccagccca tgaactgaag gaagtgggcg atcagtggcg cacgccggac aacattttct
    41401 ggggaattaa cactctgttt ggcccgtttg ttctggatct gtttactgac ggtgataacg
    41461 ccaaatgtgc cgcgtattac acggcggaag ataacgcgct ggcgcatgac tggtcagaac
    41521 gtcttgcgga gcttaaaggt gctgcctttg gtaatccccc atacagccgc gccagtcagc
    41581 atgaggggca atacatcacc ggcatgcgtt acatcatgaa acatgccagt gccatgcgtg
    41641 ataagggcgg gcgctatgtt ttcctgatca aagcggccac cagcgaagtg tggtggccgg
    41701 aagatgcaga tcatattgct tttattcgcg ggcgtattgg ttttgaactg cctgcctggt
    41761 ttataccgaa ggacgagaag caggtgccga caggcgcttt cttcgctggt gctattgctg
    41821 ttttcgacaa gacctggaag ggaccggcaa tcagctacat cgggcgcgat gaacttgagg
    41881 catgtggtga ggcgtttctg gcgcaggttc gccagcaggc ggaaaaactg gtcagggaga
    41941 tggcggcatg acgacgttaa ctcaatgcca gcagcaggtg ctggatatgc tgatttctta
    42001 ccagaaagaa cgtggcttcc cgccaaccaa tcaggaggtg gcaaccatgc tgggataccg
    42061 ttcagtgaat gcagcggtgg aacatcttcg cgcactggag aaaaaaggcg tcatcacgat
    42121 aaagcgtggc gtggcccggg gtatcactct tcataccgcg gtgaaggacg acgacagcga
    42181 ggcggtcggg attatccgct cactgcttgc cggtgaggaa aacgcaaggc tgcgtgcaac
    42241 tcactggtta catgagagag gcctgaaagt atgaagctga tcctgccttt cccgcccagc
    42301 gtgaacacgt actggcgaca ccccaacaaa ggggcatttg cttgtaagag cctgataagc
    42361 gcggcggggc gaaaatttca gagcgcggcg tgtgcagcaa tagttgagca gttacgtcgt
    42421 ctgccaaaac caacgtcggc acctgcttca gtggagatcg tgttgtttcc tcctgataac
    42481 aggatccgcg atctggacaa ctataacaag gcactgtttg acgccctgac acacgcgggt
    42541 gtgtgggaag acgacagcca ggtgaaaaga atgctggttg agtggggacc ggttatcccg
    42601 ggagggaagg tcgagatcac tatcagtaag tacgaaaaag tgagttgcaa attagcaacc
    42661 cggtaacgga attgagcaac accctaaatt tgggtattac ctcgttaaag atactgtatt
    42721 tatgaacagt atatccttga taactattaa aaatcgcagt aagttcatcc tgcatcaacg
    42781 aaaagggagt gcagtcccga aatttgtgga gaaaccaatg aatcagttgc ttgtaattga
    42841 tggcgtttct gtgcgccagt acttcgaatc taactactgt cttaacgacc ttcagaaagc
    42901 tgctcttctt gccgctggtg agagtcgttc ctcccgttcg ctggaagttc acgagtttat
    42961 gcgtcgtcct gaaacgaagg ctcttgtgga attactggaa gaagaaacta cgggagattc
    43021 ccgtagtatt cctgtcatca ccattcaggg gcgcaatggt gggacgtatg tttgtaaaga
    43081 gctggtctat gcatatgcaa tgtggatcag cccggcattc agcttaaaag tgatacgaac
    43141 ttttgatgcg cttcataatt catcaccaga agaaaccaca tccgacaaaa ttaaatccgg
    43201 ggtcattctg cttgaatcag cagcaaagac tctaaatctg tcaaactcct cgaaacttgg
    43261 tgcataccag aaattatcaa aggtagctgg tcttcctgaa cttatgccga tctatgccat
    43321 tgatgcacct gctgatgcgc cagatggttc aagccgccct acgctgtcgc tgagtgcact
    43381 gctgaagcag tatggtatcc gcctgacggc taatcaggca tatcaccaga tggcgaagct
    43441 ggggatcgtc gagcagcgcg aacgatacag ccgtaccgcg attaacaaca tcaaaaaatt
    43501 ctggtcgctg acggcgaaag gctgtatgtt cggcaagaac atcaccagtc ccgcaaatcc
    43561 gcgcgagacg cagccgcatt tcttcgaatc ccgattccct gagctgttaa agttgctcga
    43621 taccgttcat tgaggtgacc gtgagagcac tactgacccc tgaaatagcc ccgcgtatgg
    43681 ggatcgtatt gttcagacca ggttcagagc tgatgcccct gtttatgcag gggcgtgtac
    43741 tgctggagcc tgagccggaa cgttattcat ctttcgccag tggtgccgtt cccgcagcat
    43801 cacaaccgct ggcggatgat cctgctgttc gggccgtgtt ccgcaatgag gcagtgatcc
    43861 gtcgtgctgg tggcgtggaa tgtcttgaaa gctggttact tcgtgaaaag ggctgtcagt
    43921 ggcctcattc caactggcac agcgagaaca tgaccacaat gcgacacgct ccgggcgcaa
    43981 tccgtctgtg ctggcactgc gataaccagc tgcgcgatca gttcacggaa cggctggaat
    44041 caatggcaac ggataactgt gcccgctggg tgttgtctgt tgtgcgtcgg gatctcggtt
    44101 ttgatgatag tcacgttgtg acaatgccgg aactgtgctg gtggctgatt cgtaatgatc
    44161 tggcggatgc cttaccggaa agcgcagccc gtaaggcact gagattaccg aagcctgttg
    44221 tgccgtctgt cacccgggaa agtgaccttg tgccttcggt tcctgccacc agcatcatcc
    44281 aggataaagc gaaaaaggtg ctggcgctga aagtggatcc ggaatcgccg gagtctttta
    44341 tgttacgccc aaaacgtcgc cgctgggtta acgaaaagta cacgcgctgg gttaagacgc
    44401 agccgtgtgc atgttgtgga aagcctgctg atgatcccca tcacctgata ggccacggtc
    44461 agggtggaat gggtacaaaa gcgcatgacc tcttcgtgct gcctttgtgc agaaagcatc
    44521 acaacgagct gcatgcggat accgtggcat ttgaagagaa gtatggctcc cagctggagc
    44581 tgatatttcg ttttatcgat cgcgcgctgg caactggcgt gctggcgtaa gtggagaacg
    44641 agcatgaacc ttgaggcctt accaaaatat tactccccaa aatctccaaa attgagcgat
    44701 gacgcactgg cgacaggctc gggtggttta acgattacgg atgtgatggc tgcgcagggg
    44761 atggtgcagt caaaagcacc gcttgggttt gctttattcc tggcaaaagt tggtgttcag
    44821 gatcctcagt ttgcgattga aggtctgctc aattacgcga tggcactgga taacccgaca
    44881 ttgaacaaat tgagtgaaga aacccggtta cagatcatcc cttaccttgt gaattttgcc
    44941 tttgctgatt attccaggtc tgcggcaagt aaggctcgct gtgagcattg tgcaggtacg
    45001 ggatttcata atgtattgcg cgaagtggtg aaacactcca gaagcgggga gtctgttatc
    45061 aaggaagagt gggtgaagga attatgtcag cattgtcatg gtaagggaga agtcagcaca
    45121 gcgtgcagag ggtgtaaggg taaaggtatt gtcctggatg aaaaaaggac ccggcttcat
    45181 ggcacgcctg tttataagat ttgtgggcgt tgcaatggaa accggtttag ccgtttacca
    45241 accacactgg cgcggcatca tgtccagaag ctgataccag atctgacgga ttatcagtgg
    45301 tacaaaggat atgcagatgt cattgataaa ctggttacaa agtgctggca ggaagaagca
    45361 tatgctgaga cacaattgag aaaagtgaca agataaatga ttttcaccga agatggcgac
    45421 atgatgcttg cattttcaaa aaatatggat aaaatttacc aaacgatggg ctttgtatgt
    45481 ctaccgttga taagatttaa gaacccgcca ctgagcgggt ttttttgtgc ccgaaaataa
    45541 gcgtggtgct ttaagcttgc tgcaggctgt gagtacaggt cgttctgttt gcagtttttc
    45601 gataagaaca ataaatcaga tggtaattga aatgaaagct ggttgttaat ttcgcgttgc
    45661 gttttctttg ttttctgaat aatgaaacat actaggacat ttatgttcat taattttgtt
    45721 cttattgagt atctctgagt tattcgtatc attctgccgt gccatcgcaa tgatgcgatg
    45781 cgggcccttt agctcagtgg tcagagcgag cgactcataa tcgccaggtc gctggttcaa
    45841 gtccagcaag ggccaccagc cgtcactagc tcatcgggac agagcatcaa ccttctaagt
    45901 tgattgtgcg gggttcaagt ccccggtgac ggtccagtgc cgacttagct cagcaggcag
    45961 agcaactgac ttgtaatcag taggtcacca gttcgattcc ggtagtcggc accatatgcg
    46021 ggcatcgtat aatggctatt acctcagcct tccaagctga tgatgcgggt tcgattcccg
    46081 ttgcccgctc cagtggacat gagttataaa acttggcagt gctgatgttt tcgtggagag
    46141 cgaaaattcc cttatccatc tccactctgt gacagacgca ggtcgcagac tcagcactgt
    46201 tttttttaca gcattcagat ggtgcgttgt ctgttttggt tcggagagac agggtacgta
    46261 gcgcgcagga cctggactga tgacgcgcca tctcaatgtc gttaatataa gaggtgcccc
    46321 atcacctcaa aaataatata tgtcgtctta tgcgttcaac cgccgtatat ggcggttttt
    46381 tttattccct caattttctg tttatagagg ttattcgcaa tgcgtaatgt gtcgtttgac
    46441 gttcgctatt cctttcatct tgaaacgatg atggcgaccc tgtaccgaag gctggaatat
    46501 ctgctttatt ttattctgat gctggctggt gggcttgttc tggcagatgt gggtaatacc
    46561 gcgttacagg ggctggtggt catcattgtt gctttcacct cgctcacact tcgtcccgca
    46621 agaaatgcgc tgctgtgtga acggcaggct gcaagatacc gtgagctcat gcatgatttt
    46681 gagtttcatc cggatgaaac agagcttccc gatatggatg aaattgagct gcatgacaac
    46741 accgcaattg gcctgctgag gaatgctgca tacaagagaa cgctgatagt gctcgggtat
    46801 tcacaggagg caaatgccat tcatctcaca atgatggaga aaatcgcagc atggtttgcc
    46861 ggtgatctgc cggaagagtg tgtgcccgct ggtgcagtgg ataacgaaat ctgaaaatgc
    46921 ctgttttcag ttgagagata cgctccggaa gaccaaactg attaacgcaa aggtaataca
    46981 tttattgttt tgaaaatccg atcagcacgt ttgcttttct gccagctccc ccagagctgg
    47041 ctttttttga ggtctgctcc ggcaggcttt tttatttccg cgccacgccc ggcgcatatc
    47101 aaaaaccaca gagccttaca gaaatgagct tcggagaaac accgtaataa gcggcggctt
    47161 ctctgtgggc ggtgtttctg ggcgacgagg ctcatttttg taaggagagc gtgatgaact
    47221 atccagacat tgttaacggc attgattttc gtgatctggt tatggttgcg ggcgattccg
    47281 tcctgaccac gagtgttaaa gtggcagaat tttttggcaa gagccacaag aacgtattaa
    47341 gaaagatcag acaaatcatt tctgaatgcc ctgatgattt tgcccggctc aattttgagc
    47401 ccactgattt tattgataaa aatggcgaca tccagccaat gtttaacatg acgaaggacg
    47461 ggtacatgct ggtcgtcatg gggtttaccg gcaaggctgc aatgaaaatg aaagttgcgt
    47521 acatccaggc tttcaactgg atggcggagc ttattcggca gggagtggca aatcttgagg
    47581 ctgaacgcaa tgccgtcatg ctggaataca tgaaagagaa ggatgttgcc agtatgtcgg
    47641 ggcgtttgct taaccgctgg ggaagagtga aaaagccaca gcttcaggca aggatcgaac
    47701 aacttgagca gcgagggcag atatccctgc ccggtatcga cagcgtatta acacactgag
    47761 gtctgctacg gcaggccttt tttgtgtcgg cacacagcag cgcaatagtc actgttagtt
    47821 tgttcccggg agggataaat gaaaattaat tcatgttttt ttcatgaaaa aggttttttt
    47881 gtggcgtgcg atcgccagga tgacttcttc ctcagtttcg ggcctgatat tggcgtgggg
    47941 cgtttctcat tcattcgccc cgcgcgggag catggcgtta ccgcagggct ttttcaatta
    48001 acggaagtgc tgccggaatg ttcattaccc cctgagtcag taattgcagg gtcaaatgtt
    48061 ttatggcgtc tgacggaagc tctcgaagtt ttgaaatcag tcccttcttc tgatcttcag
    48121 gaatattggc aatccggatt atgtcctcta tagcagtcag ggtgctgtga tgaaatttta
    48181 tggtctgaac tttcagtatg gcgctcagac caccatcatt tcgcagaaaa tcaatgccat
    48241 tgattgtgat tgctggcatc cagacatggc tgtatccgca tgagtcaagt cgtaatccac
    48301 tggttataag accatgacct tccagatata aaagattagc aatcgctgtt ttgttgtcag
    48361 ggaataccga taacaggcgc tttaactgct cattgtttct tattttgtcg gggaatatgt
    48421 cgttgagaga cgataagaac tcgagttgcg tttgacgatc gaacttgtcc atgatgagtg
    48481 cttgcttttg atagtgttaa ttcatgaagt ttaactccgc caaagttatt tattcaaccg
    48541 cccttcaggg cgggcagaag ccttcacatc gacactgagg tcgccatccg gcggcctttt
    48601 ttgtacccga atgaaaacct tcatctgtac tgccagcggt ccttcgctgt ctgctgagga
    48661 ctgccggttt attgcggact gcggtctgcc tgtcatcgcc gttaacagca cctggaaacg
    48721 ggttcctttt gccacgcacg tgtttgcctg tgacttctcc tggtggaagg cctgcgtccg
    48781 tcacatcacc attccggctc agcgctggac tgacagccgg gctgctcaca agcgcttcgg
    48841 cattaacttc tttcctgaag agcgcggctc tccggttaac tccggcagac gggccattga
    48901 gtttgcgctc tggctgggcg cggagcgggt gattctgctg ggatatgact gctccgtctc
    48961 acgcggaaca cactggcacg gtgaccatcc ggaggggctt aagaacccga cggagaacag
    49021 cacgcgtctg tggcggagcg aatttgaaaa acacgcgccc gtactgaatg cacgggcgga
    49081 aatcatcaac tgttcggtgg aaacagccct gacctgcttt ccccggagac ggctcaggga
    49141 ggttcttgat gccactgtca aaaagtcgcc ccgtgtatgt taagggaatg cacgggattg
    49201 gcgacaacat taaccagcga atgttcattg atgcgctttc gcgtgccgga tacagggtgg
    49261 agctacagac acccctgccg cagctttacg cggacctgcc gggggtgacc ttcattccct
    49321 cggagaccac cctgcgtacc cagcagaaaa atgagcagcg ggcctacagg agcctgccgt
    49381 acatcgcccg cccctcagcg gatgcacagc gtatccgcat catgcatgac cggcagacga
    49441 aggggcgggg ggaaagcgtc gttatgacaa tgacacgaca gttcgggatt gcgccagctc
    49501 cggcgtctct tccgtcgttc aggcgttcat cactggtggt gaccgacaaa ccggtggcgg
    49561 tgattcgtcc gacaacggtg cgtaatgagt ggaagaccac ctcccgtgga ccgctgaatg
    49621 agtacatcga catggcctcg cggatgctcg ctgcgcgcgg ctatcactgt gtgagtgtgg
    49681 cagaccttaa cgagtctgat gcctgcgaaa agcgtcgcga atggattgac ggcatggtgc
    49741 cgtttgcgca tcagcgattt catcacggtg agctgtcact tgaagagctg ctgttactga
    49801 ctgagagcgc ggctgtggtg gtctgcgggg actgtctgct gctgcacgca gcgatagcgt
    49861 acggtacccc gctgctggcc attcttggtg gcatgggcgg tgcctgtcgt cctgagatta
    49921 ttgctgaccc gtcccactat gacctgtcga aggtgtgctt cattaagccg gacaatttct
    49981 gtctgtgtca tgagatgacg cacgactgcg acaagcacat cagcggagtg gaaggcaaaa
    50041 tcgcggcgtt tctggatggc cccgtattca ggggaggctg atatgcctga caaatcaccg
    50101 ggattctgga tgtccctgct ggcctggttc cgggcggagc cggatgtgtc tgttgctgct
    50161 gcaaccgggc tgatggccct gctgcgttcc tgtcagcagg gaaagtcact gagggcgatg
    50221 ttgctggact catgcatctg cgcctttctg gcgttttatc tgcgtgatgc gctggggctg
    50281 tgcggtgtgg accaggactg ggccatgatt ctgggcgtgg tgctggggta tctggggaca
    50341 gagttcatcc gcaccctgct gattcgctgg gcggagcgtc gcgccgggat tacccgacac
    50401 tgaaggagtt ctcattatga acaaaaatgt gaaggccttt ctggatgcgc tggcgtggac
    50461 agaaggcacc agtaccagcc gttacacgaa aaatgacggt tacgacatca tcgttgacgg
    50521 catcgactcg ccgcacacct tcacggacta cagcacacat ccggctgttc tggtcacggt
    50581 taaccgtcgc gggctgaaat ccacggcggc agggcgttac cagctgctgt cccgttactg
    50641 gccgcattac cgcgacctgc tgaaactgcc ggacttctcg ccggcctcac aggatgcggt
    50701 ggccgtgcag atgataaagg agcagggggc atatgcggat gtggtggcgg ggcgtactga
    50761 gattgccgtg cagaagtgcc ggaacatctg ggccagcctg ccgggggccg gatacggtca
    50821 gcgtgaggtg acgatggcga tgttcgtggc gcagtaccgg cgctgtggcg gggtgacggc
    50881 atgagtgtga taccgcaatg gcgttactgg tggcgtttct actcgacgca tgccctgctt
    50941 atcgcctctg ccgtgccgct ggcgatgaat gaggcggaat catatctcgg gcagtcgctg
    51001 ccgttctggc tgaagctgct ggttgccctg gtcattctgg tcagtggtgt gactggtcgt
    51061 ctgattgtgc aggtgaagga gggggagaat gtggacactg ctcagcgcac tgattagtgc
    51121 cattgccgcc ttcttcggtg ccagaaaggc tgataccgca cagagcgaaa aacagctcat
    51181 caaccgcacg tcccgtaccg gggctgacat cacccgaaaa acacagagga aaacagaacg
    51241 tgaaattcgt gaagcgactg atatcagcgc tgctgattcc gggcgcgtgc gtgcttctgg
    51301 cagcgtgtca gaacgcaccg gaatcattaa cgacgcaatc cgacgggtta acgacgaagg
    51361 ccaccgttaa ctgtggtgag gacacgcctt tccacacgct gccgtcttat ccggagtgca
    51421 gtgaatgtga ccagactgac gaccctgctg ttctgcgcga ttacatcgct gctcagcagg
    51481 cgtgggccgt tgatgtcatc ggcgtccaca cctggaacag catattacgc gacaacaccg
    51541 ccggatgtct cagacaactg cgggacaacg gcatcatcaa ctgaacctga atcagaggaa
    51601 aaaaacatga gtgtgtcact gagtaccctg acaacgattg tctccggcgt gtcagcgata
    51661 gcgtcggggg tggtcagtgt ttacgaaaac ggcgcgaagc tgctggacat catgaagtcc
    51721 gccattgtga aggtggaaga ggcttacgaa gcgctgaagg cgtcaggggc caccaaaaag
    51781 gcgctggtgc ttgccaccct gaaagcggcg gcagagtcgc tgggggagga atgggacaac
    51841 atctgcgcct atttctcggc gctgattgac tcggtggtgg acctgtacaa cacggcggtg
    51901 gcggcaatat ccggtacgac gtatgaagac gccaccggcg aagaggttac cgcctgataa
    51961 cgccctttca tcctgaaggg gcttttttta cgaggagaaa aaatgccaaa aatcacatta
    52021 acggttgaag acaccatccg tattgtactg aacaacgatg atgtgctttc tctggtgaag
    52081 cagaaatatc tggaatccac gccgccggaa tttgacctga gcgaactgga ggcgctggtc
    52141 agtgacacgt cagatgatga cgaaccagag gctgacgaat cagagactga tacggacagt
    52201 ctggcactgt ctgaaacgac ggtcagcgga aaggcgggcg acacactcac cgttcaggtc
    52261 accgccgcag gcgacagcga cgtgcaggtg aatacctcag acctgagcgt tgcgacagca
    52321 gaatatgcgg acggcgtgct taccatcact ctggtggcag aaggcacggc aaccatcgcg
    52381 ctgacgaaag gtgagtcctc tgcggaggtg gcagtcaccg tgacggcaga ctgataaaac
    52441 aaaaccccgg ctgcgggaac agtccggggt ttttagtttt ctgaccttgg gtaaggcaag
    52501 ggagaacatg aggaagtata aacgaattct gttgaggttg actatgaaaa acggccttga
    52561 attgaaagcg cctgtaactg atgacatcag cagagcggtg gcttttgcca ttaagtgggt
    52621 ggcggttggc atagccgtgt ctccgatgct gtatgggctg gcaaaattga ttgttgctct
    52681 gaaatcgtga gtggggatgg gtgttatgag gagcatggca actgatgata aaagcagaaa
    52741 ccactccgca gggtgagacg gaggggtatc cgtcggcggt gactgtcacg ggtaataccc
    52801 gtctggttca gacagacaca tcggggcgta cctggcgtgt catgggagaa gacggtacca
    52861 gtttatagcg gggcgtgaca tcgtcgccgt gcccctgcat taacgatgaa cgccgtggta
    52921 gcgggcggga gtgtgcaggg gtaatcagaa acgatgcaac accaggtgag atacaacctt
    52981 tccgaccggc tttccggtgc cggggtgggc gaaaccggaa tctgtttaac tcctgatggt
    53041 gcgaagctat gaataacaaa gaaaacgtga aatttaccag tgagttaatt tgcggtgcaa
    53101 tgactcatca gatagcaggc atcactgatc gcgtgagaag ccgggcattc tgggaatgtc
    53161 tccttcgtga agcgccggaa gaattactgc ctctcctgat ttcccgggca attattcgcg
    53221 gacgggtatt tatggccgcc ggtgatatcc ggtttcctgt tgaacctgaa ccgaaggcag
    53281 acatgtttgc tgatttcagg gaagagctgg aaaaagcaga gaagcgcgaa ctgatgtgta
    53341 ttgccctgaa gagccagtta tcaggagagg tggatgaagt ccgtatcact gagttctgga
    53401 aagcattcct gagactgtcg tcccgtgagt tgctggcaga ggtgctgact gaagtgattc
    53461 atgaatgcag caaaaaggtg agatgtggct gaccttaacg accttcacag acagcttcag
    53521 gggatgcaga agcaggtgcg ttttgccact gcgcgcgcca tcacctcaac cgtgaggaaa
    53581 attgaagcgg cagagaagga aaaccttcgc cggaagctgg ataacccgac gccgttcact
    53641 gtgaactcgg tgcgctcaaa aggggccaca cgggacaatc tgaccgggcg ggtgttcatc
    53701 atggataccg ccgtgcctta ccttgaaccc tttgaggtgg gcgggctgca ttatctcggt
    53761 gaggggcaga aagctgtact gaacccgaaa aatatccggc tgaacaaata cggcaacctg
    53821 ccaaaggcaa aactgcaaca gctgaaggcc agaccggatg ttttcatcgg taaggtgatg
    53881 aacaacgatg gtgaggccgt tggtggcgtc tggcagcgta agaaagcgaa aaaagtggca
    53941 aaaaccagga agcggagaaa acgctccccc aatggcacac gcgctccccg caaaaaaaac
    54001 cgtccaccaa agctgttgat acgttttggt gatgcactgc cggtcacgcc ggtactgggt
    54061 tatcaggaac tggcccatga catggcatcg cgtctcatga agacggagct gaatgtggcg
    54121 cttgaacagg ctttaaagac ggcgaagtga tgcagacagt acagcaggtg ggtaacgggt
    54181 ccttcctgag cgggttattg cacgggcatt gcgcgagcgc ggtgtttcgc cagctataaa
    54241 ttttcaattt tgcgtcccaa tgtcccattg ttaatttttt gtttcattcc ttgctgtgtc
    54301 tggcttaagt ggtgttttga cctgggtttt gtgtcccaaa aaatgggaca ttttgatgtc
    54361 ccaatgtccc aatgtcccaa tgtcccaatg tcccaatgtc ccatttcatg tcccatcaat
    54421 gggacattcg ttttcgatgt cccggttttc ggaggtgatt cagtgagctt aatgacagtc
    54481 gggcagtacg ccgaacatgc cggggtgaac cggaaaacaa tttcacgatg gatcaaggcc
    54541 ggtacgtttg ttgtcatgga cggaaaaaaa attgacgtgg aggcgacaga taaaaatctg
    54601 aagcgttttc gtgacagcgc cgatccgcgt gcggcaaacg gaaaaaaaaa cagagtcgcc
    54661 agaaaagcta gggaatcgca gtccggcacg gtggaagggt tcgagcaacg ggcaagagat
    54721 gtgtttgatt cgctcacctc cggggatgtt gttatccgtc cactggaaga gtcccgcgca
    54781 atgaaagaac acttttttgc ggagatggcg cgtctggaat atgaggataa ggacggaaag
    54841 ctgtttccgc tgaaactcgt tacggaacag gtcgctgctg aatacacgcg agtgaggacg
    54901 caccttgttg cccttgcgcc tgagcatggt ccccgtctgc gggcgctggc gggaatgacg
    54961 gacgatcagg gcttcacggc agcgctgcag gagttggtat acgaggcact ggatgaactt
    55021 gcatttgaga aaagggaaat cgaagaagac ccttcttaag aaagcgctga tccagttgca
    55081 acgacatctt gcatcaccgc agccgatgtc cttaagccag tgggccaaca ccttcgctgt
    55141 actgtcccgt gagaccagtg cccagacggg gaaattccgc tcctatccct atcaggacgg
    55201 catgatggat gccatcacag acccgtcggt cacgtatgtg tcagtgatga aatcagcccg
    55261 tgtgggatac acgaaaattc ttgaccatgt ggtgggctat tacctggcac atgatccgtc
    55321 ccccatcctg gtggttcagc cgagggtgga ggacgccgaa gactacagta aaaccgaaat
    55381 tgccccgatg ctgcgcgaca cgccggtgct ggcagccatc agcggcgacc cgaaggcaaa
    55441 aaacagcaac cagaccattc ttcgcaaaac cttttccaac ggcgcaaacc tcacgctggt
    55501 cggggccaac tcccccggcg ggtttcgccg tatcacatgc cgcatcatcc tttttgatga
    55561 ggtggacggg tatccggcag ggggcgcagg atccgaaggg gatcagattg cgctgggaac
    55621 aaaacgttcc gaaaccttct ggaaccgcaa gattgtactc ggctccacgc caaccgtgaa
    55681 aggcgtgagc cgtattgaaa aagcctggct ggaaagcgat cagcgccgct acttcgtgcc
    55741 ctgtcctcac tgcggtgaat atcaggtgct ggagtggggc agcaaaagca ccccttacgg
    55801 catcaaatgg gaaaaggaca gcgaaggtaa cgggctgccg gagaccgctt actacctgtg
    55861 ccgccaccac ggctgtgtga ttgtgcacag tgaactgccc gggatgctgg caaaaggcga
    55921 atggcgcgcc ggtgcaccat tcaggggaca tgccggtttt catatctgga cgggctacag
    55981 cccgcactca aatgcctcct ggccgaatct ggtcgccgag tggctgaggg tgaaggacga
    56041 tccgctgatg cgccagacat tcatcaatac cacgctgggc gagccttacg aggatgcagg
    56101 cgagtttgcg atgagtgagc agcggctgat tgcccgcgtt gaggtctggg gggcggaggt
    56161 gccggagggc gtgctcctgc tcaccgccgg ggtggataca caggatgacc gctttgaaat
    56221 caccgttctc ggctggggca tgaacgaaga gtgctgggtg attgcgcatg acgtgatttt
    56281 tggtgacctg gaaacagaag agccgtggga gcgccttgac gcgtatctga aacaggtctg
    56341 gcggcgggct gacggtttcg ggctgacgct ctcggcggtc tgtcatgact cgggcggtca
    56401 tcacaccaac aaagtgtacg agttctcgaa ggcgcgtatc gggcgtcgta tctgggcgac
    56461 aaaaggtgag tccgccactg gcggcaaacg taaccctgtc tggccgacgc gtgtcgtttc
    56521 gtcccgcaac cggaaatcct tccgccccgt cattctgggg gtgaactccg cgaaggacga
    56581 catcaggcac cggctgcata ttgagccgga tccggcaggc gcgccgaccg caggctgtat
    56641 ccattttccg tcgtttctgg atctgcacta tttcagtcag ctgctctctg agcgtctggt
    56701 gcgtaaggag agcggcgggc aggtgtaccg tgtctgggag ttgtcttccg ggcgggcgaa
    56761 tgaggcgctg gactgcatgg tgtacggtta cgcggccctg aaggggctgc tgcatcacgg
    56821 cctgaaactg aaccgtctgg ctgaacggac ggcgcaggat ctgtcgttta tggcgccgcc
    56881 tcccgcgcag ccggaagaaa aaatcagttt aagtatgccg ggcgcaaaag caccggagac
    56941 accccgtaag aaaataccgc tgcatatgag gcttgcagga gtgagacgat gatgaatacc
    57001 cccggcgtgt ttgccggaat gtcaaaagaa caactgaagg cggcactgaa tgaagctcag
    57061 gcggcctata ttgagctgct ttcagggcgg cgtggcgtga gtttctccta tgcacagggg
    57121 gacggcacgc gcaccgtgac ttattcacag gccagcagtg cggatttgct ggcgctgatc
    57181 gcgacgctcc agcgggcgct ggggataagg acgagaaggt ccctgagcgt gcgttactga
    57241 ggtgagcgat gatagtcgat aaaaatggca gaccttttcc gcaacagacg gaaaaaaaac
    57301 gcgcactgaa tgacagcggg cggatcccct atgactctgc cggtttctcg cacggttcag
    57361 tggcgggctg gaacccggta ctgtggtcac cggacaatga agtgaatatc tggcgcaacc
    57421 gtatggttgc cagaatgcgc gaccttgtcc gcaatgacgg ctgggcgaat ggcagtatca
    57481 cccgcctgct ggacaatgcc atgggggtgg tttttcgccc gaggatgaag cctgactacc
    57541 ggatgctggc agagatgacc ggcaaccggg catttgatgc agactgggcg gatgagtacg
    57601 ggcgttgtgt ggaagcgcac tggcgcaact gggccggtga tgccgggtgt tactgcgacc
    57661 ttgagcgcag acagaccctg ccacagcttt tccggctggc attccgccac aagatggtcg
    57721 acggcgatgc gctggcggtc atccactggc gaccggacag aatagccccc ggtcgcgggc
    57781 gctacggtac ggtggtgcag gtgattgacc ctgaccggct gagtaacccg aacgatgcgt
    57841 ttgaccagcc gcatatccgt ggcggtgtgg aaattgacgc ggacggggtg ccggtggcct
    57901 atcacatccg gtccgcacac atgggggact ggtggagcgg cagtgacacg atgcactggg
    57961 aacgcgtgcc gcgtgaaacg tcgtggggaa ggccggtggt ggtgcatgac tttgatcatg
    58021 accgtgctgg ccagcaccgt ggtgtcggaa ttctcaatcc cgtggttcag cgtctgaaaa
    58081 tgctcatcaa gtacgaccag gtggagctgg aagccgccat catcaatgcc atgttcggtt
    58141 ttttcatcac gtcgccctat gacccgaaac tgacagaaga tctgatgagt gacgcggagg
    58201 tgattaatgg ctaccaggat gcgcgcatga agtatcacga tactaaccgg atttcgatgt
    58261 cgggcgtgcg tattcccatc acgttccccg gcgaagagcc aaaagccgtg agcgccgccc
    58321 gtccggtcag taacttcaag gagttcgagg gcacagtgct caggaatatt gccgctgcac
    58381 tggggctgtc cacccagcag gtgacacagg actggtcgga cgtgaactac agctctgccc
    58441 gtgcggcact gcttgaggcg tggaaaacac tgacccgccg tcgtgatgag tttgcggtcg
    58501 gctttgccca gccggtactg acggcctttc tggaagagct gcacgaagaa gaggatttgc
    58561 cgctgcctgc cagtgcaccg gattttctgg cggcgcgggc cgcttacacc cgtgcccagt
    58621 ggatggggcc ggggcgcggc tgggtggatc cggtcgcgga gaaaaaaggg gccattctgg
    58681 cgatggaggc cggcatgtcc acgctggaaa tcgaagtggc cgagaacgtg ggggaagatt
    58741 tcgaggatat tctcgaccag cgggcgcgtg aagagcgggc ctttaaggaa agaggcctga
    58801 caccgccttc ctggtttcag gcagagcagt ttgcccccac accgacgggg agcgccccgg
    58861 cagagccgaa ggagcctgat gtttcatgaa tcaacttgca ctgttaagcc agcgcctgct
    58921 gaacacgccg ctggcccttc atccgcgaaa agcggaaatc attgttacgg cactggcaga
    58981 acgcctcggg atcacccgga taaaaacggg ggcctggtat gacgatgatg aagacgattt
    59041 ctgccgtccc gcccgtgaaa gcgggtacga cattctggag gggatcgcca tcattcccgt
    59101 gcacggcacc ctggtacaga aactggggac actgcggcct tattccggca tgacagggta
    59161 tgacggcatc cggcgaaatt ttctgacggc gcttaacgac cctgatgtga agggcatctg
    59221 tcttgatatc gattcacccg gcggagaagt ggccgggtgt tttgatctgg tggacacgat
    59281 ttttgcctgt cgcgggcaga aaccggtgca cgccattctc tcggagtcgg cgtactcggc
    59341 ggcctatgcc cttgccagcg ccgccgaccg gatcctggtc ccccgcacgg gtggcgtggg
    59401 gtccgttggt gtcatctatg tgcactgtga catgtcgcgc cagatgaagg atgaagggct
    59461 gaacgtgacc atcatcacct gtggcagccg gaaggcggag accagtccgc tgcgtccgct
    59521 gagtgaggcg gcacaggcag cacttcaggc ggacattgac gccgcaggta cgctttttat
    59581 cgaaaccgtt gcccgcaacc gtggcatcag cgcggatgcg gtgcgtgcac ttgaggcccg
    59641 gacgttacgg gctgacgaag gggtgcaggc aggtcttgcc gacgatgtga tgtcaccgga
    59701 tgaggccttt tcttttctgt taaaggagac agcaaacaat gggtaaattt tctttcatgc
    59761 acctggtagg catgggcggt cgttcgcgaa tggatgacga tccggaggac aaacgcgacg
    59821 acgagaatgc aggggatgat gaggaccgtc gcgaaagccg taaggcgcgc tcccgtgcgg
    59881 atgacgacga taacgacgac ggggagaatg cgggggatga cgaggagcgc cgcgaaagcc
    59941 gtaaggcgcg ctcccgtgcg gatgatgacg ataacgacga cgatgaaaac gcagaagacg
    60001 acgatgacga agaaaagatc cgcgccagtg aacgtcatcg ctgcgcagcc atcttcgcca
    60061 cgccgtatgc ggcaaaaaat acggcccttg ccgcagagct ggccttcaac acccgcatga
    60121 gtgtgaaaca ggccagcgcc gtgatgaaag cggcggtggc ggtatcctca ggccagaaag
    60181 ccagactggc ggagcggatg cagcatgtgc cacagccggg aaccggacgt gatgtccgcc
    60241 ctgcaccgac ggaggcgcac gcgatggcac agcacgccat gaaactgtac aacgaggcaa
    60301 tgggaggtaa agcgcaatga gcacatacgg aaacaacccg tcggtaccgg gttactggtc
    60361 ggacacatac caaccggacc agctgttgtg cggcccgctt caggtggtca cgaaaaccgt
    60421 gaccatcacc ggtggtgagg tataccagcg cggtacggtg ctggggcgca tcacggaaag
    60481 cggcgcgtac accctctgca ctcaggggac gggcgtgacg gacggcagcg aaacgccggt
    60541 ggcgattctt gccgacctgg cggatgcctc gtccggtgat gtgctagccg gtgtctacct
    60601 gatgggtgag ttcaacgcaa accgggtgat ttttgatgaa agctgggaca ttgacgatct
    60661 gagcgtggcg ctggaaaaag agaaaatctt tctgcgcaac ccggtcacgg tgccctgatc
    60721 cccactgaga gcctgacagc cgcatgatgc ggcttttttt atggaatttt ttcatgagcg
    60781 aaaaagactt taatctgctg tacgacaccg cgtttctggc gcaggtggtg ccgaacctgt
    60841 ttgtggccca gaactggctg ctggacagtt tcttcccgaa tatcgtgctg agcgatacgg
    60901 agtccgtggc cattgacgtg gaagtgggcg cacgccgtat gtcgccgttc tgctcccctc
    60961 tggtggaagg accaatggtt gaggccctgc cgtatcagac caacatcttc aaaccggcct
    61021 acatcaagga caagcgcgtt cccgatttgc ttcgtccggt gcgccgtatg atcggcgaac
    61081 gcctgggcgg gcgtgagtac acgccagcag agcgtgcaat gctgaacctc cagtacgaga
    61141 tggccgacca gatcaacatg ctgaaccgtc gtcttgaatg gatggcggca caggcgctcc
    61201 agtacgcgaa ggtgacgata gccggtgagg gctacccgac cacggaagtg gattttcgtc
    61261 gtgacagtga cctgacggtg acgctgagcg gtgatgatgt ctggccttct gaggaaagca
    61321 gcacgatccc gaccacctgc ctggaggcgt gggcgatgct gatgctgaaa aaatccggtg
    61381 cgtacccgac cgaggtgatt ttcacgccgt cggcctggaa ggcgttcatg aacgacagct
    61441 tcattcgtga gaacgccatc aacatgcccg ccctgaaccc gaccagcaac gtggtgaacc
    61501 cggggacgca gatcaacacc ggtgcggtgt acaagggcaa atggggtaac ttcaacctct
    61561 ggctgtataa cgactggttc atcgatccgg atgacggaac cgaaaaaccg atgctggatg
    61621 acggcaacgt cattctgacc ggagcagccc tgatgggaac ccgcgcgttc gcctgcatca
    61681 tggatccggc gtttaattac ggccagatgg cctatgcccc gaaaatgtgg gatcagcagg
    61741 atccggcaca gcgtttcctg atgatgcagt cggcaccgat tgtcatccca tcccgtgtca
    61801 atgcgtgcct gtgcgcgtcg gtggtgtgag atggcgtccg gcaaaaaggg cgcgccggtt
    61861 gactatgttg tgctgcgcgg ttgtgtgcag cacaacggac aacgggtggc gcagggccat
    61921 gtgattaccc tgagcgaaag tgaggcgcag cgtcttctgc gtctgggggt gattgtttca
    61981 ctggcggaga tgaagggtga tgaccggacc ggttgactgg gacaaaaacc tgctctcccc
    62041 gttgttcggg gtgtttggtg aggaatgcga acaccgcccg cgtggcggtg aggcgtaccg
    62101 gctgacgggg gtttttgacc gggcgtacac gcagcagctt gtcggcgagg acggaggcac
    62161 ggaatcaaac tccacgctgc ccgtgctggg tgtgcgggat gcggaatgcc gcgtgaagcc
    62221 gaaacagggc gatatttttc tcatcgtgcg gacggggaag ctgtatatcg tgcgggatgt
    62281 gcagccggac agtcacggcg gcacgcgact tgaactgaac agggtgaagg aatgaatact
    62341 gcagaagcca gaaaaattgt cacccgggca ctgagtgatg ccaccggggc tggctggaaa
    62401 gtgtattccc cgcgcaccct gccggtaatg cccgatcagt atccgctagt cattgtgtcc
    62461 gtgcagtctg aacacaaggt gtcacagggg cggcatgtgc cgcagtacac gaccacgacg
    62521 acgttgcgca tcgacggtcg cgtactggcg tatgacagcg gggacgaaac ggagagcgca
    62581 gccggtgttg cgtgggaaga ggcggaggcg atgaaggagg cgatagagcg ggccgtcatc
    62641 ggtaatccgg ctgtccgcat gaagtttcag caaatcgctg atatccgggc gcatatcggt
    62701 gtggactccg acggtgaagc acacaccggt atcgtggtgc tggagctgga tctggatctg
    62761 gagtattacc aggggccgga ggatttcttc ccgtctgaga ttgtgccact gcgagaggtg
    62821 aacgtgcgcg gcgtgcatcc gtcattccgc cttcattttg atttacctgc cggtgatgaa
    62881 accggcagcg aaacctgaca ggagaaattc catgtttgtg aaacctgtac cggggcgcag
    62941 ggtgcgctac cccggcgacc cgtcacgcct gctgcctgat gagggggcgg aggtgccgga
    63001 tcgtgatttg ttctggcgac gccgcctgaa gcagggggat gtggtgctgg ctgacaaagc
    63061 caccacggca gccagcggca aagtgaccgt gtccacggca gcaacaggca gcacggcgaa
    63121 agggggtgat gcatgacggt ctcgttcagc aatatcccgt caaatcttcg tgtgccactg
    63181 ttctacgtcg aagtggacaa ctcgatggcg aacagcgcta cggagacaca gcgcacactg
    63241 ctgattggtc agatgacggc agaggggacc gccacggcag ggacggcata ccgctgttcg
    63301 tcggcctcca cggcagccgg actgtgtggt gaaggctcaa tgctgcatac catgctgaca
    63361 gcctacctta aaaacgacag ctacggagag acctggctgc tgccgctggc ggatgatgac
    63421 agcagcatga cgacggccac gggcagcata tctgttgaca gtgtgccgac ggcatccggc
    63481 gtcatttacc tgtacatcgc agggacccgt gttcgcctga cggtgaagtc aacgtacacg
    63541 cagtcggaga tagccagtct gctggcagcc agaattaatg caacctctgc gctgccggtg
    63601 atcgcatcgg tggccagcga cggcacgacc attgaactca ccgccagaaa tgccggcgag
    63661 gtggggaaca ccattgacat ccggctgaat tattccgggt catccggcgg cgagtccacc
    63721 ccggacggcc tgacgctgac catcacggcg atgagcggtg gcgaaggtgc gccggacctt
    63781 gcggatgcgc tcgccagtct gggtgaccgc acgtttgatt tcattgtcct tgcttatccg
    63841 gacaccacat cgctcaacga tatgaaggat tttctgtcgg atgacgaagg gcgctgggcg
    63901 tgggacaaac aaatctacgg tcatgccttc acggcggcga gcggcagcta cggcgagctg
    63961 gcggacaagg gggaaagccg caacgaccgg cacatgacca tctggggcgt gtatgacggt
    64021 ccgaacactt cgtatgatta tgctgccgcg atggtgggag cgctggcgca gtcggtccgt
    64081 aacgatccgg cgagacctac gcagacgctg tcggtgtccg gtgtgctggc tccaccgctg
    64141 gcaagtcgtt tcaccctgac ggagcggaac acgcttttgt acagcggcat ctcaaccttc
    64201 acggtgtcag acgacgatac ggtgacgctt gagaacacca tcaccacgta ccagaccaac
    64261 agctacggtg cggcggacga cagttacctg cagatcgaga cgatgtacac actgatgtat
    64321 gtgatgcgct ccatgaagac gcaggtgacg tcgaagatgg ggcggatgaa gctggcggac
    64381 gatggcgcga atattccggc agggagtgcg attgtcaccc cgtcgatcat ccgggccgaa
    64441 ctgattgctc agttcaacac gctggcgaac aacggctacg tacaggatgc ggatgcgttt
    64501 gcggagcaac tggtggttga gcgtgacagt gacaacccga accgcgtgaa cgtggtgtgg
    64561 ccgggccgcc tgatgaacca gttacgcatc tttgcggtgc ggaaccagtt caggctgaac
    64621 agcagcagtg attaaccatg atgccgcctg cgggcggctt ttttacggga gacgatgatg
    64681 agtaagaacg cgttagcggg cacctgtacc gtgacgattg acggcgtgtc ggtcaatgtg
    64741 gccgggacgt tcagatattc cgtgggtgaa atcgagcgtg aaacgctcac gggaatgagc
    64801 ggcatacatg gctttaagga gtcgtacaaa gcgccgttta tcgagatgac ggtacgtgat
    64861 tccggctcac tgtcactgaa ggattttgcg gcatatacgg atgtgacggt ggcggcttat
    64921 ctggtgaacg gcaagaccat tctcggccag aacatgtggc tggttggccg gattgagaca
    64981 gacaacaacg atgcaacctt tacggcccgt tttgaagggc aaacggtgtc ggagagctga
    65041 tgatgaatga gaaacagaca gaagaactgg tggtgaccct gaaacgtccg gtaagggatc
    65101 cggataccag gacggagttc cgtgagctgc gcctgaaaga gccggtgctg ttccaggcgg
    65161 aggactttca ccgcaatacc gcctcactgg gcgcaatggc ggccatgcgt gagctgattg
    65221 cggcagtggc gggtgtgccg tctgccccgc tgaagttcat ggcggtgtcc gattacaaaa
    65281 agtgcgaacg gttcctgacg ggttttttcc tggaagcctg agccagtggc aggcgagggc
    65341 ggctgaactg acgtggttct ttcgctggtc cccgcgcgag gcgtgggggc tgacgtcagg
    65401 ggaaattctc tggtgggcgt cgcaggcagc gcggatcaac agactgagga gtggcgatgg
    65461 cgggtaatgc tttcaatttt gagctgaatg cacgggataa cgcgagtgcg cttctgcaga
    65521 gagcctcaga ggctgccggg tcgctggcgg ataacgcaga gcaggcggcc gcctgcgtgt
    65581 cgtcactgtg tgacggtctt gatgcggtga atgaaacgcc cctgagcggg gcatccggtg
    65641 cggcagatgc gctgggcggg aagataacgc agatccacga ctcggtaacg ctactgatga
    65701 atgcgctgct ggccacagac agtgcgggca aaagggcgct gggaaaggag agtcaggaga
    65761 atgcggacag gatgtccgga tattttgaac gtctgtcccg gctggggaaa gacacatcac
    65821 agcacttcgg ggatattgtg ccgccgctgc gcaatgtcgg ggcactgtca tcggagctgt
    65881 tcagcgcact ggggcgcgga ggtcttgtgg ggcttacggc tgccgtcggt ggtaaggcgg
    65941 tgaaggcggt cgtcagtcat ctttatgatg cctcgaaagc ggcatacagc cttgatgtga
    66001 acgcaagaaa cgccggaatg agtgtgagtg cgttcagccg gtttgccggg gtgttccgcc
    66061 tgatggggct gtcggcagag caggcgcagg cggagaccgg cgcactgttc acgacactga
    66121 acgatgcgct gaacacccgt tcaccggagg tggtgggcat actgaatcag tttggcgtga
    66181 agctggcgga gaacagcaac cacacggtga atcttgagaa aagcacgcag aacctgactg
    66241 acgccttcgg gcggatgaac agctcatcgc agaaggtggt ggcggacgca ctggggctgt
    66301 cagatgcaca actggcgctg ctgcggggaa caaaaaatct gaatgcggcg ctggcggagt
    66361 ccgatcgcct gaaactgacg atgccggaca gcctgaacgc aaagctggtt caggcgaaca
    66421 gcaacctgaa ccttctgtct gctgcgtggg acggcttcac tgacagagtg aaggcgaaag
    66481 tgctgggcag tgatgccgtc accgcctctg ttgactgggc ggttgacgtc atgagcgaca
    66541 ggcgcagcag ccagcagaaa acgggtgatc atctgtcaga gctgcgcagt tatctgtatg
    66601 cgcatccgga gaaactgaag tcactgacgt cgcggcagag atacaatctc gataacaata
    66661 ttgcgacatc cgatcttctg gagatgtata cacggctgtc cggtcagcgg tccgacaggc
    66721 tgaatgcgct cacggcaacg ctgagtgatg acctggcagc ggcgctgtcg gcgccgtcac
    66781 aggcgatatc acaacctccc tcatcatcac cgcaaggtgg tacaggtcac aacgacatca
    66841 cccctgaact gctggcacat tttgcggcgc tggagaagca gtacaacctt ccggcaaaca
    66901 cgctgtacgg tctggccatg acggagtcgt cgggacgggc ggatgcggtc gggccgctca
    66961 cccgttacgg tgcggcaaag gggatgtttc agttcatcga cccgacggcg cgggagttcg
    67021 ggctttccgg catggatgtg tttaaccctt accgggcctc ggaggcggcg gccagaaaac
    67081 tggcggggct gatggcgcag tatggtggtg acatgcagcg cgcctttcag gcgtacaact
    67141 ggggcgaagg caacatgagt gcctggcttc gcggacataa ggggatgccg caggagacgc
    67201 aggcctacgc gccgaaggta ctcaggcaca tgtcacagat atccggcggc agcatcatca
    67261 cgccgcagtc cggcagttca gtggcagacg tgcgggatga aatcagtcgc ggtttcagcg
    67321 aaaacaaaat ccggctggac attaacgtca ccaacacgac cacggggcag acaacccgcc
    67381 ggacggtgaa gggaggcgcg gtgtccacgg cgatggatgt gtgaggagca gaaggtgggc
    67441 attatcacag acgcggtggc ggacacgctg gggctgtctt ccaccggcgg gtgggactgg
    67501 cagtcgcata ttaatcaggc gtccttccgc ggtgtgccgt ttggcgtcat cagcggtgag
    67561 agcgtggtgg gccgccgggt ggcggtacat gaatatccgt accgggatac ggtgtgggtg
    67621 gaagacctgg gacgcagtgc ccggaagttc acgctgcgtg ggttcctgat acaggacagt
    67681 ctggtgtaca gcgccggaga tgtgttcagc cagcgtgacg cgatgattgc ggcctgcgag
    67741 acatccggtg gtggtctgct ggtgcatccg acgctgggtg agatgacggt gtatgtgccg
    67801 gacggcgggc tgcgtattga agagggggtg gagtccgggc gggtattttc gttcacgctg
    67861 accgtcattg agtcaggtga aaaagcgttt tcactggtga cggggacaag ctccacgtca
    67921 tcggagacgt ggtatcagac gctgaccaca acggccgccg tgatgctggc ggcgattacc
    67981 ggtgagatga acagtgtcac cggggcggtt aagacgataa agagcacggt gagcgcgtgg
    68041 gagacgatat tttcgcgggc ggtgacatcg gtgaccagta tgaccagcac ggtgtcgtcc
    68101 ctgttttctc cggacagcta cgggcgttac tgcacagcca gtgacagcgg gaccgcatca
    68161 ggcagcacgg cctcgtcact ttccacgtgg cttgccacca cggaggcgga tgatgacagc
    68221 attctggaaa ccattcaggc ctgctccgtg cgggaccggt cggcggcgga atcggcgata
    68281 gcggcgctgg atgacatctc gtccgtatcc ggtgctgtgt ccgcgataca ggcggtcatc
    68341 accacgctgg cggaggccac gggcagtgac acggagaaaa tccgcgtcat ggcagagatt
    68401 gcgtcggcgg aagacgggac gtattacgaa ggggagacgg cctgcgccat cagtgcatcc
    68461 gtgcaggcct tcatccgcac cctgggcgcc ggtgcgatgc tgtggcgact gatgcagtac
    68521 acccctcagg gttacgatga tgcggtgtcg gtgatgcgga aggcccgcac ggtgacagag
    68581 gcggtactgc ttctgcttgc tgatgccgga gatgacgaca gtcacgatgc gctgaatgcg
    68641 cagtacacgc agtttgtcac gcactggcag acgaattatc tgtcacaaca ggacgtgatg
    68701 aaggtgacca gccgctcacc gcagccgtcg ctggcgctgg caaaccggct gtatcaggac
    68761 gccgcgcgtg cggacgaact ggtacaggcg gcctctcccg tccatccggc gttcatgccg
    68821 ctttccttca cagcgagaaa ctcatgagta caaccacaga cgacaccctg acactgaagg
    68881 taaacaacac cgtcatgtcg gggtggaatg atatccgggt gacgcggggc gtagagcgtc
    68941 tgccgtcgga ttttgagctt tcgctcacgg actggtatcc gcaggaagga taccagcttg
    69001 ccccgccggg gagcagctgc acgataacca tcggcgacga ccgggtgatg accggctatg
    69061 tcgaccagtg ggtgaacacc ctgacgccgc agtcgcatga catccgggtg acgggcaggg
    69121 ggatgtgtca ggacctggtg gactgctccg cctggtggga gaacaacatg ataaagggcg
    69181 gtgatgcgct ggccatcatc agaaaactgg cgtcggtgta cggcatcacg gtgacgacag
    69241 acgtcgacag tttcaccacg gtgccggatt ttgtcatcaa ctggggggag tcgtcgcagc
    69301 aaattatcga ccgcatctgc cggtatgagg ggcttctgta ttacgacctg ccggatggca
    69361 gcctgttcct gacgcgtgcg ggaacggcgt cggcagccag cggggtgaca cagggggtga
    69421 acctgcagaa ggcggagtac acacgaagta tgaatgaacg gttttcggaa tacaccggcc
    69481 tgtcggtgtc agtgaacagt atcagcgaac tgtcgccggc atccggttac gacagcgtga
    69541 tgctggcaac ggcccgtgac ccggaagcgg ccagtatgcg cacccgccgt cacgtcacga
    69601 tagtggagag cacgctgatg acgacggggt gtgcacagca ggcggtgaac tgggaaatga
    69661 accggcgcta cgggcgctca atggccctga atgtgacggt ggacagctgg cgtgacagtg
    69721 caggggtgct gtggcagccc aacacctttg tgccggtgag catacctgca ctgggggcgg
    69781 acagtctgaa ctggctggtc tctgaggtga cgttcagccg tgacgatgag acggggacca
    69841 cggcatcgct ggttctgatg ccgccggaag cgttcagcgt gcagccgtac cgtttttatt
    69901 ccggcgtggc cggaagggat gtgtcgtcat gaatcctgta caggtgcttt tccgccgtct
    69961 ggtgtcactg ctgagcgtgg ggcgcgtgac ggcgggggat gacagtggtg tggtgcagac
    70021 cgtgcaggtg cagagtccgt cagaggtgcg cagcgacacg ccggttcttc agcagtttgg
    70081 attttcctcg gtgctgcctg ccggaacgga tgtggtggtg atgagccttg ccggtaaccg
    70141 cagcagcgcg gcggtggtgg cgtccggcca tcagtcatac cggattaacg gtctgagcag
    70201 cggcgaggtg gtggtgtaca accagtgggg gcagtacgtc cggctgggtg aggatgggat
    70261 tgtggtggag gcgtcagggc agcaggtgtt ggtgaacagt gccaccacgc ttgaggtgac
    70321 ggcgacggac ggggtcaccc tgaaaacgcc gtcgctgaag gtgacggggg atatcacgga
    70381 caactgcgaa agcaacagca ccacgctgaa ggcgctgcgg gaggcataca acgaccacac
    70441 acatccggtc agcggtgtgg aaagtggcgg cagcacggtg acaagtcagg caaccacgga
    70501 gacggtgtga gatgagcgat atcacgcttt tttacgatga agaggcgctg cattgcgact
    70561 ggtcggtggg gacgggcgat atggtctgcg gaaatgacct gcagacggcc attctcatca
    70621 gcctgttcac ggaccggctg gcggatgcat cagatgagac ggacgacggc aaccggcgcg
    70681 gatggtgggg tgaccttgag caggattacc gggtcggctc ccggttgtgg ctgctgcgca
    70741 ggcagaaact gaccacgcag gtggcgctga aagcggaggc atacgccctt gaagcgctgc
    70801 agtggctgaa ggacgacggc gtggtggctt cactggatgt ggaggcgtgg atagtgccgc
    70861 cggaccggct gtacatgacc atcgcatatc agcgtccgga tgcggacacg acggaatacc
    70921 agaaatttta tcaggtgtgg gaggtatgac atgccgtttg aacgccccgg attaagtgag
    70981 ctgcgggaga aaagccggtc gtatgtgacc ggtcagctgg atgaggcggg ggctttgctg
    71041 cggttttcca cgccggggat tctggcggat gccgtggcgg gcatgacaca tctgcattac
    71101 ggctatcttg actggatagc gcagcagtgc acaccggcca cggccaccgg ggaatatctg
    71161 gcggcgtggg gcgcgctgaa gggtatcatc cgcaaggccg cagaggccgc ggcctgtgac
    71221 gaggtgcagt tcacgggaac cgtcggcagc accgtgagtg cgggggcggt actgaaccgt
    71281 gcggacgggt atcagtacac gctggatgag gatgtgagca ttgacagtga cggcagcggt
    71341 tcagggagta ttaccgccgt gctgccggac ccgacggatg acagcagcgg cggtggcgac
    71401 gacggtaacg cggatgccgg tacgacactg acaccggatg tcacctggtc cggtattgac
    71461 tcaacggtga cgatggtgac ggctgccacc ggcggcagtg acactgagga agaggagacg
    71521 taccgtcagc gtgtgctgta tgcgtaccag aacccgcctc agggcggtgc ggcggcggac
    71581 tatgtgcagt gggcactgga ggttcccggc gtgacccggg cgtggtgcgt gaaccgtgcg
    71641 ctggggttcg gaacggtggg tgtctacatc atgacggacg gcgacgacga ggacaacgcc
    71701 gggggctttc ctgacggcac ggacggcgtg gcaacggatg aggactggac agaccggaaa
    71761 gccacggggg tacagctcac ggtggcggac tatatccgcg attaccagcc ggtcacggcg
    71821 gtggtgtatg tgatgtcgcc ggtggccaga agcattgatt ttgaaataca ggggctgagt
    71881 gatgccacgg cggcactgag ggcacaggtg gagagcgcca tcagcgaggt gctgtacaac
    71941 gtggatgaac tggacggcag cggggtgatt aacctttctg acctgtatta cgccattgcg
    72001 gacattgagg ggacagacgg cttcattctg gtgtctcctg acagcaatat cacgctgtcg
    72061 cagggagagc tgccggtcac cggaagtatc acatgggcaa catgaggaaa atcatgagtc
    72121 gtttttcaca tgatgactac acgtgcgcga tggtgaatct tctgccatcg gggatggcgt
    72181 ggaaccggct gcctgacagc gtgcagtaca ggctgatgcg cgggctggcg caggcgtacc
    72241 ggcagagtga tgctgatgcg tgcgccctga taacgggggc cttcccggaa acggcggatg
    72301 cgcttactga tgagtggtat gcctcgctgg ggctgaatga tgaatgcgga acgcaggcat
    72361 ccaccacaga cccggcgcag gccagaaaat tcattctggc gaaactgctg tcgacgggcg
    72421 ggcagagcgt ggcgtatttc acggagctgg cggcgacgat gggctacagc atccgtatcc
    72481 gtgagtaccg cacaccgctg tgcgggtttt cgtggtgcgg gcacctgctg agtgatgaca
    72541 accggttcaa ctggaccgtg gtggtggccc cgcaggggga tgacgtgacc acctcacggg
    72601 cgtacctgga gtgcctgttc cgtcgttatg ccccggcaca gacgctggtg acgtttgagt
    72661 ggcagtcgtc atacagcgcc gtcctgagta tggcgtggga caacagcacg cgccttcttt
    72721 caggtgcact gatggcggat gagggtgtgg tggtgtcggg cgtgactgtg aatgtcgtca
    72781 tcaaatccat gtcgggacag cggtatgtgg cgacggtgtc cacagacagt gacgggggct
    72841 ggtcgtttga agcggatgat gaaaccttca caaatggcag ttacacggtt tatgcgcagg
    72901 cggacgtgga tatgccggat gatgtcacgg tgcctgtacg aagcgcgtta ctgaatgtac
    72961 gggtacagac acagggtgag ggggacaacg gtgatgatga gccggcatct gacgacacga
    73021 cgcaggagcc gctggtcatt aaccgggtga tgcttcagtc ggggcgcacg acgcaggagc
    73081 cgctgattat cagcagggca caccttcagt cgggataccc gacgcaggag ccgcttgtca
    73141 ttaacagtgc gatgcttcag tcgggatatc tgacgcagga gccgcttgtc attaacagtg
    73201 cgatgcttca gtcgggatat ctgacgcagg agccagtggt cattaacagg ctctggctga
    73261 attttgccgt cactaatgag tgacaacaac gaaaggagac tgtttttgaa ctcaatcata
    73321 cttgaaggat tccggcagat agcggcggat gaggcgctgt gcgacagcta cacttcagac
    73381 aacattcttt ccgcactgaa caaaaacggc gtcacactta tcaccgaaat gtcaggttcg
    73441 gtgagttctg tgtgccgcac cacgcagacc atcaatacgg tggcgcgtac cgggctggca
    73501 ttcacgacat catcaaacct catgctcagg tgtctgctgc ctgatttgag tgagacggcg
    73561 gtgcagttcg ggtttcgcct gtcactgggg gagggagaga agtattcctc ttctccgtct
    73621 cacatcggga ttggcagcgc atcgttcacc accccgtatc cggatgaagc cgccagctac
    73681 tattttgaga tttttgctgc ctggaacggc agcagcacgg tcagtgccag tctgtactgt
    73741 aaccgcgcgc tggtcgggag cgcgactctc ggtgtgtcat cgtcaggaac aggggcggtg
    73801 agtgtccgga ttggcggtgg caacaatgta tttgcatcag gtgtgagcgg cattttcatg
    73861 ccgggtgata tgtactgcat gacggtgccg tatggtgata actatgatgc ctcaccatca
    73921 ctgctgggga gtattgaggt cggctacagt ccggtaacgg cattcaccgg ggggagtgcc
    73981 acaaactcgc tgagtaaaga tattgtcacc gggctgaacg catctgatga cgacagcggg
    74041 tatctgatac tgaacagttc aggtgaagcc gccggggtca cgtttgagga tattgataac
    74101 accgggggaa ccctggttgc ggtgcaggcg gcggtcaccc tgagggaatc cgcttcgccc
    74161 aacaatcttc tggcatggag tgtggggtgc ggaagcagca ggggcgatgt gatatcagaa
    74221 acggaggcag aaagcgacac gaacagctgg acgacggtga cgcaggggtt catgagtatg
    74281 ccgggagatg gcagcgagtt tgaaacaggc gcactgacct tcacggcgaa tctgtataac
    74341 cggaagaaaa gtgaacagga gacggcctga tgagaaaaat atcggacatc acatcatatg
    74401 tggatgagaa tgacgagttc acggacggtg agccagccac aggcgtgaag ccgacgccgt
    74461 tgcttgctct ctggttcaac gtcatacagc gggagctggt gaaggtggtg gagagtgcgg
    74521 ggctgacact ggacccgaca gatgacacac agctatggca ggcgctgtcg ggctattttg
    74581 cctcacagga gaatgtgaac accacaatca gcgcgctggg cacgatggct tcccagaaca
    74641 gtgatgcggt ggatatcacc ggcggcgcga taacaggcac ctccctcacc ggtgacctga
    74701 cgggcaatgc ggacacggcc acaaaactga aaacggggcg gaaaatccgg acaaatcttg
    74761 acagcacgag tgctgtcagt tttgacggca gtgcgaatgt cacgccgggc gtgaccggca
    74821 cattgccggt gagcaatggc ggtacggggg ccacgacggc atcagacgcc agaacaaatc
    74881 tggagctggg agatgtggca acccagagtc tgagtacgct cgatgcccgt tacctgctga
    74941 cgacaggagg tgtgtcggcg gtaaggctgg ggagtgtcag ttcatatagc cctcccggca
    75001 acgagtcgtc gtggacgcag aatctttcct ccggcaatgt gctcacaggg attatcgttc
    75061 aggatacagg gtccaattct gcggataaca ttggcggaat ttattacagg caggttcagt
    75121 actgcataaa cggcacatgg ataaatgcgg tatcactgta aaggagagtg ttaatgataa
    75181 acatcaaaaa tttcacgcca tacatcccgg aaaacccgcc cgtggcgtgg gcgtcatatc
    75241 tcatcagcga ggatggtcag gactggtatg aatgccagac gcagtttgca gaggatacat
    75301 acaaagttgc gtacggcagt gacggtatcg tgcgcagcat ttcgaccgat gtgtcggcac
    75361 tttttccgct caacatgagt gttgctgagg ttgagtcgct gccggacggt gcggatattg
    75421 acggcaactg ggtattagac ggggagagtg tggttgcgcg gacgctgacg gcagcggagt
    75481 ggcaggcaaa ggcggaatca caacgaagcg cgctgatatc ggatgcgaag gcgacaatca
    75541 gcctctggca gtcagagctt ctgctgggca tcatcagtga tgacgacaaa gagagtctga
    75601 cggaatggct gtcttacata aaggcgcttc aggggctgga tttaagtggt gtgacagatg
    75661 aggagggtta caacgcgaca gtctggccgg atgaaccggg agccacagca taaaagccgc
    75721 tgagaagcgg tttttttaac aaaaaaagcg gcacataaag tgccgcaaac gcattgtttg
    75781 tgagttttag gccttaatac ctttgacgcc tttcattcaa cagaaaaaga atttctttgt
    75841 caattttata ttatatgttg tgttgctttt tgttgttttt ttgattttta tttttatatt
    75901 attgcacgtt tttgattaaa actttgctgg atgtttgtgc gcgttataga aagctgaaga
    75961 tgaataaatg ttttctttaa aagaaaaacc ggcacggttg atgccggtta tatcaaggag
    76021 tgatataaaa tgggatgaaa ggattggaaa aaagaactta atcaaaaggc atcgtaggat
    76081 caagcgaata tgcaaatatc gttgtttttt tattgaatta ctatattttt tgtaacgatt
    76141 gatggtccat ggttcgagag tgcattgtcc ttcggtgaaa gaaaaatccg aaacattgat
    76201 cctacccacg taatgtggac acg
//
LOCUS       DAHBHC010000013        75636 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHBHC010000013 DAHBHC010000000
VERSION     DAHBHC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.013"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            5..145
                     /locus_tag="M2A35_001162"
     CDS             5..145
                     /locus_tag="M2A35_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCB7197487.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(548..847)
                     /gene="ihfA"
                     /locus_tag="M2A35_001163"
     CDS             complement(548..847)
                     /gene="ihfA"
                     /locus_tag="M2A35_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCB7197488.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(852..3239)
                     /gene="pheT"
                     /locus_tag="M2A35_001164"
     CDS             complement(852..3239)
                     /gene="pheT"
                     /locus_tag="M2A35_001164"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCB7197489.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHSLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVVPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(3254..4237)
                     /gene="pheS"
                     /locus_tag="M2A35_001165"
     CDS             complement(3254..4237)
                     /gene="pheS"
                     /locus_tag="M2A35_001165"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCB7197490.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(4447..4571)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(4521..4565)
                     /gene="pheM"
                     /locus_tag="M2A35_001166"
     CDS             complement(4521..4565)
                     /gene="pheM"
                     /locus_tag="M2A35_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCB7197491.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(4688..5044)
                     /gene="rplT"
                     /locus_tag="M2A35_001167"
     CDS             complement(4688..5044)
                     /gene="rplT"
                     /locus_tag="M2A35_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCB7197492.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(5097..5294)
                     /gene="rpmI"
                     /locus_tag="M2A35_001168"
     CDS             complement(5097..5294)
                     /gene="rpmI"
                     /locus_tag="M2A35_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCB7197493.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(5391..5933)
                     /gene="infC"
                     /locus_tag="M2A35_001169"
     CDS             complement(5391..5933)
                     /gene="infC"
                     /locus_tag="M2A35_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCB7197494.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(5937..7865)
                     /gene="thrS"
                     /locus_tag="M2A35_001170"
     CDS             complement(5937..7865)
                     /gene="thrS"
                     /locus_tag="M2A35_001170"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCB7197495.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            8389..10287
                     /gene="espL1"
                     /locus_tag="M2A35_001171"
                     /pseudo
     CDS             8389..10287
                     /gene="espL1"
                     /locus_tag="M2A35_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10463..10570
                     /locus_tag="M2A35_001172"
     CDS             10463..10570
                     /locus_tag="M2A35_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197496.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(10623..11381)
                     /locus_tag="M2A35_001173"
     CDS             complement(10623..11381)
                     /locus_tag="M2A35_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCB7197497.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            11668..12597
                     /gene="pfkB"
                     /locus_tag="M2A35_001174"
     CDS             11668..12597
                     /gene="pfkB"
                     /locus_tag="M2A35_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCB7197498.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISATQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12698..12988
                     /gene="ghoS"
                     /locus_tag="M2A35_001175"
     CDS             12698..12988
                     /gene="ghoS"
                     /locus_tag="M2A35_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCB7197499.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            13094..13954
                     /locus_tag="M2A35_001176"
     CDS             13094..13954
                     /locus_tag="M2A35_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCB7197500.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(13995..14531)
                     /locus_tag="M2A35_001177"
     CDS             complement(13995..14531)
                     /locus_tag="M2A35_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCB7197501.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            14678..15346
                     /gene="hxpB"
                     /locus_tag="M2A35_001178"
     CDS             14678..15346
                     /gene="hxpB"
                     /locus_tag="M2A35_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCB7197502.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            15509..16099
                     /locus_tag="M2A35_001179"
     CDS             15509..16099
                     /locus_tag="M2A35_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCB7197503.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            16232..17623
                     /gene="tcyP"
                     /locus_tag="M2A35_001180"
     CDS             16232..17623
                     /gene="tcyP"
                     /locus_tag="M2A35_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCB7197504.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(17627..18004)
                     /locus_tag="M2A35_001181"
     CDS             complement(17627..18004)
                     /locus_tag="M2A35_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197505.1"
                     /translation="MKKIIYILPGAFLLRFKKNESLLKFPSIISMLNINLLFNPLNLP
                     GMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESV
                     CIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(18074..18442)
                     /locus_tag="M2A35_001182"
     CDS             complement(18074..18442)
                     /locus_tag="M2A35_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197506.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     MGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINENFVGMDFTCEF"
     gene            complement(18719..18982)
                     /gene="cedA"
                     /locus_tag="M2A35_001183"
     CDS             complement(18719..18982)
                     /gene="cedA"
                     /locus_tag="M2A35_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCB7197507.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            19165..21426
                     /gene="katE"
                     /locus_tag="M2A35_001184"
     CDS             19165..21426
                     /gene="katE"
                     /locus_tag="M2A35_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCB7197508.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDSTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(21473..22235)
                     /gene="chbG"
                     /locus_tag="M2A35_001185"
                     /pseudo
     CDS             complement(21473..22235)
                     /gene="chbG"
                     /locus_tag="M2A35_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
     gene            complement(22248..23600)
                     /gene="celF"
                     /locus_tag="M2A35_001186"
                     /gene_synonym="chbF"
     CDS             complement(22248..23600)
                     /gene="celF"
                     /locus_tag="M2A35_001186"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCB7197509.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(23705..24547)
                     /gene="chbR"
                     /locus_tag="M2A35_001187"
     CDS             complement(23705..24547)
                     /gene="chbR"
                     /locus_tag="M2A35_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCB7197510.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(24555..24905)
                     /gene="chbA"
                     /locus_tag="M2A35_001188"
     CDS             complement(24555..24905)
                     /gene="chbA"
                     /locus_tag="M2A35_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCB7197511.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(24956..26314)
                     /gene="chbC"
                     /locus_tag="M2A35_001189"
     CDS             complement(24956..26314)
                     /gene="chbC"
                     /locus_tag="M2A35_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCB7197512.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(26399..26719)
                     /gene="chbB"
                     /locus_tag="M2A35_001190"
     CDS             complement(26399..26719)
                     /gene="chbB"
                     /locus_tag="M2A35_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCB7197513.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKLVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(27018..27356)
                     /gene="osmE"
                     /locus_tag="M2A35_001191"
     CDS             complement(27018..27356)
                     /gene="osmE"
                     /locus_tag="M2A35_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCB7197514.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            27558..28385
                     /gene="nadE"
                     /locus_tag="M2A35_001192"
     CDS             27558..28385
                     /gene="nadE"
                     /locus_tag="M2A35_001192"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCB7197515.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            28615..29502
                     /gene="cho"
                     /locus_tag="M2A35_001193"
     CDS             28615..29502
                     /gene="cho"
                     /locus_tag="M2A35_001193"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCB7197516.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(29462..30037)
                     /gene="ves"
                     /locus_tag="M2A35_001194"
     CDS             complement(29462..30037)
                     /gene="ves"
                     /locus_tag="M2A35_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCB7197517.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFHWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(30240..30725)
                     /gene="spy"
                     /locus_tag="M2A35_001195"
     CDS             complement(30240..30725)
                     /gene="spy"
                     /locus_tag="M2A35_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCB7197518.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(31054..32022)
                     /gene="astE"
                     /locus_tag="M2A35_001196"
     CDS             complement(31054..32022)
                     /gene="astE"
                     /locus_tag="M2A35_001196"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCB7197519.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(32015..33358)
                     /gene="astB"
                     /locus_tag="M2A35_001197"
     CDS             complement(32015..33358)
                     /gene="astB"
                     /locus_tag="M2A35_001197"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCB7197520.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(33355..34833)
                     /gene="astD"
                     /locus_tag="M2A35_001198"
     CDS             complement(33355..34833)
                     /gene="astD"
                     /locus_tag="M2A35_001198"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCB7197521.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(34830..35864)
                     /gene="astA"
                     /locus_tag="M2A35_001199"
     CDS             complement(34830..35864)
                     /gene="astA"
                     /locus_tag="M2A35_001199"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCB7197522.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(35861..37081)
                     /gene="astC"
                     /locus_tag="M2A35_001200"
     CDS             complement(35861..37081)
                     /gene="astC"
                     /locus_tag="M2A35_001200"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCB7197523.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            37527..38333
                     /gene="xthA"
                     /locus_tag="M2A35_001201"
     CDS             37527..38333
                     /gene="xthA"
                     /locus_tag="M2A35_001201"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCB7197524.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            38500..39210
                     /locus_tag="M2A35_001202"
                     /pseudo
     CDS             38500..39210
                     /locus_tag="M2A35_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            39215..39892
                     /gene="ydjY"
                     /locus_tag="M2A35_001203"
     CDS             39215..39892
                     /gene="ydjY"
                     /locus_tag="M2A35_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCB7197525.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            39907..40614
                     /locus_tag="M2A35_001204"
     CDS             39907..40614
                     /locus_tag="M2A35_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCB7197526.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            40614..41162
                     /locus_tag="M2A35_001205"
     CDS             40614..41162
                     /locus_tag="M2A35_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCB7197527.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            41172..42338
                     /locus_tag="M2A35_001206"
     CDS             41172..42338
                     /locus_tag="M2A35_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCB7197528.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            42311..43846
                     /locus_tag="M2A35_001207"
     CDS             42311..43846
                     /locus_tag="M2A35_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7197529.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            43846..44499
                     /locus_tag="M2A35_001208"
     CDS             43846..44499
                     /locus_tag="M2A35_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCB7197530.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            44566..45873
                     /gene="ynjE"
                     /locus_tag="M2A35_001209"
     CDS             44566..45873
                     /gene="ynjE"
                     /locus_tag="M2A35_001209"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCB7197531.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(45882..46502)
                     /locus_tag="M2A35_001210"
     CDS             complement(45882..46502)
                     /locus_tag="M2A35_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCB7197532.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            46589..46996
                     /gene="nudG"
                     /locus_tag="M2A35_001211"
     CDS             46589..46996
                     /gene="nudG"
                     /locus_tag="M2A35_001211"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCB7197533.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(46962..47234)
                     /locus_tag="M2A35_001212"
     CDS             complement(46962..47234)
                     /locus_tag="M2A35_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCB7197534.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            47470..48813
                     /gene="gdhA"
                     /locus_tag="M2A35_001213"
     CDS             47470..48813
                     /gene="gdhA"
                     /locus_tag="M2A35_001213"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCB7197535.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(48930..49971)
                     /locus_tag="M2A35_001214"
                     /pseudo
     CDS             complement(48930..49971)
                     /locus_tag="M2A35_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(50099..52060)
                     /gene="topB"
                     /locus_tag="M2A35_001215"
     CDS             complement(50099..52060)
                     /gene="topB"
                     /locus_tag="M2A35_001215"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCB7197536.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(52065..53108)
                     /gene="selD"
                     /locus_tag="M2A35_001216"
     CDS             complement(52065..53108)
                     /gene="selD"
                     /locus_tag="M2A35_001216"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCB7197537.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLNEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(53225..53776)
                     /locus_tag="M2A35_001217"
     CDS             complement(53225..53776)
                     /locus_tag="M2A35_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCB7197538.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            53937..55793
                     /gene="sppA"
                     /locus_tag="M2A35_001218"
     CDS             53937..55793
                     /gene="sppA"
                     /locus_tag="M2A35_001218"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCB7197539.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            55960..56976
                     /gene="ansA"
                     /locus_tag="M2A35_001219"
     CDS             55960..56976
                     /gene="ansA"
                     /locus_tag="M2A35_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCB7197540.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            56987..57628
                     /gene="pncA"
                     /locus_tag="M2A35_001220"
     CDS             56987..57628
                     /gene="pncA"
                     /locus_tag="M2A35_001220"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCB7197541.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(57789..58061)
                     /locus_tag="M2A35_001221"
     CDS             complement(57789..58061)
                     /locus_tag="M2A35_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCB7197542.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(58103..58516)
                     /gene="msrB"
                     /locus_tag="M2A35_001222"
     CDS             complement(58103..58516)
                     /gene="msrB"
                     /locus_tag="M2A35_001222"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCB7197543.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            58858..59853
                     /gene="gapA"
                     /locus_tag="M2A35_001223"
     CDS             58858..59853
                     /gene="gapA"
                     /locus_tag="M2A35_001223"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCB7197544.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            59937..60821
                     /locus_tag="M2A35_001224"
     CDS             59937..60821
                     /locus_tag="M2A35_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCB7197545.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(60872..61726)
                     /gene="yeaE"
                     /locus_tag="M2A35_001225"
     CDS             complement(60872..61726)
                     /gene="yeaE"
                     /locus_tag="M2A35_001225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCB7197546.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(61816..62562)
                     /gene="mipA"
                     /locus_tag="M2A35_001226"
     CDS             complement(61816..62562)
                     /gene="mipA"
                     /locus_tag="M2A35_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCB7197547.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            62998..64932
                     /gene="yeaG"
                     /locus_tag="M2A35_001227"
     CDS             62998..64932
                     /gene="yeaG"
                     /locus_tag="M2A35_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCB7197548.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            65045..66328
                     /locus_tag="M2A35_001228"
     CDS             65045..66328
                     /locus_tag="M2A35_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCB7197549.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            66475..67950
                     /locus_tag="M2A35_001229"
     CDS             66475..67950
                     /locus_tag="M2A35_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCB7197550.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            68131..69621
                     /locus_tag="M2A35_001230"
     CDS             68131..69621
                     /locus_tag="M2A35_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCB7197551.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            69664..70167
                     /gene="yeaK"
                     /locus_tag="M2A35_001231"
     CDS             69664..70167
                     /gene="yeaK"
                     /locus_tag="M2A35_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCB7197552.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGALPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(70168..70272)
                     /locus_tag="M2A35_001232"
     CDS             complement(70168..70272)
                     /locus_tag="M2A35_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197553.1"
                     /translation="MNDQMFVETLIISSSFFAIAVVLVLSVLLIERTG"
     gene            70442..70888
                     /locus_tag="M2A35_001233"
     CDS             70442..70888
                     /locus_tag="M2A35_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCB7197554.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(70845..71666)
                     /gene="nimR"
                     /locus_tag="M2A35_001234"
     CDS             complement(70845..71666)
                     /gene="nimR"
                     /locus_tag="M2A35_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCB7197555.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVIMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWACFFAMSERNIARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            71763..72944
                     /gene="nimT"
                     /locus_tag="M2A35_001235"
     CDS             71763..72944
                     /gene="nimT"
                     /locus_tag="M2A35_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCB7197556.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            72999..73346
                     /locus_tag="M2A35_001236"
     CDS             72999..73346
                     /locus_tag="M2A35_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCB7197557.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(73368..73622)
                     /gene="yoaF"
                     /locus_tag="M2A35_001237"
     CDS             complement(73368..73622)
                     /gene="yoaF"
                     /locus_tag="M2A35_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCB7197558.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            73805..74830
                     /gene="dgcP"
                     /locus_tag="M2A35_001238"
     CDS             73805..74830
                     /gene="dgcP"
                     /locus_tag="M2A35_001238"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCB7197559.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            74863..74961
                     /locus_tag="M2A35_001239"
     CDS             74863..74961
                     /locus_tag="M2A35_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCB7197560.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            74964..75038
                     /gene="yoaJ"
                     /locus_tag="M2A35_001240"
     CDS             74964..75038
                     /gene="yoaJ"
                     /locus_tag="M2A35_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCB7197561.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(75097..75345)
                     /locus_tag="M2A35_001241"
     CDS             complement(75097..75345)
                     /locus_tag="M2A35_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCB7197562.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
BASE COUNT        18943 a        19314 c        18146 g        19233 t
ORIGIN      
        1 cgctatgtcg caaaaatcgc ttacggccat cacgttctgc gtgacggcaa tcctcatcat
       61 ctggatgcta cacggttcgc tgtgtgagat acggatgagc ttctggggag cggagtttgc
      121 ggcgttctta caatgtaagc agtaagaaaa ccgcgacggg gaaggcaact tccccgtcaa
      181 tcggttgcca gggttaaggc cgacaaggca ccctatacac atcccaaagc agaaacccgc
      241 agcgtaaaaa ctgcgggttt tctttttggt accctcattc atgaggacac cagaaacaaa
      301 gcccacaaca caggaactgt gggctttttg cattcaaaga tggacgttat atggacacat
      361 gaaaataaaa catgtttatt ttcagttaat tacaccacaa cttaaagcgc ccgcaggtgc
      421 atcatcaaaa agttacctta cactaaaaat gtggccattt agtggaaaac aaaaataaaa
      481 ggcattacaa atcaatatgt taaccaaata aaaaaggccg cagagcggcc tttttagtta
      541 gatcagatta ctcgtctttg ggcgaagcgt tttcgacccg gctttttaac ttctgcccgg
      601 gtctgaaggt caccacgcgc cgtgctgtaa tgggaatatc ctcgcccgtt ttcgggttac
      661 gtcccgggcg ttgattctta tcacgcagat cgaagttacc aaaaccagag agtttcacct
      721 gttcgccgtt ttccagagcg cgacggatct cttcgaaaaa cagttcaacc agttctttgg
      781 catcccgctt gctaagccca agcttatcaa acagatattc tgacatttca gcttttgtaa
      841 gcgccatagg ttcaatccct caatgatgcc tggaatcgct cttttaatgc ctctacacat
      901 ttggcgacgg tagcggcaat ctcctcttct tcgagtgtac ggctggtatc ttgcaggatc
      961 aggcttatgg cgaggctctt atacccctcc gcaacaccct taccgcggta cacgtcaaat
     1021 aagtttacgc caactacctg atttacgcca actttcttac attcggataa aatatccgct
     1081 gcgggaacgt tttctgcgac caccaccgcg atgtcacgac ggttcgccgg gaagcgagaa
     1141 atctcgcgcg cctgaggcac cacgcggtct gcgagcttgt tccactccag ttcgaacacc
     1201 agagtgcgac cgttaagatc cagtttacgt tccagttcag gatgaacaac cccaacaaaa
     1261 ccaatacgtt cacctttcag ataaatcgct gcggattgcc ccggatgcag tgccggattc
     1321 gcttctgcac ggaactcaac ctcattcagt ttaccggtca ggtcgagaac ggattcaaga
     1381 tcgcctttca aatcatagaa atcaacggtc tcttttgcca ggttccagtg ctcttcgtaa
     1441 cggttaccgc aaatcacacc ggctaacatc agatcctgac gaatgcccaa cggtgcctga
     1501 gtatctggta cgaaacgcag accgctttcg aaaatgcgca cacggttctg ctgacggttc
     1561 tggttgtaca ccacggttgc cagcaggccg gtccacagag aaagacgcat tgctgacatt
     1621 tcaacagaga tcgggcttgg cagcagtaag gcttcaacgc ctggatggat catctgctgc
     1681 actttcggaa caacgaagct gtaggtgatc acttcctgat agcctttgtc gttgagcagc
     1741 gttttcacgc gcttgagcga caggtcagct tcacggtgag tacccataat caggcttgcc
     1801 tgtaccggct catccgggat gttgttgtag ccgtaaacac gcgcgacttc ttcgaccaga
     1861 tcttcttcaa tctccatatc gaaacgccag ctcggcgcaa ctgcctgcca ctcgtctttg
     1921 ccttcggtca cttcgcagcc gagacgacgc agaatgtcag ttacctgctc atccgcaata
     1981 tgatggccga tcaggcgatc cagtttgcta cgacgtaaag tgatggttgc acgcttcggc
     2041 agcgttgctt cgttggtgat atcaattacc ggaccagcct caccaccgca gatgtcgatc
     2101 agcagacggg tcgcacgttc catcgctttg tgctgcagtg ccggatcaac gccacgctca
     2161 taacggtgag acgcatcagt atgcaggcta tgacgacgag cacgaccggt gatagacagc
     2221 gggctgaaga aagcgcattc cagcaagacg ttttgcgttt cgtcattcac gccagagtgt
     2281 tcgccaccaa agatgccgcc catcgccagc gccttgttgt ggtcggcgat gaccagagtg
     2341 tcagcattca gcttcgcttc agtgccatca agcagtacca acgtttcgcc ctctttcgcc
     2401 atccgcacca caatgccgcc ttcaatgcga tctttatcga aagcgtgcat cggctggccc
     2461 agttcgagca gtacatagtt ggtgacgtca acaactgcat cgatagaacg gatcccgcaa
     2521 cgacgcagtt tttctttcat ccacagcgga gttggcgctt taacgttaat gccttttacc
     2581 acacggccaa gataacgcgg gcaggcttcc ggcgcttcga ctgtaatcgg cagcgtgtcg
     2641 tcgatggtcg caccaaccgg aacgatttcc ggttcaacca gcggcagctg attcagcacg
     2701 gcaacgtcac gcgcaacacc aatgatacct aaacagtcgg cacggtttgg cgtcacgctg
     2761 atttcgatgg tgttgtcatc aagtttcagg tattcacgga tgtcggtgcc aatcggcgca
     2821 tccgcaggca gctcgataat gccgctatga tcgtcagaaa ttcccagctc agaaaaggag
     2881 cacagcatcc cttcagacgg ttcaccacgc agtttcgccg ctttaatttt gaaatcaccc
     2941 ggcagaacag caccaatggt agctaccgcc acacgcaggc cctgacggca gtttggcgca
     3001 ccgcagacga tgtccagcag gcgatcgccg ccaacattca cttttgtcac acgcagtttg
     3061 tcagcgttcg gatgctgcgc acactcaacc acttcaccaa cgaccacgcc gtggaagcta
     3121 ccggcaaccg gttctacacc gtcaacttcc aggcccgcca tagtgatttg attcgccagc
     3181 gcatcgctat caatcgccgg gttcacccat tcgcgtaacc acagttcact gaatttcata
     3241 atctattcct gccttattta aactgtttga ggaaacgcag atcattttcg aagaatgaac
     3301 gcaggtcggt gacgccgtaa cgcaacatag tcagacgctc catccccatc ccgaaggcga
     3361 aaccagagta aacttccggg tcgatgccaa cgttacgcag cacgttcgga tgcaccatcc
     3421 cgcaacccag cacttccagc catttaccgt ttttacccat gacgtcaact tctgcagaag
     3481 gctcggtgaa cgggaagtag gaaggacgga agcgaatctg caaatcttcc tcaaagaagt
     3541 tacgcaggaa atcgtgcaga gtgcctttca ggttggtaaa gctgatgttg gtatcaacaa
     3601 tcagaccttc catctgatgg aacatcggcg tgtgagtctg gtcgtagtcg ttacgataaa
     3661 cgcggccagg cgcaatgata cgaatcggtg gctgctgggc tttcatggtg cggatctgta
     3721 cgccagaggt ctgggtacgc agcaggcggg tagtgtcaaa ccagaaagtg tcgtggtcag
     3781 cgcgcgccgg gtggtgacca ggaatgttca gagcatcgaa gttatgataa tcgtcttcga
     3841 tttccggccc ggttgccacg gtaaagccaa gctcaccgaa gaaactttcg atacggtcga
     3901 tagtacgggt aaccggatgc agaccgccgt tttcaatgcg acgacctggc agagagacat
     3961 caatcgtttc cgccgccaga cgcgcattca gtgcagcgct ttccagttcc gctttacgcg
     4021 cattcagcgc ctgctgaacc tgctctttcg cttcgttgat aaccgcacca gctgccggac
     4081 gctcttctgg cggcagctca cgcagggtcg tcatctgaag ggttaagtgc ccttttttac
     4141 ccaaatattc gacgcgcaca ttatctaacg cggcaacatc tgacgcctgg ctaatggccg
     4201 ccttcgcact ggcaaccagt tctgcgagat gtgacatggt tttcctcatt gtgtcagtgg
     4261 tgacactggt tcgttggact tagagcctat cccatcaggc tattttactt gccattttgg
     4321 tcccgggcag tgctcgaaat tctcacgtac ttaaatacgc tccggtttct ccgcgctggc
     4381 cgtgtccaga ctggctgcga caattacacc tgatgagaca ggctttttat ttttcaaaac
     4441 gcgcatacaa aaaaagcctc cactgggagg ctttcaggcg ctgttttccg tttctcttct
     4501 cacgcgctag cctcctggat tcaggtgcta aagtaaaaaa agaagcggaa aatagcagca
     4561 ttcattgctt gcgttacctt ttggtactct tcaaaagacc tttattgaaa aggctacggc
     4621 gataaaagtc aatgttttga tggcgttgaa acgaaaagag ggagactagc tccctctttc
     4681 aactggctta tgccagagct gctttcgctt tttcaaccag agcggtgaac gctactttgt
     4741 cgaatactgc gatatcagcc aggatcttac ggtcgatttc aacagaggct tttttcaggc
     4801 cattgatgaa tttgctgtaa gaaataccgt tctgacgtgc tgctgcgttg atacgcgcaa
     4861 tccacagttg acggaactga cgcttacgtt gacgacggtc acggtaagca tactgaccag
     4921 ctttgataac agcctggaag gcaacgcggt atacgcgaga acgcgcaccg tagtagcctt
     4981 tagcttgttt caaaattttc ttgtgacgtg cgcgtgcaat aacaccacgt tttacgcgag
     5041 ccatatgtgc tctcctgtat ctatattcta attaaaaagt taaaaacgtt aacggcttat
     5101 gcgtacggca ggcacgcgat taccaggccc agatcgcctt tggaaaccat ggctttcgga
     5161 cgcaggtgac gtttacgttt ggtcgctttt ttggtcagaa tgtgacgcag gttagcgtgc
     5221 ttgtgcttaa aaccaccttt accggttttt ttgaagcgct tagcagcacc gcgtacggtc
     5281 ttaatttttg gcattttaat aacttccact tcgcattgtt aataaacgaa acaaaggcga
     5341 acaaagcctg tgaagcccga aggctccaca gacagtgcta cttgaaggcc ttactgtttc
     5401 ttcttaggag cgagcaccat gatcatctgg cggccttcga tcttcgttgg gaaggattcg
     5461 accactgcca gttcttgcaa atcgtctttc acgcgattaa gcacttccat accgatttgc
     5521 tggtgcgcca tctcacgacc gcggaaacgc agcgtgattt tggctttatc accctcttcg
     5581 agaaagcgaa tcaggctgcg gagttttacc tgatagtcgc cttcatctgt accaggacgg
     5641 aatttaattt ccttaacctg gataactttt tgctttttct tctgttcctt agaagacttg
     5701 ctcttttcat agaggaattt gccgtaatcc attatacgac aaaccggcgg ctcggcgtta
     5761 gggctgatct cgactaagtc tactccggct tcttctgctt tctccagagc ttctctcaga
     5821 ctcacaatac caagctgctc gccttccaga cctgttaagc gaacttcctg ggcgcgaatt
     5881 tcgccattga tacggttagg gcgcgccgtt tgaactcgtt ttccgccttt aataccttat
     5941 tcctccaatt gtttaagact gcggctgcga atctcttgtt gcagcttctc gatcacttca
     6001 tttacgtcca tgcttcccag gtctttacca cggcgggtgc gaacggcaac tttgcctgat
     6061 tccacctctt tatcaccaca gaccagcata tatgggacgc gacgcaaagt gtgctcgcgg
     6121 attttaaagc caatcttctc atttctcaag tctgctttaa cacgaatgcc cgcatttgat
     6181 agtttttgcg tcaattcgtt aacgtattca gactgtgaat cggtaatatt catgataaca
     6241 acctgaactg gcgcaagcca ggtcgggaag aaaccagcga attcttcggt caggataccg
     6301 atgaaacgtt ccatcgatcc cagaattgcg cggtgaatca ttaccggtac tttacgttca
     6361 ttgtcttcgc ctacataaga agcgctcaga cgagaaggca aagagaagtc cagctgtact
     6421 gtaccgcact gccatgcacg atcgaggcag tcatacaggg taaattcaat tttcggaccg
     6481 tagaaagcgc cttcacccag ttgatattca aacgggatgt tgttttcttc cagcgcaacc
     6541 gccaggtccg cctcagcacg atcccacatt tcatcactgc caatacgttt ttcaggacga
     6601 gtggagagtt tgacgacgat cttctcgaag ccaaaagtgc tgtacatatc atagactaaa
     6661 cggatacatc cgttaacttc atcgcgaatt tgttcttcag tacagaagat atgcgcgtca
     6721 tcctgggtaa atccacgcac gcgcatcagg ccatgcagcg aacctgacgg ctcattacgg
     6781 tggcagctac caaactcggc catacgcagc ggcagatcgc gataagactt cagcccctgg
     6841 ttgaaaattt gtacgtgacc cgggcagttc atcggcttaa tgcagtattc acggttctca
     6901 gaagacgtgg tgaacattgc atctttgtag ttgtcccagt gaccggtttt ttcccacagg
     6961 acacggtcca tcatgaacgg acctttaact tcctgatact ggtactcttt cagtttagaa
     7021 cgaacaaaca cttctagttc acggaagatg gtccagccgt cgttgtgcca gaataccata
     7081 cccggcgctt cttcctgcat atggtacagg tcgagctgtt taccgatttt acggtggtcg
     7141 cgtttcgcgg cttcttccag gcgctgcagg taagcgttaa gcgctttttt gtctgcccac
     7201 gccgtaccgt aaatacgttg caacattttg ttgttgctgt cgccacgcca gtaagcccct
     7261 gccgttttca ttagtttgaa atgatggcag aaacgcatgt tcggtacgtg cggaccgcgg
     7321 cacatatcga catattcttc atggaagtac agacctggct tgtcatcatg ggcgatgttt
     7381 tcgtcaagaa tggagacttt gtagctctcc ccacggttgg cgaaagtttc acgcgcttcg
     7441 tgccagctga ctttcttctt aatgacgtcg tagtttttct cagcaagctc atgcatccgc
     7501 ttctcgagtg cttcgacatc ttcctgggtt aacgtgcggt caagatcaac gtcgtaataa
     7561 aaaccgttgt caataaccgg gccgattgcc attttggtat gcggccaaag ttgtttaatc
     7621 gcgtgcccta acaggtgcgc acaggagtga cgaatgatct ccagaccgtc ttcgtctttg
     7681 gcggtaatga tggacagttg tgcgtcgttt tcaatcagat cgcaagcatc aaccagttcg
     7741 ccattaacgc gccctgcgat acaggctttc gccagacctg gaccaatgtc cagcgcaaca
     7801 tccatggggc ttacagcgtg gtcgtaatgg cgttggctgc catcaggaag agttataaca
     7861 ggcattttat atccttattt gcagtggtga cccacacgaa agatcacata caaagaaaaa
     7921 tttgtttatt aacagttcat tgcgaaacca tctagccaac aaatgctaaa ttggttcgca
     7981 atcaggtaca caaatgaaag acattaacct ctcaccttcc acccggttga gcgatgttaa
     8041 cactaaaaaa agggagattg tacctttccg tttcacattg attcgattcg caattcgttt
     8101 gtagtgagta caccagcaaa acgcttaaag aaccactgat aacatcacgg tgcgtctgaa
     8161 aggtttactg cgagcggatt tacagcagca taaactgaat ccagcaaagt tcgttgcgtg
     8221 atttggcggt agtattttaa acgctcatga gattaatgac gaagtggtca tatcacaatg
     8281 ataaaagtga cacaattctt ataacaattt ttcgtgcaca tttcgttctg gcgataataa
     8341 ttaatcatca tcctcaaaac ctccagatat atataaggcg aatagattat gggtcaaaac
     8401 gatatcatta tcagaactca ttataagtct cctcatagat tgcacatcga tagcgacata
     8461 ccaacacctt catcagagcc tattaataaa tttgcgcgcc agctcatcac cctacttgat
     8521 acctctgact taagttcgat gctgtcatac tgtgttattc aggaatttac cgcaaactgt
     8581 cgaaaaatat cacaaaattg ttattccact gcccttttta ccattaactt tgccacttca
     8641 cccatccatg cagaaaatat actcattaca ttacactata aaaaagattt catttcctta
     8701 ttactggaaa ccacgcctat taaagctaac catttgcgaa gcatactgga ttatattgaa
     8761 caggaacagt taactgctga agatcgtaac cattgtatga aactgtctaa aaaaatccat
     8821 agagaaaaaa ctatacaccc aacagtaaat ctcaatggta gtgcatgttt tttgcaatct
     8881 ccttctgacg ctattttttg tcgccatctg tcattgcaat acgcccttga ttcattgaga
     8941 aatggaaaag gcaaagtcaa cctgattaaa cattactcct ccgttgaatc catacagcag
     9001 catatcccct tagtccggga tgcggagttc agagcattac ttcgccatcc tcctgcaggg
     9061 agtcgcgtta tcgcgagtaa ggattttggc ttcgctttag atattttctt ttgtcgaatg
     9121 atggcaaaca atgtcagtca tatgtccgcg attttatata tagacaatca tactttgtca
     9181 gtaaggctac gaataaagca gtcagtgtat ggacaattaa attatgttgt gtctgtttac
     9241 gacccgaacg ataccaacgt tgccgtcaga ggcacccaca ggacagcacg gcgctttctc
     9301 tcgcttgata agttcatcag ttcagctccc gatgctcaga cctgggctga taggtatgtt
     9361 cgcaactgtg caattgctat tcttcctcta ttacctgagg gagttccagt tgctatttta
     9421 gcgggtatca cgacacgaat gccatttgcc cctatacatc catcggcaat gttgttaata
     9481 atggccacag gccagactca atagcttatt acactattca aacagttgcc catactccct
     9541 gaaaaagaaa tcattgaaat aataactgcg cagaatagcg ttggtacacc tgctttattt
     9601 ctagctatga tgaacggaca aactgacaac gtaaaaatat ttatgcaaga aatccagtca
     9661 ctggtataca atcatatcat tcatgaagat aatctggtta aattactgca aactaaaagt
     9721 gctaacgaaa cacctggact ctatatctcc atgttgtatg gattcgatga aataatcgat
     9781 atctttctga atgcattaac cactccaata gcacaagaac ttttaaacaa aaaaatggtg
     9841 atggatattt tagccatgaa aacacgtgat ggtgaaccag ggttatttgc tgcaatggaa
     9901 aataatcacc ctttgtgtgc cacacggttc ctctctaaag tttatgggat cgccgttaaa
     9961 tataaactca gcaaaatcaa catcatggac ttattaaaag gcgcgacagc acatggaacc
    10021 cctgctttat acatcgccat gagcaagggt aataaagacg tcgtgttatc ttatatatca
    10081 acactgagta cttttgcaaa aaaatattct tttagtcaac gtcaattatt cacactgttg
    10141 gccgctaaaa atcatgaaaa catgtcagct gttcatatag ccattcatca taatcattat
    10201 aaaactgtag aaacatatta tgctgctatt aatgcaatca gccaaagcct gagttttagt
    10261 gctgatgaac taaaaacgta tttataacca gctatttatg atatttgtat atcgatacat
    10321 aattttattt ttttcataaa acgcagaaac agtgatcagg tgtacaacgc cacccgacgg
    10381 ctgtaataac aaaaaatccc ttgatgcctg tcccttttgt tacactccgt tatcacgcac
    10441 aagagatatg caggacactg gtatgccgac taaacgcttt gataaaaaac actggaagat
    10501 ggtggtggtg ctactggcaa tctgtggcgc tatgttgttg ctacgttggg cagcaatgat
    10561 ttggggctga gcagtgacaa acgaagacag ccagaccgca taacacggtc cggcagataa
    10621 ttttacatgc tgtagcccag cgataaggtt gtccgacgat cggtatgttc cggtgcagac
    10681 tctggaggtt cagagttcca tgtgacgttg taagccactt tcagtccaaa atgttcatta
    10741 atagcaacat ttaatgcgct ttcggagttc agcgttgtgt cttccgcgcc aaagacggaa
    10801 acaccctgcg taaatttagc gttgtcagtc aactgccatg cataagcacc ggaggcatag
    10861 cccagcggct gagtttcact ggcattgtcg gtatatttgt cgtaacgcac accaggaccg
    10921 aattcaaagc ggaaactgtg taccgggcca ttcaaaaact gacgaccata acccgcggtc
    10981 aacacatcgc gctctcgata gccgttataa cggtctgtca gccagcttgc ctgaccaaat
    11041 aaatagtcat aatcagttaa attaaaacgg ctacgcccgc ccgccgcata tttttctgaa
    11101 gaacgctcat cattagaaga agtattactg gcgttccccc acagcgacca ggcagtggtt
    11161 tgtccatacc aggtcatggt ggtgtcagcc gtaagtgagg agcttttcgt attgcctgat
    11221 tgtgcaagat atcctgcgtt cagattacct tcgaaaggtt ttttagcgct ggcaggatcg
    11281 tccatgacag taaaaacgga atcatcggca gctgcattca gtgacgcaaa catgcccccc
    11341 gccaacataa cgatggcagg aactgtcttc aaaagcttca tttatcaaga gtccgtacaa
    11401 caaaaaaaga gaccatcgcg gtcccggaaa ctttcttaag gatcaaagat tagcgtccct
    11461 ggaaaggtaa cgaattataa aaaggcgcga ataacttagc aatgtattct tatttcattt
    11521 tttgaataag catgtggcga aaacagattt ttatttatat atatttatct gcaaaatttt
    11581 aaataaagct ctaataaatc atattgttaa tttcttcact ttcggctgat tcggtgccag
    11641 actgaaatca gcctatagga ggaaatgatg gtacgtatct atacgttgac acttgcgccc
    11701 tctctcgata gcgcaacaat taccccgcaa atttatcccg aaggaaaact gcgctgtacc
    11761 gcaccggtgt tcgaacccgg gggcggcggc atcaacgtcg cccgcgccat tgcccatctt
    11821 ggaggcagtg ccacagcgat cttcccggcg ggtggcgcga ccggcgaaca cctggtttct
    11881 ctgttggcgg atgaaaatgt ccccgtcgct actgtagaag ccaaagactg gacccggcag
    11941 aatttacacg tacatgtgga agcaagcggt gagcagtatc gttttgttat gccaggcgcg
    12001 gcattaaatg aagatgagtt tcgccagctt gaagagcaag ttctggaaat tgaatccggg
    12061 gccatcctgg tcataagcgg aagcctgccg ccaggtgtga agctggaaaa attaacccaa
    12121 ctgatttccg ctacgcaaaa ccaagggatc cgctgcatca tcgacagttc tggcgaagcg
    12181 ttaagtgcag cactggcgat tggtaacatc gagttggtta agcctaacca aaaagaactc
    12241 agtgcgctgg tgaatcgcga actcacccaa ccagacgatg tccgcaaagc cgcgcaggaa
    12301 atcgttaata gcggcaaggc caaacgggtt gtcgtttccc ttggtccaca aggagcgctg
    12361 ggtgttgata gtgaaaactg cattcaggtg gtgccaccac cggtgaaaag ccagagtacc
    12421 gttggcgctg gtgacagcat ggtcggcgcg atgacactga aactggcaga aaatgcctct
    12481 cttgaagaga tggtccgttt tggcgtagct gcggggagtg cagccacact caatcaggga
    12541 acacgtctgt gctcccatga cgatacgcaa aaaatttacg cttacctttc ccgctaacaa
    12601 aaacattccc ccagcattgg gggaatcacc accaacctgt cggcaacgcg tttctccgac
    12661 tatgctcaaa agtcatgtga taacaaaggg gtgaactatg gccagtggcg atcttgtccg
    12721 ttatgtcata accgtcatgt tgcatgagga tacattgact gaaattaacg agttgaataa
    12781 ttacctgact cgcgacggtt ttttgctcac catgacggat gatgagggaa atatccatga
    12841 gctggggact aacacttttg gacttatcag tacccaaagt gaagaagaaa ttagagaact
    12901 ggtttcgggg cttacccaaa gtgcaaccgg caaagatcct gaaatcacca tcacgacctg
    12961 ggaggaatgg aatagcaaca gaaaataaat ggtttttggg caataatcag tctgtggtgt
    13021 gcgttagctc gtgtttttac accgcattct tgcgctaacc ttatgatctg gcagacaaca
    13081 tgggagagac atcatgtggc aggcaatcag tcgtcttttg agcgagcagt taggtgaagg
    13141 cgaaatcgaa ctgcgtaatg aactgcctgg cggagaagtc catgccgcat ggcatttgcg
    13201 ctatgcagga catgactttt tcgtcaaatg tgatgaaagg gaactgcttc ccggttttac
    13261 cgccgaagcc gaccaactgg agttactgtc gcgtagtaaa accgtcaccg tgcctaaggt
    13321 ttgggcagta ggcgctgacc gtgactacag ttttctggtg atggattatc tcccacctcg
    13381 tccgctggat gcgcatagcg catttattct tggtcagcaa attgcgcgtt tacatcaatg
    13441 gagtgaccaa ccacaatttg gcctcgattt tgataactcg ctctccacaa ctccacagcc
    13501 caacacctgg caacgtcgct ggtcaacgtt ttttgctgaa caacggattg gctggcagtt
    13561 ggaactggca gcagagaaag ggatcgcttt cggcaatatc gacgccatcg tcgagcatat
    13621 tcagcagcgt ctggcctcgc atcaaccgca gccttctctg ttgcacggcg atttatggtc
    13681 cggcaactgt gcgctgggtc cggatggccc gtacattttc gaccctgcct gctactgggg
    13741 tgaccgagag tgcgacctgg cgatgttacc gctgcatact gaacagccgc cacaaatcta
    13801 tgacggctat cagtcagtat ccccgctacc tgccgatttc cttgaacgtc aaccggttta
    13861 ccaactctac acgctgttaa atcgtgcaag attatttggc ggtcagcatt tggttattgc
    13921 tcagcagtca ttggatagat tattagcagc atgatatggg ttgaggataa tggccgctcc
    13981 gtgcggcctt ttgattaaat aaacccaagc agagaaaaga acacataacc tgcagcaata
    14041 caaataaccg gcaggatata tagtgagaaa aactgcagga aaatagtatg gtgcggaaca
    14101 acaatacggg actcaatttg ttcacgcgtt agcccctctt cccctttggc tttttccaga
    14161 atgagttgat cttcaacacc ttcccgcaag aagcgcgcct ggcgactcat ccgggcaccg
    14221 gaatcctgca acgccagtcc gacaaaaatc aggatgaaaa tcacccagaa cataacgttc
    14281 acgccaccat taaaattagg ggtcggggag ttataccaga acagattcaa aaaaggcgta
    14341 ttcgcctgca tcatatcgat catgacatga gtaaagtcga gcatgaccgc attaatgcct
    14401 tcctggtttt cctgccgggt attcataaat ttcagcaaag aaatcaatgt agagatcaat
    14461 gcaggtataa aaatcaccca acccaaaatc cttttcaaaa cagcaatgcg tccagcttgt
    14521 tgatacgtca tgagttctcc ttgattaaga cgcgtcgttt cacttagttt acctgtagat
    14581 atctgttttc gcccattctt taaaggcgat atgataggcg cttaatcata agcacggctt
    14641 aataccttac acataatgct ctaaaggaga ggttgtaatg tcaaccccgc gtcagattct
    14701 tgctgcaatt tttgatatgg atggattact tatcgactca gaacctttat gggatcgagc
    14761 cgaactggat gtgatggcaa gcctgggggt ggatatctcc cgtcgtaacg agctgccgga
    14821 caccttaggt ttacgcatcg atatggtggt cgatctttgg tacgcccggc aaccgtggaa
    14881 tgggccaagc cgtcaggaag tagtagaacg ggttattgcc cgtgccattt cactggttga
    14941 agagacacgt ccattattac caggcgtgcg cgaagccgtt gcgttatgca aagaacaagg
    15001 tttattggtg ggactggcct ccgcgtcacc actacatatg ctggaaaaag tgttgaccat
    15061 gtttgactta cgcgacagtt tcgatgccct cgcctcggct gaaaaactgc cttacagcaa
    15121 gccgcatccg caagtatatc tcgactgcgc agcaaaactg ggcgttgacc cactgacctg
    15181 cgtagcgctg gaagattcag taaatggcat gatcgcctct aaagcagccc gcatgcgttc
    15241 cattgtcgtt cctgcgccag aagcgcaaaa tgatccacgt tttgtattag caaacgtcaa
    15301 actctcatcg ctgacagaac tcaccgcaaa agaccttctc ggttaatgac caggggcagt
    15361 gatctcgctg cccctggttc tttatctgaa tttgcattca actgacggat taatcgtcaa
    15421 tttaagagaa agagttacac cgtcaccact tccgtgcact gtataaaaat cctatactgt
    15481 acgtatcgac agtttagtga gttttatcat gacggcggaa ggtcaccttc tcttttctat
    15541 tgcttgtgcg gtatttgcca aaaatgccga gctgacgccc gtgctggcac agggtgactg
    15601 gtggcatatt gtcccttccg caatcctgac gtgtttgtta ccggacatcg atcacccaaa
    15661 gtcgtttctt gggcagcgat taaaatggat atcaaaaccg atcgcccgcg cttttgggca
    15721 tcgtggtttt acccacagtc tgctggcggt atttgcgctg ctggcaacct tttaccttaa
    15781 ggttccggaa ggctggttca ttccggctga tgcgctacaa ggaatggtgc tgggttattt
    15841 gagccacata cttgccgata tgctgacacc cgccggtgtt cccctgctct ggccatgccg
    15901 ctggcgtttc cgcttgccta tcctggttcc ccaaaagggc aaccaactgg aacgttttat
    15961 ctgcatggca ttatttgtct ggtcggtatg gatgccccat tcattacccg agaacagcgc
    16021 tgttcgttgg tcatcgcaaa tgatcaatac cttgcagatc cagtttcatc ggcttattaa
    16081 gcatcaggtt gaatactaaa aagacaaaaa tcacctttct ggaataagca attccatttg
    16141 aatataagag ccagctcaca gttctgttaa tcttgcgcca acactatgac tgctacgcag
    16201 tgatagaaat aataagatca ggagaacggg gatgaacttt ccattaattg cgaacatcgt
    16261 ggtgttcgtt gtactgctgt ttgcgctggc tcagacccgc cataaacagt ggagtctggc
    16321 gaaaaaagtg ctggtgggtc tggtgatggg tgtggttttt ggccttgccc tgcataccat
    16381 ttatggttct gacagccagg tacttaaaga ttctgtacag tggtttaaca tcgttggtaa
    16441 cggctatgtt caactgctgc aaatgatcgt tatgccgtta gtcttcgcct ctattctgag
    16501 cgcggttgcc cgtctgcata acgcatctca gttaggcaaa atcagttttc tgaccatcgg
    16561 tacgcttttg tttaccacgc tgattgcggc gctggtcggt gtgctggtca ccaacctgtt
    16621 tggtttgacg gctgaaggtc tggttcaggg tggtgcagaa actgcacgtc tgaacgccat
    16681 tgaaagtaac tatgttggta aagtctctga tctgagcgtt ccgcaactgg tcttgtcctt
    16741 tatcccgaaa aacccgtttg ccgatcttac cggagccaat ccgacgtcaa ttatcagcgt
    16801 ggtaattttt gccgcattcc tcggcgtagc tgcattgaaa ctgctgaagg atgatgcgcc
    16861 gaaaggtgaa cgcgtcttag ccgctatcga taccctacaa agctgggtga tgaaactggt
    16921 tcgccttgtc atgcaattga ccccttacgg cgttctggct ctaatgacca aagtggttgc
    16981 aggttctaac ttgcaagaca tcatcaaact gggaagtttc gttgtcgcgt cctacctcgg
    17041 tctgctgatt atgtttgcag tgcatggcat tctgctgggc attaatggcg tgagtccgct
    17101 gaagtacttc cgtaaggtat ggcctgtgct gacgtttgcc tttaccagcc gttccagtgc
    17161 tgcgtctatc ccactgaatg tggaagcaca aacgcgtcgt ctgggcgttc ctgaatccat
    17221 cgccagtttc gccgcctctt tcggtgcaac cattggtcag aacggctgcg ccggtttgta
    17281 tccggcaatg ctggcggtga tggttgcgcc tacggttggc attaacccgc tggacccgat
    17341 gtggattgcg acgctggtcg gtattgttac cgttagttcc gcaggcgttg ccggtgtcgg
    17401 tggtggtgca actttcgccg cactgattgt gctgcctgcg atgggcctgc cagtaaccct
    17461 ggtggcgctg ttaatctccg ttgaaccgct tatcgacatg ggccgtacgg cgctaaacgt
    17521 tagtggctcg atgacagctg gcacgctgac cagccagtgg ctgaagcaaa ccgataaagc
    17581 cattctggat agcgaagacg acgccgaact ggcacaccgt taattcttat gctggcaacg
    17641 gtccgttttg tataggggcc gttgctttac tttttaaatg ttccatcgcg gcgatgcata
    17701 ctgactcata tttaaacaga tcgtcagcga aaaaaatgtc caacttatca atacattcct
    17761 ggctcaatga atgtgcctgt agtgagaagc ttaaaacctc atacccgaga cgttttctaa
    17821 gtgaactatc tggaatactc ataatatctt caagcacacc acttcccatt cccggaagat
    17881 ttagtggatt aaaaagaaga ttgatattaa gcattgatat tatcgaagga aactttagga
    17941 gactctcatt ttttttgaat cgcagcaaaa atgctcccgg caggatgtat ataatttttt
    18001 tcatagtata tagtccttaa ataatgaata accaacctat ttttatattg atcaaattcg
    18061 tcattttttc gggtcagaat tcacaggtaa aatccatccc aacaaaattt tcattaatat
    18121 ctcttatccg aaactcactg ccataaagat tattaattat cactctatat ttaacattgt
    18181 cctgcagata cgtatcaaaa ctaagtgctg gcgctcctgg ctttgaggca tcatagttaa
    18241 tgttagacat ataggcaatc atacgattga agttcgactc tgctatttca tcgcagtcac
    18301 attgacccat tgatataaag aaagtgagtg ccgccatggt agactcaagc ctttctttaa
    18361 aagagtcgaa agccaggctt ttaatattta aatcaccata attactctgt attaagtttg
    18421 tagaaaacat ctcccgcccc atattgttaa caaaattatt atctcattta aatctaagtc
    18481 acttacaata taagtttaag agcgacgcca caggatgaac tatcaaaaat agctcatcat
    18541 gattagcaaa acttaaccat tttaaaataa ataaacaatt aaagaaaaaa gatcacttat
    18601 ttatagcaat agctcgtcaa aggcagcttt ttgttacagg tggtttgaat gaatgtagca
    18661 acgaaatgca gaatttcagg tcatgtaact cccggcaaaa ccgggaggta tgtaatcctt
    18721 actcagtcac ttccccttcc tggcggatct gatttgccca acgttgggca gattcaggca
    18781 cagtaaacgc cggtgagcgc agaaatgact ctcccatcag tacaaacgca acatatttgc
    18841 cacgcagcat ccagacatca cgaaacgaat ccatctttat cgcatgttct ggcggcgcgg
    18901 gttccgtgcg tgggacatag ctaataatct ggcggttttg ctggcggagc ggtttcttca
    18961 ttactggctt cactaaacgc atattaaaaa ttagaaaaac tgtagtttag ccgatttagc
    19021 ccctgtacgt cccgctttgc gtgtatttca taacaccgtt tccagaatag tctccgaagc
    19081 gggatctggc tggtggtcta tagttagaga gttttttgat caaaacagcg gccctttcag
    19141 taataaatta aggagacgag ttcaatgtcg caacataacg aaaagaaccc acatcagcac
    19201 cagtcaccac tacacgattc cagcgaagcg aaaccgggga tggactcact ggcacctgag
    19261 gacggatctc atcgtccagc ggctgaacca acaccgccag gtgcacaacc taccgcccct
    19321 gggagcctga aagcccctga tacacgtaac gaaaaactta attctctgga agacgtacgc
    19381 aaaggcagtg aaaattatgc gctgaccact aatcagggcg tgcgcatcgc cgatgatcaa
    19441 aactcactgc gtgccggtaa ccgtggtcca acgctgctgg aagattttat tctgcgcgag
    19501 aaaatcaccc actttgacca tgagcgcatc ccggaacgta ttgttcatgc acgcggatca
    19561 gcagctcacg gttatttcca gccatataaa agcttaagcg atatcaccaa agcggatttc
    19621 ctctcagatc cgaacaaaat caccccagta tttgttaggt tctctaccgt tcagggtggc
    19681 gctggctctg ccgataccgt gcgtgatatc cgtggttttg ccaccaagtt ctatactgaa
    19741 gagggtattt ttgacctcgt tggcaataac acgccaatct tctttatcca ggatgcgcat
    19801 aaattccccg attttgttca tgcggtaaaa ccagaaccgc actgggcaat tccacaaggg
    19861 caaagtgccc acgatacttt ctgggattat gtttctctgc aacctgaaac tctgcacaac
    19921 gtgatgtggg cgatgtcgga tcgcggcatc ccccgcagtt accgcaccat ggaaggcttc
    19981 ggtattcaca ccttccgcct gattaatgcc gaagggaagg caacgtttgt acgtttccac
    20041 tggaaaccac tggcaggtaa agcctcactc gtttgggatg aagcacaaaa actcaccgga
    20101 cgtgacccgg acttccaccg ccgcgagttg tgggaagcca ttgaagcagg cgattttccg
    20161 gaatacgaac tgggcttcca gttgattcct gaagaagatg aattcaagtt cgacttcgat
    20221 cttctcgatc caaccaaact gatcccggaa gaactggtgc ccgttcagcg tgtcggcaaa
    20281 atggtgctca atcgcaatcc ggataacttc tttgccgaaa acgaacaagc agcattccat
    20341 ccaggtcata ttgttcctgg tctggacttc accaacgatc cgctgttgca gggacgtttg
    20401 ttctcctata ccgatacaca aatcagtcgt cttggtgggc cgaatttcca tgagattccg
    20461 attaaccgtc cgacctgccc ttaccacaac ttccagcgtg atggcatgca tcgtatgggg
    20521 atcgacacta acccggcgaa ttacgaaccg aactcgatca acgataactg gccgcgcgaa
    20581 acaccgccgg gaccgaaacg cggcggtttt gaatcatatc aggaacgcgt ggaaggaaat
    20641 aaagttcgtg agcgcagtcc atcgtttggc gaatattatt cccatccgcg tctgttctgg
    20701 ctaagtcaga cgccattcga gcagcgccat attgtcgatg gtttcagttt tgagttaagc
    20761 aaagtcgttc gtccgtatat tcgtgagcgc gttgttgacc agctggcgca tattgatctc
    20821 actctggccc aggcggtggc gaaaaatctc ggtatcgaac tgactgacga ccagctgaat
    20881 atcaccccac ctccggacgt caacggtctg aaaaaggatc catccttaag tttgtacgcc
    20941 attcctgacg gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga tgaagtgaga
    21001 tcggcagacc ttctggccat tctcaaggcc ctgaaggcca aaggcgttca tgccaaactg
    21061 ctctactccc gaatgggtga agtgactgcg gatgacagta cggtgttgcc tatagccgct
    21121 acctttgccg gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg cggcaatatc
    21181 gcggatatcg ctgacaacgg cgatgccaac tactacctga tggaagccta caaacacctt
    21241 aaaccgattg cgctggcggg agacgcgcgc aagtttaaag caacaatcaa ggtcgctgac
    21301 cagggtgaag aagggattgt ggaagctgac agcgccgacg gtagttttat ggatgaactg
    21361 ttaacgctga tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga caaaattccg
    21421 gcgtaaatat ggataaggtc acgtcccctg gggagtgacc ttaccacctt acttacacat
    21481 taagataact acccaaccga taaccacgct cagcaattgc atatttcaac gacgctgatg
    21541 tcagcacttc cagctccgtt aaccgtggga aacagtaagc actctgacgg atagtattat
    21601 cgataaacgc cggatgacac atcacctcca gcgaacgatc accccgatgg cttgcgtcat
    21661 ccagcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg ctactgaatc
    21721 cctgagaact gcgcagatta gccggtaaat caccagcgtt agatagcggc tgacggtcga
    21781 tgcgcaacgc aatcccctct tcagccgcaa acctggcaac aatcgggaaa atctgcggga
    21841 acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat agctcaatga
    21901 aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa gcatcttctt
    21961 ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc ggccggcata
    22021 gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc ctatggccag actcggttca
    22081 tcacgactca actgcaccgc atggtcaata gcctgcccat tcaccagcgc cgtcgttgat
    22141 gtgacaatcc cattgcgaca ggcctcgata atgccgtagt tctgaccttt gcttaagcca
    22201 aaatcatcgg cattaacaat cagtaagcgt tccataatca gcctcggtta atgtgctttt
    22261 ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc
    22321 tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca acggactaag gtttagcgcc
    22381 agtaacacat cgttaaattc tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct
    22441 ttaatggtgt gaatcagccc catcacttta tcatcgaaat gcgtaatgcg cggatgtggc
    22501 gtcgcgccat cgcgccccag cttacaggtc atttctaccg cccagtctgc cggaatatta
    22561 tcaatatgcc catgatgcgg gatattaacg taatgttcag cttgcttgtc gttgtagata
    22621 gcgttgatca cttcgcacgc tgcatcagag taataagccc caccgcgctg ttccagttct
    22681 ttcggcttaa ctttcaactc cggattttta tacagctcaa aaagttgttt ctcgactttc
    22741 tgtactacct gtgctcgtgc gccgcctttg tagtattcgc ccatttcaat agccagcatc
    22801 tctttctgct tgaagtaata cagcagataa gaacatggca gcagatttaa cgaacgaatt
    22861 aagccctcac taaatggcag atcgaaaata ttttttacag aggacgcttt taactgccct
    22921 gacgccacac catcaagcaa ttcggcaaag cgcgacttgc catttaccag cacatcctta
    22981 atgaacacca tatggttgag gccgaacaga tcgatagata aatcatcgct gtctttcagc
    23041 atcagaacat cgcgaataaa catcttcatg ccgatcggaa tattacacac gccgataaag
    23101 cgtttaaatc cggtatgacg ataaacggct tcagtgacca ttcccgccgg gttagtgaag
    23161 ttaatcaccc atgcattcgg acaaagttct tcgacatctt ttacgatgtc aaaaatcacc
    23221 ggaatggtac gcagaccttt aaacagaccg cccgcgccgt tggtttcctg accaagataa
    23281 ccatgactta atggaatacg ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt
    23341 tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc gatccagcgt tttataaagc
    23401 ttcatcggga cgccagcgtt atcaatcatc cgttggcaga gttcaaaaat gatatccagt
    23461 ttctctttac catcttcgac atccaccagc cataattcgc tgaccggcaa ttcgtgataa
    23521 cgcttaataa atccttccag taactccggg gtatagctgc tcccgccacc aatagtgacg
    23581 acttttaatt tctggctcat aatttctccc ttcagtacag aatactgata tctggcatat
    23641 ctgccccccc ggacataaat aatccagcaa caggacagat atgtgaattg tcaggtataa
    23701 cgacttactg attaaattca gtcaatttct tacgatagct cttaggcgta aaggacgtta
    23761 atttcttaaa cgttttaata aacaaactcg ggctactata accggcctca aacgcaatat
    23821 ccgtcacgga atagttggtc atttccagtt gttttttggc aaaattaata cggatctcat
    23881 taataatctg cattggtgtt ttgccataat atcgttgagt cgctcgcgtc aaatattcct
    23941 gtgatttggc tgacaacgtc accatattct ccagcgccga ttcactaaac tgctctttat
    24001 catgcatctt ttctaccgta cttttcagcc actgcggtac atcatcaatc acctgttctt
    24061 cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac catctcaaca aactcttcca
    24121 gccctgtttc gcggaaattc aatgaagaaa tcactgtttc cacataggtg agaaaagcgt
    24181 tattggtccg gtatacctgc gaagcgacaa agcaataagg caacaacggc aggtaatgct
    24241 gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg cgtggcacca aactcataaa
    24301 aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc agtaacacgc
    24361 gcttaccgtt aatctcctgg aaataacgcc cggttaatac cagagtaaat tcataatagt
    24421 cgtgctgatg cagcccgctg atactctcag ttttgttata gataaacaca tggaagtttt
    24481 tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta atcactggct
    24541 gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc agtaatcagt
    24601 tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac cagactcact
    24661 ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag atgcgcttca
    24721 ttcaatgcca tccgtgactg atccatcatg gcttttgctg cagcaaaatc gccctgcttc
    24781 gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc cggagttgat gataagcccc
    24841 atcaccactt cttccagctc ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc
    24901 atcatacatc gttcctctct attcttaccg gcacgattac ccgtaccggc atcgattaaa
    24961 atttcagggc attagcgata tcttcttcgc tctcttcttt atcaatcgcg ttttgtgctt
    25021 tgttagccac cacaacaaag ggcagataaa ttaacgttgc gataccaagg ttgaagagtg
    25081 cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga ccggttggca
    25141 tggtccacgg tgcaatattg gtcaccggag gaatgatgcc catgtagtac gctgcgaggg
    25201 tgattgccgc cagaatcggt tgtaccagta caaacgggat aaacatcacc gggttcatga
    25261 taattggcag accaaacaga atcggttcgt taatctggaa gatgccggac ggcagcgcca
    25321 gttttgccac ctgacgataa tcagcacggc gagaggcgat aaagatagcc aggatcaggc
    25381 ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga
    25441 tatggaaggt cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg
    25501 cgatattttc cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg
    25561 cgccatgaat accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca
    25621 cgctacccaa cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg
    25681 tgccccaggt attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg
    25741 gaattaatgc cgagaaggaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga
    25801 cccaattgcg gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc
    25861 cggagatgat attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac
    25921 tatatggggt gacggtcata aatgctgcaa cggataacaa cccagccgcc agcgcatcga
    25981 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
    26041 ctaatgttcc gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg
    26101 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
    26161 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
    26221 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
    26281 gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat
    26341 ttaaatattg cggtattgat ttatgaaata actctttgac gggaaaattt aaaataaatt
    26401 aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga
    26461 ctttgccata aagcagcgag tcaattactt caaccagttt gttgggtaac aaacgctgga
    26521 tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt
    26581 tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg
    26641 cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat
    26701 aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc
    26761 cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt
    26821 gtggctgaaa ggagtatacg gtatagacct gagcaatagt atttctttga ccttataaat
    26881 tgtttctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag
    26941 ataattaaga actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa
    27001 gctttcagac gaattgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc
    27061 tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct
    27121 ttaccatcac gttgacccag gatgtaggtc tggcaagtac cgcgagcatg gatcatgctc
    27181 acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg
    27241 acgtctttca ccacaggctg tacaaactgg tctttagtac gatcataagc cgtacaaccc
    27301 gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc
    27361 cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc
    27421 ccgtgaacga aacagctccg ctttcagagg attcctgtat gacgttttaa ccaccattca
    27481 gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt
    27541 ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac
    27601 cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc
    27661 aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc
    27721 ttgccggaaa gctgtgccag atggcgatta atgagctgcg ccaggaaact ggcaacgaat
    27781 cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa caagattgcc
    27841 aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg
    27901 tattggctag cgagcaggca ttgcgggaag caggcattga actgagcgat tttgtccgtg
    27961 gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg
    28021 gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg
    28081 gtgacggcgg tacggatatt aatccgctgt atcgtctcaa caaacgccag ggtaaacagt
    28141 tgctgacggc attaggttgc ccggaacacc tttataagaa agccccaacg gccgatctgg
    28201 aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg
    28261 acgactatct ggaagggaaa aacgtacctg aacaggtcgc cagaacaata gagaactggt
    28321 atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa
    28381 agtaataatt tgcacctatt ggattgtgcg aaaaagagta atttgttcac gccggatgcg
    28441 gcgtgaacgc cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa
    28501 taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt
    28561 ttaatcgcct cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta
    28621 cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa
    28681 catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc
    28741 gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt
    28801 tctcatttac gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg
    28861 atctgcaccg caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa
    28921 cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat
    28981 gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg
    29041 tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa
    29101 caaaaacttt gttatggcct gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt
    29161 cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat
    29221 gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt
    29281 gcggtggcac tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg
    29341 ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc
    29401 gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa
    29461 attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt
    29521 taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt
    29581 cggtagtgag caatttatcg cccagttgcc acgccccgtt aatcacaaac accaccccgc
    29641 cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat
    29701 ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt ttcgctttta
    29761 ccacctggtc cgctgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt
    29821 ctgcgctttc aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccatgccgg
    29881 gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtgaaaat
    29941 cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca
    30001 gattcaccga cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt
    30061 cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt
    30121 ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc
    30181 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat
    30241 tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca
    30301 aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg
    30361 gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct
    30421 tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct
    30481 tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg gatctgctgt
    30541 ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg
    30601 cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca
    30661 tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta
    30721 cgcatattct atatccttcc tttcagtatt tattacggct ttcttaagta gcgtgccgtg
    30781 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
    30841 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
    30901 gatgagagaa taatacaaca cgatgatttt gcagagatta tgaagaacta taccggatga
    30961 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
    31021 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
    31081 cgcgcgtaat cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt
    31141 tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa
    31201 attcagcgtg tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga
    31261 aaccacccga taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag
    31321 cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa
    31381 cgctttgcca agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt
    31441 aaacgtacca ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca
    31501 cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg
    31561 caaggagcca cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgcccag
    31621 gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc
    31681 agcaaatagc tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt
    31741 cccttgcttc agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat
    31801 ctcgccgtga gatatcgcgc caagcaacgc gtccagcatc tccacaggtg ccgtctcatt
    31861 accgtgtatt cccgctgaaa tcaccagtgc gccttgcggt ggcgttaatg gcgtcagttc
    31921 cagcacacca tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac
    31981 cggtttttta cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct
    32041 ggaacggata aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc
    32101 ctccgcgcag caattgcggg tcggccagat cggcagcagt aaggcgatcg cggtagtaac
    32161 gatccaccca gtcattgagc gcattaaaca gcgtatcgtt catcatcacc gccggattca
    32221 ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc
    32281 cattcgccat gctttcacgt aaatcaaaga cttttagttc gctaatcggg ttgtcagcgg
    32341 caaggagttc attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca
    32401 ccaacatcat ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca
    32461 ccgcatccga cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc
    32521 gcgcacgcag gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca
    32581 gcacctggcg gttactcacg gcaatcacgt cattatgaaa aacgccctgg tcgataacgt
    32641 ccgggttttg ctgggcgaaa atcacctgtt ggggattcac ctgattcagc cttgccaccg
    32701 cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt
    32761 cttctcgccc gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat
    32821 tgtggtttgc cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatgaacgc
    32881 taaatttctc ttcgtcgtta aaaatcgctt ttaaaagtga ttcggtgacg tgcgcttcca
    32941 gcgagcggtg aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg
    33001 tatcggcaga tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggaactga
    33061 cgctggaaag ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac
    33121 cactgaatcc caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg
    33181 cctgggggaa tcccgcatcg gcaagggctt tcattttcag taagccctgt tttgccgcct
    33241 gtcgcgggtt agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc
    33301 cagcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg
    33361 caccacctca tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc
    33421 cgactccagg ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg
    33481 atggttaccg gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg
    33541 tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg
    33601 ctcgggggaa accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc
    33661 ttcctcgaaa gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg
    33721 tacgccagca acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctgattg
    33781 taataagcgc ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt
    33841 aaccacctgc tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc
    33901 atcccagttg cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc
    33961 gccctgcgct ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt
    34021 gacaaacgcc gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat
    34081 aattagcgga ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag
    34141 ctggcgatgc agctggtagc cggtattggc gctaccggta aacagcaaac cgtcgagatc
    34201 ctccagcgca ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagtacgcc
    34261 cggcggcaag ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag
    34321 ttcgctgggt ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt
    34381 cggcaaatga ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg
    34441 tcgcaggctc gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata
    34501 cgccttaatt gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca
    34561 gcgcggctta cccgtttctc tggcaataat cgcggttaat tcgcctttat tcctttccag
    34621 caatccggca aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg
    34681 cggaaacgcc gcacgggctg cgcgacaagc ctgcccgacc tgagcggcat cggcatcatt
    34741 gccttgccat aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc
    34801 cgttatccag tcaccgttaa tccataaagt catgctgttt tctcctctgc gcacaggcgc
    34861 accagacgaa cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa
    34921 atcaaacgct cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt
    34981 tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc
    35041 accagccgac ttttacgaat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg
    35101 ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg
    35161 gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag
    35221 tgggtataga tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg
    35281 cagagaaaat cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag
    35341 aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta
    35401 aacttgtcgc gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg
    35461 ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga
    35521 tcgttactga gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga
    35581 accaacgtgc cgacgcgata gttgtaccag ggatcgttca gcccaaccgc cacctcaatg
    35641 gcacaaatcc ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc
    35701 tcacttttgg gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgccgaaagc
    35761 gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc
    35821 agcgccgaga catctgatcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa
    35881 caaagtgttc gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga
    35941 cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc atcacgcctg
    36001 ctttcgccgc ttcctgagag atctgtttcg cttgcccggc gtaatcggca ttcagtacac
    36061 agccaatcag caaacctaag ccgcgaactt cactgaacaa accatagcgg tgattaatag
    36121 tattaagacg ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg
    36181 tgttgatgag ctccagcact ttgcctgcca ccgccgaggc cagcggatta ccgccatagg
    36241 tggtgccatg agtgccaacg gtcatcacgc gggcgcactc ttcggttgcc aacaacgcac
    36301 cgaccgggaa accgccgccc agcgctttgg cggtagttaa cagatcaggc gtcacgccgt
    36361 agtgcatata ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa
    36421 tcaacagcgc attgtggcgg ttacacaatt cacgcagacc ttgtaaaaac gcgttgctgg
    36481 ctggcaccac accgccttcc ccctggatgg gttcgacaat caccgcacag gtagagtcgt
    36541 caatcagcgc gctggcagag ttaatatcgt tatatgcagc atgacgaata tccgccggca
    36601 gtggcgcaaa atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc
    36661 gaccatgaaa cgcatttttg aacgccacga tgccgctctt atggctgccg tagcggtcgt
    36721 gagcgaattt acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa
    36781 agaagacgcg atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct
    36841 cgttggtgta accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac
    36901 gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat
    36961 actctttccc ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg
    37021 ccggagcgta aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca
    37081 tagcgacctc tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc
    37141 tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg
    37201 ctaactggaa atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga
    37261 atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa
    37321 atataattct ggaattgtga tcattgacga aatttactgg aaattactgc gtcattctga
    37381 cgcagcgcgc accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc
    37441 gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact
    37501 ctttatctga ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc
    37561 gcgccagacc tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc
    37621 tgcaggagac aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct
    37681 acaacgtgtt ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga
    37741 cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta
    37801 ttatggcgga aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc
    37861 agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc
    37921 tgcaaaacta cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata
    37981 tgaatatcag ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc
    38041 tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga
    38101 gctgggggtt ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat
    38161 ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc
    38221 tcgccagcca accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca
    38281 gcatggaaaa accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca
    38341 gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca
    38401 gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac
    38461 cacttctcta ttatcaagtt tgatatagga aactccacga tgaacgctga gcgtaacttt
    38521 ctgtttgcct gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc
    38581 gatctgctca ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt
    38641 tatagcctct atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata
    38701 ctggtgatcg ctggaggaat agtttttggc ccgctcttag ggacactact ctcattaatt
    38761 gccgccacgc tggcctcctc gagctcattc ctgctggcgc gctggctggg acgtgattta
    38821 ctgctgaaat acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt
    38881 aacggtattg attttcttat tctgacccgc taaatcccgt tgtttcctta caatattcaa
    38941 aattacgctt acggattaac caccatcgcc ttctggcctt atacccttat ttcggcactc
    39001 acgaccctgc ccggtattgt tatttatacc gtgatggcaa gcgatctcgc cagtgaaggc
    39061 attacgctgc gctttatttt acaactctgt ctggcgggcc tggcgctgtt tattctcgtc
    39121 cagctcgcaa aactctacgc ccgacacaaa catgtggatc tgtctgcttc gcgccgcagc
    39181 ccacttactc acccaaaaaa tgaaggatag aacgatgtcg caacattatt cagtgtcatg
    39241 gaaaaaagga ctggctgcac tctgcttact ggctgttgcc gggcttagcg gctgcgatca
    39301 aaaagagaat gcggcggcaa aagtggaata cgacggactt tcgaacagcc aaccgctgcg
    39361 cgtcgatgcc aataaccata cggtaaccat gctggtgcaa attaatggtc gtttcctcac
    39421 cgacgtcact cgtcacggta ttgtgtttaa agatggctcc aacggacata aatcgctgtt
    39481 tatggcttat gcgaccccga aagcatttta tgaagccctg aaagaggcag gtggtacgcc
    39541 gggcgaaaat atgacgatgg ataataaaga aacgactctt gtcacaggca gcaaactgga
    39601 tatttcggtc aactggcaag gggcggcaaa agcgtattcc ttcgatgaag tgattgttga
    39661 cagtaatggc aagaaactgg acatgcgctt tggcggtaat ttaacggcag cagaagagaa
    39721 gaaaacgggt tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca gcaatgcaac
    39781 atacacttat ggtgcggtgg aaaaacgcgg cgaagtgaaa ttcaaaggca atgcctcagt
    39841 tctcccggcg gataacacgc tggcaacggt tacctttaaa atcaccgaat aaagccagga
    39901 taaaggatga tgaagataca atcgcgtaaa atctggtact accgtataac cctcatcatc
    39961 ctgttgttcg ctatgctgct cgcatgggcg ctgcttcccg gcgtccatga gtttatcaat
    40021 cgcagcgttg cggcgtttgc cgccgtggac caacagggta tagaacgctt tattcagtct
    40081 tacggcgcac tggcggcagt tgtctcgttc ttgttgatga ttttacaggc cattgctgca
    40141 ccgctacctg cgtttttgat cacctttgcc aatgcgtcgc tgtttggcgc gttctggggc
    40201 ggcttgctgt cgtggaccag ttcgatggcc ggcgcggcgc tgtgcttttt tatcgccaga
    40261 gtgatgggcc gcgaagtggt ggaaaaatta accggcaaaa ccgtgcttga cagtatggac
    40321 ggctttttca ctcgctacgg caaacacacc atcctggtct gtcggttatt gccttttgtc
    40381 cctttcgatc caatcagcta tgctgccggt ctgacttcaa tacgttttcg ctcgtttttt
    40441 atcgccaccg ggcttggtca attaccggcg actattgttt attcctgggc gggcagcatg
    40501 ttaacaggcg gtactttctg gtttgtcacc ggactgttta ttctgtttgc cctgaccgtg
    40561 gtgattttta tggcgaagaa aatatggctt gagcgccaga agaggaatga ctgatgggtt
    40621 taccgccgct tagcaaaatt cctttcattt tacgtccgca ggcgtggctg catcgtcgcc
    40681 attacggcga ggtgctaagc ccgattcgct ggtgggggcg gatcccgttt atcttttatt
    40741 tggtgtcaat gtttgttggg tggctggagc gcaaacgctc accgctcgat ccggtagtgc
    40801 gatcgcttgt cagcgcgcgc attgcgcaaa tgtgcctgtg tgagttttgc gtggatatca
    40861 ccagtatgaa agtcgccgag cgtaccggca gcaacgataa actgctggca gtggctgact
    40921 ggcggcaaag cccgctcttt agcgatgaag aacggctggc gctggagtac gccgaagccg
    40981 caagcgtcac accgccaaca gtcgatgatg ccctgcgtac ccgactggct gcgcattttg
    41041 acgctcaggc gctcaccgaa ctgacggcat tgatcggcct gcaaaacctg tcggctcgtt
    41101 ttaattctgc catggacatt cccgctcagg ggctgtgccg tattccggaa aaacgttctt
    41161 aaggagggaa gatgcgccat tgtgggtggt tgctgggatt gttatcgctg ttttctttgg
    41221 caacacatgc cagtgactgg caggaaatta aaaatgaggc caaagggcaa accgtctggt
    41281 ttaacgcctg gggcggcgat accgcaatta accgctatct cgactgggtt agcggcgaga
    41341 tgaaaaccca ttacgctata aacctgaaga ttgttcgtct cgcggatgcc gcagacgcgg
    41401 tgaagcgcat tcagaccgaa gctgctgccg gacgtaaaac gggcggctcg gtggatctgc
    41461 tctgggtgaa cggcgaaaac ttccgcacct taaaagaggc caatttatta caaacgggct
    41521 gggcggagac tctgcccaac tggcgctatg tcgacacaca gctgccggtg cgggaagatt
    41581 tttcagtgcc gacacaaggt gcggaatcgc cctggggcgg cgcacaactg acgtttatcg
    41641 cccgccgcga tgttacgcca cagccaccac aaacgccgca agccttactg gagtttgcta
    41701 aagtcaatcc cggcacggtt acttatccgc gcccaccgga ctttaccggc acggcgtttc
    41761 ttgaacagtt gctgattatg ctgacgccag atcccgccgc attaaaagaa gcgccggacg
    41821 atgcgacttt cgcccgtgtc actgctccct tgtggcaata tcttgatgcg ctacatccgt
    41881 atttgtggcg cgaaggaaag gatttcccgc cttcgcccgc gcggatggat gctctgctga
    41941 aagccggcac attgcgcctg tcgctgacct ttagccccgc gcatgcgcag caaaaaatcg
    42001 ccagcggcga tttgccggca agcagttaca gttttggctt tcgcgagggg atgataggca
    42061 acgtgcattt cgtcaccatt ccagccaacg cgaatgccag tgctgcggcg aaggtagttg
    42121 ccaatttcct gctctcaccc gatgcgcaac tgcgtaaagc agatcccgtt gtctggggcg
    42181 atccttctgt tctcgatccg caaaaactgc ctgacgggca gcgcgaaata ttgcaatcaa
    42241 gaatgccgca ggatctgccg ccggtactgg ctgaaccgca cgcaggatgg gtgaatgcac
    42301 tggaacaaga atggttacgc cgttacggta cgcattaatc tttttgctgt gggcgatggt
    42361 ggcggtgatt tatgcaccgc tgatcccggc agctctcacg ctgatatcgc ctgcgttgtc
    42421 gttgacacac tggcaggcgt tatttgccga tccacagtta ccgcatgcat tactggcaac
    42481 gctggtgtcg acaaccatcg cggcggtcgg ggcattgttg attgccctgc tggtgattgt
    42541 ggcgctatgg cctgggccga aatggcagcg tatgtgcgcc cgtctgccgt ggctgctcgc
    42601 cattccccat gtggcttttg ccaccagcgc cctgctgctc tttgctgacg gagggctgct
    42661 atatgactat ttcccgtatt tcactccgcc aatggaccga tttggcatcg gtctgggcct
    42721 cacccttgca gtgaaagaaa gcgcatttct gctgtggatc ttagcggcag tattgagcga
    42781 aaaacggctg ttgcagcagg tcattgtgct ggattcgctg ggctacagcc gctggcaatg
    42841 cctgaactgg ctgctgttgc cctccgtcgc gccttcgctg gcaatggcga tgctggcgat
    42901 tgttgcctgg tcgctttcgg tcgtggatgt ggcgattatt cttgggccag gtaatccacc
    42961 gacgctggca gtaattagct ggcagtggtt aactcagggc gacgcggatc aacaaacgaa
    43021 aggcgcactc gccagtctgc tgctgatgct gttactcgcc gcctacgttt tgctgagcta
    43081 tctgctatgg cgcagctggc ggcgcactat tccccgcgta gatggcattc gcaagcctgc
    43141 cacaccttta ttaccaggca ctacgctggc gagtttttta cccttaaccg gtgtgctgtg
    43201 tgtggttctg ctggcgatcc tcgcggatca gtcgacgatc aatagtgaag cactcatcaa
    43261 cagcctgaca atggggctgg tggcgacatt catcgctttg ctcctgttac tgctgtggct
    43321 ggaatggggg ccacagcgtc gccagttgtg gctctggttg cccattctat tgcccgctct
    43381 gccactggtg gcgggccagt acacgctggc gctatggctg aacctggatg gaagctggac
    43441 ggcggtggtc tgggggcatc tgctgtgggt aatgccgtgg atgctgttta tcctgcaacc
    43501 cgcctggcag cgcattgatt cacggttaat tttgattgcg caaacactgg gctggtcgcg
    43561 ggccaaaatc ttcttttacg tgaaatgccc actcatgttg cgccctgtgc tgattgcctt
    43621 cgcggtggga ttttcagtca gtattgcgca gtatatgccg acgctgtggc tgggcgcggg
    43681 gcgttttccg acgctcacca ctgaggcggt ggcattaagc agcggcggca gcaacggtat
    43741 tctcgccgcc caggctttat ggcaactgtt gttaccgctt attatttttg ccctgaccgc
    43801 gttagtcgca aaatgggtag gttatgtcag acaaggactc cgctaatgct ctgcgtgaaa
    43861 aatgtttcgc tacgtttacc agaaagccgc ttgctgacaa acgttaactt tacggtggat
    43921 aaaggtgaca ttgtcacgtt aatggggccg tctggctgtg gaaaatccac tctgttttca
    43981 tggatgattg gtgcactggc tggacagttt tcttgtacag gtgagctatg gttcaatgag
    44041 caacggattg acatgctacc caccgcacag cgtcagattg gcattctttt ccaggatgca
    44101 ctgttatttg accagttcag cgtcgggcaa aatttactgc tggcgctacc gtcgacgctc
    44161 aaggggactg ccaggcgcaa tgccgtgaaa gacgcgcttg atcgagcggg actggctgaa
    44221 acttatcacc aggaccctgc gacattatca ggcggtcagc gagcgcgggt ggccctgctg
    44281 cgcgcccttc tcgctcagcc aaaagcgtta ctcctggatg agccattcag ccgtcttgat
    44341 gtggctctgc gcgataattt tcgccagtgg gtgttcagcg aagttcgcga actggcgatc
    44401 cccgtcgttc aggtaacgca cgatctccag gatgttcctg ctgatagttc tgttctggat
    44461 atggcgcagt ggtcagaaaa ttacaacaaa ctgcgataac gcaaagtttt tctcaatgcg
    44521 tcagttcaga atggcgcact caaaactaca atgtcgggat tttcgatgaa acgtgtttct
    44581 caaatgaccg cgctggcaat ggctttaggg ctggcttgcg cttcttcgtg ggccgctgaa
    44641 ctggcgaagc ctcttacact tgaccagctt caacaacaaa atggcaaagc gatagatact
    44701 cgccccagcg cgttttataa cggctggcca caaaccttaa atggcccttc tggtcatgaa
    44761 cctgccgcct taaacctctc tgccagctgg ctcgacaaaa tgagcaccga acagctctat
    44821 gagtggatca agcaacataa tctgaaaacc gatgctccgg tggcgctgta cggcaatgac
    44881 aaagatgtcg acgccgtcaa aacgcgtctg caaaaagcag gttttacgca tatctccatt
    44941 ctgagtgacg cgctaagcga accttcccgt ctacaaaaac tgccgcactt tgagcagctg
    45001 gtttatccgc aatggctgca cgacctgcaa caaggtaaag aggttacggc gaaacctgcc
    45061 ggtgactgga aagtcattga agcggcctgg ggcgctccta agctttacct tatcagccat
    45121 attcctggcg ctgactacat cgataccaac gaagtggaaa gcgaaccgct gtggaacaaa
    45181 gtttctgatg aacaactaaa agcgatgctg gcaaaacacg gcattcgcca tgacaccacg
    45241 gtcattctgt acgggcgtga cgtatacgct ggagcgcgtg tggcgcaaat tatgctctac
    45301 gcaggcgtga aagatgtgcg tctgctggat ggcggctggc aaacctggtc cgacgcggga
    45361 ctgcctgttg agcgaggaac gccaccgaaa gtgaaagcgg aaccggattt cggcgtgaag
    45421 atcccggcac aaccgcagtt gatgctcgat atggaacagg cccgtggact gctgcatcgc
    45481 caggatgcat cgctggtgag cattcgttcg tggccagaat ttatcggtac gaccagcggt
    45541 tacagctata ttaaaccaaa aggtgaaata gccggagcac gttggggaca cgctggtagc
    45601 gactcgacgc atatggaaga ttttcataac ccggatggca ccatgcgcag cgccgatgat
    45661 attaccgcta tgtggaaagc gtggaatatc aaaccagagc agcaagtttc attctactgc
    45721 ggcaccggct ggcgcgcgtc cgaaaccttt atgtacgcac gcgccatggg ttggaagaat
    45781 gtttccgtgt atgacggcgg ctggtacgaa tggagcagcg atccaaaaaa tccggtagca
    45841 accggtgaac gcggcccgga cagtagcaaa taacattgca attactgacg ctggagcgac
    45901 ttcagcgtca gatacccact ccacacccgc gtaaaggtcg tcatccagca caacgcaccg
    45961 aagatccacg caaaccacgc aaaccatgca gggaataaac atcccagcac aaacagtaag
    46021 atcgtttctg tgccttcggt taatcccccc agatagtaaa acgacttatg tgcataaccg
    46081 gggttatcaa tctgatgttt tgctgccagt gcagcaaagg cgagaaaact gctgcccgtg
    46141 ccgataaacg caaacaacaa ccagccgcct gccagcgcat tttgctccgg tgcagcaaga
    46201 ataaagccaa acggcaccag cgcgtaaaag agaaaatcga gagaaatatc aagaaagccg
    46261 cccgcgtcgg taagctctct gcgccgcgcc agcgcaccgt ccagaccatc aagcagcctg
    46321 ttcaacaaaa tgacgactag cgccgccaga taccagccca gcgccagaaa cggcagagcc
    46381 agcacgccga tggcaaatcc cactaatgtt aaaccatctg gcgtaatgcc cggtttatca
    46441 agaacccgca cgcactgatg caacaacggt ttaatccggg gatgaagatg gcggtctagc
    46501 acaggcactc cttaaatata aagcctttct gattgagcaa cagtgcggat attatggcat
    46561 ttttcgctta tctgcccgtg tgtaatttat gaaaatgatt gaagttgttg ccgccatcat
    46621 tgaacgtgat ggcaaaattt tactcgcgca acgccccgcc cagagcgatc aggcgggatt
    46681 atgggagttt gccggtggta aagtcgagct ggatgaaagc cagcagcagg cactggtgcg
    46741 tgagttaaac gaagaattgg gcatcgaagc aactgtgggt gagtatgttg ccagccatca
    46801 gcgagaagtt tcgggccgga ttatccatct tcatgcctgg cacgtacccg acttccacgg
    46861 gacgttacag gcacatgaac atcaggcgct ggtctggtgc tcacctgaag aggcgctgca
    46921 atatccgctg gcccctgctg acattccact attagaggcg tttatggctt tacgcgccgc
    46981 cagagcagcg gattagtgct aagggttttg tcatcccgct ggcattgcag cagtattcct
    47041 tcggctttaa ttaccgcccc ttcagaataa ttttgatcct gataaacgca gcactgagta
    47101 cagggctgcg ctgactgccc gcctgaactg aacacttctg gcggtacgtt tacctccacg
    47161 tccggacgat aatgcgggtt agccagtgca attaatggaa atgctaatac tacggcgaac
    47221 aatgctcgac tcacagggaa ctccttaacg ttattgtctc tgctactgat aacggtagcc
    47281 gggtggcaaa actttagcgt ctgagttatc gcatttggtt atgagattac tctcgttatt
    47341 aatttgcttt cctgggtcat ttttttcttg cttaccgtca cattcttgat ggtatagtcg
    47401 aaaactgcaa aagcacatca cataaacaac ataagcacaa tcgtattaat atataagggt
    47461 tttatatcta tggatcagac atattctctg gagtcattcc tcaaccatgt ccaaaagcgc
    47521 gacccgaatc aaaccgagtt cgcgcaagcc gttcgtgaag taatgaccac actctggcct
    47581 tttcttgaac aaaatccaaa atatcgccag atgtcattac tggagcgtct ggttgaaccg
    47641 gagcgcgtga tccagtttcg cgtggtatgg gttgatgatc gcaatcaggt acaggtcaac
    47701 cgtgcatggc gtgtgcagtt cagctctgcc atcggcccgt acaaaggcgg tatgcgcttc
    47761 catccgtcag ttaacctctc cattctcaaa ttcctcggct tcgaacaaac cttcaaaaat
    47821 gccctgacta ccctgccgat gggcggtggt aaaggcggta gcgattttga cccgaaaggt
    47881 aaaagcgaag gcgaagtgat gcgtttctgc caggcgctga tgaccgagct ttatcgtcat
    47941 ctgggcgcgg ataccgacgt tccggcaggt gatatcggcg ttggtggtcg tgaagtcggc
    48001 tttatggcgg ggatgatgaa aaagctctcc aacaataccg cctgcgtctt caccggtaag
    48061 ggcctttcat ttggcggtag tcttattcgc ccggaagcta ccggctacgg tctggtttat
    48121 ttcacagaag caatgttaaa acgccacggt atgggttttg aagggatgcg cgtttccgtt
    48181 tctggctccg gcaacgtcgc ccagtacgct atcgaaaaag cgatggaatt tggtgctcgt
    48241 gtgatcactg cgtcagactc cagcggcact gtagttgatg aaagcggatt cacgaaagag
    48301 aaactggcac gtcttatcga aatcaaagcc agccgcgatg gtcgagtggc agattacgcc
    48361 aaagaatttg gtctggtcta tctcgaaggc caacagccgt ggtctgtacc ggttgatatc
    48421 gccctgcctt gcgccaccca gaacgaactg gatgttgacg ccgcgcatca gcttatcgcc
    48481 aatggcgtta aagccgtcgc tgaaggggca aatatgccga ccaccatcga agcgactgaa
    48541 ctgttccagc aggcaggcgt actgtttgca ccgggtaaag cggctaatgc tggcggtgta
    48601 gcaacgtcgg gcctggaaat ggcacaaaac gctgcgcgcc tgggctggaa agccgagaaa
    48661 gttgacgcac gtttgcatca catcatgctg gatatccacc atgcctgtgt tgagcatggt
    48721 ggtgaaggtg agcaaaccaa ctacgtgcag ggcgcgaaca ttgccggttt tgtgaaggtt
    48781 gccgatgcga tgctggcgca gggtgtgatt taagttgtaa atgcctgatg gcgctacgct
    48841 tatcaggcat acaaatgggc acaattcatt gcagttacgc tctaatgtag gccgggcaag
    48901 cgcagcgccc ccggcaaaat ttcaggcgtt tatgagtatt taacggatga tgctccccag
    48961 ggaacatttc ttatgggcca acggcatttc ttactgtagt gctcccaaaa ctgcttgtcg
    49021 taacgataac acgcttcaag ttcagcatcc gttaactttc tgcgatagca gcagatatgc
    49081 ctgtaaagaa atcccattag actatttttt tgataatctt cttcgctttc gaacaactcg
    49141 tgcgcctttc gagaagcaag cattatataa tgccaggcca gttcttcttc aattgtcccg
    49201 ttttgaaaag ctgtgcttga tatcgagatc atccatgata attccgccgc ccatattagc
    49261 ttcgccgagg atttaccgga gcgattatta gcgcaatcag agatatagtc tgagggaaaa
    49321 acagcaaatt tattcaacaa ggcgataacc tgctctgggg cttcctccat gtttgcttta
    49381 aaggtattgg ctccatggtc gccagaaaga aaatgctcca ttaaggcaca ataactttcg
    49441 ctatcttcga taccccattg atcctctaaa gactcgcgtc ttttacttat gatatcgatc
    49501 gagtcaaaag gaagcacatg atattggaag gtatctttgc caggttcagg ctttcgcggc
    49561 cagaactcca gcgtttcaga ccattgctta tgatagaatc gataaggtgc gatcaattgt
    49621 agcgcctgta acttctcgat actgagcggc tcaatacctt tagcctgata ataatgcagt
    49681 tgttcttttt ttgctttaaa accggcccga acaataagcc ccatcataat taatagataa
    49741 agaaaagagc atcccgcagt aatcaggcct ctttcattca aaccgttgga tgttatcgct
    49801 gcgaatacaa acattacagc gacaacacat gttaaatacc cccccccact tacaaagcag
    49861 catggcctta ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt
    49921 aaaagaatac ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc
    49981 tgttattaca tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt
    50041 ttcttgatcc gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt
    50101 acgctatcgc cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac
    50161 gcggtgcagc ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa
    50221 actgccgcac cggcgtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg
    50281 gctgcataaa gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc
    50341 agtgcgcggt catgtccggt cgcgtagcca tttctggcag cgaatggaat aacgcttttc
    50401 cggcgtcggt ggagtggata tagcgccctt ttttggtgag gaaaccacgc ttgaacaaca
    50461 gttcaataat cccggcacgc gttgcttctg tccccagacc atcggtcgca cgaaggatct
    50521 ttttcagatc tttgtcctgc acaaagcgcg caatcccggt catcgccgaa agcagtgttg
    50581 catcggtaaa atggcgcggc ggctgggttt gccgctctac cacttcacct ttttcacaca
    50641 gcaactcatc gcctttcgcc accacaggca atggcgtgcc gtcgttttct tcatcgcgct
    50701 ctttgctgcc taacagcgcg cgccaacctg cttcagcaag aaaacgcgct ttagcgacaa
    50761 atttgccttt ggcaatgtcc agttcgataa cacacttgcg gaacactgca tccgggcaga
    50821 actgcatcag atactgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt
    50881 tgatcgcaga actccttgcg gtcggaatga tggcgtggtg cgcatcgacc tttttgtcat
    50941 cccaacagcg gttgcgtata tctggatcta ccactggctg cggcaacaga tctggtgcat
    51001 gaacgctgat ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc
    51061 gacaatcgga acgcggataa gtgattaact tgtgtgtttc gtacagcttc tggcagatat
    51121 caagcacgtt ctgcgcgctc agaccaaagc gttttgccgc ttcaatctgc aacgccgaaa
    51181 gcgaaaacgg cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgacaatag
    51241 ccggttgacc actaatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc
    51301 cttcttcatc ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct
    51361 catcggcagg tgtcacgata tgcgctttga cttcaaagaa gtctttcgcc acgaagtttt
    51421 caatctcttc atcgcggcgc accaccagcc cgagcacggg tgtctgcacg cgtcccacgg
    51481 aaagtacgcc ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatattga
    51541 tgccgtacag ccagtcggca cgcgctcgcg ccagcgcaga aacgcacaat ggcacaaact
    51601 cactgttgga acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta
    51661 tcaagcaacg ctgcacctgc tggcgctttt ccggtgccag ttgcagatag tccagcactt
    51721 catccaccag caattgccct tcacgatccg ggtccccggc gtgaacgatt tcgctggctt
    51781 catgcaggaa gcgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt
    51841 gccacttttc cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt
    51901 aggcgtctgg ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat
    51961 taccgcactc gataaagcca tcgcctttac ggtgcggttt gggcaggaca tcagcaatgg
    52021 cgcgcgccag actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc
    52081 atggcacgac cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac
    52141 tcggcggctg tagttttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg
    52201 ccagaagttt gcggatcgca cagcagatcg cgcacttcac gcggcatttc acccatcaga
    52261 tgaccgtagc tggcaaagtt acgttcggtg ccgccaggca ctgcgcccaa cttaatgtac
    52321 tcttcaaccc ccggcaattt tgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg
    52381 ccctgacaca tttcgttcaa atggcccagc agaccaaaac cagtaacgtc ggtcatcgct
    52441 tttacgcctt cgatgttggc aaaggacgca cctgcgatgt tcatccggca catcacttcc
    52501 gtcgccagtc cctgatgttc tggtttcaac agtgattttt tctcagccgt ggtaagaacg
    52561 ccgatcccca gcggtttcgt caggaacagt ttgcatccgg cttgtgcggt actgtttttc
    52621 ttcacccgct cggtcggtac gatccccgtt accgccagac caaaaatcgg ctccggcgca
    52681 tcgatggagt gacccccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct
    52741 tcggttactt cgcgggcaat ttctggggaa agtttgttaa tcggccagcc gaggatcgca
    52801 atcgccataa tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca
    52861 atgcggccaa aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata
    52921 acgctggtgc cattgcccag atcgtacacc gccgcatcgt cgcgggtctc attacccaca
    52981 agcaaattcg gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt
    53041 ggggaaattt tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc
    53101 tcgctcatgg acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt
    53161 gataagtgag agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca
    53221 attatcagaa ataagttaca aacggcgtcg ggtccgggac gttaatcgac gtagatgctt
    53281 tcaactgcgg cgtgccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga
    53341 atgcttcacg cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaatc
    53401 cttgggcaac ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt
    53461 cccatcgcgg gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg
    53521 cgcggaacgg cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg
    53581 ccccctgttc cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat
    53641 gccacggttg catggactta tggtccggcg cacgcatacc cgcacgcagg atgttttgca
    53701 gttgttcacc cgttggcgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata
    53761 gttcgagtgc atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga
    53821 gagtggattt tgttacagca cagtccgcaa ttcctgctga caagtgccgg ttgggtcatt
    53881 acgataacca catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc
    53941 gaaccctttg gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg
    54001 tccgtgaaat ggtacttaac ctgttcttta ttttcctcgt actggttggt gtggggatct
    54061 ggatgcaggt cagtggtggt gattcgaaag aaacggccag tcgtggcgca ctgctgctgg
    54121 acatttctgg tgtgatcgtc gataaacccg acagttctca gcggtttagt aaattaagcc
    54181 gccagctgct tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca
    54241 acaccattcg ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg gatctgaaaa
    54301 acttcgcagg cggcgaccag ccgtctatgc agtacatcgg caaagccctg aaagagtttc
    54361 gtgacagcgg gaaaccggtt tatgccgttg gcgagaacta cagccagggg caatattatc
    54421 tcgccagttt cgccaataaa atttggctgt caccgcaagg cgtggttgat ctgcacggtt
    54481 ttgctaccaa cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg
    54541 tgttccgcgt gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gatatgtcac
    54601 cggcagcccg cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata
    54661 ctgttgccgc taaccggcag atccctgctc agcaggtatt ccctggcgcg caagggttgc
    54721 ttgagggttt aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg
    54781 tcgatgcact ggcatcgagt gccgaaatcg aaaaagctct gaccaaagag ttcggctgga
    54841 gtaagactga taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg
    54901 cagataccgg tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg
    54961 aaactcaggg gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg
    55021 acccgaaagt gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt
    55081 ctgaagtgat tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat
    55141 cgatgggcgg catggcggca tctggtggtt actggatttc cacgccagct aattacattg
    55201 tggctaaccc cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag
    55261 aaaatagtct ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcgg
    55321 atgtttctat caccagggca ctgccgccgg aagcgcagca gatgatgcaa ttaagcattg
    55381 agaatggcta taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc
    55441 aaattgataa aatcgcccag ggccacgtct ggaccggtca ggatgcaaaa gctaacgggc
    55501 tggtcgatag tctcggggat ttcgatgatg cggttgccaa agcagcagag ctggcaaaag
    55561 tgaaacagtg gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg
    55621 acaacatgtc tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg
    55681 caccgcttgc ctcggtagcc tctaccgtta aaagtgaaag cgacaagctg gccgcgttta
    55741 acgacccaca aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taagtcttgt
    55801 actgagtggc cgacagatcg tcggccacat tattttttac ctcgacgaat cctcttcccg
    55861 ctgtctcgac ccatatttcc ttatccgcat agtatcaggt gcgctcctcc tgcctcacgt
    55921 atatactttt gctctttcga tatcattcat atcaatatca tgcaaaagaa atcaatttac
    55981 gtcgcctaca cgggcgggac catcgggatg cagcgttccg agcagggtta tattccggtg
    56041 tcaggtcatc tacaacgcca actggcgctg atgccggaat tccatcgccc ggagatgcca
    56101 gatttcacca ttcatgaata tacgccgttg atggattctt ctgatatgac gccggaagac
    56161 tggcagcata ttgctgaaga tattaaagcg cactatgacg actatgatgg ttttgtcatt
    56221 ctacacggta ccgacacgat ggcgtatacc gcctctgcgc tgtcgttcat gctcgagaat
    56281 ctcggtaaac cggtcattgt gacagggtca caaatcccgc tggctgagtt acgctctgac
    56341 ggacaaatta atctgctgaa tgcgttgtac gttgcggcga attatccgat caacgaagta
    56401 acgctctttt tcaataaccg attgtatcgc ggcaaccgca ctaccaaagc ccatgccgat
    56461 ggttttgatg cgtttgcctc tccaaacctt cctccgttac tggaagctgg tatccatatt
    56521 cgtcgtttga atacgccacc cgccccgcac ggtgaagggg aattaatcgt tcatccaatc
    56581 acgccacaac caattggcgt agtgacgatt tatccgggga tttctgctga tgtcgtgcgc
    56641 aattttctgc gccaaccggt gaaagcattg attctgcgct cctatggcgt gggtaatgcg
    56701 ccacaaaaca aagccttcct gcaggaatta caagaagcca gcgatcgcgg tattgtggtg
    56761 gttaacctga cacaatgtat gtccggtaaa gtgaacatgg gtggttatgc caccggtaac
    56821 gccctcgccc atgccggcgt aattggcggt gcagatatga ctgtagaagc tacgctaacc
    56881 aaactgcatt acctgctgag ccaggaactg gatactgaaa ccattcgcaa ggccatgagc
    56941 caaaacctgc gcggcgaact gacgccggat gattaaggag actgcaatgc cccctcgcgc
    57001 cctgttactg gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg ccgtgccgga
    57061 aggtgacagt acggtggatg tcgctaaccg ccttattgac tggtgccagt cgcgcggtga
    57121 agcggttatc gccagtcagg actggcaccc ggcgaatcac ggcagttttg ccagtcagca
    57181 ctgtgtagag ccttatatgc caggtcaact cgacggtttg ccacaaacct tctggccaga
    57241 tcactgtgtg cagaacagtg aaggcgcaca attacatccg ttactgaacc aaaaagcgat
    57301 cgcagcggtg ttccataaag gcgaaaatcc tttagttgac agttacagtg ccttttttga
    57361 taacggccgt cggcagaaaa cctctctcga tgactggtta cgcgatcatg aaatcgatga
    57421 attgatcgtt atgggcctgg ctactgacta ttgcgtgaag tttaccgtgc tggacgcgtt
    57481 acagttaggt tataaagtaa acgtgattac cgatggttgt cgtggcgtga atatccagcc
    57541 ccaggacagt gcgcacgcgt ttatggagat gtcagcagct ggggcaacgc tatatacgct
    57601 ggcagactgg gaagagacgc aggggtaata ttagttgaat tcatctctgg cctacatttc
    57661 ctcaatgaca ttgtaggcca gataatactt gtcagtgttg catttaacct acttgcagct
    57721 aaaacacgac gtcctaacaa gtatatttgc cctcccttaa taacaggctt aaatattaga
    57781 taactcattc acttaaacat agcaataggc tttgctgaga taccaaaatc ttcttttaac
    57841 tgctgtttgc tcttcatgac catctgacca tttgtgtcaa ttgtcatatg ctgtgcttcg
    57901 gtattatggc gggcttgcca cagcatcacc agttgcaggc aattctcttt ttgttcctcg
    57961 gtcaacgcaa cgccatcagg ccatttcccc agttcaacgg cggtcgacaa acgctggtat
    58021 acctcaggcg tcatgctgtt gataatgtca tcaagattca taatttttcc gctcctgtgg
    58081 aataagttgc tgaatcgttt tttcaaccgt tgatttcttc gccgttttcg ccatcggtaa
    58141 agcgtaaaga ggcagagtta acacaataac gttcgcccgt tggctgcggc ccgtcaggga
    58201 agacatgccc cagatgggca tcacagttac cgcaacgaat ttctatgcgc tgcattccat
    58261 gtgacaagtc tttgatataa cgaatggatt cttcactcac cggttcgtag aaactgggcc
    58321 aaccacagcc ggaatcatac ttggtttggg aatgaaacag cggggcatcg cagatcaaac
    58381 agtgatatac gccgtcacgc ttgttatgca gtaaacgacc cgtaaatggc ggctctgtcc
    58441 catgattctg cgtcacgtaa aactgcatct cggacaaatt ttttttcagt tcttctgccg
    58501 aaggtttatt agccatttgc tcacatctca ctttaatcgt gctcacatta cgtgactgat
    58561 tctaacaaaa cattaacacc aactggcaaa attttgtcct aaacttgatc tcgacgaaat
    58621 ggctgcacct aaatcgtgat gaaaatcaca tttttatcgt aattgccctt taaaattcgg
    58681 ggcgccgacc ccatgtggtc tcaagcccaa aggaagagtg aggcgagtca gtcgcgtaat
    58741 gcttaggcac aggattgatt tgtcgcaatg attgacacga ttccgcttga cgctgcgtaa
    58801 ggtttttgta attttacagg caacctttta ttcactaaca aatagctggt ggaatatatg
    58861 actatcaaag taggtatcaa cggttttggc cgtatcggtc gcattgtttt ccgtgctgct
    58921 cagaaacgtt ctgacatcga gatcgttgca atcaacgacc tgttagacgc tgattacatg
    58981 gcatacatgc tgaaatatga ctccactcac ggccgtttcg acggtaccgt tgaagtgaaa
    59041 gacggtcatc tgatcgttaa cggtaaaaaa atccgtgtta ccgctgaacg tgatccggct
    59101 aacctgaaat gggacgaagt tggtgttgac gttgtcgctg aagcaactgg tctgttcctg
    59161 actgacgaaa ctgctcgtaa acacatcacc gctggtgcga agaaagtggt tatgactggt
    59221 ccgtctaaag acaacactcc gatgttcgtt aaaggcgcta acttcgacaa atatgctggc
    59281 caggacatcg tttccaacgc ttcctgcacc accaactgcc tggctccgct ggctaaagtt
    59341 atcaacgata acttcggcat catcgaaggt ctgatgacca ccgttcacgc tactaccgct
    59401 actcagaaaa ccgttgatgg cccgtctcac aaagactggc gcggcggccg cggcgcttcc
    59461 cagaacatca tcccgtcctc taccggtgct gctaaagctg taggtaaagt actgccagaa
    59521 ctgaatggca aactgactgg tatggcgttc cgcgttccga ccccgaacgt atccgtagtt
    59581 gacctgaccg ttcgtctgga aaaagctgca acttacgagc agatcaaagc tgccgttaaa
    59641 gctgctgctg aaggcgaaat gaaaggcgtt ctgggctaca ccgaagatga cgtagtatct
    59701 accgatttca acggcgaagt ttgcacttcc gtgttcgatg ctaaagctgg tatcgctctg
    59761 aacgacaact tcgtgaaact ggtatcctgg tacgacaacg aaaccggtta ctccaacaaa
    59821 gttctggacc tgatcgctca catctccaaa taagttgaga tgacactgtg atctaaaaag
    59881 agcgacttcg gtcgctcttt tttttacctg ataaaatgaa gttaaaggac tgcgtcatga
    59941 ttaagaaaat ttttgccctt ccggtcatcg aacaaatctc ccctgtcctc tcccgtcgta
    60001 aactggatga actggacctc attgtggtcg atcatcccca ggtaaaagcc tcttttgcat
    60061 tacagggcgc acaccttctc tcgtggaaac ctgcgggtga agaagaagtt ctgtggttga
    60121 gcaacaacac accgttcaaa aatggcgtcg ctattcgcgg tggcgtaccg gtttgctggc
    60181 cgtggtttgg tccggcggca caacaaggtc tgcctgcgca cggttttgcc cgcaacctgc
    60241 cgtggacgct gaaatcacat cgtgaagatg ctgatggcgt agcgctgact tttgaattga
    60301 cgcaaagcga agagacgaaa aaattctggc cgcacgactt tacgctgtta gcgcatttcc
    60361 gcgtgggtaa aacctgtgaa atcgatcttg aatcacatgg cgaatttgaa accacctctg
    60421 ccctgcatac ctactttaac gtgggtgata tcgctaaggt aagcgtcagt gggctgggcg
    60481 atcgcttcat tgataaagta aatgacgcga aagaagatgt actgaccgac ggtattcaga
    60541 ccttccctga ccgtaccgat cgcgtgtatc tgaatccgca agattgcagc gtgattaatg
    60601 atgaagcgct gaatcgtatt atcgccgtag gccaccagca tcatctgaac gttgtcggct
    60661 ggaacccggg accggcgctt tcagttagca tgggcgacat gccggatgat ggctacaaaa
    60721 catttgtttg tgtagaaacg gcttacgctt cagaaacgca aaaagtgacc aaagagaaac
    60781 ctgcacatct ggcgcaatcc attcgcgttg cgaaacgtta atttgcgtta atgttgtgtg
    60841 ccgggtgcga tgcatccggc acgtatcaac atcacaccat atccagcgca gtttttcctt
    60901 ttggtgccgg atacgcttta tccagcatga ttaattccgc tgacgaaagt tcgacctcaa
    60961 gcacagccgc attctgttgg acatgggcaa tcgtggccgc ttttggaatc gccatcacac
    61021 cctgatggct gattacccac gccaacaata cttgtgccgc gctgatattg tgagcatgtg
    61081 caatttcgtt gactaccgcg ttttttaaca gtccattgcg caaccgcccg gcctgggcta
    61141 atggactgta agccattacc ggcatctgct gttgctggca ccaggggagt agatcgtact
    61201 caatccctcg tgaaccgaga tggtaaagca cctgattagt ggcacactga tttccccccg
    61261 gcagctgcca gagttcctgc atatcagcat aatcaaggtt agaaacgccc cagcggcgga
    61321 tttttccctg ggcgatcaat ttttccatcg ctgccacagt ctcttcaaaa gcgaaactgc
    61381 cagaccagtg taataagtag agatcgagat aatcagtatt gagacggcgt aaactggctt
    61441 cgcatgcatt tatcgctttt tgcccgccag cattccacgg atagacttta gagacgagaa
    61501 agacgttatc gcgcagatcg gttaatgctt ccccaaccac cttttcggca ccgccatcgg
    61561 catacatttc ggcggtatca atgagggtta aaccgagctc aatgcccgcg cgtagtgcag
    61621 caacttctgt tttgcgctga ctggcatctt cgcccatata ccatgttccc tgccctatgg
    61681 ctggcagtga gacatcgcca ctaaattgaa tcattttttg ttgcattgtt tcctcccagg
    61741 tattgcacca ccgtaatgca aaacagggcg tgacgccctg tttttatgca caaaatgccc
    61801 tggaaagatg cattatcaga atttgtaggt gatcccggta gaaatcaggc cagtccagga
    61861 tttatccacc atcgggctgt cagtaacttc atcagacaga cgggtatagc gcgcggtacc
    61921 gtaaacactc cagtcgccga ggaagttgta gctggcgctc agctccaggt aagggctcca
    61981 gctgtcgttc gggttatagc cacgcagacc gctgcgagcg gactctttgc gcgatacgcc
    62041 ataatagtat tcgttctggt tttcgctgtt ccactgcaca ccaatacccg gagtcacggt
    62101 caggccaccg ttggtgtaac gatacaacca ggccatatcc cagacaatgc cgttgctgtt
    62161 atccagggta tcgccagcca gggtggtacg caggtaaccg tactgggtaa agtgagcata
    62221 agacagacca gccatcatgg tgctcttacg gtcatccagg tgacgcattt ggtgatcgcc
    62281 actgtctttc gctttgaagt aaagcggcga ccagtaagcg gtaattgaaa gtttatccgt
    62341 tgcgtcattc cacaggtagt aaccaccacc taagccacgg aaccagaagt tatcgccttc
    62401 atagttgatt accggtactg ggtaaacatc ggtatcgtaa tctttatatg ggtgctcaac
    62461 gacacctacg cctgcgccca gggaaaattt accttcagcg tgcgctacgc ctgcagacgt
    62521 tgcgataagc actccaagtg ccagaagttt gagtttggtc acaattaatc attccttaaa
    62581 caaatgttta gcgggcgaca aagtttaccc gtcaatacat cgaaacccaa cctttttacg
    62641 ttttcatttt ttaaagtaac tgtttaattt tcctgacgcg gatgacaccg cgcttacagc
    62701 caaatgaatt tagcgttact ggctaccctg gtctttacat taattatgca aaatttatgg
    62761 atgagttgtt gatatgccat tgaaattaag aaagccgtgc aggcaagttt tccatttgcc
    62821 atctacgctt aattttgaag gtgtatcacc gggcacgttg ttctcatcgt cgataaaatg
    62881 gcatgagagt tgctgtgttt tagcaagaga cgtcgttcag tttacctctt ccgggagcct
    62941 ctactattca tatgaacggc tcttaacctg tgctaaaaaa cgaaaggacg gcataccatg
    63001 aatatattcg atcactatcg ccagcgatat gaagctgcca aggacgaaga gttcacactg
    63061 caggagtttc ttaccacttg tcggcaagat cgcagtgctt atgccaacgc ggctgagcgg
    63121 ctattgatgg ctatcggtga gcctgtcatg gtcgatacag cccaggaacc cagactttct
    63181 cgactctttt ctaaccgggt cattgcacgt tatccggcgt ttgaagagtt ttacggcatg
    63241 gaagacgcga ttgaacagat tgtctcttat ctgaaacacg cggctcaggg gctggaagag
    63301 aagaaacaaa tcctgtatct gctggggcct gtgggtgggg gtaaatcatc gcttgctgag
    63361 cgactgaaat cattaatgca gctcgtaccg atttatgtat tgagcgccaa cggtgagcgt
    63421 agcccggtca acgatcatcc gttctgtctt ttcaatccgc aggaagatgc tcagattctg
    63481 gaaaaagagt atggcattcc tcgccgttat ctcggcacca tcatgtcgcc atgggcggca
    63541 aaacgcctgc atgaatttgg tggcgatatc actaagttcc gggtagtgaa ggtctggccg
    63601 tcaattctgc aacaaattgc tatcgccaaa acggaacccg gtgatgagaa caaccaggac
    63661 atctccgcgc tggttgggaa agtcgatatt cgtaaactcg aacactacgc gcagaatgac
    63721 ccggacgcct acggctattc cggtgcgctg tgccgcgcca accaagggat catggagttc
    63781 gttgagatgt ttaaagcacc gataaaagtg ctgcatccgc tgttaaccgc cactcaggaa
    63841 ggtaactaca acgggacgga aggtatctcc gccctgccgt tcaacgggat tatcctcgcc
    63901 cactcgaacg aatccgaatg ggtcactttc cgtaataaca aaaacaacga agcctttctc
    63961 gaccgtgttt acatcgtgaa ggtgccgtat tgcttgcgca tttccgaaga gatcaaaatc
    64021 tacgagaaat tgcttaatca cagtgaattg actcacgccc catgcgcccc tggcacgctc
    64081 gaaacactgt cacgtttttc cattctttcg cgcctgaaag agccagaaaa ctccagcatt
    64141 tattcaaaga tgcgggttta tgatggcgaa agtctgaaag acaccgatcc caaagccaag
    64201 tcgtatcagg aatatcgtga ctacgccggt gtcgatgaag ggatgaacgg tctgtcgacg
    64261 cgttttgcgt ttaagatcct ctcccgcgtg ttcaacttcg atcatgtaga agtggcagca
    64321 aacccggtcc atctgttcta cgtcctggaa cagcagatcg agcgcgagca gttcccacaa
    64381 gagcaggcag aacgctatct ggagttcctg aaaggttatc tgatcccgaa atatgccgag
    64441 tttatcggca aagagatcca gacggcctac cttgaatcct attccgaata tgggcaaaac
    64501 attttcgacc gttatgttac ctacgcggat ttctggattc aggatcagga gtatcgcgat
    64561 ccggataccg ggcagctgtt tgaccgcgag tctcttaacg ccgagctgga gaaaattgag
    64621 aaaccggcgg ggatcagtaa tccaaaagat ttccgcaacg agattgttaa cttcgtactg
    64681 cgcgccagag cgaataacag cggacgaaat ccgaactgga ccagctatga aaaactgcgc
    64741 acggttatcg agaagaaaat gttctccaat accgaggagc tgttgccggt tatctcgttt
    64801 aacgccaaaa cgtcaaccga cgagcagaag aaacacgacg actttgtcga ccgtatgatg
    64861 gaaaaaggct acacccgtaa acaggtgcgt ttactgtgcg aatggtattt gcgcgtacgt
    64921 aaatcgtctt aacaaccctg gcccggtaca catgctaccg ggcctacaac gacagcgaac
    64981 cgtgggccag agaaatggca acacaggcgt agcatacagt tggcaaatgt agtacggggg
    65041 gcatatgacc tggtttattg accggcgtct gaacggcaaa aacaaaagca tggtgaatcg
    65101 ccagcgtttt ttacgccgtt ataaagcgca aattaaacag tcgatctccg aggccattaa
    65161 taagcgttcg gtgactgacg tcgacagcgg cgaatccgta tccattccca cggaagatat
    65221 tagcgaaccg atgtttcatc aggggcgtgg cggtctgcgc caccgcgtgc atccgggcaa
    65281 tgaccatttc gtccagaacg accgaattga acgtccccag ggtggcggcg gaggttccgg
    65341 cagtggacag ggccaggcca gccaggatgg tgaaggtcag gatgaatttg tctttcagat
    65401 ttcgaaagat gagtatcttg atctgctctt tgaagatttg gctttaccga atctgaaaca
    65461 aaaccaacaa cgccagctga ccgaatataa aacgcatcgg gcgggttata ccgctaacgg
    65521 cgttccggcc aatatcagcg ttgtacgttc cttgcagaac tcactggcgc gtcgcacagc
    65581 tatgacggcg ggcaagcgac gggaacttca tgcgctggaa gagaatttgg ccatcatcag
    65641 caacagtgaa cctgcgcaac tgctggaaga ggaacgtctg cgcaaagaaa ttgcagaatt
    65701 acgtgccaaa attgaacgcg tccctttcat tgacaccttc gatttacgtt acaagaacta
    65761 cgagaagcgg cccgatccct ccagccaggc agtgatgttt tgcctgatgg acgtttccgg
    65821 ttcaatggat caatccacta aagatatggc taagcgtttt tatattctgc tgtatctgtt
    65881 cctcagcaga acgtataaga acgtggaagt cgtatacatc cgccatcata cccaggcgaa
    65941 agaagtcgat gaacatgagt ttttctactc gcaggaaaca ggcggcacca ttgtttccag
    66001 cgccctgaaa ctgatggatg aggtagtgaa agagcgttat aacccggcac agtggaatat
    66061 ttacgctgca caagcatcgg acggcgataa ctgggccgat gactctccgc tttgccatga
    66121 aatcctggcg aaaaaattat tacctgttgt tcgttattac agctatatcg aaattacccg
    66181 tcgtgcacat cagacattgt ggcgagaata tgagcatctg caatctactt tcgacaactt
    66241 tgcgatgcag cacatccgcg accaggatga tatttatccg gtattccgtg aactgtttca
    66301 taaacaaaat gcaacagcta aagactaaaa ctatcagccg ggtcattatc gcctggctga
    66361 tttttagctt actgtaaatt atctcattta ttacatacat tagcttacaa tcgctttaaa
    66421 tatgacagca taacctttac ataatttagt tccagaaaac aatcattcgg aaaaatgatt
    66481 cagtcaacac gtatttccat ggggttattc tttaaatatt ttttatcgtt aacgaaaatt
    66541 gatcctggtc aaaactatat atctctgcca tcaataaaat ccagcactca cattgctctc
    66601 ctttttatgg tttctatggg tacacaaaaa ttaaaagctc aaagcttttt tattttcagt
    66661 ttattgctga cgttaatttt attttgcatc accacattat ataacgaaaa cacgaatgta
    66721 aaactcatcc cacagatgaa ttatctgatg gtcgttgtgg ctttgttttt ccttaacgcc
    66781 atcatttttc ttttcatgtt aatgaaatat ttcactaaca aacaaattct accaacactc
    66841 attttaagtc ttgcattttt aagtggcctt atctatttag ttgaaactat tgttgttatc
    66901 cataaaccta tcagcgacag tacactgatc cagacaaagt cgaatgatgt ttctattttc
    66961 tatattttcc gccaactcag ttttatttgt ttaacctcgc tggcgctctt ttgttatggt
    67021 aaagacaaca tccttgacaa caataagaaa aaaactggca ttctgttgct ggcgctgatt
    67081 ccttttttag tttttcccct tctggcacac aatctgagca gttataacgc tgactattct
    67141 ttgtatgtcg tcgattactg tccggacaac catactgcga cctggggaat caactataca
    67201 aaaatattgg tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta
    67261 gccagcgaac tatggccgtt aatagcatta ttatgtctgg catcgctatg ctgcaactta
    67321 cttctactga ctctggatga gtataattac accatctggt atatcagtcg cgggattgaa
    67381 gtttccagta aactgtttgt tgtgtctttt ctgatttata acatttttca ggaactgcaa
    67441 ctctccagca aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt
    67501 ttcaacagcg tagagtcatt attgtcgcga cctgttgtta aggacttttg tgtcatgctg
    67561 gttgatatta atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa
    67621 gtgctggttt caattgtcga tattatccag caaagcatcc gccccgatga tattttagcg
    67681 cgactggagg gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa
    67741 atcattgcgg aacgtatgcg taaaaatgtc gaactcctga ccggctttag taacagatat
    67801 gatgttcctg aacaaatgac catcagtatt ggcacggttt tttcaacggg tgacacgcgt
    67861 aatatctcgc ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg
    67921 ggcaacaaag tgattattca tcatatttaa gtgcaaaaat attcagagcc atgctttttg
    67981 cgtggctttt gcatacaatt tattactatt acccctaaat ttcccctccc cagcggcgca
    68041 gagatgagta taattagcgc ccctgtgcca ggccgcaatc gaactttatc tggttttctc
    68101 gtttcactaa ccgaaggagt gccatttatc atgaaattgc accatagaat gctccggcat
    68161 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
    68221 agcgacagag caatgagcgc ctatctgcgc tatattgtgc agaaagcaga ctcctccttt
    68281 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
    68341 gagcagtcgg aagtgtcgcc agaaaagcgg cgcgccatct gcgaggcttt tgagtctgcc
    68401 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
    68461 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcgctatt acccgctttt
    68521 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
    68581 gaagaaaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
    68641 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
    68701 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
    68761 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
    68821 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atctgcggaa tatcttttat
    68881 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
    68941 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
    69001 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
    69061 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
    69121 cggatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcaatgact
    69181 ggactgtata atcgcaaaat tttaacccct gaactggaac agcggttgca gaaactggtg
    69241 caatccggtt cttcggtgat gtttattgct attgacatgg acaagttaaa gcaaataaat
    69301 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
    69361 cagtcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
    69421 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
    69481 caacatatcg cgccgcagaa agagatcggc ttctcttccg gtatttatgc gatgaaagaa
    69541 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
    69601 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcat
    69661 gaaatgactg aaatggctaa aggaagcgtg acgcatcaac ggttaatcgc tttattatca
    69721 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
    69781 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaggtcaaa
    69841 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
    69901 agccaactcg ccagccatat aggcggatta cgcgcttcgt tggccagccc ggcggaagtc
    69961 gatgaactca caggctgtgt cttcggcgcg ctcccccctt tcagcttcca tccaaaactc
    70021 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggc
    70081 atgctggata aatccgttat tttgaaaacc gcggattatt tgcgcattgc gcaaccagaa
    70141 ctggtcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
    70201 aaccagcaca accgcaatgg caaaaaacga tgacgagata atcagtgtct cgacgaacat
    70261 ttgatcgttc atagtatgcc cccgggagat agctgttttc acgttattgt tagcgtgcac
    70321 caatggcagt ttgatgacag ttcgcctgat tttttatgca aaaaacgtaa agattttcta
    70381 cttccttcct gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac
    70441 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
    70501 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
    70561 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
    70621 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccctga ttcactcgtt
    70681 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtt attgtctcct ggctgggtgg
    70741 gcgcggcgtg acgttaatgg gcagccagcc gcaactggtt gctgggctgc tggtgggcac
    70801 tgttttgggc gtggcgctgt ttcgcggcgt accggttggc ccgcttattg ccgcgggtct
    70861 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
    70921 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
    70981 gcaactttct gtacagtatc gcctttcact aatccctgaa gcgccataat cagctgtaac
    71041 tgctgtcgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg ggcaatattg
    71101 cgttcactca tcgcaaaaaa gcaagcccat tgccccaatg acccccactc gacgggctct
    71161 ttcgccatca tctccaccat cgcgcggatt ttaggatgag aagaaacggg taggtgcagc
    71221 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
    71281 ggctcagcac gctgagtcgt cgtcctgttt gccagagtta aaatcaattc ccggcataag
    71341 ggcgatattt tcaatgtgca acaggttgtc ggcattatca ctgcgctggg ttcgataaac
    71401 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc tcccggtatc
    71461 caaaccgcat attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca
    71521 ccatgtagcg ccagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
    71581 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
    71641 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
    71701 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
    71761 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
    71821 tgatgattgc cacaacatta cgcgtcacct ttaccggcgc agcgccgtta ctggatacga
    71881 ttcgttccgc ttactcgctg acgacagcgc aaaccggctt attaaccacc ctgccattat
    71941 tggcctttgc gctaatctca cctttggctg ccccggtggc gcgacgtttt ggtatggaac
    72001 gtagcctgtt tgccgcgtta cttttgatct gtgcaggtat cgcaattcgt tctctccctt
    72061 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg
    72121 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
    72181 catattccct gacaatggga gctgcagcgg cactgggatc ggctatggtc gtgccgctgg
    72241 ctttgaacgg ttttggctgg caaggcgcgt tgttcatgct gatgtgtttt cctctgctgg
    72301 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc
    72361 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc
    72421 ttgggatcaa ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca
    72481 gtcgcggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
    72541 cagctcccgg tttgctgatc cctcttttct tacatcatgt gaaagatcag cgtggtattg
    72601 cagcgttcgt tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc
    72661 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
    72721 tgacgttcat tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg
    72781 cacaatccgt cgggtatttg ctggcagcct gtgggccgcc gctgatgggt aaaatacacg
    72841 atgctaacgg tgactggtcc gtaccactga tgggtgttgc catactttca ctactgatgg
    72901 cgattttcgg actttgcgcc gggagagaca aagaaattcg ctaatatccg gtgctatagt
    72961 gacgtgacaa atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg
    73021 tttatgatcc ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc
    73081 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
    73141 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
    73201 atcttgcaga actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
    73261 aacagcccct gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc
    73321 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
    73381 gccggatggt accgtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
    73441 caattgttgc tgaccccttg cgctgtctgc actggaatga gcgtcccgcc cttctgctgg
    73501 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtgc ttctggctga
    73561 gaaggggttg aacaaccagc caggaccagc aagcaaggca gaacaaaact gataattttc
    73621 atttattgat ctcacatatt tatccagact tagagtatcg cggtatcgtt ttgttttgca
    73681 gcactatttt tattacattc actcaaatca tattacgcct tgtttcatct ttgttgatga
    73741 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
    73801 tttcgtgtca gatcagatta tcgcccgcgt ctcgcaatcc cttgccaaag aacagtcact
    73861 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
    73921 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
    73981 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
    74041 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
    74101 tgcggcacgc aatcttggca tcaccacatt tctgagcacg ccaattcact taccggatgg
    74161 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
    74221 ggaacaggtt ttacagttat tcgctggact gattgcacaa tatattcaaa aagaggcgct
    74281 ggttgaacag ctgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
    74341 aaccgggcta ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt tttctctcgc
    74401 ccggcatctt aaccataaga taatgatcgc gtttatcgat ctggataact tcaaattaat
    74461 caatgaccgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
    74521 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
    74581 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgta ttcaacagca
    74641 aatacgtgga gaatatcact taggtgatgt tgatttgtat tatcccggtg ccagtcttgg
    74701 catagtagaa gtcgatcctg aaacaaccga tgcagacagt gccctgcatg ctgccgatat
    74761 tgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc atccagcgct
    74821 acattcctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
    74881 tttccggttg taatctttat tacagcggtc gtatttttag catggttttt tattggcggc
    74941 tatgctgccc cgggagcata aagatgaaaa aaacaacgat tattatgatg ggtgtggcga
    75001 ttattgtcgt tctcggcact gagctgggat ggtggtaacg tcacctctaa aaaatagcaa
    75061 aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct tcctgtagat
    75121 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
    75181 gactttacca aagccaaaca gtgtgctgat ccatccgccg actacggcac cgactatccc
    75241 cagcagaata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
    75301 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
    75361 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
    75421 gatttgtgac gagcatcacg gaggattttt ttgcggatgg cacagaaatt gcgccatcaa
    75481 cgatcagtga taattaccaa ccacaaacat catgttcgtt ttccgtgtct tgacatcctc
    75541 cacgccctga atgacgagga tccctgctac gttcaggctg tcgcctgaat catttcggtg
    75601 ggttcctgct tcaacgagcg gcctgactgc accatc
//
LOCUS       DAHBHC010000014        73374 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHBHC010000014 DAHBHC010000000
VERSION     DAHBHC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.014"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="M2A35_001242"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="M2A35_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCB7197563.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="M2A35_001243"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="M2A35_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCB7197564.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="M2A35_001244"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="M2A35_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCB7197565.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSYSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="M2A35_001245"
     CDS             complement(1087..1581)
                     /locus_tag="M2A35_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCB7197566.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="M2A35_001246"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="M2A35_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCB7197567.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="M2A35_001247"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="M2A35_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCB7197568.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="M2A35_001248"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="M2A35_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCB7197569.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="M2A35_001249"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="M2A35_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCB7197570.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /locus_tag="M2A35_001250"
     CDS             complement(6745..7032)
                     /locus_tag="M2A35_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCB7197571.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /locus_tag="M2A35_001251"
     CDS             complement(7151..8038)
                     /locus_tag="M2A35_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCB7197572.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALVPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="M2A35_001252"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="M2A35_001252"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCB7197573.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="M2A35_001253"
     CDS             complement(9275..9769)
                     /locus_tag="M2A35_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCB7197574.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="M2A35_001254"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="M2A35_001254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCB7197575.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="M2A35_001255"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="M2A35_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCB7197576.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="M2A35_001256"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="M2A35_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCB7197577.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="M2A35_001257"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="M2A35_001257"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCB7197578.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /locus_tag="M2A35_001258"
     CDS             13861..14520
                     /locus_tag="M2A35_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7197579.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="M2A35_001259"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="M2A35_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCB7197580.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="M2A35_001260"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="M2A35_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCB7197581.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAETRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="M2A35_001261"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="M2A35_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCB7197582.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="M2A35_001262"
     CDS             17948..18874
                     /locus_tag="M2A35_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCB7197583.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGEIGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="M2A35_001263"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="M2A35_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCB7197584.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="M2A35_001264"
     CDS             complement(19225..21444)
                     /locus_tag="M2A35_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCB7197585.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="M2A35_001265"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="M2A35_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCB7197586.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="M2A35_001266"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="M2A35_001266"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCB7197587.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="M2A35_001267"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="M2A35_001267"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCB7197588.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="M2A35_001268"
     CDS             complement(26816..27298)
                     /locus_tag="M2A35_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7197589.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="M2A35_001269"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="M2A35_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCB7197590.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="M2A35_001270"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="M2A35_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCB7197591.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="M2A35_001271"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="M2A35_001271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCB7197592.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30278)
                     /locus_tag="M2A35_001272"
     CDS             complement(29946..30278)
                     /locus_tag="M2A35_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCB7197593.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            30597..31178
                     /gene="mdaB"
                     /locus_tag="M2A35_001273"
     CDS             30597..31178
                     /gene="mdaB"
                     /locus_tag="M2A35_001273"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCB7197594.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            31209..31523
                     /locus_tag="M2A35_001274"
     CDS             31209..31523
                     /locus_tag="M2A35_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCB7197595.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31571..33463)
                     /gene="parE"
                     /locus_tag="M2A35_001275"
     CDS             complement(31571..33463)
                     /gene="parE"
                     /locus_tag="M2A35_001275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCB7197596.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="M2A35_001276"
     CDS             complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="M2A35_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCB7197597.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="M2A35_001277"
     CDS             complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="M2A35_001277"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCB7197598.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(34925..35347)
                     /locus_tag="M2A35_001278"
     CDS             complement(34925..35347)
                     /locus_tag="M2A35_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCB7197599.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="M2A35_001279"
     CDS             complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="M2A35_001279"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCB7197600.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSRELAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            36182..37663
                     /gene="tolC"
                     /locus_tag="M2A35_001280"
     CDS             36182..37663
                     /gene="tolC"
                     /locus_tag="M2A35_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCB7197601.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            37811..38482
                     /locus_tag="M2A35_001281"
     CDS             37811..38482
                     /locus_tag="M2A35_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCB7197602.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            38488..39648
                     /locus_tag="M2A35_001282"
     CDS             38488..39648
                     /locus_tag="M2A35_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCB7197603.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQIEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="M2A35_001283"
     CDS             complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="M2A35_001283"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCB7197604.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            40617..41390
                     /gene="zupT"
                     /locus_tag="M2A35_001284"
     CDS             40617..41390
                     /gene="zupT"
                     /locus_tag="M2A35_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCB7197605.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(41448..41618)
                     /locus_tag="M2A35_001285"
     CDS             complement(41448..41618)
                     /locus_tag="M2A35_001285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197606.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="M2A35_001286"
     CDS             complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="M2A35_001286"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCB7197607.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(42636..42784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42906..43196
                     /gene="ubiK"
                     /locus_tag="M2A35_001287"
     CDS             42906..43196
                     /gene="ubiK"
                     /locus_tag="M2A35_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCB7197608.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            43479..44030
                     /locus_tag="M2A35_001288"
     CDS             43479..44030
                     /locus_tag="M2A35_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCB7197609.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            44090..46594
                     /locus_tag="M2A35_001289"
     CDS             44090..46594
                     /locus_tag="M2A35_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7197610.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            46610..47359
                     /locus_tag="M2A35_001290"
     CDS             46610..47359
                     /locus_tag="M2A35_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7197611.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            47361..48425
                     /locus_tag="M2A35_001291"
     CDS             47361..48425
                     /locus_tag="M2A35_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7197612.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(48468..48668)
                     /gene="glgS"
                     /locus_tag="M2A35_001292"
     CDS             complement(48468..48668)
                     /gene="glgS"
                     /locus_tag="M2A35_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCB7197613.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            48937..49566
                     /locus_tag="M2A35_001293"
     CDS             48937..49566
                     /locus_tag="M2A35_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCB7197614.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            49593..50831
                     /locus_tag="M2A35_001294"
     CDS             49593..50831
                     /locus_tag="M2A35_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCB7197615.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTQKKKPQRCRRRLSLS"
     gene            50804..51241
                     /locus_tag="M2A35_001295"
     CDS             50804..51241
                     /locus_tag="M2A35_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197616.1"
                     /translation="MQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFK
                     LALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSA
                     ALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            51480..51539
                     /locus_tag="M2A35_001296"
     CDS             51480..51539
                     /locus_tag="M2A35_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCB7197617.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            51856..51915
                     /gene="ibsE"
                     /locus_tag="M2A35_001297"
     CDS             51856..51915
                     /gene="ibsE"
                     /locus_tag="M2A35_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCB7197618.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(52035..53468)
                     /gene="hldE"
                     /locus_tag="M2A35_001298"
     CDS             complement(52035..53468)
                     /gene="hldE"
                     /locus_tag="M2A35_001298"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCB7197619.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(53516..56356)
                     /gene="glnE"
                     /locus_tag="M2A35_001299"
     CDS             complement(53516..56356)
                     /gene="glnE"
                     /locus_tag="M2A35_001299"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCB7197620.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(56379..57680)
                     /gene="ygiF"
                     /locus_tag="M2A35_001300"
     CDS             complement(56379..57680)
                     /gene="ygiF"
                     /locus_tag="M2A35_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCB7197621.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            57922..58542
                     /locus_tag="M2A35_001301"
     CDS             57922..58542
                     /locus_tag="M2A35_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCB7197622.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            58606..59844
                     /gene="cca"
                     /locus_tag="M2A35_001302"
     CDS             58606..59844
                     /gene="cca"
                     /locus_tag="M2A35_001302"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCB7197623.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(60007..60828)
                     /gene="bacA"
                     /locus_tag="M2A35_001303"
     CDS             complement(60007..60828)
                     /gene="bacA"
                     /locus_tag="M2A35_001303"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCB7197624.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(60919..61287)
                     /gene="folB"
                     /locus_tag="M2A35_001304"
     CDS             complement(60919..61287)
                     /gene="folB"
                     /locus_tag="M2A35_001304"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCB7197625.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            61392..62009
                     /gene="plsY"
                     /locus_tag="M2A35_001305"
     CDS             61392..62009
                     /gene="plsY"
                     /locus_tag="M2A35_001305"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCB7197626.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(62022..62954)
                     /gene="ttdR"
                     /locus_tag="M2A35_001306"
                     /gene_synonym="dan"
     CDS             complement(62022..62954)
                     /gene="ttdR"
                     /locus_tag="M2A35_001306"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCB7197627.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            63161..64072
                     /gene="ttdA"
                     /locus_tag="M2A35_001307"
     CDS             63161..64072
                     /gene="ttdA"
                     /locus_tag="M2A35_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCB7197628.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            64069..64674
                     /gene="ttdB"
                     /locus_tag="M2A35_001308"
     CDS             64069..64674
                     /gene="ttdB"
                     /locus_tag="M2A35_001308"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCB7197629.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            64723..66186
                     /gene="ttdT"
                     /locus_tag="M2A35_001309"
     CDS             64723..66186
                     /gene="ttdT"
                     /locus_tag="M2A35_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCB7197630.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(66229..67242)
                     /gene="tsaD"
                     /locus_tag="M2A35_001310"
     CDS             complement(66229..67242)
                     /gene="tsaD"
                     /locus_tag="M2A35_001310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCB7197631.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            67480..67695
                     /gene="rpsU"
                     /locus_tag="M2A35_001311"
     CDS             67480..67695
                     /gene="rpsU"
                     /locus_tag="M2A35_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCB7197632.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            67806..69551
                     /gene="dnaG"
                     /locus_tag="M2A35_001312"
     CDS             67806..69551
                     /gene="dnaG"
                     /locus_tag="M2A35_001312"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCB7197633.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     MLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            69746..71587
                     /gene="rpoD"
                     /locus_tag="M2A35_001313"
     CDS             69746..71587
                     /gene="rpoD"
                     /locus_tag="M2A35_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCB7197634.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(71666..72172)
                     /gene="mug"
                     /locus_tag="M2A35_001314"
     CDS             complement(71666..72172)
                     /gene="mug"
                     /locus_tag="M2A35_001314"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCB7197635.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQHGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            72297..72372
                     /locus_tag="M2A35_001315"
     tRNA            72297..72372
                     /locus_tag="M2A35_001315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72330..72332,aa:Ile,seq:cat)
BASE COUNT        18479 a        19355 c        18129 g        17411 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtac gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggta
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggta ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgagat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gagatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgtgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgaaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca cctgagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tataagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactaca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc acccgctgat gcaacaacgc cagctacatg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaactccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg cattgtcgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaat cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccaa
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagttcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct ccaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
    30241 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcataaaaat
    30301 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
    30361 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
    30421 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
    30481 cagagcaaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
    30541 ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt taaaaaatga
    30601 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
    30661 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
    30721 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
    30781 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
    30841 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
    30901 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
    30961 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
    31021 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
    31081 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
    31141 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
    31201 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
    31261 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
    31321 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
    31381 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
    31441 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
    31501 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
    31561 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
    31621 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
    31681 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
    31741 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
    31801 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
    31861 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
    31921 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
    31981 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
    32041 cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
    32101 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
    32161 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
    32221 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
    32281 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
    32341 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
    32401 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
    32461 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
    32521 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
    32581 agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct
    32641 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
    32701 acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt
    32761 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat
    32821 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc
    32881 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
    32941 caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag
    33001 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
    33061 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
    33121 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
    33181 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
    33241 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
    33301 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
    33361 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
    33421 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
    33481 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
    33541 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
    33601 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
    33661 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
    33721 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
    33781 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
    33841 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
    33901 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
    33961 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
    34021 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
    34081 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
    34141 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatagta tccagcgtaa
    34201 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
    34261 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
    34321 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc
    34381 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
    34441 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
    34501 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacgcgct
    34561 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
    34621 tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat
    34681 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
    34741 gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg tttaccccta
    34801 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
    34861 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
    34921 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
    34981 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
    35041 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
    35101 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
    35161 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
    35221 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
    35281 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
    35341 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
    35401 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gccagttccc
    35461 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
    35521 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
    35581 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
    35641 cttcgcgacg ggcaacatct tccacacttt caccctcttc tatcatcccg gcaaccatct
    35701 ccagtagcca gggggtttca ctggtgtcgt atgcggcaat ccgaatctgc tcaatcagca
    35761 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
    35821 tttccctccg tacctcatga ctcatttgcc cgttgaataa acgatgacga aatctataaa
    35881 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
    35941 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
    36001 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
    36061 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta
    36121 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
    36181 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca gttcgttgag
    36241 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
    36301 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
    36361 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
    36421 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
    36481 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagctgca gggattcagg acgtcacata
    36541 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
    36601 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
    36661 tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc agaacgcccg
    36721 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc ttgataacgc
    36781 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
    36841 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
    36901 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
    36961 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
    37021 cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg acgacagcaa
    37081 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
    37141 taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc aactggaaag
    37201 cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcatctat
    37261 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
    37321 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg caaccaccac
    37381 gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga ttaatcagct
    37441 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
    37501 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
    37561 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
    37621 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
    37681 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
    37741 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
    37801 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
    37861 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
    37921 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
    37981 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
    38041 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
    38101 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
    38161 cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg
    38221 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
    38281 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
    38341 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
    38401 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
    38461 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
    38521 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
    38581 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
    38641 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
    38701 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
    38761 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
    38821 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
    38881 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
    38941 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
    39001 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
    39061 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
    39121 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
    39181 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
    39241 ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg
    39301 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
    39361 gatcatccgc aaatagaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
    39421 gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
    39481 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
    39541 ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg
    39601 atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc aatgatgccg
    39661 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
    39721 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
    39781 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
    39841 cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga ttcgctttca
    39901 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
    39961 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
    40021 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
    40081 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
    40141 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggcgctaac agctcaacca
    40201 gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
    40261 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    40321 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    40381 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    40441 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    40501 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    40561 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
    40621 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    40681 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    40741 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    40801 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    40861 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    40921 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    40981 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    41041 cactggccgt cgctttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg
    41101 cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa
    41161 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
    41221 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    41281 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    41341 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
    41401 agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
    41461 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    41521 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
    41581 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
    41641 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
    41701 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
    41761 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcggg gcaaaataaa
    41821 aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc
    41881 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
    41941 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
    42001 cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa
    42061 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
    42121 aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa
    42181 cggtcgtaat acgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
    42241 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
    42301 gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg
    42361 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
    42421 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
    42481 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
    42541 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
    42601 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
    42661 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
    42721 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
    42781 aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc acataattct
    42841 ggtttatgac ttacccttat cgcactacaa tggtactcaa cacctatcag tacagggaaa
    42901 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
    42961 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
    43021 ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc
    43081 tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatgagcgaa ctggaaaatc
    43141 gcagtactga gattaaaaag cagccagatc cagagactct ccccccaacg ctgtaaacat
    43201 ttgtctttaa tgcactgtaa attatgaatt aatcctctaa tttacagtgc tttctattac
    43261 cttatcgctt aattccattc agaatatcat cacaaaaatg aatgagtgca attgaaaata
    43321 taattgttat gtatttttta agttttactt aatccatatt tagataaact aatttcataa
    43381 aacccgaaaa gtaaaatata atatttgaat gattagcaat ccttaaataa cagttactaa
    43441 tcattcattt tctacttata tttatataaa ggaatattat gtctgctttt aaaaagtcac
    43501 ttcttgttgc tggcgtagca atgattcttt ccaataacgt atttgctgac gaaggtcacg
    43561 gcattgttaa atttaaaggt gaagttatct ctgctccatg ttcaattaaa ccaggtgacg
    43621 aagacttaac tgtaaatctg ggtgaagtag cagataccgt tctgaaaagc gaccagaaat
    43681 ctttggctga gcctttcact attcacctgc aagactgcat gttgagccag ggtggaacca
    43741 cttattcaaa agcgaaggtt actttcacca cagcgaatac tatgacgggt cagactgacc
    43801 tgctgaaaaa caccaaagaa actgaaatcg gtggtgccac aggcgttggc gtacgtattc
    43861 tggatagcca gagcggcgaa gtgactttag gtactccggt agtaattaca ttcaacaata
    43921 ctaacagcta tcaggaactg aacttcaaag ctcgtatgga atccccaagc aaagatgcta
    43981 ccccgggcaa cgtttacgct caggctgact ataagattgc ttacgagtaa tcacattagg
    44041 ccgggttcgc ccggccacat cattaacgga ttaatgataa gtggaacaga tgtataaaaa
    44101 attaaaatta accacaataa gcgaattgat taaaaatatt tattgttcat tatccgttct
    44161 catcattggt tgtgcgtcag cttatgccgt tgaattcaac aaagatttaa tcgaagccga
    44221 agatcgtgaa aacgttaacc tttcccaatt tgaaactgat ggccaattac ccgtaggtaa
    44281 atactcatta aacgctctta ttaataataa aaggacgcca atccatctgg acctgcaatg
    44341 ggtattaatt gataaccaaa ctgcagtttg tctgactcca gagcaattaa cattattagg
    44401 ctttactgat gagattattg aagtggctca gcaaaacctg atcgatggtt gttaccctat
    44461 cgaaaaagaa aaacaaatta caacttatct cgataaaggg aaaatgcaat tatccatatc
    44521 tgcacctcag gcatggttaa aatacaaaga tgcaaactgg acgcctcctg aactttggga
    44581 tcatggtatt gctggggcat ttcttgacta caatttatat gcctctcatt atgcaccaca
    44641 tcagggcgat aattcacaaa atataagttc ctatgggcag gctggggtta atcttggggc
    44701 ctggcgcctg cgtactgatt accagtacga tcagtcattt aacaatggca aaagccaggc
    44761 gaacaacctg gattttccgc gtatttattt gtttcgccca atcccagcaa ttaatgcaaa
    44821 actaactata ggtcaatacg atactgaatc ctctattttc gactctttcc atttttctgg
    44881 cgtttcgttg aaaagcgatg agaatatgtt gccgcctgat ctacgtggtt acgcaccgca
    44941 aatcacgggt gttgcacaaa cgaatgcaaa ggtcactgtc tcacagaaca accgtattat
    45001 ttatcaagaa aatgttcctc ctggcccatt tgctattact aatttattca atacattaca
    45061 ggggcaactg gacgtcaagg ttgaagaaga ggacgggcaa gtcacgcaat ggcaagttgc
    45121 atctaatagt attccttatc tgacgcgtaa agggcagatt cgctacacca ctgctatggg
    45181 taaaccgacc agcgttggtg gtgattcctt acaacaaccc ttcttctgga ctggtgaatt
    45241 ctcatggggt tggctgaaca atgtatccct gtatggtggt tcagttttaa caaaccgtga
    45301 ttatcaatct ctggctgccg gcgttggctt taaccttaac tcattgggtt cattatcttt
    45361 tgatgtcaca cgatctgatg ctcagttgca taatcaggat aaagaaacgg gttatagcta
    45421 ccgcgctaac tattcaaaac gttttgaatc taccggtagc cagctcactt tcgctggtta
    45481 ccgtttctct gataaaaact ttgtgacaat gaatgaatat atcaatgaca ctaaccatta
    45541 cacgaattat cagaatgaaa aagagagtta tattgtcacg tttaaccagt atcttgaatc
    45601 attaaggtta aatacatacg taagtttggc tcgtaatact tactgggacg ccagcagtaa
    45661 tgtgaattat tcattatcac ttagccgcga ttttgatatc gggccattaa aaaacgtctc
    45721 cacttctcta acatttagcc gaataaactg ggaagaagac aaccaggatc aactgtacct
    45781 aaatatttcg attccctggg gaaccagtag aacattgagc tatggtatgc aacgaaatca
    45841 ggataacaag atttcgcata ctgcttcgtg gtatgactct tccgatcgaa ataattcctg
    45901 gagcgtttct gcttcaggcg acaatgatga attcaaagat atgaaagcgt cactacgcgc
    45961 cagttatcag cataataccg agaacggtcg tctctatctc tccgggacat cacagcgaga
    46021 cagttattat tctctgaatg ccagttggaa tggttcattc acggcgactc gtcacggagc
    46081 cgctttccat gactatagtg gtagtgctga ctcgcgtttt atgatcgacg cagatggtgc
    46141 agaagatatt ccgttgaaca ataaacgcgc ggtaactaat cggtatggca tcggagttat
    46201 tccatcagtc agcagttaca taacaacatc attaagcgtt gatacccgaa atcttccaga
    46261 aaatgtggat atcgaaaact cggttatcac caccacctta accgaaggtg ctattggcta
    46321 cgccaaactt gatacccgca agggctatca aatcatgggg gttattcgcc tggcagatgg
    46381 tagtcaccca ccactgggga ttagcgtaaa agataaaacc agccataaag aattaggact
    46441 ggttgctgat ggcggctttg tatacctcaa cggcattcag gatgacagca aacttacttt
    46501 acgctggggt gataaatcct gttttattca accacccaat agcagcaact taaccaccgg
    46561 aacggttatt ttaccgtgta ttagccaaaa ttaattcagg atgaaactaa tgcgctactt
    46621 aaacacaaag aatctcattg cagcaggagt attactcgcc tgcatgagca gcattgcctg
    46681 gggtgccatc atcccggacc gtacccgtat cataatgaat gagtcagata aaggggaagc
    46741 attaaaactt actaatcaaa gcaaaaatct accctatcta gcgcaaacat ggatagaaga
    46801 tacgaaaggc aataaatcac gtgactttat tgtcacggtt ccccctatgg tacgtttaaa
    46861 tcccagtgag caaattcaaa taagaatgat aagtcaggaa aaaattgctc aactgcctaa
    46921 tgacagagaa acgctcttct attttaacgt gcgagaaata ccgccaaaaa cagataaaaa
    46981 aaatgtgatg caagtcacta tgcagcacgc attgaaacta ttctggcgac caaaagctat
    47041 tgaattagaa gatgatgggg taatgtctta cgaaaaagtt gaaatcatcc gtagaaatga
    47101 tggttcgatc cgctttaaca ataagatgcc ttatcacgtt acgctgggtt atatcggaac
    47161 aaacggaatc acaatgttgc cacaaacgca aagtttaatg gtaacccctt atagccatgc
    47221 agatacgcag tttaaaaatg ttccgtcagc ttttcaggtt ggttatatca atgattttgg
    47281 tggattaagt ttttatgaaa taaactgccc gacggttaat aactcttgta atgtttccgt
    47341 agccaaacga gataaataat atgcgctact tattaattgt aattatattt attataggtt
    47401 ttagtgcatt acctgtatgg gcaatggact gctatgctga acatgaaggg ggaaataccg
    47461 tagtcattgg ttacgtacca agaatttcta tccccagtaa tggtaaaaaa ggtgataaga
    47521 tctggcaaag cagtgagtat tttatgaatg tcttctgtaa taatgcatta cctgccccat
    47581 cacctggaga agaataccca tcagcctgga ctaatataat gatgttttta ccaggcggtc
    47641 aggactttta taatcagaac tcctatattt ttggcgtaac atacaatgga gttgattatg
    47701 attcgactgc ccctaatgct atcgcagcac cagaatgcat tgatataaaa ggagcgggta
    47761 cctttaataa tcactataaa aaccccgctg tctgtagtgg aggtcctgag cctcaattat
    47821 cagtaacctt tccagctcgc gtacaactct atattaaatt agcaaaaaat gccaatagag
    47881 ttaataaaaa cctggtgtta cctgatgaat atatcgctct agagtttaaa ggtatgagtg
    47941 gcgcaggagc tatagaagta gacaaaaatt tgaccttcag gattcgtgga ttaaataata
    48001 ttcatgtcct tgactgcttt gttaatgtcg cactggaacc agctgatggc gttgttgact
    48061 ttggtaaaat aaattcccga acaattaaaa ataccagcgt tagtgagacg tttagtgtag
    48121 tcatgacaaa agatcctggt gcggcctgta ctgagcaatt caatatttta gggagctttt
    48181 tcactacgga tattttgagt gattatagcc atctggatat gggtaatgga ctgctattga
    48241 agatatttca taaagatgga acagcaacgg aatttaaccg cttctcacaa tttgcttctt
    48301 tttcatcgtc tagtgcacct tcggtcaccg caccattcag ggcagaactt agtgcgaatc
    48361 cggcagagac tgttgttgag ggaccgttta gtaaagacgt catcctcaaa atcacctata
    48421 actagcatct aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac
    48481 tccagctctc ttgcctgcat catctgttga caataccagg cataacgtgc gcaaaaccag
    48541 gttctatgtt cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct
    48601 gcgtgcattc tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta
    48661 tgatccataa gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga
    48721 tttcagtatt ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc
    48781 ccgatcacac tttttagtga aaaataaaaa accatattaa aaaactttct cttccagaac
    48841 ttctctaaca tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt
    48901 aaatattgcg tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat
    48961 accccttatc ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg
    49021 cttatctgcg gtcacatgct ctccggtgct cttgatgcac atcttgacca ttatgattcc
    49081 ataaccacag gtcatattag ccaggcactt cattatctca atatcggaag attgccagct
    49141 ctcgtcgttc tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat
    49201 gcctgcatca tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta
    49261 ctgtttacga ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc
    49321 gaccatagtt cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt
    49381 catcaagcca cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata
    49441 cattatttac tactggagcc tgaagaagga aaatttttca ctaaaggaga caaagtgtta
    49501 attatttgcc gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata
    49561 ttgtaattta ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct
    49621 cctggatgtt taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg
    49681 ccaggctcta tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc
    49741 aaaaagtggt gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca
    49801 tcaatatgaa tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga
    49861 aagatcgtat gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag
    49921 aaggcatcgc cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac
    49981 gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca
    50041 tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag
    50101 aagacctgtc gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga
    50161 cctcgaaaga acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga
    50221 ctcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg
    50281 cggtgcgtga gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag
    50341 cgtttatgac gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac
    50401 gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg
    50461 aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg
    50521 aggctgaacg tgaagttcgt attaaagaga tcgaacaaca gcaggtcacc gaaatcgcca
    50581 accagacgaa atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc
    50641 gtgctaacct tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc
    50701 agaccgcaga agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag
    50761 aaaccaaagc ggttgaactg acccagaaaa agaagccgca gagatgcagg cggcggctat
    50821 cgttgagtta gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc
    50881 gctgaacgat gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc
    50941 cttgttgcag gcgctacctg cggtaataga aaaatccgtt gagccgatga aatctattga
    51001 cggtatcaag attattcagg tcgatggtct gaatcgtggc agcgctgcgg gtgatgcaaa
    51061 cacgggtaat gtggggggcg gaaacctggc ggaacaagca ttatcagccg ctctctctta
    51121 ccgcacacag gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc
    51181 actggcggca ttgacttcac ccttaacctc aacaactccc gtcgccgaaa acgtagaata
    51241 aattttcagc tatgaacgtc cctggcacaa aaagccgggg attttttata tctgcgttcc
    51301 gctaaaaact gcaaatgatc aggtcgttgc aacgttttgc gtgaccgctc ggggaaggca
    51361 aaactgcctc tgggaaagcg ttgcgcgagg tccggcgctc atcaacaatc ggggagtcag
    51421 caaagggctg aaacgggaaa gcccctcccg aggaaggggc cttacagaag gaaagggtta
    51481 tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac
    51541 agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa
    51601 aaaatcagcg tcaagaaggt ttcattaaca catatccctg gcccaaaaag ccggggattt
    51661 tttatatctg cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg ttttgcgtga
    51721 ccgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg gcgctcatca
    51781 acaatcgggg agtcagcaag gggctggaac gggaaagccc ctcccgagga aggggcctta
    51841 tagaaggaaa gggttatgat gaagttcgtc atcatactgg ttgtgttgtt actgttaagt
    51901 ttcccgactt actaacaact gatcagaggg gggagaaatc ctcccttacc cttgttcctt
    51961 tactttaggt tgaaaaaaca acagcgtcaa taggcctgcc tgctacgaag cgagatctgt
    52021 gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgatgat gttagtggtc
    52081 gagcaaccgt cttcgaagtt gagcaccagc acttcgccgc cgttggccca gacttcttta
    52141 ctcccggcaa tttcttctgg tttatagtcg ccgcctttca ccagcagatc tggcaggatc
    52201 ccggcgatca gacgctgcgg cgtgtcttct tcaaacgaca ctacccagtc gaccgcttcc
    52261 agtgcgccca gcacaatcat acgctgctcg agtgggttta ccgggcggga atcccctttc
    52321 agccgtttgg tggaggcatc gctgttcaca gcgacaatca gacggtcacc cagtttgcga
    52381 gcatttgcca gataggagac gtgcccggcg tgcaggatgt caaagacacc gttggtcatt
    52441 accacctttt cgccgcgttt acgcgctgcc gctacagcaa gcttcagttc ctcttcggtc
    52501 atcacaccaa agccggtatc tgcgcgccca cgcactgcat tttccagctc gatcggcgaa
    52561 accgtggagg ttcccagttt gccgaccacc acgccagccg ccgcattggc aaagaagcag
    52621 gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat caccgtgtcg
    52681 cccgcaccgg taacgtcata cacttcctgc gcctgggttg gcatatgcag cggcgcttta
    52741 cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc cgagagttcg
    52801 taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt acatttaccg
    52861 acaacagctt caaattccga gagattcggc gttaacagcg tagcgccgcg gtagcgctca
    52921 aaatcggtac cttttggatc aatcagcacc ggaacgcccg ctttacgcgc cagttggatc
    52981 atctgctgta cgcttgccag cgcacctttg gcgtagtcag aaagcaccag cgcgccaatc
    53041 gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc ttcgaaacct
    53101 tcttcaaaat ccaggcggat cagctgttgg ttgcgggaaa gtacccgtaa tttggtgata
    53161 gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc cagagattta
    53221 ctcagcgcac gcgccgcatc gtcaatgccc gtcagcccga ccaggcgagc attggcaccg
    53281 agagaagcga tgttcattgc cacgttagcc gcgccgcccg gacgttcttc gatggtattg
    53341 actttaacca cgggcaccgg cgcttccggc gagatacggc tggtgggacc gtaccagtaa
    53401 cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc tggcagcgtt
    53461 actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc atacttcatt
    53521 cttccaccag ccacttctgc cagcttgccc gcaccagttc acgctctgcg gtgaagcaat
    53581 cctccggcac atggcccggc aattcctgta atgccagatg atgaagttca tcgcgcagcg
    53641 tagtataagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg ttttgcgcca
    53701 gtagttccag aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt
    53761 agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccgcct tcatcagctt
    53821 tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc gcgcattttc tcgcgcattt
    53881 cccgcacttc cgtttgcaga gttttacctt cacgcggcag cgtcataatc tcgcgacgca
    53941 ccgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac tacccgcgca cgcaccagcg
    54001 cctgatgttc ccacgtccag gcctcgtttt tctgataatc ggcaaatgct tctgcggatg
    54061 tcaccagcat tcccgcggcc ccggacgggc gtagccgtgc atccacttca tacaaaatgc
    54121 cggaagaggt acgcgtactg aacagatgca taatgcgttg cgccagacgc agataaaact
    54181 gtcgcccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa tcatggagga
    54241 agatcaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg ccgtagccga
    54301 ccaccgcaaa accacggcct tcgcgatcgt tgaggtgatt tggcttaccg tagcgggcca
    54361 ccatttgaac ccacgcctgc tgaacgacgg catctatcat agcctctgcc agccaggtta
    54421 agtgatcgct cactttcatc accggtagcg taccagcgat atccgctgcg gcgatgcgta
    54481 acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg tcatcttccg
    54541 gcacgcgcag caaatactgg cgcaactcat cgcggtaggc atcggtcgcc gtcggctggt
    54601 aaagggtgtt tggatcgagc aattcatcca gcaataatgg ataacgcgcc agttggctgg
    54661 caatcatcgg cgacgcggca cacagagaaa tcaaatgttt aagcgccgcg gggaattcac
    54721 tgagcaattc taaataggtg gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg
    54781 acagcgtaac ggcagcgtct tcacgcgcac agacatcact tagcagatgc ggcatcagat
    54841 ggtcgagcac ctgacgtcct cgcggcccga tggtgcgctt atccagctct ttgcggaaat
    54901 cggcaatcag cgttagcacc tgtttgcgat catcctcgct aagatgcgcc agcactggcg
    54961 tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc gacagcgact
    55021 cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg cgcacattgg
    55081 tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc atcgcccacg
    55141 ccagccgcgc acggttaagc tcatcagaag gaagcgtctg ggtttgttcg tcgttaatgc
    55201 tttgcagcag gttttccaga cgccgcagga acagatacgc cactcgcaat tgttcagcat
    55261 cgttttcaga aagcagatgc agcgcggcaa tgacgctgag cgttggcagt aaagagcgcg
    55321 actgcagcga cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg ataaattcaa
    55381 tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt cgacgcactt
    55441 cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac
    55501 gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg ccttcgctat
    55561 cgcccataat ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc tgctcctggt
    55621 aataatcctc caacgcggca aagctcagca ccagcgggcc actttcgcca aacggacgca
    55681 gccgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccaac actttaatca
    55741 gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccggcgt ccgccctgcg
    55801 tacaaccatg ttccggccag gcaaaaatca gatcgatatc tgaggagaaa ttcagttccc
    55861 caccgcccag cttacccatg cctaaaatca gcagcggttg cgcttcgccc tgcgcattgc
    55921 acggcgttcc ccactcgcgg cagcaagcgt catacagcca gtcacgcgcg gcgacaatca
    55981 gcgtctccgc cagatggctg agctgctgca atatgctctc ttcagtaacc aaagccagcg
    56041 tttgcgccca ggcgatgcgc accataatgc gccgccggaa tagccgcagc tcgcgcatta
    56101 acccggcttc gtcactcaca ttgctgagcg cctcctgcaa ccatgccgcg taatgctgcc
    56161 attcgtcggc ctgcggcgat tggctttcca gttccgtcag ccactctgga tgcgcaatca
    56221 cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgcg ctaagtgatt
    56281 tctcggctaa aggctctggc agccgctcaa caacggtctg ccagtactgc tgtaacggtg
    56341 aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc gctgtgcaac
    56401 cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcaat gcgttgcccg
    56461 gttgcaatag cgtgacgcag cccctgccag ttctccagcc aggcttgcgc gacgacagga
    56521 tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcgtcagacg tggcaactgg
    56581 tcacggtagc gatcgcccaa cggctggcag aaaacgcttt tcagttcagc ggcatggcgg
    56641 gaaagatgga tatcggcaaa gcgtttgaag gaatcgctca ttttactctg cgctttggca
    56701 tctaaaaacg gctgccaggc tttgcttacc agccattcgg tcaacgccag cttcgccatt
    56761 gccgttttgg tagagtagac cgccgtcacg gcagaaaccg cagaagcgat ggtcgcttcg
    56821 cattgagtca gcaaatcacg taagtgagtg ctcgctttgc gcggcacaat accaccgaac
    56881 agcatcagtg tatgacggac cagaccaatg gctgccagca cctgttcttt cgccgcatcg
    56941 ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacgccag ctcgagcgcc
    57001 gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt ggtcggtttg
    57061 atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt gctcaggctg
    57121 ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg
    57181 tcgccgctaa gcagttccag ttccagctca cagataggtt cagcaaattc ccccgctttc
    57241 acctccccct gatcgagagc gatttcaatt cggctgtcat cgaccgccac cagccatttt
    57301 tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg aagcgagatc ggcgggcaat
    57361 tcgccgttcg gccagacttc cgtcggtaac tgcgccaggt cgagcgtcgg ttcgctcaac
    57421 gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg
    57481 gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg cccacgcagc
    57541 cagttatccg gcgtttcgta gtaaatattc agcaactgca cggggtcatg gtgctcgccg
    57601 cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac aataaacttt
    57661 aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag tgacgatgaa
    57721 cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa attgccatgc
    57781 aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac acagaggatt
    57841 ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg ttccactttc
    57901 catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac tttactcgca
    57961 cttagcgcca ctgccgtctc acacgctgaa gaaacgcgct atgtttctga tgaactgaat
    58021 acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt taacgccggc
    58081 gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt gaaagacagc
    58141 tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag cctgcgctcc
    58201 cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac caatatcgat
    58261 aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg
    58321 atcaacggat taaaagaaga aaatcagaaa ttgaaaaacg agctgattgt cgcgcagaaa
    58381 aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat catgcaatgg
    58441 tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt actgccacac
    58501 ctgatcccaa cgcgcaaacg caaagatcgc tggatgaact aaatcgcctt cttcgttgca
    58561 ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa gatttatctg
    58621 gtcggtggtg ctgttcggga tgcattgtta gggctaccgg tcaaagacag agattgggtg
    58681 gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt aggccgcgat
    58741 tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg taccgaacgg
    58801 aaatccggtt ccggttacac cggttttact tgctatgccg caccggatgt cacgctggaa
    58861 gatgatctta agcgtcgcga tctgaccatt aatgcgctgg ctcaggacga taacggcgag
    58921 attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc
    58981 cccgcttttg gcgaagatcc gttacgcgtt ctgcgcgtgg cgcgttttgc tgcgcgttat
    59041 gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga gatgacccat
    59101 gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacgga aagcgccctc
    59161 accacccgca atccccaggt gttcttccag gtactgcgcg attgcggtgc acttcgcgtt
    59221 ttattcccgg aaattgacgc cctgtttggc gttccggccc ctgccaggtg gcatccggaa
    59281 atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct gagtccgcag
    59341 gttgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac gccgccagaa
    59401 ctttggccgc gtcatcatgg gcatggcccg gcgggtgtta agttagtgga acaactatgc
    59461 cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc tgagtttcac
    59521 gatctcatcc acaccttccc aatgctgaac ccaaaaacca tcgtcaaatt atttgattcc
    59581 atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac
    59641 gtacgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa
    59701 gcctgggaag tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg atttaaaggt
    59761 gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag ttggaaggaa
    59821 caacgttgcc caaagcctga ctgagtataa aactaaacgt taacacgaac catcccctcg
    59881 ccccttcggg gagagggtta gggtgagggg aacagaccag caccggtgcg aacaatcacc
    59941 cccaccccgg ccctctccct acagagcgag ggagaaaacc aggcctgctc ccgaaccttt
    60001 tctcaatcaa aagaacacga cgtacaccgc agccgccaca ataaagcgat aaatggcgaa
    60061 cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac
    60121 cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg tcaggaagcc
    60181 ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg ccagcaggaa
    60241 cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg aaatggtggc
    60301 cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaacgc
    60361 ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc
    60421 ggcggcaatc agcaacaaac cgccaacgac cagtgcatac atcacattta tcgggttaaa
    60481 caatgactta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa tcatccccag
    60541 caaaatgtgg atcagcgtta aacgaccttt gctttctcct tcgtgctgca acgggcggcc
    60601 aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta ccgccagaat
    60661 tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcga accccaacaa
    60721 gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc
    60781 gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct
    60841 aaattattaa aatgtaccgc ttgtccggac tactgcagta agaccaggtt ataaccgttt
    60901 ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct caatgattac
    60961 gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac gcacccacgg
    61021 cgagttgaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt ccaccagcgc
    61081 aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt aactgaggca
    61141 atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt cgatatcgaa
    61201 cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg tgattaccga
    61261 aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct aaccggatac
    61321 cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga attttcaaaa
    61381 cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct
    61441 ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg cgaaccagcg
    61501 gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga gcagccgtag
    61561 cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag
    61621 gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc
    61681 ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt gccatcgcac
    61741 ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga
    61801 gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt tatgtatggt
    61861 ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgtc
    61921 atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca
    61981 aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac ctgatagcct
    62041 tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt
    62101 aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc gctttgtgca
    62161 tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac atcccactcg
    62221 gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc gccgctattg
    62281 gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc caactcccat
    62341 attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact taattcctgt
    62401 aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta
    62461 tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat acgaatatcc
    62521 agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa atgcacctgt
    62581 aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct gcgcccaaaa
    62641 ccaaaactac agccaatacg aatcatcccc tccgggcgcg ttttgatttg cgtgacgtca
    62701 tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca
    62761 ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc cagcgtattt
    62821 tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt cgccgctgcg
    62881 gcgctaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt ggctaaaggc
    62941 cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat ttcgcaaaac
    63001 attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat tcacgaactg
    63061 ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg
    63121 cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg aaagtaataa
    63181 gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca tgatttctac
    63241 ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacgtcgtc
    63301 gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga ttgacctgaa
    63361 tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg gttcccgctt
    63421 cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg caaccgtcaa
    63481 agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac
    63541 cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg atgcagaaat
    63601 cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag tgttaatgcc
    63661 gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt
    63721 aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc
    63781 cgtactctcg cgtaaagcca ttttgcgtcc gattggcagc cgccatccca atccaaaagc
    63841 ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg gtccacaagg
    63901 gctgaccggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc gccatccgtc
    63961 aaccatcggc gttgctgtct ctaccggctg ctgggcgcat cgtcgcggca cactgctggt
    64021 tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc
    64081 ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga tgtgatctac
    64141 ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gccgcttgat cgaactgaaa
    64201 cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc
    64261 aaaaacggcg ataaatggga gatggtttcc gttggcccga caaccagtat gcgtatggaa
    64321 agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg caaaggcgga
    64381 atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctacatgt gattttcccg
    64441 gcaggctgcg cggtgctggc ggcaacccag gtggaagaga ttgaagaagt gcactggaca
    64501 gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg cccgctgatt
    64561 gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa
    64621 cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc
    64681 cggagaggct cgccccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa
    64741 tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat tccggttccc
    64801 gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt gatcgttgga
    64861 ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc catcatcgcc
    64921 atactctctc cctggctgct gttcagcccc gaacagctcg cccagccagg ctttaaattc
    64981 actgcaaaat ccctctcgtg ggcggtttcc ggtttttcta attcggttat ctggctgatt
    65041 ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggctgggacg ccgcattgcg
    65101 ctgattctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc ggtgatgttc
    65161 tccgagctga tcctcgcacc tgtaacaccg tccaactcgg cgcgtggtgc ggggattatc
    65221 tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg
    65281 cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc
    65341 attttcttga cggcgatggc accaaacttg ctgttaattg gactgatgaa aagcgcatct
    65401 cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag tattttgctg
    65461 gtgctgctgg ttccctggct ggcttatgtg ctgtacccgc cggtactgaa gtcaggcgat
    65521 caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa
    65581 aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg cggtgattat
    65641 atcgatgccg cgatggtcgg ttacagcgtg gtggcactga tgctgcttct gcgcattatc
    65701 agttgggacg acattgtcag taataaagcc gcgtggaacg ttttcttctg gctggcctcg
    65761 cttatcaccc tcgctaccgg actcaacaac actggtttta ttagctggtt tggcaaactg
    65821 ttagcaggca gcttaagcgg ttattcgcca acgatggtga tggtggcgtt gattgtggtg
    65881 ttttatctgc tgcgctactt tttcgccagc gccacggcgt atacctccgc cctcgcaccg
    65941 atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    66001 gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg acccagtccg
    66061 atttactacg gtagtggtta tctgccaacg gtggattact ggcgactggg ggcgattttt
    66121 ggactgatat tcctcgtgtt gctggtgatt accggcttgc tgtggatgcc cgtggtgttg
    66181 ctttaagtga ggacggtgcc ggaaattgct ctccggcacc ggatagtttt acgcagccgg
    66241 taactctgcc agtggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
    66301 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    66361 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tccgccagct tcgcccgtaa
    66421 cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa
    66481 cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    66541 atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt
    66601 cagaccagaa aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa
    66661 gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag gataatccag
    66721 ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc
    66781 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggacac
    66841 cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
    66901 cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca gagaacgccc
    66961 cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    67021 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
    67081 tttgcgcacg tgatcgcggg aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa
    67141 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    67201 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    67261 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    67321 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
    67381 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
    67441 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
    67501 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
    67561 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
    67621 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
    67681 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
    67741 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
    67801 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
    67861 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
    67921 gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag aaacagtttt
    67981 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
    68041 agctcgagtt cgttgaaacg gtcgaagagc tggcagcaat gcacaatctt gaagtgccat
    68101 ttgaagcagg cagcggcccc agccagatcg agcgccatca gaggcaaacg ctttatcagt
    68161 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
    68221 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
    68281 gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
    68341 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
    68401 gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
    68461 gcgggcgcgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
    68521 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
    68581 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
    68641 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
    68701 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc gatgccgcat
    68761 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
    68821 ttttgccgga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg
    68881 aagcgcggat ggagcaggcg atgccgcttt ccgcatttct gtttaacagc ctgatgccgc
    68941 aagttgatct gagtacccct gacgggcgcg cacgtttgag tatgctggca ctgccattga
    69001 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
    69061 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
    69121 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
    69181 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
    69241 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
    69301 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
    69361 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
    69421 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
    69481 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
    69541 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
    69601 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
    69661 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
    69721 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
    69781 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
    69841 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
    69901 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    69961 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    70021 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    70081 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    70141 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    70201 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    70261 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    70321 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    70381 cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg
    70441 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
    70501 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    70561 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    70621 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
    70681 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
    70741 tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag cagattgaag aagaaaccgg
    70801 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    70861 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
    70921 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca acatcggtct
    70981 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    71041 ctggtggatc cgtcaggcga tcacccgttc tatcgcggat caggcgcgca ccatccgtat
    71101 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatgctgca
    71161 agagatgggc cgtgaaccga cgccggaaga actggctgaa cgtatgctga tgccggaaga
    71221 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    71281 tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg agctgccgct
    71341 ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac
    71401 cgcgcgtgaa gcgaaagttc tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac
    71461 gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc
    71521 gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgttgcgta gcttcctgga
    71581 cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc ggcactagtc cctttgcaca
    71641 aacgccacct tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct
    71701 cgcgatacgc ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca
    71761 gcacccaaat ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgtgct
    71821 ggctgaatcc ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat
    71881 cttcaatttt ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat
    71941 tggcttgcac cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat
    72001 gctgtgcctc ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc
    72061 agaagcgatt tgctggatga gcaaagggaa aaccagtccc agcggatgaa agcccagggt
    72121 tgataccgca aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc
    72181 ctttctaaca tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc
    72241 aggcgcacgg taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc
    72301 ccttagctca gtggttagag caggcgactc ataatcgctt ggtcgttggt tcaaacccaa
    72361 caggggccac catatctatc aaggggttac gttaatagcg tgaccccttc gttttttcta
    72421 ggatacctat aggataccgt gacagagtat tagcaggtat aaaaacaaca agttacgcaa
    72481 catcatacct gtcatgaaac tttaactatc cgtcatgata gctggtcgat gatcacggat
    72541 aatccgttat gtggatcccc atatcggcat caggcccacc agcgtgaagc gtttttcatt
    72601 tttcgcgagt ataaaaaatc gatagggctg gcagtccgga agacatcagg gtacagatat
    72661 tttacccgcc ctccccgtgc atgtgcacaa taagtaaccg aaggcggaat tccgccctcg
    72721 gtactcacat atgagaaacg atatttcttc caccgatacc ctttacttgg gtatcgtggg
    72781 taagccccaa aatctgggtg ttgctcttac tacccaaact acgggtagtt tccgtagttt
    72841 catagtcgag ttgcagatct gcaactcgcc atcagtcagg gcaattttgt aatttcacgg
    72901 gtgtaaaaat ttgacgaggc gggcggtccc ataagcaatg ggttgtagga atttcgatcc
    72961 ccctcctgaa tgcaattttt cgtatatata tacaaaacag tgcgggttag cgggttatct
    73021 tcgcgtgcta agtatttttt gttctttaat atcaacgcgt taagaaatgg tgtgtttaaa
    73081 tattagtcaa attgtaaccc gcaagtcatt caaaataacc cgcaaacacc cccaaataac
    73141 ccgcaaacgc atcaggaacg tggggccagt gattccaccg aacggctgaa cctgattgcg
    73201 ataatcctgt ttttcattgt cagtatgcag attaaaacat tcgtattgtg acggtgaaca
    73261 acgtttgccg ttaccagtcg ttacccccca tcgtttaaaa agttttgagt tgtgccctcc
    73321 gaaaaccggg acaccgggac atcgaaaacg aatgtcccat tgatgggaca tgga
//
LOCUS       DAHBHC010000015        71563 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHBHC010000015 DAHBHC010000000
VERSION     DAHBHC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.015"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(27..263)
                     /locus_tag="M2A35_001316"
     CDS             complement(27..263)
                     /locus_tag="M2A35_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCB7197636.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            404..1192
                     /gene="fhuF"
                     /locus_tag="M2A35_001317"
     CDS             404..1192
                     /gene="fhuF"
                     /locus_tag="M2A35_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCB7197637.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1230..1907)
                     /gene="bglJ"
                     /locus_tag="M2A35_001318"
     CDS             complement(1230..1907)
                     /gene="bglJ"
                     /locus_tag="M2A35_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCB7197638.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFTAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLIIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1865..2590)
                     /locus_tag="M2A35_001319"
     CDS             complement(1865..2590)
                     /locus_tag="M2A35_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCB7197639.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3209..3979
                     /locus_tag="M2A35_001320"
     CDS             3209..3979
                     /locus_tag="M2A35_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCB7197640.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3970..4443
                     /locus_tag="M2A35_001321"
     CDS             3970..4443
                     /locus_tag="M2A35_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCB7197641.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4550..5089
                     /gene="dnaT"
                     /locus_tag="M2A35_001322"
     CDS             4550..5089
                     /gene="dnaT"
                     /locus_tag="M2A35_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCB7197642.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5092..5829
                     /gene="dnaC"
                     /locus_tag="M2A35_001323"
     CDS             5092..5829
                     /gene="dnaC"
                     /locus_tag="M2A35_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCB7197643.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5878..6372
                     /locus_tag="M2A35_001324"
     CDS             5878..6372
                     /locus_tag="M2A35_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCB7197644.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6626..8917
                     /gene="opgB"
                     /locus_tag="M2A35_001325"
     CDS             6626..8917
                     /gene="opgB"
                     /locus_tag="M2A35_001325"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCB7197645.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9057..10079)
                     /gene="lgoD"
                     /locus_tag="M2A35_001326"
     CDS             complement(9057..10079)
                     /gene="lgoD"
                     /locus_tag="M2A35_001326"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCB7197646.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10218..11132
                     /locus_tag="M2A35_001327"
     CDS             10218..11132
                     /locus_tag="M2A35_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCB7197647.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11347..12708
                     /locus_tag="M2A35_001328"
     CDS             11347..12708
                     /locus_tag="M2A35_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7197648.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(12757..14421)
                     /gene="tsr"
                     /locus_tag="M2A35_001329"
     CDS             complement(12757..14421)
                     /gene="tsr"
                     /locus_tag="M2A35_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCB7197649.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(14540..14986)
                     /gene="hpaR"
                     /locus_tag="M2A35_001330"
     CDS             complement(14540..14986)
                     /gene="hpaR"
                     /locus_tag="M2A35_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCB7197650.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            15258..16547
                     /locus_tag="M2A35_001331"
     CDS             15258..16547
                     /locus_tag="M2A35_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCB7197651.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            16544..18010
                     /gene="hpaE"
                     /locus_tag="M2A35_001332"
     CDS             16544..18010
                     /gene="hpaE"
                     /locus_tag="M2A35_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCB7197652.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            18012..18863
                     /gene="hpaD"
                     /locus_tag="M2A35_001333"
     CDS             18012..18863
                     /gene="hpaD"
                     /locus_tag="M2A35_001333"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCB7197653.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            18873..19253
                     /locus_tag="M2A35_001334"
     CDS             18873..19253
                     /locus_tag="M2A35_001334"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCB7197654.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            19364..20167
                     /gene="hpaH"
                     /locus_tag="M2A35_001335"
     CDS             19364..20167
                     /gene="hpaH"
                     /locus_tag="M2A35_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCB7197655.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            20178..20966
                     /gene="hpaI"
                     /locus_tag="M2A35_001336"
     CDS             20178..20966
                     /gene="hpaI"
                     /locus_tag="M2A35_001336"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCB7197656.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            21141..22517
                     /gene="hpaX"
                     /locus_tag="M2A35_001337"
     CDS             21141..22517
                     /gene="hpaX"
                     /locus_tag="M2A35_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCB7197657.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            22527..23417
                     /gene="hpaA"
                     /locus_tag="M2A35_001338"
     CDS             22527..23417
                     /gene="hpaA"
                     /locus_tag="M2A35_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCB7197658.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            23668..25230
                     /gene="hpaB"
                     /locus_tag="M2A35_001339"
     CDS             23668..25230
                     /gene="hpaB"
                     /locus_tag="M2A35_001339"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCB7197659.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            25248..25760
                     /locus_tag="M2A35_001340"
     CDS             25248..25760
                     /locus_tag="M2A35_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCB7197660.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            26153..28303
                     /gene="btsT"
                     /locus_tag="M2A35_001341"
     CDS             26153..28303
                     /gene="btsT"
                     /locus_tag="M2A35_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCB7197661.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            28353..28556
                     /locus_tag="M2A35_001342"
     CDS             28353..28556
                     /locus_tag="M2A35_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCB7197662.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            28567..29523
                     /gene="yjiA"
                     /locus_tag="M2A35_001343"
     CDS             28567..29523
                     /gene="yjiA"
                     /locus_tag="M2A35_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCB7197663.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPSPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(29601..32141)
                     /locus_tag="M2A35_001344"
     CDS             complement(29601..32141)
                     /locus_tag="M2A35_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HCB7197664.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(32493..32657)
                     /locus_tag="M2A35_001345"
     CDS             complement(32493..32657)
                     /locus_tag="M2A35_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCB7197665.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            32834..34246
                     /locus_tag="M2A35_001346"
     CDS             32834..34246
                     /locus_tag="M2A35_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCB7197666.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(34488..35432)
                     /locus_tag="M2A35_001347"
     CDS             complement(34488..35432)
                     /locus_tag="M2A35_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCB7197667.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            35899..37131
                     /gene="mdtM"
                     /locus_tag="M2A35_001348"
     CDS             35899..37131
                     /gene="mdtM"
                     /locus_tag="M2A35_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCB7197668.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTESRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            37172..38452
                     /locus_tag="M2A35_001349"
     CDS             37172..38452
                     /locus_tag="M2A35_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCB7197669.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            38568..39719
                     /locus_tag="M2A35_001350"
     CDS             38568..39719
                     /locus_tag="M2A35_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HCB7197670.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            39729..40496
                     /locus_tag="M2A35_001351"
     CDS             39729..40496
                     /locus_tag="M2A35_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197671.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            40493..40750
                     /locus_tag="M2A35_001352"
     CDS             40493..40750
                     /locus_tag="M2A35_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCB7197672.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            40815..41675
                     /locus_tag="M2A35_001353"
     CDS             40815..41675
                     /locus_tag="M2A35_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCB7197673.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            41743..42927
                     /locus_tag="M2A35_001354"
     CDS             41743..42927
                     /locus_tag="M2A35_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7197674.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIGIALGNEYV
                     LAGLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLA
                     ILYGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGAL
                     PCLTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLN
                     LLGFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVV
                     TS"
     gene            complement(42940..43494)
                     /gene="kptA"
                     /locus_tag="M2A35_001355"
     CDS             complement(42940..43494)
                     /gene="kptA"
                     /locus_tag="M2A35_001355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCB7197675.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            43744..44427
                     /locus_tag="M2A35_001356"
     CDS             43744..44427
                     /locus_tag="M2A35_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197676.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            44424..44885
                     /locus_tag="M2A35_001357"
     CDS             44424..44885
                     /locus_tag="M2A35_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCB7197677.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLIGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            44898..46070
                     /gene="iadA"
                     /locus_tag="M2A35_001358"
     CDS             44898..46070
                     /gene="iadA"
                     /locus_tag="M2A35_001358"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCB7197678.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            46135..47046
                     /gene="hypT"
                     /locus_tag="M2A35_001359"
                     /gene_synonym="qseD"
     CDS             46135..47046
                     /gene="hypT"
                     /locus_tag="M2A35_001359"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCB7197679.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(47039..47431)
                     /gene="iraD"
                     /locus_tag="M2A35_001360"
     CDS             complement(47039..47431)
                     /gene="iraD"
                     /locus_tag="M2A35_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCB7197680.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(47428..47511)
                     /gene="idlP"
                     /locus_tag="M2A35_001361"
     CDS             complement(47428..47511)
                     /gene="idlP"
                     /locus_tag="M2A35_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCB7197681.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            48103..48933
                     /locus_tag="M2A35_001362"
     CDS             48103..48933
                     /locus_tag="M2A35_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCB7197682.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRKYERARLDKENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLSLFMKSPSYPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(49074..49847)
                     /gene="uxuR"
                     /locus_tag="M2A35_001363"
     CDS             complement(49074..49847)
                     /gene="uxuR"
                     /locus_tag="M2A35_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCB7197683.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(50062..51522)
                     /locus_tag="M2A35_001364"
     CDS             complement(50062..51522)
                     /locus_tag="M2A35_001364"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCB7197684.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(51603..52787)
                     /gene="uxuA"
                     /locus_tag="M2A35_001365"
     CDS             complement(51603..52787)
                     /gene="uxuA"
                     /locus_tag="M2A35_001365"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCB7197685.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            53127..54470
                     /gene="gntP"
                     /locus_tag="M2A35_001366"
     CDS             53127..54470
                     /gene="gntP"
                     /locus_tag="M2A35_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCB7197686.1"
                     /translation="MHVLNILWVIFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTITNIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(54713..55615)
                     /gene="fimH"
                     /locus_tag="M2A35_001367"
     CDS             complement(54713..55615)
                     /gene="fimH"
                     /locus_tag="M2A35_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCB7197687.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(55635..56138)
                     /gene="fimG"
                     /locus_tag="M2A35_001368"
     CDS             complement(55635..56138)
                     /gene="fimG"
                     /locus_tag="M2A35_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCB7197688.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(56151..56681)
                     /gene="fimF"
                     /locus_tag="M2A35_001369"
     CDS             complement(56151..56681)
                     /gene="fimF"
                     /locus_tag="M2A35_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCB7197689.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(56691..59327)
                     /gene="fimD"
                     /locus_tag="M2A35_001370"
     CDS             complement(56691..59327)
                     /gene="fimD"
                     /locus_tag="M2A35_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCB7197690.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMIQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(59394..60119)
                     /gene="fimC"
                     /locus_tag="M2A35_001371"
     CDS             complement(59394..60119)
                     /gene="fimC"
                     /locus_tag="M2A35_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCB7197691.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(60156..60695)
                     /locus_tag="M2A35_001372"
     CDS             complement(60156..60695)
                     /locus_tag="M2A35_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7197692.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(60760..61308)
                     /gene="fimA"
                     /locus_tag="M2A35_001373"
     CDS             complement(60760..61308)
                     /gene="fimA"
                     /locus_tag="M2A35_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCB7197693.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(61789..62385)
                     /gene="fimE"
                     /locus_tag="M2A35_001374"
     CDS             complement(61789..62385)
                     /gene="fimE"
                     /locus_tag="M2A35_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCB7197694.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(62863..63465)
                     /gene="fimB"
                     /locus_tag="M2A35_001375"
     CDS             complement(62863..63465)
                     /gene="fimB"
                     /locus_tag="M2A35_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCB7197695.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            64921..65637
                     /gene="nanC"
                     /locus_tag="M2A35_001376"
     CDS             64921..65637
                     /gene="nanC"
                     /locus_tag="M2A35_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCB7197696.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            65657..66763
                     /gene="nanM"
                     /locus_tag="M2A35_001377"
     CDS             65657..66763
                     /gene="nanM"
                     /locus_tag="M2A35_001377"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCB7197697.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            66828..67808
                     /locus_tag="M2A35_001378"
     CDS             66828..67808
                     /locus_tag="M2A35_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCB7197698.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWGEK"
     gene            complement(68928..69074)
                     /locus_tag="M2A35_001379"
     CDS             complement(68928..69074)
                     /locus_tag="M2A35_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197699.1"
                     /translation="MSEITASRPEVVNGDIICSTSVSHILAVQKSTQLQIDTLIRHQV
                     SDIS"
     gene            complement(69159..69356)
                     /locus_tag="M2A35_001380"
     CDS             complement(69159..69356)
                     /locus_tag="M2A35_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197700.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLRQLQLLHQNR"
     gene            complement(69376..69864)
                     /locus_tag="M2A35_001381"
     CDS             complement(69376..69864)
                     /locus_tag="M2A35_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197701.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITETDNAWLYSLSNQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLIRRYGVSLIHLDAGAECLQDLPTF
                     NW"
     gene            complement(69861..70238)
                     /locus_tag="M2A35_001382"
     CDS             complement(69861..70238)
                     /locus_tag="M2A35_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCB7197702.1"
                     /translation="MNTLPDTHVREALHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSVCTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(70328..70696)
                     /locus_tag="M2A35_001383"
     CDS             complement(70328..70696)
                     /locus_tag="M2A35_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCB7197703.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKRLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(70776..70997)
                     /locus_tag="M2A35_001384"
     CDS             complement(70776..70997)
                     /locus_tag="M2A35_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCB7197704.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(71084..71560)
                     /locus_tag="M2A35_001385"
     CDS             complement(71084..71560)
                     /locus_tag="M2A35_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCB7197705.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT        17567 a        18234 c        17694 g        18068 t
ORIGIN      
        1 tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat catcagcatg
       61 atgccagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa ctgtttgtga
      121 tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca ctgatccggc
      181 gataaatccg catacatcag ccctgcaatc agcaatcctg gcagcaacac tccccagcca
      241 ctgcccagca tacgttgcaa catggtttca tctctttcat tgataatgat aaccaatatc
      301 atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg taaacctatc
      361 gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct atcgttccgc
      421 accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg ctgggcttgc
      481 acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt ttatccgcct
      541 ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac cgaatgcgct
      601 aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa cgatgatccg
      661 cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga tggtgccacc
      721 attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg aacatttcca
      781 tgctgagttt cacgaaaccg gccgtgccgc ctgtttctgg gtcgatgtgt gtgaagataa
      841 aaacgcaaca ccacattcgc cgcagcagcg aatggaaagg ttaatcagcc aggcgctggt
      901 tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta tctggagtaa
      961 taccggttat ttgatcaact ggtatcttac tgagatgaag caactgctcg gcgaggcgac
     1021 ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg gtgaagataa
     1081 tccactctgg cgtaccgtgg tactgcgcga tggcctgctg gtgcgccgca cttgctgcca
     1141 gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat gaaagcactg
     1201 ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt acttgtttcg
     1261 caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc agaactgacg
     1321 cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt acgtttaagc
     1381 tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat ttctctctcc
     1441 gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg gtcggtcgcc
     1501 tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag cgttgacgct
     1561 ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg agcttcaata
     1621 tcatcatccg caataattaa acgccgggta cgcggaaact taatcgccag ttcagtcagg
     1681 caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat gaccgccgta
     1741 aacgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt atggagctta
     1801 taagggctac ccgcaaactt tctgaataaa ctctcaatac cgatactact catgacgcat
     1861 ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct gcggcatctc
     1921 cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc catgcataca
     1981 tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg gcgctgatag
     2041 ttttattact tttcttaagc aatgaagcta tctggttgat gccccatcct ttacctaata
     2101 gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta aaatcatgag
     2161 tttcagtcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc gtattgccgg
     2221 aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac gtcgccgtag
     2281 ggcacatgag cagttctact gcctgggaat accaggagcg tgacaccatg aaaacccagt
     2341 gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa cgtggatctt
     2401 cactttcacc ggctaaatca gcaatgacta atccggaacg acgtaattgt aataaggtaa
     2461 ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc ctcatgacct
     2521 cttttagccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
     2581 ctggcaacat atttcctcca catccactgt ctaaaaatta tgatggttta ttcaggatat
     2641 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
     2701 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
     2761 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
     2821 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
     2881 ccttcctgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
     2941 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
     3001 caataaattc attatgatga taatattcat gtgatagttt gtttgcggcg agagataatt
     3061 cgctttttat caccgagctt ctcccgataa ccaggattaa tcagcgcaat tttgatctta
     3121 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
     3181 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
     3241 gctttgtatc cagtgtggat tatttcttct gcaacatggt gcggaaagcg cgttggttga
     3301 tgagctttcc tcacgactgg gtcgggcgct gggaatggac agcgttgaaa gttctatctc
     3361 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
     3421 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
     3481 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     3541 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcctgcg cctgtttctg
     3601 taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     3661 gatgtatatc cgccagctac tggcacaacg tcatcttcat ccacagatca acttttgcct
     3721 caccgctttc gccgccacca ctatttccgg tttgcttttg caacttccca ccttcagcaa
     3781 tacacccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     3841 aaatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     3901 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     3961 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     4021 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     4081 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     4141 aatattgagt ggtcaacctt tatggcttct atgctggtcg gcaccattgg tattcaatgg
     4201 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     4261 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     4321 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     4381 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     4441 taaaattgcc tcgcttaagc acacggatga gagacagtct cctctcctcc gtgtgttact
     4501 ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta tgtcttccag
     4561 agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc aaaccgttct
     4621 ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg cgttttatgc
     4681 cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg cgcgtccggg
     4741 aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc ccgttgctgt
     4801 ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact ttatccgcct
     4861 ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac tggcctcatt
     4921 cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc aacaaaaact
     4981 ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac gagatgtgaa
     5041 tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac gatgaaaaac
     5101 gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa acctgcattc
     5161 aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg ctccgccgct
     5221 ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc cggtattcgt
     5281 ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg gcagatgaat
     5341 gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc cagcttcatc
     5401 ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg caacgagctg
     5461 ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc ggcgatgaaa
     5521 gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct gagcaacgtt
     5581 gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga aaaagtgatc
     5641 atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat gctgaccaac
     5701 agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg tatgcgcctg
     5761 ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgtgt aacaggtaaa
     5821 gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg agtaatgatg
     5881 atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt aatttccacc
     5941 ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga acttggcaac
     6001 caaaacagta cgacacagga aggcggttgg tcgctcgcgt cattaactaa cttgcttagc
     6061 agcggaaacc aggccttaag cgcagataac atgaacaatg ccgcaggcat tctgcaatac
     6121 tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa ccagatgctg
     6181 gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct ggacggcatt
     6241 cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat cggaacgact
     6301 ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca ggggctgaac
     6361 ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac gcggctctct
     6421 cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag cgaccgcgat
     6481 caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga cactagaatt
     6541 gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg ttggctaaac
     6601 ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc tttttctcgc
     6661 ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg cagccacatt
     6721 aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg actattttac
     6781 tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga ccggtgctgg
     6841 cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag cggtgttcgg
     6901 tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg gttacagcct
     6961 gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccagcatttc gtcagataac
     7021 ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt attataaaga
     7081 gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg gcgaaagtct
     7141 cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac tgggcgcgtt
     7201 gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg attacacgat
     7261 tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg aaggcaacgc
     7321 ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa
     7381 ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg gtaaagatgt
     7441 gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga aaagcgtggt
     7501 ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc tcgatgaagc
     7561 gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt ttaccctgac
     7621 agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca aaaaatatga
     7681 ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg agaatatcgc
     7741 ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca tcgtcgtctc
     7801 ttctgaccat ttagcgatga acaacacggc gtggaaatac cttaataagc aggatcgcaa
     7861 taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg cagtgaagcg
     7921 taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata actatctcgg
     7981 acttggtcgt agcagtttat ccgggcagtc aatgtcggaa atcttcctca atatcaaaga
     8041 gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta aagagatgaa
     8101 agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc atttccgttt
     8161 gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc
     8221 agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg tctgggttga
     8281 ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca ccgactggtg
     8341 tgtctcgcaa gggcagcttg gcggtcagca aattgttcag catgttgaca aaacaacatg
     8401 gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata aaggcaatgt
     8461 cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt tcatcttcaa
     8521 cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc cagagtcgtg
     8581 gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca agcatccgct
     8641 gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg ccagccgtcc
     8701 tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg aagtgaccac
     8761 caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg tgccgccgga
     8821 acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg
     8881 catggtggaa attaaagttg tggaacgtga agggtaattt ctgactgatg tgtaggtcgc
     8941 ataaggtgcg acctacacat cttctccggc ctacaaaaca tcattgagaa acagaaaaaa
     9001 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     9061 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     9121 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cggcagtgat ttttccttcc
     9181 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct gcccatcatc
     9241 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     9301 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     9361 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     9421 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     9481 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     9541 gccgccgcgc ccaaaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     9601 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgcggccgcc
     9661 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     9721 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     9781 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     9841 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     9901 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     9961 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
    10021 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
    10081 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
    10141 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
    10201 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
    10261 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
    10321 cggaaatgta caacatcagc cgcacaacag tgcgtcatat tctcagccac ttacgcgaat
    10381 gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
    10441 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
    10501 ttttcacgat gataaaccag cgtcagttac gccctgggga aactttttct gaactgcaac
    10561 tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta aaattcggcc
    10621 gttataatct tattcaaagc gaaaaacgcg gccagtggag catgaagcaa ttcgatcaat
    10681 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
    10741 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
    10801 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
    10861 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
    10921 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
    10981 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
    11041 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatcc atgattcgat
    11101 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
    11161 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
    11221 atcagtcgac taaaaatttt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
    11281 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
    11341 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
    11401 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
    11461 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
    11521 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
    11581 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
    11641 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
    11701 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
    11761 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
    11821 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
    11881 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
    11941 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
    12001 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
    12061 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
    12121 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
    12181 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
    12241 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
    12301 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
    12361 atgttctgtt ctgccgcctt tacgctgata gtaccacaag cgacaacatc catgacggcg
    12421 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
    12481 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
    12541 gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc
    12601 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
    12661 tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc
    12721 cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc
    12781 gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga
    12841 tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccacag cttcggtcag
    12901 gcgactggcc tgctcttcca gcgcggcggc ggcagcggca gattcttcca ccagcgcggc
    12961 gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat cgataccacg
    13021 gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc gggtcacggc
    13081 gctgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc caacatccac
    13141 tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctgag cgctacgctg
    13201 ggccagatta cgcacttctc ccgcaaccac cgcaaaaccg cgcccttgct cacccgcacg
    13261 cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa tgccgtcaat
    13321 tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca tagtctgcac
    13381 cacgttatct accactttgc cgccccgttg cgccgtttca gaagcactta atgccagatg
    13441 gctggcctgg cgcgcattct cggcgttctg tttcaccgtt gcggtcagtt gctccatgct
    13501 ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga gatcgttatt
    13561 gccggtagcg atttcgctgg caccgctata gatggcattg gccccgttgc gcacatcacc
    13621 gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt gccccatctc
    13681 attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat gacgaatgct
    13741 gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc agacggcgaa
    13801 gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct ggctgtagga
    13861 ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca tgtaagccac
    13921 atactgcttc tcgaaaccgt cctgatatcc ctgagtcggc tgatcaaaga actcgttgat
    13981 tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa tatcgtaatt
    14041 acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca acgcttcgta
    14101 atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca tcagctcagc
    14161 aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac ccgcgcggtt
    14221 gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg attgctgctg
    14281 gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat taaagaacag
    14341 accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta agctggtcac
    14401 gatcttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaag attttcggcc
    14461 tggaaagcaa aaactttatg aaaaggtgta acccgccatc catggaaaaa ccaggcgctg
    14521 gttatcagat actaaaaagt tattcattat cgcccgggat gtcttcttgt cgggaattgc
    14581 ccagagcaat aaattcctct aacaaatggg ttaactgttg cattttctcg gcagtgaact
    14641 gagcttcaat ctgccgatag gcttcttcaa tctgtgtctg agcgcggtta tacagcgcct
    14701 gcccctcttt ggtcagcgag atatagagct tccgctgatc attgatcggc tttaatcgca
    14761 acactaaacc gtcgcgttcc atccgcgtaa ggatcccggt caggcttggg cgcaaaatgc
    14821 aggcgcgata cgccagatcg tgaaaatcca ttgatgggct ctccgccaga atacgcacga
    14881 tgcgccactg ttgctcagtc aaattatgcc gtttaacgat tgggcgaaaa taactcatcg
    14941 ccgcttctcg cgcctggagc aacgcaatgg ttagagagtc gtgcattatc tttcccctga
    15001 aaaattcaaa atcattaata tagaaacagt taatcatcac gttaccggga ctgcgctgcc
    15061 cctgcaattc ttcattttat caaaattgag catgttttgt taacttgctg atatttaata
    15121 ttttattatc tttaaatgta aatagtttgt taattagatc acatttacat cacttacttt
    15181 tgccaaacta cgcaatcggg aataaaagta atcattaaca tattaatgat taagatgatc
    15241 gaatccgagg agtggtaatg aaaggcacta tcttcgccgt agcattgaac catcgcagcc
    15301 agcttgatgc atggcaggaa gcgttccagc aatcccccta caaagccccg cctaaaactg
    15361 cggtctggtt tattaaaccg cgcaatacgg tgattggttg cggtgaaccg attccctttc
    15421 cacagggtga aaaggtactg agcggtgcga cagttgcgct gattgtggga aaaacggcga
    15481 cgaaagtacg tgaagaagat gcggcagagt acatcgccgg atatgcgctg gctaacgacg
    15541 tcagcctgcc ggaagagagc ttttaccgtc cggcaatcaa agcaaaatgt cgtgatggat
    15601 tctgccccat tggcgaaacc gtggctctca gcaatgtcga taatctgacc atctataccg
    15661 agatcaacgg gcgtcctgcc gatcactgga acaccgccga tttacaacgt aacgccgcgc
    15721 agttgctgag cgccctgagc gaatttgcca cactgaatcc aggcgatgca attctgctcg
    15781 gcacgccaca ggcgcgcgtg gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag
    15841 gtttcccgcc gctggaaaat ccggtagtgg acgaacgtga agtgaccacg cgcaagagct
    15901 tcccaacgca gccacacccg cacggcacgc tgtttgccct cggcctgaac tacgccgacc
    15961 acgccagcga actggaattt aagccaccgg aagaaccgct ggtgttcctg aaagcgccaa
    16021 ataccctcac tggcgataac cagacctccg tgcgtccaaa caatattgaa tacatgcact
    16081 atgaagcgga gctggtggta gttattggca aacaggcgcg tagcgtcagc gaagccgatg
    16141 ccatggatta tgtcgcgggc tacaccgtgt gtaacgacta cgccattcgc gactatctgg
    16201 aaaactacta ccgccctaac ctgcgggtaa aaagccgcga cggactgacg ccgatgcttt
    16261 caaccatcgt gccgaaagag gcgatcccgg acccgcataa tctgaccctt cgcaccttcg
    16321 tcaacggcga gttacgccag caaggcacca ccgccgatct gatcttcagc gtgcccttcc
    16381 tgatcgccta cttaagcgaa tttatgaccc tgaatccggg cgacatgatc gccaccggca
    16441 caccaaaagg cttatctgac gtggtgcctg gcgatgaagt agtggtggaa gtagaaggcg
    16501 tgggccgcct ggtgaaccga attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca
    16561 ttggatcaac ggcaaaaatg ttgcaggtaa cgactacttc cagaccacca atccggcaac
    16621 gggtgaagtg ctggcggatg tggcctctgg cggtgaagcg gagatcaatc aggcggtagc
    16681 ggcagcgaaa gaggcgttcc cgaaatgggc caatctgcca atgaaagagc gtgcgcgcct
    16741 gatgcgccgt ctgggcgatc tgatcgacca gaacgtgcca gagatcgccg cgatggaaac
    16801 cgcggacacc ggcctgccga tccatcagac caaaaatgtg ttgatcccac gcgcttccca
    16861 caactttgaa tttttcgcgg aagtctgcca gcagatgaac ggcaagactt atccggtcga
    16921 cgacaagatg ctcaactaca cgctggtgca gccggtaggc gtttgtgcac tggtgtcacc
    16981 gtggaacgtg ccgtttatga ccgccacctg gaaggtcgcg ccgtgtctgg cgctgggcaa
    17041 taccgcggta ctgaaaatgt cggaactctc cccgctgacc gccgaccgcc tgggtgagct
    17101 ggcgctggaa gctggtattc cggcgggcgt gctgaacgtg gtacagggct acggcgcaac
    17161 cgcaggcgat gcgctggtcc gtcatcatga cgtgcgtgcc gtgtcgttca ccggcggtac
    17221 ggcgaccggg cgcaacatca tgaaaaacgc cgggctgaaa aaatactcca tggaactggg
    17281 cggtaaatcg ccggtgctga tttttgaaga tgctgatatt gaacgcgcgc tggacgccgc
    17341 cctgttcacc atcttctcga tcaacggcga acgctgcacc gccggttcgc gcatctttat
    17401 tcagcaaagc atctacccgg aattcgttaa acgctttgcc gaacgcgcca accgtctgcg
    17461 cgtgggcgat ccgaacgatc cgaataccca ggttggcgcg cttatcagcc agcagcactg
    17521 ggaaaaagtc tccggctata tccgtctcgg cattgaagaa ggcgcaaccc tgctggcggg
    17581 cggcccggat aaaccgtccg acctgcctgc gcacctgaaa ggcggcaact tcctgcgccc
    17641 aaccgtgctg gcagacgttg ataaccgtat gcgagtcgcc caggaagaga ttttcgggcc
    17701 ggtcgcctgc ctgctgccgt ttaaagacga agccgaaggc ttacgtctgg caaacgacgt
    17761 ggagtacggc ctcgcgtcgt acatctggac acaggatgtc agcaaagtgt tacgcctggc
    17821 gcgtggcatt gaagctggca tggtgttcgt caacacccag aacgtgcgtg acctgcgcca
    17881 gccatttggc ggcgtaaaag cctccggcac cgggcgtgaa ggcggtgagt acagcttcga
    17941 agtgttcgcg gaaatgaaga acgtctgcat ttccatgggc gaccatccaa ttccgaaatg
    18001 gggagtctga tatgggtaag ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt
    18061 atctctctga actgccaggg aaaaaccacg gttgccgcca gggcgcgatc gacgggcata
    18121 aagagatcag caagcgttgc cgggaaatgg gcgtcgatac cattatcgtt ttcgataccc
    18181 actggctggt caacagtgct tatcacatca actgtgcaga ccattttgaa ggcgtctaca
    18241 ccagcaacga gctgccgcat tttatccgcg atatgaccta caactacgag ggcaacccgg
    18301 agttggggca gcttattgcc gatgaagcct taaagctcgg cgtgcgggca aaagcgcaca
    18361 acattcccag cctgaaactg gaatacggca cgctggtgcc aatgcgctac atgaatgaag
    18421 acaagcactt caaagtggtt tccatttcag ctttctgcac tgttcacgat tttgccgaca
    18481 gccgcaagct gggcgaggcg attctgaaag cgatcgaaca gtacgacggc accgtggcgg
    18541 tccttgccag cggttcgtta tcgcaccgct ttattgacga tcagcgagcg gaagaaggga
    18601 tgaacagcta cacccgcgag ttcgaccgcc agatggacga gcgtgtggtg aagctgtggc
    18661 gcgaaggcca gttcaaagag ttctgcaata tgctgccgga gtacgccgac tactgctacg
    18721 gcgaaggcaa tatgcacgac acggtgatgc tgctggggat gctcggctgg gataaatacg
    18781 acggcaaggt ggagtttatc accgagctat tcccaagctc tggcaccggt caggttaacg
    18841 ctgttttccc gcttcccgcg taaggacgtt ttatgccgca ctttatcgtt gaatgcagtg
    18901 acaacatccg cgaagaagcc gacctgccgg ggttgttcgc caaagtgaat ccgacgctgg
    18961 cagccacggg tatttttccg ctggcgggta ttcgcagccg cgtgcattgg gtcgatacct
    19021 ggcagatggc cgacgggcag cgtgattatg ccttcgtgca tatgacgttg aaaatcggcg
    19081 caggtcgcag cctggaaagc cgtcagcagg cgggtgaaat gctgtttgaa ctgattaaaa
    19141 cgcacttcgc cgccctgatg gagagccgcc tgctggcgtt gtcgtttgag attgaagagc
    19201 tgcatccgac gctgaatttt aaacaaaaca acgtgcacgc attgtttaag tagcgcgcag
    19261 attgcccggt ggcgctgcgc ttaccgggcc tacaaaaccc caaaccgtac accgtaggcc
    19321 ggataaggcg cagccgcatc cggcaatgcc acaggatatc gctatgttcg ataaacatac
    19381 ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc
    19441 gatctcactg gattacccgg agatcaccat cgaagacgct tacgcggtgc agcgcgaatg
    19501 ggttcgcctg aaaatcgccg aaggtcgcac gctgaaaggc cacaaaatcg gcctgacctc
    19561 gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggcgcgc tgctggatga
    19621 catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga
    19681 agtggagctg gctttcgtgc tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga
    19741 cgtctacaac gccacggact acgtgatccc ggcgctggag ctgatcgacg ctcgctgcca
    19801 caacatcgat ccggaaaccc agcgcccgcg caaagtgttc gacaccattt ctgataacgc
    19861 cgccaacgcc ggggtgatcc tcggtggtcg tcccattaag cccgatgagt tggatctgcg
    19921 ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg
    19981 tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg caccgtatga
    20041 cgtacaactg gaagccgggc aaatcattct cggcggttcg ttcacccgcc cggttccggc
    20101 gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta gctgccgctt
    20161 tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca ggccgcccgc
    20221 agatcggatt atggctgggg ctgagtagca gctacagcgc agagttactg gccggagcag
    20281 gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa accgtgctca
    20341 cccagctaca ggcgattgcg ccctacccca gccagccggt ggtacgtccg tcgtggaacg
    20401 atccggtgca aatcaaacaa ctgctggacg tcggcacaca aaccttgctg gtgccgatgg
    20461 tacaaaacgc tgacgaagcc cgtgaagcgg tacgcgccac ccgttatccc cccgccggta
    20521 ttcgcggtgt gggcagtgcg ctggcccgag cctcgcgctg gaatcgtatt cctgattacc
    20581 tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga
    20641 agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc ggcccggcgg
    20701 atctgagcgc cgatatgggt tatgccggta atccacagca cccggaagta caggccgcga
    20761 ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc ctgatcgcca
    20821 atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgct gtcggcgttg
    20881 acaccaccct gctcgcccgc gccgctgaag cgctggcagc acggtttggc gcacaggcca
    20941 cgaccgttaa gcccggcgtg tattaatgcc ggatgcgctg cacttatccg gcctacactc
    21001 gcaccaaacc gtaggccgga taaggcgttt acgccgcatc cggcaaaaag ccgtaccaaa
    21061 tcgcggaccg gataaggcgt ttgtgcgcat ccgccataaa ccttgtcgta ccctacaaaa
    21121 atcccattag aggaagaaaa atgagcgaca cctcacctgc cataccggag agtatcgatc
    21181 cggcgaatca gcataaagcg ctgactgccg gacaacaggc ggttattaag aagctgtttc
    21241 gccgcctgat cgtctttctg ttcgtgctgt ttatcttctc gttccttgat cgcatcaaca
    21301 tcggctttgc cggactcacg atgggacgcg acctcggtct gagcgccacc atgtttggcc
    21361 tcgctaccac cctgttctat gccgcttatg tcatcttcgg cattcccagc aacattatgc
    21421 tgagtattgt cggtgcgcgg cgctggatcg ccaccatcat ggtgctttgg ggcatcgcct
    21481 ctactgccac catgtttgcc actggcccca ccagcttgta cgtactgcgt atactggttg
    21541 gcattaccga agccggcttt ctgcccggca ttctgctgta tttaaccttc tggtttccgg
    21601 cctatttccg cgcccgtgcc aacgccttgt ttatggtagc aatgccggta acgacagcgt
    21661 tgggctcgat cgtttccggc tacattttgt cgctggatgg cgtcatggca ttaaaaggct
    21721 ggcagtggct gtttttgctg gaaggcttcc cgtcggtatt actcggcgtc atggtgtggt
    21781 tctggcttga tgactcaccg gacaaagcta agtggctgac gaaagaagac aaaaaatgcc
    21841 tgcaagagat gatggataac gatcgtctga cgctggttca gccagaggga gccatcagcc
    21901 accacgccat gcaacaacgc agtatgtggc gggagatctt cactccggtg gtgatgatgt
    21961 ataccctggc gtatttctgc ctgaccaaca cacttagtgc gatcagcatc tggacaccgc
    22021 agatcctgca aagttttaat cagggcagca gtaatatcac catcggcctg ctggccgccg
    22081 taccgcagat ttgtaccatt ctcgggatgg tctactggag ccgtcactca gatcgccgcc
    22141 aggaacgaag gcatcacacc gcccttcctt atttgttcgc tgccgcaggt tggttactgg
    22201 cttcggcaac tgatcacaac atgatccaga tgctggggat cattatggct tcgaccggat
    22261 cattcagcgc aatggcgatt ttctggacaa caccggatca gtccatcagc ctgcgggcac
    22321 gagcgatcgg tattgcggtg atcaacgcca ctggcaacat tggttcagcg ttaagtccgt
    22381 ttatgatcgg ctggttgaaa gatttgaccg gcagctttaa cagtggattg tggtttgttg
    22441 ccgcgctgct ggtgattggt gcggggatta tctgggcgat tccaatgcag tcctcccgtc
    22501 cgcgagcgac cccgtaagga acgacgatgt gtgaccgtca gattgccaat attgatatca
    22561 gcaaagagta cgatgaaagc ctgggcacgg acgatgtgca ttatcagtcc ttcgcccgca
    22621 tggcggcctt ttttggccgc catatgctgc cacatcgcca cgaacagtac tttcagatgc
    22681 atttcctcaa tagcggacag attgagctac aacttgacga tcatcgctac tcggtggaag
    22741 cgcccctgtt tgtcctgacg ccgccgtcag tacctcatgc gtttattacg gagtctgacg
    22801 ccgacggtca tgtgttgacg gtacgggaag atctgatctg gcccctgctg gaagttcttt
    22861 atccgggcac tagggaaacc ttcggcctgc cggggatttg cctgtcactg gcagataaac
    22921 ccgacgaact ggcggcgctg gaacactatt ggcaactgat agagcgggaa tcggtggaac
    22981 aactgcctgg acgggaacac accctgacgt tactggcaca ggcagtgttc accctactgc
    23041 tgcgtaacgc aaaactcgac gaccatgccg ccagcgggat gcgcggagaa ttaaaactgt
    23101 tccagcgttt taatatgctg attgaaagcc attttcatca gcactggaca gtaccggatt
    23161 acgctaacga actgcatatc accgaatcac gactcacgga catctgccgt cgctttgcca
    23221 accgtccgcc aaaacggttg attttcgaca ggcagctgcg agaagccaag cggctgctgc
    23281 tgttttctga taacgccgtg aacaatattg cctggcaact cggttttaag gatccggctt
    23341 attttgcgcg cttttttaat cgcttagtcg gttgctcgcc cagtgcttat cgcgccaaaa
    23401 aagtacctgt gacgtgaaaa tcccttagcc ttaacgggaa accaggcacc acctgctatt
    23461 cccctttctc cgcctggagc aaggggattt agtaacgtca aaaatcgcta aaagcgacct
    23521 cgatcacaaa accgtacccg acctgaaaag taccagccat tgtcccaaaa gtctcttccc
    23581 gtaatcgccc ctgcaagctt caattgaata acaaaaacaa cacacaaaat taacaatcgt
    23641 attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa
    23701 cgtccgttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc
    23761 tatggcgagc gagtgaaaga cgtcactact catccggcat ttcgtaatgc ggcagcgtct
    23821 gttgcccaac tgtacgacgc gctacacaaa ccggagatgc aggactctct gtgctggaac
    23881 accgacaccg gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac
    23941 gacctgcgcc agcaacgcga tgccatcgct gagtggtcac gcctgagcta tggctggatg
    24001 ggccgtaccc cagactacaa agctgctttc ggttgcgcac tgggcgcgaa tccgggcttt
    24061 tacggtcagt tcgagcagaa cgcccgtaac tggtacaccc gtattcagga aactggcctc
    24121 tactttaacc acgcgattgt taacccaccg atcgatcgtc atttgccgac cgataaagtg
    24181 aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt tagcggtgcg
    24241 aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca
    24301 caagtaatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat ggatgccaat
    24361 ggcgtcaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac cggctcgccg
    24421 tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt gatggataac
    24481 gtgctgatcc catgggaaaa cgtactgatc taccgcgatt tcgatcgctg ccgtcgctgg
    24541 acgatggaag gcggttttgc ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg
    24601 aaactagact tcattacggc actgctgaaa aaatcactcg aatgtaccgg caccctggag
    24661 ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca
    24721 ttgagtgact cgatgtgttc tgaagcgacg ccgtgggtca acggggctta tttaccggat
    24781 catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac
    24841 attatcgaac gcaacgttac cagtggcctg atctatctcc cttccagtgc ccgtgacctg
    24901 aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa tggtatggat
    24961 cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag cgagtttggt
    25021 ggtcgccacg aactgtatga aatcaactac tccggtagcc aggatgagat tcgcctgcag
    25081 tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat ggttgatcgc
    25141 tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa caacgacgat
    25201 atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg caattagatg
    25261 aacagcgcct gcgctttcgt gacgcgatgg ccagcctgtc ggcagcggta aatattatca
    25321 ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggccgtctgc tcggtcacgg
    25381 atacaccacc atcgctgatg gtgtgcatta acgccaacag tgcgatgaac ccggtttttc
    25441 agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg atggcacgcc
    25501 acttcgcggg catgacgggc atggcgatgg aagagcgttt tagcctctca tgctggcaaa
    25561 aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa ggtgagatcc
    25621 gcgatgtgca ggcaattggc acacatctgg tgtatctggt ggagattaaa aacatcatcc
    25681 tcagtgcaga aggtcacgga cttatctact ttaaacgccg tttccatccg gtgatgctgg
    25741 aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg ggcattttta
    25801 ttgtgatctc aatcacatat ccacccgatg aataattctt gcccaaccgc tcaccgccat
    25861 tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt tacgcgcgcg
    25921 taactctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa
    25981 cctagaacgg cttcggccta tgattaacaa attactctat gaattttggt ttgcaggaag
    26041 gcagcaagtg agtgaatccc cgggagctta caacagtaag tgacaggggt gaacgaacgc
    26101 aactgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta ctatggatac
    26161 gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg cattctgcct
    26221 cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc
    26281 tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc agaaggtgat
    26341 gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc tgaactacgt
    26401 tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc
    26461 gctggtcggt ccggttctcg ccgcacagat gggctacctg cctggcacgc tgtggctgct
    26521 ggcgggggta gtactggccg gtgcggttca ggactttatg gtgctgttta tctcctctcg
    26581 ccgtaacggc gcatctctcg gtgagatgat caaagaagag atgggaccag taccggggac
    26641 tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgtcc tggcgctgat
    26701 tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct caaccgtacc
    26761 gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt
    26821 ctctgtcatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg tgattgctca
    26881 cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct tcgcgctgat
    26941 tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta
    27001 tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct
    27061 gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg gcccgctgtg
    27121 gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg tatctggctt
    27181 ccacgcgctg atctcttccg gtacgacgcc aaaactgctg gctaacgaaa ccgacgcgcg
    27241 tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg cgctggttgc
    27301 agcgtccatc atcgaaccgg gtctttactt cgcgatgaat accccgcctg ctggccttgg
    27361 catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca tcatggcgca
    27421 gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct tcgtgatttc
    27481 gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc
    27541 aggtggtgcg ccaacgctgg cggtaggtat cgctcacgtg ttccacaaag tgctgccgat
    27601 ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt tcatcctgac
    27661 cgcgctggat gcgggtactc gttctggccg ctttatgctg caagacctgc tgggtaactt
    27721 catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta ctgcgggctg
    27781 tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa
    27841 gagcctgtgg ccgctgttcg gtatttccaa ccagatgctg gcagccgtag cgctggtact
    27901 gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa ctgttgttcc
    27961 ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa
    28021 cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga ttgctaacgg
    28081 tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga acaactacac
    28141 caacgcgggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct tctacggttt
    28201 caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa caccgtacgt
    28261 tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtatt tagccccgct
    28321 tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga caggcaaaaa
    28381 aatatctcgg ccaggcggcg aagatgttga ttggcattcc agactatgac aactacgtcg
    28441 agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg
    28501 aacgccagaa cgcacgctac ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga
    28561 gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc gggcaaaacc
    28621 accctgctgc gccatattct taatgaacaa cacggctaca agattgccgt gattgaaaac
    28681 gaattcggcg aagtctccgt tgatgatcaa ttgattggtg atcgcgcaac gcagatcaaa
    28741 acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga cgcgctactc
    28801 gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt cattgaatgc
    28861 accggcatgg ccgatcctag cccgattatt caaacctttt tctcccatga gattttatgc
    28921 cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc cgatgagcag
    28981 atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat tctgctgact
    29041 aaaaccgacg tcgcaggcga agcagaaaaa ctgcgtgaac gcctggcgcg catcaacgcc
    29101 cgtgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct gttcaacacc
    29161 aacggtttta tgctggaaga aaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg
    29221 gataaacaaa acgatatttc gtcgattgtg gtggaactgg attacccggt agatatcagc
    29281 gaagtttccc gggtgatgga aaacctgctg ctggaatcgg cggataaact gctgcgttac
    29341 aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc
    29401 ctctacagcg ccgactggga caggccatgg ggcgatgaaa caccgcatag cacgatggtg
    29461 tttatcggga ttcagttgcc ggaggatgag attcgggcgg cgtttgcggg actcaaaaag
    29521 taaccagccc tccaacatca caatacctga gactttatca ccctcatatt cctccagaat
    29581 atgagggctt tcgggcttaa ttatctctga acgtgcaaca cttcctgcga ctccttatgc
    29641 gcctccacat cctgcttcgt taaccagagc gacttacggc caaaattttc gtacattttc
    29701 agctgatctt catagtgctt atcaactgtt ccatcgggag caataaaccc actctgaccg
    29761 ggtgcgacca catcccaggc aagcacagga cgatcgcttg tcgttggtga gaaaacaatc
    29821 atatcgtttt ctgttccacg gttttgatac tccgcctgat gacgcgtttc ttccgctgcg
    29881 gcctgcggta caccaaagaa attatttgcc cggaacgtta aggccattgc aggtgttttc
    29941 cagttactca cattattgcc atagcgtttg gaaagagtct cccaggtatc ttccagcgca
    30001 gccaacacaa cctcctgctg tggtttccca gcaaacagat caaccgcctg tgggattggt
    30061 gatttgtctc cctgcaccgc ctcatacaaa atttttgctc caacacttat attcagcgaa
    30121 ccagttgggc cgtcctgggt tgtttcgtag ccactggcgc tgtaccactt atcaaatggc
    30181 ataggtacgg cagccactac ggtacgcttc aacatactgg tcagccaaac gttcaggatg
    30241 gcagagcctg gctgctgcca ggttttacca tcatcattaa gcaaattgat gccatcccaa
    30301 cgtgttaatg tttctaccaa ctgacgacgc ggatcgctct gtgtcaaacc agatgtcgct
    30361 gcttgcagag taggtaaaaa aagcctcagg ttaagatcct gacgactggt ttggcgaata
    30421 acatcccatg cctgatcagc agttaagcgt ggcttttgct caagcagtcg gtcgatctcc
    30481 gtaacgcgat ctgcaccacc ccacaaaaag gcaaacagat ctgaagcggg ataatctttt
    30541 tggggagaat tgttccagtt agcaatatat cccgactggg ggttatacac cttagggttc
    30601 atttcaaaag gcaatagccc tttccagtcc cattttcccg taccaggaac gggtaatcgc
    30661 ggatcatggc ctgattgacg atctggataa gcaccagtat gaacataacc aatattgccg
    30721 tttacatcag catagtacca gttgatggtc agtgcttgtt tcgctgcctg ctgtgtccac
    30781 tcctgccaat ttttggcctt catctgatga gtccaggcca gcaaagacgc cacctcttta
    30841 ccatcccatg cgcgggattt agcgtaagcc gtttgtgtcg tctggtcagt ttggagaatg
    30901 ttgccatgca ccgtacgcca gacagtaaag gtctctgcct gaccattttt caccgtaatg
    30961 gtttcctcac ggcttaacat tttcacccac ttaccattat gcaagtagta gcctggtttc
    31021 tctgccgaca gccgttcagc aaaaatatcg acatcatcgc cgaaacctgc cgttgatccc
    31081 caggaaatca caccattatg accaaaaacc agcccaggat aggcaaatgg tgtattgcca
    31141 gtgacatcat aaccagcacc gtgcagacca ataccataag tatacgcagg cgcataccag
    31201 ccaaactgcg gaccatttac catgattgct ttcgcatcct gggctttgct tttgccgatc
    31261 acccacatat tgctggtcgt tggatacccc gccagaccat tggcaccacc ctgtgcaaat
    31321 tgtgcagcaa tagtttcccg gttcttccct gctgttaacg ccagcagtgc gccatccgcc
    31381 ccttttgctg gtcggtcaag cattggtgca ggtaaatcgt agcgtggcaa cagagctgct
    31441 gtttgcgagt tttgctgatt aaatttaagt gggtagttac tctcttgtac ggcaatagtg
    31501 gttggcgctg atgggtttac cagccatttc aactgattaa ataccgccat gccttgtgat
    31561 acaccatatt tatcttttaa agccgttagc agtgccagat tatcaatttc gctagtgcta
    31621 tcagagaagc ggtttgccat ggtgcccaca aatatcatcg cgacatcaaa cggttcccag
    31681 cgcttaggag taaagccaaa tgtattaaac tgttttggta agagcgtctc tggattggta
    31741 tttaccttat caatccaggc attcattcca tcagcgtagc cttgcagaat ggacatatcc
    31801 tctggggaaa gggcagcaat ttgcgcccgg atagcatccg gccagtagtt acgacggata
    31861 tctttatcaa atttcacaaa atctttgcca agcacttccg cgacagtccc ttgagtactg
    31921 cgacgtgcca tttccatctg aaaaaggcga tcttgtgcta ctacatagcc atagccataa
    31981 aataggtgcc atgtatcatt ggcataaata tgcggcatgc cgtattcatc gcgaacaatc
    32041 tttatctcac ttgacgactg ctcagccagt gcaggtaagc tccaataata catcagggaa
    32101 gcagtaacac agttcacgat catacgattt ctatttttca ttgtatcctc tggcaggtgt
    32161 ataattagcg aattgatact agcaacgaag cttcggttat ctatttgcag agaattgttt
    32221 cattatgaac tgtgagcgcg ataactaatt tttgatacaa aaatttcatt aatgtgatct
    32281 aaaacgaatc gctttattca ccgctgatga ttttgttcta ttcctttttg tgacagatcc
    32341 cccggacgca tagatgatga cccccagaga gacagctcag gtcattatct gtatgtaaat
    32401 cagaaaaata tgttgtaaac gacttttatg gttggatttt gtagcaattt tgaatgcaga
    32461 caggcagcat ttgccaacac gccgcccata gttcactcca catcctccct gcgtaaccca
    32521 atatccctca accgttcatc actcatctgc tgtaacactc gtcgggtctg catttgccgc
    32581 cgccaacgcc tcaccgcatg ccagagttgc acaagtctga taaacggcgt tttggctttg
    32641 ttttcgtgaa attccatctg ctgcctcctc atctttcaga ggcttcatca tccgccttct
    32701 ggcagttcac aatacagatg caaatattag ttttatttaa catacagaca ggttaaaaca
    32761 gcatctgcat ccggtttttt agcctcatct gtaccggttt ttctttctgt atgggccaaa
    32821 taaggaatac agcatgacgc gttatcaaca tctggcgact ctacttgccg agcggattga
    32881 gcaagggctg tatcgtcacg gggagaaatt gccgtcggtg cgcagcttaa gtcaggagca
    32941 cggcgtcagc atcagcaccg tgcaacaggc gtatcagacg ctggaaacga tgaggctcat
    33001 cactccgcag ccgcgttcgg gttattttgt cgcacaacgt aaagcccagc cgcctgtacc
    33061 gccgatgacg cgtccggtgc agcgcccggt ggaaattacc cagtgggatc aggtgctgga
    33121 tatgctggag gcgcatagcg acaattccat tgttccgtta agcaaaagca cgccggatgt
    33181 cgaaacgccc agcctgaaac cgctgtggcg tgagctaagc cgggtggtgc agcataatct
    33241 gcaaaccgtt ctcggttatg acttgttagc cggtcagcga gtgttgcggg agcagattgc
    33301 ccgcctgatg ctcgacagcg gctcggtggt caccgccgat gacatcatca tcaccagcgg
    33361 ctgccataac tcgatgtcgc tggcgttaat ggcggtgtgt aaaccgggcg atattgtcgc
    33421 ggtcgaatct ccctgttatt acggttcgat gcagatgctg cgaggcatgg gcgtgaaagt
    33481 gattgaaatc ccaaccgatc cagaaactgg catcagcgtt gaagcactgg aactggcgct
    33541 ggaacagtgg ccgattaaag gcatcattct ggtgccaaac tgtaataatc cgctgggatt
    33601 tattatgccg gacgcacgca aacgggccgt tctctctctc gctcagcgtc atgatattgt
    33661 gatttttgaa gatgatgtct acggcgaact ggcaacggag tatccgcgcc cgcggaccat
    33721 ccattcatgg gatatcgacg ggcgagtgct gttgtgcagc tcgttcagta aaagtattgc
    33781 tccaggcctg cgcgtgggtt gggtcgcacc ggggcgttat cacgataaac tgatgcatat
    33841 gaaatacgcc atcagcagct ttaatgtgcc gtccacgcaa atggcggcgg caacgtttgt
    33901 gttggaaggc cactatcatc gccatatccg gcggatgcgg cagacttatc agcgcaatct
    33961 ggcgctttat acctgctgga tacgggaata ttttccctgc gaaatctgta ttacgcgccc
    34021 gaaaggcgga tttttactgt ggatagaatt gcctgaacag gtcgatatgg tctgcgttgc
    34081 gcggcagctg tgccgcatga atatccaggt ggcagcaggc tcgattttct cggcttccgg
    34141 caaataccgt aattgtctac gcatcaactg cgctttgccg ctcagcgaaa cctatcgcga
    34201 agcactaaag caaattggcg aggccgtgta tcgggcaatg gaataaattc gaattagtgg
    34261 cgaagcacct gatttcctct cacgaatcgc ctgtaactcg ttgtatcttt gcagggtcat
    34321 cgccacgttc cagcatggct taggcaaagt gtgcttcgtt cataccggat gcggcgtgaa
    34381 cgccttatcc ggcctacaaa ccgcgcaaat tcaatagatt gcagaggaaa tgtaggtctg
    34441 ataagcgtag cgcatcaggc agctttgcgt ttgtcatccg tctctgatta gtattcgatc
    34501 catggatcgc gcttgatttc aggcaagggc tgctctaatg cctgcatttg ctctgtcgaa
    34561 agtccggtaa tcttttgtat ccattcagga tctgccccat tctgcaacaa caatctaagg
    34621 aggcgttgtt ccccttcttc tttccccttt tccatcccca acagccatcc atcattataa
    34681 attcgctctg caatcgtcat tattcgctcc ctgtgttgtg gcattcgacg ggtaagttcg
    34741 ctgataaacg ccttaaaacg cgcttcatcg ccagtcagta aaatgtaatt taacagcgca
    34801 gttatctggc tgtcattagc acactctgta accagtaata ctaccagttg gtcaataagc
    34861 cccatcagat cgcgctggcg aatatgcttt tggatcaact ccaacagggc gactctgcga
    34921 tgctgcacaa tctcgtcgtc tggcacgaca gtgacatcca ccagcgggaa cgctgcgcta
    34981 taaagtttcc gtgcggtagt cggggcggca aattcgtcca gccagcacag ggaccagggg
    35041 taaggactac ggctaccgtg ataaaacagc atcgggataa ccagcggtag cggccggcgt
    35101 ttatcatgct cgatatggcg ctgcatcacc gccatggaat atcgcatcag gcgaaacgcc
    35161 atatgaatat cctcgcggct ctgatgttca atcactacgt aaatataacc atcaccttca
    35221 cgggtcttta ccgaccacag aatatcggag tgtagcgccc gcaatttttc atcgacaaag
    35281 ctggcggatt ccagttttaa gctgtcgaga tcgcacagtt cacgtaaatc tttaggtaag
    35341 tggatctcca taaaatcccg cgcggtgcca gggtgcgtga gaaaggactt aaataatgcg
    35401 tcatgcggcg tgctggtcga gaagtttgtc atggcgatcc gtcacctgta gataagatgg
    35461 cccgacttta gcggctaaca aaaccattct cacccggcaa tttcccgttc cccgaggcgc
    35521 tttccaggct atttttattc ttctgcaaac gatacatttt ttcggaaaca ggctcgcgtt
    35581 ttacatttcc tgtacccggc agatattccc ctttccaccg cgctcttttg attaaccgca
    35641 aattttaatt acacttaagg tgtatatttt ctatgcaacc catcaattaa agaggtgtaa
    35701 tgtgctgatg actatttgta atcgttatac attctgacct ggagtcagaa ggtatttctc
    35761 tgtctgtgtg ttcacaggca gtgtggttga ttacatgaaa ttcagtacat ttgtccttct
    35821 cgcctgcctt tcgtcattac cgacggtatt gaatttcgtt ttccccgttg gggttctccg
    35881 gacaaggagt tgtttgttat gccacgtttt tttgcccgcc atgccgccac gctgtttttc
    35941 ccgatggcgt tgattttgta tgactttgcc gcgtacctgt cgacggatct gatccagccg
    36001 gggatcatta atgtggtccg tgattttaat gccgatgtca gtctggcccc ggcagccgtc
    36061 agtctctatc tcgcgggcgg tatggcgtta cagtggctgc tggggccgct ttccgacagg
    36121 attggccgca ggccggtgct gattaccggg gcgctaatat ttacccttgc ctgcgctgcg
    36181 acaatgttca caacgtctat gacacagttt cttatcgcgc gtgcaattca gggcaccagt
    36241 atctgtttta ttgccaccgt tggttatgtc acggtgcagg aggcgttcgg ccagacaaaa
    36301 gggatcaagt tgatggcgat tatcacctcc atcgtactga ttgcgccgat tattggcccg
    36361 ctttccggcg cagctctgat gcactttgtg cactggaaag tcctttttgc catcattgca
    36421 gttatgggtt ttatctcatt tgttggctta ctgctggcga tgccagagac ggtgaagcgc
    36481 ggcgcggttc catttagcgc caaaagcgtc tttcgcgatt ttcgtaatgt cttttgcaat
    36541 cggctgttcc tctttggcgc agcaaccatc tctttaagct atatcccaat gatgagttgg
    36601 gtggctgtct cgccggtgat cctgatcgac gcgggcggct taacaacttc gcagttcgcc
    36661 tggacacagg ttccggtgtt cggcgcggtg attgtcgcga atgccatcgt ggcgcgtttc
    36721 gttaaagatc cgaccgaatc gcgctttatc tggcgtgctg tacccattca actggtcggc
    36781 ctcgcactgt tgattatcgg caatctgctg tcaccgcacg tctggctgtg gtcggtgctg
    36841 ggcaccagtt tgtatgcttt cgggattggt ttgatcttcc cgaccttatt ccgctttacg
    36901 cttttttcca ataacttacc gaaagggacc gtctccgcat cgctgaatat ggtgatcctg
    36961 atggtgatgt ctgtctcggt cgaaatcggc cgctggctgt ggtttaacgg cggtcgcttg
    37021 ccgtttcatc tgttagccgt cgtggcgggc gttatcgtcg ttttcaccct ggcaggattg
    37081 cttaatcgcg tgcgccaaca tcaggctgcc gagctggcgg aggagcggtg atttttgcgc
    37141 gatcctgccg tcaggctcta ttcttaacgt tatgaataaa ctcattgaac tcagacgcgc
    37201 caaaatgttg gcgctctctt tactgcttat cgccgctgct acctttgtcg ttacgctgtt
    37261 tttgccgccc aatttttggg tgagcggcgt gaaggcgatt gctgaagcgg cgatggtcgg
    37321 cgcgctggcg gactggtttg cggtggtggc gctgtttcgc cgcgtgccga ttccgatcat
    37381 ttctcgtcat acggcgatta tcccgcgtaa taaagaccgg attggcgaaa atctcggcca
    37441 gttcgtgcag gaaaaatttc tcgataccca gtcgctggtg gcattgattc gacgtcacga
    37501 accggcgttg ctgattggca actggtttag ccagccagaa aacgcccgcc gcgttggtca
    37561 gcatctgttg cagatcatga gtggttttct tgaactgacc gatgatgcgc gtattcagcg
    37621 cctgcttaag cgcgcggtcc atcgggcgat tgataaggtc gatctttccg gaaccagtgc
    37681 gttgatgctg gagagtatga ccaaaaacga tcgtcatcag gtgctgctcg atacgctgat
    37741 cgcacagttg atcgcccttc tccagcgcga taaatcgcgc aagtttattg cccagcagat
    37801 tgttcgctgg ctggagagtg agcatccact gaaagccaaa attctcccca ccgaatggtt
    37861 gggcgaacat agcgcggagt tggtttctga cgcggtgaat tctttacttg atgatatcag
    37921 ccgtgatcgt gcgcatcaga tccgtcatgc gtttgatcgc gccacttttg ccctgatcga
    37981 caagttgaaa aacgatccgg aaatggcagc gcgagccgat gccgtaaaaa gttatctgaa
    38041 agaagatgaa gcttttaacc gctatctcag tgaattgtgg ggggatttac gggagtggct
    38101 gaaagcggat atcaacagtg aagattctcg tgtgaaagaa cgtatcgcgc gggcgggtca
    38161 atggtttggc gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa atggtcacct
    38221 ggaacaagcc gcgcaccgcg tcgcgcctga gttttccgca ttcctgacgc gccacatcag
    38281 cgatacagta aaaagctggg atgcgcggga tatgtcgcgg caaatcgagt taaatatcgg
    38341 caaagatctg cagtttatcc gtgtcaacgg tacgctggtt ggcggttgta ttgggctaat
    38401 tttatatttg ttgtcgcagc tcccggcctt gttccccctc ggcaattttt agaaatccat
    38461 gaaaaactaa gtggctaacc gcgagagaga tcaaataaac cacactaaaa gaatggtaat
    38521 aatgtggcct cttttcatta tctcccgtgg tacggggaag gaaaatcatg tcacttatca
    38581 ccgatctacc cgccattttc gatcagttct ctgaagctcg ccagaaaggc tttctcaccg
    38641 tcatggatct caaggagcgc ggcattccgc tggttggcac ttactgcacc tttatgccgc
    38701 aagagatccc gatggcagcc ggtgcggttg tggtttcgct ctgttctacc tctgatgaaa
    38761 ccattgaaga agcggagaaa gatctgccgc gcaacctctg cccgctgatt aaaagcagct
    38821 acggcttcgg caaaaccgat aaatgcccct acttctactt ttcggatctg gtggtcggtg
    38881 aaaccacctg cgacggcaaa aagaaaatgt atgaatacat ggcggagttt aagcccgttc
    38941 atgtgatgca gttgccgaac agcgttaagg acgatgcctc gcgtgcgtta tggaaagccg
    39001 agatgctgcg cttgcaaaaa acggtagaag aacgttttgg gcacgagatt agcgaagatg
    39061 ctctgcgcga tgccattgcg ctgaaaaacc gcgaacgtcg cgcactggct aatttttatc
    39121 atcttgggca gttaaatcct ccggcgctta gcggcagcga cattctgaaa gtggtctacg
    39181 gcgcaacctt ccggttcgat aaagaggcgt tgatcaatga actggacgcg atgacagccc
    39241 gcgttcgtca gcagtgggaa gaaggccagc ggctggccct gcgtccacgc attttaatca
    39301 ccggctgccc gattggcggc gcagcagaaa aagtggtgcg cgcgattgaa gagaatggcg
    39361 gctgggttgt cggttatgaa aactgcaccg gggcgaaagc gaccgagcaa tgcgtggcag
    39421 aaacgggcga tgtctacgac gcactggcgg ataaatatct ggcgattggc tgctcctgtg
    39481 tttcgccgaa cgatcagcgc ctgcaaatgc tcagccagat ggtggaagaa tatcaggtcg
    39541 atggcgtagt tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa tcgctggcga
    39601 ttaaacgtca tgtgcgtcag cagcacaaca ttccttatat cgctattgaa acagactact
    39661 ccacctcaga tgtcgggcag ctcagtaccc gtgtcgcggc ctttattgag atgctgtaag
    39721 gagtggcagt gacatattcg attggcattg attctggttc taccgccacc aaagggatct
    39781 tactggcaga cggcgtgatt acgcgccgtt tcctcgttcc aacacccttt cgtccggcaa
    39841 cagcaattac tgaagcctgg gaaactctgc gcgaagggtt agagacaacg ccgtttctga
    39901 cgctcaccgg ctatggacgg caactggtgg attttgccga taaacaagtg acggaaatct
    39961 cctgtcatgg gctgggcgca cggtttcttg cgccagcaac gcgcgcggta atcgacatcg
    40021 gtggtcagga cagcaaagtg attcagcttg atgatgacgg taacctgtgc gatttcctga
    40081 tgaatgacaa atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg
    40141 gcaccagcgt cgagcaactc gacagcatta ccgaaaatgt cacgccgcac gccatcacga
    40201 gtatgtgcac agtgtttgcc gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc
    40261 cagaagcgat tctcgccgga gtgattaacg cgatggcgcg gaggagtgcc aatttcattg
    40321 ctcgtctctc ctgtgaagcg ccgattctgt ttactggtgg cgttagtcat tgccagacgt
    40381 ttgcccggat gctggaatct catctgggaa tgccggtaaa tacccatcct gatgcgcaat
    40441 ttgctggcgc aattggcgcg gcggtaattg gtcaacgagt gaggacacgc cgatgaaaga
    40501 gtttttattt ttatttcact ccacggtcgg cgtcatacaa acccgcaagg cgttgcaggc
    40561 agcgggcatg acttttcgcg tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt
    40621 atgcatttgg ctgacctgtc cccccggcga ggaaatacaa tgggtgatcc ctgggcagac
    40681 tgagtcaatt tattgccagc aggatggtgt ctggcgctgc attgcacatt acggggtttc
    40741 tccgcgttaa ggattcatta aggtcgctac gtatacttct cgatatctta ttgatttgcc
    40801 ttaaagggat taccgtgaca aaaagtattt cgttaagtaa acgcatctcc gttattgtga
    40861 ttctttttgc cattgtggca gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca
    40921 atcatgcagc atcatttcag aattaccacg ccaccattga cggcaaagaa attgcaggca
    40981 taacgaataa tatctcttcg ctgacctggt cggcacaaag caatactctt tttagcacta
    41041 tcaacaaacc tgccgccatt gttgaaatga ccaccaatgg tgattttatc cgcacaattc
    41101 ctctggattt tgttaaggat ctcgagacga tcgaatacat tggcgataat cagtttgtca
    41161 ttagcgatga gcgcgattac gccatttatg tgatttcact gaccccaaac tcggaagtaa
    41221 aaatcctcaa aaaaataaaa atcccgctgc aagactcccc aaccaattgc ggttttgaag
    41281 ggttggctta ttctcgccag gatcacactt tctggttttt taaagagaaa aatccgattg
    41341 aggtctacaa agttaacggg ctgttaagta gcaatgaatt acacatcagc aaagacgagg
    41401 cactacaacg gcaatttact ttagatgatg tgtccggcgc agaatttaac cagcaaaaaa
    41461 atacgcttct ggtgctatct catgaatctc gcgcactcca ggaagtaacc ctggttggcg
    41521 aggttatcgg tgaaatatca ctaaccaaag gtagtcgagg tttgtcgcat aacatcaaac
    41581 aggccgaagg gattgcgatg gatgcctctg gtaatattta tatcgttagc gaaccaaatc
    41641 gtttttatcg ctttacccct aaatcatcgc attaacaggc tggcatattt cacctttttg
    41701 ttattaactt gttaacaaca aaaatgacat acaaggttaa acatgccttc gtcctcgcat
    41761 cccgtagaac gcttttcttt cagcaccgcg cttttcggga tgctggttct gaccttaggt
    41821 atgggattag gtcgcttcct ttatacgcct atgttgcccg tcatgatggc ggaaggggcg
    41881 ttttcattta gccagctctc gtggattgcc agcggcaact atgcggggta tctggctggc
    41941 agtctgctat tttcgtttgg cgcatttcac cagccgtcgc gcctgcgccc attactgttg
    42001 gtttctgccc tggcgagcgg gttgttgatc ctcgcaatgg catggttgcc gccatttatt
    42061 ctggtgttat tgattcgctt cctggcgggt gtcgccagcg ccggtatgct gatttttggt
    42121 tcgacgctga ttatgcagca cacgcgccat ccttttgtgc tggcagcttt gttttctggc
    42181 gttggcattg gcattggcat tgcactgggc aacgaatatg ttctggcagg cctgcatttt
    42241 gccttctcct cgcaaacgtt atggcaaggc gccggggcgc tttctggcat gatgctgatt
    42301 gcacttacgc ttttaatgcc ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa
    42361 acggagcaac agataatgag ctggtggtta ctggctattc tgtacggcct tgcgggattt
    42421 ggttatatca tcgtcgcgac ttacctgccg ctcatggcga aagatgcagg ctcaccattg
    42481 ttaaccgccc atctctggac gttagtcggc ttatcaattg tgccaggctg ctttggctgg
    42541 ctatgggcag caaaacgttg gggggctttg ccttgcctga cggcgaattt gctggtgcag
    42601 gctatcagtg tgctgcttac tctcgccagc gactctcctc tcttgcttat cattagtagt
    42661 atcggttttg gcggtacctt tatgggcacg acatctctgg tgatgactat cgcccgccaa
    42721 ctgagcgttc cgggaaatct caatctttta ggctttgtga cgctcattta cggcattgga
    42781 caaatcctcg gcccggcgct gaccagtatg ctcggcaatg gaacatcggc gcttgccggc
    42841 gccactctct gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac agtacaatta
    42901 tttaagctgc aagtggtcac ttcatgagga gtgtttccct taccactcaa gaaactcaac
    42961 agcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga tgcctcgttt
    43021 cgccatttcc tgtgctttga cggttaagat aaccggcgat ccgtgtcttg ctcccacttt
    43081 gcgtgccgtt gcttcatcag cagagagatg tacgtaatga cgctcccctg caatcagccc
    43141 ctgttttttt atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa actgtggcgg
    43201 tgttttttcg gcaaaggaaa tggcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat
    43261 gcacctacca tcactggaat aactaaaacg ctttttatcg ctggtggcaa ccacagtatc
    43321 cagcaatgca cgggttaacc gcttgcccgc tttttgggca caaagtatga gcttatcgat
    43381 atccgcccat ccttcacggt caagtacgat accaatcgcc tccggtttgt gtcggaggac
    43441 aaaacttaaa aacttgctcg tatccgccaa ctctttttcg ttgtactttg ccatcgcgtt
    43501 ttcgtccctg gattttctgt ttttatttac tgatgtcatt gtcactgggt ttatgaattt
    43561 tctgcaactc aatcctgcgc agagcctttt ctacacagcg taatttttcc gctatgcaaa
    43621 aaacgcatag caagaaacat acttgcattt tccccatacc cacatggctg ataaggtttt
    43681 tcagctccag gaattttcct ggctcaagca acacacaaac acaaaaaaac aatggggatt
    43741 gttatgacac aacaagggga tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt
    43801 aaagggtatc tggctttttt tctcaccata atcttttttt ccggagtttt ttccgggaca
    43861 gacagctggt ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg gcaactgcct
    43921 ggtgcgaatg gagccaccac ctcatttcgc ggggcgggtg gcgcgggggc taaagatggc
    43981 tttctctttg cgctggagct ggcaccgtca gtcattctct ctctggggat tatttcaatt
    44041 accgatggtc ttggtggatt acgcgccgcg cagcagttaa tgacgcctgt gctaaaaccg
    44101 ttgttgggca ttccagggat ctgctcgctg gcgctgatcg ctaacctgca aaacactgat
    44161 gccgccgccg gtatgaccaa agagctggcg caggaagggg aaattaccga acgcgataaa
    44221 gtgattttcg ccgcatatca aaccagcggt agcgccatca tcaccaacta cttctcttcc
    44281 ggcgtcgcgg tgttcgcctt tctgggcacg tccgtaatcg tacctttagc cgtcatcctg
    44341 gtgtttaaat ttgtcggagc caacattttg cgcgtctggc ttaacttcga agaacgtcgc
    44401 aacccaacgc aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc atggatatgt
    44461 ttatcgatgg cgcgcgccgt ggctttacta tcgccacgac caacctgctg ccaaacgtgg
    44521 tgatggcgtt tgtcatcatt caggcgctga aaattaccgg cctgctcgac tgggtggggc
    44581 atatctgtga acccgtaatg gcgctgtggg gattacccgg tgaagcggca accgtccttt
    44641 tagccgcatt aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg gcgaccgccg
    44701 gcgcattaac cggtcacgat gtcaccgttt tactgcccgc gatgtattta ataggcaacc
    44761 cggtgcaaaa tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa tattacccgc
    44821 atatcattac ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg cagcttattg
    44881 tttaacaagg agttaccatg attgattata ccgcagccgg ttttaccctg ctgcagggag
    44941 cgcatttgta tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa
    45001 ttatcgccgt tgccagcaat atcccttctg acattgtacc ggactgcacg gttgtcgatc
    45061 tcagcgggca gatcctctgt cctggtttta ttgatcaaca cgtccatttg attggtggtg
    45121 gcggcgaagc aggtcccacg acgcgcacgc cggaagtggc gctaagtcgc ctgacggaag
    45181 cgggcgtcac gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc cacccggaat
    45241 ccctgctcgc caagacccgt gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg
    45301 gcgcttatca tgtcccttcc cgcaccatta cgggttccgt ggaaaaagac gtggcgatta
    45361 tcgatcgtgt gattggcgtg aaatgcgcca tctctgatca ccgttctgcc gcaccggacg
    45421 tttatcacct ggccaatatg gcggcagaat cccgcgttgg cggtttgctc ggcggtaaac
    45481 ctggcgtcac cgtgttccac atgggcgaca gtaaaaaggc gttacagcct atttatgacc
    45541 tgctggaaaa ctgcgatgtg ccgatcagca agctgctgcc gacccacgtt aaccgcaacg
    45601 taccgttgtt tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc gatatcacca
    45661 gcagcattga cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt caggcgggta
    45721 ttccgctggc acgcgtcacc ctcagctccg acggcaacgg tagccagccg ttcttcgatg
    45781 acgaagggaa tttaacccat atcggtgttg caggttttga aacgttgctg gaaaccgtgc
    45841 aggtgctggt caaagactat gatttcagta tcagcgatgc cctgcgcccg ctcaccagta
    45901 gcgtagccgg tttccttaac ctgaccggga aaggtgaaat tctgccaggc aatgatgctg
    45961 acttgctggt catgacgcca gaactgcgca ttgagcaggt atacgctcgc ggcaaactga
    46021 tggtcaaaga cggcaaagcc tgcgtgaaag gaacgtttga aacggcttaa acggttgtca
    46081 gccgcccgaa tggacgtaga atgcccgcaa gggcggctga cagagtaaaa cgtaatggat
    46141 gactgtggtg cgattttgca taatattgaa accaaatggc tttatgattt tctgaccctg
    46201 gaaaaatgcc gcaatttttc ccaggcggca gtcagtcgca acgtctcgca accggcattc
    46261 agccgccgca tccgtgcgct ggaacaggcg attggtgttg aattgtttaa ccgccaggta
    46321 acgccgctgc aactctcaga acaagggaaa atcttccatt cgcagatccg ccatctgttg
    46381 caacagttag agagcaatct ggcagagctg cgtggcggca gcgattacgc gcaacgtaaa
    46441 atcaagatag ccgctgcaca ctctctttcc ctcggtctgt taccgtccat tatcagccag
    46501 atgccgccgc tctttacctg ggcaattgaa gccattgatg tcgatgaagc ggtcgataaa
    46561 ctgcgtgaag ggcaaagtga ctgtattttt tcctttcacg acgaagattt gctggaagcg
    46621 ccgtttgacc acattcgctt atttgaatct caattgttcc ctgtctgcgc cagtgacgaa
    46681 cacggagaag cactttttaa cctcgcgcag ccacactttc cgttactgaa ttacagccgc
    46741 aactcctaca tggggcgatt gattaatcgc accctgacgc gccacagtga gttaagtttc
    46801 agcacctttt ttgtctcttc gatgagcgag cttttaaagc aggttgccct cgacggctgt
    46861 gggattgcct ggctgccgga gtacgccatc caacaagaaa ttcgcagcgg gaagctcgtt
    46921 gtactgaatc gggacgaact ggtgatcccg attcaggcct atgcataccg gatgaatacc
    46981 cgcatgaatc ccgttgccga acgcttctgg cgtgaactgc gcgagctgga gattgtgctt
    47041 agctgacatt ctccagcgtc gcactgcgcc aaccgccatt atcccagacc agatcaaacg
    47101 ttagcggttc atcgtcgtgg tacagcatta tctcaaggct gatcgccaga gtgtggcagt
    47161 acgcttcttt cagattaacc tgcaacgccg caatccgtgg ctcatgaaat aacagtgcag
    47221 aacgcagctg acggcaatac cagctctgga gatgtttgtc gctcgggtta tgcacttgcc
    47281 actcgggcat gcctgaaaca agtaatgacg gtaatgccga atgaggagaa ttaaaaagag
    47341 tgaggagatc gctgcatacc gatttacgca gcaatgacgt tttattgcct gaaatttcag
    47401 gtaaaacagc ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt tagcaacata
    47461 tgcggcttgc cctgaacacc gggcaccact gtattgatgc tcattttcca tagccattcc
    47521 cttttgttca ttacatccat gtcagtctga gacaggcctg aatcctgtca gcaaagtata
    47581 aaaccttact tgcctatgaa tatctactga cttttcagga aaaaagtcat gatttgataa
    47641 aaatcatcaa actcaaaaaa aaaccgctag tatattctga atattccgaa tagttatgta
    47701 gatgaaaata cttgtcaccg ccgctactta attccggtgg gaaattattt atttccgcat
    47761 aatcagccat gtattatttg aaacaattag tttgtaaata atataaattg ataacatatc
    47821 aaatacatca aaaataccat tcacaggcat tattaatata gacatcaata tagcaacatc
    47881 aaattccaat tagtaataag atatgctttt taattgcatg caaataattt taaatgagcg
    47941 ttttttgata ctttagagtg tttcctaccc tttaatatca ccatgtagag ttgatatcac
    48001 tacaacagaa acatgcatgt catttgttta cttctttttt actacagaaa aatttatcta
    48061 tttttctaaa catcagtgaa atactcataa aaggtaacta ttatgtctat gccattaagc
    48121 aatgcccttc agagtcaaat tattacagat aatcacttcc ttcatcatcc aaaagtcgac
    48181 agtgagttaa cacgtaaata tgaacgtgca cgcctggata aggaaaatat ctatcttctg
    48241 cctcttgctc gtggcaataa ccataattac gatggaaaaa gcgttgtcga aattagaaaa
    48301 cttgatatta gtaaagagcc ctggccattt aattatgtga cagaaacatg cagagaatcc
    48361 gatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta
    48421 gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ccacagagtt agcagaagga
    48481 ttacggctaa gtttatttat gaagagccct tcctatcctg ttgaggacta tacagttcat
    48541 gaaataacat taggccctgg ttgcaatgtt ccaggttacg ccggaaccac gataggatat
    48601 atttcaacac ttccagtttc tcaggcaaaa agatggacca atgagcaacc aaggattgat
    48661 atctatattg atcagataat gactgtctca ggggtggcaa actcttccgg attcgccctt
    48721 gccgcattac ttaatgctaa tattgagttg ggtaatgacc caataattgg tatagaagcc
    48781 tatcccggta ccgcggagat ccatgcaaaa atgggatata aagttatccc gggtgatgaa
    48841 aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc ttccagaact gtttgaatta
    48901 aaaaatggtg aatggaatta tattggtaaa tagccactga gaagaatgag tactaacatt
    48961 caggttatac agatagctaa acaataccct gcaatttaca aagtaatact cattgcgact
    49021 tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat aagtcaggcg
    49081 tcgactttat ccagcggcca tgaatcaaac agatagccat caaaataaat atcgtcaacg
    49141 ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc
    49201 gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc gcccaaccac
    49261 tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat tggattgttt
    49321 tcccgccact gccaggactg acggaacagc tccaccagca tgctgttatg cgttgcttcg
    49381 gcaatagcga gatggaactg catgtcaccg ctttcgctgc tgcccggcgc actggaagcc
    49441 agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc
    49501 tgcaaagcgg caaactcggc gatgttgctc tccaataact gccgcgcctg taacagctca
    49561 aaaggaccgg catcgttgca gacgttggca tccggactgt ctgtgttctg actgcctgag
    49621 ttatcaagaa catagatacc ggcaccccgg cgtacttcca ccagcccttt gatctccagc
    49681 atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc
    49741 tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc gcggatcatc
    49801 gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt gttgtgtccg
    49861 ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa
    49921 cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac
    49981 gtataggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa
    50041 gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca
    50101 cagctgctga tatgccgccg tcactgcgcc aacaaagtcg gcattctgcg gcagatcatc
    50161 ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc
    50221 gttgaccttc tggaactccg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc
    50281 atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt gacgccagct
    50341 gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta acttctggct
    50401 gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat
    50461 cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg ttggcgcttg
    50521 ttcctgcatc atcaaggaga aggccgcttt gcgataatcc gggttagtca tggtgtcggc
    50581 aatggtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc tgccgttcag
    50641 catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg cgcccacttt
    50701 atcccagtcc gggcgaccgt taacgaagtt atcttcaatc acccactgac ggaacggttc
    50761 gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct gcaaggtttc
    50821 tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga cgttttcttc
    50881 aatccatgcc gccagctgcg ggtcacgcgc ctgagccagt cccagtaccg cgaccttcgc
    50941 cacatggccg ttttcacgca cgttatcgca ggacatcacc gtaaacgctt tcaacccttt
    51001 ttcacgacgc agccgcaggg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg
    51061 gttttccaga tcgtgcttga tcagtgggtt attgagatcc agctgaccgc ttgccgcatc
    51121 agcgcagtag cctttttccg tgactgtcag agagacaatc gccgtctgcg gacgcgccat
    51181 cgcattgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt tcatcgaacc
    51241 gataattttc agctcggtgc tctctgcgcc tttttccgct acggtgtaca gcagttgctg
    51301 ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa cttcgcagat
    51361 gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct ggtgcgcgcg
    51421 gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga
    51481 cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg
    51541 tcttcaaaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca tgccggtgga
    51601 tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg
    51661 cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag gtcgtccagc
    51721 atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga
    51781 cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg
    51841 tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg
    51901 aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc agcacgcacg
    51961 ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc
    52021 cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga
    52081 tcgtccgggt gaacagccat acggacgcca acttcttcgg caaccggaat aatcgctttc
    52141 aggaagacag caaagttttc gcgcagtttg gctttgtcga tatctttgta cagctccagg
    52201 tgcttacgga actggtcgag ggtgtaccct tcttccgcac ctggcagacc agcgatgatg
    52261 ttacgggtca gacgcgcttt gtcttcatcg ctcatggtgg cgaagcgaac agcagcctga
    52321 gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg
    52381 aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg cagcacgtat
    52441 tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg
    52501 ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg
    52561 ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg
    52621 cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg
    52681 ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct
    52741 aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt
    52801 tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta ggtatccgga
    52861 tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct
    52921 tttggtaaga catgtcaacc cgaattctga aattggttaa ccacatcaca agaatttcac
    52981 tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg atcagaggat
    53041 gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca aaataacaac
    53101 ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctttgg gtaatattcg
    53161 gcattggcct gatgctggta ctgaatttga agttcaaaat caattcaatg gtggctttgt
    53221 tggtggcggc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg ctgctgcaca
    53281 ccatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg gtgttcggtg
    53341 cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagca catacgctgc
    53401 tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc ctgattttcg
    53461 gtctggcgat gttttatgaa gtggccttta tcatgttagc gccgctggtt attgttattg
    53521 ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca gctgccacta
    53581 ccgcacattc attgttccca ccgcagccgg gtccggtggc gctagtgaac gcttatggcg
    53641 cggatatggg gatggtttat atctatggcg tactggtgac gatcccaagt gtaatctgcg
    53701 caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca tcattcctga
    53761 aagcagatca accggtagat atgaataatc tgccctcttt cggcgtttcg attctggtgc
    53821 cgctgatccc agcgatcatt atgatctcca ccaccatcac caatatctgg ctggtaaaag
    53881 atacccctgc gtgggaagtg gttaatttta ttggttcctc gccgattgcg atgtttattg
    53941 cgatggtggt tgcattcgta ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga
    54001 tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc atcggtgcgg
    54061 gtggcgtgtt gaagcagacc atcatcgaca ccggcattgg cgacaccatc ggcatgttga
    54121 tgtcccacgg caatatctcg ccctacatca tggcatggct gatcaccgta ctgattcgtc
    54181 tggcgacggg tcagggtgtg gtttcagcga tgaccgccgc cgggattatc agtgctgcaa
    54241 tccttgatcc agcaactggt cagctggttg gcgtgaatcc ggcgctgctg gtactggcga
    54301 cggctgcggg ttccaacacc ctcacccaca ttaatgatgc ctcattctgg ctgttcaaag
    54361 gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg gagctggtca
    54421 actccgtggt tgggctgatt attgtgttga ttattagcat ggtagcgtaa aaggacacca
    54481 gagccagcca atggcaggct cagactgatg acaaagtcta aaaatgtgcc cggacagtcc
    54541 cctcgcccct ccggggagag ggttagggtg aggggaacag accagcaaag tgcgctttgt
    54601 ttatgccaga tgcgacgctg acgcgtctta tctggcctac aaagggctaa cgtgcaggtt
    54661 tttagcttca ggtaatattg cgtaccagca ttagcaatgt cctgtgattt ctttattgat
    54721 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccggtac
    54781 gtgcataatt tgccgttaat cccagactca ccgccgaagt ccctactgct cctaaagata
    54841 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgtg
    54901 caggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg gttgtgccgg
    54961 agaggtaata ccccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
    55021 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
    55081 taggcaccac cacatcatta ttggcgtaaa tattccacac aaactggaaa tcatcgctgt
    55141 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgagccagct ttaatcgcca
    55201 ccccgcccgc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
    55261 tcgaattata aacaacgcgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
    55321 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
    55381 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
    55441 gatccacgac caggttttgc cccacattca cggcgggcgc aaggtttaca taaacattgg
    55501 cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg aatgaccagg
    55561 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
    55621 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
    55681 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
    55741 gaggaatcat ccacctgaac tgttttggtt gcgccagtat tcaatgtgtt gccactgtca
    55801 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtgctg
    55861 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
    55921 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
    55981 agatcgccga gatcaaccgt ggcattggtg gtggaaactg tgcacggttt ggcgacgacc
    56041 ttaccgttga ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaat
    56101 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
    56161 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
    56221 attagccggg cgtaaaaatt caacgtattt ggtttacccg gcgtcagggt cgtccacgaa
    56281 atcgcggacg atggagcatt aaggggtatt tgattttgct gctcattcag aagctgtatt
    56341 cccagtcccg aagccgctga caccgtattt tcaagtgcaa gcagattggc attgtggcta
    56401 tctgcaacgc cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
    56461 aaaatacgaa atggaacaac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
    56521 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
    56581 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgtgact caccgccagc
    56641 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
    56701 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
    56761 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
    56821 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
    56881 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
    56941 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
    57001 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgccactctg
    57061 ttttccctat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
    57121 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
    57181 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccaatacccc accgctgact
    57241 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatattggc attgccgtaa
    57301 ccaccgcgat aattcagcgt ggcgtagcct gtgcttccgc tattaccatc gcctcccccg
    57361 gcatagccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
    57421 ccagccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtagct ggcactggca
    57481 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
    57541 agcgctaaca tctgatcacg tcctttttgc caggcgtttt tcgtcaggct atagctgagc
    57601 gtccagttga tatcttcgaa tgcagtattt aatccagcct ggaattgctc atcgacatta
    57661 ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatgt gcgcccgagt
    57721 tgctgagtaa cggtgagctg taatttcccg cgtttgttat aagcgaggtt gtaatagtcg
    57781 gtgaatttcg gcttaacctg aataactccg tcctgtgttt cgatgttgta gccattcatt
    57841 cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg gtaacccact
    57901 aactgaatat tcgtgcctga ctcattgagc gatttgttat agagaaaacg caccgattgt
    57961 ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc cacagacaga
    58021 gcgcccagtg cccccatgtt tttcccgata ccgaaattaa aagcacgata acgatccgcc
    58081 agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa tgtactctgg
    58141 aaaaagcggg gtttttcctg ctgcgcattt ccactacggt attctcctgc cgtaatggaa
    58201 taacgagtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac ggtaaaaatc
    58261 tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt caccactatt acctgcggca
    58321 tagatatcgt tgatggtaaa aggccccggt ggcaccgtac tattataaat gtcataccca
    58381 ttttgtttaa tagtgacctg tgcagtacca cgagcaatac cgtggatcac cggggcaaat
    58441 cctctttggc tatcgggtaa catattgtca tctgaggcca attgtgcgcc gcgaaagtta
    58501 ataccatcga aaatatcgcc ctgagtataa ccatcaccca gcgtcagccg ggaacgtaac
    58561 ggtattatgt ctcgctcaag ccaggtattg atatgctgcc atttattttt gctacctgat
    58621 gatctgtcgc tactgttata actccaggtg gtattgtcgc gtaaacgcca cgcaccaata
    58681 tttaacccac tctgtaggtt taaatatgca taatggctgt tacccccaat ccgattctgt
    58741 acactatttc cgctgaaatt ataattgagc aatccggcat taataccggg atcccataac
    58801 tcaggaggaa tataaccacg cgcgcgatta ctcataaatg cctgagggat cgtcaggttc
    58861 agtcgctgct gaccaacatc cagatgcgca gtagcgtcct ggatcattgt ggttaatggc
    58921 acgcaggcat catccgccag cagattcata ccggagacag aagccgtatt cagccccata
    58981 ctggcaagtt gcgcgcgtgt caggcaggga acaatccctt gttcactgtc gcccgtatta
    59041 aatgtgacat cacgcgttgc catataacca ttattcaaat agatatcgac acgatacgtc
    59101 cctggcggta attcttgccc attttcaaaa cgcgataaat cggccacagc ctggggatca
    59161 tccgctaaaa aacgcgggtt aaaatagagt tcggcagatg acaaaggtgc ctgtgcggca
    59221 aaagcacagg caacaaagag ccggacaaaa aaaccagcca aacgatgctt acgaatatgc
    59281 aagcattgtg tgtttcgctg gtaaagtctt aaattcagat atgacattat tcctccggtt
    59341 taaagtcacc cggcagtcaa ttctttttat tcaggcgaaa aattccccct gcgttattcc
    59401 attacgcccg tcattttggg ggtaagtgca ccataatcat ttattgttcg gtaagtaata
    59461 ttgcttcccg catcagaagg caatttaacc gtgctttcgc ccattggagg caccaaggca
    59521 ttttcaagaa cccgggttcc ggcattcaac tctgttaccg tcaggtaata gggtgtcggg
    59581 ttaatcagcg tcagagaatt cgcgctacga cgaaatctta atttttctgc ggcctgatcg
    59641 ggtggcaacg ctaatttagc cgggcgatag tacagtttaa tgcggctgat aattgcgagc
    59701 tgtagcgtat tctcagtcaa ttttgattta tccattgacg gaatcgcttt aacattcatc
    59761 cagaataaac tttcccgatc ctgtggcaat tggttatttg ttgcatcaag aatacgcaag
    59821 gtattctctt tttttccctt catcgcaaac agaggaggcg tcacgataaa acgaccatcc
    59881 tttacaccat cggcattttc cacccatgat tgaattaaat aggtactatt ttcatcatta
    59941 tttgtcacgg caagttgcac ttgtttttgc cctgccggat aaattacgcg agtcgcacct
    60001 aaggccactc ccgcttcagc gcgtccggca accatcattg ccataaacat caggatacct
    60061 gccagcaagc agaatgttat ttcctgcgat ttccttacat tgacgttttt attactcact
    60121 gtcctgttcc tgttatcaca ttatctgctg aacaattact gataggttaa agagaaccag
    60181 gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg tagcacgata tttggcgatg
    60241 aaatgtagcg aagtagagcc tgagtaaagc cgtttccagt ttgctggagg acgattaatc
    60301 ggtacgaggt ttccttcatc atcaaacaac gctacgccaa tattggtggc tatccctggc
    60361 ccctctccca cggaaagcac atccggattt ttaccatccg cgacaccgtg aaacgccacc
    60421 cccacacgtt cactcaccac cgtgctacat tcccgtaaat gaataacaaa aggcaccggt
    60481 gcgctatctt cccctgccgc atgaaaccgg ttactgctga tttgccccat attgaccgtc
    60541 atttgtttat caccggcttc aatccggcaa gtttccgcaa taatgacgcc ctgaaattgc
    60601 atatttccgc caggcagcgt ggtattccat ttatttccgg ccagagcaaa cattggcagc
    60661 aacgacgcta acagaaataa tcttttcctt ttcatcgtta tttttatcgc acaagggtgg
    60721 gcatccctgc ccgtaatgac gtccttgaac ctgggtaggt tattgatact gaaccttgaa
    60781 ggttgcatcc gcattagctg cacccggggt tgcctcgccg attgcataat aacgcgcctg
    60841 gaacggaatg gtgttggtac cgttattcag ggttgtttgt gcactaaatg tcgcaccgtc
    60901 cagcgtcaga gcattgcctg ttctgtccag gatctgcacg ccaacgtttg ttgcactacc
    60961 tgcagcagaa ctctgcagag ccagtacatc agtacgcgta gcatcaattg ccgtacctaa
    61021 gaaggcaaca gcggcttttg tggcaacagt ggtatcgcaa tcattcagct gaatgttaaa
    61081 accaacggca gagctggttg ctccagcctg cttcaggcta gcggtacgaa cctgtcccaa
    61141 ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa gcggcgttaa caacttcccc
    61201 tttaaagtga acggtcccac catttaccgt cgtagtatcg gccagagccg ctgcggaact
    61261 gagggacaga gccgacagaa caacgattgc cagagtttta attttcatgc tgctttcctt
    61321 taaaaaaact atttctaaat cgacatgggc agtcgttctg tacactttgt tttgtcaacg
    61381 agtttgactg ccaacactgc acagttttcc cccaaaagat gaaacatttg gggccatttt
    61441 gactcataga ggaaagcatc gcgcacaaac tttttcagtt tatttgttgg cttaatattc
    61501 tattgttatc tttatttata gatttttata ttgcatgagg tggtttttgg agagaagaat
    61561 gaggaagatg cgtcgagcca cagaaacgtt agctttacat atagcggagg tgatgtgaaa
    61621 ttaatttaca agagaaataa tttacatatc aaacagttag atgctttttg tcgtttttta
    61681 atatttttat gcttgagaaa aaatacgtaa cttatttatg atatggacag tttggcccca
    61741 attgtcttgt atttatttgt tttttaactt tattatcaat gagttaaatc aaacctcttc
    61801 tctttttaat ttttcgttta tgagattatt tctttcccat agtccggcaa aacgagcagc
    61861 attactggcg gtataacgta cagtatggcg aatatttcga tgcccgagat aatcctgaat
    61921 taaacgggta tcggcaccac gctccgccag ttcataacca caagcatgcc ttaacatatg
    61981 aggatgagtc tgcgttacgg ttccagcttc aataccggca tcgcgaataa tgcgataggc
    62041 ctgctggcga gaaagccgac tcccgcggcg agaaataaat atagcgtcgg tccggtcagc
    62101 gcctttccag ttagcacgtt cctgggtcca gcgttccacg gcttcacgct catcaaaacg
    62161 taacgggtga acggtagaaa atccgttctt cagtcggcga atatttattc gaccttcatt
    62221 aaggtcaaga tcctgataat gcagatcaag cagttcacta atacgcatcc catgccgata
    62281 tgccaacaga ataagacaat aatctctggc tcccgttgcc ccgtaacaaa ccgcctgcat
    62341 catggcctga acttctttac cggtaagata acgacgttta ctcacaatag tagtactcct
    62401 aactgagata tatttgaatg aatacctata ggaaacctca atcggtcaaa attagcccaa
    62461 tagataaaaa gataaccaca cacggagtga tgtggctatc agtcaattac attaaatatc
    62521 atacaaaaat aaaatatcac tgatgtgaca acatctattt tcattgattt gattcaatat
    62581 aacaatagat tgcagtggat taacttccag ttgcttaaaa aataaagaaa aaagtgcgga
    62641 aacaggagaa tttaagtgat gtgtaaattt atgaatgatt ttgtttattt attttgaaga
    62701 acataaacca ttgtattttg tatggatatg gttaacttaa tgtttattat gaaaattaat
    62761 aatcacactt acaataacat caattaataa atggttaatc atttttgata tatcgtaaga
    62821 ataatgtagt ttttaacacc atccctggta tctcaactat ctctataaaa cagcgtgacg
    62881 ctgtcgtcct ctggctctat cccagatgcc gtaaaaacgc cctgcattgc tggcggtata
    62941 ccagacagta tgacgaatat tgcgatgccc aagataatcc tggataagtc gcgtatctat
    63001 tcccatattc gccaaagcaa aaccacacga atggcgtaac atgtgcggat gaatctccag
    63061 tgacaacccg gcattaccac cggaagtcga gataatatgg taaaactgtt gccgagaaag
    63121 cggatttccc ttacgagata aaaataccca ctcgctctca gcatgcgggt acgaagtacg
    63181 gatactcaac cagtttttta aagcctgaac ttctttgttc aatagcggat gcgttgttga
    63241 aaagcctttt tttaatcgat ggatatatat acactttgcc ttaagatcaa tatccgaaat
    63301 cctcaatcga caaatttcac tcgcccggaa accatgaata aaacaaagca aagtcagaca
    63361 ataattacgt gctgcatgag gcccggtatt tgctgcttta aggagtgatt cgatttcact
    63421 atgggtcagg aagttccttt ttttgttatc agccttattc ttcatcgttt ttcccttata
    63481 attacagacg cgcactagct gtgctgggtt agcaataatc caacattttt atcctgatta
    63541 tgtttataaa agcgaacgtt tgcttaataa ctaacgtaag tggaccagtt cttctgagtg
    63601 aacttaaatg gagtagcaac agttaattat aataacgttg ccatacaaac gccatgctct
    63661 tgcatgctat gtacctttat atatttatca atcggatacg aatctaataa taaccctcat
    63721 cattaatgca actaatctta tctatatatc atgtgatatg ttctgtaaca agtaaaatca
    63781 acataaaaaa ttcagcaaaa tttaatattc gttcaactag ccgtctcaca tatgacatta
    63841 ttagtcaact ccatttgcca caaaatggct atttcatgga gcgcagtaat ttcttagtgg
    63901 ctattatcat gctaattctt aggtccctag cgattattcc tggctgattc gtcagcttac
    63961 gccatgagat tgttacattc ctttttatcc tggagatatg gggaacgcag gagatgattt
    64021 tttcatctac attcgttggt aaatatagct ttggtcacta cgacttaaac cttcctcaat
    64081 cacagccatt cgccgggcga tacggcaaac aaactaagtg aaacatcatc ctgaaaacac
    64141 caacatcaac aagcctctcc agatcgactt cagaagtgac cagttacaag ccacaaacca
    64201 ggaaacatat ttcatgatga gaattatgct cagcctgatg gcgggtaaac actgattttc
    64261 accacgctca ctgagttaac ttgcaaatct ttccttgttt atactttaaa taaattactt
    64321 ataaaacaga taattaaaca acaatctttg gtttgtgaag gcctccgcaa gtgttcgtct
    64381 ggcttcacat ggcatcttct tcttagaaaa atatcgacat attttgtgac acgaattgca
    64441 aatctggttt tgttgtatgg attgcgtgat ttttgatctg gtataacagg tataaaggtg
    64501 caccaagata gtcaatgaga cagggcatct cgcaatctat ggcaaacatc acttcagttc
    64561 tttctcatcg ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa tgagatcaac
    64621 agacattcta ttttatgact ctgggtaaaa tggattgagt aagtgatata gcttacgaac
    64681 attcaaatca attaaacatc agaagagatt ttatactcag gtatttaatc tggatctctg
    64741 tttatttaaa taatgtgaaa agagattttt cacaggagac attatacaaa aaaatataaa
    64801 atacagctac cggttgccaa agacactata agcgtggcaa aaaaatatta cacaacataa
    64861 atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg agtgaaattt
    64921 atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc cccattaatt
    64981 tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca cgcctatgag
    65041 actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat ggaaagtaat
    65101 acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt
    65161 gaagttaatt acgcgattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg
    65221 cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca
    65281 aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac
    65341 ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat
    65401 attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca gaacgattat
    65461 cgctatgcaa accataagaa atgggcgacg gaaaatgcat ttgttctaca ataccatatg
    65521 acgcccgata ttacgccata catagaatat gactacctgg accgtcaggg tgtttacaac
    65581 ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag
    65641 acaggagaca gtcacaatga ataaaacaat aacggcgctt gctatcatga tggcttcatt
    65701 tgccgcaaac gcgtctgtat tacctgaaac tcctgtgcca tttaaaagtg gtaccggagc
    65761 aattgataac gacactgtct acattggttt aggtagcgca ggtacggcat ggtacaagct
    65821 ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg gtggaccaag
    65881 agatcaagca acctcggcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa
    65941 aaatagcaag ggcttgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa
    66001 tagttgggtt aaattgatgt cgcatgcacc gatgggcatg gcgggtcatg tgacttttgt
    66061 acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt
    66121 tgaagatctc aacgaggctg gaaaagattc agcaactata gacaagatca atgcccatta
    66181 ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt ttgatccctc
    66241 aacacagcaa tggagttacg ctggcgaatc tccctggtac ggaacggctg gtgcggcgct
    66301 tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac
    66361 ggatgccgta tttgaacttg atttcaccgg taataattta aaatggaata agcttgctcc
    66421 cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg attctcttat
    66481 atttgccgga ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta
    66541 tgcgcatgaa ggcctaaaaa aatcatatag cactgatatt catctttggc ataacgggaa
    66601 atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa
    66661 taatagtcta ttgattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt
    66721 tttgatctct gtgaaggata ataaagtcac agtacaaaat taacgcttca gggccccggt
    66781 aaaggggccc tgctatcaat ttgtcatttc aattacgcga aatttatatg aacgcaataa
    66841 tatcgcccga ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg
    66901 gcgaaggact gccattaccg gacagggaag atgcgcctca tcccagaatt aaacaattag
    66961 cgagatttgc gcatacgcat cccggaggcc cgtcatgtca ctttaacgac attattccac
    67021 tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga
    67081 atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg aaattactac
    67141 cctttattcc tgataatgca ggggttctca tcgttccatg ttgccgtggc ggatcggctt
    67201 ttaccgcggg cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc
    67261 gttggggaac ggatactccg ctgtaccagg atttagtcag cagaacgcga gccgcactgg
    67321 caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa
    67381 tgaccagtga ctacgcgtca caccctcaac actttaatca tatgattgaa gcctttcgta
    67441 gggatctaaa acaataccat tctcagctta ataatattac tgacgcgccg tggttttgcg
    67501 gcgataccac ctggtactgg aaagaaaatt tccctcatgc gtatgaagtt atttatggca
    67561 attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa
    67621 gaggactgac gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt
    67681 cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcgcgg
    67741 cagcccgtcg ggggattatt tctgacaggt ttgtagaagc aattttgcag ttttggggcg
    67801 aaaagtgagc gttcattaat ttgatttatt cgcatccatc aacgctttta actcctccgg
    67861 cgtaaacggt ggttagcgtc ggtgcagcat cgcatggcaa ttagggtaca caggcagcaa
    67921 aaaaacaaat gagatcaaag taatatatct tcacttgcag gcttgaacag ccgaaaattt
    67981 gcgagcatga tcgcaattat ttcactgcca gggatatcaa tggcttccag taaatcgcta
    68041 cagcaggcaa ttgccaatat aaaaatctgg cacaagggtg aacagcgcgc gccgcataag
    68101 ccattgttat tgctatacgt attagcggga tacctgaatg gacatccacg ccttttcgat
    68161 tattgtagtg gaacagtcta cgaggatgca gtttgaagtg agtaagtaaa ggttgctgga
    68221 aacgatcaat gccgccatac aatgatattt gaggatcacc aatgagtaat aaaaactacg
    68281 aaagtcaccg caaagcaata gttagtaaag gtataccacc cactctatta aataggctca
    68341 ccaattcgga tgttcaggtg atcaatacct tcttaacacg agtgagcaaa ctggaactat
    68401 ctcaacaaga gaaagactgg atcataaaaa tcatctctat ggtttagaga atttcagcct
    68461 tgtagccttt caagggtttt gtgtgggtac aaacattatc gaatcgggat atagccgcag
    68521 ctgtagctgt atccacatca gtaagttgat gtaggttgac ttaggttgag cgaaaaatag
    68581 cgaaaagcct tgtgtggcgc ggatttcagt cataaaaaaa cgtccgttga cgtctattga
    68641 tgttccgatg gtgcgaaggt gcgaaggtgc gaaggccgga ctcgaacatg tgacttaacc
    68701 tcatgattta aaattgttaa taataaacaa tatcatctta taccaacaca gataccaaca
    68761 cgaaaaatca ctggttttcg gcatcgaacc aggacaatca gctcctgatt gagataccga
    68821 gctctgttac tcgtcatctt cctcgacagg cttcattgca tcaagtagta ctcgcaccat
    68881 cgcgatagaa ccaatccgga taaaagcatc ttctccatct atatctgtta actgatatcg
    68941 ctcacttgat gccggataag tgtgtcgatt tgcagctgtg tactcttttg tacagccaga
    69001 atgtggctga ctgaggtgga gcagataatg tccccgttga ccacttccgg acgggaggct
    69061 gtgatttcag acataattga tattcctttt tgcagataaa tgaaaacgcc agccctgttc
    69121 aggctggcgt cagtggaatg aagtatggtg agtaatattt atcggttctg gtgcagaagc
    69181 tgcagttggc gcagggtacg gataccctcc ctggcctgtt cgatacaggg caacagtgct
    69241 gccgaatccg ttttatcctc atcgttgtca aagataattc ccgattcgca gtcgatattg
    69301 tcctgcagcc acacaatcag aatatccagc gccgtttctg tggttaatga tttcatcctg
    69361 tgtcttttgt cctggttacc agttgaaagt gggtaaatcc tgcagacatt cagcaccggc
    69421 atccagatga atgagactga cgccataacg ccggatgagt gtgacaacca gacggcggaa
    69481 cgttctggac agccccaggc gtttaagccg cagaaccggg tatgaccacg catcggtacg
    69541 taacagatat ccgctacctg tgaaatgaat ccattctgat tcgccggtgt cattcgtctg
    69601 atttgacagc gagtacaacc aggcattgtc cgtttccgtg atatgtgcgg tactgcagcg
    69661 tatgccggta agggccgcag atggtggcgt gcgctctgct gatgtccggt cagattcatc
    69721 aacaacacga agggagtaac cgttatcgca gaccacgtta atcagttcag cgagagtaac
    69781 accatcgacg tcaacgacaa tgcgccccat gttcagtgcc tgcacgttaa tgctgtcggc
    69841 ttccagcgtc agggccagtt tcattgtttc gcccccggat gtttacccag ggtaatgtta
    69901 tttaccgttc tgtaattgtc gcgggccatc aggccggttg cccggcgggc ccggaggata
    69961 tcaatactgt ttattaactg agaacgagta cagacgctga atcccggctg gtcggtacgc
    70021 accagtgcgt atttttcaac gagaaagttc accgcatcac acagtgaaat gcctgcctca
    70081 atatgctgct caatcacacg ttcatcagcg aacggtgtgt cattcagtgt gaggccgtaa
    70141 tgctggtcca gcagtcgggt gagcagtgtc tgccagatgg tgacgggaga ctggcaatgc
    70201 aatgcctccc gtacgtgtgt atcgggtaat gtgttcatac tgaagatttt cctgatatgc
    70261 agagatgaaa aatggaaaag tggcgtggtg aaagcaccag gccggagaag aaggttattc
    70321 tggagagtta ctttttcgtt tcgggcgtcg gataaacagc catataaacg tagccacaac
    70381 tgccgagggt gtcggcttcg caggtcagcc tttttgcata cagcgtgacg gtatgctgat
    70441 ggcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa
    70501 aggcctggtc cagatggtac gcatccgcgt cgctgaaccg gcctctgata ccggcgcggt
    70561 ctgcaaggta atgtaaccgg ttaccctcct gcaccagacg tgccccaaaa cagggcgtca
    70621 cggtgcaggg cagtccccac caggtgcggt cgttattgtc gtcgggaagc gttgtcccgg
    70681 ggagtgtgtc tgacacgata aaatcctcac agaaaattgg ttaagaatgg tccggtggtg
    70741 gcgataattc tgctcttctg aatgcccttc cagactcagt tcagggtgac gctcatcagc
    70801 cggacatacg gaccaaaact gtccttacgg cgttctgcaa acacagccag cacaccggga
    70861 atatcctgta cttcacggcc ggtatatgta tccgtgctac cgtgccagcg gtatttaccg
    70921 gtacagaacg gaaaaagacg ggatgcagga tgctgacggt gaatacgcat ggcttcacca
    70981 cgggtgatga ttttcatgag aatagtcctc ctgaatgtga aagtaagaaa cagctgagaa
    71041 agagaaactg aagtgatatg aaggtgattg tgacggtgac gggttaaagc agaccgtgtt
    71101 cggcaaagga gaatacctgg ctgccaccga ctatcagatg gtccggcacc cggatatcca
    71161 ccagggccag cgcctgtacc agacgttcag tgataagccg gtctgcctta ctgggggtga
    71221 cttcaccgga cgggtgattg tgcgccagca ccacggcagc ggcattgtgg tacagggcac
    71281 gtttaatcac ctcccgggga tggacttctg tgcggttgat ggtgcctgtg aagagggttt
    71341 cacccgcaat cagctgattc tggttgttca gatacagcac ccggaactct tcccgctcca
    71401 gtcctgccat gttcagaatc agccattccc gtgccgcatg cgtggaggtg aaggccacgc
    71461 cgggttcatg aagatggcgg tccagggttt tcagggcccg ctgaatgagg ctgcgctcgc
    71521 cgggcgtcat ctctccgggc agaaaggaaa gctgttgcat tgt
//
LOCUS       DAHBHC010000016        67383 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHBHC010000016 DAHBHC010000000
VERSION     DAHBHC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.016"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..358
                     /gene="yrbL"
                     /locus_tag="M2A35_001386"
     CDS             <1..358
                     /gene="yrbL"
                     /locus_tag="M2A35_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YrbL"
                     /protein_id="HCB7197706.1"
                     /translation="TLTEFAEQCRYEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNI
                     LCHRISESEVIPVVCDNIGESTLIPLATWSKWCCLRKQERLWKRFIAQPALAIALQKD
                     LQPRESKTLALTSREA"
     gene            complement(355..1083)
                     /gene="mtgA"
                     /locus_tag="M2A35_001387"
     CDS             complement(355..1083)
                     /gene="mtgA"
                     /locus_tag="M2A35_001387"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCB7197707.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(1080..1733)
                     /gene="elbB"
                     /locus_tag="M2A35_001388"
     CDS             complement(1080..1733)
                     /gene="elbB"
                     /locus_tag="M2A35_001388"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCB7197708.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(1963..4299)
                     /gene="arcB"
                     /locus_tag="M2A35_001389"
     CDS             complement(1963..4299)
                     /gene="arcB"
                     /locus_tag="M2A35_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCB7197709.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(4395..5324)
                     /locus_tag="M2A35_001390"
     CDS             complement(4395..5324)
                     /locus_tag="M2A35_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCB7197710.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            5999..10459
                     /gene="gltB"
                     /locus_tag="M2A35_001391"
     CDS             5999..10459
                     /gene="gltB"
                     /locus_tag="M2A35_001391"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCB7197711.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            10472..11890
                     /gene="gltD"
                     /locus_tag="M2A35_001392"
     CDS             10472..11890
                     /gene="gltD"
                     /locus_tag="M2A35_001392"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCB7197712.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLAHEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTSMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEDNMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            12074..13201
                     /locus_tag="M2A35_001393"
     CDS             12074..13201
                     /locus_tag="M2A35_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCB7197713.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(13261..13725)
                     /locus_tag="M2A35_001394"
     CDS             complement(13261..13725)
                     /locus_tag="M2A35_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCB7197714.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(13722..14597)
                     /gene="nanK"
                     /locus_tag="M2A35_001395"
     CDS             complement(13722..14597)
                     /gene="nanK"
                     /locus_tag="M2A35_001395"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCB7197715.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(14594..15283)
                     /locus_tag="M2A35_001396"
     CDS             complement(14594..15283)
                     /locus_tag="M2A35_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCB7197716.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(15331..16821)
                     /gene="nanT"
                     /locus_tag="M2A35_001397"
     CDS             complement(15331..16821)
                     /gene="nanT"
                     /locus_tag="M2A35_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCB7197717.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(16930..17823)
                     /gene="nanA"
                     /locus_tag="M2A35_001398"
     CDS             complement(16930..17823)
                     /gene="nanA"
                     /locus_tag="M2A35_001398"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCB7197718.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(17945..18736)
                     /gene="nanR"
                     /locus_tag="M2A35_001399"
     CDS             complement(17945..18736)
                     /gene="nanR"
                     /locus_tag="M2A35_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCB7197719.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGITHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            19116..20480
                     /gene="dcuC"
                     /locus_tag="M2A35_001400"
                     /pseudo
     CDS             19116..20480
                     /gene="dcuC"
                     /locus_tag="M2A35_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(20523..21020)
                     /gene="sspB"
                     /locus_tag="M2A35_001401"
     CDS             complement(20523..21020)
                     /gene="sspB"
                     /locus_tag="M2A35_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCB7197720.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(21026..21664)
                     /gene="sspA"
                     /locus_tag="M2A35_001402"
     CDS             complement(21026..21664)
                     /gene="sspA"
                     /locus_tag="M2A35_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCB7197721.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(22059..22451)
                     /gene="rpsI"
                     /locus_tag="M2A35_001403"
     CDS             complement(22059..22451)
                     /gene="rpsI"
                     /locus_tag="M2A35_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCB7197722.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(22467..22895)
                     /gene="rplM"
                     /locus_tag="M2A35_001404"
     CDS             complement(22467..22895)
                     /gene="rplM"
                     /locus_tag="M2A35_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCB7197723.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(23114..24241)
                     /gene="zapE"
                     /locus_tag="M2A35_001405"
     CDS             complement(23114..24241)
                     /gene="zapE"
                     /locus_tag="M2A35_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCB7197724.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDESRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            24435..24833
                     /locus_tag="M2A35_001406"
     CDS             24435..24833
                     /locus_tag="M2A35_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCB7197725.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            24987..26354
                     /gene="degQ"
                     /locus_tag="M2A35_001407"
     CDS             24987..26354
                     /gene="degQ"
                     /locus_tag="M2A35_001407"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCB7197726.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            26444..27511
                     /gene="degS"
                     /locus_tag="M2A35_001408"
     CDS             26444..27511
                     /gene="degS"
                     /locus_tag="M2A35_001408"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCB7197727.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(27573..28511)
                     /gene="mdh"
                     /locus_tag="M2A35_001409"
     CDS             complement(27573..28511)
                     /gene="mdh"
                     /locus_tag="M2A35_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCB7197728.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            28946..29416
                     /gene="argR"
                     /locus_tag="M2A35_001410"
     CDS             28946..29416
                     /gene="argR"
                     /locus_tag="M2A35_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCB7197729.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            29781..30044
                     /gene="yhcN"
                     /locus_tag="M2A35_001411"
     CDS             29781..30044
                     /gene="yhcN"
                     /locus_tag="M2A35_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCB7197730.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(30099..30371)
                     /locus_tag="M2A35_001412"
     CDS             complement(30099..30371)
                     /locus_tag="M2A35_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCB7197731.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(30463..32430)
                     /gene="aaeB"
                     /locus_tag="M2A35_001413"
     CDS             complement(30463..32430)
                     /gene="aaeB"
                     /locus_tag="M2A35_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCB7197732.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(32436..33368)
                     /gene="aaeA"
                     /locus_tag="M2A35_001414"
     CDS             complement(32436..33368)
                     /gene="aaeA"
                     /locus_tag="M2A35_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCB7197733.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(33376..33579)
                     /locus_tag="M2A35_001415"
     CDS             complement(33376..33579)
                     /locus_tag="M2A35_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCB7197734.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            33762..34691
                     /gene="aaeR"
                     /locus_tag="M2A35_001416"
     CDS             33762..34691
                     /gene="aaeR"
                     /locus_tag="M2A35_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCB7197735.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(34819..36264)
                     /gene="tldD"
                     /locus_tag="M2A35_001417"
     CDS             complement(34819..36264)
                     /gene="tldD"
                     /locus_tag="M2A35_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCB7197736.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(36420..40220)
                     /gene="yhdP"
                     /locus_tag="M2A35_001418"
     CDS             complement(36420..40220)
                     /gene="yhdP"
                     /locus_tag="M2A35_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCB7197737.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(40288..41757)
                     /gene="rng"
                     /locus_tag="M2A35_001419"
     CDS             complement(40288..41757)
                     /gene="rng"
                     /locus_tag="M2A35_001419"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCB7197738.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(41747..42340)
                     /gene="yhdE"
                     /locus_tag="M2A35_001420"
     CDS             complement(41747..42340)
                     /gene="yhdE"
                     /locus_tag="M2A35_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCB7197739.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(42349..42837)
                     /gene="mreD"
                     /locus_tag="M2A35_001421"
     CDS             complement(42349..42837)
                     /gene="mreD"
                     /locus_tag="M2A35_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCB7197740.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(42837..43940)
                     /gene="mreC"
                     /locus_tag="M2A35_001422"
     CDS             complement(42837..43940)
                     /gene="mreC"
                     /locus_tag="M2A35_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCB7197741.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(44006..45049)
                     /gene="mreB"
                     /locus_tag="M2A35_001423"
     CDS             complement(44006..45049)
                     /gene="mreB"
                     /locus_tag="M2A35_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCB7197742.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(45354..47294)
                     /gene="csrD"
                     /locus_tag="M2A35_001424"
     CDS             complement(45354..47294)
                     /gene="csrD"
                     /locus_tag="M2A35_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCB7197743.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            47446..48420
                     /locus_tag="M2A35_001425"
     CDS             47446..48420
                     /locus_tag="M2A35_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCB7197744.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            49398..49868
                     /gene="accB"
                     /locus_tag="M2A35_001426"
                     /gene_synonym="fabE"
     CDS             49398..49868
                     /gene="accB"
                     /locus_tag="M2A35_001426"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCB7197745.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            49879..51228
                     /gene="accC"
                     /locus_tag="M2A35_001427"
     CDS             49879..51228
                     /gene="accC"
                     /locus_tag="M2A35_001427"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCB7197746.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            51337..51579
                     /locus_tag="M2A35_001428"
     CDS             51337..51579
                     /locus_tag="M2A35_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCB7197747.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            51569..53020
                     /gene="panF"
                     /locus_tag="M2A35_001429"
     CDS             51569..53020
                     /gene="panF"
                     /locus_tag="M2A35_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCB7197748.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            53032..53913
                     /gene="prmA"
                     /locus_tag="M2A35_001430"
     CDS             53032..53913
                     /gene="prmA"
                     /locus_tag="M2A35_001430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCB7197749.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            54242..55207
                     /gene="dusB"
                     /locus_tag="M2A35_001431"
     CDS             54242..55207
                     /gene="dusB"
                     /locus_tag="M2A35_001431"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCB7197750.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            55233..55529
                     /gene="fis"
                     /locus_tag="M2A35_001432"
     CDS             55233..55529
                     /gene="fis"
                     /locus_tag="M2A35_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCB7197751.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            55615..56499
                     /gene="yhdJ"
                     /locus_tag="M2A35_001433"
     CDS             55615..56499
                     /gene="yhdJ"
                     /locus_tag="M2A35_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCB7197752.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            56583..56762
                     /locus_tag="M2A35_001434"
     CDS             56583..56762
                     /locus_tag="M2A35_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCB7197753.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(56765..57427)
                     /gene="acrS"
                     /locus_tag="M2A35_001435"
     CDS             complement(56765..57427)
                     /gene="acrS"
                     /locus_tag="M2A35_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCB7197754.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            57826..58983
                     /gene="acrE"
                     /locus_tag="M2A35_001436"
     CDS             57826..58983
                     /gene="acrE"
                     /locus_tag="M2A35_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCB7197755.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            58995..62099
                     /gene="acrF"
                     /locus_tag="M2A35_001437"
     CDS             58995..62099
                     /gene="acrF"
                     /locus_tag="M2A35_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCB7197756.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            62352..62573
                     /locus_tag="M2A35_001438"
     CDS             62352..62573
                     /locus_tag="M2A35_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197757.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            63004..64023
                     /locus_tag="M2A35_001439"
     CDS             63004..64023
                     /locus_tag="M2A35_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7197758.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLSRN
                     TTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELNVA
                     DYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVLPE
                     VISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAHLL
                     GKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGIQY
                     APPVR"
     gene            64091..65272
                     /locus_tag="M2A35_001440"
     CDS             64091..65272
                     /locus_tag="M2A35_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCB7197759.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            65282..66385
                     /locus_tag="M2A35_001441"
     CDS             65282..66385
                     /locus_tag="M2A35_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCB7197760.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            66393..67151
                     /locus_tag="M2A35_001442"
     CDS             66393..67151
                     /locus_tag="M2A35_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCB7197761.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
BASE COUNT        16362 a        17683 c        16996 g        16342 t
ORIGIN      
        1 tacgctgacc gaatttgctg aacaatgccg ttacgaagaa gacattgcac aactgcgcca
       61 gctactgaaa cagctgaagc gttatttgca ggataaccgt atcgtgacga tgtcgctgaa
      121 gccgcagaat atcctctgtc accgcatcag tgagtcagag gttatcccgg tcgtctgtga
      181 caacattggt gaaagcacat tgattccact ggcgacctgg tcgaagtggt gttgtctgcg
      241 taagcaagag cggttatgga aacgatttat cgcccaaccg gcgctggcta ttgccctgca
      301 gaaagatctg caacctcgtg agagcaaaac gctggccctc acttcgcgcg aagcttaatc
      361 cagctggtgt tgttgcataa acggctcacc gcctaactga tacatctgcc gtaaaatcca
      421 cgcctgacgg ctacgcacgt agcccgatgg cgaggagact ttgaaacgaa gtggattagg
      481 taatacagct gccagtaatg cagcttccga ccgggtaagt ttgctcgcgg gtttgtggaa
      541 ataacgttgt gccgcagctt cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag
      601 gtaaaccgtc aggatacgct ttttgctcca gactgtttct atccccagcg ttaatccagc
      661 ttccagccct tttcgtaccc agctacgccc atcccataaa aagagatttt tggccgtctg
      721 ctgagaaatc gttgaagcac cgcgaatacg gttttcattg cgctcgttgt gcgccagggc
      781 tttctcaatg gaagcgacat caaagcccca gtgctcagga aatttctgat cttctgcggc
      841 aatcaccgcc agtcccatcc acggcgagat ttgatccata ctgacccagt cagaatgtgc
      901 cacgtaacga aaattgccat gcagccaggc gctgacctgt cgctcgacca ttaccgctga
      961 gaagggaaca ggcgcaacac taaacaacgc gatgcccccg ccccagaaaa cggcgaggac
     1021 aaccattaac cgcaaaagaa aacggcgaac gaaactaaac accgttaagc ggcttttact
     1081 cattcagcca gaaccagcac gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca
     1141 atgttctgcg ccagcatata tgctggggtg gtgacaatct tattgtcttc atcaaccacg
     1201 atatcatcga caggacaggg cacatgctcc gcgcccatct cttccagcac ttctgcggta
     1261 tcgatatcag taccgatggt caaacgcagc gggaaatcga aaattttcgg cagcatcgcc
     1321 ggggcaatac acataaaacc aagcggtttt ccggcctgat gcatcgcttg tgccagcgcc
     1381 tttaattcac ggtcaacggt gcattcgcta ccaagagtgg caaaattgct taaattcttc
     1441 gccgcgccaa acccccccgg cacaatcaac gcatccagtt cagcggcatc ggcctgggcc
     1501 agaggacgga tttcaccgcg cgttattcgt gccgcttcaa tcagcacgtt gcgcgtttcc
     1561 gtcatcgctt cgccagttaa atggttgata acatcaacct gctgcttatc cggtgcaaag
     1621 cagaccgcct gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt
     1681 tcagaaccgt catagacgcc gcatccgctc agaattacgc caattttctt cattgtgatc
     1741 atcctttcgc aattgactga aacacattaa tttttttaat aaaaatggta cgcatcacac
     1801 atttaactga ttcatgtaac aaatcattta agttttgcta tcttaactgc gtgcggcctg
     1861 aaaaacagtg ctgtgccctt gtaactcatc ataataattt acggcgcagc caagatttcc
     1921 ctggtgttgg cgcagtattc gcgcaccccg gtctagccgg ggtcattttt tagtggcttt
     1981 tgccacccac gctttcagca cttctacgtc gtgacgccac tcttctttca tctcttcaat
     2041 ccattcaccg acgttatctt cccaggcagg aaggtccgga gactgaattt gctgacccag
     2101 ctgttgcaga tggcgtaacc ccactgaccc cgccgcacct ttaattttat gtccttcctc
     2161 tacaatgcct tttttatcct gcgccgtcag attcgactcc agcacgctga catagcccgg
     2221 catcatcttc tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta caagttcgag
     2281 atactgttcc agcatgggaa tatcgagcaa tgcttctgat ttactgttct cttctgtcgt
     2341 caccgtactc tcctcatcat cctgggtatc ccagaatttc ttgatcatcg cggttagcgc
     2401 cggaaccgaa agcggcttac tcagcacatc atccattcca gcattgaggt actcttgttt
     2461 gtctttcagc acgttagcgg ttaaggccac cagcggcggt aaatcctcgc gcggataacg
     2521 cttcgtcagt tcacgagaga tatccagccc ggtcatatct ggcaactgaa tatccagcaa
     2581 caccaggtcg tattcgcccg gtttaaacat ctccagcgcc gccttgccgg tcatggcgac
     2641 atcaacgctg ttacctaatt tttccagcac agaacgcgca acaatcacgt tcagttcaat
     2701 gtcttccacc agcagcacat tcagcgccgg taaaggcata tcgtcttcat caaacgcatc
     2761 atcgacctct tctgctaccg acggtgcgtg gatcgtcaac gtaaaggttg aacctttgcc
     2821 ctgttcgctg gtaaccgtaa tatcgccgcc catatttttc gccagacgac gagaaacggc
     2881 cagaccaata ccggtgccgg tggcaggttt accgccatga ctgtctttca cctggtaata
     2941 catggcgaaa attttatcca gctcatcctg cggaatgccg ataccagagt cttccacttc
     3001 aaaatgcagc atatcgcctt catcgtagcg cacgcgcacg gtaacctggc cttgctgggt
     3061 gaatttgacg gcgttactga tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt
     3121 aatgacctga tgcggtaatg gcagcgtcgg ctccaggtta aagcgcaatc ctttttgttg
     3181 cgcctgcaat gcggagagat tttccagatc ggcaaggaag ctggtgaaat caaccggttg
     3241 attatcaagc tggaccttgc gccgttccat cttatccatg tcgataatat cgttaaagat
     3301 attccccagc gtgacggccg aaacatggat ggtcttgaga tatttttcct gctcggcggt
     3361 gagttcggta tccagcagaa tacggctcag gccgacgata ccgttcagcg gtgtacgcaa
     3421 ttcgtgactg atggtggaaa taaacgtcgt tttgtcgcgg ctggcccgtt caagcgcatc
     3481 ctgataccgc ttacgctcgg taatgtcgcg accaaagccc atcaaaccgt gacgtttacc
     3541 cacgcggtcg tagtacggca ctttacggat ttcaaagcag gctttgcgcc cgtccgggta
     3601 atccagccac tgttcatagg tcagtgacac attatgacgg aacacttttt catcggtttc
     3661 aatgactttt gcggcggctt ccggtgagta aacatcagca ggtttcaggt gaaccagttg
     3721 tttttcgctt tttccggtca gcagctccat cgcgcggtta cagccggaaa actctttatc
     3781 ttcgttacga taaaaaacca ggtcgggtga agcatcaagg aaggaacgta agaatgagga
     3841 ttgctgctcg agctgaattt gtgtctcttc gcgctctttg atttcaattt tcagttggcc
     3901 gaaggtttcc tgcagttctg cttccgcttt ttcacgaacg gcaatttcct gatttagctg
     3961 ggcaatatta tcttttaact gaacgttgag gctcaaatcg cgctcgcgca tctcctccag
     4021 tttttgcacc agccgtgaca gacgttgtcg tgactcctcc agttgctcga cgaccaccga
     4081 tagaaagtag accgcccacg gcgtaatcag caaaccaaag aagatagaac gaataacatc
     4141 aatgctttcg acctgaccat gcagcaccat ggttaccgcc atttgtacca caatggcaag
     4201 aacgacgagg gccagcgcca gcaacattga gaagcgcacc agacctaact tcatcatcag
     4261 gtcaacataa tactgcgcca gcagacgaat ttgcttcatt agggaattcc ttcacgacaa
     4321 cctgccccaa taatacccaa ttctgacagt tacgttataa attgtgtgag aaatgcggaa
     4381 ttcccctcaa cgacctactc cgttggaggt atccacggac gttccagcgc cgatccctgt
     4441 acgccatgtt cattcagata gcgatccagc tcgaccattc ccgtccatcg attttcgcac
     4501 cacagaggag caagcagcgt cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact
     4561 tccggtggcg tatggcgaat catttctccg gcagtgagcg tgtaatcctc cagttcaata
     4621 ccgttcaaac gccccgcttc ccaggctttc gccataatgc tgcctttcac aatatgcagc
     4681 ggatgcagct ttatgccgtc cacgccggtt tcaaccactc gctccagcgt ttgcaaacat
     4741 tcagcctgcc cttcgccagg caggccgaca attaagtggg aacacacctt cagcccgcgc
     4801 tgacgtgcca gctgggttgt acgctgataa caggcaaaat catggccgcg gttgatgcga
     4861 tgcagtgttt tgtcgtgggc ggtttgtagc cccagctcca gccacacttc gtaaccctgg
     4921 tccttatatt cgcaaagcag atccagcacc gcatccggca cgcagtccgg gcgggtacca
     4981 acacacaaac cgacaatatt ggcctggctc accgcctgct gatacataga acgcagcacc
     5041 tgaacttccg caaaggtgct ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg
     5101 ttaactaaat tcgcctggtg cgccagttgc tcggcaatgg aacgatgctg ctgcgcttca
     5161 tcggcaaacg aggcaacatt acagaatgtg cagccaccac gcccgatggt accgtcacgg
     5221 ttagggcagc taaaaccgcc gtgtagcgtc agcttatgca ccttttgccc ataacgacgg
     5281 gtgagatcac caccaaacat attgactaat ttctgtaact gcataatctg atagaccgcg
     5341 ccttgaaaag aggccaaagc ctgccatttt tagctcaatt cggcgatgac ctggatcaat
     5401 cgtcccggcc tgcttttatc aactgcataa tcaatcaaaa ttaccgaaat ttcatgcata
     5461 atcacataaa tcacttttgc ttatcttgtg tcagattttt ttatctcctt atggatttta
     5521 ggcaaaaaca gtagcatgaa acgtcattac caattaaggc agtataaaat gctggttttc
     5581 tcgtcagttc aaggcaggat aagggttaac acgcctttat gacagtcagg aattgactgt
     5641 ttctctaacg acttcccttt tagccttaaa gataaaatcc attttaattt cagtaattta
     5701 ataaagaatt ttgcgctaaa gcacatttct gtaccaataa gcttgccatt tgacctgtat
     5761 cagctttccc gataagttgg aaatccgctg gaagctttct ggatgagcag cctgctcatc
     5821 atatttatgc agtaattgag atcccctctt caccgtatta accgatgcga aaaggacaac
     5881 aagggggcga atgcgaggcg cgcgtatgac acgcaaaccc cgtcgccacg ctctttctgt
     5941 gcccgtgcgc agcggttcgg aagtggggtt cccgcagagc ctgggggagg ttcacgatat
     6001 gttgtacgat aaatcccttg agagggataa ctgtggtttc ggcctgatcg cccacataga
     6061 aggcgaacct agccacaagg tagtgcgtac tgcaatacac gcactggccc gcatgcagca
     6121 ccgtggcgcg attctcgccg atggtaaaac cggcgacggt tgcggcttgc tgttacaaaa
     6181 accggatcgc ttttttcgca tcgttgcgca ggagcgcggc tggcgtttag caaaaaacta
     6241 cgccgtcggg atgctattcc tgaataaaga tcctgaactc gccgctgccg cacgccgcat
     6301 cgttgaagaa gaactgcaac gcgaaacctt gtcgattgtg ggctggcgtg atgtccccac
     6361 taacgaaggc gtgctgggtg aaatcgccct ctcctctctg ccacgcattg agcaaatttt
     6421 tgtgaacgcc ccggcaggct ggcgtccgcg cgatatggag cgccgtctgt ttatcgcccg
     6481 ccgccgcatt gaaaagcgtc tcgaagccga caaagacttc tacgtctgta gcctgtcgaa
     6541 tctggtgaac atctataaag gtctgtgtat gccggcggat ctgccgcgct tttatctgga
     6601 tcttgcggac ctgcgtctgg aatcggccat ctgcctgttc caccagcgct tctccactaa
     6661 caccgtaccg cgctggccgc tggcgcagcc gttccgctac ctggcgcata acggtgaaat
     6721 caacaccatc accggtaacc gccaatgggc gcgtgcgcgt acctataaat tccagacgcc
     6781 gcttatccct gatctgcacg acgccgcacc gttcgtcaat gaaaccggct ctgactccag
     6841 ttcgatggat aacatgctgg aactgctgct ggcaggcgga atggatatca tccgcgccat
     6901 gcgtctgtta gtgccacccg cctggcagaa caacccggat atggacccgg aactgcgtgc
     6961 cttctttgac tttaactcca tgcatatgga gccgtgggat ggcccggcgg gcatcgtgat
     7021 gtccgacggt cgttttgccg cctgtaacct cgaccgtaac ggtctgcgtc cggcgcgcta
     7081 cgtcatcacc aaagataagc tcatcacctg cgcctctgaa gtcggtatct gggattacca
     7141 gcctgacgaa gtggtcgaaa aaggccgcgt cgggccaggc gaactgatgg ttatcgacac
     7201 ccgcagtggg cgtattctgc actcggcaga aaccgatgac gatctgaaaa gccgccatcc
     7261 atataaagag tggatggaga aaaacgtccg ccgactggta ccgtttgaag atctgcccga
     7321 tgaagaagtg ggtagccgcg aactggacga cgacacgctc gccagctatc agaaacagtt
     7381 taactacagc gcggaagagc tggactccgt aattcgcgta ctgggcgaaa acggtcagga
     7441 agcggtcggt tcgatgggcg acgacacccc attcgccgtg ctctccagtc agccgcgcat
     7501 tatttacgac tacttccgcc agcagtttgc ccaggtgact aacccgccaa tcgacccgct
     7561 gcgtgaagcg catgttatgt cgctcgccac cagtatcggt cgtgaaatga acgtcttttg
     7621 cgaagcagag ggccaggcgc accgtttaag ctttaaatcg ccgattctgc tctactccga
     7681 tttcaaacag ctcacgacga tgaaagagga gcactaccgc gcagatacgc tggatatcac
     7741 ctttgacgtc actaaaacca cgctcgaagc gacagtcaaa gagctgtgcg acaaagccga
     7801 aaaaatggta cgtagcggca ccgtgctgct ggtgctctcc gaccggaata tcgctaaaga
     7861 tcgcctgccg gttccagccc cgatggcggt tggcgcgatc cagacccgtc tggtcgatca
     7921 aagcctgcgt tgcgatgcca acatcatcgt cgaaaccgcc agcgcccgcg atccgcacca
     7981 cttcgccgtg ttgctgggct tcggcgcgac ggctatttat ccataccttg cctatgaaac
     8041 gctgggccgc ctggtagaca cccatgcgat tgccaaagat tatcgtaccg tgatgctcaa
     8101 ctaccgtaac ggcatcaaca aaggcttgta caaaatcatg tccaaaatgg gcatctccac
     8161 catcgcctct taccgctgct cgaaactgtt tgaagcggtc ggtctgcacg atgatgtagt
     8221 gggcctgtgc ttccaggggg cggtcagccg cattggtggg gcgagctttg aagacttcca
     8281 gcaggatctg ctgaacctgt cgaaacgtgc ctggctggcg cgtaagccca tcagccaggg
     8341 cggcctgctg aaatacgtcc acggcggcga ataccacgct tacaacccgg acgtggtgcg
     8401 cacgctgcaa caggcggtac aaagcggcga gtacagcgac tatcaggaat acgcgaagct
     8461 ggttaatgag cgtccggcaa ccacgctgcg cgatctgctg gcaattacgc cgggtgaaaa
     8521 cgcggtcaac attgctgatg ttgaaccggc aagcgaactg tttaaacgct ttgataccgc
     8581 cgcgatgtct atcggcgcgt taagcccgga agcccacgag gcgctggcgg aagcgatgaa
     8641 tagcatcggc ggtaactcga actccggtga aggcggcgaa gacccggcgc gctatggcac
     8701 taacaaagtg tcgcgcatca agcaggtggc ttctggtcgc ttcggcgtta ctccggcgta
     8761 tctggtcaat gccgacgtca ttcagattaa agtcgcccag ggcgcgaagc cgggcgaagg
     8821 cggtcagttg ccgggcgaca aagtcactcc ttacatcgcc aaactgcgct attcggtgcc
     8881 cggagtgacg ctgatctccc cgccgccgca ccacgatatc tactctatcg aggacttagc
     8941 gcagctcatt ttcgacctca agcaggttaa cccgaaagcg atgatctccg tgaagctggt
     9001 ttccgaaccg ggcgtaggca ccatcgcgac tggcgtggca aaagcttatg ccgatttaat
     9061 caccatcgca ggctatgacg gtggcaccgg cgcaagcccg ctgtcgtcag tgaaatacgc
     9121 aggttgcccg tgggagctgg ggcttgttga aacccagcag gcgctggttg ccaatggtct
     9181 gcgtcataag atccgtttgc aggtcgatgg cggcctgaaa accggtgttg atatcatcaa
     9241 agcggcgatt ctcggcgcag aaagcttcgg cttcggcact ggcccgatgg tggcgctcgg
     9301 ctgtaaatat ctacgtattt gccatctgaa caactgcgca acgggtgtag caactcagga
     9361 tgacaaactg cgtaagaacc actatcacgg cctgccgttc aaggttacca attactttga
     9421 atttattgcc cgtgaaaccc gcgagctgat ggcacagctg ggcgtaacgc gtctggtgga
     9481 tctgattggt cgcaccgacc tgctgaaaga actggacggt ttcaccgcca aacagcaaaa
     9541 actggcgctg tcgaagctgc tggagaccgc cgaaccgcat ccaggtaagg cactctactg
     9601 caccgaaaac aacccaccgt ttgataacgg cctgctgaac gcgcagttgc tgcaacaggc
     9661 gaaaccgttt gtcgatgaac gccagagcaa aaccttctgg ttcgatattc gcaacaccga
     9721 ccgttctgtc ggcgcgtcgc tttcaggcta tatcgcccag acgcacggcg atcaggggct
     9781 ggcagccgat cctatcaaag cgtacttcaa cggcaccgca ggccagagtt tcggtgtgtg
     9841 gaacgcgggc ggcgtggaac tgtacctgac cggggatgcc aacgactatg tcggtaaagg
     9901 catggcgggc ggcttaatcg ccattcgtcc tccggttggt tccgccttcc gcagccatga
     9961 agcaagtatt atcggcaaca cctgcctgta tggcgcgacc ggtggtcgtc tgtatgccgc
    10021 aggccgcgcg ggtgaacgtt tcggcgtgcg gaactccggt gctatcaccg tggtagaagg
    10081 cattggcgac aacggctgtg aatatatgac gggcggtatt gtctgtattc tgggtaaaac
    10141 cggcgttaac ttcggtgcgg gcatgaccgg cggcttcgct tacgttctcg atgaaagcgg
    10201 tgatttccgc aaacgcgtta acccggaact ggtcgaggtc ttaagcgttg acgatctggc
    10261 gatccatgaa gagcatctgc gcggtcttat caccgagcat gttcagcata ctggctctca
    10321 gcgcggtgaa gagattctgg cgaactggtc aaccttcgcc actaaatttg cgctggttaa
    10381 accgaagtcc agtgatgtaa aagcactgct gggtcatcgt agtcgtagcg cagcagagtt
    10441 gcgcgtgcag gcgcagtaag gggtagcaac aatgagtcag aatgtttatc aatttatcga
    10501 cctgcagcgc gttgatccgc caaagaaacc gctgaagatc cgcaaaattg agtttgttga
    10561 aatttacgag ccgttttccg aaggccaggc caaagcgcag gctgaccgct gcctgtcgtg
    10621 cggcaaccca tactgcgagt ggaaatgtcc ggtacacaac tacatcccga actggctgaa
    10681 gctcgcccac gaggggcgta tttttgaagc ggcggaactg tcgcaccaga ccaacaccct
    10741 gccggaagtt tgcggacgag tctgcccgca agaccgtctg tgcgaaggtt cctgcactct
    10801 gaacgatgag tttggcgcgg tgaccatcgg caacattgag cgttatatca atgataaagc
    10861 gttcgaaatg ggctggcgtc cggatatgtc cggcgtgaag caaaccggta aaaaagtggc
    10921 gattatcggc gcaggcccgg caggtctggc gtgtgcggat gtcctgacgc gtaacggcgt
    10981 aaaagccgtt gtcttcgacc gtcatccaga aattggcggg ctgctgacct tcggtattcc
    11041 ggccttcaag ctggaaaaag aggtaatgac gcgtcgccgt gaaatcttca ctagcatggg
    11101 tattgaattc aaactcaata ccgaagtggg ccgcgacgtg cagctggacg atctgctgag
    11161 tgattacgat gccgtcttcc ttggcgtcgg gacttatcag tcaatgcgcg gcgggctgga
    11221 aaacgaagac gccgatggcg tgtacgcagc gctgccgttc cttatcgcca acaccaaaca
    11281 gttaatgggc tttggtgaaa cccgcgacga accgttcgtc agcatggaag gcaaacgcgt
    11341 ggtggtcctt ggcggtggcg acactgcgat ggactgcgtg cgtacgtccg tgcgccaggg
    11401 agcgaagcac gttacctgtg cctatcgtcg tgatgaagat aacatgccgg gttcccgccg
    11461 cgaagtgaaa aacgcgcggg aagaaggcgt agagttcaaa ttcaacgtcc agccactggg
    11521 gattgaagtg aacggtaacg gtaaagtcag cggcgtaaaa atggtacgta ccgaaatggg
    11581 cgaaccggat gccaaaggcc gtcgccgcgc ggagatcgtt gcaggttccg aacatatcgt
    11641 tccggcagat gcagtgatca tggcgtttgg tttccgtcca cacaacatgg aatggctggc
    11701 aaaacacagc gtcgagctgg attcacaagg ccgcatcatc gccccggaag gcagcgacaa
    11761 cgccttccag accagcaacc cgaaaatctt tgctggcggc gatatcgtcc gtggttccga
    11821 tctggtggta accgctattg ccgaaggtcg taaggcggca gacggtatta tgaactggct
    11881 ggaagtttaa gctctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc
    11941 ctttctttct ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg
    12001 gattgttacc ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat
    12061 aaagggacag ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca
    12121 cgacggtatt attcatctgg tcgatagcgc ccggacggaa acggtacgta gcgttaacgc
    12181 gctgatgacc gcgacatact gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg
    12241 cgaggccagg gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg atttaagtct
    12301 aaggtataag cgtgggttct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt
    12361 ttttcaacat gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc
    12421 gcagacacca tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatc
    12481 tttccccctg ccctggtcca cctacgtccg tttactttcc gttaaaaacg ctgacgcccg
    12541 cagcttttat gaaaaagaga cgctccgctg tggctggtct gttcgccagc tagaacggca
    12601 aattgcgacc caattttatg agcgaacact actgtcacat gacaaatcag ccatgctgca
    12661 acaacacgct cctaccgaga cgcatattct tccgcagcag gcgatacgcg atccctttgt
    12721 gctcgaattt ctggaattga aagatgaata ctcagaatcc gattttgagg aggcgctgat
    12781 caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg ttggtcgaca
    12841 gcgaaggtta cgcattgatg acaactggtt tcgtgtcgat ctgctgtttt tccaccgccg
    12901 tttacgctgc ctactaatcg tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg
    12961 acagatgaat atgtatctca actacgccaa agagcactgg acgctaccgg atgaaaatcc
    13021 gcccatcggt ctggttctct gtgcagagaa aggagccgga gaagcacatt atgctctggc
    13081 aggtttgcct aacaccgttc tggcaagcga atataagatg caactacctg atgagaaacg
    13141 actcgcagat gaactcgttc gaacacaggc ggtgctagag gaaggctata gacgccgtta
    13201 atttcagcgg tttcgaattc tgcagacagt gtctgcagaa tctttaaaac gaactaagtt
    13261 ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctctc caggctcgcc
    13321 gacaacgcat cctggtttat gcggttcacc tggcataaac acggcgaaca ttcccggttt
    13381 taagatgatg gtttgctcgt tttcaatggt gctgcaaagc tgataatcat cctcatggtg
    13441 gaactcttca cactggcgcg cagtgcctgc catgccaaac agaatccgtt cctcaccgtt
    13501 taataacagt tggatatcaa tgtattgctc gtgcaattcc gcttttttct cgacgggcga
    13561 ttgagtgtta aacgtcatga cattcataaa gatattgtcg ccctgtaatt cgtaacgacc
    13621 cggcgctttt tcttgcggcc tggcggctaa tgccagcgtt aacgcgtcct gtaacgcagg
    13681 atgtaaccca gcagacggta atgactgtac ttcacccatc atcataattt ttctccctgg
    13741 gccaacagcg cagccccaag taaacctgca tcatggcggt agtgcgccgc cagtaaatca
    13801 acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccagcgccag atacccttct
    13861 gccagaccaa cgctgccacc gaccaccacg cactggcaat cagttgtggc tttaatatca
    13921 gcgatcagcc ttgcaagcgt acgtgcggag cgatggatca gccattgcgc ttgttcgtcg
    13981 ccctgcccgg cgcgtgtaaa aatagttctc gcatccgcgc ccgccagttc accctgcgct
    14041 gccgccgcaa tgccgcgacc agaagcaatc gcttccacgc aacctgtgcg tccacagccg
    14101 cagactggac catgcggatc ggcaagcgta tgcccgatat gccccgccag accgccaggg
    14161 ccggtaagca gtttaccgcc gctcactaca ccgccgccga cgccagtgga aacggtgata
    14221 aacaccatct cggttacatc gccttccagc gcctgatact ctgcccatgc tgcggcctgc
    14281 gcgtcgttaa tggcaatggt cggcaaattg gtaagttgtt ccagcgtttt gactaacgga
    14341 aagtgtagca atccaccgag attatgcgga ttcagcgcca gcaaactgcc gtcgcgaata
    14401 attccggttg aagcgatggc aacccgctgc gcatgagctt gcaacggaga gactaatgcg
    14461 gataaggcat cacgcaaggc ttctggtgtc tggctggctg gcgtaggaag ttcacgacga
    14521 tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa gtttagtacc gccgatatca
    14581 atcgccagtg tggtcatagc accgcctttt tcatcgctgt gttgtaccac tgacaaatgt
    14641 gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat
    14701 ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat cacccgacat ccggcgtcgc
    14761 tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg gtatagccag
    14821 aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg tcttccggcg
    14881 ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc agcgtttcaa
    14941 caggcaccgg acgcgggcgg tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg
    15001 ccagcgcatc gacatcttca atataggccg tgatgcgtac cggagaatcc tccagatcgc
    15061 gtttcacaat tccaataatc ggcacgctca ccaccgcacg cgtggcttgc agatttgcca
    15121 caccttcaat acgaatggcg accgcgcccg cctgctctgc cgctaatgcc atggcggcga
    15181 cgatttcggg tttatcgagc gggctgtcag gaaccggctg gcaggagaca atcaggccac
    15241 cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt cccttagcga
    15301 aaggcccggt acagagaccg ggcaacagga ttaacttttg gttttgacta aatcgttttt
    15361 ggcgctgcca aacggcacgg caccgctgaa tggtttaccg tcgatagcgt catgagtacg
    15421 caacgcttcc gggcgcaacc aacgctgaac gcgagaaggc atatccagcc caatcagcag
    15481 gatcaccacg aacgtcagac tgaacgagag cgatgccagc gcagtaccca gatccagacg
    15541 ttgagcgatc aacgcgccga tgattggggc cagtgcaccg cccaatgcgc caacgttgta
    15601 ggtaaagccc aggcccgcgg cacgctggtc ggtatcgaaa taaccgccaa tcagttttgg
    15661 taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca gaccgagcac
    15721 ccagacgttt gcgccgccaa tcgcaaatac cggaataatc agcagctgcg aggccagcag
    15781 gctacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagccac ctacgcagca
    15841 tcccaccgcc gcgccaaagc cactaaagaa cagcacattg gctacagtat gcgggttata
    15901 agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg agtagaggaa
    15961 agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttgc ctgtactctg
    16021 caccataaag ctgataaaga ttgcggcgca taacagccca agaacagcga cgatcgcggc
    16081 attttgcagg ttaccggcga agcagaacca cagcgcagta gccgccgcca gtgtcattac
    16141 gatattggca atgcgatgtt caccacggta gagaatatcc accattgtgc gtactggtgc
    16201 tttacctgcg tgtttctctt tccagtcttc cgcttccggg atgtttttac gcagccagag
    16261 agcaaagatg attggcaaaa tgccgataaa gaacagcgca cgccagcccc agaccggaac
    16321 caccaggcta tagacctgag cggcaacgac ggcccccaca gagaagcctg aaatcagaaa
    16381 accactggct ttgttacgca gatgttttgg ccagctttca atgacatagg tggcgctgga
    16441 accgtattca cccgccatcc ccatgccgat gaccagacgc gcgataaaca tggtgatgta
    16501 gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc tggtgaccat
    16561 tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc cgaaccagcg
    16621 agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc
    16681 ctgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc
    16741 caaccaggca gcggaaaatg cgcgccattg tgcacggttg agatggcgat accacgggat
    16801 attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg
    16861 tataaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg gcgagcgagg
    16921 ggaaacaact cacccgcgct cttgcatcaa ctgctgggcc agcgccttca gttctggcag
    16981 atatttttca tctaccggtc caaacggttt gcggcacagc ggcacagaaa cgacatccat
    17041 ataatggagg acagttttca ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac
    17101 tttattgcat tcagtttgca gtttctgcgc ggtctggata tcgccttctt tcagcgcctt
    17161 aacgatcccc tgatagcgcc agcccatgat gttgtaggta ctaccgatac caccatcagc
    17221 gcccgccagc agaccagagg cgaagatttc gtcgtaaccg ttatagagca caagatcagg
    17281 atgttcacga cggatctgct ccatctgata gagatcgcca gaggtctgtt tcagcgcacc
    17341 tacgccaggc aatgtaacaa gtgtgttgat ctgatccagg gtcagtttta ccccactcag
    17401 ggctggaatg ttgtacacca ccatcggcaa accatccgcc gaatcaataa ttgcccgata
    17461 gtgatcgcag tgttcttcaa agctgaaagg atagtagaac ggcgtgacgg cggagacggc
    17521 atcgaagcca taacgtttag ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca
    17581 accgacgtgg gcgatgagtt taatcttacc tttcgcctct tcggcgacga tttccagtac
    17641 ctgttcacgc tcggaaaggc tttgtacaaa ggcctcgccg gtcgaaccac ccacgtataa
    17701 accgtcgatg ccctgctgaa tattgaactg aaccaggcga cgcagactcg ctttatccag
    17761 tgcttgttgt tggtcaaaag gagtcaggag tgcagccatt acgccacgta aattcgttgc
    17821 cataaatacc tctgaagtga tgcttgtctg ataaacgata tacctttata cctgttatac
    17881 cagatcaatt aagcaacacc ccatacagaa agcttataat gcgatctgct tcactaaagt
    17941 ggcattattt ctttttgttg gtggtctgac cgaaagcgtg ccaggtagca gagacgctgt
    18001 tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa
    18061 cgatcgcaat atgctgttga taactaacgt tgttatgttc gtgcagtgcc tgatcggtaa
    18121 ccgttgggcg tgcggcaata agccagtcga gcagggcaac gtggatcgcc atgaagattg
    18181 ggttaccggg gatctccgcc agcacgcggt ggaaatcaac gtctgaacga atgaatgccg
    18241 cgttgttatc cagcgactga ctgttgattt ccagtgcttt tgccagcaaa tcgatttgct
    18301 catcggtggc atgttcagcc gcatagcgca ccaggctgga ttcaaagaac agacgtaatt
    18361 gttcgaaatg ggtaatccca ccaggatgag aaaggaaatc tttcgccatg ccggaaagct
    18421 cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc acgttcgccg ttgttgattt
    18481 gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg cacagaagga cgcccgacgt
    18541 taaagaacgc catcagttcg cgttcagacg gtaattgttc accttcgcca aattcacgac
    18601 ggcggatcat ctgttccagc tcttcttcca ccatttcgga gagtttttta cgcgccagcg
    18661 ggcggctacg caagttgcga ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg
    18721 cgttcataag gcccattctg taaggtcagt gtgattaaca tcatcagtga catcctatca
    18781 caggattgaa agtaggggaa aatggcaggt ttttctcttt gtgcctcatc attaccataa
    18841 ttaacggaat aattaactat tgcgaaaaat taatgtaacg cagataaaaa catcccgttt
    18901 gaattattta taagactatt cacgagcatt atgaatatta tgaatgtgtt cttacaaaat
    18961 aatcataagc gcatattttt taatgaaaaa tcacctcacc tacaattaaa aacacgacat
    19021 ccgcaccata aatagacttg caaaaaatat aacatcattg ttttcaatct gccgtttatg
    19081 ggattgaccg ttttcttttg acacggagtt caacaatgtt cggcataatt atatctgtca
    19141 tcgtattaat tacgatgggc tatttgatcc tgaaaaacta caaacctcag gtggtgctgg
    19201 ctgccgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttcggt ggtgtactcg
    19261 atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc ctgcgcatgc
    19321 tgtccaaccg cattgccgga ttggggctgt cgattatggc ggtgggcggt tatgcccgct
    19381 acatggagcg cacaggagcc agtcgcgcga tggtgagctt gctaagccgc ccgttaaaac
    19441 tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggccaa atcatggcgc
    19501 agtttattac cagcgcctcc ggcctgggta tgttgctgat ggtcacctta tttccgacgc
    19561 tggtgagtct gggagtaagt cgcctctctg cggtggcggt tatcgcaacc acgatgtcca
    19621 ttgagtgggg gattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa
    19681 ttgccactta cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg
    19741 cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg
    19801 aacaggcaga gctaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg
    19861 tgatgccgtt aatcttgatg ctcggctcgc tgttcctcgc ccacatcggg ctgatgcagt
    19921 cagaactgca tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt
    19981 tcttccgcaa gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt ttgacggcat
    20041 gggtacgcag tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg
    20101 cttaacgacg attggcactg ttgatgcggt tatcaggggt gctgagcatt ctggtctggg
    20161 cggtattggc gtgatgatta ttatggcgct agtcattgcc atttgtgcca ttgtgatggg
    20221 ctctggcaat gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg cagccggact
    20281 acatgtacca gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgg
    20341 tttcgccgat tactgcggtg gtggtcgtta cgtcaggaat tgcaggcgtt tcgccttttg
    20401 cggtggtgaa gcgaacagcg atccccatgg cagtcggttt cgtggtgaat atgattgcca
    20461 caatcacgct attttattaa gccattaaaa gacaaaacag gccaccaggg cctgtttttg
    20521 tattacttca caacgcgtaa tgccggtcga ccaccgcgtg gtggctgcgg aggttcatcg
    20581 tcaggatgag tgtcatcatc gtgatctggc ttgtcgccat caataaccga cataacggtt
    20641 tcgttgtctg ccgatgcctc ttcatcattc atgatgctgg tatcttcatc gtaggcagct
    20701 tcaggctcaa acatcgtgcc tgcgccattt tcacgggcgt agatagccag cacggcagcc
    20761 agcggcacag aaacctgacg cggaatgccg ccaaagcgcg cgttaaagcg tacctcatca
    20821 ttcgccagtt ccagattgcc gacagcacgc ggcgcaatgt tgagtacgat ttgcccgtca
    20881 cgcgcatatt ccataggaac ctgcacgcca gggagcgtca catccaccac caagtgcggc
    20941 gtgagctggt tatccagcaa ccactcatag aatgcacgca gcagataggg acgacgtggt
    21001 gttagctgtg acaaatccat acagattaac tccggcccag acgcatttca cgttctgctt
    21061 cagttaaaga agcaaggaaa gagtcacgct caaagacgcg ggtcatatag cctttcagct
    21121 ctttcgcacc cgggccgctg aactcgatgc ccagttgcgg cagacgccac agcagcggag
    21181 caagatagca atcgaccagg ctgaactcat cgctcaggaa gtacggcttc tgaccgaaga
    21241 ccggcgcaat cgccagcagt tcttcgcgca gttgcttacg tgcggcatct gcttcagaag
    21301 ctgaaccgtt gatgatggtg ttcatcagcg tgtaccagtc tttttcgatg cgatgcatgt
    21361 acagacggct ttcaccgcga gctaccgggt aaacaggcat cagtggcgga tgcgggaaac
    21421 gctcatccag atattccata atgatgcgag attcccacag ggtcagctca cgatccacca
    21481 gggtcggaac gctctgattc gggttgaggt caatcagatc ctgaggcgga ttgtcctttt
    21541 ccacgtgttc gatctcgaaa cttacacctt tctcagccag cacaatgcgg acctgatggc
    21601 tatagatgtc agtaggaccg gaaaacagcg tcattaccga acgtttgttg gcagcgacag
    21661 ccatgaaaac ctccaggtat agtcagaatt tttactgcta ccagccacca ggtggccagt
    21721 cagaagttgt gttacccagt aaggaacgac tctctttgtt cgaaaatcaa acaaaaaatg
    21781 agcaataccc gacatttggg cagaaaattg gatgatagtt taccagattt tgcgaccttt
    21841 gtggtgagtc gatgccggaa atggggaaaa agagatgcgc tttagtctga aatagttgac
    21901 ttagtccctt attggcgatg tggtttttgt tttgcctgtc tgtcagacgg tagcaaaaag
    21961 caactttcca atttttacgc tgattcagaa tttaaccata aaaaaacccg ccgaagcggg
    22021 ttttttcgca aattgttttc tgccggagca gaagccaatt aacgtttgga gaactgcgga
    22081 cgacgacgtg ctttacgcag accgactttc ttacgttcaa cctgacgagc gtcacgagta
    22141 acgaagccag ctttacgcag ttcagaacgc agggactcgt cgtattccat cagagcgcgg
    22201 gtgataccgt gacggatcgc accagcctga ccagagatac caccaccttt aacggtgatg
    22261 tacaggtcca gtttctcaac catgtcgacc agttccagcg gctgacgaac taccatgcga
    22321 gcagtttcac gaccgaagta ctgttccaga gaacgttggt tgattacgat tttaccgttg
    22381 cccggtttga tgaaaacgcg agctgcggaa cttttgcggc gaccagtgcc gtagtattga
    22441 ttttcagcca ttgcctataa tcccgattag atgtcaagaa cttgcggttg ctgtgccgcg
    22501 tggttgtgct cgttacccgc gtaaactttc agtttacgga acatagcacg acccagcggg
    22561 ccttttggca acatgccttt aaccgcgatt tcaatcacac gctcaggacg gcgagcaatc
    22621 atctcttcaa aggtcgcttg tttgatacca ccgatgtggc cggtgtggtg atagtacact
    22681 ttgtcagtac gcttgttgcc ggttacagca actttgtcag cgttcagaac gatgatgtaa
    22741 tcaccggtat ctacgtgcgg agtgtattcc gctttgtgct taccgcgcag gcgacgagcc
    22801 agttcagtag ccagacggcc cagagtttta ccggtcgcgt caacaacata ccagtcgcgt
    22861 tttacggttt ctggtttagc tgtaaaagtt ttcattaaaa gcttacccaa taaatagtta
    22921 cacgttggtg aacacccaaa cgtcttcaat tgttgaggtt cacacgacaa agtccggcaa
    22981 acctacccct tcgaatagcc tatgccagca cacaaaaagt tttgggaaaa aaactttctt
    23041 gtaacgtggg gtcgcaggat tatagagaag tcggggtcaa agatcgaccc ctttttgtga
    23101 tttgtgacag gttttaaccc gccaaatgct cgcgcttcag atactcttcg ctttgcatct
    23161 cttgcagacg tgacaggcaa cgctggaact caaacttcag ccgatcgccc tgataaattt
    23221 catacagcgg cacttctgca ctcaccacta atttgacatg gcgctcgtaa aactcatcca
    23281 ccagcgcaat aaagcgccgc gcttcgctct ccatcaaccg cgtcataact ggtacatcaa
    23341 acaacatgac cgtatgaaag agacgtgaga gcgcaatata gtcatgctga ctgcgggcgt
    23401 cgacgcacag cgtagtaaaa gagaccgcca gcgtctggtt ctcgacgccc attgtcgcca
    23461 atggccgatg gttgatttct aacgtcggtg aattttctcg tttcgccccc gccagcgcca
    23521 accatagttt atccatctgc gcccggcttt catcgttgag tggcgaaagc cacagatgcg
    23581 cctgagtgag tgtacgcagt cgataatcaa caccagcgtc cacgttcatt acatcacaat
    23641 gctgtttaat ggcatcgatt gcaggcagaa aacgcgcacg ttgcaggcca tttcgataaa
    23701 gttcatccgg cggaatattt gacgtcgcta ccagggtaat gccgcgggcg aacagggctt
    23761 tcatcagacc gccaagtagc atggcgtcgg taatatcaga aacaaaaaat tcgtcaaaac
    23821 agagcacgtc agtttcggct ttaaagcgat cggcaataat ttccagcgga tcggtctgcc
    23881 cctgtaaggc agttagctct tcgtgcaccc gcagcataaa acggtgaaag tgcaggcgct
    23941 gtttccgctc tcccggcagg ctttgataga aaaggtccat cagccaggtt ttcccgcgtc
    24001 ctacaccgcc ccacatatat aagccacgca ctggcgtatg ctttgtgtct tcgcgtttac
    24061 cccacagctt accgacccgc gccattagcc cactcgtcct gggggctggt ggcctgctat
    24121 tgatgagttc ttgataaata atttccaggc ggctgacggc ctctttttga acgtcgtcgg
    24181 gttgatggct gccttcattg agcgccttca ggtattgcga tgttggggta acgctttgca
    24241 tgatcttatt gttattcctt gaataatcgg tgcgccgttg ttcacggttg acgaaaaaaa
    24301 ggccgttcta cactacgcga tatgcagtcg ggattccact tctgtggaat taacggttat
    24361 agtggcataa tcagccgcag gcatggagcc tgaagccaac accctacgga aacaaaagac
    24421 aacgggagat gttcatgacc tgggaatatg cgctaattgg gttagtcgtc ggcatcatta
    24481 ttggtgctgt ggccatgcgt tttggtaatc gtaaactacg ccagcaacag gcgttgcagt
    24541 acgaactgga aaagaataaa gctgaactgg acgagtatcg cgaagagctg gttagccact
    24601 ttgcccgcag cgcggaatta ctggatacca tggcgcacga ctatcgccag ctgtatcagc
    24661 acatggcgaa aagctccagc agcctgctgc cggaactgtc tgctgaagca aacccgttcc
    24721 gtaatcgtct ggcagagtct gaagccagca acgatcaggc accggtgcag atgcctcgcg
    24781 actattctga aggtgcatcc ggcctgctgc gtactggcgc gaagcgcgac taatttattt
    24841 ttcgggcgca gccattgcgc cctcctcttc tctccctccc cgactatcat ttaatctggt
    24901 gtctcattgt tagccgtctg aaaattcaat aacatcaaac tgttttgaat ctcttttctt
    24961 atcattcagg tacgagagca ggaataatga aaaaacaaac ccagctgttg agtgcattag
    25021 cgttaagtgt cgggttaact ctctcggcgt catttcaggc cgtcgcgtcg attccaggcc
    25081 aggttgccga tcaggcccct ctccccagtc tggctccaat gctggaaaaa gtgcttccgg
    25141 cagtggtgag cgtacgggtg gaaggaacgg ccagtcaggg acagaaaatc ccggaagaat
    25201 tcaaaaagtt ttttggtgat gatttaccgg atcaacctgc acaacccttc gaaggtttag
    25261 gttccggtgt catcatcaac gccagtaaag gctatgtgct gaccaacaac catgtgatta
    25321 atcaggcaca gaaaatcagt attcagctca atgatgggcg cgagtttgat gcaaaactga
    25381 ttggtagcga tgaccagagc gatatcgccc tgttacaaat tcaaaacccg agcaaattaa
    25441 cgcaaatcgc tattgccgac tccgataaat tgcgcgtcgg tgattttgcc gtagcggtcg
    25501 gtaacccatt tggccttggg caaaccgcca cctctggcat tgtttccgca ttaggccgca
    25561 gcgggttgaa tcttgaaggt ctggaaaact ttatccagac agatgcttcc attaaccgcg
    25621 gtaactccgg cggtgcacta ttaaacctta acggtgagtt aattggcatc aacactgcaa
    25681 tccttgcgcc tggcggcggg agcgtcggga ttggatttgc catccccagt aatatggcgc
    25741 gaacactggc gcagcagctt atcgactttg gtgaaatcaa acgcggtttg ttaggcatca
    25801 aaggcaccga gatgagtgcc gatatcgcca aagccttcaa ccttgacgtg cagcgtggcg
    25861 cgtttgtcag cgaagtgttg ccaggttctg gctcggcaaa agcgggcgtc aaagcgggcg
    25921 atattattac cagcctcaac ggcaaaccgc tgaatagctt tgctgagttg cgctctcgta
    25981 tcgcgaccac cgagccgggc acgaaagtga agcttggcct gctgcgtaac ggcaaaccac
    26041 tggaagtaga agtgacgctc gataccagca cctcttcgtc ggccagcgct gaaatgatca
    26101 cgccagcgct ggaaggtgca acgttgagcg atggtcagct aaaagatggc ggcaaaggta
    26161 ttaaaatcga tgaagttgtc aaaggaagcc cagctgctca ggctggcttg caaaaagacg
    26221 atgtgatcat tggcgtcaac cgcgatcggg tgaactcgat tgctgaaatg cgtaaagtgc
    26281 tggcggcaaa accggccatc atcgccctgc aaattgtacg cggcaatgaa agcatctatc
    26341 tgctgatgcg ttaatgtcgt aaaccgggca tcaggcttac gtgtgatgtc cggttaactc
    26401 gtggtatgct gctgccgttc ccttttttaa tgacgcctcc atcatgtttg tgaagctctt
    26461 acgttccgtt gcgattggat taattgtcgg cgctattctg ctggttgcca tgccttcgct
    26521 gcgcagcctt aacccgcttt ccactccgca atttgacagt accgatgaga cgcctgccag
    26581 ctataatctg gcggttcgcc gcgccgcgcc agcggtggtt aacgtttaca accgtggttt
    26641 gaacaccaac tctcacaacc agcttgagat ccgcaccttg ggatccggtg taatcatgga
    26701 tcaacgcggt tatatcatca ccaataaaca cgtcatcaac gacgccgatc agatcatcgt
    26761 cgccttacag gatggtcgtg tatttgaagc attgctggtg ggatctgact ctctaaccga
    26821 tctggcggta cttaaaatta atgccactgg cggtttacct accattccaa ttaatgcacg
    26881 tcgcgtaccg cacattggcg acgtagtact ggcgatcggt aacccgtaca acctcgggca
    26941 gaccattacc caggggatta ttagtgccac gggtcgaatc ggtctgaacc cgaccgggcg
    27001 gcaaaacttc ctacaaaccg atgcttccat taaccacggt aactctggcg gcgcgctggt
    27061 gaactcgctg ggagaactga tgggcattaa cacgctgtcg tttgataaga gtaacgatgg
    27121 cgaaacgccg gaaggtatcg gctttgcgat tcctttccag ttagcaacca aaattatgga
    27181 taagctgatc cgcgatggtc gcgtgatccg cggctacatt ggtatcggcg gacgcgagat
    27241 cgcaccactg cacgcgcaga gcggtggtat agatcaactg caagggatcg tggttaatga
    27301 agtgtcacca gacggcccag cggcgaatgc gggtattcag gtcaacgatc tgattatttc
    27361 ggtggataac aaaccggcca tctctgctct ggagacgatg gatcaggtgg cggaaattcg
    27421 ccctggttcg gtgatcccgg ttgtagtgat gcgtgatgat aagcagttaa cgctgcaggt
    27481 caccattcag gaatatccgg caaccaatta agtcgagtgc tcaaaacaaa aaccggagtc
    27541 tgtgctccgg ttttttatta ttcgctaatc aattacttat taacgaactc ttcgcccagg
    27601 gcgatatctt tcttgagcgt atccagcata ccttccagcg cgttctgttc aaatgcgctc
    27661 agggtaccga tagatttacg ctcttccacg ccgtttttac ccagcagcag cggttgagag
    27721 aagaaacggg cgtactgacc gtcgccttca acgtaggcac attcgacaac gccttgttcg
    27781 ccctgcagtg cacgaaccag agacagacca aaacgtgcag ctgcctggcc catagacagg
    27841 gttgcagacc cgccaccggc tttcgcttca accacctcag tacccgcgtt ctggatacgt
    27901 ttggtcagat cagccacttc ctgctcggta aaactaacgc caggaacctg tgacagcagc
    27961 ggcagaatgg taacaccaga gtgaccgcca ataaccggca cttcaacttc gcctggctgt
    28021 ttgcctttca gttccgcaac aaaggtgttg gaacgaatga tatccagcgt ggtaacgccg
    28081 aacagtttgt ttttgtcata aacaccggct tttttcagca cttcagcagc aatcgcaact
    28141 gtggtgttaa ccgggttagt gataatacca atgcacgctt tcgggcaggt tttcgcaact
    28201 tgctgtacca ggtttttcac gatgccggcg ttaacgttaa acaggtcgga acgatccata
    28261 cccggtttac gcgctacacc tgcagagata agaacgacat ctgcgccttc cagcgccgga
    28321 gtcgcatctt caccagaaaa acctttgatt ttcacagcag tagggatatg gctcagatcg
    28381 acagccacac cgggagtcac tggagcgata tcatacagag agagttctga acctgaaggc
    28441 agttgggttt ttaacagtag tgcaagcgcc tggccaatac cgccagcagc gccgaggact
    28501 gcgactttca tcctaaactc cttattatat tgataaacta agatatgttg ctccgctgcc
    28561 gcgaccttaa tccacaaaat tgccgtttac aatgaccaca tctcaagaat gtgtagtcac
    28621 gcaagtttag cgtttatgca tttaattgcc gtaatcagga acctaactta cgtaattaac
    28681 agtcgcttat gcattagcgc aacattccaa caggtggtga caatataccc taccgttcag
    28741 ccaaaacaac atcaatttga taacaattaa tttactttta agcagaattt gcatgccgtg
    28801 acgcaggcat gtttctcaat aacgaaattt gataaaatcc cgctctttca taacattatt
    28861 tcatccttct tcagggctaa ctgtttgcat aaaaattcat ctgtatgcac aataatgttg
    28921 tatcaaccac catatcgggt gacttatgcg aagctcggct aagcaagaag aactagttaa
    28981 agcatttaaa gcattactta aagaagagaa atttagctcc cagggcgaaa tcgtcgccgc
    29041 gttgcaggag caaggctttg acaatattaa tcagtctaaa gtctcgcgga tgttgaccaa
    29101 gtttggtgct gtacgtacac gcaatgccaa aatggaaatg gtttactgcc tgccagctga
    29161 actgggtgta ccaactacct ccagtccatt gaagaatctg gtgctggata tcgactacaa
    29221 cgatgcagtt gtcgtgattc ataccagccc tggcgcggcg cagttaattg ctcgcctgct
    29281 ggactcactg ggcaaagcag aaggtattct gggcaccatc gctggcgatg acaccatctt
    29341 taccacccct gctaacggtt tcacagtcaa agacctgtac gaagcgattt tagagctgtt
    29401 cgaccaggag ctttaatctc tgccccgtcg tttctgacgg cggggaaaat gttgcttatc
    29461 cctctcaatc ccctgctttc ccctgcgatt catttaacga atagtgcgtt ttaccgcgac
    29521 atatcattca cgcaatgaat acatgcggta aaaaaagcac attatgcaaa attcattatc
    29581 tgattgaaaa gactggtatt tataataaat aaccgtgttt ttaatacttt tttgttatta
    29641 aaattcacat ttttaacact tagtatcaac tgaaacagtt agcgtgatat taattagttc
    29701 aataattagt gtatacttga ttttgtgata tgggtcacga aacaaaggcc cagctaaaag
    29761 attatgtcga ggtaaaaatc atgaaaatca aaaccactgt agctgcatta agcgtacttt
    29821 ctgttctctc tttcggtgca ttcgctgccg actccattga tgctgcacaa gcacaaaatc
    29881 gtgaagcaat cgggaccgta tccgtaagtg gtgtggcgtc ttcgccaatg gatatgcgtg
    29941 aaatgctgaa caaaaaagcg gaagagaaag gcgcaacggc ctaccagatt actgaagctc
    30001 gtagcggtga cacctggcac gctacggctg aactgtacaa ataaaccctc atcgtcttgt
    30061 ccgacgatat tgcccccggt tcgggggctt tttttgcgct aatgacgaac attaaaacgc
    30121 aaatgccctt ccagctcttc ctctgcctca tcaaacagca atattaacgc gccaaaacgg
    30181 cgacgcgttt tctctcccag atgaacaaat tcaatctcaa gtggtagcgg caggacatcg
    30241 ttcattaaca catcccatag tgagtcgaga tcgcgtacct tatctttcgc cagaccaaag
    30301 gtttggctaa agtcacgata aaaatcctcc tgactctcaa tctcatcaaa atcaaaggta
    30361 taaatattca tctgttgcca ccgtcacgtt tcgcggcgag ttctgccgtg catcgctaag
    30421 tatagccatg aaaaaaccga cgcttttggc gtcggttttg acttaactat cggtcaacgc
    30481 atgttgatac ttatggagca tccccgccag ccgatgaacc ggttccgtca cctgcggtgg
    30541 cgcttgccag atgcgtagct tttcctggta gatttccagt tcttccagca actggccaaa
    30601 gtaccgacga cgtttatcat cgctacgggc agatatcaca tggtctgctg tgcggcgcat
    30661 ttgtcggtga aacgccgata aatcctcgtt aaccgggatc ggcgcatcac gcaggcgctg
    30721 gtgcgcgata atcatcgtca gcgccaggcg aaatttcggc aaatcccctg ggaacttatt
    30781 catcagcaaa aacagctgct gataaagtgc cgggaggtgg ttctctttac gacgtgccac
    30841 attggtagtc atcgcggaaa cagcggcaga aacaaactga ttaagcagta cacgtccagt
    30901 cctgtcgcgc gatttatccc gcaccagcaa aataacggtg aacgcgagca cacagccgac
    30961 gatttgccct aatgcgctgt cgagaaactg actgaaatgg aaagtcatcg ggttatccag
    31021 cacgataata tttatggtgc tggccagcgc ccccatcgag cccagtcgcc gtttctgtac
    31081 ttctattccg aggaagaatc ccagcactgc caggctaata cacagcagca acatgctctg
    31141 ttgggtatta gggataatca ccaaaaagta gagcagccct aacggcagcg cggccagcgt
    31201 cccgtagata aagtcgatcg ccaccatgcg tggattcggc aaacgcattg ccagtgacgt
    31261 cacgaccgca atcatcacca ttgcaccact gccggaagtc cagcccgtcc acagccagaa
    31321 aagcgttccc agaatgcagg aaagtgtggt tcgccagaag ttaaccatcg catgatgacg
    31381 ttcggctgac tctactttga cttccggttc gccttgcagg atctcttctt cggtggcgtt
    31441 gatttttgtg ttactgataa cgccgcgctt gagaagctga taacgcgttg ccgccgcgac
    31501 ccagctataa atggtgacag gcgtttcccg ttccccggtc caggcgataa ctctccgcag
    31561 gcgtttgagc tgcttgtgga cgtcctgcgc ggtttctacc ggcgtgtcaa aaaattcgcg
    31621 gaaagtatca gtgatcaatt ccgggcgcgt attctgaata agataagttt cgcaggattg
    31681 ggtaatcagc gtcagcgata gcgtattgat cgcttttaaa cgtcgattgg cccgcgccca
    31741 gcgggaagat tccatattca ggttgctgcg catgccttgt agcgccgtgg tgcgtcgcac
    31801 caggtcgccc caggctttat cgacaacttc accatcgcca tgcttgatac agagttgcat
    31861 taattgatat tgcgcgacca gcaaactttc cagctctcga tccacttctt gtttgatcga
    31921 tcgcggagaa aagagcaaat ccgccataat cgcacacaca ataccgataa cgatctcgct
    31981 acaacgttcg acggcaaact gcggcgtaag caatggttcc ggctgaatgg tgatcacaat
    32041 gatcagcgcg gtataaccgg ccagccccca cgcgtacgag ttttctattc gtaccagcga
    32101 ggatatccag gtacaaaaac cggcccagat acagcacacc agaatcatca ataatggtgc
    32161 gcggatcatc gcaatgatga tcaccagtcc ggcaatacag ccaataaatg tgccgatgat
    32221 gcgcaaaaag ccacgatagc gaatagcgcc agaatacggt tcacctcccg cagcaaaggc
    32281 cggaccggcg gcaacaatcg ccgctgtcag taccgcccag cgtggcgttt ccagctggaa
    32341 gtgaaagcca acaaacagcg ccagtacaat ggcggtcgcc agttttaccg caaagcgaat
    32401 atgttggtta gcaatggaga aaatacccat cgtgattaac caaactcacg caggcgatgg
    32461 gccattttac ggaagaacga atcctggctt tcgtcgcgat cttgtttgcc agtgaccacc
    32521 actgtagcag tggtgcccgc aggccagatg ttctcttgct ggttgtcgag acgaatacga
    32581 accggaacac gttgcgccaa acgcacccat tcaaggttag agtcgatggt cgccatccct
    32641 ttgtcgtcac gcgtgctgct ggcgttggtg accccagcgg caacactatc aacagtccct
    32701 ttcagcactt tgttactgcc aagcggcgtg atctctgcac gataccccgg acgcacccct
    32761 tccagcttag tttcttccat ataggccagt acatagaagg agttctgttt caccagcgca
    32821 accgccgttg atcctcgagt aataaactca ccggtataga cgttgaggtt ggtcacccag
    32881 ccatctgctg gcgcgcggat caccgtgcgt tcaagatcca gttttgccag atcgcgggtc
    32941 gcctgcgctt tcgctaactg atgcagaacc gtttgtagta cgttgttagc ctggtcgatc
    33001 tcttcgcgag acatcgcctg cacaccgaga cggttacgac gtccggcctc ctggcgtttc
    33061 tcctgtgcca gtacctgata ataagcaaca tcggcttgcg cttcctcaag cgccttttga
    33121 tagcgcggct ggtcgatggt gaacagtatc tgtccttttt tcaccagctg gttatcatga
    33181 acattcacct gggtaatgag tccagaaacg tccggcgcga tcgcaacgac gtcagcgcta
    33241 aagcgcgcgt cacgcgtcca gggggattcg gtgtaataga cccaggcatt aaaaattgcg
    33301 atgaaggcca gaatgactaa tacgaccgtg atggccgtac gggagaattt tcttattagt
    33361 gttttcactt caacctcaaa cgaacagtcg cgatatcaaa taaaacaagc agcaatagag
    33421 cgcggtgttg aacaacgccg gatgccagac aaagtcgtag atacctgttg gcacaagtac
    33481 ccggcgcacc agccagaaaa tcgccagtga taaaagcaat tcaaaaaata tcggtgggaa
    33541 ggacagccca aacaccacga taacgggaaa cagactcatg ttgaccttgg ttgtatagag
    33601 agagcaggcg ttattatttt cagcatctgt cgccgcagag aagggcatgg aaagccgggc
    33661 gagagcaaca ttgctgtaga ttgatattta atatattagc gtaactgtta tgctgttatc
    33721 tatattatgt gatctaaatc acttttaagt cagagtgaat aatggaacga ctaaaacgca
    33781 tgtcggtgtt tgccaaagta gttgaatttg gctcttttac cgccgccgcc agacagctac
    33841 agatgagcgt ttcgtccatc agtcagacgg tgtcaaaact ggaagatgag ttgcaggtaa
    33901 agctgttaaa ccgtagcaca cgcagcattg gcctgaccga agccggtaga atttactacc
    33961 agggctgccg tcgtatgctt catgaagtgc aggatgttca tgagcaactg tatgccttca
    34021 ataacacccc catcgggacg ctacgcattg gctgttcttc aactatggca caaaatgttc
    34081 tcgccgggct gacagccaaa atgctgaaag aatacccagg tttgagcgtc aatctggtta
    34141 ccggaattcc agcccccgac ctgattgccg acggtctgga tgtggtgatc cgcgtcggcg
    34201 cgttgcagga ttccagcctg ttttcccgcc gtcttggcgc gatgccaatg gtggtgtgcg
    34261 ccgcgaaaag ctatctcaca caatacggca taccggaaaa acccgccgat ttaagtagcc
    34321 attcatggct tgaatacagc gtgcggcccg acaatgaatt tgaactgatt gcaccggaag
    34381 ggatctcgac tcgcctgatc ccgcaaggga gatttgtgac taatgatccg atgacgctgg
    34441 tgcgctggct aacggcgggt gccgggatcg cctacgtgcc gctgatgtgg gtgatcaacg
    34501 agatcaatcg cggtgagctg gagatcctgc tgccgcgtta tcagtcagat ccacgcccgg
    34561 tttatgcgtt atataccgaa aaagataagc tgccgctgaa ggtacaggtc gtgatcaact
    34621 cgctgacgga ttattttgtt gaggtcggta aattgtttca ggagatgcac gggcgcggga
    34681 aagagaagta attcatattg tactgttacg ttctacaaac ctgtgccaac gggttcccct
    34741 caccctaacc ccagaggagc gaggggaccg ttcgtgcacg tagaaagatt aattatcctt
    34801 ctgaaaataa tgaaattatt acgcagtacc gccaacagtc aggttatcaa ctttcagcgt
    34861 tggctggccc acgccaaccg gcaaactttg cccttcttta ccgcagacac ccacgccgtt
    34921 atccagtttc aggtcgttgc caaccatcga aatctgctgc atggtttcga taccggaacc
    34981 aatcaacgtc gcgcctttca ccggcttcgt tactttaccg ttttcaatca gatacgcttc
    35041 tgaagtggag aaaacgaatt tgccggatgt aatatccacc tgaccgccac caaagttcgg
    35101 tgcatagata ccgtactcaa cggattcaat aatttcctgc ggggtcgatt tacccggcag
    35161 catataggtg ttggtcatac gcggcatggg cagatgggcg taggattcac ggcgaccgtt
    35221 gccagtcggc gtcatcccca tcaaacgcgc gttgagttta tcctgcatgt agcctttcag
    35281 aatgccgttc tcaatcagca cgttgtactg gcctggcgta ccttcgtcat caatcgccac
    35341 cgaaccacgg cgatcgacca tcgtgccgtc atcaaccacg gtacacagtt ctgaagccac
    35401 cagctccccg acctgcccac taaatactga agtgccgcgg cggttgaagt cgccttccag
    35461 accgtgacct accgcttcat gcaacagcac gcccggccaa cctgcgccaa gtactaccgg
    35521 catggtgccc gctggtgcag caacggcaga aagattgacc agcgccatac gcactgcttc
    35581 ttttgcccat gcatccgcac ggacttcgcc gtcaagatcg gcaaggaaga actcataacc
    35641 aaaacgaccg ccgccgccac tggcaccgcg ttcgcgtttg ccatcttctt cgaccagaac
    35701 gctcacggaa agacgcacca gcggacgaac atccgccgcc agcgtgccgt cggtggccgc
    35761 aaccagaatt aattcataaa cgccgctgag gctggcagtc acttcttgta cgcgcttgtc
    35821 cgcttcgcgg gcaaccttat cgacgcgacg caggatatcc agcttctctt cacggctcat
    35881 gctttgcagc ggatctaccg aggtatacaa cgggctatgc tctaccgcgc ccagcgtctg
    35941 tactttgcca tcaccactat cacggacgat ggtgcgcgcc gcttgcgcac tctgttccag
    36001 cgccagcagg ctgatttggt cagcgtaagc aaatccggtt ttttcaccac tgattgcacg
    36061 cacaccaacg ccctgatcga tgttgtaaga accatcttta ataatgcggt cttctaaaac
    36121 ccaggattcg tgatagctcg actgaaaata gagatcgcca taatcaaggc gacgttcggc
    36181 cagttgaccg aggatcgcga acaagtcctg atgtttcagg ccgttcgccg ctagcaattg
    36241 ttcacttacc aggttcagac tcatcgtttt tgctactcgt tagttactgc agtagaggat
    36301 tttttacggc tgccggatgc ggcgtgaacg ccctatccgg cctacagtta tgttccggct
    36361 cgttggcctt tggcaacgat tatcatatga gattggggca attacgcgcc ctcgtcaaat
    36421 cattgcgctt tttctttacg cggttggcgc aacacttcgt tgatttgcgg atcgtccagc
    36481 ggacccgaaa tgtgatagcg caaaatggag actttgctcc acagcggccc cagcacttta
    36541 ctggctgcaa atactgccgc gccaacaatg gggttaaccg caaaagccgc agccacgccc
    36601 accgtcgcag aaatctctgg tgcgacaacc gcttccatat tcaggtcgcg acgtaccaga
    36661 tttaccgacc ctttcatggc gatatccgcc tccaggccat ccaccagcgt gtcgtcggtg
    36721 tgcataaccc cgtctttaat ccacgcggtg ctgcgaatgg agtcaaaata gaacccttcg
    36781 ccaaaagtgt ctctgaaatc aaaacgcagc ttacgcatca gggcatcaac gctcaataag
    36841 cgcagcaatt gcccggcatg tccggtattg atttcggtaa tttcgccttt acccagttga
    36901 gtatgaatga tgccattcaa cgtcgcctca tctggttgcc agggtgcttt gcgccagtgt
    36961 aaatcgtaat ccacattaaa tgacgactgg cgtatgggcg tcgtgacacc aaaaaattct
    37021 gcggcggcat caattttctg cccgcgcagt tttcctttca gcgaggtacg ttcattctcc
    37081 ggattattaa cccattcacc atcggcagta agccgcgaga aaccagtatc aatcagtcca
    37141 ttggtcagcg ttaacgtatc gccagaaatg gtgatatcac tgtcaatgcg accgaatttt
    37201 tgcccccaga accagcactc tgtgcatcgt atttgtgcgt ccggccagcc acggaagtta
    37261 atgcgctccg ttgtcgggaa aggtgatgac ggcgttgaat caccacgagt tttcgccacg
    37321 ctcgggttgt aataaagata tttgatattc gccagccacg gcgcgttatt acgcatcgct
    37381 agcgtggcgt tgatttcacg cccttgcgcc tcaaccaggg tgccatttgc cgtcggttgc
    37441 gaaacaatac tcaggttatt ccattgctga tttcccagtg acaacatagg cgtacgtaac
    37501 gttatgtgtt gtgggaaact cgctgcacca ccgacactct ccgcagcgcc tttctggaac
    37561 agggccagcc actcggcacc attcatcggc ggcatattga gttcaacgcc actttgttcc
    37621 ggcaacggcg ggagcgtttt actgtctgcc gcccaaatag cacgatcgag cgtcagcttt
    37681 tgaccaagca accagcggct attgaaatgg ttatccgcac cagcctgtcc ggttaattca
    37741 aagctgttga gattgccatc aaccttaacg tttaccgcta gtggttcacc cgcaggtttg
    37801 gctaacggtg aaggtaagtg actactcaca ttattcagat cgccgttcat ctctacgtta
    37861 taggtcgcgc cagcatgata aggcagatca atgcccactt taccatccca cgccacgctg
    37921 ccactcaatg cttcgttcac cgcttcaggc agaacgccgg ttttcgccgg ttgccagtta
    37981 ccgttgaggt ttaccgctac ctggtaggct tttgcccctt ctttggtgga aaaatccacg
    38041 ttcaacggct gattaaacca gcttgctgtc agtggttcac tttgcagatc gccattgata
    38101 aagctgaatt taccgctcaa atttttcagg gtgctgtcga gtggtttgat aaacagactg
    38161 ttattacgca gcgtcacttc acctttcgcg gttaccaact cgccgttcag cgggatatca
    38221 agatgtaagc gagcattcac atcgccgtcg agctggagtt cttgcagggt cgcacccagg
    38281 gaatctttca gcggtgtctc atcaaagtaa gggcctacgg ctttacccgg acctttaatg
    38341 tcagcgtcaa tcagcagttt ttcttttgag tagtcaggga tcactgcggt gagattactc
    38401 gcgcgcacgc cgcccagatt aacgccatcg gttttcatcc ataaaccgtc gttaataaag
    38461 tccagttcaa tatcaaggtt agttaatgca ggccagtccg gctggaaggc aaacttcgcg
    38521 ttgcgcagcg gcaccagcac ttcaaactga ccttcgttgt gtttataggg gaagagttgc
    38581 ggattgccac cataaaccag cgtcgcgtta tccgcttcac cgccctgaat cgcgccactt
    38641 aagtaatcaa ccaggtcttt acccatcaag ttttccggga aatagcgcca ggcttgtgaa
    38701 ccatcatcgg tactgatgcc agccagaata cccagccagg gttcatcgtt agcaggttgc
    38761 aggtaacgaa aaccgccgcg cgcatggacg gctttggctt taacgtcaat attacgccca
    38821 tccagctgga aacctttgtc attgttcagc cagcttatag ttgcctggcc gtcggcgatt
    38881 tccagtggcg cacggaatac cgtttcgtaa ggcatctttg cctgcttcat cgacgcggta
    38941 agcaaaccat tttcaacgct gccggaaagc gtcccggaga agtgttccgc acccggtaat
    39001 aatttccatt gcttccaggc cagatcgctc cacgatgcct gaaaacgggt attgtctgcc
    39061 gcctgaagcg ggatatccag cgccagagtg ttaatcttgc cgctcggttg tgtggagcgc
    39121 caaacatcac ccagtgcagg tgaaagtttc gcggccagcg ggcgtatgcc ctccaggcct
    39181 gccagctcca gattactggc gcgaatccgg agttcgtcac tgcgtttatt gtctttgccg
    39241 ccaacgtcct gttccggtat ccaggccagc gtcaatgctc cgctcggcca gggtttaccg
    39301 tccatcgtga tccgtgtatc gggaatagag aactgccagc ccggattttc acgcgtaata
    39361 tgcgcggtca gattatccac cgacagcgta tgcgtttcct tctcgcccaa ccagctggca
    39421 ccgccctgtt tcagccagac gtcaccgccg gttacatcgc ctttgtcgat cgtcatccag
    39481 ccttcaaggg agaactgtgc cgtttccaga gcaatattgt cctgcatcca tttaccgagc
    39541 cacggcttca ggtcgatgtc atccgcctgt agccagacgc gaccattgct taacaacccc
    39601 tcatcatcgc gcaaatccat gcgcacctgc atcacgccgt gctgtccggt aaggctggag
    39661 aggcttacca ggccttccgc acggtgtcga cgtggatcgt tcagccaggt gagttgtggg
    39721 atcgccagct cggcgcgctg accggatggc gtcagaaaac tgacttcact gtcgcggaga
    39781 tcgaaatggt caaattgacg aagaaacaga tcgctgatgt gactggcttc cagactgtca
    39841 tcgctaccac cgctggtgat aggagtgttg gtgcgaaagc gcagctgcca gaaagtgagg
    39901 tcgcgaaact gccagcgcat atgtaacagg ctctgccaga catccagcgc cagagtaacg
    39961 cgtttaaccg aaaattcgcc gccatctttt agttctgcac ggatgtcgtg tgcttcaagc
    40021 gtcgggccaa aattctgcca gctggctgag agctgactgg cttctaccgg catgccagtc
    40081 gcggattcta ttttgttgag gatttccgga cgccaggcgt caagatgcgg taaagcaata
    40141 cgcaggccgc taaccagcag ggctgcgatc acaacgagcg cggctccagt aagcagtaaa
    40201 atccccggca atcgcctcac ccgtcactcc ttgtctgcta aaaatgtgac tcaaaaaccc
    40261 tttgccggat ggcggcccag catctgttta catcattacg acgtcaaact gctcctggtt
    40321 atagagcggt tcaatttgta ctttaacctg tttaccaacg aaaatttcca cttccgccag
    40381 cgagtgtgac tcttcgcctt tcaaggcttc agctactgcc ggagaagcat agaccaggaa
    40441 acggtcggag tcgtaagcat ggtggacacg aacaatctcg cgcatgattt catagcatac
    40501 cgtttccacg gttttcaccg ttccgcgacc gtggcaggtt gggcattcgt tacacagtac
    40561 gtgctcaatg ctttcgcggg tgcgtttacg cgtcatctcc accagcccca gcgccgaaaa
    40621 accattaacg ctggttttca cccggtcttt gctcaacgcc tgctccagcg agtgcagcac
    40681 gcggcggcgg tgatcttcat tattcatatc gatgaaatca ataatgataa tcccacccag
    40741 attacgcaac cgtaactggc gagcgatagc ctgcgtcgct tcaatattgg tattgaaaat
    40801 ggtgtcgtcc agattgcgat gaccgacaaa cgctccggta ttgatgtcca cggtggtcat
    40861 cgcttcggtc tggtcgataa tcagataacc accggatttc agttctactt tgcgttccag
    40921 cgctcgctgg atttcgtttt cgacatcaaa gagatcgaaa atcggctggc gtccggtgta
    40981 atgctccagc ttgctggtca tctcgggaat gtactccgag gtaaactcca gcaatgcttc
    41041 gtaagtcagg cgtgagtcaa cgcgaatgcg gtccagttcg gcgtcggcga aatcacgcag
    41101 aacacgctgc gccagcgcca gttcgccgta cagctgataa cgggtctgcg ggcgtttttt
    41161 acgctccata actttggtcc agacgcgttt cagataagcg gcatcggagg ccagttcagc
    41221 ctcgccaacc ccttccgctg cggtacggat gataaacccg ccctgctcgt cgcaatactc
    41281 tgcgaccact tttttcaggc gttcacgttc tgattcgctt tcaatacgtt gggaaacacc
    41341 aacgtgagaa gcccctggca taaagaccag atagcgagaa ggcagcgtga tatcggtggt
    41401 caggcgcgcc cccttagtgc caagcggatc tttcaccacc tgcaccatca gatcttgccc
    41461 ctgacgaacc agttccgaga tgtcgcgcac cgtgaattgc ttttgttctt cacccgccac
    41521 acattcggtg tgcggcatga tatcggatgc atgaagaaac gcggctttat ccagcccaat
    41581 atctacaaaa gccgcctgca tacccggaag tacacgactt acacgaccct tgtagatatt
    41641 gcctactatt ccgcgtcgcg cctcacgttc aatatgaatt tcctgcagaa taccgccatc
    41701 aatatacgcc actcgcgttt ccgaaggcgt tacgtttact aacaattcag ccgtcatgtt
    41761 tatccctttt ctcacgcagt gcgttaaaat tacttaataa ttcatacgtt tcaaccagcg
    41821 gtaagccgac tacggcgtga tagctgccat ttatcttcct gacaaaacag ccacccagcc
    41881 cctgaatacc gtatgcacct gctttatcta acggttcacc gctggcgaca tagcccgcga
    41941 tgtcttcgtc tgttaacgtt ctgaaagtca catcggtgac caccaggcaa tcgagaatgt
    42001 gctggctgtc ggccaacgcc actgccgtca tcacctgatg ggtctgaccc gataatttgc
    42061 gcaacatctg cgccgcatgc tctgcgtcgc gcggtttctc cagcacttct ccattcagga
    42121 taacgatagt atccgcaccc agcaccggga gatcctgcgc cgtttgcgcg acacccgcct
    42181 gtgctttctc gcgcgccaga cgcacaacat actgctgcgc gctctcctgc ggctgacgct
    42241 gctcctcaat gcccgtaaca atacgttcaa aggtcacgcc aagttgcgca agtaactcct
    42301 gacgacgcgg agaaccggaa gctaaataca gagaagtcat agaaaccttt attgcactgc
    42361 aaactgctga cggactttac gcatcagcaa gaaaatccac ggccagagca ccccattgac
    42421 tacactactc cagaacactt ccggtctgaa agagacgtta atcactaaaa actctgccca
    42481 gaaaacaata atatccacca ccagcgaaag caacatgacg accagcgcct gctgccataa
    42541 tgcgaggttg cggaaaagct ggtatttcag cgccaccagg taagcaatga tgctcatcgc
    42601 caatacgcgt acgccaagcg tcgagccgct gatcagatcc agtatggcac ccatcacaaa
    42661 acctgtgccc acatttacgc gatgaggcaa ggccaggatc caatacaaca agatgagtaa
    42721 cacccagttt ggccggaaaa caatcaggtt atccggccag ggcatgattt gcagcaacag
    42781 cgcaatgagg aaagagagcc agattaccca acgtccctgg ctacgatagc tcgccactat
    42841 tgccctcccg gcgcacgcgc aggcggttga gcaccacttt gcggcggcgt agccctttgt
    42901 ggagagcggt tagcagcagg ctgtgtcggc gcagcaggcg cagtagctgc atttcctgtc
    42961 gtcggttgct gcggagtcgg ctgagcgatc cccgttgccg gttcaggtaa ctttggcccc
    43021 atcgcgtctg gcgaaggcaa tacctgcggc atcatctgca tcagacgttc attagcaaca
    43081 cgatgcacct cttccggcgt catcgggtta gcgccgttac gatccgcgcc ccacagcagc
    43141 agcagataac gcaaacgttg cagccctgct gtcggacgcg cctgaatcac agtataagcg
    43201 cgctgggtat cgagttttac ggaagagaca accgcgaccg gatagccttc cgggaaacga
    43261 ccgcccagac cggaagtcac cagcacatca ccaacacgaa tatccgtatt cgctggtaga
    43321 tgctcaagct gcaaatcatc cgtacaaccg ttaccggctg caattacgcg gatatcgttg
    43381 cgcagcacct ggattggcag cgcgtgggtc gcatcacaaa tcagcagcac gcgactggtc
    43441 agtttagcga cggccaccac ctgaccaaca acacctttgt cgctgatgac cggctggcct
    43501 tcataaacgc cattaacgct acctttatcg ataacaactt gatcgctata aggatcgtta
    43561 accgtggaga taacctgagt caccattttc tgctcatcct gacgcagcgg ggaacccagc
    43621 agctcgcgca gacgcgcgtt ctcctgtttg tattgtccaa gcatcagcag ttcactgttt
    43681 ttcagcaaca gttcctgacg taacgcccgg ttttcaagtt ctagttggtc acgcgaggcc
    43741 agcgtctgcg atacgccatc cagcaattca cgaggagcat tggaaacaaa gtagaaagga
    43801 ctgacggcgg tatccatata agtacggatt tgactgaacg tccccaggcg gctgtcggca
    43861 ataataatgc cgagcgccac cagcaccgcc agaataaggc gaatctgtag cgacgggcca
    43921 cggctaaaaa ttggcttcat aagttatgcg tattctcgta tcagaccagg cagggtaaac
    43981 agacatttcc cctgcctgca tccgattact cttcgctgaa caggtcgccg ccgtgcatgt
    44041 cgatcatttc cagcgctttg ccgccaccgc gcgccacaca ggtcagcggg tcttcagcaa
    44101 caacgactgg aatgccggtt tcttccatta acaaacggtc aaggttacgc agcagtgcgc
    44161 caccaccggt gagcaccatg ccgcgctcgg agatgtcgga agccagttcc ggcgggcact
    44221 gttccagtgc aaccattacc gcgctcacaa taccggtcag cggttcctgc agtgcttcga
    44281 ggatttcgtt ggagttcagg gtaaaaccgc gtggaacacc ttctgccagg ttacggccac
    44341 gaacttcgat ttcacggact tcatcgcccg ggtaagccga accgatttcg tgcttgatac
    44401 gttctgcggt ggcttcaccg atcagagaac cgtaattacg acgcacatag ttgatgatag
    44461 cttcgtcgaa acgatcacca ccaatgcgca cggaagagga gtaaaccacg ccgttcaagg
    44521 agataacagc aacttcagtg gtaccaccac cgatatcaac caccatagaa ccggtcgctt
    44581 cagaaaccgg caggccagca ccaattgcgg cagccatcgg ttcttcaatc aggaagactt
    44641 cacgggcacc agcgccctgc gcggattcac gaattgcgcg gcgttcaacc tgggtcgcgc
    44701 caaccggtac acaaaccaga acgcgcgggc ttggacgcat aaagctgttg ctgtgcactt
    44761 gtttgatgaa gtgctggagc attttttcag tcacgaagaa gtcggcgata acgccgtctt
    44821 tcattgggcg aatggcagca atattgcccg gcgtacggcc cagcatctgc ttcgcgtcat
    44881 gacctactgc agctacgctt ttcggtgaac cggcacgatc ctgacgaatg gccaccacgg
    44941 aaggctcatt caatacgatg ccttgtcctt ttacataaat gagggtattc gcagtaccca
    45001 ggtcaatgga caagtcattg gaaaacatgc cacgaaattt tttcaacata ctaagggata
    45061 atcctgaaag ctggggcgga aaagaaaatc cgcttacttt accaaccaca cgcagcagcg
    45121 acaaggcgca aaaatcatct gctacggtga aaattagtgc agttcgtttc ctttgttaca
    45181 aatctctgcc tgagtccaga aaggcttaat gcatcagcag cattcctcgc ctgtttgcaa
    45241 ccgcgtaagg tcattcatct gcatatgtgc tgcaacaatc tggcgagcag acaagcacac
    45301 tcccatgaga cgcagcgcgc gttattctac gtgaaaacag attaaacggc aggttaaacc
    45361 gagtatcttt gtgaatattt tttcacgtta gtatcaagtg gctgtgagga cgcgaaaaaa
    45421 tccccttgcc cgcctgtaac accgcgctga atcagggtct gccactcgct tcgcgaacgc
    45481 acaccggtgg cgtaaacttg ggtgctggtc ccggagcagg cttccaccag gctttgaacc
    45541 agcagctggt tctccgttcg cttctcaatg tttctgacca gccccggatg gagcttgagt
    45601 aactcaacat taagttcttt gatccaactg gtgcttacca gcgtcaaacc agcctggttg
    45661 acggctaccc gtacccctaa agcattcact aaacgaataa caggttgtaa acgactgata
    45721 tgttgaccta catcggcctc tgcaagttca ataattatgc gttttcgttg tgatttttca
    45781 cattgcatta acgtatcgcg cagccaacgc tgaaaacgcg ggcgaatcag cgactcaacg
    45841 gtaacctgaa tcgccagatt ttcctctggc cagtaacgca atagtggaat aagacggctg
    45901 atttgcagac ggtcatactc ttccgataag ccaaactgca agaccatcgg catatactcc
    45961 gccgagctaa cctcttcatt accatcgaag atgcggcaca tgagttcgcg atgatgaacc
    46021 tgaccttcgc gagtaaccgc cggtttttga taaaggcgcg ggccgccgcg actgagcatt
    46081 tgctcgataa gcgtacgcca gcgaacatta ccgcgtcctt tttcaggcaa cgagtcatcg
    46141 taaatagccc agctattgcc gccctgcaat cccgcattac gcgtggcaga ctctgcatgt
    46201 tccattacct gctcggtatc ctgaccacta cgccaggcgc agataccaat gtggatcata
    46261 tcgtcgcgat cgagcatttt attgttcggc aaggtatcga cggctttgat taactgaccg
    46321 gcgatgctct ctgcctcttt taacgtccgg tgcggtaaca gcgcagcaaa atcactgcgg
    46381 tggtaacgcg ccagcagtgc gccagggtag cgcatcataa atgtcgacag cagattcgtc
    46441 agagtgaaga actgttcttc aacctggctg tgcccccagg tatcgctcaa catattgaaa
    46501 tccggcagac gaatcatcat cacgatcccg tgggtaccta ctttctcctg atcttccagt
    46561 aacgttgcta actgattatc gaaaaagagt cggttattga ggccggtttt cacgtcctgg
    46621 gcggcataag agcggatcag cgtatcaaga cggctgtgtt gttcgcgtgc gttctgaatt
    46681 tcacgaagca gcgtatctag cgcactgctg gttctgggcg gccattcata gatggttccc
    46741 aacacattag agccacgctc accgtttaag atacgagtag cccgggtttc cagcaattct
    46801 tgcccggcaa gttgccgttg taaccagcgt accgccagga agagcataac aatgataaag
    46861 ccaatcgccc ccgtgagcgg cgcggtagtc attaaagaat ggaaatagtt gcccatcgga
    46921 tcctgataaa ccagacgcag cgacatcccc ggatgcttta tcaacggaac actcagttcg
    46981 cgaaacagat cgctggagcc aaccggacga taactaccat ttctggccag ggtataaacc
    47041 tgtttatcac catggagcag gtctacacga acgatatctg ccgacatcat taattcggta
    47101 atttgtggcc ttaatgtgct gaagtcattc gacacaaggt gggtatcaat cgccgtcgcc
    47161 accgcctgaa cgcgatgact aaacttatac tgaattgcgt tgtagaaact tagcgaacag
    47221 cccagcaaag tcacaaaaat tgttaacccg gtgagcagcg taacaaaggc cgaaaatttc
    47281 gtcgttaatc tcatccttgt gttaactccg atagtgagga agcgggcata ctagcaaatc
    47341 agatttatct cgcaatttat tgcgcttcat cggctttgct tttccattag cgagtatagt
    47401 cttcagaaat tattttccaa tccatcatgc acatgaggac cacttatgca ggcgttactt
    47461 ttagaacagc aggacggcaa aactctcgca tcggtacaga cacttgacga aagtcgcctg
    47521 ccggagggcg atgtcacggt tgatgttcac tggtcgagcc tgaactataa agatgcgctg
    47581 gcgattaccg gtaagggaaa aatcatccgt aattttccga tgattcctgg gatcgatttt
    47641 gccggaactg tacgcaccag cgaagatccg cgttttcatg ccggtcagga ggtgttactc
    47701 actggctggg gcgttggtga aaaccactgg ggtgggctgg cggagcaggc gcgagtgaaa
    47761 ggtgactggc tggttgccat gccgcaaggg ctggacgcgc gtaaagcaat gattatcggt
    47821 actgccggtt ttaccgccat gctgtgtgtg atggcgctgg aagatgccgg tgttcgcccg
    47881 caggacgggg agattgtcgt gacgggtgcc agtggtggcg tcggcagtac cgccgtggcg
    47941 ctgctgcata agttgggtta tcaggtcgtt gccgtttccg gtcgcgaaag tacccatgaa
    48001 tacctgaaaa gtttaggtgc cagtcgtatt ctccctcgtg atgagtttgc cgaatcccgt
    48061 cctctggaaa aacaagtctg ggctggggca attgacactg ttggcgacaa agtgctggca
    48121 aaagtgctgg cgcaaatgaa ttacggcggc tgcgtggcgg cctgtggtct ggcgggtggt
    48181 tttactctgc caaccacggt catgccattt attctgcgta atgtccgttt gcaaggggtg
    48241 gattcagtaa tgacgccacc agaacgccgc gcacaagcct ggcagcgact ggtcgccgat
    48301 ttaccggaat cattctatac ccaggcggca aaagagatat ctctgtcaga ggcaccgaac
    48361 tttgccgagg ccatcattaa taaccagatc cagggtcgca cgctggtgaa ggttaactaa
    48421 ccatttagca aggaataata agagagggaa ctcattttga catcattatt caatcggtta
    48481 acgggaaaag cggttatccg gacagctttc gtcgaacacc ttggtcagga agttgtacaa
    48541 catcatccaa actggaaagt catgatttcg actgaccaca aattgatgcg cattgatact
    48601 cctctaaaca gccattattg atatgcctcc gtcgcctgtt aggtttatgg tgctttgcct
    48661 gggcgacact acgcttaacc tattactgat ttctggtccc atggggtgaa taatctgatt
    48721 ttatttgact acaaattaac cactcgaacc tatttaatgc tgagcattgt cagtcggttg
    48781 attttgcgtg ctttagcatt cacatctatc cagacgatgc agtgaaaatt gggtaatccc
    48841 cagcaaccgc tgcgtaatgt cgtctatctt gtcgcgatcc tggcatccct acattatttg
    48901 tggtctgtga agattatctc attgcagccc ctcatcttcg cagggctggc tttacagctt
    48961 ttcaccttac gttataagaa gttccgtcga tgatggcgct aatttcgtga attgtgcggc
    49021 ttgttgcaaa ttacacggtg ttgaaggtta tttacatgtt agctgttgat tatcttccct
    49081 gataagacca gtatttagct gccaattgct acgaaatcgt tataatgtgc gacctcgtcc
    49141 tccctgacac agtttttgcg ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt
    49201 gttttttgcc ggaggatagc agcagatcgc tgcacaatgt ccgtcaagtc taacattgac
    49261 actctggggc aaaatagacc ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc
    49321 tgtcggggca tacgctttac agacggcggt gaaacgcctg tcacaatcac actaaacaaa
    49381 gagtacggaa cccactcatg gatattcgta agattaaaaa actgatcgag ctggttgaag
    49441 aatcaggcat ctccgaactg gaaatctccg aaggcgaaga gtcagtacgc atcagccgtg
    49501 cagctcctgc cgcaagtttc cctgtgatgc aacaagctta cgctgcacca atgatgcagc
    49561 agccagctca atctaacgca gccgctccgg cgaccgttcc ttccatggaa gcgccagcag
    49621 cagcggaaat cagtggtcac atcgtacgtt ccccgatggt tggtactttc taccgcaccc
    49681 caagcccgga cgcaaaagcg ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc
    49741 tgtgcatcgt tgaagccatg aaaatgatga accagatcga agcggacaaa tccggtaccg
    49801 tgaaagcaat tctggtcgaa agtggacaac cggtagaatt tgacgagccg ctggtcgtca
    49861 tcgagtaacg aggcgaacat gctggataaa attgttattg ccaaccgcgg cgagattgca
    49921 ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga ctgtcgctgt gcactccagc
    49981 gcggatcgcg atctaaaaca cgtattactg gcagatgaaa cggtctgtat tggccctgct
    50041 ccgtcagtaa aaagttatct gaacatcccg gcaatcataa gcgccgctga aatcaccggc
    50101 gcagtagcaa tccatccggg ttacggcttc ctctccgaga acgccaactt tgccgagcag
    50161 gttgaacgct ccggctttat cttcattggc ccgaaagcag acactattcg cctgatgggt
    50221 gacaaagtgt cggcaatcgc ggcgatgaaa aaagcgggcg ttccttgcgt accaggttct
    50281 gacggcccgc tgggcgacga tatggataaa aaccgtgcca ttgctaaacg cataggttat
    50341 ccggtgatta tcaaagcctc cggcggcggc ggcggtcgtg gtatgcgtgt agtgcgcggc
    50401 gacgctgaac tggcacaatc catctccatg acccgtgcgg aagcgaaagc cgcattcaat
    50461 aacgatatgg tttacatgga gaaatacctg gaaaatcctc gccacgtcga gattcaggta
    50521 ctggctgacg gtcagggcaa cgctatctat ctggcggaac gtgactgctc tatgcagcgt
    50581 cgtcaccaga aagtggtcga agaagcacca gcaccgggaa ttacaccgga actgcgtcgc
    50641 tacatcggcg aacgttgtgc taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact
    50701 ttcgagttcc tgttcgaaaa cggcgagttc tatttcatcg aaatgaacac ccgtattcag
    50761 gtagaacacc cggttacaga aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt
    50821 atcgctgccg gtcaaccgct gtcgatcaag caagaagaag ttcacgttcg cggccatgcg
    50881 gtggaatgtc gtatcaacgc cgaagatccg aacaccttcc tgccaagccc aggcaaaatc
    50941 acccgtttcc acgcacctgg cggttttgga gtgcgttggg agtctcatat ctacgcgggc
    51001 tacaccgtac cgccgtacta tgactcaatg atcggtaagc tgatttgcta cggtgaaaac
    51061 cgtgacgtgg cgattgctcg catgaagaat gcgctgcagg agctgatcat cgacggtatc
    51121 aaaaccaacg ttgatctgca gatccgcatc atgaatgacg agaacttcca gcatggtggc
    51181 actaacatcc actatctgga gaaaaaactc ggtcttcagg aaaaataaga ctgcaaaagc
    51241 gtcaaaaggc cggattttcc ggcctttttt attactgggg atcgacaacc cccataaggt
    51301 acaatcctcg ctttcttcac ccatcaggga caaaaaatgg acactcgttt tgttcaggcc
    51361 cataaagagg cgcgctgggc gctggggctg acccttttgt atctggcagt ttggttagta
    51421 gccgcttact tacctggcgt tgcccccggt tttaccggct ttccgcgctg gtttgagatg
    51481 gcctgcatcc tgacgccgct actgtttatt ggactgtgct gggcgatggt gaaatttatc
    51541 tatcgcgata tcccactgga ggatgacgat gcagcttgaa gtaattctac cgctggtcgc
    51601 ctatctggtg gtggtgttcg gtatctcggt ttatgcgatg cgtaaacgga gcaccggcac
    51661 cttccttaat gagtatttcc tcggcagccg ctctatgggc ggtattgtgc tggcgatgac
    51721 gctcaccgcg acctatatca gtgccagttc gtttatcggc gggccaggag ctgcttataa
    51781 atacgggctg ggctgggtat tgctggcgat gattcagctt cctgcagtct ggctttcact
    51841 cggtattctc ggcaagaagt ttgcgattct tgcgcgccgc tacaatgcag tgacgctgaa
    51901 cgatatgctg tttgcccgct accagagtcg tcttctggtg tggctggcga gtttgagttt
    51961 gctggttgcg ttcgttggtg cgatgaccgt gcagtttatc ggcggtgcgc gcctgctgga
    52021 aaccgcggcg ggtattcctt atgaaaccgg gctgctgatt tttggtatca gcattgcgtt
    52081 atataccgcc tttggtggct ttcgcgccag cgtgctgaac gacaccatgc aagggcttgt
    52141 gatgctgatt ggcaccgttg tgctgcttat tggcgtggta catgccgctg gcggcttaag
    52201 caacgcagta cagaccttgc aaaccatcga tccgcaactg gttacgccac aaggcgctga
    52261 cgatattctg tcgcctgcct ttatgacgtc gttctgggta ctggtgtgct ttggcgtgat
    52321 tggcctgccg catactgcgg tgcgctgtat ctcttataaa gacagcaaag cagtacaccg
    52381 ggggatcatc atcggtacga ttgtagtcgc aatcctgatg ttcggtatgc acctggccgg
    52441 agcgttaggt cgtgcagtga tccccgatct caccgtaccg gatctggtga tcccgacgtt
    52501 aatggtaaaa gtgctgccac cgtttgctgc cggaatcttc ctggctgcac ctatggctgc
    52561 gatcatgtcg acgatcaatg cccagttgct gcaaagttcc gctacgatca ttaaagatct
    52621 ctatctgaat atccgtccgg atcaaatgca aaacgagacg cgtctgaagc ggatgtcggc
    52681 ggtaattacg ttagttctcg gcgcgttgct gctgcttgcc gcctggaagc cgccagaaat
    52741 gatcatctgg ctgaatttgc tggccttcgg tgggctggaa gccgttttcc tgtggccgct
    52801 ggtgctgggt ctttactggg aacgcgccaa cgccaaaggc gcgctaagtg cgatgatcgt
    52861 tggcggcgtg ctgtatgccg tactcgcgac gctgaatatt cagtacctgg gcttccaccc
    52921 tattgtgccc tcgttactac taagtttgct ggctttcctg gtcggaaacc gtttcggtac
    52981 atccgtcccg caagctaccg ttttgactac tgataaataa agagttttgc catgccttgg
    53041 atccaactga aactgaacac caccggcgcg aacgcggaag atcttagcga tgcgctgatg
    53101 gaagcgggtg ccgtttctat cacttttcag gatacccacg atacgccagt atttgagccg
    53161 ctgccgggcg aaacgcgcct gtggggcgac accgatgtga ttggtctgtt cgacgctgaa
    53221 accgatatga acgacgtggt ggcgattctg gaaaaccatc cgctgctcgg cgcaggcttc
    53281 gcgcataaaa tcgaacaact agaagataaa gactgggagc gcgaatggat ggataatttc
    53341 cacccgatgc gctttggtga acgactgtgg atctgcccta gctggcgtga tgtgccggac
    53401 gaaaacgccg tcaacgtgat gttagatcca gggctggcgt ttggtacggg tacccatcca
    53461 accacctctc tgtgcctgca atggctcgac agcctcgatt taaccggtaa aacagtcatc
    53521 gactttggct gtggttccgg cattctggcg atcgcggcgc tgaaactggg tgcagcaaaa
    53581 gccattggta ttgatatcga tccgcaggcg attcaggcca gccgcgacaa cgccgaacgt
    53641 aatggcgttt ctgaccgtct ggaactctac ttaccgaaag atcagccaga agaaatgaaa
    53701 gccgacgtgg tggtcgctaa catccttgca ggcccattac gtgaactggc accgttaatc
    53761 agcgtcctgc cggtttcagg cggtttgctg ggcctttccg gtattctggc aagccaggca
    53821 gagagcgttt gtgaagctta tgccgatagc ttcgcactgg acccggtcgt ggaaaaagaa
    53881 gagtggtgcc gtattaccgg tcgtaagaat taaccttcgc atcgccgtag ggtgacgcgg
    53941 ggcgaagtgc gagcaagctc acaaaaggca cgtaaatttg ccggttattt acgcaaattt
    54001 gcgtgccaaa attttcattc ataaagaaaa attgagaact tactcaaatt tctttgagtg
    54061 taaattttag tcactatttt ctaatatgat gatttttatg agtaattatc gcaccacgct
    54121 cattttaaat gcaattcttt gatccatctc agaggattgg tcaaagtttg gcctttcatc
    54181 tcgtgcaaaa aatgcgtaat atacgccgcc ttgcagtcac agtatggtca tttcttaact
    54241 catgcgcatc ggacaatatc agctcagaaa tcgcctgatc gcagcgccca tggctggcat
    54301 tacagacaga ccttttcgga cgttgtgcta cgagatggga gccggattga cagtatccga
    54361 gatgatgtct tctaacccac aggtttggga aagcgacaaa tctcgtttac ggatggtgca
    54421 cattgatgaa cccggtattc gcaccgtgca aattgctggt agcgatccga aagaaatggc
    54481 agatgcagca cgtattaacg tggaaagcgg tgcccagatt attgatatca atatgggttg
    54541 cccggctaaa aaagtgaatc gcaagctcgc aggttcagcc ctcttgcagt acccggatgt
    54601 cgttaaatcg atccttaccg aggtcgtcaa tgcagtggac gttcctgtta ccctgaagat
    54661 tcgcaccggc tgggcaccgg aacaccgtaa ctgcgaagag attgcccaac tggctgaaga
    54721 ctgtggcatt caggctctga ccattcatgg ccgtacacgc gcctgtttgt tcaatggaga
    54781 agctgagtac gacagtattc gggcagttaa gcagaaagtt tccattccgg ttatcgcgaa
    54841 tggcgacatt actgacccgc ttaaagccag agctgtgctc gactatacag gggcggatgc
    54901 cctgatgata ggccgcgcag ctcagggaag accctggatc tttcgggaaa tccagcatta
    54961 tctggacact ggggagttgc tgcccccgct gcctttggca gaggttaagc gcttgctttg
    55021 cgcgcacgtt cgggaactgc atgactttta tggtccggca aaagggtacc gaattgcacg
    55081 taaacacgtt tcctggtatc tccaggaaca cgctccaaat gaccagtttc ggcgcacatt
    55141 caacgccatt gaggatgcca gcgaacagct ggaggcgttg gaggcatact tcgaaaattt
    55201 tgcgtaaaca gaaataaaga gctgacagaa ctatgttcga acaacgcgta aattctgacg
    55261 tactgaccgt ttctaccgtt aactctcagg atcaggtaac ccaaaaaccc ctgcgtgact
    55321 cggttaaaca ggcactgaag aactattttg ctcaactgaa tggtcaggat gtgaatgacc
    55381 tctatgagct ggtactggct gaagtagaac agcccctgtt ggacatggtg atgcaataca
    55441 cccgtggtaa ccagacccgt gctgcgctga tgatgggcat caaccgtggt acgctgcgta
    55501 aaaaattgaa aaaatacggc atgaactaat tcaggttagc taaatgcttg attaaaaagg
    55561 cgctactcgg catggggaag cgcctttttt ataggtgtca caaagggagt gaccatgaga
    55621 acaggatgtg aaccgacccg gtttggtaat gaagctaaga ccattattca cggtgatgcg
    55681 cttgccgaac ttaaaaagat ccccgccgaa agtgtcgatc tgatctttgc cgacccacca
    55741 tataacatcg gtaaaaattt tgatggtctg atcgaagcct ggaaagaaga tctgtttatc
    55801 gactggctgt ttgaagtgat tgcagagtgc caccgcgttc tgaaaaagca gggcagcatg
    55861 tacatcatga acagcacgga aaacatgccc tttatcgatc tccagtgccg caagcttttt
    55921 accatcaaaa gtcgcatcgt ctggtcatat gacagttctg gagtacaggc gaaaaaacac
    55981 tacggctcca tgtacgaacc catcctgatg atggtgaaag acgcaaagaa ctacacattc
    56041 aacggtgatg ctattctggt agaagccaaa accggatcgc agcgcgcgtt gatcgattat
    56101 cgcaaaaatc ctccacagcc atacaatcat caaaaagtac cgggtaacgt ctgggatttt
    56161 ccgcgcgtgc gttatttgat ggatgaatat gagaaccacc cgacgcaaaa accggaagcc
    56221 ttactgaaac gcattattct cgcctcttcc aacccaggcg atatcgttct cgacccgttt
    56281 gccggtagct ttactaccgg tgccgtagcc gtctccagcg ggcgaaaatt cattggtatt
    56341 gagatcaaca gcgagtacat caaaatgggg cttcgacggc tggatgtcgc gtcgcattac
    56401 tctgcggaag aactggcgaa agtgaaaaaa agaaagaccg gcaacctgtc aaaacgaagc
    56461 cggttaagcg aagttgaccc cgatctcatt gcaaagtaaa ggaagtgtaa gcctggtttt
    56521 cagattattc atttcgtgta tatttcctgc cagacttggt taaacataca caggctcagg
    56581 taatgattcg caagtattgg tggctcgtcg ttttcgctgt cttcgttttt ctgtttgata
    56641 ctttactgat gcagtggatt gaactgctgg caacagaaac agacaaatgc cgcaatatga
    56701 actcagttaa tccactaaaa ctggttaact gtgacgaact gaattttcag gacagaatgt
    56761 gaatttacat gacacttaat tcattcgttt gatgaattaa tttcgttatg ttttcatctg
    56821 gcatgaacat tcttaatacg ttatcgacca gagcgggggc ttgtttataa agatcataac
    56881 ccgccatatt cattaaccag ttttgaacaa ttccgctgaa ggcaccatta ataataatca
    56941 tcacaacatc taaatcgagg ttatttgcta cacaaccttg ttgctgacac gcctgcaata
    57001 cttcgcggag agtctgcgga ttgaagccca tcttttcgcg tatcactccc tcggccagca
    57061 tctcatcatt aaattcacat ttgtgatata agattttcag caacgcctgc tggcggggaa
    57121 ttttggcaat atattgcaag ccgacaatca atttttcacg caattgttga aacgggtcat
    57181 gctctaatcc agccgtcaag tgttcctgga ttaactcccg caatgaaggc tgttgcaacc
    57241 acatctcatt aaacagttga gtcttgtttt cgaagtgcca gtagatagcg ccacgcgtaa
    57301 cgttagcagc gtcggcaatg tcgttgagtg tcgtcttgct tacgccatgc tgcgcaaact
    57361 gggcgatggc agtttcaatc agttcttgcc gggtcttcag agcttcggct ttggttcttt
    57421 ttgccatgat taattattca ggaaataaat atattcgaca cagagtgaga aaatagcgaa
    57481 ggttaatcta tcacctaatg tgtatttata cgagaggcta atattgagtt gctataaatc
    57541 gtcaaataaa taatatatat tatttaccta aaatacattc gccacatgaa tatatatttc
    57601 aatttacgag gttttaattc tgcctctttc aacccgcgtc aaaataaaac agtagaatat
    57661 taatcttttt ttgtgtttat gtgccttgag acgccagtat tcataactat tccttacatc
    57721 gacgaatgat aatttgtagg atagcaaact gtatttttct ttctgcgagt taacgcgttg
    57781 cctttctggg taaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc
    57841 aggtttttcc tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac
    57901 gataagggag aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtgaaa
    57961 acggccccgt tagaagttaa gactgaatta ccaggccgca ccaatgctta ccgtatagcc
    58021 gaagttcgcc cacaggttag cgggatcgta ctgaatcgta atttcactga aggcagcgat
    58081 gtacaagcag gccagtccct gtaccagatc gatcccgcga cctatcaggc aaattatgac
    58141 agcgcgaaag gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta
    58201 aaacgttacg ttccgctcgt gggtacgaaa tacatcagcc agcaggagta cgaccaggcc
    58261 attgctgatg ctcgccaggc cgatgccgcc gtgattgccg caaaagccac agtcgaaagc
    58321 gctcgcatca atctcgctta taccaaagtg actgcgccaa ttagcggacg tatcggcaaa
    58381 tcgaccgtga ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcgactgtc
    58441 cagcagctcg atcctatcta cgttgatgtg acccaatcca gcaacgattt tatgaggctg
    58501 aagcaatccg tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg
    58561 gtcatggaaa acggtcaaac ctatcccctg aaaggtacgc tgcaattctc tgatgtgacc
    58621 gttgatgaaa gcaccggctc cataacccta cgtgccgtct tccctaaccc gcaacatacg
    58681 cttttgccgg gtatgtttgt gcgcgcacgg attgatgaag gcgtccaacc tgacgccatt
    58741 cttatcccgc aacaaggcgt tagccgcaca ccgcgtggtg atgcaaccgt gctgattgtt
    58801 aacgataaaa gtcaggtgga agcgcgccct gtcgttgcca gtcaggcgat tggcgataaa
    58861 tggttgatta gcgaaggtct gaaatctggc gatcaagtca ttgtcagcgg cctgcaaaaa
    58921 gcgcgtccgg gagagcaggt taaagccact accgataccc ccgcagatac tgcatcgaag
    58981 taaggtaatc tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg
    59041 ccattattct gatgatggcg ggcgcactgg cgatcctaca attacccgtc gctcagtatc
    59101 caaccattgc accgcctgcg gtttctgttt cagcaaacta tccgggcgct gatgcgcaga
    59161 ccgtgcagga tacggtgacg caggttatcg aacagaatat gaacggtatc gataacctga
    59221 tgtatatgtc ctccaccagc gattccgccg gtagcgtgac aattaccctt accttccagt
    59281 ccgggaccga tcctgatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc
    59341 cgttgctgcc gcaggaggtt cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct
    59401 atttgatggt ggcgggcttt gtctctgata acccagacac cacacaggac gatatctcgg
    59461 actatgtggc ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc ggtgacgtac
    59521 agcttttcgg cgcacagtat gcgatgcgta tctggctgga cgccgatctg ctaaacaaat
    59581 ataaactgac accggttgat gtgattaacc agttgaaggt acagaacgat cagatcgctg
    59641 ccggacagtt gggcggaacg ccagcgttac cagggcaaca gttgaacgcc tcaattattg
    59701 ctcagacgcg gttaaaaaat ccggaagaat tcggcaaagt aaccctgcgc gtaaacagtg
    59761 acggctcggt ggtacgcctg aaagatgtcg cacgagttga acttggcggt gaaaactata
    59821 acgttatcgc tcgtatcaac ggaaaaccgg cggcgggcct ggggattaag ctggcaaccg
    59881 gcgcgaatgc tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat
    59941 tcttcccgca gggaatgaag gttctctacc cttatgacac cacgccattc gtccagcttt
    60001 ctattcacga agtggtaaaa acgctgtttg aagccattat gctggtgttc ctggtgatgt
    60061 atctgttctt gcagaatatg cgagcaacgc tgatccccac cattgcggta cccgtggtgt
    60121 tattagggac gtttgccatc ctcgccgctt ttggttactc catcaacaca ctaacgatgt
    60181 tcgggatggt gcttgccatc gggctgctcg tcgatgatgc gatagtggtg gtggagaacg
    60241 tcgagcgcgt gatgatggag gataagctcc cgccaaaaga agcgacggaa aaatcaatgt
    60301 cgcaaatcca gggcgcactg gtgggtatcg cgatggtgct gtcagcggta tttattccga
    60361 tggcattctt cggcggttct actggggcaa tttatcgcca gttctctatc accatcgttt
    60421 cggcaatggc gctttctgtt ctggtggcat tgattcttac ccctgcgtta tgtgcaacgc
    60481 tgcttaaacc cgtctctgct gagcatcacg aaaataaggg cggtttcttc ggctggttta
    60541 atactacctt cgatcatagc gttaaccact acaccaacag cgtcggcaaa atcctcggtt
    60601 ccacaggacg atatttactg atctatgcgc tgattgttgc aggaatggtg gtgttgtttt
    60661 tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg accatgattc
    60721 agttacccgc tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa gtgacggatt
    60781 actatctgaa gaacgagaaa gcgaacgttg aaagtgtctt tacggttaac ggctttagct
    60841 tcagcggcca ggcacaaaat gccggtatgg ccttcgtcag tctgaaaccg tgggaagagc
    60901 gtaatggtga tgaaaacagt gcggaagcgg taatccatcg tgccaaaatg gaatttggca
    60961 agatccgcga cggttttgtc attccattca atatgccagc cattgttgaa ctgggcacgg
    61021 caacgggttt cgactttgag ttaattgatc aggctgggct gggtcacgat gccctaaccc
    61081 aggcccgtaa ccagttgctt ggtatggcgg cgcaacatcc tgccagctta gtcagcgtgc
    61141 gtcctaatgg cctggaagac accgcgcagt ttaaactgga agttgaccag gaaaaggcgc
    61201 aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacggcg ttgggtggga
    61261 cttacgttaa cgacttcatc gaccgtggtc gcgtgaaaaa ggtgtatgtt caggcggatg
    61321 ccaaattccg tatgctgcca gaagatgtcg ataaacttta tgtccgcagc gccaacggcg
    61381 aaatggtgcc attctcggcc tttaccactt cacattgggt gtatggctct ccgcgactgg
    61441 aacgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca ggaaccagtt
    61501 ccggcgatgc catggcgttg atggaaaacc ttgcgtcaaa attacctgct ggcattggtt
    61561 atgactggac gggtatgtcg tatcaggaac gcttatcggg aaaccaggct cccgctctgg
    61621 tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa agctggtcaa
    61681 ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgctg ctggcggcga
    61741 cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg acaattggct
    61801 tgtcggccaa aaacgctatt ttgattgttg agttcgctaa agatctcatg gagaaagagg
    61861 gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat gcgtctgcgt cctatcctga
    61921 tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac ggtgccggca
    61981 gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg aatggtctct gcaacgttgc
    62041 tggcaatctt cttcgtaccg gtgttctttg tggtgatccg ccgttgcttt aaaggataaa
    62101 tcagaaacat aaaggcactt tcgggtgcct ttattatttt cagtgaaacc cataaaaatt
    62161 aataagatat tcttccactc actctttaaa aactttctat agttcccgct cccttcacta
    62221 tttttacaat tcacataatt tgtcattatt cattccgcaa tatttataac ctccggtgat
    62281 aaaatggcat tcagctcgtt aataagagag ttaacttatt aagcgttagc gtttattact
    62341 gaggtaacac catgaaaaga ttaattcctg tggcattgct caccgcattg ctggcgggct
    62401 gtgctcacga ttcgccctgc gttccggttt atgacgatca gggtcgtctg gttcacacca
    62461 atacctgtat gaaaggcacg actcaggata actgggaaac ggctggggct attgccggcg
    62521 gggcagcggc tgttgctggc ctgacgatgg ggatcatcgc tctgtcgaaa taactgaaca
    62581 gggcgcggag tttcgcgctc tgtttaacga tacagtatct ttacataaga taaaatattg
    62641 ctatattctg ctggctaatt ctctgctgat ttagcatgtt atatcgcgtt atttcgctgc
    62701 tttcgaaata acgcatcaat ttaactttca cacttcccac cagaaatata ctcacgctaa
    62761 caatcattat tctgcttagt aaaacacgcg taaatatttt gcgccaaaat gtggcgcatg
    62821 tttcattttc gcaccattgc ggggcgctgt ttttattatt tcctgtctac actcagctta
    62881 ttgcgacgcg aaatcgggcg caaaagctgg cactactttt gcttataaga ggatggccac
    62941 agacaggtaa aagacgtttc ccaaacgtcc tataacgata attttcgcct cacaggaagc
    63001 attatgaaaa agatgatgat agccacactg gctgccgcca gcgtgctgct tgccgttgca
    63061 aatcaggcgc atgctggcgc gacgcttgat gccgttcaga aaaaaggttt tgtgcaatgc
    63121 gggatcagtg atggattacc tgggttctct tatgccgatg cagacggtaa gttttcaggt
    63181 attgatgttt gtcgtggtgt tgccgctgct gtatttggtg acgacacgaa agtgaaatat
    63241 accccactca cagcaaaaga acgcttcacg gctttacagt caggggaagt ggatttgctc
    63301 tcccgtaata cgacctggac ttcatctcgc gacgccggga tgggaatggc atttactggc
    63361 gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct aaaaagcgcg
    63421 aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga actcaacgtt
    63481 gccgactatt tcaaggcaaa caatatgaag tacacaccgg tgactttcga tcgctctgac
    63541 gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga tcagtcgcaa
    63601 ctgtatgccc tgcgcatcaa attaagcaac ccagctgaat ggattgtctt accggaggtt
    63661 atctctaaag aaccgcttgg gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt
    63721 gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa ttcccagaac
    63781 gtcgatgaaa aagcggctaa tccagcaacg cctgatatgg cacatctgct gggtaaagaa
    63841 ggcgattacg gcaaggatct gaagctggat aataaatggg cctataacat catcaaacag
    63901 gtgggtaact actcggaaat tttcgagcgt aacgtaggtt cagaaagccc gctgaaaatt
    63961 aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc gcccgtgcgt
    64021 taagtcggtg tgaggtaacg ggcgtcgcta cggcggcgct ctcttcagcg ttatgctttc
    64081 cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctctt ttgccaaccc
    64141 tacggttcgc gcctggttat tccagatcct tgccgttgtt gctgttgtcg gcattgttgg
    64201 ttggttattt cacaacactg taacgaatct caataatcgt ggcattactt caggttttgc
    64261 ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt accagcaggg
    64321 cgacacctac ggacgcgttt ttattgtcgg cttactcaat acgctactgg tttctgcatt
    64381 gtgtattgtg ttcgcttctg ttttaggctt ctttatcggt ctggcgagac tttcggataa
    64441 ctggctgcta cgaaaactat ccacaattta tattgagatc ttccgtaata ttcccccgct
    64501 gctgcaaatc ttcttctggt actttgccgt gttgcgcaat ttgcccggac cacgccaggc
    64561 tgtgagcgcg tttgatctgg cctttttgag caatcgtggg ctttatattc cgtcaccgca
    64621 gctgggagac ggatttattg cgtttatcct ggctgttgtt atggctatag tcctttctgt
    64681 tgggctattc cgctttaata aaacatacca gataaagacc ggacaactgc gccgcacctg
    64741 gccgatcgcc gcagtgttga tcattggttt gcctttactg gcgcaatggc tttttggcgc
    64801 agcactacac tgggatgtcc cagccctacg aggctttaac ttccgcggcg ggatggtttt
    64861 aattcctgaa ctggcagcct taacgctggc actttcggtt tatacatctg catttatcgc
    64921 cgagattatc cgcgctggga tccaggcagt gccttatggt caacatgaag cggctcggtc
    64981 actgggatta cccaacccgg ttacgctacg ccaggtcatt attccccagg cactgcgagt
    65041 gattattccg ccgttaacca gccagtatct caacatcgtc aaaaactcct ctcttgccgc
    65101 cgctattggc tatcccgata tggtttcgct gtttgctggc accgtgctga accagacggg
    65161 gcaagccatc gagacgatag ccatgaccat gtcggtctat ctgattatca gcctgactat
    65221 ctcgctgctg atgaatatct ataaccgccg catcgcgatc gttgaacgct aaggagtcat
    65281 gatgacaaaa gtattgctgt ctcagccctc gcgcccagcg agccataact caagccgcgc
    65341 gatggtgtgg gtgcgaaaaa atctgttctc cagctggagc aatagcctgc tgactattgg
    65401 ctgcatatgg ttgatgtggg aactgatccc accgttgctg aactgggcat ttttgcaggc
    65461 taattgggtt ggctcaacgc gtgccgactg cacaaaagcc ggtgcctgtt gggtcttcat
    65521 ccacgaacga tttggtcagt ttatgtatgg actttaccca cacgaccaac gctggcgaat
    65581 taacctggca ttactgattg gccttgtgtc aatcgcacca atgttctgga aaatactccc
    65641 gcatcgcggt cgctatattg cggtctgggc ggtgatttac ccactgattg tctggtggct
    65701 gatgtatggc ggtttttttg gtctggagcg ggttgaaacc cggcaatggg gcgggctgac
    65761 gctaacttta attattgcat ccgttgggat tgcgggggcg ctaccgtggg ggatcttact
    65821 ggcgttaggt cgccgctccc atatgccgat tgtgcgtatc ttatcggtca tttttatcga
    65881 gttctggcgc ggcgtaccgc tgattaccgt attgtttatg tcttcggtca tgctgccttt
    65941 gtttatggca gaaggcacca atatcgacaa actgatccgc gcgctggttg gcgtgatcct
    66001 gtttcagtca gcatatgttg cggaagtcgt gcgaggcgga ttacaggcgc tgcctaaagg
    66061 gcaatatgaa gcggcagagt cgctggcgtt gggttactgg aaaactcagg ggctggttat
    66121 tctgccacag gcgttgaagc tggtaattcc tgggctggta aataccatca tcgcactctt
    66181 caaagatacc agcctggtga tcattatcgg gttgttcgat cttttcagta gcgttcagca
    66241 ggcaaccgtt gatcccacct ggttgggtat gtcgacggaa gggtatgttt tcgccgcgct
    66301 gatctactgg atcttctgtt tcagcatgtc gcgctatagc cagcatctgg aaaaacgttt
    66361 taacaccggg cgtacaccgc attgaggaca ctatgagcca aattttactg caacctgcta
    66421 acgcgatgat tacgctggaa aacgtcaata aatggtatgg acaattccat gttttgaaaa
    66481 atataaattt aaccgtgcaa ccgggagaac ggatcgttct gtgtggccct tcaggttccg
    66541 gtaaatcgac aaccattcgt tgtattaatc atctggaaga acatcaacag ggacggatcg
    66601 tggtagatgg catcgaactt aatgaagata tccgcaatat tgagcgcgtc cggcaggaag
    66661 tgggaatggt ctttcagcat ttcaatctct tccctcatct gaccgttcta cagaactgta
    66721 ccctggcacc gatttgggta cgcaagatgc caaagaaaga ggctgaagct ctggcgatgc
    66781 attaccttga gcgggtgaga attgccgaac atgcgcataa gtttcccgga caaatttctg
    66841 gtggtcagca gcaacgcgtt gccattgcgc gttcgctgtg tatgaagccg aaaattatgt
    66901 tgtttgatga gccaacgtcg gcgctcgatc cggagatggt gaaagaggtg ctggatacga
    66961 tgattgggct ggcgcaatcg ggtatgacaa tgttgtgtgt aacgcatgag atggggtttg
    67021 cgcgaaccgt cgctgaccgg gtgattttta tggatcgtgg ggaaatagtg gagcaagctg
    67081 cacctgatga attttttacg catcctaaat cagagcgtac gagggcattt ttatcgcagg
    67141 taatccatta attgaatgtt agttcgaaaa gcaaaaaggc catcctttcg gatggccttt
    67201 ttgcttgatt tgatgtctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
    67261 ttgcttcgca acgttcaaat ccgttcccgg cggatttgtc ctggttggga gagtgttcac
    67321 cgacaaacat caaataaaac aaaaggccca gtcttccgac tgagccttat gttttatttg
    67381 atg
//
LOCUS       DAHBHC010000017        66573 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHBHC010000017 DAHBHC010000000
VERSION     DAHBHC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.017"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..448
                     /locus_tag="M2A35_001443"
     CDS             <1..448
                     /locus_tag="M2A35_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCB7197762.1"
                     /translation="EFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRA
                     IGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVET
                     QGKYSQGMTVVDYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            532..2202
                     /gene="hscC"
                     /locus_tag="M2A35_001444"
     CDS             532..2202
                     /gene="hscC"
                     /locus_tag="M2A35_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCB7197763.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLCSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(2262..3713)
                     /gene="djlC"
                     /locus_tag="M2A35_001445"
     CDS             complement(2262..3713)
                     /gene="djlC"
                     /locus_tag="M2A35_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCB7197764.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEESEAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(3710..4417)
                     /locus_tag="M2A35_001446"
     CDS             complement(3710..4417)
                     /locus_tag="M2A35_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCB7197765.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEDSLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            4518..5072
                     /locus_tag="M2A35_001447"
     CDS             4518..5072
                     /locus_tag="M2A35_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCB7197766.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(5082..6509)
                     /locus_tag="M2A35_001448"
     CDS             complement(5082..6509)
                     /locus_tag="M2A35_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCB7197767.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLWWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(6506..7213)
                     /locus_tag="M2A35_001449"
     CDS             complement(6506..7213)
                     /locus_tag="M2A35_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCB7197768.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            7377..8354
                     /locus_tag="M2A35_001450"
     CDS             7377..8354
                     /locus_tag="M2A35_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCB7197769.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(8424..8906)
                     /locus_tag="M2A35_001451"
     CDS             complement(8424..8906)
                     /locus_tag="M2A35_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCB7197770.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFIRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            9141..11723
                     /gene="leuS"
                     /locus_tag="M2A35_001452"
     CDS             9141..11723
                     /gene="leuS"
                     /locus_tag="M2A35_001452"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCB7197771.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            11738..12319
                     /gene="lptE"
                     /locus_tag="M2A35_001453"
     CDS             11738..12319
                     /gene="lptE"
                     /locus_tag="M2A35_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCB7197772.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            12319..13350
                     /gene="holA"
                     /locus_tag="M2A35_001454"
     CDS             12319..13350
                     /gene="holA"
                     /locus_tag="M2A35_001454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCB7197773.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRSMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            13352..13993
                     /gene="nadD"
                     /locus_tag="M2A35_001455"
     CDS             13352..13993
                     /gene="nadD"
                     /locus_tag="M2A35_001455"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCB7197774.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            14017..14628
                     /locus_tag="M2A35_001456"
     CDS             14017..14628
                     /locus_tag="M2A35_001456"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCB7197775.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            14888..15205
                     /gene="rsfS"
                     /locus_tag="M2A35_001457"
                     /gene_synonym="rsfA"
     CDS             14888..15205
                     /gene="rsfS"
                     /locus_tag="M2A35_001457"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCB7197776.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            15209..15676
                     /gene="rlmH"
                     /locus_tag="M2A35_001458"
     CDS             15209..15676
                     /gene="rlmH"
                     /locus_tag="M2A35_001458"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCB7197777.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            15707..17608
                     /gene="mrdA"
                     /locus_tag="M2A35_001459"
     CDS             15707..17608
                     /gene="mrdA"
                     /locus_tag="M2A35_001459"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCB7197778.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            17611..18723
                     /gene="mrdB"
                     /locus_tag="M2A35_001460"
                     /gene_synonym="rodA"
     CDS             17611..18723
                     /gene="mrdB"
                     /locus_tag="M2A35_001460"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCB7197779.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            18734..19822
                     /gene="rlpA"
                     /locus_tag="M2A35_001461"
     CDS             18734..19822
                     /gene="rlpA"
                     /locus_tag="M2A35_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCB7197780.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            19962..21173
                     /gene="dacA"
                     /locus_tag="M2A35_001462"
     CDS             19962..21173
                     /gene="dacA"
                     /locus_tag="M2A35_001462"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCB7197781.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            21284..21547
                     /locus_tag="M2A35_001463"
     CDS             21284..21547
                     /locus_tag="M2A35_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCB7197782.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            21648..22289
                     /gene="lipB"
                     /locus_tag="M2A35_001464"
     CDS             21648..22289
                     /gene="lipB"
                     /locus_tag="M2A35_001464"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCB7197783.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIVPRLLENILALLNNPDFEYITA"
     gene            22548..23501
                     /locus_tag="M2A35_001465"
     CDS             22548..23501
                     /locus_tag="M2A35_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCB7197784.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINLTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            23710..24675
                     /gene="lipA"
                     /locus_tag="M2A35_001466"
     CDS             23710..24675
                     /gene="lipA"
                     /locus_tag="M2A35_001466"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCB7197785.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(24776..24979)
                     /gene="tatE"
                     /locus_tag="M2A35_001467"
     CDS             complement(24776..24979)
                     /gene="tatE"
                     /locus_tag="M2A35_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCB7197786.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(25108..25896)
                     /locus_tag="M2A35_001468"
     CDS             complement(25108..25896)
                     /locus_tag="M2A35_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCB7197787.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            25989..26372
                     /gene="crcB"
                     /locus_tag="M2A35_001469"
     CDS             25989..26372
                     /gene="crcB"
                     /locus_tag="M2A35_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCB7197788.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     TFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(26427..26636)
                     /gene="cspE"
                     /locus_tag="M2A35_001470"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(26427..26636)
                     /gene="cspE"
                     /locus_tag="M2A35_001470"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCB7197789.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(26811..27371)
                     /gene="pagP"
                     /locus_tag="M2A35_001471"
     CDS             complement(26811..27371)
                     /gene="pagP"
                     /locus_tag="M2A35_001471"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCB7197790.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            27959..29344
                     /gene="dcuC"
                     /locus_tag="M2A35_001472"
     CDS             27959..29344
                     /gene="dcuC"
                     /locus_tag="M2A35_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCB7197791.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(29385..30065)
                     /gene="dpiA"
                     /locus_tag="M2A35_001473"
                     /gene_synonym="citB"
     CDS             complement(29385..30065)
                     /gene="dpiA"
                     /locus_tag="M2A35_001473"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCB7197792.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(30034..31692)
                     /gene="dpiB"
                     /locus_tag="M2A35_001474"
     CDS             complement(30034..31692)
                     /gene="dpiB"
                     /locus_tag="M2A35_001474"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCB7197793.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            32072..33130
                     /gene="citC"
                     /locus_tag="M2A35_001475"
     CDS             32072..33130
                     /gene="citC"
                     /locus_tag="M2A35_001475"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCB7197794.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRRTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            33145..33441
                     /gene="citD"
                     /locus_tag="M2A35_001476"
     CDS             33145..33441
                     /gene="citD"
                     /locus_tag="M2A35_001476"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCB7197795.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            33438..34346
                     /gene="citE"
                     /locus_tag="M2A35_001477"
     CDS             33438..34346
                     /gene="citE"
                     /locus_tag="M2A35_001477"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCB7197796.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            34357..35889
                     /gene="citF"
                     /locus_tag="M2A35_001478"
     CDS             34357..35889
                     /gene="citF"
                     /locus_tag="M2A35_001478"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCB7197797.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            35893..36444
                     /gene="citX"
                     /locus_tag="M2A35_001479"
     CDS             35893..36444
                     /gene="citX"
                     /locus_tag="M2A35_001479"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCB7197798.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            36419..37297
                     /gene="citG"
                     /locus_tag="M2A35_001480"
     CDS             36419..37297
                     /gene="citG"
                     /locus_tag="M2A35_001480"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCB7197799.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            37348..38811
                     /gene="citT"
                     /locus_tag="M2A35_001481"
     CDS             37348..38811
                     /gene="citT"
                     /locus_tag="M2A35_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCB7197800.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANSLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            38925..39731
                     /gene="rna"
                     /locus_tag="M2A35_001482"
     CDS             38925..39731
                     /gene="rna"
                     /locus_tag="M2A35_001482"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCB7197801.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            39961..40371
                     /gene="rnk"
                     /locus_tag="M2A35_001483"
     CDS             39961..40371
                     /gene="rnk"
                     /locus_tag="M2A35_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCB7197802.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(40602..41840)
                     /locus_tag="M2A35_001484"
     CDS             complement(40602..41840)
                     /locus_tag="M2A35_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCB7197803.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            42061..42489
                     /gene="uspG"
                     /locus_tag="M2A35_001485"
     CDS             42061..42489
                     /gene="uspG"
                     /locus_tag="M2A35_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCB7197804.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(42610..44175)
                     /gene="ahpF"
                     /locus_tag="M2A35_001486"
     CDS             complement(42610..44175)
                     /gene="ahpF"
                     /locus_tag="M2A35_001486"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCB7197805.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(44304..44867)
                     /gene="ahpC"
                     /locus_tag="M2A35_001487"
     CDS             complement(44304..44867)
                     /gene="ahpC"
                     /locus_tag="M2A35_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCB7197806.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            45239..45985
                     /gene="dsbG"
                     /locus_tag="M2A35_001488"
     CDS             45239..45985
                     /gene="dsbG"
                     /locus_tag="M2A35_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCB7197807.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDSAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            46194..47095
                     /locus_tag="M2A35_001489"
                     /pseudo
     CDS             46194..47095
                     /locus_tag="M2A35_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            47242..48462
                     /locus_tag="M2A35_001490"
     CDS             47242..48462
                     /locus_tag="M2A35_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCB7197808.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            48435..49064
                     /locus_tag="M2A35_001491"
     CDS             48435..49064
                     /locus_tag="M2A35_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCB7197809.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(49065..50225)
                     /gene="ybdL"
                     /locus_tag="M2A35_001492"
     CDS             complement(49065..50225)
                     /gene="ybdL"
                     /locus_tag="M2A35_001492"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCB7197810.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PHYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            50334..51422
                     /gene="hcxA"
                     /locus_tag="M2A35_001493"
     CDS             50334..51422
                     /gene="hcxA"
                     /locus_tag="M2A35_001493"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCB7197811.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(51432..51629)
                     /locus_tag="M2A35_001494"
     CDS             complement(51432..51629)
                     /locus_tag="M2A35_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCB7197812.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(51811..53916)
                     /gene="cstA"
                     /locus_tag="M2A35_001495"
     CDS             complement(51811..53916)
                     /gene="cstA"
                     /locus_tag="M2A35_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCB7197813.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(54097..54510)
                     /gene="entH"
                     /locus_tag="M2A35_001496"
     CDS             complement(54097..54510)
                     /gene="entH"
                     /locus_tag="M2A35_001496"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCB7197814.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(54513..55259)
                     /gene="entA"
                     /locus_tag="M2A35_001497"
     CDS             complement(54513..55259)
                     /gene="entA"
                     /locus_tag="M2A35_001497"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCB7197815.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(55259..56116)
                     /gene="entB"
                     /locus_tag="M2A35_001498"
     CDS             complement(55259..56116)
                     /gene="entB"
                     /locus_tag="M2A35_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCB7197816.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(56130..57740)
                     /gene="entE"
                     /locus_tag="M2A35_001499"
     CDS             complement(56130..57740)
                     /gene="entE"
                     /locus_tag="M2A35_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCB7197817.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTLVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(57750..58925)
                     /gene="entC"
                     /locus_tag="M2A35_001500"
     CDS             complement(57750..58925)
                     /gene="entC"
                     /locus_tag="M2A35_001500"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCB7197818.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            59300..60256
                     /gene="fepB"
                     /locus_tag="M2A35_001501"
     CDS             59300..60256
                     /gene="fepB"
                     /locus_tag="M2A35_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCB7197819.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(60260..61510)
                     /gene="entS"
                     /locus_tag="M2A35_001502"
     CDS             complement(60260..61510)
                     /gene="entS"
                     /locus_tag="M2A35_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCB7197820.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            61621..62625
                     /gene="fepD"
                     /locus_tag="M2A35_001503"
     CDS             61621..62625
                     /gene="fepD"
                     /locus_tag="M2A35_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCB7197821.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            62622..63614
                     /gene="fepG"
                     /locus_tag="M2A35_001504"
     CDS             62622..63614
                     /gene="fepG"
                     /locus_tag="M2A35_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCB7197822.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            63611..64426
                     /gene="fepC"
                     /locus_tag="M2A35_001505"
     CDS             63611..64426
                     /gene="fepC"
                     /locus_tag="M2A35_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCB7197823.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(64423..65556)
                     /gene="fepE"
                     /locus_tag="M2A35_001506"
     CDS             complement(64423..65556)
                     /gene="fepE"
                     /locus_tag="M2A35_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCB7197824.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(65772..>66573)
                     /locus_tag="M2A35_001507"
     CDS             complement(65772..>66573)
                     /locus_tag="M2A35_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCB7197825.1"
                     /translation="QVMVASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCF
                     HPASGFAWQFSVLSRYLDPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPH
                     GPYYLLGYSLGGTLAQGIAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVL
                     AEINREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTL
                     QEGMSPERAWSPWIAELDIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT        16455 a        17421 c        16489 g        16208 t
ORIGIN      
        1 tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc
       61 ggtggtgatg gccggtctgg atgttactca taaagcacaa atccacgttg aagacaccga
      121 gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt
      181 cctcgaatat cataaagacg aaaaatgggg ctttgtcggt gcaccgctgc atgacccatg
      241 caccatcgcc tggctgttga aacccgagtt gtttaccact gttgagcgct gggttggcgt
      301 ggaaacacag gggaaatata gccagggtat gacggttgtt gattattatt atctgactgg
      361 caataaaccg aatgccactg taatggtcga tgttgatcgt cagggctttg ttgatttact
      421 ggctgatcgt ctgaaatttt acgcttaaca cacgtcattg attaagatca ccacgcgtta
      481 cctgggtggt cttaatctgt ttatctcttt ttctttcaag atataaatgc aatggataat
      541 gcagaactcg ccattggtat cgatctcggc actaccaata gtttaattgc cgtctggaaa
      601 gacggtgccg cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt
      661 agcatggatg aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg
      721 catccggata aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg
      781 ttaggcagcg acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa
      841 gaagatgccg aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct
      901 tatttcagcg atgaacaacg caagcatacc cgtttagcag cggagttagc cgggttaaat
      961 gcggtacgct taattaatga acccacagca gctgcgatgg catatggcct gcatacgcaa
     1021 caaaataccc gctcgctggt ttttgatctc ggtggcggca cgtttgacgt cacggtgctc
     1081 gaatacgcca cgccggtgat tgaagttcac gcctccgctg gcgataactt cctcggtggc
     1141 gaagatttta cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc
     1201 acgcttaacg agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc
     1261 aatcaatcgc cattgcacat tcgctggcag taccaggatg aaacgcggga gtgcgaattt
     1321 tacgagaacg aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt
     1381 gaacaggcgt tgcgcgatgc acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt
     1441 ggcggcgcgt cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta
     1501 ccgtatcaaa gttacgaccc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc
     1561 tgccgcttat gcagtgaaga tattgaagag gtgatcctca ctgatatttg cccttactcg
     1621 ttgggcgttg aagttaaccg ccagggcgtt tccggcattt tctcgccaat tattgaacga
     1681 aacaccacag tacccgtgtc gcgtgtagaa acttactcga ccatgcaccc ggaacaggat
     1741 tcaattacgg ttaacgtcta tcagggagaa aaccacaaag ttaaaaataa cattctggtt
     1801 gaatccttcg atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt
     1861 agttatgata tcaacggctt gcttgaagtt gacgtgcttc tggaagatgg cagcgttaag
     1921 tccagagtca ttaatcatag cccggtaaca ttgagcgcgc agcagattga agagagtcgg
     1981 acgcggttat ccgcattgaa aatttatccg cgcgatatgc tcatcaaccg cacctttaag
     2041 gccaaactgg aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg
     2101 gtgatcaccg attttgatgc ggcgttgcag tcaaacgata tggcccgcgt cgatgaggtc
     2161 aggcggcggg cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg
     2221 gcgtaaatgc tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg
     2281 attttgccga gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta
     2341 atgctctgtg gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca
     2401 tcgacatctt catcctccgg ctcgagtttt actttcgtca acagcatccg ggtgataatg
     2461 gcctctaatg gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct
     2521 tgtttcggaa acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt
     2581 gaatcttctt ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg
     2641 aagaataacg actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt
     2701 tccgcccata acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct
     2761 tgcgcatcta ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg
     2821 caatgactct tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactttcc
     2881 cggtacgcgc agagatcggc aagcaggctt tccccctcgc cgcagtacaa cctttgagca
     2941 cacaagtaat atcgggcatc ttcgctttct ggttcttcca tttgccactg tttggctaca
     3001 ggaatgagtt ccgccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt
     3061 aactttgcat catcaggaat gaaccacgga ccttccatcg ccaaaaacgc agtgacgtag
     3121 tcaggatggt aacggcagca acgttccagc gcgaagtaat actcaacagt ctggttctgc
     3181 aacgcaacgg gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc
     3241 tcaagaaaag cctcactttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag
     3301 ttcaaacgct ctgccagcaa agaagcacaa tttaatgaaa gatatcgcgc ttctatagcg
     3361 attgcacaca gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc
     3421 tgaataaatt tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc
     3481 ctgaatgcac gcagtatttc atgttcagcg gcggcatctt tttcttcatc atctgcttcc
     3541 tcgacaggat ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac
     3601 ccctgcggat cggtttcggg atgacataac ggtaagcgag caagataagc ctggcggata
     3661 atgtctattt gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc
     3721 tcacatcctt aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg
     3781 tggcatagaa acgatcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat
     3841 cgtatttgta cagggttagc gcctcgcgta gcgaagtgtt gtcttcgcac tgagaagatt
     3901 gccagtagag acgtcctaac gaataggcgg aaaaatagtg catccagctg tgatagtaat
     3961 ggtgagctcg tacatagacg cgcgattgca gccataaact atcttcttcc gtcagccagt
     4021 tgttgagcac accgacccgt gacagaaacc ccattcgaac ataatcccag gctttgattc
     4081 ccccttctcc acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct
     4141 ccgagccacc agcaattaat gctttccact cttcagggga agagcgatgc catgtcagat
     4201 ataagccagc taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta
     4261 aatcatcacg attatttatt ccccaggatg atgagagatc gaattctttt ataccggtat
     4321 acatttttgg cgagtcatgc gcgggtttgc aaccctgatt aagtatttcc atgggcgcag
     4381 aaagcgcaaa aagaagatat tgttcttcct tattcatttg ttaataatcc cttaagtgca
     4441 ttaactgact actttttatt attgatgagt ataaacttga cgctttcaaa ataaaaagaa
     4501 aatcgaagta ttcacatatg aataaaaaat taatgtatat attcgcaata tttatagttg
     4561 cagcaattac ctgtattagc caacccaaga aaacaacgtt gcgtgataaa gccatggtga
     4621 attatgcctt tgattattta agctcaccag gcagtctacc gttaaccacg gcagccacgg
     4681 agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa ttttatcttc
     4741 atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag
     4801 cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg aaaggcctgg
     4861 gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt
     4921 atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt attaatgtta
     4981 accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg
     5041 aaagattact tgccgggctt cccgcacatt aacgtcgact gtcaaccgaa cagatggagt
     5101 attttcccta tcaaaccagc aaagataaaa atatagaaaa taattttaat gatgcccaga
     5161 cagcctggcc caccatcgtc aacgggcatt ggctgcggtt gcggctccgg ttcaacagtt
     5221 tgcttctcgg taaaattcgc cgcctgaatt aataatcgac gaataattaa tgcttccagt
     5281 ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc ttcggttggt
     5341 tggttcgcct gcactaattg tttggcaact aaatataatg gccataaggg atgttctgta
     5401 tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc gaccagtgaa
     5461 cgccccagag cagagagttt ttcatcgctg agtatttcgt ataaatgaaa acgcgtacgg
     5521 gcgctgtcgc cggggatata aagtagcggt tttccctgat cgttaaccag ttcacgagct
     5581 tcaatcatca tcaccactaa cgggtaataa tcgacccgat gctgacggca ccattgcaat
     5641 attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtcacaaag ttctgccagc
     5701 aagctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg cgcatcttca
     5761 ttatcagatt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg aattcccagg
     5821 cgacctgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc ggggataatc
     5881 actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga ccaaatccga
     5941 gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag atgcagaatg
     6001 gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc cagttcttct
     6061 tcgtctattt ccccgacgtt ttcctcctgc aaccaccaca atctttctgc taataacacc
     6121 acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga ccagcgtaat
     6181 tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg ccaggcggag
     6241 ggcagaaaac gttcgctatc agaggcaagt aacgcacgaa aggcgagcag aatttcatgt
     6301 tctgctacct catattcttc tggttgccaa acagatttag caggcgactg cgcaatccgt
     6361 agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt ttctggatga
     6421 aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt cgtttcctct
     6481 atttcgagaa tcttccagca atttttcata ggtcgctcat atccttaagc gtttccgggc
     6541 atgcagaata gtaagccaat ggttgccagg acaaggtagg gtaatacgca tcatcactgg
     6601 caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc gctaagaatt
     6661 cgcgtaataa ctgcagatga tcatcttcgg gagactgcca atattgccga ccaaaggtat
     6721 aaccggcaaa gtattgccgc cagttacgat aataacgtag agcccgtaga tgaatgcgcg
     6781 actggatcca tagactttct tcttcgctca accagttatt aagtacgccc attcggctta
     6841 aaaaacccat ccggacataa tcccaggctt taattcctcc ctcaccacag cattcggcgg
     6901 tgctggcgac aaattgcgca taagcctgac cttgctcatt tagttcagca agatagtcac
     6961 gccattcaca tggcgaataa cgaaaccagc ggtgataaaa ccctgccaac cgagcggcat
     7021 gaccgttatc gatcattcgg tgaattgtgc gcagcaggtc atcacgggca tcaattcccc
     7081 aggaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggtgat gaatgcgaag
     7141 gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat attttttgcg
     7201 actccatgtc catataattt atccttaaat attcattaaa tgaatattag ccgcattgcg
     7261 aggctcgttt atttatactt ttctgacgcg acataatacg cataaaataa ggcttacgca
     7321 aaaatggaac acataaaaat ataatatgtt ttatctgcaa ccgtaaggaa atgataatga
     7381 ttttcacgtc aagttgctgc gataatttat caatagatga gattatcgaa cgtgctgaaa
     7441 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
     7501 attctccgga cgacgaaaaa gccttttact ggctgaagct ggccgctgag caaggtcatt
     7561 gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt cataaagata
     7621 atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
     7681 acgcccttgg ctggacactg gatcgtggag aagcccccaa ttataaagaa gcggttgtct
     7741 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt
     7801 acagaaatgg caacggagtc gcaaaagact atgcgctggc atttttctgg tacaaacaag
     7861 ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa
     7921 aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc gcacagcagg
     7981 gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
     8041 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa agcgtcggcg
     8101 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
     8161 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
     8221 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
     8281 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
     8341 aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt
     8401 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga
     8461 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
     8521 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
     8581 ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat atctgccagc
     8641 tcctgccata agctttcttt aatcacccgt ataaagacgc tgtcagattc ttctttcagg
     8701 ctctcttcat agctcatggc gaactcttcc aggtcacgtc ttacagctcg cgtcagctca
     8761 tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
     8821 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc gaccagttca
     8881 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
     8941 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
     9001 tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
     9061 ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga
     9121 aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag
     9181 tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga
     9241 agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac
     9301 gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc
     9361 tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca
     9421 acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa
     9481 tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc
     9541 gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat aagaagactt
     9601 ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg
     9661 gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca
     9721 aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat cactggccag
     9781 acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct
     9841 tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta
     9901 tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa
     9961 ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg
    10021 aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa
    10081 cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg
    10141 cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc
    10201 tgaacatcaa accagttatc ctggctgctg acggctctga gccagatctt tctcagcaag
    10261 ccctgactga aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt gaccatgaag
    10321 cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga
    10381 actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga
    10441 tggtgacgct ggaagacggt accgtaatgc cgaccccgga cgaccagctg ccggtgatcc
    10501 tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg
    10561 caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta
    10621 tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg
    10681 attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg
    10741 ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg
    10801 tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct
    10861 tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg
    10921 aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt
    10981 ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg
    11041 ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata
    11101 tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct
    11161 ggaaactggt ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc
    11221 tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg
    11281 atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga
    11341 acaaactggc gaaagcacca accgatggcg agcaggatcg cgctctgatg caggaagcgc
    11401 tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc acgctgtggc
    11461 aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag
    11521 cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa
    11581 tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc
    11641 tggtagcaaa atatcttgat ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac
    11701 tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacattgtt
    11761 gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg ataccacgca
    11821 ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag
    11881 ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac
    11941 gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc
    12001 ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga atgcgaccgt
    12061 gttgatcccc ggccgtgata tctacccgat tagtgccaaa gtcttccgtt cgttctttga
    12121 taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta
    12181 cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg
    12241 ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc
    12301 caccacgctg ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat
    12361 gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc
    12421 caggacgctg ttcgtcaggt agctgcggca caaggattcg aagaacacca cactttttcc
    12481 attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt
    12541 gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat
    12601 gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat cgtccgcggt
    12661 aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc gaatcgcagc
    12721 gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgcgcgc
    12781 gcaaaacagc tcaacttaga actggatgac gctgcaaatc aggtgctctg ctactgttat
    12841 gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac
    12901 ggcaaattga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct
    12961 tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag
    13021 caactgcgtc tggaaggcag cgaaccggtt attttgttgc gcacattaca acgtgaactg
    13081 ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat
    13141 aagcatcggg tatggcagaa ccgccggagc atgatgggcg aggcgttaaa tcgcttaagt
    13201 cagacgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa
    13261 gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa
    13321 cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc tgtttggcgg
    13381 cacctttgat ccggtgcact atggtcatct taaacccgtg gaaacgctgg cgaatttgat
    13441 tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga
    13501 agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt
    13561 tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa
    13621 agagtggcgg caggaacaag ggccggacgt gccgctggcg tttattattg gtcaggattc
    13681 attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat
    13741 cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct
    13801 ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta
    13861 tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa
    13921 cggtgaatcg tgtgaggatt tattgccgga accggtactg acttacatta accaacaagg
    13981 cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat
    14041 ggcgaaacgc aagcgaatgt cgatggtctg tacagcggtc atgcgcccac acccctgacc
    14101 gcacgcggta ttgagcaagc gcaaaatctg catacgctgc tacatggtgt ttcctttgat
    14161 ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac
    14221 cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag
    14281 atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat
    14341 gactggcagc atgcaatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg
    14401 gaacgcttta tcgcaaggct tagtgaattt cagcactatc agaatatttt agtcgtcagc
    14461 catcagggtg tactgagtct gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg
    14521 tggcattttc gcgttgacca ggggtgttgg agcaccattg atatcaacca aaaattcgcg
    14581 acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact tttctgtttt
    14641 tttacgggta agccgcaacg accattgaca gacccgggca ggctgatatt ctccgcagcc
    14701 agactttttt cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc
    14761 tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg
    14821 aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat tcacccaggg
    14881 ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa
    14941 aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat
    15001 catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga
    15061 gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat
    15121 tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta
    15181 tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa
    15241 aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat
    15301 gcccttcgag ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg
    15361 catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac
    15421 cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg
    15481 gaagctggat ggtcgcgacg tcagtctact gattggcggg cctgaagggt tgtcgcctgc
    15541 ctgtaaagcg gcggcggagc agagctggtc gctgtcgacg cttaccctcc cccatccgct
    15601 ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc
    15661 ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa
    15721 ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt
    15781 tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt
    15841 tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg tgcctatcgc
    15901 gcccagccgc ggcattatct acgaccgtaa tggtatccct ctggccctca accgcactat
    15961 ctaccagata gaaatgatgc cagagaaagt cgataacgtg cagcaaacgc tggacgcttt
    16021 gcgcagcgtg gttgatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg
    16081 ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac aagtagctcg
    16141 ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta
    16201 ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa
    16261 agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat
    16321 cggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga
    16381 agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca
    16441 agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct
    16501 gctggcgggt agccgcgcag ctgtggttgt caccgatccg cgtacaggtg gggtgctggc
    16561 gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga
    16621 ttattccgcc ttgttgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt
    16681 ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattga gcgccggggt
    16741 gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactgccag gttcggaaaa
    16801 acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga
    16861 agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc
    16921 cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg
    16981 ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca
    17041 gggtgacacc attccggttg gtatcggtca gggttactgg acagcgaccc caatccagat
    17101 gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc atttgctgat
    17161 gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac cgcccgtcgg
    17221 cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg
    17281 ccctaacggt acggcgcata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc
    17341 tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc
    17401 cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt
    17461 ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta cgctgatgcg
    17521 ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc
    17581 agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc
    17641 tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt gctggtttac
    17701 agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga gcgcaaaatc
    17761 ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt
    17821 tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat
    17881 gctttcggtg ccatctctaa aggtgctcaa cgctggctgg acctcggtat tgttcgtttt
    17941 cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc
    18001 gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat atttatgccc
    18061 acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt
    18121 ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg
    18181 gtggcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc
    18241 gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag
    18301 tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag
    18361 tcacagcttg aatttctccc cgaacgccat accgacttta tcttcgcggt actggcggaa
    18421 gagctgggat tagtgggcat tttgattctg ctcgctctct acattctgct gatcatgcgc
    18481 gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta
    18541 atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg
    18601 gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg
    18661 gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg
    18721 taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc
    18781 ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg
    18841 taacggcccc atagttgaaa ttagcggggc ggatccgcgt ttcgaaccac tgaacgcgac
    18901 ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg
    18961 atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc
    19021 ctctggcgaa gctttcgatc cgacacagct gacggcggcc catccaacgc ttccgatccc
    19081 cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga
    19141 tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct
    19201 taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc
    19261 gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgccagc
    19321 acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat
    19381 tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag
    19441 tagcggtttc ctcggcgctc caacgacctt agcgcctggt gtactggaag gcagcgaacc
    19501 gacgcctgcg ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc
    19561 aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg
    19621 ggccgttagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg
    19681 cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gcccattcgc
    19741 cagcaaagcc gaagccagca ccttgcagca acgtttgcaa accgaagccc aattacagtc
    19801 atttattacc accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg
    19861 acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg
    19921 gcactttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    19981 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    20041 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    20101 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    20161 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    20221 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    20281 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    20341 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    20401 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    20461 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    20521 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    20581 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    20641 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    20701 acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    20761 cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    20821 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    20881 aaccggtttg gtttggtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    20941 tgaccattcc acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    21001 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    21061 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    21121 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    21181 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    21241 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    21301 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    21361 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    21421 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    21481 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    21541 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    21601 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    21661 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg
    21721 aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc
    21781 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    21841 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga
    21901 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    21961 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    22021 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac
    22081 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    22141 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    22201 aagcgacgac taataatatt gttccacgtt tactggaaaa tattttagcg ctactaaaca
    22261 atccggactt cgaatatatt accgcttaat tccatacctc aatggcccaa tttacactgg
    22321 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    22381 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    22441 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    22501 taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg gatagtaata
    22561 atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa
    22621 ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag
    22681 ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc
    22741 agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc
    22801 ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag tccattcttg
    22861 gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc
    22921 cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact cactatccgc
    22981 agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa
    23041 ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt
    23101 tcaccgacaa tatggtgtta atttgccgcg agggaaatcc actactctct ttagaagatg
    23161 acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaagag caaaatttca
    23221 gcggtctgcg gcagagagtt caagaaatgt ttccggaccg gcaaatcaat ctcaccagct
    23281 acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca
    23341 gccgttttta taacctgttt agccgctgct ggccgctgga aaaactgcct tttccgtcct
    23401 taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg cgtgatccca
    23461 tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca
    23521 gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc
    23581 gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag
    23641 ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac
    23701 acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat
    23761 aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag
    23821 ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc
    23881 gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg
    23941 gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt
    24001 tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg
    24061 aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac
    24121 cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg attgcattac tgccattcgg
    24181 gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgtgg tcgtatggat
    24241 cgtgctctgg atattctgac tgcaacgcca ccagatgtgt tcaaccataa cctggaaaac
    24301 gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt acaactggtc gctgaagctg
    24361 ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtggga
    24421 ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg
    24481 acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt
    24541 tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc
    24601 catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaaggg
    24661 atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt
    24721 tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
    24781 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
    24841 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
    24901 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactact agcagtttgg
    24961 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
    25021 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
    25081 gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt
    25141 gtttaagacg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
    25201 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
    25261 gctttgaccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
    25321 cagcgcacga gcggcaagca acgttgacca atgatgctct ttgagcggcc cgcgaaccca
    25381 ggcggcagga agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg ccagctctgg
    25441 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccctccacct cgagtaacgg
    25501 agcgatttca ttaccagcat caacacggcg tgattcctga atggcaaatg catcatagag
    25561 atgcaatttg gcataacggg cgacgatgtt tcctgcctgt agcgccacca gcatattcca
    25621 tgcgcgcccc ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca tgttacgttt
    25681 actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga
    25741 tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacga acagcgatac
    25801 gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac
    25861 agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc acctcctgtc agagtcgtaa
    25921 aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg
    25981 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtgg
    26041 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc
    26101 tggcagcaaa cctgattggg acattcatca taggaatggg attcgcctgg ttcagcagga
    26161 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
    26221 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
    26281 gggcgttact gaacgttttc gtcaaccttc tggggtcttt tgccatgaca gcactggctt
    26341 tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaaacccg ctgattaagc
    26401 gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct
    26461 ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca
    26521 ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg
    26581 aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa
    26641 attaccttta catgaaaaat agacacaaat gctgtgtcgg ttaccagtac accaattgtg
    26701 gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt
    26761 tacttatttt agctattgat tttaaagaag ttactaaaac ttcatttgtc tcaaaactga
    26821 aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc
    26881 tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg gagagggatg
    26941 taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa atgaaaattt
    27001 tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg
    27061 ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa
    27121 cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg
    27181 tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttcaggc
    27241 tgttgccagg tttgtgcaat attttctcta aacgttgtca tccactcatc tgcgttagca
    27301 aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc gacatattta
    27361 ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc atagcaaagc
    27421 tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag ctgctctatt
    27481 tatttccgct tgttcaagtt aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt
    27541 ctgcgatggg aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa aatgcaatca
    27601 aggaaaaatt attttatttt taactagata caacaatcat cttaacgaag tatataatat
    27661 aaatcgtcaa cctatgctca tgttagatta ttttttgagc gcacagccgc agctaacacc
    27721 attgcaatta acaaatttgc atcaatccac catcaattta cacacattat tatgtgataa
    27781 ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac ttcaatgtct
    27841 taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg gcatattttt
    27901 tccttaagct ttaggaattt tttattattt actttggggc ctggagacag gaaaaattat
    27961 gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag ctcgctacat
    28021 cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat
    28081 cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc
    28141 cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat
    28201 gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg cgaatgatat
    28261 ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat
    28321 tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa ccggtctggg
    28381 tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca gtcgtggcgc
    28441 agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt
    28501 ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct
    28561 gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta
    28621 tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac
    28681 tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga
    28741 cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc
    28801 ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt
    28861 ggcttatcgc ggaatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg
    28921 ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac
    28981 ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc
    29041 ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa
    29101 actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc
    29161 aaacctgggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg ccgggatggc
    29221 gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg ttggtttggt
    29281 gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacaggcaa
    29341 gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat
    29401 atgcgttgcg gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg
    29461 gcgcaatatt caagataacg cctggcagtg gtgcgactga tggtcagtgc ctgcgccacc
    29521 gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc
    29581 aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata ggcattaaac
    29641 atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg
    29701 cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat gagataatca
    29761 aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc agtggtaaac
    29821 accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat ccctctgccg
    29881 tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat catcattcgg
    29941 gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg acgaatatat
    30001 tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt taatggagct
    30061 gtcattaggt ttcactttcg gaatatagat tgaaaacaag gtaccgcagg gatcattatc
    30121 ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt acaacccaat
    30181 gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata ttttgtctcg
    30241 tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat catcgccttc
    30301 atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc tggcttcgaa
    30361 ggcgttatca agtaaattgc ccacaatggc tgcaaactcg gtgctatcca gtcctggcgg
    30421 cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt cccgggcgcg
    30481 ctgcacttta ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct cacgcaggct
    30541 gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca cgcgatcata
    30601 ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct cgtgtcgcaa
    30661 tgtacgaagg ctctcgacgt attgttttat ttgcgtcagt tgcgcattga gggtggaaat
    30721 ttcgtcttta ctacgaaagc tgataatggc ccccagcaaa tcatcacctg aacgaatagc
    30781 ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accacatcct gacgtttttc
    30841 atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg gtttacccaa
    30901 ccattgccgt ccgggagagc tcagccccag catctttctt gcgttacgat tgatggcggt
    30961 aatgtaacca tgcggatcca ccgcaatcag cccttcataa accgaactaa acagcgcctc
    31021 ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcattt gccgacggat
    31081 atgcgctgcc aggaaccacg acagcaacat cagaatccct aacagcacga caaacacgcc
    31141 tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca ggtagccaat
    31201 cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg cgcgcatcgc
    31261 catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca gcgcgccctg
    31321 tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa ggcggatcga
    31381 gtgccggtcc ccaatcacca cataatcaaa atcggtatct ctttgtaatt tgttagcgat
    31441 ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt cattggaggc
    31501 aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat agtcctcaaa
    31561 actggccgta aaatattgcg ccagggctgc aatgacaaat attgagaaca ccagaatcag
    31621 caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt tattctcgtt
    31681 aagctgcaac attgttttcc ttgcgggttg tcccgagcct ctttgacgga tgtaattaaa
    31741 atgttgaatc aaaatacata gatattaatt atgatcgtgg caaattttat tcattaaatc
    31801 aattaaatca cttaaacctg ctggtcattt cgtaattaca ttaattaaaa aacttaaaac
    31861 cattaaataa ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgccgaaa
    31921 tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcacc cccctttctc
    31981 ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac aacattcgtt gcaaatcgat
    32041 aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt tcacccgcgt
    32101 aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt
    32161 gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc
    32221 gtgcggtgga attgccggaa atattattaa atgcgttgct atcagtgaat ccgttcgcgg
    32281 tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg agcggcacag
    32341 cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt
    32401 ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg ccacgcggct
    32461 gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat
    32521 tgtgatgaac gccaatccct tcacgaatgg tcaccgttat ctgattcaac aagctgcggc
    32581 acagtgcgac tggttgcatc tgtttttagt caaagaagat tcttcacgct tcccctatga
    32641 agaccggttg gatctggtgt taaaaggcac cgccgatatt ccacgccgga ctgtgcatcg
    32701 cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag
    32761 cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc
    32821 cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca
    32881 gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcac cgcccatcga
    32941 actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg
    33001 tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct
    33061 gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc gtcaaaagac
    33121 ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt
    33181 gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa
    33241 atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggacgtt
    33301 ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac
    33361 tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg
    33421 ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc
    33481 gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc
    33541 cggctgatgc cctgatgttt gacctcgaag actccgtggc attacgtgaa aaagacaccg
    33601 cccgccgcat ggtttatcac gcgctgcaac atccgctgta tcgcgatatt gaaaccattg
    33661 tgcgtgtcaa cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg
    33721 gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg
    33781 aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc accggcctgc
    33841 tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg
    33901 agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac
    33961 gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag gccgcgcgct
    34021 ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc
    34081 tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg atcaacccgc
    34141 gtcagattga tctgttgcac aacctctacg caccaaccca gaaagaagtg gatcacgccc
    34201 gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga
    34261 acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tctcgtgcag
    34321 aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct
    34381 caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc
    34441 cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc
    34501 aacctggaag aagcgattcg tcgctctggt ttacaggacg gcatgacggt ttccttccat
    34561 cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat cgcgaagatg
    34621 ggctttaaaa acctgaccct ggcgtccagc tccctgagtg attgccatgc gccgttggta
    34681 gaacacattc gccagggcgt ggttacccgc atttatacct ccggcctgcg cggtccactg
    34741 gcggaagaaa tctcccgcgg tctgctggcg gaaccggtgc agatccactc tcacggcggt
    34801 cgtgtgcatc tggtacagag cggcgaactg aatatcgacg tggctttcct cggcgtcccg
    34861 tcctgtgatg aattcggtaa tgccaacggc tacaccggta aagcctgctg cggctccctc
    34921 ggctatgcaa tggttgatgc cgacaacgca aaacaggttg tgatgcttac cgaagaactg
    34981 ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgattt aatcgtcaaa
    35041 gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg ctacccgtat gaccactaac
    35101 ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc aggctacttc
    35161 aaagaaggtt tctccatgca aaccggcacc ggcggcgcat cgctggcggt aacccgtttc
    35221 ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgccctcgg tggtattacc
    35281 gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca aactgctgga tgtacagagc
    35341 tttgacagcc acgctgcgca atcgctggcc cgtaacccca atcacatcga aatcagcgcc
    35401 aaccagtacg ctaactgggg ttcgaaaggt gcatcggttg atcgtctcga cgtggtggta
    35461 ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg ctctgacggc
    35521 gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcctctgc gctttccatc
    35581 atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg tggataacgt gctgacctgc
    35641 atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc agttaacccg
    35701 gcacgtccgg agctggcaga acgtctgcag gaagcgggca ttaaagtggt ttccattgag
    35761 tggctgcgtg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac
    35821 cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt acatcaggtg
    35881 aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc
    35941 gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgtctggct caagcgccat
    36001 cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagtgaggtc
    36061 acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc
    36121 tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg tatgttgagc
    36181 attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct
    36241 ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc
    36301 gactattcac tgccgcctcg cagctgcctg ttgtgcgagc aaagcgcagc cgtctgcgcg
    36361 cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact gctgaacgat
    36421 gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa tcgatgagta
    36481 cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga aaccaggcct
    36541 cgtggatcgg attaactgcg gtgcgcataa agatatggcg ctggaagatt tccaccgcag
    36601 cgcgctggcg attcagggct ggctaccccg tttcattgaa tttggtgcct gtagtgcgga
    36661 aatggcacca gaagcggtac tccacggatt acgcccaatt ggtatggctt gcgaaggtga
    36721 tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt ctttagggct
    36781 gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggtaacgc caacaaccgt
    36841 ttgttctacg gcggcaagtt tctgccgtgg tctgaccgat cgcgaactgc gtaccaataa
    36901 ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg gcgcacgcgg
    36961 tgaagccgaa gcgggttatc cactggtgat caaccacgcc ttgccgcatt acctcactct
    37021 gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc tactgatggc
    37081 gatcaacggc gataccaacg ttgcttcgcg cggtggcgag gggggcctgc gctggctaca
    37141 gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccgccg atctcgatta
    37201 tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg gcagtgccga
    37261 cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact tgataaattt
    37321 gaaaatatta attttcggag aacccgtatg tctttagcaa aagataatat atggaaacta
    37381 ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga cggtatgcca
    37441 ccacaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat gatcctcgag
    37501 ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat tggcagtaat
    37561 tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca aaaacaggcg
    37621 ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt tggcgcattt
    37681 atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct tttcctggtg
    37741 aaattcatgg gcaaacgcac gctgacgttg ggttatgcga ttgtcattat cgacattctg
    37801 ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt tccggtcatt
    37861 aaaaacctgc cgccgttgtt taaatcattc cccaacgatc cgtccgcgcg tcgtattggc
    37921 ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat gtttgtcacc
    37981 ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg tatccagatt
    38041 agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct tatcattgcg
    38101 ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga agtggcaacc
    38161 tgggcgggtg atgaactgaa aaccatgggt gcgctgacac gcagagagtg gacactgatt
    38221 ggccttgtat tgctcagctt aggtttgtgg gtttttggca gtgaagtcat taatgctact
    38281 gcagttggtc tgctggcagt ttcgctaatg ctggccctgc acgttgtgcc ttggaaagat
    38341 attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct ggttgtgatg
    38401 gctaacagcc tgactcgttc tggctttatt gactggttcg ccggtaccat gagtacgcac
    38461 ctggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt ctactttgca
    38521 cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt tattctggcc
    38581 gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct ggtgctgtct
    38641 atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat tatttacggc
    38701 tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg ggtgatttac
    38761 atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta atacgttcgc
    38821 caaacgctga ataaatcatc acgcccgcca ggtaagccca cctggcgggc ttttttatga
    38881 tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa gcattctggc
    38941 gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac gccttagcgt
    39001 tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc tggcaaaccg
    39061 gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt cgcctgcaaa
    39121 ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca ggattgccta
    39181 aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt tgcgctactc
    39241 gcccaatccc gaatctacca gaagcgcacg ccagccggat gtgttcatcg ccggaaaccg
    39301 gattatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct ggcggacgtt
    39361 cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt gatccggacg
    39421 catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa gccggaaaat
    39481 tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc gcctttgcca
    39541 aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt aaccctgcgt
    39601 atttgactga aattcagatc tcgatcaaag ctgacgccat caacgctccg ctttctgcaa
    39661 actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg attgataaag
    39721 tgggttatta atcatctgat ttacaaaaca cgctaattcg tcttccataa accgtgacga
    39781 ttaagaatta tacataatgc gctgcattga ggaattccct gaatgtgacg caaatcactt
    39841 caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc ctgacggtga
    39901 gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca tggagtaaaa
    39961 atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga tattctgctg
    40021 gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga gttggatcgc
    40081 gcccaaatgt gttcgccaga agagatgcca cacgacgtgg tgacaatgaa cagccgggtt
    40141 aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta tccggcaaaa
    40201 atgaccgata gcaatactca gctttccgtt atggctccgg taggtgccgc actgctgggg
    40261 ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac ccaccttgaa
    40321 gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta atcccatctg
    40381 cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttac gcaaaatcag
    40441 caatttttcc tggttctcac tgttcacttt tgctgcaaaa ttattccgtt tcgtttgtgg
    40501 gaataaccat ggcagaagca ttttacatcc tgataggatt tctgattatg gcggcgatta
    40561 tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg atcatattgt tccccccggc
    40621 atcgcattca ccagacctga aaccgccttc tgcgccgcct ctgcgctttg tgcaccgggt
    40681 accagaatca ccttacggca ctcctcttca cgtttttcga aaatctcata tccccgggcg
    40741 gcctcttcaa acggcatata gtgggtaaca atttcttctg gtttcagtaa tcctttctca
    40801 attaacggta ataattctcc cagccatgcg tgaacgtggg tctgtcccat tttaaacgtc
    40861 aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc gtagacgccc
    40921 ggtacgctaa caatgccgcc acgcctgacc gccgcaatac actggcgcaa cgctttaccg
    40981 ctgctgccct ccagtttcag gttagtcagc accgtttccg tggtgctgcc tttcgcttca
    41041 aaaccgacgg cgtctattac tgcatccacg ccccggtgac ctgccgtttg ttcaataatt
    41101 gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta gcggtcagcg
    41161 gcgaaacgca agcggtaggg atgatgatca acaacaaaaa tctgttccgc accgagcaac
    41221 cgtgcacagg cgattgtcaa caatcccact ggaccggcac catagactgc aacgcttgaa
    41281 ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat atcagaaagg
    41341 aaaagggctt tatcatctga aagcaaaggc ggtactttaa acggccccac attcccttta
    41401 gggacgcgga catattccgc ctgtccacca ggaacgccac catacaggtg actataacca
    41461 aacaatgccg cgggagctgg tatctgtttt ttattgagcg cagcgccttt acccgcattg
    41521 gtattttcgc aggcggcata ttgctgcatt cgacagaaaa aacagtcgcc acaagcaatg
    41581 acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt tccggtttca
    41641 acgacttccc ccataaattc atgaccaaaa atatcgccat gtttaacctg gggtattttg
    41701 cctcgataaa gatggaggtc agagccacag atcgccgttg ccgtaatacg cagaataata
    41761 tcatctgcct gttcaatgcc cggatcggga acattttcta cctgaacgtg atgtgggccg
    41821 tgataagtca atgctttcat gcaacctcca gagacagttc aaaaaagagt cagtctttaa
    41881 gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa ttcttggtgt
    41941 tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca gacaaatatc
    42001 agggataaat attataatca tggcgaatga tttgttcatg attaacaggg agaaagggtt
    42061 atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag cgacaaagct
    42121 gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta
    42181 cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg ttttgaagag
    42241 catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt caccatcgat
    42301 ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt caatgagttg
    42361 gcagaagaac tgggggctga tgttgtagtt attggttctc gcaacccatc gatttcgacc
    42421 catctgttag gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc ggtgctggtt
    42481 gtgcgttaat tgccagaata aatatcccgc cctgctcgga gccatcccgg cagggctttt
    42541 taatgccata aaaaagccgc caggtttgac cccggcggct aagcaattgc aggtgaatct
    42601 tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt
    42661 ggcaccttcg ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc
    42721 tgcgaatacg cctttgacgt tggtttcgca tttcgcatca atgataatct cacccatgcg
    42781 gttacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctggacgaa
    42841 aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat attccagacc
    42901 aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac
    42961 gtttttcagg ctgcgcagtt tgtcctgcag cacctggtcg gctttcattt ctggcgcaaa
    43021 ttccagcaga gttacgtgct caacgatacc cgccaggtcg attgccgctt ccacgccgga
    43081 gttaccgccg ccgataaccg ctacgcgttt gcctttaaac agcgggccgt cgcagtgcgg
    43141 gcagtaggtc acgcctttgg tgcgatactg atcttcgccc ggaacgttca tgttgcgcca
    43201 ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc
    43261 aatctggtgt agaccacctt caaccgctgc cgggatcagt ttgctggcgc tctggctgtc
    43321 gatcacatca acgtcgtatt catcaacgtg aactttcagt gcgcctgcca gcttctgccc
    43381 ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc
    43441 aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa ttgctgccgc
    43501 tgcacccgcc gggccggaac cgacgattaa tacgtcataa gcatcacgct tgttcagctc
    43561 ttctgccgca cgtttttccg cgccagtatc aattttggca acgatttcag tcaacgtcat
    43621 gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg
    43681 atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt
    43741 cagtacgctc atcaggttca gcgcctgcac cacgtccggg cagttgtggc aagagagcga
    43801 gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg
    43861 cgcttctttc gacggatgac caccggtcca cagcaacgcc agtaccagcg aggtgaactc
    43921 gtggcccagc ggggagcctg caaaacgtgg cccctggttg gaacctgggt tggtgatcag
    43981 gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt tgtctgacag
    44041 ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt
    44101 ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat
    44161 atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc
    44221 ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc
    44281 gattaattac gcgattgcgg aacttagatt ttaccaacca ggtccagaga cggagccaga
    44341 gttgcttcac cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac
    44401 tgtgctgctt tgattttacg cagcaggtca gacgcgtcac ggccaatgcc ttcagcggta
    44461 acttcgattg cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga
    44521 ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc
    44581 atcgcatatt tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag
    44641 tgagtatcgg tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg
    44701 tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag
    44761 aagacgctcc agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg
    44821 aatgcctggt ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc
    44881 gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt
    44941 atcaaatcga taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg
    45001 ccagcggcga taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc
    45061 ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg
    45121 acatggatta caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat
    45181 gacagtgata ggttatgcct tttactcgac ttttgcactg actgaaaagg acaaattaat
    45241 gttaaaaaag atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc
    45301 ttccccagta aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc
    45361 cggaggaatg aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac
    45421 tcccgatggt aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag
    45481 taacacactt atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat
    45541 ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtgtt
    45601 cgccgatccg ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga
    45661 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
    45721 gacagcagcg gcaattcttg cctccaaaga ttccgcaaaa acctggcaag aatatgaatc
    45781 ctctggtggc aagcttaagc taaacgtgcc tgcaaacgta agtacagaac aaatgaaagt
    45841 gttaagcgcc aacgagaaac tcatggacga tctgggggca aatgtcacac cagcgatcta
    45901 ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct
    45961 taatatcatt atggggaata aataaaagat aattcatgga tggttgaaat acatccctgc
    46021 ttttcaacca tcctaaaaaa gcatgaattg ctcgctctgc cctaagtggc tatgctattt
    46081 tagaaaaact taatcctgag ttaagcaaaa aaatataatc aataaaaata tatacgcaaa
    46141 aaacattgat taagtgaata taccatggaa gaaaaatata accggagtag tgtatggcca
    46201 atctctacga cttgaaaaag ttcgacttaa accttcttgt catatttgag tgtatttacc
    46261 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
    46321 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
    46381 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
    46441 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattattt
    46501 atggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
    46561 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
    46621 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttcgt
    46681 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
    46741 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatatc
    46801 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
    46861 aatttcattt cgtggttcct ctttgctgac aatcattaat agcattgcag ttacagattt
    46921 actgggaata gtcccctatg aactttataa ttcctaccgt gactttctta atttaaaaga
    46981 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
    47041 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
    47101 aactacagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
    47161 cactctgctt tgcagtgttt actttataat taacagactt atctcactga tcaccctgta
    47221 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
    47281 gaagctgcac gagagcgtat tacctggaca ctcaacaccc taccccgcgt ttgtgtctca
    47341 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
    47401 atgggcaaaa aaatctgcgt tttgtttatc gactgggagg cgcaattctc ttgcaccatt
    47461 aactatgttc agtccctgcg cgagttgtac accgatgtca tcgaagagtt ttactgggtt
    47521 gcgctcccgc ttacgacgca aaattccctt tcacaattcc aacccgaatg gcagtgctgg
    47581 gaacctgatg tcgaatgggt gcgtcagccc ccgcaagatg cgataaccga tcctgacttt
    47641 ttctgctttt accagcctgg catgaccttc gaacaatttg tacgtgagtt tgccgaatgg
    47701 ttttcacaaa aacgtccggc ggcgatgatg atcggcatcc gtgcggatga gtcctacaac
    47761 cgttttgtcg ccatcgccag tttaaataaa caacgttttg ccgacgataa accatggact
    47821 acggccgcac caggcggtca tagctggtac atttacccca tttacgactg gaaagtggct
    47881 gatatctgga cctggtatgc aaatcatcaa agtctctgca acccactgta taacctaatg
    47941 tatcaggcag gcgttcctct gcgtcatatg cgaatttgcg aaccttttgg cccggagcaa
    48001 cgacaaggat tatggctcta tcacgttatc gaaccggatc gctgggcagc tatgtgcgca
    48061 cgagtcagcg gggtaaaaag tggcggcatt tacgccggac atgacaatca tttctatggg
    48121 catcggaaaa tcctcaagcc agaacattta gactggcaag aatatgcatt attgctgctc
    48181 aatagcatgc cggaaaaaac agctgagcat taccgcaata aaatcgccat ttatttgcac
    48241 tggtatcaga aaaaaggcat cgaggttcca caaacccagc aaggggacat tggcgcgaaa
    48301 gatatcccct cctggcggcg gatatgcaaa gttttactca ataacgatta ctggtgtcga
    48361 gcattatcat ttagtcctac gaaagcgaag aactatcagc gttataacga acggataaaa
    48421 ggaaaacgtc aggaatgggg gatactatgc aacaacgatt aacgcaggat ttaacgcaat
    48481 ttctcgccag cttgccggaa gatgatcgca tcaaagcgat taatgaaatc cgcatggcga
    48541 tccatcaggt tagccccttt cgtgaagagc cagtggattg cgttctgtgg gttaaaaaca
    48601 gccaacttat gcctaatgat tacaacccaa acaacgtggc accacccgaa aaaaaactgc
    48661 tgaaaaaatc cattgaaata gatggtttta cccaacccat tgtggtcacg catacggata
    48721 aaaatgccct ggaaatcgtg gatggttttc accgccatga aattggtaaa ggctcatcat
    48781 cattaaaatt acgcttaaaa ggttatttgc cggtgacctg tctggaaggt acgcgtaatc
    48841 aacgcatcgc cgcgactatt cgtcataatc gcgctcgtgg acgccatcaa atcaccgcca
    48901 tgtcggaaat cgtccgtgaa cttagccagt tgggatggga cgataataaa attggcaaag
    48961 agctgggaat ggacagcgac gaagtgctgc gcctgaagca aattaacggc ctgcaagagt
    49021 tgtttgccga ccgtcaatat tcccgcgcct ggacggttaa atagctaaag ctggcgcagg
    49081 cgttcagctg ctgccagcaa cgtcgattcc ttcttggcaa aacagagacg aatcagttta
    49141 tgtgggaagg gatcggcgca aaacaccgac agcggaatcg ccgctacgcc gtgctcctgc
    49201 gtcagccact ggcaaaactc aacatcatcc agggtagaaa ccgcgctgta atccaccagc
    49261 aaaaagtatg taccttcaca cggtaaaatc tccagccggc tttcatttaa agcattcacc
    49321 agaatatcgc gcttctggcg ataaaagtcc ggtaacgcaa gataatgctc aggttctgca
    49381 cgtagcatat cagcaagcgc cagctgtgcc ggggtattca ccgaaaaggt cagatactga
    49441 tgtaccttgc gaatttcggc gctgatgggc gctggcgcaa cacaataacc cactttccag
    49501 ccggtcatat gataggtctt gccaaatgaa gaaaccgcca ctgcccgctc acgcagctgc
    49561 ggatgcgcca gcacactggc atggccctgt tgtgaaaagt tgatgtgctc gtagacttca
    49621 tcgctaatga caaaaatctc gtgcccggcg atcgcctgcc acaaagcggc gaaatcagcc
    49681 tgctgccaga tagttgcact ggggttatgc ggagtgttga ggatcaccag tctggtgcgc
    49741 tcgcttaata atgcggcaaa ttcctgccag tcaacgcgaa aatgcggtgg ttgcagtgcc
    49801 atacgcttca ctattccccc agaaagcgcg atggcggggg agtaactgtc atagctggga
    49861 tcaaaacaaa tcacttcatc gccattgcgc accagtgcgg taatcgccgc gtataacgct
    49921 tccgtcgccc ctgccgttac ggtgatatcg ctatcggcat ctggttgata gccatacaaa
    49981 cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat gggcgcgtat
    50041 tggtttgccc cctgggcaac gtggtacgcc agccgctcct gtaaatagtg cggaccatca
    50101 aaatcaggaa agccttgcga caggttaatc gcctggtgtt gctgcgccag cgcgctcatc
    50161 tgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag agggttattt
    50221 gtcattttta ttgtacctga cgaagttgcg tgttgaaggc actataacac gatgctagta
    50281 tttggcaatc aagacgttta gatgtctaaa taaaacaata aggacaacac aacatgcctc
    50341 acaatcctat ccgcgtggtc gtcggcccgg ctaactactt ttcacatcca ggaagtttca
    50401 atcacctgaa cgattttttc actgatgaac aactttctcg cgcggtgtgg atctacggca
    50461 aacgtgccat tgctgcggcg caaaccaaac ttccgccagc gtttggactt ccaggggcaa
    50521 agcatatttt gtttcgtggt cattgcagcg aaagcgatgt acagcaactg gcggctgagt
    50581 ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg cgcactgctc gacaccgcga
    50641 aagccctcgc ccgccgtctc ggtctgccat ttgttgccgc tccgacgatc gccgccacct
    50701 gcgcagcctg gacaccgctc tccgtctggt ataacgatgc cggacaggcg ctgcattatg
    50761 agattttcga cgacgccaat tttatggtgc tggtggaacc ggagattatc ctcaatgcac
    50821 cgcaacaata tctgctggcg gggatcggtg acacgctggc gaaatggtat gaagcggtgg
    50881 tgctggctcc gcaaccagaa acgttgccgc taaccgtgcg gctggggatc aataatgcgc
    50941 aggccattcg cgacgtcttg ttaaacagta gcgaacaggc acttgccgat cagcaaaatc
    51001 agcagttaac gcaatcattt tgcgatgtgg tggatgcgat tatcgccggg ggtggaatgg
    51061 tcggtggtct gggcgatcgt tttacgcgtg tggctgcggc ccatgccgtg cataacggtc
    51121 tgactgtcct gccgcaaacc gagaaatttc tccacggcac caaagtcgct tacggaattc
    51181 tggtgcaaag cgccttgctg ggtcaggatg atgtgctggc gcaattaacc ggggcgtatc
    51241 agcgttttca tctgccgact acgctggcgg agctggaagt ggatatcaat aatcaggcgg
    51301 agatcgacaa agtgattgcc cacaccttgc gcccggtgga gtccattcat tacctgccag
    51361 tcacgctgac accagatacg ttgcgtgcag cgttcgaaaa agtggaatcg tttaaagcct
    51421 gacgtgcaaa tttagcagca gcgcgcgccc ccttttccac cgtagcgcgc gtcctgccgc
    51481 tcacggaaaa actcctcgta ggtcatcggc gtttgatcgg gatggttaac ccgcatatgt
    51541 tcgacatagt tgtcgtaatc aggcatacca atcatcagct tcgccgcctg acctaaatat
    51601 tttccggctt ttgccagtga atcaaacatc ttggcctccg ctaaccatgt tgctgaaaga
    51661 gtgccggatg cggcgtgaac gctttatccg gcctacaaat gctatcaacg cactcggtca
    51721 gtcccctcgc cccatcgggg agagggttag ggtgaggggg aaccctcggc actatgccaa
    51781 cattcgccaa catcccccct cactctgact ttagtgcgca cctttcgcct gcgctacgat
    51841 ctcctcaaca ttttccggca ttggctcata cggcgtttct ttcgccgttg gcttcggctc
    51901 tttcaatgcc gcaagtgccg tcttaatcga gaacagtgcc agaaccacca cgaccaccat
    51961 aaagaagatg gttaacccgg catccagacg gttgttgaac accagttgcg ccagctgtga
    52021 ctcagtatac tgcgacggaa tattgccgct gtcgatcatt gcctggaact tgttagcaat
    52081 ggccaggaag ccgactttcg catccgggct aaacgctttc tgccagcctg cggtcagggt
    52141 acaaatcagt agccaggccg ttggtaccag cgccacccag gcgtaacgtt gacgtttcat
    52201 cttgaacaac accacggcac agagcatcag cgccatccct gccagcatct ggttggcaat
    52261 accaaacagc ggccacagag tgttaatacc gcccagcgga tcgaccacgc cctgatggag
    52321 gaagtagccc cacgccagta cgcacagcgc cgttgccagc aggttagcag gcagtgaatc
    52381 ggtccgcttc aggccaggag acaccacgcc cagcagatcc tgcaacataa agcgcgcagc
    52441 acgcgtaccc gcatctaccg ccgtcagaat aaacagagct tcaaacagaa tggcgaagtg
    52501 ataccagaac gccacatcca tcataccacc cagcgcaccg tgcaggatgt aagccatccc
    52561 caccgccaac gtcggcgcac cgcccgcacg ggaaatgatc gactgttcac ccacttcgct
    52621 ggcaatctgg ttgagcgtat ctggtgtaat gctaaagccc cagctactca ccacctgcgc
    52681 ggcagaagcg accacatccg ccgtccctgc cggagccagc accgccattg ggctgttcat
    52741 ggcaaagtaa acgcccggat cgatgataca ggcggaaacc agcgccataa tcgccacgaa
    52801 ggattccatc aacattccac cgtagccgat aaaacaagcc tgcccttcgt tcgccagcat
    52861 cttcggcgtg gtgccggaag agatcagcgc atggaagcca gacaccgcgc cacaggcgat
    52921 ggtgataaac aggaacggga acaggttacc ggtccatacc gggccagtgc catcgacaaa
    52981 tttggtcagc gcaggcatgg tcagcgtcgg gcgcataatc aaaatgccta ccgccagacc
    53041 gacgatcgtc ccgattttca ggaaggtaga gaggtagtca cgcggggcca gcagcaacca
    53101 caccggcagc accgccgcca caaaaccgta acccaccagc atccaggtca gctgcacgcc
    53161 ggtaaagtca aagtacggtg cccaggtcgg actttctgcc acccagccgc cagagataat
    53221 ggcgaaaatc aggaatacca gaccgatgac cgacacttca ccaatacgcc ccggacgcag
    53281 atagcgcagg tagatcccca taaacagcgc cagcggaatg gtgaacgcaa cggtatacgt
    53341 tccccacggg ctatgagtca gggctttcac cacgatcatc gccagtactg caaggataat
    53401 gaccatgatc ataaagcagg ccaccagcgc tatcaccccg gcggttggcc ccatctcttc
    53461 tttgaccagc tcacccagcg atcgaccgtc acggcgcgta gaaacaaaca gcaccatgaa
    53521 atcctgcacc gcaccggcga gaaccacccc ggcgagcagc cagatcatcc ctggcaggta
    53581 acccatttgc gccgccagta ccggcccaac caacggacct gctccggcaa tggccgcaaa
    53641 atggtgaccg aacagcactt tcttgtccgt cggcacatag tccagcccgt cgttatggcg
    53701 caccgctggc gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacagccc
    53761 ataaaaacgg taagcgatca gatagataca gaccgacgcc accacaatcc acagcgcgtt
    53821 gatctgttcc ccacgattta aagcaatgta tcccagagca aatgctccca ttacagagag
    53881 cactgtccag acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca
    53941 tagatatgtt acattttgtt tctataacat ctttgtacga tttaacaaca cggtaaccat
    54001 gtaaatcgga gttgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac
    54061 cgagttttcc tgcaatctca tttaattctg tcgggctcat cccaaaactg ccgtacccag
    54121 ccgacaagtg cagcaacgcc gcccctgttc atcgaaaacg acgatttccc agctctgatt
    54181 ttgccgccca agatgcagcg gctggcagac gccgcgtacc ttgccttcag acaccgggcg
    54241 atgatgggtg gcgttaagtt ccgtacccac cacacactgc ccgtcacggg tcatcataaa
    54301 tccggccatc gatcccagcg tttcagccag cgccgctgac gcgccgccat gcagcaggcc
    54361 aaacggctga tgagtacggg tatcaaccgg catttcggct tccagcacat catctcccag
    54421 acgggtatac acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagttc
    54481 gtcgagcgtt aaatggcgtt tccagatcat gcttatgccc ccagcgttga gccgccatcg
    54541 accacaatat cctgtagggt gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg
    54601 ttggcgatct cttgtggacg ggcgattttc cccagcggaa tgccgagttt aaactgctcg
    54661 ccaaagccgc gaatacgctg ttcttcggcg tcatcgctca cccacagcgt gcgttgcata
    54721 tcggtgtcgg tggagccagg cgaaaccaca ttacagcgca cgccgctacc cgccagttcc
    54781 agcccgacgc tcaacgccag gcttttcagc gccgctttcg atgcgccata agcactcatg
    54841 ccaatacgcg gcgtgtgcgc ggcgtcggac gccacagtga caatcgcccc gccccgctga
    54901 cggcgaaact ggttcatggt ttgctggaac aggttaaacg caccgccgac gttaaccgca
    54961 aaagtctgct gccagtcctc tttactgagc tgatcggtcg cgcccatgcg caaaattccc
    55021 gccgcattga ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc
    55081 gcgacctgcg cagcatcggc aacatccatc acttcggtcg caaagggata ttgctcctga
    55141 gcgaacgctt gatcaaaacc ggtaactttc gctcccgcct caacaaacgc cagcgccgtg
    55201 gcgtagccga tacctttacc tgcgccggtc acccagacat ttttaccgct gaaatccatt
    55261 atttcacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccagca
    55321 tgacaaagtc gatatcacca tgcactttgc gccagcgcgc cgccagcgcc atcatgcgca
    55381 ccgaatccag accgtagtcg atcaggttat cgtcatcgaa cggttcatcg gactcgtcca
    55441 gcaacggcag gataacctca cgcagcgccg ctttgctggc ggggacaggt gctggcagta
    55501 attcttcagt catcaccacc cggccagacc gcccggccac atatttcagc gacatcaaat
    55561 gctcgtcacg gctgaaatcg gccagcgcat ccgccaccat aaacggttta atatcgcgca
    55621 taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atataccccg gtaataatca
    55681 gctggttacg tccactctct ttgagcattt gctccagcgg agaacgatga aacgcgctgt
    55741 agcgccactt caccagcacc gtgtcgtcgg catctggcgt caggcgatcc accacctttt
    55801 gctgttccgg cgaacgggtc aggcccggcc cccacatatc attcaacagc gcccgatctt
    55861 catcgctctg ctctttcggc tgggcggtgt aataaaccgg aatattgtgc tgtttgcagt
    55921 agtcgcgcag cgcagcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc
    55981 agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa
    56041 aggcccagtc aactttattc ttagggatat cgtgagactc cggcagtgcg taagcctgta
    56101 attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga
    56161 cgtaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc tacacactcc
    56221 acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc
    56281 accgcgcgca gcggctcttt taccaccaga taagcgcagc ttttttcgcc catcagctca
    56341 tcttccatgc tcaccagtgc ggcgtagatc accgccgggt ggcgcagcag caggttttcg
    56401 atctcttcgg cagcgatctt ctcaccgcca cggttgatct gatctttctc gcgcccctgc
    56461 acggtgatgt acccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg
    56521 gcatcaaagg cactggcatt gtgctgcgga cttttgtaat aaccgcggaa ggtgtacggc
    56581 ccgcgcgtca tcaggcgtcc aacttcccct tgcggcagtg gatttccttc agcatcagca
    56641 acccatactt catcgtccgg acacattggg taaccctggg tatggataat tttctccgcg
    56701 ctatcatcaa gtcgggtgta gttcaccagc ccttccgcca tgccaaacac ctgctgcaac
    56761 aggcagccaa tctcagcggg aatacgcgcc gcaagggtgg ctgaaagacg tgcgccgccg
    56821 acctgtaaca gtttcagcga ggcaagctgc gcccggcttt caccttcggt cagcgcctgc
    56881 aaccacaggc tgactgccgg tggcaccagc gcggtgacgt tcacctgatg tttttcaatc
    56941 aatgggaagc aaagcgtggc gctgggatcg gccgccagaa caagcgttcc tccggcaaga
    57001 aagacgccca gcgatcccgg cgaactcatg gcgtagttat gagccgccgg gatcgcgcac
    57061 aggtagcgtg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag
    57121 tagtcgttat gagtgcgcgg gatcagtttc ggtgtgccgg tggtgccgcc ggaaagctgg
    57181 aaataggcca cttcatcagc aggtgatggc gtggcagtaa aatcctcagc cggatggtta
    57241 atcgcatcct gcaagttatg ctcaccgctg tcgttgtgca gttgcaccac gcgaatggag
    57301 gaatgttctg tgacgaaagt attgaggaaa tcatccccgc taaacagcgc atgttggcga
    57361 tcggcaatca gcaatgcggg ttcaatctgg ctggcatagg cgttcagttc actacgctga
    57421 tggctgaaca gcgccagcac cggcgcaacg cccagtttca gcagcgcgaa aaaggtaata
    57481 tacaattcag cgacgttacc cagttgtacc agcgcggttt caccaggttt aatgccctga
    57541 cggcgtaaac tacacgcgag gttatccgcc gcctgattca gctcccggta actcaactgt
    57601 cgctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc
    57661 agcggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag
    57721 cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta
    57781 gaaagtttga cgcctgtttc gcgccactca cccaacggtg acgacgcagg cacaatcccc
    57841 gctccggcaa acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc
    57901 cattcgccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg
    57961 aatggctcca gttcagcaat aacctgggtc gcggcctgat gcgggaaacc gctcagcgcg
    58021 ggggttggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct
    58081 tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga
    58141 acgtgtaact cactactgcg ttcgcgcagt acctctttca tcgcctgagt caccagttca
    58201 tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact
    58261 tcatccggct gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct
    58321 ttacgtagca gcagttccgg gctggccccc agcaggacgc caccatcagc cagcgggaca
    58381 tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca
    58441 atggcggcgt cagtggtgat atcaatcaat cgtgacaaca ccactttgtc gacctgcggc
    58501 gtggcggtaa gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg ttcaggaatt
    58561 gcctggcgtt ccaccacgtt cagcgactgg ctgcgggtaa aacggcgggc tgaggtctgt
    58621 ttttcctggc gggagaacga ctgccaggat tcgggaatat acagcgacga aggctgacgt
    58681 ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg
    58741 gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc
    58801 ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag
    58861 aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta
    58921 tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg
    58981 gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac
    59041 tcggggttgc atctggttgt ggttacaatg aaaatgagaa gcattattga tggattcgca
    59101 taagcgcaat gtgatgtcct gcgccgttct gccccctctc ccttccaggg tgagggctgg
    59161 ggtgagggtt aatgttcgca ccagtgctgg ctgttcccct caccctaacc ctctccccaa
    59221 aggggcgagg ggacggattg tgcgctttgt cgaatttgtc attacgccct taaccttatt
    59281 aataacagga agctgatttg tgagactcgc cccgctctac cgcaacgccc ttctattaac
    59341 aggacttttg ctttcaggaa tagccgcagt tcaggccgct gactggccgc gtcagattac
    59401 tgacagccgt ggcacacata cactggaaag ccagccgcag cgtattgttt ccaccagcgt
    59461 caccctgacc ggctcactgc tggcgattga tgctccggtg atcgccagcg gcgcgaccac
    59521 gccgaataac cgcgtcgcgg atgaccaggg ctttttacgc cagtggagca aggtggcgaa
    59581 agaacgcaaa ctgcaacggc tctatatcgg cgaaccgagc gccgaagccg ttgccgcgca
    59641 aatgccggat ctgattttaa ttagcgcaac cggcggggat tcggcgctgg cactgtatga
    59701 tcagctttcc accatcgccc cgacattaat catcaattac gacgacaaaa gctggcagtc
    59761 gctgttaacg cagcttggcg aaattaccgg gcatgagaaa caagcggcag agcggattgc
    59821 acagtttgat aagcaactgg cagcggcgaa agagcaaatc aaattaccgc cgcagccggt
    59881 cactgccatt gtctataccg ccgctgcaca cagtgccaat ctctggacgc cggaatcagc
    59941 acaagggcag atgctggaac aactcggctt tacgctggcg aagttgcccg cagggttaaa
    60001 cgccagccaa agccagggca aacgccatga catcattcag cttggtgggg aaaatctggc
    60061 tgctgggtta aatggcgagt cactattcct gttcgccggt gatcagaaag atgccgatgc
    60121 gatttatgcc aatccactgc tcgcgcacct tcccgcagta caaaacaagc aggtttatgc
    60181 gctgggaacc gagacgttcc gtctggatta ctacagcgcc atgcaagtgc tggaacgact
    60241 taaggcgctg ttttaagcat taactgtcag acgctgtcac ctgcggcggc gtctggcgaa
    60301 aacgtcgcaa ctccaccagc accagcaata acaacacgcc gataatcaac aaaccaaaac
    60361 cgctcgcgct tgcggaagca accggtgtca tcatcgcgcc caaaccaccc agcagcgccg
    60421 cgcctatggc atcgcccgtc acgttctgcg ccgtccacaa accgttaatc cgccctaaca
    60481 tcgcttccgg ggtttgcgtt tgcagcattg tatattgcag caacgaacta accgcgctca
    60541 gccagccgaa cagcgccaga caaaccacgc ctaaaatcca catcggcatc aggccaaaca
    60601 gaccaatggc gaggaacgat cccagcgtgg agagcagcat caataaccct ggtcgcgcac
    60661 tatgtgccag cttcccgctg gttaacgcac caatagccgc gccgagcggg atcgccgcgt
    60721 agagaaaacc aatctgtgcc gctgacatct gccagttgtc agccagcgcc ggatacagta
    60781 cccgcaccgc gctcgccatc gtcaataaac cacccagcag cgcaatcccg cccaccagcg
    60841 ggctggcaag cagaaaacga aatcctgcca gtaatgattt caacggatgc tcacgcggct
    60901 gcggtggcgg tggcaacgcc ggaaggctta acaacggtag caaggtaata aacgtgcccg
    60961 ccgccgccag cccgtagttc caggctacgc caccggtcgc cagcaataaa ccgccaatca
    61021 tgggcgaaat caccgacccc agacgcacgg tcaacatggt gatcgccccg gcctgcatta
    61081 agttttcacg ccctaccagt gcaggtgtcg ccgccagtag cgccgtaacg ccaagtgatg
    61141 cgaaaaaacc atcccataaa ccaagtaaat agattgccag caatgacggt tcaggcagca
    61201 gtgcattaag gcacagtcca atgaagccaa tgccacaggt gccgcgcgcc agcaaaatca
    61261 cttttttgcg ttcatagcga tccgccagca caccgccgac catcaggcca acaaacatcg
    61321 cgccgccggt cagcgtcacc gaaagcccca cctgccaggt agaatgtgtc atcatctgga
    61381 tctgcaccgg caccgcgacg ccgagcaaac ccagagaaac aatagagatg aaacgagcga
    61441 ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct gaggttaagc agccaggatt
    61501 gtttattcat tacaatgcct tgccatcaaa tattttaaat acctattctt aggctgcaca
    61561 tgctaacata tccaaataag atcgataacg ataattaatt tcattatcat ggaagttcgt
    61621 atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc ccggattgct gttattactg
    61681 attatcgcga cggcattaag tctgctcatt ggggcaaaat cactccccgc ttccgtagtg
    61741 ctggaggcct tctccggcac ctgccagagc gccgactgca ccatcgtgct cgacgcgcgt
    61801 ctgccgcgta cccttgccgg tttactggca ggcggcgcgc ttggccttgc cggggcgtta
    61861 atgcaaaccc tcacccgtaa cccacttgcc gaccccggct tgcttggcgt gaacgccgga
    61921 gccagctttg ccattgtgct gggtgcggcg ctgtttggtt actcttccgc acaggaacaa
    61981 ctggcgatgg ccttcgccgg ggcgctggtg gcctcgttga ttgttgcctt taccggcagc
    62041 cagggcggcg ggcagttaag tccggtgcgt ttaaccctgg cgggcgtggc gctggcggcg
    62101 gtgctggaag gactgaccag cggcatcgcc ctgcttaatc ctgacgtcta cgatcaactg
    62161 cgtttctggc aagccggttc gctggatatt cgcaatctac ataccttaaa agtggtgctg
    62221 atcccggtgc tgatcgccgg agcaactgcg ctattactga gtcgcgcgct gaacagtttg
    62281 agcctcggca gcgacaccgc gacggcgctg ggcagtcgcg tggcgcgcac acagttgatt
    62341 ggtctgctgg cgattaccgt gctttgtggt agtgcgacgg cagtagttgg cccgattgcc
    62401 tttattggcc tgatgatgcc gcatatggcg cgttggctgg tgggtgccga tcatcgctgg
    62461 tcgctgcccg tcacgctact tgctacccct gccctgctgc tgtttgccga tatcatcggg
    62521 cgggtgattg tgcccggcga actgcgcgtt tctgtggtca gtgcgtttat tggtgcaccg
    62581 gtgctgatct tcctcgtgcg acgtaaaacg cgaggtggtg catgatttac gtctctcgcc
    62641 gattactcat cacctgtttg ctgctggttt ccgcctgtgt ggttgcaggt atctggggat
    62701 tacgcagcgg tgccgtcacg ctggaaacct cgcaggtatt cgccgcgctg atgggcgatg
    62761 cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt accacgcgtg ctgatggcgc
    62821 tgttgattgg cgcagcactg ggcgtcagtg gcgcgatttt tcagtcgctg atgcgtaacc
    62881 cgctcggcag ccctgacgta atgggcttta acaccggggc gtggagcggc gtgctggtgg
    62941 cgatggtgct gtttggtcag gacctgacgg ctatcgcgct gtcagcaatg gtgggcggca
    63001 ttgtcacttc gctgctggtc tggctgctcg cctggcgcaa cggcatcgac acctttcggt
    63061 tgattattat cggtatcggc gttcgcgcca tgctggtggc ctttaatacc tggctgttgc
    63121 tgaaagcgtc tttagaaacg gcgctaacag caggtttgtg gaatgccgga tcgctcaacg
    63181 gcctgacgtg ggcaaaaacc tcgccttccg cacccatcat tatattgatg ctcattgccg
    63241 ccgccttact ggtacgccgg atgcgcttgc tggaaatggg cgatgatacc gcgtgtgcgc
    63301 tgggcgtcag cgtcgaacgt tcgcgtctgt taatgatgct ggttgcagtg gtgcttaccg
    63361 ctgcggcaac agcgcttgcc gggccgattt cctttattgc tttagtcgca ccgcacattg
    63421 cccgacgcat tagcggcacc gctcgctggg ggctaaccca ggcggcgcta tgcggggcgc
    63481 tgttactgct ggcggccgat ctctgcgccc aacaactgtt tatgccgtat caacttccgg
    63541 ttggcgtcgt taccgtcagc ctcggcggta tttaccttat cgtcttgtta attcaggagt
    63601 ctcgcaaaaa atgaccgaat cagtagcccg tttgcgcggc gaacagttaa ccctcggata
    63661 tggcaaatat accgttgcgg aaaatctgac tgtagaaata cctgatggtc acttcacggc
    63721 aattatcggg ccaaatggct gcggtaaatc cacgttactg cgtaccttaa gccgcctgat
    63781 gacgcctgct catgggcatg tctggctgga tggcgagcac attcaacatt acgccagtaa
    63841 agaggttgca cgccggattg gtctgttggc gcaaaatgct accacgccgg gcgatatcac
    63901 cgtgcaggag ctggtggcgc gtggacgtta tccgcatcaa ccgctgttta cccgctggcg
    63961 caaagaggat gaagaagcgg taacgaaagc aatgcaggcc acgggaataa ctcatctggc
    64021 agatcaaagc gtggataccc tttctggcgg acaacgccag cgcgcgtgga tcgcgatggt
    64081 gctggcccag gaaacggcaa ttatgctgct cgacgaaccc acgacctggc tggatatcag
    64141 tcatcagatt gatttgctgg agttgttaag cgaactgaac cgcgaaaaag gctataccct
    64201 ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac gccagccatt tgattgcatt
    64261 gcgggaaggg aaaattgttg ctcagggagc gccgaaggag attgtcactg ctgaactgat
    64321 tgagcgcatt tatggtctgc gctgcatgat cattgacgat ccagttgccg gaacgccgct
    64381 tgtggtgccg ctcggacgaa cggcaccttc aaccgcaaat agttaaacta agtggtctgc
    64441 catcatggca tcctgttttc tggatgccat cgcatagcgc aacagcacgc caccacaagc
    64501 caccatcccg ccgatcaacg cggaaaggat cacaataatc gccttacccg gaccgtcttt
    64561 tttcactggc aatgacgggc ttaactgata tttaaacggc gtaaaattca catcgttgac
    64621 atgtgctttt gttaattgct cgacaagata ctgccgatta cgtaattcac cgttcagttc
    64681 cgcaacgtca gtgaccgctt tttctatttc cagtttgcgt tcaataccgt ctgcaccgag
    64741 agaaatagaa aaatcggggt catctttaac ggcctgacca ttactgtaaa cgggcttttt
    64801 aattcctgcc gcgttggcaa tgtcgagtga ataattgagg cgctgaatgt ttgcatcaag
    64861 ttgatttttc gttttaatgc gatcctgagc cagtttttct ttttcaaact gggttttgat
    64921 ctccagttta ttacggacgt tttctagcga ctctttcacc accaacgtag agatataatc
    64981 gatatacccg ctcaaaacgg tctgcgcctc ttcactggtt ggcgcggtaa aacttagcgt
    65041 ccaggaggta tacagtgacg gttcatcttt tttcttactg gcattgtcat caaccgcttt
    65101 cattttttcg ctcaatgcga caattgcgcg atgcaaatcc agttcgtcga ttttcgcctc
    65161 ttttaattgg tccatcacat aaggtgatga acgcaggtac tcttccagca agctaaccga
    65221 ctgaaacttc ttgataaaca ggttaaatgc ttctgtacga tcaattttga tatccagatc
    65281 cagcacacga agcttagtga atgttttctc caactcttgc cactgaacag gttctggagg
    65341 cgtgacaacc gccgcgctgg tccatttttg cggcaggatg aaagagatca gcaagcctgc
    65401 gcaggcaaac gcaaaaacga ccgccatgac cgtttttttg gcccgccata aaactgagat
    65461 tagattaagt aaatcaattt cattattact gggcgacgcc agaggataat cgggaaaatg
    65521 agcgtcactt ccctgtttaa tattcagtga tgacatgggg tataacctga aaatgagtcc
    65581 aataggggga aatctccacg gcgctcactt taccagttgg tgagaattat ccgaagctga
    65641 agtttgtaaa ttccttccaa tgaatgcaac attcactcta tttaagagaa ataaccagca
    65701 aacttataaa ttaaacaaat gattaaccca taattatttc ctgtaattat gggttaatag
    65761 ataatgttaa tttacctgtt tagcgttgcg cgaataatcg gcccaatttt ttcaaacgcc
    65821 cctggagaga taatatccac atgcgcacaa tcctgacgat agatatccag ctccgctatc
    65881 cacggcgacc aggcgcgttc gggactcata ccttcctgaa gtgtgcgttc agcaacaaac
    65941 agcgtcgctt taccgtcaaa cggtacgcta tgagcagtcg tcagcaggcg cacagcatca
    66001 gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag tacttccctg ctgtgctgcc
    66061 aggaaggcct cgcgttcgcg gttaatctcc gccagcactt ccgggtccag accattagct
    66121 tctttttcct gccagttttg cgtttctggc ggccaggtat ccagcaagcc aagaaatgcc
    66181 acctgttcgc cacgggcacg cagtcgcgcc gcaataccct gcgccagcgt accgccaagg
    66241 gaatacccca gcaggtaata agggccatgc ggttgttgtt caagtaacgt tgccagatgc
    66301 gcttcgcaga cttcatccag gtttgccgcc gtctgcatgg ggccattggg gcgcggtgac
    66361 tgaatgccga taatcgacca ttgtggatcg agataacgcg agagcacgct gaactgccag
    66421 gcaaaaccgg acgcaggatg gaaacaaaac agcgtcgggc cattaccttc acgcaacggc
    66481 agaatggttt cgaatcccat acgccgggtg ctgtcttctt cagcatcaat aatcgttgcc
    66541 agtttggcga cagttgacgc gaccatcact tgc
//
LOCUS       DAHBHC010000018        61945 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHBHC010000018 DAHBHC010000000
VERSION     DAHBHC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.018"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..256)
                     /locus_tag="M2A35_001508"
     CDS             complement(<1..256)
                     /locus_tag="M2A35_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="cell division protein; signal recognition protein
                     receptor; functions in the targeting and insertion of
                     membrane proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCB7197826.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQ"
     gene            406..1002
                     /gene="rsmD"
                     /locus_tag="M2A35_001509"
     CDS             406..1002
                     /gene="rsmD"
                     /locus_tag="M2A35_001509"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCB7197827.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            992..1261
                     /locus_tag="M2A35_001510"
     CDS             992..1261
                     /locus_tag="M2A35_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCB7197828.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(1264..1623)
                     /locus_tag="M2A35_001511"
     CDS             complement(1264..1623)
                     /locus_tag="M2A35_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCB7197829.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            1764..2390
                     /locus_tag="M2A35_001512"
     CDS             1764..2390
                     /locus_tag="M2A35_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCB7197830.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            2464..4662
                     /gene="zntA"
                     /locus_tag="M2A35_001513"
     CDS             2464..4662
                     /gene="zntA"
                     /locus_tag="M2A35_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCB7197831.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(4764..5009)
                     /gene="tusA"
                     /locus_tag="M2A35_001514"
     CDS             complement(4764..5009)
                     /gene="tusA"
                     /locus_tag="M2A35_001514"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCB7197832.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            5230..5895
                     /locus_tag="M2A35_001515"
     CDS             5230..5895
                     /locus_tag="M2A35_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCB7197833.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            5968..6525
                     /gene="dcrB"
                     /locus_tag="M2A35_001516"
     CDS             5968..6525
                     /gene="dcrB"
                     /locus_tag="M2A35_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCB7197834.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(6529..7746)
                     /locus_tag="M2A35_001517"
     CDS             complement(6529..7746)
                     /locus_tag="M2A35_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7197835.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKNIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            7878..8927
                     /locus_tag="M2A35_001518"
     CDS             7878..8927
                     /locus_tag="M2A35_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCB7197836.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8982..9569
                     /gene="acpT"
                     /locus_tag="M2A35_001519"
     CDS             8982..9569
                     /gene="acpT"
                     /locus_tag="M2A35_001519"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCB7197837.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            9680..11254
                     /gene="nikA"
                     /locus_tag="M2A35_001520"
     CDS             9680..11254
                     /gene="nikA"
                     /locus_tag="M2A35_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCB7197838.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            11254..12198
                     /gene="nikB"
                     /locus_tag="M2A35_001521"
     CDS             11254..12198
                     /gene="nikB"
                     /locus_tag="M2A35_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCB7197839.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            12195..13028
                     /gene="nikC"
                     /locus_tag="M2A35_001522"
     CDS             12195..13028
                     /gene="nikC"
                     /locus_tag="M2A35_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCB7197840.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLADAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            13028..13792
                     /gene="nikD"
                     /locus_tag="M2A35_001523"
     CDS             13028..13792
                     /gene="nikD"
                     /locus_tag="M2A35_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCB7197841.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHNMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            13789..14595
                     /gene="nikE"
                     /locus_tag="M2A35_001524"
     CDS             13789..14595
                     /gene="nikE"
                     /locus_tag="M2A35_001524"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCB7197842.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            14601..15002
                     /gene="nikR"
                     /locus_tag="M2A35_001525"
     CDS             14601..15002
                     /gene="nikR"
                     /locus_tag="M2A35_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCB7197843.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            15201..15947
                     /locus_tag="M2A35_001526"
     CDS             15201..15947
                     /locus_tag="M2A35_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCB7197844.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            15972..16445
                     /locus_tag="M2A35_001527"
     CDS             15972..16445
                     /locus_tag="M2A35_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCB7197845.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            16442..16723
                     /locus_tag="M2A35_001528"
     CDS             16442..16723
                     /locus_tag="M2A35_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCB7197846.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            16800..18158
                     /locus_tag="M2A35_001529"
     CDS             16800..18158
                     /locus_tag="M2A35_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCB7197847.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPALSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRITRSTV
                     YA"
     gene            18151..19659
                     /locus_tag="M2A35_001530"
     CDS             18151..19659
                     /locus_tag="M2A35_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCB7197848.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPLEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            19649..19918
                     /locus_tag="M2A35_001531"
     CDS             19649..19918
                     /locus_tag="M2A35_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCB7197849.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            19950..20810
                     /locus_tag="M2A35_001532"
     CDS             19950..20810
                     /locus_tag="M2A35_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HCB7197850.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(20860..21219)
                     /locus_tag="M2A35_001533"
     CDS             complement(20860..21219)
                     /locus_tag="M2A35_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCB7197851.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(21216..21491)
                     /locus_tag="M2A35_001534"
     CDS             complement(21216..21491)
                     /locus_tag="M2A35_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCB7197852.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(21564..22688)
                     /locus_tag="M2A35_001535"
     CDS             complement(21564..22688)
                     /locus_tag="M2A35_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7197853.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPELGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(22688..25423)
                     /gene="rbbA"
                     /locus_tag="M2A35_001536"
     CDS             complement(22688..25423)
                     /gene="rbbA"
                     /locus_tag="M2A35_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCB7197854.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(25420..26487)
                     /locus_tag="M2A35_001537"
     CDS             complement(25420..26487)
                     /locus_tag="M2A35_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCB7197855.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(26853..28475)
                     /locus_tag="M2A35_001538"
     CDS             complement(26853..28475)
                     /locus_tag="M2A35_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCB7197856.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(28737..30343)
                     /locus_tag="M2A35_001539"
                     /pseudo
     CDS             complement(28737..30343)
                     /locus_tag="M2A35_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            30727..31779
                     /locus_tag="M2A35_001540"
     CDS             30727..31779
                     /locus_tag="M2A35_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCB7197857.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(32095..33297)
                     /locus_tag="M2A35_001541"
     CDS             complement(32095..33297)
                     /locus_tag="M2A35_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCB7197858.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            33529..35028
                     /gene="pitA"
                     /locus_tag="M2A35_001542"
     CDS             33529..35028
                     /gene="pitA"
                     /locus_tag="M2A35_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCB7197859.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(35099..35434)
                     /gene="uspB"
                     /locus_tag="M2A35_001543"
     CDS             complement(35099..35434)
                     /gene="uspB"
                     /locus_tag="M2A35_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCB7197860.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            35825..36259
                     /gene="uspA"
                     /locus_tag="M2A35_001544"
     CDS             35825..36259
                     /gene="uspA"
                     /locus_tag="M2A35_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCB7197861.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            36577..38046
                     /gene="dtpB"
                     /locus_tag="M2A35_001545"
     CDS             36577..38046
                     /gene="dtpB"
                     /locus_tag="M2A35_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCB7197862.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(38095..38847)
                     /gene="rsmJ"
                     /locus_tag="M2A35_001546"
     CDS             complement(38095..38847)
                     /gene="rsmJ"
                     /locus_tag="M2A35_001546"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCB7197863.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(38853..40895)
                     /gene="prlC"
                     /locus_tag="M2A35_001547"
     CDS             complement(38853..40895)
                     /gene="prlC"
                     /locus_tag="M2A35_001547"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCB7197864.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            41098..41940
                     /gene="rlmJ"
                     /locus_tag="M2A35_001548"
     CDS             41098..41940
                     /gene="rlmJ"
                     /locus_tag="M2A35_001548"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCB7197865.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            42012..43364
                     /gene="gorA"
                     /locus_tag="M2A35_001549"
     CDS             42012..43364
                     /gene="gorA"
                     /locus_tag="M2A35_001549"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCB7197866.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(43418..43501)
                     /locus_tag="M2A35_001550"
     CDS             complement(43418..43501)
                     /locus_tag="M2A35_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCB7197867.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            44010..44363
                     /gene="arsR"
                     /locus_tag="M2A35_001551"
     CDS             44010..44363
                     /gene="arsR"
                     /locus_tag="M2A35_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCB7197868.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            44417..45706
                     /gene="arsB"
                     /locus_tag="M2A35_001552"
     CDS             44417..45706
                     /gene="arsB"
                     /locus_tag="M2A35_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCB7197869.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            45719..46144
                     /gene="arsC"
                     /locus_tag="M2A35_001553"
     CDS             45719..46144
                     /gene="arsC"
                     /locus_tag="M2A35_001553"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCB7197870.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(46267..46563)
                     /locus_tag="M2A35_001554"
     CDS             complement(46267..46563)
                     /locus_tag="M2A35_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7197871.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            46671..47672
                     /locus_tag="M2A35_001555"
     CDS             46671..47672
                     /locus_tag="M2A35_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCB7197872.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            48252..49367
                     /locus_tag="M2A35_001556"
                     /pseudo
     CDS             48252..49367
                     /locus_tag="M2A35_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49721..50287
                     /gene="slp"
                     /locus_tag="M2A35_001557"
     CDS             49721..50287
                     /gene="slp"
                     /locus_tag="M2A35_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCB7197873.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            50443..50973
                     /locus_tag="M2A35_001558"
     CDS             50443..50973
                     /locus_tag="M2A35_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7197874.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(51015..51662)
                     /locus_tag="M2A35_001559"
     CDS             complement(51015..51662)
                     /locus_tag="M2A35_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCB7197875.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(51726..52052)
                     /gene="hdeB"
                     /locus_tag="M2A35_001560"
     CDS             complement(51726..52052)
                     /gene="hdeB"
                     /locus_tag="M2A35_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCB7197876.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(52168..52500)
                     /gene="hdeA"
                     /locus_tag="M2A35_001561"
     CDS             complement(52168..52500)
                     /gene="hdeA"
                     /locus_tag="M2A35_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCB7197877.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            52755..53327
                     /gene="hdeD"
                     /locus_tag="M2A35_001562"
     CDS             52755..53327
                     /gene="hdeD"
                     /locus_tag="M2A35_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCB7197878.1"
                     /translation="MLYIDKATILKFDLEMLKKHRKAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            54126..54653
                     /gene="gadE"
                     /locus_tag="M2A35_001563"
     CDS             54126..54653
                     /gene="gadE"
                     /locus_tag="M2A35_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCB7197879.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(54654..54932)
                     /locus_tag="M2A35_001564"
     CDS             complement(54654..54932)
                     /locus_tag="M2A35_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197880.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            54992..56149
                     /gene="mdtE"
                     /locus_tag="M2A35_001565"
     CDS             54992..56149
                     /gene="mdtE"
                     /locus_tag="M2A35_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCB7197881.1"
                     /translation="MNRRRKLLIPLFFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            56174..59287
                     /locus_tag="M2A35_001566"
     CDS             56174..59287
                     /locus_tag="M2A35_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCB7197882.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(59650..60378)
                     /gene="gadW"
                     /locus_tag="M2A35_001567"
     CDS             complement(59650..60378)
                     /gene="gadW"
                     /locus_tag="M2A35_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCB7197883.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(60746..61570)
                     /gene="gadX"
                     /locus_tag="M2A35_001568"
     CDS             complement(60746..61570)
                     /gene="gadX"
                     /locus_tag="M2A35_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCB7197884.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT        15368 a        15541 c        15064 g        15972 t
ORIGIN      
        1 gctgcgcttt ttcactttca gcaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
       61 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc gacggcttgt tcagaagcct
      121 tcacaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
      181 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtt
      241 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
      301 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
      361 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
      421 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
      481 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
      541 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
      601 ctggggctgg aagcgttatc gcgctacgct gcgggggcca cgttgattga gatggatcgc
      661 gcagtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
      721 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
      781 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
      841 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
      901 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
      961 ctgtatcaac gcgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
     1021 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctgaa
     1081 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc aactggcgct
     1141 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
     1201 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
     1261 accttattgt tcgcctacaa agctgacaaa gcgcgttcct ttatagctca gcgtaccttt
     1321 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
     1381 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
     1441 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
     1501 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
     1561 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttgct
     1621 catctttatc cctcatcgga aaatgcggaa ataagcatac cctgccagtt atggtgttgt
     1681 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattccg cttacgaaaa
     1741 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
     1801 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
     1861 taacccttct tctcctgctg ttactggcct ggcaagcgcc gatgttcgac gccattagct
     1921 atctggtgct ggcagggctg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
     1981 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
     2041 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
     2101 tgggcgcgct gttactggcg attatctgga cgcggctgga agagtatcgt tggcctatct
     2161 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
     2221 gtccgaccgc tccggcactc tctgcgtttg ttggcgcttc attgctgttt atcagtaact
     2281 ttgtctggct gggtagccac tatcgccgcc gcttccgggc agataacgcg attgctgcgg
     2341 cctgctactt tgccggtcat ttcctcatcg tccgctcgct gtatctctga taaaacttga
     2401 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
     2461 gccatgtcga ctcctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
     2521 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgttc cagctcgcca
     2581 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
     2641 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggtgt gaatcaggtg
     2701 caggtactgt tcgccaccga aaaactggtg gtcgatgccg acaatgacat ccgtgcccag
     2761 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
     2821 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgctaatcgt gatgatggca
     2881 atcagctggg gtctggagca atttaatcat ccgtttgggc aactggcgtt tatcgcgacc
     2941 acgctggttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
     3001 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
     3061 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
     3121 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
     3181 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
     3241 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
     3301 gccagttttg atgaaagcgc cctgaccgga gaatccattc cggtggagcg cgcgacgggc
     3361 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
     3421 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
     3481 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
     3541 gccgtcgccc tgctggtgac gctggtgcca ccgctgctgt ttgccgccag ctggcaggag
     3601 tggatttata aagggctgac gctgctgctg attggctgcc cgtgtgcgtt agttatctcc
     3661 acgcctgcgg cgattacctc cgggctggcg gcggcagcgc gtcgtggggc gttgattaaa
     3721 ggcggagcgg cgctggaaca gctgggtcgt gttactcagg tggcgtttga taaaaccggt
     3781 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
     3841 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcag
     3901 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
     3961 gcgctggtcg ggtctggcat tgaagcgcag gttaacggtg agcgcgtatt gatttgcgct
     4021 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
     4081 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
     4141 accctgcgcg ccgacgcagc aactgccatc agtgaactga acgcgctggg ggttaaagga
     4201 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
     4261 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
     4321 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
     4381 gccattggga ttgcaatggg cagtggcaca gacgtggcgc tggaaaccgc cgacgcagca
     4441 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
     4501 gccaatatcc gccagaacat cactattgcg ctggggctga aagggatctt cctcgtcacc
     4561 acgctgctag ggatgaccgg actatggctg gcggtgctgg cagataccgg tgcgaccgtg
     4621 ctggtaacag cgaatgcgtt aagattgttg cgcaggagat aaggcaaacc gatcgcaaca
     4681 ttgagcgcga tcggtcccct cgctcctctg gggagagggt tagggtgagg ggaaaaggcg
     4741 gcatcgaagc caatctttcc ccatcaaccg cctttacgaa tcaaataacg ataaggcagt
     4801 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
     4861 cgggtcgtcg ccggatcgtc ggcgataatc agtaaagttt cgccaggctg catattgcgc
     4921 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
     4981 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
     5041 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
     5101 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
     5161 tgttaagata acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
     5221 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
     5281 ttatttcatc tgctggtgat cacctccagt aactatctgg tccagcttcc ggtctccatt
     5341 tttggttttc ataccacctg gggcgcgttt agttttccgt ttatctttct cgcgaccgac
     5401 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
     5461 cctgcgttat taatctccta tgtcatctcg tcgctgttct atatgggttc atggcaggga
     5521 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
     5581 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcaaagtcgc
     5641 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagtga cacgctggcc
     5701 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
     5761 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
     5821 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
     5881 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
     5941 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
     6001 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
     6061 tcggtcgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
     6121 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
     6181 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
     6241 ccgaaagaag atctggcggt gctggcgaag cgtctggaag atcagcaacg tagccgcgat
     6301 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
     6361 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
     6421 gtgggtaatc aactgctgac catgcaaatt acgctgcccg ctgacgatca gcaaaaagcg
     6481 cagaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
     6541 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
     6601 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
     6661 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
     6721 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
     6781 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
     6841 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
     6901 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
     6961 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
     7021 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
     7081 cagccagacg ctcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
     7141 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
     7201 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
     7261 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
     7321 cacgccaacg ccccacaggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
     7381 cacgcgtccc aggcaaagta ataacaggct gatgacgggc agactggcgg ttaatcctgc
     7441 cgtcagatat cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatattttt
     7501 ggggcccagc aaatcggcgt aacgtccggc atgaggacgg ctcagcaagg tggcgaaata
     7561 ttgcaggctg ataaccaatc ctgcccagaa agcgctaaag cccatcacat catggacata
     7621 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
     7681 tatagagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
     7741 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccacgt gctgacgtga
     7801 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgctacactt aatacaaaaa
     7861 gtcacaagga agccccaatg gaaacccctc aacccgataa aacgggcatg cacattctgc
     7921 tcaagctggc ctcgctggta gtgatcctcg cgggcattca cgcagcggca gatatcattg
     7981 tgcagctgtt actggcgctg ttttttgcca tcgtcctcaa cccgctcgtc acctggttta
     8041 ttcgtcgggg agtacaacgc cccattgcca ttacgattgt ggtggtggtg atgctgatcg
     8101 cactaactgc gctggtcggc gtactggcgg catcgtttaa cgaatttatc tctatgctgc
     8161 cgaagtttaa taaggagttg acgcgcaaac tttttaaatt gcaggagatg ttgccttttc
     8221 ttaatttgca tatgtcgccg gagcgaatgc tgcagcggat ggactcggaa aaagtggtta
     8281 ccttcaccac agcgctaatg accgggcttt ccggggcaat ggcgagcgtg cttttgctgg
     8341 tgatgaccgt agtttttatg ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg
     8401 cgctgaataa cccacagatt cacatcgcgg gattacaccg cgcacttaaa ggcgtttcgc
     8461 actatcttgc attgaagacg ctactcagtt tatggacagg tgtcatcgtc tggctggggc
     8521 tggagctgat gggggtacag tttgcgctga tgtgggcagt actggcgttt ttgcttaact
     8581 acgtgcccaa tatcggcgcg gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt
     8641 ttaatggtat ttacgaatgt attctggtcg gcgcattgtt tttagttgtc catatggtca
     8701 tcggcaatat tttagagcca cggatgatgg gccatcgcct ggggatgtcc accatggtgg
     8761 tatttctttc attgttaatc tggggatggc tgctcggccc ggtagggatg ctactttcgg
     8821 tgccattaac cagcgtgtgt aaaatctgga tggaaacgac gaaaggtggc agcaaactgg
     8881 cgattttact ggggccgggc agaccgaaaa gtcggttacc gggatgaggc gacaagtgat
     8941 acgatacgca ctttcatttt ccattaaacg ttggccctga tatgtatcgg atagttctgg
     9001 ggaaagtttc gaccttaagc gcagctccac tgccaccggg tttacgcgag caagcaccgc
     9061 aaggtccacg acgcgaacgc tggctggcgg ggcgtgcatt gctttcgcac acgctttccc
     9121 cgctaccgga gatcatctat ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc
     9181 tatggttcaa cttaagccat agcggtgacg atatcgctct gctgttgagt gatgaaggcg
     9241 aagtcggctg cgatatcgaa gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg
     9301 ccgtattcag cctcggggaa cacgctgaga tggacgccgt gcatcctgat cagcaactgg
     9361 aaatgttctg gcgcatctgg acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg
     9421 cctggcaaat cgtcagcgta gacagcacct atcactcctc gctgtcagtc agccattgtc
     9481 agcttgaaaa tttaagcctg gcgatctgca cccccacccc ctttacgctc accgccgaca
     9541 gtgtgcaatg gatcgattca gttaactgat ccgcccaccc gactgcccat ctattgatcc
     9601 agaacaggta atcagtatga cgaatactta aaatcgtcat acttatttcc gccatctatt
     9661 ttaatccatt ggggttacca tgctctccac actccgccgc actctatttg cgctgctggc
     9721 ttgtgcgtct tttatcgtcc atgccgctgc accagatgaa atcactaccg cctggccggt
     9781 gaatgtcggg ccactaaacc cgcaccttta cacgcctaac cagatgttcg cccagagcat
     9841 ggtttatgaa ccattggtga aatatcaggc agacggttcg gtgatcccgt ggctggcaaa
     9901 aagctggact cattcagaag atggtaaaac ctggaccttc accctgcgtg atgacgtgaa
     9961 attctccaac ggtgaaccgt tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct
    10021 cgataaccgt caacgtcacg cctggctgga gctggcaaac cagattgttg atgttaaagc
    10081 actcagtaaa acagagctgc aaattaccct gaaaagcgcc tactatcctt tcctgcaaga
    10141 actggccctg ccccgtcctt tccgttttat cgctccctcg cagtttaaaa accatgaaac
    10201 catgaacgga attaaagcgc cgattggcac cggaccatgg attttgcagg aatcgaaact
    10261 gaatcagtac gatgtcttcg tccgtaacga aaactactgg ggcgaaaagc cagcgattaa
    10321 aaagatcacc tttaacgtca tcccggaccc gactacccgc gcggtggcgt ttgaaactgg
    10381 cgatatcgac ctgctgtacg gaaacgaagg gttattaccg ctcgatacct tcgcccgctt
    10441 tagccagaat ccggcttacc acacccaact gtcacagccg atcgaaaccg tgatgctggc
    10501 gctcaatacc gccaaagccc ccaccaatga gctggcagta cgtgaagctc ttaattacgc
    10561 ggtaaacaaa aaatcgctga ttgataacgc gttgtatggc acccagcagg tcgccgacac
    10621 cctgtttgcc ccttctgtgc cctacgccaa cctcggcctg aaaccgcgcc agtacgatcc
    10681 gcagaaagcg aaagcgttgc tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat
    10741 ccgcgagaaa aatggtcagc cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt
    10801 aagcaaatcg atggcggaaa tcattcaggc tgatatgcgc cagattggcg cagatgtctc
    10861 gctgattggc gaagaagaga gcagtatcta tgctcgtcag cgcgacggtc gttttggcat
    10921 gattttccac cgcacctggg gcgcgccata cgatccacac gccttcctca gttcaatgcg
    10981 cgtaccgtca cacgctgact tccaggcaca gcaaggatta gccgacaaac cgctgattga
    11041 taaagagatc ggcgaagtgc tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg
    11101 cgacattctg acccgtctgc atgacgaggc ggtttatctg cctatcagtt acatctcaat
    11161 gatggtggta tcaaaaccgg agctgggtaa catcccctac gcgccgatcg ccactgaaat
    11221 tccgttcgaa cagattaaac cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt
    11281 ctgctgctga tcccgatggt gctcgccgcc tcggtgatca tttttctgat gctgcgcctc
    11341 ggtaccggcg acccggcgct cgattatctg cgtttatcta acctgccgcc gacgccggag
    11401 atgctggcct ctacccgcac catgctcggg ctggatcagc cgctgtacgt ccagtacggc
    11461 acctggttgt ggaaggcgct gcatcttgac tttggtatct cattcgccag ccaacgcccg
    11521 gtgctggacg atatgctgaa cttcctgccc gccacgctgg aacttgcagg tgcggcgctg
    11581 gtattaattc tgctcacttc cgtaccgctc ggtatctggg cggcgcgcca tcgcgaccgt
    11641 ctgccggatt tcgccgtacg tttcatcgcg tttcttggcg tgtcgatgcc taacttctgg
    11701 ctggcgtttt tactggtgat ggcgttttcg gtgtatctgc aatggctacc cgcgatgggt
    11761 tacggcggct ggcagcacat cattttgcct gcggtttcca ttgcctttat gtcgctggcg
    11821 attaacgcgc gtttactgcg cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc
    11881 tgggcgcgtc tgcgcggcct gaacgacaaa cagaccgaac gtcgccacat cctgcgcaat
    11941 gcctcgctgc cgatgatcac cgccgtgggg atgcacatcg gcgaactgat tggcgggacg
    12001 atgattatcg aaaacatctt tgcctggccg ggcgtcgggc gctatgcggt gtcggcgatt
    12061 tttaaccgtg actatccggt gatccagtgc tttacgctga tgatggtggt ggtttttgtg
    12121 gtctgtaatt tgattgtcga tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat
    12181 gaaggagcgc acgcgtgaac tttttcctct cttcccgctg gtcggtacgc ctggcgctga
    12241 tcattatcgc cctgctggcg ctgattgcgc tcaccagcca gtggtggctg ccgtatgacc
    12301 cccaggcgat tgatttgccg tcgcgcctgc tttcgccgga tgcgcagcac tggctgggca
    12361 ccgatcactt aggtcgcgat attttctcgc ggctgatggc agcgacccgc gtgtcgctcg
    12421 gttcggtaat ggcctgcctg ctgctggtgc tgacattagg gctggttatt ggcggcagcg
    12481 ccgggttgat tggcgggcgc gttgatcagg ccaccatgcg cgtcgccgat atgtttatga
    12541 ccttcccgac ctcgattctg tcgttcttta tggttggcgt gctcggcacc gggctgacca
    12601 acgtaattat cgccatcgcc ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagcc
    12661 tggtgatttc actacgccaa cgcgagtttg tgctggcgtc acggctttct ggcgcgggcc
    12721 atgtgcgggt gtttgtcgat catctggcag acgcggtgat cccttcgctg ctggtgctgg
    12781 caacgctgga tatcggccat atgatgctgc acgtcgcggg gatgtctttc cttggcctcg
    12841 gtgtgaccgc gccgaccgcc gaatggggcg tgatgattaa cgacgcgcgc cagtatatct
    12901 ggacccagcc gctgcaaatg ttctggccgg ggctggcgct gtttatcagc gtgatggcct
    12961 ttaacctggt gggtgacgca ctgcgcgatc atctggaccc tcatctggtg acggagcacg
    13021 cacactaatg ccgcaacaga ttgaactacg taatatcgcg ctacaggccg cgcagccgct
    13081 ggtacacggt gtatcgttaa ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag
    13141 cggcagcggg aaatcattaa cctgcgccgc gacgctgggc attttgcccg ctggcgttcg
    13201 ccagacggcg ggggaaattt tagccgatgg caaaccggtt tcgccttgcg ccctgcgcgg
    13261 catcaaaatt gccaccatca tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat
    13321 gcacacccac gcgcgggaaa cctgcctggc gttagggaaa cccgccgatg acgctacgct
    13381 taccgctgcc atagaagcgg tggggctgga aaacgccgcg cgcgtgctga agctgtaccc
    13441 gttcgagatg agcggcggca tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga
    13501 atcaccgttt atcatcgccg atgaaccgac caccgacctc gacgtggtag cacaggcgcg
    13561 catcctcgat ctgctggaaa gcattatgca aaaacaagcg ccgggaatgc tgctggtcac
    13621 ccataatatg ggcgttgtgg cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa
    13681 aattgtcgaa cagggcgatg tagaaacgct gtttaacgcc cccaaacata cggtgacgcg
    13741 cagcctggtt tccgctcatc tcgccctcta cggtatggag ctggcatcat gaccttactt
    13801 aacgtctgcg gcctttccca tcactatgcc cacggtggat ttagcggaaa acatcaacat
    13861 caggcggtgc tgaataacgt ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg
    13921 ggacgcagcg gctgcggaaa aagcaccctc gcgcggctgc tggtaggctt agagtctccc
    13981 tcacaaggga atattagctg gcgcggcgaa ccgctggcga aactcaatcg cgcccagcgt
    14041 aaagcgttcc gccgcgatat tcagatggta tttcaggact ccatcagcgc cgtgaatccg
    14101 cgcaaaaccg tgcgcgagat cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa
    14161 tccgaacaac tggcgcgcgc cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt
    14221 ctcgacaaac gcccaccgca gttaagcggc ggccagctcc agcgcgtctg cctggctcgc
    14281 gcgctggcgg tcgaaccgaa actactgatt ctggatgaag ccgtttctaa ccttgatctc
    14341 gtgttacagg cgggcgtcat tcgcctgctg aaaaagctac aacaacagtt tggcaccgcc
    14401 tgcctgttca tcacccacga cttacgcctg gtggaacgct tctgtcagcg ggtgatggtg
    14461 atggacaacg gacaaatcgt cgaaacccag gtggtgggag ataaattaac cttttcctct
    14521 gacgccggac gtgtgctaca aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc
    14581 acagaaaagg tttaacgcaa atgcaacgag tcaccatcac gcttgatgac gatttactgg
    14641 agacgctgga cagcctgagc cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg
    14701 atattctgcg tagcgccctg gcgcaagaag ctacccagca gcacggcaca caaggtttcg
    14761 cggtgctgtc gtatgtgtat gaacacgaaa aacgcgactt agccagccgt attgtttcca
    14821 cccagcatca tcaccacgac ctctccgtcg ccacgctgca tgtgcacatc aaccacgacg
    14881 actgtctgga aatcgccgtg ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg
    14941 acgttatcgc ccagcgcggc gtgcggcacg ggcatttgca gtgcttgccg aaggaagatt
    15001 aacaccgacc aacgcgccgt tatttcctta cggcgcagta aacttctgac aaatatcgac
    15061 tcacgtcaca tttttttgat cacgatttgg tccctcaaaa tgtgagacgc ttcgatcctc
    15121 tttttctccc gttcacctac tatacgcttg aagttgtcca tgattgtcca tagacaactg
    15181 taaatgttca ggtgcgcgcc atgaaatcat taagtaagtc gtcacaaata ccgctctatc
    15241 aacaagtggt ggagtggata agagaaagta tttataccgg agatctggtg gaagacgatc
    15301 gcattccttc ggaataccag attatggata tgctggaagt gagtcgggga accgttaaaa
    15361 aagcggtcgc ccaactggta aaagaaggcg tgttaataca ggtccagggt aagggaacat
    15421 ttgtcaaaaa agagaacgtg gcatatccgt taggtgaagg attattgtca ttcgcggaat
    15481 cgctggaaag ccagaaaata cattttacca ctgaagttat tacgtcacgg attgaatcgg
    15541 ctaatcgtta tgtggcagag aaattaagaa taacgcccgg tcaggatatt ctttaccttg
    15601 aacgcttacg ctcaattggt gatgaaaaag cgatgctgat agagaaccgt atcaatattg
    15661 agctatgccc cggtattgcg gaaatcgatt ttaatcaaca caatttattt ccaacaatag
    15721 aaagtttgtc gcaaagaaaa attcgttact cggaaagtcg ctatgccgcg cgattaattg
    15781 gtaatgaacg tggtcatttt ttagatatca gtgaagacgc acccgttttg catctggagc
    15841 agttagtctt tttctcccga ggattaccca ttgagtttgg caacgtctgg ttaaaaggaa
    15901 ataaatatta tcttggcacc gtactgcaac ggcgggaagt gagttaagta cactttttta
    15961 agaggagtaa aatgcaaggc atacattttc aggaaaatta tattcaacga cttccggcag
    16021 ggttaagcgt tgaacagatc atccgtcagt tagcgcaacc gttggttgcc gccgagctgg
    16081 tcgtccctga ttttgccgat cacgtactga aacgtgaggc gacgtaccca acggggctgc
    16141 ctaccgaacc accgtgcgtc gctattccgc acacggacca taaacatgtc aggcacaatg
    16201 caattgccgt cggcatcctg ccggaaccgg tagagtttgc cgatatgggc ggcgatcctg
    16261 accccgtacc tgtcagggtg atttttttgc tcgccttaag cgaaagtaat aaacaattaa
    16321 atgccctggg ctggattatg gaaatgattc aggatacgcc ctttatgcaa gccctgcttg
    16381 cgatggaaac aacagaaata cacacagtaa tcttaaacaa aatgaaagaa cgaggtgaaa
    16441 tatgagtcaa accattttat ttgtctgcgc cacgggtatt gccacatcca cggcggtaac
    16501 agaaaaagtc atggaatatt gtaaagagca cggtcttaac gtcaattatt cccaaaccaa
    16561 cgttgcctct ttacctggaa atactgatgg cgtagcactg gtagtctcaa cgactaaagt
    16621 gccttatgaa ctcgacgtcc cggtagttaa cggcttgccg attattaccg gtattggcga
    16681 agagaaagta ctggcgcaaa ttgtttcgat ccttaaaaat taattaataa taattaattt
    16741 caacccactc catcaggcgc taacgcgagg tgcgctctcg cctgaaaaag aggtttatta
    16801 tgaacgatat cgcgcatacc ctctataata ttgtgcaata tatattggga tttggcccga
    16861 cggtaatgtt gccgttggtg ttatttattc ttgctctctg ttttaaagta aaacccgcta
    16921 aagccttacg ttcgtcatta acagtcggca ttggttttgt cggtatttat gccattttcg
    16981 atattctgac cagcaatgtc gggccagcgg cccaggcgat ggttgaacgc accggaatta
    17041 atttaccggt ggtagattta ggctggccgc cgctttccgc tattacatgg ggttcgccaa
    17101 ttgccccgtt tgttatcccc ctgaccattc tgattaacgt ggcgatgctg gcgttaaata
    17161 aaacccgtac cgttgacgta gatatgtgga actactggca ttttgccctt gctggtacgt
    17221 tggtttatta cagcaccggc agcctgttct ttggtttgct ggcggcggcg attgctgcgg
    17281 tggtagtact taaactcgcc gactggtctg cgccactggt acaaaaatac tttggactgg
    17341 aagggatctc attgccggcg ctctcttcgg tggtgttctt cccggtcggt ctgctggtcg
    17401 acaaaatcat cgaccatatc cccggcctca atcgtattca tatcgacccg gaaaccgtac
    17461 agaaaaagtt tggcatcttc ggcgaaccga tgatggttgg cactattctg ggcatactgc
    17521 tcggcgtgat tgcaggatac gatttcaaaa aagtgttgct gcttggcatc agcattggcg
    17581 gtgtgatgtt catcctgcca cgcatggtac gcatcctgat ggaaggttta ttaccgctgt
    17641 ctgaagccat taaaaagtat ctcaatgcca aataccctga ccgtgacgat ctctatatcg
    17701 gcctggatat cgccgttgcc gtaggtaacc cggcgattat ctccaccgcc ctgctgctga
    17761 cgcctatctc ggtctttatc gcgtttgtcc ttccgggtaa tgaagtcctg ccgcttggcg
    17821 accttgccaa cctggcggta atggcgtcga tgattgcttt agccagccgt ggcaatattt
    17881 tccgcaccgt tctggcggcg atcccggtga ttattgccga cctgtggatt gcgactaaaa
    17941 tcgcaccgtt tattaccggc atggcgaaag acgttaactt caaatttgcc gaaggctcca
    18001 gcggccaggt ttccagtttc cttgatggcg gtaacccgtt ccgcttctgg ctgctggaaa
    18061 tcttcaacgg caatatcatc gccattggtc tggtgccggt tatcgccctg gtactgtatg
    18121 gcattttccg aataacgcgg agcacggttt atgcctgata acagcgcagc tatcgttatc
    18181 gatattggca ccaccaattg caaagtcacc tgcttttcct gcctggacgc aacgacgttg
    18241 ggcgcgcata aattcgtgac ggcaaagcag atctccccac agggcgatgt cgatttcgat
    18301 atcgacgccc tctggcagga ggtccgccag gcgatagcgc aactgaacgc cgcttcgccg
    18361 ctgccagtca gacggatcag cattgccagt tttggcgaat caggcgtgtt ccttgacgag
    18421 catggcgaga tcctgacgcc aatgctggca tggtatgacc gtcgcggtga agagtatctg
    18481 gcaacgctta gcgaggcaga cagtgcagca ctttatgaca tctgcggtct accactacac
    18541 agcaattact ctgccttcaa aatgcgctgg ttgctggaac attacccgct gcgtaatcgc
    18601 cgcggcctgc gctggctaca tgcgccggaa gtgctgctct ggcggctgac tggcgaacag
    18661 cgcacggata tcaccttagc cagccgcacg ctgtgtctgg acgtgcgcaa aggcgaatgg
    18721 tcagcgaaag cggcggcgtt gttacacgtt ccctgtacgg catttgcgcc attggtgcag
    18781 ccaggcgagc acgccggatg ggtcagcgag tcactttgca agacgcttgg gttctcgcaa
    18841 ccggtcagcg tgacgctggc cggacatgac catatggtgg gtgcgcgagc gttgcagatg
    18901 atgccaggcg atatccttaa ctcgacgggg accacggaag gcattctgca actggataca
    18961 caaccgacgc tggatgaaca ggccaaacgt gacaagctgg caaacggctg ttactcactt
    19021 gccaaccagt tcaccctgtt tgcgtcgctg cccgtgggcg gtttcgcgct ggagtggctg
    19081 cgcaacacgt tccggctaac cgatgaggag atcgccgcat cacttactcg cggacatgct
    19141 gattatctgg cggggaattg gttgctcgat gacattcccg tctttattcc acatcttcgc
    19201 ggttcgggtt cgccctataa aaatcgccat acccgtggat tattttatgg gcttggcgat
    19261 acgttaagta tcgacatgtt aattgccagc gtatcactgg gattaaccat ggaatttgcc
    19321 aactgcttcg cctgttttaa cgtgcctggc accagcgcgt taaaagtgat cggtccggca
    19381 acccataatc ctctttggct gcaattaaag gcggatattt tacagcgtcc gcttgaagca
    19441 attgcattta acgaggcggt ttctgtcgga gcattattaa ccgccgcacc ggatattcca
    19501 ccgccgccag tcgctatagc ccaacgtttg ttaccgaatc gggcgagata ccatcaatta
    19561 cagcgttatc agcacaaatg gaaaagctgg tatcagttga aattacaaca agaaggcgtg
    19621 atgccattac atcatcggga ggaacactat gttgaataaa accgtggagg tcagaaacag
    19681 tactggcctg catgcacgtc cggcggcctg tctggcgaaa gccgccaaaa aatatagctg
    19741 taaagtgacg ctgcactatg agggtaatga tattaatgcc accagcatga tgaatattat
    19801 gcgcgccgga ataaaaggcg gaaaaacggt ggaaatacgc tgcgacggtg atgatgaaaa
    19861 cgaggccata cagactctca ccgacttatt tcgcgaccgc ttcggtgaag ctgaataatt
    19921 aactgatatt aaaagatgag gttttcaata tgccattagt taatggacgg atactgctcg
    19981 accgcattca ggaaaaacgg gtattagcgg gcgcatttaa taccaccaat ctggagacga
    20041 caatttcaat tttaaacgct attgagcgtt cgggattacc caacttcatt cagattgcgc
    20101 caaccaatgc acaactctcg ggatacgatt acatttatga aatcgtgaaa cgtcacgccg
    20161 ataaaatgga tgttccggtc agtcttcatc tggatcacgg gaaaactctg gaggatgtga
    20221 aacaggcggt gcgcgcgggc tttacctcgg tcatgattga cggtgccgca ttctcttttg
    20281 aagagaatat cgctttcacc caggaagctg tcgatttctg taaatcctat ggtgtaccgg
    20341 ttgaagcgga gctgggggcg attctcggca aagaagatga tcatgtcagc gaagccgatt
    20401 gcaaaacgga accggaaaag gtgaagacct ttgtcgagcg taccggttgc gacatgctgg
    20461 cagtttctat tggcaacgtt catggcctgg acgatatacc gcgtatcgat atccccctgc
    20521 tgaagcgcat tgccgaagtc tgtccggtgc cgttggttat tcacggcggc tccggtatcg
    20581 ccccggagat attacgcagc tttgttaatt atcgcgtcgc taaagtaaat atcgccagcg
    20641 atctgcgcaa agcgtttatc accgccgtcg gcaaagcgta tgtgaataat cacaacgagg
    20701 cgaatctggc acgagtcatg gcatcagcaa aaaacgccgt cgaggaagat gtgtatagca
    20761 aaatcctgat gatgaatgag gggcatcggt tggttagaaa ggcatcttaa gagccacaaa
    20821 aatatagcgg cgcaacagaa attgttgggc cgctataagt tataaatgat tcaaacgctc
    20881 attcagtgtt tcaataatat aggtattaac agagacttgt tgctgcgcag ccgcattatt
    20941 aaggcgttca ctcagcgatt ctggatagcg taacgtaaag gtcttaatct tttctgtacg
    21001 cgcatacggc tcaatacccg cagctttaca atcctcaaga tactctagca gggaaagttc
    21061 cccttctttt tgcagcccct gaatgctgtc agagacaaaa tcacaataac ctgacaagcc
    21121 caggaatttg ccacgaaatg cattcagttc tggcacgtaa gtgataactg caggttgccc
    21181 ggcaatctcc atagagtttg gcgtcttcag tttgatcatg gttttacccc tatacttagt
    21241 aaccagtcac gcacgctttc tacagcgcct ttatcggtat ctggcgacgg atgaggccga
    21301 tggaaacacg caacgctcat atttagtatg aacttacaac gcgaacctct tccttcctta
    21361 atttctccgc ctaatgcttt aaccagtgac tctatatcag accacttgat cccttgagga
    21421 acaggagttt taaatatttg atccaatgta tttttttgtt tcttccgtaa agcctgtacc
    21481 ttggttctca tggcatgtcc ttatgacttc actttatgac atcaatagta tgacatcaca
    21541 gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct gaatcgcagc
    21601 agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg cggccagacg
    21661 atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa gtgtgtcgtc
    21721 ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg cgtggatcca
    21781 ccagaaagca tttgcaacgg tagcagcacc agaatcacca gcagccccag ttccggcatt
    21841 gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag actgagcgcc
    21901 acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac acctttcacc
    21961 atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga ccagatcttc
    22021 gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac cgtgccgtgt
    22081 tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt aatgttgttg
    22141 atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa gcgcatccgg
    22201 gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa gctgttcact
    22261 tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct catgcgcgtg
    22321 gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg ctgaaaatta
    22381 ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc catctcatcg
    22441 gcggtgatca tctccggtgg caaaaaccac gggcgataga agctgttaac gatccggttc
    22501 gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa cgatccaggc
    22561 gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag cgtcagcatc
    22621 gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat attgcgtaaa
    22681 tggcgcatca tccctcctgt tttttcagca gcaggatact taagcccatc accagcggga
    22741 tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc gccttcgaga
    22801 acgtcccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg atccaacgtc
    22861 caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc gccgggatca
    22921 atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag gtggagatca
    22981 gcagccccat tccggtggca atgatgatat acagcagcgc cgccagggtg agcgtcagga
    23041 aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc aggaaaaagt
    23101 tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg ctacgggtgg
    23161 tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc acgctaaggg
    23221 cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga acaatcgctg
    23281 gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc agcccgcttt
    23341 ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg tgcattgcct
    23401 gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc cagacgccga
    23461 gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc tcaatcgcca
    23521 ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg agcggcggct
    23581 gttcgataaa gtaacgggaa ccggagagat tgagcgtcca cgcctggcta ctgacggtct
    23641 ggtcgcggtc gagcacagca aagcgcaggt tttccacatc catactgatg ccgtaaccca
    23701 ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact ggatcgcgtc
    23761 gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta aatccctgac
    23821 gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg ctctgccctg
    23881 ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg cgtttctcaa
    23941 ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt gagatgcggt
    24001 cgcaacgttc cgcttcgttc atgaagtggg tggagatgaa gatagtcact ttgtcctggc
    24061 gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg acaccagaag
    24121 taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc agcgaaagcc
    24181 gctggcgaat gccgagcggc aatgactccg gcagaatatc ttcaacgtcg ttgagcttaa
    24241 aacgctcgct catttcagcc actcttgcgg gaatttccgc ttccgggatg tgaaacaaac
    24301 gggcatgtaa ctcaaggttt tgccgcacgg tgagttcgtt atagagcgaa aacgcctgcg
    24361 acatatagcc cacccgacgg cgggtatcga tatcttttgg atcaaccggt tgcccgaaca
    24421 gccacgcctc accttcgctg gcgggcagca gtccggtgag cattttcatg gtggtggatt
    24481 taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg cggaaattaa
    24541 cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct tcgatggcaa
    24601 tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc tggcgttgcg
    24661 cttgcggtaa cagatttata aatgcttctt ccagcgtagc gctttgcgtt tgctgccgta
    24721 gctcttcggc gctgccagtt gccagcactt ctccggcatt catcgctacc agccagtcga
    24781 agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg ctctgccgct
    24841 gacgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca acccccgttg
    24901 ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac agcccaagtt
    24961 tttgcttcat cccgccggag agtttccctg ccggacgatc gcgaaacggt gctaaccccg
    25021 tgctggtcag cagctcatta attcgcactt cccgctccgc tttgtcgtga ccgaacaggc
    25081 gagcgaaaaa atcgacgttt tcatacaccg acaaggtgtg gtagaggttt ttgcccagcc
    25141 cctgcggcat ccaggcgatg cgcgggcaga cgtcgcggcg atgcctcggg tcgcgcatat
    25201 cgccgcccag caccatcaca ttgccctgct caatgacgcg ggcaccggaa atcaacgaca
    25261 ataagctcga cttcccgacg ccatccggac caatcagccc gaccatacag cgggccggaa
    25321 tatcgagagt aatattgttc agcgcaacgg tttttccata atgctggctc acgcccgcca
    25381 gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca acctcaccac
    25441 gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc ccggtaaacc
    25501 ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg ctttgacgcg
    25561 gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga actgggcgac
    25621 actggcgaca aaactgatgg tcgccggaat acgcagatct ggcgcggcat cgaggatcag
    25681 ccgggcttca ccacccagtt tcagcgtgcc cgcctgttcg gttggcagga agaaggtcat
    25741 atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca gcacttcccc
    25801 tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt cgctgtcatc
    25861 gatatctgcg gcaatgcgcc gttcagtggc ttgtgccgct tcgacgcggg tttgcgcctg
    25921 aatgatattg gtacgtgccg cctctatagc ggctttagaa gcagaaacct gagcttttgc
    25981 cgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca gctgttgcgc
    26041 agaaatagcc cctcgttggg ccagtgaacg ggaacgcata tgacgttttg ctacggagtc
    26101 cagttctgcc tggcgttgat taaccagcga ctgcgcggcg cgagtttcgc tttgtcgttg
    26161 ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt gcgcgatggc
    26221 ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt caccttcgcg
    26281 aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt tgctggcgat
    26341 atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg gcacacccgc
    26401 cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga caacccacca
    26461 cgccagatgg cgcttactct tatccataat cggcccgcca taatccctgt aagcaaacga
    26521 cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg gataagaagg
    26581 aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg cttagtgaaa
    26641 acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac gcatactcat
    26701 aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat cgcgtttcac
    26761 gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag tctcaaccag
    26821 atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt gcgccttcta
    26881 atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca gggagtggtt
    26941 atctcaggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat tcttccagca
    27001 tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat acattctctt
    27061 ccaccattac cgttatgtgc aacaacttga tttataccta acatttgtga gcatgctaat
    27121 agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt aaaggatcga
    27181 caagagatag cccggctatt tttaaacttt ttgccattaa ttacagcatg gtgcttttta
    27241 ttgataattg ccgctaattc gaccggatcg agagcattgg tcatttcatc actagagact
    27301 gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc atgatgattt
    27361 gccattattt tgtatttggg gccatagaaa catacaggat aatcttttat cccagcgtag
    27421 gtatcctcat caagtggttt catgcgcgtg aagttctgca tatagttacc attgcaattc
    27481 gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat gcgttttaac
    27541 aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt ttggtcgcca
    27601 agaaacgtac atggcgtcac agcgtcttta ttaataacta aataaggctg aagactttct
    27661 aaaactatat ctcttctgtt cagaatactg attattgtat taacattagt gtcaagttgc
    27721 tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt aagcaactgg
    27781 ctaaggaaat tttctccttc agccatgatt ctcaaatgtc ccgttgcaaa aagcgcgtac
    27841 aaaactgcac cgactgagcc atcggtatcg ccaaaatagg ttggtctggt acttgtggga
    27901 tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actgggggtt tatcattgtt
    27961 tcgaatttaa tatcagatac attgaattgc gatattttcc agtcccagac gggtttgtac
    28021 gactcagtaa ctgcaacatc attagcatca tcatttactg tcatattatt aagtaaataa
    28081 acaatgctgc accaattaat atcggtatta tcaagatagc agccgtaatt tttactatta
    28141 attgtcacaa taaactgata attattatcc ctacgtagaa tatgagcgcg catgtccgat
    28201 agcatactat atttttctga atgattcgtt tgtatggtaa atgatatttt gtcatctcga
    28261 atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac ttcaccgagc
    28321 attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata cccaaacagt
    28381 tctttagtga agttagtaac atgctcttgc accatatcat ttgttgcgat tgtcgttgta
    28441 ctgtcgttag tgcctgccac agttccaatt ttcatctttt acctcatata attcaccgcg
    28501 aggaatgcat agttaacatt taatcttatg acatacacct catgttcgat ggaaaaataa
    28561 ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt atctcatagc
    28621 agagaaagtc cctaaagatt gtagaggcgt catcagaaca tgcctctaca atcgattaag
    28681 aaaacctatg aaatactggc aggattaaaa agacatacca ccgtattcat ggagttttaa
    28741 tgagattgaa aacaactaag ccctgaggaa taaattattt tattattttt atcacgtgct
    28801 tccttcaggg aatttagccc cataatacca ttatcaaatt gttgattctg atctaccccg
    28861 accccatgat gcccatattt ttgtctaatg tatttgctgg ggtcttcacc tttgagtgtc
    28921 tccccatata tgaagatacg tttacgtgct ggtctgaaat cagtagtgtt atcaaaataa
    28981 ttagcatata caggtgtggg tttcaaagta ttgcttcgga ttagatggaa tagaggatca
    29041 tccatgtgtt ctttatgttt tttattaaaa atattggcta attcctcagg atcagtagga
    29101 tttttcatct gattaaatgg aacctgcaaa gcaccgagac aataacattt ttttcttcat
    29161 ctcttattat tccgtgatga ctggttaaaa ctttcctttc tgagtcataa tgacaaacat
    29221 cgagcgtttt aattaagtta tacgtatcac cggcagagag tttataattt tctagtcatg
    29281 cagtatagtt accattaaaa ttgatttcat gatttctagc cagaacgatg acatttttat
    29341 taatacggct atctttatta ccctccatat ttcgcataga atttagcaaa gtaaggatat
    29401 atttatcacc aaaaatagta ctaaatctat cgcctgtatg atccccaagg aagatacaag
    29461 gggttatcgc atcgtccttt aaaaaaacat gttgatgcaa ctcattcaat atcatgtctc
    29521 tcgtatttaa taaattgtag agcaatttat tatttttatg tatgtatgca gatgaagcca
    29581 tgttttcgtg ttttaataat tcacataata aactccaacc cttttccctg atgcctatat
    29641 gccctgaaac gagcaatgca taaagtacag cgcctactct tccatctgta tcgccaaaat
    29701 aagccgtgtt ggcattttgt gggttaatca caccacagtg tttataaaag tctattttag
    29761 aaaacacaga tttaacatag ctatcaaact tatccttagg aaacgtctct ttagagacat
    29821 cccaacgcca gtcagcttta aaattaccgc taagaagagt aaagaaattt tcaggtatga
    29881 tagaaaggcc attaacttta taaattatgt ctgatttatc tccagagaaa ttaataaaat
    29941 aataaccatc cttacactca atcgtaacgg taatgggaga cgataatttt atcacatagt
    30001 tattgtctgg tataattgta aagataaacg tgttagtagt attcttggcg aaactattat
    30061 attcttgaat atctatagat ggctcactat tgtaaaacca tattttctcc atctgaagac
    30121 tatgttcatt taccctccag tgaccatgtt gcaggaaata gatgatattc aggcctaaat
    30181 cgttatctat ttgcgaaaag tcattcagca tattaactta aactgtgttt gatcgatatt
    30241 ttttatcaac cttgccatgg tgttgatgac tgttaacttt atctgaatgc ttatcttgat
    30301 ggtttgaaga gaagtgctta aatatattgt gaaatacttt cataaataac ctgtaatatt
    30361 tttacttgtt agtaacaaaa taaacaatac atgtctgaaa gacatattta cgatgataca
    30421 aagcaataat acttatatgt tcgatgattt taagcaccga gactatccgg tcttttacta
    30481 aataatggaa aacaatccat ccctggagga taattgtaaa aaaaacaaca gaataagaat
    30541 taatcttagg ataaatattt atttatcatg gccttttaac cggtaaaaaa tggcactcgg
    30601 cacagtaaac cccgctatag ttatgctatg catttgtctt taatgatatc tcgtcgttat
    30661 attaacaggc tgaaattatc attaatgcac atttcattat tagcagggat acctgaggag
    30721 tttaaagtga acatatatat cgggtggctt ttcaaattaa tccctttgat tatgggatta
    30781 atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaaagcga gtatttcgtc
    30841 gcgggtcatg tgctgatttc tctggcggcc atatgcctgg cattattcac taccgcattt
    30901 attatcattt cgcagctcac gcgcggcgtt aatacgtttt acaatacatt gttccccatt
    30961 attggctatg cggggtcaat tatcaccatg atatggggtt gggcactgtt agcaggcaat
    31021 gatgtgatgg cagacgagtt tgtcgccggc catgttattt tcggcgttgg tatgattgcc
    31081 gcctgtgtat cgacggtggc agcgtcatcc ggtcactttc tgctcattcc caaaaatgca
    31141 gcggggagca agagcgatgg aacgccggta caggcttatt cttcattaat cggtaattgc
    31201 ctcattgccg ttcccgtttt actcaccctg ctcggtttca tttggtctat tacgctgtta
    31261 cgtagtgctg acataactcc gcattatgtc gcgggtcacg tattgcttgg gttaaccgca
    31321 atctgtgcct gtctaattgg ccttgttgcc acaattgtcc atcaaacacg taatacgttt
    31381 tcaactaaag aacactggct gtggtgttat tgggttattt ttctcggctc aatcacggta
    31441 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
    31501 attcttattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
    31561 ctggtatgga gacgtacctg ttcgttagcc aacagaatac cgatgattcc cgtcttcacc
    31621 tgcctgtttt gccttttcct ggcatcgttt cttgcggaaa tggcgcagac cgacatggga
    31681 tattttattc cttcgcgggt tttggtcggt ttaggagcgg tatgctttac gttgttctca
    31741 atcgtttcaa tattagaagc aggttctgct aaaaaataat tgcaacgtac cggataaaac
    31801 cagcgttgac catttgcgta acgctggttt ttcttaggca tcatgaaata acgcagcatt
    31861 aatgcatagt ggttaaggat aaaaaaacaa caaagttact gtttttttca acctgttcat
    31921 attttataaa gttctggcca ctgggtgagt tttcaatcac ctttccttcc accttatatt
    31981 aagcctggag ggtttcagtt cacgggctca ttagaaaata atcacaagat agccactatc
    32041 aaaattagct catttttaat acggctctaa taattttcat ctttaggtaa caggtcagga
    32101 cgacttcgct gcaatcaaat cctgcgcaca agcccacgca ctcgaccacg cccactggaa
    32161 gttatagccc cccagccagc cggtaacgtc catcacttcg ccgatgaagt acagcccagg
    32221 cactttgcgc gcttccatcg tccgtgaaga gagttcgttg gtgtccacgc cgccgagcgt
    32281 cacttcggca gtgcgatagc cttcagtgcc gttgggttgt acgcgccagt cggtcaatgt
    32341 gctaatcagt gcctgttggt cacgcacgtt gagctgtttt agcgaaacat ccgggatttg
    32401 cccgagttgc tgtaagcgtt caaccaaccg cttcggtaga tgaaccgcca gtgtgttttt
    32461 caggctctga ttcggatgtg cgttacgctg ctcattcagg aaggtttcga gatccacatc
    32521 cggtagcaga ttgatactga caaattcacc cggttgccag tagctggaaa tctgcaacac
    32581 cgccggtcca gacaagccgc gatgggtgaa gagtaagttc tcacggaaaa cgatgccgtt
    32641 ttcagcggta atcacggaag gcaccgccac gcccgccagc acctgtaact cttcgagcaa
    32701 cggtttatgc agagtgaatg gcaccagacc cgcgcgggtc ggcagcacgt tgaggccaaa
    32761 ttgttcggca atcttataac caaacggcga cgcgcccagc cccggcattg acagcccacc
    32821 ggtcgcgatg accagctttt cgcaaccgac agtcatgccg ttcagttcaa gcgtgaagcc
    32881 tgtttcatcc ttcgccacac tcagcacttc gctacgcaat ctgaaggtta cattgccctt
    32941 ctcgcactca tccaccagca tgtcgacaat ctgctgcgcg gagtcatcgc agaagagttg
    33001 tcctaacgtt ttctcgtgcc aggcgatgcc gtgtttattg accagatcaa tgaaatccca
    33061 ctgggtaaaa cgcgcgagtg cagacttaca aaaatgcgga ttctggctca gataagcgcc
    33121 tggttcgaca taaaggttgg taaagttgca gcgcccaccg ccagacataa ggattttgcg
    33181 ccctggtttt ttaccattat cgatcagcag aacccggcgt cctgcctgac ctgccagcgc
    33241 agaacagaac atacccgccg caccagcgcc tataataatg gcatcaaacc tttccacgtt
    33301 gcgctcctct tagaaaaaac gggcgtgaat tgtaaagatt cctcagtggt cgcaccagca
    33361 tcaatattac taaaaggaag tatttgcctg aattatataa gataattatt ttttgagtga
    33421 aatccataca ggaggcaaat caaaaaaagt ctatatttca ctttgcccgc gccgcgaaag
    33481 tcactgataa tgcgccgcgt tcatgtcctc aaaatggcgt aacgtcctat gctacatttg
    33541 tttgctggcc tggatttgca taccgggctg ttattattgc ttgcactggc ttttgtgctg
    33601 ttctacgaag ccatcaatgg tttccatgac acagccaacg ccgtggcaac cgttatctat
    33661 acccgcgcga tgcgttctca gctcgccgtg gttatggcgg cggtgttcaa ctttttgggt
    33721 gttttgctgg gtggtctgag tgttgcctat gccattgtgc atatgctgcc gacggatctg
    33781 ctgcttaata tgggatcgtc tcatggcctt gccatggtgt tctctatgtt gctggcggcg
    33841 attatctgga acctgggtac ctggtacttt ggtttacctg catccagctc tcatacgctg
    33901 attggcgcga tcatcgggat tggtttaacc aatgcgttga tgaccgggac gtcagtggtg
    33961 gatgcactca atatcccgaa agtattaagt attttcggtt ctctgatcgt ttcccctatt
    34021 gtcggcctgg tgtttgctgg cggtctgatt ttcttgctgc gtcgctactg gagcggcacc
    34081 aagaaacgcg cccgtatcca cctgacccca gcggagcgtg aaaagaaaga cggcaagaaa
    34141 aagccgccgt tctggacgcg tatcgcgctg atcctttccg ctatcggcgt ggcgttttcg
    34201 cacggcgcga acgatggtca gaaaggcatt ggtctggtta tgttggtatt gattggcgtc
    34261 gcgccagcag gcttcgtggt gaacatgaat gccactggct acgaaatcac ccgtacccgt
    34321 gatgccatca acaacgtcga agcttacttt gagcagcatc ctgcgctgct caaacaggct
    34381 accggtgctg atcagttagt accggctccg gaagctggcg caacgcaacc tgcggagttc
    34441 cactgccatc cgtcgaatac cattaacgcg ctcaaccgcc tgaaaggtat gttgaccacc
    34501 gatgtggaaa gctacgacaa gctgtcgctt gatcaacgta gccagatgcg ccgcattatg
    34561 ctgtgcgttt ctgacactat cgacaaagtg gtgaagatgc ctggcgtgag tgctgacgat
    34621 cagcgcctgt tgaagaaact gaagtccgac atgcttagca ccatcgagta tgcaccggtg
    34681 tggatcatca tggcggtcgc gctggcgtta ggtatcggta cgatgattgg ctggcgccgt
    34741 gtggcaacga ctatcggtga gaaaatcggt aagaaaggca tgacctacgc tcaggggatg
    34801 tctgcccaga tgacggcggc agtgtctatc ggcctggcga gttataccgg gatgccggtt
    34861 tccactactc acgtactctc ctcttctgtc gcggggacga tggtggtaga tggcggcggc
    34921 ttacagcgta aaaccgtaac cagtattctg atggcctggg tgtttaccct tccggctgcg
    34981 gtactgcttt ccggcggact gtactggctc tccttgcagt tcctgtaatc gtacacacca
    35041 aaacgagcgg gtcagtttac tggcccgctt tttttatgcg ttattattgc tgaagtgctt
    35101 aatgccaaat catcaatgca atcaggctga ccaccaccag accacacaat gcgctggtca
    35161 gaataaactg ccgacgcacc cgctcacagc ggcgaataaa ttcatcatcg tgatgatcgc
    35221 gataacgttg ggcatagata taccaaacga gacgcacctg tttgttgggt tggccatgtg
    35281 aggtaaaaaa gccccctcca tcaacatatt gatagagcaa tggatcgcag ttacgcagta
    35341 ccactaacaa cgcgcgtagt gatgagaaat agcgcgccat gttaacaatg caaacgacac
    35401 ataaagccca aaataatgcg acggtgctta tcatacctcc tccccggcga cctgcccgcg
    35461 gagttccacc ccggggctac cgctcccgat acgctgccaa tcagttaaca ccaggtcctg
    35521 gagaaaccgc ttttgtggtg accaacatcc gagcggctct atagatagtg taggagatca
    35581 ggttgttttt tttccagaag gttaaccact atcaatatat tcatgtcgaa aatttgttta
    35641 tctaacgggt aagcaaggcg gattgacgga tcatccgggt cgctataagg taaggatggt
    35701 cttaacactg aatctttacg gctgggttag ccccgcgcac gtagttcgca ggacgcgggt
    35761 gacgtaacgg cacaagaaac gctagctggc cagtcatcga caactttatg gaaggagtaa
    35821 cactatggct tataaacaca ttctcatcgc ggtcgatctc tccccggaaa gcaaagttct
    35881 ggtagagaaa gcagtctcta tggctcgccc ctacaatgcg aaagtttctc tgatccacgt
    35941 agatgtaaac tactctgacc tatacaccgg gcttattgat gtgaatctgg gtgatatgca
    36001 gaaacgcatc tctgaagaga cacatcatgc gctgaccgag ctttccacta atgcaggcta
    36061 cccaatcact gaaaccctga gcggcagcgg cgacctgggc caggttctgg tcgatgcaat
    36121 caagaaatac gatatggatc tggtggtttg tggtcaccac caggacttct ggagcaaact
    36181 aatgtcttcc gcacgtcagc tgatcaacac cgttcacgtt gatatgctga ttgttccgct
    36241 gcgcgacgaa gaagaataat catcccccca cgacgtgttc ctgaacgccc gcatatgcgg
    36301 gcgttttgct ttttggcgcg ccttgttacc tgatgcagcg taaacccctt atctggccta
    36361 cgatctgcgt acgcaatcaa aatctcctgc caatacaaca tttaacacca tcatattttc
    36421 catcattagt gtgatcagct ggttattttc tgttgtaata gtgtattaat ctattcaccg
    36481 catcaatatt aagaatctct gacagatgta aactttttcg cgcgttatcc cttacgcgtt
    36541 catacttttc aggatggtat tggaaggtta ataaatatga atacaacaac acccatgggg
    36601 atgctgcagc aacctcgccc atttttcatg atcttttttg tcgagttatg ggagcgattc
    36661 ggctactacg gcgtgcaggg cgtactggcg gttttcttcg ttaaacagct tggattctcg
    36721 caagagcagg cttttgtcac ttttggtgct tttgctgcgc tggtctatgg cctcatttcc
    36781 attggcggct atgtcggcga ccacctgctg gggaccaaac gcaccattgt tctcggagca
    36841 cttgtgctgg cgattggcta cttcatgacc ggcatgtcgc tacttaagcc tgacctgatt
    36901 ttcatcgccc tggggactat cgctgtcggt aacggcctgt ttaaagctaa cccagccagc
    36961 ttgctttcga agtgctatcc gccgaaagat ccgcggcttg atggcgcatt caccctgttc
    37021 tatatgtcga tcaatatcgg ctcgttgata gcgttatcgc tggcccctgt gatcgctgat
    37081 agattcggtt attcagtcac ctacaacctg tgcggggcgg ggttaattat cgcattactg
    37141 gtttacatcg cctgtcgtgg aatggtgaaa gacattggtt ctgaacccga cttccggcca
    37201 atgagcttca gcaaactgtt gtacgtgtta cttggcagcg tggtgatgat cttcgtctgt
    37261 gcatggctga tgcacaacgt agaagtcgcc aatctggtgc tgattgttct ctccatcgtc
    37321 gtcaccatca tcttctttcg tcaggcattc aagctggata aaaccgggcg caataaaatg
    37381 tttgtcgcct ttgtcctgat gctcgaagcg gtggtgtttt acattctcta cgcccagatg
    37441 ccaacgtcgc tgaacttctt tgccatcaac aacgtgcatc atgaaattct cggcttttcc
    37501 atcaacccgg tcagcttcca ggcgcttaac ccgttctggg tggtactcgc cagcccaata
    37561 ctggcaggca tttacacgca tctgggtaac aaaggcaaag acctctcgat gccgatgaaa
    37621 tttactctcg gcatgtttat gtgctcactg ggctttttga cggcggcagc tgcgggaatg
    37681 tggtttgctg atgcacaagg actgacatcg ccgtggttta tcgtgctggt ttacttgttc
    37741 cagagcctgg gtgaattgtt tattagcgcc ctgggtctgg cgatgattgc tgccctggtg
    37801 ccgcagcatt tgatgggctt tattctcggg atgtggttcc tgacgcaggc tgccgcgttc
    37861 ttgctgggcg gctatgtggc aacatttacc gcggtgccgg acaacattac cgatccgctt
    37921 gagacgttgc ccgtctatac caacgtgttt ggtaagattg gtctggtcac gctgggcgtt
    37981 gcagtagtga tgctgttgat ggtgccgtgg ctgaaacgca tgattgcgac gccggaaagc
    38041 cattaattat tcttgcagaa agcagggtag cgttatcgct accctgcttt acttttacac
    38101 cggcgttccg gcataaatat caaacctatg ccctttagtg actaccgcgt ttggcgtggc
    38161 gacattcgcc agcggtggcg cgtagtccgg gcgctttacc accacgcgtt tggtcgccag
    38221 cagccgcgca ggctccagca atccatcggc atcaagatcc ggccccacca gcgactgaaa
    38281 gacacgcatc tcttttttca ccagcgcgct tttctgctta tgcgggaaca tcgggtcgag
    38341 ataaaccacc tgcgggcgcg gggtaatatc agtcagcgcc gtcaggctgg aggcgtgaat
    38401 taactgcaac cgctcctgca accagccgcc gatttccgca tccgcataac cacgcgccag
    38461 gccgtcgtcg agcaaggcgg caacgactgg attacgctcc agcatccgca cgcggcaacc
    38521 gactgaagcc agcacaaagg catcgcgccc cagccctgcg gtggcgtcca ccacatccgg
    38581 caaataatcg cctttaatgc ccaccgcttt cgccaccgcc tcaccgcgac caccgccgaa
    38641 tttgcgtcgg tgcgccatcg ctccgccaac aaaatcaaca aagatgccgc caagttttgg
    38701 ctcatcacgc ttgcgcaatt ccagatgttc cggcgttaac accagcgcca tcaggttgtc
    38761 ttcatcgtgc tccagccccc agcgggccgc cagaacagat aaggctccgt ctccggtgcc
    38821 tgtttcatca attaagcaaa tctgcacaaa aactaacctt tgatcccgta atgctccagc
    38881 atcgcatcca gctgcggctc acgaccacgg aagcgtttga acagatccat cggctcctct
    38941 gaaccgccac ggctcagaat gttgtcgagg aacgactgcc cggtttcacg gttgaaaatg
    39001 ccctcttcct caaagcgcga gaaagcatct gccgccagta cgtcagccca caggtagctg
    39061 tagtaacctg cggcataacc accggcgaaa atatggctga aagcgtgcgg gaaacggccc
    39121 caggatggag acggtactac ggcaaccagt ttcttgattt ctgccagagt ttcgaggatt
    39181 ttcgctccct gatccgggcg gaactcggca tggaggcgga aatcgaacag gccgaactcc
    39241 agctggcgca gaataaacag cgccgcctgg tagttcttcg ccgccagcat tttatccagc
    39301 aactctttcg gcagcggttc gccggtttcg tagtgaccag agataaacgc cagcgcctca
    39361 ggctcccagc accagttttc cataaactga ctcggcagtt cgaccgcatc ccacggcacc
    39421 ccactgatcc cggatacacc ggcggtttcg atgcgggtca gcatatgatg cagaccatga
    39481 ccgaactcgt ggaacagggt gatcacttcg tcatgggtaa acagcgccgg tttaccattt
    39541 accgggcggt taaagttaca ggtcagatac gcgaccggtt tttgcagaga accgtcagct
    39601 ttgcgcatct ggcctacgca gtcatccatc cacgccccgc cgcgcttgtt ttcacgggca
    39661 tacaggtcga ggtagaagct accgcgcagt tcgttgttct cgtcatacag ttcgaagaaa
    39721 cgtacatccg ggtgccagac atcaacatct ttacgctctt tagcggtgat gccgtaaata
    39781 cgtttcacca cttcaaacaa accgttaacc gctttgtttt ccgggaagta cggacgcagc
    39841 tgttcgtcac tgatgctgta gaggtgctgt ttttgttttt cgctgtagta cgcgatatcc
    39901 cacggctgca actcatcgac gccaaattcg gctttggcaa aggcgcgcag ttgtgccagc
    39961 tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg ttaagaaatc cagcacctgc
    40021 tgcgggtttt ctgccatttt agtggcaagg gatttaaagg cgtagttttc aaagcccagc
    40081 agttgcgcca gttcgtgacg cagcgcgagg atctcttcca tcaccttgct gttgtcccac
    40141 ttaccggcgt tcggaccttg atcggaggcg cgggtgctgt aagcgcgata catctcttca
    40201 cgcagagcct ggttgtcgca gtaggtcatt accggcaggt agcttgggat atccagcgtc
    40261 agcaggtagc cttcaagttc tttcgcttca gcctgggctt ttgccgcagc cagcgcgctt
    40321 tctggcatcc ccgccagttc cgcttcgtcg gtaacgagtt tggtccagcc cattgtcgca
    40381 tcgaggacgt tgttgctgta ctggttgccc agttcagaaa gacgggtagc aatttcgccg
    40441 taacgctgct gtttctcttt cggcagacct atgccagaga gttcaaaatc acgcagggcg
    40501 ttatcaaccg ctttcttctg cgccgtgttc agcgtggcgt aatgatcgcc atcgcgcagg
    40561 tcgcggtacg ctttatacag cccttcatgt tgccctaccc aggtgctgta ttccgacagc
    40621 agcggcaggg tttgttcgta cgcttcacgc agttccgggc tatttttcac cgagttcagg
    40681 tggctgaccg gggagaagat acgccccaac acatcgtcca cttccgccaa cggctggcag
    40741 agattttccc aggtgtacgg tgccccttgc gctactacgc gctccacgtt ttcgcggcag
    40801 tcgttcaatg ccttagtcac ggctggaacg acatgttccg ggagaatttt agaaaacgga
    40861 ggcaattcaa agggagtcag taacggattc gtcattcgcg cagtcctggt taaagaggtt
    40921 aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc atggggttaa gtgtagtgaa
    40981 tttcaatgag aaacgttacg ctttcgcggc ggcggcctct tttcggtata ctgtcctgat
    41041 acgcttttgt gcgccccgaa tacgggccga tttttactta ccggaacact tttacccatg
    41101 ctcagttatc gccacagctt tcacgctggc aaccacgccg acgtccttaa acataccgtt
    41161 cagagcctga tcatcgagtc gctgaaagag aaagataaac cgtttctcta tctcgacacc
    41221 cacgcagggg ccgggcgtta tcagttaggc agcgaacatg ccgagcgtac cggcgaatat
    41281 ctcgaaggca tcgcccgtat ctggcagcag gacgatttgc ccgcagaact ggaggcgtac
    41341 atcaatgtgg taaaacactt caaccgtagc ggtcagttgc gttactaccc cggttcgccg
    41401 ttgattgctc gccagctact gcgcgaacaa gacagcctgc aactgaccga actgcacccg
    41461 agcgattacc cgttgctgcg ttctgaattt cagaaagaca gccgtgcgcg cgtcgaaaaa
    41521 gccgatggtt tccagcagct taaggccaaa ctgccgccgg tttcccgccg tggtttaatc
    41581 cttatcgacc cgccgtatga aatgaaaacc gactatcagg cggtggtcag cgggatagca
    41641 gaaggttaca aacgtttcgc cactggtact tacgcactgt ggtatccggt ggtgctgcgt
    41701 cagcaaatta agcgcatgat ccacgatctg gaagcgaccg gtattcgcaa aattctgcaa
    41761 attgaactgg cggtactgcc agacagcgat cgccgtggca tgaccgcttc cggcatgatt
    41821 gtgattaacc cgccgtggaa actggaacaa cagatgaata acgtgctgcc gtggctgcac
    41881 agcaaactgg ttccggcagg caccgggcac gccaccgtaa gctggatcgt gccggagtaa
    41941 ttgcagccat tgctggcacc tattacgtct cgcgctacaa tcgcggtaat caacgataag
    42001 gacactctgt catgactaaa cactatgatt acatcgccat cggcggcggc agcggcggta
    42061 tagcctccat caaccgcgcg gctatgtacg gccagaaatg cgcactgatt gaagccaaag
    42121 agctgggcgg cacctgcgtg aacgtcggct gtgtgccgaa aaaagtgatg tggcacgcgg
    42181 cgcaaatccg tgaagcgatc catatgtacg gcccggatta tggttttgat accactatca
    42241 ataaattcaa ctgggaaacg ttgatcgcca gccgtaccgc ctatatcgac cgtattcata
    42301 cttcctatga aaacgtgctc ggtaaaaata acgttgatgt aatcaaaggc tttgcccgct
    42361 tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat cacggccgat catattctga
    42421 tcgccacagg cggtcgtccg agccacccgg atattccggg cgtggaatac ggtattgatt
    42481 ctgatggctt cttcgccctt cctgctttgc cagagcgcgt ggcggttgtt ggcgcgggtt
    42541 acatcgccgt tgagctggcg ggcgtgatta acggcctcgg cgcgaaaacg catctgtttg
    42601 tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat ttccgaaacg ctggtcgaag
    42661 tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat cccgaaagcg gtagtgaaaa
    42721 atgccgatgg tagcctgacg ctggagctgg aagatggtcg cagtgaaacg gtggattgcc
    42781 tgatttgggc gattggtcgc gagcctgcca atgacaacat caacctggaa gccgctggcg
    42841 ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata tcaaaacacc aatattgaag
    42901 gtatttacgc ggtgggcgat aacacgggtg cagtggagct gacaccggtg gcagttgcag
    42961 cgggtcgccg tctctctgaa cgcctgttta ataacaagcc ggatgagcat ctggattaca
    43021 gcaacattcc gaccgtggtc ttcagccatc cgccgattgg tactgttggt ttaacggaac
    43081 cgcaggcgcg cgagcagtat ggcgacgatc aggtgaaagt gtataaatcc tctttcaccg
    43141 cgatgtatac cgccgtcacc actcaccgcc agccgtgccg catgaagctg gtgtgcgttg
    43201 gatcggaaga gaagattgtc ggtattcacg gcattggctt tggtatggac gaaatgttgc
    43261 agggcttcgc ggtggcgctg aagatgggtg caaccaaaaa agacttcgac aataccgtcg
    43321 ccattcaccc aacggcggca gaagagttcg tgacaatgcg ttaaatgtta aagggctaag
    43381 agtagtgtgc tcttagccct taattacgtt tccgctatca gttcagaagc tgaagcagaa
    43441 agcggatcag ttccagcagc gcaattaacg ccccaagaac gatgattgct ttatcaatca
    43501 cccgttttct ccatgcgatg gagtgagaat gcatccgctt actcatccac tgcctgtcac
    43561 ggcgcatgcc ctattgttag ataatggcat tctcactccg gccagagcat cagttaacgg
    43621 caccacccgt acttctgacc aggactttga aagcgtttat gcgcattacc agagtgaaaa
    43681 tgcctcagag ctaactggat aatcatacag tacattcagg ttataaaacc agcatgtcct
    43741 tgcaatagtt tcagtatggt attagcattg atgcgttaga taatggcatt ctcactccag
    43801 ccagtgccac caactcaggg ctggaaagta aaaaaccgac gcaaagtcgg tttttttacg
    43861 tcctgattca gacctccttt caaatgaata gccaactcaa aattcacacc tattaccttc
    43921 ctctgcactt acacattcgt taagtcatat atgtttttga cttatccgct tcgaagagag
    43981 acactacctg caacaatcag gagcgcaata tgtcatttct gttacccatc caattgttca
    44041 aaattcttgc tgatgaaacc cgtctgggca tcgttttact gctcagcgaa ctgggagagt
    44101 tatgcgtctg cgatctctgc actgctctcg accagtcgca gcccaagatc tcccgccacc
    44161 tggcattgct gcgtgaaagc gggctattgc tggaccgcaa gcaaggtaag tgggttcatt
    44221 accgcttatc accgcatatt ccagcatggg cggcgaaaat tattgatgag gcctggcgat
    44281 gtgaacagga aaaggttcag gcgattgtcc gcaacctggc tcgacaaaac tgttccgggg
    44341 acagtaagaa catttgcagt taaaaattta gctaaacaca tatgaatttt cagatgtgtt
    44401 ttatccggga ggcattatgt tactggcagg cgctatcttt gtcctgacca tcgtattggt
    44461 tatctggcag ccgaaaggtt taggcatcgg ctggagtgca acgctcggcg cagtactggc
    44521 gttagttacg ggcgtggtcc atccgggtga tattccggtg gtgtggaata tcgtctggaa
    44581 cgcgacggct gcgtttatcg ccgtcattat catcagcctg ctgctggatg agtccggctt
    44641 ttttgaatgg gcggcgctgc acgtctcacg ctggggtaat ggtcgtggtc gcttgctgtt
    44701 tacctggatt gtcctgctcg gtgctgccgt tgccgccctg tttgccaatg atggcgcggc
    44761 gcttattttg acaccgattg tcatcgccat gctgctggct ttagggttca gtaaaggcac
    44821 tacgctggcg ttcgtgatgg cggccggatt cattgccgat accgccagcc tgccgcttat
    44881 tgtctccaac ctggtgaata tcgtttccgc tgatttcttt ggcctcggct ttcgcgaata
    44941 cgcctcggtg atggtgccgg tggatatcgc cgcgattgtt gccacgctgg tgatgttaca
    45001 tctctatttt cgcaaagata ttccgcagaa ctacgatatg gcgctgctga aatctcccgc
    45061 agaagcgatc aaagatcctg ctacgttcaa aactggctgg gttgttttac tgcttctgct
    45121 ggtgggattt ttcgtcctgg aaccgctcgg cattccggtg agcgccattg cagctgtggg
    45181 cgcgctgata ttatttgtcg tcgctaaacg cggtcatgcg attaatacgg gtaaagtcct
    45241 gcgcggtgcc ccctggcaga ttgtcatctt ctcgctcggc atgtatctgg tggtttatgg
    45301 cctgcgcaat gccggattaa cggaatatct ttctggcgta ctcaacgtgc tggcggataa
    45361 cggcctgtgg gccgcgacgc tcggcaccgg attcctcacc gccttcctct cttctattat
    45421 gaacaatatg ccgacggtac tggttggcgc gttgtccatt gatggcagca cggcatctgg
    45481 cgttatcaaa gaagcgatgg tttatgccaa tgtgattggc tgcgatttgg gaccgaaaat
    45541 taccccaatt ggtagcctgg ctacgctact ctggctgcac gtactttcgc agaagaatat
    45601 gactatcagc tggggatatt acttccgtac agggattatc atgaccctgc cggtgctttt
    45661 tgtgacgctg gctgcgctgg cgctacgtct ctctttcact ttgtaatgag atattgatat
    45721 gagcaacatt accatttatc acaacccggc ctgcggtacg tcgcgtaata cgctggagat
    45781 gatccgcaac agcggtacag aaccgaccat tatctattat ctggaaacac cgccaacgcg
    45841 cgatgagctg gtcaaactca ttgccgatat ggggatttcc gtacgcgcgc tgttgcgtaa
    45901 aaacgtcgag ccgtatgagg aactgggcct tgcagaagat aaatttactg acgatcggtt
    45961 aatcgacttt atgcttcagc acccgattct gattaatcgc ccgattgtgg tgacgccgct
    46021 gggaactcgc ctgtgccgcc cttcagaagt ggtgctggaa attctgccag atgcgcaaaa
    46081 aggcgcattc tccaaggaag atggcgagaa agtggttgat gaagcgggta agcggttgaa
    46141 ataaagcggc gatatccact gcccatagtt ggtaaagtgc gctttgttta tgtcggatgc
    46201 gacgctaacg cgtcttatcc gacctacaaa accgtgcatg gcatgaaatc tgtagcgcgt
    46261 agcgcatcag gcaattttgc tatttacgca gcactcctcg cgtaagaatt caattatcgc
    46321 ttccagcatt gcgtattgcg aaacacacat cagcgtccga ctctcacggt tttgtttaac
    46381 cagccctacc gataccagtg ctgaaatatg gtgactgagt gtagagccgg gtatcccaag
    46441 ctgcttttgc aactcaccga ccggcaaacc ttgctccccc gctttgacca ggtgtttgaa
    46501 tatgaacaaa cgggtcgggt gacctagctc tttcagcgct ttagcaactt cttccaactg
    46561 catatgacac cccctttgtc tggttgatat ttcgataata ccagaaatat cttgatctga
    46621 atttcatttc gattattctg gaaatataga tttatccaat gaggttacgt atgagtagtt
    46681 ggcttgcgat gctgcaagat gccgcagaga tgtttgtgtt tctcgccgtc gagctttcgt
    46741 tgctgtttat agtgattagt gccggtgtca gcctgataag acaaaaggtg ccagaccata
    46801 aaatccagca gatgatgggg gccagaaaag ggagaggcta tctcctggct gctctgttgg
    46861 gagccgttac cccgttctgt agttgctcga caatacccat gttacgtgga ttgttgtcag
    46921 cgaaagccgg gtttggtccg actctcactt ttttatttgt ttcaccatta cttaatccca
    46981 ttatcgtcgg actaatgtgg atgacctttg gttggaaagt caccttgttg tacgcgatta
    47041 tcgccgccgg cgtctccgtt cttgccagta ttatcctaga ttctctgggg tttgaacgtc
    47101 atatcattgc cagtaaaagc tcatcagcaa attgttgtgc tctagcaaaa acttcgccgg
    47161 gaacgacata tacgcccata aaagtgagtt gttgtagtcc agcggctaaa gccattgaga
    47221 accccgtagt tacctgttgc agtaccaaag ctgtagtaag tattaatccc ataaaactag
    47281 ccaccaaaga tgcgttgcaa cagtttaaag atgtactgcc atatcttttg ttaggagtat
    47341 taataggatc ttttatttat ggctttattc cctcagcgtg gattgccgct catgcaggag
    47401 cagataatcc cttcgccatc ccattaagcg ccgttgtcgg tattccgctg tatatccggg
    47461 ctgaggcagt tatccctctg gcatctgttc tgatgacaaa aggaatgggt ctgggagcat
    47521 taatggcatt aatcatcggc agtgccggcg caagcctgac ggaagtgata ttacttaaat
    47581 caatgttcag aatgccgatg atagctgcat tcctgacggt tatattaggt atggctatct
    47641 tgatgggcta tttgactcaa ttgctatttt aaatagatgc cagcagtaaa ctataagatg
    47701 catagttagt gccatatcct tctaagtaga aaatcataga caggataatc cttaaaaatc
    47761 acaaatgatt taactaatgc attgagaatg atgacggctc ggtcaacaag tcatcaacag
    47821 acaataacca cgtaatcatg cttctctgac attttatcgg agaagcaatg actctttgtc
    47881 tcagtaccct cctccttttt aaaaggcaga tttcagtcgc taatacaaca catttttaaa
    47941 tacactttaa cctggttcat tattacacat ctaaaacatc acaaaaatat tacttcacac
    48001 actatcggag aacataataa ctttttaaaa tgagcgcttt ttgatagttg ttaacgtttt
    48061 cctttacgat actatcactg ccttttttta tacacaaata ttaccgggtg gaaatgaaag
    48121 ggaatttaaa ggtaattata tcaggaagat ataacgcatt atatttattg tgtgtagaag
    48181 aaaatccatt cctgttctaa ttattcaatt taaaaacctt agcataaaac agatgatgaa
    48241 taaggaatgt tatgtcaatt gactttaccc caggtataat aaatacatat cacggcgata
    48301 tttataactg cacaacaaat accgataatg ttaaaacgcc agacacaccg aagtggcctt
    48361 gtgataattg ggaagaacaa caacccatta attccatatt ctctggagaa ggatacaacc
    48421 ctgaacaatt tgatttagcg caacaacaac tccaacaaat taatgcatgc cacacgaaca
    48481 ccacatatac aaatgcagac tactcaaaag ttgtggttca acttgtaagt cttattaata
    48541 acattgaaac gataagttcg acaccactta cacaacagac acaatcaata cttaatcaga
    48601 ttaataacat tagatatgaa aaaaacaaaa gtgctgaatg tcgaatcatt gttgtcgcta
    48661 atcctaaacc cgacaaggca atcataacta aaattagcgt agaagaggga atacccataa
    48721 cattcagtgt ccaaaccatg ttttcagata ctaattttat tgctgaacaa cgagctgact
    48781 taccaactaa tataaaagat attcaaagcc tgtatcaaaa aatgacgaag ttatatattg
    48841 agcacagtga aaataaaaac aggatgaaag tctttgccgg aaccaacttt attgatttca
    48901 atatgacagg acagaacctg tccgggttcg ttctaacctt gtcaaggttt tatttcgaag
    48961 atctacttaa tataaatttc actgacgcaa atttaggtga cactattttt tacataaaga
    49021 acaccctacc cccaaattat ataaagatgg acaatatctt gacaaacaaa tcgaaggttt
    49081 attttcaaca ttattaacca ttaatgacaa ctttttacga gcaaaagcgg aaattgcttc
    49141 gacaataatt aaatttttag aggctaggat aaccaatctt tcatacaatg acatattaac
    49201 atatcagcaa gaattccaaa aacaatgtta taagcaagtt aaagcctttg cgacactctc
    49261 tcgatacaat aaaattcaaa catgggcaga aatgtctgaa tatcagttcg aagtatttca
    49321 atacgagacg cttaacccca aaaaaatgtc acacacgcct tatttaaaga ggccacttcc
    49381 taatgaaaaa gatataaatt atggggtgga aatagaaata ccatctggca aaagaattcg
    49441 cttatcaaat cattatcaga atataatacc ataatattga ttattagcac tttataatta
    49501 ttgaataaaa atagatttta tgtaatttta aaacaaaggg ttatattatt gggcgatgga
    49561 tactcatacc acgccccaaa ataacggaat tgaactaaac agaagggata taaacatcag
    49621 acaggtttac gttactatca ggcatatcac ctcagaatca gatgaaaact ataaagaaat
    49681 atctattatg gttttaatat ttgttgataa ggatagtaac atgaacatga caaaaggtgc
    49741 actcatcctc agcctttcat ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa
    49801 aggcaataac caacctgata ttcaaaaaag ttttgttgct gttcataacc agccggggtt
    49861 atatgttggt caacaagcgc gctttggtgg gaaggttatc aacgttatca atggcaaaac
    49921 ggatacgttg ttagaaatcg ctgtattacc gttggatagc tatgcgaagc ctgatattga
    49981 agccaactat cagggccgac tgctcgccag acaaagcggc ttccttgatc cagtgaacta
    50041 tcgtaatcac tttgttacca tcctcggtac cattcagggt gaacaaccag gctttatcaa
    50101 taaagtcccg tataacttcc tggaagtgaa tatgcagggc atccaggtgt ggcatttgag
    50161 agaagtggtt aataccacct ataacctgtg ggattacggc tatggtgcat tctggccgga
    50221 accgggctgg ggtgcgcctt actacaccaa tgcggtgagt caggtaacac ctgagctggt
    50281 caaataacac caccgaaaga tgcagatgct ttctcagcat ctgcatcatg tattacataa
    50341 aattaatata cagtaagcta actattatta ttataagccc tgtcctgtta attacctttg
    50401 gcaaactgat tataaagtta atgtccgcac caggagtcgg ttatgtttct tataattacc
    50461 agggatacga tgttcttcac cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat
    50521 atacagaacg aagaagagat cgacgtaatg ttgcatcaga atgccttcgt cattattgat
    50581 acattaatga ataatgtatt tcattctaat tttctcactc aaattgaacg attaaaacct
    50641 gtccatgtga ttattttctc cccctttaat attaaacgct gcctggggaa agtgccggtg
    50701 acctttgttc cgcggactat cactatcatt gattttgtcg cactcatcaa tggcagttac
    50761 tgctctgtgc ctgaagcggc tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc
    50821 attgcgaatc aaatgacaac ggaagatatt ctggagaaac tgaaaatatc gctaaaaacg
    50881 ttctactgcc ataaacacaa tatcatgatg atcctcaatc ttaagcggat caatgagctg
    50941 gtacgccatc agcatattga ttatctggtg tgaatttcag gcttacggtg agtctggcta
    51001 cgctgccaca cagattagct aattgaaacg cctttcaccc ctgccatacc ttttaataat
    51061 cgcaacaggt cttctatcga cgttgtggca tgaagctgga tatcaatggc gaccacttca
    51121 tgatcttcat tttcctgcaa cgaaactaaa tccgttttta ttttttgctg cttaaaccag
    51181 tcgagcattg ataccgtatt accgttcact aatgtcagct gtaaatggta atttttgttc
    51241 atcaggcgga aggttaattg atggaagact tccagcacca acaaggtcat cactgaacca
    51301 taaataccca gttcgtacat accgctgcca ataaccatac ctatggcggc ggtcacccag
    51361 atatccgctg ccgtcgtcag acctacaata ttttggttac gaacgagaat gttacctgca
    51421 ccgataaacc cgacgcccgt caccacctga gcagcaatac ggctggggtc gagtccgacg
    51481 tgatctaaag acagcacgtc agcaaaacca tatttcgaaa caatcataaa cagggcgctt
    51541 cccatgccaa ttaatacatg tgtgcgtaac cctgctcctt tgccgcgcat ttgcctttcc
    51601 atgccaatag cgccacaggc aattgccgcg agtataagac gtataataaa ttctgctgtc
    51661 atataaaaat gcaaaaagga gcagcaagat ggctcaactt gctactcctt tttacttgta
    51721 cctcattaat tcggcaagtc attagatgct tgatttttga aggtatacaa atttttctgc
    51781 gggtttttct tacagtattc gatcacttta ggaatttgag tgagatcggt ttcatttaaa
    51841 gtaacggtat cgccaccttt atatactgtt tcttcatgca gcatccacca tgcaaccggg
    51901 gtcattgctt ttggattcag atcaataaat tcctggcagg tcatatcttt agcggattca
    51961 ttggctgcca gcgcagattg tgcgttcacc agtgacaaag ccgctacagc gcccataaaa
    52021 ataaacgctt tacggagaga tgaaatattc attttgtaac ccattcaata tagagattat
    52081 atatgaacag gaggaatagc tgcttttaaa gaagatattc cggcctgttc aatatgcaag
    52141 gaagtacgat gtaacgcatt cccggaatta catatctttc ttaattttgt cccattcgcc
    52201 tttaacttta tctttaaagt tggcttgttt atcctgagta caagcctgaa cgatcgctgg
    52261 ggttacggtt gcaataccct gaacatctaa aaccgcatct tctggtttat ctttgttgtt
    52321 cagcgcttca gcaaaaccaa ctgcagttgg ctggaaggat tcgtccacag ccaggaaatc
    52381 ttcacaggtc caggagttga ccggtttttt gttatcagct gctttttgcg catccgctgc
    52441 attgctcaca actggcagaa gaagcagacc accaagaata acgcctaata cttttttcat
    52501 cgtaatatcc tcaactataa agtgaaagag ccgtcacgaa tcaatttcga cacttaggtt
    52561 ataacctggt tttctgtata tgtcatgttg atggaaaata tcaaaatcag atatttttat
    52621 ttcaatacaa tgagttacag atgcatcaga tactgcaatt aggaaatttt tattaaatcg
    52681 actgcattct tagacgcgtt tttggcatag attgatagca ggggattttc ttcttaattt
    52741 tatagggtgg ttctatgtta tatatagata aggcaacaat tttgaagttt gatctggaga
    52801 tgctgaaaaa gcatcgcaaa gcaatccagt ttattgccgt gctgctgttt atcgtcgggt
    52861 tgctgtgtat cagtttcccg ttcgtctctg gcgatatttt aagcacagta gtgggtgcat
    52921 tattaatctg ctcgggtatt gcgcttattg tcgggttatt cagcaaccgc agtcataatt
    52981 tctggccggt attatccggt ttcctcgtcg cagtcgccta tttattgatc ggctatttct
    53041 tcatccgcgc accggagctg ggcatttttg ccattgcggc atttattgcc ggtttgttct
    53101 gtgttgcagg ggttattcgc ctgatgagtt ggtatcgtca gcgttcaatg aaaggcagct
    53161 ggctacagct cgttattggt gtgctggata tcgtcattgc ctggatattc cttggtgcaa
    53221 cgccgatggt gtctgtaacg ctggtttcca cactggtggg aattgaactg atatttagcg
    53281 ccgccagcct gttcagcttc gccagtttgt tcgttaagca gcaataatta tcccggttgt
    53341 cacccggatc atagtcactt gatgtgacta tgatccgatt aatactctct ccgctacgca
    53401 gtgttgtaga tcaattgcgc actatcattg aaataattac ctgctagtga tcatttcaac
    53461 ctactgaatt tcatctaatt tttttcactc catggcaaat tagccatttc aaacattatc
    53521 atggctgata ttttccgtag tcaggtttaa tgttttaaaa gtgctgtggg aaagtgaaca
    53581 aagagttccg taagcgttga tgctatgggc ggttaaataa gtaatccggg ttcatttttt
    53641 tgcaactggc gttgattaca ttgcataaat atccgtgtct ccagacgcta tataaaaacc
    53701 tgaagacatg aatgcgttat ttactcaggt aatttcaatg cgttaaaaga aagctggcaa
    53761 tccaattgcc agcttaagtc gaaacaagga gactcgatat ttaaatcgga ttacatttta
    53821 actttagtaa tattcttcag agatcacaaa ctggttattg ataacttatt cttgggcagt
    53881 aatccgcaaa cgttaacttt ttgtttgata tttacaaatt gataacaacc aggaatctta
    53941 cttaggatca atatatggag tgcgtgatgg ataaatctga agtattgatt agtgttaata
    54001 gacgtattag ttcacgaagg gcaaagttct tataggcgtt tactatattg aacaacgatt
    54061 cggacaagga tgtaaataat gaaaaggatg acatgttcga aacgataacg gctaaggagc
    54121 aagttatgat ttttctcatg acgaaagatt cttttctttt acagggcttt tggcagttga
    54181 aagataatca cgaaatgata aaaatcaatt ccctgtcaga gatcaaaaaa gtaggcaata
    54241 aacccttcaa ggttatcatt gatacctatc acaatcatat ccttgatgaa gaagcgatta
    54301 aatttctgga gaaattagat gccgagagaa ttattgtttt ggcaccttat cacatcagta
    54361 aactaaaagc taaagcgcct atttattttg ttagccgcaa agaaagtatc aaaaatcttc
    54421 ttgagattac ttatggtaaa cacttgcccc ataagaattc acaattatgt ttttcacata
    54481 atcagttcaa aattatgcaa ctgattctga aaaataaaaa tgaaagcaat atcacgtcga
    54541 cgctcaatat ttcgcaacaa acattaaaga ttcagaaatt caacattatg tacaagctga
    54601 aactaagacg tatgagcgac atcgtcaccc tgggtatcac atcttatttt tagtcaggac
    54661 ataagcaact gaaattgatg gctggcatga cgagggatgc agatgctgat ttcattaccc
    54721 ccggtgatta ctaaaggaga ggctaaaacg actttattcc cctggtatgt gtatccacca
    54781 gtagaaccct tcgttgcccg aatgctggca ggaactgttg gcagaacggc aacatttttt
    54841 ttgtcgttga cctcaccatg tcgatcactg tgcctgtatc ccaccttact ggctgacaac
    54901 cccactatgc cgctggtctg taaatccctc atatctctcc tcgcgcgcaa tttaaagaac
    54961 cgttatttct caagaatttt cagggactaa aatgaacaga agaagaaagc tgttaatacc
    55021 gttgtttttc tgcggcgcga tgctcaccgc ctgcgatgac aaatcggcgg aaaacgccgc
    55081 cgccatgacg cctgaggtcg gtgtcgtcac actctccccc ggttcggtca atgtgttgag
    55141 cgaattgccc ggtagaaccg ttccttatga agttgccgag atacgtcccc aggtgggcgg
    55201 tattatcatt aaacgcaact ttatcgaagg cgataaagtg aaccagggcg attcgctgta
    55261 tcagattgat cctgcacctt tacaggccga gctaaactcc gccaaaggct cgctggcgaa
    55321 agcgctctct accgccagca atgcccgcat cacctttaac cgccaggcat cgttgctgaa
    55381 gaccaactac gttagccgtc aggattacga caccgcgcgc acccagttga atgaagcaga
    55441 agccaatgtc accgtcgcca aagcggctgt tgaacaggcg acgatcaatc tgcaatacgc
    55501 gaatgtcacc tcgccgatta cgggcgtcag cgggaaatcg tcggtgaccg tcggcgcact
    55561 cgttaccgct aatcaggcag attcgctggt taccgtacaa cgtctggacc caatttatgt
    55621 cgatctcacg cagtcggtgc aggatttctt acgcatgaaa gaagaggtcg ccagtgggca
    55681 aatcaaacag gttcagggca gtacgccagt acagctcaat ctggaaaatg gtaaacgcta
    55741 cagccagacc ggcacgctga aattctccga cccgacggtg gatgaaacca cgggctccgt
    55801 gacgttacgg gcgattttcc ccaacccaaa tggtgacttg ctgcctggca tgtacgtcac
    55861 ggcattagtg gatgaaggta gccgccagaa tgtattactg gtgccgcagg aaggcgtcac
    55921 ccacaacgcc cagggtaaag caacggcgct cattctggat aaagacgatg ttgtgcagct
    55981 acgcgaaatt gaagccagca aagccatcgg cgaccagtgg gttgtcacct ctggcttgca
    56041 ggctggcgat cgggtgatcg tttccggttt gcaacgcatt cgtccgggta tcaaagcacg
    56101 agcaatttcc tccagccagg aaaacgccag caccgaatcg aaacaataac gttgcaggct
    56161 taaggggact ttcatggcta actattttat tgatcgcccg gtttttgcct gggtacttgc
    56221 cattattatg atgcttgcag gtggtctggc gatcatgaac ttaccggttg cgcagtatcc
    56281 gcagattgcg ccaccgacca ttaccgtcag cgctacctat ccaggtgccg atgcgcaaac
    56341 ggtagaagac tcggtcactc aggtgattga gcaaaatatg aatgggcttg atggcctgat
    56401 gtacatgtct tcaaccagtg atgcggcggg taatgcctct atcactctga ccttcgagac
    56461 tgggacatct cctgatatcg cacaggttca ggtgcaaaat aaactgcaac tcgctatgcc
    56521 ttcattacct gaagcggtgc agcagcaggg gattagcgtt gataagtcga gcagtaatat
    56581 tctgatggta gcggcgttta tttctgataa cggcagcctc aaccagtacg atatcgcgga
    56641 ctatgtagcg tctaatatca aagacccgct aagccgtacc gcgggcgttg gtagcgtaca
    56701 gctctttggt tccgagtacg ccatgcgtat ctggctggac ccgcaaaaac tcaataaata
    56761 caatctggta ccttccgatg ttatttccca gattaaggtg caaaacaacc agatttccgg
    56821 tggtcaactg ggtggcatgc cacaggcggc agaccagcaa ctaaacgcct cgatcattgt
    56881 gcagacgcgt ctgcaaacgc cggaagaatt tggcaaaatc ctgttgaaag ttcagcaaga
    56941 tggttcgcaa gtgctgctgc gtgatgtcgc ccgcgtcgaa cttggagcgg aagattattc
    57001 caccgtggcg cgctataacg gcaaacctgc tgccgggatc gccatcaaac tggctaccgg
    57061 agcaaacgcc ctggatacct cgcgggcagt caaagaggag ctgaaccgct tatcagccta
    57121 tttccccgca agtctgaaga cggtttatcc ttacgacacc acgccgttta tcgaaatttc
    57181 tattcaggaa gtttttaaaa cactggttga ggctatcatc ctcgtcttcc tggtcatgta
    57241 tctgtttttg cagaatttcc gtgccacaat catcccgacg attgccgtac cggtggttat
    57301 tctcgggacg tttgcgatct tgtcggcggt cggtttcacc atcaacacgt taactatgtt
    57361 cgggatggtg ctggcgatag ggctactggt ggatgacgcc atcgtggtgg tggagaacgt
    57421 cgagcgtgtc attgcggaag ataagctacc accgaaggaa gcgacgcata aatcgatggg
    57481 gcagatccaa cgtgcgctgg tcggtattgc tgttgttctt tccgcagtgt ttatgccgat
    57541 ggcctttatg agcggcgcaa ccggggagat ctaccgccag ttctccatca cgctgatctc
    57601 ctccatgctg ctttcagtat ttgtggcaat gagcctgacc cctgccctgt gcgccaccat
    57661 tctgaaagcc gcgccggaag gcggtcacaa acctaacgcc ctgttcgcac gcttcaacac
    57721 gctgtttgaa aaatcaactc agcactatac cgatagcacc cgctcgctgt tgcgttgtac
    57781 cggtcgctac atggtggtct acctgctgat ttgcgccggg atggcggtgc tgttcctgtg
    57841 tacaccgacc tctttcttac cagaagagga tcagggggta tttatgacca ccgcgcagtt
    57901 accttccggt gccaccatgg ttaacaccac gaaagtgctg caacaggtga cggattatta
    57961 tcttacaaaa gagaaagata atgttcagtc ggtgtttacc gttggcggct ttggcttcag
    58021 cggccagggg caaaacaacg gcctggcgtt tatcagtctc aagccgtggt ctgaacgtgt
    58081 cggtgaggaa aactcggtta ccgcgatcat tcagcgggca atgattgcgt taagcagtat
    58141 caataaagcc gtcgtcttcc cgttcaactt acccgcggtg gctgaactgg gtaccgcgtc
    58201 aggttttgat atggaactgc tggacaacgg taacctgggg cacgaaaaac taacccaggc
    58261 acgaaacgag ctgttatcac tggcagcgca atcgcctgac caggtcaccg gggtacgccc
    58321 gaacggcctg gaagatacgc cgatgttcaa agtgaacgtc aacgccgcga aagctgaggc
    58381 aatgggtgtg gcgctgtctg atatcaacca gacaatttcc accgccttcg gcagcagcta
    58441 cgtgaacgac ttcctcaacc aggggcgggt gaaaaaagtg tatgtccagg caggcacgcc
    58501 gttccgtatg ttgccggata acatcaacca atggtatgta cgcaacgcct ctggcacgat
    58561 ggcaccgctt tctgcctact cgtctaccga atggacctat ggttcaccgc gattggaacg
    58621 ctacaacggc atcccgtcaa tggagatttt aggtgaagcg gcggccggga aaagtaccgg
    58681 tgacgctatg aaatttatgg cagacctggt cgctaaactt ccggcaggcg tcggctactc
    58741 atggaccgga ctgtcgtatc aggaagcgtt atcctcaaac caggctcctg cactgtatgc
    58801 catttcactg gtcgtggtgt tcctcgccct cgccgcactc tatgagagct ggtcaattcc
    58861 gttctcggtg atgttggttg ttccgttagg cgtcgttggc gcattactgg ccaccgatct
    58921 gcgcggctta agtaatgacg tctacttcca ggttggtttg ctgaccacca tcgggctttc
    58981 cgccaagaac gccatcctga ttgtcgaatt tgccgttgag atgatgcaga aagaagggaa
    59041 aacgccgata gaggcaatca tcgaagcggc gcggatgcgt ttacgcccaa tcctgatgac
    59101 ctctctggcc tttattctcg gcgtgctgcc gctggttatc agtcatggtg ccggttctgg
    59161 cgcgcaaaac gcggtaggta ccggcgtgat gggcgggatg tttgccgcaa cagtgctggc
    59221 aatttacttc gttccggtct ttttcgttgt agtggaacat ctctttgccc gctttaaaaa
    59281 agcgtaacgt gtaaatgaga gtaaggttga acgtgaaggt tcagccttac tctttcctgc
    59341 taaccattca ccactcaaca accagctaac cgtcaggaat accaggctta gtacacagca
    59401 ctgaaagtag aaaccactcc agccattcgt catcaatttg aacagcacaa tactgaccca
    59461 cattcccgta atcgtatgaa ttgtcagctt aataatccca aacatgccga gtcttttcct
    59521 cctgaaaata cctataccgc ctggcaaact gcgaaagaga actagccggt agcccggcag
    59581 aaatcatcag ggaagagttt cacatgaagc aggtgtgaga tcctgaccaa tattcaaatg
    59641 caaaatatgt caggaaaagg tacctggcga atgttgcgca aactgatgtg gcgttacacc
    59701 ataatattgt cgaaatgtgt ttataaagta cgacgtactg ctatagccac atttttctgc
    59761 aatagtatgc agaggaattt gacgtaactc gagtaatcgt ctggccatcg acatcctgga
    59821 agcgagtaat attttactga aacaggtatt ttcatcctgc aacttttttt tgatgagact
    59881 ctcgctggta tacattcttt ccgcgatatc gcgcagatac caacgtttgg cgatatcaaa
    59941 gctaataagg cgttcaactt ttcctgaaac agtgctgatg ctattgaaaa gtaaggggat
    60001 cagttctttc ttatgagaaa acatcgataa acaggaaagg tagagcatat tgctcagtat
    60061 cggtgaattt accccggatt cattgaagct atcaaaaatc gccgtcacca gcggcatggg
    60121 gggtgactgg ttaattaata gacggtcttt gctatgtaac cctaaattgc gtggtagctg
    60181 acgaatattt tgtaagtaat ggcaaactgt cagctcatcg atatccagtc gtcgcgtatc
    60241 cggtgaacaa aacgcaaaat cgtctgccaa atttttctcc agcagcacga tactctcgtt
    60301 atgcagcgat attttatgct gttcatgata aatatcgaat gaacgacgaa tgaggatcac
    60361 cgagcagaca tgagtcatga ttatccctta tatttcatac tgcgattatt tcaatttcag
    60421 tatactaatg aaatgttgcc agactgtttc ttagcgaaaa aactgccaga tttggtagga
    60481 ccaaaattta tccacaaagt agttttgcat aacagttaat tgataataag taaatgcagc
    60541 acgaatatat tttcgcactg cgtatagctt atgtttataa aaaaatggct gatcttattt
    60601 ccagtaaaag ttatatttaa cttactgaga gcacaaagtt tcccgtgcca acagggagtg
    60661 ttataacggt ttattagtct ggagacggca gactatcctc ttcccggtcc cctatgccgg
    60721 gtttttttta tgtctgagta aaattctata atcttattcc ttccgcagaa cggtcagtgc
    60781 cgtaaaaatt tccttgcgca acaatacttg ccgccgagtc acggttcggc aatccctgcg
    60841 ccgatcgctc ctgatactct gtgggcgtca tcccataata atttcgaaag acgtaaatga
    60901 aatacgacac gctgtgatat ccacaggaca ctgcgactcg cttaattgaa aagccatgta
    60961 taacaataag ttgcaaagca cgttgcattc tacactcagt aagcaactgt gaatatgatg
    61021 tctcttcttc gcgcaatttt ttctttaaca ggcttggact catcaacagc tcgctggcga
    61081 ttcgggctag tgtccactca tgggcgatat tattattgat aaccgtacaa actcgtgttc
    61141 gcatgttcgg ttgcaaaacg ttcaaaagca gcggtataaa gtgctcatcc tcaagaaaaa
    61201 cagataacaa cgcaaagatt aatgctcttt tacgtagcat ttctgcatag cgaagattct
    61261 tattctgcga tagttgcgca acttcctgaa aaacaggaat atctctgcta cagtgatgaa
    61321 cgagtaatga agaaattttt ttatgtgcat gaagatttaa tgcctcctcc ttgagcatcg
    61381 gcagaaataa tgaaagcgta tcccttgata ctaatacaaa attttcaaca cacttatcta
    61441 ctcgaatttg gcttgcatcc gcaaaaacca ggtcaccgcc attaaaatag cgatattcac
    61501 cattaaccat ggtgagaata tatttatgtc ttgcgtacgc aattagacaa ttcccatgta
    61561 atgattgcat agttgactta atattacata aacatattac tgttaattaa tgtagcacgc
    61621 cgccctctta agtcaaattg attgataaat aaatttaaac gaagcgggag aacaggatga
    61681 taatgtgtga tgttctacgg gcaggatgac tggatttata atacaaaaac gtgtttaaga
    61741 acgcagtgaa attaatagca agcaaataat cccttttcgt gacattaaag gcaatcgcta
    61801 catttaataa acattcatat aacatatatc ttatcaacac gatgaataga cagccaatat
    61861 attattgcga ttaataagca accgaatgcc cagctgtttt tttaaaggct gggcattcgg
    61921 tttttacaac gttatgttat caggt
//
LOCUS       DAHBHC010000019        60664 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHBHC010000019 DAHBHC010000000
VERSION     DAHBHC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.019"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(118..1305)
                     /locus_tag="M2A35_001569"
     CDS             complement(118..1305)
                     /locus_tag="M2A35_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HCB7197885.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMIDFSSEKIHDYIDIIIEDYENIKENAYLVIPQINYTMD
                     LNIEDSSSEELLSDNPLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDLL
                     NAI"
     gene            complement(1327..1866)
                     /locus_tag="M2A35_001570"
     CDS             complement(1327..1866)
                     /locus_tag="M2A35_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197886.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(2123..2467)
                     /gene="tdcR"
                     /locus_tag="M2A35_001571"
     CDS             complement(2123..2467)
                     /gene="tdcR"
                     /locus_tag="M2A35_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCB7197887.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            2656..3594
                     /gene="tdcA"
                     /locus_tag="M2A35_001572"
     CDS             2656..3594
                     /gene="tdcA"
                     /locus_tag="M2A35_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCB7197888.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            3693..4682
                     /gene="tdcB"
                     /locus_tag="M2A35_001573"
     CDS             3693..4682
                     /gene="tdcB"
                     /locus_tag="M2A35_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCB7197889.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            4704..6035
                     /gene="tdcC"
                     /locus_tag="M2A35_001574"
     CDS             4704..6035
                     /gene="tdcC"
                     /locus_tag="M2A35_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCB7197890.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            6061..7269
                     /gene="tdcD"
                     /locus_tag="M2A35_001575"
     CDS             6061..7269
                     /gene="tdcD"
                     /locus_tag="M2A35_001575"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCB7197891.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            7303..9597
                     /gene="tdcE"
                     /locus_tag="M2A35_001576"
     CDS             7303..9597
                     /gene="tdcE"
                     /locus_tag="M2A35_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCB7197892.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYPFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            9611..10000
                     /locus_tag="M2A35_001577"
     CDS             9611..10000
                     /locus_tag="M2A35_001577"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCB7197893.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            10072..11436
                     /gene="tdcG"
                     /locus_tag="M2A35_001578"
     CDS             10072..11436
                     /gene="tdcG"
                     /locus_tag="M2A35_001578"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCB7197894.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            11711..13042
                     /locus_tag="M2A35_001579"
     CDS             11711..13042
                     /locus_tag="M2A35_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCB7197895.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            13070..14380
                     /locus_tag="M2A35_001580"
     CDS             13070..14380
                     /locus_tag="M2A35_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCB7197896.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14592..14756)
                     /locus_tag="M2A35_001581"
     CDS             complement(14592..14756)
                     /locus_tag="M2A35_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197897.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(14779..15480)
                     /locus_tag="M2A35_001582"
     CDS             complement(14779..15480)
                     /locus_tag="M2A35_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCB7197898.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            15585..16481
                     /gene="yhaJ"
                     /locus_tag="M2A35_001583"
     CDS             15585..16481
                     /gene="yhaJ"
                     /locus_tag="M2A35_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCB7197899.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(16532..16888)
                     /locus_tag="M2A35_001584"
     CDS             complement(16532..16888)
                     /locus_tag="M2A35_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCB7197900.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(17130..17447)
                     /locus_tag="M2A35_001585"
     CDS             complement(17130..17447)
                     /locus_tag="M2A35_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCB7197901.1"
                     /translation="MDWYLKVLKNYAGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWALLFLIPFIGWLIIIVFNCQAGTPGENRFGPDPK
                     LEP"
     gene            complement(17740..18726)
                     /gene="yqjG"
                     /locus_tag="M2A35_001586"
     CDS             complement(17740..18726)
                     /gene="yqjG"
                     /locus_tag="M2A35_001586"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCB7197902.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(18796..19188)
                     /locus_tag="M2A35_001587"
     CDS             complement(18796..19188)
                     /locus_tag="M2A35_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCB7197903.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(19374..19673)
                     /locus_tag="M2A35_001588"
     CDS             complement(19374..19673)
                     /locus_tag="M2A35_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCB7197904.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(19663..20067)
                     /locus_tag="M2A35_001589"
     CDS             complement(19663..20067)
                     /locus_tag="M2A35_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCB7197905.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(20070..20375)
                     /locus_tag="M2A35_001590"
     CDS             complement(20070..20375)
                     /locus_tag="M2A35_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCB7197906.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(20413..20781)
                     /locus_tag="M2A35_001591"
     CDS             complement(20413..20781)
                     /locus_tag="M2A35_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCB7197907.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(20928..21311)
                     /gene="mzrA"
                     /locus_tag="M2A35_001592"
     CDS             complement(20928..21311)
                     /gene="mzrA"
                     /locus_tag="M2A35_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCB7197908.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(21315..21977)
                     /locus_tag="M2A35_001593"
     CDS             complement(21315..21977)
                     /locus_tag="M2A35_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7197909.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(22322..23098)
                     /gene="exuR"
                     /locus_tag="M2A35_001594"
     CDS             complement(22322..23098)
                     /gene="exuR"
                     /locus_tag="M2A35_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCB7197910.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            23394..24110
                     /locus_tag="M2A35_001595"
     CDS             23394..24110
                     /locus_tag="M2A35_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCB7197911.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINNQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            24140..24640
                     /locus_tag="M2A35_001596"
     CDS             24140..24640
                     /locus_tag="M2A35_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7197912.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            24732..27416
                     /locus_tag="M2A35_001597"
     CDS             24732..27416
                     /locus_tag="M2A35_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCB7197913.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            27413..28501
                     /locus_tag="M2A35_001598"
     CDS             27413..28501
                     /locus_tag="M2A35_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCB7197914.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(28589..29887)
                     /gene="exuT"
                     /locus_tag="M2A35_001599"
     CDS             complement(28589..29887)
                     /gene="exuT"
                     /locus_tag="M2A35_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCB7197915.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVCALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            30370..31782
                     /gene="uxaC"
                     /locus_tag="M2A35_001600"
     CDS             30370..31782
                     /gene="uxaC"
                     /locus_tag="M2A35_001600"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCB7197916.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            31797..33284
                     /gene="uxaA"
                     /locus_tag="M2A35_001601"
     CDS             31797..33284
                     /gene="uxaA"
                     /locus_tag="M2A35_001601"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCB7197917.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            33367..33918
                     /locus_tag="M2A35_001602"
     CDS             33367..33918
                     /locus_tag="M2A35_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCB7197918.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(33923..35167)
                     /gene="sstT"
                     /locus_tag="M2A35_001603"
     CDS             complement(33923..35167)
                     /gene="sstT"
                     /locus_tag="M2A35_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCB7197919.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(35566..36531)
                     /gene="alx"
                     /locus_tag="M2A35_001604"
     CDS             complement(35566..36531)
                     /gene="alx"
                     /locus_tag="M2A35_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCB7197920.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(36814..37800)
                     /locus_tag="M2A35_001605"
     CDS             complement(36814..37800)
                     /locus_tag="M2A35_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCB7197921.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VGAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(37879..38562)
                     /locus_tag="M2A35_001606"
     CDS             complement(37879..38562)
                     /locus_tag="M2A35_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCB7197922.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(38639..39142)
                     /locus_tag="M2A35_001607"
     CDS             complement(38639..39142)
                     /locus_tag="M2A35_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCB7197923.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            39227..40363
                     /gene="rlmG"
                     /locus_tag="M2A35_001608"
     CDS             39227..40363
                     /gene="rlmG"
                     /locus_tag="M2A35_001608"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCB7197924.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            40554..40637
                     /locus_tag="M2A35_001609"
                     /pseudo
     CDS             40554..40637
                     /locus_tag="M2A35_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            40634..41050
                     /gene="higA"
                     /locus_tag="M2A35_001610"
     CDS             40634..41050
                     /gene="higA"
                     /locus_tag="M2A35_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCB7197925.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41095..43113)
                     /gene="fadH"
                     /locus_tag="M2A35_001611"
     CDS             complement(41095..43113)
                     /gene="fadH"
                     /locus_tag="M2A35_001611"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCB7197926.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(43439..45790)
                     /gene="ygjK"
                     /locus_tag="M2A35_001612"
     CDS             complement(43439..45790)
                     /gene="ygjK"
                     /locus_tag="M2A35_001612"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCB7197927.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(45807..46877)
                     /gene="ygjJ"
                     /locus_tag="M2A35_001613"
     CDS             complement(45807..46877)
                     /gene="ygjJ"
                     /locus_tag="M2A35_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCB7197928.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(47011..48444)
                     /locus_tag="M2A35_001614"
     CDS             complement(47011..48444)
                     /locus_tag="M2A35_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCB7197929.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(48507..48956)
                     /locus_tag="M2A35_001615"
     CDS             complement(48507..48956)
                     /locus_tag="M2A35_001615"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCB7197930.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(48953..52045)
                     /gene="ebgA"
                     /locus_tag="M2A35_001616"
     CDS             complement(48953..52045)
                     /gene="ebgA"
                     /locus_tag="M2A35_001616"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCB7197931.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(52229..53212)
                     /gene="ebgR"
                     /locus_tag="M2A35_001617"
     CDS             complement(52229..53212)
                     /gene="ebgR"
                     /locus_tag="M2A35_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCB7197932.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            53431..53763
                     /locus_tag="M2A35_001618"
     CDS             53431..53763
                     /locus_tag="M2A35_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCB7197933.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(53805..55184)
                     /gene="ygjG"
                     /locus_tag="M2A35_001619"
     CDS             complement(53805..55184)
                     /gene="ygjG"
                     /locus_tag="M2A35_001619"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCB7197934.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            55602..57122
                     /gene="aer"
                     /locus_tag="M2A35_001620"
     CDS             55602..57122
                     /gene="aer"
                     /locus_tag="M2A35_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCB7197935.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(57276..57899)
                     /gene="nfeR"
                     /locus_tag="M2A35_001621"
                     /gene_synonym="yqjI"
     CDS             complement(57276..57899)
                     /gene="nfeR"
                     /locus_tag="M2A35_001621"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCB7197936.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            58176..58940
                     /gene="nfeF"
                     /locus_tag="M2A35_001622"
     CDS             58176..58940
                     /gene="nfeF"
                     /locus_tag="M2A35_001622"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCB7197937.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            59220..60650
                     /locus_tag="M2A35_001623"
     CDS             59220..60650
                     /locus_tag="M2A35_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCB7197938.1"
                     /translation="MKLFRFSKVKIAELPTPEHGQVEYGDTEVNGLRVRVGSSGVKSF
                     CISRKRNGKFIRATLGRFPDLSVDNARAKAMELLGEVATTGINPNIAKRTNNKAMITL
                     SEAIDAYVKNRGHRLKPSTEKQYRSILQNYSGDWMAHSLASISRERVEQRHKAITDGA
                     VWFGADKTTLRAGVGTGSKAQADLWARALRAVCRFAHDHYRDDAGNTLLPDPPTLVLS
                     TKRQWHGTVRKTERIRLHDFPRWFAAVAAVAAVRDQGEQERDDIAVTVCDAVEMALFT
                     GLRKSEVFGLTWDRVNMGGRFFWIDTTKNGDPLELPITDTLRKMFLRRLKNKAADDVL
                     VFPGVKGVIQETRHIIDRISAATIPEANDEMLFPVPFKWHDARRTFGTIAELVGVGNY
                     ILKRLMNHRTMRSADVTQGYLHFGADELLEPASRIERAILEHAGLVESKKSIDAQLLS
                     ALEGMSDDEKRRLLFAIVQEKNDIYG"
BASE COUNT        15118 a        14770 c        15621 g        15155 t
ORIGIN      
        1 ttttgcggtt gccatcagtt tcaaacccgc gtcagtgagt ttttgctttt ggtgcacagg
       61 gtaagatgaa taaatatcta cggtattaat aatagacttt gtaagaagtt attaagtcta
      121 tattgcattt aaaaggtcgt aattatacgt atagtcctct ctggtaaaat attgattctc
      181 tgagttatat gcttcatatg tgttatattc cccgacctca aaaccattgt ccggagaatt
      241 ttcgtctttc tcgagggggt tatctgaaag taactcttct gagctagagt cttctatgtt
      301 taaatccata gtataattaa tttggggaat taccagataa gcattttctt ttatgttttc
      361 ataatcttca atgattatgt cgatataatc atgtattttt tctgatgaga aatctatcat
      421 ctctggtaat ttaattctgt tcaagcaatt attgattttc gatgagcttt tatatatttc
      481 ttttgttaat tcattttcta atttatctgt aagagttgct cttggtatag attttataat
      541 tgcaaatctc atgattttta attgttcttc ggtaatatct ccaagctcat ttaaacaact
      601 ttcgacaagc ttaatttttt cttcagaata catatcgcta atgcttgtta gaacaggcgt
      661 tatttttagt aaataatccc aggagattgt ggtgttttgt tttgaactaa cgaaatttac
      721 ctgaaacatg ttggcattag taaaatctgt gtcatccaat ttaaaaggat ttaaaattgc
      781 attggccata tgtttatctg taaaatcata aagttctaaa tcgatattcc gtaagtccag
      841 agaggataga tcaactatac ctccagtccc acaactaaaa actaagttca acctcctgat
      901 ttcattgtaa atgtctttca atgggaaatt aacattgata ttgcctgtgg atatttcctg
      961 gttttttaac ggttcattaa ttgtaagttc acaggtattg tctgatcgtt gaataattag
     1021 ggcgttataa ccaaaaaaat ccatacgaag agacgaatca ttataaataa ttacattttg
     1081 taagtacgct aattcttttt gaaaatcacc agtgataatg cattttccac ctggttgtaa
     1141 cgcgttaagt aaagtgacca tttgaataga ggtctgtttt agtttttcta tacctggtgc
     1201 tagtgcttcg aggttattaa ctatggggct ttcaggaaga tcattcattt ttcgacggac
     1261 taaatcacta ctaatatcat taccaattga tgaaacaggg aacattattt tatcctttat
     1321 aaataatcaa taaaaatcat tattaaaatg atcgggtgaa aaaaagagaa tttgccaaca
     1381 atctgaagta atgctggttt tattaaagtg cgcaggaggt gttaagatgg cttcttttcg
     1441 ggtgaatttt gcacaatact tagaaatcgt ctccttaata cattccagct cttttttatt
     1501 gacggctgag caagtacatt gttcaccaaa aggtgcaaaa gtgcctacat ctaatgtttg
     1561 tatgatataa tcgtcatgtt ctcctgtaga aatatctatg atgaatggac tcatattttc
     1621 cggttggatt cggatcatga cagaaggaac atcgccttga tagaaattca atgcagtaat
     1681 tgcagataat actctccgca gttcattact tatactgtaa attttattaa cgtcattggt
     1741 ggagacaatt tctgcaaact ttctttttac cgtccaataa tcaatatttc tattgtgatt
     1801 gttaaccact gcgtgcattg gcgggattga agggtgaaaa atatgtgcaa ttgggaagcc
     1861 tttcattgat gcctgccttg tcgtcatgac aaacaataat agatgcttat tttcctcgca
     1921 gggttctttc gcactatcaa aaaacgctca tttacccggc gttacagtaa acgaagcaac
     1981 attctgtggc ctacgacaga tcaaaagctc tgtgaaaatt tcccttgttg tctttgtctg
     2041 accttatcgc ggcgatcagc aattattcgt tttgtgatcc tcaacacaaa atgctattcg
     2101 tcgcctatga ggggagaaga atttatggaa aacgattgca ttcacaaaat gacgtcctgt
     2161 cagaagagaa aaagttgacg ttaatcaatt gttcgagatg atcaatacct gtattcgaaa
     2221 ttttaaaata ttgataaaat aaatgtttgt tctccaacaa atagcgtgaa aaaatgtgac
     2281 gtgccgcagt atttatcgct gaaaatggtt tatttataat aaaatttgag aacttgctca
     2341 ttaaatttaa ctcaaatttt gcctggtaat tatccggtaa ttgcttgaaa tatggtctca
     2401 gccccttttt tgtattaacc acataacgaa tgatgttatc gccataaaat atggttatcc
     2461 ccgtcatttt ttttgacaaa aatcagggtt tatgctgatt tttatgcttt aacttgttga
     2521 tatttaaagg tgtttaattg taataacgat accctggaaa gtattgaaag ttaatttgtg
     2581 agtggtcgca catatcctgt tcatttcatt ttgatacact tcatgccgtc aatgaggtaa
     2641 ttaacgtagg tcgttatgag cactattctt cttccgaaaa cgcagcacct ggtagtcttt
     2701 caggaagtca ttagaagtgg ctctatcggc tcggctgcaa aagaattagg gttaactcaa
     2761 ccagccgtca gtaaaatcat taacgatatt gaagattatt ttggtgtgga attagtggtg
     2821 cggaaaaata ccggtgtaac attaacacct gccggtcaat tgttactctc ccgttccgaa
     2881 tccattaccc gtgaaatgaa aaatatggtt aatgagataa gtggtatgtc ttctgaggcg
     2941 gtggtggaag tctcatttgg ttttccttca ttgattggtt ttacttttat gtcagggatg
     3001 atcaacaagt tcaaagaggt gttcccgaaa gcgcaggttt ctatgtatga agcgcaactg
     3061 tcttcgttct taccggcaat ccgcgacggt cgactggatt ttgcgattgg tacgttaagt
     3121 gcagaaatga agcttcagga tttacatgtt gagccgctgt tcgagtccga gtttgtgttg
     3181 gtagccagta agtcccgaac atgcaccggc accaccacgc tggagtcgtt gaagaacgaa
     3241 cagtgggtgt tgccacaaac caatatgggg tactacagcg agcttcttac tacgttacaa
     3301 agaaatggca tcagtattga aaacatcgtt aaaaccgact cagtcgtgac aatttataat
     3361 cttgttctca atgctgattt cttaactgta attccttgtg atatgacgtc accttttggt
     3421 tcaaatcaat ttattactat tccggttgaa gaaacattac ctgtggcaca atatgccgcg
     3481 gtatggtcga aaaattatcg tattaaaaaa gcagcatcgg ttttggtgga attagccaaa
     3541 gagtattcat cttataatgg gtgtagacga aggcaattaa tagaagttgg ttagttattt
     3601 gtttttattt aaacataaat aatccacctg tctgttttgc aggtgtcggt tacggttacc
     3661 tacatattta attcaggcga agaggtttta taatgcatat tacatacgat ctgccggttg
     3721 ctattgatga cattattgaa gcgaaacaac gactggctgg gcgaatttat aaaacaggca
     3781 tgcctcgctc aaactatttt agtgaacgtt gcaaaggtga aatattcctg aagtttgaaa
     3841 atatgcagcg tacgggttca tttaaaattc gtggcgcatt taataaatta agttcactga
     3901 ttgatgcgga aaaacgtaaa ggtgtagtgg cctgttctgc gggcaaccat gcgcaagggg
     3961 tttcgctctc ctgcgcgatg ctgggtatcg acggtaaagt ggtgatgcca aaaggtgcgc
     4021 caaaatccaa agtagcggca acgtgcgact actccgcaga agtcgttctg catggtgata
     4081 atttcaacga cactatcgct aaagtgagcg aaattgtcga aatggaaggt cgtattttta
     4141 tcccacctta cgatgatccg aaagtgattg ctggccaggg aacgattggt ctggaaatta
     4201 tggaagatct ctatgatgtc gataacgtga ttgtgccaat tggtggtggt ggtttaattg
     4261 ctggtattgc ggtggcaatt aaatctatta acccgaccat tcgtgttatt ggcgtacagt
     4321 cagaaaacgt tcacggcatg gcggcttctt tccactccgg agaaataacc acgcaccgaa
     4381 ctaccggcac cctggcggat ggttgtgatg tctcccgccc gggtaattta acttacgaaa
     4441 tcgttcgtga attagtcgat gacatcgtgc tggtcagcga agacgaaatc agaaacagta
     4501 tgattgcctt aattcagcgc aataaagtcg tcaccgaagg cgcaggcgct ctggcatgtg
     4561 ctgcattatt aagcggtaaa ttagaccaat atattcaaaa cagaaaaacc gtcagtatta
     4621 tttccggcgg caatatcgat ctttctcgcg tctctcaaat caccggtttc gttgacgctt
     4681 aattaattcg ttgaggatag gatatgagta cttcagatag cattgtatcc agccagacaa
     4741 aacaatcgtc ctggcgtaaa tcagatacca catggacgtt aggcttgttt ggtacggcaa
     4801 tcggcgccgg ggtgctgttc ttccctatcc gcgcaggttt tggcggactg atcccgattc
     4861 ttctgatgtt ggtattggca taccccatcg cattttattg ccaccgggcg ctggcgcgtc
     4921 tgtgtctttc tggctctaac ccttccggca acattacgga aacggtggaa gagcattttg
     4981 gtaaaactgg cggcgtggtt atcacgttcc tgtacttctt cgcgatttgc ccactgctgt
     5041 ggatttatgg cgttactatt accaatacct ttatgacgtt ctgggaaaac cagctcggat
     5101 ttgcaccgct gaatcgcggc tttgtggcgc tgttcctgtt gctgctgatg gctttcgtca
     5161 tctggtttgg taaggatctg atggttaaag tgatgagcta cctggtatgg ccgtttatcg
     5221 ccagcctggt gctgatttct ctgtcgctga tcccttactg gaactctgca gttatcgacc
     5281 aggttgacct cggttcgctg tcgttaaccg gtcatgacgg tatcctgatc actgtctggc
     5341 tggggatttc catcatggtt ttctccttta acttctcgcc aatcgtctct tccttcgtgg
     5401 tttctaaacg tgaagagtat gagaaagact tcggtcgcga cttcaccgaa cgtaaatgtt
     5461 cccaaatcat ttctcgtgcc agcatgctga tggttgcagt ggtgatgttc tttgccttta
     5521 gctgcctgtt tacgctgtct ccggccaaca tggcggaagc caaagcgcag aatattccag
     5581 tgctttctta tctggctaac cactttgcgt ccatgaccgg taccaaaaca acgttcgcga
     5641 ttacactgga atatgcggct tccatcatcg cactcgtggc tatcttcaaa tctttcttcg
     5701 gtcactatct ggggacgctg gaaggcttga atggtctgat tctgaagttc ggttataaag
     5761 gcgacaaaac taaagtgtcg ctgggtaaac tgaacactat cagcatgatc ttcatcatgg
     5821 gctccacctg ggttgttgcc tacgccaacc cgaacatcct cgaccttatt gaagccatgg
     5881 gcgcaccgat tatcgcatcc ctgctgtgcc tgttgccgat gtatgccatc cgtaaagcgc
     5941 cgtctctggc gaaataccgt ggtcgtctgg ataacgtgtt tgttaccgtg attggtctgc
     6001 tgaccatcct gaacatcgta tacaaactgt tttaatccgt aactcaggat gagaaaagag
     6061 atgaatgaat ttccggttgt tttggttatt aactgtggtt cgtcttcgat taagttttcc
     6121 gtactcgatg ccagcgactg tgaagtatta atgtcaggta ttgccgacgg tattaactcg
     6181 gaaaatgcat tcttatccgt aaatggggga gagccagcac cgctggctca ccacagctac
     6241 gaaggtgcat tgaaggcaat tgcatttgaa ctggaaaaac ggaatttaaa tgacagtgtg
     6301 gccttaattg gccaccgcat cgctcacggc ggcagtattt ttaccgagtc cgccattatt
     6361 accgatgaag tcattgataa tatccgtcgc gtttctccac tggcacccct gcataattac
     6421 gccaatttaa gtggtattga atcggcgcag caattatttc cgggcgtaac tcaggtggcg
     6481 gtatttgata ccagtttcca ccagacgatg gctccggaag cttatttata cggcctgccg
     6541 tggaaatatt atgaagagtt aggtgtacgc cgttatggtt tccacggcac gtcgcaccgc
     6601 tatgtttccc agcgcgcaca ttcgctgctg aatctggcgg aagatgactc cggcctggtt
     6661 gtggcgcatc ttggcaatgg cgcgtcaatc tgcgcggttc gcaacggtca gagtgttgat
     6721 acttcaatgg gaatgacgcc gctggaaggc ttgatgatgg gtacccgcag tggcgatgtc
     6781 gactttggtg cgatgtcctg ggtcgccagc caaaccaacc agagcctggg tgacctggaa
     6841 cgcgtagtga ataaagagtc gggattatta ggtatttccg gtctttcttc ggatttacgt
     6901 gttctggaaa aagcctggca tgaaggtcac gaacgcgcgc aactggcaat taaaaccttt
     6961 gttcaccgaa ttgcccgtca tattgccgga cacgcagctt cattacgtcg cctggatgga
     7021 attatattca ccggcggaat aggagagaat tcaagcttaa ttcgtcgtct ggtcatggaa
     7081 catttggctg tattaggcgt agagattgat acagaaatga ataatcgctc taactcctgt
     7141 ggtgagcgaa ttgtttccag tgaaaatgcg cgtgtcattt gtgccgttat tccgactaac
     7201 gaagaaaaaa tgattgcttt ggatgccatt catttaggca aagttaacgc gcccgcagaa
     7261 tttgcataat ttagttgaag tattgtagag agattatttt tcatgaaggt agatattgat
     7321 accagcgata agctgtacgc cgacgcatgg cttggcttta aaggtacgga ctggaaaaac
     7381 gaaattaatg tccgcgattt tattcaacat aactatacac cgtatgaagg cgatgaatct
     7441 ttcctcgccg aagcgacgcc tgccaccacg gaattgtggg aaaaagtaat ggaaggcatc
     7501 cgtatcgaaa atgcaaccca cgcgccggtt gatttcgata ccaatattgc caccacaatt
     7561 accgctcatg atgcgggata tattaaccag ccgctggaaa aaattgttgg cctgcaaacg
     7621 gatgcgccgt tgaaacgtgc gctacacccg ttcggtggca ttaatatgat taaaagttca
     7681 ttccacgcct atggccgtga aatggacagt gaatttgaat atccgtttac cgatctgcgt
     7741 aaaacccata accagggcgt atttgatgtt tactcaccgg atatgctgcg ctgccgtaaa
     7801 tctggcgtgc tgaccggttt accagatggc tatggccgtg ggcgcattat cggtgactat
     7861 cgccgcgtag cgctgtatgg catcagttat ctggtacgtg aacgcgaact gcaatttgcc
     7921 gatctccagt ctcgtctgga aaaaggcgag gatctggaag ccaccatccg tctgcgtgag
     7981 gagctggcag agcatcgtca tgcgctgttg cagattcagg aaatggcggc gaaatatggc
     8041 tttgatatct ctcgcccggc gcagaatgcg caggaagcgg tgcagtggct ctacttcgct
     8101 tatctggcgg cagtgaaatc gcaaaatggc ggcgcgatgt cgctgggccg cacggcatcg
     8161 ttcctcgata tctacattga gcgcgacttt aaagctggcg tactcaatga gcagcaggca
     8221 caggaactga tcgatcactt catcatgaag atccgtatgg tacgcttcct gcgtacaccg
     8281 gaatttgatt cgctgttctc cggcgaccca atctgggcga cggaagtgat cggcgggatg
     8341 gggctggacg gtcgtacgct ggtgaccaaa aactccttcc gctatttgca caccctgcac
     8401 actatggggc cggcaccgga acctaacctg accattcttt ggtcggaaga attaccgatt
     8461 gccttcaaaa aatatgccgc gcaggtgtcg atcgtcacct cttccttgca gtatgaaaat
     8521 gacgatctga tgcgtactga cttcaacagc gacgattacg cgattgcctg ctgcgtcagc
     8581 ccaatggtga ttggtaagca aatgcagttc tttggtgcac gcgctaacct ggcgaaaacg
     8641 ctgctctacg caattaacgg cggggtggac gagaagctga agattcaggt cgggccgaaa
     8701 acagcaccgc tgatggacga cgtgctggat tacgacaaag tgatggacag cctcgatcac
     8761 ttcatggact ggctggcggt gcagtacatc agcgcgctga atatcattca ctacatgcac
     8821 gacaagtaca gctacgaagc ttcgctgatg gcgctgcacg atcgtgatgt ctatcgcact
     8881 atggcatgcg gcatcgcggg cctgtcggtg gcgacggact ccctgtctgc catcaaatat
     8941 gcccgcgtga aaccaatccg tgacgaaaac ggcctggcgg tggactttga aatcgacggt
     9001 gaatatccgc agtacggcaa caacgacgag cgcgtagaca gcattgcctg cgacctggtt
     9061 gaacgcttta tgaagaaaat taaagcgctg ccaacctatc gcaacgccgt ccctacccag
     9121 tcgattctga ctatcacttc taacgtggtg tacggccaga aaaccggtaa tacgccggac
     9181 ggtcgtcgcg ccggaacacc gttcgcgccg ggcgctaacc cgatgcatgg tcgtgaccgc
     9241 aaaggtgccg tggcctcatt gacgtcggtg gcgaaactgc cgttcaccta cgccaaagat
     9301 gggatctcgt acaccttctc aatcgttcct gcggcgctgg gcaaagaaga tccagtacgt
     9361 aaaaccaacc ttgtcggcct gctggatggg tatttccacc acgaagcgga tgtcgaaggc
     9421 ggtcaacacc tcaacgtcaa cgtaatgaat cgggaaatgc tgctggatgc catcgagcac
     9481 ccggaaaaat atcctaacct gacaatccgt gtctctggct acgccgtgcg cttcaacgca
     9541 ctgacccgtg aacagcaaca ggatgttatt tcacgtactt ttacccaggc gctctgacgc
     9601 cggaggatgt atgaaaaaga ttatcgaaac gcaacgtgcc ccaggcgcaa tcggccctta
     9661 tgttcagggc gttgatttag gcagcatggt cttcacctcc gggcaaattc cggtttgccc
     9721 gcagaccggt gagatcccgg ctgatgtgca agatcaggcg cgtttaagcc tcgaaaacgt
     9781 caaagcgatc gtggttgctg ccgggctgag cgtgggcgat atcatcaaga tgaccgtgtt
     9841 catcaccgat ctgaatgatt ttgccaccat caatgaagtc tataagcagt tcttcgatga
     9901 gcatcaggcg acctacccga cccggagctg tgtgcaggtt gcgcgtttgc cgaaagatgt
     9961 gaagctggaa attgaagcta tcgcagtacg tagtgcgtaa agcctcgtga gcgggacggt
    10021 cgcaaggtcg ttccgctcca cttcactgaa cggcaatccg agggtgtgga tatgattagt
    10081 gcattcgata ttttcaaaat tgggattggt ccctccagtt cgcataccgt ggggccaatg
    10141 aatgccggaa aaagttttat tgatcggctg gaaagtagcg gcttattaac cgcgacgagc
    10201 catattgtgg tcgatctgta tgggtcgttg tcactgacgg gcaaaggcca tgccacggat
    10261 gtcgccatta tcatgggact ggcaggaaac agtccgcagg atgttgtcat tgatgagatc
    10321 cctgcattta tagagttagt aacgcgcagc gggcggctgc cagtggcatc tggtgcgcat
    10381 attgttgatt ttcctgtagc aaagaacatt atcttccatc ccgaaatgtt gcctcgccat
    10441 gagaacggaa tgcggatcac tgcctggaag ggacaggaag cgctattaag taaaacctat
    10501 tactctgtcg gcggcgggtt tattgtcgaa gaagaacact tcggcctgtc gcacgatgtc
    10561 gaaacgtccg taccttacga tttccactca gcaggtgaac tgctgaaaat gtgtgattac
    10621 aacggcctgt ctatatctgg cctgatgatg cacaacgagc tggcgctgcg cagcaaagcg
    10681 gaaattgacg cagggtttgc ccgtatctgg caagtgatgc atgacggtat tgaacgcggg
    10741 atgaacactg aaggcgtgct gcctggtccg ctcaatgtgc cgcgtcgtgc cgtagcgctg
    10801 cgtcgtcagc tggtttccag cgataacatc tctaacgatc cgatgaatgt catcgactgg
    10861 atcaacatgt acgcgctggc ggttagtgaa gaaaacgcag ctggcgggcg cgtggtaacg
    10921 gcaccgacta acggtgcgtg cggcattatt ccggcagtgc tggcttatta cgataagttc
    10981 cgtcgtccgg taaacgagcg gtcaattgcc cgctattttc tggcagcggg ggctattggc
    11041 gcgctgtata aaatgaacgc ctccatctct ggcgcggagg tcggctgtca gggggagatt
    11101 ggcgtggcct gttcaatggc ggcggcaggg ttaactgaac tactgggcgg cagtccggcg
    11161 caggtatgca atgcggcgga aatcgcgatg gagcataacc ttgggctgac ctgcgatccg
    11221 gttgccggac aggtacaaat cccgtgcatt gaacgtaatg ccattaatgc cgtgaaagca
    11281 gtaaacgccg cgcggatggc gatgcgccgc acctcggcac cgcgtgtttc actcgataaa
    11341 gtgatcgaga cgatgtatga aaccggcaaa gatatgaacg ataaataccg cgaaacatca
    11401 cgcggaggac tggccattaa agtggtctgc ggctgacatt gctgtcagct ttcatccttg
    11461 ggtgcacaaa tgtgcacctt ttttttgtga tctgccgcaa atagagaatg aatattctct
    11521 tgcgattaat ttagtagaaa attcttatcc atctccgtta tttcaaagtc ttgattgtga
    11581 agattttctc tgacctcgcg cgtatcattt ctttattcta ataactcgcc tgcaaaaacg
    11641 tagaccgcat atcgccttga tgcggctcgg aaaaaaataa taagccctac aaaatattga
    11701 gcgagaaatt atggaaattg catcgaataa aggcgtcatt gcagacgctt cgaccccggc
    11761 gggtcgtgct ggaatgagtg agagcgagtg gcgagaagcg atcaaattcg acagtactga
    11821 caccggctgg gtgattatga gtatcgggat ggcgattggc gcggggattg tttttctccc
    11881 ggtgcaggtc ggtttgatgg gattgtgggt atttttgctc tcatcggtga ttggttaccc
    11941 ggcaatgtat ctgtttcagc ggttgtttat taatacgctg gcagaatcac cagaatgtaa
    12001 agattacccg agtgtcatta gcggttattt aggtaaaaac tggggcatcc tgttaggtgc
    12061 gctctatttc gtaatgctgg tgatttggat gttcgtctat tccaccgcca tcaccaacga
    12121 tagtgcttcc tatctgcata ccttcggcgt gacggaaggg ttgctgtcag acagtccctt
    12181 ttatggtctg gtactgattt gcattctggt ggcgatctct tcacgcggcg agaaattgtt
    12241 attcaaaatt tcgaccggca tggtgctgac caagctgctg gtggtcgcgg cgctgggcgt
    12301 gtcgatggtg ggaatgtggc atctgtataa cgtcggttcg ctaccgccgc tggggctgct
    12361 ggtgaaaaac gccattatta cgctgccgtt taccctgacg tcgattctgt ttatccagac
    12421 gttaagcccg atggtgatct cttatcgctc gcgggaaaaa tcaattgaag tggcgcggca
    12481 taaagcattg cgggcaatga atatcgcgtt tggcattttg tttgtcaccg tctttttcta
    12541 cgccgtgtcg ttcacgctgg cgatgggaca tgacgaagcg gtaaaagcct atgagcagaa
    12601 tatttccgcg ctggcgattg ccgcacagtt tattagcggt gacggcgcag cgtgggtgaa
    12661 agtggtcagc gtcattctca atatctttgc tgtcatgact gcattctttg gcgtctattt
    12721 aggctttcgc gaagcaacgc aagggatcgt aatgaacatc ctgcgtcgca agatgcctgc
    12781 cgagaagatt aacgaaaatc ttgttcagcg cggcatcatg attttcgcca ttttgctggc
    12841 ctggagcgcc atcgtactga acgcaccggt gttgagcttc acctctatct gtagcccgat
    12901 tttcggcatg gtagggtgcc tgatcccggc gtggctggtt tacaaagtac cggcattgca
    12961 caaatacaaa gggatgtctc tgtacctgat tatcgtcact ggtttgttgc tttgtgtttc
    13021 tccgttcctg gcattttctt gatttacctg aaattttaag gtttttaata tgtttgattc
    13081 gactttaaat ccgttatggc agcgttacat cctcgccgtt caggaggaag taaaaccggc
    13141 gctgggatgt actgaaccga tttcactggc gctggcggcg gcggttgctg cggcagaact
    13201 ggaaggtccg gttgaacgtg tagaagcctg ggtttcgcca aatctgatga agaacggtct
    13261 gggcgtcacc gttcccggca cgggaatggt ggggctgccg attgcggcgg cgctgggggc
    13321 gttaggtgga aatgccaacg ccgggctgga agtgctgaaa gacgcaactg cgcaggcaat
    13381 tgccgatgcc aaagcactgc tggcggcggg gaaagtctcc gttaagatcc aggaaccttg
    13441 caatgaaatc ctcttctcac gcgccaaagt ctggaacggt gagaagtggg cgtgtgtcac
    13501 catcgtcggc gggcatacca acattgtgca tattgagacg cacaatggtg tggtgtttac
    13561 ccagcaggcg tgtgtggcag agggcgagca agagtctccg ctgacggtgc tttccagaac
    13621 gacgctggct gagatcctga agttcgtcaa tgaagtcccg tttgcggcga tccgctttat
    13681 tctcgattcc gcgaagctaa attgtgcgtt atcgcaggaa ggtttgagcg gtaagtgggg
    13741 gctgcatatt ggcgcgacgc tggaaaaaca gtgcgagcgc ggtttgctgg cgaaagatct
    13801 ctcttcatcc attgtgattc gtaccagcgc ggcatccgat gcgcgtatgg gcggcgctac
    13861 gcttccggct atgagtaact ccggctcggg taaccagggg atcaccgcaa caatgcctgt
    13921 ggtggtggta gcagaacact tcggagcgga tgatgaacgg ctggcgcgtg cgctgatgct
    13981 ttctcatttg agcgcaattt acatccataa ccagttaccg cgtttgtctg cactttgtgc
    14041 cgcaacgacc gcagcaatgg gggccgccgc cgggatggca tggctggtgg atgggcgtta
    14101 tgaaaccatc tcgatggcga tcagcagtat gatcggcgat gtcagcggca tgatttgcga
    14161 tggtgcgtcg aacagctgcg cgatgaaggt ttcgaccagt gcttcggctg cgtggaaagc
    14221 ggtgttaatg gcgctggatg ataccgccgt gaccggcaat gaagggatcg tggcgcatga
    14281 tgttgagcag tcgattgcca acctgtgtgc gttagcaagc cattcgatgc agcaaacgga
    14341 tcggcagatt atcgagatta tggcgagcaa ggccagataa acgccaggtt ttgagtattg
    14401 tgccggatgc gatgctggcg caccttatcc ggcctacggg gtgctcaact tgttgatgtt
    14461 gttgtgtttt gtacctgatg ccggatgcga tgctggcgca tcttatccgg cctacgggtt
    14521 gtttaattcg ttaatgttgt tggattttgt aggccggata aggcgttttc gccgcatccg
    14581 gcaacctgtc tttacgcggt agcttcatcc tcggctaaac gcgtttcagc atccgcgacg
    14641 atggcttcca gctcgctcaa catctcttca tagccaaaat ttttggcggt gcgagcaggt
    14701 tctttcgcca ccacgggctt cactggctgg cgctttttgg ccgatttttt actcatcatc
    14761 atctccctgt aaaaacaact agacaggcaa atctatcagc aaagcgcgca gtggggaatc
    14821 ggcaaccagc gtaatgttag cctcgtcacg aataaacgcc ccatcaccgc aggtcagcgc
    14881 cgctttctct tcatgatgcg taagcgcatg aaatttcccg tgaatcgatt gcaaatacgc
    14941 gcgtggccca tgcaactgga aattcgcact ttcgcctttg tcgagcacga tatggtgcag
    15001 ccacacttgc tggcgtaatt gcaggcttcc catcgtaccc tctggcgagg cgattaattg
    15061 ctgcttgccc atattaagcg ccagtttttg aatcagcgga ttctctcgct gcgggcaggc
    15121 gtccagccaa agctgcattc gtgttaacgg tttgtctttg ctgagattgt gttcgctgta
    15181 gctgacaccc ggctgggtag agagcagcaa cgcctcaccg gcgctggcct gaacatgatt
    15241 gccttcgctg tcgcgatact ctgcttctcc atccagaatc acatttaaaa tatcgacttt
    15301 gggataggtt cgcggctgaa aggcggcacc tggggccagc acttcctggt taagcacacg
    15361 cagggaggca tagcctaaca atttcgggtc gaagtagtgt ccaaaggaaa aagtataccg
    15421 ggcctgcaac catccgtagt ctgcttgtcc acactgtctg gcagttcggg tagtaatcat
    15481 aattcttgac ctctctttac ttccttttat ggtaaggggc tggacgctac attgttagcc
    15541 agatattctg cccggtatgt tcaaatttcc tgaatgagaa cgaaatggcc aaagaaaggg
    15601 cattaacgct ggaagcacta cgggttatgg atgcgatcga tcgccggggc agttttgcgg
    15661 cggcggcgga tgagctggga cgcgtgcctt ccgcacttag ctacaccatg caaaaactgg
    15721 aagaagagct ggatgtggtg ctgtttgacc gctcgggcca tcgcaccaaa ttcaccaatg
    15781 tcgggaggat gttgctggag cgagggcgcg ttttgctgga agccgcagat aaactgacta
    15841 ccgatgcgga agccctcgcg cgcggttggg aaacgcatct caccattgtg accgaggcgc
    15901 tggtaccgac acctgccttt ttcccgttaa tcgacaaact ggcggcaaaa gccaataccc
    15961 aactggcaat catcaccgaa gtgctggcgg gggcgtggga acgactggag caggggcggg
    16021 cggatattgt tatcgcgccg gatatgcatt ttcgttcctc gtcggagatc aactcgcgca
    16081 agctctatac cttaatgaac gtctacgttg ccgcgcctga tcacccgatt catcaggagc
    16141 cggaaccgtt atctgaagtg acgcgcgtga aatatcgtgg aattgcggta gcggataccg
    16201 ctcgtgagcg cccggtgttg accgtacagc tgctggacaa acagccgcgc ttaacggtga
    16261 gcacgattga agataaacgt caggcattac tggcggggct tggcgtggcg acgatgccgt
    16321 atccgatggt cgaaaaagat attgcggaag ggcggttgcg tgtcgtcagc ccggaatcga
    16381 ccagcgagat cgatattatt atggcctggc gtcgtgacag tatgggggaa gcgaaatcct
    16441 ggtgtctgcg ggaaattccc aaacttttta gcggaaaata atatttgtta ttattaataa
    16501 aaaggggcga aatgtcgccc caattctaag attaatttga accaaactta ggatcgtttc
    16561 cgtaacgatt gctgccagaa ttgccttcta aacaggcaaa tacaaacaga actaaccagc
    16621 caatgatcgg taccagatag agaagcgccc acgcacctga acggtcagta tcgtgcagac
    16681 gacgaacaca cagagcaatg accggaatga ttgttgctgc caggtaaatg agagaaagga
    16741 atggaaactc taaacctaaa atcaattgaa tgacattgat aatagcgccg acgatggcgt
    16801 taatcagagt aaacatccag tactctttac gacgtgcgcg accagagaaa ccaacataat
    16861 tttttaatac ggctaaatac cactgcataa aaacatccct ttaattaaaa gtgacgaaag
    16921 tctattccgg gtattgtgta aatgctattc gtatctttct ttttcggatg ttataaaggt
    16981 tttgatattt aagataaata catggttgtt ttttataggt aatttatata acgacattga
    17041 aatattttaa ataaacatta gtaaattatt cattcatcaa catgatgata aaagccggat
    17101 gagtcagaca tccggcgagc agatttgttt taaggttcca gtttcggatc tggtccaaag
    17161 cggttttcgc ctggcgtacc cgcctggcag ttaaagacga tgataataag ccagccgata
    17221 aacgggatta agaacaacag tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc
    17281 gtcaggatgc cttcgccacc agcgcgttgc cagcctaaca ttttatccag cagccccagc
    17341 acgaacgtaa agatgatgtt gaccagaata aacatccagt actctttgcg ccgcgcacga
    17401 ccccggaaac cggcataatt ttttagtact ttcagatacc agtccatttt tgcctcaatt
    17461 ttgctcaatc acttgttttt aaagcagcaa attaagaata gacgtgaatg aaatggcagt
    17521 aaatattcgt tcaaagaatt aataaagtgc tgtgaaggcg cgctaagact gatgacaagc
    17581 gcaaaatcgc cagatgcgct acgcttatct ggcctgcgcg gtcattgcaa catattgaat
    17641 ataaatgttt tgtaggtttg ataaggcgtt cacgccgcat ccgacatgaa caacatgcac
    17701 ttagctaatt atcagaggca cctcgtaagg tgcctttttt taaccgaagc gaacatctcg
    17761 tccatgcggt tcatcgagat cctgccatgg accaattgaa ataatccccg tagggttgat
    17821 ggtcttatgg ctgcggaagt aatgattacg gatgtgatcg aaattgactg tttcggcaat
    17881 tcccggcatc tggtagatat cacgcaggaa gccatacaga ttaaggtaat cgctgatgcg
    17941 gtgcttatca cacttgaagt gggtcacata cactggatca aaacggacca gcgtggtcca
    18001 caggcgaata tcggcttcgg ttagctggtt gccggtcagg taacgatgct ggcctaaaat
    18061 ctgctccagt cgcgccagcg attcaaacac tttcgccacc gcctcgtcgt aggcttgctg
    18121 gctggtggca aaaccagctt tatacacgcc gttgttaacg gtgtcataaa tccagccgtt
    18181 aagttcgtca attttcgttt gcagggctgg tgggtagtaa tcacccgctt tcgcgcccag
    18241 cgcatcaaac gcggtattaa acatgcggat gatttctgct gattcgttgc tgacgatggt
    18301 gtggttcttt ttgtcccaca gcacgggaac agtaactcgt ccgctgtagt gtggatcggc
    18361 gtggagataa agctgataca gaaattcatg ttgatagagc gtatctccgg ttgctcccgg
    18421 aaaactgtca tcaaaggtcc agccgttttc cagcatcagc gggttcacta cggaaacgga
    18481 aataaacggt tccagtcctt tgagtttgcg catgatcagc gtgcggtgcg cccacgggca
    18541 ggcgagtgaa acatagagat gataacgatc tttctctgcg ataaaaccgc ctgtgccagt
    18601 ggggccaggt gcgccatcgg cagtgagcca gttacgaaat gcggaagctg aacgttgaaa
    18661 tttaccgccg gtagatttgg tatcgtacca ggtgtcatgc cagacgccgt caatcagttg
    18721 acccattttt ctctcctcca atagcaaaaa cgaggagctt cctcctcgtt ttttatcgtt
    18781 aagtatagcg cgtgcttacc actttttatt cagcaggcgg tcgatgctat acgcgcccgg
    18841 accggtaatt gccagcagca ggaatccgcc agaaattgtc aggtttttca tgaacatcag
    18901 cgagttgacg ccttcagcaa agttgctgtg aaataaaaat gccgtcagca gcgtaaagcc
    18961 cgcagtaaac agggctgtgg tgcgagtcag gaaaccgaac aggattgcca gaccaccacc
    19021 gaactcaagc agaatcacca gcggcagcat aaaacccggg acacccattg cttccatata
    19081 ttgttgggta ccagagtaag cggtgatttt tccccagcct gcggtaataa acagaatcgg
    19141 cattaaaatg cgcgctacca gtacaccaac atcttctaat tttttcatca tactctccac
    19201 tgcgccactc aggctgctga ttgttttttt gtgcaagggc gcggtattag cgtcgttgct
    19261 gtcgatggag agaatcataa acgtggtgaa tgatgattgt tagcaaggaa aactgtcaaa
    19321 aatcttcaaa aaatttgagg gataaggccg gaattgctcc ggccagaggg aagttaaccg
    19381 cgaagctgtt gctgcttgag ggtcgttttt accagacgcc aggcgctcca tacgccaaaa
    19441 ccgcgtctgg cccagcggac cagcatatta ggatggcgaa tcgtccagat cgccatcacg
    19501 ctactgccaa ccagcgccca ggagcgcaga cttagcagca tattccagcg acgatcgtaa
    19561 gcgcctgttg cctccagcca ttcacgacga ctggcggaaa gatccagccg ttgttgctgg
    19621 atctggctaa gcagttgcgc cttacgtcgt tcacgttcga ctttactgct cacgggactc
    19681 ctcctcgagc agttgccgat cgtttgccag ctcatggcgt gtatggcgca gcaaggtgga
    19741 tttacgcgat ttacgtaacg tccagatacc gccaatcagt gccagtagca gcaacaccac
    19801 ggtggtggca atcatcgcat tcaggcgata ttgcggatca accgcccaaa taattagcac
    19861 catcaggctc ataagaccaa atgcagcgaa aagcatcgtc aggcccagca tcagtaaaag
    19921 ttgaaagaga ttagcttttt cctcttccag ctccaccacc gccagccgca gacgtgtttc
    19981 caccatttca accatgatgg aaacaattcg ctgcccgatg cccagaacgc ttttaccggg
    20041 cccttgcgcg tgatgagtgt ccgccataat taacgacgcg acagcagaac gccgagcact
    20101 acaccgattg cagcgccaat tcccacgccg gtccacggat tttcacgcac atactcatca
    20161 gcacgcgccg ccgcgacacg ggtttgtttg gcgatggcat caccggtttc acccaggcga
    20221 taacggctct gtttcagtgc ctgctccgct ttgctacgaa tcttactcaa ctcttctttc
    20281 gacttctcgc cagatgagct aagcacctct tccagcgtat cggaaaggga tttcaactca
    20341 gcacgcagat gttccgtagt gtgttctttc gacatagttt tctccaggtg agtaagtagt
    20401 gactattgtg agttagtagt cgcgcgcttc cagctttttc agctcttcct gcgcttctgc
    20461 cagtttccgt tcgcgtttgg caatcttatc ggcatcgcct ttttgcttcg cctcggctaa
    20521 atcttgctgg cgttccgcca cctcatcttt ctgctttgcg attttcttgt gatgatcggc
    20581 acgcagctgg ctatctgaac agttggcccg gacttcactc agggctttat tcagaccgtc
    20641 aatacgattc tggttttggt gtttttcggc atagctgatc tccttaagga tattttgctc
    20701 cttttcctga cacagggtag tggcataact accggcacta agagcaaaga gagaaacagc
    20761 taaagcgatg cggtatttca ttcttgaacc ttccattgtg attcggacct attacaaagg
    20821 aacgattgtc cagacaacga agcggtagtg ttaaccaccg cctcattaat catagacagt
    20881 tacggtgaaa tcaccaaagt gagtgatatg tttcggataa caggaagtta tccgaagcga
    20941 tgagagttat cccgtaaccg ggtcagccac tgcatagcct gactgttatt gtcctgctgc
    21001 gcaatgatgt agccatgggg caatgttctg tcgaggaccg cttttgcggc ggcgctctgg
    21061 tcgctggagt caaatgtaat taacagagtg tcgtttttgg gggtgatact tttgaaagga
    21121 atgccatgag cgtcaaggtg atgccagatc gaaaaaccgt ctggcatcgt tgtgccttga
    21181 tgaacggcac gaatcgccag cgtagactct tgctggcgaa ccgcggacca ggccagcaac
    21241 agcgtgccga ccagaagtaa aacagtgccg gaccaggcta gctggcgaag cgacatgcga
    21301 ggtatttgca tcccttaccc ccgatttcca tatttctttt tccataacac gaccagagaa
    21361 cctgccaggc caaacaccag cagcaccacc gggagcagca tcaggcatga catcagctgg
    21421 tcctcgtact ttaaaaatac cggcgttttg ccgagcatgt aacccagagt tgtcaggatc
    21481 aatacccaca gcagaccgct catccagttg aaaaactgaa agcgcgcgtt attcagccct
    21541 gataacccgg caatcgtcgg cagcagtgtt ctgacaaacg caataaagcg accaattaac
    21601 agcgccgata aaccgtgttt atgaaaaaga tggtgtgcgc gttgatgata atgcgcgggt
    21661 aaatgagata gccagttttg tacggtgcgg gtattgccca gccatcgccc ctgaatatag
    21721 ctgacccagc agccgaggct ggcggcaacg gtcagcagca gaatcgtttg cgggtaaccc
    21781 atcgcgcctt tcgcaatcaa cacgccgacc aataccagta aactgtcgcc cggtaaaaag
    21841 gccgctggaa gcaagccgtt ttcaaggaac aaaattacaa acaagacaaa atacaacatg
    21901 ccaatcatcg atggattggc cagggtttca aaatcctgcg cccacagggc ttgcagcaat
    21961 tgggtcaaaa gttccattca ttattcctgg tgaatcggtt aacgccacgc tggttttaaa
    22021 agatgcacta ccgcaatgtt attgctgttt tggtatgttc tgcgggtttg atacatcaac
    22081 acagcctgaa ctattccctg ttaaatcgtg agataagaaa gcattcaaaa acataaattc
    22141 aaattgaatc taattgtaac aaaggtcaac gttgttcgtt atcaaaatta catgtccgtg
    22201 gagtctgatt ttgctgaatg caggggaggg gggcgagagg atttacacag gctgacactt
    22261 tatacttttc gcttaccgac gagacaaaca ggcgcgtcgc agcgcctgtc ggacaggagt
    22321 ttcatttact gccactggtg gccgtatcga gatgcactac cgggttttcg gcgaacagat
    22381 agcggtcggc attgaactcg aagtcgtcgc tggtttcgtt gaataacatg atcttggtgt
    22441 tttccaggtg ctgccacatt gccagcttag cagcatgagg atctttgcga atcagcgcct
    22501 tgaggatttg atcgtggtca tcacaccagt tatcgacggt acgggaatca atgtgttcgt
    22561 gcagtttttt ccagtacggg ttatggctac gttgggtcca cattttttca acgatggccg
    22621 ccagggcgga gttctgcgtc gccagggcta cctgaatgtg gaactgtaaa tcccactcgg
    22681 aatcacggaa gcattgttcg ccgcgcgcct gttcctgaat ggccatcagt ttcatgatgt
    22741 cttgtttcgt tacctgagtt gccgcgaact cggcaatatt actttcgatg agctggcgag
    22801 cctgaagcaa ctcaaacgga ccgtaattgg cgaattccat attattgtca gccgcctgct
    22861 gatggcgcgg ctggttggaa accacatgaa tacctgaacc tttacgcact tcgacatagc
    22921 cttcaacttc cagcatgatg atggcttcac gaactaccgt acggctgacg ttcttttcat
    22981 cggcaataaa gcgttctgca ggcagtttat cacctaccag atagacgccc tgttcgatgc
    23041 gctctttcag gtcagcggca agttgttgat acaaacggcg tggttcagtg atttccatat
    23101 gcgtctcccg gagtggaatg cggacgaagt tatcatacca ctttattcat gggtatgcca
    23161 ttggtcatat ataaatttgg tgattgaatt aatgtattga attaatattg tgaatattta
    23221 taaattccta aaaaataagt atcgtcattg tgaaagctac taaatttcag taagttaatt
    23281 cccagccttt aaatttaata ttaactaaaa atgctttttt atattgactt acaataaatc
    23341 tacatctcta atacaccaac tttttggaaa caatattgta agatcaaatt tatatggtgg
    23401 cgaaaattaa tctcttatca cggcttacac cgcttttatt cgtgttcgct ccttttttgg
    23461 cacagtcaaa tatgactgtt tatcctatgg ctgtttccat taataatcag ggtgaaggca
    23521 acgttcgcgt aatatctaaa agtaatgaag ttcagtacat caaggcgaca gtattccgta
    23581 tcgataatcc atcgacgcct caggaaaatg aagttgagat taaatccggt gatgcaaatc
    23641 atctggttgt tatgccacct aaattcgctt taccagccgg tagtagcaaa accgtacgtt
    23701 ttgttgcgat ggaaccagag caaaaagaga aaaattatcg cgttaaattt gaagcggttc
    23761 ccagtattga tgacgttgcc acagataaaa aagatctctc tatgcagtta acagttaact
    23821 taatttgggg gattgttgtt agtgttccac ctcagcaacc tattgctaag ttagaagtaa
    23881 atgctgccca aaaattagtt aatgcaggga atcaacgctt aaagatttta acgatcgctt
    23941 attgtaaaaa taatagcaaa gaaaattgta agatacagac cgtaaataaa aacatcttcc
    24001 ctggtcagga aagaaatctt gaaagcatat ctggctacga caaaatcgtt gtcaaatata
    24061 ataactggat caccaaagat aatggcgagt ttgaactggc ggtccattaa ttatcattgt
    24121 aataaacgaa ggaatagtaa tgaaaaaggt atttgcaaaa tctcttctgg tcgcagcgat
    24181 gttttctgtt gcaggctccg cattggcagt acaaaaggat attactgtaa cggccaacgt
    24241 tgatgccgct ctggatatga cacagaccga taacaccgcg ttgccgaaag cagtagaaat
    24301 gcaatatctg ccggggcagg gtctgcaatc ttaccagttg atgaccaaaa tctggtctaa
    24361 cgacgttacc aaagatgtaa aaatgcagtt agtttccccg gcgcaactgg ttcaaagcct
    24421 ggatgctagc aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga
    24481 tgcggcaacc acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc
    24541 tctggctaaa aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta
    24601 ccgtggtgta gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca
    24661 cgtaacaaag ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt
    24721 tcaggattgc catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg
    24781 cagaggcgac attaaccaaa attcccgcag ggtttgaggt tattgctcag ggacagcagg
    24841 agtatatcga ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc
    24901 ttgataccgt aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag
    24961 acgatcagaa aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcgccacg
    25021 gtgaattggc ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata
    25081 cgctggaaat aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat
    25141 ggaattcagc tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg
    25201 cttttataca tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta
    25261 ttcagggaaa cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt
    25321 tcaacggtta tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt
    25381 atcgttatga ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac
    25441 tatttaatgc acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg
    25501 ggatgcgtat cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc
    25561 cagtaatggt tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt
    25621 tgggatcgtt ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag
    25681 gcagctacag cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg
    25741 tgccgtttac caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag
    25801 gtaaaactac atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc
    25861 tgccattaca tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg
    25921 ctttcgagtt aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca
    25981 gcgccagcgt gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga
    26041 gtaatccggg atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta
    26101 caaccgacag cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg
    26161 tttcacagaa ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca
    26221 ctgatgatag tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga
    26281 caacagcgca aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc
    26341 gtgactggat tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata
    26401 acgacgacaa cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca
    26461 ataacaacag ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg
    26521 atcaaacgtc atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac
    26581 ttggcgtaac cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt
    26641 gggatgggca atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc
    26701 atgatcatct ttcggccttt acgggcactt atagctctac gctggctgtc agtcgctatg
    26761 gcgttaattt gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga
    26821 aaggcttctc tgaacaggat gaagatagtc aggatctgca actcgaagcg cgggtggcag
    26881 gcagccgaac tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc
    26941 agtctggttt tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca
    27001 tcattaacgg tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg
    27061 aagtgtctgc cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga
    27121 aaaaattccc gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggcggat
    27181 ttacacttga aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc
    27241 ttaagtgtcc actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta
    27301 cttgtagtgt ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga
    27361 aacagcctaa attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa
    27421 tcgattgatt gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc
    27481 tgacgtgaca agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg
    27541 gaaggatgaa gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga
    27601 cctccaacaa acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga
    27661 acaacgcagt gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag
    27721 tagcgatgaa gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga
    27781 ttctaataga tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt
    27841 tcttacgctc taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa
    27901 ggggaaagtt aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct
    27961 gaatacgctc gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc
    28021 aagggtgcag ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga
    28081 tctgaccatg ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga
    28141 aatcatgctc aaagatgaag ggcgaccagc tgcagggcgc agagacggtt acttctctat
    28201 ctatcgtcag ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat
    28261 gtacaacccg gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa
    28321 cagcattaac ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat
    28381 gtgtgtgccg acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc
    28441 cgggtactac atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaatta
    28501 accctgcaaa ccggacaaac gcttttttct atataaacga cgaagccgtc tgataaggcg
    28561 gcttcgtcat tacaacttaa gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt
    28621 cctgcgccac ctcaatcgcc ggtttgttct gcaacacggt ccagataacc agcgcaccta
    28681 acaggtcaaa tactgccaga actgcgaaca gcgggctgaa gccgatggtg tcagccagtg
    28741 cacagacaac cagcgcaaac agcgtacttg ccagccatgc ggacatcccg gttaaaccgt
    28801 tcgccgtcgc cacttcgtta cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag
    28861 acagtgcctg gtgggcaaaa ccaccgatac acagcagcat aattgcgaca tacgggttgg
    28921 tgaacagacc gatcataccc gggccaatca tcagcacagc acccagcgtt acgaccatct
    28981 tacgggaaac gatcaggttc acaccaaacc aacgctggaa cagcggcggc aggtaaccac
    29041 cgaggataca accgaggtca gcaaacagca tcggcatcca ggcgaacatc gcgatctctt
    29101 tcaggttaaa gccgtaaact ttaaacatga acagcgggat ccacgcgtta aaagtacccc
    29161 aggccggttc tgccaggaaa cgcggcagcg cgatacccca gaactgacgg ttacgcagga
    29221 tctgaccaac ggacattttc ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa
    29281 taatatagtc gcgttcttca tcggtcagat gcttctggtc gcgtggatgt ttatagaaaa
    29341 tcagccatgc catcgcccag ataaagctca atgcaccgga gatgataaat gccatctgcc
    29401 agctgtgcat tacgattgcc cataccacca gcggcggcgc aatcatcgca ccaatcgaag
    29461 aacctacgtt aaagtaacct accgcgatgg aacgctcttt cgccgggaac cattcggagc
    29521 tggctttcag acccgccggg atcatcgctg cttccgcggc accgactgca ccacgagcaa
    29581 tcgccaggcc accccagcta ccagccagcg cggttgcacc acagaacacg gcccacagca
    29641 cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacatag cccgctaccg
    29701 gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg
    29761 agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt
    29821 aaccaagcac ggtgccaagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt
    29881 tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc
    29941 agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg
    30001 ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca
    30061 aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa
    30121 cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca
    30181 ttacctgacg acagcgaggc cagtactggc gcggcctgca gagagattta ccactttgag
    30241 agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa
    30301 attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg
    30361 aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc
    30421 cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg
    30481 ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc
    30541 gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt
    30601 gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc
    30661 aacccgttat accactggac gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa
    30721 ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag
    30781 gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc
    30841 gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc
    30901 atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca ggcgaccttt
    30961 aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac
    31021 ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct
    31081 gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact ggacagcatt
    31141 ctggcgcgcc gtctggctgg cgaacccctg agcgagcacg aagtggcaca gttcaaaact
    31201 gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac
    31261 attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc
    31321 tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct gagcaagcag
    31381 aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa
    31441 gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc
    31501 ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg
    31561 gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg
    31621 tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg
    31681 gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc
    31741 tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc
    31801 aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat ttggctgaag
    31861 gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt gctcgtggac
    31921 ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat ggcctgccga
    31981 ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac aatacgcgca
    32041 cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc
    32101 aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta
    32161 atgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg caaatccaga
    32221 accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc
    32281 acacctacgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa
    32341 acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca
    32401 accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc gttcatttca
    32461 tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg catcagctgt
    32521 ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc
    32581 tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt
    32641 tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt
    32701 ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg
    32761 tcaccatggt caacgacttc aaacagtact ttattgccca tgaccagccg atctatgaaa
    32821 acccatcgcc ggggaacaaa gcgggcggta tcaccacgct ggaagataaa tcccttggct
    32881 gtacccagaa agcgggttcc agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga
    32941 aaacgccggg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc
    33001 tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgtggcacg ccatacggcg
    33061 gctttgtacc gacggtgaaa atcgctacca acagtgaact ggcggcgaag aaaaaacact
    33121 ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag
    33181 aatttatcga caccatcgtt gtgtttgcca acggtaaaca aacctgcaac gagcgtaacg
    33241 acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt
    33301 taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgataagga
    33361 tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt
    33421 atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc
    33481 gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc
    33541 cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt
    33601 tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg
    33661 ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg
    33721 cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg
    33781 ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa tatcattcgt
    33841 ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc
    33901 gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt
    33961 ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc
    34021 aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg gcgatatcgt
    34081 tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc cccgccacgc
    34141 cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc gtgggcagat
    34201 cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    34261 cgccagccat attgatggtg gcacccagcg gaatagaaac ggaataggta tcgcgatcca
    34321 gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag ctgcgggtga
    34381 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    34441 gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa
    34501 ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg
    34561 tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa
    34621 aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca
    34681 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct ttcagcagcg
    34741 cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttcg acaatgcctg
    34801 acggcggcga aatatcaccc gcgctactgg ataaatgcag ggtagaaggg aaggcaaagc
    34861 tgaagactac tgcggccaga gcagcagaga aggtacccag cagatagagg aacaaaatag
    34921 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatc accagcatca
    34981 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    35041 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
    35101 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    35161 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
    35221 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    35281 gaagcgaaaa atgctggatt ttaaagagag gtcatatttt gcaacttaat ggttacattt
    35341 atttgacata aaaataaagc caacgtgcgg cttccagact gatgacaaca tcaaaaaagg
    35401 actcggtcag tcccctcgcc ccgtttgggg agagggttag ggtgagggga acaggccagc
    35461 atcggtgcga aaaattaccc tcaccccgac cctctccctg gaagggagag ggagaaaacg
    35521 gtgctcatgt taatgacagg gtacacggct taggcagatt aaaaattatc caccccgctg
    35581 cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca gaatgccaaa
    35641 caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat
    35701 accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac
    35761 gcccgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt ttgaggtcag
    35821 cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca cggcgaaaat
    35881 cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc
    35941 attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg
    36001 tagccagcgc accagcggct tgtctccaat gcctgattca tcttcatggg cgagcgccat
    36061 cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga
    36121 aattagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc
    36181 atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga acaacatcag
    36241 ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacctg tgagaaaggc
    36301 cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc accagaaagc
    36361 ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat
    36421 ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca gcataatggc
    36481 gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc
    36541 ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt atgcatagac
    36601 ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg tattcctttc
    36661 gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg accggcaatg
    36721 aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt caatggtctg
    36781 ttacatacct tttacactgt tttactttgg caattatagt tttacgctat ctgccggaaa
    36841 aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc gactgaccgc
    36901 cagccccgga tgatccacca gatgctcatc caccagggta gcgaacagct ctgcttcgta
    36961 gagcatggta ttaatatgct gcggctgggt gagatcctgc atttggctgc cacgcggcac
    37021 gaagcacact ttctggcatt cagacagttt ttcaatcacc agcgatcctg cttcgccctg
    37081 aatctcgctc gccaggacag aatcactgac ttttgagtgc tgcaaggtga cgctgaaatc
    37141 accgtaatcc atcaccacca cgccgtgggc atctacaccg cttgccagca aactggcggt
    37201 tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc agtaaaagcc
    37261 gatatccata attgaaccgt tagagaatga cggattaaag gtgttgggat tttcaccgtc
    37321 caggtaacgt tgatagcgcg aggaatactg gcaatagttg aaaaagactt tacgcaattt
    37381 gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc ggcaggcagg cggttttaaa
    37441 tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcac ccacttccgc
    37501 cagattcgac gccagtggtt tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg
    37561 tgtctgggaa aaatgcaggg cattcgggct ggcaatatac accgcgtcaa tggcatcgct
    37621 ttccgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg
    37681 ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac cgctctcatg
    37741 ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga aacgtatcat
    37801 aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt tttcatcttc
    37861 gacgtgcgca gatacgcatt attcttttgc cggacagcca tgctcactaa acggcccaat
    37921 catgaccgat ggcccgagat atttcggctg gcgatggagg attttggcgt caatcacggc
    37981 gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca tattgaggtc
    38041 aggcgcgtta aaaccgatag cgtcgatgct gtactgtttt gccagccaga tggcgcgttg
    38101 gttatgaaat tcttgcgaga taatggtgat atggttttca ccaaaaacct ttttggcacg
    38161 cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt tcgccggtac
    38221 gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt tacgtccgtt
    38281 atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg ctgcggtatc
    38341 aatacgtcgg gtaaagtagc gattacccgg cctggccccc agcaataagc ccacgttacg
    38401 ctccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga ccataacgcg
    38461 atcggcaata aaaatggtcg ctcctgctat aagcaacaac aaacagccaa tcttaagagt
    38521 ccggcgagaa aaacaaatac ggagaagaag gcggggctgc atggtcgtta tccttaacga
    38581 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    38641 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    38701 ggtggtactg cggctccata tggcagcaca actggtaaaa cgctttgtta tgctctttct
    38761 ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc
    38821 gaaacacagt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    38881 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    38941 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    39001 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    39061 ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg ggataaccct
    39121 gaagataagt aagattgttc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    39181 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    39241 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    39301 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    39361 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    39421 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    39481 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    39541 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    39601 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    39661 tgacattcac acttccacgc tggagctgtt cgagaaagtg ctcggcccga ccaccactac
    39721 gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc cgctggccga
    39781 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc
    39841 gaatgtcttc tcccgcaccg ggctggatat tggcgcacgc ttctttatgc aacatctgcc
    39901 agagaatctc gaaggtgaga ttgtcgatct cggctgtggt aacggcgtta ttggcctgac
    39961 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    40021 cgcttccagc cgaatgaatg ttgaaaccaa catgccagag gcgttggatc gctgcgagtt
    40081 tatgatcaac aacgcgctct ccggagtgga gcctttccgc tttaatgctg tgctctgcaa
    40141 cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    40201 cgcccgccgc tgcctgaaaa tcaacggcga actgtatatc gttgccaacc gtcacctgga
    40261 ttacttccat aaactgaaga agattttcgg caactgcacc accatcgcca cgaataataa
    40321 atttgtggtg ctgaaaacag tgaagctggg gcgtcgtcgg taagattggg agttacctgc
    40381 cggatgcggc gttaacgcct tatccggcct acgttcagca cgttactccc aggccggata
    40441 agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa
    40501 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttgtt caacgtttat
    40561 gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta
    40621 ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc
    40681 ggtggcacct tttctggcgg gtattcagaa cgaggaacaa tacgcccagg cgctggaact
    40741 ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgccaa
    40801 aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc
    40861 catgcctggc ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc
    40921 cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa
    40981 attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt
    41041 tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat
    41101 ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac
    41161 atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc
    41221 gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc
    41281 gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat
    41341 cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa
    41401 cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg
    41461 gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca
    41521 ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat
    41581 cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc
    41641 tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg
    41701 cggaacgcgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc
    41761 cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggtggta
    41821 atagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg
    41881 cccgccgatc tcgctgtggg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt
    41941 aatggcaaac gccagcccag caggtcccgc accgaccacc gccagatttt ttttctgcac
    42001 ggcaggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt
    42061 gactttgcca acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat
    42121 ctcatcggct cgccccgatt gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg
    42181 tgccatcgac accatatcgg catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc
    42241 gttaatccgg ttggtggtca ccagcggcag cgaaacgtgg cctttcagtt tgcgcgtgac
    42301 ccaagtaaat gcgccgcgcg gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca
    42361 gccaatgccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagctctac
    42421 cgtttcggca aaagtcccgc cgtcttcgac caggtcgagc atcgacagtc ggtagataat
    42481 aatgaagtcg ttgccgacgc gttcgcgcac cgcacgcact acttctacgg caaatcgcat
    42541 ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa
    42601 ttcgttgatc aaataccctt cggaccccat tacctctaca ccgtcgtatc ctgcctcccg
    42661 tgccagttgc gcgcagtggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag
    42721 ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gagggagcga ccagatgcgg
    42781 ttggtagctg tagcgcccgg tatgcaaaat ttgcagggct attttgccgc cttcctgatg
    42841 taccgcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc
    42901 accttccatg ccaacgcctg ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac
    42961 gccgtgacgg gcgcgttcgg cataaaacgc tgccagccgc tcggcaccgt ccgggtattc
    43021 ctccagcccg gtgtgcattg agcccatcaa cacgcggttt tttaacgtgg taaaacctaa
    43081 atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg
    43141 ttatgtggtc ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgc
    43201 ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg
    43261 ccgcgtttta tccggcctgg gggattgcac atgtagggcg gataaggcgt tcacgccgca
    43321 tccgccagtg gcacggtgca gatgccggat gcgacgcttg tcgcgtttta tccggccagg
    43381 aggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    43441 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    43501 ttggtgcgcc ttgctgtgcg ccagtcagcg ggttgtaatt ctcctgaatt gggccatcgg
    43561 cggttaatcc tttggcgtga cggaagaacg tatccgccag tttcagggca tcatcgcgat
    43621 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac
    43681 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    43741 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggca ttggcctgcg
    43801 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg
    43861 gtttgcccgc gcagccgttc gccagtggtt tatcttcaat acgtacgtca tagtagaact
    43921 gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc
    43981 gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt
    44041 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    44101 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc
    44161 cattagcgac atatttatcc aactgttctt tgtcgataaa accaaagacg gcggcatcat
    44221 cacgccccga ttcccacgac gcagcgacct gtgccggaat ttccagactg tcgtactggc
    44281 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttgttgcctt
    44341 tcttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    44401 catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    44461 ccaccagttt cgggtacatc tctgccaacc aggctttatc ctgggtgacg ttgtacactt
    44521 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    44581 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    44641 gcggacgcac gctatcgcca ggctggatct gccaggagaa gaccgcgcgg atattctctt
    44701 tggcgatgtc gggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    44761 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtaacggtg ttaaatttca
    44821 ccgcaccgcc cggtgagcgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    44881 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcaggtat tcttcccagc
    44941 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    45001 gctctttgct aacttcctga gcggtcagca gatgggaata ggtggtgtag agcgtggtcg
    45061 aaccgttgat atgcgcctta ctggtaaagc gattaccgtt gatttcagtc tgcaccggca
    45121 gggatttatg cacctgatat tccgattcgc cggaggtcag cagatcccag gtggcgcgca
    45181 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tgatagtcag
    45241 ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    45301 ccagcagttc gccgtcccac accagatcca gcggtttatt gctggtgatt ttggtttcca
    45361 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacttgcaca tcttttgctg
    45421 tcagtttttg caccagcgca ccgggaatac tgtatgcctc cagcgtaaag tcgactttct
    45481 tgccgtcctg ccagacggtc aggcggtcga aattgctggc cataaagttg atgtactctt
    45541 ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt agggccgtct ggcaacagat
    45601 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctggtgatcg tcgtaatcgt
    45661 aatccttcat gtactgcggc gcgccagtac ggttaatcac gtttttataa ttgtcggcgt
    45721 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    45781 ttattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    45841 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgtggcgaaa
    45901 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    45961 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaac
    46021 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    46081 gcgcgtccgg tatggtgcca ggcgcgagcg gcaaagttag gagttagcag gcccgtgaga
    46141 tagagctgtc ctgtgccgtc ggtgatttcc agaccgttca tccaggtgat gccttcggca
    46201 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcatagcga
    46261 tcgtacttgc caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg
    46321 gtgacgctat aacgaaagtt gccgcttaag ttctgcacct tcagcgcggt cataatgtcg
    46381 cgggtattgg gaatgacgta gcccagatcg ggggtgaagt cgccccacca ttgcaggtca
    46441 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    46501 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc tggcacccag
    46561 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    46621 gagccgtact tcatctccag gccaccttca atataggtac cgtcgctgta tttatgttta
    46681 ttctcaccct ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa
    46741 ggagaagatg tcgaggtaat tgcaggtgtt tttgtgtcgt catgttctgc cggacgtgct
    46801 tcttctcctg ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt
    46861 gcagtgatta atttcattgt gttaatccat gagctaaaga aaaagtacag ccttttatcg
    46921 ttgtaattat aaaaatataa aggcagaaac tattttcccc ctccccatcc aggaagaggg
    46981 aaaatgcctg ctcatttttt attagtctgc tcaatcaaca ttgctggctg gcgtcgcttc
    47041 aatatggcgc tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa
    47101 gccgaggaag ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc
    47161 agtcgggaat gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac
    47221 gataataccg gtgcggcggg agcccatgcg gaaatcgcgt ggcaggtgat ctaatttggc
    47281 gcgtaaattc aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcagt
    47341 catattaata atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat
    47401 catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgctggaa cgccgttttc
    47461 gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt
    47521 caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc
    47581 gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac
    47641 ctgcaccgat ccgccggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac
    47701 cgaggagacg gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa
    47761 cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc
    47821 ggccataaag atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc
    47881 gtcaacggtg atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa
    47941 aatgtaggag agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccaa
    48001 cattttcgcc ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat
    48061 ggtggtggca accggcgtca taatatattc gtagccgagg aaggcatacg aagcataagc
    48121 aataacgcgc ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc
    48181 agtaataaat gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc
    48241 tgagttttta tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa
    48301 aatcgtcgcg aggaaaaaca tcggtgccga ggccagtcca agctcaatat tattattgat
    48361 aacgttgtta aagctgtaaa cggcggcaaa agtcagcgag agcaagccga atttgccgat
    48421 tgtattacgt ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg
    48481 gcaaactcct tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg
    48541 actttgagaa ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata
    48601 tcgcaaataa cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat
    48661 tcacggtcag tttcatcacg ataatattcc actacctgta atgtctcttt cggcgcatat
    48721 tcaatttttt gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta
    48781 aatagcgcat cggtcgcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag
    48841 tgggcgacgc caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac
    48901 ttcttgccag aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct
    48961 tattctcgct gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc
    49021 tttgcgcggt agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac
    49081 ggaaccagac gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa
    49141 gcagctggtg gtcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct
    49201 gggcagcgtg gatgttttcc tgggtatagc gccaggcgct gaagttaatt gggcgctgcg
    49261 gaaccaccag cagaccgttg ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac
    49321 ggttgccgtt gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc
    49381 agatatcgat gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt
    49441 aatacgccac ctgatcgtat tcgccgttaa tccccatggt gaaaccgatg cacggaatga
    49501 tgtgcggata gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag
    49561 cgatgcgcca gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg
    49621 tgcggctgat gatcaacact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt
    49681 cctgcatgat ctgcaaatga ttcggttgcc acagcccttc gtactcctgc ttgtggttgt
    49741 cgatcattgg cttgaagaag ttgatctttg gctcgcgggt cagcagcgac tcgccattca
    49801 cctgccagga tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc
    49861 gaacggtgca gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga
    49921 aaggcactgg ttgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc
    49981 cggcttcgct gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc
    50041 gggcgtccag ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc
    50101 gcagtttaat ctgctgtgtc gcgagcgttt caccttcgac gcgcacctct gcgtgcaggg
    50161 tgtagtcatc aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgtgagtca
    50221 gatccagcgc gtggattttt accggcgcga taacctgttt gtactctttc aggcccggtc
    50281 ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt
    50341 cgccgtagtc gccgccgaat ttataccaga cgttgccgtt gtcatcctgc gcctggatcc
    50401 cgtggtcgca ccattcccag acataatgtc cttgaatgca atcgtgctta tagaagacgt
    50461 tctggtactc cgtcagcccg cctggtccgt tccccatcgc atgagcatat tcacagatga
    50521 tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca
    50581 tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg
    50641 tgtcatccag cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt
    50701 cattgcccag cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa
    50761 caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc
    50821 cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac aggccgtaga
    50881 tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga
    50941 tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc
    51001 ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga
    51061 tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt
    51121 ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt
    51181 ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt
    51241 caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat
    51301 attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa
    51361 gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg
    51421 ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgctgaccac cacatatcct
    51481 ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac aggttgtcgc
    51541 cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac
    51601 ccacatactg accgttaaca tagacttcaa aataggtttc gacgccgtca aatttaatca
    51661 gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg
    51721 ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt
    51781 gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag
    51841 ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat
    51901 tccactgacc gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt
    51961 gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga
    52021 gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca
    52081 tggtgatggt agtttgtgta attgctgggg agtttagtaa aatttttact aaaaagtagc
    52141 aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg tccggcaaaa
    52201 acgattgccg gacagtgtgt aaggggattt aacgggtcgt gccgcgcagt tttaatttgc
    52261 tgggaacgaa gactaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt
    52321 taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg
    52381 taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca
    52441 ggcctcgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa gcaacaaaca
    52501 gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa cccgacgaac
    52561 tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt
    52621 ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg ccaataaaac
    52681 caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg atgcgtgcca
    52741 gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat
    52801 tgtcggtcaa cgcacaggcg gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa
    52861 taccggtgac gtttttaatg tctggtaatc cgctgtgttc ataacagttg gtgagctcaa
    52921 tgcccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag taaggatcgt
    52981 tgatctccag ctcctgttgg tagctgtaga tagccagaat atggtgttgg ttgactgcac
    53041 ctgtctggag tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa
    53101 tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata
    53161 ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct
    53221 tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt
    53281 ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt
    53341 gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt
    53401 tagtaaaatt ttacccgaag ggaggaaaca atggaaaccg tggcttacgc tgattttgca
    53461 cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag
    53521 ctgtacatcg tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg
    53581 ccgtactaca gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    53641 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    53701 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    53761 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    53821 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    53881 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    53941 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    54001 aactcaattg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    54061 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    54121 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    54181 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    54241 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg
    54301 atatccggct gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc
    54361 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg
    54421 gtgagatagc ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc
    54481 accgcagcca catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg
    54541 atattgccaa acggcacatg acggaagccc ggcagcagtg gcataaacgg tttgcggaag
    54601 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    54661 gcaataaaag taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct
    54721 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    54781 gcagcaaggg ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc
    54841 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    54901 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    54961 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    55021 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    55081 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    55141 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    55201 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    55261 gcgaaaatcg ggataaacac aaattcggtt atcatttgtg caatatttaa tgctatctgt
    55321 taacatttgt tgttattttg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    55381 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    55441 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    55501 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    55561 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    55621 tcacccagca aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc
    55681 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    55741 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    55801 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    55861 gcaaaaatgg tgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    55921 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg
    55981 agccgctgta caaagcgctg aacgccggac gtaccagtaa gcgtattcat aaaggcctgg
    56041 tggtgcgtaa aggctggctg ggtaaactgc cttcattacc gcttcgctgg cgggcgcgtg
    56101 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    56161 tggtgacgta tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc
    56221 agattgtgcg cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc
    56281 gtaatagtgt tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag
    56341 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    56401 tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc
    56461 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    56521 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    56581 cagtgcaggg tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca
    56641 gtacccagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    56701 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    56761 cagtggtggc aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    56821 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg
    56881 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    56941 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    57001 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    57061 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    57121 aatcggtatg ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt
    57181 atcaggcctg cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    57241 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    57301 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg
    57361 ttgacgtgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    57421 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt
    57481 tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca gcgcaatctg cttcttacca
    57541 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgttgggtag
    57601 atgacgcccg ggcttggggt gtaattcccc tgggttaggt tctcaatcgc tttaatcaat
    57661 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    57721 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga
    57781 ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    57841 cagccctcat ggcgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata
    57901 tcagcctcct tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca
    57961 agtacaaaaa tgatgtttaa acgtggaagc acctcacatc caggccggat aagacgttca
    58021 cgccgcatcc ggcagcattg ctatggcact gaatgccttc acatacttca gatatatcag
    58081 ttctacaaat cgcttgcatt tatcatgatt aacaatcatt atcatttacg aaaattattt
    58141 agatatatct gattactatc acgaaggcga taacaatgaa taactccccc cgctacccgc
    58201 agcgcgttcg caatgatctg cgcttccgtg aactgactgt gttacgcgtt gagcgcatca
    58261 gcgccggttt tcagcgcatt gtcctcggcg gcgaggcgct ggacggtttt acatcgcgcg
    58321 gctttgacga tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa
    58381 cggtaacgga agagggcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata
    58441 cgccgctgta tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg
    58501 gggtcgccag cggctgggcg atgcaggcgc aaccgggcga taaacttacg gtggcaggtc
    58561 cgcgcggttc gctggtggtg ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat
    58621 ccggaatgcc cgcactgcgc cgccgcctgg aaacgttgag caaacttgcc gttaaaccgc
    58681 aagttagtgc gctggttagc gtgcgggata acgcctgtca ggattatctc gcgcaccttg
    58741 atggttttaa tatcgaatgg ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc
    58801 agatgcaaat ccctgccgat gattacttca tctggataac cggcgaaggt aaagtcgtta
    58861 agaatttaag ccgccgcttt gaagcggaac agtatgaccc acagcgggta cgtgcagcgg
    58921 cttactggca cgcaaagtaa ctgatatata aaaagaaaaa ggctgacgat ttctcgtcag
    58981 cctttgctat atctggtggc ccctgctgga cttgaaccag cgaccaagcg attatgagtt
    59041 ggttgataac cctttactta cagatactta gcgatacttt tcaatacctt agcatttttt
    59101 aaaactactg tataaatacc tgtatatacc ccaaaatact tctttacggt atcctatagg
    59161 tatcctggag cagaacaagc gattttccag gataccagat cgcttggtgg ggtgtatgag
    59221 tgaaattatt cagattctct aaagtaaaaa ttgcagaact gccaacgccg gaacatggac
    59281 aggtcgaata tggtgatacg gaggttaacg ggctgcgtgt ccgtgtaggt tcgtctggtg
    59341 tgaaaagttt ctgcatttcc cgtaagcgta acggaaaatt tattcgtgca acgctggggc
    59401 gttttcctga tctgagcgtg gataatgcca gggctaaagc tatggaactc cttggcgaag
    59461 tcgccacaac ggggataaat ccgaacatag caaagcgcac taacaacaag gccatgataa
    59521 cgctgagtga ggcgattgac gcctacgtaa aaaatcgtgg ccaccgcctg aaaccctcca
    59581 cagaaaaaca gtaccgcagc atattacaga attattccgg tgactggatg gcccattccc
    59641 tcgccagtat ttcccgcgaa cgggtggaac aacggcataa ggccatcact gatggggctg
    59701 tatggttcgg tgctgataaa accacgttac gggctggggt tggtacagga agcaaggcac
    59761 aggccgattt atgggcgcgt gcgctacgtg ctgtgtgtcg gtttgcgcat gatcattacc
    59821 gtgatgatgc gggtaatact cttttaccgg atccgccgac tctggtactg agcaccaagc
    59881 gccagtggca cggaacagta agaaaaacgg aacgtatccg cctgcatgat tttccccgct
    59941 ggtttgctgc tgtagctgct gtagctgctg tgcgcgacca gggcgaacag gaaagggatg
    60001 atatagctgt tacggtatgt gatgccgtgg aaatggcact gtttaccgga ctgaggaaat
    60061 cagaagtttt tggccttacg tgggatcgcg tgaatatggg cggtcgcttt ttctggatag
    60121 acaccacgaa aaacggggat ccgcttgaac tgccgatcac tgatacgttg cggaaaatgt
    60181 ttcttcgcag gctaaaaaac aaagctgctg atgatgtgct ggtgtttcct ggcgtgaagg
    60241 gagtgattca ggagacacgg cacatcatag accggataag cgcggctacc attcctgagg
    60301 cgaacgatga aatgctcttc cctgtcccgt ttaagtggca cgacgcacgc cggacgttcg
    60361 gaacgattgc tgagctggtg ggcgtgggta attacatcct caagcgcctg atgaatcacc
    60421 gcactatgcg aagtgccgac gtaacgcagg ggtatttaca tttcggtgct gatgaactgc
    60481 tggagccagc atcacggata gaacgggcaa ttctggaaca tgctgggctg gtggagagta
    60541 aaaaatctat cgatgcgcaa ttactttccg cactggaggg catgagtgat gatgaaaaac
    60601 gtcgtttact atttgctata gtacaagaaa agaatgacat atatgggtga taacaatata
    60661 tcgg
//
LOCUS       DAHBHC010000020        58883 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHBHC010000020 DAHBHC010000000
VERSION     DAHBHC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.020"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..332)
                     /locus_tag="M2A35_001624"
     CDS             complement(<1..332)
                     /locus_tag="M2A35_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCB7197939.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGG"
     gene            complement(329..1999)
                     /gene="menD"
                     /locus_tag="M2A35_001625"
     CDS             complement(329..1999)
                     /gene="menD"
                     /locus_tag="M2A35_001625"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCB7197940.1"
                     /translation="MSVSAFYRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2088..3383)
                     /gene="menF"
                     /locus_tag="M2A35_001626"
     CDS             complement(2088..3383)
                     /gene="menF"
                     /locus_tag="M2A35_001626"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCB7197941.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3462..3767)
                     /gene="elaB"
                     /locus_tag="M2A35_001627"
     CDS             complement(3462..3767)
                     /gene="elaB"
                     /locus_tag="M2A35_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCB7197942.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3822..4283)
                     /locus_tag="M2A35_001628"
     CDS             complement(3822..4283)
                     /locus_tag="M2A35_001628"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7197943.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4348..5265
                     /gene="rbn"
                     /locus_tag="M2A35_001629"
     CDS             4348..5265
                     /gene="rbn"
                     /locus_tag="M2A35_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCB7197944.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5453..6673
                     /gene="elaD"
                     /locus_tag="M2A35_001630"
     CDS             5453..6673
                     /gene="elaD"
                     /locus_tag="M2A35_001630"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCB7197945.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDESNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLTRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            7085..7698
                     /locus_tag="M2A35_001631"
                     /pseudo
     CDS             7085..7698
                     /locus_tag="M2A35_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7801..8304
                     /locus_tag="M2A35_001632"
     CDS             7801..8304
                     /locus_tag="M2A35_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCB7197946.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(8371..9828)
                     /gene="nuoN"
                     /locus_tag="M2A35_001633"
     CDS             complement(8371..9828)
                     /gene="nuoN"
                     /locus_tag="M2A35_001633"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCB7197947.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(9835..11364)
                     /gene="nuoM"
                     /locus_tag="M2A35_001634"
     CDS             complement(9835..11364)
                     /gene="nuoM"
                     /locus_tag="M2A35_001634"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCB7197948.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(11528..13369)
                     /gene="nuoL"
                     /locus_tag="M2A35_001635"
     CDS             complement(11528..13369)
                     /gene="nuoL"
                     /locus_tag="M2A35_001635"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCB7197949.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(13366..13668)
                     /gene="nuoK"
                     /locus_tag="M2A35_001636"
     CDS             complement(13366..13668)
                     /gene="nuoK"
                     /locus_tag="M2A35_001636"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCB7197950.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(13665..14219)
                     /gene="nuoJ"
                     /locus_tag="M2A35_001637"
     CDS             complement(13665..14219)
                     /gene="nuoJ"
                     /locus_tag="M2A35_001637"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCB7197951.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(14231..14773)
                     /gene="nuoI"
                     /locus_tag="M2A35_001638"
     CDS             complement(14231..14773)
                     /gene="nuoI"
                     /locus_tag="M2A35_001638"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCB7197952.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(14788..15765)
                     /gene="nuoH"
                     /locus_tag="M2A35_001639"
     CDS             complement(14788..15765)
                     /gene="nuoH"
                     /locus_tag="M2A35_001639"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCB7197953.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(15762..18488)
                     /gene="nuoG"
                     /locus_tag="M2A35_001640"
     CDS             complement(15762..18488)
                     /gene="nuoG"
                     /locus_tag="M2A35_001640"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCB7197954.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(18541..19878)
                     /gene="nuoF"
                     /locus_tag="M2A35_001641"
     CDS             complement(18541..19878)
                     /gene="nuoF"
                     /locus_tag="M2A35_001641"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCB7197955.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(19875..20375)
                     /gene="nuoE"
                     /locus_tag="M2A35_001642"
     CDS             complement(19875..20375)
                     /gene="nuoE"
                     /locus_tag="M2A35_001642"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCB7197956.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(20378..22180)
                     /gene="nuoC"
                     /locus_tag="M2A35_001643"
     CDS             complement(20378..22180)
                     /gene="nuoC"
                     /locus_tag="M2A35_001643"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCB7197957.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(22274..22936)
                     /gene="nuoB"
                     /locus_tag="M2A35_001644"
     CDS             complement(22274..22936)
                     /gene="nuoB"
                     /locus_tag="M2A35_001644"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCB7197958.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(22952..23395)
                     /gene="nuoA"
                     /locus_tag="M2A35_001645"
     CDS             complement(22952..23395)
                     /gene="nuoA"
                     /locus_tag="M2A35_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCB7197959.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(24026..24964)
                     /gene="lrhA"
                     /locus_tag="M2A35_001646"
     CDS             complement(24026..24964)
                     /gene="lrhA"
                     /locus_tag="M2A35_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCB7197960.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            25884..27101
                     /gene="alaA"
                     /locus_tag="M2A35_001647"
     CDS             25884..27101
                     /gene="alaA"
                     /locus_tag="M2A35_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCB7197961.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            27185..27784
                     /gene="yfbR"
                     /locus_tag="M2A35_001648"
     CDS             27185..27784
                     /gene="yfbR"
                     /locus_tag="M2A35_001648"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCB7197962.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(27843..29675)
                     /locus_tag="M2A35_001649"
     CDS             complement(27843..29675)
                     /locus_tag="M2A35_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCB7197963.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(29762..30412)
                     /gene="hxpA"
                     /locus_tag="M2A35_001650"
     CDS             complement(29762..30412)
                     /gene="hxpA"
                     /locus_tag="M2A35_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCB7197964.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(30423..30917)
                     /locus_tag="M2A35_001651"
     CDS             complement(30423..30917)
                     /locus_tag="M2A35_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCB7197965.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(31000..31455)
                     /locus_tag="M2A35_001652"
     CDS             complement(31000..31455)
                     /locus_tag="M2A35_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCB7197966.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            31793..32995
                     /gene="ackA"
                     /locus_tag="M2A35_001653"
     CDS             31793..32995
                     /gene="ackA"
                     /locus_tag="M2A35_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCB7197967.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            33070..35214
                     /gene="pta"
                     /locus_tag="M2A35_001654"
     CDS             33070..35214
                     /gene="pta"
                     /locus_tag="M2A35_001654"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCB7197968.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            35404..36924
                     /gene="yfcC"
                     /locus_tag="M2A35_001655"
     CDS             35404..36924
                     /gene="yfcC"
                     /locus_tag="M2A35_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCB7197969.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(36957..37499)
                     /gene="yfcD"
                     /locus_tag="M2A35_001656"
     CDS             complement(36957..37499)
                     /gene="yfcD"
                     /locus_tag="M2A35_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCB7197970.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(37557..38108)
                     /gene="yfcE"
                     /locus_tag="M2A35_001657"
     CDS             complement(37557..38108)
                     /gene="yfcE"
                     /locus_tag="M2A35_001657"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCB7197971.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(38164..38808)
                     /gene="yfcF"
                     /locus_tag="M2A35_001658"
     CDS             complement(38164..38808)
                     /gene="yfcF"
                     /locus_tag="M2A35_001658"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCB7197972.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            38944..39591
                     /gene="yfcG"
                     /locus_tag="M2A35_001659"
     CDS             38944..39591
                     /gene="yfcG"
                     /locus_tag="M2A35_001659"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCB7197973.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            39648..40010
                     /gene="folX"
                     /locus_tag="M2A35_001660"
     CDS             39648..40010
                     /gene="folX"
                     /locus_tag="M2A35_001660"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCB7197974.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            40031..40924
                     /locus_tag="M2A35_001661"
     CDS             40031..40924
                     /locus_tag="M2A35_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCB7197975.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(40972..41862)
                     /gene="rpnB"
                     /locus_tag="M2A35_001662"
     CDS             complement(40972..41862)
                     /gene="rpnB"
                     /locus_tag="M2A35_001662"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCB7197976.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(42059..42832)
                     /gene="hisP"
                     /locus_tag="M2A35_001663"
     CDS             complement(42059..42832)
                     /gene="hisP"
                     /locus_tag="M2A35_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCB7197977.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(42840..43556)
                     /locus_tag="M2A35_001664"
     CDS             complement(42840..43556)
                     /locus_tag="M2A35_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7197978.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(43553..44239)
                     /gene="hisQ"
                     /locus_tag="M2A35_001665"
     CDS             complement(43553..44239)
                     /gene="hisQ"
                     /locus_tag="M2A35_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCB7197979.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(44329..45111)
                     /gene="hisJ"
                     /locus_tag="M2A35_001666"
     CDS             complement(44329..45111)
                     /gene="hisJ"
                     /locus_tag="M2A35_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCB7197980.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(45332..46114)
                     /gene="argT"
                     /locus_tag="M2A35_001667"
     CDS             complement(45332..46114)
                     /gene="argT"
                     /locus_tag="M2A35_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCB7197981.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(46380..46949)
                     /gene="ubiX"
                     /locus_tag="M2A35_001668"
     CDS             complement(46380..46949)
                     /gene="ubiX"
                     /locus_tag="M2A35_001668"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCB7197982.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(47044..48561)
                     /gene="purF"
                     /locus_tag="M2A35_001669"
     CDS             complement(47044..48561)
                     /gene="purF"
                     /locus_tag="M2A35_001669"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCB7197983.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(48598..49086)
                     /gene="cvpA"
                     /locus_tag="M2A35_001670"
     CDS             complement(48598..49086)
                     /gene="cvpA"
                     /locus_tag="M2A35_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCB7197984.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(49345..50007)
                     /gene="dedD"
                     /locus_tag="M2A35_001671"
     CDS             complement(49345..50007)
                     /gene="dedD"
                     /locus_tag="M2A35_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCB7197985.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(49997..51265)
                     /gene="folC"
                     /locus_tag="M2A35_001672"
     CDS             complement(49997..51265)
                     /gene="folC"
                     /locus_tag="M2A35_001672"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCB7197986.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(51335..52249)
                     /gene="accD"
                     /locus_tag="M2A35_001673"
     CDS             complement(51335..52249)
                     /gene="accD"
                     /locus_tag="M2A35_001673"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCB7197987.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(52405..53064)
                     /locus_tag="M2A35_001674"
     CDS             complement(52405..53064)
                     /locus_tag="M2A35_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7197988.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(53147..53959)
                     /gene="truA"
                     /locus_tag="M2A35_001675"
     CDS             complement(53147..53959)
                     /gene="truA"
                     /locus_tag="M2A35_001675"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCB7197989.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(53959..54972)
                     /locus_tag="M2A35_001676"
     CDS             complement(53959..54972)
                     /locus_tag="M2A35_001676"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCB7197990.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(55038..56195)
                     /gene="pdxB"
                     /locus_tag="M2A35_001677"
     CDS             complement(55038..56195)
                     /gene="pdxB"
                     /locus_tag="M2A35_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCB7197991.1"
                     /translation="MSETEKGLKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(56354..57358)
                     /locus_tag="M2A35_001678"
     CDS             complement(56354..57358)
                     /locus_tag="M2A35_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7197992.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFDKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWMYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSQKIAEGLKPSSANRYMNRLSGMFTVLKKIGLWDAEHPVRGISILYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWSEACKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTRETGGLFEVKYREFCLALKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQKILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHAVSTGD
                     "
     gene            complement(57455..57775)
                     /locus_tag="M2A35_001679"
     CDS             complement(57455..57775)
                     /locus_tag="M2A35_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7197993.1"
                     /translation="MASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEVRGK
                     QVTEGALLKVTKHPKFKKYAYWLSTDETMPEVGQISPALSLDGSCNSEGDRVSTETIQ
                     KLSR"
     gene            57889..58176
                     /locus_tag="M2A35_001680"
     CDS             57889..58176
                     /locus_tag="M2A35_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197994.1"
                     /translation="MSESELGGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNEGMRKAFFEQPKERRDAWLLWLGL"
     gene            58183..58389
                     /locus_tag="M2A35_001681"
     CDS             58183..58389
                     /locus_tag="M2A35_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197995.1"
                     /translation="MTSHIISAILTLVFIEIGVIAIYLFRKLTGHEERFIELSIEYIA
                     AYTKGLFPAAIGAMFIAFVIWFIG"
     gene            58642..>58883
                     /locus_tag="M2A35_001682"
     CDS             58642..>58883
                     /locus_tag="M2A35_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCB7197996.1"
                     /translation="MKQQRNSRFRNGAERHANRFATSASRSNIRYSLSDTHATPDGHT
                     VKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALSCG"
BASE COUNT        14857 a        15997 c        14365 g        13664 t
ORIGIN      
        1 ccgccttcga caataacccc acaaagcccc gccagcccct ggcaagccgc catcatcgcc
       61 acccgtccgc caagcgagta ccccaccagc caaaagtcaa ggatgttgta actaaccaag
      121 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
      181 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
      241 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
      301 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
      361 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
      421 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
      481 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
      541 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
      601 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
      661 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
      721 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
      781 aacctgctgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa
      841 acaattgccc ctgttcaggc agatagtcgc agatgcgatg cgccagttgc gcttcgccaa
      901 acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
      961 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
     1021 ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc
     1081 agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttgccc gtcaggctgc
     1141 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
     1201 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
     1261 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
     1321 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
     1381 ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
     1441 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
     1501 tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
     1561 aacgtgcggg gatatcccgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg
     1621 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
     1681 gatcggcggt taacaggatc agtttttctc cggttaaccc ggcttcaatc agtgcaggat
     1741 agagatttgc taccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
     1801 tcgccagccc cagcgccaga tgccccaacc cacgctcatc gaaatgggtg tggtgaatga
     1861 atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcca ggggcgatac
     1921 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
     1981 aaaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
     2041 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt
     2101 aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
     2161 gaaccacgga caatgcccgc gccagcatat agccgcacga cattgccgct gatttttgct
     2221 gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
     2281 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
     2341 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
     2401 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
     2461 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
     2521 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc
     2581 gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt acgcagcgct
     2641 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca
     2701 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
     2761 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
     2821 agctcccctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
     2881 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
     2941 accagtgtgg cgataaattc tcttgcctga atcgcatcgt gctgaaggga gctttcgctg
     3001 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcgtggtaaa
     3061 agtaaattgc cctgcgacgg gtcaaacgca ttcagccccc aaatgcgtaa gtcggcgtgt
     3121 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
     3181 cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
     3241 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
     3301 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
     3361 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
     3421 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
     3481 agtcctagta ccagcccgac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg
     3541 acgtagtcat cagcacgata aacagcctgc ttcgcccgat agtaataact gtctgaagcc
     3601 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
     3661 tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc
     3721 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
     3781 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
     3841 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
     3901 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
     3961 ggccagtgat gcgtgcaggt gtcgagggtt ttactcatca gttgctgccc cactttttcg
     4021 ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca
     4081 cttttcagaa tcctcgcata cgccaccagt tcatcatttt tccaaccgag aatatggcga
     4141 ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca
     4201 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
     4261 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg
     4321 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcagccggt
     4381 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag
     4441 agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
     4501 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
     4561 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
     4621 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
     4681 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
     4741 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
     4801 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
     4861 ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag
     4921 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
     4981 gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac
     5041 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
     5101 cgccaggctg cgacactagc ccgtgaggct ggagtcggta agctaatcat tacccacgtc
     5161 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
     5221 ccggcgactg aactagcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta
     5281 aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc acccacacgg
     5341 gcatattgag cgatttttga tatttattca tctccttacg cactatattc agagcaataa
     5401 gttgcctctt tttatcttca ttcagcacaa cctttgcaac taaaaggagc ccatgatggt
     5461 tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga catttgcaga
     5521 aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga
     5581 tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa
     5641 agctgaagat attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat
     5701 ccgacttatt aaaaatgcct gtttaaaatt atataatttg gcacataccg caaccaactc
     5761 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
     5821 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
     5881 gctttcaaag gtgcttttaa atgatgagag taatgaactg agtgatgata caaacttctg
     5941 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
     6001 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
     6061 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
     6121 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
     6181 agtaaataaa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
     6241 actaggccat gataaagaaa attcacttac gcgtgtcatt aatagtttta attctgaact
     6301 catgggagaa atgtcgaata ataatataaa agttcattta aatgaaccag agataatatt
     6361 tttacatgcc gatcttcagc aatacttaag ccaaagttgc ggtgcgtttg tgtgcatggc
     6421 agcccaggaa gtgattgaac aaagggaaag caattctgac agcgccccct atacgttatt
     6481 aaaaaactat gctgacagat ttaaaaaata ttcagcagaa gagcagtacg aaattgattt
     6541 tcaacatcga ctggtaaaca gaaattgtta tttagataaa tatggcgatg cacgaatcaa
     6601 tgcttcttat acacaattag aaataaaaca ctcacaaccc aaaaatagag catccggcaa
     6661 aagagtgagt taacatcatt gttaattaat tcgaccagtc agcaaatatg ctgactggtc
     6721 gaacatagtt tgctaaacag tcatcactga ctgatgtcag tcacaccatc cgccagttca
     6781 atcttcgggt atttccagcg tatctcggca cgcgattgct ggtctgttga tgtagtggat
     6841 tgttgttgct gacttttctt accctcagag ttctgccccc tgtcaactca atctgccaat
     6901 aaataacata attatatttt tccttatatg aattcctttt taaaatagcg aagccactgt
     6961 aaaagatact ttttgtatag atctaacaac cgtaataaaa caaacactca taactcatta
     7021 caaataatga taaaataacg tcttttattg ataatttata tatgaaaaaa aatccttttg
     7081 ttatattatt tctgatttca ctccctctga ttatttttac caaccctggg tcactgcatt
     7141 atcgccaacg ccccgacacg tcagtcctga gcaattagaa aaaacagttc gttatcttac
     7201 acaaactgtg catccacgta atgccgacaa tattgataat ctgaataggt ccgtagagta
     7261 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     7321 gctattacga ctctgcgcct ggtagccagg attaccctta cccgcaatgt cctggcttta
     7381 tcccgatcgg ggggacttta ttgcctggtc ggcagaatac aggatatcaa cgccgttcgt
     7441 caggtaaaaa cggcattgtt atcatcccgg gatttatctg tttattctat gaatgcccca
     7501 tggtttattc ccggtattga tttctctgac cacctgaatt attggcaaca cgatattccc
     7561 gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat
     7621 acagcagaca gattgaatta tcagaaaatg gctcaggtgg tggatggggt tataactttg
     7681 ttatacaaca gtaaataata aaaatttctc gaggcgggtc cagaaaaagc gacatgcaca
     7741 ttttattttc cctcgtttat tttaagatgc gaaacatgaa attcaacgga ggaaaaataa
     7801 atgggaatga ttggctattt tgcagaaatt gactcagaaa aaattaacca actgcttgag
     7861 tctactgaaa aacccttaat ggacaatatt catgacactc tttcaggatt acgccgactg
     7921 gatattgata aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat
     7981 cccgcgaaga atgatccgct gagtcgtgcc gtgcttggtg aacacagcct cgaagatggc
     8041 atagatggct tcctgggatt gacctggaat caggagttag ccgcgacaat cgaccgactg
     8101 gaagctttgg atcgaagtga gctgcgtaaa aagttttcca ttaagcgact caatgaaatg
     8161 gagatttatc cgggagtgac cttttcagaa gagttagagg ggcaactctt tgcttcgatc
     8221 atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca ggggaatcat
     8281 gcgctgacgg ttattgttgg gtgatggtgt cagagcgcca aaaaaaaccg ccgaacatgt
     8341 cggcggcttt ctgaattacg aactgacagc ttacatcagc ggcatggcta accgcacaat
     8401 gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag agatcagcac
     8461 aacgataccg cccgcgctgt actgccagtt tgatggtgca tcgcgacccg gttgttccgg
     8521 ggcgtgaaga tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc
     8581 gacaaccacg gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta
     8641 gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt
     8701 catcactgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc
     8761 agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct
     8821 gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccggtttg
     8881 cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag
     8941 acgtttgatg ttggtctggc tcaacgccat caggttaccg aagatgatgg aggcaaaggc
     9001 gataatcgcc agtactacgc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag
     9061 acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg
     9121 cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt
     9181 gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct cgttgagcat
     9241 accgtcgccg aggtttttac ccaacgcgac aaacgacagg tcgccagact gcgcatacac
     9301 cagcgccata ccgaacagca ggaatgaaga cgctgcggca gaaaggatgg tgtatttgat
     9361 actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa
     9421 agagatcagt tcaataccga ggaacagaga cgccagatgg ttggcattcg ccagcaggat
     9481 cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc
     9541 ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt
     9601 gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg
     9661 gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt
     9721 gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac
     9781 gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg
     9841 ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga
     9901 ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag
     9961 gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt
    10021 cgctttaccg aagtaagcgc gatgtaacat cgccagcgaa taaacagatg caaagaccag
    10081 cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat
    10141 aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag
    10201 cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg
    10261 ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg ccgacaagcc
    10321 gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagatggc
    10381 aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatgtcggt
    10441 ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc acatagcgat
    10501 tggcgcaaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca gcaaaccgta
    10561 agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg gagcctggga
    10621 gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg cgaaggcgat
    10681 gaagaagccc agcattaaca ggtattccac accactggac attggcgtat tcagcagctc
    10741 ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat
    10801 ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt
    10861 gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg
    10921 caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc
    10981 gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat
    11041 ctctttccac gaacagagta ccgccagcac accgagcaga ccggtcagca cgaccatcag
    11101 cagcgacagc ccgtcaatgg cgagatgaat agagatacca aaacgcggga tccacggcat
    11161 gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg
    11221 caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg
    11281 cggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccga taaagggaat
    11341 taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac
    11401 cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat
    11461 gcaccgcgaa tgcggcttat ccgacctacg ttcttatatg cccaggggca atcccacaat
    11521 ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg
    11581 atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta cctgcaaagc
    11641 gggaaaggac agccgggatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg
    11701 caatacccag gaatggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt
    11761 tgtaccacca ggtgcccagc agacggcccg gcgcactgtt ggcgatggag gtcaccagag
    11821 tacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacgccag
    11881 aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc
    11941 cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca
    12001 gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga
    12061 agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccc accagacctg
    12121 ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga
    12181 agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga
    12241 gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat
    12301 ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag aacgcgtgag
    12361 tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc
    12421 caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg
    12481 caaaaccggc cagcagcagc gtaaccgccc caacaatgcc caccagatgc agaacttccg
    12541 gggtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg
    12601 ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtca gcaagccatg
    12661 tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc
    12721 acatcagcat gttattgcca tcagcaaagt gcgctggtgc cagttccacc atttcgcgga
    12781 agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg aaaacgtcac
    12841 ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga tcggtgtaat
    12901 agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg tacatcagca
    12961 gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg gtgtaggcga
    13021 agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tggatcagga
    13081 aacccacacc agtgaccact gagagcatgg tcagcgacag gccgtccaac accaggttaa
    13141 aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg tatgcctgct
    13201 cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc aggcccacag
    13261 agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag aacgccagca
    13321 ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc
    13381 acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag
    13441 gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc
    13501 ggtctgaccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat
    13561 ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag
    13621 aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc
    13681 gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca
    13741 cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc
    13801 gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg
    13861 tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag catgatggcc
    13921 gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt
    13981 tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat aatggcaccc
    14041 gcgtagacga taatttccag cgcaccggcg aagtaagcgc ctagtgagaa gaacaccccg
    14101 gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg
    14161 atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt
    14221 gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt
    14281 cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt
    14341 atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt
    14401 attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc
    14461 acagaccaca gaaaatgcag cgtgagaagt tgatacggaa aaattccgga taccagcgac
    14521 cgtctttggt ttctgccttt tgcagcgaga tacagccgac cgggcaggct accgcacaga
    14581 ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac
    14641 gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg cgttttgcga
    14701 acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt
    14761 cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt
    14821 taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg
    14881 gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc
    14941 ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa
    15001 cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca cgaagaacag
    15061 accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc
    15121 cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa
    15181 accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat
    15241 attgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga acacttcgta
    15301 gctcagggtc tgcgcagaag cacgcatcgc accgagcagc gagtatttgt tgttacttga
    15361 ccagcccgca aacagcaccg cgtaaactgc cagacctgcc atcatcagga agaacaaaat
    15421 cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaattg caaaggccag
    15481 cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaactt
    15541 cgggatccag tcttctttaa agaacatttt gatcatatcc gcaaccaact ggagcgaacc
    15601 gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc
    15661 gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca ccgctttgag
    15721 gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg
    15781 agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaag
    15841 cccacctgcc ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac cgtgttgcca
    15901 tcgtaactaa aggagacgcg tgtacctgcg tttacaccca acttcgcggc atccgctggg
    15961 ttgagtttga tgtacggctg cggcatacga ctctggaaga ccggagcacg ctgtgacaat
    16021 tcatcactgc caaacaggtg gtagtacggc gcaatacgcc atttcccgtc ctgcggctgg
    16081 aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc aaacagacgc
    16141 acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc
    16201 tgcggggagt tccagcccgg agcccaggca aacggcactt gcgaacggtg cgcggtcggc
    16261 tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg ctgacgcggc
    16321 tcatgaacgc tgatattggc gcgcatggcg gtacggccgc tgtaacggtg cggttcacgg
    16381 gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacctgcc
    16441 agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact
    16501 tcacggctca gcagggtgct gtgcagcgag tgtaaccaac gccagctttc cagcatgaca
    16561 gttttgctat cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca
    16621 ttattaatta ccgtaccgtc gctttcagca aagctggcag cggaaagcac cagatgggcg
    16681 ttttccatga tcgctgtgcg ttgatgatca accaccatca ccagcggcgc tttagccagc
    16741 gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag caccaccacc
    16801 gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc gccacccata
    16861 atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac gtcagcgccg
    16921 cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctaa gctaccggcg
    16981 ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac gatgacgtcg
    17041 attttgcttt gcagctcagg ttcgataccg tcaaccgctg gtgcagagtt atccagcgca
    17101 tgggcaatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc
    17161 gcgatatcat ccagacgagt gtcatcaacg ttggtaacaa acagcggatg cttagcacgt
    17221 tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt
    17281 tcgcgcgctt tacctttcac cgcctgacgc actgccagcg cgacgcgcgc gccggtctgg
    17341 gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt caatttcgcg cagagccgga
    17401 gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg
    17461 tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttgctt
    17521 tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg cagaatatct
    17581 gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt
    17641 actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg accacggtcg
    17701 cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg
    17761 taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca
    17821 aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg
    17881 gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg
    17941 cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc gtcagcgtaa
    18001 tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg
    18061 aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg
    18121 tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga
    18181 cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct
    18241 tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc
    18301 aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca
    18361 ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga
    18421 caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt
    18481 gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat cgaaattcgg
    18541 ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa
    18601 cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg
    18661 ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag
    18721 ttgctcaagt gtttcgatat ctcccggctg accttcgcca cgctccagcg cacgcagaat
    18781 tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc
    18841 gaaaaactct tccagattac gcaccagcga caccatgttg atctcatggt caaccgccat
    18901 cgccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag
    18961 gtcaaggtgc gcttcagtca ggaaatcagt ccccgcgccg cctggctgcc aggctttgaa
    19021 tttcagaccg tcacgcatac caccggcgta atcttcaagg atttcacgcg cggtagtacc
    19081 gaacggcagt tcccacaggc ccggattttt cacccgacca gagaagccca tcagcttggt
    19141 gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc
    19201 cggaacgtta cacagggttt cgacgttgtt gacgcaggtt ggtttacccc atgcaccgga
    19261 ggttgccggg aaaggtggct tcgagcgtgg gttagcgcga cgcccttcca gggagttgat
    19321 taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa
    19381 gtcaaaacct gttcccataa tatttttgcc aagcaaacct gcttcggtgg cttcggcaat
    19441 ggcacggcgc agattcactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg
    19501 gtaagctttc agcgcaaacg cggagataag cataccttcc accagcaggt gcggcagttg
    19561 ctccatcaga aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag
    19621 gtaacggatg ttcatggatt cgtctttcgg catcaggctc catttcaggc cggtagaaaa
    19681 gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg
    19741 agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc gatattcgtc
    19801 cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt
    19861 acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga
    19921 tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt ccccaggcag
    19981 caagttggca gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt gatgtttagc
    20041 tttttctcga gcgccgcctg aataccctgg taaccgttga tatgacagac cacgctgtcg
    20101 caataacgga tcacatggcg gccaaccggc tggcggaaga tctggctgta gaacgttgcc
    20161 acgccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcgcca
    20221 tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc
    20281 gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc actcagctca
    20341 aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa
    20401 caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg
    20461 ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga taactcatgg
    20521 tgctaccgtc gctggtcagg tagtaactgt tgatcccctt cgtcgcctca atcatctgga
    20581 aagattcatt ggcaggcatc accggccccc acgacacttg caagaagtgg gtgatcaggg
    20641 tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg tgatccgctt
    20701 tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca
    20761 gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga
    20821 tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga
    20881 tcccggtggc acgcaggccc gcgccggtgg tgccccactc cagcgcctct ttcgcgccat
    20941 aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgct gctttctcgt
    21001 aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccac
    21061 gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata cggaaaccag
    21121 tgattgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag aacactggcg
    21181 tcattgcgcc gacgtcctga ataaaggtcg agatatacag caggtggctg ttgatgcgga
    21241 acagctcaga aagcattacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca
    21301 gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcgatac
    21361 ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc tccgcaccac
    21421 ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccatcg agctgcaaaa
    21481 cgatacggaa agcaccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt
    21541 cctcgttttc agtaccacgc ttcatccccc actcttccgg tttgaaggtc agggcttcca
    21601 tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg
    21661 ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt
    21721 tcgggtgacc gtcgaatgta atgccaaaca gatcccaggt ttcacgctca taccagttag
    21781 cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct
    21841 tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg
    21901 cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca
    21961 gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac
    22021 gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc
    22081 caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg
    22141 cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac
    22201 tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc
    22261 cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac
    22321 cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca
    22381 cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc agtgcctgca
    22441 tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat
    22501 cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca caggcaccca
    22561 ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca
    22621 ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggg gaagcacgca
    22681 atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa
    22741 cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa
    22801 ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg
    22861 ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg
    22921 tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg
    22981 tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac
    23041 gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc
    23101 ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt
    23161 cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg
    23221 agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac
    23281 cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa
    23341 agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt
    23401 actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt
    23461 catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt
    23521 gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca
    23581 ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca gatccttgac
    23641 gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac
    23701 gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga
    23761 tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt
    23821 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgtacgg ggcgtgaatc
    23881 tctggtgatt gaaaaatgaa cagttttgta cgttctgcac gatgtgaaga aggttcttta
    23941 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc
    24001 tcaggaatta tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat
    24061 ccccttccgg agcagatatt gggctgtatt gccacggatt atggtagctc tccatcgcct
    24121 gatagatcac ctgagccagc tcattattac tggacgggtc atagcacagc aaatattcgg
    24181 tatcgggtag cggcggtaag ccatcgactc cactcagcac acgcaggtct ggactcatca
    24241 tctcgaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa
    24301 gcgtcgaggc gacataagcc agtcgccagg gaatatctgc tttattaagc gtcgccagca
    24361 ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc tcaccttttt
    24421 gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct
    24481 taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt
    24541 ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac
    24601 tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag
    24661 ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca
    24721 ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac
    24781 cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta
    24841 cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa
    24901 cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta
    24961 tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa
    25021 caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt
    25081 aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa
    25141 gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta
    25201 tttatgacat gcgcgtgttt gtataaatgt aaatgtaagt ccttgttcca ctctcgtgca
    25261 gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaggcggca tcaagagcag
    25321 ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc
    25381 gaattatctg atgcaaagtt atgttatgtt aggcaaagta atcttctatt attaataagc
    25441 acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc attcattaat
    25501 tccgcttaac aatagtttca caccttgccc ccattgcgac acctcggaaa tcatcacgct
    25561 gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa ttcttaaatg
    25621 aggcattttc acacaattat cttacagata aaaaaaccag acttacaatt aagaatcaga
    25681 acaattcact atataacatt gcatgtaaag catatacacc tcattatttt atcattatta
    25741 agttattaac agcacaatcg agccttccac tctggcaaaa tcttattctg cagaccttca
    25801 aaacaccgtc ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat
    25861 aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg
    25921 tctgttatga catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta
    25981 acaaggtact gaaactgaac atcggcaacc cagccccgtt cggttttgac gcgccagatg
    26041 aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca agggtattgc gattccaaag
    26101 ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg
    26161 ttaccgtgga agatatttac atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc
    26221 aggcattgct gaacagcggg gacgaaatgt tggttcctgc accagattac ccactatgga
    26281 ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg
    26341 actggttccc ggacctcgat gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg
    26401 ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga
    26461 ttgtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa
    26521 ttctctacga cgacgctgag catcactcaa ttgcgccgct ggcacctgac ctgctgacca
    26581 ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg
    26641 tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt
    26701 caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt
    26761 atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg
    26821 cgtgggaact gatcaacgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt
    26881 atatgttccc gaaaatcgac gccaaacgct ttaacattca cgacgatcag aaaatggtgc
    26941 tggatttcct gttgcaggaa aaagtcctgt tggtgcaagg gacggcattc aactggccgt
    27001 ggccggatca cttccgcatt gtcactctgc cgcgtgtcga tgatatcgag ctgtctttga
    27061 gtaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga
    27121 gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt
    27181 cactatgaaa cagagccatt tttttgccca tctctcacgc ctgaaactca ttaaccgctg
    27241 gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat
    27301 ggtcgcccat gcgctggcag ctatcaaaaa tcgtaaattt ggcggtaatg tcaacgccga
    27361 acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc
    27421 tactccggtg aaatacttca attcgcaaat cgctcaggag tacaaggcta ttgaaaaaat
    27481 cgctcagcaa aaactggtcg atatggttcc ggaagagctg caggatatct ttgcgccgtt
    27541 aattgacgag catgcatata gcgatgaaga aaaatcgttg gtgaaacagg cagatgcact
    27601 gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga aataatgaat tcttgctggc
    27661 aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc caggagatgg actacttcat
    27721 ggaagtattt gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct
    27781 gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc cggatgcgac atcaggctct
    27841 tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg
    27901 aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagt
    27961 gtgttaaccg gtgaagaaac cggtgtcata aaggcggcag atgctgccat cgccacgacc
    28021 atcgcgaatg gataaggcga cacccccatc gttttggcag cagccagcgc aatcggagcc
    28081 atcaacaccg ccgtcgcggt attagagata aacagcccaa taaccgccga caaaacaaac
    28141 aaacacccca gcatcatatg tggcccgtaa ccgccgccaa tgtccatcag ccctttcacc
    28201 gccagcgcga cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc
    28261 aaaataatgc tcggccagtg aatggattta taggcgcttt cggcatcgat acagcggaat
    28321 ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc atcggtcagc
    28381 attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctggct gtgcgcgggc
    28441 gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgttttgcc
    28501 agcataccga tcagtttcca gttaccaaca accaggatga tatcgcccag cagcagaggc
    28561 tcatccgcca gcgaaccttc cagcgccacg ccattgcgct ttagccccac cacattcagt
    28621 ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caatcagttc tgactcagga
    28681 attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc
    28741 agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca
    28801 gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg
    28861 ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcgcc ataacgctca
    28921 cgtaatttga gatcgtccag ccgttgacca atcattggcg atccggggcg aatagccaga
    28981 cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt tcgacgcgtc
    29041 cagccttcgc gctgcggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata
    29101 tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag
    29161 ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc
    29221 agcgtcatca tgccgctaat cagcccggca aaacttaacg gcatcatcag acgtgacggc
    29281 gaagtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgccg
    29341 gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg
    29401 atttcactgt tgcccgccac tttgaccagc catgttccca ttacggtggc aacaccggta
    29461 cggaccaaac catcgccaat aataaacaag gcggcaatca ggacaacgtt aggatcagaa
    29521 aagccggaaa atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca
    29581 aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc
    29641 agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg
    29701 atgacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt
    29761 ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt
    29821 ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga
    29881 catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc accaccacac
    29941 actcctgcgg cgcaagcccc agcagctgcg cacctaacag atacgcatca ggttctggtt
    30001 ttccgcgctt cactcgctca gcggttacaa acacctctgg tgcgggaagc ccagctattt
    30061 tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg
    30121 ctttattcaa atgattgagt aaggcgattg cccccggaag cgcggtaata ccttcggttt
    30181 ccgtggcctc gatgtgctcc agacgcgtaa actcggcggc aatatcagcc tcggatttgc
    30241 ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct
    30301 cttccggtgc taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca
    30361 gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acacgggcct
    30421 cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag
    30481 gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa
    30541 ccgtgagttc cggcgtcaaa atgggtatag cgcccttcca cattaaccat aaagcggaca
    30601 taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca
    30661 tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg atacatctcc
    30721 ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccaat
    30781 tcacgcatct gtaatccgta accacgctca ataattgttt gcaggcgacg gtaacgttca
    30841 gcatttgccg gatcgagcat agtcatcatc ttgtactgat tagacaaaat aagacgttgc
    30901 gcgttggtca tttccattgt tgactcctgt atcactatac tacggtgaaa aaaaagaagg
    30961 ctgagtatgc cttcttttat atgcgtaatc aggggtcaat tacaaatcat caaggaaagt
    31021 tttatccagt tgtttgaagg cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt
    31081 gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa
    31141 ccagttaagg attgcagggg gtaatggcgt tacagaacgc ttgcccagcc accacaatcc
    31201 ctgcatgggt aaacttaagg cgaacagcgc agtggcaact gccggcccaa gctgaccgcc
    31261 cagggcaatc tgccagcaga gagtaaatac ggcgatcggc ggcataaaac ggatcgcata
    31321 acgcgtcatc ttgataacgc gattttcgac aaagaccggg gcaaggcgtt tttccagcgg
    31381 ccacgtcttt gagtaatgct gtccccggcg aaacaagcta aaaaaattaa cagaacgatt
    31441 atccggcgtt gacatgcttc acctcaactt cacatataaa gattcaaaaa tttgtgcaaa
    31501 ttcacaactc agcgggacaa cgttcaaaac attttgtctt ccatacccac tatcaggtat
    31561 cctttagcag cctgaaggcc taagtagtac atattcattg agtcgtcaaa ttcatataca
    31621 ttatgccatt ggctgaaaat tacgcaaaat ggcatagact caagatattt ctttcatcat
    31681 gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc atgcgctacg
    31741 ctctatggct ccctgacgtt tttttagcca cgtatcaatt ataggtactt ccatgtcgag
    31801 taagttagta ctggttctga actgcggtag ttcttcactg aaatttgcca tcatcgatgc
    31861 agtaaatggt gaagagtacc tttctggttt agccgaatgt ttccacctgc ccgaagcacg
    31921 tatcaaatgg aaaatggacg gcaataaaca ggaagcggct ttaggtgcag gcgccgctca
    31981 cagcgaagcg ctcaacttta tcgttaatac tattctggca caaaaaccag aactgtctgc
    32041 gcagctgact gctatcggtc accgtatcgt acacggcggc gaaaagtata ccagctccgt
    32101 agtgatcgat gagtctgtta ttcagggtat caaagatgca gcttcttttg caccgctgca
    32161 caacccggct cacctgatcg gtatcgaaga agctctgaaa tctttcccac agctgaaaga
    32221 caaaaacgtt gctgtatttg acaccgcgtt ccaccagact atgccggaag agtcttacct
    32281 ctacgccctg ccgtacaacc tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg
    32341 caccagccac ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga
    32401 actgaacatc atcacctgcc acctgggcaa cggtggttcc gtttctgcta tccgcaacgg
    32461 taaatgcgtt gacacctcta tgggcctgac cccgctggaa ggtctggtca tgggtacccg
    32521 ttctggtgat atcgatccgg cgatcatctt ccacctgcac gacaccctgg gcatgagcgt
    32581 tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac
    32641 cagcgactgc cgctatgttg aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat
    32701 ggacgtttac tgccaccgcc tggcgaaata catcggtgcc tacactgcgc tgatggatgg
    32761 tcgtctggac gctgttgtat tcactggtgg tatcggtgaa aatgccgcga tggttcgtga
    32821 actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc
    32881 tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg cggtggttat
    32941 cccaaccaac gaagaactgg ttatcgcgca agacgcgagc cgcctgactg cctgatttca
    33001 caccgccagc tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga
    33061 ggataaaccg tgtcccgtat tattatgctg atccctaccg gaaccagcgt cggtctgacc
    33121 agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc
    33181 aaacctatcg ctcagccgcg taccggtggc gatgcgcccg atcagactac gactatcgtg
    33241 cgtgcgaact cttccaccac gacggccgct gaaccgctga aaatgagcta cgttgaaggt
    33301 ctgctttcca gcaatcagaa agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct
    33361 aacaccaaag acgctgaagt cgttctggtt gaaggtctgg tcccgacacg taagcaccag
    33421 tttgcccagt ctctgaacta cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt
    33481 atgtctcagg gcactgacac cccggaacag ctgaaagagc gtatcgaact gacccgcaac
    33541 agcttcggcg gtgccaaaaa caccaacatc accggcgtta tcgttaacaa actgaacgca
    33601 ccggttgatg aacagggtcg tactcgcccg gatctgtccg agattttcga cgactcttcc
    33661 aaagctaaag taaacaatgt tgatccggcg aagctgcaag aatccagccc gctgccggtt
    33721 ctcggcgctg tgccgtggag ctttgacctg atcgcgactc gtgcgatcga tatggctcgc
    33781 cacctgaatg cgaccatcat caacgaaggc gacatcaata ctcgccgcgt taaatccgtc
    33841 actttctgcg cacgcagcat tccgcacatg ctggagcact tccgtgccgg ttctctgctg
    33901 gtgacttccg cagaccgtcc tgacgtgctg gtggccgctt gcctggcagc catgaacggc
    33961 gtagaaatcg gtgccctgct gctgactggc ggctacgaaa tggacgcgcg catttctaaa
    34021 ctgtgcgaac gtgctttcgc taccggcctg ccggtattta tggtgaacac caacacctgg
    34081 cagacctctc tgagcctgca gagcttcaac ctggaagttc cggttgacga tcacgagcgt
    34141 atcgagaaag ttcaggaata cgttgctaac tacatcaacg ctgactggat cgaatctctg
    34201 actgccactt ctgagcgcag ccgtcgtctg tctccgcctg cgttccgtta tcagctgact
    34261 gaacttgcgc gcaaagcggg caaacgtatc gtactgccgg aaggtgacga accgcgtacc
    34321 gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat
    34381 ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag aactgggtgc agggattgaa
    34441 atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag
    34501 aacaaaggca tgaccgaaac cgttgcccgc gaacagttgg aagacaacgt ggtgctcggt
    34561 acgctgatgc tggaacagga tgaagttgat ggtctggttt ccggtgctgt tcacactacc
    34621 gcaaacacca tccgtccgcc gctgcagctg atcaaaactg caccgggcag ctccctggta
    34681 tcttccgtgt tcttcatgct gctgccggaa caggtttacg tttacggtga ctgtgcgatc
    34741 aacccggatc cgaccgctga acagctggca gaaatcgcga ttcagtccgc tgattccgct
    34801 gcggccttcg gtatcgaacc gcgcgttgct atgctctcct actccaccgg tacttctggt
    34861 gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct
    34921 gacctgatga tcgacggtcc gctgcagtac gacgctgcgg taatggctga cgttgcgaaa
    34981 tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg tgttcatctt cccggatctg
    35041 aacaccggta acaccaccta caaagcggta cagcgttctg ccgacctgat ctccatcggg
    35101 ccgatgctgc agggtatgcg caagccggtt aacgacctgt cccgtggcgc actggttgac
    35161 gatatcgtct acaccatcgc gctgactgcg attcagtctg cacagcagca gtaatctcgt
    35221 catcatccgc agctttgcgc tgcggatatc tgaaccggaa ataatcacta tttccggttt
    35281 tttattctct taatttgcat taatcctttc tgattatctt gcttaactgc gctgcatcaa
    35341 tgaattgcgc catctcactt tgcatactta ccactttgtt ttgtgcaagg gaatatttgc
    35401 gctatgtccg caatcactga atccaaacca acaagaagat gggcaatgcc cgatacgttg
    35461 gtgattatct tttttgttgc tattttaacc agccttgcca cctgggtagt tccggtgggg
    35521 atgtttgaca gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc
    35581 gtagatccac actcatttcg cattctgact aacgaagcag gcgaacctga gtatcaccgc
    35641 gtacagctgt tcacgacggg cgatgaacgc ccgggcctga tgaacttccc gtttgaagga
    35701 ttaacctcag gatcgaaata cgggacagcc gttggcatca tcatgtttat gctggtgatt
    35761 ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg ataacggtat cctggcgctt
    35821 attcgccata cccgcgggaa tgaaattctc tttattcctg cgctgtttat tctgttttca
    35881 cttggcggcg cggtatttgg tatgggagaa gaggccgtcg cctttgccat tatcatcgca
    35941 ccgctaatgg tccggctggg ctatgacagt attaccaccg tcctggtgac ctatattgcc
    36001 acgcaaatcg gttttgccag ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg
    36061 attgccggcg ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc
    36121 actctgattg gcctgatctt taccatggtg tacgcctcac gagtgaaaaa gaatcctctt
    36181 ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa agcaggctga tgttgaacaa
    36241 cgtccgttta cctttggtga ctggctggta ttgattgtcc tgaccgccgt aatggtctgg
    36301 gtgatttggg gcgtgatcgt taatgcctgg tttattccag aaattgccag ccagttcttc
    36361 accatgggtc tggtgattgg catcatcggc gttgttttcc gccttaacgg catgacggtt
    36421 aataccatgg cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg
    36481 gtgggtttcg ccaaagggat tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc
    36541 gtgttaaata ccatcctcaa cagcattgcc aatgccatta gcggtctgga caacgcggtc
    36601 gcggcctggt ttatgttgct cttccaggcg gtatttaatt tcttcgtgac gtccggttct
    36661 ggtcaggcgg cgttaaccat gccgttactg gcaccgcttg gcgatctggt cggtgttaac
    36721 cgccaggtta ccgtgctggc attccagttt ggtgatggct tcagtcacat catttaccca
    36781 acctcagctt cgttaatggc aactctcggt gtatgcaggg tggacttccg taactggctg
    36841 aaggtgggcg cgaccctact tggactgctg tttattatgt ccagcgtcgt agtgatcggc
    36901 gctcagttga tgggctacca ctaaaaatgt taagagccgc aattgcggct cttttttcat
    36961 tctgccgttt cagtctctac ggcttcattt ttggcattgc gcttcatcca caacgccagc
    37021 gctttcagcg agtctggagt gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc
    37081 cagcaaactt cactgacttc atcttcctgt agggcgaagg gaccgtgaga gacgcagctg
    37141 aacaacgcgc cccagacacg gcaattttta tcttcgaaat agaactgccc gtgctcggca
    37201 aaggggacac cggcaatgcc caactcttct tccgcttcgc gacgcgcgga ttccagcagt
    37261 tgctcatcgg cctgaaccac accgcctgcg gtcgcatcta acatgccggg taaaaagtct
    37321 tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc catcatgcac gacgatgtaa
    37381 gttgcacgat gacgcagaca ctgtgcccgc atttgttccc ggctggcttg tgcaatgact
    37441 tcgttctctt cattgacaat atccacccat tcagtacttg ccaaacgacg ctgttccatt
    37501 atcaggaaac cttctttttc tggcgctctt acggcgcatt ttgagttgtg ggtaaattac
    37561 ggattaatcg cgacctgcgc aatgatactt tgatcattga gtgcgattac actaagtacg
    37621 tcattatcca gcatgccata actcgccgga ttaccgcctt tcggaatact caccgagccg
    37681 gggttgaagt ggaaaatctc cccccgctgt tctgccactg gcagatgggt atgaccgtac
    37741 accagcacat cattctggtt taaagcaggt agattttccg ggccaaaaag atgaccatgc
    37801 gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc acggcgcggt tatcgggaaa
    37861 tgcagcagca tttgatccac ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt
    37921 gccacttcat taagccgttc agcgactttg gctggcgcgt aaccctccgg taaagcatta
    37981 cgcgggccgt gattcaacac gtcgccaagg atcaccagcc actgggcacc gctttgggca
    38041 aacaactcca gaacacgttc cgtcgccggt aacgacccat gaatgtccga tgcaaacatc
    38101 agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg
    38161 ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca
    38221 ctggaatgtc gcataatcca ccaggcgttc tggcacctca tcgccatgta gtaccaggcg
    38281 gttaatcatt agcgccagat cagtatcagc aatgcaccat tcaccaaata aattcggctg
    38341 acccagtact aacaaatgtt ctgccatcgc aaacagtttc tctgcactgg cttttccctc
    38401 ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg
    38461 gatgggcatc agatcgctgc gcagccaggc ctgaatctgt cgtgcacgcg cacgattttc
    38521 taaatcaagc ggataaatac gttcccaggt cggtggcgca aatcgatctt ccagatactc
    38581 cgcaatggca gaagattcac tcaactcaaa atcatcgatt tgtaataacg gcacacggcg
    38641 tgtctgaccg taaccttgcc acgtcggctg caaatgttca ccgctgtcga ggtcgatggt
    38701 cttgatatga aacgacaggc ctttttcctg caacgccacc caggcggata acacataagg
    38761 ggagaaaaag tgggcatctg accaaagcgt gatagcgggt ttactcatta tgtcctcggc
    38821 aataaagggg gctggctatc aaactataga gcctttttgt cactgtcacg tgacgaatac
    38881 tcacgtaatt cgccgcgctt tctatacttg tggtgttcag atgattaaac aaccggagct
    38941 gcaatgatcg atctctattt cgccccgaca cccaatggtc acaaaattac gctgtttctc
    39001 gaagaagcag agcttgatta tcgcttgatt aaggtagacc tggggaaagg cggtcagttt
    39061 cgtccggaat ttttgcgcat ttcgcccaac aacaaaattc cggcaattgt tgatcattct
    39121 cctgccgatg gcggcgaacc gttaagcctc tttgagtctg gtgccatttt gttgtatctg
    39181 gctgagaaaa caggactctt tttgagtcat gaaacgcgtg agcgcgccgc cacattacag
    39241 tggttattct ggcaggtagg cggactgggg ccgatgcttg ggcaaaatca tcattttaat
    39301 cacgcagccc cccaaaccat tccttacgct attgaacgtt atcaggttga aactcagcgt
    39361 ctttaccatg tactgaacaa gcggctggaa aactcgccct ggctgggagg cgagaactac
    39421 agcattgcgg atattgcctg ctggccgtgg gttaatgcct ggactcgcca gcgaattgac
    39481 ctcgcaatgt atccggcagt caagaactgg catgagcgga tccgttcgcg ccctgccacc
    39541 gggcaggcac tgctaaaagc acaactcggt gatgagcgtt cggatagtta acagaaacag
    39601 gttctcgtgt attatttcat cctaagtaaa acaacggaga acctgcaatg gcacaacctg
    39661 ccgctattat tcgtataaag aaccttcgtt tacgtacgtt tatcggaatt aaggaagaag
    39721 aaattaacaa ccgtcaggat attgttatca atgtgacgat ccactacccc gccgataaag
    39781 cgcgcactag cgaagatatc aacgatgcgc tgaattatcg caccgtaacg aaaaacatta
    39841 ttcagcatgt agagaataac cgtttctctt tgctggaaaa attaactcag gatgtgctcg
    39901 atatcgcacg tgaacatcac tgggtgacgt atgctgaagt ggagatcgat aaactgcacg
    39961 cgctgcgcta cgccgattcg gtatccatga ccttaagctg gcagcgttaa ccgccatatc
    40021 gggaggctgc atgaacatag tgatcaccgg agggacggga ttaattggtc gccatttgat
    40081 tccgcgtttg ctggagctgg gccatcaaat cacggtagtg acgcgtaacc cacagaaagc
    40141 cagttccgtt ctcggccctc gggtgacgct atggcaaggg ctagccgatc aaagcaacct
    40201 caacggcgtt gatgcggtaa tcaacctggc cggagaaccg attgctgata aacgctggac
    40261 tcacgagcaa aaagagcgtc tctgccaaag ccgctggaat atcacgcaaa aactggtcga
    40321 tttgattaat gccagcgaca cgccgccgtc ggtactcatt tccggctcgg caacgggcta
    40381 ttatggcgac ttaggtgaag tggtggttac cgaagaggaa ccgccgcata acgaatttac
    40441 ccataaactc tgcgcccgct gggaagaaat tgcctgccgg gcgcaaagtg acaaaacgcg
    40501 agtgtgcctg ctgcgtaccg gtgtagtgct ggcaccggat ggcggtattc tcggtaaaat
    40561 gctgccaccg tttcgtcttg gcctgggcgg gccgattggt tccggtcggc agtatcttgc
    40621 ctggattcat atcgatgata tggtcaacgg cattctctgg ctgctggata acgagctgcg
    40681 cgggccattt aatatggttt cgccctaccc ggtacgcaat gaacaatttg cccatgcgct
    40741 cggtcatgcg ctgcatcgcc cggccatttt gcgcgtcccc gcgaccgcca ttcgactgtt
    40801 aatgggcgaa tcttcagtac tggtattagg tggacaacgc gcgctgccta aacggctgga
    40861 agaagcgggt tttgcgtttc gctggtacga tttagaagag gcgctggcgg atgtcgttcg
    40921 ctgatgtggt ttacagcaaa catccgccag ttaactcccg gtgttacagg attagtggct
    40981 ttgcgcgata agatcgtctg gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa
    41041 acctctatcc agcatctcct gagcaatacg cagggcttct tcgtgtttgc cctgcattgc
    41101 gccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttctt gtggtgcgcg
    41161 ttccgctatc tcaccaataa atgcacgaaa acgctgggca tcccctgttt gtaatacgta
    41221 attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactagca gcgaaacaat
    41281 ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag
    41341 cgccattttg cggtgttgca taatctcgtc atccggcacc acggtaatat ccaccaacgg
    41401 aaacgctgat gaatatattt tgcgggctat agcaggctcg gcaaattcat caagccagca
    41461 gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga tcaccaatgg
    41521 aagctcttta tagcccgcat caagatggtt ttgcattgcc gcaatggaat aacgcatcat
    41581 gcgaaaagcc attaattctt ccggcttact ttggtgctct atcactacat aaatataacc
    41641 cactccctcc tgcgttttca cagaccacaa gaggtcggaa taatattgcc gcaggtcgtc
    41701 atcaataaaa ctgtttggtt ccagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg
    41761 cgcgggaaga tgaatatcaa taaaatcccg cgcggtgtct ggatggcgta aaaaagattt
    41821 aaataccgca tcatgcggcg tggaagttgt cgatattgtc atggcgttcc gtcacccctc
    41881 aaatttaaga tgacgcgaca ataaccacga ttaacaccca atgcagcagg cagtttttat
    41941 ctttggacag cgaattcaga agtattttta gcggactgca acttttacat ttcagcggaa
    42001 gctgactcgc aaattcccca tgaaaatact ccggcagcgt tagccaccgg agaggaaatt
    42061 atttcagcga tcccttaagg aactgttgca gacgagggct ttgcgggttg ccaaataact
    42121 gctccggcgc gccctcttct tctattttcc cctgatggag gaaaatgaca tgagtagaaa
    42181 catggcgagc aaagcccatt tcgtgagtca ctaccaccat ggttttcccc tcttctgcca
    42241 gttgctgcat aatacgcaac acttcgccta ccagttcagg atcgagcgcc gaggtaggtt
    42301 catcaaacag taaaacttcc ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt
    42361 gctgaccgcc ggaaagatgc accggatatt tcccctgcgc acgttcgtct atcccgactt
    42421 ttgccagata cttgaccgcc cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa
    42481 tcggcgcttc catgacgttt tccagcaccg tcatatggct ccagagattg aagtgctgga
    42541 ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt tttatcggcg actttgagtt
    42601 gaccgtcttt gtcgcgcacc agattgatcg tctggccatt gaccacgatc gacccttcac
    42661 tcggtttttc gaggaagtta atgcagcgca gaaaggtact tttccccgat cccgacgatc
    42721 cgatgatgct tattacatct ccggcattcg cttgcagtga gacccctttc agcacttcat
    42781 gttcgccgta gcgtttgtgc aaatcgataa cgtttaattt attctcggac atcgtattct
    42841 cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt tttccgctct gcgaaagagg
    42901 ctgatcagga cataagagat gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa
    42961 ggttgatacg tggcggcgtt aatatcgcgg gctattttca gcagatccgg caccgtggcc
    43021 gtaaacgcca gcgcagtaga gtgcagcatc aggatcactt cgttgctgta tgccggtaac
    43081 gcaatacgca gcgcagaagg caaaataatg caacgataca ttttaaaagt cgagaagcca
    43141 taggctctgg cggcttcaat ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc
    43201 tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca cggtacagtt caggccactg
    43261 cggaagaaag cgttaaggaa ttcggttccc ttaacaatct caagcgtgta catgccggaa
    43321 tagaacacca gcaactgaac atacagcggc gtaccgcgaa aaatataggt aaataaccag
    43381 attggaaact ggatgtattt attactggag acacgaccaa tcgccagaaa cagcgccagg
    43441 actccgccta tcactaccga caaaataagc agccacagag tgatcgccac accagtaaag
    43501 cgataaccgt cggtccacag cagcggtttc cagtattcat gtaagatttc gatcacaggt
    43561 cagccctctt cacacccacg gagtagcggc gctcaaggaa cagcagcaca ccattggaaa
    43621 cagtggtgaa aaccaggtaa atcacgccac agacgatggc gaaatagaac ggttcccagg
    43681 tacttttgcc tgccagttgg gtggctttga ccacatcttc caggccgagt aacgaaacca
    43741 gtgcggtaga tttgaggatc acctgccagt tgttgccaat gcctggcagg gcgtaacgca
    43801 tcatcgccgg aaacatgatc cgccgaaaca cttgcccacg agtaaaaccg aacgccgtcg
    43861 ccgcctctat atgccctttc ggcactgcca taaacgcgcc acggaacgtt tcggtaaaat
    43921 aagcaccgta gataaaaccg agagtaataa taccagcgac catcggatcg atatcaatct
    43981 gcccgacgcc catcgcctcc gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca
    44041 gcagcatcaa caccaagtcc ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca
    44101 gccccgaaag ccgattttgc gagagcttac cgccagcgcc aattaaaccg atgattacag
    44161 cgagcactac agaactgata gccagctcca gcgtgacgag cgcaccctgt aaaataacac
    44221 ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt
    44281 ctccctccgg ttacccgaag ggagcgggct ttctttcacc gatggcgatt aaccgccata
    44341 aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc
    44401 ggcaaaggct ttgttcagtg cctcgcgcag ttcgttatct tctttacgca ggcccatgcc
    44461 ggtccctacg ccaaacagtt tttcatcttt aacagacggg ccaccgaatt tgtaatcttt
    44521 accgacgggt tgtttgagga aaccttcact ggcagcgacc tcatcctgga acgcggcatc
    44581 aatacgtccg gcagtcaggt cagaataaat gttgtcctgc ccctgatacg agacgatttc
    44641 aatgcctttt ggtgcccaat gttcattacc gaacgtctcc tgggtggtgc cctgcaatac
    44701 gcctacccgt ttgcctttca gcgactcgac tgtcggctga atgtcagaat ttttcgccac
    44761 caccaaacga gaatcggcag cgtacagttt gtcggtgaag gctatttctt gctgacgttt
    44821 ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc ttcgctttta aggacgggat
    44881 taacgcatcc agcggatttt cgacaaacgt acattgcgta ttgatgcgtt tgcataattc
    44941 ctttgccaga tcgatatcga agccaaccag ttcgccttgt gaattttttg attcaaatgg
    45001 cgcataggtc gggtcggtac cgatgcggat gttttgcgga atcgcagcaa acgccgcagt
    45061 tgcgctggag aaggccagaa ccagagagag cgatagcacc agttttttca taatgtcctc
    45121 aactgacagt ctttttatag ggaatattta caggtttgtt acacttatcg tgccataaaa
    45181 ttgaccatca aggcaaaata tcccgccccg ttagacgtat tatttttcat taaaaatgct
    45241 taagtatgca tttcaatgca ccacgatagt gcaacaaacc tggcgtgcac catgatgatc
    45301 attccatcag gtacagcttc ccagcgacgt atcagtcacc gtagacatta aagtcgaaat
    45361 actttttcgc catcttgtcg taggtgccgt cctgacgcag ctcgccaagc gccttattga
    45421 aggcagccgt cagttcagca tcatctttac gtagccctac accggtgcca tcaccgaagt
    45481 attttttgtc ttttactgat gagccagcaa aggcgaaatc tttaccagca ggttgcttga
    45541 ggaatccttc gctggcagca acttcatctt gtaacgcagc atccagacgt cctgcagcca
    45601 gatcggaata gaccaaatcc tggttggcat aggccaccac atccacgcct ttgctacgcc
    45661 aggtctcgtt agcgtaagct tcctgggttg atccctgcag cacaccaaca tgtttacctt
    45721 tcagtgaatc cagcgttggc tgaatcggtg aacctttggc agcaatcaaa cgagaatctg
    45781 cggcgtacag cttgtcggag aaggcaatct cctgctgacg tttatcggta atggaaagcg
    45841 acgaaataat ggcgtcgatt tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac
    45901 tggcaaccca ggtacatttc acctgcatcc gtttgcacat ctcgttaccg agatcgatat
    45961 caaagccaac aaaatcacct ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg
    46021 ttccgatacg taccgtctcc ggtagcgccg catagctgga agccgctgcg gagagaccga
    46081 ctaacaaaga cagagcgaga atcgacttct tcatacataa ccctcaagtg aaaatggctt
    46141 ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt catgcaggtc ttatgccatc
    46201 ttgacgacaa cagtaacatt caacgttaaa tatgttaata agacgttgca ttattgtcct
    46261 gaagttgaag atagcaggta tagcggttga atcgcagcgt ttcgattgaa tggcagaaac
    46321 aaaatgtcga ggatttgatc gcggttgcaa aattgccctg aaacagggca acagcggagt
    46381 tatgcgccct gccagcgggc aaagagatct tcaggaaggg ttatcgcaaa ctggtcaaga
    46441 acacgattaa ccgtctgatt tatcacatca tcaagggatt gcgggcgatg ataaaacgcc
    46501 ggaacgggag gcataatcac cgcaccgatt tctgccgcct gagtcattaa acgcagatgg
    46561 cctaagtgca atggtgtttc acgcacgcag agcaccaacg ggcgacgctc tttcagcacc
    46621 acatctgccg cacgggtcag taagccatca gtatagctat ggacaatgcc ggaaagggtt
    46681 ttgattgaac agggtaaaat caccatccct agcgtctgga aagaaccgga agagatgctg
    46741 gcggcaatat cgcgcgcatc gtgcgtgaca tcggctaatg cctgcacttc gcgcagagaa
    46801 aaatccgttt cgagggataa ggtctggcgc gctgcctggc tcatcaccag atgcgtttcg
    46861 atatctgtga catcgcgcag aacctgtaat aagcgcacgc cataaatcgc gccgctggcg
    46921 ccgctgatgc ctacaatgag tcgtttcata aatagttgcc ctgtcagact tgcaggcaga
    46981 ctttgcagga tttcggcggg agttgcaagt cagggtgcca gaccggcacc ctcagcgaag
    47041 gcatcatcct tcgttatgca tttcgagatt ttccacttcg ttctgacgtt gcactgcttt
    47101 ggcgtcatca ttacgtaacg tatcgaggaa atcgaggtag ccctgatcaa catctttggt
    47161 gacgtagacg ccgttgaaca ccgagcattc aaactgctgg atatccggat tttcagcgcg
    47221 aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg
    47281 gcgaatttca tcaacttcgc gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat
    47341 accataaacg ttcgggaagc gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc
    47401 tccggcttcg cgtgccatct cgataatctg ctcagaagtg gtgccacgga cgatggagtc
    47461 gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg
    47521 cacggactta cgacgcagct gctggcccgg catgataaag gtgcggccaa catagcggtt
    47581 tttaacgaag ccctggcggt acggtttgcc cagaatacga gcaatttcca gcgcgatatc
    47641 acacgaggtt tctgggatcg ggatcaccac gtcgatatcc agatcttccc attcgcgggc
    47701 aattttctcg cccagtttcg tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc
    47761 gatgaacgag tccgggcgag caaagtatac atactcaaac aggcacggat tgctgaccgg
    47821 attgtcagca cattgacggg taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc
    47881 cggcgcgacg tcacgcagga aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc
    47941 gaccatatat tctgtacggt tctcgtcaat atcacgtttt cccagtacca gcggacgaat
    48001 cccgtttgga tcgcggaaag caaccatacc gtggccgata atcatcgcca cacaggcata
    48061 tgcgccgcgg attaagcggt ttgtggcagc aatggcagcg aaaatattgt cggcttccag
    48121 cgggtagtgg cggaagttgt ccagctcgct agcgaagata ttaagcagaa tttccgagtc
    48181 ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt tttttacgca actcgtgagc
    48241 gttggtcaga ttgccgttgt gggcaagcgt aatgccatac ggagagttaa cgtaaaacgg
    48301 ctgcgcttca gaggcgctgg agctgccagc cgtagggtaa cgcacatgac caatgcccat
    48361 attgccctgc aaacgctgca tatggcgagc ttcaaataca tcgctcacca gcccgttcgc
    48421 tttacgcaaa cggaagcagt tattggcatc tatggtgatg atgccggcgg catcctgacc
    48481 gcgatgctga agcaccgtta aggcatcata aatcgactgg ttaaccggca taacaccggc
    48541 gataccgaca ataccgcaca tacgtctttt tcctcgttaa gccacatctc agagcactta
    48601 cgctctgggc aagaaacttg acgagctttg cagataatca aaaaaccatc tgatgataaa
    48661 actgaactgc gggatcagct gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa
    48721 ggagtcgaga aagaagagaa tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca
    48781 gacgcccagc acccgatcgg tgcctgacaa ccccgttttc tccaccaact ggcctatcac
    48841 gaagttcacg atagcaccaa cgatcagggt agcgataaac agtaccgcga tggcaattcc
    48901 atttcgaacc agttcgtctt caaagcccgt aaaccagact gacaggtaag tgtagtaatg
    48961 actggcaaca aagaaagcac aaccccatgt caccagcgat aacgcttcac gaacaaagcc
    49021 gcggatcagg ctaaccagag aggaaaaagc aatcaccgcg attatggcgt aatcaatcca
    49081 gaccatatgt gtcccacgat tttacgccct gtcatcctgt tcggggcgca ttctaacaga
    49141 aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc aataaaaatg gcgctgaaaa
    49201 aatattcaac gccatcgact ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc
    49261 ggcgtaaacg ccttatccga cctacggttc taacactgcc caggcctgat aagacgcgcc
    49321 agcgtcgcat caggcaagac tgtattaatt cggcgtatag cccattacca cgccacttaa
    49381 gccagaaagt tgcttcaact cacccagcga acctttcagc ttatctttcg aggcatccgg
    49441 cccaaccaga atacgggtaa ttttaccctg cactggcgtg gatggcgacg tataaacccg
    49501 ataaccggca ccgcgcagct taccgacaat ctcattcact ttatcggcat ttttcagcgc
    49561 acccagttgc acaacatagg ctttacccgt tggcgctgct ttttcttcca cgaccggctt
    49621 cggttctggc ttcggcgcag gtggtgcttc aaccttcggt ttaggcggct ccaccggttt
    49681 cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac tcggtgttat tggctgcaat
    49741 agtggtcgga tcgagcgacg gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc
    49801 ttccggcggc tgcgtcggca acgcctgggt ggcggctggc atcatatcag gctcatcgcg
    49861 atcgcccgct ttcggcacca gcgggatagc cgcgaactca tcctgataat gttttttctg
    49921 cccgtccagc agccctggaa gtacaatcac ccccagcgcc accagcacga tcgtgcccac
    49981 taaccgattc tgaaacttac ttgccaccgc ttctcctcgc gtcaatcact tccatgacat
    50041 gtgcgacagt gtggaaagaa ccacacacca gcacggtgtc ttccgcttta gcgtccgcca
    50101 ttgcggcatc ccatgcctgc gcaacgctat caaatgattt gccgttaccc aaatgctcaa
    50161 gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa
    50221 ccacgctttt caaccaggcc agagttccgg caatatcttt atcatgtagc ataccgataa
    50281 ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg cccggtgaga tattccgccg
    50341 catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac
    50401 gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc attttcactg acttccagcc
    50461 cgctggcacg cagtgccgcc agcgccgttg cggcattcgg ttgcgggaca agcggcaacg
    50521 gcagattttc cagcgtgccg tgagcatcgc taaacgccca gtcatgatcg gtgacggaat
    50581 agttccactc aacgccccga cgttgtaaca gtgcaccttt ttcctgtgcc acatcagcaa
    50641 tggtagaagg catttccggc tcaccgacaa ttgccggttt ttcgctgcgg aagatgcctg
    50701 ctttctcgcg accaatactt tcgcgatctg gacccagcca gtcggtatga tccagcgcaa
    50761 tactggttac taccgcgaca tcggcatcga caatattggt tgcgtccaga cgaccgccca
    50821 gccctacttc cagaatcacc acgtcaagtt gtgcctgctt gaacagccac aacgccgaca
    50881 gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg tgccgattca atctccgcaa
    50941 aagaggcggt gtgggccgat tccggcaatt cctggccctg cacacgtacg cgctcggtat
    51001 aacgcaccag atgcggtgaa ctgtagacgc ccactttgta ccctgccgcc atcagaatcg
    51061 actccagcgt acggcaggtg gtgcctttgc cattcgtacc cgcaacggta aacacaaatg
    51121 gcgctggttt cagaacgcca agacgcgccg cgacctggct cacgcgctca aggccgagat
    51181 cgatagtttt actgtgcagg ttttccagat aagaaagcca cgaagccaga ggcgacgcgg
    51241 cttgaggagt gcgtttgata atcatggtat ccgctgattc gttacggtga gaatagcaaa
    51301 agggcagagc cagtggccct gcccttatca gttatcaggc ctcaggttcc tgatccggta
    51361 ccgggggtac cactacgcct tcacgcggcg cttcaggatt cggcgctggc agattcatca
    51421 acttcgccag aatgctcgcc agtttcaggc gcatttccgg acgacggacg atcatgtcga
    51481 tggcgccttt ctcgatcagg aattcactgc gctggaatcc tggcggcagt ttttcgcgaa
    51541 cggtctgttc gataacacgc ggaccggcaa agccaattaa cgctttcggt tcagcgatgt
    51601 tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc catcgtcggg tcggtcagca
    51661 cggagatgta cggcaagccg cgctcctgca tttttgccag tgccgcagag gttttcgcca
    51721 tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc accagaggcg gagaagcaga
    51781 tcagcgggca gttatcttcc agcgcctgct caacggcacg cacgaaacgt gcacccacaa
    51841 cagaccccat tgaaccgccc ataaaggcga actcgaatgc cgcagcgaca accggcattc
    51901 catacagagt gcctttcatc accaccagcg catctttttc gccagtttct ttctgcgcag
    51961 atgccagacg gtctttatac ttcttggagt cacgaaactt cagcacatct ttcggctcaa
    52021 gctcgctacc cagctccaca aggcttcctt catctaacag gctatgcagg cgattacgcg
    52081 ctgtcatacg catgtgatgg tcacacttcg gacagacctc aagattacgt tccagctcag
    52141 cgcggtataa aacctgaccg cagctatcac acttagtcca caccccttca ggaatgctcg
    52201 ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat ccagctcatt agggaccttt
    52261 ctgtctgaac ctggttcgat gccagtttta tctttgggga cgcataatgc catttttgcc
    52321 cccaacagac catgaatgtt gcacattaaa acataacagc ccgaaacttt ggataaaaaa
    52381 gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg
    52441 aataaattcg aaaaccccag gcaatattga aacaaaaata atgccaacaa tcatcagctt
    52501 aaggttgctc tgaatgaagg ggagcgtgcc gaagaaataa cccgcgtagg taaaaatcag
    52561 tacccacaac agtgcgccga tcacgttata agcggcgaaa tgacggtacg acatgtggcc
    52621 cattcccgca acaaacggcg cgaacgttct gacgatcggt acaaaacggg cgagaataat
    52681 cgttttgccg ccatgtctct cgtaaaactg atgagtctta tcaagataac tgcgacgaaa
    52741 gattttcgaa tccgggttac tgaacagttt gtcaccaaat aaccgtccaa tggtatagtt
    52801 cactgcatca ccgacaatag cggcaatcaa cataagcacc accatcatat ggacattgag
    52861 atcgttagtt tccagcgacg ccaatgcacc agcaacaaac agcagcgaat caccaggcag
    52921 gaatggcgtt accaccagcc ccgtttcgca gaacagaatc aaaaacagaa tggcataaac
    52981 ccagacgccg tactccgcga ccagctctgc cagatgcacg tcaatgtgca ggataaaatc
    53041 gatgaggaaa taaatcaggt ccatatttgc ctatgccttg tactcgtcat ctttcaggct
    53101 gtaactacgt tggctacagc ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa
    53161 atagcgggcc cattggcggt tttggaagat cataccggtc agggtaatcc accgcgacca
    53221 gatacagccc ttccgctttt gccgttgctg ccgccagcgt tctgtccttt gccgccagca
    53281 actctgctat ccagctctcc ggctggttgt gggcaccgac ttccatcagg ctgccgacaa
    53341 tattcctgac catatgatgt acaaaggcat tcgctttgat atctaccacc acataaggac
    53401 cgtgacgcgt gacattaata tgcataacgt tacgccacgg ggttcgggac tggcactgca
    53461 ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc
    53521 gttcagcgtc cagcggttcg taaaaatggg ttaccccttt actcagtacc gccgggcgca
    53581 gccgatgatt gtagatgatg tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa
    53641 aatcatcagg tacagcttta acccaacgca cggcgatgtc accaggtaaa ttcgcattta
    53701 cgcccaaggt ccacgccgcg tctttgcgct gcgcggttgt ttcgaaatgc accacttgcc
    53761 cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg
    53821 ccacctggga gagcgccttt tccagcttct cctgcacact gcggacttca ttctgccgtt
    53881 gccagccgta atacttactg ccgtcgtact caatgcccag cgcaatttta taaactggca
    53941 gttgttgctg gtcggacatt agtacagata ctcctgcacc agtttctcgg cgatttttac
    54001 tgccatcagc gcgccgccaa agcgaacgtt atcggccacc gaccagaact ggacttgctc
    54061 cggcataccg tagtcattac gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc
    54121 ccctacctga gtcgggaatt cgttctcttc agagagcaca atatcttcgc cctgagcaaa
    54181 cgcatcacgc gcttcttctg ccgccagcgg acgcagtgct tcaaagttga ccatctgggc
    54241 atgaccgtag aataccggtg cctggacgac gcttgccgaa atcatcagcc cttcgtcctg
    54301 caggattttg cgtacttcgt caacgatacg acgttcttca cgcacgctac cttcgctatc
    54361 cggcaacaac ggcagcatat tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat
    54421 cggaatgccg ttgagcaatt tcgcactctg ccccgctaac gcatcgaccg cttttttgcc
    54481 ctgggcggag gctgaaatca ggctggtaac actgatacgc gacaaaccgc cctgatcgat
    54541 taacggtttc agtgccgcca gcagctggct ggtcagactg tctggtacgg cgatgacatt
    54601 ccggttgcgg taatcggtca gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc
    54661 gggttcgaga gcaaacagtc cgctgctgtc gatcaccagg caacctgagt tggtcgcttc
    54721 ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt
    54781 ccagtcgaat tcagcggcat cctgcacggt gattgtctta ccaccaaagc gcagttgttc
    54841 gcctgcgctt tcgttacgtg ccagtgcata aatttcccca accgggaact gacgttcagc
    54901 cagcgtttca agcagggctt cgcccacagc gccagttgcg cccaggacgg caatgttcca
    54961 gccttcagac atggtggttt actccagaaa tagcaaagct ccctgccaat gttacagcag
    55021 agagcatgaa gaagagatta acgtgccgga tgatgaacgg cgttaaaacc cagtttacac
    55081 agcaatgatg ccgcactggc gtcatcacaa attacataca gagatgacca ttcacggcgc
    55141 tcaagatagt ttttgcgcag tttatcgaac tcacccggta tcccggcgac tttacgcagc
    55201 ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca gccttttcag cgtcggttga
    55261 tcgagcgggc catgcagcgt aatgcgacca aactctggcg cgggcagtaa tgtatccagc
    55321 gcaacgtgct gttcatgccc aataaacttg ctataggctt caaacacttg cgtagtaccg
    55381 cgtgctttac cttccagggt ataacctgcg atatgcggcg tgccgatatc cacttttgtc
    55441 agcaactcca cgttaagttc cggttcgcct tcccagacat ccagcactac gcttaacttc
    55501 tggccttcat tcaggcaggt cagcaacgca gtattatcga cgactgcgcc acggcaggcg
    55561 ttaatcagaa tcgctcccgg cttcaggcta cggatcagtt tttcatccgc cagatgcagc
    55621 gttttgtacg gcccatcttt gaagagtggc gtatggaaag tcagaatatc cgcatgctga
    55681 actaattcat ccagcgagcg gaaatcaccc tcatccccac ggtcagcgcg aggcggatcg
    55741 caaagcaatg ttttaatccc taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta
    55801 ccaacgccca cgatccccac cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc
    55861 atcagtagtg aggagaaaac atattccacc accgcaatcg cattacagcc aggtgcagcg
    55921 gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga catggtcggt ccccgcagtg
    55981 gcagtgccaa caaatttaat gggttttcct gccagcaaag attcattcac tttcgtgacc
    56041 gaacgcacca tcagcgcatc cgcgtctgcc agttgagcaa cggggattgg acgcccggga
    56101 accgcggtca cctcacccaa acggctaaat aagtcgcggg cataaggcat attttcatca
    56161 acaaggattt ttaacccttt ttcagtctct gacaatctaa ggctccttta tcaacaaacg
    56221 cccatttgca aactgctgtt taatctgaaa aaacattaat aatcatatag ataacaacat
    56281 aaccatttag acgggcaata ctgaaaaatt gttattcagt atcgcccttt aagccgtttc
    56341 aggctatata aaattaatcc cccgtggaca ctgcgtggac acttacgcca cctttcagcg
    56401 gattaagggc caccgcatcc tgcaggtaat ccggtgcaaa atgcgcatat gccattgttt
    56461 gctgaatggt tgcgtgacca agaatcttct gaagcgcaat aatgtttcct ccgttcatca
    56521 caaaatggct ggcgaacgta tgccgcagca catgtgcagc ctggcctttt ggtaaatcgg
    56581 gcttaactct tttcagcgcc aggcagaatt cccggtactt cacctcaaac aagccgcctg
    56641 tttctctggt tttgatcgcc tcacaaactg cctgcgaaat tggcactgtt ctcttccggc
    56701 catttttggt ttcaagaaac gttacacggt tatgaactat ctgttcacca cgaagcttac
    56761 aagcttcact ccagcgcgcc cctgtgctta aacacaaaag cgcaacacgc cagtagtcgc
    56821 cctccagtgt atcgagcaat agcgccactt ccttctgtga caggaacgcc atttctcgtg
    56881 gagatacata aagaatagaa attcccctta ctggatgttc tgcatcccaa agacctattt
    56941 ttttcagcac ggtaaacatt ccggataacc gattcatgta cctgttagca gatgacggtt
    57001 tcaatccttc agctatcttt tgagaacgcc acgcgataat tttcagctta tcaagatcca
    57061 cagcctgcat atcagcgcca agctcattga ttatgttgcg cagttgtttt cggtcttctt
    57121 ccgccttacg cctgtgctgg ccgtgataca tccaccacaa ctcaagcaaa tcatttagcg
    57181 ttcgacgatc acggtagccc tgtatatatt cccgcttttc agcgttcgcc atgatgtagc
    57241 gttcagtggc caccgctacc gattttttgt caaatacctt acgcacgcgc tttcccttgc
    57301 gtccgttcgg cctgatgtcc agcaaataac gaccatcttc gagcttctta atagacatta
    57361 cgaagccctc caatgaaccg ctctacaatt tctccagctt ctttccagca ataatcagac
    57421 cagacaaaaa gcaggtctaa ccagttttct ggcctcagcg ggataatttt tggattgttt
    57481 cggttgaaac gcgatctccc tccgaattgc aggaaccatc aagagagaga gccggagaaa
    57541 tttgcccgac ctccggcatc gtttcatctg ttgataacca ataagcatac ttcttaaatt
    57601 tagggtgttt cgtaacctta agtaaggcac cctctgttac ttgcttcccc ctcacctcat
    57661 agttagttac cgtcccataa ggcagcccaa cacaatccgc aaactcctgg cgggtcatac
    57721 cttctgcttc acgaatcagg cgaaattttt cacccatgct tgacaaagat gccatatcgg
    57781 gcatatcctc catcgaaaca tgccacatcg ggcacaaaca taaaaacact caaataagcc
    57841 gatataagcc attttgagcc attcgaacga attagggaga ttaccacaat gagcgaatca
    57901 gagcttgggg ggttcattca ggtagcacca tatccacttg aagcggtgcc atatcaacta
    57961 ttcgccaaga tgatcggccg caaggaatcc acagtcagaa ccatgattga cgcagcaaag
    58021 ctaccgacaa ttgactttgt gaaaccaggt tcagtaaaga cgcgtgcatc agaaaactgg
    58081 gtatatctgc cagcatttaa cgaaggcatg cgcaaagcgt tttttgagca accgaaagaa
    58141 cgccgcgacg catggttgtt gtggctggga ctttagtcat aaatgaccag ccatatcatc
    58201 agcgccattc tgacccttgt ttttattgag ataggcgtaa tagcgatcta tttgtttcgc
    58261 aagttaacgg gacatgaaga acgctttatt gaactcagca ttgaatatat cgccgcttat
    58321 accaaaggac ttttcccggc agctattggg gcgatgttta tagcgtttgt tatctggttt
    58381 atcgggtgag aaacctgaca acccacaact actgcaatga gggtaattat gtgtggcatg
    58441 gacaaattgg accttatgtt aattgtaata ctatcaatta acttcggtta cctcttaagc
    58501 ggcgcaattc tcatgtgcgg aggcaaacga aaatgaacca gcagtccgca aaacgtgaaa
    58561 acaatgcgat gcgatttaat cgcaaatatt ttgagttcgg cctctatgtc ggaataatta
    58621 aatctgtaca gagtctttaa catgaaacag caacgtaatt cacgctttcg caatggtgca
    58681 gaacgccacg ctaaccgttt tgctaccagt gcatcacgca gcaacatccg ctacagcctg
    58741 agtgatacac acgcaacgcc ggatggccat acagtaaaac aaattggcga acacgcctgg
    58801 ttgattgaga aagcaggaat cgtagtccac aaatgcccac gcaatccgtt taccggaaac
    58861 cgcatttttg cattgagctg cgg
//
LOCUS       DAHBHC010000021        58167 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHBHC010000021 DAHBHC010000000
VERSION     DAHBHC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.021"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..737
                     /locus_tag="M2A35_001683"
     CDS             <1..737
                     /locus_tag="M2A35_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HCB7197997.1"
                     /translation="WCAEYDEWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRY
                     YDPLQGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEIN
                     IPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNS
                     FASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLG
                     NWITYGPSGHPISNMQGTPEKPSDIR"
     gene            749..1210
                     /locus_tag="M2A35_001684"
     CDS             749..1210
                     /locus_tag="M2A35_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCB7197998.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            1917..2354
                     /locus_tag="M2A35_001685"
     CDS             1917..2354
                     /locus_tag="M2A35_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7197999.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2814..3950)
                     /locus_tag="M2A35_001686"
     CDS             complement(2814..3950)
                     /locus_tag="M2A35_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCB7198000.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(3953..4315)
                     /locus_tag="M2A35_001687"
     CDS             complement(3953..4315)
                     /locus_tag="M2A35_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCB7198001.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            4852..6753
                     /gene="mtlA"
                     /locus_tag="M2A35_001688"
     CDS             4852..6753
                     /gene="mtlA"
                     /locus_tag="M2A35_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCB7198002.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            6848..7996
                     /gene="mtlD"
                     /locus_tag="M2A35_001689"
     CDS             6848..7996
                     /gene="mtlD"
                     /locus_tag="M2A35_001689"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCB7198003.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            7996..8583
                     /gene="mtlR"
                     /locus_tag="M2A35_001690"
     CDS             7996..8583
                     /gene="mtlR"
                     /locus_tag="M2A35_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCB7198004.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(8595..8804)
                     /locus_tag="M2A35_001691"
     CDS             complement(8595..8804)
                     /locus_tag="M2A35_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198005.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            9089..9451
                     /locus_tag="M2A35_001692"
     CDS             9089..9451
                     /locus_tag="M2A35_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCB7198006.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            9995..10678
                     /locus_tag="M2A35_001693"
     CDS             9995..10678
                     /locus_tag="M2A35_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCB7198007.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            10722..15572
                     /gene="ehaG"
                     /locus_tag="M2A35_001694"
     CDS             10722..15572
                     /gene="ehaG"
                     /locus_tag="M2A35_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCB7198008.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            15940..17595
                     /gene="lldP"
                     /locus_tag="M2A35_001695"
     CDS             15940..17595
                     /gene="lldP"
                     /locus_tag="M2A35_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCB7198009.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            17595..18371
                     /gene="lldR"
                     /locus_tag="M2A35_001696"
     CDS             17595..18371
                     /gene="lldR"
                     /locus_tag="M2A35_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCB7198010.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            18368..19558
                     /gene="lldD"
                     /locus_tag="M2A35_001697"
     CDS             18368..19558
                     /gene="lldD"
                     /locus_tag="M2A35_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCB7198011.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            19744..20217
                     /gene="trmL"
                     /locus_tag="M2A35_001698"
     CDS             19744..20217
                     /gene="trmL"
                     /locus_tag="M2A35_001698"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCB7198012.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(20270..21091)
                     /gene="cysE"
                     /locus_tag="M2A35_001699"
     CDS             complement(20270..21091)
                     /gene="cysE"
                     /locus_tag="M2A35_001699"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCB7198013.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(21171..22190)
                     /gene="gpsA"
                     /locus_tag="M2A35_001700"
     CDS             complement(21171..22190)
                     /gene="gpsA"
                     /locus_tag="M2A35_001700"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCB7198014.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(22190..22657)
                     /gene="secB"
                     /locus_tag="M2A35_001701"
     CDS             complement(22190..22657)
                     /gene="secB"
                     /locus_tag="M2A35_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCB7198015.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(22720..22971)
                     /gene="grxC"
                     /locus_tag="M2A35_001702"
     CDS             complement(22720..22971)
                     /gene="grxC"
                     /locus_tag="M2A35_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCB7198016.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(23113..23544)
                     /locus_tag="M2A35_001703"
     CDS             complement(23113..23544)
                     /locus_tag="M2A35_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCB7198017.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            23789..25333
                     /gene="gpmM"
                     /locus_tag="M2A35_001704"
                     /gene_synonym="pgmI"
     CDS             23789..25333
                     /gene="gpmM"
                     /locus_tag="M2A35_001704"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCB7198018.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            25343..26626
                     /gene="envC"
                     /locus_tag="M2A35_001705"
     CDS             25343..26626
                     /gene="envC"
                     /locus_tag="M2A35_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCB7198019.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            26630..27589
                     /locus_tag="M2A35_001706"
     CDS             26630..27589
                     /locus_tag="M2A35_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCB7198020.1"
                     /translation="MFPFRRNVLAFATLLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(27576..28613)
                     /gene="waaH"
                     /locus_tag="M2A35_001707"
     CDS             complement(27576..28613)
                     /gene="waaH"
                     /locus_tag="M2A35_001707"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCB7198021.1"
                     /translation="MYDEQPSKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIIND
                     GSTDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPT
                     MYETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSS
                     RRWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYL
                     HNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVC
                     HAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSH
                     HRITRSAFNLR"
     gene            complement(28848..29873)
                     /gene="tdh"
                     /locus_tag="M2A35_001708"
     CDS             complement(28848..29873)
                     /gene="tdh"
                     /locus_tag="M2A35_001708"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCB7198022.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(29883..31079)
                     /gene="kbl"
                     /locus_tag="M2A35_001709"
     CDS             complement(29883..31079)
                     /gene="kbl"
                     /locus_tag="M2A35_001709"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCB7198023.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(31354..32211)
                     /gene="yibB"
                     /locus_tag="M2A35_001710"
     CDS             complement(31354..32211)
                     /gene="yibB"
                     /locus_tag="M2A35_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCB7198024.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            32515..33447
                     /gene="rfaD"
                     /locus_tag="M2A35_001711"
     CDS             32515..33447
                     /gene="rfaD"
                     /locus_tag="M2A35_001711"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCB7198025.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            33457..34503
                     /gene="rfaF"
                     /locus_tag="M2A35_001712"
     CDS             33457..34503
                     /gene="rfaF"
                     /locus_tag="M2A35_001712"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCB7198026.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            34507..35499
                     /gene="rfaC"
                     /locus_tag="M2A35_001713"
     CDS             34507..35499
                     /gene="rfaC"
                     /locus_tag="M2A35_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCB7198027.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            35496..36704
                     /gene="rfaL"
                     /locus_tag="M2A35_001714"
     CDS             35496..36704
                     /gene="rfaL"
                     /locus_tag="M2A35_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCB7198028.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(36740..37882)
                     /locus_tag="M2A35_001715"
     CDS             complement(36740..37882)
                     /locus_tag="M2A35_001715"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCB7198029.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(37891..38904)
                     /locus_tag="M2A35_001716"
     CDS             complement(37891..38904)
                     /locus_tag="M2A35_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCB7198030.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(38929..39636)
                     /gene="rfaY"
                     /locus_tag="M2A35_001717"
     CDS             complement(38929..39636)
                     /gene="rfaY"
                     /locus_tag="M2A35_001717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCB7198031.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(39662..40669)
                     /gene="waaO"
                     /locus_tag="M2A35_001718"
                     /gene_synonym="rfaI"
     CDS             complement(39662..40669)
                     /gene="waaO"
                     /locus_tag="M2A35_001718"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCB7198032.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDV
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(40712..41509)
                     /gene="rfaP"
                     /locus_tag="M2A35_001719"
     CDS             complement(40712..41509)
                     /gene="rfaP"
                     /locus_tag="M2A35_001719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCB7198033.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(41502..42626)
                     /locus_tag="M2A35_001720"
     CDS             complement(41502..42626)
                     /locus_tag="M2A35_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCB7198034.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAVFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(42623..43681)
                     /gene="rfaQ"
                     /locus_tag="M2A35_001721"
     CDS             complement(42623..43681)
                     /gene="rfaQ"
                     /locus_tag="M2A35_001721"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCB7198035.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            44094..45371
                     /gene="waaA"
                     /locus_tag="M2A35_001722"
     CDS             44094..45371
                     /gene="waaA"
                     /locus_tag="M2A35_001722"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCB7198036.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            45379..45858
                     /gene="coaD"
                     /locus_tag="M2A35_001723"
     CDS             45379..45858
                     /gene="coaD"
                     /locus_tag="M2A35_001723"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCB7198037.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(45897..46706)
                     /gene="mutM"
                     /locus_tag="M2A35_001724"
     CDS             complement(45897..46706)
                     /gene="mutM"
                     /locus_tag="M2A35_001724"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCB7198038.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(46804..46971)
                     /gene="rpmG"
                     /locus_tag="M2A35_001725"
     CDS             complement(46804..46971)
                     /gene="rpmG"
                     /locus_tag="M2A35_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCB7198039.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(46992..47228)
                     /gene="rpmB"
                     /locus_tag="M2A35_001726"
     CDS             complement(46992..47228)
                     /gene="rpmB"
                     /locus_tag="M2A35_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCB7198040.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(47445..48113)
                     /gene="radC"
                     /locus_tag="M2A35_001727"
     CDS             complement(47445..48113)
                     /gene="radC"
                     /locus_tag="M2A35_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCB7198041.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            48285..49505
                     /gene="coaBC"
                     /locus_tag="M2A35_001728"
     CDS             48285..49505
                     /gene="coaBC"
                     /locus_tag="M2A35_001728"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCB7198042.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            49483..49941
                     /gene="dut"
                     /locus_tag="M2A35_001729"
     CDS             49483..49941
                     /gene="dut"
                     /locus_tag="M2A35_001729"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCB7198043.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            50048..50644
                     /gene="slmA"
                     /locus_tag="M2A35_001730"
     CDS             50048..50644
                     /gene="slmA"
                     /locus_tag="M2A35_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCB7198044.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(50681..51322)
                     /gene="pyrE"
                     /locus_tag="M2A35_001731"
     CDS             complement(50681..51322)
                     /gene="pyrE"
                     /locus_tag="M2A35_001731"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCB7198045.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(51388..52104)
                     /gene="rph"
                     /locus_tag="M2A35_001732"
     CDS             complement(51388..52104)
                     /gene="rph"
                     /locus_tag="M2A35_001732"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCB7198046.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            52231..53094
                     /locus_tag="M2A35_001733"
     CDS             52231..53094
                     /locus_tag="M2A35_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCB7198047.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            53283..54551
                     /locus_tag="M2A35_001734"
     CDS             53283..54551
                     /locus_tag="M2A35_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7198048.1"
                     /translation="MPKLTDMQIRAWIKAGDRFDGKADGNGLYICYPKNYTVPFWRFR
                     YKLAGKQRAMVIGSYSELSLSKARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAMRVSDLAAEYFERQILPRWKHPDILRRRIDKDINPCIGHMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVSDAGGKEVSRDRWLTRD
                     ELIRLFEAMRTAKGFSRQNELTFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGVYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPFDKARRQM"
     gene            54548..55198
                     /locus_tag="M2A35_001735"
     CDS             54548..55198
                     /locus_tag="M2A35_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198049.1"
                     /translation="MNNILRKSPIERLCSSVSITPHEMAVALAGLNPSMRISDVPEEN
                     FEYVDFVRTHLARAIKVYRGEKTSKDEPCHALDIFLASYPFIDTNTPEIIVQKISEAI
                     DDLRGTKGWEEKARNLGGLQLVNYIKETNRSGRGQHRKQDEENGTMKMMGLIVHLLIK
                     KSGSSSYIQNGKPNRTAIYRDIETLIKDEGISHKGIAKATFLEKISRALLAVHDLD"
     gene            55670..55888
                     /locus_tag="M2A35_001736"
     CDS             55670..55888
                     /locus_tag="M2A35_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCB7198050.1"
                     /translation="MTAHKPASSAIPTTGYIRRFRMPELLGVSLPTIDRWVKNGTLPR
                     PVKLTDNVTAFDAVEINNWLAERRNTSA"
     gene            55963..56661
                     /locus_tag="M2A35_001737"
     CDS             55963..56661
                     /locus_tag="M2A35_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7198051.1"
                     /translation="MKLRKTPVQGQGFVRPETSMSDILVRHKAESRIDSRLFAERIGI
                     KHKNLYSLIQKHKTGLRELGILPFQTERLKTRQNGEREHKFALLNSDQFDYMCRIVRG
                     RDDERMNRFKLDVTKAFAKHRATEPVRREYLPGYHESRDGLKALGAERHHYINLARAE
                     NRVTGLVTGERGTASEQQLGLLIVMQKIEQAAFEEAIQNGMTPTEAVREVARRMETFA
                     SLMTSEPYLGVNHA"
     gene            56654..57397
                     /locus_tag="M2A35_001738"
     CDS             56654..57397
                     /locus_tag="M2A35_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCB7198052.1"
                     /translation="MLKDFAAIIPVSPGIIGGHETAIVSARALHKALGVKRDFTTWIK
                     GRIDQYGFKNGVDFEFVENLTSPNPVSAKSRQRVEHDYLSTINMAKELAMVERTEQGR
                     AIRQYFIKCEEELHKVAPVRSAALRRELKARITVASYFKPMCAALEAYRAELGKNTLQ
                     HHYTTEANMLARIVLGGMNAKQWAQANGITGEPRDHMSTLQLEHLSYLEQSNITLIEL
                     GQDYHQRKAELIRLSQRWLARRMEVSSHA"
     gene            57390..57947
                     /locus_tag="M2A35_001739"
     CDS             57390..57947
                     /locus_tag="M2A35_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCB7198053.1"
                     /translation="MRKPVFYAGRTEISVIKFDDIQVRIVSINGEPWFVGADVCAALE
                     ISNVTDAVSVLDNDEVMTLALTEGHSGKRGGARSWNVVSESGFYKLIARSRKASTPGT
                     FAHRFSNWVFREVIPSIRKTGGYGVPFAMLNDFSKRQAAYQQEASKRGYRLQQCKEAK
                     DALEREERALWRKYQPDFLEGESNV"
BASE COUNT        15475 a        14169 c        13916 g        14607 t
ORIGIN      
        1 cgtggtgcgc agaatatgat gaatggggca acctgctgag tgatgagaac ccgcatcatc
       61 tgcagcagct cattcgcctg ccgggtcagc agtatgatga ggagtccggc ctgtattaca
      121 accgccaccg ctattatgac ccgctgcagg ggcggtatat cactcaggat ccgattgggc
      181 tgaagggggg atggaatttt tatcagtatc cgttgaatcc ggtcataaat gtagatccgc
      241 aaggtttggt tgatataaat ttataccccg aaagtgatct tatccattct gtagctgatg
      301 agattaatat cccaggcgtt ttcacaatcg gggggcatgg tacccccaca tctattgaat
      361 ccgcaacgcg cagtatcatg acagctaaag atctagcata tctaattaaa tttgatggga
      421 attataaaga tgggatgaca gtttggttat tttcttgtaa tacaggtaaa ggacaaaatt
      481 catttgctag ccaattagct aaagagttac atacaaatgt aataggacct gacacgctat
      541 ggacgtggtg ggggcgagga actaatggta agttaaaaat ggatacagtg ctaacagcac
      601 caacgaacct taattcaaat aaggatctaa tggctataac aacaaaagac cttggtaatt
      661 ggataacata tgggccatct gggcacccca tttctaatat gcaaggtacg ccagaaaaac
      721 ccagtgatat aagataggtt gtagatgtat gaaagcatgc ttgttactat ttttttattt
      781 ttctcttatt ggtcaattgc atggtgctga tgtgaaaata aaagaaaacg aaagtgtgat
      841 gggatctaca gcaatgacct atgatttaag tgaagaaaag ctgatgaaac tgaagtataa
      901 atcacaacat ggtgattcag aggcatcatt tcgactatat caatattact gctttactaa
      961 aaataatatt gataaacaat tgcgattctt ggaaagatca gcatctcagg ggaatgttac
     1021 agcgcagttt aattatgggg tctttttatc agatacaaat ccaacattat cagaatatta
     1081 taatttgaat agagctattt attggatgga attcgctgta aataacggta atattgatgc
     1141 aaagagcaaa cttcaagagc tcaaaaagct aaagcgaatg gatagaagga agaataagga
     1201 gaatccgtaa aagcaatagt tcatcagact gccgcagcag tagtatgatg atgaatcagg
     1261 gctgtactat aactattatc ggtaacatta accgcagcag gggcggtata acactcaggc
     1321 tccgattggg ctggaaggta ggtggaattt ttatcagtat ccgttgaacc cgatttctgg
     1381 aatcgatccc cttgggatta gccacctgtt tatacagtat aactttaggt atgctttcgt
     1441 gtgtttcaga tataccatgt gatgataatt tttatgatgt gcttagtatt ccagtcgctt
     1501 ctggtaataa tatgcaatgt aaaaataatc cccgttgtac acatttataa atagatggcc
     1561 aatacctcaa ggcctatgga gttagaatgt taatgacccg ggagcaagca atagaaaacc
     1621 tgacggcata cgattagtac cgtcagttaa tacaggaact tataatcgca atggattttc
     1681 aatacatagt tgtttaaacg cttttggtcc gtctttagga cctcgattct gttccgaggg
     1741 ttgtataact ggtttatcta atgacatgca aaaattaaat gagttaatat tttcagagcc
     1801 agatagcaca ttaacagtta cggactaaaa aatatggcaa ggttattaac aattgtattt
     1861 ttttctctat ttctgcgagt gcagttactg caagacaatt tcagagagag ttagatttgg
     1921 cagacaaagc aatattatgg gcactaatat cagccagtac taaagaggga cgaaaagcgt
     1981 gttcactaag ctattttgcc tgtaaggccg cagaggctga actcggactg gcatatatgg
     2041 ctgcgaatga taacaaggaa tttctcacat ccttatctaa tataatgagg tacaaaatag
     2101 atgctggact ttccgaatcc tatacatgct atttattaag taagggaaag atcataagac
     2161 catatctgaa aaatctaaat ccactccaac ttgccgctga ttgcattgaa acagtaaata
     2221 aaataaaaga taagaataaa aaaatcattg atataaattc agttaatatt tgtagtgatg
     2281 ataaaaatat aaaattgaga gttaatagta ctattatggc cattgatgat tctataaagt
     2341 gcatcgatga atgataactt aattttattt aaaaatatat taataactca ctccccagga
     2401 gtttttttaa cttagtttta taatgaatac gataattaaa agtgaagaag aaaatagttg
     2461 tatacatata ttccatgacc ctacattgtg atctatagat taaatatttt ttaaatgtga
     2521 ctgtcatcct tcagataata aagccatcat cgcaatagat ggaaaagcac tcctgctctt
     2581 ttttgattag agccgtcgca ggaggaacaa ctcatatcat cattgcgatc tcgacgatgt
     2641 atagtctcat cctgcggcag aacaagacgg ataaaaaatc caatgagagc acactcatcc
     2701 agaacttatt aacataatga atattaaaga aaaatcatca cagctaatga agtaatatgc
     2761 aaaaataatt agtccttaca aggagcaaag ttccataatc tcccccctcc ccctcaatga
     2821 tccaaataaa gataatgcat ccagctggtc attcttagca gcactttccg catcaccgaa
     2881 acatggctga aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca
     2941 tcgatatcat cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggcacg
     3001 accgttaatg attgcaatac cccctgcgcc tgataagaac cgcctggcac gacaggtaaa
     3061 atactagtca gtttgccgtg aaacacctgc ccaggtagcg cgttaaacac cacttccgca
     3121 tcatcgccgg gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt
     3181 ttttgctcgg ggatgaagac catcaccgga cgcagcggca aggcagctgc gtatgtacct
     3241 gggcggatca gtacctgagt aacgtagcca ttgctcggcg cgcgaatgac agtctgctca
     3301 aggttatatt ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg
     3361 ttaaccatac tgtcgagctg gctctggatc tgcgcctgct ccgccaccga gcctttcacc
     3421 agcgcatcct gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc cgagaacgga
     3481 ttcaccgccg cctggctgcc tttcaagtaa cgttgataat ttttaaacag acggtcgcgc
     3541 tccgctgaaa cctgggtggt gttggcctgc gcttcagtga gctgcgcacg cagcgtcttt
     3601 atattatgcg tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc ctggtaacga
     3661 accgggtcga gcttaaaaag cacctcgccc ttttgaataa gctgattatt cttgtcagtg
     3721 acttcagtaa caattcccgt cacctgtggc gtgataggga tcgctatcac tgccttttgc
     3781 gcggtaaaag tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac
     3841 acgcctccca gcgccgccgt cgccagcgtc cactgattta ccggaatgcg gaagatttta
     3901 aagaccgccc acgccagcgc cacgtaagtt aaaacaatca atagatccat aattagatct
     3961 ccgggaacgt ggaatagtca gtctttttct cggcggcgag ttgatgctcc agccgggaaa
     4021 ttcggtcaga aagtgcggcg atttccgggt cagtcgcttt ctcctgcgac gcaacatgtg
     4081 actgcatacc ccagccacgc tccggttgat agagcgtcgc ccagatccac agaaacggcc
     4141 agataacatg cagagtaaac aggctcaccc agcccgccgt atgaatagcg tcggcatggg
     4201 gatggttgcg ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa
     4261 aaatcaccag aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg
     4321 cctcggattc acgtttatca gtgttgtttt tgggctggca gccagaaggg agtcaggctg
     4381 atattttgac aataatccgg gttcgcgatt ctcgccataa caccaaagaa taatttttag
     4441 aggtgatgag ttgcttagtt acataacgat tgtatgacga aggcataaca tgctgtagat
     4501 cacatcaggt gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa
     4561 ccatcaaaac aaaaatgtga cactactcac atttaaatgc catttttagc gaaaatcgcc
     4621 gccttgttgc ttttttacac aagcgttttg tgatgaacgt cacgtcaatt acctctctac
     4681 cccctatatt tatgtgattg atatcacaca aaaggccgtc gactggacag ttaaccgatt
     4741 cagtgccaga tttcgcagta tctacaaggt ccggctacct ctgccgccac attaacaaaa
     4801 aacctcgggc ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc
     4861 gatattaaga tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat
     4921 atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta
     4981 ccgaacgaga cgctggcgaa gctggtcggg ccgatgatca cttatctcct gccgctgctg
     5041 atcggttata ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc
     5101 accaccatgg gcgttatcgt cggcgcagac atgccgatgt tcctcggttc tatgattgca
     5161 ggtccgctgg gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa
     5221 tccggttttg agatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct
     5281 attctggcat tcctcggcat tggcccgatt gttgaagccc tgtccaaaat gctggctgcg
     5341 ggcgttaact tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg
     5401 gcgaaaatcc tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggtatt
     5461 cagcagtccc atgaactggg taaatcaatc ttcttcctga ttgaagctaa cccaggtcca
     5521 ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg
     5581 ggcggtgcgg caatcatcca cttcctgggg ggtatccacg aaatctactt cccgtatgtg
     5641 ctgatgaatc cgcgtctgat cctcgcagtc atcctcggcg gtatgactgg cgtgttcacg
     5701 ctgactatcc tgggcggtgg tctggtttct ccggcatctc cgggttctat ccttgctgta
     5761 ctggcgatga caccaaaagg tgcttatttc gctaacatcg cgggtgtgtg tgcggcgatg
     5821 gctgtctcct tcgttgtctc tgctattttg ctgaaaacca gcaaagtgaa agaagaagat
     5881 gatattgaag cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa aggcacatct
     5941 ccgctgtctg cgggcgatgt gactaacgac ctgagccacg tacgtaaaat catcgttgcc
     6001 tgtgacgccg gtatgggttc cagtgcgatg ggcgcaggcg tgctgcgtaa gaaaattcag
     6061 gatgcaggtc tgtcacagat ttcggtcact aacagcgcga tcaacaacct gccgccagat
     6121 gtggacctcg tcatcactca ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag
     6181 gcgcagcata tttcgctgac caacttcctc gacagcggcc tgtacaccag cctaaccgaa
     6241 cgtctggttg ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga cagctttgac
     6301 gattccagtg ctaacctgtt caaactgggc gcggagaaca tcttcctcgg tcgcaaagca
     6361 gcaaccaaag aagaagcgat tcgttttgct ggcgagcagc tggtgaaagg cggttacgtt
     6421 gagccggaat acgttcaggc gatgctggat cgtgaaaaac tgaccccgac ttatctgggt
     6481 gagtctatcg cggtgccaca cggtacggtt gaagcgaaag atcgcgtact gaaaacgggc
     6541 gtggtgttct gccagtaccc ggaaggcgtg cgcttcggtg aagaagaaga tgacattgcc
     6601 cgtctggtga ttggtattgc agcccgtaac aacgagcaca ttcaggttat caccagcctg
     6661 accaatgcac tggatgatga gtccgtcatc gagcgtctgg cacacaccac cagcgtggat
     6721 gaagtgctgg aactgttggc aggtcgtaag taatccaatc ctaccctctc ctcacggaga
     6781 gggtttgggt gagggaaaag cctcacccca gccctctcgg gtaaaaacat tgatgaaggt
     6841 taatactatg aaagcattac attttggcgc aggtaatatc ggtcgtggct ttatcggtaa
     6901 actgctggca gacgcgggta tccaactgac gtttgccgat gtcaatcagg tggtacttga
     6961 tgccctgaat gcccgtcata gctatcaggt tcatgtggtt ggtgaaaccg agcaggtaga
     7021 taccgtttcc ggcgtcgatg ctgtcagcag cattggtgat gatgtcgttg atctgattgc
     7081 tcaggtcgat ttagtcacta ccgccgttgg cccggttgtg ctggaacgta ttgctccggc
     7141 tatcgccaaa gggctggtga aacgtaaaga acaaggtaat gaatccccgc tgaacatcat
     7201 cgcctgtgaa aacatggtac gcggtaccac gcagctgaaa ggccatgtga tgaacgccct
     7261 gccggaagac gccaaagcgt gggtagaaga acacgttggc tttgtcgatt ccgccgttga
     7321 ccgcatcgta ccgccttcgg cttcggcaac taacgatccg ctggaagtga cggtagaaac
     7381 tttcagcgaa tggattgtcg ataaaacgca gttcaaaggc gcactgccga acatcccagg
     7441 catggagtta accgacaacc tgatggcatt tgtcgaacgt aaactcttca ccctgaacac
     7501 gggtcatgct ataaccgcgt acctcggaaa actggctggt catcagacca ttcgtgacgc
     7561 tattctcgac gagaaaatcc gcgcggtggt aaaaggtgcg atggaagaaa gtggtgcggt
     7621 actgatcaag cgctacggct ttgacgcaga caaacatgcg gcgtacatcc agaaaatcct
     7681 cggtcgtttt gagaacccgt atctgaaaga tgatgtagag cgcgtaggcc gtcagccgct
     7741 gcgtaaactg agtgctggcg accgtctgat caagccactg ctcggtacgc tggaatacag
     7801 ccttccgcac aagaatctga ttcaggggat tgctggtgca atgcacttcc gcagtgaaga
     7861 tgatccgcag gctcaggaac tggcagcact gatcgctgac aaaggtccgc aggcggcgct
     7921 ggcacagatt tccggtcttg atgccaacag cgaggttgta tccgaggcgg taaccgctta
     7981 taaagcaatg caataatggt ggaccaggcg caggacaccc tgcgcccgaa taacagattg
     8041 tcagatatgc aggcaacaat ggaacaaacc caggcctttg aaaaccgtgt gcttgagcgt
     8101 ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg ccgtcgagct tttgaccgag
     8161 gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg attacgcggt gaagtatgct
     8221 gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc tttctgtgcg tttaaaactc
     8281 atttacgggt tgggcgtcat taaccgccag gaatacgaag atgcggagct gctgatggca
     8341 ttgcgtgaag aactaaatca cgacggcaac gagtacgcct ttaccgacga cgagatcctt
     8401 ggaccctttg gtgaactgca ctgcgtggcg gcgttaccac cgccgccaca gtttgaacca
     8461 gcagactcca gtttgtatgc aatgcaaatt cagcgctatc aacaggctgt gcgatcaaca
     8521 atggtccttt cactgactga gctgatttcc agaatcagct taaaaaaagc ctttcaaaag
     8581 taagcaacgt ctgcttactg cccctctacc tgcttcggcc gataaagccg acgataatac
     8641 tccagacgtt gtagatataa cggcacgctt tcatcgggta ttccggacgg aatcgcgtta
     8701 cggggaggaa gttttttcag atactcccgg aacgcctggc ttgatgccat gaaatctacg
     8761 gctttatcga taagaagcgg aacgttttca cctaatttgc ccatgatgat atctccgtat
     8821 taccccgcgc cgggaatgcg cggccgccaa ttttagttta ggttcccggt aaagatgaca
     8881 ttaaggaaaa cgtgctgaat cctcaataat catccgctgc tgtttattat cttttttcta
     8941 tagatttctt aattaatcaa taaattgcgt agtttttaag catgttaatg attacgttac
     9001 agaactattg caatgtcatc agttgtgaac aaagcacctg gtcgcgcata ctaggggcta
     9061 taaatttatc tttatcagaa gccatcacat gaaagaagtc gaaaaaaacg aaatcaaacg
     9121 tctcagcgat cgtctggacg ccatccgcca ccagcaggcc gatctgtcgc tggttgaagc
     9181 cgcagacaaa tatgccgagc tggaaaaaga gaaagccacg ctggaagcag aaattgctcg
     9241 cctgcgtgaa gttcatagcc aaaaactgag taaagaagca cagaagctga tgaagatgcc
     9301 gtttcagcgc gcaattacca aaaaagagca ggctgatatg ggcaagctga agaaaagtgt
     9361 tcgcggactg gtggttgtgc acccaatgac cgcgctgggc cgtgaaatgg gcctgcagga
     9421 gatgactgga ttctctaaga ctgcgtttta agcgcctcag acgactggtt cgatcatatc
     9481 aacccgcatc tggcttgcct tagctggtca atgcggggac tctcctgtct actcgcctaa
     9541 ttaccaaaca attcactccc tttacttcga tttaccgctg gttgatattc tgaaaatgca
     9601 tttttaccga aatagccatc tataaacaat aaaaatatat agaagatatc atttgttaat
     9661 taacaagcat ttacgtctct ttttttcgga tttaattgtg aaattttaat catcatattc
     9721 acaaaatcaa ttatctgtaa aatttttccc agacatggtt tctttcatat ttgacattta
     9781 ttaataatca tcattagaat ccacgaaaaa taatatattc atacaaacac agacatcttt
     9841 tgaaacgaat gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc
     9901 actgtcatta atgtaaagca acatagctca ccaataatac aatggacaac tctaccggca
     9961 ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag
    10021 tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa
    10081 gccagaaagt agtttactga aacaacaaga agaacataat aataaaattg tgctacttga
    10141 aaaacaacaa gcacagctaa agagtcaact cgaaactatt caaaaacaac aaactggcat
    10201 aataaacagt acaaagactt taactcacgt aattaaatcc gttaaagacc aacaaaatac
    10261 ttttattttt accgagttta acccagcaaa aacgaagtat ttcatcctga acaacggttc
    10321 ggtggcttta gcgggtcgag tgttatccat tgacgccaca gagaatggta gtgttattca
    10381 tatctcactg gtcaacttat taagtacacc tatctcaaac attggtttta atgcgacatg
    10441 gggtggtgaa aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa
    10501 cacatcaatg aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac
    10561 cctgaagggt gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa
    10621 tattcagttc gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat
    10681 gcagttagta cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag
    10741 ttatctggaa ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg
    10801 gcaagaaatc tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt
    10861 cgtcgtttgg ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta
    10921 gtggatcagc tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta
    10981 tgaacaccag tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata
    11041 gctctgccat cgggtcaaaa acccatgcga ttggcggtgc atcaatggcc tttggggtta
    11101 gtgcaatatc agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc
    11161 aatactcaat ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg
    11221 gggactcttc caaagcggaa ggagcaaacg ccattgccct gggaaatgcc actaaagcta
    11281 ctgagattat gagtattgct cttggcgaca ccgccaatgc gtcaaaagcg tattcaatgg
    11341 cgctgggagc aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg cgtagcagtg
    11401 tagctagcgg tactgacagc ctcgcatttg gcagacaatc acttgccagc gcagcgaacg
    11461 ctattgcgat aggtgctgag accgaagccg ctgaaaatgc aactgctatt ggcaataatg
    11521 cgaaggcaaa agggactaat agcatggcaa tggggttcgg aagccttgcc gataaagtca
    11581 atactatcgc attaggaaat ggcagccagg ctctggcaga taatgcaatc gccataggcc
    11641 agggcaacaa agctgatggc gtggatgcca tcgctctggg taatggtagc cagtcgagag
    11701 gcttaaacac cattgcctta ggcacagcca gtaatgcaac tggtgataag agtcttgcgc
    11761 ttggtagtaa tagcagtgcc aacggtatta actctgtcgc gctgggcgca gattccattg
    11821 cggatttaga caataccgtc tctgtcggca atagttcatt aaaacgcaag atcgttaatg
    11881 tgaaaaatgg cgcgatcaag tctgacagtt acgatgccat taatggttca cagctttatg
    11941 ccattagcga ctcggtagca aaaaggcttg gaggaggggc tgcagtagat gttgatgacg
    12001 gtactgttac agcaccaacc tacaatttaa aaaatggtag caaaaataac gtaggggctg
    12061 cgctcgctgt acttgatgaa aacaccctgc aatgggacca aaccaaaggc aaatacagcg
    12121 ctgctcatgg tactagtagc ccaactgcca gcgtaatcac cgatgttgcg gatggcacga
    12181 tttcagcctc cagtaaggat gcggttaacg gttcccaact gaaagctacc aatgacgatg
    12241 tcgaagccaa caccgccaat atcgctacta ataccagcaa cattgccacg aatacggcaa
    12301 atattgccac caataccacc aatatcacca acctgacgga ttccgttggt gaccttcagg
    12361 ctgatgccct gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata
    12421 ccaccagcaa aatcaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg
    12481 ttaacggctc tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg
    12541 ccaataacac ttccaatatt gccactaaca ccaccaacat ctctaacctg actgagacgg
    12601 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgta ttcacggcag
    12661 ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac ctgacgactg
    12721 gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga
    12781 ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta
    12841 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg
    12901 gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt
    12961 ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg
    13021 gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac aaaatcggtg
    13081 aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt
    13141 ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta
    13201 ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt
    13261 ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg
    13321 ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta
    13381 atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact cttgacgacg
    13441 ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag
    13501 ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg
    13561 acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg
    13621 gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc attgcgaacg
    13681 ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg
    13741 atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca
    13801 tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt
    13861 ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg
    13921 ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac tatgtgcgta
    13981 ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc gctacagcta
    14041 taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag ggcagctaca
    14101 gcgacgttga tacgggtatc gccctgggta gcagctctgt ttccagccga gtgattgcca
    14161 aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac gacaccacgg
    14221 atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt caaatcatca
    14281 acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg cagaatgcga
    14341 ttggtgcggt cgcaaccacg ccgactaaat acttccacgc taattcaacg gaagaagatt
    14401 cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg aatggcgata
    14461 aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac ggcattgcca
    14521 ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat ggttctacga
    14581 ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg cagaactctg
    14641 tcggtgaatt ctcagtcggt agtgcggatg gtcaacgtca gatcactaac gtcgcagcag
    14701 gttcggctga taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat gcgcaggttt
    14761 cccagaatac ccagagcatt actaacctgg ataatcgggt aacgaatctt gattcacgcg
    14821 tcaccaatat cgaaaacggt attggcgata tcgtcaccac cggtagcacc aagtacttca
    14881 agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtc gcgattggtt
    14941 ctggctccat tgctgccgcg gataacagcg tcgcactggg tacagggtct gtggcaaccg
    15001 aagaaaatac gatctctgta ggctcatcta ctaatcaacg tcgtatcacc aacgtagccg
    15061 caggtaaaaa cgacaccgat gctgttaacg tggcacagtt gaagtcttcc gaagctggcg
    15121 gtgtgcgtta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg
    15181 gcggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg
    15241 cggtgaatta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc
    15301 gaatggttga gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg
    15361 cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc agcatggctt
    15421 ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga
    15481 gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact
    15541 ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg
    15601 caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa
    15661 caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca
    15721 caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta
    15781 aaactcacat caatattgcc aatacataac atttagttaa ccattcattg tcattatccc
    15841 tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag
    15901 actcattaca cgatgtgcgt ggactccagg agacctgcaa tgaatctctg gcaacaaaac
    15961 tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt
    16021 ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta
    16081 gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc
    16141 tcggtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca
    16201 gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt
    16261 ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct
    16321 ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc
    16381 ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca
    16441 gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac
    16501 agctttgaga ttggccagat ggtggggcgg cagctaccgt ttatgaccat tatcgtgctg
    16561 ttctggatca tggcgattat ggacggctgg cgcggtatca aggagacgtg gcctgcggtc
    16621 gtggttgcgg gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg
    16681 gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa
    16741 cgctggcagc cagtgcgcgt attccgtttt ggtgatttgg gggcgtcaca ggttgatatg
    16801 acgctggccc acaccggtta cactgcgggt caggtgttac gtgcctggac accgttcctg
    16861 ttcctgacag ctaccgtaac actgtggagt atcccgccgt ttaaagccct gttcgcatcg
    16921 ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt
    16981 gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac
    17041 tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg
    17101 aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctctg
    17161 cccatatact ccatcggtat ggtgctggca ttcgccttta tttcgaacta ttccggactg
    17221 tcatcaacac tggcgctggc actggcgcac accggtcatg cattcacctt cttctcgccg
    17281 ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc
    17341 gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc
    17401 gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc
    17461 tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac
    17521 agcctgatct tcacctgtat agtgggcgtg atcaccacgc ttcaggctta tgtcttaacg
    17581 tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg
    17641 tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc
    17701 aactggcgat gcaactcggc gtatcacgta attcactgcg cgaggcgctg gcaaaactgg
    17761 tgagtgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg
    17821 acacatggtc ggagcaaaac atcgtccagc cgctaaaaac actgatggcc gatgatccgg
    17881 attacagttt cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg
    17941 cggcaatgcg cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc
    18001 taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg
    18061 aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat
    18121 cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga
    18181 ccgaacaaca tcaggctgtc attgacgcca tttttgccgg tgatgctgac ggggcgcgta
    18241 aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc
    18301 aggctcgcca cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga
    18361 aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc
    18421 gccgttcctg ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa
    18481 cgtggaagat ttgtcagaag tggcgctgcg ccagcgtatt ctgaaaaaca tgtccgactt
    18541 aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtggcgc taggtccggt
    18601 aggtttgtgt ggcatgtatg cgcgacgcgg cgaagttcag gcagccaaag cggcggatgc
    18661 gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc
    18721 agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg
    18781 taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat
    18841 gccgacaccg ggagcgcgtt atcgtgatgc gcattctgga atgagcggcc cgaacgcggc
    18901 aatgcgccgc tatttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa
    18961 cggtcgtccg catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga
    19021 agattacatc ggctggctgg gaaataactt cgatccgtcc atctcatgga aagaccttga
    19081 gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga
    19141 tgcgcgcgat gcagtacgtt ttggtgctga tgggattgtg gtttctaacc acggtggccg
    19201 ccagctggac ggtgtactct cttccgctcg tgcactgcct gctattgcag atgcggtgaa
    19261 aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat
    19321 gattgcgctc ggtgccgaca ccgtactgct gggtcgtgct ttcttgtatg cgctggcaac
    19381 agcgggccag gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc
    19441 gatgacgctg actggcgcga aatcgatcaa tgaaattacg caggattcgc tggtgcaggg
    19501 gctgggtaaa gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc
    19561 gtttgagccc cctcacccta accctctccc tcagggagtg gggaccgttc ggcgctgtat
    19621 gtactccctc actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggtg
    19681 aggggaaaag gccgcactgc tcaccaattt tctgctatcc tgcccccgca ctaagggggc
    19741 agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg caatatcatc
    19801 cgtctttgcg ctaataccgg ctttcgtctg catatcatcg aaccgatggg atttgcctgg
    19861 gacgataagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt
    19921 catcatgact ttcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc
    19981 acgaaaggta cgcctgctca cagcgccgta aactatcagg atggcgatta tctgatgttc
    20041 ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa
    20101 attcgcattc cgatggtgcc ggacagccgc agcatgaacc tgtccaatgc ggtgtcagtt
    20161 gtggtgtatg aagcctggag gcagctggga tatccgggag cgttgttgag agattagtta
    20221 ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat cagatcccat
    20281 ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc attgatggct
    20341 tatcgctgtc tggtttaccg acaatacgag ccggaacgcc agcggcggtt gtatgcggcg
    20401 gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgacca acttcaatat
    20461 tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc gggtgacggt
    20521 caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat acgtcgtttt
    20581 caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata atgccacggc
    20641 caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg ttttgcagaa
    20701 agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga taggcctgca
    20761 aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact gccgggtcgc
    20821 gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg tcagcggcgt
    20881 aggcttcttc caccacttca cggatagcaa tagcaggcat aattggcgat gacagcttgt
    20941 tcgccagcat gtagctcagt gcactaccaa ggttttcgtg cttgagtagc gtcgcgtggt
    21001 aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct ttaatattgt
    21061 tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga gataatgacc
    21121 ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg ttagtggctg
    21181 ctgcgctcgt ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg cgcgtttttt
    21241 ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc gaagcgatgc
    21301 gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc aatcttctcc
    21361 tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg gttacgcgac
    21421 tggttgtcgg tacaggtaag caccagatcg ccaagccccg ccatgcccat aaaggtggca
    21481 ggatcggcac ccagcgccgc accaagacgc gacatttcag ccagcccacg ggtgatcagc
    21541 gccgtacgcg cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc aatggcaatg
    21601 acgtttttca ccgcaccgcc aagctgcaca ccgatgaaat ccgggttgct gtaaacgcgg
    21661 aaacttttgc cacagtgcag caattgttgg agatcatcgg caaaggtctg gtcggtagag
    21721 gccaacgaaa tagccgtcgg taaacctgcc gccagttctt tcgcaaacgt tgggccagag
    21781 ataacagcca gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg taacagacgt
    21841 ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg acgcatcagc
    21901 ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac gagaatatta
    21961 cggctggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc
    22021 acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg
    22081 tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc aagagcggtg
    22141 ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat caggcatcct
    22201 gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac agcgcatcga
    22261 agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg ctggtgatgc
    22321 actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct
    22381 gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca cacaggaacg
    22441 cggtttcttc gcccagggag gccgttacgg taacacgcag taccacttcg tatacgtcat
    22501 ctgccagttg ggaagatgcc gtatccagat caagtttaac ttctggttgc caatctttct
    22561 ggaaaacgtg cggcgcgttc ggcgcttcga aagagatatc cttggtataa atacgttgga
    22621 tctggaaagt catttcagtg ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg
    22681 tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca ggggatccag
    22741 tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct gtgcgtcaat
    22801 aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac gcttggcggc
    22861 attgccatcg atcggcagct cctggaaact cacgcccttg ctgctcagca gtgcttttgc
    22921 acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca taaattagct
    22981 cctgtttttt taccctgaaa tttcatgttg cagggcggca gcaaaatgga gtgtccccag
    23041 aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc atcatgaaag
    23101 acgacaggta aattatttgc cgcgcaccag aggcaagttt tcgccagccc agccagcaac
    23161 gccctctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcgt ttgcaggctc
    23221 ctggcactgc atgccagaac cgtctaccac gataaccggt ttgtctttgt gcttctcaag
    23281 ctcaccaaca ttgttggctt tgatttcact cggcaacaga ttgatagaac ctgcgatatg
    23341 gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt ctttgttgat
    23401 cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc tcttaaacgt
    23461 agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc
    23521 aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg attaaaaaaa
    23581 ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta actggaatgc
    23641 aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt
    23701 cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat
    23761 tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt
    23821 gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt ttagtgctaa
    23881 aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg acgcttccgg
    23941 tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc acgttaacct
    24001 gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa tcaaagatcg
    24061 cgctttcttt gctaatccag tgctgactgg cgcggtagat aaagcgaaaa acgcaggtaa
    24121 agcagtacac attatgggtc tgctctccgt aggcggcgta cacagccacg aagatcacat
    24181 catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt
    24241 ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga
    24301 gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt actacgcgat
    24361 ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga ctctggcgca
    24421 gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga
    24481 aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat
    24541 ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc gtgaaatcac
    24601 tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg ttaacgtcga
    24661 tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt acccacccgc
    24721 ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat
    24781 ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg tagaagagtc
    24841 gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct acgatctgca
    24901 accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca agagcggcaa
    24961 atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca ccggggtgat
    25021 ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc
    25081 ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg ctgaacagat
    25141 gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag ttccgctgat
    25201 ttatgttggt gataagaacg tgaaagcggt tgaaggcggc aaactttctg acatcgcgcc
    25261 gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta agccgctgtt
    25321 catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga cacgggccgt
    25381 gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta gcgctggcgt
    25441 attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat ctattcaggc
    25501 cgatatcgcc gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct
    25561 cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac
    25621 gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt cgattgccaa
    25681 actggagcag caaaaagccg cccaggagcg cagcctcgcc gcgcaactgg atgccgcatt
    25741 ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa gccagcgtgg
    25801 acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa ccattgccca
    25861 gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag agaaacaaag
    25921 cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga ctcaggcgct
    25981 gaacgagcgt aaaaagacgc tggcagggct ggagtcttcc atccagcaag gtcagcaaca
    26041 gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac agcattgccc gtgcggaagc
    26101 cgcggcgaaa gcgcgtgcag aacgagaagc acgtgaggcc caggcggttc gcgaccgcca
    26161 gaaagaagcg acgcgtaaag gcaccaccta caaaccgact gaaagcgaaa aatcgctgat
    26221 gtcccgtacc ggtggtctgg gcgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc
    26281 gacgctgcat cgctatggcg aacagctaca gggtgaatta cgctggaaag ggatggtgat
    26341 tggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga ttctggctga
    26401 ctggctgcaa ggctacggtc tggtggtggt ggttgagcat ggtaaaggcg acatgagtct
    26461 ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg cgggccagcc
    26521 aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt tcgaaattcg
    26581 ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt tgtttccatt
    26641 tcgtcgtaac gttcttgcat ttgccactct gttggcgctc tcctcccccg tacttgctgg
    26701 caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa accaggtgct
    26761 ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg ccagagagat
    26821 ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctgccaa tggcaccgtt
    26881 gagtaaacag ccgctggaga aaaatacgct gcgcccggag atgagcagcg acgaaattga
    26941 gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca acaaccacat
    27001 gggtagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg cgctggagcg
    27061 ttacaatctt tacttcctcg acagtgtaac cattggtaat acccaggcga tgcgcgccgc
    27121 gcaaggcact ggcgtgaagg ttattaaacg gaaggtgttc ctcgacgatt cacagaacga
    27181 agcggacatc cgtgtgcagt tcaatcgcgc aattgatctg gcgcgtcgca acggttcaac
    27241 cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga tggtttataa
    27301 cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga
    27361 tacgtctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt
    27421 gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct tctttgaagt
    27481 attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc agtggcaagg
    27541 ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc
    27601 gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa agaccacagt
    27661 aacgcctggt aacccacctt cacgctgcgc acattggtaa tcaggcgctt atacataccg
    27721 gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc tttgcgcacc
    27781 gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg ataaatcgta
    27841 attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt aatatagtga
    27901 cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga cgtattatgc
    27961 agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcatt aaacatgaat
    28021 tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat gttattttta
    28081 acaataacat cgcggcgata aacccccatc cagacaacgt gagtccaacg gcgcgaagaa
    28141 agccccatcc gcagccagtc cgggccggtt aatacaccgg ttgagcgaag gcgatcgctg
    28201 gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt gcactgcgcc
    28261 acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt cgtacatggt gggatagacc
    28321 tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccacctcgat cccacgatta
    28381 cgcgctaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg atagttttct
    28441 gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat aataatgatt
    28501 tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa atcatcgccc
    28561 gcattatata acggaataat aacactaagt ttactgggct gttcatcata aattaccgac
    28621 gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat taagcctgtg
    28681 agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg agaacagcta
    28741 tataagagat cagcgcaatt acgaactctt tattacactg acaggatttt acatatttaa
    28801 cggattatcc tcaggcaaaa gggctggaat accagccctt gttcgtgtta atcccagctc
    28861 agaattactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg gaaatcatca
    28921 atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat cagcgccgcc
    28981 atcttgtacc aggtttcaaa catctcacga ccgtaaatac ctttaatgaa caagccttta
    29041 aagatcactt tggtccagtc gatagacata tcagacggcg gaatacccag catcgcaata
    29101 cggccgccgt ggttcatggt gtcaagcatg gtacgaaacg ctggcggcgc accggacatt
    29161 tccagaccga catcaaagcc ttcggtcatg cccagttctg ccatcacatc attaaggttt
    29221 tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc aaggcggtat
    29281 tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac cgccgcagcc
    29341 ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag atcgaacgac
    29401 agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc gtcggagata
    29461 ttgtctggga ttttgaaggc gttgaacgcc gggatcacca gatattcggc gaagcagccc
    29521 ggacggttaa cgccgacgcc gatcgtgttg cggcacagat gggtacgacc gccacgacag
    29581 ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc gattttgaag
    29641 cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc tacgaccatc
    29701 ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac gtcagtcccg
    29761 cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc cggtacagga
    29821 acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt catctcacat
    29881 cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac tgcacgcgta
    29941 atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg acctttcgga
    30001 acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc acgggcaaat
    30061 ttctgcgcca ctactgcatc gccaagcatg accggaataa tggcatgatc ggctcccgcc
    30121 agggtaaagc ccgccgccga catttgctca cggaactggc gcgcgttcgc ccacagacgg
    30181 tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt tgatggacgc ggcaacaatg
    30241 gccggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag ccactcaacc
    30301 acctctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt accaagcgta
    30361 ccggtgataa tatcgacccg gcccatcaca tcgcagtatt catgggaacc acgaccattt
    30421 tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc gtatttatct
    30481 gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa tacaccatcg
    30541 gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg agcttcaagc
    30601 tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg cacaccatca
    30661 ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc cagcagcgtt
    30721 tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc ttccatcccc
    30781 aggaaggccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa
    30841 cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgccgc cgcaatcaga
    30901 tcaggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac gtggcttcca
    30961 tcagccacag tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac
    31021 ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc tccacgcatt
    31081 gcgattctcc agacagggca aattccagca catattaccc aaacttatag gtcgcgacga
    31141 gatgacgcgt taacacttct gcaaaattca ggataaataa cgtgtaactt gatgtctctc
    31201 aacacctaca gatagtagaa aaactgtaca gttttggctg ggcataatga tgattgattt
    31261 tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct atctcagttg
    31321 gtatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga aaattctcag
    31381 cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat cgaaccattt
    31441 tcctctcccc agatagttta ggttgaagag atcggggcgt ttgtaatagc acatgacaaa
    31501 aatgccctgg tcatcatcca caatgttatt atttagtgtt attttttgac tttccagaac
    31561 taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc caataatata
    31621 tacatgattg ccaatcatga aatcaaaaac ctgttgttga gatgtgacag taagcccttt
    31681 ttttatagtg aaaagatgca tcttactttc atcaaatggg aaatcccaaa ttttcaatcc
    31741 tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa gaggcgtttt
    31801 cactaagccc atgttgatag ctttatttac aaaataggcc ttaaggttac ataccaaaac
    31861 atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc tatttgtaaa
    31921 cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt tcttttttat
    31981 atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaaccc ttggtttcaa
    32041 atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct caaagtaact
    32101 aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag cagtccaatc
    32161 ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca ttactaataa
    32221 gaccattaat ataaaaaaag aattatatca taaatattaa cagaatgtat actgtatatt
    32281 atccattcaa atgcttccgt tatttatcat acatcggaat attgatacta aagcactatt
    32341 cacatgcaaa accaacatcc gccatgaagg actagctaaa acccaaacta gtttgttgca
    32401 attagcatcc ttgcacctct atgtaaaggg ctgaagggat tcggatgtga tggtatgatt
    32461 acagacattc gtgtctgaga ttgtctctga ctccataatt cgaaggttac agttatgatc
    32521 atcgttaccg gcggcgcggg ctttatcggc agcaacatcg ttaaagccct gaatgataaa
    32581 ggcatcaccg atattctggt ggtggacaac ctgaaagacg gcaccaagtt tgtgaacctg
    32641 gtggatctga atatcgcaga ctatatggat aaggaagact tcctgatcca gattatggct
    32701 ggcgaagagt tcggcgatgt cgaagcgatt ttccacgaag gcgcgtgctc ttccaccacc
    32761 gagtgggacg gcaagtatat gatggataac aactatcaat actccaagga gctgctgcac
    32821 tactgcctgg agcgtgaaat cccgttcctg tacgcttctt ccgcagccac ctacggcgga
    32881 cgcacctccg actttattga atcccgcgag tacgaaaaac cgttgaacgt ctacggttac
    32941 tcaaaattcc tgtttgatga atatgttcgc caaatcctgc cggaagcgaa ctcgcagatt
    33001 gttggcttcc gctatttcaa cgtttatgga ccgcgtgaag gccataaagg cagcatggcg
    33061 agcgtcgctt tccatctcaa cacccagctt aataacggtg aatcaccgaa gctgttcgaa
    33121 ggtagcgaga actttaaacg cgacttcgtc tatgttggcg atgtggcaga tgtaaacctg
    33181 tggttcctgg aaaatggcgt ttccggcatc ttcaatctcg gcaccggtcg tgcggaatcc
    33241 ttccaggctg tagctgatgc tacgctggct tatcacaaga aaggccagat cgaatacatt
    33301 ccgttcccgg ataagctgaa aggccgctac caggcgttca ctcaggcaga tctgacaaat
    33361 ctgcgcgcgg cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt aacggaatac
    33421 atggcctggc tgaatcgcga cgcataagag ctctgcatga aaatactggt gatcggcccg
    33481 tcttgggttg gcgacatgat gatgtcgcaa agtctctatc gcacgctcca ggcgcgctat
    33541 ccccaggcga taatcgatgt gatggcaccg gcatggtgcc gtccattatt atcgcggatg
    33601 ccggaagtta acgaagctat ccctatgcct ctcggtcacg gagcgctgga aatcggcgaa
    33661 cgccgcaaac tgggtcatag cctgcgtgaa aagcgctacg accgcgccta cgtcttacct
    33721 aactccttca aatccgcatt agtgcctttc ttcgcgggta ttcctcatcg caccggctgg
    33781 cgcggcgaga tgcgctacgg tttactcaac gatgtacgcg tgctcgataa agaagcctgg
    33841 ccgctaatgg tggaacgcta tatagcgctg gcctatgaca aaggcattat gcgcacagca
    33901 caagatctgc cgcagccatt gttatggccg cagttgcagg tgagcgaagg tgaaaaatca
    33961 tatacctgta atcaattttc gctttcatca gaacgtccga tgattggttt ttgcccgggt
    34021 gcggagtttg gtccggcaaa acgctggcca cactaccact atgcggagct ggcaaagcag
    34081 ctgattgatg aaggttatca ggtggttctg tttggctcgg cgaaagatca tgaagcaggc
    34141 aatgagattc ttgccgcttt aaataccgag cagcaggcat ggtgtcggaa cctggcgggg
    34201 gaaacacagc ttgatcaagc ggttatcctg attgcagcct gtaaagccat tgtcactaac
    34261 gattctggcc tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc cctgtatggt
    34321 ccaagtagcc cggacttcac accgccgcta tcccataaag cgcgcgtgat ccgtctgatt
    34381 accggctatc acaaagtgcg taaaggtgac gctgcggagg gttatcacca gagcttgatc
    34441 gacattactc cccagcgcgt actggaagaa ctcaacgcgc tattgttaca agaggaagcc
    34501 tgacggatgc gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct ccatacgttg
    34561 cccgcactca ctgatgccca gcaggcaatc ccagggatta agtttgactg ggtggtggaa
    34621 gaagggttcg cacagattcc ttcctggcac gctgccgttg agcgagttat tcctgtggca
    34681 atacgtcgct ggcgtaaagc ctggttctcg gcccccataa aagctgaacg caaagcgttt
    34741 cgtgaagcgc tacaagcaga gaactatgac gcagttatcg acgctcaggg gctggtaaaa
    34801 agcgcggcac tggtgacacg tctggcgcat ggcgtaaagc atggattgga ctggcaaacc
    34861 gctcgcgaac ctttagccag cctgttttac aattgtaagc atcatattgc aaaacagcag
    34921 cacgccgtag aacgcacccg cgaactgttt gccaaaagtt tgggctatag caaaccgcaa
    34981 acccagggcg attatgctat cgcacagcat tttctgacga acctgcctac agatgctggc
    35041 gaatatgccg tatttcttca tgcgacgacc cgtgatgata aacactggcc ggaagaacac
    35101 tggcgagaat tgattggttt actggctgat tcaggaatac ggattaaact tccgtggggc
    35161 gcgccgcatg aggaagaacg ggcgaaacga ctggcggaag gatttgctta tgttgaagta
    35221 ttgccgaaga tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa atttgtagtg
    35281 tcggtggata cggggttaag ccatttaacg gcggcactgg atagacccaa tatcacggtt
    35341 tatggaccaa ccgatccggg attaattggt gggtatggga agaatcaagt agagtgtcgt
    35401 tctacaagta tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa tttaaatctt
    35461 gaaataataa ccaataagtt gacatcggag ataagatgac ctcaacatta tttttctctc
    35521 tcgagaaaaa aaactggata gcgtactgga acagagctct cgtattctta ttcattacca
    35581 cctatttttt gggtgggatt acaaggtata aacatcttat tgttattctt atgacaataa
    35641 cgacaatcgt ctatctctgc aaacggccaa aacactatct ctcactattt aaaacatttc
    35701 tttttggtag tgttgccata ttaactattg ctgcattgct gtcacttctt caatcccctg
    35761 atgcaggtgc tagcatgaag gaaattttca aagctattat tgagaatact ttactatgca
    35821 caatagcaat accgatcata ttgagagacg agaaaagaga agatgtcgaa aaaatcgttt
    35881 tcttctcatt tattagtgcg ttgggcttac gctgtttttc tgaattgatt acctattata
    35941 aggactatca acaagggata atgccattcg cagattatag acaccgtagc atttctgact
    36001 cgatggtctt tttattccct gcattgttaa atctctggct tatcaaatca gcaaaatacc
    36061 gcatttcttt tgtggttcta agcgttattt ttatttttct gatattagga actttatcca
    36121 gaggggcctg gctttccgtg ttagtcattg gattaatatg gattctgatg tttaaacaat
    36181 ggaagttact attagtagga gtaatggttg ccatcattgc attgtcggtt attttcacac
    36241 ataaggagat gaccgcaaag ctaacgtata aacttcaaca aactaatagt tcttatcgct
    36301 atgcaaatgg tactcaaggc agcgcactcg atctaatatt agaaaatcct gttattggtt
    36361 atggttacgg taacgttgca tataaagatg tctataataa acgtgtcatt gattatccag
    36421 aatggacctt tagacaatca atagggccac ataattttgc gctattcatc tggtttggca
    36481 ctggtttatt agggctggta agtcttatga tgctatactg tgcaatattg aaagagtgta
    36541 taaaaaatgg cgtcaagaat aaatatcgct caccatataa tgcatattat ataatcttac
    36601 tatcttttat aggttatttt gttatccgtg gaaacgtaga acaaattgaa ccaaatttat
    36661 taggcgttta cgccggctta ttattagcga tgaaaaacaa gtaaaaaata aaaaaggctg
    36721 cataatgcgg cctttttatt tattcaaacc aattatgaat aacctcttcg aatctttgag
    36781 ttacgccatc ccaactgtag tgttcaaaca caaagtcctg accttgcttt gcaactgctg
    36841 ttaattcagg attcgctaac gtttttaaaa tatcgctgct tatggagtct gcagtcatag
    36901 gttcttttaa atggaatccc gtagtatttt ctttgacaaa ttccgtcatc cccccacgcg
    36961 tactcactaa aacaggctta ccagcaccca tagcttcaat tgccaccatg caaaatggtt
    37021 cctggaactg ggaaggaatg acgactaaat ctgccagtgg gtaataacaa tgcatctttt
    37081 caggcggtat gcttcctaac ataatgcaac gatccttcaa acgttttgca atttccctga
    37141 cattgcgttg atatgcacct ttgtcacttt tactcatttc tgtataatca ccaatcacaa
    37201 ctaattttaa atttttgtga gcagcggcca atttttcgaa tgattgcatc aacagcaata
    37261 tgcctttgtc agggacgata cgcccagcat aaaaaataat tttctcttca ggggagatat
    37321 taagttcgct tctagtgatc ggctgaaaat tagattgata ggtttctaaa tcaattccat
    37381 ttggtacaat ctcaatatct gcatttgcta aataagattg gtaatacttt ttcaagtata
    37441 aactaggcac tatcatttta acattttgct caagcaattt tggttcaaat gcattatgca
    37501 tatgtataac cactcttgcc tgaggagctc gcttacgaat ttgagtgtat agcttcatgc
    37561 tattatgaac tacaataaca ctttctttcg ttataggaaa atcatgtgca atattcaata
    37621 ttctctgtga atatggaaga ggatcaagtc ttgtccattt ttgaaataac cgcttatata
    37681 tgcgactgaa tccaacccta tgaatactac aattatcatt aacaaaggtg taatttgagt
    37741 atcctggatt tttgatacaa acaatacgat ttggaaaatt ggtacgctga gcaacctgat
    37801 acatccacgt ttcaacagca gccgctccgc gaggaggaat tgaaaaaata ggagtaaccg
    37861 taaatattat tttatcaacc atctcgtata ttaacctttc ataacattat atttaattgc
    37921 tgtaaaaaca ccatcgagaa attttttctg gtaaagcaag tgtttatatt tttctttgta
    37981 ctcatgtttt ttaacagctt ttttataagg tatatttctc catggtgata tattataaat
    38041 attacgaaaa taatctgcag aggcgtagtt cgcccaatca tgccatggct tagttatacc
    38101 agtataatgt atgaagacag tgtcatcatt aataaaccgg gtgtaatatt cactattttt
    38161 ttcttcaaat tctgacttta tagtataaat acaattatat tttctcggta atattttagc
    38221 atgatgtaga aacattaagt ttaaaatatc ttgatccggg tactttaatt tttttataat
    38281 tgactcatct gataatagat caaagaattt ttccgttagt ctttgtttta accattcccg
    38341 taaattaata tacattacac cagagttaaa atagctaccg ttaaaatcct cattacacaa
    38401 acgctcagcg cttttacttt gcatagcatc tacatcaatt accactgccc catattcatc
    38461 tttaaactct aatgctatta actcgttcaa tgaaccctta cagacgatat cagcatcaag
    38521 atataataat gaatccaatc gcgcagaaaa ataatcaaat gaaagcaaac gataataaat
    38581 agatactggc cagatatttg attgaggtag cgccttcaat ggctcagaat caatatgata
    38641 taatttaatt actgtttgat acttcgttgc taattgtgat aataattcaa tatactcttt
    38701 gtctaagtaa tcagcgatta tgtgaaaggt aaatttgttt tgtttattat tttcaagaat
    38761 tgaagcaatt gaaatcgcag ccccaacctg gtagttctca tcaatacccc atgagacatt
    38821 aaacgtttcc cgttcatcaa gtttaacggg gcgcttgtcc agttcaatta tatctttaaa
    38881 ttgagtaaga tgtttaaaat ccaatttaat tacccttcat cataatcatt atttgctctt
    38941 caatcctagt ttgactttta catccctgat tactttttta attttctttt tgaatataag
    39001 atatgtatag ccaaagtctt tcaattcatt ttttatatta tagtgccgtt caagatcaat
    39061 gcgatctttt gcttttaaaa cagcggttgt ttttttacct gaaaggtcaa ttagtcttaa
    39121 acctttttcg gatactataa aatttccttt atgaggatcg ccagacacca ttccatgctg
    39181 atgcaactct tttatcgatt ctgataattg atccttcaga tcttcagaaa tttccaaata
    39241 ctcatttaac ccgacacctt caatatactc aatgagcata atataataat gagcgaaatt
    39301 taatgtttta cgttcagcta gcaaaaaata gtcattaatt gattggatgc cttcaccacg
    39361 tacccgatcg gtttgataaa ttaaattttc ataataatct ttttttatgc aagattttaa
    39421 gaaacgttcg gtcattttat gctttggcgc ataaacttta agtacaagag gtccacgagc
    39481 agtgtcaata agaatgactt tagtatcatc aatcgaacga aaaactttta atgttttaac
    39541 cctgcaagtt aaaaagtcat ttagaacttg ctcataaaag ggatcattat cttttatgaa
    39601 aactttcaac ccatttatcg ttttattata tatcataatg aattatttta accttaaatc
    39661 tttagaagca tttttcttta taatacttta aataattaaa aatgcctgcc atataatgct
    39721 tttgtttaaa tttatgtttt gcacaatacc gatactgatt gctatttaca ggtttaagta
    39781 aatcttcttt actccacgga gaagctgctt tggcgatcaa aaaactacgt gacaccggat
    39841 agtcagccca ctcatgccag ggttttgtag gtcccacata gtgtataaac acggtgtcat
    39901 cattgactgg attatcaact ttgtctttta attcatagtt aatactatat cgggtattat
    39961 atttctccga aataaacttc actttaccat tcaataatac attcagtaca tcttgatcaa
    40021 ggtgggtgat tttacttacc caatctgggt cacgcaacat ttcaatagct tttgacgaaa
    40081 tgttatttag attccactca tcaatattta tcagtaaaaa accagcattg aaatatccag
    40141 aagctaactg tggtgtagtt aataccgagg ctcgattttg ccaccattca acatctcttt
    40201 cagctacaac tgcggcaatt tcattagtag aaaattgata atctaagagt tctttaatac
    40261 tacccttgca agcaatatct gcatcaagat atagtatttt ttcatgttta tgatagaaat
    40321 aatctgcaat tataaatcga aaatatgttg cgtaagtcca gtttttcgtg cttggtaatg
    40381 acttcagctt atcacaattg ataacataaa tattaattct tgaattatat tgttttgcta
    40441 aatcagaaaa atataatttg tctttatcac tgatataatc tgtgaaaaca tgaaattcac
    40501 aagagatttc tctattgttt aatagaatag atgcgatggc tacaccacat ccaaaaagaa
    40561 aatttctgtc aataccaaat gctatatcaa gagtattttt agatcttata ttttgataat
    40621 gaaaattata ctcaaaaagg atgatgtcac tatcattgag ttgagacata tttattctgt
    40681 atccaaaatt ttggcaaaaa taagttacgg gttacaacga ttttcgaatc gttctttccc
    40741 tgatttttgt ggcttttgct tctgcttgcg acagcagtcc ttgttcctgc ttgagaatgt
    40801 ctttaagcgg ggcggcaaaa tacactttca taaaccgcca gatatcccgc tgagtcaggc
    40861 cgatattcat cgaagaaaaa taaagcccaa taagatcttt atcccgccaa cgacgtggaa
    40921 cgcgcgtgcg aagctgcgcc cggtgcaggt caattaccga aatttttaac tcctcttcct
    40981 taccggaaaa aggcaagtgc agcaggaaat gacagatata acagtcacgg tggttaatgc
    41041 ccgcagcatg catatcgcgc accatcgtcg cgacacgctt aataagcata cgctttacgc
    41101 gaacatctgg agggttagtc gcccagtcag cactgtaatc ttcaagactt atggttggtg
    41161 tcagatcttc ggtaataata aatgaagttc tggtcagcgg attcatgcct ttttcgccaa
    41221 atgccacccc atacatagta tcaacgccga catcccgcag tcgatgaatc gcattccatt
    41281 cgcggtcagc gcctaatact ggcatccgca atgagagtaa atttttgatt atctctttca
    41341 gggtcgtgcc gcgatgccat ttgagaaaat agcttttgcc cgccatttca aagcgcagag
    41401 tacggcgagt ttccagttca cgaaatacct caccctgcaa ggttttaact tcctcaaaag
    41461 gatctttgcc gcgccataac gtggcaaacg gctctttaag ttcaaccatc cagaccaccc
    41521 gttatgatat ccgccgcttt ctctggcaga ctgtataaat cttgtgtatc agcataatgt
    41581 cgcgcatttt ccgcccaggc ctggcgcaat gaagattgcg ttaacgcttt gcgtaaaatc
    41641 tcattcaatg tttcctggcg gaatggctca gcaatagcct cgccgcaatt agcgtcgaca
    41701 atataatgcg catagccaca aacggctgtt gttagtaccg gtaatcctgc agtaatcgct
    41761 tccagcagca caattcccgc cgcttcctgg taggcaggat gcagtaataa atccgccgcc
    41821 gccattaatt ccgagacatc attgcgcccc gagaaaaagt gaacattact gcgcacgccg
    41881 cgtttttctg ccagcgcctc aaattttcgc ggtttatcct gcccaacaac atatagcaat
    41941 gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt caatggaacg atcgacacct
    42001 ttacgcgtga agtctgaacc gacctgcaac aataaatatt gttgttcggt tattccattc
    42061 ttcttacgga agatttcacg gctattggct ggctgctggc tatatttacg atcaggataa
    42121 atccccggtg gcagaatatg aaaacgctcc gcttcagtct gataatgttt ctggaaatcg
    42181 gcgatttgct tatctgtcag catcagcaac tgtgtcggct taccctgttc gaaggttgcc
    42241 cgctcaaaga cggcataatg gcgataacgt gacgtcaggc gatagaaaaa gcctttttcc
    42301 tgcgctactt tctcggcata acaaacatca gcggcataat aaacgtccag ccccggcatt
    42361 ttgttgaatc caacgacttt atcgacggga tgttcgcgta aatgtttttg cacccaggca
    42421 aaatactccg cattgcgccc gtgattggta tgcgatttaa ccggcacttt gatcagttca
    42481 aatacatcag ggcattcgcc ttcccacgac tgggtataaa cccgaacatg atgacctcgg
    42541 gcggcgactg tctgagcaat acgcataaaa tcacgctgca aaccgccaaa gggaaaatat
    42601 ttatataaac aaaaagcaac gatcataacg acgtacctgt cgtggaggag ggcagtaatt
    42661 tatcgacagc agcaatgaca tctgccgccg gaataaccga aagatacatc tcatttcggt
    42721 cacgctgatc gcgcgttggc atttcccggt aatctcccgc ccagaattga atcatgttat
    42781 ttgaccaggg acgccagaaa atatgatctg tcgcaccaaa cagcgatatc agcggcgtat
    42841 taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc aataaacagc tgcgcatgat
    42901 cgattaacgc accaagttcc gggaaggtca cctttccagc cagcgccgtt actggtggcg
    42961 tctggcatcc ctgcgcaatt tcattgacgc aggccagatc gtctttatct gggccggacg
    43021 tcagaacgac ttcataacca cgagcatgta aggcatcaat cacagcggaa aacttggcgt
    43081 tgtcccagca tttgaagatt tgccgcgccg taggttggat aaccacataa ttttgtccaa
    43141 caccagcgtg atcaagttcg cgacgcatac gtttccagct tgcaggcggg taactcatgg
    43201 ttgtctgctt caccaacgaa tcaagtccca atggtgtcag cacggataag ttactttcca
    43261 ccacatttcc accctgcaac ggcaccaaat gggtgaaact tttacgccaa aaagcagact
    43321 gccgatgatg ataatcctgg gaaattttca cacgagcatt taataagcga accagtatag
    43381 caaccatcca ttgatcggta agattgacga taaggtcata cttattggca cgtaatacct
    43441 tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt tgctttttta ttttttatgc
    43501 cgtagagcgc gttaatctct ggattttcag acaggatcgg gatggtgtcc tgataaagca
    43561 gcacatcgat ttttgcgtca gggtaatttt ttttcagcga actaatgacg ggagtagtta
    43621 ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat tcttcgaaat ggcttatcca
    43681 caagtaactc tttttgttga gattatccga ttaaggtgaa gcagaaaaag cacgctaccg
    43741 ccccaggctc aacagctacc tgaatactga taccgcactg atattgacgc gctagtgtat
    43801 cacttctttc gcggcaatcc gacccgaatg atttttaagc acattgttga ttttcaataa
    43861 agaaatcaat caaataaaat cattagcaac catattcttt cactgttgct ctgcgattcc
    43921 ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa tacaaaaaaa gtatagatgt
    43981 tcagcaagaa ttggcgggta aattgctatt taagtcaaaa acgggaaaag taatggtaaa
    44041 gccacagcta aatacataga atccccagca catccataag tcagctattt actatgctcg
    44101 aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata cggctctggg
    44161 tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac ggtttttacc
    44221 gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt gaaactctgg
    44281 cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg attaccgtta
    44341 caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag gatgttcagc
    44401 acgtttatct gccgtatgac ctgcccgatg cactcaaccg tttcctgaat aaagtcgacc
    44461 ctaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg gcgctacata
    44521 aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg gccgcaggtt
    44581 atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg attgctgcgc
    44641 aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac caggtaaccg
    44701 ttaccggtag cctgaaattc gatatttctg taacgccgca gttggctgct aaagccgtga
    44761 cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc actcacgaag
    44821 gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc ccgaatttat
    44881 tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac cttgtccgcc
    44941 aggctggact aagctatatc acacgctctt caggggaagt cccctccacc agcacgcagg
    45001 ttgtagttgg cgatacgatg ggcgagttga tgttactgta cggcattgcc gatctcgcct
    45061 ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct gccgcacacg
    45121 ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc gcgcggctgg
    45181 agcaggcaag cgggctgatt accgttaccg atgccactac gcttgcaaaa gaggtgtcct
    45241 ctttactcac cgacgccgat taccgtagtt tctatggccg tcatgccgtt gaagtactgt
    45301 atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac ctgccaccga
    45361 aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt cgatcccatt
    45421 accaatggtc atatcgatat cgtgacgcgc gccacgcaaa tgttcgatca cgttattctg
    45481 gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg tgtggcgctg
    45541 gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag tgatttgatg
    45601 gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg tgcggtggcg
    45661 gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc ggaactggaa
    45721 agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt ggtgaaagag
    45781 gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca tcaggcgctg
    45841 atggcgaagt tagcgtagcg tttatgccgg atggtatgcc atccggcgcg catgaattac
    45901 ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc cacaatcggc
    45961 gtaccgcaca cccgacacgg ctcgcctttt cgcccgtaaa cctgcaattc ctgagcgaaa
    46021 tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc ctgctcaatc
    46081 gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc cagcgacagt
    46141 gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct ggcatagata
    46201 ttccctaccc ctaccaccag cttgttatcc atcagccacg gtttaatcgc cgtttttttc
    46261 ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg ctccggtcca
    46321 agatgggtca gcacattatg cccttccagc tctttggtcc acagccaggc accaaagcgg
    46381 cgcggatcgg tgtagcgcag cactttgccg ttgctcatca ccaattccac atggtcatgc
    46441 ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc taaatgaatg
    46501 ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg cacgctaagc
    46561 actggttggt cgcttaaacg gtagatctct tctgaaaccg gccagcgcaa gcgtccgttg
    46621 cgcaccactg catgaagaat ggttgcacca acgagatgcg gttctatgcc gcggcggctg
    46681 gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg cagtcaatat
    46741 gggggcaagc agataacaaa aaaccccgcc ggagcgaggt tttttgttac atcaaagcga
    46801 gaattatttg attttcgctt ctttgtagat cacgtgctgg cgaacaactg gatcgaattt
    46861 tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat agaagtgacc
    46921 agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca tgatttattt
    46981 cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac tgtatcgatg
    47041 ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac aaaacgcttc
    47101 tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg tttagtcgcg
    47161 ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac ttggcagact
    47221 cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg gtattggcgc
    47281 ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat tgccaggccc
    47341 aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag cgatgtgctc
    47401 aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat ccagccgcgt
    47461 tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac gcgtaaatcc
    47521 atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt actgggttca
    47581 gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat ttttatcgct
    47641 tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga aaaaagacgg
    47701 ctatgcgtta taacccggtg ttgggagtcg agaaagatca ccataaagat ctcccgctcc
    47761 tcacccgtga gctggctttg taaaaattcc ctcgtcatct ccgggctgag taaagggctt
    47821 tcttcacgca tccgcacgtt gtagtaacgc cgcgccagtt cagcaatccc ctttaactgg
    47881 gcaaatttcg ccacgccaat tccatgaacg ccactaaatt gttcatattc agaggttaac
    47941 aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa tacatcttta
    48001 ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa ggcgctaata
    48061 ccaaacttca gcattttttc gcgcggcatc aacagctgtg aattgttttt caccttcacc
    48121 tcctttgtgg tgctcgcatc ctgtcatagc cgtttcgtta aatcgacggc cagttttcaa
    48181 tcctggaaag cgcctcgcaa agtgaatctc aggtgaaggc acgacaacaa aagtattgtg
    48241 ataaaatcgc caacttctgg tgtcacccta caggaaaaat catcatgagc ctggccggta
    48301 aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct gaactggtgc
    48361 gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg gcaaaagcct
    48421 ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac agtctgctgg
    48481 acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct gatttagtga
    48541 ttctcgcccc tgccacggca gatttgattg cccgtattgc tgccggaatg gcgaatgacc
    48601 tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc gccatgaacc
    48661 agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct tcccgtggta
    48721 tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt cctgggcgaa
    48781 tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc gtcaacgacc
    48841 tgaaacatct gaacattatg attaccgccg gcccgacgcg tgaaccgctc gatccggtgc
    48901 gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc gccgctgccc
    48961 gtcgtggcgc gaacgtcacg ctggtatcag gtccggtttc actaccgacg ccaccgtttg
    49021 ttaaacgtgt tgatgtgatg actgcgctgg aaatggaagc cgccgtgaat gcttctgtac
    49081 agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca gctaccgtgg
    49141 ccccagagaa aatcaaaaag caggccacgc agggtgatga attaacaata aaaatggtta
    49201 aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc tacgtcgttg
    49261 gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt atccgtaaaa
    49321 accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt aacagcgaca
    49381 acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt gagcgcaaag
    49441 agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa aaaaatcgac
    49501 gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta tgccacctct
    49561 ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact ggctccgggt
    49621 gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc actggcggca
    49681 atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg taacctggta
    49741 ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa ccgtggtcag
    49801 gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt tccggtagta
    49861 caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga aggcggcttt
    49921 ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca tagccgcaaa
    49981 catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt caggggtatt
    50041 ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa tacttcagtc
    50101 tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg caaaactggc
    50161 cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta agacccgcat
    50221 gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca atctgattct
    50281 gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc ttctcggttt
    50341 tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa tgtttgaaca
    50401 ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc agctgcgtca
    50461 ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg aaaccctgct
    50521 ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc gcagcgaatt
    50581 taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgcgg cacagttgca
    50641 gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca aactcttcgc
    50701 gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc aggtaagcaa
    50761 tcaggtcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc tcaacttcct
    50821 gaatcgccga aatctcgccg cgcccccgtt cctgacgatc aagcgaaatc aacacgccag
    50881 caagcgtcgc gccattagcc tgaataatct ccatcgattc gcgaatcgcc gttccggcgg
    50941 tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc agattgccgc
    51001 cttcaccgtg gtcttttgcc tctttgcggt taaagcagta cggcaggtca aggtcatgat
    51061 gctccgccag cgccacggcg gtagtggtgg caatcgggat ccctttgtaa gcagggccaa
    51121 acagcagatc gaactcaagg ccggaatcca ccaacgcttc agcgtaaaaa cggcctaaca
    51181 gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg cttttgcgcc
    51241 cggacttcaa cgtaaactcg ccaaacttta acacctgctt gccaagcgca aattcaataa
    51301 actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac agacaaaaaa
    51361 aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct gcgtcgctac
    51421 aatggattcg attccccctc gggccagagc caacaaggtg agtagctctt catgggtgaa
    51481 cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg tcatcactac
    51541 gttcatgtcg gtctctgcgg cagagtcttc aacgtattcc agatcgcaaa ccgcttcgcc
    51601 gttcacaatt ccgacagaaa ctgcggctac catccctttc atcggattgg ttttcagctt
    51661 gccgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac ccgtaatcga
    51721 cgcggtacgc gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa tggtgaactc
    51781 acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg gcgatcagac gctggatttc
    51841 cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg tgtgggtaga
    51901 acgtggcagc atgccgtact ctgcggtgat ccagccctgg ccctgacctt tcaggaagcg
    51961 cggcacgcct tcttcaatag aggcggtaca caacactttg gtatcgccaa attcgaccag
    52021 caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac gcacctgatt
    52081 attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat gtggctgcgc
    52141 attatacgga cttccggcgg ttattcctat cctgacaagg catcgatggc tataatcctt
    52201 ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc ctacgcccgg
    52261 cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt aaaccagcgt
    52321 tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc tgtcgttcgc
    52381 gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg ctatgagcca
    52441 gatgttagcg cgcaaggtga gctgatcctc aacgaaaaac tggctaaaca gctggtaact
    52501 gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa tcaacccggt tgatattcta
    52561 cgctggccgg gcgtgatggc agcccaggag caggatcttg acgccattgc cgctgaaatt
    52621 ctcgcggcgc tggatggtac gctggacgac tttattgtcg cgcgcgaaac cgaaggtcag
    52681 gcactgaaag cattgatcga gcagcgtctg gaaggcgtca ccgccgaagt ggtcaaagtc
    52741 cgcgcccata tgccggaaat cctgcaatgg cagcgtgagc gtctggtcgc gaagctggaa
    52801 gatgctcagg ttcagttgga aaacaaccgt ctggagcagg agctggttct gctggcacaa
    52861 cgaattgacg ttgccgaaga actggaccgc ctggaagcgc atgtcaaaga gacctacaac
    52921 attctgaaga aaaaagaagc cgttggtcgt cgtctggact ttatgatgca ggaattcaac
    52981 cgcgagtcga acactcttgc gtcgaagtct atcaatgccg aagtgacaaa ctccgcgatt
    53041 gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatatcga ataagctatc
    53101 agcaaaaatc accaagcagc aaaaaaccat aaagcccgcg aaatgtcggg cttttttgtt
    53161 atcatgagct atcataacga cgcaacaaag cgcacaatat gcgtgtacat agaggtgtac
    53221 ataatgattt tcatgtacat tgctttatgt acacacccat attcgatatt caggtattcg
    53281 caatgccaaa actcacagat atgcagatcc gcgcatggat aaaggcggga gacagatttg
    53341 atggcaaggc agacggcaac ggcctttata tttgctaccc caaaaactac acagtcccgt
    53401 tctggcgatt ccgatataag ctcgcaggaa aacagcgggc tatggttatc ggctcttact
    53461 ccgaactgtc actatcaaaa gccagagaaa cagcaaaaga gctgtcagcc agagttgctc
    53521 ttggctatga cgtagcagca gaaaaacagg aacgaaaagc tgaagcactg gcaaaaatgg
    53581 aggctgagaa aaacgccatg cgcgtttcag atctggccgc cgaatacttc gagcgacaga
    53641 tacttccgag atggaaacat cccgatatcc ttcgccggag aattgataag gatattaacc
    53701 cctgcattgg gcatatgaag gtagaggacg ttaagccacg ccacattgac gacatgttga
    53761 aaggtatcgt tgaccgagga gcaccaacga tagcaacaga tgtattacgc tggacacgcc
    53821 gtatctttga ctatgggata aaacgccacg ctctggagat aaacccttgt tcagcgtttg
    53881 aagtatctga tgctggtggt aaagaggttt cccgcgaccg ttggctaact cgcgatgagc
    53941 ttatcaggct attcgaggct atgcgcaccg caaaaggatt cagccgccag aacgaactga
    54001 catttaagct attgctggca ctatgcgttc gtaagatgga gttatgcgcc gcacgatggg
    54061 aagagtttga tttagatggt gcggtatggc atttgccgga agaacgcagc aaaaacggag
    54121 accctattga tatacctcta ccttctcctg ccgttgaatg gttgagagag ctacacacct
    54181 tttcatgtaa tagcgcatgg gtgcttccag ctaggaagat gcaaaacaga atgatcccac
    54241 atattcagga aagcacttta cccgtagcac tggctaaggt tcgcgccgaa atgccggatg
    54301 tgcctaattt cacgattcac gacttccggc gtaccgcacg tactcattta gcagcattgg
    54361 gtgttgatcc tgtcgtagcg gaacgatgcc tcaatcatcg cattaagggt gtggaggggg
    54421 tttataaccg ccatcagtat tttgatgagc gtaaagctgc gctggcacag tgggctgatc
    54481 tgctggtggc actggaaagc gggaaagact acaacgtaac gccattcgat aaagcccgga
    54541 ggcaaatgtg aacaacatct tgagaaaatc gccaatcgaa cggctttgtt catctgtatc
    54601 gattacccct catgaaatgg ctgtggcctt ggctggccta aacccatcta tgcgcattag
    54661 cgatgtgcct gaggaaaatt tcgaatatgt cgattttgtc agaactcatc ttgccagagc
    54721 tatcaaggtg tacagaggtg aaaaaactag taaagacgag ccttgccatg cccttgatat
    54781 tttcttagct tcttacccat ttattgacac aaatacacca gaaataattg ttcagaaaat
    54841 atcagaagcg attgatgatt tgcgtggcac caagggctgg gaagaaaaag cccgaaacct
    54901 tggaggactc cagttagtaa actacatcaa agaaaccaac cgcagcggac gtgggcagca
    54961 tagaaagcag gatgaggaaa acgggacaat gaaaatgatg gggttgatag tacatctgct
    55021 gataaaaaaa tcaggctcat cttcctacat ccaaaatggg aaacctaatc gcacagctat
    55081 ataccgtgac atagaaacct taatcaagga tgaagggata tcccataaag gaatagcaaa
    55141 agccacattc ctagaaaaaa tctcacgggc tttattagct gtccacgatc tcgactaatc
    55201 acttttcacg cccctccaaa gcaatggcgg ggcattacct gtttacccat ctccaccagc
    55261 atagataatt ctatgcctag ccattgctat atctgttcgg cttagttcat ctgtaatcac
    55321 cccatctcta aaagatacgc cagctatcat tagccatttt aagccatcat gagccattat
    55381 aaaccgactc aaccaattga ataataaaag ataaaaggaa atgtaaaaac atgatgaatg
    55441 acgatgaaag atataactct ttgataaaac aatgattttt taaactggta actaattgaa
    55501 aataaagaaa ataattatcc cacctgcatt tgcaatcttt cagtggatcg gtattttttc
    55561 aatagattta taatttcttt caatagattc tagtcttaat agaaatactg tatgggcaac
    55621 cactaaactt taagtaactt catgagctat cagagaggtt actagagata tgacagcaca
    55681 caagccagca tcatcagcta tcccaacaac tggatacatc cgtcgctttc gcatgccgga
    55741 attgctaggt gtttcactgc ctactattga tagatgggtt aaaaatggca ctcttccacg
    55801 cccagtgaag ctaacagata acgtgactgc gtttgatgcg gtggaaatta ataactggct
    55861 ggcagaacgc cgaaacacat cagcctgatt aaatgactac gggggatgac gttcaccccc
    55921 accagcaaac aaaagtaact taatttaata gcaggtgttt ttatgaaact gcgaaaaacg
    55981 cccgtacagg ggcagggctt cgttcggcct gaaaccagca tgagcgatat tctggttcgc
    56041 cacaaagcgg agtcgcgcat tgattcccgc ttatttgctg agcgtattgg cataaagcac
    56101 aaaaaccttt attcgctcat tcagaagcac aaaaccggat tgcgtgaact gggcatcctt
    56161 ccgtttcaaa cggagcgatt aaaaacccgt caaaacgggg agcgtgaaca taagtttgcc
    56221 ctcctgaata gcgatcagtt tgattacatg tgtcgcattg tgcgcggtcg tgatgatgaa
    56281 cgcatgaacc gcttcaagct ggatgttacg aaggcattcg caaaacatcg cgctaccgag
    56341 cctgtacgcc gtgaatatct gccaggctat cacgaaagcc gtgacgggct taaagcactg
    56401 ggcgcagaac gtcaccatta catcaatctg gcacgcgcag agaaccgtgt tacaggtctg
    56461 gtaactggcg aacgaggcac agccagcgag cagcagttag gcttgctgat tgtcatgcag
    56521 aaaatcgaac aggccgcatt tgaggaggcg atacagaacg gcatgacacc taccgaagca
    56581 gtgcgggagg ttgcccgacg catggagaca ttcgccagcc tgatgacatc tgagccgtat
    56641 ctgggggtaa atcatgctta aggatttcgc cgcaattatt ccggtatcac ctggcattat
    56701 tggcggacat gaaacagcca ttgtcagcgc cagagcgtta cataaggcgc ttggtgtgaa
    56761 gcgtgatttc actacatgga ttaaagggcg tattgatcag tacggattta aaaatggcgt
    56821 ggattttgag tttgtagaaa atttgacctc ccccaatccg gtgagcgcaa aatctcgcca
    56881 acgtgtagag cacgactacc tgagtaccat caacatggcg aaagaacttg cgatggtcga
    56941 gcgtaccgaa cagggccgcg ctattcgtca gtacttcatc aaatgcgagg aggagctaca
    57001 caaagtagcg ccagtgcgtt ccgcagcgtt acgccgtgaa ctgaaagccc gtatcaccgt
    57061 tgccagctac tttaagccga tgtgtgccgc gctggaggcg taccgcgctg aactgggtaa
    57121 aaacacgctc cagcaccact acaccacgga agccaatatg ctggcgcgta tcgtgctggg
    57181 tggcatgaat gcaaaacagt gggcgcaggc gaacggcatc acaggcgaac cacgcgacca
    57241 catgagcacg ctgcagcttg agcacctttc ttaccttgag cagagcaata tcacgctgat
    57301 tgagttaggc caggactacc accagcggaa agctgaatta attcgtcttt cgcaacgctg
    57361 gttagcccgc cgcatggagg tgagcagcca tgcgtaaacc tgtcttttat gccggtcgca
    57421 ctgaaatctc tgtcattaaa ttcgatgaca ttcaggtgcg catagtgagt atcaacggcg
    57481 agccgtggtt tgttggcgct gatgtgtgtg ccgcgctgga aatcagcaat gttactgatg
    57541 ccgtttcagt tctggataac gatgaggtaa tgaccctagc tttaaccgag ggtcattccg
    57601 gtaagcgtgg tggtgccaga agctggaatg ttgtctctga atcaggtttc tacaaactga
    57661 tcgcccgcag ccgtaaagct tccacaccag gcacatttgc gcaccgtttc agtaactggg
    57721 tattccgcga ggtgattcct tcaatccgca aaacgggggg ttatggtgtt ccgtttgcca
    57781 tgctgaatga tttcagtaaa cgtcaggctg catatcaaca ggaagccagc aaacgcggtt
    57841 acaggttaca gcaatgcaaa gaggcaaaag atgcactgga acgagaagag cgggcgctat
    57901 ggcgtaaata tcagcctgat tttctggagg gtgaaagcaa tgtgtaacga tccgaacgtt
    57961 acagaaagaa aaagcgcccc gttgctggat aacaggcaaa acaaaggcga ccgggatagg
    58021 tcgccagtgg gaacaaagga aatcaaacgc atcgccaaca atgccagatt tgcggctggt
    58081 gggcaatgtg atcagtgtgc tggcttgctg gtgggctatt cctgctcttt agcatcgcca
    58141 tactgcgatg ttttccaggt atgcgat
//
LOCUS       DAHBHC010000022        56684 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHBHC010000022 DAHBHC010000000
VERSION     DAHBHC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.022"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            42..118
                     /locus_tag="M2A35_001740"
     tRNA            42..118
                     /locus_tag="M2A35_001740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:tgg)
     gene            265..1500
                     /locus_tag="M2A35_001741"
     CDS             265..1500
                     /locus_tag="M2A35_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCB7198054.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSLQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1584..1745)
                     /locus_tag="M2A35_001742"
     CDS             complement(1584..1745)
                     /locus_tag="M2A35_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198055.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(2423..3619)
                     /gene="hemY"
                     /locus_tag="M2A35_001743"
     CDS             complement(2423..3619)
                     /gene="hemY"
                     /locus_tag="M2A35_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCB7198056.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3622..4833)
                     /gene="hemX"
                     /locus_tag="M2A35_001744"
     CDS             complement(3622..4833)
                     /gene="hemX"
                     /locus_tag="M2A35_001744"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCB7198057.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFIAIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(4855..5595)
                     /gene="hemD"
                     /locus_tag="M2A35_001745"
     CDS             complement(4855..5595)
                     /gene="hemD"
                     /locus_tag="M2A35_001745"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCB7198058.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(5592..6533)
                     /gene="hemC"
                     /locus_tag="M2A35_001746"
     CDS             complement(5592..6533)
                     /gene="hemC"
                     /locus_tag="M2A35_001746"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCB7198059.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6920..9466
                     /gene="cyaA"
                     /locus_tag="M2A35_001747"
     CDS             6920..9466
                     /gene="cyaA"
                     /locus_tag="M2A35_001747"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCB7198060.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(9506..9826)
                     /gene="cyaY"
                     /locus_tag="M2A35_001748"
     CDS             complement(9506..9826)
                     /gene="cyaY"
                     /locus_tag="M2A35_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCB7198061.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9874..11199)
                     /locus_tag="M2A35_001749"
     CDS             complement(9874..11199)
                     /locus_tag="M2A35_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCB7198062.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(11196..12674)
                     /locus_tag="M2A35_001750"
     CDS             complement(11196..12674)
                     /locus_tag="M2A35_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCB7198063.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKRTDNNGGGNRL
                     DIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQKSWDQRGKFST
                     AFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(13042..13596)
                     /locus_tag="M2A35_001751"
     CDS             complement(13042..13596)
                     /locus_tag="M2A35_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HCB7198064.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            13946..14149
                     /locus_tag="M2A35_001752"
     CDS             13946..14149
                     /locus_tag="M2A35_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7198065.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            14186..15010
                     /gene="dapF"
                     /locus_tag="M2A35_001753"
     CDS             14186..15010
                     /gene="dapF"
                     /locus_tag="M2A35_001753"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCB7198066.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            15007..15714
                     /locus_tag="M2A35_001754"
     CDS             15007..15714
                     /locus_tag="M2A35_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCB7198067.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            15711..16607
                     /gene="xerC"
                     /locus_tag="M2A35_001755"
     CDS             15711..16607
                     /gene="xerC"
                     /locus_tag="M2A35_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCB7198068.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            16607..17323
                     /gene="yigB"
                     /locus_tag="M2A35_001756"
     CDS             16607..17323
                     /gene="yigB"
                     /locus_tag="M2A35_001756"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCB7198069.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            17407..19569
                     /gene="uvrD"
                     /locus_tag="M2A35_001757"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             17407..19569
                     /gene="uvrD"
                     /locus_tag="M2A35_001757"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCB7198070.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(19716..20480)
                     /locus_tag="M2A35_001758"
     CDS             complement(19716..20480)
                     /locus_tag="M2A35_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCB7198071.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            20850..21800
                     /gene="corA"
                     /locus_tag="M2A35_001759"
     CDS             20850..21800
                     /gene="corA"
                     /locus_tag="M2A35_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCB7198072.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(21842..22333)
                     /locus_tag="M2A35_001760"
     CDS             complement(21842..22333)
                     /locus_tag="M2A35_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCB7198073.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(22330..23178)
                     /locus_tag="M2A35_001761"
     CDS             complement(22330..23178)
                     /locus_tag="M2A35_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCB7198074.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQNYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            23335..23511
                     /locus_tag="M2A35_001762"
     CDS             23335..23511
                     /locus_tag="M2A35_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCB7198075.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGAIIFMILA
                     GLTSYLYFKRKNWL"
     gene            24133..24432
                     /locus_tag="M2A35_001763"
     CDS             24133..24432
                     /locus_tag="M2A35_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198076.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILALFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFIKR"
     gene            complement(24479..25366)
                     /gene="rarD"
                     /locus_tag="M2A35_001764"
     CDS             complement(24479..25366)
                     /gene="rarD"
                     /locus_tag="M2A35_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCB7198077.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(25418..25885)
                     /locus_tag="M2A35_001765"
     CDS             complement(25418..25885)
                     /locus_tag="M2A35_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCB7198078.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            26050..26919
                     /gene="pldA"
                     /locus_tag="M2A35_001766"
     CDS             26050..26919
                     /gene="pldA"
                     /locus_tag="M2A35_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCB7198079.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            27052..28881
                     /gene="recQ"
                     /locus_tag="M2A35_001767"
     CDS             27052..28881
                     /gene="recQ"
                     /locus_tag="M2A35_001767"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCB7198080.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            28945..29565
                     /gene="rhtC"
                     /locus_tag="M2A35_001768"
     CDS             28945..29565
                     /gene="rhtC"
                     /locus_tag="M2A35_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCB7198081.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(29627..30247)
                     /gene="rhtB"
                     /locus_tag="M2A35_001769"
     CDS             complement(29627..30247)
                     /gene="rhtB"
                     /locus_tag="M2A35_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCB7198082.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            30358..31380
                     /gene="pldB"
                     /locus_tag="M2A35_001770"
     CDS             30358..31380
                     /gene="pldB"
                     /locus_tag="M2A35_001770"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCB7198083.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            31388..32188
                     /gene="yigL"
                     /locus_tag="M2A35_001771"
     CDS             31388..32188
                     /gene="yigL"
                     /locus_tag="M2A35_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCB7198084.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            32264..33163
                     /gene="bioP"
                     /locus_tag="M2A35_001772"
     CDS             32264..33163
                     /gene="bioP"
                     /locus_tag="M2A35_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCB7198085.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(33051..34004)
                     /gene="metR"
                     /locus_tag="M2A35_001773"
     CDS             complement(33051..34004)
                     /gene="metR"
                     /locus_tag="M2A35_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCB7198086.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            34127..36388
                     /gene="metE"
                     /locus_tag="M2A35_001774"
     CDS             34127..36388
                     /gene="metE"
                     /locus_tag="M2A35_001774"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCB7198087.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(36427..37242)
                     /locus_tag="M2A35_001775"
     CDS             complement(36427..37242)
                     /locus_tag="M2A35_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCB7198088.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            37504..38265
                     /gene="udp"
                     /locus_tag="M2A35_001776"
     CDS             37504..38265
                     /gene="udp"
                     /locus_tag="M2A35_001776"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCB7198089.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            38406..39833
                     /gene="rmuC"
                     /locus_tag="M2A35_001777"
     CDS             38406..39833
                     /gene="rmuC"
                     /locus_tag="M2A35_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCB7198090.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            39928..40683
                     /gene="ubiE"
                     /locus_tag="M2A35_001778"
     CDS             39928..40683
                     /gene="ubiE"
                     /locus_tag="M2A35_001778"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCB7198091.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            40697..41302
                     /gene="ubiJ"
                     /locus_tag="M2A35_001779"
     CDS             40697..41302
                     /gene="ubiJ"
                     /locus_tag="M2A35_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCB7198092.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            41299..42939
                     /gene="ubiB"
                     /locus_tag="M2A35_001780"
     CDS             41299..42939
                     /gene="ubiB"
                     /locus_tag="M2A35_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCB7198093.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            43018..43287
                     /gene="tatA"
                     /locus_tag="M2A35_001781"
     CDS             43018..43287
                     /gene="tatA"
                     /locus_tag="M2A35_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCB7198094.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            43291..43806
                     /gene="tatB"
                     /locus_tag="M2A35_001782"
     CDS             43291..43806
                     /gene="tatB"
                     /locus_tag="M2A35_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCB7198095.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            43809..44585
                     /gene="tatC"
                     /locus_tag="M2A35_001783"
     CDS             43809..44585
                     /gene="tatC"
                     /locus_tag="M2A35_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCB7198096.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            44627..45409
                     /gene="tatD"
                     /locus_tag="M2A35_001784"
     CDS             44627..45409
                     /gene="tatD"
                     /locus_tag="M2A35_001784"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCB7198097.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(45406..45894)
                     /gene="rfaH"
                     /locus_tag="M2A35_001785"
     CDS             complement(45406..45894)
                     /gene="rfaH"
                     /locus_tag="M2A35_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCB7198098.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            46061..47554
                     /gene="ubiD"
                     /locus_tag="M2A35_001786"
     CDS             46061..47554
                     /gene="ubiD"
                     /locus_tag="M2A35_001786"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCB7198099.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            47600..48301
                     /gene="fre"
                     /locus_tag="M2A35_001787"
     CDS             47600..48301
                     /gene="fre"
                     /locus_tag="M2A35_001787"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCB7198100.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(48493..49656)
                     /gene="fadA"
                     /locus_tag="M2A35_001788"
     CDS             complement(48493..49656)
                     /gene="fadA"
                     /locus_tag="M2A35_001788"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCB7198101.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(49666..51855)
                     /gene="fadB"
                     /locus_tag="M2A35_001789"
     CDS             complement(49666..51855)
                     /gene="fadB"
                     /locus_tag="M2A35_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCB7198102.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            52045..53376
                     /gene="pepQ"
                     /locus_tag="M2A35_001790"
     CDS             52045..53376
                     /gene="pepQ"
                     /locus_tag="M2A35_001790"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCB7198103.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            53376..53990
                     /locus_tag="M2A35_001791"
     CDS             53376..53990
                     /locus_tag="M2A35_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCB7198104.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLVDFSRGSLQLLAIEE"
     gene            54029..55480
                     /gene="trkH"
                     /locus_tag="M2A35_001792"
     CDS             54029..55480
                     /gene="trkH"
                     /locus_tag="M2A35_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCB7198105.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            55492..56037
                     /gene="hemG"
                     /locus_tag="M2A35_001793"
     CDS             55492..56037
                     /gene="hemG"
                     /locus_tag="M2A35_001793"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCB7198106.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            56413..>56684
                     /locus_tag="M2A35_001794"
     rRNA            56413..>56684
                     /locus_tag="M2A35_001794"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13434 a        14195 c        15027 g        14028 t
ORIGIN      
        1 gactttaaag aattgaacta aaaattcaaa aagcagtatt tcggcgagta gcgcagcttg
       61 gtagcgcaac tggtttggga ccagtgggtc ggaggttcga atcctctctc gccgaccaat
      121 tttgaacccc gcttcggcgg ggttttttgt tttctgtgca tttcgtcacc ctcccttcgc
      181 aataaacgcc cgtaataact cattgcccca cggtatgatt tcgcccttaa cgtattgaag
      241 gatgacttca ggcaaggagt gaccatgctg caacaggttc caacgcgtgc ttttcatgtg
      301 atggcgaaac cgagtggttc cgattgtaat ctgaactgtg actactgttt ttatctcgaa
      361 aaacaatccc tttaccgcga aaagccagtc acgcatatgg acgatgacac gctggaagcg
      421 tatgtccgtc actatatcgc tgccagcgaa ccgcaaaacg aagtggcttt tacctggcag
      481 ggcggcgaac caacgttact cgggctggat ttttaccgcc gtgccgtaaa gttacaggcg
      541 aaatacggtg ctggcaggaa gataagtaac agcttccaga ctaacggcgt gctgctcgat
      601 gataaatggt gtgcatttct ggcagaaaat cattttcttg ttgggttatc gctggacggt
      661 ccggctgaga tccacaatca atatcgcgtg accaaaggtg gcagaccaac gcataagctg
      721 gtgatgcgtg ccctgacgct gctgcaaaaa catcatgtcg actataacgt gctggtctgc
      781 gtcaaccgca ccagcgcgca gcaaccgttg caggtttatg attttttgtg cgatgcggga
      841 gtcgaattca tccagtttat tccggtggtc gagcgcctgg ctgatgaaac agctgccagc
      901 gatggactga aactacatgc gcctggtgat attcaggggg aactgacgga atggtcggtg
      961 cgccccgatg aatttggtga atttctggtg gcgatattcg accactggat caaacgcgac
     1021 gtcggcaaga ttttcgtgat gaatatcgaa tgggcgtttg ccaattttgt cggtgcgccg
     1081 ggtgcggttt gccatcatca gccaacctgt gggcgctcgg tgattgttga gcacaacggc
     1141 gacgtttacg cctgcgatca ctatgtttat ccgcaatatc ggctggggaa tatgcatcag
     1201 caaacaattg cagaaatgat cgattccctg caacagcagg tgtttggtga agataaattt
     1261 aagcaattac cggcgcagtg tcgcagttgt aacgtgttaa aagcgtgctg gggaggctgc
     1321 ccgaaacacc gcttcatgct cgatgccagc ggcaaaccgg ggctgaatta tttgtgtgcc
     1381 gggtatcagc gttatttccg ccatctaccg ccatatctta aagcaatggc tgatttgctg
     1441 gcgcacggtc gcccggccag tgacattatg caggcacatt tgctggtggt gaataagtaa
     1501 aaaagcggcc attttgtggc cgcctgcggt tgattgccgg atgcggcgta aacgccttat
     1561 ccggcctaca tgatcgtgca aattcaataa attgcagcgt tctgtggata ctggtttggg
     1621 tgatcgtatc cggcaaagcc tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg
     1681 gccatgagag gaagagcggc ggcgacagca acaacaagaa gtttgggtga aaacgaaaat
     1741 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
     1801 ttgagatccc tctccctgac aggatgatta cataaataat agtgataaaa ataaattatt
     1861 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
     1921 agttgatatg taaggcaggt ttattatatc acgttgatta ttgatgcttt tttagtttta
     1981 acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa taagtatgtg
     2041 taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa gcaaagagga
     2101 ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct ctgtggcata
     2161 ataaacgggt agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg
     2221 aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat
     2281 cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt
     2341 aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt cccggaggtg
     2401 cttgcctccg ggtgagaagg aactactgtg gtgggttgtt ctgcaacgtt aacatcaaac
     2461 cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa
     2521 gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg
     2581 cttcctgcca ttctccgtgc ttcatcagtg actggcccag tgtgctccac aacagcgggc
     2641 gatcgccgac gtttttgatc tgctggcgca gcactttttc cagctgttcc ggattgttgg
     2701 ttttcagtcg aggaatcggc agcagtaggc gatcgtcgta ctggcgtttc aggccatcga
     2761 tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca
     2821 cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac
     2881 cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca
     2941 gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca
     3001 gcagagaact ccatgcacct gtgcggatat acgcctgttc cgccagacgt aatacttccg
     3061 gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca tggttttcat
     3121 tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca
     3181 gctctgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg
     3241 cttcggcagc cagtagatag ttcaccaccg gttgttccgc gtgatcggca tttttcgcca
     3301 tcagcttttc aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct
     3361 gcttacgggc acggcgacgc ttacgtccga caaaccaccc acgggtgtgt gcgcctgtgc
     3421 ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa
     3481 taatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcagtctgg atcagcacgt
     3541 aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga
     3601 gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcaccttg
     3661 cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt
     3721 ccccgctgcc ggttgtgcca gcaggttacg cacgcgggtc tgcatcagtt tttccagcat
     3781 cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc
     3841 cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag ctcgtaccca
     3901 ggtggagacg ttctccagcg cctggcgata agtctcttcc tggtgacgcg gtacagcttg
     3961 tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc
     4021 taacagcggt acggcggtgt catcacgacg gcgaatcgca atgaagttgt ccataaagtt
     4081 ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc
     4141 gtcggaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg
     4201 atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg aaactgcaga
     4261 aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat
     4321 atccgccagg ctggcatctg cacttttcag caacgctgca gcggtcgtga cgtcctgatc
     4381 gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca gcagccaggt
     4441 tttagcatcg ctgccggaaa tggtggcgac cttttgttgg acttcatcca actgttttgc
     4501 cagcgtttct tgctgacgat tcgcctgctt aagctgtgca gcttgttgct taataatgcc
     4561 ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag
     4621 ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac
     4681 acccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcag tattgttctt
     4741 actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc tggtctcttc
     4801 aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg
     4861 taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt cttgccagcc
     4921 cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa
     4981 ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct
     5041 ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc
     5101 gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcgctgt
     5161 cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc
     5221 cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgcgg
     5281 gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc gtccaatggc
     5341 gaaataatca ggtagtcggg gccatttacg atcttgctga tgcagctgtg attgggcaaa
     5401 agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ccgccagttg
     5461 atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa aatgccaggc
     5521 cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt
     5581 gaccaggata ctcatgccgg ggcgtctccg ttatagactt cagcgaggat ctcgcgcgcg
     5641 ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatt ttgcggcgca
     5701 ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacgcagc
     5761 cagatttcac catcaataag ctcggcgtag ctaccaattg gcacctgaca tccaccttcg
     5821 agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc
     5881 gcggcaagca gctcgcgagt gcgcgtatca tcaaggcggc attcaatacc gactgcacct
     5941 tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga
     6001 cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatctagt
     6061 ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc cgggcggcgt
     6121 tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgccggtaac
     6181 gcatccagac tgtcataatt attggacaca aaggcatcgc gaggatcttc acgctcacaa
     6241 atagtgacca accccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg
     6301 gcgatatcgg cgcgattctc caggagcgcg acttccagct cttttacaaa taagcctttt
     6361 ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc
     6421 agttcaacca ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc
     6481 cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt
     6541 accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg
     6601 gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacaggc
     6661 gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag
     6721 cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt
     6781 ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg
     6841 aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta
     6901 gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc
     6961 cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt
     7021 ctacagtcta ctgccgacat tgttgcacta tcaccatccg ctaatgccgg gttaccttga
     7081 tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct
     7141 gaacgagctt gaactgtatc gtggaatgtc agtacaggac ccaccgaaag gtgagcttcc
     7201 aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga
     7261 cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttactaca
     7321 acgtaaatgt agtctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt
     7381 cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg gcgaagattg
     7441 tggctccacc cagcatatac tgctgcttga cgaattttat cgtaccgccg tgcgtctcgc
     7501 cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta
     7561 tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg
     7621 cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag
     7681 tatcgattcc ccatacaaag cggtactgaa aacactgctg ctggaagcct attcctggga
     7741 atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat
     7801 tgtatcgttt ggtctcgatc catactgcat gatgctggag cgtgttactg aatacctgac
     7861 ggcgattgaa gattttaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg
     7921 cgaaaagctc agccgtgaac gcgcctgcgt aggctggcgt cgcgcagtgt tgagccagtt
     7981 agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc gcgctaactg
     8041 gaagattgat caggtgcgtg aggcgcacaa cgagttgctc gacgcgatga tgcagagcta
     8101 ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga
     8161 tatcggcgtg ctgacgcgta agctgtatgc cgcgtttgaa gcattaccag gtaaagtgac
     8221 gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt
     8281 gccgccgggc cgtgctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga
     8341 gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaacaaac tggtggcgtg
     8401 ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag gtaacggcat
     8461 tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg
     8521 cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagatccgcc atctggcgat
     8581 tatcgttaac ctggaatatg acccgacagc ggcgttccgc aatcaggtgg tgcattttga
     8641 tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt
     8701 tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca
     8761 atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc
     8821 agatagcgtg gaagtcttct gttatagcca gcatctgcgc gggttaatcc gtactcgcgt
     8881 gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg
     8941 gcgtttcaag gcgctgcgcg tttctggtca aacctggggg ttgttcttcg aacgcctgaa
     9001 tgtatcggta caaaaactgg aaaacgccat cgagttttat ggcgctattt cgcataacaa
     9061 actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga
     9121 cggctttgct agtgaaggga tcatccagtt cttcttcgaa gaaacgcaag acgagaatgg
     9181 ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg
     9241 cagcaaagag gaactggtgc gtgacgtcag tcgcttctac tcgtcatcgc acgaccgctt
     9301 cacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt
     9361 tgatggtcgt gaacaggtga ttccgttccg cacaaaatct atcggtaaca tgccgcctgc
     9421 caatcaggat cacgatacgc cgctattaca gcaatatttt tcgtgatgaa cgtgccggaa
     9481 agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc gcttgctgtg
     9541 tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccattcat
     9601 cgccttttag gtcaaaatgg tagccgccct gtttggttgc cagccacacc tggtgcagcg
     9661 gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc agtacgccgc
     9721 cgttgatttc gcagtcgata tcgctgtcgc catcccagtc gtccaggcgt tcttcaatgg
     9781 tcagccagag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtc
     9841 tttaagtgat ggcggcagta tagcggtatg gggtcagggc ttcaaagttt gcccgtctgt
     9901 tgctgcgttc cggcatgagt catccgtcaa cggaataata atgtctctgt tgtgaagttc
     9961 acgcacattc tcttctctga cgtgtgtttt cgccgggcag ggggagggcg taatccttac
    10021 ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat cgtttttagt
    10081 tagcggaccg ttaactggaa taatttgcac gctctttgac cgtaattggc ggataaaata
    10141 agcatgctgt ttgccgctat taaatgagcg atcaatgacc aggtctggcg tatccagcgg
    10201 gattatctca agctggtctt caccaaatgt cgataatttt gttacataca accgatcttt
    10261 atattgcgca atattgccag taatcagatg gaattgttca ggttcaatat ctggtaggtc
    10321 tatccacttg ctccactgac ggtcggacgt gagctgttta cgccagcgaa cgcgactttc
    10381 ccctacttca aaagagacgt catcatctac gggtatcgct ttgccagaca attgcccaaa
    10441 gggataacga tgcataccct tattgtcgcg aaaaaccagc agtgaatgag gtatccagcg
    10501 aattatctca aatgagtctg cattcgccgt aaggtgaacg ccatttaaat aaatattctg
    10561 cccactgcgg ataagcagat cattggaata caaatttctg tttacactca taagacgtgg
    10621 attaataccc caccatcttt tttctaatac agtaacatta ctgctgctcc cctgacgtcg
    10681 accggacaaa tagagatttt tactatccac cagcgtggta gagttacctt cgatagcttt
    10741 tagtgtcgcc agatcaacac gtgacgcgcc gctattactt tcggtgcgct gtccgtcaaa
    10801 atagaggcta tatttatcga ccgcgaagcg gcccatagcc tgaaagctgg caatatcgac
    10861 agtgggtgtt cccggtggat tgctaacgat tttgccacgc cagagaataa cgcggccgtc
    10921 tgtcacccag tggtattcct cattttttat gcttatttca tcggtgggta tatagccttc
    10981 caccaactcc cgctccccgg atatatttgt cacaaggcgc aaatcggctc tgttcatctt
    11041 cggaaattgg gcaatgattt tcttatcccc ccaataacct ttaccgtaag acataacacc
    11101 gtgatcattg taatagtcga accctatggc ctgtacagca ggcgtcagaa atgtgtgtcc
    11161 caacaggatg agtgcaactg tgattatttg acccttcatt ttgtcgcctc attgcgcggg
    11221 gcaggaataa attcgccgtc atcagtaatt tttccgtcca gatatcccgt tttgtaattt
    11281 acgccatagc catatcccga atgttgttcc ttccagatta agcgaacttg tttcggatca
    11341 gcggtgcggt atcgccagag ctgcgcgcca tcaaagctgt aaatgtagcg gttatcgcgg
    11401 gcataccatc tgcgcaaaaa gggatggtca ttaagatggg cttcgcgttc atccggagaa
    11461 cggttatcca gtaagatcag cggtccgtca gattcaaaca catgtaaacc ggaatggtat
    11521 ggccaccctt cggcacgcgt aagtaaatag ccgtggcgtt tcccggcgtt gaaacgtttg
    11581 ttgataataa gattgggatc atcaagtttc acgatttcca gcttgcggtc accagactct
    11641 gtttcgatga tggtataaag cgagtcctga tactgggcga cagcatcact gatgggttga
    11701 aactgctccg gatcgagtcc gggtaattct tcctgtttac aagcaggtcc tttacgccag
    11761 aacactttct tttccagcag agaaaaatcc agacagtgct ttgttaaatc ttcatcccac
    11821 tccagatttt ccttgtcgag gactttacgc tgcagcccgt ttttatcacg ccaggtcaag
    11881 agtgagccgg gcatccagcg cacgacggaa aaggtgtccg ggtcggcatc aagctgttcg
    11941 ccgttgatca ggattttggt ccgtgtacga gccagggtat cccaggggcc aaatggcata
    12001 gccacacagg gattaaacgc ggtagaaaac tttcctcgct gatcccatga cttttgtgcc
    12061 agcacacgga aactatcggg atctgcgagg cgatgcccat tgatatacaa gaaattagcg
    12121 tcacgtaata tcagccccag taagtcagga tcccagggag gccggaattc cacctgatgc
    12181 agtgttttga tatctaagcg atttcccccg ccattgttat cggtgcgctt gccttcgaaa
    12241 tagagactgt gtttatctgc ggcaaaatca ccccacgcct tgaacgaggc gacatcgacg
    12301 ggaggtttgt ctggcgggtt ttgcacaact tcacccgcgt agaggatata gcgaccatcg
    12361 ctatgccagg agtaatgagg ataccaggcc tgtgcgcctt catagaggta gtcggttgtc
    12421 agatcagtca gaggaggtac tttatcccat ttgtctagtg ttgtcgggtc tattaataaa
    12481 ttgggtaaac ggcgcaaact tttgtaattc acgtcaggaa gagaaagatt tgtttcttta
    12541 taagggcgca ggtataccac gccttttccc tgtttctggt aaatattatg ttcaggcgga
    12601 tcaagccgac atccccatgc cgacgataaa aacaatgaac aagacaacaa acaaaccaca
    12661 ttgcgatagt gcataaagcc atcctggcgc gaggtgccga tcacgaaact accagcagaa
    12721 cataattcct cgcacgtaag cgtaactttt gcggcaaatc gtcacttttc agaatctata
    12781 aaggccatcg ctattttctg atagttgctg gggtaaactt gaggttttca ttcacgatat
    12841 aaatacgagc tactgcgcca tggtgtgact gatcacgtta aaatgtgccg gattgacgcc
    12901 gggaagggtg tcaacatgcg actctgccca ccgttaccta ctttattgtc tttcgcgtgt
    12961 tgtacttagg tcccaccacc ggtatctaac attccccaca ttattacgct ttctgccgaa
    13021 cgtgattttt atagaaatct atcaattcat tttccctgta ccataaagtt tatctttcac
    13081 atcctgccga acgcgggtct tgtacccccc gttgtgaaga tcttcatacg gagaaaaaga
    13141 atctttactc caccccagca gcactttatc gccaggcata aaatgcacat gcagaaaatc
    13201 ttctccccat tctcttttgg ggagaggtaa cgtcatattc cgtttctcca gacgcattcc
    13261 agcattatac tgtgtacgag tagtacttaa tgtccagata acctctgccg tatccccact
    13321 aacatcacca caacaggttg ttgaaccgcc accatttgca aaagcattcg cgcccgccac
    13381 gccgttaaca ctgaatccca gtatcgggcg gtcaatttca ctgtggattt ccagaatcac
    13441 tccacctctt ggtcctcccc atactgcctg tcctatattc cacaacacga ccagtaccgg
    13501 aacgacaatt aacgtccgcc atacccagcg cccccaccgg gcataaccgc gcgtaagctt
    13561 ttggtcaaat ttttccagtt gggtaaatag ccccatcaga ttttattccc tcttatcacc
    13621 aggttggcaa agttgtatgt cactggcgac taaggtcaga caacgaaatt gcatggtgcg
    13681 ccactctatt aaaagcccca tgccgacgat aagaacaatg aacaagacaa caaacaaacc
    13741 acattgcgat agtgcataaa gccatcctgg cgcgaggtgc cgatcacgaa actaccagca
    13801 aaacataaat ccccacgtgt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
    13861 ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga aagcagaaag
    13921 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattaat
    13981 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
    14041 taaaaacgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca cggtgccgga
    14101 taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
    14161 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
    14221 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
    14281 cctggctgat cggcacctgg gagtagggtt tgaccaactg ctggtggttg agccgccgta
    14341 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
    14401 gtgcggcaat ggtgcgcgct gctttgcccg ttttgtgcgt ctgaaagggc tgaccaataa
    14461 gcgcgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga
    14521 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc
    14581 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
    14641 ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt
    14701 agaaacgctt ggtcctgttc tggaaagcca cgagcgtttt ccggagcgag ccaatatcgg
    14761 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
    14821 agaaacccag gcctgcggca gcggcgcatg tgcggcggtg gcggtaggga ttcagcaagg
    14881 tttgctggcc gaagaagtac gcgtggaact ccccggcggt cgtcttgata tcgcctggaa
    14941 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
    15001 tcatctatga agcaaccagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
    15061 gcggttgtcg attatctgat taaaaatcct gagtttttta tccgtaatgc gcgcgcagta
    15121 gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga gtggcacatg
    15181 gcccgcgcac gtaatcatat tcatgttctg gaagagaaca tggcgctgtt gatggaacag
    15241 gctatcgcca acgaaggcct gttttatcgc ctactctacc tgcagcgcag tctcaccgcc
    15301 gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg
    15361 gcaggtgcga gtctgcgcct gtttccggat cgctggcgct taggtgcgcc gtcgaaccac
    15421 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tttggggcag
    15481 gaacagcact atcttgggcc gcttaacgga ccagagctgc tggtggtgct accggaagcg
    15541 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt gggtgttgtg
    15601 ctgtttacca gtcgcgatgc cagtcactat caacaagggc aaggaacgca gttacttcat
    15661 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    15721 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgccag cttagcccga
    15781 taaccctgct taactaccag cgtcagcttg aggcgatcat caattttgcc agcgaaaacg
    15841 gcctgcaaag ctggcagcaa tgtgatgtga cgatggtgcg caattttgct gtacgcagtc
    15901 gccgtaaagg gctgggagca gcaagtctgg cgttacggct ttctgcgcta cgtagctttt
    15961 ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaagga gtttcggcac
    16021 cgaaagcgcc gcgtcatctg ccgaaaaaca tcgacgtcga cgatatgaat cggctgctgg
    16081 atattgatat caacgatccc ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg
    16141 gcgcgggtct gcgtctttcc gagctggtgg ggctggatat caaacacctc gacctggagt
    16201 ctggtgaagt gtgggtaatg gggaaaggca gcaaagagcg ccgcctgccg attggtcgca
    16261 acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    16321 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    16381 ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccacat aaattacgtc
    16441 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctgc
    16501 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    16561 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    16621 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    16681 tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt atcatccggc
    16741 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagcgga
    16801 acccgagatt tatcacgacg tgacgcgttg gcgttttcgt tcgattgaac aagcgatgct
    16861 cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa tgattaactt
    16921 tgccaaatgg cgcagccgaa tcgacgtccc gcagcaaact cacgacacct taaaacagct
    16981 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg aactgtttgg
    17041 tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt
    17101 cagcgatatg tactttttgg ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca
    17161 tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcttg
    17221 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt tactgccgca
    17281 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    17341 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    17401 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacagcg cgaagcggtg
    17461 gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    17521 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    17581 gcggtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg gcaactgatg
    17641 ggcaccagcc agggcggcat gtgggtcggc accttccacg ggctggcgca ccgcctgctg
    17701 cgtgcgcacc atatggacgc caatctgccg caggatttcc agatcctcga tagcgaggac
    17761 cagctgcgcc tgcttaagcg tctgatcaaa gccatgaacc tcgacgagaa gcagtggccg
    17821 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccgcatcat
    17881 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    17941 gaagcgtgtg accgcgcggg cctggtggac ttcgccgagc tgctgctgcg cgctcacgaa
    18001 ctgtggctta acaagccgca tatcctacaa cactaccgcg aacgttttac caatatcctg
    18061 gtggacgaat tccaggatac caacaacatt cagtacgcgt ggatccgcct gctggcgggc
    18121 gacaccggca aagtgatgat cgtcggtgat gacgaccagt caatctacgg ctggcgcggg
    18181 gcgcaggtgg agaatattca gcgtttcctt aatgatttcc ccggtgccga aactattcgt
    18241 ctggagcaaa actaccgctc taccagcaat attctgagcg ccgctaacgc cctgattgaa
    18301 aacaataacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    18361 tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa
    18421 acctggcagg acaacggcgg ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc
    18481 cagtcgcgtg tactggaaga agcactgttg caggccagta tgccgtaccg tatttacggc
    18541 gggatgcgct tcttcgaacg ccaggaaatc aaagacgcgc tctcgtatct gcgtctgatt
    18601 gccaaccgta acgacgacgc ggcgtttgag cgcgtggtga atacgccaac gcggggtatt
    18661 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    18721 caggcatgtc gtgaactgtt gcaggaaaaa gccctcgccg ggcgagctgc cagcgccttg
    18781 cagcgattta tggaattaat cgatgcctta gcgcaagaaa ctgccgatat gccgctgcat
    18841 gtacagactg accgggtaat taaagactcc ggcctgcgca ccatgtacga gcaggagaag
    18901 ggcgaaaaag gccagacgcg tatcgaaaac ttagaggaac tggtgacggc aacgcgccag
    18961 ttcagctaca acgaagaaga tgaagattta atgccgctgc aggcattcct ctcccatgcg
    19021 gcgctggaag cgggcgaggg gcaggcggat acctggcagg atgcagtgca gttgatgacg
    19081 ctgcactcgg cgaaaggcct ggagttcccg caggtgttta ttgtcggtat ggaagagggt
    19141 atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg
    19201 gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc
    19261 cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttattggcga gctgccggaa
    19321 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgg
    19381 atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg cgtacgccac
    19441 gctaagtttg gtgaaggcac cattgtcaat atggaaggca gcggtgagca tagccgtttg
    19501 caggtggcat ttcagggcca gggcattaaa tggctggtgg cggcttatgc ccggctggag
    19561 tcggtgtaac gttgccggat gcggtgctgc gcaccttatt tggcctaaaa aatcattcag
    19621 attcaataaa ttgcaacgtc atgtaggccg gataaggcgt ttacgccgca tccggcatca
    19681 gcagcatatt tagtatctga cacaaaacta tcattctaac ctttccgctc gacggaaatc
    19741 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    19801 atcgtgccat caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    19861 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    19921 atcccaacac cgttacgaat tttgcgtgag gcgacgttgg gatgaatacg cggattaatt
    19981 acaccgtttt ccatcaacat tggccctgac tgcaccgcaa actgaatctc tttactggtt
    20041 ttgaaggcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    20101 ggacggataa agaaattccc ttcacctgaa gcgagattta acgccacctt ctgctgaccg
    20161 ttttcgatgt acaaaccgag cggcgcatag ctttcatcat agatgccgcc gttcatcgcc
    20221 atctgcacct gaccctgact attcatatcc gccagcagag catgtaacgt tccccacgct
    20281 tcgccattag ctttttgcca gtacattttc acccgctctg tttgaggatt aacggtatac
    20341 gcctgtacgg tcagcgtcgg atctgagagt gcgcaatcat cagcggcaac agcaaacaag
    20401 ggaagtaagg tgagggtgag aaaaatccgt ttgagattca aggtgatcat tcctttacca
    20461 atgagtagcc gatgcgccat tataggtcct ggatgttgga tttttttatc ctgttagcga
    20521 ccttgacgag taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg
    20581 ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat cactctaaga ggacattcgc
    20641 cttggacaca cccagtagat actggctcac tatcctgtca tccaggatca actcctaagg
    20701 ctatcccttt ttgctgatag ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc
    20761 tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc
    20821 cgatatttta agcattggga gtcccggtca tgctgagcgc atttcaactg gaaaataacc
    20881 gactgacccg gctggaagtc gaagagtcac aaccccttgt aaatgcagta tggattgatc
    20941 ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc tgaacttggc cagagcctgg
    21001 caacccgccc ggaactggaa gacatcgaag catcggcacg tttctttgaa gacgacgacg
    21061 gcctgcatat tcactccttc ttcttctttg aagatgcgga agatcacgcc ggtaactcca
    21121 ctgtggcatt taccatccgt gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg
    21181 cttttcgtct gtatcgtatg cgtgcccgta gccagtcgat ggtagacggt aacgcctacg
    21241 agttgctgct ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata
    21301 tctatagcga cctggagcag ttgagccggg tgattatgga agggcatcag ggcgatgagt
    21361 acgacgaggc gctctccacg ctggcggaac tggaagatat cggctggaaa gttcgcctgt
    21421 gtctgatgga tacccagcgc gcgctcaact tcctggtacg caaggcgcgt ttaccgggtg
    21481 ggcaactgga gcaggcgcgt gaaatcctgc gagatatcga atccctgctg ccgcataacg
    21541 aatccctgtt ccagaaggtg aacttcctga tgcaggcggc gatgggcttt atcaacatcg
    21601 agcagaaccg catcatcaaa atcttctcgg tggtatccgt ggtgttcctg ccaccgacgc
    21661 tggtggcttc cagctacggg atgaactttg aatttatgcc agagttgaag tggagtttcg
    21721 gctacccagg cgcgattatc tttatgatcc tcgcgggcct ggcaccgtat ctgtacttta
    21781 agcggaagaa ctggttgtaa tatgagtgcc ggataaagct atttttttat ccggcttatt
    21841 tctaaaaatt gtctttttta aagtcatgcg gattgaaaac tcgctctatt ttattttctt
    21901 gtgttaattg atactcagta aaatcgttaa taccaatatg agtgttacga taagggtatt
    21961 cattaataac cattaagtaa tttttcacga tgcaattatc attgctctta attgtcggga
    22021 aacagtagag atcttcatac ggcccttcga tatatttttt atagtcagtg aagccattac
    22081 cgatatcaat gacataccag gtatgccaac taccttctgg taggggatgt tgatttttaa
    22141 tttcattctg atgcttcaga taccaattta ttttatcgct gtcagtccat ggcagatgat
    22201 caacgaccag aaagactgaa ttgccagcat cagagtatgt atacaaaatc tgtacaggac
    22261 gcaatgtcca taaattatag atgcagcata gaagaaataa acataatata attttctgga
    22321 gtttgctttt catagataat tttcgattct ttcgatagtg ttaaaattgg tgaaaaaagg
    22381 tttgaaagca aagtctttat gccgttgtaa aaagaaccat atgcgaaaaa atctatattt
    22441 gctgtaagtc ttgtttttga tatcagttac atcaagaccg aagtggtcct gtgctacaaa
    22501 atgaattgtt gcactccatc ctatagcgta gttttgaaaa cttaaaagag atattttttg
    22561 agctgaaata tcatgaacac tgattcctaa gccattaaaa ctgtcctcga agtcattaaa
    22621 tttatttagt cttgatgaaa gtaatttggt tttaatgctt tgcaggataa gaggttggta
    22681 tcctgtcgag ggagttgaat taatgccatt ttcgatacat tctttgatta ttcgtattgg
    22741 actattataa ccatatttat ttattttttc atgaaaagat aaatttaatt gttggctatg
    22801 aaaattgctg ccatttccat atctaaaatg ttcaatcaga tcttcgataa gtgttttatt
    22861 ttgtccaaaa aaggaaaaca tttttgctag ttctttcatt tctgcaaata aaatatcagt
    22921 gcattcacgt tgtgatattt ttgttccgga acttgaggca ctaaaagcaa cattaataga
    22981 acctgggcca gggaaatggt atttaataag tctgtaagga tcaacttttg ctgagatatc
    23041 gcttaggccg agtgagagaa agtcttgttc taccatatca ccatattgca tatcatcagc
    23101 agaaaaatca tcgaataaac taatagtttc gaaaagtgta cagggaatat taattggcgg
    23161 aagtaatatg ttattcattt gcatttgcct tggttatatt taagctcaat atcaattcga
    23221 gaacaaatgc tagggtaaaa aaatatttaa aaaagaagta aacttcacat tttttcatta
    23281 catctctgaa gagaacctcg ttaatgattg gtcttgctca ttgttaataa caaaatgttg
    23341 aatttaccga ttaggaaact ttttcctacg cggattgcct ccagctatgg catgaacttt
    23401 gagtttatgc cagaactgaa gtggggcttc ggctaccctg gcgcgattat ctttatgatc
    23461 ctcgcgggcc tgacatcgta tctgtacttt aagcgtaaga actggctgta actatacgat
    23521 gtcgctgtga gtaagttagt gtgctgacag cgacatttta gccgagggaa tcgcagataa
    23581 cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg actcacatct
    23641 caccccttca attgctgcag agataataga aaaaagtgtc aagcccaatg aaatcaatac
    23701 aaagtagatt gccttacatc cagtatctct tattcttttt gctccaatat tgatatttac
    23761 ataggtatta ttaataacca gaataaaact caaagccagg ttgatatcat tcaaagttat
    23821 taatattgac catgatgtta atgtatagat tatgaactga aagcgtgata aacgctcaag
    23881 cctggatggg tagaatatat ttctgatgga tgatttgaac atttttacat tgttttatat
    23941 ataattgttt tgaatatata ttacctgaat tatgtggttt gcatatatat gcaatatagc
    24001 agtctttgca ttgataacta tacagtagta atttagagtg tgtaagtttt tattgatggg
    24061 gtatatttta attaaatata attgaaatgt atttttattc tataaacaat atcttgggtg
    24121 aatggattct atatgggaaa tggtaacttt tggcagttgt tcttgggtaa agaaccttac
    24181 agcaaaaaat ctgattttgt taaggcatgg ttattgtggt gggctattac catccttgct
    24241 ctatttattt taagcctgac tacgacatta aatttaagaa gtataggtat agggttaagt
    24301 tttgggtttt gtcaaatcct tggtagatat acgatgtctc taggatggag taagtactgg
    24361 ggagttttgg gggtatttcc tgtaacaaat attgttcttt ttcttatctt aatttttttc
    24421 ataaaaagat aaataagtca gtgggtaggc tctttaaacg gcctacccat gttaattctt
    24481 aactttttct acgctgcgta taaatcgcat ccatcacaaa aattgccagc gccacccaaa
    24541 taaaggcgaa agtcaccatc ttatcggcac ccggtttttc accataaaac gtcacagcca
    24601 gcaggaacat cagcgtcggg ccaatgtact ggaaaaagcc taacgttgag agacgcaagc
    24661 gcgtggcagc ggcggtaaaa cacaacagcg gtacggtagt gacaataccg gcggcgatca
    24721 gcagtaaatt cagcgacatc gggttttgcc ccatatggct ggttgagctg tcggcaatag
    24781 caaacaggta aattgccgcc acgggcagca gccacatggt ttcgattaac atgccggttt
    24841 gcgcttcaac ggcaatcttc ttgcgtacca gaccgtagaa ggcaaaacta aatgccagtc
    24901 ccagcgcgat aataggtagc gaaccaaaag tccacagctg gactaacacg ccacatatcg
    24961 ccagaatcac cgccagccat tgcatccggc ggaatcgctc gccgaggaaa atcatcccca
    25021 gcacaatgtt caccagcggg ttaataaagt aaccaaggct cgcttccagc atatggtgat
    25081 tgttcaccgc ccagataaac agtagccagt tgccaccaat cagcacggca gagactgcca
    25141 gcataaaaat tttctgtggc gtctgaatca gcgtttttaa ataggaccac tggcggcaaa
    25201 tgctcatcag caccaccata aagaaaaacg accagatcac gcgatgcgtc aggatttcat
    25261 cggcaggcac gtagtaaatc aacttgaagt acgctggcgc tataccccaa ataaaataag
    25321 cggcaagagc gagtaatacg ccctgccgcg tttgttttgc atccatcggg aatactcatt
    25381 tttaattggt aacagcagtt tacctgcttt tatgtcttca acctaccata taggtggcgg
    25441 tggcactggc aatataaagc tgttcttcat tgtgtaattc aacgcgggcg acggcgactt
    25501 tattgcctgc acgcaacagg ctactggtag cagtaaaacg ctcgcccctg cctgggcgca
    25561 gataatcaac gcgaagatca atggtcccca tccgcgatag ccgctggcgt agttcatctt
    25621 cactgatggt ttcgtggcgg gttaaggtac ttcccacgca caccagaccg gcggcgacat
    25681 ccagcgccga cgcaatgacc ccgccgtgca aaatgctttg cgcccagttg cccaccatca
    25741 ttggctgatt tttaaaggcc agctgtgcga actctttttc gtaacgctcc agttccatcc
    25801 ccaatgcgcg gttaaatggc atgtgataaa caaacatctc acccactaat ttcagggctt
    25861 gttcagcggt cagtacggca gacatatcat ccttacactt cattggttaa tgaaatgttg
    25921 attttatgct tctttgttgt tggtttctac tttaggaagg gataactaac ggctatggag
    25981 ttaagtatgt aaaatagccc gcagaaaaat attcacctta tcaataattc gttacggaga
    26041 acacgaccga tgcggactct gcagggctgg ttgttgccgg tgtttatgtt gcctatggca
    26101 gtatatgcac aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt
    26161 attatcgcca atatgctgca ggagcatgac aatccgttca cgctctatcc ttatgacacc
    26221 aactacctca tttacaccca aaccagcgat ctgaataaag aagcgattgc cagttacgac
    26281 tgggcggaaa atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc atttccgctg
    26341 tggcgtggga ttttaggccc gaactcggtg ttgggtgcgt cttatacgca aaaatcctgg
    26401 tggcaactgt ccaatagcga agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa
    26461 ttgttcctcg gttttgccac cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag
    26521 atggggtata accacgactc taacgggcgt tccgacccga cctcccgcag ctggaaccgc
    26581 ctttatactc gcctgatggc agaaaacggt aactggctgg tagaagtgaa gccgtggtat
    26641 gtggtgggta atactgacga taacccggat atcaccaaat atatgggtta ctaccagctt
    26701 aaaatcggct atcacctcgg tgatgcggtg ctcagtgcga aaggacagta caactggaac
    26761 accggctacg gcggcgcgga gttaggctta agttacccga tcaccaaaca tgtgcgcctt
    26821 tatactcagg tttacagcgg ctatggcgaa tcgctcatcg actataactt caaccagacc
    26881 cgtgtcggtg tgggggttat gctaaacgat ttgttttgat gaacggttga gtggttggca
    26941 aatctggaat ccagcatcca ggattaccct ctcagagact aaaagcattg cagtttctcg
    27001 cgcaggcgct gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag
    27061 gcggaagtgt tgaatctgga gtccggagct aaacaggttt tacaagaaac ctttggctac
    27121 caacagtttc gccccggcca ggaagaaatt atcgacactg tgctttccgg tcgcgattgc
    27181 ctggtcgtca tgcccaccgg tggcggaaaa tccctttgct atcaaattcc tgccttattg
    27241 ctaaacggcc ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat
    27301 caactgcaag ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac ccgcgaacag
    27361 caacttgaag tgatgacagg ctgccgcacc gggcaaattc gtctgcttta tatcgccccg
    27421 gaacgcctga tgctggataa ctttcttgag catctggcgc actggaatcc ggtgttatta
    27481 gccgttgatg aagcgcactg tatctcccaa tggggccacg atttccgccc ggaatatgcc
    27541 gcgctcggtc agttgcgcca gcggttcccg acgctgccgt ttatggcgct gaccgccaca
    27601 gccgacgaca ccacgcgcca ggatatcgtg cgcctgctgg ggctgaacga tccgctgatt
    27661 caaatcagca gttttgaccg tccgaatatt cgctacatgc tgatggagaa gttcaaaccg
    27721 ctcgatcagt tgatgcgcta cgtgcaggaa cagcgcggta agtccggcat tatctactgc
    27781 aacagccggg cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg tattagcgcg
    27841 gcggcctatc atgccgggct ggaaaataat gtccgcgccg acgtgcagga gaaattccag
    27901 cgcgatgacc tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat caataaacca
    27961 aacgttcgct tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa
    28021 accggtcgcg ccgggcgtga tggcctgccc gcggaagcga tgctgtttta cgatccggcc
    28081 gatatggcgt ggctgcgccg ttgtctggaa gagaagccgc aggggcagtt gcaggatatc
    28141 gaacgtcata aactcaatgc gatgggcgct tttgccgaag cgcaaacttg ccgtcgtctg
    28201 gtactgctga actattttgg cgaagggcgt caggagccgt gcgggaactg cgatatctgc
    28261 ctcgatccgc cgaaacagta cgacggttca accgatgctc agattgccct ttccaccatt
    28321 ggtcgtgtga atcagcgttt tggtatgggc tatgtggtgg aagtgattcg cggcgctaat
    28381 aaccagcgta tccgcgacta tggtcatgac aaactaaaag tctacggtat gggccgtgat
    28441 aaaagtcatg aacattgggt gagcgtgatc cgccagttga ttcacctcgg cctggtgacg
    28501 caaaatattg cccagcattc tgccctccaa ctgacagagg ccgcgcgtcc ggtgctgcgc
    28561 ggcgaatcct ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg
    28621 cagaaatcgt tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg caaactgcgt
    28681 aaatcgattg ccgatgaaag caatgtcccg ccgtacgtgg tgtttaacga cgcaacgttg
    28741 attgagatgg ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt
    28801 gggatgcgca agctggaacg ctttggtaaa ccgtttatgg cgcttatccg cgcgcatgtt
    28861 gacggcgacg acgaagagta gtcagcggca taaaaaagtg ccaggatgaa gactccgtaa
    28921 acgtttcccc cacgagtcaa atgtatgttg atgttatttc tcaccgtcgc catggtgcat
    28981 attgtggcgc ttatgagccc cggtcccgat ttcttttttg tctctcagac cgctgtcagt
    29041 cgttcccgta aagaagcgat gatgggcgtg ctgggcatta cctgcggtgt aatggtttgg
    29101 gctgggattg cgttgcttgg cctgcatttg attatcgaaa aaatggcctg gctgcatacg
    29161 ctgattatgg ttggcggtgg cctgtatctc tgctggatgg gttaccagat gctacgcggt
    29221 gcactgaaaa aagaggcggt ttctgcacct gcgccacagg tcgagctggc gaaaagtggg
    29281 cgcagtttcc tgaaaggttt actgaccaat ctcgctaatc cgaaagcgat tatctacttt
    29341 ggctcggtgt tctcattgtt tgtcggtgat aacgttggca ctaccgcgcg ctggggcatt
    29401 tttgcgctga tcattgtcga aacgctggcg tggtttaccg tcgttgccag cctgtttgcc
    29461 ctgccgcaaa tgcgccgtgg ttatcaacgt ctggcgaagt ggattgatgg ttttgctggg
    29521 gcgttatttg ccggatttgg cattcatttg attatttcgc ggtgatgcca gacgcgtctt
    29581 cagagtaagt cggataaggc gtttacgccg catccgacat tatttttcac gcatgcctcg
    29641 ccgatgctaa cagcgctccc accagcataa acaacgagcc gaaaatctta ttcagcgcct
    29701 tcatctgctt tggtccttta atccatagag caatccgttg agcaagggtg gcgtaaccga
    29761 tcatcacaat aatatcgacc acaatagtgg tgacgccgag cacgatatac tgcatcagtt
    29821 gcggctgttg cggcatgatg aattgcggaa atagcgccgc cagaaacaca atacttttgg
    29881 gattggtgag attcacaaaa actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg
    29941 ccagcgattt aaggtcaatt gcaccagcgg cgcgccactg ctggattccc agccaaatca
    30001 agtaagccgc gcctgcccac ttcaacactt caaacgcaat cactgagcgg gaaaatagcg
    30061 tccccaaccc cacgccaacc agcacaatat gaatcgccag tccggtctga agcccagcaa
    30121 tagacgccac cgcgccgcga taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac
    30181 cagagcctgg cgacaggctt aaaatgatcg atgtcagcag gtaggcaaac caccattcta
    30241 aggtcatgat gaactcccgg tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg
    30301 ttgtgtcagg cacgctaaaa aaaacgattt tacgtggttt aagaggcaga ttacccgatg
    30361 tttcagcagc aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga
    30421 ccgctgactg atttctggcg tcagcgtgat gaagcagagt ttactggtgt ggatgacatt
    30481 ccggtgcgct ttgtccgttt tcgcgcacag caccatgacc gggtggtagt catctgcccg
    30541 gggcgtattg agagctacgt aaaatatgcg gaactggcct acgatttgtt ccatttgggg
    30601 tttgatgtct taatcatcga ccatcgcggg cagggacgtt ccggtcgcct gttagccgat
    30661 ccgcatctcg ggcatgttaa tcgctttaat gattatgttg atgatctggc ggcattctgg
    30721 caacaggagg tccagcccgg tccgtggcgt aaacgctata tactggcaca ttcgatgggc
    30781 ggtgcgatct ccacattatt tctgcaacgc catccaggtg tatgtgacgc cattgcgcta
    30841 actgcgccaa tgtttgggat cgtgattcgt atgccgtcat ttatggcacg gcagatcctc
    30901 aactgggccg aagcgcatcc acgtttccgt gatggctatg caataggcac cgggcgctgg
    30961 cgcgcgttgc cgtttgctat caacgtactg acccacagca gacagcgata tcgacgtaac
    31021 ttacgcttct atgctgatga cccaacgatt cgcgtcggtg ggccgaccta ccattgggta
    31081 cgcgaaagta ttctggctgg cgaacaggtg ttagccggtg cgggtgatga cgccacgcca
    31141 acgcttctct tgcaggctga agaggaacgc gtggtggata accgcatgca tgaccgtttt
    31201 tgtgaactcc gcaccgccgc gggccatcct gtcgaaggag gacggccgtt ggtaattaaa
    31261 ggtgcttacc atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc
    31321 atcgttgatt ttttcaacag gcataactca cccagcggaa accgctctac agaggtttaa
    31381 atttcttatg taccaggttg ttgcgtctga tttagatggc acgttacttt ctcccgacca
    31441 tacgttatcc ccttacgcca aagaaactct gaagctgctc accgcgcgcg gcatcaactt
    31501 tgtgtttgcg accggtcgtc accacgttga tgtggggcaa attcgcgata atctggagat
    31561 taagtcttac atgattacct ccaatggtgc gcgcgttcac gatctggatg gtaatctgat
    31621 ttttgctcat aacctggatc gcgacattgc cagcgatctg tttggcgtag tcaacgacaa
    31681 tccggacatc attactaacg tttatcgcga cgacgaatgg tttatgaatc gccatcgccc
    31741 ggaagagatg cgctttttta aagaagcggt gttcaaatat gcgctgtatg agcctggatt
    31801 actggagccg gaaggcgtca gcaaagtgtt cttcacctgc gattcccatg aacaactgct
    31861 gccgctggag caggcgatta acgctcgttg gggagatcgc gtcaacgtca gtttctctac
    31921 cttaacctgt ctggaagtga tggcgggcgg cgtttcaaaa ggccatgcgc tggaagcggt
    31981 ggcgaagaaa ctgggctaca gcctgaagga ttgtattgcg tttggtgacg ggatgaacga
    32041 cgccgaaatg ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc accagcgtct
    32101 gaaagacctt catcccgagc tggaagtgat tggtactaat gccgacgacg cggtgccgca
    32161 ttatctgcgt aaactctatt tatcgtaatc gttctttatt tggtcagttg tcaacctgat
    32221 acttcgctac aatggatact cgttaatcaa agagttttcc attgtggcgc tacttatcat
    32281 caccacgatt ctgtgggcct tctcctttag cttttatggc gagtaccttg cggggcacgt
    32341 cgatagctat tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt ttctgccgtt
    32401 tctgcgtacc cgtggcaata gcctgaaaac agtcggcctg tatatgctgg tgggcgcgat
    32461 gcagcttggc gtgatgtata tgctgagttt ccgcgcttat ctctatctga cggtttccga
    32521 actgctgctg ttcaccgtac tgacgccgct ctacatcacg ctgatttatg acatcatgag
    32581 taagcgccgt ctgcgctggg gatatgcttt tagtgccttg ttggcggtga ttggtgccgg
    32641 gattattcgc tatgatcagg tcaccgacca tttctggact ggcttgttgc tggtgcaact
    32701 ctccaatatc acttttgcca tcggcatggt gggttacaaa cgcctgatgg aaactcgccc
    32761 gatgccgcag cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat
    32821 tgcatggttc ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc aatggggcat
    32881 tctggtgttt cttggcgtgg tggcttccgg gattggctac tttatgtgga actacggcgc
    32941 gacgcaggtg gacgccggaa cgctgggaat tatgaataat atgcacgttc cggcagggct
    33001 gctggtaaac ctggctatct ggcaccaaca gccgcactgg ccaacgttta ttacaggcgc
    33061 gctggtgatc ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca
    33121 aacggcagat gatcgcaggc gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc
    33181 tgacgctgct cgccatcgcg cacggcggcg tacagtcggc tccacaagcc ttcgcccagg
    33241 gttttggtca ccaccagacc ctggcgctca aaactctcta ctacccaatg cggtagcgcg
    33301 gcaataccca tccgcgcggc aaccatctga atcaacaata aggtgttatc gacgcttttc
    33361 agtgacgggc tgacgcctgc cggctgaaga aaatgccgcc agacatccag tcgactacgc
    33421 tgcaccggat aaatcaacag cgtctcgctg gcgagatctt ccggtgtaat tcgcgttttc
    33481 gccgccagtg gatggtcagg tgctaacacc agacgcactt catagtcgaa catcggcgaa
    33541 taatgcaggc cactgcgcgg caggatatcg gaggtcatca ccagatccag ctcgccctgt
    33601 tgcaacgcgg gctgcgggtc aaacgtcacg cccgatttaa aatccatctc tacctgcggc
    33661 cagttcttat ggaaattttc taacgcgggc gtcagccact gaatacagct atggcactca
    33721 atggcaatgc gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc ctggctaatt
    33781 tgcggcagca cctggtttgc cagttgcaac aggatttctc cctgcggtgt aaagcgtagc
    33841 ggctggctct tacgcacaaa tagccggaag ccaaggcgtt gttccagatc gctaaactgg
    33901 tgagacaggg cggattgcgt ctgatgcaac gtcgccgcag cggctgcgag agagccgcag
    33961 ttccgcaacg cttgtagcgt tttcaggtgt tttacttcga tcatgaaagt ccttcacttc
    34021 ggcatgaata atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca
    34081 tctggacggc taaatactaa aaattccata aggggcatat catataatga caattcttaa
    34141 tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag
    34201 ttattgggcg gggaactcca cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc
    34261 tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg gcgattttgc
    34321 ctggtacgat catgttctga ccaccagtct gctgctgggt aatgttccgc cacgtcatca
    34381 gaacaaagac ggttcggtag atatcgacac cctgttccgt attggtcgtg gacgtgcgcc
    34441 gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg tttaacacca actatcacta
    34501 catggtgccg gaattcgtta aaggccaaca gttcaaactg acctggacgc agctgctgga
    34561 ggaagtggac gaggcgctgg cgctgggcca caacgttaag cctgtgctgc tggggccggt
    34621 tacttacctg tggctgggga aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa
    34681 cgacattctg ccggtttatc agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg
    34741 ggtacagatt gatgaacccg cgctggtact ggaactacca caggcgtggc tggacgcata
    34801 caaacccgct tacgacgcgc tccagggaca ggtgaaactg ctgctgacca cctattttga
    34861 aggcgtaacg ccaaatctcg acacgattac tgcgctgcct gttcagggtc tgcatgttga
    34921 cctcgtacat ggtaaagatg acgttgctga actgcacaag cgcctgcctt ctgactggtt
    34981 gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc gccgatctta ccgagaaata
    35041 tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt
    35101 gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg
    35161 gtttgccttc gccctacaaa aatgccatga actggcactg ctgcgcgatg cgctgaacag
    35221 tggtgacacg gcagctctgg cagagtggag cgccccgatt caggcacgtc gtcactctac
    35281 ccgcgtacat aatccggcgg tagaaaagcg tctggcggcg atcaccgccc aggacagcca
    35341 gcgtgcgaat gtctatgaag tgcgtgccga agcccagcgt gcgcgtttta aactgccagc
    35401 gtggccgacc accacgattg gttctttccc gcaaaccacg gaaattcgta ccctgcgtct
    35461 ggatttcaaa aagggcaatc tcgacgccaa taactaccgc acgggcattg cggaacatat
    35521 caggcaggcc attgttgaac aggaacgttt gggactggat gtgctggtac atggcgaggc
    35581 cgagcgtaat gacatggtgg aatactttgg cgagcacctc gacgggtttg tctttacgca
    35641 aaacggttgg gtacagagct acggttcccg ctgcgtgaag ccaccgattg ttattggtga
    35701 cgttagtcgc ccggcaccga ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga
    35761 caaaccggtg aaagggatgc tgacggggcc ggtgaccata ctctgctggt cgttcccgcg
    35821 tgaagatgtc agccgtgaaa ccatcgccaa acagattgcg ctggcgctgc gtgatgaggt
    35881 ggccgatctg gaagcggcag ggattggtat catccagatt gacgaaccgg cgctgcgcga
    35941 aggtttacca ctgcgtcgta gcgactggga cgcctatctc cagtggggcg tagaggcctt
    36001 ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc cacactcaca tgtgttattg
    36061 cgagttcaac gacatcatgg attccattgc ggcgctggat gcagacgtca tcaccatcga
    36121 aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa gagtttgatt atccgaatga
    36181 aatcggtcca ggcgtctatg acattcactc gccaaacgta ccgagcgtgg aatggattga
    36241 agccctgctg aagaaagcgg caaaacgcat tccggcagag cgcctgtggg tcaacccaga
    36301 ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg gcactggcga acatggtgca
    36361 ggcggcgcag aatttgcgtc tggggtaaat ccaaaccggg tggtaatacc acccggtctt
    36421 ttctcattac agcgacttct tcccaccata ctgcttaaac cattccagca tacgctgcca
    36481 gccatctttc gcagattcgg catgatagct cgggcgataa tcagcgttga atgcatgccc
    36541 ggcatccggg tagacgataa tctctgcttt cgcattagca gcccgcagcg cctggcgcat
    36601 cgtttcaacg ctctcctgcg gaatgctgtt atcctgaccg ccatataagc cgagaaccgg
    36661 cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt gaattcagcg acttatcgcc
    36721 cgtcagtttg ccgtaccacg ccactgcggc ttttagctgt ggattatgcg cggcatacag
    36781 ccaggtgata cgtccacccc agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc
    36841 gttgcgggaa gcccaactgg cgacatgatc gagatcggcc agcacctgcg agtcaggcac
    36901 ttttgctacc agaccgctaa gcaacgtggg gatatcggca aaatcattcg gatcgccttc
    36961 gcggaagtaa agttcaggtg cgatagccag atacccctcc agcgccagac ggcgacaaat
    37021 gtcgcggata tgttcatgca cgccaaaaat ttcctgcact acaatgacca ctggcagtgg
    37081 gccatcgctt tgctttggtc tggcatggta agcaggcatg ttatcccctt gtgaagggat
    37141 agaggtgaag cccgccacaa tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg
    37201 cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc atggtattct ccgtaccctt
    37261 ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa caaatcacat tgcctgaatc
    37321 ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt tatgtgattt gaatcacttt
    37381 tggtgggtaa atttatgcaa cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt
    37441 aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa accgattcac agaggagttg
    37501 tatatgtcca agtctgatgt ttttcatctc ggcctcacta aaaacgattt acaaggggct
    37561 acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa agatcgccgc gctgatggat
    37621 aagccggtta agctggcatc tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt
    37681 aaacctgtta tcgtctgctc taccggtatc ggcggcccgt ctacctctat tgctgttgaa
    37741 gagctggcac agctgggcat tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag
    37801 ccgcatatta atgtgggtga tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg
    37861 agcctgcact tcgcaccgct ggaattcccg gctgtcgctg atttcgaatg tacgactgcg
    37921 ctggttgaag ctgcgaaatc cattggcgcg acaactcacg ttggcgtgac agcttcttct
    37981 gataccttct acccaggcca ggaacgttac gatacttact ctggtcgcgt agttcgtcac
    38041 tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa tgaactatga aatggaatct
    38101 gcaaccctgc tgaccatgtg tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt
    38161 atcgttaacc gcacccagca agagatcccg aatgctgaga cgatgaaaca aactgaaagc
    38221 caagcggtga aaatcgtggt ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc
    38281 tgaaggccga cgcgttcggc cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt
    38341 ccaactggac gtttgtacag cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag
    38401 gcgttgtgga tttctcaatc atggtttacg cagttattgc gttggtgggt gtggcaattg
    38461 gctggctgtt tgccagttat caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg
    38521 aagagatggt cgcggagtta agcgcggcaa aacaacaaat tacccaaagc gagcactggc
    38581 gtgcagagtg cgagttactc aataacgaag tgcgcagcct gcaaagtatt aacacctctc
    38641 tggaggccga tctgcgtgaa gtaaccacgc ggatggaagc cgcacagcaa catgctgacg
    38701 ataaaattcg ccagatgatt aacagcgagc agcgcctcag tgagcagttt gaaaacctcg
    38761 ccaaccgtat ttttgagcac agcaatcgcc gggttgatga gcaaaaccgt cagagtctga
    38821 acagcctgtt gtcgccgcta cgtgaacaac tggacggttt ccgccgtcag gttcaggaca
    38881 gcttcggtaa agaagcacaa gaacgccata ccctgaccca cgaaattcgc aatctccagc
    38941 aactcaacgc gcaaatggcc caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca
    39001 ataaaaccca gggcaactgg ggcgaggtag tattgacgcg ggtgctggag gcttccggtc
    39061 tgcgtgaagg gtatgaatat gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga
    39121 tgcagccgga tgtcatcgtg cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa
    39181 tgacgctggt cgcctatgaa cgctatttta acgccgaaga cgactacacc cgcgaaagcg
    39241 cgctacagga acatatcgcg tcggtgcgta accatatccg tttgctggga cgcaaagatt
    39301 atcaacagct gccggggctg cgaactctgg attacgtgct gatgtttatt cccgttgaac
    39361 ccgctttttt actggcgctt gaccgccagc cggagctgat caccgaagcg ttgaaaaaca
    39421 atatcatgct ggttagcccg actacgctgc tggtggcgtt gcgtactatt gccaacctgt
    39481 ggcgttatga gcatcaaagc cgcaacgccc agcaaatcgc cgatcgtgcc agcaagctgt
    39541 acgacaagat gcgtttgttc atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag
    39601 cgcaggataa ctaccgccag gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg
    39661 cgcaggcaga agcgtttcgc ggtttaggag tagaaattaa acgcgagatt aatccggatt
    39721 tggctgaaca ggcggtgagc caggatgaag agtatcgact tcggtcggtt ccggagcagc
    39781 cgaatgatga agcttatcaa cgcgatgatg aatataatca gcagtcgcgc tagcccatta
    39841 ggggtagtta agccgggtag aaatctaggg catcgacgcc caatctgtta cacttctgga
    39901 acaatttttt gatgagcagg cattgagatg gtggataagt cacaagaaac gacgcacttt
    39961 ggttttcaga ccgtcgcgaa ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc
    40021 gtggcatcaa aatacgatgt catgaatgat ttgatgtcat ttggtattca tcgtttgtgg
    40081 aagcgattca cgattgattg cagcggcgta cgccgtgggc agaccgtgct ggatctggct
    40141 ggtggcaccg gcgacctgac agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg
    40201 gtccttgctg atatcaatga atccatgctc aaaatgggac gcgagaagct gcgtaatatc
    40261 ggtgtgattg gcaacgttga gtatgttcag gcgaacgctg aggcgctgcc gttcccggat
    40321 aacacctttg attgcatcac catttcgttt ggtctgcgta acgtcaccga caaagataaa
    40381 gcactgcgtt caatgtatcg cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc
    40441 tcgaagccaa ttatcgagcc gctgagcaaa gcctatgatg catactcctt ccatgtgctg
    40501 ccgcgtattg gctcactggt cgcgaacgac gccgacagct accgttatct ggcagaatcc
    40561 atccgtatgc atcccgatca ggataccctg aaagccatga tgcaggatgc cggattcgaa
    40621 agtgtcgact actacaatct gacggcgggg gttgtggcgc tgcatcgtgg ttataagttc
    40681 tgacaggaga ccggaaatgc cttttaaacc tttagtgacg gcaggaattg aaagtctgct
    40741 caacaccttc ctgtatcgct cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa
    40801 agtattgcgc gtggaggtaa aaggcttttc gacgtcattg attctggtgt tcagcgaacg
    40861 ccaggttgat gtactgggcg aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc
    40921 cagtgtgttg ccgaaacttc gcgatcgcca gcagcttacc gcactgattc gcagtggtga
    40981 gctggaagtg cagggcgata ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc
    41041 agagttcgac cctgcggaac tgctggcccc ttataccggt gatatcgccg ctgaaggaat
    41101 cagcaaagcc atgcgcggag gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg
    41161 ttatgtggcg gaagccatta ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc
    41221 ctggtttgcg gaagagacgg ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct
    41281 ggaaaaactg gaggctaaat gacgccaggt gaagtacggc gcctatattt catcattcgc
    41341 acttttttaa gctacggact tgatgaactg atccccaaaa tgcgtatcac cctgccgcta
    41401 cggctatggc gatactcatt attctggatg ccaaatcggc ataaagacaa acttttaggt
    41461 gagcgactac gactggccct gcaagaactg gggccggttt ggatcaagtt cgggcaaatg
    41521 ttatcaaccc gccgcgatct ttttccaccg catattgccg atcagctggc gttattgcag
    41581 gacaaagttg ctccgtttga tggcaagctg gcgaagcagc agattgaagc tgcaatgggc
    41641 ggcttgccgg tagaagcgtg gtttgacgat tttgaaatca agccgctggc ttctgcttct
    41701 atcgcccagg ttcataccgc gcgattgaaa tcgaatggta aagaggtggt gattaaagtc
    41761 atccgcccgg atattttgcc ggttattaaa gcggatctga aacttatcta ccgtctggct
    41821 cgctgggtgc cgcgtttgct gccggatggt cgccgtctgc gcccaaccga agtggtgcgc
    41881 gagtacgaaa agacattgat tgatgaactg aatttgctgc gggaatctgc caacgccatt
    41941 cagcttcggc gcaattttga agacagcccg atgctctaca tcccggaagt ttaccctgac
    42001 tattgtagtg aagggatgat ggtgatggag cgcatttacg gcattccggt gtctgatgtt
    42061 gcggcgctgg agaaaaacgg caccaacatg aaattgctgg cggaacgcgg cgtgcaggtg
    42121 ttcttcactc aggtctttcg cgacagcttt ttccatgccg atatgcaccc tggcaacatc
    42181 ttcgtaagct atgaacaccc ggaaaacccg aaatatatcg gcattgattg cgggattgtt
    42241 ggctcgctaa acaaagaaga taaacgctat ctggcagaaa actttatcgc cttctttaat
    42301 cgcgactatc gcaaagtggc agagctacac gtcgattctg gctgggtgcc accagatacc
    42361 aacgttgaag agttcgaatt tgccattcgt acggtctgtg aacctatctt tgagaaaccg
    42421 ctggccgaaa tttcgtttgg acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc
    42481 aatatggaag tgcagccgca actggtgtta ctccagaaaa ccctgctcta cgtcgaaggg
    42541 gtaggacgcc agctttatcc gcaactcgat ttatggaaaa cggcgaagcc tttcctggag
    42601 tcgtggatta aagatcaggt cggtattcct gcgctggtga gagcatttaa agaaaaagcg
    42661 ccgttctggg tcgaaaaaat gccagaactg cctgaactgg tttacgacag tttgcgccag
    42721 ggcaagtatt tacagcacag tgttgataag attgcccgcg agcttcagtc aaatcatgta
    42781 cgtcagggac aatcgcgtta ttttctcgga attggcgcta cgttagtatt aagtggcaca
    42841 ttcttgttgg tcagccgacc tgagtggggg ctgatgcccg gctggttaat ggcaggtggt
    42901 ctgatcgcct ggtttgtcgg ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg
    42961 gccgtgtaac gtataatgcg gctttgttta atcatcatct accacagagg aacatgtatg
    43021 ggtggtatca gtatttggca gttattgatt attgccgtca tcgttgtact gctttttggc
    43081 accaaaaagc tcggctccat cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa
    43141 gcaatgagcg atgatgaacc aaagcaggat aaaaccagtc aggatgctga ttttactgcg
    43201 aaaactatcg ccgataagca ggcggatacg aatcaggaac aggctaaaac agaagacgcg
    43261 aagcgccacg ataaagagca ggtgtaatcc gtgtttgata tcggttttag cgaactgcta
    43321 ttggtgttca tcatcggcct cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa
    43381 acggtagcgg gctggattcg cgcgttgcgt tcactggcga caacggtgca gaacgaactg
    43441 acccaggagt taaaactcca ggagtttcag gacagtctga aaaaggttga aaaggcgagc
    43501 ctcactaacc tgacgcccga actgaaagcg tcgatggatg aactacgcca ggccgcggag
    43561 tcgatgaagc gttcctacgt tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc
    43621 atccataacc cggtggtgaa agataatgaa gctgcgcatg agggcgtaac gcctgccgct
    43681 gcacaaacgc aggccagttc gccggaacag aagccagaaa ccacgccaga gccggtggta
    43741 aaacctgctg cggacgctga accgaaaacc gctgcacctt ccccttcgtc gagtgataaa
    43801 ccgtaaacat gtctgtagaa gatactcaac cgcttatcac gcatctaatt gagctgcgta
    43861 agcgtctgct gaactgcatt atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg
    43921 ccaatgacat ctatcacctg gtatccgcgc cattgatcaa gcagttgccg caaggttcaa
    43981 cgatgatcgc caccgacgtg gcctcgccgt tctttacgcc gatcaagctg acctttatgg
    44041 tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt gtgggcattt atcgccccag
    44101 cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct ggtttccagc tctctgctgt
    44161 tttatatcgg catggcgttc gcctactttg tggtctttcc gctggcattt ggcttccttg
    44221 ccaataccgc gccggaaggg gtacaggtat ccaccgacat cgccagctat ttaagcttcg
    44281 ttatggcgtt gtttatggcg tttggtgtct cctttgaagt gcctgtggca attgtgctgc
    44341 tgtgctggat ggggattacc tcgccagaag acttacgcaa aaaacgcccg tatgtgctgg
    44401 ttggtgcatt cgttgtcggg atgctgctga caccgccgga tgtcttctcg caaacgctgt
    44461 tggcgatccc gatgtactgt ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg
    44521 gtaaagggcg aaatcgggaa gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag
    44581 aataaattca accgcccgtc agggcggttg tcatatggag tacaggatgt ttgatatcgg
    44641 cgttaatttg accagttcgc aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt
    44701 tgacgcggga gttaatgggc tactcatcac cggcactaac ctgcgtgaaa gccagcaggc
    44761 gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg gcgggcgtac atcctcacga
    44821 cagcagccag tggcaagctg cgactgaaga agcgattatt gagctggccg cgcagccaga
    44881 agtggtggcg attggtgaat gtggtctcga ctttaaccgc aacttttcga cgccggaaga
    44941 gcaggaacgc gcttttgttg cccagctacg cattgccgca gatttaaaca tgccggtatt
    45001 tatgcactgt cgcgatgccc acgagcggtt tatgacattg ctggagccgt ggctggataa
    45061 actgcctggt gcggttcttc attgctttac cggcacacgc gaagagatgc aggcgtgcgt
    45121 ggcgcgtgga atttatatcg gcattaccgg ttgggtttgc gatgaacgac gcgggctgga
    45181 gctgcgggaa ttgttgccgt tgattccggc ggagaaattg ctgatcgaaa ctgatgcgcc
    45241 gtatctgctc ccgcgcgatc tcacgccaaa gccatcatcc cggcgcaacg agccagccca
    45301 tctgccccat attttgcaac gtattgcgca ctggcgtgga gaagatgccg catggctggc
    45361 tgccaccacg gatgccaatg tcaaaacact gtttgggatt gcgttttaga gtttgcggaa
    45421 ctcggtgttc ttcacactgt gcttaatctc tttattaata agattaagca atagcatgga
    45481 gcgagcctca ccatcgggtt cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt
    45541 aataatcacc ttatcacccg gataaggggt tgccggatcg acaatgtctt tcggtttata
    45601 taccgatagc tgatgaatca ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac
    45661 gaagtggctg acaccgcggg tcgcgttgat agtcgtggta tgaatcactt ctggatcaaa
    45721 ttcgacaaac aggtagttgg gaaacaatgg ctcactgact gcagtacgtt ttccacgcac
    45781 gattttttcc agggtgatca tcggtgccag gcaattcaca gcctgtcttt cgaggtgttc
    45841 ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa taccaggatt gcataatgac
    45901 tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt tacgctaagt taattatatt
    45961 ccccggtttg cgttataccg tcagagttca cgctaattta acaaatttac agcatcgcaa
    46021 agatgaacgc cgtataatgg gcgtagatta agaggctaca atggacgcca tgaaatataa
    46081 cgatttacgc gacttcctga cactgcttga acagcagggt gagctaaaac gtatcacgct
    46141 cccggtggat ccgcatctgg aaatcactga aattgctgac cgcactttgc gtgccggtgg
    46201 gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg ccggtgctgt gcaacctgtt
    46261 cggtacgcca aagcgcgtgg caatgggcat ggggcaggaa gatgtttcgg cgctacgtga
    46321 agttggtaaa ttattggcgt ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct
    46381 gtttgataaa ctgccgcagt ttaagcaagt attgaacatg ccgacaaagc ggctgcgtgg
    46441 tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc gatctcaatc gcattcccat
    46501 tatgacctgc tggccggaag atgccgcgcc gctgattacc tgggggctga cagtgacgcg
    46561 cggcccgcat aaagagcggc agaatctggg catttatcgc cagcagctga ttggtaaaaa
    46621 caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg
    46681 tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg ctgggtgccg atcccgccac
    46741 gattctcggt gcagtcaccc ccgttccgga tacactttca gagtatgcgt ttgccggatt
    46801 gctgcgcggt accaagaccg aagtggtgaa gtgtatctcc aatgaccttg aagtgcccgc
    46861 cagtgcggag attgtgctgg aagggtatat cgaacaaggt gaaacggcgc cggaagggcc
    46921 gtatggcgac cacaccggtt actataacga agtcgatagt ttccctgtct ttactgtgac
    46981 gcatattacc cagcgtgaag atgcgattta ccattccacc tataccgggc gtccgcctga
    47041 tgaacctgcg gtactgggcg tggcgttgaa cgaagtgttt gtgccgattc tgcaaaaaca
    47101 gttcccggaa attgtcgatt tttacctgcc accggaaggc tgttcttatc gtctggcggt
    47161 agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc
    47221 gttcttacgc cagtttatgt acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc
    47281 acgcgactgg aacgatgtga tttgggcgat taccacccgt atggacccgg cgcgggatac
    47341 tgttctggta gaaaatacgc ctattgatta tctggatttt gcctcgcctg tctccgggct
    47401 gggttcaaaa atggggctgg atgccacgaa taaatggccg ggggaaaccc agcgtgaatg
    47461 gggacgtccc atcaaaaaag atccagatgt tgtcgcgcat attgacgcca tctgggatga
    47521 actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc
    47581 gatccgacag agaaagcgca tgacaacctt aagctgtaaa gtgacctcgg tagaagctat
    47641 cacggatacc gtatatcgtg tccgcatcgt gccagatgcg gccttttctt ttcgcgctgg
    47701 tcagtatttg atggtagtga tggatgagcg cgacaaacgt ccgttctcaa tggcttcgac
    47761 gccggatgaa aaagggttta tcgagctgca tattggcgct tctgaaatca acctttacgc
    47821 gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc gtggtcgata ttccccacgg
    47881 agaagcgtgg ctgcgcgatg atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg
    47941 gttctcttat gcccgctcga ttttgctgac agcgttggcg cgtaacccaa accgtgatat
    48001 caccatttac tggggcgggc gtgaagagca gcatctgtat gatctctgcg agcttgaggc
    48061 gctttcgttg aagcatcctg gtctgcaagt ggtgccggtg gttgaacaac cggaagcggg
    48121 ctggcgtggg cgtactggca ccgtgttaac ggcggtattg caggatcacg gtacgctggc
    48181 agagcatgat atctatattg ccggacgttt tgagatggcg aaaattgccc gcgatctgtt
    48241 ttgcagtgag cgtaatgcgc gggaagatcg cctgtttggc gatgcgtttg catttatctg
    48301 agatataaaa aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttattcagt
    48361 ggcatttaga tctatgtcgc atccggcatt cggcacacga tgcctgatgc gacgctggcg
    48421 cgtcttatca ggcctacatt ggtgccggat cggtagaccg gataaggcgt tcacgccgca
    48481 tccggcaagt ggttaaaccc gctcaaacac cgtcgcaata ccctgaccca gaccgataca
    48541 catcgtcgcc agaccaaact ggacgtcttt gcgctccatc agatttagca gcgtggtgct
    48601 gatacgcgca ccggaacagc ccagcggatg acccagcgcg atcgcgccac cgttgaggtt
    48661 gatcttctcg tcaatctgct ccattagccc cagatcttta atacatggca ggatctgcgc
    48721 ggcaaaggct tcattcattt caaacacgcc gatatcactg acagaaagcc ccgctttttt
    48781 cagcgccagc ttcgaggccg gaaccgggcc gtaacccata atcgatgggt cacaaccaac
    48841 gaccgccatc gaacgcacgc gggcgcgcgg cttaagacct aattcatggg cgcggctttc
    48901 actcatcacc agcatggcag ctgcgccatc ggaaagcgca gaagatgtac ccgccgttac
    48961 cgtaccgctt actggatcaa acgccggacg cagcgtggcg agggcttcca cggtggtttc
    49021 cgggcgaatc acttcgtcgt aattaaactg cttcaggacg ccgtcggcat cgtgaccacc
    49081 ggtcgggata atttcatttt taaatgcacc cgactgcgtg gcggcccagg cgcgggcgtg
    49141 tgaccgcgcg gcaaaggcat cctgcatttc acggctgata ccgtgcatac gcgccagcat
    49201 ttctgccgtt aagcccatca tgcccgccgc tttggcgaca ttgcggctca ggccggggtg
    49261 aaaatcgacg ccgtggctca ttggcacatg gcccatatgc tccacgccgc cgacaagaca
    49321 tgcctgcgca tcgccagtca tgatcattcg tgctgcgtca tgcaatgcct gcatggatga
    49381 accacacaag cgattgacgg tcaccgccgg gacagagtgt ggtacttctg ccagcagcgc
    49441 cgcgttacgg gcgatattaa aaccctgctc cagcgtctgc tgcacacaac cccagtaaat
    49501 atcgtcgagg gccgccgctt ccagcgccgg gttacgcgcc agcaggctac gcattaaatg
    49561 agcggagaga tcttctgcac gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg
    49621 ggtgcgaatt gcatcgacaa tgacaacctg ttccattgtg actccttaag ccgttttcag
    49681 gtcgccaacc ggacgggctg gctcaaccgg aggatagtac ggttcgttat gacgcgcttt
    49741 attacgcaga ccttccggca cttcatacag cgggccgagg tgctgatatt gctgtgccat
    49801 atcgaggtat tttgcgctac cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg
    49861 agggaagccc aggccgtaga ccagcgccat atccgcttcc gccggagtgg cgataatgcc
    49921 ttcctccaga cagcgcacca cttcgttgac catcgggatc atcatgcggg cgataatctc
    49981 ttcttcgctg aaatcgcgct tcggctggct cacttctgcc agcaggtctt caacggcggc
    50041 gtcttcttct ttcttcggct tacctttgct gtcttcttta taacgccaga aaccgaggcc
    50101 gttcttctga ccaaagcggt tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt
    50161 ctgcatccgc tgcgggaagc ctgctgccat gacagcctga gcgtgatgcg cggtatcaat
    50221 gcccacgaca tccagcagat atgccggacc cataggccag ccaaactgtt tttccatcac
    50281 tttgtcgatc ttgcggaaat ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata
    50341 cgggaacagc acgcggttaa caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt
    50401 gcccatcttg ctcgcccagg cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc
    50461 gcgaataatt tctaccaacg gcatccggtg taccgggtta aaaaagtgca ttccgcagaa
    50521 gttttccggg cgttccagcg cgttggccag ttcgctgata ggaatggttg aagtgttaga
    50581 cgccagcacg gtatccgggc gtactttttg ttcggtttct gccagtacgg cttttttcac
    50641 tttcgggttt tcgacaaccg cttctaccac aacatccacg cggtcaaacc cggcgtagtc
    50701 gagcgttggg tggattgtgg agatcacgcc agccagtttc agaccatcga tcttgccgcg
    50761 ctcaagctgc ttgttcagca gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc
    50821 gttgatatct ttcatgacaa ccggcacgcc tttccacgca gactggtaag cgatgccgcc
    50881 gcccataatg cctgcaccca gcaccgcggc ctgtttcggg gtttcaacgt ctttggtgag
    50941 tttcttcgct ttgcctttta catattgatc gttaaggaaa atgccgacca gtgcgcgggc
    51001 ttcgttggta tgcgccagcg ggacaaaact tttgttttcc aggtttaagg cttcttcacg
    51061 accaaaacgg gccgcagctt caatggtttt tactgcggtg atgggggccg gataatgttt
    51121 ccccgctgtt tgtgcgacca tccctttagc gatggtgaag ctcatggtgg cttcaatctt
    51181 gctcagtttt agtggttcca gcttcggctg acgttttgct ttccagtcga ggtcgccgtt
    51241 aatggcctgg cgtaaaaccg cctttgcgcc ttcaaccagt ttttctgctt tgactacgcc
    51301 atccaccaga ccgattttca gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat
    51361 ttccagcgca ctgtcagcgc ccagcatacg tggcatacgt acagaaccgc caaagcccgg
    51421 catgatgccc agtttggttt ccggcagacc gatgcgcaga tccggcgtcg ccagacgata
    51481 atcggtcgcc agcacgcatt cgcagccacc gcccagcgca tagccgttgg cggcagcaat
    51541 ggtcggcacc ggcagatctt ccaggcgatt aaacacgcta ttggcaaagt gcagccactg
    51601 acttaactgt tcttcaggaa cgaggaacag ggacaaaaat tcggtgatat cagcaccgac
    51661 gataaaggct gctttgttcg aacgcagcag cagccctttt agatctgatt gctgttccag
    51721 cacgccgatg gcctcgccga ggctggcgac ggtcgcagtg tcgagtttat taactgaacc
    51781 tggggcatca aataccagtt cggcaatgcc atcttccagc cagtcaaggt acagggtgtc
    51841 gcctttgtaa agcatgtcag tctcctgaat ccgcaaggtg atctggtcgt accagatgag
    51901 tcgaagtgtg tattttgtgt taaaaatatg caaataaaag attaaagaaa tgccgatctg
    51961 atcacgctcg gcagaaatca cgctctggat gaacgatgtg ctaagatgcg gagacttaag
    52021 gtcaaaaaaa cagaagggta aaaaatggaa tcactggcct cactctataa aaatcatata
    52081 gctaccttac aggaacggac tcgcgatgcg ctggcgcgct tcaagctgga tgcgttactt
    52141 attcactccg gcgaactgtt caacgttttt ctcgacgatc atccctatcc gtttaaagtg
    52201 aacccgcaat tcaaagcgtg ggtgccggta actcaggtgc caaactgctg gctgctggtg
    52261 gatggcgtga acaagccgaa actgtggttc tatctgccgg ttgattactg gcacaacgtc
    52321 gaaccgctgc cgacctcttt ctggactgaa gacgtggaag tgatcgcgct gccgaaagcc
    52381 gatggcattg gtagcctgct gcctgctgcg cgcgggaata tcggttatat cggtccggtg
    52441 ccggaacgtg cgctgcaact gggtattgag gccagcaata tcaacccgaa aggggtgatc
    52501 gactacctgc attactaccg ctccttcaaa accgagtacg aactggcctg tatgcgtgaa
    52561 gcgcagaaaa tggcggtcaa cggtcatcgc gcggcagaag aagcgttccg ttctggcatg
    52621 agcgagttcg atatcaacat tgcctatctg accgcgaccg gtcatcgtga taccgacgta
    52681 ccttacagca acattgtggc gctcaacgaa cacgctgcgg tgttgcatta caccaaactg
    52741 gaccaccagg caccggaaga gatgcgcagc ttcctgctgg atgccggggc cgaatataac
    52801 ggctatgcgg ctgacctgac ccgtacctgg tcggcaaaaa gtgacaacga ctacgcacag
    52861 ctggtgaaag acgtaaatga tgaacaactg gcgctgatcg ctaccatgaa agcaggcgtc
    52921 agctatgtgg attaccacat ccagttccat cagcgcatcg ccaaactgct gcgtaaacat
    52981 caaatcatca ccgatatgag tgaagaagcg atggtcgaaa acgatctcac cggaccgttt
    53041 atgccgcacg gtattggcca tccgctgggc ctgcaggtgc atgacgtagc cggttttatg
    53101 caggatgata gcggtacgca cctcgcggca ccggcaaaat atccgtacct gcgctgcacc
    53161 cgtattctcc agccgggcat ggtgttaacc atcgaaccgg gtatctactt cattgaatcg
    53221 ctgctggcac cgtggcgtga agggcagttc agcaagcact tcaactggca gaaaattgaa
    53281 gcactgaaac cgttcggcgg cattcgtatc gaagacaacg tggtgatcca cgaaaacaac
    53341 gtggaaaaca tgacccggga tctgaaactg gcgtgatgga aagctggtta attcctgcgg
    53401 caccggtcac ggtcgttgaa gagatcaaaa aaagccgctt cataacgatg ttggcgcata
    53461 ccgatggcgt tgaggcggcg aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg
    53521 cccgccacca ttgcgtggcg tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt
    53581 tctctgacga cggggagccg gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg
    53641 gcagcggcgt cggggaaatt accgctgtgg tagtgcgcta ctacggcggc atattgcttg
    53701 gcaccggtgg gttagtgaaa gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa
    53761 cgacccaacg caagacgcca ttaaccgaat atactttgca atgtgaatat agtcagttaa
    53821 ccggcattga agcgttgctg gggcagtgtg acggcaaaat tatcaacagt gattatcagg
    53881 cattcgttct gctgcgggtg gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc
    53941 tggtggattt tagccgtggt tcattgcaat tgttagcgat tgaagaataa tccccacttc
    54001 gttttgcaga actaaggaag cggcagagat gcattttcgc gccattaccc gaatcgttgg
    54061 actactggtc atcttatttt cagggaccat gattatccct gggctggtag cactcatcta
    54121 ccgggatgga gcgggccgcg cttttaccca gacctttttt gtcgccctcg ccattggctc
    54181 tatgctgtgg tggccgaacc gcaaagagaa gggcgaactg aaatcccgtg aggggtttct
    54241 gatagtggtg ctgttttgga ccgtgctggg tagcgtcggt gcgctccctt ttatcttctc
    54301 ggaaagcccg aacctcacga ttaccgatgc gttttttgaa tctttctctg gcctgaccac
    54361 caccggggcc actacgctgg tggggctgga ttcgctccct catgccatcc tcttttatcg
    54421 ccagatgctg caatggtttg gcgggatggg gatcatcgtg ttagcggttg cgatactgcc
    54481 tatcctcggc gtgggtggga tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga
    54541 taacaaaatg cgcccgcgaa ttgcggaaac ggcgaaaacc ctgtggttga tttatgtctt
    54601 gctgaccgtc gcctgtgcgc tggcgttgtg gtttgccgga atggatgcct ttgatgccat
    54661 cggccatagc tttgcgacta tcgctattgg cggcttctcg acacatgatg ccagtatcgg
    54721 ttatttcgac agcccgacta ttaacactat cattgctatc ttcctgctga tctccggctg
    54781 taactacggt ctgcactttt cactgttaag tgggcgtagt ctgaaggttt attggcgcga
    54841 tccggaattt cgcatgttta tcggcgtaca gtttacgctg gtggttattt gtacactcgt
    54901 actgtggttt cataatgtct acagttcggc gctgatgaca attaaccagg cgtttttcca
    54961 ggtggtatcg atggcgacaa ccgccgggtt tacgactgac agcattgccc gctggccgct
    55021 ctttttgccg gtactgcttt tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg
    55081 cggtggcctg aaagtgatcc gcatcctgct gctgtttaag caggggaacc gtgagctgaa
    55141 acgactggtg catccgaacg ccgtctatag cattaagctg gggaatcgcg cactgccgga
    55201 acgtatcctc gaagccgttt ggggattttt ctccgcctat gcattggtgt ttattgtcag
    55261 tatgctggcg attatcgcca cgggcgtgga tgacttttct gcctttgcct cagttgttgc
    55321 gacattgaat aacctggggc cagggcttgg cgtggttgct gataacttta ccagtatgaa
    55381 cccggtggct aaatggatcc tgattgccaa catgctgttt ggtcgtctcg aggtctttac
    55441 attgctggtg ctctttaccc cgactttctg gcgtgaatga tggagtaata cgtgaaaaca
    55501 ttaattcttt tctcaacaag ggacggacaa acgcgcgaga ttgcctccta cctggcttcg
    55561 gaactgaaag aactggggat ccagacggat gtcgccaatg tgcaccgcat tgaagaacca
    55621 caatgggaaa attatgaccg tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac
    55681 tctgcgttcc aggaatttgt caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc
    55741 ttttattccg taaacctggt ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc
    55801 tacgcgcgga agtttctgat gaactcgcaa tggcgtcccg atcactgcgc ggtcattgcc
    55861 ggggcgctgc gttacccacg ttatcgctgg tacgatcgtt ttatgatcaa gctaattatg
    55921 aagatgtcag gcggtgaaac ggatacgcgc aaagaagttg tctataccga ttgggagcag
    55981 gtggcgaatt tcgcccgaga aatcgcccat ttaaccgaca aaccgacgct gaaataagca
    56041 taaagaataa aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa
    56101 taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgcc gccgggtcag
    56161 cggggttctc ctgagaatct caacagagaa aagtaaaaaa tgcttgactc tgtagcggga
    56221 aagtgtatta tgcacacccc gcgccgctga gaaaaagcga agcggcactg ctctttaaca
    56281 atttatcaga caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacgaa
    56341 aaatgaatac caagtctcaa gagtgaacac gtaattcatt acgaagttta attctttgag
    56401 catcaaactt ttaaattgaa gagtttgatc atggctcaga ttgaacgctg gcggcaggcc
    56461 taacacatgc aagtcgaacg gtaacaggaa gaagcttgct tctttgctga cgagtggcgg
    56521 acgggtgagt aatgtctggg aaactgcctg atggaggggg ataactactg gaaacggtag
    56581 ctaataccgc ataacgtcgc aagaccaaag agggggacct tcgggcctct tgccatcgga
    56641 tgtgcccaga tgggattagc ttgttggtgg ggtaacggct cacc
//
LOCUS       DAHBHC010000023        55902 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHBHC010000023 DAHBHC010000000
VERSION     DAHBHC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.023"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..362
                     /locus_tag="M2A35_001795"
     CDS             <1..362
                     /locus_tag="M2A35_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCB7198107.1"
                     /translation="RFRTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLA
                     AMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAH
                     RENPAGVIASLAQILRF"
     gene            377..1234
                     /gene="menB"
                     /locus_tag="M2A35_001796"
     CDS             377..1234
                     /gene="menB"
                     /locus_tag="M2A35_001796"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCB7198108.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1234..2196
                     /gene="menC"
                     /locus_tag="M2A35_001797"
     CDS             1234..2196
                     /gene="menC"
                     /locus_tag="M2A35_001797"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCB7198109.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2193..3548
                     /gene="menE"
                     /locus_tag="M2A35_001798"
     CDS             2193..3548
                     /gene="menE"
                     /locus_tag="M2A35_001798"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCB7198110.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3658..3924
                     /gene="pmrD"
                     /locus_tag="M2A35_001799"
     CDS             3658..3924
                     /gene="pmrD"
                     /locus_tag="M2A35_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCB7198111.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(3918..4304)
                     /gene="arnF"
                     /locus_tag="M2A35_001800"
     CDS             complement(3918..4304)
                     /gene="arnF"
                     /locus_tag="M2A35_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCB7198112.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(4304..4639)
                     /gene="arnE"
                     /locus_tag="M2A35_001801"
     CDS             complement(4304..4639)
                     /gene="arnE"
                     /locus_tag="M2A35_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCB7198113.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4636..6288)
                     /gene="arnT"
                     /locus_tag="M2A35_001802"
     CDS             complement(4636..6288)
                     /gene="arnT"
                     /locus_tag="M2A35_001802"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCB7198114.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6288..7178)
                     /locus_tag="M2A35_001803"
     CDS             complement(6288..7178)
                     /locus_tag="M2A35_001803"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCB7198115.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7175..9157)
                     /gene="arnA"
                     /locus_tag="M2A35_001804"
     CDS             complement(7175..9157)
                     /gene="arnA"
                     /locus_tag="M2A35_001804"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCB7198116.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9157..10125)
                     /gene="arnC"
                     /locus_tag="M2A35_001805"
     CDS             complement(9157..10125)
                     /gene="arnC"
                     /locus_tag="M2A35_001805"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCB7198117.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10129..11268)
                     /gene="arnB"
                     /locus_tag="M2A35_001806"
     CDS             complement(10129..11268)
                     /gene="arnB"
                     /locus_tag="M2A35_001806"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCB7198118.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11576..12178
                     /locus_tag="M2A35_001807"
     CDS             11576..12178
                     /locus_tag="M2A35_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCB7198119.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12217..12642)
                     /gene="nudI"
                     /locus_tag="M2A35_001808"
     CDS             complement(12217..12642)
                     /gene="nudI"
                     /locus_tag="M2A35_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCB7198120.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVAARKTLRLKGLL"
     gene            12921..13463
                     /locus_tag="M2A35_001809"
     CDS             12921..13463
                     /locus_tag="M2A35_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198121.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     GGNRDNAVADGPYVGVNASF"
     gene            13563..14765
                     /locus_tag="M2A35_001810"
     CDS             13563..14765
                     /locus_tag="M2A35_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCB7198122.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            14820..15290
                     /locus_tag="M2A35_001811"
     CDS             14820..15290
                     /locus_tag="M2A35_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCB7198123.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(15331..16233)
                     /locus_tag="M2A35_001812"
     CDS             complement(15331..16233)
                     /locus_tag="M2A35_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCB7198124.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(16427..17617)
                     /gene="glpC"
                     /locus_tag="M2A35_001813"
     CDS             complement(16427..17617)
                     /gene="glpC"
                     /locus_tag="M2A35_001813"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCB7198125.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQMLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(17614..18873)
                     /gene="glpB"
                     /locus_tag="M2A35_001814"
     CDS             complement(17614..18873)
                     /gene="glpB"
                     /locus_tag="M2A35_001814"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCB7198126.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(18863..20491)
                     /gene="glpA"
                     /locus_tag="M2A35_001815"
     CDS             complement(18863..20491)
                     /gene="glpA"
                     /locus_tag="M2A35_001815"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCB7198127.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVAAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            20764..22122
                     /gene="glpT"
                     /locus_tag="M2A35_001816"
     CDS             20764..22122
                     /gene="glpT"
                     /locus_tag="M2A35_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCB7198128.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            22127..23203
                     /gene="glpQ"
                     /locus_tag="M2A35_001817"
     CDS             22127..23203
                     /gene="glpQ"
                     /locus_tag="M2A35_001817"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCB7198129.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(23245..23484)
                     /locus_tag="M2A35_001818"
                     /pseudo
     CDS             complement(23245..23484)
                     /locus_tag="M2A35_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            23666..24316
                     /gene="inaA"
                     /locus_tag="M2A35_001819"
     CDS             23666..24316
                     /gene="inaA"
                     /locus_tag="M2A35_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCB7198130.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(24370..24624)
                     /gene="yfaE"
                     /locus_tag="M2A35_001820"
     CDS             complement(24370..24624)
                     /gene="yfaE"
                     /locus_tag="M2A35_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCB7198131.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(24624..25754)
                     /gene="nrdB"
                     /locus_tag="M2A35_001821"
     CDS             complement(24624..25754)
                     /gene="nrdB"
                     /locus_tag="M2A35_001821"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCB7198132.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(25901..28186)
                     /gene="nrdA"
                     /locus_tag="M2A35_001822"
     CDS             complement(25901..28186)
                     /gene="nrdA"
                     /locus_tag="M2A35_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCB7198133.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            28882..32616
                     /gene="yfaL"
                     /locus_tag="M2A35_001823"
     CDS             28882..32616
                     /gene="yfaL"
                     /locus_tag="M2A35_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCB7198134.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQGSVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRCSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(32744..33466)
                     /locus_tag="M2A35_001824"
     CDS             complement(32744..33466)
                     /locus_tag="M2A35_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCB7198135.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            33613..36240
                     /gene="gyrA"
                     /locus_tag="M2A35_001825"
     CDS             33613..36240
                     /gene="gyrA"
                     /locus_tag="M2A35_001825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCB7198136.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            36389..38077
                     /locus_tag="M2A35_001826"
     CDS             36389..38077
                     /locus_tag="M2A35_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCB7198137.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            38074..38697
                     /locus_tag="M2A35_001827"
     CDS             38074..38697
                     /locus_tag="M2A35_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCB7198138.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            38721..43235
                     /locus_tag="M2A35_001828"
     CDS             38721..43235
                     /locus_tag="M2A35_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCB7198139.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            43236..44885
                     /locus_tag="M2A35_001829"
     CDS             43236..44885
                     /locus_tag="M2A35_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCB7198140.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            44890..45666
                     /locus_tag="M2A35_001830"
     CDS             44890..45666
                     /locus_tag="M2A35_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCB7198141.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            45941..48790
                     /gene="rcsC"
                     /locus_tag="M2A35_001831"
     CDS             45941..48790
                     /gene="rcsC"
                     /locus_tag="M2A35_001831"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCB7198142.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEVKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(48990..49640)
                     /gene="rcsB"
                     /locus_tag="M2A35_001832"
     CDS             complement(48990..49640)
                     /gene="rcsB"
                     /locus_tag="M2A35_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCB7198143.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLSKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49657..52329)
                     /gene="rcsD"
                     /locus_tag="M2A35_001833"
     CDS             complement(49657..52329)
                     /gene="rcsD"
                     /locus_tag="M2A35_001833"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCB7198144.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            53067..54170
                     /gene="ompC"
                     /locus_tag="M2A35_001834"
     CDS             53067..54170
                     /gene="ompC"
                     /locus_tag="M2A35_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCB7198145.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            54282..55337
                     /gene="apbE"
                     /locus_tag="M2A35_001835"
     CDS             54282..55337
                     /gene="apbE"
                     /locus_tag="M2A35_001835"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCB7198146.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSTLMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            55411..>55902
                     /locus_tag="M2A35_001836"
     CDS             55411..>55902
                     /locus_tag="M2A35_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7198147.1"
                     /translation="MKKATCLTDDQCWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVVMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSS"
BASE COUNT        13571 a        13787 c        15005 g        13539 t
ORIGIN      
        1 agcgttttcg cacagaaccg ttaacggcgg tatttgccga ctggtatcaa cagcctgttt
       61 ttgcctcgct caatgacgat caacgccggg agctggtggc gctgcgcagc aacaataatg
      121 gcgcaaccct tgccgccatg ctggaggcga cttctctcgc cgtccagcct gatttacgtg
      181 ctaaccttag cgcccgcaca tttgcgtttt attatttatg tggtgaacgt gacagcaaat
      241 tccgcgccct ggcggcggaa ctggctgccg actgccatgt cattcctcgc gccggacata
      301 acgcgcatcg ggaaaatccc gctggcgtaa tcgcaagtct ggcgcagatc ttgcgtttct
      361 gactaaagga cacaatatga tttatcctga tgaagcaatg ctttacgcac cggttgaatg
      421 gcacgactgc tccgaaggtt tcgaggacat tcgttatgaa aaatccaccg acggtatcgc
      481 aaaaatcacc attaatcgtc cgcaggtgcg caatgccttc cgtcctctga cggtaaaaga
      541 gatgatccag gcactggcag atgcgcgtta tgacgacaat atcggcgtga tcattctgac
      601 tggcgcaggc gataaagcgt tctgctccgg tggtgaccag aaagtgcgtg gtgattacgg
      661 cggctataaa gatgattccg gcgtacatca cttgaacgtg ctggacttcc agcgtcagat
      721 ccgtacctgt ccgaaaccgg ttgtcgcgat ggtggctggc tactccatcg gcggtggtca
      781 cgttctgcac atgatgtgcg acctgactat cgcagcagat aatgccatct tcggtcagac
      841 tggcccgaaa gtcggttcct tcgacggcgg ctggggcgct tcctacatgg ctcgtatcgt
      901 cgggcagaaa aaagcgcgag aaatctggtt cctgtgccgt cagtacgacg caaaacaggc
      961 gctggatatg ggccttgtca acaccgtggt accgctggcg gatctggaaa aagaaaccgt
     1021 ccgttggtgc cgcgaaatgc tgcaaaacag cccgatggcg ctgcgctgcc tgaaagctgc
     1081 gctgaacgcc gactgtgacg ggcaggcggg gctgcaggag ctggcgggca acgccaccat
     1141 gctgttctac atgacggaag aaggtcagga aggtcgcaac gctttcaacc agaaacgtca
     1201 gcctgatttc agcaaattta aacggaatcc gtaatgcgta gcgcgcaggt ataccgctgg
     1261 cagatcccca tggacgcggg ggtggttctg cgcgacaggc ggttaaaaac ccgcgacggg
     1321 ctgtacgttt gcctgcgtga gggcgagcgc gaagggtggg gggagatctc cccactgccg
     1381 ggcttcagcc aggaaacctg ggaagaggcg caaagcgtgc tgcttgcctg ggtaaataac
     1441 tggctggcag gcgattgcga gctaccgcag atgccttccg ttgcctttgg cgtaagctgt
     1501 gcattggcgg agctggcaga tacgctcccg caggcggcca attaccgtgc ggcaccactg
     1561 tgtaatggcg atcctgacga tctgatcctc aaacttgctg atatgccagg cgagaaagtg
     1621 gcgaaggtca aagtgggatt gtacgaagcc gtgcgcgacg gcatggtggt taatctgttg
     1681 ctggaggcaa ttccggatct gcatttgcgt cttgacgcaa atcgcgcctg gacaccgctg
     1741 aaaggccagc agtttgccaa atatgttaac ccggattatc gccaccgcat cgcgtttctc
     1801 gaagagccgt gcaaaacccg cgatgattcg cgagcgtttg cccgtgaaac cggcattgcc
     1861 attgcctggg atgaaagctt gcgcgagccg gattttgcct ttgtggctga agagggcgtg
     1921 cgcgcggtag ttatcaaacc cacgctcacg ggcagtctgg agaaagtacg cgagcaggta
     1981 caggcggcgc acgcgctggg gctgacggcg gtgatcagtt cttccattga atcgagctta
     2041 ggcttaacgc aactggcgcg gattgccgcc tggttaacgc cggacaccat tccagggctg
     2101 gacacgctgg atctgatgca ggcgcagcag gtacgtcgct ggccgggtag cacgctgcct
     2161 gtcgtggaag ttgatgcact ggagcggttg ttatgatctt ctctgactgg ccgtggcgtc
     2221 actggcggca agtgcgggga gaagccatcg ccttacgtct taatgacgag caacttaact
     2281 ggcgcgagct ttgtgctcgc gtcgatgaat tagcctcagg ctttgcggcg cagggcgttg
     2341 ttgagggcag cggcgtgatg ttgcgggcgt ggaatacgcc gcaaacgctg ctcgcctggc
     2401 tggcgttact gcaatgcggg gcgcgggtgt tgcccgtgaa ccctcagctg ccgcaaccgt
     2461 tgcttgaaga attgctgccc aatctgacgc tgcaatttgc gctggtgccg gatggggaaa
     2521 acacgtttcc ggcgttaacg tcgctgcaca ttcagcgggt tgaaggcgca catgccgcta
     2581 cgtggcagcc gacgcgtctg tgctcaatga cgttgacctc gggttctacc gggttgccga
     2641 aagccgctgt ccatacttat caggcccatc ttgccagtgc cgaaggggtg ttatcgttga
     2701 ttccgtttgg cgatcacgat gactggttgc tctctttacc gctgtttcac gtctccggtc
     2761 agggaattat gtggcgctgg ttatacgctg gtgcgcggat gacggtgcgt gataaacagc
     2821 cattggagca aatgctggca ggctgtactc acgcttcact ggtgccaaca caactctggc
     2881 gtttgctggt taaccgcagt tccgtttccc tgaaagcggt gttacttggc ggcgcggcta
     2941 tcccggtcga gttgacggaa caggcgcgcg agcaggggat tcgttgcttt tgcggctatg
     3001 gtctgaccga gtttgcctcc acggtgtgtg cgaaagaagc cgacggcctg gcagacgttg
     3061 gttcgccgct gccgggtcgg gaagtgaaaa tcgttaataa tgaagtgtgg ctgcgggctg
     3121 ccagtatggc agaaggttac tggcgtaacg ggcaactggt ttcactggtt aatgacgaag
     3181 gctggtacgc tacgcgcgat cgcggtgaga tgcataatgg caagctgacc attgtcggac
     3241 gtttagacaa tctgttcttc agtggcggtg agggtattca gccggaagaa gtcgagcgcg
     3301 taattgctgc acatcctgcg gttttgcagg tgtttatcgt ccccgttgcc gacaaggagt
     3361 ttggtcatcg accggtggcg gtgatggagt atgaccacga gagcgttgat cttagtgaat
     3421 gggtgaaaga taagctggcc agttttcagc aaccggtgcg ttggttaacg ttgccgcctg
     3481 aactgaaaaa cggcggtatt aaaatttcac gtcaggcgct aaaagagtgg gtgcaacgtc
     3541 agcaataatt aaccctccgt ttcacttctc atttctgcca ttccattgcc gttgataaag
     3601 agtttatctg ttaatctgta aattgatgtg aaaactctta gcaaacagga taatgcaatg
     3661 gaatggctgg tcaaaaaatc gtgctgcaat aaacaagata acagacatgt acttatgctc
     3721 tgcgatgctg gcggcgcgat aaaaatgatc gccgaagtga agagtgactt cgccgtgaaa
     3781 gtgggggatt tactctcgcc tttgcagaat gcgctttatt gtattaatcg tgaaaagctg
     3841 catacggtga aagtcctctc ggcaagcagt tatagccccg atgagtggga acggcagtgt
     3901 aaagtggcag ggaaaactca gtaacgttgt tttgtcatgg gcaggaaaat cagcatcaac
     3961 ccgctcataa tacaggctac tcccagtagt gctttcagcg aaaaagtccc ttcccagccg
     4021 ggaagtacca ttgaagcaat ccataccagc acataactca tactgagcaa ggcataggct
     4081 ttgcttagcg caagtttatg tagcgtttta taccaacaaa aaacggaaag cagataaccc
     4141 agcagcccga gtaacagtat ccgggcatcc aggccaaaag ctaacagagc cgcgataaaa
     4201 tcccataagt gcgtcatcgg cggcagatga ctcgccgcaa aacccaggct taattgcgca
     4261 actgaggcaa taatgacgct gaataatccc cacatcaggc ccattacacc gtactcccga
     4321 ggatcacaat gccaccaata atgaacgcca ccccacacca gtgacgcggc gataccggtt
     4381 cgtgccacag ttttactgca gccagcgtca cccagacaaa attcagactt aacatcgggt
     4441 aggcaatgcc taccggtacg ttctgcaaga ccagcagcca gagcaccatg gcaagaccaa
     4501 gacaagccag cgccagtccc agccacagca cgatatgttt gcgccgtttg tttatcgcca
     4561 caaagcaggt tgcctgtttc tgacacaact gcccggcaac gctaagcaag ctggcaaaga
     4621 ctaatgttag ccagatcatt tgggacgata ctgaatcagc accagacgct cctgacgatc
     4681 gatggcatct gcgggcggaa tggcgagact gttgatatct tcatcgcggt caaccgaaag
     4741 cacgagagta ataatcccct cctgacgatg ttgattaagc cagtttgcga actcatcacc
     4801 gctgacaaat ctccctttcg catccggata attaaggccg tatttcaact cacctgtctg
     4861 gcgatacatg atgatgtcat cgcgttgcag gctccatgcc agacctgcgg caacaccgac
     4921 gctatcagta agaatatagc ggcttggctg aagtgattct tgtgtcatct cgacaaaaaa
     4981 ttgcggatgt tttccttcca taactctgtc aggaattgaa aatcctacca gcaacgccag
     5041 ccccagcggg caaagcgcgg caaaaggcca ggtcttttcg acgtttgtta aggtgtacca
     5101 gccgaaaaat gcccatagcg aaaaaatcga ccaggcacaa aagactttat agctctcgaa
     5161 ggtttgccac accggcgtgt tcattggtcc ccacggggag accacaaatg tggcaataat
     5221 gccagtgacg ccaaaagcga tgttaatcca gccattaatc cgcagcgcca gaggattatt
     5281 ttttgctgcc agcaaagcgt aatgcgccat cagcattgcc agagatgcaa agcaggaaag
     5341 aatataggtg ggcaatttac ctttagcgac ggagaaaaac agcagcggca ttatcgtcca
     5401 gctcaacaaa tagacggttg cggaatgctt gcggtttttc cagcctgtgt acagtgcacc
     5461 ggggagtaat cccagccacg gcaggctacc ggcaatgatg accggcacgt agtaccagaa
     5521 cggagctcta tgttgggcat catccagtgc aaagcgttga atatgctcaa cccagaaaaa
     5581 atagtgccag aagtcaggct cccgctgcgc tatcgccagt ccccaaggga gaaccgtcag
     5641 tacgcaactg ataaccgcca gccagccgta aataaagaga tctttccagc gtttttgcgt
     5701 cgctacccat ggcagcacgc ttaataccgg cacggcaagg gcgagaaaac ctttggtcat
     5761 cacccccatg ccgcaggtga ttcccagcag taaaaatcct gcgcttttgc ctttccacgt
     5821 ctgtgcctgc attgccagcc agaagctgca cattcccgcc accagccaga atgcaataaa
     5881 cggatcgagc acggcataag tgccgatggc atagacaata aacaatgaga gataaattac
     5941 tgtggcgagt agagccagac gtttatcgcg ccataagcgc agagtaaacc aggtcaccag
     6001 cgcggcagtt aacagggtcg caaagataac gcctgcccgc acaccaaagt tattcgcgcc
     6061 aaatagccat tgcccaatgc tgttaatcca gtatccggca atgggttttt cgaaataacg
     6121 tagccctaac agatggggca caatccagtc gccggatgcc agcatttctc gactgatttc
     6181 cgcataacgc gtttcatcgg gttgccagag cagacgcgtg ctgatcggta acaggtaata
     6241 gcaggcaata aacgcgaaga ggccgataag gtaacgtacc gatttcatca gcgactaccc
     6301 gcaatttgtt ggcaacccag ccagccttca cgtccggcaa tatttccgcg aacaacttgt
     6361 ccgagcggca acgtctctga taacagttcg cttaaagggc aaaatgtcac gccttcctga
     6421 gctgcgcgtt tgaggagatc cacaaaatta tgctgataag cgcagccttc aacttctgca
     6481 tgaatggtat aaaccggcgt gcctttatct cgcaggatgc ggttgagtaa ccaaccgtta
     6541 aaatcttctg ctttcacatc ccgaccaatc acttcatccc aggtgggtaa ggtcaccgga
     6601 atttgcgcag tgccagggtt tcctgattcg agcaatggac ggaacggcat ggccccacga
     6661 caatcgctgt tgtagcgcaa atggaacgct tcttttgctt cgatcacctt ctggtcggca
     6721 cgccagcccg cagcggcaga acaggttacc ggttgaccga taatctcttc cagagtgcga
     6781 agaccgcgtg caatatcgtc gatcattgtt tgccgatccc agttaccgct acgggcttgc
     6841 caggcatggt gatcccaggc gtgcaggccg acttcgtgat gtttagccgc ttcacgaatg
     6901 atatcggcat tggcatgacc aatctcttta cctggccagg ccgtacctgc cagtaaaata
     6961 tcccagccat aaagcgatgc cgcatttgag cgcaacatct tccacaaaaa ctgtggcttc
     7021 accagtcgcc agagatggcg gcccatattg tccgggccga cgctgaaaaa aatgctggcc
     7081 tgaatattat gcttactcaa gatttccagc agacgcggca cgccttcacg ggtgccacga
     7141 aaggtatcga catcaatgcg taagcctact ttggtcatga tggtttatcc gtaagatcaa
     7201 cggtgcgcag gaagaaatcc agcgtttcgt cgatggtttc ctgcatatca attttcggct
     7261 cccagtccag gcagcggtgg gcattgcgga tgctcggttt acgatgctct acgtcctgat
     7321 atcctttgcc gtagtagctg ctactttcga caacgcgaaa gcccgcaaac ggtgggaaat
     7381 gatggcgcag cggatgtttt tcgaagctcg ccagcagcat ctcgcccagt tcctcaatgc
     7441 tcgcttcgtt ctcaggattg ccaatgttga taatttcacc gtcgcagcga tttcccgcat
     7501 tttcgataat gcggtataac gcctcgatac catcgcgaat atcagtaaag cagcgttttt
     7561 gttttccgcc atcaatcagc ttaatcggtg aaccttctac cagattgaga atgagttgcg
     7621 taatagcgcg ggagctgccg attcgtgccg cattaaggtt atccagtcgc ggtcccatcc
     7681 agttaaacgg gcggaagagg gtgaactgta aaccctcttt ttcgccatag gcccagatca
     7741 cccgatcaag taattgtttt gataccgaat aaatccagcg tggtttattc accgggccga
     7801 cgattaaatt agaatggtcc tcatcgaagt atttatcgct acacatccca taaacttctg
     7861 aagttgacgg gaagatgatt cgcttacggt acttcacgca gtagcggata atgcgcagat
     7921 tctcttcaaa atcgagttca aatacgcgca gcgggttgcg ggtatattca atcggcgtgg
     7981 ctatcgccac cagcggcaag acgacatcac attttttgac atgatactca atccattcgg
     8041 aatgaatact gatatcgcct tcaacaaagt gaaaatgcgg atgattcaga aaacggctta
     8101 tcgcatcgct gccaatatcc agaccgtaaa cttcataatg atcttcgcgc agcaggcgtt
     8161 ctgtcagatg gttgccaata aagccattca ccccgaggat gagtacccgg gtacggcgtc
     8221 gggcggtgca ggcaggctgg ctattcaagc gtgaaccttg caccaggccc agcgtctgcg
     8281 ctaattgcga gccctgcata gtaatgccgt cgcccgcctg tccggtgacg atttccagcg
     8341 cgccatcgcc acaggcaatc agcagtggcg caacagaaat cacgctcccc ggctgtgctt
     8401 tgctggcatg aggatgaaca cgcgacgacc agacggtgaa tttctgattg ccaacatagc
     8461 tgaaggcacc cggccacgga tcggcaacgg cacgtaccat gttgtgcagt acggatgccg
     8521 gtttatgcca ttcaaggaaa ctgtcatccg gcgttctgcg accaaaacag gtggcttcgt
     8581 tttcgcgctg ggcgatttcc agaatattac cgtgtttaat ggcgggtaat gtctgttcca
     8641 gtagctggcg cgcggcatgg cacaatttat gatgcagcgt aatagcgata tcgtctggcg
     8701 caatggcaat gcgcagttgc gccacaatgg ccccggcatc ggcacgtttc accattcggt
     8761 gcaatgtaac gccagtttcc gtttcaccgt tgaccagcac ccagttcagc ggcgcgcgac
     8821 cacgatattt tggtaacagc gaaccatgca gattaaatgc acctgcggga gcgagctgca
     8881 aaatttcgtc gcaaataaga tggcgataat aaaaagagaa aatcacatct ggcgacagtt
     8941 gggcaatgcg ttccacccac agcggatgat taacgtcatc cggcgcataa accggaatgc
     9001 ctctttccgc tgccagacga gccaccgaac cataaaaggc tttttcaccg ggattatcgg
     9061 tatgggtaaa aatggcgcta atttcgtaac cggcagccag cagagcttca ataccgaggc
     9121 atcccatatc gtggtaggca aaaacgacgg ttttcattat tcattttcct tgctggatgg
     9181 acggataact tgctgaacaa aatagcgggg gcgggcgcgg acatcggtgt agatcctgcc
     9241 gatatattca ccgagtaatc ccatgccgat aaactgagcg ccaataaaag taaacagcac
     9301 ggcaaatagc ataaagacgc cttctgccgc ccattgtggt ccgaaggtca ggcgtaaaat
     9361 caccagcagc accgcaatgc taaaacctcc aatcgcaata atgctgccga gcagactcag
     9421 catacgtagc ggcgttgtgg taaggcaggt caccaggtcg tacatcaaat taatcaggcg
     9481 cataaaactg tatttggatt caccaaactc acgctcggaa tgatgtactg gaatttcaat
     9541 ggcacggcgg gcgaagatat tcgccagaat cgggataaag gtgctacgtt catggcagtg
     9601 caacatcgca tcgacaatat gacggcgata ggcgcgcagc atacaaccgt agtcacccat
     9661 cgctttgccg gtggtgcgct gaataagccg gttaatcatc ttcgaagctg ttttacgaaa
     9721 ccagctgtcc tggcggttct ggcgtacagt ccctaccacg tcgtaacctt catcggcttt
     9781 tgccaccagg cgggggattt cttctggcgg attctggaga tcggcatcaa gggtaataat
     9841 taagtcgccg gtaacgtgac tgaatcccgc cataatcgct gaatgttgcc cgtaattgcg
     9901 gttaagcaaa atagacacaa tatggctgtt ctccgcttgt gaggcttcga ccagcatatg
     9961 cgcggaatta tcgctactgc cgtcatcaat cagcaggatc tcatactctt tccccaacga
    10021 ttcacaggct gtggtggtgc gcctgattaa ttccggtaag ctttcctgct cgttataaac
    10081 gggaataacc accgagactt tcttaacagg gtggatttca aacatggctt attgtcctgc
    10141 gagttgctga agggctgtga tgacacggtc ggcatcggcg gtagtcatat ccgggaacag
    10201 cggcaaagaa cagatgcgtt cgctattcca ttcggtattc ggtaacgaca gcgtgggaaa
    10261 acgctcgcga taatattttt gtgtgtgagc ggcgcggaaa tgtaacccgg taccaatgcc
    10321 tctttctttt aacgcttcca tcaacgcatc gcgactgata ccacaacgtt gttcatcgac
    10381 acgaataata aacagatgcc aggcgtgaac gtgcggccag gcgggaaggc ttaatggctg
    10441 aaaggggaga gctgccagtg cttgctgata ttgctgggca atttcgcgcc gacgggtgtt
    10501 gaggtgctct aattttgcta actgtgtcag ggcaattgcg gcgttaatat cggtcagatt
    10561 gtacttatag cccggtgtta agacttcagc ctgcggtgca cggccccagg tttgtctgtc
    10621 ataggcatcg acacccagac cgtgaaattt cagcatccgt agctggcggg caaggttttc
    10681 attatcagtt acaatcaggc caccttccgc acaggtaata tttttaatgg catgaaatga
    10741 aaaaatagcg gtaccttttg cgccaatatg tcgccctttg taatacgtac cgacggcatg
    10801 ggcagcatct tcgataactg cgatgccgta acgttcgcca atggcgcgaa tggcgtcaat
    10861 atctgctggc gcaccggcat aatgcaccgg aatgatggct ttagtgcgtg gcgttatggc
    10921 tgactcgatc gcttcaggcg tgaccatcag cgtatcgcgg tcgacatcca ccattaccgg
    10981 cgttgcaccc aacaaggaaa tcatattgag ggttgaaacc caggtcaggg aaggcgtaat
    11041 cacttcatcg cccttgccaa tttccaacgc cattagcgtg atatgcattc cggcggtggc
    11101 tgaactgacc gcgatggcat gctgatttcc cgtcaactgg caaaaggctt gctcaagcgc
    11161 ctgattcttc ggaccggttg tgagccaacc ggattcgaga acctctttaa ctgcagcgag
    11221 ttcctccacg cccattgctg gtcgcgaaaa aggcaaaaat tctgacattg cttttccttc
    11281 cgccattgaa tacctgtcca cttatatttg ctataaagag tgttgtgtat attttgccat
    11341 ttggagcgaa attttaagga tagaatatta acttaacctt aagaaactaa tattagacgt
    11401 aaatattgaa atttttatat tttttcttat ttaggctttg catttggcaa aattttgagg
    11461 cattttgccg acatcgtagg atttttaata ttacaccaac tgctaattat cgccagaaat
    11521 gtcgctcaat tttacggtaa ttgtctggtt gcgcttgtct ataggtggag tttacgtgtt
    11581 agctttttgc cgctcatcat tgaagtcaaa aaaatatttc atcattttac tggcgctcgc
    11641 tgcaattgcc ggactgggta ctcatgccgc ctggagtagc aatggtttgc cacgtatcga
    11701 caataaaaca ttggccagac tggcacagca gcacccggtt gtcgttttgt ttcgccatgc
    11761 tgaacgttgc gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta ttacggttaa
    11821 aggtacccag gatgcccgtg aactgggcaa cgcttttagc gctgacatcc ctgatttcga
    11881 tctttattcc agtaataccg tccggaccat tcagtcggcg acctggtttt ccgcgggtaa
    11941 aaagctgacg gtagataaac gacttcttca gtgcggtaat gagatttata gtgcaattaa
    12001 ggacttacaa agcaaagcgc ctgataaaaa tatcgttatt ttcacccata atcattgcct
    12061 gacatatatt gctaaaaata agcgtgatgc gacatttaaa cctgattatc tggatggttt
    12121 agtcatgcat gtggaaaaag gcaaagttta tctggatggg gaattcgtta accactaaat
    12181 tttcaatctg acagccagtg atggctgtca tcattgttaa agaagacctt tcaaacgtaa
    12241 cgtttttcgg gcggcaacat ttaaatcata atgcaccaga tcttcaggtt ttacccacgc
    12301 gtagtcctga aactcttcgt ttattttcac ttctcggttg gcagaaacgc agtcaaaaat
    12361 caggtaaatc atataaatct cttccttgcg accatctgca tacgtcttgg tgcgaatatc
    12421 atcgctgaag gtccacggcg tgatttctgt caaaagcagc tgttctccca gttcttcgcg
    12481 aatttcgcgg cgtagtgcct cttcaattcg ttcgccagac tccacgccgc cacccgaaag
    12541 cgcccattga ccggggaaaa cgccgcgatc gtcggccatt ttacacagca aataagcacc
    12601 atcattttga atcaaagggc atacaatagt ccgttgtcgc ataggggtat tccttataaa
    12661 taaaaagaaa ccatgagaat acggtaactc agaaatatgt gaaagtgaca gtaatctctt
    12721 ttgcaatgat aactgcactt attattattt ttgtgtgtat cggtaaagtg tgtttatttt
    12781 ttcggcgttt aattatttaa gaagtattta gttacaataa gtgactagat cccttttttc
    12841 gctcattgaa tcctctgtgc caggcagtac aatcccgcag ctttgggaat tttgattaat
    12901 tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc ggaatgctgt
    12961 tggtttctgc atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa gaatacacca
    13021 atattggtgt cggttttggg actgaatcga cgggcctggc tttaagcggt aactggacac
    13081 ataacgacga cgacggtgac gtcgcgggcg tggggctggg gttgaatctg cctctcgggc
    13141 cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa cccgaattac ggcgatgaag
    13201 gttatgccgc agcggtagga ggtggtttgc agtggaaaat tggcaacagc ttccgtttgt
    13261 ttggcgagta ttactactct ccggattcgc tctccagcgg tattcaaagt tatgaggaag
    13321 cgaatgctgg cgcgcgttac accattatgc gtccagtcag tattgaggcg ggttatcgct
    13381 acctgaatct gtcgggtaaa ggcggtaacc gcgacaacgc cgtggctgac ggcccgtatg
    13441 ttggggttaa cgccagtttc tgatcccttc cggcgcggca ttctgtcgcg ccattcagtt
    13501 tcttactacg ccttatctcc ctttgcgtta tagtgattcg ataacaaaag caggagataa
    13561 ccatgttaaa agtggaaatg ctatccaccg gggatgaagt gttacacggg caaatcgttg
    13621 acactaacgc tgcctggctg gccgattttt tctttcatca ggggctgcca ttatctcgcc
    13681 gcaatacggt gggggataac cttgatgact tagtcaccat tcttcgcgaa cgtagtcagc
    13741 acgccgatgt gctgatcgtt aacggcgggc tgggaccgac cagcgatgat ttaagcgcac
    13801 tcgccgctgc gacagcaaaa ggtgaaggca tggtgctgca tgaagcctgg ctcaaagaga
    13861 tggaacgcta tttccacgaa cgtggacgag taatggcacc gagcaaccgt aaacaagcgg
    13921 agctgcctgc cagtgctgaa tttatcaata acccggtagg caccgcctgt ggttttgccg
    13981 tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt accgtcagaa tttaaggtga
    14041 tggtcgagca cgaaatcctg ccgcgtctgc gcgagcgttt ttctttacca cagccgccgg
    14101 tttgtctgcg tttaactacc tttggtcgtt cggaaagcga tctggcacaa agcctggaca
    14161 ctctacaact gccgccgggc gtaacaatgg gctatcgctc ttcaatgccg attatcgaac
    14221 tgaaactcac cggaccggca agcgagcaac aggcgatgga aaaactgtgg ctggacgtta
    14281 aacgtgttgc cggacagagc gtgattttcg aaggcaccga aggactgccc gcgcagatca
    14341 gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc accggcggtt
    14401 tattggcttt gcaactttct cgcgcaggtg ctccattgct ggcgtgtgaa gtggttcctt
    14461 cacaggaaga aaccctggcg caaactgcgc actggattac agaacggcgg gccaaccatt
    14521 ttgccgggct ggcactggct gtttcgggtt tcgagaacga gcatctcaac tttgcgctag
    14581 ccacgccaga cggcactttc gctctgcgtg tgcgtttcag cactacacgc tacagcctgg
    14641 ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat gctgcgccgt tggttaaacg
    14701 gccaggatat cgccagtgag catggctgga ttgaggttat tgagtccatg accttatctg
    14761 tctgattccg ccactctgtg ccggtttact gcaccggcac attccccctc cggcaaaact
    14821 tgtccaaccc tccctacggt gagcgtggtg ttggggccag tgtggcacgg gctgcgcgct
    14881 ggggacgcat tgagaattac atggcgcaag ttaacgattc actttgtctg ttggtgcagg
    14941 tggaaagtaa aacggcactg gataacctgg acgaaatcct cgacgtcgaa gggattgatg
    15001 gcgtgtttat tggacctgcg gatctttctg cgtcgttggg ctacccggat aacgcagggc
    15061 acccggaagt gcagcgaatt attgaaacca gtattcggcg gatccgtgct gcgggtaaag
    15121 cggctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg tggggagcga
    15181 actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat caacgactgg
    15241 cgatgtttaa atcaggcaaa aatgggccac gcataaaagg tagttattga tatcaaaggc
    15301 ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca gatcggcttc
    15361 gcttagctgg gtggccgcga gcacctggtc acgatcaatg ccctgttcca gcatgcgcaa
    15421 agcaatttta atggcttgtt catgcatacc ttcctgccag ccaatttgat gcccttcctg
    15481 ccgtagccgt tcagcaatag tcattaatct ctccttttgt agtggtgaac gttcggcaat
    15541 ctcctcaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat aattaaacag
    15601 tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga tcctgtcgac
    15661 catgccgatt aaatcgcggt cgcgaatatg cttttgaatc agttccagca gagctatacg
    15721 ccgatgttgc atgatctcat cgtcaggtac gatggtgata tccaccaacg gaaaagcttc
    15781 ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt taagtgagta
    15841 cgggtagggc gatgtttcgc catgataaaa cagcaatggc accaccagcg gaactctgtc
    15901 atagccttta tccaggtgac gctgcatggc ggcagtggca tagcgcatta gccgaaaagc
    15961 catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc cgtcaccgtc
    16021 gctggtttcc acggaccaca aaacatccga gtagtaagcg cgtaaacttt tttcaataaa
    16081 actagtgggt tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg gttctggtaa
    16141 atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt taaacaccgc
    16201 atcatgcggg gaggatgttg ttgattcggt catggttgtc cgtcacctga tatttatgat
    16261 gacgcgacat tacgtctatc ccgggccttt gacatctgtc agtttcagcc tttgcgagac
    16321 attttcagag gaattttcaa agcgttgcaa cgcatacatg gtgcgtggaa tcgcgctcgc
    16381 aaaaatgccc gataagcaat gtgcttaccg ggcatcaaaa ggtcggttaa gccagcgcct
    16441 gggccagtag cgtaatcgga tgttcgcagc gaaggctcgt ggacatctca atctgccatt
    16501 tgcaggtttc gcagtcggtg atgaccagat ccgcgccgct ctcttctatc tggcggaaca
    16561 gtggcgcacc gatggcttgt gaggtggggt agttctcttt tttgaaaccg taagtacccg
    16621 caataccgca gcactgggaa tccagcaccg ttaactcaag ccccgggatt ttacgcaaca
    16681 gctccagggt gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca
    16741 ctttcagcgg cagcggtttc agcggtaacg ttttgccttc gtccagcttg cgccacagcc
    16801 agcgggttgc cagttcgata tgatcgcgca agcctttgtt gtcgacattc agcacttccg
    16861 ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt
    16921 ttactcccac agcttcgcgg atcgactcta cattcgtaat tgcctgtttg cgtgctttat
    16981 cggtaaagcc gttggcgatt agcggtacgc cgcagcattt ttctttgctg agcagttgta
    17041 caccggtacc cattgcgttg agcactttaa ttaaatcttt acctaactgc ggatggttgt
    17101 agttaacgaa gcagccgtga aagaaagcga cctggtcttt atattgtgct tgctgagccg
    17161 ctatgctgcg ataccagcga cggaacgtgc cgaaggagta tttcggtagc gtgcggcgat
    17221 gatcgatttt taacgccgca tcaagcatct gccgcaccgg tttcagcgag gtagcggtgt
    17281 tgacgattgg tgcgaacggc gtggaaacgc tacccatcag gtcggtatga ctcaacacaa
    17341 aattacgcag cgacgggcgc gtggtgtcat attttgcccg cgcgcgctgg ataatatcgc
    17401 caatcttcac atcggacggg caggcgactt cacaacgttt gcagttgatg caatatttca
    17461 gcgcctcgtc atacagtgcg ccatctttca aacgcagacg ctcgccatcc ggcccggctt
    17521 gttttggccc tggataaccg ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg
    17581 tgcacttaat gcagttttcg aagctggtgt cgttcattgt tggcctcctg cgcgttgggc
    17641 aatctgttga gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccctgggc
    17701 gatgggatca aatccgccca gcaccgaacc gatggcaaac aggttctcaa tggtttgccc
    17761 tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg aactgctgcc acggttgcgg
    17821 cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc
    17881 gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt ccgccactaa agaaactgcc
    17941 gctggcgaga accgcgaaac gtggacgtag cggaatatcg gcgtgattgc gggtccagat
    18001 ttcattcact acgccatttt tacaggtcac ttttttcact tcatcgcccg gcatccacac
    18061 gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga
    18121 aggcggcagc gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca
    18181 cagtttgtca tcggccaggc cgaagcaggc gggcatcagg atcatttcgc aggtattggc
    18241 gacaggaata agcgcatcaa gtaacagcgg ccagttttct tcattatcaa ggaaacgggc
    18301 gatattcaca gcgcgaaatt cggtggcgtt attgcgcagg acatccagtt ccggcagctc
    18361 tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac gaagccgccg caagatgcgc
    18421 ctggaaatcc atcagcccgc ttatccccac tacacatatc ttcttcgcgg gtagcggcca
    18481 gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa gtgccgagcg gggtaacgcg
    18541 ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg ctttcagcga tcaatgcctg
    18601 cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga agactgtaag gatgggctgg
    18661 tgcctgctga cgcaaagatt ccagtccact gtgaatgtct gtcaccggtt gaccatcagg
    18721 cagatggctc agcaaatcca gcgaaccgga tgagaaatgc agcgcgcttt gaccacgcgt
    18781 gacaatggca cagcgcaggc cgtgtttttg cagttgcagg ccacagagta atccggcgag
    18841 gccgccgccc ataatgacag tatcaaagcg catctttctg ctccttctcc agaccacaca
    18901 gtccctgata aacccagcgg gtaaattcgc tttcgcgcag tgcgtctccc caggcgatgg
    18961 gttgcacgcc tttccagcgt tcgttaagga aggtggaaag ttgctcgata gattgcgcgg
    19021 acgtcgtgac gttaaaacgt tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct
    19081 ggcaggtacc catccccaca cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta
    19141 aattttctac cgcgtactga acttcacccg cagttaccgc ttcgcactca cataccaggc
    19201 tacgatgcag acggccttcg ctcagccagg caggcgtgcg atcgccatga cgataaaccg
    19261 cagaaccgcg cagcggggca ggcagggaga tgactttacg caaggtaact tcagcgggtt
    19321 cttgtgaacc aggcagtgcc agatcggcag tcgtacaggg gcgcgtgttg cccagtttgc
    19381 ggcataccgc gtcggtagcc cattcagcca tcagccgata ggtcatcagt ttgccaccgg
    19441 tgatggtgat aaatccgtcc agaccatcgc gttcagcatg gtcgagcagc acgatgccac
    19501 ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac cagcgggcgc acgccagaat
    19561 aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag tttttcccct tcacgcagca
    19621 gaatatcaac ctcttctgcc gccactcgat tatcgtcaat ctcgttgtaa tcaatacgta
    19681 aagaggtggt gccaatcagc gaaatggtat cgccaggcac cagaatatcg gcgtcggaag
    19741 gtttacggca gcggttgatc acatgctggt taatgcggtg atccatgatc agcagcgatc
    19801 ctttcgccgg gaacatgcga atgcgcagat cggcatattc ggcaatgtgt tgcccccaga
    19861 tcccagcggc attaaccacg acaggtgcat gaagggcctg agtttcgccg gtgagatggt
    19921 tacgtacacg aacaccgcac accgtcgcgc cttcacgaat cagccccgtg acttcatgag
    19981 cggtaaggat aacggcaccg tgttctttgg catccagcat gtttgctgcg gtcagacgaa
    20041 atggatcaac ggtgccatcc ggaactttca ccgcgccaat cagtgccggg ttaacggcag
    20101 gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct gcttcttcgc
    20161 aggcgcgaat aaaagtggcc tggaatgaga ggtcatcttc cggcagggtg ataaacaggc
    20221 cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt tcactaatgc
    20281 attcgcgggc cgattccgca tcggttaccg catagcgcgc accgctgtgc agcaggccgt
    20341 ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc
    20401 cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc gccgccgcca atgataatca
    20461 cgtcacttga ttgcgagtcg cgagttttca ttgttatccc tctgaagttc gttttttacc
    20521 atttagccat agtaaaaaca tgaattgttt gatttcgcgc atattcgctc ataattcgaa
    20581 agtgaaacgt gatttcatgc gtcattttga acattttgta aatcttattt aataatgtgt
    20641 gcggcaattc acatttaatt tatgaatgtt ttcttaacat cgcggcaact caagaaacgg
    20701 caggttctct cactgaatca ggctgttaat cataaataag accacgggcc acggaggcta
    20761 tcaatgttga gtatttttaa accagcgcca cacaaagcgc gcttacctgc cgcggagatc
    20821 gatccgactt atcgtcgatt gcgctggcaa attttcctgg ggatattctt tggctatgcg
    20881 gcttactatt tggttcgtaa gaactttgcg cttgctatgc cttatctggt tgagcaggga
    20941 ttctcacgcg gtgatttagg ttttgccctt tcggggatct cgattgctta tggattttcg
    21001 aaattcatca tgggttcggt atcggatcgc tcgaatccgc gcgttttcct gcccgcaggt
    21061 ttgattctgg cggcggcagt gatgttgttt atgggctttg tgccatgggc gacgtcgagc
    21121 attgcggtga tgtttgtact gttgttcctc tgcggttggt tccaggggat ggggtggccg
    21181 ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag aacgtggcgg cattgtgtca
    21241 gtgtggaact gtgcgcacaa cgtcggtggt ggtattccgc cgctgctgtt cctgctgggg
    21301 atggcctggt tcaatgactg gcatgcggcg ctctatatgc ccgctttctg cgccattctg
    21361 gtggcattat tcgcctttgc gatgatgcgc gataccccgc aatcttgtgg tttgccgccg
    21421 atcgaagagt acaaaaatga ttacccggac gactataacg aaaaagcgga acaggaactg
    21481 acggcgaagc agatcttcat gcagtacgtg ttgccgaaca aattgctgtg gtatatcgcc
    21541 atcgccaacg tgttcgttta tctgctgcgt tacggcatcc tcgactggtc accgacttac
    21601 ctgaaagagg ttaaacattt cgcgctggat aaatcttcct gggcctactt cttctatgag
    21661 tatgcgggta ttccgggcac cctgctgtgc ggctggatgt cggataaagt cttccgtggc
    21721 aaccgtgggg caaccggcgt tttctttatg acactggtga ccatcgcgac tatcgtttac
    21781 tggatgaacc cggcaggtaa cccaaccgtc gatatgattt gtatgattgt tatcggcttc
    21841 ctgatctacg gtcctgtgat gctgatcggt ctgcatgcgc tggaactggc accgaaaaaa
    21901 gcggcaggta cggcagcggg ctttaccggg ctgtttggtt acctgggcgg ttcggtggcg
    21961 gcgagcgcga ttgttggcta caccgtggac ttcttcggct gggatggcgg ctttatggta
    22021 atgattggcg gcagcattct ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa
    22081 cgtcgccatg aacaattact gcaaaaacgc aacggaggct aatggcatga aattgaagct
    22141 gaaaaacctt agcatggcga tcatgatgag cactatagtc atgggaagca gtgcaatggc
    22201 ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt gccagtggat atttgccgga
    22261 gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga gcggattatc tggaacagga
    22321 tttggtgatg accaaagacg accatctggt tgttctgcat gaccattatc tcgatcgtgt
    22381 tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat
    22441 agatttcacg ctggatgaaa ttaagtcgct gaaatttacc gaaggtttcg atattgaaaa
    22501 cggtaaaaaa gtacagactt atccggggcg tttcccaatg ggtaagtccg acttccgggt
    22561 gcacaccttt gaagaagaga ttgaatttgt tcaggggtta aatcactcta ccgggaaaaa
    22621 tatcggtatc tatccagaaa ttaaagcgcc gtggttccat catcaggaag ggaaggatat
    22681 tgcggcaaaa acgctggaag tgctgaagaa atatggttac accggtaagg acgacaaagt
    22741 ttatttgcaa tgttttgatg ctgatgagct gaagcgtatt aagaatgagc tggaacccaa
    22801 aatgggcatg gatctcaatc tggtacagct gattgcctat accgactgga atgaaacgca
    22861 gcagaaacag ccggatggaa gctgggttaa ttacaactac gactggatgt ttaagccggg
    22921 tgctatgaaa caggtggcgg aatatgccga cggtatcggg ccggattacc atatgttgat
    22981 tgaggaaaca tcgcagccgg gtaatatcaa actcactggc atggtgcaag atgctcagca
    23041 gaataaactg gtagtgcatc cttataccgt gcggtcagat aaactgcctg aatacactac
    23101 tgatgtgaat cagttatatg atgctctgta taacaaagcg ggtgtaaatg ggctgtttac
    23161 tgatttccct gataaggcag taaaattcct taataaagag taatgctact gcaggatggc
    23221 gttcatgcgc catcctgtct cacatcaaat ttttccattc accacgagtt gttccgtaca
    23281 cgtttgtagt aaaaacagtg gactgccttc taccggcttt tctttagcca acaacgaaat
    23341 ctgtcgatag aaggttggtt cgtgaggaac ggaacacaat tcacctgcaa tgcttttcag
    23401 cgttaactct ggttgtaagg cgatgcctaa tccctgtcga ataaacttca tcgctgtatc
    23461 cgactgatta aattcacatt ttatctactg ggcgcactat tagtggtagt ggctggtgga
    23521 atctatgcgc tgcaaatgaa gggtaaaagc ggtgtcacgc tagtagtggc aaaagaaatt
    23581 cattaatacg acacgtttca ttaagatttt cctcaggtaa ccacctatag tcattgtcgt
    23641 taataaaaat gacaggcgat agggtatggc agtttcagca aagtacgacg aatttaatca
    23701 ctggtgggca acagagggcg actgggttga agagcctaac tatcgccgta atggtatgag
    23761 cggggtgcaa tgcgtcgagc gcaacggcaa aaagctgtat gtaaagcgca tgacgcatca
    23821 tctgtttcat tccgtacgtt atccgttcgg ccgaccaacg attgtccgtg aggttgcggt
    23881 aattaaagaa ctggaacggg ccggtgtcat cgtgccgaag atcgtttttg gtgaagcggt
    23941 gaaaattgag ggtgaatggc gagcgttgct ggtgactgaa gatatggcgg ggttcatcag
    24001 cattgctgac tggtatgccc agcatgcagt atcgccttat tctgacgaag tacggcaagc
    24061 catgttgaaa gcagtggcgc tggcgtttaa gaaaatgcat agcattaatc gtcagcatgg
    24121 ctgttgttat gttcgccata tctatgtgaa aacagaaggt aatgcagaag ctggttttct
    24181 tgacctggag aaaagtcgtc gccgcttgcg tcgcgataaa gcgattaatc atgacttccg
    24241 ccagctggaa aaatatctgg agccaatccc caaagcggac tgggagcagg tgaaagcgta
    24301 ttactacgcc atgtaatcgt tgtaggccag ataagacgca taagcgttgt atctggcact
    24361 acaaccaatt cacatctcga tttcaatatc gccttttgcc cggcaacaac agggcaaaat
    24421 ttcccccggc tgaataaagg ctaacggttc ggcaatccag tcaacctgac ctgcaaccag
    24481 acgggtgcga caggagccgc agtaaccttc gcgacactga tactcaaccg ccacattgtg
    24541 ggattccagc gccgccagaa gggaagggtg ttcatcctgg cacagcagtt gtgtgccagt
    24601 gatgcgcagg gtaacgcggg ccatcagagt tggaagttac tcaaatcgtc ggtgtccact
    24661 tccgcgtcaa tctgcccgac cagataagaa ctgacttcca cttcctgcgg agcaacctgc
    24721 acgttatcag acaccagcca ggtgttgatc catgggatcg ggttggagcg cgtctggaac
    24781 ggcagatcca aaccgactgc ctgcatacgg atattggtga tgtattcaac gtactggcag
    24841 agaatgtctt tattcagacc aatcatcgaa ccgtcgcgga acagataatc cgcccagtct
    24901 ttctcctgtt gagctgcctg aacaaacagg tcatagcact cctgcttaca ctcttcggca
    24961 atttccgcca tctcaggatc gtccgcgccg ctgcgcagca gattcagcat atgctgggtg
    25021 ccggtcaggt gcagggcttc gtcgcgggca atcaggcgaa taattttggc gttgccttcc
    25081 atcaattcgc gttctgcaaa tgcgaaggaa caagcaaagc tgacgtagaa acgaatcgct
    25141 tccagcgcgt taacgctcat caggcagaga tacagttttt tcttcagctc gcgcaggcta
    25201 acggtcacag ttttaccgtt aacggtgtgg gtaccttcgc ccagcagatg ccagtagctg
    25261 gtcatttcga tcagctcatc gtaatagctg gagatccctt ccgcacgttt ctggatctgc
    25321 tcgttggtga cgatatcgtc aaacacaaca gacggatcgt taacgatatt acgaatgata
    25381 tgagtatagg aacgggaatg aatcgtttct gagaacgccc aggtttcgac ccaggtctcc
    25441 agttccggga tcgagatcag cggcagcagt gccacgttcg ggctacggcc ctgaatggaa
    25501 tccagcagcg tctgatattt caggttgctg ataaagatgt gtttttcgtg ttcaggcagt
    25561 gcctggtaat cgatacggtc gcgagaaacg tcaacttctt ccggacgcca gaagaaagag
    25621 agctgctttt cgatcagctt ttcgaagatg tcatattttt gctgatcgta gcgagccacg
    25681 ttgaccggct gaccaaagaa catcggttct ttgagctgat catttttcgt ctgtgaaaag
    25741 gtggtatatg ccatgagtgt gtcctgttgg gagtttaatg ccggataagg cattttacgc
    25801 cgcatcctgt gcctgatgcg acgctgacgc gtcttatcag gcctacaaat ccgagccgta
    25861 ggccggataa ggcgttcacg ctgcatccgg catctcaata tcagatctta catgcgccgc
    25921 tttcgcagcc atcgtcctgg attgacggta ccagatcgtc ctgcgcgtct tcagcgccgt
    25981 cacgggtgtt ctgataatac agcgttttga ccccgaattt gtaggcggtg agcaggtctt
    26041 tcagcaactg ctgcatcggc acttttcctg acgggaaacg tgacggatcg tagttggtgt
    26101 tagcagagat cgactgatcg ataaatttct gcatgatacc taccagttgc aggtagccat
    26161 cgttacccgg catttcccac agcagctcat aggcgtcgtg caggtgctcg tagtccggca
    26221 ccacctggcg caaaataccg tctttcgacg ctttgatgct gacgtaaccg cgcggcggtt
    26281 caataccgtt agtggcgtta gagatctgtg aagaagtctc tgacggcatc agggcagaaa
    26341 gcgtggagtt acgcaggccg tgcgttttga ttgattcacg caatgcttcc cagtcgtaat
    26401 gcagtggctc attagcgatg gtatccagat ctttcttata ggtatcgatc ggcaggatcc
    26461 ctttcgcgta agtggtttcg ttaaaccacg ggcacgcgcc ttgctctttt gccagctcgt
    26521 tagacgcttt cagcaggtaa tactgaatcg cttcgaaggt tttatgcgtc aggttgttgg
    26581 cgctgccgtc ggagtaacgc acaccgtgct tcgccaggta gtaagcgaag ttgatcacac
    26641 caatacccag cgtacgacga cccatcgctc cacgtttggc ggccgggatc gggtaatcct
    26701 gataatccag cagcgcgtca agtgcacgaa ccgccagaat tgccagctct tccagttcat
    26761 ccaggctatt aattgcgccc aggttgaaag cagacagcgt acacagcgcg atttcaccgt
    26821 tctcgtcgtt gacgtcgttc agcggtttgg tcggcagggc tatctccagg cacaggttag
    26881 actgacgcac tggcgcgatg gccggatcaa acgggctatg ggtattgcag tggtcaacgt
    26941 tctgaatata gatacgaccg gtagacgcac gttcctgcat catcagcgag aacagctcaa
    27001 cggctttcac acgctgcttg cggatgctgt cgtctttctc atatttggta tacaggcgtt
    27061 caaactcttc ctgatcggcg aagaatgcgt cgtacagccc cggtacgtcg gacgggctga
    27121 acagggtgat atcttcacct ttcagcaggc gggtatacat cagtttgttg atctgtaccc
    27181 cgtagtccat atggcgcacg cggttgcctt ccacaccacg gttgtttttc aacaccagca
    27241 ggctttccac ttccagatgc cacatcgggt agaacagcgt tgccgcaccg ccgcgcacgc
    27301 cgccctgaga gcaggatttc accgctgtct ggaaatgttt gtagaacgga atacagccgg
    27361 tatggaacgc ttcaccaccg cgaatcgggc tacccagcgc acgaatacgc ccggcgttga
    27421 tgccgatccc ggcacgctgg gaaacgtatt tcacaatcgc gctggaggta gcgttgatgg
    27481 aatccagact gtcaccgcac tcgatcagta cgcaggagct gaactgacga gtcggggtac
    27541 gcacgccgga catgattggc gtcggcagcg aaattttaaa tgtggaaacc gcgtcgtaaa
    27601 aacgcttcac atattgcagg cgcgtttcac gcgggtagtt cgagaacagg cacgcggcaa
    27661 ccagaatata aaggaactgg gcgctttcgt agatttcgcc ggtcacgcgg ttctgtacca
    27721 gatatttgcc ttccagctgc ttaacggcag cataagagaa ggtcatatca cggtcgtgat
    27781 cgataaacgt gtccatctgc ttgaactctt cttccgtgta gtcttccagc agatgattat
    27841 cgtatttgcc catctcgacc attttcacca cgtggtcgta cagcgcaggc ggctcaaact
    27901 ggccgtaggc ttttttacgc aggtggaaga tcgccaggcg cgcggcgaga tactgataat
    27961 ccggcgcatc acgggagatc aggtctgcgg ccgccttgat aatggtttcg tggatgtcag
    28021 aggtcttgat accgtcataa aactgaatgt gggagcgcag ctcgacctgg gaaatcgaaa
    28081 cgttatgcag tccttctgcc gcccaatcca gaacgcgatg gattttgtcg agattgatgc
    28141 gctctgtgct accgtcgcgc tttgtcacca gcagattctg attcatgtat gtcgtacctg
    28201 tttttggaat tctttccgca atagtttcat gcagactacc tgtagtaagg gaagctgctt
    28261 agaacactat atataggggg tatgtttggg attcactgca agatagtgtg aaaatggccc
    28321 tcttgcaagt gcataacttt gtggataact caggaaggaa aaagtggctt tcgcgcacct
    28381 taggtcagac aaggtgtcca ggaaagtcaa tgggaagaga aaaatttgtt aaaaataact
    28441 gttcggcgat ttcttgaacg gcagaatagg ttggataaat tgatatagat ggtttattaa
    28501 tctttttgct aatgattgca aaaattgatc taagcacgag tcatcacata atctgatgta
    28561 tcagttgtgg tcaatatata aacgaatgta agcgattgtt gatgtcgaaa tggttgtttt
    28621 ctattcggtt attgttaaat gcgaagccat tttgttaatt taccttatat tataagaaaa
    28681 ttattcatta ctttaattta gtttaaatat aactggtatc ccttaaggtt attaacttga
    28741 attatgacgc actgatacta ttcatcaaat aataacaaaa tagccattgc atcgggttgg
    28801 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
    28861 taaaatgatg gttagatgaa tatgcggatt atctttctac gcaaggagta tttatctcta
    28921 ctcccgtcaa tgattgcatc tcttttctct gctaacggtt tcgcggcggc cattgattta
    28981 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
    29041 acgcaggact ggagtgtcgc cgatgggcaa tggctggttt tttcggatat gaccaataac
    29101 gccagcggag gagccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat
    29161 gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg
    29221 gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttctggtaac
    29281 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaatgatac tggtgccgtc
    29341 gatttacgtg tcactaacgc catgtttcgc aataatatcg ctaatgatgg caaaggtggc
    29401 gcaatttata ccattaataa tgacgtctat ttaagtgatg ttatttttga taacaaccag
    29461 gcatatacat caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat
    29521 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat
    29581 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
    29641 attgatattt ctgttgatga cagctacagc cagaacggcg gcgtgttagt ggatgagaac
    29701 aacagcgcag caggctatgg agatggtcct tccactgcgg cgggtggctt tatgtatctc
    29761 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
    29821 gagaatgacg gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc
    29881 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
    29941 ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
    30001 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
    30061 gcagagctaa acgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag
    30121 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
    30181 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctgtcg
    30241 ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg
    30301 ctggaaggcg acgcggcaga tctcgccgct ctccaggacg atccgcagtc gatcgtgtta
    30361 aacggcggtg tgctcgatct ctctgatttc tccacctggc agagcggcac atcatacaat
    30421 gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
    30481 cttgcaggtg gcgacaattt gcatatcggc ggcgacggga aagatggcgt ctacgtggtg
    30541 gtcgatgcga gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca
    30601 caaatcgcct ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccactat
    30661 aaccgccagg ttatctttac cgataagcaa caaggaagcg tgatggagat tactgccaat
    30721 gtcgatactc gctctacgac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
    30781 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
    30841 gggcagcatc aggatgaggg tagcacattt actaaaacgg gggcgggtac gctggagctg
    30901 accgccagcg gtacaacgca gtcggcagta cgtgtcgaag aaggcaccct gaaaggcgat
    30961 gttgcggata tctttcctta tgcttcgtca ctgtgggtcg gtgatggggc gacgttcgtt
    31021 actggcgcgg atcaggatat tcagtctatc gatgctactt ccagcggcac tatcgatatc
    31081 agcgatggta cggttttgcg tctgaccggg caggatactt ccgtcgccct taatgcctca
    31141 ttgtttaacg gcgatgggac gctggtgaat gctaccgatg gcgtgacgtt gacaggtgag
    31201 cttaatacca accttgaaac tgacagcctg acctatcttg ccgacgtgac ggttaatggt
    31261 gatctgacca atacgtccgg tgcggttagc ctgaaaaatg gcgtcgctgg cgatacgctg
    31321 acggtaaacg gtgattatac cggcggcggt acgctactgt tcgacagcga attaaacggc
    31381 gatgactcgg taagcgatca attggtgatg aacggtaata ctgctggcaa cacaactgtg
    31441 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
    31501 ttcgcagctg atcccacgca gtttcaaaac aatgcacagt tcagtctggc tggcagtggc
    31561 tacgtcaaca tgggagcgta tgactacacg ctagtggaag ataacaacga ctggtatctg
    31621 cgatcgcaag aagtaacgcc gccatcgcca cctgatccag acccggaccc aacacccgat
    31681 cctgacccaa cacccgatcc aatccccgct taccagccgg tgctgaatgc caaagttggc
    31741 ggttatctca ataatctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcat
    31801 gcaggtggca atggtcagac gctgaattta cgtgttatcg gcggacgtta tcattacacg
    31861 gcggcagggc aactggctca gcatgaagac acttctacgg tgcagcttag cggtgacctg
    31921 tttagcgggc gctggggcga tgatggcgag tggatgcttg gggcggttgg tggctacagt
    31981 gataatcagg gcgagagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac
    32041 ggttatgccg ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa gcagggggcc
    32101 tggctggata gctggctgca atacgcgtgg tttaacaatg atgtttccga acaagacgat
    32161 ggcacagatc attaccactc gtcggggatt atcgcctcgc tggaagccgg gtatcagtgg
    32221 ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag
    32281 caggatgatt ttaccgccgc taaccatgcg cgcgtgtcac aatcgcaggg ggatgatatt
    32341 cagacgcggc tgggtttaca cagcgaatgg cgtaccgcgg ttggtgtcac accaacatta
    32401 gatctgaatt attatcacga tccccatgcg acggaaattg aagaagatgg cagcactatc
    32461 agtgacgatg cggtgaagcg acggggtgaa ataaaagtgg gagtcacggg caatatcagt
    32521 cagcgagtgt ccctgcgctg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag
    32581 acggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg gcacggttgc
    32641 cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc tgataagacg
    32701 cggcaagcgt cgcatcaggc attggctcgg ccaaacaaca acctcactta ttctgcgtgt
    32761 gcagcatata gttcacatcc acgccggggc cgagtttaaa agtattagtg atcgggttgt
    32821 aatgtagccc ggttatatgt cgctctttaa ggcttgtctg atctacccag cccagcaatt
    32881 ctgccggttt aataaacttc ttcacatcat gcgtgccttt gggcaccatg cgcaaaatat
    32941 attccgcacc aaccaccgcc atcagccatg acttgccgtt gcggttaagt gtcgagaaaa
    33001 agacatcgcc gcccggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg
    33061 ggacgtgctc cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg
    33121 cgtgctcttc cacggtttcc tgcacgtaat ccacctgaat gccgctttcc agtgcgtgca
    33181 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc
    33241 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
    33301 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcagcg
    33361 gtttgaactc accttccaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
    33421 cttcgtggtc tacgttatgg tttaccggcg atttttcggc attcattggc acttctactc
    33481 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
    33541 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
    33601 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
    33661 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
    33721 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
    33781 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
    33841 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
    33901 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
    33961 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
    34021 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc
    34081 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
    34141 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
    34201 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
    34261 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
    34321 cgcggcaagg tgtatatccg cgctcgcgca gaagtggaag ttgacgccaa aaccggtcgt
    34381 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
    34441 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
    34501 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
    34561 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
    34621 ttgcaccatg gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt
    34681 caccgccgtg aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
    34741 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
    34801 atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgc tggttgctaa tccgtggcag
    34861 ctgggcaacg ttgccgcgat gctcgaacgt gctggcgacg atgctgcgcg tccggaatgg
    34921 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
    34981 gcgattctgg atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac
    35041 gaatacaaag agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat
    35101 cgtttgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
    35161 cgtcgtactg aaatcaccgc caacagcgca gacatcaacc tggaagatct gatcactcag
    35221 gaagatgtgg tagtgacgct ctctcaccag ggctacgtta agtatcagcc gctttctgaa
    35281 tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
    35341 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
    35401 ggtcgcgtct attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt
    35461 cgtccgatcg tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca
    35521 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag
    35581 aaaactgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
    35641 gttgacggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
    35701 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
    35761 aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc
    35821 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc
    35881 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc cattaaggtt
    35941 actgaacgta atggtttagt ggttggcgcg gtacaggtag atgactgcga ccagatcatg
    36001 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtaggc
    36061 cgtaacaccc agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg
    36121 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgatgg cagtgccgcg
    36181 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
    36241 ttttacttct tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct
    36301 gcaaagtctg ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa
    36361 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
    36421 gttctatggt cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt
    36481 gcaatatgct ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct
    36541 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
    36601 caaagatctc ctcaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
    36661 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgtcgta ttgtctatga
    36721 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc
    36781 agcgctgtgg cacgataaac aaggccatct ttcacattat atggtgctga tccagcgcag
    36841 tggtttaagc aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag
    36901 caaaacggaa atcagtagca tcaagataaa tagtgaaact gttcctgttt atcagttgcg
    36961 ctataacggc aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc
    37021 cagcacggat atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg
    37081 tgatttgttg agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc
    37141 tgaaaaaacg ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta
    37201 ccagcggtta atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca
    37261 cagttttgtg gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc
    37321 ggtctggaac agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg
    37381 tattgccgaa gagatgcttt cgcacatcag tcaggaaaac gacaagttga atggggcgtt
    37441 agacggtgcc gcggggctgt gctggtatgc agactcaaaa ttgcaaaccc cgctgtttgt
    37501 cggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
    37561 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca
    37621 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc
    37681 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat
    37741 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac
    37801 actgaataaa aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
    37861 ctatatcaat ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc
    37921 gaagaatctc gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
    37981 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
    38041 ctggcagtgg ctacccataa cctggcagcc gctatgaggc acgggctgct ggcgctgatt
    38101 tgctggctgt gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg
    38161 tttcgcgcct ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc
    38221 tggtatcagc aggattgtgc gggcctggtg cgatttgcgg cgaacgagac gctgaaagtt
    38281 cacgatagta aatggctaaa aagtaacggt ttctctagcc agtatttgcc gccagagatg
    38341 acgctaacac ctggacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc
    38401 ggcccctacg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
    38461 ataaaccagg cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
    38521 atggtctgga tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac
    38581 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc
    38641 aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag
    38701 cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt
    38761 ccagtaacta tgcgccgccc gccgggggaa cattcttttt gcttgctgac agcagtttta
    38821 gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc
    38881 agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat
    38941 ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ctgggcgacg
    39001 ggctgaacaa tacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtaa
    39061 tgcagcgtac tttctcttct cagtcacggc agaatgtgac tcaggcatta cccgaattac
    39121 atctcggcaa tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttccccgc
    39181 tgaaaaaata tcccttggtg gaacagttcc gttatccact atggcaggct aaaccggtcg
    39241 agccgcagca aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg
    39301 gtaacattta tattcctctc ggccaacaag agccgggact gtacctcgtc gaggcgatgg
    39361 ttggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag
    39421 tgtcaggcaa cgagcttctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg
    39481 gctcagagat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca
    39541 gcggtacctt gcagttacaa catatatcgc cagaacgttc atacattctg ggtaaggatg
    39601 ctgaaggcgg tgtttttgtc tccgagaact tcttctacga aagcgaaatc tacaacaccc
    39661 gcttgtatat ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag
    39721 tgatgggccg cgagttccac gatccgttgc attcatcccc catcgtcagc gccccggcga
    39781 agctttcggt gctggacgct aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg
    39841 cgcgcaatgg cgggcaggga agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg
    39901 agttacgtct tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg gcaaactaca
    39961 tcaagccaca tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa actggcgaag
    40021 cggtcagcgg caaactgcaa ctgctctacc cggatggcga gccggtaaaa aatgcccgcg
    40081 tgcagttaag tttgcgcgct cagcaattat caatggtcgg taacgatttg cgttatgccg
    40141 gacgtttccc cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagc ggtcatgtgg
    40201 cgttaaatct ccccgccgcc gataaaccga gccgttattt gttaaccgtc tccgccagtg
    40261 acggcgcggc gtatcgtgtc accaccacca aagagatcct cattgaacgc ggtctggcgc
    40321 attactcatt aagtactgcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt
    40381 atgccgcgct ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag
    40441 accgcacgag ccatagcgga gagctaccgt caggcggcaa atcctttacc gtcaatttcg
    40501 ctaaacctgg caactacaat ctgacattac gcgataaaga cggcttaatt ctcgctgggt
    40561 taagtcatgc cgtcagcggt aagggcagca cggcgcatac tggtacggta gatatcgtgg
    40621 cggataaaac gctgtaccag ccaggcgaaa ctgcgaagat gctgattacc tttccggagc
    40681 caattgatga agcattattg acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt
    40741 cgcatccggc aaactggctg acgttacaac gtttaaacga tacccagtat gaagcccggg
    40801 ttccagtgag caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaatg
    40861 gtcagtacag ttttcagaac gccgggatca aagttgccgt tcctcagctt gatatccggg
    40921 tgaaaacgga caaaacccat taccagcctg gtgaactggt caatgtcgaa ttaacctcgt
    40981 cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat gaaatgatct
    41041 acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta
    41101 acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg
    41161 agccggttgc gcctggcgca actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc
    41221 cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag
    41281 gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc
    41341 gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa
    41401 atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa ccggcggcag
    41461 gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag
    41521 gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctggcgc
    41581 agaacattca gcaatctggc ttgttaagcg cagaactgca acaaaatggg caagtgcagg
    41641 acagcattag cacaaaactg tcttttgtgg ataacagctg gcccgttgaa cagcagaaaa
    41701 atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggct agcaatatcc
    41761 ggctacaaag tagcgaaacg ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg
    41821 atggaccatg gggtggcgtg atcaacaccg gtagccgtct gatcccgctc agtctcgcct
    41881 ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag atgattcagg
    41941 ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc tggtggggtg
    42001 aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac tggcaggcca
    42061 gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc gacagttatg
    42121 ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg tgggcacagg
    42181 agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc gcccggcgcg
    42241 gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac agcctgatcc
    42301 tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc atgacgttgc
    42361 tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat gcgtgggata
    42421 aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat agcggtggtg
    42481 acgctaccca gacggccgct attttaagtg gtttgaccgc tgagcaatcc actattgagc
    42541 gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca gttgttttgc
    42601 ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa gactggcgtt
    42661 gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg ccgcaaaatg
    42721 tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt ccggtgaccg
    42781 ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt actctgcagc
    42841 cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt acgcttacca
    42901 gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg ccgggagccg
    42961 acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg aaacagcagg
    43021 ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg gtgccggtga
    43081 aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa aaagggcaat
    43141 tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa agtgttgcgg
    43201 cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga atcgtctggc
    43261 tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa ctggtgctgc
    43321 gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc aaagtctcag
    43381 cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat gtttacgcct
    43441 ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac tccccggaag
    43501 aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg gtacgttcct
    43561 gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg gggcaatact
    43621 ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag ccagaaacct
    43681 cggtaacggt taaatcgctg ctcgattcat tagcgacgtt acctgcgcaa aacaaagctc
    43741 aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca tccatgctcg
    43801 gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag gaaatccgcc
    43861 agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact ggcagtggca
    43921 ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg ccagtgccga
    43981 cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga
    44041 aaatcacagc ggaaaagagt acgacggcag taaaacccgg cgtacttaat gggcgctatc
    44101 gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag acgaccttac
    44161 tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag tacgttgcca
    44221 gagttctgga tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg
    44281 cgattcgcac ttttttacag caaaacgcca atcgggaagg cgattgcctg accatccccg
    44341 acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg cgaacgatga
    44401 ccgcctggac gcaggatctc atctatgcag gcgatccggt tcattatcat ggcagtcgcg
    44461 ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag ggggagcgtt
    44521 acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg ggagctccac
    44581 gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa atgccgcagt
    44641 ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt gcggtctgtc
    44701 gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt attcgtaact
    44761 tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg gcctttgatg
    44821 gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa ttattaatgg
    44881 actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc gcctgtcgct
    44941 cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg gcatccggca
    45001 gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg
    45061 gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg
    45121 ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca gagagttgaa
    45181 tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag cgtgcaggtc
    45241 atcagcccgg atgggcaaag ccgacaaaaa atgcagtttt actcaacgcc gggtacagga
    45301 acgattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac cgatctcgat
    45361 ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt gctgaaaaac
    45421 agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt cgccatgcca
    45481 gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg cagcgaaacg
    45541 gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa tgagaaacag
    45601 gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa aagttttgac
    45661 tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga ctgctgggcg
    45721 atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca tttcttattt
    45781 ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg gtttctaagc
    45841 gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac cgctacctta
    45901 accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc ttgcttcttt
    45961 tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag tgctctggct
    46021 gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc gagaatcgga
    46081 aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc aacgcacctc
    46141 tgatgtgatg aaagagctga agtacatcgc cgaaaatcgc ttatcggcag aaaacggtgt
    46201 gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac cgctgtttgc
    46261 cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt cattggcgtg
    46321 gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc gggtattttt
    46381 aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc cagtggaacg
    46441 cgataccgcg ttgaaagctt tgcatgaacg catcaataaa tatcgaaatg caccacaaga
    46501 tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg tcgggtattt
    46561 ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg gtgtcgagca
    46621 gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg ttaccattct
    46681 tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa ttaagggcga
    46741 tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc gggagctggt
    46801 gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc cggttgataa
    46861 ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg tgcttgccgg
    46921 agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc cggcggaaag
    46981 cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg cctccgcgcc
    47041 agtgggtatc tgtattttgc gtaccgctga tggcgtcaat attttaagta acgaactggc
    47101 gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc aaattatctg
    47161 tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc tgcaaatcag
    47221 cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg tggatgtttc
    47281 ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg aacaggcgag
    47341 ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc cgctgtatgg
    47401 cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg tcgatcggct
    47461 ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg atattctcga
    47521 tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt caccgcgtga
    47581 agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc agttaggctt
    47641 gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga tgcgtttaca
    47701 gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg gctgtatagt
    47761 tttgcatgtc cgcgctgatg gcgattatct ctctatccgt gttcgcgata ccggcgtggg
    47821 gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg gaacgggcgt
    47881 acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac tgatcagcat
    47941 gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt ttaccgtgcg
    48001 tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt tgagtggtaa
    48061 acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa ccagtttgca
    48121 gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg aagatgtgtt
    48181 aattactgac gaggtagtga gtaaaaaatg gcagggtaga gcggtagtga ccttctgtcg
    48241 tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca gtgtggctgc
    48301 cccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg agagcgacga
    48361 tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataatgacg atatgatgat
    48421 tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt tgggatcgtt
    48481 gggctatcaa tgtaaaaccg cgaatgatgg tgtcgatgcg cttaatgtac ttagcaagaa
    48541 tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt accgcttgac
    48601 gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg ctaatgcgtt
    48661 ggctgaagtg aagcagcggt gtctggagtc cggtatggac agctgcctgt cgaagccggt
    48721 aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca ggaaatcgcg
    48781 ggaatcgtag gacggataag gtgtttatgc cgcatctggc attcagtgca aatgccagat
    48841 gcgacgctga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat aatgggaatc
    48901 gtaggccgga taaggcgttt acgctgcatc cggcaatcgg tgcaaatgcc agataagaca
    48961 ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact taaggtcaca
    49021 gaagagagat aattcagcag ggcgatatcg ttctcgacac ccagcttcat catcgcagat
    49081 ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat ctcggtcacc
    49141 aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag acgcttgtca
    49201 ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg ggtaaatttc
    49261 ttccctttct gcagcgcggc gagagctttc gacagatcgg tcggtgcacc ttgtttcagc
    49321 acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt gttgttcatg
    49381 gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat taaggtaatg
    49441 ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg cgcatccagt
    49501 ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac attcacccac
    49561 tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc atcggcaata
    49621 attacgttca tattgttcat gtaataggct accttgctac agcaagctct tgacataact
    49681 gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa tcagatgttc
    49741 cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata cgcctttaag
    49801 acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag tatacagcct
    49861 cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg catggagttg
    49921 cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca gttgcacttc
    49981 aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga cgcacaattg
    50041 accaggccca atttcccgta cgccagactc atcatcgctt aaaagcaagc cagaggcagt
    50101 aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta atctttcatc
    50161 gggtgtgata caggttgcac cccaattttc taactggcga gtgacaatat tccgaatttc
    50221 tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt cttcctcttc
    50281 aacttccggg tcagctgcga gcattttgat atgcacagag tagcgtgtac caagcccatc
    50341 ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc tcagccagaa
    50401 tgccagcggg tccgccttgc catagcgatc gttttgggtc tggttgataa acgggaagtg
    50461 caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc ggaacgtcag
    50521 gcggtcttcg gaggactcat cctgatcaac ctcaagggtg atttttccca attgcgttga
    50581 ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc gatcgccgcg
    50641 gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt tacgcttgat
    50701 ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga acagcaccgt
    50761 ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttcat caaccagccg
    50821 caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga gtttagccgc
    50881 gctctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt tcataaaggt
    50941 catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt tcttgtttac
    51001 cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg cgacctggct
    51061 gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa tcccctgatg
    51121 ctgttccgcc atggtggtga tgttttgcag attcaaatgc ggcagcaaat gatcggcaat
    51181 tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc cgagcggcag
    51241 cagtgagact atctcttcat taattgcccg taaaatgcgc aattcgttat tgaccgccgt
    51301 gctgggtaga ctttctgtac tgcggctgga gaaatggcgg aatgtggtat agccaaataa
    51361 cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt gcaacgtatc
    51421 cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag agttgagtgc
    51481 cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg tcccttcttt
    51541 ctcattatca ttgtctcccg ttgccgtcgc gtctggctca aggcggaaac tgtccagcgg
    51601 catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg ttgccagatg
    51661 tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc aggccaggcg
    51721 gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca acatttctgc
    51781 acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg tgagatcttt
    51841 gaggggcagg gttgagatca gcaccagact gttatcctga ccattcaggt aatacatcga
    51901 ccacggtaca ttttctgcgc cccacaatgt gtccagatag gtggacatcc gttgagtcat
    51961 ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc ggcgcggttt
    52021 ttccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc cggaggaggg
    52081 ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc aggtatcgac
    52141 gcgcttttgg atagcgtggg taatgtcgac aatctggtaa cttttatctt tcagccaggc
    52201 gttaacggcg ctttgtacca ttacccccat cgtcaccagt aacacaatga tcaacagtaa
    52261 aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg ttgtctcttt
    52321 ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc agtataaagg
    52381 gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc ccgtgatatt
    52441 tatcatcctg cattggggga aatagatgta caagcgccat tttgctgtac aaataaccta
    52501 caaaaagccc aacaaaaata attgcaaata aacaaagatt gctggaaatt atgctgatgt
    52561 taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca cagaataatg
    52621 aaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg aaatagggta
    52681 aacagacatt cagaaatgaa tgacggtaat aaataaagtt aatgatgata gcgggagtta
    52741 ttctagttgc gagtgaaggt tttgttttga cattcagtgc tgtcaaatac ttaagaataa
    52801 gttattgatt ttaaccttga attattattg cttgatgtta cgtgcttatt tcgccattcc
    52861 gcaataatct taaaaagttc tcttgcattt acattttgaa acatctatag cgacaaatga
    52921 aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa
    52981 tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata
    53041 aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag
    53101 ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat
    53161 tagatctgta cggtaaagta gacggcctgc actatttctc tgacaacaaa gatgtagatg
    53221 gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga
    53281 ccggttacgg ccagtgggaa tatcagattc agggcaacag cgctgaaaac gaaaacaact
    53341 cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg
    53401 gtcgtaacta cggcgttgtt tacgacgtaa cttcctggac cgacgtactg ccagaattcg
    53461 gtggtgacac ctacggttct gacaacttca tgcagcagcg tggtaacggc ttcgcgacct
    53521 accgtaacac tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg
    53581 gtaaaaacgg tagcgtaagc ggcgaaggca tgaccaacaa cggtcgtggt gctctgcgtc
    53641 agaacggcga cggcgttggc ggatctatca cttatgatta cgaaggcttc ggtatcggtg
    53701 gtgcgatctc cagctccaaa cgtactgatg atcagaacag cccgctgtac atcggtaacg
    53761 gcgaccgtgc tgaaacctac accggtggtc tgaaatacga cgctaacaac atctacctgg
    53821 ctgctcagta cacccagacc tataacgcaa ctcgcgtagg ttccctgggt tgggcgaaca
    53881 aagcacagaa ctttgaagct gttgctcagt accagttcga cttcggtctg cgtccgtctg
    53941 tagcatacct gcagtctaaa ggtaaaaacc tgggtaccat cgctggtcgt aactacgacg
    54001 acgaagatat cctgaaatat gttgatgttg gtgctaccta ctacttcaac aaaaacatgt
    54061 ccacctacgt tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg
    54121 gcatcaacac tgataacatc gtagctctgg gtctggttta ccagttctaa tctcgattga
    54181 tatcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa actcattttt
    54241 ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc
    54301 gcgtggcact gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg
    54361 ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt accttctggc
    54421 gtgccagcat cccgggcatt gatgccaaac gcagtgccga acttaaagaa aagattcaga
    54481 cccagctgga cgccgacgat cagctgcttt cgacctataa aaaagattcc acgctgatgc
    54541 gctttaacga ctcgcaaagt ttgtcgccgt ggccggtaag tgaagcgatg gccgatatcg
    54601 tcaccacctc gctgcgcatt ggcgcaagga ccgatggtgc gatggatata accgtcgggc
    54661 cgctggtgaa tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg
    54721 aacagatcga tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc atcaatcagt
    54781 cgcatcagca atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg
    54841 gtgaaggtta tgcggcggat cacctggcac gcttgatgga gcaggaaggt atttcacgtt
    54901 atctggtgtc agttggtggt gctctggata gccgtggtat gaacggtgaa ggccagccgt
    54961 ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggcc gtggtggata
    55021 tcaacggtca tggcattagc acctctggca gctaccgtaa ctattacgaa ctggacggca
    55081 aacgtctttc tcatgttatc gatccgcaaa ccgggcgtcc tatcgaacac aatctggtat
    55141 ccgtgacggt gattgccccg acggcgctgg aagccgatgc ctgggatact ggcttaatgg
    55201 tactcgggcc ggagaaagcc aaagaggttg ttcgccggga agggctggcg gtctatatga
    55261 tcaccaaaga aggtgatagc tttaaaacct ggatgtcgcc gcagtttaaa agcttcctta
    55321 tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtga ccgggcagac
    55381 taaaggctat ccttaaccag ggagctgatt atgaaaaaag ccacatgctt aactgacgat
    55441 caatgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc
    55501 gtgcgtacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa
    55561 aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ctggctttcg cccctgcaaa
    55621 cgttgtcagc cagaaaaagc caatgcccag caacatcggt tggataaaat cacccacgcg
    55681 tgtcgactgc tggaacagga aacgcctgta acgctggaag ccttagccga ccaggtggtg
    55741 atgagtccat ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc
    55801 tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg
    55861 acgacgtcta ttcttaacgc cggattcccc gacagcagca gt
//
LOCUS       DAHBHC010000024        55055 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHBHC010000024 DAHBHC010000000
VERSION     DAHBHC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.024"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..529
                     /gene="ada"
                     /locus_tag="M2A35_001837"
     CDS             <1..529
                     /gene="ada"
                     /locus_tag="M2A35_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCB7198148.1"
                     /translation="FRHGGENLAVRYALADCELGRSLVAESERGICAILLGDDDATLI
                     SELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRT
                     IPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGYRWGVS
                     RKAQLLRREAENEER"
     gene            529..1179
                     /gene="alkB"
                     /locus_tag="M2A35_001838"
     CDS             529..1179
                     /gene="alkB"
                     /locus_tag="M2A35_001838"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCB7198149.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            1255..2898
                     /gene="yojI"
                     /locus_tag="M2A35_001839"
     CDS             1255..2898
                     /gene="yojI"
                     /locus_tag="M2A35_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCB7198150.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            3116..4762
                     /gene="mqo"
                     /locus_tag="M2A35_001840"
     CDS             3116..4762
                     /gene="mqo"
                     /locus_tag="M2A35_001840"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCB7198151.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(4911..5399)
                     /gene="eco"
                     /locus_tag="M2A35_001841"
     CDS             complement(4911..5399)
                     /gene="eco"
                     /locus_tag="M2A35_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCB7198152.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(5534..5698)
                     /locus_tag="M2A35_001842"
     CDS             complement(5534..5698)
                     /locus_tag="M2A35_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198153.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            5807..6301
                     /gene="napF"
                     /locus_tag="M2A35_001843"
     CDS             5807..6301
                     /gene="napF"
                     /locus_tag="M2A35_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCB7198154.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDWQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            6291..6554
                     /gene="napD"
                     /locus_tag="M2A35_001844"
     CDS             6291..6554
                     /gene="napD"
                     /locus_tag="M2A35_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCB7198155.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            6551..9037
                     /gene="napA"
                     /locus_tag="M2A35_001845"
     CDS             6551..9037
                     /gene="napA"
                     /locus_tag="M2A35_001845"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCB7198156.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            9044..9739
                     /gene="napG"
                     /locus_tag="M2A35_001846"
     CDS             9044..9739
                     /gene="napG"
                     /locus_tag="M2A35_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCB7198157.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            9726..10589
                     /gene="napH"
                     /locus_tag="M2A35_001847"
     CDS             9726..10589
                     /gene="napH"
                     /locus_tag="M2A35_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCB7198158.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            10586..11035
                     /gene="napB"
                     /locus_tag="M2A35_001848"
     CDS             10586..11035
                     /gene="napB"
                     /locus_tag="M2A35_001848"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCB7198159.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            11045..11647
                     /gene="napC"
                     /locus_tag="M2A35_001849"
     CDS             11045..11647
                     /gene="napC"
                     /locus_tag="M2A35_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCB7198160.1"
                     /translation="MGNSDRKPGLIKRLWKWWRAPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            11666..12283
                     /gene="ccmA"
                     /locus_tag="M2A35_001850"
     CDS             11666..12283
                     /gene="ccmA"
                     /locus_tag="M2A35_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCB7198161.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            12280..12942
                     /gene="ccmB"
                     /locus_tag="M2A35_001851"
     CDS             12280..12942
                     /gene="ccmB"
                     /locus_tag="M2A35_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCB7198162.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            12984..13721
                     /gene="ccmC"
                     /locus_tag="M2A35_001852"
     CDS             12984..13721
                     /gene="ccmC"
                     /locus_tag="M2A35_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCB7198163.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            13718..13927
                     /gene="ccmD"
                     /locus_tag="M2A35_001853"
     CDS             13718..13927
                     /gene="ccmD"
                     /locus_tag="M2A35_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCB7198164.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            13924..14403
                     /gene="ccmE"
                     /locus_tag="M2A35_001854"
     CDS             13924..14403
                     /gene="ccmE"
                     /locus_tag="M2A35_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCB7198165.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            14400..16343
                     /gene="ccmF"
                     /locus_tag="M2A35_001855"
     CDS             14400..16343
                     /gene="ccmF"
                     /locus_tag="M2A35_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCB7198166.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            16340..16897
                     /gene="dsbE"
                     /locus_tag="M2A35_001856"
     CDS             16340..16897
                     /gene="dsbE"
                     /locus_tag="M2A35_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCB7198167.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQVDVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            16894..17946
                     /gene="ccmH"
                     /locus_tag="M2A35_001857"
     CDS             16894..17946
                     /gene="ccmH"
                     /locus_tag="M2A35_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCB7198168.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(17981..18628)
                     /gene="narP"
                     /locus_tag="M2A35_001858"
     CDS             complement(17981..18628)
                     /gene="narP"
                     /locus_tag="M2A35_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCB7198169.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            18948..21539
                     /gene="yejO"
                     /locus_tag="M2A35_001859"
                     /pseudo
     CDS             18948..21539
                     /gene="yejO"
                     /locus_tag="M2A35_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(21643..21719)
                     /locus_tag="M2A35_001860"
     tRNA            complement(21643..21719)
                     /locus_tag="M2A35_001860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21683..21685),aa:Pro,seq:ggg)
     gene            complement(21794..23554)
                     /gene="pbgA"
                     /locus_tag="M2A35_001861"
     CDS             complement(21794..23554)
                     /gene="pbgA"
                     /locus_tag="M2A35_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCB7198170.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(23574..23801)
                     /locus_tag="M2A35_001862"
     CDS             complement(23574..23801)
                     /locus_tag="M2A35_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCB7198171.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            23983..24990
                     /gene="yejK"
                     /locus_tag="M2A35_001863"
     CDS             23983..24990
                     /gene="yejK"
                     /locus_tag="M2A35_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCB7198172.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(25129..25413)
                     /gene="rplY"
                     /locus_tag="M2A35_001864"
     CDS             complement(25129..25413)
                     /gene="rplY"
                     /locus_tag="M2A35_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCB7198173.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(25538..27298)
                     /locus_tag="M2A35_001865"
     CDS             complement(25538..27298)
                     /locus_tag="M2A35_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCB7198174.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            27447..28142
                     /gene="rsuA"
                     /locus_tag="M2A35_001866"
     CDS             27447..28142
                     /gene="rsuA"
                     /locus_tag="M2A35_001866"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCB7198175.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            28170..29360
                     /gene="bcr"
                     /locus_tag="M2A35_001867"
     CDS             28170..29360
                     /gene="bcr"
                     /locus_tag="M2A35_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCB7198176.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            29693..30037
                     /locus_tag="M2A35_001868"
     CDS             29693..30037
                     /locus_tag="M2A35_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198177.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(30041..31630)
                     /gene="yejF"
                     /locus_tag="M2A35_001869"
     CDS             complement(30041..31630)
                     /gene="yejF"
                     /locus_tag="M2A35_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCB7198178.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSAQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(31632..32657)
                     /locus_tag="M2A35_001870"
     CDS             complement(31632..32657)
                     /locus_tag="M2A35_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7198179.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(32657..33751)
                     /locus_tag="M2A35_001871"
     CDS             complement(32657..33751)
                     /locus_tag="M2A35_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCB7198180.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMIWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(33752..35566)
                     /locus_tag="M2A35_001872"
     CDS             complement(33752..35566)
                     /locus_tag="M2A35_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCB7198181.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(35648..37204)
                     /locus_tag="M2A35_001873"
     CDS             complement(35648..37204)
                     /locus_tag="M2A35_001873"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCB7198182.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(37385..37951)
                     /gene="mepS"
                     /locus_tag="M2A35_001874"
     CDS             complement(37385..37951)
                     /gene="mepS"
                     /locus_tag="M2A35_001874"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCB7198183.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(38363..39076)
                     /gene="lpxT"
                     /locus_tag="M2A35_001875"
     CDS             complement(38363..39076)
                     /gene="lpxT"
                     /locus_tag="M2A35_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCB7198184.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VLLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(39115..40101)
                     /gene="yeiR"
                     /locus_tag="M2A35_001876"
     CDS             complement(39115..40101)
                     /gene="yeiR"
                     /locus_tag="M2A35_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCB7198185.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(40219..41685)
                     /locus_tag="M2A35_001877"
     CDS             complement(40219..41685)
                     /locus_tag="M2A35_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCB7198186.1"
                     /translation="MKTIASVTLPHHIHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(41908..42480)
                     /gene="yeiP"
                     /locus_tag="M2A35_001878"
     CDS             complement(41908..42480)
                     /gene="yeiP"
                     /locus_tag="M2A35_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCB7198187.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42635..42889
                     /locus_tag="M2A35_001879"
     CDS             42635..42889
                     /locus_tag="M2A35_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCB7198188.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(42886..44067)
                     /gene="setB"
                     /locus_tag="M2A35_001880"
     CDS             complement(42886..44067)
                     /gene="setB"
                     /locus_tag="M2A35_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCB7198189.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            44435..45565
                     /gene="fruB"
                     /locus_tag="M2A35_001881"
     CDS             44435..45565
                     /gene="fruB"
                     /locus_tag="M2A35_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCB7198190.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSAPTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            45565..46503
                     /gene="fruK"
                     /locus_tag="M2A35_001882"
     CDS             45565..46503
                     /gene="fruK"
                     /locus_tag="M2A35_001882"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCB7198191.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            46520..48211
                     /gene="fruA"
                     /locus_tag="M2A35_001883"
     CDS             46520..48211
                     /gene="fruA"
                     /locus_tag="M2A35_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCB7198192.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            48634..49575
                     /locus_tag="M2A35_001884"
     CDS             48634..49575
                     /locus_tag="M2A35_001884"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCB7198193.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            49563..50501
                     /gene="psuG"
                     /locus_tag="M2A35_001885"
     CDS             49563..50501
                     /gene="psuG"
                     /locus_tag="M2A35_001885"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCB7198194.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            50595..51845
                     /locus_tag="M2A35_001886"
     CDS             50595..51845
                     /locus_tag="M2A35_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCB7198195.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(51916..52614)
                     /gene="yeiL"
                     /locus_tag="M2A35_001887"
     CDS             complement(51916..52614)
                     /gene="yeiL"
                     /locus_tag="M2A35_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCB7198196.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            52744..53685
                     /gene="rihB"
                     /locus_tag="M2A35_001888"
     CDS             52744..53685
                     /gene="rihB"
                     /locus_tag="M2A35_001888"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCB7198197.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            53785..55035
                     /locus_tag="M2A35_001889"
     CDS             53785..55035
                     /locus_tag="M2A35_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCB7198198.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT        13140 a        13470 c        14956 g        13489 t
ORIGIN      
        1 attccgtcac ggtggcgaaa atctggcggt gcgttacgcg ctggctgatt gtgagctggg
       61 tcgtagcctg gtggcagaaa gcgagcgggg gatttgcgcg atattgctgg gcgatgatga
      121 cgcgacacta atcagcgagt tgcagcagat gtttcccgct gccgacaacg cgcctgccga
      181 tctgatgttt cagcaacatg tgcgtgaagt gatcgccagc ctcaatcaac gcgatacgcc
      241 gctgacgtta ccgctggaca ttcgcggcac tgcttttcag caacaagtct ggcaggcact
      301 gcgcacgata ccttgcggtg aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa
      361 accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg ctatcataat
      421 accctgtcat cgggtggtcc gtggtgatgg cacactttcc ggttaccgct ggggcgtgtc
      481 gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat gttggatctg
      541 tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat tttacggcgt
      601 tttgctttta atgctgcgga gcaactaatc cgcgatatta atgacgttgc cagccagtcg
      661 ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat gaccaactgt
      721 gggcatctgg gctggacgag ccatcggcaa ggttatctct attcgcccat tgatccgcaa
      781 acaaataaac cgtggcccgc catgccacag agttttcata atttatgtca acgtgcggct
      841 acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg ctacgctcct
      901 ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc gccaattgtt
      961 tctgtttctc tgggcttacc tgcgattttt caatttggtg gcctgaaacg taatgatccg
     1021 ctcaaacgtt tgttgttgga acatggcgat gtggtggtgt ggggcggtga gtcgcggctg
     1081 ttttatcacg gtattcaacc gttgaaagcg gggtttcatc cactcaccac cgactgccgc
     1141 tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt attattgctg
     1201 tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt ctgcatggaa
     1261 cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat ggcgctaagc
     1321 ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg ccttatcgaa
     1381 acggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct gctgttgatg
     1441 gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt cgtttaccga
     1501 ctgcgtagtg aatttatcaa gcggattctg gataccaacg tcgagcgcat tgaacaactc
     1561 ggtagcgcct cgttgctggc gggattaacc agcgatgtgc gcaatatcac cattgctttt
     1621 gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc ggcgtatctg
     1681 tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat caccatctgg
     1741 ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctgcg tgaaaccgaa
     1801 gacaggctgt atacggattt tcaaactgta ctcgaagggc gcaaagagct gactctgaac
     1861 cgggaacgcg ccgagtatgt gtttaacaac ctctacattc ctgatgcgca agagtatcgc
     1921 caccatatta tccgcgcaga caccttccat cttagcgccg tgaactggtc aaacatcatg
     1981 atgttgggcg caatcggcct ggtgttctgg atggcgaaca gtctcggttg ggctgatacc
     2041 aacgttgccg cgacctattc gttgacgctt ttattcctgc gtacgccgct gctttcggcg
     2101 gttggcgcat tgccgacgct gctgacggcg caggtggcgt ttaacaagct gaacaaattc
     2161 gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa ctggcaaacg
     2221 ctggagctgc gtaacgtgac gtttgcttat caggataacg cgttttccgt tggtccgatt
     2281 aatctcacca tcaaacgtgg cgagctgctg tttctgattg gcggcaacgg tagcggaaaa
     2341 tcgacgctgg cgatgttgtt gacgggcttg tatcaaccac aaagcggcga aatcttgctg
     2401 gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg
     2461 tttaccgatg tctggctgtt tgatcaactg ctggggccgg agggtaaacc cgctaacccg
     2521 caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga gttaagcaac
     2581 gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc gctgttgctg
     2641 gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga tcaggatcca
     2701 cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat gggtaaaact
     2761 attttcgcca tcagtcatga tgatcattac tttatccacg ccgaccgcct gctggaaatg
     2821 cgcaacgggc aacttagcga gctgacgggc gaagagcgcg atgctgcttc gcgtgatgcc
     2881 gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg ccaggtcaat
     2941 atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat tataatgttt
     3001 ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag tgttaccgtt
     3061 gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta acaagatgaa
     3121 aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt cgatggcggc
     3181 aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg gcggcattat
     3241 gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga tgaccatggt
     3301 ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg ccggaaccgg
     3361 gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca gcatcagtat
     3421 tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct gggcgcacca
     3481 ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc cgcatatgag
     3541 ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg cgttgcaaca
     3601 aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca aagagtgggc
     3661 acctttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc gtacggaaat
     3721 tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct tgcagaagaa
     3781 atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta atgacgataa
     3841 cacctggacc gttaccgttg ccgatctaaa aaatggcact gcacagaaca ttcgtgccaa
     3901 atttgtcttt atcggcgcgg gcggtgcggc gctgaagcta ttacaggaat cggggattcc
     3961 ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt cggaaaaccc
     4021 ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg gcgcaccacc
     4081 gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag tgctgtttgg
     4141 gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg atctgatgag
     4201 ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata atttcgatct
     4261 ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg aagcgttgaa
     4321 agagtactat ccgcaagcga aaaaagagga ctggcgtttg tggcaagcgg ggcagcgcgt
     4381 gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta ctgaagtggt
     4441 cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg cgtcaaccgc
     4501 cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt ccagcccgca
     4561 atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga acggtgatgt
     4621 agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga aatacgacaa
     4681 gccgcaggca gcagatagta cgccaaaacc gcagttgaaa ccgcaacccg ttcaaaaaga
     4741 agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc ggatgtggcg
     4801 tatgctgata agacgcgcca gcgtcgcatc aggcaccggc tcgggcgtta gatgtcggat
     4861 gcggcgtgac cgccttattc ggcctacggg gtgccgcact tcacagcatt ttagcgaact
     4921 accgcgttgt caattttctc ttccgccttc cagacgcggt acttcacatc tacattgtct
     4981 ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca gcattccagc atcgcccaga
     5041 tacgcggtga caaatttctt ctctttcttg ccatccgggc aggccatcat cgtggaaacc
     5101 ggcgagctaa ctttatcaaa gacataataa tcatagcccc agccttccag cgttttgttt
     5161 tccagcttcc cgccgagacg atgcaaattg caatcgactt ccagcgtctg accgattaac
     5221 agttctactt tcagggtaga ttcatcttct tgcggggtta actgaatcac ctgacgcttc
     5281 atcccttttt cagcttgtgg ataaggcgcg attttttcca gtggctggac gctttctgcc
     5341 gcccaggcgg aagtggtagc gaaagcggca aacaatactg caggtagaat ggtcttcatt
     5401 tttttgcttt ccttcttcat ttataggttt tcaccgatga tgtcgctgaa ggttaacata
     5461 ttcatcagat ttattaattt gctttatgaa taacttaaag aacattcaga caattttacc
     5521 tgctctttta gtgttaaatt ctaatgagag agcaaaccag cagaaatatc tctggtgaat
     5581 taatcgctat ataaatatat ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc
     5641 acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg gtatgcatct
     5701 ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta tgcggtttat
     5761 ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga agattgatgc
     5821 atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc
     5881 ctggtccggt gatgaatctc attttctgac ccattgcacc cgctgcgacg cctgtattaa
     5941 tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa
     6001 aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat cgctattttc
     6061 tccgcgccac accagggcct gggattggca gttcaccatt ggggacgcgt gcctggcgta
     6121 tcagtctgtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa ttatctttcg
     6181 cccgacgctc tccgggatct accagccgca acttaatagc caactttgca acggatgtgg
     6241 agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc atgcacacta
     6301 actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca gacatcagca
     6361 cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt
     6421 tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt gagtcagtac
     6481 gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg
     6541 aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc
     6601 tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga
     6661 agccatcaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg gcgttctggt
     6721 cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac cggttaaccg
     6781 tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta aagaccgttt
     6841 gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg aatttacccc
     6901 aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga
     6961 aaaaggaccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta
     7021 tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac aacatcgacc cgaacgcacg
     7081 tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg atgagccgat
     7141 gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg ctaacatggc
     7201 ggagatgcac ccgatcctct ggtcacgcat cactaaccgt cgtctctcta accagaacgt
     7261 caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat
     7321 catctttacg ccgcaatctg acctggtgat cctgaactac atcgccaact atatcattca
     7381 aaacaatgcg ataaatcagg acttcttcag caagcacgtt aacctgcgca aaggggcgac
     7441 ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccagg
     7501 ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg ccgagtatac
     7561 gctggaaaaa actgccgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca
     7621 gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca
     7681 tactcgtggc gtgtgggcca acaacctggt ctacaacctg cacctgctga ccggcaaaat
     7741 ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttccgcgt gtggtactgc
     7801 gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga ctaacgagaa
     7861 acaccgtgat atttgcgaga agaagtggaa tatcccgagc ggcaccattc cggcgaaaat
     7921 cggtctgcac gcggtggcac aagaccgtgc actgaaagac ggcaagctga atgtttactg
     7981 gaccatgtgt accaacaaca tgcaggccgg gccgaacatt aatgaagagc gtatgccggg
     8041 ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag tcagtgcgct
     8101 ggcagccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggcg cttacggtaa
     8161 cgccgaacgc cgtactcagt tctggcgtca gcaggtacag gcgccaggcg aagcgaaatc
     8221 ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag tatggccgga
     8281 agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag ttctgtatgc
     8341 cacccccgaa gtgagcaaat tcccggtatc cgaactggcg gaagatcagc tgaacgatga
     8401 atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg catggtttgg
     8461 tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg
     8521 ctggccggtg gtgaacggta aagaaacaca gtggcgttac agcgaaggta acgacccgta
     8581 cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg gatggcaaag cggtgatctt
     8641 cgcactgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc tgtggctctc
     8701 taccggacgc gttctggagc actggcacac cggcagtatg actcgccgtg tgccggaact
     8761 gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg ctggatgcga aagcgcgcga
     8821 tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggtgaagtga tctcgattgt
     8881 tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt tcttcgacgc
     8941 cgcacagctg gttaacaaac tgacgctgga tgcgaccgat ccgctctcga aagagacgga
     9001 cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa
     9061 acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt cgcacagcag gcgggctggc
     9121 tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt
     9181 gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca
     9241 gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggcct ctggtctgtc
     9301 ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg aggacattcc
     9361 gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag attgaatcga tcgacgacgc
     9421 gcggatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc aggggctgcg
     9481 ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc tggagctgga
     9541 gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca gcgacgcctg
     9601 taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa caaccggcaa tcaaggtgtt
     9661 accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggtt ggctggaggg
     9721 gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa aaaggctggt
     9781 ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt
     9841 ttttgagcgg tccatggttt ggtgtgtgga tcttgcacgg caactacagc agtagcctgt
     9901 tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt gccagtggtc
     9961 atctgcctgc cacggtggcg ttgaccggtg cggtgattat caccgtgctc tacgccctgg
    10021 cgggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc gacttagcga
    10081 actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc cacattcgct
    10141 acgtgctgtt ggtggtcatt ctgctgggat cggccttaac tggcacgctc atctgggaat
    10201 ggatcaaccc cgtatctctg atggggcgca gcctggtaat gggcttcggc agcggcgcgc
    10261 tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc tggtgcgggc
    10321 acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg attaccgttg
    10381 cggccaccga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt tgcccggaac
    10441 cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc accagccgcg
    10501 attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt acaataacta
    10561 cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca
    10621 atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta atggcgtcga
    10681 ttttagccag tcgccggaag tatccgggac acaggaaggg gccattcgga tgccaaaaga
    10741 gcaggaacgg atgccgctga actatgtgaa tcagccgcca atgatcccgc atagcgttga
    10801 aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg tcgaaagtta
    10861 tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg acggcaaagt
    10921 gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga
    10981 taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga aataagaggt
    11041 tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga aatggtggcg
    11101 tgcccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgttg gcggcatcgt
    11161 cttctggggt ggctttaaca ccgggatgga aaaagccaat accgaagagt tctgcattag
    11221 ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg gattccgtgc actacaacaa
    11281 ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa
    11341 gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg gcgttattga
    11401 cacgccgcag aaatttgaag ctcatcgtct gacgatggca cagaatgagt ggcggcgcat
    11461 gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata tggatacaac
    11521 cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg
    11581 tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg
    11641 tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt gtgagcggga
    11701 tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt gggtacaaat
    11761 caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg
    11821 ccctgacgca ggcgaggttc tctggcaagg gcagcccttg catcaggtac gcgacagcta
    11881 ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc tgacggcgtt
    11941 agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg aagccctggc
    12001 gcaggccggg cttgccggat tcgaagatat tcctgtaaat cagctctcgg ccgggcaaca
    12061 acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga tcctcgacga
    12121 gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta tggcgcagca
    12181 tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg ttgctgaaag
    12241 taaaattcgc cgcatttcac tgacgcaaac gagggccgca tgatgttctg gcgcattttc
    12301 cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc
    12361 ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca actgctggcg
    12421 cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct ggcgctggaa
    12481 cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt gttgccgtta
    12541 cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac cggtctgccg
    12601 ttactcattc tttcgccact ggtagcaatg ctactgggaa tggatgttta tggctggcaa
    12661 gtgatggcgc tgacgctgct gctgggaacg cctacgcttg gctttctcgg tgcaccgggc
    12721 gtggcgctga cagtgggact taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg
    12781 ctgactatcc cattactcat cttcgccacc gccgcgatgg acgcggcttc catgcatttg
    12841 cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc gacattaagt
    12901 ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat tactgatttt
    12961 tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg atcccaccac
    13021 ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt gtggtcgtgc
    13081 ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag cagggaaata
    13141 gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc atttatgcat
    13201 caatggcagt ggcagcgttt attggccttg tctggcagat gaaaatggcc aacctggcgg
    13261 tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt accggctctg
    13321 catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg acttctgaac
    13381 tggtgctgct ttttttgtat gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc
    13441 gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta
    13501 ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa
    13561 gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt
    13621 ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa aaacgccgtc
    13681 cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg
    13741 gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg tggtgatgac
    13801 cgttattccg ctggtggttt tggtcgtgca ctcggtgatg caacatcgcg caattctgcg
    13861 tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc
    13921 tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg
    13981 gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct cttttatacg
    14041 ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt
    14101 ctgcgcgttg gcgggatggt gatgccgggt agtgtgcagc gcgatcccaa ttcgctgaaa
    14161 gtgaccttca ctatttacga tgctgaaggc tcagtggatg tctcttacga aggcattttg
    14221 ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat
    14281 catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc gccagaagtt
    14341 gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga cccagcatca
    14401 tgatgccgga aattggaaac gggctgctgt gcctggcgtt agggattgcg ctgctgctgt
    14461 ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg catgatggcg tcttcccgct
    14521 tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt
    14581 tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa ctccaatacc cagcttccgg
    14641 tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctcctg ctgtgggtgc
    14701 tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt ccgctggata
    14761 ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg ctgttcattc
    14821 tctttacctc taacccattc tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc
    14881 ttaacccgct gttacaggat ccggggctga tcttccatcc gcctctgctt tatatggggt
    14941 acgtgggttt ctcggtggcg tttgcttttg ccattgcttc tttgttgtgc gggcgtctgg
    15001 acagcactta tgcgcgtttt actcgtccgt ggacgctggc ggcgtggatc ttcttgacgc
    15061 tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc ggctggtggt
    15121 tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact gcgctgatgc
    15181 actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta ctgctggcaa
    15241 tcagtgcctt ctcgttgtgt ctgctgggga ctttcctggt gcgttccggc gtgctggtat
    15301 cggtacacgc gtttgcctct gatccggcac gcggtatgtt tatcctcgcc tttatggtac
    15361 tggtgattgg tggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg
    15421 taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg
    15481 ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag caactgggac
    15541 tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg ctgatggtgc
    15601 cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta
    15661 aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg ctgctgctgc
    15721 cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggcctggca atggcctgct
    15781 ggattgctgt gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc acgaaaacca
    15841 ccttcagtta ttggggaatg gtggcggctc acctggggct ggcagtgaca attgttggta
    15901 ttgcctttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc ggcgatagcg
    15961 tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact
    16021 ggcgtggcgg tgtggcgact atcggcgtaa cgcgcgatgg caagccggaa acggtgctgt
    16081 atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg gcaattgacg
    16141 gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg
    16201 ccgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg ctgatgatgg
    16261 cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa
    16321 aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta attccgttga ttatcttcct
    16381 ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg atgatccgac
    16441 caatctggaa tcggcgctca ttggcaagcc tgtgccgaag tttcgtctcg aatcactgga
    16501 caatccgggg cagttttatc aggtggatgt gctgactcag ggcaaaccag tgctgcttaa
    16561 cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga atcagctttc
    16621 agcgcagggc attcgcgtgg tcggcatgaa ctataaagac gatcgccaga aggcaatcag
    16681 ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg acggcatgtt
    16741 agggctggat ctcggtgtct atggcgcgcc agaaaccttc cttattgacg gcaacggtat
    16801 cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc
    16861 gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg cgtgctgatg
    16921 ctgatgatct ccggctcagc actggcgacc atcgacgtgt tgcagtttaa agatgaagca
    16981 caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg ccagaacaac
    17041 agcattgccg attccaactc gatgattgcc accgacctgc gtcagaaagt gtatgaactg
    17101 atgcaggaag gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg ttacggcaac
    17161 ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt gcttccggta
    17221 gtggctattg gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgta
    17281 gtgccggaag cgtttcctga acaaagcgtg caggaaggta agcgtgccgg atatattgtt
    17341 tatctgccgg gtattgtggt ggcgttaatt gtggctggcg tcagctacta ccagactggc
    17401 aattatcagc aggtgaaaat ctggcagcag gccacggcac aggctccggc gttgctggac
    17461 agggcgctgg atccgaaagc cgatccgctc aacgaagaag agatgtcgcg tcttgcgctg
    17521 gggatgcgta ctcaactgca aaaaaatccg ggagatatag aaggctggat tatgttgggc
    17581 cgcgttggca tggcgctggg taacgctagt atcgccaccg atgcatacgc tactgcgtat
    17641 cgcctcgatc cgaaaaacag cgatgccgca ctgggttatg ctgaagcgtt gacacgttca
    17701 tctgatccca acgacaaccg cctcggtggt gaactgctgc gccagttggt gagaagcgac
    17761 cacagcaata tccgtgtgtt aagcatgtat gcgtttaatg cctttgagca gcagcgattt
    17821 ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa cgatactcgc
    17881 cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt
    17941 aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt
    18001 gcaggaacag aatggtggct gccacgcgtg agcggacatt gagtttgcgc agcagattgc
    18061 gaatatgtac ttttactgtc tgctctgaaa tattcaacac cgaggcaatc tgtttatttg
    18121 acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg
    18181 gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg cgttcgctaa
    18241 agactttgct gcctttcgct ccagcacgaa ttgcttccag caatacttcc gggtcgctgt
    18301 ctttcaacag ataaccgtct gcgcctgcgt ctatcagtgc aaagacatcg ctggaggcat
    18361 ctgatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag gcattgaggg
    18421 tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg atatccagtc
    18481 tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag
    18541 gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca tccacaatca
    18601 tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt agaatcatta
    18661 tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca ttttatgtat
    18721 ggacatgtag tgaaaactac aaccttgtcg ttcgctttgg catcattttc attataaaga
    18781 cggaattttg catcagcttc tcatcaagaa aaacaactaa aaaatcaatc acaatacttg
    18841 tgcatttctt taaacagttt ttaaaaactg actccgggta tggagctatg ggtattttcc
    18901 gtacccaatg cttttaacag caattaattt cataggatga aagctcaatt catcaatctg
    18961 gttctgtttc tctttgtcgt tccgcaatat ctgttctgat ggctacagcg ttatattcac
    19021 ccatagcatt ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt gtcctggaaa
    19081 aagatatcca gctggtttat gggaccgcca ataatacgaa aatcaatcct ggcggagaac
    19141 agcatataaa agaatttggt gtaagtagta atactgaaat taaaggaggg tatcagtaca
    19201 ttgaaatgaa tggtaccgca gaatactcag tattaaatga cggttatcaa attgttcaaa
    19261 tgggtggcgc ggcaaaccag actaccctca ataatggtgt gttacaggtt tatggcgcag
    19321 cgaatgatcc cacgattaaa ggcgggcgct taatcgttga aaaagatggg attaccgtcc
    19381 ttgccgctat cgaaaaggga ggattactgg aggttaaaga ggggggatta gcgattgcgg
    19441 tagatcagaa agcaggcggt gctattaaag caagcacgcg ggccatggag gtattcggaa
    19501 caaaccgtct cggtcagttc gatatcaaga atggtattgc taataatatg ttgttggaaa
    19561 acggcggaag tttgcgagtt gaagaaaatg acttcgctta taataccact gtagatagtg
    19621 gcggcttact ggaggttatg gatggcggga ctgtaactgg cgttgataaa aaagcaggcg
    19681 gaaaattaat tgtctcaacg aatgcgctgg aagtgagtgg tccaaacagt aaaggccaat
    19741 ttagtataaa agatggtgtg tcaaaaaatt atgaactgga tgatggttcc gggctcattg
    19801 ttatggagga cacgcaggcc attgatacta tccttgatga gcatgccact atgcaatcgc
    19861 tgggaaagga tactggtacg aaagtgcagg caaatgcgat atatgatctc ggtcgatcag
    19921 atcagaatgg aagtatcacg tattcctcaa aagccatctc tgaaaatatg gttatcaaca
    19981 atggccgcgc taacgtctgg gctggcacaa tggttaacgt ttcagtcaga gggaatgatg
    20041 gcattcttga ggtcatgaag ccgcaaataa attatgcacc cgcaatgttg gtgggtaagg
    20101 tagtggtttc tgagggcgct tcttttagaa cgcatggtgc cgtggatacc agcaaagcgg
    20161 acgtttcgct cgaaaatagc gtatggacca tcattgccga tatcactacg acgaaccaaa
    20221 acaccctcct caacttagcc aaccttgcga tgtctgacgc aaatgtgatt atgatggatg
    20281 agccagtgac tcgttcatca gtgacggcaa gtgcggaaaa tttcattacg ttgaccacca
    20341 ataccctgtc gggaaacggc aatttttata tgcgtaccga tatggctaat catcagagcg
    20401 atcagctcaa cgtcaccggt caggcaacag gtgatttcaa aatattcgtg acggacaccg
    20461 gtgccagccc ggcagcagga gatagcctta cactggtaac aacgggcggc ggtgatgctg
    20521 catttacgtt gggcaatgcc ggaggcgttg ttgatatcgg tacgtatgaa tataccttgc
    20581 tggataatgg caaccatagc tggagtttgg cagagaatcg cgcgcaaatt accccttcaa
    20641 ccactgatgt gctgaatatg gcggcagcac aaccgctggt atttgatgca gaactggaca
    20701 ccgtgcgtga gcgtcttggt agcgtaaaag gcgttaatta cgatacggcg atgtggagtt
    20761 cggcaattaa cacccgcaac aacgtgacca ctgatgcggg cgctggcttt gagcaaacat
    20821 tgaccgggct gacgctcggt atcgatagcc gtttctcccg tgaagaaacc agcacaattc
    20881 gcggcttgtt ctttggttac tctcattctg atattggttt tgatcgcggc ggcaaaggca
    20941 atgtcgatag ctataccctg ggggcttatg ccggttggga gcatcagaac ggtgcctatg
    21001 ttgatggagt ggtgaaagtt gaccgttttg ccaacaccat ccattgcaag atgagtaatg
    21061 gggcaacagc gtttggcgat tacaatagta acggcgcggg tgctcatgtc gagagcgggt
    21121 tccgttgggt tgacggattg tggagtgtta gaccctatct ggcctttacc ggctttacca
    21181 cagatggtca ggactacacg ttatcaaacg gcatgcgcgc ggatgtggga aatacccgga
    21241 tattacgcgc tgaagcggga acggcggtaa gctatcacat ggacctgcaa aacggtacga
    21301 cgctggaacc ctgactgaaa gccgccgtgc gtcaggaata cgccgattct aaccaggtga
    21361 aagttaatga cgatggcaaa tttaataatg atgtggctgg aacccgtggc gtttatcagg
    21421 ctgggataag gtcatcgttt accccgacgt taagcggtca tttgtcagtc agctatggca
    21481 atggcgcagg ggtagaatcg ccgtggaata ctcaggcggg tgtggtctgg acgttctgat
    21541 aacaaaaaat aaacaggctg tgatgtgtca cggtctgttt atcgaattaa ttgcagatat
    21601 aaaaaaaacc aaccgtaaag gttggttttt tcttgggatt tttggtcggc acgagaggat
    21661 ttgaacctcc gacccccgac accccatgac ggtgcgctac caggctgcgc tacgtgccga
    21721 ctcgtggctg ctaatactac cgttttccac accgattgca agtaagatat tttgctaact
    21781 gatttataat taatcagtta gcgataaaac gcttctcgtc tgtcagcact tgcaataaca
    21841 aacttaactg tggtttttca tctttcactc tttcaccacg taagttgtaa gtgcggtatt
    21901 taccgttatt gttcagcacc agcgtctttt tcggggtggt aattgccagc gtatcgttat
    21961 ccgctgcagt aacccagtaa tgacggcgtt gagggttgaa caaatcctga ccttgcgaat
    22021 attcgctggc aggtgtgctg acatgtagca ggcgttgcat cagcgtcgtc atcagatcgg
    22081 tatgatcagt cagagcatta atacgctgcg ccggcgtgcc tggccagtga atcactaatg
    22141 gcacctgcag atgaccgtgg gaccagtcaa aggtttcttc gtcttcgctc agcggaatac
    22201 cccgaccggc agtgataatc accaccgtat tgtccagttt gccagaatca cgcagtgcat
    22261 tgagcacgcg gttgatctgg tcatcgacat tgcctgccgc ccggctatat ttccgtgcaa
    22321 atgcctgctg attgctgtcg tcaatgttag tgccattgaa agagacccac gagaaccagc
    22381 ggttatcttc ttgtgcgtag cggcccagcc agttgatcca ctgcgtggcg gtctgctcgt
    22441 cggattgggt gcgtacgctc ggcatcgaga aatctgacaa caatgcctgg cgatacagcg
    22501 ggctggtaaa gccatctgat gagaataacc ccagctgata gccttgctga ttaagcgcag
    22561 taattaatgc cgcaggcgta cgggtcgaca gaatgccgtc catatagctc ggcgagatgc
    22621 catagaacag gccaaagatg ccgttgtctg tagtgttgcc ggagctcata tggcgcgtga
    22681 acgaaatatt ttgctcagca aaacctgcca gcgcaggcat ctgcttctcg aagcgtgagt
    22741 agttcaggcc atcgacagta atcaacagca cattctgccc ggtgcccata tcgcgatagc
    22801 gcagttcgct taacggatac tgaacggaaa cggcgtctgg attaccttgc tcaataagac
    22861 ggcgttgata ctcctgcgca tcaagcagac catgcttctc aagaaaacgt cgcgccgtca
    22921 tcgggtacga aagcggcagg ttagcgcgct gcatggtgat cgggcgatag aagttggcat
    22981 cggcccagat atacaccaca tgcgaggcga taaaggcgat aaataagaat gcggccagcg
    23041 ggcgcgcgaa gcgtcgacga cgcgtcaggc tgcgcagctt ttgccagctc cacgtcgcaa
    23101 acaccagttc aagcaataaa ataaccggca cgctgatgaa catcagctgc cagtcgcgcg
    23161 ccatctcatt ttcgtctggg ttgataacca gttgccagac gatgggatta agatggagat
    23221 ggaaacgagt aaagacttcg ctatcgatca gtaatagcgt cattcccgcc gttgccagaa
    23281 tgacggacaa aaacctcatc agcctctggg agccgacgat aaaggtcagc gggaagagga
    23341 tcagcaagta ggtggcgaac accaggaagc tgaaatggcc gataatgctt acgtaggaat
    23401 aaatgcgacc agcaagcgtt gtcggccagt cggcgataaa caggtaacgg ctgccaatga
    23461 cgagcgaaag cagaatattg aacagtgcaa accagtgccc ccaactgacc atctgggaga
    23521 ctttttcacg gtagcgctga cgatgagtta ccataacctg ttatctgttt cccttagtgc
    23581 gctttgtctt cgttgataga ggactgtaag gcgcgggcaa aagagttggc aattgcctgg
    23641 cgttgggccg gggcaatgct ggtgttgata aggttagtga ccatgttgcc taacaccatc
    23701 agggaaaggt cggtcggagc cttatgtttt tccagtacgt tgagcagctc tgcgagcaat
    23761 tgttcaacct gttcatcact gtagcgggaa atttgtggca taaatcgaaa tcagttagtt
    23821 gatgaaaggg caacatatta ccgtagcaac ggtttttttt ctgctttttt atgcgttaaa
    23881 tcattctcgt tacgctgcct tctgtaaaat gaccgccatc ttttattgcg tcgcttaacc
    23941 ggcggtggtt gaataccgcc cggtcttaaa ggagagttta tcatgagtct ggatatcaac
    24001 cagattgctc tgcaccagct tatcaagcgt gatgagcaga accttgagct ggtgttgcgc
    24061 gattcattgc tggaaccgac agaaaccgtc gttgagatgg tggcagaact gcatcgggtc
    24121 tatagcgcca aaaataaagc ttacggcttg tttagcgaag agagtgaact ggcgcagacg
    24181 ctgcgcttac agcgtcaggg cgaagaggac tttctggcat ttagccgcgc ggcaaccggg
    24241 cgcctgcgtg acgaattagc gaaatatcct tttgctgacg gtggattcgt gctgttttgc
    24301 cactaccgtt atctggcggt ggagtatctg ctggtggcag ttctgagcaa cctgagcagt
    24361 atgcgcgtta acgaaaatct cgacatcaat ccaacccatt atcttgatat caatcatgcg
    24421 gatattgttg cgcgtatcga tttaaccgaa tgggaaacca atccagagtc cacccgctat
    24481 ctcactttcc tgaaagggcg agtagggcgc aaagtggcgg atttctttat ggatttcctc
    24541 ggggccagcg aagggttaaa cgcgaaagcg caaaaccgtg ggctgttgca agcggttgat
    24601 gatttcactg ccgaagcaca actggataaa gcagagcgac agaacgtgcg ccagcaggtt
    24661 tatagctact gtaatgagca gctgcaggca ggcgaagaga tcgaactgga gtcgctgtcg
    24721 aaagagctgg caggcgtcag cgaagtgagt ttcacggagt ttgcggctga aaaaggctac
    24781 gaactggaag agagcttccc ggcagaccgt agcacactgc gtcagttgac gaagtttgcc
    24841 ggtagcggcg gtggcttgac tattaacttt gatgcgatgc tgttaggtga acgcatcttc
    24901 tgggatccgg caaccgatac cctgaccatt aaaggcacgc cgccgaattt gcgtgaccaa
    24961 ttgcagcgcc ggacgtctgg cggcaattaa tcggttttgt gttctgaatg ccagatgcgg
    25021 cgtgaacgcc ttatctggcc tgcaaaaaat agcaataccg taatcaggca taatctgcag
    25081 atacaaaaaa ccccgccgga gcggggtttt ttacaactta ttcagcaatt aagcgcgaac
    25141 gaagtcgatg tgctgcagct tcggtttgta cgggtgacgc tgtacgtcct gagctttaac
    25201 tttgatttct ttaccgtcaa caacgatggt cagaacttcg ctgtagaatt cagctttagc
    25261 ttgcatgttc atgactttgt cgtgatccag ctcgatagcc agcggcgctt ctttgccacc
    25321 gtagatgatt gccgggaact tgttagcggc acgcaggcgg cggctcgcac ccttaccctg
    25381 ctctttacgt acttctgcgt tgatagtaaa catttctttc tctcttaaat taatcctgct
    25441 acaggcgacc cagcaacagg taagtgatct gctttgcgta tgcaaaagcg ggcgagattc
    25501 tatactcaat cgctttatac atcaatcaaa actaccatta accgcgtaat tcgtgcgccc
    25561 gacgaaaacg accttcgtaa tcgaacactt tttcacgcac ttgccagtac tgacctttca
    25621 tgcgggcaac gacaaaatcc gggtgtcgca ataaggcttg ctgggcgagg atatcggcgg
    25681 cggtgatcca gcgcagaggg atgcccggtg tgcgcgtatg cgggcggata aaaagctgct
    25741 cgaaggcagt tcgctgggcg ggcgtttgca gacggaaacg ctcactcaca tccgcgccgt
    25801 cttcatcgta ataggtgatt ttcaaccatt cgcctttctc gtcatgccca tgctgtaaag
    25861 acatgccgct acagcgtaat accagcgcgt ctttcagtcg tagcgccgct tttaacatat
    25921 cgtctggatc aaccagtacg gtgtcacatt cgcggcagcg gcgagcggca atatcgtttt
    25981 ccgcgttaca ttgcgggcaa tttttaaaac ggaaacggaa gtcacattgt tcgcgatgac
    26041 cgtcgtcatc ttcaaaccat ccctgacagc gacgaccaaa gtgttcaatc aatgtcccgt
    26101 cggcggtcgt tttcccccaa aaggtgttgg caaaaccgca ggcagggcag aaaacctgta
    26161 ccggaacgtt gtcacttttg ccttttggtg taccaacttc cggcgcgtag agatcgtgag
    26221 gattacccgc ataatcaaga attaagcaat cagtcttgcc cggagcgaga cgcagaccgc
    26281 gcccgacaat ttgttggtaa agactcactg attcggtagg gcgcagaatg gcgataagat
    26341 cgacgtgcgg ggcgtcaaat ccggtggtca gtactgcgac gttgaccaga tagcgaaaac
    26401 gctgggcttt aaaatcttca attaacacat cgcgctcagc gccgggggtg tcgccagtaa
    26461 tcagtgcagc atcttcggca ggcagtaatc ccacaatctc ttttgcgtgt tcaacggtgg
    26521 cggcgaaaat cattacccct ttgcgctttt cggcgaactc catgatctgg ctgatgatat
    26581 gtggcgtaat gcgttgctgt tttttcagct cacggttgag atcagcttcg ctgaacagcc
    26641 cgttactctg tgcctgcaag cggctgaaat cgtattgcac tactggcata tccagtcgtt
    26701 ctggcggcgt cagatagccg tgtttaatca tataacgcag cggcagctca taaatgcagt
    26761 cacggaaaag ggctttctca tcgccgcgta ccatgccgtg ataatggaac tggtagatcc
    26821 agcctttgcc caatcgaaaa ggcgtggcag tcagccccag caggcgtaag tggggattca
    26881 ctttagtcag gtgagtaagg atttgctgat actggctctc ttcatcgtca ccaatacggt
    26941 ggcattcatc gacaatcaac agcgaaaatt caccctgaaa ggcatcaaga ttacgagcga
    27001 ccgactgcac gctgccaaat accactttac cgtggctctc tttgcgcttt aacccagcgg
    27061 caaaaatatc ggcttccagc cccagcgcct gatactttgc atggttttgc gccaccagtt
    27121 ctttgacgtg tgccagcacc agaacacgac cacgcgccag ccgtgccagt tcggcaatca
    27181 ccaggctttt acctgcgccg gtgggcagca cgataacggc aggggtttta tgacgacgaa
    27241 aatggttgag cgtggcatcc acggcttctt gctgatatgg gcggagtgta aaaatcatgg
    27301 tctcgcgtag gtagattatt ctgctaatag tatgccatgg atctttttcc cttgagggat
    27361 gttattagac cgctatactg ggcagacaac agttcagatt ttcgggtcaa tgcccgacct
    27421 ccgtttttac aggcaaaatc acacacatgc gacttgataa atttatcgca cagcaactcg
    27481 gcgttagccg tgctattgcc gggcgtgaaa tccgcggcaa tcgtgtcacc gtcgatggcg
    27541 aaattgttcg taatgcagcg ttcaaactgc ttcctgaaca tgatgtcgct tacgatggca
    27601 acccgctggc gcagcaacat ggtccacgtt acttcatgct caataagcct cagggctatg
    27661 tttgctccac ggacgaccct gatcacccaa cggtgctcta ttttctggat gaaccggtag
    27721 cctggaaact gcacgccgca ggacgtctgg atatcgatac caccggtctg gtgctgatga
    27781 ctgatgatgg tcagtggtcg caccgcatta cttctccgcg ccatcattgc gagaagacct
    27841 atctggtgac actggaatca cctgtagctg acgatacggc agagcaattt gctaaaggcg
    27901 tgcagctgca taacgaaaaa gatctcacta agcctgcggt gctggaagtg attaccccaa
    27961 cgcaggttcg tctgaccatc agcgaagggc gttatcatca ggtgaaacgc atgttcgccg
    28021 ccgtgggtaa ccacgtggtt gagctgcatc gtgaacgtat tggcggtatt acgctggatg
    28081 ctgatttagc ccccggtgaa tatcgtccgt taactgaaga agaaattgcc agcgtcgtct
    28141 aacttttcca tgactttcag gagcccgttg tgaccacccg acagcattcg tcgtttgcta
    28201 ttgtttttat ccttggcctg ctggccatgt tgatgccgct gtcgattgat atgtatctgc
    28261 ccgcgctacc ggtaatttca gcgcagtttg gcgtacctgc gggcagtacg cagatgaccc
    28321 tcagtactta tattctgggc tttgcgttgg ggcagttaat ctacgggccg atggcagaca
    28381 gcttcgggcg taagccggtg gtgctcggcg gtacgctggt gtttgccgcc gccgcggtgg
    28441 cgtgtgcgtt ggcaaacacc atcgatcagc tgattgtgat gcgtttcttc cacgggctgg
    28501 cggcggctgc ggccagcgtg gttatcaacg ctttgatgcg cgatatttac ccgaaagaag
    28561 agttctcgcg gatgatgtcg tttgtcatgc tggtgaccac tattgcaccg ttgatggcac
    28621 cgattgtcgg cggttgggtg ctggtgtggc tgagctggca ttacatcttc tggatcctgg
    28681 cattagcggc gattctggct tcggcaatga ttttcttcct gattaaagaa accttaccac
    28741 cggagcgtcg tcagccattt cacattcgta ccactattgg taactttgcg gcgctgttcc
    28801 gtcataagcg tgtcctgagt tatatgctgg ccagcggttt cagctttgcc ggaatgttct
    28861 cgttcttaag cgccggaccg tttgtctaca ttgaaattaa ccacgtcgcg ccggaaaact
    28921 ttggttatta ctttgcacta aacattgttt tcctgttcgt gatgaccatt tttaacagcc
    28981 gcttcgttcg ccgcattggc gcgttaaata tgttccgctc ggggttgtgg atacaattta
    29041 ttatggcagc gtggatggtc atcagtgcgc tgctggggct gggattctgg tcgctggtgg
    29101 ttggcgttgc ggcgtttgtg ggctgcgtgt cgatggtgtc atccaatgcg atggcggtca
    29161 ttcttgatga gtttccccat atggcgggaa cggcatcttc gctggcagga accttccgtt
    29221 ttggcatagg ggcaattgtt ggcgcattgc tttctcttgc gacctttaac tctgcatggc
    29281 cgatgatttg gtcaattgca ttctgcgcaa gcagctccat tctcttctgt ctgtacgcca
    29341 gtcgaccgaa aaaacggtga tctattgcac aacgaggaag ctaaaaggct tcctttgttg
    29401 atgcatgtca accacaaatc tatcattccc ccgatatatg tttattttat gtaaaatcaa
    29461 tttatgtaaa aagtcacatc attgtagtta aaaaggttga gttagatcgc agaaacgggt
    29521 acatatagcc ccgcaaacgt gaccacgccc gcagatatta cttaaatcag agccatagag
    29581 gccacgcagg cgaggcatca atctttacga tctgtataaa gacggattgt tgatgatgtg
    29641 ttaaaattga tgtaaacaaa ttgtgaagtg aatgtacttc cggggaaaat aagtgacttc
    29701 attacaactc tcaatcgtcc atcgactgcc gcagaactat cgctggtctg ctggtttcgc
    29761 aggttcgaag gttgaaccga ttccgcaaaa tggaccgtgc ggtgacaaca gcctggtggc
    29821 gcttaaattg cttagcccgg atggtgataa tgcatggtcg gtgatgtata aactaagcca
    29881 ggcgttaagc gacatcgaag ttccatgttc ggtgctggag tgtgaaggtg agccgtgcct
    29941 gtttgtaaat cgccaggacg agtttgctgc aacatgccga ttgaaaaatt ttggtgtggc
    30001 aattgccgaa cccttttcaa actacaatcc tttttaagcg tcagctcaac gccagtagct
    30061 gacgcgtata ctcctgctgc ggtgtggcaa acacgcgcgc gcacggtcct tgttccacta
    30121 cttccccttg tcgcagtacg ataacctgat gacataacgc gcggacaacg tgcaaatcgt
    30181 ggctgataaa caaataggcc agttgatgct tttgttgcaa tgatttcaat agcgtcaata
    30241 tttgcgcctg taccgttttg tcgagtgatg atgtcggttc atcaagtatg atcagcgagg
    30301 gcttaagaat taatgccctg gcaatcgcaa tacgttgtcg ctgaccacca gagaactccg
    30361 ccggataacg gtggcgtgtt tcaggatcta atcccacttc atgcatcacg gctatcactt
    30421 gttgttcgcg ttgtgcggca gaaagcgtcg gctggtgaac ccgtaagcct tcctcaataa
    30481 tctgcaaaac gttgagtcgt gggttgagcg aggagtttgg atcctgaaat accacctgaa
    30541 tgcgatgacg aataggtaac agctggcggc gatttaaatt ttgcagtggc tgaccgtcaa
    30601 agatgatgct gccctgagaa ttaatcagtc gcagcagcgc cagtcccgtc gtacttttcc
    30661 cggaaccgga ctcgcccact aaacccagtg tttcacccgc tcgtagcgta aaactgatgt
    30721 ttttcaccac cacattatga tccacaatgc gcttcaaaat ccctttgcga atggggaagg
    30781 caacctgaag ctgttcaaca tccagcaacg ttgaggcagg ttctggcaac ggcactggat
    30841 cgcctgacgg ttcactgttg agtagctttt gtgtgtaagg atgagtgggt gatgcaaata
    30901 gcgtagcggc gtaattttgc tcgacacagc gaccgttttg cattaccgcc acgcggtggg
    30961 ccagttttct gacaatgctg aggttatgag taataaacag catgcccata ttcagctcgc
    31021 cttgcagttc gcgcaacagc tgtaaaatct gcgcctggac agagacgtcc agtgcggtgg
    31081 tcggttcatc ggcaattaat aattccggtc gcgttaacag cgccatcgca atcatcaccc
    31141 gctggcgttc gccgccggag agctgatgcg gataatctgt cagccgtttt gccgcctggc
    31201 ggataccaac gcgatcaagg cagttaagaa tttcgccacg agccgcttcc cgacgcatcc
    31261 cgcggtggag tgaaagcact tcataaagct gtttttccag ggtatgcaat ggatttaacg
    31321 acaccatcgg ttcctgaaaa atcatggcga tcttattacc gcgtacaccg cgcaacgttt
    31381 gagcgctgac atgaagcagc gattcgccat gaaaacgaat atcgccggag agatattcaa
    31441 ccggcgggga agggagcagg cgtaaaattg acagcgcggt aacgcttttg cctgaacctg
    31501 actcacccac cagcgccagc gtttcgccag cctcaatctg tagtgaaaca tcattgacta
    31561 ctgtacgtac ggtttgctga tggcgaaaac ccaccgacaa attttcaatc gctaacagag
    31621 tttgcgtcat gctacaccgc cttattagga tcaaatgcgt cgcggacggc ttcaccaata
    31681 aagatcagca aagacaataa tatcgccacc gacaagaagg cggtgatccc aagccacggg
    31741 gcctgaaggt tatttttccc ttgtaacagc agttcaccga gtgacggtga accgagcggt
    31801 agaccgaagc cgaggaaatc gagcgaggtc agggtggtta tcgaactaca taaaataaac
    31861 ggtaaaaagg tgagggtcgc gaccatggca ttaggcaaca tatgacgcag gatgatactg
    31921 cgatcgctga cgccaagcgc ctgtgccgca cgaatgtagt cgaaattacg agtacgtaaa
    31981 aactccgccc gcaccacgcc gaccagactc atccagccaa acaagacagt aattgccagc
    32041 agccaccaga agttaggctg tacgacgctg gaaagtaaaa taatcaaaaa cagcgtcggc
    32101 atccccgacc atacttcaat aaagcgttgt ccccagagat cgactttacc gccgtaatag
    32161 ccttgtagcg cccccgccag cacgcccatc acgctggaac agagagtcag catcaggcca
    32221 aacagaaccg agatccgcgt gccatagaga atgcgtgcca gcacatcgcc gccgttggca
    32281 tccgttccca gccagttttg ccgggagggg ggagaaggga agggcttatc ggtagcaaag
    32341 ttgatactgg tagcaccaaa gcgaatcggt gcccacagta cccagccgtt attttccagc
    32401 cgttgtttca gccacgggtc ctgataatca gcctgacttg ccagcgggcc gccaaaatcg
    32461 ctttcgctgt agtttttcaa caacgggaaa taccaactgc cgtcataacg taccagcaac
    32521 ggtttatcgt tggcgataag ttcagaacac aaactcaaac caaacaaaac gaggaaaatc
    32581 cataacgacc agtagccgcg acggttatgg cgaaaacgcg cccaacgggc ctgattgacg
    32641 gggctgagtc gcgacattaa cgtccctcaa aatctatacg cggatcgacc agcgtatagc
    32701 tgatatcact gacaatattc agcagcaggc caatcagggt gaaaatatac aaggtaccaa
    32761 acattacagg ataatcgcgc gagacggtcg cttcgtagcc cagtaagcca aggccattga
    32821 gtgaaaacat cacttcaatc agcagcgagc cggtaaaaaa catgctgata aacgtcgccg
    32881 gaaaaccggc aatcaccagc agcatggcgt tgcggaacac atgtttccag agaatatttt
    32941 tttcacttac ccctttcgca cgcgcggtca ccacgtattg cttgcgcact tcatcaagga
    33001 atgagttttt ggtcagcatg gtcagcgccg caaagccacc aatcactgtc gccagcaccg
    33061 gcagcgtgat atgccacaga taatcggtga ttttctgata ccacggcagc gaatcaaagt
    33121 tagcggaaac caggccgcgt agagggaaca ggtcgaaata gctgccgccc gcgaagaaga
    33181 caatcagcag gatggcaaac aaaaaggccg gaatggcgta gccgatgatg ataaatgcgc
    33241 tactccagac gtcaaagcgg ctcccattat aaacagcttt gcgaatgcct aacggaatcg
    33301 acaccagata gataatcagc gtgctccaca atccgagggt gatggaaacc ggcagactgt
    33361 ctttaatcag cgtcagcacc gaggcgctgc gaaaaaggct atcaccaaaa tcaaagcgga
    33421 tgtagtccca gatcattttg aagtaacgtt cgtggatcgg cttatcgaaa ccgtagcgat
    33481 gagtgatctc ggcgatcact tctggatcta atccgcgtcc gccacggtaa ttactgtcgc
    33541 tgatattgcc gacgcccgtt tgcgcatggc tggcgcgaat accttcaccg cctgcgccgg
    33601 gtaatactcc ggcattacca aactcaatgg cggcgattgc ctggtcgacc ggaccgccag
    33661 gcgcaatttg cacgatgaaa aagttgatag tgataatcgc ccataacgtt gggatcacca
    33721 gcaacagacg gcgaatcaga taagcgccca tctactctcc ctgttgtctg gctgacggca
    33781 gtttggccgc tttattaacg tcataccacc aggtatcgat acccaggctg taaacagggc
    33841 gtacagcggg ttgggagaat ttatcccacc aggcgagacg gtcttccgcc atgtaccaca
    33901 ttggcagcat gtaataattc cacgttaata cgcgatccag tgctcgccct aacggcagta
    33961 atttttcttt atttccctgc gcggcaataa tttgattgat cagcgagtcg ataaccggac
    34021 tttgcacgcc gggggcatta taagtggaat tgatatattc cgatgaccag gaaatctgta
    34081 aatcggaact gggccacggc atcgcccgcc atacgcgcgg catcatgtca tagtcgcgac
    34141 tgcgcatgcg gttggtgatt tgcgagttat ccaccttgcg aatgtccatg ttgataccca
    34201 gccgttgcag gctgtgctgg aacggcaata cccactgact attgctgctt gcgggaagca
    34261 ataattcaaa gctgagtggc tgacccgttg tggcattaac gcgttgctga cccttcagca
    34321 cccagcccgc ttcgttgaga agtttgtcgg cttttaacag gttgtcacga tcgtagccat
    34381 cgcctttaga taccggcggc tggtagattt gtgtgaagac ttctgacggt agatcttttt
    34441 tcattggtgc cagaagcacc agctccgcgg cgtcggggta atttctggcc gcgtattcgg
    34501 tattctgaaa ataactgttc gtgcgactcc aggcattgta aaacaacgcc ttgttcatcc
    34561 attcaaagtc aaaggcgagg gtgattgctt cccggacccg gcgatcgctg aataccggac
    34621 gttggatatt aaacgccagc cagcgcgtat cctgggctga ttcgttcttt tgctcatctt
    34681 tgatgatgta ttttttatcg aaatttttac cggtataacg cgtggcccag tttttagcgt
    34741 cgttttccat acgcaaatca aaggcacctg ctttaaacgc ttcaaaggcg acattatcat
    34801 cgaggtaata atcgtagcga atggtgtcga aattccagcg tccacggttt accggtaagt
    34861 ttgctgccca gtaatctttc acgcgggaat agacaatatt ttgccccatt ttccaggacg
    34921 taattcggta cggaccactg gcaagcggag gcgtggcgag cggatcgcta agtttgtgat
    34981 ccttccagta cttttctgga aataccggca gcgaaaacag actcaacata tcttctttgc
    35041 cgggtttagc taactcaatg cgcacggtta acggcgcaat ggctttgacg gtggtgcctt
    35101 tgtagaccag acgaaattgc ggcacgcctt cggtcataaa tttttgaaaa gtaaactcta
    35161 catcgcgggc agtaatgggc gaaccatcat gaaaacgggc gcgcggatta atagcgacct
    35221 ccacccagga ataatcgtca gcatagcgtg cgctttcagc aatcagcggg taataactgc
    35281 ctggttcgtc atcggaagtc gtaaatagcg tgtcgtacag ctgctcggtg cgtgcgcccg
    35341 ggttgccgcg cagtgcatag cggttgaaat tatcgaaggt accgagggct gacaacgtta
    35401 tctgcccacc ttttggcgcg gcggggttca cataatcaaa atggttgaaa ttaaacgcgt
    35461 accggggttc gcccagcacg gcaaaggcat agctttcctt gatagcctgc gcctgcgcac
    35521 caaaggtgaa cagagcgata aacagcagca gtatgcgcac aatcatctgc aacggatttc
    35581 cttttactca tcattagccg caatccggca tgctttcact atgaataata agggacgtaa
    35641 gcacacctta ccactgcggc gtatacggtt tcttcagcca gcgaacaaaa tcgtccagcg
    35701 gtaacgggcg actaatccag tagccttgca tgaaattaac gccgcgttcg cttagccatc
    35761 gcgcttgttc cggcgtttcg accccttcag caaccgtcag catattgagg cgtttcgcca
    35821 gcgtcagcac cgcgtcaagt acgggtgaag tgatcgtttc cgtaccgatg gcgttgataa
    35881 atccacggtc aattttcaga taatcgagcg taaaacgctc aagatagata agcgcgctgt
    35941 gcccggtgcc gaagtcatca atagcaattt ctacgcctac cgagtgcagc caggcgaaga
    36001 gttgtgtggc ttcttgctct ttcagcatat cgcgctcggt aatttccagc acaatctgga
    36061 aatggtgtgc gggtagggaa gtgagcagtt tctggatatc tgctttaaag ctttcgctgt
    36121 gcagatggtc cggcgcaatg ttaatgccaa atttgacgcc taccggcagc actttttcta
    36181 attctgcggc atcgcgggca attaactcaa acagatgctg agtcagcggc acaatcatct
    36241 tttgcgattc ggcaaagtta atgaaggcat ccgggggaat ttctcccgcg acaggatgcc
    36301 gccagcgtag cagtacttcc aggcccgtta ctcgcaaagc ttgtgtatcc accaccggtt
    36361 gatacgccac gtaaaattgt tcgcgcttga tggcggtcat gatttctctg ccggggcgca
    36421 tacgtacgct catcaggtaa tagcagagca ggccaacgac agttccactc atgccgccca
    36481 gtaaaaatgc gtaccacaca tcgttccatg tccagtcatc tgcataaagc cgaacggtca
    36541 gaggaatgcc cgcaatttta gtttcacgga ctggcatgtc ggttaattcg ttaacgttca
    36601 tcaaacgtga agaaaaggta gatagcgcag tattgccaat aatgagggcg acgccatcgt
    36661 aatcttcctg ccgtgaacta taaaagagtg aaggcgtcag gttgagattc agagcggcaa
    36721 agacgccgct atttttcagc aaagggttgc gataccagat gacgattgcg ggtttgttcg
    36781 gcaccatcgg cgtgccgggt aagatcgcca tatcgacatt tttattaatg tcgagcgccg
    36841 gaatcaattc attgagtgga atgtccatct caccggtcgc agatgaacag aatgtttttt
    36901 tatctttcac cagcacaaac gtacggacat tcatgctaaa cgccgcgcgg gcggtcagtt
    36961 cggggttagc ctgctggcag gtatctaagg tcagcggctg gagccggtca gtagtggatt
    37021 tcaggtcggc aaaataggta tcgagatact tttgtacgtc ggtaatcagt gtgtcgtatt
    37081 tgacttcgtg cttatgccag gccactaaaa actgaaggca actcaccagt atcgcaatca
    37141 tcacgcctgc aacaatgcag gtaagcagga gcttacgtcc agaattggga gcgcgtatga
    37201 acatgattcc gttaatttcc tgaaattacg acaacattta aaagataaca atgaccggac
    37261 tatagcctgg atcaagacgc ggaaacatca ttttctctga tgaatgaaaa caaaaaagca
    37321 ctgcctaagc agtgctttca gttaactcgt taggatagcc aagggattgc atccaaacgg
    37381 tttattagct gcggctgaga acccggcgtg cttcgttgta acgcttcttc cagtacggtt
    37441 cattcatgct ggaaataata acaccactgc tggtggaagc atggacaaac tgattgttgc
    37501 cgatataaat accgacatgg cgtcccgttg aaccggcacg gaacagaact aaatcacccg
    37561 tacgcaaatt actgcgggaa acagatttac ccatttcctg ctgttcgtaa gtcgaacgcg
    37621 gaagttctaa gccaaattgc tcacggaatg tacgctgtac gaaaccagaa caatcgatac
    37681 cttttttagt gctgccgccc agacgataac gtacgccttt ccagtcagca tactgatcca
    37741 taattcgcga ttttacgtcg acattacgaa ccaggttttc aaattcatcc tgagaagctt
    37801 gcagtgatga tgtttcacta cccactgcac gtgtctcagg atgcatattc tttgcggtgt
    37861 tatttgcact acatgcagaa agcagaaccg ctactgcaat cgcggggatc ccgcgcaaga
    37921 tatatctcaa aatcggttga gatttgacca tgttgtttgt tttcccttga agtccttaac
    37981 gacaaatatc gttataaaaa aatgccaagc gacacgagac taattacctg ggcatgttga
    38041 aacaattcct taaggccaaa tctgtggact aacgcacaaa attatatgct gtgaggataa
    38101 tctaccgccg aggcaaaacg agttcgagat tacccgattg gtgcggtctt ggcgagcggt
    38161 tttacatgat tttttataag aaaaagtgat acagaaagtt aataagcggg attggtcaat
    38221 taaagtacga ttgagtgaaa cgcatgcaat ccattcattt acttagagaa tgatttttgc
    38281 gcgcgatgga tgaaagcacg gtgcgcatca tcaggattat cctcactata aaaataagcc
    38341 tgatgatgtt aattactgtg agttatttgt tttggaaatg tttgtttttt cccggtagtg
    38401 atttgtcaaa aaaggtgatt aatcgatcac ttaatggcgt cagcaacacc cagggcaaac
    38461 cgatcaatat cacggtcatc gaaccgacaa tgatgtcagt aaaccagtgt gcgccaatca
    38521 ttactctggg aaatgcaaaa accacaaaaa taataagggc gataaggcct gcaactttgc
    38581 cgaaataacg ccacatgaat gccgaaaaaa taagcagcat catgccgtga tcgccgggaa
    38641 agctatctcg tgaggcatct ttcgtgggaa cagagagcag ttcgctgacg cggttaatat
    38701 cggtaaaagt caatgttggg ctggcccgtt ttacaggaat taatgcctga cccagctggt
    38761 ttaatagcac tgcagttaat agcatgacca gacccataat cacgatacgt cgtctgccag
    38821 gggcgttttc tttcagccag aaactcagca tcaacattcc catcgccagc agagaacaac
    38881 cgtcgaaagc gcgattgttg gtcaatgcaa ccagccacaa aaaggcctta ctttcgacca
    38941 gtttctgatt aaagaaataa aaaatatccg catcaatcgg caaccagaat ccatgattaa
    39001 cgggaatata ccaggaaaga aacagcgcga ggccgacaat attcaacaac actatttgcg
    39061 gcaaattttt aatcatattt tctctaatta tcttactgaa aaacgcaggc aaccttacgc
    39121 ggtagtcgct aaacgaagct tcaacagcgc gctctgcaaa gcattccagt cagcttcgct
    39181 gctggaaatc agctcaatac ggctgtccgg tggcgcaacg ttttgcgttt caatgtgcag
    39241 gtcatcgccc tgacggttga ttcgtaccag cccttcggga atacgcagca cgcctttgac
    39301 gcgttccacc ggtgcaagtc gcgcccattc cagaatgcca atggtgtcga ataccgtatc
    39361 agcgtcgaat atccagccgc aggcctgata tccttgcccg ctgttcagac tgcgacgcca
    39421 gcgttgatgc tctggcaggc ttaacgctgc taaccctttt ttcacgacat gctgatgaga
    39481 atgcgcggcg ctggcgggca actcggctaa attgcgacgc ggcaaatcca gaagatgacc
    39541 gtcaactttc ccatgctcac tgtgaattaa ttgtcgatcg ccaccatttt gctgccacca
    39601 acgctgtagc gcttgctcac tttcgggcgt cgcacggtcg gatttattgg cgacaatgat
    39661 gtctgcggca gccagctggt cacggaagtt ttcattgctg gcgctttttt cgtccagcag
    39721 caagcgcgga tcgagaatgc acaaggtggc gcgcagatct atccacggtt catagactgg
    39781 tgcggttaac agatcgagga tctgtttcgg atggcccagc ccggtcggct ctatcaacaa
    39841 gcggtctggt tttccctgac gcagtaaggt attcaaccct acctgcatgg gtaaaccatt
    39901 aacgcagcac atgcagccgc cggggatctc tttcagcaat gcgccgctat cggcgagcaa
    39961 agcaccatca attccgactt ccccaaattc attaaccagt accgcccatt tttcgttggg
    40021 atctttatgg gctaacagat gaagaatcga cgtggttttc ccgctgccga gaaaaccggt
    40081 gatgaggttg gtcctggtca caacggctcc gcaatactct ggtaagttaa ctgagcgtta
    40141 atcctggcta aaaaaggcag aaaagagaag gggagagcgg acgaaggtga gcttcatccg
    40201 ctttaaccgc agaaatcgtt aaattttgag ggtgtttaat agcgcctgat gtgcgccgaa
    40261 ttgtacgatt tgttgccagg cttgttcgat ggcctgcaca aaatgcgggt tatctggcag
    40321 atcatcaccg aaaacttcac gcagggaaag cagggcggtt acgcgttgtt cactgctgct
    40381 gcccgcaaca agttcgcgaa ttttatcgct aagcggatcg cgaacatcaa tggcatttcc
    40441 ggcatcatca acgccgctga cgtaacgcat ccagccagca acgcctaatg ccagcaacga
    40501 ccagtccgtt tcgcacccca gatgtatgcg aatacctgcc agcatacgtt gcggtaattt
    40561 ctggctacca tccattgcga tttgccaggt cttatgtttc agcgccggat tagcaaaacg
    40621 tgcaattaac ttgtccgcat attgtgttaa atcgacatct ttaatttgta gtgtcggcgc
    40681 ttgctcattc agcattaatg ttctggcggc atggcgaaat gcgcgatcct gcatgcaatc
    40741 actgatatgg gcgaatcctg aaaggtaacc cagataagcg agaaaagagt ggctgccatt
    40801 aagcatacgc agtttcatct cttcccatgg caggacatca ttcaccattt gtacacctgc
    40861 gacttcccag gcaggacgcc cagcgacgaa gttatcttcc accacccact ggataaacgg
    40921 ttcgcagcta atcgcgcagg gatcatttac ccccagatgc tggctgattt ctgccagtga
    40981 ttcgtcggtt gcagccggaa caatgcggtc gaccatggtt cccggaaaac tgacgtgttc
    41041 ttttatccac ccggcgagtt ctggcgaacg tttttctgcc attcccagca ccgcgttttt
    41101 caccacatgg ccattgtcgg gaatattgtc gcaggagagc acggtaaacg gtgtaaggcc
    41161 tcgctcacgg cggcgtttca gtgcttcgac gagaataccc ggtgcggagt gaggttcttc
    41221 aggggtttgt agatcgtgaa taatccgcgg attactggtg tcgagtgcac cggtggccgg
    41281 gtcaatacaa tagccttttt cggtaatcgt cagggaaaca attgccacct gtggctcgca
    41341 aaatttctca ataattgccg ctaaggaatc cagttttgca ttaaggcatt cgtgaacggc
    41401 accgacaatt atcacctgat tgccgtccgc acctttttcc agcacggtat ataaatggtt
    41461 ctgtgcgcgg agctggctca tcagttgatc accgctgaac aagctgattt cacagatccc
    41521 ccagtcgccg ccctgggcat tcagcacacg atcggtcagt aacgcctgat gagcgcggtg
    41581 aaaggcgcca aagccaaaat gaacgatacg tgattgcaac tgctggcgat cgtagcgtgg
    41641 agcgtgtata tgatgcggga gcgtaacgga ggcaattgtc ttcataggag tcacgtctta
    41701 gtaacgataa attaacaatg acagtgtaaa gttatatgac agcaattgaa attggtctgg
    41761 attgttaatg cgatgaagtg tgagggcgat caatcaacga gtgaaaattg agggcgtaag
    41821 gtgttcagga agcgcaaaat agaagattgg tataataaat taaaaaaggg acagcctgag
    41881 ctatcccttt tctgcatgcg gctgaagtta gtcagcacgc cccatataac ggcgttcttc
    41941 gatatggata cgaatttttt cgcccgggct taagtattct ggtacctgaa tcaccagacc
    42001 agtgctcaat gtcgccggtt tgttacgggc gctggcagat gcccctttga tgcctggtgc
    42061 ggtttcaacg atttccagat caacggtctg cggcagctca agcgccagca gttgaccgtc
    42121 ccaggtcagc acctgcatgt ccggcatgcc gccctctggc ataaacagca actcttcttc
    42181 aatctgatct ttggtgaagg tatacggggt atagtcttct ttatccataa agacatattc
    42241 gttgccatcg acataggaga agtcaacgta acggcgggtc agcgtcacgg tgtcaacgat
    42301 atcatcacct ttgaaacgct cttctacttt cagcccggta cggacatcag aaaaacgcat
    42361 tttgtacagc gtagcggcac cgcgggcagt gggcgactga atatcaatat ccttaaccag
    42421 cagcagtttg ccgttgtaat tcagtaccat acctttttta atttcgttcg ctcttggcat
    42481 cgaaaaattc ctgtagtcga aaatgtcaaa aatatcgcga caaagtactc gcgacgggcc
    42541 tttcgggcaa gtggtattcg cacttttgct ggtgcaaaaa aggtggtact gtgcgcgctc
    42601 atcaatccgg tggttaacct taagagaaca acgtatgaaa tgccgtccgg gttgcggggc
    42661 gtgttgcact gccccttcaa tttccagccc tattcccggt atgccagatg gcaagcccgc
    42721 caatacgccc tgcattcagc ttgatgaaca gcaacgctgt aaaatattca cctcgccact
    42781 gcgaccgaaa gtctgtgcag gcttacaagc cagcgctgaa atgtgcggta actcgcgtca
    42841 gcaggccatg acctggctta tcgatctgga aatgctgacc gcgccttaaa catctttaat
    42901 ccgcagtaag caaaacagag tggcgataat catcaccatc gcaaaccaga acacagcgtg
    42961 ataattccag atctcggcga cgatgcccgc cactgatcct gcgatgatcc agcccacgcg
    43021 cgacgtgttg gtatagagcg tggtggctga acccgcctga ccgggcatca gatcctgaaa
    43081 atagagcatc ccgatgccgc ccagaatgcc aataaaaata gcatttagca gctgcaagcc
    43141 caacagaatg acaggtgaat gcgccatcag cattcctgcg taaaaacaga cgccacccac
    43201 ggcagcaacg cgcattaaga aacgcttacc cagacgtttg gcgaaatatc cggcaatcaa
    43261 catcgtcggg atttccagcc cggcggcggt ccccatcatc acaccggcca gtttctcggg
    43321 aagatgcagt tcgttgataa taaatagcgg catgttgatg atgtacaggc tgttcgagcc
    43381 ccacatcaat gtacaaatga caaacagcag cagcgtatcg cgacggttac ggcgcggcgc
    43441 ttcgatcgtg ccggtcgcca gcggaagctc tttttgcatc gacggtaaaa acagccacac
    43501 catcacaccg caaacaataa acgctaccgc tgcgctcaga tacattaccg taaagctgaa
    43561 acccatcgct aaggcataag ccagcggtgg gccaatgacc catgccagtg aaacctgagc
    43621 gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat gttcacgggc
    43681 aagggcaaac atttgcgggt tagcggtcga gccaaagctg ctaagaaaga cgccaacgaa
    43741 tagcaaaaca aagtagttgc gattccaggc aaaaagggtg caggccagca cgcctaacag
    43801 gcagcaaaag acaatcagcg atttgcgatc gccgcgctta tcagagcgcc cggcgagaaa
    43861 ctgacttacc agaatcccaa tgacagcgct gccggtaaag aagaatccca ccatcgccgg
    43921 acgagcatgt acttcatcag taagaaaaat actgagtgtc ggggtttgca gagcgcccgc
    43981 aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa acgatttcgc
    44041 gctggagact gcgggggagt tatgcatgct ggtcgatcac tggaagagga agacgcggag
    44101 tttacgctgc ttgtcagaaa ataaacagac agcgaaatca aaagcgccac gaataatcaa
    44161 aatgccattt cactgttgct gattggctgc tgacgctgct gaaatgaaac tgctgataac
    44221 ggattttccc atcagcaatt aggaaaaatg gcaaaaaatt gtgcagcaca tcaaactttt
    44281 gctcataact ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct ttcttgaaac
    44341 gtttcagcgc gatcttgtct ttaaccctaa gccaggttgg cgcttttttt ctcatagagg
    44401 ctgaatcgtt tcaattcagc aagagaggag aactatgttc cagttatccg tacaggacat
    44461 ccatccgggc gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg ctgcggcgct
    44521 ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc gcgaacagca
    44581 aacctcaacg ttcctcggca atggtattgc tattccacac ggcactaccg acacccgcga
    44641 tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca cctggggtga
    44701 cggtcaggta gcgtacgtgg caatcggtat tgctgccagc tcggatgaac atctgggcct
    44761 gctacgccag ctgacccacg tactgagcga tgattccgtt gctgaacaac tgaagtcagc
    44821 accaacagca gaagaacttc gcgcattact gatgggcgaa aagcagagtg agcagctgaa
    44881 gctcgacaac gaaatgctga cgctggatat cgtcgccagc gatctgctga ctcttcaggc
    44941 gctgaacgct gcgcgtctga aagaggcgag ggcagttgac gccactttcg tcaccaaagc
    45001 catcaatgaa caaccgctga acctcggaca gggtatctgg ctgagcgata gcgccgaagg
    45061 caatctgcgt agcgcgattg cggtaagccg tgcggcaaat gcttttgatg tggacggcga
    45121 aacggcagcc atgctggtga gtgtggcgat gaatgacgat cagcccattg cggttcttaa
    45181 gcgtctcgct gatttgttgc tcgacaataa agctgaccgc ttgctgaaag cggatgcggc
    45241 aacgttgctg gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt taagcgcgga
    45301 gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc tggtcaatac
    45361 cattaaacaa tttaacagtg atattaccgt gacaaacctt gatggcaccg gcaaaccggc
    45421 aaacggacgt agtctgatga aagttgtggc acttggcgtt aagaaaggtc atcgcctacg
    45481 ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa gcaatcggcg acgctatcgc
    45541 tgctggtctt ggggagggcg cataatgagc agacgtgttg ctactatcac ccttaatccg
    45601 gcttatgacc ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa cctggtgaaa
    45661 accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt aaaagacctg
    45721 ggaattgatg tcaccgttgg cggcttcctc ggtaaagaca atcaggatgg ttttcagcaa
    45781 ctgttcagcg agctgggcat tgccaaccgt ttccaggttg tacaggggcg cacccgaatt
    45841 aacgttaagc tgacggaaaa agacggcgaa gtgaccgact tcaacttctc gggttttgaa
    45901 gtcacccccg ccgactggga acgctttgtg actgattctc tgagctggct cggtcagttc
    45961 gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc gttcaccgac
    46021 tggatgactc gcctgcgtag tcagtgtcct tgcattatct ttgatagtag ccgtgaagcg
    46081 ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg cgagctggaa
    46141 atctgggcag gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc acatgcgcta
    46201 cgtgaacaag gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc gctttgggtt
    46261 aatgcctccg gcgaatggat cgccaaacca ccgtcagtcg atgtcgtaag caccgttggc
    46321 gcaggggatt ctatggttgg tggcctgatt tatggcttgc tgatgcgtga atccagtgaa
    46381 cacacactgc gtctggcgac tgctgttgca gccctggcgg taagtcaaag caatgtgggt
    46441 attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca accttttaac
    46501 tgacagcagg agaggcataa tgaaaacgct gctgattatt gacgctaatc tcggtcaggc
    46561 acgcgcctat atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa aactggaaat
    46621 catcgacaat ccgaacgacg cagaaatggc gattgttctc ggtgattcca tcccgaatga
    46681 cagcgcgctg aacggtaaaa atgtctggct gggtgatatt tcccgggcag ttgcgcaccc
    46741 tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc cggtcactgc
    46801 gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg cttgcccgac
    46861 tggcgtagca cacaccttta tggcggctga agccattgaa accgaagcga aaaaacgtgg
    46921 ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa tcactcccga
    46981 ggaagtagcc gcagcggatc tggtgattgt ggcggcagat atcgaagtgg atctggcgaa
    47041 atttgctggt aaaccgatgt atcgtacctc taccggtctg gcgctgaaga aaaccgcgca
    47101 ggaactggat aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca aagctcaaac
    47161 ggcgacctct gaaggtaaga aagagagtgc aggcgcatac cgtcacttgc tgacgggcgt
    47221 ctcttacatg ctgccgatgg tcgttgcagg tggtctgtgt atcgcgcttt cttttgcttt
    47281 tggtatcgaa gcgtttaaag agccgggtac gttggcagcg gcgctgatgc agattggtgg
    47341 tggttcagcc tttgcgctga tggtgccggt actggcaggt tacattgcct tctccattgc
    47401 cgatcgtccg ggtctcacgc cgggtctgat tggcggtatg ctggcggtca gcaccggttc
    47461 tggcttcatt ggcggtatta ttgcgggctt cctggctggt tacattgcga agttaatcag
    47521 tacgcaactg aaactgccac agagtatgga ggcgctgaaa ccgatcctga tcattccgct
    47581 aatttccagt ctggtggtcg gtctggcgat gatctacctg atcggtaaac cggttgctgg
    47641 cattctcgaa gggttgactc actggctgca gaccatgggg actgcgaatg cggttctgct
    47701 gggggcgatc ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa ataaagcagc
    47761 gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg cggcgattat
    47821 ggcggcaggt atggtgccac cgctggcaat gggcctggca acaatggtgg cgcgtcgcaa
    47881 attcgacaaa gcgcagcagg aaggtggcaa agccgctctg gtactgggac tgtgctttat
    47941 ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc cgtgctgtat
    48001 cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac tgatggcacc
    48061 gcacggtggt ctgtttgttc tgctgatccc tggcgctatt acgccggtat tgggttacct
    48121 ggtagcaatt attgccggta cgctggtggc gggtttggcc tatgccttcc tgaaacgtcc
    48181 ggaagtggac gcagtagcga aagcagcgta ataaaaggtg tgttacaggg cagaaatttc
    48241 ctgtcctgta acatttgatg agcgcaaaga gaatgaccgg tttgtgaatg taattttatg
    48301 aatttaaatg gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca ctcaggttat
    48361 ttcctcaagg gcagattatt ttctgccctt ttttattgtt ctgttctgtg catcccgtca
    48421 caaattcctc gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct
    48481 gcccattttg cctctctggc ggtaatttat tttacgtaaa acatgcatac tatgagcaca
    48541 tgtttaaata tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga
    48601 cagggttggt aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt
    48661 ataggttcgg cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat
    48721 tcaaatccag gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac
    48781 ctggcgttgc tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt
    48841 caatcgctgc taacgcaaac caatcaatct ggcgtttatg tcgataaatg cctgattgtg
    48901 ccgggagaaa atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt
    48961 gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc acagcacggt
    49021 gaatttattc agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg
    49081 gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcggcatgg
    49141 aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctcaagcc aaaccgcctt
    49201 gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg aagatgtggc aaaagttgct
    49261 gcctggttcc atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt
    49321 tattacagcg atatcagcgg tgaaagtggc tggtctgcgc cgatcaaaac caatgttatt
    49381 aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga
    49441 atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt
    49501 gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca
    49561 gaatgtctga attaaaaatt tcccctgaat tattacaaat ttccccggaa gtgcaggacg
    49621 ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc
    49681 cgttcccaca aaatgcccag accgcaattg aagttgaaga aactattcgt aaacagggcg
    49741 ctgtacctgc cacgatcgcc attattggcg gcgtgatgaa agtggggtta agcaaagaag
    49801 aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac
    49861 cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg
    49921 cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg
    49981 cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg
    50041 ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa
    50101 ccttcggtgt gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgca
    50161 ccagcccgtt tgacgtcagc attcgtctcg acagcgccag cgaaattgcc cgtgcaatgg
    50221 cggtgaaatg gcaaagcggt ctgaacggtg gcctcgtggt agcgaacccg atcccggaac
    50281 agtttgctat gccggaacac actatcaatg cggcgatcga tcaggcggta gctgaagctg
    50341 aagctcaggg agtcattggt aaagaaagta cgccattcct gctggcgcgc gttgctgaac
    50401 tgaccggcgg tgacagcctg aaatccaaca tccagctggt gttcaacaac gccattctgg
    50461 cgagcgaaat tgccaaagaa tatcagcgtc tcgcgggtta atttctgacg ggcagaaagc
    50521 cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt taccggcatg
    50581 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa
    50641 tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac
    50701 tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag
    50761 tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct
    50821 tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta
    50881 tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
    50941 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc
    51001 tcaacatcag caaaatcgaa tcttttgttg cggttactac tattttcctc gggcaaaatg
    51061 agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg
    51121 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
    51181 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatccctggc gggattttgt
    51241 ttgcacgtat tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt
    51301 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
    51361 taaaaatcgc cgctggtgtg gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
    51421 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
    51481 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
    51541 ccaatcttgc gggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc
    51601 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc attgcgatta
    51661 tctcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
    51721 tttcggctat ttcgccaaaa cgcgcgccgg aaatcgccca gcttggttta cgggcgctgg
    51781 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
    51841 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
    51901 tattgaaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
    51961 cgccagtcct gacaactgct ttctgttttt aatgagatac cctttcttgc ttttaattaa
    52021 taaaccgtcg tgaatgaact gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
    52081 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcctgcg agagtaatat
    52141 aaatgctgcc aggcgattca ctaacggaaa tgattgattc tgagttaaag aaacaatatt
    52201 acgataattt ttatgactta aggtgacgca gagttttcgt aaaaatagcg tgtcgtttaa
    52261 taacagcgga cggtaatgtt tcatagggag cgcaaggcac caacactctt caatagcctg
    52321 caccgcacgc ggttcatggt ctttatcgat taactcaatc tcgccaataa aacagggggc
    52381 ggcaaagaaa tcgatcagcg acacgcgacc attagctagc gtggcgtaaa gcctggcgcg
    52441 gcctcgcgtc aggtaaaaca gccaggacgg ctgttgccct tcctgcacaa tgtagtcacg
    52501 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttaaaaagc aatccttaaa
    52561 cgcggattca ctcatcaatt gctgcttaag atcattattg tggatttctt tcacgatgtt
    52621 tatccgctat gagatttctc atatttagca tacgtacgct gttgttaaga ttcaatcatc
    52681 ggcccggtcg aattttcttt gctgtagaaa aaataacgca actggaaaca gaggaaataa
    52741 acaatggaaa agagaaaaat tattctggat tgtgatccgg gtcatgatga tgctattgct
    52801 ataatgatgg cggcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
    52861 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    52921 aatgttccgg tttatgcggg gatgccgcag cccattatgc gtcaacaaat cgttgccgat
    52981 aatattcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    53041 gaaagcactc atgcggtgaa atatatcatt gataccctga tggcaagcga tggcgatatc
    53101 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgcg
    53161 atcctgccca aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc
    53221 acgccatctg ccgagttcaa catctttgcc gacccggaag ccgcacgcgt agtgttcacc
    53281 tccggcgttc cattagtgat gatgggcctc gatctcacca accagaccgt ttgcaccccg
    53341 gacgtgattg ctcggatgga aagggcaggc ggccccgccg gagagctgtt cagcgacatc
    53401 atgaacttca ctctcaaaac gcagttcgaa aactacggcc ttgctggcgg cccggtgcac
    53461 gacgccacct gcatcggtta tctgattaac cctgatggca ttaaaaccca ggagatgtac
    53521 gtcgaagtgg acgtcaacag tggcccttgc tatgggcgta ccgtctgcga cgagctgggc
    53581 gttcttggca agcccgccaa taccaaagtc ggcatcacta ttgatacaga ctggttctgg
    53641 ggattagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    53701 cgggccggta tcgcaagatg caggcctggc tatttctgat gggcagaaag ccgtcgccca
    53761 atatgacatg aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta
    53821 ttgctgacga ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt
    53881 ggcgcggcgt tagtgttaca ggtcgtgatt ggcggcatta tgctttggtt accgccaggg
    53941 cgttgggtcg ctgaaaaagt cgcttttggc gtgcataaag tgatggcgta cagcgacgcg
    54001 ggtagcgcat ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggt
    54061 gcaggattta tctttggttt cagggtgtta ccggcaatta tcttcgtcac cgcgctggtg
    54121 agtattctct actacatcgg tgtgatgggg attttaattc gaattctcgg cggtatcttc
    54181 cagaaagcat taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc
    54241 gggcaaaacg aaattccggc aatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa
    54301 ttatttacag cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac
    54361 gccgcactgg gcgtgcctgt ggaatatctg ctggcggcat cattaatggc gatccctggc
    54421 gggatcttgt ttgcccgcct gttaagcccg gcaacggaat cttcgcaggt ttcctttaat
    54481 aacctctctt tcaccgaaac accgccaaaa agcattattg aagccgctgc gacaggggca
    54541 atgaccgggc tgaaaatcgc cgcaggtgtg gcaacagtgg tgatggcatt tgttgcaata
    54601 attgcgttga ttaacggtat tatcggcggc gttggtggct ggtttggttt tgaacatgcc
    54661 tcgctggagt ccattttagg ttacctgctg gctccactgg cgtgggtgat gggtgtggac
    54721 tggagtgatg cgaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt
    54781 gtcgcttatc tcaatttctc accctatctg caaacggctg gcactctcga tgctaaaact
    54841 gtggcgatta tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    54901 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta
    54961 cgggcgctgg cggcggcgac gctttccaac ttgatgagtg cgaccattgc cgggttcttt
    55021 attggtttag cttgagtgtc attgcctgat gcgca
//
LOCUS       DAHBHC010000025        53891 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHBHC010000025 DAHBHC010000000
VERSION     DAHBHC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.025"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..768)
                     /locus_tag="M2A35_001890"
     CDS             complement(<1..768)
                     /locus_tag="M2A35_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCB7198199.1"
                     /translation="MSKIDYQVLREAAEKATQGEWVAFISSDTGTYAVHTPGDKRCED
                     VIKWTGFDGQKNAENNARYIAAFNPEVVQALLDERERNQQYIKSRDQENEEIALTVGK
                     LRVELEAAKKRIAELEAEPVSQTYNLPELIEGMEVSIDVSTCDADLGNRYFGTVTEAL
                     ELDTAKNGYILLVQDAEPNFDVNGNSPGTPDSWISCSDRMPEKNQNVLISVNFDSSLV
                     EPLICSARYTGSTFRRGDATIKPGNGIEQATHWMPLPE"
     gene            complement(770..1069)
                     /locus_tag="M2A35_001891"
     CDS             complement(770..1069)
                     /locus_tag="M2A35_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198200.1"
                     /translation="MSVYLIDKRRRGQQIPPVGIPNHTWFCVLDIDGMDSLVDTRHYC
                     DTATATPAKAKKMAALIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            complement(1066..1560)
                     /locus_tag="M2A35_001892"
     CDS             complement(1066..1560)
                     /locus_tag="M2A35_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198201.1"
                     /translation="MESNIKGLVSAGHEMASELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANALAEDHQKAIESIKQADAAVKLAHEKFAALAAENAGLKKFCKDAAFDADYEA
                     ELGMERGGFSDALNEIKTPATDAFLAEVRAQGVEMFAECAYTLEHHDHAVAFAAELRK
                     GGNQ"
     gene            complement(1622..1966)
                     /locus_tag="M2A35_001893"
     CDS             complement(1622..1966)
                     /locus_tag="M2A35_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCB7198202.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLELRKRAGEIAWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGFWTDDARVKTKVAADQYPFRIIGVTVKP
                     KKAGGGWNIEEF"
     gene            complement(2084..2869)
                     /locus_tag="M2A35_001894"
     CDS             complement(2084..2869)
                     /locus_tag="M2A35_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7198203.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYTNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKPEPMSTPPDTG
                     RDVYHPPGRSFGCMPNAATLGGITPAQWLMEEYRRGKASGFIK"
     gene            complement(2866..3681)
                     /locus_tag="M2A35_001895"
     CDS             complement(2866..3681)
                     /locus_tag="M2A35_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7198204.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLV
                     KDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRVRVFSLNITKNGNIKGNREGGNE
                     PENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPH
                     DPGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAE
                     FRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWNSPEAWEDFL"
     gene            complement(4048..4278)
                     /locus_tag="M2A35_001896"
     CDS             complement(4048..4278)
                     /locus_tag="M2A35_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198205.1"
                     /translation="MSDNEHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            4433..5017
                     /locus_tag="M2A35_001897"
     CDS             4433..5017
                     /locus_tag="M2A35_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7198206.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVISQNERSKQ"
     gene            5326..5625
                     /locus_tag="M2A35_001898"
     CDS             5326..5625
                     /locus_tag="M2A35_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198207.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLSS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDSYRREAMAIIARWDLADQLDGQREAA"
     gene            5622..6443
                     /locus_tag="M2A35_001899"
     CDS             5622..6443
                     /locus_tag="M2A35_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HCB7198208.1"
                     /translation="MKPGIYFDISNEDYHAGDGVSKSQLDMVAKNPALLKWVQAAPED
                     EEKKSALDMGTALHCLLLEPGEFDKRFIVSPKFDRRTKQGKADEEAFLRDVADMGITV
                     LDAEQWRKLELMRDSAMAHPAARWMLEAPGYCEASMYWNDEETGELCRIRPDKWLNEH
                     NVIVDVKKVADMDRFARHIEEFRYHVQDAMYREGAMRITGQPHGFFFLAVSESIDCGR
                     YPVRVFGLDAPDVDAGHALFRRDLNTYHECRISDEWGGVEIIKRPEWARKQDMYV"
     gene            6440..7357
                     /gene="bet"
                     /locus_tag="M2A35_001900"
     CDS             6440..7357
                     /gene="bet"
                     /locus_tag="M2A35_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HCB7198209.1"
                     /translation="MSNEIAITNDVLAIRGIDEVTWSALKNSIYPGAKDESVMMAVDY
                     CRARQLDPLLKPVHLVPMSVKDSKSGKNEWRDVVMPGIGLYRIQAYRSGDYAGANEPE
                     FGPDVTQTLSGVEVTFPQWCKYTVSKRMASGEIVEFSAKEYWIENYATGGRDTSAPNA
                     MWKKRPYAQLAKCAEAQALRKAWPEIGQQATAEEMEGKYIDSPDIIERDVTPRSQAKH
                     GTASSMNSLINAKTVKKPDEQTRKADSRDPEEMLMAFTSAAMNYSTVSELDKAYKYIA
                     QKLSDDDELLAKATDVYSVRREELNETSM"
     gene            7407..7655
                     /locus_tag="M2A35_001901"
     CDS             7407..7655
                     /locus_tag="M2A35_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCB7198210.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            7813..8064
                     /locus_tag="M2A35_001902"
     CDS             7813..8064
                     /locus_tag="M2A35_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7198211.1"
                     /translation="MRSPSKIVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYE"
     gene            8093..9160
                     /locus_tag="M2A35_001903"
     CDS             8093..9160
                     /locus_tag="M2A35_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HCB7198212.1"
                     /translation="MSKVGDYFFEFPASRGTQGSTVILMMTVPARALSRVLATDNTGS
                     TLERSQREINPTRVKKFYQYLVNAYEKKEPFIVPPLVGNCNSEIEFHEIGNTNVGLAR
                     FPMDAEIKLFDGQHRAAGLAEFCRNYGETIMIPLMLTHNLPLKARQQFFSDINNNVSK
                     PSAAINMAYDGRNDVAQEMVSFLSQHDTFAEVTDFEHNVVPSKSNMWVSFKALSDSTS
                     KFCIHNGKKLEIGNIESVWESWLCLTQLEAIRHGTKQSEYKREYIQFHAVMINAFGYA
                     VQQMMVNHSIKEIISMIETLCANTDFAERENFFLMDNWVGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLGAEQ"
     gene            9157..9804
                     /locus_tag="M2A35_001904"
     CDS             9157..9804
                     /locus_tag="M2A35_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methylase"
                     /protein_id="HCB7198213.1"
                     /translation="MKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVW
                     DLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGHM
                     TRANSEDCLFAVKGKLPPRINAGIVQSFTAPRLEHSRKPDVVREKLVQLLGDVSRIEL
                     FARQTSHGFDVWGNQCEDPAVQLHPGYALYIAGLTNAFSNAPLSPTDNQGRERAA"
     gene            9801..9995
                     /locus_tag="M2A35_001905"
     CDS             9801..9995
                     /locus_tag="M2A35_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HCB7198214.1"
                     /translation="MNRASPADLRKCLETANMLAHSGIRFVPIPAVTDAEFATLSAIF
                     TDKIESLAAEAEMEENQQNN"
     gene            complement(9999..11249)
                     /locus_tag="M2A35_001906"
     CDS             complement(9999..11249)
                     /locus_tag="M2A35_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7198215.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMAEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQGFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WIDGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(11442..13019)
                     /gene="guaA"
                     /locus_tag="M2A35_001907"
     CDS             complement(11442..13019)
                     /gene="guaA"
                     /locus_tag="M2A35_001907"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCB7198216.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDAKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(13088..14554)
                     /gene="guaB"
                     /locus_tag="M2A35_001908"
     CDS             complement(13088..14554)
                     /gene="guaB"
                     /locus_tag="M2A35_001908"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCB7198217.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14716..16086
                     /gene="xseA"
                     /locus_tag="M2A35_001909"
     CDS             14716..16086
                     /gene="xseA"
                     /locus_tag="M2A35_001909"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCB7198218.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(16083..16298)
                     /locus_tag="M2A35_001910"
     CDS             complement(16083..16298)
                     /locus_tag="M2A35_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCB7198219.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(16367..17839)
                     /gene="der"
                     /locus_tag="M2A35_001911"
     CDS             complement(16367..17839)
                     /gene="der"
                     /locus_tag="M2A35_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCB7198220.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEYAEYWEQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(17957..19135)
                     /gene="bamB"
                     /locus_tag="M2A35_001912"
     CDS             complement(17957..19135)
                     /gene="bamB"
                     /locus_tag="M2A35_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCB7198221.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(19146..19766)
                     /gene="yfgM"
                     /locus_tag="M2A35_001913"
     CDS             complement(19146..19766)
                     /gene="yfgM"
                     /locus_tag="M2A35_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCB7198222.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(19784..21058)
                     /gene="hisS"
                     /locus_tag="M2A35_001914"
     CDS             complement(19784..21058)
                     /gene="hisS"
                     /locus_tag="M2A35_001914"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCB7198223.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(21169..22287)
                     /gene="ispG"
                     /locus_tag="M2A35_001915"
                     /gene_synonym="gcpE"
     CDS             complement(21169..22287)
                     /gene="ispG"
                     /locus_tag="M2A35_001915"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCB7198224.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(22314..23327)
                     /gene="rodZ"
                     /locus_tag="M2A35_001916"
     CDS             complement(22314..23327)
                     /gene="rodZ"
                     /locus_tag="M2A35_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCB7198225.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(23612..24766)
                     /gene="trmG/rlmN"
                     /locus_tag="M2A35_001917"
     CDS             complement(23612..24766)
                     /gene="trmG/rlmN"
                     /locus_tag="M2A35_001917"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCB7198226.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(24916..25347)
                     /gene="ndk"
                     /locus_tag="M2A35_001918"
     CDS             complement(24916..25347)
                     /gene="ndk"
                     /locus_tag="M2A35_001918"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCB7198227.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(25496..27808)
                     /gene="pbpC"
                     /locus_tag="M2A35_001919"
     CDS             complement(25496..27808)
                     /gene="pbpC"
                     /locus_tag="M2A35_001919"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCB7198228.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWPAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPDQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(27809..32770)
                     /gene="yfhM"
                     /locus_tag="M2A35_001920"
     CDS             complement(27809..32770)
                     /gene="yfhM"
                     /locus_tag="M2A35_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCB7198229.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            32977..33822
                     /gene="sseA"
                     /locus_tag="M2A35_001921"
     CDS             32977..33822
                     /gene="sseA"
                     /locus_tag="M2A35_001921"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCB7198230.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(34481..35257)
                     /gene="sseB"
                     /locus_tag="M2A35_001922"
     CDS             complement(34481..35257)
                     /gene="sseB"
                     /locus_tag="M2A35_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCB7198231.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(35399..36682)
                     /gene="pepB"
                     /locus_tag="M2A35_001923"
     CDS             complement(35399..36682)
                     /gene="pepB"
                     /locus_tag="M2A35_001923"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCB7198232.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(36860..37060)
                     /gene="iscX"
                     /locus_tag="M2A35_001924"
     CDS             complement(36860..37060)
                     /gene="iscX"
                     /locus_tag="M2A35_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCB7198233.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(37072..37407)
                     /gene="fdx"
                     /locus_tag="M2A35_001925"
     CDS             complement(37072..37407)
                     /gene="fdx"
                     /locus_tag="M2A35_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCB7198234.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(37409..39259)
                     /gene="hscA"
                     /locus_tag="M2A35_001926"
     CDS             complement(37409..39259)
                     /gene="hscA"
                     /locus_tag="M2A35_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCB7198235.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(39276..39791)
                     /gene="hscB"
                     /locus_tag="M2A35_001927"
     CDS             complement(39276..39791)
                     /gene="hscB"
                     /locus_tag="M2A35_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCB7198236.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(39887..40210)
                     /gene="iscA"
                     /locus_tag="M2A35_001928"
     CDS             complement(39887..40210)
                     /gene="iscA"
                     /locus_tag="M2A35_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCB7198237.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(40227..40613)
                     /gene="iscU"
                     /locus_tag="M2A35_001929"
     CDS             complement(40227..40613)
                     /gene="iscU"
                     /locus_tag="M2A35_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCB7198238.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(40641..41855)
                     /gene="iscS"
                     /locus_tag="M2A35_001930"
     CDS             complement(40641..41855)
                     /gene="iscS"
                     /locus_tag="M2A35_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCB7198239.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(41967..42455)
                     /gene="iscR"
                     /locus_tag="M2A35_001931"
     CDS             complement(41967..42455)
                     /gene="iscR"
                     /locus_tag="M2A35_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCB7198240.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(42725..43465)
                     /gene="trmJ"
                     /locus_tag="M2A35_001932"
     CDS             complement(42725..43465)
                     /gene="trmJ"
                     /locus_tag="M2A35_001932"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCB7198241.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            43584..44387
                     /gene="suhB"
                     /locus_tag="M2A35_001933"
     CDS             43584..44387
                     /gene="suhB"
                     /locus_tag="M2A35_001933"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCB7198242.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            44532..45386
                     /locus_tag="M2A35_001934"
     CDS             44532..45386
                     /locus_tag="M2A35_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7198243.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            45577..46857
                     /gene="csiE"
                     /locus_tag="M2A35_001935"
     CDS             45577..46857
                     /gene="csiE"
                     /locus_tag="M2A35_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCB7198244.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRRWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNYFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(46849..47988)
                     /locus_tag="M2A35_001936"
     CDS             complement(46849..47988)
                     /locus_tag="M2A35_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCB7198245.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(48148..49038)
                     /gene="hcaR"
                     /locus_tag="M2A35_001937"
     CDS             complement(48148..49038)
                     /gene="hcaR"
                     /locus_tag="M2A35_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCB7198246.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            49174..50535
                     /locus_tag="M2A35_001938"
     CDS             49174..50535
                     /locus_tag="M2A35_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCB7198247.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFDPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            50532..51050
                     /locus_tag="M2A35_001939"
     CDS             50532..51050
                     /locus_tag="M2A35_001939"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCB7198248.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            51050..51370
                     /locus_tag="M2A35_001940"
     CDS             51050..51370
                     /locus_tag="M2A35_001940"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCB7198249.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            51367..52179
                     /gene="hcaB"
                     /locus_tag="M2A35_001941"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             51367..52179
                     /gene="hcaB"
                     /locus_tag="M2A35_001941"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCB7198250.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            52189..53391
                     /locus_tag="M2A35_001942"
     CDS             52189..53391
                     /locus_tag="M2A35_001942"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCB7198251.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            53488..>53891
                     /locus_tag="M2A35_001943"
     CDS             53488..>53891
                     /locus_tag="M2A35_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCB7198252.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGA"
BASE COUNT        12948 a        14495 c        13374 g        13074 t
ORIGIN      
        1 ttccggtagc ggcatccagt gagttgcttg ctcaatacca ttacccggct taatcgttgc
       61 atctccgcgc cgaaaggtgc ttccggtata gcgtgcggag catattagcg gttcaaccag
      121 agagctatcg aaattcaccg aaataagcac gttctgattc ttttccggca ttcgatcact
      181 acagcttatc caactatccg gagttcccgg agagttgcca tttacatcga agtttggctc
      241 tgcgtcctga accaggagga tgtaaccatt cttggcagta tcaagttcta acgcctcggt
      301 gacggtgccg aaatagcgat tacctaaatc agcatcacaa gtgcttacat caatggaaac
      361 ttccatgccc tcgattaatt ctggcaagtt gtaagtttgg cttacgggtt cggcttccag
      421 ttctgctatg cgcttttttg ctgcttccag ctcaacacgc agcttcccta ccgttagcgc
      481 aatttcctcg ttctcctggt cgcgtgattt gatgtattgc tggtttcttt cccgttcatc
      541 cagcagcgcc tgcactactt cagggttgaa agctgcgata taacgagcgt tgttctctgc
      601 atttttctgt ccatcaaagc cggtccattt gataacgtct tcacatcgtt tatcaccggg
      661 tgtatgcacc gcatatgtac cagtatccga cgaaataaat gcaacccatt cgccctgtgt
      721 tgccttttct gccgcctcac gcagtacctg atagtcaatc ttgctcatgt cacatcaccc
      781 tgaatccgtt gcatttacgt aaaaaatcgc agatatagcc cttcattttt tcgtgccaat
      841 ctcgatcatt cccattgcac caaccatcag gtggagtcca gttttctatc agagcagcca
      901 ttttctttgc ttttgcagga gtagctgttg cggtatcgca gtaatgacga gtgtcaacca
      961 acgaatccat accatcgata tcaagtacgc aaaaccatgt gtgattcgga attcctacag
     1021 gtggtatttg ttgcccacgt cgacgtttat caataagata tacactcact ggttgcctcc
     1081 tttgcgaagc tcagcggcga aggctactgc gtgatcatga tgttcaagtg tgtatgcaca
     1141 ctccgcaaac atctccacgc cctgcgcccg tacttcagcc agaaaagcat cggtggctgg
     1201 ggttttgatt tcgttaagcg catcactgaa tccaccacgc tccataccta gctctgcttc
     1261 gtaatcggca tcgaatgcag cgtctttgca gaacttcttc agccccgcat tctccgccgc
     1321 cagcgccgca aacttctcgt gtgccaactt aacagccgca tcagcctgct taattgactc
     1381 aatcgctttc tggtggtctt cggccagcgc attagcacgc accagttgca cttccagttg
     1441 cgttgccaaa tcgctgatca gctttgccac actgcgcata tcaacggcac cacattctgc
     1501 tttcagttcc gaagccatct catgcccggc ggaaactaac cctttgatat tactttccat
     1561 ctttaccctc gcttatccac ataacttatt gattacattg ataactaaaa agatcgtcga
     1621 ttcagaactc ttcgatgttc cagccaccac ctgctttctt tggcttaacc gttaccccga
     1681 tgattcggaa cggatactga tctgcggcga ctttggtttt caccctggcg tcgtcggtcc
     1741 agaaaccttt cacttcgtgc agttccatct ctccggtggc gagcatcaca gcgaaatctg
     1801 gcgtatagaa cgtgttgtca gctaaccgca gcttgatacc ctcgaatcga taccaggcga
     1861 tttcccctgc acgtttacgc agctcaaggt gctggcaata cgcagattct gttttgttca
     1921 tctggcctgt tttgagtcga ccaagagcct gtatctgttt tctcatgatt tacctctgag
     1981 gtaattaaaa accacataag acacgaaatc aatagatgtt agaacatttt attacctaac
     2041 aggtaattat cgagacataa aaaaatgcgc tatcgcgctg gtattacttg ataaatcctg
     2101 acgcctttcc ccgcctgtat tcctccatca gccactgcgc cggtgttatt ccccccaggg
     2161 tggcggcgtt aggcatgcac ccgaaacttc gccctggtgg atggtaaacg tctctccctg
     2221 tgtccggggg tgtactcatg ggctctggct ttgcctgtat gctgatcacc ggatcgggta
     2281 tctgctgtcc ggaagccacc tttttcgccc aatcatcgag cagcctgcgc gcgtgtttct
     2341 caacctcaat ctcgctaagc tggcgctgat acattgcacg gcgtgtatca catacgaccc
     2401 agtacataac cggatgccgc cacgggaatc tttcgggacc accaggatat aaactttttt
     2461 ccttgctgta ccggtgaaac tccgccatca catcgtcaat ggtgacgcca agaaccatct
     2521 tgctgtcttt acaccacttg atgaattgcc ctggcgacgg ccagaacgga gattcactgg
     2581 cgcgggcgtg gcgcatacca gcagaaacct gttcacgggt tcggatcccc ccttcggcaa
     2641 acgcagcaat ccactgctgt tttgcagcaa cttcctgctc tggcgtcttc aggttggtta
     2701 ccactgccgc cggaaacagt tgtttcagct gtttgaaaag ggcatcaaca agcctctctg
     2761 ctgacatgtt caccacgttg tcattgttgg tgtactgatg ctcataacct gacatgcgag
     2821 aaagggcttc tccgtcacgg ttttgtatcg cggtaaaaac gttgttcaca agaaatcctc
     2881 ccatgcttca gggctgttcc agtgcggaac gttgttatca ggtaatgttg attgcttctg
     2941 tctgctaatc tgcagccgcc ttgccagctt ctgctcccac tgtgcctgat ggtatgcctt
     3001 accctcagcc atccagtaaa ttctgaactc tgcaagttcc tgtgccgttg gcagactgtc
     3061 caggtagatc ccctgcaatg agcttttccg aagaaagtca tctgatggct gccattgttc
     3121 atgcatgaca aatttgccta attgccctgg cccaccagga ggaacaaagt tattcatcac
     3181 ggcgttgttt gcgccggggt catgaggaac agaatccccg gtttttgtcc tgctctccct
     3241 ctcttggtta aatgactggt tatatgactg gttctggatc ccgtttttgg gatcattcaa
     3301 catcccgttt ttgggatcat tcaacatccc gtttttgggt atattcccgt tttcggtaac
     3361 attaccgttt tcgggttcat tgcccccctc ccggttgcct ttaatgttcc cgtttttggt
     3421 tatattaaga gagaaaaccc gcactctttt cgtcgctccc tttctctctc cggtatctga
     3481 aataagcccc attttcatga gcgatataag cccggcctgc acggtttttt tattcaggca
     3541 agtgtcttta acgaggcgtt ctatgctggg gtagcagagg ttatattcat cggctctgtc
     3601 agccatcgag agcagtatga gctttaatga cgagctacct ggatctgtct cccaggccca
     3661 atctgttgca tgtctgctca tgattaatct ccgctatcag cttgaatgtt gtggggagga
     3721 attaatcatg atctgcttaa tctctgccct gatatgacgg tttgattcca tggtgcactc
     3781 aacacagtgt ccgttgtaaa cccagcgttc actgtcatgt ccgtgcttac atgtttttcc
     3841 ggtgtagtag cgtttaagtc cgcgctttgc ggcatcaata cgtgtaatga tttccatggt
     3901 aagccctgct attagtattg ggattacggt cattttgtgc tgacacaaaa aaaagatcaa
     3961 ccatatttgg ttttttatta cctttgaggt acgaatagat atgaaaagac cgccggatgg
     4021 cggtctacag agggttgtag ctggatatca tgagtagaag aagtatgcca gttctgcttt
     4081 tgagcgcagc cattgtcttg ttttacaggc tttaaaaagc ccattcatca ataccttacc
     4141 tggcattttg cgcttacctg ttaagtgagt ctggatatag tgactcgtcg ttccggcttc
     4201 ctgtgcgaag gcttcacgct catccggagt aagtgcaagc cagtgctttt tgaaatcgaa
     4261 atgttcgtta tcgctcatag ctattgcctg atatttattt cagataataa atattcaccc
     4321 ataaggtaac aaaaatcaag gatagttacc tatggggtgc atttacctgt tgggtaatat
     4381 tgctttaaat tgaatcatct actgattcat atatgaggcg attttccaga aaatgaaaag
     4441 tatccaagac gtccgcaggc aaaatctcaa cgacttgatc gaccgtgaat tcaatggtgt
     4501 tcagacgcgg atggcagaaa aacttggaac tcaggcaaat ctggtaaacc gctgggctct
     4561 tggcaagaag gttatcggcg accaggttgc gcgaaaaatt gaagctgccg ccaataaacc
     4621 acgtaactgg cttgatatcg atcgctcgct ttctcaggaa ggttttcagc ctgtcggccc
     4681 aagcgacatt ggtcagctgg cggctcacaa cctggaacgc tggatgagcg aaagccgcga
     4741 cctttcaacg cagggaaaac tacaccgcgc atccggcgtc gcccaggtga caatcagccg
     4801 cctgttaaac aatgaagtca gcgtttccat ttccaccctg gagaatgttg catccgcatt
     4861 cgggcgtcac ggctatgaat tactgattca cccgcacgac cctgcgacta tcaactatga
     4921 ccgctcgcgc tacgcattgt tacccgaaac agagaaggca aagatcgaaa gttacattga
     4981 atttgtcatc agccagaacg aaagaagcaa acaataaaac tatagttttc agtaagtaag
     5041 ccgcctcatg gcggcttttt tattgccaac aagattacct tatgggtaat ttttttaact
     5101 catacctatt gacatcaaac caaatacgca taattattac ctcaacggta acagaccgag
     5161 gtaacaagtt atgcagtgga aaatcatcaa cggttggtac tgcgttactg catgcggatt
     5221 catgagctgg aagttccgca ccttacagga aggcattaag tgggctttcg tcagcaaaga
     5281 agctcgcgat gtggccaacg ataacgagat atgggagggc tgataatgaa cgttaatcag
     5341 cagaaaaatc ttcaaaaaat catgctggca ttcgacaagg actaccgcct gtcagaacag
     5401 ctatatgacc gacaagttga actgattgag agcatccgac ttcatcaact gtcctcaact
     5461 ttcgacgttg taacaggcaa aggcgttcgt caggaagtgc tggaggctgc taaagacagc
     5521 cctgagttcg aagaactgat ggattcctac cggcgcgagg caatggcaat tatagcgcgc
     5581 tgggatctgg cggatcagct tgatgggcag agggaagcgg catgaaaccg ggaatttatt
     5641 tcgacatcag caacgaagac taccacgccg gtgacggcgt gagtaagtcg caactggaca
     5701 tggttgccaa gaatccggcg cttcttaaat gggttcaggc agcaccagaa gacgaagaga
     5761 aaaagtctgc actggatatg ggaaccgcat tgcactgtct gcttctggag cctggagaat
     5821 tcgacaaacg cttcattgtt tcaccgaagt tcgatcgtcg gacgaaacaa ggtaaagctg
     5881 acgaagaagc atttcttcgt gatgtagcgg atatggggat tacggtactt gatgccgagc
     5941 agtggcggaa actggagctg atgcgtgata gcgcaatggc tcacccggcg gcacgctgga
     6001 tgctggaagc acctggttac tgcgaagcat caatgtactg gaacgatgaa gagacgggtg
     6061 agttgtgccg aattcgtcca gacaaatggc tgaacgagca caacgtgatc gtcgacgtga
     6121 aaaaggttgc agatatggac cgttttgcac gtcacatcga ggaattccgc taccacgtgc
     6181 aggacgcaat gtaccgcgaa ggcgcaatga ggattactgg tcagccgcat ggttttttct
     6241 ttcttgccgt gagcgaaagc attgattgtg gtcggtatcc ggtacgcgtg ttcgggctgg
     6301 atgcgccgga tgtcgatgcc gggcacgctc tgttccgccg ggatctgaat acctatcacg
     6361 aatgccgcat cagcgatgaa tggggcggcg tggaaattat taaacgccct gagtgggcac
     6421 gcaaacagga tatgtacgta tgagcaacga aattgcaatc accaatgatg tgttggctat
     6481 tcggggaatc gatgaagtta catggagcgc tctgaaaaac agtatttacc ctggggcaaa
     6541 agatgaatcg gtaatgatgg cagtcgatta ctgccgggcg cgtcaactcg atcctcttct
     6601 taagccggta cacctggtac caatgagtgt taaagactcg aagagtggaa agaatgaatg
     6661 gcgcgacgtg gtcatgcctg gcattggact ttatcgcatt caggcctacc gttccggcga
     6721 ttacgcaggc gctaatgaac ctgagttcgg tccagatgta acacaaacgc tttcaggcgt
     6781 ggaggttacc ttccctcagt ggtgtaagta caccgtcagc aagcgaatgg caagcgggga
     6841 aatcgtagag tttagcgcca aagaatactg gattgagaac tatgcaactg gggggcgtga
     6901 cacatcggca ccaaatgcca tgtggaaaaa gcgcccttat gcgcagttgg caaaatgcgc
     6961 agaggcacag gcattacgta aagcatggcc ggagattggt caacaagcca cagctgagga
     7021 aatggaaggt aaatatatcg actcacctga cattattgaa cgtgacgtaa ctccaagaag
     7081 tcaggcaaag cacggcacag catccagcat gaacagtctg atcaacgcta aaacagtgaa
     7141 aaagcctgat gagcaaacgc gtaaagcgga tagccgtgat ccagaagaaa tgctgatggc
     7201 ctttaccagc gcagcgatga attacagcac tgtctccgaa ctggataagg cttacaaata
     7261 cattgcacaa aaactttcag atgatgacga actgctggca aaagccaccg acgtttacag
     7321 cgttcgtcgg gaagaattaa acgaaacatc tatgtaacca ccaccgcggc gccacgcgcg
     7381 ccgcactgca accaagagag gtatttatga aaggtgcatt aggtaagaag gaactcctgg
     7441 cggtggtgcc actgtcatgg agcactatcg accgtatgga gcgcgcaggt gaatttccta
     7501 aacgctggta tatcactgac aaacgctgcg catggaaccg tgacgaagtt gagcgttggc
     7561 ttgatgaacg tcaggcagca agcccggcag agttccaggg taaaaagcct cctgttcagc
     7621 aacgtgtata tcgtcctgtg agcaacgctg catgagtgtg ctgctaaggc actggagcaa
     7681 atggtcagga tggtacttat tcctggcctc tgtttcagca tggctttatc tgctggcatt
     7741 aattttcaga gagggttgga ttaagtgaga aagttaagcc gacttgaaaa atatcacatg
     7801 aataaggttt caatgcgcag tccgtcaaag attgtcgccg ttactcctgc ggcgatagag
     7861 atcgaaaaac gcgcgattga aagagagaaa aaagggcaat tccgcattgc cgctcacctt
     7921 tggcttcagt gtatggatgt tgcttctggt gatgttgaac gtgcaaggat cgcggttcgc
     7981 agggaccaat gtatcacaaa aggtaacggc cttcgccgtg gcgactatag cggcatagga
     8041 tgttgcgggg tggtttatga ataatcaaaa caataatgta aaaggtaata tcatgagcaa
     8101 ggtaggcgat tatttctttg aatttcccgc atcacgagga acgcagggaa gcactgtaat
     8161 acttatgatg acagtaccag caagagcact atcgagagta ttggcaaccg acaatacggg
     8221 aagtaccctt gaacgttctc aacgtgaaat taatccaacc agggtaaaaa agttttatca
     8281 atatttagtc aacgcttacg agaaaaagga accatttatt gttccaccat tggtcggaaa
     8341 ttgtaactca gaaatagagt tccatgagat tggaaatact aatgtcggtt tagctagatt
     8401 tcctatggat gcagaaataa aattatttga tggacagcat cgtgctgcag gattagctga
     8461 gttctgccgt aattatggcg aaacaattat gattcctctt atgcttacgc ataaccttcc
     8521 tttgaaggcg cgtcagcagt tcttttctga cattaataat aatgtttcaa aaccatctgc
     8581 agcaattaat atggcgtatg acggacgaaa cgatgttgcc caagaaatgg tgtctttcct
     8641 gtcgcaacat gacacttttg cagaagtcac tgacttcgag cacaacgttg ttccgtcaaa
     8701 gtcaaacatg tgggtgagtt tcaaagcatt aagtgattca acatcaaaat tttgtattca
     8761 taatgggaaa aaactagaaa ttggtaacat cgagtcagta tgggaatcat ggttatgtct
     8821 tacacaacta gaagcaattc gccatggtac aaagcaatca gaatacaaaa gagaatatat
     8881 ccagtttcat gcagtgatga ttaacgcttt tggctatgct gttcagcaaa tgatggttaa
     8941 tcactcaata aaagaaataa tttcaatgat tgaaacatta tgtgcaaata ctgacttcgc
     9001 agaaagagag aattttttcc taatggataa ttgggttggg atctgcacga aagcaagcca
     9061 ggaaaaacta tcagttattg ccaatgtagc agcacagaaa gcggcagcaa acaggctgat
     9121 acaagccttt accaaaggaa gtctgggagc agaacaatga aaaaatacac gctaatctat
     9181 gcagatccac cctgggtata ccgggacaaa gccgcagatg gtaatcgcgg tgccggtttt
     9241 aaatatccgg ttatgagtgt gctggatatc tgccgccttc ctgtgtggga tttggccgat
     9301 gaaaactgtc tgttggccat gtggtgggtg ccaacacaac cactcgaagc actaaaagtt
     9361 gttgaagcct ggggatttcg tctgatgacc atgaagggct tcacgtggat aaaatgtggt
     9421 agtcgacaac cagataaact ggttatgggg atgggacaca tgactcgcgc caacagtgaa
     9481 gattgcctgt ttgcggtaaa gggaaaacta cctccgcgca ttaatgcagg tatcgttcag
     9541 tcatttaccg caccgcggct tgagcattca agaaaaccag atgtcgttcg tgaaaaactt
     9601 gtgcaattgt taggcgatgt ttctcgcatt gaactgttcg cccgccaaac gtctcatggc
     9661 ttcgatgttt ggggtaatca gtgcgaagac ccagcagtgc aactacaccc tggatacgcg
     9721 ttgtatattg ccggattaac aaatgcattc agcaatgctc cgctgtcacc aacagacaac
     9781 caggggcggg agcgtgcagc atgaacaggg catcaccagc agatttaagg aaatgccttg
     9841 aaactgcaaa catgcttgca cacagcggga tcaggtttgt tccaattccc gctgtcactg
     9901 atgctgaatt tgcaacactg tcagcaatat tcacagataa aattgaatca ctggcagcag
     9961 aagccgagat ggaagaaaat cagcagaata attaaacgtt attcccccgc catccacttc
    10021 tcaaacttcg acggggagaa cggaatcaga tccgtatgct ccccgtcaat ccaggaatca
    10081 atcatatcgg cccactgctg caacatgtag gcgcgctgtc tggcgtattc cgctttgtta
    10141 tatacggcgc gcacaccttt ctgctcatgt gccagagcct tttcaatcca gtctgaagga
    10201 taaccagcct catgcaacaa cgtactggct gtacggcgca tatcgtgtac ggtgaagccc
    10261 tgaatatgct caccatcttc atttattatt ttcaccgttc tgtcgatcag agagttcagc
    10321 gcggcattag ataatggctt ccggaaattg taacgaccag gaaccagata ttcacttcca
    10381 ccagcgcaca tctgcaaccc gaccaatata tcctgagcct gtttaggcag gtaaataacg
    10441 tgcgcccggc ttcccttcat gcggtctgaa ggaattgtcc atgtccattt tttaaaatct
    10501 atttcatccc acgttgcgtt ggtgaattcg cctttacgaa ccatagtgat aagcaccagc
    10561 tttaaagcca ttttcatagt gcccatagca ccaatggcat ccagcgtgcg gaagaacagg
    10621 ccaatttctt ctggtgtcag tgttcgctct cttggtttaa atatggcgat agacgaaggt
    10681 ttaatgtcag ccgcaggatt aaacaaacca tgaccacggt cattggcgtg acggtatacg
    10741 ctactgatga tctccctggc ctgcactgct gttgcccggc caccgcgttc gacaatccgg
    10801 tcacacaaat cacgaaccat cgatgtggta atttcagcca tcattttatt gccaagaacc
    10861 ggaagtatgt cacggtcgat caccgcctgt ttcattgcgc gggtactgtc agccaggatg
    10921 acgtgtttca tataactgtc ggtatgtacc gcaaacgtct cggcaccacg aatctttttg
    10981 ataccgtcac gtttagccgc agccggtgac tggcctgctt taagcagctt ctttgcagca
    11041 atcagttctt ctcgcgcttc tgccaggctg ataccgtcac gcccatactg cccgattacc
    11101 agcgtttcgc ggcgaccgtt gatacggtag tcgtagcgaa acgagaccgt gcctgacgta
    11161 agcacagcta catacagccc gtcacgatcg gagaccttgt acagtttgtc ctgcggcttg
    11221 aggtttttta attttgtatc ggtaagcaca attcacccgt atagaaacca ttttcatgac
    11281 ggtatgagag tataccttta aggtaatacc gtcacctgta ccgccgaaaa atatggtgta
    11341 gagtgaatag aaatgaatac ataaaaacaa aaaccctctg taaaaacaga gggttgagtt
    11401 agtgtctgaa tagaaatgat ttgctatgag ttagctatta atcattccca ctcaatggta
    11461 gctggcggct tgccgctgat gtcatacacc acgcgggaaa taccgttcac ttcattgata
    11521 atgcggttgg aaacgcgacc gaggaaatcg tacggcagat gcgcccagtg tgcggtcata
    11581 aagtcgatgg tttcgacagc acgcagagag acaacccagt catacttacg accatcgccc
    11641 attacgccaa cggaacgtac cggcaggaac acagtgaacg cctggctgac tttgtcgtac
    11701 aggtccgctt tacgcagttc ttcaatgaag atggcgtcag cacggcgcag caggtcacag
    11761 tactctttct tcacttcacc cagcacacga acgccaaggc ctggtcccgg gaacgggtga
    11821 cggtacagca tgtcgtacgg caggcccagc tccagaccaa tcttacgcac ttcgtctttg
    11881 aacagctctt tcagcggttc aaccaggccc atcttcatct ctttcggcag gccgcccacg
    11941 ttgtggtgag atttgatgac gtgtgcttta ccggttgcag aagccgcaga ttcgataacg
    12001 tcagggtaga tggtgccctg cgccagccac ttcgcgtctt ccagtttcag cgcttcttca
    12061 tcgaatactt caacgaaaac gcgcccgatg attttacgtt ttgcttccgg atcgttttcg
    12121 ccagccagcg ctgacaggaa gcgatcttct gccggtacgt gaacaatgtt aagaccaaag
    12181 tgatcgccaa acatatccag aacctgctct gcttcgttga gacgcagcag gccgttgtcg
    12241 acgaatacgc aagtcaggtt tttaccgata gcgcggtgca gcagcattgc ggttacggag
    12301 gaatccacac caccagagag gccgaggatg actttatcgt cgcctacctg ctcgcggatg
    12361 cgagctacag catcgtcgat aattttcgct ggcgtccaca gggcttcaca ctggcagata
    12421 tcacgcacaa aacgctccag catgcgcata ccctggcggg tgtgagtcac ttccgggtgg
    12481 aactgtacgc catagaagcg tttttcttcg ttagccataa tggcaaacgg gcagctttcg
    12541 gtgctggcta cggtgacgaa gtcggacggg atagcggtaa ctttatcgcc gtggctcatc
    12601 cagacatcga gcagcggttt accgtctgcg gtcagcgcat cttcgatacc gcgaaccagt
    12661 gcgctgtcgt ttacgacttc aacctgcgcg tagccaaatt cacgttcgtt agaggcttca
    12721 acgtgaccgc ccaactgcat tgccatggtc tgcatgccat agcaaacgcc gaataccggt
    12781 acgcctgctt caaagacata ctgcggcgca cgcgggctgt tttcttcggt ggtgctttcc
    12841 gggccgccgg aaagaataat gccgcttgga ttgaagtcac gaatttgtgc ttctgtcaca
    12901 tcccacgccc acagttcgca gtaaacaccc agctcacgca cgcggcgcgc aaccagttga
    12961 gtgtactgag aaccgaagtc cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt
    13021 gacgcttatt ccgaggcaag tgaaacagat aatataaatc gcccgacatg aagtcgggcg
    13081 aagagaatca ggagcccaga cggtagttcg gggactcttt agtaatggtc acgtcgtgaa
    13141 cgtggctttc ctgaatgccc gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt
    13201 cgtcgatagt accacagccg gtcagaccca tacaggagcg caggccgccc atctgctggt
    13261 gaatgatctc tttcaggcga cctttataag ctacgcgacc ttcgatacct tccggcacca
    13321 gtttgtcggc agcgttatcg ctctggaaat aacggtcaga ggaacctttg gacatcgcgc
    13381 ccagggaacc cataccacgg taagatttgt aagaacggcc ctggtagagt tcgatttcac
    13441 ccggagattc ttcagtaccc gccagcatgg aacctaccat caccgcgctt gcgccagctg
    13501 cgatagcttt ggcgatgtcg ccggagaagc gaataccacc atctgcgata accggaatac
    13561 cggtgccttc cagggcttca actgcgtcag caacggcagt gatctgcgga acacctacgc
    13621 cagtaacgat acgagtcgta cagatagaac cagggccgat accgacttta accgcgctgc
    13681 aaccagcttc tgccaaagcg cgtgcacctg cagctgttgc cacgttgccg ccgatgattt
    13741 gcagatccgg atatttagca cgggtttcac ggatacgctg caaaactcct tctgagtgac
    13801 cgtgggagga gtcgatcagc agaacgtcaa cgccagcggc aaccagcgcg tcaacacgct
    13861 cttcgttacc cgcacctgcg ccaactgcag caccaacacg cagacggcct tgctcgtctt
    13921 tacaggcgtt cggtttacgt tccgctttct ggaagtcttt cacggtgatc atgccgatca
    13981 ggtggaattc gtcatcaacc accagcgctt tttcaacgcg tttttcgtgc atttttgcca
    14041 gcaccacttc acgggcttca ccttcacgca cggtgaccag acgctctttc ggcgtcatgt
    14101 aaacgctaac cggctggttc aggtcggtaa caaaacgcac gtcacgaccg gtgataatac
    14161 ccaccagttc gttttcttcg gtaacgaccg gatagcccgc aaaaccgtta cgctcggtca
    14221 gttctttcac ttcgcgcagc gtcgtggttg gcagaacagt ctgcggatca gtcaccacac
    14281 cagattcgtg ttttttcaca cggcgaactt cttctgcctg gcgttcaatg gacatgtttt
    14341 tgtggataaa gccgataccg ccttcctgag ccagagcaat agccaggcgc gcttccgtta
    14401 cggtatccat tgctgcggaa agcataggga tattcagacg aatagttttc gtcagctggg
    14461 tgctgaggtc agcagtattc ggcagaacgg tagaatgagc aggaacgagg agaacgtcgt
    14521 caaacgtcag agcttcttta gcgatacgta gcatgggcaa tatctcgacc agagtggtta
    14581 ataaatattg ccgcggcatt atacagagcg taaccgattg catctacccc tttttgcaaa
    14641 aaatgcttgc tatccccgaa gggcgggtta ctatcgactg aataacctgc tgatttagaa
    14701 tttgatctcg ctcacatgtt accttctcaa tcccctgcaa tttttaccgt tagtcgcctg
    14761 aatcaaacgg ttcgtctgct gcttgagcat gagatgggac aggtttggat cagcggcgaa
    14821 atctctaatt tcacacaacc ggcttccggt cactggtact ttacactcaa agacgacacc
    14881 gcccaggtac gctgcgcgat gttccgcaac agcaaccgtc gggtgacctt ccgtccacag
    14941 catgggcaac aagttttagt tcgcgccaat attacgctct acgagccgcg cggcgactac
    15001 cagataatcg ttgagagtat gcagccggcc ggtgaagggc tgctgcaaca gaagtacgaa
    15061 cagctcaaag cgaagttgca ggctgaagga ttgttcgatc tgcaatacaa aaaaccactt
    15121 ccctcccctg cgcattgcgt tggtgtgatc acctcaaaaa ccggtgctgc gctacatgat
    15181 attttgcatg tgttaaaacg tcgcgatcct tctctaccgg tgatcatcta ccccaccgcc
    15241 gttcagggcg atgacgcgcc ggggcaaact gttcgcgcca ttgaactggc gaatcagcgc
    15301 aatgagtgcg acgtgttgat cgttgggcgc ggcggcggtt cgctggaaga tttatggagt
    15361 tttaacgacg aacgcgtagc gcgggcgatt tttgccagcc gcattccggt cgtcagcgcc
    15421 gtcgggcatg agacggatgt gaccattgcc gattttgttg ccgatctgcg tgcgccaacg
    15481 ccgtctgccg ccgctgaagt agtgagccgt aatcagcaag agttactgcg ccaggtgcaa
    15541 tcgacccgtc aacggctgga gatggcgatg gattattatc tcgccaaccg cacgcgtcgc
    15601 tttacgcaga tccatcaccg attacagcaa cagcatccgc agctccggct ggcacgccag
    15661 caaaccatgc ttgagcgcct gcaaaagcgg atgagctttg cgctggaaaa tcaacttaag
    15721 cgtaccgggc aacagcagca gcggttaaca cagcggctga atcagcaaaa tccacagccg
    15781 aagattcatc gcgcgcaaac gcgcattcag caactggaat atcgtttagc agaaaccctg
    15841 cgcgtacagc ttagcgccac gcgtgaacgt ttcggtaatg cagtaacgca cctcgaagcc
    15901 gtaagcccac tgtcaacgct ggcgcgtgga tacagcgtta ctactgctac tgacggcaat
    15961 gtactgaaaa aagtgaagca agttaaagcg ggtgaaatgc taaccacacg tctggaagac
    16021 ggctggatag aaagtgaagt aaaaaacatc cagccggtaa aaaaatcgcg taaaaaggtg
    16081 cattaagcca gcacgaattc aacgcgtttt ttcgatatca agccatgccc gtgctgacag
    16141 aagtaatcta ctgcaccgca ggcttttaac acctgcaacg gttgatggca gtctggacaa
    16201 agagctttca tttcaataat ttccccgcag gatgggcaac gcgcatggcc gttatcctga
    16261 tcaagcacat gctgacattg tggacaatgc agttccatat gaattcctcc ggtagccatc
    16321 cctctacatt catagaggga atggcagata aaatacttac ggataattat ttatttttct
    16381 tgatgtgctt catcagacgc ttacgtttac gcatctgggt tggcgtcagg gtgttacgct
    16441 tgttcgcata cgggttttcc ccttctttga actgaatacg aatcggcgat cccattacgt
    16501 ccagcgattt gcggaagtag ttcatcaagt agcgcttgta ggaatcaggc aggtctttca
    16561 cctgattacc gtgaatcacc acaatcggcg ggttataacc accagcgtga gcatatttca
    16621 gcttcacacg acgaccgcgt accagcggcg gttggtgatc ttcaacagcc atcgtcatga
    16681 tgcgcgtcag catagaggtc cccacacgac gggtggagct gtcatacgct tcacgtactg
    16741 attcaaacaa gttaccaaca ccactgccgt gcaaggcaga gataaagtgc acacgagcaa
    16801 aatcgataaa gcccagacgg aagtccagcg tttctttcac ctgctctttc acttcctggc
    16861 tcaggccatc ccacttattc accacaatga caagtgaacg cccactattg agaataaagc
    16921 ccagcagcga gagatcctga tcggaaatac cttcgcgcgc atcaatcact aacatcacca
    16981 cgttggcatc ttcaatggcc tgcaacgttt tgattacgga gaatttctct acagcatcgg
    17041 tgattttgcc gcgtttacgt acgccagcgg tgtcaatgag cacatactca cgtccatcgc
    17101 gttccattgg aatgtagata ctgtcacgcg tcgtgccagg catgtcataa acaacaacgc
    17161 gctcttcacc aagaatacgg ttagtgagtg tagacttacc tacgttcgga cgacccacaa
    17221 tcgccagttt gatcggcaga ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt
    17281 tctcttccgc ttcaaattgc tcccagtatt cagcgtattc gtcgacttcc tcttgcggtg
    17341 cgagatcttc catccacggc agcagcacat gctccagcag acttaatacg ccacgaccgt
    17401 gagacgcggc gatcgggtag atttcaccta aaccaagcga gtagaaatca accactgcct
    17461 gatcgggatc cagaccgtca gttttgtttg ccaccaggaa ggtcggtttt tcacgggagc
    17521 gcagatgttt ggcaatcgct tcatctgccg gcatcaggcc cgcgcgcgca tccaccataa
    17581 acagtacgac gtccgcttct tcaatcgcca gcagcgactg ttccgccatg cgggtttcta
    17641 caccgtcttc tgtgccatca atcccgccgg tatcaataca gataaactca cggccttcaa
    17701 tttccgcacg accgtactta cggtcacgag tcagacccgg gaaatccgca accagcgcat
    17761 ctcgggtgcg agttagacgg ttaaataacg tggattttcc tacgttaggg cgcccgacaa
    17821 gcgcgaccac aggtaccatg tttaaagcct catttttata aatcatcaga caacgcacgc
    17881 tatattcgcg tcgttgttaa aaacaggaaa acggcccctg tccaggagcc gttttcaaag
    17941 tgaacgacag agacgattaa cgtgtaatag agtacacggt tccgtctttt gcctggatca
    18001 gcagtttgcc gtcagcggca accggttcag tctggaaacc ggaactatca actttttgct
    18061 gggcaacgaa acgaccatct tcgacgttaa tccagtgcag ataaccttca ctgtcaccga
    18121 ccaccaggtt gccattatac agcaccggag aagtcagcag gcgatgcagc agatcgcttt
    18181 gtgcccacag cgtaacgccg ccatcaatgg tcaacgccat cacccggtca ttttgatcga
    18241 ccagatagat gcgattgccg tcgacgatga aatcattcac cgaacccagt tcgcgtttcc
    18301 acataatctg accactgcgc agatcaagcg ccgtcaggtt accattatag gccagcgcga
    18361 aaacaacgcc gttaacaacg acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg
    18421 tagaaccggt cgcctgggaa atacgctgct gccaaatcat ctggccctgt tccatcagca
    18481 ctgcgctgac gcgaccatta tcgcccccca cgacggccgc accaaaagcc gttgccggcg
    18541 cagactcgcc acgcaaagag agcgaaggca tatcgaggtt aactgtccat ttgacagcgc
    18601 cgtcagcttc gttcagcgct tgtaactgac cgttactggt gtggattaac accagaccgt
    18661 cgctgaccac cgggcgcgaa agtgcttcac ccgcgacttt agtttgccat gccacagtac
    18721 catcgctggt attcagcgcg taaacctgcg ccttttcgct gccaatgtag acatgcccac
    18781 cagacacggt cacaccgcca gaaagtaatg caggctcttt agagaaccag ccatctttct
    18841 cggccaggct gacagaccag atttctttgc catcatccgc attcagcgct tttaccaaac
    18901 cagcgcggtc cgctgcatag acaacgttgt ccgccagtgc cggatgaaga ttggaataga
    18961 agttgccaat gccgctacca acggaagtac tccacgccgt ggtcggcgta aactggtttt
    19021 caacggttgg caatggggac atctttacca catcttcttc gctgttaaac agcgaacagc
    19081 cgcttaaaag ggtaacggaa agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt
    19141 ccctctcaga tggacaaatt attaattttc atctgcatca tttcgctcag tgccggagta
    19201 acatcgcttt tcacgcctgc ttcccatgca ctacgcgcac cttgcttatc acctttgctc
    19261 agcaatgctt caccacgcag gtcggcaaca atggcagccc acccttcacc tttgatggta
    19321 tcaagggttt tcagcgcggc atcagcctgc ttgagctgta cctgaacgcg agcaagacgc
    19381 agatttatca cggctttgag attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg
    19441 gcggcagctt tctctagttc atttttgtca acaaattgct gcgcaagttc caaagaagcc
    19501 agcgcaccat aagtattttt attttcagca gcaaattttt ccgccgccgg gatgctatcc
    19561 ggtttgcctt cgctcactgc ggtaaccgca ttttgatagg caagagaagc ggagcgtgca
    19621 gaatcaacct gatggctgtt ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc
    19681 accccaacag ccagtgcttt gccattttca gcaaaaaagc gtttaaccgc ttctacctgg
    19741 tcgttttcgt tctcgtaaat ttccacgctg tccttctcct tccttaaccc agtaacgtgc
    19801 gcaaatgcgc ggctacgcta tcctgcgcaa ctgccgtttg ctcaccagag cgcaaatcct
    19861 tcactactgc tgtgccgtta gccacttcag actcacccag caccacagca acgcgggcac
    19921 cccatttatc agcacgggca aactgtttct taaagttgcc gccgccgtgg ttggtcatca
    19981 atttcacgcc cggtaattca tcacgcagac gctcagctaa tgccatagcc gcagattgtg
    20041 tatcagcacc tgaagccacc aggtatatat cgacaacagg atcggcttta aattccggat
    20101 taacggcctg tactaacaat acaagacgtt cgaggcccat cgcaaaaccg acagccggtg
    20161 ttgcacgacc gcccagttgt tccacaagac cgtcataacg accgcctgca cacacggtgc
    20221 cctgggagcc gagactgtta gtcacccact cgaaaacggt acggttatag taatccagac
    20281 cacgcaccag acgctggttt acggtgtaag cgatccccgc gctctcaagc agtttgcaca
    20341 gaccggcaaa atgctcacga gattcctcgt ccagataatc acctaatgcc ggagcgtcgt
    20401 tgagaagcgc ctgcacttcc ggattttttg aatccagcac gcgcagcggg ttagtgtaca
    20461 tgcggcgttt gcagtcttcg tccagctttt ctttatgctg ctcaaggaat gccaccagcg
    20521 catcgcggta attggcgcgt gcttccagcg aaccgataga attcagctca agagttacgt
    20581 gttcggaaat acccagcgcg cgccaccagc gggcggtgag cataatcagt tcagcgtcga
    20641 tatccggacc ttgcagaccg aaaacttcgc agcccaactg atggaactga cgataacgcc
    20701 ctttctgcgg acgctcgtga cggaacatcg gcccgatata ccacagacgc tgttcctgat
    20761 tgtacagaag accatgctcg atgccggcgc gtacacagcc cgccgtccct tcagggcgca
    20821 gagtcaggct gtcgccattg cgatcctcaa aggtgtacat ctctttttca accacgtcgg
    20881 tgacttcacc aatcgcacgt ttgaatagcg gggtctgctc tacaatcggc aagcggattt
    20941 cactgtaacc gtagctgccg agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg
    21001 ccgtttcgcc aggcaggtaa tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt
    21061 ttattctctt tctgaatata aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagct
    21121 atgcgggttc aatcatacac gggaagcgag gcgcttccca tcacgttatt atttttcaac
    21181 ctgctgaacg tcaattcgac gcgcttcgtc cagctgactg gctttcgcac gaatgcgtgc
    21241 ttccagctgg tcgatcatat cgttgttgtc cagacggtct ttgcgcacgc catcttcata
    21301 gaggccgctt ttcttgttgc cgccggtgac gccgagtgta gaaaccagcg cctcacctgg
    21361 gccattcacc acgcagccga taatcgaaac gtccatcgga gtgatgatat cttccaggcg
    21421 ttgctccagc gcgttaaccg taccgataac atcaaattcc tgacgcgaac aggtcgggca
    21481 ggcgatgaag ttgatcccgc gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac
    21541 tttgatctct tcgaccggat cggccgccag tgatacgcgc agcgtgtcgc cgatgccttc
    21601 agacagcagc agacctaaac caatggcgga ttttactgcc ccgctgcgtg caccaccggc
    21661 ttcggtgatc cccagatgca gcggctgatc gatctgtttt gccagcaaac gataagactc
    21721 aacagcgagg aagacgtcag acgctttcac gctgactttg aactgatcga agttcaggcg
    21781 atcgagatga tcaacatggc gcatggcgga ttccagcaac gcctgcggcg tcggttcgcc
    21841 atacttttct tgcagatctt tttccagcga tccggcgtta acgccaatac ggatcggaat
    21901 gtttttatcg cgcgcacagt caaccaccat gcgaatacgc tcttcattac cgatattgcc
    21961 agggttaata cgcagacaat cgacgccgta ttccgctact ttcagtgcaa tgcgatagtc
    22021 gaagtggatg tcagccacca gcggcacgtt aacctgctgt ttgatgagtt tgaacgcttc
    22081 tgccgcgtcc atcgtcggta cggagacacg gacgatatca gcgccaacgc gttccagcgc
    22141 cttgatttga ttgaccgttg cttcgacgtc tgtcgtacgc gtattggtca tggactgtac
    22201 ggcgatggga gcaccatcgc caatcggcac attcccaacg taaatacgtg ttgattttct
    22261 acgttgaatt ggagcctggt tatgcatgaa aaatctcccg cgttacccgt ctgttactgc
    22321 gccggtgatt gttcggcatt gagggtcaga cgcgcaacct ggttagttct gataaaacga
    22381 ctcagatcga caggtttccc ttgatactgg atctgtactg cggctggcgc accaattttc
    22441 agtttgtatg gtgcctggcc ggttaagttc aaattaccgt ctttacgctg cataccgcta
    22501 aacaattttt taccggtagc atcagtaacc tccagccagc aatcggcagt aaagttcatc
    22561 accagcgcat tcggatcagc cgccggcgta gtcacgcctg cctgatcggt tggcaacggc
    22621 gcagcgccat ctggcgttgt tgttgccgtt ggtgccgggg tcgcggcggt atcaacattt
    22681 gcctgcgaag gcgaaacaac cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt
    22741 acggcaggtg tttgcgtgtt ggttgcggta gtatccacag acgctggcgg cgtggacgtt
    22801 gtagccgggt ctgtagttgt cgacgtattt aacggaacac tctgcccctg ctcgctatta
    22861 ctgctcagtt ccgccgaaga ttgatcggcc atagtggtga tctcttcctg ctgagctttg
    22921 tggtcttgcc accaccaggc accgctcagg ccgataacca caaacaacac cagccaggtg
    22981 aaggtcatca gccagccgtc acgttttttg cggcgtttac cgagggaaaa actctgcata
    23041 ggcgcaactt ttgcagcccg tagtggagcc tgcttttcca gccctggcag cagttcttct
    23101 tctggaatat gtaccagacg cgcataagag cggatatatc cgcgcaggaa tgttgaagca
    23161 agatcggcgg gtgccttatc ttcttcaatg tcgcgtaccg tggaaacctt caggcaaagt
    23221 cgctcggcaa cggcctgctg actaagtcct agttgttcgc gagcattacg caggcgagcg
    23281 ccggtagtaa gtgcttcatt ttggtcgtgc gtggcttcag tattcattcg ctgcaggtac
    23341 gtttaaatga gaattgagat gccggtgaac catcatgctc acccacaccg cgaaacatcc
    23401 gttaagttaa ccgttatcat acagtataag accgtctgtg tgctcgtgac aaagcccaca
    23461 ttcactaagg ctaacttact gttgcatcgt tacatactgc cttaaagtca gcaaaaacgc
    23521 accgttaata ttgaccagac aattgcaact taatacttca ttcataaaag attacgccac
    23581 ggtacataaa gtaaccgtgg cgtaatggct atcagaccgc tttaatgtcg atggcttcac
    23641 cctgcatccg tttacgcagg gtacgtttcg tacggtcgat aacatcgccc gccaactgac
    23701 cacaggcagc atcgatatca tcaccacggg ttttacgcac aatagtggtg aaaccgtagc
    23761 tcatcagcac ttttgagaaa cggtcgatac ggctgttcga gctgcgtcca tacggcgcgc
    23821 ccgggaaggg gttccacggg atcaggttga tcttacacgg cgtatctttc agcagttccg
    23881 ccagttggtg cgcgtgttca gtgccgtcgt taacgtggtc aagcatcaca tactcaatag
    23941 tgacacggcc ctgattggcg ttggatttct ccagataacg gcgcaccgca gcaaggaacg
    24001 tttcgatatt gtactttttg ttgatcggca caatttcgtc acgaatttcg tcgttcggcg
    24061 cgtgcaggga aattgccagt gcaacgtcga tcatatcgcc cagtttatcc agcgccggaa
    24121 ctacaccgga agtagaaagc gtgacgcgac gtttagacag gccaaaaccg aaatcatcaa
    24181 gcatgatttc catcgccgga acgacgttgt tcaggttgag cagcggctcg cccatgccca
    24241 tcatcactac gttagtgatt gggcgctgac cggtgacttt tgctgcgccg acgattttcg
    24301 ccgcacgcca cacctggcca ataatttccg atacccgcag gttgcggtta aagccctgct
    24361 gggcggtgga acagaattta cactccagcg cacaccccac ctgcgaagag acgcagagcg
    24421 tggcacggtc gtcttccggg atatacaccg tttcgacgcg ctgatcgcca acggcgatcg
    24481 cccatttaat ggtgccgtcg gatgaacgct gttcttcaac cacttccggt gcgcggattt
    24541 ccgccacctc tttcagtttg ccgcgcaaca ctttgttgat gtcggtcatc tcatcaaagt
    24601 tgtcgcagca atagtgatac atccacttca tcacctgatc ggcgcggaag ggtttttcac
    24661 ctaaatcttt aaaaaactcc cgcatctgct gacggttgag atccagcagg ttgatttttc
    24721 catctttcgt ggtgacgttt tcaggtgtga ctaattgttc agacatatgc tattccggcc
    24781 tcgttattac acgttatggc ccctggagga ttgaaaaaag aaacgccccg gtgagcggct
    24841 gctcgtccgg gggcgctgca ttgtacaaat tctggcgcac ggatgccacg tttgcacgcg
    24901 gcatttacga aattattaac gggtgcgcgg gcacacttcg ccttcgccaa agaaataagc
    24961 gatttcgcga gcggcagatt cgacggaatc agaaccgtgg gtaccgtttt cggtcaggct
    25021 gtcagcgtaa tcagcgcgca gagtaccagc cagtgcgttt gccggattgg tcgcgcccag
    25081 cagatcgcgg tgacgctgaa cggcgttttc accttccagc acggaaacca cgatcgggcc
    25141 agaggtcatg aattcaacca gaccatcaaa gaacggtttt ccatcgtgtt cagcataaaa
    25201 gccacgtgcc tgttcaacgg tcaggtgcag cattttggtg ccgacaattt tgaaccctgc
    25261 agcttcaaag cgcgcaaaga tattaccaat gacgtttttt gctaccgcgt tcggtttgat
    25321 gatggaaaaa gtacgttcaa tagccatttt tacctctgta aattgttctg ttgttgtctg
    25381 taccagcgta cgagatggcg cggattataa tgagcaacag ggccgttgac tattgatgaa
    25441 ggtaacattt ttttaaaata aaatgagttt tagcaacaat cagggacaaa acagactatt
    25501 gcatgacaaa tttcactgtc gcgatttgcc ccacatcatc catcaccagc aattgataat
    25561 cacctttatc cgtcaaatgc agggtcacgt tgcgcccgcg ttcagttaac ggttcgccat
    25621 tcagaaacca ccagcgttca cctgcccctc cacttgattg caacggcaaa gtcgcttccg
    25681 ctgcgcccgg taaacgttta ataatcgcgc catcgcgaac gcctgtcagc tgcagaggga
    25741 gctgggcatc gtgaccgtat gacgggcaaa ttgtcgaggc cggtggtaag cgcacagcgc
    25801 gacgttctga tgcgggcagc cagggttcca gcggtagcgg ccagacattt atcatttctt
    25861 gtcgcgcttg cgggcaatca gcggcaacac gtttgccatt ttcatccagc cagatgggga
    25921 aacgaatgcc attaatgcct tcctgatccg gcagtaatag agttggcggc tgactcccgt
    25981 ccagcagcca ggttgccagg cggcggcgac agttaccgtc accttccggc aaagactgtc
    26041 cgcccggcca gcagataacg ccacgagtga ctgagttcgg gcgcgggtct tccggcagat
    26101 tcgcactgcg cgacagtaag atattattga cctgattcaa caatggtacg gcactggcaa
    26161 agccaaactg accaacaacg ggcgtgccgt ccggtctgcc agtccagatc ccaatgacat
    26221 agcgagcgtt aaccccaatc gcccaggcgt cacgatagcc atagctggtg cccgttttcc
    26281 atgccagtgg ggcgacgcgc ggcaaggcac tatccggcaa gggtttcgct tcatcagcca
    26341 taatccggcg aatgatccac gccgcccccg acgacattaa aggccgttca agcagcggat
    26401 cgtcaggctg taagcgcaat ttgcctgcct tgccgtggcg agcaaacgcg gtatacgctg
    26461 ccgccatatc ttccagtttt gcaccagcgc cgccgagaat gagtgaaaga ttcggcgcag
    26521 caccgttggg caaatataac ggcaatccaa cattgcgtaa ctttgccgca aaccgtttcg
    26581 gtccataggc ttccagcacc tgcacagcag gtaagttcag cgagcgcacc agcgcctcgc
    26641 tcatgctgat cgggccatga aaaccgctat caaagttacc tggtcgataa tcaccggtgc
    26701 gccgggggac gtcttgcagc agtgatgccg ggtggatcaa gccttcatcc agcgccagac
    26761 cataaacaaa cggtttgagc accgatcctg gcgatcggat cgcattgacc atatcaacat
    26821 gaccaaagcg tgaatcatcg ttgagatcaa ccgatcccac ccagccgcga acacgcatat
    26881 cggtatgatc aaccacgatc atcgccagtg agctgcgcgg tggcaaccgc cctttccagt
    26941 tttgcgccag ttcttccaga cgtcgttgaa gaccggcatc cagcgtagta acgattttgt
    27001 cgcttttgct tttaccgagc atcatgcgcg aaaacagcgg tgccagttgc ggcatttgtc
    27061 ggggtgccgg ccagatgggt tcttcccttg actcttttac ctgctcacgg gaccacacac
    27121 cttgcacggc catccgttcg agcactttat tacgcgcggc ttcggcacgc tccggccaac
    27181 gatccgggcg aagacggctg ggcgcttgcg gcaaaaccgc cagcattgcc gcctcggaat
    27241 agcttaaatt cgcaggcgac tttccgagat aagcccaact tgccgcaccg atcccctgca
    27301 acgtaccgcc aaacggagcg cggttaagat acaaggtcag aatttcacgc ttagacagat
    27361 gccattccag ttgcaacgcg cgccagagct ggcgaatttt gccgccaaat gttttggggt
    27421 gaggatcaag cagacgagca acctgcatag tgagcgtgct gccaccggaa ataacccgtc
    27481 ccgaagtgag atcttgccat gctgcgcgcg ccaccgagaa tggattcacc cccggatgct
    27541 tccagaacca gcgatcttca taattgatca gcgcttcaag gtaacgtgga gaaacatctt
    27601 cgattgttac cggataacgc cagatgccgt cagcatcggc gaagcgccat agcggcgtac
    27661 catcctgcgc cacgaccact cgtgcgggat tgacttcatg cagcggtaga ggccagagtt
    27721 tatctgctcc ccacgctgca agaaaaagga gaaatggcgc agctgccagc gttatccagc
    27781 agccgcgttt ggttaacaag cgaggcattt acggtcggac aatcagcaga tcttcagccg
    27841 cgccggtcgc ccgccattgg ggaacataca ttgattccac catcggttgc ggtacctgat
    27901 acgttcccgg cgtcaccgcc cgcgccagat acaccagcgt taccggttgg tattcatcaa
    27961 cggcaaccgc cgccacaaag cgatcgtcac ggaactcaat gtgcttaata ctcgcctgct
    28021 gcatctggtt cagtaagttt tgcacttcgc caccactttg ctccaggctg gcgctaccgt
    28081 tcgccagatt ctgattttcc agttccagac ccgcaggcag cagatccacg actaacgcat
    28141 ccggcacgct gttactggct tttacctgca accagaccag caccagatcg ccgctacgta
    28201 acgagtccag cgatttgctc ttaccatcag taccaagaat atgacgctcg atttgcagca
    28261 cattgttcgc cggtaaaggt gcggattgcg gataaccgct ggcatccaca cgcagccata
    28321 acggctgatc gccactgttt ctcacctgca aggtggcaag ttgatcgcta ttcagattgc
    28381 tgtttagcgt tttctcgcct gtcagcggct cagctgagaa agaggtttgc gcctgccatt
    28441 tacccggtaa atcctgaatc gtacgggcag ccaggaacaa cgcgttactt tcctgcgtcg
    28501 atagccagcg ttcaccaaat gcctgctgcg aaagcgtgtt cagcaaagtg ttctgctcat
    28561 cgggtagcag tttattttct tccagcaagt agagcattaa cgcgttgtcg cgcagtggac
    28621 taccgtaatc acccagccat atccgctcat cactatttct cggcgttttc agcgccagca
    28681 caatcgcttc ttcaccacgc gttgcatcac ccatggtttt cagcgcaacg ccaagttgca
    28741 gcagcggtaa accagaagcg gcatctgcac gatgctccca gatttcacgc agcgcaccca
    28801 gtggtgcttt ttgctgacgg gccagcacca gcgcagcgta agactgaacg gcgaatttac
    28861 tggctttgag attatccgcg tacgggatcg acatcatgcc cggatcttgt aaatagcgca
    28921 gcagacgctc attaccccgg ttaatggcgt ctgtcggcac gctgtaaccc tgttcgcctg
    28981 cgcggaccag gaaatccatc acgtaagccg tcagccagta ctcttcgtca ccgtttttat
    29041 cccacagcgc aaagccgccg ttatcacgtt gcatttgcag caaacgggaa atgccgatat
    29101 cgaccgatgc acggcgtttc tcatcactgt cgcctttgat gcccaacgcc tgcagttggg
    29161 ctgcgttggt ataaagtgac gggaacaggc cgctggcggt ttgctcaaga cagccgtacg
    29221 gatacgcttt taactctttg atataacgtg caatgttcag cggtggtttg ccgctcaaca
    29281 acaattgccc ttccagcgta acaggcgaga agttttgcaa tccatccgcc ggaatcgccc
    29341 atgtctcacc aggctgtaac gccgtaccgt aattaaccgt ttgtgccggg aacgccggac
    29401 ggacgccgat tttccactgc ttatgctgat cggcaacggt ttcacccggt aacgctaacc
    29461 cgctaatggt ggcctgaatt tctccatcgc cataacccgg caatgctcgc accgggataa
    29521 acagcgtagt acgcacacct ggcgctaatt caacggctgc gggtgaatcg ctgaccagtt
    29581 caagcaaacc actggcggtc agggcaacgt tcagtttttg cggtttatcg gtaagattag
    29641 tgatatccag cgtcagacgc gaggtatcgc cactcgccat aaagcgcggc atgttcagtt
    29701 cagcaatcac cggtgcggca actatcactt tgctttcgtt gctaccgaaa tcatctgccg
    29761 tccaggcttg cgccatgacg cgcaattcac cgttaaaatc gccaatcggc agtgtaaccg
    29821 agccttcgcc ctgttcgttg agcgttaccg gcagcgcctg ctgcgcgaca atattgacgt
    29881 gattgaccgg cggtttacca ccacgtttca gctcatcacc atcgccaccg aaacgcagag
    29941 ctgccagacg cccctgacct tcaataacct gaccgtaaat atcgtaaatg tctgcgccat
    30001 agcgtttctg accaaagaac gcctgccacg gatctggcgt gacgtagtca gtaatattca
    30061 gcacaccact atcaacggca gacaccagca cattcacctg tttaggcttc tcgccatttt
    30121 tagtgctggc tttaattttc acggttaatg gttgattggg acgcattttt gctggtgttt
    30181 ccagcgccag atcgaggcga cggttttcat cgccaagcgg cagatgcaac acaccaaccg
    30241 cgcgttttgg cgtcgcggag cgagatttat cgccaggacg taccaccagc gtacttaaat
    30301 acagatcatg acgattccag gttttatcga ccggaatcgt cagatccagc ccttgagccg
    30361 gaacatcaat ctcttgccac cacagcggcc cttcactgga ctcgaccatc gcataacctt
    30421 tacccgccgt tggcgcggcg atatgcaact taatggtgtc gccagggcga taactggctt
    30481 tatccagttt cagcgtgaca cggtcgggtc gcactgcgcc gctaccgtcg ctgttgtcct
    30541 gccagctata gccagcccag aaacgaacac tactgaccgc ttcattcggc gctttgactt
    30601 ccagacgata agcgccccac tctaccggaa aactgacctt gccggtttcg tccgctttca
    30661 gatccagagt ttgttcattt tcgatcagat ctttttgatc aaactgtgac tgccagcctt
    30721 catcttctga ccagttccag tagtaatcgc ggcgttcgcg aatcaggcgc acctgcaagc
    30781 ccgacacggc ttttttcacg ccttgcgcat cgctataaac gatgtcaaaa gcggcgttac
    30841 tgccttcatc aacaatgggc tgtttcaccg tgctgtcagt acgataatcg taaaccgatt
    30901 tcgaggcgaa ctgcggacgg atccccggca atgcatcggc aggccagata gcctgctcag
    30961 cgcggcgcgt caccgggcga ccgcccgatt ccagcaaact accctggaaa ataacctgta
    31021 atggggaatg cgtttccttc cactggcttt ctgtagaaac ttcgccgcgc cctttatcat
    31081 ccagcgtcaa ctgaacttca tccagcgtgc gggaaagatt ttcggcagct atatcgccga
    31141 attcaaaacc aggtaaggct gacacagctt cacgcagtgg gcgcaggaaa agttgccctt
    31201 gcaaagtatt accattagca ggtgcaccat acaggtagta ccccaccacg gagaatttca
    31261 cttcatcttt cggcgttagc ggggttttct caccggtcag attcagcgcc atgcgctctg
    31321 gcataaaatc ttcgacgtgg aaatcccaca tccgatactg gttatcgccc gtgttagcgc
    31381 gaatatgcca cataccggtt gccgcattgc tatcgagtgg ccaggtaaag tggtagaggc
    31441 cattctccgg ctgactaacg acgctcctga gtacctgccc atcgggttta atcacgtcta
    31501 acttgatggg ttgattgggc aacgctttac cgtctgcatc acgcagcaaa ccattgagga
    31561 ttaccgtttc acctgggcga taaagatcgc gtgggccaaa catgaaaaac tgtttgctat
    31621 agcctggcgc gccagcaatg ttaaattctg ctaagtccag cgccggaagt tttaaatcga
    31681 gtagcgttgt ctgaccgtct ttacgcgcca acagtaatgc cgcgttttta tcattttcca
    31741 gctgcacatg cccctgagcg tcactggttg cctgagtcag agtctgccct ttctcattta
    31801 ataaagagac ttcaattcct tgctgggccg cgccgttttc cagactttgg gtaaagatat
    31861 ccagacgatt gtgataacgg tgagctgaaa cgccgatatc acttaacgta aacagcgtcg
    31921 cgggattact gtaatcgtaa cgtccagcct gattcatcac agccagatac acgcccgcct
    31981 gctgaagcgg tttgatatcg cccagcggca gcaataattt ttcacgggtg ttacgcgcag
    32041 gattgagatc aaaccgtccg gtgtagacca gatccgccat ctgcagcagt ttgtctgact
    32101 gccagttcgc cagcgaattg cggtattccc attggctaat gaatgctggc agagattctg
    32161 gcttaacgcg gaagaagtta acatcgacat tattaacgtt gagcgccatt accggcagcc
    32221 cttcaacgac tttgccaggc agcagcgaac cacggctggc aaaaccgacg ctgggttgga
    32281 tgtcgcgggt agttatagtt ttttcgtaat ctttactgaa ggttgcgttg ttgagtgctt
    32341 tgacctcctt gccaatagta acgatcaaat cacgtttcgg ttcgaggtgg cgtaaacgta
    32401 actctttaag attatctgac agctcccagg caccatccac tttgccgctt tttttatcga
    32461 cgacatgaat aacgcgtgag aaatcctgat ccgggtcgag agggatggag aacgtcagca
    32521 ccagcgtggc tgcaccatca agttggactt cagagagatc cagcaatgtc agcaccttac
    32581 cggcactctg ttgggccagt ttttgtctct ccggcacgga gagttttgct gaactcgcgt
    32641 tttctgaaga ggccgcttta gtaacttcag aaggcgcatc ttttttcacc gctgttggcg
    32701 cgttatcgtt gttgtcgcac cctgccagcg ccagcattag catgcaggcg gctacgcgta
    32761 actttttcat ttttcatccc tgccacaatg gcccgttagc aacgtcgaat aattattatg
    32821 cgtgagaatt tacgttatgt aattcagtat cacgcccata caatccgaat tttagtgaga
    32881 attgtggctc agacagccaa atcagcctct ttcgcttgtc gcttttcact aaacatccga
    32941 caatttagcc taaccccggc aaaaatggag atgcctatgt ccacgacatg gtttgtagga
    33001 gccgactggc tcgccgaaca tattgatgac ccggaaattc agattattga tgcccgcatg
    33061 gcgtcgcctg gacaggaaga tcgtaacgtt gctcaggagt atctgaatgg acatattccc
    33121 ggcgcagtgt tttttgatat cgaagcgctt tctgatcaca cttccccgct tccgcacatg
    33181 ctgccgcgcc cggaaacgtt cgccgtggcg atgcgtgaat taggcgttaa ccaggataag
    33241 cacctgattg tctatgacga aggtaatctt ttctcagccc cacgagcatg gtggatgctg
    33301 cgcacctttg gtgtagagaa agtgtcgatt ctggggggtg gacttgcagg ctggcagcgc
    33361 gatgatctgc tgttagaaga aggtgcagta gagctgccgg aaggagagtt taacgccgcg
    33421 tttaatcctg aagccgtggt gaaagtaacc gatgtattat tggcaagcca tgaaaatacg
    33481 gcgcaaatta ttgatgcccg cccggctgca cgttttaacg cagaagttga tgaacctcgc
    33541 ccaggtttac gtcgcggaca tattcccgga gcactgaatg ttccgtggac ggaactggtg
    33601 cgcgaaggcg aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc
    33661 agctacgaca aaccaattat cgtcagctgc ggctctggtg taacggcagc cgtggttttg
    33721 ttagcactcg cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa
    33781 tggggcgcgc gggcagattt accggttgag ccagtgaaat aagtatttta caggcaataa
    33841 aaaaccgccg aatttggcgg ttttttattg ctggtctggt tcgcggcctt tccagcaggt
    33901 tgacttgtgt tacatgagca acgcaggtgc ttcacagcaa aacaatactc accagtaact
    33961 ctctttttgt caagcaaaag agagtaatta ttgtttattt agcgtattat cgacaccggc
    34021 cctttccgcc gtgttcggta ataaattaac ctggcttatt agtccgaatt cagacaaata
    34081 taaacaaatc ctgctcaaaa ttaaaaatac taaccggtaa aagatattac ttaaacatgt
    34141 aaattcactt tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg
    34201 tccggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg
    34261 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
    34321 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
    34381 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
    34441 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttctgtt
    34501 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
    34561 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
    34621 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
    34681 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
    34741 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
    34801 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
    34861 ggctgctcag cggatttccc tctccaccaa tcaacaaact gatttcacgc ggcataaatt
    34921 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
    34981 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
    35041 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
    35101 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
    35161 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
    35221 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
    35281 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
    35341 tgaatttgca gaatttgtag gccggataag gcatttacgc cgcatccggc atattagttt
    35401 acgccgttaa cagattagca atcgtgcgca caccaagtcc cgtagcaccc gcagaccact
    35461 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
    35521 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
    35581 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcga tggaattccg
    35641 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg
    35701 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
    35761 ccccggtgag ggtcgccgca tcaatgatca gttccggttt ctgcgcactg gcatcaatca
    35821 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
    35881 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
    35941 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg
    36001 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
    36061 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
    36121 ctggcgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
    36181 ctgaaccgcg tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
    36241 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
    36301 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
    36361 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
    36421 ccactttacg cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg
    36481 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg
    36541 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
    36601 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
    36661 taatcttcat cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc
    36721 agccccagga tttttccgct aaatgattcg cgttgcagga aggcggcaag tgagtgaagc
    36781 cccaggagca tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc
    36841 gtgaagcatg acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga
    36901 ggattttctc gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact
    36961 gatgcatatc ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg
    37021 cttcgccaat ttcgcggcta tcggtccatt taagtcccat actaacctct gttaatgctc
    37081 acgcgcatgg ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtgacgcg
    37141 cgcctggcag cttaaacggc tttccggctc cagtccccag gctttgtcca gcatgtcgtc
    37201 ttcctgctct gagctttccg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt
    37261 ggtgcaagca caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc
    37321 gtcgagaatg gtttcaccgc tattagcttc cagaacagcg ccatcagggc agagatcctg
    37381 atgaggcaaa ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg
    37441 cacgacgaac cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt
    37501 ttttaatcgc ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag
    37561 cggcagcatc gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca
    37621 gcgcgccgtg cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc
    37681 gggcttttac gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc
    37741 tatcggtcag accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct
    37801 ccatcgccgt cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg
    37861 caccaccagc cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt
    37921 cctgcaccag ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt
    37981 taaaggtggt gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt
    38041 tctccaccag gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca
    38101 gcatttcgct gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag
    38161 cgacgacttt atccgggtcg atggaagtca gaggtggacg accgaaaaat tcgcctaccc
    38221 gttcacgcac cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcat
    38281 cagcttctac acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt cgttttacca
    38341 gtggcgcgat cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt
    38401 taacggtcac ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca
    38461 gttcacgctg aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat
    38521 ccgccagcag atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca
    38581 cttcaaacac gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt
    38641 cataaacagc gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg
    38701 tcggttcgtt aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc
    38761 cctgacgctg ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc
    38821 ctgccagggc ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca
    38881 ccgggttcag cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga
    38941 attgataagg cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc
    39001 gtttaacaga actaatggtg ttggcggtat caagcgctgc gttagtacgc gcgtcataac
    39061 ccaccgaatg cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat
    39121 ggtcggctaa cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc
    39181 ccaggtcaat accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac
    39241 taatttgtaa taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg
    39301 agttgttcgg cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc
    39361 gccgcgtccc acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac
    39421 atctttttca cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc
    39481 tcgtccagct cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta
    39541 tgctgctcgc tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac
    39601 ggatgacgca gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt
    39661 tgttccgcct ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga
    39721 aaacgcaggc tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagagggtg
    39781 aagtaatcca taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa
    39841 caaaccccac gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc
    39901 tttcgccgca accacactca tctttaacgt tcgggttggt gaatttaaac ccttcattca
    39961 ggccttcttt tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga
    40021 ccacgacttt cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa
    40081 attccagtac ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa
    40141 accctttgcc gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg
    40201 taatcgacat aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca
    40261 atggcggctt tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt
    40321 tcaagttctt cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag agacttccct
    40381 ttcacccatt cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta
    40441 aaacgcgcgt cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg
    40501 ccacaggccg gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa
    40561 cccacgttac gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat
    40621 tctcctgatt ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc
    40681 tgcttgtaca tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact
    40741 aactcgatgg tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata
    40801 gagctatgtg ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg
    40861 cttgctgacg tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc
    40921 gactcacctt caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg
    40981 tcaccgttca ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg
    41041 ccgcgcagac gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc
    41101 attccgacga tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg
    41161 ccgtgcattt gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata
    41221 cctttcgggc catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc
    41281 aggtcgatag gcagtttacc cacgctctgg gttgcatcaa cgtgatagat aatgccacga
    41341 gcacggcaca tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg
    41401 tgcatgatgg aaacaaggat ggtgtcgtca cgcatcgctg cttcaagttc tttcaggtcg
    41461 ataatgccgt tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga
    41521 cggcaggtat ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt
    41581 ttctgataaa agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag
    41641 gtaaagacga tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata
    41701 tctaccgctt cttcagcctg ccagccgaaa cggtgagaac gagaggccgg gttaccaaag
    41761 gttccgtcca tcgtcatgaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg
    41821 gttgcggagt agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact
    41881 caatgcaagg aatcaggcta ccggctggat gtacgaccgt gtttacggag tatttagcac
    41941 tccggcctga ttctgaattc tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt
    42001 gtgcgggtgc gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg
    42061 ttattaacca gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa
    42121 tcacgccata gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga
    42181 caacgggtgg catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg
    42241 ctggcatctt tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca
    42301 tttttacgca gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg
    42361 gaaatatcag ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg
    42421 gtcacggcat agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc
    42481 ctgcccgggg ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat
    42541 tcccgagtaa attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt
    42601 gccatttttt tatcgtggcg tttgtacctg ttgtcggatg cggtgtgaac accttatccg
    42661 acctacggtt ctgtacattg taggcctgat aagacgcgtt aagcgtcgca tcaggcaact
    42721 actgttattc ggccttgtta cctttattct gctgctcaat agaagccaga atcccgcgca
    42781 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
    42841 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat
    42901 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
    42961 gctcgccgtt ttcctgagtt gccagccagg ccatgcgcac ttcataggca ataacctgaa
    43021 ccgccatcgc caggttcagc gagctgtatt ccgggttagc cgcaatcgcg acatgataat
    43081 ggcatttctg caactcttca ttggtcaagc cgacgcgctc acgaccaaac accagcgcca
    43141 ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
    43201 gccacggcag cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
    43261 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca
    43321 gggcaatcgc ctgggagtcg ggtttcacca gtggattaac cagccacaga ttggttaatc
    43381 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
    43441 gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt
    43501 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
    43561 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
    43621 gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
    43681 agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
    43741 acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg
    43801 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggtact accaacttta
    43861 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
    43921 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg
    43981 cacagctgaa cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc
    44041 tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
    44101 gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
    44161 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt
    44221 tcgcggcagg cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg
    44281 gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccacgtgtt gtgaaagcca
    44341 tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg
    44401 tgatgtcact cacccgccct cgcctttcag gcgctattct gaaatacttc ctcaccgctt
    44461 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc
    44521 aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct
    44581 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    44641 ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta
    44701 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg
    44761 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    44821 tggtcagttg gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    44881 gtaaatcaaa aggcacgccg tcccaggccg gattgctgga cgatacgcaa agcgccatca
    44941 atgtggtgcg ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta
    45001 ttggcggggc gaatattctt gctgttattg gtcagggtga tcgtgaaggc atacgtgcgg
    45061 tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca
    45121 gtggctactt acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga
    45181 tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa
    45241 agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca
    45301 ttgatgcttt ttccgatcgc cacggcgatg tttatcgcga acagatggtg gactttatcc
    45361 ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa
    45421 ccaccttaag acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga
    45481 cgcttgccaa catttctgat gattagcatt cccttcgtca tttccctgag caaactttag
    45541 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    45601 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    45661 ttgaccgcca ccacggcaac tttcagcgag cttaatggtg tggatgatga tattgccagt
    45721 cttgatatca gcgaaacagg acaggagatc ctgcgctatc atcaactcac actgacggct
    45781 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    45841 cactggctac gacgtggttt ccgtctgtgt ccatcattta ttaccagcca tttcaccccc
    45901 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttatga cgataccaat
    45961 ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga aacgcgcgat
    46021 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg
    46081 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    46141 caggaaattg gacgccgctg gcagcgtcgg gcgctccagc ctgtaccacc tgatgagcca
    46201 ctgtttatgg cactactttt ttccatgttg cgggttcccg atccactgcg ggatgcgcat
    46261 cagcgagaca gacaattgcg tcagtctatc aaacgtctgg taaactattt tcgtgagctg
    46321 ggaaatgttc gtttttatga tgaacagggg ttatgtgatc agctttatac ccacctcgcc
    46381 caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc
    46441 gccagactgt acccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt
    46501 gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc
    46561 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttcttcttac cgggaatgat
    46621 agcgagcgag aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac
    46681 attaagcata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    46741 attattgcgc cttataccat gccattaccg ctcttttcac caccgctgat ctatacggac
    46801 ctgacgctga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    46861 aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatta cccagaacac
    46921 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    46981 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc
    47041 agcaatataa cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg
    47101 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    47161 gcaaatcgcc gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact
    47221 cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    47281 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    47341 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    47401 cgctgaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcgggcg
    47461 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg
    47521 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    47581 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    47641 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    47701 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc
    47761 cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa
    47821 actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    47881 taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa
    47941 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    48001 tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt
    48061 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgcgatccgc
    48121 gacgcatgtt gcaaaaataa tgcgtaatta tgccgttacg tttgccaaac gttcctgcac
    48181 aatggcgatg aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa
    48241 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgtccgg tattaaaatt
    48301 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    48361 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc
    48421 tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc
    48481 atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    48541 aaccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    48601 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    48661 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt
    48721 tggcagtaaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    48781 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    48841 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    48901 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    48961 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    49021 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    49081 attaactatt tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag
    49141 gtcaatacat ctgatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt
    49201 taccaactga ttgataccca aaatggtcgg gtcactccgc gtatttatac cgacccagat
    49261 atttaccaac tggagcttga acgtattttc ggccgttgct ggctatttct cgcccacgaa
    49321 agccagatcc caaaacctgg tgatttcttt aacacctaca tgggggaaga tgcggttgtc
    49381 gtagtgcgtc agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc
    49441 atgcgtgtga gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc
    49501 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    49561 caagggttgt gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa
    49621 gggctgattt ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac
    49681 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    49741 ggcgtacaaa agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt
    49801 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    49861 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    49921 aaagatgcgc tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa
    49981 ccggatgcta atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc
    50041 gaagcagaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatatt
    50101 ttccccacgc tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc
    50161 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    50221 aaagccgctt ttgaaaacag cgccactcgt gcttttgatc ctgctggttt tctcgagcag
    50281 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    50341 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    50401 ggcattacta actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc
    50461 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    50521 gaggtgatga aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc
    50581 tctttcacga agccagctta ctggacgact ggaaatttcg tgactggctg gcgcagctcg
    50641 acgaagagat tcgttacacc atgcgcacca cagttaacgc gcaaacacgc gaccgccgca
    50701 aaggcgtcca gccaccgaca acctggattt ttaatgacac caaagaccag ctggagcggc
    50761 gaatcgcccg tctggaaacg ggcatggcct gggcagaaga gccgccgtca cgcacccgtc
    50821 acttaatcag caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgtgcggg
    50881 taaattatct gctttatcgg gcacaaaaag agcgcgatga aacattctat gttggaacgc
    50941 gtttcgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    51001 tcctggatca agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat
    51061 ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc
    51121 cgtcatcgcc ctgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca
    51181 tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    51241 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    51301 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    51361 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    51421 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg
    51481 tcggcggcaa aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa
    51541 ggtaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    51601 ggcaagctgg attgttttat cggcaatgca ggcatctggg atcacaatgc ctcactggtt
    51661 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc
    51721 ggttacctgc tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg
    51781 attttcacac tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct gtacaccgcc
    51841 agtaaacatg ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg
    51901 cgggtgaatg gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    51961 gggcaaagtg aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    52021 ccgctgcaat ttttcccgca accggcggat tttacgggtc cgtatgtgat gttggcatcg
    52081 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt
    52141 cgcggcattc gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa
    52201 accatcatta ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    52261 caagggttca ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga
    52321 cctccactct cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc
    52381 gctaactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg
    52441 ggtcgcgaca cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt
    52501 tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    52561 tgctttaccc tacgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    52621 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    52681 cagcgcagat gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca
    52741 ccaccgcccg tgcaacgcta tcttttacag cgccaccagc aggctggtgt gcgcattctg
    52801 ctcaataatg ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt
    52861 ggggaaacgc ttcaggctga tgtggtgatt tacggtattg gtatcagcgc caacgagcaa
    52921 ctggctcgcg aggccaacct tgatactgcc aatggcattg tcattgatga ggcttgccgc
    52981 acctgcgatc ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt
    53041 gcactacacc gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc
    53101 gcaatgttag ggctaccgct accgctactg ccgccgccgt ggttctggag cgatcagtac
    53161 agtgataact tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac
    53221 ccggaaactc agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcagtc
    53281 acgctgaatc aggggcgtga gattcgccca attcgcaaat ggatccagag cggcaaaacg
    53341 tttgatgcga aactgctgat agatgagaac atcgcgctta aatcactgta accaggataa
    53401 ttagcgaata tctcaatgcc tggggcgtgg cgaggtgcaa gagtgtgtat tacgtttaaa
    53461 tcacataatc ttgcaaaggg attggttatg aacacactac gttattttga ttttggagct
    53521 gcccgccccg ttttgttatt aattgcccgt atcgccgtgg tcttaatttt cattattttt
    53581 ggttttccca aaatgatggg ctttgacggt acggtccaat atatggcctc gttgggcgcg
    53641 ccgatgccga tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta
    53701 atcgtgcttg gctttttcac ccgtccgctg gcggtgctgt ttattttcta cacgctgggt
    53761 acggcggtga ttggtcacca ttactgggat atgaccggcg atgcggttgg gccaaatatg
    53821 attaatttct ggaagaatgt cagtatcgct ggcgcattcc tgctgttggc aattaccgga
    53881 ccgggggcga t
//
LOCUS       DAHBHC010000026        53150 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHBHC010000026 DAHBHC010000000
VERSION     DAHBHC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.026"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(58..1239)
                     /gene="cynX"
                     /locus_tag="M2A35_001944"
     CDS             complement(58..1239)
                     /gene="cynX"
                     /locus_tag="M2A35_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HCB7198253.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(1245..1715)
                     /gene="cynS"
                     /locus_tag="M2A35_001945"
     CDS             complement(1245..1715)
                     /gene="cynS"
                     /locus_tag="M2A35_001945"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HCB7198254.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(1746..2405)
                     /gene="cynT"
                     /locus_tag="M2A35_001946"
     CDS             complement(1746..2405)
                     /gene="cynT"
                     /locus_tag="M2A35_001946"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HCB7198255.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            2515..3414
                     /gene="cynR"
                     /locus_tag="M2A35_001947"
     CDS             2515..3414
                     /gene="cynR"
                     /locus_tag="M2A35_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HCB7198256.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALHELGAGKRAIHDVAALTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(3454..4737)
                     /gene="codA"
                     /locus_tag="M2A35_001948"
     CDS             complement(3454..4737)
                     /gene="codA"
                     /locus_tag="M2A35_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HCB7198257.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(4727..5986)
                     /gene="codB"
                     /locus_tag="M2A35_001949"
     CDS             complement(4727..5986)
                     /gene="codB"
                     /locus_tag="M2A35_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCB7198258.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(6229..8115)
                     /gene="prpE"
                     /locus_tag="M2A35_001950"
     CDS             complement(6229..8115)
                     /gene="prpE"
                     /locus_tag="M2A35_001950"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HCB7198259.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(8155..9606)
                     /gene="prpD"
                     /locus_tag="M2A35_001951"
     CDS             complement(8155..9606)
                     /gene="prpD"
                     /locus_tag="M2A35_001951"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HCB7198260.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(9640..10809)
                     /gene="prpC"
                     /locus_tag="M2A35_001952"
     CDS             complement(9640..10809)
                     /gene="prpC"
                     /locus_tag="M2A35_001952"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HCB7198261.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVSGWVAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(10854..11744)
                     /gene="prpB"
                     /locus_tag="M2A35_001953"
     CDS             complement(10854..11744)
                     /gene="prpB"
                     /locus_tag="M2A35_001953"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HCB7198262.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            11983..13569
                     /gene="prpR"
                     /locus_tag="M2A35_001954"
     CDS             11983..13569
                     /gene="prpR"
                     /locus_tag="M2A35_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HCB7198263.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(13667..13942)
                     /locus_tag="M2A35_001955"
     CDS             complement(13667..13942)
                     /locus_tag="M2A35_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7198264.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            14089..14760
                     /locus_tag="M2A35_001956"
     CDS             14089..14760
                     /locus_tag="M2A35_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCB7198265.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(14777..15022)
                     /locus_tag="M2A35_001957"
     CDS             complement(14777..15022)
                     /locus_tag="M2A35_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198266.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(15435..16250)
                     /locus_tag="M2A35_001958"
     CDS             complement(15435..16250)
                     /locus_tag="M2A35_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198267.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(16493..17542)
                     /gene="yahK"
                     /locus_tag="M2A35_001959"
     CDS             complement(16493..17542)
                     /gene="yahK"
                     /locus_tag="M2A35_001959"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCB7198268.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(17629..18585)
                     /locus_tag="M2A35_001960"
     CDS             complement(17629..18585)
                     /locus_tag="M2A35_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7198269.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(18582..19553)
                     /locus_tag="M2A35_001961"
     CDS             complement(18582..19553)
                     /locus_tag="M2A35_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7198270.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(19546..21030)
                     /locus_tag="M2A35_001962"
     CDS             complement(19546..21030)
                     /locus_tag="M2A35_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCB7198271.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(21079..22062)
                     /locus_tag="M2A35_001963"
     CDS             complement(21079..22062)
                     /locus_tag="M2A35_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7198272.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(22317..22727)
                     /locus_tag="M2A35_001964"
     CDS             complement(22317..22727)
                     /locus_tag="M2A35_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCB7198273.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(23026..24408)
                     /locus_tag="M2A35_001965"
     CDS             complement(23026..24408)
                     /locus_tag="M2A35_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCB7198274.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(24418..25368)
                     /locus_tag="M2A35_001966"
     CDS             complement(24418..25368)
                     /locus_tag="M2A35_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCB7198275.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(25511..26929)
                     /locus_tag="M2A35_001967"
     CDS             complement(25511..26929)
                     /locus_tag="M2A35_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCB7198276.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(26929..28476)
                     /gene="fdrA"
                     /locus_tag="M2A35_001968"
     CDS             complement(26929..28476)
                     /gene="fdrA"
                     /locus_tag="M2A35_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCB7198277.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(28466..29329)
                     /locus_tag="M2A35_001969"
     CDS             complement(28466..29329)
                     /locus_tag="M2A35_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCB7198278.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(29369..29974)
                     /locus_tag="M2A35_001970"
     CDS             complement(29369..29974)
                     /locus_tag="M2A35_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCB7198279.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            30232..30729
                     /locus_tag="M2A35_001971"
     CDS             30232..30729
                     /locus_tag="M2A35_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCB7198280.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            30821..31753
                     /locus_tag="M2A35_001972"
     CDS             30821..31753
                     /locus_tag="M2A35_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7198281.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(31795..32883)
                     /locus_tag="M2A35_001973"
     CDS             complement(31795..32883)
                     /locus_tag="M2A35_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCB7198282.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(33025..37008)
                     /gene="ehaA"
                     /locus_tag="M2A35_001974"
                     /pseudo
     CDS             complement(33025..37008)
                     /gene="ehaA"
                     /locus_tag="M2A35_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
     gene            complement(37581..39614)
                     /gene="betT"
                     /locus_tag="M2A35_001975"
     CDS             complement(37581..39614)
                     /gene="betT"
                     /locus_tag="M2A35_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCB7198283.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            39743..40330
                     /gene="betI"
                     /locus_tag="M2A35_001976"
     CDS             39743..40330
                     /gene="betI"
                     /locus_tag="M2A35_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCB7198284.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            40344..41816
                     /gene="betB"
                     /locus_tag="M2A35_001977"
     CDS             40344..41816
                     /gene="betB"
                     /locus_tag="M2A35_001977"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCB7198285.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            41830..43518
                     /gene="betA"
                     /locus_tag="M2A35_001978"
     CDS             41830..43518
                     /gene="betA"
                     /locus_tag="M2A35_001978"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCB7198286.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKKMSRDVN"
     gene            43715..44365
                     /locus_tag="M2A35_001979"
                     /pseudo
     CDS             43715..44365
                     /locus_tag="M2A35_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44626..45321)
                     /locus_tag="M2A35_001980"
     CDS             complement(44626..45321)
                     /locus_tag="M2A35_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCB7198287.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(45314..46741)
                     /locus_tag="M2A35_001981"
     CDS             complement(45314..46741)
                     /locus_tag="M2A35_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCB7198288.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEQ
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEANGESFRSWFKKHLAQEKKNG"
     gene            complement(46752..47471)
                     /locus_tag="M2A35_001982"
     CDS             complement(46752..47471)
                     /locus_tag="M2A35_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCB7198289.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(47998..48852)
                     /gene="rclR"
                     /locus_tag="M2A35_001983"
     CDS             complement(47998..48852)
                     /gene="rclR"
                     /locus_tag="M2A35_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCB7198290.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            49078..50403
                     /locus_tag="M2A35_001984"
     CDS             49078..50403
                     /locus_tag="M2A35_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCB7198291.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILHDQIFTHPSMSESLNDLFSLVK"
     gene            50512..50748
                     /locus_tag="M2A35_001985"
     CDS             50512..50748
                     /locus_tag="M2A35_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7198292.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            50760..51353
                     /gene="rclC"
                     /locus_tag="M2A35_001986"
     CDS             50760..51353
                     /gene="rclC"
                     /locus_tag="M2A35_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCB7198293.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(51513..52382)
                     /locus_tag="M2A35_001987"
     CDS             complement(51513..52382)
                     /locus_tag="M2A35_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCB7198294.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            52631..>53150
                     /locus_tag="M2A35_001988"
     CDS             52631..>53150
                     /locus_tag="M2A35_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7198295.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFK"
BASE COUNT        13200 a        14135 c        12825 g        12990 t
ORIGIN      
        1 tcagtgatct acgtaggccg catccggcgt gaacaaagcg cagaaacaag agtcgcatca
       61 tgcctctttg acccacagct gcggaaaacg tgctggtgca aaacgcaggg ttataatcat
      121 cagcccaacg acgcacagcg catgaaatgc ccagtccatc aggtaattgc cgctgatact
      181 acgcagcacg ccagaaaacc acggggcaag cccggcgatg ataaaaccga ttccctgcat
      241 aaacgccacc agcttgccag caatagccgg ttgcacagag tgatcgagcg ccagcagcaa
      301 acagagcgga aacgcgccgc ccagacctaa cccacacacc atcgcccaca ataccggcaa
      361 ttgcagcggc aaccagataa agccgcagaa ccccaccagt tgtaacacca gcgccagcat
      421 taacagtttg cgccgatcct gatggcgagc catagcaggc atcagcaaag ctcctgcggc
      481 ttgcccaagc gtcatcaatg ccagtaagga accgctgtac tgcgcgctgg caccaatctc
      541 aatatagaaa gcgggtaacc aggcaatcag gctggcgtaa ccgccgttaa tcagaccgaa
      601 gtaaacaccc agcgtccacg cgcggggagt gaataccacg cgaaccggag tggttgttgt
      661 cttgtgggaa gaggcgacct cgcgggcgct ttgccaccac caggcaaaga gcgcaacaac
      721 ggcaggcagc gcccaccagg cgagtgtttg ataccaggat tcgctatgtt gaactaacca
      781 gggcgttatg gcggcaccaa gtccaccgcc gcccatcaga gccgcagacc acagccccat
      841 caccagtggc gtgcgctgct gaaaccgccg tttaatcacc gaaggcatca ccgcctgaat
      901 gatgccgatc cccaccccac caagcagtgc gctgctaagc agcagcgcac tttgggggta
      961 aagctcacgc atcaatgcac cgacggcaat cagcaacaga ctgatggcga cactgcgacg
     1021 ttcgctgaca tgctgatgaa gccagcttcc ggccagcgcc agcccgccca tggtgaccac
     1081 cggcagagcg gtcaacaggg cagccacgct aaagctcatt ccgctcgcct ggcgcaattg
     1141 cggtagcagt ggcccgacgg aggtgagcag tggtcgcata ttaagaccaa tcagcaccag
     1201 aaccagcagc atcgtgaacg tcttttgatg attgcgcatg gctgtcagaa cggcttggtc
     1261 ggcagatatt taccatctaa ggtaatgacc gcacgttcgc caccttccgg gtccgccact
     1321 ttcttaacgt cgagtttgaa gttaatcgcg ctgataatgc catcgccaaa cttctcatga
     1381 accaacgctt tcagggttgt gccgtacacc tgcaacattt catagaaacg atacatcgtt
     1441 gggtcggttg gaatacggtc atcaatgcag ccacgcagtg gaatcatctg caacagtaga
     1501 atggcgtctt cgtcgagatc cagcttcgcc ccgaccaggc gggcggcgtc ggcaggaagc
     1561 gcttgctgcc ccagcaaagc cgcggttacg aaggcttctg ccagaccggt accgtcggca
     1621 atctcggcaa atgagagatc ttttttagct ttgctgagca aaatggcatc ggcaagatca
     1681 agacgaatat tgcggttaat ttgtgactga atcatggtgg atctcctgat ggtttaaaaa
     1741 taaggttacg ctgcggtcgg ttggcgtagc ggtatggcac aaacgcgagg attagcggcc
     1801 agtggcacaa actggcgggt tgcgccgtca aaagctgcga tgctgccgct ttcaatgtcg
     1861 tagacccagc cgtgcagggc gatccgcccc tcttcgagcg ccaggcgcac cgatggatga
     1921 gtttgcaaat tagccaactg agcaatgacg ttttcacgta ccatcgccgc agcttttgac
     1981 ggtaaatcgg aatgcgggcg cgcctcatta acgacgcggg ctgaatcggc ataacgcagc
     2041 cagtgggaga cggcaggcat atggtccatg cactgacagc tggcaatggc ggtcatcgcg
     2101 ccacagttgg aatgaccaca aatcacaatg tcagataccc gaagcgcagc gacggcatac
     2161 tccaccgaag cagaaacgcc accgggttcc ggcccgtagg aagggacgat attgcccgcg
     2221 ttgcgaataa cgaacagatc gccaggctca cgttgcgtca ccagctcagg gaccagacgg
     2281 ctgtcggagc aggagataaa aagtgtgcgc gggctttgct gtgtcgccag ctgtttaaac
     2341 aaggcttccc gcttcggaaa tgcctcgcgc tggaatttaa ggaatccatc aataatctct
     2401 ttcaccgtta acctctgtct gtctctggaa tgagaggcag actactcgcc gattgtcata
     2461 aggtaaaagt ctcatttatg atgagttcta ttggatttac ttataggttg gcgaatgctc
     2521 tctcgacata tcaattactt tcttgccgtg gctgaacatg gcagcttcac ccgtgccgcc
     2581 agtgcgttgc acgtctccca gcctgcgctt tcccagcaga ttcgccagtt agaggagagt
     2641 ttgggcgtgc cgctgtttga ccgtagcggg cgaacgattc gtctcaccga tgcaggagaa
     2701 gtctggcgac agtacgccag ccgggcgtta catgaactgg gagcgggtaa acgggcgatt
     2761 catgacgttg ccgctctgac gcgaggatcg ctgcgtatcg ccgtcacccc cacctttacg
     2821 agctacttta tcggcccctt aatggcggat ttctatgcgc gctaccccgg catcacgctc
     2881 cagctacagg aaatgtcgca ggagaaaatc gaagatctgc tttgtcgcga cgagctggac
     2941 gttgggattg ccttcgcgcc tgtgcattcg ccggagctgg aggcaattcc tttactgaca
     3001 gaaagtttag cgttggtggt ggcgaaacat catccgctgg ctgcctgtga acaggtggcg
     3061 ttgagtcgct tgcatgatga aaaactggtc ctgctcagcg cggaatttgc caccagagag
     3121 caaattgacc actactgcga gaaagcgggg ctacatccac aggtggtcat tgaggcgaac
     3181 tcaattagcg cggttctgga gctgattcgc cgcacttccc tttccacatt gttaccagca
     3241 gcgattgcca cacaacatga cgggcttaaa gcgatttctc ttgccccgcc actactggag
     3301 agaacggcgg ttttgttgcg gcggaaaaat agctggcaga ctgccgctgc gaaggcattt
     3361 ttgcacatgg cgttggagga atgtgcggat gttggcgaga atgaatcacg gtagccactg
     3421 acacaggtca gtggctcttc aacacagagg aaattaccgt ttgtaatcga tggcttctgg
     3481 ctgctccaga tatacggtgg tttgtgccgg ttgtgtactg gcaatcacct tgccgccacg
     3541 taccgaataa cgtaccggaa cctgacggcg cagcgcatca aatccatttt cagccggcag
     3601 gataatcagg tttgcgctgt ttccggcggc aatgccgtaa tcctgcaaat tcaatgttct
     3661 ggcactgtgg tgggtgatta aattcaggcc atcgttaatc tgcccgtagc ccatcaactg
     3721 gcaaacatgc agccccatat gcagcacttg cagcatattc gccgttccca gcggatacca
     3781 cggatcgaag acatcatcgt gaccaaagca gacgttaatg ccggactcca gcatctcttt
     3841 aacgcgcgtg atgccgcgac gttttggata cgtatcgaaa cgtccttgca gatgaatatt
     3901 gaccagcggg ttggcgacaa agttaatacc ggacattttc agcaagcgga acaggcgtga
     3961 ggtatacgcc ccgttatagg agtgcattgc cgtggtgtgg ctggcggtga ctcgcgcgcc
     4021 catgccttca cggtgcgcca gggcagcaac ggtttcgaca aagcgcgact gctcgtcatc
     4081 gatctcatca cagtgaacgt cgatgagacg gtcgtatttt tgcgccaggg cgaaggtttt
     4141 atgcagcgac tccacgccgt attcacgggt aaattcaaaa tgcggaatcg cccccactac
     4201 atctgcccct aagcgtaacg cctcttccag caacgcttca ccgttgggat acgacaaaat
     4261 cccttcctga gggaaggcga caatttgcag atcaatccac ggcgcgactt cctgcttcac
     4321 ttccagcatt gctttcagcg cagttagcgt tgcatccgaa acatcgacat gggtacgcac
     4381 atgctgaatg ccgttggcaa tctgccattt cagcgtttgc caggcgcgtt gtttcacatc
     4441 gtcatgggtt aataacgctt tgcgctcggc ccagcgttca atgccttcaa acagcgtgcc
     4501 ggactgattc cagttcggtt gtccggcggt ttgcgtggtg tccaggtgaa tatgcggctc
     4561 cacaaacggc ggtataacta aaccttgttc ggcatccagg ctgttttcag ttatgggcat
     4621 cacgccggat tgcgcatcaa tggcgctgat ttttccgtcc tgcagatgaa tctgccacag
     4681 cccctctttg cctggtaacc gggcgttaat aattgtttgt aaagcgttat tcgacactgt
     4741 taacctccac atgcgtcatt gctgctgtcg ttttacgatt caaaatcggg ttaaggatca
     4801 gatagctcag cgcgccacct aataccgcgt tgaccggaac aattcccggt aaccagtggc
     4861 ctgcggcaat ccccagcgcg accgccagaa tcgccaccca attgacactc atcatacgcg
     4921 tggtcgcaaa gtgctcatag cggcgacggt tcatcagata gtcggcgatg atcacgcccc
     4981 ctactggagg aatagctgcc gaaaggaagg tcaaccagcc gacaaaattg ttatacagcc
     5041 ataatgcgca gaccgtaccg ataataccgt tgattaccga aagggttttg ctcgacatac
     5101 cggtaatgtt ggcgaaacct aaacccgacg catagagtgc gttatcgttg gtggtccaga
     5161 tattcagccc cagcaccaca atcgcaggca gcagcaggcc ctgagcaatc atcacatcag
     5221 agatatccgc catgcccagt gccgcagcgc ccgctgcacc gaaaataaac atcaacgagt
     5281 tgccgaggaa aaaggccacc atcgccacca gcaccgccag tttggcattg cgaccaaacc
     5341 ggacaaagtc agcggtgagc gtacccgcac tgataaatga ccccacaacc agcgccagcg
     5401 cgacattgaa atctaacggt tgtgcgggaa cgaccgcttt taatgcgtcc aggccgccca
     5461 tgccgttaac agccagccac acggaataac cgcccaggca ggcgatagcc ggaaccgcaa
     5521 tcaccgaaag aaccgtcagc gccgaaatgc caaaaaagac ggtgacggtc atcagtaaac
     5581 cggaaacggc aatcagcaaa ttaatatcca gcccggttgc cttacccacc ggaatggcaa
     5641 acatcgccac accgacgcca aaccagccaa cctgagttcc gcccagtagc agtgaaggca
     5701 gccatgagcc tttaacacca aacgagaagc gagcaagaag atgagtggtc aggccggttt
     5761 ttgcgccaat gtaaccgaga aatgaagtgt aaataccgag gagaagatta ccgatgagaa
     5821 ctgcgaggaa gaaatcatga tagctaagac cggttccgag agtgccgccg gtccacatac
     5881 tggcggaaaa gaaggttaat cccagcatga cgaacgtcaa tgccaatacc cctttccgcg
     5941 ccgactgcgg gactggcccc tggctaaagt tgttatcttg cgacacgaaa ttcccccttt
     6001 ttgctgtttg aaacccaaaa aatccgccgc attctattca tctgaagata aaaagcaatc
     6061 gttttcgtgg ggaaatatat tttttatatg aatgagtgca aaaaggtggg ggaaaaatta
     6121 tcggtgtaaa aacagtgggt agtgggtagg cctgataaga cgcgtcagcg tcgcatcagg
     6181 catcggcgca tggtgtcgga tgcgccacgc gacgcatccg acaatacctt actcttccat
     6241 cgcctggcgg atctgatcca atgacgcagg atcatcaatg gtcgtcagat ccccaggatc
     6301 gcgtccttcg caaattgcct ggatcgtgcg gcgcagcatt tttccggatc gcgttttcgg
     6361 caattgcgag acaaaccaga cgtgcgccgg gcgaccaaag ttgccaatct ggctgtccac
     6421 cagcgccata atcgccttct cttgcgaatg cgccacatca cgatcttcca gactatcgct
     6481 ctctttcgga atgacaaacg ccaccgccac ctgccctttc agcgcatctt tcaccccaac
     6541 caccgccact tcggcaacgc ccggatggct ggagatactc tcttcaatct cgcgcgtgcc
     6601 cagacgatgc ccggcaacgt taattacatc gtcagtgcgc ccgagaataa agtgataacc
     6661 gtcagcgtca cggatgcccc agtcaaaggt ggcgtacacc gggcgggaaa acagcgacca
     6721 gtaagtcttc acaaagcggt cgtcgtcgcc ccagatggtc tgaatacagc ccggcggcag
     6781 cggcccttcc accaccagca tccctttctc gttgacgcca cacggttcgc cggtgacttc
     6841 attaagcaac tgcacgttat agccatacat cggcacgccg gggcttccca gacgcgtcgg
     6901 cctgtcgtcc agaccgcgag caatcgccat aatcggccag ccggattcgg tctgccagta
     6961 gttgtcgatg accggcacat ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag
     7021 cggttctcca gccagataga gcacttccag cgacgagaga tcgtgtttgc gaatttcagc
     7081 ggtagggaat tttttcagca cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg
     7141 atatttctcg acgattttcc accacacgcc gcagtccggc caggtcggca atccttcgta
     7201 aacgatagtc gccatccccg ccagcagcgg cgcgtaaacg atatacgaat gccccaccac
     7261 ccagccgata tccgatgcgc aaaagaacac gctgcccgct ttgccgccaa aaatggtgtc
     7321 catcgaggtc gccagcgcca ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt
     7381 gccggtcgtg ccggaagtgt agagaatgca ggaggtttcg ttggattcca gccacgccac
     7441 cggtacccgc gcgccgatgt gttgatggcg caacgacgcg aaatcgacat cccgcccgct
     7501 gacgcgcgcc attttcgcca gcccgcgatc caccagcaaa acatggcgtg gctggtgctg
     7561 cgcctgactt atcgcatcgt cgagcaattt tttataggga atgattttgc caccgcgcgc
     7621 tccggcatca gccgagacaa tcagcaccgg tttagcgtca tcaattcgcg ccgccacgct
     7681 gtgcgaggca aaaccaccaa ataccaccga atgaatagca ccaatgcgcg cgcaggccag
     7741 cagagtaata tgcgcttcgg caatcatcgg catatacacc agcacccgat cgccgcgctg
     7801 cacgcccagt gagcgcaaca ttgaggccac cgcgttcact tcgtcatgca gctggcgaaa
     7861 ggtaaaggtg cgctcttctt ctgtttccga agagacggca atcaatgcca gtgcctctgg
     7921 ctgtttctcc agccagcggt cgatagcgtt gtggcacaaa ttggttcggc cttcacaaaa
     7981 ccaacgggca aacggcggat tgctgtggtc gagcgtttgc gtaaagggcg tctgccagtc
     8041 aatacgccgg gcctgctcgg cccagaactg ctccggttcg ttaatcgaac gctgataaaa
     8101 ttcgctaaaa gacataatgc tctcctgttg aacttacgcc ttaccgccgt ttacttaaat
     8161 gacgtacagg tcgagatact cattgaccgg catctgttcc aggcgagttc tgtcgagaga
     8221 aacctccaga atgcgctgct gttggcgagt cgggaactgg cgcgcgagat tgattttgaa
     8281 tttatcgacc agtttcggaa taccatcctg acggcggcga gcatgaccaa tggggtactc
     8341 caccaccact tcttcaaatc gtgtgccgtc ggtgaactca agggtaatgg cattggcgat
     8401 ggcgcgtttt tccgggtcgt ggtagtcagc ggtaaatgcc ggatcttcaa agcaattgat
     8461 cttctcgcgc agggcgtcga tgcgtttatc ttgcgcaacg ttgtcctcgt aatctgccgc
     8521 cgttaagcgc ccgaataaca gcgggatcgc caccatgtac tgaatgcagt ggtcgcggtc
     8581 agccgggtta ttgagcggcc cctttttgtc gatgatgcga atacaggctt cgtgggtgcg
     8641 aatggtcact ttttcgatat ccgccgccgt cttgcctgct gcctgcatct gttcatagag
     8701 cgtcatcgcc gcttcaactg ccgtctggga gtggaactcc gccgggaagg agattttgaa
     8761 cagcacattc tccatgacgt aagaaccgta cggacgctgg aagcggaacg attcaccttt
     8821 aaaggagacg tcgtagaagc cccataccgg cgcagtcagg gctgacgggt aacccatttc
     8881 gcccgttttc gccatcagtg ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca
     8941 ggatttacgc gtgccggtgt tcggcgcatg gcgataggtg cgcagcgact gaccgtccac
     9001 ccacgccagc gaaacggcgt tgagaatttc ctcgcgggtc aggccgagca tttcggcgac
     9061 cacggcggtg gaagccactt tcactaacag aacgtggtcg aggccgacgc ggttaaagga
     9121 gttttccagc gcgatgcagc cctgaatttc atgggctttg atcattgcgg tcagcacctg
     9181 tttcatggtc aacggcgctt tgccgctggc gaccgcgttg cgcgaaagcc agtccgccgt
     9241 tgccagaatg ccgccgaggt tgtcggaagg atggccccac tccgccgcca gccaggtatc
     9301 gttgaaatcg agccagcgga tcatcgcgcc gatgttaaat gccgcctgga cggggtcgag
     9361 ctggaactga gttccgggga cgcgcacgcc gttgggtacg acggtgccgg gaacaattgg
     9421 ccccagcagt tttttacagg ccgggtattc gagagcttcc agaccgcagc cgagcgtgtc
     9481 gagcaggcag taatgtgcgg tgtcgtaggc cactttagag ctgatttcgt agttcatgac
     9541 gtaatcgacg atatcaacga tttcacgatc aaattccggg cggatgttgt tgatttgagc
     9601 tgacattggg tacgtttcct tattgttatt cgtagaggtt tactggcgct tatccagcgc
     9661 gacaaacggg cggtcttccg gtccaacata attggcggaa ggacggataa ttttgttgtc
     9721 ctgacgttgt tcgataatgt gcgccaccca gccgctgacg cgggcgataa caaacagtgg
     9781 tgtgaacatc tcggtgggaa cgcccatcat gttgtaggaa acagcagaga accagtcgag
     9841 attggggaac atctttttgc tctcccacat caccgtttcc aggcgatcgg cgatgttgta
     9901 catcttcagc gagccgcctt cctgcgagag ctgcttcgcc acacgtttaa tcacctggtg
     9961 gcgcgggtca gcgatggtgt aaaccggatg accaaaacca atgaccactt ctttgttttc
    10021 cacgcgcttg cggatatctg cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc
    10081 cagcgacact tcattcgccc cgccgtgttt tggcccgcgc agtgcgccaa tcgcgccaat
    10141 aatcgcggaa tacatatcag agccggtgcc cgcaatcacc cgactggtaa aggtggaggc
    10201 gttaaactcg tgttcggcgt acagcaccag cgagatatgc atcgcctttt cccagctttg
    10261 cgatggcttt tcgccgtgca gcagatgcag gaagtgaccg ccgatggagt cgtcatcggt
    10321 ttccggctgg atgcgttcgc cgttgtgact gtagtgatac caataaagga gaatcgagct
    10381 aagtgatgcc agcagtttgt cggcaatatc ccgcgcgcca gagacagtgt gcccctcttt
    10441 ttctggcagt gtgcagccga gcgcggaaac gccggtgcgc atgacatcca tcgggtgcga
    10501 cgccgccggt aaggcttcca gcacggtacg cacattagct ggtaaaccac gcagggcttt
    10561 cagtttcgtt ttgtaagcgg cgagttcgtc acgggtcggc agtttgccgt ggatcagcag
    10621 gtgcgccact tcttcaaatt cgcaatgttc cgccagatca agaatatcgt agccacggta
    10681 atgcaggtca ttgccacttt tacccacggt gcagagcgcc gtattgcccg ccggaacgcc
    10741 agaaagtgct accgattttt tcggtttaat gacatgggta ctgttttgca ggatcgttgt
    10801 gtcgctcata ttgtcctcgt catttgtagg gtcgagatat ttttcgttgt gttttatttc
    10861 acctggctac gggcaaacag gtcgtcgagc ttctcttcgt actggtagta gttgatgctt
    10921 tcgtacagct cgttgcgggt ttgcatggta tcgatgacgc ttttctgcgt gccttcctga
    10981 cgcaggacgt tgtagacatg ttcagcggcg cggttcatgg cgcggaacgc tgaaagcggg
    11041 tacagcgcca ttgcgacatg ggcgctgcgt aattcgtcgg tggtaaacag cggtgttgcg
    11101 ccaaattcgg taatgttggc gaggattggc acctgcaccg catcggcaaa ctggcggtac
    11161 atggcgagtt cggtaatcgc ctccgggaac aacatctcgg cacccgcttc aacataggcc
    11221 tgcgcacgct cgatcgccgc atccagcccc tctaccgcca gagcatcggt gcgcgccatg
    11281 atcacaaaat caggatcggt tttcgcatcc accgccgcgc ggatccgatc caccatctct
    11341 tctttcgaga cgatcgcttt attcggacga tgaccgcagc gtttcgcacc aacctgatct
    11401 tcaatatgca atcctgccgc accggcttta atcatcgatt tcacggtgcg cgccacgtta
    11461 aaggccgaag aaccaaaacc gatatccgca tccaccagca gcggcagcga acaaacgtcg
    11521 gtgatacggc gaatgtcggt cagcacatca tcaagggtag aaataccgag atcgggcagc
    11581 cccagcgaac ctgccgccac gccaccgcca gaaagataaa ttgcctgata tccggcacgc
    11641 tgcgccaaca gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg attttcttta
    11701 gtcagtgcag cgcgaaacgc tttacctgga gagtgtagag acatagccca tcctttgtta
    11761 tcaacttgtt atttgcgttg ataaagacaa agcaaggggt gtgccacaat tgccttaata
    11821 aaattaaatg tttaatttca atttgttaga gataaagtca tgaactaatc cgcaaatatg
    11881 cgtttcagtt aacgtttcag gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca
    11941 agactaaacc caatattcgg tttcttaact ttgcggtgcg ctatggcaca tccaccacgg
    12001 cttaatgacg acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt
    12061 cgcgatatca gcctcgagtt tgatcacctg gcgaacatta cccctattca gcttggcttt
    12121 gaaaaagcag tgacctacat ccgcaagaaa ctggcaaacg aacgctgtga cgccatcatc
    12181 gcggctggct ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt tattttgatt
    12241 aaaccgagcg gctacgatgt gttacaggca ctggcaaaag ccggaaaact cacctcttct
    12301 atcggcgttg tcacctatca ggaaaccatt ccagcgctgg tggcgtttca aaaaaccttt
    12361 aatttgcgcc tcgatcaacg tagctacatt accgaagaag acgcacgcgg gcagattaac
    12421 gagctaaaag ctaacggcac cgaagcggtg gtcggcgcgg ggctgattac cgatctggca
    12481 gaagaagccg gaatgaccgg aatttttatc tattccgccg ccaccgtgcg ccaggcgttc
    12541 agcgatgcgc tggatatgac gcgcatgtcg ttacgccata acactcacga tgccacccgc
    12601 aacgccctga gaactcgtta cgtgctgggc gatatgctcg gtcaatcacc acagatggaa
    12661 caagtacggc agactatttt gctgtatgcc cgctccagtg cagcggtgtt gattgagggg
    12721 gaaacgggga cgggcaaaga gctggcggcc caggcgattc atcgggaata ttttgcccgc
    12781 cacgatgcgc gacagggcaa aaagtcgcat ccgtttgttg cagtcaactg cggggcgatt
    12841 gccgaatcgc tgctggaagc agaactgttt ggctatgagg aaggggcgtt taccggctcg
    12901 cgacgcggcg gtcgcgccgg gctgtttgaa atcgcccacg gaggtacgct gtttctcgat
    12961 gagattggcg aaatgccgct gccgttgcag acccggctgc tgcgggtgct ggaagaaaaa
    13021 gaggtcaccc gcgtcggcgg gcatcagcct gttccggtgg atgtgcgggt cattagcgcc
    13081 actcactgca atctggaaga agatatgcgg caagggcagt ttcgccgtga cctgttttat
    13141 cggctgagta ttttgcgtct gcaattgcca ccactgcgcg agcgggtggc ggatattctg
    13201 ccactggcgg aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc
    13261 gcattacgcc agggattaca ggcaagcgaa accgtgctgg tgcactacga ctggccgggc
    13321 aatattcgtg aactgcgcaa tatgatggag cgactggcgc tatttttaag tgtggaaccg
    13381 acgccggatt taacgccgca atttttgcag ctgctactgc cggaactggc gcgcgagtcg
    13441 gcgaaaactc ccgctccacg cttactgaca ccacaacagg cactggagaa atttaatggc
    13501 gataaaacag cagcggcgaa ttatttaggc atcagccgga cgacgttctg gcggcggctg
    13561 aaaagctgaa ctgccgaatg cagcgcgaac gctttatccg gcataaattt caatagatga
    13621 tcaccgataa acttacgcat cgggcatgtt tacatagggt tcagaattac ttcttcttat
    13681 aaatatttgc cgtgccgtgg atcttattgt cagtttgacc ggaggtcagt accaacacat
    13741 cagccccttt ttcatccgct tttttgatca aatcttcttt cgcgtctgca gtcgacattt
    13801 cattgctggt tgaaatatca cctatttttt catactgcga ttcaactttt tcaaattccg
    13861 ctttggtcat caattcagcg gcatagacat tggtaacggc taacgctaac gcaccgacta
    13921 acattttaga gattattttc atattacggc tccagagttg ttatcacact atcacccacc
    13981 cacattattg ggttgattaa taattagttc agtcagggca taaacgccag taaccggaaa
    14041 taagacactt tgtgatctct cttgcacccg attatttttg gtagaaatat gatgaagtta
    14101 cttcacttat ttatggatga aatcactatg gatcctttgc acgccgttta cctgaccgta
    14161 ggactgttcg tgattacttt ttttaatccg ggagccaatc tctttgtggt agtacaaacc
    14221 agcctggctt ccggtcgacg cgcaggggtg ctgaccgggc tgggcgtggc gctgggcgat
    14281 gcattttatt ccgggttggg tttgtttggt cttgcaacgc taattacgca gtgtgaggag
    14341 attttttcgc ttatcagaat cgtcggcggc gcttatctct tatggtttgc gtggtgcagc
    14401 atgcgccgcc agtcaacacc gcaaatgagc acactacaac aaccgattag cgccccctgg
    14461 tatgtctttt ttcgccgtgg attaattacc gatctctcta acccacaaac cgttttattt
    14521 tttattagta ttttctcagt aacattaaat gccgaaacac caacatgggc acgtttaatg
    14581 gcctgggcgg ggattgtgct cgcatcagtt atctggcgag tctttcttag tcaggcgttt
    14641 tctttgcccg ctgtgcgtcg tgcttatggg cgtatgcaac gcgttgccag tcgggttatt
    14701 ggtgcaatta ttggtgtatt cgcgctacgc ctgatttacg aaggggtgac gcagcggtga
    14761 gcagataatt tactctctac gtaatcaacc tgatttgccg aatcccggcc atcggcgatg
    14821 atagcccccg taatgcgtgg tccttatttc cgataagttt acttgagtct ttagcttccc
    14881 atttcagccc actctcccct gtgtgggctt ttttcattgg tctgacccgt cctgaatagc
    14941 caaacaatgg caatgttcca ctttgcaggg atgataataa ggggatctga ttcagaagag
    15001 ttttgaaaag ttggaccgcc atcgaggcct cgaaccccgt accatacaac aatcctggtt
    15061 gtcgtctgct cttcctgatg agctaatggc ggtttttgat atgagcctta cagcatattt
    15121 tctgtcaggc ttgagtgcga tgcgtaagat acagaaaaga ccccacacaa acatgatgga
    15181 attatgattt ttttcatatg aatgtcaacc cacacattca caaaacatct gcatgagatc
    15241 attttttata caccgcatgg ctgcgaaacc actactggtg cttttttaag cgaagtgtcg
    15301 ccgatcacaa caaaatatcg gcgaaaaaag agatggaatg ccagttggca tacagatata
    15361 cagatgaaat gattacctgc attgcgattc actctcaggt ggaataacaa cttgcaggtg
    15421 gaaccctttc tgaactaaag agtgtagcgg attttaacgt gcttatttac aggataatat
    15481 ctgtcgccta attcctggac tctattagat atccattgat ttaatcgcgc atcatctctg
    15541 tatgttttta cgtccagaac agagagcaat aacatcacca gtgattcagg aaaaacaggg
    15601 tagatatttt ttaagtgtaa taataatcca tgcgcaagcg cctgcttaat gggcgcgtct
    15661 gtctccggaa tagacgacat ttcgcgaagc catttttcca catgcataca ctctttccct
    15721 ttagggaaaa ttctcagcaa aggcatatgg tgttcaagaa gaatacattt gcaatctgtt
    15781 cgcactgaat cgataaactg cactaatttc ttaagcccgg taaagcgtcc agcctgcacg
    15841 tttgctttaa tatcaagaaa cgcatcatcc tggtcatata cccaaactgt ccctttttga
    15901 ttttcaagta tgcgaaaaac aaacttaacc gggtgatcag ggtaatccat ccagtcataa
    15961 aatccaggga aatcattaaa ctgcacattt atggtgagtg cgttatcgtc tgaccactca
    16021 cagtctcgta cggcagccat gagatgaatg agtttgtcgt catcgactaa atcatcacga
    16081 caatcataag gataatcata atattgttta atcaaaacct tgaatatatc gacacaggaa
    16141 accagggagc ttttgatatc ctctggtaac tgattgtttt taaccgtatt aagtatgctt
    16201 tgttgcgtat atggcatcaa attattgtgc ggagctttta atgatatcat cgcgatctct
    16261 cttaaataaa aataaacgtc gcagataata aagctctgat taaaccaggc actatcagaa
    16321 atcgctcata actaacgtgt gaagtattgt gtactggagg gagttaattt aataatttga
    16381 atttacgtta taggttttac tgctactttg atattatttc gctctcctta actaaaaagt
    16441 taagtggaga gcgaagttaa tatgttaaac cacagcgtat ttattaattt tttcagtctg
    16501 ttagtgtgcg attatcgata acaaaacgat atttcacatc accgcgcagc attcgctcat
    16561 aggcttcatt aatttgatcg gcccgaatca tctctatatc agccacaatg ccatgttcgg
    16621 cgcaaaaatc gagcatctcc tgagtttctg gaatgccgcc aatcatagaa ccggctatcg
    16681 cacggcgttt catgatcagg ttgaaaactt ccggcgattt atgcggtgtc gcaggcgcac
    16741 caaccagcgt catggtgcca tcacgcttca gcaaggtggt aaaatcgtcg agattatgtg
    16801 gcgcagctac tgtattcaaa atgaaatcga aactcttcag atgagccgcc atctcatcgg
    16861 cattgcgtga gttaacaact tcatcggccc ccagggcttt tgccgcttcg cgttttgcct
    16921 cagaagtggt aaatgccacc acatgtgccc ccatcgcgtg ggccagctta atccccatat
    16981 gtcccagacc gccgatgccg accacgccca cttttttacc cggcccggcc tgccagtgac
    17041 gtagcggcga atacgtggtg atccctgcac acaacaaagg agccaccgcc gccagctgct
    17101 cttgcgggtg acgaatacgc agaacatatc gctcatgaac gacgatctgt tgtgagtagc
    17161 cgcccagagt atggcccggt tcgtccggcg tcggcgagtt ataggtgccg gtcatgtgat
    17221 cacagtagtt ttccaacccg tcttcacact cttcgcaatg tttacaactg tcgacaatgc
    17281 agccgacacc gaccagatcg cccggcgcat atttttctac ctgatcacca acggctacca
    17341 cacgccccac aatttcatga cccggcacgc aggggtaaac cgtccccgcc cactcggaac
    17401 ggacctggtg gagatcggaa tggcaaacgc cacagtaagc gatttcgatt ttgacatcat
    17461 tcggtcccgg ttcacgccgg gtgatatcca tcggttcaag tggttgttta gcggaatatg
    17521 caccaacagc tttgatcttc attgtgttta ctcctgatta gctatgtgta tttggcgacg
    17581 cctgatatgt catcgcggca aaacgcgtcc attgaatata gccaatattt aaaccacccg
    17641 atccagcccc aacatgccgc gagcctgaat gaaacagagc agcaacgttc cccataaggc
    17701 catcgtcagg taggggctta taccgagaat attgaagccg ctttcaataa tttgcagaag
    17761 aaacagcgcc agcaccatac caattatccg cccgctgccg ccgtccggat taaccccgcc
    17821 gagtactgcc gcgaggattg acaccagcaa gtacgattcg ccataagagg ccttagccga
    17881 gttgagtttc gacatcatca aaaatgccgc taccgcacaa agcaaggcgg aaatgacata
    17941 aacccaaatc agcacccgac gagtgttaat gccggagtaa agtgttgctc gttcgttaga
    18001 cccaatcagg taaatggctt taccgagcgg cgttttctcc agcaatatcc atagccccag
    18061 tgccactgcg gcaaacaacc acatcggtag cggaataccg aaccattgcg catgattcaa
    18121 ccacagcacc catgagggat aattggcaat ggcgctacca cctgtcacca gaatatttat
    18181 ccctttgagc aacgtcatca tcccaagcgt tgcaagaatt ggcgacactc gaataccagc
    18241 aatcaatacg ccgttacata aaccgattat caccgccgcg ccagcgcccg ccagtaacgt
    18301 agcgacaacc gtagcaatac ccggtggata ctgagtcgcc acccacgcca tcaccagtga
    18361 acaggcgtta gcagtggcta taattgacaa gttaatgcca ccgcaaagca tggtcaccgc
    18421 catagccaac gccagaatgc ccagcacggg catttgcgaa gcgacggact ggaaattact
    18481 tatcgaccag aaaatctccg gcatagtaat gctgaacgcg gcaacagtta gcaccagcag
    18541 gccgatcaag taaaactcaa cgttattacg ccacgatttt ttcatagcag actccggttc
    18601 tgatgctgac tccacgccgt ggcactaatg ctggcaacga tgatgatgcc ggtgatcaat
    18661 gtttgccagt aagacgagac tcccaataaa tttagcccgt tttgcatcac tgccaacaac
    18721 accacgccga gcaaagtacc ggttaacgtg ccgcgcccgc cgagcaaact agtgccgcca
    18781 agcaccaccg cagccagtac tgtcagctca taacccagaa gagaatcggg ggcgacagtc
    18841 atcaccgtcc acgactgcac tacacccgca gcgccagaca tcaatcccat atagccatag
    18901 acgaaaagtt gcagtttcag cacgctaaag ccgatgcgac tggctgattc gcggttcccg
    18961 ccaagggcat aaattttgcg ccctacactg gtgaaattca tgataaacgc tgttagcagc
    19021 accaccgtaa tcatggcgat cagcggcaga ccaaggccat agtcatagcc atcagcatcg
    19081 gtgtacttga acaacataac cccctgctca aaccacggcg gaaagtcgta cagccacaca
    19141 cctttactca accataacag caggccatag aaaatattca gcgtcgagat agtgatgatg
    19201 atagaaggca cccgtaggcg attaaccagt aaggcgttaa ttaagccaag taaaatgccg
    19261 atgcagcccg ccagtgcaaa cgcgacagcg aagttgccac caattttttg cagcaacagc
    19321 accatgccgt attgcgcaat gatggtcatt gctggaaacg aaatatcaat tccaccggaa
    19381 atcaacacca caaacaaacc acaggcgaga atagtcaaca tggcatagtt attggcgaga
    19441 tcgtacaaat taccgaatgt cagaaactcg tcgctgcgcc aggcaaggcc aacaaacagc
    19501 accacaatca gtaatgccag ccagaattca tggcgttttt tcagttcagc catttaccac
    19561 ctccgcaatc ctggcctgag aactgctgtc aggcaggaag ctatgggtga tctgaccttt
    19621 ttgcattacc aaaatccggt ggctttgata ccacgcttct tcaatttcat cgcagatcat
    19681 caataccgca atgccatgag ccgccagatc gctgataatg ccgtatattc ctgctttgtt
    19741 ggcgatatca acaccgactg tcgggctatc gagaatcaac agtcgtggcc caatcgccag
    19801 ccatttggcg atcgatactc gctgcgcgtt accaccagaa agcgtattca ccggaagatg
    19861 aggatcagag actttgatgg tcaatgcctt gattagcctg tccaccaggt cacgaacttt
    19921 agtttcactc agaaagccga agcgatcttt taccttgtga aaaacagtgc taatgatgtt
    19981 gtcttcaata gattgcgcca tcaccaaacc gcgcgacatg cgatcttccg aaacataacc
    20041 aataccgtgg cggatagcgt cctggttact atgcagtgta accggctggc cgttgatgag
    20101 gatctcgcca gcatccggtc gggtcatacc aaacagactc agacataatt ccgtgcgccc
    20161 cgcaccaagc aggccaacaa tcgataccac ttcgcccgct tccacccgca agttaatatt
    20221 tagatattcc ccatgacgac ttaaattacg cacctccagc accggggtgc tggattttcc
    20281 ctgccacaac tctcgcacct gatattcaaa ctcctggccg gtcatcagga aaccgagttg
    20341 ttttgtggtc atctctgcag ccggaaaagt cccgaccagc tcaccatctt tcagcactga
    20401 aatacggtcg gaaacttcca tcacttcttc cagacgatga ctgacgaaga ccacgcagat
    20461 cccgcgttca cgcaattgat gcaccacctg taataaccct tgcacttcct gatgggtcag
    20521 cgaagcggtt ggttcatcca tcacgatcaa ccgtgcgtcc tgcgccagtg cacggcaaat
    20581 agccaccagc tgacagcggg caattgaaag ctcagaaacg agtgtatcca gtggcaatgt
    20641 gacgttaata ctggtcattg ccgcctgcgc cacctcacgc agacgacggc ggttaacaaa
    20701 cagaccgtgg tgatagtgat tcacggcgat attctcccag acactcaggt taggaaataa
    20761 agagaggtcc tgataaatca cctgaatccc ctgcgccacc gaagcggcgg gtgtcagctt
    20821 tgaccagttt ttcccaccaa gcgtaatttc cgctccctca tccgggcgat aaacaccgga
    20881 cataatttta atcaacgtcg atttaccaca gccgttttgc ccggcaaggc agtgcacttc
    20941 acccgacata aagtcgagag aaacatcgcg cagcgctcgc gtggcatgaa acgttttgtt
    21001 gatatgacga agggaaagga aagtttccat aacatcccct ggcaataaga atcggggctt
    21061 tcgccccgaa gaaatggatt aataaagtga gtcgatattg tctttgttga ccagtaatac
    21121 cttatggaaa cggataattc gcttatccat atctacgtcg gccttaccca gattctgcat
    21181 ttctaatcca ggtttgatct cttcaccctt cagcaacgtg ctggcgacgg ctgcgagtgc
    21241 gtaacctgca gaggccggat cgtaagtaat cccttcggtg atatcaccac ttttaatcag
    21301 tgatgccgcc tgtgaaggga tcatcatgcc atagactgcg actttatttt tcgcccgttt
    21361 ctctttcacc gcacgtcccg cgccaatcgg accgtttgaa ccaaaggaga caaccgcttt
    21421 caagtcagga taggttttca tcaggtccag tgtggtacga cgtgagtcat ccacactctc
    21481 ggcaaccggc atgcggcggg taacttcatg catatccggg taatgctctt tctggtattt
    21541 caccagcaag tcagcccata agttatgctg cggcacggtc aaactaccca cgtaaatcac
    21601 atagccgccc ttgcccccca tgcgtttcgc catatgctca acatattcag cggcaaattt
    21661 ttcgttatca atgatttcaa tatcccagtt agcacttggc tgaccggggg attcgttggt
    21721 cagaaccaca attccggcat ctcgcgcttt tttgaatacc ggttccagca cgttggcatc
    21781 gtttggcacg atagtaattg cattaacctt acgggcgatt aaatcctcaa taattttaac
    21841 ttgttgcgga gcatcagtac ttgaaggccc cacctgtgag gcattaacac caaaggcttt
    21901 acccgcctca accacacctt cgcccatgcg attaaaccac ggcataccat cgactttaga
    21961 aatattcacc acgacttttt ccgctgcctg gagcggcgca gaaattagcg cagcgcctaa
    22021 taacagcgaa gacaccatat tgataacaaa acgtttattc atcatttcag cctcattgca
    22081 gggtagtcag gaagtgttcg cccaaattag cgcgctggag agaataagaa ttttcgaata
    22141 tttttatgca gaataaacag ccctatttca ggcagctatt aaaaatatgg ttataaatat
    22201 ggacaaaacg ggcagcaaca ataagtgtga cattcatcac gtaaaaaaat agcaaaataa
    22261 acaaaattag caatatttta tgtgtgtgag tttatttcgc gtagataaat agttatctac
    22321 acaacgtcaa taagggcata ttcttcaaat ataacgtcca tttctggtga atagcttcct
    22381 tctgcctgga aaaaacgacg gtgttcatta cgccaatatt caagactgag gtcaccttca
    22441 ccttccttac gggcaagctc cacggtgaca tcagaaaagc gcagtaaatg caggccaatc
    22501 actctgataa cacatacagg ctcaccacgg ctattttcta caatcttata ctcaccaatc
    22561 atcggaaatg cgttgtcttc aatacatccc gccagcgaac cacaggaagc tgttttaaca
    22621 ccagtcgcaa tcaacgttgc gaggtgatcg gccaacgctg ctgaatcgcc aaaagtacaa
    22681 aaggaagcat ggggatattt ttgtgagaat ttttctttta tggtcatggc aatatttttg
    22741 cgattacgga aaaacaaatc acatcttaat tactgttggt aaccatcagc aagagtatca
    22801 ttggtttttc gatactgaat cttttttatc cgaagcaggt cacatttatg gcctgataaa
    22861 tacctttgtt aaaactcact tttgtgtatt tctctcaaat aatcccctgg aaattatgcc
    22921 gcgagattat aaagttcagg atcattcatt agcaaaccat tcccgcccca ctccctgggg
    22981 cttaaatctc ttacaattca ccgttgcagg cgtttcaaat gcgcatcaac ctgccacact
    23041 cccccacacc agttgccctt tatggaacgt tgcggttctc ggcgagatac gcgccaccgc
    23101 ctcggcggaa caggaggcgt ccaccagcac aaagctggca tcatcctgcg cttttggcca
    23161 tacacgctcg cctttttcat tcagcggcaa tacatcgcca gtggctagaa acagcgaacg
    23221 ggagaggttc tgttcgttag gacgaatata gagctgcgcg tagagattgg ctttttccag
    23281 catgtcgccc agaccataag gcgaccagtg gtcgataacg ctgtcagtgc cagtcatcac
    23341 ttttacgcct ttgtcgtgca actgtttgag cggcatatgc agcgtgccaa tcggcaccgt
    23401 cgaggcgata gaaatttgct gcgccgccat ccgattcgcc agcgcatcga cctgttgttc
    23461 gttgagcgta gccagcgcaa aggcgtggct gatggtcagc ttacctttca gttgcggcgt
    23521 tttctctacc gtttcaacca tataattgat ggctgccacg cccgacggag tggtttcgtg
    23581 caggtgaata tcgacacctt tgtcgtagtc cagcgcaatc tgaaacatgg tgtcgaggga
    23641 tttttccatc gcgccatcga cactggtcgg gtccagcccg ccgacgtaat gcgcacccgc
    23701 ctgcatcgct tcgcgcatta agggttccga tttcgacagc agcaaaccgt gctgcgggaa
    23761 ggcgacgatt tcacactcga agcccgcctg acgtcgcgcc agcaccgcct gcaaattctg
    23821 cagatttttc aggccggaaa ccggttcgat attgcagtgg ctgcgggcaa tggtggtgcc
    23881 tttcgactgc aatagatcga tcagtttttc tgcccgttcc tgcgtgtacg gttgcagttc
    23941 cggcagcatt ttctgctcaa gcttgatcat gtcctggata gtggtgcctg ccggacgatt
    24001 gagcgagcgc cacggcccgc cgtagaaggt tttgtcgaga tgaatatgca tgtcgcgagt
    24061 ggtgggtagc atcaacttac cgccagcgtc atagtgcggc agcgtggcgt ccggatgctg
    24121 cttgttctcg cgcagggcga caattttgcc gtcctgaatc tccacggtct ggcgcgccgt
    24181 gcgggtctgc accgccacac cgttttcgta gtcaaaaccg gtttccagca gcacgttatc
    24241 gagatagtaa tgcgggtcag tgattttcat ggtgttgttc gcttcgcagt ttagggtgcc
    24301 agctaccgcc gcatgggcga gcggcacaga ggtaccaaac agcgcggcgg cggtgaccat
    24361 cttaccgctc tggctcagaa attcacggcg gctattactt tctttcatcc tacgtcctta
    24421 cgttttaata atatgggtgc ccgtttcacc gcgcagcgcc gcaggcaggc attccggcgt
    24481 ggtgataatc acttctttgc cgccctgctc caggaacgtc aggctggcga tgatttttgg
    24541 caacatgctg ccgggcggaa aatgcccttc ctgcatatag cgagtcatgg tggcaatatc
    24601 cacccgatcc agcgcctgct gctgcggttt accaaagtgg atacagactt tttcgacgcc
    24661 ggtggtgatc accagaatgt cggcgtgaat ttcacgggcc agcagcgcgg tagagagatc
    24721 tttgtcaata accgcgtcca cgctttggta atcccccgct tcagtacgca ctaccggaat
    24781 tccaccgccg cccgcgccaa ttacgacaaa accttgttgg atcagcgcct taatggcggg
    24841 tgcttcgaca atacgtttcg gttccggcga ggcgaccacg cggcgatagc cccgtccggc
    24901 atcttcaaca aaacaccagt cagggtttgc cttttgtagt ttgtcacgct ggctttcact
    24961 gaagaatgca ccaatgggct tggtgggatg ggcaaaacct ggatcgtttt tatccacttc
    25021 cacctgagtt accacggtga cggctttttt ctcgccgtga cgcgccagcc ggttattcag
    25081 cgcctgttgg atcagatagc cgatgccgcc ctgcgtatct gccacacagt tcgccagcgg
    25141 cgttaagggc agcccttcgc gctcgtgggc aatctccgca cggcgtaaat ccagcccgac
    25201 ctgcggcccg ttaccgtggg tcagcacaat gtcgtaatcg gaagccagca tttccagcac
    25261 cgtatcggcc acggctttca ccgcctccgc ctgatgctca atcgactggc tggcgttatc
    25321 tttgataatg ctgttgccac caatggcaac gaccacaagc tctttcatgt tgttatgtcc
    25381 gctacagagg gtgattaggt tatgaccaac gccagactgc ctgatgcgct acgcttatca
    25441 ggcctacaat tctcgggcca tccgtaggcc ggataaggcg ttcacaccgc atccggcata
    25501 aacatgtaag tcagacgccg taacgttcgc accagccgag aatggctttc tcgaagcagg
    25561 cgaatggtgg atgcacaatg cccgcgccaa tcatgccgac gcccgcatct ttatgggcga
    25621 tggcggtgtt gatcaccggc agaatgccgc tgctacccac gcgggtgatg tcgattgccg
    25681 acggcacgcc cataaaaccg agcagcggaa tggtgacgtt ggggttttcg ccaagggtga
    25741 tttcgcgcat ctgacgggag aaatcgatag cctcttccac cgtgccgccc accagtgcga
    25801 cgattgcagg cgcggtcgcc atggcaaacc cgccaatacc gtaggtttct gtgatggcgc
    25861 tgtcgccgat atccagcccc gaatcttccg gcttataacc agcaaacatc gggccgatga
    25921 cctgctgcgc tgggccggta aaccactgcc ccggcaggcc gctgacccgc aggccgaact
    25981 cgacgccgtt acgcgccatg gtggtgacca cggtgctgta ttcgatgccg tgcgccgcat
    26041 ccatcgccgc tttacacatc gccatccacg tcgggccgga gaagtagtcg ctgctggcga
    26101 caaactcgaa cacttcgcgc tgttgctcga cggaatagcc tgcctgaatg atccccggcg
    26161 tcagtgcctg aatcagcagc gtcgtcccgg cgttattgcg gttatggcac tcatcgccca
    26221 tatgcagcgc ctgcgccagc attaagcgca gatcgatctc accgataatt ttcatcgcgt
    26281 cgcgcagcat tggcccctgc acatcgcgca tccagttgag gcggtcgatc acgctctgat
    26341 cgttggcacc catacgcaaa atcttcgcca tctgctcgct catattggta taagcgatgt
    26401 tgccgtaggt tttgtttttc acgatgtgca taaacatcga ggccgaggta acgcccgcca
    26461 tcgatcccac gcagtcgtgt tcgtggcacg gcgagaaggt gatctccccg gaggccgcca
    26521 gttcagtcgc ctcatcgaga tctttcgcca gcccttcgaa caccaacgcc ccggtgaccg
    26581 cgcctttcat cgcgccgcac attttttccc aggtgatcgg cggcccggcg tgaagaatgg
    26641 ttttcggcgt catgcccggc accacgttaa tcgcctgatc aaaaccaatc agcaccggat
    26701 gcgactggat aatgcgctcc agcgcctgct ggttagcggc ggcgattttg tctgccagcg
    26761 gcgaatcggc aatgttatcc agcgcctgca ccacctgcat attgccctgc cccggcggcg
    26821 tccagtcgag ttgagtcact tctacatgct gctttttcag atcatcgcta aacatggcga
    26881 taccgacgtt aataacgttc aacggttggc taaacagtga ctggctcatc aggcttcctc
    26941 ccctttgcag ataaattcac gcgccagcaa tccggtattg gtgctgctgc tcgccagaat
    27001 cactccggca tcaagcagca tctggctttg ttgttctaat gatggcgtat cgagatcggt
    27061 gcccagcaca taggcgagaa tgatcaactc gcgtccttcg gcggcggcga tcgctttcgc
    27121 ttctttgatc gcctcgatgg tggagccgac cggatcttca tgcgatccaa atccgagcac
    27181 aaaatccatc acgatcaccg ccacttctgg atcgcgcgcc tcttcgatca agcgactgat
    27241 gcggttggtg gggtcaatca ttggatgcgg cttgccgttg gtgaagtcgt catcgccaaa
    27301 gtcgaggaag gtgtgtttga tgctacggtt gatatcttgc aggcggaatt ccggatcggg
    27361 ctgaatgttg ctgtagacat cgccgtgttt ttccatcacc gcgaacatgg tttcgtcaca
    27421 cagcgtgccg ccacagaaca ggccacgaat gtacttctgc tgcggttgca ggcgagcacg
    27481 tacgtcagca atcaacggct ggttcagcgt atgcaggtcg agatgttcct gtttcacgcc
    27541 ggagagcatc accgctttca atgccgcctc tttgctgccg cgggcaaact gtagcccctg
    27601 ctcatccact ggcgtttcgc cacgaccgag gaaacagacc accaccggct tgcggcaggc
    27661 gcgcgcgcgt tccagcactt tgcgggccac cgcaggcgcg ggcggtttgg agataagcac
    27721 aatgatttca gtttgcggat cgttttccag catgccgatg gcgtcgagca tcatcaggcc
    27781 gccgattttc tcgctcaggt cgcgcccgcc ggtgccaatc agttgcgaaa cgccgccgcc
    27841 aaattcatga atgcggacgc tcagctcctg gctgccggtg ccggatgcgc caacaatacc
    27901 gatgttgccg cgacgcacgg cgttaccaaa acagagcgcc gcgccgttaa taatcgccgt
    27961 gccacagtct ggccccatca tcagcagccc tttttcgtgg gccagttgct tgagcgccaa
    28021 ttcatcttca actgagacgt tatcggaaaa cagcatcacg ttgagatcgt tttgcagcgc
    28081 ctgacgcgct tcgcgagcgg caaacagacc gttgaccgaa atcaccgcca ggttactttc
    28141 cgggatatgc tttttggcgc tggcaatagt ggcgtaacgc gcctcatgcg agccgctttg
    28201 cgctttggtg ttgaacagtt cttcaatctc caccagcaac tgttcgttgt ccgcgcccgc
    28261 tttgccgttg atgacaatca tcaggtcgcc gtttttcgcc tgttccagct ccggcgtcag
    28321 cagtcctaaa tttttcagta cacctttgtt catttcggtc gccatcgcca caaatgcctg
    28381 ctcgacgccg tcgagtttat ttgcacgcgt ggagatagac atcagcgaga cagaatcaaa
    28441 ataggtattc ggtttaatga ctattttaac tgacataatt ccctccgtag gtttggctca
    28501 gcgcacaacc atcggccatg ccatacagca tgtcgcagcc ggaactggag ccaatatgtt
    28561 taattttttc tattgcctga gttgcgttcc cagggatgtc ataaataatg ttgtgaataa
    28621 aacgatgaat attttcccgg caacgttgtt gtaatgcggc ttccagcgtt atagcactta
    28681 ataatgtggt gttatttctg ccgcgctgca taccgagata aaattcctct ttgtattttt
    28741 ccgacggatg cccgggaata aataaaataa gcgccagacc tgttaaataa tcgtcggctg
    28801 atggcgtaag gccaatccct aacccaatca tactggcgac cgctgcggcg atattttgtt
    28861 tttcgtttac tgcctgaata actgcccgtc gtttaatatg tagttcttta tttaacgcct
    28921 gataaaacgg attatcgccg cgataattaa acagggtttc attttccccc aggtgctgat
    28981 gaataatgtc gtgccactgt tgccaggcga taaggtgaaa attcacagcg gtcatttgca
    29041 aggttgggga cagccaacgc cgacaacgac tcatctctat atgaagatgt tgaccaaccg
    29101 taataccttg atcgtcaaac tgaaccttat caccatgccg gaacagatca tcaaagtgag
    29161 tgagtgccaa ccgacaactg tttggcgcat tgtcgtactc ttcacaaagc agcgtcagca
    29221 actgctggcg agccgggagt gcgatattta ccgctctggc aaagacctgt tcaacctgtc
    29281 cttgcccccg ctgcgccaga aaatccgcat ccgcagttag cgcccacaca caccgtcttt
    29341 ctggtgtgtt gatagcaagc ctcccggttt atgcacctgc ggcaatcagt aactgcgcaa
    29401 tctcttcaaa gccccgttcc cgcgccagtt ccagtggcgt tttgccatat ttatcggtca
    29461 gatgcgggct ggcaccgtgt tccagcaata actgcacaat cgcctgctgt ttaataccac
    29521 catcattaag cacaatcgct tccagtagcg gcgtccagcc tacatggttg gtctggttaa
    29581 cgttaatctc cgtatgcgcc aaaagctctt ttacaatact taaatggcct ttttcacagg
    29641 caggcgtcag gccgacaccg ccaaaacggg ttacgcaatt aagatcgggt ttagccggta
    29701 aaataattcg tagtagcgtt aaatcatcgt tcagacagct aattaaaaaa ggatttaaac
    29761 aggtatgatc ttgcttatta atatccgctc cggcatcaat taatgcctga acgcaagcat
    29821 attgctgata taaacttgcc agcgtaattg ccgttttccc ctgacgatcg caggtattaa
    29881 tatcgacacc cagcgcaagg caggttttta ctttatcgat atcaccctgt tgtgcagcca
    29941 ataaatagtc ggcaggtaga ttttttatac tcattcacat tcccctggtt tttgtggctg
    30001 ttttcgatga attgagaata gcaacgataa tctgataaaa gaacccgctt aaatttaatt
    30061 cgctgtattt cgcctttttg ttgaatgatg ttcaacaatc ttttcgcagt gtgcggtagt
    30121 gcaatatttg tttctttgac ccgataccct ttcactcaat caggttttat ttctctatag
    30181 tctgcacatg taagagtagc tttcgtccat gtctaaccag aggggaacaa gatgaacgga
    30241 ctcacggcaa cgggtgtcac cgtcggtatt tgtgcagggc tttggcagtt agtgtcttca
    30301 catgtcgggc tctctcaggg atgggaatta ttaggaacca ttggcttcgt cgccttttgc
    30361 agtttttatg ccgcaggcgg cggtaaatcg gggtttatca gaagtctggc ggttaattat
    30421 tccgggatgg tgtgggcatt tttcgccgcg ctggctgccg gttggctggc ctctgtaagt
    30481 ggactgtccg cgttctgggc gagcgtaatt actaccgtac ccttctcggc ggtagtggtc
    30541 tggcaagggc gtttctggtt actgtccttt attcccggtg gctttctcgg catgacgctg
    30601 ttttttgcca gcgggatgaa ctggacggtg acgttactgg gttttctggc gggtaactgc
    30661 gtggggataa tttcagaata tggtggacaa aaattaagcg aagcgacgac taaacgcgac
    30721 gggtattaaa agttattcaa aaatgattat ctatctttaa ccgatacctc tacagtcata
    30781 gctggagaaa ttcatagtag ataatggcga gaatggttta atgaactcaa tttttaccga
    30841 agagaatctg ttggcattta ctaccgctgc acgttttggc agcttcagta aagccgcaga
    30901 agagttgggt ttaaccactt ccgccattag ctacaccatc aagcgtatgg agacggggct
    30961 ggatgtggtg ctgttcactc gcagtacccg cagcattgag ttaacggagt ccgggcgtta
    31021 ttttttccgc aaagctaccg acctgctgaa tgatttttat gccatcaaac gcagtatcga
    31081 tactatttcg cagggcatag aagcgcgtgt gcgcatttgt attaatcagc tgctttatac
    31141 gcccaaacac accgcacgct tgttacaggt gctgaaaaag cagtttccta cctgccagat
    31201 taccgtcacc accgaagtgt ataacggcgt ctgggatgcg attattaata atcaggccaa
    31261 tatcgccatc ggcgcacccg atacactact ggacggcggc ggtattgatt acactgaaat
    31321 tggcgcgatt cgctgggctt ttgctatcgc gccggatcac ccgttagcct ttgtgccgga
    31381 acccatcgcc gaaagccaac tgcgtctcta ccctaatatt atggtggagg acaccgcgca
    31441 tacgattaat aaaaaagtgg gctggttgct gcacgggcag gagtcgattc tggtgccaga
    31501 tttcaacacc aaatgtcagt gccagatttt aggtgaagga attggttttt tgccggatta
    31561 catggtccgt gaggcgatga cgcaatccct gctggttacc cgacaaatcc ataatccgcg
    31621 ccaggattcg cgcatgttac tggcgactca gcattctgct acgggtcagg tgacgcagtg
    31681 gattaaaaaa caatttgcgc caaatggcat attaacgggg atctatcagg atttactgca
    31741 tcgggagaat taatcgctgc caacctcccg atgcgctgtg aatagcggtt tacatcaacc
    31801 acctgctttc attacccatt cagagataaa tttattaccc ggtactggcg gagagtacaa
    31861 atagccctgc aaaaagtgaa cgcctttacc gatcattaaa tccgcctgct cctgggtttc
    31921 taccccttcc gccacgatac tcagaccagg cttacgcgct agttcgacaa tattgtccac
    31981 aatatgaccg gagatttcgt cgacactcgc catttgcaca aatgacttat cgatcttaat
    32041 aaaatcgacc gggaacgcct gcaagtaacg ataggtcgca taacccgtac caaagtcatc
    32101 cagcgcaaag gtaatgttgt gctgatgaag gctgtcaaat atcgctctgg cttctggcgt
    32161 taccggaata gggttacgtt ccgttagctc gagaaccagc ttgattttat cgttacctaa
    32221 tttcttaacc aggttcagac attctttttc aaatcccgct gccaaaaaac aacccgccga
    32281 gacgttgatg ccaatatgga aattgtccgg cagcaaatgt tttaccggca tcagaatatc
    32341 cgcagtctgt ttcatcagtt ggcgggtcat tatgacaata agaccggatg actccgccag
    32401 aggaataaac tgatccggtg ggataattcc cgtttgtgga tgttcccagc ggacaagtac
    32461 ctcacagccc gtcagtaccc cagtctgcgc gcagaaaacc ggttggatcc acggtttgaa
    32521 ttcgtggttt tccaacgcca gactgatggc ttcaggcgtg acgatatggt gacaacggtt
    32581 atcagggata taaaaattat ttttatttcc cgtgccactg atgacttgcg gatgatattg
    32641 aaaaaagata tcccgccaga aggtaatatc actctggatg cctagttttt catacagctg
    32701 cttcttttga tgtgaaattg tctttgcact gcgatttctg tactgtgaga tttctgtaac
    32761 cgacattccc tccattagca gcttgagtaa aagccgttct ttttcggaca cgataccagg
    32821 caacgtcaaa ttcaccgttg ccccgaactc ttccaaaaaa acactcaaat catgcgaagc
    32881 cattgaacac accttgatta aatcttgtgc gccagctaaa tctgcgagtc ttcactctga
    32941 ctgataaagc tggctgcaag aaaatgacac agagagccga attatctccc gttcggctct
    33001 ctctcctttt actcattatt cagatcagaa ttgccactta atgccaacca ttgcagaggt
    33061 gtcattatat cccctatcgg caacctgaac gcccacattc ccccagacat tcaggttggc
    33121 atttagctgt ccttccaccc cggtttttat ctcagcaata tttcgggctc catcctgagt
    33181 gacggacacg ccatccatac tggtgctgaa atccttactg ttatgcagcc agttcacttc
    33241 tacaaacggc tggaactcgc gggatttacc gtcatccatt ttatggtggc tcttcagcca
    33301 ggtttttacg ccaagtcggg tttgaacatt gccatcaccg ttgctatgaa cgagggttcc
    33361 gttgctttcg cggtgcttat cggctttgac tcccatccag gtaacctgtg cttgcggctg
    33421 aacataccat tcattacgcg ttccctggct gccattaaat tcagctaatt tgtgtttgta
    33481 tccagcttcc agtgaagcgg taaatccttt tgatttatag gattcacttt gtaagtcatc
    33541 ccctttcact gtgttatcaa accagctgta ctgcgcccaa ctgtcgagat acgcgccatt
    33601 acgcgactcg tcatcggcat accaggtggc atagaggccg gtgctatatc cgttcacact
    33661 cgcttttgca cgataaccgg ttcgcgagga aatggttttg ctgtcgctgt tgccatatcc
    33721 cgccatgacc ccaacatgcc agctgtcgct gccgttttgg ctccactgcg cgacatcgcc
    33781 tcccagttgc agaacatagc gattgctttg ggttttcagc tggccgctgc catcacgcca
    33841 tttattatga ccaccttcat ggcgcatcca catagtggtt tgtttctgct cacccgtcac
    33901 catgtcggta tagtacgtat tacccagacg ctcatgcaga cgcgtggtga acattgtatt
    33961 cgctgctgca aggttcgcaa tgtagctacc cgcctccggt cacaggtcat tatccacatt
    34021 cagatccgga cccggcgtag gtgtcgggtt agggttcggc tctggattcg ggtttggctc
    34081 cggattcggc atcgggtctg gctccggctg cagttcagga gaatcactgc cgctggtcag
    34141 ataccagtta ccacgatttg ccccttgtcc acgcgcgaga gtgtagtcat acgcccccgc
    34201 aacgatacga ccagcctgaa caaattcgcc ctcagactta ccgtctacat ggataagttc
    34261 gataccgtta agggttttcg cacctgtacc gccagcgtta ttcaccgtaa cggcagtcgt
    34321 gccgctagta tcgccctcga caaccaattt atcggttact gagtcatcgc cattcagtac
    34381 cgtattcatt actagttgac cgttattacc gtggtagttc cccttcacca ctaacacatt
    34441 gccagcttgc tgtcctgaca ttccggtcat cagcgtaccg ttgttggtca gattgccgcc
    34501 cagggtgtaa ttcacagacg cagccgctct tttttcagcg ctattattca acgccagggt
    34561 accgttgttc tcaacgtttg cgctacccaa tccctgcgca tgacttacca ggacattagc
    34621 cgaattagca atgttcacat tcgccgtcag gttgctgtta tcgcccgcga tgtccagcgt
    34681 gccgccctga atattcagct ctccgctccc cgttaacgaa ccggttgaag tgcccccgtt
    34741 ggtaaccgtc agactgccgc catttaagct cagcagtgaa tcggcgctgc cattgagcgt
    34801 gcccacggtc tggctgtgac cattcatatc cagttcagtc tctgccgcca gacgcagttc
    34861 atgggtatta cccaacacgt tatcgttatt caacaaaagc gtcccactgc gcagatcggt
    34921 aacaccggta tagtcgttgt ctttgttaga aagcgatacg gtctgatcct tctggctgct
    34981 gaatgccagg tcaccgctgc cggtaatctt tgcgctgaga tcggcggcat tctcgctttt
    35041 accgttagag ctcagcacca gagcatcgct atcggtagcg tgtaaatcaa gctgggtcag
    35101 gccgtaaccg atatacagac cgtcattgtt taccccactc gtcagacgat agtcgtaagt
    35161 gccgacggcc gctttttgcc cattctgctg aatatcacgt tgggcactgc tggagaggat
    35221 ttgcccattt ttatcctgca attgcagatt gcccgcatcg cccagaactt ccgcaccttg
    35281 cgcatcaacc aacttaatgg tgctgttacc atcatcgact tcagtgagcg ataacgcaga
    35341 gtcaatatca cgagatactg agcttacaac gtcactgtca gaaacctgaa tcacaccctc
    35401 gccgcgcaaa tccagcgttt tactaacgtt gaccgtccct tctgtcatct gtgcacctgc
    35461 tgtcagggca ccaaaatcaa cggttccgcc ggagaatccc aaagtgctaa tggagtgagc
    35521 gcccttaccc actgttacca ggcttccctg acccgcagtg atcccgcctc tctgcagaaa
    35581 ctcagcattc ttttccaggt taaatgtcgt gttctggaac gccagtgaac cctggaacca
    35641 gtctgtataa gcaaactcat cgaaggagaa ttcgtgattc cccatgttta tttcaacaat
    35701 acctctacct tgaagatcgt tatcaaatac ccaatcttta ttggcattga tttgcagttt
    35761 gccatcaaca tccaggtccg cacggccgat gttcttgtct tcagaaataa ccgcgacagc
    35821 accagaagcg acatcaatat atccgctgaa tgcatagtta ttgccggtca gtgccactgt
    35881 cccactgtcg atacttattg taccattgcc ggaaatctta ttggcaaagg tcatgctatc
    35941 gtcagccttc aggtgtaatt tcccgcggtt gttgatctct gctgtaccta tatttttggt
    36001 gatccctaaa gcgccatttt gctcaatttt gactttacca ctaaaggcag agttatcacc
    36061 ggttaagtaa gtcgtgccat tgagcgcatt gatatagccc aggccctcgc tgccactgac
    36121 aatatccgcg gaaatagtcg cgtttttatc cgtgtggttt aaggtgattt tcttatcgga
    36181 tgtctttgcc cacacaaact caattctttc ggcatcaata atccctgcag ccttcgcagc
    36241 acttccttcc tgtgcaccta acgctaactc agagttggtg cttaaactaa ttttcggtgc
    36301 ggatatttta cctccatctg atacgactaa tgttgtatca gaacgttggt cgtaataacc
    36361 caggctaata tgttcacgag cattaacttc accaccgtta ttaaccgaca ggaactgatt
    36421 accgccatat ccgccgtaaa cgcccataaa gaaggtccct gcatttacct gactaccttc
    36481 gccgcttaca ttcccctgcg tataagtcag acgactggtg cccttaacat gaattttgcc
    36541 gccattggag acattaaaat agccttgatc atgtctcatg cctgagtttg actcatacaa
    36601 atcatcagca tataactcgc tgccagcatt ggagacattc aggaaccccg tacctttcag
    36661 caaaaagtta agcgggccat tgattgttac ctttcctccg gtaatatcca acccaccacc
    36721 agaagcgaga gtcagacgcg gacgggaagt atctgtcgta ttgattacca gattggcatt
    36781 gtcagtcacc gtaatacggt aatcgatgct ctggttctct ttgccaaaat caatagcttt
    36841 atcctggttt acgatgacat catcggcgaa taccagagat gaatataaac ttatcgcgct
    36901 aattaaaaca gcccgggact tttttccttt tactctgctc cccagctccg agcagacgct
    36961 ccaacagtta cgggacctgt tccactttag ccgatatatt ttattcatca caatctccag
    37021 caatacatcc aaataaaacc aagattgcat aaacagcaac gcattaaatg ccattctggt
    37081 tactgttcat cgcatataaa aattcaaaaa ttttgcatag cattggcaac cacatatctg
    37141 agtatgtagt tcagagggta ataaaatagc aagttattac ttaaaactta ttttaagtta
    37201 acactcttaa ctttatcatt acattcctcc cagtctctaa tacctgcgca ttatatgtat
    37261 ccaggaactc aaatcaactc aaaatataaa caaaagcaag aggaatacat ttaatataca
    37321 gccgataaaa gcggtaactc ttattaggta tcaaaatata agaatcgtct tattaataaa
    37381 tatataacac tataggactg aatccattca gctaatacaa aataaaatcg ttaattaata
    37441 aaatataaaa tcatgaagtg atacaagagg aattgcagga tatgacatga caatcatata
    37501 taatatatgc ataatttttt acagcgagtc aatgattccc gcgccatatg cagacatggc
    37561 gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca cgctatggcc cggcgcttca
    37621 cgatggagat gaataaagtt aaggtgccgc tcgtactggt caagaatatc ggtgatcacc
    37681 tgctctttgc tgtagtccat caggtcgttg ccctggctgc cttctaacag gaaggtttcc
    37741 agccggtagt aagtcgattt accgctacgt gcgcggtagg taaagcccgg caccgaatat
    37801 tgctgcggcc aaatctgata gacaaagttt tgttcttcgc ccatatgcac caacaaatcc
    37861 agatgcccca actgttgtcc ctcttccggc ggcaggcttt ttagctccac gtatgcgccg
    37921 cgcaaccgca gctcctgcgc cacttcttcc attgccgggt aacagaccgt ctccatcatc
    37981 tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg agagacgttt tttccagctc
    38041 aggcgatcct gaagccccag cggtcgcggt gcggtatcgc ggttggcact ttcacggcgg
    38101 taatcttcta ccttcagaga tttatacaac cccgccatca cgaagaagat cacaaagctg
    38161 aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg atatcccgtt ggtcatcagc
    38221 atgccgagcg tcagcaggcc aatcgccacc gaccagaaga cgcgcagcca gccgggggcg
    38281 tcgctgttga tatctttaag ctgcgaggtg aaattcccca gcaccagcgc accggagtcc
    38341 gccgaggtca cataaaacag caggccagta atggtggcga cggaggcgct aaaggtaaac
    38401 gccggatact gcgccagcag gctgtagaag ccgcgctccg gatggaccat cgcttcctcg
    38461 gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc tattgccgaa caccgagagc
    38521 cataacagcg tgaaggtaaa cggaataatc aacgtgccca gcacgaactg gcgaatggta
    38581 cgcccacgcg agatacgcgc caggaacaag ccgacaaacg gcgaccatgc cacccaccat
    38641 gcccagaaga agagcgtcca gttattcatc cactcaactg gacggtcgaa ggcaaaactg
    38701 ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa cattcagcac cagcgcatta
    38761 agcaggaacg aggtgtcgcc cataaacaat acgaacagga tcaatcccag cgccagcgca
    38821 acgttaagtt ccgataacac acgaatcccc ttatcgacac cggaggtgac agagatcgtg
    38881 gcgattatca ccgacaaggc gatcagtgcc gcttttgccg ccatcgaatc gggaatatca
    38941 aacagtacgc tcaagccata gttaagctgc accacaccga taccgagcgt agtggcaata
    39001 ccgaagatgg tgccgatcac cgctgcaata tccactgagt gccctatcgg cccgttaatc
    39061 cgtttaccga agatcgggta cagcgccgag cggatggtga gcggcaaatt ataacgatag
    39121 ctaaagtatc cgagcgccat gcccatcagc gcatacatcg accagccggt taagccgtag
    39181 tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa tcgtctgtcc cgcgccttcc
    39241 ggcggctgca tatactgcgt taccggttcg gctacggaga agaacatcag gtcgataccg
    39301 atcccggcag caaacagcat cgccgcccaa ctcagcaggc tgaattccgg tttggattgt
    39361 tctggcccga gcttcaccga accaaaacgc gaacaagcga tacagaccac aaagacaata
    39421 tagagcgttg ccgccagtag atagtaccaa ccgaaggttt tagaaaccca gtccagcgtg
    39481 cggccaatcc acagggccga gaagtcgcga aacaggatcg ttgtcaggga aaacaacaaa
    39541 atcagtccgg cggaggtgta aaacaccacc ggattgattt tgtccttttc cctgctgtgt
    39601 gaaaggtctg tcatccagta tccccactgt tattgttacc atttaaaatc aaattcgtaa
    39661 caattaagac acattttata ttgaacgtcc aatcaataac cgctttaata gataaacaac
    39721 gctgatgaat ggagtggcga aaatgcccaa attggggatg cagtcgatcc ggcgcagaca
    39781 actgatcgac gccacactgg aagcaataaa tgaagtgggc atgcacgatg caacgatcgc
    39841 gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc agccactatt tcagggacaa
    39901 aaatggtctg ctggaagcaa ccatgcgcga catcaccagt cagttacgtg acgcggtttt
    39961 gaatcgatta catgcacttc cgcagggcag tgcagagcag cgattacagg cgattgttgc
    40021 cggaaacttc gatgaaactc aggtgagcag tgcggcgatg aaagcctggc tggcgttctg
    40081 ggccagcagt atgcatcagc cgatgctcta tcgtttacag caggtcagca gccgccgctt
    40141 gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg cgcgaacagg cacaggaagc
    40201 aggctacggc cttgccgcgc tgattgacgg attatggctg cgcgcggcac tgagcggcaa
    40261 accgctggat aaacctctcg cacactcgct gacccgtcac tttattactc agcatttacc
    40321 caccgattaa ccgaggagac gtgatgtccc gaatggcaga acagcagctt tatatacatg
    40381 gtggttatac ctccgccacc agcggtcgca ccttcgagac cattaacccg gccaacggta
    40441 acgtgctggc gaccgtgcag gccgccgggc gcgaggatgt cgatcgcgcc gtgaagagtg
    40501 cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat ggaacgctcg cgtattctgc
    40561 gtcgggccgt tgatattctg cgtgaacgca atgacgaact cgcaaaactt gaaaccctcg
    40621 acaccggaaa agcatattcg gaaacctcaa ccgtcgatat cgttaccggt gcggacgtgc
    40681 tggagtacta cgccgggctg atcccggctc tggaaggcag ccagatcccg ttgcgtgaga
    40741 cgttatttgt ttatacccgc cgcgaacctc tgggtgtggt ggcggggatt ggcgcatgga
    40801 actacccgat tcagattgcc ctgtggaaat ccgccccggc gctggcggca ggcaacgcaa
    40861 tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt aaagctggct gaaatttaca
    40921 gcgaagcggg cctgccggac ggcgtattta acgtgttgcc gggggtgggc gcggagaccg
    40981 ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc atttaccggc ggtgtcgcca
    41041 gcggcaaaaa agtgatggct aactcggcgg cctcttccct gaaagaagtg accatggaac
    41101 tgggcggtaa atcaccgctg atcgttttcg atgatgcgga tctcgatctc gccgccgata
    41161 tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg taccaatggc acccgcgtct
    41221 tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat tctggcgcgc gttgagcgca
    41281 ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg cccgctggtc agcttcccgc
    41341 atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga ggaaggcgcg cgcgtactgt
    41401 gcggcggcga tgtactgaaa ggcgatggct tcgataacgg cgcatgggtt gcaccgaccg
    41461 tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga agagatcttc ggtccggtga
    41521 tgtcgcttct cacttatgaa tctgaggacg aagtgattcg ccgcgccaat gataccgact
    41581 acggcctggc ggcgggcatc gtgacggcgg acctgaaccg cgcgcatcgc gtcattcatc
    41641 agctggaagc gggtatttgc tggatcaaca cctggggcga atccccggca gagatgcccg
    41701 ttggcggcta caaacactcc ggcattggtc gcgagaacgg cgtgatgacg ctccagagtt
    41761 acacccaggt gaagtccatc caggttgaga tggctaaatt ccagtccata ttctaaccag
    41821 gaggtttatt tgcaatttga ctacatcatt attggtgccg gctcagccgg caacgttctc
    41881 gctacccgtc tgactgaaga tccgaatacc tccgtgctgc tgcttgaagc gggcggcccg
    41941 gactatcgct ttgacttccg cacccagatg cccgcagcgc tggcgttccc actacagggc
    42001 aaacgctaca actgggccta tgaaacagag cctgaaccgt ttatgaataa ccgccgcatg
    42061 gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga tcaacggcat gtgctacatc
    42121 cgtggcaatg cgctggatct cgataactgg gcgcaagaac ccggtctgga gaactggagt
    42181 tatctcgact gcctgcccta ctaccgcaag gccgagactc gcgatgtggg cgagaacgac
    42241 taccacggtg gcgatggccc ggtgagcgtc accacctcca aacccggcgt caatccgctg
    42301 tttgaagcga tgattgaagc gggcgtgcag gcaggctacc cgcgcacgga cgatctcaac
    42361 ggctatcagc aggaaggttt tggcccgatg gatcgcaccg tcacgccgca gggccgtcgc
    42421 gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc gccctaacct gaccattcgt
    42481 actcacgcta tgaccgatca catcattttt gacggcaaac gcgcggtggg cgtcgaatgg
    42541 ctggaaggcg acagcaccat cccaacccgc gcaacggcca acaaagaagt gctgttatgt
    42601 gcaggcgcga ttgcctcacc gcagatcctg caacgctccg gcgtcggcaa cgctgaactg
    42661 ctggcggagt ttgatattcc gctggtgcat gaattacccg gcgtcggcga aaatcttcag
    42721 gatcatctgg agatgtatct gcaatatgag tgcaaagaac cggtttccct ctaccctgcc
    42781 ctgcagtggt ggaaccaacc gaaaatcggt gcggagtggc tgtttggcgg cactggcgtt
    42841 ggtgccagca accactttga agcaggtgga tttattcgca gccgtgagga atttgcgtgg
    42901 ccgaatattc agtaccattt cctgccagta gcgattaact ataacggctc gaatgcagtg
    42961 aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct cgccaagccg tgggcatgtg
    43021 cggattaaat cccgcgaccc gcaccagcat ccggcgattc tgtttaacta catgtcgcac
    43081 gagcaggact ggcaggagtt ccgcgacgca attcgcatca cccgcgagat catgcatcaa
    43141 cccgcgctgg atcagtatcg tggccgcgaa atcagccccg gcacggaatg tcagacggat
    43201 gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg ccttccatcc gtgcggtacc
    43261 tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg aaggccgcgt acacgggtta
    43321 gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga ttatcaccgg gaatttgaac
    43381 gccacgacaa ttatgattgg cgagaaaata gcggatatga ttcgtggaca ggaagcgctg
    43441 ccgaggagca cggcgggata ttttgtggca aatgggatgc cggtgagagc gaaaaaaatg
    43501 agtcgtgatg tgaactgact caagaaccga tcagattgag agttaccgtt ccaggcatgg
    43561 ggacagaatc gttatataaa acataggggt aactctcatg cttcatatcc agtattcatg
    43621 atgctggctt tttgctgata tttaacccga acattcctga tacagactat taatacaacc
    43681 ttatctgcga cagattttat tttctggata tattatgcgt gaacaaatca aacaggatat
    43741 cgatctgatt gagattttat tttatctgaa gaaaaaggtt cgtgttatcc tttttattat
    43801 agctatctgt atggcaatga tgcttttatt tctgtatatc aataaagata atataaaagt
    43861 gagttacagc ctaaaaataa accagacaac accagacata cttgttaact gtgatagcaa
    43921 taataacttt gcctgtcaga ccgcaatgac taaagatgtt atacagcgaa ttactacatt
    43981 ttttcacact agcccagatg tcaagaacag agaaataaag ctggaatggt catgagataa
    44041 gagagattta ccaactgctg aatcggaaat atctcgcgtg caggcgtcta ttatcaaatg
    44101 gtatgcgtca gaataccata atggcaggca agttctcgat gagatacaaa taccttcagc
    44161 aattaacagt gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc
    44221 gaacggtgat ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc
    44281 catttgcctg gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt
    44341 caaaaaaatg gtagatgaat actaacaaaa ctctgggcag taataaacaa cattctgata
    44401 gagagtgcct gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat
    44461 ggatgcctgt ctttttcact gtaaatatcc tggcattact aatgccagga tatttacaga
    44521 agagcagcat aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa
    44581 cctcgtatgc cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat
    44641 aatcagatac accgctttca ccgggccatg aaccccgacg actttgataa gctcaatatc
    44701 cgccgttgaa ctagggccgc taatgatgtt aatgcaggac ggcattcgtt caccggcctg
    44761 cgctttctga tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg
    44821 caggataaaa agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga
    44881 aaaaagaacc acgcctcccg attcggttaa accatattca gcatacacga cacccacttt
    44941 agcctgctct gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt
    45001 gcattcctgc tgcaaacgtt cgctaatccc caattcctct agcctcgtgt caccgctaat
    45061 cacgaccgac tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc
    45121 ctcgctggtc agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc
    45181 acagcgctgc tgttggttaa gtgcggtaag ccgctcgtta gcatagttgt taagcggcgc
    45241 atcttctgct tgcggttcaa gtcgcaacgg gcgacccagt gcctgagcaa cgttattcaa
    45301 aaattcgctc cgattatcca ttctttttct cctgcgccag atgtttctta aaccaactac
    45361 ggaaactctc tccgttagct tcaggaagat cgcgtgcttc catccagtca ctaatcgcgc
    45421 caaatttgag tggtgttttg ccgccattga taaaccagct tgccgcatga gcaccggcca
    45481 tcatcccgac tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc
    45541 gttgctctgc ttttgcggtg atcccttgtt cagccatcac ccgacgatga cgcaaaatca
    45601 gttttgacag cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg
    45661 cgtagggtaa atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa
    45721 ttggccctgg ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag
    45781 tattcataca agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca
    45841 cctcagaacg cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc
    45901 ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac
    45961 tgcgcgccag catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca
    46021 ttcccatcac tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat
    46081 tggtcaccag gcataccgaa cctgtctctg ccaccgcgaa attacagccg gtaataccta
    46141 tttcagcact gaggaaatct tcgcggattt tttgccggat gaataaggtc atcgcttcag
    46201 gcgtttccgg cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat
    46261 ctttatgaat tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat
    46321 attcacccag atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac
    46381 caatctcttc ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg
    46441 caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt tgcaaaccag acgtgaccgc
    46501 cgttttgcgt cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat
    46561 gatcacgtat ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca
    46621 ttttttgccg atttgcccca atacgctgct gcgcgtttgc caccgctttg cgcatgatcg
    46681 gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca
    46741 tctttatatc ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc
    46801 ccttcccgtt gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa
    46861 tactcagggc ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt
    46921 tcggccattt tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag
    46981 gtcaacagct ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc
    47041 agcttacggg ccaggctaca agatgggtga tatactgctc tcccttgcaa actggcacct
    47101 acatcgacta cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca
    47161 accttttcgg cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt
    47221 acggcatagg tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg
    47281 gcgatcagat ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc
    47341 tgaccgcagc atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc
    47401 agcacggagt ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa
    47461 ttgacattca caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc
    47521 caccggggga tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc
    47581 acccctcatt tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat
    47641 tttatttctt tcggcatcgc tcacatttta tggggaaatc gacgttcatt tttggcataa
    47701 gaaatccatt taaaaaggct acatccctta tacctattca gagctactta ttcaggggtt
    47761 attgagatat acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa
    47821 tagtcggggt tttatttaat tgaagcaatt tagtttgcat ttttatttaa aaactgagct
    47881 agagtccatc agaaaaaaat atataaagcc caaaccaccg ttggaaatag aaatagttga
    47941 aaggctcaca actcccggaa tgcggattat ctttgccctg atttctggcg gttttattca
    48001 gggtgcaagc tgtctgaccc tttcccgata ttctcccgga gtacaaccaa actcgcggac
    48061 aaacgccttg tgaaaagatg attcactggc atagcctact gactcagcga tcaccacaac
    48121 agggagcatt tcccgggaaa acatctgggc cgctatttgt agacgcaact ttgttaatac
    48181 agccagcggc gtggttccgg aaacatcacg gaaaagctgg gcaaaacttg cccgggacat
    48241 gtgagcgatg ctggccagcg attcgacggt ccaggcgtgt cctggcattt ccagcatttg
    48301 ctgtattacc gcaccaagac gtggatgcag aagcaaactg agaatgtttt tctcagtatt
    48361 aacctgtgca atccactcac gcaccgcgag ggtaaagaat gtagcgcaga tctggctaca
    48421 cagtgcatcc actcccggca tcgccgatct ggattcctgt tgtaaaaacg ggatcgcctc
    48481 cctcagccag ttatattcca cgctgtggtt aaccggtgct aaaaacagcg tttccggcaa
    48541 agacgttaaa aaataacgcg ccgaatgttg caaccgaaga gtgccgcaga caatacaggt
    48601 cgattcatta tcgacatgac tcaggcgatg agctgaattt tgtggtagca ggaccacatt
    48661 tcccgggcgt aatgtaaaaa tctctcccgt cggcatttcc agcttcgccg ctccttgcgt
    48721 taacgcatgc caacgaataa ccgataattc cccggcacca tgtggaagct gccagtcact
    48781 tcctaacacg caattcttat cgatcgttcc ttgtggagcg ttaagcatca acaaacggct
    48841 aagggcatcc atatcagact cctgagtggt ttttgattac aaacaccaac aatcttagta
    48901 aataactaat ctaataccat caaatggtta atcatgattt ttgttttgtg attctgatta
    48961 agaaaaagaa aataccagac gataagacaa aatatcaagc gttgtgaaga aatggtattt
    49021 gctcttttgc gtctatagtc atgatgtcaa atgaacgcgt ttcgacagga aatcatcatg
    49081 aataaatatc aggcagttat tattggtttt ggcaaggctg gaaaaacatt agccgtcacg
    49141 ctggcaaaag caggttggcg agtggctctc atcgaacaat caaatgcaat gtatggcggg
    49201 acctgtatta atatcggctg catcccaacc aaaacattgg ttcatgacgc acagcagcac
    49261 acagattttg tccgtgccat acagcgtaaa aatgaagtgg ttaatttttt acgtaataag
    49321 aattttcata atcttgcgga tatgcccaat atcgacgtga tcgacggcca ggcggagttt
    49381 atcaataatc atagcctgcg tgttcatcgg cctgagggaa atctggaaat tcatggcgag
    49441 aaaattttta ttaataccgg tgcacaaacc gtggttccgc caattcctgg aattaccacc
    49501 acgcctggtg tatatgacag caccggatta cttaatctaa aagaattgcc tgggcattta
    49561 ggtattttgg gcggcggata tattggcgtt gagttcgcct ctatgttcgc taattttggc
    49621 agcaaggtaa ccattttaga agcagcttcg ctgtttttgc ctcgggaaga tcgggatatt
    49681 gctgataata tcgcgacgat tttacgcgat cagggcgtcg atattatcct caatgcccat
    49741 gtggagcgaa tcagtcacaa tgaaaatcaa gtgcaagtgc atagcgagca cgcccaactg
    49801 gcggtggatg cactgttaat agcttccggt cgtcaaccgg ctaccgcttc gttacatcca
    49861 gaaaatgccg gtatcgcagt aaacgagcgc ggggcaattg tcgttgacaa gcaattacat
    49921 accaccgcag acaatatttg ggcgatggga gatgttaccg gcgggctgca atttacttat
    49981 atttcactgg atgattaccg cattgtgcgt gatgagttgc tgggagaagg cagacgtagt
    50041 actgatgatc ggaaaaatgt gccttattcc gtattcatga caccgccact gtccagggtt
    50101 ggtatgacag aagaacaggc cagagagagt ggtgctgata ttcaggtggt gacattgcct
    50161 gtagctgcaa ttccgcgtgc cagagtgatg aatgatactc gtggggtatt aaaagcgatt
    50221 gttgataata aaacccaacg tatattaggg gcatcactgc tgtgtgttga ctcccacgag
    50281 atgattaata tagtgaaaat ggtgatggat gccgggctgc cttatagcat attacacgat
    50341 cagatattta ctcatccgtc gatgagcgaa tcactcaatg atctattttc attagtcaaa
    50401 taaactcaaa atcagacgcc agaacaaata ttctggcgtc tcagagaaaa gaatcttatt
    50461 aattccctgt tactctataa cttcttaatc acttcattga tggtatttta tatgttcaaa
    50521 aaatctgttt tatttgcaac actattatct ggcgttatgg cattttccac caatgcagat
    50581 gataaaataa ttctgaaaca tatcagcgtc tcgtcagtat cagcatcacc gacagttctg
    50641 gaggatacca ttgctgatat agccagaaaa tataatgctt catcctggaa agtcacatcg
    50701 atgcgaattg ataataattc aaccgcaaca gcagtattgt ataaataagg atgttcacaa
    50761 tggaaaaata cctgcacctg ttaagtcggg gagataaaat tggcctgaca ttgattcgtc
    50821 tgagtattgc aattgttttt atgtggattg ggttattaaa gtttgtccct tacgaggcag
    50881 acagcattac accattcgtc gcaaacagtc cactaatgtc gttcttttat gaacacccgg
    50941 aagactataa acagtatctg actcacgaag gcgaatacaa accagaagca agggcatggc
    51001 aaacggccaa taatacctat ggtttttcca acggtcttgg cgtcgtggag gtgattattg
    51061 ctctgctggt tttggctaat cctgtcaatc gctggttagg tttattggga ggactgatgg
    51121 catttaccac gccgctggta acactctcat ttttaatcac caccccggag gcatgggtac
    51181 ccgcattggg tgacgctcat catggtttcc cttatttatc cggtgctggt cgcctggtat
    51241 tgaaagatac tctgatgctg gcaggtgctg tcatgataat ggcagattcg gcgcgggaaa
    51301 ttcttaaaca acgcagtaat gaatccagtt caacgttaaa aactgaatat tgataatccg
    51361 aacactgtct gttgttcact ttttcaggcg tcaggccccc tttctgatga tgaagcctga
    51421 cgccatatta ccggagatat gacagagtga agatcaatat cactcccctg ttaaaagatt
    51481 cttgtcgtct caggtaaaat atatgattaa tatcaatcat gtatttttac agcaagacaa
    51541 ccacgaacaa attcaggatt aaaatgtttt accgactccc caacataacc caaatcaagc
    51601 gaactaattt gtgccatttc tttctctgtc agtgagaaat cccagatagc aaaattttct
    51661 tcgatacgct gttgatgtgt cgatttcgga ataacgacca ctccccgttg aatattccaa
    51721 cgcagaatga cttgagaaat cgatttttga tgggcatctg caatactctg aagcagatta
    51781 ttttcaaagg gtttatgtcg tccaccacct aacggagccc atgcttcagg ctgcacgtta
    51841 taatacttca tggttgccag cgcctctggt tgggcaaaat agggatgcaa ctcgacctgg
    51901 ttgaccatcg gtttaactct taccgtttca caaaagttcg ccagaacatg aggatagaaa
    51961 ttggaaaccc caattgcttt taatttgccc tcttcatagg catcttcgag tgcacgccac
    52021 gcactgaaat aatcgcgcat agcctggtgc aataaataga ggtcgaagta ctctagtccc
    52081 gattttttta atgatgcttc aatacctgct gctgccgtat cctgattcaa catatcctgc
    52141 acccacaatt tagacgttat aaataactca tctcgggtac ataaaccttc agatatagct
    52201 tcacgaacgg cttcaccgac ggcatcttca ttaccgtata ccgctgcagt gtcgatgagt
    52261 cgataacctg tgcggatagc gttcaacact gactgtttgc acacgttttt atcggtaacc
    52321 tgaaaaacac caaatcccat catcggcatt ttcagattgt tacttaatac ggaaaattcc
    52381 actgtcagtc cctccattag gcatgaacaa tgagtctacg ttaaaacgta acctcaaatt
    52441 agtacgtgga ttttgatatc acttatgcaa aaaattcatt aataatgcag gactgaaacc
    52501 tctctatttt cggggacaac gaagcagacg ctaccagtgc ttttgccttc gcccttgctg
    52561 tttttgatac acttagggcc cagggcataa cgaaaatgtg cgatatgacg aagaatcaat
    52621 ggagaatgtg atgatcaggc agaagattct acagcagctc ctggagtgga ttgagtgcaa
    52681 tcttgagcac cctatttcaa tcgaagatat cgcacagaaa tctggctaca gcagacgcaa
    52741 catccagctt ctgttccgaa atttcatgca tgtgcctttg ggagaataca ttcgcaaacg
    52801 aaggctttgt cgtgccgcca ttcttgtccg gctcaccgcg aaatctatgc ttgatattgc
    52861 actctctttg cattttgatt cacagcagtc attcagccgc gaatttaaaa agttattcgg
    52921 ctgctctccc cgtgtatacc gccaccgtga ttattgggat ctcgcaaata tcttcccttc
    52981 ttttttaata cgtcaacagc aaaagaccga gtgtagatta gtcaactttc ctgagacacc
    53041 tatttttggc aattcattta aatatgatat tgaagtgtcg aataaatcac cggatgaaga
    53101 agtcaaactg cgacgtcatc atttagccag atgtatgaat aattttaaga
//
LOCUS       DAHBHC010000027        51403 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHBHC010000027 DAHBHC010000000
VERSION     DAHBHC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.027"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..3053
                     /gene="sbcC"
                     /locus_tag="M2A35_001989"
     CDS             <1..3053
                     /gene="sbcC"
                     /locus_tag="M2A35_001989"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCB7198296.1"
                     /translation="LFAITGPTGAGKTTLLDAICLALYHETPRLSNVSQSQNDLMTRD
                     TAECLAEVEFEVKGEAYRAFWSQNRARNQPDGNLQVPRVELARCADGKILADKVKDKL
                     ELTATLTGLDYGRFTRSMLLSQGQFAAFLNAKPKERAELLEELTGTEIYGQISAMVFE
                     QHKSARTELEKLQAQASGVALLTPEQVQSLTASLQVLTDEEKQLLTAQQQEQQSLNWL
                     TRLDELQQEASRRQQALQQALAEEEKAQPQLAALSLAQPARNLRPHWERIAEHSAALA
                     HTRQQIEEVNTHLQSTMALRASIRHHAAKQSAELQQQQQSLNTWLQEHDRFRQWNNEL
                     AGWRAQFSQQTSDREHLRQWQQQLTHAEQKLNALAAITLTLTADEVATALAQHAEQRP
                     LRQHLVALHGQIVPQQKRLAQLQVAIQNVTQEQTQRNAALNEMRQRYKEKTQQLADVK
                     TICEQEARIKTLEAQRAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLALENEV
                     KKLGEEGAALRGQLDALTKQLQRDENEAQSLRQDEQALTQQWQAVTASLNITLQPQDD
                     IQPWLDAQDEHERQLRLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALAGYAL
                     TLPQEDEEESWLATRQQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEET
                     VALDNWRQVHEQCLALHSQQQTLQQQDVLAAQSLQKAQAQFDTALQASVFDDQQAFLA
                     ALMDEQTLTQLEQLKQNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQ
                     ELAQTHQKLRENTTSQGEIRQQLKQDADNRQQQQTLMQQIAQMTQQVEDWGYLNSLIG
                     SKEGDKFRKFAQGLTLDNLVHLANQQLTRLHGRYLLQRKASEALEVEVVDTWQADAVR
                     DTRTLSGGESFLVSLALALALSDLVSHKTRIDSLFLDEGFGTLDSETLDTALDALDAL
                     NASGKTIGVISHVEAMKERIPVQIKVKKINGLGYSKLESTFAVK"
     gene            3179..4363
                     /gene="araJ"
                     /locus_tag="M2A35_001990"
     CDS             3179..4363
                     /gene="araJ"
                     /locus_tag="M2A35_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCB7198297.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4506..5414)
                     /gene="mak"
                     /locus_tag="M2A35_001991"
     CDS             complement(4506..5414)
                     /gene="mak"
                     /locus_tag="M2A35_001991"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCB7198298.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            5539..6450
                     /gene="rdgC"
                     /locus_tag="M2A35_001992"
     CDS             5539..6450
                     /gene="rdgC"
                     /locus_tag="M2A35_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCB7198299.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(6528..6611)
                     /locus_tag="M2A35_001993"
     CDS             complement(6528..6611)
                     /locus_tag="M2A35_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198300.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(6608..6949)
                     /locus_tag="M2A35_001994"
                     /pseudo
     CDS             complement(6608..6949)
                     /locus_tag="M2A35_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(7097..7381)
                     /gene="ppnP"
                     /locus_tag="M2A35_001995"
     CDS             complement(7097..7381)
                     /gene="ppnP"
                     /locus_tag="M2A35_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCB7198301.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(7453..8130)
                     /locus_tag="M2A35_001996"
     CDS             complement(7453..8130)
                     /locus_tag="M2A35_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCB7198302.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDIQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(8388..8579)
                     /gene="yaiA"
                     /locus_tag="M2A35_001997"
     CDS             complement(8388..8579)
                     /gene="yaiA"
                     /locus_tag="M2A35_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCB7198303.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(8629..9153)
                     /gene="aroL"
                     /locus_tag="M2A35_001998"
     CDS             complement(8629..9153)
                     /gene="aroL"
                     /locus_tag="M2A35_001998"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCB7198304.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(9336..9794)
                     /locus_tag="M2A35_001999"
     CDS             complement(9336..9794)
                     /locus_tag="M2A35_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCB7198305.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            9914..10723
                     /gene="proC"
                     /locus_tag="M2A35_002000"
     CDS             9914..10723
                     /gene="proC"
                     /locus_tag="M2A35_002000"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCB7198306.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(10740..11855)
                     /gene="adrA"
                     /locus_tag="M2A35_002001"
                     /gene_synonym="dgcC"
     CDS             complement(10740..11855)
                     /gene="adrA"
                     /locus_tag="M2A35_002001"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCB7198307.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSYLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(11957..12277)
                     /gene="psiF"
                     /locus_tag="M2A35_002002"
     CDS             complement(11957..12277)
                     /gene="psiF"
                     /locus_tag="M2A35_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCB7198308.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(12396..13811)
                     /gene="phoA"
                     /locus_tag="M2A35_002003"
     CDS             complement(12396..13811)
                     /gene="phoA"
                     /locus_tag="M2A35_002003"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCB7198309.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(13912..14172)
                     /gene="iraP"
                     /locus_tag="M2A35_002004"
     CDS             complement(13912..14172)
                     /gene="iraP"
                     /locus_tag="M2A35_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCB7198310.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(14354..14557)
                     /locus_tag="M2A35_002005"
     CDS             complement(14354..14557)
                     /locus_tag="M2A35_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198311.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            14635..15729
                     /gene="ddlA"
                     /locus_tag="M2A35_002006"
     CDS             14635..15729
                     /gene="ddlA"
                     /locus_tag="M2A35_002006"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCB7198312.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(15753..15965)
                     /locus_tag="M2A35_002007"
     CDS             complement(15753..15965)
                     /locus_tag="M2A35_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCB7198313.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            16225..16533
                     /locus_tag="M2A35_002008"
     CDS             16225..16533
                     /locus_tag="M2A35_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCB7198314.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(16592..17686)
                     /gene="yaiW"
                     /locus_tag="M2A35_002009"
     CDS             complement(16592..17686)
                     /gene="yaiW"
                     /locus_tag="M2A35_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCB7198315.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(17699..18919)
                     /gene="sbmA"
                     /locus_tag="M2A35_002010"
     CDS             complement(17699..18919)
                     /gene="sbmA"
                     /locus_tag="M2A35_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCB7198316.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGEWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            19271..20428
                     /gene="ampH"
                     /locus_tag="M2A35_002011"
     CDS             19271..20428
                     /gene="ampH"
                     /locus_tag="M2A35_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCB7198317.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(20429..21052)
                     /gene="iprA"
                     /locus_tag="M2A35_002012"
     CDS             complement(20429..21052)
                     /gene="iprA"
                     /locus_tag="M2A35_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCB7198318.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(21140..24118)
                     /locus_tag="M2A35_002013"
     CDS             complement(21140..24118)
                     /locus_tag="M2A35_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7198319.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMGESSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANST
                     IDDDYEGLYFTDSYLDGDVTKYTNETFHTPAGEGEEYAGLFANGGVGLGLAVNLDVES
                     NINISNNSRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFYG
                     NSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFYP
                     QGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSKWVGSATTSANVDDESTASTDWY
                     DVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNNG
                     SQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNGY
                     YTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIHS
                     SDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVV
                     AATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
     gene            24642..25616
                     /gene="hemB"
                     /locus_tag="M2A35_002014"
     CDS             24642..25616
                     /gene="hemB"
                     /locus_tag="M2A35_002014"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCB7198320.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(25722..26573)
                     /gene="tauD"
                     /locus_tag="M2A35_002015"
     CDS             complement(25722..26573)
                     /gene="tauD"
                     /locus_tag="M2A35_002015"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCB7198321.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(26570..27397)
                     /gene="tauC"
                     /locus_tag="M2A35_002016"
     CDS             complement(26570..27397)
                     /gene="tauC"
                     /locus_tag="M2A35_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCB7198322.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGPSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(27394..28161)
                     /gene="tauB"
                     /locus_tag="M2A35_002017"
     CDS             complement(27394..28161)
                     /gene="tauB"
                     /locus_tag="M2A35_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCB7198323.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(28174..29136)
                     /gene="tauA"
                     /locus_tag="M2A35_002018"
     CDS             complement(28174..29136)
                     /gene="tauA"
                     /locus_tag="M2A35_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7198324.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IITAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDYAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            29752..30423
                     /locus_tag="M2A35_002019"
     CDS             29752..30423
                     /locus_tag="M2A35_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCB7198325.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            30433..31629
                     /locus_tag="M2A35_002020"
     CDS             30433..31629
                     /locus_tag="M2A35_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCB7198326.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            31607..32188
                     /locus_tag="M2A35_002021"
     CDS             31607..32188
                     /locus_tag="M2A35_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCB7198327.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            32190..32963
                     /locus_tag="M2A35_002022"
     CDS             32190..32963
                     /locus_tag="M2A35_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCB7198328.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            33148..33423
                     /gene="frmR"
                     /locus_tag="M2A35_002023"
     CDS             33148..33423
                     /gene="frmR"
                     /locus_tag="M2A35_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCB7198329.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            33458..34567
                     /gene="frmA"
                     /locus_tag="M2A35_002024"
     CDS             33458..34567
                     /gene="frmA"
                     /locus_tag="M2A35_002024"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCB7198330.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            34660..35493
                     /gene="fghA"
                     /locus_tag="M2A35_002025"
     CDS             34660..35493
                     /gene="fghA"
                     /locus_tag="M2A35_002025"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCB7198331.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(35618..36157)
                     /locus_tag="M2A35_002026"
     CDS             complement(35618..36157)
                     /locus_tag="M2A35_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCB7198332.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(36259..37470)
                     /gene="mhpT"
                     /locus_tag="M2A35_002027"
     CDS             complement(36259..37470)
                     /gene="mhpT"
                     /locus_tag="M2A35_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCB7198333.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(37745..38758)
                     /gene="dmpG"
                     /locus_tag="M2A35_002028"
     CDS             complement(37745..38758)
                     /gene="dmpG"
                     /locus_tag="M2A35_002028"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCB7198334.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(38755..39705)
                     /gene="mhpF"
                     /locus_tag="M2A35_002029"
     CDS             complement(38755..39705)
                     /gene="mhpF"
                     /locus_tag="M2A35_002029"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCB7198335.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(39702..40511)
                     /gene="mhpD"
                     /locus_tag="M2A35_002030"
     CDS             complement(39702..40511)
                     /gene="mhpD"
                     /locus_tag="M2A35_002030"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCB7198336.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(40521..41387)
                     /gene="mhpC"
                     /locus_tag="M2A35_002031"
     CDS             complement(40521..41387)
                     /gene="mhpC"
                     /locus_tag="M2A35_002031"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCB7198337.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(41405..42349)
                     /gene="mhpB"
                     /locus_tag="M2A35_002032"
     CDS             complement(41405..42349)
                     /gene="mhpB"
                     /locus_tag="M2A35_002032"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCB7198338.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(42351..44015)
                     /locus_tag="M2A35_002033"
     CDS             complement(42351..44015)
                     /locus_tag="M2A35_002033"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCB7198339.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            44092..45039
                     /gene="mhpR"
                     /locus_tag="M2A35_002034"
     CDS             44092..45039
                     /gene="mhpR"
                     /locus_tag="M2A35_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HCB7198340.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNFVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQDRKELIEMLASRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            45116..46198
                     /gene="lacI"
                     /locus_tag="M2A35_002035"
     CDS             45116..46198
                     /gene="lacI"
                     /locus_tag="M2A35_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCB7198341.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            46321..49395
                     /gene="lacZ"
                     /locus_tag="M2A35_002036"
     CDS             46321..49395
                     /gene="lacZ"
                     /locus_tag="M2A35_002036"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCB7198342.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRRV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVVSGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWNKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            49413..50702
                     /gene="lacY"
                     /locus_tag="M2A35_002037"
     CDS             49413..50702
                     /gene="lacY"
                     /locus_tag="M2A35_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012770342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HCB7198343.1"
                     /translation="MSARISRKEIHYVLFKKHKLLDVRFLFFFFYFFIMGAYFPFFPI
                     WLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPF
                     FIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGC
                     VGWALCASIVGIMFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHS
                     AFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYV
                     TTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLH
                     MFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQG
                     AYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA"
     gene            50768..51379
                     /gene="lacA"
                     /locus_tag="M2A35_002038"
     CDS             50768..51379
                     /gene="lacA"
                     /locus_tag="M2A35_002038"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HCB7198344.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
BASE COUNT        12591 a        13549 c        12832 g        12431 t
ORIGIN      
        1 ggctgtttgc cattaccggc ccaaccggtg cggggaaaac caccctgctg gacgccattt
       61 gtctggcgct gtatcacgaa actccgcgtc tctctaacgt ttcacaatcg caaaatgatc
      121 tcatgacccg cgataccgct gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag
      181 cgtaccgtgc attctggagc cagaatcggg cgcgtaacca acccgacggt aatttgcagg
      241 tgccacgcgt tgagctggcg cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag
      301 ataagctaga actgacagca acgttaaccg ggctggatta cgggcgcttc acccgttcga
      361 tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg
      421 aattgctcga ggagttaacc ggcactgaaa tctacgggca aatctcggcg atggtttttg
      481 agcagcacaa atcggcccgc actgagctgg agaagctgca agcgcaggcc agcggcgtcg
      541 cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta cttactgacg
      601 aagaaaaaca gttacttacc gcgcagcagc aagaacaaca atcactaaac tggttaacgc
      661 gtctggacga attgcagcaa gaagccagcc gccgtcagca ggccttgcaa caggcgttag
      721 ccgaagaaga aaaagcgcaa cctcaactgg cggcgctcag tctggcacaa ccggcacgaa
      781 atcttcgtcc gcactgggaa cgcatcgcag aacacagcgc ggcgctggcg catactcgcc
      841 agcagattga agaagtaaat actcacttac agagcacaat ggcgcttcgc gcgagcattc
      901 gccaccacgc ggcgaagcag tcagcagaat tacagcagca gcaacaaagc ctgaatacct
      961 ggttacagga acacgaccgc ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc
     1021 agttctccca acaaaccagc gatcgcgagc atctgcggca atggcagcaa cagttaaccc
     1081 atgctgagca aaaacttaat gcgcttgcgg cgatcacgtt gacgttaacc gccgatgaag
     1141 ttgctaccgc cctggcgcaa catgctgagc aacgcccact gcgtcagcac ctggtcgcgc
     1201 tacatggaca gattgttccc caacaaaaac gtctggcgca gttacaggtc gctatccaga
     1261 atgtcacgca agaacagacg caacgtaacg ccgcacttaa cgaaatgcgc cagcgttata
     1321 aagaaaagac gcagcaactt gccgatgtga aaaccatttg cgagcaggaa gcgcgcatca
     1381 aaacgctgga agctcaacgt gcacagttac aggcgggtca gccttgtcca ctttgtggtt
     1441 ccaccagcca cccggcggtc gaggcgtatc aggcgctgga gcctggtgtt aatcagtctc
     1501 gattactggc gctggaaaac gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg
     1561 ggcaactgga cgccttaaca aagcagcttc agcgcgatga aaacgaagcg caaagcctcc
     1621 gacaagatga gcaagcactt actcaacaat ggcaagccgt cacggccagc ctcaatatca
     1681 ccttgcagcc acaggacgat attcaaccgt ggctggatgc acaagatgag cacgaacgcc
     1741 agctgcggtt actcagccaa cgacatgaat tacaagggca gattgccgcg cataatcagc
     1801 aaattatcca gtatcaacag caaattgaac aacgccagca gcagctctta acggcattgg
     1861 cgggttatgc actgacattg ccacaggaag atgaagaaga gagctggttg gcgacacgtc
     1921 agcaagaagc gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg caaaaccgta
     1981 ttcagcagct gacgccgatt ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg
     2041 aagaaactgt ggcgctggat aactggcggc aggttcatga acaatgtctc gcattacaca
     2101 gccagcagca gacgttacag caacaggatg ttctggcggc gcaaagtctg caaaaagccc
     2161 aggcgcagtt tgacaccgcg ctacaggcca gcgtctttga cgatcagcag gcgttccttg
     2221 cggcgctaat ggatgaacaa acactaacgc agctggaaca gctcaagcag aatctggaaa
     2281 accagcgccg tcaggcgcaa acgctggtca ctcagacagc agaaacgctg gcacagcatc
     2341 aacaacaccg acctgacggg ttggctctca ctgtgacggt ggagcagatt cagcaagagt
     2401 tagcgcaaac tcaccaaaag ttgcgtgaaa acaccacgag tcaaggcgag attcgccagc
     2461 agctgaagca ggatgcagat aaccgtcagc aacaacaaac cttaatgcag caaattgctc
     2521 aaatgacgca gcaggttgag gactggggat atctgaattc gctaataggt tccaaagagg
     2581 gcgataaatt ccgcaagttt gcccaggggc tgacgctgga taatttagtc catctcgcta
     2641 atcagcaact tacccggctg cacgggcgct atctgttaca gcgcaaagcc agcgaggcgc
     2701 tggaagtcga ggttgttgat acctggcagg cagatgcggt acgcgatacc cgtacccttt
     2761 ccggcggcga aagtttcctc gttagtctgg cgctggcgct ggcgctttcg gatctggtca
     2821 gccataaaac acgtattgac tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg
     2881 aaacgctgga taccgccctt gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg
     2941 gtgtgattag ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa
     3001 agatcaacgg cctgggctac agcaaactgg aaagtacgtt tgcagtgaaa taactattca
     3061 gcaggataat gaatacagag gggcgaatta tctcttggcc ttgctggtcg ttatcctgca
     3121 agctatcact ttattggcta cggtgattgg tagccgttct ggtggttgtg atggtggtat
     3181 gaaaaaagtc attttatctt tggctctggg cacgtttggt ttggggatgg ccgaatttgg
     3241 cattatgggc gtgctcacgg agctggcgca taacgtagga atttcgattc ctgctgccgg
     3301 gcatatgatc tcgtattatg cactgggggt ggtggtcggt gcgccaatca tcgcactctt
     3361 ttccagccgc tactcactca aacatatctt gttgtttctg gtggcgttgt gcgtcattgg
     3421 caacgccatg ttcacgctct cttcgtctta cctgatgctt gccattggtc gcctggtttc
     3481 cggctttccg catggcgcat tttttggcgt cggcgcgatc gtgttatcaa aaattatcaa
     3541 acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc gggatgacag tcgccaattt
     3601 gctgggcatt ccgctgggaa cgtatttaag tcaggaattt agctggcgtt acaccttttt
     3661 attgatcgct gtttttaata ttgcggtgat ggcatcggtc tatttttggg tgccggatat
     3721 tcgcgacgag gcgaaaggaa agctgcgcga acaatttcac tttttacgca gcccggcccc
     3781 gtggttaatt ttcgccgcca cgatgtttgg caacgcaggt gtgtttgcct ggttcagcta
     3841 cgtaaagcca tacatgatgt ttatttccgg tttttcggaa acggcgatga cctttattat
     3901 gatgttagtt gggctaggga tggtgctggg gaatgtgcta agtggcagga tttcaggacg
     3961 ttattcacca ctgcgcattg cagcagtaac tgactttatc attgtactgg cactgctgat
     4021 gctttttttc tgcggcggca tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc
     4081 gggattattt gccctttcag cacctctgca aatattgtta ctacaaaacg ccaaaggcgg
     4141 agagttatta ggtgccgcag gtgggcaaat agcgtttaac ctcggtagcg ccgtcggcgc
     4201 atattgcgga ggtatgatgc tgacgctggg gctggcatat aattacgtgg cgctgcctgc
     4261 cgccctgctt tcgtttgctg cgatgtcatc gttactgctg tatggtcgct ataagcgcca
     4321 gcaagcggcg gatagtccgg tgctggcgaa accactgggg tagattactg tcacggtgcg
     4381 cgttgcttat tgccggatgc ggcgtgaacg ccttatccgc cctacgcggt tccggcacat
     4441 tttgtaggcc tgataagacg cggcaagcgt cgcatcaagc atgatgcgcc aattgcctac
     4501 gttttttact cttgtggcca taaccacgca gcgccgcgta cgccgctgga atcaccgtgc
     4561 ttcgccttac gcaccggcgt ttcacattcg ccgccgaaga caaattgttt aatcaactgc
     4621 ccaaccgttt gatataaacg gtctacattg ctcatcccgc cccccaggac aatcacatcc
     4681 ggatcgagaa tattcacgac atgtgccagc gattttgcca gccgcagctc gtagcgacgc
     4741 aatgccagtt ccgctaccgg atcgctttct tcaaccaggc ggataatttc actgcctttc
     4801 agcgcatgtc cgctcaaacg acgataatct gtggcaaatc ccgtgcccga aataaaggtt
     4861 tcaatacaac cttgtttacc gcaataacaa gggacttcct cgcgataacg cagttcgtct
     4921 tcgtccatcc acggtagcgg attgtgtccc cactcgcctg ccgtgccatt gccgccgata
     4981 tgcgcccgcc cattgaatgc cacgcccgcg ccgcatcccg tgccgataat cacggcaaat
     5041 accgtctgtg ctcccgctgc cgcgccatct actgcttctg aaacggccag acagttggcg
     5101 tcatttgcca gccgcacttc ccgctgcaac ctcgcgctta agtctttatc gaatggctga
     5161 ccgttgagcc aggttgaatt ggcattctta accacaccgg tgtaaggcga aattgagcca
     5221 ggaatgccca tacctaccgt tccgcgctgc cctgtcgcct gctccgccat atcaaccaac
     5281 gtggcgatcg tttcaatagt ctgccggtaa tcatcacgcg gcgtgggcag acgatggcgg
     5341 tacaactgct cccctgcatc gcccagtgca atcacttcag ttttggtgcc gcctaaatcg
     5401 atacctatac gcacggtact ctccttattt ttttcaatat caatagcgta gagacggaca
     5461 accggattgg caatgcaagg ccgccgacaa ttcgttatca tgcccgctaa atttaacgac
     5521 aaggccgtgg aaattatcat gctgtggttc aaaaatttaa tggtttaccg tcttagccgc
     5581 gagatttcgc tgcgtgcaga agagatggaa aaacagctag cctcgatggc atttacccca
     5641 tgcggcagcc aggacatggc gaagatgggc tgggttcctc cgatgggatc gcacagcgat
     5701 gcgttaacgc acgttgccaa tggtcaaatt gttatctgcg cgcgcaaaga agaaaaaatc
     5761 ctcccgtctc cggtgattaa gcaggcgctg gaagcgaaaa tcgccaaact ggaagcggaa
     5821 caggcgcgta agctgaagaa aaccgaaaaa gattcgctga aagacgaagt gctgcactct
     5881 ctgttgccgc gtgctttcag ccgttttagc cagacaatga tgtggatcga cacggttaac
     5941 ggtttgatta tggtggactg cgccagtgcc aaaaaagcgg aagatacgct ggcactgctg
     6001 cgtaaaagcc tggggtcgtt accggttgta ccattgagca tggaaaaccc gattgaactg
     6061 acgttgaccg aatgggttcg ctccggtagt gcggcacagg gcttccagct gcttgatgaa
     6121 gccgagctga aatcgttgct ggaagatggc ggcgtgatcc gcgcgaagaa acaagatctg
     6181 accagcgaag agatcaccaa tcacattgaa gccggaaaag tggtgactaa actggcgctc
     6241 gactggcagc agcgcattca gtttgtgatg tgcgacgatg gttcgctcaa gcgtctgaag
     6301 ttctgcgacg agctgcgcga tcaaaacgaa gatatcgacc gtgaagattt cgcccagcgt
     6361 tttgatgccg atttcatcct gatgactggt gaactggcag cgttaattca aaacctgatt
     6421 gaaggattag gtggcgaagc acaacgttaa ttgctgattt tcctttaatg ccggatgcga
     6481 cgcctgccgc gtcttatccg gcgtacgaag ccacaccagg catataatta ttcgctacgg
     6541 cgggcaataa tttttagcgc agcaatatta tgcgttttat gctgtaactt gctccatgga
     6601 cgttgtgtca ttgtttttcc tcaagccagt gacgcgcttc actgcgaatt ttccgcattg
     6661 ccccggtttt cactaaatcc cgtaacagcg aaagatcggg ttgttcgaca aataagagta
     6721 atgatggatc ctcttttaac cacgtcgttt tcacatcggc agcgagctgt ggattattca
     6781 tagctagcga ccgatgccga gactcggtca atgtcgttaa caacgacgcc ggggtatgtg
     6841 cgttgcgtaa tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc
     6901 attcatcatc tgatttatct tttatcgtag ccagttccgc aacaatatct ggagatcttc
     6961 actgatattt atatcaacct tgatgaatta tgaatatacc ggaaattgaa aaaatcgcag
     7021 caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc ctcccgttca
     7081 aggggaaggc gaggaattac agatagcggc acagataaga ggtgggttcg gcaacttgca
     7141 gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac acctgccagt
     7201 cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatt tcttccggct
     7261 cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg cgaccagtgc
     7321 tgctgctgga aaagccgatt gatttcactt tgccggaaaa gtactcatta ctttgaagca
     7381 taaactggcc ctgtttggta gttgaacatc tacttcacta taggggccag aggcgctggc
     7441 tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata cgttagacag
     7501 caagacagga acatcgagct gtttttgcag taaatcgcga tgacgttggt gaaatcctaa
     7561 acaatccagc atgatgacat cagccccttt tgccagtaat tctttcccgg catcaatgat
     7621 tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcgatt tctgcaaaat
     7681 ttgccatttt tgcgcctgaa cgggcagcat ctcctcaacc ggaacgataa cccccacctg
     7741 atgatcttca acaatagagg aaaccagtgg aggcaatatt cgcgacggct caagaaagat
     7801 cgtattacgc gcagtcatac tactaatgtt tgctgtactc attaataaaa tgacgtcata
     7861 accctgatta tcgagcacct caaccacacc ttgcaggtct cgctccactt tacgacgcga
     7921 aacatgggcc agctggatgt catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg
     7981 cgcgtactcc gccatcactt cttcacgact taacttcccc agcaggctat gatgggaaat
     8041 attatcttcg tcaatgtatt ccgtcaggag cggcaaaacc tcctgcatag gtacaatgcc
     8101 gatggtcagg atcgccaacg acgcactcat tgttcatcca ccttactttt ctttcgtcgt
     8161 taattaccgg gcaagtgtga agcaccactg ctgacactta cttgtgctac aaatgacaaa
     8221 aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc cagtaatgga
     8281 ttgttattgt ttaatattct aattattaga aaaacatgaa ttatgaaaaa tgtgacgcag
     8341 atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcggtca ctctttgtca
     8401 gggtcctcat agcgtttttt cgcatccatc gcttcctgag ccgtcggatg ttcactgatc
     8461 aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggc
     8521 tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc
     8581 gttcttcctt atttcacggg atgaacgtta agtataggcg ctctaaaatc aacaattgat
     8641 cgtctgtgcc agggcgctac gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc
     8701 gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac
     8761 ttcttcgctc agcggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg
     8821 cagtcggtta accaggactg atactggcgc acacaaataa accacgatcc cgttattttg
     8881 cataaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga
     8941 tggcgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg cccactcttc
     9001 cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact gatcggtatc
     9061 gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc
     9121 ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat
     9181 gcggcgaaag ccggtgtcat gagaatagcg atcataccat caaactagta caatttcgat
     9241 tgtaaagaaa aaattccact taaagtgaaa atctcaatac atcccttact ataccaataa
     9301 atattcaaga atgaagtgta aataataaat tacatttagc cacgactacg ttgcacttcc
     9361 agccaccact tctccagctc cgcggcaaag gcctggcggt cacgttgtga aaggctatct
     9421 ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg
     9481 cgctcacgaa tggtcgctgg cgtgtaacgt tcgccgcgcg gattaagcgc cgctgcgcct
     9541 ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca
     9601 cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga
     9661 ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc
     9721 atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc
     9781 acccaaattg tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc
     9841 cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg
     9901 caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgcggcaata tgggaaaagc
     9961 cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatacac
    10021 cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc
    10081 ggcgcaagaa gtggcgcaaa tcgccgacat catttttgcc gccgttaaac ctggcatcat
    10141 gattaaagtg cttagcgaaa tcacctccag cctgaataaa gactctctgg tcgtttctat
    10201 tgctgcaggt gtcacgctcg accagcttgc ccgcgcgctg ggccatgacc ggaaaattat
    10261 tcgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa
    10321 cgcgctggta accccagaag ataccgctga tgtgctgaat attttccgct gctttggcga
    10381 agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc
    10441 agcctacgta tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc
    10501 acgcgcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct
    10561 ggaaacggga gaacatccgg gggcactgaa agatatggtc tgctcaccgg gaggcaccac
    10621 cattgaagcg gtacgcgtac tggaagagaa aggcttccgt gccgcagtga tcgaagcgat
    10681 gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgacttt cgccggacgt
    10741 caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtaaag cgccaaatct
    10801 gccgatttca accactcacg atagtgactc atttgtgggt tcagcggcgc aacccccaca
    10861 ctaatccgta aagttacctg tggcgtattc ggcaagcgta acgtgtttag cccttcacgc
    10921 acccgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat tactgcaaac
    10981 tcatcgccgc caaaccgacc aatcacatcg ctaccgcgca gggtaatttg taactgtcgg
    11041 gtaagcgcca caatcgcttc atcgcccaca tcatgacccc aggtatcgtt gatgctcttg
    11101 aaatggtcga tatcgataat cagtaacgtt gcatcgcgat tatgccgccg acagttatca
    11161 aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt catgccgtcg
    11221 cgggtactca tgacctgcaa cctgcgttta tgttccgcca gtttggttgc cgtctggtag
    11281 ctgacccagc caaacagcag aggataaacg acaataatgg gaagggagag ccaccattcc
    11341 agcggcgcac tattgaacga cacggtaatg cccgtaagct cgagggtgac aaggtaggaa
    11401 accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc
    11461 agacacataa tcatcaacat agcggtggaa ggcagcacgt ttacgcccat tacgcctacc
    11521 cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg
    11581 acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac
    11641 accagccacc accagcccgg cggcgggtgc gaaaccagcg ttgaagcaat cggtaagaac
    11701 atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg cagcccggac
    11761 cgctgatgtt cgttttgagg agttaaagga ggttcctccc catgcgccgc cgcttttttg
    11821 aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc ccaaattata
    11881 gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccactttcgt agctcacccg
    11941 ttagcactat caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat
    12001 catcaccttt cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct
    12061 gtggcgtcaa acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt
    12121 aggtcttacg agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca
    12181 tacgctgttg ttgtggggtt aaggttttct cggcagcgcc gacggtggtt aaaaaaacca
    12241 gaccaaaaag caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt
    12301 aaccatgagc gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac
    12361 cacccggcag cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc
    12421 atggtgtaga agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca
    12481 tacgccgcaa tacgcaactg actgccggta tgttcttgtg aatcctcttc agagttcccg
    12541 tagctcatca ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct
    12601 ttggtatccg gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc
    12661 gtgttaccct cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc
    12721 gtctcgccaa tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct
    12781 tcaacttgca ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc
    12841 atctgtgcca gggttggtac actgtcatta cgttgcggat taggcgtaca ggtgactgcg
    12901 ggcttatcga tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg
    12961 ccgtcagcaa acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt
    13021 gaggcagcat cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt
    13081 ccctgccatt caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta
    13141 acgtcggcac gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga
    13201 gcgttacccg gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca
    13261 tgtgccacca gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc
    13321 gccagacctg cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg
    13381 acgcccagcg cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc
    13441 gagtcggtga cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc
    13501 ccggtaagcg gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta
    13561 cgtgcggcag taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt
    13621 gcaggtttat cgctaagaga atcacgcaga gcggcggtat gatcacccgt taagcggcga
    13681 gcaccgccgg gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct
    13741 ggagtccggg cttttgtcac aggggtaaac agtaacgtta agagtgccag tgcaatagtg
    13801 ctttgtttca ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact
    13861 ataagtctcg gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgacg
    13921 aggatgcttc aataacttct ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat
    13981 gctggcatcg ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg
    14041 gtcattttgc gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg
    14101 cgcacacagt tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat
    14161 gagatttttc atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca
    14221 caacttcata gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat
    14281 tttttgtttg attaccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat
    14341 aggcttttaa ttatcaacct ttattataaa taaaagttat gaaatatatt ctcttcgtta
    14401 acttataatt aagagaaaaa aactcaccgc atggccattt aagccaggaa taaattagct
    14461 tcccgttcat catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca
    14521 gtacgttgca cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc
    14581 ccgttttgtc gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa
    14641 aaactgcggg taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa
    14701 tcggcaaaaa acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc
    14761 attgataaac aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac
    14821 gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa
    14881 catgagcatc agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt
    14941 ttcccgattg tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc
    15001 gccaatttac cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat
    15061 gtcaccaaac gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg
    15121 cgcgctaatc gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg
    15181 tttgtaaaac cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa
    15241 cagtacgcaa ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa
    15301 gggatcaaag gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc
    15361 acctgtggcg agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac
    15421 gaagatggcg cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc
    15481 cgggcgattg ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac
    15541 gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc
    15601 accaacatca gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg
    15661 atcacacgtt tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc
    15721 acaatgtaat attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg
    15781 cgacgacgaa taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg
    15841 gcataggtct gccaaccttt tgcttcaaat cccagtaacc ccaccacgcc attaagaatg
    15901 aatataaggc caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga
    15961 ttcatggcag ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt
    16021 ggtgtagaca acattttcgt aattgttaat aaatttaact taaggttgcc attttgtgac
    16081 tcacaacgaa gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata
    16141 ttgacaatct aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga
    16201 agcagatgac ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc
    16261 ggaaaatatc gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg
    16321 ttttgctttt gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat
    16381 gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc
    16441 ggcaccattt tcgggctgat cccgttttta gtaggctgcc tcatttttgc agtggtggcg
    16501 ctatggctgc actggcgaca tcgccgccag taagatttac gttgccagat acggcctaaa
    16561 acgccttatc tggcatgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt
    16621 cgtctacgcg cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca
    16681 gttgaatgcc aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt
    16741 gataaacttt cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact
    16801 gacggcgaat ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt
    16861 cggttttccc gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca
    16921 cgccgctggc cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag
    16981 cgttaaaatc agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat
    17041 ggtaagtacc aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt
    17101 ccattttcca cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg
    17161 gcccaccggt gcgcacagga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc
    17221 tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt
    17281 cgctataact tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag
    17341 gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg
    17401 gaacggccgg atctgcctga taatttgact cctgctgcgc caccgccagc accgagcaga
    17461 cgttttccag cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc
    17521 aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gccacatcta
    17581 tcgctttttc gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca
    17641 gaaccgccaa caatgacagc gaagatagag gattgacgcg cgacattact tctccttttt
    17701 agctcaaggt atgggttact tcctgaattt tgtcaccatc cagttcatgt tcaaagctgc
    17761 gcagacgttt gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact
    17821 ggaaagcacc gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa
    17881 tcgtaccggc aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca
    17941 aatagagaat gcgggcgatg ttgaaataca tatagtgaaa atagaggcgg aaatagtttt
    18001 tccgcacggc gctaaacagc tcgcgtaccg taggcggcgt cgcacgcgtg gcatcgtctt
    18061 caccataaac cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc
    18121 ccggcagttt gatccctacc actgccagca atccggtccc catcagcgac cagacgatag
    18181 cggcaatcac cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgtgg
    18241 agagcgttac cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga
    18301 cgcccatatt ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg
    18361 ccccttcgat atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac
    18421 gccagcggaa cacgtagtga ctgacaaaga agttgttcag cacactgatc accacagcga
    18481 tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat
    18541 gcggcgaact tagcgcggtt tgaatcagat catagaacgg tgcataccag gcgttgacgg
    18601 cgaccccgac ttccaccaaa aaccaggtga cgaagatgat cagtgcagta ccgagtattg
    18661 accagtattg ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc
    18721 aaacaatgta gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa
    18781 tcgggatctg cccggaagcg ccagtgatac gcgccaccca ctctccccca ccggcttgcc
    18841 agaagataac ggcaatcaat gcccaaacaa aggccgagag aaaaaacgct cccggctttg
    18901 ggaaaaaaga cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt
    18961 gtattaaccg catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat
    19021 agcaaggcgc ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc
    19081 aaagattgtt tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag
    19141 caggaaaaag atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg
    19201 ccctttgcaa tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg
    19261 gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac
    19321 ctccgtccat gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg
    19381 ttatgccgat catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga
    19441 cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga cctggtaata acgttcgccc
    19501 gcagctggat tcggtcgtgc gtattgcttc cctcaccaag ctaatgacca gtgaaatgct
    19561 ggtgaaattg ctcgaccagg ggaccgtgaa gttaaacgat ccgctaagta aatatgctcc
    19621 gccaggcgca cgcgtgccaa cctacaatgg gacgcccatt accctagtga atctggcaac
    19681 ccataccagc gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt
    19741 ctggccaacg cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc
    19801 aggcagtcag gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa
    19861 tgcgtccggc aagccttata cccagttgtt tgaagagcag attacccgtc cattggggat
    19921 gaaagacacc acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg
    19981 cggtgccagt ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac
    20041 gcctggcgat atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag
    20101 taaccaggca gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat
    20161 tggcatggat gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc
    20221 gaaagagggt cgtccgggga ttattcagaa gacaggcggt ggtggcggtt tcattaccta
    20281 tatggcgatg atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt
    20341 gacgcgcttt aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa
    20401 taaaccgttg gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga
    20461 ccagtttgcc tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg
    20521 cgacgacaga acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg
    20581 atcgcaattc ttcattccag tcaatcattg ataataatgt tgcgcggatt tgttcgtagg
    20641 aattatgccc aatgagctgc acgtcgcgta attccagaat tctattttgc caggctaacc
    20701 agtaaaaagc gtctcgccag agttgatttt gttcaataag cgtaatggtt tgtttggctg
    20761 gtagatgata tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca
    20821 ttaaaccatc agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt
    20881 cttctctacg cagagagata acgccctcta aaataacgaa agtatcttcg ttattgacat
    20941 cactggaaaa tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca
    21001 aacatttatc gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt
    21061 cctttgcagt aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagaat
    21121 ttcttcggag tgaagaatat taccaggtat atttaacacc cacgttcgcg gaccagtctt
    21181 gatctacgtc accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga
    21241 agctgaactg agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg
    21301 aatcaccgtt cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt
    21361 acggagtcag agcctggtct tcgctgtagg tgaaggtata acctgcatct acacccagtt
    21421 cataacgcat gctgtcgtaa gactgaccgt caactttcat gtcgttgctc agctggtagt
    21481 catcaccaga ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac
    21541 ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca agcgtcggag ttggtgctac
    21601 cgtcaacgta agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtaac
    21661 tcaagctacc atcaacaaag acgttgttcg cgaagtgagc agaagagtag atgtaggcag
    21721 tctggctgtc ttgatccacc tgaccagaac ggtcattcat gtcaccttta gcgaagcctg
    21781 cagccgcacc gacgatccac ttagcgttgt taccgtcaat tttggtatca acaccgacca
    21841 tgatgccgtt aacatcctga tcatagttga tggtgccgtt gtcgccgttg aagttaccac
    21901 cgaagtagct tacccatgcg ccgccgttat cagccaggcc atgacgagag ttggtcagac
    21961 gagtaccaac ggtgtcttgt tccaggttcc agatattggt gttcgcagac gggatgctca
    22021 gcgccatgtt agcgtagtcg gtcagctcca tctgttgcag aacaacggtg ttaccgcgct
    22081 gttcagcctg ataggtgtat gcacccaggt cagctttgtt agcagcagag aaggtcgcgt
    22141 tggtgttgac gtcgtttacg tagataattt ctttatcttt gtaatcagcg atagcgccag
    22201 cgcctgttgc gttgtcgata cgaactttgt agttaccggt tgcagcaaca acgttgtcaa
    22261 cagagctgtt atccagttcg tcgccgttgt tcatgtcacc gttaccgttg atagtcagat
    22321 gaccatcaga gttcatagca acaataccgt agccgtagtt agacttggaa gtatcccagg
    22381 tacggtcgtt caccaaatca gcgttcagaa cataatcgct gctgtggata tcgaatacac
    22441 cagacactac gttgccgtta tggtcagcga tgttgccgct ggtcagttcc aaagtatcag
    22501 tctggaacgg attatcatcg aactggtcta cattaatatc cagtaaacca ccgttagtca
    22561 cagttattgt atttgctaac agcgacggag tgtaataacc attatagata cttgaattgt
    22621 catcactaga cagcgctaca gtactgtagc tatcaactgt cagatgatca gttccaacat
    22681 aaccttcatc tccaatgttc aaagaagaac cattggtcag agcgatgcta tcagaaatca
    22741 agctagattc agacacatta acctgagagc cattattcac agctaaagta tcaatgtttg
    22801 aagcgttaac ggtattccat tcagaaccat tattcagagt gacgttaaac acaccgctct
    22861 ggaatacttg atcatcaatc gtacgacccc aagcatttgc ttctgttaca acgcccggat
    22921 acaggctatt acctgtaaca tcataccagt cagtcgaagc agtagattca tcatcaacat
    22981 ttgctgatgt agtagctgag cctacccact tagaaccatt atccagagtg atattcagtt
    23041 catcaacatt aagtgagtca tcagcccagc caccattggt gtccagtacg ccatcggcat
    23101 tactgtcatg accttgagga tagaagtttg cgttgaaagt acttgcgaaa acaacatcac
    23161 ccagcagagt agaattgctg aaataaacat tgtttttcat ggagtaattt gaagccgcac
    23221 tatcactgaa atacagagct acatcattag cttcgccgtt accagagtaa tcactaggtt
    23281 ccgcagagtt accatagaaa ccactgtctt ccagagtagt tacagaacct gaagtcactg
    23341 tggaatcggt aactgagata ttgttatccc aagtatgaga ttcagtagtg taagtattgt
    23401 ttacggtatt accctgagtc aggctgatac cagcaacgcg ggagttgtta gaaatattga
    23461 tgttactttc gacatcaaga ttcactgcca gcccaaggcc aacaccaccg tttgcaaaca
    23521 aaccggcgta ttcttcacct tcaccagctg gggtatggaa ggtttcgtta gtatatttgg
    23581 taacgtcacc gtccagatat gaatcagtga agtataaacc ttcataatcg tcatcaattg
    23641 tagaattagc aatatttaaa gtgaatacat caccatcatg ccaattatca tcaattacac
    23701 gatcagtacc gtattcagag aactgataga cataatgacc atcactgctt tcacccatag
    23761 gcagcattga agtgattgaa ccatgaatca gggagttgct gatgttcaac tggtttgttg
    23821 ttccatcagt aaagtcatga ttcaggtaat atgtagagat agtcccgtta acagtgtcac
    23881 cattaaaggt ggtatcgaaa tcaccgtttg tgttgttata gtagacaaag ccactataat
    23941 tcacatagtt accatctaca gtacccggtg catccatatt gctccagtca agagcggtag
    24001 ttggattgtc gtaataagtc caggtgtcat aagtagttgc attagcctga gaggtgatag
    24061 ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag gagtgcattt
    24121 gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca tagaaaacat
    24181 attatgtttc gcgctaatga ttatgctgcg ttacagacga atggttcaat tattctttcc
    24241 cctaagtccg aatgtggaca tatataacct atggagaaaa acacgcatca aattgataaa
    24301 agggcgatat ttcaacatgt tgcagataat gttatttttg atacatacgc gttataaata
    24361 tcaaaaacac aaaccagata aatatcttat caatatacaa caaagtttga attattcaat
    24421 ttatagcagc acaaccaaac gccaaaaaat aataaccaag agctataaat aaagattaaa
    24481 aatattttca gtgaatatga aattattgct atttctatta caaagatgtc ctggtctctc
    24541 tttcattctc ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca
    24601 ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac
    24661 gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta
    24721 gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg
    24781 ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac
    24841 gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac cataccgatg
    24901 aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca
    24961 agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc
    25021 acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcaact ctggaaaatt
    25081 taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg
    25141 cgatggacgg ccaggtacag gcgattcgcc aggcgctgga cgctgcggga tttaaagata
    25201 cggcgattat gtcgtattcg accaagttcg cctcctcctt ttatggcccg ttccgtgaag
    25261 ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca atgaaccgcc
    25321 gcgaggcgat tcgtgaatca ctgctggatg aagcccaggg cgcagactgc ctgatggtta
    25381 aacctgctgg agcatacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga
    25441 ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg
    25501 ctatagatga agagaaagtc gtgctcgaaa gcttaggttc gattaagcgt gcgggtgcgg
    25561 atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt
    25621 tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta
    25681 accctctccc caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac
    25741 ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag gtaatcggca
    25801 ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg ttgccagcgc
    25861 caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg
    25921 ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc
    25981 tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg
    26041 accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac
    26101 gatttacgga aatcatgctc cgcacgcagc ccgctcagca gctggcggaa gggaacagag
    26161 agcgcctcat aggccgcaat accgctggtc cagagcgtat caccgccggt cgaaggtaac
    26221 tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc
    26281 cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc
    26341 ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg
    26401 cgttgctgct gcggcgtaat agtttgatcg cgcagaaaca ccacctgatg gcgcagcacc
    26461 gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa
    26521 atttgtgcgc cgatatacgg ccccagcggg gtaatgctca gacgttcact cattgtactt
    26581 ctccatgcca gggcgtcaga cggcgctgta acgcgcgcaa acccagttct aaaagaaagg
    26641 cgataatcgc aatcaccgcg attcccgcca gcaccacgtc agtcgcgaga aattcacccg
    26701 ctgactgaac cataaatcct aaaccgcgcg tcgcggcaat cagctccgcc gctaccagcg
    26761 tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg
    26821 gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa
    26881 tgcgaacctg ctgcgcgctt ttcacccccg ccagcgccga catcgccacc ggtgcaaaaa
    26941 tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag atcaccatca
    27001 gcggcaaata agccagcggc ggcacgggac gataaagttc gattaccgga tccagaatgc
    27061 cgcgcaccgt cgggcttggc cccatggcga tcccgaccgg aataccgatc accaccgccg
    27121 cgagtagcgc cagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt
    27181 ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg
    27241 gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa
    27301 ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc
    27361 gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa
    27421 tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg
    27481 gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct ccagcacacg
    27541 gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc
    27601 gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg
    27661 catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg
    27721 gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg
    27781 ccagatgtag cgtttttctg cgccttccag ccccactttt ttcagcatct ggtgcgcgat
    27841 ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg
    27901 tacattgcgc cacggtagta gcccttcatt ctgaaaaact acgccacgct ctgctcccgg
    27961 tccctcaata cgcttacccg ccagtagaat gctgccatgc tgataaggca caaaaccggc
    28021 aatcagattc agcaacgtgg ttttaccgca gccggacggc cccagcacca ccagtagctc
    28081 gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcgta
    28141 aagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta
    28201 ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt
    28261 gtcgatgatc gctttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag
    28321 ataggtattc cccttcacca gccccggaac gtcaccttca ggcacgccgc ttaaacgcgc
    28381 cagtttgctg atgttttccg gctgtttcag ccacacgtct gggttagcaa tgtacggttg
    28441 ctgagcatcg atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcata
    28501 atctttgcgc accacccaga cgtccagcgt tggcgcgccc cactgcccga cctgttcaga
    28561 atcggtcaac accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc
    28621 accatcaata tctccccgct gccaggcagt gataatcgcg ggcggctgca ggttcacaat
    28681 ctccacttgc ccgggtttaa tgccccagtg tttcagcgcc gccagcaggc tgtagtgggt
    28741 ggtggagata aacggtacgg cgatgcgttt gccaatcaga tcttccggtt tgctgatagt
    28801 tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat
    28861 cggcacctgt tggctggctg caaccgctaa cgggctggaa ccgaggttgc cgatttgcac
    28921 atcgcctgaa gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac
    28981 gctcgccccg ctctctttag caaaggtgtt gtcggcctga gccactttcg ccggttcggc
    29041 tgaggtttga taggccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag
    29101 tgcggctaga agtgtgttac gcgatgaaat tgccatgatt gtctgcgccc ctgtcttgtt
    29161 atgggagcag tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga
    29221 tgattttatt cttagcaaaa aaacggctaa ggaaagatta aatatgcaat tactccgttc
    29281 taacaattgg gaaaattact acacttcatt ttagtgatgc ataaacaacc acattacgag
    29341 atttattact tcattatttc tatcaatatc aatacatata ataaaactat tatattcgca
    29401 ttgtatatat cactgtatca ttctcaatca ctcgcatatt acattcacat aatgtatata
    29461 aacaatgctt gttcacagaa catatttttt aaatgcaatt aattgacaaa ctaaatcaca
    29521 tctaaataca atgtataaaa caaaataact atacatacca aacatacaaa cacttaacat
    29581 atgaattgca tagaaacatt acccagtcga acctccgggt ctgaaagccc ttgcttttca
    29641 gttattagca acatttaaca tttataaatt aacatctggc tgcgccagag attacgtctc
    29701 tgagtgcatc gttgtgcttg tagtataata aataaaatgg agtgtcatta agtggataag
    29761 gttttagatt cagccctcct ttcttcggca aataaaagaa agggtatcct tgctattggc
    29821 gcacatcctg acgatataga attaggctgt ggcgcatcgc ttgctcgtct tgcgcaaaaa
    29881 ggaatttata tcgcagccgt ggtgatgact accggcaact ctggcacaga tggaataatc
    29941 gatcgccatg aagaatcgcg caacgcccta aagatattag ggtgccacca aactattcat
    30001 cttaattttg ctgacacccg cgctcattta cagctcaatg atatgatttc cgccctggaa
    30061 gacatcatta aaaatcaaat tccttctgat gttgaaatca tgcgggtata taccatgcat
    30121 gatgccgacc gccatcagga tcatctcgct gtttatcaag cttcaatggt tgcctgccgc
    30181 actattccac aaattctcgg ctacgaaacc ccaagtacct ggctttcatt tatgcctcag
    30241 gtttttgaat ccgttaaaga agaatatttc acagtcaaac ttgctgcatt aaaaaaacat
    30301 aaaagccagg aacgacgcga ttatatgcgt catgatcgcc tgcgtgcagt tgcacaattt
    30361 cgcgggcaac aggtcaatag cgatctgggt gaaggctttg taattcataa aatgattctt
    30421 tgagcaaatt gcatgaaaac ctggatattt atctgtatgt ccatagcaat gttgctatgg
    30481 tttttaagta cgctaagacg taaacccagt caaaagaaag gctgtattga cgccattata
    30541 cctgcgtata acgaaggccc gtgtctggcg cagtcactgg ataatctact gcgtaaccct
    30601 tatttttgcc gggtaatttg cgttaacgac ggctccacgg acaataccga agcggtcatg
    30661 gcggaagtca aacgcaaatg gggcgaccgc tttgttgccg tcacgcaaaa aaataccggt
    30721 aaaggtggtg cgctgatgaa tggcctcaat tatgccacct gcgaccaggt ttttttaagt
    30781 gatgccgaca cctatgttcc gcccgatcaa gacggaatgg gctatatgct ggcagaaatt
    30841 gagcgcggtg ctgatgccgt aggcggcatt ccctctactg cgttgaaagg cgcggggctg
    30901 ttaccgcaca tccgcgcgac cgtaaagttg ccgatgattg ttatgaagcg cacgctacag
    30961 cagctcctgg gcggcgcacc gtttattatc agcggtgcct gcggaatgtt ccgcactgat
    31021 gtattgcgta agttcggttt ctcagatcgt actaaagtcg aagaccttga tctcacctgg
    31081 acattagtgg caaacggcta ccgtattcgg caggcgaatc gctgcatcgt atacccacag
    31141 gaatgcaaca gcccgcgtga ggagtggcgt cgctggcggc gttggattgt gggatacgcg
    31201 gtctgtatgc gcctgcataa aagactttta tttagccgct tcggtatctt cagtatattt
    31261 cctatgctgt tggttgtgat ttatggcgtt gggatttatc tcactacctg gtttaatgaa
    31321 ttcatcacca ccgggccgca tggtgtggtg ttggcaatgt ttccgcttat ctggatcggc
    31381 gtagtttgtg ttattggtgc ttttagcgcc tggtttcatc gttgctggtt gttggtgcct
    31441 ttagcgccgc tttccgttgt gtatgtatta ttagcttatg ccatctggat tatttatgga
    31501 cttattgcct tttttactgg acgcgaacct cagcgcgaca aacccacccg ctattccgca
    31561 ctggtggaag cgtcaaccgc ttattcccaa ccttctgtca caggaactga aaaactatct
    31621 gaagcttaat gttaaagaga aaaatattca gattgcagac caggtaatta ttgatgaaac
    31681 tgcaggtgaa gttgttatcg gcgcgaatac ccgtatttgt catggtgccg ttattcaggg
    31741 tccggtagtg attggcgcaa actgcctgat aggtaattat gcgtttattc gtcctggcac
    31801 aataatcagc aatggtgtaa aaattggttt tgccaccgaa attaaaaatg cggttattga
    31861 agcggaagca acgattggtc cgcaatgttt tattgccgac tcggtagttg caaaccaggc
    31921 atatttgggc gcacaagtac gtaccagtaa tcatcgtctg gatgaacagc ctgtgtctgt
    31981 tcgaactcca gagggaatta tcgctaccgg atgcgataaa ttaggttgtt atatcgggca
    32041 gcgttcacgc cttggcgtac aagttataat tttgcctggg cgaattattt ccccgaacac
    32101 gcaacttggc ccgcgcgtga ttgtagaacg taatttacct gccggaactt attcactccg
    32161 acaagaactt atccgtacag gagattaaaa tgataaaacg gacgttatta gcggcggcca
    32221 tttttagcgc attgcccgct tatgccgggt taacttccat taccgcgggc tacgatttta
    32281 ccgattattc tggcgatcat ggcaaccgta atttagcgta tgctgaactg gtggcgaaag
    32341 ttgaaaacgc aacgctgctt tttaatcttt cccaggggcg tcgtgattat gaaactgaac
    32401 atttcaatgc cactcgcggt cagggtgcgg tctggtataa atggaataac tggctgacaa
    32461 cccgaacggg tattgccttt gcggataata cgccggtctt tgcccgccag gattttcgtc
    32521 aggatattaa cctggccctg ttgccaaaaa cgcttttcac gaccggttat cgctacacta
    32581 aatattacga tgatgtcgaa gtcgatgcct ggcaaggcgg cgtatcactc tatactggcc
    32641 cggtgatcac cagctaccgc tatacccatt atgactccag cgatgcaggt ggtagttata
    32701 gcaatatgat ttccgtgcgt ctgaatgacc cgcgcggcac tggttatacg caactatggc
    32761 taagccgcgg aacaggcgct tacacctatg actggacgcc agaaacacgc tacggcagca
    32821 tgaagagcgt cagtttgcaa cgtattcaac cgctgactga gcaacttaat ctcgggctga
    32881 cggcaggtaa agtgtggtac gacaccccaa ccgatgatta taacggtctg caacttgcag
    32941 cccatctgac ctggaaattc tgattccttc tgccgcccgc tatccggggc ggccttccct
    33001 gccgattagc cccccctttc ctctttgttt tccgaccaca ttcaccggat aaattttatt
    33061 ctccagtgtt atatactata ggggggtatg cattgacata tagcataccc ccctatagta
    33121 tattgcgtgc agatgatgag gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc
    33181 cttactcgag ttcgtcgtat tcgagggcag attgatgctc tggaacggtc gctggagggt
    33241 gatgccgaat gccgtgccat actccaacag atcgctgccg ttcggggcgc ggctaacggg
    33301 ctgatggcag aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa tgactgctac
    33361 agccgcgaag tcagccaatc cgttgacgac actattgaac tggttcgagc ctatcttaaa
    33421 tagctgaatc tattaccata ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc
    33481 atttgctccc ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg
    33541 tgaagtgctg attaaagtca cccataccgg cgtttgccat accgacgcat ttaccctctc
    33601 cggggatgac ccggaaggtg tattcccggt ggttctcggt cacgaagggg ccggcgttgt
    33661 ggttgaagtc ggtgaaggcg taaccagcgt caaacctggc gaccatgtga tcccgcttta
    33721 caccgcggag tgcggcgagt gtgagttctg tcgttctggc aaaactaacc tctgtgttgc
    33781 ggttcgcgaa acccagggta aaggcttgat gccagacggc accacccgtt tttcttacaa
    33841 cgggcagccg ctttatcact acatggggtg ctctacattc agtgaataca ccgtggtcgc
    33901 ggaagtgtct ctggccaaaa ttaatccaga agcaaaccat gaacacgtct gtctgctggg
    33961 ctgtggcgtg accaccggta ttggcgcggt gcacaacaca gctaaagtcc agccaggtga
    34021 ttctgttgcc gtgtttggtc ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca
    34081 ggcgaaagcg ggacggatta tcgctatcga taccaacccg aagaaattcg atctggcgcg
    34141 tcgcttcggt gctaccgact gcattaaccc gaatgactac gacaaaccga ttaaagatgt
    34201 cctgctggat atcaacaaat ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa
    34261 cgtgatgcgt gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg
    34321 ggtggcaggt tctggtcagg aaatctccac ccgtccattc cagttggtca ctggtcgcgt
    34381 atggaaaggt tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga
    34441 agatgcgatg aaaggtgata tcgatctgga accgtttgtc acgcatacca tgagccttga
    34501 tgaaattaat gacgccttcg acctgatgca tgaaggcaaa tccattcgaa ccgtaattcg
    34561 ttactgattt cccgcaggta taccccgtcc acttcagacg gggttcttaa tactctcccc
    34621 gggcagccgt ccgggggatt aaccgtgaga taatgactga tggaactcat tgaaaaacat
    34681 gccagctttg gcggctggca aaatgtgtat cggcattatt cccaatcact gaaatgtgaa
    34741 atgaatgtcg gcgtctatct cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac
    34801 tggctttcgg gcctgacctg caacgagcag aatttcatta ctaaatcggg gatgcagcgt
    34861 tacgcggctg aacacaacat tattgttgtt gcgccggaca ccagtccgcg aggcagtcat
    34921 gtcgcagatg ccgaccgtta cgatctcggg caaggtgccg ggttttacct gaacgcaacg
    34981 caagcgccgt ggaatgaaca ttacaaaatg tatgactata tccgcaacga gctgccggat
    35041 ttagtgatgc aacattttcc ggcaacgacc agaaagtcta tctctggtca ttctatgggc
    35101 gggctgggcg cgctggtgct ggcgttacgt aacccagatg aatatgtcag cgtctcggcg
    35161 ttttcgccca ttgtctcccc atcgcaagta ccgtggggac agcaagcctt tgccgcatat
    35221 cttggtgaaa ataaagatgc ctggttgaat tacgatccgg tgagtcttat ttcacaaggt
    35281 caacgcgttg cggaaatcat ggttgatcag gggttgagtg atgattttta cgcggaacag
    35341 ctgcggactc caaatcttga aaagatctgc caggagatga atatcaagac gttaatccgt
    35401 tatcacgagg gttacgacca cagctattat tttgtctcca gttttattgg cgagcatatt
    35461 gcctaccacg ccaataaact gaatatgcgt tgataatagt gcacgactgc cggatgcggc
    35521 gtgaacgcct tatccgccta catttcgccc gcaggccgta ggcatgataa gacgcgcata
    35581 gcgtcgcatc aggcatctgc gcacgactta tctatgttta ccacatcaaa tcatcaggca
    35641 ctttgaagtc ggcatatggg tcgtcttcat cctgctcttc ggcactgagc gcgctgtgca
    35701 acacaatact gctggcatca cgctgagcaa ttttatcggc aacgctggcg ggaataatgg
    35761 cgtattcacc ttcgctattg ttatcgacca acaagcgggc aatcgccaga cgaccattaa
    35821 tcaactgcgc ctgtgtgagc ttatcgacaa aaatcttctt aatcagattg ccgtcagtga
    35881 agttaaaacc aatatcacca ttggcaatgg tgattcggtt catttcaata agctgcttca
    35941 cctgggcttt atattctttc gccaacgccg cttgtttttg ctgttcgcta agctgtttat
    36001 cacgctcaag ctgtgccttt ttattttctt ctaccgccgc ccgagcttca cgcgcctgaa
    36061 cgcgcgattt tttcgccgtt ctctcgacct tcgccgcttt tttgctggtg actaatcctg
    36121 ctttgagcaa ctgctcttgt aaggtaagtt ttgccatctt tgtttccaaa cccgctgaat
    36181 aatttttagc gagtatacct gtaaacgccg tgggtgtatt gggggcaagt ccctttcgcc
    36241 cctgctaaat aaggcgcgtc aggcatcggc acacggctgc attcttgatc tccgactcat
    36301 caaaataaac accgccaacc cagcaacaag aatacccggt gcagaagcgg ccattacgcc
    36361 gaccgtgcca gtgcctaatg ccagcatttt cccggccagt aacggaccgc tcatagcccc
    36421 cagacgccct accgccacgg ctgttcccac acctgttgcg cggatctgcg aactgtaaaa
    36481 caacggtgcc agggcataca aaacgctttg cccacctgtc gcaaacaacc ccgcgacaaa
    36541 tcccgccagc aacataccgt taaatgacga caccgttcca agcgccagca gcgaagctaa
    36601 catgccgcta taaatcagta gcgacatggt tactggacgc agcttatcca tcaatgcgcc
    36661 caacattaac gtcccgcttg ccgcccccat ttgcagggca aacatcaccc ctgccgcctg
    36721 cgatggctgg aatccttgct ccaccaaaag tagcggtagc cagttgatca acatataaac
    36781 caccagcaga gtgaagaaat aacacaacca cagcagcagc gtcgcggttg ccgtttctgg
    36841 cgcaaataag gcacgcagtg gtggcgcagc ctgtttttcg ccagcgaaaa ccgctgactc
    36901 cggcagccag cgcattaata gcggcaccag aatcaacggc accacaccac ctacccaaaa
    36961 caccgtttgc catgctaagt ttgcccccgc gaaacccagt gtcgccgcca gcgccgcgcc
    37021 aatgggaaca ccgcaataca tcaggctcac tgccgtccca cgaaaacgtg gacccgcggc
    37081 ttcagacgtc agggcgataa gattcggcaa cgccgccccc agcccgacac cggtcatcag
    37141 ccgcgcaaag accagtgagg ggaaatccca agcaatcgcc gttgccagtg agaacaaacc
    37201 aaacagcgca actgagccaa tcaaaatact cttacgacca taacggtctg ccagcatccc
    37261 gccaaccaac gcgccgggta gcaaaccgag tattccggcg ctaaatatcc agcccatttg
    37321 cattttatcg agtgcgaaag cctgggcgat gccacccgcc gcaatgccag ccgcctgaag
    37381 atccagccct tccatcagag cgaccaaaaa acaaagcccg atggtcagca tcaggcggga
    37441 tgaagatgat gaaggggtac gagtcgacat aagatgaacc tcgttgcgag tgcaggtgaa
    37501 tcgcgactga tacattgcca gccgcgtaat cagaactatt taatgactgc ctacgggcga
    37561 aatgtaggcc ggacaagacg tttatcgcat ccggcagtcg tgtgcagatg cctgatgcga
    37621 cgctaacgcg tcttatcagg cctacaaatt ctcacgccac ccgtaggccg gataaggtgt
    37681 ttacgccgca tccggcagtc gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc
    37741 atcattattt gttgttgcgc agatccagcg ccacgtcaac gatcatatcc tcctggccgc
    37801 caaccatccg gcgtttgccc agctcaacga gaatatccac cgcacttaag ccataacgcg
    37861 ccgccgccgt ttcacagtga cgcaggaagc tcgagtaaac accagcgtat cccagcgcca
    37921 gcgtttcgcg atcgactcgt accggtcgat cctgcaacgg acgcaccagg tcgtcagcgg
    37981 catccattaa cgcatagaga tcggtcccat gctgccagcc cagtttatcc gcggcggcaa
    38041 taaacacttc cagcggtgcg ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga
    38101 ttcggtcgca gccctcttcc accgccgcga tagagttcgc cacgccaaga ctcaggttat
    38161 ggtgagcgtg catgccagtt tgcgtttctg gtttcagctc tgctttcagg gcgcggaaac
    38221 ggtcacggat atcgctcatg ttcatcgcac cgccagaatc caccacataa atacaggtcg
    38281 caccgtagcc ttccatcagc tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc
    38341 tcatcatcag aaaaccaacg gtgtccattc cgagctcgcg ggcatactga atatgctggg
    38401 cggaaacatc agcttcggta cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc
    38461 aggcattttt cagatcgtga atagtgccga ttcctggcag caacaacgtc gcgattttgg
    38521 cgtgcttcac cacatccgcc gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac
    38581 catagttaaa gctggaaccc tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc
    38641 gggcatcgtc cagtgctttg gcaatctggc gaacgttttc cagcgaatac tgatgacgaa
    38701 tggcgtgcat accgtcacgc aacgtgacgt ccgagatata aagtttttta ccgttcatgc
    38761 cgcttctcct gccttgcgcg ccagtgactg ggccattttt tccgctgtcg ccagcgcact
    38821 ggaagtcata atgtcgaggt tgcccgcata ggcaggcaga taatgcgctg cgccttcgac
    38881 ttccagccag accgctgttt tcagtccgga gaattgcccc acgcccggta aattgaccgg
    38941 tttatcctgc gggataactt caaactgcac gcgctgtttc aggcgataac ccggtacgta
    39001 agcctgcacc gcctcagcca tttcattgat tgaggcttcg atatcatctt gtgaagcttc
    39061 gtcgctcaat acatacaccg tgtcacgcat catcaacggt ggctctgctg ggttaagcac
    39121 aataatcgcc ttcccttttg ccgcgccgcc caccacttca atggctcggg aagtggtttc
    39181 cgtaaattca tcgatattgg cacgcgtgcc aggtccggca gatttactgg cgatagaagc
    39241 gataatttcg gcgtaatgaa cacgcgccac gcgtgaaact gccgccacca ttggaatggt
    39301 ggcctggccg ccgcaggtga ccatgttgac gttcagttga tcgacgttcg cctcgaggtt
    39361 aaccaccggc acgcagtaag ggccgatggc agcaggcgtc aggtcaatta agcgaatatc
    39421 cggtttcgct tcgcgtaaag cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc
    39481 aaatacaatg tcgatatcag caaattcagg catgttcatc agcccgatca ccccttcatg
    39541 ggtggtggcg acgcccatac gtctggcacg cgccagaccg tcggactgag gatcaatgcc
    39601 gaccatcacc gccatctcca gatgctgacc gtgacgcaaa attttaatca tcagatcggt
    39661 gccaatgttg ccagaaccga taatggcgac tttacgctta ctcatgacag acttcctttt
    39721 ggggctgcgc ttgaaaatgt cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa
    39781 cgatcgcccg cattcactgc caccatcgga cctaatgccc cggtaagaat gatatctccg
    39841 gcgcgcagcg gttcacccag gctggccatt ttgcgtgcca gccagacggc ggcattaagc
    39901 ggatgtccca ggcattcgct gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc
    39961 ttcatggcgc agtttttcag gtctaacccc gccggacgtt gcgccggacc gccgatgaca
    40021 tacaccccgc aggaagcgtt atctgccacg gtatcgacaa actgaatcga ccagtcgcga
    40081 atgcggctac ccaccacctc cagcgccgga agtacccatt caatggcgtt atacagttcg
    40141 tcgaaggtga tatcggttgc gggcaaatcg cggttcaaca ccagtgcgat ctccgcttca
    40201 atgcgggctt gcagaacacg ggaaaaagga atgatttcgt tatcgccata acacatgtcg
    40261 gcaaataatg tcccaaaatc cggttgatca acgcccagtt gttgctgcac tttcggatgc
    40321 gtcaggccca ctttacgccc taccacgcga cgcccctgcg caacgtcata ttgcacattt
    40381 atgtgctgaa tggcgtaggc cgcttcagcg ttatcgatac caatcagatc gcgcagcggc
    40441 gcaatcgctt cgccctgctc tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta
    40501 tgcttcgtca tgaccattcc ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa
    40561 gcgtcggcat gttcccactg cgcccagtga ccacagtcgc ggaagatatg cagttcagaa
    40621 ccggcaatgc cggacagcag acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc
    40681 ccccagacaa tcagggtttg cgctttgatt tccgccagac gtgggccaaa atccgggaac
    40741 tgtttcggat tagcttccag gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc
    40801 atattattca ggcgcgcttc aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag
    40861 atatccatca tcagcttcag gttttcgata gtcggctgac gataaagctg attcagtcgc
    40921 ttaatacctt cggttggcat cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc
    40981 agcaccagtt tgccgacgcg ctccggccag ttaagggtga acgccataga actatggccg
    41041 cccatcgagt tgcccagcag gtggattttg gcgatatcca gttgatccac cacgcttttc
    41101 aggattcgtg cattaagatc cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc
    41161 caacccggac aatccagcag gattacccga tagcccgcct ctaccagcgg atcgatattg
    41221 cggctgaagt tcgcccagcc agtagcaccc gggccggaac cgtgcagcag gacaacggtt
    41281 tcgtcgcctt gtccgcagtc attaaaatgg atgcgcagcg ttttacccgc ttcttctaca
    41341 ttcagaaaac ggctggtggc ggcttcggtt tgtggctgat aactcatcat cttctcctgc
    41401 atattcagtt ctctgttctg gcgcttaacg agccaaatcc ggcaatccac tccgggattg
    41461 ggcggtaata acgcccttcg ctacgccagt tgccaaatgc agaaatagcg gcaaaagcgg
    41521 cgacccaggt tttgatttca tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac
    41581 tgacggcatc cagttcctgt atgcgtccct gctccagcaa agtcatgaac tggttatccc
    41641 aaatcgggtt gagcggatgc agagttctct gatcctcaac aaacttctca gcggcgctaa
    41701 tcacccgttg ctgacgcaat tcgcgctcac tggcgggtaa atctttcccg ctccccaaca
    41761 gacggtcgcg catatgggca tcggctttcg ccagttcggg caccggcggc tgatgggaaa
    41821 gcccaccgga acccaggaac agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg
    41881 cttcacccaa catgcgggta cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga
    41941 acacaggcag aactggcacc ttatccagcc cgccgagcag gaactccagt ggctgggcga
    42001 acccgtggtc cacctgcata cagtaagaaa cggcaagatc gatcccgctc ttcatgacgg
    42061 catgcgcaca ggcctccgcc agctccacag gcactggcag ctctcctgcc gcactgccga
    42121 aatcaccaat tgccgtcgct ccaacgccta aacagaacgg tggcatcacg tcatagaaaa
    42181 agccgttgta gtgatctggc gcaaacagca ccaccagttc aggggagaaa gccgcaatac
    42241 gctcgcgggc gctggcaatc acgccattga cctcatcgag cacctcttgc gccgggtcga
    42301 cataccccac cagcggcgag tgggaaagac agtgaagata agcgtgcata tcaggctacc
    42361 ttttcgacag aaacgtcggc atcagggcgg gtcagcgtca tcaccgacgc cagtttattc
    42421 agggtcttgc ccagggtttg cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc
    42481 accaccagcg aagcattgtg ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc
    42541 acgcgtagca cgccgtcgtg gttatcctgt gcggtatgaa tttgcacttc cggcaccacc
    42601 tgaatgaagc gtgtgcccaa cgcgcgccac tgctggattt gctcgtcgct catcccccac
    42661 agtggattgc atccccagcc aattaccgcg aagttcgcgc caatcgcgtt atcgagcagc
    42721 gtcacgtcac cgttttccag cgtgactttc ggctgaataa acatcttgcc gaccggagag
    42781 tgcttcgctt cgccctcacg caccagcgca ccgccgtaat attgcggcat cggcttgaag
    42841 cgcatttcga ggaaatagcg ttttactggc ggcagataat tcaacagcca ggaaacgccg
    42901 tcacgtaacg taccctgcca gcgtttcggc ggagccagca cgttgcccgc cgtcacggac
    42961 aggtcaatca tcgctttggc gtgatcgcga cgttcttgtt gataggtatc gagcagcgca
    43021 tcgcgggctt tcccctggat aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc
    43081 ataccactgt tatagccctg cccctgccac accggcatga tgtgcgcggc atcgcccgcc
    43141 agcagtacgc gatcaatacg gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc
    43201 tggcgaatca attcaacatt gtccggatta ggcagcactt tgcttaacag cttgcgcata
    43261 ttttgcggct cacgcagctg ctcttcggtt tctcccggca tcaccataaa ttcaaagcga
    43321 cgtaccgcat gaggcagcgc ggcagaaaca tacgggcgca ccggatcgca acacaaatag
    43381 atatgcggcg tacttaacgg atcgttggcg atatctacca caatccactg atttggcgca
    43441 gttttacctt caaacggcac attcagagtg cgacggacaa aacttgctcc accgtcacag
    43501 gctaccagcc actgggcttt gaccgtttcc cgctgccctt ctgccgtttt caggtgcaag
    43561 gtcacttcgt cattttgctg gctgaaggcc tccagctcgc gggaaaacaa gcagcgcaca
    43621 ttcggaaaac gcgacaaccc ttccagcatc accgcatcga cctgaggctg aataaaggcg
    43681 ttacggcgcg gccagccaaa ttcatcggtc attggctgaa tatcagcaaa acagcggcct
    43741 ttcggggtga gaaaacgcat cgcgtgccac ggcgtagtgt gcggcagaac atcatcgacc
    43801 aggccgaccg actgcatggt gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag
    43861 tcgatcaact tatcgagttt ttccaccacc agcacgtcaa tgcccatctg gccgagatag
    43921 ttcgccatca tcagcccgac cggaccggca ccagcgatcg ccacctgaac gctatggtta
    43981 acagcaggct ggatgtcagg gtgttgtatt gccatttcag tacctcacga ctcggacaaa
    44041 atgccgttgc gcgcacagta cggcgcaact tattttgtta aaaacatgta aatgattttt
    44101 tattgtgcgc tcagtatagg aagggtgttt tcggctacaa tcaaaacatg cccgaatgtg
    44161 caccaggtgc acaactttgt tttaactata gaaatgtcaa ttaatatgca gaacaatgag
    44221 cagacggaat acaaaaccgt gcgcggctta acccgcggtc taatgttatt aaatatgtta
    44281 aataaacttg atggcggtgc cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc
    44341 accactgtgc ggcgactgct ggagacgctg caggaagagg gatatgtccg ccgtagcccc
    44401 tccgatgata gttttcgtct gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac
    44461 gaacagtgga tttctgcact ggcggcccca ctgctgggcg atctgttgcg cgaagtggta
    44521 tggccgacag atgtgtccac gctggatgtt gatgcaatgg tggtacgcga aaccactcac
    44581 cgtttcagcc gcttatcctt tcaccgggca atggtcgggc gacgtttgcc gcttctgaaa
    44641 accgcctctg gcctgacctg gctggccttt tgcccggaac aagaccgcaa ggaattaatc
    44701 gaaatgttag cctcccgccc cggtgatgac tatcaactgg cacgggaacc gttaaagctg
    44761 gaagccattc tggcgcgcgc gcgcaaagag ggttacggac agaactaccg cggctgggat
    44821 caggaggaga agatcgcctc tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc
    44881 tgtctgaatc tggtgtatat ggcgagcgca atgaccattg aacaggcagc ggaaaagcat
    44941 cttccggcgc tacaacgggt agcaaaacag atcgaagaag gggttgaatc gcaggctatt
    45001 ctggtggccg gacggcgaag cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac
    45061 ctttcgcggt atggcatgat agcgtccgga agagagtcaa ttcagggtgg tgaatgtgaa
    45121 accagtaacg ctatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
    45181 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
    45241 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
    45301 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
    45361 gattaaatct cgcgctgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
    45421 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
    45481 gatcattaac tatccgctgg atgaccagga tgccactgct gtggaagcag cctgcgctaa
    45541 tgttccggcg ttatttcttg atgtctctga ccagactccc atcaacagta ttattttctc
    45601 ccatgaagac ggtacgcggc tgggcgtgga gcatctggtc gcattgggtc accagcaaat
    45661 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg cgggctggca
    45721 taaatatctc acacgcaatc aaattcagcc gatagcggaa cgggagggcg actggagtgc
    45781 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
    45841 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
    45901 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcgtgtta
    45961 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
    46021 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
    46081 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
    46141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    46201 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    46261 ttccggctcg tatgttgtgt gaaattgtga gcggataaca attttacaca ggatacagct
    46321 atgactatga ttacggattc actggtcgtc gtattacaac gtcgtgactg ggaaaaccct
    46381 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    46441 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    46501 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
    46561 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
    46621 tacaccaacg tgacctatcc cattacggtc aatccgcctt ttgttcccgc ggagaatccg
    46681 acgggttgtt actcgctcac atttaatatt gatgaaagct ggctacagga aggccagacg
    46741 cgaattattt ttgatggtgt taactcggcg tttcatctgt ggtgcaacgg gcgcagggtc
    46801 ggttacggcc aggacagccg tttgccgtct gaatttgacc tgagcacatt tttacgcgcc
    46861 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
    46921 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca caaaccgagc
    46981 acacaaatca gcgatttcca tgttgccact cactttaatg atgatttcag ccgcgcggta
    47041 ctggaagcag aagttcagat gtatggcgag ctgcgcgatg agctgcgggt gacggtttct
    47101 ttgtggcagg gtgaaacgca ggtcgtcagc ggcaccgcgc ctttcggcgg tgaaattatc
    47161 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
    47221 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
    47281 ggcacgctga ttgaagcaga agccagcgat gtcggtttcc gcgaggtgcg gattgaaaat
    47341 ggtctgctgc tgctgaacgg caagccgttg ctgattcgcg gcgttaaccg tcacgagcat
    47401 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
    47461 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
    47521 acgctgtgcg accgctacgg cctgtatgtg gtagatgaag ccaacattga aacccacggc
    47581 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
    47641 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctggga
    47701 aatgagtcag gccacggcgc taatcacgac gcactctatc gctggaacaa atctgtcgat
    47761 ccatcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
    47821 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
    47881 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
    47941 cacgcgatgg gtaacagtct tggcggcttc gctaaatact ggcaggcgtt tcgtcagtat
    48001 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
    48061 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
    48121 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
    48181 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
    48241 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
    48301 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
    48361 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
    48421 gtagtgcaac cgaacgcgac cgcatggtca gaagccggac acatcagcgc ctggcagcag
    48481 tggcgtctgg cggcaaacct cagcgtgaca ctcccctccg cgccccacgc catcccgcaa
    48541 ctgaccacca gcgaaacgga tttttgcatc gagctggata ataagcgttg gcaatttaac
    48601 cgccagtcag gctttctttc acagatgtgg attggcgatg aaaaacaact gctgaccccg
    48661 ctgcgcgatc agttcatccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
    48721 cgtattgacc cgaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
    48781 gcggcgttgt tgcagtgcac ggcagataca cttgccgacg cggtgctgat taccactgtc
    48841 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
    48901 ggtagtggtc aaatggcgat taccgttgat gttgaagtag cgagcgatac accgcatccg
    48961 gcgcggattg gcctgacctg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
    49021 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
    49081 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
    49141 gggacgcgcg aattgaatta tggctcacac cagtggcgcg gcgacttcca gttcaatatc
    49201 agtcgctaca gccaacaaca actgatggaa accagccatc gccatctgct gcacgcggaa
    49261 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
    49321 agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccaattggtc
    49381 tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa
    49441 tccattatgt actatttaaa aaacacaaac ttttggatgt tcggttttta ttctttttct
    49501 tttacttttt tatcatggga gcctacttcc cgtttttccc gatttggcta catgacatca
    49561 accatatcag caaaagtgat acgggtatta tttttgccgc tatttctctg ttctcgctat
    49621 tattccaacc gctgtttggt ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt
    49681 ggattattac cggcatgtta gtgatgtttg cgccgttctt tatttttatc ttcgggccac
    49741 tgttacaata caacatttta gtaggatcga ttgttggtgg tatttatcta ggcttttgtt
    49801 ttaacgccgg tgcgccagca gtagaggcat ttattgagaa agtcagccgt cgcagtaatt
    49861 tcgaatttgg tcgcgcgcgg atgtttggct gtgttggctg ggcgctgtgt gcctcgattg
    49921 tcggcatcat gttcaccatc aataatcagt ttgttttctg gctgggttct ggctgtgcac
    49981 tcatcctcgc cgttttactc tttttcgcca aaacggatgc gccctcttcc gccacggttg
    50041 ccaatgcggt aggtgccaac cattcggcat ttagccttaa gctggcgctg gaactgttca
    50101 gacagccaaa actgtggttt ttgtcactgt atgttattgg cgtttcctgc acctacgatg
    50161 tttttgacca acagtttgct aatttcttta cttcgttctt tgctaccggt gaacagggta
    50221 cgcgggtatt tggctacgta acgacaatgg gcgaattact taacgcctcg attatgttct
    50281 ttgcgccact gatcattaat cgcatcggtg ggaaaaacgc cctgctgctg gctggcacta
    50341 ttatgtctgt acgtattatt ggctcatcgt tcgccacctc agcgctggaa gtggttattc
    50401 tgaaaacgct gcatatgttt gaagtaccgt tcctgctggt gggctgcttt aaatatatta
    50461 ccagccagtt tgaagtgcgt ttttcagcga cgatttatct ggtctgtttc tgcttcttta
    50521 agcaactggc gatgattttt atgtctgtac tggcgggtaa tatgtatgaa agcatcggtt
    50581 tccagggcgc ttatctggtg ctgggtctgg tggcgctggg cttcacctta atttccgtgt
    50641 tcacgcttag cggccccggc ccgctttctc tactgcgtcg tcaggtgaat gaagtcgctt
    50701 aagcaatcaa tgtcggatgc ggcgcgagcg ccttatccga ccaacatatc aggacggaat
    50761 gatcgcaatg aacatgtcga tgacagaaag aataaaagca ggcaagctat ttaccgatat
    50821 gtgcgaaggc ttaccggaaa aaagacttcg tgggaaaacg ttaatgtatg agtttaatca
    50881 ctcgcatcca tcagaagttg aaaaaagaga aagcctgatt aaagaaatgt ttgccacggt
    50941 aggggaaaac gcctggatag aaccacctgt ctatttctct tacggttcca acatccatat
    51001 aggccgcaat ttttatgcaa atttcaattt aaccattgtc gacgactaca cggtaacgat
    51061 cggtgataac gtattgattg cacccaatgt tactctttcc gttacgggac accctgtaca
    51121 ccatgaattg agaaaaaacg gtgagatgta ctcttttccg ataacgattg gcaataacgt
    51181 ctggatcgga agtcatgtgg ttattaatcc aggcgtcacc atcggggata attctgttat
    51241 tggcgcgggt agtgtcgtca taaaagacat tccaccaaac gtcgtggctg ctggcgttcc
    51301 ttgccgggtt attcgcgaaa taaacgaccg ggataagcaa tattatttca gagattacaa
    51361 agttgaatcg tcagtttaaa ttataaaaat tgcctgatac gct
//
LOCUS       DAHBHC010000028        50021 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHBHC010000028 DAHBHC010000000
VERSION     DAHBHC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.028"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..146
                     /locus_tag="M2A35_002039"
     CDS             <1..146
                     /locus_tag="M2A35_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCB7198345.1"
                     /translation="LGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQ
                     ADF"
     gene            205..408
                     /locus_tag="M2A35_002040"
     CDS             205..408
                     /locus_tag="M2A35_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCB7198346.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            408..1433
                     /locus_tag="M2A35_002041"
     CDS             408..1433
                     /locus_tag="M2A35_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7198347.1"
                     /translation="MGRRSKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASACYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLCVAPLPVFIAMEIAYLCAARVSDVLSLKWEQVGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1486..1575)
                     /locus_tag="M2A35_002042"
     tRNA            complement(1486..1575)
                     /locus_tag="M2A35_002042"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1539..1541),aa:Ser,seq:cga)
     gene            1669..2466
                     /gene="mtfA"
                     /locus_tag="M2A35_002043"
     CDS             1669..2466
                     /gene="mtfA"
                     /locus_tag="M2A35_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCB7198348.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2567..2642
                     /locus_tag="M2A35_002044"
     tRNA            2567..2642
                     /locus_tag="M2A35_002044"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2600..2602,aa:Asn,seq:gtt)
     gene            2956..10033
                     /gene="yeeJ"
                     /locus_tag="M2A35_002045"
                     /pseudo
     CDS             2956..10033
                     /gene="yeeJ"
                     /locus_tag="M2A35_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(10295..11347)
                     /locus_tag="M2A35_002046"
     CDS             complement(10295..11347)
                     /locus_tag="M2A35_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCB7198349.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11662..12978
                     /gene="shiA"
                     /locus_tag="M2A35_002047"
     CDS             11662..12978
                     /gene="shiA"
                     /locus_tag="M2A35_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCB7198350.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13080..14534
                     /gene="amn"
                     /locus_tag="M2A35_002048"
     CDS             13080..14534
                     /gene="amn"
                     /locus_tag="M2A35_002048"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCB7198351.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            14877..15593
                     /locus_tag="M2A35_002049"
     CDS             14877..15593
                     /locus_tag="M2A35_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCB7198352.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(16046..16121)
                     /locus_tag="M2A35_002050"
     tRNA            complement(16046..16121)
                     /locus_tag="M2A35_002050"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16086..16088),aa:Asn,seq:gtt)
     gene            complement(16222..17865)
                     /gene="yeeO"
                     /locus_tag="M2A35_002051"
     CDS             complement(16222..17865)
                     /gene="yeeO"
                     /locus_tag="M2A35_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCB7198353.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSVLRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTCGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            17870..17945
                     /locus_tag="M2A35_002052"
     tRNA            17870..17945
                     /locus_tag="M2A35_002052"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17903..17905,aa:Asn,seq:gtt)
     gene            complement(17983..18933)
                     /gene="cbl"
                     /locus_tag="M2A35_002053"
     CDS             complement(17983..18933)
                     /gene="cbl"
                     /locus_tag="M2A35_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCB7198354.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(19035..19952)
                     /gene="nac"
                     /locus_tag="M2A35_002054"
     CDS             complement(19035..19952)
                     /gene="nac"
                     /locus_tag="M2A35_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCB7198355.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVVVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            20279..20354
                     /locus_tag="M2A35_002055"
     tRNA            20279..20354
                     /locus_tag="M2A35_002055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20312..20314,aa:Asn,seq:gtt)
     gene            complement(20410..21345)
                     /gene="ldtA"
                     /locus_tag="M2A35_002056"
     CDS             complement(20410..21345)
                     /gene="ldtA"
                     /locus_tag="M2A35_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCB7198356.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(21407..22486)
                     /gene="cobT"
                     /locus_tag="M2A35_002057"
     CDS             complement(21407..22486)
                     /gene="cobT"
                     /locus_tag="M2A35_002057"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCB7198357.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(22498..23241)
                     /gene="cobS"
                     /locus_tag="M2A35_002058"
     CDS             complement(22498..23241)
                     /gene="cobS"
                     /locus_tag="M2A35_002058"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCB7198358.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTYGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(23238..23783)
                     /gene="cobU"
                     /locus_tag="M2A35_002059"
     CDS             complement(23238..23783)
                     /gene="cobU"
                     /locus_tag="M2A35_002059"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCB7198359.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMSARI
                     EHHRQGRPAHWRTVERWQHVDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            23974..24141
                     /locus_tag="M2A35_002060"
     CDS             23974..24141
                     /locus_tag="M2A35_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCB7198360.1"
                     /translation="MGVVTKADLANMEQISLVKSWLREAGAHNVLVTSAVNNNGVTEL
                     FALLHTEEGCC"
     gene            complement(24242..24571)
                     /locus_tag="M2A35_002061"
     CDS             complement(24242..24571)
                     /locus_tag="M2A35_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCB7198361.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(24743..25801)
                     /locus_tag="M2A35_002062"
     CDS             complement(24743..25801)
                     /locus_tag="M2A35_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCB7198362.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(26000..26473)
                     /gene="sbmC"
                     /locus_tag="M2A35_002063"
     CDS             complement(26000..26473)
                     /gene="sbmC"
                     /locus_tag="M2A35_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCB7198363.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSQNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(26592..27758)
                     /gene="dacD"
                     /locus_tag="M2A35_002064"
     CDS             complement(26592..27758)
                     /gene="dacD"
                     /locus_tag="M2A35_002064"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCB7198364.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIQAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            27967..29394
                     /gene="sbcB"
                     /locus_tag="M2A35_002065"
     CDS             27967..29394
                     /gene="sbcB"
                     /locus_tag="M2A35_002065"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCB7198365.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGIHWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(29437..29664)
                     /locus_tag="M2A35_002066"
     CDS             complement(29437..29664)
                     /locus_tag="M2A35_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCB7198366.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(29678..30736)
                     /gene="yeeE"
                     /locus_tag="M2A35_002067"
     CDS             complement(29678..30736)
                     /gene="yeeE"
                     /locus_tag="M2A35_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCB7198367.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(30915..32273)
                     /gene="plaP"
                     /locus_tag="M2A35_002068"
     CDS             complement(30915..32273)
                     /gene="plaP"
                     /locus_tag="M2A35_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCB7198368.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(32263..32325)
                     /gene="yoeI"
                     /locus_tag="M2A35_002069"
     CDS             complement(32263..32325)
                     /gene="yoeI"
                     /locus_tag="M2A35_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCB7198369.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(32540..33469)
                     /locus_tag="M2A35_002070"
     CDS             complement(32540..33469)
                     /locus_tag="M2A35_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7198370.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTAPQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(33515..34339)
                     /locus_tag="M2A35_002071"
     CDS             complement(33515..34339)
                     /locus_tag="M2A35_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7198371.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTSRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(34422..34676)
                     /gene="yoeB"
                     /locus_tag="M2A35_002072"
     CDS             complement(34422..34676)
                     /gene="yoeB"
                     /locus_tag="M2A35_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCB7198372.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(34673..34924)
                     /gene="yefM"
                     /locus_tag="M2A35_002073"
     CDS             complement(34673..34924)
                     /gene="yefM"
                     /locus_tag="M2A35_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCB7198373.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            35208..35258
                     /gene="hisL"
                     /locus_tag="M2A35_002074"
     CDS             35208..35258
                     /gene="hisL"
                     /locus_tag="M2A35_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCB7198374.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    35234..35357
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            35404..36303
                     /gene="hisG"
                     /locus_tag="M2A35_002075"
     CDS             35404..36303
                     /gene="hisG"
                     /locus_tag="M2A35_002075"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCB7198375.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            36309..37613
                     /gene="hisD"
                     /locus_tag="M2A35_002076"
     CDS             36309..37613
                     /gene="hisD"
                     /locus_tag="M2A35_002076"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCB7198376.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVARQLAVLPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            37610..38680
                     /gene="hisC"
                     /locus_tag="M2A35_002077"
     CDS             37610..38680
                     /gene="hisC"
                     /locus_tag="M2A35_002077"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCB7198377.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            38680..39747
                     /gene="hisB"
                     /locus_tag="M2A35_002078"
     CDS             38680..39747
                     /gene="hisB"
                     /locus_tag="M2A35_002078"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCB7198378.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            39747..40337
                     /gene="hisH"
                     /locus_tag="M2A35_002079"
     CDS             39747..40337
                     /gene="hisH"
                     /locus_tag="M2A35_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCB7198379.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQEAMDQVRERDLFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            40337..41074
                     /gene="hisA"
                     /locus_tag="M2A35_002080"
     CDS             40337..41074
                     /gene="hisA"
                     /locus_tag="M2A35_002080"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCB7198380.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRSVIVGRALLEGKFTVKEAIACWQNA"
     gene            41056..41832
                     /gene="hisF"
                     /locus_tag="M2A35_002081"
     CDS             41056..41832
                     /gene="hisF"
                     /locus_tag="M2A35_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCB7198381.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     EWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            41826..42437
                     /locus_tag="M2A35_002082"
     CDS             41826..42437
                     /locus_tag="M2A35_002082"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCB7198382.1"
                     /translation="MLTEQQCRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
     gene            complement(42609..43586)
                     /gene="wzzB"
                     /locus_tag="M2A35_002083"
     CDS             complement(42609..43586)
                     /gene="wzzB"
                     /locus_tag="M2A35_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCB7198383.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPMLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(43733..44899)
                     /gene="ugd"
                     /locus_tag="M2A35_002084"
     CDS             complement(43733..44899)
                     /gene="ugd"
                     /locus_tag="M2A35_002084"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCB7198384.1"
                     /translation="MNITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDTNEAYQDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDTFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(45148..46554)
                     /gene="gndA"
                     /locus_tag="M2A35_002085"
     CDS             complement(45148..46554)
                     /gene="gndA"
                     /locus_tag="M2A35_002085"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCB7198385.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAST
                     FTEWNNGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAHPAGDKVEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYHWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAKNAGIANLLLDPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
     gene            complement(46735..47601)
                     /gene="rfbA"
                     /locus_tag="M2A35_002086"
     CDS             complement(46735..47601)
                     /gene="rfbA"
                     /locus_tag="M2A35_002086"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014700618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCB7198386.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPIYDKPMVYYPISVLMLA
                     GIKDILLISTPEDLSSYKRLLGDGSRFGINLQYAIQPSPDGLAQAFIIGEEFIAGDEC
                     ALVLGDNIFFGQSFGRKLEQVASRKRGATVFGYQVMDPERFGIVEFDESFKVLSVEEK
                     PQTPKSNWAITGLYFYDNQVVDFAKQVKPSPRGELEITSINKMYLERDMLNVELLGRG
                     FAWLDTGTHDSLIEASQFVHTIEKRQGYKVACLEEIALRNKWLTAEQVLTEARIMEKT
                     QYGQYLFQLIGK"
     gene            complement(47746..48276)
                     /locus_tag="M2A35_002087"
     CDS             complement(47746..48276)
                     /locus_tag="M2A35_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCB7198387.1"
                     /translation="MKNIQQDIQRYKELIGNESAFKFKDYFRIFSPRLIPNVIYRTSY
                     SLKEKGFRRISKCCSLLNVLIFNIEIATECEIKGGLFIPHTFGIVIGAARIGENCTIY
                     QNVTIGAKTLDMKYNKELRPVIGNDVILATGSVILGPIVINNGAIVAANSVVLKDVGE
                     KELVGGTPATYLKKLD"
     gene            complement(48242..49333)
                     /locus_tag="M2A35_002088"
     CDS             complement(48242..49333)
                     /locus_tag="M2A35_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HCB7198388.1"
                     /translation="MANIYFLFGTLFIFSPVFFIIQFCVFFLVFIQGKCKVSSQGLIF
                     ILVSFTSLIFHVFFGVVDKIFIFTLVCCFYALTVPSIYSYCVKRIKSCDWLKVIDFCI
                     KFHIFTFLLQYLAFKIINVDIDYGKFLLGPPHRSSYDGFDYRATGVFAEPSIFSIHMV
                     TLLVMRYIVANNNSLLSYIALMCMVLSGSTFSVICVMLFFILTCRLTLKWIATGLISL
                     VLVSPIIYQNIMWRMSYISSGSDGSTSYKLELMKSFFDSPLVFNIGHGMVGYYDNAPD
                     YFQSLFDMTLFGSNFIVFGFWLGSILSVSWLVLFLKYSRFSLRLMLLCLLPCLKLTFL
                     FPMFWLYLVFLAGYAGIKNEKYTTRYSKI"
     gene            complement(49326..>50021)
                     /locus_tag="M2A35_002089"
     CDS             complement(49326..>50021)
                     /locus_tag="M2A35_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCB7198389.1"
                     /translation="WLFFVDIDNLLQQGCLDSLHSMILSCEKPFVARANVRCRQDLTR
                     DSNYLHYFDSRYLGNRYKGTVKIEYRYFASDAFLISKSEFEAQKGFDESFRHYGCEDE
                     EFGARLRLSSVPFFFCSDALLYDNDTPTLDRACERMIPYARYSVPLLVKKHPEIVDKL
                     LFPQAERKGSMKSTVLRVALSCANSIKLNKFVRVVLNKLDNRNKFKIRAIFYKLVLAM
                     YYIEGYSKRERNG"
BASE COUNT        13664 a        12430 c        11550 g        12377 t
ORIGIN      
        1 agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa ggtgagcgcc
       61 ataatgcact tgatgacgcc cgttaccagg caaaatacgt ttcagctatc tggcaaaaac
      121 tgatcccgag tcaggctgat ttttaatgtt caaccctgat cgccgctaac cgcatatagt
      181 tagcggcggt tatgagatat agctatgagc agcttatttt taaccgaaga tgaattgcta
      241 atattaacgg gctgcaaata tgcaagccac cagcgaaaat ggttaatgga aaacgggctt
      301 ccgttctata ccaatcgtag tggcaaaccg attgtcagcc gggatctatt tacctgcaat
      361 aaaactttac caccacgcga ggtagagccg aattttggtg cgatctgatg ggaagacgaa
      421 gcaaaaatcc tgaacacgaa aaattacctc caaatgtata tccaaataaa tatagttatg
      481 tatggaaacc aacatccaga gaatctgtaa cactaaccgc catcaaggat ggtttagctg
      541 ctttatggaa aaagtatgag gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc
      601 gcttgtggga aaaattcctc gccagcgcct gttacagtga ccttagtcca agaacacaaa
      661 aagattatct gcaacatcaa aaaaagttgc ttgccgtatt cggtaaggta ccagcggatt
      721 ccataaaacc agaacacatc cgtcgataca tggacaaaag aggggaacag agtaaaacgc
      781 aagccaacca tgaaaaaagc agtatgtccc gcgtttacag ttggggatat gagcgagggt
      841 acgtgaaggc taacccatgt gcaggtgtaa gtaaattcaa ggccaaaaac cgcgaacgat
      901 atgtaaccga caaagaatac caggcagtat tatgcgttgc accccttcct gtttttatcg
      961 caatggaaat tgcctatctg tgtgcagcga gggtttccga tgtgttatcg ctgaaatggg
     1021 aacaggttgg aaacgacggg atctttatcc agcaagggaa aacagggaaa aaacagataa
     1081 aagcatggag tccacgatta caggcggcga tcgaaaaagc aaaacagtta ccaacatccg
     1141 cctatgtaat cagcaatcaa tatggcaacc gatatatgta caaaggcttt aacgaaatgt
     1201 gggtagaagc aagaaatcgc gcaggcaaaa tttcaggtat tttaaccgac ttcacctttc
     1261 atgatctgaa ggcgaaagga atttcagact atgaaggaag cagccgggat aagcaacttt
     1321 tctctggtca caaaaccgaa gggcaggtgc taatctatga cagaaaggtt aaagtttcac
     1381 caacacttga tgtcccgtta cctgaaaata ttccaagaaa atattccaag taattccaag
     1441 tgtgattttt gtcactgact taatgatgtg taagtgattg aattttggcg gagagagggg
     1501 gatttgaacc cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg
     1561 ctccggcatc tctccgttca gatggttgcc atgatgccag gaaatttggc attttaacag
     1621 tccctgtccg tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg
     1681 ccctggaaag tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg
     1741 atcccccttt taacgtgtct gacggaacag gaacaaagca aattagtcac tcttgccgaa
     1801 cgttttttac agcaaaagcg gcttgttcct ttacagggct ttgaactgga ttcattaaga
     1861 agctgccgga tagcacttct attttgccta cccgttctgg agttaggact ggaatggctg
     1921 gatggttttc atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa
     1981 gacgatatcg gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa
     2041 gggcctatcg ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac
     2101 ctgattattc atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga
     2161 gttcccttta ttccgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg
     2221 aacaacattc aggaagaaat cgaattggtt ggcgagaatg cggcgagcat tgatgcttat
     2281 gctgccagtg atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca
     2341 gaactttttg ctcctcgttt cccttcattg tggcaacgtt tctgccaatt ttatcaacaa
     2401 gatcctttgc agagactgca tcgcactaat gatacagact cgttttcggc gacgaatgtt
     2461 cattaattaa caactttgca gattaattaa ccaattgaaa tgacttatga aatttagtgt
     2521 tgacagacaa ggtaccgcta agtaatatgc gccccgttca cacgattcct ctgtagttca
     2581 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     2641 caaattctaa aaattcgctt ttttagcgca atgtcactga ccttagttga acattgtttt
     2701 ttaacggata gcgggttttt aacatcttaa gcgccctcga cctttatggt tgagggcgtt
     2761 ttgctatgaa cgccatcacc attttcccct cgattataaa acttgagtta ttcagtagtc
     2821 tcccctcttg caactcacac ccaaaactgc ctaacgaaaa gttattaatt ttcaatcata
     2881 ttgctatcag gatttacatt ttttcgctgt gctagaaagg gcgcatttat gttagctcgt
     2941 tcagggaagg taagcatggc tacgaagaag agaagtggag aagaaataaa tgaccgacaa
     3001 atcttatgcg ggatgggaat taaactacgc cgcttaactg cgggtatctg cctgataact
     3061 caacttgcgt tccctatggc tgcggcagca caaggtgtgg taaacgccgc aacccaacaa
     3121 ccagttcctg cacaactcgc cattgcaaat gccaatacgg tgccctacac ccttggagcg
     3181 ctggaaatcg gcccaaagcg ttgccgaacg tttcggtatt tcggtggctg agttacgcaa
     3241 actcaaccag tttcgtacgt ttgctcgagg ttttgataat gtccgccagg gtgatgaact
     3301 ggatgtcccg gcacaagtta gtgaaaataa tttaaccccg ccaccgggta atagcagcgg
     3361 caaccttgag caacagatag ccagtacttc acagcaaatc gggtctctgc tcgccgagga
     3421 tatgaacagc gagcaagcgg caaatatggc gcgtggatgg gcctcttctc aggcttcagg
     3481 cgcaatgaca gactggttaa gccgcttcgg taccgcaaga atcacgctgg gcgtggatga
     3541 agattttagc ctgaagaact cccagttcga ttttctccat ccgtggtatg aaacgcctga
     3601 taatctcttt ttcagtcagc atactctcca tcgtactgac gagcgtacac agattaacaa
     3661 cggcttaggt tggcgtcatt tcactcccac atggatgtcg ggcatcaact tctttttcga
     3721 ccacgatctt agccgttacc actcccgcgc cggcattggc gcggagtact ggcgcgacta
     3781 tctaaaatta agcagtaacg gctatttgcg actgaccaac tggcgcagcg cacctgaact
     3841 ggacaacgat tatgaagcac gcccggccaa tggctgggat gtacgcgcag aaggctggct
     3901 acccgcctgg ccgtaccttg gcggtaaact ggtctatgaa cagtattatg gcgatgaagt
     3961 ggccctgttc gataaagacg atcggcaaag taatcctcat gccataaccg ctggacttaa
     4021 ctataccccc ttcccgctaa tgaccttcag cgcggagcaa cgccagggta aacagggcga
     4081 aaatgacacc cgttttgccg tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca
     4141 gcttgacccg aatgaagtcg ctgcacggcg tagccttgca ggcagccgtt atgatctggt
     4201 ggatcgcaac aacaacatcg ttctggaata tcgcaaaaaa gaactggtcc gcctgaccct
     4261 gacagacccc gtgacaggga agtcaggaga agtgaaatca ctggtttcgt cgctacaaac
     4321 caaatatgcc ctgaaaggct ataacgtcga agccaccgca ctggaagctg ccggtggcaa
     4381 agtggtcaca acgggtaaag atattctggt taccctgccg ggttaccggt tcaccagtac
     4441 gccagaaacc gataacacct ggccgattga agtcaccgct gaagatgtca aaggcaattt
     4501 gtcgaatcgt gaacaaagca tggtggtcgt tcaggcacct acgctaagcc agaaagattc
     4561 ctcggtatcg ttaagtaccc aaacattgaa cgcggattcc cattcaaccg ccacactgac
     4621 ttttattgcg catgatgcag caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca
     4681 cgaaggtgtt caggacatca ccctttctga atggaaagat aatggtgacg gaagctatac
     4741 ccagatcctg accacaggag cgatgtctgg cacgctgacg ctgatgccac agctgaatgg
     4801 tgtggatgcg gctaaagccc ccgccgtggt gaatatcatt tctatttcgt catcccgaac
     4861 tcactcgtca attaaaattg ataaagaccg ttatctctcc ggcaatccta tcgaggtgac
     4921 ggtagaactg agagatgaaa atgacaaacc tgttaaggag caaaaacagc aactgaataa
     4981 cgcagtcagc atcgacaacg tgaaacctgg tgtcactaca gactggaaag aaaccgcaga
     5041 tggcgtctat aaggcaacct ataccgccta taccagaggc agtgggctta ctgcgaagct
     5101 gttaatgcaa aactggaatg aagatttgca taccgctgga tttatcatcg acgccaaccc
     5161 gcagtcagca aaaattgcga cattatctgc cagcaataat ggtgtgctcg ccaatgagaa
     5221 tgcagcaaac accgtctcgg tcaatgtcgc tgatgaagga agcaacccaa tcaatgatca
     5281 taccgtcacg tttgcggtat taagcgggtc ggcaacttgc tttaacaatc aaaacaccgc
     5341 aaaaacggat gttaatggtc tggcgacttt tgatctgaaa agtagtaagc aggaagacaa
     5401 cacggttgaa gtcacccttg aaaatggcgt gaaacaaacg ttaattgtca gttttgtcgg
     5461 cgactcgagt accgcgcagg ttgatctgca gaagtcgaaa aatgaagtgg tcgctgacgg
     5521 caatgatagc gccacaatga ccgcgacagt ccgggatgca aaaggcaacc tgctcaatga
     5581 cgtcaaggtc actttcaatg ttaattcagc agcagcgaaa ctgagccaaa ccgaagtgaa
     5641 tagccacgac gggatcgcca cagctacgct gaccagtttg aaaaatggtg attatagggt
     5701 tacggcctct gtgagctctg gttctcaggc taatcaacag gtgattttta tcggtgatca
     5761 aagtactgct gccctgaccc tcagtgtgcc ttcaggtgat atcaccgtca ccaacacagc
     5821 tccgctacat atgactgcaa ccttgcagga taaaaatggc aacccattaa aagataaaga
     5881 aatcaccttc tctgtgccaa acgacgtcgc aagtcggttc tcgattagca acagcggaaa
     5941 aggcatgacg gatagcaacg ggactgcaat cgcctccctg accggcacgt tagcgggcac
     6001 gcatatgatc acggctcgtc tggctaacag caatgtcagc gatacacagc caatgacgtt
     6061 tgtggcggat aaagacagag cggttgtcgt tctgcaaaca tcgaaagcgg aaatcattgg
     6121 gaatggcgtg gatgagacaa ctctgacagc aacagtgaaa gatccgtcga atcatccggt
     6181 ggcggggata acggtcaact tcaccatgcc acaggacgtt gcggcaaact ttacccttga
     6241 aaataacggt attgccatca ctcaggccaa tggggaagcg catgtcacgc tgaaaggtaa
     6301 aaaagcgggc acgcatacgg ttaccgcaac gctgggtaat aacaatacca gtgattcgca
     6361 gccggtaaca tttgtggcgg acaaagcctc ggctcaggtt gtcctgcaga tatcaaaaga
     6421 tgagatcaca ggtaatggcg tcgatagcgc aacgctaact gcaacggtta aagatcagtt
     6481 cgacaatgag gtgaataatc ttccggtaac attcagctca gcctcttcag gactcaccct
     6541 gaccccggga gtgagtaata ccaatgagtc tggcatcgcg caggccacta tcgcaggcgt
     6601 tgcctttggt gagcagacgg tcacggcatc actggctaat aatggtgcca gcgacaacaa
     6661 aactgtgcat tttattggcg acacagcggc ggcaaaaatt atcgagttga cgcctgtccc
     6721 agacagcata atcgccggta ccccgcagaa cagcaccggc agcgtcatca ccgccacagt
     6781 cgttgataat aatggctttc cggtgaaagg tgtgactgtg aacttcacca gccgaacaaa
     6841 ctctgccgaa atgacgaatg gcggccaagc cgtaacgaac gaacagggta aggcaaccgt
     6901 cacttatacc aatacccgct cctcgataga atcaggagcg agaccggata ccgttgaggc
     6961 cagtctggaa aatggtaact ccacgcttag cacatcaatt aatgtcaacg cggatgcgtc
     7021 tacagcacat ctcaccttgc tacatgcact ttttgataca gtctccgcag gcgagacaac
     7081 cagtctgtat attgaggtga aggataatta cggcaatggt gtaccccagc atcaggtaac
     7141 cctcagcgtt tcaccaagtg aaggcgtgac cctcagtaat aacggtatat atactaccaa
     7201 ctactacggc tatttttacg caagctttac cgctacaaaa gccggggttt atcaagtgac
     7261 ggcaaccctc gacaatggcg attcgatgca acaaacagtg acctatgtgc cgaacgtcgc
     7321 gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg attgccgaca ataacgatct
     7381 cacaacacta acagcaacag tcgctgatac agagggcaat gcgatagcca acactgaggt
     7441 aacatttact ctgccggaag atgtgagggc gaacttcacg ctgagcgatg gcggtaaagc
     7501 gattactgat actgaaggca aagcgaaagt cacgctgaaa ggtataaaag caggcgctca
     7561 tactgttaca gcatcgatgg ctggcagtaa gagtggacaa ctggtggtga actttactgc
     7621 ggatacgctc actgcgcagg ttaatcttaa cgttaccgag gacaatttca tcgccaataa
     7681 cattgggatg accaagctgc aggcaacagt gactgatgga aacggcaacc cattcgccaa
     7741 tgaggcggtg acattcacgc taccggcaga tgtgagcgca agcttcactc tcggacaagg
     7801 cggttccgcc attactgata tcaacggcaa ggctgaagtt acactgagcg gtaccaaatc
     7861 cggcacctat cccgtgacag ttagcgtgat caattatggt gtcagtgata cgaaacaggt
     7921 gactttgatt gccgatgctg gtaccgcaac actagcctcc ttaacctctg tatactcatt
     7981 cgtcgtcagc acgaccgagg gcgcgaccat gactgcaagc gtcactgacg ctaacggcaa
     8041 cccggtagaa ggcataaaag ttaatttccg cggaacctcc gtcacgctaa gcagcaccag
     8101 cgttgaaacg gatgatcagg gtttcgctga aattcttgtg acaagcaccg aagtcggact
     8161 gaaaacagtt tcagcctctc tggcagataa acctactgaa gtcatatcgc gattactgaa
     8221 tgcaaaagca gatattaatt ctgcaacgat taccagtctg gagatacctg aaggtcagct
     8281 aatggtcgca caagacgtag cagttaaagc tcacgtcaac gaccagtttg gcaacccgat
     8341 tcttaatgaa tctgtaacat tcagtgcaga gccaccagag cacatgacca tcagccaaaa
     8401 tattgtctct actgatacgc atggtatagc cgaggtctcc atgacgcccg aaagaaacgg
     8461 ttcgtatatg gtgaaagcat ccctggcgaa tggagcctca cttgagaaac aactggaggc
     8521 tattgatgaa aaactgacac tcacggcgtc cagtccgctt atcggtgtct atgcccctac
     8581 aggcactact ctgacggcaa cgctaacctc tgcaaatggc actccagtgg agggtcaggt
     8641 catcaacttt agcgtaacgc cagaaggggc gacgttaagt ggcggaaaag tgagaactaa
     8701 ctcttcaggt caggctccgg tcgttctgac cagcaataaa gtcggtacat atacggtgac
     8761 tgcatcgttc cataacggcg taacaataca gacacagaca accgtgaaag tcactggcaa
     8821 ctcaagcacc gcacatgttg ctagctttat cgctgatcca tcgactatcg ccgccaccaa
     8881 cagtgattta agtaccttaa aggcaacggt tgaggatggc agtggtaacc tgatcgaagg
     8941 tctcactgtg tacttcgcct taaaaagcgg ctctgccaca ttaacgtcat taacagcggt
     9001 gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga gcgatgacag gtagcgtcac
     9061 ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta gatataacgc tggtggcagg
     9121 cccggcagac gcctcgcagt ccgtccttaa gaacaatcgg tcctcactga aaggggactt
     9181 taccgatagt gctgagctac atcttgtttt gcacgatata tcaggcaatc cgatcaaagt
     9241 ttctgaaggg atggaatttg tgcaatcagg tactaacgtg ccctatatga aaattagcgc
     9301 aattgattac agtcaaaata tcaacggtga ttacaaagcc accattacag gcggcggaga
     9361 gggtatcgca acgctgatcc ctgtattgaa tggtgttcat caagctggtc tgagtaccac
     9421 aatacaattc actcgcgcag aagacaaaat aatgagcggt acagtatcag tcaatggtac
     9481 tgacctaccg acaactacat tcccttcgca ggggttcacc ggggcgtatt atcagttgaa
     9541 taatgacaac tttgccccag gaaaaacggc ggctgattat gagttttcaa gctctgcctc
     9601 ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa aatgtcggca gcaattggga
     9661 aaggattacg gcgacgccaa aatcaggagg ccctagctat gtatacgaaa tccgtgtgaa
     9721 gagttggtgg gtgaactccg gcgatgcttt catgatatac agccttgctg aaaatttttg
     9781 cagtagcaat ggctacacac ttccccgtgc agaccattta aaccatagtc gttcccgagg
     9841 catcgggtca ctgtacagtg aatggggaga tatggggcat tacacgactg aagctggttt
     9901 tcaatcaaat atgtattggt catctagtcc cgcaaactca agcgaacaat acgtagtttc
     9961 cctggcaaca ggtgatcaaa gcgtatttga aaagcttggg tttgcttatg cgacatgtta
    10021 taaaaacctg tgattttccg ttataatttc ttaaagagtg ctccattcgg agcactcttt
    10081 tttacattcc ctctatagag cttgcccttc tctcacagaa aatgaataaa taaaactgct
    10141 gcaccgcaag aatcgcgttc gatgtgacta tgatcgtatc cggtgagtac agctttgatc
    10201 caaaggctgg ttttctgtga tgattgacca gcctcagagt attgatgaca ccaccgttca
    10261 taacagatcg gcattactat gcataagtac tgtctcatat tctcgccaaa acacttttta
    10321 atgcctttgc gaattcatcc agacttatat tgtcaatttt ttgcccaacg acgactgact
    10381 ccgaaacatc ggccattgcg ggccatcgcg ttttgtaatc agcataaatt gcaacaacgg
    10441 gagtattata ggcacttgcc atatgtaata ttgaggtgtc aggagttatt gcgatataag
    10501 catcttttat gattgctgct gaacgtttaa tcgagggtga taaggataaa cggtagacat
    10561 tattataaca atccactaat accctggctt tatcttcccc ttttggtcca tgaacgatca
    10621 ctattggtat atctgtttca ctttgaattt ttgctattag attttctgca atcgataatg
    10681 aaaatgtacg ttcctgcgag ctaccttcta aattaagcgc aatgtaagat cctaacgatc
    10741 gcatttcctc gcgcacctca tcgagtacat cctcacttag tggcaattca tatattggcc
    10801 tgactgctgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt acaggagccc
    10861 ggagattctg atccatccgg gacgcgttct tgcacaaagg ggagtagcac ttcatggtta
    10921 aaccaacaac ctgaaaattc gtttttgctt tcaactgact gataaatatc atcgttttca
    10981 ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accgtattgc tgtgttatgt
    11041 tctttgctaa atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc attgtgacta
    11101 atgttaattt agtcttttca agtttaagtg cattaaacac ttctaaatta attgtcgacg
    11161 taacaattaa aagatgctta attttatgca attcaagcgc ccgaataaca ggaaagatgg
    11221 ccatagcatc gccaatctgg tcgggaatat ggatgacaac aaagtctgtt ttttcaatat
    11281 tgaaattata agctttataa tcgtagtaac taaatgcaat acgtctcaac aatgatgcta
    11341 aaaacatacc taacctcgcc tccctactgg ttataatgca atgcagtcta tcagactcat
    11401 cagggtgcca ttttgtgcat atgcggactt ttatgtttca tatctctaac ctgtgggtcc
    11461 tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcttcca tcgaattttt
    11521 catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca
    11581 taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta attataaaaa
    11641 gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc cgatgaaggg
    11701 acgctttcgt taagtcgcgc ccgacgagct gcgttaggca gcttcgctgg tgccgtcgtc
    11761 gactggtatg attttttact ctatggcatc accgccgcac tggtgtttaa tcgcgagttt
    11821 ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgccacctt tggcgtcgga
    11881 ttccttttcc gtccgctcgg cggtgtcatt ttcggtcact ttggcgaccg actgggacgt
    11941 aagcgcatgt taatgctgac cgtctggatg atgggcatcg cgacagcctt gattggtatt
    12001 cttccttcat tctcgaccat tgggtggtgg gcacctattt tgctggtgac actgcgtgcc
    12061 attcagggat ttgccgtcgg cggcgaatgg ggaggcgcgg cgttgctttc cgttgaaagt
    12121 gcaccgaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta cggtgtaggt
    12181 ttactgcttt caaccggact ggtttcattg atcagtatga tgacgactga cgaacagttt
    12241 ttaagctggg gctggcgcat tcctttcctg tttagcatcg tactggtact gggagcattg
    12301 tgggtgcgca atggcatgga ggagtccgcg gaatttgaac aacagcaaca ttatcaagct
    12361 gccgcgaaaa aacgcatccc ggttatcgaa gcgctgttac gacatcccgg tgctttcctg
    12421 aagattattg cgctacgact gtgcgaattg ctgacgatgt acatcgttac tgcctttgca
    12481 cttaattatt caacccagaa tatggggtta ccgcgcgaac ttttcctcaa tattggtttg
    12541 ctggtaggtg gattaagctg cttgacaatt ccctgttttg cctggcttgc cgatcgtttt
    12601 ggtcgccgca gggtttatat cacaggtgcg ttaatcggaa cgttgagcgc atttcctttc
    12661 tttatggcgc ttgaagcaca atctattttc tggatagttt tcttctccat aatgctggca
    12721 aacattgcgc atgacatggt ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt
    12781 gccagttatc gctatagtgg tgctggagtc ggttatcagg tcgccagtgt ggttggcggt
    12841 ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa ctggcatagc
    12901 gtcgccattt atttgctggc tggatgtctg atttccgcaa tgaccgcttt gttgatgaaa
    12961 gacaatcaac gcgcttgata gtctggcgaa gatcatccga tcttcgcctt acacttttgt
    13021 ttcacattcc tgtgacatac tatcggatgt gcggtaattg tatggaacag gagacacaca
    13081 tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc gacgcgctgt
    13141 atgagcaatc tgtagtcgca ttacgcaacg ccattggcaa ctatattaca agtggcgaat
    13201 tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg accgtaacct
    13261 gggacggtag cacaaccaat ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag
    13321 gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctc aatgaacaac
    13381 ttacgttgct gtatcaggat tatggtgcgc atatctcggt gcaaccctcg cagcatgaaa
    13441 tcccctatcc ttatgtcatc gatggctctg aattgacact tgatcgctca atgagcgctg
    13501 ggttaactcg ctacttcccg acaacagaac tggcgcaaat tggcgatgaa actgcagacg
    13561 gcatttatca tccaactgaa ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt
    13621 tttccctcgc gcggttgcgc cattataccg gtacgccagt tgaacatttt cagccgttcg
    13681 tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga tgcagccaga
    13741 tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcaccg
    13801 ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa catcagatgc
    13861 cagcatggca tttaattacc gccgatggtc agggtattac tctggtgaat attggcgtgg
    13921 gaccgtcaaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg gatgtctggt
    13981 tgatgattgg tcactgtggc ggattacgtg aaagtcaggc cattggcgat tatgtacttg
    14041 cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc gatattccta
    14101 ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc caccaagctg gtgagcggca
    14161 ggcccggtga ggaagtcaaa cagcggctac gtactggtac tgtggtaacc acagatgaca
    14221 ggaactggga attacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtag
    14281 caattgatat ggaaagtgca accatcgccg cgcaaggata tcgtttccgc gtgccatacg
    14341 ggacactact gtgtgtttca gataaaccgt tgcatggcga gattaaactt cccggccagg
    14401 ctaaccgttt ttatgaaggc gctatttccg aacatctgca aattggcatt cgggcgatcg
    14461 atttgctgcg cgcagaaggc gaccgactgc attcgcgtaa attacgaacc tttaatgagc
    14521 cgccgttccg ataataagtt ctgaaccttc ttatcagaaa tagatgtgaa ggagtaagta
    14581 agacacctgg caaatagcct gcaataatcg tgggctgttt gcttccttgg gcggatacgt
    14641 gttttattat cgtcttaatg atttccacat attaaaagca agtatgcttt caaaacacaa
    14701 ttataaaaaa tcccgccaac aatataagtt tttataaaat taaatataag attatggctt
    14761 tagaatattt tcatttctaa tagacgagat ttttcctgtt atgatataat atgccgaatt
    14821 aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc attccagtgg
    14881 gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca acgtctaaaa
    14941 tttatgcaaa attcggtgta gaaatctatg ctgctgctaa acaaggtgaa cccgatccag
    15001 aattaaacac atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc
    15061 acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg ttcgtgcagg
    15121 gacgttatga aggctttggt cctaatggct caatgattat cgccgagaca ttgacttcaa
    15181 atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc ggcaatatcg
    15241 gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta tttaaaggat
    15301 cagaccctga ccatattttt gaaattttac ttgaagctga agttgatgtt cgtgatgtga
    15361 ctgaagaaga aggtaacatt gttatttata ccgaacctac tgaccttcat aaaggaatcg
    15421 cggctctaaa agcagctgga atcactgagt tctcaacaac agaattagaa atgattgctc
    15481 aatctgaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt gttgatgccc
    15541 ttgaagatga cgacgatgta caaaaagttt atcataacgt cgcaaatctc taattatatt
    15601 ttaaagaaat ctgtctttac ggcagatttc tttaatctca tataatcctt ataaaaaaat
    15661 ataatgttca actcgtcata tttcttataa aaaatcctcg ttcccagaga aataatattt
    15721 attataattc cagttcttct ttttctgatt acagaaggca aagtgacaat tacgcatagt
    15781 ttcccgataa agacgcgaca gctacatccc gcataaggca tttttctctt tatctttgta
    15841 cggtacttca tggaacagag tttttgacct tgcgaatcgt gatgtctgtt gggggcaact
    15901 tgctcactga agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggt
    15961 cactttttta caaggtaacc gggtaaaaat aatttttatt ttttaactgt tttgagactc
    16021 atagagatgt ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac
    16081 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa
    16141 tattagcgat gcccacccat aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg
    16201 aaataatctg cattttgtcg tttattccga cacagctggc tttttttcac acttttgcgg
    16261 ctcggatcga gggtatttcc atagccaacg tccagtaacc attcgccagt aaaacagcac
    16321 ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag
    16381 catgatgccc agcacataac cgacgacaac ccggcaaccc cacatgctca acatcgaaac
    16441 ccacatggcg taacgggcat cacgagcacc tttaaatcca gcgggtagca cccatgaggc
    16501 ggaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga catgtttaac
    16561 ctgtggatcc tgggtgtaaa acgatgccat aagcccggca aagggagccg ttagccaggc
    16621 gatggccgtt aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg
    16681 cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag agccgagcgc
    16741 acttccgggt aagttgataa gagccgcaat tgaaaacgcg ataaaatttc cggcaataac
    16801 actggtcccc atcccggcaa cgaacatttg ggttaataac cgaccacagg taaataacac
    16861 tgattcgaca ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag
    16921 cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac
    16981 ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac
    17041 aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
    17101 aagaatattc aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag
    17161 tgccccgcta ccaatcaaag tgatggcagc tgctggataa ctgagtaccg tcagctccag
    17221 ataagtcaac gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat
    17281 ttgttcgcca aaatgatgaa taagcgttgc caacagtacg gcaaacaacg tcatgatcac
    17341 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
    17401 cacaacgaca gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa
    17461 gctgtccgcc aatcccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt
    17521 gctcaggaca cccatcaaca ggacacaggc attctccatg aagataggaa cagcaagcgg
    17581 ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc
    17641 gtgaacaacc tggcgtaaaa cagaggagat attcaaagcc gaccttaatt gcagaaagtg
    17701 aaaccacatt tcaaataatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
    17761 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggga atttaaaaat
    17821 cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
    17881 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
    17941 agccaaattt ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat
    18001 ttcctcttca ctgttttcca tcacctgccg cttgatatcc tcaactgaca gtcctgcatt
    18061 acaaagttcc agaaagcgcc agacatagtt acgctgaagt tgtcctcgct tcagtcccaa
    18121 ccagacagta ttagcatcga aaagatgccg cgtatccaga cggattaaat tctcttcctc
    18181 ttgttcgccg ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt
    18241 tttaatgaca tcagaatcct gcgcacttaa tacaatatct gccagcaaac ctttgcgggc
    18301 aaatgcgtca tcaatacgtg agcgccccgt aatcccccgt cggtaagtga ttaacggcca
    18361 cttcgctatt gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac
    18421 aagcaaacta tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt cattactcaa
    18481 acgctcgctg gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg
    18541 tggcgtcccc tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat
    18601 gacctctggc aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga
    18661 cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg
    18721 ttctgcaatg accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg
    18781 tcgaacaaat atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctgacgcc
    18841 tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc
    18901 ctcgcggatt atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatttcc
    18961 cgttattgtt aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca
    19021 ttccgatata acacttagct caccaattgc cactgccttt tttccatcac tggagaacta
    19081 atcactgaca ttaacaactc tttcactgcc tgtgcctgtg gcgataagtt cgctctggcg
    19141 ggtaaattta atgacaaaga gagactcatg gaaggagtgg taatgcgtga catccaccca
    19201 tttactgcgc cacataacga acgcgcggcc gattcgggta atactacaac gcccatgccg
    19261 ctggcaatcg ctgcggtaag cgtggcaata gactcaattt caccaataac ttttgccgtg
    19321 agtcgccgta gggaaaaagc ctcatcaaca cgaagtctaa tagcactgta atcactgggg
    19381 agaaagaggt tcatttgcgc aatagcattc acatcaacgc tttgccccgg acaatcttga
    19441 gttcctacca ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg
    19501 gaatgctcat aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact
    19561 gcaccgctat tttcatgaag atagataacg atctccggga attcagcgcg aaccgcctgt
    19621 aataagggca tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc
    19681 cccgataatg cctgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa
    19741 atggcccgcg catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt
    19801 gtgcgaatca aaagttgttg atttaactca ccttccagtg tggcaacctg ctggctgagc
    19861 gctggttgtg caatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt
    19921 tttacgaagt atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc
    19981 aagtgccgcc attacttaca accagactgc aagatgcttg ccagttttat tttggcgttg
    20041 atgtacaagc taaccaactg tcaaataaga gattatgata gattcgtcat ttgctccttt
    20101 aatcagctgt cgcgttcccc tgccctataa aaggagggta tgcaccacga tgattcatta
    20161 cccaataaga ttgaaagctc accactttgt tgaaattgac agcaaacaaa caaaaaaatg
    20221 catttcaccc tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc
    20281 ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc
    20341 agtcagagga gccaaattca aaaaagcctg ctttctagca ggctttttgc tttctaatta
    20401 ccaacgctct taaaacatct gtcttgaacc agaactaatt tgcacaggca ttcccgatcg
    20461 acgttgcaac gcagcatttg cgcgatttac atcaacttct tgcccgctga taaacgcccg
    20521 caaagatggg gttaccggca atggcacttt tcggtcagac tcatattctg cacgattgcg
    20581 cgacaacggc tcatgaactt ccagccagtt cgagccatct ggttcagtgg tgtattttac
    20641 tggctggtcg ataatttgca cacgcgtccc aacaggaaca ttatcaaaca gatatttgat
    20701 atcgtcattg cgcagacgaa tacagccctg acttacccgg agcccaatac caaaattggc
    20761 attggtacca tggatggcat acaacctgcc aatataaatc gcgtacagcc ccatgggatt
    20821 atcggggccc gcaggaacaa atgcgggcaa actctcccct cgtttcgcat attcgcgccg
    20881 agtgttcggc gttggcgtcc aggttggagc ttcttgttta cgttcaacgg tagtcaccca
    20941 gttacgcggg gtttctcgcc cagcctggcc gataccaata ggaaagactt ccacagtatt
    21001 actgtctggt gggtagtaat aaagacgcat ctcagcgacg ttaaccacaa tccctttacg
    21061 aacagtggcg ggcaaaatca gttgctgcgg aatggtgagt tgcgagccag acttcggcaa
    21121 aaaaacatca gcgcccgggt tcgcttccag catgttactt aacccttgcc cgtattgtgc
    21181 ggcaaaagtc tccagcggct gggtattgtg atcaggaaca gttacagtaa acgactgccc
    21241 cactaaacgg ctaccctctg gaggtaatgg ataagttacc gccaggctag tatggctggc
    21301 aaaaagtaga gcaaatgagc aaaaaatatt tacacgacgc atcatgtccc tttcctaagt
    21361 cgcgaaagct atccgttaag tatagctttt atcagacttt tcgtttttaa ctgttcaaat
    21421 cagaagtcgt attccccggt agaacaatat tactggcagc aagttcgccc atgttgttat
    21481 atatcgcaca ggcagcttcg atgatgggca tcgccagagc tgcgccactc ccctcaccta
    21541 aacgcatctc catattgaga taaggctcca gccccaaatg cgacagcgca atacgcgccc
    21601 ctttttctgc cgacaggtga gaaggaatga gatacggttt gattgcagga gacatctggc
    21661 aggctgcgag cgcggcagca taagaaagaa atccatccag caacacgggt aaaccgcagg
    21721 aagcagcgcc taacatcacg ccagccattc cgaccaaatc aaatccaccc acttttgcca
    21781 ggacatcaac gccatcctgc ggatttggtt gattcaacgt aatcgcccgg cgcacaacat
    21841 caattttatt agccagttta tctgtcggca ggtttgcgcc aatcccaacc acttcttcag
    21901 gagcccggcc agtgattgtg ctgactattg ccgctgccgg tgtcgtgttt gccatcccca
    21961 gttcacctac accaaacagc gtaacaccgt tttttgccag ctcccgcgta taacatatga
    22021 cgtccaaaag caacttttct gcctgatggc gactcattgc cggagctgaa gcaatattgc
    22081 cactacctcg agcaacacgc atgttgataa gcccggggat aggctcagca gtatcaatac
    22141 caacatcaat tacgtagacg ttagcgcccg cttgtgctgc cagcacacac acgccggttg
    22201 ttccgcgggt catattttca gcctgaatgg ctgtcacttc ttttggggaa atagcgaccc
    22261 cttcctccca gacgccgtga tcggcacaca taaccagtac cgcttttttg cccacatgcg
    22321 gtatgccatt caaccccggc attcccgcca gttgtatggc aagcgcctcc agccttccca
    22381 ggctaccaac aggtttgagt aacccgtcaa tatgccgttg tgcacgcgac atagcggcag
    22441 gatcgatagc agggatcgta ttcagtaaat cggcaagtgt ttgcatgtca cgtctcttta
    22501 tagcagtgcc agcaggaaga ccagttcacc aagctctata gcagctccca gcgtatcgcc
    22561 ggtttgcccg cccagcgtac gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt
    22621 caccaccata gcagccacac catgcatacc cggcaataat acggcagcaa aaatagcggc
    22681 taagccgagt gtgacgcagg tttgtcgccc atcaattttg ccaatgaaca cattgccaag
    22741 tccttcttcc cgggcataac gatgacgata catcaataat gcggcagtcc cgcggctgac
    22801 cgcacatgct gccgccagcg atgcaaggat cggttcgcca cgcagagcca actcgcttaa
    22861 taccagaatc tttgccagta caacaaaaat caatgccaga ccgccgtagg tgcctaaacg
    22921 gctatcacgc atgatttcca acatgcgatc gcggctacgt gcagaaaata cgccatcaca
    22981 ggtatcggcg aggccgtcca ggtggaatcc cccggtcatc agcgccagca caagtacgct
    23041 aaacagtgcc gccagtggta cgccacacca tgcctgcagc accatgaaga ccagcccgct
    23101 aatcgcgcca agcaataatc caattaaagg aaaagtaata ataccgcgag aataatgctc
    23161 gaaatccagt ccctgggacc agcgacgcgg tacaggcaga cgcgtaataa aagagagcat
    23221 cgcccaaaat aatttactca tttgattttt actccaatac ccgaaaccac caaccatact
    23281 tcatttgccg cagcggccag ctgctgattt acccgcccgg caatatcacg aaaatgtcgt
    23341 gccagacgac tctccggcac aatccccatt cccacttcgt tagtcactaa tacaaccttt
    23401 gcggggcaac gttggcaggc agcaatcaac gactgaatct cagcattaat cgcctgttcc
    23461 atcgcctgat aatcccattc atcagggtct ttatcgccgc cataatcaaa caacagatta
    23521 gtcaccattg tggtaacgca ttccagcaac acagcttcat gcgggttaat gtctgcatga
    23581 attaattcat caacatgttg ccaacgctcc accgtgcgcc agtgcgccgg acggccttgc
    23641 cgatgatgtt ctatccgtgc agacatctca tcatcaagga tttgcgaggt ggcgatatac
    23701 agaacctgtg aagagtcccc aataagcgcc tctgcgtggc gactcttccc gctccgtgct
    23761 ccacctgtca ccaaaatcat cataccgact cctgattcaa ttgtatgatg gtattcattg
    23821 aacagaacaa tggatccccc accgttgctg accgcaaaac atttacgcga ttaatactgc
    23881 gcgtaatata attaaaaatc cagcattctc aatccattac gaaagaagat ggcattgtgg
    23941 aatagccaga cttcctcgtg ttaacgcccg gtcattggag tagtaaccaa agcagattta
    24001 gccaacatgg aacaaatatc tttggtgaaa agttggttac gggaagctgg agcgcacaac
    24061 gtgttagtaa ccagtgcagt taataacaat ggagttacag aactttttgc cttgctgcat
    24121 acagaagaag gctgttgtta atttcgattg gttctcagat gagaatgatg acggtgatac
    24181 acagataaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacggga
    24241 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    24301 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    24361 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    24421 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    24481 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    24541 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    24601 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    24661 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    24721 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    24781 actcaagctg atgtgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    24841 caaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    24901 atttttccgt attggcaaaa accggctgcg gattaccttc gtacaacgca tgaacaaggc
    24961 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    25021 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    25081 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    25141 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    25201 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    25261 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    25321 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    25381 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    25441 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacata gccgccgcgc
    25501 accagactaa catcagcggt aacgagatta actccagttg cagagcgata agacctaata
    25561 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcgcg agggacaacg ttaccccaga
    25621 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtactt tccgggatag
    25681 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    25741 caatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    25801 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    25861 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    25921 aatttgaaag gcgcatctta ctcttttcgc tttcaaaaaa aagagtggtc atcgcgttaa
    25981 cacaccgccc tgagatgaat tagtgatgtt ttggctgcac cgcaacatac atttcgatat
    26041 cccagtaccc atcttccgcg ccattgttca aataaacctc gaagcagggc tttggtaaca
    26101 tttcataagc actgtcctgc aagaggctat taaagaactg ataccagggt ttagcaaaat
    26161 catcaccgac tacacgagcc accgcaaccg catactgacc acctgaaatt tctgtcagaa
    26221 tgacgccctc actgttttcg gggagcgtaa agttattcgg taccgtcacg acggtgtcgc
    26281 agcgtaattt ttcggcgggt gtttcatctg gattgtcgta atagacagca acccactcct
    26341 tcggcacaat attttggcta tctacccaca tcatcaactg ctcaaaacct ttctttaccg
    26401 tctgttccca cgggccaacg agatggaaac ctgcaacggt acgtttatct tcctgcttaa
    26461 tctcgtagtt catgacgcct ccattaatac tgtttttata tacagtatag ttgcaatatt
    26521 aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc cagtctgctg
    26581 caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgagaaaa catgctgcct
    26641 tccccgacag attccagggt aaccagcggc cagtgcgcca cctgtttatc acggtcgtaa
    26701 agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagttc tttaccatca
    26761 agggtatatt tggctttgat atgtggaatt tcggctttcg gtagcaccat ccagaactct
    26821 tgttccgttc ccagggcgat attttcttta tcgccatacc agatgcgttc cgtaccgacc
    26881 tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgccccca acgcagtaat
    26941 tttcttgcct cttcctcacg accttttgcg ctgtcagcac ccattaccac tgcaatgaga
    27001 cgacgctgcc catctacagc cgaagcaatg agattaaacc cggcaccaga agtatgaccc
    27061 gttttcaggc cgtcaacatt catggtttta tcccacaata acccgttacg gttttgctgg
    27121 gtgataccgt tccaggtgag gcttttctca ctgtacatat gataaaactc gggctcgccg
    27181 tggatgatag cacgagaaag cacagctaaa tcataagccg agctatgctg acctggcgca
    27241 tccaggccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc ggcatagttg
    27301 ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag agcaacacaa
    27361 gcgtcatttc cggaatcaac aattaaacca cggcttaaat cacgtaccga tacgcgatcg
    27421 ccctctttca aaaacatcag tgaagaaccg acaaacaccg gattatcttt cgcccacgca
    27481 tcgcgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat agcgcgatcc
    27541 acgacatagc ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg atgctcatta
    27601 cccgcggtga gaatctgacc ggtggtgtaa tccatcagta cccaggaccc ggcttgaatc
    27661 tgtggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga taagttaaaa
    27721 acgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc aggagtttca
    27781 aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg caagaaaatg
    27841 tgactactct ctcattttta tctgacatga tctgttgcca ctcgctgtca aattgtcgcg
    27901 ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa taacggattt
    27961 aacctaatga tgaatgacgg taagcaacaa tctacctttt tgtttcacga ttacgaaacc
    28021 tttggcacgc accccgcgtt agatcgccct gcacagttcg cagccattcg caccgatagc
    28081 gaattcaatg tcatcggcga acccgaagtc ttttactgca agcccgcgga tgactattta
    28141 ccccagcctg gagcagtatt aattaccggt attaccccgc aggaagcacg ggcgaaagga
    28201 gaaaacgaag ccgcgtttgc cgcccgtatt cactcgcttt ttaccgtacc gaagacctgt
    28261 attctgggct acaacaatgt gcgtttcgac gacgaagtca cacgcaacgt tttttatcgt
    28321 aatttctacg atccttacgc ctggagctgg cagcatgata actcgcgctg ggatttactg
    28381 gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa tacactggcc tgaaaatgat
    28441 gacggtctac cgagctttcg ccttgagcat ttaaccaaag cgaatggtat tgaacatagc
    28501 aacgcccacg atgcgatggc tgatgtgtac gccactattg cgatggcaaa gctggtaaaa
    28561 acgcgtcagc cacgcctgtt tgattatctc tttacccatc gtaataaaca caaactgatg
    28621 gcgttgatcg atgttccgca gatgaaaccc ctggtgcacg tttccggaat gtttggagca
    28681 tggcgcggca ataccagctg ggtggcaccg ctggcgtggc atcctgaaaa tcgcaatgcc
    28741 gtaattatgg tggatttggc aggagacatt tcgccattac tggaactgga tagcgacaca
    28801 ttgcgcgagc gtttatatac cgcaaaaacc gatcttggcg ataacgccgc cgttccggtt
    28861 aagctggtgc atatcaataa atgtccggtg ctggcccagg cgaatacgct acgcccggaa
    28921 gatgccgacc gactgggaat taatcgtcag cattgcctcg ataacctgaa aattctgcgt
    28981 gaaaatccgc aagtgcgcga aaaagtggtg gcgatattcg cggaagccga accgtttacg
    29041 ccttcagata acgtggatgc acagctttat aacggctttt tcagtgacgc cgatcgtgca
    29101 gcaatgaaaa ttgtgctgga aaccgagccg cgtaatttac cggcactgga tatcactttt
    29161 gtcgataaac ggattgaaaa gctgttgttc aattatcggg cgcgcaactt cccggggacg
    29221 ctggattatg ccgagcagca acgctggctg gagcaccgcc gccaggtctt cacgccagag
    29281 tttttacagg gttatgcaga tgaattgcag atgctggcac aacaatatgc cgatgacaaa
    29341 gagaaagtgg cgctgttaaa agcgctttgg cagtacgcgg aagagatcgt ctaatttgaa
    29401 gcctccgccg ctggtacggc ggaggacaat gttgagtcag gctttttgaa cggtgatgct
    29461 ccacgctgca tcgccaatct gctgataatc ggtgatggca tgtccttctt ctgccgccca
    29521 ctgtgggatt gcttcagttg cctgggtgca atcaaactca attaccaact catcgccgct
    29581 ggccatttct gccagtgctg cttttgcttc tatgagtgga aacggacaga cctgcgtcac
    29641 cacatccagc tttttaattg ccatctttac accttactta atttgccgca gcagttgcca
    29701 gtcgcgcctt acgctgcggt cgaacataca caagccagga cgcagtccag actccgagaa
    29761 tcataaatac aaggccaatc cagccctgcc aggtcatcat tgcagtcata accagtccat
    29821 taccgataga gcaaccacct gcaatactgg caccgaagcc catcagtaca ccacctaacc
    29881 cgctacgtaa tgttgtttgt gcatcagctg cgcgaacgcg gaactcacgg ctcgctttgg
    29941 ctgcaataaa tgaccccacg aagatcccta acaccaggaa aacgccccag ttaatgtatt
    30001 tcacgtcacc cgcgaccaga aattgcagaa tattggccgt tggggaagtg attcctaacc
    30061 caaacatgcg tccggttgct tcactcaggg gccaggctaa aagcgcaatc aaaccgatga
    30121 gtacagcagt gacaaaggga tgccagcgtt tttcaaacag aatatgagcg atcccggttc
    30181 ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt caactctttc atcaccaccc
    30241 agagcgttat caccagcaaa acggcaacca acggccacac agacaaatta aaagtctcag
    30301 caatagagtt atgttcagta ctgtagtgct gcaaggtttg atttaaacca ctggcatgtg
    30361 gagaacgcat caccgcactc atcaccatat aagtgaaaag cgcgatccaa ctgccgatca
    30421 atccctcacc cgcgcgatac caggtcccgg tggcacatcc acccgccaga acaattccca
    30481 gcccgaagat atacccacct ataacagtac caagccacgg gaacgccccg gcttcgtaag
    30541 tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct ttgtaccgaa attgcaatca
    30601 gcaaggcgta aaacatgcga ttatttttca cgatatacat atcgcgaaaa ccacctgtca
    30661 ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc accacaaatt agcccgctta
    30721 atatcattga aaacataaat taattaacca gatgaatgtt aatggggaaa ttattcatga
    30781 ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt tattaatgag
    30841 gatttcgata aaaaagaata aaaaaaccgg agccaaatgt tcattcgact ccggtttatt
    30901 attagaaaag atgattatgc tacgtcttcg tactgcggaa ccgggttacg gaaactcttg
    30961 gttacgcaag ccaggtaaat cagaccgata gcagcccaga tcagacccag aaccattgag
    31021 ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac
    31081 agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca gaactgcgaa
    31141 ataaccgaca ggttaacgaa ggtgaacgcc accagcgcac caaagttaat cagcgccgta
    31201 gccattacca ggtcgaagtt gattgccagc aaggcaatcg cgccaaccag gatgatgttc
    31261 attgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaacacaccg
    31321 tcacgcccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat accggacgcc
    31381 agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac
    31441 agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac
    31501 agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagcgc ggtcaggaaa
    31561 atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg
    31621 tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc
    31681 gcatcgccag accagaacgg acgagtgctc gccagcgtac ccgcgccttc accttcaaat
    31741 acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt
    31801 acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt cataaaggcc
    31861 accagcgcca ccacaaacat ccacgatggg atggaaggca ccagagcttc aaaatagatt
    31921 ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc
    31981 accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca
    32041 gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat
    32101 gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaaagta
    32161 tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg aactaacgta
    32221 agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt
    32281 ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc
    32341 agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg
    32401 tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg gtttttagtc
    32461 attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc
    32521 ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac
    32581 cggcgatcgc actatttgct aaaatttcat ctcgccacca gccgctggca agccctgcgg
    32641 tctgttcgtt ccagccaatc cagacgggtt caaagagagt ttgggcgact actttcttct
    32701 cgattaacgc gccactatcg agaaaacgtt gcgccagata acggggtaaa tagccgcagc
    32761 caaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atggcttctt
    32821 gttcgtcgag aagttgtggg gccgtagaag cgccggcctg cgcagtgtcc cccaccacaa
    32881 tcgcccgata tcgtttaatg atacgacggt ttaacggttc ttcttccagg gctaacggat
    32941 gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgtgaaaaa ccaaactcac
    33001 tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcaggg
    33061 catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg ctatgatgtt
    33121 gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa
    33181 tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc agttcccgca
    33241 cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtacgg gtgaatttag
    33301 cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg
    33361 tgtaactcag agcggacggc gtcttataga gtttggccga cgccgccgca aaactgcctt
    33421 ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata cacgccccct
    33481 tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat
    33541 cgggatactg gtattcaaac cccagttcat tacaaatccg actgccatca ataatcttac
    33601 ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt aaacgggcca
    33661 tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc
    33721 cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct aaatggacta
    33781 aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt ccgggatgac
    33841 gtcccggtcc gaccaggccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt
    33901 gcagccagtc ttcgagttct tctaatactc gtccactgtt ggttaccgga ttacgcgagg
    33961 tggtttcttt caccgtgcct tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa
    34021 ttcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga
    34081 actcatcgcc ggagccgcta cgacgtgccg gaagcgtaat gaccagtgca tcggcatcca
    34141 tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ttccatgcga agcagatagc
    34201 tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga
    34261 cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat acctaaccac cctaacccga
    34321 caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata aggctacgct
    34381 agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat
    34441 gctgcaatga gcagtgaatc atcagtaacc gcgtatacca gacggtgctc ctctgtaatg
    34501 cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggtttccc cttaccttca
    34561 aatggcgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct
    34621 gtttcctgcc agtacagata atcatcccat gattcctcag accagattag tttcactcaa
    34681 taatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc atcaatctcc
    34741 gggcgttagc gggggaacgc agtagataag ccgtctcttc cagcgagttg tattcttcga
    34801 gtgacatcag aacacaagcc tctccattct gacgagtaat gaggatcggg gcatgatctt
    34861 caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtaa ctaattgtac
    34921 gcatgtcaat ctcctctttt gtacagctta ttgtacaatg atagacgttg attcactatt
    34981 tattaattag tttgtagatc aagacattgt cagtgagacg aaaaatccag gcttcgctat
    35041 tttgggtacc gtcaggttag aggacagtcc tcttagcgcc ctcctttccc cgctcattca
    35101 ttaaacaaat ccatcgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
    35161 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
    35221 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
    35281 aagacattca gatcttccag tggtgcatga acgcatgaga gagcccccgg aagatcacct
    35341 tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag
    35401 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
    35461 gactcacgcg aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg
    35521 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
    35581 ggtctggtaa tggacggcgt ggtagacctt ggaattatcg gcgaaaacgt gctggaagaa
    35641 gaactgctta accgccgcgc ccagggtgaa gatccgcgct actttaccct gcgtcgactg
    35701 gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg ggacggcccg
    35761 ctctccttaa atggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
    35821 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg
    35881 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagct
    35941 aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgcctgat ccagcgcgat
    36001 ggcgaaatgg aagaatccaa acagcaacta atcgacaaac tgctgacccg tattcagggt
    36061 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat
    36121 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacta ttctgccgct ggcgggtgac
    36181 caacagcgcg tagcgatgca catggtcagc agcgaaaccc tgttctggga aaccatggaa
    36241 aaactgaaag cgctgggtgc cagttcaatt ctggtgctgc cgattgagaa gatgatggag
    36301 tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
    36361 gccagctgtt aatgcgcccg gcgatttccg cctctgaaag cattacccgc actgttaacg
    36421 atattctcga taacgtgaaa gcacgcggcg atgaggccct gcgggaatac agcgcgaagt
    36481 ttgataaaac cacggttacc gcgctgaagg tgtctgcaga ggagatcgcc gccgccagcg
    36541 aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct
    36601 tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc
    36661 agcaggtcac gcgtccggta gcttcagttg ggttgtatat tcctggcggc tccgccccgc
    36721 tcttctcaac ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt aaaaaagtgg
    36781 tgctgtgctc accgccgccg attgctgatg agatccttta tgcggcgcag ttgtgcggtg
    36841 tacaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg tttggtacag
    36901 aatctgtgcc gaaagtggac aaaatcttcg ggccgggcaa cgcctttgtc accgaagcga
    36961 aacgtcaggt gagccagcgt ctggacggtg cggcgatcga tatgcccgcc ggtccgtcgg
    37021 aagtgctggt gattgccgac agcggcgcaa cgccggattt cgtggcctct gatttgctct
    37081 cccaggcaga acacggcccg gactctcagg tgattttact gacgcccgat gctgatatgg
    37141 cgcgtcgtgt tgccgaagct gtcgcacgcc agctggcggt actgccgaga gctgaaaccg
    37201 cccgtcaggc actgagcgcc agccgcctga ttgtgaccaa cgatttagcg cagtgcgtgg
    37261 agatctccaa ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc
    37321 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg
    37381 caggtgatta cgcctccggc accaaccacg tactgccgac ttacggttat accgccacct
    37441 gttccagcct cgggctggca gatttccaga agcgtatgac cgtgcaggaa ctgtcgaaag
    37501 aaggcttctc cgcgctggct tcaaccattg aaacactggc cgccgccgag cgcctgaccg
    37561 cccacaaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
    37621 gactatcacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg
    37681 tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatatccca cagccgtgga
    37741 gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgccagccga aagtggtgat
    37801 tgaaaattac gcgcaatatg caggcgtaaa accggagcag gtgctggtca gccgtggcgc
    37861 ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
    37921 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgcc gaaacgattg gcgtcgagtg
    37981 ccgcacagtg ccgacgctgg acaactggca actggactta caaggcattt ccgacaagct
    38041 ggatggcgta aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa
    38101 tccgcaggat tttcgcaccc tgctggagtt aacccgcggt aaggcgattg tggttgccga
    38161 tgaagcctat atagagtttt gcccgcaggc atcgctggct ggctggctgg cggaatatcc
    38221 gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcggggc ttcgttgcgg
    38281 atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg ccccctaccc
    38341 gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc
    38401 catgcgtgag cgggtggcgc aaattattgc agaacgcgaa tacctgattg ccgcactgaa
    38461 agagatcccc tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt
    38521 taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
    38581 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
    38641 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
    38701 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
    38761 gacaaactcg cctttgaacc gggcgtgatt ccggagctgc tgaagctgca aaaagcgggc
    38821 tacaaactgg tgatgatcac taatcaggat ggtctgggaa cacaaagttt cccgcaggcg
    38881 gattttgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt
    38941 gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg taagccgaaa
    39001 gtaaaactgg tggagcgtta tctcgctgag caagcgatgg atcgcgctaa cagttatgtg
    39061 attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa tggtttacgc
    39121 tacgaccgcg aaatcctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt
    39181 tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt gtggctggat
    39241 cgcgaaggtg gcagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag
    39301 atcgccaccc acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac
    39361 gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa aattgctctt
    39421 ggcgacaaac gcggtatttg ccgcttcggt tttgtgctgc cgatggacga atgccttgcc
    39481 cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagctga atttacctac
    39541 cagcgcgtgg gcgatctcag cactgaaatg atcgagcact tcttccgttc gctctcatac
    39601 accatgggcg tgacgctgca cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag
    39661 agcctgttca aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgacacc
    39721 ctgccctcgt caaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca
    39781 atctgaactc ggtaaagtct gccattgcgc gccacggtta tgaaccaaaa gtcagccgtg
    39841 acccagacgt cgtgttgctg gccgataaac tatttttacc cggcgttggc accgcgcaag
    39901 aagcgatgga tcaggtgcgt gagcgcgacc tgtttgatct catcaaagcc tgtacccaac
    39961 cggtgctggg catctgctta gggatgcaac tgctggggcg gcgcagcgaa gagagcaacg
    40021 gcgtcgattt gctgggcatc atcgacgaag acgtgccgaa aatgaccgac tttggtctgc
    40081 cgctgccaca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg
    40141 ggattgaaga cggcgcgtac ttttactttg ttcacagtta cgcaatgccg gtcaatccgt
    40201 ggaccatcgc ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact
    40261 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa
    40321 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
    40381 tctccatcag ggcgattacg gcaaacaacg tgattacggt aacgacccgc tgccgcgctt
    40441 acaggattac gccgcacaag gtgccgaagt gttgcatctg gtggatctga ccggggcaaa
    40501 agatccggct aaacgtcaaa tcccgctgat taaaactctg gtggcgggcg ttaacgttcc
    40561 ggtgcaggtt ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg
    40621 cgttgcgcgc gtagtggtcg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg
    40681 gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca
    40741 aggcaacaag caggtggcag tcagcggctg gcaagagaac tcgggcgttt cactggaaca
    40801 actggtggaa acctatttgc ccgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg
    40861 cgacggcacg ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc
    40921 gcaggtggca tttcagtcat ccggcggcat tggcgacatt aacgatgttg ctgccctgcg
    40981 tggcactggc gtgcgcagcg taatagttgg tcgtgcatta ctggaaggta aattcaccgt
    41041 gaaggaggcc atcgcatgct ggcaaaacgc ataattccgt gtctcgacgt tcgtgatggt
    41101 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgatat tgtaccgctg
    41161 gcaaaacgct acgctgaaga aggcgctgac gaactggtgt tctacgatat caccgcttcc
    41221 agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt gatcgacatt
    41281 ccgttctgtg tggcgggtgg gattaaatcg ctggatgacg ccgccaaaat tctttccttt
    41341 ggcgcggata aaatctccat taactcccct gcgctggcgg acccgacgtt aattactcgc
    41401 ctggccgatc gctttggcgt gcagtgtatt gtggtcggta ttgatacctg gtacgacgcc
    41461 gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgttact
    41521 cagtgggaaa cactcgagtg ggtacaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
    41581 ctcaacatga tgaatcagga cggcgtgcgt aacggttacg atctcgaaca actgaaaaaa
    41641 gtgcgtgaag tttgccacgt cccgctgatt gcctccggtg gcgcaggcac catggaacac
    41701 ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggcagcttc tgtattccac
    41761 aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
    41821 aggatatgtt aacagaacaa caatgtcgcg aactggactg ggaaaaaacc gacggactga
    41881 tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc
    41941 cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
    42001 agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caacgtagta aatattacgc
    42061 cggactgcga caacgacacg ttactgttgc tggcgaatcc catcggcccg acctgccaca
    42121 aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg
    42181 aacaactgct cgccgaacgt aaatctgccg atccggaaac ctcctacacc gccaaactgt
    42241 atgccagcgg caccaagcgc attgcgcaga aagtgggcga agaaggcgtg gaaactgcgc
    42301 tggcagcaac ggtacatgac cgctttgaac tcaccaacga ggcgtctgac ttgatgtatc
    42361 acctgctggt gctgttgcag gatcaggggc tggatttaac gacggtaatt gagaacctgc
    42421 gtaaacggca tcagtgagtt gcggggtaag cggatgcgat attgttgtcg catccggagc
    42481 catagcccga tgcgcaagct tatcgggccg actattctct ctgcacacgt aagctggata
    42541 catcgcatcc ggcaaaaaaa acgggcaagg tgtcaccacc ctgcccgttt tctttaaagc
    42601 tgcaaagatt atttcgcgtt gtaattacgc agcgcattac gccccagcac aatccccgcg
    42661 ccaaccatgc cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg
    42721 cgacgaatag gtaacatcgg tttcatcaca taacggtaag catgaatatc aagatcatca
    42781 acctttaagc tttcgatatc cagcaggttt tgacgagtct gatagtagtt tggcgagaac
    42841 accaacggac gggtcgcctc atgcttaatc atcgactcca gcgcttcgct ccccaaaagg
    42901 aacaacgtat cttgtgtcac atcttcagtc tgctgaactt gtggctttgt cacctgcgcc
    42961 tgattcgcat actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc
    43021 gcgaccactt cctgggtttt cagagagtcc tgcaagtttt tcatacgcag cgcgatgtta
    43081 tctttcaggt ctttttccag ttcttcattg acctggtcgt caacttgctg gatatacttc
    43141 gccagctgtt tctgcgcacc ttcaggtgtt tcacccacat aagaaaccgc taacggtaat
    43201 gactgatttt taacggtagg ctcaatggtc agcttttctg cttcttcctg gttatccagc
    43261 gtttccgcta atgctgagaa tgcagtgctg taacgtccga taagtgaagc ctgaatgtcg
    43321 gagactttcg gtacagctga tccgtagatc acattcatcg cgttggtata accagcgatt
    43381 tgccccacat caggttgagt aacgatagct gtggaagtcc acttttcttt ggcaacggcc
    43441 agatacccta tagccagggc aatagccaca atgacagaaa tgatgatagt catcttgccg
    43501 cgccatagtt ggaccagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca
    43561 gaaacattat tattttctac tctcatcatt accctaacgg acgaaataat tttaagtcgc
    43621 catagtttag cgataatcgc catgaatgct ataggataaa tgataaaaat aatgaattat
    43681 tacaaaggga acataagaga atcagggcga aaatcgccct gataacaaga tgttagtcgc
    43741 taccaaagag atcgcgggtg tagactttat ctgccacatc cttaagctct tctgccattc
    43801 ggttggaaat aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccagacgag
    43861 agttgaagaa tgtgtcttct ttcatcaccg gctcgtagat gattacttca acacctttcg
    43921 ccttgatacg tttcataatt ccctgaatgg aagacgcacg gaagttatct gaaccgctct
    43981 tcataatcag acgataaata cccaccactt gcggtttgcg tgacaaaatg gcatcggcaa
    44041 taaaatcttt acgcgtgcgg tttgcatcga caattgccga gatcaggtta ttcggcacag
    44101 actggtagtt cgccagtaac tgcttggtat ctttcggcaa acaatagcca ccataaccaa
    44161 acgacggatt gttgtaatgg ttgccaatac gcggatcgag acaaacacct tcgataatct
    44221 gacgagtatt cagacctaaa ctttctgcat agctatccag ttcattaaag tatgctacgc
    44281 gcatcgccag ataggtatta gcaaacagtt taatcgcttc tgcttcggtt gaatcggtga
    44341 acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa taacgccgca aaacgttctg
    44401 cgcgttccga acgctcacca atgacaatac gtgacggatg aaggttatcg taaagagctt
    44461 taccttcacg gaggaattcg ggggagaaaa taatattttc agtacgatat ttcttatgca
    44521 tcgctgcggt aaaaccaacg ggaaccgtgg atttgatgac cataaccgca taaggattta
    44581 tctctactac gtctttaatt actgattcta cactggatgt attaaaataa ttagttttag
    44641 gatcatagtc ggttggagtg gcgatgatga cataatcagc atcctggtag gcttcatttg
    44701 tatctaatgt ggcattaaag tgtattttat ctgattgcaa aaactgctga atttccttat
    44761 caacaatagg agatatccga tcattcagca tagcaacgcg tgacggtaaa atatctaatg
    44821 ccacaacctc atgattttgt gcgattagaa gcccgtttga caagcctaca tagccagtac
    44881 cggaaatggt gatattcatt tcatgctctc agaattaact taactgtgaa tcatgatgtt
    44941 tttagcatcc tgataagagt taaaagtttt aacgctacgt cgatagctgg tcaacgcaac
    45001 gaaaaacaat tcagattttc tttataagaa tagctgatat ttattaagtt aatattaagc
    45061 agtgaaattt agtctataag ataattggca aaaaaaagcc cgatgcaata tacgccgggc
    45121 ttcaatttta ttgttggtta aatcagatta atccagccat tcagtatgga acacaccttc
    45181 tttatcagta cgcttatagg tatgcgcacc aaagtagtca cgttgagcct ggatcaggtt
    45241 agccggcaat acggctgcac ggtagctgtc gtagtaggca acggcagcag agaaggtcgg
    45301 taccgggatg ccattttgaa ccgcgtaagc aacaacatca cgtaacgcct gctgatactc
    45361 atcagcgata ttcttgaagt acggatccag cagcaggtta gcaatacccg cattcttcgc
    45421 gtaggcatcg gtgatcttct gcaggaactg cgcacgaata atgcagccgg cacggaaaat
    45481 cttcgcgatc tcaccgtaat tcagatccca gtggtactcg tcagacgctg cgcgcaactg
    45541 agagaaacct tgtgcatagg aaacaatttt accgagatac agcgcacggc gtactttctc
    45601 aataaactca actttgtcac ccgcagggtg cgctttcgga ccagtcagga ccttagaggc
    45661 agcaacgcgc tgatctttca gtgaggagat gtaacgcgca aagaccgatt cagtaatcag
    45721 cgacagcggt tcaccgagat ccaaagagct ctggctggtc cacttaccgg tacctttgtt
    45781 agccgcttcg tccagaatga catcaacaag gtatttaccc tcttcatctt ttttggtgaa
    45841 gatgtctttt gtgatgtcaa tcaggtagct gctcaactca ccgttgttcc actcagtaaa
    45901 ggtactggcc agctcttcat tagagaggtt caggccgcct ttcagcagag aataggcctc
    45961 agcgataagt tgcatgtcac catattcaat accgttgtga accatcttca catagtggcc
    46021 tgcaccatcc gcaccgatgt aagtcacgca aggctcgcca tcttcagcaa ctgcggcgat
    46081 cttagtcaaa atcggcgcaa ccagctcgta ggcttctttc tgaccgcccg gcatgataga
    46141 aggccctttc agcgccccct cttcaccacc ggaaacgccg gtgccaatga agttgaaacc
    46201 ttcagcggaa agttcgcggt tacgacgaat ggtgtcctgg aagaaagtat tgccaccatc
    46261 aataatgatg tcgcctttat ccagatacgg tttcagagaa tcaatggctg catcggtacc
    46321 cgcgcctgct ttcaccatta acaggatacg acgaggcgtt tcgagggatt caacgaactc
    46381 tttcaccgtg tagtaaggca ccagtttctt gcccgggttc tcggcaatca cttcttcggt
    46441 cttttcacgg gagcggttga acacggagac ggtataacca cggctctcga tgttgagcgc
    46501 caggttgcgc cccatcactg ccatgccgac gacgccgatc tgttgcttgg acattacata
    46561 ctcctgtcag gtgtggtcac cgcgcgacgt atgcgcggct tgaatgtata gcaaatgtta
    46621 acctatgtcc gtttgaaaaa aaacgaaaat caatttaacg aatgtcggaa ataggtaacc
    46681 agttgctgtt tcataacata tgctgcgctc cgataaatac ttattctctg ttattcactt
    46741 tccaattaat tgaaataaat attgaccata ctgtgttttc tccataatcc tggcctcagt
    46801 caggacctgc tccgcagtca gccatttatt gcgcaaagct atttcttcta agcaagcaac
    46861 tttatatcct tgacgctttt cgatagtatg aacaaattga gaagcttcaa taaggctatc
    46921 atgtgttccc gtatccaacc aagcgaaccc ccttcccaag agttctacat taagcatatc
    46981 ccgctcaaga tacattttat ttattgaagt gatttcaagc tcaccacgag gagatggttt
    47041 aacttgcttt gcaaaatcca ccacctgatt atcatagaaa tagagtccag ttatggccca
    47101 attagatttt ggtgtttgtg gcttctcttc gacagagagc accttaaatg attcgtcaaa
    47161 ttctacaatg ccaaatcgtt caggatccat aacctgatag ccaaaaactg tagctcctct
    47221 ttttctggaa gccacctgtt ccagtttgcg accgaaactt tgaccgaaga aaatattatc
    47281 ccctaaaacc agggcacatt catcaccagc gatgaactct tcaccaataa taaaagcctg
    47341 cgccaaccca tcaggacttg gttgaatagc atattgaagg ttaattccaa accgggatcc
    47401 gtctcctagc aggcgtttat acgatgataa gtcctcggga gtagaaataa gaagtatatc
    47461 ttttatccct gccagcatta atactgaaat agggtaatat accattggct tatcataaat
    47521 aggtaggagc tgttttgata cccctcgagt aatcggataa agtcgggtac cagaacctcc
    47581 cgctaatata attcctttca ttgcttacct tctctatata atcttcaaat attatcgcat
    47641 taacttttaa gtaaatttta aaaatgcagt gcaataaaca ttcagtttaa ataagcttga
    47701 cattagaaat atctccagct tatttacttg tattaaatgt cacatttaat ctaatttttt
    47761 taggtatgtc gctggagtgc cgcctactaa ttccttttca ccgacatctt ttagaactac
    47821 agaatttgct gcaactatag ctccattgtt aataacaata ggtcccaaaa taacagatcc
    47881 agtggccaat ataacatcgt tgccaataac aggacgaagt tccttgttat atttcatatc
    47941 aagtgttttt gcaccaatag tgacattttg atatatagtg cagttttcac ctatacgcgc
    48001 cgcaccgata actatgccaa aggtatgtgg tatgaaaagt ccacctttaa tctcacattc
    48061 agttgcgatc tcaatgttaa aaatgaggac gtttaaaagt gaacaacatt ttgatattct
    48121 tctgaatcct ttttctttta aagaatagga tgtcctatat attacattgg gaatcaaacg
    48181 agggctaaaa attctaaaat agtctttaaa tttaaaagca gattcattcc caatcaattc
    48241 tttatatctt tgaatatctt gttgtatatt tttcattttt tattcctgcg tatcctgcta
    48301 aaaacaccag atatagccaa aacataggga aaaggaaggt tagtttaagg caggggagca
    48361 ggcataataa catcaatcta agtgagaatc gtgaatattt taggaacaat accagccatg
    48421 atacactaag gatactgcct aaccaaaagc caaaaacgat gaaattactt ccgaataacg
    48481 tcatatcaaa aagagactga aaatagtctg gtgcattgtc ataataccca accataccgt
    48541 ggccaatatt aaaaacaaga gggctatcaa agaatgattt catcaactca agtttatatg
    48601 atgtgctacc atcactacca gatgaaatat aagacatacg ccacataatg ttttgatata
    48661 ttatcggaga aaccagtact aacgatatta acccggttgc gatccacttt agtgtcaatc
    48721 tacaggtaag aataaaaaat aacataacgc agataacaga gaaagtcgaa ccggataaca
    48781 ccatacacat taatgctata taacttagta atgagttatt attagctaca atatacctca
    48841 tcactaataa tgtgaccata tgtatagaga aaatactcgg ttcggcaaat actcctgttg
    48901 ctctgtaatc gaaaccgtca taactgctac ggtgtggagg acctaacaag aacttcccat
    48961 aatcgatatc cacattaatg attttgaaag ctaaatactg caacaggaaa gtaaaaatgt
    49021 gaaacttgat gcaaaaatcg atcaccttaa gccagtcaca gcttttaatt ctttttacac
    49081 aatagctata gattgatggt actgtcaatg cataaaagca gcataccaaa gtaaaaataa
    49141 atattttatc cactacgcca aagaatacat gaaaaataag cgatgtgaac gataccagaa
    49201 taaatatcaa cccttgcgat gaaaccttac atttaccctg tataaacacc agaaaaaaaa
    49261 cgcaaaactg aataataaaa aaaaccggac taaaaatgaa aagagtacca aaaagaaaat
    49321 atatattagc catttctttc cctttttgaa taaccctcta tataatacat agccaagact
    49381 aacttgtaaa atattgctct gattttaaat ttattcctat tgtctaactt attaagaaca
    49441 acccttacaa atttattaag tttaatacta ttagcgcaag acaaagctac tctaagcaca
    49501 gttgacttca tactaccttt ccgctcagcc tggggaaata gcaatttatc tacaatttca
    49561 ggatgtttct taaccaaaag cggtactgag taccttgcat aaggaatcat tctttcacat
    49621 gccctatcga gggtaggggt gtcgttatcg tagagtaaag catcgctaca aaagaaaaac
    49681 ggaacagagc ttaaacgaag ccgcgcccca aattcttcat cctcacaacc ataatgccgg
    49741 aatgattcat cgaacccttt ctgtgcttca aactcggatt tgctaatcag gaaagcatcg
    49801 cttgcaaagt agcgatactc aattttaaca gttcctttat atctattccc caaatatcgg
    49861 gagtcaaagt agtgtaggta attgctgtcg cgggttaaat cttgccgaca tctgacatta
    49921 gcccttgcta caaaaggctt ctcgcaggac aaaatcattg aatgcaggct atcaagacaa
    49981 ccttgctgga gcaaattgtc tatgtcgaca aaaaataacc a
//
LOCUS       DAHBHC010000029        48878 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHBHC010000029 DAHBHC010000000
VERSION     DAHBHC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.029"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..872
                     /gene="ldtC"
                     /locus_tag="M2A35_002090"
     CDS             <1..872
                     /gene="ldtC"
                     /locus_tag="M2A35_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCB7198390.1"
                     /translation="PLPPAGSRLVGENKFHVVENDGGSLEAIAKKYNVGFLALLQANP
                     GVDPYVPRAGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGKNSVTVYPIGIGQL
                     GGDTLTPTMVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPAGPDNPMGHHAIRL
                     AAYGGVYLLHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGTKVNIINTPIKVSA
                     EPNGARLVEVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAEVMQHVMDVRSGMP
                     VDVRRHQVSPQTL"
     gene            complement(955..1212)
                     /gene="bhsA"
                     /locus_tag="M2A35_002091"
     CDS             complement(955..1212)
                     /gene="bhsA"
                     /locus_tag="M2A35_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCB7198391.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            1453..2085
                     /gene="comR"
                     /locus_tag="M2A35_002092"
     CDS             1453..2085
                     /gene="comR"
                     /locus_tag="M2A35_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCB7198392.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(2147..2686)
                     /locus_tag="M2A35_002093"
     CDS             complement(2147..2686)
                     /locus_tag="M2A35_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCB7198393.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(2896..4200)
                     /gene="ndh"
                     /locus_tag="M2A35_002094"
     CDS             complement(2896..4200)
                     /gene="ndh"
                     /locus_tag="M2A35_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCB7198394.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(4600..5142)
                     /locus_tag="M2A35_002095"
     CDS             complement(4600..5142)
                     /locus_tag="M2A35_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7198395.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(5165..6190)
                     /gene="nagZ"
                     /locus_tag="M2A35_002096"
     CDS             complement(5165..6190)
                     /gene="nagZ"
                     /locus_tag="M2A35_002096"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCB7198396.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFKRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKAQAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(6201..7025)
                     /gene="thiK"
                     /locus_tag="M2A35_002097"
     CDS             complement(6201..7025)
                     /gene="thiK"
                     /locus_tag="M2A35_002097"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCB7198397.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(7006..7647)
                     /gene="lpoB"
                     /locus_tag="M2A35_002098"
     CDS             complement(7006..7647)
                     /gene="lpoB"
                     /locus_tag="M2A35_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCB7198398.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(7661..8038)
                     /locus_tag="M2A35_002099"
     CDS             complement(7661..8038)
                     /locus_tag="M2A35_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCB7198399.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(8041..8400)
                     /gene="hinT"
                     /locus_tag="M2A35_002100"
     CDS             complement(8041..8400)
                     /gene="hinT"
                     /locus_tag="M2A35_002100"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCB7198400.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            8734..10923
                     /gene="fhuE"
                     /locus_tag="M2A35_002101"
     CDS             8734..10923
                     /gene="fhuE"
                     /locus_tag="M2A35_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCB7198401.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGAVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(10983..12416)
                     /gene="ptsG"
                     /locus_tag="M2A35_002102"
     CDS             complement(10983..12416)
                     /gene="ptsG"
                     /locus_tag="M2A35_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCB7198402.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(12711..13508)
                     /locus_tag="M2A35_002103"
     CDS             complement(12711..13508)
                     /locus_tag="M2A35_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCB7198403.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(13519..14523)
                     /gene="holB"
                     /locus_tag="M2A35_002104"
     CDS             complement(13519..14523)
                     /gene="holB"
                     /locus_tag="M2A35_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCB7198404.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(14520..15161)
                     /locus_tag="M2A35_002105"
     CDS             complement(14520..15161)
                     /locus_tag="M2A35_002105"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCB7198405.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(15151..16173)
                     /gene="yceG"
                     /locus_tag="M2A35_002106"
     CDS             complement(15151..16173)
                     /gene="yceG"
                     /locus_tag="M2A35_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCB7198406.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(16176..16985)
                     /gene="pabC"
                     /locus_tag="M2A35_002107"
     CDS             complement(16176..16985)
                     /gene="pabC"
                     /locus_tag="M2A35_002107"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCB7198407.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(17105..18346)
                     /gene="fabF"
                     /locus_tag="M2A35_002108"
     CDS             complement(17105..18346)
                     /gene="fabF"
                     /locus_tag="M2A35_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCB7198408.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(18434..18670)
                     /gene="acpP"
                     /locus_tag="M2A35_002109"
     CDS             complement(18434..18670)
                     /gene="acpP"
                     /locus_tag="M2A35_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCB7198409.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(18881..19615)
                     /gene="fabG"
                     /locus_tag="M2A35_002110"
     CDS             complement(18881..19615)
                     /gene="fabG"
                     /locus_tag="M2A35_002110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCB7198410.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(19628..20557)
                     /gene="fabD"
                     /locus_tag="M2A35_002111"
     CDS             complement(19628..20557)
                     /gene="fabD"
                     /locus_tag="M2A35_002111"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCB7198411.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(20573..21526)
                     /gene="fabH"
                     /locus_tag="M2A35_002112"
     CDS             complement(20573..21526)
                     /gene="fabH"
                     /locus_tag="M2A35_002112"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCB7198412.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(21594..22664)
                     /gene="plsX"
                     /locus_tag="M2A35_002113"
     CDS             complement(21594..22664)
                     /gene="plsX"
                     /locus_tag="M2A35_002113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCB7198413.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPLRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(22745..22918)
                     /gene="rpmF"
                     /locus_tag="M2A35_002114"
     CDS             complement(22745..22918)
                     /gene="rpmF"
                     /locus_tag="M2A35_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCB7198414.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(22970..23491)
                     /gene="yceD"
                     /locus_tag="M2A35_002115"
     CDS             complement(22970..23491)
                     /gene="yceD"
                     /locus_tag="M2A35_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCB7198415.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAEFVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            23690..24274
                     /gene="yceF"
                     /locus_tag="M2A35_002116"
     CDS             23690..24274
                     /gene="yceF"
                     /locus_tag="M2A35_002116"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCB7198416.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDLVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(24386..25345)
                     /gene="rluC"
                     /locus_tag="M2A35_002117"
     CDS             complement(24386..25345)
                     /gene="rluC"
                     /locus_tag="M2A35_002117"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCB7198417.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            25918..29103
                     /gene="rne"
                     /locus_tag="M2A35_002118"
     CDS             25918..29103
                     /gene="rne"
                     /locus_tag="M2A35_002118"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCB7198418.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(29299..30252)
                     /gene="flgL"
                     /locus_tag="M2A35_002119"
     CDS             complement(29299..30252)
                     /gene="flgL"
                     /locus_tag="M2A35_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCB7198419.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(30264..31907)
                     /gene="flgK"
                     /locus_tag="M2A35_002120"
     CDS             complement(30264..31907)
                     /gene="flgK"
                     /locus_tag="M2A35_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCB7198420.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(31973..32914)
                     /gene="flgJ"
                     /locus_tag="M2A35_002121"
     CDS             complement(31973..32914)
                     /gene="flgJ"
                     /locus_tag="M2A35_002121"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCB7198421.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKVGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(32914..34011)
                     /gene="flgI"
                     /locus_tag="M2A35_002122"
     CDS             complement(32914..34011)
                     /gene="flgI"
                     /locus_tag="M2A35_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCB7198422.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(34023..34721)
                     /gene="flgH"
                     /locus_tag="M2A35_002123"
     CDS             complement(34023..34721)
                     /gene="flgH"
                     /locus_tag="M2A35_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCB7198423.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(34774..35556)
                     /gene="flgG"
                     /locus_tag="M2A35_002124"
     CDS             complement(34774..35556)
                     /gene="flgG"
                     /locus_tag="M2A35_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCB7198424.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKVTQL"
     gene            complement(35694..36449)
                     /gene="flgF"
                     /locus_tag="M2A35_002125"
     CDS             complement(35694..36449)
                     /gene="flgF"
                     /locus_tag="M2A35_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCB7198425.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(36469..37674)
                     /gene="flgE"
                     /locus_tag="M2A35_002126"
     CDS             complement(36469..37674)
                     /gene="flgE"
                     /locus_tag="M2A35_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCB7198426.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(37699..38394)
                     /gene="flgD"
                     /locus_tag="M2A35_002127"
     CDS             complement(37699..38394)
                     /gene="flgD"
                     /locus_tag="M2A35_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCB7198427.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     HGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(38406..38810)
                     /gene="flgC"
                     /locus_tag="M2A35_002128"
     CDS             complement(38406..38810)
                     /gene="flgC"
                     /locus_tag="M2A35_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCB7198428.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(38814..39230)
                     /gene="flgB"
                     /locus_tag="M2A35_002129"
     CDS             complement(38814..39230)
                     /gene="flgB"
                     /locus_tag="M2A35_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCB7198429.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            39385..40044
                     /gene="flgA"
                     /locus_tag="M2A35_002130"
     CDS             39385..40044
                     /gene="flgA"
                     /locus_tag="M2A35_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCB7198430.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            40120..40413
                     /gene="flgM"
                     /locus_tag="M2A35_002131"
     CDS             40120..40413
                     /gene="flgM"
                     /locus_tag="M2A35_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCB7198431.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            40418..40834
                     /gene="flgN"
                     /locus_tag="M2A35_002132"
     CDS             40418..40834
                     /gene="flgN"
                     /locus_tag="M2A35_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCB7198432.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(40874..42409)
                     /gene="murJ"
                     /locus_tag="M2A35_002133"
     CDS             complement(40874..42409)
                     /gene="murJ"
                     /locus_tag="M2A35_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCB7198433.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(42519..43442)
                     /locus_tag="M2A35_002134"
     CDS             complement(42519..43442)
                     /locus_tag="M2A35_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCB7198434.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(43444..44091)
                     /locus_tag="M2A35_002135"
     CDS             complement(43444..44091)
                     /locus_tag="M2A35_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCB7198435.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSCAARMYEFSDMAEVESTLEQLANREDGPFVVRLARELGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(44102..44686)
                     /gene="rimJ"
                     /locus_tag="M2A35_002136"
     CDS             complement(44102..44686)
                     /gene="rimJ"
                     /locus_tag="M2A35_002136"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCB7198436.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            44922..46130
                     /gene="mdtH"
                     /locus_tag="M2A35_002137"
     CDS             44922..46130
                     /gene="mdtH"
                     /locus_tag="M2A35_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCB7198437.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            46194..46841
                     /gene="grxB"
                     /locus_tag="M2A35_002138"
     CDS             46194..46841
                     /gene="grxB"
                     /locus_tag="M2A35_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCB7198438.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            46975..47535
                     /locus_tag="M2A35_002139"
     CDS             46975..47535
                     /locus_tag="M2A35_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7198439.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            47641..48687
                     /gene="pyrC"
                     /locus_tag="M2A35_002140"
     CDS             47641..48687
                     /gene="pyrC"
                     /locus_tag="M2A35_002140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCB7198440.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            48761..>48878
                     /locus_tag="M2A35_002141"
     CDS             48761..>48878
                     /locus_tag="M2A35_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCB7198441.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGH"
BASE COUNT        11848 a        13192 c        12158 g        11680 t
ORIGIN      
        1 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
       61 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
      121 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
      181 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
      241 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
      301 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
      361 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
      421 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
      481 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
      541 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
      601 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
      661 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
      721 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
      781 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
      841 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca
      901 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
      961 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
     1021 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
     1081 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
     1141 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
     1201 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
     1261 atcgataact aaatgataga tgtgatctgg atcacataca agatattttt tataacaatc
     1321 attaattaat ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga
     1381 tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac agtcaccagg
     1441 tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca
     1501 aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt
     1561 tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta
     1621 tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat
     1681 cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg
     1741 ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc
     1801 tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg
     1861 ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa
     1921 gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt
     1981 attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actaatgcag
     2041 attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc
     2101 cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat
     2161 tcaaaatcag ttgcccatta ctatctagcg ggatctgcgt acccggatcg cggtccatgc
     2221 ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg
     2281 acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga
     2341 gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga
     2401 catctttacc gcgaccaccg ccaaactgat gccctatcac gccgccagca acagcgccga
     2461 gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt
     2521 tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct
     2581 gagcgtattg cgggccacgt tcaaacacgt tcagactggc cactgccgct acgcccagcg
     2641 cagctgcgac accaatcccg atacccgcca acattgattt attcacggga acacctcctt
     2701 ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga
     2761 aaagcgcggg aatggctgga aatacttaag gattcggagg agtctgaagg aatgcctgat
     2821 gcgacgcggc tgccgatcgc gctgttcctc aagggcggat aaggcgttta tgccacatcc
     2881 gccagtgtac gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc
     2941 caccagcatc attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta
     3001 tagcgagata tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag
     3061 gttacccatc aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc
     3121 tttatactga taatttttca gcggcttacc attcatctgc gccagaatgt tgttcattgc
     3181 gcaagtcgcc atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg
     3241 cgggcatgac gcgcagtcgc caatagcgta aatgtctgga tcgcgggtgg tttgcagcgt
     3301 cggttccacc accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa
     3361 gtctggcgct ttgatcccgg ctgcccacac catcagatca gcctcaatat attcgccatc
     3421 tttagtgtgc aggccgcctt tatcagcact ggtgaccatg gtttgcgtca gcacgcgaac
     3481 gccaagtttc gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa
     3541 aatacgttct cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta
     3601 accgtagctg tgcagttgct tgaccgcgtt gtgcaattca gcggagagtt ctacacccgt
     3661 cgcgccgccg ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcggagta
     3721 tttcaggaag agattcagca tctcctggtg aaagcgacgc gcctggtgcg ggttatcgag
     3781 gaaaatgcag ttctctttga cacctggcgt attgaaatcg ttagaggtgc tacccagcgc
     3841 cattaccagg gtgtcatagg cgattttacg ttccggaacc agcagttcac ctttctcatc
     3901 gcgcagttct gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccag
     3961 ctggaactgg aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc
     4021 atcaagcgag ccagtcgcca cttcgtgcag cagcggtttc cacagatggc tgtggttacg
     4081 atcgaccagc gtaattttgg cttttttctt gcgtcccagc ttatgcccca gctgtgttgc
     4141 catttccagc ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa
     4201 cgtgaccccc ttaaatttat taaccaattg ttaattaatg gttattaaca tagctcttaa
     4261 ttaacaacag gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc
     4321 attggtcata ccaaaattga tgttaatcaa gttttgttgc tgaaaagata ggcgacttta
     4381 ccgggtgaga tcaaaaaaca gagtttattg tttattattt gagcaagtcc ggaagggcga
     4441 tatagggtga agcgtatatc gagggcgttt ggtcgtcctg ataagaggcg catcaggcat
     4501 tgatgctcga atgtcggatg cggcgtgaac gccttatccg acccacagaa tccggagcgc
     4561 atcaggtacc ggcaaggtta cctgatgcga cgagggcatt tacccgaggg ttttgaacgc
     4621 tttaatgcgc tgtaaatgcg gggagatatt cttgaatttg tgcgtctgct cttcgtccca
     4681 gacaatctcg taataatgat gcagctcttc agatgtccgc tggctgttaa gcgcttcatc
     4741 attacgcgac aaaatcacca ggcaacgatc gcgattcttc tcacggaagt tggtcacaca
     4801 cttagtggca atatcggcat actcttccgg gcgatcaatc ttgccttcca tgttctcata
     4861 agggaacaaa ttagggttga agatcacctg gcggatatcg caaagaaaac caatccgttc
     4921 cgcccagtat ccgcccagac caacgccgca aatcagcgga cgctcgtcga cgttcagttg
     4981 caacattttg tccacttctt tgagcagatg ctgcatatca tgtttcggat gccgcgtact
     5041 gtagcttatc aagcgtacat ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt
     5101 acccggactg ttagagtcaa aaccgtgtaa atagataatc atcgcttcct cacataagcc
     5161 agggttagtg acctgctttc tcttcctgcc agcgttcatg taactgattc agacgggtgc
     5221 tgatcgcttt ccagcgagcc gagtccatca gttcctgtcg cgaaaatgaa cctttatgat
     5281 acaaacgtgt aacacgttct gccttgatcg gtgacagatt atctaacacg ctgacggccc
     5341 ctttacgatt attgcagacc aggatcatat cgcaacccgc atccagtgat gcctgcccgc
     5401 gttcggcata actgcccata atcgcggcac cttccatcga taaatcgtca gagaaaatca
     5461 cgccgtcaaa acccagttcc tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg
     5521 ccggacgcgg atcaacatca ctgtagatca catgcgcagg cataatggcg tcgagtttat
     5581 tttcgcggat taaggaactg aagaccgaca tatctttagc gcgaatctcc gcttgtggac
     5641 gtggatcgca cggtgtttct ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt
     5701 gtttcccggt cgttttcatt ccggcttcat gcataccatc aataaaccgg ctggcaattg
     5761 cctgggcttt ttgtggatcg gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc
     5821 cgacatccag cacaggcgca aagctgatat caatatccat agcgatcatt tcgctggcca
     5881 tcagccaacc cgcctcttgc gccagtttgc cgccctcttc cattcccaac agcgcagcaa
     5941 aggattgtgc tgccggtaag cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt
     6001 cctgatctac cgccaccacc agatgattgc gcgaagctgc gcggatctgg cgcaccagtt
     6061 cacgcaactg ggcaggatca tgatagttac gcttaaagag aatcagccct cccaccagcg
     6121 gatgcgcaag tatttcacgc tcttccgcgt ccagttcgta accttcgaca tccaacatta
     6181 ctggacccac actgctctcc ttattgtttt attaatagct gccgccaggt gtcatcggcc
     6241 agcctgataa attgttgatc gccggtttgt cgccagcggt actcaaacca ccctgctttg
     6301 agcatcagca gccagggaaa ccatcgcctg acctgacgcc ataattgcgc cggataaatc
     6361 ttcgcgcgag tggcatagtc attgaccaat tgccggtgct ggtcagtatt ttccacccac
     6421 accgccgcca gttccagcgc gatatcacca tctccggcat actcccagtc gatgagtttt
     6481 aaccctgacg cgctatgcac taaatttccg gcgtggacat ccatatgcaa tggacttaag
     6541 cgtaaaggcc gtggttcccg cgctttgcgc agacgtttta accttcgcag ccaacccact
     6601 gtccgccgcg ccggatcgct ttgctgccag tacagttcca gtaacggcaa cagcgttatt
     6661 cgccagccaa aacgcggttg ttgatgtaga taatacagca agcctgccag ttcgttggta
     6721 tccggcaaat acgtttttac ctcgccgggc agatagtcga ccaccatcca gtcacggaga
     6781 tataaatgcg gcttcggtgc aatacatgcg ggtaattgtg ataaagcccg atactggcgc
     6841 aagaacgcga accgcggcgc atcaggatcg tgcggctgac gtacaacaaa acgctggccc
     6901 tggtgttcaa tgagaaaact cccgccacta agcccactat gaaacgtcgt gacgggatga
     6961 aactgcggga aaaagcgcga cagcaattcg tcgcgcatga tgggattatt gctgcgaaac
     7021 ggcaccttta cctgaccaga taatttcgcc cgtctgcacc agcatcagct gcatttgtag
     7081 ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc acgtaatgag cgccgacatt
     7141 gcgggcaatg cctatggctt tactacgagt acctaaactg tcctgcggcg acaaacctaa
     7201 ctgttgctta gccatcgaca gctgctgggc ggaaaccagg gtaaatttcc cgttattagc
     7261 cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt
     7321 gttaacgcta tcaaccagca ggacgctacc cgcagtcacc ccgtcagccc caagcatctt
     7381 actgaccatc ggctgcattg cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg
     7441 tgccgtttga tcttcgtgct caattgggcc tggctgctgc gggatcgtcg gcaccgaggg
     7501 cactgtgggg acaggctgtt gtggctcggc tggttgttcc ggcgctggtt tcacttcttc
     7561 taccggtgca ggttcacgtt gccccacaca cccggcgaga aacatcgcca gcgcggtaat
     7621 caaggcgtag cgactcattt ttgtcatcaa gattcacccc ttacaaatat agataaagtc
     7681 tgactttgtg cgcccccaga aaattggcgc tgccgtacag cgttaccgcc gaatgtgcgg
     7741 gaatgctaac gctgcgtggc ctttccagag gatgcatctc cagccctctg gcgtcatacc
     7801 agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc gttataaagc gttgaggatg
     7861 ctgaaggttg aatatcagac gtcgaaagga cgggcttttc tgcactgatg cccgcagcca
     7921 ataaagatga ctccatcacc agcgattgct catcattcac cggaatttcc ggatgtgaac
     7981 gacagcccac cagcagtaac aacgccagag acaccagccc aaagcatcct tttctcatcg
     8041 ttacagacct ttatgcgcca gcattggtcc cagcggacgg ccacccaaca agtgcatatg
     8101 gatgtggtaa acctcttgtc cgccatggcg gttggtattc atgatcagac gatagccatc
     8161 ttcggcaata ccttcttgct cagcaatttt tgccgctacg gtgatcatgc gtcccagcgc
     8221 ctgctcatgc tcagctgaga cgtcgttcac agtcggaatg aggatattcg gaatgatcag
     8281 aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat catcctggta
     8341 gacgatatcg gaggggatct cacgacgaat aattttgctg aatatagttt cttctgccac
     8401 gacattttcc tttttcaaat agccatgcgt tgcgccatgc tacgccgcgc cacactgcga
     8461 gtatagagta tggtcgagtt aaacctgctc tttcaacctt aactcacgat ttcttaagca
     8521 aaaaatcgtt ttgctgccgt atatatcgcc attattccca tttctgcctg cgaatgcgct
     8581 gaaaagacat tctctgtttg gtttacacga caaatatgaa tgcgtatatt tctcatttgc
     8641 atttacaaac aaaattattc gcacactaaa tataatcgct catcaccctc ctccggcatg
     8701 agcctgacga caacataaac caagggattt caaatgcttt caacacaatt taacagggat
     8761 aatcaatatc aagccatcac caaaccgtca ctacttgccg gttgcatagc actggcacta
     8821 ttaccttctg ccgcttttgc tgcaccagcc actgaagaaa cggtgattgt tgagggttca
     8881 gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc
     8941 aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttag ccagcagcgg
     9001 atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct ggggatcagc
     9061 aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca gatcgataac
     9121 tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg cgacgcactt
     9181 tctgatatgg cactctttga acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc
     9241 gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac cagtcgtgaa
     9301 tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta tgtggcggat
     9361 ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg cggctaccag
     9421 aataacgact catggctgga ccgctacaac agtgaaaaga ccttcttctc cggcattgtc
     9481 gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca gcgcattgat
     9541 gttaatagcc caacctgggg cggtttaccg cgctggaata ctgatggcag cagcaacagt
     9601 tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca acgataaaga gatcaacaag
     9661 gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacgct gaatgccacc
     9721 cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg
     9781 gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta tgtcggcggc
     9841 accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt
     9901 tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag caaacaaaac
     9961 aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag tttctacaac
    10021 tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcgca ggacgatacc
    10081 acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg
    10141 atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag catggagaaa
    10201 aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg gtcgacctac
    10261 gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg caaatatctg
    10321 gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat gaatagccgt
    10381 ctgaccacca cgttagccat cttccgtatt gagcaggata atgtcgctca gtccaccggt
    10441 acacctatcc ccggcagcaa cggcgaaacc gcctataaag cagtggatgg ggcagtcagt
    10501 aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct gacatttggc
    10561 gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc
    10621 accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt gacagtcggc
    10681 ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta tggcaccttc
    10741 cgcgccgagc aaggtagcta cgcgctggtg gatctcttca cccgctacca ggtgacgaaa
    10801 aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga taccaacgtg
    10861 gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc
    10921 tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttaca
    10981 gattagtggt tacggatgta ctcatccatc tcggttttca ggttgtcgga tttagtaccg
    11041 aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg
    11101 ccagcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg
    11161 tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg
    11221 ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc
    11281 agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc gataccgacg
    11341 atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg
    11401 tgcgagaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct
    11461 gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga gaactcgatc
    11521 ggctcggtga taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact
    11581 ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg
    11641 tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa
    11701 cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc
    11761 tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa
    11821 ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg
    11881 attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc
    11941 aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc
    12001 ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat aatccctccg
    12061 agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa atgcagtacc
    12121 agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac tgcggccagc
    12181 gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc
    12241 atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc
    12301 cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag tacggatacc
    12361 ggcagcatca gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt
    12421 gagagtgctc ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagag
    12481 cctcgccgtg tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca
    12541 gtgccaattt gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat
    12601 tttgacaaaa catatgtcac aatatttcaa acgtggttat tcaccgccca attttcggcg
    12661 gtgaacttta ctcgttttag ccattaatta cgagctttaa aaaaactcat tcaacggatg
    12721 gattgaaggc gggaagcgtc gatgtgaaac agacgggcga agttatcggt ggttacctgc
    12781 gccagttctt caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc
    12841 atcgcaggtt gattctcttt tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt
    12901 tccaccagta atcgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta
    12961 cggaaggtca caatgccgga aaaggagatg taaaatccga gatccagtaa tttacccgcc
    13021 gtttctctgt cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg
    13081 cgcagaattg ccagcgtatc ggcacgggcg tcacgggtat ggacgataac cggcttgttc
    13141 agttcacgac caatctggat atgatggatg aaggactctt gctgacgtac tttagtttcc
    13201 ggtgtgtaat aataatccag cccggtttca cccagcgcta caacaccctc ttctgccgcc
    13261 agacggcgta aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa
    13321 gaaaatacga cgttgtcacg ttcgcctacc agatcccgca tatgtaaata acccggtaat
    13381 gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca
    13441 tccacgtcct tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact
    13501 aaaaacatga tgtctctctt aaagatgagg aaccggtagc acaacgcccg gttgcaggta
    13561 atgctcaata cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtaacaga
    13621 cattaactgt tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc gcgagggaga
    13681 aagatggttt gccagttcgg cgaccaggcc cggcacatca acattggtca cctgcgcagc
    13741 accatgatgg cgttttagcg catccatcag caacgttgcc agccagtgta aacgcgccgg
    13801 agcttgttca tgattaaggg ccgctagcag cgaataccag tcgcccgatg gcacgctata
    13861 tgccaacgcc tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag
    13921 tgccgcgcca ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat cctgtgacat
    13981 tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg caaggtaatg
    14041 taaccgacaa cgactacgta atgttgccag taaacgttca ggctcgcggg tagccaggaa
    14101 aaaccaggtt tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc
    14161 ggttagtaag gcagcatcgg ttacccagac aactttcgca ccacctaagc gtgcgtgctc
    14221 attcagcttt tcggtgacct cacgtaccgc atcaatgccc aacgcatttt tccctttttc
    14281 gggagccagg gtgtagtaat cgggatgcgt gccagcctgc atcaactgac atccacgaca
    14341 gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt aaataacggc tcagggcgta
    14401 gattaaagca tcatcgccca tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct
    14461 tccggcctga tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct
    14521 catgcgtcca actccttaac ccagtgggtc acggtagtgc ggattgcatc catcacggcc
    14581 tccagcggct gggtggcatc aatggtatga atacttttat cttgtgcagc cagttccaga
    14641 tagcgggcgc gggtgcgatt aaagaaatcg aaagactctt gctcaatacg atccagctcg
    14701 ccgcgcgcac gcgcgcgttt taagccaact tccggggtaa catcgagata gagcgttaag
    14761 tcggggcgaa aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata
    14821 ccacgtcctc cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc aatcacccag
    14881 gtgccgttag ccagagctgg tttgatgacc gtttctacca gttgaacgcg cgcggcataa
    14941 aacatcagaa cttcggcttt atcggtaatg acttcatcgc ctaccgattt gatatccagc
    15001 accaggcttc ttaacttttc ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg
    15061 tcgcggatac ccagttgctc aagcgtctca accaccacat tacgcgcggt agttttgcct
    15121 gcgccttcca gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt
    15181 cagataatcc tgcacagact tgttatggct ggcaagattg gtattaaacg tgtgaccacc
    15241 tttaccatcg gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag
    15301 cgaatccgcc cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt
    15361 atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat
    15421 cccgtaaatc acggttgggt cggtctgcag gcgcatacca atgcgtaaac ggttgataaa
    15481 taccgaggca acctgatcgc gttcactggc aacggcggtt tctttttcga taattgatgc
    15541 catcgtcacc agctgatttt tatctttata aggcagaccg tccgcacgcc cttcccaggc
    15601 gctatcgacc gctttcacca ttttcttgtg cgctcgcttg agtaacgcga catcggtggt
    15661 attggcggta tacatccagg tgtctggcca gaaccaacct tcaatccact ccgggttttc
    15721 cagttcaagt gcctgcgcta cggtggcgta cttatcgtca ctcagcgtgt gcttgatata
    15781 cggagcctca cgcaattgct tgaggtagtc gctcagacgc atcccttcta ccagtcgcag
    15841 cgggaactgt gcttctttac cgctttccaa caatttcagc atctcgcgca cggtcatctg
    15901 cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcag
    15961 ccattgaaaa acccgtgggc gattgatgat cttatcagca taaagctgtt caccgagcgc
    16021 cagacgtccg gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct
    16081 gtcggcaaga tggcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag
    16141 caataacaag attatcaata acactttttt catgactaat tcgggcgctc acaaagtggg
    16201 gctaaatact catataacgt tgctgacgaa aaggagacat cgccacaggc acgtacgggc
    16261 atcactggca ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcttccaga
    16321 gaagcttgca cttcgacaag ctgataagag gattgtgcca gcaaacggat acagaattgt
    16381 cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg tataaactac gttgcccttc
    16441 cgccagaaca aattagccgc acagcattcc gtaacccacc cttcgctgtc aaggaccagc
    16501 gcctcatcag cgtttgtctg ctcaagatga gagcgaatca atacttgctc aagacgattg
    16561 agatgtttaa tacctgcaag atgaggattg cgccccagtc gcaccgggct tagcgccaac
    16621 gtcatcccct cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc
    16681 cgcgttgccg gtccactgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc
    16741 acgactttca gtacaccatt ttgctgttct gctgccagcg ttttcatctc ttgttcaagc
    16801 tgtggccaga agtcacagga aatcatcaac cgctgacaag catcctgtag tcgctggata
    16861 tgcgccgata acaaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca
    16921 ccaaactgcg ttgcccgatc gcttactgcc agcgattcct gcttataacc gttaattaag
    16981 aacataccgg ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag
    17041 tcggaataaa agctaagaaa aaaggcccgc aagcggacct tttataaggg tggaaaatga
    17101 caacttagat ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac
    17161 acagcgtgta ttccattccg ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc
    17221 aaccttcatc cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca
    17281 ggatggagta gatagattct actgcacccg ccgcacctaa cagatgaccg gtcatagatt
    17341 tcgtggagct taccaacacc cggcttgcag cttcaccgaa gatggttttc accgcctgcg
    17401 cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac
    17461 tcgcttcaat gcctgcatca cgcagagcat ttgccattgc cagagctgcg cctgcgccat
    17521 tttctggcgg tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag
    17581 cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg
    17641 caccatcgcc cagtacgaag ccatcacgct ctttatccca cgggcggctc gccgcttgcg
    17701 ggttatcatt gcgggtagat aatgcacgtg ccgcgccaaa accaccaaca cccagcggcg
    17761 tactggcttt ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac
    17821 gcgcagcatg accaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg
    17881 ggccacgcag gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg
    17941 gaacgaagaa tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt
    18001 cgatcagtcc gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct
    18061 cttccgttat ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt
    18121 gaatgaaggc atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat
    18181 cctttactaa gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc
    18241 tgatgccact ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag
    18301 gagacaacat gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccagg
    18361 gagggaaaaa atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc
    18421 tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg
    18481 tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta
    18541 ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg
    18601 taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga
    18661 tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt
    18721 ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat
    18781 tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac tcataaccac
    18841 gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc
    18901 cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa
    18961 ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac
    19021 ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa
    19081 caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga
    19141 tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca
    19201 cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt
    19261 ttgacagacg gaaaacagat gaaaggttgg tttcgataat atcgttccac tcttcatctt
    19321 tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt
    19381 caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat
    19441 tcaacatcag acctttgccg ttagcaccta aataatcact gatcgcctga gcgccatttt
    19501 cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc
    19561 ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt
    19621 tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg
    19681 tcagggtgtc gacaatgcgt ttcgtcaggc cagttagcac tttgcccggg ccgacttcat
    19741 agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt
    19801 tatacaactg acgtaccagt gcgtcacgaa tggcatcacc attggtttcg catttcacat
    19861 caacgttatt cacaacagga actgttggtg cgttaaaggt gattttcgct aattctactg
    19921 ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg
    19981 gcagcgcgcg ttttgcgccc gcagctttac aggcagcgcc agcacgctca accgcttctt
    20041 tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac
    20101 cttctgcagc ttcttcacac gctttcgcaa tagacgcatc atccagaccg atgattgccg
    20161 ccatagcgcc agtgccttcc ggtacggctt cttgcatgaa cttaccgcgc atctcaacca
    20221 gacgcaccgc atcagcgaaa tcaatcacgc cagcgcaaac cagcgcggag tattccccca
    20281 ggctgtgacc ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcgataca
    20341 gcgcaacaga tgcagtcaac agcgctggct gagtttgcca ggttttattc agttcttcag
    20401 ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa
    20461 acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag
    20521 aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac
    20581 gaaccagcgc ggagccccag gtgaatccac cgccaaaggc ttcaagcaga accaactgcc
    20641 ccggcttaat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag
    20701 aggtattacc gtggcgatcc agcgtcacca cgacattgtc catagacata ccgagttttt
    20761 tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag
    20821 aacggtcaag attatttgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg
    20881 caaccttgaa gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacac
    20941 ggtcagcatt aggcagcgtc agcaactcac catagctacc gtcggcatgc agatgggtgg
    21001 agatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa
    21061 taataatagt cccacgatcg gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga
    21121 ccagagcata cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg
    21181 tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccagcatgc
    21241 tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga
    21301 tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa
    21361 agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac
    21421 gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca
    21481 cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac
    21541 ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc
    21601 agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg
    21661 cgagcggcaa ttcgcagagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg
    21721 accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct
    21781 aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc
    21841 ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga
    21901 gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa cgtgacattt
    21961 cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca
    22021 agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata
    22081 ctgtcgagac cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga
    22141 atttccacca cctcttcagc caggactgag cccataatgg caaattgcac caacattgtg
    22201 ctgtcacaat cgacgttggc ccctaaatcg agaaccaccg ttttgccctt ttgctgatgc
    22261 ggcaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt
    22321 gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct
    22381 ttcaccaact ccagggccac gcgcattgaa ctcccacgac tggcgcggat agcttgcgaa
    22441 ggccggacat cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt
    22501 tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga
    22561 gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca
    22621 aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc
    22681 ggtttggttt ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc
    22741 gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac
    22801 gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt
    22861 catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg
    22921 accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct
    22981 ttaagctggc taatacggca aatgggtttg gcttttgcgc ttcttcaggc agttcaccaa
    23041 agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca
    23101 aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa
    23161 cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac
    23221 taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga
    23281 gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga
    23341 acgacatgga gcattccaca tcactgtcca cactgactac gaattcggcg acgcgctcaa
    23401 cctgatcagg ggtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat
    23461 cgagagtcag gggtaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca
    23521 tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg
    23581 ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc
    23641 atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact
    23701 tattttagcc tccacctcgc catggcgtcg cgctcttctg gaaaaactgc aaatctcttt
    23761 tgaatgtgca gcaccagagg tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt
    23821 ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt
    23881 aattattggt tcagacctgg tatgtgttct tgatggcgaa atcaccggta aaccgttaac
    23941 ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccttctatac
    24001 tggactggcg ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg agccttttga
    24061 cgtccatttc cgtcacctga gcgaggcgga gattgataat tacgtgcgta aggagcatcc
    24121 cctgcactgc gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt
    24181 agagggacgt gaccctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt
    24241 acgtcgggaa gggaaaaacc cgctgatggg atagttgttt gcgccggatg cggttacgtc
    24301 gcatccggca atcggtgcag gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc
    24361 ccattatcag gcttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct
    24421 tcaaaccttc atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa
    24481 acttcaacgc tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct tcagtgagct
    24541 gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact
    24601 gtgtatgcac acggatctga tgagtacgtc cggttaccgg gctacaacgc accagggtgg
    24661 caaatgcata gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactga
    24721 cacgcacgat acgttcgccg ctttgcagaa tatttttcaa taacggcgct tgaacgctct
    24781 tcacatgcga ctgccactga ccgcgcacca gcgccaggta atctttttgc attccttttt
    24841 cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac
    24901 ctgaagtgtc ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg
    24961 cccgcaaacc ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg
    25021 aaggtttatt cagcaccagg atatgatcat cttcatataa gatgacgtcc gccagcgccg
    25081 ccaccttttg cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg
    25141 ggatacgcac ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca
    25201 cccgcacttc acctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt
    25261 gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc agcggtgata gcaacaattt
    25321 ttacggatgg agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc
    25381 cactcatttt tctatgctta tatttagttt tacaccttat tacttcacta cgtgatcact
    25441 ttattgatgg ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt
    25501 gataaatggt aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt
    25561 ccgtgtccat ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat
    25621 catccacgca gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt
    25681 tgcagtcctt accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg
    25741 ataattgcgc tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc
    25801 tgacgataac cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga
    25861 aaggcgttac tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg
    25921 aaaagaatgt taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg
    25981 cagcgtctgt atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc
    26041 tacaaaggta aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc
    26101 gctgaacgtc acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac
    26161 tacagtgctc atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt
    26221 gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt
    26281 ctggcgggta gctatctggt tctgatgccg aacaacccgc gcgcgggtgg catttctcgc
    26341 cgtatcgaag gcgacgaccg taccgaatta aaagaagcac tggcgagcct tgaactgccg
    26401 gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca aatctgctga ggcgctgcaa
    26461 tgggatttaa gcttccgtct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc
    26521 ccggctccgt tcctgattca tcaggagagc aacgtaatcg ttcgcgcatt ccgcgattac
    26581 ttacgtcagg acatcggcga aatccttatc gataacccga aagtgctcga actggcacgt
    26641 cagcatatcg ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc
    26701 gagatcccgc tgttcagcca ctaccagatc gagtcgcaga tcgaatccgc cttccagcgt
    26761 gaagtccgtc tgccgtccgg tggttccatt gttatcgaca gcaccgaagc gttaaccgcc
    26821 atcgacatca actccgcacg cgcgacccgc ggcggcgata tcgaagaaac tgcgtttaac
    26881 accaacctcg aagctgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc
    26941 ctgattgtta tcgacttcat cgacatgacg cctgtacgcc accagcgtgc ggtagaaaac
    27001 cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc
    27061 tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat cactgggtga atccagtcat
    27121 cacgtttgtc cgcgttgttc tggtactggc accgtgcgtg acaacgaatc gctgtcgctc
    27181 tctattctgc gtctgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc
    27241 attgttcctg tgccaatcgc ttcttacctg ctgaatgaaa aacgttctgc ggtaaatgcc
    27301 attgaaactc gtcaggacgg tgtgcgctgt gtaattgtgc caaacgatca gatggaaacc
    27361 ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa ccccaacctt aagctacatg
    27421 ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt
    27481 aagcgtccgg aacaacctgc gctggcaacc tttgccatgc cggatgtgcc gccagcgcca
    27541 acgccagctg aacctgccgc gcctgttgta gccccagcac cgaaagctgc accggcaaca
    27601 ccagcaactc ctgcacaacc tgggctgttg agccgcttct tcggcgcact gaaagcgctg
    27661 ttcagcggta gtgaagaaac caaaccgacc gagcaaccag caccgaaagc agaagcgaaa
    27721 ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca accgccgtga ccgtaatgag
    27781 cgccgcgaca cccgtagtga acgtactgaa ggcagcgata atcgcgaaga aaaccgtcgt
    27841 aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg agagccgtca gcaagctgag
    27901 gtaacggaaa aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc
    27961 cgccgtaacg atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag
    28021 caatctgttc aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa
    28081 cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc
    28141 gcaccggtgg ttgaagaaac tgccgctgcc gaaccaattg ttcaggaagc tccagctcca
    28201 cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa
    28261 gaagagaaca atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg
    28321 cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca
    28381 acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa
    28441 gtctggatcc gctatccaat tgtacgtccg caagatgtac aggttgaaga gcagcgcgaa
    28501 caggaagaag tacatgtgca gccgatggtg actgaggtcc ctgtcgccgc cgctatcgaa
    28561 ccggttgtta gcgcgccagt tgttgaagaa gtggccggtg tcgtagaagc ccccgttcag
    28621 gttgccgaac cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat cgctgccgcg
    28681 gtaactgaac agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagttgca
    28741 gaacaagcag aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgtc
    28801 gaaactgctg aggttgtagt tgctgaacct gaagttgttg ctcaacctgc cgcgccagta
    28861 gtcgctgaag tcgcagcaga agttgaaacg gtagctgcgg tcgaacctga ggtcaccgtt
    28921 gagcataacc acgctaccgc gccaatgacg cgcgctccag caccggaata tgttccggag
    28981 gcaccgcgtc acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca
    29041 ggtggtcata cggcaacaca tcatgcctct gccgctcctg cgcgtccgca acctgttgag
    29101 taataattag ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatctt
    29161 tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat gcaaaatagg
    29221 caaaaaaatg cccgccccag cgatcccaga gcaggcagac aaaacatacc cagtttcatg
    29281 atatgtttta aagcgaaatt atttgttgag ctggaagagc gacaatccct gcatatcggt
    29341 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    29401 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    29461 acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacagt
    29521 cagcacattg ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc
    29581 ggtttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcactatc
    29641 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttgct
    29701 ggtaatactg tcgaaaattt tgtcacccgt gtgccctatc accatcgaac gcgaagcatc
    29761 gacctgttgt ttaatacttt ctgctccacc gacgtatttc cctttctctt cgctaaacgg
    29821 cgcagtctct gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc
    29881 cagattcagc aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc
    29941 gtcactcaag gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    30001 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgtgc
    30061 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc
    30121 aatgggatcg tctgaagggt taacgactcg cttacccgtc gacatctgct cgccgtactt
    30181 catccattct gcctgagaat tggtgatgcc gcgcatgttt tgctggtaca tcatctgggt
    30241 actgaaacgc attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    30301 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    30361 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    30421 acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    30481 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt
    30541 tgcagatcca gcagggcctg accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc
    30601 gccatcgcga ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt
    30661 actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga
    30721 ccatcaaatg ccactttacc gttggcatct ggtgtcgccg taaaagtggt attgctggca
    30781 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc
    30841 acggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc
    30901 agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat
    30961 ccggctttgt gttggctgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    31021 ttacgcgtct ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    31081 ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc
    31141 gtacgagaag ggtcggcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga
    31201 accagtgagt aaccattggc catcgtgatg ttataagtac cgccatcctg aacgctgact
    31261 tctacgccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    31321 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    31381 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    31441 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    31501 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    31561 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    31621 tcgattttcg acatctgctc atagcgggca gtcaggccgc tactttgcgt ctgcgcagca
    31681 cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca
    31741 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    31801 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    31861 tgggccgcgt tcagtccgct catggcgtta ttaatcaagc tggacatgga ggttccttat
    31921 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    31981 gattatcaat gttcatactg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    32041 gaatcatgtt ggtgagtttg cgggcatagt gaggatcggt ggcatagccc gcgtcctgta
    32101 gggcctgcgc cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac
    32161 gcgttaacag cccaacgtaa tccgacaagg cttccagata cgagctgtag acgcgaaact
    32221 tcgcttttac tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtaactg
    32281 gccctttcca gttgccagag gctttgacac caaacaggtt atagctcggc tcgccgtttt
    32341 cgcggcggat ttgccgttgc ccccaaccag attccagtgc cgcctgagcg aggatcaaat
    32401 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    32461 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    32521 gctgcgaaag cgcctgattt tgataacgca ccacagtttc gagcgggaat ttcatcggtg
    32581 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    32641 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac
    32701 tggtatacag acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    32761 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    32821 ttgccgccgg atcttcgccc actttcgcct ttagttcgtt gagtgattgc gcatcccagg
    32881 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    32941 acatcccgca ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag
    33001 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    33061 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    33121 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    33181 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    33241 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    33301 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    33361 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    33421 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    33481 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    33541 cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    33601 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    33661 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    33721 tccaaacgga ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    33781 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    33841 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    33901 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    33961 agccgccgtc gtgaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    34021 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    34081 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    34141 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    34201 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    34261 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    34321 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    34381 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    34441 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    34501 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    34561 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    34621 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    34681 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    34741 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcactt tttgcagcat
    34801 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    34861 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    34921 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca ccagaggatt gcgtttcggt
    34981 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    35041 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    35101 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    35161 accagccgtc accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc
    35221 tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt
    35281 gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg
    35341 tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc
    35401 cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc
    35461 gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc
    35521 aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg
    35581 acaagcagag cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa
    35641 aggggcgagg gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc
    35701 atcgacagca gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc
    35761 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    35821 ttactgcctt ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtatcggc
    35881 ccacgcgtgg cctggctttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgttgcact
    35941 tcgctgcccg tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga
    36001 tcgcccggat tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc
    36061 ggcacagcaa ttggcccagc ctcgcctatc accggatgcc cctgaattgt cagttgcccg
    36121 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggactgc
    36181 acggccagcc agccatcctg ctgcaatgca acgtccagcg ggcgcgaggt gtaatccatc
    36241 ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt
    36301 gaaagccctt ccaccggcac tgcacgcaag gcgtttaact gcgcgcgaaa accgggcgta
    36361 gaggcattgg ccagattact ggcggttacc gcctgttgat tcagcgtctg gctggctgct
    36421 cccatcgcgg tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    36481 aaccagcgtg ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt
    36541 acgctgggca acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccaa
    36601 cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc
    36661 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    36721 aaagttcgcc agtacaatct gccccagcag ttgtgtttgt tcgttggaat agttgccgac
    36781 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccattctg
    36841 ggtggttgcc acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag
    36901 gctaaacgtg gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt
    36961 tgaggtcaga acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt
    37021 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    37081 gacgctcatg tcatgagcat taccctgact gtcgaaaaca gtcaccgaac cttttttgtt
    37141 atagctatcc gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cactggaatt
    37201 caggttgatc tgcatcgatg ccgtggtggt agttttcgct gccatcaggg tattcgggat
    37261 cgaaatattg gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    37321 acccgtcagc tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg
    37381 tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg
    37441 gctgatagca acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg
    37501 agtgataccg gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    37561 agaggccgtg cctgatttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac
    37621 atcgaggttg gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    37681 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    37741 agtgccgaga tccagcgtat taccgctgtt gccgcggatc acaccctgca ccagagcaaa
    37801 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacattgta
    37861 agaaccgttc ggcgcagtgc tgccgtcagt caacgtaccg tcccaggtga aactgtgaac
    37921 tccggcggtc agttcaccaa tatcaatggt gcgcacaacc gcaccatttt tatcggtgat
    37981 ggtggccgta actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    38041 cacagctcct tcttcactgc cggttcccgc aagaacagtg gtgccgtgga tcatcacgcc
    38101 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    38161 tccgagtgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga
    38221 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccag
    38281 caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactgct
    38341 ggtggtactg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt
    38401 ctcctttatt gaccgagcgt aagggttttc agcatcatgc ttttcaccgt gttgagcact
    38461 tcaacgttgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca
    38521 tcaacgttcg gcatttttac gtagcccttt gcatctgcca gcggattacc cggttcataa
    38581 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    38641 gcacctggtg cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca
    38701 ggaccggtca cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac
    38761 tgggcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    38821 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    38881 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta
    38941 ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    39001 gcctgcgccg ggatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc
    39061 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    39121 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    39181 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    39241 caggtatcaa aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    39301 aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg
    39361 ttgtagaaat ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg
    39421 atactgttca gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt
    39481 agcgcccaac tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcccccaat
    39541 ctactaccgc catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc
    39601 aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag
    39661 gccacaggaa attatgtggt tgccgcgatg cccattgtgc ggggaggaaa gctggaagct
    39721 ggcaatgtca aactaaaacg cggacggctg gataccctgc caccgcgtac ggtgctggat
    39781 atcaatcaac ttgttgatgc cattagcctg cgcgatctat cacccgatca acctatccag
    39841 ttaacccagt ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc
    39901 agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca
    39961 cagaatgcgc gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat
    40021 gggaatattc ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa
    40081 gcaacacatg ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg
    40141 cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac
    40201 agccgggcgg caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa
    40261 gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa
    40321 ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc
    40381 aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct
    40441 cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca
    40501 tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc
    40561 actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa
    40621 tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca
    40681 aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct
    40741 ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac
    40801 aacccatcgc ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg
    40861 ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg
    40921 ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc
    40981 gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc
    41041 cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg
    41101 gggtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg
    41161 ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca
    41221 tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc
    41281 gggaataaaa gccaggagcc aaaactttca ctacaatcag gccaatcaaa cccaccgagt
    41341 aggcaattaa cgcccgctgg gtcatcagcg catcaaaggc ggtaaattta ccgtactgga
    41401 acagcgaaac ggtcaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca
    41461 acaggaaaca aagacgcagc ccccagtcca tcaaacggtt gtattcatca tgattgccac
    41521 tggcaaaact cttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac
    41581 cagacggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa
    41641 gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga
    41701 tcgccggtcc catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg
    41761 gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc
    41821 cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa
    41881 acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg agcaaacgcc ggaatcgaga
    41941 agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat
    42001 agggaaaggt aatctttagt agctggctgg tcagggcaaa tttgtcggca gtgtcagcga
    42061 agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa
    42121 ccgccagcgc gagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt
    42181 caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt
    42241 cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg
    42301 ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg
    42361 aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa
    42421 tccagaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct
    42481 ggattatggt gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc
    42541 cagatcttgt caacgatacg ttgcgccagc acggcctgtt cgccggcggt ttgcggaact
    42601 gtctgatttt gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca
    42661 agagtgctct gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc
    42721 cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca
    42781 cgctgacttc cggcacggcg atgcatacag gtggtgatct gcaaaggacc ggccgaaaaa
    42841 tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga
    42901 gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc
    42961 aacgtgaaat aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc
    43021 gaagctgcgg tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta
    43081 aaaccgacca tcaacgtcag ttttttacgc gccgccagtt ccaccagccg ttcagcatcg
    43141 cgcagatttt ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta
    43201 ctgaccacgt caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg
    43261 gcaaggctgg ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct
    43321 ttcgcgcgcg taggcgacca ggctccttgt aacgtccagt cagacgctgc cgccagcacc
    43381 ggtaaccacg ctttttgcgc aatgccacct aatcccacta cgccgatacg taattttttc
    43441 actttaatct cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc
    43501 gatttccagg gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt
    43561 caccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt
    43621 acccagttcg cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc
    43681 cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcagctgcg
    43741 cagttcacct ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc
    43801 tgcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac
    43861 ccgattacca aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg
    43921 ttcctgcact tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca
    43981 ggccgtgact acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc
    44041 cagcaagcaa ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc
    44101 cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt
    44161 gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca
    44221 gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt
    44281 gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct
    44341 gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat
    44401 tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga
    44461 aagctgaacc ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg
    44521 gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg
    44581 cgtaataatc cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt
    44641 ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc
    44701 ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg
    44761 aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa
    44821 gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg
    44881 cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg
    44941 cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct
    45001 ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca
    45061 tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg
    45121 gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg
    45181 ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc
    45241 tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac
    45301 taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg
    45361 gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct
    45421 gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg
    45481 catggaaact ctccaccgta cacacgcccg ttcgcgaagg catgacccgc gtgatgcgtg
    45541 acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag
    45601 tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcaccctct gccgttaaat
    45661 ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga
    45721 gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa
    45781 gcatgatgcc ggtgggcatg gtcagcggcc tgcaacaact tttcaccctg atttgtctgt
    45841 tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg
    45901 acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg
    45961 ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag
    46021 agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc
    46081 agtttagtca gaaacgcgcc gcgcgtcgtt tgcttgaacg cgacgcctga tttgatactc
    46141 ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc
    46201 tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa
    46261 atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg
    46321 tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca
    46381 tggacatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt
    46441 cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgcaaacaaa ctgctgttgc
    46501 cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg
    46561 tcgacaagaa agaggccagc gcgggtaatt tagccgacct gctggcccac tctgacggtc
    46621 tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg
    46681 ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga
    46741 cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga
    46801 aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag
    46861 cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg
    46921 acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac
    46981 aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc
    47041 acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc
    47101 tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc
    47161 agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac
    47221 atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc
    47281 gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg
    47341 gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca
    47401 ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc
    47461 gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt
    47521 ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa
    47581 cgtttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta
    47641 atgactgcac catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc
    47701 cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct
    47761 attgtaatgc ccaatctggc tccgcccgtg accactgttg aggctgccgt ggcgtatcgc
    47821 cagcgtattc ttgatgccgt acctgccggg cacaatttca ccccattgat gacctgttat
    47881 ttaacagatt cgctggatcc taatgagctg gagcgcggat ttaacgaagg cgtgttcacc
    47941 gctgcaaaac tttatccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt
    48001 gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat
    48061 ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc
    48121 gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc
    48181 accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact
    48241 ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg
    48301 tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc
    48361 agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa
    48421 gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct
    48481 accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc
    48541 ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa
    48601 caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa
    48661 acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt
    48721 tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat
    48781 agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg
    48841 ccgtattcag tatgcgtttc ctgataatga aggccacg
//
LOCUS       DAHBHC010000030        48755 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHBHC010000030 DAHBHC010000000
VERSION     DAHBHC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.030"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..248)
                     /locus_tag="M2A35_002142"
     CDS             complement(<1..248)
                     /locus_tag="M2A35_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCB7198442.1"
                     /translation="MKSDVSNSRLMISNPTHIAIGIYFKPHLSPIPLISVRVTNEVAL
                     GVRKYAKEIGIPIITDKKLARKNYATHRRYDYVSFENI"
     gene            complement(245..976)
                     /locus_tag="M2A35_002143"
     CDS             complement(245..976)
                     /locus_tag="M2A35_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCB7198443.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGGGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIIDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERIRKFFLSN"
     gene            complement(985..1752)
                     /gene="sctT"
                     /locus_tag="M2A35_002144"
     CDS             complement(985..1752)
                     /gene="sctT"
                     /locus_tag="M2A35_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HCB7198444.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1754..2014)
                     /locus_tag="M2A35_002145"
     CDS             complement(1754..2014)
                     /locus_tag="M2A35_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HCB7198445.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIEVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2024..2689)
                     /locus_tag="M2A35_002146"
     CDS             complement(2024..2689)
                     /locus_tag="M2A35_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HCB7198446.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2679..3626)
                     /locus_tag="M2A35_002147"
                     /pseudo
     CDS             complement(2679..3626)
                     /locus_tag="M2A35_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(3626..3745)
                     /locus_tag="M2A35_002148"
                     /pseudo
     CDS             complement(3626..3745)
                     /locus_tag="M2A35_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(3758..5368)
                     /locus_tag="M2A35_002149"
     CDS             complement(3758..5368)
                     /locus_tag="M2A35_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198447.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVMHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            complement(5602..5675)
                     /locus_tag="M2A35_002150"
     tRNA            complement(5602..5675)
                     /locus_tag="M2A35_002150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5641..5643),aa:Gly,seq:ccc)
     gene            complement(5754..6509)
                     /locus_tag="M2A35_002151"
     CDS             complement(5754..6509)
                     /locus_tag="M2A35_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCB7198448.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6924..9221
                     /gene="xdhA"
                     /locus_tag="M2A35_002152"
     CDS             6924..9221
                     /gene="xdhA"
                     /locus_tag="M2A35_002152"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCB7198449.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEIDEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            9232..10110
                     /gene="xdhB"
                     /locus_tag="M2A35_002153"
     CDS             9232..10110
                     /gene="xdhB"
                     /locus_tag="M2A35_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCB7198450.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            10107..10586
                     /gene="xdhC"
                     /locus_tag="M2A35_002154"
     CDS             10107..10586
                     /gene="xdhC"
                     /locus_tag="M2A35_002154"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCB7198451.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(10626..12404)
                     /locus_tag="M2A35_002155"
     CDS             complement(10626..12404)
                     /locus_tag="M2A35_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCB7198452.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            12883..14070
                     /gene="ygeW"
                     /locus_tag="M2A35_002156"
     CDS             12883..14070
                     /gene="ygeW"
                     /locus_tag="M2A35_002156"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCB7198453.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            14128..15324
                     /gene="dpaL"
                     /locus_tag="M2A35_002157"
     CDS             14128..15324
                     /gene="dpaL"
                     /locus_tag="M2A35_002157"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCB7198454.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            15382..16593
                     /locus_tag="M2A35_002158"
     CDS             15382..16593
                     /locus_tag="M2A35_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCB7198455.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            16646..18031
                     /gene="hyuA"
                     /locus_tag="M2A35_002159"
     CDS             16646..18031
                     /gene="hyuA"
                     /locus_tag="M2A35_002159"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCB7198456.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            18079..19011
                     /gene="arcC"
                     /locus_tag="M2A35_002160"
     CDS             18079..19011
                     /gene="arcC"
                     /locus_tag="M2A35_002160"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCB7198457.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(19052..20677)
                     /locus_tag="M2A35_002161"
     CDS             complement(19052..20677)
                     /locus_tag="M2A35_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCB7198458.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(20725..21495)
                     /gene="yqeC"
                     /locus_tag="M2A35_002162"
     CDS             complement(20725..21495)
                     /gene="yqeC"
                     /locus_tag="M2A35_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCB7198459.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            21598..22176
                     /gene="mocA"
                     /locus_tag="M2A35_002163"
     CDS             21598..22176
                     /gene="mocA"
                     /locus_tag="M2A35_002163"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCB7198460.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            22498..25596
                     /gene="ygfK"
                     /locus_tag="M2A35_002164"
     CDS             22498..25596
                     /gene="ygfK"
                     /locus_tag="M2A35_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCB7198461.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            25599..26927
                     /gene="ssnA"
                     /locus_tag="M2A35_002165"
     CDS             25599..26927
                     /gene="ssnA"
                     /locus_tag="M2A35_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCB7198462.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            26978..27757
                     /locus_tag="M2A35_002166"
     CDS             26978..27757
                     /locus_tag="M2A35_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCB7198463.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            27754..30624
                     /locus_tag="M2A35_002167"
     CDS             27754..30624
                     /locus_tag="M2A35_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCB7198464.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            30789..32189
                     /gene="xanQ"
                     /locus_tag="M2A35_002168"
     CDS             30789..32189
                     /gene="xanQ"
                     /locus_tag="M2A35_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCB7198465.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            32207..33523
                     /gene="guaD"
                     /locus_tag="M2A35_002169"
     CDS             32207..33523
                     /gene="guaD"
                     /locus_tag="M2A35_002169"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCB7198466.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            33559..34926
                     /gene="ghxQ"
                     /locus_tag="M2A35_002170"
                     /gene_synonym="ygfQ"
     CDS             33559..34926
                     /gene="ghxQ"
                     /locus_tag="M2A35_002170"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCB7198467.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(34962..35450)
                     /locus_tag="M2A35_002171"
     CDS             complement(34962..35450)
                     /locus_tag="M2A35_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCB7198468.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(35450..37369)
                     /gene="ygfT"
                     /locus_tag="M2A35_002172"
     CDS             complement(35450..37369)
                     /gene="ygfT"
                     /locus_tag="M2A35_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCB7198469.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            37805..39253
                     /gene="uacT"
                     /locus_tag="M2A35_002173"
     CDS             37805..39253
                     /gene="uacT"
                     /locus_tag="M2A35_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCB7198470.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            39255..39380
                     /locus_tag="M2A35_002174"
     CDS             39255..39380
                     /locus_tag="M2A35_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198471.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(39377..39448)
                     /locus_tag="M2A35_002175"
     CDS             complement(39377..39448)
                     /locus_tag="M2A35_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198472.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            39503..40051
                     /gene="idi"
                     /locus_tag="M2A35_002176"
     CDS             39503..40051
                     /gene="idi"
                     /locus_tag="M2A35_002176"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCB7198473.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(40094..41611)
                     /gene="lysS"
                     /locus_tag="M2A35_002177"
     CDS             complement(40094..41611)
                     /gene="lysS"
                     /locus_tag="M2A35_002177"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCB7198474.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(41621..42719)
                     /gene="prfB"
                     /locus_tag="M2A35_002178"
     CDS             complement(join(41621..42643,42645..42719))
                     /gene="prfB"
                     /locus_tag="M2A35_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCB7198475.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(42810..44543)
                     /gene="recJ"
                     /locus_tag="M2A35_002179"
     CDS             complement(42810..44543)
                     /gene="recJ"
                     /locus_tag="M2A35_002179"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCB7198476.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(44549..45259)
                     /gene="dsbC"
                     /locus_tag="M2A35_002180"
     CDS             complement(44549..45259)
                     /gene="dsbC"
                     /locus_tag="M2A35_002180"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCB7198477.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(45284..46180)
                     /gene="xerD"
                     /locus_tag="M2A35_002181"
     CDS             complement(45284..46180)
                     /gene="xerD"
                     /locus_tag="M2A35_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCB7198478.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLLKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            46292..46813
                     /gene="fldB"
                     /locus_tag="M2A35_002182"
     CDS             46292..46813
                     /gene="fldB"
                     /locus_tag="M2A35_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCB7198479.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(46853..47260)
                     /locus_tag="M2A35_002183"
     CDS             complement(46853..47260)
                     /locus_tag="M2A35_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCB7198480.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(47241..47507)
                     /gene="sdhE"
                     /locus_tag="M2A35_002184"
     CDS             complement(47241..47507)
                     /gene="sdhE"
                     /locus_tag="M2A35_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCB7198481.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            47750..48730
                     /gene="ygfZ"
                     /locus_tag="M2A35_002185"
     CDS             47750..48730
                     /gene="ygfZ"
                     /locus_tag="M2A35_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCB7198482.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
BASE COUNT        12300 a        12433 c        11649 g        12373 t
ORIGIN      
        1 tctatatttt cgaagctaac ataatcgtag cgacgatggg tagcataatt ttttcgtgct
       61 aattttttat ctgtgataat tggtattccg atttcctttg catatttcct tacgcctaag
      121 gccacctcat tagttactct tacagaaatc aatggaatag gtgaaagatg tggcttaaag
      181 tatatcccta ttgcaatgtg agtagggttg gaaatcatca aacgactatt actgacatca
      241 gacttcaatt gctcagaaag aatttcctga tgcgctctct gcgtttagac ttaatttctg
      301 gatttccttc ttgctccttg tattctcttt taacttcttg tttatccatt ttcatatctt
      361 tcataaataa aaaatattca gcgataaaat caataattaa gacaattatc atactgccga
      421 gacaatacag tatgagaagg aatagcatct ctccccaatc tgataataaa gatatgatat
      481 ctccatcaag agttttaaaa agcagtgatt tatgattact ccaaaatact ttgatcgcca
      541 atgcaaaaaa aacaatataa agaattgctt taacaaattc ttttacggtt tttaagccaa
      601 atatacgctt taagccattt actggattca atgaatcaaa cttaaattta acagcttcag
      661 ttgctaattg cattttactt tgaaaccagg agacaagtat tgttgtgaac atgcaaacca
      721 gcaaaaaagg aatgttcgtt ttcaaccaac caccccccat agctttaaaa tagtcccaaa
      781 tatctggttt agcgttatga tcaaggatgt attcaagaat cgacataatg tgatgcacat
      841 caaaaacata tccgagatat aaagtaccca caagcattat tacagagatg gttaagtctc
      901 tactttttag aatttgccct tttttagaag catcctggag tttcttttgt gttggctttt
      961 cagttttatt ggccatgaaa cactctattt ctcaatgtat agttgcaatt gttctgggaa
     1021 aaaggataac ggcattactc gttcagccag tattggggca aaataaataa ttaatattaa
     1081 aaatgccagc gcacttttga cagtcagtga aatggcaaaa gcatttagtt gtgaagcata
     1141 acatgccagc aatcccagga cagcctcacc gcccagcata acggcaatca cggggcttgc
     1201 gtaaacgata gtatgagtca ttatgttatt aatatatgag aataaaggct ctaatttagg
     1261 aaaattaaaa ttgcctgaag gccataaatt ataactttgc caaagagttt ccagaataaa
     1321 gttcattcca cctttagtaa gataaaccgc agcagagaaa aggttaaata atcttgctaa
     1381 ttccgaggta tcgacacctg tagccggatc caaagtacta cttaatgttg caccgcgttg
     1441 gttatcgata aagctaccta tagcaaggaa catccaaaac ggggatgcta ataagcatgc
     1501 aataaacagc cctatgataa tttctctggc agcgaacaaa gcaatatcga tttcatttaa
     1561 gttcaataat tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg
     1621 gtgacgaact actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg
     1681 tgccaatcgg caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagtat
     1741 cgcctccccc atgctatcct ctcgcgaatg ctaaattcag catttcgata ccaaatgaat
     1801 ataatttttc accataccac ccgctcatta aaaaaaagca aatacttacg cacaataatt
     1861 tcacgccaaa tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca
     1921 ataaaccaac aaatgtcgca acttcaatag gtcctgcaga cataactaaa atcagataca
     1981 gcgccctatt ccccgcaaaa acaatatcgt ccatattttt tcctcaggga ttaatggata
     2041 ggtcaaaata ttgtaaaatg agcccttttg ataacattgt ccaaccgtcc atcgcgacaa
     2101 acaaaattaa ttttatcggt gtagatatgg ttaccggact catcatcatc atacctaacg
     2161 ttaacagaac gctggatata accagatcca ctacgacaaa gggtaaatag atataaaaac
     2221 ctataataaa tgcacttttt atctcactca atgcatatgc gggcagtaac gagaaaattg
     2281 aaatattatc atcatcgatt atctcttcat tgtcttcaga actattcacc ttctgaatct
     2341 tttcaaaaaa gctaaccaac tcaggttcag aatattttat caaatatgat ttataaccac
     2401 tcatgcctgt ctcaacaaaa ttaacaaccg atgctacatt attgaatgaa agattctcat
     2461 tctgactgtt attatatatt tccttcccaa ccggcatcat tacgaacatt gacaatagca
     2521 aagcaacccc attaagtgtc atatttgatg gcacttgctg aagtcccagc gcgtttcgga
     2581 caataacaaa aacaatcgag aatttaataa aacatgtccc ggatgctatt ataaacggca
     2641 atagtgtaaa taatgataat atcgctatca gcgaaatgct atttgacatt attattccct
     2701 gataaccaag aatgaatttc tacgcccaat ttatcatcca cttcaacaag ttcgccatag
     2761 ccagttagcg caccatttac acgtatctct atatttttct cagattcagg aagcagagat
     2821 attattcttt ttgcacagag atcgtctatt tcatatagat tcattatttt cttaccaaga
     2881 acaaactcaa tttttacagg caactcttca aaactattaa tcatctgttc gtcattttca
     2941 tcataaatct cactctcgtt ttttttgata tcaaaatcat ctgtttcaaa atcttcctcg
     3001 tactcaaaat gatcagccat ttttaactcc tctggataaa ttaaatcaca aatttttgta
     3061 ttataaataa ccgcataagc caaattattt gagattaata aaacatcacc aacttcaata
     3121 tcaacaagac ttctgtaaca tgttttacta aatccaatta caaaatgaat ggaccaatat
     3181 aatgattttt tgtctaacac tggagcgttt ccgtattggc agactgtgac tttatctaac
     3241 caaagattca attcccctgt tcatattctt attagtggtt gagcagggtt caatactcca
     3301 tcaaatactt tacctacgtg caaacgatta tattttagtt caggatgctt tagatttaac
     3361 ggggaactgg ataaactgag ccattcccct attaaccttt ccgtttctgg cattaaagcc
     3421 atcccagcga gctcagatga aattaaggga aatatttctc caagcaatac ccatccagac
     3481 cagctgccac tctcttctgc tatcggtaac catttagcac taaattcggg cattaataat
     3541 gcaacatctt cgccattttc tttccaattt tgaaaagtgg tttcaaacgt atgagtgttt
     3601 cgatttactt tcctcagacc gaacattaac tttcatcctt ctgatcgttt tcgaatttgc
     3661 tttcattttc ttgctcctca taaggctgat gtcgttgtcc ttgtcgttca tcttgctcag
     3721 tcaataccca acgttgtgct gtaactcatt atgaaaacta tttaattccc tcatcattga
     3781 taaggttgga catctctttc aacatgagag tcattgaagc tttcgaccaa tttagcggaa
     3841 tgatagggct tggcgcttca tgcaaagttc ccgacttgtg gatatagtta tagctttcgg
     3901 gaagtgccat ttcaggtaca ctcctgccat ttgcaccaat catattctcg cctgttattt
     3961 gggccaatga tctattcata aattgcactg agtcattcaa atactcttcc tttcttgatt
     4021 ctttataaac aattgctgct gattttgcat accatgagtc aaaaaaccat tctgcttctg
     4081 ttgatggaat gaagctcttt tctcttttcg catgtgaatt ttgatcggta tcggttttaa
     4141 tatcattgaa ccaaaaattt gccgactgat aattatcctt ctcgtatctt tttataccat
     4201 acggacctgc tagtttttta actattttta gaacctgctc ttttcttctg gtgtttatct
     4261 ttgcaagatt tgcaggataa ataacattaa gtaaagccgc atcggcttct ctgtaatgtt
     4321 tatctttttc cagataacct ggggattctc cccctaaatc aagctgtaac gttattcttt
     4381 cataaccttt atctatgagg tggttcaacc tcgttgttga aagaggttca tccagttcat
     4441 tggctttagc ttcgcgtagt aaatcagaga caaaaacaga gtctttcttc gagagtaagt
     4501 tcgacaaacg ttccagtcca gaggtcacca gtgcaacgga agatgtgttt aatcgggcat
     4561 cttcttccca tgctccggaa tcttccataa catagaaatt ggctttatcc agataagcaa
     4621 tcagcagcgc aaccgatttt aatcggtcac ctttttgcca atcttcagcg ttaattgtat
     4681 cagtgtcgat tgcttgtata aggagatcaa gataaagacc taacgcatca ttttgtttat
     4741 ggttccacag ctgcggcttg ccttcttcct gcacatctgc catcacgggg gagttgctgt
     4801 caaatcggat atgcataaca ttcatttgcc cgggaactcc atccagtcgt ttggggttgc
     4861 taatgacgtc ctgcatccgc ttaatctggt cgggcgtaga catataatcc cacagggtga
     4921 gcagaacctt ttttgcggct acactatttc cctgatctga tactaatgcc atatagcccc
     4981 ataaactatc cctcagccag acagcatcat agtgggtttc tgccgctgtt ttgtcatctt
     5041 cgaaagtaga agcaagaata aacgttcccc agggtttctc cgtcaacgtt aactttcgtg
     5101 tgttttctgt taatgttagc aatccttgaa tttcgtcttt ggtatagtgg ctctttattt
     5161 cattttgtaa tttggctgag tgaatcaaaa gacgataatt aggatactta tctggattta
     5221 acgataaata acttttatct gttgctgccc ccgtggaatt aagaatgtta aatacagatt
     5281 cactcgtcac ttctgcccct gtcgcctggc atgtgccgca cgaaaatata ccagcgagca
     5341 gaagagttaa tgcagatttc cctttcataa aatattccat caatcgtttg ttaaaagcgt
     5401 agataaccac ttatttaaca acttgaaaat aaggtgtttt accagggttg ttacaaaagg
     5461 attgcattgc gtaaacgctt tttatttaca acaaaatggg gaagtattac ggcgattgcg
     5521 aatagtctga agcgattgaa ttaagataat atagaaaatc agatacagca aaaatggcag
     5581 attgatcagg tgaagataaa ttggagcggg cgaagggaat cgaaccctcg tatagagctt
     5641 gggaagctct cgttctacca ttgaactacg cccgcttcga gatgcgtaag gcattataaa
     5701 ccttacgctc tccttagcaa gtgccgcgct gctgactgct gattaattcg ccatcagcat
     5761 tttggcttgc tgccctgcgg cggcaagtaa cgtagcggat caattgccgt tgcacggtaa
     5821 cgaatctgga aatgcaggcg aacagatgcc gcatccgtgc tccccatagt ggcgattttt
     5881 tgcccagcct tcacgctttg cccattattt accagcatcg tgtcattatg ggcgtaagcc
     5941 gtaatgtaat cttcactgtg tttaatcatg atgagattac cgtagccacg cagctggttg
     6001 cccacataca ccacctttcc tgcacccgcg gcgtaaatag gtgtgccccg tggagctgag
     6061 atatcaatcc ctttattgcc gccatctgct gtcgaatacg gcatgataac tttccctgtc
     6121 gttggccata accaacaacg ttgccctact ggcggccagg aagatttcgg taccgctgat
     6181 gacggtgtaa ccgatgcggt tttggtcgtt gatttggcgg ttgatttacg tgtactactg
     6241 ctacttttcg ccccacccag ttttagtttc tgaccaactt caatggtgta agggggggaa
     6301 atgccgttca gtcgcgccag ttcttttacg ctggttcccg tggtgcgcga aatacgatat
     6361 agcgtatccc cccgtttcac ggtgtaaacg gagccggaat acgttcctgt atcggatgat
     6421 ttgctacccg aacagcccgc caaaagcagt ccaaccgata acaacatcac gatacccaga
     6481 gattttttat tcaggcgtcc cgcactcaaa atctttcctc gcttaaaaaa caagacgcct
     6541 tatcatagca gccagcctct tgatacccaa tctcttgctt ctggaaaaac gtggtaatag
     6601 agaaagaggt taatttcatt tggcaaaggg aagtatcatc agcaaaatat tcgctgtaga
     6661 ggtatcgaac ttcctgactt tttaccctat ttcccatcct gatgcgtaat aatcccaatt
     6721 tatcacattg attttatcgc cactcatatt gatacgtatc actgccccta ttcagagtcc
     6781 tgtttcgtat catatctgtg tttttttaac gtcatcacac ttcctacctc ccctaacgct
     6841 tatcgtcgtt tctggcgtaa atcttgcctg cttagactaa atctttgcca taagaaccaa
     6901 atgttcaaat cgaggggatt tctatggaag cgcgggaagc aaccgctacg ggtgaatcat
     6961 gcatgcgcgt cgatgccatt gctaaggtca ccgggcgggc acgatatact gacgattatg
     7021 ttatggcggg catgtgttat gcgaaatatg tacgtagccc tatcgcacat ggttatgccg
     7081 taagtattaa tgatgaacaa gccagaagtt tgccgggcgt actggcgatt tttacctggg
     7141 aagatgtgcc tgatattcca tttgctactg ctgggcatgc ctggacactt gacgaaaaca
     7201 agcgcgatac cgccgatcgc gcactgctaa ctcgccatgt tcgtcatcat ggtgacgccg
     7261 ttgccatcgt cgtggcccgc gatgaactca cggcagaaaa agcggcgcaa ttggtcagca
     7321 ttgagtggca agaattaccc gttatcacca cgccagaagc ggcgctggca gaagacgctg
     7381 caccaatcca taacggtggc aatttactga aacaaagcac gatgtcgacg ggtaatgtcc
     7441 aacaaacaat cgatgccgcc gactaccagg tacaggggca ctatcagacc cccgttattc
     7501 aacattgtca catggaaagc gtaacatcgc tggcgtggat ggaggatgac tcgcgaatta
     7561 ccatcgtttc cagcacccag atcccgcaca ttgttcgccg cgtggttggt caggcgctgg
     7621 atattccctg gtcatgcgta cgagtcatca aaccgtttgt cggtggcggt tttggtaata
     7681 aacaggatgt actggaagag ccaatggcgg cattcctgac cagcaagctt ggcggcattc
     7741 cggtgaaagt ttcccttagc cgtgaagagt gtttcctcgc aacccgtacc cgccacgctt
     7801 ttaccattga cgggcaaatg ggcgtgaacc gcgacggaac attgaaaggt tatagtctgg
     7861 atgttctgtc taacaccggc gcttatgcat ctcacgggca ctccatcgct tctgcggggg
     7921 ggaataaagt cgcttacctt tatcctcgtt gtgcctacgc ttacagttca aagacctgct
     7981 ataccaacct cccctcggct ggtgcgatgc gtggttatgg cgcgccacaa gtcgtatttg
     8041 ccgttgagtc tatgcttgat gacgccgcga cagcgttagg tattgatcct gttgaaattc
     8101 gtttacgcaa cgccgcccgc gaaggagatg ctaatccgct cacgggcaaa cgtatttaca
     8161 gcgcagggtt gccggagtgt cttgaaaaag gccggaaaat ctttgaatgg gaaaaacgcc
     8221 gtgcagaatg ccagaaccag caaggcaatt tgcgccgcgg cgttggcgtc gcctgtttta
     8281 gctacacctc taacacctgg cctgtcggcg tagaaatagc aggcgcgcgc cttctgatga
     8341 atcaggatgg aaccatcaac gtgcaaagcg gcgcgacgga aatcggtcag ggtgccgaca
     8401 ccgtcttctc gcaaatggtg gcagaaaccg tgggggttcc ggtcagcgac gttcgcgtta
     8461 tttcaactca agataccgac gttacgccgt tcgatcccgg cgcatttgcc tcacgccaga
     8521 gctatgttgc cgcgcctgcg ctgcgcagtg cggcactatt attaaaagag aaaatcatcg
     8581 ctcacgccgc agtcatgcta catcagtcag cgatgaatct gaccctgata aaaggccata
     8641 tcgtgctggt tgaacgaccg gaagagccgt taatgtcgtt aaaagatttg gcgatggacg
     8701 ctttctacca ccctgaacgc ggcgggcagc tctctgctga aagctccatc aaaaccacca
     8761 ctaacccacc ggcgtttggc tgtacctttg ttgatctgac ggtcgatatt gcgctgtgca
     8821 aagtcaccat caaccgcatc ctcaacgttc atgattcagg gcatattctt aatccactgc
     8881 tggcagaagg tcaggtacac ggcggaatgg gaatgggcat tggctgggcg ctatttgaag
     8941 agatgatcat cgatgctaaa agcggcgtgg tccgtaaccc caatctgctg gattacaaaa
     9001 tgccgaccat gccggatctg ccacaactgg aaagcgcgtt cgtcgaaatc gatgagccgc
     9061 aatccgcata cggacataag tcactgggtg agccaccaat aattcctgtt gccgctgcta
     9121 ttcgtaacgc ggtgaagatg gctaccggtg ttgcaatcaa tacactgccg ctgacgccaa
     9181 aacggttata tgaagagttc catctggcag gattgatttg aggataacat catgtttgat
     9241 tttgcttctt accatcgcgc agcaaccctt gccgatgcca tcaacctgct ggctgacaac
     9301 ccgcaggcca aactgctcgc cggtggcact gacgtactga ttcagctcca ccatcacaat
     9361 gaccgttatc gccatattgt tgatattcat aatctggcgg agctgcgggg aattacgctg
     9421 gcggaagatg gctcgctacg tatcggctct gcaacgacat ttacccagct aatagaagat
     9481 cctataactc aacgtcatct cccggcgtta tgtgctgcgg ccacgtccat tgctggaccg
     9541 cagatccgta acgtcgctac ctacggtgga aatatttgca acggtgccac cagcgcagat
     9601 tctgccacgc caacgctaat ttatgacgcg aaactggaga tccactcccc gcgcggtgtt
     9661 cgtttcgtcc cgattaatgg ctttcacacc gggccgggca aagtgtctct tgagcatgac
     9721 gaaatcctcg tcgcctttca ttttccgcca cagccgaaag aacacgcggg cagcgcgcat
     9781 tttaaatatg ccatgcgcga cgcaatggat atttcaacga ttggctgcgc cgcacattgc
     9841 cgactggata acggcaattt cagcgaatta cgcctggcat ttggtgttgc cgcgccaacg
     9901 ccgattcgct gccaacatgc cgaacagact gcacaaaatg cgccattaaa cctgcaaacg
     9961 ctggaagcta tcagcgaatc tgtcctgcaa gatgtcgccc cgcgttcttc atggcgggcc
    10021 agtaaagagt ttcgtctgca tctcatccag acgatgacca aaaaagtgat tagcgaagcc
    10081 gtcgccgcgg cggggggaaa attgcaatga atcacagcga aacaattacc atcgaatgca
    10141 ccattaacgg gatgcctttt cagcttcacg ccgcaccagg cacgccgctc tcggaattac
    10201 tccgcgaaca aggactgcta agtgtcaaac aagggtgctg cgtgggtgaa tgtggtgcct
    10261 gtacggtgct ggtcgacggc acagcaatag acagttgctt ataccttgcc gcctgggctg
    10321 aaggaaaaga gatccgcacg ctggaaggtg aagcgaaagg cggaaaactt tctcatgttc
    10381 agcaggctta tgcgaaatcc ggcgcagtgc agtgcgggtt ttgtacgcct ggcctgatta
    10441 tggctaccac ggcaatgctg gcgaaaccac gcgagaagcc attaaccatt acggaaattc
    10501 gtcgcggact ggcgggaaat ctttgtcgct gcacggggta tcagatgatt gtaaatacag
    10561 ttctggattg cgagaaaacg aagtaaaagg atatccggcc tgaattcagg ccggattcac
    10621 tgaggttatg tgtttaacaa ctcatatttc ttaatcttgc gatagagcgt agcaatgccg
    10681 atgcccagtt catcagcaac ttgcttcttg ctgttatgac gtgaaagcgc ctcgcggatc
    10741 atttgctttt ccatctcctc cagcgccgtg ccgcccgcat catcgagtga caggtgcgcc
    10801 tcactgacct ctgttacatc actttgctcc gttgtgccat tattcagcag atttggcggc
    10861 aatagcgtgc tgtcgataac ttcacctgaa ggaactacgt taaccagata ttccatcaaa
    10921 ttgcttaact cgcgcaggtt tccgggccaa cgatgcttac gcaatatttc gacgacatcg
    10981 ggagcaatgc caggataaac cgatcccaga cgacgggtat gcagatgtaa aaagtaatgc
    11041 accaatagtt caatatcttc ctgacgttca cgcagcggtg gcagagttat cgggataaca
    11101 ttaagtcggt agaagagatc ttcgcggaat ttaccttcgg caatgaactg ggccaaattc
    11161 tgattagttg cagaaatgat gcgaatgtcg acttgtattg ggctactggc accaatcggc
    11221 agaatttcac gcgcctcaat agcgcgcagt aatttagcct gcaacattaa tggcatatca
    11281 cctatttcat cgagaaacag cgtgcccgta ttcgccgcct gaatcaaccc tgttttaccg
    11341 ttggcagaag cgccagtaaa tgcgccttta acataaccga acagttcgct ttccagaagc
    11401 tgctccggaa tcgcggcaca gttgatagca ataaagggtt tattccgtct tccgctcaac
    11461 ttatggattg ctcgggcgac gacttcttta cccgtgccgc tttcaccaac caccataacg
    11521 ctggatgggc tgggtgcaat acggctaatg agtcgtttta attgccgcat aacacggcac
    11581 tcgccaacca attgttcaat atgtggttca tcaggtgcat ttgctacaga aaaactggta
    11641 tgcgattggt gaaatgccat taaaaataat tgtcggccct gaatgttatg caattgacca
    11701 atgattaatt cacttttatc gtcccacgaa acaatatgct gcatatgtcc atgagtaaaa
    11761 ttactctcaa atgttaatgg tctgaaacgg ataggtttcc caataatatt attttgtaca
    11821 acaccaagtg tttttaaggc agtctgatta acaaactgaa cccgactttc atcatcaaca
    11881 actaatacgc cctgatccat attatcgatc atggtcgcga atattttact gatgttatct
    11941 cctggcccct gatcctccag aagtttcgaa acaaaaatgg tggatatatg gcgaacataa
    12001 tcagaaaatt cgcgtaaatt atcactgata tgctcttgtt gctcgtgggt aacggcaatc
    12061 aaacttatca ccccaacaca acgatcctgt aaaatgacag gcgtacccag aaatgctttt
    12121 tcgcggcaat tttctttact atcgcaacct tcgcaaaggg gatcgaagcg agactgtgtc
    12181 acaacttttt cagttttcgt ttccaggacg tggcggagca ggcgtgagtt gccgctcaac
    12241 tggcggccaa gaaacttccc atacgcgcca gttccggcga cgcgacacaa gttttcatca
    12301 acgatctcaa cctcaagctg caaaacgctg gcaagcattc tggcaaaacg ctgaattgtc
    12361 ggttgaattt gcatcaatac tgactgcgta gtagcaagct ccatagcttt accttccaga
    12421 cttacttaaa agtcgatcat tgaagacgtt gatggttcac agatcatgat gatattaact
    12481 caggcgaaat tggctttgat aaaaacataa gatttttatc actttctaat gaaattatgg
    12541 aagagatatc acatttctat atcaatatga gaattacggc ggtgagttta tcaaactgaa
    12601 gagagatagc ctgtcccttt atcttatttc tgatacttag cagtaaataa ataacgcgat
    12661 aagaaaagcc aatcgttttc gtattttaca aacaacccga agctggcatc aatttgtgat
    12721 caaccccaca cattatccgt caaattagtc ttttacagcc ccgtggataa ttctggcaca
    12781 cttattgtta gtcccaggta tagctgtgaa aacaccaatc actttggcaa gtcacagtga
    12841 aataaaccac tttgcctgtc attccactac cgggacttta tgatgaaaac tgttaatgag
    12901 ctgattaagg atatcaattc gctgacctct caccttcacg agaaagattt tttgttaacg
    12961 tgggaacaga cgccagatga actgaaacaa gtactggacg ttgccgcagc attaaaagca
    13021 ctgcgtgctg aaaacatctc aaccaaagtc tttaatagtg gattaggtat ttccgtattc
    13081 cgcgacaact ccactcgtac ccgcttctct tatgcttccg cgcttaacct gctcggcctt
    13141 gcacaacaag atctcgacga aggcaaatca caaatcgctc acggcgaaac cgtgcgtgaa
    13201 accgccaata tgatctcctt ctgcgccgac gctattggta ttcgcgacga tatgtatctg
    13261 ggcgcaggca acgcctatat gcgtgaagtt ggcgctgcac ttgatgacgg ttacaagcag
    13321 ggtgtactgc cacagcgtcc ggctttagtg aacctgcaat gcgatattga ccacccgact
    13381 cagtcaatgg ctgacctcgc gtggttacgt gaacactttg gttcactgga aaacctgaaa
    13441 ggtaaaaaaa tcgccatgac ctgggcctac tctccaagct atggcaaacc gctctctgta
    13501 ccacaaggca tcatcggtct gatgactcgc ttcggtatgg atgtcaccct ggcccatccg
    13561 gaaggctacg acctgatccc ggatgtagta gaagtcgcga aaaacaatgc taaagcatct
    13621 ggcggtagct tccgtcaggt caccagcatg gaagaagcat tcaaagatgc agacatcgtt
    13681 tatccgaagt catgggcacc ttacaaagtg atggaacagc gtaccgaatt gctgcgcgct
    13741 aacgatcacg aaggcttaaa agcactggaa aaacagtgtc tggcacagaa cgcgcaacac
    13801 aaagactggc attgtactga agagatgatg gaactgaccc gtgatggcga agccctgtac
    13861 atgcactgcc tgccagctga tatcagcggc gtatcctgca aagaagggga agtgactgaa
    13921 ggcgtattcg aaaaataccg tatcgctacc tacaaagaag ccagctggaa gccttatatc
    13981 atcgccgcga tgatcctgtc ccgtaaatac gccaaaccag gtgcactgct cgagcaactg
    14041 ctgaaagaag cgcaagaacg cgtgaaataa cccctcgggc cgatcagctg gttggccctc
    14101 tatttccaga ggccaaaagg ataggatatg tccgttttct cattgaagat tgatatcgcc
    14161 gataacaaat ttttcaacgg cgaaacatca ccgctctttt cgcaaagcca ggccaaactg
    14221 gcgcgccagt tccaccagaa aatagctggt tatcgcccaa caccgctttg tgcgctggac
    14281 gatctcgcaa acctttttgg tgtgaagaaa attctcgtca aagacgaatc aaaacgattc
    14341 ggtctgaacg ccttcaaaat gcttggcggt gcgtacgcca tcgctcaatt attgtgtgaa
    14401 aaatatcatc ttgatatcga aacgctgtca tttgagcacc tgaaaaatgc catcggcgaa
    14461 aaaatgactt tcgcgaccac caccgacggc aaccacgggc gcggtgtggc gtgggcagca
    14521 cagcaactcg gacagaatgc ggtgatttac atgccgaaag gttctgctca ggaacgcgtt
    14581 gacgccattc tgaacctcgg tgccgagtgc atcgtcacgg atatgaacta tgacgatacc
    14641 gttcgcctga ccatgcaaca cgcgcagcag cacggctggg aagtggtaca ggacacggca
    14701 tgggaaggtt acaccaaaat cccaacctgg atcatgcaag gctacgcaac cctggcagat
    14761 gaagccgtcg agcaaatgcg tgaaatgggc gtaaccccga cgcacgttct gctgcaagcc
    14821 ggtgtcggag caatggccgg tggtgtgctg ggttatctgg tcgacgtcta cagcccgcaa
    14881 aatctgcaca gcattattgt tgaacctgac aaagctgact gtatttatcg ctccggcgtc
    14941 aaaggcgaca tcgtcaacgt tggcggtgat atggccacca tcatggcggg cctggcctgt
    15001 ggcgaaccta acccgctggg ctgggaaatc ctacgtaact gcgccaccca attcatctcc
    15061 tgccaggaca gcgttgccgc attaggtatg cgcgtgctgg gtaatccgta cggcaacgac
    15121 ccgcgcatca tctccggtga atccggcgct gtcggtttgg gcgttctcgc agcggttcat
    15181 tatcacccgc aacgtcaaag cctgatggaa aaactggcgc tgaacaaaga tgccgtggtg
    15241 ctggttatca gcactgaagg cgacaccgac gtgaagcact accgcgaagt tgtctgggaa
    15301 ggcaaacacg ctgtagcacc ttaatcaccc tattggaact actccctgca aaacggggag
    15361 taaaaaatct ggagaaaaat aatggctaag aatattccat tcaaactgat tcttgaaaaa
    15421 gcaaaagatt accaggcgga catgactcgc ttcctgcgcg acatggttgc tattcccagt
    15481 gaaagctgcg acgaaaaacg cgtagtacat cgtattaaag aagagatgga aaaagtcggc
    15541 ttcgataaag ttgaaatcga cccgatgggc aacgttctcg gttatatcgg ccacggcccg
    15601 cgtctggtgg caatggacgc tcatatcgat accgtcggca ttggcaacat caaaaactgg
    15661 gacttcgatc cgtacgaagg catggaaact gatgagctga tcggtggtcg cggtacttcc
    15721 gaccaggaag gcggcatggc atctatggtt tatgccggta aaatcattaa agacctcggt
    15781 ctggaagatg aatataccct gctggttacc ggtactgtgc aggaagaaga ctgcgacggt
    15841 ctgtgctggc agtacattat tgaacaatcc ggcattcgcc cggaatttgt ggtcagtacc
    15901 gaaccaaccg actgccaggt ataccgtggt cagcgcggtc gtatggaaat tcgtattgat
    15961 gttcagggtg ttagctgcca cggttctgcg ccagaacgcg gtgacaacgc cattttcaaa
    16021 atgggtccga ttcttggcga attacaagaa ctctcccaac gtctgggtta tgacgaattc
    16081 ctcggcaaag gcaccctcac cgtttctgaa atcttcttca catccccaag ccgttgcgct
    16141 gtagcagata gctgcgccgt ctctattgac cgccgtctga cctggggcga aacctgggaa
    16201 ggcgcgctgg acgaaatccg cgccctgcct gcagtacaga aagctaacgc ggttgtttct
    16261 atgtacaact acgaccgtcc gtcctggact ggcctggttt acccaaccga atgctacttc
    16321 ccgacctgga aagtggaaga agatcacttc accgttaaag cactggtgaa tgcctacgaa
    16381 gggctgtttg gcaaagcgcc ggttgttgat aagtggacct tctcaactaa cggcgtatct
    16441 atcatgggcc gtcacggcat tccggtgatc ggctttggcc cgggtaaaga acctgaagcg
    16501 catgcaccta acgaaaaaac ctggaaatct cacctggtga cctgtgccgc gatgtacgct
    16561 gcaatcccgt taagctggct ggcaacagag taattactct tcacttattc ccctccggtt
    16621 cgccggaggg tttttggagt ttgctatgcg cgtattgatc aaaaacggca ctgtcgttaa
    16681 cgcagatgga caagccaaac aggatttgct gattgaaagc gggattgttc gccagttggg
    16741 caacaatatt tcgccgcagc tcccgtatga agaaattgat gccactggct gttacgtttt
    16801 ccctggcggc gtggatgtcc atacgcattt caatattgat gtcggcatcg cgcgcagttg
    16861 tgatgatttt tttaccggta cccgcgcagc tgcgtgtggc ggtacaacaa ccattattga
    16921 ccatatggga tttggcccaa acggctgtcg gttacgccat caactggagg tttatcgtgg
    16981 ttatgccgcc cataaagcgg tcatcgatta cagctttcac ggtgtgatcc agcacattaa
    17041 tcacgcaatc ctcgacgaaa tcccgatgat ggtcgaggaa ggactgagca gttttaaact
    17101 ctatttaacc tatcaataca aactcaacga tgacgaggtt ttgcaggcat tacgccgtct
    17161 gcatgaatcc ggcgcgctga ccaccgtgca cccggaaaat gatgcggcta tcgccagcaa
    17221 gcgggcggaa tttatcgccg cagggttaac cgcgccgcgc tatcatgcct tgagtcgccc
    17281 tctggaatgc gaagcggaag ccatcgcccg catgattaac ctggcacaaa ttgccggtaa
    17341 cgccccgctc tatatcgtgc acctgtctaa cggcttaggt ctggattatc tgcgtcttgc
    17401 ccgtgcgaat caccagccag tctgggttga aacctgccca caatatctcc tgttggacga
    17461 acgcagttac gatacagaag acggcatgaa gttcattctt agcccaccgc tgcgtaacgt
    17521 acgcgagcag gacaaactgt ggtgtggcat cagcgatggt gcgattgacg tggtggcgac
    17581 cgatcactgc accttctcga tggctcaacg cctgcaaatt tctaaaggcg atttcagtcg
    17641 ctgcccaaat ggcttacccg gtgtggaaaa ccgcatgcag ttactgtttt ccagtggcgt
    17701 gatgacggga cgtataacac cggaacgctt tgttgaatta accagcgcaa tgcccgccag
    17761 gctgtttggc ctgtggccgc aaaaaggatt attagcgccc ggttccgatg gcgacgtggt
    17821 gattatcgac ccacgtcaga gccaacaaat tcagcatcgc catctccacg acaacgccga
    17881 ctactcgcca tgggagggtt ttacctgtca gggcgcgatt gtcagaacct tatcccgtgg
    17941 tgaaacgatt ttctgtgacg gcacctttac aggcaaagcc gggcgaggtc gtttcctgcg
    18001 acgcaaaccg tttgtccctc ccgtgctcta accactgtca gggatctata cacaatcaag
    18061 aatgatgagt gaggaaaaat gagtaagaaa attgttctcg ccctgggcgg gaatgcgctg
    18121 ggcgatgacc tggccggaca aatgaaagcg gtaaaaatta cttctcaggc aattgttgat
    18181 ttaattgccc aaggacatga agttatcgtc actcatggta acgggccgca ggtgggcatg
    18241 attaaccagg cctttgaagc tgctgcaaaa actgaagcgc actcgccaat gctgccgatg
    18301 tctgtctgcg ttgccttaag ccagggttat attggttacg atctacaaaa cgccttaaga
    18361 gaagaactgc tttctcgggg cattaataaa ccggtagcaa cgctggttac tcaggtcgaa
    18421 gtcgacgcta acgatccggc attcctcaac ccgaccaagc cgatcggttc gttctttacc
    18481 gagcaagaag cagagcaact gacaaaacag ggttacactc tgaaagaaga tgctggtcgc
    18541 ggctatcgcc gtgttgttgc ctcgccaaaa ccggttgata ttattgaaaa agaaacggtt
    18601 aaagctctgg tagatgcagg ccaggtggtg atcaccgttg gcggtggtgg tattcctgtt
    18661 attcgtgaag gtaaccatct gcgtggtgcc agcgcggtta tcgataaaga ctgggccagc
    18721 gcccgtttag cagaaatgat tgatgccgat atgctgatca ttctgactgc ggtagaaaaa
    18781 gtggccatta acttcggtaa agagaatgaa cagtggctcg atcgcctgtc attaagtgat
    18841 gctgaacgct ttatcgaaga aggtcatttt gcgaaaggtt ctatgctgcc taaagtggaa
    18901 gctgccgcct cgtttgctcg ctctcgcgca ggtcgtgaag cactgattac cgtattgagc
    18961 aaagcgaaag aagggattga aggcaaaacc ggaacggtga tttgtcagta attttaaggc
    19021 cgggtgggta ataccatccg gccattttca tttaagccac ttccagcact tcttttgtcg
    19081 ctttcacgcc ctgatgcatc aacatcatta acgcctcaag tacgccgccg ccaatcgccc
    19141 gggctttatc agaaacgctg gagaaatcag cggtttcacc acgaggatcg atatcaccaa
    19201 ttttaaaacc accaaccact gccaagccgt cgttcaacaa gccacgcacc atccccgtca
    19261 acggtgcttt aatttcatgc tcaccaatcc aggcaatcac atcgccctct ttcaccagat
    19321 cgcctaattt cacgttggat cgcataatgc ctgcagcagg agcacggatc acccgtcggg
    19381 tggtatgccc cataatattg ccaggaacac cggtattctc ctgcgcacaa ccagagtaaa
    19441 tcacctgacc gagccagtgc ccgcgatttg tttcaattac cgcatgacaa tccttccctg
    19501 cagtaaagcc cggcccaagc gcgattgtta ctggtgccat atctgcccgc gttcccaaat
    19561 tctgtttcgc cagaatagcg tccaccacgc aaagcggttt cagttcatca agcaatgaac
    19621 aggcgggatc taccatcaca gggatgaatc cgcgttcggt aagtttcatc gcttccgcag
    19681 agctggttgc caggcgagca gtgacgcctt cgaccgtcat ttcgccatcg aacacggcct
    19741 gggcaaacgc caccgtacaa cgaatcactg tcggtttttc cacttccagc atgatcactt
    19801 taaaacccgc atgatacaga cgtagcgcca caccactggc gatatctccc gcaccgcgaa
    19861 tcaccaccag ctggtgtccg gaaggatgag aaggtttcat catcagccca cccggcgcgt
    19921 tatttttcac ctgtaatatt tctgccaaca cgctgatggc gatctcctgc ggcgtttccg
    19981 cacctatgtt gtaaccaacg ggcgcatgta aacgggcaat atgttcttca gccacgcctt
    20041 tctcacgcaa ttgacgcagg aaaagctgaa ccttgcggcg acttgccagc aaccccaacc
    20101 atgcaatggg ctgttcaatg agtttgtcga gggcttcacg atcctgatta ttcgtggcaa
    20161 tcaggacaaa attatcaggg cgaatatcca gtgcttccac tgctgcacca aacgactcag
    20221 catggagaag cgtggttgat ggagggaata gttcaggatt gaggctttcg cgataaatat
    20281 cggcaacggc aatatcaaat cctaatagcg ccgcactctg ggctatcgcc cggttgacat
    20341 gccccgcgcc aatcaacacc agacgtggac gcataccatg gacgctgata aatactgaca
    20401 ttgcacctcc acaatctgac ccgacagcat ccgcaccgtt acgagccata cgtccatgga
    20461 ataatcgcgg cttacgttcc tgcaatgcct gaagtgactc ttcaatcacc ttccgctcaa
    20521 ccattccgcc accaattgta ccgacgatag aaccatcggc gcgcactaac atttgtgcag
    20581 aatggcgggg agtcgagcct cggctatcaa caatttgcgc catcgcaaac ggacaatttt
    20641 gctcttcgag ttttgcagcc tctgtgaaaa tattcatacc aacctcaatg gatatccttt
    20701 cagtaacccg gaatacccgg gccgctaatt cacaaatctg cgcgcgattg caggatgttc
    20761 ttgtatatcg cccagccaga ttgcctctac gtcgtgttgt tgcagcggtt gaaggagctc
    20821 gctttgtgca atcgcattct cacattgaga aaaacggtta atgaaccaga ctcgccgaca
    20881 accttgcggt acgtttttaa acgccccctg agggtggcga accagcgcaa cgagatcgct
    20941 cagttgcaag gttgcatcag gtgttaaccc ggtaatatca gcaaactgcg accagcgatg
    21001 gacattttct gtgctcactt tcgcgcccaa agtatgtcct cccatcacag caatcacgca
    21061 gcaactgctt ttgggtatgc aaggttcgtg ctcatcaggc gcttttaacg gcattccacg
    21121 cgagccatct gcttcaatga gaattacgtc acactctggt cgttgcacca gtgcatcaat
    21181 cgcttctggc gtaaatccct gcacttttcc ctggttcgct ttccagctgt gaaaacaaaa
    21241 tgaaatgggg gatgtaagag acgcatgagg aagcatggcg ggatcacgac agaaaaccac
    21301 gggccagtga gacgttggca taaacatatg tgtcgtcgtc gtgattaaca cacgcctgcc
    21361 gctagcctgg agcagttctg ccagccaaaa aagcaagctg gttttccccc cagcaccaac
    21421 aacagaaatc acagtgggac gtttctgcgc acctaaatca atgactaatg ccgatgggtc
    21481 aactatactt ttcacgagtc tttatgacct ctctgggata aattatcccc aacttaatcc
    21541 atcaggaagt aacgcaatta tcaggcgtta ttagccccta taaataatgg aaccactatg
    21601 tcagccatcg actgtataat taccgctgcc ggattatcat caagaatggg gcaatggaaa
    21661 atgatgttac cctggcaaca ggggacaatt cttgatacaa gtatcaaaaa tgcgttgcag
    21721 ttttgtagcc gaattatttt agtcaccggc tatcgtggta atgaactgca cgaacgttat
    21781 gcgaaccaga gcaatatcac tattatccac aacccagatt atgcgcaggg tttactaaca
    21841 tcagtaaagg ccgcagtacc cgcagtgcaa acagaacatt gttttctcac ccacggtgac
    21901 atgccaaccc tcaccatcga tatttttaga aaaatctggt cgttacgaaa tgatggcgca
    21961 atactgccac tccataatgg catccccggc catccgattt tagtgtcaaa accatgcttg
    22021 atgcaggcaa tccaacgacc caatgtcacc aatatgcgtc aggcactcct tatgggagct
    22081 cattattccg tcgagataga aaatgcagaa ataattttag atattgatac tccggacgac
    22141 tttattacag cgcaaaaaag gtatactaaa atttagaaaa tgtagttaaa cgattgcgtt
    22201 caaatattta atctctccag cactctttat cataatgata aggaatcact gattttgaga
    22261 aaagagaaaa agtgataaat gggctgacaa taataatttt caggagtgat tttaattctc
    22321 atttggcacc tgtgagttca ttcacaaaaa gcctttaagt ttcgagccgc ctcacatttt
    22381 tttatatttc ccgccaaacc tggcaagagt ggtgcgattg ttgctctatc cccctaaacc
    22441 accggatttc tcaacaccgg tcactcaatg atatctgtat aagctaagga gagggttatg
    22501 ggggatatta tgcgtcccat tccgtttgag gaacttttga cgcgcatatt tgatgaatac
    22561 caacaacaac gctcaatctt tggtattccc gagcaacagt tttactcacc tgtaaaaggt
    22621 aaaactgtta gcgtcttcgg tgaaacctgt gccactcccg tcggccctgc cgctggcccg
    22681 cacacgcagc tcgcgcaaaa tattgtcact tcctggctga ctggcggacg cttcatcgaa
    22741 ctaaaaaccg tccaaattct tgaccgcctg gagctggaaa agccctgtat cgatgccgaa
    22801 gacgagtgct ttaacaccga atggtctacc gaatttaccc tgcttaaagc ctgggatgaa
    22861 tacctcaaag cctggtttgc cctgcacctt ctcgaagcga tgttccagcc ttctgattcc
    22921 ggtaaatcgt tcatctttaa tatgagcgtc ggttacaacc tcgaaggtat taagcaaccg
    22981 ccaatgcaac agttcatcga caatatgatg gacgcatctg accatccgaa attcgcgcaa
    23041 tatcgcgata cgctgaataa attactccag gatgacgcat ttttagcccg ccacggattg
    23101 caggaaaaac gcgaaagctt gcaagcctta cccgctcgca tccccaccag tatggtgcag
    23161 ggcgtcaccc tctccaccat gcacggctgt cctccgcatg aaatcgaagc catttgccgc
    23221 tacatgctgg aagaaaaagg gctcaacacc tttgtgaaac ttaacccgac cttactggga
    23281 tacgcgcgtg ttcgtgagat cctcgatgtc tgcggtttcg gttacatagg cttaaaagaa
    23341 gagtcatttg atcacgacct caagctgacg caagcactgg aaatgctgga acgcctgatg
    23401 gcactggcaa aagaaaaatc actcggcttt ggcgtaaaac tgactaacac tctcggcacc
    23461 atcaacaata aaggcgcact gcctggtgaa gagatgtata tgtcaggccg tgcgctgttc
    23521 ccgctctcca tcaatgttgc agcagttctc tctcgcgcct ttgacggcaa actgcccatt
    23581 tcttattccg gtggtgccag tcagctgact atccgcgata tttttgatac aggtattcgc
    23641 cctattacta tggcaaccga cctgctgaaa cctggcggct atttgcgctt aagtgcctgc
    23701 atgcgcgagc tggaaggctc cgacgcctgg ggacttgacc atgttgacgt cgaacgactg
    23761 aacagactgg cagcagatgc gttaaccatg gaatacaccc agaaacactg gaagccagaa
    23821 gagcgtattg aagtggcaga agacctgccg ctgaccgatt gctacgttgc cccctgtgtt
    23881 actgcctgcg ctatcaagca agatattccg gaatacatcc gtctgcttgg cgaacaccgc
    23941 tatgccgacg cgctggaact catctaccaa cgcaacgctc tgcccgccat taccggtcat
    24001 atttgcgatc accagtgcca atacaactgt acccgcctgg attacgacag tgcgctgaat
    24061 atccgcgaac tgaaaaaagt cgcgctggaa aaaggttggg atgaatataa gcaacgctgg
    24121 cacaaaccag ccggttctgg ttcacgccat ccggttgccg tgattggtgc aggtccggcg
    24181 ggtctggcag caggttactt ccttgccaga gcgggccatc cggttacgct gtttgaacgc
    24241 gaagccaatg cgggcggcgt ggtgaaaaat atcattcctc agttccgaat tccggctgag
    24301 ttaattcagc acgatatcga ttttgttgcc gatcacggcg tgaaatttga gtatggctgc
    24361 tcacccgatt tgaccgttga acagttaaaa aatcagggct tccactatgt tctgattgcc
    24421 accggcactg ataaaaatag cggtgtgaaa ctggcgggtg acaaccaaaa tgtttggaaa
    24481 tcactcccct tcctgcgtga atacaacaag ggtacagcgc tcaagctggg caaacatgtg
    24541 gtcgttgtcg gggcgggtaa caccgcaatg gactgcgctc gtgcggcgtt acgcgttcca
    24601 ggcgtagaaa aagcaacgat cgtttaccgt cgttcactgc aagagatgcc cgcatggcgc
    24661 gaagagtatg aagaagcgtt gcacgacggc gtggagttcc gtttcctgaa taatccggaa
    24721 cgtttcgatg ctgatggcac cttaaccttg cgcgttatgt cgcttggcga acccgatgag
    24781 aaaggccgtc gccgtccggt tgaaaccaac gaaacagtaa cactgcatgt agacagcctg
    24841 atcaccgcca ttggtgaaca gcaggatact gaagccctga atgcgatggg cgtgccgctg
    24901 gacaaaaacg gctggccaga cgtcgaccac aatggcgaaa ctcgtctgac tgacgtcttt
    24961 atgatcggcg acgtacagcg cggaccatcc tccattgtcg ctgctgtcgg aaccgcgcgt
    25021 cgggcgaccg atgccatcct tagtcgggaa aatatccgtt cccaccagaa cgataaatac
    25081 tggaacaacg tcaatccggc ggaaatctat caacgtaaag gcgatatctc tatcactctg
    25141 gtgaacagtg acgatcgtga cgcgtttgtc gcccaggaag ccgctcgctg cctcgaatgt
    25201 aactacgttt gcagcaagtg tgtggatgtc tgcccgaacc gcgccaacgt ctccattgcg
    25261 gtcccaggct tccagaatag attccagacg ctgcacctcg acgcttactg taacgaatgc
    25321 ggcaactgcg ctcagttctg tccgtggaac ggtaaaccat acaaagacaa aatcaccgtt
    25381 ttcagcctgg cgcaagactt tgataacagc agcaacccag gcttccttgt ggaagattgc
    25441 cgggtacgag tacgactgaa taaccaaagc tgggtgttaa acatcgacag cgaaggtcag
    25501 ttcaacaacg taccaccgga gctgaacgat atgtgccgca tcatcagcca tgtccaccag
    25561 catcatcatt atctgctggg ccgcgtggag gtgtaatcat gttgattctg aagaatgtca
    25621 ccgcagtgca gttacacccg gcgaaagtgc aggaaggcgt tgatatcgct atcgaaaatg
    25681 atgtgattgt cgctatcggc gatgccctga cgcaacgcta ccccgatgcc agctacaaag
    25741 agatgcatgg tcggattgtg atgccgggaa ttgtctgctc gcacaaccat ttttactcgg
    25801 ggctttcccg cggaattatg gcaaacatcg ccccctgccc ggatttcatc tcaacgctga
    25861 aaaatctctg gtggcggctc gatcgcgccc ttgatgaaga gtcgctctat tacagcgggc
    25921 tgatttgttc cctggaagcg attaagagcg gatgtacatc ggttatcgat caccatgcct
    25981 ctccggcgta tatcggcggg tcgctctcca cattgcgcga cgcattttta aaagttggcc
    26041 tgcgcgcgat gacctgtttt gaaactactg accgtaacaa cggcatcaaa gagttgcagg
    26101 aaggtgtaga agaaaacatc cgcttcgccc gtcagattga tgaggcgaag aaagcagcaa
    26161 ccgagccgta tctggtggaa gcacatatcg gtgctcacgc gccgtttacc gtgccagatg
    26221 ccggtctgga gatgctacat gaagccgtga aagccacagg ccgtggtttg catattcacg
    26281 ctgcggaaga cctttacgac gtttcctaca gtcaccactg gtacggcaaa gacctgctgg
    26341 cacgactggc gcaattcgat ctcatcgata gcaaaacgct ggtcgctcat gggctgtact
    26401 tgtcgaaaga tgacatcgcc ctactcaatc agcgcgatgc gttcctggtg cataacgccc
    26461 gttcaaacat gaacaaccat gtcggctaca accatcacct tagcgacatc cgcaatctgg
    26521 cgttgggaac ggacggcatt ggttcggaca tgtttgaaga gatgaaattt gccttcttta
    26581 aacatcgcga tgcgggtggt ccgctgtggc ctgacagttt tgccaaagcc ctgactaacg
    26641 gtaacgaact gatgagccgc aactttggcg cgaaatttgg acttctggaa gccggttaca
    26701 aagctgattt aaccatttgc gattacaact caccgacgcc gctgctggca gacaatatcg
    26761 ccgggcatat cgctttcggt atgggctcag gcagcgttca cagcgtgatg gtcaatggtg
    26821 tgatggtcta tgaagaccgt cagtttaact tcgattgcga ttccatttat gcacaagcca
    26881 gaaaagccgc tgccagtatg tggcgtcgga tggatgcgct ggcataaatg acagatgccc
    26941 tcttcccgca gggaagaggg ttaaataagg aaagaagatg attgaacaat ttttcaggcc
    27001 cgactctgtc gaacaggcgc tggaactgaa gcgccgctac caggatgaag ccgtctggtt
    27061 cgccgggggc agcaaactca atgctacacc aacccgtacc gataaaaaga ttgccatttc
    27121 cttacaggat ctggaactgg actgggttga ctgggataac ggtgcactgc ggattggcgc
    27181 aatgtctcgc ttgcagccac tgcgtgatgc gcgatttatt cctgcagcgc tgcgtgaagc
    27241 cctcggtttt gtttactcac gccatgttcg taatcagtcg accattggtg gtgaaatcgc
    27301 cgcccgccag gaagagtcgg tgctgcttcc cgtcctgttg gcactggatg ctgaactggt
    27361 ttttggcaac ggcgaaacgc tgtcaatcga ggactacctg gcctgcccat gcgatcgcct
    27421 gctaaccgaa attatcatta aagatccgta tcgcacctgt gcgacccgca aaattagccg
    27481 ttctcaggca ggtttaaccg tcgtgacggc agccgttgca atgacagacc acgacggtat
    27541 gcgaattgcg ctggatggcg tcgccagtaa agcactgcgt ctgcatgatg tcgaaaaaca
    27601 aaatctggaa ggcaatgcac ttgaacaggc tgtcgccaac gccattttcc cgcaggaaga
    27661 tttgcggggc agcgtggcct ataaacgcta tatcacggga gttctggtag ccgacctgta
    27721 tgccgactgc caacaggctg gggaggaagc cgtatgatca tccactttac tttaaatggc
    27781 gcgcctcagg agctaaccgt taatccaggc gaaaacgtgc aaaagctgtt gtttaacatg
    27841 ggaatgcact ctgtacgcaa cagtgatgat ggcttcgggt ttgccggttc tgacgcaata
    27901 atctttaacg gtaatatcgt taacgcgtcc ttgcttattg ccgcacagtt agagaaggca
    27961 gatattcgta ccgcagaatc tttgggcaaa tggaacgagt taagtctggt tcaacaggca
    28021 atggttgatg ttggcgtggt gcagtctggt tataacgatc cagctgcagc tctgattatc
    28081 accgatcttc tcgatcgcat cgccgcacct acccgcgaag agatcgacga cgcgctttct
    28141 ggtttgttca gccgcgatgc aggctggcaa caatactatc aggtcattga actggcggtt
    28201 gcacgtaaaa ataatccgca ggccaccata gatatcgctc cgactttccg tgacgacctt
    28261 gaagtcattg gcaagcatta tcctaaaact gatgccgcga aaatggtgca ggcgaaaccc
    28321 tgctatgttg aagaccgcgt aacggctgac gcctgcgtca ttaaaatgtt acgtagccca
    28381 cacgctcacg cactgattac tcatctggat gtcagcaaag ctgaagcctt accgggcgtc
    28441 gttcacgtta ttactcacct gaattgcccg gatatctact ataccccggg tggtcagagc
    28501 gcaccggaac cgtcaccgct tgaccgccgt atgttcggca agaaaatgcg tcacgtcggc
    28561 gatcgtgttg ctgcggtcgt cgcagaaagt gaagaaattg cgctcgaagc attgaagctc
    28621 atcgacgttg aatatgaagt gcttaagccg gtaatgtcga tcgacgaagc aatggcggaa
    28681 gatgcgcctg tcgtgcacga tgaaccggtg gtgtatgttg ctggtgcgcc agatactctg
    28741 gaagacgata acagccatgc agcccagcgc ggcgagcata tgatcatcaa cttcccgatc
    28801 ggttctcgcc ctcgcaaaaa tatcgccgcc agtattcatg gtcatattgg cgatatggac
    28861 aaaggctttg ccgatgccga tgtgatcatt gagcgaacct ataactcaac gcaggcacag
    28921 cagtgcccga ctgaaacaca tatctgcttt acccggatgg acggcgatcg tctggttatc
    28981 cacgcctcca cccaggtacc atggcactta cgccgccagg tcgcgcgcct cgtaggcatg
    29041 aaacagcata aagttcatgt cattaaagag cgagttggcg gcggttttgg ctccaaacag
    29101 gacatcctgc tggaagaagt gtgcgcatgg gcaacctgcg tgaccgggcg tccggtactg
    29161 ttccgctaca cccgtgaaga agagtttatt gctaacacct ctcgtcacgt cgcgaaagtc
    29221 accgtcaaac tgggagcgaa aaaagatggt cgcctgacgg cagtgaagat ggatttccgc
    29281 gccaacactg gcccttacgg caaccactca ctcaccgtac cgtgtaacgg accggcgctg
    29341 tcgctgccgt tatatccgtg cgataacgtc gatttccagg tcaccaccta ctacagcaac
    29401 atttgcccaa atggtgctta tcagggttat ggcgcaccga aaggtaactt cgctatcacc
    29461 atggcattag cggaactggc tgaacagtta cagatcgacc aactggaaat tatcgaacgt
    29521 aaccgggtac acgaagggca agagctgaaa attctcggtg caatcggtga aggtaaagcg
    29581 ccgacctccg ttccttccgc cgccagctgc gcactggaag agatcctgcg tcagggtcgc
    29641 gagatgatcc aatggtcttc accgaaaccg caaaatagtg actggcacat cggtcgcggc
    29701 gtcgccatta tcatgcagaa atcgggtatc ccggatatcg atcaggctaa ctgcatgatc
    29761 aaactggaat cggacggcac ctttatcgtt cattctggcg gtgcggatat tggtactggt
    29821 ctggatactg tagtgacgaa actggcagca gaagtgctgc actgcccacc gcaggacgtg
    29881 catgttatct ccggtgatac cgatcatgcg ttgtttgata aaggcgcata tgcctcgtcc
    29941 ggtacttgct tctcgggtaa cgcggcgcgt ttggcagcgg aaaatctgcg ggagaaaatt
    30001 ctgttccacg gcgcgcaaat gttgggtgag gcagtggcag atgttcaact ggcaacgccg
    30061 ggcgtcgtgc gcggcaagaa aggcgaagtc agcttcgggg atattgctca taaaggcgaa
    30121 accggcaccg gctttggttc actggtggga actggcagtt atatcacgcc tgatttcgcc
    30181 ttcccgtatg gcgcaaactt cgctgaagtt gccgtcaaca cgcgtacggg tgaaatccgc
    30241 ctggataaat tctacgcctt gctggactgc ggtacaccgg tcaacccgga attagcattg
    30301 ggacaaatct acggtgctac cctgcgagct atcggccaca gtatgagcga agagatcatt
    30361 tatgacgccg aaggtcaccc gttaacgcgt gatttacgca gttacggcgc accgaaaatt
    30421 ggtgacattc cgcgtgattt ccgcgctgtg ctggtgccga gcgacgataa agtcggcccg
    30481 ttcggggcga aatcgatctc ggaaatcggt gtaaatggcg cagctccggc gattgctacc
    30541 gcaattcacg atgcatgcgg catctggtta cgcgaatggc atttcacacc ggagaaaata
    30601 ctcactgcgc tggaaaaaat ataaataata aatggccgct tcggcggcct gtttctctgt
    30661 ttggacgatg actaaatagt gactgcgaaa atgagataag ccgttctcaa ggcgtcctgc
    30721 gccccaaatt cgtgggaatt tattttacta attcagatga tcaaatttac tttaaaagga
    30781 gtgaagggat gtctgatata aacaatgcag gttctgacct tatatttgaa ctggaggatc
    30841 gccctccctt tcatcaggct ctcgttggtg ccattaccca tctgttggca attttcgttc
    30901 cgatggtaac ccccgcgtta atcgtggggg cggccttaca gctttccgct gaaacaactg
    30961 cctatcttgt ttctatggcg atgatcgcct ctggtattgg tacctggtta caagtaaacc
    31021 gctacggcat cgtcggttct ggcctactct caattcagtc agtcaatttt tcatttgtta
    31081 cggtcatgat tgcgctgggc agcagcatga aaagcgacgg ttttcacgaa gagttaatca
    31141 tgtcgtcgct tctcggcgtc tccttcgttg gcgcatttct ggttgtcgga tcttcattta
    31201 tcttgcccta tttacgtcgg gttattacgc ctaccgtcag cggtattgtg gtactgatga
    31261 tcggcttaag cctgattaaa gtcggcatta tcgattttgg tggaggattt gcagccaaaa
    31321 gcagcggtac attcggcaat tacgaacatc tcggcgttgg tttattggtt ttaattgtgg
    31381 tgatcggctt taactgctgt cgcagtccgt tgctacgcat gggagggatc gccattgggc
    31441 tatgtgtcgg ctatatcgca tcgttatgcc tgggcatggt ggatttcagc agtatgcgca
    31501 atttgccgtt aatcaccatc ccgcatccgt tcaaatacgg ctttagtttt agcttccatc
    31561 agttcctggt ggttggcacg atttatctgc ttagcgtgct ggaagctgtc ggcgatatca
    31621 ccgccacggc aatggtttcc cgccgaccca ttcagggaga agagtatcag tcccgactga
    31681 aaggcggcgt gctggcagac ggtctggttt ctgttatcgc ctccgctgtc gggtcattac
    31741 ccttaaccac gtttgcgcaa aataatgggg ttattcagat gactggcgtc gcttcacgtt
    31801 atgtcgggcg aaccatcgcg gtaatgctgg ttatcctcgg tttgtttccg atgattggcg
    31861 gcttcttcac gaccattccc tcggcagttc tgggaggcgc aatgacgttg atgttttcca
    31921 tgattgccat cgctgggatt cgcataatca tcaccaacgg tttaaagcgc cgagaaacac
    31981 ttattgtcgc cacttcttta ggtttaggac ttggcgtctc ctacgatccc gaaattttta
    32041 aaatattgcc agcctctatt tatgtactag ttgaaaaccc tatttgtgct ggcgggttaa
    32101 ctgcgatttt attaaatatt atcctccctg gtggctaccg acaggaaaac gttctgcctg
    32161 gtattacctc agcggaagag atggattaac agtaaaggag tcaatgatgt caggagaaca
    32221 cacgttgaaa gcggtacgag gcagttttat tgatgtcacc cgtacggtcg ataacccgga
    32281 agagattgcc tctgcgctgc ggtttattga ggatggttta ttactcatta aacagggaaa
    32341 agtggaatgg tttggcgaat gggaagacgg aaagcatcaa attcctgaca ctattcgcgt
    32401 gcgcgactat cgcggcaaac tgatagtacc gggctttgtc gatacacata tccattatcc
    32461 gcaaagtgaa atggtggggg cctatggtga acaattgctg gagtggttga ataaacacac
    32521 cttccctact gaacgtcgtt atgaggattt agagtacgcc cgcgaaatgt cggcattctt
    32581 catcaagcag cttttacgta acggaaccac cacggcgctg gtgtttggca ctgttcatcc
    32641 gcaatctgtt gatgcgctgt ttgaagccgc cagtcatatc aatatgcgta tgattgccgg
    32701 taaggtgatg atggaccgca acgcaccgga ttatctgctc gacactgccg aaagcagcta
    32761 tcaccaaagc aaagaactga ttgaacgctg gcacaaaaat ggtcgtctgc tatatgcgat
    32821 tacgccacgc ttcgccccga cctcatctcc tgaacagatg gcgatggcgc aacgcctgaa
    32881 agaagaatat ccggatacgt gggtacatac ccatctctgt gaaaacaaag atgaaattgc
    32941 ctgggtgaaa tcgctttatc ctgaccatga tggttatctg gatgtttacc atcagtacgg
    33001 cctgaccggt aaaaactgtg tctttgctca ctgcgtccat ctcgaagaaa aagagtggga
    33061 tcgtctcagc gaaaccaaat ccagcattgc tttctgtccg acctccaacc tttacctcgg
    33121 cagcggctta ttcaacttga aaaaagcatg gcagaagaaa gttaaagtgg gcatgggaac
    33181 ggatatcggt gccggaacca ctttcaacat gctgcaaacg ctgaacgaag cctacaaagt
    33241 attgcaatta caaggctatc gcctctcggc atatgaagcg ttttacctgg ccacgctcgg
    33301 cggagcgaaa tctctgggcc ttgacgattt gattggcaac tttttacctg gcaaagaggc
    33361 tgatttcgtg gtgatggaac ccaccgccac tccgctacag cagctgcgct atgacaactc
    33421 tgtttcttta gtcgacaaat tgttcgtgat gatgacgttg ggcgatgacc gttcgatcta
    33481 ccgcacctac gttgatggtc gtctggtgta cgaacgcaac taataataaa actttaacat
    33541 cctcgtgagg acatcattat gtctggagac atcctacaaa caccggacgc accaaagcca
    33601 cagggcgcgc tggataatta ttttaaaatt accgctcgtg gcagtaccgt tcgtcaggaa
    33661 gtactggctg gcttaacgac ctttctggcc atggtttatt ccgttatcgt cgttccggga
    33721 atgctgggca aagcaggttt tcctcccgca gctgtgtttg ttgccacctg tctggtcgcg
    33781 ggcttcggct cgttgctgat ggggttgtgg gccaatttgc caatggcgat tggttgtgcg
    33841 atttccttga cggcatttac cgcattcagt ctggtactcg ggcaacaaat tagcgttcct
    33901 gtcgcactgg gcgcggtctt tctgatgggc gtcatcttca ccgctatttc cgtaaccggt
    33961 gtgcgtacct ggatcttacg taatttaccg atgggtatcg ctcacggtac aggtatcggt
    34021 atcggcctgt ttctgctgct gattgctgct aacggtgtgg gtatggttat caaaaacccg
    34081 attgaaggct tgccagtggc gctcggtgcg tttacctcct tcccggtgat gatgagcttg
    34141 ctggggctgg cggtcatctt cggtctggag aagtgccgcg tacccggcgg gatcttgttg
    34201 gtgattatcg caatttcgat tatcggctta atctttgacc cagcggtgaa ataccacggt
    34261 ctggtggcga tgccaagtct gactggcgaa gatggtaagt ctctgatttt cagcctcgat
    34321 attatgggcg cactccagcc aaccgtactt ccgagtgtac tggcattggt gatgaccgca
    34381 gtgttcgacg ccactggcac catccgtgcc gtcgccggtc aggcgaattt gttggataaa
    34441 gacaaccaga tcatcaatgg cggcaaagcc ctgaccagtg actcagtaag ttcaatattc
    34501 tccggtctgg tgggcgcagc gcccgcagcg gtttatatcg aatcagcggc aggaaccgcc
    34561 gccgggggta aaacagggtt gaccgcaacc gtagtggggg cgttattcct gctgattctg
    34621 ttcttatcac cgctgtcatt tttgatccct ggttacgcca ctgcacccgc tctgatgtac
    34681 gtgggtttgc tgatgttaag taacgtctcg aagctggatt tcaacgattt tattgacgca
    34741 atggctggcc tggtgtgtgc cgtgttcatc gttctgactt gtaatatcgt taccggtatt
    34801 atgctgggct ttgtgacact ggtcgtaggc cgtgtctttg ctcgcgaatg gcaaaagttg
    34861 aatattggta cggtgatcat tactgccgca ctggtcgcat tttacgcggg tggttgggca
    34921 atctaataga ttctccgcgc ctttcagcgc ggagatttct ttcacagagg attctcaccg
    34981 ctggcaacaa tacgctgctg gcggactcgc ctgagttctc tttcagtaag caactgcagc
    35041 gcctgtgtcg ggcaagattc aacacaggct ggcccctctt cccgctgttc acagagatcg
    35101 catttcacaa tttgttgtcg ggtatccccc ggcaacgact gaatggtgat cattccaaac
    35161 gggcacgcac tgacgcaact ctgacagcca atacaacggg cagaattggt ctgcaccacc
    35221 tgctcgccca tcgtcagcgc ccccacgggg caagcgccaa cacaaggggc gttttcacac
    35281 tgatggcaca tcaccggcgc actgatgctg tccagtcgct gtaccttcag ccggggtaaa
    35341 aagacatcgg cattcaactc ctgttctgaa gggtgagcga ccacacaggc tacttcacaa
    35401 gtacggcagc caatacagtc agccggatta acgataatta acgatttcat cacgatgcct
    35461 tcgtatcaaa cagagttaac atatcgcgcg ccgcctgcct tcctgcggcc attgcagtga
    35521 caaccagatc cgcgccatga actgcatcac caccagcaaa gacttttttc agatgcgtct
    35581 gggtaggtaa atacccgacg tcaccggttt gaatcaggcc ccatttatcg agtttaattc
    35641 cgctgccctg caaccacggc atggcatgcg cctggaaacc aaaggccata atcagaacat
    35701 cggcgggcaa ttcaaactct gaacccgcta ccggacgagg acgacggcga ccatccggcc
    35761 ccggctcacc catggcggta cgaatcaggc ccaccgcagt taaacgccca tcttcgtcac
    35821 aagcgatata ttgcggttga acattgaact gaaactcgac accttcctcg cgcgcattaa
    35881 ccacctcttt gcgcgagccc ggcatactga cttcatcacg acgatacgcg caggtcacgc
    35941 tggcggcatt gaggcggatg gaagtccgca aacaatccat tgtcgtatcg ccaccgccca
    36001 ataccacgac tcgcttacct tccacgtccg tcagcggata ctcttcagac tccggcaatc
    36061 ccatgagctg gcgggtatgg gcagtcagga acggtagagc ctgaatgaca ccgggcgcat
    36121 cttcatgcgg cagatctgct cgcatcatcc cgtaagtccc cacgccgatg aaaactgcat
    36181 catattcaga agttaaatcg ctaaaggtaa tatcgcggcc aatttcacag ttaagatgaa
    36241 agtcgattcc cattgcggtg aatatctctc gccgctggct taataccgtt ttatcgagtt
    36301 tgaaaggagg aatgccaaaa gtcagcatac cgccaatttc tggatggcga tcaaagacat
    36361 caacctgaac tcccgcgcgc gccagaatat cagcacaccc taaccctgct ggcccagcgc
    36421 caatcaccgc cactttttcg ctacggggaa caactttgct gacatcggga cgccagccca
    36481 tcgccagcgc ggtatcggtg atgtagcgtt ccagattacc gatagagact gcgcccgagt
    36541 gatctttcaa agtacatgcg ccttcacaaa gacggtcctg tggacatacc ctgccgcaaa
    36601 tttctggtaa ggaactggtc tggtggcaaa gttctgccgc ttcaataatc tttccttcct
    36661 gtaccagacg gatgtaatcc ggaatagcgt tatgcagcgg gcaatgccag ttgcagttag
    36721 ctttttcggc acaataaaca cagcggtcac tctcataagt cgcttgcagt ggatccagcc
    36781 cgcaatatat ttcgccaaag tgggttttcc gttcactcgc tgaaatttta tctgcgcctt
    36841 tacgcgagtt aacggggagc aacgctgcac tgcgagatgg ctgagcatct gatgacgctt
    36901 ttcctgctgc cgttttgcgc tggcgagcca cctttatcca ctgtaaccct ttatcgtcca
    36961 tcagtcgtaa cgcctgtgtt gggcagactt caatacaggc ttgcgtgccg gaactgcgct
    37021 ggttacaaag gtcgcatttc tgcgcaatcg tatcgaccat ctcaacgacg ccaaaggggc
    37081 aagcgattgc acatctttta cagccaatac atttttgctc gtccagttgt acgctatcgg
    37141 actggaaagt cagagcatta accggacaag ccgtaacgca aggggcattg ttgcagtgat
    37201 ggcaggccac cggattcgca gcctggcctt tccctacaac gtggatacgc ggtcgaaagt
    37261 cactgtgact cagcggccag ttttcttgat tgtgtgccac cgcacaggca atttcacaag
    37321 catgacagcc tatacattcc gcagcttcag cagcgataaa cttattcatt tgcatccctt
    37381 tcatttaatg agttatgtct tctcaaattt cgcgatgcaa taaacgggca tatattaggt
    37441 agcatgaccg ttttaattat aattttgcga ctgagttcaa attattgccc ctcaatctat
    37501 gccgctttca ttatttcaac attgccgacc aaaagagcgc tatccagata aaaacgatta
    37561 aatgcgagag tgcgacatgc cagaatgatt aatgaaatat caatatgaga aaattacaga
    37621 taatttcatt gtcaggttct cttctcattt tcgtggttta agcacgatta tgtgattgcc
    37681 tccgcaattc ctgtctctaa ctccccttcc tcgcaaaaac tggcactcca cgagcatgtg
    37741 tttagacagt ttcattaacg taaacggttg ctttttactc tggcgggcga aaggagaaac
    37801 actgatgagc gccatagatt cccaacttcc ctcatcttct gggcaagacc gcccaactga
    37861 tgaggttgac cgcatattat caccaggaaa gctgatcata ctcggtctgc aacacgtcct
    37921 tgtcatgtac gcaggtgcag tcgctgttcc tcttatgatt ggtgaccgac tgggcctctc
    37981 aaaagaagct attgcgatgc tcattagctc ggatctcttt tgctgcggga tcgtcacatt
    38041 attgcaatgt atcggtatcg gccgctttat ggggatccgc ctgccggtga ttatgtcggt
    38101 gacctttgct gctgtaacac caatgatagc cattgggatg aacccggata tcggcctgct
    38161 ggggatattt ggtgccacta tcgccgcggg ttttatcacc acattattag cgccacttat
    38221 tggtcgcttg atgcctttat tcccgccact ggttaccggt gtggttatta cttctattgg
    38281 gcttagcatc attcaggtgg gtattgactg ggccgccgga ggtaaaggga atccgcaata
    38341 tggtaatccc gtttatttag gtatctcctt tgccgtctta atttttatct tgctcattac
    38401 tcgctatgcg aaaggattta tgtccaacgt cgccgtatta ctggggattg tatttggctt
    38461 tttactttcg tggatgatga atgaagtcaa tttatccggg ctacatgatg cctcgtggtt
    38521 tgcgattgtc accccgatgt cgtttggtat gccgattttc gatcccgttt ccattctgac
    38581 catgactgcc gtgttaatca tcgtgtttat cgagtcgatg gggatgttcc tggcactggg
    38641 tgaaatagtc ggtcgtaaac tctcttcaca cgatattatt cgcgggctgc gtgtcgatgg
    38701 cgtagggaca atgataggcg gcacgtttaa cagcttcccc cacacgtcat tttctcaaaa
    38761 cgttggcctg gttagcgtga cgcgcgttca tagccgctgg gtgtgtattt cttcgggaat
    38821 tatattaatc ctgtttggca tggtgccaaa aatggcggtg ctggtagcct ccattccgca
    38881 atttgtgctg ggcggcgctg ggctggtgat gttcggcatg gtactggcga cagggattcg
    38941 aattctgtcg cgctgtaact acaccaccaa ccgttacaac ctctatattg tggcgatcag
    39001 tctcggcgtt ggcatgactc cgacgctctc tcacgatttc ttttctaagt taccggccgt
    39061 actgcaaccg ttgctgcata gcggcattat gctcgcaacc cttagcgccg ttgtgctgaa
    39121 tgtcttcttt aatggctatc agcatcatgc tgacctggtg aaggaatccg tctctgataa
    39181 agatttaaaa gtcaggacag tacgtatgtg gcttctgatg cgcaagctga agaaaaatga
    39241 gcatggagaa taatatgaat tttttaatgc gcgctatatt cagtctgctg ttgcttttta
    39301 ctctctctat tcctgtcatt tctgactgtg ttgcaatggc cattgaaagt cgcttcaaat
    39361 atatgatgct acttttttaa atggttttta cctgtcggca tccgctcaaa acgggcggtt
    39421 gtcgataaac gctcacttgg ttaatcattt cactcttcaa ttatctataa tgatgagtga
    39481 tcagaattac atgtgagaaa ttatgcaaac ggaacacgtc attttattga atgcacaggg
    39541 agttcccact ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct
    39601 cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact
    39661 gagcaaaaaa gcgtggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg
    39721 agaaagcaac gaagacgcgg tgatccgccg ttgccgttat gagcttggcg tggaaattac
    39781 gcctcctgaa tctatctatc ctgactttcg ctatcgcgcc accgatccga atggcattgt
    39841 ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccacc agtgcgttac agatcaacga
    39901 tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc
    39961 cacaccgtgg gcgttcagtc cgtggatggt gatgcaggca gccaatagtg aagcaagaaa
    40021 attgttgtct gctttcgcgc agcacaatta aaaaaccccg acatttgccg gggttgtgag
    40081 cataacgtaa tgcttatttt accggacgca tcgccgggaa cagaataacg tcgcggatgg
    40141 tatggctgtt ggtgaacagc attaccatac ggtcgatgcc aatccccaga cctgctgtcg
    40201 gcggtaagcc atgttccagt gcggtaacgt aatcttcatc gtagaacatc gcttcgtcgt
    40261 cacctgcgtc tttcgcggca acctgatcca ggaagcgttg cgcctgatct tccgcgtcat
    40321 tcagctcgct aaagccgtta ccgatttcac gaccaccgat gaagaactca aagcggtctg
    40381 tgatttccgg gttaatgtcg ttacgacgcg ccagcggaga aacttctgcc ggatattcag
    40441 taatgaaggt cggctgaatc agatgtgctt ctgccacttc ttcgaagatc tcggtaacga
    40501 tacggcctag accccagctc ttctcaacgt ggataccgat agattcagca atcgctttcg
    40561 cagagtcgaa gttatccaga tccgccatgt cggtttccgg gcgatatttc ttgatcgctt
    40621 cacgcatggt cagtttttcg aacggtttac cgaagtccag cgtcacgtcg ccgtaggtca
    40681 cttccgtctt accgagaata tcctgtgcca gagtacggaa cagcgattcg gtcagctcga
    40741 tcagatcttt gtaatctgcg taagccatgt agagttccat catggtgaac tctgggttat
    40801 gacgtacgga aataccttcg ttacggaagt tacggttgat ttcgaataca cgctcgaagc
    40861 caccgaccac cagacgcttg aggtacagtt ccggcgcgat acgcaggtac atgtcgagat
    40921 ccagcgcgtt atggtgggtg ataaacggac gcgcagcggc accgcctggg atcacctgca
    40981 tcatcggcgt ttcaacttcc ataaagccgc ggttcaccat gaactggcga ataccagaga
    41041 ggatctgcga gcgtacttta aaggtgttac gggattcatc gttagagatg agatccagat
    41101 aacgctgacg atagcgcgct tcctgatcct gcaagccgtg gaatttatcc ggcagcggac
    41161 gcagtgcttt ggtcagcaga cgcagctcgg tacagtggat agacagttcg ccggttttgg
    41221 ttttgaacag tttacctttc gcgccgagga tgtcgccgag gtcccatttt ttgaactgct
    41281 cgttgtaaac gccttccggc agatcgtcac gggcaacgta cagctgaatg cggccaccca
    41341 catcctgcag ggtaacgaaa gacgctttac ccataatacg gcgggtcatc atgcggccag
    41401 caacggcgac ttcgatgttc agcgcttcca gttcttcgtt ctctttaccg tcgaattctg
    41461 cgtgcaattg gtcagaggta tgatcgcgac ggaaatcgtt cgggaaggca atcccctgct
    41521 cgcgcaggtt cgccagcttc tcacgacgcg ttttcagttc attgttaaga tcgactaccg
    41581 cgtcagcgcc ctgtgcgtgt tgttcagaca tgttggttcc tcataaccct gctttcaaac
    41641 ttgcttcgat aaattgatcc aggctgccgt ccagcacggc ctgcgtgttg cgggtttcta
    41701 ccccggtacg cagatcttta atgcgggagt catcaaggac ataagaacga atctggctgc
    41761 cccagccgat gtcggatttg ttatcttcca tcgcctgttt ctcggcattt ttcttctgca
    41821 tctccagttc ataaagcttc gctttcatct gcttcatggc ctggtctttg ttcttgtgct
    41881 gggaacggtc gttctggcac tgggtcacga tcccggtcgg gatgtgggta atacgcaccg
    41941 cagattcggt acggttaacg tgctgaccgc ccgcgccgga cgcgcgataa acgtcaatgc
    42001 gcaaatccgc cgggttgatt tcgatatcaa tatcatcatc aacttccggg taaacaaacg
    42061 cagagctgaa cgacgtgtgg cgacgaccgc cggagtcaaa cgggctctta cgcaccaggc
    42121 ggtgaacgcc agtttctgta cgcagccagc cgtaagcgta atcgccggag attttgatcg
    42181 tcacggattt aatacccgcc acttcacctt ccgactcttc gatgatttca gttttgaaac
    42241 cacgcgattc tgcccagcgc agatacatac gctcaagcat gctcgcccag tcctgtgctt
    42301 ccgtaccgcc agaccccgcc tgaatatcga ggtagcagtc ggcgctgtca tattcgccag
    42361 agaacatacg gcggaactca agctgcgcca gtttttcttc cagggcgtcg agttcagcaa
    42421 cggcttcgtt aaaggtttct tcgtcgtcag cttctacagc cagttccagc agaccagaaa
    42481 catcttccag cccctgtttc atttggtcga gggtgtcgac aacggcttcg agggaggaac
    42541 gctctttacc cagcgcctgt gcgcgttcgg gttcgttcca gacatccggc tgttccagct
    42601 cggcgtttac ttcttccaga cgctctttct tggcgtcgta gtcaaagata ccccctaaga
    42661 acgtcggagc gttccgtgag gtcctgaatg cgattattta ccggattaat ttcaaacatg
    42721 gtctgatttc ttttattgag ctagtcaaaa tgcggtgata agagcgggat tgtacccaat
    42781 ccacgctctt ttttatagag aagatgacgc taaattggcc agatattgtc gatgataatt
    42841 tgcaggctgc ggttgccgcg aaactcgttg atatcgagct tgtaagccag ttgcacttcg
    42901 cgcacgccgt tatccggcca gagagcggta tcgacattaa aagcaatacc atccagcagt
    42961 ggaccgccgc cgaccggttc gaccatcacc ttcaaatgac gttcgcccac cagccgctgt
    43021 tgcagcagac ggaaatgacc gtcaaacagc ggctccggga acatctgccc ccacgggcca
    43081 gcatcgcgca gcagctgcgc cacttccatg gtcatttcgg ccgggcttaa cggaccgtct
    43141 gacaccactt cgccttgcaa tagcgaaggg tccagccact cagtaaccag ttcgccaaac
    43201 cgttgttgaa agagttcgaa tttatcctct tccagcgaca aacccgccgc catcgcatga
    43261 ccgccaaact tcagcatcat gccagggtag agtgtgtcta atcgctccag tgcatcacgc
    43321 atatgcagcc cctgaatgga gcgacctgaa cctttcagcg taccatcacc tgctggcgca
    43381 aaggcgataa ccggacggtg aaaacgctct ttgatgcgcg aagccagaat accgacaacg
    43441 ccctgatgcc attcggggtg atacattgcc agcccgccgg gtagcgtgtc gcgacttcgc
    43501 tccagtttct cgcacagggt cagggcttca acttgcattc cttgttcgat ctcttttcgc
    43561 gtctggttta gcgcatcgag ttcatttgcc agcacgcgcg cttcgccgat gttgtcgcac
    43621 agcaagagcg ccacaccgac ggacatatcg tccagtcgtc cggcagcatt gagacgtggc
    43681 cccagcgcaa aacctaaatc gctggcggcg agtttttgtg catcacggtt tgccacttcc
    43741 agcagcgctt taatccctgg acggcacttt ccggcacgga tgcgactcat cccctgccag
    43801 gtcagaatgc gattattagc gtccagcggc acgacgtccg ccactgttcc cagcgcgacc
    43861 agatccagca gttctgccag gttaggaatt gcgatgccac gctcatcaaa ccagccctga
    43921 tcgcgcaaaa aggtgcgcag cgccagcatc agataaaacg ccacacccac gcctgccagt
    43981 gatttcgacg ggaaattaca gtcgcgcaag ttagggttaa tgatcgcttc cgctgcgggt
    44041 aatgtttcgc ccggcaaatg gtgatcggta acaataaccg gaatgcccaa cgagcgagcg
    44101 tgtacaaccc ccgcatggga ggaaataccg ttatccaccg tgacaattaa ctgcgcgcca
    44161 cgggcatggg cctgatcgac cacttccggg cttaagccgt aaccgtcttc gaaacggttt
    44221 ggtaccaggt agtcgatatt gctgcaacca agcgagcgca tcgccagcac gcttagagcc
    44281 gtgctggtcg cgccgtcggc gtcgaaatca ccgaccacaa taatccgcgt tccttcgcga
    44341 aaagcgttgt aaaggatctc aacggccttt tcgacaccgc tcagttgctg ccagggcaac
    44401 atacctttaa cactgcgttc cagttcttgc gcactgcgca cgccccggct ggcgtataaa
    44461 cggcgcagca agggaggcaa ttcagcgggc aagtctgccg tttcatcgac ttcacggcga
    44521 cgaagttgta tctgttgttt cacgcgaatt atttaccgct ggtcattttt tggtgttcgt
    44581 cgaggaattc tttcatctct ttcggcggct ggtaacccgg aacaagtgtg ccattgctca
    44641 gcacaactgc cggagtaccg ctaacgccaa gctggacgcc aagtgcgtaa tggtcggcaa
    44701 tatccacgtc gcagctggct ggtgcggcgc ttttacctgc catcacatca tcaaacgctt
    44761 tgtttttatc tttcgcacac cagatagctt tcatttcttt ctctgcatcg ctgtccagcc
    44821 cctggcgcgg gaaagcaaga taacgcacgg tgatccccag cgcgttatag tctgccattt
    44881 gctcatgcag tttgtggcag taaccacagg taatatcagt aaacacggtg atgacgtgtt
    44941 tttcctgcgg cgctttataa acgatcatct ctttttcaag cgcattcaac tgctttaaca
    45001 gcatcttatt ggtgacattg accggagccg tgccgctaac gtcatacatt ggcccctgaa
    45061 tgatatgttt accatcatcg gtgatgtaca acacgccgct gttagtcaga actgtcttca
    45121 tgccagctac gggcgcgggc tgaatatcgc tgcttttgat gcccattttg gctaacgttt
    45181 gttgaattgc cgcgtcatca gcctgagcaa aacctgaaaa cgccgctaac aaagtaaaca
    45241 acataaaacc tttcttcata aatcttcccg ttcttttcag acatcacgcc cgcgggtgat
    45301 gctgttgatg aagttgccgc agacgctcgg tagcgacatg cgtataaatt tgcgtggtgg
    45361 agaggtcgct gtggcccagt agcatctgca ccacgcgtaa atccgcacca tgatttaata
    45421 aatgggtggc aaaagcgtga cgcaatacat gcggtgacag cttttcgctg tcgatacccg
    45481 ccagcacagc ataatgttta atgcggtgcc agaaggtctg tcgcgtcatc tgctgcgcac
    45541 gctggctggg aaacaacacg tcaattgaca caccattcag cagccacgga cgcccatgtt
    45601 ccagataggt ttccagccag taaaccgcct cttcacctaa cggtaccagg cgctctttgt
    45661 tgcctttacc aatgacccgc actacgccct gacgcaggct gatatcactc attgtcagtc
    45721 cgaccagttc agagacacgc agcccggtag catacaacac ttcaagcatg gctttatcgc
    45781 gtagctccag tggctgatca attaatggtg cctgtaataa acgttcgacc tgcgcttcgc
    45841 ttaaatcttt tagcaaacgc tggggcaatt tcggtgaagc gagatgcgca ctgggatcgt
    45901 cttcacgaaa cttttcgcga taaagatact ggaacaatcg gcgcactgca ctcagcaaac
    45961 gcgctgagct ggtggcttta tacccgccct ccagccgttc tgccagtaat gcctgcaaat
    46021 cgtcactttg cgccgtcgcc agcgtcaacc cgcggtgatg caaccactcc accatcattg
    46081 acagatcgcg acggtaagcg ttcaacgtat tttcagccag atttttttcc agccacagag
    46141 catcaagaaa ctgctcgatg cgtgccagat cctgtttcac ttgcgcccct tatggtcact
    46201 catttgatcc attatgcctt attgtgccgt gactaaagcg attctgatac actagctgca
    46261 aaagccacag cagaatcgag aagcttacgt tatgaatatg ggtctttttt acggttccag
    46321 cacctgttac accgaaatgg cggcagaaaa aatccgcgat attatcggcc cagaactggt
    46381 gaccttacat aacctcaagg acgactcccc gaaattaatg gagcagtacg atgtgctcat
    46441 tctgggtatc ccgacctggg attttggtga aatccaggaa gactgggaag ccgtctggga
    46501 tcagctcgac gacctgaacc ttgaaggtaa aattgttgcg ctgtatgggc ttggcgatca
    46561 actgggatac ggcgagtggt tcctcgatgc gctcggtatg ctgcatgaca aactctcgac
    46621 caaaggcgtg aagttcgtcg gctactggcc aacggaagga tatgaattta ccagcccgaa
    46681 accggtgatt gctgacgggc aactgttcgt gggtctggcg ctggatgaaa ctaaccagta
    46741 tgaccttagc gacgagcgta ttcagagctg gtgcgagcaa atcctcaacg aaatggcaga
    46801 gcattacgcc tgatctcact gacggcttag cgcatatgct ttgccgtcat ctttatcttt
    46861 gcgtctcttg ttgcaacaaa atccgccgta aatcccgcca ttcggcttcg tccatgctgt
    46921 cggctgccag ccataaatgt tgccgtttac cgccatcaga acgtaaacgc agcatcatgc
    46981 cgctcttaat catccacggt gctttgacga tgcaccactc ctgcccttgc caacgcaaac
    47041 gcccgtccat caacaagcga atttccccct ggcgagcatt aatacgccgc tggctgcgaa
    47101 cgcaatcaaa caccaccagc gaaagtaaca ccatccataa cggggtgtaa ctgagtggcc
    47161 agggcatgag taaaataaca gcggcaacca gcccatgaat cagcaaggaa agccactgtg
    47221 cgcgccagga gacgcgcaaa tcagattgcc acaggaccac gttcccggtt ccgtgtctgg
    47281 atgagtcgga ccatcatttc cagttctgca tcggccggtt taccgtgatt catcagccag
    47341 ttaaacaggt ccggatcgtc acattccagc agacgaataa agatgcgttt ttcgtcatcg
    47401 cttaagctgt cgtactcatg ttcgaaaaac ggcatgattg aaatatcgag ttcgcgcata
    47461 ccacggcggc atgcccaatg aatgcgggct ttgttgttaa tgtccatctt cttcctgtct
    47521 cacgaaaatc cagtacccgg ctattgtaac gtgtttttcg ccttctttta cgggaatatc
    47581 agtaaacacc atcgcgatcg cgaaattaat tcacaacaat tcaatggctt catttttttg
    47641 gaagtcgcct cgcagaaggc acagatcgcg tagtgaaagc acttgcattg cctcatagct
    47701 cttttaccat tagtcattaa tacgccgtta agcaactcag gactctatta tggcttttac
    47761 accttttcct ccccgtcagc ctacggcttc tgcccgttta ccgctgacgc tgatgacgct
    47821 tgatgactgg gcgcttgcca ccattactgg cgcggacagc gaaaaatata tgcagggtca
    47881 ggtgacagca gatgtcagcc agatggcaga agatcagcac ctgctcgccg cccattgcga
    47941 cgccaaaggt aaaatgtgga gcaatttacg tctgttccgc gacggcgatg gctttgcatg
    48001 gattgaacgg cgcagcgtgc gtgaaccgca gctgactgaa ctgaaaaaat atgcggtatt
    48061 ctctaaagtg accatcgcgc cagacgacga gcgtgtgctg cttggtgttg ccggttttca
    48121 ggcgcgcgcc gcgctggcaa atctctttag tgaactgcct tcgaaagaaa aacaggtagt
    48181 taaagaaggc gcgaccactt tgctatggtt tgaacacccg gcagaacgtt tcctgatcgt
    48241 aaccgatgaa gctactgcta atatgctgac cgataaactg cgcggtgaag cggaactgaa
    48301 caatagccaa cagtggctgg cattaaacat tgaagcgggt ttcccggtga ttgatgccgc
    48361 caacagcggg cagtttatcc cacaggcgac caacctccag gcgctgggcg gcatcagctt
    48421 taaaaaaggc tgctataccg gacaagagat ggtggcgcga gcaaaattcc gtggtgccaa
    48481 taaacgtgcg ctctggttgc tggcaggtag cgccagccga ctgccggaag ctggtgaaga
    48541 cttagagctg aaaatgggcg agaactggcg tcgtaccggt acggtgctgg ctgcggtaaa
    48601 actggaagat ggtcaggtcg tggtacaggt cgtcatgaat aacgatatgg aaccggatag
    48661 catcttccgc gtacgcgacg atgcgaatac actgcatatc gagccgctgc cgtattcgct
    48721 cgaagagtaa atctctttat cgcatcaggc attta
//
LOCUS       DAHBHC010000031        47037 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHBHC010000031 DAHBHC010000000
VERSION     DAHBHC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.031"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="M2A35_002186"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="M2A35_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCB7198483.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="M2A35_002187"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="M2A35_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCB7198484.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="M2A35_002188"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="M2A35_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCB7198485.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="M2A35_002189"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="M2A35_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCB7198486.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="M2A35_002190"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="M2A35_002190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCB7198487.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="M2A35_002191"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="M2A35_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCB7198488.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="M2A35_002192"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="M2A35_002192"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCB7198489.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="M2A35_002193"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="M2A35_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCB7198490.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="M2A35_002194"
     CDS             complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="M2A35_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCB7198491.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9293..10138
                     /gene="glpF"
                     /locus_tag="M2A35_002195"
     CDS             9293..10138
                     /gene="glpF"
                     /locus_tag="M2A35_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCB7198492.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10161..11669
                     /gene="glpK"
                     /locus_tag="M2A35_002196"
     CDS             10161..11669
                     /gene="glpK"
                     /locus_tag="M2A35_002196"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCB7198493.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11805..12815
                     /gene="glpX"
                     /locus_tag="M2A35_002197"
     CDS             11805..12815
                     /gene="glpX"
                     /locus_tag="M2A35_002197"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCB7198494.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12912..13658
                     /gene="fpr"
                     /locus_tag="M2A35_002198"
     CDS             12912..13658
                     /gene="fpr"
                     /locus_tag="M2A35_002198"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCB7198495.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13663..14091)
                     /gene="uspD"
                     /locus_tag="M2A35_002199"
     CDS             complement(13663..14091)
                     /gene="uspD"
                     /locus_tag="M2A35_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCB7198496.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLCIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14118..14417)
                     /locus_tag="M2A35_002200"
     CDS             complement(14118..14417)
                     /locus_tag="M2A35_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCB7198497.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14629..15069)
                     /locus_tag="M2A35_002201"
     CDS             complement(14629..15069)
                     /locus_tag="M2A35_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCB7198498.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15170..15769
                     /locus_tag="M2A35_002202"
     CDS             15170..15769
                     /locus_tag="M2A35_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCB7198499.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15877..16644
                     /gene="tpiA"
                     /locus_tag="M2A35_002203"
     CDS             15877..16644
                     /gene="tpiA"
                     /locus_tag="M2A35_002203"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCB7198500.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16699..17454)
                     /gene="cdh"
                     /locus_tag="M2A35_002204"
     CDS             complement(16699..17454)
                     /gene="cdh"
                     /locus_tag="M2A35_002204"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCB7198501.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17561..18550)
                     /gene="sbp"
                     /locus_tag="M2A35_002205"
     CDS             complement(17561..18550)
                     /gene="sbp"
                     /locus_tag="M2A35_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCB7198502.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18870..19832)
                     /gene="pfkA"
                     /locus_tag="M2A35_002206"
     CDS             complement(18870..19832)
                     /gene="pfkA"
                     /locus_tag="M2A35_002206"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCB7198503.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20013..20915)
                     /gene="fieF"
                     /locus_tag="M2A35_002207"
     CDS             complement(20013..20915)
                     /gene="fieF"
                     /locus_tag="M2A35_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCB7198504.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21064..21564)
                     /gene="cpxP"
                     /locus_tag="M2A35_002208"
     CDS             complement(21064..21564)
                     /gene="cpxP"
                     /locus_tag="M2A35_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCB7198505.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21714..22412
                     /gene="cpxR"
                     /locus_tag="M2A35_002209"
     CDS             21714..22412
                     /gene="cpxR"
                     /locus_tag="M2A35_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCB7198506.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22409..23782
                     /gene="cpxA"
                     /locus_tag="M2A35_002210"
     CDS             22409..23782
                     /gene="cpxA"
                     /locus_tag="M2A35_002210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCB7198507.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23888..24562)
                     /gene="yiiM"
                     /locus_tag="M2A35_002211"
     CDS             complement(23888..24562)
                     /gene="yiiM"
                     /locus_tag="M2A35_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCB7198508.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24711..25694)
                     /gene="kdgT"
                     /locus_tag="M2A35_002212"
     CDS             complement(24711..25694)
                     /gene="kdgT"
                     /locus_tag="M2A35_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCB7198509.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25954..26574)
                     /gene="sodA"
                     /locus_tag="M2A35_002213"
     CDS             complement(25954..26574)
                     /gene="sodA"
                     /locus_tag="M2A35_002213"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCB7198510.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26859..27893
                     /gene="rhaT"
                     /locus_tag="M2A35_002214"
     CDS             26859..27893
                     /gene="rhaT"
                     /locus_tag="M2A35_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCB7198511.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27890..28756)
                     /gene="rhaR"
                     /locus_tag="M2A35_002215"
     CDS             complement(27890..28756)
                     /gene="rhaR"
                     /locus_tag="M2A35_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCB7198512.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28812..29648)
                     /gene="rhaS"
                     /locus_tag="M2A35_002216"
     CDS             complement(28812..29648)
                     /gene="rhaS"
                     /locus_tag="M2A35_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCB7198513.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29936..31405
                     /gene="rhaB"
                     /locus_tag="M2A35_002217"
     CDS             29936..31405
                     /gene="rhaB"
                     /locus_tag="M2A35_002217"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCB7198514.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31402..32661
                     /gene="rhaA"
                     /locus_tag="M2A35_002218"
     CDS             31402..32661
                     /gene="rhaA"
                     /locus_tag="M2A35_002218"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCB7198515.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32903..33727
                     /gene="rhaD"
                     /locus_tag="M2A35_002219"
     CDS             32903..33727
                     /gene="rhaD"
                     /locus_tag="M2A35_002219"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCB7198516.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33737..34051
                     /gene="rhaM"
                     /locus_tag="M2A35_002220"
     CDS             33737..34051
                     /gene="rhaM"
                     /locus_tag="M2A35_002220"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCB7198517.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34352..34798
                     /locus_tag="M2A35_002221"
     CDS             34352..34798
                     /locus_tag="M2A35_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCB7198518.1"
                     /translation="MAVLTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            34809..36260
                     /locus_tag="M2A35_002222"
     CDS             34809..36260
                     /locus_tag="M2A35_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCB7198519.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36250..37320
                     /locus_tag="M2A35_002223"
     CDS             36250..37320
                     /locus_tag="M2A35_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCB7198520.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37320..39068
                     /locus_tag="M2A35_002224"
     CDS             37320..39068
                     /locus_tag="M2A35_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCB7198521.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39118..40158)
                     /locus_tag="M2A35_002225"
     CDS             complement(39118..40158)
                     /locus_tag="M2A35_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCB7198522.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(40311..41144)
                     /gene="fdhD"
                     /locus_tag="M2A35_002226"
     CDS             complement(40311..41144)
                     /gene="fdhD"
                     /locus_tag="M2A35_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCB7198523.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            41338..44388
                     /gene="fdnG"
                     /locus_tag="M2A35_002227"
     CDS             41338..44388
                     /gene="fdnG"
                     /locus_tag="M2A35_002227"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:41923..41925,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCB7198524.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            44401..45303
                     /gene="fdoH"
                     /locus_tag="M2A35_002228"
     CDS             44401..45303
                     /gene="fdoH"
                     /locus_tag="M2A35_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCB7198525.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENLEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            45300..45935
                     /gene="fdoI"
                     /locus_tag="M2A35_002229"
     CDS             45300..45935
                     /gene="fdoI"
                     /locus_tag="M2A35_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCB7198526.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            45932..46861
                     /gene="fdhE"
                     /locus_tag="M2A35_002230"
     CDS             45932..46861
                     /gene="fdhE"
                     /locus_tag="M2A35_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCB7198527.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
BASE COUNT        11436 a        12253 c        12224 g        11124 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagccgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta
      721 gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt agtggaagtg ctggacgttg
      781 agccggtatt tactcactcc gtctggcggt tactgctctg ggcggcagat tactatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccaggcg gtggatctga
      961 acagcctgaa acgctccccc aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa
     1021 tctggcgcga ccaggtcgcc acgctcgaat ttaatgatgc cgcgttgcag gcgctacgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg caaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagcgc ggcagatact ttttctgcct ggctgctggc gggcgttacc ggttccggta
     1261 aaacggaggt ttatctcagt gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt ggaagttctg cattccggcc tgaacgacag cgagcggctt tcggcgtggt
     1441 tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggcgtg attgtcattg atgaagagca cgacagctcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacgcccg cgctggaaac gttgtgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgcc gggcggggaa tgcgcgtccg gcaattcaac
     1741 acgtgctgga tttaaaaggt cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccaacaatc aggttatcct ctttcttaat cgtcggggtt
     1861 ttgcgcctgc gctactgtgc cacgactgcg gctggattgc cgaatgccca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagtcagc
     1981 gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgcttgcgc cgctgttccc tgatgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaagggg cgctggaaca gcaactggcg gaagtacatc
     2161 gcggcggtgc gaggattttg atcggcacac aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg tttcgctcag ctttacaccc aggtcgctgg tcgtgccggg cgtgcgggta
     2341 aacaaggcga agtggtgctg caaacgcacc atccagaaca tcctctgttg caaacgttgc
     2401 tctataaagg ctacgacgcc tttgccgaac aggcgctggc tgagcgacgg atgatgcaac
     2461 tgccgccatg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaac
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg
     2701 cgctcatcaa tacaataccg gattcccgca aggtgaaatg ggtactggat gttgatccga
     2761 ttgagggtta aaccgcttac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 tgtctcccga gcattaatga atcccgataa agtctcccag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt cgcaccggaa ctggagctac ctacagttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ctatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggta
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat taaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacaacgcca gcgtcaggcg cagcaattag cggagcagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggat
     4861 ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc tggcgaaaga
     5341 ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag aaaatactga
     5521 actgagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcgt ctgccaatcc gcgttgaact gcaggcgctg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaagcgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaactatca tcattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacag cgctggcatg
     7141 gtggcaaggt cacttcgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac tggttgcagt
     7381 ggcatgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttgag tgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaatatt aataacctgc gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 gctggtgtgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccattac tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccaatgc tggaacgtac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgtttta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag cccacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttctgaagat
     8521 ccgctggata ttgaatgata gaaaaggcac cgcaaggtgc cttttttctg cgttacctgt
     8581 tggcctacac agtaaagaaa ttacgcggaa gatgaagcgt aatcagacct cttccatgcg
     8641 acccagcagg gcctgcagac gttcctgcca gccgttctgc tgttctttca gatggttgtt
     8701 ctcacgctcc agctcttcgc gctgatgctg ggcattttga acttcctgcg acagtgagtt
     8761 gtttttttct ttcagctctt cgatttccat ctgcaacaga gtgatggtat caatcgcctg
     8821 ctgtactttt gcttccagtt tctcaaacac ttctaatgac attgtcatac ctctcctgaa
     8881 ttgcaaggcg ttgatggata aaaatcctcg tcccgattac cggtgacgcc ttaataaata
     8941 cgagcgcact ttagttagct ccgattgtat gaagccgcgc catcgctgtc cagcggcacg
     9001 ccttgcagat tacggtttgc cacacttttc atccttctcc tggtgacata atccacatca
     9061 atcgaaaatg ttaataaatt tgttgcgcga atgatctaac aaacatgcat catgtacaat
     9121 cagatggaat aaatggcgcg ataacgctca ttttatgacg aagcacacac attttaagtt
     9181 cgatatttct cgtttttgct cgttaacgat aagtttacag catgcctaca agcatcgtgg
     9241 aggtccgtga ctttcacgca tacaacaaac attaactctt caggatccga ttatgagtca
     9301 aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc ggtaccgggt tgttgatttt
     9361 cttcggtgtg ggttgcgttg cagcactaaa agtcgctggt gcgtcttttg gtcagtggga
     9421 aatcagtgtc atttggggac tgggggtggc aatggccatc tacctgaccg caggggtttc
     9481 cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa
     9541 gcgcaaagtt attcctttta tcgtttcaca agttgccggc gctttctgtg ctgcggcttt
     9601 agtttacggg ctttactaca atttattttt cgacttcgag cagactcatc acattgttcg
     9661 cggcagcgtt gaaagtgttg atctggctgg cactttctct acttacccta atcctcatat
     9721 caattttgtg caggctttcg cagttgagat ggtgattacc gctattctga tggggctgat
     9781 cctggcgtta acggacgatg gcaacggtgt accacgcggc cctttggctc ccttgctgat
     9841 tggtctactg attgcggtca ttggcgcatc tatgggccca ttgacaggtt ttgccatgaa
     9901 cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc
     9961 ctttaccggc ggcagagaca ttccttactt cctggtgccg cttttcggcc ctatcgttgg
    10021 cgcgattgta ggtgcatttg cctaccgcaa actgattggt cgccatttgc cttgcgatat
    10081 ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa caaaaagctt cgctgtaata
    10141 tgactacggg acaattaaac atgactgaaa aaaaatatat cgttgcgctc gaccagggca
    10201 ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa tatcattagc gtgtcgcagc
    10261 gcgaatttga gcaaatctac ccaaaaccag gttgggtaga acacgaccca atggaaatct
    10321 gggccaccca aagctccacg ctggtagaag tgctggcgaa agccgatatc agttccgatc
    10381 aaattgcagc tatcggtatt acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa
    10441 ccggcaagcc tatctataac gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg
    10501 agcatttaaa acgtgacggt ttagaagatt atatccgcag caataccggt ctggtgattg
    10561 acccgtactt ttctggcacc aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg
    10621 agcgtgcacg tcgtggtgaa ttgctgtttg gtacggttga tacgtggctt atctggaaaa
    10681 tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc ctctcgtacc atgttgttca
    10741 acatccatac cctggactgg gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga
    10801 tgctgccaga agtgcgtcgt tcttccgaag tatacggtca gactaacatt ggcggcaaag
    10861 gcggcacgcg tattccaatc tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc
    10921 agttgtgcgt gaaagaaggg atggcgaaga acacctatgg cactggctgc tttatgctga
    10981 tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg
    11041 gcccgactgg cgaagtgaac tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca
    11101 ttcagtggct gcgcgatgag atgaagttga ttaacgacgc ctacgattcc gaatatttcg
    11161 ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc ggcatttacc gggctgggtg
    11221 cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg
    11281 ctaaccacat tatacgcgcg acgctggagt ctattgctta tcagacgcgt gacgtgctgg
    11341 aagcgatgca ggccgactct ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag
    11401 tagcaaacaa tttcctgatg cagttccagt ccgatattct cggcacccgc gttgagcgcc
    11461 cggaagttcg cgaagtgact gcattgggtg cggcctatct tgctggtctg gcggtcggtt
    11521 tctggcagaa cctcgacgag ctgcaagaga aagcggtgat tgaacgcgag ttccgtccag
    11581 gcatcgaaac cactgagcgt aattaccgtt acgcaggctg gaaaaaagcg gtcaaacgcg
    11641 cgatggcgtg ggaagaacac gacgagtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccgatagtc ctgaaaccgt gcgggggcaa ccccgcacac atcaataatc ccccccttcc
    11761 cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg
    11821 ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc tggctacaaa tggttaggac
    11881 gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc
    11941 aggtcaacat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc
    12001 tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc
    12061 cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag
    12121 taggcgataa aggctgcttc ctcaatgcgc cggatatgta tatggagaag ctgattgtcg
    12181 ggccgggagc caaaggcacc attgatctga acctgccgct ggcggataac ctgcgcaatg
    12241 tagcggcggc gctcggcaaa ccgttgagcg aactgacggt aacgattcta gctaaaccac
    12301 gccacgatgc cgttatcgct gaaatgcagc aactcggcgt acgcgtattt gctattccgg
    12361 atggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt
    12421 acggtattgg tggcgcgccg gaaggcgtag tttctgcggc ggtgatccgc gcattagatg
    12481 gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc
    12541 gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag
    12601 tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc accggtatta
    12661 ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc
    12721 tgctgatccg cggcaagtca cgcaccattc gccgcattca gtccatccac tatctggatc
    12781 gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat ttgatcgatt gagccttcca
    12841 gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt taatcaaaac
    12901 aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg
    12961 acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca
    13021 ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc tatgttaact
    13081 cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc
    13141 cacgattggc ggcactgaaa ccaggcgatg aagtgcaggt ggttagcgaa gcagctggct
    13201 tctttgttct ggatgaagta ccggactgcg aaacgctatg gatgctggca accggtacag
    13261 cgattggccc ttatttatcg attctgcaac taggcaaaga tttagatcgc ttcaaaaatc
    13321 tggtcctggt tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag
    13381 aactggaaaa acgctacgaa gggaaactgc gcattcagac ggtggtcagt cgggaaacgg
    13441 cagcggggtc gctcaccggg cggataccgg cgttaattga aagtggggaa ctggaaagcg
    13501 cgattggcct gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga
    13561 tggtgcgcga tacacaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc
    13621 gccgaccggg ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg
    13681 cacgatgagc aaatccgcac tcatcttatt gatcatcccg cgatatgccg gcatcaaacg
    13741 gttgataaat gagtgatgat gaccacagac aaggaggtcg cactgctctt tttgcataat
    13801 ttccagcagt gtttccggca tttctccgcg ttcaatacac agttttgtct tcggccattg
    13861 aatatttttc gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat
    13921 atcttctgtt gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg
    13981 aattaacgtc aggtgagcgt cattatgtct ggcgagctcc agagctttat tcaccagtaa
    14041 ggcatcttct tcattcccgg aaattgccac gccaatgtgt ttataagcca tgtttaactc
    14101 cttctaaagc aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg
    14161 gcctggcatg agaaagtgaa ggccgcatca agctcaactt gcccctccag atccttaatc
    14221 ttgtaggcca cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt
    14281 ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta
    14341 cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta
    14401 tctacgacat ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa
    14461 gaataatgcg ccgttaaggg aagaacaaac aagtaatgat cttatggatt accatttttt
    14521 ttgatattgc ttaaggtgaa gagttcagaa ggattttccg ggcattgtca tttgcatcgt
    14581 ggaaataaca atcccggaaa aaaatagcag taagagatat attaaaggtt aattacttga
    14641 tttattgtcg gctttatact tcacatcctg ggtatcttta ccatatttat tttcgccttg
    14701 tgtgccaatg aacgctccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa
    14761 acgccccacc gcccattgcc agacacccgg taaaatcgcc cagttacccg ccagcagcat
    14821 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
    14881 aacggttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
    14941 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
    15001 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
    15061 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
    15121 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
    15181 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
    15241 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
    15301 ctccatcagc cagtttcgag aagactttaa cagccagaat cccagcctgc cactgaacga
    15361 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
    15421 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
    15481 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
    15541 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
    15601 gaaaaaactg cagtcgctgc taacggcggg gaaaaacaaa cgttattaca ccgagacaga
    15661 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
    15721 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
    15781 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
    15841 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
    15901 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
    15961 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
    16021 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
    16081 aacctgtccg gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct
    16141 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
    16201 atcgcgaaaa aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt
    16261 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
    16321 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
    16381 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
    16441 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
    16501 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agatatcgac
    16561 ggcgcgctgg ttggcggtgc ttctctgaaa gctgacgctt tcgcagtaat cgttaaagct
    16621 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
    16681 tttccttaac tcttcgcctt aacgcaaaat ctcacactga tgatcctgaa tttcctcggc
    16741 tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc
    16801 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggtacttc
    16861 ttctgccagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
    16921 caggtattca tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
    16981 cagattgtta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
    17041 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
    17101 cggctggccg tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
    17161 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
    17221 caacagatat tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt
    17281 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
    17341 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
    17401 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
    17461 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
    17521 tcacgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
    17581 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
    17641 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gccacctcag
    17701 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
    17761 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
    17821 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
    17881 tccccagttc attcgctgcc agcagagctt cgttttccca ggcgatcagt acatcgccaa
    17941 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
    18001 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
    18061 gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag
    18121 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
    18181 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
    18241 gtttgatcca ctctttatca atccgcccgc gttccgcaat tgcgtccacg tcataggcca
    18301 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
    18361 aaccaccgtg tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
    18421 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
    18481 gctgaatatc ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc
    18541 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
    18601 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgtgggtata tgttgaggga
    18661 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
    18721 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat
    18781 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
    18841 gtaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
    18901 tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc
    18961 tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg
    19021 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
    19081 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
    19141 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
    19201 gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaacc
    19261 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
    19321 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
    19381 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
    19441 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
    19501 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat cccacgtttt
    19561 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaagcgcgca
    19621 gaaccgagga acgtaccgcc acggttgatc atgtcagaaa cgctgtaacg gtctagctgt
    19681 accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac ttccagacct
    19741 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
    19801 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
    19861 taaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag
    19921 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
    19981 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
    20041 ctgggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg aaaacgccgt
    20101 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
    20161 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
    20221 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
    20281 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
    20341 tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac
    20401 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
    20461 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaacg ctgaaacgag
    20521 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
    20581 gtcatcggtg ttggagatat cagatgttga atacccgtca aaaacaggaa tagtgccgaa
    20641 ccggagataa acatactttg cgccagcgcc gcgagggact ctgctttacc gtgaccaaac
    20701 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
    20761 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
    20821 caccatgcaa aaattttaat cagcaatagc aacgaagcca tcgccgtcgc agcaatcgcc
    20881 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgc
    20941 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
    21001 aaaaccccca catcatgtgg gggaagacag ggatggtgtc tatggcaagg aaaacagggt
    21061 ttactactgg gaacgtgagt tgctactact caatagcttc aacgatgaac ttttttgcca
    21121 ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt ttctcgttta aaaccgcttg
    21181 ctgctccggc gttaacaggc gatacatttg gttgcggact tttgccatct caacctgacg
    21241 agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt
    21301 ttctgcggtg acaaggcgat gcattgtctc cagttcgcta acattaacag gaggctgttc
    21361 gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat
    21421 gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt
    21481 atcgcctgaa ccgacttcag cagcgtggct taatgaactg actgccagcg ttgaggccat
    21541 gacggcagcg gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg
    21601 atagagagtt tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt
    21661 catggattag cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa acaatgaata
    21721 aaatcctgtt agttgatgat gaccgagagc tgacttccct attaaaggag ttgctcgaga
    21781 tggaaggctt caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg
    21841 acagcattga tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat
    21901 taaaagcact tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg
    21961 aacttgatcg cgttctcggc cttgagctgg gcgcagatga ctatctcccg aaaccattta
    22021 atgatcgtga gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac tggagcgagc
    22081 aacagcaaaa caacgacaac ggttcaccga cactggaagt tgatgcctta gtgctgaatc
    22141 caggccgtca ggaagccagc ttcgacgggc aaacgctgga gttaaccggc actgagttta
    22201 ccctgctcta tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc
    22261 aggaagtgct gggcaaacgc ctgacgcctt tcgaccgcgc tatcgatatg cacatttcca
    22321 acctgcgtcg taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg
    22381 gtcgcggcta tctgatggtt tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc
    22441 catcttctgg ctgacgctgg cgctggtgtt gatgttggtt ttgatgttac ccaagctcga
    22501 ttcacgccag atgaccgagc ttctggatag cgaacagcgt caggggctga tgattgagca
    22561 gcatgttgaa gcggaactgg cgaacgatcc gcccaacgat ttaatgtggt ggcgacgtct
    22621 gttccgggcg attgataagt gggcaccgcc aggacagcgt ttgttattgg tgaccaccga
    22681 aggccgcgtg atcggcgctg aacgcagcga aatgcagatc attcgtaact ttattggtca
    22741 ggccgataac gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc
    22801 gttctccgtg cgtgatggcg aagataatta ccaactttat ctgattcgtc cggccagcag
    22861 ttctcaatcc gatttcatta acttactgtt tgaccgcccg ctattactgc tgattgtcac
    22921 catgttggtc agtacgccgc tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg
    22981 taagctgaaa aacgctgccg atgaagttgc ccagggaaac ttacgccagc acccggaact
    23041 tgaagcgggg ccacaggaat tccttgccgc tggtgccagt tttaaccaga tggtcaccgc
    23101 gctggagcgc atgatgacct ctcagcagcg tctgctttct gatatctctc acgagctgcg
    23161 caccccgctg acgcgtctgc aactgggtac ggcgttactg cgccgtcgta gtggtgaaag
    23221 caaggaactg gagcgtattg aaaccgaagc gcaacgtctg gacagcatga tcaacgatct
    23281 gttggtgatg tcccgtaatc agcaaaaaaa cgcgctggtt agcgaaacca tcaaagccaa
    23341 ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa gccgagcaga tgggcaagtc
    23401 gttgacggtt aacttcccgc ctgggccgtg gccgctgtac ggcaatccga acgccctgga
    23461 aagtgcgctg gaaaatattg ttcgtaatgc tctgcgttat tcccatacga agattgaagt
    23521 gggctttgcg gtagataaag acggtatcac cattacggtg gacgacgatg gtcctggcgt
    23581 tagcccggaa gatcgcgaac agattttccg tccgttctat cggaccgatg aagcacgcga
    23641 tcgtgaatct ggcggtacag gattggggct ggcgattgtt gaaaccgcca ttcagcagca
    23701 tcgtggctgg gtgaaggcag aagacagccc gctgggcggt ttacggctgg tgatttggtt
    23761 gccgctgtat aagcggagtt aaactccgca tttgtaggca ggataaggcg tttacgccgc
    23821 atccggcatt tgagcaggat gcctgatgcg acgctgatag cgtcttatca ggcctacact
    23881 ccgggggtta ttttccccac aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc
    23941 gcttttgcat cgtccgcgtc cagcttttcg ataacccggc ggcggagagt aaacggtgat
    24001 attgatcgtc atcaaacggc atatgccatg cgatggcggc agcctcctgc acggtcacat
    24061 cgctgacacg ggaaaccaac tccagcggcg cgtccgcaga cactttccct ggcgctatca
    24121 cgctatacaa ccaccccacc ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat
    24181 caaaatggta attgagttta tagcaaggcg agcgcggctg gctgacctga attaatgcct
    24241 ctccccagcg gaaaatatcg cccatataaa cattactttc cgtcaggccg tcggttgaga
    24301 ggttttcacc aaacgcaggc gcaacaaaca actccgcctg ttccggaaat tcccgcgccc
    24361 agtagagata atgctcacga ggataatgac acagcgctct gtctggcccg ccgtgaacct
    24421 ttttctccgc ctgctcgtca ccttccagcc ccagctccgt cagcatcaac tcaccatcaa
    24481 cctggatttt ggcaattgcg ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt
    24541 atacatcaac cggatatcgc atctgttccc tctccacgag aaatttcccc attagaccat
    24601 atttatttta tgtcaggcgt gctgttttcg cggtaagtat ccaggcagga actcaatagc
    24661 aaaccggacg aacgttagtt catccggctg tggggattaa ggaagcaggt ttatttcacc
    24721 gtacctaaaa tttcgatttt cgctgctctg gctttgactt tacgtgacca gatagaggta
    24781 agaattggca ccagaatcga agtcacaatg accgccgtcg ctaccagcga agttgctgcc
    24841 ggagccatcg gtttaaacgc aggcaccatt tctgcaatca gcacaggtgt cgctaccgcg
    24901 gcccctgcgg aactggaagc ggcaattccg gcagtgccat cgccaccgcc aatcaattta
    24961 tcggcgataa tcaacggaat accggtcacg ataattactg ccacacccaa caggatcccc
    25021 agcaaaccag tctgggcaat cacagtcaaa tcaatggtat tgcccagcgc gaaggcaaag
    25081 aatggaatca gcgtttgcac cgctttgctg aaaaattctc gtaattcagg gtcaaggttc
    25141 cccagggcaa agcccaccag gaacggtaat actgcgccga cgaaaacatg cggttcaaac
    25201 gaggcaatcc ctgcagtgcc cagaataatc atcgtcatga gcggcccgga ctccaacgac
    25261 atcaacacaa atgccccagc ttcttctttt gtgccgtact gctgcatgat ggaagcgtaa
    25321 agtccgccat tggtcatatc catcgccgcc accagcgcca gcgttgaaag tccggcaaag
    25381 aatccaactt caacaccatg ttccggaata atgcgcgagg caattgccgc aaccacccac
    25441 gcgacggcaa tttttgttac caccagcgta ccggatttac gcaataccgt tcccgtcgcg
    25501 cttaatttta ttgacgcccc catgcaaaaa aaccacaccg ccagaatggg caccgtaccg
    25561 gtaatcatcc cgttggtgaa tgatccaaaa tatttccccg cccccggcga gaaggtgtgg
    25621 cacagtgcgc caaggaatag cgggacgagc atcatccccc ccgggatttt ctcaatcgag
    25681 cgttttatct gcatttccat cacctgtcgg catattttta agggctgatg cgtcattcct
    25741 caatggagca tcagtgttga tggaaacgaa gatagacatt catttaatca ataaaagtga
    25801 tcaaaaccac atcaattgaa acactgtttt atttttatcg gatcattgtt tttattttga
    25861 aattgatcac aaaaaaacca ccgccgttgg cgatggttca ttttttaagc tgatatgcgg
    25921 cctatacgcc tcattgcagc aggcggcaaa tgattatttt ttcgccgcga aacgcgccgc
    25981 agcttcgtcc cagttcacca cgttccagaa ctctttgatg tagtccgggc ggcggttctg
    26041 gaatttcagg tagtaagcat gttcccacac atccaggccc agaatcggga agccggaagc
    26101 gccagaaata gcttcaccca tcagcggaga atcctggtta gcagtagaaa ccaccgccag
    26161 tttatcgcct ttcagcacca gccatgccca gccggaacca aagcgggaag ctgccgcttt
    26221 ttcaaattct gctttgaagt tatcaacgga gccgaagtca cgttcgatag ccgctttcag
    26281 gtcaccctgc agggtggtgc cttttttcag acctttccag aacaggctgt ggttagcgtg
    26341 accgccagcg ttgttgcgca gtacggtttt cttgtctgct ggcagctggt ccagtttggt
    26401 gatcagctct tcaaccggca ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc
    26461 gttgtttacg taggtctgat ggtgtttggt gtggtggatt tccatggtct gcttatcgaa
    26521 gtgcggttcc agggcatcgt aagcatacgg cagggatggc agggtatagc tcatattcat
    26581 ctccagtatt gtcgggcggc cgattgttaa tgccgcgtaa gcagttggtt cattatagtt
    26641 aattaaatga tattgaaaat gattatcaat gccgtacttt tcgtaagggt atggttttgc
    26701 aggaaaatgc ccgagatgtg aagcaaatca cccacttaat gccgtgattg ccagtaaatc
    26761 gacaacggcg gcaacaggcg aaaggttaat cgacagcacg atttttacac tcatctcgtc
    26821 ggagatgtga agcgacgaaa aatgatgagg ataagaagat gagtaacgcg attacgatgg
    26881 ggatattttg gcatttgatc ggcgcggcca gtgcagcctg tttttacgct ccgttcaaaa
    26941 aagtaaaaaa atggtcatgg gaaaccatgt ggtcagtcgg tgggattgtt tcgtggatta
    27001 ttctgccgtg ggccatcagc gccctgttac taccggattt ctgggcgtat tacagctcgt
    27061 ttagtctctc tacgctgctg cctgtttttc tgttcggcgc tatgtggggg atcggtaata
    27121 tcaactatgg cctgaccatg cgttatctcg gcctgtcgat gggaattggc atcgccattg
    27181 gcattacgtt gattgtcggt acgctgatga cgccaattat caacggcaat ttcgatgtgc
    27241 tgattaacac cgaaggcgga cgcatgacgt tgctcggcgt tctggtggcg ctgattggcg
    27301 tagggattgt gactcgcgcc gggcagttga aagagcgcaa aatgggcatt aaagccgaag
    27361 agttcaacct gaaaaaaggg ctggtgctgg cggtgatgtg cggcattttc tctgccggta
    27421 tgtcttttgc gatgaacgcc gcaaaaccga tgcatgaagc cgctgccgca ctgggcgtcg
    27481 atccactgta tgtcgctctg ccaagctatg tcatcatcat gggcggcggc gcgatcatca
    27541 acctcggttt ctgcttcatt cgtctggcaa aagtgaagga tttgtcgcta aaagccgact
    27601 tctcgctggc aaaaccgctg atcattcaca atgtgttact ctcagcactg ggcgggttga
    27661 tgtggtatct gcaattcttt ttctatgcct ggggccacgc ccgcattccg gcgcagtatg
    27721 actacatcag ttggatgctg catatgagtt tctatgtatt gtgcggcggt atcgtcgggc
    27781 tggtgctgaa agagtggaac aatgcaggtc gccgtccggt aacggtgttg agcctcggtt
    27841 gtgtggtgat tattgtcgcc gccaacatcg tcggcatcgg catggcgaat taatctttct
    27901 gcgaattgag atgacgccac tggctgggcg tcatcccggt ttcccgggta aacaccaccg
    27961 aaaaatagtt actatcttca aagccacatt cggtcgaaat atcactgatt aacaggcggc
    28021 tatgctggag aagatattgc gcatgacaca ctctgacctg ccgcagatat tgattgatgg
    28081 tcattccagt ctgctggcga aattgctgac gcaaaacgcg ctcactgcac gatgcctcat
    28141 cacaaaattt atccagcgca aagggacttt tcaggctagc cgccagccgg gtaatcagct
    28201 tatccagcaa cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa cgatggcgat
    28261 tcagcaacat caccaactgc ccgaacagca actcagccat ttcgttagca aacgacacat
    28321 gctgactact ttcatgctca agctgaccga taacctgccg cgcctgcgcc atccccacgc
    28381 tacctaagcg ccagtgtggt tgccctgcgc tggcgctaaa tcccggaatc gctccctgcc
    28441 agtcaagatt cagcttcagc cgctccgggc aataaataat attctgcaaa accagatcgt
    28501 taacggaagc gtaggagtgt ttatcgtcag catgaatgta aaagagatcg ccacgggtaa
    28561 tgcgataagg gcgatcgttg agtacatgca gaccattacc gcgccagaca atcaccagct
    28621 cacaaaaatc atgtgtatgt tcggcaaaaa catcttgcgg ataacggtca gccacagcga
    28681 ctgcctgctg gtcgctggca aaaaaatcat ctttgagaag ttttaactga tgcgccaccg
    28741 tggctacctc ggccagagaa cgaagttgat tattcgcaat atggcataca aatacgttga
    28801 gaagattcgc gttattgcag aaaaccatcc cgtccctgac gaatatcacg cggtgaccag
    28861 ttaaactctc ggcgaaaaag cgtcgaaaag tggttactgt cgctgaatcc acagcgatag
    28921 gcgatatcag taacgctggc ctcgctgtgg cgtagtagat gtcgggcttt catcagtcgc
    28981 aggcggttca ggtaacgctg aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc
    29041 gtacgcagcg aaagagaaaa ttgctccgcc acggcatccc aattcacctc atcggcaaaa
    29101 tggtcctcca gccaggccag aagcaagttg agacgtgatg cgctgttttc caggttctcc
    29161 tgcaaactgc ttttacgcag caagagcagt aattgcataa acaagatctc gcgactggcg
    29221 gtcgagggta aatcattttc cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc
    29281 tgctgcaata cgctgtggtt aacgcgccag tgggaaggat actgcccatc cagctcttgt
    29341 ggcagcaact gattcagccc ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca
    29401 ttagtcagac acagattatc ggtatgttca tacagatgcc gatcatggtc gcgcacaaaa
    29461 cagaccgtgc caccggtgat ggtatagggc tgcccattaa acacatgaat acccgtgcca
    29521 tgttcgacaa tcacaatttc atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg
    29581 agccggggtt ctatcgccac ggacgcgtta ccagacggaa aaaaatccac actatgtaat
    29641 acggtcatac tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt
    29701 tcttacctta aattttcgac ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc
    29761 gtgaattttc aggaaatgcg gtgagcatca catcaccaca attcagcaaa ttgtgaacat
    29821 catcacgttc atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt
    29881 cgcgaattca ggcgcttttt agactggtcg taatgaaatt cagcaggatc acattatgac
    29941 ctttcgcaat tgtgtcgccg tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg
    30001 ttacgagcgt gaatgccgca gcctgacgct gcgcgaaatc catcgtttta acaatgggct
    30061 gcatagtcag aacggctatg tcacctggga tgtggatagc ctggaaagtg ccattcgcct
    30121 tggattaaac aaggtgtgcg aggaagggat tcgtatcgat agcattggga ttgatacctg
    30181 gggcgtggac tttgtgctgc tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta
    30241 tcgcgatagc cgcaccaatg gcctgatggc gcaggcacaa caacaactcg gcaaacgcga
    30301 tatttatcaa cgtagcggca tccagtttct gcccttcaat acgctttatc agttacgggc
    30361 gctgacggag caacaacctg aacttattcc acacattgct cacgctctgc taatgccgga
    30421 ttattttagt tatcgcctta ccggcaagat gaactgggag tacaccaacg ccacaaccac
    30481 gcaactggtc aatatcaata gcgacgactg ggacgagtcg ctactggcgt ggagcggtgc
    30541 caacaaagcc tggtttggtc gcccgacgca tccgggtaat gtcataggtc actggatttg
    30601 cccgcagggt aatgagattc cggtggtcgc cgttgccagc catgataccg ccagcgcggt
    30661 tatcgcctcg ccgttaaacg gttcacgcgc cgcttatctc tcttctggta cctggtcatt
    30721 gatgggcttc gaaagccaga cgccatttac caatgacacg gcgctggcag ccaacatcac
    30781 caatgaaggc ggggcggaag gtcgctatcg ggtgctgaaa aatattatgg gcttatggct
    30841 gcttcagcgg gtgcttcagg aacggcaaat caacgatctc ccggcgctta tcgccgcgac
    30901 acaggcactt ccggcctgtc gcttcatcat caatcccaat gacgatcgct ttattaatcc
    30961 tgaagcgatg tgcagcgaaa ttcaggctgc gtgtcgggaa acggcgcaac cgatcccgga
    31021 aagtgatgct gaactggcgc gctgtatttt cgacagtctg gcgctgctgt atgccgatgt
    31081 gttgcatgag ctggcgcagc tgcgcggtga agatttctcg caactgcata ttgtcggcgg
    31141 cggctgccag aacacgctgc tcaaccagct atgcgccgat gcctgcggta ttcgggtgat
    31201 cgccgggcct gttgaagcct caacgctcgg caatatcggc atccagttaa tgacgctgga
    31261 tgaactcaac aatgtggatg atttccgtca ggtcgtcagc accaccgcga atctgaccac
    31321 ctttacccct aatcctgaca gtgaaattgc ccactatgtg gcgcagattc actctacacg
    31381 acagacaaag gagctttgcg catgaccact caactggaac aggcctggga gctggcgaaa
    31441 cagcgtttcg cggcggtggg gattgatgtc gaggaggcgc tgcgccaact tgatcgttta
    31501 cccgtttcaa tgcactgctg gcagggcgat gatgtttccg gttttgaaaa cccggaaggt
    31561 tcgctgaccg gtgggattca agctactggt aattatccgg gcaaagcgcg taatgccagt
    31621 gagctacgta ccgatctgga acaggctatg cggctgattc cggggccgaa acggcttaat
    31681 ttacatgcca tctatctgga atcagatacg ccagtctcgc gcgatcagat caaaccagag
    31741 cacttcaaaa actgggttga atgggcgaaa gccaatcagc tcggtctgga ttttaacccc
    31801 tcctgctttt cgcatccgct aagcgccgat ggctttacgc tttcccatgc cgacgacagc
    31861 attcgccagt tctggattga tcactgcaaa gccagccgtc gcgtttcggc ctattttggc
    31921 gagcaactcg gcacaccatc ggtgatgaac atctggatcc cggatggtat gaaagatatc
    31981 accgttgacc gtctcgcccc gcgtcagcgt ctgctggcag cactggatga ggtgatcagc
    32041 gagaagctaa accctgcgca ccatatcgac gccgttgaga gcaaattgtt tggcattggc
    32101 gcagagagct acacggttgg ctccaatgag ttttacatgg ggtatgccac cagccgccag
    32161 actgcgctgt gcctggacgc cgggcacttc cacccgactg aagtgatttc cgacaagatt
    32221 tccgccgcca tgctgtatgt gccgcagttg ctgctgcacg tcagccgtcc ggttcgctgg
    32281 gacagcgatc acgtagtgct gctggatgat gaaacccagg caattgccag tgagattgtg
    32341 cgtcacgatc tgtttgaccg ggtgcatatc ggccttgact tcttcgatgc ctctatcaac
    32401 cgcattgccg cgtgggtcat tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg
    32461 ctggaaccta ccgctgagct gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg
    32521 gcactgctgg aagagcagaa atcgttgccg tggcaggcgg tctgggaaat gtattgccaa
    32581 cgtcacgata cgccagcagg tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg
    32641 attttgagcc agcgtgggta aacactgccg gatgcggcgc gagcgcctta tccggcctac
    32701 aggtcgaatg tccatgcggg ctgtcaccct caccccggcc ctctccctga aagggcgagg
    32761 gagaaaaaca tagcctcgtg cggcaacgga gctgaacact gctccggaca gtcccctcgc
    32821 ccctacgggg agagggttag ggtgaaggga acaagcagca ccatgtctaa acaaggcaac
    32881 ctaatttaca ggaacacaga acatgcaaaa cattacccag tcctggtttg tccagggaat
    32941 gatcaaagcc accaccgatg cctggctgaa aggctgggac gagcgcaacg gcggtaacct
    33001 gacgctgcgc ctggatgacg ccgatatcgc gccttataaa gacaacttcc atgcacaacc
    33061 gcgctatatc ccgctcagcc aacccatgcc tttactggca aatacaccgt ttattgtcac
    33121 cggctccggc aaattcttcc gtaacgtcca gcttgatcct gcagctaact taggcgtcgt
    33181 aaaagtcgac agcgacggcg cgggctacca tattctctgg gggttaacca acgaagccgt
    33241 tccaacttct gaacttccgg ctcactttct ctcccactgc gagcgcatta aagccaccaa
    33301 cggtaaagat cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt
    33361 actggaaaac gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgagtgtct
    33421 ggtagtgttc ccggatggcg ttggcatttt gccgtggatg gtgcccggca cggacgaaat
    33481 cggccaggcg accgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg
    33541 cgtcttcggc agcgggtcga cgttggatga aacattcggt ttaatcgaca ccgcagaaaa
    33601 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
    33661 tgaagagttg atagcgctcg gcaagcgctt cggcgttaca ccactagcta gtgcgctggc
    33721 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca
    33781 cgaagagtat cagcgtcggc ataaccccat ctggccggaa ctggaagcag tgttgaaatc
    33841 tcacggtgcg cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccac
    33901 ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agcactgatg tttgccaacg
    33961 ttggtggaaa tatatgaccg atgttatgcc cgctaacccg gataacagcc cggtgagtag
    34021 cgagctgcaa gaagtgtttt acctgccgta attgcccaat gtgccagaga tgcctccggc
    34081 acaatatttt aaatttcgat ttgaaaaatt aaacatatta catctcccgg taagttatat
    34141 ttccctgata cattgtgagt aaatcacaaa aataatgaat aacccattaa tgattcatgt
    34201 ggtttattta aataaccatt atgtgcatta ctccgcaaat ctgacctttc actctgtctc
    34261 aaattgtcat tgtttaccga catatcgcct ctcatcactt aatcgctcca ccattacaat
    34321 tgattaaaat atattttatg gagtccgatt aatggcagtt cttactgcaa gctgtattga
    34381 cctgaatatt cagggcaatg gcgcttattc cgttctgaag cagttggcga caatagcgtt
    34441 acaaaacggt tttatcaccg actcacacca gttcctgcaa accctgctgc tgcgcgaaaa
    34501 aatgcactct actggatttg gttccggtgt cgccgtgccg cacggtaaaa gcgcctgcgt
    34561 taaacaaccg ttcgtattat tcgcccgcaa agcgcaggct attgactgga aagccagcga
    34621 tggcgaagac gtcaattgct ggatctgcct cggcgtgccg caaagcggcg aagaggatca
    34681 ggtcaaaatc atcggcacac tgtgtcgcaa aattattcac aaggaattta ttcatcaact
    34741 gcaacagggc gataccgacc aggtgcttgc cttgttaaat caaaccctca gctcataagg
    34801 aagtggcgat ggagtcatcc ttacgtattg tcgcgatcac caactgcccc gccgggatcg
    34861 ctcacaccta catggtggcg gaagccctgg aacagaaagc ccgttctctc ggtcatacca
    34921 ttaaagttga aactcaagga tcaagcggcg tcgaaaaccg cttatccagc gaagagattg
    34981 ccgctgccga ttacgtcatt ctcgctaccg ggcgtggcct gagcggtgac gatcgcgcgc
    35041 ggtttgccgg gaagaaggtt tatgagattg ccatctccca ggcgctgaaa aatatcgatc
    35101 agattttcag tgaattacca acaaactcgc atctttttgc tgcagatagc ggcgtgaagc
    35161 ttggcaaaca ggaagtgcaa agcggcagcg tgatgagcca cctgatggcg ggcgtctctg
    35221 cggcgctgcc gtttgtcatc ggcggcggta ttctggtggc gcttgccaac atgctggtgc
    35281 agttcggttt gccatatacc gatatgtcga aaggtgcgcc gtcgtttacc tgggtagttg
    35341 aatccatcgg ttacctcggc ttcaccttta tgatccccat catgggcgct tatattgcct
    35401 cgtcgattgc cgataaaccg gctttcgctc cggcgtttct ggtttgctat ctggcgaacg
    35461 acaaagcgct gctcggcacc cagtcgggtg cgggattcct cggcgcggtg gtgctggggc
    35521 tggcgattgg ctatttcgtc ttctggttcc gcaaagtgcg ccttggcaaa gcattacaac
    35581 cgctgctcgg ttcaatgctg atcccgttcg ttactctgct ggttttcggc gtgctcactt
    35641 actacgttat cgggccggtg atgtccgacc tcatgggcgg gctgctccac ttcctgaaca
    35701 ccattcctcc atcaatgaag tttgcggcag cgtttctggt cggtgcgatg ctggcgttcg
    35761 atatgggcgg ccccatcaat aaaaccgcct ggttcttctg cttctcactg ctggaaaaac
    35821 acatttatga ctggtacgcc atcgtcggcg ttgtcgcgtt gatgccgccc gtcgcggcgg
    35881 gtcttgcgac gttcattgcg cccaaactgt ttacccgaca ggaaaaagag gctgccagta
    35941 gtgccattgt ggtcggtgcc acagtcgcaa ccgaaccggc tattccttac gctctggcag
    36001 ctccgttgcc gatgattacc gccaatacgc tggcgggcgg cattaccggc gtgctggtga
    36061 tcgctttcgg aatcaaacgt ctggcgcctg gtttgggtat ctttgacccg ctgatcggcc
    36121 tgatgtcgcc ggtaggttcg ttttatctgg tgctggcgat tggcctcgcg ctgaacattt
    36181 cattcattat cgtcctgaaa ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg
    36241 agcttgtcca tgaacattga gttactgcaa cagttgtgcg aagccagcgc cgtcagcggc
    36301 gatgaacagg aagttcgcga catcctgata aacacgctgg aaccttgcgt taatgaaatc
    36361 acctttgatg gtctgggcag ctttgttgcc cgtaagggga ataaaggtcc aaaagttgcc
    36421 gttgtcgggc atatggatga agtcggcttt atggtcaccc acatcgacga gagcggtttt
    36481 ctgcgtttta ccaccattgg cggctggtgg aatcagtcga tgctcaacca ccgggtaacc
    36541 atacgcacac acaagggagt gaaaatccct ggtgtgattg gttccgtcgc gcctcatgcg
    36601 ttaacggaaa agcaaaagca acaaccgctg tcatttgatg agatgttcat tgatattggc
    36661 gcgaacagtc gcgatgaggt ggaaaaacgc ggtgtggcga tgggcgattt tattagcccg
    36721 gaagccaatt ttgcctgctg gggcgaagat aaagtggtcg gcaaggcgct ggataaccgc
    36781 atcggctgcg caatgatggc tgaactattg cagacggtga ataatcccga aattacgctg
    36841 tatggcgtcg gcagcgtgga agaagaagtt gggctacgcg gggcacaaac ctcggcggaa
    36901 cacattaaac cggatgtagt gatcgtgctg gataccgctg tcgcaggcga tgttccgggc
    36961 attgataaca ttaaataccc gctgaaactg ggccaggggc cggggctgat gctgtttgac
    37021 aagcgctact tccccaacca gaaactggta gcagcgttaa aaagctgtgc cgcacataac
    37081 gatttaccgc tgcaattttc caccatgaaa accggtgcga cggatggcgg gcgctacaac
    37141 gtgatgggcg gcgggcgtcc ggttgttgcg ctgtgcctgc caacccgcta tctgcacgct
    37201 aacagcggta tgatttcaaa agccgattac gatgctctgc tcacgttgat acgggatttt
    37261 ctgaccacct taaccgcgga gaaagtcaac gcgtttagcc agttccgtca ggtggattaa
    37321 tgctaaacga acgccagtta aagattgtcg atctgctgga gcaacagccg cgcacgcctg
    37381 gtgagctggc gcaacagact ggcgtttcag gcaggaccat cctgcgtgat attgactatc
    37441 tcaactttac ccttaacggc aaagcccgca tttccgccag tggtagtgcg ggctatcagc
    37501 tggaaatctt cgagcgccgc agcttttttc agttgctgca aaagcacgat aacgacgatc
    37561 ggctgctggc actgttatta ctgaatacct ttactccccg tgcgcaactc gcctcggcgc
    37621 ttaatttgcc agaaacgtgg gtcgcagagc gtctgccccg gttaaaacag cgttatgaac
    37681 gcacttgttg cctggccagc cgccctggtt tgggccattt cattgatgag acagaagaga
    37741 aacgcgttat cttgctggcg aacttgctgc gcaaagatcc gtttttaatt ccgctggcgg
    37801 gcgtaacacg agacaacctt cagcatttat ccacggcctg cgacaaccaa caccgctggc
    37861 cgctcatgca gggtgattat ctctccagcc tgattctggc gatttacgcc ctgcgtaatc
    37921 aactgaccga tgagtggccg caatatcccg gtgacgagat aaaacaaatc gttgaacata
    37981 gcggtctgtt tcttggtgat aacgctgtaa gaaccctgac aggtttgata gagaaacagc
    38041 atcagcaagc gcagataatt tctgccgata atgtgcagcg gttgctgcaa agggtgccgg
    38101 gcatcgcgtc attgaatatt attgatacgc agctggttga gaacattacc gggcatttat
    38161 tacgttgcct tgccgcaccg gtgtggattg ctgagcaccg ccaaagcagc atgaataacc
    38221 tgaaagccgc ctggcctgcg gcctttgata tgagtctgca ctttattacg ctactgcgtg
    38281 aacagctcga tattcccctt ttcgacagcg atctgatcgg tttgtatttt gcctgtgcgc
    38341 tggaacggca tcaaaacgaa cgccagccga tcattttgct ctcggaccag aacgcgattg
    38401 ccactattaa tcagctcgcc attgagcgcg atgttttaca ttgtcgggtg attattgccc
    38461 gtagcttaag cgaacttgtt gccattcgcg aagagattga gccgttattg atcattaaca
    38521 acagccatta tttactggat gacgcggtaa ataattacat caccgtaaaa aatatcatta
    38581 cggctgccgg tatcgaacaa ataaaacatt ttctggcgac ggcatttatt cgccaacagc
    38641 cggagcgttt tttctctgcc cccggaagtt ttcattattc gaatgtacgc ggtgaaagct
    38701 ggcaacatat tacccggcaa atttgtgcgc aattagtggc acaacaccat attaccgccg
    38761 atgaagcaca acgcatcatc gcccgcgaag gcgaaggtga aaacctgatt gttaatcgcc
    38821 tcgccatccc acattgctgg agcgaacagg agcgacgttt tcgtggattt tttattaccc
    38881 tcgcccaacc agttgaggtg aataacgaag tcattaacca tgtcttgatc gcctgcgccg
    38941 ccgccgatgc gcgtcatgag ctaaaaatat ttagctatct ggcaagcgta ttgtgtcagc
    39001 atccggcaga ggttattgcc gggttaacag gatatgaggc atttatggag ttacttcaca
    39061 aggggtgaac tgtgtcggat gcgccgcatc cgacactgca tttatcatca aaaacgttta
    39121 acgcaggcta ttatagtcat caaccatttc gttgtactcg cgtaatgctc tcgggaaaga
    39181 gtcttcgacc atccagcttg agtttgcgtc ctgaagttgc tctttatccc agtcagagta
    39241 cgggacttta tctctgatgc ggcgaacgta ttttttagcc tcgagttgat actggcctgc
    39301 cgaattaata aacgagaaat tcatgccgcc tttatccgct tctttggcct gcgccactag
    39361 cgtttccagt tcagacactt tcttcattgc cgcatcaaca tcaaacttct cttgcgatat
    39421 caggttatta atttgtttcg ctgaaatcat gactgccaga gagtagtagt ggaatgtttt
    39481 cccttctctt tcttcaatat ttttcagttc cgcaagctgg cgcttatcgt taatttcctg
    39541 aatcgccgca tgataagatt ctgctacagg ttcaaaggct tccagatttt ttaagaatgt
    39601 ctggtgcagc gttttccctt tcgcaaacgc atcgtcttta taattttcct gggtgtaata
    39661 tttatccatt tcgttaatgg tattacccaa cgccacggca gcatcgatat aactcaccgc
    39721 cacgccatca attggttgca gcgcaggcgt taacgccaca gcacttttta cgcctttttc
    39781 acactcagcg aggttggatt cactaatgcc gtagatgcca taaacagtac gctcttcacc
    39841 ggtaggcccc tgtttaaaat ctttcagcca gtcagcataa cgcgccagac tgctctgtac
    39901 agggatctgt aacttgttat agcacttgat atagacatcc attttttcgt cgaaaaccgc
    39961 ttgttcatcg accaccggtt gcgcaggtgt ttcaggttta gcttctgctt ttgcaacggg
    40021 agcttcagga gctggtgcgg caggttgact attggcaggc gggggcgttt ctgtttcggc
    40081 ttttttatca tcacaacccg tcagaccgag ggccatgatg gcgactataa tagcggaaga
    40141 taataaattc cttttcattt aattgcctta taaatatata gataagagag tgaacataat
    40201 aaatgaaaaa agaaaacacc actatacgcg ttttatttat aaataaaata ataaaattca
    40261 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
    40321 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
    40381 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
    40441 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
    40501 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
    40561 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
    40621 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
    40681 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
    40741 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
    40801 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
    40861 tcaaggccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
    40921 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
    40981 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
    41041 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
    41101 atatttgtca cattttcgat ttctttttgc tgtgtttttt tcactttctc ttaaccagat
    41161 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctgga tggagggaaa
    41221 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
    41281 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
    41341 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
    41401 gcggcactgg gttttgcacc cagcgtagcg ctcgcggaaa cccggcagta taaactgctg
    41461 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
    41521 tacagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
    41581 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
    41641 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
    41701 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
    41761 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
    41821 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
    41881 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
    41941 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
    42001 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
    42061 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
    42121 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
    42181 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccgcgaatac
    42241 accgaagcct ataccaacgc cagcctgata gtgcgtgagg attacggctt tgaagatggc
    42301 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
    42361 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
    42421 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
    42481 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
    42541 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
    42601 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
    42661 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctgctgtcg
    42721 cagagcctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
    42781 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
    42841 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
    42901 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
    42961 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
    43021 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
    43081 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
    43141 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
    43201 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
    43261 gccccgggga ttgcgctgac tgatggcgag atcctctccg gtatcttcct gcgcttgcgc
    43321 aagatgtatg ccgaacaggg tggcgcgaac ccggaccagg tgctgaacat gacctggaac
    43381 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
    43441 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
    43501 agctcgttcg cccaattgcg cgatgacggt acaacctcct gtggctgctg gattttcgcc
    43561 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
    43621 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
    43681 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
    43741 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
    43801 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
    43861 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
    43921 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
    43981 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
    44041 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcaaccaga gcagtttgtg
    44101 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
    44161 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgtacgctg
    44221 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ttatgaaggc
    44281 gtagctaaaa aaggctttat tgccaatacg ttaacgccat tcgttggtga tgcgaacacg
    44341 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
    44401 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
    44461 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgtcac cacctgtatc
    44521 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
    44581 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
    44641 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
    44701 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
    44761 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
    44821 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
    44881 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
    44941 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
    45001 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
    45061 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
    45121 ccggagaacc tggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
    45181 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
    45241 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
    45301 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
    45361 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
    45421 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
    45481 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat cacaacctaa
    45541 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
    45601 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
    45661 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
    45721 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
    45781 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
    45841 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
    45901 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
    45961 gctgggttcg agcgagaaac gtacggcgga tatgattccg ccgttattgt tccctcggct
    46021 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
    46081 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
    46141 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
    46201 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
    46261 gattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
    46321 gaaggcatcg actcaggagc tggaagatat ggccagcgca ctgtttgcct ctgatttctc
    46381 atccgtcagc agcgataaag cgccgtttat ctgggctgca ctgtcgctct actgggcgca
    46441 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgagcaac gtcaatattg
    46501 cccggtttgt ggttctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
    46561 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
    46621 caactgtgaa cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
    46681 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
    46741 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
    46801 gcaagaaggc tacgcccgca gttccatcaa cccgttcctg ttcccgggtg aaggggagta
    46861 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagcccatag ggtgtgacga
    46921 caccatctcc cgtattccgc gttaccacct caagctatgc cgccagtgcc tgtttgcctc
    46981 gttcttccct ccaacacatc gataaggtta ttggtatgtc actgatgaca agcgtaa
//
LOCUS       DAHBHC010000032        46770 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHBHC010000032 DAHBHC010000000
VERSION     DAHBHC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.032"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="M2A35_002231"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="M2A35_002231"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCB7198528.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="M2A35_002232"
     CDS             complement(804..1352)
                     /locus_tag="M2A35_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCB7198529.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="M2A35_002233"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="M2A35_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCB7198530.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="M2A35_002234"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="M2A35_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCB7198531.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     HSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRAPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="M2A35_002235"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="M2A35_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCB7198532.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDENEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="M2A35_002236"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="M2A35_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCB7198533.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="M2A35_002237"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="M2A35_002237"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCB7198534.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="M2A35_002238"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="M2A35_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCB7198535.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="M2A35_002239"
     CDS             complement(11782..12675)
                     /locus_tag="M2A35_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCB7198536.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="M2A35_002240"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="M2A35_002240"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCB7198537.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAAPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="M2A35_002241"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="M2A35_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCB7198538.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /locus_tag="M2A35_002242"
                     /pseudo
     CDS             complement(13806..15038)
                     /locus_tag="M2A35_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="M2A35_002243"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="M2A35_002243"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCB7198539.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYSGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMQLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="M2A35_002244"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="M2A35_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCB7198540.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFDHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="M2A35_002245"
     CDS             complement(17843..20107)
                     /locus_tag="M2A35_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCB7198541.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTIFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITTTDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAIHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGVRLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGMNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="M2A35_002246"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="M2A35_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCB7198542.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="M2A35_002247"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="M2A35_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCB7198543.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="M2A35_002248"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="M2A35_002248"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCB7198544.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="M2A35_002249"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="M2A35_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCB7198545.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="M2A35_002250"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="M2A35_002250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCB7198546.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="M2A35_002251"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="M2A35_002251"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCB7198547.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="M2A35_002252"
     CDS             complement(26972..27664)
                     /locus_tag="M2A35_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCB7198548.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /locus_tag="M2A35_002253"
     CDS             27794..29554
                     /locus_tag="M2A35_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HCB7198549.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYENLQRAKAAFWAK"
     gene            29960..30817
                     /gene="focA"
                     /locus_tag="M2A35_002254"
     CDS             29960..30817
                     /gene="focA"
                     /locus_tag="M2A35_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCB7198550.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            30872..33154
                     /gene="pflB"
                     /locus_tag="M2A35_002255"
     CDS             30872..33154
                     /gene="pflB"
                     /locus_tag="M2A35_002255"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCB7198551.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQIEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            33346..34086
                     /gene="pflA"
                     /locus_tag="M2A35_002256"
     CDS             33346..34086
                     /gene="pflA"
                     /locus_tag="M2A35_002256"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCB7198552.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34168..34758)
                     /locus_tag="M2A35_002257"
     CDS             complement(34168..34758)
                     /locus_tag="M2A35_002257"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCB7198553.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            34858..35766
                     /locus_tag="M2A35_002258"
     CDS             34858..35766
                     /locus_tag="M2A35_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7198554.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(35767..37197)
                     /locus_tag="M2A35_002259"
     CDS             complement(35767..37197)
                     /locus_tag="M2A35_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCB7198555.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(37407..38555)
                     /locus_tag="M2A35_002260"
     CDS             complement(37407..38555)
                     /locus_tag="M2A35_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7198556.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGADGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            38870..39496
                     /locus_tag="M2A35_002261"
     CDS             38870..39496
                     /locus_tag="M2A35_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCB7198557.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(39532..40395)
                     /gene="dmsC"
                     /locus_tag="M2A35_002262"
     CDS             complement(39532..40395)
                     /gene="dmsC"
                     /locus_tag="M2A35_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCB7198558.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(40397..41014)
                     /gene="dmsB"
                     /locus_tag="M2A35_002263"
     CDS             complement(40397..41014)
                     /gene="dmsB"
                     /locus_tag="M2A35_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCB7198559.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(41025..43469)
                     /gene="dmsA"
                     /locus_tag="M2A35_002264"
     CDS             complement(41025..43469)
                     /gene="dmsA"
                     /locus_tag="M2A35_002264"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCB7198560.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(43769..44761)
                     /locus_tag="M2A35_002265"
     CDS             complement(43769..44761)
                     /locus_tag="M2A35_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7198561.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDARRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAINFNPLAESVHKLSID"
     gene            complement(44831..45172)
                     /locus_tag="M2A35_002266"
     CDS             complement(44831..45172)
                     /locus_tag="M2A35_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7198562.1"
                     /translation="MVNMSRDYAKKLKAIRNAEKLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            45276..45797
                     /locus_tag="M2A35_002267"
     CDS             45276..45797
                     /locus_tag="M2A35_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7198563.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDALADELPELEPPEWHNWKSYWTYDRQKRKFFPANKEDCQTINGKRVYTGR
                     SSKLERLKSSEGN"
     gene            45808..46014
                     /locus_tag="M2A35_002268"
     CDS             45808..46014
                     /locus_tag="M2A35_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198564.1"
                     /translation="MTSNIISAILNLVFLEVGVIAIYLLNKLTGQKERFIVLNVHYLA
                     AYTRGLFPTTIGAVLIAFVIWLIG"
     gene            46150..46500
                     /locus_tag="M2A35_002269"
     CDS             46150..46500
                     /locus_tag="M2A35_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCB7198565.1"
                     /translation="MKQQRNSSQQRFRNGAERHANRFTTSASRSNSRYSLSETHATPD
                     GHTVKQIGEHTWLIEKAGIVVHRCPRNPFTGNRIFALNCGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            46511..>46770
                     /locus_tag="M2A35_002270"
     CDS             46511..>46770
                     /locus_tag="M2A35_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198566.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDAVVPAEKDVATYLYLN
                     GTRLFVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEA"
BASE COUNT        11810 a        12418 c        11158 g        11384 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgta acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcgc gaacggcagg cgcaggttta
     2461 ctacgagatg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtccagca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccagccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgcgcg ctgccccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccagcg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttcaga cgtacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcgcggga ggttaactcc tcggtcagtc
     4441 ggctaagttc gatttccatc tgctcgatgg cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgtt
     4561 ccggacgttt tggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gtgcaaatca tcagcggaga
     4681 gataagccag ctcgcgacgg tgtaagcgcc gttcaacgcc gttatctttc accatgcgca
     4741 tcactgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcagat tgtccatctc cgcttcgcag aaagtcagtg
     4861 ctttttccag ctggttgcgg cgggaccggt tattactcag ttgcgcatgc agctcgtcac
     4921 ggcgaattcg cgcccgctct tctgccccgc tatcggcgcg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 taaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatccccttc gatcaaatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acgccgccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcagca
     6601 gttcgcgggc gacatcccaa gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgca cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtagc tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgcgtcggca gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atagtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcgttt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc ggaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt ttcgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg actgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagccc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacagc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc taaatctggc ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaaac gctgggcaaa gacgcggttt ttatccatat
     9241 cgatcgcagt acgaataaat ttgtgtacgt ggcgatcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccagctgata atacggtcga gtttgctctg cagatcgaac accagacgat
     9361 cgacaaaatg caggtcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaacttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ctgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 caatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatcag ctgcgcgttt gagttcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtattattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac gggcttggct gccaggtgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggtctt ttgcaaaacg
    13081 gtcacgatcc cacggaatgg tggcgcgtga gaagtacaga gcataccctt cagcgtcgag
    13141 aacgactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcgccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgatggtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tactctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagctgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc actatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgcca cggtgctcaa gtaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt cgtccagaat cagaatcggg ctatcacgca
    16321 acaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccata gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agttcgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gcaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgcgtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacgt tgcggaattc cacgtcacct gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagcgat ttcagcggac
    16921 gcataagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagcttgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcgaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc ctttagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atacaacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa
    17461 aaaaggaaac cggcattccc atcatgtgat caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgagac tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgttac ttgcccctga tgaggagtat cgagccacgt ataaccctgt tgttgatagc
    18001 gatgcttaac tttgttagag ggcagtcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gcctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt catcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtatgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcagg ccaggccagt cccgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacttg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagtaagc
    18661 ctccagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttccg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacgggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc
    19321 caattaatgg tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt agggacggat aaccgtaccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcccgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttgacc atgaatggca cgaactttga gtttcattga ccacagttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca cgaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtgatttga ccgtaatgag tggtcatgcc gtcagtagta gtgatctcaa
    19861 caattgcatc ctgcgtcgca ccagttaagg tttctcctgc ccacaaaatt tgctttgccg
    19921 ctaatatacc ccaaacgaaa aacagcaacg tcagcgcgat ataacgtacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagatag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacgttca gaagaattta acgagagggt gaaaactgct
    20221 caaaactctg cgaagcagct tccagttcca gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct ctcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattactttg aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gcctggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccaggtg ggatgccagc
    23041 ggtaccagcg catcttccga tgcaacatca acatggtgat gtaatgccgc tagtgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt gatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgcccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt atgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agttgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaacct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcattaat aaaataattg taattataag gttaaatatc ggtaatttgt atttaataaa
    29701 tacgatcgat attgttactt tattcgcctg atgctccctt ttaattaact gttttagcgg
    29761 aggatgcgga aaaaattcaa ctcatttgtt aatttttaaa atttattttt atttggataa
    29821 tcaaatattt actccgtatt tgcataaaaa ccatgcgagt tacgggccta taagccaggc
    29881 gagatatgat ctatatcaat ttctcatcta taatgctttg ttagtatctc gtcgccgact
    29941 taataaagag agagttagtg tgaaagctga caaccctttt gatcttttac ttcctgctgc
    30001 aatggccaaa gtggccgaag aggcgggtgt ctataaagca acgaaacatc cgcttaagac
    30061 tttctatctg gcgattaccg ccggtgtttt catctcaatc gcattcgtct tctatatcac
    30121 agcaaccact ggcacaggca caatgccctt cggcatggca aaactggttg gcggcatttg
    30181 cttctctctg gggctgattc tttgtgttgt ctgcggagcc gatctcttta cttccaccgt
    30241 gttgattgtt gttgctaagg cgagtgggcg catcacctgg ggtcagttgg cgaaaaactg
    30301 gctaaatgtc tattttggca acctggtcgg cgcactgctg tttgtacttt taatgtggct
    30361 ttccggcgag tatatgaccg caaatggtca atggggacta aacgtcctac aaaccgccga
    30421 ccacaaagtg caccatactt ttattgaggc cgtctgtctt ggtatcctgg caaacctgat
    30481 ggtatgtctg gcagtatgga tgagttattc tggccgcagc ctgatggaca aagcgttcat
    30541 tatggtgctg ccggtcgcga tgtttgttgc cagcggtttt gagcacagta tcgcaaacat
    30601 gtttatgatc ccgatgggta ttgtaatccg cgacttcgca tccccggaat tttggaccgc
    30661 agtcggttct gcaccggaaa atttttctca cctgaccgtg atgaatttca tcactgataa
    30721 cctgattccg gttacgatcg gcaacattat cggtggtggt ttgttggttg ggttgacata
    30781 ctgggtcatt tacctgcgtg aaaacgacca ccattaatgg ttgtcgaagt acgcagtaaa
    30841 taaaaaatcc acttaagaag gtaggtgtta catgtccgag cttaatgaaa agttagccac
    30901 agcctgggaa ggttttacca aaggtgactg gcagaatgaa gtaaacgtcc gtgacttcat
    30961 tcagaaaaac tacactccgt acgagggtga cgagtccttc ctggctggcg ctactgaagc
    31021 gaccaccacc ctgtgggaca aagtaatgga aggcgttaaa ctggaaaacc gcactcacgc
    31081 gccagttgac tttgacaccg ctgttgcttc caccatcacc tctcacgacg ctggctacat
    31141 caacaagcag attgagaaaa tcgttggtct gcagactgaa gctccgctga aacgtgctct
    31201 tatcccgttc ggtggtatca aaatgatcga aggttcctgc aaagcgtaca accgcgaact
    31261 ggatccgatg atcaaaaaaa tcttcactga ataccgtaaa actcacaacc agggcgtgtt
    31321 cgacgtttac actccggaca tcctgcgttg ccgtaaatct ggtgttctga ccggtctgcc
    31381 agatgcatat ggccgtggcc gtatcatcgg tgactaccgt cgcgttgcgc tgtacggtat
    31441 cgactacctg atgaaagaca aactggcaca gttcacttct ctgcaggctg atctggaaaa
    31501 cggcgtaaac ctggaacaga ctatccgtct gcgcgaagaa atcgctgaac agcaccgcgc
    31561 tctgggtcag atgaaagaaa tggctgcgaa atacggctac gacatctctg gtccggctac
    31621 caacgctcag gaagctatcc agtggactta cttcggctac ctggctgctg ttaagtctca
    31681 gaacggtgct gcaatgtcct tcggtcgtac ctccaccttc ctggatgtgt acatcgaacg
    31741 tgacctgaaa gctggcaaga tcaccgaaca agaagcgcag gaaatggttg accacctggt
    31801 catgaaactg cgtatggttc gcttcctgcg tactccggaa tacgatgaac tgttctctgg
    31861 cgacccgatc tgggcaaccg aatctatcgg tggtatgggc ctcgacggtc gtaccctggt
    31921 taccaaaaac agcttccgtt tcctgaacac cctgtacacc atgggtccgt ctccggaacc
    31981 gaacatgacc attctgtggt ctgaaaaact gccgctgaac ttcaagaaat tcgccgctaa
    32041 agtgtccatc gacacctctt ctctgcagta tgagaacgat gacctgatgc gtccggactt
    32101 caacaacgat gactacgcta ttgcttgctg cgtaagcccg atgatcgttg gtaaacaaat
    32161 gcagttcttc ggtgcgcgtg caaacctggc gaaaaccatg ctgtacgcaa tcaacggcgg
    32221 cgttgacgaa aaactgaaaa tgcaggttgg tccgaagtct gaaccgatca aaggcgatgt
    32281 cctgaactat gatgaagtga tggagcgcat ggatcacttc atggactggc tggctaaaca
    32341 gtacatcact gcactgaaca tcatccacta catgcacgac aagtacagct acgaagcctc
    32401 tctgatggcg ctgcacgacc gtgacgttat ccgcaccatg gcgtgtggta tcgctggcct
    32461 gtccgttgct gctgactccc tgtctgcaat caaatatgcg aaagttaaac cgattcgtga
    32521 cgaagacggt ctggctatcg acttcgaaat cgaaggcgaa tacccgcagt ttggtaacaa
    32581 tgatccgcgt gtagatgacc tggctgttga cctggtagaa cgtttcatga agaaaattca
    32641 gaaactgcac acctaccgtg acgctatccc gactcagtct gttctgacca tcacttctaa
    32701 cgttgtgtat ggtaagaaaa ctggtaacac cccagacggt cgtcgtgctg gcgcgccgtt
    32761 cggaccgggt gctaacccga tgcacggtcg tgaccagaaa ggtgctgtag cgtctctgac
    32821 ttccgttgct aaactgccgt ttgcttacgc taaagatggt atctcctaca ccttctctat
    32881 cgttccgaac gcactgggta aagacgacga agttcgtaag accaacctgg ctggtctgat
    32941 ggatggttac ttccaccacg aagcatccat cgaaggtggt cagcacctga acgttaacgt
    33001 gatgaaccgt gaaatgctgc tcgacgcgat ggaaaacccg gaaaaatatc cgcagctgac
    33061 catccgtgta tctggctacg cagtacgttt caactcgctg actaaagaac agcagcagga
    33121 cgttattact cgtaccttca ctcaatctat gtaattagat ttgactgaaa tcgtacagta
    33181 aaaagcgtac aataaaggct ccacgaaagt ggggcctttt ttagcacgag agcctttttt
    33241 gtcagctatc tatactttaa ggtgactgcc aaaacagact cgacgtagcc ttcgagctgc
    33301 gcaccaacac ggcctcagat gggccacatc tggagaaaca ccgcaatgtc agttattggt
    33361 cgcattcact cctttgaatc ctgtggaacc gtagacggcc caggtattcg ctttatcacc
    33421 tttttccagg gctgcctgat gcgctgcctg tattgtcata accgcgacac ctgggatacg
    33481 catggcggta aagaagttac cgttgaagat ttaatgaaag aggtggtgac ctatcgccac
    33541 tttatgaacg cttccggcgg cggcgttacc gcatccggcg gtgaggcaat cctacaagct
    33601 gagtttgttc gtgactggtt ccgcgcctgc aaaaaagaag gcattcatac ctgtctggac
    33661 accaacggtt ttgttcgtcg ttacgatccg gtgattgatg aactgctgga agtaaccgac
    33721 ctggtaatgc tcgatctcaa acagatgaac gacgagatcc accaaaatct ggttggagtt
    33781 tccaaccacc gcacgctgga gttcgccaaa tatctggcga acaaaaatgt gaaggtgtgg
    33841 atccgctacg ttgttgtccc aggctggtct gacgatgacg attcagcgca tcgcctcggt
    33901 gaatttaccc gtgatatggg caacgttgag aaaatcgaac tcctccccta ccacgagctg
    33961 ggcaaacaca aatgggtggc aatgggtgaa gagtacaaac tcgacggtgt taaaccacct
    34021 aagaaagaga ccatggaacg cgtgaaaggc attcttgagc agtacggtca taaggtaatg
    34081 ttctaatttc cccgtaaagc ggcaacttta ttgagttgcc gcttttttat tccgcgcttc
    34141 attctgctac agcgttttta acacatttta tagtgcatct accatatccc gtacctgaga
    34201 taataacgac tgataatagc tcgcatgaag ttcttgccca tcgaatacca ctgtattaca
    34261 caagaaagtg acatcggcat cttcaatacc aagataactg cacatatttt tcaaataatc
    34321 gcttatattt ttttcccagc ccatctggac aaatgattct ttgtctcctc caaccagcgc
    34381 cacccaacga actttattga atggtaattt gtgcccatct ccataagcca gcccattatt
    34441 ccatactctg tcaatatatc cttttagcat tgctgggaag ctgtaccacc agagaggaaa
    34501 aaccaccact aacgtgtcat gttcaagcag ctctgaataa agctgatgaa cctctggaga
    34561 ataacgttta tccatattct tccagtctgg ttcatcttcc gacgtcatca ccgggttgaa
    34621 attacgccga tataaatcga gttccgtcac ctgtattttc cgctccattg cccgctgatg
    34681 gatcgcatca gcaatatgtg cggtcaatga atcatgacga ggatgggccc ataccaaata
    34741 aatacgttca gactgcataa ttttacctcc acgttaagtt ctggagcgca ctttacgcag
    34801 gcggcataat cctgtaaacg tgatgattca ggatgagtta atgaaggatt ttcactaatg
    34861 cggatgaata tgtctgactt tgccactttc tttgccgtgg cccgtaatca aagctttcgt
    34921 gcagcgggcg atgaattagg cttatcctcg tccgccatta gccatagtat taaaacactg
    34981 gaacaacgtc ttaaaattcg cttattcaat cggacaaccc gaagcgtatc gttaacggaa
    35041 gcgggttcta atctctatga acgattgcgc ccagctttcg acgaaattca gatcatgtta
    35101 gatgagatga atgattttcg tctgacaccg acaggaacgc tcaaaatcaa tgccgcccgg
    35161 gtagctgcac gtatattttt aatgccttta ctggttggtt tcacacgtga atatcctgat
    35221 attaaagtcg agctcacaac tgacgattct cttgtcgata tcgtccaaca gggctttgac
    35281 gctggcgttc gacttagtgg catcgtcgaa aaagatatga tcagcgtggc gatcggccca
    35341 ccagtgaaat tatgtgtagc agcaacgcca gagtactttg caagatatgg caaaccccgc
    35401 catcctcatg atctgcttaa tcatcaatgt gttgtttttc gttatcccag cggcaaacct
    35461 ttccactggc aatttgcaaa ggagctggaa attgctgtcg caggaaatat cattctggat
    35521 gatgttgatg ctgaactgga ggccgttttg atgggtgctg gtatcgggta tctgctctat
    35581 gaacagatca aggagtacct tgatactggt cgtttagaat gtgtactgga agactggagc
    35641 acagagcgtt caggcttcca gatctattat ccaaaccgcc agtatatgtc ctgcggcttg
    35701 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
    35761 caataattat ttactattcg ctttacgcgc cagcagcggg aatatcagtc ctaatcctac
    35821 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaaggc
    35881 ttccattttc gggaagatcc ccgtcaagca ggcaaaggcg gtaaaggcaa aacaccaaat
    35941 cccgacggtc attgccagag gcttattacg aataaagaca tattctggtt tatatttctg
    36001 cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca gcggcatcac
    36061 taccgagtta agattcaaca gccatttata gagattgttc atatcaccga tgccgagagt
    36121 cggcagcata atcagaattg ccaccagcac cagggtcaga aaatagccat taacgggcgt
    36181 accagaagcg ttggtacgac ataaactggc tggaatatat ttgctgtcag catcacctaa
    36241 tagcacttta agcggggcat caatcgagaa taccagcgca gctacttgtc ccagggtatt
    36301 cgcaatggcg taaatcacca ttaaagtatt acccatgttg taatactcgc ccagcttctg
    36361 aaaggcgtaa tattgaccgt tagtcattaa gtcatccggg atattacgcg agtcaaacat
    36421 catccccatc gccagtgagc ccagaatggc acaaaccgca accatcaccg ccaggcatag
    36481 catccctttt ggaaattctt ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt
    36541 ctctgctccg ccaaccgcga aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat
    36601 atgaggaatg aacgtttccc aggtaatgtt tgtggtcgca atatgcactt cagtaatcgc
    36661 aggcgcggtt accgccatcg ccacatacag gagcgacata acaaacatcg caatccctgc
    36721 cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca taaagaagat
    36781 aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga ttagcgaacc
    36841 gtcgcctttc atcgcccaac cgagcgcaat cagaattgcc tggggttttt gtgccagata
    36901 gggaatatgc accacccagt aggtccacgc ggcgagataa gccagtccgg gtcccatcgt
    36961 gtgtttaatc caggtactga ccccgccctt cccatctttg aaggtcgagc ctaactgacc
    37021 aacaattagc gcataaggtg tgaaatagag tgcaaagata aacacccatg aaaaaacaac
    37081 caccagcccc tggttggcat agttattaac aacgttgcca aaaccccaga cagtgataaa
    37141 agacatcagc gcaatgttgt accatcgcaa ctgtttttcc tgaacattcc cagccataga
    37201 caaatccttc gccaaaatta aaggggcatc tgtaaaacga gggattatgc gcggcaaata
    37261 agaagtgcgg aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg
    37321 aataaaattt ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag
    37381 taagttcaca ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg
    37441 gcgttgcgca gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat
    37501 aaattatcgg agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc
    37561 acagtataac tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc
    37621 tcgcaagccc atgccatcgc caccggatat agcgtaaagc catcggcacc gaggatgaat
    37681 aacgctgggg ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag
    37741 acctgaacac gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcggccat
    37801 tgtccaagga tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc
    37861 acccctttgt gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct
    37921 gagataatgc agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt
    37981 gacgtcgaat catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag
    38041 atccctgcca acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact
    38101 ttgctgacca gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca
    38161 agcaaacgcc cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc
    38221 caaatcatgg cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca
    38281 atcatcaggc caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg
    38341 cggttaaagc caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg
    38401 gtaaaatagg atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc
    38461 caaagcggca cgagtgtatt taacaccgcg atcgccagag tcaacaaaag caggccagac
    38521 agcaaaagca tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa
    38581 atttcatgcg catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgcatat
    38641 cgcagcggcg gtattatcga tttatattac tcattcataa cgctaatgct gaagagacga
    38701 ttaatctcat ctgattgttt ttattgatct aacacaaatt tcatacgctt cgttcagtcg
    38761 aaaaatttca tggtcacagc aaactcatat ttcccgtcta tgctttcaga gcaatgcatc
    38821 gcgtctgacc gatttcagac gtaccactga ctctcgaaag gagaagagga tgaccaaacc
    38881 gtatgttcgt cttgataaaa atgatgctgc cgttttgctt gttgatcacc aggctggttt
    38941 actttccctt gtacgggata tcgaacccga taagtttaaa aataacgtgc tggcgctggg
    39001 tgacctggct aagtatttca acttaccaac tattcttacc accagttttg aaaccggtcc
    39061 taacggtcca ttagttccag aactgaaagc acaattcccc gatgcacctt acattgctcg
    39121 cccgggaaat attaacgcct gggataacga agattttgta aaagcagtca aagcgacagg
    39181 taaaaaacag ttaattattg ccggtgtggt aaccgaagtt tgcgtggcat tcccggcgct
    39241 ttcagccatt gaagaagggt ttgacgtctt tgtcgtgact gacgcgtcag ggacctttaa
    39301 tgaaattacc cggcattcgg catgggatcg catgtcgcaa gcaggtgcgc aattgatgac
    39361 atggttcggc gtggcgtgcg agctgcaccg tgactggcgt aatgatattg aaggactggc
    39421 gacgttgttc tctaaccata ttcctgatta tcgtaatttg atgaccagtt atgacacgtt
    39481 aacgaagcag aaataaaaga gaaccggggc cgaacccccg gttgttactg attagcttgc
    39541 gacggccatc cccacggtca tatgcaaacc atagaatacg ccgcgaccga ttaattcccc
    39601 tgccagaagc agaatgaatg agacggatag tagcggaacc gcaggctgat aaccttttag
    39661 ctgtggtgca atccacaagc acagggcaac ggccaaaagc acgatccgcc aggacatcag
    39721 cgcaccatag tccggcacca gcgctgcggc ctgctgcaca gaactatgaa tagttgccag
    39781 ctctgtgccc tgcatcactg acatcacacc actcaccacc agtgccagta cagaaatggc
    39841 tggcagcaga cgcatcgccc agccatcgac gccagccaga ctcagcaaca ggtaacccag
    39901 taacgggccg cccatgaaca tcgtcaggaa gaagcccatc ggcgtccaga tgctgtacca
    39961 ggtcggaacg gtatcaatgc tgttatacac acgcaccatc atccagacaa agacgacgcc
    40021 aagaaccatc gtcaccacca gccatagcgt acgcaatgcc ggtgacagct ttttcagcat
    40081 tgccagcaac cagccgatgc cgcctacagc aaaaaagatc gaaccgctgg cgatttcgtt
    40141 actgagtgct gaagccccta cccggttgag cgagttaaag gcgcgcatcg gtgaaccaag
    40201 atggagcata gaggcgataa agccaatgcc catcagcacc cataagccaa acatgcaggc
    40261 gataacacgc tgctgggctt ctgctcgcag gtcgcctttg agcagcgcca aagccagaac
    40321 gataaaacca cccgctacac attgcccgaa gaccgtgaag atcatcagcg gccattcatg
    40381 ccatccactt cccatctcac acctccttcg ggtttgccag gtagccagtg gtatccccgg
    40441 tcgggcggct gttggcattg ggtttgatca caatattcgg tttggtaaag tgagctcgcg
    40501 gcaacggcgc aacggccgcc agatcgccat gttttttacg cagctcgtcg ataggaccga
    40561 aatccagcgc ccgcagcggg caagattcaa cacagatcgg ctttttaccc tccgcaacac
    40621 ggtcataaca accatcgcat ttggtcatat ggcctttcgt ttcgttatat tgcggtgcgc
    40681 catacgggca agccatatga cagtagcggc agccaatgca tacatcttca tcgaccacaa
    40741 caaaaccatc ttcgcgttta tgcatcgcac cgctcgggca gactttagta caagccggat
    40801 cttcgcagtg gttacatgaa atcgacagat agtaggcaaa cacgttctgg tgccagacac
    40861 cgttatcttc ctgccagtcg ccgccagcat attcataaat gcggcggaag ctgacttctg
    40921 gcgtcaaatc tttgtagtct ttacaggcca gctcgcaggt tttgcaaccg gtgcaacggc
    40981 tggaatcaat aaaaaatcca tactgggttg tcatcggtta ctccttacac cttttcaacc
    41041 tgaacaaggt ttgtatgtga cggattcccc ttagcgagag gagacggacg ttgagtggtc
    41101 agtacgttaa tacaaccgcc cttatcgaca cgttttgcat ccgggtcata ccaggcacct
    41161 tcacccagtg cgaccacacc cggcatcatt cgtggcgtca ctttcgcctc aatatgaact
    41221 tcaccacgat cgttaaagat cctgacttta tcgccgttgt tgataccgcg tttttgcgca
    41281 tcaagcgggt tgatccacat ttcctgacgg caagccgctt tcagcacatc aacgttgccg
    41341 taagttgagt gaacgcgaga tttatagtgg aaacccgtaa gctgcagcgg atactgtttg
    41401 ttcagcggat cctgataact ttcaaagccc ggcgtgtaga tcggcagtgg atcgatcaca
    41461 tcgccttctg gcaattccca ggtagcggca atgtcagcca gcgcctgcga ataaatctca
    41521 attttgcccg atggcgtagt cagtgggttt gcctgcggat cttcacggaa ggctttataa
    41581 gcaacgtgat gcccttgtgg gtcgcgcttt ttaaagatcc cctgcttgcg gaactcttca
    41641 aacgttggca gttcaggaat cgcttcccgc gactgggcat acagatgccg catccactct
    41701 tcctgtgtac ggccttcagt aaactgttgc tcaacgccaa gacgttttgc cagttcgctg
    41761 gtcatttcat agatggtctt acattcaaag cgcggtttaa tcacctgatc gttgaaaatc
    41821 acgtaagaca tattcccgca ggatgcatcc agcgcaaagt ccatctgttc ggaagcggtg
    41881 cagtcaggca gcaggatgtc agcatatttc gccgatgagg tcatgtggca gtcgataacc
    41941 acaatcagct cgcacttctt atcatcctga aggatttcat gggtacggtt gatttcagaa
    42001 tgctggttaa tcaggcagtt accggcatag ttccagatca ttttgatcgg cacatccagc
    42061 ttatctttcc cgcgtacacc atcacgcagc gccgtcattt ccgggccacg ttcaatggca
    42121 tcggtccaca taaacatcga aatgctggtc tggatcgggt tttccaaggt cggcatacgg
    42181 acaaacggta agctgtatga accttcacgc gcgccgctgt tgcctccgtt aataccaacg
    42241 ttaccggtca gaatcgccag catcgagata gcacgggttg cgatttcacc gttagcgtga
    42301 cgctgcgggc cccatccctg gctgataaac gccggtttgg tactgccgat ttcacgagcc
    42361 agtttgatga ttttgtctgc cggaacaccg gtgatttgtg aggcccattc aggtgtttta
    42421 gccacgccat ctggcccttc acccagaata taagctttat agtggccatt tttcggcgca
    42481 ctggctggca gggttttctc atcgtagcca acgcaatatt tatcgaggaa tgcctgatcc
    42541 accaggtttt cagtgatcat gacgtacgcc agaccgttaa ccagtgcggc atctgttccc
    42601 ggacgaatag ggatccactc atcttcgcgc ccggcaccgg tgtcggtata gcgcggatcg
    42661 atgatgatca tgcgggcatt agatttctgg cgtgcctgtt caagatagta agtcacccca
    42721 ccgccactca ttcgcgtttc gccagggtta ttaccaaaca gcactaccag tttactgttt
    42781 tcgatatccg acgggctgtt accatctgcc cagccgccgt aggtatagtt caaaccttcc
    42841 gcaatttgcg cggaagagta gtcaccgtaa tggttcagat aaccgccgca gcagttcatc
    42901 aggcgcgcga ccagggtatt tcccgggggc caggagcggg tcatggtgcc gcccagcgta
    42961 ccggtgccat agttcagata gatggattcg ttgccgtact ctttgatcag gcgctgcata
    43021 ttggtcgcga tgatgtcgta ggcttcttcc cagctaatgc gctcaaattt gccttcaccg
    43081 cgcgccccga ctcgtttcat cggatatttc aggcggtccg gattatagac acggcgacgc
    43141 atggaacgtc cacgcagaca ggcgcgaacc tggtgcaggc cgtcgtaatt gtcatcgccg
    43201 gtattgtccg tttcgacata tttgatttca ccgtccacga cgtgcatacg tagcgggcag
    43261 cgactaccac agttaactgt acaggcgctc cagataacct tttcgtctga ttttgttgga
    43321 atggcgctat cgacagcgtg cgcaatccga ctaaaaggta atgttaatgc gctgctggcc
    43381 attgccaggc cgccgatcgc tgtcgttttt accaaaccac ggcgactcac ctcagcagcc
    43441 aataccgcat cagggatttt cgttttcata atggctcact caagcttgct cacgattgat
    43501 tccagtaaca aaccgtattg tgtaattatt tctatagcag ctcgctatat aggcttgtat
    43561 acatcgaata atttgaaaat caaagagata ggtagatttt cactcgaaag tagtattacg
    43621 tgtaagtgag gagtaattat tgttcggtat caaagggaga caaaaaaagc gcccgcaggc
    43681 gctttaacgt ttcttgctcc ccctgatgat ttacttattt ttcaacgaat aagccaaaac
    43741 agggggaaca aattaacaca aattataact aatcgatgga cagtttatgg acactttcag
    43801 ccagcggatt aaagttgatc gcgtcctgta aaaagtccgg cgaaaaatga gcgtacgtca
    43861 ttgtttgctg aatcgttgcg tgccccagga ttcgttgcaa cgtaacaata tttccgccat
    43921 tcatcataaa gtgtgtggca aacgtatgac gcatgacatg cgtagcctgc cccttcggca
    43981 aatccggttt cacctcctga agaatctccc ggaacttcag ataatcaacc tgatacaata
    44041 accccgttcg acgggtttta atcagaggca cgaccgaatc cgccacagga accgaacggg
    44101 attttccgtt tttggtgtta aagaacgtca cccggttgcc aacgatatgc tctccacgta
    44161 gttccgatgc ttcactccat cgggcaccag tagaaagaca cagaatggca acacgacgcg
    44221 catcaccctc cagtcgctca agaagccgtt caatctcatc atcagaaaga aaggccatct
    44281 ctgtgttctg aactttcagt tttcgtatac cgcgtacagg gttcgcgttg tgaaagacct
    44341 cagcttcaat cagcaccgtg aacatggtag acagtacgca taagtcacga ttaatactgg
    44401 acggcattaa tccggcctgt aatttctctg accgatattc aagcataaat tttcgcgtca
    44461 tctgactggc gcgaggatct cccatttcac gaacaacctt ccctagccta accctgtagc
    44521 tatccccgta agcctgatta cgcccatcaa gcatccacca cgcatcaagc agctctgata
    44581 atcgccgctg atcagctggc ttagcctgcc atggcttgtc atgaaagttc tgtaacacat
    44641 acttctcaaa ctcctgagcc tttgatttca gggcaaagat ttttcgaatc cgctttcctt
    44701 ccgccccctg cggtctgaca tcgacctgat aacgtccgtc tttgagttgc ttaatcgaca
    44761 tgattagccc ctccaacgga taaattaact aaacactcct tgcgatatgt aattcgctca
    44821 atgtgtagag tcagccaaca ttgcggcctg atcggggtga tttttgggta atgccgattg
    44881 aaacctgatc gccctccgac tgaatagagc catcaagaga gagagccggt gcaacctgcc
    44941 cggcagcggg aaatgttttg tcgtgaagta tccacatagt gtatttttcg aatcgctcga
    45001 cttggattac tttttctaca acctcaattc gcgcaggttt atatccagac tcataatttc
    45061 ctatagtccc tacagaaacc cccgttactt gcgaaaactg agattgtgta agtttttccg
    45121 catttctgat cgcctttagt tttttggcgt aatctcttga catgttcacc atacgatgag
    45181 tatcctcttt tcggtcttca ccacagggtg aagaaaacaa accaaaacaa accatcacaa
    45241 ggcgcaacaa gcgcaccaac gtaagaggat aacaaatgcg ggacttcatt gccgataaag
    45301 agttatctga ccaccaacca cttcaggaaa gtgacgcact cagcggatcc attgatgccg
    45361 ccaacgacga cgaacacgaa gaaaacaaga aaaagcgttc ctacaaaaaa ggagcgactt
    45421 tacggctgga tggtccgatc gccgggcttt gctctctgga aaaaggcgcg gcatatattg
    45481 ggctaacaaa atctgcactg cgcgtagcca ttcatcgcgg tcaaatgccg ggacacaaga
    45541 cgcgcacaaa cccggaagat gaaaactcag acggagtatg gtggtttaac gccaaagagt
    45601 gggacgcact ggctgacgag ctaccggaac ttgagccacc agaatggcac aactggaaaa
    45661 gttactggac gtatgaccgc cagaaaagaa agttctttcc agctaataaa gaagactgcc
    45721 agaccattaa cggtaagcga gtttatacag gcagaagttc aaagctggag cgactcaaaa
    45781 gcagcgaagg caactaaaca cacaaaaatg accagcaata tcatcagcgc cattctgaac
    45841 cttgtattcc tggaagtggg tgtaatagcg atttatctgc ttaacaagct aacaggacaa
    45901 aaagagcgct ttattgttct caacgttcat tacctcgccg cctataccag aggtcttttc
    45961 ccgaccacta ttggcgcggt gctcatagcg tttgttattt ggcttatcgg gtagaggcac
    46021 aaaaaatgaa taagcaacag cgaaacagcg aaacagcgaa acagcgaacc caataacatg
    46081 ggatttcgcc gcaattattt tgaaaatggg cgttatgccg gggaagtaaa caggtacgga
    46141 gtttttgaca tgaagcaaca gcgcaattct tcacaacagc gcttccgcaa cggcgcagaa
    46201 cgccacgcta accgcttcac taccagtgca tcacgtagca actctcgcta cagcctaagc
    46261 gagacgcacg caacgccaga tggccataca gtaaaacaaa ttggcgaaca cacttggctg
    46321 attgaaaaag ctggaatcgt agttcacaga tgcccacgca atccatttac cggaaaccgc
    46381 atttttgcat tgaactgcgg cgacaatcag ttcgggcagg atttcacatt atacgaagca
    46441 cttcgcacag ttgatcgtct gcttcgtggg caaagtttta ttaaacaagc tgatttataa
    46501 caggtgcttt atgactaaag accatgcaca aggtgtattt atccgcttta ttgattttcg
    46561 cggtgagttg ttgttacgcg catcagctat tgatgctgta gtcccggcag aaaaagatgt
    46621 ggccacttac ctttatctga acggcacgcg attgtttgtg gaacttccgt accagaccgt
    46681 acgcgaaatc attagcgaag ctgaaaaggc acgtcaggtt aatggcgatg aaccctatat
    46741 cgaaattatt tgtatggatt cagaagctga
//
LOCUS       DAHBHC010000033        46454 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHBHC010000033 DAHBHC010000000
VERSION     DAHBHC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.033"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            101..301
                     /gene="torI"
                     /locus_tag="M2A35_002271"
     CDS             101..301
                     /gene="torI"
                     /locus_tag="M2A35_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCB7198567.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(831..2078)
                     /locus_tag="M2A35_002272"
     CDS             complement(831..2078)
                     /locus_tag="M2A35_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCB7198568.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2150..3064)
                     /locus_tag="M2A35_002273"
     CDS             complement(2150..3064)
                     /locus_tag="M2A35_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCB7198569.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     LSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            3280..4713
                     /gene="cscA"
                     /locus_tag="M2A35_002274"
     CDS             3280..4713
                     /gene="cscA"
                     /locus_tag="M2A35_002274"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCB7198570.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4721..5779)
                     /locus_tag="M2A35_002275"
     CDS             complement(4721..5779)
                     /locus_tag="M2A35_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCB7198571.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            5959..6177
                     /locus_tag="M2A35_002276"
                     /pseudo
     CDS             5959..6177
                     /locus_tag="M2A35_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            6195..7523
                     /gene="dsdA"
                     /locus_tag="M2A35_002277"
     CDS             6195..7523
                     /gene="dsdA"
                     /locus_tag="M2A35_002277"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCB7198572.1"
                     /translation="MENDKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7631..9169)
                     /gene="emrY"
                     /locus_tag="M2A35_002278"
     CDS             complement(7631..9169)
                     /gene="emrY"
                     /locus_tag="M2A35_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCB7198573.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFTLALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(9169..10332)
                     /gene="emrK"
                     /locus_tag="M2A35_002279"
     CDS             complement(9169..10332)
                     /gene="emrK"
                     /locus_tag="M2A35_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCB7198574.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            10748..11362
                     /gene="evgA"
                     /locus_tag="M2A35_002280"
     CDS             10748..11362
                     /gene="evgA"
                     /locus_tag="M2A35_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCB7198575.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            11367..14960
                     /gene="evgS"
                     /locus_tag="M2A35_002281"
     CDS             11367..14960
                     /gene="evgS"
                     /locus_tag="M2A35_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCB7198576.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTNAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(15016..16161)
                     /gene="yfdE"
                     /locus_tag="M2A35_002282"
     CDS             complement(15016..16161)
                     /gene="yfdE"
                     /locus_tag="M2A35_002282"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCB7198577.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(16235..17179)
                     /gene="yfdV"
                     /locus_tag="M2A35_002283"
     CDS             complement(16235..17179)
                     /gene="yfdV"
                     /locus_tag="M2A35_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCB7198578.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(17249..18943)
                     /gene="oxc"
                     /locus_tag="M2A35_002284"
     CDS             complement(17249..18943)
                     /gene="oxc"
                     /locus_tag="M2A35_002284"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCB7198579.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(18997..20247)
                     /gene="frc"
                     /locus_tag="M2A35_002285"
     CDS             complement(18997..20247)
                     /gene="frc"
                     /locus_tag="M2A35_002285"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCB7198580.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(20760..21392)
                     /locus_tag="M2A35_002286"
     CDS             complement(20760..21392)
                     /locus_tag="M2A35_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCB7198581.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            21688..21963
                     /gene="ypdI"
                     /locus_tag="M2A35_002287"
     CDS             21688..21963
                     /gene="ypdI"
                     /locus_tag="M2A35_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCB7198582.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(22040..22282)
                     /locus_tag="M2A35_002288"
     CDS             complement(22040..22282)
                     /locus_tag="M2A35_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCB7198583.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            22635..23555
                     /gene="lpxP"
                     /locus_tag="M2A35_002289"
     CDS             22635..23555
                     /gene="lpxP"
                     /locus_tag="M2A35_002289"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCB7198584.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            23911..23982
                     /gene="ypdK"
                     /locus_tag="M2A35_002290"
     CDS             23911..23982
                     /gene="ypdK"
                     /locus_tag="M2A35_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCB7198585.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(24047..25285)
                     /gene="alaC"
                     /locus_tag="M2A35_002291"
     CDS             complement(24047..25285)
                     /gene="alaC"
                     /locus_tag="M2A35_002291"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCB7198586.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            25662..27359
                     /gene="ypdA"
                     /locus_tag="M2A35_002292"
     CDS             25662..27359
                     /gene="ypdA"
                     /locus_tag="M2A35_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCB7198587.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            27374..28108
                     /gene="ypdB"
                     /locus_tag="M2A35_002293"
     CDS             27374..28108
                     /gene="ypdB"
                     /locus_tag="M2A35_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCB7198588.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            28121..28978
                     /locus_tag="M2A35_002294"
     CDS             28121..28978
                     /locus_tag="M2A35_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7198589.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(28981..31476)
                     /gene="ptsP"
                     /locus_tag="M2A35_002295"
     CDS             complement(28981..31476)
                     /gene="ptsP"
                     /locus_tag="M2A35_002295"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCB7198590.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDRDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(31501..32537)
                     /gene="ypdE"
                     /locus_tag="M2A35_002296"
                     /pseudo
     CDS             complement(31501..32537)
                     /gene="ypdE"
                     /locus_tag="M2A35_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(32537..33622)
                     /gene="ypdF"
                     /locus_tag="M2A35_002297"
     CDS             complement(32537..33622)
                     /gene="ypdF"
                     /locus_tag="M2A35_002297"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCB7198591.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(33637..34884)
                     /locus_tag="M2A35_002298"
     CDS             complement(33637..34884)
                     /locus_tag="M2A35_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCB7198592.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGSPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(34906..35232)
                     /locus_tag="M2A35_002299"
     CDS             complement(34906..35232)
                     /locus_tag="M2A35_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCB7198593.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(35451..36416)
                     /gene="glk"
                     /locus_tag="M2A35_002300"
     CDS             complement(35451..36416)
                     /gene="glk"
                     /locus_tag="M2A35_002300"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCB7198594.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            36620..37876
                     /locus_tag="M2A35_002301"
     CDS             36620..37876
                     /locus_tag="M2A35_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCB7198595.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            37991..38317
                     /locus_tag="M2A35_002302"
     CDS             37991..38317
                     /locus_tag="M2A35_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCB7198596.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(38458..39696)
                     /locus_tag="M2A35_002303"
     CDS             complement(38458..39696)
                     /locus_tag="M2A35_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCB7198597.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            40032..41234
                     /gene="nupC"
                     /locus_tag="M2A35_002304"
     CDS             40032..41234
                     /gene="nupC"
                     /locus_tag="M2A35_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCB7198598.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(41284..43473)
                     /locus_tag="M2A35_002305"
     CDS             complement(41284..43473)
                     /locus_tag="M2A35_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCB7198599.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGGETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(43681..43756)
                     /locus_tag="M2A35_002306"
     tRNA            complement(43681..43756)
                     /locus_tag="M2A35_002306"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43721..43723),aa:Ala,seq:ggc)
     gene            43977..44336
                     /locus_tag="M2A35_002307"
     CDS             43977..44336
                     /locus_tag="M2A35_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCB7198600.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            44338..44730
                     /locus_tag="M2A35_002308"
     CDS             44338..44730
                     /locus_tag="M2A35_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCB7198601.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(44782..46197)
                     /gene="gltX"
                     /locus_tag="M2A35_002309"
     CDS             complement(44782..46197)
                     /gene="gltX"
                     /locus_tag="M2A35_002309"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCB7198602.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT        12450 a        11591 c        10707 g        11706 t
ORIGIN      
        1 ttttcattta tcaagatatc cagacctacc atcgccgcat caatgcggtt tttttattac
       61 ctgatttgca ggttcgattc catattcgga gatagcactc atgcaacacg aactacaacc
      121 tgattcactg gttgatttga aattcatcat ggctgatact ggctttggta aaaccttcat
      181 ctatgaccgg attaagtccg gcgacctgcc aaaagccaaa gttatccacg ggcgagcaag
      241 atggttatat cgtgaccatt gtgaattcaa aaataagctc ttaagccgcg ccaatgggta
      301 aaatagcggg taaaatattt ttcacatcta aaaaacacca ttccaatcaa tcccctgccg
      361 cttcaagcag atgtctgcag gggacaccag atacccttca aacgaaatct accttcaccc
      421 cgtaaaagat gggtttggca gcacacttgc cttatatcta ctcattttta ctgcaacagg
      481 ttgaaatctc agcactgtca gaaagcgctg atgactaaac agccctgggc cgggcgatgt
      541 aaccatcaca cagaatcctg atagcgaaat atggcgtgac tcgatacttc actccgcaat
      601 gcattccttg atgaattcgc aggaccgtga tacacgggac aggtcactga atgacgacaa
      661 tgtcctggaa atcagcgaac cgcgcatctg aagtacattt gagcgactgt accagaacat
      721 gaatgaggcg tttggattag gcgattatta gcagggctaa gcattttact attattattt
      781 tccggttgag ggatatagag ctatcgacaa caaccggaaa aagtttacgt ctatattgct
      841 gaaggtacag gcgtttccat aactatttgc tcgcgttttt tactcaagaa gaaaatgcca
      901 aatagcaaca tcaggcagac aatacccgaa attgcgaaga aaactgtctg gtagcctgcg
      961 tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tcccaagcga actggcaatt
     1021 tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaagtttgc cacgctgtat
     1081 ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacaactt cactaatgaa
     1141 ataatccagg ggttaacgaa cagcgcgcag gaaaggatac gcaacgccat aatcacaact
     1201 ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat aatcgccatg
     1261 cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg cgttcctaca
     1321 tcgtgtgatt cgaataaacc tgcataaaag acaggaaaaa gttgttgatc aaaaatgtta
     1381 tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg atccttgaaa
     1441 actgcgataa aatcctcttt ttttacccct cccgcatctg ccgctacgca ctggtgatcc
     1501 ttatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagcga gaccaaccag
     1561 aagttgatat ggggactgat actaaaaaat atgccggcaa agaacgcgcc aatagcatag
     1621 ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg cgccattttt
     1681 tcggtgaagc tatcaagcaa accgcatccc gccagatacc ccaagccaaa aaatagcgcc
     1741 cccagaatta gacctacaga aaaattgctt tgcagtaacg gttcataaac gtaaatcata
     1801 aacggtccgg tcaagaccag gatgaaactc atacaccaga tgagcggttt cttcagaccg
     1861 agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa ctggttgacc
     1921 gaataaagtg tacctaattc cgtccctgtc aaccctagat gtcctttcag ccaaatagcg
     1981 tataacgacc accacagcga ccaggaaata aaaaagagaa atgagtaact ggatgcaaaa
     2041 cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct gtcgttaaaa
     2101 aattcacgtc ctatttagag ataagagcga cttcgccgtt tacttctcac tattccagtt
     2161 cttgtcgaca tggcagcgct gtcattgccc ctttcgccgt tactgcaagc gctccgcaac
     2221 gttgagcgag atcgataatt cgtcgcattt ctctctcatc tgtagataat cccgtagagg
     2281 acagacctgt gagtaacccg gcaacgaacg catctcccgc ccccgtgcta tcgacacaat
     2341 tcacagacat tccagcaaaa tggtgaactt gtcctcgata acagaccacc acaccttctg
     2401 cacctttagt caccaacagc atggcgatct catactcttt tgccaaggcg catatatccc
     2461 gatcgttctg tgtttttcca ctgataagtc gccattcttc ttccgagagc ttgacgacat
     2521 ccgccagttg tagcgcctgc cgcaaacaca agcggagcaa atgctcgtct tgccatagat
     2581 cttcacgaat attaggatcg aagctgacaa aacctccggc atgccggatc gccgtcatcg
     2641 cagtaaatgc gctggtacgc gaaggctcgg cagacaacgc aattgaacag agatgtaacc
     2701 attcgccatg tcgccagcag ggcaagtctg tcgtctctaa aaaaagatcg gcactgaggc
     2761 ggaccataaa cgtaaatgaa cgttcccctt gatcgttcag atcgacaagc accgtggatg
     2821 tccggtgcca ttcatcttgc ttcagatacg tgatatcgac tccctcagtt agcagcgttc
     2881 tttgcattaa cgcaccaaaa ggatcatccc ccacccgacc tataaaccca cttgttccgc
     2941 ctaatctggc gattcccacc gcaacgttag ctggcgcgcc gccaggacaa ggcagtaggc
     3001 gcccgtctga ttctggcaag agatctacga ccgcatcccc taaaacccat actttggctg
     3061 acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct ttttcgcaaa
     3121 atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag ttaacgttaa
     3181 catttgtgat attcatcgca tttatgaaag taagggactt tatttttata aaagttaacg
     3241 ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc tcgattgcat
     3301 gcggcgcaaa acgccctagc aaaacttcat gagcaccggg gtaacacttt ctatccccat
     3361 tttcacctcg cgcctcctgc cgggtggatg aacgatccaa acggcctgat ctggtttaac
     3421 gatcgttatc acgcgtttta tcaacatcat ccgatgagcg aacactgggg gccaatgcac
     3481 tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat tgcgctagcg
     3541 ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga tgacaatggt
     3601 gtcctctcac ttatctacac cggacacgtc tggctcgatg gtgcaggtaa tgacgatgca
     3661 attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga gaaacagggt
     3721 gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt gtggcgtgaa
     3781 gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg gcagatcctg
     3841 ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc ccacgctgat
     3901 gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga tcagcattat
     3961 ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa tcgctttcaa
     4021 agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc cgggcatttt
     4081 actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc gaaggatggt
     4141 cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc aaaacgtgaa
     4201 ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg caaacttcta
     4261 caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt ctctccccgc
     4321 acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat tcagttgcag
     4381 tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac tggaatgcgg
     4441 ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc acacgagaat
     4501 ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct aaggatattt
     4561 atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat gagtagtcga
     4621 atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg agtggctgtg
     4681 ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa caacggatca
     4741 acagcgggca agggatccgc gtcactcttc ccccttcacg accttcaata atatgcaatg
     4801 cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc ggtaaaaaca
     4861 gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc gggatacgta
     4921 cccccttcgc caggagaacc tgataagcga caaaggctgc gcgatcgtta ccacatatca
     4981 gaacatcaaa atctggtttg cccggtttga agtgggcatt gagtaaactt gcgagatcgg
     5041 tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga tctcgtccag
     5101 catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac gcactttccg
     5161 gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt gttgattcat
     5221 attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc gccacacagt
     5281 tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt cgcagcccca
     5341 ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc gcgcgggcag
     5401 cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg ctggcggttt
     5461 gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc tgcgccagca
     5521 cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa agatcaggaa
     5581 cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga cgcacagatt
     5641 ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg cgtgcgacat
     5701 cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag cagaaacaga
     5761 cgtcactggc gagatccaac gatattctca acgccctatc acccaacacg gcaatacaat
     5821 aaaaaataac aataattgcc ggataattgt actcaattcc gcctctgttc tcgccaacga
     5881 gtaaccatat aggcaagtaa gtaaaacatg ccaactggca tcgccaacct ggagaagaaa
     5941 atataaatat ggatcattgt gctgccgctg tatcccgaca tcagcccgga aattattgcg
     6001 attgctatcg gttcaggtgc aattggctgc acgatcgtta cggactcgct tttctggcta
     6061 gtgaagcaat attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca
     6121 tttatcgctt cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg
     6181 caaagagacg tactatggaa aacgataaaa tgaactcgct catcgcccag tatccgttgg
     6241 taaaggatct ggttgctctt aaagaaacca cctggtttaa tcctggcacg acctcattgg
     6301 ctgaaggttt accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct
     6361 tatcccgttt tgcaccctat ctggcaaaag catttcctga aactgctgcc actgggggga
     6421 ttattgaatc agaactggtt gccattccag ctatgcaaaa acggctggaa aaagaatatc
     6481 agcaaccgat cagcgggcaa ctgttactga aaaaagatag ccatttgccc atttccggct
     6541 ccataaaagc acgcggcggg atttatgaag tcctggcaca cgcagaaaaa ctggctctgg
     6601 aagcggggtt gctgacgctt gatgatgact acagcaaact gctttctccg gagtttaaac
     6661 agttctttag ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg
     6721 gcattatgag cgcccgcatt ggctttaagg tgacagttca tatgtctgct gatgcccggg
     6781 catggaaaaa agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt
     6841 atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta
     6901 ttgatgacga aaattcccgc acgttgttcc ttgggtattc cgtcgctggc cagcgtctta
     6961 aagcgcaatt tgcccagcaa ggccgtatcg tcgatgctga taaccctctg tttgtctatc
     7021 tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg
     7081 gcgatcatgt tcactgcttt tttgccgaac caacgcactc cccttgtatg ttgttaggcg
     7141 tccatacagg attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg
     7201 cagcggatgg ccttgcagtt ggtcgcgcat caggctttgt cgggcgggca atggagcgtc
     7261 tgctggatgg cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg
     7321 cgcaggaaga aggtattcgt cttgaacctt cagcactggc gggtatggcc ggacctcagc
     7381 gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt cagcgcagaa caattgcgta
     7441 atgccactca tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatgaatc
     7501 aatatctggc aaaaggccgt taataacgtt tcaacgcagc atcgcaatcc tttccctggg
     7561 tgagcgatgc tgccgatggc gcagacttaa gatccccggt cttacccgct ataaccccct
     7621 ccttttatca tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa
     7681 ccgtaagtaa gataaaagca atagccgcca tacggaaaat ctcatttgca gaaatagaaa
     7741 gtgactgctg ggttatttca ttattaattt cattaagaac tcctgaaagc gaaccatagt
     7801 atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg
     7861 tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac
     7921 caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat
     7981 tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga
     8041 atccctgaaa aaactgcggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca
     8101 cagaacgcca gtaatagcaa accgcataca tcaaaaaact aaatgtcact aacaaccgca
     8161 tgtctatttt gttgccataa cgtcctatca aaggtgaaat taatagtggc atgatgccga
     8221 tgggcgcata agcaagtccg gcccatatcg cattataccc catcgtttcc tggagtaact
     8281 gcggcataag gacgatcgct ccagagtaaa ataaatacgc gcatgtgata ctcacaatac
     8341 caatggtgaa gttacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg
     8401 actcccaaat gactaaagag atcagagaaa taactgatac tactgttaat attattattg
     8461 tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cataatttgc aagccaccaa
     8521 caccgagcac taacagggtc agtcctggta gattcatttt gaccggtgaa gtttcagttt
     8581 ctcttccttt aagtaaggtt aagcataatg tcaggacgat aatccccata gggacattga
     8641 ttaaaaatat ccaaccccag ctaaagttat cgcaaatata accgcccaat atcggcccac
     8701 atatcggagc gataatcacg gtcattgacc ataatgccag agtaaatgtt cttttttctg
     8761 gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc
     8821 cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg
     8881 aagacagcga aaaaaaagtg actgaaagta aaaataacct taattcgcct attctttgtg
     8941 ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgag gtgataaccc
     9001 aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag
     9061 aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata gtaacgcacc
     9121 ataacgtccc accggttaat ggtgccggag tcgatttagt gattgccatt aaagttgtcc
     9181 attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc
     9241 cttactggta taagccggca tggacgtcac ggttgaagcc agatcaggca tctcagcaat
     9301 gtcttcgttc ttcgtatcaa tagttgcagt catcgataaa ccaatgcgta aggggtgttc
     9361 catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca
     9421 gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat
     9481 ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac
     9541 tgattgacca atccgtacat ccgtgagttg tgtttcttta aagttggcat taacccacat
     9601 ttgacgtgcc ggtacgacag ccattaacga ttgtccggga ctcactgttt cgccgacctg
     9661 aacactccgc tgggcaatat agccagtaac cggactcctt atatccgtac gtttaagcgc
     9721 caaccaggct tctttagttg catccgccgc ttccacgact tgtggctgac ggtttaatgg
     9781 tgtgttcatt actaaagctt tattcgcttt ataagcctgg atagcggcat tcagtgccgc
     9841 tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg
     9901 cttcgctaac ggtactcgac ggttataatc ttctaaagat tgttgatact gaatacgtgc
     9961 tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac
    10021 aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag ttttatccag
    10081 tgaaactaaa atgtcacctt gtcgaacgta gttcgtatct ttatgattaa cgacagtgac
    10141 actacctgag acttgtgcag aaattggatc tgcattcccc gtgacatagg cgtcatctgt
    10201 actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa
    10261 taaaactacc gccaataaag aaaagtattt tcttctgtta gaatgttttt tatttgaatt
    10321 aatctgttcc actattatct ctcatttctc atagatgaaa tttatgaatt gaatgaaaag
    10381 agcattcatt cttcatatca aacacagtgc atgtgttgca cattatccca ggaacgttgg
    10441 aggcctaatt taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa
    10501 tacaattctt acgcctgtag gattagtgag aagacttata gtgctaactt gaaactataa
    10561 atcatcggta taatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg
    10621 gtatacttgt cgaattatct taaaggaagc tcagattttc ttatttttat tgagaaaatg
    10681 agatgacgcc ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga
    10741 ataatctatg aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa
    10801 tttattgatc aaaaacgata ttgaaatctt agcggagttg actgaagggg gaagcgccgt
    10861 tcagcgggtg gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt
    10921 taacggtatc caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat
    10981 tgtctccgct aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg
    11041 attcgtgagt aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa
    11101 tggctactgc tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca
    11161 gcaaaaactc gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg
    11221 caaggataat aatgacattg ctgaaaaaat gttcatcagc aacaaaactg tcagcactta
    11281 taaaagtcgc ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc
    11341 gcaacgtaac aaaatcggct aatcacatga agtttttacc ctattttttt cttctctgtt
    11401 gtggtctttg gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca
    11461 gtagtaacaa ccgtgtcaca cttgatccag tacgtctgag caacaaggaa ttacgttggt
    11521 tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc ccaaacggct accttgttgc
    11581 ataccgattc gcagcaacag gttcgtggta ttaatgctga ttatttaaat cttttaaaaa
    11641 gagcattaaa tatcaaatta acactccggg aatacgccga tcatcaaaaa gcaatggacg
    11701 cgcttgcaga aggtgaagtc gatatagtgt tatcacattt agttacttcg ccgcctctta
    11761 atagtgacat tgctgcaacc aaaccattga taattacctt tccggcgctg gtaaccaccc
    11821 ttcacgactc aatgcgaccg cttacctcac caaaaccagt aaatattgct cgggtagcaa
    11881 attacccccc agacgaggta attcatcaat cgtttccaaa agcaacaatt atctctttta
    11941 caaatttata tcaggcatta gcatccgtat cagccggaca gaatgattac tttattggta
    12001 gtaacatcat taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga
    12061 aatattataa ctcgccgcgt caatataatt ttttcttgac cagaaaagaa tccgtcattc
    12121 ttaatgaagt actcaataga tttgttgatg ctttaacaaa tgaagttcgc tatgaagttt
    12181 cacaaaattg gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg
    12241 aacatgaaaa acagtggatt aagcaacatc ccgatttaaa ggtgctggaa aatccttact
    12301 ctcctcccta ttctatgacg gatgaaaatg gctcggttcg gggcgttatg ggggatattc
    12361 ttaatattat taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata
    12421 tccatgccgg aacacagctt aaccccggtg gatgggatat aatacctggc gctatttata
    12481 gtgaagatcg agaaaataat gtttcatttg cagaagcttt cataacaacg ccatacgttt
    12541 ttgtcatgca aaaagcacct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca
    12601 ttccatatta ttatgagctg cattcgcaat taaaagagat gtatccggag gttgaatgga
    12661 tacaggtcga taatgccagc gctgcatttc acaaggttaa ggaaggtgaa cttgatgctc
    12721 tggtcgcgac acagttaaat tcgcgttaca tgatcgatca ttactatcct aatgaacttt
    12781 atcattttct tattcctggc gttccgaatg catcgctttc gttcgctttt cctcgcggag
    12841 aaccggaact taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc
    12901 tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctat
    12961 atagcgagca attttatatt gttacgacat tatccgtttt attagttggc agtagccttt
    13021 tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag
    13081 aaaaccaaat atcattccga aaagcactct cggattcctt accgaatcca acttatgttg
    13141 taaactggca aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg
    13201 attactacaa aaatgcaatg ttaccattag aaaacagtga ctcacccttt aaagatgttt
    13261 tttctaatac gcatgaagtc acagcagaga cgaaagaaaa tcgaacaata tacacacagg
    13321 tatttgaaat tgataatggc atcgagaaaa gatgcattaa tcactggcat acattatgca
    13381 atcttcctgc aagtgacaat gcagtatata tttgtggttg gcaagatatt actgaaacgc
    13441 gtgatctaat taatgcactc gaggtagaaa aaaataaagc gataaaggct accgtagcaa
    13501 aaagtcagtt tctggcaacg atgagtcacg aaataagaac accaataagc tctattatgg
    13561 gcttcctgga acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt
    13621 cacttgccta cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg
    13681 acaaaattga atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag
    13741 tccagaacac ttgtcactct ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt
    13801 gcagcagtac atttcctgat cattacctgg ttaagatcga ccctcaggcg tttaagcagg
    13861 tcttatcaaa tttactgagc aatgctctca aatttaccac cgagggggca gtaaaaatta
    13921 cgacctccct ggttcacatt gatgacaacc acgctgttat caaaatgacg attatggatt
    13981 ctggaagtgg attatcgcag gaagaacaac aacaactctt taaacgctac agccaaacaa
    14041 atgcaggtcg ccagcaaaca ggatctggct taggcttaat gatctgcaaa gaattaatta
    14101 aaaatatgca gggcgatttg tcattagaaa gtcatccagg cataggaaca acatttacga
    14161 tcacaatccc ggtagaaatt agccagcaag tggcgactgt cgaggcaaaa gcagaacacc
    14221 ccatcacact acctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc
    14281 tattactcaa acgccagcta aatctattag gatatgatgt tgatgaagcc actgatggtg
    14341 tgcaagcgct acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata
    14401 tgccgaatat ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac
    14461 ccatctgggg gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg
    14521 gcatgaactt atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc
    14581 agttacacca ggttgcgcat attgcacctc agtatcgcca ccttgatatc gaagccctga
    14641 aaaataatac ggcgaacgat ctacaactga tgcaggagat tctcatgact ttccagcatg
    14701 aaacgcataa agatctaccc gctgcgtttc aagcactaga agctggcgat aacagaactt
    14761 tccatcagtg tattcatcgc atccacggtg cggctaacat cctgaatttg caaaagttga
    14821 ttaatattag ccatcagtta gaaataacac ctgtttcaga tgacagtaag cctgaaattc
    14881 ttcagttgct gaactctgta aaagaacaca ttgcagagct ggaccaggag attgctgttt
    14941 tctgtcagaa aaatgactaa atagcggctc ccacaatgtt caaatgtggg agctatttac
    15001 cagcacatct tacatttatg atgagaactc ctggcgaatt tgttccccat gctggtcgag
    15061 cgttgccgct cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc
    15121 cggcatcatt attcccccgg cttcaatcaa catatttctc gcctgagttt gtggcaattt
    15181 aatggcctca gccacactta ataacggcgc gacgggtaca ccaacttcat gtattctggc
    15241 taaccaaact tcagctgcct gcgtttttaa cgtccgctca atatattgtt taagaatagc
    15301 ctggttttgt acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag
    15361 ctccagtgcc tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg
    15421 cttatcctga gtattgaaaa catcaaaagg tgccatgtag ggatggcgat ttcccagacg
    15481 ttgtggtgac ttcccagttg cgatatatgc catcagacca tgctccagaa aactcagcgt
    15541 ggcatcaaac atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag
    15601 tgcactcact attccgctga ataaatagac accgccgcat agatccgcaa gagaggtacc
    15661 aacgcgcact ggcggagcat caggatatcc tgtttccatc attatcccgc tcattgcctg
    15721 aatgatggta tcgtaggcag gagcatcttt tagcggaccg gtatgtccga aacctgacga
    15781 tgaagcatat atgaggcgcg ggttgatttc ttgaagcgtt tcccatgaaa accccagttt
    15841 ttccattgta cctgggcgaa aattctcagc taatacatca gcttgtttga gcatatttat
    15901 aaatatactt ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt
    15961 aataaaactg taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc
    16021 accatgaccc ggcggctcaa ctttaattac ccttgcgccc atattacaaa gaagttgagt
    16081 tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc
    16141 tttgctttca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac
    16201 ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa
    16261 tccacaaggg agccgtgacg acaaagccca gtacgctcac agccaatgac gctgtaccag
    16321 tacgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaatg
    16381 cgcctgccag taccatcatc tgcagatgtt cgctgttcaa atgacatgcc ataccaacga
    16441 gaagcagtgc cagtggcatc agaatcagct tcaggaaggt gttataagca atttcggcac
    16501 tgaactcgaa tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt
    16561 tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca
    16621 acaccaggat cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta
    16681 atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca
    16741 gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctacc actaaacctg
    16801 ttgatacgga atcaccataa ataggatcga gaactgcaaa ccccaggaat ccaatggtag
    16861 gtgaacccgc aattaatgca catacagctg cttctgcatg ggtacgttta aaaaatttgt
    16921 agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca
    16981 gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagagccg
    17041 caggaagagc gtagtttaat accagtttat taaatgcgcg ggcttgatct tctgaaaaag
    17101 tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc
    17161 caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca tcgcataatg
    17221 agttggcaag aggagacgat gtcagtattt aattaccagc gacttgtttt gggttaagtt
    17281 tggtaatatg gccactttca gttcctgctg cagggtcgat gaccacatta ataatggtcg
    17341 gtttgcgcga ctgaataccg gtggttaaag catgacgaag ttcatctgtc gtggtgacgt
    17401 tatagccaac gccacgaaac gcatccatta atttgtcata ccttgcatgg tgcaacagat
    17461 ccgttggtga tggtgcacca gcgccactga gatcaacacc gtctcctctg tagatgccgc
    17521 cattattaaa aataacgatc gtcaccggca ggttatatcg acaaatcgtt tcaatttcca
    17581 tcccactgaa accaaaagca ctatcacctt caatggcgac aaccggagaa ccagaagtca
    17641 cgctagcacc gatggcatag cccataccga tgcccatgac accccaggta ccacaatcca
    17701 gacgacgacg tggtttatac atatcaataa tatttcgtgc attatccagg gtatttgcac
    17761 cttcattaac taaataaata tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg
    17821 cattaaagta atttaatggt tgagtatctg tacttaattt ttcatgcatt ttttgtgcat
    17881 tttgctgctt gtggatattt aaaatatcgc gccataccag tggagtcgta aatgtgtttt
    17941 gtttcagttc tgccagcata ccttgcatac tggatgcaat atcaccaacg actggcacag
    18001 caatggggcg gttgctgtca atttcctgcg gttcaatatc cagttgaata aactgtgtat
    18061 ctgccgccca tcctttttta ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca
    18121 tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg
    18181 tatcttcaag gatccctttc gccatagaca ttggcaggaa tggaatctga gtactttcaa
    18241 taaattcacg aagctgttca tcagcttgtg aatacgccgc gcctttgcca aggataatta
    18301 atggccgttc agcttttgct aaaagcgaaa ttgcgctagt gactgacttc gggcatggca
    18361 ataatgctgg cgacggattt tcaactttaa caatcgtggt taacgcttcg tctttttcca
    18421 tcgtcgcggc caggacattt gctggcaaat caagataaac tccgccaggg cgacccgata
    18481 cagagacccg gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa
    18541 atgctgcttt ggcatacggt tttgccgcat tcatttggtc cagctcttca taatcacctt
    18601 gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat catgatcatc ggaaaaccat
    18661 ttaccgttgc gttggccaat gcggtcaaac cattgaggaa tcctggcgca gaaactgtca
    18721 ggcagatccc cggtttttgg gtaagaaaac cgcttgccgc agcggcatag cctgccgact
    18781 gctcatgacg aaaaccaata taacgaatgc cttccgcctg ggcatggcgt gccatatccg
    18841 tcacaggaat acctacaaca ccataaatag tgtcaatatt attctgtttt aatgcttcaa
    18901 cgatgatatg cataccatct gtcatttgaa gttgatctga catgattaat aacctcgaaa
    18961 tttttataaa ttgggagcca gaagcccccg ttaatatcag atggcgtggt tttgcttcat
    19021 tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat acagcagcgg tatgttcacc
    19081 taatagcggc gcagctttaa tatccggcgt aaaggcagag aatttcattg gacaaccaac
    19141 ggtcagatat tttccacgca acggttgttc cacttcaaca acactgccac tttggcgcaa
    19201 agagggatca agtgaaattt ctttcatact taaaaccggt gcacaaggaa tatcaaactg
    19261 agtcaaatag gccaccgctt catgtttatc aatagtgaca gtgtattttt cgatttcagc
    19321 aaaaatatcg aaaatatgtg gctgtcgtgc atgggctgta ctgtatgccg gatcggtaat
    19381 ccattctggt ttgccgatgg ctttacaggt gttttcccag ttttgctcct gaatagtgaa
    19441 ataaatatag gcgttaggat cggtttccca gcctttacat ttcaggatcc agccaggctg
    19501 accgccacca cctgcattac caccgcgggg aactgcatca ccaaatgtac cattcggata
    19561 ctgcgggtat tcttccagat aacccaattt atcgagacgc tgctggtcac gtaatttcac
    19621 gcggcaaagg ttcaatacgg catcctgcat tgacatggtg actcgttgcc cacgccccgt
    19681 tttttcgcga tgcagcaaag cagcaagtaa accgatcagc aaatgcattc cggtgttgct
    19741 gtcacccaac gctgcagcgc ttaccagcgg cggaccatcc caaaaacccg tagtggatgc
    19801 cgcgccaccc gctgcctgag caacgttttc ataggctttt acattcacat aaggcgaaca
    19861 ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga ttgatttctt gaatatgctc
    19921 ccaggtgaag cccatgtgat caatggcccc tggatgaaag ttctcgacta agatatcagc
    19981 ttcgcggatc agcttttcca ttacctcttt gccttccgct gttttggtat ttaactcaat
    20041 agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg
    20101 cagctggtga cgcgttacgt caccaacgcc gggacgttca attttaataa cgtcagcgcc
    20161 aaaccaggcc agcatttgag tacaagatgg gccagattgc acaccggtga aatcgagaac
    20221 tttaattcct tgaagtggag ttgacatagc aatacctatt agttaattaa atatgtgtta
    20281 atttagcgtt tatttaaggc gtaatatctt ctataaataa cccgcaaaat atcgtcgtag
    20341 ctgcactcga tatttttaaa atcctgtatc aagtgctgaa aatcatttca tcgccacttc
    20401 caattccttt ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattgatt
    20461 cgtggtaatc agagagagtc gcgacatttg tgatgttatt cttacaactg taagaagcgc
    20521 cggtgattta caagtaaggg tatgattacg ctttttaaag cgtaccataa aacataacat
    20581 aataaatgat ttgtgatgac acgggattat tgggatttac tttgaggaag ataaaatgcg
    20641 tgtttataga atcgcgagcg ccaaataatt ttatgaccac aacctgtcgt attgtggtca
    20701 taaaaaattg tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcat
    20761 tagttcacaa acagtgcttc gctgtcgacg atgattccat cttccgcacc tttaagcgcc
    20821 aggtttgcct cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta
    20881 cgagtctgtt taagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga
    20941 agttcttcca ttgcgccctt tttatcccct ttcgccattt tttcattggc aatttttatc
    21001 gcggcttctt tttcgggagt tgctacataa ctctcagaaa ttccgacaga tgcattaatg
    21061 acaatatatt ggtcatcatt gagattggtc tttttacctg gcttagcgaa ctttgcccat
    21121 tcggtactgt catctgacag caaagcggaa gcttcattgg tcagttcttt tgccttttct
    21181 ggatcgccat gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct
    21241 tgttcagaga tacgctctgc tgctgccgtt ttctgggttt gctgaacttg ctgagtttgc
    21301 tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct
    21361 gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa cagttttaga
    21421 aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt
    21481 gaaagggtta tcttacagtt gtagttatta tcgcaagaag tattttagtc ttacaattgt
    21541 aggaatctgc ttcctgtcgc agagaactat tcttatgtac cacaaagaga tgtcatttat
    21601 tatatcaatg taaatattga ttaattatat gtaaatacag ccataacatc gtaggttctc
    21661 agtgaaccat tttcaggata actttctatg aaagtaaact taatactttt cagcttattt
    21721 ttattggtct ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt
    21781 gagaggagct ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa
    21841 cttcagccat cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc
    21901 acagaagaaa tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga
    21961 tagcgtctgt cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca
    22021 aatgagccat cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc
    22081 cacaagccgg ggcaagaaac ataaatatcc ccgtcactag ttcattaccg ccagttaacc
    22141 acatggtgaa gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg
    22201 ccgataccac gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga
    22261 gaaacatcca taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc
    22321 agtcacttta atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa
    22381 aacagcatgt taaatgcaag actgttgtgt acggaaaaat atttactttg cacgattaat
    22441 aatcagtagc tgaaagcagt cagcgagtga taaagtacag ataccgcatt catgctcctc
    22501 gtggttatgt cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc
    22561 ccaaccaatt gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc
    22621 tacagaaggt aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct
    22681 actggctcac atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg
    22741 ttctctgctt tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg
    22801 aatctatcgc ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg
    22861 agaagatgat tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg
    22921 catggttctg gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc
    22981 ttaaacgcgc acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc
    23041 tggaactggg cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac
    23101 ataataatca gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga
    23161 tgatcggcag aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat
    23221 ggtttgctcc cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg
    23281 tggaaaatgt cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca
    23341 tgttgaccgt aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc
    23401 cagagatgga aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta
    23461 tcgagaaaga gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa
    23521 cgcgcccggt gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc
    23581 tcaaatcggc cataataacc ttatggtttt tatggctttt ttcttaaggt tttttccttc
    23641 gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa
    23701 gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag
    23761 cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc
    23821 ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg
    23881 cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt
    23941 atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga
    24001 aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt
    24061 cgtgaatatg tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc
    24121 cacgaatcgc ctgacgaata cggtcgcggt tttcgatcag tgcaaagcga acatgcgtat
    24181 caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct
    24241 tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat
    24301 acatcgaagc cttcggcatt tcaaccatcc agcccgcttc atgcagccct ttaaccaata
    24361 catcacggcg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcca
    24421 gcgccgcaat cgccgccacc tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa
    24481 tacgagccag ggcgctgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg
    24541 ccatgttgta gcttttcgac agcgtaaaga actcgactgc cacatcgcgt gcacccggta
    24601 cctgcatgat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcgt
    24661 ggaccaccag cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagct
    24721 ccacgcattg cgcggttgga ttcgacggga agccgaggat catcatcttc ggtttcggat
    24781 aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca
    24841 ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat
    24901 tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct
    24961 ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat
    25021 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac
    25081 catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg
    25141 caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca
    25201 ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc
    25261 gacgttcagg gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc
    25321 cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
    25381 cagcgtttta gaacattttt atcaatggct ttaaatgagg aactccactt caaaaacagg
    25441 aaatttttct acgctaacaa gaaaattacc accaatttta ttaacattat tatttcaata
    25501 aatttaccag ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc
    25561 aatccccttt taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag
    25621 gttacctatc atagaggttt aatccttgtt cagagtcacc cgtgcacgaa atattcaaca
    25681 tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc
    25741 gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg
    25801 ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag
    25861 aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc
    25921 cgtgggtagg catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg
    25981 gcggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct
    26041 ggataaacct gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt
    26101 tgtgtgagac gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga
    26161 tcgatatcgt ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta
    26221 ttgtgcttct ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca
    26281 agctggcgct ggatatcgcc aacaaaacgc tacctctgtt tcgccatgtc aatagcgagt
    26341 cattacgcaa agtttgtgaa attattcgcg atgacattca cgccgatgcg gtggcgatta
    26401 ccaataccga tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg
    26461 atgacttcat tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa
    26521 acaatgatga agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg
    26581 agaaaggcgt tgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct
    26641 cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat ctccacccaa ctggaggttt
    26701 cacgcgccga gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca
    26761 aaattaatcc ccatttcctg tttaacgctt tgaacgctat ttcatcatca atccgtctga
    26821 atccggatac tgctcgccag ctgatcttta atctgtcgcg ttatctgcgc tataacattg
    26881 aattaaaaga cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata
    26941 ttgccattga gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag
    27001 aggtgaattg ctgcattccc agcctgctga tccagccgtt ggtggagaat gccattgtcc
    27061 acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggca
    27121 atcgggtacg cattgcggtg cgggataccg ggcacggcat cgatccaaag gtgattgagc
    27181 gggtcgaagc gaatgaaatg ccgggcaata aaattggcct gctgaatgtt catcatcgcg
    27241 tgaagttatt gtatggcgag gggctgcata tccgccgcct ggaaccagga acggaaattg
    27301 cgttttacat tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag
    27361 ccggagtgat attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact
    27421 gagctggcta attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct
    27481 ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat
    27541 tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt
    27601 tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt
    27661 tgactacatt ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga
    27721 agcggcctgg caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa
    27781 tgacaccatt aatctggtga aagatgagcg aataatcgtc acgccaatta acgatatcta
    27841 ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat
    27901 gccgatgaac attaccgaat tttgcagcaa actgccgccg tcgcattttt tccgctgcca
    27961 tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac
    28021 ctacattctg cgactgaaag atctggattt tgaagtgccg gtcagccgca gcaaagtgaa
    28081 agaatttcgc cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc
    28141 cgccgatcaa caatttttcg ccgatctgtt cagcggcctg gtgcttaacc cgcaactact
    28201 ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga
    28261 ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca
    28321 gcagcgaatg gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccgat
    28381 caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc
    28441 gttttacgat agccgcacca cgtcgttgtt gcatcctgct cgccagatcc agcttcccag
    28501 cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc
    28561 gctggagcag aatatcattc agccactggt gttaagtttg ctgcatcttt gccgtaacgt
    28621 ggtgaatatg ccgccgggca attcgcagcc gcgcggcgat tttctctatc acagcatttg
    28681 taactgggtt caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt
    28741 taatatcacg cccaatcatc tgtcaaaact gtttgctcag catggaacga tgcgttttat
    28801 cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct
    28861 gtcaattcat gaagtggcac agcgttgcgg ttttccggat agcgactatt tttgtcgcgt
    28921 tttccggcgt cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg
    28981 tcagaaggtt aattctgttt ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa
    29041 caacgactgg cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac
    29101 atgattcatc ccttcgttag cacccagcgt cagcatgatc accagttcga cttcgcccat
    29161 ttctgactgc cagtcaatcg gtttcgccag ccgggcaatg ctgatactgg aatgacggat
    29221 ccactgagat ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc
    29281 ttcccgctgc cagacatctt cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc
    29341 gaggttgccg cacaggaact ggatcgcctg ctctttattg ctaaaatccc gatcaacaaa
    29401 gatattctcc agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa
    29461 cgcttcaatt tcctgcgcac tgcggcattc acatgcctga cgcgccagtt cccgacacgc
    29521 ctcgctatcc agttgacgaa gctggctttt caccgccgga atacgcgggc tactcatact
    29581 cagctcatcc aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc
    29641 accgcaaatg cctacccatt tgccccgctg atgcgcagtg gtaacaatct gctgcaacat
    29701 gcgcaggaac gatggcgtaa tcgggttata cagcggcgat acgcgcgggt tattacgatc
    29761 gaccgcatac agatactggg tcatatcgtt agaaccgata ctgaagaaat cgacctcatc
    29821 gcagaagtgg tcgatgatgt aacacaccga cggcacttcc accatgatac ccagagtaat
    29881 cgtctctgca tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc
    29941 cttcacccat aagatctgat cgaggctgtg aaccatcggg atcatcaact gggcgttgcc
    30001 aaaactggca gcgcgcaaaa tcgcccgtag ttgagtgcgg aacaggccag caaattccgg
    30061 gtaaatacgt accgcgcgat agccgaggaa cgggttctct tcctggggaa tattcagata
    30121 aggaatgctt ttatcgcctc cgatgtccat tgtgcggaag ataatcggct tgtcgcccgc
    30181 tgccagtagc acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcacg
    30241 atccatatac agcatttcgg tacggaacag cccgacgcct tccgcgccgt tggcaaacgc
    30301 gcctggtgct tccagagcgg tgccaatatt cgccgcgata tcaatacgct tgttatcacg
    30361 ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt
    30421 ctgcgcaacc tgataataac cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca
    30481 ctgggcgtca agcaccgccg gttgcccggc ataacgggca atcgcatcca gcggcaggcc
    30541 gctcagtacc gggatcgccg aggcgcgggc cagaatcagg gtatgcgagg tgcgcccggt
    30601 tttctccaga atcatgcccg caagattttt caaatcaagg ctcaaaaact ggcttggggt
    30661 taaatcttca gccaccagaa tggtcggttt ttccagcacc agattgttgc gcggcttcag
    30721 ttccggccag gtgatatgca gcaactgttc gctaatatcg cgaatgtcgc taacacgttc
    30781 gcgcagataa tcgctggcag aagcagaaag cttggcgcaa acctgctcca tattgctgat
    30841 gatcgccgcc cccagtccct gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa
    30901 ttcatcatcc tgaatcagcg acaaatgggc gctgaggata gttttgcttt cgccgtcacg
    30961 ctcacgcaat tgctggttca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt
    31021 ggaatcttgc acactggcgg ggattgcccg ataactgtcg aggctgtcgc tttgtaacag
    31081 ggtcagcgta cccacgccga cgccgcttgc cagcacattg ccgtacagta aatccgggtt
    31141 aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg taggctggac
    31201 gctgtcgctg tcgataaagc gcacctggat gtactcttcc agcacgcgcc gcgcctgctc
    31261 ttcatcgctg ccgctaatgt tcaggctgca actgtcatta aatagggtgc cggtgccaat
    31321 cagcgccagc gaacttttcg catcggcctt tgcgttctgg cgatggttaa taaaggtgac
    31381 ttcactttgc cactggctgc actgttcttt aagttcccac gccggacgag catgcagacc
    31441 gttaggcaaa ggacagagaa attgaatcgt taacataacc gctccgggtt aatcaggaga
    31501 tcatctgaaa tccgtcagtt gaacaaccgt ctcacgggta agacgttgaa taagggcaga
    31561 taaaagttgc tccatctgca aaatgtcgcg gcaatcggca atcgatgccg cgcaatgtcc
    31621 gtggcgggtg gcaggcccca ttaccactgt gggtacgcca gtaccggtta agtgcaccgc
    31681 tccgccgtcc gtgccgccgt tgctgaacat atctgcctgc aacggtacgc caatttcagc
    31741 cgccacggtt tcgatccagg cggtaagttt tggcggcgca atcagtgact tgtcgcttaa
    31801 caccagcatc gggccgttac caatctggcg atggttagcc gcgccataat caaagttttt
    31861 cgcccagcag gcggtatcca gcacaatggc gacgtccggc gacaccgcgc gggtagcggt
    31921 ttgcccaccg cgtaatccca cctcttcgct ggaacttgcc accagccaca cctccgcagg
    31981 cagttcggca tcgtgcagct cacgtagtaa cgtcaccagc agatagcaac cgaggcgatc
    32041 atcaaaggct ttccccatca cccgctggtg agggagaacc tgaaaagttg tatcaaaggt
    32101 gacgcgatcg cctggacgaa ttcccgcctg catcacttcg tcatacgagc gcgcaccaat
    32161 gtccacgcgc atggcgctga cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat
    32221 tttgcactct tcacgggtgg tgatgcgcac cgctgcagct ggcgggcagc catgcgcacg
    32281 ttgccaaccg gcagcacatc aattgcccct tcgcgggaga tgctacgcac cataaatccc
    32341 acttcgtcca tatgggcgca gatcatcacc ttcggacctg tcgattcatt gaggcggatc
    32401 agcaccgatc ccagaccatc aaatcgcact tctttttgca ggcgatccgc ttcttccagc
    32461 aggatctgcc gcacttcctg ttccgaggag gcgatcgcat ctgcctcgct caacgctttt
    32521 aatagcgata aatccattat gcctctcccg tgagcaacac tgttttcggc atggcgtaga
    32581 gcacttctgc gccttgcggg gtaaccagca caacatcttc gatgcgcacg cccccttgcc
    32641 ctggcaaata aatccccggc tccacggtca gtaacatgcc tggctgtagc gtcgtggtgt
    32701 cccgcggtga aaaacgcgga tcttcatgaa cttcaatgcc gatagcatga ccggtgttat
    32761 gaccgaaata gtggctaaaa cctgcctcgg taatcacttg gcgcgcggca tcgtcaacct
    32821 gctggcagcg cacgccgggg cgaattgcag agattgccgc gagctgtgcc tgcaggacaa
    32881 tctgatagac gtcaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca
    32941 aggtgcgcgt catatcagag cagtagccct gatacagcgc accaaaatcg agagtgacaa
    33001 actcgcccgc tgcaacaatc ttgtcgctgg ctttgccgtg cggcagcgcc ccgcgccagc
    33061 cgctggcgac aatggtgtca aaagaggctt tttctgcgcc ctgctggcgc ataaaccact
    33121 ccagttcagc ggctatctcg cgctcgctca tccccgcctg aataaagcgg cgaatatgct
    33181 ccgctccgcg atcggcaatc ccacaggcga ggcggattat ctccacctcc tctggcgttt
    33241 tgatttgccg cagcacatcc ggcgtggcgc taaccagttt cgcattgagt tcagacttcc
    33301 agcggtgcgc ggtttcccaa ctcacctgct ggccttcaaa accgagcgtt tgcaactgct
    33361 catcggcaat gatttgattg acgatagtgg taagcgtgtt cgtcgcgtca agcaaatgca
    33421 actggtagcc ttgcgcacgg gcttccacct caacgtaata gcgcgaatcc accagaatgt
    33481 gcgcactttc acggctaata accacatagc ctgatccggt ggagatcccc agatgcggct
    33541 gtttgttctg ccgtgaggag agaagcactg catccagttg ttgcgcctta agccagtcgc
    33601 gcagcgaagc gagtaatgtc atctggattt tccttcttac aggctatcga ttaacaattt
    33661 gcctttacgg aacatcatca gacgcaggaa caccaccatc agcgcagtta tgactgcccc
    33721 cagcgcgatc cccgccatat agacgcccag gttagtgacc agcggccatg cccagatagc
    33781 ggactctggg aaccattgca ctgcgcccag ccagacagcg gcggttgagc cgacaatagc
    33841 gccgaccata tacgacggaa tggcggtgat ggggctttcc agcgcaaacg gaatcgcacc
    33901 ctcactgatc cccataaacg ccaggaacat ggcggtctta ccctgcggat aaagttgagc
    33961 gttgaacagg cgtttgccgg ttaaacgacg gtcgataatg gtcgccagcc ccagaccaat
    34021 cggcggaata acgatagcga tagaacgcgc ggtaaccggt aatacgtggt cggtggtaaa
    34081 gctgaaggca acgaaacctg ccgctttgtt gattgggcta ccaaggtcga ttgcggtcgc
    34141 cgcggcaatc cccattgcat acatcagcgc gcccttctca cctgcggcag tcagcacggt
    34201 acggataccg ccgttgatcc agccaccaaa tggggtgatg acatagtaca tcgccagcat
    34261 gacaaaaatg gcggaaagaa tcggtagcaa aaatgtggtt ttgaacgcta acaggaaatc
    34321 tggtaactgg attttctggt tcatccattt cacaaggtaa ccagcaacga tagatataat
    34381 cagcgcacca atgaaggtgg acggcaccgg cgaagaggtc gcccattgca tagtgctggg
    34441 atcgaagttc agcagttgcg tcggctgggt agacatcaag ccgccgataa aaccagccgg
    34501 gaacgccagt ttgccgccga tagagttcgc cacaaaagcg gcaaacatcg gaatagcaaa
    34561 gccaaacaac acgccgccga aggactgcga cagccaggca aatttcagta aggagaggtc
    34621 gaaaccggag aatttcccgc tattaagagc atccatgatg ccgatctcag caggaatttt
    34681 aagccagcta taagcaatca gctggctgaa agcgaggatc acaccgccca taattaaggt
    34741 cggcaccatg cgtgaaatcc ctgacatcac atgctgcggg agttcacccc agaagctgct
    34801 tttagaggcc tgcggattct taactgccgc tgccgcaccg gatgcgccag gcacaacggt
    34861 tgcactgcgt tttttaatgg ccataacaat tatccctgtc gaatattact gttgttcaga
    34921 ggcaatcatc tcttcgattt ctttgatgat tcctacagcg tttttaattg cgtcctgcaa
    34981 agtgatttca taaacgtcgc gtgattcgaa acgttcgtta tcttccgggg taactgccac
    35041 ggaatggatg atgatggtcg cttcggcgat atcctgcgcc gtcaggcgat tctggatgcc
    35101 gtccgcgccc tgggtttcaa ttttcacttc ataaccggct tctaccgccg cttcttccag
    35161 cgcctgagcg gccataaaag tgtgagccag gcccatcggg caggcacata aggcaatcag
    35221 tttcttactc atcgttaatt cctcgttcag tgatagttga cgagtgaaat tatgaacgtg
    35281 caaaacaaat cgccgttacc agacaaataa tgcatttact ggagaattaa tcccgctgta
    35341 tggaagtgtg acgcaggtcg gccttgtgag gggacacaaa gaaaaattga attaactgta
    35401 tgatttaaaa gattatcggg agagttacct cccgatataa caggaaggat ttacagaatg
    35461 tgacctaagg tctggcgtaa atgtgcaccg gaaccgagaa gacccggatt gtcatggacg
    35521 atgagataca ccggaatatc atggacatat tctttaaagc gccctttatc ttcaaatgcg
    35581 gcacggaaac cagaggcttt gaagaactca aggaagcgcg gcacgatacc gcccgcaata
    35641 aacacgccgc caaatgtccc gagattgagc gccagattgc cgccaaaacg gcccataatg
    35701 acgcaaaaca gcgacaacgc gcggcggcaa tcggtgcagc tgtcagccag cgcgcgttcg
    35761 gtaatatctt ttggcttgag attttctggc aggcggttgt cagctttcac aattgcgcga
    35821 tacaaattca ccagcccagg gccagaaagc acgcgctccg ccgaaacatg accaatttcc
    35881 gcacgcaata tttcgaggat aatggcctct tcttcactat tcggcgcaaa atcaacgtga
    35941 ccgccttcgc ccggcaagct tacccaacgc ttatcgacat ggaccagatg cgcaacccca
    36001 agccctgttc cggcaccgta aaccgcaata ggtttacctt ctaccggttc tgcgccacca
    36061 aactgaatca gatgctcttt tttcagcatc gggatcgcca tcgatacagc ggtaaaatcg
    36121 ttaataattt ccagatggct aaaaccgaga ttctttttca tttcggcaat tgagaacgcc
    36181 caggtatggt tggtcattgc cacccagtca ccggtaatcg ggcaagcgat ggcaatacag
    36241 ccgtctttca cctcgacctt atgttcttca agataaacgc gaatgaccgc ttcgaggctg
    36301 gggtaatcaa gccctgaata ggtcttagcc tgcgagattt caccactggc aatatcacac
    36361 agagcaagac gtgcgttggt gccgcccaca tcaccgacta atgcatactt tgtcattctt
    36421 caactgctcc gctaaagtca aaataattct ttctcacact gtaaatacct gggggcataa
    36481 caacaacgcc ggaaaggcag gctccctgta aatatcgatc tgggtcacac aattacttta
    36541 tcgtttcagc accaattgca gcgatgcctt tttgcaagct gggcaaacta agtatctgac
    36601 cccgcataag gaatagaaca tgctccatcc gcgagccaga accatgttgt tattatcgct
    36661 ccccgccgtg gcaattggga ttgcgtccag tcttattctg attgtggtga tgaaaatcgc
    36721 ctcggtatta cagaatttgc tctggcaacg actgccggga actctgggga tagcccagga
    36781 ttcacctttc tggatcatcg gtgtattaac gctaacaggt attgcggtgg ggttggttat
    36841 ccgcttcagc cagggtcatg ccggaccaga ccccgcctgt gaaccgctga tcggcgcacc
    36901 ggttccacct tccgcgctac caggacttat cgtagcatta attctcggtc ttgctggcgg
    36961 cgtcagcctg gggccagaac atccgatcat gaccgtcaat atcgcccttg ccgtcgccat
    37021 tggcgctcgt ctgttaccgc gcgtcaaccg aatggagtgg actattttag cctctgccgg
    37081 aaccatcggc gcgctgtttg gtacacctgt tgcggcggcg ttgatatttt cgcaaacctt
    37141 aaatggcagt agtgaagttc cgctatggga tcgtcttttt gcgccattaa tggcggcagc
    37201 agctggtgca cttactaccg gattattttt ccatccccat ttttcactgc ccattgcgca
    37261 ttacggacag atggaaatga ccgatattct cagcggtgca attgtcgcgg cgattgccat
    37321 cgcagcagga atggtcgccg tatggtgctt accacggttg cacgcgatga tgaatcaaat
    37381 gaaaaatccg gtgctcgtgc tgggtattgg cggatttatt ctcggtattc tgggggttat
    37441 tggtggacca gtttcgctgt ttaaagggct ggatgagatg cagcagatgg tggcaaatca
    37501 ggctttcagc accagcgatt actttttgct ggcggtaatt aaacttgccg ccctggtcgt
    37561 tgctgccgcc agtggctttc gcggtgggcg aatcttcccg gcagtgtttg tcggcgtggc
    37621 attagggttg atgctgcatg agcacgtacc cgccgtacca gcggcaataa ccgtttcttg
    37681 cgctattctc ggcatcgtgc tggtggtaac acgcgatggc tggttaagtc tttttatggc
    37741 ggcggtcgtt gtacccaata ccacattgct accgctgctc tgtatcgtca tgctcccggc
    37801 atggctgtta ttagcaggta agccgatgat gatggtcaat cgtccgaagc aacagccacc
    37861 ccacgataac gtttagcaaa aaatgctctc ctttatgatt taagagttat ggcgatgata
    37921 cggggcttta cctccccgta atattgcgtt aacaggccgc tgacggcttc gatcgtgaag
    37981 gagaataaca atgttcaggt cactgtttct ggcggccgcc ctgatggcat ttaccccgct
    38041 tgcagcaaac gcaggtgaaa tcaccctact gccatcaatc aaattacaaa ttggcgatcg
    38101 cgatcattac ggtaattact gggacggtgg tcactggcgc gaccgtgact actggcatcg
    38161 caattatgag tggcgtaaaa accgctggtg gcgtcatgat aatggctatc atcgtggctg
    38221 ggataagcgt aaagcgtatg agcgtggcta tcgtgaaggc tggcgcgatc gtgacgatca
    38281 tcgcggaaaa ggccgcggac atggacaccg ccattaaaca cattcgccaa tggaacacaa
    38341 tgcctgatgc gacgctagcg cgtcttatca ggcctacaaa ccggctcttt aacgcgtagg
    38401 gcggataagg cgtttacgcc gcatccgcca atagtgccag atgcgacgct cattcaacta
    38461 caatcccagc gccgtcccca ccaacaacca gatattcagc gccacgacca gcactacaat
    38521 cacccagcct gtctgtttta cgcgtttgct gttcaccaga tcgcccatca acttgctgtc
    38581 actggtgaaa atcagcagtg gaaccagcgc cagggcgata ccaaaactta acagcacctg
    38641 actcataacc agaatccgtg tcggatctaa tcccatcaga atgacaataa atgacggcaa
    38701 catggtgact gtacgacgca cccacagcgg gatatggaag cgaatgaatc cctgcatcac
    38761 cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt ccggcagcaa ccagacttaa
    38821 tccaaagacc gttgccgcag catggcttaa cagcggttgc agcgtcagat aagcctcatc
    38881 aagatcggca acaccagtat gaccagaaaa gtggaacgcc gccgcagctg tagccatcat
    38941 cgccagattg acaaaaccgg caatcgtcat ggcgatagcc acatcccatt tggtggcgga
    39001 ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg agcgaagagt gcaaataaat
    39061 cacatgcggc ataatcgtcg cccctaacac gcctgctgcc aggaagaccg cctccgaagt
    39121 aggtaaactc gggatcacca ttcctttacc caactgcgcc aggttaggct gggagaaaat
    39181 caactcgaca atgtaagccg cggcaacaaa caacagtaac ccgccaatca ctttctccag
    39241 cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa gtcgcgatcc ccgtcagcac
    39301 cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa ccgatcgccg caccaataaa
    39361 ttccgccagg tcggttgcca tcgcaataat ttctgcctga acccaataga accacactac
    39421 gggacgcgga tagtgatcac gaatctgctc cgccagattt ttaccggtgg caatccctag
    39481 tttggcagag aggatctgaa tcagcatcgc catcaggttg gcccaaacga caacccacag
    39541 tagctgatag ccgaagctag cacccgcctg aatattggtc gcaaagttac cgggatcgat
    39601 ataaccaatc gccgcaatga acgcaggtcc cattaatgcg agcctcatct tgcgcgccgc
    39661 ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg tgcctctaaa acatagcctt
    39721 tgctatgttt catgctatgc caaacgagaa tgattatcaa attcatttga atgggttgtg
    39781 gtgatttctt tgatagacca ggattatgac tacgaaaaga ttgatgattt gaatgttatg
    39841 gttatgttta atgttagcac atttacataa ctttcagctt ccatacacaa catagcagaa
    39901 atgtatgaca gatcactatt tttgaagctt gtcacaggac gtcattatag tgtgtgtcag
    39961 atctcgtttt ccttaaccat gttacataga atgtgcacgg aaatttaacc tgcctcatat
    40021 ttggagcaaa tatggaccgc gtccttcatt ttgtactggc acttgccgtt gttgcgattc
    40081 tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat ccgttatgtt attcaactgc
    40141 ttgttatcga agtgttactg gcgtggttct tcctgaactc cgacgttggt ttaggcttcg
    40201 tgaaaggctt ctccgaaatg ttcgaaaaac tgctcggatt tgccaacgaa gggactaact
    40261 tcgtctttgg tagcatgaat gatcaaggcc tggcattctt cttcctgaaa gtgctgtgcc
    40321 caatcgtctt tatctctgca ctgatcggta ttctccagca cattcgcgtg ttgccggtta
    40381 ttatccgcgc aattggtttc ctgctctcca aagtcaacgg tatgggcaaa ctggaatcct
    40441 ttaacgccgt cagctccctg attctgggtc agtctgaaaa ctttattgcc tataaagata
    40501 tcctcggcaa aatctcccgt aatcgtatgt acaccatggc tgccacggca atgtccaccg
    40561 tgtcgatgtc catcgttggt gcatacatga ccatgctgga accgaaatac gtcgttgctg
    40621 cgctggtact gaacatgttc agcaccttta tcgtgctgtc gctgatcaat ccttaccgtg
    40681 ttgatgccag tgaagaaaac atccagatgt ccaacctgca cgaaggtcag agcttcttcg
    40741 aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc cattatcgtt gccgcgatgc
    40801 tgatcggctt tatcgccctg atcgccgcgc tgaacgcact gtttgccacc gttactggct
    40861 ggtttggcta cagcatctcc ttccagggca tcctgggcta catcttctat ccgattgcat
    40921 gggtgatggg tgttccttcc agtgaagcac tgcaagtggg cagtatcatg gcgaccaaac
    40981 tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa aattgcttcc acgctctctc
    41041 cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt cgctaacttc tcttcaatcg
    41101 ggattatcgc aggtgcagtt aaaggcctga atgaagagca aggtaacgtg gtttctcgct
    41161 tcggtctgaa gctggtttac ggctctaccc tggtgagtgt gctgtctgcg tcaatcgcag
    41221 cactggtgct gtaagaccat acataaaaaa gccggggata attccccggt ttttttacgc
    41281 ctgttaatca gctaatggct gcgggcgacc aatcaaatac ccttgcagat attgcacccc
    41341 gagcttatgc aatagcgcct gctgctgttg cgtctcgaca aactccgcga ccacactcaa
    41401 tgacttcgct ttcgccagat cggtaattga tctcacaatc atcgcatcca gcgtgttcgt
    41461 gacaatatct ttcacaaaga cgccatcaat tttgatgata tcagcctgca aacgctttaa
    41521 ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt
    41581 atgcagctgc tcgatgttgt acatgctgct ttctgcgtta gaaaacgcct gctcctcggt
    41641 gatctcaaga atgaccgcct gcggggagat gtgataacgc ttaaacagac gaataatccg
    41701 cccggcaata ttcttttgca gcagcgtgag cggcattaaa ttgactgaaa agcgcggtcc
    41761 ttttttgtcg caagggtgtg ttgccagcca cttcaacaag gattccagca cttgcaaatc
    41821 aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga aacttatccg gggtcataat
    41881 gccgccgtca tatttcagtc gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg
    41941 aattggctgg gcgtagagca gcaaatcacc ctgatctaac gccgtgcgaa tggtattcag
    42001 caatagcacc tgtttagtgg tctgcccgga aaccatctcc tctctgctat ccagcgccag
    42061 cacatgatga tgtgcgcagg attgctccgc cagccagctt aactgcccca acaagggttg
    42121 cagggtttcc tgatttccat caaaacgccc ccaggcagca ccatagccca tatccagccc
    42181 ggtattgttc cagtgaattt gccgactatt caggatgtta atcatatgct ggagtcgccc
    42241 ttccgtttcc ggcccgctca gcactaacag cagttcacta cccggcaatt gatacaactt
    42301 ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt gagcggatac agtgaacgcg
    42361 catcattaag ccgtaatgac gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa
    42421 actcttgccc gcctcttgtt ccggcgcttg ttccaacgca cgaaagttgg gtaggagcgt
    42481 taacggatct gtcagcgcct gcaaatgcca gcggcgatta agccattcac tgcgatgata
    42541 aatgcgcacc atatagagca ggcaaacgct aaaggaaatc agcaccgcaa gaataaatgc
    42601 cagcgaatat tcggtttccc ccccttgcaa aaagttctgg ttgtaattca gaaggcaaag
    42661 cgttgaaaca gcccaggtga gatttaaaaa cggatagcga agcttaccga ccccgagggt
    42721 aaagatgatg aagaaaacgg gtaccaggta accggcaata aagtcgtttt cataaggtgt
    42781 gcacaacaga agtagaagca cgcttaacgc tgccagccag cttaaggtaa atgcgcgttt
    42841 ctctttgccc aacgacggcg cgatatccct gcgccacaat atctgcgcaa agtggggact
    42901 tacaatcatg cgagtgagat agtagaaaag catgttgtaa atcagcacag cggtgaaaag
    42961 gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg tggatatctt
    43021 tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc cgatcggcgt
    43081 caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc cataacgcca
    43141 acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa acgtctggct
    43201 accgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat tggtgcaaaa
    43261 catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga ttgacagcat
    43321 tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg caagtggcga
    43381 aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg tgaatatctt
    43441 gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac tgatcggggt
    43501 tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg ccagcctgtt
    43561 gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg tagactgtat
    43621 tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tgcagcgagg gttggaaatt
    43681 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    43741 gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat catataaatt
    43801 ccgcccgaat gtgtcaacgg caaattgcaa cgtgtaattt caatcgctga aaaatcaggc
    43861 aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt
    43921 aaagtggtgt tataaaatga actactaata gacccacata cattcaggga attgttatgt
    43981 tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca
    44041 ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc gtccagggtg
    44101 gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac
    44161 gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc
    44221 tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc
    44281 tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg
    44341 aaaagattac gcaacaaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa
    44401 caaactgtta accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg
    44461 aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac
    44521 acgccagcct tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac
    44581 gctcctggag cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca
    44641 gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc
    44701 gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc
    44761 gtttatcttt ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc
    44821 tttgttgata cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc
    44881 tggagactgc cccgcaccgg ttacggcgac acgcagcggc ataccaactt tacccatacc
    44941 cacttccagc tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc
    45001 agtaatcgcg gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag
    45061 atgttttttc gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct
    45121 ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag
    45181 ctgcgggccg ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc
    45241 aacatactcc ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt
    45301 gttgaacgca ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc
    45361 acgagtgaag atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag
    45421 tgcttctggc aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt
    45481 ggacagtttt ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg
    45541 cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc
    45601 acggataacg tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt
    45661 tggggaacca tcggtacggc ggataatcag atcatccagc tcctggttgc tgaactcgat
    45721 cggaccacgg atctgatcgt caaaaacaac agaaccttcc tgcggattag caaaacgcac
    45781 gacacacggt tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg
    45841 cggcttctca cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca
    45901 atagcattta taagcagtgc cctcttccag catctgatcg atcaccgcat tgtagcgatc
    45961 aaaacgtttg gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc
    46021 atccataatg gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg
    46081 cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc
    46141 gccgccaacg tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa
    46201 atggccttac gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg
    46261 atattctatc actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat
    46321 tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac
    46381 gacgaacgaa caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc
    46441 gactccacac agcg
//
LOCUS       DAHBHC010000034        45002 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHBHC010000034 DAHBHC010000000
VERSION     DAHBHC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.034"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(388..918)
                     /locus_tag="M2A35_002310"
     CDS             complement(388..918)
                     /locus_tag="M2A35_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCB7198603.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1261..1911)
                     /gene="zinT"
                     /locus_tag="M2A35_002311"
     CDS             complement(1261..1911)
                     /gene="zinT"
                     /locus_tag="M2A35_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCB7198604.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2168..2803)
                     /gene="msrQ"
                     /locus_tag="M2A35_002312"
     CDS             complement(2168..2803)
                     /gene="msrQ"
                     /locus_tag="M2A35_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCB7198605.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(2804..3808)
                     /gene="msrP"
                     /locus_tag="M2A35_002313"
     CDS             complement(2804..3808)
                     /gene="msrP"
                     /locus_tag="M2A35_002313"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCB7198606.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3917..4330)
                     /gene="hiuH"
                     /locus_tag="M2A35_002314"
     CDS             complement(3917..4330)
                     /gene="hiuH"
                     /locus_tag="M2A35_002314"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCB7198607.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4463..5134
                     /gene="hprR"
                     /locus_tag="M2A35_002315"
     CDS             4463..5134
                     /gene="hprR"
                     /locus_tag="M2A35_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCB7198608.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5134..6492
                     /gene="hprS"
                     /locus_tag="M2A35_002316"
     CDS             5134..6492
                     /gene="hprS"
                     /locus_tag="M2A35_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCB7198609.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6599..7450)
                     /gene="hchA"
                     /locus_tag="M2A35_002317"
     CDS             complement(6599..7450)
                     /gene="hchA"
                     /locus_tag="M2A35_002317"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCB7198610.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8043..9116)
                     /locus_tag="M2A35_002318"
     CDS             complement(8043..9116)
                     /locus_tag="M2A35_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063070036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCB7198611.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
     gene            9684..10049
                     /locus_tag="M2A35_002319"
     CDS             9684..10049
                     /locus_tag="M2A35_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCB7198612.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            10089..10784
                     /locus_tag="M2A35_002320"
     CDS             10089..10784
                     /locus_tag="M2A35_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCB7198613.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            10851..12269
                     /gene="dcm"
                     /locus_tag="M2A35_002321"
     CDS             10851..12269
                     /gene="dcm"
                     /locus_tag="M2A35_002321"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCB7198614.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSATILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTEGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQCVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            12250..12720
                     /gene="vsr"
                     /locus_tag="M2A35_002322"
     CDS             12250..12720
                     /gene="vsr"
                     /locus_tag="M2A35_002322"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCB7198615.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYCCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(12709..13629)
                     /gene="yedA"
                     /locus_tag="M2A35_002323"
     CDS             complement(12709..13629)
                     /gene="yedA"
                     /locus_tag="M2A35_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCB7198616.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGVGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            13802..14719
                     /locus_tag="M2A35_002324"
     CDS             13802..14719
                     /locus_tag="M2A35_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCB7198617.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            14798..14980
                     /locus_tag="M2A35_002325"
     CDS             14798..14980
                     /locus_tag="M2A35_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCB7198618.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            15151..16845
                     /gene="yedQ"
                     /locus_tag="M2A35_002326"
     CDS             15151..16845
                     /gene="yedQ"
                     /locus_tag="M2A35_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCB7198619.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(16842..17657)
                     /locus_tag="M2A35_002327"
     CDS             complement(16842..17657)
                     /locus_tag="M2A35_002327"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCB7198620.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(17955..18182)
                     /gene="yodD"
                     /locus_tag="M2A35_002328"
     CDS             complement(17955..18182)
                     /gene="yodD"
                     /locus_tag="M2A35_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCB7198621.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18345..18533
                     /gene="dsrB"
                     /locus_tag="M2A35_002329"
     CDS             18345..18533
                     /gene="dsrB"
                     /locus_tag="M2A35_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCB7198622.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(18577..19200)
                     /gene="rcsA"
                     /locus_tag="M2A35_002330"
     CDS             complement(18577..19200)
                     /gene="rcsA"
                     /locus_tag="M2A35_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCB7198623.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(19490..20275)
                     /gene="fliR"
                     /locus_tag="M2A35_002331"
     CDS             complement(19490..20275)
                     /gene="fliR"
                     /locus_tag="M2A35_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCB7198624.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(20284..20553)
                     /gene="fliQ"
                     /locus_tag="M2A35_002332"
     CDS             complement(20284..20553)
                     /gene="fliQ"
                     /locus_tag="M2A35_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCB7198625.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(20563..21300)
                     /gene="fliP"
                     /locus_tag="M2A35_002333"
     CDS             complement(20563..21300)
                     /gene="fliP"
                     /locus_tag="M2A35_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCB7198626.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEAQEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(21300..21665)
                     /gene="fliO"
                     /locus_tag="M2A35_002334"
     CDS             complement(21300..21665)
                     /gene="fliO"
                     /locus_tag="M2A35_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCB7198627.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            complement(21668..22081)
                     /gene="fliN"
                     /locus_tag="M2A35_002335"
     CDS             complement(21668..22081)
                     /gene="fliN"
                     /locus_tag="M2A35_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCB7198628.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(22078..23081)
                     /gene="fliM"
                     /locus_tag="M2A35_002336"
                     /pseudo
     CDS             complement(22078..23081)
                     /gene="fliM"
                     /locus_tag="M2A35_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
     gene            complement(23086..23550)
                     /gene="fliL"
                     /locus_tag="M2A35_002337"
     CDS             complement(23086..23550)
                     /gene="fliL"
                     /locus_tag="M2A35_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCB7198629.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(23655..24782)
                     /gene="fliK"
                     /locus_tag="M2A35_002338"
     CDS             complement(23655..24782)
                     /gene="fliK"
                     /locus_tag="M2A35_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCB7198630.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            complement(24779..25222)
                     /gene="fliJ"
                     /locus_tag="M2A35_002339"
     CDS             complement(24779..25222)
                     /gene="fliJ"
                     /locus_tag="M2A35_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCB7198631.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(25241..26614)
                     /gene="fliI"
                     /locus_tag="M2A35_002340"
     CDS             complement(25241..26614)
                     /gene="fliI"
                     /locus_tag="M2A35_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCB7198632.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(26614..27300)
                     /gene="fliH"
                     /locus_tag="M2A35_002341"
     CDS             complement(26614..27300)
                     /gene="fliH"
                     /locus_tag="M2A35_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCB7198633.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLL
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(27293..28288)
                     /gene="fliG"
                     /locus_tag="M2A35_002342"
     CDS             complement(27293..28288)
                     /gene="fliG"
                     /locus_tag="M2A35_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCB7198634.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(28281..29939)
                     /gene="fliF"
                     /locus_tag="M2A35_002343"
     CDS             complement(28281..29939)
                     /gene="fliF"
                     /locus_tag="M2A35_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCB7198635.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            30154..30468
                     /gene="fliE"
                     /locus_tag="M2A35_002344"
     CDS             30154..30468
                     /gene="fliE"
                     /locus_tag="M2A35_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCB7198636.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(30861..31529)
                     /locus_tag="M2A35_002345"
     CDS             complement(30861..31529)
                     /locus_tag="M2A35_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCB7198637.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(31638..31871)
                     /gene="yedF"
                     /locus_tag="M2A35_002346"
     CDS             complement(31638..31871)
                     /gene="yedF"
                     /locus_tag="M2A35_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCB7198638.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(31868..33073)
                     /gene="yedE"
                     /locus_tag="M2A35_002347"
     CDS             complement(31868..33073)
                     /gene="yedE"
                     /locus_tag="M2A35_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCB7198639.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            33260..33673
                     /locus_tag="M2A35_002348"
     CDS             33260..33673
                     /locus_tag="M2A35_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7198640.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(33707..35194)
                     /gene="amyA"
                     /locus_tag="M2A35_002349"
     CDS             complement(33707..35194)
                     /gene="amyA"
                     /locus_tag="M2A35_002349"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCB7198641.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(35272..35637)
                     /gene="fliT"
                     /locus_tag="M2A35_002350"
     CDS             complement(35272..35637)
                     /gene="fliT"
                     /locus_tag="M2A35_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCB7198642.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            complement(35637..36047)
                     /gene="fliS"
                     /locus_tag="M2A35_002351"
     CDS             complement(35637..36047)
                     /gene="fliS"
                     /locus_tag="M2A35_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCB7198643.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(36062..37474)
                     /gene="fliD"
                     /locus_tag="M2A35_002352"
     CDS             complement(36062..37474)
                     /gene="fliD"
                     /locus_tag="M2A35_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCB7198644.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            37729..39222
                     /locus_tag="M2A35_002353"
     CDS             37729..39222
                     /locus_tag="M2A35_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="structural flagella protein; individual Salmonella
                     serotypes usually alternate between the production of 2
                     antigenic forms of flagella, termed phase 1 and phase 2,
                     each specified by separate structural genes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCB7198645.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            39387..40106
                     /gene="fliA"
                     /locus_tag="M2A35_002354"
     CDS             39387..40106
                     /gene="fliA"
                     /locus_tag="M2A35_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCB7198646.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            40152..40703
                     /gene="fliZ"
                     /locus_tag="M2A35_002355"
     CDS             40152..40703
                     /gene="fliZ"
                     /locus_tag="M2A35_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCB7198647.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTAREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            40791..41591
                     /gene="tcyJ"
                     /locus_tag="M2A35_002356"
     CDS             40791..41591
                     /gene="tcyJ"
                     /locus_tag="M2A35_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7198648.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            41696..42682
                     /gene="dcyD"
                     /locus_tag="M2A35_002357"
     CDS             41696..42682
                     /gene="dcyD"
                     /locus_tag="M2A35_002357"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCB7198649.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            42697..43365
                     /gene="tcyL"
                     /locus_tag="M2A35_002358"
     CDS             42697..43365
                     /gene="tcyL"
                     /locus_tag="M2A35_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCB7198650.1"
                     /translation="MQESIQLVIDSLPFLLKGAEYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            43362..44114
                     /gene="yecC"
                     /locus_tag="M2A35_002359"
     CDS             43362..44114
                     /gene="yecC"
                     /locus_tag="M2A35_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCB7198651.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            44344..>45002
                     /gene="sdiA"
                     /locus_tag="M2A35_002360"
     CDS             44344..>45002
                     /gene="sdiA"
                     /locus_tag="M2A35_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCB7198652.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     Q"
BASE COUNT        11312 a        10874 c        11378 g        11438 t
ORIGIN      
        1 tcttatcgct caccattatt cttttagaca ttcatcctat gaaactgccg caaagttggt
       61 ggtgggaact gaagttgcgt agagaagggg tcaataattg agtcaaaaaa ggaacgcggc
      121 cgtcagatta aagccgcggg acaaagtcca taatttccaa gagttgttga atttcccagc
      181 ttttttgaaa gcctttgtat catactaaca agtaaaaaag gtttaaatac caaaattaac
      241 ctgtattttt gataagtctt accattgtac gatttacaca gtttctgctc ctccttgttg
      301 atagtggaaa gggtgcattg tgcacttgcg taaacattta tgacgcgttg atgtccatgg
      361 ttcacatttc cttgtcaggg gatcgactta tttgcggcga ggcatcattc gtaacagagt
      421 attatctttc tgaatgtagt gatgaaagag tgctgcgcca gcgtgagctg cgattaaaaa
      481 gtaacctata tttgcccagg tttcgtgaat attttttatc agtgctttaa tttcgctgtt
      541 tggggtaaca aagggagaca cattgaaacc aaggaaactc cacgattttc cactgtaagc
      601 catcaaagca atccccagca gaggaagtgc aaggaaggtt atataaagca tgatgtgcat
      661 tagcgaagcc gctttcattt gccaggcagg tggcggtggc acaatagaag gatcactata
      721 acggtgtttt ataatcaggc gtgaaaacat taacacccaa acgaatatac ccgcattgta
      781 atgtgtttct cgcatcagca gataagtact actaccttta ggaaaccagc cacggagttc
      841 catcgcggca taggttattg caacgaaaag cagcgttatc caatgtaaat aaatttgagt
      901 ttttgagaat cggttcattt ttgtagctcc tgtaattttt acagtcaagc tatcccacga
      961 accttaaaaa atccttatac agatgtggtt ttaagaattt gttatttaaa aaattgatag
     1021 tgcacattgt aaccagccaa tataagcaat ggcgtgagca caggtatctt tctctgttgg
     1081 ccgtattgtg tatggaatcc gttattggtt aactcaatgc gggttagctt attaatatac
     1141 agacacgtca tagtgatgag cgtgtctgta gctagcttgc gttcagtggc gtttgtggat
     1201 ggaccagtgg cagttatgat gacttactaa agcggcatcg aggcgttatc atgagaatac
     1261 tcaatgagac atcatttcct cgaccacttc ttcgctactc aactgatatg gataatacgt
     1321 tggccagttt tccatttcat ttaataaaga ttgctgtgaa tcattaccca taaaaatgtg
     1381 aaaatgagat gattttcgtg gtgcaattat atgatcgcta aattgtatat atttaggggc
     1441 tttgctttca ggatctttac attcgaataa gtaacgaacg cctttcttgc ctgatttata
     1501 ggtgagtatt ttgtatccat cgtaatcata tttacaggat gttgtttcat tatttctatg
     1561 gaattcaaca atgccgtcct caatgccaat catctcgata tctgttgcat aacctttgtg
     1621 ataataatct ttaatttcag caaatgtttt agttttatct gcatccgctt tcttctgaaa
     1681 gacggggtca agtttgccac tttgcagtaa aggataaacg gattgccaga ctccatccca
     1741 gtcactgagc gttcggtttt gtacattggc atcatcaaaa acaccattag cagctttttg
     1801 ttcgacctct gttaagggtt tgccgtgtga gtgatgacca tgcgaaaagg caggagcgct
     1861 aacaataaag acacctaaag caacagccag tttgtgaaga cgaatcgcca aaacagtttc
     1921 ctccagagtc attgtatatg atgtgtaatg ttatattgta acatatggca aaatgcaaca
     1981 actcttagga gatgagagca tagttttctc aggtgtaatt atctatctta ttgaaataaa
     2041 atgaattata ttgataattt gaggttgatg acaaacgaaa cataacctga tgtgatctgc
     2101 gtaggccgga tagggcgctc gcgccgcatc cggcagggat aacgcgtact ttgtcggcaa
     2161 tatgaagtta tacagataat ttgttgtgaa cctgcttgcg taaccggtta aaaagggaac
     2221 gcaacttctt ataccgtaag gctaaaagca gtacagccag cccagcgtag atgagcggtt
     2281 gcggtgaaat aatcttcact gaccacagat aatgtattgg ggccaggatc gcgacaagat
     2341 agacgaaatt atgcaactgt tgccagtgct tgccgagttt tcgctgcatc gcctgggttg
     2401 aagtaaacgc taaagcaagc aaaataaccc agctgataat tcctaacgtt aaataaggcc
     2461 gggtaattaa ctccttgcct aataacgcca gattattcac gcccagctcc agcaatgcgt
     2521 aactggttaa atgcagtgtc gcccaggcaa agcaccataa gcctaacagg cggcgagtgc
     2581 gtatcaataa cggctgtttt gcgtagcgtg ccagaggtgt gattaacaag gtcgccagca
     2641 aaaatttcag tgcagtgcga ccggtaaaat gctgaatatc tttcaccgga tcggcaccca
     2701 gtccaccgtg attgatcgcc cagaccagcc agagaaacgg caacaatccg gcaagatgca
     2761 ggctaacttt cagccatgtc acctgttttg ctgtcagacg cattcagaaa ttctcccgca
     2821 aatccaggcc acgatacagc gatgccacct ggtcggcgta accattaaac agtagcgttg
     2881 gctggcgctg aacatcgagg atgccgcctg aaccaataaa tcgttcggta gcctgtgacc
     2941 agcgcgggtg atcaacatgc ggattaacgt tggcgtaaaa accgtattcg tcaggcgctg
     3001 ccagattcca ggtggttggc ggacgctcgc gggtcagctt aatactgact atcgatttaa
     3061 tccctttaaa gccatatttc cacggtacaa tcagtcgtac cggcgcgcca ttttgtggcg
     3121 gtaacgcctt gccataaaca cccacggtca tcagtgtgag cggatgcatt gcttcgtcga
     3181 gacgcaatcc ttcgacataa ggatatttca gcccgccgcc gataaagcgg tcctgctggc
     3241 ctggcatttg ttccggtgca taaattgttt cgaaagcgac atacttcgca ttgctggtgg
     3301 gttcggcaag cgccagcaat ttgtgcagcg gaaaaccaat ccacggcacc accatcgacc
     3361 atgcctccac gcagcgcata cgataaatac gctcttccag cgggaagcga cgggttaaat
     3421 catcgtgatc gagggtcaat ggttttgcca cttcgccgct gattttcagt gtccatggat
     3481 cggttttcag gctaccagca ttagcggcgg gatcggcttt atccagcccg aattcataga
     3541 agttgttata accggagact ttatctactg gcgtcagtgg caggttattt tgccaggcgg
     3601 caggcttgct gaactccagc ggttttccgg cgggggcggg cgggcgatcg ttccctttaa
     3661 accagctaag cagatcggca tgcgcagcgt gaggcaaaga aagtgcagct gcgctgatgc
     3721 ccagtgcttt taacacctgt cgacgcttca taaagaatac cgactcggcc gtaacatctg
     3781 attcttttaa aaattgattc tttttcataa gccatcacac ttttttgtcg ttagccagca
     3841 agagccgttt actgttaatc tgtcagggag tgtcctccct aacagtagca gagatctgct
     3901 tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata
     3961 aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga
     4021 agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat
     4081 cgccagtagt cgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt
     4141 tggcggtatt aagttgtaac cagccgttgt ccgctttctt ttcaagagtg actgtcacgt
     4201 cggcagcggg ttttccggtt tgctggttca aaatgtgcac gctaagaatg ttttgttgtg
     4261 cggcataaac caaagaaggt aatgaaaatg ctgccgttac tacggagagt actaaataac
     4321 gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcggc
     4381 cagggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt ctataacata
     4441 tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca
     4501 ggagtgggta acgcaggggc tttccgaagc gggttatgtc attgatgccg tttctgatgg
     4561 cagagatggg ctttatcttg cgctgaagga tgattatgca ttgatcattc tggatattat
     4621 gcttccgggt atggatggct ggcagatctt acaaacgtta agaacagcaa agcaaacccc
     4681 tgttatttgc cttactgcaa gggattctgt cgatgacaga gtcagagggc tggacagtgg
     4741 agcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc
     4801 acaattaagg caacatcacg ctttgaattc aacattagaa atcagtggct taagaatgga
     4861 ctctgtaagt caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt
     4921 tcagttactt tggctactgg cctccagagc tggcgaaatt atacccagaa cggttattgc
     4981 gagtgaaatt tggggaatca actttgatag tgataccaat acggtggatg tcgccattcg
     5041 caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaatcgcca caatccgggg
     5101 gatgggctat tcattcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt
     5161 ttaaccttgc tttttatatt gctgctgtct gttgctggcg ctggaattgt ctggactctc
     5221 tataatggcc tggcaagtga gttgaaatgg cgcgatgata caacactcat taaccggaca
     5281 gcacagatca agcagttgtt aattgatggg gtaaatccag atacgttacc tgtatacttt
     5341 aaccggatga tggatgttag tcaggatatc ttgatcattc atggtgatag catcaataaa
     5401 attgttaacc ggacaaatgt cagtgatgac atgttaaata acatacctgc tagtgagaca
     5461 atcagcgcag ctggcattta cagaagcatt attaatgata ctgagattga tgctttacga
     5521 attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc
     5581 agacataaca tgcttgaaca gtataaaatt aatagcatta taatttgcat tgtcgccatt
     5641 gtactttgct cagtattaag tccgctgtta atcagaacgg gattacgaga gatcaaaaag
     5701 ttgagtggtg taacggaagc gctgaattat aacgatagcc gggagcctgt tgaggttagc
     5761 gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca tcaagcctta
     5821 gtcaaagatt ttgaacgcct aagtcaattt gctgacgatc tcgctcatga acttagaacg
     5881 cccattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct
     5941 gaatatcaaa aaacaattgc cggtaacatt gaagagctgg aaaatatttc gcggttaaca
     6001 gagaacatac tgtttcttgc ccgggcagat aaaaacaatg ttttggtgaa actggactcg
     6061 ctttctctca ataaggaagt cgaaaatttg ttggattatc ttgaatacct ttcagacgag
     6121 aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttactg
     6181 caacgaatgt tatcgaatct tattgttaat gccattagat attcaccaga aaaatcgcat
     6241 attcatataa ccagttttct tgataccaac ggctatctta atattgatgt cgccagtcct
     6301 ggaacgaaaa ttcatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg
     6361 cgtcattccg taggtcaggg actaggcctt tctttagtca aagcgattgc cgaattacat
     6421 gggggaagtg ctacgtatca ctatctcaat aagcataatg tgttccggat tacgttaccg
     6481 caaagaaatt aacataaggc ttcagtacgc cataaattaa ccgtgaaaaa acgcagccac
     6541 gtcgtgagta ctaacgcggc tgcgattgat tagcgcttac attcaaacgt aacagggatt
     6601 aacccgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     6661 aaggactgtc gccggtgaga actttacggt ccttatgtac tcgcccggtg atgtcgtcat
     6721 taataatgtt catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agatgacccg
     6781 gcatatagcc tatctctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatggaat
     6841 aaccattcag tgggttatcg ccgtggcgaa gcgccagaaa agccgccggg ccgtggcaaa
     6901 gggagataac aaaacggtca tttttgattg cccactgcaa agcagcagct acgtcctggc
     6961 tttctggtaa accaataagt gcgccatgac caccaggaac aaagattgct gcatattcgc
     7021 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattgcgg aacaacgatt
     7081 tatgttgctc aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa
     7141 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     7201 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     7261 cggttggcaa ataacgttcg tccgcggcga tcaccagaat tttatgctta ccgcgatacg
     7321 gttttggata gtccacgcca tcaagatcag agacgggact ggtatattgg ctaagcgaat
     7381 attctgaagg gaagaatgca ttatcttcag caatatcgac ctgcggattt ttacttgttt
     7441 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     7501 gagctggtcg ctatttttaa ccagtctgga aaattgagtc aatatatcga aagcggtagt
     7561 cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     7621 ttttccttat tgagcaacgt cgtcgcaagt ggcctcaatg attttagacc attatcaaag
     7681 gtacggggcg gcggggagag atttagtgct gtgaaatatt gtggtactga tgaatattta
     7741 tatcgcggat tggtagagaa aagattattt catgcactat ttttgcaaat atttctatat
     7801 gggaagcgtg tttgttacgg ggtaattcat cagaatgtgc atttggggtg ttggctggaa
     7861 gagtcgctgg tagatgttat tgtggcagga ggggcagggt aaggtgctgt atcgatatgc
     7921 tttggcaatt gcgtgtgccg cagatggaga tgaaatgcca ataaaaaaag cctgagttcg
     7981 atgagggacg aactcaggct agtgttatgt tttacagtaa cacgccgcgg cgcgttggct
     8041 gattagaact ggtagacgat accaactgca aactggtcat cagttgcggc atcggtaatc
     8101 gctgcatcgt ctttatccag caggttaaat ttgtatgctg tgtagacgtt catgttctta
     8161 ttgaagtagt accaggtacc aacttcgata taatttacgc ggtcagcatc aacaccttta
     8221 tgtccattac gagcataaag atctttgcct ttggaatata cgtagcctaa agacggacgt
     8281 aaaccgaagt cgaactgata ctgtacaaca gcttcaaagt tttgggtttt atttgcaaaa
     8341 tgatcattag ccaaaacggt catgttgcgg gtttcaccgt acatcattgc tgcatagata
     8401 ttatttgcat catacttggc tgctaatgac cacacttcag cgttatcacc gtagccgtca
     8461 ccagcttgtt cattagtacg atcagatttg ctatatgcac ctacaaaacc aaaaccttcg
     8521 atgttgtagt ctacagacaa cgcatagcca tcaccgtttt gtttagtaac gccacgattg
     8581 ctgttcttac cttgatattg cacagcaaaa ttcagaccat caaccagacc aaagaaatca
     8641 gagttacggt aggttgcaac accgttggta cgaccgttca tgaagttgtc agcggaagcc
     8701 caggaatcac caccccattc aactaacata tcggtataag ctgcggcatc ataagcaaca
     8761 ccgtagttac gaccatagtc gaaagatcct aactcgttat atttcaaacc tgcgaaggtt
     8821 aaacgggttt tttcctgatt agagccttcg ggcttgctgg cgtcgaggtt gtattcaaac
     8881 tgaccaaaac cagtcagttc gctggtgatt tgggtttcac ctttaacacc gaaacgagca
     8941 taggaggtat cttcgttttc gctgttatta tcatcagtat ttgaccagat gcgttcgcca
     9001 accatcttgc cgtagaaatc aactttattg ccatctttat tgtaaatttc agccgcattt
     9061 gctgcgccag caactaataa cgccgggacc agcattgcca gaacttttct tttcattatg
     9121 tattcccttg tgattataat cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa
     9181 agcacatttg gatactattc tatgaagttc attttatttt aaagattaca tgtaacaaat
     9241 gtatttaaaa gatttcaaaa tgtttctgat ctatttaata taattcagga ttatgaattc
     9301 taaatataaa tattcatgaa aattataaat gtatatcaat atgttatgag ttgagcggaa
     9361 gcataggggg tatcaatgat tgttgcgcca aacggctggt gtgatcacgc gcacattaat
     9421 gaaggcataa aaaaaagccg gcatcatgcc ggcccaaaaa atataaacaa gacgataaaa
     9481 ggctaataac ggaggcatca tgacacagaa aaataaaaag gtataattgt atttagtgct
     9541 aatatattga ttaaatgaat taataaagcc gatctcaaag ataaataaaa tcgctcaaaa
     9601 cgatttttcc tgtattcact gccgttgcgc aaaaattatc tatttgttca aaaaatgagt
     9661 gagtcttgac tggctcatcc aatgtggaaa aatgtgactt ttatcacata atagtgctaa
     9721 gtctgaattt tccgggttat ctcaaaatgg aatacggttc gacaaagatg gaagagagac
     9781 tctctcgcag ccctggtggc aaactggctt tgtgggcttt ctatacatgg tgtggctatt
     9841 tcgtctgggc aatggcgcgt tacatctggg tgatgagccg gatccccgat gccccggtgt
     9901 cgggatttga aagcgattta ggctctaccg cgggaaagtg gttaggggca ctggtcggat
     9961 ttttatttat ggctctggtc ggggcattgt taggcagcat tgcctggtac acccgacctc
    10021 gtcccgcacg tagcagacgc tatgaataaa aactgctaag gtggcgactc tttggcaacg
    10081 gagcgcgtat ggacttacaa cactggcagg cacagttcga aaactggttg aaaaaccatc
    10141 atcagcacca ggacgcggcc catgacgtgt gtcattttcg ccgcgtctgg gctacggcac
    10201 agaagctggc ggcagatgac gatgtcgata tgctggtgat tttaaccgcc tgttattttc
    10261 acgatattgt gagcctggcg aaaaatcatc cgcaaaggca gcgttcttca atcctggcgg
    10321 cagaagagac gcgtcgcctg ctccgtgaag agttcgagca atttccggcg gagaaaatcg
    10381 aggccgtttg tcatgccatt gccgctcaca gtttcagcgc gcaaatcgcc cccttaacaa
    10441 cggaagctaa aatcgtccag gatgcggatc ggctggaagc cctgggggcg attggcctgg
    10501 cgcgcgtgtt tgccgtttca ggggcattgg gcgtggcgct gtttgatggt gaagatccgt
    10561 tcgcacagca tcgcccgctt gatgataaac gctacgcgct ggatcatttc cagactaagt
    10621 tgctgaagct accgcaaacc atgcaaaccg caagaggcaa gcagctggcg cagcacaacg
    10681 cgcatttttt agtcgagttt atggcgaagc tcagtgccga actggcgggg gagaatgaag
    10741 gtgtcgatca caaggtgata gatgcgtttt cacccgcggg ataagcgcgt ggccctaaat
    10801 ggctgtaatt atgttaacct gtcggccatc tcagatggcc ggttaaatct atgcaggaaa
    10861 atatatcagt aaccgattca tacagcaccg ggaatgccgc acaggcaatg ctggagaaac
    10921 tgctgcaaat ttatgatgtt aaaacgctgg tggcgcagct taatggtgtg ggtgagaatc
    10981 actggagcgc gacaatttta aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa
    11041 gtgagaaaga gttcgcccat ctgcaaacgt tgttacccaa accaccggca catcatccgc
    11101 attatgcgtt tcgctttatc gatctatttg ccggaattgg cggcatccgt cgcggttttg
    11161 aatcgattgg cggacagtgc gtgtttacca gcgaatggaa caaacatgcg gtacgcactt
    11221 ataaagccaa tcattattgc gacccggcga cgcatcattt taatgaagat atccgcgaca
    11281 tcaccctcag ccataaagaa ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac
    11341 acattcctga acacgatgtt ttactggccg gtttcccttg tcagccattt tcgctggctg
    11401 gcgtatcgaa aaagaactcg ctcgggcggg cgcacggttt tgcctgcgat accgagggca
    11461 cgctgttttt tgatgtggtg cgcattatcg actcgcgtcg tccggcgatg tttgtgctcg
    11521 aaaacgtcaa aaacctgaaa agccatgacc agggtaaaac gttccgcatc atcatgcaga
    11581 cgctggacga actgggctat gacgtggctg atgcagaaga taacgggcca gacgatccga
    11641 aaatcatcga tggcaaacat tttctgccgc agcaccgtga acgcatcgtg ctggtgggtt
    11701 ttcgtcgtga tctgaatctg aaagccgatt ttactctgcg tgatatcagc gaatgtttcc
    11761 ctgcacagcg agtgacgctg gcgcagctgc tggacccgat ggtcgaggcg aaatatatcc
    11821 tgacgccggt gctgtggaag tacctctatc gttatgcgaa aaaacatcag gcgcgcggta
    11881 acggcttcgg ttatggaatg gtttatccga acaatccgca atgcgtcacg cgtacgctgt
    11941 ctgcgcgtta ttacaaagat ggcgcggaaa ttttaatcga tcgcggctgg gatatggcca
    12001 cgggtgagaa agactttgac gatccgctga atcagcaaca tcgtccacgt cggttaacgc
    12061 ctcgtgaatg cgcgcgctta atgggttttg aagcgccggg agaagcgaaa ttccgcattc
    12121 cggtttcgga cactcaggcc tatcgccagt tcggtaactc ggtggtcgtg ccggtctttg
    12181 ccgcggtggc aaaactgctt gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag
    12241 aggcacaaca tggccgacgt tcacgataag gccactcgca gcaaaaatat gcgcgcgatt
    12301 gccacgcgtg atacggcgat tgagaagcgc ctcgccagtc tgttaaccgg gcagggcctg
    12361 gcgtttcgcg ttcaggacgc cagtctgccc ggacgtccgg attttgtcgt tgatgaatat
    12421 tgttgcgtga tatttaccca cggctgcttc tggcaccatc atcactgcta tctgtttaaa
    12481 gtgcctgcga ctcgaaccga gttctggctg gagaagatag gtaaaaatgt tgagcgcgat
    12541 cgccgcgata tcagtcgctt gcaggaactc ggctggcgcg tattgattgt ctgggagtgc
    12601 gcgttacgtg ggcgcgagaa gctgacggat gaagcgctta gcgagcgtct ggaagagtgg
    12661 atctgcggcg aaggtgccag cgcgcagatc gacacgcagg ggattcattt actcgcttga
    12721 tgcgtcctga atcactggcg caactacggg ttttgccggg aagagatatt ttcccaacgt
    12781 gaccagcacc accgcgaaga caattacgcc gagcgccagc cattcaatct tcgacagcgt
    12841 ttctccgccc actcccgtac ccagcaagac cgcgaccacc gggttaacgt aggcgtagct
    12901 ggtggcgaga gccggactga cattacggat taaatacata taagcgttaa tggcgataat
    12961 cgaaccaaac agcgccagat agccgaccgc aaggaagcct gaaagggaag ggagcgccgt
    13021 cagtttttca cctgcaatca tcgacgcgat cattaacacc acgcctgccg ccagcatctc
    13081 aatcgcaccc gccatcatcc ctacaggtaa ggtaatgcgc gagccataaa ccgagccaaa
    13141 cgcccagcta atcgagccga ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt
    13201 tccaccgcta ttgagcatga tgattccggc aagcccaatg gcgataccca cccattccag
    13261 tttgcgcgtt ttaatgccaa acaggcggct gaagcacagg gtaaagaggg gcacggttgc
    13321 aaccacaacg gcggcgatgc cggaaggaac attttgatgc tcggcaaccg tcaccatgcc
    13381 attaccgaca gccagcaata acaggccaat cagcgcggca ttgagcagcg gacgtagcgg
    13441 ggggagcttg tgtccgcgca gtagcaaaaa tgccatcaat aaaataccgg ctgccaggaa
    13501 tcgaacgcct gccatcatta acggaggcca gctttccacg ccaatccgaa tgacaaaata
    13561 ggttgagccc caaatgatat acaacgcaaa cagcgcgcca aaaagcggta acaactggcg
    13621 gaaacgcata atccctcacg gtggaaataa aaaggtggtt catagtaaac gtgaaaatca
    13681 ttctgctggc gagagatata attgcactta attgttaaaa aaatgttgac ctgtgaagca
    13741 agtatcagag ctgccgtttt tgcttcatac ttacaccctt caacaataaa aatgagaggg
    13801 aatgcttttg gccgggagta gtttactgac gttgctcgat gatatcgcca cactactgga
    13861 cgatatctcc gtgatgggca aactggcggc gaagaaaacc gccggtgtat taggggatga
    13921 cttatcgctc aatgcgcaac aagtttcagg cgtgcgggcc aaccgggaac ttcccgtggt
    13981 ctggggcgtg gcgaaaggat cgctgattaa taaagtgatt ctggtgccgc tggcgctgat
    14041 catcagtgcg tttatcccgt gggcgattac gcctctgttg atgattggtg gcgcgtttct
    14101 ctgctttgaa ggagtagaga aagtgctgca tatgctggag gcgcgtaaac ataaagaaga
    14161 tccggcgcag agccagcagc gtctggagaa gctggcggcg caggatccgc tgaagtttga
    14221 aaaggacaaa ataaaagggg cgattcgtac cgattttata ttgtctgccg aaatcgtcgc
    14281 catcacgctg gggattgtgg ccgaagcgcc gttgcttaat caggtgctgg tgctttcagg
    14341 catcgcgctg gtagtgaccg tgggcgtcta cggtctggta ggggttatcg ttaagattga
    14401 tgacctgggg tattggctgg cggaaaaatc cagcgcgctg atgcaggcat taggtaaagg
    14461 gttattgatc attgcgccct ggttgatgaa ggcgttatcg attgtcggca cgctggcgat
    14521 gttcctcgtc ggcggcggga ttgtggtaca tggtattgcg ccgctgcatc acgccattga
    14581 acatttcgcc gggcagcaaa gtgcagtggt ggcgatgata ttaccgactg ttttaaatct
    14641 gattcttgga tttatcatcg gcggcatcgt ggtgctggga gtgaaagccg tagcgaaaat
    14701 gcgcggtcag gcacattaat actcgcacag gctattatgc aaaattcgcc atcttcaact
    14761 aaggtgaaca agtttcactc gaaaaaagga ggcgagtatg agctttatgg ttagtgagga
    14821 agttacggtt aaagagggcg gcccgcggat gattgtcact ggatactcca gcggtatggt
    14881 tgagtgtcgt tggtatgacg gttacggggt caagcgggaa gcttttcatg aaaccgagct
    14941 tgttccgggg gaggggagtc gttctgcgga agaagtttga aagggtatca ttaacaccag
    15001 gccggatcac atccggcctg gtgtgccaat gcctgcaatg aatgatagtc gcgtcttatc
    15061 atgccaccat tataaaatta tcgctgtttt gcggtacgct atcgccaggt tatgccagaa
    15121 tcataaaaaa gcaggttggg agtcgtcagg gtgcagcacg agacaaaaat ggaaaaccag
    15181 agctggttga aaaaactcgc acgccgcctg gggcctggtc atgtcgttaa tctctgcttt
    15241 atcgtggtat tgcttttttc caccttgctc acctggcgtg aagtagtggt gctggaagat
    15301 gcctatatct ccagccagcg taatcatctg gaaaacgtag ccaacgcgct cgataagcat
    15361 ttgcagtata acgtcgacaa actgatcttt ttgcgtaatg gcatgcgcga agctctcgta
    15421 gcgccactgg atttcacctc tctgcgtaat gctgtaaccg agttcgaaca gcatcgcgac
    15481 gagcacgcct ggcaaattga actcaaccga cggcgcactc tgccagttaa tggtgtgtcg
    15541 gatgcattag tcagcgaggg gaatctcctg tctcgcgaaa atgaaagcct cgacaatgaa
    15601 attaccgctg cactggaagt tggttacttg ctgcgactgg cgcacaactc ctcgtcgatg
    15661 gttgaacagg cgatgtatgt ctcgcgtgcc ggattttacg tttcgacgca gccgaccttg
    15721 tttacgcgca atgttccaac gcgttattac ggctatgtca cccaaccctg gtttatcggc
    15781 cattcgcaac gagaaaatcg tcaccgcgcg gtacgctggt ttacttcgca accggaacac
    15841 gccagcaata ctgaaccgca ggttaccgtc agtgttccgg tagacagtaa taactactgg
    15901 tatggcgtgc tggggatgag tattcctgtg cgtactatgc agcaattttt aagaaacgcc
    15961 atcgataaaa acctcgatgg tgagtatcag ctctatgaca gtaagctgag atttttgacc
    16021 tcttccaatc ctgaccatcc aacagggaat atttttgatc ctcgtgaact ggccttgctg
    16081 gcgcaggcga tggaacatga cacgcggggc ggcattcgta tggacagtcg ctatgttagc
    16141 tgggaacgtc tggaccattt cgacggtgtg ctggtgcgcg tccatacgct aagcgaaggc
    16201 gtgcgcggcg atttcggtag tatcagcatt gcattaaccc tgctgtgggc gctctttacc
    16261 accatgttac tcatctcctg gtatgtgatt cgccggatgg tcagcaacat gtatgttctg
    16321 caaagctcgt tgcagtggca ggcgtggcac gacaccttaa cccgtttata taatcgtggc
    16381 gcactgttcg aaaaagcccg tccgctcgct aaattgtgtc agacgcacca acatcctttt
    16441 tctgtcattc aggtcgatct tgaccatttt aaagcgatta atgaccgctt tggtcatcag
    16501 gcgggcgacc gtgttctttc tcatgctgcc ggattaatta gcagttcctt gcgtgcgcag
    16561 gacgttgccg ggcgggtcgg tggtgaggag ttttgtgtga ttctgccagg cgcgagtctg
    16621 acggaggctg cggaagtcgc agaacgtatt cgcctgaagt taaatgaaaa agagatgttg
    16681 attgctaaga gtacgacgat acgcatcagt gcctcgttgg gggttagtag cagcgaggaa
    16741 accggtgatt atgattttga acaactccag tcactggctg accgtcggct ttatctcgct
    16801 aaacaggccg ggcgtaatcg ggtatgcgcg agcgataacg cttaacgagc ggagaaaaaa
    16861 tggtccagcc cttcacgcca gccttccggt ccttcacgct gcgttcgcca gacgcgggtc
    16921 ggatcctcat catgcagatg caccccttca cggtttagcc ctttcacaat caccgcgtaa
    16981 tccattacct ccagtaaggg cgcatcgttt ggcccatcgc ccaggccaag tgtggttggg
    17041 cgtttgcctg ataattgttg ataggtcgcg ataatccagt tggcggcctg atcttttccg
    17101 gcagaggcat ccaggacgtg ccagaagcgc gcaccctgca taaactgtaa gcccagttcg
    17161 ttcagacgag cggtaaattg tgccatacgc tcgtcactgt cgcgccagat tagcgttacc
    17221 gacgcctcat gcagctgcgt cagcgccgcc tggctacggc ttaatcccgt ccattcggcg
    17281 atggttgcat cgtcgacatc atcaaaagtc gtgaatttaa aatgttcctt ctcgcgtagc
    17341 gtatttaata cctggctgat ttcgccatgg ctaatacctg agatgatgcg tggaaaaccg
    17401 tctatatcct gccattgctc agcaagctgg atcactgcgc cattttcggc aatcagcggt
    17461 aaaccttgta gccccaacgt tttttgcaag tacaacattt ccgctgatgt cttactgcta
    17521 cagagaatga cgggaacatt cgcttcgcgt aaacggctga gccagggggc agctggttgc
    17581 cagtcataac tatgactgtc cagcagggtg ccatcaagat cgctaaaaac cagtagtggt
    17641 tgttgaattg aaaacatggt cgcgcagttc tccccttacc aggatgattc tcaaaacagc
    17701 acaaataatc aaagatgatt ttttcgggag cgatcccgtt gacgccagag ccaccaaggg
    17761 gtgacgcacg tcacatttct attcataagt agcgttaatc attcatatcg cgaatatttt
    17821 cttgtcagcg aaaaaaattg cggataaggt gatgaacaca tcagatttcc tggtgtaacg
    17881 aattttttaa gtgcttcttg cttaagcaag tttcatcccg accccctcag ggtcgggatt
    17941 tttttattgt gcattcaacg attcacttca gagacgctga agtcatgaat atccagttcg
    18001 aaggcatccg ccaattcacg ccatgtatga tattcacgtc catcgacgct aacgtgttca
    18061 gaatcgttct ggctgcgatg aacttcgctt tcgctaattt cattgatcgc cgccagcagg
    18121 gcatcgacat cgacgctgac ctcacgtttt gcggtatcgc tgtactcttt tgcggttttc
    18181 atcatctttc ctcgcaaccg ttactctgtt gttgccgcca gtcgttaagt ccgcctggca
    18241 tctcttaagt atagaccgtc gagaaaatca gcactcgcgc gcgcctggcg ctgccagaaa
    18301 aacgaaattg ttctacactg gcacaaagcc acgggaggaa aacgatgaag gtgaatgatc
    18361 gggtaacagt caaaacggat ggcggtccgc gtcgtcctgg cgtggtactg gcagttgagg
    18421 agtttagtga aggcacaatg tacctggttt cgctggaaga ctacccgctc ggcatctggt
    18481 tctttaatga agcagggcat caggacggta tctttgtgga gaaagcagag taatcttatt
    18541 cagcctgact ggtgggaaac caccagtcag aatgtgttag cgcatgttga caaaaatacc
    18601 attagtcaca ttatccgtca gtcggacgac atggtagata acctgtttat tatgcgtttt
    18661 gatcttacgt ttaatattac ctttatgcga tgaaacggtc ttggctttga tattcatttg
    18721 gtcagagatt tgaatcgttc cctgacctgc catccacatt cgcaacatac tcgattcggt
    18781 tcggctcaat gataacgtcg gcatatttaa aaaagaggtt atcgttgtct cttttttcag
    18841 aatatcgcca aggatatcgt cgagagattc cggtttaatc gatttagaac ttatcaataa
    18901 attttttctg accaatagat attcatcaaa atgaacattg gcaattgcca taaaaacgat
    18961 aaataacgta ttgggatgtt gattaatgat gtgcttgatg tgctgactgt tagaagcatc
    19021 atggatgaaa cagtcctcat taataaacac cactgaaggg cgctgtgaat cacaagctat
    19081 ggcaaggtca tcaacggttt caatgtcgtt gatttctctt tttttaaccc ctctactcaa
    19141 cagatatccg gttaaaccta gtcgggtgta actacataaa tccataataa tcgttgacat
    19201 ggcataccct cactcaatgc gtaacgataa ttccccttac ctgaatattt catcatgact
    19261 aaacggaaca acatgggtca cctaatgcgc cactctcgcg atttttcagg cggacttact
    19321 atcccgtaaa gtgttgtata atttgcctgg aattgtctta aagtaaagta aatgttgcga
    19381 tatgtgagtg agcttaaaac aaatatttcg ccgcaggagt atcctggaag atgttcgtag
    19441 gtattatttt tcggataatc cttaggataa catgataaac gttacggaat tatattaatg
    19501 gcaattcact aataatatca gccagcaaat taaacatctc actgaataaa tgttcgcaaa
    19561 aaggcgcaat taacggcatt aatgccgcca ttaaagagat accgacagtc agagttaatg
    19621 gaaatccaat aacaaaaatg gataattgcg gggccatacg attcagtaaa cctaatgcca
    19681 gattcagggt cagcagcaga gtaatgagcg gtaacgccag catcagcccg ttaaggaaaa
    19741 tcaaactccc tgctttggtg agtgccagaa acgcattgct gttcaacggt tcgccaccaa
    19801 tcggcagggt gtgaaaggta tcgaccagca gtgaaatcaa ccataaatga ccgttaaatg
    19861 tcaggaacag cagtaacgcc agcatatcca tgatacgcgc tagaacgggc atgttgagat
    19921 ggctggccgg atcgacaaac gtcgcaaatg acagccccat ttgcagaccg ataatttcgc
    19981 cagcggttcg cacagcggca aaggcaaatt gcatggtgaa accaagcgca atgccgatca
    20041 ggatctgctg cacggccagc cacagagcaa agaacgaaaa aacaggaaca tcgttggcag
    20101 gtaatgatgg ggcaatagcg aacgtgatca tcattgccag acccagtttt acccgtttcg
    20161 gcacgctgcg ttcgctcaga attggcgcgg tagaaatgag cgccagcacg cgcagtaacg
    20221 gccagaagta cagacttagc caggaaagcc attgatcgct tgtcacctgc atcattgtac
    20281 gggctacccg atgatatacg gcaggttagt gaacaaggtg cggacgtaat ccagcaacag
    20341 attgagcatc cacggtccgg caataataat ggcaataaat acggcgatga tttttggaat
    20401 aaacgacagc gtcatttcgt tgatctgcgt ggcggcctgc aaaatactga tgataaggcc
    20461 cgtgaccagc gccaccagca acagcggggc agccagtgcc agcgcgactt tcatcgcttc
    20521 agtccccatc atcatgaccg attcaggtgt cattttgcct ctctagctgt aaaagctctg
    20581 cgccagcgaa ccgaccagca attgccagcc atccaccagt acaaacagca tcagtttaaa
    20641 gggcagagca atggtggcgg gggggaccat catcatcccc aatgccatca acacgctggc
    20701 aatcaccagg tcgataatca aaaaagggat gaaaatcgtg aagcctatct gaaatgcggt
    20761 tttcaactcg ctggtcacgt aggccgggag caaaatgcgc atcggcacgg cttcaggtcc
    20821 ctgcaacggg ccagtattcg ccagtctggc aaacaaccct aaatccgcct cacgggtctg
    20881 acgcagcata aactcacgca gcggctgcgc ccctttttcc tgcgcctcct gcattgatat
    20941 tttctcttcg ctgaatggct ggtacgcatc gacataaatt ttgtcgatca ccggcgacat
    21001 aataaaaaag gtcaaaaaca gcgccaaccc cagcaatacc tggttaggtg gcgcggaggg
    21061 tgttcccagc gcgttacgca ataaaccaaa aacaataatg atgcgggtga aactggtcat
    21121 catcagtaaa attgccggaa taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg
    21181 gagcgaccag ctttgtccac cgccaggcag cggctggctg gtgatacccg gcagttgcgc
    21241 gaaggcgagg ggcgtaatca gccagagaag gacaggggcg acagacaaca aacgacgcat
    21301 caggatctcc cgctacgctt aagcaagttt ttcatgaccg actgaaaatc gggctgcggt
    21361 atctcttccg ttggtgctga agggggaagt ttatgcagca gattgatttg ccctgcggta
    21421 acgccgagca ccagccgtgc atcttccaca tcgaccacca caacccgttc acgcgcgccc
    21481 agcgaagcac tggcgctaat tttcagaccg ttaacgccag tgcgtttagg gacaaatccc
    21541 aaccgtttta ccagccaggc agcagcgagg atcagggcaa taatggcgat gagtgcgccg
    21601 ctcacctgca gcagtggcgc agcagaaacc ggcgcggaag attgcacagt agcgtggtta
    21661 ttcatcacta acggctcagg cggcgcattc gctcagacgg agtaatgata tcggtgatcc
    21721 gcacgccata tttatcggca acgaccacca cttcgccctg ggcaattaaa taaccgttga
    21781 tcagaatatc cagtggttcg cccgccagac cgtccagcgc cacgacggac ccttgcgtca
    21841 gacgcaacag ctctttgatg gtcattcgtg tgcggcccag ctcgacggtc agcttgaccg
    21901 gaatatccat tatcaggtcg atatcctgca acgttccgct gacatcacca ccgccaaatt
    21961 gctggaacac cgcgtcggca gcgcttttgc tgctggttga tttttgttcg ctcaacgctt
    22021 cagcccacag atcgtccatt gcgccgttgt tgtcatcggc cggattattc atgtcactca
    22081 tttgggctgt tcctcgttca gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa
    22141 cgcatattga ccgttgagag tgccgtactg actggtcagc accgggacgc cgtcaacatg
    22201 ggcgatgatg cgatcgggtt tttctatcgg caggacgtcg ccgggtttca gttttaaaat
    22261 ctgcgacagg cgtagcgaga tatcggcaaa gttggcgacc agctccagct gtgaatgctg
    22321 cacctggcgc accaggttat cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg
    22381 cgggttaacc aacaattccc gtagcggctc gatcatgctg aatggcaggc agatattaaa
    22441 ttcgccggtc aggttgccaa tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg
    22501 cgaggtggtg atattggtaa atttcacctg catttccgaa cgcacgtact caacttccag
    22561 cggattaatc gccttccatg cgtcgctata gccttcaagc gccagtttca acatgcggtt
    22621 gatgacgcgc tgttcggtat gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc
    22681 atcgccgcca aacaggttat ccacggcgat aaacaccaga ctcggtgaga acaccaccag
    22741 cccagtgccg cgcagcggtt tcagatggat aaggtttagg ttggtcggca ccggcaggtt
    22801 gcgggcaaat tcatggtacg gctgaatgcg gatggccccg acggttatat ccgggctacg
    22861 acgcagcagg ttgaacagcc catacgaaaa tggcgggcaa agcgctcatt aatgatttcc
    22921 agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta cggacgaatg
    22981 tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc accattcaac
    23041 agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc gcagaataaa
    23101 agcggtatac agcacgtcgg tgacatcctg tttcggttgc ccggcaacaa gcggggtgga
    23161 aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg ccagtacggc
    23221 agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca aatactcact
    23281 cagccgcgag cgggtagctt catctttcag gcgcagggtt acgccgatat aaagcacgcg
    23341 atccgcatcg cccaaattga ccgtgaaggt atccagcgcg tagaagaccg gggagggtac
    23401 gacgcgttgc tgcgctttgt cgtcagcggc aacctgatgc gaatgccagt agctgtaacc
    23461 tgcgctggca caggccgcga gggtaatgaa taccagaatc gggatccaaa gcgatcgctt
    23521 gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt cgctactgct
    23581 tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg cggattacgg
    23641 tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac gcgcccttgt
    23701 atagagacgg gaaccggaag cgtatcgtcg tcttcccccg ccagaggttc atggtttact
    23761 gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa gctttcgcca
    23821 ctgatgttgc tttgccctaa ctgaatgcca ctttcggcca gctgcgtacg cagtaccgga
    23881 agcgctgctt ccagcgcggc gcgtacatgc tgatgcggtg aaaccatctg gatttgtgcc
    23941 tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg gtgcagacgc
    24001 aactctgcac tttgttgccc ctggcgggtg aatagcgaaa tatgctggct taatgattgt
    24061 tgccattcgt gagaacccag cggtgcactc aaaacaggcg cggcgacggt gggcagtggc
    24121 tgtgtctggt gtggagtaat tagcgggctg gcggcagcgg tcaccggtga cggtgtgctg
    24181 atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg agctggcgtg
    24241 ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt tgtgaagagc
    24301 gttggtttct ctgccggtaa cacggttgac ggcacatcag tcactttggg cgtattgtca
    24361 aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgacgtcttc attcagatca
    24421 tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt catcgtctga
    24481 gcggtggtta acggtgttga tgtggattgt tcgacgttga tgacaggcag tgtttcatcc
    24541 acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat cagcagctcg
    24601 ccttttgtcg tgggcttatc tgttgccacc agcaactggg gggccgcttt gtcggtagtt
    24661 gtctcgcctg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc atcgctggct
    24721 ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa ttaagggcgc taagcgaatc
    24781 attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc tgatcgaggc
    24841 ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc caggcctgca
    24901 aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc gtccactgat
    24961 taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac tgctgatagt
    25021 tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta ttgcgatatt
    25081 cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac ccgcgacgca
    25141 tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct ttcagggtcg
    25201 ccagcgcgcc atgttctgcc atctgccgtt atctcctggg ttatgacact gtcgggaaaa
    25261 tacgctccag cccctggaga gacgcttccc agtccgcgcg ttcaaaaatg ccttgttgca
    25321 aatagccctc cagctgcggc cacagggcga tggctttgtc gagcatcgga tcgctgcctt
    25381 tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac aacagctgtt
    25441 tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt gcgcggctga
    25501 tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg cgagacagca
    25561 caatgtgacc gtcgaggatt gcccgcgcag agtcggcaat ggggtcctgc tggtcatcgc
    25621 cttcagtgag cacggtataa aatgcagtaa tcgatccgcc gccgctaatg ccatttccgg
    25681 cacgctcgac cagtgccggt aatttggcaa acaccgacgg gggataacct ttagtggcgg
    25741 gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg gtgagggagt
    25801 ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg cgcgtggcat
    25861 aggcggcacc ctgcattcgc aggagaggag aaacatccgc cggagcggca atcaccacgg
    25921 agcgtgcacg cccttcggca ccgaggatat tctcaataaa atcttttact tcgcgtccgc
    25981 gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc atcatcccca
    26041 gcagcacact tttaccaacg ccggacccgg caaacagtcc catacgctgc ccacgcccaa
    26101 cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca atcggtgtac
    26161 gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta tcgggggagg
    26221 gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac gccggaccga
    26281 gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca taaacccgcg
    26341 cgccgggcag gacaccttcg acttcctcca gcggcattaa aaacagccgt tgaccgttaa
    26401 agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt tcaatgacac
    26461 aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg gtagcgcggg
    26521 ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca aagttatcca
    26581 gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg ctgccagacg
    26641 gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat cggcggagac
    26701 tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat gcaaacttaa
    26761 ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc gcagctgtgg
    26821 tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga tcagtgccga
    26881 gttatccact gttggcgtct gaccgatgac ctgacgtgcc gcctccagcg ccatctgcat
    26941 caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt cgctgagcag
    27001 ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca gaccttgctc
    27061 cagcccccgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc gaccttcggc
    27121 aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca gttgctgctc
    27181 aaggctgggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg gcacaaactc
    27241 tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat tatcagacat
    27301 aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg cgcacaatca
    27361 gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca cggttggcga
    27421 gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat ttctcgcgca
    27481 gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact tcctgcaaca
    27541 gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac atctcgtcga
    27601 taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg gcttcttcct
    27661 gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc attttgctgc
    27721 gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc gccagcgcgg
    27781 ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc agacgttcat
    27841 cgaacaacgc cagaatatcg gcggcctggg cgcgcttcag atgcaccaga atggtggcga
    27901 taatttgcgg atgttcatcg cgaatcagat cggcggcact ctgcggctcc ataaagttga
    27961 gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc aggctggcgg
    28021 cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg gcgttgatat
    28081 tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatcg gttagctgct
    28141 tgttggagat ctgcgtgacg ttcgccattg cagcgcttag ggtttgcact tcgcgctggg
    28201 agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc aggatgacgc
    28261 ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg aatgaccagc
    28321 gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat gacttctgcc
    28381 cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag gcggacttcc
    28441 accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt catcgcctcg
    28501 gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc caccagcagt
    28561 accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg ttgccagaac
    28621 ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac attgagcgag
    28681 tcaccgcgtt tttcagaaaa gcccatcgcc tcgcgggtca gatcttcaat ttgcttcatc
    28741 tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt caccactacc
    28801 gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat ggtgcgatcg
    28861 acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt gctggtggtg
    28921 ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg cgcgttattc
    28981 gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc ggaaccgctt
    29041 tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc accgttaggg
    29101 cgatactgtt cttccgtttg ttctttactg gcgaagtcca gctgcgccgt gacctgggcg
    29161 tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg ctgaatacgg
    29221 ccttcgacat cgctggcata tttcaactga gcatcattaa gatcgcgccc gctggtattg
    29281 gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg cggcagacca
    29341 gcaacggcgc tggaaaccag atgcacaatg gcgctaattt gcccttcatc gagtgcgcgg
    29401 ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg gacgaataaa
    29461 gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat agttgcgatg
    29521 gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct gaactggctg
    29581 ataccaaact tttcctgatc gagtagttcg aaaccgaccg cgccgccttt gggcaaacct
    29641 tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc aatagcgccg
    29701 ctggcttcgc tgaagcggta aggaatattc atttgcgtca gttggctgac aattgcgcca
    29761 ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt cgcccacagg
    29821 atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg aattttcgga
    29881 ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt cgcattcatc
    29941 gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag actcactccc
    30001 gggtcagcaa actcgaaaaa ttgacgctcc cattatttgt gctttcgttt ttttctatcc
    30061 gccaataaac ccgttttttt gttgctattt agcgcctttg tcttattgac ttactggtag
    30121 gctttgctac cagaaattat ccaggagatg aaaatgtcag cgatacaggg gattgaaggg
    30181 gttatcagcc agttacaggc tacggcgatg agtgcgcgcg cgcaggattc actgccgcaa
    30241 ccgaccatta gttttgccgg gcagctgcac gccgcgctcg atcgcattag cgatacacaa
    30301 acagctgccc gcacgcaggc agaaaaattc accctcggtg aacccggcgt ggcgttaaac
    30361 gatgtgatga ccgatatgca aaaagcctca gtttctatgc aaatggggat tcaggtgcgt
    30421 aataagctgg tggcggcgta tcaggaagtg atgtccatgc aagtgtaggt tttgtaacct
    30481 gttgttaatt acatcccatc tgcccgcgtg gggcatggat ggggcaaact caccctattt
    30541 ctggtttaaa atggatacct gatcctgaaa gttttctgcc atccacgatc cgtatacgca
    30601 ataaaccctc tgatcatcag aggcctcatt tgatacacat caccacgtcg gatgttgtta
    30661 cgaccgcaga cgcaacagta acgtttgaat aatttaatct ttgttgatgt ttttattcat
    30721 attccgggct agggaaataa tcaggcgtaa ttgtgtcact ggaatagagg tttcttgtgg
    30781 cgcatgtgga aggagcgtct actttgtcta atagagattt cttatgacgc agtattattg
    30841 atcaatagtc tcctggctta tcaaaccggt tgaattaact ccgcgccctg gtttttgaca
    30901 ttacctaccg cgcgcgatac cgggtgccag gtgaactggt ttgctggcac acagccgttg
    30961 gtagcaattt ctgatgcttc tttaccgcca atctcttgcc gcatccattc ccgcgcagct
    31021 tctggcgaca gtaccagcgg gcggcggtca tgaatatcta ccagaccctg gtctgctgct
    31081 gcagtgacaa ttaaaaatcc ttcggcttcg tcaccgcgct caaacggtgt gctacctatc
    31141 gcggccataa aaacaggttg tccgtcagca cgatagataa aataaggctg ttttttgtcg
    31201 ccttcttttt tccactcaaa ccagccatcg gcaaaacaga ttgcccgacc atgttgccag
    31261 agcggtttaa acatacgact ggtggccgca gtttctacgc gggcgttaat cagcggcggt
    31321 ttatcccacc atccgggagc atatccccag aaaaccggat ccagatgaag gtgttcatca
    31381 cgttcactga gcagaagaac tttggttccc ggcgcgacgt tgtatctgcc aatgggttcg
    31441 ggatcatagg gaatatcgcg ttcaatatct tccgcgagaa gcgcaaggta atcttcacgc
    31501 gtttgggatt gggcaaagcg tccacacata gcaacctcct gagagcacac tgaaaagaat
    31561 aggagagggg gcgggtaaaa tctatgtggt acagaggcaa cgatattccc gctgttggcc
    31621 acagcgggaa tgagggatta cttctgaatt aaataacgaa tggtcggccc gtcttgctga
    31681 atatccagca ccgtatagcc gtgattacga gcatccagtg gaatattatt gatcgactgc
    31741 ggacagtcgc tcaccacttc caggatctca ccttttttta actgcggcat cgcctcaagg
    31801 gttgcgactg ccggataagg gcagggttca cccaccatat caagacggta atcaggaatg
    31861 atatttttca tgcgatctcc ttagcagtct gcggtgccgc acggcggaag aagcgttttt
    31921 cccagccgat aatcaacatc agcgcagtaa acaacagcaa atatgtcacc agcaggccac
    31981 ccatcgggcc aaaggttttc agcaggttga ttttgtccca gtcggtggcc agcgccggag
    32041 cgaaatcatc ccagtaatac gccagaatgg ttgagccgat cacattgccc agaccgaccc
    32101 accagtagtg cacctgacct tctaccgcgc ggtacatcca accggtttcg cagccgcctg
    32161 ccagcacgat gccaaaacca aacagtaaac caccaattac cgcgtttggt cccgcccaca
    32221 tgattttggg ttcaacgcct aactgtacgt aactgaagat cccgatggca ctcaccgcca
    32281 taccgataat gattgctttc gccatatggg tacgtccggt gatccacata tcgcggaacg
    32341 ccgaagtaaa gcagatttgc gcacgttcaa tcagtaaacc aaagccgacg ccaaacagca
    32401 ttgccagccc cagttttggt tggttcatcg ccgtgagcag cgcccagccc agcataccga
    32461 aaaagaccag catcccgaga cgaaaacgac gccgcgcctg atccggtttt tgcgtcagcg
    32521 gtgaggcggc agaaactttc tgcattttca cgggaatacg gaagatgggc agcagggtaa
    32581 agcgtgcgcc aaaccatgaa ccaatggcag tggcgatggc aaagaaccag gcatgcagcg
    32641 agaactgagg aataccggta aagaacgccg ccaggttaca gcccattgcc agacgcgcgc
    32701 caaaaccagc gataataccg ccaataatgg cctgcataat gcggatacgg ctgcgtggca
    32761 tgcgcagttt gacattgttg gcccacagcg ctgcggcaaa gcagccgcca aacatgccga
    32821 ggatcatcat cccgtcgatg cgggttaatg gcgatccttc cagatggata attttaaaat
    32881 aaccccactc ttcagcatgg acgccaaaca gctgcaggag ctgaccgccc caacgggtaa
    32941 attcacccgt gacagcccaa aaggtgccag taatgccaaa atagtaagtg gagagaatac
    33001 ccgccgcgat gaccgcaggg atgggtgccc agaatttaat caaccaggcg tgtttgaatt
    33061 gctgccatga catgaatgta gcctctgaac tcagaaggtt tgaaacaaga atcgcgaatc
    33121 atacatcatg caaatttatt ttcggggtaa taattcacaa aaataatagt tagatcaaat
    33181 ttctctctga acctggaaag gctggcgaca tcagggcttt aatgccacaa tcctcttttc
    33241 ttctcatgca ggacgaatga tgaaaaaact cgcaattgca ggcgcgctca tgttgctggc
    33301 gggctgtgcc gaagtcgaaa actataacaa tgtcgtgaaa acccccgcgc cggactggct
    33361 ggcgggctac tggcagacca aagggccgca acgtgcgctg gtcagtccgg aagcgatagg
    33421 cagcctgatt gtcactaaag agggcgatac cctggattgc cgtcagtggc agcgggtgat
    33481 tgccgtgccg ggcaagttga cattgatgtc tgatgatctc actaacgtta ccgtaaaacg
    33541 tgagctgtac gaggttgagc gtgacggtaa caccattgag tacgatggta tgacgatgga
    33601 gcgtgtggat cgcccgacgg cagagtgtgc cgccgcgctg gataaagcgc cgttaccgac
    33661 gccactgccg taatgaaatc tccccggctt gccgccgggg atgaatttaa atcacctctt
    33721 cgataaccca cacgctgacg ctgccgccgt tgcaaaagaa ggtcgcttca ccgttttcgt
    33781 cggttactac gctctcttgc cggttcccca gaaaatcacg ccaggtttta ttgccgtaat
    33841 tctctcccag attaatggtt ttttcgccat catccccgtt cgacatgacc accacgcagc
    33901 cgggatattc gtcagtgcca ctgcggctaa aggcaatgca gttcggatgg tcgaaaaata
    33961 acgtctgtac accgtgggcg aaacgctgac gggcgagaat taactcatca agctgttcga
    34021 ttattggcat atctatcgga taggtttgcc cgtcaccgcc gacatcttcg taatgcgcac
    34081 cgtagaggtc cggatagaat accgaaggaa cgccattttc ccgcaacaaa attaaggcat
    34141 atgccagcgg tttaaaccac ggttcgaccg gcgcttcgag ggcttgcaac ggttgggtgt
    34201 cgtgattggc aacgagcgtc acggcgtgga aaggatcggc ttccactaat gtacccgtga
    34261 aaatctgcgt catgtcgtag tcacgcccca tgcgcgatgc ttcatggaat ttcatctgca
    34321 gcggcgcatc gaacagcatg gttttgcctt ccacctgatc aatatacgtt tgcagtttat
    34381 cgacttcatg cgaccagtac tccgccacaa taaacagcgg ctttggcgca acttcctgta
    34441 cgtgttcgat ccactcttta taaaaccacg ccggaatatg tttgaccgca tcaagacgaa
    34501 aaccgtcgca ttgcgtttgt tccatcaccc agcgcgccca gtatttaatc tcttccgtca
    34561 cggcatgatt gcgaaaatcg atattctcgc ccatcagata atcgaaatta cctaattcat
    34621 catcaacctg atcgttccag ccttcgccgg tgtagtcgtt aacaatttta aatatgccat
    34681 cttcatcagg gttttcgata tggtcgatac cgctaaaaca tttgaaatcc cagataaact
    34741 tcgagtattg cccggcacgc gcggggaagg tgtaacgcgt ccagccttca cactcaatga
    34801 tttcttcgtc aatttgcgta cggtcatcag catttacacg ctgcacgcga atagcttctt
    34861 tttcatccgc gcccattttg tggttgacta ccacgtccaa cagcaccgca atgtcattac
    34921 gtttcagggc gtcaatggcg gccagcagtt gtgctttatc gccatattta gtagggatgc
    34981 tgcctttctg atcaaactcg cctaaatcaa ataaatcata ggagtcgtag ccgaccgaat
    35041 acccgcccga tgcgccttta taggcgggcg gcaaccagac catattgata ccaatatcat
    35101 taaaaccgtc ggcgcgctcg gccagttcag gccagagctt accgccttcc gggtaatacc
    35161 agtgaaaaca ttgtaacagc gtgggattac gcattcttcg actccattca aggggaacat
    35221 tagaagcgta gccgtaatcg gattattcgc gaaccattag ctcgttaaga ttcacgagaa
    35281 gttatcttgc ggagccagca caaagccgcc ctgatcgcca taggcactta acaccgattt
    35341 ttgcaccgat gactgaccga ccagtttcgc cagttcatcc atacgaatct gtaataactg
    35401 ctttaccttg ctttcgttgt cgagaatcag gcgcagcatc gggcggagct gctcctgcat
    35461 cgtggtggac ggggcaacct cttcagtcag gtgtgctatc tcctgcaccg cattcacata
    35521 cgccatttcg ctggcgatga gttcgtccca ttgttcttcc gttgccaggc gtaacatgag
    35581 ctggcttttt tcgacgagtt gttgccaggc gaaatacaaa tgcggtgcat ggttcatcag
    35641 actgggtcct gaatcaaaga aggggagagt aacgactctt tccaggcatc ggcaatatta
    35701 cgcattaatg cttccacttc ttcgactgcg gagacatcgt tgcgtaaatt ggcttgcagc
    35761 aagcgcctga ccatatagct ataaagagca atcaagtttt gggttaattc gtctttgctc
    35821 tcttcatcaa gactcacccg aagtccgttc tcaatgatgt tgatcgcttt tgacaaagag
    35881 acgcctttgc cttgctgatt gttgtcctgc ataaacaggc gcgctctaac cagtgcgctc
    35941 agcactccat caaatagcat ggtgaccagc tgctgctggc tggcgctcat tacggcgctt
    36001 tcgacgccaa tttgtgcata ggcttttgag ccgctcgcgg tatacatgct gacctccgtg
    36061 atcaggaatt actcattgca gtaaattgtt ggctgagata ggtactggtg ttattcagtt
    36121 tgctcatcat ggtatctaat tgcgtaaact gtgcagtgta acgcgccacg gtgtcatcaa
    36181 tgctggcact gacggataga tactgtttgg tcagtttttt cagtgtggcg ttaatactgt
    36241 cctgtgcgct atcaataatg ccatcatcgg caaggtaacc ttttacctcg gtggcaattt
    36301 tggtggtaat tcctgtttct ttaccatcgc ctaccagcaa ttcacgtacg gatgctgtat
    36361 tttcattcag gacttttttg agtttatcat catcaatttt cagcttaccc gtcgtgccgt
    36421 cctgcttaat cccgatctcg tttaatgttt tgaaggcacc gtcgctggca ccgtttgcaa
    36481 attgtgcgcg gatgccgctt tgaattgtac gtacaacgct gtctccgaga agtgcaccat
    36541 tatttttatc ttgtgcttca gcacctgggt ctacttcttt atattttgtc agggtgttaa
    36601 aggtgtcaat gagggagtta tatgaatcca cccagccttt aattgcttcg gttgctttat
    36661 cgttgctttt tgtcaccgtg acccggacgt cagtgacttt tttggtcaaa tcgagagtca
    36721 ctccctgagg agcatcggta attttgttac ttgaacgttc aatatcgata ccgttaacgt
    36781 ttagctgtgc attttgcgcg ttaaccagtt ccttcatatt cccggtgttg gtcttgctgt
    36841 cataagccag cagatcattt aatttactgt caccctcaac agatatggtc attttattgg
    36901 ccagaccttc actggcggtc aataccaatt gatagttacc atctttgact ttaacgatac
    36961 tagcggaaat tccactatcg gcatcgttaa tggctttgct gatctcatcc aatgacgtct
    37021 gatctttatt gagcttgatt tccagcggct ctttacgacc gtcttgggta atttttattg
    37081 tgcgtgtatc tgatgactca tcacccagtg catcttttgt tgatgcaatg attgtcgggg
    37141 aatcagttcg taaagattgc gcctgcgcca gttgggttac gctgatggta tagataccgg
    37201 gagcagcacc tgcttcggtg gaaactttaa ggtcttcatt gctactggta acggtagtgc
    37261 ttttgaacag atcggcttta tttaacgcag tatttgcagt ctggaatttt tctaatgcac
    37321 tttttaatgt gccataggct gtcagacgtg cggtattgtc actctgttgc tgagtaattg
    37381 ggtttaagcg ttttttttca gcgatggtca ggttgtttag caatgtatcc agcggcagat
    37441 tcgaaccaac gccaagagat gtaaatgaag ccatacctaa ttccttttga ttgcaaacag
    37501 tagataagcg ggttatcggc aatttgtgcg caaagtttaa tagttgattc acgaagatga
    37561 tgcgcagagt agagttgtat attacggata tttcagccgt taagaaaaaa caggagagaa
    37621 actaaaattt tctaaagttc gaaattcagg tgccgataca aggggtacgg tgagaaaccg
    37681 tgggcaacag cccaataaca tcaagttgta attgataagg aaaagatcat ggcacaagtc
    37741 attaatacaa acagcctgtc gctgttgacc cagaataacc tgaacaaatc tcagtcttct
    37801 ctgagctccg ccattgaacg tctctcttct ggcctgcgta ttaacagtgc taaagatgac
    37861 gcagcaggtc aggcgattgc taaccgtttt acagcaaata ttaaaggtct gactcaggct
    37921 tcccgtaacg cgaatgatgg tatttctgtt gcgcagacca ctgaaggtgc gctgaatgaa
    37981 attaacaaca acctgcagcg tattcgtgaa ctttctgttc aggcaactaa cggtactaac
    38041 tctgacagtg acctgacctc catccagtcc gaaatccagc agcgtctgag tgaaattgac
    38101 cgtgtttctg gtcagactca gtttaacggc gttaaagtgc tggcttctga tcaggatatg
    38161 actattcagg ttggtgcaaa cgacggcgaa acaattacta ttaaactgca ggaaattaat
    38221 tccgacacac tgggattatc tggttttggt attaaagatc ctactaaatt aaaagccgca
    38281 acggctgaaa caacctattt tggatcgaca gttaagcttg ctgacgctaa tacacttgat
    38341 gcagatatta cagctacagt taaaggcact acgactccgg gccaacgtga cggtaatatt
    38401 atgtctgatg ctaacggtaa gttgtacgtt aaagttgccg gttcagataa acccgctgaa
    38461 aatggttatt atgaagttac tgtggaggat gatccgacat ctcctgatgc aggtaagctg
    38521 aagctggggg ctctagcggg tacccagcct caagctggta atttaaagga agtcacaacg
    38581 gtgaaaggga agggggctat tgatgttcag ttgggtactg ataccgcaac cgcttctatc
    38641 acaggtgcaa aactctttaa gttagaagac gccaatggca aagatactgg ttcatttgcg
    38701 ttgattggtg atgacggtaa acagtatgca gcgaatgttg atcagaaaac aggagcagtt
    38761 tccgttaaaa caatgtctta cactgatgct gacggtgtca aacacgacaa tgttaaagtt
    38821 gaactgggtg gaagcgatgg caaaaccgaa gttgtaactg caaccgatgg caaaacttac
    38881 agtgttagtg atttacaagg taagagcctg aaaactgatt ctattgcagc aatttctacg
    38941 cagaaaacag aagatccttt ggctgctatc gataaagcac tgtctcaggt tgactcgttg
    39001 cgttctaacc taggtgcaat tcaaaatcgt ttcgactctg ccatcaccaa ccttggcaac
    39061 accgtaaaca acctgtcttc tgcccgtagc cgtatcgaag atgctgacta cgcgaccgaa
    39121 gtgtctaaca tgtctcgtgc gcagatcctg caacaagcag gtacttctgt tctggcacag
    39181 gctaaccaga ccacgcagaa cgtactgtct ctgctgcgtt aatttacact tatcgctcaa
    39241 accccgccac cggcggggtt ttttctgcat ggaatttacc ggtacccccc aaataacccc
    39301 tcatttcacc cactaatcgt ccgattaaaa accctgcaga aacggataat catgccgata
    39361 actcatataa cgcagggctg tttatcgtga attcactcta taccgctgaa ggtgtaatgg
    39421 ataaacactc gctgtggcag cgttatgtcc cgctggtgcg tcacgaagca ttgcgcctgc
    39481 aggttcgact gcccgcgagc gtggaacttg acgatctgct acaggcgggc ggcattgggt
    39541 tacttaatgc cgtcgaacgc tatgacgccc tacaaggaac ggcatttaca acttacgcag
    39601 tgcagcgtat ccgtggcgct atgctggatg aacttcgcag ccgtgactgg gtgccgcgca
    39661 gcgtgcgacg caacgcgcgt gaagtggcac aggcaatagg gcaactggag caggaacttg
    39721 gccgcaacgc cacggaaact gaggtagcgg aacgtttagg gatcgatatt gccgattatc
    39781 gccaaatgtt gctcgacacc aataacagcc agctcttctc ctacgatgag tggcgcgaag
    39841 agcacggcga tagcatcgaa ctggttactg atgatcatca gcgagaaaac ccgctacaac
    39901 aactgctgga cagtaatctg cgccagcggg tgatggaagc catcgaaacg ttgccggagc
    39961 gcgaaaaact ggtattaacc ctctattacc aggaagagct gaatctcaaa gagattggcg
    40021 cggtgctgga ggtcggggaa tcgcgggtca gtcagttaca cagccaggcc attaaacggt
    40081 tacgcactaa actgggtaag ttataacgtc agtaaatgcc gcactttaat tttgactacc
    40141 aggagttctt aatgatggtg cagcacctga aaagacggcc attaagccgc tatcttaaag
    40201 actttaaaca cagccagacc cattgcgcgc attgccgtaa attactcgac cgcattaccc
    40261 tcgttcgcga cggcaaaata gtgaataaaa tcgagatttc ccgcctggac atgctgcttg
    40321 atgaaaatgg ctggcaagtg gaacaaaaat catgggcggc attgtgccga ttctgcggtg
    40381 atttacattg caaaacgcag agtgattttt tcgatattat cggctttaag caatttcttt
    40441 ttgagcaaac tgaaatgagc cctggtacgg cgcgtgaata tgtcgttcgt ctgcgccgtt
    40501 tggggaatca tctacacgag caaaatattt ccctcgatcc gctgcaggac ggtttccttg
    40561 atgaaatcct cgccccgtgg ctgcccacca ccagcaccaa caattaccgc atcgcattac
    40621 ggaagtatca acactatcag cgtcaaacgt gtaccggact tgtgcagaaa tcctccagcc
    40681 tgcctgcttc tgatatatat taaataagaa taagatgtag cggagttgtt tttgtgttta
    40741 caaacaatgg ctctacactg caaacagaca taacaacatt cggggtaaat atgaaattag
    40801 cacatctggg acgtcaggca ttgatgggtg tgatggccgt agcgctggtt gcgggcatga
    40861 gcgttaaaag ttttgcagat gaaggtctgc ttaataaagt taaagagcgc ggcacgctgc
    40921 tggtagggct ggaaggcact tatccgccgt tcagttttca gggagatgac ggcaaattaa
    40981 ccggttttga agtggaattt gcccaacagc tggcaaaaca tcttggcgtt gaggcgtcac
    41041 taaaaccgac caaatgggac ggtatgctgg cgtcgctgga ctctaaacgc attgatgtgg
    41101 tgattaatca ggtcaccatt tctgacgagc gcaagaaaaa atacgatttc tctacccctt
    41161 acaccatttc cggtattcag gcgctggtga aaaaaggtaa cgaaggcacc attaaaacgg
    41221 ctgctgatct gaaaggcaaa aaagtgggtg tcggtctggg caccaactat gaagagtggc
    41281 tgcggcagaa tgttcagggc gtcgatgtgc gtacctatga tgatgacccg accaaatatc
    41341 aggatctgcg cgtaggccgt atcgatgcga tcctcgttga tcgtctggcg gcgctggatc
    41401 tggtgaagaa aaccaacgat acgctggcag taaccggtga agcattctcc cgtcaggagt
    41461 ctggcgtggc gctgcgtaaa ggaaatgaag acctgctgaa agcggtgaat gatgcaattg
    41521 cggaaatgca aaaagacggc actctgcaag ctctttccga aaaatggttt ggtgctgatg
    41581 tgaccaaata atcagcataa tgacaaaaaa gggcgctttc actagcgcct tttttattta
    41641 cgcgttttca gcgtgcataa taagaaaatc acaacaacac acatcggagg ctgttatgcc
    41701 actgcataat ttaacccgtt ttccacggtt ggagtttatc ggcgcgccaa cgccgctcga
    41761 atatctgccg cgcttttctg attatcttgg acgggaaatt ttcatcaaac gggatgacgt
    41821 cacacccatg gcaatgggcg gcaataaatt acgtaagctg gaatttctcg cggcagatgc
    41881 cctgcgcgaa ggtgccgata cgctgattac tgccggcgcg atccagtcta accatgtgcg
    41941 ccagactgcc gcagtcgcgg cgaaactcgg tctgcactgc gtggcgctgc tggaaaatcc
    42001 tattggcaca accgcagaaa actatttaac caacggcaat cgtttgttgc tggatctgtt
    42061 caatacccag attgaaatgt gcgacgcact gaccgatccc aatgcccaac tggaagagct
    42121 ggcgacgcga gtcgaagcac aaggctttcg cccgtatgtc attccggttg gcggttctaa
    42181 tgctctgggc gcgctgggtt atgtggagag tgcgctggaa atcgcgcaac agtgtgaagg
    42241 ggcggttaat atttcgtcgg tggtggtcgc atcgggcagt gccggaactc acgccggatt
    42301 ggctgttggg ctggaacacc tgatgcctga aagcgaactg attggcgtga ccgtgtcgcg
    42361 ttccgttgcc gatcaattgc cgaaagtggt taacctacaa caggcgattg cgaaagaact
    42421 ggagctgacc gcatcagcgg aaattttact ctgggatgac tattttgcac ctggctacgg
    42481 cgtgccgaac gacgaaggca tggaagcagt gaaattgctg gcgcgtctgg aaggcattct
    42541 gcttgatcct gtgtataccg gaaaagcgat ggcggggctg attgacggta tcagtcagaa
    42601 acgcttcaaa gatgaagggc cgattctgtt tattcatacc ggcggcgcgc ctgcgctgtt
    42661 cgcctatcat ccccacgttt agctccggga acgctcatgc aagaaagtat acaactggtt
    42721 attgattcct tgccgttcct gttgaaaggg gctgagtata ccctgcaact cagtatcggc
    42781 ggcatgtttt ttggcttact actcgggttt attctcgcgc tgatgcgcct gtcgccaatc
    42841 tggcccgtgc gctggctggc gcgtttttat atctctattt ttcgtggtac gccgctcatc
    42901 gcacaactgt ttatgatcta ttacggcctg ccgcagtttg gtatcgaatt agatcccatt
    42961 ccgtcagcaa tgattggtct gtcgctgaac actgccgcct atgccgccga aacgctgcgc
    43021 gccgccattt cttctattga taaaggtcag tgggaagccg ctgccagtat tggtatgacg
    43081 ccgtggcaga ccatgcgtcg cgcgattttg ccacaggcgg cgcgggtggc gttgccgccg
    43141 ctgtcgaaca gctttatcag tctggtaaaa gatacgtcgc tggccgcgac aatccaggtg
    43201 ccggagctgt tccgtcaggc gcagttgatt acctcgcgca cgctggaggt gttcaccatg
    43261 tacctggcgg cttcgctcat ctactggatc atggcgacag tattatcgac gttgcagaac
    43321 cattttgaga atcaacttaa tcgccaggag agagaaccaa aatgagtgcc attgaagtta
    43381 agaacctggt gaaaaaattt cacggtcaga cggtgctgca cggtatcgac ctcgaggtaa
    43441 agcctggcga agtggtggca attatcggtc cgagtggttc cggcaaaacc acgttgctac
    43501 gcagcataaa tctgctggaa caaccggaag cgggaacgat caccgttggc gatatcacta
    43561 ttgatactgc acgttcatta agtcagcaaa aatcgctgat tcgccagttg cgtcagcacg
    43621 tcgggtttgt cttccagaac tttaatttgt ttccgcatcg tacggtgctg gaaaacatta
    43681 ttgaagggcc ggtgatcgtc aaaggcgaac cgaaagaaga ggccacggcg cgcgctcgcg
    43741 agctgctggc gaaagttggg ctggccggta aagaaaccag ctatccacgt cgtttgtctg
    43801 gcggtcaaca gcagcgtgtt gcgattgcgc gtgcactggc aatgcgtcct gaggtgattt
    43861 tgtttgacga gccaacgtcg gcgctggatc cagagctggt gggcgaagtc ctgaacacca
    43921 tccgccagct ggcgcaggaa aagcgcacga tggtgattgt gacacacgaa atgagctttg
    43981 cccgggatgt tgcggaccgg gcgatcttta tggaccaggg gcggatagtc gagcaggggg
    44041 cagcaaaagc gttatttgcc gatccccagc agcctcgcac ccgccagttc ctcgagaagt
    44101 ttctgctgca ataataagaa aaaatcagcc ccgacgattc acctgtcggg gcgggacgcc
    44161 atttcaagcg tgataaaact gcttaacaaa tcagcataac tcattaataa cataagagaa
    44221 tgcgatggct tgcaaaagta attcattgcc tgaataatat aaattatata taaatcttat
    44281 ttatgtgata gtttgaatta tcattataaa tgatactcac tctcaggggc gttgcggttt
    44341 actatgcagg atacggattt tttcagctgg cgtcgcacga tgctgttgcg ttttcagagg
    44401 atggaggccg cagaagaggt ctaccatgaa atagagcttc aggctcagca gctggagtac
    44461 gattactatt cgttatgtgt tcgccacccg gtaccattca cgcgacctaa agtggctttc
    44521 tacaccaatt accctgaggc gtgggttagt tattatcagg caaaaaactt tctcgcaatt
    44581 gatccggtgc tgaaccctga aaactttagt cagggccatt taatgtggaa tgatgactta
    44641 ttcagcgaag cacagccgtt atgggaagcc gcgcgcgcac atggtttacg ccgcggtgtc
    44701 actcagtatt taatgctgcc aaatcgggcg ctgggctttt tgtccttttc ccgttgcagc
    44761 acgcgcgaaa tacccattct tagtgatgaa ctgcaattaa aaatgcagtt actggtgcgc
    44821 gaaagtctga tggctctgat gcgtttaaat gatgaaatag tgatgacgcc agagatgaat
    44881 ttcagcaagc gcgaaaaaga aattctgaaa tggacggcgg aagggaagac atcagcagag
    44941 atagcgatga ttttgtcaat ctctgagaat acggtcaatt tccatcagaa aaacatgcag
    45001 aa
//
LOCUS       DAHBHC010000035        44559 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHBHC010000035 DAHBHC010000000
VERSION     DAHBHC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.035"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..857
                     /gene="fhuD"
                     /locus_tag="M2A35_002361"
     CDS             <1..857
                     /gene="fhuD"
                     /locus_tag="M2A35_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCB7198653.1"
                     /translation="LTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVP
                     YGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLA
                     RIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYEDFIRSMKPRFVKR
                     GERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAY
                     KDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVL
                     DNAIGGKA"
     gene            854..2836
                     /gene="fhuB"
                     /locus_tag="M2A35_002362"
     CDS             854..2836
                     /gene="fhuB"
                     /locus_tag="M2A35_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCB7198654.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ISVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(2871..4151)
                     /gene="hemL"
                     /locus_tag="M2A35_002363"
     CDS             complement(2871..4151)
                     /gene="hemL"
                     /locus_tag="M2A35_002363"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCB7198655.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            4376..5797
                     /gene="clcA"
                     /locus_tag="M2A35_002364"
     CDS             4376..5797
                     /gene="clcA"
                     /locus_tag="M2A35_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCB7198656.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5821..5886
                     /locus_tag="M2A35_002365"
     CDS             5821..5886
                     /locus_tag="M2A35_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198657.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            5879..6223
                     /gene="erpA"
                     /locus_tag="M2A35_002366"
     CDS             5879..6223
                     /gene="erpA"
                     /locus_tag="M2A35_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCB7198658.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(6270..6893)
                     /locus_tag="M2A35_002367"
     CDS             complement(6270..6893)
                     /locus_tag="M2A35_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCB7198659.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(6931..7731)
                     /gene="btuF"
                     /locus_tag="M2A35_002368"
     CDS             complement(6931..7731)
                     /gene="btuF"
                     /locus_tag="M2A35_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCB7198660.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(7724..8422)
                     /gene="mtnN"
                     /locus_tag="M2A35_002369"
     CDS             complement(7724..8422)
                     /gene="mtnN"
                     /locus_tag="M2A35_002369"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCB7198661.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            8506..10023
                     /gene="dgt"
                     /locus_tag="M2A35_002370"
     CDS             8506..10023
                     /gene="dgt"
                     /locus_tag="M2A35_002370"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCB7198662.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            10153..11577
                     /gene="degP"
                     /locus_tag="M2A35_002371"
     CDS             10153..11577
                     /gene="degP"
                     /locus_tag="M2A35_002371"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCB7198663.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            11732..12889
                     /gene="cdaR"
                     /locus_tag="M2A35_002372"
     CDS             11732..12889
                     /gene="cdaR"
                     /locus_tag="M2A35_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCB7198664.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGEQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTELDLGNFDDRLLLYVALQLDEER"
     gene            complement(12978..13364)
                     /locus_tag="M2A35_002373"
     CDS             complement(12978..13364)
                     /locus_tag="M2A35_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCB7198665.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(13676..14500)
                     /gene="dapD"
                     /locus_tag="M2A35_002374"
     CDS             complement(13676..14500)
                     /gene="dapD"
                     /locus_tag="M2A35_002374"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCB7198666.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(14531..17203)
                     /gene="glnD"
                     /locus_tag="M2A35_002375"
     CDS             complement(14531..17203)
                     /gene="glnD"
                     /locus_tag="M2A35_002375"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCB7198667.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(17265..18059)
                     /gene="map"
                     /locus_tag="M2A35_002376"
     CDS             complement(17265..18059)
                     /gene="map"
                     /locus_tag="M2A35_002376"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCB7198668.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            18427..19152
                     /gene="rpsB"
                     /locus_tag="M2A35_002377"
     CDS             18427..19152
                     /gene="rpsB"
                     /locus_tag="M2A35_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCB7198669.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            19410..20261
                     /gene="tsf"
                     /locus_tag="M2A35_002378"
     CDS             19410..20261
                     /gene="tsf"
                     /locus_tag="M2A35_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCB7198670.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            20408..21133
                     /gene="pyrH"
                     /locus_tag="M2A35_002379"
     CDS             20408..21133
                     /gene="pyrH"
                     /locus_tag="M2A35_002379"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCB7198671.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            21425..21982
                     /gene="frr"
                     /locus_tag="M2A35_002380"
     CDS             21425..21982
                     /gene="frr"
                     /locus_tag="M2A35_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCB7198672.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            22074..23270
                     /gene="ispC"
                     /locus_tag="M2A35_002381"
     CDS             22074..23270
                     /gene="ispC"
                     /locus_tag="M2A35_002381"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCB7198673.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            23459..24217
                     /gene="ispU"
                     /locus_tag="M2A35_002382"
                     /gene_synonym="uppS"
     CDS             23459..24217
                     /gene="ispU"
                     /locus_tag="M2A35_002382"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCB7198674.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            24230..25087
                     /gene="cdsA"
                     /locus_tag="M2A35_002383"
     CDS             24230..25087
                     /gene="cdsA"
                     /locus_tag="M2A35_002383"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCB7198675.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            25099..26451
                     /gene="rseP"
                     /locus_tag="M2A35_002384"
     CDS             25099..26451
                     /gene="rseP"
                     /locus_tag="M2A35_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCB7198676.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            26481..28913
                     /gene="bamA"
                     /locus_tag="M2A35_002385"
     CDS             26481..28913
                     /gene="bamA"
                     /locus_tag="M2A35_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCB7198677.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            29035..29520
                     /gene="skp"
                     /locus_tag="M2A35_002386"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             29035..29520
                     /gene="skp"
                     /locus_tag="M2A35_002386"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCB7198678.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            29524..30549
                     /gene="lpxD"
                     /locus_tag="M2A35_002387"
     CDS             29524..30549
                     /gene="lpxD"
                     /locus_tag="M2A35_002387"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCB7198679.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            30654..31109
                     /gene="fabZ"
                     /locus_tag="M2A35_002388"
     CDS             30654..31109
                     /gene="fabZ"
                     /locus_tag="M2A35_002388"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCB7198680.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            31113..31901
                     /gene="lpxA"
                     /locus_tag="M2A35_002389"
     CDS             31113..31901
                     /gene="lpxA"
                     /locus_tag="M2A35_002389"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCB7198681.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            31901..33049
                     /gene="lpxB"
                     /locus_tag="M2A35_002390"
     CDS             31901..33049
                     /gene="lpxB"
                     /locus_tag="M2A35_002390"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCB7198682.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            33046..33642
                     /gene="rnhB"
                     /locus_tag="M2A35_002391"
     CDS             33046..33642
                     /gene="rnhB"
                     /locus_tag="M2A35_002391"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCB7198683.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            33679..37161
                     /gene="dnaE"
                     /locus_tag="M2A35_002392"
     CDS             33679..37161
                     /gene="dnaE"
                     /locus_tag="M2A35_002392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCB7198684.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPQIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            37174..38133
                     /gene="accA"
                     /locus_tag="M2A35_002393"
     CDS             37174..38133
                     /gene="accA"
                     /locus_tag="M2A35_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCB7198685.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            38232..40373
                     /gene="ldcC"
                     /locus_tag="M2A35_002394"
     CDS             38232..40373
                     /gene="ldcC"
                     /locus_tag="M2A35_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCB7198686.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGISGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGKMLTKESRTVLDFLLMLCSV
                     VQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            40430..40819
                     /locus_tag="M2A35_002395"
     CDS             40430..40819
                     /locus_tag="M2A35_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCB7198687.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            40884..42182
                     /gene="tilS"
                     /locus_tag="M2A35_002396"
     CDS             40884..42182
                     /gene="tilS"
                     /locus_tag="M2A35_002396"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCB7198688.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(42231..42491)
                     /gene="rof"
                     /locus_tag="M2A35_002397"
     CDS             complement(42231..42491)
                     /gene="rof"
                     /locus_tag="M2A35_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCB7198689.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(42478..42678)
                     /locus_tag="M2A35_002398"
     CDS             complement(42478..42678)
                     /locus_tag="M2A35_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCB7198690.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            42844..43389
                     /locus_tag="M2A35_002399"
     CDS             42844..43389
                     /locus_tag="M2A35_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCB7198691.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSVFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            43386..43808
                     /gene="arfB"
                     /locus_tag="M2A35_002400"
     CDS             43386..43808
                     /gene="arfB"
                     /locus_tag="M2A35_002400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCB7198692.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            43822..44532
                     /gene="nlpE"
                     /locus_tag="M2A35_002401"
                     /gene_synonym="cutF"
     CDS             43822..44532
                     /gene="nlpE"
                     /locus_tag="M2A35_002401"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCB7198693.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        10526 a        10922 c        12388 g        10723 t
ORIGIN      
        1 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
       61 ctattgatcc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc
      121 tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac
      181 caccattgcc ggactcagtg atcgacgtcg gtttgcgcac agaacctaac cttgaactgc
      241 tgaccgaaat gaaaccatcg tttatggtct ggtcggcagg atatggccct tcaccagaaa
      301 tgctggctcg tattgcgccg gggcgtggat ttaacttcag cgacggcaaa cagccgctgg
      361 cgatggcgcg taagtcactg acggaaatgg cagatttact taacctgcaa agcgcagcag
      421 aaacgcattt agcgcactat gaagacttta tccgcagcat gaaaccccgc tttgtgaagc
      481 gtggtgaacg cccgttattg ctgacgacgc ttatcgatcc gcgccatatg ctggttttcg
      541 gtccaaacag cttgtttcag gaaattcttg atgagtacgg catcccaaat gcctggcaag
      601 gggaaaccaa cttctggggc agtaccgccg tcagcatcga tcgtctggcg gcgtataaag
      661 atgttgatgt gctctgtttt gatcacgaca acagcaaaga catggacgcg ctaatggcaa
      721 ccccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacccgcag
      781 tctggtttta tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg gataacgcca
      841 tcggaggtaa agcgtgagta aacgaattgc gctttttccg gcgttattgc tggcgctgct
      901 ggtgattgtc gctacggcgc tgacctggat gaacttttcg caggcgctgc cgcgtagcca
      961 gtgggcgcag gccgcctggt cgccggatat taacgtcatc gagcagatga tttttcacta
     1021 cagcttgtta ccacgtctgg cgatttctct actggtgggc gcgggcctgg ggctggtggg
     1081 cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc
     1141 tacaggcgcg caactgggga tttccgtcac cacgctctgg gcgatcccag gtgctatggc
     1201 gagccagttt gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc
     1261 gtgggggaaa cggctgtcgc cggtaacgct gattctcgcg gggctggtag tgagccttta
     1321 ttgtggggca atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt
     1381 cctgtggagc accggaacgc tgacgcaaac cgactggggc ggcgttgagc gtttatggcc
     1441 gcagttgctg ggcggcgtga tgctgacgtt actgctactt cgtccgttaa ccctgatggg
     1501 gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgctcgcgc gtctggcggc
     1561 gctgtcgctg gcgatagtca tcagtgcgct gctggtgaac gcagtgggga ttatcggctt
     1621 tatcgggttg ttcgcgccac tgctggcaaa aatgctgggg gcgcggcgtt tgttgccacg
     1681 gttgatgctg gcgtcgctga ttggtgcgtt aattctgtgg ctttctgatc aaatcatcct
     1741 ctggctgact cgcgtgtgga tggaagtttc caccggttcg gtcactgcgt tgatcggtgc
     1801 gccgctgcta ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt
     1861 taacgatcgt gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt
     1921 gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac
     1981 gtgggcgagc ggggcgctgc tcgatgattt aatgccctgg cgctggccgc gaattatggc
     2041 ggcgctgttt gcgggcgtca tgctggcggt agcgggctgt attattcagc ggctgaccgg
     2101 caacccgatg gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt
     2161 gttaatgctg tttctggtac cgggtaatgc ctttggctgg ctgttacccg caggcagtct
     2221 cggggcggcg gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc
     2281 acaccgcatg ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat
     2341 gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac
     2401 ctacaacgcg accgatgcgc aggtctggcg caccggaatt gtgatggtga ttttgctggc
     2461 gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcccg
     2521 agccgtagga atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct
     2581 gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca
     2641 tattgcgcgg atgatgggct ttcgacggac gatgccacac atcgtaattt cggcgctggt
     2701 gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca
     2761 gatcccggcg gggctgctgt caacctttat cggcgcgcca tattttatct atttgttgag
     2821 aaagcagagc cgttaattga atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc
     2881 gcaaacaccc gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg
     2941 gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata
     3001 tggaagaaac gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg
     3061 gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga
     3121 attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgatgt cagctcatcc
     3181 aacgtttcgt gaacgcccgg ttgcgcgact tcattcagac aggcgaaacc cgctgccatc
     3241 gcaattgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca
     3301 tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg
     3361 cccaggcagg tgagatccgg ttccacaccg taataatcct gtgcgccagc tagcgctacg
     3421 cggaagccag tcattacttc atcgatgatc agcaatgcgc caaattcgtc gcacagcgcg
     3481 cgcagacctg gcaggaactc tggcagcggt ggaacgcagt tcatattgcc tgccaccggc
     3541 tcgacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc
     3601 agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac acccggcgag
     3661 tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga
     3721 ccgtggtaac aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg
     3781 cggatggcgc tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc
     3841 gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag
     3901 cttaaaccac gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc
     3961 agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac
     4021 agataggcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg
     4081 cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca
     4141 gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta
     4201 ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt
     4261 tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg
     4321 aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa
     4381 aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgta gacaactgat
     4441 tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt
     4501 cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa
     4561 ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc
     4621 ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc
     4681 cgaagcaggg ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt
     4741 tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg
     4801 catggtgcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt
     4861 gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc
     4921 tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga
     4981 agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt
     5041 cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg
     5101 taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt
     5161 tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg
     5221 tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg
     5281 tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat
     5341 cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat
     5401 taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc
     5461 gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata
     5521 tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat
     5581 tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact accagctcat
     5641 tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa
     5701 accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc
     5761 gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata
     5821 atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat
     5881 gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag
     5941 cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg
     6001 ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac
     6061 catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg
     6121 cggttccgtt gattataccg aaggtctgga aggttctcgt ttcatcgtga ccaacccgaa
     6181 cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc
     6241 ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc
     6301 ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc
     6361 atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata
     6421 ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg
     6481 gctagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat gcaaaccgcg
     6541 attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc
     6601 agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc
     6661 agcacgatgg tcagcatgct ggtgaccatt gcaacgacca gatcggtggg atctttcacc
     6721 caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tactgcggtt
     6781 accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg
     6841 ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa
     6901 tccccactga gcacctgaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag
     6961 ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg
     7021 aataacggga attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg
     7081 tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg
     7141 cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg
     7201 gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac
     7261 acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat ccagcaggga
     7321 ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc
     7381 attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc
     7441 cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggctt
     7501 cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg
     7561 cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcagcaaa
     7621 ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag
     7681 tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt
     7741 tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat
     7801 cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg
     7861 ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg
     7921 cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc
     7981 cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg
     8041 cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt
     8101 cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat
     8161 cgcccacttt caacgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg
     8221 gcttgcagtg ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg
     8281 atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga
     8341 gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg
     8401 caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt
     8461 taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat
     8521 ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa
     8581 catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt
     8641 cgtcgtctgc aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg
     8701 cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta
     8761 agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct
     8821 tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt
     8881 cattttggcg aagcggcgat aaatgactgg tttcgccagc gtttataccc ggaagatgcc
     8941 gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg
     9001 gaagaaccgc ttaacgagtt gcggcgcaag attcgtcagg acttatgtca ttttgagggg
     9061 aatgcacaag gcattcgtct ggtgcataca ttgatgagga tgaatctcac ctgggcacag
     9121 gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca
     9181 catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg
     9241 ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa
     9301 gctgccgacg acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata
     9361 tttaccgttg agcagcttta tcatcatttg cacgaggcgt ggggtcagca tgagaaaggt
     9421 tcgctctttt cgctagtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc
     9481 cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta
     9541 ccatacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat
     9601 catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc
     9661 gctgtaaaac atgtgtttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg
     9721 gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt
     9781 actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg cttattccac
     9841 aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat
     9901 tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc
     9961 agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa
    10021 taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg
    10081 gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg
    10141 agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt
    10201 ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag
    10261 cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac
    10321 gtagaaggta gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc
    10381 ggtgatgatt ctccgttctg ccaggaaggt tcaccgttcc agagctctcc gttctgccag
    10441 gggggccagg gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc
    10501 gtcatcattg atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg
    10561 acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa
    10621 gatccgcgct ctgatatcgc gctgattcag atccagaacc cgaaaaacct gaccgcaatt
    10681 aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg
    10741 tttggcctgg gagagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg
    10801 aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc
    10861 ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca
    10921 ccggacggcg gcaacatcgg tatcggtttt gctatcccga gcaacatggt gaaaaacctg
    10981 acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact
    11041 gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta
    11101 agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc
    11161 acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca ggtgggtact
    11221 atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg
    11281 aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc
    11341 attgaaggtg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg
    11401 aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg
    11461 aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct
    11521 gtgctggcac tgaacattca gcgcggcgac agctccatct acctgttaat gcagtaatct
    11581 ccctcaaccc cttcctgaaa acgggaaggg gttctcctta caatctgtga acttcaccac
    11641 aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt tacgtacttc
    11701 cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat
    11761 ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga
    11821 tgcccgtggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg
    11881 tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg tggcacgtca
    11941 tctgcacggt gtgcggcaag ggattaatct accgttacgg ctggaaggtg aaattgtcgg
    12001 cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat
    12061 gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ttgttggctc aggatagccg
    12121 tttgcgggag gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttac
    12181 tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtggtgg ctattgttga
    12241 ggtcgacagc ggtcagcttg gtgtggacag cgcaatggcg gagttacaac aactgcaaaa
    12301 cgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt
    12361 ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg
    12421 agttgaacaa ctgattaccc gcatgaaaga gtacggccag ctgcgttttc gcgtttcact
    12481 gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac
    12541 gatggtggtg ggtgaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat
    12601 gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaacgaac tggcgcgacc
    12661 gctggcgcgg ctgaaagcga tggacaataa cggcttgctg cgacgaacgc tggcggcgtg
    12721 gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc atcgtaatac
    12781 tctggagtat cggcttaatc gtatatcgga actgaccgag cttgatttgg gtaattttga
    12841 tgacaggttg ctgctgtatg tggcgttgca gctggatgaa gagcggtagg ttatgcgtta
    12901 aggtcgaggc ggcgctggct cattcccctc agcccgaagg gtagagggga atgtccgagt
    12961 taaccgtcag ttcggcatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg
    13021 atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga
    13081 tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga
    13141 ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt
    13201 ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc
    13261 tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt
    13321 tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat
    13381 gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc
    13441 gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct
    13501 ttattgtggc gatattccat gggatggatt agcacattta tcttcgtttt gcgaattaca
    13561 tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg gggaagaatg agtaggtcga
    13621 attattctga gccagaaagc atacgggcag cattatgctg cccgtatatg tactcttaat
    13681 cgatggtacg cagcagttcg ttaatgccga ctttgccgcg agttttcgcg tcaactttct
    13741 taacgataac cgcacagtag aggctgtatt tgccatcttt cgacggcaga ttacctgaaa
    13801 caaccacaga ccccgccgga acgcgaccgt agtggatttc gccggtttca cggtcgtaaa
    13861 tacgggtgct ctgaccaatg tatacgccca tagagatgac ggaaccttct tcgacaatca
    13921 ccccttcaac cacttcggag cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt
    13981 tagcctgcag cggctccagc acgccgccga tgcccacgcc accggaaagg tggacgtttt
    14041 taccaatctg cgcacaagaa ccgacggtcg cccaggtatc aaccatcgtg ccttcatcaa
    14101 catatgcgcc gatgttgacg taagacggca tcagcacggt gttacgggca ataaatgcac
    14161 cctgacgtac cgccgctggt ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt
    14221 cgtagtcggc gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca
    14281 cctgattatc attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca
    14341 cccactgacc gtcaattttt tccgctacac gcagtgcacc ggaatccagc agggcgatca
    14401 cctgatttac cgcttcgcgg gtaacggtgt ctgcgttggc tggcgtgatc tcggcacggc
    14461 gttcaaaagc ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc
    14521 agtaaacaca tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt
    14581 cctgctgcaa ctcgttatta agtgcacgcc ggtcagcggt ggcaattatg aataaatctt
    14641 ctactcgctc gccaatggtt gtaattcggg caccatgaag cgaaattccc agatcggcaa
    14701 aaattttccc gactctcgcc agcagtcctg gctggtcgag cgcgataagc tcaaggaacg
    14761 atttgcggtc ggtatgggtc ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta
    14821 atttggcggg ttggcgacgg ggctgcggtg gctgccagct actttgcgtc agtacttgct
    14881 ccagaccaaa ccgaataacc tcatgacgat ctgcggacag cgggctgcca tcgggttcca
    14941 gcacgataaa ggtatccatc gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa
    15001 cacttaaatt gcggcggtct aattcggcac agacggcggc aaacagatag gggcggtccg
    15061 ggctccagat aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc aataccagcg
    15121 gtttgcttaa atcatgctgt aataaatggc gggcatgcca ggccagttga tttgggctat
    15181 ggcggacaaa atagttagca cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga
    15241 tgttatccat gcgcagtagt gccagtgcct ggagttggtg atggcgaacc cgttcgcgca
    15301 tatccggcgt gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc
    15361 gcaacagact ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca
    15421 ctgtcaggca taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct
    15481 tgatgacttc agggtcctga atatcgcggc gttgtgctgt caccgacatc agcaagtgct
    15541 ggcgaaccag ccaggcaacc agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa
    15601 aatgcactac atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat
    15661 cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg caggcgcggc cagacgtcca
    15721 cacacaacgg atggcgctgg cgcgtttctt cactggcaaa actctccagt ttcagcatca
    15781 cgcggatagt atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga
    15841 cgatatgcga ccattgcggc atatacgcgc cgagcacgct atggcgatgc attggcaata
    15901 gcccgcgccg caccgctccg gggtgacgca gaatgctcaa aaacagtttt cgtgcttccg
    15961 gaatattaca cagcggttgt tgcagatggc gacgggcatg gcgtaactgg cgcagcgtgg
    16021 tggagtaaat gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg
    16081 cttccggctg gcgcataaat agtgtttcat cacgcaggtc gattagcgta ccgcgtagct
    16141 gaaactcatc gtcgattgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat
    16201 cgaacagttg cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat
    16261 ccttcatcat ccgctcgacc ggttcgttgc cttcaccact gtaattcaga cgctgagcga
    16321 cgctaagttg gcgatcgaat aacagacgat tatcgtaacg gctgacgacc agatgcaggg
    16381 caaagcgaat acgccacagt atatgcagac attcgtttaa ttccgcccgc tccgctgagg
    16441 ttaagaagcc aaacccaacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca
    16501 cccattgcag agtgtggata tcgcgcaagc cgccagggct acttttgatg tctggttcaa
    16561 gattgtagct ggtgccgtgg taacgctgat ggcgctggtt ctgttcttca actttcgccg
    16621 cgtagaactt gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc agttcgagga
    16681 agagcgcaac atcgccaatt aataagcggg attcgattaa attggtggcg acggttaaat
    16741 ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac actatgaccg acttccagct
    16801 ttacatccca gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct
    16861 ttttacggct taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt
    16921 agccaccgac ggcgaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata
    16981 atcgttgcag gagctggtcg ataaactcgg tgcgcgcctc aatcaactgt tctgcagaga
    17041 tcccattgtc aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc
    17101 caccgacggt taattcatca cggggccaga cgcatggatt ttgcggttga ccgggcaggg
    17161 tggggagagc ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag
    17221 attaaattat cgcattaaaa aagccggcat tcgccggctt catcttattc gtcgtgcgag
    17281 attatcgccg ggatggtgtc atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc
    17341 accacaatag tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag
    17401 ccatctttca tggtgcggat ctctttttta cccgcgttga ccattggctc aatggtgaac
    17461 gtcatccccg gtttcagcac gacgttggtt tcacgggagt catagtgcag cacctgcggt
    17521 tcttcatgga agccgcgacc aataccgtgt ccacaatatt cacgaacgac ggagaagcct
    17581 tctgcttcga caaacttctg aatcgccgca ccgatttcgc gcaggttaat gcctggtttg
    17641 accatgcgca gcgccaggta caggctttct tgcgtgatgc ggcacagacg ttcgcccatg
    17701 atggtcggct taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt
    17761 acggtgacat caatgttaac gatatcgcca tctttcagca gcttagcatc gtccgggata
    17821 ccgtggcaca ccacttcatt aatagagatg caaacggatt tcggatagcc gtgatagccg
    17881 aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccagc
    17941 tcgccggtgc tgacgcccgg tttaacatac ggttcgatca tctccagcac ttcggcagcc
    18001 agtcggccag cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt
    18061 aattctgtcc atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat
    18121 cacacccggg caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc
    18181 tataatagaa aattcgacat ctgatgctgt acacagcgcc aacaattatt ggtgtccatg
    18241 acgtatttgt ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac
    18301 ggaagcgaca atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc
    18361 cctttggggt cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt
    18421 ttaatcatgg caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac
    18481 cagacccgtt actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt
    18541 cacatcatca accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac
    18601 aagattgctt ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa
    18661 gcggtgaaag acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc
    18721 ggtatgctga ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa
    18781 actcagtctc aggacggtac tttcgaaaag ctgaccaaga aagaagcgct gatgcgcact
    18841 cgtgagctgg agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg
    18901 gacgctctgt ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac
    18961 ctgggtattc cggtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc
    19021 gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct
    19081 gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta
    19141 gaagctgagt aataaggctt gataactccc ccaaaatagt tcgagttgca gaaaggcggc
    19201 aagctcgaga attcccggga gcttacatca gtaagtgacc gggatgagcg agcgaagata
    19261 acgcatctgc ggcgcgaaat atgaaggggg agagccctta tagaccaggt agtacacgtt
    19321 tggttagggg gcctgcatat ggcccccttt ttcactttta tatctgtgcg gtttaatgcc
    19381 gggcagatca catctccgag gattttagaa tggctgaaat taccgcatcc ctggtaaaag
    19441 agctgcgtga gcgtactggc gcaggcatga tggattgcaa aaaagcactg actgaagcta
    19501 acggcgacat cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga
    19561 aaaaagcagg caacgttgct gctgacggcg tgatcaaaac caaaatcgac ggcaactacg
    19621 gcatcattct ggaagttaac tgccagactg acttcgttgc aaaagacgct ggtttccagg
    19681 cgttcgcaga caaagttctg gacgcagctg ttgctggcaa aatcactgac gttgaagttc
    19741 tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt gaaaacatca
    19801 acattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag cacggtgcgc
    19861 gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta
    19921 tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga agacgtatcc gctgaagtgg
    19981 tagaaaaaga ataccaggta cagctggata tcgcgatgca gtctggtaag ccgaaagaaa
    20041 tcgcagagaa aatggttgaa ggccgcatga agaaattcac cggcgaagtt tctctgaccg
    20101 gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg
    20161 ctgaagtgac tggcttcatc cgcttcgaag tgggtgaagg catcgagaaa gttgagactg
    20221 actttgcagc agaagttgct gcgatgtcca agcagtctta attatcgaaa aggagccgcc
    20281 tgagggcggc ttctttttgt gcccatcttg taaattcagc taacccttgt ggggctgcgc
    20341 tgaaaagcga cgtacaatgt cgctagtatt aattcatttc aatcgttgac agtctcagga
    20401 aagaaacatg gctaccaatg caaaacccgt ctataaacgc attctgctta agttgagtgg
    20461 cgaagctctg cagggcactg aaggcttcgg tattgatgca agcatactgg atcgtatggc
    20521 tcaggaaatc aaagaactgg ttgaactggg tattcaggtt ggtgtggtga ttggtggggg
    20581 taacctgttc cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca
    20641 catggggatg ctggcgaccg taatgaacgg cctggcaatg cgtgatgcac tgcaccgcgc
    20701 ctatgtgaac gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg acagctacag
    20761 ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac
    20821 aggtaacccg ttctttacca ccgactcagc agcttgcctg cgtggtatcg aaattgaagc
    20881 cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc cggcgaaaga
    20941 tccaaccgca accatgtacg agcaactgac ttacagcgaa gtgctggaaa aagagctgaa
    21001 agtcatggac ctggcggcct tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt
    21061 caacatgaac aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt
    21121 aatcacggaa taattcccgt gatggataaa taagggtaag attccgcgta agtatcgcgg
    21181 gggcgttagt ctggttataa ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc
    21241 agcaaccgga gcgtacaaaa gtacgtgagg atggcgagca ctgcccgggg ccaaaatggc
    21301 aaataaaata gcctaataat ccagacgatt acccgtaata tgtttaatca gggctatact
    21361 tagcacactt ccactgtgtg tgactgtctg gtctgactga gacaagtttt caaggattcg
    21421 taacgtgatt agcgatatca gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc
    21481 gttcaaaacc caaatcagca aaatacgcac gggtcgtgct tctcccagcc tgctggatgg
    21541 cattgtcgtg gaatattacg gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt
    21601 agaagattcc cgtacactga aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga
    21661 aaaagcgatt atggcgtccg atcttggcct gaacccgaac tctgcgggta gcgacatccg
    21721 tgttccgctg ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg
    21781 tgaagcagaa caagcgcgtg ttgcagtacg taacgtgcgt cgtgacgcga acgacaaagt
    21841 gaaagcactg ttgaaagata aagagatcag cgaagacgac gatcgccgtt ctcaggacga
    21901 tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga
    21961 agcagaactg atgcagttct gatttcttga acgacaaaaa cgccgctcag tagatccttg
    22021 cggatcggct ggcggcgttt tgctttttat tctgtctcaa ctctggatgt ttcatgaagc
    22081 aactcaccat tctgggctcg accggctcga ttggttgcag cacgctggac gtggtgcgcc
    22141 ataatcccga acacttccgc gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg
    22201 tagaacagtg cctggaattc tctccccgct atgccgtaat ggacgatgaa gcgagtgcga
    22261 aacttcttaa aacgatgcta cagcaacagg gtagccgcac cgaagtctta agtgggcaac
    22321 aagcagcttg cgatatggca gcgcttgagg atgttgatca ggtgatggca gccattgttg
    22381 gcgctgctgg gctgttacct acgcttgctg cgatccgcgc gggtaaaacc attttgctgg
    22441 ccaataaaga atcactggtt acctgcggac gtctgtttat ggacgccgta aagcagagca
    22501 aagcgcaatt gttaccggtc gatagcgaac ataacgccat ttttcagagt ttaccgcaac
    22561 ctatccagca taatctggga tacgctgacc ttgagcaaaa tggcgtggtg tccattttac
    22621 ttaccgggtc tggtggccct ttccgtgaga cgccattgcg cgatttggca acaatgacgc
    22681 cggatcaagc ctgccgtcat ccgaactggt cgatggggcg taaaatttct gtcgattcgg
    22741 ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt aacgccagcg
    22801 ccagccagat ggaagtgctg attcacccgc agtcagtgat tcactcaatg gtgcgctatc
    22861 aggacggcag tgttctggcg cagctggggg aaccggatat gcgtacgcca attgcccaca
    22921 ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa
    22981 gtgcgttgac atttgccgca ccggattatg atcgttatcc atgcctgaaa ctggcgatgg
    23041 aggcgttcga acaaggtcag gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg
    23101 ttgctgcttt tcttgcgcaa caaatccgct ttacggatat cgctgcgttg aatttatcgg
    23161 tactggaaaa aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg
    23221 cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga ggataatccg
    23281 gctacagaga gtcgcgctat ttgttagcgt agggcttcag tgatatagtc tgcgccatct
    23341 gatcgtaagt agttggcttt ataaggtcag gtatgccgtg gttttacacg gctttttttt
    23401 gtataggctt cagtattcct gagtaccgta aaccctgtca gggaataaaa aacgcgtgat
    23461 gttgtctgct actcaaccac ttagcgaaaa attgccagcg catggctgcc gtcatgttgc
    23521 gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg
    23581 gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct tttgcggcca acaacggtat
    23641 tgaggcgtta acgctgtatg cctttagtag tgaaaactgg aaccgaccag cgcaggaagt
    23701 cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg
    23761 acataacgtg cgtctgcgta ttattggcga taccagtcgc tttaactcgc gtttgcaaga
    23821 acgtattcgt aaatctgaag cgctaacagc cgggaatacc ggtctgacgc tgaatattgc
    23881 ggcgaactac ggtggacgtt gggatatagt ccagggagtc aggcaactgg ctgaaaaggt
    23941 gcagcaagga aacctgcaac cagatcagat agatgaagag atgctaaacc agcatgtctg
    24001 tatgcatgaa ctggcccctg tagatttagt aattaggact gggggggagc atcgcattag
    24061 taactttttg ctttggcaaa ttgcctatgc cgaactttac tttacagatg ttctctggcc
    24121 cgatttcgat gaacaagact ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg
    24181 tttcggcggc accgagcccg gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta
    24241 tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc
    24301 gccggtgggg ttcgccatcg taacgctggt ggtctgcatg ctggcagcgt gggaatgggg
    24361 acagcttagc ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt
    24421 attgttggcg ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc atcaaccgct
    24481 ggttgaaatc tcactttggg cttcgctggg ttggtggatt gtcgcgctat tgctggtgct
    24541 gttttaccca ggttccgcag caatctggcg taactctaaa acattgcgcc ttatttttgg
    24601 cgtgctaacc attgttccct tcttctgggg catgctggcg ttacgggcct ggcactacga
    24661 cgagaatcat tacagtggcg caatatggct gctctatgtc atgatcctgg tatggggcgc
    24721 tgactctggc gcatatatgt ttggcaaatt gtttggtaaa cataagctgg caccgaaggt
    24781 ttctccgggt aaaacctggc aaggctttat aggtggacta gcgaccgcag cggtaatctc
    24841 atggggttat ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac tcatttgctc
    24901 tattgtcgca gcgttagcct cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga
    24961 agcaggaatt aaggacagcg gtcatttaat tccaggacac ggtggtattt tagatcgtat
    25021 tgatagcctg acggctgcgg taccggtctt tgcttgcttg ttgttactgg tattcaggac
    25081 gctttaacgg aaggtaatat gctgagtttt ctctgggatt tggcttcgtt catcgttgca
    25141 ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct gggttgcccg gcgttgtggt
    25201 gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc tctggcggcg aactgataag
    25261 ctcggcaccg aatatgttat cgccctgatc ccgctgggcg gttatgtcaa aatgctggat
    25321 gagcgcgcag aaccggtcgt tccggaactc cgccaccatg ccttcaataa taaatctgtt
    25381 ggccaacgag cggcgattat tgccgcaggt ccggttgcaa acttcatttt tgctatcttt
    25441 gcctactggc tggtttttat tattggtgtg cctggcgtac gtccggtggt tggtgaaata
    25501 gcagccaatt cgatagctgc ggaagcacaa attgcaccag gtacggaact aaaagccgta
    25561 gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga taaaattggc
    25621 gatgaaagca ccaccattac agtagcgcca tttggcagcg atcaacggcg ggatgtaaag
    25681 ctcgatttac gtcactgggc gtttgagcct gataaagaag atccggtatc ttcgctgggg
    25741 attcgtcctc gtgggccgca aattgaacct gtactggaaa atgtgcagcc aaactcggcg
    25801 gcaagcaagg caggtttgca agcaggcgac aggatcgtta aagtcgatgg tcagccctta
    25861 acgcagtggg tgacctttgt gatgcttgtc cgggataacc cgggtaaatc cttagcgtta
    25921 gaaatcgaaa ggcagggaag tcccttgtct ttgacattaa tcccggagag taaaccgggt
    25981 aatggtaaag cgattggttt tgtcggtatt gagccgaaag tcattccttt gccagatgag
    26041 tataaagttg tacgccagta tgggccgttc aacgccatcg tcgaagccac ggacaaaacg
    26101 tggcagctga tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa
    26161 ctgaacaacc tcagtgggcc gatctctatc gccaaggggg ctgggatgac agcggaactc
    26221 ggggtagttt attacctgcc gtttcttgcg cttattagcg tgaacttagg gataattaac
    26281 ctgtttccgt tgcccgtact tgacgggggg catctgctgt tccttgcgat cgaaaagatc
    26341 aagggcggac cggtatccga gcgggttcaa gacttttgtt atcgcattgg ctcgattctg
    26401 ctggtgctgt taatggggct tgcacttttc aatgatttct ctcggttatg agagttagtt
    26461 aggaagaacg cataataacg atggcgatga aaaagttgct catagcgtcg ctgctgttta
    26521 gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc
    26581 ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc ggtgcgcaca ggcgacacgg
    26641 ttaatgatga agatatcagt aataccattc gcgctctgtt tgctaccggc aactttgagg
    26701 atgttcgcgt ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa cgtccgacca
    26761 ttgccagcat tactttctcc ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc
    26821 tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt gccgatatcg
    26881 agaaaggtct ggaagacttc tactacagcg tcggtaaata tagcgccagc gtaaaagctg
    26941 tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt
    27001 cagctgaaat ccagcaaatt aacattgttg gtaaccatgc tttcaccacc gacgaactga
    27061 tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa cgtggtaggc gatcgtaaat
    27121 accagaaaca gaaactggcg ggcgaccttg aaaccctgcg cagctactat ctggatcgcg
    27181 gttatgcccg tttcaacatc gactctaccc aggtcagtct gacgccagat aaaaaaggta
    27241 tttacgtcac ggtgaacatc accgaaggcg atcagtacaa gctttctggc gttgaagtga
    27301 gcggcaacct tgccgggcac tccgctgaaa ttgagcagct gactaagatc gagccgggtg
    27361 agctgtataa cggcaccaaa gtgaccaaga tggaagatga catcaaaaag cttctcggtc
    27421 gctatggtta tgcctatccg cgcgtacagt cgatgcccga aattaacgat gccgacaaaa
    27481 ccgttaaatt acgtgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt
    27541 ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag
    27601 gtgcatggct ggggagcgat ctggtcgatc agggtaagga gcgtctgaat cgtctgggct
    27661 tctttgaaac tgtcgatacc gatacccaac gtgttccggg tagcccggac caggtagatg
    27721 tagtctacaa ggtgaaagaa cgtaacaccg gtagcttcaa cttcgggatt ggttacggta
    27781 ctgaaagtgg cgtgagcttc caggctggtg tgcagcagga taactggtta ggtacaggtt
    27841 atgctgttgg tatcaacggg accaaaaacg attaccagac ctatgctgaa ctgtcggtaa
    27901 ccaacccgta cttcactgta gatggtgtaa gcctcggtgg tcgtctcttc tataatgact
    27961 tccaggcaga tgacgccgac ctgtccgact ataccaacaa gagttatggt acagacgtga
    28021 cgttgggctt cccgatcaac gaatataact cgctgcgtgc aggtctgggt tatgtacata
    28081 actccctgtc caacatgcag cctcaggttg cgatgtggcg ttatctgtac tctatgggtg
    28141 aacatccgag cacctctgat caggataata gcttcaaaac ggacgacttc acgttcaact
    28201 atggttggac ctataacaag cttgaccgtg gttacttccc gacagatggt tcacgtgtca
    28261 acctgaccgg taaagtgacc attcctggat cggataacga atactacaaa gtgacgttag
    28321 acacggcgac ttatgtgccg atcgatgacg atcacaaatg ggttgttctg gggcgtaccc
    28381 gctggggtta tggtgatggt ttaggcggca aagagatgcc gttctacgag aacttctatg
    28441 ccggtggttc cagcaccgtg cgtggcttcc agtccaatac aattggtccg aaagcagttt
    28501 acttcccgca tcaggccagt aattatgatc cggactatga ttacgaatgt gcgactcagg
    28561 acggcgcgaa agacctgtgt aaatcggatg atgctgtagg cggtaacgcc atggcggttg
    28621 ccagcctcga gttcatcacc ccgacgccgt ttattagcga taagtatgct aactcggttc
    28681 gtacttcctt cttctgggat atgggtaccg tttgggatac aaactgggat tccagccaat
    28741 attctggata tccggactat agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat
    28801 tacaatggat gtccccattg gggccgttgg tgttctccta cgcccagccg ttcaaaaagt
    28861 acgatggaga caaggcagaa cagttccagt ttaacatcgg taaaacctgg taagtgttct
    28921 ccacaaagga atgtagtggt agtgtagcga tgactttagg cgatcaatat aagatcgccg
    28981 ggccacgcaa agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa
    29041 aagtggttat tagctgcagg tctcggttta gcactggcaa cttctgctca ggcggctgac
    29101 aaaattgcaa tcgtcaacat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt
    29161 tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc
    29221 gatctgcagg ctaaaatgaa aaagctgcag tccatgaaag cgggcagcga tcgcactaag
    29281 ctggaaaaag acgtgatggc tcagcgccag acttttgctc agaaagcgca ggcttttgag
    29341 caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac tggttactcg tatccagact
    29401 gctgtgaaat ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt
    29461 gcttacaaca gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa
    29521 gtaatgcctt caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt
    29581 gatggcgata tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt
    29641 acgttcatgg ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt
    29701 gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc
    29761 tacctgactt acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac
    29821 attgcaccca gtgcggtgat cgacgcgacg gcaaagctgg gtaacaacgt atcgattggc
    29881 gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata acgtgattat cggtgccggt
    29941 tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc
    30001 atttaccatg agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc
    30061 gcagacggct ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt
    30121 cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca caaccatcga tcgcggcgcg
    30181 ctggatgaca ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat
    30241 aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg
    30301 aaaattggtc gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata
    30361 tgcgacaaag tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc
    30421 gtctattcct caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg
    30481 gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa
    30541 caagactaac gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt
    30601 gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta
    30661 ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt
    30721 tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa
    30781 atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg
    30841 gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag
    30901 gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc
    30961 gcccggtcgt gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg
    31021 gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc gaagcaacga
    31081 tgatgtgtgc tcgtagccgg gaggcctgat aagtgattga taaatccgcc tttgtgcatc
    31141 caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt
    31201 gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg
    31261 tgaatggtca tactaaaatt ggccgcgata atgagattta tcagttcgcc tccatcggcg
    31321 aagttaacca ggatctgaaa tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta
    31381 accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga
    31441 aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag
    31501 gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact
    31561 tcgcgatcat cggcggcatg accgcagtcc atcagttctg catcattggt gcgcacgtga
    31621 tggttggcgg ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta
    31681 accacgcaac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg
    31741 aggcgattac cgctatccgc aatgcgtata agctgattta tcgtagcggt aaaacgctcg
    31801 atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta
    31861 ccgatttctt tgcacgctca acgcgcggtc tgattcgtta atgactgaac agcgtccatt
    31921 aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg
    31981 cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca
    32041 ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga
    32101 agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg
    32161 cgaactgaag ccagatattt ttgttggtat tgatgcgcct gacttcaata ttactcttga
    32221 aggtaacctc aaaaagcagg gtatcaaaac cattcattac gtcagtccat ccgtctgggc
    32281 gtggcgacag aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct
    32341 gcctttcgaa aaagcgtttt atgacaaata caatgtaccg tgccgcttta tcggtcatac
    32401 catggctgat gccatgccgt tagatccaga taaaaatgcc gcccgtgatg tgctggggat
    32461 ccctcacgat gcccactgcc tggcgttgct accagggagc cgtggtgcag aagttgaaat
    32521 gcttagtgcc gatttcctga aaacggccca gcttttgcgc cagacatatc cggatctcga
    32581 aatcgtggtg ccactggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctga
    32641 agtcgcgcca gacctttcag ttcatttgct ggatgggatg ggccgtgagg cgatggtcgc
    32701 cagcgatgcg gcgctactgg cgtcgggtac ggcagccctg gagtgtatgc tggcgaaatg
    32761 cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt
    32821 gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagaatt
    32881 attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa
    32941 cgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg
    33001 caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtt
    33061 tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgtgggcc gttagttggc
    33121 gcggtcgtca ccgctgcggt gatccttgac ccggcgcgcc cgattgccgg gctgaatgat
    33181 tccaaaaagc tgagcgaaaa acgccgtctg gcgctctatg aagagatcaa agagaaagcg
    33241 ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa cattcttcat
    33301 gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg
    33361 ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggc
    33421 gatagccgcg taccggaaat cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc
    33481 gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca caaagggtac
    33541 ccaaccgctt ttcatctgga aaaactggct gaacacggcg cgaccgaaca ccatcggcgc
    33601 agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa
    33661 gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac
    33721 tactcgatga tcgatggcct ggccaaaacc gcaccgttgg taaaaaaggc ggcggcgttg
    33781 ggtatgccag cactggcgat caccgatttc accaaccttt gtggtctggt gaagttctac
    33841 ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa cgtccagtgc
    33901 gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat
    33961 cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg
    34021 atcatcgatc gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcgga
    34081 cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt
    34141 gtcgcgtttt atgaagaaca cttcccggat cgctattttc ttgagctgat ccgcaccggc
    34201 aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg
    34261 cccgtcgtgg cgaccaacga cgtgcgcttt atcgacagca gcgactttga cgcacacgaa
    34321 atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat
    34381 tcgccgcagc aatatatgcg tagcgaagag gagatgtgtg agctgtttgc cgacatcccc
    34441 gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaaccgt gcgtcttggt
    34501 gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag
    34561 cgtgcaaaag agggcctgga agagcgtctg gcctttttat tccctgatga ggaagaacgt
    34621 cttaagcgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag
    34681 atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac
    34741 ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtggc ctacgcgctg
    34801 aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg
    34861 gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt
    34921 atcgagcacg tagcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt
    34981 acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc
    35041 tttgtcgatc gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg
    35101 tttgaagccg agccgcagct gccgcaaatc tacgaagcgg atgaagaagt taaggcgctg
    35161 atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg
    35221 gtggttatcg caccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc
    35281 aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc
    35341 gacttccttg gtttgcgtac gctcaccatc atcaactggg cgctggagat gatcaacaag
    35401 cggcgggcga agaatggcga gccgccgctg gatatcgccg cgatcccgct ggatgacaag
    35461 aaaagcttcg acatgctgca acgctcggaa accactgcgg tattccagct tgaatcgcgc
    35521 ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgccctg
    35581 gtggcactgt tccgccccgg tccgttgcaa tcagggatgg tggataactt tatcgaccgt
    35641 aaacatggtc gtgaagagat ctcctatccg gacgtgcagt ggcagcatga aagcttgaaa
    35701 ccggtactgg agccaaccta cggcatcatc ctgtatcagg aacaggtcat gcagattgcc
    35761 caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc gatggggaag
    35821 aaaaagccgg aagagatggc taagcaacgt tctgtatttg ctgaaggtgc agaaaagaac
    35881 ggaatcaacg ctgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgctggttac
    35941 ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac gttatggctg
    36001 aaagcacact atcctgcgga gtttatggcg gcggtaatga ccgccgatat ggacaacacc
    36061 gagaaggtgg tgggtctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca
    36121 gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt
    36181 attggcgcga tcaaaggggt cggtgaaggt ccgattgagg ccatcatcga agcccgtaat
    36241 aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac caaaaagttg
    36301 aaccgtcgcg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacat
    36361 cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa
    36421 gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt
    36481 gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt
    36541 gaaacgttag gcctgtacct gaccggacac cctatcaacc agtatttaaa agagattgag
    36601 cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc
    36661 acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc
    36721 ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc
    36781 ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc
    36841 agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac
    36901 gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat
    36961 gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca
    37021 gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg
    37081 cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag
    37141 gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa
    37201 cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat
    37261 gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa
    37321 ctgacacgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat
    37381 ccacagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg
    37441 gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtcttgac
    37501 ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc
    37561 cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg
    37621 gctgaacgct ttaagatgcc aatcatcacc tttatcgaca ccccgggggc ttatccgggc
    37681 gtgggcgcag aagagcgcgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct
    37741 cgccttagcg taccgactat ttgtaccgtt atcggtgaag gtggttctgg cggcgcgctg
    37801 gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg
    37861 ccggaaggtt gtgcgtccat tctgtggaag agcgctgata aagcgccgct ggcggctgaa
    37921 gcgatgggta tcatcgctcc gcgtctgaaa gaactaaaac tgatcgactc catcatcccg
    37981 gaaccgctgg gtggtgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa
    38041 ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt
    38101 tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac
    38161 ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc
    38221 aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg
    38281 agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac
    38341 aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt
    38401 ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt aatgaatatc
    38461 tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc
    38521 ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta
    38581 tgcgtcagta caccgacgaa tatcttgata acattacacc gccgttcacg aaagccttgt
    38641 ttacctacgt caaagagcgg aagtacacct tttgtacgcc ggggcatatg ggcggcaccg
    38701 catatcaaaa aagcccggtt ggctgcctgt tttatgattt tttcggcggg aataccctca
    38761 aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac accgggccac
    38821 acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta
    38881 ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta
    38941 cgctgttgat cgaccgcaat tgtcataaat cgctggcgca tctgttgatg atgaacgatg
    39001 tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattcttggt gggatcccgc
    39061 gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccaccacg caggcacaat
    39121 ggccggttca tgcggtgatt accaactcca cctatgatgg cttgctctac aacaccgact
    39181 ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtaca
    39241 cccattttca tccgatctac caggggaaaa gtggtataag cggcgagcgt gttgcgggaa
    39301 aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcgc
    39361 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
    39421 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc
    39481 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
    39541 aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
    39601 cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg cacggcttta
    39661 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgacaccgg
    39721 ggatggacga acagggcaat atgagcgagg aggggatccc ggcggcgctg gtggcaaaat
    39781 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
    39841 ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
    39901 aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc tatgctgaag
    39961 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
    40021 ttcgtaaaca cgatcttccc ggtttgatgt tgcgggcatt cgatactttg ccggaaatga
    40081 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
    40141 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
    40201 cgctgttgat gccaggaaaa atgctgacca aagagagccg cacggtactc gattttctac
    40261 tgatgctttg ttccgtagtg caacattacc ccggttttga aacggatatt cacggcgcga
    40321 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
    40381 gagcggcttc cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
    40441 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
    40501 ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
    40561 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
    40621 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
    40681 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
    40741 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
    40801 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
    40861 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
    40921 gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
    40981 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
    41041 taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
    41101 tgccgctggt ggtcgaacgc gtgcaacttg cgcaagaagg actgggcatt gaggcccagg
    41161 cgcggcaggc gcgttatcag gcatttgccc gcaccttgtt gcccggtgaa gtgctggtca
    41221 ccgcgcaaca tctcgacgat caatgtgaaa cttttctgct ggcgctaaaa cgcggtagcg
    41281 gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca cggcttatcc
    41341 gcccgttgct cgcccgcacg cggggagaac tggagcagtg ggcattggcg catggtttac
    41401 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
    41461 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
    41521 ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
    41581 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
    41641 gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg ccttcccgcg
    41701 acgcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc tccccctgtt
    41761 tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
    41821 tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg ctggaattac
    41881 cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
    41941 acgaagcggt cagcgtgcgt ttcaaagcgg caggattgct gcatattgtc gggcgcaacg
    42001 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
    42061 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc
    42121 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
    42181 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagttaa tcaggactcg
    42241 ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc cagacgtagc
    42301 tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
    42361 tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
    42421 tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca
    42481 ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
    42541 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
    42601 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
    42661 ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt ggatgcaagt
    42721 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
    42781 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
    42841 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
    42901 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
    42961 atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
    43021 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
    43081 ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg cacccaggcc
    43141 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
    43201 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
    43261 gcgaaagtaa gcgtctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggc
    43321 gtgatctggt tatcggacga taagaataat ctggaagtta acttaaccgc ctggcaacaa
    43381 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
    43441 ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg gccattcatc
    43501 tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt ctactcgccg
    43561 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
    43621 gtcaggaact gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt aaagatttaa
    43681 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
    43741 tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
    43801 gggaataaaa agaaggaata gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
    43861 agcctcttta ctctgatggg atgtaataac cgggccgaag tcgatacgct ttctccagca
    43921 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgctgat
    43981 tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
    44041 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
    44101 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
    44161 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca gttcaactat
    44221 acgctggaac cggcacaatc tagtttacct atgacgccga tgaccctgcg aggcatgtat
    44281 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
    44341 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
    44401 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
    44461 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
    44521 ttggggcagt aaacctgctt aaggcagaaa caaacgcaa
//
LOCUS       DAHBHC010000036        44249 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHBHC010000036 DAHBHC010000000
VERSION     DAHBHC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.036"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..786)
                     /gene="fhuC"
                     /locus_tag="M2A35_002402"
     CDS             complement(<1..786)
                     /gene="fhuC"
                     /locus_tag="M2A35_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCB7198694.1"
                     /translation="MQEYTNHSDTTFALRNISFRVSGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIVQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVS"
     gene            complement(837..3080)
                     /gene="fhuA"
                     /locus_tag="M2A35_002403"
     CDS             complement(837..3080)
                     /gene="fhuA"
                     /locus_tag="M2A35_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCB7198695.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVATCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(3300..5834)
                     /gene="mrcB"
                     /locus_tag="M2A35_002404"
     CDS             complement(3300..5834)
                     /gene="mrcB"
                     /locus_tag="M2A35_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCB7198696.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKTYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQKPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(6030..8459)
                     /gene="hrpB"
                     /locus_tag="M2A35_002405"
     CDS             complement(6030..8459)
                     /gene="hrpB"
                     /locus_tag="M2A35_002405"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCB7198697.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            8533..9063
                     /gene="thpR"
                     /locus_tag="M2A35_002406"
     CDS             8533..9063
                     /gene="thpR"
                     /locus_tag="M2A35_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCB7198698.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            9078..9782
                     /gene="sfsA"
                     /locus_tag="M2A35_002407"
     CDS             9078..9782
                     /gene="sfsA"
                     /locus_tag="M2A35_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCB7198699.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            9960..10415
                     /gene="dksA"
                     /locus_tag="M2A35_002408"
     CDS             9960..10415
                     /gene="dksA"
                     /locus_tag="M2A35_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCB7198700.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            10452..11378
                     /gene="gluQRS"
                     /locus_tag="M2A35_002409"
     CDS             10452..11378
                     /gene="gluQRS"
                     /locus_tag="M2A35_002409"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCB7198701.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            11417..12835
                     /gene="pcnB"
                     /locus_tag="M2A35_002410"
     CDS             11417..12835
                     /gene="pcnB"
                     /locus_tag="M2A35_002410"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCB7198702.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            12832..13311
                     /gene="folK"
                     /locus_tag="M2A35_002411"
     CDS             12832..13311
                     /gene="folK"
                     /locus_tag="M2A35_002411"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCB7198703.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            13681..14265
                     /locus_tag="M2A35_002412"
     CDS             13681..14265
                     /locus_tag="M2A35_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7198704.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            14370..15110
                     /locus_tag="M2A35_002413"
     CDS             14370..15110
                     /locus_tag="M2A35_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCB7198705.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            15145..17746
                     /gene="htrE"
                     /locus_tag="M2A35_002414"
                     /pseudo
     CDS             15145..17746
                     /gene="htrE"
                     /locus_tag="M2A35_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
     gene            17763..18332
                     /locus_tag="M2A35_002415"
     CDS             17763..18332
                     /locus_tag="M2A35_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7198706.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            18347..18949
                     /gene="yadL"
                     /locus_tag="M2A35_002416"
     CDS             18347..18949
                     /gene="yadL"
                     /locus_tag="M2A35_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCB7198707.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            18976..19572
                     /locus_tag="M2A35_002417"
     CDS             18976..19572
                     /locus_tag="M2A35_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7198708.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            19625..20899
                     /locus_tag="M2A35_002418"
     CDS             19625..20899
                     /locus_tag="M2A35_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCB7198709.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            21012..21806
                     /gene="panB"
                     /locus_tag="M2A35_002419"
     CDS             21012..21806
                     /gene="panB"
                     /locus_tag="M2A35_002419"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCB7198710.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            21818..22669
                     /gene="panC"
                     /locus_tag="M2A35_002420"
     CDS             21818..22669
                     /gene="panC"
                     /locus_tag="M2A35_002420"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCB7198711.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNSYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(22743..23645)
                     /gene="rpnC"
                     /locus_tag="M2A35_002421"
     CDS             complement(22743..23645)
                     /gene="rpnC"
                     /locus_tag="M2A35_002421"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCB7198712.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            23919..24299
                     /gene="panD"
                     /locus_tag="M2A35_002422"
     CDS             23919..24299
                     /gene="panD"
                     /locus_tag="M2A35_002422"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCB7198713.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(24303..25532)
                     /locus_tag="M2A35_002423"
     CDS             complement(24303..25532)
                     /locus_tag="M2A35_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCB7198714.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(25596..26036)
                     /locus_tag="M2A35_002424"
     CDS             complement(25596..26036)
                     /locus_tag="M2A35_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCB7198715.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(26141..26911)
                     /locus_tag="M2A35_002425"
     CDS             complement(26141..26911)
                     /locus_tag="M2A35_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7198716.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(26908..27834)
                     /locus_tag="M2A35_002426"
     CDS             complement(26908..27834)
                     /locus_tag="M2A35_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7198717.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            27943..28605
                     /gene="can"
                     /locus_tag="M2A35_002427"
     CDS             27943..28605
                     /gene="can"
                     /locus_tag="M2A35_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCB7198718.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLTQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYCHGISNLKLKHAN
                     HK"
     gene            complement(28646..29182)
                     /gene="hpt"
                     /locus_tag="M2A35_002428"
     CDS             complement(28646..29182)
                     /gene="hpt"
                     /locus_tag="M2A35_002428"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCB7198719.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            29388..31778
                     /gene="gcd"
                     /locus_tag="M2A35_002429"
     CDS             29388..31778
                     /gene="gcd"
                     /locus_tag="M2A35_002429"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCB7198720.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(31825..33375)
                     /gene="cueO"
                     /locus_tag="M2A35_002430"
     CDS             complement(31825..33375)
                     /gene="cueO"
                     /locus_tag="M2A35_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCB7198721.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            33541..33888
                     /locus_tag="M2A35_002431"
     CDS             33541..33888
                     /locus_tag="M2A35_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCB7198722.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            33994..34860
                     /gene="speE"
                     /locus_tag="M2A35_002432"
     CDS             33994..34860
                     /gene="speE"
                     /locus_tag="M2A35_002432"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCB7198723.1"
                     /translation="MAEKKQWNETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            34876..35670
                     /gene="speD"
                     /locus_tag="M2A35_002433"
     CDS             34876..35670
                     /gene="speD"
                     /locus_tag="M2A35_002433"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCB7198724.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(35708..36070)
                     /locus_tag="M2A35_002434"
     CDS             complement(35708..36070)
                     /locus_tag="M2A35_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCB7198725.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(36247..38844)
                     /gene="acnB"
                     /locus_tag="M2A35_002435"
     CDS             complement(36247..38844)
                     /gene="acnB"
                     /locus_tag="M2A35_002435"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCB7198726.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            39199..40956
                     /locus_tag="M2A35_002436"
     CDS             39199..40956
                     /locus_tag="M2A35_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCB7198727.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQRPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(41027..42451)
                     /gene="lpdA"
                     /locus_tag="M2A35_002437"
     CDS             complement(41027..42451)
                     /gene="lpdA"
                     /locus_tag="M2A35_002437"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCB7198728.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(42776..>44249)
                     /gene="aceF"
                     /locus_tag="M2A35_002438"
     CDS             complement(42776..>44249)
                     /gene="aceF"
                     /locus_tag="M2A35_002438"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCB7198729.1"
                     /translation="EGDKASMEVPAPFAGTVKEIKVNVGDKVSTGSLIMVFEVAGEAG
                     AAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDEVEVTEVMVKVGDKIAAEQSLITVE
                     GDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAPAKQEAAAPAPA
                     AKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILR
                     EDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVELGRIQKISGANL
                     SRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVFIMKAVAAALEQM
                     PRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGIIELSRELMTISKK
                     ARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFV
                     PRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM"
BASE COUNT        11033 a        11689 c        10767 g        10760 t
ORIGIN      
        1 actcacaggt gcagcacccg ccggatgcgg caaaataccc atcgggatgc cataaatcat
       61 ttcgagggtt tcgccgcgca taatttccgc aggcgttccc tgaacaatca tttcaccgcc
      121 gcgcagggcg accagataat cacagtagcg tgccgccata ttgatatcgt gcaacacggc
      181 aatgaccgtc aggccacgct cctgacttaa acggtgcacc agcgacagca catcaacctg
      241 gtgggcgata tccagcgccg aggtcggttc gtcgagcaac agacaacggc tatcctgcgc
      301 caccagcatg gcgatccacg cccgctgacg ttcgccgcca gagagactat cgaccagccg
      361 atgcgccagc ggttttaagc caaccagcga gatagcttcc tcgacctttt cgcgatctgc
      421 cgcaccaaag cgccccagcg cgccatgcca cgggtaacga ccaatcgcca ccagttcacg
      481 cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa taagccactt tgcgggcaaa
      541 cgctttgctg ctccagcttt ccagcggctg ggcatcaaga agaatctccc cttctgacgg
      601 aggctgatga cggccaagca ttttgagcag agtggattta ccagaaccgt tgtgaccaat
      661 cagaccggtc actttcccgg caggaaaggt taacgacagc ggatgcaaaa gcgtgcgccc
      721 ggacacacga aaggagatat tacgcagtgc aaaagtggta tcggaatgat tcgtgtattc
      781 ctgcataaca gccaacttgt gaaatgggca cggaaatccg tgccccaaaa gagaaattag
      841 aaacggaagg ttgcggttgc aacgacctga cgttctgcgc cccagaagca gccataagtg
      901 ttaaagcagg tggcgacgta ttcacgatcg aacaggttgt taacatgcag cgccacgttg
      961 gagcccgcca tgccgactcg cgccagatca taacgcacta acgcatccac gaccgtataa
     1021 cttcccactt taaaggagtt agccggatca ccatagctgg agccagtata acgaccaccg
     1081 gtgcccagcg tcagacctga aagcggaccg tcaaagaagg tatagtcagc ccacagcgaa
     1141 gccatgtgtt ttggcacctg tgcaggcgta ttgcctttat aggtagtatc ggtggtgtat
     1201 tccgcatcgg tgtaagtata agaaccgact acgttaacac tcgccgacag cgccgctttc
     1261 gcttcgattt ctacgccacg tgcgcggatc tcgccacctt caaccgagaa gaaggaaccc
     1321 tcagggtccg ccatcaggtt gttggtttta gtgagattat acacggcacc agtaactaca
     1381 atcggacgat cttccggtac atatttcacg ccgacttcat actgcttacc tttagacggt
     1441 gcgaaaatat taccatcctt cccaacttgc gaagaaggtt caaacgattc gctatagctg
     1501 aagtaaggtg ttacaccatt atcaaacagg tagttaacac caccacgcca ggtaaactgt
     1561 ttgtcatcac gtttatcggt cgtcccagca acgcggttaa gagattcttg atctgcccag
     1621 tcataacgac cgcccagggt gaccagcact ttatcccact gcgcctgatc ctgaacataa
     1681 acgcccgttt gtttctgctt attcagaatg cggtaagggc cggagtttgc cggatctttg
     1741 gcattgaagt cgaaatcggt attcaccgga ttgtacagat tgagcagtgg cacagagtcg
     1801 tcgtaaccaa accaggcgtt gatgtcatta cgcatacgca taaagtcgac accggtcagc
     1861 agggtgtggt cgatatcgcc agtggcaaac ttgctctgca actgggtatc aacggagaag
     1921 ttttgcagct tctcatcatc aacgacgtat ttacgtgcca gataatggcc tttatccgct
     1981 ggcgctaatg ccgcacactg tttgctgtaa gcattcgccg gatcggagca gacgccgtaa
     2041 ccataaacgc tgttttgcga ggttttgttt tcagcaaagc gcaggttctg acgcacagta
     2101 aaggtgtcgt taaattcgtg atcgaagctg tagccgacca tcttctcatt acgagaatag
     2161 gtgttgttct tcgccccttc attaaagtct gtcggcagac gcttaccgtt cggcagcggc
     2221 tcaacggttc cctctttcgg caaccagccg taataaccgg tttccggctc gttctggaag
     2281 taagaaagga aggtgaaatt ggttttatca tccggacgcc aggtgaacgc cggtgcaata
     2341 gcataacgct gctcttctga ccctttctgc tgggcattgg cagaacgcgc aagaccggtc
     2401 aggcgataag agtaaacacc gtcatcatcc aacgaatcgc taaagtcaaa accagtctgg
     2461 aacaggctgt cagtaccggc tttaaactga acttctttca gcggttcggt ggtcggacgc
     2521 ttgctgacca tattcaacag gccgccagga ctgcttttac cgtaaagcac ggaaaccggg
     2581 ccacgcataa tttcagcgcg ttccagcata tacgggtcaa tgaccgcatc gttatagaag
     2641 ttgccctgca acttcaggcc attcagatag ttattctggc tttggccttc tgccgcaaag
     2701 ccgcgaatga tcaggtggtc ataggtgttg gatgcgccac gcgtaccaac agagacaccc
     2761 ggcgtgtagc taagcgcttc ttttaccgac ttcggctgat gcagcgccat ctcttcggcg
     2821 gtcacaacag aaatagactg tggcactttt tgaatcggcg tatcggtttt agtgccggta
     2881 gcagactgtc gcgccgcaat agttgcagca ggcccccatg cgctttcttg cggcgcaggt
     2941 gcagcggtaa cggtgatagt gtcttctttc ggttcaaccg ctgcctgtgc ataaacagac
     3001 atgccgctta ccgctgtggc tactacaact gcgattttac gcagtgagtg ttttggctga
     3061 gcagttttgg aacgcgccat tggtatatct ctgatgtaaa gtgaatgata acgtaaacga
     3121 gaatgattat tataaagata agataatatt cgaaacgctg gcgggttcgc aagcggtacg
     3181 caatctgagc gttcctgaag ggttaataaa caaccagatg aaaagaaagg gttaatatct
     3241 tagatgggat gttattttac cggatggcaa ctcgccatcc ggtatttcac gcttagatgt
     3301 taattactac caaacatatc cttgatccaa ccggctacac cgtcgctgtc tttctgctct
     3361 tgctgagcag gttgctgttg cggctgttgc tgcggctgag aagactgatc aaacggattg
     3421 cctgacggct tctgctgcat ctcgctctgc tggcacagcg attgcggatc gctggtccag
     3481 accggcaaga cacgcatgcc accgctgcaa acaaagttgc cgtcgtagtc cacgcccata
     3541 tctgcaatat cttctggcgg aacaagattc agcggcgttg gcgtctggtt agccagataa
     3601 cgctgataaa tcgacattgc cccgctggct ccatacagtt tggtcggctg gttgttatca
     3661 cggcctaccc aggtgatggt caccgtgctg ccgtcaatgc ctgcaaacca ggtatctacg
     3721 ttattgttgg tagtccctgt tttccctgcc agatgcaggt tcgggtattt cgccccaagc
     3781 tgacgacccg taccgcgttg taccacctgc tgcatggtcc atagtgtcag atacgccgcc
     3841 tgcgccggaa cagcgcgttc cgcctgcggg aagctctgat acagcacctt gccatcttcc
     3901 gcaattaccg aacgcagtgc agaaagcggt gcacggttac cgccgctggc gatggtctgg
     3961 aacgcctgcg ccacttcgat tggcgttaag ttcaacgccc ccagcagcat cgccggaacc
     4021 ggatgcaact gatctttcgg tacgcccagt ttgatccagg tctccgtaac cgcaggcagc
     4081 cccagcgcca tccccagatt taccgtcggc acgttcatcg aacgggtcaa cgcatccacc
     4141 agcatcactc tgccgctttc gctataacga cggtcatcat tctgcggtga ccagacctgg
     4201 ccattcggct gacgcagcgc gattggcgca tccgcaatcc acgtattcag acgataggtt
     4261 ttcggctggc ttaaggcagt cagataagtc gctggttttg caagggaacc aatcgaacga
     4321 cgcgcctgca tcgcacggtt gtagcccgca aactgcggct cagaaccacc gaccatcgca
     4381 cgaacttcac cactaaagcg gtcgacgacc acaatcgcag tttcaagatc gctcaactta
     4441 cgctgtttct tcagtgccgg aatgccttcc acggcggctt tttctgccgc gtcctgggcc
     4501 accgagtcaa aggtagtgaa gatcttcacg ccggagagat cttttacctt atcgcctaat
     4561 ttcgcctgca gctcctgacg caccagttgc ataaaggctg gctgtggaga gatcacccca
     4621 ccgcgtggct gaacacccag cggacgtgca ctcaacatgt catagagttc ttgatcaata
     4681 atctgttgct gttgcagcag acgcagcacc agattacgtc gctccagcgc cagttttggg
     4741 ttacgccacg ggttgtagat ggacgcccct ttcaccatac cgactaacag cgcctgctgg
     4801 tcgaggctta gctcttctac cgggcgacca aagtaataca agcttgccag cgggaagccg
     4861 cggatttcgt tgtcgccgct ctgaccgaga tacacctcgt tcatatacag ctcaagaata
     4921 cggtctttgc tgtaacgcgc gtccatgatc agcgccatgt aagcttcgtt cgctttacgc
     4981 cagtaagaac gctcgctgga gaggaacagg tttttcacca gctgttgcgt cagcgtactc
     5041 gcaccctgta ccgtgcgtcc ggcggtcagg tttgccagca ccgcacgtcc gattgagtag
     5101 agactgattc catcatgctc gtaaaaatga cggtcttctg tcgccagcaa agtatccacc
     5161 agcaaatccg ggaaaccact gcgcggcaca aacagacgct gctcaccgtt tggcgaagag
     5221 atcatggtga tcagacgcgg atcaagacgg aagaaaccga actgacggtt gttctccata
     5281 ttgacgatcg tcgccagatg atcgccatca aaggtcagac gcgcgcgcac ctgtccttct
     5341 ttactgtccg ggaaatcaaa cggacggcga atcatctcaa tgctgttggc ctgcacggta
     5401 aattcgccag gacgggtcat tttcgacacc tgacgatact gggtcgcctc cagcagcttc
     5461 accatctcgt tcttgctgat ggtcatgtct ggctcaagat tgaccattcg gccataaact
     5521 gccgcaggca gttgccagac cttgccatca atacggctac gaattttttg atcgagataa
     5581 acgccgtaaa tggcgatcag cacggcaaaa acgatagcca gttttagcag tagccatagc
     5641 cagccgcgtt tgccacgagg cttacgccct ttgcctttgc ccttaccttt gcgcggcatc
     5701 ggttcttcat cctcatagtc atcataatcg tcgtaatcat cgtcatcttc gtaacgacga
     5761 cggcttacct tttgtttgac cggacgcgtc ggtttccctt tgcgtccaat tggctcgcgg
     5821 tcattcccgg ccatgctttt tctccgcaat attcaggcgc aaaagcccga ttttctgttc
     5881 ttctgttaca agacagaaga agatatctct caaaatttgt gctcgatcgg tcccctctcc
     5941 cttgagggag agggttaggg tgagggtgaa cgtgcgcgct gatgccctca ccccggccct
     6001 ctcccacagg gagagggaga aaaccaacct tacgaatact ttttcgtccg tcgcgtcggt
     6061 gcagtatttg ccgggtcgtc cggccagaca tgtttgggat aacgcccttt catctctttt
     6121 tgcacctcac ggtacgctcc tttccagaag gcgctcaaat ctcgtgtgat ttgtaatggc
     6181 ctttgggcag gtgaaagcaa ctccagcacc agcggcacgc gcccctgggc gatcgtcgga
     6241 ttggtggcct cgccaaacat ctcttgcatt ctcaccgcca gcgcgggcgg gttatcttca
     6301 tgataacgaa tggcgatccg gcttcccgtc ggcacagtgt aatgcgcagg caattcacta
     6361 tccagacgtt gctgcattcc ccaatcaagt aatccgcgta gtgcctgata aatgtcgagt
     6421 gatttcaggc cgcgtagtga atgtacgcca gtcatatgtg gcagcagcca cgtttccagc
     6481 gtcgccaata aactttcatc atcaaccgct ggccagtcat attccggcaa ccactttgcg
     6541 gcgcataaca aacgcaagcg tagctgttcc gcttccgccg tccagttgag cacgcttaaa
     6601 cctttttcac gaatgccgtt aagcatcgcc tgatgcaact cgtcttctga cggtttcgcc
     6661 agtggctgca ctttcaccgt caactgaccg atttgcagcc gacgccaggc tttcagcgta
     6721 ccttgcgcgt catcccattc cacggtgtca gattgctgca ccagctgcgg acagcgttgt
     6781 actaattcat caatatcgac cggtaacgcc agtaaaatcc gcgcatccgg cgaggcgctg
     6841 ccctgcaata ataacggtgc gatcaaccat tcgtggcggc ttagcgcgtc gttggcatcg
     6901 agcatcgctc ccatgccgtt tgccagttga tagcgtccat cttgcccacg gcgacgagca
     6961 atgcgatcgg caaacgcccc ggcaagtagc ggcgcgataa gcgaactgtc tgcctcaccg
     7021 ccacgtacgt ttaagcgttt taacagttgc tgactacgtt gctgccaggc tggttgattg
     7081 cgcgaaaacg ccacgcccag gtcactattg cccatccgtg gcggctcttc gagaatggcg
     7141 gcaatttttg ccgcggtagc agcttcgtcg tcgttcttcg cgctaaccag cattgccgct
     7201 aaacgcggat cgttacccag cgctgccatt ttttgcccct gcgcactaag ccgttcaccc
     7261 tccagcgccc ccagcatttg taacaggcgt ttcgcggcca gtagattcac cgttggcggt
     7321 tgatccagcc agctcatctg cgccggatcg ctgcatcccc attgcagtaa ttccatcagc
     7381 aaaccggaaa gatcgctttg taagatctcc ggttcacttt gcgccgcggc gcgttctgct
     7441 tgttctttgg cgattaaatg caggctgata cccggctcca gacgcccggc gcgcccggca
     7501 cgctgcgtca tggatgcctg gctaacgcgt tgagtaatca gtcgcgtaag ccccgtgcgc
     7561 ggatcaaaac gcgccacacg ctcctgggca caatccacca ccagacgaat accttcaatg
     7621 gttaaactgg tttcagcaat attggtcgcc agcaccactt tgcgcatccc ttgcggtgcc
     7681 gggaggatcg cttttcgctg atcgttcagc gacaacgcgc catacagcgg gcagagcaac
     7741 acatcactgc cgatgcgcga agccagttgt tcctgcacac gctgaatttc tccgacgcca
     7801 ggtaaaaata acagtaatga tccgctttcc tgacgcagca tttcggcggt ggctaccgca
     7861 acggcttcgt caaaacgctg atgcgcgggc agcggtaaat aatggcgttc aaccggaaac
     7921 gagcgccctt ctgagatgac gacgggcgct tctggcagca tttgctgcaa gcggtcgtta
     7981 tccagcgtag ccgacataat cagcagttta aggtcatcac gcagaccttg ttgcacatcg
     8041 agtaacagcg ccaacgccaa atccgcctgc aagctgcgct catgaaattc atcgaggatc
     8101 accaatccaa caccgctcag ttccgggtca cgctggatca tgcgcgtcag cacgccttcg
     8161 gtaaccactt ccaggcgggt attcggcccg acgcagtttt gcgcacgcat ccggtagcca
     8221 acggtatcgc ctggcttttc gttaagcagc tccgccagtc gttgcgcgac gttacgcgcc
     8281 gccagacgac gcggctccag caggataatt ttcccgttaa tgccgggatg cgccagcagt
     8341 tgcagcggca gccaggttga tttcccggcc ccggtcggcg cacttaataa tacctgcggc
     8401 gcacaatcga gagcggtaag taattcagat aagacggcag caacgggcaa cgacgacaca
     8461 aaacgctcca gagggttaac attcttcgcg ccacattgta gcatcgcggt aattcataac
     8521 cgagtgcctc acatgtctga accgcaacgt ctgttctttg ctatcgactt acctgcagaa
     8581 atccgcgaac agattatcca ttggcgcgcc acacacttcc cacctgaggc gggacgtccg
     8641 gtcgccgccg ataatttgca tctgactctg gcatttttag gcgaagtgag cgccgagaaa
     8701 gagaaggcgc tttctctttt agccggacgg attcgtcaac ctggtttcac actcacgctt
     8761 gatgacgccg gacaatggct gcgttcgcgt gtggtgtggt tagggatgcg tcagcctcca
     8821 cgcggcttaa tccagctggc gaatatgctc cgttcacagg ctgcccgcag cggttgtttt
     8881 caaagcaatc gtccgtttca tccacatatt accttattgc gcgacgccag cgaggcggtg
     8941 acaatcccgc cgccaggttt taactggtct tatgcggtga cggagttcac cctttacgcc
     9001 tcctcgtttg cccgtggacg cacacgctac acgccgctaa aacgctgggc gctaacgcaa
     9061 taacaaggat tgtcgcaatg gaattttctc cccctctaca gcgcgcgacg ctaattcagc
     9121 gttacaaacg ttttttagcc gatgtgatca cacccgatgg tcgcgaatta acgctacact
     9181 gcccgaatac gggtgcgatg accggttgtg caacgcctgg cgataccgtc tggtattcga
     9241 cttcagacaa caccaaacgg aaatacccac acacctggga actaactcaa agccagagcg
     9301 gcgcatttat ttgcgtcaat acgctttggg ctaacaggtt gacgaaagag gctatcctta
     9361 atgaatcaat ttcagaactg tcaggctata gctcgctgaa aagcgaagta aaatacggcg
     9421 cagaacgcag ccgtattgac tttatgttgc aggcggattc gcgtccagac tgctatattg
     9481 aagtgaaatc ggttacgtta gcggagaacg aacagggata ttttcccgat gcggtcactg
     9541 aacgaggtca gaaacacctt cgggagttga tgagcgtagc ggctgaaggc cagcgtgcgg
     9601 ttatcttttt cgccgtgctg cattcagcca ttacacggtt ttcacccgcg cgccacatcg
     9661 atgagaaata cgcgcaacta ttgtcagaag ctcaacagag gggggtagaa attctggctt
     9721 acaaagcgga aatttctgct gaaggcatgg ctcttaaaaa atcactgccg gttacattgt
     9781 agtaaagtaa gtaactggtt aatttacatt ctggtcgcgt gcgcaaatac gcttttcctc
     9841 acacagttgt caagtgttac gtttagataa ttgctatccg gaaaagcatc tgctatttat
     9901 agcggcctca tttttccccc gaacatgggg atcgatagtg cgtgttaagg agaagcaaca
     9961 tgcaagaagg gcaaaaccgt aaaacatcgt ccctgagtat tctcgccatc gctggggtgg
    10021 aaccatatca ggagaagccg ggcgaagagt atatgaatga agcccagctg gcgcacttcc
    10081 gtcgtattct ggaagcatgg cgtaatcaac tcagggatga agtcgatcgc accgttacac
    10141 atatgcagga tgaagcagcc aacttcccgg acccggtaga ccgtgcagcc caggaagaag
    10201 agttcagcct cgaactgcgt aaccgcgatc gcgagcgtaa gctgatcaaa aagatcgaga
    10261 agacgctgaa aaaagtggaa gacgaagatt tcggctactg cgaatcctgc ggtgttgaaa
    10321 ttggtattcg ccgtctggaa gcgcgcccga cagccgatct gtgcatcgac tgcaaaacgc
    10381 tggctgaaat tcgcgaaaaa cagatggctg gctaattaca gccgttccat cacgtttacc
    10441 acaggcggga aatgctcccg ccttattttt tgttcaaaga gatgacagac acacagtata
    10501 ttggccgctt cgccccctct ccttccggcg agcttcattt tggctctctg atcgccgcgc
    10561 tcggcagcta tttgcaggct cgcgcccggc aaggtcgctg gctggtacgc atagaagata
    10621 tcgacccgcc tcgtgaagtt cccggtgccg cagaaactat cctgcgccag ctggaacatt
    10681 acggtctgca ctgggacggc gatgttctct ggcaatcgca acgtcacgac gcctatcgtg
    10741 aagcactcgc ctggttacat gaacaaggac taagttatta ctgcacctgt acgcgtgcgc
    10801 gtattcaaag cattggcggt atttacgacg gtcattgccg ggtgttgcat catggaccag
    10861 acaacgccgc agtgcgtatc cgccagcagc atccggtcac gcaatttact gaccagctgc
    10921 gcggcattat tcacgccgac gaaaaactgg cacgggaaga ttttatcatt catcgccgtg
    10981 atgggttgtt cgcctacaac ctggctgttg tggttgatga tcatttccag ggcgttacag
    11041 aaatagtgcg tggggctgat ctgattgaac caacagtaag gcaaatctcg ctgtaccagc
    11101 tttttggctg gaaagtgcca gattacattc atctgccgct ggcgcttaat ccacaaggcg
    11161 ctaaactttc caagcagaat catgcgcctg cgttgccgaa aggcgatcca cgcccggtac
    11221 taatcgcggc acttcaattt ctggggcagc aggcagaagc acactggcag gatttcagcg
    11281 tcgagcaaat ccttcagtca gccgtcaaaa actggcggct taccgccgtg cccgagtcgg
    11341 caattgtaaa ttcaacattc tcaaatgcgt catgctgagc tatgattagc cgctattttt
    11401 ttgtcctgaa tgatgtttga cactaccgag gtgtactatt tttacccgag tcgctaattt
    11461 ttgccgcaag gtgctaagcc gcgaggaaag cgaggctgaa caggcagtcg cccgtccaca
    11521 ggtgacggtg atcccgcgtg agcagcatgc tatttcccgc aaagatatca gtgaaaatgc
    11581 cctgaaggta atgtacaggc tcaacaaagc gggatacgaa gcctggctgg ttggcggcgg
    11641 cgtgcgcgac ctgttacttg gcaaaaagcc gaaagacttt gacgtaacca ctaacgccac
    11701 gcctgagcag gtgcgcaaac tgttccgtaa ctgccgcctg gtgggtcgcc gtttccgtct
    11761 ggctcatgtg atgtttggcc cggagattat cgaagttgcg accttccgtg gacaccacga
    11821 aggtaacgtc agcgaccgca cgacctccca acgcgggcaa aacggcatgt tgctgcgcga
    11881 caacattttc ggctccatcg aagaagacgc ccagcgccgc gatttcacta tcaacagcct
    11941 gtattacagc gtagcggatt ttaccgtccg tgattacgtt ggcggtatga aggatctgaa
    12001 ggacggcgtt atccgtctga ttggtaaccc ggaaacgcgc taccgtgaag atccggtacg
    12061 tatgctgcgc gcggtacgtt ttgccgccaa attgggtatg cgcatcagcc cggaaaccgc
    12121 agaaccgatc cctcgcctcg ctaccctgct gaacgatatc ccaccggcac gcctgtttga
    12181 agaatcgctt aaactgctac aagcgggcta cggttacgaa acctataagc tgctgtgtga
    12241 atatcatctg ttccagccgc tgttcccgac cattacccgc tacttcacgg aaaatggcga
    12301 cagcccgatg gagcggatca ttgaacaggt gctgaagaat accgatacgc gtatccataa
    12361 cgatatgcgc gtgaacccgg cgttcctgtt tgccgccatg ttctggtacc cactgctgga
    12421 gacggcacag aagatcgccc aggaaagcgg cctgacctat cacgacgctt tcgcgctggc
    12481 gatgaacgac gtgctggacg aagcctgccg ttcactggca atcccgaaac gtctgacgac
    12541 gttaacccgc gatatctggc agttgcagtt gcgtatgtcc cgacgtcagg gtaaacgtgc
    12601 atggaaactg ctggagcatc ctaagttccg tgcggcttat gacctgttgg ccttgcgagc
    12661 tgaagttgaa cgtaacgctg aactgcagcg tctggtgaaa tggtggggtg agttccaggt
    12721 ttccgcgcca ccagatcaaa aagggatgct taacgagttg gatgaagaac cgtcaccgcg
    12781 tcgtcgtact cgtcgtccac gcaaacgcgc accgcgtcgt gagggtaccg catgacagtg
    12841 gcgtatattg ccataggcag caatctggcc tctccgctgg agcaggtcaa tgctgccctg
    12901 aaagcattag gcgatatccc tgaaagccac attcttgccg tttcttcgtt ttaccgcacc
    12961 ccaccgctgg ggccgcaaga tcaacccgat tacctcaacg ccgccgtggc gcttgaaact
    13021 tcgctcgccc cggaagagtt actcaaccat acacagcgca tagaactgca acagggtcgt
    13081 gttcgtaaag ctgaacgctg gggaccacgc acgctggatc tcgatatcat gctgtttggt
    13141 aatgaagtga taaataccga acgcctgacc gttccgcact acgatatgaa gaatcgtgga
    13201 tttatgctgt ggccgctgtt tgagattgca ccggagctgg cgtttcctga tggggagacg
    13261 ttgcgtgaag tcttacatac aagagcattt gacaaattaa gcaaatggta atttatttac
    13321 attacgaata taaatatttt ctgaaaatca tacatcctct ctttccttaa taaaaatttt
    13381 agtttaaaca tttttgcaga aacataatat aaattcccgc agataaatat caatcccatc
    13441 gccataccct aaccaattgc acttgtgaat ttatttttgc ttaattttga attaagaaaa
    13501 tttaatttgt tcaaaagccc ttaaagtgca caataggcgt cctaatgaac aatggttgta
    13561 aattttggta acaaatatca ttgctcatta atatctagca tctgcttata acatttgtgt
    13621 catgaatagt cattcttcaa accagtagtc tgactaatgg aattttattc aaggattttt
    13681 atgtctaaaa aattaggttt tgccctgtct ggtcttatgc tggcaatggt tgcaggtact
    13741 gcttctgctg atatggatgg tggtcagtta aatatcagtg gtctggttgt tgataacacc
    13801 tgtgaaaccc gcgttgacgg cggtaataaa gatggtctga tcctgctgca aactgcaact
    13861 gttggtgaaa tcgatgctgg cgtgctgaat gacaccgttg gcgctaaagc caaacctttc
    13921 agcatcaccg ttgactgcag caaagcaaat ccgaatccag gtagtacagc taaaatgacc
    13981 ttcggttctg ttttctttgg taatagcaaa ggaacgctga acaacgacat gtctattaac
    14041 aacccgtctg acggcgtgaa tatcgctctg cacaatattg atggttccac tatcaaacag
    14101 gttcaaatta acaaccctgg cgatgtgtat actaaagccc tggatgcaac gacaaaatct
    14161 gctgtttatg attttaaagc gtcttacgtt cgtgccgtcg cagaccaaac agcaactgct
    14221 ggttatgtaa aaactaacac tgcatacacc attacttatc agtaagtatt caccaattaa
    14281 attgattgaa tgaatataca gggaatagtg ttaataattt ctatttttat attattccct
    14341 gttttaatta actctgtcag ggatggttta tgttttttaa caccaaacat acaacagctt
    14401 tatgcttcgt aacctgtatg gcttttagtt catcgtctat tgcggacatt gtcatttcgg
    14461 gtactcgcgt aatatataaa agcgatcaaa aaagtgtcaa cgtacgtctg gaaaataaag
    14521 ggaataaccc gttgcttgtc cagagttggt tagatactgg cgatgacaac gctgagcctg
    14581 gcagtattac agtccctttt actgccacgc cgccagtatc gcgtattgat gccaaacgtg
    14641 ggcaaacaat caaattaatg tacacagcca gcacctcact gcctaaagac agagagagcg
    14701 tgttctggtt taacgtactg gaagttccac caaaaccaga tgcagaaaag gtcgcgaatc
    14761 aaagtctgct gcaactggca tttcgcacac gtataaaact tttctatcgc ccggatggat
    14821 tgaagggaaa tccctctgaa gccccgttag ccctgaagtg gttctggtca ggttcagaag
    14881 gtaaggcgtc attgcgagtg accaatccaa ccccttacta cgtctctttt agcagtggtg
    14941 atttagaagc tagcggtaaa cgctatccga ttgatgtgaa aatgattgca ccatttagtg
    15001 atgaggtcat gaaagtcact ggccttaatg gcaaagcaag ttctgcaaaa gtacattttt
    15061 acgccattaa tgattttggt ggcgccattg aaggtaatgc cagtctgtaa tcaggcaagg
    15121 atataattcc gcaggaagca taacgtgact ataaaaccaa ctaaaaatta tcataatcat
    15181 ctgacccgta tcgctacgtt ttgcgcgctg ctgtattgca atagtgcttt ctgtgctgaa
    15241 cctgttgaat atgaccatac cttcctgatg gggcagaatg cctcaaatat tgatctcagc
    15301 cggtatagtg aaggtaaccc tgctataccg ggtatgtacg acgtcagtgt ttatgtcaac
    15361 gatcaaccta ttattaacca aagcattaca tttattgaaa ttgaaggaaa aaaaaacgca
    15421 caagcctgta tcacacttaa gaatttattg cagtttcata taaattctcc cgatataaat
    15481 aacgaaaaag ccgttctgct tgccagggat gaaacactcg gcaattgcct taatttgacg
    15541 gaaattatcc ctcaggcttc tgttcgttat gacgttaacg agcaacgtct ggatatagac
    15601 gttcctcagg catgggtaat gaaaaattac caaaactatg ttgatccatc gttatgggaa
    15661 aatggcatta atgcggccat gttgtcatac aacctcaacg gatatcatag tgaaactcct
    15721 ggtcgaagaa atgacagcat ttatgctgct tttaacggtg ggatgaattt aggtgcatgg
    15781 cgactgcgtg cctcaggcaa ctacaactgg atgaccgatt ctggcagtaa ttatgatttt
    15841 aagaatcggt atatacagcg tgatatcgcc tcgctgcgtt ctcaactcat tcttggtgag
    15901 tcttatacaa cgggcgaaac ctttgattct gtcagtatcc gcggcattcg tttatacagc
    15961 gacagccgca tgttgcctcc gacattagcg agttttgcgc ctatcattca tggcgttgct
    16021 aataccaacg ccaaagtcac tattacgcaa ggtggctata agatttatga aacgactgtg
    16081 ccgccaggcg ctttcgtcat tgatgatctg agtccgtcag gttacggcag cgatcttatt
    16141 gttactgtcg aagaatccga tggctcaaag cggacattct cgcaaccttt ttcatcggtc
    16201 gttcaaatgt tacgccctgg cgttggacgt tgggatatta gcggcggcca ggtcttaaaa
    16261 gatgatattc aggatgagcc taatttattt caggccagct actactacgg tctgaataac
    16321 tatctaacgg gttatactgg tattcagatt accgataata actataccgc tggtttgtta
    16381 ggtcttggtc tgaatacttc agtcggtgca ttttctttcg atgtgactca ctccaatgtt
    16441 cgtataccgg atgataaaac atatcaggga caaagttatc gtgtttcctg gaacaagtta
    16501 ttcgaagaaa caagtacttc actgaatatc gcggcctatc gctattcgac acagaattac
    16561 cttggtctta atgatgcact aactctaatt gatgaagtga aacatcccga acaagatctt
    16621 gaaccgaaat ccatgcgtaa ttactcgcgc atgaaaaatc aggttacggt cagtattaac
    16681 caaccgctaa aatttgagaa aaaagattac ggttcatttt atctttccgg aagttggtcc
    16741 gattactggg cttccggaca aaatcgtagc aattactcta ttggctacag taacagtgca
    16801 tcctggggca gctacagtgt cagtgcccag cgttcatgga atgaagacgg cgacactgac
    16861 gatagcgttt atctcagttt caccattcct attgaaaaat tacttggcac tgaacaacgg
    16921 acttcaggtt tccagagtat tgatactcaa atgagcagtg actttaaggg taataaccaa
    16981 ctcaacgtca gtagcagtgg ctatagtgat aacgctcgcg tcagttatag cgtaaatact
    17041 ggctatacga tgaataaagc cagcaaagat ttgagttatg ttgggggtta tgccagctat
    17101 gaatcaccgt ggggaagcct ggcaggttca gtttctgcaa atagcgataa cagtcgtcaa
    17161 gtgtctctca gcaccgacgg tggttttgta ttgcatagcg gcggcctgac cttcagtaat
    17221 gacagtttta gcgactccga tacactggcg gtagttcagg ctccaggtgc tcaaggagcg
    17281 cgaataaatt atggcaacag tactatcgat cgatggggtt atggtgtcac cagcgctctt
    17341 tctccttatc atgaaaaccg tatcgcgctg gatatcaacg atcttgagaa cgatgttgaa
    17401 ttaaaaagta ccagtgcagt agctgtaccg cgtcagggtt cagtcgtctt tgctgatttt
    17461 gaaaccgtgc aagggcaatc agccattatg aacatcacac gaagtgatgg taaaaatatt
    17521 ccatttgctg cagatattta tgatgagcaa ggcaatgtca ttggtaatgt tggacagggt
    17581 ggacaagcat ttgttcgtgg tattgagcag cagggaaata tcagcattaa atggctcgaa
    17641 gaaagtaaac ccgtaagttg tcttgcgcat tatcaacaaa gttcagaagc agaaaaaaat
    17701 agcgcaatct attattctga atggaattcg gtgtcagatt cagtaactac aagggacgtc
    17761 aaatgataaa aataacgcca cataaaatag cgatactgat gggaatatta ttatcaccat
    17821 cagtatttgc aacggatatt aatgtagagt ttacagccac tgtcaaagcg acaacctgta
    17881 acatcacact tactggtaat aacgtcacga atgatggcaa taataactac acattgagaa
    17941 tccctaagat gggtctggat aagatcgcga ataaaacgac agaatctcag gctgatttta
    18001 aactggttgc tagtgggtgc agcagtggca tcagttggat tgataccact ctgaccggaa
    18061 atgcatcatc aagctcacct aagcttatta taccgcagtc tggtgattca tcttcgacga
    18121 caagtaatat cggtatgggt ttcaaaaaac ggactactga tgatgccact ttccttaaac
    18181 ctaacagtgc ggaaaagata cgctggagca cagacgagat gcagcccgat aagggtcttg
    18241 aaatgaccgt tgcgctacgt gaaacagatg cagggcaagg cgtaccgggg aattttcgtg
    18301 cactggcaac gtttaatttc atctatcaat aagaaggata attaagatga catttaaaaa
    18361 tttacgttat ggattatcca gcagtgtcgt tttggctgcc tcattgttca gcgtaccctc
    18421 ttatgcggca acagacagca ttgggctgac cgtgattact accgtagaaa taggtacttg
    18481 tactgctaca ttagtaaatg actctgatca ggacatttct gttgttgact ttggcgatgt
    18541 atatatttct gaaatcaatg ccaagaccaa agttaaaaca ttcaaactca aattcaaaga
    18601 ctgtgcgggt atccccaata aaaaagcaca aataaaatta accaagcgag ccacatgcga
    18661 aggtactgct aatgacggtg cggggttcgc aaatggatcc acagccgcag ataaagcaag
    18721 tgccgtcgcc gttgaagtct ggagcaccga aactccggca acagggagcg caacacaatt
    18781 tagttgtgtg acaccagcat ctcaagaggt cactatctcc aatgcagcca acgcggttgt
    18841 ttattatccg atgagtgctc gactggtcgt cgaaaaaaat aaaaccgtaa gcaatgtcac
    18901 tgcgggtaaa ttttctgcac ctgccacatt tacagtaacc tataactaac agactgacag
    18961 atccaggaac tatatatgca tcccactcaa cgtaagctga tgaagagaat aattctgttt
    19021 ctgtcactac agtttcccat tacctatcca gccatcgctg ggcaggatat tgaccttgtt
    19081 gccaatgtaa aaaacagcac ttgcaaaagc ggaatcagta accagggtaa tattgatctt
    19141 ggcgtcgttg gggtgggata tttttcagat aatgttactc ctgaaagcta tcaaccaggt
    19201 ggaaaagagt tcactgtcac agtatcagac tgtgcattac agggaactgg cgatgtgctt
    19261 aatcagttac atattgattt tagagccctt agcggtgtca tggctgcagg ttcaaggcaa
    19321 atatttgcca atgaagtcgc aaccggggct aaaaatgtcg gtgtcgttct cttttccatt
    19381 caggacccga ccaatatatt caatgttatt agttcagcag gaaattctcg ttccgtgtat
    19441 ccagtaatga cttctgccct gcataattct tcatggaaat tttatgccag aatgcaaaaa
    19501 atcgatcccg cgctggatgt catatctggc caggtaatga gtcatatatt agttgatgtg
    19561 tactacgaat aatagatcaa aacaaataac tgcgactgtt acagcagtag atggtggagt
    19621 gataatgaag aaattcttca gacattttct ttttttaatt ctgtgcttga gttgttatac
    19681 cgcgagtgct ggcactgatg ataacgtagg atatattgtt gggaatagtt acggcgttgg
    19741 tccgtcagat caaaaatgga gagaaacggg gccgaatggc gatgctactg tcatattcag
    19801 atatgcaaca agtacaaata atttagtttt ctacaaaccg acgcagcttg gacctacagg
    19861 tgtaaaatta cagtggagtc agttagatac cgcttctggt ggtggttttc tttattgcaa
    19921 cagaagtgat agcacaagtg gtagcgcaat gcgtattgaa aatgcaatgg ttgactcagg
    19981 taaaatgtat ggctcccata aattatttaa tacatcagtt cctggtttgt attacacatt
    20041 attaatttca aacatgtggt cagcttacgg taccgtaact aacgttagtt cacctgggat
    20101 atacattggt gactctgcag aacaatattt ttcgtggtat aatccaagcg aagacgtgtt
    20161 atattggagt tgcaataatg cgaatagcac ccgtaaatac tgggctgtag gtggtattta
    20221 tcagaccctt acaattgaat tctatacaga tacaaacttt gatccaacag taactcaaca
    20281 gatcaaatta tccagctcat caaattatct gtattcattt aaagcctatg gcgcaggcca
    20341 aggtataaat gagcatagtt attttatcaa aatcgatttc gatctgctca atgtcaagtt
    20401 gactaatccc acttgtttta ccgctatgct tagtggaact tctgtgactg gttctacggt
    20461 aaaaatgggt gaatatagtg cagaacaaat cagaaacggt gccacaccgg ttccttttga
    20521 tatttcactt caaaactgcg ttcgtgtgac taatattgag acaaaattag tttcaacaaa
    20581 ggttggtact gaaaacgggc aactccttgg taatactctc acaggtaatg acgcagcaaa
    20641 aggagtcggt gtactcatag aaggtttagc aactagtaaa aatcctctaa tgacattgaa
    20701 acctaatgat tcaaattctg tttataaaga ttacgacccg agaggcaaag acgatacaac
    20761 aggaggagtt tacccggatc aagatacagg tataacatac cctctccatt tccaggccac
    20821 gctacaacag gatggaacta taccaataga agctggtgag tttaaagcca ccagtacttt
    20881 ccaggtaacc tacccttaat aagtccaccg caccgccatc atctggcggt gcggtaattg
    20941 ataaatcatt gcccccacca atgtgactga taaaatgcca ggcaacacga catcatttat
    21001 caggacacgt tatgaaaccg accaccattg cttcacttca gaaatgtaag caggataaaa
    21061 agcgtttcgc gaccatcacc gcttacgact acagcttcgc gaaactattt gctgatgaag
    21121 gacttaacgt catgctggta ggcgattcgc tgggcatgac ggttcagggc cacgactcca
    21181 cccttcccgt taccgtcgag gatatcgcct atcataccgc cgccgtacgt cgcggcgcgc
    21241 caaactgcct gctgttggct gacctgccgt ttatggcgta tgccacgccg gaacaagcct
    21301 ttgaaaacgc cgcaacggtt atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg
    21361 agtggctggt cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact
    21421 taggtttaac cccacagtca gtgaatattt tcggtggcta caaagttcag gggcgcggcg
    21481 atgaagcggg cgatcaactg ctcagcgatg cattagcctt agaagccgct ggggcacagc
    21541 tgctggtgct ggaatgcgtg ccggttgaac tggcaaaacg tattaccgaa gcactggcga
    21601 tcccggttat tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg
    21661 acgccttcgg cattaccggc ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa
    21721 cgggcgacat ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc
    21781 cgggcgaaga acacagtttc cattaaggag tcacgttgtg ttaattatcg aaaccctgcc
    21841 gctgctgcgt cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc
    21901 tactatgggt aatctgcacg atggccatat gaagctggtc gacgaagcca aagcccgcgc
    21961 cgatgtggtc gtcgtcagta ttttcgttaa cccgatgcag ttcgaccgcc cggaagatct
    22021 ggcacgttat ccacggacct tgcaggagga ctgcgagaag ctaaacaaac gtaaagtgga
    22081 tttagttttc gccccttcgg taaaagagat ctacccgaac ggtactgaaa cccatactta
    22141 cgttgacgtt cccgggcttt cgaccatgct ggaaggcgcg agccgtccgg ggcattttcg
    22201 cggcgtttcg actatcgtca gcaagctgtt taacctggtc cagccggaca tcgcctgctt
    22261 cggtgaaaag gactttcagc aactggcgct gatccgcaaa atggttgccg atatgggctt
    22321 cgatattgag attgtcggtg tgccaattat gcgcgccaaa gacggtctgg cgctaagttc
    22381 ccgtaacagt tatctgacgg cggaacaacg caaaattgcg cctggtctgt acaaagtttt
    22441 aagttcgatt gctgacaaat tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat
    22501 tgcggggcaa gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc
    22561 cgacacattg ctggaagttt ctgaaaccag caaacgggca gtaattctgg tagccgcctg
    22621 gcttggcgat gctcgcctga tcgacaacaa aatggtcgag ctggcgtaat acttaactgg
    22681 cgctacggct gatggcgcca gttattaatt taccccacgt catctgtcat aacacaggta
    22741 acttaaataa ggttaattac cttatcaatc tcagcaagag ataaatttgt catctctgca
    22801 acgtcttccc gagacattcc tttacttaga agacgcaggg cgaattcttg cctttcctcc
    22861 tgtcttccct gctgaatccc cttctcaatc cctttctctt caaaccactg cgccagcgtc
    22921 atcatagact cccctcccgt ttccctgtct ctcagcacac cataaaacaa atccgcttgt
    22981 tcagtatgac cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg
    23041 tacccttcgt caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga
    23101 atatgttttt gcagtagttc gagaatcgca atccgccgat gttgcatgat ttcgtcatcc
    23161 ggtgtgatgg tgatatccac cagcgggaaa ggactgttat agacgcgtcg tgccagctcc
    23221 ggcgagtaaa atatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga
    23281 taaaacagta tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc
    23341 atggcagcaa tagaataacg catcatgcga aaggccattt tcttatccgg cttgctttgg
    23401 tgttcaatca caacatgcag ataaccggga ttgccctgca tttgcacgga atagagcacg
    23461 tccgtgctgt gtcctttcag gctctcttca atgaaactcc ccgactctaa atgaagcgtg
    23521 ttgaggtcac aaagttctcg taattccact ggcaaatgta tctccagaaa gtcgcgagcc
    23581 gtctccgcat gcattaaaaa ttgtttaaat accgcgtcat gcggtgtggt actcggtgca
    23641 tccatcttcc ctgtcttccg tgttgtcttg cgtgaaggca gtatccagtc aaggcgcgcc
    23701 gtcgtcagcc gcctctttag tgtttaccga gcagcgttca gagaaatttc aattcgttac
    23761 aggcgataca tggcacgctt cggcgcggta cgcaaactgc tgacgcaaat gccatcagat
    23821 gagtagacac tcatcaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcacc
    23881 gcctgcttcg ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg
    23941 gcaaactcca ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca
    24001 ttgaccagga ttttcttgac gcagccggta ttctcgaaaa cgaagccatc gatatctgga
    24061 atgtcaccaa cggcaagcgt ttctccactt atgccatcgc ggcagaacgc ggttcgagaa
    24121 ttatttctgt taacggtgcg gcggcccact gcgccagcgt cggcgatatt gtcatcatcg
    24181 ccagcttcgt taccatgcca gatgaagaag ctcgcacctg gcgacccaat gtcgcctatt
    24241 ttgaaggcga caatgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcttgat
    24301 tgttatccct gcggctggtt actcaccagc cgcgacatcg tctccagcga atccgttctt
    24361 aagatataaa gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg
    24421 gttgtcaccg ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt gtcattaaat
    24481 ccgccaaacg gatacgaaag ataccagaca tgcggattaa attgcgccag agcgcggcgt
    24541 gaacgtgcaa aatcaaacag aatattgtgc tcactacggc tcagtaatat gggtcggcga
    24601 tagccatcca cccgatgcaa aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt
    24661 tcgttaagct cagaaacgct cataaattgc agcgattttg ggttccactt ctgcgggtga
    24721 cgtttgatgc gcgaggtaac aacaaacgcc gtcgccttca tgccatattg tttcaataca
    24781 ggatacgcat agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc
    24841 gcagggagat tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg
    24901 tatcccctgt cgcgcagcca ggccatctgg ttattgaaag cgcgtaccga ggtggtcgtc
    24961 gaagtatggc gaaaacgggt gttttcttca tcgcgcagaa tatggtgata ggttagcacc
    25021 ggcaggccat tatcgggttg ggcatccagc gcgctgatat aggcgagtcg atcgccaata
    25081 cggatctgat accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc
    25141 aaattgtccg ccagtacccc aaatggcgca ctgcccgcac tcggcgcgtt atagaccggc
    25201 gtatctttcc aggtaattaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg
    25261 tcttcaactt tttgtcgccc ctgaactggc tcgagatgcc ctttatcgat aaacccttta
    25321 ccaaagccaa aattaaatgc gtaataactt gcggcagtgg gctccacggc aatgatttgc
    25381 ccggcccgaa tattccccac ggtcaccatc ttgtcgccaa tttgcgccca gatcgcagca
    25441 ttttcgatgg tttgcatata acgggcaggt aacgcggcac tgacacttgc ggtaaacagc
    25501 atcagcagga gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc
    25561 ttttattgta gcaaaagcgc tataagaatc gtgggttaat actcatacaa attatgtttg
    25621 aggacgaaag gcggattttt ttgttgttgg tgccaaagac tactcacctc cggcccaccc
    25681 agttcgacaa tacgctcgcg aaactctgaa ctggtcatgg tttcacggca agccatcatc
    25741 tgttcaatta acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt
    25801 aataatgaag ccacgcggta cggtggcgct ccggctatat cggttaagaa gatgacaccc
    25861 tcaccagagt ccgcttcatg cagagcatcg cacatcatgc ggctgagcat attagagctt
    25921 aacccacgcc agaaattcac ggcccggcac tgaagaagcg ccccatgttt tttctccagc
    25981 gcatccagta tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc
    26041 ttcctcctta gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc
    26101 aaaactcctc tcccccaaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc
    26161 cacgttggat cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc
    26221 caaaggtagt gaccagcgga acatcattga taccgaggaa gccgtagcgg aaaccactga
    26281 tcatataaac gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca
    26341 gtgagtaaaa gaccccaccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc
    26401 tgatgtcatc gaacgttttg gcaaacacgc cgttcagcaa acccgcaagg gagaatagca
    26461 ccgccgtgag caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa
    26521 aaaacagtga aattgccgtc accagaatgc caacaaacag accacgcgcc acgccaccgc
    26581 cgacatatcc ggcaataatg acgtgagtcg gaaccggcgc taccagcagc tcttcaatat
    26641 tacgctggaa cttggcacca aaaaatgatg acgcgacgtt agcgtaggca ttggtgatca
    26701 ccgacatcat gatcagcccc ggtacgatga actgcatata gctgaagcca tgcatatcgc
    26761 caatacgcga acctatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg
    26821 gcggcaccag cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc
    26881 tttttagcgc cacccagtaa agatgcatca tgcgcgatct ccttgttttt cattaaccag
    26941 tgaaacaaac agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc
    27001 ctgctcactt aactgcgtaa atacgctgtt gatcccctgc tcacgcagca cttcaacttc
    27061 cagcgtcgcg gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt
    27121 cggtgcgaga tcgagaataa aggtttccga tttcagctta gccagcagcg ccttcatcga
    27181 ggtattttcc accagctcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc
    27241 ttcttccagg tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa
    27301 aaagccccac attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat
    27361 cagtagttta ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc
    27421 agataacata cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt taagatattt
    27481 ttcgctgcgg atgtacgcct ctttgcgctc cacaccgtag taccctgcct gatttaccac
    27541 aatttgctgc acggtttcaa acgggttgaa gttaaattcc tgcggcacca gtcccaactg
    27601 acgtttagcg ttcacgacat ctttctcgag atcgtaacca aatacgctga cccgcccgga
    27661 ggttttattt accagagagc tgataatacc gatagtggtc gatttcccgg ccccgttcgg
    27721 cccgagaagc gcataaaaat cacccgcttc gacctgcaaa tctattccac gaagcgcctg
    27781 aacgccgcct ggataagttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt
    27841 ttacttacct tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat
    27901 attagccaaa cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca
    27961 ctcatcagca acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt
    28021 gagaaactga cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc
    28081 gttcctgcag aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt
    28141 gctaacctgg tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat
    28201 gtactcgaag ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc
    28261 gccgttgaaa acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc
    28321 tggttcaaac atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg
    28381 tgtgaactga acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca
    28441 gcgtggaaac gcgggcagaa agttaccatt cacggctggg cctacggcat tcacgacggc
    28501 ttgctgcgtg atctggatgt taccgccacc aaccgcgaaa cccttgagca acgttactgt
    28561 cacgggattt ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga
    28621 tgcaacacat ccggcaactt cacacttact cgtccagcag aatcactttg ccgatatacg
    28681 gcagatgacg gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga
    28741 tcgagaaacc gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca
    28801 gcgtacaaat cgccagcgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca
    28861 gtgtattccc cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt
    28921 catccagatc tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg
    28981 aggcggtcat aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa
    29041 acataaatga gccacgcagc agacccacca gcaccatatc gctgccgctg tctttgtaac
    29101 gctcagtaat ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca
    29161 ttacttctac agtatgtttc atatctctaa ccatatgatt taaaaacaaa tcacttcgca
    29221 cgggcaggtt ttggcaccgc aacaaagcgc aagacacgca gtataccagt aaaactattc
    29281 attcgcggaa tctgttaata aagctcgaat tgtgatgacg atcacacatg ttaaaaccta
    29341 taattaattg ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca
    29401 atacaggctc gcgacgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc
    29461 tgtatctact cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta
    29521 tcgctggcct tgtgatgctc ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc
    29581 tttggctgta cgcagccctg ctgctcggca ccatgatttg gggcgtctgg gaagttggtt
    29641 tcgacttctg ggcgctgact ccgcgcagcg acattctggt cttcttcggc atctggctga
    29701 tcctgccgtt tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg
    29761 tggtggcact gctgattagc ggtggtatcc tgacctgggc cggatttaac gatccgcagg
    29821 aaatcaacgg caccttaagc gccaatgcca cacctgcgga agctatctca cccgttgccg
    29881 atcaggactg gcctgcctat ggtcgcaatc aggaaggtca acgcttttca cctctgaaac
    29941 aaattaacgc cgataacgtc cataatctga aagaagcctg ggtgttccgt actggcgatg
    30001 tgaagcagcc gaacgatccg ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg
    30061 acacccttta cctgtgtacc gctcaccagc gcctgtttgc gctcgatgcc gccagcggca
    30121 aagagaaatg gcattacgat cctgagctga aaactaacga gtctttccag cacgtaacct
    30181 gccgtggcgt ctcttatcat gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg
    30241 attgcccgcg tcgtatcatt cttccggtca atgatggtcg actgattgcg attaacgctg
    30301 aaaacggcaa actgtgcgaa accttcgcca ataaaggcgt gctcaatctg caaagcaata
    30361 tgccagacac caaaccgggt ctgtatgaac cgacttcgcc accgattatc accgataaaa
    30421 ccatcgtgat ggccggttca gttaccgata acttctcaac ccgcgaaacg tctggcgtga
    30481 tccgtggttt tgatgtcaac accggggagc tgctgtgggc ttttgatccc ggcgcgaaag
    30541 atccgaacgc aatcccgtct gacgaacaca cctttacctt taactcgcca aactcctggg
    30601 caccagcggc ctatgacgcg aagctggatc tggtctatct gccgatgggc gtgaccacgc
    30661 cggatatctg gggcggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg
    30721 cgctgaatgc cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt
    30781 gggacatgga tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag
    30841 tgccagttat ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg
    30901 gcgaactggt ggttccggcc ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt
    30961 acgttactcc gacccagccg ttctctgaac tgagcttccg tccgaagaaa gatttgagcg
    31021 gtgcggatat gtggggagcc accatgtttg accaactggt gtgccgcgtg atgttccacc
    31081 agatgcgcta tgaaggcatt ttcacgccgc catctgaaca gggcacgctg gtcttcccgg
    31141 gtaacctggg aatgtttgaa tggggcggga tttccgttga tccaaatcgt gaagtggcaa
    31201 ttgccaaccc aatggcactg ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc
    31261 cgatggagca gccgaaagat gccaaaggca cgggtacgga atccggcatt cagccacagt
    31321 acggtgtacc gtatggtgtc acgctcaacc cgttcctctc accgtttggt ctgccgtgta
    31381 aacagccagc atggggttat atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga
    31441 agaaacgtat tggtacgccg caagacagta tgccgttccc gatgcctgta ccggtgccgt
    31501 tcaatatggg tatgccgatg ctgggcgggc caatctccac ggcgggtaac gtgctgttta
    31561 tcgctgctac ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt
    31621 ggcagggccg tttaccagcg ggtggtcagg cgacgccaat gacctatgaa gtgaatggca
    31681 agcagtatgt ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact
    31741 atattgtggc ttatgcgctg ccggatgatg tgaaataacc ataatgcccg gagagatccg
    31801 ggcatatttc cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc
    31861 atgctccagc agatggcagt gcgccatata agcatgttct ttcggtgcgt tgtgattaaa
    31921 cttcaccagc acttcactga cattgccttc taccttaacg gtatctttcc agcccgcgcg
    31981 atgagccgct ggcggtttgc cattttctga caagatacgg aactgggtgc cgtgaatatg
    32041 gaacggatgc agcatcatgt cgcccacgcc agagataacc caacgttcgt actgcccttt
    32101 cgccgccgca aacatcggct tattcatatc aaacgcctga ccgttgattt tattggcatg
    32161 gtggaaatcg aacttcccgc catggttcat atgattcata ttgccgtgcc ccatatggcc
    32221 catcatctgg ctatgatcca tcccggccat cgcctgatcg ccatatttct ccatcagcat
    32281 ctgcatcccc atcatatcga gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag
    32341 cccttccagc gaaggtaacg caggcaggct acttaatgtg tctggcaaag caccggaggc
    32401 actaatagca atcggctgaa tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc
    32461 catccccatc tggctgaccg gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc
    32521 caccagcact tcaaaacgct cgcccatcag caccggcagc tcgctcacct tcaccggttc
    32581 aggtagcaga ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa
    32641 attgagcgag cgggcattac agccattgag caaacgcagg cgcagccaac cacgtggggc
    32701 agcgtgttgt gggtagattg caccgttggt cagcagcgta tcgccaaacc agcccacggc
    32761 ggcggtcatc acatccagtt gataatcaat ctgcccatca gcgttaaatt ttttatcctg
    32821 aacaatcacc ggaacatcat cgatacccca ctgttttggc agcattaatt tcaggatctc
    32881 gtcatcttca attaccacca gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc
    32941 atgttgatgt ggatggaacc agcaagtagc ggcaggttga tcaacgttca acgtcaccga
    33001 gcgcttgcca cctggcggaa taattccctg cgggccgccg tcgacttcac ccggtacttc
    33061 cagcccgtgc cagtgcaacg tcgtctcttc cgtcagttgg ttatagatat caaccgttac
    33121 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    33181 agttgcagtt ttctcgccaa aggtagactg acctgcgcca atagttaact gaatgcgatt
    33241 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    33301 tgcgcggctc cacagcggca aggctgaagc cacacccagc gcgacggaat attttaagaa
    33361 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    33421 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    33481 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    33541 atgaagacgt ttttcagaac agtgttattc ggcagcctga tggccgtttg cgcaaacagt
    33601 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    33661 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    33721 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagcc
    33781 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc
    33841 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    33901 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    33961 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggaatgaa
    34021 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    34081 accgatcacc aagatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    34141 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    34201 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    34261 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    34321 gatgcgggtg tcgtatcgtt ctgccgtcag tatctaccca accataacgc cggtagctac
    34381 gacgatccgc gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    34441 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctt
    34501 ttcacttcgg cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    34561 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    34621 agccattact tcagcgacgt tggcttttat caggcggcga ttccgaccta ttacggcggt
    34681 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    34741 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    34801 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    34861 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    34921 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    34981 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    35041 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    35101 gccagcgtca ctattctggt gagtgaagaa ccggtcgacc cgaaacttat cgacaaaaca
    35161 gaacaccccg gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc
    35221 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    35281 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    35341 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    35401 ggtatgaaac actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    35461 atgaaggcgc tgtatgacat ggtggatgta aatgtctatc aggaaaatat cttccatacc
    35521 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    35581 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    35641 tattacgggc gcaatatgcc agctgtttaa cgtctcgggc ggagctccca ggctccgcca
    35701 gatttattta cttctgctgc acgaaattac ggtaagccgc cacgacctgc agaaaatcct
    35761 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    35821 cgccagcgaa ttccagttga ttagcgcgaa ccatcacctc ttcaccgtcc atccacagag
    35881 tgtactcatg ccctgcccgc tgccaggaac gttcgctacc tttcagtgcg tgcgccgctt
    35941 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    36001 ccacttcatg ccccatggac atacgcacct ttacaactcc ggtaatatcg cgcagaaatt
    36061 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    36121 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    36181 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    36241 acttacttac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    36301 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgccacgta
    36361 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    36421 cgcagaagcc aggaagacat tcgcgccagt acccagacgg ttcgggaagt tacgggtaga
    36481 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagggaaca
    36541 gccagggatc tcgatacgcg caccactctt accgaagacg ctgtagtagc cttcttcagt
    36601 caactgcgcg gcgtccatac gggttggcgg tgccacccac aggcgggtcg gcagttggcc
    36661 tttgtgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttgg tcatgcagga
    36721 accgataaat acttcgtcga tcttctcacc ctgtaccgca gacagcggac gcgcgtcatc
    36781 cgggtcgttc ggtgcacaga ggattggctc tttaatatcc gccagatcga tgtcgatcac
    36841 tgccgcgtat tcagcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    36901 catgccctga atacgacgtt ccagggtgcg acgatcgccg taaccttccg cgatcatcca
    36961 cttcagcagg acgatgttag agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    37021 ggtacaacca gcggcagaac gctcggcgga cgcatcggtc agttcaaatg cctgctcaac
    37081 tttcagatcc ggcagacctt caatttccag gatgcggcca gagaagatgt ttttcttgcc
    37141 tttcttctca acggtcagca gaccttgttt gatcgcatac agcggaatgg catgtaccag
    37201 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    37261 catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg
    37321 gaaagagata ccgatcggga aacgggtatg ggaatcacca ccggtaccga cggtatccgg
    37381 cagcagcata cggttcagcc aggagtgaat gacgccgtca cccggacgca gcgacacacc
    37441 gccacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    37501 atacgccgcg gtgtggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    37561 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgggaac ctacagaagt
    37621 cattttcggt tcacagtacg cgcccggacg aatgcctttc acgccacagg cacggcctac
    37681 cattttttgc gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    37741 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    37801 aatcagcgga atacggccac cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    37861 gaaggtcgcc agcagttcgc cggtttcgtg gttacgcact tcacctttgt acgggtaaac
    37921 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    37981 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    38041 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttagtggc
    38101 ggatttacgc gaagaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    38161 ctgttgcaga gcttcgattt gcttgatcgg accaacaaca ccaggctggt ctggctcaat
    38221 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca
    38281 cgcatccggt gccggagaaa ggtcatcggt gttagtttcg ccagtgactt tgaagacggt
    38341 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    38401 ctgcataacc tgcttcgcat attcgttgcc tgctttcgct ttctcttcta cgtcatagaa
    38461 gttatcgaac atcagcagcg tgtgagaaag tgctttggca gcaataggtg ccagtttggc
    38521 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tgcccagcag
    38581 ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttcg cgatagcagc
    38641 caggaagcct gctttgacat aggcggcttc atcgacgcct gggggaacac ggttggttaa
    38701 cagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    38761 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    38821 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    38881 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    38941 acgggcagaa tagcaggatt ttgacgatgt gttaatctgt ttacaaaaaa gcaacataaa
    39001 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    39061 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    39121 ccgcctcttt atactcggat tcacagcacc tgcgggtggc agttcgccga ccattgcgat
    39181 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta
    39241 atttgcagtg ccgggctttg tgccgcctct accgggctat atataaaaag ccgcacagtg
    39301 gaagcgcctg tggaaccgca atcgacacaa cagactgcgc ctgacatcac cgcagttacg
    39361 cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgtcaaatc cgcattcagc
    39421 actgcacaaa tcgatcaatg ggttgcgcct gtcgcgctgt accccgattc tctgctttca
    39481 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    39541 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    39601 gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg cgaaaacccg
    39661 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    39721 gtacagcgat tgcggcaact ggcgcaacaa accggttcgc tgaagtcatc aaccgaacaa
    39781 aaagttatta ccacaacgaa gaaagctgta ccggtaaaac agacagtcac ggcacccgtc
    39841 ataccatcca ataccgtttt aactgccagc cccgtcatta cagagcctgc aacaaccgtc
    39901 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    39961 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    40021 gaaccgttta ttgacagctt tgtgcgcgga ttcggctata gcatgggtgt tgctaccacg
    40081 tacgcactat tcagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    40141 gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg gcaacacaac
    40201 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    40261 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    40321 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag cgccacgcag
    40381 cgacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    40441 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc acagcggttt
    40501 aatgaagctg aaaactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    40561 ctgacccagc aacaaaagga cgccgctcgt cagcgttatc agtcggcctc gcctgagcag
    40621 cgccaggcag tccgcgagaa aatgcagact aacccacaga tccagcagcg aagagacgca
    40681 gcgcgtgagc gtattcagtc agcctcgcct gagcagcgcc aggtgtttaa ggaaaaagta
    40741 cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtgt tcaatcagca
    40801 tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg agagccgccc acaacgtcta
    40861 aacgacagta accatactgt caggctgaat aacgagcaac ggtcagcagt acgcgaacgt
    40921 ctctctgagc gcggagcaag gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg
    40981 tggttagccg cttttttaat tgccggatgt tccggcaaac gaacaattac ttcttcttcg
    41041 ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc aggcccacag
    41101 actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca
    41161 tttcgattgc caggccgatt tcacccagca gctcgccgcc gttggtaccg acaatcgcac
    41221 caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg tctgcgcagt
    41281 cggaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca tagctgatgc
    41341 ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt tcggtatagg
    41401 cgatggacgg gataactttc ggatcgaagt agtgtttctt accggcgata acttcagcgg
    41461 caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccgacg atatcgccga
    41521 tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg aaaccacggt
    41581 cgtccacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc acacgaccaa
    41641 tcgctaccag cacggcgtcg taacgctgcg gttcagcggg tgcttttttg ccttccatcg
    41701 tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt
    41761 tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca gctgccggga
    41821 taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc
    41881 ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt acttctttca
    41941 gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata aacggcagtt
    42001 ggatcgggcg agaacccgct gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt
    42061 tctcaccttc aacttccagg gtgttagccc cggtgaattt acccagaccg ttgaccactt
    42121 tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg atcactttct
    42181 ctttccaggt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt
    42241 cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct ttagaaggga
    42301 tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg attacggttt
    42361 ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct gccccaagta
    42421 ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta tctccggcgg
    42481 tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac cccagtcact
    42541 tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc agaaatccat
    42601 agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca
    42661 aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg ctaataacct
    42721 caatacattc atgagattac cagaaaaaag ccggccgttg ggccggctct tttacttaca
    42781 tcaccaggcg gcgaatgtca gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat
    42841 cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat cggcagcatc agacgcggca
    42901 cgaactcttt accattccac accggctcca tcgcggactt ggaaacgccg aggatagcca
    42961 cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc caggccgccg atgctggaga
    43021 tggtgaagca accgccctgc atttcgcccg cagtcagctt accgtcacgc gctttcttag
    43081 aaatagtcat cagctcgcga gacagctcga tgatgccttt cttgttgacg tctttgaata
    43141 ccggaacaac cagaccgttc ggggtatcca ccgccacacc gatgttgatg tatttcttca
    43201 gggtcagacg ctgaccgtct tccgacaacg aactgttgaa gcgaggcatc tgctcaagag
    43261 ctgcagcaac ggctttcatg atgaagacaa ccggggtgat cttcacatcc agcttacgtt
    43321 tcgccgcttc ttcgttctgc tgtttacgga acgcttccag ctcggtgata tcggttttgt
    43381 cgaagtgagt aacatgcggg atcatcaccc agttacggct caggttcgca ccagaaattt
    43441 tctggatacg gcccagttcc acttcttcga tttcaccaaa cttgctgaag tccaccttcg
    43501 gccacggcag catgcctggg ataccgccgc cagtcgccgc cggagctgct tctgcacgtt
    43561 tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag gatacgacct ttacggccag
    43621 tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc cagacggcgg atcagcggag
    43681 tcgcgtgaac gtaagcgtcg ttttctgcaa attcagattt gccttccgct ttcgcagccg
    43741 gtgctgctgc cggagcttca gcttttgctg ccggagccgg cgctgccgct tcctgtttcg
    43801 caggagctgc cgcaggcgct gcgccttcaa cttcgaagat cataatcagc gagccagttt
    43861 tcactttatc gccaacgttg actttcagtt ccttcacgac gcctgcaaac ggagccggga
    43921 cttccataga agctttatcg ccttctacgg tgatcagtga ctgttcagcg gcaattttgt
    43981 cgcccacttt caccatcact tcggtcactt caacttcgtc accgccgata tccggaacgt
    44041 taacttcttt cacgccagcc gctggtgcag gggccgctgc cggagccgct tcctgtttag
    44101 ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa gaccataatc agcgagccgg
    44161 tagacacttt gtcacccacg ttcactttga tctctttcac ggtgccagca aacggagccg
    44221 gaacttccat agaagcttta tcgccttct
//
LOCUS       DAHBHC010000037        43531 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHBHC010000037 DAHBHC010000000
VERSION     DAHBHC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.037"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..357)
                     /locus_tag="M2A35_002439"
     CDS             complement(<1..357)
                     /locus_tag="M2A35_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HCB7198730.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSL"
     gene            complement(354..2621)
                     /gene="ycjT"
                     /locus_tag="M2A35_002440"
     CDS             complement(354..2621)
                     /gene="ycjT"
                     /locus_tag="M2A35_002440"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCB7198731.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTNQEDE"
     gene            complement(2618..3673)
                     /locus_tag="M2A35_002441"
     CDS             complement(2618..3673)
                     /locus_tag="M2A35_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCB7198732.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(3683..4471)
                     /locus_tag="M2A35_002442"
     CDS             complement(3683..4471)
                     /locus_tag="M2A35_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCB7198733.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(4489..5541)
                     /locus_tag="M2A35_002443"
     CDS             complement(4489..5541)
                     /locus_tag="M2A35_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCB7198734.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(5572..6414)
                     /locus_tag="M2A35_002444"
     CDS             complement(5572..6414)
                     /locus_tag="M2A35_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCB7198735.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6401..7282)
                     /locus_tag="M2A35_002445"
     CDS             complement(6401..7282)
                     /locus_tag="M2A35_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCB7198736.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7303..8595)
                     /locus_tag="M2A35_002446"
     CDS             complement(7303..8595)
                     /locus_tag="M2A35_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7198737.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(8609..9861)
                     /locus_tag="M2A35_002447"
                     /pseudo
     CDS             complement(8609..9861)
                     /locus_tag="M2A35_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10074..10388)
                     /gene="pspE"
                     /locus_tag="M2A35_002448"
     CDS             complement(10074..10388)
                     /gene="pspE"
                     /locus_tag="M2A35_002448"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCB7198738.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(10463..10684)
                     /gene="pspD"
                     /locus_tag="M2A35_002449"
     CDS             complement(10463..10684)
                     /gene="pspD"
                     /locus_tag="M2A35_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCB7198739.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(10693..11052)
                     /gene="pspC"
                     /locus_tag="M2A35_002450"
     CDS             complement(10693..11052)
                     /gene="pspC"
                     /locus_tag="M2A35_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCB7198740.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(11052..11276)
                     /gene="pspB"
                     /locus_tag="M2A35_002451"
     CDS             complement(11052..11276)
                     /gene="pspB"
                     /locus_tag="M2A35_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCB7198741.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(11330..11998)
                     /gene="pspA"
                     /locus_tag="M2A35_002452"
     CDS             complement(11330..11998)
                     /gene="pspA"
                     /locus_tag="M2A35_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCB7198742.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12165..13142
                     /gene="pspF"
                     /locus_tag="M2A35_002453"
     CDS             12165..13142
                     /gene="pspF"
                     /locus_tag="M2A35_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCB7198743.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13262..14527)
                     /gene="puuE"
                     /locus_tag="M2A35_002454"
     CDS             complement(13262..14527)
                     /gene="puuE"
                     /locus_tag="M2A35_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCB7198744.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(14565..15845)
                     /gene="puuB"
                     /locus_tag="M2A35_002455"
     CDS             complement(14565..15845)
                     /gene="puuB"
                     /locus_tag="M2A35_002455"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCB7198745.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(15847..17334)
                     /gene="puuC"
                     /locus_tag="M2A35_002456"
     CDS             complement(15847..17334)
                     /gene="puuC"
                     /locus_tag="M2A35_002456"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCB7198746.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(17609..18166)
                     /gene="puuR"
                     /locus_tag="M2A35_002457"
     CDS             complement(17609..18166)
                     /gene="puuR"
                     /locus_tag="M2A35_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCB7198747.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18193..18957)
                     /gene="puuD"
                     /locus_tag="M2A35_002458"
     CDS             complement(18193..18957)
                     /gene="puuD"
                     /locus_tag="M2A35_002458"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCB7198748.1"
                     /translation="MENIMNNPVIGVVMCRNRLKDHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19169..20587
                     /gene="puuA"
                     /locus_tag="M2A35_002459"
                     /pseudo
     CDS             19169..20587
                     /gene="puuA"
                     /locus_tag="M2A35_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
     gene            20736..20900
                     /locus_tag="M2A35_002460"
     CDS             20736..20900
                     /locus_tag="M2A35_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198749.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            20890..22275
                     /gene="puuP"
                     /locus_tag="M2A35_002461"
     CDS             20890..22275
                     /gene="puuP"
                     /locus_tag="M2A35_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCB7198750.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWINLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22409..22654
                     /locus_tag="M2A35_002462"
     CDS             22409..22654
                     /locus_tag="M2A35_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCB7198751.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            22967..24610
                     /gene="sapA"
                     /locus_tag="M2A35_002463"
     CDS             22967..24610
                     /gene="sapA"
                     /locus_tag="M2A35_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCB7198752.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            24607..25572
                     /gene="sapB"
                     /locus_tag="M2A35_002464"
     CDS             24607..25572
                     /gene="sapB"
                     /locus_tag="M2A35_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCB7198753.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            25559..26449
                     /gene="sapC"
                     /locus_tag="M2A35_002465"
     CDS             25559..26449
                     /gene="sapC"
                     /locus_tag="M2A35_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCB7198754.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26449..27441
                     /gene="sapD"
                     /locus_tag="M2A35_002466"
     CDS             26449..27441
                     /gene="sapD"
                     /locus_tag="M2A35_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCB7198755.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27443..28249
                     /gene="sapF"
                     /locus_tag="M2A35_002467"
     CDS             27443..28249
                     /gene="sapF"
                     /locus_tag="M2A35_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCB7198756.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            28317..28670
                     /locus_tag="M2A35_002468"
     CDS             28317..28670
                     /locus_tag="M2A35_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCB7198757.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVLVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            29038..29826
                     /gene="fabI"
                     /locus_tag="M2A35_002469"
     CDS             29038..29826
                     /gene="fabI"
                     /locus_tag="M2A35_002469"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCB7198758.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            29971..31098
                     /locus_tag="M2A35_002470"
     CDS             29971..31098
                     /locus_tag="M2A35_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCB7198759.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLYVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETHSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKIRPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            31166..33100
                     /gene="rnb"
                     /locus_tag="M2A35_002471"
     CDS             31166..33100
                     /gene="rnb"
                     /locus_tag="M2A35_002471"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCB7198760.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRITLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(33178..33282)
                     /locus_tag="M2A35_002472"
     CDS             complement(33178..33282)
                     /locus_tag="M2A35_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198761.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            33336..35321
                     /gene="pdeR"
                     /locus_tag="M2A35_002473"
     CDS             33336..35321
                     /gene="pdeR"
                     /locus_tag="M2A35_002473"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCB7198762.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            35468..35641
                     /locus_tag="M2A35_002474"
     CDS             35468..35641
                     /locus_tag="M2A35_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198763.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            35731..36480
                     /locus_tag="M2A35_002475"
     CDS             35731..36480
                     /locus_tag="M2A35_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCB7198764.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            36749..36967
                     /gene="osmB"
                     /locus_tag="M2A35_002476"
     CDS             36749..36967
                     /gene="osmB"
                     /locus_tag="M2A35_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCB7198765.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37093..37419)
                     /gene="yciH"
                     /locus_tag="M2A35_002477"
     CDS             complement(37093..37419)
                     /gene="yciH"
                     /locus_tag="M2A35_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCB7198766.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37419..38156)
                     /gene="pyrF"
                     /locus_tag="M2A35_002478"
     CDS             complement(37419..38156)
                     /gene="pyrF"
                     /locus_tag="M2A35_002478"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCB7198767.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(38349..39518)
                     /gene="lapB"
                     /locus_tag="M2A35_002479"
     CDS             complement(38349..39518)
                     /gene="lapB"
                     /locus_tag="M2A35_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCB7198768.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(39525..39833)
                     /gene="lapA"
                     /locus_tag="M2A35_002480"
     CDS             complement(39525..39833)
                     /gene="lapA"
                     /locus_tag="M2A35_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCB7198769.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(39982..40746)
                     /gene="pgpB"
                     /locus_tag="M2A35_002481"
     CDS             complement(39982..40746)
                     /gene="pgpB"
                     /locus_tag="M2A35_002481"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCB7198770.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            40916..41506
                     /gene="ribA"
                     /locus_tag="M2A35_002482"
     CDS             40916..41506
                     /gene="ribA"
                     /locus_tag="M2A35_002482"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCB7198771.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(41570..>43531)
                     /gene="acnA"
                     /locus_tag="M2A35_002483"
     CDS             complement(41570..>43531)
                     /gene="acnA"
                     /locus_tag="M2A35_002483"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCB7198772.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10274 a        11517 c        10972 g        10768 t
ORIGIN      
        1 cagggagaca gaagccagcc caaccgggat ctgctgtgca cggagatctg ccagcaaaga
       61 gcgaatgccg ggtagaacag cgctgaccgt caactcgcgc agtgagtgga catagagcag
      121 atttttgcga tacgccagtt gcgccctctc ctgcgagtta aagtcgccct ctttgccccc
      181 gtgttgcaga atgcgccgca gagactcatc gcggctgatc ccttttaggg attcgttaaa
      241 ctgcgcatca atgctgatgc caatttcagc ggcaatctgc tgccacgcct ggaaatgcag
      301 atgcgcggta tcggtgatta caccatccag atcgaaaatt accccttgca gtttcattca
      361 tcctcctgat ttgtggtagc ggtcccattg aagggcaaaa taaaatcacc caaacagaaa
      421 acagattctt cagccacgga tataagctga ccgttcaaac gcagtgaaac gggcgcagaa
      481 gttcgaatcg caatacgctg cgcgtcaaga gtgacctgta attcgcagcc ctgccagaac
      541 agagggaaag acaactgttg ccactgctca ggtaacgccg gattgagatg caattcaccg
      601 tcacgcacgc tcaccccggc aaaaccctga atcgccccca gccagatagc gccagttgcg
      661 gcagcatgga taccatcatc acaactatgc ggatcagcac caagatcgat ttcagtcccc
      721 tcgcgccaga actgataact ttgggtcagc aggccacagc gtgcggcaac aatgccgtgg
      781 attgctttac ttaatgacga gtcgtgaata gtgcgcggtt cataaaattg cagattggca
      841 agacacgatg ccgctgagaa ctgctccggc agcatgtaat tgagcatcac cacatcagct
      901 tgtttgagga tttgcatctc gttcacttct gcgcgtgaat aatccagcag aatggtttgc
      961 ttccccgccg ccgctttgta tttcgccaga ttaatcgccg gcttagccat aaacgaatca
     1021 tcctgcggca aaacgccgtc gggctgaatt tctggcagcc gcagttcttt aaggaacatt
     1081 tcggcgcgat ggataaacgc atcgtcgcta cagccaaact ggcgggcaat actcagcgcc
     1141 tgctggacgt tgtaatacgc catatagctg gtgaaggcgt tattattgac atgttcggta
     1201 tattcgtcag gcccaataac atcatgaatt tccagacggt cgttaaccct caccgcgcgg
     1261 ctaatccaga actttgcagt ttccagaagt agcgccatgc cttcatgcgc aatgaaactt
     1321 tcatcccccg tggtctgcca gtattgaata accgcccagg cgatatcggc caccagatga
     1381 tgttccgcct gcgccgaggc cactttttgc cgcagcccgg tgcgaatgtt aatggcggca
     1441 aattccggcg tctcttcttc gccgctgcgc gcgctttccc acggaaatag cgcgccctgc
     1501 cagccgttgc gtcgcgcttt ctcctgcgcg cctggcaagt tgtgccagcg ataacgcagt
     1561 aaacttcggg caaccgtcgg atcgctaaac agatgaaacg gtaacaaaaa tacttctgta
     1621 tcccagaaaa cgtggccttt gtagccttcg ccggttaagc cttttgccgc aatgctgctg
     1681 cgctcgtcgt gggcaggcgt catgatgcgc agatgataaa gcgcataatc taacgcttgc
     1741 tgatcgtgcg cttcgccgcc atttaccgtg atacgacgtt tctgccacca ttgacgccag
     1801 ttttctgttg atactgcaag aagttggtcg taactctgct gcgcgcacat ttcaagctgg
     1861 cgaagcgacg cgctgcccca ctcgtctaaa acggcttgcc tgtcatcccg ccagtcgatc
     1921 cacaccagtt tttgcaacgt cactgtctcg cctgcatgaa gctgcgcact ggtatgttgc
     1981 tgcaaacggc gctctttggc ggtatagcat tgctgcacat caccgctcac catacagcaa
     2041 cagctgatgg ccacatcact gcgtccatcc tgggtggtgt agatcccctg catcagatgc
     2101 tgaccaaaca cccgcacctg ggtttcgtcg agatgttggc gaccgtggtt ggtttgcgtg
     2161 gcgtcgatac ctgttgaaat cagcactgac gcgtcggcgt ccagtggcgt aatagtgatt
     2221 tccagcacaa tgagcggcag ttggtctgcc gaaacaaagc gacggctggc gatggtgtaa
     2281 cctgtgccgt tgctggtacg ccagacaacg ctacggcgta attctccact ggcaaagtca
     2341 agttcacgct gccaggcttc gcgggataac gagaaaacct caccatttat ggcaatctcc
     2401 atccccaaga tatcaggcag gttcaccagt tcgttgattt caccttttcc cgcccgatga
     2461 tacagccccg ccagatacat ccctcgcgtc tggcgcgtgt aatcttcttc atggctggcg
     2521 cgaagcccaa gatagccgtt cccctgcgcc atcagcgatg catacttgtt cagggtatgc
     2581 tggctgaaat ggggttctga taacgttact ggcctggtca taattccaca cgcgtccctg
     2641 tttctgcgga ttgatacaac gccgccacca gttgctggat gatgtacccc tgctcggcgt
     2701 cggctatcat cacgggcttg ccctgtacgt gattgataaa ggcttccatg ctgcgcaaat
     2761 ggcggttgtc gtctgccagt tcccgttgca tcagcgtcat taattcaccg ttgttatcgg
     2821 tgtagatatg tgctggaaac agcgtcgcac cggctttatc accacaaaag ctgacgttca
     2881 taatcgactg ttcgcggatg ttgagtgcaa atgacgtttc cagccacaga atgccgccgt
     2941 tatgaaattc aatggtgcca aataacgaat cttcgacgct gtaagttgcc ggatcccact
     3001 cgccaaattg accgcagctc ttttgcgtgc cgatcttttg aaagctatgc gcattcacgc
     3061 ttttcaccgc cggaaagccc agcacataca tcgccgcatc cagcatatga atgccgatgt
     3121 cgataagcgg gccaccaccc tgcagttctt tattggtaaa gacaccccag ccgggaacgc
     3181 cgcagcgacg cagggcgcgg gcggtggtaa cgtaaatctc tcccaaaacg ccgttggtca
     3241 cctgttcacg cagctgttgc gtatcgagcg caaaacggtg gtgaaagtcg taggccagca
     3301 ccttgcccag tttgcgcgcg gtatcgcaca tttcccgcgc ctgttctggc gtcatggcgg
     3361 gcggtttttc gcacatcaca tggcagcccg cttccagtgc catcagggta tgttcgtaat
     3421 gaaaacggtt aggtgagcag acgctaacca catcaggttt caccgccagc agcatggcct
     3481 gcggatcgtc ccacacggat gcattaccgt atttatctgc cagcgcctgc gcctgggaaa
     3541 ggcggctgtc acagacagcc accagttcca gatcgttgcg ggtgcagtag tacgaagcat
     3601 gaaccttatc cgccacctgg cctgcgccta ttatcgcgac ccgcagcgga gagcttgtca
     3661 ttgcactttt cacaaagacc tcttagcagg tacgcaacca ggccaacgaa tcacggtacg
     3721 cctgggcagg atcttccgcc cggatacgcc cttcgtacac tacatagccc tgatagttat
     3781 ccgcgcgcag ctgttcaaac agcgcgtgga aatccagggt gccgctgccc ggctggtagc
     3841 gatggttatc cgcaatatgc acatgaccga gcaggtcgcg gttgtcatgc agcgcctgcg
     3901 ccaggttatc ttcttcgata ttcatgtgat agaaatcgcc gataatctgt acatgtttaa
     3961 gatcgttttc gacgatgtaa cggcgggcat cggcgagggt gttgatcata tgatcctgat
     4021 agcggtttaa cggttcgaga tacaccacgg ttccggtacg cgcggcgacc tgttccagta
     4081 cgtgcaggga atcactcacc attttgcggt cgccgtccag gctacgcggc gaggtcatcg
     4141 gcggtaagcg gaaggtaaac atgccccacg ctgccggaac gacgatacct ttacccccca
     4201 cttctgccag cgcttcgaga atgcgttcga tctgctttaa gccattaaga cgacgctctt
     4261 cgataaagtc gccaatccaa ccgtcatagc caccgcaggc ggtggtcacc ggtaaaccgg
     4321 tttctttgat tgctgctttg acttcttcga gattgttgac cagcagtttg ccgtcaatct
     4381 caaaaccatc gaagcccatc tctttgatat aacgaaattt ctccagaatg ttttccggaa
     4441 agaatgcctg attctgtgtg cctattttca ttttttcatt cctttgaatt aaaacgtaac
     4501 gcccattttg atgctctgtt ccggatgctg atcgacatac tgcatgtagc tttccgggct
     4561 cttggcaaag gtcactaccg ggtcgattaa atcttcgcaa ttgagataac cgttcatcag
     4621 cagttcccag caggtttctt caatacgctt acggctccag cgcggataat ccgggttcgg
     4681 ttcgctgcac gcacgggaga aaacaatttt ggcgttattg aaatgcgctt cgcgtccgag
     4741 gttaaaacct tcagcaaatg gtttggcaaa cgcgacataa gagatggtgc cgccataagc
     4801 aaggccacgc agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga taatcacatc
     4861 ggcaccctgc ttgccggtca gcgttttgat ctcttttcct acgtcagtgc caatggggtt
     4921 aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag cgataggcga taggatcgac
     4981 gccaatcacc accgacgcgc cagcgcgttt cgccagttgg atggctattt gaccaatcgc
     5041 gccaagccct accaccacca caaaatcacc cacgcgcacg ttggcatcgc gcacgccgct
     5101 catggcaaac tgcgccgggt cgtaacaaac ggcgtttttc catgagctgc cttccggcat
     5161 tttgcgcagc ttgtagttat tcaccgcgtt aatgatgacc gtctcggaga gcgggccgta
     5221 gccgcatacg ctgtcgccca ccgcgtagtc ggtaacgtcg ctgccgcact cgataatgtc
     5281 gccaaccacc atgttgccaa gctggaattt gccaaactca atgccgcgcg gcgcatctgc
     5341 cggacgcgga gtgaacatct gccattcgcc gttaaagtct tcatcgataa acgggctggc
     5401 ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca cgcggatttt
     5461 cacttcattg gctaaaatcg cccgatcttc atactcaacc agcgcagcaa cacgcggtgc
     5521 tgtggctact aactttttca tttaaaactc cttgtaaaga aactggccgc tttagccctt
     5581 cacgccaccg gcggtcaaac cactcttaat aaaacgttcc gaaagcgcat acataatcac
     5641 caccggcaat gcggtcacca gtgaggccgc catcatccgt ccccagatgt aatctggcgt
     5701 actgaacagc gcgttcaggc ccaccggtaa ggtgaaattg ctggcgctgg agaggaaaat
     5761 cgacgcaaac agatagtcgt tccacgccac cataaagcag tagacgaaca cggatatcag
     5821 cccggacatc gccaacggca cggtgatgcg gaagataatt tgcagacggt tgaggccatc
     5881 catcatcgcc gcttcttcga tctcatcggg gatggtgtcg aagtagcttt tcagcataaa
     5941 cacggcggtg ggcagagttt gcgtcaccat ggtgatgatc agcgccatct cggtgtcgta
     6001 aatgcccagc gcggtgatga ttttgaacag cggcaccacc agcaaaatgc cagagaacat
     6061 atacaccgtg taaaagctgg cattgatggt catccgacct ttaaagcgca ggcgggaaag
     6121 cgcgtaagcc ccaagaatgc cgaggaatac cgccaccacg gatgaaacca ccgacaccac
     6181 cagactgtta cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacgta
     6241 atgctccagc gtccactgct gcggcagcag cgtaggatgc agtgagatcg cctctttcgc
     6301 gcccttgaac gaggtcatca gcatcacaaa aaatggaaac agcgtgatga tgagaaacag
     6361 cgccagcccg cagtaaaaac cgatgcgact gagtgtgcgt ttatttgttg ccattgaggt
     6421 tcacccgttt cctggtcagc agaatgacag cgaaaataat gatgaagagc accaccgaga
     6481 tcgccgccgc tttccctaag tcattaaagg caaaggcggt tttgtagaga tatacaccga
     6541 gaatatcgac cttggtcgtc agcaaataaa catccgcgaa catgtagaac atccagatgg
     6601 tgcgcagggt caccaccgtc gccaggaccg gcataatcgc gggcagcgtg acgatacgaa
     6661 accgttgcca ggcattagcg ccatccatct ctgcagcttc gtacagcgat ttgtcgatag
     6721 tctgcaaaat cgccagaaac gagataaagg catacgggaa gtagcgccag atggcgaaca
     6781 gcaccaccag cacaaagcta ctgcccggat tatcgaacca cagcggtgcc tgctcataga
     6841 gatgcagaag atcgacgccg aggtagttca caatgccgta gccgttgttg aacatgtatt
     6901 tccaggcaaa caccagcgaa atggacggcg ttacgtagga gaggatcacc agcgaacgcg
     6961 cggttttgcg cagatggaac tcgcggttga aaaacatcgc cactgccagc ccgagcacgg
     7021 tgctgcccgc caccaccagc gcggtatacc agaccgtcat ccacagcgag tgccagaagc
     7081 cgggatcgga gaggatacgc acatagttac tcaccccaac aaaggttgac tcgatgttgg
     7141 gattgagcgg cagacgtaaa aaactgattt cgatattcga caccattggc caggccacca
     7201 gaccacccag cagtaataag ctgggcgcga gaagcagcag cgcaaagggc atatcggaac
     7261 gacctgaaaa aagcctgttc attgcggatt cctgtcctgg gtttagtgct gttcgatcag
     7321 ttcatccagc tttttctggc tcgcttgcag cgtgccaggg agatcggctt tacccacggt
     7381 gacgttatgc accatggagc tcaccactcc agaccccgtc acatcaccca tgcgggtaaa
     7441 gtttttatcc cctactgcac caaagacctg aatatttggc agttcaccga ttagctgatt
     7501 cggtagttca cccagcgcct taataacgtc gttgtctttc caggtggcgg tagtgacaac
     7561 cgctttattc accggcagcg cagcacctgg cgacatcatc acccagtcgg caatgttgtc
     7621 tgcctgctcc ataaaggtga caaatttttc tgctgcttcc gtctcttcgg tcttttgccc
     7681 ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct cggttggcac
     7741 cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga tataggtgga
     7801 gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt
     7861 agaacccggc atagtgttgg cagcaaggtc gcggtaatag gtcagtgcct gcatcatctc
     7921 tggtgtatca agggtgattt tgccttcggc gttaaagacg ttagcctggt tggataacgc
     7981 aaactgggag aaggattgtt ccgtcaacac gctttctgct gtaggcagcg caatgccgta
     8041 ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt gccagttttt
     8101 cggctcctca agccccgctt ttgccagcac atctttgcga taccagatac cgccaatcca
     8161 ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa tacgcagtac
     8221 gccatcgtaa aacgcgcctt caccaacgtt actgatgact gtggcaaccg ctttgcgatc
     8281 gataagctgc tctttgtcca tcactttggc gtagtcatgg ctggtttcga tcacttccgg
     8341 cagcgaaccg ctacgtgaaa gagtaatgac tttagtgtta taggcatctt cttccacggg
     8401 cacttgctta acggtgatgc cagggttttc tttttcaaaa cgggcaatca atttactgat
     8461 aacggcctgg cgctcctgct ccaccgaaga atgcataaat tcgatggata cattcgtttt
     8521 attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata acagcacgat
     8581 ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc caggggcgca
     8641 gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca tgcagattag
     8701 taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga caattaccat
     8761 cagcgttact tctttgaata cgcattacgg atgaattaat cgtatcgatg gtaaaattat
     8821 tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat
     8881 ataccgcatg ccttaatgta gcttcatcgt tcagttctcg ggttatctct ttactgtgat
     8941 attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac
     9001 gcgagccaag aatactttga atatatatcg ctggcacacc ggggaaactt aacaaaatcg
     9061 catgggcgag aataaacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
     9121 cagatgtgtt ttttccagat ggatgcagct ttttcccggc tctttccaca taaagccaac
     9181 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
     9241 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
     9301 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
     9361 cgggcgagtg acggcgctga gatcggtttg cgggtcaacg gcaataaaaa aatcttcaaa
     9421 acctggatgc tgttgtttaa accattcact ttttgccgac atatggttgc agacaaaatc
     9481 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
     9541 ggcgacctga tgataatcaa ttaccgaaaa gccatcatca gatgaccatg gataaaacgg
     9601 caacaaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact gattaaatgt
     9661 gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta acacgacatc
     9721 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     9781 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccaggta
     9841 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga aacgagagac
     9901 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     9961 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg ggatgtaaat
    10021 aaggtgtgac agaaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct
    10081 ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc
    10141 atctcgctaa ggatctcttt tgcttgccct gactggcgtc cggcattgca atacactttc
    10201 acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga
    10261 atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc
    10321 cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt
    10381 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
    10441 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
    10501 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
    10561 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
    10621 gcttacctgc taatttgaaa ccaggcttta ccttttgccc ggcctgttgc cagcgagtat
    10681 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
    10741 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
    10801 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
    10861 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaagaaaat
    10921 cgacagcacc accaggatac gcaccagttt tgccggtaca tcaaaatagt tggcaatccc
    10981 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataattttt tattgagatt
    11041 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
    11101 gcctgaatac gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa tcgctgctgc
    11161 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
    11221 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
    11281 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
    11341 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
    11401 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
    11461 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
    11521 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
    11581 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctca ccgatctctt
    11641 tcttcatgcg tgccagcgtg tcgtccacca gcgtcacttc atgttccagg ctcttaatca
    11701 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
    11761 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
    11821 ggcgagtcag ctgtttcttt tctgccaacg cgcgcgccga agtagaacgt acttcaacca
    11881 gtgtatcttc catctcctgg atcatcagac gaaccagttt ctgtggatct tccgctttct
    11941 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
    12001 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
    12061 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
    12121 caggatgagt tagcgaatta cactaacaag tggcgaattt catcatggca gaatacaaag
    12181 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
    12241 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagctgattg
    12301 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
    12361 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
    12421 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
    12481 ttgatgaact cgctacggca ccgatgatgg tgcaggagaa attattgcgc gtgattgagt
    12541 acggtgaact ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg cggttggtat
    12601 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg cacttttcgc gctgacctgc
    12661 tcgaccgact ggcttttgat gttgtacaac tgccaccact gcgcgagcgc gaaagcgaca
    12721 taatgttgat ggcagaacac tttgccatcc agatgtgtcg ggaaatcaag ctgcctctgt
    12781 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
    12841 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
    12901 cgcttgatga catcattatt gatcccttta aacggcgtcc gcctgaagac gctatcgccg
    12961 tttcagaaac cacctcgctt ccaacactgc cgctggattt acgtgagttt cagatgcagc
    13021 aggaaaaaga gttgctgcaa ctcagtttgc aacaagggaa atataaccag aagcgcgcgg
    13081 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
    13141 agcgaagttg agcaatacaa cttgcaggat gcggcgtgaa tgctttattc ggcatacata
    13201 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
    13261 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc acatccggga
    13321 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
    13381 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggcttgcccg
    13441 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
    13501 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
    13561 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
    13621 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
    13681 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
    13741 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
    13801 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
    13861 cggcaaccag ctcttttggc gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa
    13921 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
    13981 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
    14041 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
    14101 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
    14161 cgcgagcaat tttcaccgcg ttttccaccg cttccgcacc ggtagtgaag aatgcggttt
    14221 tagcctgccc gctcactggg gcaagggcgt taattttctc cgccagcgtg acgtagcttt
    14281 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
    14341 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
    14401 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
    14461 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
    14521 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
    14581 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
    14641 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgttcggc gtcgccgcgc
    14701 agcagttcgg caatcagacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtag
    14761 ccctgcatgt aatagatgtt ggtatcaagg cgaccgaact gcggcatgcg cgacaaggtc
    14821 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcagctg cgggaaggtt
    14881 ttcagcagtt tcggcaccac aaggcgctca acgtcatccg ggtcgcgcgc gccgtagacc
    14941 acgccgccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
    15001 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
    15061 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgccag ttccggctct
    15121 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacct
    15181 ttggcagttc gcacaacggc tggcgtggtg tgctggatct gcgtcacggc agaaagttca
    15241 tacacgcgcc cgccgttgag gcggatggcg tccgcttcgc caatggcgag gtttagcgga
    15301 tgaatatgcc caccgctgtg atccagcagc gcaccggtat agcgatcgct ggctacttca
    15361 cggcgaatgg cgttggcgtc cagcaattcc agctgtttat tgccgtagcg ttcccagttc
    15421 tctttctgct cttcaagtgt ggcgagctgt ttatcattca tcgccacaaa caggccgccg
    15481 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcaccacct
    15541 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
    15601 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
    15661 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
    15721 gaagagagcc cggtatagcc gccgccgacc acgcaaacgt cgcaggaaat cgactcattc
    15781 agcgtgtcga atggcgcgta tttattcgca ctggcggcgt agtaactgct ggtatgttcg
    15841 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcggtgaatt tttcaagggc
    15901 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
    15961 atcgccgtcg ttgtagttat tgacgaagac ggaaccagcc ttcaggcggc ggctcatgcg
    16021 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
    16081 ggcaagctgt agcgcctggt cttctgatgt gaaacgcgtg accaccagca ccggaccgaa
    16141 aatctcttcg cggcttaagg acgcattcgg gtctacatcc acaaagatgg tcgggccgat
    16201 ggcggcagcc agctcagcgt tacgaccatc cagcagcagt tgccctttgc tttcgccttc
    16261 ccgaataaag ctatggaccg agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt
    16321 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
    16381 gaattcatcg gcgatgctct cttccagcaa caaacgcgtt ccggcgatgc acacctggcc
    16441 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
    16501 ggcgaaaacg aggttggcgc ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct
    16561 gtcgcccgca tctttcagca gctgtttccc ggtacgggtt gaaccggtaa aggcaatggc
    16621 gtcgatatcg ttatgacgcg acagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
    16681 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
    16741 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
    16801 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
    16861 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
    16921 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
    16981 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
    17041 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
    17101 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
    17161 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
    17221 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
    17281 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
    17341 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
    17401 ggccgtatgc ccgtttattt ctggcgacat tctttatata cacttcatcc ttcaagccgc
    17461 ctctgccttg gctgcgttcg ctcccccgaa tcacttacct gagtaagctc atcgggattc
    17521 gctcacttgc cgccttgatg caacttgaat gattttgtgt atagattgcc gggcgcattg
    17581 cgcttacccg gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa
    17641 ttcggcaaat acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat
    17701 aactttgccc cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt
    17761 ccagtactgt gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg
    17821 gctggtacgt ttcaaagatc atcgccagcg tgcgattcgg gttaccatta tgaaccagct
    17881 tcattgacac accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg
    17941 gctcatcagg tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca
    18001 gcagcttttg cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag
    18061 cactgtgagt cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc
    18121 ggatttccga caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt
    18181 aaactgtagt ggtcagagtc gctgtttttc agcgatatgg tgctgacaag cggtgataaa
    18241 gccctcgaac aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg
    18301 tacgcccagc gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga
    18361 gcgagcttca acacgcaacc gtgggctaac gaccttcgcc ccttgcccat gcagagagtt
    18421 tacccaaaag ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg
    18481 aacttcatgc gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgttc
    18541 cagcaattca ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag
    18601 ttcctgtaaa ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat
    18661 taacgccatg ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc
    18721 atataggtgc ggctgcacat tgctgggact accaggaaga taaatgccat cgagtttcgg
    18781 caaaagttgt tcaagtaatg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc
    18841 gcctgcgtgg atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgatcctt
    18901 aagcctgttc ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct
    18961 caatctcaca aagtggacta aattatcgcc attactgcgg tttaaatgac ctttatgttc
    19021 aatatttttt caatctagca gtgggttttc atttttgcaa actcaattta acatttgaca
    19081 aacatttagt ttgcatacag attcgaatgg tggtcattat attttacgct ttgataacga
    19141 gcggaaaaca aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt
    19201 tgttcagcag tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct
    19261 ggagcgctac ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt
    19321 ccgtggaaaa cgtatccctg tttcaagcct gaagaagttg gagaaagggt gttatttccc
    19381 ggcctcggtg tttgcaatgg atattctggg caacgtagta gaagaagcgg gtctgggtca
    19441 ggaaatgggc gagccggatc gtacttgtgt tcctgttctc ggttccttaa ccccttctgc
    19501 cgccgatcca gagtttatcg gtcagatgct cctgaccatg gtcgatgaag atggcgctcc
    19561 ctttgacgtt gagccgcgga acgttcttaa ccgcctgtgg cagcagctac gccagcgcgg
    19621 attgttcccg gttgtagcgg tagagctgga gttctattta ctggatcgtc agcgcgacgc
    19681 tgaagggtat ctgcaaccgc cctgcgcgcc cggcaccgat gaccgcaata cacaaagcca
    19741 ggtttactcc gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact
    19801 ggcgcagtta cagttaattc ccgccgacgg tgcggtcgcc gaagcctcgc cgggtcagtt
    19861 tgaaatcaac ctttaccata ccgataacgt gctggaagcc tgcgatgatg cgctggcgct
    19921 aaaacgcctg gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa
    19981 gccgtatgaa gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg
    20041 tggcgagaat gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct
    20101 cgccgggatg attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc
    20161 gtatcgccgc ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa
    20221 ccgcaccgtc gccctgcgta ttccgtgcgg cgaccgtcat aatcaccgcg tggaatatcg
    20281 cgtggcgggt gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt
    20341 gcatggcctt gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca
    20401 gtaaggctta cccttcccga ttcgccagag cgatgcccta ggtgagttta tcgagaatga
    20461 tcacctgcgc cgctatttag gcgaacggtt ttgccatgtg tatcacgcct gtaaaaatga
    20521 tgagctgttg cagtttgagc gcctcatcac agaaacagaa attgagtgga tgttgaaaaa
    20581 cgcgtaattc cccatgcggg gttgtttcat cggcctgagc ggagccaggc ctgacatttg
    20641 ctggtaatcc agacatttcc cggatgtcgc gttatggagc gcaggcggca acgggcttcc
    20701 gttaacgatt aagactgtga cgaggaaatg agatgatgcc gatgattaaa tccccgcatg
    20761 gcgagggagg ctgcgtatgc gcccctcctg ctactgactg gactccgccc cctttgcttc
    20821 ccttgctaaa caggtttgat ttcaggtcga cacgaccgca aaccttatta cgcaggggag
    20881 gcagcaatta tggctattaa ttcaccactg aatattgctg cgcaacccgg caaaacccgt
    20941 ctgcgaaaat cactgaaatt gtggcaggtg gtaatgatgg gtctggccta tctcacgccg
    21001 atgaccgtat ttgatacttt tggtattgtc tccggcatta gcgacggtca cgttccggcg
    21061 tcctatttgc tggcgctggc gggcgtgctg tttaccgcta tcagctacgg caaactggtt
    21121 cgccagtttc cggaggccgg ttcggcctat acctacgcgc aaaagtcgat taatccgcac
    21181 gtcggattta tggtcggctg gtcatcactg ctggattatc tctttttgcc aatgatcaac
    21241 gttttgttgg cgaaaatcta tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg
    21301 gtggtaacct tcgtcgccat tttaaccgcc gcgaatctga agagcgtcaa cctggtcgct
    21361 aacttcaata ccctgtttgt actggtgcaa atctccatca tggtggtgtt tatcttcctg
    21421 gtggttcagg gactgcataa aggagaaggc gttggcaccg tctggtcact tcagccgttt
    21481 atcagcgaga acgcgcacct gatcccgatt attaccggtg cgacgattgt ctgtttctcg
    21541 ttcctcggtt tcgatgcggt gaccacgctt tcggaagaga cgccggacgc cgcacgcgtg
    21601 atcccgaaag ccatcttcct gacggcggta tacggcggcg ttatctttat cgcggcgtcg
    21661 ttctttatgc agctgttctt tcccgatatc agccgcttta aagacccgga cgccgcactg
    21721 cctgaaattg cgctgtacgt cggcggcaag ctctttcagt cgattttcct ctgcaccacg
    21781 tttgtgaaca cattagcgtc tggcctggct tcacatgcca gcgtgtcgcg tctgctgtat
    21841 gtgatggggc gtgacaatgt gtttccggag cgcgtgtttg gctatgtgca cccaaaatgg
    21901 cggactccgg cactcaacgt cattatggtc gggattgtcg cgctgtcggc gctgttcttt
    21961 gatttagtca ccgcgacagc actgattaac ttcggtgctc tggtggcgtt taccttcgtg
    22021 aatctgtcgg tgtttaatca tttctggcgg cgtaaaggaa tgaataaaag ctggaaggat
    22081 cacttccact atttgctgat gccgctggtt ggcgcgctga cggtgggtgt gctgtggatt
    22141 aacctagagt caacgtcact gacactcggt ctggtatggg cttcgttagg cggcgcatat
    22201 ttgtggtatc tgatccgccg ctatcgcaaa gtgccgctgt acgaaggtga cagaacgccg
    22261 gtgagcgaaa cgtaatagcc ggatgcgcgg cgcattatcc tgcctgaggg taacgcgccg
    22321 cgccattaac tataaaaaga tgtatgaccc ggatcacgag tgctatcatc cgcccttatg
    22381 cttagcgcat taaccccagg ggcaaataat gaaccacgat atcccgctaa aatattttga
    22441 tattgccgat gagtacgcga ccgaatgcgc agagcctgtc gcagaggctg aacgcacacc
    22501 gctggcccac tacttccagt tactgctcac ccgtttgatg aacaatgaag agatcagcga
    22561 agaagcccaa catgaaatgg ctgctgaagc ggggattaat cccgtacgca ttgatgagat
    22621 cgcagagttt ttgaatcaat ggggtaatga gtaattattt aattgcagac gatcggtcag
    22681 tatcgttgtt cggataaagc gttcacgccg catccggcta acaatgacac gcactgcagc
    22741 gtgctttaat gggtttctca cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag
    22801 ttatagttat agagaaatca cgggtacgaa tgttgaacag atggcgacgc cacctgttca
    22861 acgaccaacc acacggccct cattacgacc cctgaacaca aacggtaatg gcaaatgtca
    22921 ttaaatatta gtaagatagc ctctttacat aaaatccctt aatattatgc gccaggtatt
    22981 atcgtctctt ttggtgattg ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc
    23041 ccccccgcat gctgatatcc gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa
    23101 cacctttaac ccatccaaag cgagcagtgg gttaattgtc gatacccttg ccgcccagtt
    23161 ttatgatcga ctgctggatg tcgatcccta tacctatcgc ctgatgccgg aacttgccga
    23221 aagctgggaa gtactcgaca acggcgcgac ctatcgcttc cacctgcgtc gcgatgttcc
    23281 gtttcaaaaa accgactggt ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt
    23341 taccttccag cgaatttttg accgcaacaa cccgtggcat aacgtcaacg gcagcaactt
    23401 cccctacttc gacagcctgc aatttgccga taacgtgaaa agcgtccgca aactggataa
    23461 tcataccgtt gagttccgac tcgcccagcc ggatgcttct tttttgtggc acctcgcaac
    23521 ccattatgct tcggtcatgt cggcagaata tgcccggaag ttagagaaag aagatcgcca
    23581 ggagcaactc gaccgtcaac cggtcggcac cggtccgtat cagttgtcgg aataccgcgc
    23641 cgggcaattt attcgcctac aacgtcatga tgacttctgg cgcggtaaac cgttaatgcc
    23701 gcaggtagtg gtggatttag gctccggcgg caccggacgt ctgtcgaaac tcctgaccgg
    23761 ggaatgcgac gttctggcct ggcctgctgc cagccagcta tccattttgc gtgacgaccc
    23821 gcgcttgcgt ttaacgctgc gtcctgggat gaacgtcgcc tatctggcat ttaacaccgc
    23881 caaaccgccg ctaaataatc ccgctgtccg ccatgcactg gcactggcga ttaataacca
    23941 gcgcctgatg caatccatct attatggtac ggctgaaacg gcggcctcta ttttaccgcg
    24001 cgcctcgtgg gcctatgaca acgaggctaa aattactgaa tacaatccgg cgaaatcgcg
    24061 cgaacagttg aaggcgttgg ggctggaaaa cttaacgctg aaactgtggg tgcccacacg
    24121 ttcgcaggcg tggaacccca gtccactgaa aactgccgaa ctgattcagg cggatatggc
    24181 gcaggttggc gtaaaagtgg tgattgtgcc ggtagaaggt cgctttcagg aggcgcggtt
    24241 gatggatatg agccatgatc tgacgttatc cggttgggcg acggacagta acgacccgga
    24301 cagtttcttc cgtcctttac tgagctgcgc ggcaattcat tcacagacca acctcgccca
    24361 ctggtgcgat ccgaaattcg acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc
    24421 ggcgcgtatt gaagcctatg acgaagcgca gagtattctg gcgcaggaat tgcccatttt
    24481 gccgctggcg tcgtcattgc gtttgcaggc ctatcggtac gatatcaaag gtctggtact
    24541 tagcccgttt ggtaacgcct cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa
    24601 aaaaccatga ttatcttcac cttacgccgc attttgttat tgattgtcac cttgttcctg
    24661 ctgaccttcg ttggcttcag cttgagctat ttcacccctc atgcgccgtt acaaggcgcg
    24721 tcactgtgga atgcctgggt gttctggttt aacggcctga tccactggga ttttggtgtt
    24781 tccagcatta acggtcaacc gattgccgaa cagttaaaag aggtcttccc ggcgacgatg
    24841 gaactgtgca tcctcgcctt cggttttgcg ctgattgttg gcatccccgt ggggatgatt
    24901 gccgggatta cgcgccataa gtggcaggat aacttgatca acgccatcgc cttactgggc
    24961 ttttcaatcc cggtgttctg gctggcgctt ctgttgacgc tgttttgttc actcacgcta
    25021 ggttggttgc ccgtttcagg gcgtttcgat ctgctctacg aagtgaaacc gattaccggt
    25081 tttgcgttga ttgatgcctg gctttcggac tcaccatggc gggatgaaat gatcatgagc
    25141 gcaatccgcc atatgatatt gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg
    25201 atccgcctga tgcgtatcag cactatcgaa gtgtatgacc agaactatgt aaaagcggcg
    25261 gcgacccgcg gtttgtcgcg ctttactatt ttgcgtcgcc acgttctgca taacgcgtta
    25321 ccgccggtta ttcctcgcct gggcttacag ttttccacca tgctgacgct ggcgatgatc
    25381 accgaaatgg tttttagctg gccaggttta gggcgctggt taattaacgc catccgccag
    25441 caggattacg cagccatttc cgccggagtg atggtgtgtg gctcactggt cattattgtt
    25501 aacgtgattt ctgatatttt gggtgccatg gctaaccctc tgaaacataa ggaatggtat
    25561 gccttacgat agcgtataca gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg
    25621 caaattttat agtgacgcct ctgcgatggt cggcctgtac ggctgcgcgg ggctggctgt
    25681 actgtgtatt tttggcggct ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta
    25741 tcaattattg ccgccgtcat ggtcgcgcta tggcgaagtt tctttcttcc tggggactga
    25801 cgacctcgga cgcgatgttt taagccgttt actgagcgga gctgcgccaa ccgtgggtgg
    25861 cgcatttgtg gtgacgcttg ccgcgacgat ctgtggcctg gtactgggca ccttcgccgg
    25921 ggcgacacac ggcctccgct cagcagtgct taaccatatt ctggatacct tactggcgat
    25981 cccttcgctg ctgttggcca ttatcgttgt agcgtttgcc ggaccgagtt tgtcccacgc
    26041 tatgtttgct gtctggctgg cattgctgcc gcgtatggta cgttcgattt acagcatggt
    26101 gcatgacgaa ctggaaaaag agtacgttat cgccgcccgt ctggatggcg catcaacgct
    26161 gaatattctc tggtttgctg tgatgccaaa catcaccgcc gggctggtca ccgagatcac
    26221 ccgcgcactg tcgatggcaa ttctcgatat cgccgcgctg ggctttctcg atctcggcgc
    26281 acaactcccc tcgcctgaat ggggagcgat gctcggtgat gcgctggaac tgatttatgt
    26341 cgccccgtgg actgtcatgc tgccaggtgc ggcaattatg attagcgtgt tgttggttaa
    26401 cctccttggc gatggcgttc gtcgtgcgat tattgcgggg gtggaataat gccattactc
    26461 gatattcgta acctgaccat tgaatttaaa accggtgatg agtgggttaa agccgtcgac
    26521 cgggtaagta tgaccttaac cgaaggtgaa atccgcggtc ttgttggtga atccggttcc
    26581 ggcaagagtt tgattgcgaa agcgatttgt ggggtgaata aagataactg gcgtgttact
    26641 gctgaccgta tgcgttttga tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc
    26701 aaactggttg gccataacgt gtcgatgatt ttccaggaac cgcagtcgtg tcttgaccct
    26761 tcagaacgtg tgggtcgcca gttgatgcaa aacatcccag cctggaccta taaaggccgt
    26821 tggtggcagc gttttggctg gcgcaaacgc cgtgccattg aactgctgca ccgcgtgggg
    26881 attaaagatc acaaagatgc gatgcgcagt tttccctatg agttgaccga aggtgaatgt
    26941 cagaaagtga tgatagccat tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa
    27001 ccgaccaact caatggagcc aacaacccag gcgcaaatct ttcgcctgct gacgcgtctc
    27061 aaccaaaaca gcaataccac tattttgctt atcagccatg acttacaaat gcttagccaa
    27121 tgggcggata aaattaacgt gctttactgc ggtcaaacag tggaaaccgc gccgagtaag
    27181 gagttggtga cgatgccaca tcatccttat acccaggcgc tgatccgcgc gataccagac
    27241 ttcggcagcg cgatgccgca taaaagtcgc ctcaatacgc tgcccggcgc tatcccactg
    27301 ctggaacagt taccgattgg ttgtcgtctg gggccacgtt gcccgtatgc acaacgagaa
    27361 tgcattgtga cgccacgttt gacgggggcg aaaaatcatc tctatgcctg tcatttcccg
    27421 ctgaacatgg agaaagagtg agatgatcga aacgctgctt gaagtgcgta atttaagtaa
    27481 aacgttccgc taccggaccg gctggtttcg tcgtcagacc gtagaagcgg taaaaccctt
    27541 gagctttacg ctacgtgaag gccagacact ggcgattatt ggcgagaatg gttcgggtaa
    27601 atccacgctg gcaaaaatgc tggcgggaat gatagagcct accagcggcg agttattgat
    27661 tgacgatcat ccactgcatt ttggcgatta ttccttccgc agtcagcgta ttcgcatgat
    27721 ttttcaggat ccttcgacct cgttgaatcc ccgtcaacgt atctcgcaaa ttctggattt
    27781 tccactgcgc ctgaacaccg acctggaacc ggaacagcgt cgcaaacaga ttattgaaac
    27841 gatgcgtatg gttgggctgc taccggatca cgtcagttac tatccgcata tgttggcacc
    27901 cggacaaaaa cagcgtctgg gtctggcgcg cgcgctgata ttgcgcccaa aagtcattat
    27961 tgccgatgaa gccctcgctt cactggatat gtcgatgcgt tcgcagttga ttaatctgat
    28021 gctggaatta caggaaaaac agggcatttc gtatatttat gttacccagc atatcggaat
    28081 gatgaagcac attagcgacc aggtgctggt gatgcatcag ggcgaggttg tcgagcgagg
    28141 cagcaccgcg gatgtgctgg catcgccgct gcatgaactc accaaacggc tgattgccgg
    28201 gcactttggc gaagcattga cagcggatgc gtggcgtaaa gatcgctaat tttctgaatc
    28261 ctccttccag attatccacc tcattctgac aagagattaa atcttgtcag aatgaggtga
    28321 tggataaaat aaaatcaaat gcgcgtgatt taagacgcaa tctcactttg caggaacgaa
    28381 agctctggcg atatcttcgc agccgacgtt ttggtgattt caaatttcgc cgtcaacatc
    28441 cggtggggag ctacattctc gattttgctt gctgctcggc gcgcgtactc gttgagctgg
    28501 atggtgggca gcatgatttg gcagttgcct atgataccag gcgcactagc tggcttgagt
    28561 cgcagagctg gaccgtgctg cgtttctggc ataacgagat tgattgtaat gaggaggcgg
    28621 tgctggagat tattctgcag gaactgaacc gccggtcacc ctcaccctga aagggcgagg
    28681 gggccgaccg agccgaatag ctgttgtggt gaaaacatgg agacagtgct ggagaatatt
    28741 cggcaaggtc tgaaccatcc cagccatcgc cataaaaggg ttaggggctg tatgagcctg
    28801 tttgttgctg gggtaacaat atttgcacaa tatggtcccc tcgcccctct ggggagaggg
    28861 ttagggtaag gggaaaagcg cccaccctgc cgcagcctgc tccggtcgga cctggcaact
    28921 atagctactc acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa
    28981 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
    29041 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact atccatcgcc
    29101 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
    29161 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg
    29221 cagtgcgatg ttgcagaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt
    29281 tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
    29341 ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa ttgcccacga catcagctcc
    29401 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
    29461 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
    29521 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
    29581 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcctc cggtatcaaa
    29641 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
    29701 attgaagatg tgggtaactc cgcggcattc ctgtgctccg atctctctgc cggtatctcc
    29761 ggtgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga actcgaattg
    29821 aaataatcat tctgttggta aatattgggc ggcgttctgc cgcccgttat ctctgttata
    29881 cctttctgat atttgttatc gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat
    29941 aacgatttcc tttacgacca aggagcgccc atggaacaac gccacatcac cggcaaaagc
    30001 cactggtatc atgaaacgca atccagtact gcggagtatg acgttctgcc tctggtcccg
    30061 gaagccgcaa aggtcagcga tccctttctg ctctacgtga tccttgatga agaaacgctg
    30121 gctcccttcc tttcatggct ggtccctgcg cgcgttcttg cagtggaatt gttccctgac
    30181 cagcttaccg tgacccgttc acagaccttc accgcttatg aacgcttgtc tacggccctg
    30241 acggttgctc aggtttgcgg cgtccagcgg ttatgtaact actattcggc gcgacttacg
    30301 ccgctccccg ggcctgattc ctccagggaa agcaatcata ggttggcaca aatcacgcaa
    30361 tatgcccgcc aactggctag ctcgccttct attatcgaca accgatcgcg ccagcatctg
    30421 aatgacgtcg gtcttactgc cggggactgt gtgatcatta accaaatcat tggttttatt
    30481 ggctttcagg cgcggaccat tgcgacattt caggcttatc tcgggcaccc ggtacgctgg
    30541 ttacccggtc tggaaataca aaactacgcc gacgcgtcac tgtttgctga tgaatcaata
    30601 cgctggcgaa gcagctatga ggtggaaaaa ctacctgaag agtacacaaa aagttcaaca
    30661 gcagaacttt gccaactggc cgaaacacac tctctccacc ctatttcact ttcccttctc
    30721 gaaaggttgt taaacagtac acgggttaat acacagccgg ataataagct tgcggcgttg
    30781 ttatgcgcgc ggataaatgg cagtcctgct tgttttgccg cctgtatgga ttcatcaaat
    30841 gaatataaaa aaatcagacc ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct
    30901 gaccgtcatt ctgttgagca cgctaccgtt caggcgatac aatggctgac ccgagcaccc
    30961 gatcgcttta gcgccgccca gttcagccct ttactcgaac acgaaaaatc atcaacgcag
    31021 attattaatc tgctggtatg gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg
    31081 ttgggtgaga catattaacc ttgccgcgtc agacagattc gcgtaaaact gtcagccgct
    31141 ctaatggcca ccaaaataga caattatgtt tcaggacaac ccgctgctag cgcagcttaa
    31201 acagcaactg cattcccaga cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg
    31261 ctttggcttc ctggaagtcg acgcgcaaaa aagttatttc attccgccgc cgcagatgaa
    31321 aaaagtcatg catggcgacc gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc
    31381 cgcagagcca gaagaactgg ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg
    31441 caaaaatgac cgtctggcca tcgttcctga tcatccactc ttaaaagacg ccattccttg
    31501 ccgcgcagcc cgtggcctga accacgagtt taaagaaggc gactgggcgg ttgccgaaat
    31561 gcgccgtcat ccgctgaaag gcgatcgttc tttctatgct gaactgacac aatacatcac
    31621 ttttggtgac gaccactttg taccgtggtg ggttaccctt gcgcgccata atctggaaaa
    31681 agaagcacca gacggcgtcg ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct
    31741 gaccgcgctg gattttgtca ccatcgacag tgccagcaca gaagatatgg atgacgccct
    31801 tttcgctaag gcgttgccgg atgacaaact tcagctgatt gtggcgattg ccgatccaac
    31861 cgcgtggatt gctgaaggca gcaagctgga caaagccgcg aaaattcgcg cattcaccaa
    31921 ctatctgcct ggcttcaaca tccctatgct gcctcgcgag ctttctgacg atctctgctc
    31981 actgcgcgcc aatgaagtcc gcccggtact ggcatgccgc ataacgctct ccgctgatgg
    32041 caccattgaa gataatatcg aattctttgc cgccaccatc gaatccaaag caaagctggt
    32101 gtatgaccag gtttctgact ggctggaaaa taccggtgac tggcagcctg aaagtgaagc
    32161 aattgccgaa caagtccgtt tgctagcgca aatttgccaa cgccgcggcg agtggcgtca
    32221 taaccacgca ctggtgttta aagatcgccc ggattaccgc tttattctcg gtgaaaaagg
    32281 tgaagtgctg gatatcgtcg ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc
    32341 gatgattgcc gctaacattt gtgcggcccg cgtactgcgc gataagctcg gttttggcat
    32401 ctataacgtg catatgggct ttgatccggc gaatgccgac gcgctggcag cgttgctgaa
    32461 aacgcacggt ctgcatgtcg atgccgaaga agtgctcacg ctggacggtt tctgcaaact
    32521 gcgtcgtgaa ctggacgcgc aaccaactgg tttcctcgac agccgcattc gtcgcttcca
    32581 gtcatttgct gaaattagca ctgaacccgg tcctcacttt ggcctcggtc tggaagcata
    32641 cgccacctgg acttcgccga tccgtaaata tggcgacatg atcaaccacc gtctgctgaa
    32701 agcggttatc aaaggcgaaa ctgcgacgcg cccacaggat gaaatcactg tccaaatggc
    32761 cgagcgtcgc cgtctcaacc ggatggcaga acgtgatgtt ggtgactggt tatacgcacg
    32821 cttcctgaaa gacaaagccg ggaccgacac ccgtttcgca gcggaaattg tcgatatcag
    32881 ccgtggcggc atgcgtgttc gtttggttga taacggcgct atcgccttta ttccggcacc
    32941 tttcttacac gctgtgcgcg atgaactggt ttgcagccag gaaaacggca ctgtacaaat
    33001 taaaggtgaa acggtttaca aagtgactga cgttattgac gtcactattg ccgaagtccg
    33061 catggaaacc cgcagcatta ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc
    33121 ctcatggatg ggccgtttat ttccccgctc taccttcgtt atatccaggc agatttttta
    33181 actgactttc gtttgaaaac tggcgttttt ccaaaactgg attacaaata aatatataaa
    33241 aaaaacaatg cgtttagccg ccgttaattt catgtaagtc atgttgttcc ttttatttaa
    33301 ccgcggactc cgcagttaac cggaggatat gcatcatgaa aaccgttagg gagtccacaa
    33361 cgttgtacaa ctttctcgga tcgcacaatc catactggcg gttgacggaa agcagcgatg
    33421 ttttgcgctt ttctaccacc gaaaccacag aacctgatcg tactttgcag ttatctgccg
    33481 aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg atgagtctga
    33541 ccgtcgatga gagcgatctt tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat
    33601 gggctggcaa cgcgtctgca tggcatgaca caccggcggt tgctcgtgat ttatcacacg
    33661 ggctttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtc attctcgaca
    33721 gccgggggaa tattcaacgc ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac
    33781 acgacgtcat tgggcaaagc gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat
    33841 ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga
    33901 taccaacatg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc cacagcggca
    33961 gtggcaaaaa cgagattttt ttaatctgtt ccggcaccga cattaccgaa gagcgccgcg
    34021 ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg ccgaatcgta
    34081 acgcaatgca ggatttaatc gatcacgcta ttaatcatgc agataacaat aaagttgggg
    34141 ttgtgtatct tgatttggat aatttcaaaa aggtcaacga cgcctatggg catttgtttg
    34201 gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa catgaccagg
    34261 tgttggcgcg tccaggtggg gatgagtttc tggtactggc atccaacacc tcacaaagcg
    34321 cgctggaagc aatggcatca cgaattttga cccgcttacg gctccccttt cgcattggtt
    34381 taattgaagt ttataccagc tgttcagtag gtattgcact ctctcccgaa catggttcag
    34441 acagcacggc tattattcgt cacgccgaca cagcaatgta cacagcgaag gaaggcggac
    34501 gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa tatctctggc
    34561 tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac tatcaaccga
    34621 aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg cagtcacctg
    34681 aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca gggctaattg
    34741 tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag tggcgggata
    34801 aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc gatcaaacca
    34861 ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc cctatagatg
    34921 ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct gttattcaac
    34981 aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc tactcttcac
    35041 tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag
    35101 atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg
    35161 cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat gcttttttaa
    35221 ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg
    35281 ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa tatatatact
    35341 tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc gttgctgtta
    35401 aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg ctataaggat
    35461 gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata ttgtgttaga
    35521 tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac ttaaagcaga
    35581 actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt gggagattta
    35641 acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta tttttttatt
    35701 tagtattata acgttataag aattacagcg atgaattccc gacaacaaac tattctacag
    35761 atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac tggagtttct
    35821 gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca
    35881 catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat gagcaactat
    35941 acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc
    36001 tttatcgaaa atggtagcag caatgccctg ctcgcccgga ctctgggcga acagaagaaa
    36061 aatgtcacta tcatcacagt cagcagctac attgcgcatt tgctgaaaga cgcgccttgt
    36121 gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg
    36181 acacgccagt gcatccagca ggtacatttc agcaaagcat ttattggtat tgatggctgg
    36241 caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt caatgccgtg
    36301 ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg tgctgtacat
    36361 tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc
    36421 gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa
    36481 ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct aagatgattc
    36541 ctggttgata attaagacta tttacctgtt attaacactg tcaagatata aaattattat
    36601 cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg cgagcagatt
    36661 tcacggaata atttcaccag gcttattctt agctattata gttatagaga gcttacttcc
    36721 gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc
    36781 tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt ctaaacggga
    36841 ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg
    36901 cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc accaggtcgg
    36961 taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct tgattctgtt
    37021 cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg gatatatccg
    37081 tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt caagtagtga
    37141 ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa ctgctccgcc
    37201 gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat catcgagatc
    37261 gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac gctgaatacg
    37321 caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa tacgtccggt
    37381 ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg ctgtaaagag
    37441 gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc
    37501 accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg gcgctggtca
    37561 ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc ctgaccgaat
    37621 acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag gccacatttt
    37681 tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat gccaagatcg
    37741 accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag cggtgcatct
    37801 ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc accagaggca
    37861 tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg cgctgcagtg
    37921 ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg ttgaagttcg
    37981 cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag acgacaatcg
    38041 cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata atcaagggca
    38101 acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa cgtcatgacc
    38161 agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc gagcattcta
    38221 cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt ttctgctccg
    38281 tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa gtcggatttt
    38341 tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca ggcccgacaa
    38401 gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca gcgataacga
    38461 ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct ctctttggca
    38521 cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac acgcatggtc
    38581 ggatgacgct gaagctggcg cgtaatatag atctgtgcag cctcactacc gtcgcgcgct
    38641 tcgatgatat ccgcaagcat caattcagca tcggcaccgg tgttctcttc caccgcgcgt
    38701 tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt ttgcaacatt
    38761 tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa actttcgacg
    38821 gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga tacgcgggcg
    38881 ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc gagatcgtcg
    38941 ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac gcgctgttta
    39001 tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca ctcactggta
    39061 gcctggtaga tttgtagcaa ctgttgcaga gcgccaatgc ggaagtcagt ttcatcggtc
    39121 agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta atcacgccct
    39181 agttgttgaa tcgccaacag acgctgttca taggtcagcg aggcgctttc cattagggtc
    39241 tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt tccgagcgta
    39301 aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag gtctaccgct
    39361 ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg cgacaagcgg
    39421 ttggcttcat cttgcttatt ttgttgcgca cttctgcggc ccatatacca gccataggca
    39481 gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc cttcgccgct
    39541 gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc cagtcgcttt
    39601 attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag gccacaaatc
    39661 aaccaaccga tagcaaaccc cgcagcaaac aataccgcca gcaatgtgga aatacggtac
    39721 tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc acccaacgtg
    39781 accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt cacattactt
    39841 cccgttatgt ggttagagcg aataaattat ggtcaactta ccgcaatagc cctttaaagt
    39901 accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat ttaagggcta
    39961 aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt cgcgattttc
    40021 ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg ccaccgccac
    40081 cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca tcccgagcag
    40141 caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg ttaacgttcg
    40201 ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa acatcgtgtg
    40261 accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat attgtgggat
    40321 atttttctct tcagccaact gttctttcac taaatttccg cgttctgctc gctttaaagt
    40381 gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa aaggtcgtgg
    40441 ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaagga ttgcggccgc
    40501 cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc agagaaacca
    40561 gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt cagtaaccca
    40621 aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc cagaaatcca
    40681 tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc tggcaatcga
    40741 acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc cagttccagg
    40801 aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt aggtgaaccc
    40861 cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga atttcatgca
    40921 gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc tgatggtggg
    40981 atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg atatttccgg
    41041 gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg ccctgttcag
    41101 cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg ccgaggaagg
    41161 ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc tgaataaaat
    41221 ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc accagttagg
    41281 cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac tccttggcgt
    41341 caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga ccgaagcagg
    41401 gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata acgaacatta
    41461 tctcgatact aaagccgaga aaatgggcca tttgctgaac aaataaccct cttgcattgt
    41521 gtaattagtt tgcttgccgg aagcaaaata accggcaagc aaatacttgt tacttcaaca
    41581 tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac tccgtcgcgg
    41641 tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc gtaagcgtca
    41701 ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc ttctcttccc
    41761 cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc aggatgccca
    41821 tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc acacgaattc
    41881 caagcagacg aggacctttt gccgcccagt cacggcttga gcctgatcca tactctttcc
    41941 cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca tcataaatag
    42001 agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca ggcaccattt
    42061 cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg ttaccacgcc
    42121 gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga tatcgacccg
    42181 ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg actgaatccc
    42241 ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt gttgcctgca
    42301 tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa ccgtaggtat
    42361 cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact tctgcgtact
    42421 ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct tgtgccgatg
    42481 gccagatatc tttcagataa accgggtcgc ctttacgatc atgaccaata ggctcagcag
    42541 ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc ggcgaggcca
    42601 gccagttagt tttaaccagc ggatggatac ggccttcaaa gttacggttg ccggacagca
    42661 ccgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg ggcagcggcc
    42721 cagagttacc aatacaggtg gtacaaccgt atcccacaag gttaaacccc agttcgtcga
    42781 gatacggtgt cagtttcgct tttgccagat aatcagaaac gactttcgaa cccggtgcca
    42841 gcgacgcttt gacccacggt tgccgcttga ggcccagagt tacggctttt ttcgccagca
    42901 agcctgcggc catcagcaca cttgggttag aggtgttggt gcacgaggtt atcgcagcaa
    42961 tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa tcgaccggct
    43021 ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat gcttttggta
    43081 catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc gcttcaacgt
    43141 cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg ttacgccaca
    43201 tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg cgcccgctta
    43261 aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg gcaccatatt
    43321 ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc agaccatcac
    43381 cataaaattc gacgaatttc cccaccacgc catgtttgcg cagcatttgg gtaacagtga
    43441 gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta aagcccacta
    43501 cgtccgggat aagcatggaa accggctggc c
//
LOCUS       DAHBHC010000038        43461 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHBHC010000038 DAHBHC010000000
VERSION     DAHBHC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.038"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..202
                     /locus_tag="M2A35_002484"
     CDS             <1..202
                     /locus_tag="M2A35_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198773.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
     gene            complement(199..447)
                     /locus_tag="M2A35_002485"
     CDS             complement(199..447)
                     /locus_tag="M2A35_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198774.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            1638..2792
                     /locus_tag="M2A35_002486"
     CDS             1638..2792
                     /locus_tag="M2A35_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCB7198775.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2943..3767
                     /locus_tag="M2A35_002487"
     CDS             2943..3767
                     /locus_tag="M2A35_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCB7198776.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3852..5054
                     /locus_tag="M2A35_002488"
     CDS             3852..5054
                     /locus_tag="M2A35_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCB7198777.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            5114..5698
                     /locus_tag="M2A35_002489"
     CDS             5114..5698
                     /locus_tag="M2A35_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCB7198778.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            6093..6551
                     /gene="traH"
                     /locus_tag="M2A35_002490"
     CDS             6093..6551
                     /gene="traH"
                     /locus_tag="M2A35_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HCB7198779.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            6548..7366
                     /gene="traI"
                     /locus_tag="M2A35_002491"
     CDS             6548..7366
                     /gene="traI"
                     /locus_tag="M2A35_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HCB7198780.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            7363..8511
                     /gene="traJ"
                     /locus_tag="M2A35_002492"
     CDS             7363..8511
                     /gene="traJ"
                     /locus_tag="M2A35_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCB7198781.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKCRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            8508..8798
                     /locus_tag="M2A35_002493"
     CDS             8508..8798
                     /locus_tag="M2A35_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCB7198782.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8813..9364
                     /locus_tag="M2A35_002494"
     CDS             8813..9364
                     /locus_tag="M2A35_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HCB7198783.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            9453..13220
                     /locus_tag="M2A35_002495"
     CDS             9453..13220
                     /locus_tag="M2A35_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCB7198784.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            13238..13585
                     /locus_tag="M2A35_002496"
     CDS             13238..13585
                     /locus_tag="M2A35_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCB7198785.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            13582..14274
                     /locus_tag="M2A35_002497"
     CDS             13582..14274
                     /locus_tag="M2A35_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCB7198786.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            14285..15268
                     /gene="traN"
                     /locus_tag="M2A35_002498"
     CDS             14285..15268
                     /gene="traN"
                     /locus_tag="M2A35_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HCB7198787.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            15271..16560
                     /gene="traO"
                     /locus_tag="M2A35_002499"
     CDS             15271..16560
                     /gene="traO"
                     /locus_tag="M2A35_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCB7198788.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            16560..17264
                     /gene="traP"
                     /locus_tag="M2A35_002500"
     CDS             16560..17264
                     /gene="traP"
                     /locus_tag="M2A35_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HCB7198789.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            17264..17791
                     /gene="traQ"
                     /locus_tag="M2A35_002501"
     CDS             17264..17791
                     /gene="traQ"
                     /locus_tag="M2A35_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCB7198790.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17842..18246
                     /gene="traR"
                     /locus_tag="M2A35_002502"
     CDS             17842..18246
                     /gene="traR"
                     /locus_tag="M2A35_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HCB7198791.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVIAFLGGLIGLKKVGKQGGMGLAGC
                     ILSIVIGALLAAGPEMMSRSQKQLGISTISIG"
     gene            18310..18498
                     /gene="traS"
                     /locus_tag="M2A35_002503"
     CDS             18310..18498
                     /gene="traS"
                     /locus_tag="M2A35_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HCB7198792.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            18482..19282
                     /gene="traT"
                     /locus_tag="M2A35_002504"
     CDS             18482..19282
                     /gene="traT"
                     /locus_tag="M2A35_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HCB7198793.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            19372..22416
                     /gene="traU"
                     /locus_tag="M2A35_002505"
     CDS             19372..22416
                     /gene="traU"
                     /locus_tag="M2A35_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCB7198794.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMALAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAIEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            22416..23030
                     /gene="traV"
                     /locus_tag="M2A35_002506"
     CDS             22416..23030
                     /gene="traV"
                     /locus_tag="M2A35_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCB7198795.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22997..24199
                     /gene="traW"
                     /locus_tag="M2A35_002507"
     CDS             22997..24199
                     /gene="traW"
                     /locus_tag="M2A35_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCB7198796.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            24228..24812
                     /gene="traX"
                     /locus_tag="M2A35_002508"
     CDS             24228..24812
                     /gene="traX"
                     /locus_tag="M2A35_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCB7198797.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24909..27077
                     /locus_tag="M2A35_002509"
     CDS             24909..27077
                     /locus_tag="M2A35_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCB7198798.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGILPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            27151..27801
                     /gene="excA"
                     /locus_tag="M2A35_002510"
     CDS             27151..27801
                     /gene="excA"
                     /locus_tag="M2A35_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCB7198799.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(27873..28082)
                     /locus_tag="M2A35_002511"
     CDS             complement(27873..28082)
                     /locus_tag="M2A35_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCB7198800.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            28474..28650
                     /locus_tag="M2A35_002512"
     CDS             28474..28650
                     /locus_tag="M2A35_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198801.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            29309..29560
                     /locus_tag="M2A35_002513"
     CDS             29309..29560
                     /locus_tag="M2A35_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198802.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(29632..29784)
                     /locus_tag="M2A35_002514"
     CDS             complement(29632..29784)
                     /locus_tag="M2A35_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCB7198803.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            30076..31284
                     /gene="trbA"
                     /locus_tag="M2A35_002515"
     CDS             30076..31284
                     /gene="trbA"
                     /locus_tag="M2A35_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCB7198804.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            31303..32373
                     /gene="trbB"
                     /locus_tag="M2A35_002516"
     CDS             31303..32373
                     /gene="trbB"
                     /locus_tag="M2A35_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCB7198805.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            32366..34657
                     /locus_tag="M2A35_002517"
     CDS             32366..34657
                     /locus_tag="M2A35_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCB7198806.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(34694..37393)
                     /gene="nikB"
                     /locus_tag="M2A35_002518"
     CDS             complement(34694..37393)
                     /gene="nikB"
                     /locus_tag="M2A35_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HCB7198807.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG"
     gene            complement(37404..37736)
                     /gene="nikA"
                     /locus_tag="M2A35_002519"
     CDS             complement(37404..37736)
                     /gene="nikA"
                     /locus_tag="M2A35_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HCB7198808.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            37970..38305
                     /locus_tag="M2A35_002520"
     CDS             37970..38305
                     /locus_tag="M2A35_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCB7198809.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            complement(38390..39238)
                     /locus_tag="M2A35_002521"
     CDS             complement(38390..39238)
                     /locus_tag="M2A35_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCB7198810.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREAQDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            39587..39784
                     /locus_tag="M2A35_002522"
     CDS             39587..39784
                     /locus_tag="M2A35_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198811.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            39818..40069
                     /locus_tag="M2A35_002523"
     CDS             39818..40069
                     /locus_tag="M2A35_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7198812.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(40100..40270)
                     /locus_tag="M2A35_002524"
     CDS             complement(40100..40270)
                     /locus_tag="M2A35_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198813.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(40332..41273)
                     /locus_tag="M2A35_002525"
     CDS             complement(40332..41273)
                     /locus_tag="M2A35_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCB7198814.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            complement(41338..41604)
                     /locus_tag="M2A35_002526"
     CDS             complement(41338..41604)
                     /locus_tag="M2A35_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198815.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(41696..42130)
                     /locus_tag="M2A35_002527"
     CDS             complement(41696..42130)
                     /locus_tag="M2A35_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCB7198816.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(42859..43359)
                     /locus_tag="M2A35_002528"
     CDS             complement(42859..43359)
                     /locus_tag="M2A35_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCB7198817.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT        11477 a        11213 c        10599 g        10172 t
ORIGIN      
        1 gacaataggt ggaaaactca aagttactca gctttccacc acaggttatc tggggcaatt
       61 cgacttctgt gccattgcca gaatgggcaa cgcagaggat gcccactact gccaggtagt
      121 tgagagccca gcaggttcac gcaaatggta caaatacgag cataagacag gttgtattgc
      181 gtcgtgtgta acgctcaatt aagtttgata tccaaatact gaaaataccc cggcaccaca
      241 tgatgtattt tctgttggat aggatgttat ctgataggaa gtacctgcag gtacagcaaa
      301 gctgataaag gctgtttttc catatgcagg gttattgctg gcattcacgc taattagcgt
      361 cccaccaaca tatccctgca gtcgggatgt atttgcacat gcacctccgg cagatcctcc
      421 attacctcca gatgcataaa taaacaatgt actgccccct gaattccgcc ctgagaatga
      481 acctctatgt gaacctaagt ttgtgtaaga accattgagc gaaccagaag tcttccacgt
      541 accggattgg cactggccta gtgccaatcc ggtcggtggt cagggggtaa taaaattaat
      601 tactcagcat gtaattggta caaatcatcc gtagcaatga atcactttat cggcggtaag
      661 agtggtggtt cgatctatta caagccgatt caatgtccga ctggatacat tatgactgga
      721 acacgcatgt acggtattgg tgatggtgtt gatgaggaac atgtcgatgc ctactgttgc
      781 ccctttaatt aagctccagc accaggaaga ttgaggcatg tgactgatat ggtcccacca
      841 gactcataat ttccccaagc ataattactc acataccaaa cgttgttttg gtcggaatat
      901 acctgttgaa aaccaccaaa gggtgagcca ttcaggtaag tccatgagca atatgcatgg
      961 accccgagcc ttaaattacg agtgttttta gctatgttgt aggtctgcgt tgtgaattga
     1021 attttaggag cgccccaggt accggattgg cactggccta cgtgccaatc cggtacctgg
     1081 aaatcatcat cagcgtcgat atggacgaat ataaaaacat ttacgttata tcccaaaaac
     1141 acacaggtct tagggaggtt taaactgtgt attaacacct atcgtattga cgggcgtgaa
     1201 atggcagaaa cagaagtcgt tcctattgac atgcctgact ctaacggcga gatgacttgg
     1261 caagccaaaa actacacaca gtattcgtcc tactttatga agattacttg cttgaaatag
     1321 cttattggca aatggcatat acagttattg aaccattctg atcttcggtt gtatcgcacg
     1381 cagccgacca tgagttattg gctgatggga atgacctggt aagccatatg taaccagttc
     1441 gtgaggtgca tattccgccg ccacctgtaa cacgttttcc agttggacaa gtagctgttg
     1501 ctccaggaaa gcgccaacct gtggcctgtg ctgtagcaat ttgaagctca ccagatccta
     1561 cctttctcca ggtaccggat tggcacgctt gcagagaatg attgttggca ttattttttg
     1621 cctcttttga ggttagcatg ccgtctccac gcatccgtaa aatgtccctg tcacgcgcac
     1681 tggataagta cctgaaaaca gtttctgttc acaagaaagg gcatcaacag gagttttacc
     1741 ggagcaatgt tatcaagcga tatcccattg cccttcggaa tatggacgaa ataacaaccg
     1801 ttgatattgc tacatacaga gacgttcgtt tagcagaaat caacccccga acgggtaaac
     1861 ccattacagg taatactgta cgtcttgaac tcgcccttct gtcatctctg ttcaatattg
     1921 ctcgtgttga atggggaacc tgtcgtacta acccggttga actggttcgc aagccgaaag
     1981 tatcctcggg acgagatcgc cggctaacgt cttcagaaga acgtcgcctt tctcgctatt
     2041 tccgcgaaaa aaatctgatg ttgtatgtca ttttccatct tgcccttgag acagccatgc
     2101 ggcagggcga aatactggcc ttacgttggg agcacattga tttgcgccac ggtgtggctc
     2161 atttacctga aaccaaaaac ggtcactcac gggatgttcc tctgtccaga cgtgcccgta
     2221 actttcttca gatgatgccc gttaatctcc acggcaatgt ttttgattac accgcatccg
     2281 gctttaaaaa tgcctggaga atagccacac aacgacttcg catcgaggac ctgcattttc
     2341 acgatctacg gcatgaagca ataagccgct tcttcgaact gggtagcctg aatgtaatgg
     2401 agattgctgc aatatcagga catcgttcca tgaatatgct gaaacggtat actcatcttc
     2461 gtgcatggca actggtcagt aagcttgatg cccgccggcg gcagacacaa aaagtggcag
     2521 catggtttgt gccgtatcct gcccatatca cgaccatcga tgaagaaaat gggcagaaag
     2581 cgcatcgtat tgagatcggt gattttgata accttcacgt cactgccaca acgaaagagg
     2641 aagcagttca ccgcgccagt gaggttttgt tgcgtacact ggccattgca gcacagaaag
     2701 gcgaacgtgt cccatctccc ggagcgttac ctgttaacga ccctgactac attatgattt
     2761 gccctctgaa cccgggcagc accccgctgt aaacatcatt caaaacacat acagaaaggc
     2821 gagttgcaac aactcgccaa acacattcga tctgcatgat cgaaattatt cgtttcacca
     2881 gaaaaaacac cgaagataca ataaccacat gagctgaaaa gactttttca gtcggaggaa
     2941 caatgagact gaatacgacc ggtattgcgg cccgaatgat gctctcgctg gacaaaaaag
     3001 caatcggcga aaaactcatc aacggtcgtc agataaaccc gacacctctg caggtccgtt
     3061 acccccaagg ggtaagagaa gttctgggga taatgagcga acaattagcc atatccaccg
     3121 ctgatttaac ccgaatcctg cttgaggatg ctcttcataa tatgtttatg cctgcggata
     3181 acaccacagg aaacataata agcagaattg aatacatcat gctgtcgcat gatataaatg
     3241 cgccattact ggcaacattg ctgtcgccat ggaatatccg ctccacggtt atacaggacc
     3301 cggccagact tgcagactat ctttcagctg acgctctcga acatcttgct gaatgtttta
     3361 atctcaatcc ggactggctg aatggccatg aaaactatcc gatagcgttg tccggtgaat
     3421 ggccagatac tgctgataat ttcagaatgc ttattagtga tagcagcaac actgaagtta
     3481 ttttctggca tagtttcccc ttcgcaggaa acacgaaaag agaatattgc ggggtaatat
     3541 tgagacagaa aaaagaaata aatggctctg taatatatcc ggcactatca ttatcaccaa
     3601 cacttctgaa cgatgaaaaa agaaaatggt taactgaata tacaaccagg cagaatacaa
     3661 caatgtcttt gcgacgcgtc acgttgagac ctggccttgc cgggaacctt attacagggc
     3721 aaatactacc tgttagctta tttaatacgt ctttattacc ctggtaaata ccagatagta
     3781 ccaaagtaaa aattacggca catccgtgtc atagcgttgt tttgtctaaa ttctgataaa
     3841 caggaaatta aatgatgaag aaaaatcata ttacccgcac catcattgca tctgctgtat
     3901 tattttcctt taacgctgca gcagcgacgt catattttga agcgcgtaac gatgcaatgg
     3961 gtggtaccgg ggtagcatct tcccattatg gtgtggcacc tctggcaaac ccggctttac
     4021 tgacaaagca caacagcaat gatgatttca gccttcttct gccgtctgtt ggggcacaag
     4081 ttgctgatcc tgatgatgta tcaaataaag ctgatgatgt taaagatgac tgggatttgt
     4141 ttgacagtgc tgtagataac cagcatggcg tacaacaggc agcggcaaat cttaaacatc
     4201 gtctgcagga attccgcaat atcaatgcag atgctcaggt aggtgtatca gctgtagcag
     4261 ccatggcaaa tgacactctg ccgtttgccc ttatggtcaa gtcttacgga actgtcagcg
     4321 taaacggaaa agtaaacgat gcagacctgg attatctgga caaagttgcc aatggaacaa
     4381 ttaccgacgt cgataagaat gccctgacct cacgcgcatt cgggcgtgca gctgtaatta
     4441 ccgacgtcgg aatttcattt gccaaagaac tggaaacggc cggacaaaaa tggtctcttg
     4501 gcgtcacacc aaaatatcag cgtgtggacc tgtttaacta caacgtaacg gttcgtgatt
     4561 acgacaaaga cgattttgac ggtgacaagt accacaacac gaaaaacgga ttcaacgccg
     4621 atattggtgc ttatacagac ctgaatgaca actggactgt cggtcttgtt gcccagaaca
     4681 tcattccacg cagtattgat actaaagtcg tcaatggctt taaagaaacc ttcaaagttc
     4741 gtccgcaggc aaccgccggt gtatcctggc ataatgacct gtttaccact gctcttgaca
     4801 ttgatctgac tcctgccagt ggcttcactt ctgacagcaa acgtcagttt gcaagtgttg
     4861 gtgctgaatt taacgcatgg aaatgggcgc aattgcgtgc cggttatcgt cagaacatgg
     4921 catcaaactc cggaagcgcc ttcaccgctg gcgttggtat ctctccattt gacgtcgtac
     4981 atatcgatgt ttccggcctg gtaggaactg accacgatta cggtgcaatg gcacaacttc
     5041 agttcacttt ctgattactg tccccgcccc cggcactaaa cccgggggta tttttatatt
     5101 cagtttaaat aacatgatga aaccacgttc ttcttattca aaaacagcat tcattctttt
     5161 attttctgtt ttcctggtag cggcagtgac aaaagcaaaa agctcgcttc ccgatatcac
     5221 cctggaacag gcaaaagaaa ttaatgctga taacaccgtt attttccttt tccgccatgg
     5281 agaacgttgc gaccgttcag atatgccatg ctattcggat aaaagcggaa tcactattac
     5341 cggtacagag aaagcacagc aggaaggtat taagttcgca acaatcttca gtgaatatga
     5401 tatttactcc agcaatgcgg taagaaccat acaaactgca aaattcttct ccggtaaaga
     5461 acccgtcgtt atggacagtt tatcagactg caataatgat ctttataaga cgcttgaatc
     5521 aatcgcaaga gaatctcata aaaggaacat agtaataatg acacataatc attgtttatc
     5581 atttctggcc agagataggc tcggcaagaa attcaaaccg gcgtaccttg atgccctgat
     5641 catgcattac gatggtaccc ggctcatact tgacggaaaa tacaacaaag aggcatagag
     5701 gatatttgaa tttacataaa ttctgatata ctcagtaaga tacaagagtg tacgggcaca
     5761 tgtgcctgtc tgaattgcag gttatccgga cgagggccgg gaactgtttc agggacaagc
     5821 gccggtacgt tcgattcata aatcgtcgag tcatccatca tggatcgatt cggcgcggag
     5881 cgttaaagat tacgctcata ttcgttttat gtctgacgac acatcaacca gccagtatca
     5941 ggaataatca ccttcagtaa actatgaggc tatttacctg gccacactgg catttatttc
     6001 cggtaacgca agtatagccg gaaacaaaca tcatcctgtc tgtaccggag caccggtata
     6061 tcccattctg atttaactgc tcaggaatta ttatgaaccg tattatacct gcggggtgtc
     6121 ttgccgccct ttttctggct ggctgcaact ccccttctca gcatactcct gttccgcgta
     6181 actacggtgc tgcctctgat gtggcggtta cccggcacac tcagtacaca ttatgggaca
     6241 aaggtgtatt ttccggccaa cttccggttt caacggggtc tcatcttctg acagctaaca
     6301 gcgatcgcct ttcctttgac tgggaaggtg atgcaattga attactgaac gaactggcca
     6361 gagtcagggg gatgcagttt aactacaatg gtgtccgcct gccgttacca gtcaaccttc
     6421 acgtccggga tatgacgttt tccaatacgt tgagacttat tgaagcccag acagcctgga
     6481 gagcaacaat ccatcaatat ccgggcttgt tacaggtcag cttcatgcag ccggagaacc
     6541 ggaaaaaatg aaagcactca cctctctcct tttgccagta atactgctta ctggttgttc
     6601 ttcttcccct accatctcgc caggtggtgt caatacgcca ccacctgaca ttaatgcatg
     6661 gcttaaacca cgaagtgaaa agccggatag tctgtcagaa acacgctgga aaatgctgac
     6721 agacgcaggt aaaacgcttg gatttcgtgg tggcaaggct cagcgttcat gggagctgat
     6781 tcaggcgctc aacgcccggg aaagcacact taatgcctta tacgatttcc ggccactgat
     6841 tagcccggaa ggctggctac caccggttat tgatgaagca caggacgtag ctcatatcac
     6901 gccagatcaa atccggacct cttcccgggt ctggaccatc attcgccctg aacgctttgt
     6961 cagcaatcca ccgggctggc gtgactggct tctccggggg ctttcaacaa cagccactcc
     7021 gggtacagaa ggcagcgtcg ttccagaaga cagcgtgcaa cgaaaagtgt gggaaaccgc
     7081 attacggcag ggatggcagg aaggaagaca aaacgccgac ctgacgcttg aagccaacca
     7141 gaaaaccctc acccgtgatt accggggcat gatgctgtac tcacttctct ggcgtcaggg
     7201 catgatcacc cgtccggatg tttctgatca aatgcaaacc gttacaggtg acgggaaaaa
     7261 actggtcact ggagatcgtg ttcgtcgcct taaaaatcat gcggaattca atcttcagaa
     7321 atctcactgg cgtccgctta taggcactga aggaggctcc cgatgaatat tcctgatgag
     7381 tttggtcttt ttccgtttca gcgtttcact gcagatgaac tacggcattt ctttgtctgg
     7441 tgtgccgcac ataaagtcag tgatgtggat ctgaccggcg ggtcacctgt atctgtcagt
     7501 cgctttggcc gacgggtgcg ttgttccagt gcaacgttac caaccacact catgtcctcc
     7561 ctgattgatg aactgttcgg tcgggaagtc attccccgcg ttctggcagg caatccggtt
     7621 gacaggacga tacagattaa cggggatgca tccgggcgct atggtcttaa acgcggagaa
     7681 cgcatccggt tacgctctca cctcattcag ggcacatctg gtgcggagga gaaagcaatc
     7741 tcaataacca tgcgtgtcat cccgacagaa ataccggaca ttctctccat gaacattgaa
     7801 ccagacctgc ttgaagcaat ggtctgcaaa agcggtctgg gatttgtatg tggtgaaacc
     7861 gggtcaggaa aatcaacgct gtgttcagca ctgtaccgtt acattatgga caatttcccc
     7921 gacgccaaaa tcgtgacata tgaagacccg gtggaatata tcctgggaaa tgaaaacgat
     7981 ctgctcccac cgcatcaggc agaaatcggg cgtgatgtgg tcagttttgc tgccggactg
     8041 cgttctgctg tacgacgtaa tcccgaaatt atcggtgttg gtgagatcag ggataacgaa
     8101 accgccgacg cagcggtaca ggcaggcaac accgggcatt actgcctgag caccatgcat
     8161 acgaaatctc ctggtgaaac gctggcacgt cttcttgggc tgtttccccc tgtcatcagg
     8221 gattcgatgg cctgggctgt cctgtcactg ttgcagttca ttctggttca ggttctggtc
     8281 agaacaaatg acggtggtcg taaagccgtc cgggaatata tcgtcattaa cgatgagctt
     8341 cgcgacaatc tttccggaat gccgcatgca gagtgggggc atcacattga tgccataatc
     8401 cggcaggaga agtgccgtat ccgggaccag attctggaaa tgtatatccg gaatgaagtt
     8461 gaccgaagag aagcgattct ttttatcccg ccaggggagt tacggtcatg aaaagagaag
     8521 gtttctggcg atatgcaaca ccgccagtca acatccaggg cgtacctctg ccgttcacca
     8581 tgatttatct gtttatatgc ccctttccct ccaaaacaac cttctggatt tgcaccggca
     8641 tcattttatt tttcgttatt ctcgaccgtt caggctggac tgtcaggact atctgtacac
     8701 gcattttcag cattctgaga ggggccatcg cctccggtcg tccatggtgg tatcgccacc
     8761 atacggaatc accacgagac tggactggcc tcgattaaat tctggagtct ttatgtcagg
     8821 taaaaaaact atcgttactc tgctccgggt atcgctactg gcatgcccgt tactgttcac
     8881 gacaccatcg tttgcaatga tcgacacgcc atccgtgaag gtcggctttt ctcctgaagg
     8941 aagcgcatcc gctctggtgc ttgacacaat caacagtgcg gaaagctcca tcaggatgat
     9001 ggcatattcc ttcaccgacc cggatgtaat gcatgcgctg gcaaaagcga aaaaacgcgg
     9061 agtggacgtc cgtattgttg ttgatgacaa ggggaatacg aaccgggcaa gtcaggaagc
     9121 gatgaaatat atcaacctgc tggacattcc tctccggact gtagatgcct tccccatcca
     9181 tcacgacaaa gtcatcattg ttgacggaaa cacggttgaa actggctcct ataacttctc
     9241 tcgtgctgct gcacgcaaga attcagagaa tgtcgttgtg ctcaagaata tgccggatgt
     9301 agccgcacaa tatcttgaac actggcagga tcgctggaat aagggtacag actggaaacc
     9361 ctgacaaaca tttctccccc gattttctat ccccccagcc cccccggctt ccggcggtgg
     9421 tttttttccg tcttttttca ggagaagcac tcatgccgtc tgtatcccgc taccgcacct
     9481 ggcttgccgt gcctgcagat gaaatagaag atctcaaaaa agcacatccc ccgatgaacg
     9541 ggcacacgcc cgtcatctgg gacaaggagc acaaactctg gttcgcccgc ccgggggcgg
     9601 acctgtccag gctggaccgc tggctgcccc ggccacagga cgtctccatg aacggcagtg
     9661 atccggtcac cgagtttgcc caggttctgg agaatgccgg actggttctg aaagaactgc
     9721 cggtcatgga cgggaaaatc caccgtgtcc cgaccgccga tgacaaaaaa ggacaaaaaa
     9781 gtggcgcata tcgcggattc ctggacggca gacctgccgg ctggtacagg gattatcgca
     9841 gcgcggatga ttcgccgatc acctggacct tctccggcgg agaacagaca gatccccgcg
     9901 caagactcca cctgaaagcg cattcaatgc aacgcaggga ggacgcggag cgggaactga
     9961 aagcacagta caaccgacag gccgcgtatg cccgccgcta tgtcaacaaa tggccgcagg
    10021 ccacagcaca tgaatacctg acccggaaag gcattcaggc tgcaccgggg gtgcgggtaa
    10081 acaacaaaaa tgaactggtt attcccttca gtaaccgtaa cggcgcgata cgctcttacc
    10141 agcgtattcc ggtcaccggg gggaaggatg cccgcatcct gaaagattca gagaaaaccg
    10201 gtaactggtt tgcgctgggc acaccgcgca acggtcagcc ggtactgttt gccgagggct
    10261 atgccactgc ggcctccctg cacgaagcca cgggccttcc ggtactgatg acggtggatg
    10321 gaggcaacat gattgccgtt gccgaaaatg cccggcaaaa gtggacgcaa agccccttta
    10381 tcttctgtgc cgacaacgat catgcaatcc gggttaacaa aggtatcgtc agtgcaacaa
    10441 aagccgcaga actgacagga ggaacggtca tctttcctgc gttcaccgat gccgaaaaag
    10501 cgcaggggct gaccgatttc aatgacctgg acgcaagccg gggacgggcc gcttttcagc
    10561 atgtcatcaa cgcccagtta gaacacatcg gggtttccac ccccaacagt aacaccccgg
    10621 aaatccgcga ggcactggtg atcggcaacc tcgtttttac accggtccac actgaggaaa
    10681 aaaccatgac tccgacagaa tatcctgaaa catcacccga tacagggcac agccatgacc
    10741 agggaccttc tctgccgtcc gccacgcagc aggaacaacc agctgcttcc agcagtaaca
    10801 ttgctgatga aacacagtca tttaccagtc acgccactga aaataacggc aaagatgagc
    10861 ggcacgctga taacgttcag gcgcaaacgg aaagctctgt agttcaggac tcccctgcag
    10921 aatcaccggc gtccccggaa agtacaacgg catctgcccc tgatgaaccc gctcatccgg
    10981 cggaacctcc ggaaaaagtt gtctcggtgg ccacagataa agactggcgt gaatttgaag
    11041 ccgaactgtc acaaccggaa aaaaacgagt cacaacagga atccggcact tcacctgaaa
    11101 taccggcccc tgctgccgcc cccgcatccc ctgaagacag tccctcatct gaaaccatga
    11161 atgaatcagc cgagccatca ccttcccctg atgcagtcaa agaggaaatg accgtgatga
    11221 cggagcctga tgacaaacaa agcaccggaa tatccccgga tactccccgg gaagaggcgg
    11281 tcaaccatgt aactgaagac attccccttc agccagagcc gttccttcct gagtcagatg
    11341 gccccgaaga atacgaaaac tacagcgcat atcaggagct gatgaacaat gaaaatcctg
    11401 agcatctgca ggaaaacagt gatatgccgt cgcaaccgga cacagaccac gttcaggcag
    11461 atgagtcaga acttcaggcc acaacagata ctgcagaacc ggctgtccgg gatgaaccca
    11521 ctccacagca acctgttcag agcacatcac cttctgataa tacttcatcg tttctcgaca
    11581 aagcgcgtgg cttttttacg cgtaaaaaaa cggattcaca ggcacatgag aacagtgatc
    11641 ccacgccctc agcggaaacc acgacgactc caccgatacc tgacagtatt gtctacgccc
    11701 cggaacgttc tgatgcaccg atatcgctca acatggacga aattcttaaa gagcttgact
    11761 gggaaaaacg agcagacaga accgtcctgt acaaactgga tggcaaacct gccttcattg
    11821 accgggtaaa ccggctggaa atggtcaacg gtgccagcaa tgacgatcgc agcgttctgg
    11881 ccgcacttgc tgtggccacc aaattttatg ggggcgtcat agagctgacg ggcagtgatg
    11941 cctttaagca aaaagcaatg cagttgatta tcgagcacaa catcgatgtc cggatgaaat
    12001 tgccagatca acgggctgaa ctagaaaaat tacgcaagga aatggctgtc agtaaggatg
    12061 cagttgtcac ccatcagcca acaccggaac ttaaccggaa taccccagaa caaccggctg
    12121 tccctgaccc cgttcaggaa aaagaagcca cacaatcccc ggcgtcccct gttgcccctg
    12181 aagcatcaac cgtcagcacc gcctctgcac catcaccggc tgaaccaggc aaaacggcag
    12241 atgtggcacc agacgaagaa ccaccaggaa aacttcgccc gggagagagc gtgactgccg
    12301 tactgcacaa ttttggtcgt gctgaatatg cacccggcaa aggagagagt ttctttgttg
    12361 agcttaaaaa ccgcagtggc agcaaactct actgggggga acaactggaa agtctggtca
    12421 aaaaccatca gaaaggtgac gtggtgacac tgacacttca gaaccgcgaa caattcatcc
    12481 tcccgggaga gcaaaaagca cgtttccgga ataagtggtc catggagtca gttaccaacg
    12541 ggatctcggt atcacatgat aatccggata aaggtcagcg tattcaggcg attccggtcg
    12601 aaacctttat gaaagtggct gcgcaaatca gccaggggtg gcccgaagag atgaaagcgt
    12661 tacgtatgcc agaaaacgtc ggctcacatc tgtttatcgg tgaagaccga catccggtct
    12721 ccgcgccaca gaacgcgaat caggtaacag aaataaccag cgcagcccct gacaagctga
    12781 cacctgttct gggatcagtg gataaggata cgcgtgaact caacctgtta cttgtccaga
    12841 gtgccgatga acatctgcag ggtgtggtcc ggctgaacgg cacattgtat ccggcactgg
    12901 ccaccccatc ggccgacaac agtcagttgg ttatcaatgc gctgaccgac aaaggtttac
    12961 gctttgcagg ttatggtgaa gcggtaaacc acgatgctga cagcacgaat cgccccgcac
    13021 cggagcttat gcagtttcat ctgaaaaccc gggaagaacc gctttttgct gctgtctaca
    13081 cgccggaaaa acaacctgat gcgttgtacc gaaacctggg cttcgagcag tcatggcagc
    13141 agtggtccaa cagccaaaag ccggaggaca ggcaggaaaa aactctgcat caggatctct
    13201 cccacagccc cggccgatga gccggtaagg aagccatatg aacatgcata agtctctggt
    13261 tgccgtgctc ggccttatcc tgatttcatc cacggcaagt gctgcaacct gtcgtgcggg
    13321 tagcgcggca caggccggta gtaatacagg atatgagcga gcacgtgcag ctgcggatgc
    13381 atggtcacaa cgtgaaaacg atgtttcctc atccctgcag tcctgtctgt cacgaatcaa
    13441 aaaaatcagc ataaacctcc cccagttccc cagccttgac gacattctgt cgcagctgga
    13501 aaaccaggtc tgcgacgcgg tcgtggacaa ggtgaatgaa aagctgccgg ggaatattga
    13561 tccctggaag gactacaacc tatgaccgaa aataccgagc catcctcgcc atcacatccg
    13621 gcacctgacc cggaaatact gaagagcaca atcagggcta tgaagcagtc agaacagcgg
    13681 gcaaataatg tccctgcgct gataaaagcg cttttatgta cgggcacatg tcttcttatc
    13741 agtattactg gtaacgccat tcagtactgg cactcaacga atgtggagcg cgaatatttc
    13801 gctacagaca atggtcgtct ggtcaggctg gcaccaacaa atcagccagc atggagtcag
    13861 aatgacgcaa tggcctttgg cagccaggca ctggcgaccg cattcaacct ggattttgtg
    13921 cactatcgtt cacagatttc ctctctgtct ccgcgttttt ctgacgaagg ttttgccgga
    13981 tatgtgaatg cattacaggc atctaacatt ctggagacga tcaagaaaga gaagatgaat
    14041 ctgaccgcca cgacaggcgc tggcgttctg gtacgacagg gacaaatgtc cgacggcgta
    14101 tggttctgga catttcagta tcctgtccgt atgcgtctgg tcggccagac aaccagcaag
    14161 ccagaacagt cttttgtttt cgaaatcacc atccagcgtg ttgaccccag actgaaacca
    14221 tccggtatgg aaatccggca gatgatctcc cgtaacgccg gaccgaactc ctgagataag
    14281 taatatgcaa tcacgttatt tactgagcac actgctcctg gtgtgcagcg ccgccacgtc
    14341 tgcagataac gcaggatggc agaacgcacg cacaccacaa acgacaaatt ctgccagcga
    14401 tcacgttcag aacgcctccc aaaacaccgg aggtgttccg gcaacactgg taaaaggtga
    14461 gctgcctgcc cccgggcagg ccagcccgct ggtacaggat gcggcaagac ttgattctga
    14521 gctctccgcc gatgagattc gctcactacg cagtctcatg gcggataacg agcgggccat
    14581 caatgcgccc atcaccagtg ttgtaccccg tatcagctct ctgacagtta atctgtcccc
    14641 cggtgccagc cttcctcttg ttcgcacagc catgaacaac ctgagcgtag tgacatttac
    14701 cgatatcaac ggttcaccat ggccacaatc cgatcctcct tataacgcgg caccaaaact
    14761 ctttgatgtg cagtacaacg agaacatggt gacaatcaca ccattgcggc catgggcatc
    14821 cggaaacatt tctgtctacc tgaaaggtct gtcggtaccg gtcatcctga acgtaaccag
    14881 tggcgaaaca gatacgccgt catccagtca ggaaatggac agtcgtctgg atctgcgaat
    14941 accacggcag ggaccaacca gcccggttgt ctccatcccg acggataaaa tcgccctgca
    15001 tgacgcaaca ttacaggcat ttctggatgg tattcctccc cgggaccctt cagttaagag
    15061 gctgaaattc acaggtaatg tcccggatac aacgatctgg caacacggcg atgaccttct
    15121 ggttcgttcc cgggcgatat tgcgcgatga atttgaacag accttgtcct ctgccgacgg
    15181 aactcatctc tggaagcttc ccgtcacccc actactgact ttctctgtga atggccagtc
    15241 cgttcacgtg acaccagaac tggagtaatc atgagtgctg aacaggatgc cggtaaatcc
    15301 ggaaagaaac tggctgccct gcttgggctg agcagcatca tcttatttgg cggcagctat
    15361 atcgcgtttt ccaaactatc agggagtaac agcgatatgc agtcagccgt gaacataaac
    15421 agtgctgcaa gtggcggtac gcgcagcgtt accgaaacgc ctcactatcg tgaactgtta
    15481 agagcggata atgaacgagg ggctgcggcg gcagcccgca ataatcagac gttcattgcc
    15541 agtcttccgc aggggcttga tattccggac actccggaaa agcaacaaca gcctgcagca
    15601 aaaccggaaa actatgctta tcgccaggct tccggcacac cacaggagga tcgagcagcc
    15661 agtgaaaagc gtatggaacg gctgcaaaag ctgattgtaa gaataaaaga tcagcatcct
    15721 gccggtagta caccaaccat agcaacgact atgtggaata aatctccggc agaaacaaca
    15781 gggcagaatg gtacgcagca attcgcatta cagaatgcca gcctgtccac gcctgtagca
    15841 gaaaaaggga tacaactgat acctgccctg acccgtatac cggcatatat tgatactgca
    15901 gtggattctg ataacccgtc atccaaggtt atcgcaacca ttcccgctgg cccctgggca
    15961 ggggcaacgc tgttcagtcc gggagtcaaa ctggtgggta acggcgtgga gatccatttc
    16021 gaccggatga gctggaacgg catggacctt aaggtcaatg catatgctca acgggaagac
    16081 aacctgatgt catccgtggc cagcaatgtg aatacacgct ggtttaaaca tatcattctg
    16141 ccgtcagtgt taggaggtgt gggcagtatt ggcacacttt ataaagatgc caacacgcag
    16201 gttattcagg gtaactatgg cacggttacc ggacgcgtgg gaatgcccag tggtgaagct
    16261 gttgcgggcg ttattgccgg tggtatggcg gaaagaggaa gtcagatatt aacccgccag
    16321 gcagaaagtg agccctataa acaggtagaa gtgtatcagc atgaagtcgt atcaattctg
    16381 tttgttgacc cggtaatgac aaacgacgcc aggtcatctt ccctttccag tggcatttcc
    16441 ccttcagtta atcgtacatc acaggcggaa caacgctcgc aggcacgaat gcagacagcc
    16501 atggagcaaa gaaaagctgt gatgcaacgt cggtatgatg aacaaccgga gacaccataa
    16561 tgaaacctga aacagaaata gatgaaaccg gcagcttcgg tgaacccgaa gagaaacccg
    16621 cccccttctg gaaacgttcc gtctggggga tatctgtagc aacatggggg ctatgtgcag
    16681 tcgtgttgct cgcagccata tggtatctgt tcctgagagc gccatcggaa acaggcatgc
    16741 ctccgtttaa tgatgcggat gctggcgtac agacctggca aaccacacaa gaatcatcac
    16801 cttccgttca gtccactgag actatgactg tccggacagg agaagatatg agccagcttg
    16861 cccgggatgt aaaaacagag ctggataacc gcgatgaaaa aattcaggca acactgaata
    16921 tgcttcacga cagcattaac aagctgggcg aagctataaa aaaggatgaa gaatacgcac
    16981 aggaaacacg ccgtcagctg gatgatatcc ggagccgcct gaacggaata atgacacaga
    17041 aaagcgtcac tgaatcctct tccacaccgc atccagcaaa gaaaaaaacg tcgtctgtac
    17101 ttaacggaat gaaaatcatg tcgatggaaa ctggtatggc ctggattcgc tggcagggta
    17161 gtacctgggc tgtcagagaa ggtcagacgc tgggcaatgt ggtgatccag cggattgatc
    17221 cgactacccg gaccatcatc accagtgcag ggacgttacg ctgatgaaca tggatgccct
    17281 gaccgccatt gagaactttg ccagcagtat tttcagtgcc ggaatggatt tcctgtttac
    17341 ctggggagaa ttcatcgggg tgatcagcat gattaccctg tttgcccgcg cccgttcagc
    17401 cggtccggta aaaatgtctc ccggaaaatt tattgcgggg atgttaacca gctgcatgct
    17461 cgtatcactc ccggcaatga ttaacgccgg aggcgtacaa atgggattca gggctgactc
    17521 atttggcccg attgcatatg tgcaacctca gacgtttggc gctgctgctg gcgcagcaaa
    17581 tgcagttctg tcgctggcca agctggccgg tgtgggattt gtcatgaacg ggatatccat
    17641 ctggcgcaag gccggactcg atggacatac cgcactgagt gccagtgaat ccgtcagtaa
    17701 gggaaacgtc aaatttattg ccggcgtcct gctggtcttt atcgatcgtg tcctcaatgc
    17761 cctgctggca agcattggaa tcgtcttctg atttttttca ccttcatata aaccactgat
    17821 atatccattt aaggagaaac tatgtcacgt attcgtgtcg tagcggcccg tctgtacgcc
    17881 gcatttatcc acgcacaact gtttgtaatg tcatgccgga gcaaactgac taagttcctg
    17941 ctgctcattc ccatgatgtt gctgcccaaa gccgttcttg ccgatggaga tcttgccgat
    18001 atggttcgca atgtcgaaca aggtgccaaa acagcacagt catcatcact gactattgcg
    18061 cagttcatcg gcgtaattgc gtttctcggg ggcctgattg ggctgaaaaa agttgggaaa
    18121 caagggggga tgggactggc tggttgcatt ctatctatcg tcattggggc gctacttgcc
    18181 gccggacctg aaatgatgag tcgcagccag aaacagctgg ggatttccac aatttccatt
    18241 ggctaatctt gtccagggca ctggcattta tccggtgccc ttcctgttcg gcaatacaag
    18301 gaggcacata tgtctgcctg gagcccggag cacataacgt tacgacgaac agaaaacggc
    18361 aatgtgattg cccacgacag tcgggacgat caggaattca tttttgcaga atgctgttcc
    18421 gatgaaatcg caaacatcat tctggaagcc gtgaaacgtt acaccagtac ggagcctgct
    18481 catgcaacac agcattaaag acttgtggtt gtatcccttt ccagaaattg atgtggttca
    18541 tacacaggag ccactgttac cggaaccgga attaacaacg cccggtcgat gtatctgctg
    18601 ccggcaaaac gtcaggcacc gtttcagact ggatgactca tggccactca gacagcttac
    18661 cgacacaatc agtgacacac gagttcgtct taataaggcc acagaacatc tggataagtt
    18721 aaaaaagcgt ggtgaaccag tcgcaaccgg cgaaaaggaa aagtacaaca cggcagtaaa
    18781 agccgctgaa cgggcgcttg agcaggcccg tctgtctgcc agacgtcttt cactccgcca
    18841 tgtacagaaa gctgaaatca ccagtacaga gtcgttgtca gagaaagagc aggaactgtt
    18901 tcatgaagac ggcccgcctt acagcttatg tgctttctgc cacgcctggc attcactgaa
    18961 tggttatgcc gcagcacagg gcgttatggt gtggctaccc gaccttcatc catccacggt
    19021 cgtggccctt aaccgtcgtt cattacagga agttttcagt aatgataagt tccgggtccg
    19081 gcggggacgg gaagcgctct ctgcactgat gcaaaacaga ctggcggttg aggataaatt
    19141 ccgcagtttc cgcccggccg atttcgctga cgtttttcgt cgctatccac cttccggtcg
    19201 ttcgcccctg cgtgaaaaaa tgaacgggat tgccctgatt ctgacaccag attcattcat
    19261 caaaaaagaa tacgttgatt gatttttctt catgccactc cagcgcgggg tggcattaaa
    19321 aaaatcaaac tgcacaaacc tccctcctgt aagtccggag ttccctcacc tatgaacata
    19381 aaccgtttaa tcttcactat cgaagactgt ctgaacacgc tatcccgttt ctctgtcgca
    19441 agcagttttg ttgagtattg cgatttacgt actgtaatag gtctggacag acaggatcgt
    19501 gaacgtcgcc catggctgaa ttcgccctat attgcagcca caaagcgtgg agaatatctc
    19561 tctgtctttg aagtcagcgg tgctttcagg gaaatggatg aagcatccga ccagactggc
    19621 ccaggttcac tggaatcgct gatcaccagc atgagtgaca gcctgaatac ggcatataaa
    19681 aacagcggtc ataaaatcag cttcgttttt gagcgtgatc ctgacatggg gaaagaagaa
    19741 atcgaagaca tggtcgcccc acaaaagcgc tcactggcta atacaggtat ccagttacag
    19801 gatgtggtcg atgagaaagt cacaacactg tccccatggc tggtccggga gcgttgctgg
    19861 ctggcgatct ggagtggacc cgaccttatc agcaacagcg atcgcaccgc ccatgatgag
    19921 cttgtcagaa gactggctga acgggtaccg aaagcacgct ttgcccagtc cccctggcag
    19981 tggaccttat ctgcactgaa aatccgccac gaagcttttc ttgataatgt ggaacaggca
    20041 ctgagacatt catctgatgg cctgattctc cggctactgg atatacacga ggtcgggcgt
    20101 gaaatacgtc gtcagacaga acggcacagc acaccgcgta actggcaacc ccacctgcct
    20161 gaagatgccc agccagcggg atatcgctgg actgacgatg aatccgtact acatgctcct
    20221 tcattgcatt tgcagttatt caacactcag gtgaccacac agggcaacct ggttcaggcg
    20281 ggcggactct ggcacggaat ggtatcgatt actttacctc cgcaaaatct gcagacgttt
    20341 aacgaactgg tccgtgccgt cccccgcgca gtcccctggc gcatcagaat ggatctgatg
    20401 cccggcggga tgaaggctct taatctgaag aaaacgcttc tgacttacag cagtttcatt
    20461 tctgccgttc gtccaatgta tgaatccgtt atggccctgg cggcaacgga tgaaaaagaa
    20521 cctgtctgca taatgaccat catggcctcc acgtggggaa aaacacgtga aatatgcaca
    20581 cgaaaccagg ccattctgaa atcagccatt gaaggctggg gcgtatgtgg caccacgaca
    20641 acatttggcg atccccgacg cgcctgggtg aataccattc tcgcggcatc cggaggctcc
    20701 ggcccggttc ccctgtatcc cccgttgtca catgccatct cgcttttccc gcttaacagg
    20761 gcaggctctg tctggcgagg caaagggaat ctgatgttac atacagaaga cggttccgca
    20821 attgaggttg gtctggcgtc atcccaacaa aataaacata cagaactcgc accaggcgat
    20881 ccgggactgg gtaaatcagt tctgattaat acattgtcgg aaatacagat atcatctgct
    20941 cagaaaaacc tgcccttcat tgcctatatc gataagggat acagtgctca gggactggtt
    21001 cagcttattc gcgacagtct tcctccggaa agaaaagacg aagctgtcgg tattattctg
    21061 agcaatgatc cggaatacac ccgtaacctt tttgacgtga tgtacggcgc taaaaagcct
    21121 attacgccgg aaaagaattt catgtcatcg gtgctgtgtg ctctctgtgt cgataccggt
    21181 accggtcaac cctgtaaccc cggagacacc cgacaaatta tcaaccagct tatcgagctg
    21241 gcttttaaag agtacggtga aaataatccg cgactttatc gcgcctcaac ggaagaactg
    21301 gtggattcag cactgcagga ctcgggactg tatgaaaaac atgatgcagc ctggtgggcc
    21361 agatcaactt ggtttgaagt ccgggatatg ctccacaatg ccgggtatat catggcggct
    21421 cagcgggcac attatcaggc aatgccccag cttcccgaag tgtcctcgat gctgggccat
    21481 accagtctca gggatgtttt cggtactgtt cagcgtgatg gttcaaacga gcttctgctg
    21541 gactacatcc gccgcgccct ggagcaggga cataacgact accccatgat atccgggtat
    21601 acccggttta tgatcaaccc ggaaacccgg gtcatcgctg tcgatctgaa taatgttgcc
    21661 ggtgacaaaa cgcctgcagg caggcttaaa accggcatca tgtacctgct tgcaggtcag
    21721 atcgccggtg gtgacttcac cctgccacaa tacagggatg aagtgttaaa gcagcttcca
    21781 cgggaatacc atgaaatcgc tcttaaacgt attaaccaac ttgatcagga ggttaagaca
    21841 aaagtttatg atgaactgca taacgcccgg ggcattgact tcatctggga aaatctggat
    21901 acccaggagc gtgagcaacg caagtttgct atccgcacgg ttctgagtac gcagtatctg
    21961 agggactacc cggaaagcgt tcttaagtca gcaaatactc tctggttact gcgctacaaa
    22021 cccgaagata tccccgtact gagggataac ttcaacgttc ctgaatttat gctgaaacgc
    22081 ttcctgaaaa tgccggaagg cccggcacca gatggtagtg gtgttcctgt tctgggagta
    22141 ttcagggtaa aaagtggcac gcttgcgaga atactcaaat ttaccgtcgg tccactggag
    22201 ctatgggcgc tgaactcatc gccaaaagac agtgccctgc gtaaaacact gacaaataag
    22261 ttaggttcag ttcgagcacg taagattctg gccgaaaact tccccagagg ttctgcaacg
    22321 tcattaattg agcaccgggc gggccaacat aacagtgaca atgttattga agaactggcc
    22381 tcggagctca tccgaaaaca gggatacaat ctctgatgtg tacaattcat atcaccccac
    22441 gctgccggag acctgccaga gggcaggtat ttcttaaaaa acttgaggta aaaggtgaca
    22501 gtttctcatt cactctgccg tcacgtgtct tcaggttaca tccggctccc aatcccagag
    22561 tgatatacgc ctcggatgcc agaacacagg gaaaggttac aggcatgcgg ttgttgttgc
    22621 cgtccagcac agatgagctg ataacaacgg caatatggca ggtttctgtc ccggatgaag
    22681 caccattcga cgttcagcat gtcatccagt ggatactccc ttcaccgctg gcttcgctga
    22741 tgtccgatga tacctggtta tggccatccg ttcccggaac gccggcactg agtgccggtg
    22801 actggcctgt tatggatact gacccttcca ccttacggcg cgtcagacgt cgttctttca
    22861 gcatcatcga taaccggaac aggaaaggtt atatcaccag gcgatttcag tgtttcccac
    22921 tggccccact acctgaaaac agaaaatacg acagccttcc tgaccttgtg ctccatcccg
    22981 agcaggagac aataaaatgc aaagaaaaac attactcgca gcccttatag ccaccctgag
    23041 tggaacggca tgccaggctc atgcttattc agtcaccgtc gtcgccagcc ggcctgttga
    23101 agagcaggtc atccccagaa tggaagccat caaagatgtc cttggcaaca tactgagtac
    23161 ccagacagcc acgggtaccg ctatcaatca gaactctgaa aagctggcaa gtgtcattgc
    23221 ccagaacgga caggcgacac gtcagcagat gattttcagt aatgaaactc agcgcctcga
    23281 ggaggcacgg aaaagcttca ccgtgccaga ctccatctgc agtgaatccg catcagggat
    23341 tgcaacagaa agcaaaagtg cctcagcatc cgcagcctca aaactcagta aaggtggcgg
    23401 tgtgagcaac cgtagcatcc gcgaccgtct ggccagcgca gcaaactctc ccgtgcggga
    23461 agcttatgat ggcgcggcca tacacgccag ttactgtaca gaagcagaat atgcacgttt
    23521 tggaggaacc gccgtttgtc cttctgtggg cgaaatacca ggtggagaca gtcaggtacg
    23581 ctcgatttac cacggcgcag gtacagcgga cacacctgca gcactgacat gggatcagaa
    23641 gcaaatagat gcagcgacgg cttacatgaa aaacacttcc cgcccctctg ccggtcgtgc
    23701 tctgggtaaa ggagaagtga atacccagag tgggcgaacc tacgtcggac tccagaacga
    23761 atacaacggc atcattgatt ctgcgtcgaa tccccaactg acgctgattg ctgacagcac
    23821 gcctaatgaa agcacacgta aagcactggc tgaaacactg cagtcagact cagcggccgc
    23881 ttacttcgac caggtggctt caccagaagc aaaagcacgg ggatatatga gtacccgtga
    23941 gtttgaagct tttgaggctg gccggcgcta cgccaacaca gcatacctgg tggatttaca
    24001 ggaaatgcag ggagataacc tgctccgtga actggtaaga atcactgcgc aaatgaactg
    24061 gcaactgaac gacctgaaag aacagatccg ccagggtaat gtcatctctg gacagcaact
    24121 ggctctgacc gcccgccagt attatgaaaa acaactcggt agccttgaga aaacaatcaa
    24181 tcaggcaaac gcacgttgaa agctgacgtc agaacaggac tgaaaaaatg aataagttac
    24241 cggaaaattc agaccagacg aaaaacgtca aaaaatcacc atccataacc cgcatagcca
    24301 cccgggggat tattaacatt tttgttcctc tgatggagac tcgcctgata acgaattctg
    24361 tacagcatat gctggaacaa caaaaacgca ggctggacag atttcgtcag gtaaataata
    24421 aaaaggcgca actccacctc acgtgggaag aagctctgca ggccagccat atgtcagtag
    24481 acgatctgga tcgtcgtttc cgccggcgca gaacagtatg gcgattctgt tgctggagtc
    24541 tgctggccat cgctttgttt ctcagtggta tgctctttgc cgcctcttca ttacctctta
    24601 cgactctggt acgcgccatc agcactcttg tgttgatact gtcaggcgtc gctctttgtg
    24661 cttccagagc actgattgtt acataccgcc tctggcagtt gcatgaacga aaagtcagcg
    24721 aacccgagca ggggactttc cgtgattttc tgaacgaccg caatggctgg cgcaatgcca
    24781 cattaattgc cgtaaccagt aaacaatact gatcacgtcc agatgccagt accactggca
    24841 tgtcgccatc ccccttaatt aaatcctgtg tggcctttgc ccatacgcaa ttctattccg
    24901 gagttactgt gaaaatttta cttcgcgcac tctgtgcagg ccttgctata tcctcactgc
    24961 cggcaatggc ttcagtgacc tatcaggata ttgtgtctgc cgcaaccaat cctgacgatc
    25021 tgtctcgcca ggcgctggtc acgatattcg gtgatgtagt caccaatcca ctgtcaacca
    25081 gcgcccccac tctgatcgga agcatgttcg gggcgttcaa cagtatcatc gccgtacttg
    25141 ccgttgtctg gtttatgttc attggtatcc gtcatgttgt gcgttcgggg catcagggac
    25201 aggtattcag caccggacgc gatatcgtcg gcacactgag tgtcgttgca ggttttctga
    25261 tgatcgttcc aaccggaaat ggctggagta tcgctcagct cattatgtta tggggcgcat
    25321 cgataatggg ggttgggtcc gccaatgtga tggtgcaact tgccgctgat aatatcgcaa
    25381 atggttattc aatgactgta cagccagttc aggcatccac acgaacagca gcgcgtggca
    25441 tttttgaaat ggaactgtgt aagtatgcgg ttaacgcagg actaaatgat tttaatcaga
    25501 cggtcaaatc ctcgacttca ctgatgacag aatcagctaa aaccgccagt ggtaattaca
    25561 ctgtcacggt atccaatggc agcggtatct gtggttcagc cagcctgtca gtagaaggaa
    25621 acggaacaac agatcaatcg acgattggaa aattctttaa tcccttcagt aaaaacgaat
    25681 acagcggtgt tatctcagca caacgtgcag ccatggataa tatgattagc gacatggaca
    25741 acgctgccag tgagtttgtg acaacatttc tggaaaaaag aaattctggc aacggaatat
    25801 tgccggatat cgagacacgg attcagagag cagccgacga gtatgaacgc gctgtacaaa
    25861 aatcgctacc tattgataac ggagaacaat ccaggaaaga agctctcaag tcttatctca
    25921 caacctatgg atgggtgacg cttggcgcgt ggtatcagac ctttgccact gctaaccagc
    25981 gtctggcaga gctggctgac agagctccag ctgtaacttc aatgtcatca cttggggaag
    26041 ttggcgatac tgatttattt agtgctgtta tgggggcata caagactcaa ttacagaact
    26101 ccacatatac tccaacgata ggaacgataa caactcaaga tgaaagtaat gcagctaata
    26161 gcacagaccc acaaagtgtg gtcatgaaaa gtatagcgcc aaccatatta aaatggacaa
    26221 accaagctgc tactgaatgg tcaggaacag gaacaaccag caaccaagtt aacccattaa
    26281 ttaagatgaa aaacatcggc gattatacat tgggtacaac agaaattctc tggacaggat
    26341 atacaactgt cagagttctc gcgacaatgg gtgataactc tattttcgga aaggctgtta
    26401 atttgttctc tggattacct aaaggatttg ttgctctgct ggatgctgca gctcccccaa
    26461 tatatttctt gttgttcctg ttgttttgtg cgggtttttc cttatcaatt tacctaccat
    26521 ttataccatt cattttctgg atgacaggca ttgggaactg gatagtaagc gtattaatag
    26581 gatgcactgc cgggcctctg tgggcagcaa cacatctagg aacatcagag gacagaggaa
    26641 gtcgagcagc ttacggatat atatacttaa ttgacagcat gataagacca cctctcatgg
    26701 tattcggttt cttttttgcc tctgtcgcca tcattgccgt cggcacaata ttaaactcac
    26761 tgtttggcgc agcactagtt aacgtacagg taaaatcatt aacagggata ttcagccttg
    26821 ccgggttcct gctaatttac gcccgtatgt gtacaacgtc tgtagctgca atatttgctt
    26881 tacaagcata tcttcctgac catgttatca atttcttggg tggtagagat ggtgtaaata
    26941 ctcttggcaa cctcacctca tcagtaaaag atatttttgc aggttctaat agaaatatta
    27001 gacacagccc tggagttaga gaggacagac taaaaaatgt cacaaaggat gataataaag
    27061 atggaataaa aggataaatt taataaaaca accttgccat aaacactcaa tcgcattaaa
    27121 atatcgatga gtgtttatgg agggtttatt atgcaaatgg taaaaacaat caatagatat
    27181 cctgcttggt ggacattata ttacgccctt agatttatat atttcataag cataccagtg
    27241 ttatccattt ttattctttt cggagtgctc tccattacat cttcaagata tgtgacgcaa
    27301 gaagattata tttatacttg cgtctgtctg tttttactaa ttgcccctgc aatattgatg
    27361 tattcccgag caagctcaag aaaagacaaa ataaaaaaaa ttgtcgcgga gataaaaaac
    27421 accggttttt attcacctga caaagaatat gagggactgt cttttactca gggcgtctat
    27481 tttggcgtgg ataccaaaaa aggcactatg ctgtatgctc gtgcttaccc tggaaatata
    27541 atggatatta tcggctttga cattgacaac ttcacacgca ctgtgaccga cgccaagaca
    27601 ctagagattt atacaaaata cataaatata ccaatggtct caattccgtc aggatgcatc
    27661 catcccaaaa tgatggcgga tacaatgcat gcaatggcag aaagaggata tgattaccct
    27721 gtcgactttc ctcgcctaat tcaggagaag cgtaaggagt gggagcagat tgcaggcatg
    27781 cctgtagccg aagtatttta agataagctc cctccataac agccggttca aatgagccgg
    27841 cttttgttca ttacaacaga taacttatgc cgttaccgat aaattcgacc aagtgcttta
    27901 atcgcagcaa tttttatttc catatcatca ctttgactca atctatgtaa ttcacgagta
    27961 atgttctccg cctgacaccg gccttcacct aatgcataga tcgcagcaag tgtaacgtct
    28021 tttgatttac tcttcatcat ttcaaacaag aaagtattag ctatcgcagg aggcatgctc
    28081 ataatcaatc ctcttcatga aataaacaat ggcactcact tacaaacagg agtccactat
    28141 cgcaagcgac tcaacttagc aatatctctc aatttgaatg aaaaaaacag atggcatgtg
    28201 acagggaacc gtccgcatga tgtccgtgac catttaaccc cataagctaa ccttgtcaca
    28261 aacgggcgca gaacagcctg ccagacagac aacaaaacaa aagtccaaga taaaacatga
    28321 ataccggtat ctcaatgcga agaaataatc tccgtagaaa cccccatgag aaaaatatca
    28381 tatgggagaa aatcgcaatt atcacagcca gcgcattcat tattttcgct gtgctctggc
    28441 attaattatt ttcaacacaa aaaggaaaaa ataatgaatc tggtagacgc ctttgtcaaa
    28501 aaagtaatat ccgggccata taaagaatat ggaaaatggt ggatagatgt tgaatacatt
    28561 tcatggggcg ttcccgggaa aacaagactg atgtttgagt caaaggagca ggcactggaa
    28621 gttaaagaag ggtacaaatt tttaacgtga gatctctttt tgttaaaggg caggaagcgg
    28681 taactccctg cccttagtta cgtttccagg cttatcagtc gacaagttcc cggatcagtt
    28741 ccagaacagc aatcagcgcg tttgcaacgg caactactgc cctgagaacg atgattaaca
    28801 gattaatcat ccgcttctcc atcactggag gaaatcaccg ttgcttactc ttacattgcc
    28861 tgtcacaacg gcactttcag tgttagttga aaagagactt cctcctgcca gagcactgaa
    28921 cacagcacca cctgtttgtt cagccaggac ttaaaatctt gcatccatag tcccgatgga
    28981 tatcacggca gaagtgtcgc cagacagagc taacctgcgg attataagga tggttgctat
    29041 caaatgaaca gctcgctatt ataagaacgt tagttgaaaa gagatttcct ccggtcaggg
    29101 ccacccactc tgtaacggaa cagtaaaaaa accgacctca ccagtcggtt tttttacatc
    29161 tgttgtttct caaaatccgc ccgacatgcc cgtcacaccg aggaaactca tgctctgaat
    29221 cagaaccaat catctcaact ttgccgtctc ctgaaaaaac tggcacattc agttcgataa
    29281 ctacccataa atacagagag aaaatccgat gtcagaaaag tggagtaaag aattcgactc
    29341 ttgctttcag gtggggagca tagaagaaat tgccgatgct ctcgagaaac gcagcggaag
    29401 cgaggaaaac gcgttagcca tgctggcaat gacagccttc acactcatga cccgacgtgg
    29461 tatctgcgaa atgcagaacg tggcccccga tggcaaaggc attcggctgg agctaatcgg
    29521 cttcagggaa aatgaaacaa taccggatac gcttcattga agaagaaagg gcgcaatgag
    29581 gtcagccttc gcaacaaagc ggcggcagaa aacctgccgc cgtgttgccc gttaacgttt
    29641 aacttcgtag gctaacgttg ccacaagcac tgtgtttccc tgctggagcc ggagtccgca
    29701 aagcgaatcc ctcaccatcc agacaaaaca cagaatcgtc acacagatga cgattaacat
    29761 cattaaaaac gttcgctgtg gcatcatagc ccctccattg ccttacggcg gttatcgggc
    29821 tactataatg ttgtctaggc attacagtag agcccttggt tgattgataa aaaaatctcc
    29881 aggggctttc tgctgtatac cgtccacaaa acgctttcgg catacagcct caagcgcccg
    29941 cagctattct aacgactttc gtatctgcaa caatcctttt atcgcacggc taattcctct
    30001 ggttagctct tatcgcctgc aaataaattt ttaacctttc cgtttgattt aaattccaga
    30061 aatggagact ttgtcatgtc ttacaatcga cagcctgtcg cagaagatcc tatgcagata
    30121 tggggcgctg ttggtgtcct tcttatcctg ctgctattcg ttatatggtt attcttgccg
    30181 gaagtggttt acgccagctg cctgattctt cataccttat ggggactggt tgactgggga
    30241 ccatttcata actatgcagc accacgatac aacctgcttg ccatgaccgg taacaacgcc
    30301 gccaacataa gctatagcca gtgggttaat gtcatggagc agaccatagg tattctgtgg
    30361 atgtacctct tacctgtgac actctggtgt ttgtgggagt ggtatcaaca tccgggacaa
    30421 agtcgtttca cccgcagacc agtagatatt acccgactgc cacacatctt tgcttcgttg
    30481 tcccctgcta tcgcccctgt actggcagat ggtgatccgg aaaaactctt tcatggtggc
    30541 aaacgacctg aacggcgggt tgctctcacc ccggaagcat ttgtcgagca gcatactctc
    30601 atcaccaata tgcagctcga tgttgcagct gcaagacgat gtttcatggc ccagttgggt
    30661 aaacctttaa cgtcctggaa agacatggca ccacatgaaa aagcgctctt tgccatattt
    30721 ggtcttcagt atttcctgga tgatcgtaag gcagcattaa agctcatgga tacactgaac
    30781 ctctcatgca gaattaaaag caaacgggac agtgggaaat tctgtacccc tgtttattca
    30841 ctggccaaat cagctttcca gagagtgatt aaaagcaacg gagcacagca atggctgaaa
    30901 caacatcgct acgtccgcag tgggctggtc tggctgtatg cacatgacct gcggcttacg
    30961 ccacccaact ggatttggct gaaaggcgtg gacaggacgc tgttttatgc attacaccgc
    31021 gccaatacca cgaaaggatt tattgaaggc gctggcgtgg tggccgttgc acgtgcagag
    31081 gccgaagcca tgcgttttgg tcttccctgc cctgaaccct gtgttgatga agcggtggaa
    31141 ggcttacggc gggatatgct cagtctcggt cttatctggg atgaaccaca accagatcgc
    31201 gaccggaaac gccgcatcct gaccaactgg tccctcaccg acgacatcct gccccgcaca
    31261 cctgctacag acaacgaatt ctgattatcc ggagaactga aaatgacaat cgagtacttt
    31321 gcaacacgca ttgcaaaaca tatttccgtg caggcaatct ggccggatgg caggacagaa
    31381 attattgccg tccttccacg ggatgccctg tcagggctgg taacacgaaa taatcaactg
    31441 attcatatag cctttgacgc catgggcacc cgtaacagag aaaccgtcct gattgataaa
    31501 caggagcata acgctgacca gatacttaca gctattgatt cctgcctgcg ccttgaatat
    31561 ctgatggaac gccgcttcag cagaacacct ttgttccgtg gcataatcgc cagcgttgtg
    31621 ctgttcgtaa tggcaacgat tggcttctct ctgttccgct atgttgatcg tgtattctgg
    31681 gatgatacaa cgcccgaagc agtacagaca gcaggtgagc cacgactgct accccctcac
    31741 ctgaatcaca ctgtgccatt aaatgaaggc attcaactcc cggttccccc gaaaaaagac
    31801 gtccaggtcc cggagaaaac aatcacaagc aaaaatccgg aagccgcagc tgcgcgacat
    31861 aatctggcta ctgttctgaa gcggaacgcc gatcggggca tgtttaccat caacctgtca
    31921 tccggtcatg aacgaaccct gttcgcattt ctcgatcctg cctgtcctaa ctgcagactt
    31981 ctggagccgg ccttaaaacg cctggccagc gacttcaatg tcgttatcta cccggtttca
    32041 gttattggtg gagaggaatc caccgaccgt gtggcaccac tgctgtgtga aaaagatgct
    32101 cagaaacgtg ctgccggctg gcaccgactg tattccgcag ataacggaat gatgacgcca
    32161 tcagaagaaa caacgcctgc agatgaaaca tgcctgaaag cagccagagc tgcgattgat
    32221 gttaacaatg tcgccttccg taagttcgga tttgccggta ccccatgggt gctgtccgac
    32281 accggctggc atctgcctac aggaattctg caggaaactg gcacgcttaa tttattcctg
    32341 aaaaccaccg acagcgagtc tggccatgag tgaacatcgc gttaaccccg agcttttaca
    32401 ccgtacagcc tggggaaatc ctgtctggaa tgcactccag agtctcaata tctatggctt
    32461 ttgcctggtg gcaagtcttg tggccagttt tatctggcca cttgcattac cggcatgtct
    32521 gttgttcaca ctgataacaa tgctggtatt tagtctgcag cgatggcgct gccctcttcg
    32581 tatgcccatg acgctggaat gtgctgaccc gtcacaggac agaatgatta aaagaagtct
    32641 tttcagcttc tggccaaccc tgttccagta tgaggtcatt ctggaatcac cggccagcgg
    32701 catattctat gttggttatc aacgcgtcag ggatattggc agggagttat ggctgagtat
    32761 ggacgatctg acccgccata taatgttttt cgccaccacc ggtggcggta aaacggaaac
    32821 catctttgcc tgggcgatta acccactttg ctgggcaaga ggattcaccc ttgtcgatgg
    32881 caaggcacag aatgatacgg caagaacgat ctggtatctg gccagacgct ttggacgtga
    32941 agacgatgtg gaagtcatta acttcatgaa cggaggaaaa tcccgcagtg aaatcattct
    33001 gagtggtgaa aaaacgcgtc ctcagtcaaa tacctggaat cccttctgtt acagcactga
    33061 ggcattcaca gcggagacaa tgcagtcaat gctgccccag aacgtccagg ggggcgagtg
    33121 gcagtcccgg gccattgcca tgaataaggc actagtattt ggtactaaat tctggtgtgt
    33181 gcgcgaaggc aaaacaatgt cgctgcagat gctccgtgaa catatgactc tcgaagggat
    33241 ggccaaactt tactgtcgtg gcctcgatga tcagtggccg gaagaagcta tcgcaccgtt
    33301 acgtaactac cttcaggacg ttcccggttt cgacctgtcg ctggtcagga caccatcagc
    33361 ctggacggaa gaacccagaa aacaacatgc ttatctctcc gggcagtttt ctgaaacttt
    33421 cagcacattc acggaagcat tcggggatat ttttgctgaa gattccggtg atatcgatat
    33481 ccgtgacagc attcacagcg acaggattct gatggtaatg atccccgctc tggatacatc
    33541 agcgcataca acgtctgctc ttggacgaat gtttatcacc cagaaaagta tgattctcgc
    33601 ccgtgatttg ggctaccgcc tcgaaggaac tgattccgat gcactggaag tcaaaaaata
    33661 taaggggcgt ttcccttatc tgtgtttcct tgatgaagtg ggggcatact atacggaccg
    33721 tattgctgtg gaagccactc aggtgcgttc actggatttt gctctgattt tgatggcaca
    33781 ggaccaggaa cgtatagaag gacagacaac agccacgaat actgcaacac tgatgcagaa
    33841 cacggggaca aaatttgccg gcaggattgt cagcgagggt agtacggcca gaacgctgaa
    33901 aagcgccgcc ggtgaagaag ccagggcgag aatgaacaac ctgcagcgtc aggacggcat
    33961 atttggtgaa tcatggattg acagcccaca aatcagcatc ctgatggaaa gcaagataaa
    34021 tgttcaggag ttgattgaac ttcatcctgg cgagttcttt tctattttcc ggggagaaac
    34081 cgtaccatca gcatcattct tcatccctga cgatgagaaa agctgcagca gcgatcccgt
    34141 tgttataaac aggtatatca gcgtggatgc gccacgcctg gaccgtctcc gccgtcttgt
    34201 tccccgtact actcaacgtc gaataccgtc accggaaaat gtcagtgcca tcattggcgt
    34261 cctgactgca aaaccctcca gaaaaagacg aaaaattcgc acagagccgc acacgatcgt
    34321 ggatacattc cagcaacgca ttgcaggacg tcaggcggct atggcaatgc tggaagagta
    34381 cgacacagat atcaacgcac gtgaaagtgc cttgtgggaa acagcggtga atacgcttaa
    34441 aacgaccaca cgggaagagc gacgaattcg ctatatcacg ctgaacagac cagaactacc
    34501 tgaaacaaaa gaggaaaatc agatatcagt gagagcagaa agagcaggta taaacctgtt
    34561 aacgcttcca caggacaata accacccgac agggcgtcct gtaaacgggt ttcatcataa
    34621 aaaaaccaac cgtccggact gggacggaat gtactgaaca gtggccgcgc cggattctgg
    34681 cgcggccttt tttttaacct ggagaaggcg gttcccagcg tttatcctgc tccggcggag
    34741 gcagtgatgc gtcattgttg ctgttcgccg cgatataatc gcggtagtag cgttcacttc
    34801 cttcacggtg atgatccacg tccggtcgcg taatcctgta gtcttcgtga ccggtacggc
    34861 ctgtcacatt atgccaggca accatttcag caaagacctg gttaaattgt ttgtcgtttc
    34921 cttctggttc aaaacccatg cgtggatcac gcccgaataa taccggtgca atcaaatcca
    34981 gtttatctgc cagcgcattg cggtcatggt gattacggaa aacaaccctg tcaccataat
    35041 ccgtacagac aaactctccc gtccgacggt cacggaaacg aacactcccg ttcttctgca
    35101 gacgggcctc gagatcaccg gtattaccgt ataccggcgt tccgcccggt tcacaaagca
    35161 cgacacagcg gtctgtcgtc gttgtgcgtg acctgtcttt acggcgatcg cgataatccc
    35221 agcctctcag ctgcgataca gcagccctgt ctccctgagc agcctgaatt tcgacccact
    35281 gacggtaaga gggtggatac catttcccat cagcaataag tttctgtcgc tcatccctga
    35341 tgtcttcttt cagcctgatc aatgcctgca ttcgctgaac ttctgcaatg tgataatgca
    35401 gcttacgtaa tgccggatca tcgtactgcg cacgaatgtg tgacttccgc agacggcatg
    35461 cctgatgaat ttcccgacaa cgttccccat aacgcagatc tggtttacgc cattgttcac
    35521 gccatgccag atagcgggca cgcaggtcag cacgggcctc agctctggct tcacgtcgct
    35581 gccggcgcag cattgggtca cgtttactgc tgaccccgta cctgtcactg acagccagct
    35641 ccgggttata gcgactttca ggttgcaccc tgtcaaacaa atctgctggg gcactcacaa
    35701 aaggtccggc ctgtggctct gcacgcccca gtgtcagatc aggatgaatg ctgctggcct
    35761 tcaccggcgt ttgctcatga ttaaaggcat caacgacaac aagcccgtga tgttgtttct
    35821 gtagcatcag tccctgttgt gcaaacagct catgacaacg ctgccaggta aactcaccct
    35881 gtgtgatcat ttcttcaatc tgagggctac aatggctgat gctgtatccc cacagacttt
    35941 ctttgtcaga aaacagttcg cgtttgatag ccccctgcgg ccccgtgcct gtacgcatac
    36001 ggtgccattc tttatcggtc gcaggacagc tctccatacg acgaccacgg aagcgttctg
    36061 ttggcgtaac ctggcggaaa atatctgttg gtacaggctg ccagccacca tcgagttgat
    36121 tcacattgtc gagcgtcagc agcgaccata cgctgtcagc acgaacggga gtctggttat
    36181 gatcataacc gtcacagata accaggtgac catgctgaac acgcatccat aatcccgctt
    36241 cagccagtgt gcggtgaata gcctggcagt tttccagccg tccttcccgt gccatttccg
    36301 gcagacgttc tccaagatga cgacaggcat actgctgcag actttctgtt tcagcgattt
    36361 tttccgggcg aacatcagcg gcaatgaagt ccggattata gcgtcctggt gcttccacct
    36421 ggctgaaaat gtcttttggt actggcgtgt agtcacccat ttttttcatg agtttttctg
    36481 cacaccagga gggaccaaac gagtcaagct gtacaccttc cctgttgcga tcccagccat
    36541 ccatcaccag aaattttccg tccatctgag acaggtacag accatcttcc gcaagacgac
    36601 gatgcaggga taaccagtca tttacaggtt cactgcgtaa accagcgacc gctgtctgcg
    36661 cgacatactc gcgaaaagtt tgttttttcc cgcgtgtcca cgcgttctgg cgatcacgtt
    36721 caacggcggt tttgcgaacg atacgatttc ccggtgcatg gacccagcaa ccgttatccg
    36781 gagcaaaacc atgcttcagt tcaagctcac ggcaggcacg actgagtttt tcctgactcc
    36841 aggataaccg gttcagataa cccgtttcag ggtgtacccg gttaacagcc acatggacgt
    36901 gcagattatc tgtatcagta tgcacggcag agacatactg atggtcggca aggccgagtg
    36961 atttcagcgt atgacgtaca ctgtcataaa tctgttccgg acgcgggctt tcatgtgact
    37021 gccaggaaag gatgtagtga aaaacaggat cagtgtcatc ttttgcatag tgtgcctgcc
    37081 gggcaatgta ctccatatct gcagcggcag tttcaagaga agtacagtta tgaaagcagg
    37141 tgacaccata aaagttgacc cattcgcagc cgtccttcat gacatcaacc agcgccacga
    37201 atgactcatt tcgaagacgt gtcgcataat caacaaggcg actgaaacgg cttcggtgcg
    37261 gttgttcagc ctgcgaagaa gaagataaat ccagttcctc atcggtcatg tcatcccgga
    37321 cagagacgta ggataccagg tcttcgaacg aagacttgcc gtcccttctc tttttcggaa
    37381 tgactgcatt caccggagaa tcctcaacct tccattaact gtttacgcaa tgtatttgta
    37441 agttcggtta tggcaacaag cacttctgcg tattccttgt caccggttct ttttccctga
    37501 acaaacagat gcttctgcat ccttcccagt ctcattaact ccttcagaaa agcatcatct
    37561 gtacgggagt taattcgctt attcagtgct gcatttcgga tgtaagcaga tacagtaagt
    37621 ccggaatctt cagctttttt cctgatggtt tcatcctcaa caggagagaa tctgagtgtc
    37681 ctgacaactg tcttttgccg tacctcactt ttctttctta ccgcactatc actcacttca
    37741 ggctccttac ggggtgtcgg ggcgaagccc tgaccagatg gcaattgtaa tagcgtcgcg
    37801 tgtgacggta ttacaattgc acatcctgtc ccgtttttca ggccattata gcctgttatt
    37861 tttgtgcatc atagtcttat gcagacggca gaggcagggc gcagccctgc caggagtcag
    37921 tttttgatga taaaatcaga cttcaacatg aaacggagag aaagtgccaa tggcttctaa
    37981 aaaattttac tcagatgatg acatccagct ggcaaaagct gctttatcgg aattgccaga
    38041 tctcaccgct caacggaaaa cattgcgtga ttttcttgat gccattcgtg atgacattat
    38101 tgttctggta aggacaaagg gttatacact ggcagatgtt cgtgacacgc tcaaaaatgc
    38161 agggtatgaa gtaggagaga aggcacttcg tgacattatt cgggaagcag aatcgaaaaa
    38221 gccatcccgc cgttcttcct ctaaaacagc atctaaaaag acagattcag ccaggaaaga
    38281 tggcatcgat atgaacaaca attgatgtga caatgtcaca tcatgttctg atattatgat
    38341 gattacagaa aaaaagcccc ggcataaata ccagggcttt tctgtttaat cagactcgtg
    38401 gcggcaatga accaggataa tgccgtgcaa taatatcatt cagtttttca acaaggtcag
    38461 gacgcttaaa gacaatgtgt acggtccctt tctggtagcc acgcaccgta aaatattccc
    38521 cttctaacag cttaccgaca ttgtcgccgt tcccgataaa ttcaccgtac tgcgccccga
    38581 tgctgctccg gaagtcagga acgttgcgcc cctccagcag ataaaacgga cgtaccaggt
    38641 catcctgtgc ctcccgtccg ctgcagtcca gcgtaacacg cccgttactc cagcggtaca
    38701 gcaggttttc aataatgatt ctcttgccca gacggcaggg attattggtt ctgtaatccc
    38761 atgacaattt ccggtagaca tcaatcagtc cctgttcaaa tgtctgcatc ttactggcat
    38821 tcagatggcg aaaagttgcc agtacattgt ccagggtgat ttcaggacag gtgtcgctca
    38881 tcagctggct gttccactca tcacgctgac atgaactcat gaaggtgtac atccccgtat
    38941 ccgtcatcag gcggtcccag attttccggt cggcaagacg ggtgatattc cttttcgctt
    39001 cttccggttt atccatgcac cgaacggagt cggtcacgca gtcttcaaaa ccgtaaagaa
    39061 aaatatcacc tgacgcatca agcagaatcc tgcgcgctgt cgccaggcat tccatcgcct
    39121 gcatgaacag tgcaatacct tcgttacgct gcgcgatgat gcggtcgatg gccacagacg
    39181 gaataacatc tgtcgtgccg gcagtattca ggtcagcagt gggtaaagtc tgattcatca
    39241 cattgtctcc tcatcgtcac gactgccggg tcagccaggt catttcccgg aggagcgaag
    39301 ctccttctgc cgtccgggtc ttcggggatt tccccgcagt ggacctttcc gacgggggac
    39361 gctcaccacg tcacggggca gttgtccggg ggcagacgca aaagtttttg acgcctgcgg
    39421 caaaaagttt tgtggctggt acatctctcc cctggacgac tgaaacggga tgtggtcgtg
    39481 tatgtacccc cggaaagtcc atctgcggag aacaggccgc agtgcggcgg cagacaggca
    39541 gagacagctg cgccatcagg cgcagtggcc ttacacaccg taaggtgtgc agggagcgcc
    39601 gcatccggcg cgactgaaga aaaacgacgg cgcaacaatg gcatgtggtg gtcactggca
    39661 caccactccc gcacaggtga aaacagagaa aacagcacga cacctacaat caaaaaaagt
    39721 gaaattcctt cactttttca cacttatttt tcgcgctgat ttggtggtta tggtgataaa
    39781 ataaagtgaa gttttttcac tttttcaaag gtgttttatg accggctggg aactgcgaat
    39841 ctggcgaaaa agcatgctct ggtcacgcga aaaagcggcc agagaatttg gcgtgacaca
    39901 gagaacatgg cacgcctggg aaaatgcgga acaggtggat gtcaccgtct ggaggaccac
    39961 ccaggcactt tcagtccgtg acctgctgcc tcacatgcag gggatgcgca aggcagatat
    40021 catcaggcgt ctggaaaatg aactggggga aacggcagag gatgtgtgag cgtggttcac
    40081 agcctggcag aatgccaggc tactgctcat caggaaacag ttttttcagt tcttcaccgg
    40141 aaagtcccgt ggcctcctga atccgttccg gcgtccagcc catatcccga agccggcagg
    40201 ctatttcccg cgccacttca agagcacctt tttcaaaacc tttctgtata agacgttctg
    40261 caatcgtcat actgttttct ctgctcctgc tggcaaaagg cccggcattt tgccgggcct
    40321 tttggctctc atcagtgagt gatgttcttc agttcagctt cagaaagtcc ggtcagctga
    40381 cagatttgtg ctgccggcat tcccgcgttc cggagctccc gggcgatttc ccgtgctttt
    40441 tgaagcgccc cctgctgaac tccctgctgg ataccctgct ggataccctg ctggatacct
    40501 tgctggatac cttcctgctt aagacgttct gccagtgtca taatgctgtc ctcgtgctgc
    40561 ggcagtcgct gcgccagcgt ctgcaaaaat tcaaccgtta catgttcgct gccattaagc
    40621 agatagttga aaagaactgt aatctgctct tcatcctgga tgtcctgcag tgctctgacc
    40681 aggttgtcca gacacatcag catatcccgc tgacggatat gcttcatcag cagcgtcagt
    40741 gcggccatcc ggcgatggtt catgatttca ttgtcatcaa cgacagtgat atcaaccagc
    40801 ggaaacggct ttgtgtatat tttagccgca agcgccggat tctcaaaaca gtccagccag
    40861 ttcatgctgt acgggtacgg gctgcgctca ccgtgataga acagcaccgg aatgaccagt
    40921 ggcaacgttt tgtgctgctc cagatgtctc tgcatcgccg ccacactgta gcgtaacatg
    40981 cgaaaaggca tgtacagatt ttcggtgctc tggtgctcga tgagcgtata aacataacca
    41041 tcttcacccc cggtggtttt cacgctccag aggatatcgc tggcgtactg atgcaggtcc
    41101 ggctcaacaa aggtggcggg ttccagcttc agcgtggaca ggtcgcacaa ctgccggtac
    41161 tccgcaggaa gatgcagttc cagaaaatct ctggcgacgt cgggattcgc caggaacgac
    41221 cggaacgcgg catcatgcgg cgtgggcgtt gtggtgtttt tcttcttact cacggttcct
    41281 cctggtgaga tgtctgaacg tatcctgccg cctgcacagg cagacggcaa gggttaatca
    41341 gtggcagacc cgctcaattt caagcgcgcg gagcaggtgg ccccattcgc tgactttcat
    41401 agcggggata cgcacccgct tctgctcact gcagcgtctg gcgatatggc cgctcatcgc
    41461 caggacgtcc gggttaatct catcctgtgc cagctcctga cgtctgaagc gggtatggcg
    41521 gtgcaggggt ttattctccc gcaggcactc ttcacgcaga cgctttacat actcggcggt
    41581 tgcgaagata attgatggtc tcacaatatt actccttatg ttgttgatga ctgccgctct
    41641 tcctggcggg gaagtaacgg cggagagagg cagggtttat cgccctgcct ttttttcatt
    41701 tcggacagcg acgcagtgcc agcgcgagcg ctttcatctt ccagccttcc ggcgtgcggt
    41761 acatcacctt ccagtagccg tcgatgaaca cataatggaa gcgcacctct tcagtgcttt
    41821 cagtggcaat agcctccctg aaagaacggt actcacgcgc ctcactgtcc atccggaaac
    41881 cactgtcacg tccgtaataa gccgtcacac cgtccaccgg gttctgaagc gtatgcccgg
    41941 cgggtttgtc acaacgggga ccgaggcaac ggatatcacc gtgttcaacc agggcttcgg
    42001 cctgctgacc ggtggcatag tgagtatgca ggatgcgacc gttccaggtc ggctccccgt
    42061 gcaggtggca gtaaacggta cggaacaccc cgtcgcggca aaaaacggaa atcgtgctgc
    42121 tgactgtcat gaaaacatct ccttctgctg aatcaggtcg ccgggccgac cggttgctcc
    42181 ccggaggggc gaaagcccct tctgccgtcc acttcttcgg ggtttctccc cgcagtggac
    42241 ctttccgatg gggcgtccgt caccacgtca cggggcagct gtccgggggc ggacgcaaaa
    42301 ttttttgccg ggtttatggc taaaaatttt gtggacggta tatcttcccc tggactgctg
    42361 aaacgggatg tggttactcc agcccccgga aagtccacag cgggaaacag gccgcagggg
    42421 tggcggcgga agggttgact gcgccatcag gcgcagttgc cttacacacc gtaaggtgtg
    42481 cagggagcgc cgtttccggc gcgacagggg cggcgcgacc ggaacggtcg ctgcaagcct
    42541 ggtttttcag acatcggacc gtagcggcag cccgaaaccg cgccagcggg ttcggcggag
    42601 actggcgggt gcgccagcag ctgcctgtcg gagtcgggcc tgacgaccgg atgccgccag
    42661 gcagccggga gccgaaggtg tccccgcctc cggaaatcag aacaacagaa acgacataac
    42721 agaaaaatgc ctcacaccat gacgggcagg aagggcagcg ccgcaggcgc ggccctcctg
    42781 catttcaggc aggcgtaagc ctgccagggt tttccggcgg cctggcaggc catacccccg
    42841 ccggagcggg ggagtaaatc accgacggaa cacatgaccg tcaataaagg tgaaggagtc
    42901 caggaataaa tcccgcgcat acgcctcata gtcaaaatag agcgccaccg tctccggcac
    42961 gtcagccagc aggccggtat cactggcgaa ctcaaccgca aaagcctcct cactgtcagc
    43021 ctcgccccac caggcatcgc ggaaggtgtc aaaatccgtc tcaccggtat catccaccca
    43081 gagacgataa gcctcttcgc agccttcatc ccgcgcctgg cggaagcctt caacataggc
    43141 ccagttgata tggcattctg aggccatatt ccccgggaat ccctcataat cctgaaacat
    43201 cagttcagga tcggcttcat cctggtgaag agagcggcag gcggcgaaaa agtcgcgttc
    43261 atcatcaaac gtggccaggt caaaccagcg gccagcgatg cttccacagt tgtatttgtg
    43321 ccaggttcca acgtatacag caggtgcaac aacagacatt cccgaatcct ccatagtcac
    43381 atgttctgcg acaccgggcg gtgccgtgcc tccataaaag ccgggcgata aggcggggat
    43441 gcacagggcg caagggaagc c
//
LOCUS       DAHBHC010000039        42537 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHBHC010000039 DAHBHC010000000
VERSION     DAHBHC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.039"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..723
                     /locus_tag="M2A35_002529"
     CDS             <1..723
                     /locus_tag="M2A35_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCB7198818.1"
                     /translation="LLNVPMEKATQFHEYSTGKAHADALKDAWTENHGAAGREWVKWL
                     AGHQQEAKDAVRACRERWRNLIPESYGEQVHRVGERFAVLEAALVLSGHVTGWDVQAC
                     RDAVQHNFNAWVKEFGTGNREFKQMVEQAEAFLSSFGFSRYLPYPNSDERDLPIKDLA
                     GYRKGSIRNEDDEFRFYTFPHVFEGEIAQGFNPSHFARALSAAGMLEAGGDRRYKKKA
                     LGRIGGKQHIFYVLMFQPEAED"
     gene            934..1359
                     /locus_tag="M2A35_002530"
     CDS             934..1359
                     /locus_tag="M2A35_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7198819.1"
                     /translation="MTAQISAYGRLVADPQLKTTSKGTQMAMASMAVPLPCNQTDDGT
                     ATMWSSVLAFGRQADALAKHHKGELVSVAGNMQVSRWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQTGQHDDPYGDGIPF"
     gene            1396..1893
                     /locus_tag="M2A35_002531"
     CDS             1396..1893
                     /locus_tag="M2A35_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HCB7198820.1"
                     /translation="MTKLTINRKPKGIYGTPQKTAQAAQQQNKTTSAHKVMPDNQNTQ
                     QKLTGATPRRRMTKQQRKNRRRVNRLIELWPELFSQESPKPLKVGIFDDLIQDIAARG
                     ISLGQGTLRATLARYVQTPRYYRALVAGGARYDLKGQPCDEVTPLQQTHAGKLLEEMN
                     GNSKK"
     gene            1874..2290
                     /locus_tag="M2A35_002532"
     CDS             1874..2290
                     /locus_tag="M2A35_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198821.1"
                     /translation="MEIVRNDHAHGRRAGDHIVDEIAKKQRVDIVVSVTVESAKRQNK
                     RINDHSDVHGVITRLRQTFVTREEMIEAIDQHTACISVRDIPEIIANYFMITRRLYRR
                     KDKSTVNRLLMSDIREYLQERGYVNPPEQHYGRVGG"
     gene            2335..2524
                     /locus_tag="M2A35_002533"
                     /pseudo
     CDS             2335..2524
                     /locus_tag="M2A35_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            2656..2862
                     /locus_tag="M2A35_002534"
     CDS             2656..2862
                     /locus_tag="M2A35_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198822.1"
                     /translation="MNCINDLNTGDVRGGSVHLDAQTVMRLKQYRIDHVNNHPDQPLP
                     GVAQIVRHAVNAWLNQNGFASGGE"
     gene            2959..5490
                     /locus_tag="M2A35_002535"
     CDS             2959..5490
                     /locus_tag="M2A35_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198823.1"
                     /translation="MPVVPTVSGRQVESRGVQSAGLQTFSQPGIGDAFVRAGAEAIDV
                     FGQAKQRANIALVQEASLKLSQTGSDLLNNPETGLLNLKGKNAIGKGHEYTQQFDAQI
                     EQLAMSLPDEQARNAFMQQAQQQRIQFATQAGRYEIGQINAYEEGQFQATLMNNGKNA
                     AALYGDNAAYVLANTQTFQQIEDYGIAHGWSDEQIRARKIEFKEATAKATAQNAIGAN
                     YLQVRQQNGELSDTAAGSRRAVADSGSSDRTRGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRRGIDTVSEIINRWAPPTDKNDTMSYIKAVCEQLG
                     VSADEPLDASNPDTLKALCAAIIHHENGSQPYSDQQLTDGVSAALGLSQLPTKNKRYT
                     GVAWFDALSESDQASVLRQTDALARQQQAEYKTMLDSRVRDATAAYMRGVEFPNPPVE
                     GEFIAAYGVREGNLRYTEFRNLQIAGQYIGSFRNMPTSSITAYVEQLRPDTGETGEGY
                     ASRAELFDRVSAAATKVITQRQNNPFSAAVEIGAYKPITSNNPDDITAEVANRFSSQE
                     SLRALGINAPILNSEEAAALAQQVRGTQNVDQTIKLLQSMGEKLPAPAIRQVASVIAP
                     NSAATAYSALLLGTPDNQYDNTRPSIPYSQFIGYKPTMNKYDVAKVILSGDQLLNPTK
                     AMKNAGITPVQLPSEDKMKDAFDEQVGNAFARNPQLRQISYSLFKAAYAGIAYQSGDA
                     STVRTSMPDTDIVADAVKYATGGIYNSFNGGDVVMPFGMDKSTFKDRYTTSAKQALKD
                     AGLNVNAVSNFTPVNIGNNRYRLVNGSGRWATDPRTNKAIVVRVE"
     gene            5490..7550
                     /locus_tag="M2A35_002536"
     CDS             5490..7550
                     /locus_tag="M2A35_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198824.1"
                     /translation="MSDVFSLAPEGQAWIDDKTVANPARPEDYDPTPFRGSGTALLSG
                     VAEGALGLAQSAVGFSKRLISDPAFTADVAPTVNIFREMFPDADKTLNDTYDTIGKQL
                     QDARSYVKPDAGSQGMAAEVLNELGKFVPAIGTTMFGGPLIGAATAFSSTYEQSYQDF
                     KGKGVDEATARNLATQQSLFNAVGMALPAAVGTTLATRIASGVAINTGFGGLNRYSVG
                     ATLEEKGYTEMAKQYRVFDGQAMLVDAVLGGVFGGVHHLTTHNNHDGTESNISAEEIK
                     STFGEHYQQSPDGNIESMVRDLSDSGVIELINEPAIKNSDGEILLNKGHVTVASGKEP
                     IIRININGLKDHEELLETLHHEALHLEQRHYPDWKARKLANAVLKSANKHSDYPEFAY
                     GMSQYYEYKKRGEYPETYPEWLYAEEVAAHAVNPSLKTSVSSWEKIISGFKAYIFRYS
                     GIQVGTVTPEQLRLLVSMRLRNMKLEAAVEKVFGIRARERIKPSDIDAAHILNEGLHY
                     DIESSPVLHTSNESINSHVDAMDEAYRQLNDGQPVNVGGMARGLDGPLRSDISDTYQE
                     QYHEIQKVFEENGVRYETSSEPISESPVPRAESAFSSAGEHRGDVSVDPDTGQVLSSN
                     SYDLMAARDMAVANPELTIAHPETGNPTKLSDLLAELDEQIKTVQNESKVYSVAAACF
                     LRNP"
     gene            7550..10306
                     /locus_tag="M2A35_002537"
     CDS             7550..10306
                     /locus_tag="M2A35_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198825.1"
                     /translation="MKQACVEAIAQTLGRQPKADELKNIEDRIKEAVQHVHRKNAKEG
                     KSGIPDAQTYMDAAELVRQRVVHDVYKKRQRVAQNAIAISKITDTLDANIPPDQQTPV
                     NLQQFIFAGRRSRDKADISVTSAEELAIGAYQDWSRQLSAELLKAGDDVRKFFEQSRA
                     LGEQRFRSVFDRQAAKSAQLQILKEIYGEDTGNPLAKKIAQIWKDVTGRVRHEMNDNG
                     FDIGLREDWHTPYVDDADLIRNAGREEWLASLPMAEQATARLSGRQPPIEFARQKWVD
                     DAYNTQDRSNYVNPDGSIMNDVEYRQALEAIFETKATDGANKIEPGTFMGDGGIKSRG
                     SQHRVMAFKDAQSHFAYMERYTQQPLVGVMMSHLQSSSRDLGVVKAFGPDAERNFSLV
                     LDRIYKRAVTGGKRKKEMEDEAKLVARMFNSMAGLNGVASSSVFSSAVGGLRNLMTSA
                     MLGTSVLTATSDQAIMRANAQALGFTRGGMRLSVNTIKNLFSGDAKKANAELGLLVDS
                     HAAVVSKMGGFDLSRGITGWFAEKTLKWSGLIAMDRANKASFGLLMYKNIGELTRKFK
                     TLDDIKGTDKTILANKGWSNEDWAIMAAAELRPMTTAGHMGMTPDAIYAVPDNVIADI
                     MADRIARIRAGSEKALAALGDLPPERLKRMKEAFDAEAEQTITRMIRNARAEAAQKLL
                     GITHGEMTNAVTTATGIDTYAHDDAGELMKSFMLFKTTPFAGFRQLVNRTRDLDTVPA
                     IKFLASYIGGTTLAGMFAIQMNSLLNGNDPLDMTKPTTWVQALLKGGSFGIYGDFIFQ
                     DHTQYGSSIGATMGGPVLSFAEQLTKLLITNPQKALQGEETSFGADALKTARMITPFA
                     NLWYAKAITNHLILQQLQEMANPGYNDRVRDRAQREFDITSWWEPGAIAPRRAPDFGK
                     AVGN"
     gene            10306..10620
                     /locus_tag="M2A35_002538"
     CDS             10306..10620
                     /locus_tag="M2A35_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198826.1"
                     /translation="MTRDKIEYIASLKYEAEKWRRIGGWGTLISLSALLIDSWFRHID
                     AASLLFESAVLGGFICCWGGNYDRASKHKRELDAICFALFGKDYESSDLDIIEFNKTE
                     IS"
     gene            complement(11571..11978)
                     /locus_tag="M2A35_002539"
     CDS             complement(11571..11978)
                     /locus_tag="M2A35_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198827.1"
                     /translation="MALRVIQCSESVCRVLASMPHCSWFLSNTVFDYVHPYVHVFLKR
                     SAITLVDDHGWPDIYSRLKKLDPTSITAYRVRSLFLLVADEFIGVILSGEGIVSGIPF
                     SMQICIVPNGGVMEASHISIMPTSVKYFFISRV"
     gene            12186..13010
                     /gene="dinD"
                     /locus_tag="M2A35_002540"
                     /pseudo
     CDS             12186..13010
                     /gene="dinD"
                     /locus_tag="M2A35_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            13302..13919
                     /locus_tag="M2A35_002541"
     CDS             13302..13919
                     /locus_tag="M2A35_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCB7198828.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(13916..15598)
                     /gene="ligB"
                     /locus_tag="M2A35_002542"
     CDS             complement(13916..15598)
                     /gene="ligB"
                     /locus_tag="M2A35_002542"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCB7198829.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVTEWKENAQIKKLGSWLAAQQITGFEP"
     gene            15856..16479
                     /gene="gmk"
                     /locus_tag="M2A35_002543"
     CDS             15856..16479
                     /gene="gmk"
                     /locus_tag="M2A35_002543"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCB7198830.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16534..16809
                     /gene="rpoZ"
                     /locus_tag="M2A35_002544"
     CDS             16534..16809
                     /gene="rpoZ"
                     /locus_tag="M2A35_002544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCB7198831.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            16828..18936
                     /gene="spoT"
                     /locus_tag="M2A35_002545"
     CDS             16828..18936
                     /gene="spoT"
                     /locus_tag="M2A35_002545"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCB7198832.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            18943..19632
                     /gene="trmH"
                     /locus_tag="M2A35_002546"
     CDS             18943..19632
                     /gene="trmH"
                     /locus_tag="M2A35_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCB7198833.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            19638..21719
                     /gene="recG"
                     /locus_tag="M2A35_002547"
     CDS             19638..21719
                     /gene="recG"
                     /locus_tag="M2A35_002547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCB7198834.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(21704..22570)
                     /locus_tag="M2A35_002548"
                     /pseudo
     CDS             complement(21704..22570)
                     /locus_tag="M2A35_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22573..23778)
                     /gene="gltS"
                     /locus_tag="M2A35_002549"
     CDS             complement(22573..23778)
                     /gene="gltS"
                     /locus_tag="M2A35_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCB7198835.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24058..25449
                     /gene="xanP"
                     /locus_tag="M2A35_002550"
     CDS             24058..25449
                     /gene="xanP"
                     /locus_tag="M2A35_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCB7198836.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            25570..27279
                     /locus_tag="M2A35_002551"
     CDS             25570..27279
                     /locus_tag="M2A35_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCB7198837.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(27332..29650)
                     /gene="yicI"
                     /locus_tag="M2A35_002552"
     CDS             complement(27332..29650)
                     /gene="yicI"
                     /locus_tag="M2A35_002552"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCB7198838.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(29660..31042)
                     /locus_tag="M2A35_002553"
     CDS             complement(29660..31042)
                     /locus_tag="M2A35_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCB7198839.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            31335..31429
                     /locus_tag="M2A35_002554"
     tRNA            31335..31429
                     /locus_tag="M2A35_002554"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31369..31371,aa:Sec,seq:tca)
     gene            32067..33251
                     /locus_tag="M2A35_002555"
     CDS             32067..33251
                     /locus_tag="M2A35_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCB7198840.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANSQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            33362..34285
                     /locus_tag="M2A35_002556"
     CDS             33362..34285
                     /locus_tag="M2A35_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCB7198841.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(34289..35107)
                     /gene="nlpA"
                     /locus_tag="M2A35_002557"
     CDS             complement(34289..35107)
                     /gene="nlpA"
                     /locus_tag="M2A35_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCB7198842.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            35329..35622
                     /locus_tag="M2A35_002558"
     CDS             35329..35622
                     /locus_tag="M2A35_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198843.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(35663..36853)
                     /gene="nepI"
                     /locus_tag="M2A35_002559"
     CDS             complement(35663..36853)
                     /gene="nepI"
                     /locus_tag="M2A35_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCB7198844.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(37064..37516)
                     /locus_tag="M2A35_002560"
     CDS             complement(37064..37516)
                     /locus_tag="M2A35_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCB7198845.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(37569..38903)
                     /gene="adeQ"
                     /locus_tag="M2A35_002561"
                     /gene_synonym="yicO"
     CDS             complement(37569..38903)
                     /gene="adeQ"
                     /locus_tag="M2A35_002561"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCB7198846.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            39078..40844
                     /gene="adeD"
                     /locus_tag="M2A35_002562"
     CDS             39078..40844
                     /gene="adeD"
                     /locus_tag="M2A35_002562"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCB7198847.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAEFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEVSARLAQSAPPYGNTIARQP
                     VSASDFALQFTLGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(40889..42280)
                     /gene="uhpT"
                     /locus_tag="M2A35_002563"
     CDS             complement(40889..42280)
                     /gene="uhpT"
                     /locus_tag="M2A35_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCB7198848.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT        11183 a        10772 c        10660 g         9922 t
ORIGIN      
        1 ctgcttaacg tgccgatgga aaaagccacg cagtttcacg aatacagcac cggaaaggcg
       61 catgcagacg cgttaaaaga tgcctggaca gaaaatcacg gggcggcagg tcgtgagtgg
      121 gttaaatggc tggcaggcca ccagcaggag gcaaaggacg cggtaagggc atgtcgcgag
      181 cggtggcgca acctgatacc ggagagctac ggcgagcagg ttcaccgtgt gggggaaaga
      241 ttcgccgtac tggaggccgc gcttgtgctt tccggtcatg ttacaggctg ggatgttcag
      301 gcgtgccgtg atgcagttca gcataacttt aatgcctggg tgaaggaatt cggcacgggc
      361 aacagggaat ttaaacagat ggttgaacag gctgaggcgt ttttgtcgtc gttcgggttc
      421 agtcgatacc ttccctatcc aaacagcgac gagcgtgatt taccgattaa agaccttgcc
      481 ggatacagaa aggggagtat cagaaacgaa gatgacgagt tccgttttta cacgttcccg
      541 catgtgtttg agggtgagat agcacaggga tttaatccgt ctcactttgc ccgcgcgttg
      601 agtgcagccg gaatgctgga agcgggaggc gaccgccgtt acaagaaaaa ggcgctcggc
      661 aggattggcg ggaagcagca tattttttac gtgctgatgt tccagcctga agcagaagat
      721 taacttccct gtgtgaggta aaaagttgcg ggttatgcgg gttatcctgt gcataagtta
      781 attaactgaa tgattaaaaa ggaaattaat aacccgcacg taacccgcaa aacggcagtt
      841 ataacccgca aatgtgcgat tgtaacccgc aaaatttcac tgtaagagtg taaacaagcg
      901 taactatcac gttaaaaccg gagaacaaac agcatgacgg cgcaaatatc agcttacggg
      961 cggctggtgg ctgacccgca gttaaagacc accagcaagg gcacacaaat ggcgatggcg
     1021 agtatggcgg tcccccttcc atgcaatcag acagatgacg gaacggcgac gatgtggtca
     1081 tccgtcctgg cgtttggcag acaggctgac gcactggcaa aacaccacaa aggcgaactg
     1141 gtgagcgtgg ccggtaacat gcaggtgagc cggtggacgg gccagaacgg cgaaacgagg
     1201 cagggatggc aggttatcgc agacagcgta atcagtgcgc gaacggcgcg accgggcggc
     1261 aaaaaaggcc agcaggggca ggctactgat gcactgaaca gggcaaagca acagacaggc
     1321 cagcacgatg atccgtacgg ggacgggata ccgttttaat tcacaacagc gaacagagta
     1381 attacagggg aaggcatgac aaagctgacc ataaacagaa aaccgaaggg catttacggc
     1441 acgccgcaga aaacagcaca ggcggcgcag cagcagaata aaaccacatc ggcgcataaa
     1501 gtgatgccag ataaccagaa cacacagcag aagctcacag gagcaacacc gcggcggcgt
     1561 atgaccaaac agcagcgtaa aaaccgcaga cgcgttaacc gtcttattga gctatggcct
     1621 gaattattca gccaggaatc acctaagccg cttaaggtgg ggatatttga cgacctgata
     1681 caggatattg ccgcgagagg tatttcactc ggacagggaa cactacgcgc aacgctggcg
     1741 cgttatgtgc agactccgcg ctattaccgc gccctggtag ctggtggcgc gcgttacgac
     1801 ctgaaaggcc agccgtgcga cgaggtaaca ccactacaac agacgcacgc aggaaaacta
     1861 ctggaggaga tgaatggaaa tagtaagaaa tgatcacgca catggtcgcc gtgcaggtga
     1921 tcacattgtg gatgaaattg cgaaaaaaca aagagttgac attgttgtca gtgtcacagt
     1981 cgagagcgcg aagcgccaaa ataagcgtat aaatgatcat tcagatgtac atggtgtgat
     2041 cactcgttta cgccagactt ttgtaactcg tgaagagatg attgaagcta ttgaccagca
     2101 tacagcatgt atcagcgtca gggatatacc ggagatcatt gctaactact tcatgatcac
     2161 cagacgcctt taccggagaa aagacaagag cacggttaac cgtctcctga tgtctgatat
     2221 tcgcgaatac ctgcaagaaa ggggctatgt gaatccacca gaacagcact acggaagagt
     2281 cggaggctaa ctaaaaccct gtaccgaaga gcggcggcgc gatggggtga agtaatgcag
     2341 ttggggaact gacaaggaac ggtattacat atcaatgcga cgactggaat gttccaggaa
     2401 ggcacagagg ccaccgaaga aaccggataa tttagaagac ctgaaacggt cgcttaaacg
     2461 tactgaaaaa gaaatgggga tagatgggat cgggaaatca gtatggcgta actaccagaa
     2521 gtaagcacag agccggagca atccggcttt tttttggctg aaatcaatca atctgttttt
     2581 gtgatagaaa tatatttatc taccactttt tcagatcaat aatgtgcata gtttagccag
     2641 taagaggaaa ttgctgtgaa ttgtattaac gacctgaaca caggcgatgt cagaggtggt
     2701 tccgttcatc tggatgcaca gaccgtgatg cgccttaagc agtacagaat cgaccatgtg
     2761 aacaatcacc ctgatcagcc attaccaggc gtggcgcaga ttgtcagaca tgccgtaaac
     2821 gcctggctta atcagaatgg ttttgcatcg gggggtgagt aatgaatcgc tggtacagca
     2881 tcaaagcaga gggacgccaa tgttatctaa ctcaggtttt atgaattctg actcccgatt
     2941 caaaatagga ggttactaat gccagtcgta ccaacagtat ccggacgtca ggttgagagc
     3001 cgtggagttc agtcagcagg cttacagacg ttttctcagc caggtattgg tgatgctttt
     3061 gttcgggcag gggcagaggc aattgatgtt tttggtcagg caaaacagcg tgccaatatt
     3121 gccttggttc aggaggcatc ccttaaactc agtcagacag gcagtgatct gctgaataat
     3181 cctgaaacag gattgcttaa cctgaaaggg aaaaatgcta ttggaaaagg acatgagtat
     3241 acacagcagt ttgatgctca gatcgaacaa ctggctatgt cgctgccgga tgaacaggct
     3301 cgtaatgctt tcatgcagca ggcgcagcag cagcgtattc agttcgctac gcaggccggg
     3361 cggtacgaga tagggcaaat aaatgcctac gaagaaggcc agtttcaggc gacactgatg
     3421 aataatggta aaaatgccgc agcattatat ggcgacaacg cagcatacgt attggctaat
     3481 acgcaaactt tccagcaaat tgaggattac ggcattgcgc atggctggag cgacgagcag
     3541 attcgggcca ggaaaatcga gtttaaagaa gcaacagcaa aagcaactgc tcaaaatgct
     3601 attggagcaa actatcttca ggtaagacag caaaacggcg agttaagcga tactgctgct
     3661 ggatctcgcc gtgctgtagc agatagtggc tcttccgatc gtacccgcgg tatacgcaac
     3721 aataaccccg gtaatcttga atacagcaaa actaatccgt gggttgggca gactggtgat
     3781 gatggtcgct ttgccaaatt cgaaacccct gaacacggta ttcgtgcatt agggcgaaac
     3841 ctgatgtcgt atcagcggcg gggtattgat accgtcagcg agataattaa tcgctgggca
     3901 ccgcctactg ataaaaatga cactatgtcg tatatcaaag cagtgtgcga acaacttggc
     3961 gtttctgctg atgagcctct cgatgcatca aatcctgata ccctgaaggc gctttgtgca
     4021 gccattatcc atcatgaaaa cggtagccag ccatacagtg atcagcagtt aactgatggt
     4081 gttagtgccg cgctggggct ttctcagcta ccgacaaaaa ataagcgtta taccggtgtg
     4141 gcctggttcg atgctttaag tgaatcagat caggccagcg tgttgcgaca gactgatgca
     4201 ctagccagac aacagcaggc tgaatataaa acgatgctcg acagccgggt tcgcgatgca
     4261 acggctgcgt atatgcgtgg cgttgaattt cctaacccac ctgttgaggg tgagttcatt
     4321 gctgcttatg gtgtcagaga aggaaacctg cgatataccg agttcagaaa tttgcagatc
     4381 gccggacagt atataggctc tttccgcaac atgccgacaa gcagcattac cgcatatgtt
     4441 gagcaattgc gcccggatac tggtgaaaca ggggaggggt atgcgtctcg tgcggagtta
     4501 ttcgaccggg tatcagcggc agccacgaag gtaataaccc agaggcagaa taatccgttt
     4561 agtgcagcgg tggaaatcgg agcctataag ccgatcacca gcaacaaccc tgacgacatc
     4621 actgccgagg tggctaaccg tttctcgtcg caggaaagct tacgcgcgtt gggaattaat
     4681 gccccaatcc tcaacagtga agaggcggct gcgctggcac agcaggtgcg cggcactcag
     4741 aacgttgacc agacaattaa actgttgcag agcatggggg aaaagctacc tgcgccagca
     4801 atacggcagg tggcatctgt aattgctccg aacagtgcag caacagccta ttctgcgctg
     4861 ctgctgggca cgccggataa tcagtacgat aacaccaggc cgtcgatccc atatagtcag
     4921 ttcatcgggt ataagccgac gatgaacaag tacgacgttg caaaggttat tctttccggt
     4981 gaccaactac tgaatccgac gaaggctatg aagaatgcgg gcataactcc ggtacagttg
     5041 ccgagcgaag ataagatgaa agacgctttc gacgaacagg taggtaatgc atttgcccgt
     5101 aacccgcagt tacgccagat cagttatagc cttttcaaag ccgcttacgc aggaatagct
     5161 tatcaatctg gtgatgcctc tacggtaaga accagtatgc ctgatacaga cattgtagct
     5221 gatgccgtga agtatgctac tggcggtata tacaatagtt ttaatggcgg cgacgttgta
     5281 atgccttttg gcatggataa atctactttc aaagatcgct ataccacatc tgcgaaacaa
     5341 gcactgaaag atgccggact taacgtaaat gcagtgtcaa atttcacgcc agttaatatc
     5401 ggcaataacc ggtatcggct tgtaaatggt agcggacgct gggcaacaga tcccagaacc
     5461 aataaagcta ttgtcgtgag ggttgaataa tgtctgatgt tttttctttg gctccagaag
     5521 gtcaggcatg gattgacgac aaaacagttg ccaacccagc aaggccagaa gactacgatc
     5581 cgactccctt ccggggttca ggtactgcac ttttgagcgg cgtggcggag ggggctctcg
     5641 gtctcgcaca gtctgccgtc ggattcagca agcgcctgat tagcgatccg gcatttaccg
     5701 cagacgtggc accgacagtt aatatttttc gggagatgtt ccctgatgct gacaaaacac
     5761 tgaatgatac atacgacacg atcggaaaac agttacagga tgcccgcagc tatgtgaagc
     5821 ctgatgcagg cagccaggga atggcggctg aggtattaaa tgagcttggt aaatttgttc
     5881 ctgcaatcgg aacaactatg tttggtggtc cgctcatcgg cgctgctaca gccttcagtt
     5941 ctacgtatga gcagtcctat caggatttta aagggaaggg agtagacgag gctacagcgc
     6001 gtaacctggc aacgcagcag agccttttca atgctgtggg tatggcctta cccgctgccg
     6061 tcggcaccac actggcaacg cgcattgcct caggtgtggc aatcaacacc ggattcggtg
     6121 gactgaaccg ttactcagta ggcgcaacac tggaggaaaa aggctacacg gagatggcaa
     6181 aacagtaccg ggtatttgac ggtcaggcga tgttagtcga tgccgtttta ggcggggtct
     6241 ttggtggcgt tcatcacctg actacccata ataaccatga tggtacagag tcgaacattt
     6301 ctgcagaaga aataaaatca acttttggtg aacattatca gcaatctcct gatggtaaca
     6361 ttgagtcaat ggttcgtgat ttatctgatt caggagtaat agaacttatt aatgaaccag
     6421 caataaaaaa tagtgatgga gaaatacttc tgaataaagg tcatgtgaca gtagcttccg
     6481 gaaaagaacc aataataaga ataaatatta atggattaaa agaccatgaa gaactgttag
     6541 aaacccttca tcacgaggcg cttcatcttg agcagcgcca ttaccctgac tggaaggcca
     6601 gaaagctagc taatgctgta cttaaatctg caaacaagca ttctgactat cctgaatttg
     6661 catatgggat gtcacaatat tatgaatata agaaaagggg tgagtaccct gaaacatatc
     6721 ctgagtggtt gtacgctgaa gaagttgccg cacatgcagt aaatccatct ctaaagacaa
     6781 gcgtttcatc atgggaaaaa attatttcag gttttaaagc ctatatattc agatacagtg
     6841 gtattcaggt tggaaccgtt acacctgagc agttacggtt actcgtaagt atgcgtcttc
     6901 gcaatatgaa actggaagcc gcagtagaaa aagtttttgg tatccgggcg agggaaagaa
     6961 taaaaccaag cgatattgat gcagcccaca ttttgaatga ggggcttcat tacgatattg
     7021 aatcgtcacc agtgcttcat accagcaatg agagcatcaa cagccatgtt gatgccatgg
     7081 atgaagcata caggcaactt aatgatggtc agcctgtaaa tgttgggggg atggcgcgag
     7141 ggcttgatgg tccattgcga tcagatatat ctgatactta ccaagagcaa taccatgaaa
     7201 tacaaaaggt ttttgaagaa aatggtgtca gatatgaaac atcatcagag ccaattagtg
     7261 aatcgcctgt ccctcgtgct gaaagtgctt tctcatcggc tggtgaacac agaggggacg
     7321 tcagcgttga tccagataca gggcaggttc tctcatccaa cagctatgac cttatggctg
     7381 ccagggacat ggctgttgcc aatcctgagc tgactatagc tcacccggaa acaggaaacc
     7441 cgacaaaact ttctgatctc ctggctgagt tggatgaaca gattaagaca gtccagaacg
     7501 aatcgaaggt ttattctgta gcagcagcgt gcttcttgag gaacccataa tgaaacaggc
     7561 atgtgtggaa gctattgcgc agacacttgg tcgccagcca aaggctgatg agctgaaaaa
     7621 tattgaagat cgtatcaaag aggctgtgca gcatgtgcac agaaagaatg ccaaagaagg
     7681 caaaagtggt attcctgatg ctcaaacata catggatgca gcagaactgg tacgccagcg
     7741 tgtggttcat gatgtgtaca agaaaagaca gcgtgttgcc cagaatgcga tcgccatcag
     7801 taaaattact gacactctcg atgctaacat cccgcctgat cagcagacac ctgttaattt
     7861 gcagcagttt atttttgctg gccgacgatc gagagacaaa gctgatatct ccgttacttc
     7921 tgctgaagag cttgcaattg gggcatatca ggactggtcc cgtcagctca gtgctgaact
     7981 tctaaaagca ggtgatgacg tccgcaaatt cttcgagcag agcagggcgc ttggagagca
     8041 gcggtttcgg agtgtattcg acaggcaggc ggcaaagtct gcacaactcc agatcctgaa
     8101 agaaatatat ggcgaagata ccgggaatcc cctggcgaag aaaattgcac agatttggaa
     8161 ggatgtaaca ggccgtgttc gtcatgagat gaacgacaat ggctttgaca taggcctgcg
     8221 agaagactgg catacgccat atgtggatga tgctgacctt attcgcaatg ccgggcgcga
     8281 agaatggctg gcatcgttac caatggcaga gcaagctaca gcacgtcttt caggtcgcca
     8341 gccacctatt gagtttgccc gccagaaatg ggttgatgac gcttacaaca cgcaggatcg
     8401 cagcaattac gttaatcctg acggcagcat tatgaatgac gtcgagtacc gtcaggcact
     8461 ggaagcgatc tttgaaacga aggccactga tggggcaaac aaaattgagc ccggtacatt
     8521 tatgggagac ggaggaataa agagccgtgg ttctcagcac agggtgatgg cgttcaagga
     8581 tgcgcagtca cacttcgcct atatggagcg atacactcag cagccgctgg tgggggtgat
     8641 gatgtcgcac ctgcaatcat catccaggga tttgggcgtc gttaaagcct ttggtccgga
     8701 cgccgaaaga aacttttccc tggtactgga tcgcatctat aagcgcgcag taacaggcgg
     8761 gaaacgcaaa aaagaaatgg aggatgaggc caaattagtt gcccgtatgt tcaactcaat
     8821 ggctggccta aatggtgtcg cttcatcgag tgtgttctct tcagcagttg gtggtctgcg
     8881 taacctgatg accagcgcca tgcttggcac cagcgtactg acagcaacca gcgaccaggc
     8941 aattatgcgg gcaaatgcgc aggcgctggg ctttacccgt ggtggaatgc ggctttccgt
     9001 taacactatc aaaaacctgt ttagtggaga tgccaaaaag gcgaatgctg aactcggtct
     9061 gctggtggat tcacatgctg ctgtggtttc gaaaatgggc gggtttgact tgtctcgcgg
     9121 cataactggc tggttcgcag agaagacact gaagtggtcc gggctgatcg ctatggaccg
     9181 cgcaaacaaa gcgtcgtttg gtctcctgat gtacaaaaac attggcgagc tgaccagaaa
     9241 atttaagacg ctggacgata taaaaggaac cgataaaacc attctggcta acaagggatg
     9301 gagcaatgag gactgggcca tcatggcagc ggcagaactc aggccgatga ctactgccgg
     9361 gcatatgggg atgacgccag acgcgattta cgctgtgccc gataacgtaa tcgccgacat
     9421 tatggcagac cgtattgccc ggattcgtgc cgggagtgaa aaggcactgg cagcccttgg
     9481 agatctccct ccagaacgcc tgaaacggat gaaagaagca ttcgacgcag aggctgagca
     9541 gaccatcacg cgcatgattc gtaatgcacg cgctgaagca gcccagaaat tgctgggaat
     9601 tacgcacggt gagatgacca atgctgtgac caccgctacg ggtattgata catatgccca
     9661 tgacgatgct ggcgagttaa tgaagagctt catgctcttc aaaacaaccc catttgctgg
     9721 atttcggcaa cttgttaaca gaacacggga tttggacacc gttccggcca ttaaatttct
     9781 ggcctcatat atcgggggaa caactcttgc tggtatgttt gcgatccaga tgaacagttt
     9841 gttaaatggt aatgatccgc tggatatgac aaaaccaacc acatgggttc aggctttact
     9901 gaaaggtggt tcgttcggta tctacggtga ttttattttc caggaccata cgcaatatgg
     9961 ctccagcatc ggtgccacta tgggtggccc tgtgttgagc tttgcagagc agttaacaaa
    10021 attactgatt actaacccac agaaggctct acagggtgaa gaaacatcat tcggtgccga
    10081 tgcactgaaa acagcccgca tgatcacccc attcgcaaac ctgtggtatg cgaaagcgat
    10141 aaccaatcac ctgatattgc aacaacttca ggagatggca aaccctggtt acaatgatcg
    10201 agtaagggac cgcgcgcaac gggagttcga cataacgagt tggtgggaac cgggtgctat
    10261 tgcgccacgt agagcaccgg attttggaaa ggcggtgggg aactgatgac gagagataaa
    10321 attgaatata ttgcttctct taaatatgaa gctgagaaat ggagaaggat tggcgggtgg
    10381 ggaacattaa tatcactctc tgctttactt atagactcct ggttcagaca tattgatgca
    10441 gcatcattac tttttgaatc agcagtactt ggtggattca tttgctgttg gggtggtaat
    10501 tatgatcggg cgtccaaaca taagagagaa cttgacgcga tatgttttgc tttattcggt
    10561 aaggattacg aaagttctga ccttgatata attgaattca acaaaacgga aatatcgtga
    10621 catgtcacag gccgctttcg cggccttgtt tttaacgaat gccaccgccg cccgggcggg
    10681 aaaccgcaga taaaaaagcc cgctgcgcgg ttttattaat acttatacca ttaggattga
    10741 tccttcaatg gacaatcctt ccatcgtgtg gccagccatt catattcttt gtcttttctt
    10801 cagtatcggc tttgcatctt ctaccccctg aaagatgtcg ttttatggtg tatctgatga
    10861 ttcattccct aacaatagat attcgaaaaa ttaatcagtt aaaacaatta tttggataat
    10921 ttcaggtaaa aactatctta atcttctggt ggatctcaat gcgccgtgac ggtatcggga
    10981 ttaagggcgt ggatattcgc gatatatgcc gctacacccc tcaacagtaa tagatatgcg
    11041 cttttcactc tctcctttca ctgctgagaa cggcaacaac gtgccacagg aacgcgctgt
    11101 aatcgattct gacgcactta attgcgttgg gtagtttgcc agcctgtggg ggtaactctg
    11161 ttttatgagg ctttggctat agtgcaaaaa tgcactttgt cttgcctctc aatttgatga
    11221 gactggaacg acaacaggac agaccaccag caacagttac cgtattcagc cgttctgtgg
    11281 caccagtgga actatccacc cctgctttca gcaatgtgtt tgcgggttat tttggggcgt
    11341 ttgcgggtta tttgggatgt tttgcgggtt atgcgatgct tgaataatga acagtgcatt
    11401 ttgcaacata ttgatactaa agaacaaaaa atagttagca cgctaagata acccggtaac
    11461 ccgcataacc cgcactgttt tgtatatata tacgaaaaat tgcattcagg agggggggat
    11521 cgaaatccct acaatccatt gcttatggga ccgcccgcct cgtcaaattt ttacacccgc
    11581 gaaataaaaa aatacttcac gctggtgggc atgatgctga tatgggaggc ttccataacg
    11641 ccaccattcg gaactatgca aatctgcata gaaaagggga tgcctgatac aataccctcg
    11701 cccgataaaa tcacaccgat aaactcatct gccacgagta gaaagagcga acgaacccga
    11761 tacgcagtaa tgctggtcgg gtccagcttt ttaaggcggc tgtagatatc aggccatccg
    11821 tgatcatcca ccagcgtaat tgctgaacgc tttaaaaaaa cgtgtacata agggtgtaca
    11881 taatcaaaaa ccgtattaga aagaaaccag ctacaatgcg gcattgaagc caacactcta
    11941 caaacagatt cagaacattg aataactcgt aacgccaatt cttacttttc agccttcaca
    12001 aatgccgccc actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc
    12061 gtgcctgctc cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc
    12121 acaactgtat ataaatacag ttacagattt actttctttg caatcgatat cacatggagt
    12181 gggcaatgaa cgaacatcat caaccttttg aagaaatacg gcattatggc actgaagggc
    12241 aagaattttg gtcagctaga gaattggcac cgttactgga ttatcgggac tggcgcaatt
    12301 ttcaaaaggt actggcccgc gcgactcaag cgtgcgaagc cagcaatcaa gctgcatctg
    12361 accatttcgt tgaaaccacc aaaatggttg tattaggttc aggggctcaa cgagaacttg
    12421 aagatgttca tctctcccgc tatgcctgtt acctggtagt acaaaacggc gaccctgcga
    12481 aaccggtcat tgcggcaggg cagacttatt ttgctatcca gacccgacgg caggagcttg
    12541 ctgacgatga agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg
    12601 agttgaaaga acataacaaa caattggttg aggcagcaca gtaagctggg gtagcgacag
    12661 ctactgattt cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc
    12721 agaaagccat ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg
    12781 gttcaacaga actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc
    12841 gggatggcgt gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg
    12901 tgaggcaaac cattcaggaa cttggcggaa ccatgcctga ggagttgccg accccgcagg
    12961 tcagcatcaa gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaactaa
    13021 aaactctttt tgttgattga gatacccgat gcgtaagcca aagtccaggt gcggtgaaca
    13081 caatggctaa acattgcacc tttcttttcc acccctcaat tttaacctat tttttcttat
    13141 gcattttctc agacaagaag tcagaagaat gcatctacgc tacagaaaat agcgatttca
    13201 cataactaca agttatcaat ttcctctcac cttaaaaaat ctcaatcgtg acaatacgca
    13261 caaatcgcta ccctgccaga cagattttta gggagagaac catgctgtta cacattttgt
    13321 atttggttgg cattactgcc gaagccatga cgggggctct ggcggccggg cgacgccgca
    13381 tggatacatt tggcgtaatt attattgcga ccgccaccgc aattggcgga gggtcagtgc
    13441 gcgatattct gctgggccac tatccgctcg gctgggtcaa acacccggaa tatgtgatta
    13501 tcgtcgctac cgccgcagta ctcaccacta tcgttgctcc tgtaatgcct tacctgcgca
    13561 aagtgtttct ggtgctcgat gcgcttggac tggtggtctt ttctatcatc ggcgcacagg
    13621 ttgcactgga tatggggcac ggcccaatta ttgccgttgt cgcggcggta accaccggcg
    13681 tgtttggcgg cgttttgcgc gatatgttct gtaaacgtat cccactggta ttccagaaag
    13741 agttgtatgc cggtgtctca tttgcctccg ccgtgctgta catcgcgctg caacactatg
    13801 tttctaacca tgatgtggta attatctcca ccctggtatt cggctttttt gcccgcttac
    13861 tggcgctacg ccttaagctg ggattaccgg tcttttacta cagccacgaa ggacactaag
    13921 gttcaaaacc tgtgatctgc tgggcagcca gccaactgcc cagcttcttg atttgcgcat
    13981 tttccttcca ttcagtaacc tgtctggcgc gccccgatcc agtccccggc agctgctgcc
    14041 agaactgctc cgtgctaaat aaaagttgcg accaggaccg ttcatcactg gcattaagcg
    14101 ccgcccgggt tagcggtatt cccattgcca tcacccagcg agtaaaaggc tgcttacgag
    14161 ccagattaaa ccgatgccat agctgcgcac ttttactttt cgcgatcccc ggcgtgttct
    14221 gtaattgctc tggcgttaat aaaagccagg aaaagatatg ctcaaagcga tgagtctgat
    14281 gcagcgcgcg ccagccggcc tcaccaatac catccagccc cagaacctgt tttgacccca
    14341 gccagactaa gcgtgaaatg aactgttcct gacaaacatc agaagcaaag tagcaggtca
    14401 acgagttaaa gcggttttct ggtggtgtcg gttttgtacg ttctgcaccg cgccagacca
    14461 catcatcaat gcgaggaata ccctgaccgg caaggctgac gaggatctga tcaccaggcg
    14521 caatatccca ctcctgccag cgcctgacgg aaccaatatt cacccgctgg acttttttat
    14581 catccagcat gacaggtgcg agtgacgcaa ccaccgatat tttaccgctc ttacccaccg
    14641 caaactgaat cgccttcact tcggcaacct gagctacagg ttgatatttc caggccacca
    14701 gccattctgc ctggcctggt agccaatggc gggattcggg ttctttcgcc cctcgcacaa
    14761 ccacgccatc ggtgacgaag ggtaatttcg ctttccacca ctcattgcgt acgcgcgcaa
    14821 cctcatcagc atttttaacc gcacgggtat acctctgcgt tagagtaaaa cctgcggtag
    14881 ccagctcctt taaacgatcg gtcattaact gcggtccatc cggccatgcc cagacaaaaa
    14941 cgcccagaga attcagcgtg tcgctatcgt cctggcgcat catcaggcca gcaacttttg
    15001 cgcgggcatt tattcccccc atttgttgtt ggatatgccc ctcgcgctgg agaaatattt
    15061 ccccctgaag cgtactgttg gctaaaggcc cgctaacggt ttgcggcaca gcggaaatta
    15121 agctaacttt ctgcgtccag tcctcgcctt tcaggccatt accgcgactg attgctttgt
    15181 tcagtttccc gtcccgataa accagggtta ccgcaacgcc atcaactttt ggctgcaccc
    15241 aaagatcgct acgttctcgc atccacagac ttaatgcgtt tttatccgcc attttgcgta
    15301 cacccgtatg ggcaaccgga tgcataaccg caccatttaa aggcggcatc atcacgtcgc
    15361 ggggctcgct cacaaagcag cgttgccact gcgtaagacg ggcgcttaac tgatcgtaaa
    15421 caccgtcttc cacctcactt tttccttcct tccagtagtc atcgtcccac tgttttattt
    15481 gctgttgcag gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg
    15541 cccacacaga tgattgccag cacaagatac ttattaatat cgccatccat actttcatct
    15601 tcacctccgc tgtagtcagg ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc
    15661 aaaaacggag tctgcgagga cgcttcctga gaatcgtctt tattgcagta aatcacaggc
    15721 aaatgcggaa acagctacgc aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct
    15781 acgtcacatg gccgcgccgt gtataataag ctcgtatgta ggctttattt cgctaatcac
    15841 atacgaaaga tactcatggc tcaaggcacg ctttatattg tttctgcccc cagtggcgcg
    15901 ggtaaatcca gcctgattca ggctttatta aaaacccaac cgttgtatga cacccaggtt
    15961 tctgtttcac acaccacacg ccaaccgcgt cctggtgaag tccacggtga acattatttc
    16021 tttgttaatc atgatgaatt taaagaaatg attagcagag atgcgttcct cgaacacgca
    16081 gaagtttttg gtaattacta tggcacttcg cgtgaggcca ttgagcaagt actggcgacc
    16141 ggtgtcgatg tttttctcga tatcgactgg cagggcgcgc agcaaattcg ccagaagatg
    16201 ccgcacgcgc ggagtatctt tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta
    16261 cgcggtcgcg gtcaggacag cgaagaggtc attgcaaagc gtatggcgca agctgttgca
    16321 gaaatgagcc attacgccga atatgattat ctgattgtga atgatgactt cgataccgcg
    16381 ttgaccgatt tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag
    16441 cgtcatgacg ctttaatcag caaattgttg gcagactgaa cctgatttca gtatcatgcc
    16501 cagtcatttc ttcacctgtg gagcttttta agtatggcac gcgtaactgt tcaggacgct
    16561 gtagagaaaa ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag
    16621 atgcaggtag gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc
    16681 gcgctgcgcg aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc
    16741 caggaacagc aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt
    16801 cgtcgttaat cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt
    16861 caaacctacc tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt
    16921 gatgctcacg aggggcaaac acgttcaagc ggtgaaccct atatcacgca cccggtagcg
    16981 gttgcctgca ttctggccga gatgaaactc gactatgaaa cgctgatggc ggcgctgctg
    17041 catgacgtga ttgaagatac tcccgccacc taccaggata tggaacagct ttttggtaaa
    17101 agcgtcgccg agctggtaga gggggtgtcg aaacttgata aactcaagtt ccgcgataag
    17161 aaagaggcgc aggccgaaaa ctttcgcaag atgattatgg cgatggtgca ggatatccgc
    17221 gtcatcctca tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc
    17281 ccggacaaac gtcgccgcat cgcccgtgaa actctcgaaa tttatagccc gctggcgcac
    17341 cgtttaggta tccaccacat taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat
    17401 cccaatcgtt accgcgtaat taaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag
    17461 atgatccaaa aaatcctctc tgaaatcgaa gggcgtttgc aggaagcggg aataccgtgc
    17521 cgcgtcagtg gtcgcgaaaa gcatctttat tcgatttact gcaaaatggt gctcaaagag
    17581 cagcgttttc actcaatcat ggacatctac gctttccgcg tgatcgtcaa tgattctgac
    17641 acctgttatc gcgtgctggg ccagatgcac agcctgtaca agccgcgtcc gggtcgcgtg
    17701 aaagactata tcgccattcc taaagcgaac ggctatcagt cgttgcacac ctcgatgatt
    17761 ggcccgcacg gcgtgccggt tgaggtccag atccgtaccg aagatatgga tcagatggcg
    17821 gagatgggtg ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag tactaccgca
    17881 caaatccgcg cccagcgctg gatgcaaagc ctgctggagc tgcaacagag cgccggtagt
    17941 tcgtttgaat ttatcgagag cgttaaatcc gatctcttcc cggatgagat ttacgttttc
    18001 acaccggaag ggcgcattgt cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat
    18061 gcagtgcata ccgatatcgg ccatgcctgc gtgggcgcac gcgtcgaccg ccagccttac
    18121 ccgctgtcgc agccgcttac tagcggtcag accgttgaaa tcattaccgc tccgggcgct
    18181 cgcccgaatg ccgcttggct gaactttgtc gttagctcga aagcgcgcgc caaaattcgt
    18241 cagttgctga aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac
    18301 catgctttgg gtggtagccg taagctcaat gaaatcccgc aggaaaatat tcagcgcgag
    18361 ctggatcgca tgaagctggc aacgcttgac gatctgctgg cagaaatcgg acttggtaac
    18421 gcaatgagcg tggtggtcgc gaaaaatctg caacatgggg acgcctccat tccaccggca
    18481 acccaaagcc acggacatct gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg
    18541 aaatgctgcc gccctattcc tggcgacccg attatcgccc acgtcagccc cggtaaaggt
    18601 ctggtgatcc accatgaatc ctgccgtaat atccgtggct accagaaaga gccagagaag
    18661 tttatggctg tggaatggga taaagagacg gcgcaggagt tcatcaccga aatcaaggtg
    18721 gagatgttca atcatcaggg cgcgctggca aacctgacgg cggcaattaa caccacgact
    18781 tcgaatattc aaagtttgaa tacggaagag aaagatggtc gcgtctacag cgcctttatt
    18841 cgtctgaccg cccgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg
    18901 ccagacgtga ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc aacacgttat
    18961 gcacgcatct gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg catggagcag
    19021 gtccacaaac ctcataacgt ttctgcgatt attcgtaccg cagatgccgt tggcgtacat
    19081 gaagttcacg ccgtctggcc tggtagccgc atgcgcacca tggcttcggc agcggcgggt
    19141 agtaacagct gggtacaggt gaaaacacac cgcaccattg gcgatgccgt cgctcatctc
    19201 aaaggccagg gcatgcagat tctggcaacc catctttctg ataacgctgt cgatttccgc
    19261 gaaattgatt acactcgccc gacctgcatt ttgatgggac aggagaaaac gggcatcacg
    19321 caggaagcat tggccctggc ggatcaggac atcatcattc cgatgatcgg catggtgcag
    19381 tcgctgaatg tttccgttgc ctcagccctc attctttacg aagcccagcg tcagcggcaa
    19441 aatgcaggca tgtacctgcg tgaaaacagc atgttgccgg aagcagagca acaacgcctg
    19501 ttgtttgaag gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat
    19561 ccccacgtca atcagcaagg cgagatcgaa gctgatgccg actggtgggc tactatgcag
    19621 gctgcagggt aagtgccatg aaaggtcgcc tgttagatgc tgtcccactc agttccctaa
    19681 cgggcgttgg cgcagcactt agtaacaaac tggcgaaaat caacctgcat accgtgcagg
    19741 atctactctt acaccttccc ctgcgctacg aagatcgcac ccatctctac cccatcggag
    19801 aactactgcc gggcgtttat gccacggtgg aaggcgaagt gctgaactgc aatatctcct
    19861 tcggcggtcg gcggatgatg acctgccaga tcagcgacgg ttccggcatc ctcaccatgc
    19921 gctttttcaa tttcagcgcg gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg
    19981 cttatggcga agcaaagcgc ggtaaatatg gtgcggagat gatccacccg gaataccgcg
    20041 tgcagggcga tctcagcacg ccagaattac aggaaacgct cacgccggtt tatccaacaa
    20101 cggaaggcgt aaagcaggcc acgctgcgta aattaaccga ccaggcgctg gatctgctcg
    20161 acacctgcgc cattgaagaa ctcctgccgc cggaactgtc acaaggaatg atgacgctac
    20221 cggaagcgtt gcgcactttg caccgcccgc caccgacgct acagcttagc gatctggaaa
    20281 ccgggcagca tccggcgcaa cgtcgtctga ttctggaaga actgctggcg cacaacctca
    20341 gcatgttagc cttacgtgcc ggagcacagc gttttcatgc ccagccgctg agcgccaatg
    20401 acacgctgaa aaataaactc ctcgccgcct taccgttcaa gcaaacgggc gcacaggcac
    20461 gcgtagtggc ggagatcgag cgcgatatgg cgctggatgt gccgatgatg cgtctggtgc
    20521 agggcgatgt aggttccggt aaaacgctgg tcgccgccct cgccgcgttg cgtgcgattg
    20581 cccacggcaa acaggtagca ttgatggcac caaccgaatt actcgccgag cagcacgcca
    20641 ataacttccg caactggttt gcaccgctcg gtatcgaagt gggctggctc gccggtaagc
    20701 agaaaggtaa agcacggctg gcacagcagg aagccatcgc cagcggtcag gtgcagatga
    20761 ttgtcggtac acacgccatc ttccaggaac aggtgcagtt taacggcctg gcgctggtga
    20821 ttatcgacga acagcatcgt tttggcgtac atcagcgtct ggcattgtgg gagaaaggcc
    20881 agcagcaggg cttccatccg catcagttga tcatgaccgc cacgccgatc ccccgcacgc
    20941 tggcaatgac tgcgtatgcc gatctcgata cctcggtgat agatgagctg ccgccaggcc
    21001 gcacgccagt gactacggtc gctattcctg atacccgccg taccgacatc attgaccgcg
    21061 tgcgccacgc ctgcataact gaaggtcgtc aggcatactg ggtttgtacg ttgattgaag
    21121 agtcggaatt gctggaagcg caggcggcgg aagctacctg ggaagagttg aaactggcgc
    21181 taccagagtt gaacgttggc ctggtacacg ggcggatgaa acctgccgag aaacaggcgg
    21241 tgatggcgtc gtttaaacaa ggtgagctac acctgctggt tgccacaacc gttattgaag
    21301 tcggcgttga tgtgcctaac gccagtctga tgattatcga aaacccggag cgtctgggtc
    21361 tggcgcagtt acaccagctt cgcgggcgtg taggtcgtgg tgcggtggct tctcactgcg
    21421 tgctgctcta caaaacgccg ctctccaaaa ccgcgcaaat tcggctgcaa gtgctgcgcg
    21481 acagcaacga cggttttgtg attgcacaga aagacttaga gattcgcgga ccgggtgaac
    21541 tgttaggtac gcgtcagacg ggtaatgctg agtttaaagt ggcggattta ctgcgcgatc
    21601 aggcgatgat cccggaagtt cagcgcctgg cgcgacatat tcacgaacgt tacccacaac
    21661 aagcaaaagc cctgatagaa cgctggatgc cggagacgga acgttactcc aacgcatgac
    21721 cagactcatc acaagacaag tcacgcagta aatcttcaat ctggatagtg aggatatcca
    21781 ttgcctcata cgcatcctgt gcaattcgcg ccgccattgc gccttccggt tcaccacgca
    21841 gaatttcagc cgctgcctgt acgccagcca caatcatttc aggtgttaac atttggcgaa
    21901 gaaaagcgcg attatcagag gaccagccat ctttatggcc taatggatgc ccccagccta
    21961 tccattcttg cgatcataac ggattggctt ctatgggata aattagatgc gcggcatact
    22021 gaatagcaca caccgctttt cctggcgtat ccagcgccca ccagcgcgac cagagagaac
    22081 gaattaacgg cgcggcccca cccagagtat tgaacaccgc cagccagcaa agaacgttat
    22141 tgaaaccacg ctcgttatcc atacgctgca acgcggtgtc cagtaaaaat tgccgtacct
    22201 gctggcgttg ggaagcattc atcatctcct gccagagata gggccgacac aatgccttca
    22261 ttaaggattc ttcgaagtga tcaccataac cgccagctaa aacggtttca tgccattgcg
    22321 ccaggcagag cggaaacaga tagcgaaaca actctatttg catcggttcg ttataaacga
    22381 gattgagtac atagtaagca ctcaaatccc attcgctgat ctgctgccac tctttagccg
    22441 ccagcgcctt gagttcggca tctgcgtaat caaattccgt ttcccataca cagagcactg
    22501 gcggctcatc accaaaaata tggcgacagt gttcacgtag cgtttccggt tccttccgac
    22561 gctttttcat ttttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    22621 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    22681 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    22741 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    22801 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    22861 catcggcagc gccagcgaag ccagctccca cagtttcagc cccatcagcg ccatcgccag
    22921 gaacaacgac aggcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    22981 gcccatcatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    23041 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    23101 gatagcaatc agcgcgatag tttcaatcag caccagcgag gtgatcatgc gtcccacatc
    23161 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    23221 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    23281 cgttgcacag gccatcgcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    23341 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    23401 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    23461 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    23521 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    23581 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    23641 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    23701 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    23761 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    23821 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    23881 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    23941 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    24001 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcgatg
    24061 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    24121 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    24181 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    24241 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    24301 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    24361 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    24421 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    24481 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    24541 tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggttgggtt aacgtccatt
    24601 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    24661 gcaggcgtgg tcttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    24721 gtggcctcac tggtgattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    24781 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    24841 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    24901 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    24961 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    25021 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    25081 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    25141 ctgggtctgt tcccggcagt gagcggtttt gtacaacaca ttccagaacc ggttctgggc
    25201 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    25261 gagccgctga accgtcgggc gattctgatt atcgcgctgt cgctggcggt tggtctgggc
    25321 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    25381 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    25441 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    25501 ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    25561 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    25621 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    25681 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    25741 gcgccatctc atatcgtgct ggagaacgtc acgtttggtc gtgatggtca gcccgcgacc
    25801 ctggtggcaa aaagtgtcga cattgcgcta agcagtcggc aactgaccga accacgccat
    25861 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    25921 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    25981 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    26041 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    26101 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    26161 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    26221 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    26281 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcctggaa
    26341 gtgatcgatg ctcgtttgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    26401 cgcaacatga ccttcagtaa agatgactgg cagacacaag aaggcaaact gtcgatgaac
    26461 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    26521 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    26581 acgtcaggga actggctgcg tgacgggaaa acgttgatcc ttgatgatgc ggcaattgcc
    26641 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    26701 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    26761 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    26821 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    26881 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    26941 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgccagt
    27001 gtttcacaaa cgccacaacg tcagacacat atcagcctga atggacgcgg tgtgccggtg
    27061 aatattttgc aacagtgggg atggcctaaa ttaccgttga ctggcgacgg caatattcag
    27121 cttaccgcca gtggcgatat tcaggccaat gtcccgttga aacctacggt tagcgggcaa
    27181 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc tggcatcgtt
    27241 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    27301 actcagacgt acccggcatt ccatcaatag attacaacgt aattgtcagc gcattccctt
    27361 gaggcttcac caccagcccc tgctcacttt cagcctgcga accgtcttgc agaccattta
    27421 ctttcacaac attgcgcagg cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    27481 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagtagcgg
    27541 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    27601 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    27661 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    27721 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    27781 ccgcttcagt gaacaccggc gcgaccatca cgttgtcgcc taacatgtat tgacggtcaa
    27841 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    27901 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    27961 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    28021 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    28081 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    28141 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    28201 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    28261 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    28321 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    28381 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    28441 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    28501 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    28561 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    28621 cgcagatttt cagtcctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    28681 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    28741 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    28801 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgcgggcg
    28861 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    28921 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    28981 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    29041 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    29101 ggttccaggt ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac
    29161 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    29221 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    29281 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    29341 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    29401 gcggataatg aggaccgtta ttcagcgccc cctgaaaatg ctcaatccgc acaccgacaa
    29461 taccttcctg tggggagaaa aagcgcaacg taaataaagg cgtatcaagc tgccaggtac
    29521 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    29581 cgaacacctg aagcgggtga atcaaattga ggccaggttg aatcaaccag tttccatcgc
    29641 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    29701 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    29761 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    29821 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    29881 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    29941 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    30001 cgacggtatc ggacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    30061 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    30121 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    30181 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    30241 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    30301 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    30361 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    30421 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat gaatgccacc acggaaagga
    30481 ccgcgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    30541 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    30601 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    30661 tattgacgac ggtataaagt aaggtaagta gggtgtaagt aattgctgca tagatcattt
    30721 tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc ccgaatggca
    30781 gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt cgatcggcca
    30841 acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa aacatggttc
    30901 cgacaaaacc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata tataacatta
    30961 cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt ttctctttaa
    31021 cggacaacac ttcactcttc atctgctttt ccttgatgct ataggggtgc tgagaccgat
    31081 aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt tattaattta
    31141 tgattcttgt tttatgtgat cgtggtagcg ttaatttcgc tcatatatca ttgtaaaata
    31201 taggttttat atgaactata atgctttcgt gataatacgc tgcgtgtatt aggcggaaaa
    31261 aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc gcctccatgt
    31321 agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc aaatccagtt
    31381 ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccaa aatgcctctc
    31441 ctgacgttct ccaacccctt gtaatactgt caatccagat attttctact gctcaatatt
    31501 caatcggctt caggaatacc aggtctaagg ttttgtgatg gacgatgagc ggctgaagaa
    31561 tccgcctgtc ggttcttctg tcctcgaacg catccgcgat attcacgcca gtatgcgccg
    31621 tgtttatttg caggtgattt gcccccctgc tgactctcag ccaactgtat cgccattaag
    31681 gtgaagatgt tgttttactc gccccagaac accctttttt atacgactat tctatttctt
    31741 taaccgctgg cgaaaaaaaa ctgactataa acttcaccaa taatcagact gccatcaaga
    31801 ttaatttgtt catttaatat atcaaatcaa cttgttttat ttttactatc aaaaatagct
    31861 cattgcctta ctataattaa tatataaatt cggtaattaa ttcttaacat gcttttgcta
    31921 ataatctcaa ttgctggccc tataatattg cgctagcatt gctttctggt tgtatcagcg
    31981 atactcaaaa attcttaata caatactcat tcgactggta cttatttgta actcagttat
    32041 attttttcgc ccggtgattc agaagaatgc aaaaaacggc taccactcca tcaaaaatac
    32101 ttgatctcac tgccgcggca tttttacttg tcgcctttct gacgggtatt gcgggcgctc
    32161 ttcagactcc taccctaagt atattcctcg cagatgaact gaaagcccgt cctataatgg
    32221 taggtttttt cttcaccggt agcgctatta tgggaattct ggtcagtcaa tttctggcaa
    32281 ggcactccga taaacaaggc gaccgtaaat tactgattct gctatgttgc ttatttggag
    32341 tgctggcctg cacgcttttt gcgtggaatc gcaactactt cattctgctc tcaacgggcg
    32401 tacttctgag tagttttgct tctaccgcaa actcgcaaat gttcgccctc gcccgtgaac
    32461 acgccgacag aacaggccgt gagacggtta tgttcagtac atttttacgt gctcagatct
    32521 cgcttgcctg ggttatcggg ccaccgcttg cttatgaact ggcaatggga tttagtttta
    32581 aagtgatgta tctcaccgct gccatcgcat ttgttgtttg cggactgata gtctggttgt
    32641 ttttgccatc aatacaaaga aatattcctg tcgttaccca acccgtagaa attttaccct
    32701 ccacccacag gaagcgggat acgcggctac tttttgtggt ctgttcaatg atgtgggcgg
    32761 cgaataatct ctacatgata aatatgccgc tatttattat tgatgaactg catctaaccg
    32821 ataaactggc tggagaaatg attggtatcg ctgccggtct ggaaattccg atgatgttaa
    32881 tcgcaggcta ttacatgaaa cgtattggca agcggctatt aatgctcatt gctatcgtga
    32941 gtggaatgtg tttttacgcc agcgtactca tggcgacgac tccggcggtt gagctggaat
    33001 tgcaaattct taatgccatc ttccttggta ttctctgtgg tatcggcatg ctttattttc
    33061 aggacttgat gcctgaaaaa ataggctctg cgacaacgtt atatgcaaat acttcacgcg
    33121 tcggctggat tatcgccggc tctgttgacg gaattatggt tgaaatctgg agctaccatg
    33181 cgttgttctg gctggcgata gggatgttgg gtattgcgat gatttgcctg ctgtttatta
    33241 aagatattta gctcaccagt ctgggctaag aaaaatcggc gtggcgcaaa cagagccatc
    33301 ctcgccattt ttgctatcat gcctgcatac ataaacgaca aaacagtatg cagagggaaa
    33361 aatgggttcc accagaaagg ggatgctgaa cgttctgatt gccgccgtgt tgtggggaag
    33421 ttcaggggtc tgcgcgcaat acatcatgga gcaaagccag atgtcgtcgc agtttttgac
    33481 tatgacgcgt ttgatattcg ccggtttgat tctactgacg ctgtcatttg ttcatggcga
    33541 taaaatcttt tctattatta acaatcataa agatgccatt agcctgctga ttttttccgt
    33601 ggttggcgcg ctaactgtac agctcacttt tttgctaacc atcgaaaaat cgaacgcagc
    33661 cacggcaacg gtgctgcaat tcctctcacc gacgattatc gtcgcctggt tctcactggt
    33721 gcgtaaatcg cgcccgggca ttctggtttt ctgcgctatt ttgacatcgc tggtcgggac
    33781 ttttttattg gtgacacacg gtaatccgac gtcattatcg atctctcctg ccgcgttgtt
    33841 ctggggcatt gcctcggcat ttgctgctgc attctatacc acctatccct caacgctaat
    33901 tgcccgctat ggcacgttac cagtcgtcgg ctggagtatg ctgattggcg gtctgattct
    33961 gttgcctttt tatgccagac aaggaacaaa ctttgtcgtt aacggcagtt tgattctggc
    34021 gtttttttat ttggtggtca ttggtacgtc cctgacattt agtctgtacc tgaaaggagc
    34081 acaattaatt ggcggtccaa aagccagcat tttgagctgt gcagaaccat taagtagcgc
    34141 gctactctct ttgctgttgc tggggatcac gttcacatta ccggactggc tgggaacgct
    34201 gctgattctg tcatcggtga ttttgatttc aatggattcc cgtcgccgcg ccagaaaaat
    34261 aaatcgtccg gcgcggcatg agtgagaatt accagccagg caccgcgcca ccgttaaaaa
    34321 tggtttctgc cgctttagcg acttcgggtg attgataaga ttgcagaaat tccttcacgt
    34381 tttctgcatt cttattatct tcccgtgcca ccaaaatatt cacatacggc gaattcttat
    34441 cttcaataaa tacgctgtcg tgcaccggag aaagccctgt ctgctgaatg taagtggtac
    34501 tgataatcgc tacatcaact ttgggatcat ccagtacgcg cggaagctgc gccccctcga
    34561 gttccataat ctgcaaatga cgaggattat cggttatatc cagcgcggta ggtaataacc
    34621 cttttccctc tttcagagta attagcttct ctttttgcaa cagtaaaagt gcgcgaccaa
    34681 ggttggtcgg gtcgttgggg atcgccactg tcgcgccttc ttttatttgc gccacggttt
    34741 tgattttttt ggaataaccc gccataggga agacaaacgt atttcccacc gctaccagtt
    34801 tataaccatg cgcctgatta tcttgttcaa gaaagggtcg atgctggaag acattcgcat
    34861 caagttcgcc atgatttgtc gcatcgttcg gtagcaacga accgctaaat cctaccagtt
    34921 caacatcgag gccatatttc tcttttgcca cctttttagc gacttccgcg acatcttgtt
    34981 ctgcgccatt tataacgcca actttaatgt gttttgcatc gctgctactc tggtcgcaac
    35041 ctgccagcag aattccggcc agcaataatg cggcccctgt ccgtagatga tgtgttgtca
    35101 gtttcatttt tttatccttt tattaatgat tacctgacta tagcggtaag gtcgctgcgg
    35161 tttaaaaaac gaaacgctat cgataagaat aaaaaggaat aaaagtggaa tataaagtct
    35221 ggcacttctt actgaccacg caagcgcgtt tcgtacagca tgatgagagc gatgaaagca
    35281 aactacactt atgcttcatt cgatacacat ttgtaaaagg ttgaatccat gaaaccaacg
    35341 acactactgc ttattttcac ttttttcgct atgccaggca tcgtatacgc cgagtcgcct
    35401 tttagctcac tgcagtccgc aaaagagaaa accacggtat tacaagattt acgtaaaatt
    35461 tgcacgccac aggcgtcatt atcagatgaa gcgtgggaaa agttaatgtt gtcagatgaa
    35521 aacaataaac aacatatacg cgaggccatc gtggcgatgg aacgtaataa tcagagcaat
    35581 tattgggaag cgttgggtaa ggtagaatgc ccggatatgt aagaacagca acaactactg
    35641 accaacgctt ctttacctga tatcaggatt tcttcatttt cacctttgca gtaaccaaca
    35701 atgcagtcag caacatcaat gtgccggaca acatcaacgg cgaagtcaga ccaatattat
    35761 ccagcgcata accgccgatt gccgcgccac aggtattagc aagctgaata accgccacct
    35821 gaatagaccc ggctttttct gcctgatcgg ccagcgagcg ggtgatccac gttgaccagc
    35881 cgacgggaac caatgcaaaa gttagccccc agataatcgc cacgccggta gcaacgattt
    35941 tatcgcttcc ccacaacgtc agtaccaacg cactcacagc cagtattaac ggcgcgcctg
    36001 ctaaggccag ttttaccgaa cgtttaagaa tgaatgacga aagcgacgta ccgataaagc
    36061 tggcaatgcc aaaactcaac agcaccagcg ttaagccatc cacgctgaat cccgccaggt
    36121 tcatatacac cgggcgaata tatgtgaaga aagcaaactg cccggcgaaa gacatgaaga
    36181 tggcgatcat ccctgccatc acacccggac gttgtaataa gcggaaagta ttttgtttct
    36241 gatgcgaggg ttcgcctggc agtgaaggca atgatttgat aatccagaaa atacacagca
    36301 cgcccatcac cgccgccgca ttaaagacat tgcgccaacc gataagctcg cctaaaaaac
    36361 tgcccaacgg cgcggcaatc accagcgcaa tagaaaccgc gccgaagatc accgacagcg
    36421 ccttcggcac cgtacgcggc ggcaccagac gcatggtcag cgacgccgac atcgcccaga
    36481 acccgcccag cgccagcccc agacaggcac gaccgattaa aagcaaactg aatgagttag
    36541 caaaggaaac cagcaagcag gagagcgtca gcaaaacggc aaacaaaata acaacgtagc
    36601 ggcggtcagt agcctgaatt gtctgggtaa taaacaaact ggcaaacatt gccacaaagg
    36661 cggtcacggt caccgattgc ccggcaaccc cttccgaaat gcctaaatcc tgggccattg
    36721 gcgtcaacaa actgacgggc aaaaactcaa cgataatcag acaggcgaca caaaacgcca
    36781 ccgagaaaac ggctgaccag ttcggtcggg tgatggcatc cgcgccgcgg ttttcggcaa
    36841 taaattcact catggtgtta cccgtggcat gtttcagggg aaaagccgac cactttaaca
    36901 ttgaaatgtg tgacgcattt aacgtttttg caacttttac ggtgttgctt gagctggagt
    36961 taagatcgaa acggaacagg gcaaagatta ccggatgcgg cgtgaacgtc ttatccaccc
    37021 gacaggtcag gcgcggtctg taggctgata agacgaagcg gttttaatgc agccagtgaa
    37081 cgccctcggc aggctgaaag aacgcgttat agcgcatctg aaaagcatta atatcctgca
    37141 tttcaggttc ggtttcgcgc agaaaaccca gcgccagcct gacctgagca tcggtaatcg
    37201 gcgcggcaag acgggctttt tgtaaaatcc gatgccattg cagcaggttc tgttcgctac
    37261 catcgacaat gacaatttgc cagatgagca acacctgcgc ggcgttttgc aggtgcgact
    37321 catccgggcg atgcaaataa cgtaaaaatt cgtcaccggc tgactttccc atttgatcga
    37381 ccatcgactc cacgggtacg acatcgatat tcagccgatc gctgagacgg cgaatcgctt
    37441 ttctggcccc ggatgtcagc acccgaaaac caaccacaaa cactaccgcc agcgtggcga
    37501 gcattatcca gatcatgagt tctcctgcgt aatcagaact catgatatct ggaaaccctc
    37561 gccagttact aatccaccag aataatcttc agtgcaaaca gagctgcgac caccaccaca
    37621 cacaggttca gatcgcgcca gcgcccggtg cacactttca tgatgcagta cgacataaag
    37681 ccgagtgcaa tcccttcggt gatcgagaaa gtaaagggca tcatcaccgt ggtaataaac
    37741 gcaggcaccg attcggtaaa atcatcccag ttaacgcgcg ccaggctcga agtcatcagc
    37801 acgccaacaa agattaacgc tccggcggtt gcgtaaggag gaaccatcgc caccagcggt
    37861 gagaagaaca taactaacag gaacataacg ccaaccacaa ccgcagtcag ccccgtgcga
    37921 ccaccgactg ccacaccaga agtactttca atataggcag taacagacga ggtgccgata
    37981 aacgcccccg ccaccgaact gacgctatca acatacagcg ccttattcat attggggaat
    38041 ttaccgttac tatctattaa gcccgcttta tcagttacac caattaatgt tcctgatgaa
    38101 tcaaataggt tgatcagcat aaaggagaaa atgataccgg cgagttcaag tgttaacgcg
    38161 ccgctcaaat ctacttcacc aatgacgccg ctaatatcag gcggaatgga atagacgccg
    38221 ctaaaatgaa catcaccgaa aaataatcca cagcaagacg tcaccacaat agaaaccagc
    38281 accgcggcat gaaaatgacg tgatgacaac acggttataa taaaaaaccc taaaatacct
    38341 aacaacacgc cgtgagaact taaatcgcca atcatcacca gcgtgtcttt attggcgaca
    38401 ataacgccag tattttttaa tcccattaag gcgataaata atccaattcc gctggtgata
    38461 ccaatacgta aacttaaggg aatgttggag atcatccagt accggatacg aaacagcgtg
    38521 agcaaaaata gtccaactgc gccccagaat attgcgccca tcccggtctg ccaggagatg
    38581 cccatcgccc ccacgaccac gaaggcaaag aaggcgttca gccccattgc cggagccagc
    38641 gccacgggta agttagcaaa tatccccatc gcaatactgc cgataccggc aatcaaacag
    38701 gtggtaacaa acaccacttt cgggtccatt tgtgccgcgc cgaggatttg cgggttcaca
    38761 aaaacgatgt acaccatggt taaaaaagtg gtcatccccg caatcaattc tgtgcggacg
    38821 gtggtcccat gctgaggtag tttaaataat cgcgaaagcg tccctgattc attactcacg
    38881 taatcggtat tgtcattatt catttttttg tccattggag gagatttaat cccttgcttt
    38941 tgtaaaacaa atgacaacaa cgattcagtg ataattatca catttcaatt gcacattaat
    39001 ggatattctt taataatctc gcgacgtttc tttatgataa ataataatca aattgataaa
    39061 atcaaaatga gaaaaatatg aataattcta ttaaccataa atttcatcac attagccggg
    39121 ctgaatacca ggaattgtta gccgtttccc gtggcgacgc tgttgccgat tatattattg
    39181 ataatgtctc tattctcgac ctgatcaatg gcggagaaat ttccggccca attgtgatta
    39241 aaggacgtta cattgccggt gttggcgcag aatacgctga tgctccggct ttgcagcgga
    39301 ttgatgctcg cggcgcaacg gcggtgccag ggtttattga tgctcacctg catattgaat
    39361 ccagcatgat gacgccggtc acttttgaaa ccgctaccct gccgcgcggc ctgacgaccg
    39421 ttatttgcga ccctcatgaa atcgtcaacg tgatgggaga agccgaattc gcctggtttg
    39481 cccgctgtgc cgaacaggca aggcaaaacc agtacttaca ggtcagctct tgcgtacccg
    39541 ccctggaagg ctgcgatgtt aacggtgcca gttttaccct tgaacagatg ctcgcctggc
    39601 gggaccatcc gcaggttacc ggccttgcag aaatgatgga ctaccctggc gtaattagcg
    39661 ggcagaatgc gctgctcgat aaactggatg catttcgcca cctgacgctg gacggtcact
    39721 gcccgggttt gggtggtaaa gaacttaacg cctatattac tgcgggtatt gaaaactgcc
    39781 acgaaagtta tcagctggaa gaaggacgcc ggaaattaca actcggcatg tcgttgatga
    39841 tccgcgaagg gtccgctgcc cgcaatctca acgcgctggc accgttgatc aacgaattta
    39901 acagcccgca atgcatgctc tgtaccgatg accgtaaccc gtgggagatc gcccatgaag
    39961 gacacatcga tgccttaatt cgccgcctga tcgaacaaca caatgtgccg ctgcatgtgg
    40021 catatcgcgt cgccagctgg tcgacggcgc gccactttgg tctgaatcac ctcggcttac
    40081 tggcacccgg caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc
    40141 agcaggtact ggtgaaaggc gagccgattg atgcgcaaac cttacaggcg gaagtgtcgg
    40201 cgagactggc acaatccgct ccgccatatg gcaacaccat tgcccgccag ccagtttccg
    40261 ccagcgactt tgccctgcaa tttacgctcg gaaaacgcta tcgggtcatt gacgtcatcc
    40321 ataacgaatt gattacgcac tcccactcca gcgtctacag cgaaaatggt tttgatcgcg
    40381 atgatgtgag ctttattgcc gtacttgagc gttacgggca acggctggct ccggcttgtg
    40441 gtttgcttgg cggctttgga ctgaatgaag gtgcgctggc tgcgacggtc agccatgaca
    40501 gccataatat tgtggtgatc ggtcgcagtg ccgaagagat ggcgctggcg gtcaatcagg
    40561 tgattcagga tggcggcggg ctgtgcgtgg tacgtaacgg ccaggtccaa agtcatctgc
    40621 cgttgcccat tgccggcctg atgagcaccg acacggcgca gttgctggcg gaacaaattg
    40681 acgccttgaa agccgccgcc cgtgaatgcg gtccgttacc cgatgagccg tttattcaga
    40741 tggcgtttct ttctctgcca gtgatccccg cgctaaaact aaccagtcag gggctatttg
    40801 atggcgagaa gtttgccttc actacgctgg aagtcacgga ataataaaaa agcccggcgt
    40861 catgccgggc aaaagtcacc agttacgttt atgccactgt caactgctga atttttttct
    40921 cgcggcggat tttgcgttct tccatcaccg ccactatcgc catcaggcag atacaaccaa
    40981 tcgcggcgat atcaagtgcg gcgaaggtgc ctgcccagcc ggtaaggccg aataccggcg
    41041 tcccatcggc aatcattccc agacctaact tggcaaagct gtcaccaatc aggtaagcaa
    41101 aggtaccttt aataccatcg gcagcgccaa tcgctttttt aggtacaaag ccaacagcag
    41161 ccacaccaat caacaattgc gggccaaaga ccaggaaacc caacgcaaag agagaagcca
    41221 gatagatata ttcgttgctg gcatgttgat acacaccgag cgtggcgata atcagcgcca
    41281 gcgcgatgca ggccaccagg ccacggcgac cgttcgccag gtcagagagc cagccccaca
    41341 gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt
    41401 tagagagttt cagttcctgg aacgcgtata cggttgacca ctggtcgata ccaatacgta
    41461 ccacatagag gaaaatgttg gcgaagcaca gcagccagat cactttgttt ttcagcacat
    41521 actcaacaaa gatctgccac ttggtcatat cggtagattc tgtctctttg tcctcttcgc
    41581 tgatctcctc gccgaacagt tcttcagctt tgccgaggcc ataagattcc ggggagtcgc
    41641 tgccgtaacg caggccgata aaaccgacaa tcagcgcgat aatcgacggg aagataaaca
    41701 tgccgatgac atggccatcg aacaggtaat ttgccccgaa cagcgccaca cctgctgcgc
    41761 ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag gaatgtcccg cgtttacgac
    41821 gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc gccggtactc tggaaaaagc
    41881 cgcttaaggc gtagaaggca atcatcagga acaggctaac cgagccgctg cccatactgg
    41941 cactgaagcc cagcatacaa atggcagaca ggatcagcat gaacggcagg aattgtttgg
    42001 tgtttttgcc gtcggcgtag taggaaacca gcgttttacc cacgccataa gtgatggaga
    42061 aacccaggcc gatcatcccc agctgcgtca tgctcaaccc gtaggtcgag atcatgtcgt
    42121 tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt caggtagccg ataaagacca
    42181 ccaggtagga ttgcatgaac ggtttgaacc acattttgcg ccgcacttcg agcggaaggt
    42241 ccagggtcgg cttgcgaacc tggtttaaga aagccagcat gggttactcc tgaaatgaat
    42301 acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag
    42361 gcaaaacctg gaaaatttct tagttttgcc ggatatgaag tgaaaaggtg agatgcatca
    42421 cgcttcgcgc ggtgcctggg cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc
    42481 ggcgatagag ataaccacaa aaaatccgct ccagtgccag gtatcgagta ctttcgc
//
LOCUS       DAHBHC010000040        41425 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHBHC010000040 DAHBHC010000000
VERSION     DAHBHC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.040"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            320..415
                     /locus_tag="M2A35_002564"
     CDS             320..415
                     /locus_tag="M2A35_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7198849.1"
                     /translation="MKNRKVVGEKVQDIADDLNVSISRLYHWLKM"
     gene            complement(761..2014)
                     /gene="amiC"
                     /locus_tag="M2A35_002565"
     CDS             complement(761..2014)
                     /gene="amiC"
                     /locus_tag="M2A35_002565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCB7198850.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2246..3577
                     /gene="argA"
                     /locus_tag="M2A35_002566"
     CDS             2246..3577
                     /gene="argA"
                     /locus_tag="M2A35_002566"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCB7198851.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3639..5465)
                     /gene="recD"
                     /locus_tag="M2A35_002567"
     CDS             complement(3639..5465)
                     /gene="recD"
                     /locus_tag="M2A35_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCB7198852.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEVSMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5465..9007)
                     /gene="recB"
                     /locus_tag="M2A35_002568"
     CDS             complement(5465..9007)
                     /gene="recB"
                     /locus_tag="M2A35_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCB7198853.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(9000..11888)
                     /gene="ptrA"
                     /locus_tag="M2A35_002569"
     CDS             complement(9000..11888)
                     /gene="ptrA"
                     /locus_tag="M2A35_002569"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCB7198854.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12064..15432)
                     /gene="recC"
                     /locus_tag="M2A35_002570"
     CDS             complement(12064..15432)
                     /gene="recC"
                     /locus_tag="M2A35_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCB7198855.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCHEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15445..15768)
                     /locus_tag="M2A35_002571"
     CDS             complement(15445..15768)
                     /locus_tag="M2A35_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCB7198856.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15753..16160)
                     /locus_tag="M2A35_002572"
     CDS             complement(15753..16160)
                     /locus_tag="M2A35_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCB7198857.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16157..16720)
                     /locus_tag="M2A35_002573"
     CDS             complement(16157..16720)
                     /locus_tag="M2A35_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCB7198858.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16711..17181)
                     /locus_tag="M2A35_002574"
     CDS             complement(16711..17181)
                     /locus_tag="M2A35_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCB7198859.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17366..18160)
                     /gene="thyA"
                     /locus_tag="M2A35_002575"
     CDS             complement(17366..18160)
                     /gene="thyA"
                     /locus_tag="M2A35_002575"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCB7198860.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18167..19042)
                     /gene="lgt"
                     /locus_tag="M2A35_002576"
     CDS             complement(18167..19042)
                     /gene="lgt"
                     /locus_tag="M2A35_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCB7198861.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19193..21439)
                     /gene="ptsP"
                     /locus_tag="M2A35_002577"
     CDS             complement(19193..21439)
                     /gene="ptsP"
                     /locus_tag="M2A35_002577"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCB7198862.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21452..21982)
                     /gene="rppH"
                     /locus_tag="M2A35_002578"
     CDS             complement(21452..21982)
                     /gene="rppH"
                     /locus_tag="M2A35_002578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCB7198863.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22667..23356
                     /gene="mutH"
                     /locus_tag="M2A35_002579"
     CDS             22667..23356
                     /gene="mutH"
                     /locus_tag="M2A35_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCB7198864.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDIIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23425..24138
                     /locus_tag="M2A35_002580"
     CDS             23425..24138
                     /locus_tag="M2A35_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCB7198865.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24276..24494
                     /gene="ygdR"
                     /locus_tag="M2A35_002581"
     CDS             24276..24494
                     /gene="ygdR"
                     /locus_tag="M2A35_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCB7198866.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24602..25642
                     /locus_tag="M2A35_002582"
     CDS             24602..25642
                     /locus_tag="M2A35_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCB7198867.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLDYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25674..26867)
                     /gene="lplT"
                     /locus_tag="M2A35_002583"
     CDS             complement(25674..26867)
                     /gene="lplT"
                     /locus_tag="M2A35_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCB7198868.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26860..29019)
                     /gene="aas"
                     /locus_tag="M2A35_002584"
     CDS             complement(26860..29019)
                     /gene="aas"
                     /locus_tag="M2A35_002584"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCB7198869.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29605..30636
                     /gene="galR"
                     /locus_tag="M2A35_002585"
     CDS             29605..30636
                     /gene="galR"
                     /locus_tag="M2A35_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCB7198870.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30643..31905)
                     /gene="lysA"
                     /locus_tag="M2A35_002586"
     CDS             complement(30643..31905)
                     /gene="lysA"
                     /locus_tag="M2A35_002586"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCB7198871.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGLVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32027..32962
                     /gene="lysR"
                     /locus_tag="M2A35_002587"
     CDS             32027..32962
                     /gene="lysR"
                     /locus_tag="M2A35_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCB7198872.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32949..33641)
                     /gene="ygeA"
                     /locus_tag="M2A35_002588"
     CDS             complement(32949..33641)
                     /gene="ygeA"
                     /locus_tag="M2A35_002588"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCB7198873.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33770..35188)
                     /gene="araE"
                     /locus_tag="M2A35_002589"
     CDS             complement(33770..35188)
                     /gene="araE"
                     /locus_tag="M2A35_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCB7198874.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWGVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKIAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35503..36264)
                     /gene="kduD"
                     /locus_tag="M2A35_002590"
     CDS             complement(35503..36264)
                     /gene="kduD"
                     /locus_tag="M2A35_002590"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCB7198875.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36294..37130)
                     /gene="kduI"
                     /locus_tag="M2A35_002591"
     CDS             complement(36294..37130)
                     /gene="kduI"
                     /locus_tag="M2A35_002591"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCB7198876.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37417..38598)
                     /locus_tag="M2A35_002592"
     CDS             complement(37417..38598)
                     /locus_tag="M2A35_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCB7198877.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38853..40082
                     /locus_tag="M2A35_002593"
     CDS             38853..40082
                     /locus_tag="M2A35_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCB7198878.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            40542..41174
                     /locus_tag="M2A35_002594"
     CDS             40542..41174
                     /locus_tag="M2A35_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7198879.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT        10393 a        11093 c        10010 g         9929 t
ORIGIN      
        1 tggaatacgc gctgacgaat atcgtgtaaa atggaacata ccgaagcaca tcgcactggc
       61 agggatttca tatctggaga aacaaagtga atatatgaag gcgagaatag caaagggaaa
      121 gctggatccc gcagaacaaa tagccatgat gagaataaag ggaaggcaac taaagtcaga
      181 tcctgactgg cgtaatcgct ccggttctat attgaagaaa caatatgccc gacgagatat
      241 ccttaaaaac agggtatggg aaagatctcc cagcataaaa agtgtctctg tggagttgaa
      301 aacagaagct ataaggcgaa tgaaaaacag gaaggtcgtc ggggaaaaag tccaggatat
      361 tgccgatgac ctgaacgtat ctatcagcag attgtatcac tggctaaaga tgtaggcgct
      421 catgtgctct caatgccttc aataaagccg ctatgcgcga accggcacat ctggtagact
      481 gttctcagtg agtcaagcaa actgcctact tttctaactt ttaatcatga ataaccagcg
      541 ccttgagtat agctggttcg aatatactcg ttgattcaca tcagataatg agtgtataat
      601 tcggtagata gcttgggttc gggaacagtg aatgccttaa gtttcaaaag gttagattaa
      661 cctcttgact gtggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
      721 ctgctctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
      781 tcgccccatc ggcaaaatac gctttaattc ccgcaagaat agactccgca acttcctgct
      841 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
      901 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
      961 ttttatgcag cttgttgatt ttaccgagct tattcagtac cgctttacca aacttcaggc
     1021 tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
     1081 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
     1141 ccgcagtact ggttgcacct ttggttgaga gcgcaaacac agaggaaccg ctcggctgac
     1201 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
     1261 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
     1321 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacgtctt
     1381 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
     1441 gcataatgac aatcggacga tcgcgccctg ctttacctgg ttgtggacca ctttgcgctg
     1501 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
     1561 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
     1621 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatcc
     1681 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
     1741 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
     1801 ccacgcgttc cgggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
     1861 ccgtcacacg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg
     1921 cagccaggct gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac
     1981 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
     2041 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
     2101 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
     2161 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata cactaatttc
     2221 gaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagt tggtcgaggg
     2281 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
     2341 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttatt
     2401 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
     2461 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
     2521 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
     2581 gtcgatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg tcagtggcaa
     2641 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
     2701 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
     2761 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
     2821 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
     2881 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
     2941 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
     3001 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
     3061 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
     3121 ggaaagcgct gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
     3181 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
     3241 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
     3301 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
     3361 ccgcagctca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
     3421 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
     3481 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
     3541 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaattg tcggatgcga
     3601 catgcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
     3661 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
     3721 tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
     3781 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
     3841 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
     3901 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
     3961 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
     4021 accggtcgac cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
     4081 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
     4141 cacaaaagtt ggtactcatt gaacgcctga atgattaaat ccggctcagc acgcgcttgc
     4201 agcagatcca gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct
     4261 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
     4321 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
     4381 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
     4441 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcccggc acgctcggcg
     4501 gtaaagccgg cattggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
     4561 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
     4621 agtctcgaca tcataggcag atcgatcatt gacacttcat ctaccaccag cacatcaaga
     4681 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc acccagcaat
     4741 cggtgcaaag tactggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
     4801 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
     4861 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagtaacttc
     4921 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
     4981 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
     5041 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
     5101 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
     5161 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
     5221 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
     5281 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
     5341 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
     5401 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
     5461 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg tcaatcaacc
     5521 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acaccacgca
     5581 ggaacagata aataacgccg ccaaagtggc gctcatagtc gtaatcagca atgcgatggc
     5641 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
     5701 gcatcgccgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
     5761 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
     5821 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcggatcaa
     5881 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcactaatc ggcaggtaaa
     5941 actccatctc cacctgttta tcgcggtcgg aaagctgatt caggctaaca cccgtttcat
     6001 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa cactggttcc cactgcgatt
     6061 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
     6121 cgaggtcttc aaacaaactg tgcaagaatg tccctggtga cgcaccgcgc gggaactgat
     6181 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
     6241 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caaaccggag tagctggtga
     6301 cacgccagtt atcgccgggc aatcgttgta gcgtcctcgc attcagttct gcagttaaag
     6361 catcattaac ctgccagggc tgtttatcac ccgtttgtgc cgtttgccag gcaatatcat
     6421 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
     6481 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
     6541 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgtgtc agcgccacgt
     6601 aaagcaaacg cagatcttcc gccagacgtt cggcctccgc gaggtcgacg ctttctggcg
     6661 cagcattaag atccagaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
     6721 ggacgcggaa attggtgata aacggcagcc agaccaatgg atattccagc cctttcgatt
     6781 tgtggatcgt gacaatctgc accagatgtt tatcactttc gagacgcatt tgttggctgg
     6841 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
     6901 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagtc
     6961 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
     7021 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
     7081 cgaccaccgc gtcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
     7141 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
     7201 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
     7261 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
     7321 cctggcggct gcgcaccagc acactgatgt ccgaagcacg caccggacgc gcgtcgtcac
     7381 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
     7441 cacatacctg cgccatggta ctttgataat cgccaacgcc gcagctttcg ccttccatca
     7501 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
     7561 tcccgactga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
     7621 ggctgaaaag cttattcacg ctgttcacca ttcctggtgc ggaacgccag ttggtgtcta
     7681 aagtgtagtg ggcgtgaact tcgctacgcg ccttcatata agtgaagata tccgcacccc
     7741 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
     7801 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
     7861 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcaccg ctttcgctac
     7921 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
     7981 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga
     8041 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
     8101 cccccccggc tttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
     8161 gcaactgata actgttggtc tcttcttctg cccaggcgct gatcttgtcg atccatttag
     8221 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
     8281 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtagc aatacgcgcc acaatttgcg
     8341 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
     8401 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
     8461 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
     8521 cctggtaacg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
     8581 caaaggcatt caggttgagc atgcgctggc aaaaaccgtg aatagtaaag actgccgctt
     8641 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
     8701 cttccagcag acgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
     8761 actcatggat attgctacgg atacgaccac gcaattctgc cgtggcagcc tcggtaaagg
     8821 tgaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc
     8881 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
     8941 cttcaatcag gcgctcaccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
     9001 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
     9061 actttccagc cttcagggtg tacatattcg gctttcccat tctggctgcc ggaaatctgc
     9121 gacaggatag ccatgccttg cggctcgact accgcctgat ggaagaaatc ggccagtttt
     9181 tgcggcgtca gcagtttaat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
     9241 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
     9301 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
     9361 aattttgcct ctgcagttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
     9421 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
     9481 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
     9541 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
     9601 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
     9661 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
     9721 tgtttttgca catcgcgtgc cagcgttgtt gcctgggctt cggtcatgtt gccaataacc
     9781 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
     9841 gtaatggagg gcaaaatttt gcgccgttca tctcgcgaga agtacggcac ttgcgagagc
     9901 atctgcgcgg gcataatcgc ctgctcgaac gctttgccct tttctgcgga atccatcatc
     9961 tggttatacc aggacttcgc ctgctcaagc tgatcttccg tggcggtata gctaaagtag
    10021 ccttccagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    10081 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    10141 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    10201 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcggcttt tggctcgctg
    10261 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    10321 ggatggtcat atttcttgtc tgacttaatc agcgagaaat cgtcaggaat ataagggtta
    10381 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    10441 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    10501 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    10561 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    10621 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgattgac
    10681 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcgatc
    10741 cctttttcac gtaacaaatt gaggtagcta aaaatagccg ccacaacctg atcgcgattc
    10801 gccagacctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    10861 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcggaa
    10921 agtgtacctg ggctgcgatt accaatcaga taggtaatca attcatcggt tttgctacgg
    10981 aatttcgccg agttgttatc aatacgaaac tctacgcgca acactttacg cggcagcgcc
    11041 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    11101 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    11161 tccggcagcg gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc
    11221 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    11281 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    11341 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    11401 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc agccaggcgg
    11461 tctaccgcac caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    11521 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    11581 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    11641 tacgcctcgg gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    11701 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    11761 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    11821 gcctgactta agggtgccca aagggcaact aacaacaata atgctttgaa ccaggtgctg
    11881 cggggcattc acggacctca taatcaactt aattttctgt ccagattcaa cacgttaacg
    11941 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    12001 tcagtccgtg actggtgcgc atcataaagc aagcggatag attgcgcaat ttttatacag
    12061 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    12121 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    12181 ttcgccacgc accatcatgt tgccttcgta agcctgcagg aatttcgtac gggctttttg
    12241 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    12301 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc
    12361 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    12421 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    12481 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    12541 caagccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    12601 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    12661 catctcatgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    12721 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    12781 taactgctga ttgatttgat aacgactaag cccttcaaga ataaatggct cggtgtcggg
    12841 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    12901 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    12961 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    13021 acgagcatag ctttgtcgtt cgcctggctg gtagttttgt ggatcaaacg gcatccgggt
    13081 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    13141 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    13201 cgggaaacgt tcactgttat cctgaatgga gcgcccgata tagctgatat aaagtttttg
    13261 ttgcgcggaa attaacgctt ccaggaacag atagcggtca tcatcgcgac ggctacggtc
    13321 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    13381 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    13441 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    13501 acgctgcgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    13561 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    13621 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    13681 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    13741 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    13801 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    13861 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    13921 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    13981 gtcaaaccgc gccgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa
    14041 gcggctgtct ggcaacgata acaggctgat aaacgcttcc agtaccggat gcgactgccg
    14101 cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    14161 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    14221 agtaagtgtc gggtcttctt ccagcatcgc cagcaggcga tcgtgcaaaa cttcaacttc
    14281 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    14341 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcac ggttttccag
    14401 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtgacat cgacaaaggc
    14461 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    14521 accccatgaa gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    14581 cccggcattt tcgctatcac gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    14641 ctgacgggtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    14701 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct gtagcgcctg
    14761 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtagccccgg
    14821 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg
    14881 ccagcgcggt tgcccgagtt gatgggtata ttccaccaac gccttccaca acggcgcttg
    14941 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    15001 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgaag aaagctggaa
    15061 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    15121 ctccaacaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    15181 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    15241 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    15301 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    15361 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    15421 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    15481 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    15541 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    15601 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    15661 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    15721 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct
    15781 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcaatgact
    15841 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    15901 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    15961 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    16021 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    16081 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    16141 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    16201 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    16261 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    16321 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    16381 tcagacgaaa tccaatctgg tcagactctt ttaccggttc gcgatcccag ataccgttac
    16441 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    16501 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    16561 gcagccagat ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta
    16621 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    16681 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    16741 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    16801 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    16861 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    16921 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    16981 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    17041 ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata
    17101 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    17161 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    17221 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    17281 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaaccgacg cacactggcg
    17341 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    17401 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    17461 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    17521 ccatatgatt gctgtacagg tgcgtgtcgc ctcccgtcca gacaaaatcg cccacttcca
    17581 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    17641 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    17701 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    17761 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    17821 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    17881 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    17941 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg
    18001 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    18061 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    18121 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    18181 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    18241 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg
    18301 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag
    18361 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    18421 caccttccag cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    18481 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    18541 ctgggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    18601 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    18661 aaacctggaa gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc
    18721 caatcaggcc gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat
    18781 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga
    18841 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg
    18901 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    18961 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    19021 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    19081 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    19141 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    19201 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa
    19261 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat
    19321 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    19381 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    19441 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    19501 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    19561 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    19621 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    19681 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    19741 catcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    19801 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    19861 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    19921 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    19981 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    20041 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    20101 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    20161 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    20221 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    20281 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    20341 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    20401 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    20461 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    20521 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    20581 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    20641 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    20701 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc accggcggca aagcgtttgc
    20761 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    20821 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    20881 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    20941 gatattgccc aaacagggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    21001 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    21061 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    21121 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    21181 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    21241 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    21301 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    21361 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    21421 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    21481 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    21541 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    21601 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    21661 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    21721 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    21781 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    21841 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    21901 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    21961 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    22021 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    22081 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    22141 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    22201 gagcattctg aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt
    22261 acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac
    22321 gggttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    22381 actgaaccac taaaaactgg agtttcatcg cacgtcaagg ctgtaaatgg aaacagtagt
    22441 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    22501 tttcagtgac cagatttgga aaacctattg cagtgttgcg caactcgatt accggcagcc
    22561 taaaaagcaa tataaatcag ggaattgaac aacacatgtg gaaaaagtta cactgcaaat
    22621 attcggcaca taattgctgt ttgtttttta atcaaggtat catgacatgt cccaacctcg
    22681 cccactgctc tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc
    22741 tggttataca ttgggagaac tggcggcact tgccgggctt gttacgccag agaatttaaa
    22801 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    22861 taaacctgag caagattttg ccgctctggg cgtggaactt aaaactatcc ctgtggatag
    22921 tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg
    22981 ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa cgcgtactgt ggataccggt
    23041 tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag
    23101 cccgaatgaa gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatcat
    23161 tgttctcggt caggttgagc ggatcaccgc tcgtcacggg gaatatttac agataagacc
    23221 gaaagcagcg aatgcgaaag cgcttaccga agccattggt gcccggggcg aacggattct
    23281 gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca
    23341 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    23401 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    23461 cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    23521 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    23581 ttggcgggag ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc
    23641 ctgacaaatc cgctttttac aatattcagt caggaaattt ctgcccgtga tttgattctg
    23701 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa
    23761 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt
    23821 atgctgctgg atattatctt tagcctcgac tcggtgatta cggctgtggg tctgtcagat
    23881 cacctgttta ttatgatggc agccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    23941 cgctcgattg gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc
    24001 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    24061 tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaactt gattcgcaac
    24121 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactccttt
    24181 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt tataaacaga
    24241 ctattatcat aggtaggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    24301 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    24361 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    24421 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    24481 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    24541 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    24601 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    24661 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    24721 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    24781 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    24841 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
    24901 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    24961 cctacagact gattacctcg atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
    25021 cttcggcaaa ctcgattata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
    25081 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    25141 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
    25201 tattgtcacc attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc
    25261 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
    25321 gctgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
    25381 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
    25441 cgccagacgt catggcctgg accctgctca gatggcgctc gcgtttgtac gccgtcaacc
    25501 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
    25561 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tgcatcaggt
    25621 ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga
    25681 cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc gaagccaatg
    25741 ccaatgggca cgaccgggat gcctatcatt accgccagcg agtaaatgcc cagcatcaac
    25801 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    25861 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccatcacg
    25921 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga aaaaatcagt
    25981 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaact
    26041 aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    26101 gtgggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    26161 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    26221 ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    26281 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    26341 ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga catgccagtc agccagcaca
    26401 ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat taaaccgtta
    26461 gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    26521 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    26581 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    26641 aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc
    26701 atttgcagga tgggctggct ccactctgga tagaactgcg ctttcagtaa cgccagagtg
    26761 gcaaacagta gggcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    26821 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    26881 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    26941 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    27001 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    27061 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    27121 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    27181 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    27241 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    27301 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    27361 gccctcttcg ataccaggga ccgacaacag gcgcgcatcc attcctggta gaatacgccc
    27421 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    27481 cacgccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    27541 ttcttgtaat ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    27601 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtgccg aacaacacgg tgcaactgcg
    27661 gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    27721 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    27781 taacgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    27841 attcgccaga atgcttttat ggctatggac gacgcctttc ggatggcctt cagaaccgga
    27901 ggtaaagagg atcagcgcct cttcttccgg ctgctgttta acctgtgcca gacgcggcat
    27961 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    28021 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    28081 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    28141 cccttttacc ccggcagtgt agttcatcat tgcgggcatg cggcgacggg cgatggcccc
    28201 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    28261 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    28321 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    28381 tgcactcagt aaagattcgt acagcgtttc acgcgggcgc accgccattc gcgcttccat
    28441 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    28501 cggcatcgcc acctgcgttg gtggcaaaat atgcagagta atttgcggga acaagcgacg
    28561 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    28621 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    28681 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    28741 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    28801 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    28861 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    28921 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    28981 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    29041 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    29101 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    29161 gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct gcacgacgac
    29221 tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt
    29281 cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc gcaggctggt
    29341 gtaattcatg tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact
    29401 ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta
    29461 accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac
    29521 gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga aagaatgtaa
    29581 gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc
    29641 gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ctaaagccag tgaagcttcc
    29701 cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt
    29761 gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg
    29821 tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta
    29881 ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga gcaactgatc
    29941 cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc
    30001 tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa
    30061 aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt
    30121 cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa
    30181 gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattgcggc caatgaccgg
    30241 ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg
    30301 ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggtgcg
    30361 atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt
    30421 gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc
    30481 gtgacgatgg cgacccaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc
    30541 ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc gtcattcagt gtcaactccg
    30601 tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc
    30661 cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag
    30721 aacttctggt aacagcggac ggctattgta gttggatgac attgatgcgc catatgcccc
    30781 tgtatcatgc agtaccagat aatcacccgc cttcacttcc ggcaaggcgc gggtttcaac
    30841 atttccccct tcctgctggg taaagacatc gcccgattca cataatggcc cggcgacgac
    30901 ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg cactgatatg
    30961 gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccagcat caaccagcac
    31021 aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaata cgccagactg
    31081 cgctaccagg aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgggc
    31141 gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc
    31201 accctgttga taaggaacag aaagcccacc gcccgcagag atagcctgta aatcctgacc
    31261 gaattcgatg acctgacgca ccatagcacc acacacctgt tccagatggg cataatcaac
    31321 gccagaacca atgtgcatgt gaatgccgac cagctgcaga tgatgacgtt gtatcacgtc
    31381 cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt
    31441 ggttttttgg ctatgtccgt gaccaaaccc cggattaacg cgcagccata cccgatgccc
    31501 tggcgaaacc aggcccagtt ggtcgagcat atcaacagaa cccgcattca ccggaatttg
    31561 caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc
    31621 atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga
    31681 gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa tattggaaca
    31741 ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat
    31801 aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt
    31861 ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagata
    31921 agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga
    31981 gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa
    32041 cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc
    32101 acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt
    32161 gatcgggcta aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact
    32221 gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc
    32281 agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc
    32341 gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt
    32401 aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga
    32461 tttaggactc actgagacac tccatacgcc tgcgggaaca gaacgtaccg aattactctc
    32521 tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac
    32581 gccggatgat tttcagggtg agaactacat cagcctttcc cgtactgaca gctatcgcca
    32641 gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca
    32701 cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg tggttaaccc
    32761 gctcaccgca ctggattatg cggcaagcgg tttagtggtg cggcggttca gtattgcggt
    32821 tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggtgcaggc
    32881 gtttagtgag catttacaag cggggttacc gaaactggtc acttctcttg acgctatttt
    32941 gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcagtatca
    33001 aacacaggca aaacactgcg ctcttctggc accagtaaac caatttctgt gcagccaaaa
    33061 atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt
    33121 gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt aattttcgcc
    33181 cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccgtcag ccgcccgcga
    33241 taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc
    33301 ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac
    33361 tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc
    33421 gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg
    33481 cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag
    33541 tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg
    33601 gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg
    33661 ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccgatc
    33721 tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga
    33781 tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt
    33841 tggtttccgg aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga
    33901 gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggtc gcgccgataa
    33961 tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca
    34021 gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg
    34081 caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt
    34141 tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac
    34201 caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg
    34261 cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa
    34321 agcccgctat tttgaagata cgcggcgcat agtacatgat gatgttcata ccggtaaact
    34381 gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct
    34441 taaacagtgc ccaaccgcct tgttttaact tcaggctttc acgaatttcg ttgagttctt
    34501 ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac
    34561 gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca
    34621 ggactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg
    34681 ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga
    34741 tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag
    34801 acgcgatccc gacagcaatg cccagcacca cacgagcggc gattaacatc tctacgctgg
    34861 tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt
    34921 atttacgacc caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga
    34981 gcatcatgct actaaccccc cattcctgca aacgactggt cagcacaaag tgatcggtaa
    35041 tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg
    35101 cagcagctac cgaaacaaac atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta
    35161 aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg
    35221 aaaaaatacg tgaacaactc acgcaggtgt caggtcggaa acagcataaa tatggattaa
    35281 attgctgcga catgtcgtta tgtgatggat attccaattt tcaaattaag ttgaattatt
    35341 gagattatta ttaaccacct aattttacag cagataaaat tcataaagtt cattaattga
    35401 taattaatat ggattatttc ataaccatga tatggattat gatgatctac aggtataaaa
    35461 aaacctgcca tgcggcaggg tcataaaagt aagaagaatg aattaacgcg ccagccaacc
    35521 gccatccacg gcaatggtat aaccattcac ataatctgaa gcgctggagg caaggaacac
    35581 tatcggcccc atcaggtcac tcggcagtcc ccaacgacca gctggaatgc ggtcgagaat
    35641 ttccgcgcta cgttgttcat ctgcccgtag ttgttgagta ttgttggtcg ccatgtaacc
    35701 cggagctatc gcattaacat taatgttgtg tttagcccat tcgttcgcca tcaatcgcgt
    35761 cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa
    35821 ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga taaagtgttt
    35881 cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca
    35941 gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt tattcaccag
    36001 gatatcaata tgaccaaatt cagctaccgc gcgatccagc agtccaggaa tgccatcaat
    36061 ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat
    36121 ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc
    36181 caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa ctttaccttc
    36241 gagagaaaat gcacttaaaa tcataacaat accttattcg tttatgccca caactagcgc
    36301 aaatctttaa cggccacatg gtccatatca tcaaagacct ggttttcacc gaccatgccc
    36361 cagataaagg tataagcttt ggttccgacg ccggaatgaa tcgaccagct cggggagatc
    36421 accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggctgccc catcatgtgg
    36481 aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg gcgctcgtga
    36541 gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag ccccatactc
    36601 aattggcagg tttccagcac atccgggaca aaatatttgt taattgtgcg acggttactg
    36661 gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt tttggtggga
    36721 tacgtcgtat gcgcgggtgc gcaattgtaa taaaactttg ccggagtggc ggtatcaata
    36781 ctggcaaaga caacttcttt tgcaccttta cccacataca gggcgtcgcg gtgaccgatt
    36841 tcatagcatt ggccatcgac agtaatcgta ccggcaccgc caatattgat aacacctaac
    36901 tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc gccaacggaa
    36961 accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg gctgtaaacc
    37021 atggtgtact catcggcgac aaatacctct tcaaccaaaa attcattgcg cagcccttgg
    37081 gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc ttacgtccac atcaacctcc
    37141 gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg ttttctctat
    37201 aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa tgtttcattt
    37261 ttaagttaag gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa
    37321 atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa cagattaatg
    37381 acggcagata tttatcggta aggaggaaat gcagagctac tcgtcacgtt caatggtcaa
    37441 tgccacgccc tggcccccgc cgatacaaag cgttgccagc cctttgcggg cattacgttt
    37501 caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc
    37561 gagtgcgatc gcgccaccat tgacattgac ccgacgctca tcccactcaa gcatcttgcc
    37621 aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga catcagccaa
    37681 ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa
    37741 tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa
    37801 cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga
    37861 tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc
    37921 ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt
    37981 actttggatc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg
    38041 cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc
    38101 gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc
    38161 atcatgcacc agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc
    38221 agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc
    38281 ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagcccag aaccgcaaac
    38341 gtcattaata gtgattgcag aaacgctatt aggaagacca cctttaatag ccgattgcct
    38401 tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc
    38461 atatgcagga acgccggtac gttctattaa cgctttcacg accagactac caagttccac
    38521 ggcggaatga cccgctaacg caccgcgaaa gcagccgata ggtgtccgta acgccccgac
    38581 aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt
    38641 taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct
    38701 gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc caccaataga
    38761 ggggtagata gggcgttaat ctcccatact taacctggtt tatggtaaat tgccctccat
    38821 tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa
    38881 ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgctc ttccttccta
    38941 ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt
    39001 taacatattg gccacataaa gccttatgcc agttcatcct ctcatcgaaa acatcagcag
    39061 gtgaagggat aacgggcgca gtaacacact actatggcaa gaagattggt aatctgatta
    39121 ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca
    39181 actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc cgtatgttgg
    39241 tgagtctggg tgttgtatta atcctgaatc tcatttttct gatgggacgc catgcaacca
    39301 ttcgggtaat gggttttttg gtattcccat tgattgccta tttcttattt ctttccattt
    39361 accttgtcgg tagttggcaa cctgatctat taacgaccca ggtagagttc aatcagaata
    39421 cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc catacgccca
    39481 ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct atggataaat
    39541 gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg ttctttgtct
    39601 ttagctgcct gctttctatt ccaccttcgt atattgaagc ggctaaagaa gaaggggtca
    39661 ccattttatc ggcgctttct atgctgccga acgccccagc atggctgtca atttccggga
    39721 ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt ggcgttattg
    39781 aaggtgccac ggaggtcgtc aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg
    39841 catttaaccg cgcactatca attatgttgg tatcgctgat caccttcatt gtttgttgca
    39901 ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt gccatgatac
    39961 ttttcatcat gcctacgttg tcaacgtatc tgatcccggc gcttaaaccc tggcgttcca
    40021 tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg ttctttagct
    40081 gatatccatt gtgaatggtt caaaattgct acaattttga accattccag ttttcataat
    40141 gttcattcgt tattattccg cactatcact tccctcatca tatcctagta ttatcagtta
    40201 gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg cataacttag
    40261 attcgcaaga tatcttccgg tcattcaaaa acaatttgcg atatagatat tctcatacca
    40321 tgagatagtt atctgaacca ccctcaacca ggtaagatta aatcagtgaa acataatcat
    40381 atttaataac gagaaatgca ttttaaaatg tcttaatcag aattatagca aatacagatt
    40441 aatcgcaatt tttcccagca aaattacaaa atcatggtgt attgacatca tcaatcacca
    40501 atgaaattat gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt
    40561 ataaaagtct tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac
    40621 cgcgtttgca gtactgcgca aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt
    40681 ttaatcgtgg tgtggtagaa ggtatcttaa caaaaaacca caatgcgaga ttaagcggat
    40741 atatttttgt cgatttttca gtaagttttt tacgtctatt ccttgaaaaa gactggattg
    40801 actatcttgc aagtactgac atggggattg ttttagtcag cgaccgcaat atgcaatcgt
    40861 tagctaatta ctggcgaaaa cacaactcag caatatctgc tgtcatatat aatgatgatg
    40921 gtcttgatgt tgccaatgaa aagatcagac aactatttat tggtcgttat ttatcattta
    40981 ccagaggtaa cacattaact cagatggaat tcaccataat ggggtatatg gtttctggtt
    41041 ataatccata tcagattgct gaagttctgg atatggatat ccgtagcatc tacgcgtaca
    41101 agcaacgaat cgaaaagaga atgggtggta aaataaacga attatttatt cgttcacatt
    41161 cggtccaaca ttgatactca acgaccagcc agaatcatac tctgtttata cgggcattta
    41221 ttgaaatgtc cgtattttat aaataggccg tcccctcaag agtaaacacc atagtattta
    41281 aaaccatcac acgaaaaatt cagaagcatt acgaaatatg aatttacaaa caatagtggc
    41341 ataaatgtta accatgttaa tttacgtaaa gttttacgtt gcaacattaa agcctcattt
    41401 caatcatcat gataaatata aaatt
//
LOCUS       DAHBHC010000041        40986 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHBHC010000041 DAHBHC010000000
VERSION     DAHBHC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.041"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="M2A35_002595"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="M2A35_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCB7198880.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="M2A35_002596"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="M2A35_002596"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCB7198881.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGYGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="M2A35_002597"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="M2A35_002597"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCB7198882.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="M2A35_002598"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="M2A35_002598"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCB7198883.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="M2A35_002599"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="M2A35_002599"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCB7198884.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="M2A35_002600"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="M2A35_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCB7198885.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /locus_tag="M2A35_002601"
     CDS             complement(8992..9609)
                     /locus_tag="M2A35_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCB7198886.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="M2A35_002602"
     CDS             complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="M2A35_002602"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCB7198887.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="M2A35_002603"
     CDS             complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="M2A35_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCB7198888.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSERLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="M2A35_002604"
     CDS             complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="M2A35_002604"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCB7198889.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            14482..15561
                     /locus_tag="M2A35_002605"
     CDS             14482..15561
                     /locus_tag="M2A35_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCB7198890.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15576..15896
                     /locus_tag="M2A35_002606"
     CDS             15576..15896
                     /locus_tag="M2A35_002606"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCB7198891.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            15947..18244
                     /locus_tag="M2A35_002607"
     CDS             15947..18244
                     /locus_tag="M2A35_002607"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCB7198892.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            18210..19088
                     /locus_tag="M2A35_002608"
     CDS             18210..19088
                     /locus_tag="M2A35_002608"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCB7198893.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            19090..19431
                     /locus_tag="M2A35_002609"
     CDS             19090..19431
                     /locus_tag="M2A35_002609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCB7198894.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19418..20269)
                     /locus_tag="M2A35_002610"
     CDS             complement(19418..20269)
                     /locus_tag="M2A35_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7198895.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(20418..22151)
                     /locus_tag="M2A35_002611"
     CDS             complement(20418..22151)
                     /locus_tag="M2A35_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCB7198896.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(22334..24985)
                     /gene="ppc"
                     /locus_tag="M2A35_002612"
     CDS             complement(22334..24985)
                     /gene="ppc"
                     /locus_tag="M2A35_002612"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCB7198897.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(25337..26488)
                     /gene="argE"
                     /locus_tag="M2A35_002613"
     CDS             complement(25337..26488)
                     /gene="argE"
                     /locus_tag="M2A35_002613"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCB7198898.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            26642..27646
                     /gene="argC"
                     /locus_tag="M2A35_002614"
     CDS             26642..27646
                     /gene="argC"
                     /locus_tag="M2A35_002614"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCB7198899.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            27654..28430
                     /gene="argB"
                     /locus_tag="M2A35_002615"
     CDS             27654..28430
                     /gene="argB"
                     /locus_tag="M2A35_002615"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCB7198900.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            28491..29864
                     /gene="argH"
                     /locus_tag="M2A35_002616"
     CDS             28491..29864
                     /gene="argH"
                     /locus_tag="M2A35_002616"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCB7198901.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            30363..31577
                     /locus_tag="M2A35_002617"
     CDS             30363..31577
                     /locus_tag="M2A35_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCB7198902.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            31644..32927
                     /locus_tag="M2A35_002618"
     CDS             31644..32927
                     /locus_tag="M2A35_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7198903.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            33179..34096
                     /gene="oxyR"
                     /locus_tag="M2A35_002619"
     CDS             33179..34096
                     /gene="oxyR"
                     /locus_tag="M2A35_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCB7198904.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(34079..35479)
                     /gene="sthA"
                     /locus_tag="M2A35_002620"
     CDS             complement(34079..35479)
                     /gene="sthA"
                     /locus_tag="M2A35_002620"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCB7198905.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35810..36460
                     /gene="fabR"
                     /locus_tag="M2A35_002621"
     CDS             35810..36460
                     /gene="fabR"
                     /locus_tag="M2A35_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCB7198906.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36460..36819
                     /locus_tag="M2A35_002622"
     CDS             36460..36819
                     /locus_tag="M2A35_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCB7198907.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36859..37959)
                     /gene="trmA"
                     /locus_tag="M2A35_002623"
     CDS             complement(36859..37959)
                     /gene="trmA"
                     /locus_tag="M2A35_002623"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCB7198908.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      38073..38263
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38328..40172
                     /gene="btuB"
                     /locus_tag="M2A35_002624"
     CDS             38328..40172
                     /gene="btuB"
                     /locus_tag="M2A35_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCB7198909.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            40117..40974
                     /gene="murI"
                     /locus_tag="M2A35_002625"
     CDS             40117..40974
                     /gene="murI"
                     /locus_tag="M2A35_002625"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCB7198910.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9279 a        10236 c        11321 g        10150 t
ORIGIN      
        1 gttccactcc gcgccgctct tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca cagttaatgt gggcactggc cgtaaacccg
      421 catagtttac cgtacaggcg ctaccgtgac atcgggtaat ccacctgctg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaccacaat gtttactctg gtgcctgaca tttcaccaat
      601 aaagcccagg gaacatcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccc atccatcttt ccagtaccta
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgtg gcaacccaac
      781 gcgcgatgtg gttcagcgag cactggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aacgaccgtc tttttgaaac ctggcgatct
      901 gctggtagcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 gctggcagat aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttaggtgccg atctggtgtt
     1201 gcattcatgc acgaaatatc tgaacggtca ctcagacgtg gtggccggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgcatgga
     1381 gttggcccag cgtaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgccagca
     1501 aaaaggctat ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgggt ggactgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatctcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtggggcacg gtgaagtgtg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gccagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtca tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgctac cgtcggagcg
     3601 ggcttgccga tcaaccatac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagtggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcaagggt taaccgagcc tgacccgcgt
     3781 gatgaccttt ccggcaaaga cgtaatgcgc aagctggtga ttctggcgcg tgaagcaggt
     3841 tacaacatcg aaccggatca ggtacgcgtg gagtcgctgg tgcctgccca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaac
     4021 ggcaaagcgc gtgtgggcgt ggaggcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccgtgcgata acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc
     4141 cgtgggcctg gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtt
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatta agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aacggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ctgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagccg gaaatgtatg cttctgacct ggtgacgctg
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaggt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgctcaac ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt ctttgatgtc gaaagctacc tgcgttttcg tgaccgctgc
     5161 gtatcggcgg gtattgatgt ggaaattatt ccgggaattt tgccggtctc gaactttaaa
     5221 caagcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcacaaatg
     5281 tttgacgggc tggacgacga tgccgaaacc cgcaaactgg tgggagcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acacagtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgggaa aataaggtta tcagccttgt tttctccctc attacttgaa
     5581 ggatatgaac ctaaaaccct tttttataaa gcatttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatattcata acaccacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaaccca ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggcctgccga
     6061 ctggggcagt tacgccggtc tgtttattcg tatggcctgg cacggcgcag gtacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcaggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaacgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggctgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 tctgttcaag tatgagtggg tacagacccg cagtccggct ggagcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac
     6901 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cacagcaact gacgctgacc gcgccggaaa tgactgcgct
     7621 ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg actgacgaat cgaaagagct gttcgaaggt cgtgaccgtg aaaccggcga
     7801 agtgaaatac acagccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtagcggaa gtttacgcca gcagcgatgc ccacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgtct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     8041 actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcaa tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgcct
     8581 atacacccgt catccgcgcg tggatttatt atcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccga tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt ggagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtgggagtt
     9721 tcagattact ggcacagagt gccggatgcg gagtgaacgc cttatccggc ctacaaaagc
     9781 acgcatattc aatatattgc agagatttcg taggcttgat aaacgtagcg catcaggcaa
     9841 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     9901 ggaaacgctg accgtactgg tcggctacca gcagagcggc gtaaacctga tctggcgtcg
     9961 cgccgccagg catgttgtga atggtttcac cttctgcaca tgccgcttct gccacaattc
    10021 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
    10081 ccgcatggct aagtgcagct acggtttcga tttcctcaac cggtgcgttt tccagaacca
    10141 gctgcgtcag cgtaccaaat gccacttttt caccgtgata atagtgatgc gcgtccggga
    10201 tagcggtcag gccgttatgc accgcgtgag ccgccgccag cccgccgctt tcaaaaccaa
    10261 cgccgctcaa ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct
    10321 gttcggcggc aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag
    10381 ccagtgccag cgccgcctgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag
    10441 agcaagcacg cgcttcaaac caggttgcca gcgcatcgcc gatacccgct gctaacagac
    10501 gtgcaggtgc gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca
    10561 acagcagata gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg
    10621 gtgcatcggt agaggcgata gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca
    10681 gtgctttggc agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg
    10741 cagtctccgc gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg
    10801 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa
    10861 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
    10921 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc
    10981 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtgttcg
    11041 aacttctcta tagctgactc taccgcaggg gtgttgagca tttgttgcgc tacatctaag
    11101 ggcagggtga tggattcaca tcctgccagt aaacagtcca gcgcctgacg cggcgtttta
    11161 aagctggccg ccagcaccat gctttctggt gcgtgcattt ctaacagcgc ttgcagctcc
    11221 tgaaccgtac gaatgccgtc cccgccctgg gcatctacgc ggttaacata cggagcaacg
    11281 tattttgccc ctgccagcgc ggccagtaac ccttgtgcag cactgtacac tgcggtgcca
    11341 agcgtagtaa taccctcttt tttcagcatc ttaatggcgg ccagacgttc ggaagtcacc
    11401 gggattttca ccacaatgcc cggaatggcg tcgcgcaggc gcttcgcttc ttccaccata
    11461 ccctgcgcgt cacgactcat agtctgagca aacagaatgc cctcatcacc aatcgctttt
    11521 tgcagacgcg gcagaacttc ccatatggac tccttgctgg cagcgataat gctcgggtta
    11581 gttgtcaccc cggcaatggg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    11641 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    11701 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    11761 tgcggcgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca
    11821 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac
    11881 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac
    11941 taaagcccag accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac
    12001 gcggatagcg gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    12061 ctttgctgcg ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct
    12121 gttgggtcat gcggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    12181 ttgcctggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    12241 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg
    12301 gcagcacgga acctttcgct cccagctcac cgcacagacc aatccatttg ccctggcgat
    12361 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt
    12421 gacgagtaac cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac
    12481 taccaatact aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg
    12541 gtacttccag cataatgcca agctggatct tctcatcaaa cggaatgtgt tcgttacgca
    12601 gttgctgttt ggcttccgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    12661 tcatcgggat catgattttc aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt
    12721 gtgtggtgaa cagcgaggcg tattcttcat aaatacgcac agcgcgataa ccgaggaacg
    12781 ggttagcttc tgccggaatg ttcagataat caacgggttt gtcgccgcca atgtccatag
    12841 tgcgcacaat aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca
    12901 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac
    12961 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag
    13021 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    13081 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt
    13141 ctcctggctc aaccacaatc gccccagcgt tgccgtcgat ataaatcgtt tgatgctgcc
    13201 acggagtaag ggcatcaata tctacaccaa ccagtgtcgg aatgttgaac gaacgggcaa
    13261 gaattacggt atgtgaggtg gtgccgccac ttttcagtaa cagacctttg aggtgatttt
    13321 tatccagttc gaggaactgg ctgggagtca gttcatcagc catacaaatg gcgggctgcg
    13381 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    13441 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    13501 cttcgcaaaa gtgattcgca ctggtgacaa tcgcttcggc gcagcttaat ccggcgctga
    13561 cgcctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgcgcttcca
    13621 gaatcgcgct ggtggcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca
    13681 gcgtcaggcc gttttccagt gcggattgct cggcgtcaac gcctttggct gcgggaagat
    13741 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc
    13801 tgcacacggt gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    13861 gttccagttc gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc
    13921 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    13981 ggcagttatc gcccgccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    14041 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    14101 tttcaacgtg gcttgccgga cgtgcatgta cgccgttagg tagctcacaa ataaattcca
    14161 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    14221 gcaacggcct gccatgtgac aaatctgcca aaagctggac aaatgtgatg taaccgtcaa
    14281 tttgcgacgc gtctcacaag acgctgtttt gcggcatgct tccggtttat cgcaagttat
    14341 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    14401 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    14461 tcgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    14521 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    14581 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat
    14641 ctgaaaaaac tgtttgatat cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgca
    14701 gcgtacatcg gttattccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    14761 tgggttggta acagctttgg tgcgggcttc tttggtgcac tgatcgccgg gattatcggc
    14821 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    14881 cctatcttca ttattcctat cgtcggcaca ctgattaccg caggcatcat gatgtggggc
    14941 ttgggcgagc ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag
    15001 cagggcagca ttgttatgct ggcggtgatc atgggtctga tgctggcgtt cgatatgggc
    15061 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    15121 accgtggtgg ccatcgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg
    15181 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg
    15241 gtgatgggct gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    15301 cgtgtcattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    15361 ggtgcgcagt gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag
    15421 ctgggttata tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    15481 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    15541 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    15601 catgtccttc aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga
    15661 aagcgaaagg ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac
    15721 tgactgcgga agatgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca
    15781 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa
    15841 aacgtgctga cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc
    15901 ttctcccgct cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    15961 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct
    16021 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    16081 agcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    16141 cggtaaccgc accgtaaaac cgcgcgctgg gattatgtcg ccggaaatgg acccttactg
    16201 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagtga
    16261 agaagataaa cgtatctacc gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    16321 agatttcatc aacgggcaga tgacagatga agtaaaagcc gcgaccagca cgcagatttt
    16381 cagcatcaac cagacagata aaggccaggg gcacattatt attgattacc cacgcctgct
    16441 gaatcacggg ctgggggagc tggtggcgca gatgcagcaa cattgtcagc aacagccgga
    16501 gaatcacttt tatcaggccg cgctgttact gctggaagcc tcgcagaaac acattctgcg
    16561 ttacgctgaa ctggcggaaa caatggcggc aaactgcaca gatgcccagc gtcgcgaaga
    16621 actgctgact attgcggaga tctcccgcca taacgccgaa cataagccgc agacgttctg
    16681 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    16741 ttcgctatcg ttggggcgct tcgaccagta tatgttgccg ttctatcagg catctttaac
    16801 ccagggcgaa gatccggcgt tcctgaaaga actactcgaa tctttatggg tgaaatgcaa
    16861 cgacatcgtg ctgttgcgct ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg
    16921 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    16981 gttcctttgc cttgacgctt atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    17041 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg
    17101 caccggtatt ccgcaaatct ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg
    17161 cgtttcgctg gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    17221 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    17281 ctgcctgcat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    17341 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat
    17401 cggccatcgc gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga
    17461 aaaaggtcgc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    17521 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    17581 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg
    17641 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    17701 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    17761 gaacccgcgc ggcggttact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    17821 gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg
    17881 cggcttgtca cctatgctgg gccaggacgc acaagggcca acagcggtac tgaagtcagt
    17941 cagtaagctc gataacacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    18001 ggcgaccctg gaaggtgaag cgggattacg caaactggcc gacttcttac gggcgtttac
    18061 ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    18121 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    18181 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    18241 gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt
    18301 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    18361 gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg
    18421 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggtgcgt
    18481 ttgaacggat tggtcgcgat atcagccttg acgctctgga acgggaagtg atgaaagatg
    18541 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    18601 aggccgagtt tgctacccgt tttttacagc gactgcggct gtggggcgtg tcatgcgcca
    18661 tcgaaactgc cggagacgcg ccagccagca agctgttacc gctggcgaaa ttgtgcgatg
    18721 aagtgttgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    18781 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgaaggtgtc aacgtgatcc
    18841 cgcgtttgcc gctgatcccc ggtttcacac tcagccggga gaatatgcag caggcgctgg
    18901 acgtgctgat cccgctgaat atcaggcaga tccatctgtt accgtttcat cagtacggcg
    18961 aaccgaaata ccgtctgctg gggaaaacat ggtcgatgaa agaggtttct gcgccgtcgt
    19021 cagccgatgt ggcaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    19081 gaggttaaaa tggcatacct ggtggcagta accgcctgcg tcagtggcgt ggcgcatact
    19141 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    19201 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgccgggcg
    19261 gatgttgcgc tgttgattac ggatatcgag cttgctggtg ccgagcgatt tgaacattgt
    19321 cgctacgtgc aatgcagtat ctacgcgttc ctgcgtgagc cgcagcgggt gatgagcgcg
    19381 gtacgtaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    19441 ctgtcagttg gctgtggtac tgccgacgat attccgacgg cgagcgttcg gtgtttttac
    19501 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    19561 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    19621 attcgttaaa accaatggcc cccgtctttt gaaacaggtg cgagagatag tttggtgaaa
    19681 tatagaacgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    19741 aatcacgaat agcttcgaat agagcctggc tgcgtgaggc ggtctgtatt tggctgccga
    19801 gcaaatcgcg gcagtggcta agcaggctgg cgacgataag ccttgccgtt tgctgctcct
    19861 gtggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaaccaatg cgcgggccgc
    19921 gtcgggcgac gtgttgcttc gccagatttt gatactgttt gccatcccat tgcacgacgc
    19981 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt agtagcgggg gcttgccatt
    20041 gtggaaaatt ccagccacca gcgggaacgt acagcacatc gccgggaact aacgttgctc
    20101 cagcgccggt atcaacaaac tcaccttcca gcactatttc cagccgtgga aaatcgacct
    20161 gatacgcaag atcgggcgcc ggaccgttac tgctggcaaa gtaaatctgg cgcagggaca
    20221 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    20281 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    20341 gcctgagggg agttcgatgt aaacattgcg gcagtatcgc atccggcaaa tagcgccaga
    20401 cgcgatacaa aggtcattta ctgattaccc acctgatcgc catacggcag cgtgtcgtaa
    20461 tcgatcagcg cgtttttctt gtacgggtta ccaatccagc gggtagtttc tttgaactgc
    20521 ggattcacca ctgaacgggt tggatcgtaa ccgtcgtaag ataagcctgc taaatctgac
    20581 caggtgtgga tcagttccgc caggctgtat ttacgatcaa catcctgcga gaaatcacgc
    20641 ggatgagtcg cttgccattt ttccgacgtc cacagcagga acggaatggt gtacatgtgg
    20701 cgcgtcgggt tgtcttcgtt acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct
    20761 tcaccgtggt cagagaaata aaccaggaaa ccgttcgggt ttgctgcttt aaagtcttta
    20821 atcaggctgg caaccacatg atcgttatac aggttagcgt tgtcataatc gttatatgac
    20881 tccagctctt ccgcgtttaa tccaggcgga acatgatcgg tattgccatc aaacttgccc
    20941 tgattttccg ggtagcggta tttgtatttg atatgcgtac ccagcagatg aacgatgatc
    21001 agtttcttcg gcgccgggtc cttcagcact tcctggaatg gtttcagcac gttggtgtcg
    21061 tattcacgcg cactctgcgt acgttgctgg ttcatataga actgtttgtc ggtctgcttc
    21121 gaaaataccg tcagcatggt attgcgggcg gtcatcgtct gctggttggt gatccagaag
    21181 gttttataac ctgcctgttt catcatgttc atcagcgacg gctgcgtcag atacagatcc
    21241 gggttctttt cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt gtacggacga
    21301 gacgtaacta cgttattaaa cacggtcaga ttcggatcgg ttttatgcag cgcatccagc
    21361 tccggcgtgg tttcacgcgg ataaccgtac agactcatgc gtccgcgctg ggtcgactcg
    21421 ccaatcacca gcactaaagt gcgcggttcg ttacccgatt catctttgaa attagccagt
    21481 ggcggcaagg cattattttc attcagtaac tttgttagcg agtttagttg ctgacgatac
    21541 tgataatagc cggtcaggaa ttgccacggt gcggcaggct ccatacgcga ggccaggtta
    21601 tccaacgttt tctcaaacgg cttgtttttg ataaacgtat tcatggcgat cggatgcaga
    21661 atcaagccat aaagcagggc aaaagagaca acataacgcc acggctttgg aatatagacc
    21721 gggcgcaggc gtgtccacag cagaactgcc accgccgtat aggccagcgc gataagcaca
    21781 attttcaggc tgaaatactg gcttaaatac tcgctggctt cgttggtgtt ggtttcgaac
    21841 atcacaaaca gaacgctctg cgagaactcc tgcccgtaga tgacgtagta gcatagcgcc
    21901 gccagagagg ccgcccatag caccacgccg attactgcgg caataatttt aatccgcttc
    21961 ggaaagagga ataccgggat caaccacagc gagctgaata acagcgagtc gcgaatgccg
    22021 ttggtgccac tataaccact gatgtaaata atggcctgta gcagagtaga gaaaaaccaa
    22081 aagtagagca gtgcccaacc cagggctttc cagctaaaaa ggggtttagc ctggacttct
    22141 gtggaatgca tagtaagaac ctgtcttgaa aaaaatatcg ccgaatgtaa cgacaattcc
    22201 ttaaggatat ctgaaggtat attcagaatt tgaataaaat gcagacagaa atatattgaa
    22261 aacgagggtg ttagaacaga agtatttcag aaaaccctcg cgcaaaagca cgagggtttg
    22321 cagaagagga agattagccg gtattacgca tacctgccgc aatcccggca atagtgacca
    22381 ttaacgcttg ttcgacgcga ggatccggtt cctggccttc tttttctgcc tggcgggagc
    22441 ggtgcagtaa ctcggcctgc aatacgttca gcgggtcggt gtaaatattc cgtagctgaa
    22501 tagactctgc aatccacggc agatcggcca ttagatggga atcgttggca atcgccagca
    22561 ccactttgat gtcttcttct tgcaggttgc gtaactcttt acctaacggc cacagtgctt
    22621 tgtctaccag gcgttggtca tagtattccg ccagccacag gtctgctttg gcgaagacca
    22681 tctccagcat gccgagacgc gtcgagaaga atggccaatc gcggcacatg gcttccagct
    22741 cgctctgttt gccatcttcg accacttttt gcagcgccgt acctgcaccc agccaggcgg
    22801 ggagcatcag gcggttttgc gtccaggcaa aaatccacgg tatggcgcgc agcgactcga
    22861 cgccgccggt tgggcgacgt ttcgccggac gtgaacccaa cggcagtttg cccaattctt
    22921 gttccggcgt agcggagcgg aagtaaggca caaaatcttt gttttcacgt acgtagccac
    22981 ggtagagatc gcaggagatg actgacagtt catccataat gcgacgccag ctctctttcg
    23041 gctcaggtgg tggcagcagg ttggcttcca gaatcgcccc ggtgtacagc gacaggctgc
    23101 tgacggtgat ttctggcaga ccatatttaa agcggatcat ctcgccctgt tcagtcacgc
    23161 gcaggccacc tttcaggctt cctggcggtt gtgacagcag cgccgcatga gcaggtgcgc
    23221 cgccgcgacc aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata cccgcttttt
    23281 cgcaggtttt gattaatgca tcctgtgcct gatattgcgc ccaggaagct gccatcactc
    23341 ccgcatcttt tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc
    23401 cacgatacca gtcaatattg agcagctggg tcatgacatc gttggcgttg ttcagatcat
    23461 cgagggtttc aaacagcgga gcaaccggca tcgcaaaccc gatacccgct tctttcagca
    23521 gcaggtggac agccagtacg tcggacggcg ttttcgccat cgagatcacg taggcggcaa
    23581 tggagccttg cggcgcttcg gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg
    23641 cgcttggttg ccagttgcgc ggcagaagcg gacgtttgga gttcagttcg cggatcagga
    23701 acgcctgttt gtcggcctct gaccagcttt cgtagtcgcc gataccgagg tagcgggtca
    23761 gctcgcccag cgcttcggta tggcgggtgc tctcctgacg gatatcaata cggaccagcg
    23821 gtacgccgaa acatttcacg cggcgcaggg tgtcgagcag atcgccgttg gcgataatac
    23881 ccatgccaca cgcctgaagt gactggtagc aagcgtagag cggttcccac agttcttcgt
    23941 tttgtgtcag cagcccttct ggttttggca gttcttcgcc tttcaggcgc gcttccagcc
    24001 atgcctgtgt cgccatcagg cgagaacgca ggtttttcat cagatagcga tacggttctg
    24061 cggcaccttc ttcgccaacc agcgccagca gttcaggggt cgcttcaacc atcgacagtt
    24121 cagaaaccag cacctgaata tctttcagga acaaatcggt ggctttccag cggctgagta
    24181 gcaggacgtg gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg tcgccgccca
    24241 tccacgaagt aaaacggacc ggaacaaatt cgacgggcag tttgtagccg aggttctctt
    24301 ccagttgttc gttcagttcg cgcaggtaat ttggtacgcc ttgccacagg ctgttttcca
    24361 ctacggcaaa gccccatttg gcttcatcta ccgggcttgg acgcagctta cggatttcat
    24421 cggtatgcca tgactgggcg attaactggc gcaggcgacg catcagctgg ttgtgttcgt
    24481 agtcagcgat atctttgtta tcgagctgtt ttaagcaggc gttcacttcc accattttgt
    24541 ggatcagtgt acgacgggta atttcggtag ggtgagccgt gaggaccagt tccagcgaca
    24601 gcgattccac tgcttttttg atggtgtctt cgctcagttc cggctggttt ttcagtttac
    24661 gcagggtgcg ggcgatcact tccgggttgc tggcagcttc gcctttcggc gaaatgctgt
    24721 ggtattgctc ggcggtgttg gccaggttca ggaactgact aaacgcacgc gcaacgggca
    24781 gcagctcgtc gttcgacaaa ttttgtaagg tggtgagtaa ctcctggcgg ttagcatcat
    24841 tgccagcgcg tgaagatttg gacaacttac ggatagtttc tacgcgttca agaatgtgtt
    24901 ctcccaacgc atccttgatg gtttctccca gcactttgcc gagcatactg acattactac
    24961 gcaatgcgga atattgttcg ttcatattac cccagacacc ccatcttatc gtttgatagc
    25021 cctgtatcct tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt
    25081 tataaagcca cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata
    25141 aatagcagga atttacgtca ttaaattcac gacgctttaa ataagcgtaa cttatggaaa
    25201 tgttaaaaaa tcgccccaag taacaccaaa ggtgtaggtc ggataagatg cgcaagtatc
    25261 gcatccgaca ttattgcggc actggagttt gacaacagtg ccggatgcgg cgcgagcgcc
    25321 ttatccggcc tacgttttaa tgccagcaaa aatggtgaat tacctgggtt atcagttcgc
    25381 gggtgggctt gataaaccgt gtttccagat attcatcagg ttgatgagcc tgattaattg
    25441 agccaggccc caacaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt
    25501 agttcaccac ttcggtcttt gctccgagca atttctcaac tacttcaacc agttgatgat
    25561 tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg
    25621 gccagcgttc gctcaccgga gccaatgcat cgttgagcaa accattaagt tcattgagtg
    25681 tcatgccagg cagcggacga atatccatat gcaactcaca gcaagcgcaa atacggttag
    25741 aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg gtatggcacg gtaaacgctt
    25801 cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt
    25861 gcattagttc gatagcgtta actccgcgtg ctggatcgct ggagtgcccc gactggccct
    25921 gaatacggat ggcgttagag atatgacctt tatgtgcgcg taccggttgt agtgacgtcg
    25981 gttcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc
    26041 cggccatact ggtttcttca tcagcagtcg ccagaatgta gagcggtttt ttcagtttcg
    26101 tgacgtcgac atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg
    26161 tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatca cgcgtccagc
    26221 gaccgtcatc aaatggtacc gtatcggtat gccccgccag caacaagccg ccagccccct
    26281 gtccgatact tgccagcata ttaaatttgt tgcgagttcc tggcacaggc tgcacttcca
    26341 cattgaagcc taaatcttta aaccagtccg ccagcagagt gattaaatct gcattgcttt
    26401 gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct
    26461 cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa
    26521 tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag
    26581 ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc
    26641 gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta
    26701 tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga
    26761 tgcgggcaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg atctgccgtt
    26821 gcagccgatg tcggatatca gcgagtttag cccaggggtg gacgtagtgt ttctcgccac
    26881 cgcccatgaa gttagccacg atttagcgcc gcagtttctt gaagcgggct gcgtggtgtt
    26941 cgacctttcc ggcgcgtttc gtgttaacga cgccaccttc tatgaaaaat attacggctt
    27001 tacccatcaa tacccggaac tgttggaaca ggcagcctac ggtctggcgg agtggtgcgg
    27061 taataaatta aaagaagcga atttgattgc ggtgccgggc tgttatccga cggcggcaca
    27121 gctggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat
    27181 caacgccacc agcggcgtga gcggtgcagg gcgtaaagcg gccatttcaa acagcttttg
    27241 tgaagttagc ctgcaaccgt atggcgtctt tactcatcgc catcaaccag agatcgccac
    27301 acacctcggt gctgacgtta tcttcacccc acatctgggc aatttcccgc gcggcattct
    27361 cgaaaccatt acctgccgcc tgaaatcggg tgtgacccag gcgcaagtcg cgcaagtgtt
    27421 acagcaggcg tatgcccata aaccgctggt gcggctgtat gacaaaggcg ttccggcgct
    27481 gaaaaatgtc gttgggctgc cattttgcga tatcgggttt gccgttcagg gcgagcatct
    27541 gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtacagtg
    27601 cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga
    27661 atccattaat tatcaaactg ggcggcgtac tgctggatag tgaagaggcg ctggaacgtc
    27721 tgtttagcgc actggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtgcacg
    27781 gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa
    27841 acggcctgcg ggtgacgcct gctgatcaga tagacattat caccggagca ctggcgggaa
    27901 cggcaaataa aaccctgttg gcatgggcga agaaacatca gattgcggcc gtaggtttgt
    27961 ttctcggtga cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggtcatgttg
    28021 gactggcgca gccaggttcg cctaagctta tcaactcctt gctggagaac ggttatctgc
    28081 cggtggtcag ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgacc
    28141 aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca
    28201 gcggcattct cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg aaagcagaac
    28261 aactgattga gcagggcatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg
    28321 atgcggcccg cacgctgggc cgtccggtag atatcgcctc ctggcgtcat gcggagcagc
    28381 ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg
    28441 ccggaggcgc agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt
    28501 ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc
    28561 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc
    28621 tggtcacggt aggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg
    28681 tgttgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata
    28741 tccatagctg ggtggaaggc aaactgatcg acaaagtggg ccagttaggc aaaaagctgc
    28801 ataccgggcg tagccgtaat gatcaggtag cgactgacct gaaactgtgg tgcaaagata
    28861 ccgttagcga gttactgacg gctaaccggc agctgcaatc ggcgctggtg gaaaccgcac
    28921 aaaacaatca ggacgcggta atgccaggtt acactcacct gcaacgcgcc cagccggtga
    28981 cgttcgcgca ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa agccgtttgc
    29041 aggatgcgct taagcgtctg gatgtcagcc cgctaggctg tggcgcgctg gcgggaacgg
    29101 cctatgaaat cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta
    29161 acagtctcga cagcgtttct gaccgtgacc atgtgttgga actgctttct gctgccgcta
    29221 tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag
    29281 cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg ccgcagaaga
    29341 aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggggcgttaa
    29401 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag
    29461 acaaagaagg tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc
    29521 tggtgctgga cggcattcag gtgaaacgtc cacgttgcca ggaagcggct cagcagggtt
    29581 acgccaacgc caccgaactg gcggattatc tggtggcgaa aggcgtaccg ttccgcgagg
    29641 cgcaccatat tgttggtgaa gcggtggtgg aagccattcg tcagggcaaa ccgctggaag
    29701 atctgccgct cgacgagttg cagaaattca gcccggtgat tgacgaagat gtctatccga
    29761 ttctgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc tcaccgcagc
    29821 aggtggcgca ggcaattgct tttgcgcagg cgcggttaga gtaatttgtt tatattgctc
    29881 ccctgaaaat ggggagctta ccacgcctac aaagtgcgac acttcgtagg cggataaggc
    29941 gtttacgcca catcaggcag aaatttgtca gcattctggt gagcttaaat ttaacgccca
    30001 accagaacca ttatttgtca cattcctttt tatcattgtg tcttctttac cttcacactc
    30061 cccatgaaaa tcatattttc aaatacatca accaatacat attttaatta attgaatata
    30121 attattttct attgctgtgc ccatttttaa tgattgatgg agtttgttat gtgattattt
    30181 aacgtgttgt tttaacgata atggttaatt attcaggtgg tttttgaggg tattttttct
    30241 cctcggtgga aatttttgtt tttaatgtga tcacggttga ataatctctt tttctctctt
    30301 gtatggtagt aggcgtaaaa ttaagtttga aacgtctatt tacaacacag gagattgcaa
    30361 taatgaaaat tattgctgca gatgtttttg ttacttgccc agggcgtaat ttcgttacgc
    30421 ttaaaataac cactgaaagc ggactttgtg gattaggaga tgccacgtta aatggtcgcg
    30481 aattgtcggt cgcttcttat ctgaaagatc atctttgtcc acaactaatt ggccgcgatg
    30541 ccagccgtat cgaagatatc tggcaattct tttataaagg tgcttactgg cgacgtggcc
    30601 ccgtcacgat gtcagccatt tctgccattg atatggcgtt gtgggatatc aaagctaaag
    30661 cggcaaatat gccattgtat caactgcttg gtggtgcttc tcgcgaaggc gtgatggttt
    30721 attgccacac caccgggcgg acgattgatg aagtccttga agactatgca aagcaccagc
    30781 agatgggctt taaggcgatt cgtgtgcagt gcggtgtacc gggtatgcaa actacttatg
    30841 gcctggcgaa gggcaaaggc ctggcgtatg aaccggcaac caaaggcctg tggccagaag
    30901 agcagttgtg gtcttcagaa aagtatctcg attttacgcc gaagttattt gaagcggtgc
    30961 gtaataagtt cggttttaac gaacatctgt tgcatgacat gcaccatcgc ctgaccccca
    31021 tcgaagcggc gcgctttggg aagagcattg aggattaccg cctgttctgg atggaagatc
    31081 cgacgcctgc agagaaccag gaatgttttc gtctgattcg ccagcatacc gtgacgccga
    31141 ttgcggtcgg tgaagtgttt aacagtatct gggactgcaa gcagttaatc gaagagcagc
    31201 tgattgacta tattcgcacg acgatcaccc atgcaggcgg cattaccggg atgcggcgga
    31261 ttgccgattt tgcatcgctt tatcaggtac gtaccggttc gcatggccct tcagatttat
    31321 cgcctgtttg tcatgcggcg gcgctgcatt ttgacctatg ggtaccaaat tttggcgttc
    31381 aggagtacat gggctattcc gaacaaatgc ttgaagtttt cccgcacagc tggcgcttcg
    31441 aggaaggtta tatgcaccca ggcgatgaac cggggctggg tatttctttc gatgagaaac
    31501 tggcggcgaa atatccgtat gacccggcgt atctccccgt cgcacgactg gaagatggca
    31561 cattgtggaa ctggtaataa cggttcgctt aattattttc tgaatagtca tcaaatcgat
    31621 taattccgct caaggagcta aaaatggacg ttgatgtgtc aaccagtgtt gcgggtaata
    31681 aaccgcaacg gattcgtcgt attcagacag tgaccctggt tttattattt atggcgggga
    31741 tcgttaattt tctcgaccgt tcgtcattga gcgtggcagg tgaagcgatt cgtggcgagt
    31801 taggattatc ggcgacagag tttggtgttt tgctttctgc attttctctg tcttatggtt
    31861 tttcacaact accttccggt attttgctag accgctttgg cccacgaatt gttttaggcg
    31921 caggcttaat attctggtca ttaatgcagg cattaacagg aatggttaat tcttttagcc
    31981 actttatttt aatgcgtatc ggtctgggta ttggcgaagc gccatttatg cctgcgggag
    32041 taaagtcgat caccgactgg tatgcacaaa aagaacgcgg cacggcgctg gggattttta
    32101 actcatctac cgttatcggt caggctatcg cgcctcctgc tcttgtattg atgcagctgg
    32161 catggggctg gcggacgatg ttcgttatca tcggcgtggc agggattctg gttgggatct
    32221 gttggtacgc gtggtatcgc aaccgggcgc agtttgtcct gactgacgaa gaacggacgt
    32281 atctctccgc gccggttaaa ccgcgtccac agctgcaatt tagcgagtgg ctggcgctgt
    32341 ttaagcatcg gacaacctgg gggatgattt taggtttctc tggcgtcaat tataccggtt
    32401 ggctctacat cgcgtggtta cccggttatt tgcaagccga gcaaggtttc agtctggcga
    32461 aaaccggttg ggtggcggcg attcctttcc tcgcggcggc agtcgggatg tgggttaacg
    32521 gtattgttgt cgatcgactg gcgaaaaaag gctacgacct ggcgaagacg cgtaaaacgg
    32581 ctattgtctg cggtttgatg atgtcggcat taggcacgtt gctggtcgtg caatcttcct
    32641 cgccagccca ggcggtagcg tttatctcaa tggcgctatt ctgcgtgcat ttcgctggaa
    32701 cgtctgcatg ggggctggtg caggtgatgg tgtcagaaac aaaagtggct tccatcgccg
    32761 ggattcaaaa ctttggcagt tttgtttttg cttcctttgc tccgatcgta accggttggg
    32821 tagtggatac cacacactcg tttaatctgg cgttggttat cgcggcctgt gtgacgttca
    32881 ccggggcgct gtgttacttc tttattgtca aagatcgcat tgagtaatcg ttactatcag
    32941 gctctcttgc tgtgggcctg tagaataaaa aaaagcggat cctggagatc cgcaaaagtt
    33001 cacgttggct ttagttattc gagttgagaa actctcgaaa cgggcagtga cttcaagggt
    33061 taaaagaggt gccgctccgt ttctgtgagc aattatcagt cagaatgctt gatagggata
    33121 atcgttcatt gctattctac ctatcgccat gaactatcgt ggcgatggag gatggataat
    33181 gaatattcgt gatcttgagt acctggtggc attggccgag caccgccatt ttcggcgtgc
    33241 ggcagattcc tgccacgtta gccagccgac gcttagcggg caaattcgta agctggaaga
    33301 tgagttgggc gtgatgttgc tggagcggac cagccgtaaa gtgttgttca cccaggcggg
    33361 aatgctgctg gtagatcagg cgcgtaccgt actgcgtgag gtgaaagtcc ttaaagagat
    33421 ggcaagccag cagggcgaga cgatgtccgg gccgctgcac attggtttga ttcccacagt
    33481 tggaccgtac ctgctaccgc atattatccc tatgctgcac cagacctttc caaagctgga
    33541 aatgtatctg catgaagcac agacccacca gttactggcg caactggaca gcggcaaact
    33601 cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag tgccgttgtt
    33661 tgatgagcca atgttgctgg ctatctatga agatcacccg tgggcgaacc gcgaatgcgt
    33721 accgatggcc gatctggcag gggaaaaact gctgatgctg gaagatggtc actgtttgcg
    33781 cgatcaggca atgggtttct gttttgaagc cggggcggat gaagatacac acttccgcgc
    33841 gaccagcctg gagacgctgc gcaacatggt ggcggcaggt agcgggatca ctttactgcc
    33901 agcgctggct gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc cgtgcattaa
    33961 gccggaacca cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc tgcgcagccg
    34021 ctatgagcag ctggcagagg ccatccgagc aagaatggat ggccatttcg ataaagtgtt
    34081 aaaacaggcg gtttaaaccg tttaacgcag ctacccgata ggcttccgcc atggtcgggt
    34141 agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc gccacctttc tgttccataa
    34201 tcgcctgacc gatatgaata atttcggcag cgcgctcgcc aaagcagtga atacccagaa
    34261 tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc cacgttcatg ccgacgattt
    34321 gtgcgcgtgc cagatgttta aactgggcgc ggcccacttc atatggcact ttcatcgcgg
    34381 tcagctgctg ttcggttttg cccacagagc tgatttccgg gatggtgtaa atgccggtag
    34441 ggatatcttc aatcagatgc gcggtggctt cgccttttac cagcgcctgc gcggcaatgc
    34501 gcccctggtc ataggccgct gatgccaggc tcggataacc aatcacgtcg cccaccgcgt
    34561 aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt cagctgtccg cgactgtcag
    34621 tttccagccc aatgttctgt aacgccagcg aatcggtatt accggtacga ccgttggcat
    34681 agagcaggca gtcagctttc agttttttac ccgacttcag atgcatgatc acaccgtcgt
    34741 cacagccttc gatcttctcg tactcttcgt tgtgacgaat cactacgcca ctgttccaga
    34801 agtgatagga gagagaatct gacatctctt gatcgagaaa tgccagcagg cgatcgcggg
    34861 tgttgatcag atccactttt acatccatac cgcggaagat cgacgcatat tcacagccga
    34921 tcactccagc accatagata agtacatggc gcggttcgtg gtgcatgcta agaattgagt
    34981 cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt tggatgatat ggacgagagc
    35041 cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac gctgccgtcc gggcaatcca
    35101 gcgccaacgt atgctcgtca acaaagcgag cgtttccctg caatatttca cagtgattac
    35161 gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat cacgttatcg gcatggttaa
    35221 ggatatcggc aaaagaagag cggagcagtc gggaatggtc gctgtaaagt gggttttgat
    35281 tgaattctat aatgcggctg acggcgtgac ggagagcttt cgacgggatg gtgccccagt
    35341 gggtgcaacc gccgccaaca ttttgataac gctcgataac tgcgacgcgc gcaccttgct
    35401 taaccaggcc cattgcagcg ccttcgccgc cggggccgga acctattact atggcatcgt
    35461 aatcgtagga atgtggcatg gtagggctta cctgttctta tacataaaag caacagaatg
    35521 gtaacatttt atcgcgggta agccaattga tccccgtcat ttatctggct atatcctgag
    35581 cggcctttgc tttgtctgtt tcttactttt gccctgacgt tttattggat ttttatcgac
    35641 gatactctcc gtttaagcgg caggtttccg ctgtacgtaa aagaaccggc caaagaattg
    35701 cagtaaatat gttttattgc gttaccgttc attcacaata ctggagcaat ccagtatgtt
    35761 cattctctgg tatagtgcca gcagtacttt tggcaaggat tcagacatcg tgatgggcgt
    35821 aagagcgcaa caaaaagaaa aaacccgccg ttcgctggtg gaagccgcat ttagccaatt
    35881 aagtgctgaa cgcagcttcg ccagcctgag tttgcgtgaa gtggcgcgtg aagcgggcat
    35941 tgctcccacc tctttttatc ggcatttccg cgacgtagac gaactgggtc tgaccatggt
    36001 tgatgagagc ggtttaatgc tacgccaact catgcgccag gcgcgtcagc gtatcgccaa
    36061 aggcgggagt gtgatccgca cctcggtctc cacatttatg gagttcatcg gtaataatcc
    36121 taacgccttc cggttattat tgcgggaacg ctccggcacc tccgctgcgt ttcgtgccgc
    36181 cgttgcgcgt gaaattcagc acttcattgc ggaacttgcg gactatctgg aactcgaaaa
    36241 ccatatgccg cgtgcgttta ctgaagcgca agccgaagca atggtgacaa ttgtcttcag
    36301 tgcgggtgcc gaggcgttgg acgtcggcgt cgaacaacgt cggcaattag aagagcgact
    36361 ggtactgcaa ctgcgaatga tttcgaaagg ggcttattac tggtatcgcc gtgaacaaga
    36421 gaaaaccgca attattccgg gaaatgtgaa ggacgagtaa tgaaacaagc aaatcaagat
    36481 agaggtacgc tgctgctggc gttggttgct ggcttatcga ttaatggtac tttcgcagcg
    36541 ctgtttagct ccattgtgcc attttctgta ttcccgatta tttccctggt gctgacggtt
    36601 tactgcctgc atcaacgtta tcttaatcgc accatgccgg taggcttgcc gggtctggca
    36661 gctgcctgtt ttattctcgg cgtactgctg tacagcacgg tagttcgtgc ggaatatccg
    36721 gatatcggct ctaacttctt cccggcagta ctctccgtca ttatggtgtt ctggattggc
    36781 gcgaagatgc gtaaccgtaa gcaggaagtt gctgagtaat cggtattatg ccggatgctg
    36841 gcgcatccgg catgggtttt acttcgcggt cagtaatacg ccgcactcca tatggtgcgt
    36901 gtaggggaac tgatcaaaca gagccagacg ttcgaccttg tgcgtctggc ttaatgtttc
    36961 cagattcttg cataacgttt ccggattgca ggagatatac agaatacgtg gatacgcctg
    37021 caccattttc tcggtttcac tgtccagacc gctgcgcggt gggtcgacaa aaatggtttc
    37081 gcactgataa ctcttcaagt ctatcccttg caggcgatta aactcgcgta caccattcat
    37141 cgcctgagta aattcttctg ccgccatacg aataatttgt acgttatcaa tatggttagc
    37201 tgcgatgttg tattgcgcag cagcaaccga cggcttagcg atttcggtgg ctaatacccg
    37261 atcaaaattg cgcgccagtg ctaatgaaaa gttaccgttg ccgcagtaca gctccagtaa
    37321 atcgcctttt gagcctttgg ttacgtccag cgcccattcc agcatctgaa tattcatcgc
    37381 cgcgttcggc tgggtaaaac tgttttctac ctgacggtag atcatctctt tccctgcgac
    37441 cggcagacgt tcatcgatgt aatcttgatc cagctcgatt ttggttttcg ttgcccgacc
    37501 aatcagatgc acattcagat tctgcgcgcg cagtgcatcg cgcagggctt ccgcttcctg
    37561 acgccactca tcatccagct ttttatggta caacagggaa accaccgcct gattactcag
    37621 cgtggtgagg taatcaatct ggaacaactt gtggcgcaga acgggattat tacgcacacc
    37681 cgcaatcatc gccgtcatca actggttgat gagttcactg gcagcgggga agctatccac
    37741 gcggatgcgg cttttggttt gttgatcgaa aatgatgtga tacaggtcat cgccatcgtg
    37801 ccagatgcgg aactccgcac gcatccggta atgactgacc ggcgagcgaa acacttccgg
    37861 aaccaggtca gaaaacggtg ccatcatact ttgcaaacgt accacttttt cggctaactg
    37921 cgcttcatac tgttctgttg gaaggtgttc gggggtcatg atgtatcctg aaaaattaaa
    37981 gtacgcggcg attgtaggga ttgctcatca gatgtccaga tcttgatgaa ttcctatttg
    38041 tgagctacgt ctggacagta acttgttaca acctgtagca tccacttgcc ggtcctgtga
    38101 gttaataggg aatccagtgc gaatctggag ctgacgcgca gcggtaagga aaggtgcgat
    38161 gattgcgtta tgcggacact gccatccggt gggaagtcat catctcttag tatcttagat
    38221 acccctccaa gcccgaagac ctgccggcca acgtcgcatc tggttctcat catcgcgtaa
    38281 tattgatgaa acctgcggca tccttcttct attgtggatg ctttacaatg attaaaaaag
    38341 cttcgctgct gacggcgtgt tctgtcacag ccttttccgc ttgggcacag gataccagcc
    38401 cggatactct cgtcgttact gctaaccgtt ttgaacagcc gcgcagcact gtgcttgcac
    38461 caaccaccgt tgtgacccgt caggatatcg accgctggca gtcgacctcg gtcaatgatg
    38521 tgctgcgccg tcttccgggc gtcgatatca cccaaaacgg cggttcaggt cagctctcat
    38581 ctatttttat tcgcggtaca aatgccagtc atgtgttggt gttaattgat ggcgtacgcc
    38641 tgaatctggc gggggtgagt ggttctgccg accttagcca gttccctatt gcgcttgtcc
    38701 agcgtgttga atatatccgt gggccgcgct ccgctgttta tggttccgat gcaataggcg
    38761 gggtggtgaa tatcatcacg acgcgcgatg aacccggaac ggaaatttca gcagggtggg
    38821 gaagcaatag ttatcagaac tatgatgtct ctacgcagca acaactgggg gataagacac
    38881 gggtaacgct gttgggcgat tatgcccata ctcatggtta tgatgttgtt gcctatggta
    38941 ataccggaac gcaagcgcag acagataacg atggtttttt aagtaaaacg ctttatggcg
    39001 cgctggagca taactttact gatgcctgga gcggctttgt gcgcggctat ggctatgata
    39061 accgtaccaa ttatgacgcg tattattctc ccggttcacc gttgctcgat acccgtaaac
    39121 tctatagcca aagttgggac gccgggctgc gctataacgg cgaactgatt aaatcacaac
    39181 tcattaccag ctatagccat agcaaagatt acaactacga tccccatttt ggtcgttatg
    39241 attcgtcggc gacgctcgat gagatgaagc aatacaccgt ccagtgggca aacaatgtca
    39301 tcgttggtca cggtagtatt ggtgcgggtg tcgactggca gaaacagact acgacgccgg
    39361 gtacaggtta tgttgagaat ggatatgatc aacgtaatac cggcatctat ctgaccgggc
    39421 tgcaacaagt cggcgatttt acctttgaag gcgcagcacg cagtgacgat aactcacagt
    39481 ttggtcgtca tggaacctgg caaaccagcg ccggttggga attcatcgaa ggttatcgct
    39541 tcattgcttc ctacgggaca tcttataagg caccaaatct ggggcaactg tatggcttct
    39601 acggaaatcc gaatctggac ccggagaaaa gcaaacagtg ggaaggcgcg tttgaaggct
    39661 taaccgctgg ggtgaactgg cgtatttccg gatatcgtaa cgatgtcagt gacttgatcg
    39721 attatgatga tcacaccctg aaatattaca acgaagggaa agcgcggatt aagggcgtcg
    39781 aggcgaccgc caattttgat accggaccac tgacgcatac tgtgagttat gattatgtcg
    39841 atgcgcgcaa tgcgattacc gacacgccgt tgttacgccg tgctaaacag caggtgaaat
    39901 accagctcga ctggcagttg tatgacttcg actggggtat tacttatcag tatttaggca
    39961 ctcgctatga taaggattac tcatcttatc cttatcaaac cgttaaaatg ggcggtgtga
    40021 gcttgtggga tcttgcggtt gcgtatccgg tcacctctca cctgacagtt cgtggtaaaa
    40081 tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact gcaggacggg
    40141 aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct ggtgtttgac
    40201 tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc ggatctccat
    40261 tacatttatg ctttcgataa cgtcgctttt ccgtatggcg aaaaaagcga agagtttatt
    40321 gttgagcgag tggtggcaat tgtcaccgca gtgcaagaac gttatcccct tgcgctggct
    40381 gtggtcgctt gcaacaccgc cagtaccgtt tcacttcctg cattacgcga aaagttcgac
    40441 ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac ggcaaatggc
    40501 attgtcggat tactggcaac ccgcggaact gttaaacgtt cttatactca tgagctgatc
    40561 gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat ggttgagttg
    40621 gctgaagcga agctacatgg cgaagatgtt tctctggatg cactaaaacg tatcctacgc
    40681 ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac ccatttccct
    40741 ctactacaag aagaactctt acaggtgctg ccagagggaa cccggctggt ggattctggc
    40801 gcagcgattg ctcgcagaac ggcctggctg ttagaacatg aagctccgga tgcaaaatct
    40861 gccgatgcga atattgcctt ttgtatggca atgacgccag aagctgaaca attattgccc
    40921 gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg ctgatttggt
    40981 tgaatg
//
LOCUS       DAHBHC010000042        40738 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHBHC010000042 DAHBHC010000000
VERSION     DAHBHC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.042"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..80
                     /locus_tag="M2A35_002626"
     CDS             <1..80
                     /locus_tag="M2A35_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCB7198911.1"
                     /translation="GEGVTSARTFVRLRRCVLVDAFRIV"
     gene            91..927
                     /gene="eutJ"
                     /locus_tag="M2A35_002627"
     CDS             91..927
                     /gene="eutJ"
                     /locus_tag="M2A35_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCB7198912.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            917..2104
                     /gene="eutG"
                     /locus_tag="M2A35_002628"
     CDS             917..2104
                     /gene="eutG"
                     /locus_tag="M2A35_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCB7198913.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2221..3447
                     /gene="eutH"
                     /locus_tag="M2A35_002629"
     CDS             2221..3447
                     /gene="eutH"
                     /locus_tag="M2A35_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCB7198914.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGA
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3444..4847
                     /gene="eutA"
                     /locus_tag="M2A35_002630"
     CDS             3444..4847
                     /gene="eutA"
                     /locus_tag="M2A35_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCB7198915.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4859..6220
                     /gene="eutB"
                     /locus_tag="M2A35_002631"
     CDS             4859..6220
                     /gene="eutB"
                     /locus_tag="M2A35_002631"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCB7198916.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6241..7128
                     /gene="eutC"
                     /locus_tag="M2A35_002632"
     CDS             6241..7128
                     /gene="eutC"
                     /locus_tag="M2A35_002632"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCB7198917.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7138..7797
                     /gene="eutL"
                     /locus_tag="M2A35_002633"
     CDS             7138..7797
                     /gene="eutL"
                     /locus_tag="M2A35_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCB7198918.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7810..8310
                     /gene="eutK"
                     /locus_tag="M2A35_002634"
     CDS             7810..8310
                     /gene="eutK"
                     /locus_tag="M2A35_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCB7198919.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8356..9408
                     /gene="eutR"
                     /locus_tag="M2A35_002635"
     CDS             8356..9408
                     /gene="eutR"
                     /locus_tag="M2A35_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCB7198920.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRMLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9414..10313)
                     /gene="hemF"
                     /locus_tag="M2A35_002636"
     CDS             complement(9414..10313)
                     /gene="hemF"
                     /locus_tag="M2A35_002636"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCB7198921.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10317..11186)
                     /gene="amiA"
                     /locus_tag="M2A35_002637"
     CDS             complement(10317..11186)
                     /gene="amiA"
                     /locus_tag="M2A35_002637"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCB7198922.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11400..11825
                     /locus_tag="M2A35_002638"
     CDS             11400..11825
                     /locus_tag="M2A35_002638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCB7198923.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11812..12261
                     /locus_tag="M2A35_002639"
     CDS             11812..12261
                     /locus_tag="M2A35_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCB7198924.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRSCFNEVKE"
     gene            12322..12897
                     /locus_tag="M2A35_002640"
     CDS             12322..12897
                     /locus_tag="M2A35_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCB7198925.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            12993..13892
                     /gene="yfeX"
                     /locus_tag="M2A35_002641"
     CDS             12993..13892
                     /gene="yfeX"
                     /locus_tag="M2A35_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCB7198926.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13950..15251)
                     /gene="pbp4b"
                     /locus_tag="M2A35_002642"
     CDS             complement(13950..15251)
                     /gene="pbp4b"
                     /locus_tag="M2A35_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCB7198927.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(15256..16680)
                     /gene="murP"
                     /locus_tag="M2A35_002643"
     CDS             complement(15256..16680)
                     /gene="murP"
                     /locus_tag="M2A35_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCB7198928.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16684..17580)
                     /gene="murQ"
                     /locus_tag="M2A35_002644"
     CDS             complement(16684..17580)
                     /gene="murQ"
                     /locus_tag="M2A35_002644"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCB7198929.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17744..18601
                     /gene="murR"
                     /locus_tag="M2A35_002645"
     CDS             17744..18601
                     /gene="murR"
                     /locus_tag="M2A35_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCB7198930.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18730..19521
                     /gene="ucpA"
                     /locus_tag="M2A35_002646"
     CDS             18730..19521
                     /gene="ucpA"
                     /locus_tag="M2A35_002646"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCB7198931.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19692..20708
                     /gene="cysP"
                     /locus_tag="M2A35_002647"
     CDS             19692..20708
                     /gene="cysP"
                     /locus_tag="M2A35_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCB7198932.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20708..21541
                     /gene="cysT"
                     /locus_tag="M2A35_002648"
     CDS             20708..21541
                     /gene="cysT"
                     /locus_tag="M2A35_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCB7198933.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21541..22416
                     /gene="cysW"
                     /locus_tag="M2A35_002649"
     CDS             21541..22416
                     /gene="cysW"
                     /locus_tag="M2A35_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCB7198934.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            22406..23503
                     /gene="cysA"
                     /locus_tag="M2A35_002650"
     CDS             22406..23503
                     /gene="cysA"
                     /locus_tag="M2A35_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCB7198935.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVELQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23637..24548
                     /gene="cysM"
                     /locus_tag="M2A35_002651"
     CDS             23637..24548
                     /gene="cysM"
                     /locus_tag="M2A35_002651"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCB7198936.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24551..24919)
                     /locus_tag="M2A35_002652"
     CDS             complement(24551..24919)
                     /locus_tag="M2A35_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCB7198937.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25024..25875
                     /gene="pdxK"
                     /locus_tag="M2A35_002653"
                     /gene_synonym="thiJ"
     CDS             25024..25875
                     /gene="pdxK"
                     /locus_tag="M2A35_002653"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCB7198938.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25917..26426)
                     /gene="crr"
                     /locus_tag="M2A35_002654"
     CDS             complement(25917..26426)
                     /gene="crr"
                     /locus_tag="M2A35_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCB7198939.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26467..28194)
                     /gene="ptsI"
                     /locus_tag="M2A35_002655"
     CDS             complement(26467..28194)
                     /gene="ptsI"
                     /locus_tag="M2A35_002655"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCB7198940.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28239..28496)
                     /gene="ptsH"
                     /locus_tag="M2A35_002656"
     CDS             complement(28239..28496)
                     /gene="ptsH"
                     /locus_tag="M2A35_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCB7198941.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28880..29851)
                     /gene="cysK"
                     /locus_tag="M2A35_002657"
     CDS             complement(28880..29851)
                     /gene="cysK"
                     /locus_tag="M2A35_002657"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCB7198942.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAQGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30036..30797)
                     /gene="cysZ"
                     /locus_tag="M2A35_002658"
     CDS             complement(30036..30797)
                     /gene="cysZ"
                     /locus_tag="M2A35_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCB7198943.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31027..32013
                     /gene="zipA"
                     /locus_tag="M2A35_002659"
     CDS             31027..32013
                     /gene="zipA"
                     /locus_tag="M2A35_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCB7198944.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32084..34099
                     /gene="ligA"
                     /locus_tag="M2A35_002660"
     CDS             32084..34099
                     /gene="ligA"
                     /locus_tag="M2A35_002660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCB7198945.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34101..34319
                     /locus_tag="M2A35_002661"
     CDS             34101..34319
                     /locus_tag="M2A35_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCB7198946.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34316..35314)
                     /locus_tag="M2A35_002662"
     CDS             complement(34316..35314)
                     /locus_tag="M2A35_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCB7198947.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            35404..36330
                     /locus_tag="M2A35_002663"
     CDS             35404..36330
                     /locus_tag="M2A35_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7198948.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36369..37133)
                     /locus_tag="M2A35_002664"
                     /pseudo
     CDS             complement(36369..37133)
                     /locus_tag="M2A35_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37382..38215
                     /gene="xapA"
                     /locus_tag="M2A35_002665"
     CDS             37382..38215
                     /gene="xapA"
                     /locus_tag="M2A35_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HCB7198949.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38275..39531
                     /gene="xapB"
                     /locus_tag="M2A35_002666"
     CDS             38275..39531
                     /gene="xapB"
                     /locus_tag="M2A35_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HCB7198950.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNSEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            39584..39742
                     /locus_tag="M2A35_002667"
     CDS             39584..39742
                     /locus_tag="M2A35_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HCB7198951.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMQ"
     gene            39783..40667
                     /gene="xapR"
                     /locus_tag="M2A35_002668"
     CDS             39783..40667
                     /gene="xapR"
                     /locus_tag="M2A35_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HCB7198952.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT         9511 a        10233 c        11278 g         9716 t
ORIGIN      
        1 ccggtgaagg ggtaaccagc gcgcgtacgt ttgtccgtct gcgtcgctgt gtattagtcg
       61 atgcgtttcg cattgtttaa ggagtaacag atggcgcacg acgaacaatg gctcacccca
      121 cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg ccgcgacgga atctccgctg
      181 tggctggggg tggatctggg cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt
      241 cagccggtgg cggtatgcct cgactgggcc gatgtggtcc gcgacggcat cgtctgggat
      301 ttcttcggcg ctgtcaccat tgttcgtcgc catctcgaca cgctcgagca gcaattcggt
      361 cgccgtttta gccatgcggc gacttcattt cctccaggta cggacccgcg tatttccatc
      421 aacgtgctgg agtctgccgg gctggaagtg agccatgtgc tggatgagcc aaccgcagtc
      481 gcggatctac tgcaactgga taacgcaggc gtagtggata tcggcggtgg cactaccggc
      541 attgccatcg tgaaaaaggg caaagtgacg tactcggcgg atgaagccac cggcggacat
      601 cacatctctc tgactctcgc cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac
      661 aagcgcggtc acggtgacga gatttggcct gcggtgaaac cggtgtacga aaaaatggcg
      721 gacattgtcg cccggcatat tgaagggcag ggcattactg atttatggct ggcgggcggc
      781 tcctgtatgc aaccgggcgt ggcagaactg tttcgcaaac aattcccggc gttacaggtg
      841 catttaccgc agcacagttt gtttatgacc ccgctggcga tcgccagtag tgggcgagag
      901 aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac
      961 cctgaatctg caacgggtaa aaacatttag cgttccaccg gtgacgcttt gcggtctggg
     1021 cgcggtgagc agttgcgggc agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat
     1081 ggcagacagc tttttgcatc aggcagggat gaccgccggg ctgacgcgca gcctggccgt
     1141 taaaggcatc gccatgacgc tctggctatg tccggtgggc gaaccgtgca ttaccgacgt
     1201 gtgtgcagcc gtggcgcagt tgcgtgagtc aggttgtgac ggggtgatcg catttggcgg
     1261 cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac
     1321 gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc ttgccgctga ttgccattcc
     1381 aactaccgcc ggaaccggct ctgaaaccac caatgtaacg gtgattatcg acgcggtgag
     1441 cgggcgcaag caggtgttag cccatgcctc gctgatgccg gatgtggcga tcctcgacgc
     1501 cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg accggcattg atgcgttaac
     1561 ccatgccatt gaagcataca gcgccctgaa cgctacaccg tttaccgaca gtctggcgat
     1621 tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg gtgggctacg gtcacgacct
     1681 tgccgcgcgc gagagcatgt tgctggcttc atgtatggcg ggaatggcgt tttccagtgc
     1741 gggtcttggg ttgtgccacg cgatggcgca tcagccgggc gcggcgctgc atattccaca
     1801 cggtctggcg aacgccatgt tgctgccaac ggtgatggaa tttaaccgga tggtttgtcg
     1861 cgaacggttt agtcagattg gtcgggcact gcgaactaaa aaatccgacg atcgtgacgc
     1921 tattaacgcg gtaagtgagc tgattgcgga agttgggatt ggtaaacgac tgggcgatgt
     1981 tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc gcgcaggaag atatttgtct
     2041 gcgcagcaac ccgcgtaccg ccagcctgga acagattgtc ggactgtacg cggcggcgca
     2101 ataaatgccg gatgcgacgc ttgccgcgtc ttatccggcc tacgggattg cacatgtagg
     2161 gcggataagg cgtttacgcc gcatccgcca ataaataaca actaacaggg agtaaaggcg
     2221 atgggaatta acgaaatcat catgtacatc atgatgttct ttatgctgat agctgccgta
     2281 gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc tcggtaagtt cggtaaaagt
     2341 atcgaaggag caggcggtca gttcgaagaa ggctttatgg caatgggcgc tctgggtctg
     2401 gcgatggtcg gtatgaccgc gctggcaccg gtactggctc acgtactcgg gccggtaatt
     2461 atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt tcgccggaac actgctggcg
     2521 tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg gcggcgacgt agcagcgtgg
     2581 ctatactctg ggttaattct cgggtcgatg atggggccaa cgattgtgtt ttccattccg
     2641 gtggcgctcg gcattatcga accttctgat cgtcgttatc tggcgctcgg cgtgctggcg
     2701 ggcattgtga ccattccgat tggctgtatt gctggtggtc tggttgctat gtactccggt
     2761 gtgcagatca acggtcagcc ggtggaattc actttcgccc tgatcctgat gaacatgatc
     2821 ccggtgatca ttgtcgcgat tctggtggcg ctggggctga aattcatccc ggaaaaaatg
     2881 atcaacggct tccagatctt tgccaaattc ctcgttgcat tgatcaccct cggtcttgcc
     2941 gctgcggtag tgaaattcct ccttggctgg gaactgatcc ccggactgga tcctatcttt
     3001 atggcccctg gcgataaacc cggtgaagtg atgcgcgcca ttgaagttat cggttctatc
     3061 tcctgcgtgc tgttaggggc gtatccaatg gtgctgctgc tgactcgctg gtttgaaaaa
     3121 ccgctgatga gcgtcggtaa agtactgaat atgaacaaca tcgcggcagc cggcatggtg
     3181 gcaacgcttg ccaacaacat cccgatgttc ggcatgatga agcagatgga tacccgcggc
     3241 aaagtcatca actgcgcctt cgccgtttcc gctgctttcg ccctgggcga ccacttaggc
     3301 ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga ttgtcggcaa gttgatcggc
     3361 ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa aagaagacgc gaccgcgact
     3421 aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct attgagcgtc ggtatcgata
     3481 tcggcaccac caccacccag gtgattttct cccacctgga actggttaac cgtgcggcgg
     3541 tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat tagctggcaa agcccggtat
     3601 tctttacccc tgtcgataaa cagggtggtt taaaagaagc ggaactgaaa tcgctgattt
     3661 tagagcaata tcaggctgcg ggcattgcgc cggaaagcgt tgattctggt gccatcatca
     3721 tcaccggtga aagcgcgaaa acccgcaatg ctcgcccggc ggtgatggcg ctctctcagt
     3781 cgctgggcga ttttgtcgtt gccagcgccg ggccgcacct cgaatccgtg atcgccggtc
     3841 acggagccgg ggcacaaacc ctttctgaac aacggctgtg tcgggtactg aatatcgaca
     3901 tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct
     3961 gtctcaacgt cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg gtttacgctc
     4021 ataaaccggg gcagatgatt gtggatgagt gcttcggtgc aggcactgac gcccgttcgc
     4081 tgaccggcgc gcagctggtg caggttaccc ggcggatggc ggcgctgatt gtcgaagtga
     4141 ttgacggaac gctttcgccg ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag
     4201 gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg cgaatgttat cgccaccagc
     4261 ccgccgaccc gttctgtttt gccgatattg gcccacttct ggcaacggcg ctgcatgacc
     4321 atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca aaccgtacgc gccacggtga
     4381 ttggcgcggg tgcacatacc ctttcgctct ctggcagcac aatctggctg gagggcgtac
     4441 aactgccgct gcgcaatttg ccggtggcga tcccgattga tgaaacggat ctggtgagtg
     4501 cctggcaaca ggcgctgctt cagctggatc ttgatcccaa aactgacgcg tacgtgctgg
     4561 cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc aacgcgctgg
     4621 tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc gggcaggact
     4681 ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca acaactcccg ttggcagtca
     4741 ttgacgaagt gattgtccgc gcgggggact atatcgacat tggtacgcct ctttttggcg
     4801 gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc ttcctgaggg aacgacttat
     4861 gaaactaaag accacattgt tcagcaatgt atatcagttt aaggatgtaa aagaggtgct
     4921 ggctaaagcc aacgaactgc gttcggggga tgtgctggca ggcgttgcag cggcaagttc
     4981 acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg accgtagcgg acatccgcaa
     5041 taatccggtg attgcctatg aagatgactg cgtgacgcgg ctgattcagg acgatgttaa
     5101 cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc gaactgcgtg agtatgtgct
     5161 gagcgatgaa accagcgtgg acgacattgc ctttacccgc aaagggctga cctcggaagt
     5221 ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg atctacggcg cgaagaaaat
     5281 gccggtaatc aaaaaggcca ataccaccat tggtattccg ggcaccttca gcgcccgttt
     5341 gcagccaaat gacacccgtg acgacgtgca aagtatcgcc gcgcaaatct acgaagggct
     5401 ttccttcggg gtgggcgatg cggtgatcgg cgttaacccg gtgactgacg acgtggaaaa
     5461 cttaagccgc gtgttggaca ccatttacgg cgtgatcgac aaattcaaca tcccaactca
     5521 gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg attcgtcgcg gcgcgcctgg
     5581 cggactgatt ttccagagta tctgtggcag cgaaaaaggg ctgaaagagt ttggcgtgga
     5641 actggcgatg ctcgacgaag cgcgcgcagt gggtgcagag ttcaaccgta tcgccgggga
     5701 aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta tccgctggcg ctaacttcgg
     5761 cgctgaccag gtgacgatgg aagcacgtaa ctatgggctg gcgcgtcatt acgatccgtt
     5821 tatcgtcaac accgtggtcg gctttattgg gccggagtat ctctacaacg accgccagat
     5881 tatccgtgct ggcttagaag atcactttat gggcaagctg agcggcatct ctatgggctg
     5941 tgactgctgt tacaccaacc acgctgacgc tgaccagaac ctcaacgaaa acctgatgat
     6001 cctgctcgcc accgcaggct gcaactacat catggggatg ccgctgggtg atgacatcat
     6061 gctcaactac cagaccaccg cattccacga taccgccact gtgcgtcagt tactcaacct
     6121 gcgtccgtca ccggagtttg aacgctggct ggaaagcatg ggcattatgg caaacggtcg
     6181 cctgaccaaa cgggcgggcg atccgtcact gttcttctga tgacgcggag ataacacatc
     6241 atggatcaaa aacagattga agaaattgta cgcagcgtga tggcgtcaat gggacaaacg
     6301 gcccccgcgc cgtcagaagc aaagtgcgcc accaccaact gtgcggcacc ggtgacctcg
     6361 gaaagctgtg cgctggattt aggttccgct gaagcaaaag cgtggattgg tgttgaaaac
     6421 ccgcatcgcg cagacgtatt aacagaactg cgccgcagca ccgtggcccg cgtttgtacc
     6481 ggtcgtgccg gtccgcgtcc acgtacccag gcgctgctgc gtttcctggc cgatcactct
     6541 cgttcgaaag ataccgtact gaaagaagtg ccggaagagt gggtgaaagc gcagggcttg
     6601 ctggaagtac gctctgaaat tagcgacaaa aatctctacc tgactcgccc ggatatgggc
     6661 cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac agtgtgttgc taatccggac
     6721 gtacaggtcg ttatttctga tggcctgtca actgatgcca tcaccgtgaa ctacgaagag
     6781 atcctgccgc cgctgatggc gggcctgaaa caggccgggc tgaaagtcgg cacgccattc
     6841 tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg gtgagatcct cggcgcgaaa
     6901 gtggtgatcc tgctggtggg ggaacgtccg gggttagggc agtcagaaag cctctcctgc
     6961 tacgccgttt actcgccgcg tatggcgacc accgttgagg ccgatcgcac ctgtatctct
     7021 aacattcacc agggcggcac gccgccggtt gaagccgcag ccgtcattgt ggatttggcg
     7081 aaacgtatgc tggagcagaa agcatccggc atcaacatga cccgataagg aggcatcatg
     7141 ccagctttag atttgattcg accgtcggta accgccatgc gggtgattgc ctccgttaac
     7201 gctgaatttg cgcgtgaact aaaattgccg ccgcatattc gtagcctcgg gctgatttct
     7261 gctgattccg atgacgtcac ctacattgcc gctgatgaag cgaccaagca ggcgatggtg
     7321 gaagtggtat atggtcgctc gctgtatgcg ggcgctgcac acggcccgtc accgactgcc
     7381 ggtgaagtgc tgattatgct tggtgggcca aacccggcgg aagtgcgtgc cggtctggat
     7441 gcgatggttg cacatattga aaatggtgca gcattccagt gggccaatga cgcagaaaac
     7501 accgccttcc tggcgcatgt ggtttcgcgt accggttctt atctctcatc aaccgccggg
     7561 atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc cgctggaagc gacctatggc
     7621 attgatgcag cgttgaaatc tgccgacgta cagctggtga cctatgtccc gccaccgtct
     7681 gaaaccaact actcggcagc atttttaacc ggtagccagg ccgcgtgtaa agcagcctgt
     7741 aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc caatccagcg tgcgtaacgg
     7801 aggttgccga tgatcaatgc actgggattg ctggaagtgg acggcatggt cgccgcgata
     7861 gatgcagcgg atgccatgct caaagcagct aacgttcgtc tgctcagtca cgaagtgctt
     7921 gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg cggcgtgtcg tgcggcgctg
     7981 gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca tcagccgcaa ggagatcggt
     8041 cggccagacg atgacaccca gtggctggtt actggtttta accgccagcc gaagcaaccc
     8101 gtaagggaac ccgacgcgcc agttatcgtc gcggaatctg ctgacgagtt gttggcgctg
     8161 ttaacatcag tacgtcaggg aatgacggca ggagaagtgg ctgcccactt tggctggccg
     8221 ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg ccgggacgtt acgtaaacgc
     8281 agtagtcgtt atcgtctcaa gccccattaa cctgtcggag gtgccgggtg tcctacaaca
     8341 cccggcatta acatcatgaa aaagacccgt acagccaatt tgcaccatct ttatcatgaa
     8401 cccttacccg aaaacctgaa gctcacgccg aaggtcgaag tggataatgt tcatcaacga
     8461 cagacaacgg atgtctatga acatgcttta acgattaccg cctggcagca gatttacgat
     8521 cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa ttctactcga tgatattcag
     8581 gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt gcctggtctg gccgaactcg
     8641 ttctggtttg gcattccggc gacgcgcggt gagcagggat ttatcggttc gcaatgtctg
     8701 ggaagcgcgg aaatcgccac ccgccctggt ggcactgaat ttgaactgag cacgccggat
     8761 gattacacga tcctgggcgt ggtgctttct gaagatgtca tcacccggca ggctaacttt
     8821 ttgcataacc cggatcgggt attacatatg ctgcgtagcc agtcggcgct ggaagtgaaa
     8881 gagcagcata aagccgcgct gtggggcttt gtccaacagg cgctggcgac gttttgcgag
     8941 aacccggaaa atctccatca gcctgcagtg cgaaaagtgc tgggggataa tttgctaatg
     9001 gcgatggggg cgatgctgga agacgcgcaa ccaatggtga cggcggaaag catcagtcat
     9061 cagagttacc gtagattact ttcccgcgcc cgtgaatatg tgctggaaaa tatgtctgag
     9121 ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca gtcgccgcat gctacaaaac
     9181 gcgtttcacg ctattttagg cattggccca aacgcgtggc tgaaacgcat tcgcctgaac
     9241 gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca caacggtaaa agacgccgcc
     9301 atgcagtggg gattctggca tctggggcaa tttgccacgg attaccagca gctgtttgcc
     9361 gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat gggggtgagg gagttacacc
     9421 caatccctga ccttaataaa ctcacttaac gccgcttctg ggctgccatc ttttggctga
     9481 taatcatact cccagcgtac cagtggcggc attgacatca ggatcgactc ggtgcgcccg
     9541 ccagtttgca ggccaaacag agtgccgcga tcccagacca gattgaactc gacataacga
     9601 ccgcgacggt agagctgaaa attgcgctcg cgctcgccgt aggccatcgc tttacgtcgc
     9661 tctacaattg gtaaataagc gtcggtgtag cctttgccta ccgcctgcat aaaggcaaaa
     9721 cagtggtcga aatctggcgt gttcagatca tcaaagaaca gcccgccaat accgcgctgt
     9781 tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact ttttgtaacg gggataaacg
     9841 tcttcaccaa atggcaggca caggtcacgg gcggtgcggt gccagtgaat ggcgtcttct
     9901 tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa accaccagac gggctctgca
     9961 cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc tggtgggaac atacgggtta
    10021 tgcggatgca ctaccagtga aacgcccatc gcctcgaaac tgcgcccggc aagttccggg
    10081 cgatgagcgg tggcggaagc tggcatcgcc tcgccgtgga cgtgagaaaa gttgacgcct
    10141 gcctgttcga aaacaccacc attacgcaac acccgactac gcccgccgcc gccagcttcg
    10201 cgctgccaac tatcttcgac aaattctgcg ccatcgacgg cggtcagctg ctgacaaatc
    10261 gtatcctgaa ggttgagcag aaactgttta acctggtgtg cgtcgggttt cataacttat
    10321 cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga aataactgat cacgccttca
    10381 gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc ctaacagccg ctcttcttcc
    10441 gggttggtga taaacgaggt ttccaccagc accgaaggaa ccgacggcga tttcaacacc
    10501 acaaacgcgg cttgttcggt gttgcggctg tgcagtttat gcaccggctt aatctttttc
    10561 agaatatgcg agccgagcgt caggctgttt ttaatagtat cggtttgcac cagatcaaac
    10621 agcacttgct gcaataggtg atccttgtca gtcgcctttt taccggcaac ttcatcggcg
    10681 cggttttcgc gttcagacag gtatttcgcc attgcgctac ttgccccacg gttagagagg
    10741 gcaaataccg aagcaccggc agctttcggg ttggtaaagc catcggcatg aattgacata
    10801 aacagatctg cgccatgttt atgggcgatt tcaacgcgat cgtaaagtgg gataaacgta
    10861 tcgccagaac gcgttaaacg cgcatcaatc ccatgattac gcaaaatgga acggacgttt
    10921 ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt tgcgtccgat cgctccggta
    10981 tcaattccgc cgtggcctgg atcgagaaca acgacacgtt tgccgccaga ttttttggct
    11041 ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt ctttggcgat ggcttgcgac
    11101 attcctgaca acgtcagggc agccaaaccg gctttcagca cctggcggcg cgaagtgagt
    11161 gtttttagtg gtttaaaagt gctcatacgg cctgagttgt aataataaag ttccagatgt
    11221 tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg agaattgttt tacttttcat
    11281 ttcaatagtt gacaactccc cattatgcca ctttttcaac cgattttcgt cagatattgg
    11341 ctaattcgac cgttcacgcc ataatcagcc ttttaacccg caaaaaggta acgaatacca
    11401 tggagatacg cgtatttcgc caggaagatt tcgaagaggt catcaccctt tgggagcgtt
    11461 gcgacttgct gcgtccgtgg aacgatccgg aaatggacat cgagcgtaag atgaaccatg
    11521 acgtcagttt gtttttggtc gctgaggtaa acggtgaagt ggtcggaacg gtgatgggcg
    11581 gttatgacgg gcatcgcggg tctgcttatt atcttggcgt gcatccagag tttcgtgggc
    11641 gtgggattgc caatgcgttg cttaatcggc tggagaaaaa gctgattgct cgtggctgcc
    11701 cgaaaattca gatcaatgtg ccggaagata acgacatggt gctcggaatg tatgaacgcc
    11761 tgggatatga acacgccgac gtgctgagtc tgggtaagcg tttgattgaa gatgaagagt
    11821 actgagttcc atcctgtcca ttatgatgcg cacggacgcc tgcgtttacc cttgctcttc
    11881 tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt ttgtcattgc cggtgcgtcg
    11941 cgtgagcagg gtactgcgct gctaaatctg ttttatcccg atcacgataa tttctggctg
    12001 gggctgattc ctggcattcc ggcggtgctg gcgtttttgt tgagcggtcg gcgggctaca
    12061 tttcctcgca cttggcatgt gctatatttt ctgttgctgt tggcgcaggt ggttttactt
    12121 tgctggcaac cgtggctgtg gctgaacggt gaatccgtta gcggtatcgg tctggcgctg
    12181 gtagtggcgg atattgtggc gttaatctgg ctgctgacca atcgacgttt acgctcttgt
    12241 tttaatgaag taaaagaata acggcacttt ttggtgaatt tgcactccaa gcaacgttat
    12301 tgaataacca aaggaagtga catgaaatcg ctgcgtttaa tgttatgcgc tatgccattg
    12361 atgctgaccg gctgttccac gatgtcgtca gttaactggt ctgccgctaa cccgtggaac
    12421 tggtttgggt cgtccaccaa agtgagcgag cagggcgttg gtgaattaac ggcgtccaca
    12481 ccactgcaag aacaagccat tgccgatgcg cttgatggcg attatcgcct gcgcagcgga
    12541 atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag tgatgaaggg cgacaacgtg
    12601 gcgatggtga ttaacggcga tcaaggcacg atcagtcgca ttgacgtgct ggatagcgat
    12661 attcctgctg acaccggtgt taaaatcggt acaccgttta gcgaccttta cagcaaagca
    12721 tttggtaatt gccaaaaagc tgatggtgat gataatcgtg ccgtcgaatg taaagccgaa
    12781 ggcagtcaac atattagcta ccagttcagc ggggaatgga gtggtcctga agggttaatg
    12841 ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa ttatctggcg gcgttaattt
    12901 gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat agcgctcaac aatgccacgg
    12961 attgcgtggc attattattt tcaggaggaa caatgtctca ggttcagagt ggcattttgc
    13021 cagaacattg ccgcgcggcg atttggatcg aagccaacgt gaaaggggaa gttgacgccc
    13081 tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac ttttgaagcg aaattcccgg
    13141 acgcgcatct tggtgcggtg gttgcctttg gtaacaacac ctggcgcgct ctgagcggcg
    13201 gcgttggggc agaagagctg aaagattttc cgggctacgg taaaggcctt gcgccgacca
    13261 cccagttcga tgtgttgatc cacattcttt ctctgcgtca cgacgtaaac ttctctgtcg
    13321 cccaggcggc gatggaagcc tttggtgact gcattgaagt gaaagaagag atccacggct
    13381 tccgttgggt tgaagagcgt gacctgagcg gctttgttga cggtacggaa aacccggcgg
    13441 gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg cgtggatgcg ggcggcagct
    13501 atgtgtttgt ccagcgttgg gaacacaacc tgaagcagct caaccggatg agcgttcacg
    13561 atcaggagat gatgatcggg cgcaccaaag aggccaacga agagattgac ggcgacgaac
    13621 gtccggaaac ctctcacctg acccgcgttg atctgaaaga agatggcaaa ggactgaaga
    13681 ttgttcgcca gagcctgccg tacggcaccg ccagtggcac tcacggtctg tacttctgcg
    13741 cctactgcgc gcgtctgcat aacattgagc agcaactgct gagcatgttt ggcgataccg
    13801 atggcaagcg tgatgcgatg ttacgtttca ccaaaccggt cactggcggc tattacttcg
    13861 cgccgtcgct ggacaagttg atggcgctgt aagcgtttct gattcgatga tgaaaggcca
    13921 ggcagtaaca ttactgactg gcctttttat tactgcttta acgccgcata tacctgatca
    13981 accacccaac cataagttgc aattggcaac tgaccgcttt cgaacatatt gggattcttt
    14041 tgcggatcgg caaccggtga atgtggcttg ttacttaaca tcacaatcgc catatgattc
    14101 accggatcga taacggtcac cgttccggtc cagccagtgt gaccgtaggt ttgcgggctt
    14161 gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat ttacgcgcca gccgaggcca
    14221 aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc actgaacagc
    14281 tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc aatatcgccg
    14341 gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa ggctttttca
    14401 tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat cacgccatcg
    14461 cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc tttcagcagc
    14521 gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc aacatagcgg
    14581 tcaagcggct gaccggtgac cgactcgacg ataaatccaa gcagcatata atcgacatca
    14641 ctgtaaatat gcttgctgcc gggctgatac tctagcggcg tgcgcttgat catctccagc
    14701 gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg gtattgcgga
    14761 tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt gtttttgcct
    14821 ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat ccgatcgtca
    14881 ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc gtacattttg
    14941 gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg ttccatcaac
    15001 acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat ctgattatct
    15061 ttaatgatca gcaggtttac gccgggataa ccggcgtcca cttgctggct aatccagcga
    15121 tccatctggt taagccgttc gacgttgaac cctgctttct ctggcgaact ttctgtcaga
    15181 acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag atagagcatt
    15241 gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg tggtgaaaat
    15301 aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccattg ccggaaggat
    15361 gccttgtgcg gaagtcatca gcggcagcgc caccagacca gacggcccaa aggcgctgtt
    15421 taagcccatc ggcagacccc accaggctat caggccgata aacaaaccgc ccgctgcgcc
    15481 gcctaaacag gcggtaataa acggtttcat gcggggcagg gtgacgccgt aaatcaacgg
    15541 ttcaccaacg cccagcagac cgggaataat cgccccgcgt acctgactgc gtaatgcact
    15601 gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg cgcctgccat
    15661 tgaaaggatg ggaaataagc tgttgaatcc ctggctgtcc attaacgcga ggtaaacagg
    15721 aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg ctaaaaccgc
    15781 acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt cgaataacca
    15841 gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca gggtgattaa
    15901 cgaggtcagc agcatgtcga gatcgtccgg cataaagcgg cgcaccatgc cttcaatgcg
    15961 ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat cgatgggcag
    16021 accaaagaaa tcgtgaaaac cggcgtagta accggtggtt gcagcggggt tataaccgag
    16081 caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg cctgggcggc
    16141 gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca ccttcataaa
    16201 attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca ccgtggcaat
    16261 taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca gcggcgtgaa
    16321 gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg cttttaactg
    16381 acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc ccagcagctc
    16441 gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct gcacctggtc
    16501 gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat caaccagtga
    16561 actgtcatgt acacccagac gcaggcgcgt catacagtta ccacaactgg cgatatttcc
    16621 cggtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct ctttggccat
    16681 gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca ggcgtttttt
    16741 agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac agttgcgctc
    16801 gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt ttttaacgat
    16861 attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca tcaggttgcc
    16921 gaacactttg ccggatttaa tcatcagccc ggtggagagc atattgagca ccagtttctg
    16981 cgctgtacct gctttcatcc gcgaagaacc ggtaaccact tcggcaccta caatcggtgt
    17041 aatggcaaac tctgcggtgg ttgaaacggc gctccccgga ttacaggaaa ttcctactgt
    17101 gcggcagccg agctggcgtg cgtattccag tccggcaatt acatacggcg tgcgaccact
    17161 ggccgcaatg ccaaccacca catcctgcgc ggttaagccg atgtttttca gatcgttaac
    17221 accaccttcc cggctatctt cagcgccttc caccgcatgc tgaatggcat attcgcctcc
    17281 agcaatcaaa ccaaccacca aacccggttt cacgccgtag gtgggcggac attcgctggc
    17341 atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac gaccgccgcc
    17401 gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagta cgcgctcaac
    17461 ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca gcgttgatac
    17521 gcggtcaatt tcagccgagg cggcgttcga gccttcggta atcatctttt caagttgcat
    17581 cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc atatatcccg
    17641 aattgacaga cttaaaggaa tcattgagtc tacactcgca gagtgtgaag agcgtcataa
    17701 gaacttaagc ggaatttttg ctgccagccc aggagagtgg aagatgttgt acctgacgaa
    17761 aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg actttctgcg
    17821 ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac agctcggcat
    17881 cagccagtcg agtattgtga agtttgccca gaagctcggc gcgcaaggat ttactgaatt
    17941 gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg ccacggcgct
    18001 gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc gcaaactgaa
    18061 tcgcgaaaaa gagctggcgc tggagcaaac ctgcgcgttg ttcgactacg cgcggctgca
    18121 aaaaatcatt gatgtcatca gtaaagcgca atttattcag ataacgggcc tgggcggatc
    18181 ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc gcgtggcctg
    18241 cgaagccgac acgcacgtac aagccaccgt ttcccaggcg ttgaaaaaag gcgatgtaca
    18301 aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag aagcggcacg
    18361 aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc tgcggcggct
    18421 ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct cgtcgatgtc
    18481 tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg tgcaactcaa
    18541 tgatgtcgag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac gcctgaaatg
    18601 atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat ccgcagcaat
    18661 attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat taacaaggag
    18721 agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc attgcaggga
    18781 attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat cttgctggat
    18841 atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca tcgctgtacg
    18901 gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa acgcgcgaag
    18961 gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg tctgggcagt
    19021 ttcctcgata tgagcgatga agatcgcgat ttccatattg acatcaatat taaaggcgta
    19081 tggaacgtca cgaaggcggt gctgccagag atgattgcgc gcaaagatgg tcgcattgtg
    19141 atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc gtacgcctta
    19201 acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc gcagtctggt
    19261 attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga aagcattgcc
    19321 cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa agcaatcccg
    19381 atgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct cgcatcggat
    19441 gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac actgccggag
    19501 acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg gggaggattt
    19561 cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg gtatataaaa
    19621 ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca ttaaatttat
    19681 aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgtgctg gtcgcttctc
    19741 tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat gacgtctccc
    19801 gcgagctgtt tgccgccctg aatccgccgt ttgagcaaca atgggcaaaa gataacggcg
    19861 gcgacaaact gacgataaaa caatctcatg ccgggtcatc aaaacaggcg ctggcgattt
    19921 tacagggctt aaaagccgac gttgtcactt ataaccaggt gaccgacgta caaatcctgc
    19981 acgataaagg caagctgatc ccggccgact ggcagtcgcg cctgccgaat aatagctcgc
    20041 cgttctactc caccatgggc ttcctggtgc gtaagggtaa cccgaagaat atccacgatt
    20101 ggaacgacct ggtgcgctcc gacgtgaagc tgattttccc gaacccgaaa acgtcgggta
    20161 acgcgcgtta tacctatctg gcggcatggg gcgcagcgga taaagctgac ggtggtgaca
    20221 aaggcaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg ttcgataccg
    20281 gcggtcgtgg cgcaaccacc acttttgccg agcgtggcct gggcgatgtg ctgattagct
    20341 ttgaatcgga agtgaacaac atccgtaaac agtatgaagc acagggcttt gaagtggtga
    20401 tcccgaaaac caatattctg gcggaattcc cggtggcgtg ggtcgataaa aatgtgcagg
    20461 ccaacggtac ggaaaaagcc gcaaaagcct acctgaactg gctctacagc ccacaggcgc
    20521 agaccatcat caccgactat tactatcgtg tgaataaccc ggaagtcatg gacaaactga
    20581 aaaacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc tcctggccgg
    20641 aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta gcggcggggc
    20701 gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct taagcctcgg
    20761 caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc tggtgatgca
    20821 gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc aggtagtagc
    20881 ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg gcgtttttgg
    20941 cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc tgcttgatgc
    21001 gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc tagcctcgct
    21061 cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca aagtcaccta
    21121 tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg tggtacgtac
    21181 cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcgg aaacgcttgg
    21241 tgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gagctttctc cggcgctggt
    21301 ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg tgatttttat
    21361 cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg tgcgcttaca
    21421 ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg catctctgct
    21481 gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg taggtcatta
    21541 atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg gggcaaatgg
    21601 tttctgattg gcatcgggat gctggtttcg gcgttcatcc tgctggtgcc gatgatttac
    21661 atcttcgtgc aggcattcag caaggggctg atgccggttt tacagaatct ggccgatccg
    21721 gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt accggtaaac
    21781 ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc tggacgccag
    21841 ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc cggtctggtg
    21901 tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga acataatctg
    21961 caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac gtgtccgttt
    22021 gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggagga tgaagcggcg
    22081 attttgcttg gcgcgtctgg ctggcagatg ttccgtcgcg tcacattgcc gaatattcgc
    22141 tgggcgctgc tttatggcgt ggtactgacc aacgcccgcg cgattggcga gtttggcgca
    22201 gtgtcggtgg tttccggctc gattcgcggc gaaacacttt cgctgccgtt acagattgaa
    22261 ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact gttaacgctg
    22321 atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga gaatcaggaa
    22381 aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta agaagtcgtt
    22441 tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc agatggtcgc
    22501 gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg ccgggctgga
    22561 gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc tgcacgcgcg
    22621 cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata tgacggtatt
    22681 cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gagcgcccga atgccgcagc
    22741 catcaaagcg aaagtgacaa aattgctgga aatggtccag cttgcgcatc tggcggatcg
    22801 ttatccggcg cagctttccg gcggccagaa acagcgtgtg gcgctggcgc gcgcgctggc
    22861 tgtagaactg caaattctgc tgcttgatga accgtttggc gcgctggatg cgcaggtgcg
    22921 taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca ccagcgtgtt
    22981 tgtgacccac gaccaggaag aggcgaccga agtagccgat cgtgtagtgg tgatgagtca
    23041 ggggaatatt gaacaggctg atgcgccgaa tcaggtatgg cgcgaaccag cgacccgttt
    23101 tgtactcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg gcgggcagtt
    23161 ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg ggccggtgga
    23221 tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg attcgccgct
    23281 gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag tggtgcagcc
    23341 gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg ccccgcagcg
    23401 tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg acgagcgtat
    23461 cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg agtgaatgtt
    23521 aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt gtaacctgta
    23581 gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata gagatcgtga
    23641 gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga atggggccgg
    23701 ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt tcggtgaaag
    23761 atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt aaaccgggtg
    23821 atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg attgccgcgc
    23881 tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa cgccgtgcgg
    23941 cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc atggaaggtg
    24001 cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc gatcagttta
    24061 ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc tggcagcaaa
    24121 ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact atcaccggcg
    24181 tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg cagccggaag
    24241 aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg gggattttca
    24301 acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa aacaccatgc
    24361 gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc gcggttgccg
    24421 gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc atctgcgatc
    24481 gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagc cagggggcgg
    24541 ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattagc gcatgtaaaa
    24601 aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc ttcccggtaa
    24661 tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg cggttacggg
    24721 tattgcccag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt tcatcaccgt
    24781 tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg ttgatgcggg
    24841 cttcttcatg ggcagttaac ttcgcgtaag cggggagtgt cattagtagt gtaatgacca
    24901 gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc gatttgactg
    24961 ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag gagaaaaaat
    25021 taaatgagta gtttgttgtt gtttaacgat aagagcaggg cgctgcaggc ggatatcgtc
    25081 gccgtgcagt cgcaggtggt ttacggcagc gtgggcaaca gcattgccgt gcctgctatc
    25141 aaacagaacg gtctgaatgt ctttgccgtg ccgacggtat tgctgagcaa tacgccgcat
    25201 tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta tttgcgtgcg
    25261 cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta tatgggaacg
    25321 gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga ccatcctgac
    25381 ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta tgtcaaacct
    25441 gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcagggaat tacccccaat
    25501 atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag tgccattgct
    25561 gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag cgcctccggt
    25621 aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt gaatgtcatt
    25681 tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg tgctcagctt
    25741 atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc ggggttgcgc
    25801 gtactggaag tgatgcgcta cacccagcag catgagagcg atgaattgat tttgccgccg
    25861 ctggcggaag cataaaaaat ggcgccgatg ggcgccattt ttcactgcgg caagaattac
    25921 ttcttgatgc ggataaccgg ggtttcaccc acggttacgc taccggacag tttgatcagt
    25981 tctttgattt cgtccatgtt ggagataaca accggagtca gggtagactt ggctttctct
    26041 tccagcagcg gcagatcaaa ttcaatgaca gtatcgccaa ctttcacgcg ctgaccttct
    26101 tcagcaatac gcttgaagcc ttcgcctttc agttcaacgg tgtcgatacc gaagtggacg
    26161 aacagttcaa cgccgctatc agattcgata gagaacgcgt ggttggtttc aaagatttta
    26221 ccaatggtgc cgtctacagg cgcgaccatt ttgttacccg ttggtttgat agcaatacca
    26281 tcaccaacga ttttttccgc aaaaacgaca tccggcacgt cttcgatatt gacgatctcg
    26341 ccagagagcg gagcaatgat ctcaatagtt ccggtatcct tcttgtcgtc ggaaaccaga
    26401 gatttcagtt tatcgaacaa acccatgatc ttctcctaag cagtaaattg ggccgcatct
    26461 cgtggattag cagattgttt tttcttcaat gaacttgtta accagcgtca ttaactcgtc
    26521 cgttgtcggt tgagcaagag cctgctctgc taacaccttc gcatcttcga agttcgtgtt
    26581 acggataatc ttcttaatgc gcgggataga aatggcgctc atagagaatt cgtccagacc
    26641 catccccagc aacagaagtg tagcacgttc atcgccagca agctcaccac acatgccagt
    26701 ccatttgcct tcagcatgag aagcatcaat aacttgcttg atcaagttca gcacggacgg
    26761 tgacattggt tggtaaaggt gtgaaatcat atcattacca cggtcaactg ccagagtgta
    26821 ctgcgttaaa tcattggtgc cgatactaaa gaaatcaact tctttggcta aatgacgtgc
    26881 aattgttgcg gcagccggtg tttccaccat tacgccgatt tcaattgact cgtcaaacgc
    26941 tttaccttcg tcgcgcagtt cctgtttgta gatttcgatc tctttgcgca gtgcacgcac
    27001 ttcttcaaca gagatgatca tcgggaacat aatgcgcaat ttaccgaaag ccgaggcacg
    27061 caggatagcg cggagctgat cgcgcaggat ctctttacga tccatcgcga tacggatagc
    27121 gcgccagccg aggaacgggt tctcttcttt cgggaagttc atgtatggca gctctttgtc
    27181 gccgccgatg tccatggtac gaacgataac cgcctgcgag ccacacgctt cagccactgc
    27241 tttgtaagca gcaaactgtt cttcttcagt gggcagcgcg tcgcggtcca tgaacaggaa
    27301 ctcagtacga tacagaccaa cgccttcagc gccgttacgc tctgcacctt caacgtcacg
    27361 aaccgtacca atgttagcgc acacttctac ctggtgaccg tccagcgtaa tagctggcag
    27421 atctttcagt ttagcaagct ctgctttttc agaagccact tgctcctgaa cagcgcgcat
    27481 tttatcaata acttcgttgg ttggattgac gtaaacctga ttatttacgg catccagaat
    27541 cagatagtcg tcatttttca cctgagaggt gacgctaccg gtacccacga tagcaggcag
    27601 ttccagagaa cgcgccatga tagaggtgtg ggaagtacgg ccacccgcgt cggtgatgaa
    27661 acccagcacc ttcttcaggt tcagctgtgc ggtttcggac ggcgtcaggt cagcggcaac
    27721 cagaatgact tcatcctgaa tggcgctcag gtcgataatc ttcaggccca ggatgttgcg
    27781 cagcaggcgc ttaccgatat cacgtacgtc agccgcacgt tctttcaggt attcatcatc
    27841 cagctcttcc agggcagaag cctgaccttc gataacttca tgagcagctg cgtcagctgt
    27901 catgtgctta tctttaatca gggctatgat ttcctgctcc agctcctcat cttcgagcag
    27961 cataatatgc ccttcaaaga tggcttcttt ttcttcaccg aacgtttcac cagctttcgt
    28021 tttgatcgtt tccagctggg ctgatgcctt ggcacgaccg ctcagaaaac gttcaacttc
    28081 ctgatcaacc tggtcggcag aaattttttt ccggtcaatg acaatttcgt cttctttcag
    28141 aagcagagct ttaccgaaag cgatacccgg ggatgctaaa atgcctgaaa tcataaccct
    28201 accttacttg tgactgattt ttaaaagaac ccgggaaatt actcgagttc cgccatcagt
    28261 ttaaccagat gttcaaccgc tttctgctcg tcttcgcctt ctgcggagat agtcacaacg
    28321 gtaccttgag tcaggcccag agtctgcagt ttaaacaggc ttttcgcgct ggcgcttttg
    28381 ccgttggaag tcacagtaat ttcagaagtg aagcccttag cttcttttac aaactgggca
    28441 gcagggcggg tgtgcagacc gttcggagcg gtaatggtaa cttcttgctg gaacattgta
    28501 tttccccaac ttataggttt agtgttgtgg aactaaagtc tagcctggcg gttcgacttt
    28561 agcctgtatt gttagcgcca gtttttaaca gacgcgacgc acgaagagcg caatacagtc
    28621 cattgctggc gggtgttgac cgctgacgtt tcgtacgtca ttaaacatta tgccgctaaa
    28681 ggaagaattg aaccaaatca taaaatcgat tcagctttgg cttttcctgt aacgattaat
    28741 ttcgcgcatc aaaataatta gtctggttaa ataccagttc cgacaggctg aatcaatgcc
    28801 aggaggggcg aaagtttgaa gcaggccaca aaaaagcacc taaaaaggtg cttttttacg
    28861 catttttaac aagctggcat tactgttgca gttctttctc agtgaagaga tcggcaaaca
    28921 atgcggtgct taaataacgc tcacccgatg acggtagaat aaccacaata ttcttgttgg
    28981 taaagctttc atcttcttgt agtttcaacg ccgcagcaac agctgctcca gaagagatac
    29041 ctgcaagaat accttcttct tccatcagac gacgtgcggt agaaatcgct tcttcattgg
    29101 tgatgccaat gactttatcg accagcttga gatcgaggtt agctgggata aaaccagcgc
    29161 caataccctg aattttatgc gggccaggtt taatctcttc accagccagt gcctgggcga
    29221 taactggaga atcggttggc tcaacggcga cagagataag atcggtcttg cctttggtgc
    29281 ctttaatgta gcggctgacg ccagtcagcg taccgccagt cccaacgcca gcaataaata
    29341 catcaacctg accgtcggta tcttcccaga tctccggacc ggtggttttt tcgtgaattt
    29401 cagggtttgc cggattgctg aattgttgca gcagcaggta tttctctgga ttgctggcga
    29461 caatttcttc tgctttttgg attgcgcctt tcatgccttg agcaccttcc gtcagcacca
    29521 ggtttgcacc taacgctttc agcagcttgc ggcgttcaat actcatggtt tctggcatgg
    29581 tcagggtgag tttgtaaccg cgagcggcag ctacataggc cagtgcaatc ccggtattac
    29641 cgctggtcgg ttcaaccagt tcaacgcctg gtttcagcac gccgcgcttt tcggcatccc
    29701 aaatcatgtt ggcaccgata cggcacttaa cgctgaagct ggggttacga gattccacct
    29761 tcgccagaat gcgtccgtta ccgatgcgat tcaggcgaac cagcggcgtg tgaccgatag
    29821 tcagcgagtt atcttcaaaa atcttactca tggcctgtcc ttaactgtat gaaattggga
    29881 tacaacaggt agcatacccg ctcagagaat atgcggaagt aaggatttag catatctata
    29941 tacagaaggg aaataatgac atgcaagatg gaataagggg cggcataagc cacccctgtt
    30001 tcacacaaac ggtttacaaa ataaccggta gattgttacc gccacatcgc gtgtttatcg
    30061 cgatagcaat cgacccacat cgccgtcgcg ccacaaacgg caacgggcat gatgaacaga
    30121 ttaagcagcg ggatcatcgt aaacaggctg gttaaagcac caaactgcat attggtgatt
    30181 ttgcgtgtgc gcagggcggt gcgcatctct ttaaacggca ctttgtggtt atcgaagggg
    30241 taatcgcaat actggatggc taacatccag gcgctaaaca ggaaccacag taccggcgcg
    30301 acggtttgcc caataccagg gattaagtaa agaattagca atacaattgc gcgcggcaga
    30361 taccaggcaa atttttgcca ttcgcgtttc atgattcgcg gcacatcttt catgataccg
    30421 aaaatcccgg tatctggcgg tgtagcgcca gtcaatcgtg cttccagttg ttcagccaat
    30481 aaaccgttaa acggagcggc aatccagtta gcaatcgtgg agaagaaata gccaaacact
    30541 aacagcacag agatgacagc cagaggccac aacagataac tcagccattg tagccagtcc
    30601 ggaacgtaac tcatgagagt cgggatccag acatcgagct gtgtaaagag ccaccagaat
    30661 gcgcccccca tcaacaaaat attgaccagc agcggtaaaa taacgaaacg ccgaatccca
    30721 ggttgcgaga cgagcttcca gccttgcgca aaatagtaaa aaccgctgcg tggggcagat
    30781 gtgaatgatg aaaccataat caggatgagc tccttttgac caatcccagg aaaattctgc
    30841 gtattttacc gggtaattgc gcaatggaca gttaggatat gttcgaaaaa acagcaaaaa
    30901 gcacgatttc atctatcttt gtgctgtgaa agttaatagt gcacttgcac ttgaggtaat
    30961 cggcaaatac tcttagtgag taaatgtttg ccgtggtggc aaggtgttag aacaacagag
    31021 aatataatga tgcaggattt gcgtctgata ttaatcattg ttggcgcgat cgccataatc
    31081 gctttactgg tacatggttt ctggaccagc cgtaaagaac gatcttctat gttccgcgat
    31141 cggccattaa aacgaatgaa gtcaaaacgt gacgacgatt cttatgacga ggatgtcgaa
    31201 gatgatgagg gcgttggtga ggttcgtgtt caccgcgtga atcatgcccc ggctaacgct
    31261 caggagcatg aggctgctcg tccgtcgccg caacaccagt accaaccgcc ttatgcgtct
    31321 gcgcagccgc gtcaaccggt ccagcagccg cctgaagcgc aggtaccgcc gcaacatgct
    31381 ccgcgtccag cgcagccggt gcagcagcct gcctatcagc cgcagcctga acagccgttg
    31441 cagcagccag tttcgccaca ggtcgcgcca gcgccgcagc ctgtgcattc agcaccgcaa
    31501 ccggcacaac aggctttcca gcctgcagaa cccgtagcgg caccacagcc tgagcctgta
    31561 gcggaaccgg ctccagttat ggataaaccg aagcgcaaag aagcggtgat tatcatgaac
    31621 gtcgcggcgc atcacggtag cgagctaaac ggtgaactgc ttcttaacag cattcaacaa
    31681 gcgggcttca tttttggcga tatgaatatt taccatcgtc atcttagccc ggatggtagc
    31741 ggcccggcgt tattcagcct ggcgaatatg gtgaaaccgg gaacctttga tcctgaaatg
    31801 aaggatttca ctactccggg tgtcaccatc tttatgcagg taccgtctta cggtgacgag
    31861 ctgcagaact tcaagctgat gctgcaatct gcgcagcata ttgccgatga agtgggcggt
    31921 gtcgtgcttg acgatcagcg ccgtatgatg actccgcaga aattgcgcga gtaccaggac
    31981 atcatccgcg aagtcaaaga cgccaacgcc tgatacactt aaggcaaatt aactcctctt
    32041 cgaacccccg cttgtcgggg gtttttagca ttgatggtgc gatatggaat caatcgaaca
    32101 acaactgaca gaactgcgaa cgacgcttcg ccatcatgaa tatctttatc atgtgatgga
    32161 tgcgccggaa attcccgacg ctgaatacga caggctgatg cgcgaactgc gcgagctgga
    32221 aaccaaacat ccagaactga ttacgcctga ttcgcctacc caacgtgtag gcgctgcgcc
    32281 gctggcggct ttcagccaga tacgccatga agtaccaatg ctgtcactgg ataacgtttt
    32341 tgatgaagaa agctttcttg ctttcaacaa acgtgtgcag gaccgtctga aaaacaacga
    32401 gaaagtcacc tggtgctgtg agctgaagct ggatggcctt gccgtcagta ttctgtatga
    32461 aaatggcgtt ttagtcagtg ccgcgacccg tggcgatggc accaccgggg aagatatcac
    32521 gtctaatgtg cgtactattc gcgctattcc gctgaagctg cacggagaga atatcccggc
    32581 gcgtctggaa gtgcgtggtg aagtgttcct gccgcaggcg gggttcgaaa agattaacga
    32641 agatgcgcga cgcacgggcg ggaaagtttt tgctaaccca cgtaatgcgg cagctggttc
    32701 actgcgtcag cttgatccgc gtattacagc gaagcgaccg ctcacttttt tctgctatgg
    32761 cgttggtgtt ctggaaggtg gcgagctgcc ggatactcat cttggccgtt tactgcaatt
    32821 taaaaagtgg gggttgccgg tcagcgatcg ggtaacgctt tgtgaatcgg cggaagaagt
    32881 gctggcgttc tatcacaaag tggaagaaga ccgcccgacg ctgggctttg atatcgacgg
    32941 cgtggtgatt aaggtcaact cactggaaca gcaggagcag cttggctttg tcgcgcgtgc
    33001 cccgcgctgg gcggtagcgt ttaaattccc ggcgcaggag cagatgacct ttgtgcgtga
    33061 cgtcgagttt caggttgggc gtactggcgc gattacgcct gttgcgcgtc tggaacctgt
    33121 ccatgttgct ggcgtgctgg tgagtaacgc aaccttacac aatgcggatg aaatcgaacg
    33181 tcttggttta cgcattggcg ataaagtggt gattcgccgc gctggcgacg tgatcccgca
    33241 ggtggttaac gtcgtgcttt ctgaacgccc ggaagatacc cgtgaggttg tattcccgac
    33301 gcattgtccg gtatgtggtt ctgacgttga gcgtgtggaa ggtgaagcgg ttgcccgctg
    33361 taccggtggc ctgatttgcg gagcgcagcg taaagagtcg ctgaaacact ttgtttcccg
    33421 ccgtgcgatg gatgttgacg gaatgggcga caaaatcatc gatcagctgg ttgaaaaaga
    33481 atatgtccat actccggcag atctgttcaa actcaccgca ggcaaactga ccggactgga
    33541 gcgtatgggg ccaaaatcgg cacaaaacgt ggttaacgcg ctagaaaaag cgaaagaaac
    33601 cacctttgct cgcttcctct atgcacttgg catccgtgaa gtcggcgagg ccaccgcagc
    33661 aggtctggcg gcatatttcg gcacgctgga agcgctggaa gccgcttcga ttgaagagct
    33721 gcaaaaggtg cctgatgttg gcattgtcgt tgcatcccac gttcacaact tctttgccga
    33781 agaaagcaac cgcaatgtca tcagcgagct gttggcggaa ggtgttcact ggcctgcgcc
    33841 gatcgttatc aacgcggaag agattgacag cccgtttgct ggtaaaaccg tggtgcttac
    33901 gggcagctta agtcagatgt cacgtgatga cgctaaagct cgactggtcg aactgggcgc
    33961 gaaagtcgcg ggcagcgtgt cgaagaaaac cgatctggtg atagcgggtg aagctgcagg
    34021 atctaaactg gcgaaggcgc aggaactggg cattgaagtc atcgacgaag cggaaatgct
    34081 gcgtttgctg ggtagctgag atggaaaaag agcagctgat tgaaatagcc aatacgataa
    34141 tgccgtttgg caaatacaaa gggcgtcgct taatcgacct gccggaggag tatctgctgt
    34201 ggtttgcccg caaagatgag ttcccggcag ggaagctcgg tgagctaatg caaatcacgc
    34261 tgctgattaa aaccgagggg ctgacgcaac tggtccagcc cctgaagcgt ccgctttaag
    34321 ctttatcggt gctgctttcc tgctgcgcct gtaactgttc ggtctggcgt ttgtatcgac
    34381 gcgccagcac cgcacagacc atcaattgga tctgatggaa aatcatcagg ggcagcacca
    34441 tcataccgat caccgatgtg gggaacagaa tgtttgccat cgggatgcca tttgccagac
    34501 tctttttcga accacaaaag acgatagtaa tttcatctgc cttattgaag cccagtcggc
    34561 gtgccataaa gacgttaact acaatcacga tagccagaag aacgcagctg accaccacga
    34621 taaacagcaa tgatccccag ccaactttat gccagatacc attaacgacg gcttcgctga
    34681 acgctgtata aaccaccaac agaatggacg tctggtcagt tttcgcaatc cattttttat
    34741 tgcgcgacac ccagtcacca atccacggcc gggaaagatg ccccaacaca aacggcagca
    34801 gcagttgcag cataatttta ccgacctgct caaggctgcc ccctgcaccg tgaacattca
    34861 tcaccagacc aaccagcaat ggtgaaagga aaatccccag caggctggat gctgacgcag
    34921 aacaaaccgc cgccgcgacg ttaccgcccg ccattgacgt gaaggcgatt gcagactgca
    34981 cggtagccgg gagaatgcac aagtagagaa aaccggagta gagcatcggg tcgacattta
    35041 ccggtttcca ccaggcaaac agtacaccca gaatcggaaa cagcacgaag gtgctgcaaa
    35101 ttacccacaa atgcagtcgc cagtgaccac cgccagcaat aatcgcctca cgcgacaact
    35161 tcgcgccgtg cataaagaac agcagggcaa tcgctgcggt ggtcagattt tcaaagaagg
    35221 ggacgaaatc gcctctggcc ggaaagaaag aggccagcaa caccaccgtg atcagggtta
    35281 aggtgaaagg atcgaggata cgaaaaagtt tcataaagac tccagatgat cgatggttct
    35341 attgtgcttc ttccagtttg agaaataaaa ttgatttatt gcatccattc atgaagaaaa
    35401 cagatgaatt attctttaaa acaattaaag gttttcgtca cagtagcgca ggagaaaagt
    35461 tttagtcgtg caggagagcg tatcggcctg agccagtcgg cagtgagtca cagtgtgaag
    35521 gaactggaaa atcatactgg tgttcgcctg ctggacagaa ccacgcgtga agtggtgctt
    35581 acagatgcag ggcagcagct ggctttgcgt cttgagcgac tgctggatga actgaacagc
    35641 acgttgcgcg ataccgggcg tatggggcaa caactgagcg gaaaagttcg ggtcgctgcc
    35701 agccagacca tttccgcgca tcttattccg caatgcattg ctgaaagcca tcgccgctat
    35761 ccagatattc agtttgtcct gcacgatcgt ccgcagcagt gggtgatgga aagtattcgc
    35821 cagggagatg tcgactttgg catcgtcatc gatccaggcc cagtgggcga tctgcaatgt
    35881 gaagcgattc tttccgagcc tttctttctg ctttgccatc gcgatagcgc tttggccgtc
    35941 gaagattatg taccctggca ggcattacag ggagcgaaat tggtgttgca ggattacgcg
    36001 tcaggcagcc gaccgctgat tgacgcagcg ctggcgcgca atggtattca ggcgaatatt
    36061 gtgcaggaga ttggacatcc ggcgacgctg ttcccgatgg tagccgcagg catcggcatc
    36121 agtattcttc ccgcactggc gctaccattg cctgaaggta gcccactggt ggtaaagcgc
    36181 attacgccgg ttgttgaacg ccagttgatg ctggtgcgca ggaagaaccg ttcactttcc
    36241 accgccgctg aagcgttatg ggacgtggta cgagaccagg gcaatgcgct aatggctggt
    36301 cgggaagggg atccgttgta tcagatatag agtgaattca tactgccggg agccccggca
    36361 gtaatcaatc agaagttaaa tttgatggag tagattatcg catcctggta aaaatcttcg
    36421 cccagtttgt catccgcata tcgatactga atccccgtag taatgtgtga tgttgcattc
    36481 caccacagtg caactgcgcc attcagccct tctttaccgc cattacccgc tgcataggta
    36541 gcgtcacggt caaactcgta ctcgttccag ttggtaagag tgaatttctc gctgcccagc
    36601 ataaagttgt aacctgcaac ccagccggcg acatagccgt tgtcgccagt gtaataggtt
    36661 tgatctgtat aacgcttagc aaagaacggt ttgaaccatc aaccgctgcc ggtgaaattg
    36721 tagccgatac cgtacaggaa catatcgtcg tggaaattca cgcgattcgc agaaccgtag
    36781 gtgccatacg catgcaagta gagattaaat ccggtgtcac ccaggtaaat acggttggta
    36841 tttttaaagg tataacgctg ctcgctacct ggtttattat ggcgaccgtt atagaagttt
    36901 tcccagtcga agaacccgta catttctccc caactaaagt tagcgccacc ttcaaattca
    36961 agataaccaa aatcatcttt gtgtgatttg gtcgacgatt tttcggtagt ccggctggtc
    37021 cagtccagat aatgcacgcc gatatctgca aaaccgcctt taaattctgc atgagataca
    37081 acaggtatcg ctaatataga ggaaagtgtc agagttaaaa gatgtttttt catagcataa
    37141 ttccctatgc cgatcgttat tagtagattg cttaataaag tggtttgcta aaaacgacat
    37201 attgttaatg caaaggaatt aatatcgcca atacagtttt taccgtggat taatagagaa
    37261 aagatataaa tccatcgata tcgaatcgct attgaatgcg tgagaaaagt gtattggtaa
    37321 gagccgggac aacctcgcta taaaaaggcg ttagattcca ccctacagaa aaaggatatg
    37381 tatgtctcag gttcaatttt ctcataaccc gctgttttgc atagatatta tcaagactta
    37441 taaacctgat ttcacgccac gagtggcctt tattttaggt tccgggctcg gcgcgctggc
    37501 cgatcagatt gagaacgctg tcgcaatttc ctacgaaaag ctgccgggct tcccggtaag
    37561 tacggtacat ggtcatgcgg gggagttagt gctgggccat ctccagggcg taccggtggt
    37621 atgtatgaaa ggtcgcggac atttctacga aggtcgtgga atgaccatta tgactgacgc
    37681 aattcgtacc tttaagctgc tgggctgcga gctactgttc tgcaccaatg cggcaggctc
    37741 gctgcgtccg gaagtggggg caggcagcct ggtcgcattg aaagatcata tcaacaccat
    37801 gcccggtacg ccgatggtgg gtcttaacga tgatcgtttt ggagagcgct tcttctcact
    37861 ggcaaatgcc tacgatgcgg aataccgcgc actgttacaa aaagtggcga aagaagaggg
    37921 tttccctctg acggagggcg tgttcgtctc gtatccgggg ccgaatttcg agactgcggc
    37981 ggaaattcgc atgatgcaaa ttattggtgg ggatgttgtt ggtatgtctg tggtgcctga
    38041 ggttatttca gctcgccatt gcgaccttaa agtcgttgcg gtctctgcga ttaccaatat
    38101 ggcggaaggt ttaagcgatg tgaaattgtc acatgcgcaa acgctggcgg ctgcggagct
    38161 ctctaagcag aacttcatca accttatttg tggctttctg cgcaaaattg cctgagaaaa
    38221 acaataagaa acggcctcgc gggtgaggcc aaatgatgcg acaaggaaac gattatgagc
    38281 atcgcgatgc gcttaaaggt aatgtccttt ttgcaatatt ttatctgggg gagctggctg
    38341 gttaccctcg gctcttacat gattaatacc cttcatttca ccggcgctaa tgttggcatg
    38401 gtttacagtt ccaaagggat cgccgcgatt attatgcctg gtataatggg gatcatcgca
    38461 gacaaatggc tgcgcgcaga acgtgcatac atgctgtgtc acctggtgtg tgcgggcgta
    38521 cttttttatg cggcatccgt aactgatccg gatatgatgt tttgggtgat gttagtcaat
    38581 gcgatggcgt ttatgccgac tattgcgtta tcgaacagcg tctcttattc ctgtcttgcc
    38641 caggcagggc ttgacccggt gaccgctttc ccgcccattc gcgtttttgg tacggtgggg
    38701 ttcattgtcg cgatgtgggc agtaagcctg ctgcatctgg aattgagtag tctgcagctg
    38761 tatatcgcgt ccggtgcgtc attgctgctg tcggcttatg cgctgacttt gccgaagatt
    38821 ccggttgcgg agaaaaaagc gaccacatcg cttgccagca agctgggtct ggatgccttc
    38881 gtgctgttta aaaatccacg catggccatc tttttcctct ttgccatgat gctgggtgcg
    38941 gtactgcaaa ttaccaacgt ttttggtaat ccgttcctac atgatttcgc ccgtaactcg
    39001 gagtttgctg acagttttgt ggtgaaatat ccctccattt tactgtcagt ttcacagatg
    39061 gcagaagtgg gctttatact gactatccca ttctttttaa agcgatttgg cattaaaacc
    39121 gtcatgctga tgagtatggt ggcctggacg ctgcgctttg gcttcttcgc ctatggcgat
    39181 ccgtcaacaa ccggatttat tttgctgctg ctgtcgatga ttgtttatgg ctgtgcattc
    39241 gatttcttca atatttctgg ttcggtattt gtcgaacagg aagttgattc cagcattcgt
    39301 gccagcgcgc aggggctctt tatgaccatg gtaaatggtg tcggcgcatg ggttggctcg
    39361 attctgagtg gcatggcagt agattacttt tcggtggatg gcgtaaaaga ctggcaaact
    39421 atctggctgg tgtttgcagg atatgctctt tttctcgcag tgatattttt ctttgggttt
    39481 aaatataatc atgaccctga aaagataaag catcgagcgg tggctcatta aaaggattcg
    39541 cagctctgct cttcagagct gcttttatga taaaggttaa ttagtgaaag atattttatt
    39601 ttcttccggc gttggttttg gcattggtgc tttgtttaca atcgtgcgct tgcctattcc
    39661 tgtaccaaat gtattaccag gtatattatc cattgtgttt atgtatgtcg gatatctggt
    39721 ggtgaaatac tttatgcaat gataatttaa tacgatgtat ttattatatg gagcacttaa
    39781 ttatggaacg cgtatacaga acagatctta agttgctccg ttattttctt gccgtagcgg
    39841 aagagttgca ttttggccgc gcagcagcgc gtttaaatat gtctcagcct ccgctcagta
    39901 ttcatattaa agagctggaa aatcaactcg gcacgcagct ttttattcgc cattcgcgca
    39961 gcgtcgtact gacacacgcg ggcaaaatct tgatggaaga atcgcgtcga ttgctggtga
    40021 atgctaataa tgtattggct cgggttgaac aaataggtcg gggagaagca gggcggattg
    40081 aactcggcgt agtgggaacg gctatgtggg gacggatgcg cccggttatg cggcgattcc
    40141 tcagggaaaa tcctaacgtt gaagttcttt ttcgcgaaaa gatgcccgcg atgcaaatgg
    40201 ccctgctgga acgacgtgaa ctcgatgccg ggatctggcg aatggcgaca gaaccaccga
    40261 caggttttac cagcttacgg ttgcatgaat cggcgtttct ggtggcgatg cctgaagagc
    40321 atcatctctc atcattttcc accgttccgc tggaagcgct acgtgacgag tattttgtta
    40381 caatgccgcc cgtgtacact gactgggatt ttttgcagcg agtttgccag caggtgggat
    40441 tttcaccggt tgttattcgc gaagttaatg aaccgcaaac ggtactcgcc atggtcagta
    40501 tgggtattgg tatcacattg atagcggaca gctacgcaca aatgaactgg ccaggtgtca
    40561 ttttccgtcc gctcaaacag cgcatccctg cagatttata tattgtttat gaaacacagc
    40621 aggtaacgcc cgcgatggtt aagctgttgg ctgcattgac acagtaggtg aggagacagg
    40681 aattgccgga aatagtcgtc tgctaagtga agcgcatcag gcaatttcgc aaatcgca
//
LOCUS       DAHBHC010000043        40652 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHBHC010000043 DAHBHC010000000
VERSION     DAHBHC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.043"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(119..361)
                     /locus_tag="M2A35_002669"
     CDS             complement(119..361)
                     /locus_tag="M2A35_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7198953.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(580..798)
                     /locus_tag="M2A35_002670"
     CDS             complement(580..798)
                     /locus_tag="M2A35_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCB7198954.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1384..1674)
                     /locus_tag="M2A35_002671"
     CDS             complement(1384..1674)
                     /locus_tag="M2A35_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7198955.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1675..1986)
                     /locus_tag="M2A35_002672"
     CDS             complement(1675..1986)
                     /locus_tag="M2A35_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7198956.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2215..3123
                     /locus_tag="M2A35_002673"
     CDS             2215..3123
                     /locus_tag="M2A35_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7198957.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="M2A35_002674"
                     /gene_synonym="yiiD"
     CDS             complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="M2A35_002674"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCB7198958.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAIADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="M2A35_002675"
     CDS             complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="M2A35_002675"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCB7198959.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQTGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4607..5479)
                     /locus_tag="M2A35_002676"
     CDS             complement(4607..5479)
                     /locus_tag="M2A35_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCB7198960.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="M2A35_002677"
     CDS             complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="M2A35_002677"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCB7198961.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6171..6956)
                     /locus_tag="M2A35_002678"
     CDS             complement(6171..6956)
                     /locus_tag="M2A35_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCB7198962.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6990..7886)
                     /gene="yihV"
                     /locus_tag="M2A35_002679"
     CDS             complement(6990..7886)
                     /gene="yihV"
                     /locus_tag="M2A35_002679"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCB7198963.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            8054..8950
                     /gene="yihU"
                     /locus_tag="M2A35_002680"
     CDS             8054..8950
                     /gene="yihU"
                     /locus_tag="M2A35_002680"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCB7198964.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8974..9852
                     /gene="yihT"
                     /locus_tag="M2A35_002681"
     CDS             8974..9852
                     /gene="yihT"
                     /locus_tag="M2A35_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCB7198965.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9869..11110
                     /gene="yihS"
                     /locus_tag="M2A35_002682"
     CDS             9869..11110
                     /gene="yihS"
                     /locus_tag="M2A35_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCB7198966.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            11248..12150
                     /locus_tag="M2A35_002683"
     CDS             11248..12150
                     /locus_tag="M2A35_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCB7198967.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            12349..14385
                     /gene="yihQ"
                     /locus_tag="M2A35_002684"
     CDS             12349..14385
                     /gene="yihQ"
                     /locus_tag="M2A35_002684"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCB7198968.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILIAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14431..15816
                     /locus_tag="M2A35_002685"
     CDS             14431..15816
                     /locus_tag="M2A35_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7198969.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RLGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15859..17262
                     /locus_tag="M2A35_002686"
     CDS             15859..17262
                     /locus_tag="M2A35_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7198970.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17330..18022
                     /gene="ompL"
                     /locus_tag="M2A35_002687"
     CDS             17330..18022
                     /gene="ompL"
                     /locus_tag="M2A35_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCB7198971.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENRDAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18113..19378)
                     /locus_tag="M2A35_002688"
     CDS             complement(18113..19378)
                     /locus_tag="M2A35_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7198972.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19480..20460)
                     /locus_tag="M2A35_002689"
     CDS             complement(19480..20460)
                     /locus_tag="M2A35_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCB7198973.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQDDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20468..21178)
                     /locus_tag="M2A35_002690"
     CDS             complement(20468..21178)
                     /locus_tag="M2A35_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCB7198974.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21395..23218)
                     /gene="typA"
                     /locus_tag="M2A35_002691"
     CDS             complement(21395..23218)
                     /gene="typA"
                     /locus_tag="M2A35_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCB7198975.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23591..25000
                     /gene="glnA"
                     /locus_tag="M2A35_002692"
     CDS             23591..25000
                     /gene="glnA"
                     /locus_tag="M2A35_002692"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCB7198976.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25174..26223
                     /gene="glnL"
                     /locus_tag="M2A35_002693"
     CDS             25174..26223
                     /gene="glnL"
                     /locus_tag="M2A35_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCB7198977.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26235..27644
                     /gene="glnG"
                     /locus_tag="M2A35_002694"
     CDS             26235..27644
                     /gene="glnG"
                     /locus_tag="M2A35_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCB7198978.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27756..27866
                     /locus_tag="M2A35_002695"
     CDS             27756..27866
                     /locus_tag="M2A35_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCB7198979.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(28056..29429)
                     /gene="hemN"
                     /locus_tag="M2A35_002696"
     CDS             complement(28056..29429)
                     /gene="hemN"
                     /locus_tag="M2A35_002696"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCB7198980.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29618..30127)
                     /gene="yihI"
                     /locus_tag="M2A35_002697"
     CDS             complement(29618..30127)
                     /gene="yihI"
                     /locus_tag="M2A35_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCB7198981.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            30709..31341
                     /locus_tag="M2A35_002698"
     CDS             30709..31341
                     /locus_tag="M2A35_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCB7198982.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31723..34509)
                     /gene="polA"
                     /locus_tag="M2A35_002699"
     CDS             complement(31723..34509)
                     /gene="polA"
                     /locus_tag="M2A35_002699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCB7198983.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            34873..35805
                     /locus_tag="M2A35_002700"
     CDS             34873..35805
                     /locus_tag="M2A35_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCB7198984.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(35846..37276)
                     /locus_tag="M2A35_002701"
     CDS             complement(35846..37276)
                     /locus_tag="M2A35_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCB7198985.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(37431..38057)
                     /gene="dsbA"
                     /locus_tag="M2A35_002702"
     CDS             complement(37431..38057)
                     /gene="dsbA"
                     /locus_tag="M2A35_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCB7198986.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(38074..39060)
                     /gene="srkA"
                     /locus_tag="M2A35_002703"
     CDS             complement(38074..39060)
                     /gene="srkA"
                     /locus_tag="M2A35_002703"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCB7198987.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(39137..39406)
                     /locus_tag="M2A35_002704"
     CDS             complement(39137..39406)
                     /locus_tag="M2A35_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCB7198988.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            39476..40060
                     /gene="mobA"
                     /locus_tag="M2A35_002705"
     CDS             39476..40060
                     /gene="mobA"
                     /locus_tag="M2A35_002705"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCB7198989.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHGGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            40042..40569
                     /gene="mobB"
                     /locus_tag="M2A35_002706"
     CDS             40042..40569
                     /gene="mobB"
                     /locus_tag="M2A35_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCB7198990.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT        10056 a        10317 c         9786 g        10493 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc ctgataaggc gctcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaatcg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg aggcgttttc cagatacgat
      481 gtaacagttg cagttattat aagttcaagt aaaacagcct tgtattaata ccagaataag
      541 ggcatctgtt tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca
      601 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
      661 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      721 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      781 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      841 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
      901 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      961 tagttatcgc ccaggatgaa ttatgggtgc tatgaatata ttatattcag agatatttat
     1021 ttgcataatc tgaatggtgt agccgaaaaa cgtcagcatc ctaacagcac caggatttaa
     1081 ggtgaaatta atcatccatc acagaccggt cagtactgcc accaaccggt ctttacttgt
     1141 tcatcgttat aattttgttg gtaacagttc gggaaacaag gaattccttc cggaaccccc
     1201 cggtatcatc cgatagcatt gacgtcaatc atcagcgcga cgatgagttt ttttaatcgc
     1261 atcatagggt ggcgacgttc agcacgatta cgggtcatag caaaactcct gggcaggcga
     1321 ccactatgcc gctttcacag gagttctgaa tgctagtgct ttctgtatgg cataaccatg
     1381 cctctaacgg ttaagtgctt gcagcacatg ttgcggatca ttggcaatcg ctcgcagcaa
     1441 agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc cagttacgca acgtgtctac
     1501 gcttacagaa ataagatcgg caaatttaga ttgtgacaac cctgaagcgc ggcgtatttc
     1561 tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc tgtcgcgttc cttcaataat
     1621 ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat ccatttacca
     1681 cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat ctttaatacc
     1741 cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa agtaaataac
     1801 cctgacacca gcacgtttcc cctttcctcc tgaaacccat cgaacctttc ttaacccacc
     1861 agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact gaaaacgact
     1921 gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg tttcgatgaa
     1981 gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc attgacgtac
     2041 ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg ccgggtttgt
     2101 ctttagattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc tgtatccatc
     2161 cagcaaaaac gggtgtataa gactgtacat tcatacagca aaataagttc tactatggca
     2221 ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg ctgcgggtcg cccctcatcg
     2281 cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt ccttgcgggt
     2341 gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgac cttacgaata
     2401 gtgtcgccgc tgaatgcccc gacattactc ccgacgataa tttattacta cggtggctgt
     2461 tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc ctattacggg
     2521 caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg aacatacttt ccccgccgcc
     2581 catgatgatg cgcaacgagg tgcagaaata gtctggcaac acgccgaacg actcggcgtt
     2641 gataaacaac gtataactct tgccggtgac agtgcgggcg gacatctggc gctggtaact
     2701 gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt aatttatcct
     2761 atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga ttacatcatc
     2821 acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga acgcaggcat
     2881 cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt acatatcatt
     2941 accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg catgacaggg
     3001 caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt ttttcagtta
     3061 ggtggtatca gcgaggccgc gcgatcggca ctacgggatg ttgcctggag agcgggaagg
     3121 taacttcgga cctgggccct gaaaaggttt tcagattgaa aaccttttca gtcaagtaaa
     3181 acgcccctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
     3241 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcaacttg
     3301 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
     3361 aaggtcggct atcgcatgag gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc
     3421 cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga taagccccca
     3481 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
     3541 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatgcgcac
     3601 gcccattttt tcgcttaatg gaatgtgttc ataccacgcc tgttgcagct gggcgcacca
     3661 gtcaccgcga tgcagaatat catccagagt ggcaacgggt ttaatcatca aaaaatggcg
     3721 aatcggcgtg gtagttgggg tggtgatctc cccctgatta atgaatccca gcttggcgaa
     3781 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
     3841 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtcgggatg
     3901 aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacaatc ggcctaccgc
     3961 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
     4021 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
     4081 gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag cgatactctc
     4141 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
     4201 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
     4261 tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
     4321 gcgcctttgg agaaacttgg gcgcatccct cgttcggtat ctgcggcgag ggtaaactgg
     4381 gaaaccacca gtacactgcc gcccgtctgt tgcacgttga gattcatctt gccttcggca
     4441 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
     4501 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
     4561 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
     4621 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
     4681 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
     4741 ccgccagcac gccgtaaatg agctgatatg acgggaacat ggtgatataa agcgcgaaac
     4801 ctttctttcc tgcttcgaac aggagtgcag cgacaaacgc gccgacaatc gcgtcgcggt
     4861 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
     4921 gcagcggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc gcccagcgca
     4981 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
     5041 aaatcatcca gtacacggca aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
     5101 tggtattcaa cgcgctatcg atggagtaca tcaacaataa tgcggtgacg atcagcccgc
     5161 acgcgccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata taccgctgaa
     5221 taacatcgcc agtagcaggc aaaaagttgg caaaaataaa gtgacgcaac tgaatgctga
     5281 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
     5341 atgagagcaa agacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
     5401 tacgttgcca gagtagtttt agccaggccc atagtggacg agtacggtgc ctggctttgt
     5461 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
     5521 caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg cgttatcgtc
     5581 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
     5641 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
     5701 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
     5761 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
     5821 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
     5881 ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct catgctgatg
     5941 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
     6001 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
     6061 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
     6121 gggtttggct atgtggcaag gtatgaagcc aggttaacgg tcacaaaaat ctacgcgtct
     6181 tcctggggaa gaatgatttc aatttggctg tcttgtaagc gtgattttag cgcagcgggc
     6241 ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt taccaatttc aaccgccgcc
     6301 gaagcatgat acttagtgtg atcggcgacc agcagaatat ttctcgcgtg cgccatcatc
     6361 gttttcacca cgttagcttc gtttacatca aactccatca acgcgccatc gctctcaatc
     6421 gccccaacgc ttgttaccag ataatcagca cgaaaatcag ccacaaagga cgccgctgaa
     6481 gggccaatga tcccgctatt atgagagcgc aacgtaccgc cgggcaccat cacttcaaag
     6541 cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt tggtgattat ccgcaaatga
     6601 ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg taccaatggt gataaatatt
     6661 gttgaaccat caggaatata gtctgccacc gcttcggcaa tcgctttttt ttcctcggtt
     6721 tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc tggaagcccg acccgcgcca
     6781 ccgtgatggc gcgtaatcag gccttgctcg cttaatttac gaatatcccg gcggaccgtc
     6841 tgcgtggaga catccagcag atttgccagc tcatcaatat tcatataccc acgctcggcg
     6901 atcagcatga ggagttggtc gtgccgcggg ttaccggtta gttcggtaag gctcatgaaa
     6961 attcctcgaa aaaccatcac cctggcattt tatacaaaaa gtgacaaaaa agatcgggtt
     7021 tgatcacagt cagggatccc ggctcgtcca ccgggacgtg tgcattttaa cgccgctaca
     7081 ccgctggcga agcggactga ctccgctaaa tccccacttg ttgccagcgc caccgccaaa
     7141 gcgccgtgaa aaacatcacc cgcacctgtg gtatctacca catcaacttt gaaggccggt
     7201 tgatgctgac gcccaccatt ttccagccag tcgcagcctg cgctaccctg ggtcacatag
     7261 acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac tggccatctc tttcacgccc
     7321 gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat cgcttaatgc caccagctca
     7381 ctgatatcct gcggcgtaat gtccccgtcc agaacggtca tcacacccgc ctgacgggcc
     7441 agggtgaagg cttttttagc gccgtcgtgc cagcgtacat ctgccagcac aacatcccac
     7501 tgagagaaat caatttcctc caaccactct gcgtcaggca gcagatccgg gctggggtag
     7561 ttaatgatta tccgctcgcc tttggtatcc accatgatgg cggattgcga agatttcgcc
     7621 tggttatacc gtttggtgta acgggtgtta acgccccagg attccagctc tgccagcagg
     7681 ctgttgcccg tgtcgtcatc acctacgcga ccaataaaat cgacctgcgc ccccagcctt
     7741 gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa cttccgtata atttctcgcc
     7801 acgtatttac cgctctccgt cggtaaccct tccacgtaat agatgcgatc catcacggtt
     7861 atacctacac aagcaacacg aatcatggtc attccttaaa cattttggga tgcgttcatt
     7921 ttaatttcac caattgttta aaaagtgacg gctgtcaatt ttttgacgat aaattacaaa
     7981 tacgatcaaa aacagacaag caaagcatgt cataaatgac aaaaagtgac atcactgtat
     8041 tcaggagagg gttatggcag caatcgcgtt tatcggttta ggacaaatgg gttcgccaat
     8101 ggcgagcaat ttattgcagc aagggcatca acttcgcgtc tttgatgtga acgccgaagc
     8161 tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc aacccggcgc aggccgctaa
     8221 agatgccgaa tttatcatta ccatgttgcc gaatggcgat ctggtgcgca acgtgttgtt
     8281 cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg ctggtcattg atatgtccac
     8341 catccatccg ctgcaaaccg ataaattgat tgccgatatg caagccaaag gcttcagcat
     8401 gatggatgtt ccggtaggcc gtacttctgc aaatgccatt accggtactc tgttactgct
     8461 ggctggcggc accgctgaac aagttgaacg tgccacaccg atcctgatgg cgatgggcag
     8521 tgagttgatc aactctggcg gtccgggcat ggggatccgc gttaagctca tcaacaacta
     8581 catgagcatc gcgctcaatg cgctttcggc agaagccgcc gttttgtgcg aagccctgaa
     8641 tcttcccttc gatgttgccg tcaaagtgat gagcggtacc gccgccggta aaggtcactt
     8701 caccacttcc tggccaaaca aagtcctcag cggagatctt tctcccgcct tcatgatcga
     8761 tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc aaccagctgc atgtgccaat
     8821 gccgctgggg gccgcctcac gggaggttta tagccaggcg cgcgcagcgg gtcgcggtcg
     8881 ccaggactgg tccgccattc tggaacaggt ccgtgtcagt gccgggatga ctgccaaagt
     8941 aaaaatgtaa cgactggata aaggaataaa tgaatgaata agtacaccat caacgacatt
     9001 acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc catgcgcatg
     9061 atgtttgccg cggctggggc acccgctccg gtagccgata gcgttttaac tgatttcaaa
     9121 gttaacgctg caaaggccct ctcgccttat gcctcggcga ttctggtaga tcaacaattc
     9181 tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa gttgcgccat gattgtcgcc
     9241 gccgatgagt tcattcctgg caacggtatt ccggtcgata gcgtggttat tgaccgcaaa
     9301 atcaatccgc tacagatcaa acaagacggt ggcaaagcct taaaactgct ggtgctgtgg
     9361 cgtagtgatg aagatgcgca gcaacgtctg gatatggtga aagagttcaa cgaactgtgc
     9421 cactcacacg gtctggtaag catcattgag ccagtcgtcc gcccaccgcg tcgtggcgat
     9481 aaattcgatc gcgaacaagc gatcatcgat gccgccaaag agctgggcga cagtggcgct
     9541 gacctctaca aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga gcttctctgt
     9601 gcttcacaac gtctgaatga ccatatcaat atgccatggg tgatcctctc ttccggtgtc
     9661 gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc atcgggattc
     9721 ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac cagacaacga gctgatgctg
     9781 cgtgacgttt gcgcaccgaa attacaacaa cttggcgata tcgtcgacga aatgatggct
     9841 aaacgccgct aagatgagga taaaaggaat gaaatggttt aacaccctaa gccacaaccg
     9901 ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg tagtgccgac
     9961 tggttttggc tggttaggca ataaagggca aatcaaagaa gagatgggca cccatctgtg
    10021 gatcaccgct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc gacctggcgc
    10081 ttactcgttg gttgatcacg gtatcaaagc catgaacggc gcactgcgtg ataaaaaata
    10141 tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca aacagggcta
    10201 tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc acccggaagc
    10261 gcgcaagctg ctcgattaca ccattgaaat tatcgagaaa tatttctgga gcgaagaaga
    10321 gcagatgtgc ctggaatcct gggacgaagc cttcagcaaa accgaagagt accgcggcgg
    10381 caatgccaat atgcacgcgg tggaagcttt cttgattgtt tatgacgtca ctcacgacaa
    10441 aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa
    10501 taatcattat cgcgttaacg aacacttcga tacccagtgg aatccgctgc cggattacaa
    10561 caaagataac ccggcgcatc gcttccgcgc gtttggaggt acaccaggcc actggatcga
    10621 atggggccgt ttaatgctgc acatccatgc tgccctggaa gcccgttgcg aacaaccacc
    10681 agcatggctg ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg atgcctgggc
    10741 acccgatggc gcggacggga ttgtttatac cgttgactgg gaaggaaaac cggtggtccg
    10801 cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg gcctacgcgc tctacaccgt
    10861 cactggcgat cgccagtatg aaacctggta tcaaacatgg tgggagtact gcattaagta
    10921 cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca ataaggtcac
    10981 caccaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc tggtgatccc
    11041 gcgtatcccg ttagcgcccg gcatggctcc agcggttgca gcgggtctgc tggatattaa
    11101 tgcgaaataa ctgatctgaa aatagggtgc gggttttccc ctcaccctaa ccctctcccc
    11161 aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg acatcagcct cacgggaggc
    11221 tgtatgtcgt taattaaggt gccttgtatg caaatcacca atatgcattg cagtggacag
    11281 accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg tgctggtctg
    11341 gctgaattaa cctttcaggg ttgtcattta gtcatcccgc acaaacccga agagatgccg
    11401 ctggcgcatc tgggtaaagt gcttattccg tggccgaacc ggatcgccaa cggctgttac
    11461 cgctatcagg ggcaggaata tcagttaccc attaatgaac atagttcaaa agccgctatt
    11521 cacgggctgc ttgcctggcg agactggcaa attagtgaat taaccgccac cagcgttacc
    11581 ctgacagctt ttctgccgcc cagttacggc tatccgttta tgctggcttc acaagttgtt
    11641 tattcgctca acgcacacac gggtctttcc gttgagatcg caagccagaa tatcggcact
    11701 gtcgcagcgc cttatggcgt gggtattcat acttatttaa cctgcaactt aacatctgtt
    11761 gatgaatatc tgttccagct tccggcaaac caggtttatg ccgttgatga acacgtaaat
    11821 ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc gaaaacaata
    11881 gccgcgacaa aaatcgacca cacctttaaa accgctaatg atctgtggga aataaccatt
    11941 acccatccac aacaggcgct ttcggttagc ctttgttgcg atcaaccctg ggtacaaatt
    12001 tatagcggtg aaaaattaca gcgtcaggga ctggcagttg aacctatgag ttgcccgcct
    12061 aacgccttta attcaggtat cgacttatta ttactggaac caggaaaacc gcaccgcctg
    12121 ttttttaaca tttacggaca gagaaaataa cttgtattga ttagcagtca aaatcattga
    12181 agaacatttc actcgctaaa ggcaacatta tgtatcttca atttattaag ttaaaaacag
    12241 ctcagcttct gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt
    12301 aataacagga aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca
    12361 cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac cctacatttt
    12421 caacaacgtc ttattttaac ccatagcaaa gataatcctt gtttatggat tggctcaggt
    12481 atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa
    12541 attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc
    12601 cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg
    12661 ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa
    12721 ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgtggcaaa
    12781 ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc
    12841 tggcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag
    12901 cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg ctatatgaac
    12961 ttcgacttta gtgccccgga ataccatgaa ctggcgctgt gggaagacaa agcaacgctg
    13021 cgttttgaat gtgctgacac atacatttcc ctgctggaaa aattaaccgc cctgctggga
    13081 cgccagccag aactgcccga ctggatttat gacggagtaa cgctcggcat tcagggcggg
    13141 acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggtcaacggc
    13201 atctgggcgc aggactggtc cggtattcgt atgacctctt ttggcaaacg cgtgatgtgg
    13261 aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggaat
    13321 caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc
    13381 tgcgaagaag cggcacaaca cggctatctg gcaaaagatg cctctggcgg tgactatctg
    13441 gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc
    13501 tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct
    13561 gacttcggcg agtatctgcc caccgacact tacctgcata acggcatcag cgccgaaatt
    13621 atgcataacg cctggcccgc gctgtgggcg aaatgtaact acgaagccct tgaagaaacg
    13681 ggcaagctcg gcgagatcct tttctttatg cgcgccggtt ctaccggtag ccagaaatac
    13741 tccaccatga tgtgggcggg cgaccagaac gtcgactgga gtctcgacga tggcctggcg
    13801 tcggttgtcc cggcggcgct gtcgctggca atgaccggac atggcctgca ccacagcgac
    13861 attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg
    13921 tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac
    13981 aactggcagt ttgacggcga cgcagaaact atcgcccatt ttgcccgtat gacctccgtc
    14041 ttcaccaccc tgaaacctta cctgaaagag gctgtcgcgc tgaatgcgaa gtccggcctg
    14101 ccggttatgc gcccgctgtt cctgcattac gaagacgatg cgcacactta caccctgaaa
    14161 tatcagtacc tgttaggtcg cgacattctg atcgctccgg tgcatgaaga aggccgtagc
    14221 gactggacgc tctatctgcc ggaggataac tgggtccacg cctggacggg tgaagcgttc
    14281 cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc
    14341 gatagcgaat gggcggcact gttcgcgtcg ttaaaaagca tctaatcaca tccgcccgtg
    14401 gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca
    14461 actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac
    14521 atcctgctgg atatcggtac actttatctt ttgaagtttt ataccgacgt tctggggctg
    14581 ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat
    14641 atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt
    14701 ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc
    14761 acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga
    14821 ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg ttcccgctat caccaaaaac
    14881 cccaacgaac gcgcctcact ggcggcatgg cgtctgggcg gcgctacgct gggcctgctg
    14941 ctgtgcacgg tgggattcgt gccagtgatg aatcttatcg agggtaatca gcaacttggc
    15001 tatatcttcg ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac
    15061 tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca atccggcgca aaagcctggc
    15121 ctgttgcaat ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgtattgcc
    15181 aacctctgca ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta ttacacccag
    15241 tacgtactta acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt
    15301 ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat
    15361 atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggc
    15421 tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgtt tgttaacagc
    15481 ctgaactggg cgctggtttc cgacaccgtc gagtacggcg agtggcgcac cggcgtgcgt
    15541 tcggaaggca cggtctacac cggcttcacc ttctttcgca aagtgtctca ggcgctggct
    15601 ggtttcttcc ccggctggat gctgacgcaa attggctatg tgccgaacgt cgcacaggct
    15661 gaccacacca ttgaagggtt acgccagttg atcttcatct acccaagcgc actggcggta
    15721 gtcaccattg tggcgatggg ttgcttctac agcctgaacg agaagatgta tgtccgcatt
    15781 gtggaagaga tagaagcccg taaacgcacg gcgtaataat aataaacgac gccctgcggg
    15841 gcgttataag gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg
    15901 gaaaaaatcg cctatggtat gggcgacgtc ggttcgaatt taatgctctg catcggtact
    15961 ctgtatctcc tcaaatttta taccgatgaa ttagggatgc ctgcttacta tggcgggatt
    16021 atctttctgg tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc
    16081 gactcgcgga aaaatattgg gcctaaaggc aaatttcgtc cctttatttt atatgcagca
    16141 gttccggcgg cgttaattgc aacgctccag tttatcgcca ccaccttttg tttgccggtt
    16201 aaaacgacga ttgccaccgc gctgtttatg atgtttggac tttcatacag tctgatgaac
    16261 tgctcgtatg gtgcgatgat cccggcaatt accaaaaacc cgaatgagcg cgcgcagctc
    16321 gcggcttacc gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt
    16381 ccgttgcagt cgcttttttc tgactcaacc gtcggttatg cctgtgcggc acttatgttc
    16441 tccattggcg gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg
    16501 gacacagcgc caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgt
    16561 aatccaccat tgctggtttt atgcattgct aacctgtgta ctctggcggc atttaatatc
    16621 aaactggcga ttcaggtcta ttacacccag tatgtgctga atgatattaa tttattgtcg
    16681 tggatgggat tcttcagtat gggatgcatc ctcgtcggcg tattactggt gccagtaact
    16741 gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca tggtgctgtg ggcggtgggt
    16801 gatatactga attatttctg gggaagtaac tctttcactt tcgtcatgtt ctcttgtgtc
    16861 gccttttttg gcacggcctt tgtaaacagc ctgaactggg cactggtacc agataccgta
    16921 gattacggtg aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc
    16981 ttctttcgta aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa
    17041 attggttatg ttcccaacat cgcgcaaagc gatgcgacat tacagggttt gcgtcagctc
    17101 atttttatct ggccttgtgc actggcaatt attgctgcat tgacgatggg attcttttac
    17161 acactcaatg aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg caaaaataaa
    17221 gagatggcga cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt
    17281 tccattctgg tgggtggcgg cctccctacg tttaaaaaat ggacttatta tgaaaaagat
    17341 taatgcaata attttattat cttctctgac ctctgcctcg gtatttgctg gcgcatacgt
    17401 cgaaaacagg gatgcatata atcttgcctc cgatcagggc gaggttatgc tacgtgtggg
    17461 ttataacttc gatatgggcg cgggtattat gttaaccaat acctacacct ttcagcgaga
    17521 agatgaacta aaacatggct ataatgaaat tgaaggttgg tatcctttat tcaaaccaac
    17581 cgataaatta accatccagc ccggtggctt aattaatgat aagagtatcg gttcgggtgg
    17641 tgcagtgtat ctggacgtca actataaatt tgtaccatgg tttaatctga cagtacgaaa
    17701 tcgctataac cataataact atagttcaac agatttgagc ggggaactgg ataataatga
    17761 cacctatgaa attggcacct actggaattt taaaatcacc gataaatttt cctatacatt
    17821 tgagccacat tatttcatac gcgtaaatga ctttaatagc agcaatggaa aagatcatca
    17881 ctgggaaatc actaatacct tccgttatcg cattaatgaa cactggctgc cttatttcga
    17941 attacgttgg ttagatcgta atgtcgaacc gtaccaccgc gagcaaaacc agattcgtat
    18001 cgggacgaag tatttcttct gatgtgaaac tgccggatgc ggcgtaaacg ccttatccgg
    18061 cctacggttg atacggtttt cgttggccgg attaagcaca tccggccatt aatcaagctt
    18121 cagccgccag catctctttc ttacgttggc taattcgctt aaccagcatt acggaaacca
    18181 ccgcaccaca gaatgcgaag caggccataa tgccaaacac gattttgtag ccaataatcc
    18241 ccggatttaa atccagaatg tagccataca gactgaagca gaacatcgcc ggagcgtaac
    18301 caataaagct acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc
    18361 cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac
    18421 atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg
    18481 cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat
    18541 ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc
    18601 cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga
    18661 atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt aaaagcaata agccagatag
    18721 ttttgtcttt cagcaccgag gtcatactgg tgtttttcga tgctccgtct tcttttttca
    18781 cctcgacgga cggtgcctct tctttgtcat tcagaacaaa gaaaatgata atgcctacgg
    18841 caatcacaat aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa
    18901 accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg
    18961 tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga ctcaccgact
    19021 tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga
    19081 ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga
    19141 gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa
    19201 aacccactgt cgtgacaaat gagtttaccg acatagcatt accaatttga ccattggtca
    19261 aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa
    19321 ttgtaccgcc acacaatgtt aatagactaa ataacgccca tttctttttc gtgagcataa
    19381 catcccctaa gcgattttta aaatcaatgt tttaaaggca ataagatacc gaccattcat
    19441 atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca gaagatggtt
    19501 tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc
    19561 ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggcaatg gtgtttcact
    19621 catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc
    19681 atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag
    19741 gcatataagg tgcgttaata acgctttgaa cggcatatca tcctgacctg aaatacaggc
    19801 tttatgaccc aggccacccg gttctttaac gatcaaggca tctttgatat ggacctgggt
    19861 aatatgcggc gccatcgttt ttaaagcgtc gatgggatgc tcatttgcat tgatcatgtt
    19921 cgcaaaatca aacagtaagg aaagagattc catctcactt tctttgacca gcgacaccag
    19981 ctcatggctt tttaaatctt catgctgttc gatcgtaaaa gtcaggccgc tgtcctgata
    20041 cgtttcccgt acataggcaa tgtcgttagc gatgatcgat aatacgtcgc gcaaattacc
    20101 ttcataacgt ggataaaaac gtacggacga tgccccagtt ttcaacgcaa tggcgacggc
    20161 ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa ggttcagacg
    20221 gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg
    20281 ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag
    20341 taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat aagcatgtaa
    20401 ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcaccat
    20461 aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta
    20521 tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac
    20581 ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca
    20641 atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa aatgagtggt
    20701 tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt tcgtgataga
    20761 aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggtg
    20821 tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt ttctcggtaa
    20881 acccccagga aggttcccgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct
    20941 ccgggctgta attaaatcgc ggctgagtca cgaaccaccc tttacgatcc ttacggaaga
    21001 ttttggattc tgcctcgagg ttcagcaacg cctggcgcag cgtcatacgt ttaatgccca
    21061 gcagctctcc cagctctctt tctgaaggga gttttccacc cggcgtagtg atgccatctt
    21121 ttaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattt cccgtcattg
    21181 tttaatcctt ttggtttaaa ccagatgagg catcttacgc ttacgccaaa aaagaaagca
    21241 attaacggcg tatgactttg cagataattg tgacaaagct ctctattgac gtaaccacag
    21301 acttataagg gagatactcg cattgctgag tgctcacgac aggcaaaaaa aaacctgcca
    21361 tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt gcgcggttgg
    21421 cgcgacgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggggtca
    21481 cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag
    21541 gcggaaccag aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac
    21601 cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata atctgacctt
    21661 cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg
    21721 cgaacgcgac cgctttaccc tgaccgttag agatcagtac gccgttctga cgctgaccca
    21781 cttcacccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg
    21841 aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg atcacgtagt
    21901 cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc
    21961 ccagcgcctg cattacagaa ccctgatgct gttcttcaac gtccagcgtc acgttttcat
    22021 acggctcttg tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca
    22081 gttcgaaacc ttcacgacgc atgttttcga tcagaacaga caggtgcagt tcgccacgac
    22141 cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca acgttatgta
    22201 ccagttcttt gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac
    22261 cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga
    22321 gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc
    22381 caaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg atacgttcca
    22441 gaccgaggtg gcccagcact ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga
    22501 tgatagtgac ctgttggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa
    22561 cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt
    22621 caacgtccgg cgcaggaacg tgatcaacaa tcgcctgata cagcggggtc atgtcttccg
    22681 ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga
    22741 agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa
    22801 cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc
    22861 cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt
    22921 caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt
    22981 cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt
    23041 ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct
    23101 cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg
    23161 tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa
    23221 ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac
    23281 aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg
    23341 gagcataaaa agggttatcc aaaggtcatt gcaccaacat ggtgcttaat gtttccattg
    23401 aagcactata ttggtgcaac attcacatcg tggtgcagcc cttttgcacg atggtgcgca
    23461 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttac
    23521 ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgaccatgac caatccagga
    23581 gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt
    23641 tgttgatttg cgcttcaccg atactaaagg taaagaacag cacgtcacta tccctgctca
    23701 tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg
    23761 ctggaaaggc attaacgagt ccgacatggt gctgatgcca gacgcatcca ccgcagtgat
    23821 tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg aacctggcac
    23881 cctgcaaggc tatgaccgtg acccgcgctc cattgcgaag cgcgccgaag attacctgcg
    23941 ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga
    24001 tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg
    24061 cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa
    24121 aggcggttac ttcccggttc caccggtaga ctcggctcag gatattcgtt ctgaaatgtg
    24181 tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc
    24241 tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca
    24301 gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat
    24361 gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa
    24421 aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta
    24481 ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa acccgaccac
    24541 caactcttat aagcgtctgg tcccgggcta tgaagcaccg gtaatgctgg cttactctgc
    24601 gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat
    24661 cgaagtacgt ttcccggatc cggcagctaa cccgtacctg tgctttgctg ccctgctgat
    24721 ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct
    24781 gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga
    24841 agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcactga
    24901 cgaagcaatt gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac
    24961 tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc
    25021 ggcgcaacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct
    25081 tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac
    25141 taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct
    25201 gggcagatcc tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc
    25261 cattacgcca accctgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt
    25321 acaccgttac cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt
    25381 ctggaggcgg ggcaaggttt taccgataac gaagtgacgc tggtcatcga cgggcgctcg
    25441 catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggct
    25501 ccgatggata accagcgccg cttaagtcag gaacagctac agcacgccca gcaggttgct
    25561 gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt
    25621 ggcgcggcgc agctactcag caaagcgtta cctgacccat cactactcga atataccaaa
    25681 gtgattatcg aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcag
    25741 ctgcccggta cgcgcgttac cgaaagtatt cacaaagtag ctgaacgcgt ggtaacgctg
    25801 gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg
    25861 gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg
    25921 ctacaggcgc tggggccgga gggtggtgaa atcattctgc gtacccgcac cgcgtttcaa
    25981 ctgaccttac acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg
    26041 ccgggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa
    26101 ggtggcaccg ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa
    26161 attgaattta ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa
    26221 taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc
    26281 gttgggtgct tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg
    26341 gcgcggaagt actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc
    26401 gtatgccggg aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc
    26461 ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac
    26521 aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagccgtc gcgctggttg
    26581 agcgcgccat cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc
    26641 caacgaccga tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc
    26701 ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac
    26761 tggtcgctca tgccctgcat cgccacagtc cgcgcgccaa agcgccgttt atcgcgctga
    26821 atatggcagc tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg
    26881 cgtttactgg cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggtacat
    26941 tattcctcga cgaaattggt gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc
    27001 tggcagacgg tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga
    27061 ttatcgctgc cactcaccag aatctcgaac agcgagtgca ggaaggtaag ttccgtgagg
    27121 atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg
    27181 aagatattcc ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag
    27241 aagcgaagtt actgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca
    27301 acgtgcgcca gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag
    27361 tgttgattca ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc
    27421 aaatgcaacc ggacagctgg gcgacgcttc ttgcgcagtg ggcagacaga gcgctgcgtt
    27481 ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga
    27541 ccgcgttgcg acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc
    27601 gcaacaccct gacgcgtaag ttaaaagagc tggggatgga gtgattcaca gcttgtgtgt
    27661 aaagattgat tattgagcgc aaattgctgg tattttacgc tttactgttc cgataagttc
    27721 agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg
    27781 tatcgagtgg cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg
    27841 ccgcaatgat tgggctgttt agttgagttt cagattgctg atgaagtgct ctttgttcat
    27901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc aatattttca acaggttttg
    27961 taggcctgat aagcgtagcg catcaggcaa tcttgttctc agcacattga tggcgctttc
    28021 gttttctact ttgtaaacga agcgccattc actatttaaa tcacccgtga gaactgctgc
    28081 atccgcgctt tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag
    28141 cgacctttcg ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct
    28201 aacggggcga gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt
    28261 ttctcaatag gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg
    28321 cgaatacagt catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatcc
    28381 acttgctgat agtactgctt caactctttc tggttctgcg cgtagcagtc gccaatcatg
    28441 ctgatggcgg aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctgg
    28501 aagttacgat gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc cggacgggca
    28561 aagtgatcca taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc
    28621 aggatatcga gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca
    28681 gcaaaaatgg tcggcagatg cgcgtagtta aagacactca gacgatcggg gttcagctcc
    28741 gccacacgtt tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag gccgtaaatc
    28801 aggtcgatgt tggtggaggt aaaaccaatc tcacgcgcat ggttaagcag tgcaaagatg
    28861 aactcttcat cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg
    28921 cccatgctca ggcgattaaa gccttcggcg cgtaaatgat cgagtacatc cagttcgatt
    28981 tcccgcggat cgacttcgat cgaaatctcc gcatcggcat tgaactggaa gttttcgcgc
    29041 agcagcttca tcaggcggct gatttgcgct ttattcagat acgtcggcgt tccgccgccc
    29101 cagtgcaatt ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc
    29161 tccagcgcgt ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa
    29221 ccgcagaagt aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc
    29281 tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc
    29341 agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc
    29401 agatcccagt cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc
    29461 tacggcgtac cgacggatgc ccgtttcggg tcattagccg gttgcgcagc cacttttgtc
    29521 gccgcgacaa ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg
    29581 ttatcagcag aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca
    29641 tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga
    29701 gtttctgcat caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg
    29761 cactcagggt ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt
    29821 ccagtaattc caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct
    29881 gtttggtgac tttttcagtc acgcccaatg gaataggggt tttactgcca atacgtggat
    29941 cttttggtgc gttctggcct ttgctgcctg acgtggtgtt tccgcccgct gcgcggctgc
    30001 ccggcgcatg accacgacgt tttttctgac gcttgcgatc acgagcttcc tgatccagct
    30061 cctcgcgtgt ttttcgacgc gcttttgcat gacctttgct gcgtgagttt gaagatgatg
    30121 gtttcataat ggttcgtctt ttagccgttt tattcagtat agatttgcgg cggaatctaa
    30181 cagaaagcaa gcaaagaaaa aaggcgacag attactctgt cgcctttttt cctgactcat
    30241 aacccttaac gggtcttaca atccttgcag gccgccaaca caccctgttt ttcctttagc
    30301 cggaacaatc cgtgttgatt ccatttccgt ttaattacgt ctccggacgt ttgtcttcct
    30361 gacaatcctc tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc attgttcctg
    30421 ttagcgtcct cgctctatgg gtgctacttt acgcctttgc tttaaacaaa caatcaaccg
    30481 ccatcagtat ttgcacattt tcagagtttt atataaaagc aattcactga tttatatagc
    30541 ttatttgaca tgcatcgcag aattttctct gctatttcgc ataaagacta tcggcaatgt
    30601 ctcacaaagc gtatggcttt tacttacaac gctcacgccg aaaaatccgc cttttattat
    30661 ggttcaggta taatcgccac aaagctttga ctgacggaga cgaccgcttt gactaatttg
    30721 aattatcaac agacgcattt tgtgatgagt gcgcctgata ttcgccacct accttccgat
    30781 accggaattg aagtggcttt tgcaggccgt tccaacgcag gtaaatccag cgcgctgaac
    30841 acgctgacta accagaaaag cctggctcgt acctcaaaaa ccccagggcg cacccagctt
    30901 atcaacctgt ttgaagtggc tgacggcaag cgtctggttg acttgcctgg gtacggttat
    30961 gcggaagtcc cggaagagat gaagcgcaaa tggcagcgtg cgctcggcga atacctcgaa
    31021 aaacgtcaga gcctgcaagg tctggtggtg ctaatggata ttcgccatcc gctgaaagat
    31081 ttggatcagc agatgattga gtgggcggta gacagcaata tcgccgttct ggtgctgctg
    31141 accaaagcgg acaaactggc aagcggcgca cgtaaagcgc aattgaatat ggtgcgtgaa
    31201 gctgtactgg cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgtt gaagaaacaa
    31261 ggcgtggaca agctgcggca gaaactggat acctggttta gcgagatgca gcctgtagaa
    31321 gaaacgcagg acggcgaata attttcttgc cttaatgctt gtgccggatg tggcgtatcc
    31381 ggcccgtaaa ttcaatagat tacaattcta ccgttggcct gataaacata gcgcatcagg
    31441 cattacggat cttttctttc gcccaataaa aaacgcccca gtcattactg actggggcgg
    31501 ctaaaatatt cagccaaatc cgattacgtg aagtaaaagg tctgaaagat agaacatctt
    31561 acctctgtac cctacgcgac taactttact cttttttgtt cagttcagaa agcacttttt
    31621 gtaatttttt ttcagttgtt gcataggaat ttctaatagc catcacaaaa cgtgacagct
    31681 tatgttgctt acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca
    31741 gttttcgcca ctccccactt ccaccagcaa cggcacatcc agacgggtac agttttccat
    31801 cagttgatga atctgcttcg cgacggcatc aacatcatct ttatgaactt caaataccag
    31861 ttcatcgtgt acctgcatga tcatacgtac acgcggttgc tcagcctgta accacgcatc
    31921 aacggcaatc atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat
    31981 ggctgcacgt tcagccgctg cacgacgagc accattgctg gatttgatat ccggcagata
    32041 cagacggcgt ccgtccagcg tttcaacgta gccctgctct ttcgcctgag cacgggtgcg
    32101 ttccatatac tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc
    32161 ttctttacgt ggaatgttca attgccgcgc cagaccgaaa gcactcatgc cataaatcag
    32221 accaaagttg atcgctttcg cgctacggcg ttgctcgctg gtgacggttt ccagtggcaa
    32281 accaaacact tctgccgccg ttgcccggtg gatatctttt ccttccgcga atgcggtcag
    32341 caagcctttg tcacgcgaaa gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc
    32401 tgagacaatc acataatcct ctggcgcaat aaacgcctgg cggatacgac gaccttcttc
    32461 gttacgcacc ggaatgtttt gcaggttagg atcggttgac gataaacgtc ccgttgcagt
    32521 tactgcctgg tgataagagg tatgcacacg cccggttttc gggttgatca tcagcggcag
    32581 cttgtcggtg taggtcgatt tcagcttcgc cagaccacga tactccagaa tcacttttgg
    32641 caacggatag tccagcgcca gttcttccag tacctcttcc gacgttgacg gcgcgccacc
    32701 cggcgttttc ttcagcggtt taataccctg tttttcaaag agaatggttt gcaactgctt
    32761 ggtggaagaa aggttaaatt cttcacctgc aatttcatgc gctttctttt ccagctcagc
    32821 cagacgcaag gtgagctctt cagaatgatt gtgcagcact ttcggatcga tcttcacacc
    32881 gttacgttca atgcgtgaaa gcaccggcac cagcggcatt tcgatattct cgaagacgtt
    32941 caacggccct ttgtgttttt gcagatctgg ccacattttc agatgcaact gcaaggtgac
    33001 atcagcatct tcggcggcgt aacgtccggc ctcttcgagg gcaatctggt taaaggtcag
    33061 ttgatttttg cctttacccg caatctcttc aaaagtgatg gttttgtgct tcaaccaacg
    33121 ttccgcgagg ctgtccatat cgtgacgccc ggcaacgcta ttgagaatgt aggactccag
    33181 catggtatca aacgcaatcc cacgcagctc aatgccgtaa ttcgccagaa taccgcgatc
    33241 gtatttcagg ttttgcccga ccttcagcgc cttttcatct tccagcagcg gttttagcaa
    33301 ctcgagtgca cgctcgcgag agatttgatc gggcgcgtca agataatcat gagcaaccgg
    33361 aatatatgcc gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt tagcagagat
    33421 gttatcaagg ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc
    33481 aatccacgct ttcagtgttt cttcatcaag gatggtgacg tagttgtcat aagaaatcac
    33541 cgttgccgtc acttctggcg cttcgtctgc aacactggtt tcctgtggct tcgcggctgg
    33601 ttttgcccct ttggcctgta accatttgcc cgcttcgaca tcagcagtcc agcgtttgaa
    33661 ctcatacttt ttgaacagcc ccaacaactc ttccgctgcc ggttgctgca cttccagttg
    33721 ttcacaggtc agctccagtt caacgtcggt tttaatcgtc gccagctggt atgagagata
    33781 agcaacttct ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac ggaagctcaa
    33841 cccagctatt ttttctggct cggcatacag cgtatccagt ccgccaagac cttgcagcaa
    33901 tgcctgcgcg gttttttcac cgacgcccgg tacgccagga atgttatcag aggagtcacc
    33961 catcagcgcc aggaaatcga tgatcagttc tggcggcacg ccgtacttat tcaccacctc
    34021 ttccggtccg aggatggtat tcgtcatggt attgataagc gtaatatttg gcgtcaccag
    34081 ctgcgccata tctttatcgc cagtgctgat cagcaccgga cgcccggctt tttcggcttc
    34141 gcgcgccaga gtaccgataa cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag
    34201 tcccatcgct ttaaccatcg cgtgcaaggg ttcgatttgt gcacgcagat cgtccggcat
    34261 tggcgggcga tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt ttcccttggc
    34321 gtcaaagacc accgctgcat gcgtcggttt atattgcatg atcagactgc gcagcatgtt
    34381 gaggacacca tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg ggggaaacgc
    34441 gtgatatgcg cgataaagat aagatgaacc atctacaagg ataagtggat tttgggggat
    34501 ctgaaccata atgtccgtgc ctgtttatca gattatgggt aaaggatgcc acagacagga
    34561 tgaaaacatg agtttttcac cgcttttcgt gaaaatcttt tgtagatctt ctggatcgct
    34621 cgcaaatcgt catgtggata actttgtgca taaattattt acatcgcttt taatttatta
    34681 tcatgctttt tacagcatga tgtttgtctt tgtttttcat gcagttaaaa ataaatccat
    34741 ccctaataat tgagataatg ggattttttc taatgtggat agatatgaat tatttttctc
    34801 cttaaggatc atccgttatt tgggtcgttt tctgctaaaa tctgcgcctt gcgcttcttt
    34861 agcgcactgt ctatggctaa ccttttgaat aaattcatta tgacgagaat actcgctgcg
    34921 ataacccttt tgctgagtat cgtattgacc attttggtca ctattttttg ttccgtcccg
    34981 atcatcattg ccgggattgt aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt
    35041 tcgcgttttt gtgattttat gatgtattgc tggtgtgaag gtctggcggt attactgcat
    35101 ctgaacccac acctgcaatg ggaagttcac gggctggaag ggttaagtaa gaagaactgg
    35161 tatctgctta tttgtaacca ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt
    35221 cgtaagcaca ttccgatgaa taaatatttc ctcaagcaac agctggcctg ggtgccattt
    35281 cttggcctgg cgtgctggtc gctggatatg ccgtttatga agcgttattc ccgcgcttat
    35341 ttattacgtc atccggagcg acgtggtaaa gatgttgaaa ccactcggcg ttcttgtgaa
    35401 aagtttcgcc tgcatcccac caccattgtc aatttcgttg aaggctcccg tttcacgcaa
    35461 gaaaaacatc agcaaaccca ctccactttt caaaacttgt tgccaccaaa ggcggcaggc
    35521 attgcgatgg cgctaaatgt actgggcaaa caattcgata aactgcttaa tgttacactg
    35581 tgttatccgg acaataaccg tcagccgttc ttcgatatgt taagcggtaa actgacgcgg
    35641 attgtcgtac atgtggattt acagcctatt gccgatgagt tacatggcga ctacatcaat
    35701 gacaaaagct ttaagcgcca ttttcagcaa tggctgaact cattgtggca ggaaaaagac
    35761 cggcttctga cgtctctcat gtcatcacag cgtcagaata agtaagtttt actcgcctct
    35821 gaatgagcag aggcgagtga gtattttaat gaacaaaacg tccggcacga gacataaatt
    35881 cttcttcgct catctcctgt ccgttaaaaa caactttacc cggtgtataa cgcagctgca
    35941 gcgaggcggt attgttgtca atcgtgatta actgaaacat ctgacccaat gtcatcatcc
    36001 cgctgatttg tttatcagcc tgcttttgcg ctttttccgc atccattcct tcagataaat
    36061 taagctgttt tgcggtttct gtgacgacat taagcggtaa cttcacatca aaattgagcg
    36121 atttgatatc tttgtttgtg gatgatgaag atttggctgg gtcggcaata ctgatatcca
    36181 gattggcatt tagttcgccg agtgcgttct tccatcttac cggttgttta atggtcggct
    36241 cacttttttg taataacggt aaatactctt tgaacaatgc agcattcact tcttgcagag
    36301 caacttcatc gtttgctaac tcagggtgta cagcaagctg cttctgcata gcaatgttat
    36361 actggatgat aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact
    36421 caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga
    36481 cattgatata atctttgtct ttaacgcgat ccagcatggt ttttgcaacg aaagcatcga
    36541 tttgggcaac gtcttccccg tgattgataa tgttaatctg attaaatttc gactcgcttt
    36601 caccaaccgg aaatttcttt tcttctaatc gcgccagaga atcaatggta aaactttttg
    36661 ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat
    36721 ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa
    36781 actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga
    36841 atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca
    36901 gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca
    36961 ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaataca actttctggc
    37021 ctggcttgat attgagatcg ggtgcaccgt tatcgaatgt catttgcatt tgaaaacggg
    37081 aattaaaaaa gcctttgtca aaattttgat aacttaacgt catatcgtag gcatgttcac
    37141 ctttcttttt agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgtg
    37201 tacctgtgta ccatgtgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag
    37261 ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttga atatccactt
    37321 atgctgaaat aatgtccagc ggcaggatgc attatcagaa tgtatttata tttattaaat
    37381 aataaaaaaa gcccgtgaat attcacgggc tttatgtaat ttacatttaa ttattttttc
    37441 tcggacagat atttcactgt atcagcatac tgctgaacaa aaacatccat attgctggta
    37501 tccatgccct gcggattcag ctgatattta ccgttaacaa acatcgccgg aacaccacgc
    37561 aattgcacgt cagctgcagc tttttcctgc tgagcgacca gagatttcac cacgaaactg
    37621 ttccacgccg cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata
    37681 tcagatgctg aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtcact
    37741 ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg
    37801 tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc
    37861 ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg gcagaagaaa
    37921 gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt
    37981 ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc
    38041 agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta attgcaaagg
    38101 gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtctgtcgca gccagtaatc
    38161 ttccccggtt aaccacggaa aatttttcgg gaacgcggga tcagcccaac gccgcattag
    38221 ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc
    38281 ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccactg
    38341 ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc
    38401 atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc
    38461 tccatgtagc cgcagaacgg tgaagtcttc ccgccagtgt gctgtaacgg cggcgatcag
    38521 ctcatctgtc gctttcagga atgccgcttt caacccggaa ggtatcagtg tagcgtcctc
    38581 aaacagcttg cgaggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa
    38641 aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg
    38701 atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa
    38761 tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc
    38821 atcatttacc agctgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg
    38881 ttcagggcga taaaatttga cgacaaaacg tcgacgatct tcgtcctgaa attgatagac
    38941 acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa
    39001 tagagcatcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat
    39061 cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg
    39121 cgccgcttac aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga
    39181 agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca
    39241 ttttcagatg atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg
    39301 actctttcgc cagtttctgc aaaaattgca gcaggttaag gtctggctct ttttgccagg
    39361 ctggctggag gagttcaata acttcattca gacgtttaca tttcatagta atgctcctta
    39421 ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa
    39481 tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggcgg
    39541 cgtagataaa ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc
    39601 gcttatgacg cagctctctc acgtcgtggt taatgccaat cgccatcagg aaatctatca
    39661 ggcaagcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg
    39721 aatgctttca gtaatgcagc aggaagcagg cgagtggttt ttgttttgtc cgtgcgatac
    39781 tccttatatt cccgaggatt tagcagcccg gcttaatcat cagcgcaaag atgcgcctgt
    39841 cgtgtgggtc cacggcggtg aacgcgatca cccgactatt gctctggtaa accgcgcaat
    39901 tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg
    39961 tctggctggc ggtcatgcgg ttgatttcag cgataataaa gatgcattta ttaacgtgaa
    40021 tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc
    40081 gcgtggagtg gtaccggaaa aactacgctt ttgaaacaac tgatcccggc attatgcgcc
    40141 agagggatcc gtccagggct gattaagcat acgcaccatg atatggatgt tgataagcca
    40201 ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag
    40261 caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct acagtttctc
    40321 gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa
    40381 gagatcgcaa agattgtgct gtttcgcgat ggagccggac atcgacctga agaattagtg
    40441 atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta
    40501 ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag
    40561 aacggataag caagagtagg ctggatcaga cgcagccagt gtcgcatccg acctttttct
    40621 gcaccagcgc aaaaaggccc agtcttccga ct
//
LOCUS       DAHBHC010000044        38619 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHBHC010000044 DAHBHC010000000
VERSION     DAHBHC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.044"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(10..1368)
                     /locus_tag="M2A35_002707"
     CDS             complement(10..1368)
                     /locus_tag="M2A35_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCB7198991.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1955..4378
                     /gene="pgaA"
                     /locus_tag="M2A35_002708"
     CDS             1955..4378
                     /gene="pgaA"
                     /locus_tag="M2A35_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HCB7198992.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4387..6405
                     /gene="pgaB"
                     /locus_tag="M2A35_002709"
     CDS             4387..6405
                     /gene="pgaB"
                     /locus_tag="M2A35_002709"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCB7198993.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            6398..7723
                     /gene="pgaC"
                     /locus_tag="M2A35_002710"
     CDS             6398..7723
                     /gene="pgaC"
                     /locus_tag="M2A35_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCB7198994.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7725..8138
                     /gene="pgaD"
                     /locus_tag="M2A35_002711"
     CDS             7725..8138
                     /gene="pgaD"
                     /locus_tag="M2A35_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCB7198995.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8188..8976)
                     /gene="phoH"
                     /locus_tag="M2A35_002712"
     CDS             complement(8188..8976)
                     /gene="phoH"
                     /locus_tag="M2A35_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCB7198996.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(9595..10866)
                     /gene="efeB"
                     /locus_tag="M2A35_002713"
     CDS             complement(9595..10866)
                     /gene="efeB"
                     /locus_tag="M2A35_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCB7198997.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(10872..11999)
                     /gene="efeO"
                     /locus_tag="M2A35_002714"
     CDS             complement(10872..11999)
                     /gene="efeO"
                     /locus_tag="M2A35_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCB7198998.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12057..12887)
                     /locus_tag="M2A35_002715"
     CDS             complement(12057..12887)
                     /locus_tag="M2A35_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCB7198999.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            12923..13225
                     /locus_tag="M2A35_002716"
     CDS             12923..13225
                     /locus_tag="M2A35_002716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199000.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPRDINDYS
                     RVERWDCLQRTMCSCRMPRKRRIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(13430..14926)
                     /gene="putP"
                     /locus_tag="M2A35_002717"
     CDS             complement(13430..14926)
                     /gene="putP"
                     /locus_tag="M2A35_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCB7199001.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVAIALAANPENR
                     VLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYE
                     IIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(15085..15294)
                     /locus_tag="M2A35_002718"
     CDS             complement(15085..15294)
                     /locus_tag="M2A35_002718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199002.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15349..19311
                     /gene="putA"
                     /locus_tag="M2A35_002719"
     CDS             15349..19311
                     /gene="putA"
                     /locus_tag="M2A35_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCB7199003.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLARRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(19351..19989)
                     /gene="rutR"
                     /locus_tag="M2A35_002720"
     CDS             complement(19351..19989)
                     /gene="rutR"
                     /locus_tag="M2A35_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCB7199004.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            20277..21368
                     /gene="rutA"
                     /locus_tag="M2A35_002721"
     CDS             20277..21368
                     /gene="rutA"
                     /locus_tag="M2A35_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCB7199005.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            21368..22060
                     /gene="rutB"
                     /locus_tag="M2A35_002722"
     CDS             21368..22060
                     /gene="rutB"
                     /locus_tag="M2A35_002722"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCB7199006.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            22072..22458
                     /gene="rutC"
                     /locus_tag="M2A35_002723"
     CDS             22072..22458
                     /gene="rutC"
                     /locus_tag="M2A35_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCB7199007.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            22466..23266
                     /gene="rutD"
                     /locus_tag="M2A35_002724"
     CDS             22466..23266
                     /gene="rutD"
                     /locus_tag="M2A35_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCB7199008.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLQQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            23276..23866
                     /locus_tag="M2A35_002725"
     CDS             23276..23866
                     /locus_tag="M2A35_002725"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCB7199009.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HSDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            23877..24371
                     /gene="rutF"
                     /locus_tag="M2A35_002726"
     CDS             23877..24371
                     /gene="rutF"
                     /locus_tag="M2A35_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCB7199010.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            24392..25720
                     /gene="rutG"
                     /locus_tag="M2A35_002727"
     CDS             24392..25720
                     /gene="rutG"
                     /locus_tag="M2A35_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCB7199011.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(25803..25976)
                     /locus_tag="M2A35_002728"
     CDS             complement(25803..25976)
                     /locus_tag="M2A35_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCB7199012.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            26349..26945
                     /gene="wrbA"
                     /locus_tag="M2A35_002729"
     CDS             26349..26945
                     /gene="wrbA"
                     /locus_tag="M2A35_002729"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCB7199013.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            26966..27193
                     /locus_tag="M2A35_002730"
     CDS             26966..27193
                     /locus_tag="M2A35_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCB7199014.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(27231..28472)
                     /gene="agp"
                     /locus_tag="M2A35_002731"
     CDS             complement(27231..28472)
                     /gene="agp"
                     /locus_tag="M2A35_002731"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCB7199015.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(28765..30021)
                     /locus_tag="M2A35_002732"
                     /pseudo
     CDS             complement(28765..30021)
                     /locus_tag="M2A35_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            30282..31202
                     /gene="cbpA"
                     /locus_tag="M2A35_002733"
     CDS             30282..31202
                     /gene="cbpA"
                     /locus_tag="M2A35_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCB7199016.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            31202..31507
                     /gene="cbpM"
                     /locus_tag="M2A35_002734"
     CDS             31202..31507
                     /gene="cbpM"
                     /locus_tag="M2A35_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCB7199017.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31600..32199)
                     /gene="torD"
                     /locus_tag="M2A35_002735"
     CDS             complement(31600..32199)
                     /gene="torD"
                     /locus_tag="M2A35_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCB7199018.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(32196..34742)
                     /gene="torA"
                     /locus_tag="M2A35_002736"
     CDS             complement(32196..34742)
                     /gene="torA"
                     /locus_tag="M2A35_002736"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCB7199019.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGVIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(34742..35914)
                     /gene="torC"
                     /locus_tag="M2A35_002737"
     CDS             complement(34742..35914)
                     /gene="torC"
                     /locus_tag="M2A35_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCB7199020.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            36044..36736
                     /gene="torR"
                     /locus_tag="M2A35_002738"
     CDS             36044..36736
                     /gene="torR"
                     /locus_tag="M2A35_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCB7199021.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(36709..37737)
                     /gene="torT"
                     /locus_tag="M2A35_002739"
     CDS             complement(36709..37737)
                     /gene="torT"
                     /locus_tag="M2A35_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCB7199022.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(38196..>38619)
                     /locus_tag="M2A35_002740"
     CDS             complement(38196..>38619)
                     /locus_tag="M2A35_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7199023.1"
                     /translation="AICRSIDLNSWEYVPDHRGEIVYNTETGDAKEITAPGDYPENTT
                     IIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDTAMASISLIQLKLRAGRN
                     LTQAETARLNAALDYIDAVEATDTSTAPDVIWPELPEA"
BASE COUNT         9578 a         9965 c         9480 g         9596 t
ORIGIN      
        1 aatgccgctt tatggtgact cacaaaaatt caccacatcc ctcacaacca ccttattacg
       61 cccggtctct ttcgcctcgt aaagtgcgtt atcagccagt cgatagatct cattcgggtt
      121 taacgcattt tcctgagtca caacagcgcc aatactaatc gtcacttttt gtggtatagc
      181 gtattcagga ttatcgccag ttaaacgctc aacattttcc cgaatccttt ccgctaaagc
      241 tttcgcacgt tcagtatcga tgtccgtcag caagacgcca aactcttcgc cgccgacgcg
      301 cgctaaaagg tcatctggtc gtatgctttt cccaatgata ttcaccactg tttttatcac
      361 ctgatcgcca accggatgcc cccaggtgtc gttgactttt ttgaagtggt cgatatccat
      421 aatcatgacg caataaggcg tttttttggc tgatgctgat tgaactgtca gttcattaaa
      481 aaaataattt ctgttaaata tattggttaa gggatcgcga tgtgcaatgt tctttgttac
      541 tcgtagcgca ctgaatatat ggcacataaa tattaccata acggttaact tactgactac
      601 ttcaatagtg cgactaatat accaagtgga cacgctatac ctactcataa ataacagaga
      661 gatattgtac agtacggcac aaaaagcaat caccgtcact ccattccaga tatcatagcg
      721 taaaccatta aagtatagat taaccgaaag caaggttaac cacatgaaaa tcattatggt
      781 aacatatgag gctttccaaa ccacctgacc attttcattg gttaactcgg caatgtgtaa
      841 attgtagctc tcataatgac tagaaagtat gtgagcaact atcggccccc caaaaacaaa
      901 aaacaaactt atgcacaaag tcattttttt ggaaaataaa ttgcgctgtt ttgtgttttc
      961 actaacttta ttcaccaatg ccagaaatat taatatgcac aaactcatct gacgaaacaa
     1021 ataatagatt gcaatgtcat tttggacaac actgcttgtc aaacgcccct caataatttg
     1081 ctgaatgata atgacggttt taacaaagta aatcaaacaa ctcagaaaag ccaaactaag
     1141 tatacagttt gaaaattgcc tacggtcata aatgaaatac aactgcataa aaataaatgc
     1201 aacagtatcg ataaaaaata aagcaatcat taccatcaga taggttgatc gtggaacaac
     1261 ttcctcaacg cctggctgat taaaccaact attcaccaaa accaaagcaa gcccaaacaa
     1321 taagctcact aatacagtac tactaattct caaatagtct ttttccataa agctacacca
     1381 atctgtaggt tgtagatccc tttctattat taatagatat aagaactgta ctgttgtcaa
     1441 tatgaaattc cgcccacaaa caataccgat gatgatagtt gctgaatgta attattattg
     1501 cctaagtgct ttatacattg tttgcaacaa gccaggcttg aaaatactta taaatggtta
     1561 tagaaaatcc ttataaaaca ttaattcata gatacattag gaataacaat taaatccgtg
     1621 agtgccgtag cgcagccttt catcaggact ttcgaaaaat ccgaaatcat gcatcggaat
     1681 ttactgattt aattatttta atcctaattt attttgaaaa aggcattggg atttatgccg
     1741 tattcctgaa gatcctcatc attggaatgg attttcgggc gagaaaagga ttttatatgg
     1801 acactctgct catcatttct tcttctcatc atcaacaatt cacgtctctc ttccgcgttt
     1861 aataacggat tatgaggtgc aaaaatatct ttcttttcag ttacctgtaa ttagatacag
     1921 agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca gaaaaaggtg
     1981 cccgaaaacc aaatgggctt tgaaacttct tactgccgca tttttagcag cgagtcccgc
     2041 ggcgaagagt gctgttaata acgcctatga tgcattgatt attgaagctc gcaagggtaa
     2101 tactcagcca gctttgtcat ggtttgcact aaaatcagca ctcagcaata accaaattgc
     2161 tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta ccgtttacaa
     2221 ccgctaccgt catcagcaat taccagcgcg tggttatgca gctgtcgccg tcgcttatcg
     2281 taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct ctctggagcc
     2341 gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgctg gtcactatga
     2401 tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag ccaatttact
     2461 cgcagaagcc tatatctata aactggcggg gcgtcatcag gatgaattac gggcgatgac
     2521 agagtcatta cctgaaaatg catctacgca acaatatccc acagaatacg tgcaggcatt
     2581 acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag atattcgcgc
     2641 tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg aaagtgaacg
     2701 ttatgccatt gccgatcgcg ccctcgccca atacgctgca ttagaaattc tgtggcacga
     2761 taacccagac cgtactgccc agtaccagcg tattcaggtt gatcatcttg gcgcgttatt
     2821 aactcgcgat cgttataaag acgttatttc tcactatcag cgattaaaaa agacggggca
     2881 aattattccg ccctgggggc aatattgggt tgcatcggct tatctcaaag atcatcagcc
     2941 gaaaaaagca cagtcaataa tgaccgagct cttttatcac aaggagacca ttgccccgga
     3001 tttatccgat gaagaacttg cggatctctt ttacagccac ctggagagtg aaaattatcc
     3061 gggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc ttcggttaat
     3121 gggcacgcct acgagcatcc cgaatgatac ctggttacag gggcattcgt ttctctcaac
     3181 cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag agcttgctta
     3241 taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac aagcccgcgg
     3301 ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc cacgtaatat
     3361 taatctggag gttgaacaag cctggacagc attaacgtta caagaatggc agcaggcagc
     3421 tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg tacgattaaa
     3481 acgtgcggtt gatgtacata atcttgcaga gcttcgtatc gctggctcaa caggaattga
     3541 tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg tttattcacc
     3601 accgctgaag gataactggc gcggttttgc tggattcggt tatgccgatg gacaatttag
     3661 cgaaggaaaa gggattgttc gcgactggct tgcgggtgtt gagtggcggt cacgtaatat
     3721 ctggctcgag gcagagtacg ctgaacgcgt tttcaatcat gagcataaac ccggcgcgcg
     3781 cctgtctggc tggtatgatt ttaatgataa ctggcgtatt ggttcgcaac tggaacgcct
     3841 ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca gtgctcaggc
     3901 ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg ctttcactga
     3961 tttttccgac agtaaccagc gtcatgaagt ctcacttgag ggtcaggaac gcatctggtc
     4021 ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa atacagaaca
     4081 cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat ttgaggcaag
     4141 ccatttgtta tggcgaagct atgaaaatag ctgggagcaa atattcagcg caggtgttgg
     4201 tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct acgggcaacg
     4261 cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa aacgacctta
     4321 tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca gattttaagg
     4381 ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat aattatgctc
     4441 accgcgtgca ttagccagtc aagaacatca tttataccgc cacaggatcg cgaatcttta
     4501 ctcgccgagc aaccgtggcc gcataatggt tttgtagcga tttcatggca taacgttgaa
     4561 gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg tgaacaattt
     4621 gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg tgaagcacat
     4681 cgaggaggaa aaccgctacc ggaaaaagct gtagtgctga cttttgatga cggctaccag
     4741 agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc tgtatgggcc
     4801 cccgtcggca gttgggtcga tacgccagcg gataaacaag taaaatttgg cgatgagttg
     4861 gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg ttcccggctc
     4921 gttgagctcg cttctcatac atggaattct cactacggta ttcaggctaa tgccaccggc
     4981 agcttattgc ctgtatatgt aaatcgtgca tattttactg accacgcacg gtatgaaacc
     5041 gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga atacctgcgt
     5101 acaaaggttg aggtaaatcc acacgttttt gtttggcctt atggcgaagc gaatggcata
     5161 gcgatagagg aattaaaaaa actcggttat gacatgttct tcacccttga atcaggtttg
     5221 gcaaatgcgt cgcaattgga ttccattccg cgggtattaa tcgccaataa tccctcatta
     5281 aaagagtttg cccagcaaat tattaccgta caggaaaaat caccacaacg gataatgcat
     5341 atcgatcttg attacgttta tgacgaaaac ctccagcaaa tggatcgcaa tattgatgtg
     5401 ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc atttgctgat
     5461 cccgatggtg atgggctggt caaagaggtc tggtttccaa atcgtttgct accaatgaaa
     5521 gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt aaacatctat
     5581 gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt aaaatactta
     5641 ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct ctctcctttc
     5701 gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg acatgctgct
     5761 tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga tgccagtgca
     5821 ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga aattcgacaa
     5881 aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac tgacttcact
     5941 ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac tgcacgaaat
     6001 atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca gaattatgct
     6061 gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatct ggaaggtgtc
     6121 gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa aaacatccct
     6181 caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa aaatggtcag
     6241 catcaggcta tttcttcgca acaactcgct cactggatga gcctattaca actgaatgga
     6301 gtgaaaaact atggttatta tcccgacaat tttctgcata accaacctga aatagacctt
     6361 attcgtcctg agttttcaac agcctggtat ccgaaaaatg attaatcgca tcgtatcgtt
     6421 ttttatatta tgtctggtgt tatgcatacc cctatgcgta gcgtactttc actctggtga
     6481 actgatgatg aggttcgttt tcttctggcc gttttttatg tccattatgt ggattgttgg
     6541 cggcgtctat ttctgggtct atcgtgaacg ccactggccg tggggagaaa acgcaccagc
     6601 tccccagttg aaagataatc cgtctatctc cattatcatt ccctgtttta atgaggagaa
     6661 aaacgttgag gaaaccatac acgccgcttt agcacagcgt tatgagaaca ttgaagttat
     6721 tgccgtaaat gacggttcaa cagataaaac ccgtgccatc ctggatcgca tggctgcaca
     6781 aattccccat ttgcgggtca ttcatctggc gcaaaaccag gggaaagcca ttgcgcttaa
     6841 aaccggagct gccgcggcga aaagtgaata tctggtgtgc attgatggcg atgcgttatt
     6901 agaccgcgat gcggcggcat atattgtgga accgatgttg tacaacccgc gtgtgggtgc
     6961 cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg gtgggtaaaa ttcaggttgg
     7021 cgagtattcc tcaattattg gtttgatcaa gcgaacccag cgtatctatg gaaacgtatt
     7081 taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc ctggcagaag tgggttactg
     7141 gagtgacgat atgatcaccg aagatattga tattagctgg aagctgcagt tgaatcagtg
     7201 gacgattttt tacgagccac gggcgctgtg ctggatatta atgcctgaaa cgttaaaagg
     7261 gctgtggaaa cagcgcctgc gctgggctca gggcggtgca gaagtattcc tcaaaaatat
     7321 gacaaggttg tggcgcaaag aaaactttcg aatgtggccg ctgttttttg aatactgcct
     7381 gacgacaata tgggccttca cctgcctggt cggtttcatt atttacgcag tccaacttgc
     7441 cggtgtaccg ttaaatattg aattgacaca tatcgctgcg acacatactg ccggaatatt
     7501 attgtgtacg ttatgtttac tgcaatttat tgtcagcctg atgatcgaga atcgctatga
     7561 gcataatctg acttcatcgc ttttctggat tatttggttc ccggttattt tctggatgct
     7621 gagcctggca acgacattgg tatcatttac acgagtcatg ttgatgccta aaaagcaacg
     7681 cgcccgttgg gtaagtcccg atcgcgggat tctgagaggt taatatgaac aatttaatta
     7741 ttacgacccg acaatcacca gtacgtttac tggttgatta tgttgccaca accatcttgt
     7801 ggacattatt tgcgttgttc atattcttat tcgccatgga tctgctgacg ggttattact
     7861 ggcaaagcga ggccagaagc cgacttcagt tctatttttt gctggcagtg gcgaatgccg
     7921 tcgtgttaat tgtctgggcg ctgtacaata agctgcgttt tcaaaaacag cagcatcatg
     7981 cagcctacca atatacgccg caagaatatg cagagagctt agcaatacct gatgagctct
     8041 atcagcaact acaaaaaagc cacaggatga gcgtacactt caccagccag gggcaaataa
     8101 aaatggttgt ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt
     8161 cgcccgggct ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag
     8221 tgccgaacgt acgcagtcct ctttaccgaa ccggacgatc ccaaccattt catcttcttc
     8281 gaaacgttcc agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt
     8341 gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat
     8401 ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg actgcatttt caaaggtacg
     8461 tccacgcata taggcgaacg gcgcaatttc caccttccct atttccggtc gcaggcagta
     8521 ctgcataaag gaagccccta agcgccggac cagcacgtcg tagaccgggc gaaaataggg
     8581 agcaaacttt tctgcgatat ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac
     8641 tggacgggtg acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc
     8701 tgcgctgatc caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact
     8761 ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc
     8821 gcgactgtcg cgggccatac caatggcttc tacgccgccc atctgcacaa gcgaggtgac
     8881 cgattcttct tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc
     8941 acgacgagct ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta
     9001 ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc
     9061 ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc
     9121 ggcgtcggta acacgagtaa agagaggaag tgagaaaaat acagagtgac gaagttgcta
     9181 ccggaggaga aatctccgca agtggtggag cgtagcttaa caagacattg tccagaacag
     9241 gacgttacca tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct
     9301 acttaataat tacaacagat ctcgcattca ttgcaacaat atattcactt atatttaacc
     9361 ataaaacacc atttcagtga cattagtttc tactggaaag atgacagagt gatgacagat
     9421 atgaaaaaag ctgtgtgctt tcagcaggat tcgcatcagg catctgcgca cgaacgccgg
     9481 atgcggcgtg aacgccttat ccgcctaccg aacccgcacc gattgtaggc ataagaagac
     9541 gcgcaagcat cgcatcaggc atctgcgcaa gaacgcctta tccgtctata aacattaaac
     9601 ccgcaataac gcgcttccga gataatcgtt cgcgtccttc actcccggca gcgcaaaaaa
     9661 ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga gccttttttg
     9721 tacagtcagg aagccttttt ccagatcgtg ttggtaacag acaaacagta gccccatatc
     9781 cagttgcccg gagttggtga cgcccagtga ataactgtag ccacgacgca gcatcaggct
     9841 ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca gcgcaatcac
     9901 cttcccttcc ggatcgctgg cgtaatcggg aacatcatgc tcatgctgca ttcccagcgg
     9961 cgcaccggtt tgcttatcac gaccaaaaat cgtctgctgt tctttcagcg gagttctgtc
    10021 ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac cgattgtcca
    10081 cgctggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact tatcattctg
    10141 gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg tctctttgcc
    10201 tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca cgctgagcaa
    10261 atctggcgta tgtttgatga tatcgcgcag cgcatggatc accgtgtcct gggtgttggc
    10321 gcaaatttgt agcaacacat caccatgaca taacgccgca tccagcgagt cgttgggaaa
    10381 acgcgtcatc ttctgcaact ttttcggcat ctgtggcgca aggccaaagc gctcatcaaa
    10441 caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt agccgccaag
    10501 aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgccg ctccgccctg
    10561 agtcagaaaa gcaaaacgct gagtcaacaa gcgaaacaac cgttcaagat cgggtttatc
    10621 gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg tcaggatccc
    10681 tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg aaagcgtacc
    10741 cggcgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca gcgccagcgc
    10801 accaatgcct ttcagtaaac gtcggcgtga cggttcattc acgccgtttt tatcttcata
    10861 ctgcataatg cttaatccaa tcccagcaca ccgcgaagtt gcgccagatc ttccgccagc
    10921 gcagtaatcg gtcctttcag tgcattccgg tcggcatcgg tcaatttgtc gtaggtttca
    10981 aaaccgtctt tagtacggta tttcgccaga atggtatcga cctttttaaa gttggcatcg
    11041 acttttgcca acagttccgg gttggctttt tgcagttgtg gacgcagcag atcgacaatt
    11101 ttctgcgagc cttcaacgtt agcctggaaa tcccacagat cggtgtggct gtagcgatct
    11161 tcttcaccgc tgattttgct ggctgccact tcctcaatca gtccggctgc accgccgacc
    11221 acttttgaag gtgggaaagc cagttcactg atgcgttttt gcaaatcgac cacatcggta
    11281 taaagctggt cagcgtactg atccatgcct ttggtggtgt tgtcgccaaa caatgctttt
    11341 tccagacggt ggaaaccggt gaatttcgga tcggcggctt tttgctcgta atcatcttca
    11401 cgggcgtcaa tgctgccatc cagatcggag aacagttcag caatcggttc gatgcgctca
    11461 tagtgctggc gcgtcggcgc atacagtgct ttcgcttttt cgatatcgcc tgctttaata
    11521 gcgtcagtaa aggctttcgt gtcggtcacc agctgcgtgg tttccgccat gacatacgct
    11581 ttatatgcag taattgcacc accaagactt aacagcgcat cactttgcgc cgcatccgcc
    11641 gttgcctcac ctttgacgat caacttccct ttcgggttag tcagcagacc gcaggtcata
    11701 tcgtattcgc caggctgtaa attcgccgtc attttctggc taaagccagg ggcgatattt
    11761 tcccgctctt ccaccaccat cacgcctttg aggatctccc actccagcgc cttctggctg
    11821 tggttctgaa taatgaactg tgttttcccg gcgttaaccg taatggtcat cggttcgcac
    11881 tgcttatcgg tcacggtcac cttgacctgc ggcacattag cggcgttagc cataaaagca
    11941 gaagagaaca gcgcagccac gctcaactgc aatgcgttac ggcggaagtt aatggtcatg
    12001 acgagactat ccctttaaag tatgttgtaa ctaagcaaga gtttgcgtcg tatttgctac
    12061 gcggagcgag acgctgtcgc ccctgcgcgt ggtggcaggg caaatgccac cagcgccggg
    12121 atgagataaa taaaccagac ggcgacttcg ctgacgctcg gtgcttcctg atagccaaaa
    12181 atcccttcca tcagcgtgcc aaacagcgag tgagttgaga ggaccgcgct catatcgaag
    12241 gcgatttcct gaaagtggtt ccacaatccg gcttcatgaa atgcgcgaat ggcaccagcc
    12301 gccagccctg cggcgacgaa gagaataaac aggctggtcc atttaaaaaa tgcaccaaga
    12361 ttgaggcgaa taccgcccca gtagagcagg aagccgagca ccacggcagt agcaagaccg
    12421 agcattgcac ccagcggcgg ccagatcccg acatcttgtt gaaatgccgc cagcaggaaa
    12481 aagaccgact ccagcccttc ccttgcaacg gcaaaaaaga ccatcatcac cagcgcccag
    12541 ccatgatgat ttccacgctg caatgcgcta tcgactgcct gttccagttg cactttgacg
    12601 ttgcgcgaca ctttgcgcat ccagaaaacc atccaggtaa ggatcaccac ggcgatcacc
    12661 gccacgatac cttcaaacag ttcctgttct ttttgcggaa attcgccggt ggtttcgtta
    12721 atgaagatac ccaggcccag gcacaacgca gcggcaagca acacgccaat ccacatcaca
    12781 ccaatccatc ggcctcgctg ggtacgctta agatagctgg caatcaaact gacaatcagc
    12841 gcggcttcaa gtccttcgcg caacataatg agaaacggaa caaacatgcc acccaccctt
    12901 aaacgttttt ctgagtcaat ctatgcaaag aaaagtaaat cgcagtgata gcgattatca
    12961 ttacggtaaa gaaaaataca agcggaatgt ttataaaatg atgttggaat gtaaacaaag
    13021 agaggtaatg ccaagggata taaacgatta ttccagggta gaaagatggg attgtttgca
    13081 aagaaccatg tgttcttgcc ggatgccgcg taaacgccgt atccagccga caaaactgtc
    13141 cagactcaat gcatcagagc ttccatgtaa aactgataag cgggtgtatc aagcaatttc
    13201 gtccatccgc gattatcaaa tctaactttt tgcccggaac ataataacag ctcgggtgtt
    13261 ggtggcgtca aaatcgctga agcgttcccc gaaggccggg acagcagggg gcggcggcga
    13321 ttggccgccc cctgcgcgct ccctgcacca ttcaatttat atggcgaggc acattaaggg
    13381 agcgcaaccc gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagctttcct
    13441 gcaaccgtga cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca
    13501 tcgccgctga cggcgcttta cccagcaaac taaacactac aatcccaata ctgccgaaga
    13561 taaagcccgg aataatttcg tagagtccca gccagccgaa ctgcttccag acgataaccg
    13621 tcagcgcacc gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt gaccacatca
    13681 ccgagaacag caccaccgga ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta
    13741 agcccagcac gcggttttcc gggtttgccg ccagcgcaat cgccaccagc accatcacac
    13801 gccctaccca caccagctct ttctggctgg catgtttacg cagaaacgct ttgtataaat
    13861 cttcggtaat cgcactggag cacaccagca gctggcagct taacgttgac attaccgccg
    13921 ccagaattgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    13981 cgataaacac acgttcagcg ttctggttta ccgcacctgc taccgccggg tgctcattaa
    14041 agtaagcaat cccaaagaag ccgacagcca ccgcccctgc caggcagagg atcatccagg
    14101 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atccgccgcc ataaaacgcg
    14161 ccagaatatg aggctgaccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    14221 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    14281 gcgagtcacc aaagccaccg acactgataa tgacgataac cggtgtcagg atcagggcaa
    14341 aaatcatcag gctggcctgt acagtatcag tccagctcac cgcgaggaaa ccgccaataa
    14401 aggtgtaaag gatcgtcgcc gcagctcccg cccacagagc cgtttcgtag ctcatgccaa
    14461 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    14521 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaagcgcc
    14581 cggtgaaata atccggcagg gttaaggcgt tattgttgta ttcggtatgc acacgcaacc
    14641 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    14701 tttcggaaat cccggaaaga aaaaccgcgc ccggcaaccc cattaacagc cagccgctca
    14761 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    14821 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    14881 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    14941 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    15001 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    15061 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    15121 ctctctcaca ttttttgcgg ttgcaccttt caaaaatgtt aactgccgca gagaaaaagt
    15181 ctgatttatt tttttaatcc ctgtcatatc gatttctttt attaacattt cattcatttt
    15241 taagcttgct acgcaggtca catttaacat ggttgcacaa agttgcaaca tcatggatat
    15301 ttcacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
    15361 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
    15421 atcgatcgca caccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
    15481 gaaaacagcg atactctgcc agagctacct gcgctgcttt ctggcgctgc caatgagagc
    15541 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
    15601 attttgcccc agtcggtttc ccgcgccgcg atcaccgcgg cctatcgtcg cccggaaacc
    15661 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc agccagttgc tgcacaggcg
    15721 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggtcgc
    15781 gcaggtatgg tccaggggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
    15841 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg cgacgcgtta
    15901 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
    15961 tttgttaatg ccgccacctg ggggctgctg tttactggca aactggtttc cacccataac
    16021 gaagccatcc tctcccgctc gctgaaccgc attatcggta aaagcggtga accgctgatc
    16081 cgcaaaggtg tggatatggc gatgcgcctg atgggcgagc agttcgtcac gggtgaaacc
    16141 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
    16201 gatatgctgg gcgaagctgc actgactgcc gcagacgcac aggcgtatat ggtttcctat
    16261 cagcaggcga ttcacgccat cggtaaagcg tctaacggtc gtggcatcta tgaagggccg
    16321 ggcatttcaa tcaaactgtc ggcgctgcat ccgcgttata gccgcgccca gtatgaccgg
    16381 gtaatggaag aactttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtatgat
    16441 attggtatta acatcgatgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
    16501 gaaaaactct gtttcgagcc ggaactggca ggctggaacg gcatcggttt tgttattcag
    16561 gcttatcaaa aacgctgccc gttggtgatt gattacctga ttgatctcgc cacccgtagc
    16621 cgtcgccgtc tgatgattcg tctggtgaaa ggcgcgtact gggatagtga aattaagcgt
    16681 gcgcagatgg acggccttga aggttatccg gtttataccc gcaaggtgta taccgacgtt
    16741 tcttatctcg cctgtgcgaa aaaactgctg gcggtgccga atctaatcta cccgcagttc
    16801 gcgacgcaca acgcccatac gctggcagcg atttatcaac tggcaggtca gaactactat
    16861 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccactgta cgagcaggtc
    16921 accgggaaag ttgccgacgg taaacttaac cgaccgtgtc gtatttatgc cccggttggc
    16981 acacatgaaa cgctgctggc gtatctggcg cgtcgcctgc tggaaaacgg tgctaacacc
    17041 tcgtttgtta accgtattgc cgacacctct ttgccactgg atgaactggt cgccgatccg
    17101 gtcactgctg tagaaaaact ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa
    17161 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
    17221 gctaacgaac accggctggc ctcgctctct tctgccctgc tcaatagtgc actgcaaaaa
    17281 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
    17341 aaccctgcgg aaccgaaaga tattgtgggc tttgtgcgtg aagccacgcc gcgtgaggta
    17401 gaacaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac tcctccggtt
    17461 gaacgcgcgg cgattttgca tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg
    17521 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgc
    17581 gaagcggtcg attttctcca ctactatgcc ggacaggtgc gggatgattt cgctaacgaa
    17641 acccaccgtc cattagggcc tgtggtgtgt atcagtccgt ggaacttccc gctggctatt
    17701 ttcaccgggc agattgctgc cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca
    17761 gaacaaacgc cgctgattgc cgcgcaaggg atcgccattt tgctggaagc gggtgtaccg
    17821 ccaggcgtgg tgcagttgct gccaggtcag ggggaaaccg tgggcgcgca actgacgggt
    17881 gatgatcgcg tacgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
    17941 cgcaatatcg ccagccgcct ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc
    18001 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaggt cgtcgtggat
    18061 gtactggcct cggcgttcga cagtgcgggt cagcgttgtt cggcgctgcg cgtgctgtgc
    18121 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggcgcaat ggccgaatgc
    18181 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga tagcgaagcg
    18241 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
    18301 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
    18361 acgctgatcg aactggatga ctttgccgaa ttgcaaaaag aggtctttgg tccggtgctg
    18421 catgtggtgc gttacaaccg taaccagcta ccagagctga tcgagcagat taacgcttcc
    18481 ggctatggtc tgacgcttgg tgtccatacg cgtattgatg aaaccatcgc ccaggtcact
    18541 ggctcggccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggtgggt
    18601 gtgcagccat tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
    18661 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcagtgac gctcgcgcgt
    18721 caggatgcag agtatccggt cgatgcacag ttgaaagccg cattgactca gccgctaaat
    18781 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
    18841 gagctggcgc aggcaggaac acaacgattg ctgccggggc cgacgggtga acgcaacacc
    18901 tggacgctgc tgccgcgtga gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg
    18961 actcagctcg ccgccgtgct ggcggtgggc agccaggtac tgtggccgga tgacgcgctg
    19021 catcgtcagc tagtaaaggc attgccatcg gcagtcagcg aacgtattca actggcgaaa
    19081 gcggaaaata taaccgctca accctttgat gcggtgatct tccacggtga ttcggatcag
    19141 cttcgcgcat tgtgtgaagc cgttgctgcg cgggatggcg caattgtttc ggtgcagggt
    19201 tttgcccgtg gcgaaagcaa tatccttctg gaacggctgt atatcgaacg ttcgctgagt
    19261 gtgaataccg ctgccgctgg cggtaacgcc agtttaatga ctataggtta aacaggtagc
    19321 cggaggatgt tacaacctcc tccgacatct ttaacgtggt cgaatcccct caataataat
    19381 ccgctgcacg ttttcaaccg tttgattgaa aaatatctca tcgcgcaacg tcgcgcctgt
    19441 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcatgaa
    19501 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
    19561 cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca ttaacagcgg
    19621 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgggagac
    19681 ttccagcttc agacggatgt actcttttat cgccgccagc ggagcgaaat cttcacgaaa
    19741 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
    19801 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcgat
    19861 ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
    19921 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
    19981 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
    20041 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
    20101 cgtttagtcc actttttacc agatatttta gataacgcat tgttaacata ttaacaacat
    20161 taacttttta aaaactggca tccgctttgc gaacaagcca ttacgcggct gaaaaggata
    20221 tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
    20281 aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc cacgcgccgc
    20341 agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag cactaccatt
    20401 tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact gagttctggg
    20461 atcacaacct tgagtcgttc accttgatgg cggggctggc ggccgtcacc tcgcgcattc
    20521 agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc cgtatggccg
    20581 caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact ggctggcaaa
    20641 agcccgagta tgagcagatg ggcatctggc ctggcgatga ctatttctcc cgtcgttacg
    20701 actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg aaaagcgatt
    20761 ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg agtgtcccca
    20821 tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc cagtatgccg
    20881 atttcaactt ctgtttcggc aaaggcgtaa atacgcccac ggctttcgcc ccgaccgctg
    20941 cgcggatgaa acaggccgca gagcaaaccg ggcgcgacgt tggttcttat gtgttgttta
    21001 tggtgattgc cgatgaaacc gacgatgccg ctcgcgccaa atgggaacac tacaaagcgg
    21061 gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat acccgctccg
    21121 gtactgatac caacgtccgt cagatggccg atcccacttc ggcggtaaac atcaatatgg
    21181 ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc gcaagcgtgc
    21241 ctggtgccga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc gaaaccttcg
    21301 gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct acctgcgctg actcaggagg
    21361 tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc cgcagcaaag
    21421 tgcgctgatc gtggtggata tgcaaaacgc ctatgccacg ccaggcggct acttagatct
    21481 cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa ccgctgtgac
    21541 cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg atgaacagta
    21601 tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc tgaaaaccat
    21661 gcgtaatcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg attatcaact
    21721 ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc gctacagcgg
    21781 tttcttcaat acaccgctgg acagcatttt gcgcagccgt ggaatacgcc atctggtttt
    21841 caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct tttttctgga
    21901 gtatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat ttgcacagaa
    21961 agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg aaacgttctg
    22021 cgacgcgctt tctcccacat cctttgctcg tatcgcttaa ggagtttaac gatgccaaaa
    22081 tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc cggcacgctg
    22141 gctgatggcg tggtgtatgt ctccggtacg ctggcttttg atcaacataa taacgtgctg
    22201 tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg caaggtgatc
    22261 gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat taccgactgg
    22321 aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga taaaccggcg
    22381 cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat cgccacaatt
    22441 gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc cttatgctga
    22501 tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact ggttacagca
    22561 actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg gcaccggcaa
    22621 taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg aactgcatca
    22681 ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc tcggtgcgct
    22741 ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga tcagcgttaa
    22801 cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg aacgattact
    22861 gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct atcccgccga
    22921 ctggatggcg gcccgcgcac ctcgccttga ggcagaagac gcgctggcac tggcgcattt
    22981 tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg actttagtca
    23041 ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg atctgctggt
    23101 gccaacagca tgttccagtg aacttcatgc cgccctgccc gatagccaga aaatggtgat
    23161 gccctatggc ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg ctctgttact
    23221 caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa ttgctatgaa
    23281 cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca ctcacaacgg
    23341 ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc tgatgaaatg
    23401 ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca ctgcagaagg
    23461 aaaagaacgt ctgcgcccgg cgctttccag cggcaatcta caaaaaaccc tgaccgcgcc
    23521 cgtcaccgct atcgtcgcct gggacagtga attttatgaa cggttaccac tactgtttcc
    23581 ccacagtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag aaacagcgtt
    23641 tcgcaacagt tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc tgggactgga
    23701 taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt ttgcgggcag
    23761 cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca aactttttgc
    23821 gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag caaactatga
    23881 acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg gcggtcaata
    23941 tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc gtctgcagcg
    24001 tgaccgatac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc gtctggccgg
    24061 tattcaatga aaatcgaacg ctgtgtgtaa atacacttag cgccgggcag gagccgcttt
    24121 ccaacctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc cgctggcaga
    24181 cgggtgtgac cggatgcccg caactggaag aggccctggt ttcgtttgac tgccgtatca
    24241 gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa gcgattcatc
    24301 gtcacgccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg ctgatgcgcc
    24361 ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc ctcactggca
    24421 gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacggt tgccgtttgc
    24481 tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga cggtgttaat
    24541 gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga tcggcacgtt
    24601 actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca gcgcggcttt
    24661 tgtcggcgtg gtgatcgccg cgacgggctt taacggtcag ggcatgaacc cgaatatcag
    24721 catcgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg gcctggtggt
    24781 gatgaaaatc ggtacacgct ggattgaacg gctaatgccg ccggtggtga cgggtgcggt
    24841 ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat cggcttcggc
    24901 ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg cggtgtttac
    24961 ccgaggcatg atccagcggt tattgattct cgtaggatta attgtcgcct gtctgctgta
    25021 tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc tcgtcagcca
    25081 tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatggtc aggcaatgat
    25141 gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc tcaaggcggt
    25201 cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg tcggtgatgg
    25261 tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct atgctgaaaa
    25321 tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg ctgctgccgt
    25381 catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata ccattccggc
    25441 cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg caggcgcaag
    25501 gatatgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta tggtcgcagt
    25561 aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta cgttgggagg
    25621 gattggtaca gcaacctttg gcgcgatttt actcaatgcg ttgttaagca gaaagttggt
    25681 tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc gccgggatta
    25741 ccggcggtga tgactttttg acgagcctaa gaaaaaaatg cattcgtcat tgattagccc
    25801 ggctagttgt cgcttttgcc gtgactgctc ttgccacctt ttttacctgc ttcagatgcg
    25861 cgctgcgggt cattcttgaa attacccccg ctgtgctgtc cacctttttt acctgcttct
    25921 gatgctcttt cgcggtcttc tgcaaaattg ccggaaccgc ctcgatggtt tgccattact
    25981 gacctccgtc tgggtataga catcatattg catagaatta acgcggcgta tggcggctgc
    26041 catattttcg ctacgtggga ctaagcttag tgaactgacg cgggcagacc cgcgtttggt
    26101 aatatcctgc aacagcttct tagcgagaat agatatcatc tcaaataatt atccataagc
    26161 cgctcttatg attaaaaaaa cgcgatgaga taaaaatgtg tcattttgcg acaaaattac
    26221 gtgcttgtga aagttgttat aaatcaaata agtggttgtg aaatttgcac tctgaaaagg
    26281 acgtcttatc tttaaataag tggtagcgaa tcgctacgga atagagataa cacgaggagt
    26341 ggttagaaat ggctaaagtt ctggtgcttt attattccat gtacggacat attgaaacga
    26401 tggcacgcgc agtcgctgag ggtgcaagca aagtcgatgg cgcagaagtt gtcgttaagc
    26461 gtgtaccgga aaccatgccg ccgcaattat ttgaaaaagc aggcggtaaa acgcaaactg
    26521 caccggttgc aaccccgcaa gaactggccg attacgacgc cattattttt ggtacaccta
    26581 cccgctttgg caacatgtcc ggtcaaatgc gtaccttcct cgaccagacg ggcggcctgt
    26641 gggcttccgg cgcactatac ggaaaactgg cgagcgtctt tagttccacc ggtactggcg
    26701 gcggtcagga acaaactatt acttcaacct ggacgaccct tgcgcatcac ggcatggtaa
    26761 ttgtccccat tggctacgca gcgcaggaat tatttgacgt ttcacaggtt cgcggcggta
    26821 cgccgtacgg cgcaaccacc atcgcaggcg gtgacggctc acgccagcca agccaggaag
    26881 aactgtctat tgctcgttat caaggggaat atgtcgcagg tctggcagtt aaacttaacg
    26941 gctaatcttc aacaggagga tacgcatgcc aactcaagaa gcgaaagctc atcacgtcgg
    27001 tgaatgggca tctctgcgca atacgtcgcc ggaaatagcc gaggccattt tcgaagtcgc
    27061 cgggtacgac gaaaaaatgg cggaaaagat ttgggaagaa ggtagcgatg aagtgttagt
    27121 caaagccttt gctaagaccg ataaagattc gcttttttgg ggcgaacaga ccatcgaacg
    27181 taaaaacgtt taaccagcga ctcccccgct tctcgcgggg gagttttctg ttatttcacc
    27241 gcttcattca acacgctatc aaatttatcc atcgggcaga aaccgttggc atctatcggg
    27301 caaccgctta attccagcgt cacacgctga gcaggtgcct gcagggttaa cgcatcggca
    27361 ttacgtaact gttccgcact ctgatacaca tattcaattt tcatcaaatc gcgattggct
    27421 ttgctgtcat gccaacgctg gaaaacgatt ttgccgccaa tcggcgtgcg ttcgttctgg
    27481 tcatgcaact gatacggttt gaaatccagc gccgttaaca gagaggcaat gttggagtcg
    27541 tgcccaacca acactgtaat tttcggtgcg ctggtgcgat cggtgaccag agctttgtcg
    27601 atataactga ccagcggttt cgcaacattg cgcgccactt ccggtgaggt aaacaggctg
    27661 tcctggtagc cgtttttaag cttcgacaac accttccact gctggtcaga tttgatttct
    27721 ccccaggcca cctgatccat cggaaaacct tcgtaatatt gcaaagtaaa tgcatctacc
    27781 agcgagttgc cgactttcag cggcccggaa acacctggtt cttgttgata cttggcgcta
    27841 aaggtatttt tgccatccac cagcgaacac tgttgtttct ctttacaggc aggggaatct
    27901 ttatagttaa cgattttttc cagtagctgg tagctgtcgg taagctggag tttgctgagc
    27961 tctttctcca ttgccgccac cgcctgttca ctgaatgcgg cggaatcatc ggtgatcacc
    28021 gggttaaagg ttgggtccat agtgcccatt ttttcctggt gatgcacagg aatatcacac
    28081 cccgggaatg cgccggtaat aaagaactgt gcggtcgcaa cggtacgttg cagactattg
    28141 gcataggcat aaacggtgta cggcggcggg cattcccccg atttcaccat cccctgctct
    28201 gccagccatt cacgcatgta atggcccata tacacttcga gcactccacc tttggtggtg
    28261 agttgcccac cggggacgtc ccattctggc catttattcg gcgtcgactg ctccagcaca
    28321 ctgccattgt tcgccagcgg cgcacgtaag ttatggcggc tcatcatgag cacttgctgt
    28381 agctgatagc cttccggtac ggtttgtgcc tgagcgtttg aagcgagtaa aactatccct
    28441 gccacagctg cggcgattag cgttttgttc attcctggca cctcttttgt tatcaatcac
    28501 taaagagtgt gacagaaata tgactttttt ccgggaacag gttcgcaaaa tggcttgttg
    28561 aacgcctgtt tttctatata catcttcaaa aacaggcaag gtttgacgca aactatgcct
    28621 gattagcccc tgcctcgcgt acgccgctga taattgcacc tgtggctaaa cagaatatct
    28681 gcggcgttac caatacagag acaatattta ccaataaaaa agtgtgagac taataacaat
    28741 ggtttttagc atatgccttt gccattaaat tgcataacga tttaaatagt cgagttgcgc
    28801 attcatttcc ttatttttta acttgtaagc ctgagcaaaa agttcaggag ggaggtaaac
    28861 atcataatga ttgccagtgt ttattaaata accatactca agatcttcat ctgttgaaaa
    28921 ggattcattc cctaaggaga ttgatgccac attactatta acaataaata atgagcaaat
    28981 gctatctgaa agagcagtga ggaaattttt cacctcatta atcctcttct ctgggtcggt
    29041 gtctaaaaca aggcgtgcct tgttatcatt aaatataaac tcgggtaaat gcagtatgca
    29101 ttttttgtca ttcaagcaac tatcaataaa ttatttttta aaaaacagaa agttccatga
    29161 cataagacta ccctccccct gaattttgtt gtcagattgt agatcaacgc caaataacgt
    29221 ctttacatct tccctgagat tatagaatgc cgtatcaaca agagcctcga tcgtttgttt
    29281 atcaacaatt ttaataaact cttcgtatac ttgcttttgc gtcttatggc tgcccagcgc
    29341 ccatgaatac tcatgttctt ttaaaaccag tatggccctt aacagacaat agccatcgcc
    29401 atctactgga gcgtatttta atccataagt tttattaatt tcactctgaa taaaagatat
    29461 accagacaaa tcaataccag aatctaaatc agatattccc ggcgtattta tctgttctgg
    29521 ctggatactc atattttcat catctaaaca atactcagaa aaaacatcat cacacgcagt
    29581 aatgttttca atatattcca taaagccata ccaataataa cctttaaaat ctgtgataga
    29641 ctctgaacct ctgcccggca aggacactct aacaatatca acattttgaa catattgtat
    29701 agtatatgta atacctcctg aaacccctga ataaatttgt gtatcattag tacgcaatgt
    29761 tctcataaat aatgatagat cattatcatt ctgcatttcc tcaaattctt tttctagtga
    29821 ttcacattcc agacatggag tatcctcttc atttacttta cgtctgagtt cattgtataa
    29881 ccgcataaaa cacaccagtg tgttttcaaa aagcgtgatt gaatatgttt gatttttttc
    29941 gtactcattt tttatatcgt tccaggcctg tttcaaataa ttatttgcag tgcaactaat
    30001 tccatgtata ttactaccca tatatagcgt ctataaaatt taataaataa tgacgcccca
    30061 gttaaactta aagtgcctgg ttcaactatc aaaaatcgct cacccttttt cacctgttta
    30121 aaatatgttc agtaacccat cttgatggcg acctcctctc cgcgatgatt tcaataacat
    30181 attctgtgtt ggcatatgaa attttgagga ttaccctaca cttataggag ttaccttaca
    30241 ggggttcctt caatttgtgt tgatttacgc gagataacgc tatggaatta aaggattatt
    30301 acgccatcat gggcgtgaaa ccgacggacg atctcaagac aatcaagacc gcctatcgtc
    30361 gacttgcccg caaataccat cctgatgtca gcaaagaacc ggatgccgaa gcccgcttca
    30421 aagaggtcgc tgaagcctgg gaagtgttaa gtgatgaaca acgtcgcgct gagtatgatc
    30481 agatgtggca acatcgcaac gatccgcaat ttaaccgtca gttccatcat ggcgacggtc
    30541 agagttttaa tgccgaagat tttgacgata tcttctcgtc aattttcggt cagcatgccc
    30601 gccagagccg tcaacgcccc gccgcacgcg gccacgatat tgaaatcgaa gtggcggtat
    30661 tcctcgaaga aacgctgact gagcataagc gtaccatcag ctataacctg ccggtttata
    30721 acgcctttgg catgatcgaa caggaaatcc cgaaaacgct gaatgtgaag atcccggcgg
    30781 gcgtcggcaa tggtcaacgc atccgtctga aaggccaggg gacgccgggc gaaaacggtg
    30841 gtccaaatgg cgatttgtgg ctggtgattc atattgcgcc acatccgctg ttcgatattg
    30901 tcggccagga tctggaaatt gtggtgccgg ttagcccgtg ggaagcggcg ctgggtgcta
    30961 aagtcaccgt tccaacactg aaagaaagca ttttgctgac tatcccgcca ggcagccagg
    31021 ccgggcaacg attgcgcgtt aaaggcaaag gtctggtgag caaaaaacag accggcgatc
    31081 tgtatgcggt actgaaaatc gtgatgccgc cgaaaccgga tgaaaacact gccgcgctgt
    31141 ggcagcaact ggcagacgcc cagtcgtctt ttgatccacg taaagattgg gggaaagcat
    31201 gatggctaat gttacggtga cttttactat taccgaattt tgcctgcata ccggcatctc
    31261 tgaagaggag ttgaatgaaa ttgtcggttt gggggtggtt gaaccgcgtg agatccagga
    31321 aacaacctgg gtatttgacg accatgccgc cattgtggtg caacgcgcgg tacgcctgcg
    31381 tcatgaactg gctctggact ggccggggat cgcggtggcg ctgacgttaa tggatgatat
    31441 tgcacacctg aagcaggaaa accgcctgct acgccagcgg ctttcccggt ttgtagctca
    31501 tccgtgatgt gtgtttgccg gatgtgatga cgcatccggc aactgcgcca taatgcctga
    31561 tgcgacgcta aacgcgtctt ttcaggcgca taaacgacgt tatctgtttt ggtggtcgca
    31621 ctccactaac accagcaata actggcttag tgccgcgtaa aaaccaaagc tgtcatactg
    31681 atggcaacgc gcagcaaact ctggtaacca ttgccacagc gccgtcagtg ttttttgccg
    31741 caaactgtcg attcttcgcg caggaacggt cccctctccc agcgaaaaat gcagatgact
    31801 caacaactcg agatagatcg ccagatggtc tgccggttcg ttgaaattgc cgctggtttc
    31861 catccctgcc tcaactaaca agcgtttaat ctcttgctcg tcctgtttgt aggccgatgc
    31921 atacggcagc gccgcttgtt tgtcggtcat cagaaacagg ccgcaaaagt cagcggccag
    31981 ttccagacgg gcatcgtcac gtactgtcag cgtggcaata cagttttcca gctcattcac
    32041 cgccgcagcc agcggcggtt cgcttttcag caacgaaaac cattcagcca tctgcgcact
    32101 ggcgatttgc gtcagttgtt catcgtccag ttcacgggag aacaactgcg ccagccaggc
    32161 gtaaacacag gcaatctgtt gtgctgtcag cgtggtcatg atttcacctg cgacgcggga
    32221 acatattcgc actgcgccac catctccaca gggccgttaa acgccgtcac ctgctccact
    32281 gctccattgt acttctcaat ttccaccagc gtagtgtgcg cactggtcgc ctgcgccagc
    32341 tgcgaagtac cgatgtcgat ggtcagcacg ttggggttac cgtatttgca cagcgcacca
    32401 ggctcgccgc ctttatctgg atcgtaccat gccccttcgt gaattcgtgc cacgccgggt
    32461 gcatagcggt cagaaactac tgcccctgcc aacacctgac cgcgagcgtt aaagacgcgt
    32521 accacatcac cgttacgaat accgcgcgcg ctggcatcct gcgggttaat gaacactggc
    32581 tctttacccg ctaccgtata ttgctgacgc agagtttccg actcacataa ctgcgagtga
    32641 agtcggaaat ccggatgcac agattgcaga tgcaacggat acttctgcga gccgggccca
    32701 ccgtgggagc gttcgatttt ctcaaaccac atcggatgcc cctgacaatc gtcgtagttc
    32761 atatcggcga tggttttcga gtagatctca atcaggccac tcggcgtgcc cagcggttcg
    32821 agatccggat cttcgcggaa tgcctggtgg cgaacaaaca tctgcggatg gtcaaactcg
    32881 acgtactctt tgttattcca gaagtcatca aacgctggca gatgaacgcc gcgtcctttg
    32941 ccttgttgta caccttcctg ccagatgcgt ttcagccagc ccatttcgtc cagcccttcg
    33001 gtaaaggctt cttcgcgatt aaagcgacgg cacagctcgc ggaaaatatc aaagtcgttg
    33061 cgcgcctcga actgcggcgg caccacctgt ttcatggcga taacgccacg gttggagtgg
    33121 ttgccgtact ggtcgagatc gttacgctca aactgcgtgg tcgcaggcag cacgatatcg
    33181 gcaaagcggc aggttgaggt ccactggtta tctatggcga taaccgtttc cagcttgcgc
    33241 cagccttcaa taatgcggtt gatctgctga tggcgatgga atgggttagt tccggcaaaa
    33301 atacacattt tcagcggcgg cagttttacc gatttaccgt tccagttgat cactttcccc
    33361 ggttcgagga tcgcatcgat aaaacgggca atcggaatgg tgctgctgta gcctttgtaa
    33421 tcactgttgt cgtgaacagg cggaatcgac gtagagccgg agaaaccact cagaataacg
    33481 cctttacgcc ccggcgtgcc tgcgccgtta tagtgccagc caaaaccaaa gccaccacct
    33541 ggcaggccaa tttgccccag catcgccgcc agaaccacaa tcatccacgc ccactgttca
    33601 ccgtgctgca tacgctgcac gcaccagcca gcaataattt gcgttctgtt cgccgccatc
    33661 tgccgcgcca gcccacgaat ggtttcggca tcaatgccgg tcagtttttc agcccatgcg
    33721 gcatctttcg gctgaccgtc tttctcaccc agcaggtacg gcaggaactg ctcaaaaccc
    33781 acacagtagt tagcgaggaa gtttttgtcg tacaggtttt cactgtacag cgtatacgcc
    33841 agcgccagtt gcagcggcac atcagtttgc gggttaaccg caatgtgctt cacatgctcg
    33901 cgccccagat actcatgggt ggatgtgaca accggatcga tgctgatgac ctcaatttca
    33961 ccggcggcga ctttcgcttt tagctgcgcg taatattcat aaacatcgtg atccgggcac
    34021 caccagttcg cttgctggtt tttcagcaaa tcagagcccc acagcacaat ggttttgctg
    34081 ttctgcaata ccagcggcca ggaggtttgc tgttcataca cttccattga gccaaccacg
    34141 cgcggcagga tcacctgcgc agcaccggta gagtaatctc cgcccgtacc aacgctatta
    34201 ccatgcaagg caatagcttt cgccagcatc cccgaagcgt tatggaacat ccccgtcgat
    34261 tgccaaccac tggcggtcag caaggcactc ggcccgtgag ttttctgcac gcgttccagt
    34321 tcttcataga acatgtcgag ggcttcatcc cagctcacgc gcacaaaacg gttatcaccg
    34381 cgctgggagg tatcgctgag atggcgctta cgcagccagt ccacgcgtac catcggataa
    34441 cgaatacgcg ccgcgttgtg tacgtgatcc ggcaatccgg caatcatttt cgacggatat
    34501 ttatccagtt cgaacggttt tgccgccaca aagcgaccat ccttcaccgt cgcgcggata
    34561 gccccccagt gcgacccggt aagaatgccc tctttcgaga tgacagcgtc agtcgccgct
    34621 tgcgccgcag tcgcacggcg cggcgttaac aatgacggcc ccagcatccc ggcgacggtt
    34681 aagccgccga gttgtgccag aaaacgccga cgtgatgcct gaaagagatc gttattgttc
    34741 attatttttc ttccttctta tcgccgtgag ccttacctgc ggtgtcagac gcattcattt
    34801 gcagatattt caacaaggtg cgttcttcac gtttatcgag actggtaaag ccaatcatgc
    34861 cgttgagcgt gccgatccaa ccgttagcgt caaagtgggc gatttccggt gcgccgtggc
    34921 actggttaca ggtgccgttg tacaacgaat ccgcataagc ccagatcggt ttgatatcgt
    34981 tcaccatgtc gcctttcttc atccacgcag tggcctgcaa cttgctccac tcggtattgg
    35041 tgtcggcaac ggtggttttc tccagcgttt ttacctgctg ctgcacatca ccacgaatcg
    35101 aggcaacaaa gatgcgttta cctgggaatt gggtgagtac acgctgacgt ccggcgcttt
    35161 ccgtccagcc ggtaatttca atttgcagcc agtcgccgtc acgtttaagg actttcactt
    35221 ccgaagcagg cagcagagaa ccagaggctt ctttatcgcc tttcgccgca taaattggct
    35281 taatatcaat agagtacagc gtgtcaccac tgtcattagc actggcgcgc agctcatcga
    35341 actgcttacg gaagccgcta ctcatatccg gtaactggtg ggcaatacct ttatgacagt
    35401 cgatgcagga ttgattatct ttcgctgcca ccttcatctg acgtgccgct tcaggatgct
    35461 gcttcgcatg atccatcgca tcgtagttat ggcaggagcg gcaggttgcc gagttgtttt
    35521 ctttcattcg cgcccattca cgctcggcaa gttccgcgcg tttggcttcg aatttttcag
    35581 gtgtatcaat ggagtgagca ataaaggtct ggtagatatc attgctcgct tccagtttgc
    35641 gcttcaccat gcctggaata tccggcggga tatgacagtc atggcattca gctcgcacgc
    35701 cggaggcgtt ctggaaatgc accgactgtt tatattcttc atacaccggt tgcatactgt
    35761 ggcaactgac acaaaattcg gttgtgctgg tgactttgat cccaacgtgt ggcaatacaa
    35821 tcagcgcaat gccaatcaca atcccaattg cgaccagcgc cagtaccgac caacgagcac
    35881 tgggtcggcg tagcgcgttc cagagtttcc gcataatagc ccctgtaaaa ttatggttta
    35941 gtgaagcgat cttaatgagc aaatatgaac agcggcactg gtcaggatga acggcttacg
    36001 gcagaatatg aacagatatg aacagaatga gtaaaaccct ctgatgccac atcacattgt
    36061 tattgttgaa gatgagccgg ttacccaggc gcgattacaa tcttacttca ctcaggaggg
    36121 gtataccgtt tccgttacag cgagcggtgc cgggctgcgg gaaattatgc agaatcagcc
    36181 ggtagattta attctgctgg atatcaactt acccgatgaa aatggcctga tgttaacccg
    36241 cgccctgcga gaacgctcaa cggtggggat tattctggtt accggacgca gcgatcggat
    36301 tgaccgtatt gttgggctgg aaatgggcgc agacgattac gtcaccaaac cgctggaact
    36361 gcgcgaactg gtagtacggg tgaaaaatct gctctggcga atcgacctcg cgcgacaagc
    36421 tcaaccgcac actcaggaca actgctatcg ctttgccggt tattgcctga atgtgtcgcg
    36481 ccatacgctg gagcgggatg gcgagccgat taaactgacc cgcgcagagt atgaaatgtt
    36541 ggtggcattt gtgacgaatc cgggcgaaat tctcagccgt gaacgtctgc tacgtatgct
    36601 ttctgcgcgt cgggtggaaa accctgacct gcgcaccgtc gatgtgttaa ttcgtcgttt
    36661 acgtcataaa ctcagcgcgg atttactggt gacgcaacat ggtgaaggtt atttcttagc
    36721 cgctgatgtg tgctgataaa aatagaccgg acgaaatccc cctggtgaca gcgagcggcg
    36781 aatatgttca cggtcggcat ttttcggcgt cagaactaaa atcggtgggc tgacattatc
    36841 agacaccgat tgcccctgta attgcctgat ggcctgctca accgccagtt ccccctgcca
    36901 gaccatttga tcgctggcag ccataatcac tcttccccgc ttcagcccgc gatacacctg
    36961 atgtgaaaga taaaacgaca ccacggtaag cggcgttttc aggttacgcc cttcacccat
    37021 tgccgcctct gccgcaatgg ccgttccggc aacgacgtcg atttctgggt ggcgttccag
    37081 catctcctgc aacaggttac gctggatttc aatatcgtta tcaccaagcg caatatcaac
    37141 aatacgcacc gggcttccgg caatggctgc gcgaaaaccc tcgaccatct ctttactgcc
    37201 cccggcatta tcgggtccgg gcatcaacag cacgttcagt ggtttaccgt gcgcccattg
    37261 caccaaatat cgcccaggtt gatagcccat ctgaaaccag ggtacaccaa cgcggctttt
    37321 caccagggga tcatcaagag catttaccag ttcgatcacc ggcagatttg ctacctgctt
    37381 ttgcaggtcg ggaaatgacg tagtgctact accgagcaaa atggcctctg cgccccactg
    37441 tttacactgg tcgatttgtg cttgctgggt agccaactgg ctgtagccgc ctgcctccag
    37501 cacttttaaa tccacaccat agcggcgagc tgcctcctgc ataccatagt tcaacgataa
    37561 ccagtatgaa tctttcaggc tgggataaag cgcgcacagt ttccatgcgc gtttggcttt
    37621 aagcggcgta gaggcttgca ccgtgtaatg ctgcgcatca tgccagcgca acaggttatc
    37681 agccgaaaat gccgataaca tgaaaaggga aagaagtaaa aatagcagta cgcgcatgat
    37741 agcctcatca ataataaggc tttatgctag atgcattccg ctttgcgact caaccttttt
    37801 cacctaaagg atgacaaaat aacattaatt acttaaaaat catcgcatta cacgaatctg
    37861 cggttaaata atagactaca aaatgcgaca aaacgcaaca tatccagtca ctatgaatca
    37921 actacttaga tagtattagt gacctgagac agagcattag cgcaaggtga tttttgtctt
    37981 cttgcgctaa ttttttgtca actcactggg ttagttgaat ttacaaccat gctcagtatc
    38041 tggataagcg cagagaaatg atgcagtggt gggcggactg gattgatgaa aaggtggagt
    38101 gatccacctt agctatcgaa tgacacaaag ccttgtaatc cagtgcaaag ctttgtttgt
    38161 ctcagttttg tcctgtgtgc cagatattga atggcctacg cctccggcag ttcaggccag
    38221 atgacatccg gcgcggtgct ggtatctgtt gcttccaccg catcaatgta atccagcgca
    38281 gcgttaagtc tggcggtttc tgcctgcgtc agattccgtc cggcccgcaa tttcagctga
    38341 atcagactaa tggaagccat tgcagtatca atcagtgact ggcgctgtgc ttctgccgct
    38401 tctactgcgg cgctatgctg tgcctcagta tccgtcaccc atttctcacc atcccattta
    38461 tcatatggcg ttaatggggc gataatggtt gtattttcag ggtaatcccc cggagctgtg
    38521 atttctttgg cgtctcccgt ttcggtgtta tagacgattt ctccgcgatg gtctggcaca
    38581 tattcccatg agtttaaatc tatcgaacgg cagatagca
//
LOCUS       DAHBHC010000045        37924 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHBHC010000045 DAHBHC010000000
VERSION     DAHBHC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.045"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..671
                     /gene="yiaJ"
                     /locus_tag="M2A35_002741"
     CDS             <1..671
                     /gene="yiaJ"
                     /locus_tag="M2A35_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCB7199024.1"
                     /translation="RLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIHIA
                     APHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKI
                     YMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGV
                     SCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRDDQ
                     GAIT"
     gene            773..1246
                     /locus_tag="M2A35_002742"
     CDS             773..1246
                     /locus_tag="M2A35_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCB7199025.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1398..2651)
                     /gene="avtA"
                     /locus_tag="M2A35_002743"
     CDS             complement(1398..2651)
                     /gene="avtA"
                     /locus_tag="M2A35_002743"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCB7199026.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(2829..4859)
                     /gene="malS"
                     /locus_tag="M2A35_002744"
     CDS             complement(2829..4859)
                     /gene="malS"
                     /locus_tag="M2A35_002744"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCB7199027.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQSTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            5179..6003
                     /locus_tag="M2A35_002745"
     CDS             5179..6003
                     /locus_tag="M2A35_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCB7199028.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(6111..7289)
                     /gene="xylR"
                     /locus_tag="M2A35_002746"
     CDS             complement(6111..7289)
                     /gene="xylR"
                     /locus_tag="M2A35_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCB7199029.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7367..8548)
                     /gene="xylH"
                     /locus_tag="M2A35_002747"
     CDS             complement(7367..8548)
                     /gene="xylH"
                     /locus_tag="M2A35_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCB7199030.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8526..10067)
                     /gene="xylG"
                     /locus_tag="M2A35_002748"
     CDS             complement(8526..10067)
                     /gene="xylG"
                     /locus_tag="M2A35_002748"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCB7199031.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10145..11137)
                     /gene="xylF"
                     /locus_tag="M2A35_002749"
     CDS             complement(10145..11137)
                     /gene="xylF"
                     /locus_tag="M2A35_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7199032.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLINVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            11503..12825
                     /gene="xylA"
                     /locus_tag="M2A35_002750"
     CDS             11503..12825
                     /gene="xylA"
                     /locus_tag="M2A35_002750"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCB7199033.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            12897..14351
                     /gene="xylB"
                     /locus_tag="M2A35_002751"
     CDS             12897..14351
                     /gene="xylB"
                     /locus_tag="M2A35_002751"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCB7199034.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            14520..14861
                     /locus_tag="M2A35_002752"
     CDS             14520..14861
                     /locus_tag="M2A35_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199035.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            14907..15344
                     /locus_tag="M2A35_002753"
     CDS             14907..15344
                     /locus_tag="M2A35_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCB7199036.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(15386..16381)
                     /gene="wecH"
                     /locus_tag="M2A35_002754"
     CDS             complement(15386..16381)
                     /gene="wecH"
                     /locus_tag="M2A35_002754"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCB7199037.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16556..16855
                     /locus_tag="M2A35_002755"
     CDS             16556..16855
                     /locus_tag="M2A35_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCB7199038.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            16950..17861
                     /gene="glyQ"
                     /locus_tag="M2A35_002756"
     CDS             16950..17861
                     /gene="glyQ"
                     /locus_tag="M2A35_002756"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCB7199039.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            17871..19940
                     /gene="glyS"
                     /locus_tag="M2A35_002757"
     CDS             17871..19940
                     /gene="glyS"
                     /locus_tag="M2A35_002757"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCB7199040.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            20266..20418
                     /gene="hokA"
                     /locus_tag="M2A35_002758"
     CDS             20266..20418
                     /gene="hokA"
                     /locus_tag="M2A35_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCB7199041.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(20606..20818)
                     /gene="cspA"
                     /locus_tag="M2A35_002759"
     CDS             complement(20606..20818)
                     /gene="cspA"
                     /locus_tag="M2A35_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCB7199042.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(21099..21389)
                     /locus_tag="M2A35_002760"
     CDS             complement(21099..21389)
                     /locus_tag="M2A35_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCB7199043.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            21823..22533
                     /locus_tag="M2A35_002761"
     CDS             21823..22533
                     /locus_tag="M2A35_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCB7199044.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(22583..23557)
                     /gene="ghrB"
                     /locus_tag="M2A35_002762"
     CDS             complement(22583..23557)
                     /gene="ghrB"
                     /locus_tag="M2A35_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCB7199045.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(23661..24320)
                     /locus_tag="M2A35_002763"
     CDS             complement(23661..24320)
                     /locus_tag="M2A35_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCB7199046.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            24473..26806
                     /gene="bisC"
                     /locus_tag="M2A35_002764"
     CDS             24473..26806
                     /gene="bisC"
                     /locus_tag="M2A35_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCB7199047.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGFSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PRSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(26775..27215)
                     /locus_tag="M2A35_002765"
     CDS             complement(26775..27215)
                     /locus_tag="M2A35_002765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7199048.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(27212..27775)
                     /gene="tag"
                     /locus_tag="M2A35_002766"
     CDS             complement(27212..27775)
                     /gene="tag"
                     /locus_tag="M2A35_002766"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCB7199049.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            27933..28631
                     /locus_tag="M2A35_002767"
     CDS             27933..28631
                     /locus_tag="M2A35_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7199050.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            28860..30062
                     /locus_tag="M2A35_002768"
     CDS             28860..30062
                     /locus_tag="M2A35_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199051.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
     gene            30387..30911
                     /locus_tag="M2A35_002769"
     CDS             30387..30911
                     /locus_tag="M2A35_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HCB7199052.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            30995..31681
                     /gene="lpfB"
                     /locus_tag="M2A35_002770"
     CDS             30995..31681
                     /gene="lpfB"
                     /locus_tag="M2A35_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HCB7199053.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNSNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            31705..34236
                     /gene="lpfC"
                     /locus_tag="M2A35_002771"
     CDS             31705..34236
                     /gene="lpfC"
                     /locus_tag="M2A35_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HCB7199054.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     NRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            34247..35302
                     /gene="lpfD"
                     /locus_tag="M2A35_002772"
     CDS             34247..35302
                     /gene="lpfD"
                     /locus_tag="M2A35_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HCB7199055.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            35308..35832
                     /gene="lpfE"
                     /locus_tag="M2A35_002773"
     CDS             35308..35832
                     /gene="lpfE"
                     /locus_tag="M2A35_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCB7199056.1"
                     /translation="MKRTILSLCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            36085..37776
                     /gene="eptB"
                     /locus_tag="M2A35_002774"
     CDS             36085..37776
                     /gene="eptB"
                     /locus_tag="M2A35_002774"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCB7199057.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLIFIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
BASE COUNT         9443 a         9130 c         9624 g         9727 t
ORIGIN      
        1 atcgcttatt gcagggatta cagtcctgcg gctatgtgac caccgcgccc gccgcaggga
       61 gttaccgcct gactaccaaa tttattgccg tcgggcagaa ggcgctgtct tcgctgaata
      121 tcattcatat cgccgctccg catcttgagg cactgaacat cgccactggt gaaaccatta
      181 acttctccag ccgcgaagac gatcacgcta ttttgattta taagctggaa cccacaaccg
      241 ggatgctgcg aacccgtgcc tatattggcc agcatatgcc gctctactgt tccgcaatgg
      301 gcaagatcta catggcgttt ggtcacccgg actacgtgaa gtcatactgg gaaaaccatc
      361 agcatgagat ccagccgtta acccgcaata ccattaccga gctgcccgcg atgttcgacg
      421 aactggcgca cattcgtgaa agcggagcgg cgatggacag agaagaaaac gaactcggcg
      481 tctcctgtat tgctgttccg gtgtttaata ttcatgggcg ggtgccgtac gccgtgtcga
      541 tttcgctttc gacatcacgt ctgaaacagg tgggggagaa aaatctcctg aaaccactgc
      601 gtgaaaccgc gcaggctatt tctaatgaac tgggatttac tgtcagggac gaccagggcg
      661 cgatcacata acgctttgga caagtgccaa aactttaaca tttccttcgt tggatcaaag
      721 cagtagggac gcgctctctg gcactctgct gttttagtgc aaaggagtga tcatgaaccg
      781 gtttattatt gcggatgcga cgaaatgtat cggttgccgt acctgtgaag tggcttgcgc
      841 agtgtcgcat caggagaatc aggattgcgc tgcgttgtca ccagacgagt ttatttcccg
      901 tattcgtgtc attaaagacc acagctggac cacggcagta gcctgtcatc agtgtgaaga
      961 tgcaccgtgc gcgaatgtct gccctgttaa cgcgataagc cgcgaacatg ggcatatttt
     1021 cgttgaacaa acacgttgca ttggctgtaa aagctgtatg ctggcttgcc cgtttggtgc
     1081 gatggaggtc gtttcttcgc gcaaaaaggc gagggcgatt aagtgcgatc tgtgctggca
     1141 tcgggagacg gggccggcct gtgttgaagc ctgcccgaca aaggcgttgc agtgcatgga
     1201 tgtcgagaaa gtgcagcggc atcggctacg gcagcagcct gtttgaaaca tttttccggc
     1261 gtttctcaac gccggaatag tgcgctttgt ttatgccaga tgcggcgtaa acgccttatc
     1321 cggcccacaa attcttgcaa attcaatata ttgcagaaat catgtaggcc taataagcgt
     1381 agcgcatcag gcaattttta gtgactttca gcccaggctc tttctatctc ttccgccaga
     1441 atcttcaccc ccgcctcaat tttctccggc tctggtacgt agttcatgcg catacattga
     1501 tgcgtatgcg gccacggttt gtccagccct gggaagaagt tgtgccccgg caccatcagc
     1561 acgccgcgtg ctttcaggcg ctgatagagc tgctcggtcg taatgggcaa atccttaaac
     1621 caaagccaga ggaaaatggc tccttctggt ttatgaatta ggcagcgatc ttccggtaaa
     1681 tagcggcgaa tgatggcgat agtttcctga acacgctggt agtaaaacgg tttgatgact
     1741 gtttcagaca ggcgcagcag atcgttacgc ttaatcattt cacacatcat cgccggacca
     1801 ataccgccag gtgccaggct gataatgccg ttcatattgg tgatggcagt gatgattttt
     1861 tcattggcga tgataatgcc gcagcgggag ccaggcagcc ccagcttgga aagactcatg
     1921 cacagcacga tattcggatt ccatagcggg cgcgcttcac tgaagatgat acccgggaac
     1981 gggacgccat aagcgttatc aatcaccagc ggaatgccgt gttgattcgc cagcgcgtca
     2041 agcttcagca actcttcgtc agtaatcaca ttgcctgttg gattcgtcgg ccgggagacg
     2101 caaatcatcc cggtttcttc gccaatatgc agatgctcaa aatcgacgtg gtatttaaac
     2161 tggccttccg gcagcagttc aatattcgga cgcgcagaga caaacagatc ttcttccagt
     2221 ccggcgtcag cgtagccaat gtattccggt gccagcggga acagcacttt tttgacccga
     2281 ccatcggcac ggcgtccggc aaacagatta aataagtaga aaaacgcgct ctggctgccg
     2341 tttgttagtg caatattctg tgcttcgata tcccaaccca acttctcgcg cagcattccg
     2401 gcaagcagtg tgagtagctc cgttttcccc tgtggaccgt cgtaattgca cagtgcatca
     2461 gtcgctttgc cactttccag catgtcggtc agtagcgtct ggaagtagtc ctgcatttcc
     2521 gggatctgcg ccggattacc gccgccgagc ataatcgcgc caggcgtgcg taaaccgtcg
     2581 ttcagatctt ccatcagcag cgtaatgccg gagtggcggg taaatttgtc accaaaaagg
     2641 gagaatgtca tagcgggata tctgtcgaac tctgtagcaa ggaaggtaac aataacgcta
     2701 catttgctgg ggtgcaaatc ggtctgttgg agagtgagcg gtgctttatc ctgcaacgct
     2761 gattagggct gacattttat cctggttggc ggcttcccga ggtactgacg aacgggaagc
     2821 cggaaaagtc actgttgccc agcccagatg accagcactt tatcgtcgcc atgctcacga
     2881 acaaagccgt agccctgctt cagcgaaagt gtcgtttgtt tgcccgcgcc aattgcggga
     2941 tggcgggcgc ggaactggct gattttctgc cagtgcgcga cgttggcggc agatttaccg
     3001 ctaacatcct gccagttcat atccgaacgt gtactttgca gcggatcaga acctgtagga
     3061 ccgaacggac gcgaggattc atcgccataa aagatttgta ccgcgcctgg cgctaatagt
     3121 aataactctg ctgctttgtc gcccccttca cggaacagac gggtatcatg cgacgagagg
     3181 tagctcaaca cgttgaaacc ctgcaatttc tccgccattt gctgccaggt cgtatccatc
     3241 tgcgccagac aatcgactgc tttcgccgcc tgctcctgat aatcgaaatt gatcatcgca
     3301 tcgaagccgt ggcgatagta gtcactttgc atcacgccgt ggccccaggc ttcaccggtc
     3361 atccagaaag gtttgtcatc taatgctttg tcggggttag cttttttcca ttcgcgaagc
     3421 gcggcgctgg cttcggtttt cagttgctgc caggcgggca actcaacatg tttggcggta
     3481 tcgacccgaa aaccatcaat cccatagtcg cggacccact gacttaacca gtgggttaag
     3541 taatcgcgag gtgtaaagcc ttcgatgact ttagcgtgag tatccgtttt gtttttatag
     3601 aacaccggca gaccagaagc ggtagttgat tcggttttga tatccggcaa aaaggccagc
     3661 gacatagtga gatcgtcgaa tccaggattg tcgtaatcgc cgatatccgt tctgatccag
     3721 ttttttcccc accatttatc ccagcctgtt ttgtcgctga aattaatgta atcgttaaag
     3781 ctatgccagg tttgcccggc ggcaggtttc cagtcgctcc agcgttcacc cagcgttttt
     3841 ttcacctcgt caccagaaag atataacgcg ccaaactgat actcctgcat atccgccagc
     3901 gtggcatagc cggtgtggtt catcacgaca tcaaagagaa tacgaatacc gcgctgatgt
     3961 gcgctatcaa ccagcgtccg tagatcggct tcgttgccca tattggcatc aagattcgtc
     4021 cagtcctgtg tgtaataacc gtggtaggca taatgcggga aatcgccttt tgtaccgccg
     4081 ccgacccagc cgtgaatttg ctcaaatggg gcgcttatcc ataaagcatt aacgcccaac
     4141 tgctggaggt aatccagttt gttggtcagg ccgcgtaaat cgccgccgtg aaaagtgcca
     4201 atttccgcca taccgtcttt atgacgtccg taactctggt cattactggg atcgccgttt
     4261 tcgaaacgat ctgtcagcac aaagtaaacc gtggcgttat gccagtcgaa aggggcaggg
     4321 gcgtcagttt ctgcccgttc cagcaggagt aaaccgttgc tggtagcagc gggttgtaac
     4381 gttatttgac cgttcttcac tatcgcaatt tgctggctgt aataatcccg tacggcggct
     4441 ccttccggga aagtggcgct gacatccagc gtgagcggta atccatccca tttcgggcat
     4501 tcacggacca ggtttgctac cggttcggcg gcgttctgga tggaaatcat caatgttggc
     4561 gtaccggagc gggtgtctat ttgcagcgta tatttgccgt ctctgaacaa tcgccattga
     4621 ggcggcgtgt tgctacaagg ttgcagggaa agcatctgat tgagttttat cgcatctgca
     4681 ggctgccagc actgttggtc aaaatttagc gtgagtggac gcgtaccttt gggcaactgc
     4741 gcgtggctga caaatgttcc cgttccctgt tcgctaaagg cagggaaccc cggagaagtc
     4801 cagctggcgg caacggcgaa gccaggaagg agtgtcagaa aacaggcggc gagtttcatg
     4861 gtggatgagt ccttattgct gcgatttcag acagtttgcc agcgataagc cagacaaaac
     4921 tcatccctga gcggtaagtc cacaggatga gaagcaaggg tgagcgatct cgcgcaaaaa
     4981 acggctgaat tttgcgataa accccacatt ttctgcgatt tagcgccaat ctgaatcgtt
     5041 aacacgtgac atagtttcag atttggacta ttccttgggg tgctcttgac agctattttt
     5101 gatatgattt gagattcaac tctcaaattt gtgaaaaata taaggtgttg gaatgattaa
     5161 atccgaccag gagacctgat gattttgact cccatacgac gatatggggc gatgattctt
     5221 atgttactca ctctggtgtt ttcgagtgag gtgttagcga agacgcacac aacaacagcg
     5281 agtcaaaagt cccacttaac taaagctagt aataaacagg taagcagtaa acaagagtat
     5341 tctcgcaata gtgcaaagag tagttcactt cctgatttgc gaaaataccc ttccggaaca
     5401 ccaaggaaaa aggcgtttct ccggaccgta atgccttaca ttaccagcca aaatgcagcc
     5461 attactgcag aacgtaactg gctgatttca aaacagtatc agggccaatg gtcacctgct
     5521 gagcgtgcgc gtctgaaaga catcgccaaa cgctacaagg tgaagtggtc tggtaatacg
     5581 cgaaaaatcc cgtggaatac cttgcttgaa cgcgtagaca ttatccctac cagtatggtg
     5641 gcgacgatgg ctgcagcaga aagcggttgg ggaacgtcga agctggcgcg caacaataac
     5701 aatctgttcg gcatgaaatg catgaaagga cgttgtacca atgcgccggg taaagtgaaa
     5761 gggtactcac agtttagttc tgtcaaagaa tcggtgagcg cctatgtcac taacctgaat
     5821 acgcacccgg cttactcttc gttccgtaaa tcgcgtgcgc agctgcgtaa agcggatcag
     5881 gaagtgactg ccacagcgat gattcacaag ctgaagggct actcgaccaa agggaagagt
     5941 tataataact acctgttcgc aatgtaccag gataaccaac ggttaatcgc ggcgcatatg
     6001 taattgcatt tccttgagcc ttatctgatt tgtcggtcgg ataaggcttt tgctcgcatc
     6061 aggtggctgt gctgagttcc ctgatgtgac gccgacaatt ctcatcatcg ctacaacatg
     6121 acctcgctat ttacatcgcg atactctttt ggcgttgtgt catatgcttt tttaaaaaca
     6181 gagtagaaat attgcagcga tggataaccg cacatttgcg atatctcatt gatcgacaag
     6241 gtggttgaaa tcagcagact gcgcgctttc tccagcttct cggcatgaat catggcatgg
     6301 atggtttcac ccacctcttc tttaaaacgc ttctcaagat tggagcgcga gatcccgacc
     6361 gcatccagta cctgatccac tttaatccct ttacaggcgt gattacgaat gtaatgcatg
     6421 gcctgaataa cggcgggatc ggtcagcgag cgataatctg ttgagcgccg ttcaatgacg
     6481 cgaactggtg ggaccaaaat tcgctgtagc ggcatttctt ctttatctaa taatcgatgc
     6541 aacagttttg ccgcctgata gcccatttgc cgcgcgccct gagcgaccga agaaagggcg
     6601 acacgcgaca gatagcgggt cagttcttcg ttatcgatgc caatcacgca taatttttcc
     6661 ggtacgggaa tatgtagatg ttcacatact tgcagaatat gccgcgcccg ggcgtcagta
     6721 acggcaataa tcccggtttg cggtggcagc gtttgtagcc agtctgccag ccgattttgc
     6781 gcatgttgcc agttctctgg cgcggtttct aacccctgat aaaccactcc gcgatacttt
     6841 tcttcggcga caagctgacg aaatgcatat tcgcgttcag tggcccaacg tttgccgctt
     6901 gattccggaa gaccataaaa agcaaagcgg ttaacgcctt tctcttttaa atgcaaaaat
     6961 gcgctttcaa ccagcgcata gttatcggtg gcaatgtaat gaacgggtgg gtaactttct
     7021 gcaaggtgat acgagccgcc aaccccaaca atggggacgt cgacatcagc cagcgcttgc
     7081 tcgatctgtt tgtcgtcgaa gtcggcaatg acgccatctc ctaaccagtc cttgatttta
     7141 tcaatgcggg cgcggaaatc ttcttcaatg aaaatatccc attctgattg tgatgcctgt
     7201 aaatattccc ctacgccttc tactacctgc cggtcatagg ctttattggc attgaacagt
     7261 aatgtgatgc ggtgacgttt agtaaacatg gttcttttcc tgctgaatca tgcaaaaact
     7321 caaaaccggt aatacgtaac cggctttgag aaaattttta tcaaaatcaa gaacggcgtt
     7381 tggttgcgga gtccatccat actgccagca acagaatcgc acctttaacg atatactgcc
     7441 agaaggtcgg tacatccatc atactcatgc cgttatccag tgaagccatg ataaatgccc
     7501 ccattactgc tccggcaacg cttcccacac cgccagccag gctggtgccg ccaatcacgc
     7561 atgctgcaat tgcgtccagt tcggcgatat ttcccgcaga aggtgaacca gcgccaagtc
     7621 gagaactaag gattaatccg gcgatggcta ccattaatcc gttaatcgcg aacacggcaa
     7681 gtttggtgcg ttcaacgtta atcccggaga gacgtgctgc ttccagattg ccgccgatgg
     7741 cataaatgcg tcgtccaaat gccgtccgcg ttgccataaa cattccgccg agtaacagca
     7801 acgtcagcag cagaacagga gtgggaacgc cacggtaatc attcaacagc cagattgcgc
     7861 ctaatacgat gatagcggtt aaagcctggc gaccgactac tgcggtagag gccggagact
     7921 gcaaacccaa agcctgacgg cgcattcttc cgcgccattg ccaaccaaca aaagccatta
     7981 agccaagcgc gccaatgatg aagccagtgc tggcaggtag atagctttgc ccaatttgtg
     8041 acatcgcggc gctggtgggg gaaacagtcg tgccgttggt gatgccaatg agtatgccgc
     8101 gaaatgccaa catgcccgcg agggtgacaa taaatgaagg gactttgcgg tacgcgaccc
     8161 accatccgtt ccaggcaccg agaagcagtc ccagaaccaa cgtcacaatg atggtaagtg
     8221 gcaaaggcca gcctaaccag acgtcacaaa tcgccgcgac gccacctaat agccccatca
     8281 ttgagccgac ggaaaggtcg atttcagcag aaattatgac gaacaccatt cctaccgcga
     8341 ggatgccggt aatcgcggtc tggcgtaaca ggttggagac gttacgggcg cttaagtagg
     8401 caccatcggt ggtccaggta aagaacagca tgattgcgat gatagctgca atcatcacga
     8461 agacctgcaa attcagtgat ttcagcccgg aaaagccacc ggatgtcggt acggccaatt
     8521 tcacttcaga cggattgctt ttcgacatga tgttcgctcc tcaatgcggc ttccatcacc
     8581 tgctcctgag tcaggttatg atttatcagg ttggctttta gtttcccttc atgcatcacc
     8641 agtacccgat cgctaaggcc gagcacttca ggtaattcgg aagagatgac aataacggca
     8701 ataccctgct ggacgagttg gttaattaat ttgtagatct cgtatttcgc gccaatatcg
     8761 atacccctgg taggttcatc aagaatgagg atgcgcgggt taagtaacag acagcgagcg
     8821 aggatcgctt tttgctgatt gccgccgctc aaacgtccaa tagcaaggtc gggggacgac
     8881 gttttaactt tgagttgctg aattgattcc agaatacatt tttgctctgc cgcgtcatca
     8941 agctggctaa tgccaccggt aaatttattg agtgcggcga gggtaatatt tttaccaacc
     9001 gccattaccg gaacgatgcc gtcgcgcttt ctgtcttcgg gtaccatcgc aatcccctgg
     9061 gcgatggctt gctgacagtt acgaatatct acctgtttgc catcaatata aatttttcct
     9121 tcccattgtc cgggccacac gccaaacagg cactgaatgg tctcggtacg tccggcacca
     9181 acgagtccgg caatacccag tatttcgcca cgtttcaggg aaaacgagac atcattaact
     9241 cgtttaatat gacgattgac cggatgccat gccgtcagat gttcaatacg taatatttca
     9301 tctccggtgg tatgtggttc attagggtaa agcgcggtta actctcgccc gaccatcatg
     9361 gtgataatat cgtcttcact cattccggca gcatcacgcg taccaatgtg ctgtccgtcg
     9421 cgaataacgc aaatcgtatc ggaaatcgct ttgacttcgt tgagtttgtg cgaaatataa
     9481 atacaggcga taccgtgctg ttgtagatcg cgaataatat ccagtaaaac cgacgtttcc
     9541 tgctcagtta atgaggctgt cggttcatcg agaattaaca agcgcacctg tttattaagt
     9601 gccttggcaa tttcaaccag ttgttgttgc ccaagtccta aatcgccaac gcgggtatca
     9661 ggtgaaatgg ataaactgac ctgtgcgagc agcttctgac agcgtagcgt catcaggtca
     9721 taatccataa tgccattgtg ggttatttcg ttacccagga agatattttc cagcacggtc
     9781 aattctttca ccagggccaa ttcctggtga atgatggcga tacctttgcg ttcggtatcg
     9841 cggatgtgac tcgcctgaat ctcttctccc gcaaaaataa tttcgccttc gtaggagcca
     9901 tggggataaa taccacacag cactttcatc agtgttgatt taccagaccc attttcccca
     9961 caaagtgaga cgatttcgcc agcattcaac cgcaagcaga cgttatcaat cgccttcaca
    10021 ctgccgaagg ttttggtaat attcttcatt tcaagtagat aaggcataac gactccacct
    10081 aagccaattc attcacgcgg catggagaga aatcacgccc ccgctccgcg ccggggcgta
    10141 acgcttacag ctcgctctct ttgtggaatc cgtctttaac taccgtgtct ttgatgttgt
    10201 ttttattcac atcgatcggt gtcaggaggc gggagggcac atctttcagg ccattattca
    10261 gtgtggtatc tgcttttggt tcctgaccat tgcccaactc aacggcaatt tctgcggcag
    10321 tatttgccaa caacgtaata ggtttataca ccgtcatagt ttgcgtaccg gcagcaatac
    10381 gtttaatacc tgcgagatcc gcatcctggc cggaaattgc tactttccct gataaacctt
    10441 gcgcgcttaa tgcctgaatt gccccacctg cggtggcatc gtttgaggca actacagcat
    10501 caattttgtt attattggcg gttagcgcgt tttccataat tttcaatgcg ttttccggta
    10561 accagccatc aacccattgg tcaccaacga ctttaatttt tccggaatca acgtaaggtt
    10621 ttaacacttt catttgtccg gcgcggaaca gcttggcgtt gttatctacc ggcgagccgc
    10681 ccatcaggaa gtaattacct tgcggaacaa tatcgaccag ggcttttgcc tgcagttcac
    10741 cgactttttc gttatcgaaa gaaatataaa aatcgatatc cgcatcgtta atcatacgat
    10801 cgtaagctaa tactttaata ccttcttgtt tggcttcttt tacaacgtta attaatacct
    10861 gaccgttata cggaataatg acaagaacat cgacaccccg gtttatcatg ttttcaatct
    10921 gcgacatttg tgtttcttca ttgccatttg cagactgtac aaatactttc gcgccgagag
    10981 attctgcctt tttcacaaaa atatctcgat ctttttgcca gcgttcaaga cggagatcat
    11041 caatcgccat acctattttg acttctttgg cgtgtgccgc aacgttggta agcagaagtg
    11101 aggtgcaaag ggtgagtaga atgttcttta ttttcatggt gtagggcctt ctgtagttag
    11161 aggacagctt taataagtaa caatcaccgc gataaacgta accaattttt agcaactaaa
    11221 caggggaaaa caattacaga tttttatctt tcgattacga tttttggttt atttcttgat
    11281 ttatgaccga gatcttactt ttgttgcgca attgtactta ttgcattttt ctcttcgagg
    11341 aattacccag tttcatcatt ccattttatt ttgcgagcga gcgcacactt gtgaattatc
    11401 tcaatagcag tgtgaaataa cataattgag caactgaaag ggagtgccca atattacgac
    11461 atcatccatc acccgcggca ttacctgatt atggagttca atatgcaagc ctattttgac
    11521 cagctcgatc gcgttcgtta tgaaggctca aaatcctcaa acccgttagc attccgtcac
    11581 tacaatcccg acgaactggt gttgggcaag cgtatggaag agcacttgcg ttttgccgcc
    11641 tgctactggc acacattctg ctggaacggg gcggacatgt ttggtgtggg ggcgtttaat
    11701 cgtccgtggc agcagcctgg tgaggcactg gcgttggcga agcgtaaagc agatgtcgca
    11761 tttgagtttt tccacaagtt acatgtgcca ttttattgct tccacgatgt ggatgtttcc
    11821 cctgagggcg cgtcgttaaa agagtacatc aataattttg cgcaaatggt tgatgtcctg
    11881 gcaggcaagc aagaagagag cggcgtgaag ctgctgtggg gaaccgctaa ctgctttaca
    11941 aaccctcgct atggcgcggg tgcggcgacg aacccagatc ctgaagtctt cagttgggcg
    12001 gcaacgcaag ttgttacagc gatggaagca acccataaat tgggcggtga aaactatgtc
    12061 ctgtggggcg gtcgtgaagg ttacgaaacg ctgttaaata ccgacttgcg tcaggagcgt
    12121 gaacagctgg gccgctttat gcagatggtg gttgagcata aacataaaat cggtttccag
    12181 ggcacgttgc ttatcgaacc gaaaccgcaa gaaccgacca aacatcaata tgattacgat
    12241 gccgcgacgg tctatggctt cctgaaacag tttggtctgg aaaaagagat taaactgaac
    12301 attgaagcta accacgcgac gctggcaggt cactctttcc atcatgaaat tgccaccgcc
    12361 attgcgcttg gcctgttcgg ttctgtcgac gccaaccgtg gcgatgcgca actgggctgg
    12421 gacaccgacc agttcccgaa cagtgtggaa gagaatgcgc tggtgatgta tgaaattctc
    12481 aaagcaggcg gtttcaccac cggtggtctg aacttcgatg ccaaagtacg tcgtcaaagt
    12541 actgataaat atgatctgtt ttacggtcat atcggcgcga tggatacgat ggcactggcg
    12601 ctgaaaattg cagcgcgcat gattgaagat ggcgagctgg ataaacgcat cgcgcagcgt
    12661 tattccggct ggaatagcga attgggccag caaatcctga aaggccaaat gtcactggca
    12721 gatttagcca aatatgctca ggaacataat ttgtctccgg tgcatcagag tggtcgccag
    12781 gagcaactgg aaaatctggt aaatcattat ctgttcgaca aataacggct aactgtgcag
    12841 tccgttggcc cggttatcgg tagcgatacc gggcattttt ttaaggaacg atcgatatgt
    12901 atatcgggat agatcttggc acctcgggcg taaaagttat tttgctcaac gagcagggtg
    12961 aggtggttgc ttcgcaaacg gaaaagctga ccgtttcgcg cccgcatcca ctctggtcgg
    13021 aacaagaccc ggaacagtgg tggcaggcaa ctgatcgcgc aatgaaagct ctgggcgatc
    13081 agcattctct gcaggacgtt aaagcattgg gtattgccgg ccagatgcat ggagcaacct
    13141 tactggatgc tcaacaacgg gtattgcgcc ctgccatttt gtggaacgac gggcgctgtg
    13201 cgcaagagtg cactttgctg gaagcgagag ttccgcaatc acgagtgatt accggcaacc
    13261 tgatgatgcc cggatttact gcgcctaaat tgctatgggt tcagcggcat gagccggaga
    13321 tattccgtca aatcgacaaa gtattattac cgaaagatta cttgcgtctg cgtatgacgg
    13381 gggagtttgc cagcgatatg tctgacgcag ctggcaccat gtggctggat gtcgcaaagc
    13441 gtgactggag tgacgtcatg ctgcaggctt gcgacttatc tcgtgaccag atgcccgcat
    13501 tatatgaagg cagcgaaatt actggtgctt tgttacctga agttgcgaaa gcgtggggta
    13561 tggcgacggt gccagttgtc gcaggcggtg gcgacaatgc agctggtgca gttggtgtgg
    13621 gaatggttga tgctaatcag gcaatgttat cgctggggac gtcgggggtc tattttgctg
    13681 tcagcgaagg gttcttaagc aagccagaaa gcgctgtaca cagcttttgc catgcgctac
    13741 cgcagcgttg gcatttaatg tctgtaatgc tgagtgcagc atcgtgtctg gattgggtcg
    13801 cgaaattaac cggcctgtgc aatgtcccag ctttaatcgc tgcagctcaa caggctgatg
    13861 aaagtgccga gccagtttgg tttctgcctt atctttccgg cgagcgtaca ccacacaata
    13921 atccccaggc gaagggggtt ttctttggtt tgactcatca acatggcccc aatgaactgg
    13981 cgcgagcagt gctggaaggc gtgggttatg cgctggcaga tggcatggat gtcgtgcatg
    14041 cctgcggcat taaaccgcaa agtgttacgt tgattggtgg cggggcgcgt agtgagtact
    14101 ggcgtcagat gctggcggat atcagcggtc agcagctcga ttaccgtacg gggggggatg
    14161 tggggccagc actgggcgca gcaaggctgg cgcagattgc ggcgaatcca gagaaatcgc
    14221 tcattgagtt gttgccgcag ctaccgttag aacagtcgca tctaccagat gcgcagcgtt
    14281 atgccgctta tcagccacga cgtgaaacgt tccgtcgcct ctatcagcaa cttctgccat
    14341 taatggcgta aacgttatcc cctgcctgac cgggtggggg ataattcaca tctatatatc
    14401 tcactaatta attaatattt agtatgaatt tattctgaaa atcatttgtt aatggcattt
    14461 ttcagttttg tctttcgttg gttactcgta atgtatcgct ggtagatatg gagatcgtta
    14521 tgaaaacctc aaaaactgtg gcaaaactat tatttgttgt cggggcgctg gtttatctgg
    14581 ttgggctatg gatctcatgc ccattgctaa gtggaaaagg ctattttctt ggcgtgttaa
    14641 tgacagcaac ttttggtaac tatgcatatc ttcgcgcaga aaaactcggg caactggata
    14701 atttttttac ccatatctgc cagttagttg cgttaatcac tatcggtctc ttgtttatcg
    14761 gtgttttaaa tgcacctatc aatgcttatg aaatggtgat ctaccccatc gccttttttg
    14821 tctgcttgtt tggtcaaatg cgtttgtttc gctcggtatg agcaacataa agctcttaca
    14881 cattcaggaa tgaaaggaat actgtgatgg acaacaaaat atcaacctat tcaccggcct
    14941 ttagtattgt gtcatggata gctctcgttg gtggtatcgt tacctatctg ttagggctat
    15001 ggaatgcaga gatgcagtta aatgaaaaag ggtattattt tgccgtactg gtgttaggac
    15061 tgttttctgc tgcgtcttat caaaagaccg ttcgggacaa gtatgaaggc ataccgacca
    15121 cttccattta ttatatgacc tgcctgactg tctttattat ctctgttgca ttactgatgg
    15181 taggtctgtg gaatgcgaca ttattactca gtgaaaaagg tttttatgga ctggctttct
    15241 tcttaagctt gtttggtgca gtagcggtgc agaagaatat tcgtgatacc ggaataaacc
    15301 caccaaaaga aacacaggtt acccaggaag aatacagcga ataattcacg taagcccggt
    15361 cagtccaatg tgaacgggct tttacttaac tcactaatct gtttctgtcg attcgttgta
    15421 ccagcataga aagtaacaaa ctcgctgcca acgtcgcgca aaagatccaa ataatatcca
    15481 gtattggcca atttttaagc tcaattcccc gggtgcgcag cgcatggata atcaaggcgt
    15541 ggaatccgta tatacccaat gaatggcggg agattaagcc aagtccgcga atggtacgcg
    15601 tatccagcgt gtttttaacc agagtcaata gcgcgattgc gcagataaaa accatcggcc
    15661 cacagtaaag ataccaggta tcggcaaaat ttccgcgcca ctgcaattca tataatgtcc
    15721 cgcgagagat aataaaaacc cccgtcgcaa acagcgcggc gctcacccac gacagtgctt
    15781 tatgctgtgt gtccatcatc cctatagcgc ggcccaacat gccatacaga atgtagtaaa
    15841 aagtatcgcc attgatatat aagttaattg gcagccattc aaaaccgtca attttctgcg
    15901 gcactgtgtt tgggttagcg ataatgccaa tcaccaccat tagcaccagc aacatttttc
    15961 cgccgacgtt cttcacctga atcagcggtg aaaccagata aatcaccgca atcgcgaaga
    16021 aaaaccacaa gtggtaaaac actggctttt gcagcaggtt tttcagcgct aactccatat
    16081 tgatggaggt aaacagcgca atgtagagca gtgcgattgc gctataaaaa atcagacata
    16141 agccgatacg caagaaatgg cgcggctggg cgctgcgttc gccaaaaaag agatagccgg
    16201 aaatcatgaa aaatagcggc acgctgacac gagaggcaga attcagaaca ttggcgatat
    16261 cccaggtgac ggggctaaca ctatgagcat tggtcacata ccaggtagtg gtgtgaatca
    16321 tcaccaccat taaacacgct atccctcgca ggttatcaat ccagtaaatt ttgggctgca
    16381 tctgtgtctc tgtatctggt taaaaaaagt ctgaccgata aatcattcag ttggcgcact
    16441 ggaataatct gagttttatc actacagctt atagaggctt aaggaaattc gtaagatatc
    16501 agccactata ccgatataaa taataagact cacctgcaaa ccagacggta atttaatgat
    16561 gatgaacgct ttctttccgg caatggcgct tatggtgcta gtgggttgtt ctacaccgtc
    16621 acccgtgcag aaagcacaac gggtaaaggt tgatcctctg cgttcgttga atatggaagc
    16681 gttatgcaag gatcaggcgg caaaacgtta taacaccggc gagcaaaaaa tcgacgtcac
    16741 cgccttcgaa cagttccagg gaagctatga aatgcgcggt tataccttcc gtaaagagca
    16801 gtttgtctgt tcttttgacg cagatggcca ttttttgcat ctttccatgc gttaagccct
    16861 gctttttccc gtttcgtact gtatatcttc catccagcgg gtatactgat cccttccttt
    16921 aaatccacac gtatccagca cgaaataata tgcaaaagtt tgataccagg accttccagg
    16981 gcttgatcct gaccttacag gattactggg ctcgccaggg ctgcaccatt gttcaaccat
    17041 tggacatgga agtcggcgcg ggaacctctc acccaatgac ctgtctgcgc gcgctggggc
    17101 cagaaccgat ggcggctgct tatgttcagc cttctcgtcg cccgaccgat ggtcgctacg
    17161 gcgaaaaccc caaccgttta cagcactact atcagttcca ggtggtcatt aagccatcgc
    17221 cggacaatat tcaggagctg tacctcggtt ctctgaaaga gctgggcatg gacccgacta
    17281 ttcacgacat ccgtttcgtg gaagataact gggaaaaccc gactctgggt gcctggggac
    17341 tgggctggga agtgtggcta aacggcatgg aagtgacgca gttcacttac ttccagcagg
    17401 ttggtggtct ggagtgtaaa ccggttaccg gcgagatcac ctacggtctg gaacgtctgg
    17461 ccatgtacat tcagggcgta gacagcgttt acgacctggt ctggagcgac ggcccgctgg
    17521 gtaaaaccac ctacggcgac gtgttccatc agaacgaagt ggagcagtcc acttacaact
    17581 tcgaatacgc ggatgtggac ttcctgttca cctgctttga gcagtacgag aaagaagcgc
    17641 agcagctgct ggcgctggaa aatccgctgc cgctgccagc ctacgagcgt attctgaaag
    17701 ccgcccacag cttcaacctg ctggatgcgc gtaaagccat ctccgtcacc gagcgtcagc
    17761 gctacattct gcgcattcgc accctgacca aagcagtggc agaagcatac tacgcttccc
    17821 gtgaagccct cggcttcccg atgtgcaaca aagataagta agaggcggct atgtctgaga
    17881 aaacttttct ggtggaaatc ggcactgaag agctgccacc aaaagcactg cgcagcctgg
    17941 ctgagtcctt tgctgcgaat tttactgcgg agctggataa cgctggcctc gcacacggca
    18001 ccgttcaatg gtttgctgct ccgcgtcgtc tggcgctgaa agtagctaac ctggcggaag
    18061 cgcaaccgga tcgtgaaatc gaaaaacgcg gcccggcgat tgcccaggcg ttcgacgctg
    18121 aaggcaaacc gagcaaagcg gcagaaggtt gggcgcgtgg ttgcggtatt accgttgacc
    18181 aggctgagcg tctgactacc gataaaggcg aatggctgct gtatcgcgcc catgtgaagg
    18241 gcgaaagcac cgaagcactg ctgccgaata tggttgcgac ttctctggcg aaactgccga
    18301 tcccgaaact gatgcgttgg ggcgcaagcg acgtgcactt cgtgcgtccg gtgcacaccg
    18361 tgaccctgct gctgggcgac aaagtcattc cggcaaccat tctgggcatt cagtccgatc
    18421 gcgtgattcg cggccaccgc tttatgggcg aaccggaatt caccatcgac aacgccgatc
    18481 agtatccgga aattctgcgt gagcgcggga aagtcatcgc cgattacgaa gaacgtaaag
    18541 cgaagattaa agccgatgcc gaagaagcag cgcgtaagat tggcggtaac gctgacttaa
    18601 gcgaaagcct gctggaagaa gtggcttcgc tggtggagtg gccggtcgtt ctgaccgcaa
    18661 aattcgaaga gaaattcctc gcggtgccgg ctgaagccct ggtttacacc atgaaaggtg
    18721 accagaaata cttcccggtg tatgcgaacg atggcaaact gctgccgaac ttcatcttcg
    18781 ttgccaacat cgaatcgaaa gatccgcagc aaattatctc tggtaacgag aaagtcgttc
    18841 gtccacgtct ggcggatgcc gagttcttct tcaataccga ccgtaaaaaa cgtctggaag
    18901 ataacctgcc gcgcctgcaa accgtgctgt tccagcaaca gctgggtacg ctgcgcgaca
    18961 aaactgaccg catccaggcg ctggctggct ggattgctga acagattggc gctgacgtta
    19021 accacgcaac ccgtgcgggc ctgctgtcta agtgtgacct gatgaccaac atggtcttcg
    19081 agttcaccga cacccagggc gttatgggga tgcactatgc gcgtcacgat ggcgaagcgg
    19141 aagatgtcgc ggtggcgctg aatgagcagt atcagccgcg ctttgccggt gatgacctgc
    19201 cgtctaaccc ggtagcctgt gcgctggcga ttgctgacaa gatggatact ctggcgggta
    19261 tcttcggtat cggccagcat ccgaaaggcg acaaagaccc gtttgcgctg cgtcgtgccg
    19321 cacttggcgt gctgcgaatt atcgttgaga agaacctcaa ccttgatctg caaacgctga
    19381 ccgaagaagc agtgcgtctg tatggcgata agctgactaa cgccaacgtg gttgatgatg
    19441 ttatcgactt tatgctcggt cgcttccgcg cctggtatca ggacgaaggt tacaccgttg
    19501 acaccatcca ggcggtactg gcgcgtcgtc cgactcgtcc ggctgatttc gatgcccgca
    19561 tgaaagcggt atcgcacttc cgtacccttg aagcagctgc tgcactggcg gcggcgaaca
    19621 agcgtgtctc caacattttg gcgaaatctg acgaagtgct gagcgaccgc gtgaatgcct
    19681 ctaccctgaa agagccggaa gaaattaaac tggcgatgca ggttgtggtg ctacgtgaca
    19741 agctggagcc gtactttgcg gaaggtcgtt accaggatgc gctggtcgaa ctggcagagc
    19801 tgcgtgagcc ggttgatgct ttcttcgata aagtgatggt catggttgat gacaaagaat
    19861 tgcgtctcaa ccgtctgacc atgctggaga aactgcgcga actgttcctg cgcgttgcgg
    19921 atatttcgct gttgcagtaa taacgccgtt attaaaaagc ctgccatctg gcaggctttt
    19981 tttattatcg ctaaataata cagcaacctt taataatctt ctgctgaata aagattatct
    20041 catatattaa ttttatgagg tttttttagg attatatcaa ggagaagaaa caaacttatt
    20101 aagctagaat agccacgggt gcttgagact gtttgtctca ggtattcacc gaaaggcaga
    20161 cagagaaaag ccccacctga ctataaatca aagtgaggct accctatgcc tgaacaccat
    20221 aaggttagcc tcttactcgt tggaaatcaa cacagggggc tgggaatgcc gcagaaatat
    20281 agattacttt ctttaatagt gatttgtttc acgcttttat ttttcacctg gatgataaga
    20341 gattcactgt gtgaattgca tattaaacag gggagttatg agctggcggc atttttagcc
    20401 tgcaatttaa aagagtaaga gtcttcggcg ggaagttatt cccgcctttc ttacggcgtt
    20461 gcgcattctc attgcaccca aatttatttt tcacaaaaat aatactagat tttattacgc
    20521 gatcgattat ttatttcctg aaaaacaaat aaaaaaatcc ccgccaaatg gcagggatct
    20581 tagattctgt gcttttaagc agagattaca ggctggttac gttaccagct gccgggcctt
    20641 tagcgccgct ttcgatggtg aaggacactt tctgaccttc gtccagagat ttgtaaccat
    20701 cgttctggat agcagagaag tgtacgaaca cgtctttaga gccatcgtca ggagtgatga
    20761 agccgaagcc tttgtcagcg ttgaaccatt ttacgatacc agtcatttta ccggacatag
    20821 tgtattacct ttaataatta agtgtgcctt tcggcgatat ggcgtgcttt acagattttg
    20881 aagcgttaaa ggaatgtgca ctacgagggg tatcaacgat aactcttgaa gggacttgcc
    20941 ttactacact gctttaatgg tctgtacgtc aaaccgttga tgtgcattaa gccacgcatt
    21001 ggcgggtgat gcaacaatta tttttcatat ttatgattaa tcggttggaa aacggtcctg
    21061 tcatcaggac cgtaaacagc aataaagtgg ataaaagtct attccatcaa ctgcttactt
    21121 aatgccgggt tggcttgaat caaacgcatc aattttagtt cggcacttga aggcttcacg
    21181 cgtctggatt cccattcctt taccatggcg actgatacgc ccaaaacccg ggcgaaatca
    21241 tcgattttta accctgtccc ttttcgtaac tgctcaattt cggtacagga cgttgttctg
    21301 tgcgtcaggg taattttctg cgtttcatct ttaaaaacaa tctgttccag gctgctcaac
    21361 agctcatgca ttggatcttt atattccatt gaaaactcct caaatcacac tgcgggatcg
    21421 tgaattgcat cgaagctcat taagaatagt cgggaaaagc agacacagcg gggtgaagtc
    21481 agggcatgct ctggctatga ttcgattcag cctgtcattt agcgtgactt atagccacct
    21541 aacgatctga taaatgccga tatatctctt accactcctg ttttgtaaag atttcgaaaa
    21601 tctaaccttc tgcgaaggcg taaatctctg gctacaaaaa aggcaacggc actatcattt
    21661 tttaacaatg aaacaataaa ttaaccttgt ttcattttct ccatgatttt accaggaaga
    21721 ttacgtagaa agggtatcct gcaagcctgt cctggactat ccttgtcacg tcagacacgc
    21781 gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc
    21841 tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg tagttagcct gagtgggtgt
    21901 tttgataaag aaggcgatca gcgtaaagcg tttatcgact tcctgcagaa tacagtgatg
    21961 cgtagcggtg aacgtctacc aaccctgact gccgatcaga aaaaacagtt tggtcctttt
    22021 gtctctgatt acgcgattct gtatggttat tctcagcagg tgaatcaggc gatggattcc
    22081 ggtctgcgcc cggtcgtaga cagcgttaac gcaattcgcg taccgcagga ttacgttacg
    22141 caaagcggtc cgctgcgtga aatgaacggt tcgctgggtg tgctggcgca acaactgcaa
    22201 aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa aacagaccga tgatttgaaa
    22261 ccggtctttg atcaggcatt caccaaagtg gtaacgacgc cagctgatgc attgcaaccg
    22321 ttgattccgg ctgcacaaac tttcacgcaa caactggtga tggttggaga ctacattgct
    22381 cagcaaggta ctcaggtgag ctttgtcgca aatggcatcc agttcccgac ttcacagcag
    22441 gcaagtgaat ataacaaact gattgcgcca ttaccggcac aacatcaggc gtttaatcag
    22501 gcctggacta cagcagttac tgcaacccaa taaagagtaa aagcccgagc gattatcggg
    22561 ctttacttca cgcagtcgcg gcttagtccg cgacgtgcgg attcacacag ttcttctcaa
    22621 cctttccttg taacgcatca atcaaattat ccacggcaca ggcggccatg ccataacgcg
    22681 tctcatgggt ggcagatcca atatgcggta ctgcgacgac gttggccatt gagagcaacg
    22741 gcgaatctac ggacagtggc tcttgttcga agacatccag cccggcggcg tgaatttcgc
    22801 ctttctgcaa tgcagcaatc agtgcatttt catcaaccac cgggccacgt ccggcattaa
    22861 tgaaaatggc ggaggatttc attttggcga attgttctgc gccaaacaga tgatgcgtct
    22921 catcagttaa cggcaggatc aggcaaacga aatctgactc ttgtaacaga gtatccaaat
    22981 cgcagtagcg ggcgttgaag cgttcttctg cttctttatg gtggcggcgc gcgttataga
    23041 ggatgggcat gttgaagcca aagtgcgcac gttgtgccag cgccataccg atccgtccca
    23101 tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc
    23161 tcgcggtcca ttcgcctgct tttacccgtt ctgctacctc cacaacccga cgagcggtag
    23221 acaacaccag cgccatcagc gtatcggcga cggtttctgt taatacggtt ggcgtgtgca
    23281 tcagcagaat ttttcgggcg gtaagcgcat cgacatcaaa attgtcatag ccgacggaga
    23341 tcgttgatgt ggcacgcagt ttcggcattt tttccagcaa tgcggcatca acattctcgt
    23401 ttgaacccag taaaccttca gcttcggcaa aaattgctgc attttgttcg acggtttgtg
    23461 ggctgaggtt tgccacctgg tgaacggtga aatgctcttg caggcgttgc agtaaatcat
    23521 caggtaaggc tttgtagagg ataacggacg gcttcatgct tctctccatt cactgataag
    23581 gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa gaatgccagg tgatttctgc
    23641 atcacctggc atgaaaggga ttacagcggg cttaaggtaa tttctacacg gcggttttgc
    23701 gccttacctt ctgcggtgct gttgctggcg attgggttag ccgggccaag gccctgagta
    23761 cggatgcggc tggcgtccac gccctgggtg atcaacgcgc tggcaacgga atccgcacgt
    23821 tgctgggaga gacgcatgtt caggtcgtga ccacccgtgc tgtcggtata accaatcacg
    23881 ttaaccgccg ttttcggata ctctttcagc accattgcca cgccggtcag ggtgttagcg
    23941 cccgcaggtt tcagggtcgc gctgctgctg tcgaaggtca cattgttcgg catattgagg
    24001 ataatgttat ccccgctgcg ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc
    24061 ttcgcttcct gcacatccat gtaataaccc acgccgccgc ccagagctgc gcctgctgct
    24121 gcgccaatca gcgcgccttt accgcgatct ttcttcgaag aagagagcgc accaataccc
    24181 gcgcccacga gagagcccag acctgcgccg atagcagatt tacctgcttc gcgttcgccg
    24241 gtgtaagggt tagttgtgca gccagatacc gccagagcgc cactcactac ggcggcaata
    24301 agataaacac gtttcttcat tgttaatcct taataacctt tttattcttt gccacgggtt
    24361 ccgtggcggg agattatgcc gcgtgaacat gaagattatt cctgggaata ctcggaaatt
    24421 tgtaagtaat atttaactgc tcaatacatc taacctttca ggagttttcg gtttggccaa
    24481 ctcatcctca cgatattccg ttctgactgc cgcccattgg gggcccatgc tggttgaaac
    24541 cgacggcgaa accgtgttta gctcgcatgg cgcgttagcc acaggaatgg aaaactcctt
    24601 gcagagcgcg gttcgcgacc aggttcacag caatacacgg gtacgatttc caatggtgcg
    24661 caaaggcttt cttgcgtcac cggaaaatcc gcaaggcatt cgtgggcagg atgaatttgt
    24721 tcgcgtgagt tgggatgagg cgctggatct tattcaccaa caacataaac gcattcgtga
    24781 ggcttatggt ccggcatcga tttttgctgg ttcctacggc tggcgttcaa acggcgtgct
    24841 gcataaggcc tcgacattat tacaacgcta tatggcgctg gcaggcggtt ataccgggca
    24901 tctgggggat tattcgaccg gcgcggcaca ggcgatcatg ccgtatgtcg tgggtggtag
    24961 cgaagtttat caacagcaaa ccagttggcc gctggtgctg gaacatagcg atgtcgtggt
    25021 gttgtggagt gctaacccac tcaatacgct gaaaattgcg tggaatgcat ctgatgagca
    25081 ggggttttct tacttttccg cactgcgtga cagcgggaaa aagctgatct gtattgatcc
    25141 aatgcgatcg gaaaccgtcg atttctttgg cgataaaatg gagtgggtgg caccgcacat
    25201 gggcaccgat gttgcgctga tgctggggat cgcccatacg ctggtggaaa atggttggca
    25261 cgacgaagcg tttctgacgc gttgcaccac aggttatgcc gtcttcgcct cttatttgct
    25321 gggcgagagt gacggaatag cgaaaaacgc cgaatgggcg gcagagattt gtggggttaa
    25381 cgcagcgaaa atccgcgagc tggcggctct tttccaccaa aacacgacca tgctgatggc
    25441 tggctgggga atgcagcgcc aacagtttgg tgagcaaaaa cactggatga tcgtcacgct
    25501 ggcggcaatg ttggggcaaa tcggcacacc cggcggcggt tttggtcttt cttatcattt
    25561 tgccaatggt ggtaacccca cgcggcgttc tgcggtgctc tcttccatgc agggcagctt
    25621 gccgggtggc tgcgatgcgg tggataaaat ccctgttgcc cgcattgttg aagcactgga
    25681 aaaccctggt ggcgcatatc aacacaacgg tatgaaccga catttcccgg atattcgttt
    25741 tatctggtgg gcgggcggtg ccaactttac tcatcatcag gataccaatc gtctgatccg
    25801 tgcctggcaa aaaccggagc tggtggtgat ctctgaatgc ttctggacgg cggcggcgaa
    25861 acacgcggat atcgttctgc ctgcgactac ctcttttgag cgtaatgatc tcaccatgac
    25921 cggtgattac agtaatcagc atctggtgcc gatgaagcaa gtggtgccgc cacgctatga
    25981 agcgcgtaat gattttgatg tttttgccga gttgagtgaa cgttgggaga agggcggcta
    26041 tgcgcggttt acggaaggaa aaagtcagct gcaatggctg gaaacgtttt ataacgttgc
    26101 ccggcagcgc ggggcaagcc agcaggttga attgccgcca tttgctgagt tctggcaagc
    26161 caaccagtta attgagatgc cggaaaaccc ggacagcgag cggtttattc gcttcgctga
    26221 tttttgccgc gatccgctgg cgcatccgtt aaaaaccgcc agcggcaaga ttgaaatctt
    26281 ctcacagcgt attgccgatt acggttaccc ggattgccct gggcatccaa tgtggctgga
    26341 gccggacgaa tggcagggca atgccgaacc agaacagttg caggtacttt ctgcccatcc
    26401 ggcgcaccgc ctgcacagcc agctgaatta cagttctctg cgcgaattgt acgcggtagc
    26461 aaaccgtgag cctgtcacca ttcatcctga cgatgctcag gcgcgcggca taaccgaagg
    26521 ggatatggtg cgggtgtgga actcccgtgg gcagatcctt gccggagcgg tcattagcga
    26581 gggaattaaa cctggcgtga tttgcattca cgaaggggca tggccggatc tggatttaac
    26641 cgctgacggt atttgtaaaa acggcgcagt gaacgtgctg accaaagatc tccccaggtc
    26701 gcggctgggg aatggctgtg cgggtaatac ggcgctggca tggctggaaa aatacaacgg
    26761 tccggaactg acacttacag cgtttgaacc accggccagc tcataatcca ggtgggtagt
    26821 tgggtttcat cctgccatgc gcaatcgaca atgtgaaaac cctgcgcacg gtaaaaatct
    26881 atcgccggtt gatttttttg ataaacctcc agcatcagat ggggatagcg ctgctgcaca
    26941 tactgcatca gcgccttacc aataccgcgt ctgacggcct tcggtgcgac aaacattgct
    27001 gccagaaatc ggccttccat aatgctgaca aaaccgagaa gcttaccgtc ctcttcccag
    27061 acccagtttt gcgcgttggc aagataggca tcccgcacca gcggaatgca gtcacgccag
    27121 taattcgctt ttataaaggg atgcccccag gttgtacttt ccagccacag ttcgaggatc
    27181 gcggggagtt ctgaacgttg cgcttcccga atcatggttt atttcccgga tagcaacagc
    27241 agccaaccac atgatcattc accagcccac atgcctgcat aaaggagtaa cagattgtgg
    27301 tgccgacaaa cttaaaacca cgttttttca gtgccttaga tagggcgtcg gaggcggacg
    27361 tagatgtggg aatttcgctc aacgttgtgg cttgtgtcac ctgtggctga tgatttacaa
    27421 acgaccagac aaagtcggca aacggttcgc cgttctgttc catttgcagg tacgcccgcg
    27481 cattaccaat aattgcctga attttccctc gatggcggat aatcccggcg tcctgtacca
    27541 gtctttcgac atcctcttcc tgcattgctg cgaccttcac cggatcgaac tgatgaaagc
    27601 aggcgcgata gttttcgcgt tttttgagga cggtgatcca cgataaccca gcctgctgcc
    27661 cttcaaggca gatcatttcg aacagttttt tactgtcagt ttcaggcacg ccccattcat
    27721 tatcatggta ggcaatataa agcgggtcct gactcaccca gccgcaacgt tccatacttt
    27781 ccctcgctat gtacccgatc gtaaaaattt cagccgcttt tcttgacgca tcggtgaaaa
    27841 agtcaatatt taatacaggg ctactatgcc cgatatcctt catgcaatga caataatatt
    27901 gccgaattcg gctcttccct ggagtcgtgt taatgatcat aaaaaaaagc ggtggtcgct
    27961 ggcagctaag cctgctggcg agcgtggtaa tcagtgcctt ttttctcaac acagcttacg
    28021 cctggcaaca agaatatatc gttgatacgc aacccggaca ttccacagag cgttacacct
    28081 gggatagtga tcatcaacct gattacaacg atattttgtc gcaacgtatt caaagtagcc
    28141 aaagggcgct gggactggaa gtcaatctgg cggaagaaac tcctgtggat gtgaccagca
    28201 gtatgagtat gggctggaat tttcctttat atgaacaggt tacaaccggc ccggtcgcgg
    28261 cattacatta cgatggcaca accacctcga tgtataacga gtttggcgac agtactacca
    28321 cgctgaccga tccgttatgg catgccagcg tgagtagctt aggctggcgt gttgactccc
    28381 ggcttggcga tctccgaccc tgggcgcaaa tcagctataa ccagcaattt ggcgagaata
    28441 tctggaaggc gcaatcaggc ctgagccgga tgacggcgac aaaccagaac ggcaactggc
    28501 tggatgtcac cgtaggcgct gatatgttgc tcaatcaaaa tattgccgcc tatgccgcgc
    28561 taactcaggc agaaaatacc actaataata gcgactatct gtatacgatg ggggttagcg
    28621 ccagatttta acgtaacagt cacaattgaa accattgaat aacaacagtt gtggcgatag
    28681 tgggtgctaa cttaccaaat aataaatttg gtgaataatt gtcgcgtcat tcattcctga
    28741 actaaggcat ttcattccgt tctgatggca tttcatgccg tttttcccca ggcataaaat
    28801 gcacttcgtt atggttgtcg gcagagattt ttccttttta ttactgcagg aatactgcca
    28861 tgacaccttc aaattatcag cgtacccgct ggctgacact catcggtact atcattaccc
    28921 agtttgcact ggggtcggtt tatacctgga gcctgtttaa tggcgcgctt tctgccaagc
    28981 tggatgcgcc ggtaagccag gtcgctttct ctttcggctt gttaagtctg gggctggcaa
    29041 tttcgtcttc tgttgcgggc aaattgcagg aacgttttgg cgttaaacgc gtcaccatgg
    29101 cttccggcat tttgctggga ttaggcttct tcctgaccgc gcattccaac aacctgatga
    29161 tgctgtggtt aagcgccggt gtgctggtcg gtctagcgga tggcgcgggt tacctgctga
    29221 cgctctctaa ctgcgtgaag tggttcccgg agcgtaaagg tctgatatcc gcgttcgcta
    29281 tcggttctta tggtctgggt agcctgggtt tcaaatttat cgacacgcag ctgctggaaa
    29341 cggtgggtct ggaaaaaacc tttgtgattt ggggagcgat tgcgctggtg atgattgttt
    29401 tcggcgcaac gttaatgaaa gacgcaccaa aacaggaagt gaaaaccagc aatggcgtag
    29461 tggagaaaga ttacacgctg gcagagtcga tgcgtaaacc gcagtactgg atgttagcgg
    29521 taatgttcct gactgcctgc atgagcggcc tgtacgtgat tggggtagcg aaagatatcg
    29581 cccaaagtct ggcacacctt gatgtggttt ccgcagccaa tgcagtaacg gttatttcca
    29641 tcgctaacct ttcaggtcgt ctggtgctgg gcattctgtc tgacaaaatt gcccgtatcc
    29701 gtgttatcac cattggtcag gtgatttcgc tggtgggtat ggcggccctg ctgtttgcac
    29761 cattaaatgc agtgacgttc tttgcagcga ttgcctgcgt ggcatttaac tttggcggca
    29821 ctattaccgt ctttccatca ctggtcagtg agttctttgg cctcaataac ctggcgaaaa
    29881 actacggtgt gatttacctc ggtttcggta tcggtagcat ttgtggttcg attatcgcct
    29941 cactgtttgg cggcttctat gtgaccttct acgtgatttt cgccctgctg attctgtcat
    30001 tggcgctttc tacgacaatt cgtcagccag agcagaaagt attgcgtgag gcgcatattt
    30061 agaaatatgt tgctactgaa ataaatgcga ggagggagac ttcaaagtct cccttttttt
    30121 atggacgcat attaaaagaa caataatatt tacaccacaa atatatttta tttgtttata
    30181 tatcatgtaa atataaatat attcctaaaa aaatagtcgt tggaagcgtt tttgtgtgca
    30241 attaattgaa gttgaagtac atttcaattt attgcaaaaa ctaatcttca gtaaaaataa
    30301 ttttcttttt tagcaccatt tgtataatct gcgccccgtt gtatgaacaa catcaagcat
    30361 atttatctat ataaatggag ttataaatga aaaaggttct gtttgcctta tctgcgcttg
    30421 cgctgacctc tacttccgta ttcgcagctg atgcgggcga cggtactgtt aaattcaccg
    30481 gtgagattgt tgacgccgct tgtgttgtgt ctccagacac tcaaaaacaa gaagttgttc
    30541 tgggtcaggt gaacaagtct gtatttactg ctatgggtga taaatccaca gcaacgccgt
    30601 tcaaaatcaa actggaaaac tgcgatatct ccactttcaa aaatgtggaa atcagcttta
    30661 acggcgttgg cgatgcagac aacagcaaac tggtttctgt aagcactgaa ccaggtgccg
    30721 caactggcgt aggtatcggt atttatgata acaccaatac cctggttgac ctgaatactg
    30781 gtaaatctgc tactgtcctg aaagaaggtc agaccgttct gtatttcacc gctaactatg
    30841 tctctaccaa agatgcagta accacgggtt acggtaacgc tgaagtcgac ttcaacctga
    30901 cttacaacta atcgatcctg acggaacgat catctctcaa tggcaacaga gactgtgttg
    30961 ccattattca caccaatgac tcagggaccg aatcatgaaa cgtattagct ttgctgtgct
    31021 ggcactttta atgaccgcac aacagtgttt tgctggtggc gtagccttaa gcacgacgcg
    31081 tgttatttat gatggcggca agaaagaagc gtcgctgacc gttgaaaacc ataataaaaa
    31141 tgaagagttt ttgatccagt catggattga tgatgtgaat agcaataaaa aaacaccgtt
    31201 tattattaca ccgccattat tcaaacttga tcctgataaa aataacgtct tacgtatcgt
    31261 caatattgaa gataccttac ctaaagatcg tgaatccgtt tactggatta atgtaaaggc
    31321 cattcctgcg aaaagtgctg aaaatgaaaa taaaaacgtc ttacagatag ccgttcgcac
    31381 ccgtttgaag ttgttctatc gtccaaaagg tttgccggga agcaacctcg aggccatgaa
    31441 acagttacgt ttccagcgtc aaaataacca aattgtggtt aataacgact ccgcttataa
    31501 cctgaccttt aatcagtttt ccattaatgg acaaaagatt gaacgggcgg gaatggttct
    31561 cgcgaaagga aagttaaaca ttaaccttcc agccggtaca ggcaacgcgc acgaagtgaa
    31621 atacagcatt attaatgatt tcggtgttgc tggcgaaatg ctgacgaagc cgattaactg
    31681 atccttttac aaggcgcttt tactatgcat tggatgcatt tacctctcta tcactacaga
    31741 gcgcattttt ccttctctct gctggcatta acgattgcca gctcgttacc tgcgtatggc
    31801 ggaaaattta accctaaatt tctcgaaaac gttcagggta tcgatcaaca catcgacttg
    31861 tcggtatacg actcacctgt cggccagcaa atccccggta aataccgggt ttcggtgttt
    31921 gtaaacgaag aaaaaatggc ttcccggacg ctggatttta gtaccgccag cgaagcgaaa
    31981 cgcaaagcca gcggtgagtc gcttatgcct tgccttagcc gggtgcaact ggaagaaatg
    32041 ggcgtgcgcg ttgacagctt tccggcgctg aagatgtcac caccagaagc ctgcgtagcg
    32101 tttgatgaaa tcattcccca ggccaccagc cgctttgatt ttaatactca gacgctgcat
    32161 ctttcctttc cacaggcggc aatgatgatg accgctcgcg gcacggttga tccttctcgc
    32221 tgggacgaag gaatacctgc gctgttgctg gattacagct ttagtggcag caatggacgc
    32281 aatgaaggta caggttcatc ttcagattcg accagtgaca gttattacct gaacctgcgc
    32341 agcggtttga acgttggtcc gtggcgttta cgtaataaca gtatctggaa ccgtaccgat
    32401 ggcaaaaacc aatgggataa cgttggtaca tcgttaaacc gggcaattat tccacttaaa
    32461 tcacagataa ctctgggtga taccgccacg cctggcgaga tttttgatag cgtacaaatg
    32521 cgtggggcgt tactggcatc cgacgatgaa atgttgccgg acagccagcg tggctttgct
    32581 ccggtagtgc gcggcatcgc caaaagcaac gctgaagtga gtatcgaaca gaacggctat
    32641 gtcatttatc gcacttttgt tcagccgggt gcatttgaga ttaacgatct ctacgcaacc
    32701 tccggcagcg gcgacctgac ggtgatcatt aaagaagccg acggaagtga acaacgtttt
    32761 atccagccgt tttcggcggt ggcgattttc cagcgtgaag ggtatctgaa atacagtctg
    32821 gctgccggtg aatatcgtgc tggtaattac gacagcgata aaccacattt cgggcagttt
    32881 accgccatgt acggcttgcc gtggggtatg accgcctacg gtggtgcatt gttgtcggca
    32941 gattacaacg ctttagctct gggcctgggg aaaaacttcg gtaccattgg cgcagtatcg
    33001 gtggatgtca ctcaggcgaa aagtcagtta cgcaacaatg aaaaagagga aggccagtct
    33061 tatcgcttcc tctattcgaa aagttttgag ggtggcactg acctgcgtct gttgggctac
    33121 aaatactcaa ccagcggcta ctacaccttc caggaagcaa ctgatgttcg cagtgacgcc
    33181 gacagtgatt accgccgtta tcacaagcgc agccagatac agggcaatat tacccagcaa
    33241 ctgggagact acggctcggt ttatttcaat atgacgcagc aggattactg gaacgttgac
    33301 ggtaaggaaa actccttaag cgccggttat cacggtcaca ttggtcgggt gaattacagc
    33361 gtggcctata cctggacgag aagtccggaa tgggaagaag acaatcgctt gtggtcattc
    33421 agtgtttcca tccctctggg gggcgcgtgg agcagttatc gcatgactac cgaccagaat
    33481 ggcaagacgt cgcaacaggc ctctgttagc ggcacgttac tggaagatcg taacctgagc
    33541 tataacgttc agcagggtta caccagtaat ggcgtaggct acagtggcag cgtcaacatg
    33601 gggtatatgg gtggctctgg taacattgac gtaggctata actattctaa agataaccaa
    33661 caggttaact atggcgtgcg cggcggtgtc atcgttcaca gtgaaggtat aacgctttcg
    33721 cagcctttag gcgaaagtct ggcgattgtt tctgcaccgg gcgcgcgtgg tggacatgtg
    33781 gttaacagca gcggcgtaga agttgactgg atgggcaacg ccgtggtgcc ttatctcaca
    33841 ccatatcgtg aaaccattgt ggaattgcgt agcgacacgt tggggcaaaa cgttgaacta
    33901 caggaagcgt tccagaaagt cgtgcctact cgtggtgcag ttgtgcgttc ccgctttgat
    33961 acccgcgtcg gttaccgtgt gctgatgagc ttaaaacagg cgaatggcaa cgcagtaccg
    34021 tttggtgcaa ccgcagcgtt aattgatgag agcaaacctg ccagcagcat tgtgggtgaa
    34081 gaaggacagc tttatatcag cgggatgcca gaagagggcg aattgcaggt gagctggggg
    34141 aacgagcagg cgcaacgttg ccgtgtgcca ttccgcttgc cagagaacaa ggacaatgcg
    34201 gctatcgtga tggttaatgc ggtatgcgag aaataaggat atttagatga aaattaaaaa
    34261 tttgttggca ggtatggtgc tgttagggac gagcactttt gctggaaata tatgggctgc
    34321 ggattgggga ccatgtcaga catcaaatgg ggtggcccat gagtatagtt ttgatttcgt
    34381 acagactatc caggtacctt cagaaaataa ggctgggaaa atattaactc agccctttgc
    34441 tttaggtaca aaatattccg cttattgtga atgcccggat cccattccag ataatggagt
    34501 tgtgacttat tttaaaggtg taacgcttct gcctgagcct ggaactgtag atgggtacta
    34561 taaattcaat aattacattg atattctcac caaaatatat gtctacacac aattagatat
    34621 cccggtgcct tttaccgatc gaagcaatgg cacggcacag aaagaatgta cgccctatac
    34681 tgctaacaat tgggggacag gtggtaaagg aagtatcagt atttatattt cacatccttt
    34741 tgttgggcaa atgattattc ctaaaacacg agttgcgtcc ctctttggga caaaaaagaa
    34801 aggtgtttac aatgatagtc agcccatggc caatgtcagt atttccggga gtattacagt
    34861 tactcaaggc tgtgaacttg ccgctggcac cgtactggat attccattcg gagaatatca
    34921 ggcccatgat tttaaagggc gtgccggaca accaccacaa aatgttcaga aggtacaaaa
    34981 agaattaagc tttgactgca ataatatttc agatggcgtc aaaatttatc tcagtattga
    35041 tgcaacacca aatactgcct atcccagcgc aattgattta ggcaacgccg atgttggtgc
    35101 agtcattgag gacggtaaag gtaatattct taagccgaat gacagtaata gcttattaga
    35161 aatgaaccca ggctcgctgt atgaagatgt caaacgcaaa gcgacaacca caattaccgc
    35221 ttatcccgtt agcactaccg gaaaattacc cgctgccggg gattatagcg gtattgctac
    35281 catacatgtc gaactggagt aacagtcatg aagcgaacaa tactttcgtt atgcctgctg
    35341 cctctttcct tttcgtcatt cgcggcaacc acggatttag ggccgaaagg aacattgaaa
    35401 ttcaccctta aaatcagcca gggtgcttgc gaactggtga aagacagcgt tgaggttgac
    35461 atgggcaccg cggttctcaa aaagccggta agaatcggaa cggaaattaa tcccactcct
    35521 ttttccatcg gcctgaaaaa ctgtagcgaa gtggtcagag cctacgtgac tatggacggt
    35581 acacccaatg cagacgactc aaatcttttt gcgctggatg cgggtggcgc tacaggcgtt
    35641 gggctgaaaa tcaaaaccgc tgctggagta caacagttcc cggaaaatac aacgccgaca
    35701 ccggttgagt ttgcaattaa cggtgatggc gcacatcaac tgaactatat cgccagctac
    35761 gtgcctattc gcgccgatgc aactattggt cgcgcagatg caacggtcga ctttactgta
    35821 cagtacgaat aatttcacat cttaagggcc agttaactgg ccctttttac tgctttttgt
    35881 aacgctaaag tctcttttca aacttgcatt tttgtaaatt tgtgcttcat gcacactctt
    35941 tccccacact ttttcgcttt gctgtggtct acttattcgc gcgtgtagat tttacttatc
    36001 tgactacctc cgcacttttc ccctgccggg cctgaaaagc cgctaagcag ggtgttatca
    36061 cctgtttgtc cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg
    36121 agctttttgc ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttatcgccgc
    36181 ttcggcagct atgcgcacga ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg
    36241 gccgccaccg tactggtgac cttcttttta ctacgtcttc tatcgctgtt tggccgccgc
    36301 agctggcgta ttctggcgtc gctggtggtg ctcttttccg caggtgccag ctattacatg
    36361 accttcctta atgtggtcat tggttatggc atcatcgctt ccgtcatgac caccgatatc
    36421 gacctgtcaa aagaagttgt tggtctgaac tttattctct ggttaatcgc cgttagtgca
    36481 ttgcctctta tctttatctg gaataaccgc tgtcgctaca ccttgctccg acaactgcga
    36541 accccagggc agcgtattcg cagcctggcg gtcgtcgtac tggcgggtat tatggtttgg
    36601 gcaccgattc gtttgctgga tatccagcag aagaaagtgg agagggcgac cggcgttgat
    36661 ttgccgagtt atggcggtgt cgtagcgaac tcttatctgc catcaaactg gctttctgcg
    36721 ttggggctgt atgcctgggc gcgggtcgat gaatcttccg ataataattc attgcttaat
    36781 ccggcgaaga aattcactta tcaggcaccg caaaacgttg atgacactta tgtcgtgttt
    36841 atcatcggtg aaaccacgcg ttgggaccat atgggcattt tcggctatga gcgtaatacc
    36901 acgccgaaac tggcccagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc
    36961 gcaaccaaac tctcactgcg ttgcatgttt gtacgtcagg ggggcgcgga agataatccg
    37021 cagcgcacat taaaagaaca gaacattttc gcggttctga agcagttagg attcagttct
    37081 gacctctacg ctatgcagag cgaaatgtgg ttctacagca acacgatggc ggacaacatt
    37141 gcttatcgtg agcagattgg tgcggagcca cgtaatcgtg gcaagccggt agatgatatg
    37201 ttgctggtag acgaaatgca gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc
    37261 attctgcata ccaaaggttc gcattttaac tacacccagc gttatccgcg tagcttcgcg
    37321 cagtggaagc cggaatgtat tggtgttgat agcggctgta ccaaagcgca gatgatcaac
    37381 tcctatgaca actcggtgac ctatgtggat cactttatct ccagcgtgat tgatcaggtt
    37441 cgcgataaga aagcgattgt gttctacgca gctgaccacg gtgagtcaat taatgaacgc
    37501 gagcacctgc acggcacgcc gcgtgaactg gcaccgccgg agcagttccg cgtaccgatg
    37561 atggtctgga tgtcagataa atatctggaa aatccggcca atgctcaggc atttgcgcag
    37621 ctgaaaaaag aagccgacat gaaagtgcca cgccgtcatg tagagctgta cgacaccatc
    37681 atgggttgtc ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgt
    37741 cacatcccgc agacaaaagt ggcagcggct aactaagcgg cctgctgact ttctcgccga
    37801 tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc
    37861 ccgcattcgg tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag
    37921 gttc
//
LOCUS       DAHBHC010000046        37888 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHBHC010000046 DAHBHC010000000
VERSION     DAHBHC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.046"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            195..1358
                     /locus_tag="M2A35_002775"
     CDS             195..1358
                     /locus_tag="M2A35_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7199058.1"
                     /translation="MSLFRRNEIWYASYSLPDGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEIGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(1374..1450)
                     /locus_tag="M2A35_002776"
     tRNA            complement(1374..1450)
                     /locus_tag="M2A35_002776"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1414..1416),aa:Arg,seq:tct)
     gene            1693..2325
                     /gene="fimZ"
                     /locus_tag="M2A35_002777"
     CDS             1693..2325
                     /gene="fimZ"
                     /locus_tag="M2A35_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCB7199059.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(2328..2843)
                     /gene="sfmF"
                     /locus_tag="M2A35_002778"
     CDS             complement(2328..2843)
                     /gene="sfmF"
                     /locus_tag="M2A35_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCB7199060.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(2854..3861)
                     /gene="fimH"
                     /locus_tag="M2A35_002779"
     CDS             complement(2854..3861)
                     /gene="fimH"
                     /locus_tag="M2A35_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCB7199061.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(3874..6482)
                     /locus_tag="M2A35_002780"
                     /pseudo
     CDS             complement(3874..6482)
                     /locus_tag="M2A35_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(6513..7205)
                     /gene="fimC"
                     /locus_tag="M2A35_002781"
     CDS             complement(6513..7205)
                     /gene="fimC"
                     /locus_tag="M2A35_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCB7199062.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(7425..7967)
                     /gene="fimA"
                     /locus_tag="M2A35_002782"
     CDS             complement(7425..7967)
                     /gene="fimA"
                     /locus_tag="M2A35_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCB7199063.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            8448..9314
                     /gene="folD"
                     /locus_tag="M2A35_002783"
     CDS             8448..9314
                     /gene="folD"
                     /locus_tag="M2A35_002783"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCB7199064.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            9316..9528
                     /gene="ybcJ"
                     /locus_tag="M2A35_002784"
     CDS             9316..9528
                     /gene="ybcJ"
                     /locus_tag="M2A35_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCB7199065.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            9636..10157
                     /locus_tag="M2A35_002785"
     CDS             9636..10157
                     /locus_tag="M2A35_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCB7199066.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(10193..11578)
                     /gene="cysS"
                     /locus_tag="M2A35_002786"
     CDS             complement(10193..11578)
                     /gene="cysS"
                     /locus_tag="M2A35_002786"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCB7199067.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            11752..12246
                     /gene="ppiB"
                     /locus_tag="M2A35_002787"
     CDS             11752..12246
                     /gene="ppiB"
                     /locus_tag="M2A35_002787"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCB7199068.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            12249..12971
                     /gene="lpxH"
                     /locus_tag="M2A35_002788"
     CDS             12249..12971
                     /gene="lpxH"
                     /locus_tag="M2A35_002788"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCB7199069.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKAEALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            13089..13598
                     /gene="purE"
                     /locus_tag="M2A35_002789"
     CDS             13089..13598
                     /gene="purE"
                     /locus_tag="M2A35_002789"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCB7199070.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            13595..14662
                     /gene="purK"
                     /locus_tag="M2A35_002790"
     CDS             13595..14662
                     /gene="purK"
                     /locus_tag="M2A35_002790"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCB7199071.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14799..15692)
                     /locus_tag="M2A35_002791"
     CDS             complement(14799..15692)
                     /locus_tag="M2A35_002791"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCB7199072.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKAYGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(15689..16504)
                     /locus_tag="M2A35_002792"
     CDS             complement(15689..16504)
                     /locus_tag="M2A35_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCB7199073.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            complement(16515..17774)
                     /locus_tag="M2A35_002793"
     CDS             complement(16515..17774)
                     /locus_tag="M2A35_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCB7199074.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(17784..19451)
                     /locus_tag="M2A35_002794"
     CDS             complement(17784..19451)
                     /locus_tag="M2A35_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCB7199075.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            19768..20817
                     /gene="allD"
                     /locus_tag="M2A35_002795"
     CDS             19768..20817
                     /gene="allD"
                     /locus_tag="M2A35_002795"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCB7199076.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            20839..22074
                     /gene="allC"
                     /locus_tag="M2A35_002796"
     CDS             20839..22074
                     /gene="allC"
                     /locus_tag="M2A35_002796"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCB7199077.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDICIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYWVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            22085..22870
                     /gene="allE"
                     /locus_tag="M2A35_002797"
     CDS             22085..22870
                     /gene="allE"
                     /locus_tag="M2A35_002797"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCB7199078.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(22999..24144)
                     /gene="glxK"
                     /locus_tag="M2A35_002798"
     CDS             complement(22999..24144)
                     /gene="glxK"
                     /locus_tag="M2A35_002798"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCB7199079.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(24166..25467)
                     /locus_tag="M2A35_002799"
     CDS             complement(24166..25467)
                     /locus_tag="M2A35_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCB7199080.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(25524..26885)
                     /gene="allB"
                     /locus_tag="M2A35_002800"
     CDS             complement(25524..26885)
                     /gene="allB"
                     /locus_tag="M2A35_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HCB7199081.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(26945..28399)
                     /locus_tag="M2A35_002801"
     CDS             complement(26945..28399)
                     /locus_tag="M2A35_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCB7199082.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(28569..29447)
                     /gene="glxR"
                     /locus_tag="M2A35_002802"
     CDS             complement(28569..29447)
                     /gene="glxR"
                     /locus_tag="M2A35_002802"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCB7199083.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(29547..30323)
                     /gene="hyi"
                     /locus_tag="M2A35_002803"
     CDS             complement(29547..30323)
                     /gene="hyi"
                     /locus_tag="M2A35_002803"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCB7199084.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(30336..32117)
                     /gene="gcl"
                     /locus_tag="M2A35_002804"
     CDS             complement(30336..32117)
                     /gene="gcl"
                     /locus_tag="M2A35_002804"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCB7199085.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKLAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(32207..33022)
                     /gene="allR"
                     /locus_tag="M2A35_002805"
     CDS             complement(32207..33022)
                     /gene="allR"
                     /locus_tag="M2A35_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCB7199086.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(33100..33582)
                     /gene="allA"
                     /locus_tag="M2A35_002806"
     CDS             complement(33100..33582)
                     /gene="allA"
                     /locus_tag="M2A35_002806"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCB7199087.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            33812..34738
                     /gene="allS"
                     /locus_tag="M2A35_002807"
     CDS             33812..34738
                     /gene="allS"
                     /locus_tag="M2A35_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCB7199088.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            34807..35901
                     /gene="mnmH"
                     /locus_tag="M2A35_002808"
     CDS             34807..35901
                     /gene="mnmH"
                     /locus_tag="M2A35_002808"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCB7199089.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            36017..36382
                     /locus_tag="M2A35_002809"
                     /pseudo
     CDS             36017..36382
                     /locus_tag="M2A35_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(36903..37394)
                     /locus_tag="M2A35_002810"
     CDS             complement(36903..37394)
                     /locus_tag="M2A35_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199090.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFITNIGAGLPDA
                     CAE"
     gene            complement(37375..37888)
                     /locus_tag="M2A35_002811"
                     /pseudo
     CDS             complement(37375..37888)
                     /locus_tag="M2A35_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         9754 a         9893 c         9077 g         9164 t
ORIGIN      
        1 tgtcagtttc ttaagatatc cagaccaacc gccaccaact ggatacgaac aggccgccta
       61 caggcaacac gtaaagatcc aaccaagcca aaatctcctt acctcacaac acggcaagcc
      121 tgcattgcgg cgcttcagtc tccgctgcat actgtccagg tgagcgcggg tgatggcata
      181 acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat atggtatgcc tcgtattcgc
      241 tcccggacgg gaaacgaatt aaggaatctc ttggcacaaa ggacaagcgg caagctcagg
      301 agttgcacga caagcgaaaa gcagaactct ggcgagtaga aaagctaggg gatttacctg
      361 atgtcacttt tgaagaggcc tgcctaagat ggcttgagga aaaagctgat aaaaaatctc
      421 tcgattcaga taaaagccgg attgagttct ggcttgaaca ttttgagggt ataaggctta
      481 aagatatctc ggaggcaaag atttactctg ctgtaagcag aatgcataac agaaagacga
      541 aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa aggtaaagaa ccgcctgttt
      601 atgaaccaaa gccagtatca actcagacaa aggcaaagca tcttgccatg ataaaggcca
      661 ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa agcgcctgtc atcaagatac
      721 cagcagtcag aaacaagcga gtcagatggc tggaaaagga ggaagcaaaa cgccttattg
      781 atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc gctggcaact ggtctgagaa
      841 agtcgaacat cataaatctg gaatggcaac aaatcgacat gcagcgacga gttgcctggg
      901 tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt ggcgctgaac gataccgcct
      961 gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg ggtgtttgta cataccaagg
     1021 cggctaagcg agcagatgga acatcaacgc ctgcggtcag gaagatgcgc atcgacagca
     1081 agacatcatg gctatcagct tgtcgtcgtg caggaattga agatttccgt ttccatgacc
     1141 tcagacacac ctgggcaagc tggctgattc agtcaggcgt accattatca gtgcttcagg
     1201 aaatcggcgg atgggagtcc atagaaatgg ttcgtaggta tgctcacctt gcgcctaatc
     1261 atttgacaga gcacgcgagg aaaatagacg acatttttgg tgataatgtc ccaaatatgt
     1321 cccactctgg aattatggag gatataaaga aggcgtaact gattgaattg taatggcgcg
     1381 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
     1441 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
     1501 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
     1561 atttatttcg ctatagcggc aaacaaacac acaccgctgc gcgtctgaat caagaaaacc
     1621 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
     1681 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
     1741 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
     1801 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
     1861 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
     1921 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
     1981 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatgcc
     2041 gttcagatga tcctctccgg atacacgttt tttcccagcg aaacgcttaa ctatataaaa
     2101 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
     2161 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
     2221 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaagctagg tttgcattca
     2281 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
     2341 atcataaata tcgcatccgc tttcgccaca cctggccgaa caccgctggc taacgctcga
     2401 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
     2461 gccgctccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gatggtttgc
     2521 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
     2581 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
     2641 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
     2701 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     2761 ttaatcgttc ccaaagggtc agctgcccat ccactggaaa tccacagtaa cccgcagaaa
     2821 cagctaaaga gtgctcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     2881 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     2941 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     3001 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     3061 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     3121 cgcagcaaca ttcgtacatt tgaccgcaat gctcttggtt tgcggcatga caccagcagg
     3181 tcgattaccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     3241 aacaatctgc ccggcattta attcgcagtt ttgcggtact tcaacccggc caccaaaact
     3301 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     3361 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     3421 tattaaacga gaatccatca ccccgaatgg aaggttttgc gaaacgttag gatctgttcc
     3481 catcagaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     3541 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     3601 aataaagttg gtgacataac tacggtatgt ataattcacc agcgtaccgg gtggacaaat
     3661 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     3721 attggtgcta ttaaagatat ttgagagatc atagtgttca tcttctgcta cgccagtact
     3781 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca tcgccaataa
     3841 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     3901 cgaaataaca gtgacggctt gctgtaagct ttgcttcggc aatacatagt gcgcaatgca
     3961 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     4021 taaataaact tgcccgtcat cacccacata ctggttattc cgatactgtt ttcccgtacc
     4081 agtgatccaa acggcaccgg ttttccgccc tgggtaacgg taatgagcgc ccgcacgcct
     4141 atgcgggtat caaaattggc acgaaccaac gcgccttgcg tcggcacaac gctgctaata
     4201 tttttttcaa catcgatgga attccccatc gtattggtat caagcgcgat acggttatac
     4261 cgataaaccg tggcatacgg catcaccgca tagccgcgcc agtcggttaa aatgccagtt
     4321 tgattttcaa tgcgtacacc gcctgcgcca ggcgctttaa tcaaaacatt ggtatcccct
     4381 aaaggctggc tcagcgttat gccattttca tgcccgacca caccgccaga cagctgccag
     4441 ttaacgtcat gttgatcgcg gtcgtagtta tacccgaccc ccagcgtagc gtaagcggcc
     4501 tgccagtttg ccgtggcgct gcccgtatac ccattattcg aggtatcacc ctggcttacg
     4561 tgataactca ggttgtggcc ttccagtaag gtcccaccta cacctgccag ccagctattt
     4621 tgcccgttgc tgttacggtt ggcgttaaag gaggcataag cgcggtcgag cgcattttcc
     4681 cgggtgtagc gacgtttggt cagaacattg aaaggcactg aaacattaag tccgacaata
     4741 cgttcgttat cggggatccc tacagattca ttccacgaaa atgagagtga ataactgatg
     4801 ccaacccagc tgctggtata ccccacctga taccaggtat ctgaatccga agtattccag
     4861 tatttttgat gggtaccaga gatataaagc gagccaaagt cattaagtga ttgtgaaata
     4921 tttaactgca aacggtcttt acggctaaag cgtaaattgt ggtaattcac gattatcggt
     4981 tcatcccgat gctcaccgtc gtaatcgtaa tcatattcgt acccctccat tcgacgatac
     5041 gcaacatcat caagggtata aaaaccttgt gtcgaatagc ggtaacccat taactgaaaa
     5101 ttggtgccga aggtgttcat cgatttcgca tagaggaagc gaatggaatc cccctcatga
     5161 cgactgtcgt cggctaactg actgcgcgca tgggtcacat ccagcgacac tgccccccag
     5221 tttccgagat tgcgccccag tcctaataaa aaggcggtgt aattggcaga taattgcgtc
     5281 ccgccgtagg cagtaaattc ctgtggtaaa ccgccgagtg ccgtgccctg aaaaaagaaa
     5341 ggcgatgatt gctgactatt accgctacga aaatcgcccg ccgtcaggtc aaatttgaaa
     5401 cgcccttcgc gttgtaaaat tggcactgtt gaatacggga tagtgtaatt ctgctgatta
     5461 ccatcgcgct cgtcgatggt gacgtccaga tcgccatttg aagatgtcgg gtgcaaatct
     5521 gtaatttcaa aagcgccggg ggaaacatag ctttgataga taataaaacc attttgccga
     5581 atcgttagct gggccgccgt acgggcaatc ccacgtaccg ttggggcaaa cccttgctgg
     5641 ctatcaggat acatattatc ggaagaataa agccgtacac cacgaaatcc aacaccatcg
     5701 aaaatatcac ttcctgtatt gccgtctccc attaccagtt cacttttcag cggaataatg
     5761 gcgcgctgta cccaggtgcc aatattattc cactgttctg aatgatatcc atttccgcga
     5821 aaatagttcc aggaaccatt gttgcgtaaa cgccacgggc caatattaat cccgctgagc
     5881 tcactaaaaa aatagctatc gttaccatta cctctgttac cggtgaaatt ataattcagg
     5941 agtaaagcag gaataccttc atcccactct tcaggcggaa tgtaaccgtg agcgctactc
     6001 aacaacgtta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc aaagttaatt
     6061 gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg ctgggctaac
     6121 tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt aaaacagggc
     6181 attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac gatatcctgt
     6241 gaaccaatga actcatcatt acgccagaga tccacccgat acactcccgc aggttgatga
     6301 ttgcctctct caaagcgcga taaatcagca acggatgtgc cattctctaa taaaaatgcc
     6361 gggttgaaat aactttccgc atagcttaaa aaggagagta aaatggcaag agatgaataa
     6421 caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt gggtattttc
     6481 ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc tctgaccggg
     6541 gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac tcctgagggt
     6601 aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt ttggctgccc
     6661 acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt gatatagtta
     6721 ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg taattgaatt
     6781 ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt cctgccgttc
     6841 aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag agactcacga
     6901 tctgctgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct tttgggttca
     6961 ctaacaaaca gtggcggtgt aatgatgaat gacttttctt ttacaccgct gctgttttca
     7021 atccacgaat tgaccagaaa acgctcattg gtatggctat ttctaatcca taccgcagtc
     7081 tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc tccggcagca
     7141 tgggcgctgg ccgaaatgat taaataaaat ataatgagca tcaataactt tattttagtc
     7201 atcatagtta atacgccttg tgtattcctg acggcaaaaa taagaaatat aaagaaatga
     7261 ttgctttggt ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
     7321 agtgaacatg atttcgattt acattgccac cataccgctt cagacaaccg gtacattact
     7381 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
     7441 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
     7501 cgtgcagaaa aatgaagaac gttggttccg gggatcaagt tctggttggt tgagaagcta
     7561 ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc gacacccgtt
     7621 gctgttgttg tattggtact ggatgcaatg gccaataaat tatcattgat tgcatcagcc
     7681 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
     7741 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
     7801 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
     7861 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
     7921 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
     7981 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
     8041 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
     8101 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa caaaaatata
     8161 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
     8221 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgga
     8281 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
     8341 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
     8401 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
     8461 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
     8521 cgcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
     8581 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
     8641 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgata
     8701 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
     8761 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
     8821 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
     8881 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
     8941 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
     9001 ccactaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
     9061 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
     9121 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
     9181 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
     9241 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
     9301 cacaggatga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
     9361 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
     9421 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
     9481 ggtcagacag tgagttttgc aggtcacagc gtacaggttg ttgcctgatc cctttcccgg
     9541 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
     9601 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
     9661 ccgttcccct atgcattggt ttagggttgg gttcgaaagt catccctccg cgtctgttat
     9721 ttgccggaat catcctggcg atgctgcccg acgccgacgt attgtcgttt aaatttggcg
     9781 ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg tttgcgtttg
     9841 ttgtcccgct gttatgtgtg ctgattggac gccgatggtt cagggctggg ctgattcgct
     9901 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt gctggattcg gtaaccactg
     9961 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
    10021 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacgccgtac gggcatcagg
    10081 tgataatttc tgaattgatg tgggtgtggt tgccggggat ggtgcttatg ggaatgttgt
    10141 ggtggcgcag gcgataaccg gatgcgaaaa ctcgcatccg gcaacagcgc aattacttac
    10201 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
    10261 cacgcgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
    10321 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
    10381 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
    10441 ttgcattcgc cgctgccata tcttctgctt tcagacggtt tacttcacgc gccatatcaa
    10501 acagtacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
    10561 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
    10621 agagacgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcaactggc
    10681 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
    10741 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
    10801 ccatccccga gtgcatccag tagttcacat actggccatc atgggcacag gtggactgcg
    10861 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
    10921 ggttacccag ctgcttgcag ttcattgccg aacattcaat gtgccagcct ggacggcccg
    10981 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
    11041 ccatcgggtt gcgtttgtcg tcgaccacgt caacgcgcgc acctgcctgc agctggtcga
    11101 gatcctgacg cgacagcacg ccatatgtcg gatcggtcgg gacgtcgaac atcacgtcgc
    11161 cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
    11221 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
    11281 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
    11341 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
    11401 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
    11461 gaccgatatg acagagatcg taaacggtga ttccacacac atacatgccg acttccccgg
    11521 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
    11581 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
    11641 cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat
    11701 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
    11761 ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca
    11821 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
    11881 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
    11941 accaaagaac cgatcaaaaa cgaagccaac aacggtctga aaaatacccg tggtacgctg
    12001 gcaatggccc gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
    12061 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
    12121 gctgaagtgg ttgaaggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
    12181 agcggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
    12241 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
    12301 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgaa gcgctgtata
    12361 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
    12421 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
    12481 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
    12541 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc
    12601 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
    12661 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgag
    12721 cgaacagtaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
    12781 cggtagtcag tgcgatggaa aaacatcagg tgcaatggct gatccacgga catacccatc
    12841 gcccggcggt acatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactcggtg
    12901 cctggcatac ggaaggttcg atggtgaaag tcacggcgga tgacgttgag ctgattcatt
    12961 ttccgtttta aaaaacccgc aactttgctg atttcacagc cacgcaaccg ttttccttgc
    13021 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
    13081 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
    13141 gcgactgggc taccatgcag ttcgccgccg aaatcttcga aatcctgaat gtcccgcacc
    13201 acgttgaagt ggtttctgct caccgcaccc ccgataagct gttcagcttc gccgaaagcg
    13261 ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgcctg
    13321 gtatgattgc cgccaaaacg ctggtgccag tgctgggcgt gccagtacag agcgccgcac
    13381 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
    13441 cgctggcgat tggtaaagct ggcgcggcaa acgcggcgtt actggcagca caaattcttg
    13501 cgactcatga taaagaactg caccagcgtc tgaatgactg gcgcaaagcc cagaccgacg
    13561 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
    13621 cgggcagtta ggccgtatgc tgcgccaggc aggtgaaccg ttaggcattg ctgtctggcc
    13681 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
    13741 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
    13801 gaaccgcgat gtgttcccga ttattgccga ccgtctgact cagaagcagc ttttcgataa
    13861 gctccacctg ccgaccgcac cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
    13921 gtttgagcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
    13981 cggtcaatgg cgtttacgtg ccaatgaaac cgaacagtta ccggcagagt gttacggcga
    14041 atgtattgtc gagcagggca ttaacttctc tggtgaagtg tcgctggttg gcgcacgcgg
    14101 atttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
    14161 gcgcaccagc gtcgcttttc cgcaggccaa tgcgcagcag caagcgcaag ccgaagagat
    14221 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
    14281 caccccgcaa ggtctgctga tcaacgaact ggcaccgcgt gtgcataaca gcggtcactg
    14341 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
    14401 gccgttaccg caaccggtag tgaatagtcc gtcggtgatg atcaacctga ttggtagcga
    14461 tgtgaattat gactggctga aactgccgct ggtgcatctg cactggtacg acaaagaagt
    14521 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
    14581 cgcgacgctg gaagccttga tcccgctgct gccgccggag tatgccagcg gcgtgatgtg
    14641 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
    14701 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
    14761 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
    14821 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacca
    14881 cggcttctga cataaccact taccgccgtt accttcggcc ccatcgaacc ataggctttg
    14941 gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cgcgttgctg cggcgttccc
    15001 cagttttcat aaaccgcgtc agcatcggtg aggatcacca gtccatcggc attaatctgc
    15061 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
    15121 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
    15181 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
    15241 aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc cttttgttct
    15301 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
    15361 gaaacctcaa tgcgcgtcag caccgtcgtg acaggcggca tctgcggctg tgcgctcaaa
    15421 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagcac atccagcgga
    15481 tacggatcta cctctttcca cgccagattc tgcaatgcca gcagccccac ctgcggccca
    15541 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gcgccagcgc gggtacagca
    15601 ctggcgatat tgcgatattg attttctgcc gtcagcgcct caccgcgctg gagtaaggcg
    15661 ttgcccccaa gagctacaac cagtgttttc atggtgttcc ttgtaataat tgttgaccaa
    15721 gccagaaccc cagcagcgta tctgcccctg acgtgtgccc cagcgccagc agcgaatcaa
    15781 tcgcaacagc ggtacgtttc gggcaactca gagcatgaac aaagtgcagg agtggcgagg
    15841 cgaaatatcc ttgcgcggcg tagcgtaaat aactgacgct caccgctgtg gtaacgagtt
    15901 gaagattgtc ggagcaggca aaaaactggc gaccggagcg cgcatctaaa gcgccataat
    15961 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc
    16021 ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    16081 gacgaaactg gcgtaattca gcggtcagcg gatcgcttgc cgccatcgcc agtggtccga
    16141 aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgtgaag
    16201 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    16261 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    16321 cgaattgcgc gcggcgaagc acccatcctc cggggccgaa accactaccc tgacgatgca
    16381 gcgttaacag ttcgccgctt tccgtcatca ggttaatcgc ccgtcgccac actcccgcta
    16441 accgccagga ctggcgataa ttcggtgcac tacggctggc aagcagagga tggatgatcg
    16501 tcattgcgcg ttcctcaacc aatccccatg ctttcagcca gtgcttccag cgcctgttca
    16561 aaacacgcca gcggtgcccg cacagtgcct gcgccaatct gcccgatccc cgcctcttta
    16621 tgggcgatac cggtattgat gagtggcgta atgccggttt ctaccacgcg acgaatgtcc
    16681 agtcccaggc acgcgccctg aaaatcccag cctgggatct gcaactgcat attgcgctca
    16741 aggtaaattt ccgccatctc ttcagatacc gctctggccg cttccatgcc acccgcaccg
    16801 acaaagcgcg ttacgccagg cgctgcgatc attgccgcac ctccgatacc aaaggtttcg
    16861 gtaatcgcgc tatcgcccat atccgggttc gcctgctcct gcgagaagcc ggtgaaaaac
    16921 agaccttgcg gagtgtttac aggcgcagta aaccagcgtt cgcccagccc gcttacccga
    16981 atcccgaaca tattgccgtt gcgggtcatt gccgtgacga tgctgcctgc gcggatcatc
    17041 gcgccagcat ccatcgccgc cttgcagtaa gccatcgcga ggttgaggaa gaactgatcg
    17101 gtcacgctga ggaaatccat cacttcggcg atgtgctgtt tatcatgatc gaggcgagca
    17161 atttgtgggg ccagcgcacg cattaacagt gcagaggaag caatattgcg ttgatggaac
    17221 tcatcgccca tcgtaatgcc ctgcgccatc atcgccgtga gatcgatacc gcgctccatg
    17281 cgccccagcg ccgcgcttaa tactggcatt aacacatcgc gcatccagcg atggcgagtc
    17341 aggacatctt cgccgtaagc gccaaaacgc atcactttgc cgataccttc gttgaggttg
    17401 cagtacgccc ggttgccgtc ggtcacgttc tcgaccacca gcatcggcat actggcagaa
    17461 gtaatgccgc ccattggccc gacggcattc acatggtgac aaggaatgaa gttcacttcc
    17521 ccctgctcca gtattgccag cgcctgcgct tcatctttcg cccaaccttc gaacagacat
    17581 gcgcccacgc acgccccttt catgggtccg gtcatctcct gccagcgcat tggcggcccg
    17641 gcgtgaagca gtgttttgcc ctcgtttagt tcgctgataa gtgaagaagc cggttgcaca
    17701 tccagccagt gtggacgagc gcgacgaatt tgttcgataa ccgcagcatt ggcttgcgcc
    17761 actgatgtaa acatgggaac cccttattgc aaacgttcta ataaacgagc cagtttttta
    17821 ttgccgccgg cgactggcga ccattggtaa tgcacaaccg gtttgctggc gctttgtagc
    17881 tccagcgcaa agctgcgtaa tccgatgtta atcacggcga cgttttccag taatgacggt
    17941 gtgtgttgct gagtggcagg cgaaagcgga cgaatcagtg ccgccgccag caaggtggct
    18001 tccggcagcg aactcactac cgcgatccct gcatcttcca gcgtggcgat ttgctgcgag
    18061 cggcattgcg ggtcacgttc agtacctgtc accgtggcaa tggcatacag tggttgattg
    18121 tctgaacgcg cggcacaggc tttttgccag gcgcttacca gcgaggcggc aggatcggcg
    18181 gtcgcaccga agccaatcac gacatcaagc agcaatacgc gcacttgcgg tttagcgccg
    18241 agatcggcaa ttaactgatt gcgtaaggct gggtcgatca tcggatgggg acgcccgacg
    18301 gtgtagaaat catcgccgag gtcaataatc tggtgaccat cggcgtcgag catcatgccg
    18361 tgatgatggg tatcgtcggc ttccacgcca aggtgcccgg caagtaatcc cgccgcttcg
    18421 gcagccagcg taccgccggt atacaaaccg caaataaatc cgctgctggc aggcgttatc
    18481 gcgttacgtc gcgccgtaac gcgtgaaagc aggcaagcca ggcgtgcggc ctcatccagc
    18541 gaggaggcaa accagacatt ctcgtcgcag gccaccgccg gggtataacc taaaaacagc
    18601 gccaccgtcg gtttgccggt tgctttcatg gcattaacaa ttttcagacg cacagcttcg
    18661 gcaggcggtt ttgaaacaaa tgccagcact tcgcttttct cgtctgcact gagcatttcc
    18721 agcgccgtca gcgcactgat gccgcccact tcacggctga ggtcgcgccc gccaaggcca
    18781 atcgcgtgag taattccctc ccctgccagc gcaatctgtg agcacagctc ctgaatcccg
    18841 gtaccggaag cgccaatgac gccaatgttg ccttccggca tcacgttagc aaaagccagc
    18901 ggcgtgccgg caatcatcga cgtaccgcag tccggcccca tcaccagcaa gcctttttct
    18961 cgcgcgcggg ttttaagttg gatttcatct tccagcgtga cgttatcgga gaacatcatc
    19021 acgttgaggt tgcgatccag cgcctgattc gccagctccg ccgcatactc gccagccact
    19081 gaaatcagcg ccagactggc atcggataat ttctgacagg cactgtccca gcgacgcacc
    19141 tgcgtcaagg actggctgct gcctgacccc tgcgccagtt gttttagcgc ctcttcaagc
    19201 tgctgcataa tcgcctgcgc gatccccgca tccgccgctt cgctacgaat tgccacgcaa
    19261 atatcgttcg gcgtggcgtt attaaaatcg tcatgccaga aacctgtggt atctaataac
    19321 gctttattgg cgggcgtacc catcattacg gaaacatcat caacattttc tgattcgctg
    19381 agttttcgtg aaataatcat taaactgacc gaatcctgaa aacacccttt tttaataaag
    19441 gcgtggatca taacaactcc ttaaaaattg cacccatggc aattgcacga gcagtgttaa
    19501 tgtggcgatt acggtatcga tgacaacggg taaataaggt gatgaatatc acacagggat
    19561 tattaacaaa agaattcaaa taaccgccat gctattacgt gttatttttt aataagcgtg
    19621 gttgttttaa aaaccaggtt aaaagcgagc ggattatcaa ataaattcta actatatttt
    19681 tttgcctgtc tggatcacat aatccagata ttttccctgt atgttaattg cagtcatgct
    19741 tcacaccgtc gttaaaaagg aagacagatg aaaatcagtc gggaaacact ccaccagcta
    19801 attgagaata aactctgcca ggctgggtta aaacgtgagc acgctgcaac cgtggctgaa
    19861 gtattggttt acgccgatgc cagagggatc cactctcatg gcgcggtgcg cgtggaatac
    19921 tacgcggaac gcatttcaaa aggcggcacc aaccgcgaac cagagtttcg tcttgaggaa
    19981 accgggccgt gctcggcaat tttacatgcc gacaatgccg ccggacaggt cgcggcgaaa
    20041 atgggtatgg aacatgccat caaaaccgcc cagcaaaatg gcgttgcggt ggtcggtatc
    20101 agccggatgg gtcacagcgg cgcaatctct tattttgtgc agcaggcagc ccgcgccgga
    20161 ttcattggca tttcgatgtg ccagtccgat ccaatggtgg tgccgtttgg cggcgcggaa
    20221 atttactacg gtactaaccc cctggccttt gccgcgccgg gagaaggcga cgagatcctt
    20281 acctttgata tggcgactac cgtacaggca tggggaaaag tgctcgacgc ccgctcgcgt
    20341 aatatgtcta tcccggatac ctgggcggtc gataaaaacg gtgcaccaac aaccgatccg
    20401 ttcgcggtac atgctctgct ccccgccgcc gggccaaaag ggtatggcct gatgatgatg
    20461 attgacgtcc tctcaggcgt cttactcggc ttaccgttcg ggcgacaggt tagttcgatg
    20521 tatgacgatt tacacgcagg gcgtaatttg gggcaattac atatagttat taatccgaac
    20581 tttttctcct ccagcgaatt attccgtcaa catcttagcc agaccatgcg cgaattaaat
    20641 gccattaccc ccgcgcccgg ttttaaccaa gtttattatc ccggacagga tcaggatatt
    20701 aaacaacgca aagccgccgt cgaaggcatc gaaattgttg atgatattta ccagtattta
    20761 atttccgacg cgctttataa cacgtcatac gaaacgaaaa atccctttgc gcaataatta
    20821 ttgagacagg actttcttat gattacacat tttcgtcaag ccatagaaga aacgctgccc
    20881 tggctttcct cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg
    20941 gaatggctgg aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca
    21001 cgtttcgatg aagtagggaa tttatacggt cgcctgaatg gtaccgaata tccacaggaa
    21061 gtgattctga gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa
    21121 ttcggcgcgc tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcgccg
    21181 ctacgtacgg tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc
    21241 ttctggggca gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt
    21301 gatgccaaag gaaatagttt tgtcgatgca atgaaggctt gcggatttac tcttccgaac
    21361 gccccgctaa ctccgcgtca ggatattaaa gcctttgtcg aactgcatat cgaacagggc
    21421 tgtgtgctgg aaagtaatgg gcagtcaatt ggcgtggtga atgcaattgt cgggcaacgt
    21481 cgctatacgg tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat
    21541 cgtcgtgata cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa
    21601 aagatgggcg atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta
    21661 aatgtggtgc cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg
    21721 ctgcgtgatt tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac
    21781 atttgtattg atatcgattt atggatggac gaagaacccg tgccgatgaa taaggacctg
    21841 gtcgccaccc tgacagaatt gtgtgaaagt gaaaaactga attactgggt gatgcacagt
    21901 ggtgccgggc acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc
    21961 cccagcatca acgggatcag ccataacccg gcggaacgca ccaatattac cgaccttgcc
    22021 gaaggggtca aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc
    22081 ataaatggga tatttaaata acgtcaccgg ttaccgcgat gatttactgg ctaaccgtgc
    22141 gattgttaaa cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat
    22201 tccgggcttt gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt
    22261 tgttgattat ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga
    22321 aggcattgaa acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa
    22381 aacatttgcc ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt
    22441 tgttaacgcc caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt
    22501 agaaggccat gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta
    22561 tgaaggtatg gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat
    22621 gaacatgcat atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacatgt
    22681 tcaggaacac ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg
    22741 gatcccggtg aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta
    22801 tggcgtaggg cgtggtgaag cgttcagcta tatttactcg aaagattgta accgcgacgt
    22861 cgagatttaa gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt
    22921 ttataccgtg gctttttttg tagtaaattc aggtaaaaaa agatgcgata taaatcgcat
    22981 ccgctttaaa ggtaagggtc agtttttaat tccctgacct attttaatgg cgcaggcaat
    23041 atttcgtgcg ctgttgaaga gattggtttc accgctggcg agcacttcgg ctaaaggtgc
    23101 cagacgtggc aaaatgctga ataccgcatc aatgccgtac tggtgcacca cttccacgcc
    23161 atcacccaat acgccagcaa tcccaatcac cgggacatta aactgcttcg ccaccgacgc
    23221 cacacccagc ggcgctttac cgcctgccgt ttgcgagtcg atgcgccctt ccccggtaat
    23281 caccagtgct gcgccctgca ctgcctgcgc aagattgacc gcattcaaca caatctcaat
    23341 acctggttta atatccgcat tgagaaatac cgccgccgca atacccatac cgcccgcagc
    23401 gccgccgccc gccatctggc agacgttgat ttcagtttgc tgttgtaaaa cacgggcgta
    23461 attttgcagc ccctgttcca gctcttcgac catttccggc gttgcccctt tttgcgggcc
    23521 aaacaccgcc gccgcgccgc gtgcccctac cagcgggtta tcaacgtcac atgctacttc
    23581 aatatggcag ttcgccagac gcggatcgca ttcctccatc tcaatgcttg ccacgcgcgc
    23641 taaattgccg ccgttagctg ccagcacctg accgtcggca tcaaggaaac gcacaccgag
    23701 cgcctgcgcc atgcccatac caccgtcgac cgtcgcactg ccgccaatgc cgagaataat
    23761 atggcgaatg ccgttatcca gcgcatgacg aattaactcc cccgtaccaa aactggaggc
    23821 cagcaacgga ttacgctttt caggcgcgac cagcatcagg ccacttgctg ccgccatctc
    23881 aatcaccgcc gttttcccgt cgccggtaag gccataaaaa gcattcactt tttcgcccat
    23941 cggcccgcag acttcaagcg tcacgatgtt gccgcccgtc gcggcgacca tcgcatccac
    24001 cgttccttcg ccgccatccg ctatcggcaa acagatatag ttcgcatcgg gaaagagggt
    24061 cgaaaacccg gctttaattg cctgacaaca tttttctgca cttaagctct ctttaaaaga
    24121 gtctggcgca atgacaatct tcattatcaa tcctttttca acaaattatt cgatacgttc
    24181 ccacggaata aggttatcca taagaacagc cagtaaaatc ccgaccaata agccgttact
    24241 gagcagagga cgaagcgtta atggaaggtc ttgcagatac acaggcggta atgccattaa
    24301 aaatatgccg acaaataacg gcaatgcgag tcgataaata ttgcgagcgg taaacgttat
    24361 ttgctggcta aacaccagcg cggaaaagag taaaggcaga taagaaacca gcatgaccgc
    24421 gctactcacg ggtaaaggga tactgcaaaa cagtcgcgtg agtgccggaa ccagcgccac
    24481 cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc ctgtctgggt
    24541 taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg gtacggttat
    24601 cagcgtcata aatccggtcg ccacaaagct gcgacgataa cgcgtatttc ccgcgccctg
    24661 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattcaccag
    24721 acctgtaatc accgccgtca gaataattcc cggagacaaa gcacctccac tgccgagcgg
    24781 gaaccactgc cagtgcaact caccggagag cgagtgcgaa gaaggaaagc aaaagtacca
    24841 caacaaccag ccggttatgg tgccgaccag caggccataa cgggcaaaac gttgcggcag
    24901 gaagataatc atccccagta ccaggcacat caccgccacc gagagcgcga acggcggtaa
    24961 ctgaatttta aaatttgggt cggctatgcc aaacggcagc ccgagcatac ctttgaaaaa
    25021 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
    25081 taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca gcacgccgga
    25141 gagcgcaatc cccaccgcca ggctggtagc gatatcgttg atcggtgtcc cgcgcgatgc
    25201 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacccggcc cttccataat
    25261 agcgcgacga tgaccgcaga acgcctgagc gaagcaggcc agtgcggtag caagaaaagc
    25321 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
    25381 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
    25441 ttcgcggctc actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
    25501 ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
    25561 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcgtg
    25621 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
    25681 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
    25741 ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt cgccattaat
    25801 ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa catcacgtcc
    25861 atacagtttt gcagaccggc aataccgccc catgcttcca tgatgttgcc ggctttcatt
    25921 tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
    25981 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt cgccagagtg
    26041 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaagta
    26101 acgtcctgac cttcctgacg tgcacgagtc acttcttcaa cgccttccgg gctgctgata
    26161 tggcaaacgt gcagacggca accggcaact ttcgccagat acagcacgcg gcgaatcgct
    26221 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
    26281 cgtttcgctt cttcgcccag tgcgtcacag atcagcgcgt tttcgcagtg caccagcacc
    26341 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
    26401 cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt gaagccgaca
    26461 acgcccactt catccagctc atgcagacgg tcgatgttgt aagacaccag gccaccgagt
    26521 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagcg
    26581 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
    26641 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
    26701 atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc atccataact
    26761 tcttttgcat cgcccagatc ctgaccgata gcagcaattt tcccgccttt aacggcgata
    26821 tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat taaatcaaaa
    26881 gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc aatatcggga
    26941 ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt ttctttaata
    27001 aggcgtaggc cgcaaaggcg acgatgacgc cgacaaacca tgaaacacgc gataacggtt
    27061 ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga gttactgaaa
    27121 acgcggtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg gtgtacagtt
    27181 catcaagatt aatttgccca cgcatcacca caaaataatg tgccatcatg acaccaatta
    27241 ccggacccag cattccgccg atgatatcga ggaacagata aatactgtcc tgattttcca
    27301 ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg ccgtttttat
    27361 aggtcagttt tgtcggtgca atggcagcaa tctgataacc ggccggaata atattaccgg
    27421 tggcgttagt ggaaatagtc gtcatcagaa taaccagtac cgcaaagaac gaggcaaaca
    27481 ggctgtccca acgctgaaca atatccagca cgttccaggt gtcagcgccg tagtgaatac
    27541 tggctccggc aataatacat accccagcga ccgcaaacag aatataggcc acaactaaac
    27601 ccagcgtttg ccccagcgct tgctcacgaa acgagtgggc gttttgcgta aagtcggatg
    27661 cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt aatcaccacc aggaacaaga
    27721 atccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt ggaccgagcc
    27781 cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg cacgggttaa
    27841 gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca acgttgacca
    27901 gccagaagag taagaaagta attaagcccg gtagagaaag ccctaacagc gtgaaatcac
    27961 caccgagagt taaaaatccc ggccagattt tgccaatcag aatcaagcag gccagtgacc
    28021 ccgcataaca ttgcaggcca aaccacataa tggcagcaat accgcctctt aataatccgg
    28081 gaaacagtgc gccacgtacg ccataagaag cacgcaggat catggcaaac ggtacgccgt
    28141 atttactgcc cgcagcgccg tttaatacca ttaccgcggc aatgaaaaag gcgctgagga
    28201 taattgccag cataatacta aaggttgaca agccgagaat aaaaaagccg ccgaccatca
    28261 cataattggg aacgttatga accgaaccca tccataaggt aaaatagtta aatgttttcc
    28321 aggttcgctg gctttgcgtt ttcggcaata gatcttcgct atagccgcgt tgctggaata
    28381 gttttctctg atgttccata aatccctctt aatttcacgt atcgaataac gttgttttaa
    28441 tctagctatg aggaacaaat aaaataaaaa tattattttt aatgctgaag caacatcttt
    28501 ggctgctttc tttttttaat attgctggcc gattcatttt ctgatatcgg ccatttttat
    28561 tgcgggtatc aggccagttt atggttagcc attaattcca gcgcctgcac taacgcagag
    28621 tgatccaact ggctgccacc gtttgccgcg caggtattaa ataactcctg gcaggtcgca
    28681 gtgtttggca ggttcagcgc tagcgctttc gcactttgca gtgccaggtt gagatctttc
    28741 tggtgcagcg caattttgaa acctggatta aaagtgcgtt taatcatgcg ctcaccgtga
    28801 acttccagaa tacgtgagga agcaaagccg cccatcagcg cctggcgcac acgtaccggg
    28861 tccgcaccgg cttttgaagc aaatagcagg gcttcagaaa ccgcttcaat attgagcgcc
    28921 acaataatct gatttgccac tttgcaggtt tgaccatcgc cgttaccgcc cacgagggtg
    28981 atatttttac cgagcagttc aaacagcggt ttaacacgtt caaataccgc ttcatcaccg
    29041 ccaaccataa tcgacaacgt cccttcacgc gcaccgattt caccgccgga gactggcgca
    29101 tcgagataat cgccgcccag ttcattcacc tgacgagcga aacgcttagt ttcaatcggg
    29161 gaaatggagc tcatatcaac aatggttttg cccttcagcg aggctttggt acaaccattt
    29221 tcaccgaaca gaacttcttc aacctgtggt gtatctggca ccataataaa aatgatgtcc
    29281 gatgcttccg ttacctggcg cgcagtttca acactgacgg cacccagtga cagtaattca
    29341 tcagcaaccg gaccaatggt tgtgacatgc agttgatgac cggcacgcgc cagattaatg
    29401 gccatcggtg tacccataat gcctaagcca ataaatccca gtttcataat taacctcttt
    29461 taaatttcgc ttttcctgaa ttcagacaac acgatcccct gccaacttag cggcctaaaa
    29521 aagcattgcc tgaacagcgt tacgttttaa cggtacggat ccatccagcg taaaccggct
    29581 tccgtggtgg tttggggtgt atattcacac ccaacccaac cgttgtagtc agaattttcg
    29641 attaccttaa agagataatc ataattaatt tctccggtcc ccggttcgcc gcgatgcgga
    29701 ttatcagcaa tttgcaggtg accaatttta tcggcccact gagtcatggt gttggttaat
    29761 tcaccttcca tccgctgcat gtgataaatg tcatactgaa ttttcaaatt gcagcaacca
    29821 acatcatcaa tcaatttcag cgcctgccga gttccggtga gatggaaacc aggaatatca
    29881 aaatggttaa taggttcaat cagtaataaa atatcttctt tcatcagcat attcgcggca
    29941 taacgcaggt tttctacaag cgttgcgtga atctgttcac tgctgaaacc agccggcgtt
    30001 ttaccgacca gacagttaat ttttttatta cccagcgcac gggcataacg aatcgctgct
    30061 gctacgccat cccgaaactc ttcttcacgg ccaggaatac aggcaatacc gcgctccccc
    30121 gccgcccagt cacctgccgg taaattgtgc agcgtatgtt cgagtttatt actcgccagc
    30181 acctgtttta attcttcaat gtcgtagtca taaggaaaca taaattcaac gccgcgaaaa
    30241 ccacactgcg cagctttctc aaaacgggcg agaaaatcat attcaccaaa taacatcgat
    30301 aaattagcag agaaacgtaa cattatttat ctcccttatt catagtgcat gaagcaggtt
    30361 tcagtcggtg catccgctgc gttatcggcg atatcttcaa attccatgac gttatccagt
    30421 tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa cttccacgac taccggtacc
    30481 cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc ttccggtttg
    30541 aagacgcgaa tagctttaca acctaaacct tccgctactt ttacgtggtc aacaccgtag
    30601 ccattcactt cactggagtt gatattctcg aaagcgagtt gcacgcagta gtccatatca
    30661 aacgcgcgct gcgactggcg aatcagcccc agataagcgt tattgaccag tacatggatg
    30721 tacggaatgt tgaactgtgc gccaacggcc aattcttcaa tcaggaactg gaagtcgaag
    30781 tcaccggaaa tcgccaccac tttgcgctct ggatcagcgg cacaaacccc cagcgccgcc
    30841 ggaatggtcc agcccagcgg gcctgcctgc ccacagttga tccagtggcg gtctttaaag
    30901 acatgcagca tttgcgcagc ggcaatttgc gacagaccaa tggtggtgac atagcaaaca
    30961 tcacgtccaa aagctttgtt catctcttca tacacacgct gcggtttaac cggaacgttg
    31021 tcgaagtgtg ttttacgcaa caaagtacgt ttgcgctgct ggcactcagc aacccactct
    31081 ttacggcatg gcagacgccc tgctttttgc atttcctgcg ccacttcaac cagcagtgtc
    31141 agcgccgctt tagcatcaga gacaatcccc agatccggac acagcacgcg gccaatttgc
    31201 gtcggctcaa tatcgatatg aacgattttg cgcccttcgg tgtatttctc taccgaaccg
    31261 gtatgacggt tagcaaaacg gttaccgata ccaaacacca tgtcggacgc cagcaacgtt
    31321 gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc cggccatcag ttcatgatcg
    31381 tccgggatac agccccagcc catcagcgtt gggatcaccg gaacgctggt cagttcagca
    31441 aactgttgta acagtgcagc tgcgtcagca ttaattaccc cgcccccggc aacaatcacc
    31501 ggacgtttgg cctggattaa catttctaca gctttttcga tctgcatacg gctggcagca
    31561 agtttgtaga ccggcagcgg ttcgtacata tcaggatcaa actcgatttc cgcaacctga
    31621 acgtcgaacg gtaaatccac cagtaccggt cccggacgac cagaacgcat caggtgaaat
    31681 gcctgttgca gcacacgagg caccagcgcc gcttcacgta ctgtaaccgc cattttgctg
    31741 accggtttag caattgcttc aatatctacg gcctgaaaat cttctttatg cagacgggcg
    31801 cgcggtgcct ggccggtaat gcacagaata ggaatggaat cagcagaagc ggaatagagc
    31861 gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc gatattccct
    31921 gccgttgcgc gggtataacc ttccgccatg tgcgaagcac cttccacatg acgcgccaga
    31981 atgtgacgaa taccgccgtg cttacgcatc gctgagtaga acggattgat tgcagctccc
    32041 ggaacaccga aggcggtggt gataccttct ttctccagca catacattgc cgcgtcaacg
    32101 gctctcattt ttgccatttt attcctacct ctatttattg gaaaattttt ccaactttca
    32161 aattttgtca aaaaaacccg ctccgcagag cgggatgcga cagacattat ggatgtgctt
    32221 tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct gaccagctca ccctgactga
    32281 caaaacgatc ttctgtcagt cttgatgaag gcccggagat ggagatagcg gcaacaacgc
    32341 taccgacatc atcgtaaatt gctgaagcta tgcaattcag acctacaaca tgctcttctt
    32401 tatctacggt atagcccagt tcacgcgctt gttccaggtc cttcagcaag gtgggcatat
    32461 ccacaagcgt agttggcgta aactgctgca aaccggtttg cagaatgatg ctcatcaact
    32521 cctcttccgc cagcggataa agcaacgcct tgcccgcacc ggaagcatgc agcggcagac
    32581 gactgcccag tggcgcacac atcctgacca ttgatttaca ctctaactga ccaattaata
    32641 ctgcttcatt gccgttacgg atcgcgacat tgaccgtttc gccggaaagt aacatcaggc
    32701 ggcgcataaa cggcccggcg acggagagga catcgcggtt atggatgtac gccgcaccga
    32761 cgttaaagac acctaatcct atatgccacc agcctaattg actgtcctga tagacaaaat
    32821 ccgctgcttg taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa tccagattga
    32881 gagaaatatc gctaaccgac gaacttcccc cacttttttc cagatattgc agaatcgcaa
    32941 ttccccgctc taacgcctgt gcgcccttct gtgccacagg ctccgcctgt cctggcctgc
    33001 cgcgccgtct aacttccgtc atacctacgt taactccgac ctacaagtga gcataaacaa
    33061 tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac agagttcctg
    33121 ttcaggaata ctttcaacat cacagttgtc actgccgccg cgatcgatgg tcagaaaatc
    33181 ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg ttacgatggt aattcactcc
    33241 ctgttcgccg ttggtgataa acgcccgcag cgttgacagg tctggtttgt cgtcgcctaa
    33301 cgccacgacc accacaaaca cttcaccttt catcgggata aaggcctgag tgcccagcgg
    33361 atgacgttcg agttcgtaaa tggtcagcgg cagattcgcc ggttgcgcgc ggttaatgct
    33421 gataagcgta cggtcttgct caagaatctc aaccagcgcc aaatcgtggt aacgctccac
    33481 caggccattg ttaatatgga aaaaatcccg ttgctgagtt tcgattacgt cgccataagc
    33541 actaaaggct tcctgactta acggtaatac ctgaagtttc atctttttgt ccttgtaaca
    33601 taattccgtt caaccatcgc cgaccggtca gcttggtttc aataacaggc aatctaaaac
    33661 cttaagagaa caacatcaat acaaaagttg gaatattttt ccaaaactag atttagtcca
    33721 caaattgaac agggttatca tgtaaaaata ctgataaatc agaggataag aaaatcacgc
    33781 aacttgcgga tttaaaaggg gatacctgaa gatgttcgat ccagaaacct tgcggacttt
    33841 cattgcggtt gctgaaacag gaagtttttc aaaagcggca gaacgattat gtaaaaccac
    33901 ggcgacgatc agttatcgca ttaaacttct ggaagagaat accggagtag cgctgttttt
    33961 ccgcacgact cgcagcgtga cgttgacagc ggctggcgag catctacttt cccaggccag
    34021 agactggctg agctggctgg aaagtatgcc aagcgagctg caacaggtga atgatggcgt
    34081 ggaacgccag gtgaatattg tcatcaacaa cctgctctac cacccccagg ccgtcgccca
    34141 gttgctggcg tggctgaatg aacgttaccc ctttacccag tttcacatct cccgacaaat
    34201 ctatatgggc gtctgggatt cgctattata cgaaggtttt tcactggcta tcggcgtcac
    34261 gggaaccgag gcgctggcaa atacctttag tcttgatccc ttaggatcgg tgcaatggcg
    34321 ctttgtcatg gcggcggatc atccgctggc gaacgttgaa gagccgctaa cagaagcgca
    34381 gttgcggcgc tttccggcgg tcaatattga agacagcgcc cgcaccttaa ccaaacgcgt
    34441 cgcctggcga ttgcctgggc aaaaagagat tattgttcct gatatggaaa cgaaaatcgc
    34501 cgcccatctt gcgggcgttg gcattggttt tttgccaaaa tcgctttgcc agtcaatgat
    34561 cgataatcaa caactagtta gccgggtaat cccaacgatg cgccctcctt cgccattgag
    34621 tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa gatattgtga ccttgtttac
    34681 ccagcgcagg ccggaaatca gcggattttt agaaattttc ggcaacccac gcagttaaaa
    34741 atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg aggttcactt
    34801 ttaactatgc aagagagaca cacggaacag gactatcgtg ccctgctgat tgctgatacg
    34861 cccattattg atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc cgccgctatc
    34921 aatctgccgt taatgaataa cgatgaacgc gccgccgttg gcacctgcta taaacagcaa
    34981 ggctcagacg cagcgctggc gctggggcat aaactggtgg cgggtgaaat tcgtcagcag
    35041 cgcatggacg cctggcgggc agcgtgcctg caaaatccgc aaggtattct ctgctgcgcc
    35101 cgtggcggtc agcgctcaca tattgtgcaa agctggttgt atgcagcggg gattgattat
    35161 ccgctggtgg aaggcggtta taaggcactg cgccagaccg cgattcaggc gactattgaa
    35221 ctggcacaaa aaccgatagt gctgattggc ggttgtactg gcagcggtaa aacgctgtta
    35281 gtgcagcaac agccgaacgg tgttgatctg gaagggctgg cgcgccatcg cggttcggcg
    35341 tttggtcgca cgttacaacc acaacttagc caggcgagtt ttgaaaacct gctggctgcc
    35401 gaaatgctga aaaccgacgc ccgtcaggat ttgcgcctgt gggtgctgga agacgaaagc
    35461 cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg agcgaatgac tcaggcggcg
    35521 attgcggtgg tagaagatcc gtttgagatc cgtcttgagc gcctgaacga agagtatttc
    35581 ttgcgtatgc atcatgattt tacccacgct tatggcgacg aacagggctg gcaggaatat
    35641 tgcgaatacc tgcatcacgg actttcggcg attaagcgtc ggctggggtt acagcgctat
    35701 aacgaactcg ctgcccagct ggatacggcg ctgacaacgc aactcaccac cggcagcacc
    35761 gacggtcatc tggcctggct ggtgccgtta cttaaagaat attacgaccc aatgtatcgc
    35821 tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc ggaagtggcg
    35881 gaatgggtta aggcgcggta atgagggacg tatttgtatt agtcgcgact tgatctgaca
    35941 catggccttg aaaggttgag agttaccggt tttgatatga gtgtcgaatc cttatccaaa
    36001 acaagaggta actctcatgc ttcatactgc caatcccgtc atcaaacaca aagccggttt
    36061 gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc tgtaaaatca tgggcgtttc
    36121 gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa ggcggtgtgg atgcgcagat
    36181 taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc gatgaggcaa ctgaacaggc
    36241 tgttgttgat tacgccgtcg ctttcccgac acatggtcag caccgggcca gcaacgagct
    36301 gcgtaaacag ggggttttta tctccgacag tggtgtccgt tctgtctggt tactccataa
    36361 ccttgagaat ctcaaaagac gatactgaac aaattttatc aggtgacgtt ccgtaaaaag
    36421 ttgtatggag ttctcgatgc agtcggcgcg actatcagta cccccgctga accctggact
    36481 atcgggccgc cgtactgggg catgtcaccc tggcgtgccg ctggttttcc gctaatggtt
    36541 tatttcctct ttctttcccg gcaagaattt attttaatcc ggaaggatta tttattttca
    36601 gatagggact cacattaaac acgtaacatt ttaattaatg catggctgaa tatgtattac
    36661 acaatgtcag atatcgtaaa agaccagtgc aattctatgt aaactctctg actgttcatt
    36721 ttatttgttg tttcagggtc ggaactcacc ctcaataaac aactgaatac gagtagatat
    36781 tcatcgtctg agctatatgg ctttacacaa tagccgacat gtcttttctc agtcaggaat
    36841 tttacttttc ctcactacga agttgacgac ttcgatatgg gatagactct taattcaagc
    36901 aattactctg cacaagcatc aggcaatcca gctccaatat ttgttataaa ataatgcgta
    36961 acattaattt caccaacaaa aaaaaggttt acaccatccc cttgccctgg tatcttacca
    37021 tatttacacc acactttttt tgtatctgga tcattctttg atacgatttt cgccacttgt
    37081 tccctggccc tcactgtcaa tgttttctta aacctacccc catcatcatt tgttagggaa
    37141 aaataagaaa aaaaataatc atcttctaca tttgtgtata ttgatatatc caccgctacg
    37201 tgcggaaaaa aatctgggag ccatgtaaaa actccatcat tttctggtat tgccatatag
    37261 tttatgtaat gtttacttac aagagattca tctttgtata aataaagata agtaattacg
    37321 cataaaacaa caatgattat aatagcaaaa ataaatatta tcatctttga tagattactt
    37381 gagatagcca gcatcttgta aagcctttat cgttttttta tgctctggat taatataatc
    37441 actacatcta tctgagcaat ctgttgttga tggacatgtc aacccatggt catttacagc
    37501 caagtctttt ttattatctt caatcatttc agaatgggat agctttactt ttctgccgta
    37561 cataccgaac agatttaacc cgtaatctct aatctctttt tcataaccca gccctttcgc
    37621 cgatcggcta acaccagcgt catttaattt agacactcga caaaatgcca tacaatggaa
    37681 aaattgatca gtcccaccta aatttaatct cttcatatct tcataagtat cagataatat
    37741 atcccatgct ctttgatggt ttagttgatc ttttcttctt attagcgcta catctgcggg
    37801 acttaaccct aatggatcaa taccattcac cggattcagc gggtacgcat acagactcca
    37861 tccccccctc cagccctatc gggtcctg
//
LOCUS       DAHBHC010000047        37813 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHBHC010000047 DAHBHC010000000
VERSION     DAHBHC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.047"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..334)
                     /gene="tolA"
                     /locus_tag="M2A35_002812"
     CDS             complement(<1..334)
                     /gene="tolA"
                     /locus_tag="M2A35_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCB7199091.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERL"
     gene            complement(399..827)
                     /gene="tolR"
                     /locus_tag="M2A35_002813"
     CDS             complement(399..827)
                     /gene="tolR"
                     /locus_tag="M2A35_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCB7199092.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(831..1523)
                     /gene="tolQ"
                     /locus_tag="M2A35_002814"
     CDS             complement(831..1523)
                     /gene="tolQ"
                     /locus_tag="M2A35_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCB7199093.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1520..1924)
                     /gene="ybgC"
                     /locus_tag="M2A35_002815"
     CDS             complement(1520..1924)
                     /gene="ybgC"
                     /locus_tag="M2A35_002815"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCB7199094.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(2074..2367)
                     /gene="ybgE"
                     /locus_tag="M2A35_002816"
     CDS             complement(2074..2367)
                     /gene="ybgE"
                     /locus_tag="M2A35_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCB7199095.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(2367..2480)
                     /gene="cydX"
                     /locus_tag="M2A35_002817"
     CDS             complement(2367..2480)
                     /gene="cydX"
                     /locus_tag="M2A35_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCB7199096.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(2495..3634)
                     /gene="cydB"
                     /locus_tag="M2A35_002818"
     CDS             complement(2495..3634)
                     /gene="cydB"
                     /locus_tag="M2A35_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCB7199097.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(3650..5218)
                     /gene="cydA"
                     /locus_tag="M2A35_002819"
     CDS             complement(3650..5218)
                     /gene="cydA"
                     /locus_tag="M2A35_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCB7199098.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(6065..8698)
                     /gene="mngB"
                     /locus_tag="M2A35_002820"
     CDS             complement(6065..8698)
                     /gene="mngB"
                     /locus_tag="M2A35_002820"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCB7199099.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(8716..10631)
                     /gene="mngA"
                     /locus_tag="M2A35_002821"
                     /pseudo
     CDS             complement(8716..10631)
                     /gene="mngA"
                     /locus_tag="M2A35_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            10800..11522
                     /gene="mngR"
                     /locus_tag="M2A35_002822"
     CDS             10800..11522
                     /gene="mngR"
                     /locus_tag="M2A35_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCB7199100.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(11626..12495)
                     /gene="sucD"
                     /locus_tag="M2A35_002823"
     CDS             complement(11626..12495)
                     /gene="sucD"
                     /locus_tag="M2A35_002823"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCB7199101.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12495..13661)
                     /gene="sucC"
                     /locus_tag="M2A35_002824"
     CDS             complement(12495..13661)
                     /gene="sucC"
                     /locus_tag="M2A35_002824"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCB7199102.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(13755..14972)
                     /gene="odhB"
                     /locus_tag="M2A35_002825"
     CDS             complement(13755..14972)
                     /gene="odhB"
                     /locus_tag="M2A35_002825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCB7199103.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQIIGRLREGNSAGKETSAKYEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESVP
                     AAADPAAQQALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(14987..17788)
                     /gene="sucA"
                     /locus_tag="M2A35_002826"
     CDS             complement(14987..17788)
                     /gene="sucA"
                     /locus_tag="M2A35_002826"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCB7199104.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQI
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(17989..18705)
                     /gene="sdhB"
                     /locus_tag="M2A35_002827"
     CDS             complement(17989..18705)
                     /gene="sdhB"
                     /locus_tag="M2A35_002827"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCB7199105.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(18721..20487)
                     /gene="sdhA"
                     /locus_tag="M2A35_002828"
     CDS             complement(18721..20487)
                     /gene="sdhA"
                     /locus_tag="M2A35_002828"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCB7199106.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(20487..20834)
                     /gene="sdhD"
                     /locus_tag="M2A35_002829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(20487..20834)
                     /gene="sdhD"
                     /locus_tag="M2A35_002829"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCB7199107.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(20828..21232)
                     /gene="sdhC"
                     /locus_tag="M2A35_002830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(20828..21232)
                     /gene="sdhC"
                     /locus_tag="M2A35_002830"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCB7199108.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            21613..21720
                     /locus_tag="M2A35_002831"
     CDS             21613..21720
                     /locus_tag="M2A35_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199109.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            21926..23209
                     /gene="gltA"
                     /locus_tag="M2A35_002832"
     CDS             21926..23209
                     /gene="gltA"
                     /locus_tag="M2A35_002832"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCB7199110.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            23600..24166
                     /locus_tag="M2A35_002833"
     CDS             23600..24166
                     /locus_tag="M2A35_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7199111.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            24217..26676
                     /locus_tag="M2A35_002834"
     CDS             24217..26676
                     /locus_tag="M2A35_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7199112.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSILLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            26691..27422
                     /locus_tag="M2A35_002835"
     CDS             26691..27422
                     /locus_tag="M2A35_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7199113.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            27419..28480
                     /locus_tag="M2A35_002836"
     CDS             27419..28480
                     /locus_tag="M2A35_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7199114.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            28632..29678
                     /locus_tag="M2A35_002837"
     CDS             28632..29678
                     /locus_tag="M2A35_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCB7199115.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIEHPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(29675..30466)
                     /gene="nei"
                     /locus_tag="M2A35_002838"
     CDS             complement(29675..30466)
                     /gene="nei"
                     /locus_tag="M2A35_002838"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCB7199116.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(30502..31236)
                     /gene="pxpA"
                     /locus_tag="M2A35_002839"
     CDS             complement(30502..31236)
                     /gene="pxpA"
                     /locus_tag="M2A35_002839"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCB7199117.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(31226..32158)
                     /gene="pxpC"
                     /locus_tag="M2A35_002840"
     CDS             complement(31226..32158)
                     /gene="pxpC"
                     /locus_tag="M2A35_002840"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCB7199118.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(32152..32808)
                     /gene="pxpB"
                     /locus_tag="M2A35_002841"
     CDS             complement(32152..32808)
                     /gene="pxpB"
                     /locus_tag="M2A35_002841"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCB7199119.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(32831..33574)
                     /gene="ybgI"
                     /locus_tag="M2A35_002842"
     CDS             complement(32831..33574)
                     /gene="ybgI"
                     /locus_tag="M2A35_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCB7199120.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            33845..35326
                     /gene="dtpD"
                     /locus_tag="M2A35_002843"
     CDS             33845..35326
                     /gene="dtpD"
                     /locus_tag="M2A35_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCB7199121.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(35368..36786)
                     /gene="phrB"
                     /locus_tag="M2A35_002844"
     CDS             complement(35368..36786)
                     /gene="phrB"
                     /locus_tag="M2A35_002844"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCB7199122.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLTAYEEARKGA"
     gene            complement(36783..37292)
                     /locus_tag="M2A35_002845"
     CDS             complement(36783..37292)
                     /locus_tag="M2A35_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCB7199123.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(37443..37813)
                     /locus_tag="M2A35_002846"
                     /pseudo
     CDS             complement(37443..37813)
                     /locus_tag="M2A35_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         9292 a        10147 c         9179 g         9195 t
ORIGIN      
        1 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
       61 gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg
      121 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
      181 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
      241 tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
      301 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
      361 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
      421 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
      481 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
      541 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
      601 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
      661 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
      721 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg tacaatgttg
      781 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
      841 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
      901 ttgtcgtaat tcagttccag tttgtttacg cgctggttga ggcggttata agccattacc
      961 gccggaattg cggcgaacag accaattgca gtcgcaatca acgcttctgc gataccgggc
     1021 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc
     1081 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
     1141 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
     1201 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
     1261 ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctggctc
     1321 tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
     1381 tcgcgcgccg ctgcgttaag aatacgggtc cgctggataa taatggccca agatgcgatt
     1441 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
     1501 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
     1561 gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
     1621 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
     1681 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
     1741 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
     1801 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
     1861 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
     1921 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
     1981 gcgcgattga ggtttgggaa tgagggcaag ttaagggagc gtaaaaattt atgggcctgg
     2041 agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac
     2101 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
     2161 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
     2221 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
     2281 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
     2341 atacaaagtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
     2401 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
     2461 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
     2521 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca
     2581 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
     2641 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
     2701 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
     2761 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcggc agaaccacac
     2821 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
     2881 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
     2941 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
     3001 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
     3061 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
     3121 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
     3181 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
     3241 gcggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt
     3301 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
     3361 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
     3421 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
     3481 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
     3541 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
     3601 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
     3661 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
     3721 cgaggcgtgc aaatttgaac attaagaaca attctgccac caggaacagg gtatacaggc
     3781 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgag ttcgccacag
     3841 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
     3901 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
     3961 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagaa
     4021 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
     4081 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
     4141 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
     4201 ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
     4261 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
     4321 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
     4381 cctcttcctg atcaggaatg ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc
     4441 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
     4501 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
     4561 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
     4621 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
     4681 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
     4741 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
     4801 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
     4861 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
     4921 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
     4981 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
     5041 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
     5101 agaccgtttc cataatggcc agcaggaacg ccataccgag cgtcagtggc acaaaaagga
     5161 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
     5221 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
     5281 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
     5341 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
     5401 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
     5461 ggtgatcaat ttatagctaa attaccgcct ttcagccaat ttgatcgaga acaatttatc
     5521 tctttttgat gcccatttcc aagacttata cattgataaa tatcaattcc tacaaattat
     5581 gttaatcatg tttcaataag gtaactttag tgacatttat gtttaaaatg tgtgagttat
     5641 aaggctgaaa ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca
     5701 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattac
     5761 taataaaggc ataaatccac cccgctgata ttaacattta attaacttat aattatatta
     5821 taattaactc acccagggtt attattttgt taaaaataaa gccacatata cgtggcttga
     5881 gagtgaagat aaacgcaaaa ttacctgatg cgctacgctt gccagtgtct acatcatctc
     5941 tgcaatatat tgaatttgcc tgcttttgta gaccgaataa agcgttcatg ccgtatccgg
     6001 catgaacaaa gcgccctttg tcaacaatct ggccgcgcat atgtggcttc ggtttgattg
     6061 ctattcaggc aagccggtaa ctgaacgtcc gtgactggcc gggtaaaaaa ggccccgata
     6121 gatttgaacc ttgattttcc tcggtggtaa tgtgttcatc catcatcgtt tctgagcaag
     6181 aaatcacctc gcgactgaaa gcaacagtcg catcacaggt tgctgattca gccggattaa
     6241 acagccgtaa aatcacttct tgtcggtctt cagctttctt aagtgcgctt atcaggcatc
     6301 ccactggggg cattttcaac aaactataac tttccggcac gttgaatccg gctttgttga
     6361 gcttcatcgc atcccatggg attttgttgt aacactgtac tggagtcagc catgctcgcg
     6421 cctgctgagc tacaccagcg gcggttggcg taccggtata actcaataaa cttaggcgac
     6481 aagaaagcag accacgtagt tgtgagtccg ggactggcat tttaattccc gaaggccgcc
     6541 caggccttaa aaacagatct tctttgccca gtaagcccac gccacgcagc aacgtaatgg
     6601 caaaggtttt cttctcttca ccgatgactt caaattcacg tagcccttcg ctaaagacag
     6661 ccatgccgtt acgcccttcc tgtaaggctg catagttgag catattccat accggaaccg
     6721 gcgcttcttt ccagccttct tgctgccagt tgttcattgc actgtcgttc acggggcgcg
     6781 ttagcgaacc aaactgggta tctgccagaa cactatcggt gttaaaagat gtagggatca
     6841 ggacgcgaag gcgatgatcg tcagcctggt tatcaaggtt gatatccaca tcaatacgtc
     6901 tgctgttatg actcagagtg acaaccatct ccacccctac tctgcccgtg gattgccgga
     6961 cgcttcgttc caacaaattg agcggcaccg ccatgtcata gcggataaca gccctgctct
     7021 gccaggcctc atgagtaatc tcgcattgcg gtttcgctgt tgcagaagtc attacccact
     7081 cttcttttgc gggtgaatag tcatattcat caccatcatc agagctttct tcaatttgca
     7141 acacccggtc atagcgcaca ccgctatctt tatctaccag ttgtagcgtt ccatcctcat
     7201 tgagcgcaat ttgccagaaa gcattttcca gtatcccttc agcgtcactt tttgccgcaa
     7261 tcacgttgcc aggctgattc gcttcgatat aaagcgtgcg atagcccata gaagggacaa
     7321 tctggttgat ctgtatatca aactccataa agggatcgta attaccgtaa tgaactattt
     7381 gccgatcgat taggcctgga tcgatctcac gggcatggcg aataaaatac ggtacaggct
     7441 gaccgcgatc gtctcgtaga ttaaactggc tggcgcgcag gcgcacagtg gtgttaataa
     7501 cttcttcccg cggccagggc atcaggttaa acagcacgag tttgtcggca tcactctgtg
     7561 gcatgttatc ggcaattttg cgcatgtaga aacgcagcag attatccgcc atgtcttcag
     7621 ccagttcgaa gcgggcgaca atttcgcgat gaactttgtc actgcaacaa caaccgatac
     7681 tgtcgtgggc atgatttttt aagatctctt tccacatttt ctccagcaag ccgtggtggt
     7741 attcaaaacc caacgtccag gccagcgttg ccagcggttc cagcagatta acaatcttat
     7801 tttcaatacg tgcgtgggca attttgatat ccatgcgcgt ggaaccgatg gtgcgatgta
     7861 cgcgcatata tttgccatcg ataaattcac ctttcagggt ggccagactt tcacgctgcg
     7921 cttcgatctt ctcaaatacc tcttcaaagc ggctcatcac aaacttacgt tgagggtaga
     7981 tctcacgtag cttatccatc acttcgaaga tattttgctg caatggcatc tggtcatgcc
     8041 cattcggcag caaaatctct ttggttacag acgctttttc cagcacgtca aaataactgt
     8101 cgaggcgttt acgtaatccg ttttcgtcgg caggtaagta cttaccgatg gcgtagccca
     8161 gcggcagcac ctgcgccgtc acttcgctac cgtcactgct ttgccacaaa aactcggttt
     8221 tatcagtacc gtggcgctcc gaacatccgc gccagaacat ggtgcgggta atgccaaatc
     8281 cattgtagat atgcggcagt tgcccggaca tgccaaagga atctggtaaa taacctattt
     8341 tcatcggctc gccaaacgcg aggcagtcac gcattccgta catcagatta cggacgatgg
     8401 attccgcaga aacaatcgtg gtatcggtct gggtatacca ggggccgata atcaacttgc
     8461 cggcttctac ctgtttcttc acgcggtctt tgttttccgg tttcaccgcg aaataatctt
     8521 cgaggatcgc cgtttgcccg tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc
     8581 ggcacaggat ctcttccata ttattgacca gcagaatgcg tgactcttcg gtggtgaaat
     8641 accactcccg atcccaatgc atatgcgggg tgatgtgaac gcgagatacc gctttcatcg
     8701 tcgtttcctt tttgtttatg gcattacgcc atcagtcaga tagttgccat gcttaaccgc
     8761 gtgacgccgc cagatcagga gaattgcagt cgagattgca gcccccacca atgccgcacc
     8821 aaaccagcca attgccgcca taacaccgcc cgcgccatta tcatgaagta aaaagagcga
     8881 gaaaatgccg gcaccgggtg tcgaaaggcc gatattcatc gcaccgacaa tagcgcccgt
     8941 taccatagag cccagcacaa acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg
     9001 gatcgcccct tcggtaatac ctgccagccc taacagccag gtggatttcc cggtctcaat
     9061 ttcaaactct ttaaacaggc gcggtgcgag catcgtggaa gcggttacgg taaatgccga
     9121 aaccattttg acggaggcga aaatggcata cgggccgtaa acgccgttcg ccattgcgcc
     9181 caggcagaat gcataagcgg ctttgttcac tggccctcca aggtcaaagg aacacataaa
     9241 accgagaatg gcacccagca acagcgcgtt acttcctgac agaccgttca gccaggcggt
     9301 aagcgagtta ttgatccagg cgacaggttc tcccaccaca aacagcatca gactgcccgc
     9361 tcccaacgta ccgagcaccg ggtagagata aaaagtcagg aatccattga atttactgct
     9421 aagacgcaag tgatttttca cccagcgcat caagtaaccg gctatcaatc cgccaacgac
     9481 cgcgccgaga aacccggagc cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc
     9541 taacgccggt ttatctgcca gagaataggc ggtataggcc gcgagcaccg gtaccatcaa
     9601 aattccgagc agcccgccgc ccagcttgcg gtacatccac agccaggagt tttcttcatt
     9661 aaacagatct tgtagcccga agatttgcga cagtaatacc gcgaccgcca gcaccgtgcc
     9721 ccccgcgaca atcaacggta cggcaaaaga gattccgctc aacagtgcct gtttcagctc
     9781 cgttttgaca cttttcaccg gttgcgtatc ttgctgtacg gtacgcgtct catcgctacg
     9841 cttgagggta agcgcttgtt ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg
     9901 cactgaaagc gcgggatgcc attaaaacgc tcactctcct tgatggcgac ttcagccgca
     9961 aaaatacagg cggttgcact attgagttga tccgccgtta aacgcccttc aatgccgtta
    10021 gcgccttgtt tttcaacgta aacatttacg ccgagtttgc gtccggcttt ttccagatat
    10081 tccgcagcca tataggtgtg agcaataccc gccggacagg ccgttacgca gacgatagtt
    10141 ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct tgtcatccag cgccgagagc
    10201 aactcatcag gcgtcgtcgc cgactgtata cgcgcccgaa tctcatcatc cgccaggcgc
    10261 gtggtcagcg ctgtcagcag ttgcatatgc gtagtacccg cttcattggg gggaatcgcc
    10321 agcagcacca ctaaatcaac cgcttccggg ccatcaacgc cttcccactg aagcggctcg
    10381 ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag cagttttgcc atgcggcaca
    10441 gccaaccctt cacctaaggc ggtcgggcca aggctttcac ggcgatacac ttcttccaga
    10501 aattgctcag tactggaaat tttccccaga gcagcaagac gttgagtcaa cgcgtggatg
    10561 gcctcttcac ggctggtaaa gcgcgcattc agacacaacg catcgcggtg ggtcagagtc
    10621 gtcagattca tcatgatcct cacttgatcg tttttatagt cgcgccagtg tgcccgataa
    10681 aacaatacca ttatgtgatc aatttcactt gattgaaatt atttgtattg tatttgtatt
    10741 atttacagga tgaatttatc gggcataatg gctaaattcg tattaatgag atactaaaaa
    10801 tgggacacaa gcccttatac cggcagattg ccgatcgcat tcgtgaacaa attgcgcgtg
    10861 gcgagcttaa acctggcgat gcgttaccta ccgaatcggc attacaaaca gagtttggcg
    10921 tcagccgggt tacggtgcgt caggctttgc gccagttagt cgagcaacag atcctcgaaa
    10981 gcattcaggg cagcgggact tacgtcaaag aagagcgggt caattacgat atttttcagt
    11041 taaccagttt tgatgaaaaa ctgtcggacc gtcacgtcga tacacacagt gaagtcctga
    11101 tatttgaagt gattccggct gacgattttc ttcagcaaca gctacaaatc accgcgcagg
    11161 atcgtgtgtg gcatgtgaag cgcgtgcgct atcgcaagca aaagccaatg gcgctggaag
    11221 aaacctggat gccgcttgcg ttgttcccgg atctcacctg gcaggtcatg gaaaattcaa
    11281 aatatcactt tattgaagaa gtgaagaaaa tggttatcga tcgtagcgaa caggaaatca
    11341 ttcctctgat gccaaccgaa gagatgagcc gtctgcttaa tatcagccag acaaagccga
    11401 ttcttgaaaa agtatcgcgt ggatatttag ttgatggtcg ggtgtttgaa tatagccgca
    11461 acgcttttaa taccgatgac tataaattta ccctgatagc gcaacgaaaa tcatcgcgat
    11521 aagcacaaaa aaggccatca tacgatggcc aaccatgtcg aaaccgacag cccttcgcca
    11581 ttcctgtaga gggaagcggc gagggctatt tcttattaca gatatttatt tcagaacagt
    11641 tttcagtgct tcaccgatat ccgccaggct gcgaacggtt ttcacgcctg cggcttccag
    11701 agcagcgaat ttctcatccg cagtcccttt cccaccggca atgatggcac ccgcgtggcc
    11761 catacgtttg cctttcggcg cagtcacacc agcgatgtaa cccacaactg gcttggtaac
    11821 atgctctttg atgtacgctg ccgcttcttc ttcagcgcta ccgccgatct caccgatcat
    11881 tacgatcgct tcggtctgcg gatctttttc gaacatttcg agaatgtcga taaagttaga
    11941 gccagggatc gggtcaccgc cgataccaac acaggtcgac tgaccgaaac cgtaatccgt
    12001 ggtctgttta accgcttcat aggtcagtgt accggaacgg gaaacgatac ccactttacc
    12061 cggtttgtga atgtgacccg gctggatacc gattttgcat tcacccggag tgataacgcc
    12121 cgggcagttc gggccgatca tacgaacgcc ggcttcgtcc agtttcactt tcacagtcag
    12181 catatccagc gtcgggatgc cttcagtgat ggtgataatc agtttgatgc ctgcatcaat
    12241 agcttccaga atggagtctt tgcagaacgg tgctggtacg tagataacag aagcggtagc
    12301 gccagtagca gcaacagctt cacgcacggt gttgaacacc ggcaggccga ggtgggtagt
    12361 accgccttta cctggggtta cgccgccaac cattttagtg ccgtatgcaa tggcctgttc
    12421 tgagtggaaa gtcccctggc taccagtaaa gccctggcag ataaccttgg tgtttttatc
    12481 aattaaaatg gacattattt cccctccact gcggcaacaa cctgctgagc tgcatccgtc
    12541 agaccttttg ctgcaataat attcaggccg ctgtcagcca gtttcttcgc gccgagttcg
    12601 gcgttgttac cttccagacg taccacgacc ggtacgttaa cacccacttc tgctaccgcg
    12661 ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac cgccgaagat gttaaccaga
    12721 acggctttca ctttgtcgtc agagaggatg attttgaacg cttcggttac acgttctttg
    12781 gttgcgccgc cgccaacgtc aaggaagtta gccggttcgc cgccgtgcag tttaacgatg
    12841 tccatcgtac ccatcgccag acctgcgccg ttaaccatac aaccgatgtt accgtccagc
    12901 gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac gcggatcttc ctgcgactgg
    12961 tcacgcattt cgcgcagatc aggctggcgg aacagtgcgt tgccgtcagc gcccagtttg
    13021 ccgtcgaggc aaatcagatc gccctgtttg gtgatgacca gcgggttgat ttcgatcaac
    13081 gccaggtcgc gctccaggaa aatggtcgcc aggcccatga agattttggt gaactgctga
    13141 accagtttac cttccagacc cagtttgaac gccagctcgc gtccctgata cggcatcggg
    13201 cctgtcagcg gatcaagcgc aactttatgg atcaggtgcg gagtttcttc cgccactttt
    13261 tcgatttcca cgccgccttc ggtggaggcc ataaagacca cgcgacggga actacggtct
    13321 acaacggcac cgagatacag ctctttagcg atatcggtcg ctgcttcaac cagaatctgg
    13381 ttaaccggtt ggccattggc atctgtttga tacgttacca gacgcttgcc gagccagttt
    13441 tctgcaaaag cacggatgtc ttctttgctg tttacaactt tcacaccgcc cgctttaccg
    13501 cggccaccag cgtgaacctg acatttcact acccacgggc cggcaccgat ttttgaagcg
    13561 gcttcttctg cttcgcgcgg agtagtacag gcataaccca ccggtgctgg taagccatag
    13621 cgggcaaaaa gttgttttgc ctgatattca tgtaagttca tgtgttctgt ccatccttca
    13681 gtaatcgtta tcttttaaac cgtagaccga caccctatcc acgtcggtct acagtgcagg
    13741 tgaagcttaa actactacac gtccagcagc agacgcgtcg gatcttccag caactctttg
    13801 atcgttacca ggaagcccac ggattcgcga ccatcgatca gacggtgatc gtaggacagc
    13861 gccaggtaca tcatcggcag gatctcaacc tgaccattca ccgccatcgg acgatctttg
    13921 atagcgtgca tacccagaat tgcgctctgc ggcgggttga tgatcggcgt agacatcagg
    13981 gaaccgaaca caccaccgtt ggtgatggtg aagttaccac cggtcagatc ttcaacggtc
    14041 agcttgccgt cacggccttt gactgccagc tctttgattt tcttctcgat gtctgccatg
    14101 ccgagggtat cgacatcacg cagtaccggt gtcaccaggc cgcgcggcgt agaaaccgcc
    14161 atgctgacgt cgaaatagtt gtggtaaacc acgtcatcgc cgtcgataga cgcgttcact
    14221 tccgggtaac gtttcagggc ttcaaccacc gctttcacgt agaaggacat aaagcccaga
    14281 cggatgccgt ggcgtttttc aaacgcttca ccgtactgct tacgcagatc cataatcggc
    14341 ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg agtttttcgc ttccagcaga
    14401 cgctctgcca cacgcttacg caggcgagtc atcgggacac gtttttcact acgtgcagcc
    14461 agagcctgtt gcgccgccgg atcagccgct gccggaacag actctttcgc cggggctttc
    14521 gccagatgtt tttccacatc ttcacgagtc agacgaccac ccacaccggt gcctttaatg
    14581 gcgctggcgt cgagattgtg ttcagccagc aggcgacgga tcgccgggct taacgcatcg
    14641 ttgttttgct cttccagaga cgcctgctgg cgttgcgccg gagtggacgc tttctcttca
    14701 tatttggcgc tggtttcttt accggcgctg ttgccttcac gcaggcgacc aatgatctga
    14761 cgagacgtta ccgttgtacc ttcatcttcc agaaccgcat ccagaatgcc gtctgctgat
    14821 gccggtactt ccagtaccac tttgtcagtt tcgatttcta ccagcacttc atcacgtacg
    14881 actgcgtcgc cgggtttttt atgccaggtt gcgacggtgg catcggctac ggattcaggc
    14941 aggtcaggga ccagaatatc tacgctactc attgtgtatc ctttatttat tcgacgttca
    15001 gcgcgtcatt aaccagatct tgttgctgtt tctggtgaac ggacatatac cctaccgccg
    15061 gagaggcgga ggccgggcgg cctgcataac gcagagaagc cccaaacgga atcacttcac
    15121 ggaaatgatg ctggctgcag taccatgcgc cctggttgag cggctcttcc tggcaccaga
    15181 caaaatcctt gacgtgagca aactgctgca acacttcctg catcgcttta tgcgggaacg
    15241 ggtagagttg ctcgatacgc acaatggcga catcgtgttg attgttctta cgacgctgtt
    15301 ccagcaggtc gtaataaacc ttaccagaac acattactac gcgcttcacg cccttcggat
    15361 caagctcgtc gatttcaccg atggctggca ggaaggtgcc gttcgccagt tcttcgaggc
    15421 tggaaaccgc cagcggatga cgcagcaggg atttcggcga catcacgacc agcggacgac
    15481 gcatcccgcg cagcgcctga cgacgcagca tgtggtaaac ctgtgccggg gtagacggta
    15541 cgcaaacctg catgttttgc tcagcacaaa gttgcagata acgttccaga cgcgcggagg
    15601 agtgctccgg cccctgccct tcgtaaccgt gcggcagcaa catcaccaga ccacacatcc
    15661 ggccccattt ctgctcgcca gaggagatga actggtcgat aaccacctgc gcaccgttgg
    15721 cgaagtcacc gaactgcgct tcccagatgg tcagggtgcg tggttctgcg gtggcataac
    15781 cgtattcaaa cgccagcact gcttcttcag acagtacgga gtcccaaaca cggaacgcgc
    15841 cctggccgtt atggatgtgt tgcagcggcg tgtaagtgga accgttagac tggttgtgga
    15901 tcaccgcgtg gcggtggaag aaggtaccgc gaccggagtc ttcacccgac aggcgaaccg
    15961 gaatgccttc gtcaaccagc gtggcgtaag cgaggttttc cgcgccgccc cagtcgaaca
    16021 gtttctcacc ggcagccatc gcctggcgat cgccataaat cttggcaacg cgagactgca
    16081 tttcaaccgc ttccggcacc gtgctgatgc gtttagccag ttcctgcagg cgcttcatct
    16141 caactttgtt cgggtactct tcgtcccatt cgtggttgag gtacggcgac caggtgaaag
    16201 agtgcatgtt catcggacgc cactctgcta ccacgcaatc gccagcatcc agcgcatcgc
    16261 ggtacaggtt aaccatctcg gtggcatctt ccagcgtcgc cactttttcc tgctccagct
    16321 tgtcagcgta gattttgcgc ggtgtcggat gttttttgat tttctgatac atcagcggct
    16381 gggttgcgct cggctcgtcg gcttcgttgt ggccgtgacg gcggtagcat accaggtcga
    16441 tgaagacatc acgtttaaag gtgttacgga aatcgagcgc cagacgggtc acaaaggcaa
    16501 cggcttccgg atcgtccgcg ttaacgtgga aaatcggtgc ctgaaccatc ttgccgatat
    16561 cagtacagta cggcgtagaa cgggcatcca gcggattaga ggtggtgaaa ccaatctggt
    16621 tgttgataac gatacgtacc gtaccgccaa cttcataacc acgcgctttc gacatgttca
    16681 gggtttcctg aaccacgccc tgtccggtca ctgcggcgtc accgtggatg gtaattggca
    16741 gcactttgtt gctgctcggc tcatcaagtc tgtccagacg ggcacgaaca gagccgataa
    16801 cgaccgggct tacaatctca aggtgagacg ggttaaacgc cagcgccagg tgcaccaggc
    16861 cgccatcggt ctggaagtca gacgagaagc ccatgtggta tttcacgtca cccgtgccga
    16921 ggtgttcttt atgtttaccg gcaaactcgt cgaacaagtc ttgcggtttt ttacccagca
    16981 cgttcaccaa cacgttcaga cgaccacggt gcgccatccc gagaaccact tcccgggtgc
    17041 cgctgttgcc agcgtggcgg atcatctctt taagcatcgg gattaacgcg tcaccgcctt
    17101 ccagcgagaa gcgttttgcg ccagggaatt ttgcgccgag gtaacgttca aggccttcag
    17161 cggcggtcag ttcgcttaag aagcgttttt tctcttcgct attgaaagtc gcgcgaccag
    17221 actcgatacg ctgttggatc cagcgttttt cttcggtgct ggtaatgtgc atatactcgg
    17281 caccaatcgg gccgcagtag gtttgcttga gggcttccag cagctcgccg agtttcatgg
    17341 tttctttgcc gctggcaaat gaaccgacgt tgaaggtctc ctggaagtct gcttcggtca
    17401 gatcgtggaa agacggatcc agatcggcca ctttatcttg ctgccacagt cccagcggat
    17461 cgagattcgc atgctggtga ccacggaagc ggtatgcgtt aatgagctgc aggactttaa
    17521 cctgctttac attggtgtca gggtcggaga tcgttgaaga gtaacgtgaa gcgtctttcg
    17581 ccaggcggcg gaaatattca cgcgtttgag agtggaattg atccggtttg actcccgtac
    17641 caggtaactg ctggaacgtc gaacgccagt tagcgtcaac cgagtcagga tcggttaaga
    17701 agtcttcata gagctgttct atccagctct ggtttgcgcc agagaggtaa gaagagtcca
    17761 accaggcttt caaagcgctg ttctgcatcg tgatccctta agcatctttt ttatgcttac
    17821 ttcgccgtgg atactaccac gcacagttat gtgcgtgccg gggttcactt gcgagctctt
    17881 gtctacgtag tggctcgcga aggaaccttt aaaaactgtc ttagtgtcag gggcagtttt
    17941 taaagatttc ctgaatagcc gggggatttc cccggcatca acatttaatt acgcattacg
    18001 ttgcaacaac atcgacttga tatggccgat ggcgcgcgtc gggttcagcc ccttcggaca
    18061 tacactgacg cagttcatga tgctgtgaca gcggaataca ctgaatgcat cgctcaaacc
    18121 gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc aggaaacgat atgccgctaa
    18181 caagcctgcc gggccgataa acttatcggg attccaccag aaagacggac aagaggttga
    18241 acaacatgcg cagagaatac attcatacag cccgtcgagt ttttcgcgct gctctggcat
    18301 ctgtaaatgc tcgcgagctg gcggattttg tccattattc aacaggtaag gcttaatttt
    18361 ctcatattgc gcatagaatt gtcccatgtc taccaccaaa tcgcggatca ccggtaaacc
    18421 tggcagcggg cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa tcggggtaat
    18481 acaggccaga ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca caccttcacg
    18541 gcaggagcgg cggaacgaca ggctgggatc tttctctttc agctggataa gcgcatccag
    18601 caacatcatg tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac gcggagcatc
    18661 atcaacatcc gggttatagc gataaattga aaactcgagt ctcattttcc tgtctccgca
    18721 ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca tgttgacgct
    18781 tcggcgcgtc atggattccg actctggcag atacagggag tggcacagcc agttttcatc
    18841 atcacgatcc gggaagtcga agcggctatg cgcgccacgg ctttcggtac ggaagttggc
    18901 agaaacagcc gttgcatacg ccgtttccat caggttatcc agttccaggc actcaacgcg
    18961 ctgggtgttg aactcgctgg aagtgtcatc cagacgggca tttttcagac gctcgcggat
    19021 aactttcaac tgctcaagcc ctttcgccat cgcatcacct tcacggaaga ccgagaagtt
    19081 atgctgcata cattcttgca gcgctttacg aatcgccacc ggatcttcac cgttacgatt
    19141 attgttccag cggttcagac gatccagaga tgcttcaaca tcagactcgc tggcatcgcg
    19201 caatgcgccc tgctcggcga tagactcttg cagatgcaga cccgctgcgc gaccaaagac
    19261 caccaggtcc agcagcgagt tgccgcccag acggttagcg ccgtgtaccg atacacaagc
    19321 gatttcacca acggcaaaca gccccggaac aaccacatct tcgcctttct cgttcaccgt
    19381 cagcgcctga ccggtcactt tggtcggaat accacccatc atgtagtgac aggttgggat
    19441 aaccggaatc ggctctttca ccggatcaac gtgagcgaag gtacgggaga gttccagaat
    19501 acccggcaga cgggattcga gaacttcttt acccaggtgg tcgagtttca gtttcgcatg
    19561 tggcccccac ggaccatcac agccgcgacc ttcacggatt tcaatcatga tggaacgcgc
    19621 caccacgtca cggcccgcca ggtctttggc gttcggcgca taacgctcca taaagcgttc
    19681 gccatgtttg ttcagcagat aaccgccttc accacggcaa ccttcggtga ccagtacgcc
    19741 cgcaccggca ataccggtcg ggtggaactg ccacatttcc atatcctgca ccggtacgcc
    19801 tgcgcggata gccatgccga cgccgtcgcc agtgttaatg tgggcgttag tggtggactg
    19861 ataaatacgc cctgccccgc cagtcgccag cacggtagcg cgggctttga aataaaccac
    19921 ttcaccagtt tcgatgcaca gtgcagtaca acccaccacc gcgccatcct ggtttttcac
    19981 cagatccagc gcataccact cggagaaaat agtggtgtgg tttttcaggt tctgctgata
    20041 aagggtatgc aacagtgcgt gaccggtacg gtcagctgct gccgcagtgc gtgccgcctg
    20101 ctcgccgccg aagtttttcg actgaccgcc aaacggacgt tgatagatac gaccatcatc
    20161 aagacgcgag aacggcaggc ccatgtgttc gagttccaga atcgcttccg gcccggtttt
    20221 acacatatat tcaatcgcgt cctggtcacc gatatagtcc gaacctttta cggtgtcgta
    20281 catatgccat tcccagttat cttcatgggt attacccagc gcaacggtaa tgccgccttg
    20341 cgcagaaacg gtatgggaac gggtcgggaa gactttagag agcagcgcac aggtttgacc
    20401 gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa tcacaactgc
    20461 atcaaattct ctgactggca atttcatcac acaccccaca ccacaacgaa tccataaatc
    20521 acgtaaacca ccagagcaac gacaatcacc agttgcagca tcaggcgcaa ggccagcggt
    20581 ttaacgtagt cggtcaacac ctgccacatg ccgatccagg catggatcaa gatagaaaac
    20641 agcgccagca gggtgaacac tttggtgaac gcagaggcga agaaaccgat ccagacttca
    20701 tatgtcagct cgccactggt agcgaaaaaa ccgaccatat aaatgatgta gagcgtcagg
    20761 acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc taatgcggag
    20821 gcgttgctta ccatacgagg actcctgcga gaagtgaaag cacgacagta ataacaaagg
    20881 agattttggc ggagcgttta cccgcttcga atgtttcttc cagatagcca aaatccatca
    20941 tcatgtggcg aatacctacg acgacgtgat acgccagagc ggtaaggatg ccccacatga
    21001 taaatttgac gaagaagctg cccataatcg cggaagcttg ctcgaaacct tcaggggaag
    21061 agaggctggt acccagaagc cacagcagga tacccactgc aataaaggtg atcacaccgg
    21121 aaacgcgatg gagaatggac gctatcgccg tgatggggaa ccggatggtc tgtaggtcca
    21181 gattaacagg tctttgtttt ttcacatttc ttatcatgaa taacgcccac atgctgttct
    21241 tattattccc tggggactac gggcacagag gttaactttc tgttacctgg agacgtcggg
    21301 atttccttcc tccggtctgc ttgcgggtca gacagcgtcc tttctataac tgcgcgtcat
    21361 gcaaaacact gcttccagat gcgaaaacga cacgttacaa cgctgggtgg ctcgggattg
    21421 cagggtgttc cggagacctg gcggcagtat aggccgttca caaaatcatt acaattaacc
    21481 tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata acaacattta
    21541 tttaattttt aatcatctaa tttgacaatc attcaacaaa gttgttacaa acattaccag
    21601 gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat taacttatgt
    21661 aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtag acaagtttaa
    21721 taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga tcctttacct
    21781 gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg cgagccaaat
    21841 aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg
    21901 taataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca ccctcaacgg
    21961 ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg ttattgatat
    22021 ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt caaccgcatc
    22081 ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc accgcggttt
    22141 cccgatcgat cagctggcga ccgattctaa ctacctggaa gtttgttaca tcctgctgaa
    22201 tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga cccgtcatac
    22261 catgatccac gagcagatta cccgtctgtt ccacgctttc cgtcgcgact cacatccaat
    22321 ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact cgctggatgt
    22381 taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa tgccgaccat
    22441 ggccgcgatg tgttacaagt attccattgg tcagccattt gtttatccgc gcaacgatct
    22501 ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt
    22561 taatccgatt ctggaacgtg ctatggaccg tattctgatc ctgcacgctg accatgaaca
    22621 gaacgcctct acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc cgtttgcctg
    22681 tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta acgaagcggc
    22741 gctgaaaatg ctggaagaaa ttagctccgt taaacacatt ccggaatttg ttcgtcgtgc
    22801 gaaagataaa aatgattctt tccgcctgat gggcttcggt caccgcgtgt acaaaaatta
    22861 cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag agctgggcac
    22921 caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga acgacccgta
    22981 ctttatcgag aagaaactgt acccgaacgt cgatttctac tctggtatca tcctgaaagc
    23041 gatgggtatt ccgtcttcca tgttcaccgt cattttcgca atggcacgta ccgttggctg
    23101 gatcgcccac tggagcgaaa tgcacagtga cggtatgaag attgcccgtc cgcgtcagct
    23161 gtatacagga tatgaaaaac gcgactttaa aagcgatatc aagcgttaat ggttgattgc
    23221 taagttgtaa atattttaac ccgccgttca aatggcgggt taatttttat atgcctaaac
    23281 acaaaaaatt gtaaaaataa aatccactat cggacattca tatattctaa aaaaaaatat
    23341 atcggtataa attataaaca aatcgatgat ttttaatcat ctatcttcct ctcagttaca
    23401 tgaataaacc accaaatata gtgcacccct ataatttcca atactttatt catcaaacat
    23461 ttaattcata ccatcaatta attactttta catttaataa aattaataat atttttaaga
    23521 aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac ttttttcttt
    23581 tctgttaaag gactccgtta tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc
    23641 tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa
    23701 aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga
    23761 actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg ccgttgctgt
    23821 cgacctgcgc ttagtcaact gtgatctgga aaactccagc aacggttccg gtggcaagat
    23881 ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag atccgattct
    23941 caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga ataaagatca
    24001 aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga cctccagcga
    24061 acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac ctgtaacgcc
    24121 aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat tactttggcg
    24181 gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acaccgtgaa tatttatcga
    24241 ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt cgaattcaac
    24301 cttaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt aaaggaaaaa
    24361 ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa ccaaatcagt
    24421 aacgggcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg catcaatgat
    24481 ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc attgataaat
    24541 cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca agccaatcag
    24601 caactaaata tcaccattcc gcaagcctgg ctggcgtggc actcagaaaa ctggacccca
    24661 ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt gtttgccagc
    24721 agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg aacagccgga
    24781 atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcagac tgatagcgat
    24841 gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc gttaccgcaa
    24901 ttaggttcaa aattaaccct cggcgaaacg gattttagtt ccaatatttt cgacggtttt
    24961 tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga actacgcggc
    25021 tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat cagtcaatca
    25081 ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga cgaccttaat
    25141 cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat
    25201 ttccaggttt cggcagcatc gacgcccttc ctgactcgtc agggacaggt tcgctataaa
    25261 ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga aacctttttt
    25321 agcaatgaag tttcctgggg gatgctgtca aacacctcgc tgtacggcgg cctgctgctt
    25381 tctggtgatg actaccattc tgccgcaatg ggtattgggc aaaatatgct gtggcttggt
    25441 gcgctgtcgt ttgatgtcac gtgggccagt agccattttg atactcagca ggacgagcgg
    25501 ggcttaagct accgttttaa ttacagcaaa caagtggatg ccactaacag cacgatttcc
    25561 ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta tctggatcac
    25621 aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc tgtgggccaa
    25681 ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac ctggtggaat
    25741 gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat tggtgactgg
    25801 agagatatct ctatttcgac gtcattcaat acaacccatt acgaagataa agatcgcgac
    25861 aaccagattt acctgtcgat tttgctcccc ttcggtaacg gtggtcgggt tggctatgac
    25921 atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac gctggatgaa
    25981 cgtaatagct ggggcatgtc tgccggactg caatccgacc gtcctgacaa tggagcccag
    26041 gtgagcggta actatcagca cctgagttca gcgggtgagt gggatatttc tggtacctat
    26101 gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac cgcaacccaa
    26161 tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat ggtcagcacc
    26221 gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca ttttggcatt
    26281 gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa catgaatgac
    26341 ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg
    26401 ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac
    26461 gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag cggcattagc
    26521 gtggggatgg ttggcgagga aggacatgct tggttaagcg gagtcgctga aaatcaaaag
    26581 tttaccgtgg tctggggtga tagccaacat tgctcgctcc atcttcctga acatatggaa
    26641 gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa atgacattta
    26701 tgaaaggact accccttttg ttgttggttg ccagtctgtg cagccacgct gcactacaac
    26761 ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg cgggtggata
    26821 atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa aaaggggaga
    26881 aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg aaagcaacaa
    26941 cgcaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat cgcgaaacgc
    27001 tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc gaccatgccg
    27061 ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct gcattacgca
    27121 agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccaacagggc aaccaactga
    27181 cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga aatgaaaaag
    27241 gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc aacacaaaca
    27301 ccggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac ggcgcgttac
    27361 gcatgacgac gctcaactgc agcggacaat gccatttaca agccgtggag gcgaagaaat
    27421 gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgccgt tgaagtctgc
    27481 gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat cagaagcgat
    27541 tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatttggg aatctgacga
    27601 tattaaaatt cccgtctatt gtgacaacaa taccaacggc aattttgaaa gtgagcatgt
    27661 ttatgcctgg gtaaacccct acccgggggt gcaggatcgc tattatcaac tcggcgtaac
    27721 ttacaatggc gttgattatg acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg
    27781 tattgacagt aagaacattg acatttatac ccctgagcag attatcgcga tgggatggca
    27841 gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcacgtacct ttctcgctcg
    27901 catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa gtacgcttag
    27961 cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagatc ctactgccca
    28021 aaacctgaaa tatcatatta ccggtctgga aaacattcgc gtgctggatt gcagcgtcaa
    28081 tttttccatt tcaccggaaa cacaggtgat tgattttggt aaatttaatt tgctggatat
    28141 tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac aaaatgacca
    28201 atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt tggttgaaga
    28261 agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg aaaacgcctc
    28321 gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca tgttggtcta
    28381 cgaaaaaacc tatacagctg aactttcgtc cattgcaggt acccccatcg aggctgggcc
    28441 cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga tgccctttgc
    28501 agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct tgattgctgt
    28561 tattttttcc tggcccggct ttctttatgc tgtatcgata caccaaagag aaaataatga
    28621 gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca cttctccttt
    28681 ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg atgatcgccg
    28741 ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct tttctcgccg
    28801 cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt ctcaccacgc
    28861 tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt tccagcgcca
    28921 tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt gcctggggtt
    28981 cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc gcagatattc
    29041 gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg
    29101 tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc gtctggttcc
    29161 cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg
    29221 gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg ggcgcagtgc
    29281 tccagtctgg tcagctcatc accatcgaac acccggaatg gttgctggcg atggcgtata
    29341 tggcaattgg ttggcggatt ggtcttggtt tcgataaaca aatcttactg cgggcattgc
    29401 gcccactgcc acaaatcctg ctgtcgattt ttgctctgct ggctatttgt gcgggtatgg
    29461 cgtgggggct gacccggttt atgcacattg attttatgac tgcctacctc gccaccagtc
    29521 ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat atggcgctta
    29581 ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacggggcca gccattgcgc
    29641 ggtttatttc aacctatgcg ccgaagcggt cggcctagtg ctggcagcct ggacaccagt
    29701 aaaacgggcg cgaggacaac gtggttttct caatgatgcc gccacagcgt tcgcacagtt
    29761 cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta ttctcatcca
    29821 cctgcccccg cgtagcgtaa gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat
    29881 ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca acctgccaga
    29941 ggatctccac ccgcaaataa ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc
    30001 ctgcaaactg acggttacga aagcgcggtg acaataaccg ttcctttacc acctccggcg
    30061 tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt
    30121 gttccggcct caacatctca atatccgagg cgctataaag cagaatggtt ttgtcagccg
    30181 tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc
    30241 gccagacgcc gtaaagctga ttatggctgt agagcgttaa gtcgttggaa aaatgagtta
    30301 acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt tgtgattgat
    30361 aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct ttgatcgccg
    30421 cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg atatcctttt
    30481 ttcattcttt attaatcggg gttatgctgc gacaacaatc tccttttcgg caaatgtaga
    30541 gcgtagtcgg cgggcgaacg ccagtgcgtg ctcgccgtcg ccatgcaggc agactgtttg
    30601 cgccgtgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt gcaccatctc
    30661 cagcgtttgc gccaacgcct gttcttcgtt ttcaatcaac gcgcctcgct gacttcgcgg
    30721 caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc gcgttgtcag
    30781 gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga gaatcaatgc
    30841 cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc
    30901 ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt gcgcacgggc
    30961 aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt ctggcggcag
    31021 ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc
    31081 gacaccattt tttattgctt cacgcacgca agcctgcatg atttgcgcat cgcctgcatg
    31141 aaatccacag gcaatattgg cagaggaaac cagcgttaat agctctgcgt cgctggcgca
    31201 gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac gctaattgtt
    31261 cgaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa
    31321 aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta catatcagcc tcaatgatac
    31381 aggcaatacg cgggtaacca ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc
    31441 cgttatgcgg cacctgcacc acgcccggta acaaaccgtg agataacagt tcgcgatcgg
    31501 tgatgcgttt taaaatttgc ccctgtaagc gatagcccat tcggttactt tgcgagctaa
    31561 gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca tgatattccg
    31621 gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc tgcgcttcca
    31681 taaaatcacg cttcgatttg ccaatcggga gtcggtcacc atccttcagt aaacggcctt
    31741 ccagcccgcc aatccccact ttgagatcgg tgctgcatga ccccattacc ggcggaacat
    31801 caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta
    31861 aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcatta tcatccagcc
    31921 gcgcttcgca accggcaccc gtcagagcaa accacccatc agtttcgaac tcaaccgtta
    31981 actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc agcaggttag
    32041 caatgcgtaa cgcaggcata tccagtgcgc cgcagtggct gatacccgac tggcgaaaac
    32101 cgtgacgacc gccatcttgc acagtggtat acatgcccgc acgaataatc ttcagcatac
    32161 tccctccttt tgcggtacaa agcgcacgct gtctcccgga cgtaataaga tgggttcgtc
    32221 acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccacc cacccggcgt
    32281 tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg ccggaacgag
    32341 caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg
    32401 gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt gcaattcaac
    32461 aacctgtttt tcgctcaacc cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc
    32521 accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg cctcgctctc
    32581 ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag gattacgcaa
    32641 aatcaccgtg atattgttca tgccgggaat ggcttcaacc acattcggca tatccaccag
    32701 acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg gttccagttc
    32761 cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt tcgatccctc
    32821 tgattattcg ttatgcagga ttaggtatat caataaaggt cacatcaaga tcggtatttt
    32881 cattcagcca ctcactcaat gcgcgaatac caccgcgttc agtggcatgg tgacctgcag
    32941 cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa acttcgccag
    33001 taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg
    33061 tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat aacggcttac
    33121 gtcccagacg cgcttcaatc caggaggcta gttccagtcc cggcacaggc atggtcagct
    33181 ctccccacgg caccaacggc tcaatttcgc ccatgaccgt gatccccagt aacgccgcca
    33241 gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt
    33301 tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga atgaccggag
    33361 actctccttt ccagaagtag ccatgatgca caatgaccgc atccgcgccc agacgcactg
    33421 cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc accgtctctt
    33481 tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt
    33541 cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta ttctgatagc
    33601 tacaccgtta acatagcgtt acctcgcctg ttcgtcgata gccaatcttt tgacatatta
    33661 agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt cagctaatgt
    33721 atatttatgc aatcagacag catattgcat aaataatcag atctgtatca aagttttaac
    33781 tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca attgtggaat
    33841 tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat
    33901 ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc tcaccaatca
    33961 actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt cgctggtgta
    34021 tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc gcatggcggt
    34081 gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca
    34141 tccgtcattc ctctatctgt ccctggcgat tatcgtttgt ggctatggtc tgtttaaatc
    34201 caatgtcagt tgcctgctcg gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg
    34261 tttctcgctg atgtatgcag cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg
    34321 ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg tgggtatgat
    34381 cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc acacataccc gcggcgttaa
    34441 taaaaaagta ctgcgtgcga caaactttct cctgcctaac tggggatggc tgctggttct
    34501 gctggtggca acgcctgcgc tgattaccgt attgttctgg aaagagtggt cggtatacgc
    34561 cttaattgtc gcgactatta ttggcctggg tgtactggca aaaatttatc gcaaagcaga
    34621 aaaccagaaa cagcgtaagg agctggggct gattgtgacg ctcaccttct tcagtatgtt
    34681 gttctgggcc ttcgcacaac agggcggcag ctcgattagc ctttatatcg accgcttcgt
    34741 taaccgcgat atgtttggtt ataccgttcc gactgctatg ttccagtcga ttaatgcctt
    34801 cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa
    34861 tcgtaccgtg cgcatctggg gaaaatttgc tcttggtctc ggcctgatga gcgccgggtt
    34921 ctgcattctg accttaagcg cccgctggtc ctcaatgtat ggtcactctt ctctgccact
    34981 gatggtatta ggcctggcgg tgatgggctt tgcggaactg tttatcgacc cggttgccat
    35041 gtcgcaaatt acgcgcattg aaatccccgg tgtgaccggc gtattaaccg gcatttatat
    35101 gctgctttcc ggcgcgattg cgaactatct cgcgggcgtg attgccgatc agacatcgca
    35161 ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata ttgaagtatt
    35221 tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga tttggctgta
    35281 tcaggcgctg aaattcagaa accgcgcgct ggcgctggaa tcttaatagg ccagcaccat
    35341 acctgataaa acgctcgctg cgttttatca ggctcctttc ctcgcttctt cgtacgctgt
    35401 cagcgttcgc agccttgcct ctttgtgatc aactatcggt tgcggataat ccagcatcac
    35461 acctgctttc tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag
    35521 ctccggtaac catcggcgga taaactcgcc ctcacggtca aatttctcgc cctgagttat
    35581 cggattgaaa atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca
    35641 gccaccgtta ttagctgcca aatcaccatc aatcagctgc gacatgaaat atcgctcgcc
    35701 ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg
    35761 gttatgcatc cagccagtac tgttaagctg acgcatagcg gcatcaacaa tcgggtatcc
    35821 cgttttgcct ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc
    35881 cgtccaggca ataaacggac aatgtttaca caacgagggg taatacgtca tcagatgacg
    35941 gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc
    36001 ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc
    36061 caggctggcg gacaaacgac tggtgccttc cactgccgga aaatctcgtt gttgctcata
    36121 ttctccggca ccgttctggc aaaactggcg taattgcgca atcgccgctt tttcttccac
    36181 cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg
    36241 ctctatcgat ccgctactac gaacttttgg cgcagcgaca cactccggca tcccttcccg
    36301 cagccgtttc agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattacc
    36361 ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt
    36421 acgcagcgct ctttcaactt ccacatcccg cgcccgctca ttcacttcat actgatagtt
    36481 ataaaacagg tgggtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc
    36541 gacaaagtca tccacttcac ggaacaataa aggaatacct ttttccgcaa gcgctatttg
    36601 tagcccattc agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc
    36661 ccactggcgt ggtgtagcga tatataacgc cagcacgcgt gcagacgaat tgcggcaggc
    36721 ggcagccagt gcgagattat cgtgcagacg taaatcctgg cgaaaccaga ccagatgggt
    36781 aatcataaaa ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc
    36841 tgccctgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc
    36901 gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgccgccaa ctgctggcgt
    36961 tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga
    37021 tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc gttggtagta ctggttgtaa
    37081 aaatcatcca gattttgcca ctggtgaata tcagccacga acgggccaag tttgcggtac
    37141 tccggctgag aatgcgccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc
    37201 ccgcgcgtta agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt
    37261 agggtgctgt catcaaatcg ttgctgattc attactgctc ctgaacggtt tacaggagta
    37321 agtgtagacg ttgagaaatg atatttgtga agttaatcct gttgtagcag gacaaattta
    37381 cgaaagcctg ctccccgcaa ggactgacgt caggtagttt ctaaccatag ctgccctttc
    37441 tattcaaacg tgaaatcaat aagctcatca gggacatcgc aaacaatatg aagacgggtt
    37501 tcttctctgc cgtgactctt ttcactaatt gcgtaactgt catgcttggg attatttaat
    37561 tctttcagcg gaaatttttc ctcgaaggct ttatttagcc gcccctggtt tccttttaca
    37621 gcgaataaat aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc
    37681 atcgcatcgg ttgtgatgat ttttccttta atatccagca tgttaagaag ttcagggata
    37741 gctgtgatct cattggattt cttatccgtc ttgatctgcc cgatgaccag actgtgcatt
    37801 gttgagaacg cac
//
LOCUS       DAHBHC010000048        36751 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHBHC010000048 DAHBHC010000000
VERSION     DAHBHC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.048"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(65..2494)
                     /gene="torZ"
                     /locus_tag="M2A35_002847"
     CDS             complement(65..2494)
                     /gene="torZ"
                     /locus_tag="M2A35_002847"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCB7199124.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(2519..3619)
                     /locus_tag="M2A35_002848"
     CDS             complement(2519..3619)
                     /locus_tag="M2A35_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCB7199125.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="M2A35_002849"
     CDS             complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="M2A35_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCB7199126.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(4767..5333)
                     /locus_tag="M2A35_002850"
     CDS             complement(4767..5333)
                     /locus_tag="M2A35_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCB7199127.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5549..7282
                     /gene="argS"
                     /locus_tag="M2A35_002851"
     CDS             5549..7282
                     /gene="argS"
                     /locus_tag="M2A35_002851"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCB7199128.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            7459..7947
                     /locus_tag="M2A35_002852"
     CDS             7459..7947
                     /locus_tag="M2A35_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCB7199129.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="M2A35_002853"
     CDS             complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="M2A35_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCB7199130.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="M2A35_002854"
     CDS             complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="M2A35_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCB7199131.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLLPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESSLKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLESLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="M2A35_002855"
     CDS             complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="M2A35_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCB7199132.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="M2A35_002856"
     CDS             complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="M2A35_002856"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCB7199133.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="M2A35_002857"
     CDS             complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="M2A35_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCB7199134.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="M2A35_002858"
     CDS             complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="M2A35_002858"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCB7199135.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="M2A35_002859"
     CDS             complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="M2A35_002859"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCB7199136.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(14870..16471)
                     /gene="tap"
                     /locus_tag="M2A35_002860"
     CDS             complement(14870..16471)
                     /gene="tap"
                     /locus_tag="M2A35_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCB7199137.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IIGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(16517..18178)
                     /gene="tar"
                     /locus_tag="M2A35_002861"
     CDS             complement(16517..18178)
                     /gene="tar"
                     /locus_tag="M2A35_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCB7199138.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNAEDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQESALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSTFRLAASPLTNKPQTPSRPASEQPPTQPRLRIAEQDPNWETF"
     gene            complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="M2A35_002862"
     CDS             complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="M2A35_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCB7199139.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="M2A35_002863"
     CDS             complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="M2A35_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCB7199140.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVWGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(20816..21742)
                     /gene="motB"
                     /locus_tag="M2A35_002864"
     CDS             complement(20816..21742)
                     /gene="motB"
                     /locus_tag="M2A35_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCB7199141.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(21739..22626)
                     /gene="motA"
                     /locus_tag="M2A35_002865"
     CDS             complement(21739..22626)
                     /gene="motA"
                     /locus_tag="M2A35_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCB7199142.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="M2A35_002866"
     CDS             complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="M2A35_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCB7199143.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="M2A35_002867"
     CDS             complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="M2A35_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCB7199144.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            24464..24892
                     /gene="uspC"
                     /locus_tag="M2A35_002868"
     CDS             24464..24892
                     /gene="uspC"
                     /locus_tag="M2A35_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCB7199145.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(24899..26323)
                     /gene="otsA"
                     /locus_tag="M2A35_002869"
     CDS             complement(24899..26323)
                     /gene="otsA"
                     /locus_tag="M2A35_002869"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCB7199146.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(26298..27098)
                     /gene="otsB"
                     /locus_tag="M2A35_002870"
     CDS             complement(26298..27098)
                     /gene="otsB"
                     /locus_tag="M2A35_002870"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCB7199147.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(27265..28251)
                     /gene="araH"
                     /locus_tag="M2A35_002871"
     CDS             complement(27265..28251)
                     /gene="araH"
                     /locus_tag="M2A35_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCB7199148.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(28266..29780)
                     /gene="araG"
                     /locus_tag="M2A35_002872"
     CDS             complement(28266..29780)
                     /gene="araG"
                     /locus_tag="M2A35_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCB7199149.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(29850..30839)
                     /gene="araF"
                     /locus_tag="M2A35_002873"
     CDS             complement(29850..30839)
                     /gene="araF"
                     /locus_tag="M2A35_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCB7199150.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            31636..32139
                     /locus_tag="M2A35_002874"
     CDS             31636..32139
                     /locus_tag="M2A35_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCB7199151.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(32218..32469)
                     /locus_tag="M2A35_002875"
     CDS             complement(32218..32469)
                     /locus_tag="M2A35_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCB7199152.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(32584..32670)
                     /gene="azuC"
                     /locus_tag="M2A35_002876"
     CDS             complement(32584..32670)
                     /gene="azuC"
                     /locus_tag="M2A35_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCB7199153.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            32933..33256
                     /locus_tag="M2A35_002877"
     CDS             32933..33256
                     /locus_tag="M2A35_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCB7199154.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            33427..33924
                     /gene="ftnA"
                     /locus_tag="M2A35_002878"
     CDS             33427..33924
                     /gene="ftnA"
                     /locus_tag="M2A35_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCB7199155.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(33962..34201)
                     /locus_tag="M2A35_002879"
     CDS             complement(33962..34201)
                     /locus_tag="M2A35_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCB7199156.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            34392..35603
                     /gene="tyrP"
                     /locus_tag="M2A35_002880"
     CDS             34392..35603
                     /gene="tyrP"
                     /locus_tag="M2A35_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCB7199157.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(35665..36330)
                     /gene="yecA"
                     /locus_tag="M2A35_002881"
     CDS             complement(35665..36330)
                     /gene="yecA"
                     /locus_tag="M2A35_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCB7199158.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(36526..36612)
                     /locus_tag="M2A35_002882"
     tRNA            complement(36526..36612)
                     /locus_tag="M2A35_002882"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36576..36578),aa:Leu,seq:taa)
     gene            complement(36625..36698)
                     /locus_tag="M2A35_002883"
     tRNA            complement(36625..36698)
                     /locus_tag="M2A35_002883"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36664..36666),aa:Cys,seq:gca)
BASE COUNT         8979 a         9762 c         8672 g         9338 t
ORIGIN      
        1 tagcgcatca ggcagtttta cgtttgcata acctcagcgc ccgtttccgg gcgctattca
       61 cttcttatgc ctgaatagct ggctgatcaa acgccgttaa cttcggcgca ttgcccgtat
      121 atttttcgat atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg
      181 ggatatccgc cgttaacacg ttcgcactgc cgtttttaca caagccattt tccagatctg
      241 gccatgcacc ttcatgcacg cataccacgc cttttttgat cccgtcagtc accaccgcgc
      301 ctgtcagaat ctgaccacgt ttgttccaca cacgcaccag atcaccattc gcaataccaa
      361 agcgagcagc atcttcggtg tgaatagtga ttggttcacg atctgcaacc gcatattttt
      421 tacgcagttc cgcatagtta agctgactat gtaaacggtg tgccggatgt gcggtcagaa
      481 gctgcaactg cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttgggt
      541 gtgccgggca atccttatag ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc
      601 cgcttggcgt acccagcgca tttttcaccg gatcggcgcg gaaatcagca taacgaacgt
      661 actgttcgtt cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaaggcat
      721 taaacatcgg catagtcacg cgttgcgcac gagcaccttt ctgagcggca tcatagaaaa
      781 atttcagcca cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttca
      841 gtaattcagc aagatcggca aatacgtcaa aatcgttacg cgcttcaaat tgcggagcaa
      901 cagcctgctt catcggcaca atatgctggt tgctgtaatc accggtcatc gtcaagtcat
      961 tgcgctcaaa cgatgtggtg atcggtaata cgatatctgc atgtttagca gctgcggtcc
     1021 agtagcattc agaaacgacg atcatctccg gtttctgcca tgctttgatc agacgattag
     1081 tatcctggtg atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat
     1141 acgtttgttc tttaccgtta tgctgatatt tgccaccagg attttccagc gcatcgacga
     1201 tacgggcaac aggaatcgcc gtcattccgc catcatcagc ggcctcgctg gcctgtccgg
     1261 caatcgccgc agacatttcc ggcagcacgc cgccaacacg cgtcggattg ccaccgttgg
     1321 agtaatgata agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg
     1381 ccagcgtcac cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc
     1441 cagccatcag cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt
     1501 ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt cggagtatta tcgctcttac
     1561 ctgtcagata ctcttcaaac tgcggatacc cggtggtgta tttttcaagg aacactttat
     1621 cgtgtttacc ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc
     1681 ccatattcgg cgcgatccag gtagcattat caccaaagaa ctcgatggtt tcggagcgga
     1741 taggatcaat ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc
     1801 cttgctcatc ggtactgctc caggcaattt ttaaggtatt cagcgggttc atcccccaca
     1861 gcaccacagc ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct
     1921 ctacagaacc gaccacatgc ggcatgatca cctgtgcagc cccggttgaa taatcgccgc
     1981 tatgcccgga ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat
     2041 gcaacacgcc agaagagcgc cagccgtatg atccggcaaa aatagcagat ggtccgttag
     2101 ctttgcgaat gcgatcgtgt tgctcatgaa tcagttttaa tgcttgctcc cagctcacct
     2161 gtacataagt atcttcgcca cgacctttcg ccggttgcag tggattatcg agatagcttt
     2221 ttcttaccat cggatgctga atacgtgccg tggtgtgtac ctgatccgcc gccgtagact
     2281 gtaaggagtt cggtatggtt ttcgccagcg cgcctgttga agaaacaatc ttgccgtcct
     2341 tcacttctac gttcatcgct ccccaacgtc ccgcggtgag gattttaccg cccttctctt
     2401 ctgcccatgc ggggagcggt gctgccgatg tcaccaccag cgctccagcg gcaataccgc
     2461 tgtgtttaat aaattcacgt cttgttaatg tcataacttc ctccctgatc aacgaggatc
     2521 actgtttctc ggtaatatct ttggcgttgt actggaaata ccgcgttaaa atgtccagtt
     2581 cgttttcgct catgctggtt cgcgcaccca ttcctttggc aatggacggc catgcattga
     2641 cggtgtaatg gtcagcggca ataggcgcat ggcaaccagc gcaataggta tcgtcaagtt
     2701 tttcagcgta ttgccatagc ggtttacggt ccgctaatgc gggatcggta agcgcaccct
     2761 gtaaagacgc ctgacgccat tgattgccgt attcgtcagc ctgccattcc ccgtttaccg
     2821 tgagcgcctt gataccttct tcacttaatg tggctagcgc cagccgttga cctgccgcca
     2881 ggtagagcgt gttttcactg ccctgcattt gataaccctg caacagaacg atcggctgtt
     2941 tgccactggc atcaacgacg gtgagatcgg taccaggatt cacggtagcc agctcgccta
     3001 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
     3061 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
     3121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
     3181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
     3241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
     3301 cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg
     3361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt
     3421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
     3481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
     3541 gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa
     3601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgta
     3661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
     3721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg
     3781 ctatttagaa tatatattta tcagttttag caatttaaat cccataatca atgtgaatat
     3841 aaaaaatatt attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
     3901 cgttcgtttt ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct
     3961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     4021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat
     4081 actcgtccgc gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     4141 aggcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     4201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     4261 taagttccat aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta
     4321 cccgggcaat gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat
     4381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     4441 gtggcatatc gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta
     4501 attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     4561 aatcaccacc gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg
     4621 atttcagtac ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     4681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     4741 ccagtaatgc catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca
     4801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     4861 ggtaatcgct catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg
     4921 agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc
     4981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     5041 agctccacaa tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     5101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg
     5161 cgacgccagc gttcagcagt ggcgttttga tggcaacgca aagaaatgtg atcggcagtc
     5221 agcggagtga tattcaaccc cagacggagg gaaagttcat ctaatgcgtg ggtaaatcgc
     5281 ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact
     5341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat
     5401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     5461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     5521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     5581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     5641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     5701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     5761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     5821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     5881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     5941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     6001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     6061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     6121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagttcgccg agcgtgcacg
     6181 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     6241 ggtcgacatc accatgacgc agaaccagat cacttacgat cgtctcaacg tgacgctgac
     6301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     6361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     6421 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     6481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaatcac tgcatgccga
     6541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctaatgcagg catgggcgat
     6601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     6661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     6721 cgatctgctg gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     6781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     6841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     6901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     6961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     7021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     7081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     7141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     7201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     7261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     7321 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     7381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     7441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     7501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     7561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     7621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     7681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt
     7741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     7801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     7861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     7921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     7981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     8041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     8101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     8161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     8221 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     8281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     8341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     8401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     8461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     8521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     8581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     8641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     8701 cctgtagtaa caaactttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     8761 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     8821 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     8881 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     8941 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     9001 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     9061 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     9121 gttcttttag cgaactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     9181 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
     9241 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     9301 gatccatatt gtcgcgaatg tgcaccactg gcagcagaaa tcccatctcc tgggcaaatt
     9361 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     9421 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     9481 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg acaggcgctt
     9541 tttgttcgcg tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     9601 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     9661 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     9721 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     9781 ccaccagacc gtcgccaatg gtcaatagtg tataactttc cgccgcgtgt cccatgctca
     9841 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     9901 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     9961 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
    10021 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
    10081 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
    10141 gaatgacaaa cactacgata ccgatagcga aattgccacc aacgaggaag tgaccgaatg
    10201 cttcgaccac cttcccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
    10261 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
    10321 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
    10381 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
    10441 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
    10501 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
    10561 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
    10621 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
    10681 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
    10741 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
    10801 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
    10861 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
    10921 cgccgtgcag cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg
    10981 ctttgtttga actcatcacg aatatcctgc cgtgacatac gcagcttttt caggtggctg
    11041 aatatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
    11101 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccatc
    11161 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
    11221 atggttttca aaattgcttt aagcaactcc gcgccagtct gagccgagaa catccgttta
    11281 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
    11341 agccctccca gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc
    11401 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
    11461 tcattgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
    11521 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
    11581 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
    11641 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
    11701 ttaagcgtga atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag
    11761 gcgggattta gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc
    11821 gtcatcgcct tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat
    11881 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacgccggct
    11941 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
    12001 tcctgttccg ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg
    12061 acatccatca tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg
    12121 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt
    12181 cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
    12241 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
    12301 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
    12361 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
    12421 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
    12481 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
    12541 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
    12601 caccacatag ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc
    12661 tgcagtcacc attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag
    12721 caattccagg ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
    12781 tgcctgcaac ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc
    12841 cagctcttta agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
    12901 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
    12961 gcactaattt ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca
    13021 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc
    13081 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc
    13141 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
    13201 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
    13261 taatttgcgc cactacgcga cagctccata tgccgatcgc ccggcgcaat ataggcatgc
    13321 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
    13381 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gttgggtaat taacaatgcc
    13441 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
    13501 gcaccaatcg caatcagttt ttcagaactc aacaacggac ccgccttcag cgttgtcggt
    13561 gctgacaatg gcttatgtgc tgcaaggctc gcctttgctg ccgtacgcac cttttcagca
    13621 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
    13681 tctattgccc ccagctccag cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa
    13741 gaaaccatca caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg
    13801 tccatccgcg gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag
    13861 tcacgcgcga ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg
    13921 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
    13981 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
    14041 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
    14101 gcggaacaaa gcggcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt
    14161 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttctcc agtagattca
    14221 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
    14281 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
    14341 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa
    14401 acactttcca gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
    14461 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
    14521 gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg gaaaaatgcc gtcagattcg
    14581 tggtcagcga attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt
    14641 tcagataatg accgaaatcc gttagtccca gcgaacgcaa acgacgaacc agtcggttgt
    14701 aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac
    14761 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    14821 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    14881 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa
    14941 ataccgccac acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg
    15001 ccgcttcttc taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct
    15061 gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc agaggcaatt tctccaataa
    15121 tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca
    15181 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    15241 ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    15301 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    15361 tctggaaagc aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg
    15421 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    15481 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    15541 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    15601 tacgacttga gagatcgtta ttgcctgcga caatctccgc gatcccaatg tgcatctcat
    15661 ggcttccctt acgcacatca ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    15721 tggcaaaaat ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac
    15781 ccgcagcaat gctgtcaaaa tggctaccaa taatggccag tggttgaaca atcatcttgc
    15841 gcgtccacca cagcgcactg ctgatgtaga ttgccgcaac aataatcatg ctgataaaca
    15901 ccagcgccga aatctgatag ttacgctgac tttgcgcact ggcggcttcc agcacatggt
    15961 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg
    16021 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    16081 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caatgccctg acgtgctcgt
    16141 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    16201 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc
    16261 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    16321 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    16381 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    16441 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    16501 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    16561 gtggctgtgt cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    16621 gtgggctggc tgccagacgg aacgtggaaa ccgcttgcgt taaacgactc gcctgttctt
    16681 ccagcgcagc ggcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    16741 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    16801 ccgatgcaat ctcgcccata atgtcagtca cacgagtgac agcattgacg atattgttca
    16861 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgtgag acggagtctt
    16921 caatgagggc tttgatctct tttgccgcct gggcgctacg actggcaaga ttacgcactt
    16981 cacccgccac cacggcaaaa ccacggccct gttcacccgc acgcgcggct tcaaccgcgg
    17041 cattcagcgc gaggatatta gtctggaagg caataccgtc gataacgctg ataatgtcgg
    17101 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    17161 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    17221 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    17281 gcgcggattc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    17341 gggtaccggc atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag
    17401 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    17461 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    17521 gcggagtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    17581 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcttctgcg ttgtcagtga
    17641 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    17701 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    17761 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    17821 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgcgg
    17881 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat
    17941 tggaggaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    18001 ttaaatccca ggttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    18061 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    18121 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    18181 ggcaccttcc tgaaaacaag ttaatctcgt tatcggcaag aaggggggaa actttattgc
    18241 tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cggggtcatt
    18301 tcaattgagg aaatcgggat aattacgcca cttctgacgc cgcgctatct aacagcgcca
    18361 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatccgg tcgcccagtg
    18421 cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc aaactctggt gccggacgaa
    18481 tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc aaccacgatg ccgaccaccc
    18541 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    18601 tgaacttaat tcgtaagtca acaatcggca caataacgcc gcgcagattc gtgacgcctt
    18661 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    18721 ctttcaggat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    18781 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    18841 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    18901 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    18961 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgctgac caattaattg
    19021 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    19081 ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    19141 ttcaccccac acttccagca cccgctcgcc gccggccagt ggatgcagat cggcttcacg
    19201 gggttgcagt gattccataa cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    19261 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc
    19321 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    19381 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctctgccg tggagaagcc
    19441 tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc
    19501 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgccccat cgtcggtcac
    19561 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    19621 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    19681 caggtgggtc agcgggtcga taatgcgttc tatcaggctt ttgtcgagtt cagtagaact
    19741 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg
    19801 gggatagcga ctaaaaacat attccatcgg catcatgcga atcgacatca ctgattcctg
    19861 caaatcacgg gcgttacgtt gtaactgccc catgctggtt atcaaatcac catgattaac
    19921 cgggtccagt tcgctggaac gctgggcaag catggactgg gtgataacca gctcgccgac
    19981 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcattgct
    20041 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    20101 cactggtggg gtggatattt ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    20161 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    20221 tgccgagagc gaatccgccc ctttcaccac gtcagttaac gttgtcagat gtcccagttc
    20281 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    20341 ctgactgcga ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    20401 tgcggatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    20461 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    20521 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    20581 tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    20641 aaaagttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    20701 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    20761 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    20821 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    20881 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    20941 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    21001 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcta tccaaccctc
    21061 cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc cagttgctat
    21121 atcctttctc accgctggcg taggggaaat catcggtatg acctgaaagg ctaatacggt
    21181 tgggaatacc gttcagtaca ggcgcaatgg cacgcagaat gtcgcgcata tagggttcga
    21241 catcggcact gccggttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    21301 gaccttcctg gaccagatcg attttgagat ggggacgtaa cgcccgcagt ttcggatcgg
    21361 actctatcaa ctggtcgaga tcaccccgca atttccgcaa tcgactttgc tccatgcgtt
    21421 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    21481 caccaccgcc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    21541 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    21601 agatcagcca catcaccaga aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa
    21661 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    21721 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    21781 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    21841 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagaaa gcagagtgac
    21901 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    21961 tggggaaata aatccgtaag ccaataaaat gccgaggaaa gtccccacca tcgcatgtgc
    22021 gataagcgca cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    22081 cattacagcc gcgacaatac caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt
    22141 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gggcttcgat
    22201 ttcgaaggtg ttcatatgac cgctgataat cagacgcaga taatcgacga taaaatcaag
    22261 catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    22321 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    22381 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    22441 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    22501 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    22561 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    22621 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    22681 cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc
    22741 tcaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    22801 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct
    22861 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    22921 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    22981 taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc
    23041 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt
    23101 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    23161 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc
    23221 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    23281 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    23341 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    23401 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    23461 atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    23521 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    23581 cgaaacatag cggacgcttt gtcctgaaca atcaaacgct gtgcaagtag taaatatgac
    23641 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    23701 aaccaacttt atttttatgc ggtttcaccg caccccgtga tgtcgccggg aagccccggt
    23761 aaaaaataat tagcattaga atagttgcga taagctgcaa taagcagaac cacctttttg
    23821 gtttaatatg tccttacaaa tagaaatggg tctttacact tatctaagat ttttcctaaa
    23881 tcgacgcaac tgtactcgtc actacacgca catacaacgg aggggggctg cgattttcaa
    23941 taatgtgtga tgcagatcac acaaaacact caattactta acataaatgt ataagtcata
    24001 cttttgtttt gggtgtattt caatctgtta aaaagttttt cgctacgcta gcaagcaaaa
    24061 atgaaacagg aataatcgaa atgggatgtt gcgcacactt aaaataactc accgtaaata
    24121 atcatctgct ataaataatc actttcatgc aataccagat aagctatttt taaacagaca
    24181 cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa caatcaactt aatgttaatg
    24241 aaatgtttta catatattaa ccacttgttt aatgcggaga atcagtacac tattttctta
    24301 taattacatt tgaaattata tgatcaccgg gagtgattga ttaattgtct ttactaataa
    24361 tcagactaat atttacctgt ttgaccgagt tgggattgcg tcgtttctcc attaggagta
    24421 aagctttaat gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat
    24481 tcttgtcgct gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat
    24541 cgccaggcca gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta
    24601 caatcaatta gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca
    24661 aagctttctt gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc
    24721 ctacggtgag ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt
    24781 gatttgcggt aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt
    24841 tattgcctca agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct
    24901 acgcaagctt tggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa
    24961 ctatctgctt taggtcgcta atgaagcact cctgccaatg gttaatatcg tttttcacga
    25021 taacgtccag catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac
    25081 gatccagcgc agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta
    25141 actcgtttgc cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct
    25201 gagcagcaac atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc
    25261 ccacgtcaga gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat
    25321 aataaagcgg cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt
    25381 cgagctgatg acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct
    25441 gggtataacg aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga
    25501 gaaaacgctc tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta
    25561 cgtttttcag ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt
    25621 tggctatttc tttcggttca atgccgatcg ggtagacttc tgttcgaaat gctttgcccc
    25681 aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa agacaatcca
    25741 ggaacgccag acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt
    25801 caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat
    25861 gcagaaagaa accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaatggca
    25921 acaggtgata atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat
    25981 ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca
    26041 gatcgagccg ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt
    26101 cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct
    26161 tttttagcgg ttgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg
    26221 cggctttcag tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt
    26281 ctggtggtgc aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta
    26341 ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc
    26401 acacccgcca gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg
    26461 cccagtcggt taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg
    26521 ggcgttcgcc cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg
    26581 gtacctctcg gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag
    26641 atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga
    26701 taatgcagcg caaaagccat ccctttcgcc tccagctccg cgccgggata ctgagcgatg
    26761 actgtatgca gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt
    26821 gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa
    26881 ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca
    26941 tcacttgcgg ttgccagtag ctgtagtcct tgcagaatat tgtcaggcac gacgacctga
    27001 tcgggatgcg gtttgatttc cgccagcgtt ccatcaagat caaaaaacca ggcatatttc
    27061 gcggatagtt caggggtttc ggttaacggt tctgtcaccc ggctctcctc cttctctttt
    27121 cttattgttt tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg
    27181 tcgccatttg caatgaagaa cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac
    27241 gttaaattgt tgtggaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacggtc
    27301 gaagatcacc gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat
    27361 attaagcagg ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata
    27421 tgagattttt ccgatgccac ctttcagaga aacgccacct aaaacgcagg cggagataac
    27481 aataagctca taaccaatcg acgtcattgg ctgcccacta gtcatacgtg aagccagaat
    27541 aattccggct atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgaac
    27601 aaccggtaca cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg
    27661 accaaaggtg gttttattca gcaacaaacc aaagataatc agacacgcga cggtgagcca
    27721 gattggcgca ggcagaccga accagttagc gtaaccaagg gcaaagaagc tttcatcttc
    27781 gataccgacc gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt
    27841 tgccagtgtt gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag
    27901 gccacagaga atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag
    27961 gttgataacc accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc
    28021 aaagtcaccg gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat
    28081 tgccaggccc aaccctttca tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca
    28141 ggcgataaag agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa
    28201 gctgaatgac gacttaggtg cgccagaccc cgatgtagaa acagaagaca tcatactctc
    28261 cttactcagg caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca
    28321 tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg
    28381 tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc
    28441 gccgccagcg cataaatcac gttgtaaatt tcatgcttag caccaacatc aatgccgcgc
    28501 gtaggttcat ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct
    28561 ttttgctgat ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg
    28621 ttgagcgaac gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa
    28681 ccgccgagca catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg
    28741 ggaataatgc cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg
    28801 tggctcggtt tacgaatatc gatcggctgt tggtcgatat aaacctgacc tgcggtgatt
    28861 tgcgtcccgc caaacaagcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac
    28921 agcccaacaa tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct
    28981 ttcacagcat caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata
    29041 tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata
    29101 tcggtaaagg ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca
    29161 aatatttctt ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca
    29221 cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca
    29281 aaggcgataa ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga
    29341 ccaatggaga gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt
    29401 tgtaaacccg cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc
    29461 tggccgagat agatgttttc cgcgacggtc atttccggca cgagatgtag ttcctggtaa
    29521 ataatcgcca cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca
    29581 ttaatcacta cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt
    29641 gattttcctg cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa
    29701 ctaatatccg tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga
    29761 tacggggtag actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc
    29821 atcatgcaga ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt
    29881 tcttctttaa agttgtcacg cgtgatcagt accacgtcgg taacttcggt aaattttggc
    29941 ggttcaacgt cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg
    30001 tccgggcttg gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc
    30061 accgcatcca caccgttaat gccaatgccg atgatatcgg ccgctttaaa gccctgacct
    30121 tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt
    30181 ttaacttccg gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg
    30241 ttagatttgg taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca
    30301 tccatagatc ccgtagtacg gcggcgggcg gtatccagtt cgttggcggt aatcgccatc
    30361 accgcgcttt ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctggccc
    30421 tgacgttcgc caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta
    30481 cctttggcgt taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct
    30541 ttcgcgacga tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg
    30601 ccactggcag ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc
    30661 ttaataacct caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg
    30721 aaccacggct cttccggttg cttcaccaga aaaccgagct tcaggttctc cgccatagcg
    30781 gattgtgaca taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg
    30841 gttctctcca gctttagtgt cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt
    30901 aatcggaaaa caaagcatta ccttttaact aaaagataag tgactgtgtt gacatagttt
    30961 tagcgagaaa ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac
    31021 gtgcataaat ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga
    31081 atgcacagca gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat
    31141 gcgggatgtc ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgaa
    31201 atggtgtaat aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgccattaa
    31261 ttatcactgt gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga
    31321 aaaaacgttt tataaaatca tcacttcatc atgaattcaa attcattgat taatatcaac
    31381 aagatacaaa aagcgctatc attaaaattc attgcagtta cattgatttc atcaatgaaa
    31441 tgtaaaaata tataaacttg atgatttaag cattttctta tacccgttca gacgttattc
    31501 ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa
    31561 ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat
    31621 ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac
    31681 cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt
    31741 ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg
    31801 cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt
    31861 cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag
    31921 caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca
    31981 accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa
    32041 accattcttg atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac
    32101 caacatgttc tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat
    32161 tgcgccgatg aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta
    32221 atgggattca ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc
    32281 ccgcatggtg accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg
    32341 actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg
    32401 tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg
    32461 ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc
    32521 ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt
    32581 ttattatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa
    32641 catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt
    32701 gttattctct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac
    32761 aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat
    32821 ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc
    32881 gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact
    32941 tctgattctg actctttcat taataacgct ggcggggtgt acggttactc gccaggccca
    33001 tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt
    33061 gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca
    33121 ggaaggctat acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc
    33181 gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt
    33241 ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc
    33301 ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg
    33361 caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc
    33421 actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg
    33481 tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt
    33541 gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt
    33601 gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct
    33661 gaatattcct cacttgatga attattccag gaaacctata aacacgaaca attaatcacg
    33721 cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat
    33781 ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt
    33841 gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc
    33901 tctaccctcg acacacaaaa ctaatgctct caggcggcag gttttctgcc gccgtttcaa
    33961 atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa ggtataaatt
    34021 tgccttttgc tgccagaaac gctaccagct ctcccgcagt catcccttct gccgagcagg
    34081 tgtgaaaacg tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga ctggcatgcg
    34141 tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca
    34201 tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag
    34261 cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg
    34321 acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga
    34381 cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa
    34441 ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt
    34501 taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg
    34561 tgtaccagca tgttccggca gataccggtc ttggcacgct ggcaaaacgc tatctgggac
    34621 gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg
    34681 catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt
    34741 caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg
    34801 ttggaacatc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc
    34861 tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc
    34921 cgttgcaaca ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc
    34981 acggtagcgt gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg
    35041 tgtttataac cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc
    35101 ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg
    35161 ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt
    35221 tatttgctga tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt
    35281 atctggctga tttatttcag cgttcaaata ccgttggtgg acggttgcaa actggtgcaa
    35341 ttacctttct gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc
    35401 tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct
    35461 ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt gaaaggtggt cgtccggcgc
    35521 tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag
    35581 ggttgttacc agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt
    35641 aacgctgaca cccaatttga cctcttaatg caggcagcac tgcttaaatt tcttaccact
    35701 accgcacggg caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac
    35761 agctttttcc tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat
    35821 ggcatctacg ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc
    35881 ctcagtaccg tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga
    35941 ccagtcagaa agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag
    36001 ctcgctgcca tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt
    36061 cagacgctct gcggtatcgg ccatatgttg aaaagccaga ttcataaagc gcgtcatctc
    36121 tttctctgac gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg
    36181 ttccggttca atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc
    36241 cacatcaagg atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa
    36301 ctcactttcg tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca
    36361 gcacctatct tacatgccgg tccgtatcag agatactttt tgagtggctt tgctggtgat
    36421 taaaaattag ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt
    36481 ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg
    36541 gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc
    36601 caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg
    36661 atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc
    36721 aaacatccgc ttgacgccga tgtcttttaa a
//
LOCUS       DAHBHC010000049        36591 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHBHC010000049 DAHBHC010000000
VERSION     DAHBHC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.049"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..222)
                     /locus_tag="M2A35_002884"
     CDS             complement(<1..222)
                     /locus_tag="M2A35_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HCB7199159.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIG"
     gene            complement(358..465)
                     /gene="rprA"
                     /locus_tag="M2A35_002885"
     ncRNA           complement(358..465)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="M2A35_002885"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(651..1763)
                     /gene="ydiK"
                     /locus_tag="M2A35_002886"
     CDS             complement(651..1763)
                     /gene="ydiK"
                     /locus_tag="M2A35_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCB7199160.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            2152..5208
                     /locus_tag="M2A35_002887"
     CDS             2152..5208
                     /locus_tag="M2A35_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCB7199161.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            5205..5615
                     /gene="menI"
                     /locus_tag="M2A35_002888"
     CDS             5205..5615
                     /gene="menI"
                     /locus_tag="M2A35_002888"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCB7199162.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5715..5903
                     /locus_tag="M2A35_002889"
     CDS             5715..5903
                     /locus_tag="M2A35_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCB7199163.1"
                     /translation="MSTQLDPTQLAIEFLRCDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6451..6819
                     /gene="sufA"
                     /locus_tag="M2A35_002890"
     CDS             6451..6819
                     /gene="sufA"
                     /locus_tag="M2A35_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCB7199164.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6828..8315
                     /gene="sufB"
                     /locus_tag="M2A35_002891"
     CDS             6828..8315
                     /gene="sufB"
                     /locus_tag="M2A35_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCB7199165.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            8325..9071
                     /gene="sufC"
                     /locus_tag="M2A35_002892"
     CDS             8325..9071
                     /gene="sufC"
                     /locus_tag="M2A35_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCB7199166.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            9046..10317
                     /gene="sufD"
                     /locus_tag="M2A35_002893"
     CDS             9046..10317
                     /gene="sufD"
                     /locus_tag="M2A35_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCB7199167.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPFLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            10314..11534
                     /gene="sufS"
                     /locus_tag="M2A35_002894"
     CDS             10314..11534
                     /gene="sufS"
                     /locus_tag="M2A35_002894"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCB7199168.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11547..11963
                     /gene="sufE"
                     /locus_tag="M2A35_002895"
     CDS             11547..11963
                     /gene="sufE"
                     /locus_tag="M2A35_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCB7199169.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            12112..13116
                     /gene="ldtE"
                     /locus_tag="M2A35_002896"
     CDS             12112..13116
                     /gene="ldtE"
                     /locus_tag="M2A35_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCB7199170.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(13180..13416)
                     /gene="lpp"
                     /locus_tag="M2A35_002897"
     CDS             complement(13180..13416)
                     /gene="lpp"
                     /locus_tag="M2A35_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCB7199171.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13727..15139)
                     /gene="pykF"
                     /locus_tag="M2A35_002898"
     CDS             complement(13727..15139)
                     /gene="pykF"
                     /locus_tag="M2A35_002898"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCB7199172.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMTSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAQKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15696..15905
                     /gene="fumD"
                     /locus_tag="M2A35_002899"
     CDS             15696..15905
                     /gene="fumD"
                     /locus_tag="M2A35_002899"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCB7199173.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            16361..16987
                     /locus_tag="M2A35_002900"
     CDS             16361..16987
                     /locus_tag="M2A35_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCB7199174.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            17008..19110
                     /locus_tag="M2A35_002901"
     CDS             17008..19110
                     /locus_tag="M2A35_002901"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCB7199175.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            19123..19761
                     /locus_tag="M2A35_002902"
     CDS             19123..19761
                     /locus_tag="M2A35_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCB7199176.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            19825..20493
                     /locus_tag="M2A35_002903"
     CDS             19825..20493
                     /locus_tag="M2A35_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCB7199177.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            20490..21275
                     /locus_tag="M2A35_002904"
     CDS             20490..21275
                     /locus_tag="M2A35_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCB7199178.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            21279..22091
                     /locus_tag="M2A35_002905"
     CDS             21279..22091
                     /locus_tag="M2A35_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199179.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(22103..23707)
                     /locus_tag="M2A35_002906"
     CDS             complement(22103..23707)
                     /locus_tag="M2A35_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCB7199180.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(23833..24138)
                     /locus_tag="M2A35_002907"
     CDS             complement(23833..24138)
                     /locus_tag="M2A35_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCB7199181.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(24246..24322)
                     /locus_tag="M2A35_002908"
     tRNA            complement(24246..24322)
                     /locus_tag="M2A35_002908"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24286..24288),aa:Val,seq:gac)
     gene            complement(24327..24403)
                     /locus_tag="M2A35_002909"
     tRNA            complement(24327..24403)
                     /locus_tag="M2A35_002909"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24367..24369),aa:Val,seq:gac)
     gene            24711..25967
                     /locus_tag="M2A35_002910"
     CDS             24711..25967
                     /locus_tag="M2A35_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCB7199182.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPV"
     gene            complement(26008..27381)
                     /gene="mdtK"
                     /locus_tag="M2A35_002911"
     CDS             complement(26008..27381)
                     /gene="mdtK"
                     /locus_tag="M2A35_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCB7199183.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTALVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            27596..28237
                     /gene="ribE"
                     /locus_tag="M2A35_002912"
     CDS             27596..28237
                     /gene="ribE"
                     /locus_tag="M2A35_002912"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCB7199184.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(28277..29425)
                     /gene="cfa"
                     /locus_tag="M2A35_002913"
     CDS             complement(28277..29425)
                     /gene="cfa"
                     /locus_tag="M2A35_002913"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCB7199185.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(29716..30927)
                     /locus_tag="M2A35_002914"
     CDS             complement(29716..30927)
                     /locus_tag="M2A35_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCB7199186.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            31040..31972
                     /locus_tag="M2A35_002915"
     CDS             31040..31972
                     /locus_tag="M2A35_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199187.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(31969..32994)
                     /gene="purR"
                     /locus_tag="M2A35_002916"
     CDS             complement(31969..32994)
                     /gene="purR"
                     /locus_tag="M2A35_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCB7199188.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            33293..33382
                     /gene="ynhF"
                     /locus_tag="M2A35_002917"
     CDS             33293..33382
                     /gene="ynhF"
                     /locus_tag="M2A35_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCB7199189.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            33548..34723
                     /locus_tag="M2A35_002918"
     CDS             33548..34723
                     /locus_tag="M2A35_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199190.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(34869..35450)
                     /gene="sodB"
                     /locus_tag="M2A35_002919"
     CDS             complement(34869..35450)
                     /gene="sodB"
                     /locus_tag="M2A35_002919"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCB7199191.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(35578..36393)
                     /gene="mepH"
                     /locus_tag="M2A35_002920"
     CDS             complement(35578..36393)
                     /gene="mepH"
                     /locus_tag="M2A35_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCB7199192.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTTSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
BASE COUNT         9365 a         8643 c         9554 g         9029 t
ORIGIN      
        1 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
       61 catagccaac agttgctcga ctacacgatc aggaatggcg catttgccat tttcccactg
      121 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
      181 ggcaaaaata tggcgaagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
      241 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
      301 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
      361 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
      421 aggggatttc catgcttata aatcaatatg ttgatttata accgtaagct aatagtaggc
      481 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
      541 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gtaagcaaat
      601 atcaattagc gggggttaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
      661 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg
      721 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
      781 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa caccagagag gatcaggatc
      841 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
      901 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccagt ccagtagagc
      961 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     1021 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     1081 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     1141 gccgccagca gtacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     1201 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     1261 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     1321 gtgccaatat aagggcggac tttcgccatg atcgccgtcc cccccatatc cagcaagttg
     1381 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     1441 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     1501 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     1561 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     1621 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     1681 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     1741 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     1801 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     1861 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     1921 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     1981 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     2041 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     2101 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     2161 cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
     2221 caacaaggtt ttaccggcga tacggcgaca agttatgccg atcgtctgac aatgtcgacc
     2281 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     2341 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     2401 cgtggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     2461 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     2521 gccggggtga taaaagatca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     2581 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcttct
     2641 ggtcagggat cgctggtcta tggcaaaacc tcggatcacg tacttggcgt acgtgcggtg
     2701 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgaattggc ggaaacgctg
     2761 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     2821 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     2881 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     2941 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     3001 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     3061 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     3121 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     3181 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     3241 cgagttaatg cactctgtgt gcggcttgat gagctgattg tcagccaaca ggcaggtgtg
     3301 attggctggc aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc gatgcgcaaa
     3361 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     3421 gatacctgtg taccgccgga acatctggcg gattatattg ctgaatttcg cgcgctgctc
     3481 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt tttgcacgtc
     3541 cgtccagcgc tggatatgtg cgatcctcaa caagagattt tgatgaagca aatctctgat
     3601 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     3661 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     3721 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     3781 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     3841 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     3901 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     3961 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgttt gttggcggat
     4021 cgcggcgttg atccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     4081 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     4141 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcgtgttc gacccagtgc
     4201 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     4261 tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg agagctacgc gccgctgatg
     4321 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     4381 gaaaaacata tcggcatggt tgatttgccg ctgctgtcgg tcccctcgct acaacaacaa
     4441 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaatctct caatgcagag
     4501 cagaaagcgc gcacagtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     4561 gtggtggcgg attttgtccg tctggtcgaa aaattaggtt tccagcctgt gttactgcca
     4621 ttttcgccaa atggcaaagc ccagcatatt aaaggttttc ttaatcgttt tgcgaagacg
     4681 gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc tgggtatgcc aatggtgggc
     4741 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     4801 cgtggcgagt ttaacgtctt actggcgaat gaatggctgg caagcgcact tgagtcacag
     4861 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaagttacc
     4921 gccttgccgg gtgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     4981 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     5041 cataaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     5101 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     5161 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     5221 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     5281 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     5341 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
     5401 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
     5461 atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggggtatgc aaaccgttgc
     5521 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
     5581 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
     5641 atattgcctt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
     5701 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
     5761 gctgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
     5821 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
     5881 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
     5941 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
     6001 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
     6061 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
     6121 gtagaaaccc ttaagcctgt gttgcacaga cttaagggtt ttcttatttc tgaacgctgc
     6181 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
     6241 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
     6301 cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag catgttttac
     6361 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
     6421 acgctgaaag cgatgaagtg aggtaaatcg atggatatgc attcaggaac ctttaaccca
     6481 caagatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgtgag
     6541 ctggtggcaa agcagccggg tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc
     6601 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
     6661 gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg
     6721 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
     6781 cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata
     6841 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
     6901 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
     6961 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
     7021 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
     7081 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
     7141 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
     7201 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
     7261 ttttcgactc agtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta
     7321 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
     7381 gcaccgtggt gccggggaat gacaacttct ttgccgcgct taatgcggcg gtagcctctg
     7441 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
     7501 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgtac cattctggtg gccgacgaag
     7561 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac
     7621 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
     7681 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
     7741 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtccgcga
     7801 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
     7861 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
     7921 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
     7981 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
     8041 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
     8101 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
     8161 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
     8221 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
     8281 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
     8341 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gccgttcgtc
     8401 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
     8461 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
     8521 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
     8581 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
     8641 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
     8701 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
     8761 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
     8821 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
     8881 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
     8941 gcattctcga ctacatcaag cctgattacg tccatgtgct atatcaggga cgaattgtga
     9001 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
     9061 aacagcagta acgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
     9121 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
     9181 catgaaaact ggaaatatac gccgctggaa gggctgacca atagccagtt tgtcagcatt
     9241 gcgggagaga tatccccaca gcagcgagat gccttagcgt taacgttaga cgctgtgcgg
     9301 ctggtatttg tcgatggacg ttacgtgtca gcactgagcg atgcgactga aggcagcggg
     9361 tatgaagtga gcattaacga cgaccgtcag ggggtacccg acgctattca ggcggaagtg
     9421 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
     9481 caacggccgg caaagccatt tctgttaatg catattaccc agggcgtggc aggtgaagag
     9541 gtcaacactg cccattaccg acatcatctg gatctggcgg aaggtgccga agcaacggtg
     9601 atcgaacatt ttgtcagcct taatgatgct cgtcacttca ccggcgcacg gttcactatc
     9661 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
     9721 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
     9781 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
     9841 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
     9901 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
     9961 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    10021 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcaga agtggatacg
    10081 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    10141 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    10201 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    10261 cttaaacagc aggtgctggc ccgaatcggt caacgactgc caggaggtgc aagatgactt
    10321 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    10381 cgctggctta tctcgacagc gccgccagtg cgcagaaacc aagccaggtg attgactccg
    10441 aagccgagtt ttatcgtcac ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    10501 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    10561 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    10621 atagttgggg caacagcaac gtgcgggcgg gcgataacat catcatcagt cagatggagc
    10681 accacgctaa cattgttccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    10741 tgatcccgct caatcccgac ggtacgctgc aactggagac gctacctaat ctgttcgatg
    10801 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatcccc
    10861 tggcggaaat gatcacgctt gcgcatcagc atggcgcaaa agtgctggtg gatggcgctc
    10921 aggcggtgat gcatcatccg gtggacgttc aggcgctgga ttgcgatttt tacgtattct
    10981 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    11041 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    11101 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    11161 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    11221 ccgagtatga acagaatctg atgcactacg cgctatcaca gctggaatct gtaccggatc
    11281 tcactctcta tggcccgcgg aacaggcttg gcgttattgc ttttaatctt ggtaaacacc
    11341 acgcctatga tgttggcagt tttctcgata actacggcat tgctgtgcgt accggacatc
    11401 actgcgctat gccattaatg gcctattaca acgtccctgc tatgtgtcgg gcgtcgctgg
    11461 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggactgcaa cgtattcacc
    11521 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    11581 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    11641 agcgtctgcc agaattacgc gccgaagacc gaagcccaca aaatagcatt cagggctgcc
    11701 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    11761 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    11821 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    11881 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    11941 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    12001 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    12061 gcgccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    12121 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    12181 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    12241 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg
    12301 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    12361 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    12421 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    12481 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    12541 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    12601 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    12661 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    12721 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    12781 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    12841 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    12901 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    12961 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    13021 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    13081 ccagagcaag ggaatatgtt acgcgcgacg cagtagcggt aaacggcaga caaaaaaaat
    13141 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    13201 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    13261 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    13321 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    13381 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    13441 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    13501 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    13561 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttagata aagtgagatt
    13621 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    13681 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    13741 cagatgcggt gttagtagtg ccgctcggta ccagtgcgcc agaaaccatg accacaacat
    13801 cacctttctg agccagaccg ctctgcaaag ctagttcttt acccagacgg tagaagtcat
    13861 cagtagaggt gatttctttc accaactgcg gcacaacgcc tttgctcagc accagctgat
    13921 gtgcagtttt ttcgttggtg gtcagcgcca ggatggttgc atccgggaag tatttacgca
    13981 ccgcacgtgc agatttaccg ccctgggtcg caacaacgat cagcggagca tccagttttt
    14041 ctgcagtttc aactgcacca cggcaaactg cttcagtgat acgcagttta cggctgtcgt
    14101 tgttgaaatc cagacggctg gtcattacac ggtcagtacg ttcgcaaatg gtcgccataa
    14161 tagaaacagc ttccagcgga tacttacctt ttgcggattc accagacagc ataactgcgt
    14221 cagtaccatc cagaatggcg ttagcaacgt cacctgcttc tgcgcgggtt ggacgtgggt
    14281 ttttgatcat ggaatccagc atttgagtcg cagtaataac gactttacgt gcacggatac
    14341 atttttcgat catcatcttc tgagcgaaga taacttcttc aaccgggatt tcaacaccca
    14401 ggtcgccacg cgcaaccatg ataccgtcag acgcttcgag gatttcgtcg aagttgttga
    14461 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    14521 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    14581 ctacgccttg ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    14641 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    14701 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    14761 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    14821 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    14881 ctttcagaga aacgtcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    14941 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    15001 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    15061 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    15121 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    15181 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    15241 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    15301 cgtgagtgcc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    15361 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    15421 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    15481 aaggttacgc aaaaacttca ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    15541 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    15601 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    15661 atgctgatag ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg
    15721 aactgtaccg agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg
    15781 ggcaggaacc aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac
    15841 gcattcagcg cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa
    15901 aatgaccatt caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc
    15961 cagttaatca ttctaaataa caactgctga aattatacga atttcggtat gtattttaat
    16021 ttatagcgat aatgttattt ttattttctg caatgaacgt cacaaataat tgtcgcttaa
    16081 gttaaattta tttcaaatga taataaatat cagttcactt ataatttctt gctttgtaat
    16141 tttaattgat aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac
    16201 cataaaggat tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa
    16261 tatctggtgt tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct
    16321 gaattgtcag cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc
    16381 actattagat attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc
    16441 tggtctaaca attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga
    16501 tagcggcaca gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg
    16561 atgcacgggt tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg
    16621 gacattcttc tcccgtatca aaatccaccg caattatttc tttggcgaca acggggttgg
    16681 ctctggcggc ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa
    16741 agagccgcaa tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg
    16801 ctgtattacc gtcgatcata aacgctgtat tggctgtagc gcctgtacta cggcatgccc
    16861 gtggatgatg gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg
    16921 tgaatgcgca aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac
    16981 tgtgtgaatc ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga
    17041 gtcaatctca cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc
    17101 ggtggcatgg gcttcggcta caaaattatg tatgacgaag taccgccagg cacgaaacct
    17161 ttcgatgaag cgaataaatt agtctttgct accggcccat taactggatc tggtgccccc
    17221 tgtagttctc gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagttgtc
    17281 gatgcccata tgggtggctt ttttgcagcg caaatgaaat tcgctggata cgacgtcatt
    17341 attatcgaag ggaaggcgaa atcaccggta tggctgaaga ttaaagatga caaagttagc
    17401 ctggaaaaag ccgatttctt atggggaaaa gggacgcgcg caacgacgga agaaatttgt
    17461 cgattgacca gtccggaaac ctgtgtggcg gctattggtc aggctgggga aaaccttgtt
    17521 cctctctctg gcatgttgaa tagccgtaac cacagcggcg gtgcgggaac tggcgcaatt
    17581 atgggttcga aaaacctgaa agcgattgcg gttgaaggga cgaaaggggt caacattgcc
    17641 gatcgtcagg agatgaagcg tctcaacgat tacatgatga ctgaacttat tggtgcgaat
    17701 aacaaccatg tcgtgccaag tacgccgcaa tcgtgggcag agtattcaga tcccaagtca
    17761 cgttggacag cacgtaaagg gctgttttgg ggcgcggctg aaggtggtcc gattgaaacg
    17821 ggggaaattc cgccaggcaa tcagaacacg gtcggctttc gtacctataa atccgttttt
    17881 gacttaggtc cggcggcaga gaaatacaca gtaaaaatga gtggctgcca ctcttgcccg
    17941 atccgttgta tgacccaaat gaatattcct cgggtgaaag agtttggcgt gcccagcaca
    18001 ggtggtaaca cttgtgtagc caactttgtc cataccacca tcttcccgaa cgggccgaaa
    18061 gattttgaag ataaagacga tggtcgtgtg attggtaacc tggttggtct gaatttgttc
    18121 gatgactacg gcctatggtg taactacggg cagctgcatc gcgactttac ttattgttac
    18181 agcaaagggg tgttcaagcg tgttctgcca gctgaagagt atgcggaaat tcactgggat
    18241 caactggaag cgggtgacgt taatttcatt aaagattttt actaccgtct ggcgcatcgt
    18301 gtgggtgagc tgagtcacct ggctgatggt tcatatgcca tcgcagaacg ctggaatttg
    18361 ggtgaagagt actggggcta cgcaaaaaat aaactctggt cgccgtttgg ctatccggtt
    18421 caccatgcca atgaagcgtc agcgcaggtc ggttccattg ttaactgtat gttcaaccgt
    18481 gactgcatga cgcataccca tatcaatttt attggttcgg gtttgccatt gaaactgcag
    18541 cgtgaagtgg cgaaagagct ttttggttct gaagatgcct acgatgaaac caaaaactac
    18601 acgccaatca atgacgcaaa aattaaatat gcaaagtggt cgctgttgcg ggtctgtttg
    18661 cataacgccg tcaccctgtg caactgggtc tggccaatga ccgtttcgcc gctgaaaagc
    18721 cgtaattacc ggggcgatct ggcgcttgaa gccaaattct tcaaagcgat caccggcgaa
    18781 gagatgactc aggaaaaatt agatttagct gcagagcgta tctttacgtt gcatcgtgcc
    18841 tacacggtaa aactgatgca aaccaaagat atgcgtaatg aacacgatct tatctgttcc
    18901 tgggtattcg acaaggatcc gcagatcccg gtctttactg aaggtactga caaaatggat
    18961 cgtgacgata tgcatgcttc gctcacgatg ttctacaaag aaatgggctg ggacccacag
    19021 cttggttgtc cgacccacga aacattgcag cgtctggggc tggaagatat tgccgccgat
    19081 ctggcagcac acaatctact gcctgcgtaa ggaatgggtg agatgaacca tcgggatgaa
    19141 ttgccgttag cgaaagtcag cgaagttgac gaggcgaagc ggcaatggtt acaggggatg
    19201 cgtcaccccg ttgatacggt aaccgaacca gaaccggctg aaatcctggc agagttcatt
    19261 cgccagcatt ctgtggctgg gcagctggtg gcgcgcgccg tgtttctctc gccaccctat
    19321 tcggttgcag aagaggaact gtcagttttg ctggaaagca ttaagcagaa cggagactac
    19381 gcggatatcg cttgtatgac gggaagtcag gatgattact attactcaac gcaggccatg
    19441 agtgaaaact acgcggcgat gtctttgcaa gttgtggagc aggacatttg tcgcgccatt
    19501 gcacacgccg ttcgttttga gtgccagacg tatcctcgtc cttacaaagt cgccatgctg
    19561 atgcaagcgc catattactt ccaggaagcg cagattgaag ccgcgatcgc cgcgatggat
    19621 gttgcacctg aatatgccga tatacggcag gtggagtcat cgacggcggt gttgtacttg
    19681 tttagcgagc gatttatgac ctatggcaag gcttatggct tatgcgaatg gttcgaggta
    19741 gagcagtttc agaacccctg aaatttcagg gagatgtcat ggatagggtg gacagttgca
    19801 gccactgcat taggggataa tcagatgtcc ttcactcgac gcaaatttgt tctggggatg
    19861 ggaacagtaa tcttttttac tggttctgcg tcttctctgt tagcgaacac gaggcaagaa
    19921 aaggaagttc ggtatgccat gattcatgac gagtcacggt gcaatggctg taatatttgc
    19981 gcccgcgcat gccgtaaaac taaccacgtc cccgctcagg gaagccgttt atcgatagca
    20041 cacattccag tcaccgataa cgataacgaa acgcagtatc acttttttcg ccagtcatgt
    20101 cagcactgcg aagatgctcc gtgcatcgac gtatgcccga caggggcgtc gtggcgtgat
    20161 gagcagggga tcgtgcgggt agaaaaatcg cagtgcatcg gctgcagtta ttgcattggc
    20221 gcttgtccgt accaggttcg ctaccttaac cctgtcacta aagtggcaga taaatgtgat
    20281 ttctgcgctg agtcccgact ggcaaaaggc tttccgccca tttgcgtcag cgcctgcccg
    20341 gaacacgcat tgatctttgg tcgcgaagat agcccagaaa ttcaggcgtg gttgcagcaa
    20401 aataaatact atcaatatca gctacctggt gccgggaaac cgcatctgta tcgccggttc
    20461 ggtcaacatt tgattaaaaa ggaaaatgta tgaacccgtc gcaacatgct gaacagtttc
    20521 agagccagtt agcgaactat gtgccacagt tcactcccga attttggccg gtgtggttga
    20581 tcattgccgg agtattgctg gttgggatgt ggttggtgct ggggctgcat gccttgcttc
    20641 gtgctcgtgg cgtgaagaaa tcagtcaccg attatggtga gaagatttat ctttactgca
    20701 aagcggtcag attatggcac tggtcgaatg cgttactctt tgtattgttg ctggcaagtg
    20761 ggctgataaa tcactttgcg ctggtgggcg caactgcggt taaaagtctg gttgcggtgc
    20821 atgaagtttg cggatttttg ttactcgcat gctggctcgg ctttgtgctg atcaatgccg
    20881 ttgggggtaa tggtcaccac tatcgcattc gtcgtcaggg gtggctggag cgagcggcaa
    20941 aacaaacgcg attttatttg tttggcatta tgcaggggga agaacatcct ttcccggcaa
    21001 caacccagtc taaatttaat cccttacagc aggtcgccta tgttggtgtt atgtatggat
    21061 tgctgccgtt gttactattg acggggctgc tgtgtctcta tccgcaagcc gtgggagatg
    21121 tgtttcctgg cgtaagatac tggttattgc aggcgcattt tgctctggca tttataagcc
    21181 tcttttttat cttcggtcat ctttatcttt gcaccacggg gcgtacgcca cacgaaacct
    21241 ttaaaagcat ggtcgatggc tatcaccggc actaacgaat gatcattaca cgagcagacc
    21301 tgcgagaatg gcgtatcgga gcggtgatgt atcgctggtt cctgcgccat ttcccacgcg
    21361 gcggtagtta tgccgatatt catcatgcac tgattgagga aggatatacg gactgggcgg
    21421 agagtctggt tgagtatgcg tggaaaaaat ggctggcgga tgaaaacttc gcgcatcagg
    21481 aagtttcctc tatgcaaaaa ctggccacag accccggaga gataccattt tgcagtcagt
    21541 ttgcccgcag cgacgatcat gctcgcatcg gctgttgtga ggataacgcc cgaatcgcaa
    21601 ccgctgggta tgccgcgcaa attgccagta tgggttatag cgtgcggatt ggaagtgtcg
    21661 gctttaacag tcacattggc agctccggtg aacgcgcacg ggttgctgta accggtaatt
    21721 cttcgcggat aagcagcgct ggagatagta gtcggattgc gaataccgga atgcgagtac
    21781 gcgtttgtac attgggtgaa cggtgtcatg tcgccagtaa tggtgacctg gtgcaaatcg
    21841 ccagctttgg cgcaaacgcc agaattgcca acagcggcga caatgtacac atcattgcca
    21901 gcggtgaaaa ttcaacggta gtgagcacgg gcgtggtcga ctcaattatt ctcgggccag
    21961 ggggaagcgc cgcactggca tatcatgacg gcgaaagagt tcgcttcgcc gtcgccattg
    22021 aaggtgaaaa caatattcga accggagtcc gctatcgtct taatgaacag catcagtttg
    22081 ttgagtgctg atagctttac ctttaatcaa acgaaagacg ccgacgagca cgggatgcag
    22141 gctttacgac cgcccgagcc atcgcctcac caatttctgc acatgccgta agtccctgaa
    22201 cgaaaggctg gtcgtgcatc aaccagggca acgcgccgaa cgctacgcgt ccacgaatat
    22261 cttcgggttg ctgtaacgta taatcttcgc caacatcagg gatttcatcc cctgttttct
    22321 gtaattgctc gcgtagccca gggaaaggaa tatctttcac tttaagcgga cgctgtccgc
    22381 gggcatcaat aaaaacgtca aacgagtagc tgttgtcttc cgttttcagg acggtgcgcg
    22441 actcattaat ttccattttg tagtcttcac cgagggcgag aatatgaatg attcccgctt
    22501 cacgtaaggc aagtaggcga cgaatagact ctgaagggat tgccgcataa ttatcgatga
    22561 aaaccctggc aaggccttta ctgaaccgtt tatggtcgtg ttcattcaga tgaggaacaa
    22621 tttcctgtac ggcttcatgc aggcgcagaa tgacataacg ccagggaaca gtatgttttt
    22681 ctcgtttatt gcgttcgact tcctggagat ttttttctgc ccagtcaaat gggtcgcgtt
    22741 gtttgcgctc ggcaaaccag gcttgagcaa aggaatcgac attcaggctc tctaaggcta
    22801 tgcgttggct ccagtctgga tcagcaaact tgatttcctc tactatcaat ctaaataccc
    22861 gatccaaaag gccctcttca cctttttgaa tctcagcatt taatgcctga tcggtgacga
    22921 tgtgtaaggg ctcgtaggga atagggcaat agaaatcggc ttcgggtaaa atacccgtgc
    22981 gcgacatcaa cgtaatattt agcttttcac ttgcgttatc gcggttaaaa acgacgtgtt
    23041 gtttatcatc ttcaatgaac gaaccatgct gaatagccac tgccattgcc gcatccagtc
    23101 cgctcaagga tgttcccata atacccacgt tacacgcatc gacctttgct tccatcaggc
    23161 ctgaccacgg actgggaaaa tacgttctgg ttgcttcttc ttcatcaggc cagacgtgac
    23221 ccgtggcaat caccgctaaa tcaaacgtct cgctgggtaa atcctgattt gtagcgagca
    23281 tgacgccagc atttgtaatt tgcagatcgg taacctggca tgattcataa acagccactg
    23341 caaatttttg ctgtcgtgcc tggtctacta atcgtaaaaa ttgatcgcgg aaatattcgc
    23401 ccagcagaat tcgcggtaaa aactgacgat cgtgcaaggt ttctttttta acgccataac
    23461 gctggagatg gctggcttct tgcttttgta gccattcgag atacgtacaa ttaatcggcg
    23521 gtatttcaat actggcaata tttgccagca tcatttttga gttttcctca tcactgtatg
    23581 gcatcccgac accggcctcg tcagcctgct cgaagataga aatagaaagt ggagtttgtt
    23641 gctgtagaag cgagaataag gtgtagatcc ccgtaggccc ggcacccaca atagcaattt
    23701 ttttcattgt tacttacctg tacgcattct ttcgaattta gtgttatcag ataagtttgg
    23761 ttgagatttt tagaatttga gaaatttcag acagggatga aaagggcgct aaccgccctt
    23821 gattaaattc tgtcaggcga gttttgcctg attgatttga ctcagtggct cattgacatc
    23881 gaaaactttg gcgacaactt cctcaacgcc gagattttta agtcgggcag tatgtttttc
    23941 cagataggca agggcgcttt tttcatcagt aaaaagatag atcccaccgg cttcgtggtt
    24001 cttttcactt tctgtccata ccttccacag aaaaccaggt tcctgattaa tcgactcagc
    24061 aagcggttta agctgctcag ccattgcgtc gccaaatggg ccattaaaag caaaatgaag
    24121 ttgtaacaaa gttgccatag cgattctcct tctgataacg catacgaaga cagtgtaaat
    24181 cttttaacgt attggataca gagaagaaac agagggaaaa aggagaagac caaattaaga
    24241 aaatctggtg cgtccgagtg gactcgaacc accgaccccc accatgtcaa ggtggtgctc
    24301 taaccaactg agctacggac gcaggatggt gcgttcaatt ggactcgaac caacgacccc
    24361 caccatgtca aggtggtgct ctaaccaact gagctatgaa cgcaacgttg taggtgacaa
    24421 cggggacgaa tattagcggc agagtgggaa ggtggcaaga ggcaaaacgt aattttctgc
    24481 gctatttcga ccgtttgcag agcttttaag caaattggct atattttgtt gatttgcaag
    24541 ggcgattttt attcaggatc gcatttacat ctgatacaac ccaataattc gtgtatcaga
    24601 gtttaaacag agttgtttgc caccatctca aatctgtcgt ggataaatcc tccgtagcct
    24661 ttacacttag gtagcaataa aggcacacag atgcagcaaa ggaataaatc atgggatctg
    24721 atgcgaaaaa cttgatgagc gacgggaatg tgcaaattgt taagaccggc gaggtcattg
    24781 gcgcgacgca acttactgaa ggcgagttga ttgttgaagc tggcggaaga gccgaaaata
    24841 ccgtggtcac gggggctggc tggttgaaag tggcaaccgg tgggatcgcc aaatgcacac
    24901 agtacggtaa caatggcacg ctatcggtca gcgacggtgc cattgccaca gatattgttc
    24961 agtccgaggg aggcgcaatt agtctctcta cgctcgctac ggttaatggc cgccatcccg
    25021 aaggtgaatt cagcgttgat cagggttatg cctgcggttt gttgctggaa aatggcggta
    25081 acctgcgtgt actggaagga catcgcgcgg aaaaaatcat tctcgatcaa gagggcggcc
    25141 tgttggttaa tgggacaacc tcagcggtcg tggtagatga aggtggtgaa ttgttggtgt
    25201 atccaggtgg ggaagccagc aattgtgaga ttaatcaggg cggcgttttt atgctggccg
    25261 ggaaagccag tgatacgttg cttgctggtg gcaccatgaa taatctcggt ggtgaagact
    25321 ctgacactat tgttgagaat ggatccattt atcgtctggg gacggatggc cttcagctct
    25381 acagctccgg taagacgcaa aacctgtccg tgaatgtggg tggtcgggct gaagtgcatg
    25441 ccggtacgct ggaaaatgcg gtaatacaag gtggaacagt gatcctgttg tcacccacca
    25501 gcgcggacga aaattttgtc gtagaggaag atcgcgcacc ggttgaactg accgggagtg
    25561 ttgcattact ggacggcgct tcaatgatta ttggctatgg cgcagatctg caacaatcaa
    25621 cgattactgt acagcagggc ggtgtgttga ttctcgacgg cagtacggta aaaggtgacg
    25681 gtgtcacttt tattgttggt aacatcaatc tgaatggcgg aaaactgtgg ctgatcactg
    25741 gtgcggcaac gcatgtgcaa ctgaaagtga aacgcctgcg cggagaggga gcgatttgcc
    25801 tgcaaaccag tgcgaaagaa atctcacctg acttcatcaa tgtgaaaggg gaagttaccg
    25861 gggatataca cgttgagata acagatgcca gtcggcaaac tctgtgcaac gctctgaaat
    25921 tacagccaga cgaagacggg attggcgcaa cgctccagcc tgtgtaaaaa cacaggcgca
    25981 acccgtgaag gttgcgcctt gtctttatta gcgggatgct cgttgcagaa tgataactga
    26041 cggcagacgt tgcaggaacc gcatacgcag catcatcata atggctgccg acgtcaggcc
    26101 aataataaag cctatccaga agcctgctgg ccccataggt tcaacgacca gagcggtcag
    26161 tgccagaata tagccgcttg gcaagcccag cacccagtaa gccgtaaagg tgatatagaa
    26221 aatggaacgc gtatctttat aaccacgcaa aatcccactg ccaatcacct ggattgagtc
    26281 agaaatctga tataccgccg ccagcaacat caaatgcgca gccagcgtta caacctcggg
    26341 attgtcgttg tacaacaggg cgatttgctc ccgcagtgaa accgtgaata tggccgtcag
    26401 ggttgccata cagacaccca ccataagccc ggtccgcgca gcggtttgcg catccagcgt
    26461 tgagccctga cccagacgat aacctacgcg gatagttacc gctgccgcca gcgacattgg
    26521 aagcacaaac atcagtgaac taaagttcag ggcaatctgg tgtcctgcga catcaacaat
    26581 accgagcgga gacactaaca gagccacgac ggcaaacagt gtcacttcaa agaatagtgc
    26641 cagcgcaatc ggcaaaccga gttgaatcag tcgtttcata accgcaggat cgggtttcgc
    26701 ggtgcctttt tcgttacgaa tatcgcgcat ggagcgggcg cgtttaatgt aagaaaccat
    26761 ggcaaggaac atgacccaat acaccgccgc agtagccacg ccacaaccaa cgccaccgag
    26821 ctcaggcata ccgaaatgac cataaataaa gatatagttc accggaatgt tcaccagcag
    26881 gccgataaag cccattacca tacccggctt ggtttttgcc agaccttcac attggttacg
    26941 ggcaacctgg aagaacagat agcccggcgc gccccacaac aacgcacgca gataacccac
    27001 ggctttgtcc gccagagccg gatcgatgtt ttccatggag cggataatgt aacctgcatt
    27061 ccacagcaca agcataatga gaacggaaac aaaacctgcc agccagaaac cttgtcgcac
    27121 ctgatgcgca atgcgctcac gtcgaccgga accatttaat tgcgcgataa ccggcgttaa
    27181 tgccagcagc agtccgtgac caaagaggat cgccggaagc cagatagaag taccgatagc
    27241 gaccgccgcc atgtcggtgg cactatagcc gcccgccatc acggtatcga caaaacccat
    27301 cgcagtttgg gcgatttgcg cgagaatcac cgggattgct aatgctaata acagacgcgc
    27361 ttcactgata tacttctgca cgtgaacacc ttttatttgt agttatatga gagactaaaa
    27421 agccgcctga atgggcagca aaaagaagaa gcaggggaaa ttcagtctat tgtagcgagg
    27481 atttattaat tctccagtga aaaaatagtc attcttaccc cttcactggt aagggcgttt
    27541 gccacctgct attgtgctga acagaatgtc ttaactgatt tcaggagttg taagtatgtt
    27601 tacggggatt gtacagggca ccgcaaaact ggtgtcgatt gacgagaaac caaattttcg
    27661 tacgcatgtg gtggagttac ccgaccacat gctggacggc ctggaaacag gtgcttccgt
    27721 ggcgcataac ggttgctgcc tgaccgtgac ggaaattaac ggcaaccatg tcagttttga
    27781 cctgatgaaa gaaacgttac gcattaccaa tcttggcgat ttaaaagtgg gggattgggt
    27841 aaacgttgag cgtgcggcga aattcagtga tgaaattggc ggacacttaa tgtcaggtca
    27901 tattatgacc accgctgaag tggcgaaaat attaacctcg gaaaataatc gccagatctg
    27961 gtttaaagtc caggatagtc agttgatgaa atatatcctg tacaaaggat ttattggcat
    28021 cgacggtatt agcctgaccg tcggcgaagt cacgccaacg cgtttttgcg tccatttaat
    28081 tccggaaact ctggaacgca cgactcttgg gaagaaaaaa ctcggcgcac gcgtcaacat
    28141 tgaaattgat ccacaaactc aggcagtggt agatacggta gaacgtgtgc tggcggcacg
    28201 agaaaatgcc atgaatcaac caggtacaga agcctgatct gaaatcgccc ccggagaggg
    28261 ggcgatagaa tagcctttag cgagcgactc gaaggccgtt ttcaacaccg cgtgagaaca
    28321 cgacctgcca gagctgaata tcacgggcgc ggaaagcacc tgcacaggca ttcagataat
    28381 aggtaaacat tcgtttaaag cgttcactgt agttatccgc aatttctggc catgcggcga
    28441 ggaatcgttc ataccacgcc atcaacgtag tatcgtaatc agcaccgaag ttatgccagt
    28501 cttccatcac gaagtggggt tcgctggatt gagcaatctg gcgtacagag ggcaggcaac
    28561 cgttcggaaa aatatattta ttaatccagg gatcaacatt cagatcggtt tttttcgaac
    28621 cgatagtatg gagcaggaat ataccttccg gtttcaaatt acgatccacc accgcaaaat
    28681 aggtatcgta atttttcggt ccgacgtgct cgaacatccc cacagaaaca atacgatcaa
    28741 actggtcgtt caggtcacga taatcttgca gcaaaatggt gacatccagg ccttcacagc
    28801 gttcctgagc cattttttgc tgttcggcag aaatggtgac gccgaccacg cttacgtcat
    28861 agttagatgc catgtagtgc gccagtccgc cccagccgca gccaatatcc agtacgcgca
    28921 tccctggttt taactgcaat ttttcacaaa tcattttgag cttcgcctgc tgggcagatt
    28981 ccagattatc ggcatctttc cagtaagcgc aggaatattg catgaaggga tcaagcatgc
    29041 ggctgaacaa gtcattaccc aaatcgtaat gctccttgcc gactatccag gcacgttttt
    29101 tactctgcag attgaagaga cgagcgctgg caatacgcag cgtgtctttg aaatgatggg
    29161 ggagttggtt ctcgagacct gcgcgtaaga ctttgctaaa aaacatatcc agtcggtcac
    29221 attcccacca gccatccata taactttcgc ctaaccccaa agagccttct tgcagaacgc
    29281 gtttaaaaaa atcggggttt ttcacacgaa tatccgccgg ggcagaaccg ttaatggcta
    29341 taccggcacg gctaagtaat tcgttggcga tacggtacca gttgtcatcc ggtacactga
    29401 cttcttctat acacgatgaa ctcatagttt ctccatcact ggtcggtgat cagaacctta
    29461 aaacagcgta gacgcttttt gggctttgtg agaaatctcg cagaaaaaac cgcgagtcta
    29521 taatccgacg tagaacagag ggaaaggagg aacctttgcc gaaatgacca tccatagtga
    29581 gtcgggaacg atcctgtccc cgtaaaaaca taatatttat cgtagcttta acaactcgaa
    29641 attagtatag gccgctaatt tcaatcatac aacaaaattt tgttagcagc ctaagtataa
    29701 gtatatcgat atagatcagt gtgattcgct atgagcgact tcggcattgc catgattctg
    29761 gcagccaact tcttcacaac gttgcatcat gtaacccaga gcgaccagca ctactgttga
    29821 taacatcacg ctggtggtgg tgagcaatgg cgtgctgata ctgatcagcc aggaaactac
    29881 cagacttgcg aggaagcaca gacccagttg tagagtgttc tgcaacgctg cggcgcgacc
    29941 agttgcgtgt gggaagggac gcagcgcctg ggcgacaaca atcgggtaga tcgcgccatt
    30001 ggcaatcgcc atcacacaga atgggatcag gatttcgacc agcgacacat ggctaatgaa
    30061 gcctgcagcc caggtcgcaa tgacgctgac tgcaaacagc accagcaacc acggtaataa
    30121 ctgcttgcct tgccatttct gcagcgcggc gcgacagcca taaccaccaa tcagaaacgc
    30181 gatagtttgc gggacataac ttaaaccaat aactgccggg ctgtagccca tttcactaag
    30241 gatgaacggt gaaccggtca gccatgcaaa aaaactggct gaacaggccg cgtatatcag
    30301 cacgttgccg cgataggttt tagaacgtag caggtcggta aaggtcagac catcctgact
    30361 attgttacgg gccttcgtcg tgggtttgag ccagaaaata ggcagaatca gcaccacggt
    30421 aatggcaaac agggtggcga aaatcgcctg ccaggaaaaa tggaccagca gccagcttcc
    30481 taacagagga gccagggccg gagatagacc caccagcggc atgatggtcg caaaaattcg
    30541 gttaactttc tgtgaaggat aataatccgt cactaacgct tgccagataa ccgccgcggc
    30601 gcagacaccc acagcctgta caaaacgcaa taccagcagc gtagcggcgt tttctaccca
    30661 cagcatcccc agactaccta acgcaaaaat tgtcaggccg attaataaca ccggtttacg
    30721 accataacgg tcggagagtg gcccccacag aagctgggct gcggcaaaac cggcaaggaa
    30781 cagactaagg ctggcactga cagcagacgc aggcgtttgc aggtcggcct gtatggcggc
    30841 gaaagcaggc agatacatat cggttgccag aaaaccgagt acgctcaaac ccgccagcca
    30901 gactaaaaat cttttcccag gttgcatgta tattctctaa atctctcaat taaacaccgc
    30961 cgcaaagtgt agggagtgaa attagtgttg tgaaacggta atatttgatg actggtttca
    31021 aaaattttgc aggcagacta tgtggtcaga atattcactc gaagttgttg atgcggtagc
    31081 gcgtaatggt agttttagcg ctgcggcaca ggagctgcat cgcgttcctt ctgcggtcag
    31141 ctataccgtg cgtcagctgg aagagtggct ggcggtgccg ctctttgaac gacggcaccg
    31201 tgatgtggag ctgaccgctg ctggagcgtg gtttctcaaa gaagggcgct ctgttgtcaa
    31261 aaaaatgcag atcacccgcc agcaatgtca gcagatagcg aacggctggc gcggtcagtt
    31321 agctatcgca gtggataata ttgtcaggcc agaacgtaca cggcagatga tcgttgattt
    31381 ttatcgccat tttgatgatg tcgaacttct tgtctttcag gaagtgttca acggtgtctg
    31441 ggatgcgctt tccgacgggc gcgtggaact ggcgattggc gcaacacgcg cgattccggt
    31501 aggcggtcgt tatgccttcc gggatatggg gatgctaagc tggagttgcg ttgttgccag
    31561 ccaccacccg ctggcgttga tggatggccc attcagcgat gatacgttgc gcaactggcc
    31621 gtcgttggtg cgcgaagaca cctcgcgaac gctacctaaa cgtattacct ggttgctgga
    31681 taaccaaaaa agagttgtcg tgcctgactg ggaatcatcg gcaacctgta tctcggcagg
    31741 attatgcata gggatggtgc caacacattt cgccaaaccg tggcttaatg aggggaagtg
    31801 ggtagcactt gaattggaaa accccttccc ggattcggca tgttgtctga catggcagca
    31861 aaatgatatg tcgcctgcgt taacctggct gcttgagtat ctcggcgata gtgagacgct
    31921 gaataaggag tggctgcggg agccggaaga gactcccgca acgggtgatt aacgacgata
    31981 gtcgcggaac gggccgtcag ccacggagcg gcgttcaatc aagcgcggat gcacttcaat
    32041 ggactgcggt tcttcacgtt tgttgacgat acgatccaac agcatgttga acgctgtttc
    32101 acccagcgaa tcttttggct ggtggatcgt ggtcagcgcc ggcgtaaaat agcgggcgtt
    32161 gcgcacgtta tcataaccga tcagcgaaac atcctgcggg acgcgcagac ccatttcatc
    32221 agcagcacaa agtgcgccca ttgccatgat atcgccacca cagaagacgg cagtagggcg
    32281 atgcggctgc gacagtattt gctgcatggc gcgataacca gattcgggtt caaagtcacc
    32341 ctgaacaatc cagctttccg gcaccttgat catcgcttct tccatcgcct tcataaaacc
    32401 ggcaaggcgg cctgcgccgg tgttacgttc cagcgggccg gggatgacgc cgatttcgcg
    32461 gtgaccgcgt tcaatcagat aacgcccggc catgtagccg ccttcgaacg cgttatcaat
    32521 gaccgcatcg gtgaagtcag cttttgcttc accccagtcc atcaccacca ttgggatatg
    32581 gcgatactct tccagcatcg ccagcaacgg ctctgggtac tcagaacaca tcaccagcag
    32641 accatcgacg cgtttttgcg ccatcatcga cagataagcc cgctgtttct caagattgtt
    32701 ccacgcatta cccagaatca gggtgtaacc tttctggaag caattttttt caaccgcttc
    32761 aatgatctcg gcaaaatagg ccgcttcgct gctggtcgcc agcaaaccga tagacttggt
    32821 gtggttaacc ttcaggctac gcgccaccgc gctaggggag tagtgtaatt ctttaatcgc
    32881 tgcccacacg gcgttgcgcg tttcttcagc gacgaaacgt gttttgttga ttacgtgtga
    32941 cacagttgta gtggaaacgt ttgctcgttt cgctacatct tttattgttg ccattccatt
    33001 tcactccaga ccctaacttc agctcctgca aaatcgcaag gtaaacgttt gcctttacac
    33061 accttacgca caacaaaagt gttgcggtac gccggaaaaa cgacacggag cgtcaggaag
    33121 gggtcaatgg ccagtacgca aatagtgtaa gcgtggaatt ttggccgatc ttgacgaaaa
    33181 ggggaagtgg taaaaacact tatcattata aatgacggga gattttttca tcacagtgtg
    33241 taaaaatgcg taatatcact atttgtgtgg taataaaaaa ggagaaaact tgatgagcac
    33301 cgaccttaaa ttttcactgg taacaacgat tatcgtcctc ggtttgatcg tggccgtggg
    33361 tttgactgcc gcgctgcact gatttcctct cgagggagcg cgttctccct cgcatctcct
    33421 tcctgtttgt tagcaaaatg gcaaaagtgt tttctctttt tgttattttc atttttttgt
    33481 gattgctgtc atgctttcgc cactttaatg aatatcgcgt aatgacgcga agaggtggag
    33541 ataaggcatg aaaattaact atccgttgct ggcgctggcg ctggcgattg gcgcgtttgg
    33601 tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc gcggtgtgga
    33661 tgtctcgatt cccgctgccg gaatgttaat cagtgcctat gcagttggcg taatggttgg
    33721 cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt tgattttcct
    33781 gatggcaatt ttcacgctcg gcaacgtact ttccgccatc gcgccggatt atatgaccct
    33841 gatgctttca cgcattttga ccagcctgaa tcacggagca ttttttggtt tgggttcagt
    33901 cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca ctatgtttat
    33961 ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg gtgaaaccat
    34021 cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa tggtaagtct
    34081 gttcttctca ttacctaaag gtggtgcagg ggcacgacct gaagtgaaaa aagagctggc
    34141 ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg gagctggtgc
    34201 aatgtttact ctctacacct atatctctcc ggtactacaa agtattaccc acgcaacacc
    34261 agtgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttctctatcg gtaactatct
    34321 cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt tgttgctgct
    34381 gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg cagctattag
    34441 catggtggtg tggggcgcag caacctttgc ggtcgtaccg ccgttacaga tgcgcgtgat
    34501 gcgtgtcgcc agtgaagcgc cgggtctgtc ttcatcagtc aatattggtg cctttaatct
    34561 tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc tgggatacag
    34621 ctttgtgccg gtgatgggag cgattgtcgc gggactggca ttattgctgg tgtttatgtc
    34681 agccagaaaa caacctgaaa cagtttgcgt tgctaacagc taattaaaca aaaacgcaga
    34741 aggataacct tctgcgttac agttgctgac aacgtgcgat ttatcttgcc gaatgcgctg
    34801 ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat caggcaatgc tgcatttgcc
    34861 atcagttatt atgcagcgag atttttcgct acgaactccc agttcaccag cgcccagaag
    34921 tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg ttcccagaca
    34981 tcaacggtca gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt agaggttgaa
    35041 acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga accaaagttt
    35101 ttgatcgctg catcagtaaa ctgcgctttg aaatcggcaa agctgccaaa agatgcggcg
    35161 atagcttcag cgacttttcc agtcggttcg ccaccggcgt tcggtgccag gcagttccag
    35221 tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc agagctgcga
    35281 ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt gttcaggtta
    35341 gtgacataag tctgatgatg cttgccgtag tgatactcga tagtttccgc agaaatgtgc
    35401 ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc
    35461 ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata gggtagcaaa
    35521 ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg taaagtttta
    35581 gcgaagtgtt tttggggtca ttacccgacg agcgccaaca tagtggcgct gccagtagtc
    35641 ttcactgaga gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa taaatttgcc
    35701 gttgccgaca tacacgccga catgatcggc tgtgccgcgt ccctgagtac ggaaaaagac
    35761 caggtcgccg tttttcagtt cactacgttc gattggcgct gcatcacgca ggtgatacat
    35821 ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt aataaaccag
    35881 gccgctgcaa tcaaaaccgg tacgcggtga gctgccaccc caacgatatg gcttaccaat
    35941 ttgctgcatc agtttattca ttgccacttt cgtagctttt tgcaccttcg ctttgtgcgc
    36001 gtcggcaaga gttcctgacg cggcattttt gactttcaca caatgcgatt tatagccctt
    36061 acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg cgtgtttgct
    36121 gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacggaag aagcggtttt
    36181 gcttgtggtc gtacttttac tggcggcttt tttcgctgtt ttctgggtct ttttggtggt
    36241 tgccgtgctt ttctttttat ctgctttttt ggtgatatga gtggtagcag aactttccct
    36301 ggcttgcttt gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca
    36361 gagcgtgatc gaaatacggt ttatccgcgc cactgagcgt ccctctgtta aggcaggtct
    36421 atcatgcctg cgtctgattt acaaaacttg atgattctaa tgcatagaag aagagaaagt
    36481 taatagcttt tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa tgcagcagtg
    36541 aaagctaaaa atggttataa atacgccagt ttgccgcgca tcagagaaga a
//
LOCUS       DAHBHC010000050        36529 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHBHC010000050 DAHBHC010000000
VERSION     DAHBHC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.050"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..2093)
                     /locus_tag="M2A35_002921"
     CDS             complement(<1..2093)
                     /locus_tag="M2A35_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7199193.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTRKLT"
     gene            complement(2169..4310)
                     /locus_tag="M2A35_002922"
     CDS             complement(2169..4310)
                     /locus_tag="M2A35_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCB7199194.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(4350..4487)
                     /locus_tag="M2A35_002923"
     CDS             complement(4350..4487)
                     /locus_tag="M2A35_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199195.1"
                     /translation="MLFLYYAVERKVVSKGLRSREMAAAGNFGGSVPERKRNTVLSQP
                     L"
     gene            complement(4520..5038)
                     /locus_tag="M2A35_002924"
     CDS             complement(4520..5038)
                     /locus_tag="M2A35_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCB7199196.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            5735..6235
                     /gene="tssB"
                     /locus_tag="M2A35_002925"
     CDS             5735..6235
                     /gene="tssB"
                     /locus_tag="M2A35_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCB7199197.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            6270..6494
                     /locus_tag="M2A35_002926"
     CDS             6270..6494
                     /locus_tag="M2A35_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199198.1"
                     /translation="MTAHKNISETYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            6545..8020
                     /gene="tssC"
                     /locus_tag="M2A35_002927"
     CDS             6545..8020
                     /gene="tssC"
                     /locus_tag="M2A35_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HCB7199199.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPWSPADNPVKSFNYHEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            8027..8440
                     /gene="tssE"
                     /locus_tag="M2A35_002928"
     CDS             8027..8440
                     /gene="tssE"
                     /locus_tag="M2A35_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCB7199200.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSAYSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            8444..10294
                     /gene="tssF"
                     /locus_tag="M2A35_002929"
     CDS             8444..10294
                     /gene="tssF"
                     /locus_tag="M2A35_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCB7199201.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            10258..11340
                     /gene="tssG"
                     /locus_tag="M2A35_002930"
     CDS             10258..11340
                     /gene="tssG"
                     /locus_tag="M2A35_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCB7199202.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            11365..12645
                     /gene="tagH"
                     /locus_tag="M2A35_002931"
     CDS             11365..12645
                     /gene="tagH"
                     /locus_tag="M2A35_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCB7199203.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRMLREKQREPEA"
     gene            12642..13166
                     /gene="tssJ"
                     /locus_tag="M2A35_002932"
     CDS             12642..13166
                     /gene="tssJ"
                     /locus_tag="M2A35_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCB7199204.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            13169..14499
                     /gene="tssK"
                     /locus_tag="M2A35_002933"
                     /pseudo
     CDS             13169..14499
                     /gene="tssK"
                     /locus_tag="M2A35_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            14504..15265
                     /gene="icmH"
                     /locus_tag="M2A35_002934"
     CDS             14504..15265
                     /gene="icmH"
                     /locus_tag="M2A35_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCB7199205.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYIPCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            15274..18039
                     /gene="tssH"
                     /locus_tag="M2A35_002935"
     CDS             15274..18039
                     /gene="tssH"
                     /locus_tag="M2A35_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCB7199206.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLTMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            18036..18779
                     /gene="tagO"
                     /locus_tag="M2A35_002936"
     CDS             18036..18779
                     /gene="tagO"
                     /locus_tag="M2A35_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCB7199207.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            18784..20196
                     /gene="tssA"
                     /locus_tag="M2A35_002937"
     CDS             18784..20196
                     /gene="tssA"
                     /locus_tag="M2A35_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCB7199208.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            20305..23739
                     /gene="tssM"
                     /locus_tag="M2A35_002938"
     CDS             20305..23739
                     /gene="tssM"
                     /locus_tag="M2A35_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCB7199209.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGSRGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHTLGWLKEKRARQPLNGIILTLDLPDLLTADKRRREYLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRDWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGKDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWMPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            23750..25102
                     /locus_tag="M2A35_002939"
     CDS             23750..25102
                     /locus_tag="M2A35_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCB7199210.1"
                     /translation="MDSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            25126..25608
                     /locus_tag="M2A35_002940"
     CDS             25126..25608
                     /locus_tag="M2A35_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCB7199211.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDIYWEHTLAGTNAMSKWQDRVQ
                     "
     gene            25652..26565
                     /locus_tag="M2A35_002941"
                     /pseudo
     CDS             25652..26565
                     /locus_tag="M2A35_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26575..27054
                     /locus_tag="M2A35_002942"
     CDS             26575..27054
                     /locus_tag="M2A35_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199212.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(27191..27976)
                     /locus_tag="M2A35_002943"
     CDS             complement(27191..27976)
                     /locus_tag="M2A35_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199213.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(28308..28384)
                     /locus_tag="M2A35_002944"
     tRNA            complement(28308..28384)
                     /locus_tag="M2A35_002944"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28348..28350),aa:Asp,seq:gtc)
     gene            complement(28517..29248)
                     /gene="dnaQ"
                     /locus_tag="M2A35_002945"
     CDS             complement(28517..29248)
                     /gene="dnaQ"
                     /locus_tag="M2A35_002945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCB7199214.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            29313..29780
                     /gene="rnhA"
                     /locus_tag="M2A35_002946"
     CDS             29313..29780
                     /gene="rnhA"
                     /locus_tag="M2A35_002946"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCB7199215.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(29777..30499)
                     /locus_tag="M2A35_002947"
     CDS             complement(29777..30499)
                     /locus_tag="M2A35_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCB7199216.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            30533..31288
                     /gene="gloB"
                     /locus_tag="M2A35_002948"
     CDS             30533..31288
                     /gene="gloB"
                     /locus_tag="M2A35_002948"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCB7199217.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            31360..32718
                     /gene="mltD"
                     /locus_tag="M2A35_002949"
     CDS             31360..32718
                     /gene="mltD"
                     /locus_tag="M2A35_002949"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCB7199218.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(32766..33536)
                     /locus_tag="M2A35_002950"
     CDS             complement(32766..33536)
                     /locus_tag="M2A35_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCB7199219.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTLEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(33614..34414)
                     /locus_tag="M2A35_002951"
     CDS             complement(33614..34414)
                     /locus_tag="M2A35_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCB7199220.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            34655..35569
                     /locus_tag="M2A35_002952"
     CDS             34655..35569
                     /locus_tag="M2A35_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199221.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(35566..36369)
                     /gene="dkgB"
                     /locus_tag="M2A35_002953"
     CDS             complement(35566..36369)
                     /gene="dkgB"
                     /locus_tag="M2A35_002953"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCB7199222.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSQEGLAPEWD"
BASE COUNT         8862 a         9697 c         9449 g         8521 t
ORIGIN      
        1 gtgagttttc tggtcaggtc atagtggtaa cgttgcgtcc ggccgtcaaa accgcgctgt
       61 tcagtcagcc tgtcgaccgg gtcataccgg aacgtggact ggctgccgtt ctcgttggtc
      121 agcacagtga tgcgcccggc agcgtcatac cccatgctgc gcgtcagacc gccttccgtc
      181 acactcaccg gacgcccgcg tttatcatat tcgatggtgc tgcgtttacc gtccggtgat
      241 accgttgctg tcaggtcgcc tgcggcgctg tactcataac ggatttcacg cccctgcgca
      301 tccttctgac tgaccagttg cccacgcggg ttataactgc tgtaagtgct gatgccttcc
      361 tcccggtgaa cggcgatttg ttgaccgtac cggtcgtact cataccgcgt cgtgtacccc
      421 gagcagtccg tgaaggccag cagttgaccg taacggctcc atgccatctg ttttgtactg
      481 cccgtcgcgt cctgtatccc tgtcggcagc tcactcgccg ggtcatcata gctgtaccgc
      541 gttgtctcac cgctgcgcga ggtctccgcc gtcagccttc ccctctcatc atactcccgg
      601 ctgctgcgaa gcccgtccgg gtacgtcact gacgtcacct gccgctggct gttatagcca
      661 taccgcaccg tcctgccgtc cggccccgtc accgccgtca ccgcacccga cgccatatgc
      721 aggctgtact ccgtccgccg ccccgccgca tccgtctgtg ccttcagcct ccccgcctca
      781 tcatactcgc tgcgggtaat gctcccgtcc gcatgttcct tcttcaccac ccgcttcagg
      841 ccaccctcgc cttccgtgta cagcacctcc cgccggttca ggctgtccgt gatgattacc
      901 cggctctctc cgtactcaaa gcggtagtcc agcccctccg ggttgacctg ctccgtcacc
      961 cgaccggtat catcataccg gtagcagctc tccggcctcc ccgcataatg gtgcgccacc
     1021 atccgcccgg cgtgctccgc atcataagta aacccgcgca cctgcgttcc gctgcggtca
     1081 tacaccgccc gcagttcacc accggccgtg tacgtgtagc gcgccagcgg cgcagtcggc
     1141 tgttcatccg ggtatgccgg gtcgtgtgtc agccataccg cctccagccg gataccgtta
     1201 tccgtgccgt atcctgtacc ggcgggcagc gtgtcgggga aaaccagaga agaggaagca
     1261 gaacggggac cggcagggga agataaagac gtggcgcgct gtttacggaa tacttccgcc
     1321 cgctgtgcct gtgtggtcag caccaggtga aaacggcggc ccgcgccgtc cgtcaccccc
     1381 gtcaccgcgc ccgccacatc accctccgcc gcccggtgaa aggccagcgt ccgcccgaag
     1441 ccgtccacca cgcccgtcag cacccggtat gcgggcggct ccggcggcag cacctcgtcc
     1501 gcccccggca cccgctccgg ccagctgagt atccaccacg gcccctgaag gctgtttgtc
     1561 gccaggtaca catgcggact caggcgaaca tcttccggca gcacctgcca gagcgcgctt
     1621 agcggctgcg aactgtgctg cgccgctact ccgccccgcg ccagccagag tgactcgctg
     1681 cggctgtagc ttatctcgcc gggaaacagc ggctcaaagt gaatgctcct gccgccgctg
     1741 tcgttgagta tcaggccttc atcgcgtatc tgtaagcgga tatcgaaggg cgctttccag
     1801 ccgggaccaa acacccccac cggcgcgggt gttctggtcc ggtagctgct gtaggcgcgg
     1861 gaaagaataa acggcagcgg gccgggcagc gcaaggtccg tctcgcccgg cagcaccttt
     1921 gctcccagca gcgggttcac cgggttaccc ttctgctcct ctatcgggct gttgggacat
     1981 accgaacagg ccactcccgt cggcgcacct atcagcaccc ccgctgaacc ctgcacaata
     2041 tccagtccct tgcgggtcat atcaccctgc cgcgccgccg gttttccgcc catgaatcaa
     2101 ttccttctgc ttttaatatg gtttatctgg tcactgcacc agttccgttt taattttttg
     2161 cacctgactc aaaattgccg catatccggc attccggggg gtaaatcttt cggcacaggc
     2221 gcatccggta caggttgtgt ggcaccacag ttccagttaa tcactggcgc atcaccgttc
     2281 acatccgact ccccttccag atgaatgtgc gtaccattga gaaatatgct tccgtccttc
     2341 gtcagcacca gccttgcctt accacagcag agttcaagat gctcaccggc agaataaact
     2401 gctgtttgtc cggtgttttc cttcattgtc ttgcccaccg agaaggtgac gttattcccc
     2461 acattgacgc tgtagcccat tcccaccatc agtgatttat gcagccctac ctgtgaggac
     2521 tgcaacaacg ccaccgaggt attcataatg ccgcctaccg tcgtctggta agccacaccc
     2581 accgtcagcg cacgaatgat ccccaccttt tcaacctggt ttgatcccac tttgataatc
     2641 tggttactgc ccaccgtttc cacctggttg acaccaaccg tctgtatctg attaacgcca
     2701 atgttctgga tctggttatt ggtaacggtg atcgcctggt tgttacctat tttttcggca
     2761 tggttgttta tcacatcagt ggtgcggtta ttcagcacct cggtgttcat gttcttctgc
     2821 gcgtggatgt agacctgttc tttccctgtc gcatcgtcaa acttcagttc attaaacccg
     2881 ctgcccttat aggttttcga gcggatcgtc atctgcgtct tcgtccccgg caggctgccg
     2941 ggggtgcggt tttcctggtg gtaggtacgc cccataatga tcggctggtc cggatcgccg
     3001 ttgaggaagt ccacaatgac ctcctgaccc acacgcggta tcgccaggtt accaaagccg
     3061 gtgcctgccc acgcctgtgc cacacggatc cagcatgaac tttcctggtt tgacgggtta
     3121 tagcggtccc agttaaattt cacccgcacg cggccatgtt catcacagaa gatttcctcg
     3181 cctgccggtc ccgtcacgac ggcgctctgc gggccgtcca ccagcggttt cagcaacggc
     3241 tgtggccgcc atgtccggtc tgccgggatt accgcaaagt ggttatccag cgtggtgcct
     3301 gaacccctgc gtcccggtac cgcctgcggc tgttcgccgt gaagatcgct cgccaccacc
     3361 tgccattccc ggttcaggtt cgcctgcgga tgccccgtca gcacaattcg ccgtcccggc
     3421 catatctccg gcgaacggct tgttccgcgc gccacttctg cgttgttgcg ccagccatcc
     3481 atctgccagc gggcaaagtt ctgcccgtgg gcacccttga aacgtccggg gtagtcatac
     3541 acttcatact gtgtgcgctg gtaatcctgg tactggcctt cctgatcaaa acgccctgcc
     3601 cagccgggtc gtttaaaggt gtagtctttg gtcaccacgg aagaagggcg gatttgtgcg
     3661 ctgtagcgaa actggctgat gcagagggtg ctcacctcgg tacgggtgtt cggattccag
     3721 gggatctcaa aggactccgg cagataacgc acggtatcgc acaggaccag gctctggtcg
     3781 gtactttttt gcgcgtgctc ctcataaaag aagatgcctt cctccgccgc catccggcac
     3841 aggaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg atgtggctcg
     3901 ctgaacagcg ggctccactc ggtcaccccg ttttcctgca atatcgtgcc gaggatgctt
     3961 tcgatgtcct cattctggaa gatacggaag ttctggcgca gccctgtgcg ccacagcggt
     4021 gggcacacct tcatgctgta cagcatctgg tttttgtcgt tctcccccag ttcaaaccag
     4081 gtcaccatac ctttcacccg gcgctgtacg tcatcgccct gccatatcgt caggtaggcc
     4141 attttgtcga ggatctgctg gaattcaagg gagagaaact gctggctgac cagagagaga
     4201 tcgagggaaa aaagcgaaga gagtgactgg ttcagatgaa aggagaccac cgcaaaagca
     4261 tccggtggca ggccgtccac ttccagcgtg aaacgtaatc cggttgacat agcaacctcc
     4321 ggggttaagg aaaaacagac agttcgctgt taaagcggct gtgacagtac agtgttacgc
     4381 ttgcgttcag gcacgctacc gccgaagttt ccggcagccg ccatctcccg tgaacgtaac
     4441 cccttactga caacctttct ttctacggcg taatacaaaa acagcatccg gcctgatagc
     4501 aggatgctgt tggcttaact tatgcttcca gcggtgcgcg ccagtcgtcg gcaccggagg
     4561 tgccagattt gacgtgttcc cattcaattt tgcggtaggc cagtgaaact tcgagcaact
     4621 gggtgaactc gcgtttcgcc gggtcctggc aatgcggcat atgcagtttc atatcgacaa
     4681 tcgtcgaatc agtcaggcgg gtagtgaaat aatgctcctg tttgccttcc acagaggtac
     4741 gccaccagtg aagttcagtg gttggcagca tttcgccgga ggccagtgcg ttgtacatca
     4801 gcggaaccgc tttgttcagc gccacggtaa aaatgaaagg cttgtgagca cgctgacccg
     4861 caggctgacc ggactgagga tcggtcggga cagtaacgtt atgcaggaat tcctgcacca
     4921 gcatctcgtc ttcgtgcccc tgcacataga tgttgccgac agaatcagcg gtaaatgcac
     4981 ctgcagtaat atttccctgg gtctgaccag tgatggagat ataacacggt gttggcattt
     5041 gtaaactcct tgttaacgtg gttaattact acttacaatg acctgccgtt aatcccagac
     5101 aggcagatgc agctcacttc acggaaccgc atcatttttc agtatttttt acatacaaac
     5161 cgagctagaa tttacggttg tcgtaaaaac tatttttaaa atcattaaat ctcttattca
     5221 gcaataactt aataatatat atctggataa ccatacccaa ctgttcaaag tccgtattgc
     5281 ctccataaaa tggccaaagc tgaatcatga ataaatattg tatttatcct ttctgaatta
     5341 tcattttgat aatttttgag atattcctta aaaataccca cttaatcaat cacaaaacaa
     5401 atatataaat aagcaatcat tagattagct tatgtttaat gaaattatta tatacttcaa
     5461 taagttacat aaaaaatcta tatttaaatt attgccataa tacacctgat gcgaattgca
     5521 caggttcaca caatctattt aacgcttgca gaatacccct ctttacgatc gataaaaagt
     5581 ccttttctta tttattttga agcgtaaaat tcggggtgat gaaggagtgg tgattttatt
     5641 ttcaccgcca ttagggcggc attgccgata tttttttgcg gtacaaatat gccgtgttca
     5701 tggcctgtac gagataacat gacggatcac ttttatgagc aaaaaatttg aaggcagcgt
     5761 cgcgcctcgc gagcgtatta atatatcgta tgtcccaaaa acagatggcc agacggctga
     5821 agtggaactt ccgcttaaca tgctcgttgt cggcgatacc ggaaatacgc aggaaacatc
     5881 cccactggat gaacgtcagg cagtttccgt taacaagcac aatttcggtg cagtgatggc
     5941 tgaagcagcc attggcctca attttactgt ccccgccacg ctgaagggaa gcacaacaga
     6001 cgatgaactg aatgtggcgc tgaatatcaa atcactggat gatttttcac cagatagcgt
     6061 ggcccggcag gttccggaag tgaataaatt gctggaactg cgtgaagcac tgactgcact
     6121 gaaggggccg atgggtaacc ttccggcatt ccgtacccag ctccaggcat tgctggaaaa
     6181 tgaagagtcc cgcgagcagt tgctcaaaga aatcggtctg gtgagcaata aataaccacc
     6241 actatttcat taaacagggt tatccaggca tgacggcaca caaaaatatc tccgagacat
     6301 accacctctc tgttgcagac attctgcagg tggtttacca ggtatgtttc agcccgtccg
     6361 ttgaaatcaa ccaggatggt gtggctgcgc tgattacaac actggatcgc cgtatcagtg
     6421 atctattgga tgagattatc catttttgtg agtttcagca gagcgcgtcg cactggcagc
     6481 gcgtacttca ctgatggcga aaagccggtt tcagaaacac agctaaaaca gaaggatttt
     6541 cagtatgtct ttacaggaag aggaactcgt ttccagtcat gccgggcagc ctgagcaggc
     6601 atcgtcgctg cttgatcaaa tcatggcaca gacccgtatt cagcccggtt cggaaggtta
     6661 tgacgttgcc cgccagggtg tgacagcctt tattgccagc atcctgcaat ccactgcatc
     6721 tgccgaaccg gtcaataaac tggccgtgga cagcatgatt gcagatattg atgaacgcat
     6781 cagccgccag atggatgtca tcatccatgc gcctgccttt cagcaggtcg aatcgttctg
     6841 gcgctcactg aaaaccatgg tggatcgcgt tgatttccgt gaaaacatca aggtcaacgt
     6901 gcttcatgtc accaagcagg aacttctgga agactttgag tttgcgccag aaattattca
     6961 gtccggattt tataagcacg tttattcgtc cggattcgga cagtttggtg gtgaacccat
     7021 cgctgccgtg ctgggcgctt atgagttcaa aaataccgca cctgacatga aactgctgca
     7081 atacgtcagt gccgtgggcg caatggcgca cgcaccgttc ctgtcctccg tttccccgga
     7141 gtttatgggg ctgaactcgt ggaccgaact gcctaatatc aaagatcttt atgccatctt
     7201 tgaaggtccg gcctacacca aatggcgcgc cctgcgtgac tcggaagatt cgcgctattt
     7261 agggcttact gcaccccgct tcctgcttcg ccagccctgg tcaccagcag ataaccctgt
     7321 taaaagcttt aactatcacg aagatgtcag ccagaaccac gaagattatc tgtggggtaa
     7381 tacggcctgg atgctggcat gcaatatcgc agacagtttt gccaaatacc gctggtgtcc
     7441 aaacattatc ggcccgcaaa gcggcggcgc ggtgaaagat ctgccggtgc atctgttcga
     7501 aacgatgggg cagattcagg ccaaaatccc aaccgaggtg ctggtcaccg atcgccgcga
     7561 atttgaactg gctgaagagg gttttatcac cctgaccatg cgtaaagact ctgataatgc
     7621 agcctttttc tctgcaaact cggtacaaaa accgaagcac tttccgggaa aagatgcaga
     7681 aaccaactat aagctgggta cgcagcttcc ttatctcttc atcatcaacc ggttagcgca
     7741 ctacatcaag gtgttgcagc gtgaacaact ggggtcatgg aaagaaagga gcgacttaga
     7801 gagggagctt aatacctgga tccgtcagta tgttgccgac caggaaaacc cgcctgcgga
     7861 cgtgcgcagc cgtaaacctc tgcgcgcagc cagagttgag gttatggatg tggaaggcga
     7921 acccggctgg taccaggttg cgctaagcgt gaggcctcat tttaaattta tgggggcaaa
     7981 ttttgagctt tccctggttg gccggttaga cagggagtaa cacaccatga taccggatcg
     8041 ggaaagaacc acaggttctc tttttgagcg tatggaagcc tcttcggcca gaaaccggca
     8101 gggaggcagt attcattcgc tcaggcaatc cattcgccag aacctacgaa acattcttaa
     8161 tacccggtcc ggtagttgcc ggggtgcgcc tgagctgggc attgatgaac cggaaggggc
     8221 tgaaaacttc cgcgaatcca tgagtcgggc catcgagcaa tgtatagaac gctatgaacc
     8281 ccggatctca cacgctgaag tgcaggcagt ggtttcatcc gcctacagcc cgctggatat
     8341 gacgtttcat attacagcct gggtgacatt taacgaaacg catgaggtgc ttgaatttga
     8401 tatggcaccg aatggcagcc agcattaccg ggtggactga caaatggaat ttgaagaacg
     8461 ctatttccgg gaagaactcg attacctgcg ccagcttagc aagctgctgg caacggaaaa
     8521 accccatctg gcccgcttcc tggccgaaaa agatgcggat ccggatattg aacgcctgct
     8581 ggaaggggtg gcttttctta ccggcaatct ccgccagaaa attgaggatg aattcccaga
     8641 actgacgcac gggcttatta agatgctatg gcctaattac ctgcgtccgg ttccggcaat
     8701 gacccttatt gaatatacgc cggatatgga taagtcttct gtaccggtgt taatcccccg
     8761 taatgagcag tttacaacca acgccgggga aatcagagtt gatgaagtgc tgccctctga
     8821 tgctaaaaag gaggagccgc ctccctgtac cttcactctc tgccgggata tctggctgct
     8881 gcccgttcgc ctggagcaga ttgaaaaccg cagtacgacc cgtaatggtg ttatcaacat
     8941 caccttttcg gtcgcaccgg gaacggactt ccgcacgctg gatctgaaca aacttcgctt
     9001 ctggctcggc aatgacgaca actatacccg tgaccagctt tatttatggt tctgcgaata
     9061 cttgcagggt gccgacctga ctgtgggtga acagcatatt cgcctgcctg agtttatgct
     9121 aaaagctgtc ggttttgagc cgcaggatgc catgctgccc tggccgaaaa acgtccacag
     9181 cggctaccgg atccttcagg agtatttctg ttaccccgat gcgtttctct tttttgatct
     9241 ttgtggttgt ccggctttgc ctgacggatt gcaggcggaa ttctttaccc tgcaactgcg
     9301 tttttcgcgc cctttgcccg tggacatccg gctgcgccgc gattccctgc gcctgtattg
     9361 cgcacctgcc attaatttat ttatccacca tgcagaagcc atcacgctgg acaaccggcg
     9421 ggcagactat ccgctggttc ccagccgcca ttacccacaa cattacgatg tattttccgt
     9481 taacagtgtg gtgagccagg tccaggatat gttcaggaaa aaagatctgg ggcgtcctgt
     9541 ttcgacgcag gccgcgcgcc agtggccagc ctttgaaagt ttcagccatc agatggaata
     9601 cagccggaag cgggaagtgg tgtactggca tcaccggacc aaaacatccc tgttccatcg
     9661 cggctttgat catacccttg cctttataca tgctgatggc agttatccgt cagacgaatc
     9721 tctgctcagt aatgaagtgg tttcggtatc gctgacctgt accaaccgtg agcttccgtc
     9781 acaaatacgt tccggcgata tcaccggcac aaccggtaaa aatgcggctg ttgcttcatt
     9841 tcgcaacatt acccgcccga cgcaaccact ctggccggtc attgatggca gcctgcactg
     9901 gtccctactc tccgccatga acctgaatta tctgtcatta ctggatacgg acgcgctgaa
     9961 gcaggtcatc gccaactttg atcgccacgc aatccatcat ccgcagacgg cacggctgtc
    10021 acaacaaaag ctggatgcca ttgagcgtct ggagacccgc cccgttgatc gcctgtttac
    10081 gggtattccc gtccggggac tggcctccac gctgtatctg cacccggagc cgtttgtctg
    10141 tgaaggggaa atgtatctgc tcggtacggt gctttcgcat tttctgtcgc tgtacgccag
    10201 cgttaactca ttccacatgc tgaccgttgt gaacacagaa agccaggaga catggaaatg
    10261 gacggaaaga atcgggcagc atcctcttat ctgagtccgg gaaatccccc ggcagataaa
    10321 gagcagaatg atccgctggc acaggttttc cataatgcct gctcttacaa tttttttgcg
    10381 atggcggagc tgttgcaccg cctggcaaag ggtgaaaagg ggacgccaga attatccctg
    10441 cgtgacgatc cggcacagga aaccctgcgt ttcagcgccg atgccagcct tgccttcccg
    10501 tgcagtgata tcagtgcact gaaacgggat acgtcagggg cattcaggat gaccaccacc
    10561 tttatggggt tacaggggag ccagtcgcct ctccccggtt attacctcga tcacctggcc
    10621 tggaaagcgg tacatgaaca aagtccggta ggtgattttc tggatatgtt cagccaccgc
    10681 ctgacccagt ttgtctggca tatctggcgt aagtaccgct atcacatcag ttttcgtaac
    10741 ggcggtgtgg atgcgttctc gcagcgcatg tactcccttg tcgggctggg ccaccgccag
    10801 cttcgcgata agctggcgat taatcacagc aaaatgctgg cgtattccgg gatcctggca
    10861 aatccgggac gatccccgga aataatctgt ggcctggtgt cccactgctt tgatttgtca
    10921 gaagtgacgc tgcaaaactg gcaacgtcgc aaggtggata ttgaaccgga tcagcaaaat
    10981 agtctgggga gctattctct taaaaatggc gaaaaactgg cgggaagatc ggtactgggc
    11041 aactttgttc tgggtacacg agtgcccgat ctcagcggaa aattccagct cagtattacc
    11101 agcctgaccc gaaaacagtt cctctctttt ctgccgtccg gcgaaaactt cctgccactg
    11161 acgatgttcg tgtccttcat cctgcgcgat cagcttgcct gggacttaca tctggggctg
    11221 gcgccggagc aggtgggcgc aatgcgcctg ggcgataaca aaagtgcgct gctgggctgg
    11281 accagcttcc tcggcacacc ggaagaacga ccctcagtca ccatcagggt acggtcgtaa
    11341 attatgataa tcacggagca gaatatgccg gaagagaaac tacagacgct ttcattgcag
    11401 gtcattaacg gcagtgagct ggaaagcgga cgggcggcgc gctgtctgtt cacacagcag
    11461 ggaaatgtcg gccatggccc cgaatgccac tggtcggtac aggatcgtca gcagagcatt
    11521 ccggcccagg cttttaccgt tatcctgcac gatggcacat tttgtctgcg cccgcagacg
    11581 gcacaactgt ggctgaatca ggcaaaagtc acagcaacat cagacctaat acagttgcgc
    11641 cagggcgatg agatccagat cggacggctg atggtgaggg ttcatctgaa ccggggagat
    11701 attccccatt acgatgagga aatggccact cccgaaacca tcgttaccaa tcgcgatatg
    11761 ctcacggata ccctgctatc aacggagggt acgccacact atccgggaat gactcaccgg
    11821 caccaacttg cagacaccgt ggtaaacggt ttttctgccg atccactcca ggcacttcag
    11881 tccgaaagcc tgattaccac gggcgatccg ctttcaggca ttgcggctgt ccggccatcg
    11941 gcaccgctgt ccgatccggc aagtaatggg gggatcaata ctccgtttat ggatctgccg
    12001 cccatttatg ccagccccgg cgatcgtaat gacgacgtct ctgcggcaga aatggcgcaa
    12061 cgccaccttg cggtcacccc cttactgcgc ggtcttggcg gctcgcttac catgagcaat
    12121 tccgacgatg cggatgattt tctggaggag gccggacgaa cgttacaggc cgcaataaaa
    12181 ggtctgctcg atttgcagca gcagcgtaac agcctctcag acaaacattt gcgcccgctg
    12241 gaagataacc cgctgcgcct gaacatggat tacgccaccg cgctcgacgt aatgtttgcc
    12301 gaaggtaaaa gcccggtaca tctggcggct cccgccgccg tcagtgaaag cctgcgcaat
    12361 gtccgccacc acgaagaagc taaccgggca gcgattgtgg agtcgcttcg tgtcctgctg
    12421 gatgctttct caccacaaaa tctgctgcgc cgctttgtgc agtaccgccg cagccatgaa
    12481 ctgcgccagc cgctggatga tgccggagca tggcaaatgt acagccatta ttacgaagaa
    12541 ctggcctccg atcgccagca ggggtttgag atgctgttta acgaggtcta cgcccaggtc
    12601 tatgaccgga tgcttcgtga aaaacagcgg gagccggaag catgaacagg aaggcgtttc
    12661 tggcctgcgt gctgatgtgc gtcatattaa ccggctgcga aacggcgaaa aaaatcagtg
    12721 aggtgatcaa aaacccggat atccaggtcg ggagcctgaa ggagcaacct tcggaaataa
    12781 ccgtcaccct gctgaccgaa ccggacacca ataccaatgc ggaaggtgaa tcagccgccg
    12841 tggatgtgca actggtgtac ctgactgacg actcaaaatt gctggcagcc gactatgacc
    12901 agatagccag tacgccgcta ccggatgtgc tgggtaaaaa ctatatcgac caccaggatt
    12961 tcaacctgtt gccggacacc atcaaaaccc tgccgccggt aaagctggat gagaaaacgc
    13021 agtttattgg tgtcgtggcc tatttctccg atgaccaggc cactgaatgg aaacaaatcg
    13081 agacggtgga aggaaccggc caccattacc gcctgctggt gcatgtacgt caaagcagca
    13141 tcgaaatgaa aaaagaggat gaataattat ggcaaccacc cgcaacaagg tgatgtggca
    13201 ggaagggatg ctgatgcgcc cacaccattt ccagcaacag cagcgttaca acgactacct
    13261 ggataaccag cgtttccggg ccatgaatga tttatcctgg ggatttaccg aactcaccct
    13321 caacaatgaa ctgctggcgc agggtaagat catgattgac agcgcgtcag gcacactgcc
    13381 cgacggcacc gtcttttcta tccccgacca ggacgcactg cccgatccgc tgcacccgca
    13441 aaattttccg gacgaaagaa gccgcaatat ctacctcgct ctgccggtcg ccagtgatgt
    13501 gagaaatgaa atcagcgacg gacggcgaat cgggcgttac cggctgaatt atgccgatgt
    13561 ccgggatttg cattcagaag aaggtgacgc gcgaacattg acgctgggtc aactgacgcc
    13621 gcgcattatg agcggtgcag aagatatgag cgcctatatt acgctcccgc tttgccgtat
    13681 cagcgatcgc catgccgatg gctccctgac gttggatgac gattttatcc cctcctgcca
    13741 gaatattcag gtcagtaaga aactgcgtgt ttatctcaaa gaggtacagg gggccattgg
    13801 cggacgggca agcgatctgg caaaccgtat tggctctccg gcgcagagcg gcatcgcgga
    13861 tgtggcggaa tttatgatgt tgcagttact taaccgtaac cagacccggt ttacccatcg
    13921 cgcccgccga tcccagctcc acccggaaga tttctacctt gatcttgctg ggttgctggg
    13981 tgaactgatg acctttaccg agccgtcgcg cctgccctgc ccgcttgatg tgtatgatca
    14041 tcatgatctg accaaaacat ttaaaacact gttaccggaa gtcaaacggg cgctacatac
    14101 cgtactgtcg ccaagagcgg tcaatctgcc gctgcatctg cgtgacggta tctggcaggc
    14161 cgatatccat gacacagaac tgctgcaatc tgccaccttt gtgctggccg tggcggcaaa
    14221 cgtgccggtc gatcagatcc agcgtcagtt tatccagcag tcgaaaattt cctcgccgga
    14281 aaaatccgca atatggtcag tgtgcagatc cccggtattc cattgcgtgc ccttatggtg
    14341 gctccccgcc agcttcctta tcattccggg ttcagctatt tcgaactcga caagagcgga
    14401 caggcctgga cagaaatggc tgccgccggg gccgttgcac tgcatgtatc cggcagtttc
    14461 ccggatctga acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact
    14521 gtctctgcca ccatttaccg gatacgacga aaaaagccag cgcaactacc atctcgcgct
    14581 tcgtggcaac agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat
    14641 gcgcctttca accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac
    14701 ggatgttcag gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat
    14761 ctcgtttcgc tacatcccct gcacgtttat tgatgaagcg gcgctcggta acggctggtc
    14821 aaacaagaac gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg
    14881 tggagagaaa gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga
    14941 tctgctggaa tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc
    15001 agcacaggat cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa
    15061 actgcgcggc gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg
    15121 ttatcagctc atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct
    15181 ggcgttgttt tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca
    15241 ccagctaaac aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg
    15301 gtaaaacggc tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg
    15361 agtcagcagg cagcggaaat taccgtcagc catgtgctca ttcagatgct caccatgcca
    15421 cgcagtgacc tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag
    15481 gcgctgacgg tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc
    15541 ccgatgctgg ttgagtggct gaaagagggc tggctgctgg cgtcggctga gatgcagcac
    15601 agcgaactgc gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata
    15661 ccgcctgctg ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt
    15721 gtgcagtgga cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca
    15781 ggcacaatga cggacgcctc tgacaccctg cttgcccgct atgccaaaaa catgaccgca
    15841 gacgcccgta acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg
    15901 atcgacattc tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcgggcgtg
    15961 ggcaaaagcg cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac
    16021 aagctgaaaa acaccgatat catgaccctt gacctgggcg cattgcaggc cggggcgtcg
    16081 gtgaagggtg aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg
    16141 gtgccggtca ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag
    16201 ggcgggctgg atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc
    16261 atcgccgcca ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg
    16321 cgccgcttcc agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg
    16381 cgcggtctgt cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg
    16441 caggccgctg cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg
    16501 attgatgtgc tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag
    16561 caaatctcgg cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag
    16621 cgcgagcttc gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag
    16681 tatgatgaaa cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg
    16741 ctggtccggg agattattgc gctgcgccag cagttactgg gcgtggcaga ggacgatgcg
    16801 gcgccgttgc cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagaacag
    16861 gataataccg gtgccgtacc ggctgatgag gccgacagag aacagccgga agagaccgct
    16921 gaaacagttt ccccggtaca acgtctggca catctcactg ccgaactgga cgccctgcat
    16981 aacgaccggt tgctggtttc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc
    17041 gaatggaccg gcgtgccgct taaccgcctg tcgcagaatg aaatgtcggt catcaccgac
    17101 ctgccaaaat ggctcggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcac
    17161 aaacacctgc tgaccgcacg cgccgacctg cgccgtccgg gacgcccgct gggcgcgttc
    17221 ctgctggctg gccccagcgg cgtgggtaaa actgaaaccg tcctgcaact ggcagagctg
    17281 ctctacggcg gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacat
    17341 accgtctcgc gactgattgg ttcgcctccg ggctacgttg gctacggtga aggcggcgta
    17401 ctgaccgaag cgattcgcca gaaaccctac tcggtagtac tgctcgatga agtggaaaaa
    17461 gcgcacccgg atgtgctcaa cctgttctac caggcgttcg ataagggcga aatggcagac
    17521 ggtgaaggcc gcctgattga ctgcaaaaat atcgtcttct tcctgacgtc caacctcggc
    17581 taccaggtaa tagtcgagca tgccgatgac ccggaaacca tgcaggaagt actgtatccg
    17641 gtgctggccg acttcttcaa acctgccctg ctggcgcgta tggaagtggt gccgtacctg
    17701 ccgctgtcga aagagacgct cgccaccatc attgccggga aactggcccg cctggataac
    17761 gtgctgcgca gccgctttgg cgcggaggtg attatagaac cggaagtgac ggacgaaatc
    17821 atgagccgcg tcacccgcgc ggaaaacggc gcgaggatgc tggaatcggt catcgacggc
    17881 aatatgctgc cgccgctctc cctgctgctg ttgcagaaaa tggcggcgaa tacggcggtt
    17941 gcccggattc ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag
    18001 aacgacgagt ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc
    18061 agacaggatg gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag
    18121 aagcatcgtc aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga
    18181 cagccacgtt acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg
    18241 actgctatga ccgcctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca
    18301 aagccggttt tgtgggcgaa gcctggaccc gcgccacgga acaggaaaaa caccgccagg
    18361 gcaacaccac agagctgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa
    18421 ccaccccggc tatcggtcat gttccgccac gcccggtgct gatgttcagt tgcgtggaca
    18481 atatcacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca
    18541 ctctgaatgc cgacaaccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc
    18601 tgctggagtc cagccggggg ttatccggga ttgatgaaat taagcaactg ttcggcgcaa
    18661 aaacgctgac ggtggatacc ggcgcagaca atgccgccgg aaagctgacc tttaacattg
    18721 acgggctggc aagggcgatt gctcccctgc gcgatgcctg ccactgggcg ggagaataaa
    18781 aatatggcaa tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg
    18841 ccggaagaga aactggcatc ggcgctgaaa gatgacaacc cgaactggga gtatatcgac
    18901 ggcgaaatcg tcaagctggg atcgctggcc cacagtcaac tggatatccc ggaactccag
    18961 cgccgggggc tggtcattct ggcttcggag agtaaggatt ttcgtctgct ggcgcatctg
    19021 ctgagaaccc tgcaacacgc cggtgatcct catctcgcat tgcgcctgct ggcgctgtat
    19081 gtggagcatt actgggcggt ggctgcgccg cagaatatgg cgcataaaaa acgctttgcc
    19141 agccaggtca ttaaacgctt tgaaacaggt attgaagttt tttcacagaa cgccgccaca
    19201 gcgcagcgcg atgcgctgtc aggggaactg gcaaaactgg cgcaatgctg gcagtcacat
    19261 aacgccccgg aactggcaca ggctactgat gatctttttg ccctgtacca gcgtgcgttt
    19321 aatcgtgcgg caccggctcc tgtccccact ccggcggcct ccggtagttc accacagacc
    19381 accgccacgt ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca aatcgtcatc
    19441 gacagtcacg acgacaaagc ctggcgcgac acgctgttaa aagtggcggc cattttatgt
    19501 gaacgccagc cggattcacc gcagggctac cgtctgcgcc gccatgcgct ttggcagaac
    19561 atcaccagta cgccccaggc ggaaagcgat ggccgtactc cactggctgc ggtctccgcc
    19621 gatatggtgg cagattacca tgctcagctc ggtagtgccg atatggcgct gtggcagcag
    19681 gtggagaaaa gcgtactgct ggctccttac tggctggatg gtcactgcct ttctgcacag
    19741 acggcgctgc gtctgggtta caaacaggtg gcagacgcca tccgcgatga ggtcatccgc
    19801 ttccttgagc gtctgcccca gcttaccggg ctgctgttta atgaccacac accgtttatc
    19861 agtgagcaga cgaaacaatg gctggcagct tcgcccgacg ccaaagttgc accggttgcg
    19921 caaatcggtg aggaatcgaa ggcagccaga gcctgttttg ctgagcaggg tctggaggcg
    19981 gcgctgcgat atctggacat gctacccgaa ggcgatcccc gcgatcagtt tcaccgccag
    20041 taccttgccg cacagttgac ggaagaggcg gggctggtac agcttgcgca gcagcagtac
    20101 cggatgttgt tcaggatggg attgcagatg atggtggcag actgggagcc gtccctgctt
    20161 gagcagctag aacaaaaatt cacggcagaa caataaaaga gaacaggagt ttttgtgttc
    20221 agattaccaa caccccgact actcagcgga ctcaaatcag ccctgcgacc ggcgatgccc
    20281 cggtttaaag tctctgcttt ctggctgctg atactggcgt ggatttttct gctggtgtgg
    20341 atctggtgga aaggcccgac gtggacgctg tatgaagaac agtggctcaa accactggcg
    20401 aaccgctggc tggcaacggc ggcgtggggg attattgccc tgatgtggct taccgtccgg
    20461 gtgatgaagc gcctgcaaca gcttgaaaaa cagcaaaaac aacagcgcga ggaagccatt
    20521 gatccgctca gtgtggaact gaacgcccag cagcgttacc tcgaccgctg gctgctgcgc
    20581 ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcaat tgccgtggta tatggtcatc
    20641 ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg ggtttccgtc agacattatt
    20701 tatgccccgg agggctcgcg cggcgcagaa caacgcctgt accttacgcc ccatgtcggt
    20761 aaacaggcgg tgatctttga tatcgacggc acactctgcg ctcccgctga tgcggatatc
    20821 ctgcatcgcc gtctgtggga acataccctc ggctggctga aggaaaagcg cgcgcgccag
    20881 ccgctgaacg ggattattct gacactcgat ttacccgatc tgctgaccgc agacaagcgc
    20941 cgccgtgagt atctgctaca gacgctgcgc agccgcttgc aggatatacg ccagcatctt
    21001 cactgccagc taccggttta cgtggtactg acccggcttg atttattgca gggttttgcc
    21061 gccctgttcc agtccctgaa cagacaggat cgtgatgcga ttctgggtgt cacgttcacc
    21121 cgccgtgccc atgaaaatga tgactggcga acagagttga atgctttctg gcagacatgg
    21181 gtggatcgaa tgaatctggc gttgccggat ctgatggtcg ctcagactca cacccgcgcg
    21241 tctttattca gtttttcccg ccagatgcag ggaagccgtg aacccctggt gtcactgctt
    21301 gaaggtctgc ttgacggcga aaatatgaac gtgatgctgc gtggtgtcta tctcacctcg
    21361 tcactgcaac gcggccagat ggatgacatc tttacccagt ccgccgcccg ccagtaccgg
    21421 ctgggcaata acccactggc gtcctggccc ctggtggaca ccgcgcctta tttcacccgc
    21481 agcctgttcc cgcaggcatt actcgcagag cctaatctgg caacggagag ccgtgactgg
    21541 ctgatgcgtt cccgtcgccg cctgacggtt ttctctgcca caggcggcgt ggcggcactg
    21601 ctgctcatca ccggctggca tcactattac aacggtaact atcagtccgg catcaccgtg
    21661 cttaagcagg ccaaagcctt tatggacgtg ccgcctccgc aggggaaaga cgactacggc
    21721 aacctgcaac tgccgctgct gaacccggta cgcgatgcca cactggccta tggcgactgg
    21781 ggcgaccgca gccgtctggc cgatatggga ctgtaccagg gacgacgtat cgggccttat
    21841 gtggaacaga cctatctgca actactagag cagcgttacc tgccctcgct gtttaacggg
    21901 ctggtcaaag cgatgaacgc cgcgccgccg gagagtgagg aaaaactcgc cgtgctgcgc
    21961 gtgatgcgaa tgctggagga caaaagcgga cgcaataatg aggtggtgaa gcagtatatg
    22021 gcaaaacgct ggagcgaaaa gttccacggc cagcgtgata tccaggcaca actgatgtcc
    22081 catcttgact acgcgctggc tcatactgac tggcatgcag agcgtcaggc gggcgacggt
    22141 gacgccatca gccgctggat gccatatgac aagcccgtgg tatcagcaca gaaagaactg
    22201 agcaaactgc ctgtctacca gcgggtttac cagagcctga aaacgcgggc gctgggcgtt
    22261 ctacctgctg acctcaatct gcgtgaccag gtagggccaa cctttgacca ggtctttaca
    22321 tctgccgatg acaacaaact ggttgttcca cagtttctta cccgttacgg cctgcaaagc
    22381 tattttgtaa aacagcgcga tgaactggtt gaactgacgg caatggattc ctgggtactc
    22441 aacctcaccc gcagtgtgaa atacagtgac gccgaccgcg cggaaatcca gcgccagttg
    22501 accgagcagt atatcagcga ctacaccgct acctggcgag ccgggatgga caatctgaat
    22561 atccgcaatt ttgagtccat tggacaactg accggggcgc tggagcaggt tatcagcggc
    22621 gaccagcctt tgcagcgggc gctgactgtg ttgcgtgaca acacacagcc aggcgtcttt
    22681 tctgaaaaac tttctgccaa agaacgggat gaggctctgg cagaaccgga ctaccagttg
    22741 ctcacccgcc tcgggcatga atttgccccg gaaaacagta ctctggcagt acagaaagac
    22801 aaagaaagca cgatgcaggc cgtgtatcag caactcaccg agttgcaccg ctatctgctg
    22861 gcaattcaga acgcgcctgt accagggaaa tcggcgctga aagccgtgca gttgcggctt
    22921 gatcagaaca gcagcgatcc gatattcgcc acccgccaga tggcaaagac actgcctgct
    22981 ccgctcaacc gctgggttgg cagactggct gaccaggcct ggcatgtggt gatggtggag
    23041 gccgttcact atatggaagt ggactggcgc gacagcgtgg tgaaaccgtt taacgagcaa
    23101 ctggcaaata actatccgtt taatccgcgt tctgcacagg atgcctcact ggatgccttc
    23161 gaacgcttct ttaaaccgga tggcatactg gataccttct accagcagaa cctgaagctg
    23221 tttatcgaca atgacctgag tctggaggat ggcgataaca gcgtcattat tcgcgaagat
    23281 attattgcgc aactgaaaac cgcgcagaaa atccgtgaca tcttcttcag taaacagaac
    23341 ggtctgggaa catcctttgc cgtggaaacg gtatcgctct ccggcaacaa acgccgcagt
    23401 gtactgaacc ttgacggcca gttagtcgat tacagccagg ggcgcaacta taccgcccat
    23461 ctggtctggc ctaacaatat gcgcgaaggc aacgaaagca aactgacgct catcggcacc
    23521 agcggcaacg caccgcgcag tatcagcttc agcgggccgt gggcgcagtt ccgccttttc
    23581 ggggccggac aactgaccgg agttcaggat ggcaacttta ccgtgcgctt tagcgtggac
    23641 gctggcgcga tgacctaccg tgtgcatacc gacactgagg ataacccatt cagcggtggg
    23701 ttattcagcc agtttggtct gtcagacaca ctgtactgag gaggcaggga tggatagtaa
    23761 cggactgaca caaactatcg ttaccggcag tgacccgcgc gggctgccgg aattcagtgc
    23821 catccgcgag gaaataaaca aagccagcca cccgtcacag cctgagctga actggaaact
    23881 ggtggagtcg ctggcactgg cgatttttaa agccaacggt gtggatttac acaccgccac
    23941 ctactatacg cttgcccgga cacggacaca gggactggcg ggattctgcg aaggtgcgga
    24001 actgctggcg gcaatggtaa gccacgactg ggataagttc tggccgcagg gcggcccggc
    24061 gcgtactgaa atgctggact ggtttaactc ccgaaccggc aatattctgc gtcagcaaat
    24121 ctcctttgcg gaatccgacc taccactgat ctaccgcaca gagcgggcat tgcagcttat
    24181 ctgcgataag ctccagcagg tggaactgaa gcgcgttccg cgcgtggaga atctgctcta
    24241 ttttatgcag aacacgcgta aacggcttga accccagctg aagagtaaca ctgagaacgc
    24301 cgcacagacc acggtcagaa cgctgattta tgccccggaa acacaggcat cttccacacc
    24361 agaagcggta gtgcctcccc tgcccggcct gcctgagatg aaagtggaag tgcgcagtct
    24421 gacagagaat cccccacagg ccagtgtgat aaagcaaggc agtacggtaa gagggtttat
    24481 cgcagggatc gcctgttcag tggctgtcgc ctcagcattg tggtggtggc aggtctatcc
    24541 ggtgcagcag caactgttac aggttaacga caccgctcag ggcgcagcaa cggtgtggat
    24601 ggcctcacct gaactcgaaa actatgagcg caggctgcaa caacttcttg atacctcccc
    24661 ggtacagccg ctggaaaccg ggatgcagat gatgcgtgtt gccgacagtc gctggccgga
    24721 aagcctgcaa cagcaacagg cctcgacaca atggaatgag gcactcaaaa cccgcgcaca
    24781 gagtagcccg cagttgcgtg gctggttgca gacccgccag gacttacatg cttttgcaga
    24841 tctagtgatg cagcgcgaga aagagggact aaccctttcc tatatcaaaa atgtcatctg
    24901 gcaggcggag cggggactgg ggcaggaaac acccgttgag tctctgttga cgcagtacca
    24961 ggatgcccgt gcgcagaagc agaatacaga tgcgctggaa aaacaaatta atgagcgact
    25021 cgaaggcgtg ttaagccgct ggctgctgct gaagaataac gtcatgccag aggcggcaac
    25081 cggtaccacg gccgaaaaat aattatttaa ttaaaggaga ttatcatggc aaatataagt
    25141 tatttatctt tgagtggaga gacacaaggg ctgatttcag caggttgctc aacgcttgat
    25201 tctgtcggaa acaaagcgca gccagaacat aaagatcaga tcatggttta tgcgttaatg
    25261 cattccatct caagaagtca aaatgtcaat catcatgaat taattatcac caaaccggtt
    25321 gataaatcct caccattgct ggcaaaagca cttagtgaca atgaaaaaat ggcaatatgt
    25381 gaatttatac tctaccgaac cagtaaagcg ggtatttacc agccttatta taaaataaat
    25441 ctgtcaaaag cccgaatatc aagtattgat tttgtcacac cgcatgctgt cctggaaaaa
    25501 gaactggaac cacaggagag aattgccttt atttatgaag atatttactg ggagcatacg
    25561 ttagccggta cgaatgcaat gagtaaatgg caggatcgtg tacagtaact tactgtaata
    25621 acataataaa ttactattgc ggagagataa aatggctggt ttaaggaata acaacaacac
    25681 acagaatgca cagtgggctg attatgtggg tgacattctt cgggggctca gccgataaac
    25741 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
    25801 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaat actcagccca
    25861 ataacatcat ttgattacat tcattttatc actacacatc cttcaggtat aaaagataca
    25921 ctcgcctggt tagttaacgc gggtaaatta atgactgagt ttgatgacaa tgggaaaatt
    25981 atatttaacc ttaatgcctt aaagtataca aaagcaagtt actttgaaat acttggagag
    26041 aaatatataa aaatcacaac atccagcccc tggcttttag aaaagctcgg taaatatatt
    26101 ttctcttcac gagcgcctca agttctggaa ctcgctattg gctggcgagg agctttatct
    26161 gaatcaataa aaggagttaa gttctgtatc tggttttcag tagcctggag aaccatagaa
    26221 tttattatga gttcagagcg agatctggtt aattttcttg gcgatttttc aatggatgtg
    26281 gccaaagcag ttatagccgg tggtgttgca accgctattg gaagcctggc ttcttttgcc
    26341 tgtgttagct ttggctttcc agtaattctt gtcggaggag caattttact gacagggata
    26401 gtgtgtacag ttgttttaaa tgaaatcgat gctcaatgcc atttatcaga aaaattaaaa
    26461 tatgcaatta gagatggact aaaacggcaa caggaacttg ataaatggaa aagggaaaac
    26521 atgactccat ttatgtatgt tcttaacact ccacccgtga tatgaggatt aaccatgcgt
    26581 ttacatgtta aattaaaaga gtttctttcg atgtttttta tggccatatt atttttccct
    26641 gcattcaatg cttctttgtt ttttaccgga gttaaacctc tatattcaat aataaaatgc
    26701 tccacggaaa tattttatga ttggagaatg ttgattctgt gctttggttt catgtcgttt
    26761 tcctttttaa atattcatgt aattttactt acgataataa aatcattctt aatcaaaaaa
    26821 acgaaggtag tcaatttcgc cacagacata acaatacagc taactcttat atttttacta
    26881 atagctatcg taatagcacc tttaatagct ccttttgtca caggatatgt caatgctaat
    26941 tatcacccct gcggtaataa tacggggatt ttcccaggag caatctatat taaaaatggc
    27001 atgaaatgta ataatgaata tatttctcgc aaggaagatt cagcagttaa ataattaaat
    27061 attctaaaac aaacagtttt taactccgga ttccatgtcc tttcttgcac tgtttttgtc
    27121 aagaaaaatt tattatgcaa agaattttgt attattgccg ccagaaattc ctggcggcag
    27181 atataattta ttattgatag cggttaacca aatcttttac catcgcttta taggtctttt
    27241 gcttaagttg catgatttgc tgctctgtca tatcagtcgt actggcttca ttcttgtcgg
    27301 gcaatggtgg aagcacttta tattcataat tcaccggcga ccaggtagcc cactcacctg
    27361 ttgccacatc caccagcgtg aaacgtacca ggtagcgcag ggagatagcg tcggtgagtt
    27421 tttcattttt atagtcggac catactgttg atttcagcgc agagtcatat tttcctgtct
    27481 gcaacgtatc ctggaaaaca ataatcgctt tctgatgccc ttttgcagcc acaaaacgca
    27541 gtgcctgcat ccagttcata ttctcagcac tggcaacatc ctcgttctca tttttatctt
    27601 tgtttttgtt acaagtcaga ggcttttgcc tgtccgggat accagagaat gtggaaacag
    27661 tataatattt tcgcatctcc tcctgcataa tggtttccgg tgcgcggttg ccggattgaa
    27721 caagtattac aggagaatta aggggaacgc ggaactcatc tccatcaagc gcagcttgaa
    27781 tatcctcttc ggatacggcc agagtggttt cattaccgaa taaatctttg tctgtcagtt
    27841 gctggggagt gtttttatta atattacttt gtgtgacttg agcttttttc ttcttttcat
    27901 caatagaaga ttcagaggca catcctgcaa gcagcgagaa taacacacat atgcaacaaa
    27961 gcttttttga attcataatt ggacactccc tcgcctgtca taatcatggt aatgtttagc
    28021 taacgatttt taattatata ctccacttga atcagaacaa acaaaaacaa cagtatatat
    28081 aaattcatta aatcaataga aaaatgaaaa tcataacatt caaatatatt taaattgaat
    28141 gattcgatag tatgattaaa tattaatgat atggtctacc atatgtgatt aacacattta
    28201 cagcataaca aaaccataga ccgaagaaat agcgggattt aaatgcaaaa aaaaaccgca
    28261 aaattaaaaa tcttgcggtt ctctgaactc attttcatga gtgaatttgg cggaacggac
    28321 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    28381 ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
    28441 acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagccg ctattttcag
    28501 cgccttttac aggtatttat gcccgccaga ggcagcttcc gcccttcttc tccaccagat
    28561 caagacgggc ttcatgagct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
    28621 ttgcctgacg tacaatgcgc tgaattgttg cttcaccttg ttgctgctgt gtctctcctt
    28681 ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc cagataaact tccgcaagga
    28741 tctgtgcatc gagtaatgcc ccgtgtagcg ttcgtttact gttatctatt tcgtagcggg
    28801 cacataacgc atcgaggctg ttgcgcttac caggaaacat tttcctcgcc accgcaaggc
    28861 tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact
    28921 cgtagtccat aaagccgata tcgaacgctg cgttatggat caccaactcc gcgccgcgga
    28981 tatagtccat gaactcatcg gctacttcgg caaacgtggg cttatcgagc aaaaattcat
    29041 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
    29101 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
    29161 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa
    29221 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt atcgttttac
    29281 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
    29341 atggttcgtg tctgggcaat ccaggacctg ggggttacgg cgctatttta cgctatcgcg
    29401 gacgcgagaa aacctttagc gcaggctaca cccgcaccac caacaaccgt atggagttga
    29461 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
    29521 acagccagta tgtccgccag gggatcactc agtggatcca tagctggaaa aaacgtggct
    29581 ggaaaaccgc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg
    29641 cattggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
    29701 acgaacgctg tgatgaactg gcccgtgctg cggcaatgaa tcccacactg gaagatacag
    29761 gctatcaagt cgaagtttaa gcctgtggtt tacgatattg ccgggtggct ccaaccgcct
    29821 ggcgaattcg tggcttgttt ttactctgtt tcatcggatt tagcgttaaa ggaatagtcc
    29881 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgtgca ttcaatagtt
    29941 ttccaccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgcagc acttcaaaat
    30001 tcaacaaaga gagccagtcc agttgccgca tcagagtaaa catccggctg ttatagggcg
    30061 aggttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg ggattgaagc
    30121 cactaatcac cagccagcca tcatcaatca atacccgatc ggcttcacgc aataaacgat
    30181 gcggatcggt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
    30241 caaaaggaag atgaagtggg tccgcctgta cctggacggg cattccttgc gcagaaacat
    30301 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
    30361 taagcagatg aaaaccatac attttagtga accacgggtt gagctggcgc tccagcgcct
    30421 tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg acagtttgag
    30481 ggactcttgc cggtttcatc acaaccttcc gtttcacact aagaggtaat ctatgaatct
    30541 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
    30601 ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccatta ccgccaataa
    30661 ctggcaaccg gaggccatat ttctcaccca ccatcatcac gatcacgttg gcggcgtaaa
    30721 agaactggtg gaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa
    30781 gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag
    30841 tgtaattgcc acaccgggtc acactttagg acatatctgt tacttcagta aaccttatct
    30901 attttgcggc gacacgctgt tttctggtgg gtgtggtagg ttgtttgaag ggacagcatc
    30961 acaaatgtat caatcactta ataagttaag tgcgttacct gacgatacat tggtatgttg
    31021 tgctcatgaa tatacgttat caaatatgaa gtttgctttg agtattcttc cgcacgattt
    31081 gtccataaat gattattatc gtaaagttaa ggagttacgg gcaaaaaatc aaataacact
    31141 acccgtaatt ctgaaaaatg agcggcaaat taatgttttt ttaagaacag aagatattga
    31201 tttaattaat gtaattaatg aagaaacatt attgcaacaa cctgaagaac gttttgcatg
    31261 gttaaggtca aagaaagata ggttctgata aaactttctt gtcatcggct ccgttcgccg
    31321 ttatgatcgg tcgtctttta agcaactatt gacacacaca tgaaggcaaa agcgatatta
    31381 ctcgcctctg tcctgctcgt gggttgccag agtaccggca acgttcaaca gcacgcacag
    31441 agcctttctg cagctggtca aggggaagca gcaaagttta caagtcaggc acgatggatg
    31501 gacgatggga cgtctatcgc gccagatggt gacttgtggg ctttcattgg cgacgagcta
    31561 aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga aacagaaata tttacgcaat
    31621 aagagctatc tccacgatgt aactttacgg gcagagccgt atatgtactg gatagccggg
    31681 caagttaaaa aacgtaacat gcctatggaa ctggtactac tacccatagt ggagagcgct
    31741 tttgatcctc acgcaacgtc tggcgccaat gccgcaggca tctggcagat cattccgagc
    31801 acggggcgca attatggttt gaaacagacc cgcaattatg acgcgcgtcg cgatgttgtt
    31861 gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga acaagatgtt tgacggcgac
    31921 tggcttctga ccgtagcggc ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa
    31981 acgaacaaag cgcgtgggaa atccacggac ttctggtcgt taccgttgcc gcaggaaacg
    32041 aagcagtacg tgcctaaaat gctggcattg agtgatattc tcaaaaacag caagcgttat
    32101 ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg cgcgtgtgca cctgagcagc
    32161 ccggttgaaa tggcgaaggt tgcagatatg gcggggattt ccgtcagcaa gctgaagaca
    32221 ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg gtccgcaata cgtgatggtg
    32281 ccaaagaagc atgcagatca actgcgtgaa tctctcgctt caggcgaaat tgctgctgta
    32341 cagtcgacgc tggttgccga caatacgccg cttaacagcc gtgtttacac cgtacgctct
    32401 ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag
    32461 tggaacaaac tgcgcggatc taagctgaag ccaggccaaa gtttgacgat tggtgcaggc
    32521 agtagcgcac agcgactggc aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc
    32581 gattcgcttt caagcattgc taaacgccac ggcgtgaaca tcaaagatgt gatgcgctgg
    32641 aacagcgaca ctgcgaatct gcaaccaggc gacaagctga cgttgtttgt gaaaaacaac
    32701 aacatgccag attcctgaca aaccagataa taaaaaggca ccgattcccc cggtgccttt
    32761 tttatttatg ctgctttatg tgcttctacc atgatgatat cactggtgaa agagccatca
    32821 ttctgcaagg caaaatacgt tctcacctct gtcgatgcgc tctgttggta aatgcgaata
    32881 gcgtctacta acgcttctag cgtacgcatt ctcgcgaccc atgaagaaaa ttccagcggt
    32941 aacttatctg taattaaatt atcaactatc agattggctt cattgattaa cctcaaccac
    33001 tcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac cgtctgtaac
    33061 cagatgtcgc gcactggatg acccggagac attacgtcca tcacaatcat cctaccgcca
    33121 ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca gtgatgggca
    33181 gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact ttcggcataa
    33241 ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cagcttgtgc cacgacatcc
    33301 agcatttggg cagataagtc atacgccacc accgcgctca cgttctgcgc agcgacaaag
    33361 ctggcatgcc ctgctccgca gcccatatca agtacacttg catcaggata atcagccaga
    33421 cgcaccgcca ggcgctgtaa atctcgcccg gatgcatgta cggtactggt taaatactca
    33481 ctggcctgcg agctaaactg cttttctacg tggtcatggt gggattgtgt tgtcattgcg
    33541 ctgtcctttt gttgtttgag tataaaagga cagcgccaaa cgttggtctg ccctgtggca
    33601 gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt agcggattgt
    33661 gatcggaagc gcgcgtaacc aatacagaag cttcgctgac gttcagacca cggtagaaaa
    33721 caaaatcgag cgggcggcca aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc
    33781 gcagcgacat ttcccgcgca aagcgatata acgcgttcat ccttctacgg ctccaggcat
    33841 tgaaatctcc tgccataatg accgggccgc tgtggtgagc tatctgatca ccaataggaa
    33901 gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt atattaacca
    33961 ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac ttcgccagac
    34021 gcaaaatggg ttctcgttca cgtaacgggc agcaatacac tggatgcgcc gccgaaaggg
    34081 tcattacgcc agaaggatgt tgtggcagta cgaaagcggg tacctgatcg gcggcaagat
    34141 agttagcggt cgcaaactgt actaactctg gcgttgtctg cgcttcctgc aataacacca
    34201 gatgtgcatc tttgccgtag ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt
    34261 atatgttcca caccaggatc cgaatacgct cttcggtact taacggttct ccgggtggta
    34321 atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctttccgca ggttgtccgg
    34381 caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc tctattacgt
    34441 caacccttcc gcactcggga gggttctact gttataggga ttttagctca ctctttcaac
    34501 gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaattt tgctcattat
    34561 ggagaacatt aaatcgcatg acatatcacg catcctgatg gtttaatgac aaataaattc
    34621 aaaatatctc ttctattttg caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc
    34681 catttttgtt tcggtcgtcg aaagcggcag ctttagccgg gcagcggaac aattagggca
    34741 agcaaactca gcggtaagcc gggcggtgaa aaagctggag atgaaacttg gcgttagcct
    34801 gcttaatcgg accacgcgac aacttagcct gacggaagaa ggcgagcgtt attttcgtcg
    34861 cgtacagtca attttgcagg agatggcatc ggcagaatca gaaattatgg agacgcgtaa
    34921 tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc actttctgat
    34981 gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttgtcgc tagtctcctc
    35041 cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt
    35101 aacggattcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc
    35161 ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa agcaacatat
    35221 ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagccc gtagcgatgg
    35281 acaattacat gaggtgaagt acggtttgtc atccaatagt ggggaaacac tgaaacagct
    35341 ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc
    35401 tcgcggagaa ttggtggagt taatggcaga taaagtgttg ccagtggaaa tgccattcag
    35461 tgcagtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta tcgatttcct
    35521 tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat cccattcagg
    35581 agccagacct tcctggctaa ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt
    35641 atcttcggca tcaagctgta aattttgtgc cttaagatta ctttccaggt tttcacgttt
    35701 agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa tcacttgtgc
    35761 cggagtcgca ttgtgtttag ctgcgatacg agcaataacc tcatctttca gggccttacc
    35821 atacgccagc gtcatatagg aagtaatatg gatgccgtgc tgtttagccc agacaaccac
    35881 tttacggttt tgcagataag gagagagttc aatctggtta gtagcgatgt tttcagcgcc
    35941 aacagcagca atcgtttttt ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg
    36001 tgtcagacct tgttttttgg cttccagcag cgcctgcata aactcttcaa cagagacttc
    36061 atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac gcaatttttg
    36121 caggctctct ttcagacttg ggatcaattt gtctttgctg agattttcaa tccagatttt
    36181 agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct gacctactgc
    36241 ggcttcgtta tcatagattt gtgcggtatc aattgcgcga taaccaagtt caagcgccgt
    36301 tttcacagat gaaataacaa cgtcgtcttt cagacggaaa gtacctaaac caaatgcagg
    36361 gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc
    36421 agactgttgc ggaaaaagag cgtaaaacgc agaggatttt tgcgattctg gcaataatag
    36481 aaatgtagac gaaaaaaaac ctcgagcgat aactcgaggc ttcttaata
//
LOCUS       DAHBHC010000051        35347 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHBHC010000051 DAHBHC010000000
VERSION     DAHBHC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.051"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            20..703
                     /locus_tag="M2A35_002954"
     CDS             20..703
                     /locus_tag="M2A35_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HCB7199223.1"
                     /translation="MFNAFWQERRFLPSLVQQELVIGGRLGKKSGLGVYDWRAEREAV
                     VGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALAIRLARPVVVVDKMAGK
                     VVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWRTVAMIINEALDALQKG
                     VASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQHHYGEERYRPCSLLRQR
                     ALLESGYES"
     gene            693..1115
                     /gene="paaI"
                     /locus_tag="M2A35_002955"
     CDS             693..1115
                     /gene="paaI"
                     /locus_tag="M2A35_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HCB7199224.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            1115..2320
                     /gene="paaJ"
                     /locus_tag="M2A35_002956"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             1115..2320
                     /gene="paaJ"
                     /locus_tag="M2A35_002956"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HCB7199225.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            2347..3660
                     /gene="paaF"
                     /locus_tag="M2A35_002957"
     CDS             2347..3660
                     /gene="paaF"
                     /locus_tag="M2A35_002957"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HCB7199226.1"
                     /translation="MITNTKLDPIETAPVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            3761..4711
                     /gene="paaX"
                     /locus_tag="M2A35_002958"
     CDS             3761..4711
                     /gene="paaX"
                     /locus_tag="M2A35_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HCB7199227.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            4693..5283
                     /gene="paaY"
                     /locus_tag="M2A35_002959"
     CDS             4693..5283
                     /gene="paaY"
                     /locus_tag="M2A35_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HCB7199228.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHMGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            5515..6375
                     /gene="pdxI"
                     /locus_tag="M2A35_002960"
     CDS             5515..6375
                     /gene="pdxI"
                     /locus_tag="M2A35_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HCB7199229.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            6439..8745
                     /locus_tag="M2A35_002961"
     CDS             6439..8745
                     /locus_tag="M2A35_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCB7199230.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            8916..9521
                     /locus_tag="M2A35_002962"
     CDS             8916..9521
                     /locus_tag="M2A35_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCB7199231.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            9521..10417
                     /locus_tag="M2A35_002963"
     CDS             9521..10417
                     /locus_tag="M2A35_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCB7199232.1"
                     /translation="MLEKSLATLFALLILATLINHFLVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            10433..12190
                     /locus_tag="M2A35_002964"
     CDS             10433..12190
                     /locus_tag="M2A35_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCB7199233.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGMHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            12180..13496
                     /locus_tag="M2A35_002965"
     CDS             12180..13496
                     /locus_tag="M2A35_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCB7199234.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARCSHIVLKEDHKAMLKLWENR"
     gene            complement(13547..14152)
                     /gene="azoR"
                     /locus_tag="M2A35_002966"
     CDS             complement(13547..14152)
                     /gene="azoR"
                     /locus_tag="M2A35_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCB7199235.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            14353..18255
                     /gene="hrpA"
                     /locus_tag="M2A35_002967"
     CDS             14353..18255
                     /gene="hrpA"
                     /locus_tag="M2A35_002967"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCB7199236.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            18527..19327
                     /locus_tag="M2A35_002968"
     CDS             18527..19327
                     /locus_tag="M2A35_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCB7199237.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            19524..20963
                     /gene="aldA"
                     /locus_tag="M2A35_002969"
     CDS             19524..20963
                     /gene="aldA"
                     /locus_tag="M2A35_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCB7199238.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(21005..22006)
                     /gene="gap"
                     /locus_tag="M2A35_002970"
     CDS             complement(21005..22006)
                     /gene="gap"
                     /locus_tag="M2A35_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCB7199239.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            22195..22725
                     /gene="cybB"
                     /locus_tag="M2A35_002971"
     CDS             22195..22725
                     /gene="cybB"
                     /locus_tag="M2A35_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCB7199240.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            22970..23143
                     /locus_tag="M2A35_002972"
     CDS             22970..23143
                     /locus_tag="M2A35_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199241.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(23215..23364)
                     /gene="hokB"
                     /locus_tag="M2A35_002973"
     CDS             complement(23215..23364)
                     /gene="hokB"
                     /locus_tag="M2A35_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCB7199242.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            23763..25403
                     /gene="trg"
                     /locus_tag="M2A35_002974"
     CDS             23763..25403
                     /gene="trg"
                     /locus_tag="M2A35_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCB7199243.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(25441..26364)
                     /locus_tag="M2A35_002975"
     CDS             complement(25441..26364)
                     /locus_tag="M2A35_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199244.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            26581..27924
                     /locus_tag="M2A35_002976"
     CDS             26581..27924
                     /locus_tag="M2A35_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCB7199245.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            28149..29804
                     /gene="opgD"
                     /locus_tag="M2A35_002977"
                     /gene_synonym="mdoD"
     CDS             28149..29804
                     /gene="opgD"
                     /locus_tag="M2A35_002977"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCB7199246.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            29944..30168
                     /locus_tag="M2A35_002978"
     CDS             29944..30168
                     /locus_tag="M2A35_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCB7199247.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            30231..30767
                     /gene="rimL"
                     /locus_tag="M2A35_002979"
     CDS             30231..30767
                     /gene="rimL"
                     /locus_tag="M2A35_002979"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCB7199248.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(30762..31742)
                     /locus_tag="M2A35_002980"
     CDS             complement(30762..31742)
                     /locus_tag="M2A35_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCB7199249.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            31866..32858
                     /gene="tehA"
                     /locus_tag="M2A35_002981"
     CDS             31866..32858
                     /gene="tehA"
                     /locus_tag="M2A35_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCB7199250.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            32855..33448
                     /gene="tehB"
                     /locus_tag="M2A35_002982"
     CDS             32855..33448
                     /gene="tehB"
                     /locus_tag="M2A35_002982"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCB7199251.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            33751..34419
                     /locus_tag="M2A35_002983"
     CDS             33751..34419
                     /locus_tag="M2A35_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCB7199252.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(34437..34942)
                     /gene="tnpA"
                     /locus_tag="M2A35_002984"
                     /pseudo
     CDS             complement(34437..34942)
                     /gene="tnpA"
                     /locus_tag="M2A35_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            34950..>35347
                     /locus_tag="M2A35_002985"
     CDS             34950..>35347
                     /locus_tag="M2A35_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7199253.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLG"
BASE COUNT         8801 a         8149 c         9261 g         9136 t
ORIGIN      
        1 ttttgctgtc acctgttcgg tgtttaacgc tttctggcag gagcgtcgtt ttttaccttc
       61 gctggtgcaa caggaactgg tgattggtgg acggttgggc aagaaaagtg ggctgggcgt
      121 gtacgactgg cgcgcggaac gtgaggcagt tgttggcctg gaagcggtaa gcgacagttt
      181 tagcccaatg aaagtagaaa agaaaagtga cggtgtcacg gaaattgacg atgttttatt
      241 gattgagaca caaggcgaga cggcacaggc gctggcaata cgactggcac gcccggtggt
      301 agtggtcgac aaaatggcgg gcaaggtggt gaccattgct gcggcagcgg tgaacccgga
      361 ctcagcgacc cgcaaggcca tttattacct gcaacagcag ggcaaaacag tgctgcaaat
      421 tgcagattac ccaggaatgc tgatttggcg aacggtagca atgatcatca atgaagccct
      481 tgatgcgctg caaaaaggcg tggcctctga acaggatatc gataccgcca tgcgtcttgg
      541 ggtgaattat ccatatggcc cgcttgcctg gggagcgcaa cttggctggc agcgaatatt
      601 aaggctcctg gaaaatctac aacatcacta tggcgaagaa cgctatcgcc catgttcatt
      661 gctgcgccaa cgagcgcttc tggagagcgg ttatgagtca taaggcctgg caaaatgccc
      721 atgcaatgta tgagaacgat gcctgcgcca aagcgcttgg catcgacatt atctcaatgg
      781 atgaaggctt tgctgtagtg accatgaccg tcactgcaca aatgcttaat ggtcatcaaa
      841 gttgccacgg cgggcagcta ttttcactgg ctgatactgc ctttgcctac gcttgcaata
      901 gccaggggtt ggcagctgtc gcttctgcct gcacgattga ttttttacgt ccaggctttg
      961 ccggagacac cttaactgcg actgcgcagg tacgtcatca gggcaagcaa accggtgttt
     1021 acgacatcga aattgttaac caacaacaaa aaacggttgc gctgtttcgc ggtaaatctc
     1081 accgcatcgg cggcaccatt acaggagaag cctgatgcgt gaagccttta tctgtgacgg
     1141 aattcgtacg ccaattggtc gctacggcgg ggcattatca ggtgttcggg ctgacgatct
     1201 ggctgctatc cctttgcggg agctgctggt gcgaaacccg cgtctcgatg cggagtgtat
     1261 cgatgatgtg atcctcggct gtgctaatca ggcgggagaa gacaaccgta acgtagcccg
     1321 gatggcgact ttactggcgg ggctgccgca gagtgtttcc ggcacaacca ttaaccgctt
     1381 gtgtggttcc gggctggacg cactggggtt cgccgcacgg gcgattaaag cgggcgatgg
     1441 cgatttgctg atcgccggtg gcgtggagtc aatgtctcgg gcaccgtttg ttatgggcaa
     1501 ggcaaccagt gcattttctc gtcaggctga gatgtttgat accactattg gctggcgatt
     1561 tgtgaacccg ctcatggctc aacaatttgg aactgacagc atgccggaaa cggcagagaa
     1621 tgtagctgaa ctgttaaaaa tctcgcgaga agatcaagat agttttgcgc tacgcagtca
     1681 gcaacgtacg gcaaaagcgc aatcctcagg cattctggct gaggagattg ttccggttgt
     1741 gttgaaaaac aagaaaggcg ttgtaacaga aatacaacat gatgagcatc tgcgcccgga
     1801 aacgacgctg gaacagttac gtgggttaaa agcaccattt cgtgccaatg gggtgattac
     1861 cgcaggcaat gcctccgggg tgaatgacgg agccgctgcg ttgattattg ccagtgaaca
     1921 gatggcagca gcgcaaggac tgacgccgcg agcgcgtatc gtagccatgg caaccgccgg
     1981 ggtggaaccg cgcctgatgg ggcttggtcc ggtgcctgca actcgccggg tgctggaacg
     2041 tgcggggctg agtattcacg atatggacgt gattgaactg aacgaagcgt tcgcggccca
     2101 ggcgttgggt gtactacgcg aattggggct gcctgatgat gccccacatg ttaaccccaa
     2161 cggaggcgct atcgccttag gccatccgtt gggaatgagt ggtgcccgcc tggcactggc
     2221 tgccagccat gagctgcatc ggcgtaacgg tcgttacgca ttatgcacca tgtgcatcgg
     2281 tgtcggtcag ggcatcgcca tgattctgga gcgtgtttga gcatatcaac ctgcgagtac
     2341 cctacaatga taaccaatac aaagcttgac ccgattgaaa ccgcgcctgt tgatgagtta
     2401 caggcgttgc aaacacagcg tctgaaatgg acgctcaaac acgcgtatga aaatgtcccg
     2461 atgtatcggc gcaaattcga cgcagcaggc gtacatcctg atgatttcag ggaactttca
     2521 gacctgcgta aatttccctg taccaccaaa caggatctgc gcgacaacta tccctttgac
     2581 acctttgccg tgccgatgga acaagtggtg cgcattcatg cttcttcggg aaccacaggt
     2641 aaaccgacag ttgtcggtta tacgcaaaac gatattgata actgggccaa tattgtagcg
     2701 cgttctttgc gtgcggcagg gggctcgccc aaagacaaaa ttcatgttgc ctatggttac
     2761 ggcctgttta ctggtgggct gggcgcgcac tatggtgccg aacgtctggg cgctacggtg
     2821 atcccgatgt ctggcgggca gacggaaaaa caagcgcaac tgatccgcga ttttcaacca
     2881 gatatgatca tggttacgcc atcttattgc cttaacctga ttgaagagct ggagcggcag
     2941 ttgggcggtg atgccagcgg ttgctcgctg cgggttggcg tgtttggtgc tgagccgtgg
     3001 acacaggcca tgcgtaaaga gattgaacgg cgtctgggga tcaccgcact ggatatttat
     3061 ggcctgtcag aagtgatggg gccgggggtg gcgatggagt gtctggaaac taccgacggt
     3121 ccaactattt gggaagatca tttctatcct gaaattgtta atcctcatga cggcacaccg
     3181 cttgccgatg gtgaacatgg cgaactgtta ttcaccacgc tgaccaaaga agcattgccg
     3241 gtcattcgtt accgcacgcg tgatttaacc cgactgttac caggaacggc gcggactatg
     3301 cgccgcatgg atcgcatcag cggacgcagc gatgacatgt tgattattcg cggtgtaaat
     3361 gtctttccgt cacaactgga agaagagatt gtcaaattcg aacatttatc gccgcattac
     3421 caactggagg tgaaccgccg ggggcatctt gattcacttt ctgtgaaagt ggagttgaaa
     3481 gaaagtagcc tgacattgac gcatgagcag cgttgccagg tatgccacca gttgcgccat
     3541 cggattaagt cgatggtggg gatctctacc gatgtgatga tcgttaactg tggcagtatc
     3601 ccgcgttcag aaggcaaggc gtgtcgggtg tttgatctgc gcaatattgt tggtgcctga
     3661 cgtgtcgtct ggccctggtg aggtaaagcg ccagggccag aagtcgatac gacctgtgct
     3721 atgattcata aatcacaaca ataacaacag actgaatcga atgagtaaac ttgatacttt
     3781 tatccaacat gctgtaaacg ctgttcctgt cagtggcaca tctttgatct cctctctgta
     3841 tggtgattct ctttcccatc gtggtggtga aatctggctg ggtagtctgg ctgctttgct
     3901 ggaagggctg ggatttggtg agcgtttcgt gcgcactgct ttgtttcgtc ttaataaaga
     3961 aggctggctg gatgtttccc gcatcgggcg acgcagtttc tatagcctca gtgataaagg
     4021 cttgcgcctg acgcgacggg cagaaagtaa aatttatcgc gcagagcaac ccgcatggga
     4081 tggtaaatgg ctcctgttgc tctcggaagg tttagataaa gcgacgctgg ctgatgtcaa
     4141 aaagcagttg atctggcaag gttttggtgc actggcgccc agcctgatgg catcgccgtc
     4201 gcaaaaactg gccgatgtac agacactttt gcatgaagcg ggtgtggcgg ataacgtgat
     4261 ttgttttgaa gcgcaaatac cactggcgct ttctcgtgca gcactgcgtg ccagagtaga
     4321 agagtgctgg catttaactg aacaaaatgc catgtacgaa acctttattc agtcattccg
     4381 cccgttggtg cagctgttaa aagaggcagc agacgagtta accccggagc gtgcatttca
     4441 tattcagctt ttactgatcc atttttatcg ccgtgtcgtc cttaaagacc ctttgttgcc
     4501 ggaggagttg cttccggctc actgggcagg gcatacggcg cgtcagctgt gtatcaacat
     4561 ttatcagcgc gtagcgcctg ctgctttagc gttcgttagt gaaaaaggtg aaacctcggt
     4621 cggtgaactg ccttcgccgg gtagcctgta ttttcaacgt tttggcggtt tgaatattga
     4681 acaggaggcg ttatgccaat ttatcagata gacggtctga ctccggttgt gccagaagag
     4741 agttttgtcc atccgacagc ggtattgatc ggcgatgtta ttctcggcaa gggtgtttac
     4801 gttgggccaa atgccagcct gcgtggcgat tttggtcgta tcgtggtgaa agatggcgcg
     4861 aacattcagg ataattgcgt tatgcacggt tttcccgagc aggatactgt cgttgaagaa
     4921 gatgggcata tgggtcatag cgctatcctt cacggctgca ttatccgccg caatgcatta
     4981 gtgggaatga acgcggtagt gatggatggt gcggtgattg gcgagaacag cattgttggc
     5041 gcatccgcat ttgtgaaagc caaagcagaa atgcctgcca attacctgat tgtcggcagc
     5101 ccggcgaaac tgattcgtga gcttagtgag caggagctgg catggaaaaa gcagggtacg
     5161 catgagtatc aggtgctggt gacacgctgt aagcagacgt tacatcaagt cgaaccattg
     5221 cgggaagttg aacctggcag gaaacgcctg gtatttgatg agaatctgcg accgaaacag
     5281 taacgtccag atactgaacg ttctaatttt tagcatgtga tacctaaacc gcaacgctga
     5341 tacaggttgc ggttttttta ttgccgaatg cggcgcttta cgcgttttgt tttggtgtct
     5401 ttcaggacaa tagagcaact catccagtaa tcttgtttac gccttcgcat tatttatctc
     5461 ttttcgtttc tatactgatt tttctcaatc cgttttatta cagggcaggg tgcgatgagc
     5521 agcaatacat ttactctcgg tacaaaatcc gttaaccgtc ttggttatgg cgcgatgcaa
     5581 ctggcaggtc ctggggtttt tggtccccca agagatcgcc acgtcgctat aaccgtgctg
     5641 cgcgaggcgc tggcattggg cgtcaatcac attgatacca gcgactttta tggtccgcac
     5701 gtcaccaatc agattatccg cgaagcgctt tatccttact ctgacgacct gacaattgtc
     5761 actaaaattg gtgcgcggcg tggagaggac gcatcctggt tgcccgcatt ttctccggca
     5821 gagctgcaaa aagcggtgca cgataatcta cgtaatctcg ggctggacgt actggatgtg
     5881 gttaacttgc gcattatgat gggggatggt catggcccag cggaaggatc gattgaagcc
     5941 agcctgaccg tgctggcaga gatgcaacaa caaggcctgg taaaacatat tggcctaagc
     6001 aacgtcacac cgacgcaggt tgcagaggcg cgcaagattg ccgaaatcgt ctgtgtgcag
     6061 aacgaataca acattgctca tcgtgctgat gacgcaatga ttgatgcttt ggctcgtgac
     6121 ggcattgcct acgtgccgtt cttcccgctc gggggcttta caccgctgca atcctctacg
     6181 ctgtcggatg ttgctgcgag cctgggtgca acacccatgc aggtggcgct ggcgtggctg
     6241 ttacaacgtt caccgaatat tttgctgatc ccagggacgt cttctgttgc gcatttacgg
     6301 gagaatatgg ctgctgaaaa attgcatctt tctgaggaag tgttgtctac gttggatggt
     6361 atttcgcgag aataacgaat atacaaaagg gaaagatgca tttccctttt gtttcttttt
     6421 taatggcatg gagtgcatat gtttaaggcc aggatttgcg gctggatagg tttacttccc
     6481 ctgtttatgc tgagtcttcc agtacaggcg gaacttcgat gtgttgcaaa tacagttgat
     6541 attgaaccat ttttttctgc ggctaccgct gaagataaac aacaagttga acaagctatc
     6601 aacagtagcg tgaatcttgt ccccttcggt ttatctgcat cggactggaa agtgcatcgt
     6661 ggcgatttag tggtagaagg taatatagag agtaatcaaa aattgattgt tcttggtaat
     6721 ttgacagtca aaggtaatat ttccactttc tctctttcta atccatgggt tattctcggt
     6781 aacgtgacgg caacgaatat agttacggat tcaccactgt taattaccgg ttcgataaat
     6841 gcaagtggac tggtatttat cgactcatat tacgataatc cgtcttcgat taaggggagt
     6901 attaatgcgc gtggaatatt tatcaatgac ataattgcac ctgttgttgc gtcttcgaca
     6961 aatagtgaat tcatggtccg tgcgagtgac aaaaatgaca ctgaaaatgt caaaaaagcg
     7021 ctgatgataa taaatcctga tgcatattat tggggtctaa taaatgatga agatgctctg
     7081 aaagaaattt ttaagcgaag caatattcgc atggcaggga atgtctgtaa tcagatgaaa
     7141 aaagaagcgc tgtttcgccc taagccttct cctgagttag tgcaggaatt gcaaatgctg
     7201 gatgaaggca acgttgctgc atttgaagga cgagatattg cgacatttga tcttgccata
     7261 atgcgaactc tcccaaggct taaaggaatt tcagctaacc tacgcaaaca acttattaat
     7321 agcaatgatg aacaaacgat tgaaagtatg gcgagatata tgcctgacaa tgagattctg
     7381 gagttgaccg atcagcaatt gggttaccag cctgttgttc tggggttgct cgaccgtgaa
     7441 ccgctctctg tcgaaataat gacgcgaatg agtcgtctac ctgatggtgt cggtccgttg
     7501 aatcttgcgc tacgtgaaaa tctccctctg gatatcgtaa tgactctggc gaaaagagat
     7561 tgggatatga tcattcagga gctttataaa gatgcgtggt tattgcctga atccattatt
     7621 gatggctata tccgcagtga tgattcctct attcgtcagg tcggtgctgg aggacaactc
     7681 acctacaatc aggcaatgca gttggcgaac gattcatcaa acaatgttgt cacaagctta
     7741 gcgttcaagc tggcagagat gaaacaccat ggtcaattat tgcggatgac gccacaagag
     7801 agtgacaaag ttgcagccta tttataccaa aaattcgaga atgacgatga tctaatacgt
     7861 gtgttatttt tagcattgcc agataactta cagtttaatt ttgttaaaag gatggagaaa
     7921 aaatcccctg cctacttttg ctgtcgggat atgcaggtaa ttcactctga cgctgctcta
     7981 caacggttat tgacacgttt caacgatcct gaggggtgga gtaatctggc gaaaaatcag
     8041 tatctgagca cgtcgatgaa acaaaaaatt tggcaacgtg ctttatcgca tcggaaaaat
     8101 aatccgaaag cagattcagc tgcgtatgaa accagcgctg atatgatttt gtctgagctt
     8161 atcagccacg gtgaagttga tgatcagatg ctattaaatg ccaccgccct gatacgtttg
     8221 gaagactggg actttttaga gagtgcatta gttagttggg ataatttacc cgctgttgtc
     8281 ctcaaggaat tacagcaaaa tacgccacgc aatgatattt gggcgaagtt ttttctgagg
     8341 caggaaaaca gctcccgtgc gcaggttgat gaagcattac gcgtctatta tgcactcgac
     8401 cccgatgcgt tagcacaact ggatgtactg gcaaaacaac cggatcgtat atggtggagt
     8461 acactggcga aaagcaatct cacatttttc aagttcggcg cacttaacaa ccgccacaca
     8521 ccgcctgcag tactggcggc agaaattgat cccgagtggt ggattgtggc gatgaataat
     8581 ccccgttttc cagttgatgt attaaaggcg aggctgaaac gtgatccttt gctggcgtta
     8641 gagcttgtta accctgaact ggatttagtc cgccagctgg cgcttaacgg taagacgcgc
     8701 gcaatacggg aacaagcgat gagaaaactt gatgagttgt attgatccac aacgtaatat
     8761 ttcttaaccc tttgattaat tggggataat tattcctgac agtgagtccc caataccttg
     8821 atatattctg aatttttaat gaagcggcgt gttgcgatat ctccgtcagg ggaattgatg
     8881 caccatagcg caaaccgaat tatcaaggat tgataatgac gctctaccag ataaaaccgc
     8941 tctttcagtc gctgttaagg ccgacgatgt tttggcttta caagcaccac gttacagcga
     9001 atcacatcac tcttgctgca ctggcgcttt ctcttcttac cggattgctg ttgatgttgg
     9061 tggcacaacc catcctcttt atgctattgc ccatcgtgct ttttatccgt atggcgctca
     9121 atgcgctgga tggcatgttg gcgcgtgagt gcaatcagca aacacggctt ggggcaattt
     9181 tgaatgagac tggtgatgtt atttccgata ttgcgctcta tttaccattt ttatttttac
     9241 cggaaagtaa cgcatcactc gtgatactca tgttattttg caccatattg accgagtttt
     9301 gcggtttact cgcgcagacg attaacggtg ttcgcagtta tgccggtcca tttggcaaaa
     9361 gcgatcgcgc gttaatattt ggtctgtggg gtctggccgt tgccatttat ccacagtgga
     9421 tgcagtggaa taatctttta tggagcattg cttcaattct gcttctctgg actgcgatta
     9481 atcgttgtcg gagtgtgctt cttatgagcg ctgaaagata atgctggaaa aatctctggc
     9541 aacacttttc gcgttgttaa ttttagcaac gctgataaac cattttctgg tatggcggtt
     9601 accggagaga aaagaggatg aagttacatt acgtattcgt acctggtggg gcatcgtcat
     9661 ttgtttttca ctggtgattt caggtccacg ctggatgacg ttgacgtttt ttgcgctgat
     9721 aagttttctg gcattgaaag aatattgtac gcttatatct gtacattttc cgcggtggtt
     9781 atattgggtt attcctctta actatttgtt tatcggtttt aattgctttg agctgtatct
     9841 attatttata cctttggctg ggtttctaat attagccacc gggcaagttt ttgtggggga
     9901 cccctccggt tttctgcata ccgtgagcgc cattttttgg ggctggataa tgaccgtttt
     9961 cgccttgagt catgccgcct ggttattaat gttgccaacc acaaatatcc agggcggggc
    10021 gttactggtg ttatttcttt tggcattaac cgagtcaaac gatattgcac agtatttatg
    10081 gggaaaatcc tgcggcagaa gaaaagtggt ccccaaagtc agcccgggaa aaacgctgga
    10141 aggtctggtg gggggcgtca tcaccaccat gattgcgtca ctgattattg gaccgttgct
    10201 gacaccgcta aatacattac aggcattatt agcgggtttg ttaattggta ttagtggttt
    10261 ttgcggcgat gtcgtgatgt cagccatcaa acgagatatt ggtgttaaag atagtggaaa
    10321 actattacca ggacatggcg gactccttga caggattgac tcattaattt tcaccgctcc
    10381 ggtatttttt tattttattc gctacagctg ttactgaagg aaaatagaga aaatggaaaa
    10441 ttcacgcatc cctggggaac atttttttac caccagtgat aatacagcgt tgttttatcg
    10501 gcactggccc gctttacagc ccggggcgaa aaaggtcatc gtcttatttc atcggggtca
    10561 tgaacattct ggtcgtctac aacatctcgt tgatgaactg gcgatgccag atactgcttt
    10621 ttatgcatgg gatgcccggg ggcatggaaa aacttcgggg ccgcgtggtt atagcccatc
    10681 tcttgcgcgt tcagtgcagg atgtcgatga atttgtccgt tttgctgcca gcgacagcca
    10741 ggttggactg gaagaggtgg ttgtgatcgc gcaaagcgtc ggcgcagtgc tggttgccac
    10801 atgggttcat gattatgcgc ctgcaattcg cgggctggtg ctggcttctc cggcctttaa
    10861 ggttaaattg tatgtgccgc tggcacgtcc tgcgctggcg ttatggcatc gtctgcgtgg
    10921 tctgtttttt attaattcct atgtgaaagg acgctatttg acccacgatc ggcaacgggt
    10981 ggcgagtttc aataatgatc cgctgatcac acgggcgatt gccgtcaata tcttgctcga
    11041 tctctacaaa acgtctgaac gtattattag agatgcggcg gcgattacgc tccccacgca
    11101 acttctgata tcaggcgatg actatgtggt gcatcgccaa ccgcagattg atttttatca
    11161 gagattacgt agccctctga aagagctgca tctgctgcca ggcttttatc acgacacgtt
    11221 gggtgaagag aacagggcgc aggcatttga aaaaatgcaa agctttatta gtcgtttata
    11281 tgctaacaag tcacaaaaat ttgattatca gcatgaagac cgcactggac catcagcgga
    11341 tcgctggcgg ctcctttcag gtggacccgt gccattatcg ccggttgatt tggcgtatcg
    11401 ctttatgcga aaggcgatga aattgttcgg ggcgcactct gcgggcatgc atctcggaat
    11461 gagcaccggc tttgattcag gcagttccct ggattatgtc tatcaaaatc aaccgcaagg
    11521 cagtaacgca ttcgggcgct taattgacaa aatctacctg aacagtgttg gctggcgcgg
    11581 tattcgccag cgtaaaaccc atttacaaat actgattaaa caagccgttg ccgatctcca
    11641 cgccaaaggt ttagccgtcc gcgtggttga cattgccgca gggcatgggc gctatgtact
    11701 ggatgcgctg gcaaacgagc ctgccgtaag cgatattttg ttacgtgatt acagtgagtt
    11761 aaatgttgca caggggcaag cgatgattgc ccaacgggga atgtctgggc gggtgcgttt
    11821 tgaacagggc gatgcgttta acccggagga actcagcgcg ttaactccgc ggcctacgct
    11881 ggcgattgtc tctggcctgt atgagctttt tcccgaaaat gagcaggtaa aaaactcact
    11941 cgcaggtctt gccaatgcca tcgaaccggg cggcattctc atctacaccg ggcagccgtg
    12001 gcaccctcaa ctggagatga ttgccggggt gttaaccagt cataaagatg gtaaaccgtg
    12061 ggtaatgcgc gtgcgttcgc aaggggagat ggattcactc gtgcgtgatg ccggatttga
    12121 taaatgcaca caacggattg atgagtgggg tatttttacg gtttcgatgg cggtgcgtcg
    12181 tgataactga acgtcggaac gtattgctac aaggcgctgg ctggttattg ttgctggccc
    12241 cgtttttctt cttcacctat ggatctctta atcagttcac cgcggttcag gaccttaaca
    12301 gccatgatat ccccagtcag gtattcggtt gggaaacggc gatccctttt cttccctgga
    12361 ctattgttcc ttactggagt ctggatcttt tatatggatt ttcgctgttc gtttgtagca
    12421 cgacattcga acagcgccga cttgtccacc ggcttattct ggcaacggta atggcctgct
    12481 gcggtttttt gctctatccg ctgaagttta gttttatccg tcctgaagtg agtggggtga
    12541 cgggatggct attttcgcaa cttgaactgt ttgatctgcc ttataaccag tctccttcgc
    12601 tgcatattat tctctgctgg ctactttggc gtcactttcg tcagcatctg gctgagaggt
    12661 ggcgtaaagt ctgcggcgga tggtttttac tcatcgctat ttctactctg acgacctggc
    12721 agcatcattt tattgatgtc atcacagggc tggcggtagg tatgttgatt gactggatgg
    12781 tgcccgtcga ccgtcgttgg aattatcaga aacctgatca acgtcgaatc aaaatagcac
    12841 tgccctatgt cgtaggcgcg ggctcgtgca ttgtattgat ggagctaatg atgatgattc
    12901 agttatggtg gtcagtctgg ttatgttggc cagtattatc gctactcata attggccgtg
    12961 ggtacggtgg gcttggcgcg ataacaacag ggaaagatag tcaggggaaa ctaccgcccg
    13021 ccgtttactg gctgacattg ccctgccgca tcgggatgtg gctgtctatg cgttggtttt
    13081 gtcgtcgcct ggagccggtg agcaaaatga ctgctggtgt ttatttaggg gcgtttccac
    13141 gacatattcc ggcacagaat gcggttctgg acgtcacctt tgaattccct cgcggacgag
    13201 cgacaaaaga tcgactctat ttctgtgtac cgatgctgga tctggtggtt ccggaagagg
    13261 gggagctccg acaggccgtg gcgatgctgg aaacattacg cgaagagcaa ggcagcgttc
    13321 tggtccattg cgcgttggga ttatcgcgca gtgcgctggt agtggcggca tggttgttat
    13381 gttacggaca ctgtaaaacc gttgatgaag cgattagtta tattcgagcc agatgttcgc
    13441 atattgtgct taaggaagat cacaaagcga tgctgaaatt atgggaaaac aggtaagtgg
    13501 attgagatgt ggactgaact acagtccaca tcaagatcgt gtccggttat gcagcaacaa
    13561 tgctgtcgat ggccgctttc gcgtcagact gcgctttcgc tgccatttcc ggaccgtatg
    13621 cgatcccttc ggcgaagaca aatttcacat cggtaatgcc gataaagccg aggaacgtgg
    13681 acagatacgg cgtcaccagg tccgttggtc catctttgtg gatcccaccg cggctggtaa
    13741 taacgatggc ttttttaccc gttaccagac cttccggacc gttctcggta tagcggaaag
    13801 taacgcctgc gcgtgcaacc aggtcaaaat agtttttcaa ctgagttgag atgttgaagt
    13861 tatacatcgg tgccgcaata acgataacgt cgtgggcttt cagctcggca atcagctcat
    13921 cggaaagcgc cagagcttcc tgctgacgcg gagtcagcgg cgcatcgctc ggacgcagtg
    13981 caccaaccag ttcgccatcc agtaccggaa tcggatttgc agccaggtcg cgaacggtga
    14041 tttcatcagc ggagtgcttt tcgcgccatt gttcaacaaa ataatcggac aactgattag
    14101 actgagagta ccctgccagg atgctggatt taagaactaa taccttgctc atggtgtttc
    14161 cttatagatg tttgaatggg cgatgccccg ttgcttgctg acactttatt cacaatcctg
    14221 ccacagagat agcgcaataa atcgaagcct atgttcgaat ttattgaaca acgcatagaa
    14281 agccgcgatg tggtactcta tatctatcat ttaaaagaaa attaatcagg cagactactg
    14341 cccactaacg ttatgacaga acaacaaaaa ttgaccttta cggccttgca gcagcggctg
    14401 gattcgctga tgctgcgcga cagactgcgt ttttctcggc gtctgcacgg tgttaagaag
    14461 gttaaaaatc ctgatgcaca acaggccatt ttccaggaga tggcgaaaga gattgaccag
    14521 gcggcaggga aagtcctgct gcgtgaagcg gcacgaccgg aaattactta tcctgacaat
    14581 ttaccggtta gtcagaaaaa acaggacatt ctcgaagcga ttcgtgatca ccaggtggtg
    14641 atcgtcgccg gggaaacggg ttctggtaaa acgactcagt taccgaaaat ctgtatggag
    14701 ctggggcgcg ggattaaagg gctgatcggc catacccagc cgcgtcgcct ggcggcaaga
    14761 acggtggcga accgtattgc ggaagagctg aaaacggagc cgggcggttg catcggttac
    14821 aaagtgcgtt tcagcgatca cgttagtgat aacacgatgg tcaagctgat gaccgacggt
    14881 atcctgctgg cggagatcca gcaagaccgc ctgctgatgc agtacgacac catcattatt
    14941 gacgaagcac acgaacgcag cctgaatatc gattttctgc tcggctattt gaaagagttg
    15001 ctgccgcggc gtcctgacct aaaaatcatt atcacttccg cgactatcga cccggaacgc
    15061 ttttcgcgcc actttaataa tgcgccgatt atcgaagtct ccggtcggac ctatccggtg
    15121 gaagtgcgct atcgaccgat tgttgaagaa gccgacgaca ccgagcgcga tcagttgcag
    15181 gcgatttttg acgccgtaga cgaactgagt caggaaagcc ctggcgacat tctgatcttt
    15241 atgagtggtg agcgggaaat tcgcgatacc gccgatgcgc tgaacaagct aaatctgcgt
    15301 cataccgaaa tcttgccgct ttatgcgcgg ctttcgaaca gcgaacagaa ccgcgtgttc
    15361 cagtcgcaca gcggacggcg cattgtgctg gcgaccaacg tcgcggaaac ctcgctgacc
    15421 gtaccgggga ttaaatacgt tatcgacccc ggtacagcac gtatcagccg ctacagctat
    15481 cgcaccaaag tgcagcgttt gccgattgaa cccatttcgc aggcgtctgc caatcagcgt
    15541 aaaggccgct gtgggcgtgt gtccgaaggg atctgtattc gtctctattc cgaagacgat
    15601 ttcctttcgc gcccggagtt taccgatccg gagattctgc gtaccaacct ggcctcggtt
    15661 attttgcaga tgaccgcgct ggggctgggc gatatcgctg cgttcccgtt tgtcgaagca
    15721 ccggataaac gcaatatcca ggatggcgtg cgtctgctcg aagaactggg cgcgatcacc
    15781 acggatgaac aggccagcgc ctataaactg acgccgctcg gtcgccagct ctcgcagttg
    15841 cctgtcgatc cacgtctggc gcgtatggtg ctggaagcgc aaaaacatgg ctgcgtgcgt
    15901 gaggcgatga ttatcacgtc cgcgctttcc attcaggatc cgcgcgagcg tccgatggat
    15961 aaacagcagg catcggacga aaaacatcgt cgcttccacg acaaagagtc tgacttcctc
    16021 gcgtttgtga atctgtggaa ttatcttggc gagcagcaaa aggcgctttc ttccaacgcc
    16081 ttccgtcgcc tgtgtcgtac cgattatctc aactatctgc gcgtgcgcga atggcaggat
    16141 atctacaccc agttgcgcca ggtggtgaaa gaacttggca ttccggttaa cagcgaaccg
    16201 gcggagtatc gcgaaattca cattgcgttg ctgacgggtt tactttccca tatcggcatg
    16261 aaagatgccg ataaacaaga atataccggc gcacgtaacg cgcgtttctc catctttccc
    16321 ggttctggtt tattcaaaaa accgccgaaa tgggtaatgg tggcagaact ggtagaaacc
    16381 agccgcctgt gggggcgcat tgctgcgcgt atcgacccgg aatgggtgga gcctgtcgct
    16441 cagcatttga ttaaacgcac ctacagcgaa cctcactggg aacgggcgca gggcgcggtg
    16501 atggcaacgg aaaaagtcac tgtttatggt ttgccgattg ttgccgcgcg caaggtcaac
    16561 tacagccaga tcgatccggc gttatgccgt gaactcttta ttcgccatgc gctggtggaa
    16621 ggtgactggc agacgcgtca cgcattcttc cgtgaaaacc tgaaactgcg ggccgaagtg
    16681 gaagagctgg aacacaaatc acgtcgccgc gatattctgg ttgatgacga aacgttgttt
    16741 gagttctatg accagcgcat cagccacgat gtgatctctg cacgccactt cgacagctgg
    16801 tggaaaaaag tcagccgcga aacccctgat ttgctcaact ttgaaaaaag catgttgatc
    16861 aaagagggcg cagaaaaaat cagcaagctg gattacccga acttctggca tcagggcaat
    16921 ctcaagctgc gtttgagcta tcagtttgag cccggcgcgg atgctgacgg tgtgaccgta
    16981 catattccgc tgccgttact taaccaggtt gaggaaagcg ggtttgaatg gcagatcccc
    17041 ggtctgcgcc gcgaactggt gattgctctg attaaatcgt tgccgaaacc ggtacgccgt
    17101 aattttgtac ccgcaccaaa ctacgccgaa gcgtttttag gccgcgtcac accgctggag
    17161 ttaccgttgc tcgacagcct tgagcgcgag ttacggcgga tgaccggtgt taccgttgac
    17221 cgcgaagact ggcactggga tcaggtgccc gatcacctga aaatcacctt ccgcgtggtg
    17281 gacgacaaaa ataagaagct aaaagaaggg cgctcattac aggatctgaa agatgcgctg
    17341 aaaggcaaag tgcaggaaac gctgtccgcg gtggcggatg acggtatcga gcagagcggg
    17401 ctgcatatct ggagctttgg gcaactgccg gaaagctacg aacagaagcg cggcaactat
    17461 aaagtgaagg cgtggccggc gctggtggat gaacgtgaca gtgtggcgat caaactgttt
    17521 gataacccgc tggagcaaaa acaggcaatg tggaacggtc ttcgccgtct actgctgcta
    17581 aatattccct cgccgatcaa gtatttacat gagaagttac caaacaaagc caagcttgga
    17641 ctgtacttta acccgtatgg caaagtgctg gagctgatcg acgactgtat ctcctgcggt
    17701 gtggatcaac tgatcgacgc caatggtggc ccggtctgga cggaagaagg ctttgctgcg
    17761 ctgcatgaga aagtgcgtgc cgaactgaac gacacggtgg tagatattgc gaagcaggtc
    17821 gagcaaattc ttacggcagt gttcaatatc aacaaacgtc tgaaagggcg ggtggatatg
    17881 accatggcac tggggctttc tgacattaaa gcgcagatgg gcgggttggt atatcgcggt
    17941 tttgtcactg gtaacggctt caaacggctg ggcgacacgc tgcgatattt gcaggcgatt
    18001 gaaaaacggc tggaaaaact ggcggttgat ccacatcgcg accgtgctca gatgctgaaa
    18061 gtcgaaaacg tccagcaggc gtggcagcaa tggatcaaca aactgccgcc tgcacgtcgt
    18121 gaggatgaag acgtgaaaga gatccgttgg atgatagaag agttgcgcgt tagttacttc
    18181 gctcaacaac ttggtacgcc ttatccgatt tcagataagc gtattttgca ggcgatggag
    18241 cagattagcg gttaaccctg ctatttgcct gataaagaaa aaacccggta agcatttagc
    18301 gccgggtttt ttattaattc taaaacggta agggtaaaaa ttcaggaatt cagaaaaata
    18361 caattctctg ctgcaagatg aataatgttt atctacagca tttccttaaa agatatgtca
    18421 ggcttgcgga gtggcggtta aggacatacg acttcctcct ttcagagtgc tccgcttctc
    18481 actattatct cacacagtat tcttaaggga acgataagga ggaaccatga acattacccc
    18541 gtttccgacg ctttcgccgg caactataga tgccataaat gttatcggac agtggctggc
    18601 gcaggatgat ttctccggtg aggtgccgta tcaggccgat tgcgtgatcc ttgcaggcaa
    18661 tgcggttatg ccgactatcg acgcggcatg taagattgct cgcgatcagc aaattccttt
    18721 actgattagt ggtggtatcg gtcactcgac aacttttttg tatagcgcca tcgcacagca
    18781 tccgcactac aacactatcc gcaccactgg cagagcagaa gcgaccatcc tggcggatat
    18841 cgctcatcag ttctggcaca ttccgcatga aaaaatctgg attgaagacc agtcaacaaa
    18901 ctgcggtgaa aacgcacgct ttagcatcgc gctattgaaa caggccgtag aacgagttca
    18961 tacggctatc gttgttcagg accccaccat gcagcggcgc acgatggcga cgttccgccg
    19021 tatgactggg gacaatcccg atgcaccacg ctggttaagt tatcccggat tcgttcctca
    19081 gttaggaaat aacgcagaca gtgtaatctt tattaatcag ttacaaggat tatggccggt
    19141 tgagcgttat ctctcactac tcactggcga gctgccgcgt ttacgcgatg atagcgatgg
    19201 ctacggtccc cgcgggcgag attttatcgt tcacgttgat tttccggcag aagtcatcca
    19261 tgcatggcaa acgctgaaac atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt
    19321 acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt
    19381 tatttctgcc ttttattcct tttacacttg tttttatgaa gcccttcaca gaattgtcct
    19441 ttcacgatgc cgtctctctg atgattgatg ttaattaaca atgcattcac cgaaaataaa
    19501 catataagtc acaggagtcg cccatgtcag tacccgttca acatcctatg tatatcgatg
    19561 gacagtttgt tacctggcgt ggagacgcat ggattgatgt ggtaaaccct gctacagagg
    19621 ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag
    19681 cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat tgaacgcgcc agttggttgc
    19741 gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag
    19801 aagggggcaa gatccagcag ctggctgaag tcgaagtggc ttttactgcc gactatatcg
    19861 attacatggc ggagtgggca cggcgttacg agggcgagat tattcaaagc gatcgtccag
    19921 gagaaaatat tcttttgttt aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga
    19981 acttcccgtt cttcctcatt gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca
    20041 tcgtcattaa acctagtgaa tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg
    20101 atgaaatagg ccttccgcgc ggcgtgttta accttgtact ggggcgtggt gaaaccgttg
    20161 ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag tatgacaggc agcgtctctg
    20221 caggtgagaa gatcatggcg actgcggcga aaaacatcac caaagtgtgt ctggaattgg
    20281 ggggtaaagc accagctatc gtaatggacg atgccgatct tgaactggca gtcaaagcca
    20341 tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg
    20401 tacagaaagg catttatgat cagttcgtca atcggctggg tgaagcgatg caggcggttc
    20461 aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg
    20521 cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt
    20581 tgggtggcaa agcggtagag gggaaaggat attattatcc gccgacattg ctgctggatg
    20641 ttcgccagga aatgtcgatt atgcatgagg aaacctttgg cccggttctg ccggtggtcg
    20701 catttgacac gctggaagag gctatctcaa tggctaatga cagtgattac ggcctgacct
    20761 catcaatcta tacccaaaat ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg
    20821 gtgaaactta catcaaccgt gaaaacttcg aagctatgca aggcttccac gccggatggc
    20881 gtaaatccgg tattggcggc gcagatggta aacatggcct gcatgaatat ctgcagaccc
    20941 aggtggttta tttacagtcg taatgagtaa aagaggcgga ggttttttcc tccgcctgtg
    21001 cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt
    21061 cgttatcgta ccaggcgacc gttttcacca gttgtaaatc gcccacggcg gtaatttccg
    21121 tttgcgtggc atcaaacacc gaaccgaaat ggctgccaat gatatcggaa gagactattt
    21181 cttcatcggt ataaccaaat gactcgttat tggtggttgc ttgtttaagt gcgttattca
    21241 cctcttcggc agtcactttt tttccgagaa tcgataccag ttcagtgacc gaacctgttt
    21301 tcaccggcac gcgttgcgca tgacctttca gtttgccgct cagttccggg atcaccagac
    21361 caatggcttt tgccgccccc gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag
    21421 cacgtaaatc tttaccacgt gggccatcca ccagtgactg ggtgccagta taggcatgaa
    21481 tggtcgtcat cgtgccgact tctatcccga aactgtcatg caaggctttg gccatcggcg
    21541 caagacagtt agtggtgcat gacgccacgg aaacaatggt gtcgttgcca tccagagtgt
    21601 cgtcattgac gttataaacg atagttttca tttcaccggc aggggcggaa atcaacacct
    21661 tcttcgcacc agcatcaaga tgcgcctgcg atttctcggc ggaggtataa aagccagtac
    21721 attcgacaat gatttctgcc cctttcgctt tccacggaat atttttagcc tctttttcgg
    21781 cgtaaaccgc gatacttttc ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc
    21841 aggggaacgg accatagttt gaatcatgtt tcagcagata ggcgagaatt tttggggaag
    21901 tgagatcatt aatagcgaca acgtctatgt tgcttttgac ttcaagtaat cgacgcaaca
    21961 ccagtcgacc gatacgacca aaaccgttaa taccaacttt actcatggtt ttctcctgtc
    22021 aggaacgttc ggatgaaaat tgatcctttc caggcttagc ccaggattgt gggatgggca
    22081 atctccattc ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa
    22141 ttttgagaaa agcccgttat gttaacggaa aattatgtta aagcaggaaa tgttatggaa
    22201 aataaatact caaggttaca aatcagcatt cactggctgg tctttttgct ggttatcgca
    22261 gcgtattgcg caatggagtt tcgtggtttc ttcccacgta gcgatcggcc actcatcaac
    22321 atggttcatg tttcctgtgg tatctcaatt ctcgtgctga tggtggttcg tcttttgtta
    22381 agactgaaat acccaacccc gccgattata cctaagccaa aaccgatgat gacgggactg
    22441 gcgcatttgg gacatttggt gatttatctc ctgtttattg cgctgccagt gattggtttg
    22501 gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg gtttgacgat gccttacgct
    22561 tcagaggcca atttcgaacg ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat
    22621 ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac tggcacacca ctatttctgg
    22681 aaggacaaca cacttctacg catgatgccg cgtaaacgtt cctgaaggat atttaaagaa
    22741 aacgcctgta ctaaaaccga cccgtggtac aggcgaagaa tacgggtcta catcggaagc
    22801 gcctattata tttatttgta tgacaaataa aaccataatc cttgcccata cgtccatctg
    22861 gcttattttt aatcaattca cccgatcttt gatctcatca acggtatcaa aataaaccag
    22921 cgtataacgt tcaaatcgaa cataagattc gataaaccat ggaggttata tgaaaaaact
    22981 ggcacttatt ttgtttatgg gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc
    23041 ctgttctggt tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa
    23101 tgatggttct attagtgcct cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc
    23161 atgagaaaat gaatctgcta gtcagatgcg cggggaaaat ccccgcgctt gcccttacct
    23221 ggacgtgcag gccatgagcg cagcaacctc cttatcaccg tcccggaacc gcagttcgta
    23281 gagcgtttgt cgggtcagga gtgtgaatgt cagaatcgta atgcagataa tgagcagaca
    23341 caccaccaga gggttgtgct tcatagcctt tctccttgcc ggatggcggg taagaggcta
    23401 agatctgaat tgctaggttc attcgttggc ctcggttgat agaaatatcg gtcggggcct
    23461 tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc
    23521 tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct gttttccaga
    23581 atttttcgtc attcgggtta gccagtttag ccattcgtta ctctctttat tccaatagca
    23641 ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt tggtcataag
    23701 taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga gagacaacgg
    23761 taatgaatac aactccctca catcgattag gttttttgca tcacatcagg ctggttccgt
    23821 tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct gctctggctg
    23881 gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag attgagattc
    23941 ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat atgattcagg
    24001 ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgcaatatt gcgcaagccg
    24061 aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga ccggtgaaaa
    24121 cacctgctga tgaagccctc gacactgaat taaatcaacg ctttcaggct tatatcacgg
    24181 gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt attaatcatg
    24241 aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac aaagccgtta
    24301 agatacgtag caccagggcc aaccaactgg cggaactggc ccatcagcgc acccgcctgg
    24361 gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg ataacattta
    24421 tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa
    24481 tcgctagtgg cgatctgacg atgaaggatg aaccggcggg tcgtaatgaa atcggtcgct
    24541 taaatcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg actgttcgac
    24601 agggtgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat gcggacctgt
    24661 catctcgcac cgaagaacaa gcggcggcaa tcgaacaaac tgccgccagc atggagcaac
    24721 tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa ctggcgcaag
    24781 aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta aaaacgatgg
    24841 gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc aacagtattg
    24901 ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga gcgggtgagc
    24961 aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc cgcagcgctc
    25021 aggcggcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt gacctggggt
    25081 cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg
    25141 tcacgcatat catgcaggaa atcgccgccg cctcggatga acaaagtaga ggcataactc
    25201 aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct tctctggtag
    25261 aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta actgaggcgg
    25321 tggatgtatt ccgtctgcac aaacattctg tgtcggcaga acctcgcgga gcgggtgaac
    25381 cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc ccttcacctt
    25441 tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag aagagaacgt
    25501 cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag
    25561 ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga caaaccacac
    25621 gtaatcgtat tcaaccgtaa gttgacgaga tagcgaagca gacagcgttt cgatacaacc
    25681 cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc gtggcgcagt
    25741 gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta cgttctcctg
    25801 aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca gcagttcgta
    25861 attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat cgatttcccc
    25921 ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca aggtcgtctc
    25981 tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg ccgcagtagg
    26041 tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa gcgactgtcc
    26101 ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa
    26161 ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgccagtca gctgctccag
    26221 ttcattcaat gtcttagaga gcgcaggttg actcaaatta agggtttcag ccgcgcgccc
    26281 caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg
    26341 actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt cgctgacaag
    26401 tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta aagcaattat
    26461 gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt gtaaattctt
    26521 cccgcaagag tgaatgcggt tacctacact ccagattact gaccactgga ggcagacact
    26581 atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc
    26641 atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc tgatgtgaat
    26701 ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg
    26761 gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca agagcttgct
    26821 actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta cgatctctcg
    26881 caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc ttctctggcg
    26941 cgtaatccct tccgcgtttt tacctcctta ctccgccttg agcttatcga gaacgaaatt
    27001 ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc acgttgtcga
    27061 caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc acaggagttt
    27121 gtgcaggaag ccctggaaac gtttcgctgg caccagtcag caacggtaga tgaagaaacc
    27181 tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt tcctggatgc
    27241 catatcaacc acctgacacc acgtacgctg gatattgacc gggtgcagtc gatgatgcct
    27301 gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga ggtaccgatt
    27361 ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc ggggcagaaa
    27421 cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc attaacgccg
    27481 aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca ggataatctc
    27541 actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga gtttttaatg
    27601 cgtcagcaag ggctggcatg gttccggtac cgtctgacgc cttcaggtga ggcgcatcgt
    27661 caggcgattc atcccggaga cgatccacag cccttaattg aacgtggttg ggtcgcggcg
    27721 caacccatta cctatgaaga tttcttgccc gttagcgcgg cggggatctt ccagtcaaat
    27781 ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc atttgagcag
    27841 gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga agaacgcagt
    27901 aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa tttgcctgat
    27961 tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt atgcatattc
    28021 tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt cataaataag
    28081 cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag aaggactcac
    28141 tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc gccgtgtgcg
    28201 gtaccagcgg cattgcttct cttttttctc aggcggcatt cgcggcagat tctgatattg
    28261 ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg cacgacttag
    28321 cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg acaatgacgc
    28381 cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat aacgttgaga
    28441 accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc cgcgttcgta
    28501 tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc ccggagttgt
    28561 tcaaatacaa cgatgcgggt gttgatacaa aacaattaga agggcaaagc gatctcggct
    28621 ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta gtatcatttc
    28681 tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg tcggcccgcg
    28741 gcctggcgat cgacacttac actgacagta aagaagagtt ccccgacttt accgccttct
    28801 ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg
    28861 ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag gtgattatgg
    28921 atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt gcgccgatga
    28981 ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt catccgcaaa
    29041 ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc
    29101 tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg aaagggtttg
    29161 gtttattgca actggatcgt gacttctccc attatcagga cattatgggc tggtataaca
    29221 aacgcccaag tctgtgggtg gagccgcgta acaagtgggg caagggcacc atcggcctga
    29281 tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg cagccagaaa
    29341 aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg agtgcgcaac
    29401 cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc
    29461 cggaaggttg ggcaccaggt gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg
    29521 atttcgttgg tggtgatctg aaagctgccg caccaaaagg cattgagccg gtgattacgc
    29581 tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatt gatggttatc
    29641 gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat atgcggatgt
    29701 atctgcgttg tcagggcgac gctatcagtg aaacatggct gtatcagtat ttcccgccag
    29761 cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt tttcttcggc
    29821 accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt tccctaagtt
    29881 aaatgagtaa tctgatggtg tgtatttcag atacaccttg tcagccacta acagggagtg
    29941 cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa tcctcgcttt
    30001 atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa ggaaggttcc
    30061 gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa
    30121 gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac tttcctaagc
    30181 cgccagccag gcggcttttt taacaacagc atggattgac tggagataag atgactgaaa
    30241 cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac gtcacacctc
    30301 tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac tggccgcagt
    30361 tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac
    30421 gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc gttatctcgt
    30481 ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg gacgaatctc
    30541 atcaggggca ggggatcatt tctcaggcgc tgcaggcatt gattcatcat tacgctcagt
    30601 ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa agcaaccagg
    30661 tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag ttcctgaatg
    30721 atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa ccctgccagc
    30781 ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac atgaaccgtg
    30841 tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt ttcaattatc
    30901 accgcacctg aaatacggct ctcaccttgt attacaacgt gctcgtcgag cagaatcggt
    30961 ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac acaattacct
    31021 tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat ggcatcttct
    31081 tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca tagccagacg
    31141 ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg atgttcaata
    31201 aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat acgcgaggcg
    31261 ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt taagccttgt
    31321 gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct acattgcccg
    31381 taaactaatg agtcatgaat ggtgacactg tcacttatgt atgcaccctg gctgatttca
    31441 ctattatcga tccagacatt atccgttgcg taaacctctc cccacagtac gctggtgccg
    31501 gtgatgcgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa
    31561 atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc gccagcggtt
    31621 cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa cacatttttt
    31681 ttagtgccat cttcctgata actaaaggct cgctgttctt cacttagacg atatttgcgc
    31741 atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc tctggctatg
    31801 ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac aaaatgagta
    31861 agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt attgtgttgg
    31921 ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag gtcagccact
    31981 ggttagggga tgggctggtg attctggcga tgatcatctg gggattatta actagcgcat
    32041 ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc catccagtgc
    32101 tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt ggttttgttc
    32161 cgtggtttcg cccactggcg gtgtgcctgt tcagttttgg tgtcgtggtt cagttggctt
    32221 atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa gctaccacgc
    32281 ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc tgtggtgcgt
    32341 tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca tggctaagcc
    32401 ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg gcactgcgga
    32461 catcactcgg cattcagctc gctcctgcgc tggtggcttg tagtgcctgg ctgagcgtca
    32521 acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg ctgcaactgc
    32581 tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct tcattctgga
    32641 gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg agtggcagcg
    32701 ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat tttattattg
    32761 caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta gtcagaaccg
    32821 agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg aaaactattt
    32881 tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg tgaaagtggt
    32941 taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc tttacctggc
    33001 agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg ccaacgtcga
    33061 gcgcattaaa tccattgaaa atctggataa tttacacacc cgagtcgttg atctgaataa
    33121 cctcacattt gatagacagt acgattttat tctttcgact gtggtactga tgttccttga
    33181 ggctaaaacc atcccagggc tgattgtcaa tatgcaacgt tgcactaaac ctggtggtta
    33241 caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg gcttcccgtt
    33301 tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg tgaaatacaa
    33361 tgaagacgtc ggcgaactgc accgcaccga cgccaacggt aatcgtatta aactgcgttt
    33421 cgccacgatg ctggcacgta aaaaatgacc cgctaagaat aaggcgcgtg aaaattcccc
    33481 acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg aagggatgtg
    33541 aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt taactactga
    33601 tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg ctgaaaaatt
    33661 tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct ataacttaga
    33721 aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc gaaagttgcg
    33781 gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca gccagataaa
    33841 tattctggtt ttttaaacaa ttactctgat ttaaaagaaa caacctcagc aacaggtaaa
    33901 cctgttttac gttgggtaga cccgagtttt gatcaaagca aatatgacag catcgtctgg
    33961 aacccaatca cttattatcc ggtaccaaaa ccttctaccc aggtagggca gaaagttctg
    34021 gataaaattt tgaactatac caacaccgaa atgaaagaag cgatagcgca gcgtaaacca
    34081 ctggttacca ccgctgggcc gcgtagtctg attttccgtg gggccattac cggtgtagat
    34141 accagcaaag aagggctgca attctatgaa gtggttcctg ttgcattagt ggttgcgggg
    34201 acacaaatgg ctacaggcca ccgtaccatg gatactcgcc tctattttga aggtgagctg
    34261 attgatgcag cgactaataa accggttatc aaagtcgttc gtcagggcga aggtaaagac
    34321 ctgaataacg aaagtacgcc aatggctttc gaaaatatta aacaagttat tgatgacatg
    34381 gcgaccgatg ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg ccatcgttag
    34441 atggcgcttt ttatccggtg cgccgtaaaa ccccatcctt cagggcgggg atataaggcg
    34501 cggttttcca cctgaccagg tgtttgctgt tgttcaatgt attggcggat gacggatatt
    34561 ggcgcacctc cgcagctact ggcaaaatag ccaggactcc acaaaacgcc tttgtagtaa
    34621 tacctgactg caatatctgg tcgatctcgt cgcagtaacc tactcgatac gcctttgagg
    34681 ctgtttacca gactggatat cgccagtttg ggaggatagt taattaacaa atggacgtga
    34741 tctggttcgc catccattta aaccagttca gcttcaaaat cagcacatac atttgaaaag
    34801 taagtgcgta gtttttctgt cgcgtcatag tcaaaaatct gacgtcggta tctggtgaca
    34861 aagaccaggt gaacatgcat caggaaaaca caatgcttcc ccgtcggata tcggtttctt
    34921 tattcataga ccaaagtatc atttggcctg tgaaacgatt acaggcattt aaattccagt
    34981 taagacccgg tggtcaacag gagcgtgaaa tgaggcgctt cgcaggcgca tgtcgtttcg
    35041 ttttcaatcg tgctctggcg cttcagaatg agaatcatga ggccggtaat aaatacatcc
    35101 cttacgggaa aatggcttcc tggctggttg agtggaaaaa cgccactgaa acgcaatggc
    35161 ttaaagattc gccctcacag ccattgcaac agtcactgaa aggccttgag cgggcttaca
    35221 aaaacttctt ccagaagcgg gctgcttttc cccgattcaa aaagcgggga cagaatgatg
    35281 cattccgcta cccgcagggc gttaagctcg atcaggaaaa cagccgtatt tttctgccga
    35341 aactggg
//
LOCUS       DAHBHC010000052        35321 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHBHC010000052 DAHBHC010000000
VERSION     DAHBHC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.052"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(20..385)
                     /gene="mdtJ"
                     /locus_tag="M2A35_002986"
     CDS             complement(20..385)
                     /gene="mdtJ"
                     /locus_tag="M2A35_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCB7199254.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            797..1831
                     /gene="tqsA"
                     /locus_tag="M2A35_002987"
     CDS             797..1831
                     /gene="tqsA"
                     /locus_tag="M2A35_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCB7199255.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFAFNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(1856..3244)
                     /gene="pntB"
                     /locus_tag="M2A35_002988"
     CDS             complement(1856..3244)
                     /gene="pntB"
                     /locus_tag="M2A35_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCB7199256.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3255..4787)
                     /gene="pntA"
                     /locus_tag="M2A35_002989"
     CDS             complement(3255..4787)
                     /gene="pntA"
                     /locus_tag="M2A35_002989"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCB7199257.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKTAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5311..6255
                     /locus_tag="M2A35_002990"
     CDS             5311..6255
                     /locus_tag="M2A35_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7199258.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            6441..7823
                     /locus_tag="M2A35_002991"
     CDS             6441..7823
                     /locus_tag="M2A35_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCB7199259.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            7860..8582
                     /gene="folM"
                     /locus_tag="M2A35_002992"
     CDS             7860..8582
                     /gene="folM"
                     /locus_tag="M2A35_002992"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCB7199260.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(8579..8914)
                     /locus_tag="M2A35_002993"
     CDS             complement(8579..8914)
                     /locus_tag="M2A35_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCB7199261.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9043..9762
                     /gene="rstA"
                     /locus_tag="M2A35_002994"
     CDS             9043..9762
                     /gene="rstA"
                     /locus_tag="M2A35_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCB7199262.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            9766..11067
                     /gene="rstB"
                     /locus_tag="M2A35_002995"
     CDS             9766..11067
                     /gene="rstB"
                     /locus_tag="M2A35_002995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCB7199263.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11143..12072
                     /gene="tus"
                     /locus_tag="M2A35_002996"
     CDS             11143..12072
                     /gene="tus"
                     /locus_tag="M2A35_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCB7199264.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12069..13472)
                     /gene="fumC"
                     /locus_tag="M2A35_002997"
     CDS             complement(12069..13472)
                     /gene="fumC"
                     /locus_tag="M2A35_002997"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCB7199265.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(13615..15261)
                     /gene="fumB"
                     /locus_tag="M2A35_002998"
     CDS             complement(13615..15261)
                     /gene="fumB"
                     /locus_tag="M2A35_002998"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCB7199266.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            15460..16635
                     /gene="manA"
                     /locus_tag="M2A35_002999"
     CDS             15460..16635
                     /gene="manA"
                     /locus_tag="M2A35_002999"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCB7199267.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            16736..18244
                     /locus_tag="M2A35_003000"
     CDS             16736..18244
                     /locus_tag="M2A35_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCB7199268.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLESMEINGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMLALNVPVVPAIPQQ"
     gene            complement(18289..19554)
                     /gene="uidC"
                     /locus_tag="M2A35_003001"
     CDS             complement(18289..19554)
                     /gene="uidC"
                     /locus_tag="M2A35_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCB7199269.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(19593..20966)
                     /gene="uidB"
                     /locus_tag="M2A35_003002"
     CDS             complement(19593..20966)
                     /gene="uidB"
                     /locus_tag="M2A35_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCB7199270.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(20963..22774)
                     /gene="uidA"
                     /locus_tag="M2A35_003003"
     CDS             complement(20963..22774)
                     /gene="uidA"
                     /locus_tag="M2A35_003003"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCB7199271.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(23162..23752)
                     /gene="uidR"
                     /locus_tag="M2A35_003004"
     CDS             complement(23162..23752)
                     /gene="uidR"
                     /locus_tag="M2A35_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCB7199272.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(23981..24748)
                     /gene="hdhA"
                     /locus_tag="M2A35_003005"
     CDS             complement(23981..24748)
                     /gene="hdhA"
                     /locus_tag="M2A35_003005"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCB7199273.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVLSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(24860..25888)
                     /gene="malI"
                     /locus_tag="M2A35_003006"
     CDS             complement(24860..25888)
                     /gene="malI"
                     /locus_tag="M2A35_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCB7199274.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEEMHSRNLII
                     PARLIAAK"
     gene            26063..27655
                     /gene="malX"
                     /locus_tag="M2A35_003007"
     CDS             26063..27655
                     /gene="malX"
                     /locus_tag="M2A35_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCB7199275.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            27665..28837
                     /gene="malY"
                     /locus_tag="M2A35_003008"
     CDS             27665..28837
                     /gene="malY"
                     /locus_tag="M2A35_003008"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCB7199276.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            28941..29942
                     /gene="add"
                     /locus_tag="M2A35_003009"
     CDS             28941..29942
                     /gene="add"
                     /locus_tag="M2A35_003009"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCB7199277.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(29977..31017)
                     /locus_tag="M2A35_003010"
     CDS             complement(29977..31017)
                     /locus_tag="M2A35_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCB7199278.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            31260..31385
                     /gene="blr"
                     /locus_tag="M2A35_003011"
     CDS             31260..31385
                     /gene="blr"
                     /locus_tag="M2A35_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCB7199279.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            31658..31873
                     /gene="ydgT"
                     /locus_tag="M2A35_003012"
     CDS             31658..31873
                     /gene="ydgT"
                     /locus_tag="M2A35_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCB7199280.1"
                     /translation="MTVQDYLLTFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            31959..32399
                     /locus_tag="M2A35_003013"
     CDS             31959..32399
                     /locus_tag="M2A35_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCB7199281.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            32476..33057
                     /gene="rsxA"
                     /locus_tag="M2A35_003014"
     CDS             32476..33057
                     /gene="rsxA"
                     /locus_tag="M2A35_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCB7199282.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            33057..33635
                     /gene="rsxB"
                     /locus_tag="M2A35_003015"
     CDS             33057..33635
                     /gene="rsxB"
                     /locus_tag="M2A35_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCB7199283.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            33628..>35321
                     /gene="rsxC"
                     /locus_tag="M2A35_003016"
     CDS             33628..>35321
                     /gene="rsxC"
                     /locus_tag="M2A35_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCB7199284.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         8614 a         9145 c         8619 g         8943 t
ORIGIN      
        1 ccaggcggcg tgaacccatt caaactgcgc catggttcac ctccagttca ggtttacgcg
       61 ctttacgggt acctgatttt atcaacacaa tcccggcgac cagggtggtt aacccggcaa
      121 ttttcatcag cgataaactt tcgtcgaata acaaaacgct aaacaaggta ataaataaaa
      181 taccgatacc ttcccacagc gcataagcta cgcctaaggc gattttttta acggcgaaag
      241 agagaaatat atacgacaga gaaatcatca ccagcattaa aataaagccg ccatttccct
      301 cactgacgct cgcccatttc atggacagcg taccggtaat ttctgtagca atagccagac
      361 ctaataaaat ccaataaata tacattgtcc ttctcctgca agagaattat tttaattttc
      421 gcttaattca gcgaaaccaa agtaaagtgg tcaagccccg cgcatagggc aagcttaggc
      481 agaagaaagg actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat
      541 gaggtacgaa gatgggtaaa tgtagaaaat aacgtcctga acaaattgtc cataatatta
      601 caattatccg cagtgttgct tctcgtcatc gcggatgata attgtcctcg gtagttgaac
      661 acgcctgatt tgtatcatag cttaagaatt aactcaaaat attttcactt ctttacctga
      721 gcggtttgat tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga
      781 aaaaacgggg acaagcatgg caaagccgat catcacgctc aatggcctaa aaatcgtcat
      841 tatgttggga atgctggtca ttattctctg cggtatccgt tttgccgccg agatcatcgt
      901 gccgtttatt ctcgcattat ttattgctgt tattcttaac ccgctggtgc aacacatggt
      961 ccgctggcga gtgccgcgtg tactggcggt gtcgattttg atgaccatca tcgtgatggc
     1021 gatggtgttg ctattagctt atctgggttc cgcgctcaac gagttgacgc ggacgttacc
     1081 gcaatatcgc aactctatta tgacgccgct gcaagctctt gagccgttgt tgcaacgcgt
     1141 agggattgac gtctcagttg accagctggc gcattacatt gatccgaacg cggcgatgac
     1201 gttgctcacc aacttattga cgcagttatc taatgccatg tcatcaatat ttttattgct
     1261 gctgacggtg ctgtttatgc tgctcgaagt gccacaattg cccggaaaat ttcagcaaat
     1321 gatggcgcgt ccggttgaag ggatggcggc gattcaacgt gcaattgaca gtgtttctca
     1381 ttatctggtg ctgaaaacag ccatcagcat catcaccggc ctggtcgcct gggcgatgct
     1441 cgccgcactc gatgttcgct tcgcttttgt ctggggattg ctggcctttg cgtttaatta
     1501 catccctaat attggttcag ttctcgcggc aatcccccct atcgctcagg tactggtgtt
     1561 taatggcttc tacgaagcgt tgctggtgct ggcgggatat ctgctgatta atctggtctt
     1621 cggcaatatt ctggagccgc gcatcatggg acgtgggctg gggctttcca cattggtggt
     1681 atttttgtcg ttgatttttt ggggatggtt gttaggaccg gtgggtatgc tgctttccgt
     1741 gccgttaaca attattgtca aaattgcgct tgaacaaaca gcgggaggtc aaagcatcgc
     1801 cgttctgtta agcgatctca ataaagagtg acggcctccg cagaggccgt cagggttaca
     1861 gagctttcag gattgcatcc acgctggctt tggcgtcacc aaacagcatg tgggtgtttt
     1921 ccttgaagaa cagcgggttt tgcacaccag catagccagt gttcatcgaa cgtttaaaga
     1981 caatcacgtt ctgcgctttc cacacttcca gcacaggcat accagcaatc ggactcttcg
     2041 gatcatcctg cgccgccggg ttaaccgtat cgttagcacc aatcaccagt acggtatcgg
     2101 tatcagcaaa gtcatcgttg atctcgtcca tttccagcac gatgtcatac ggtacttttg
     2161 cttcagccag caatacgttc atatgtccag gcaaacgccc cgcaaccggg tggataccga
     2221 aacgcacgtt gataccacga gcgcggagtt tctcggtaat ttcagcgaca ggatattgcg
     2281 cctgcgcgac tgccatgccg taccccggag taatgatcac tgaatgggag tttttcagca
     2341 gttccgctgt ctcttctgcg gtgatttcgc ggtgctcacc cacttcctga tcatcgccag
     2401 tagaagagcc gtcggtgccg aaaccacccg caataacgct gataaaggaa cggttcatcg
     2461 ccttacacat aatgtaagaa aggatagccc ccgaagaacc gaccagcgca ccggtcacaa
     2521 tcagcaggtc gttgctgagc ataaagcccg cagccgcagc cgcccagccg gagtacgagt
     2581 tcagcatcga caccaccact ggcatatctg caccaccgat ggaggcgact aaatgccagc
     2641 cgaataccag cgcaatggcg gtcattatca gcaatgccag cacttgcagg ccgacgctgt
     2701 ccgtgcgaac aaatacaatc agcagcagga aggaaacgac cagagccgcc aggttcattt
     2761 tgtgacggtt tggcagcatc aacggtttag acgaaatctt gccacacagt ttgccgaacg
     2821 ccaccaccga acccgtgaac gttaccgccc cgatgaagat accgaggaac acttccgtca
     2881 ggtgaatatt gaccagaatc ggtgccattc ccgcgtcatg atgcagataa ctgttaaagc
     2941 caaccagcac tgccgccaga cccacgaagc tatgcaggat cgccaccagt tctggcattt
     3001 cggtcatttc aactttcttc gccagacgga taccaattgc cccaccaatg accatcgcca
     3061 gcaagatcca gccaacatta cccgtatccg gtccaaaaat ggttgcgatt aacgcaatcg
     3121 ccatcccggc gataccgaag ttgttaccct ggcgagacgt ttcatgtttc gaaagaccgg
     3181 ccagactgaa gataaacagg atcgcggcaa caatgtatgc agctgtaact aatcctccag
     3241 acatatgtta ccccttaatt tttgcggaac attttcagca tgcgctgagt cacggtgaag
     3301 ccaccgaaaa tattaatgct ggctataagc accgcgataa aactaaggaa gctaacccag
     3361 ccgccctggc caatctgcaa cagtgctccg acaacaataa tccctgaaat cgcgttggtg
     3421 accgacatca acggtgtatg cagcgcatgc gatacattcc acaccacgta ataaccgaca
     3481 acgcaggcca gcgcgaaaac ggtgaagtgc ccaagaaact ctttcggcgc aacgcttgcc
     3541 agccaaccga aaaggatgat tgccagcgcc atcaatgcgt atttacgcca cggtgagcag
     3601 gcacattttt cctcagtttt cacttccggt gccgcttttt gtgccgcctg cggctgagct
     3661 gatacctgaa tcggcggtgc cggccaggta atttcgcccg cacggatcac ggtcacgccg
     3721 cgaatcacca catcatcaaa atcaacagtg atattgccgt ctttctcttt gcacaacagt
     3781 ttcagcagat taacgaggtt agtaccgtaa agctgtgagg attgcgtcgg cagacggccc
     3841 ggaagatcgg tataaccaat cactttgaca ccattttccg tagtgaagat ttcacccggc
     3901 acggtgtatt cacagttgcc accgttttgg gctgccaggt cgacaatcac actgcccgcc
     3961 ttcatggagt caaccatttc acgggtaatt agcttcggcg ctgttttgcc tggaataagc
     4021 gcggtggtga caatgatatc gacctctttt gcctgggcgg caaagagttc catttccgct
     4081 ttgatgaacg cgtccgacat cactttggca tagccatcgc cgctgcccgc ttcctcttta
     4141 aaatccagct cgaggaattc cgcgcccata ctttgaactt gttctttcac ttccgggcgg
     4201 gtgtcgaatg cacgcacaat cgcgccgaga ctgtttgctg cgccaatggc ggccagacct
     4261 gcaacacccg caccaatcac catcactttt gccggtggca ctttcccggc cgcagtaatt
     4321 tgcccggtaa agaagcgccc aaattcatgt gccgcttcaa caatggcgcg ataaccggcg
     4381 atgttcgcca tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg cggcacagaa
     4441 tccatcgcca tcacggtcac gttacgttcc gcaagttttt gcattaattc cggattctgc
     4501 gcaggccaga taaaactcac cagcgttgtc ccaggattca gtaacgcaat ttcatcatct
     4561 aacggcgcat tgaccttcag aatgatctct gactgccaga cgctattccc ttctacaatt
     4621 tcagcgcccg cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc cgcgccgctc
     4681 tctaccgcga cggtaaaacc cagtttcagc agctgttcca ctgtttttgg cgttgctgca
     4741 acacgggttt cattggttaa ccgttctctt ggtatgccaa ttcgcatgat attcccttcc
     4801 atcggtttta ttgatgatgg tttgcctgtg tcaggagcca cacaagccgc tcatgtacga
     4861 gctaaatgtt actccgttaa aataaattag taacaaacgc cctataacgt actgaaaatt
     4921 atgcctgtga tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa atattaacga
     4981 ttcagccgtg atagtgggat aaacacctta gaacgccgga taaagactga taattgtctt
     5041 cgacggtcgg gtaaaacgag acatcgcccc ggcacgaatc actacttaac attaaattaa
     5101 cttatataat tcagttgctt cagtagtaat gatgctgata cggctgtttt ttaagcatag
     5161 acggtcattt gagcaggatt aaaattggct taaagaatgt gatatgaaaa atgacgcaga
     5221 cagttacacc gtttaaatgc aataatcagc cacgtttctc gttaataaca ataccagtac
     5281 ctggtttgcg caaggcgaag gattattttt atgaagctta agaacaccct cctggcgtcg
     5341 gcactgcttt ctgctacggc attctccgtt aacgcagcaa cagaactgac accggagcaa
     5401 gcggcagcgg ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa tgctattggc
     5461 gaagcggtga aagccgtttc tcgtcgcgca gataaagaag gtgccgcctc tttttatgtt
     5521 gtcgacactt ctgattttgg taacagcggt aactggcgtg tggtcgctga cctctataaa
     5581 gccgatgctg aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt tgtcgaactg
     5641 ccgaaagatc aggctgttct gattgaaccg tttgacacgg tcaccgtcca gggcttctat
     5701 cgtagccagc cagaagtcaa tgatgccatc accaaagcgg caaaagcgaa aggtgcctac
     5761 tctttctaca tcgttcgtca aatcgatgcc aaccagggcg gcaaccagcg tattactgca
     5821 ttcatctata aaaaagatgc taagaaacgt atcgtccaga gcccggatgt gatcccggca
     5881 gattccgaag caggacgtgc agctctggct gccggtggcg aagccgcgaa gaaagttgag
     5941 atcccaggtg ttgcgactac cgcatcacca agttctgaag tcggtcgttt ctttgaaacc
     6001 cagtcatcaa aaggcgggcg ttacaccgtc acgctcccgg atggcactaa agtcgaagaa
     6061 ctgaacaaag cgaccgcagc gatgatggtc ccgttcgaca gcatcaaatt ctctggcaac
     6121 tacggcaaca tgaccgaagt ctcctatcag gttgcgaaac gtgccgcgaa gaaaggtgct
     6181 aagtattacc acatcacccg ccagtggcag gaacgtggta ataacctgac cgtcagcgca
     6241 gatctgtata aataactatt cagttactcc tataaggcgg cttgatgccg cctttcgcat
     6301 ttttagtcac aaatttcccc gcgaccattg catgcagaca catctcttcg taaaatcccg
     6361 cgcttcagtg gcaaactcca tttttatgcg ttttgcccta atatttattc ttcttatcac
     6421 gttttaatca ctggatatcg atggaaaaga aactgggact gagcgcactc accgcgctgg
     6481 tattaagctc aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg gcggcagttg
     6541 ccagcccggc agcactgctc atcggctggg gtattactgg cgctggcatt ttattgctgg
     6601 cctttgccat gctgatcctc acgcgcattc gtcccgaact tgacggcggt atctttacct
     6661 atgcccgcga agggtttggc gagctaatcg gcttttgttc cgcatgggga tactggctgt
     6721 gcgcagtcat cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta agctttttta
     6781 cggacacgcc ggaattgcgc ctgttcggcg atggtaatac ctggcaatcg atagtcggtg
     6841 catcggcttt attgtggatt gttcactttt tgatcctgcg cggggtgcaa accgctgcca
     6901 gcattaacct ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt gttgtgctgg
     6961 cgatgatgat gttcaaactg gataccttca agctcgactt caccggactt gcacttggcg
     7021 tacccgtgtg ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt ttcattggtg
     7081 tggaaggtgc cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt ggcaaagcga
     7141 cactgctggc ggttctctcc gctctgggcg tttacttgct ggtaacgctg ctttcgctgg
     7201 gcgtggtggc acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc ggtctgatgg
     7261 tggaaatgat gggaccatgg ggcgaaatca tcatcgctgc cggtttgatc gtttccgttt
     7321 gcggcgcgta cttaagctgg accatcatgg cggcagaagt tccgttcctg gctgccactc
     7381 ataaagcatt cccacgcatt tttgcgcgcc agaacgcaca agctgcgcca tccgcctcgc
     7441 tgtggttgac caacatctgc gtgcaaattt gtctggtact catctggctg accggttccg
     7501 attacaacac gttgctgacc atcgcctcag aaatgattct ggtgccctat ttcctggtcg
     7561 gcgcgttctt gctgaaaatc gcaacccgtc cactgcataa agcggtaggt gtcggtgcct
     7621 gcatttatgg cttatggtta ttgtacgcct ctggtccaat gcacttgctg ttatccgttg
     7681 ttctgtatgc gccggggctg ctggttttcc tgtatgcgcg caaaacgcat acacatgata
     7741 atgtactgaa ccgtcaggaa atggtgctga ttggcatgct gttaattgcc tcagttccgg
     7801 cgacctggat gctggtggga taacgttcat cccatcgttg gatagcaaag gagataacga
     7861 tgggtaaagc ccagcccttg ccaatattaa ttactggcgg aggtcgtcgc atcggcctcg
     7921 ccctcgcctg gcatttcatt aatcaaaagc aaccggtgat tgtcagctat cggacgcact
     7981 atccagccat tgatggactg attaaggcag gtgcgcagtg tattcaggct gatttttcga
     8041 ccaacgacgg tgtgatggcg tttgccgatg aagtactaaa aagcacccat ggtctgcgtg
     8101 ctattttgca taacgccagt gcgtggatgg cggaaaaacc aggtacacca ctggccgacg
     8161 tactggcttg catgatgcag atccacgtta ataccccata cctgctcaac catgcgctgg
     8221 aaagattact gcgtgggcac ggacacgccg ccagcgatat cattcacttt accgattatg
     8281 tggtggagcg cggtagcgac aaacatgttg cgtatgctgc aagcaaagcg gcactggata
     8341 atatgacccg ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat tctattgcgc
     8401 catcgctgat cctgtttaat gaacatgatg atgccgaata tcgacaacag gcgctgaata
     8461 aatcactgat gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc gattacttac
     8521 ttaccagctg ctttgtcacc ggacgcagtt tcccacttga tggcggtcgt catctgcgtt
     8581 aatgcagctt tatccagcat ataatcaaca cccatgcgct tatcccccaa cacgcaacag
     8641 aaccaacaaa agcggcgggc aagcgcatca ttccggtgaa ataccacagc gacaccaggt
     8701 aaacaaaata gggaataatc gaccacatac taaaaatgat ggttgcgcgt aaggcttcaa
     8761 tgccgcgttc gctggcaaca atataatgcg cgataagcgc aaaggtcggg aaaagtggaa
     8821 tcagcccggc gatataataa tttttcgttt ttgctaaaac accaatcaac agcactacca
     8881 gcgcaccgag cgcggctttg attaccagcc ccatcttttt accttaacac ttccataaca
     8941 agtcatcagt agaatacctg atgaaaactt gtttagaaac gattgatagt aagtaaaaac
     9001 agcgcggtgt attgtgacgt ttttatatct accgtgaatg ttatgaacac tatcgtattt
     9061 gtggaagatg atgcggaagt cggttcactg attgccgcgt acctggcaaa acatgatatg
     9121 caggttaccg tagaaccgcg cggcgaccag gccgaagaaa ccattttgcg agaaagtccg
     9181 gatttggtgt tactcgacat catgctacca ggcaaggacg gcatgaccat ttgtcgtgat
     9241 ttacgcgcaa agtggtctgg accgattgtt cttctaacct ctctcgatag cgatatgaac
     9301 cacatcctgg cactggaaat gggtgcctgc gactatattc tcaaaacgac gccccctgct
     9361 gttttgctag cgcgtttacg tttgcatttg cgtcagaatg agcaagccac actgaccaaa
     9421 ggtcttcagg aaacgtctct gactccctac aaagccctgc atttcggcac gttgaccatc
     9481 gatcccatca accgcgtagt caccctggct aacactgaaa tctcgctctc gacagctgat
     9541 ttcgaattat tgtgggaatt agctacccat gccgggcaaa tcatggaccg cgatgcattg
     9601 ctgaaaaatt tacgcggcgt cagttatgac ggactggatc gtagcgtgga cgtggctatt
     9661 tcgcggttaa gaaaaaaact gctcgataac gccgcagaac cttatcgcat taaaactgtg
     9721 cgtaacaaag gctatctttt tgcgcctcat gcatgggaat aagcgatgaa aaaactgttt
     9781 atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct ggttgggctg
     9841 gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt gatgaacagt
     9901 tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg gggtaagacg
     9961 ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact gagtaaatac
    10021 catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc cctggacgat
    10081 cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt tggtcctgtt
    10141 ccttatcttt attatctcca tcagatgcga ttgctggata tcgccctgat cacttttatt
    10201 gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca ggatatgtta
    10261 aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg tatccacttt
    10321 gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc ggacaatatc
    10381 aacgccttga ttgccagcaa aaaacagctt attgacggta tcgctcacga actgcgaaca
    10441 ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc cgccgaatcc
    10501 caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct gctgacttat
    10561 gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct gccgttgtgg
    10621 ctgtcaacgc atctggcaga cattcaggca gtaacgcccg ataaaacggt acggattaaa
    10681 acgctcgcgc aaggccatta tgcggcgttg gatatgcgct taatggagcg cgtgctggat
    10741 aatttgctca ataacgccct gcggtactgc cattcaacgg ttgaaaccag cctgctactg
    10801 tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc cccagaaaac
    10861 cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg ctcaaccggc
    10921 ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg cggtacggtt
    10981 aattgtgaca ccagcgaact gggtggtgct cgcttctcgt ttagctggcc gttatggcat
    11041 aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt caccaccact
    11101 gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg ttacgatctc
    11161 gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctgc atttgccgct
    11221 catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga ggtaaaaaaa
    11281 gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg caacgacgcg
    11341 cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc cgaaaatcgc
    11401 agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga taacctttcg
    11461 caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac gttcgagcat
    11521 atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt gcatcgtcat
    11581 ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca cgaccccgcc
    11641 actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg tgatgaagtc
    11701 ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg gacgcgcgag
    11761 gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc acagaacgcg
    11821 aagttaaaaa tcaaacgtcc ggtaaaggtg cagccgattg cccgcgtctg gtacaaagga
    11881 gatcaaaaac aagtgcaaca cgcctgccct acaccgctga ttgcactgat taatcgggat
    11941 aatggtgcgg gcgtgccaga cgttggtgag ttgttaaatt acgatgccga caatgtgcag
    12001 caccgttata aaccacaggc gcagccgctt cgtttgatca ttccacggct gcacctgtat
    12061 gttgcagatt aacgcccggc tttcatactg ccgaccatct gttctggccg tacccagctg
    12121 tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa ggtcagccct
    12181 tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg ggtgttaagc
    12241 gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg attcggttca
    12301 atacccactg cacagtgttt gttaaaactt tccatgccat ctgccagcaa gcgcaccgat
    12361 tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt accggaagcg
    12421 ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat ggttaatgct
    12481 tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc attttccggg
    12541 attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac atcattggcg
    12601 attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac cagggcatca
    12661 caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca ggtaatgact
    12721 gccagttcat ctgctacgcg acgcgcatac tccggatggg tatttagtcc agtacccacc
    12781 gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat atgtttgaga
    12841 ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag cggcgtggcg
    12901 tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga tttttcactc
    12961 agtgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagcgc cagcagcgcc
    13021 gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt cacgtcgtcg
    13081 ttagggtgaa ctttacgttc catcccgcgc acgccgccga gtaattcact ggcccggtta
    13141 gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg ccagatagcc
    13201 agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg ccgaatggcg
    13261 ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc tgcgcgcttg
    13321 gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat gcggaaatgc
    13381 tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc aatcgccccc
    13441 atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctgt ttaatttttt
    13501 ctttctgttt tgctttcgtt aagcaacttt tagttcactt attatttact atttgataac
    13561 aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct ctgtttattt
    13621 cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc ctttatcatc
    13681 cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt ccattcccag
    13741 ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata cagcggccgg
    13801 accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg tcacctgctg
    13861 gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca gttgatcgac
    13921 ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat aaccttccgg
    13981 cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtactgcg gcagcccttc
    14041 accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccga cgataatcgt
    14101 gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga ggatctcttt
    14161 catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac gcagctcttc
    14221 agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct gacggttgat
    14281 cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg gacaggatgc
    14341 gccgtgacgt ggcaggcgaa tcacgcgtat gtcgtgagcg aagtatttac caccaaactg
    14401 cgcacccaga ccgagatttt gcgcttcgat cagcaattct ttttccagtt ccacatcgcg
    14461 gaatgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt tagcggaagc
    14521 cagtttcact gttttaaggt tagtttctgc agaagttcca ccaattacga acgcaatatg
    14581 atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt aatttttcag
    14641 tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt ttgccgaacc
    14701 accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat aaagatcgat
    14761 ctgcgctggc agattggtgc cggtattcac ctctttatac atatccagcg gcgcgttttg
    14821 cgagtagcgc aaattatctt cgatataagt gttatagaca ccgcgcgcca gcgccgcttc
    14881 atcacagcca ccggtccata cacgctgccc ttttttacca acaataatcg cagtgccggt
    14941 atcctgacag gttggcagaa cacctttcgc cgcgatgtcg gagttgcgca ggaattgcag
    15001 cgccacatat ttatcatttt cgctggcttc cgggtcacgc agaatgtcgg ccacctgttg
    15061 ttggtgagcc ggacgcagca taaatgacgc atcatgaaat gcctgacgcg ccaacagagt
    15121 taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata cgctaacgtg
    15181 ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag gagcctgata
    15241 atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt atgctctggg
    15301 cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt ttttaacaaa
    15361 aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat tcttgcttta
    15421 atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact cattaactca
    15481 gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg tatggaaaat
    15541 ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag cagttcacgg
    15601 gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag taataaatcg
    15661 actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct gttcaaagta
    15721 ttatgcgcag cacagccgct ctccattcag gttcatccaa acaaacgcaa ttctgaaatc
    15781 ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg taactataaa
    15841 gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc gatgaacgcg
    15901 tttcgtgagt tttccgagat tgtctcccta ctccagccag tcgcaggtgc acatccggca
    15961 attgctcact ttttacaaca gcctaatgcc gaacgtttaa gcgaactgtt cgccagcctg
    16021 ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc ggcgctagac
    16081 agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta cccggaagac
    16141 agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg cgaagcgatg
    16201 ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga agtgatggcg
    16261 aactccgata acgtgctgcg tgcgggtctg acgcctaagt acattgatat tccggaactg
    16321 gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca gccggtgaaa
    16381 caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc gctgcatgac
    16441 cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt ctgcgtcgaa
    16501 ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg tgaatcagcg
    16561 tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt agcgcgtgtt
    16621 tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc ttattaaagg
    16681 cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa tcattatgaa
    16741 taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga caggcggcgc
    16801 atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc aggcgaacgc
    16861 gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa actatcatcg
    16921 cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga aagaaaatcc
    16981 gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg gtcccttccc
    17041 gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc aaaccacgct
    17101 ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa cgccttttga
    17161 gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc tcaagccact
    17221 gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc agttaaatgc
    17281 tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc ggatagacgc
    17341 agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa ccgacggttc
    17401 cagcacgctg gcaagttttg gtgagcgcgt aggaaaccaa aaactgtcac tggaaaaaat
    17461 gaccatttca gtggaaggca aagaactggc actgctggaa agcatggaga tcaacggtaa
    17521 atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact cgctaaacag
    17581 cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag tcggccagat
    17641 tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc aggcgctgct
    17701 ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga cggaagcctt
    17761 ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg cgccgctaag
    17821 ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga aagatccggc
    17881 aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg ttaaatctct
    17941 ggatgcgaaa ctgaccattc cggtggatat ggcaactgag tttatgactc aggtagcgaa
    18001 gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag ttgaaggtgc
    18061 atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca tcaccaccag
    18121 cctgcaatat actaacggtc agataacgtt aaacgggcag aaaatgccac tggaagattt
    18181 cgttggtatg tttgcaatgc tggcattaaa tgttccggtc gtacccgcta ttccgcagca
    18241 gtaattcaca gccaaaacgc cggagatttt tctctccggc gttatttttt acttcagcat
    18301 aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc ccagcgtact
    18361 gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc cttgtaacgg
    18421 cccttcgctg acccgatagt tcataccacc taagataccc gcgccttcaa tccgcacatc
    18481 tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatatcagtg taaattgcgg
    18541 cgttaaacga tagttgacgc ccaattgcca ggactgcatt ccttcacggt tacgatcaag
    18601 acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca ttgcatggcg
    18661 caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca attgtccact
    18721 aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga gctgcgcgta
    18781 aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc ttttctcgag
    18841 aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag aatagcgact
    18901 ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat cgtattttcc
    18961 cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag tccataaaaa
    19021 tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc aataacctaa
    19081 tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta acggaatacg
    19141 ataaggtaag ctaccataac tataatccgt cccgaagcga cctaatttaa gcaggctatt
    19201 ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat caccatcaag
    19261 ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat acgccccgcc
    19321 ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc ctccctgtac
    19381 ccatgcgtaa atatctcttc ccgcgccagc actgggctta tccgccctgc gcaattcgtt
    19441 tttcagtctg atgcgtagtc ccgcttcatc gtcagccagt tgcgccgcat aagcagaggt
    19501 aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc tcatagttgc
    19561 acctttgatg ttctgattat ttttattgaa tattaattag tgatatcgct gattaattgc
    19621 tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt tttatccgtg
    19681 agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg tactaaggca
    19741 attgatgtgc ggatgcccat aataacttcc ggcgtttgca cctgattggc gatatatccg
    19801 cttaatccca aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta
    19861 aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat
    19921 tctacggtat cagcttccag tgcccacatc acagtcatag taacgccctg accaattgaa
    19981 gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca gaagaatagc
    20041 agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc
    20101 gcgaccatcc ccggcaccag cggtgccgat gccacagtac caaccaggtt ttgcaccagt
    20161 accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag
    20221 gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc
    20281 gggcgattcc gtttcagggt ttgcagactg atcttcaatg acggctgcgc cacgatacgt
    20341 accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc
    20401 agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga gttcttaatg
    20461 ctcggtccta tcagaaatgc caggcagaca aaggttaatg aagcggcaat cccgcgagcc
    20521 gcgcccagac gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag tgaaccataa
    20581 ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata tgcatacact
    20641 actttgctgc tatggctcca gtcggtcggt acccagaata ccagcacgct gaagatcatt
    20701 aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt attcacactg
    20761 tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac cagtaacagc
    20821 atggtgcccg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact caacaggaag
    20881 agcgccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta gccgacgatg
    20941 gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt tcaccgaagt
    21001 tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg cggtcgcgag
    21061 tgaagatccc tttcttgtta cctccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg
    21121 cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg cggtgataca
    21181 tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt gagtgcagcc
    21241 cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt ttctcctgcc
    21301 aggccagaag ttctttttcc agtaccttct ctgccgtttc caaatcgccg ctttggacat
    21361 accatccgta ataacggttc aggcacagca catcaaagag atcgctgatg gtatcggtgt
    21421 gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg agtttacgcg
    21481 ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta tccggttcgt
    21541 tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc agctctttaa
    21601 tcgcctgtaa gtgcgcttgc tgagtttccc cgttgactgc ctcttcgctg tacagttctt
    21661 tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg acagcagcag
    21721 tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct tcagcgtaag
    21781 ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg tggtcgtgca
    21841 ccatcagcac gttatcgaat cctttgccac gcaagtccgc atcttcatga cgaccaaagc
    21901 cagtaaagta gaacggtttg tggttaatca ggaactgttc gcccttcact gccactgacc
    21961 ggatgccgac gcgaagcggg tagatatcac actctgtctg gcttttggct gtgacgcaca
    22021 gttcatagag ataaccttca cccggttgcc agaggtgggg attcaccact tgcaaagtcc
    22081 cgctggtacc ttgtccagtt gcaaccacct gctgatccgc atcacgcagt tcaacgctga
    22141 catcaccatt tgctaccacc tgccagtcaa cagacgcgtg gttacagtct tgcgcaacat
    22201 gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagacc attacgctgc
    22261 gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
    22321 catcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa
    22381 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg
    22441 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag
    22501 tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt
    22561 cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg aactgatcgt
    22621 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct
    22681 gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga
    22741 tttcacgggt tggggtttct acaggacgta acataaggga ctcctcattc aaataaaaat
    22801 actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga
    22861 gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa aaaaccaaaa
    22921 tctgcaatac ccacagcgtg atattgatcg cattaatctt taaaacagcg ttgacagaag
    22981 ccataaaata agcaatcgaa cgttcgttcg agcatcttga gaacgataaa ctctcatgac
    23041 atatggctac ggtgaatatt tttgggggga attctccgat cccggctaga ttgtattttg
    23101 tacagggaaa ttaaagtatg aaacgcgggc acagttatca ccagcccgcg aaatgagagg
    23161 atcaggatgc ggttaagata ccgccaatca tcgcgcgtaa tccctgagca aacgacgctt
    23221 cacgcgccag gtcttccgct tcgatatcgg ccagtacacc gtaggtcaga tccagcagta
    23281 aacgtgaagt cattgacgtg ttgatgtctg ggcttatctc gcctttttgc tgggcatcac
    23341 gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt atttttaagc atggcggcga
    23401 cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt tgcccaaagg
    23461 cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga atcccttcaa
    23521 tcggttcccg gaaacgggcc agcgccctct cctggtcctg taagataatc gcctgaatca
    23581 aggcttcttt ggagatgaaa tggtgataga gcgtcccggg actaatggcg caggatttac
    23641 agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct ctggcagcat
    23701 tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc attaaaccat
    23761 tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat cattgctgtt
    23821 gaatagtgcg aaggcacact ctattcgtat gcttttcatc cagctattgg ttaaccaata
    23881 gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca cgctatcccg
    23941 gtaaacaaca catacggctg tttaccggtt cgttagtgta ttaattgagc tcctgtaccc
    24001 caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc gagcaaagga
    24061 acagcgccgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta
    24121 acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt aatattgccc
    24181 ccggcgcaat gccattcacc cgaatatttt tttcacccag gtcaaatgcc atatttctga
    24241 ccagatgact ggccgcagct ttagatgatg cataggaagt catgtttata tttttatttt
    24301 ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc atttctggcg
    24361 caacaagttg tgacagatgg aaaaaagaca acacattcag ttcataagca cggcgaaaat
    24421 ctgccattgg catatcaaac ggtttaggac caccgccacc ggcgttgtta accaggatat
    24481 caactttacc cagcttactg acggcaaagt ctgccagtgc agagagttcc tgttcggaag
    24541 taatatcaca acggcaggca aatgcctgac cacccagttg ttgaatttcg tctacaacat
    24601 ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct gtcgcgaatg
    24661 taatggcgat ttctttacca atacctgcac ccgcacctgt gatgatggcg cattttccgt
    24721 cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag taaaaataaa
    24781 aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggac actaccaggt
    24841 atagcagata aagagaggat tatttcgccg caatgagccg ggcgggaata ataagattgc
    24901 gtgaatgcat ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt gtggtaccaa
    24961 gttcccgcgc aggcgtgctg gcccaggtaa cgggtatatc atcaagtgtg gttggtgtcg
    25021 catcggtaaa tgccgccagc gaaacttgtt gctcaaagta acgatcgact ccgctttccc
    25081 cgctttgccg ccctgctttg agcaaaccaa accatgcccc catcgcaata gtttcgttat
    25141 agcaaaccac agcactgatg gtcgggttat gacgtaaaag cgccgtgata gcttccgcgg
    25201 cttgcttctg gctggatgtg cactccagca cccaatcgct gtgaaacggc aggccaaatt
    25261 ttagtagagt tgcacaatag ccccccaccc gttctgcacg ggttaatgag gaactttgcc
    25321 ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag atgctccgtc aacaactgtg
    25381 cagcctgcat gttgtccggg cgaaccgtat caacatcatc aagataactg gcacgggaag
    25441 cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg ctgcttcctg
    25501 cagccccggc aattaccaca ccatccacac cctgattcag taacagtgaa aaccgctgtg
    25561 ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc tgcgcttcca
    25621 gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg cgcagataaa tcacggacga
    25681 tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctggcga ttgcgcacaa
    25741 atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag attcgccctt
    25801 tgccactaag caccagcgaa acggtactta ccgacacgcc cgcagccagc gcaacatcat
    25861 gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt atgtgacaga
    25921 taaaacgttt taccttttat ttatcttata cccgctatta tcgttgcgta atgtgattta
    25981 tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg aatccagatt
    26041 gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca cgctgtggga
    26101 gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt cgttctgcgg
    26161 cattatgctc ggcattggta gttctcttag cagccatgat gtcataaccc tgatcccggt
    26221 cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg gctcgtttgc
    26281 ttttagtttc ctgcctgtca tgttctgtat cgccatcccg ctgggcctgg cacgcgaaaa
    26341 taaaggcgta gcggcattcg ctggcttcgt cggttatgcg gtaatgaacc tcgcggtaaa
    26401 cttctggttg accaataaag gcattctgcc aaccacggat gccgcggttc tgaaagccaa
    26461 taacatccag agcattcttg ggatccagtc gatcgatacc gggatcctcg gtgcggtgat
    26521 cgccggtatt atcgtctgga tgctgcatga gcgtttccat aatatccgcc tgccggatgc
    26581 gctggcattc ttcggcggta cgcgcttcgt accaattatc tcctcgctgg tgatgggcct
    26641 tgtcggcctg gtgattccat tagtctggcc gattttcgcc atgggtatta gcggcttggg
    26701 ccatatgata aacagcgcgg gtgatttcgg accgatgctg tttggtaccg gtgaacgtct
    26761 gctgttgccg tttggtctgc atcacattct ggtagcatta attcgcttta ccgacgcagg
    26821 cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct tccaggcgca
    26881 attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc tttcgcaagg
    26941 taaaatgcct gcgtttctcg gcggtctgcc gggtgcagcg ttagctatgt atcactgcgc
    27001 gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga tcgcctgcgt
    27061 cgttggcggc actaccgaac cgctggaatt cctgttcctg ttcgtagcgc cagttctgta
    27121 tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc tcggcgtcac
    27181 catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt tgcatggtct
    27241 gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg tttactacgt
    27301 catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg atagcgaagt
    27361 tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt acaacgttcc
    27421 tgcaatcctc gaagcattag gcggtgccga caatattgtc agcctcgata actgcattac
    27481 ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac tgaaggacaa
    27541 tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta tcgggccaca
    27601 agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg cataaggata
    27661 agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta cacagtggga
    27721 ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt cagacatgga
    27781 ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc acggcgtatt
    27841 tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact ggttttccac
    27901 ccagcattac accgcaattg atacgcagac ggtggtgtat ggcccttctg tcatctatat
    27961 ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga tccacacgcc
    28021 cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa tgcccgttgc
    28081 tttagagaag caggctgacg actggttttg cgatatgggc aagctggaag ccgtgttggc
    28141 gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg ggaaagtgtg
    28201 gacgtgcgat gagctggaga tcatggctga cctgtgcgag cgtcatggtg tgcgggttat
    28261 ttccgatgaa atccatatgg atatggtctg gggcgagcag ccgcatattc cctggagtaa
    28321 tgtggcgcgg ggagactggg cgttgctgac gtcgggctcg aaaagtttca atattcccgc
    28381 gctgaccggt gcttacggga ttatagaaaa tagcagcagc cgtgatgcct atttatcggc
    28441 actgaaagga cgtgatgggc tttcttcccc ttcggtactg gcgttaactg cccatatcgc
    28501 cgcctatcag caaggtgcac cgtggctgga tgccttacgc gtctatctga aagataacct
    28561 gacgtatatc tcagataaaa tgaacgccgc gtttcctgaa ctcaactggc agatcccgca
    28621 atccacttat ctggcctggc ttgatctacg tccgttgaat attgacgaca acgcgttgca
    28681 aaaagcgctt atcgagcaag aaaaagtcgc gatcatgccg gggtatacct atggtgaaga
    28741 aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt tcgaaactgg aaaaaggtgt
    28801 ggctggatta ataaacgcca tccgcgctgt tcgctaaccc caattgcgca acgtaaaaaa
    28861 tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg cacttttata
    28921 ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat atccatcgcc
    28981 accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag tataatatct
    29041 cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt gccaacgaac
    29101 ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc gcctctcttg
    29161 atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac ggcctgcact
    29221 atgtcgagct gcgtttttca ccaggctaca tggcaatggc acatcagctg cctgtagcgg
    29281 gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg
    29341 cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag caagagctgg
    29401 aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt gatgaacttg
    29461 gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata
    29521 ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg attcgtgaac
    29581 tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggcg ctgatggatt
    29641 ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt cagaccagca
    29701 ccgtagcaga gctggctgca catccgctga aaacgttcct tgagcatggc attcgtgcca
    29761 gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa tataccgttg
    29821 ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca ggcacagatt aatggtctgg
    29881 aaatggcttt cctcagcgcg gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt
    29941 aacaaaatgg atggtgcaaa ttgcaccatc catttttcat gcaaggcaca aagtcgcgcg
    30001 atgtttggcg gattcgatgc ccagctcaat caactccatt acctggattg cctggcttgc
    30061 cggaaccgga ttttcaccat cgccatttaa cgcatcacga atagccgcat agtaagccgg
    30121 ataattccca ggcaccgtca acagcgtttc ttcgacacgt tcctcacctt ccacgcgggt
    30181 aagtacgcca tcacgcatat cgtagcccca gtcttcctgc ggtagacgct cgccattttt
    30241 cagacgttct tcctgtggat cgaggccata tttcacataa ctgcctcggg atccatgcac
    30301 gatataccgt gctgactcag cggctgccag catggtaccg tgtaaaatga ctcgccgctg
    30361 tggataggac aagatggcgt ggaaataatc ggtcgactgc gctccgggcc gtaactgtgc
    30421 caaatctacc gtcatgctga ccggtaaacc aaaaagcgta atggcctgat caagaagatg
    30481 tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc tgttcacgcc aacgatcgcg
    30541 cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa gcaacttcac ccagcacgcc
    30601 ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc cagcgacggt tatggaatac
    30661 agacagcaca cgccccaggc tttttgccag cgcatccagc tctcgcgctt gtgacagtgt
    30721 cacggtaaag ggtttatcaa cgaccacatg tttacccgcc tcaagcgccg ctttggctaa
    30781 cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg tctatgttgg gatcgttaaa
    30841 cagatgcttc ggctcagaga caaccgtaac cgttggccag tcggctttta cttttgtttc
    30901 atcactgctg gagattaccg ccagttccag cccgggcgtg cccgcaatca ggggcgcatg
    30961 gaaggttttg ctcgcataac catacccaat caacccaaca cggatgttgt cgctcatgtc
    31021 attgcctctc attgaagtat gatggctatt tgacactatc ctttacccac gctcaacagt
    31081 ttaataacct gccagcaata agggatgttg tttaacttaa gtcaaaaaaa tagcgaattt
    31141 tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa aatcttgctg gagctattat
    31201 tgctaagtaa catttacccc ctgaagttaa tggatcaatc aagagagatg tgggctgtaa
    31261 tgaatcgtct tattgaatta acaggttgga tcgttcttgt cgtttcagtc attcttcttg
    31321 gcgtggcgag tcacattgac aactatcagc cacctgaaca gagtgcttcg gtacaacaca
    31381 agtaagctct gcacttgtgg agcgacatgc tgcccgtccg ggtgcatgtt ttcacttgtc
    31441 ggatattaaa ccaggaattt attatcttgt tcgatgttgt tggtgattgt cagggatagt
    31501 aaagcgcgtt actcttttca gagcagtttt tcatccctga atatttgcca tcagcgcatt
    31561 cttattattg taataacaca agcgttcact tttggtgaaa ttttatcatc actaataata
    31621 cctacccctt tggtattaat tattttatgg accttttatg actgttcagg actacttatt
    31681 aacatttcgc aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta
    31741 caccctgacg gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc
    31801 agagctggtt tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca
    31861 ctatgtccaa taaagaaagt agcgatcggt tataaaagcc ttataataac gcccctaaaa
    31921 ttagatttgc ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac
    31981 gtattggagg ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga
    32041 cgctggcgtt gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg
    32101 gcggacaagc actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca
    32161 tgtggtatta cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta
    32221 aacactatat tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt
    32281 cacccgtgga agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat
    32341 tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat
    32401 aaccttacag ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt
    32461 atttcaggcc gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat
    32521 aactttgtac tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg
    32581 gaaaccgcga tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc
    32641 gcctggctta tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg
    32701 gcatttattc tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc
    32761 agcccggtgc tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca
    32821 gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg
    32881 tacggttttt ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc
    32941 gaacgccttg ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt
    33001 accgcaggtc ttatgtctct ggcctttatg ggctttagtg gtttggtgaa gttgtaatga
    33061 atgctatctg gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc
    33121 tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg
    33181 aaatcttacc gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg
    33241 aagccatcag ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga
    33301 tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag
    33361 agctaacgcc tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta
    33421 aatgtattca ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg
    33481 taatgagtga tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca
    33541 tctcgttgca accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc
    33601 ccgtgcgtat cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat
    33661 aaaatctggg atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt
    33721 acacccctgc gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt
    33781 ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt
    33841 acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct
    33901 attgcgcccc actctacggc tcatccttca gctttagctg aattaagcgt gattattgat
    33961 gccgatggtg aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt
    34021 cgcgaagagt taatcgagcg catacatcag tttggtgtag ccgggctggg cggtgcagga
    34081 ttcccgacag gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac
    34141 gcggctgagt gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct
    34201 caggtcgtag agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc
    34261 ggcattgaag ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct
    34321 aacgatattt ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta
    34381 acctacattc tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta
    34441 ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg
    34501 attaccgagc gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg
    34561 gcacggctgg ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc
    34621 gatcaaatgg tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc
    34681 ccggtcgtaa agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca
    34741 caggaagaac aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt
    34801 ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat
    34861 aacattgctg attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc
    34921 ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag
    34981 cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa
    35041 gcggctcgcc ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat
    35101 gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt
    35161 gtgattaaag cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt
    35221 aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt
    35281 gctgatccac gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAHBHC010000053        34899 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHBHC010000053 DAHBHC010000000
VERSION     DAHBHC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.053"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(61..1683)
                     /locus_tag="M2A35_003017"
     CDS             complement(61..1683)
                     /locus_tag="M2A35_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCB7199285.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPVLKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            complement(1686..2258)
                     /locus_tag="M2A35_003018"
     CDS             complement(1686..2258)
                     /locus_tag="M2A35_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCB7199286.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(2320..2844)
                     /locus_tag="M2A35_003019"
     CDS             complement(2320..2844)
                     /locus_tag="M2A35_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199287.1"
                     /translation="MKLTYKIVIAAFFFSVFGALVWSANHYHSKYQAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            complement(2828..3304)
                     /locus_tag="M2A35_003020"
     CDS             complement(2828..3304)
                     /locus_tag="M2A35_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCB7199288.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            complement(3308..3649)
                     /locus_tag="M2A35_003021"
     CDS             complement(3308..3649)
                     /locus_tag="M2A35_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCB7199289.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAICGAAMR
                     VIGNKFGGSKE"
     gene            complement(4095..4436)
                     /locus_tag="M2A35_003022"
     CDS             complement(4095..4436)
                     /locus_tag="M2A35_003022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199290.1"
                     /translation="MALISVRNRFESFMEQRYPDLSLQVKGNIGASMKAQLGIRIERE
                     LYSEDVTYCDSAVQLMWTLFQAGVLAERRATSVTLPALKAKPDSFYDAGYNEGIQDCR
                     KHLTASGIKVR"
     gene            complement(4468..4890)
                     /locus_tag="M2A35_003023"
     CDS             complement(4468..4890)
                     /locus_tag="M2A35_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7199291.1"
                     /translation="MRNIQQVLERWGGWAASEGGSVYFPPVAAGFKNLLPATQSGRLK
                     CSDNDGLIINSAMSCLKKKDPYLCTLLEWHYVQAMPVRAMGEKLGVSHTHVLKRLQAA
                     EGFIDGCLAMLDVVLEMDQSVQSKPQAIRTLRRSCSAA"
     gene            complement(5179..7365)
                     /locus_tag="M2A35_003024"
     CDS             complement(5179..7365)
                     /locus_tag="M2A35_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7199292.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTAHLLPVVCNPSATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGELPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTAYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSTEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRPDFVDIDIRFDQFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAITWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKKCKVWEPLEPRGTT"
     gene            complement(7358..7540)
                     /locus_tag="M2A35_003025"
     CDS             complement(7358..7540)
                     /locus_tag="M2A35_003025"
                     /inference="COORDINATES: protein motif:HMM:NF025900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7199293.1"
                     /translation="MKKAEAIELAGSKAKLARLLKVSKGAVSQWGEEIPELRSLQLEK
                     ILEQKNVVKQKGLTHV"
     gene            7654..8346
                     /locus_tag="M2A35_003026"
     CDS             7654..8346
                     /locus_tag="M2A35_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7199294.1"
                     /translation="MINDRIREARRNVHLTQDALAKRIGLTKATISQWESGNTTPNGK
                     NLINLAEALNVSPEWLLSGKTSDTKEIHSNAQVEGGFSVWDSSTPLEDDEVEIPFYQE
                     IELSAGNGTYVDIDRMGCKLRFARSTLRKAGVDVNCAACVSIHGNSMEPVLPDGAVVG
                     IDTSKTGVKDGQMYAIDQDGLLRVKLLYRLPSGLRIRSFNRDEYADEEYFNQEANKIR
                     IIGQVFWYSVLL"
     gene            8994..9143
                     /locus_tag="M2A35_003027"
     CDS             8994..9143
                     /locus_tag="M2A35_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199295.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            9140..10042
                     /locus_tag="M2A35_003028"
     CDS             9140..10042
                     /locus_tag="M2A35_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199296.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATETENMSAAMTLDNLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRAKALHKSALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            10045..11346
                     /locus_tag="M2A35_003029"
     CDS             10045..11346
                     /locus_tag="M2A35_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCB7199297.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGSELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWALTVEELQAFGERAQEAATS
                     AILVLNIAECEGIETLPLDNFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTIVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            11362..11910
                     /locus_tag="M2A35_003030"
     CDS             11362..11910
                     /locus_tag="M2A35_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCB7199298.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKKVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(11937..12536)
                     /locus_tag="M2A35_003031"
     CDS             complement(11937..12536)
                     /locus_tag="M2A35_003031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199299.1"
                     /translation="MEIKDVIALVGLIIPGITAAVGVYKLLNDVSWFLSKTTKFSHIL
                     ENYREYIDPSELEFMKAEIKREIKKSILGMVNLKLRPLILYIRTYSELDMPSWRWWFL
                     APHIQCKYDRFFIRYKGKYKRYRLHSKIAAIFYLGYGLIFLCLLLNKGTVYIVLGVVV
                     MLFCLFTFFMFWTLFPGREVINKYNLQLLKVDASKYQVK"
     gene            12563..14626
                     /locus_tag="M2A35_003032"
     CDS             12563..14626
                     /locus_tag="M2A35_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCB7199300.1"
                     /translation="MSNILWGDLETYCEIPITNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDVELARAAIDAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEKWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITESPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            14632..14847
                     /locus_tag="M2A35_003033"
     CDS             14632..14847
                     /locus_tag="M2A35_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCB7199301.1"
                     /translation="MPSKYRDNPLYYRAAREALRLEQFGEYDRAAKAWAKANRESHHE
                     LNQEWSERRSDFCLMQNMREKRKAVEE"
     gene            14844..15143
                     /locus_tag="M2A35_003034"
     CDS             14844..15143
                     /locus_tag="M2A35_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCB7199302.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPNGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
     gene            15133..15402
                     /locus_tag="M2A35_003035"
     CDS             15133..15402
                     /locus_tag="M2A35_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCB7199303.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGHRSVPDRIVLLPG
                     SRIVFVECKSPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            15399..15575
                     /locus_tag="M2A35_003036"
     CDS             15399..15575
                     /locus_tag="M2A35_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCB7199304.1"
                     /translation="MSKRDDPQLRVRIPQDLKDALEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(15544..15873)
                     /locus_tag="M2A35_003037"
     CDS             complement(15544..15873)
                     /locus_tag="M2A35_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCB7199305.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQAEWVYENKYELIEAMEKLKK
                     LLNPENE"
     gene            15990..16166
                     /locus_tag="M2A35_003038"
     CDS             15990..16166
                     /locus_tag="M2A35_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199306.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            16226..17032
                     /locus_tag="M2A35_003039"
     CDS             16226..17032
                     /locus_tag="M2A35_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7199307.1"
                     /translation="MNTLTLINDTKQPMMGSRKIAEVTGKQHKHVVRDILAMLEQLQI
                     YSPDLDDDDFKGFIIKRKEYKGRDVIDEIWLDENLSMTLVTGYDAKRRLALIEQWQAM
                     KIELEQPRPASLPVPAPAIQVNDNIVQFARVVAEATASATMKAIVEVMAPRQVQNVTL
                     STPGHETNWVEDLKQQVRESQPHSNGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRS
                     DTGVRGLLVKLPAIEQAAQKLLNESTPPKGNRKRWRHPQFGNFTYYTDLI"
     gene            17076..18476
                     /locus_tag="M2A35_003040"
     CDS             17076..18476
                     /locus_tag="M2A35_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCB7199308.1"
                     /translation="MGQIFTPRPYQDLIINHQIETLRGNIWAGMGMGMGKTVATLTTL
                     EDLFMAGAETRPALVLAPLRVAANTWPDEAMKWGHLRNIEVQPIVGNAKARSAALANS
                     NASVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKY
                     VRRWINLTGTPAPNGLVDLWGQSWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLE
                     PFAHSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLE
                     LNGEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVL
                     VAYHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGN
                     ILVFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKR
                     TVQDILLDAMKKRGIA"
     gene            18473..18673
                     /locus_tag="M2A35_003041"
     CDS             18473..18673
                     /locus_tag="M2A35_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7199309.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(18670..20067)
                     /locus_tag="M2A35_003042"
     CDS             complement(18670..20067)
                     /locus_tag="M2A35_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7199310.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASGKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            20282..20542
                     /gene="yfhL"
                     /locus_tag="M2A35_003043"
     CDS             20282..20542
                     /gene="yfhL"
                     /locus_tag="M2A35_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCB7199311.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20736..20816)
                     /gene="shoB"
                     /locus_tag="M2A35_003044"
     CDS             complement(20736..20816)
                     /gene="shoB"
                     /locus_tag="M2A35_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCB7199312.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21236..21616)
                     /locus_tag="M2A35_003045"
     CDS             complement(21236..21616)
                     /locus_tag="M2A35_003045"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCB7199313.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21616..22347)
                     /gene="pdxJ"
                     /locus_tag="M2A35_003046"
     CDS             complement(21616..22347)
                     /gene="pdxJ"
                     /locus_tag="M2A35_003046"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCB7199314.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22359..23087)
                     /gene="recO"
                     /locus_tag="M2A35_003047"
     CDS             complement(22359..23087)
                     /gene="recO"
                     /locus_tag="M2A35_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCB7199315.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(23099..24004)
                     /gene="era"
                     /locus_tag="M2A35_003048"
     CDS             complement(23099..24004)
                     /gene="era"
                     /locus_tag="M2A35_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCB7199316.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(24001..24681)
                     /gene="rnc"
                     /locus_tag="M2A35_003049"
     CDS             complement(24001..24681)
                     /gene="rnc"
                     /locus_tag="M2A35_003049"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCB7199317.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24954..25928)
                     /gene="lepB"
                     /locus_tag="M2A35_003050"
     CDS             complement(24954..25928)
                     /gene="lepB"
                     /locus_tag="M2A35_003050"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCB7199318.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25944..27743)
                     /gene="lepA"
                     /locus_tag="M2A35_003051"
     CDS             complement(25944..27743)
                     /gene="lepA"
                     /locus_tag="M2A35_003051"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCB7199319.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27941..28420)
                     /gene="rseC"
                     /locus_tag="M2A35_003052"
     CDS             complement(27941..28420)
                     /gene="rseC"
                     /locus_tag="M2A35_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCB7199320.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28417..29373)
                     /gene="rseB"
                     /locus_tag="M2A35_003053"
     CDS             complement(28417..29373)
                     /gene="rseB"
                     /locus_tag="M2A35_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCB7199321.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29373..30023)
                     /gene="rseA"
                     /locus_tag="M2A35_003054"
     CDS             complement(29373..30023)
                     /gene="rseA"
                     /locus_tag="M2A35_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCB7199322.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(30056..30631)
                     /gene="rpoE"
                     /locus_tag="M2A35_003055"
     CDS             complement(30056..30631)
                     /gene="rpoE"
                     /locus_tag="M2A35_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCB7199323.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30628..30783)
                     /gene="rseD"
                     /locus_tag="M2A35_003056"
     CDS             complement(30628..30783)
                     /gene="rseD"
                     /locus_tag="M2A35_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCB7199324.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            31039..32661
                     /gene="nadB"
                     /locus_tag="M2A35_003057"
     CDS             31039..32661
                     /gene="nadB"
                     /locus_tag="M2A35_003057"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCB7199325.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGLDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(32646..33383)
                     /gene="trmN"
                     /locus_tag="M2A35_003058"
     CDS             complement(32646..33383)
                     /gene="trmN"
                     /locus_tag="M2A35_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCB7199326.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            33515..34849
                     /gene="srmB"
                     /locus_tag="M2A35_003059"
     CDS             33515..34849
                     /gene="srmB"
                     /locus_tag="M2A35_003059"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCB7199327.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         8935 a         9113 c         8423 g         8428 t
ORIGIN      
        1 tctcataacc atcagctgtg gcctgcgcag ttcgccgcag ggcttgtttg tttcgtgcca
       61 tcaggcctgt ctccctagca gctcccagat gttcagggct gaattcattg gggcatagtt
      121 gatcatcacc gagtcggcaa ggtttggcga tcgggttcca tcaggctgtt tatcaataac
      181 tatttttccc acaccattaa tggaataggt cggctgcgaa agctcgatga tgagtttatc
      241 tttgagtgcc atgctactgc tgattgagat gatttcgtcc gggttgtaag ccataccttc
      301 aaccacggcg cgccaggtat tctgaaaaag tttacgtaac cgccaccagc tctgggcttt
      361 ggcgttagcg aagaagtcct tgttcagacg cgcggcttgc ccgttgtccc cgcgaacagc
      421 ttcatcatcc ggatcaaata ccgcgccact acctcgaaac ggtgtggcaa gtattgacgg
      481 tcgacgcgca gcgttacgca gttcgttgat agcgcgtgca tcgccgcgaa cgccagcgcc
      541 cagcccgtcc tcgtcaaagc gaaactcttc gaggttgtcc tgttcgcaaa agccgaaaac
      601 cttctcgacg gactgataaa tgtcgctgcc cacaccggac cattcccgca cattttccag
      661 gaggaagcca tgacgggtgg aaaaggcatt tttgtccctg ccttcgtcgg cgacatccat
      721 cgcgccaagt cgtttgcctg ttggctggat acccagtttg atatgcgcat caacggcagc
      781 ctgtacccat tcggatggaa tcagaacgcc ttccgctgat gcgctgtagt tcagatcaag
      841 ttcctgtgcc accaccaccg gattatcgat tttctcgcat tccctgcgat accactcttc
      901 atccttgcga ggatcatccc gccagtggaa tgtgaatacc ggtatcttcc cgccatgacg
      961 cttctgagcg aacgggttag ccatgccgtt aactgaactc aggtcgatac ggcaacgcgt
     1021 cgtttgtgac aacgctgcat caatcagcag aggacgctga aggaatgcag cctcatcaac
     1081 cagataaagc gtggtacggt caccacgacc aatattatcg ccagcctcgc ctttgataac
     1141 ggcaccagtt tcaggaaact caacacgcat atatggcgcg tgcttcttct cgctccacga
     1201 accgcgaaac tctacaggta gcgtttccac gaacttgcgt gccttccaga acaatgcttt
     1261 cgggtcacca gtgctgtcga cgtattcctc tttacgggag ccgaaaccga taaccatttc
     1321 tttgttgaag agacaaagcg agcaggccag tccgatcgcg gtccaactga gccccatttc
     1381 acgggatttt tcggtaatac cattctcccg attgccccag cgttccataa tccagtggat
     1441 ccactcctcc tgcttaggga agagtaaaaa cggaatggtc accggcaggc cataatcaat
     1501 attacgcggg tccgttgtca tgccccagtc gatgatgaac tgagccggat tggttcggta
     1561 aaactgcttc aatacgggca atatttcagg attctggcga atgcgctgta ggcgttccat
     1621 ccgccattca aaaaccatct ggtaatcagg atgtttaaaa tcgaagggga atggtaacgg
     1681 catacttagc ccatcatttt tctatacgcc tctgcagcct gctccggcgt taagttggta
     1741 atttctgttc tgacgggtcc tccatcagcg ccagtcactt catttttgac gttgtcttta
     1801 aacgcctgaa cagaaacatg acgcccaagc aactcaaggt ttttaacctt atcaggccac
     1861 ttaatctttt taaggatccc gaccatttct ctgtcatctc ctcgcccctc aaacatttca
     1921 gcgaggttaa atccactcag gtaccgacgc cacgattccg gccacgcaga cagaggctta
     1981 atacttaaat cgtcctccag gatgtcagcc acatcgagcc tgtcgatctc aaccagtcgc
     2041 atcagcacat aattcgcatc aatgcccagt tgatcaatac gctcctgctt tagctcgtta
     2101 atacgggcgc gtatctcagg tttaccgtat agttcagccc ccgtaacatg tgctcgcctg
     2161 gagacgtagc ctgcgcgaat agctgcttgt gtagcattca gatcgacaag aaactcgcga
     2221 caaaacacct cgtgttttgc tttcagcttc ttagtcattt tattttccag ttatcaggtc
     2281 attatcgaag cccctcctgg aagagcttct gtaatgctat taccgggact gttctatttg
     2341 tcggacacca gccaactggt tattcgcctt ctcgatggct gccaacagtg ggttaatcca
     2401 cagaacagcc tggcaatatg tcaacgttct ggtggtagtg gcacgatcac cggctgagtc
     2461 aatgtccccg gaatcggcgt gcaatgcgct ggcgcgtaaa cggttcgcgt agttgagcaa
     2521 cccgacagca atatcaacag gaacagggaa atcacagttc ttttcacgga gcagaacctc
     2581 acggtatttg atgactgtct tctcgtgacc gatgtcgacc agagaattta atcggcttgc
     2641 gttttctgct atctggttaa aacgattgaa gtttaatgcc tgattagcta tcactttccc
     2701 ttgatattca gcttcacctt ccgctttatc agcccgcaac ttttctgcct gatacttgct
     2761 gtggtaatgg tttgcagacc agacgagcgc cccaaagaca gagaagaaaa atgccgcgat
     2821 gacaatctta taagtcagct tcatttacca ccccaccagc atctttaaac ctggaaatca
     2881 ggtcaccgat tttatgttca tactgaccgt aacctgcacc aggtaacgac gcccagatat
     2941 tgctgcaacg gtcgattgcc tgacgaatat cgccacggtc aatcatcggt aaagcgccac
     3001 gctctttaat ctgctgcagc gccacagcgt cctgactttc tggcgaaaaa tctttcaggc
     3061 caagctgctt gcggtaggca tcccaccagc gtgaaagaag ctgataccgg cctgcggctg
     3121 ttgatttaag cttcgggttt agcgtgacaa gtttgcgagg gtgatcggag taatcagtga
     3181 acaactcacc tccgacgata acgtcataac cgtgattacg tgtcggttgt cgcccgttat
     3241 ccgttccttc tgaccatgcc accatatcca ggaaagcttt acgttgggaa tttagtgtct
     3301 gcatgaatta ctccttagag ccaccaaact tgttaccgat tactcgcatt gcagccccac
     3361 aaatagcatc gacaccgatc agcccaacac caccaccaat ggcaacagaa agcgatttag
     3421 gccatccgac atactcaaga gcggatgcaa aagtcagcgt cagagcacca cagagcaaaa
     3481 tctcaagcgt ttttcgtttc cagccgccgc caccgccaaa ataggcaatg cgcaagccag
     3541 ccataacaat tgacataacc actgcgccca acggcgtatc tccacgccac caactttgta
     3601 agagttccag taagtcaggc caggaatgag gagcattgtg cattttcata cttcccacct
     3661 ccgccattac ggggtgttgt tgaaaggcgg gccctgcgta tacgcccgta ggattgggtt
     3721 atgagccgtc cttcggtggg ccctgaatac aaaaaaagtt cgccatagcg aaccttgtta
     3781 aatttgttaa aaaaggaagt tatttaacat aatgtacgtt ataggaacca cacgatcccc
     3841 gctcgcaata gatttgcgat gaaaggccta tttaatcaac ttaagtggtc cagaatgacg
     3901 aaattcgagt gaataaaagg tgcataaaaa agggcaaaaa ctgcatagcg ttttttcgca
     3961 gcgaaagccc tgttttatta acttttcccc agaatggggc aataaaaaag gcccccttgc
     4021 ggaagcctct tcgtgatgaa ctgttttcaa cagatatcag gctaatcggc tttttggaat
     4081 tccacaacta ttttttatct taccttgatg cctgatgccg tcagatgttt acgacagtcc
     4141 tgaatacctt cgttataacc cgcatcgtag aagctatccg gctttgcctt cagcgcagga
     4201 agcgtaacgc tggtagctct tcgttcggcc agaacacctg cctgaaagag tgtccacatc
     4261 aactgaactg ctgaatcaca gtaagtaaca tcttcactgt acaattctcg ttcaatcctg
     4321 atgccaagtt gagccttcat tgatgcgcca atattacctt tgacctgcaa cgacaggtct
     4381 gggtacctct gttccatgaa gctttcaaat ctgttgcgca cgctgatcag agccatctgg
     4441 ttcccttacg tgattgattg ctggatatta tgccgccgag cagctcctac gcaaagtcct
     4501 gatagcctga ggctttgact gaaccgactg atccatttcg agcaccacat ccagcatggc
     4561 taagcaaccg tcaataaatc cctctgccgc ctgaagcctc ttcagaacgt gggtgtgaga
     4621 tacgccgagc ttctcaccca tcgcccgcac gggcatggcc tggacgtaat gccactcaag
     4681 gagcgtgcac agatacggat cttttttctt caggcagctc atagcggagt tgatgataag
     4741 accgtcattg tcactgcatt tcagccttcc agactgcgtc gcgggtagca gattcttaaa
     4801 cccggctgca acaggaggaa agtagacgct accaccttca ctcgctgccc atccacccca
     4861 gcgctctaaa acctgttgaa tattacgcat agctctgacc tctacgtttt gctacgaaaa
     4921 atttttctga gaactacgtc tgtttatccg tgggtgtgga taacgcagtt ccgatccgca
     4981 tattgcatgt atcaacatgc aaaaagttgc taattcggag tggaaccacc tgcccccacc
     5041 tggaaccacc ttttcctaac ctttccctca atcgacttat atatatatat ggggtttcta
     5101 gtagaaaggc ggttccagtg gttccagtag ttccgccccg cttgccacaa ggtctgcaag
     5161 gtggaaccac cttctcttct aggtggttcc gcgtggttcc agcggttccc aaaccttgca
     5221 ttttttcccc ccaattcgcc tttgggcacg cttatacccg caattttgca aaacattact
     5281 aattcgcatt tcttcgcgtt ttccgatgcg gtcaggattt agcccaatcg catcacgcaa
     5341 aacatcactt gcgcgtaaaa attcgcaatt tcgcggaagt tcattagtca tcaggtcggg
     5401 cgtgtcgagc catttctcta ccgtttcaag ccaagcatcc ttgatggtgt actgttcatg
     5461 gacactcgct gccagttgtt cagcctcacg gaactggata ccccccagac gctgaaacac
     5521 ctcacgagcc tcagcccaaa gtaaaaggag atctcttttt atcgctttca cgtcgacttt
     5581 cgacacttcc acggggagcc agcgacggtt accagtcttg tccgcaagga attcgtcctc
     5641 attggtggta ccaacgaaca ccaggcgacg aggaaactgg gtggcgaact cccggtattt
     5701 tgggatccag ttttcgtgag tacgcgtcac aaacgctttg attgactcca gctctttggt
     5761 attaagtccg cgcagttcac caatctctgc caccagccga ccacgcattt ttcgagcgag
     5821 atcatcgtct ttttcggcaa aagagatttc agtaaaaaac gcaggatcag ggctcagtgc
     5881 ctccactccg gaagacttac cgcagccctg cggaccaacg aggatcggca ccatatccgc
     5941 tttgatgcct ggctccagta ctcgccccgc caacgcggtc cacatgtaca tagacaccgc
     6001 acgggtataa ggcgtgtcgg ctgtaccgaa gtgcgtatgg tagaaacatt cgatgcgcgg
     6061 cacgccatcc cactccagcc cgttcagcca ggtgatcgct gaatcgaatg gttgttcatc
     6121 ggctgcaagt aacaccacat cgcgaataag ttcacgacca acaggtttaa atccccgctt
     6181 ttccatcgtg atgcgcaggc gcgcataatc cgcatcggtg aatgctcgcc attgtccgga
     6241 tcctgcaggg gcaaacatga tttcgtcgcg gaactggtca aagcgaatat cgatgtccac
     6301 aaaatcaggg cgtactactg ctttggctgc gttgctgatg gttgcctcga tacgccccca
     6361 tttatcacgc tcgaaagcag gcagcggtaa aggttccgcc acttcggtgc tggtcagatc
     6421 ttcgaaatcg tcgttgcgga tcccgatggc attaaggaaa tctccgtcat cacgatgcgc
     6481 acaactggca tgcaggcatt taaaatgccc ctgctcaaag cccgcggttc ccgcaggaaa
     6541 ataagctgtg cttgttggat cgcccccgct actgtggccg tcttcaaacg gacagcggat
     6601 atatcgttca ccatttgcgc catccagcag cgtccagcca ttggcatcaa gatattcagc
     6661 tgtatcatcc gtcgcgccgg gcgtgaatgc tgatcggtcg cgcatcttcg tgttgcccgc
     6721 ttcggtggtt accgacacag ggagttgttc agccaggcgc tgccacagcg tttcaagctg
     6781 ctcaccagta atagccggag gttcatccgg caaaccaccg tcccattcaa tacgcgcgcc
     6841 gctgctgtgc gtaccacagg caacgaactg ctgcccgttc gccagcaact cgataatgcc
     6901 catatccccc gccaggcgat ggatacgctt acggaaatca ccatcaacgg ccagcagata
     6961 cagacactta ttactgtttg ctcgccagcg tcgcggcggc aactccccca gaagttgcac
     7021 aagcgttttg cgaatatctg cctgaatgtc ttcatcttcg ctatcgcagt ccagcgccag
     7081 ccagccatgg cctgtacgca cgcagatgcc ataatccggt tcattcgacc agcgggcaaa
     7141 atcatgctca gtaacaacat gcccggtcca ttgagcaata ccggtgacct ggcggtcccg
     7201 gttatagcga ctcggcgtct tacccaacgc tttcaattta ctatcagggg atatggtcgc
     7261 actggggtta catacaactg ggagaagatg agcagtccgc cccaatacca ggtcgaaatg
     7321 aaaccactca tcaggcgtag ctccccatgg tttgctatca gacatgggtt aagccttttt
     7381 gtttgactac atttttttgc tccaaaatct tttcaagttg aagagatctt agctctggga
     7441 tttcctcccc ccattgagat acggctcctt tggaaacctt caatagtctc gctagtttcg
     7501 ctttacttcc tgcaagctca attgcttctg cttttttcat aaccgttacc caggaaatat
     7561 atggttatat gaggttaaga aaactaaacc aacaagtcaa gaaagcgata ccctgattaa
     7621 gttaagctaa ctaaactata tgagggtaaa accatgatta atgatcgtat tcgcgaggct
     7681 cgtcgcaatg tccacctaac gcaggatgcg cttgctaaac gtattggtct aactaaagcc
     7741 acgatttcac aatgggaatc cggaaacacc acacctaacg ggaaaaatct tataaatctg
     7801 gccgaagcat tgaacgtttc tccagagtgg ctactttcag gcaaaacaag cgataccaaa
     7861 gaaatacatt ctaatgcaca agttgagggg gggttttctg tatgggactc atcaacgcca
     7921 ttagaggatg atgaagtgga aataccgttc tatcaggaaa ttgaactatc tgccggtaat
     7981 ggtacttatg ttgatataga tcgaatggga tgtaagttaa gattcgcccg ttcaacgttg
     8041 cgcaaagcag gtgttgacgt aaattgtgcg gcttgtgtca gcatccacgg caacagcatg
     8101 gaaccggtat taccggatgg cgctgtcgtc ggcatagata catcaaaaac aggtgtaaaa
     8161 gatggtcaaa tgtacgctat agaccaagac ggattacttc gtgtcaaact attgtaccgt
     8221 ctcccaagcg gactccggat acgctctttc aatagggatg aatacgcaga cgaagagtat
     8281 tttaatcaag aggctaataa aatcaggatt ataggccaag tattttggta ctccgttctg
     8341 ctctgagcgt aaattcactc tataccacat gcccgcttcc taagcgggct tttctttacc
     8401 catccgaaaa agttcacttt tcttaactac aacacttgac tttgtgagtt tagagatctt
     8461 aactatatgc gtgtagttaa gatatctata cacagatatt taaaccacaa gtcgaacggc
     8521 gcgactctaa accatgcgtc gggaccgtgg cgggacagga tgtcggcaat acaggtcagt
     8581 aagttccctt tggggtgcgg cgaagcactg tctaattaat acaagtcgaa gccgccgaac
     8641 caccaaagtg aactgaatga ggaatcacaa gatgcctaaa tataaactgg ttaatgacct
     8701 ggctatcggc gacaaaattg aattcagtgg aaaacggata atggagatta cccaaaaaac
     8761 tgatcgcgac cagcataacg ttagcctgac tctagttaac aaacatacag gttctgcgac
     8821 caatacttgt tttaaccgaa tggataccgt cgtccttcac ccataacaat cctctgaatc
     8881 ctccatgtaa ttgctgtgtg tagtctttgc ccgcctcaag tgacgggctt ttttatgtct
     8941 gaaagcgcac tcgcaacagc gtgctccccg atatgaaaaa aggaatacaa ccgatgaaac
     9001 ctgaacacat ccatcgactg acggggcgcg atgttctccg ttatcgccgt aaaaacttcg
     9061 atttgatgac cggtctggcc gttgccacag ctctcggtct gatcatcact ttcattctcc
     9121 ttgtagcgag gaccacagta tgagtttaga aaccagtcta gaacttaata atcaacttct
     9181 ggcacaacat aatgcgctgc ttgaacgtct tatccgcaca atggcatcag gtattgttat
     9241 gcatcctgac acgatttcac gagtgcagga atgtcgggat acagcaactg aaactgaaaa
     9301 tatgtcagcg gcaatgacac tggataatct ggagtttagc gacgttatcg cactggctgg
     9361 tttctacccg gtagccactc ctatcacaga agacatgctg caacgtgctg ttgcctaccg
     9421 tgatgctgaa ggcgataaac gagtagttca gattgatgct ctcgacagcg cattgcaggg
     9481 cgtcaaacga gccaaggcac tgcataaatc cgctcttctg gacctgtccc gtaacattct
     9541 taagttctgg gacgacctgc caaccatcgg cgagcgacgt gcttttgccg agcagctact
     9601 tgatgcacct gcagatgggc gtgatgaagt taagccgaaa aaggccagta acaaagatgg
     9661 agaacgcacg gggccgtttt acgtcaaaaa tgtatccggc acagcagcca gtgaactcca
     9721 caccttacgc aagttgaacg agatgcttaa aaaaggccat atcgagatca accgtgttga
     9781 gtaccttcag ctgcaggaag aatttgcacg cagagacgca gcaaattcca gccagaataa
     9841 tgacgccaaa gatgaccata caattgattt cgcggcacta cgcaaacaag ctgaagggtt
     9901 gatcctccag ttagcaaaag ggggttaccg ggcagaagct attgcaattc tggaaaaaca
     9961 gggagccagg aaacttggtg aagtaacgga tgaaaatctc gctgaagtaa tcaccctggc
    10021 tgaaaaagca ctggagggtt aatcatgcca gacgttcatg cacaactttc tccatcatca
    10081 gcgcatcgat ggatgcgctg cccaggaagt ctggcgctgg aggccacaca accggataaa
    10141 gaaacaactt ttgcaatcga gggcactgca gcgcacgcgc ttgctgaaaa agttctacga
    10201 aacaggcaaa gccacccgga acactacgcc ggatgcaatg tttctatgtt tctcggctca
    10261 tacccccttc gcgaaaatcc tgatgataca tccggcccac aggtggatga tgaaatggtc
    10321 gaagccgttg gtcggtatgt tgatacggtc tggactcttg cacagaataa tgaactattg
    10381 gttgaacaac gtgttgattt ctcacatata acgggtgtgg aagaatcttt cggaactgcc
    10441 gacggcataa tcattgctgg tagcgaatta caaatccacg acctgaaata tggcaaaggc
    10501 gtccgcattg atgcagaaca aaatgagcaa ctacaactgt atgctctggg tgcgctcgaa
    10561 caattcagca tgctgtacga cttcgagacg gtgcgcctgt tcatccacca gccgcggctt
    10621 aaccacgttt cagagtgggc tctgacggtg gaagagctcc aggcgttcgg tgaacgggca
    10681 caggaggccg caaccagtgc gatccttgtt ctcaatattg ctgaatgcga aggcattgag
    10741 acactaccgc tggacaactt catacctgga gaaaaacagt gccgcttctg taaagcaaaa
    10801 gctatttgca ctgcccagaa aatgcagcat ttacaaacag cggccagcga ttttgaagat
    10861 ctgacaaagc ctgtcagcga aataatcacc aatgccagcg cacgtgtacc tctgttaacc
    10921 attgagcagc ttgcggagat ctatagccag gccgacttta ttgaatcctg gctaaaggca
    10981 gtacgagacc gggttcacaa tgaactcaat gccggacatc cggtaccggg gtttaaactg
    11041 gtaacaggaa aacaaggtaa ccgggcctgg agtgatgaag aggcagctcg cgcacttctg
    11101 aaggaccagt tcaggtacaa aactgaagaa gtattcgact ttaaacttat tagccccaca
    11161 aaagccgaaa aacttatcaa aaaggccagt ccgcgccgct ggtcaaaagt cgaggcactg
    11221 ataacacgag ctgatggtaa acccaccatc gttcccgagt cagacccacg ccccgcactc
    11281 aatatcaacc ctgtaaatga tttcgacgac gtatccgacg atacgctaac cgcagacctc
    11341 atctgattta aggaaatccc catgaaactg aagctgaaca atgttcgtct ggccttcccg
    11401 tctctgtttg aagctaaaac tgtaaacggc gaaggcgatc cgcgtttctc cgcagtattt
    11461 ttaatgtctc ccaaacaccc acaactggaa gaaatccgta aagctatgaa gaaggtagcg
    11521 aaggaaaaat ggggggagaa gtgggagtcc atttataacc agctggagaa aaaactcaat
    11581 ctgtgcctgc atgatggtga tgaaaaagca gagtatgaag gctttcccgg caatttcttc
    11641 ctgaacgctg ctaacaaagc gcgcccagct gttcttgatc gcgatcgttc gccactaatt
    11701 caggctgatg gacgtcccta tgcagggtgc tatgtaaacg ccgttatcga tatctgggca
    11761 caggacaata atttcggtaa acgcattaat gcctcactcg gcggagtcca gttcctgcga
    11821 gacggcgatg cattcgctgg cggcggagtg gcaagcgctg acgatttcga cgatatcagc
    11881 gaaggtgctg atgctgaagc actaatttaa agcatcaatg tatcaggtcg ataatatcat
    11941 ttgacctgat acttacttgc atctactttc aataactgta aattatactt atttataact
    12001 tctcgccccg ggaaaagagt ccagaacata aaaaacgtaa acaaacaaaa caacattaca
    12061 acaacgccga gaactatata aacagtaccc ttatttagca acaaacacag aaatattagg
    12121 ccatacccca aataaaaaat agctgcaatt ttagaatgca agcgatatcg tttatattta
    12181 cctttataac gtataaaaaa tctatcatat ttacattgaa tatgaggtgc taagaaccac
    12241 caccgccaag atggcatatc taattcagaa tacgttctta tataaagaat taacggccta
    12301 agtttcaaat taaccatacc caagatgctt tttttgattt ctctttttat ttctgccttc
    12361 ataaactcta actcagaagg atcaatatac tcccgataat tttcaagaat atgtgagaat
    12421 tttgttgttt tcgacaaaaa ccaactaaca tcattcaata atttataaac accgactgct
    12481 gcggttatac ctgggattat gagtccgaca agtgcaataa catcttttat ttccatacaa
    12541 ctaacccttc ggaataaata ccatgtccaa tatactatgg ggcgacctag aaacctattg
    12601 cgaaatccct attacgaacg gcacccatgc gtatgccgaa ggcgttgaag tgatgctttt
    12661 cgcatgggct atcaacgacg ggccagtaaa cgtgtgggat atcactgccg gtggtggtat
    12721 tccacacggc ttatacgaag caatcgcaga ccctgaaacc ctgctttatt tccataactc
    12781 tcacttcgac cgcaccgttc tgcgttatgc aatgccgcga ctggcaccgc cagtcgaacg
    12841 ttggcgcgac acgatggtgc aggcgctggc gcacggtctc ccggggtctc tgggggaact
    12901 ctgcgaagta ctaggcgtcc cgcaagacaa agcgaaggac aaagaaggta aagcgctgat
    12961 ccagctgttc tgtaagccac gcccgaaaaa cagcaaactg cgtcgggcca ccagtaaaac
    13021 ccacccggaa gaatggcggc gctttgttgc ttacgccgga ctggatatcg aggcaatgcg
    13081 cgaagtctat aaacgtctgc cgaagtggaa ttatcagggg acagagctgg cgctctggca
    13141 tcgtgatcag cagatcaatg accggggcgt ctgcatggac gtggaactcg cacgcgctgc
    13201 gatcgacgcg gtagaccaag aacaaaagcg cctggcaaag cgtacacagg aaatgactga
    13261 tggcgaagtg caggcagcca cacaacgaga cgcgttgatt aagcacattg ttgaatccta
    13321 cggtgtggag ctaccagaca tgcaacgcag tactctggaa cgtcgtattg ccgaccccga
    13381 tttaccatct gccgtgaaag aactgctggc tatccgcctg caggccagta ctaccagcac
    13441 cagtaaatac aaggcactga tgaaaggcgt aagccacgac ggacgcttac gcggtacact
    13501 acagttctgc ggggcgtcac gtaccggtcg ttgggccgga cggctattcc agccccagaa
    13561 ccttccccgc ccttcactaa aacaggaaca aatagacgaa ggcatcgaag cactgaaagc
    13621 cggatgtgca gacctgctgt ttgacaatat catggaacta accagttcag cgttacgtgg
    13681 ctgcattatc gcgccaacag gcaaaaagct ggtggtaagt gacttgtcga acattgaagg
    13741 ccgtatgctg gcatggctgg cgggagaaaa atggaaactg aatgcattca gagagtacga
    13801 cgccggaacg ggtccggact tatataaact ggcgtatgca aaagctttcg atattgcacc
    13861 agatgatgtt gataaacaca tgcgtcagat cggtaaagtc atggaactcg gtctgggtta
    13921 tggtggtggt gtgtcggcct tcatcacttt tgcactggtt tacggtctcg atctcgacga
    13981 gctggcgaac gccgcactac caaacattcc ccgcgatgtt atccgcgagg cgaaaagctg
    14041 gtacgacgaa tcggttaaac gcaagtcaac ctacgggctt tctgaacggg tatttatcgc
    14101 ctgcgactca cttaaacgtc tctggcgcag ggcgcacccg gcgacctgcg atttctggta
    14161 cgagctggag cgcactgtcc gcacagcaat cgccacaccg caaaaaacat tgtattgcgg
    14221 ttatcttaaa atccgccgcg atggcgcgtg gctgcgcata cagctaccat ccggacgcgc
    14281 tgtatgctac ccgtctccgg ttatcgaaca agggaatatc acctacatgg gtgttaactc
    14341 ttattcgcgt aaatggcaac gactcaaaac ctacggcgga aagctggtgg agaacgtcac
    14401 ccaggcggcc gcccgcgacg ttctggccgg aaacatgccg cttatcgaag atgccggtta
    14461 cagcattgtg ctgacggtac acgacgaggt gatcaccgaa tcacctgaca cagaagattt
    14521 caacgataaa gcgctttccg cgcttctctc cactaacccc gaatgggcgc ccgatatccc
    14581 actgaacgct ggcggttttg aggcgtacca ctaccgtaag gattaactcc tatgccatct
    14641 aaataccggg ataatccact ttattacagg gctgcaaggg aggctttgcg acttgaacaa
    14701 ttcggcgaat atgaccgggc agcgaaggcc tgggcaaaag ccaaccgcga atcacatcac
    14761 gaactgaatc aggaatggag tgaacgcaga tctgattttt gcctgatgca gaatatgcgt
    14821 gaaaagcgta aggcggtcga agaatgatcg tttacgttgc cgggccgatg agcggttacg
    14881 agcaatttaa ccgcccggct tttcattccg cagcaaagag gctaacggac aagggatatg
    14941 ttgtgcttaa ccccgcaact ttacccaatg gtttgacaca ggctcagtat atggacatct
    15001 gcctcgcaat gcttcgctgt gccgatacga tttatatgct caaagagtgg gagtactctg
    15061 ctggcgcacg tgcagaaaac gcgttggcag agaaactgga attgaatgtg ctttttgaag
    15121 agtgggattc agatggccta tgaacgtgaa agccttatcg aaaagcacct cgtcgctgaa
    15181 gtgaaaaaag ctggcggagt ggcctttaag tttgtatctc ccggtcaccg ctcggtaccg
    15241 gatcgcattg tcctgctacc cggtagtcgt atcgttttcg ttgaatgtaa atcacccggc
    15301 aagccaccac ggcctgacca attgcgcgaa catgaacgtc tgcggaaact gggctttacc
    15361 gtagtggtgc tggatagcaa aaatctggag gggatattgt gagtaaacgt gacgacccac
    15421 aattgcgggt acgcatcccg caagacttga aagacgcact ggaaaaaatt tccagagaaa
    15481 acgacagaac actaaccgcc gaaatcaccc ggcggttacg taaaagttta gaggatgaat
    15541 ccactattca ttctcaggat ttagcaactt cttaagtttt tccatcgctt caataagctc
    15601 gtatttgttt tcataaaccc attcggcctg atactctcga tggtacgttg ttttaagttt
    15661 ctcgagaaga tccctgagta tgagaatctc atcagcagct tcctcaaacc ccctagatgt
    15721 atgaagtaat ttgtcctgaa gaatagtaga ttccaaccgg ctaacgatct cagaggtcag
    15781 tgttctctta ttagcccgtg ctttcttctc caaagcctct tttagcaatt ctggtatgcg
    15841 taccctcaac tgcgggtcgt ctcttttgct catatatgat cccttgaaag tgtgaggaca
    15901 acatgcctca caaacatgtt gacttcaagc ctcattgtga gtactaataa ataaagtcct
    15961 cacaatgagt acaaaacaag gctaaagcta tggctattca gatcacacag ttacatcagg
    16021 cagatatttt cgggtatatc gcagacatgc tggaaacagc gagacttctc agttcgctgg
    16081 agaaaggaga gcagttagct tttgaattga tttattttgc gcaacaggcc gcaagggaag
    16141 cagcaaacaa gccgtgggat gaataaacaa aacacgctga tagttgcagc taccagcgca
    16201 tcttttacta aatggagaat acagcatgaa tactttaaca ctgataaacg ataccaagca
    16261 accaatgatg ggcagccgga aaattgcaga agtcactggc aaacagcata agcatgttgt
    16321 acgggatatt ctggctatgc ttgaacagtt acaaatctat agtccagatt tggatgatga
    16381 tgattttaaa ggatttatta tcaagcgcaa agaatacaag ggacgtgatg tcatcgacga
    16441 gatttggctt gatgaaaatc tttccatgac tctcgtcacc ggttacgacg caaaacgccg
    16501 gctggcactc atcgaacagt ggcaggcgat gaagattgaa ttagagcaac ctcgtccggc
    16561 atcattaccg gtgccagctc ctgcaattca ggtaaacgac aacatcgttc agtttgcccg
    16621 tgtcgttgca gaagctacgg catccgccac gatgaaagca attgtcgaag tgatggctcc
    16681 ccggcaagtt caaaacgtaa cgctttcaac tccgggacat gaaaccaact gggtagaaga
    16741 cttaaaacaa caagtcagag agtcacaacc acacagtaac ggtgaatatg tccccgtcag
    16801 caaagcagca tggaaaacag gtttatctga ttcaacatgc cggaaattga tcggcttcgc
    16861 caggataccg gtacgttcag acaccggtgt tcggggattg ctcgttaagc tcccagcgat
    16921 cgaacaagca gctcaaaagc tgttaaacga atcgacaccg ccgaaaggta accgcaagcg
    16981 ctggagacat ccgcagtttg gcaacttcac ctactacacc gatctaatct gacattattg
    17041 cgcgggatta cccgcgccct actggctaaa acactatggg acaaattttc accccccgcc
    17101 cgtatcaaga cctgatcatc aatcaccaaa tcgaaaccct gcgcggcaac atctgggcag
    17161 ggatggggat ggggatgggt aaaactgtag caacactcac tacgctggaa gatcttttca
    17221 tggcaggggc agaaacacgc cccgcgctgg tcctcgcgcc gctacgtgtt gcagcaaaca
    17281 cctggccgga tgaagcaatg aagtgggggc atttgcgcaa tatcgaggtg cagccgattg
    17341 ttggtaacgc caaagcgcgc tctgcagcgc tggcgaacag caacgcgagc gttttcacca
    17401 tcaactacga taaccttgtc tggctggttg aggaattggg agaacgatgg ccgttcggta
    17461 ctgtcattcc agatgaaagc acccggctaa aatccttccg gctgcgaggt ggtggtaagc
    17521 gcgcggcggc gctgggcaaa gtggcgcata agtatgtccg gcgctggata aatctcaccg
    17581 gtacgccagc accgaacggc ctggtagatt tgtggggaca atcgtggttt gtggaccaag
    17641 ggcaacgtct cgggcgcact tacggcgcgt ttacctcacg ctggttcaac tcgatacagt
    17701 ttccgggaca gagctggact aaactggagc cttttgctca ctcacagggt gaaatacagc
    17761 gagcattagc cgatgtgacc ctctcgctgg atgcggccga ctggttcgat atcaaagacc
    17821 ccatccataa cgtaatccgc gtggatatgc cgccgaaggc ccgtcagcag tatcgtgaaa
    17881 tggaaaagga aatgttcctc gagctgaatg gcgaaggcat cgaagcaccg aacgccgcgg
    17941 caaagacact gaagtgtctg caaatcgcca gtggcgcagt atacacagac gacaccggaa
    18001 gttggtcaga actgcatgac accaaactac aggcgctgga cagcatactg accgaagcag
    18061 ctggcgcacc tgtgctggtt gcttatcact ggaaacacga tcttgaacgc ttgcttaaag
    18121 cattccctcg cggtcgtcac ctcgaccagg atccacagac actgcgcgac tggaattccg
    18181 gaaagattcc tgttctcttt gcacacccag ccagcgcggg ccacggcctg aacatgcagg
    18241 acggcgggaa tatactggta tttttctcac actggtggga cctggagcag tatcagcaaa
    18301 ttattgaacg tatcggcccc accaggcaga tacaggccgg acacaatcgt ccggtattta
    18361 ttcaccacat tattgctgcc gacactatgg atgaaatggt gatggagcgg cgcaactcaa
    18421 aacgaacagt gcaggacatc ctgctcgatg ccatgaaaaa gagaggtata gcatgagcga
    18481 gaaacccgac gatttactca ccccggatga agtatgccaa aagttaggta ttacacagaa
    18541 aacgctatgt gagtggaata ttaagcatcg tcatcgggct atcctggcac caattcgttt
    18601 cagtgcaaaa gtagttcgtt atgagcgccg aaatgtcgac gcttttattc aaaaatgtcg
    18661 cagccagtat taacctcgcc gccgtagcaa tgccacctgc gcaagtatgc tccgctcgtg
    18721 agcctcgaaa gcttcgcgct tcaacgcaat ctcttcctgt aaaatctcat cagaaaagtc
    18781 gtaatgttct gccatcgggt catctgactt actggagtgg tgaaggcaaa ggaggctgat
    18841 ttcccttcgg tctgatcggg aatagcctct ttccttcatc aaggcaataa cattgctctt
    18901 aaggaattta cggcacatcg tattaaatgc accgtctttc cctttaacag tcccatcatg
    18961 ttttattcct tttacagccc cgtccgggct gtatgttttc accagcttat ccagtgatcg
    19021 ttttgaaaat ggctgcattg gatcacgtgg ctgcaaaaat acataatccc tgttgcactc
    19081 aggaactgaa tcacgccagg ctttctgctc gtcgataatc cgccggatct caggcgttat
    19141 cggcaggcgg aaagcctttt gtgttttcat agcccctcgc atgccgataa ccccttccgg
    19201 ataaacaatt tcaccagcct cctcgtgaac gtagtcccag cgcaggttat ggacattaat
    19261 cgggcgaacg ccggtgatga tcatgaagcg aacagcattc ttctggtgta cagaggtgca
    19321 ggcggcaaca ttgagccaga gccgggcgat tgactcaata tcggtaaaaa gccgtgtggg
    19381 ggtgggtttc tgtacgcggg aggaaacata atcatctggc agactggcgg caacattgcg
    19441 gccgttgcaa agagtaggtg cgcagaactt ccagaaccga cggagctcgg caaacaactc
    19501 tagggcgtta ttgttcgagc gagtggcgat ccactcgtcc agtacttcca ccagccgatt
    19561 gtatgttacg tcgctgaaca cctctcgctc gccgaacgtt gctttaatcc ggtcgatacg
    19621 caccccgtag gttgtgaaac tgtccgggct cagcttctgc cgtgctactt tagctttgag
    19681 gtcatcccgg tacatttcca gcgctgcatg tacggactct gcccgcagtc caccgtcagc
    19741 catacctagt gctttttcgc gcgccagttg aatagcgagc tccggccact cgccaagctt
    19801 tttacctttg aggcccatct tttttggaaa ctcggcgtaa aatgtaacct taccggcttt
    19861 gctgaaatcg atacggagat agttctcttt ttcgtatttg gaacggcgag ccacgccgga
    19921 agcagcgagg atgattttgg cagcagcaac acagattttc atgtgtgcgc tggtataggg
    19981 gggtttacag gcgtcccatt ttccagatgc ggctaaaaca tcgtcattat tggggctatc
    20041 cggattatgt gttacagtgc gcggcattct caatccttat ctgcgtaggc gcagaaaaca
    20101 agctcacaca tacaagtctt ttctacggga caaaatgcaa tgtgttgcgg ttttgtgttg
    20161 ctggactgag tttatcaagg ttaaatacac tggatcaaca tacagtaagt taatgacagt
    20221 aaagcataca aaatcgatac aacttactga ttttaaaata atttaacggt aatccattga
    20281 aatgtcttta ctaattacta aacgctgtat taattgtgat atgtgtgaac ccgaatgccc
    20341 gaatgaggcg atttcaatgg gagatcatat ctacgagatt aacagcgata agtgtaccga
    20401 atgcgtaggg cactacgaga caccaacctg ccagaaggtg tgcccgatcc ccaatactat
    20461 tgtgaaagat ccggcgcatg tcgagacaga agaacagttg tgggataaat ttgtgctgat
    20521 gcaccacgcg gataaaattt aatattctac tctggaagta gagtattaat tatattactg
    20581 ggaagcctta acgccattat atttatttaa ttgatgacat tagcataatc attcactaag
    20641 ttaatttata tagtatctgc caagacactt atttatagtt attaaaggcg cgtccgattg
    20701 gttcaccgga cgcaccttaa gtacgtttcc ttgtgttata agaacagaag gatcagctgt
    20761 aaaacagcaa tgatgatttt gatgacccgt ttaatcaggt atcggcaatc agtcattcgt
    20821 ttttccttaa acaaggaatg cagccgttca gattagccct tacatctccc caaaactgaa
    20881 cgtgcgagtt attgagggtg catgctgcac tccacaccag agctttgacg acaccactcg
    20941 tttcaatggg ggaattctgt ggcatggtgt aaagcacagc aaaatcttca ataacgaagc
    21001 caattttaat gtacttacga attggcggtc acgttaatct ttccatcaat attgctttct
    21061 tcgtaaaggc tcgagttttt atgctaaaga ttgcaagttg cttgtaaaag ataagtacac
    21121 tgatccataa tcgctgttgt tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa
    21181 acccccgttc cttaccagtt cgggggtttt actttttaaa gagaacggta ttattttaac
    21241 tttcaataat taccgtggca caagcatagt ggcgttcatc tgccagcgtt acatgcatat
    21301 ttacaacgcc gagcttttcc gccagtttta atgcctcacc ccatagccgt agccgtggtt
    21361 tgccgagctc atcattgaat acttcaaatt gattaaacgc cagaccattg cggatcccgg
    21421 tgccaaacgc ttttgctgcg gcttctttca cagcaaaacg cttcgccaga aaacgcaccg
    21481 gctggtggtg cgttttccag attgcccatt cgttatcgct taatacgcgg cgtgccaggc
    21541 gctcaccgga tcgggcgatc actgcttcta tgcgagcgat ctctacgata tccgtgccta
    21601 aacctaatat tgccattagc cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc
    21661 atctttcagt ccggtcatca ctgcacgacc aataatggca tgaccgatat tcagttcatg
    21721 catctcaggg atggcggcaa tagctttcac gttgtgatag gtcagaccgt gaccagcgtt
    21781 aactttcaga ccgagactag cggcaaaggt cgcggctttg gcgatacgcg ccagctcttg
    21841 cgcctgttcg gcgtcagttt tggcatcagc atagcaaccg gtgtggatct cgataaacgg
    21901 tgcgccaacc tctgccgcag ctttgatctg ctcttcatcg gcgtcgataa agagagaaac
    21961 ctggatcccg gcatctgcca gacgtttgca ggcatcgcgc attttgtcac gctgccctgc
    22021 gacgtccagg ccgccttcgg ttgttacttc ctgacgcttt tccggtacca gacagcaaaa
    22081 atgtggcttc gtctcaacgg cgatcgccag catctcttcg gtcaccgcca tctccagatt
    22141 catgcgggta tccagtgtct gacgcaggat gcgcacatca cggtcagtaa tgtggcggcg
    22201 atcttcacgt aaatgcacgg taatgccgtc cgctcccgcc tgctcagcaa taaacgccgc
    22261 ctgcaccgga tccgggtaag cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat
    22321 gttgacgcct aacagtaatt cagccatgac aatcctcatc attcataatg tgttttcacc
    22381 gttcgcttag gcataaactg ccggaacagt tccctgctct ttaatggttt accgccaaga
    22441 tacggcttaa gcgccatgcg ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga
    22501 aattcccgcg cgtttaacgc ttttaactgc cttccggtga acgttttatt gtcgataacg
    22561 acgcttgcga taaacccttt ttcttcgcga taacgatacg tcatggtgtc atctaccggc
    22621 tcgccgctac ccgcacaatg ggtaaaattg acgccatagc ccagatgccc gagcagtgcc
    22681 agttcaaagc ggcgcagcgc gggttctggc gtaccagtgg cacctgcaag agactgaatg
    22741 cagtgcaagt aatcgaaaaa gagttcagag aagcgcgtct cgtattccag tacgcgggag
    22801 agaagttcgt tgatgtacag accgctgtaa agcgtgatac cgcttaatgg cagcgccagc
    22861 gagacagctt cagcactgcg cagcgttttg acttcgccac gcccgccgaa acgtagcaag
    22921 agaggggtga aaggctgtaa tgcacctttc agagtagagc gtttagagcg tgcgcctttg
    22981 gcaaccagac gcacgcgacc cgattcctcc gtgaagacgt ccagcatcag gctggtttcg
    23041 ctccacgggc gactatgcag gacaaatgcg cgctgccagc cttccatcgg agtgactctt
    23101 aaagatcgtc aacgtaaccg agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt
    23161 tcacttttac ccacagctca aggtggacag gcgcttcgaa catttcctgc atgtctttac
    23221 gcgcttcaat cccgatggtt ttgatcttgg cccctttgtt gccaataacc atcttcttct
    23281 gcccttcacg ctcaacgaga atcaaaccat tgatgtcata accaccgcgt tcgttagaga
    23341 cgaaacgttc gatctccacg gtcacggagt atggcagttc agcgccgagg aaacgcatca
    23401 gtttttcgcg gatgatttca gacgccataa aacgctgtga gcgatcggtg atgtaatctt
    23461 ccgggaagtg atgggtcgct tcagggaggt gcttacgcac gattgctgcg atagtgtcga
    23521 cattcagccc ggtttcagca gagatcggca cgatatcgag gaagttcatc tgacttgcca
    23581 ggaactgtaa gtgcggcagc agatcggctt tttcctgcac gttatccact ttgttcaccg
    23641 cgaggatcac cggcgctttg ccatcgcgca gtttgttgag caccatttcg tcgtccggcg
    23701 tccagcgggt gccttcaacg acaaaaatca ccagctcaac gtcgccaata gagctgctcg
    23761 ccgctttgtt catcaggcgg ttaatagcgc gtttttcttc catatgcagg ccaggggtat
    23821 caacgtagat cgcctgatac gcgccttcag tatggatccc cacaatgcgg tgacgagttg
    23881 tttgcgcttt gcgggaggtg atggagatct tctgccccag cagtttgttc aacaatgtgg
    23941 atttgccaac gttcggacgt ccgacgatgg caataaatcc gcagtaactt ttatcgatgc
    24001 tcattccagc tccagttttt tcaacgcctg ttcggcggca gcctgctcag ccttacgacg
    24061 gcttgaacct gtgccaacca ccggttcact caggccgctg acctggcagt ggatagtaaa
    24121 ttcctgatcg tgcgcttcgc cacgtacctg gactaccaga taagtcggca gcggcagatg
    24181 gcgaccctgc aaatattctt gcaagcgcgt tttcggatct ttttgtttat cgcctgggct
    24241 aatttcgtcc aaacgagttt gataccagtt gaggattaat ttctcgacgg tttgaatatc
    24301 actgtcgagg aatacgccac caattaatgc ttcgacggtg tcggcgagaa ttgactcacg
    24361 acgaaatcca ccgcttttaa gttcacctgg ccctaaacgt aagcactcgc ctaactcaaa
    24421 ttcgcgcgcc agttccgcca gcgtattgcc acggaccagc gtggcgcgca tccggctcat
    24481 atcgccttca tccacacgag ggaaacggtg ataaagcgca ttggcgataa cgtagctcag
    24541 aatagagtcg cctaaaaatt ctaaacgctc gttatgttta ctgctggcac tacgatgagt
    24601 taatgcctgc tgcaacagtt cctgatgatt aaaagtgtag cccagcttcc gttgaagccg
    24661 attaattacg atggggttca tgcgatacca ataaataaat gcgtcaacaa ttcagcacac
    24721 gaaacagacc tgatatacat ggctctgcta actgcttcgc tgcagtttca gtttaccggt
    24781 atatggggac caacgctgtt tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt
    24841 cgggggaata ttctatacac aacgacgggg gatgtcgtta gccacgggag atttatctca
    24901 taaataattc acgttgtcgc cataacggcg acaacgtgaa cgaagatgag ctattaatgg
    24961 atgccgccaa tgcgacttaa gcgcacacca gtcggccatt cgccttcttg cttatcgaag
    25021 ctcatccaga tagccgttgc ccgaccgacc agattcgctt caggcacaaa cccccaataa
    25081 cggctgtccg cgctgttgtc gcggttgtcg cccatcatga agtattgtcc cggaggaaca
    25141 atccaggttg ccagttgttg ccctggctgc tggtaataca tccccacctg atcctgcgca
    25201 atcggcactg tcagaattcg gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga
    25261 cgaattccat tttctttggt ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc
    25321 ccaccattac ggcgtgagaa ggtctgaacg aaatcgctcg gttccacgtt tgagtaggtg
    25381 accggcagcg cgttttcaca cgcctggccg gaactgcatc ccggttgaat cgtcagctct
    25441 ttcgagaccg gatcataagt aactttatcg cccggtaaac ccaccgcgcg cttgatgtaa
    25501 tcaagctttg gatcttccgg atatttaaag accacgatat ctccgcgttt cggatgaccg
    25561 gtttcgatca gcgttttctg gtagatagga tctttaatgc cataagcaaa cttttctacc
    25621 agaataaaat caccgattaa cagagtcggc atcatcgaac ctgacgggat ctggaacggt
    25681 tcataaataa acgaacgcac aatcaatacg atagccagta ccggaaaaac agaagcaccg
    25741 gtttccagcc agccaggctt cggcgcaacc tttttcaacg ttgctttatc cagtgagtca
    25801 ccggcagccg cctgcgccgc tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta
    25861 tccacgcacc ataaaatgcc cgtcaccagt gtggcaatca ccagaatcag ggcaaacata
    25921 ttcgccatgc caactcctaa gggttatttg ttgtctttgc cgacgtgcag aatggcgagg
    25981 aacgcttcct gcggcagctc gacgttaccg atctgcttca tgcgtttctt accttctttc
    26041 tgcttctgca gcagcttttt cttacggctg atatcgccgc cataacattt agccagtacg
    26101 tttttacgca gctgtttcac ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga
    26161 atggcgatat caaactgctg gcgtgggatc agatctttca tcttctccac caactcgcga
    26221 ccgcggtttt gcgaattatc acggtgggtg atcagcgcca acgcatcaac acgttcaccg
    26281 ttgattaata cgtctacacg taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc
    26341 agagacgcat aaccacgcga ggtagatttc aggcgatcga agaagtcgag caccacttcc
    26401 gccatcggga tctcgtacgt cagcgccacc tgattaccgt ggtaaaccat attggtctgc
    26461 acgccgcgtt tttctacgca cagcgtaata acgttgccga gatatgcctg cggcagcagc
    26521 atgtgacatt cggcaatcgg ctcgcgcagt tcgtagatgt tatttaccgc tggcagcttg
    26581 gatggactgt cgacgtaaat aacttctctt gacgtggttt caacttcata cactacggtc
    26641 ggcgcagtgg tgatcagatc cagatcgtat tcacgttcca gacgttcctg gatgatctcc
    26701 atgtgcagca ggccgaggaa gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc
    26761 ggctcataga acagtgaagc atcgttcagg ctgagtttac ccagcgcgtc acggaaggct
    26821 tcatagtcgt cggaacttac cgggaacaga ccggcgtata cctgcggttt gactttctta
    26881 aagccaggca gcgccttttc tgccggatta cgcgccagcg ttaaggtatc gccgactgga
    26941 gcgccgtgga tatctttaat cgcacatacc agccagccta cttcgccaca tttcagttca
    27001 gtgcggtcaa cctgtttcgg cgtgaagatg cccagacggt cggcgttata ggtctgcccg
    27061 gtactcatga ctttcacttt gtcgccctta cgcagggtgc cgtttttaat acggataagt
    27121 gaaacaacgc ccaggtagtt gtcgaaccat gagtcgataa ttagtgcctg caacgggcct
    27181 tccggatcgc cttccggcgg cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg
    27241 ccaacgccgg ttttcgctga acagcgcacc gcgtcagtgg cgtcgatgcc gacgatatct
    27301 tcaatttctt ccgccacgcg ttcaggatcg gctgccggca ggtcaatctt gttcagtact
    27361 ggcaccactt cgagatccat ttccatggcg gtgtagcagt ttgccagggt ttgcgcttcc
    27421 acgccctgcc cggcgtcgac caccagcaat gcaccttcac aggcagccag cgaacgggaa
    27481 acttcatagg agaagtctac gtggcccggg gtgtcgataa agttaagctg ataggtttcg
    27541 ccgtcagacg ctttgtagtc cagcgtcacg ctttgcgctt tgatggtaat gccacgctca
    27601 cgctcaagat ccatggaatc gagaacctgc gcctccattt cacggtcaga caggccaccg
    27661 cagatctgga taatacggtc agacagcgtc gatttaccgt ggtcaatgtg agctatgatc
    27721 gaaaagttac gtatattctt cataaagtat gattattgtg ccttaatgcc cggttaacca
    27781 ggcttttaga agtcgctgtt ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta
    27841 cactacaaca ttgaggcgag gaaatgttca taccgtatgg attgtggtat ctggaaacgt
    27901 cctcgcattt gttatgcaaa atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg
    27961 aagatgtttc aaatcgcaca aggccaggcg gcaatgccac gcttaagatg atcggttgcc
    28021 attctgcccg ggcagcaaac ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac
    28081 caccgaggat cgcaccacat aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg
    28141 aagcgataag gaataatccc accagcggcg acatataaac cagtaatgcg gagctaagca
    28201 ggctgccttc ggcgatcccc aattccactt tttgccccgg cactaacggt tcatcacagg
    28261 gtacgacaat ggtatgcgtg gtttgcgggc caagtttatt caacacgcgg ctaccgcaac
    28321 cggcgcgtga agcgcagctg ctgcatgaag ctttaacatc acaactgacc agcgcctgcc
    28381 cgttttgcca ggagacgacg gtagcccact ctttgatcat tgcgctgccc cgaacttaat
    28441 attctcggca atgcgtttcg ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc
    28501 gttatcacgt acgcttgtac tgacggttct gcgtccggtg cgcaacatct gatcggtgct
    28561 cgatggcgta gcgcggttaa cgtttaccga gaagctgaat aatccgtcgg aatagagacg
    28621 tgattcgata ggcatgttgt ccatcgtcgg tagcggacgt cgactactgg aaacttcgct
    28681 aaaaccctgt ggcaaccagg ttggcgtcca gctgaattta gctttttcac ctacaggaac
    28741 agaaagcaac ggcggcaaat ttgccttcgc cagcgtctgc atactgctac tgatatcctg
    28801 attgacgtta aaagcaatca cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag
    28861 atcaaccctc atcggtaatt tcgattcgat gtccatccac acgatgtagc tgtagcgtgt
    28921 accatctcgg gcaaccacgc gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc
    28981 gacggagata aagtcgtaat aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg
    29041 cagagaatca acaatgtaat cgccattaag cgtgaacggt tcaagtcccg gttcaaaata
    29101 gctgatttca ttgccgcgct gtaccacttc ccggcgcggg ccatccattt gcaacaattg
    29161 tgcaagagga cggttatcga ggcgtgcatg tcgataacgc agagactcaa caccctgttt
    29221 attgatgctg atgaatgaca gctcgtaatt cagtgactga ctggccaggt tcatctgctg
    29281 taataacgcc ccggacgcgg gagtggccga ggcgttagca gagaataaca ggctacctgt
    29341 cactaatgac atggcaaacc aaagttgctt cattactgcg attgcgttcc taaagtttga
    29401 attcctggca cctgtacagc ggcttgctgg gtttgcgcct gctcaaactg aagctgttca
    29461 gagtggagtc ggcgttgcag ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc
    29521 tcctgtacct gctgctgttg accattgttt gcggtcgctt cagaaggtac tcccaggctt
    29581 accgggctgg ctttacccat catcggcagt gtattaaata ccggcgtttc gggctgctgg
    29641 gacgtttcag attgtccatt atagtgctgg acgccaacga taactgcaag cgatacgcat
    29701 gctgctacgc ccatttgggt aagctgtgcc gcccacggac gtactttctg ccagaatggc
    29761 attttctgcc attgatgcgg cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt
    29821 actggctctt cttcaatggc ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg
    29881 ggagtatcac cccgcattga gtcacggatt aagtgatagc tttcccaggt tttctgcatt
    29941 tctgggttat gagccagttc gttaagcagc tcactatcca gcgtttcgcc atccattaaa
    30001 gcggaaagtt gttctttctg catgcctaat acccttatcc agtatcccgc tatcgtcaac
    30061 gcctgataag cggttgaact ttgttatcaa tagcttccct cgctcggaag atacgtgaac
    30121 gcaccgtacc taccggacaa tccatgatag cggctatctc ttcatagctc aggccatcca
    30181 gctcccgcaa ggttattgcc atgcgcaaat cttccgggag ggactcaata gttcggaaaa
    30241 ctatctgtct cagttcttct gacaacatta agttctcagg gttcgaaatt tctttcaacg
    30301 cgccgccact ttcgaagttt tcagcttcaa tggcatccac atcactggaa ggtggacgac
    30361 gcccctgagc aaccaggtaa tttttcgctg tatttacagc aatccgatac agccatgtat
    30421 aaaaagcgct atctccccgg aacgaatcca gcgcacgata ggctttaata aaagcttctt
    30481 gtaccacatc gggaacatca cccgacggca catagcggga aaccagactc gccactttat
    30541 gctgatagcg cactaccagt aggttaaagg ctttctgatc tcccttctgg acccgttcaa
    30601 ccaggacctg gtccgttaac tgctcgctca tccgaggtaa agtctcccca aaccaaattt
    30661 ccacgcgcta tcgaaacgcc actccattag ctgcaatttg agcaagcaaa gggttagagt
    30721 gtctcgtttt tgtaaagttc cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat
    30781 cattatctat cattataagt ctacagaatc tgaacatcgc attatctgtg tagaaatgcc
    30841 catttaactg cctgaagagt aacccaacgg cctttttatt tcaccaccta atcctccacc
    30901 agccagtaac ttctcttttt ctcgccgccc tgcgtcagcg tgtttagcca ctgtaacaaa
    30961 tattaaaata gcaggtgttt attcgcacaa catgatgcta tgctgaccaa acggtgttta
    31021 gtaaattaaa caaagaaaat gaatactctc ccagaacatt catgtgacgt gttgattatc
    31081 ggtagcggcg cagccggact ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc
    31141 gttctaagta aaggcccggt aacggaaggt tcaacatttt atgcccaggg cggtattgcc
    31201 gccgtgtttg atgaaactga cagcattgac tcgcatgtgg aagacacatt gattgccggg
    31261 gctggtattt gcgatcgcca tgcagttgaa tttgtcgcca gcaatgcacg atcctgtgtg
    31321 caatggttaa ttgaccaagg ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa
    31381 agctaccatc tgacccgtga aggtggacat agtcaccgtc gtattcttca tgccgccgac
    31441 gccaccggta gagaagtaca atccacgctg gtgagcaagg cgcagaacca tccgaatatt
    31501 cgtgtgctgg agcgcagcaa cgcagttgat ctgattgttt ctgacaaaat tggcctcccg
    31561 ggcacgcgac gggttgttgg cgcgtgggta tggaaccgaa ataaagaaac ggtggaaacc
    31621 tgccacgcaa aagcagtggt actggcaacc ggcggtgcgt cgaaggttta tcagtacacc
    31681 accaatccgg atatttcttc tggcgatggc attgctatgg cgtggcgcgc aggctgtcgg
    31741 gttgccaatc tcgaatttaa tcagttccac cctaccgcac tgtatcaccc acaggcacgc
    31801 aatttcctgt taacggaagc actgcgcggc gaaggcgctt atctcaagcg cccggatggc
    31861 acgcgtttta tgcccgattt tgatgagcgc ggcgaactgg ccccgcgcga tattgtcgcc
    31921 cgcgccattg accatgaaat gaaacgcctc ggcgcagatt gtatgttcct cgacatcagc
    31981 cataagcccg ccgattttat tcgccagcat ttcccgatga tttatgaaaa attgctcggg
    32041 ctggggcttg atctcacaca agaaccggta ccgattgtgc ctgctgcaca ttatacctgc
    32101 ggtggtgtaa tggttgatga tcatgggcgt acggacgtcg agggcttgta tgccattggc
    32161 gaggtgagtt ataccggctt acacggcgct aaccgcatgg cctcgaattc attgctggag
    32221 tgtctggtct atggctggtc ggcggcagaa gatatcacca gacgtatgcc tgatgcccac
    32281 ggcgtcagta cgttaccgcc gtgggatgaa agccgcgttg agaaccctga cgaacgggta
    32341 gtaattcagc ataactggca cgagctacgg ctgtttatgt gggattacgt tggcattgtg
    32401 cgcacaacga agcgcctgga acgcgccctg cggcggataa ccatgctcca acaagaaata
    32461 gacgaatatt acgcccattt ccgcgtctca aataatttgc tggagctgcg taatctggta
    32521 caggttgccg agttgattgt tcgctgcgcg atgatgcgca aagaaagccg ggggctgcat
    32581 tttacgctgg attacccgga actgctcacg cattccggtc catcgatcct ttcccccggc
    32641 aatcattaca taaacagata aaaagcctgg gtcagcgccg tatacgcttc ggaatagttc
    32701 tggtctggcc cacgaatgac taagcgatcg ctaaagcatt ctcccgcctg cggggagaat
    32761 gccagcagca cccgatgcgg cagtcgcgct tcgttttccg ccacatccgt ccgcaaacgt
    32821 aaatgccagc ccatgcttaa tgccagttca gtaaaaccat taccaatctg ctctggcagc
    32881 accacgcaga aaaatccctc ttcggtaatg cactccgccg cacaggtcag cagcgatggg
    32941 tgatcaagcg tagtggtata gcgagcctgt tcccgttgag gtgtcgcgca ctctactccc
    33001 tgctgatagt aaggtgggtt actgatgatt aaatcgaagc gtactgtctg ctgtgtgatc
    33061 cactgctgaa tatccgccgt atggacgtta atccgctctg cccacgggga ctggttgata
    33121 ttttcctgcg cctgcgcggc agcttcactt tccagctcaa cggcatcaat tatcacgctg
    33181 tcactggttc gctgcgccag catcagtgcc agcaacccgc tacccgcgcc gatatcaagg
    33241 caacgtttca ccccagccac cggtgcccat gcgcccagca aaatgccatc cgttcccact
    33301 ttcatcgcac agcgatcgtg agcaacaaaa aactgtttaa aagtaaatcc attacgacga
    33361 agcacggatg tagactgtga catgaaaata aaaccttgca ggaaaaacgg cgatagcacc
    33421 gggtgagaac aatacccgag aagcgatatc catacaaaca gatgaagatt gcagccgtaa
    33481 cgtctataat cagcgcccca cacagaggta gaacatgact gtaacgactt tttccgaact
    33541 tgaactcgac gaaagcctgc tggaagccct ccaggataaa ggtttcactc gcccgaccgc
    33601 cattcaggct gccgccattc cgcctgcgct cgatggccgt gatgtactcg gttctgcgcc
    33661 gacaggcacc ggtaaaacgg cggcgtatct gctgccagcg ttgcagcacc tgctcgattt
    33721 cccgcgtaag aaatccggtc cgccgcgtat tttgatcctc accccaactc gcgagctggc
    33781 gatgcaggtg gccgatcatg cccgcgaact ggcgaaacat acgcatctgg atatcgccac
    33841 catcaccggc ggcgtagcct atatgaacca cgcggaagtg ttcagcgaaa atcaggacat
    33901 cgtggtcgcc acgaccggac gtctgctgca atacataaaa gaagagaact tcgattgccg
    33961 cgcggttgaa acgctgatcc tcgacgaagc agaccgtatg ctggatatgg gcttcgctca
    34021 ggatatcgaa catattgctg gcgaaacgcg ctggcgtaaa cagaccctgc tcttttcggc
    34081 aacgctggaa ggcgatgcga ttcaggactt tgccgagcgt ctgctggaag atccggtgga
    34141 ggtttctgcc aatccgtcca cccgtgagcg caaaaaaatt catcagtggt attaccgcgc
    34201 cgatgacctg gaacataaaa ccgcattgct ggtgcatctg ttaaaacagc cggaagcgac
    34261 ccgctcaatt gtgtttgtgc gtaagcgtga acgtgtgcat gagctggcaa actggctgcg
    34321 cgaagcgggc atcaacaact gctatctcga aggtgagatg gtacaaggca agcgtaacga
    34381 agcgatcaag cgtttgaccg aaggtcgcgt aaacgtactg gttgcaaccg atgttgccgc
    34441 gcgcggtatc gacattcctg acgtcagcca cgtctttaac ttcgatatgc cgcgcagtgg
    34501 cgatacttat ttgcaccgta tcggacgtac cgcgcgcgcc ggtcgtaaag gcaccgcaat
    34561 ttcgctggtg gaagcccatg accatttgct gctgggcaag gttggccgtt acattgaaga
    34621 gccgattaaa gctcgcgtta ttgatgagtt acgcccgaaa acgcgtgcgc caagcgagaa
    34681 gcagaccggc aagccatcga agaaagtact ggcaaaacgc gccgagaaga aaaaagctaa
    34741 agagaaagag aaaccgcgtg tgaaaaaacg ccatcgcgac accaaaaata ttggtaagcg
    34801 ccgtaaacca agcggaacgg gcgtgccacc gcaaacgaca gaagagtaat ctcaatgccg
    34861 ggtttaagcc tggtattaaa agtgctggca aacgcaaaa
//
LOCUS       DAHBHC010000054        34357 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHBHC010000054 DAHBHC010000000
VERSION     DAHBHC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.054"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(39..380)
                     /locus_tag="M2A35_003060"
     CDS             complement(39..380)
                     /locus_tag="M2A35_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCB7199328.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(393..1265)
                     /gene="copD"
                     /locus_tag="M2A35_003061"
     CDS             complement(393..1265)
                     /gene="copD"
                     /locus_tag="M2A35_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCB7199329.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMPPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1269..1643)
                     /gene="yobA"
                     /locus_tag="M2A35_003062"
     CDS             complement(1269..1643)
                     /gene="yobA"
                     /locus_tag="M2A35_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCB7199330.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1782..2012
                     /gene="holE"
                     /locus_tag="M2A35_003063"
     CDS             1782..2012
                     /gene="holE"
                     /locus_tag="M2A35_003063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCB7199331.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2114..2770
                     /locus_tag="M2A35_003064"
     CDS             2114..2770
                     /locus_tag="M2A35_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCB7199332.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            2794..3456
                     /gene="exoX"
                     /locus_tag="M2A35_003065"
     CDS             2794..3456
                     /gene="exoX"
                     /locus_tag="M2A35_003065"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCB7199333.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3453..5513)
                     /gene="ptrB"
                     /locus_tag="M2A35_003066"
     CDS             complement(3453..5513)
                     /gene="ptrB"
                     /locus_tag="M2A35_003066"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCB7199334.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5722..6381)
                     /locus_tag="M2A35_003067"
     CDS             complement(5722..6381)
                     /locus_tag="M2A35_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCB7199335.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(6708..7064)
                     /locus_tag="M2A35_003068"
     CDS             complement(6708..7064)
                     /locus_tag="M2A35_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCB7199336.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKVGMAEYQRR"
     gene            complement(7131..7421)
                     /gene="yebG"
                     /locus_tag="M2A35_003069"
     CDS             complement(7131..7421)
                     /gene="yebG"
                     /locus_tag="M2A35_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCB7199337.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            7555..8733
                     /gene="purT"
                     /locus_tag="M2A35_003070"
     CDS             7555..8733
                     /gene="purT"
                     /locus_tag="M2A35_003070"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCB7199338.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(8789..9430)
                     /gene="kdgA"
                     /locus_tag="M2A35_003071"
                     /gene_synonym="eda"
     CDS             complement(8789..9430)
                     /gene="kdgA"
                     /locus_tag="M2A35_003071"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCB7199339.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9467..11278)
                     /gene="edd"
                     /locus_tag="M2A35_003072"
     CDS             complement(9467..11278)
                     /gene="edd"
                     /locus_tag="M2A35_003072"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCB7199340.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11513..12988)
                     /gene="zwf"
                     /locus_tag="M2A35_003073"
     CDS             complement(11513..12988)
                     /gene="zwf"
                     /locus_tag="M2A35_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCB7199341.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13326..14195
                     /gene="hexR"
                     /locus_tag="M2A35_003074"
                     /gene_synonym="yebK"
     CDS             13326..14195
                     /gene="hexR"
                     /locus_tag="M2A35_003074"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCB7199342.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14323..15765
                     /gene="pyk"
                     /locus_tag="M2A35_003075"
     CDS             14323..15765
                     /gene="pyk"
                     /locus_tag="M2A35_003075"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCB7199343.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(15896..16867)
                     /gene="lpxM"
                     /locus_tag="M2A35_003076"
                     /gene_synonym="msbB"
     CDS             complement(15896..16867)
                     /gene="lpxM"
                     /locus_tag="M2A35_003076"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCB7199344.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(16987..18309)
                     /gene="mepM"
                     /locus_tag="M2A35_003077"
     CDS             complement(16987..18309)
                     /gene="mepM"
                     /locus_tag="M2A35_003077"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCB7199345.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNLRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(18325..19257)
                     /gene="znuA"
                     /locus_tag="M2A35_003078"
     CDS             complement(18325..19257)
                     /gene="znuA"
                     /locus_tag="M2A35_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCB7199346.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            19336..20091
                     /gene="znuC"
                     /locus_tag="M2A35_003079"
     CDS             19336..20091
                     /gene="znuC"
                     /locus_tag="M2A35_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCB7199347.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            20088..20873
                     /gene="znuB"
                     /locus_tag="M2A35_003080"
     CDS             20088..20873
                     /gene="znuB"
                     /locus_tag="M2A35_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCB7199348.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(21020..22030)
                     /gene="ruvB"
                     /locus_tag="M2A35_003081"
     CDS             complement(21020..22030)
                     /gene="ruvB"
                     /locus_tag="M2A35_003081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCB7199349.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(22039..22650)
                     /gene="ruvA"
                     /locus_tag="M2A35_003082"
     CDS             complement(22039..22650)
                     /gene="ruvA"
                     /locus_tag="M2A35_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCB7199350.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(22789..22854)
                     /gene="yobI"
                     /locus_tag="M2A35_003083"
     CDS             complement(22789..22854)
                     /gene="yobI"
                     /locus_tag="M2A35_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCB7199351.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            22925..23527
                     /locus_tag="M2A35_003084"
     CDS             22925..23527
                     /locus_tag="M2A35_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCB7199352.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(23529..24050)
                     /gene="ruvC"
                     /locus_tag="M2A35_003085"
     CDS             complement(23529..24050)
                     /gene="ruvC"
                     /locus_tag="M2A35_003085"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCB7199353.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(24085..24825)
                     /locus_tag="M2A35_003086"
     CDS             complement(24085..24825)
                     /locus_tag="M2A35_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCB7199354.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(24854..25306)
                     /gene="nudB"
                     /locus_tag="M2A35_003087"
     CDS             complement(24854..25306)
                     /gene="nudB"
                     /locus_tag="M2A35_003087"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCB7199355.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(25424..27196)
                     /gene="aspS"
                     /locus_tag="M2A35_003088"
     CDS             complement(25424..27196)
                     /gene="aspS"
                     /locus_tag="M2A35_003088"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCB7199356.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            27506..28072
                     /locus_tag="M2A35_003089"
                     /pseudo
     CDS             27506..28072
                     /locus_tag="M2A35_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            28717..32115
                     /locus_tag="M2A35_003090"
     CDS             28717..32115
                     /locus_tag="M2A35_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HCB7199357.1"
                     /translation="MPTISNIHSYNPIYSSKTKAPQTWDTNNYLRKLSEINDDIIFNV
                     QISKITQKQYTFDEVARNEKKYNLNIYDIFFYPQDDIIEYNISRILQHQYSHSPTLRR
                     LVNYYIDNLPETDVNKCQIHIANNYTYNKKEDNISELFIAADEKGNLIVPQKETSEIE
                     ISPEKILLNFFLKHIINPEDLGYSDIDIYTNIIFKELTPQAMAHSSESFQHISVGKEN
                     KLFDSETSEMFTDNIEQVISKGKALQKEFFKSFINNKETESFTIKPDEYSTKKIVLNG
                     LLLTSNMSGTLSNNYNQHNTNKNLADKINIRLSRSLPEEHAAPAGAAAPAIDLFLAQK
                     IEEHLIRKIFPFYPSINRLEQVQEQINYIRGYHELLAFHQTGSWIRNGRNAARDYIVE
                     LIKNHFYKFEIKNSRGYFIFFKDFIHARREHEYRIYEALARDIIVHPQPYHLDYKEPA
                     QEELLISVPENHYMEAIRNKFTNNKPLNFDNMYGKESISISIELDNLKSPGEKNKKNA
                     EHLLKNAQLVIHNLSKNRGDKILCSPNGTKYHRLILNMIVRQCKQHKSTINKRIIDSI
                     SKKIIHNNDFSANIKREISRMNSNRYNPWLIKLAAEKQLSWAETHYQAQRHIQEHMQD
                     CEILNVMDANKLVRDAIISFVHQTNEITEASWMSSEEQHEKKIQALNSFKTKIASMDG
                     GQEFIYSFNKVIQEGLGGLIELSFDIDDTRHHRNLSSLSPASRSGLHLLGSIWNIVMS
                     AVPGFNILSGSSSILNRAIVEKSTDVCGYIQDAIRIGMEAFPVAEAKFKTRASNAKYT
                     GLRFIEEKINKNIIEAPLQRGSSFKVIESIENTDFIYESSSKKILELNLKEDHELFSA
                     TSFDNKNHGYYKRSGDGFYRKQHAYQPLSRETPNKIKYNSKEVELTKEPNSEMYSGTF
                     VDNGKSTIVKFYKSSDGSFYQAEGLKGGGVIRHTDRPYSELKEGDIGYDEELLDITDD
                     SPELEETLPALSQDLYPSEEENVQSLYNKFKNGDVEAGMTEVTLCRGTIASQAENIVS
                     FSTAGGAEIANPNVSPVSEDIAKLQIKSGRIEPEYTTDISVADRFSRGHHLVIVKTKV
                     KYLTRGSISESGWIIPKNAPVEPVGLIDRTFGQPENIQQANASK"
     gene            complement(32499..32765)
                     /locus_tag="M2A35_003091"
                     /pseudo
     CDS             complement(32499..32765)
                     /locus_tag="M2A35_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            32916..33143
                     /locus_tag="M2A35_003092"
                     /pseudo
     CDS             32916..33143
                     /locus_tag="M2A35_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33383..34258)
                     /locus_tag="M2A35_003093"
     CDS             complement(33383..34258)
                     /locus_tag="M2A35_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HCB7199358.1"
                     /translation="MFSIRSLLPISASVSVPTKQSQSIPTTLAGRTIEKAQEKEGLIV
                     FLGMKSVNEYTLNILGQNVSRVTAGKKPYDLLFLNDDTKQDFDKRKMEFTYPEANKNH
                     LQSNNSDVVAAAAISIAATEMKTILPNDLTPGKYNKIYLSGDGSAGLPLLKCGDEFLS
                     PADIVERIVQHNLHEMDDIRLTSCNSADIIKNKDFSPDEIEKSANINNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDID"
BASE COUNT         8917 a         8507 c         8051 g         8882 t
ORIGIN      
        1 ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt ttcagctcgt
       61 tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc aatctgaatc
      121 accgaaatgg gttgggcttt agtcttccca ctggcaactt ccttttgtgc gatatcgttt
      181 aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc cggtcggcaa
      241 gtcagcatca cctcttcgcg attaaacgcc catttgtctt tacccacttc aaaacggctg
      301 acggtaatca cctgcggtgc agccagcgcc gctgcagaac tggtgagtaa cagaaacgcc
      361 agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt cgcaagcagg
      421 ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat ccactcaaac
      481 cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata acgattagcc
      541 agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag ttggccccag
      601 taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac gccgcttgcc
      661 agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc ctgaatggcc
      721 tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag taatccccca
      781 aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat tttcgccgtt
      841 accccttcat tcagtgtcgc atgcccgatt cccgccagca gaataaattg cgcggtagtg
      901 agcataaaca gcaaacgtgg catattccgc ggtggcataa gggcgacgat caacgttacg
      961 agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa aacagcctgc
     1021 caaatgtttg gcgaaaatac atctgcccat cccgttccca tcaacccgcc ctgaacggcg
     1081 agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa acgcagaaaa
     1141 cgcttcgcca gcaagcgacg aatcgttaag ggggccagcc aggcaccgta cattgcgaag
     1201 ccaaagacca gcatcagcga ggtgaaatgg ataaatcgca gcgcgatcca ggtaaacgcc
     1261 agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc gtccaccgaa
     1321 acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag tggaacaatc
     1381 agttgctttt ggtcctgctc atttcgcttc gccggtaatg ttttaatatt ttcgtttttt
     1441 ggccccgtga tttttgcacc actgaatcct gtttcaacac cttccgagaa gtttaaagtg
     1501 attgcctgcg gtgcagctgt cacttgcgcg tttgccgcag gatactgatg cgttaaatgc
     1561 gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag cgcgtagcga
     1621 agggagcgtg cagttgaagt catattatct attccttttt gtaataactt ttttacagag
     1681 cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca tcctggctca
     1741 ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag aatctggcta
     1801 aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc ggggtggcat
     1861 ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa cagcctgaac
     1921 atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg gtcaatctgt
     1981 cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca ctttcttaca
     2041 taacgcctgc taaattatga gtattttcta aaccgcaccc ataatttgca gtcattttga
     2101 aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc ctcgtaaaac
     2161 ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg ccaggcaaca
     2221 atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt cgcgacgtgc
     2281 ccttcctgcc ccacccgatc tctcactgtt agacccgctt tgttatgccg caacgacctg
     2341 gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta ttcgtggaat
     2401 tgcggtgttc gcaccgtggc gaaaaacgcc tgggatctac catcagagtc atggtgcatg
     2461 tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag gtatggatat
     2521 ggacccgacc tgttcactgt ttactacagg gcaatgtctg ggagaacccg acctgctggc
     2581 gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc tgatggcaaa
     2641 tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc gcgccgatcg
     2701 tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg atatcattcc
     2761 attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga tacagaaacc
     2821 tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga cggaaaaatc
     2881 gtcaacccca tgagccacct ggtgcgcccc gatcgtccta ttagtccaca agcgatggcg
     2941 attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga tgtgatccca
     3001 cactattacg gtagtgaatg gtatgtcgcg cataacgcca gctttgaccg ccgcgtactg
     3061 cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt gtggcctggg
     3121 atcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca gacgccgccg
     3181 ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt gcttatcgat
     3241 attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg acgtccgtcg
     3301 ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga cgttgccgaa
     3361 cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag cccggagctg
     3421 cgtttaacac tgaaacatta tctggaaaat acttagtccg caggcgtagc gggtaatgtt
     3481 ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta cgatttaaag
     3541 cgaccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa aagatggtca
     3601 tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata ttgcacctga
     3661 gaatcgtgca aaccggtcgt taccagtaaa tgcggataag cctgtgcggt gacgttgtca
     3721 tatgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt cccccactct
     3781 tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac aacatcaaca
     3841 aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat tgcaacgccc
     3901 atcaacatgc cccccgcact cccgcccatc gcataacaaa gcgaaggaga gccatagccc
     3961 agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt cttcttcaga
     4021 aattttccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac atggacaatg
     4081 gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc ggcatcaata
     4141 cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt gcgaaaatgt
     4201 ttgcgatggt agaccaacga aaccggaact tcgacgccat cacgggcgac tatccacagg
     4261 tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt taatacacga
     4321 cgctcaccgg tatccatatc cagttcaaac aaagtatctg gtgtggtcat ggaagaataa
     4381 ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt cacataggcc
     4441 ggatcatcaa aggcaatacc aatgacttcc cgggtcttgc ggttaatttg gcgcaaactg
     4501 gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt aaacccttcc
     4561 agcataatgt tatcgcgtgg cggaattaac tcttcccact gttgctcatc acgcaggcgg
     4621 gtacggtata agccaaagtt tttgccatgg cggttggaac gcagataaaa ccgatgctgg
     4681 tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa cggctcggca
     4741 tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc caaatgaatg
     4801 actacatagt gcttcgaggt cgttttatgc aggctgacgt aataggtatc gtctttttct
     4861 tcgtagatca gtttatcttg cgatgctggc gtaccgatgg cgtgacgcca gacctgataa
     4921 ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc atttgcccag
     4981 acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttaccagt ttccagatta
     5041 cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc cataatggtg
     5101 ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc tgcgcgctta
     5161 ttggcatcga gcaatgtttc ccactcatcc cactcttcac tgaatgccga ttgacgctgg
     5221 tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc atttttgatg
     5281 tagggcgcag aaacttctcg ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc
     5341 tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg ttgcaggtag
     5401 tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc
     5461 gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag catgttattg
     5521 ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac
     5581 aggggagtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac
     5641 agagaaaatc agcctgttca aatggctgtg cgattctgga tagcccgaaa tagtcaactt
     5701 caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg
     5761 ttcaatgccg tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta
     5821 actgcgttcc ataaaatggt caatatcaat agccgcgcaa ctcaggaaat agatttccag
     5881 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
     5941 tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc
     6001 gattgccgca cgttctttgg catcaatatg accatcactt ttagcggcaa agaccagcgc
     6061 aaggatcaaa cgttctgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
     6121 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
     6181 cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga
     6241 tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta acagtttgcc
     6301 caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag
     6361 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
     6421 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg
     6481 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat caggtgataa
     6541 gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc
     6601 gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg ggcggacaag
     6661 gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
     6721 tattccgcca ttcccacttt gcagtccacg ctgacctggt aatgaatatc ggcactttta
     6781 ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg
     6841 acccaagcca cgggatcggc ctgaccgaca attttttgat catctgccca acgcgccacg
     6901 cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca
     6961 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
     7021 gcagaaacca acaacagccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
     7081 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
     7141 tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg
     7201 gttttcagaa tggtgctcag cacatctttt tgttctgcca gccatagcga aagggcttca
     7261 cgttgctcgt cttccatttg cactggagaa tttgtcagcc aggtgtcaag cagatccgcc
     7321 gtatcaagca ttttatcata agcatcggct tcctttttgc tggtaaacga cattttctct
     7381 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
     7441 ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca
     7501 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
     7561 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
     7621 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
     7681 cgctattccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
     7741 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
     7801 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
     7861 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
     7921 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcgaaag ccttttccgc
     7981 gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc
     8041 aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga atacgctcag
     8101 caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
     8161 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accagtaggt
     8221 catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt
     8281 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt
     8341 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct
     8401 cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga gtttgccctg
     8461 catgtacgtg ccttcctcgg acttccggtt ggcgggatcc gtcagtatgg tcctgcagct
     8521 tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
     8581 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
     8641 cgtctggggg tggcactggc tactgcagag agtgttgttg acgccattga acgcgcgaag
     8701 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaatgc tacaaaaatg
     8761 cccgatcctc gatcgggcat tttgactttt acagcttagc gccttctaca gcttcacgcg
     8821 ccagcttagt aatgcgatcg taatcgcccg cttccagcgc atctgccgga accagccagg
     8881 aaccaccgat gcacagcacg cttttcagcg ccaggtagtc acggtagtta gccggagaaa
     8941 taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt
     9001 tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg tagtccatac
     9061 ccagcatcag ttcggaaaca gtgctgatcc ccggaattag aggaatagtc ccttcggtag
     9121 cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca cccgcttcag
     9181 tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca
     9241 cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca
     9301 gaacgcgcac cccaccagca accaacgctt ttgccatcgg cactgcgtgt tccagttttt
     9361 ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc
     9421 agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac
     9481 aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc
     9541 ctgtccccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg
     9601 cttcgtctac cagcagcgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt
     9661 cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg atagctgacg
     9721 gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc
     9781 ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt
     9841 ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg
     9901 ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct
     9961 caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt aacacttttg
    10021 tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg tcgagtgatt
    10081 tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca agggtataac
    10141 gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca
    10201 gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac
    10261 cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc
    10321 agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg
    10381 aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct
    10441 taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga cgcgcagcgg
    10501 cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact
    10561 gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag aaagtacatg
    10621 ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg tccactttac
    10681 cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg
    10741 gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc atcgtcagac
    10801 ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg ttatgggaca
    10861 gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat tccattccat
    10921 cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg
    10981 cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa ggctggtgcg
    11041 cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca
    11101 aagaggcttt gtcttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca
    11161 actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga taagcagagc
    11221 gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt ggattcataa
    11281 aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca ttcagaggat
    11341 ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat
    11401 aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat
    11461 ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgcaga tattactcaa
    11521 actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg ggtccccagg
    11581 ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt
    11641 ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc tgaataccac
    11701 gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga ttaaaggttt
    11761 ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa
    11821 ctttattcag tacctggata tccacgcctt catcaggttg cagacggata gtcagtttat
    11881 tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga
    11941 ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac
    12001 cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc
    12061 tcttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg gcgaagcccg
    12121 cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg cggcgcagag
    12181 acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gacggcggag
    12241 acatcgcaat catgcaaaga atttgcagca ggtggttctg gatcatgtcg cgcatctgac
    12301 cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct
    12361 caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta gcaaaacgca
    12421 gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg taaacctggc
    12481 actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc agcgacgtcc
    12541 ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc
    12601 ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat
    12661 ttttttgatc cagcatcgcg ccgagacggc tgaatgcagc agtgtcattg acatcgagat
    12721 tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca
    12781 tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc cagtcagcac
    12841 gccctacgcc gataattcgg gtgtccgggt tgagctgacc ggctttttcc agttgataca
    12901 gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac
    12961 aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact
    13021 taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg
    13081 aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa gtgcggcaaa
    13141 actgataaaa aaatcatcgt tttccattgg gtaaaaatct gacactgatc atgttatgaa
    13201 aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg cgtaatatct
    13261 gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt gagtctcttt
    13321 acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc aaatcagagc
    13381 gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg agtattgctg
    13441 ctatggcact ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg
    13501 acacgcgcgg ttttcctgat tttaaacttc atctggcaca gagtctggcg aatggcactc
    13561 cctatgttaa tcgcaatgtc aatgaagatg acagcgttga atcatacaca gggaaaatat
    13621 ttgagtccgc aatggcaacg cttgatcatg tccgtcattc actggataaa tctgccatca
    13681 accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt
    13741 cagccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt ccggtggtgt
    13801 actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg
    13861 tggtgctgat ttctcacact ggaagaacaa aaaatctggt cgagctggcg cagctggctc
    13921 gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc gcccgggaag
    13981 caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg cccatggttt
    14041 ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact ttgcgacgcg
    14101 gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg
    14161 ataagcagtt acttaattta agtgacgatc gctaaaaacg actgtcactg tcctaatctt
    14221 atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat tcggatttca
    14281 tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccag aaggcttcgc
    14341 agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa ccttgaaaaa
    14401 gttatcgcgg caggtgccaa cgttgtacgt atgaactttt ctcacggctc gcctgaagat
    14461 cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg tcatgtggct
    14521 attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt
    14581 ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg tgaaggcgac
    14641 aaagaaaaag tcggtatcga ctacaaaggc ctgcctgctg acgtcgtgcc tggtgacatc
    14701 ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg catgaaagtg
    14761 ttcaccgaag tcaccgtcgg tggtcccctc tccaataata aaggtatcaa caaacttggc
    14821 ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa gactgcggcg
    14881 ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga tctgaactat
    14941 gcccgtcggc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt
    15001 gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc tgacgtggta
    15061 atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt cggcattcag
    15121 aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc gacccagatg
    15181 atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac
    15241 gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc agggcagtat
    15301 ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc
    15361 atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga agctattgcc
    15421 atgtcagcaa tgtacgcagc taaccacctg aaaggcgtta cggcgattat taccatgacc
    15481 gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc aattttcgcc
    15541 atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt tacgccggtg
    15601 cactttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa tctgctgcgc
    15661 gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccagggcga cgtgatgagt
    15721 accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg ttgccggatg
    15781 cggcgaaaac gccacatccg gcctacagtt caatgatagt tcaacagatt tcgaatattc
    15841 tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg cttttttatt
    15901 tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg cgagttttca
    15961 gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc tcgacttctt
    16021 cattcatccg ccgcgcaatc gtatgatcat ccgcctctaa cagatcatcc atcggtgggc
    16081 gcacctgaat cgtcagacgg tgcgtcttgc catcataaat cggaaacagc ggaacaacgc
    16141 gcgcacggca cactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa
    16201 agaaatcaac aaattcgctg tgctcagggc catgatcctg atcgggtaaa taatatcccc
    16261 agtagccctg acgtaccgac tggatgaatg gtttaatacc atcatttctc gcatgcagac
    16321 gaccgccaaa acgacgacgc accgtgttcc agacataatc aaaaaccggg ttgccctgat
    16381 tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca ggaatatcga
    16441 cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc atctcttcga
    16501 tgatctccag cccttgccag tcaacgcgcg gctgaatttt ctccggcccg cgtattgcca
    16561 actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca acaatcgctt
    16621 cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac gcacgacggc
    16681 gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg ggatcacgga
    16741 actttggcgg cgttaaagcg atacccgcca tcgctgctac gcccagccat gctccccagt
    16801 agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta ttttttttcg
    16861 tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag tgtagcggcg
    16921 caacttgccc cgcaccaaat aaaaaaagcc ggtactgact tcgtaccggc tgcgaatgga
    16981 tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca ggaattcgcg
    17041 acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg ggtttacggc
    17101 ctgctggttt atccatactt catagtgcaa atgcggcccg gttgaacgtc cggtattacc
    17161 ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca gaatcttgcg
    17221 caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat aataacctgc
    17281 tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca ctggcgtacc
    17341 ctgcggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgaccggtca ccggattagt
    17401 acgacgcagg ttaaagttag acgagatacg gaactgtttc gccgtcggga atcgcaagaa
    17461 tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcttcag cgcggatagc
    17521 gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt gctcacgttt
    17581 accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt tcagtttgcg
    17641 gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc tggttaaacc
    17701 ggcgtttctg gcgctggcaa caaagctccc cccgacggta cctttcagca gattgttaac
    17761 ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag tacggtcata
    17821 ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt
    17881 taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt cggcagcagc
    17941 cagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc ttagcgtatc
    18001 gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt catccagttc
    18061 gtcctgagga atggcttcat cttcttgtgc agcttgatca atcggctcac tggcttcagg
    18121 taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg gcgtggcatc
    18181 gcggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg tgagcgaccc
    18241 caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag agcgggctat
    18301 ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat actggttggc
    18361 taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg tccccagggg
    18421 atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct
    18481 gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg ttcttatttc
    18541 atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac caagcggtgt
    18601 cagtccaaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat aacctttccc
    18661 cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggctaatt gtgcctcaaa
    18721 atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca ctaattttcc
    18781 atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat gcatgttgaa
    18841 atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc
    18901 gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg ttacctgctt
    18961 cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg ggccaaccca
    19021 aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg aataatcatg
    19081 ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag caatggcaga
    19141 agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg cctgtgttgc
    19201 acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat gtaacataat
    19261 gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca tgactactgc
    19321 aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt ttcttttggc
    19381 caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta
    19441 cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg gctggtaaca
    19501 cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt accgcagaag
    19561 ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt acgccctggt
    19621 acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca cctgattaac
    19681 gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc gcgagcattg
    19741 ttaaatcgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga tgtaaatggc
    19801 caggtggcgt tatatgacct tattgaccaa ctgcgtcgtg aactggattg tggcgtttta
    19861 atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct gtgcctgaat
    19921 caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga gtttatttca
    19981 atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca taaccatcgt
    20041 cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg attgaattat
    20101 tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt
    20161 ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc tcattacttg
    20221 gcgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtaatt gccgttacgc
    20281 tgctcctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg attgacacgt
    20341 tattagggat tatggcgcac agtgccctgt cgctgggcct ggtggtcgtt agtctgatgt
    20401 ctaacattcg tgttgatttg atggcttacc tgttcggtga tttgctggca gtgacgccag
    20461 aagatctcat cgctattgcg attggcgtgg tcatcgtggt ggctattttg ttctggcaat
    20521 ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat ggtgtgaaat
    20581 tacagcgcgt gaaattgttg ttgatgctgg tgacggcatt gacgattggt gtagcgatga
    20641 aatttgttgg cgcgttgatt attacttcac tgctgattat tcctgcagct actgcgcgtc
    20701 gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg atggtggcag
    20761 taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg tcggtggtgc
    20821 tatgtgcggc actgttattt attctcagta tgatgaaaaa gcaggccagc taatctatcg
    20881 ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt cgctatctct
    20941 ggtaggccgg ataagacgcg aatagcgtcg catcaggcac actgccaatg tcggatgcgg
    21001 tgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag tgattccacg
    21061 cccgcgtcgt cgccatacgc ccacgcggtg tacgctgcaa aaagccttgc tgaatcagat
    21121 aaggctccag cacatcctca atggtttcac gttcttcgcc aatagccgcc gccaggttat
    21181 ccagacctac aggcccacca aagaatttat cgattaccgc cagcaacaat ttgcggtcca
    21241 tataatcaaa accttcagca tcgacattca gcatatccag cgcctgagca gcgatatctg
    21301 ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc agcagacggt
    21361 tggcaatgcg cggcgtaccg cgagcgcgac gagcaacttc cagcgcgccg tcatcactca
    21421 tctcaagccc cataaagcgt gcgctgcgac tgacgatata ttgcagatcc ggcacctgat
    21481 aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc agcgaacctg
    21541 cgcgtgtggt tgcaccaatc agggtaaacg gcggcaaatc aattttaatg gagcgtgccg
    21601 ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccgga tacaacacct
    21661 cttcaacaac tggcgataga cggtggatct catcaataaa cagcacgtca tgcggttcaa
    21721 ggttagtgag catcgctgcc agatcgcccg ccttttccag caccggacca gaagtcgtgc
    21781 gtaaattaac gcccatttca ttggccacaa tgttggcaag cgtagtttta cccaaccccg
    21841 gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct gctttgatga
    21901 aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt aatttagggc
    21961 gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc agacggtctg
    22021 cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta atgtttcact
    22081 gctggcgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg gtttatagcc
    22141 cagcgccacc agcgcggcaa ccgcttcttg ttcagcatcg tcggtcgccg ggctggcagg
    22201 agacgtgagc accagatcgg cggctggcgt aaagagatcg ccgtgcaaac ctttaaatcg
    22261 gtctttcatt tcgacaatta aacgctcggc ggttttttta ccaatgcccg gcagtttcac
    22321 cagtgctccc acttcttcac gttcaacggc attaacgaac tgctgcgctg acattccgga
    22381 gaggatcgcc agcgccaact tcgggccgac gccgttggtt ttgattaact ctttaaacaa
    22441 cgtgcgctct tgcttattgt taaagccgta cagcagctgc gcgtcttcac gcaccacaaa
    22501 gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac aggtcatcgg
    22561 catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg gttgtttttc
    22621 aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga atcaaagata
    22681 atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga aaacttgcga
    22741 ggcgtcccaa cgatctgaaa aatggcgcag tataatttat tctacaggtt atattggaag
    22801 caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat acattgcgac
    22861 tcctgaaata ataaattttt tatgctatta cgtatattca tatctatttc aatggaatga
    22921 caacgtgaat attaattacc ctgctgaata tgaaattgga gatatcgtct ttacatgtat
    22981 aggtgctgcc ttatttggtc aaatatcagc tgcatcaaat tgctggagta atcacgtcgg
    23041 gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg ttcccctttc
    23101 aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct atgctataaa
    23161 gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac aggttccctc
    23221 ccggctacgc aaactttacc acaccggttt taaatacgaa tcttcgcgcc agttctgttc
    23281 aaaatttgtt tttgatattt ataaagaagc gctatgcatt ccggtgggtg aaatagaaac
    23341 gtttggagaa ctgttaaata gcaatccgaa tgcaaaactc actttctgga aattctggtt
    23401 cttaggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt ggcatcatcc
    23461 gggtttggtg ttgattcacg cggagggagt tgaaacgcct cagcctgaac tgaccgaggc
    23521 ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct catctgcatc
    23581 gcattctgac taacgtggca gtgggtgatg gcaatcgcca gtgcatcggc ggcatccgct
    23641 tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg gcttttctcg
    23701 gcgctaccaa tgcctaccac cgtttgcttt acctgacgtg ccgcgtattc aaatactggc
    23761 aactcctgat tcaccgccgc cacaatcgcc acgccgcgcg cctggcccag tttcagggct
    23821 gagtcagcgt tcttcgccat aaagacttgt tcaatggcga aataatcagg ctggaactgg
    23881 gtgatgattt ccgtcacgcc cgcatagatg agcttcagac gagacggtaa atcatccact
    23941 ttggtgcgta tgcatccgct acccaggtag gacagttgcc tgcctacctg gcgaattacg
    24001 ccatagccgg tcacgcgcga acccggatca atgccgagaa taatagccat cacgcgtctc
    24061 cgttttgctg tttagcaggc ctcatcagag agtcgctgcg acctcatcag agatttcacc
    24121 gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca gacgcatcag
    24181 tttcggtgcg gtttctgcat ccatatcagc tttggtggac gggatcatgg aaacttccgc
    24241 gctgtctgct ttcagacctg ccgcttccag agcgtcgcgc actttaccca tttcttccca
    24301 tgcagtgtag acatcaatcg cgccgtcatc ataggtcaca acgtcttcag cacctgcttc
    24361 cagagctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatca cgcctttttt
    24421 gctgaacaga taggctacgg aaccatctgt accgaggtta ccgccacatt tgctgaatgc
    24481 atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa tcatgattgc
    24541 cgtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat catcatcacc
    24601 gcccacacca cgtgcaattg cgcggttcag tgtgtcacgg gtcatgttgt tagacagtgc
    24661 tttatccacc gccgcacgca gacgcgggtt agcgtccgga tcgccaccgc ccagcttagc
    24721 cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag catcctgcgc
    24781 agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa tatctccaga
    24841 tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc cggttgctcc
    24901 aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaagcc agatgttcag
    24961 tgaaaacgat ctgccgctcg tgcggaagcg caagacagaa ccatgattcc gtattacgcg
    25021 tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaactct accgtgcgct
    25081 gacagtcaat taaggtcagt tgttcagcga caacatcaat ggtgacctct tcctttactt
    25141 cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt accgactgcc
    25201 agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct tgtgcataga
    25261 tgaccactaa gatcgaaacg ggacgcttat acactttatc ctccacgctg cctcttcgtt
    25321 gactgccttc gctcatccca ttcacatagt tatctatgct catgggagtt cactcagttg
    25381 ccgccttgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta ttctcagcct
    25441 tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag ctcggtgctt
    25501 cagtcatcag acacgctgcc gccgtggttt tcgggaaggc gataacgtca cggatattgt
    25561 cggtgccggt cagcagcatg gtcagacggt caagaccgaa tgccagacct gcgtgcggcg
    25621 gagtaccgta tttcagagcg tcgagcagga agccgaattt ctcgcgctgt tcctcttcgt
    25681 tgatacccag aataccaaac accgtctgct gcatatcacc attatggata cgtactgaac
    25741 caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc gcattttccg
    25801 gtgcagcttt cagttctgca gcagtcatgt ctttcggtga ggtgaacgga tggtgcattg
    25861 ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc cacagcggtg
    25921 cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc agtgcgccca
    25981 tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg ccatcttgcg
    26041 cggcagtacg atccaggatg gcttcgatga tttctgcatt aaggaacttc gctaccgggc
    26101 tgttgatacc ttccagacct ttcgctcgtt cgttaacttt gatgtaagcc agacctttcg
    26161 cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc agcgatgcgc
    26221 cgcccggaac gcgcagagcg gcaacgcgac ctttcggatc gttcgccgga cctgcaaata
    26281 ctgcaaactc aacagatttc agcagatcag caacgtcggt cagttccatc gggttacgca
    26341 gatccggttt atcagaacca taacggcgtt ctgcttccgc aaaggtcatt accgggaaat
    26401 cgcccagatc cacgcccttc acttccagcc acagatggcg caccagcgct tccatcactt
    26461 cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta aattcaggct
    26521 gacgatcagc gcgcaggtct tcgtcacgga agcatttaac gatctgatag tagcggtcaa
    26581 agccggacat catcagcagc tgtttgaaca actgcgggga ttgtggcagc gcgtagaatt
    26641 tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttccggc gtggctttgg
    26701 tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg cgcaccaggc
    26761 tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cgggcgacgc aggtcgaggt
    26821 agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc ggcagaacat
    26881 ctgcgcggtt gatgatagtc agcgaggacg ccagcacttc gatttcgccg gtcgccatat
    26941 cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga atgcagaact
    27001 cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg aaaaatacct
    27061 gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga tcacgacgac
    27121 ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg agctgtccac
    27181 aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc ccctgctgcg
    27241 caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa ggcggctatt
    27301 atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc tcgttaccac
    27361 tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt tgcccgaact
    27421 cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag gtgtgaggca
    27481 cgattttgtt ttttcaggag tcattatgct tgaacttaat gctaaaacca ctgcgctggt
    27541 ggtgatcgat ttacaagaag gcatcttacc ttttgccggt ggtccacata ctgccgatga
    27601 ggtagttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc agcccgtgtt
    27661 tctggtgcgc gttggctggt ctgccgatta cgccgaagca ttaaaacagc cggttgatgc
    27721 cccctccccc gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc
    27781 aactgacagt gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct
    27841 ggagttgcaa ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa
    27901 tatcggtgtt gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc
    27961 cgaagacgcc tgtagtgccg ctagcgccga gtagcacaat aacagcatca accatatcta
    28021 cccacgcatc gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc
    28081 ggtctaccgc aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag
    28141 tatgcccgcc actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa
    28201 gagcatatat tttattcata ataatcaaag cgttggattg atatatcata tgaagatcgg
    28261 taaagttcat atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt
    28321 tttgccccat gtatgcccca aaaaatcact tttcctcacc ctcaatactg tttatccata
    28381 cagttaaaaa taatgctgaa tatagacaca gtatagaggg gtttttatgc atattgaaat
    28441 ctgcatagtc acaaagaaaa tgacaaacgg tactatcgct gtaaatgaat ttgaatttct
    28501 tttgcctaaa ctcctatcat ctatatcaac aattcgtata aaaccggcat taaaacaatg
    28561 ctcctaaaac tgtaatatta cttctccaaa gctctaggac acaaataaaa tgcttttaat
    28621 atcttgactt cttcttgaaa agcattataa aagaactccg atatagaagt agcggaagct
    28681 ttatatcaag tttgattgca tgcaaaggag gacaaaatgc caacaatatc taatatccat
    28741 tcatataatc caatatactc atcgaaaacg aaagctccgc aaacatggga taccaacaac
    28801 tatctaagaa agctatcaga aattaacgat gacattattt ttaatgttca aatatctaaa
    28861 ataacacaaa aacaatacac ttttgatgaa gtagcaagaa atgaaaaaaa atacaactta
    28921 aatatttacg acattttctt ttatccccag gatgatatca tagaatacaa tataagtaga
    28981 atattacagc accaatattc gcatagtcca acactcagaa gattagttaa ctattacata
    29041 gacaacttac cagaaactga tgtaaataaa tgtcaaatac atattgcaaa taattataca
    29101 tataataaaa aagaagataa tataagcgaa ttgtttattg ccgctgatga aaaaggaaat
    29161 ctcatcgtcc cacaaaaaga aacatcagag atagaaatat ctccagagaa aattttacta
    29221 aatttcttct taaagcatat tataaaccct gaggatcttg gttatagtga tattgatata
    29281 tatactaaca ttatttttaa agaattaacc ccacaggcaa tggcgcattc ttcagaatca
    29341 tttcagcata tatctgttgg gaaggaaaac aaactattcg atagcgaaac atcagagatg
    29401 tttacagata atatcgaaca ggtaatatca aagggaaagg ctcttcagaa agagtttttt
    29461 aaaagtttta taaataataa agaaacagaa tcattcacta taaaacctga tgaatatagc
    29521 acaaaaaaaa tcgtgctcaa tggattatta ctaacgtcaa acatgtcagg aactttaagt
    29581 aataattata atcaacacaa caccaataag aatctagccg ataaaataaa catcaggcta
    29641 tcaaggtctc tccctgaaga acatgcagct ccagctggag ctgcagctcc agcgatagat
    29701 cttttcttgg cccaaaaaat agaggagcat ttaataagaa aaatatttcc tttttacccc
    29761 tcgataaaca gattagaaca agtacaagaa caaattaatt acataagagg atatcatgaa
    29821 ttacttgcat tccatcaaac aggctcatgg attcgtaatg gcagaaatgc agcaagagat
    29881 tacatagttg agctaataaa aaatcatttt tataaatttg aaataaaaaa cagtcgtgga
    29941 tattttattt tcttcaaaga ttttattcat gcacgcagag aacatgaata tagaatatat
    30001 gaagcgcttg cacgagatat aattgtgcac cctcaacctt atcacttaga ttacaaagaa
    30061 ccagcgcaag aagagctact aatatctgtt ccagaaaacc attatatgga ggcaataaga
    30121 aataagttta caaataacaa acctctaaat tttgacaata tgtacggaaa agaatctata
    30181 tcaatatcga ttgaattaga taatttaaag tcccccgggg aaaaaaacaa aaaaaacgca
    30241 gagcatttgt taaaaaacgc tcaactggtt attcataact tatcaaaaaa tagaggggat
    30301 aaaattctct gctcacccaa cggaactaaa tatcatcgtt taatattaaa catgatagta
    30361 agacaatgca agcaacataa atcaaccatt aataaaagaa taatagactc catatcaaaa
    30421 aaaataattc ataataatga tttctcagcg aatataaaaa gagaaatatc caggatgaat
    30481 agtaatcgtt acaacccatg gttaataaaa ctggctgcag aaaaacaact ttcttgggct
    30541 gaaacccact atcaagcaca acgtcacatt caagagcata tgcaggactg cgaaatatta
    30601 aatgtaatgg atgcgaataa attagtcaga gatgccatta ttagttttgt acatcagact
    30661 aatgaaataa ctgaagccag ctggatgtca tcagaagaac aacatgaaaa aaaaattcaa
    30721 gcccttaatt cttttaaaac caaaattgca tcaatggatg gggggcagga gttcatctat
    30781 agttttaaca aagttattca agaaggactg ggggggctaa tagagttaag ttttgatatt
    30841 gacgatactc gccatcatcg aaatctgtct tcactcagcc cggcatcaag aagtggactt
    30901 catttattag gttctatctg gaacatagta atgagtgctg ttcccggctt taacatccta
    30961 tcaggaagta gcagcatatt aaaccgtgct attgtcgaaa aatcaacaga tgtctgtggg
    31021 tatatacagg atgcaatacg tataggcatg gaagcattcc ctgtggctga ggctaaattc
    31081 aaaacaagag catcaaatgc caaatatact gggcttcgtt ttattgaaga gaaaattaat
    31141 aaaaacataa tcgaggctcc tttacaacga ggatcttcct ttaaagtaat agaatcaata
    31201 gaaaatactg attttattta tgaaagcagc tcaaaaaaaa tattagaatt aaatctcaaa
    31261 gaagaccatg aattatttag cgcaacaagt tttgacaata aaaaccacgg atactacaaa
    31321 cgatcaggcg atggctttta tagaaaacag cacgcgtacc agcctttatc cagagaaact
    31381 cctaacaaga taaaatataa tagtaaagaa gtagaattaa caaaagaacc gaattcagaa
    31441 atgtattctg gtacatttgt tgataatgga aaaagtacaa ttgtaaaatt ttataaaagt
    31501 tcagacggta gtttttatca agcagaagga ttaaaaggag gcggggtcat ccgacatacg
    31561 gacagaccat attcagaatt gaaagaaggt gatataggat atgatgagga gctattagac
    31621 attactgatg actcacctga gttagaagaa accttacctg cattatccca agatttatat
    31681 cctagtgaag aagaaaatgt acaaagtctt tataataaat ttaaaaatgg tgatgttgag
    31741 gcaggaatga cagaggtgac attatgtaga ggaactattg cctctcaggc tgaaaacatt
    31801 gtttcattta gtacagcagg aggagccgag attgcaaacc caaatgtaag cccagtttct
    31861 gaagatattg caaaactgca aataaaaagc gggcgaattg aaccagaata cacaactgat
    31921 attagtgttg ccgatagatt tagccgcgga catcacttag ttattgtcaa aacaaaagtg
    31981 aaatatctta caagaggcag catcagcgaa agtggctgga ttattcccaa gaacgctcct
    32041 gtcgagccag taggattaat tgacaggaca tttggtcaac cagaaaacat tcagcaagcg
    32101 aatgcatcaa aataacattt acttatatta ttcttggtat ctcagacagc ctctagcaca
    32161 gagtaatgtc aacccctctt aacatataat ttaagtgcat tagccacaga tactatctga
    32221 taccttaaat atctgtggca tttttataaa tattactgat gccaaaaatc gcctcagaga
    32281 acatatatta cactaataaa acttgagaga ataaaatacc ccatttcatt taaaacaatt
    32341 cttgagatac tttttatgcc attttcctgt attttcaatt ggttgatttt agttgaaatc
    32401 ttttttagtt ttgctgaagg gatttttgag ctgattgacg cgcaattctg aacgtttcca
    32461 atgtgctcaa aaagtccaga tctgaccgct aattactgct aatcagcatc tgcaggcagc
    32521 gctgggaact cagtatccga tactacgaat gcatcaacac ggttcagcaa cacccgatac
    32581 ttcttccagg ctgccagcaa caagttttct tcctccgttg cgatttccag atctgcagca
    32641 tcctgaagtg gcgcgatatg ctcaccggct acctgcatca ggctgttttt tgttccttcc
    32701 gcctcccgga tccgaaacag tttttctgct tctgcatctt tcacccaagc tgtgccgttc
    32761 cacttgatat tcccgatcta aagaaactgt cgcaaaagtc atggactgat ataacccaca
    32821 aaaaaatgct cctgaacgtt ttcatgcagg caaatagtaa cgattgcgtc gatgagttta
    32881 cgccagcgct ttacgctaat actcaggaga ttttgctgga gagggatatt cagcgtactc
    32941 ttgctaacac ttttcctaat gaacatccgg aggcctttat tttgattcct tcggcggaaa
    33001 aatttattgt agatggcaat tgcattcaac tgattggtat tggtaatgag cagggggagt
    33061 ttgattttta caggcaagtt tatccagtga caggtggaga gcaccaggtt gaagccttga
    33121 tgtggaccta tcccttgggc gcatatgtat cattgaatac gactgttctc cagattctga
    33181 gtaagcggct cgccggaacc gtattgatat ttactgagag ctcagatcaa ctttccaggg
    33241 caacagatcg cgtacccggt ttgccggcca gtcctggata tgctcccgcc atcattgcgc
    33301 tcccgacgac aatattaatg gccaggaaat gtttaaaaca atgattcagt ttcctaacca
    33361 tcaagtgtat atgctgactc aattagtcaa tatctatggg ttgagttcta cccaaagtgg
    33421 ctctgtagtc gcttcttctt acagtatgtt caggtgttgc aggcacagtt gtggagcgta
    33481 gatgcgttgt tggtttacca tgctctggta cataaaaaac gcctgtgcca tggtaacctg
    33541 atatagaaac gttaattccg cgacgttcaa actcggcata gacatgttct gctaaagacc
    33601 tcttttgacc aaataatgcc ctagccaacc agccattatt gatatttgcg gatttctcta
    33661 tttcatcagg agagaagtct ttgtttttta ttatgtcggc tgagttacag gatgttaatc
    33721 tgatatcatc catttcatga agattatgtt gaacaatgcg ttcgacaata tctgccggcg
    33781 ataaaaattc atctccgcac ttaagaaggg gtaggccagc agaaccatcc cctgacagat
    33841 aaattttgtt gtattttcct ggggttaaat catttggcag aatggttttc atttctgtcg
    33901 ctgcaatact tatagctgca gcggcaacaa catcgctatt atttgattgt agatggtttt
    33961 tatttgcttc aggatatgta aactccattt ttcttttatc aaaatcttgt tttgtatcat
    34021 cattcaggaa taataaatca tacggttttt ttcccgctgt gactcttgaa acattttgac
    34081 caagaatatt aagagtatat tcattaacgg atttcattcc taaaaaaaca attaatcctt
    34141 ctttctcttg agctttttcg attgttctcc ctgctaaagt tgttgggatg gattgagatt
    34201 gttttgtcgg aactgatacg ctggcgctaa taggtaggag ggatcttatg ctaaacatac
    34261 aacctctttc ttaattttcg aacctgttta gggttctgtg taaattcaaa ataaacctgt
    34321 ctgaacattc ctaacaaaca tccccctgac atggcaa
//
LOCUS       DAHBHC010000055        33629 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHBHC010000055 DAHBHC010000000
VERSION     DAHBHC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.055"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(19..94)
                     /locus_tag="M2A35_003094"
     tRNA            complement(19..94)
                     /locus_tag="M2A35_003094"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="M2A35_003095"
     tRNA            complement(153..229)
                     /locus_tag="M2A35_003095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="M2A35_003096"
     CDS             complement(332..1717)
                     /locus_tag="M2A35_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCB7199359.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="M2A35_003097"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="M2A35_003097"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCB7199360.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="M2A35_003098"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="M2A35_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCB7199361.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="M2A35_003099"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="M2A35_003099"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCB7199362.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFAARGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="M2A35_003100"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="M2A35_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCB7199363.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="M2A35_003101"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="M2A35_003101"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCB7199364.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="M2A35_003102"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="M2A35_003102"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCB7199365.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="M2A35_003103"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="M2A35_003103"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCB7199366.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="M2A35_003104"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="M2A35_003104"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCB7199367.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="M2A35_003105"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="M2A35_003105"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCB7199368.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="M2A35_003106"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="M2A35_003106"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCB7199369.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="M2A35_003107"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="M2A35_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCB7199370.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVIDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="M2A35_003108"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="M2A35_003108"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCB7199371.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTSGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="M2A35_003109"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="M2A35_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCB7199372.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="M2A35_003110"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="M2A35_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCB7199373.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="M2A35_003111"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="M2A35_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCB7199374.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="M2A35_003112"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="M2A35_003112"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCB7199375.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="M2A35_003113"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="M2A35_003113"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCB7199376.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLSPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="M2A35_003114"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="M2A35_003114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCB7199377.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22142..22591
                     /locus_tag="M2A35_003115"
     CDS             22142..22591
                     /locus_tag="M2A35_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCB7199378.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22790..23071
                     /gene="ppiC"
                     /locus_tag="M2A35_003116"
     CDS             22790..23071
                     /gene="ppiC"
                     /locus_tag="M2A35_003116"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCB7199379.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23158..24633)
                     /gene="ilvC"
                     /locus_tag="M2A35_003117"
     CDS             complement(23158..24633)
                     /gene="ilvC"
                     /locus_tag="M2A35_003117"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCB7199380.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24783..25676
                     /gene="ilvY"
                     /locus_tag="M2A35_003118"
     CDS             24783..25676
                     /gene="ilvY"
                     /locus_tag="M2A35_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCB7199381.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25728..27272)
                     /gene="ilvA"
                     /locus_tag="M2A35_003119"
     CDS             complement(25728..27272)
                     /gene="ilvA"
                     /locus_tag="M2A35_003119"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCB7199382.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27275..29125)
                     /gene="ilvD"
                     /locus_tag="M2A35_003120"
     CDS             complement(27275..29125)
                     /gene="ilvD"
                     /locus_tag="M2A35_003120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCB7199383.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29190..30119)
                     /gene="ilvE"
                     /locus_tag="M2A35_003121"
     CDS             complement(29190..30119)
                     /gene="ilvE"
                     /locus_tag="M2A35_003121"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCB7199384.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIDVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30139..30402)
                     /gene="ilvM"
                     /locus_tag="M2A35_003122"
     CDS             complement(30139..30402)
                     /gene="ilvM"
                     /locus_tag="M2A35_003122"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCB7199385.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30399..32045)
                     /locus_tag="M2A35_003123"
     CDS             complement(30399..32045)
                     /locus_tag="M2A35_003123"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCB7199386.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32048..32098)
                     /locus_tag="M2A35_003124"
     CDS             complement(32048..32098)
                     /locus_tag="M2A35_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199387.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32185..32283)
                     /gene="ilvL"
                     /locus_tag="M2A35_003125"
     CDS             complement(32185..32283)
                     /gene="ilvL"
                     /locus_tag="M2A35_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCB7199388.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32636..32809
                     /locus_tag="M2A35_003126"
                     /pseudo
     CDS             32636..32809
                     /locus_tag="M2A35_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32984..>33629
                     /locus_tag="M2A35_003127"
     CDS             32984..>33629
                     /locus_tag="M2A35_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCB7199389.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRGTPPERLKLGNYPDLSLKAAREKAALCRAWLAEGKNPRHELNYSIQE
                     ALKPVTVGDAISYWLEMHVKENRVDYVCLKSRLNNHVIQHIGNMPLDKCELRHWLACF
                     DRIRKKSPVTSGHMMQACKQALKFCRKRRYAVSNVLDDLNVVDVGKKPGISERVL"
BASE COUNT         8174 a         9192 c         8514 g         7749 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 gcgtttggtt acggtttgtg cgttttgctg attattcctc cagtttatgc gctttcccgt
      361 ggcgattaag gccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccaa
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 cgggtacgcc gtgacgggaa actttcgcca tcgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgcctattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac cttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agcgcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc cagaccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccactgcgat caatgcgggt atccactgcg ccatctccgg gaaccagaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccaa taagaccagg
     1441 cggtgagata accaaagaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaag acgaatagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcaaggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac ggagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gcacttccca ggtaaagtag ttggctgagt
     3061 tcagcaccat actcgggcga cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca tggcgagaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta gcgccagcca gaacatgccc acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgcta tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggagaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatgcc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac aacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccacc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcaccggca aatgctgttc cgtcatatcc tgccagaacg gattttcccg
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc gcacaaggca gcaatatggt cattgctgcg
     4501 gtcaccggag ttccccacca gaatggtcat tttcccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg
     4621 atgcgttttg gcaaaaaagc tcaaatcgcc gcgggcggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaagaaa aaacgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcagcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgatttccg ccagaatata aaaccgcaat gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcagccag atagcaaaat cacgcagcag ccagacggta
     5401 aaactcgccg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt tgatcaacat gatagcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ctccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacaca
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagcgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat ggaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgta tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcta ccacaaacag
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgccgct tcaatcagcg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa aacgattttt gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcaccta
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 cataaccacg aataccgccg gacgccgcac gtaaaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgtct gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctggc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccact acagcaccac tatcactgac attgttcacg ggtagtgcca
     7921 aatcaacttc accttctacc aaggagaatc ccagctgttg cagggcatcc agttcacccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccacgg cgctaacacg tctggcgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc acgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttccagcg agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccgttgagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcgtatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccaa catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc tttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcgc cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgca ccgttttacg cattccactt
     9121 tcaaaggttt cctgtggcag ccaaccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaagtcacg atagtgcacc
     9241 acgccgtgcg gcttgttcgg agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagtgc tcgctttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acagttcgct ggcgcgggtc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 acacgcgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtgagatc cggacgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtgggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actttcacgc ctgtgaccgg tcaccagaat catcttttta tcggggtcga taaacgggta
    11881 atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctgatcac gcacccataa
    11941 cagtgcatca atgactgtat taccagtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gtaaaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 tgttgtgtcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagcacctga tcgagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct cgcagcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggcccgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gggtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgct
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tctatgaccg atggttggtc ggcagaagcc atgtttgaac
    13321 gggcatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgcgctcc actcttgtcg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg gacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac tttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtctta gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcggttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catacgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcacccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccacg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccatgccg
    17281 acgcagaacc gctggcggaa gctaccggcc tgaagctggg tctggcttac ggtggtgatg
    17341 gctacgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcactacgg
    17401 ggcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtggtgg
    17461 tactggacga agccgatcgc atgtacgatc tgggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac
    18181 gtacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcatact gtagtaagtg attcaggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaata
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt aagtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacgct gcgtaatatt
    19321 cagcgccgct ttatgatcga tattgatcag gcgcagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa ggaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact ttaaacaagc gccgcaacac
    19501 gctgcttatc tggtgcgtaa tctggatctt cccggtttta cccccgcaca gaaaaaattg
    19561 ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggccatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaagccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tttccgccct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagctcgc cgtactgacg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtgcagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtaaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctctcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacactcg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaacttta agctctgtgc
    21001 cgtaacccag ttcggagaac agacgcttct caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctga ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ctcatactgg ctggtgtttg tgtcctgata ctcatccacc agcagatagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagcagc aacgtcggca
    21361 ataaaatcag atcatcgaag tcgagaacat tgcacgcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cgatcccgct cgccctttgc ctctgccgcc gcctgcgccg
    21481 gagttttgag atcgttcttc cagttggaga tggtcgaaat cagctgttgc aggagaactt
    21541 tgtcatcttc aatcagcccc tcggtcaact ctttaagcaa agcaagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccaa gcgccgcata ctcgcgtttg atgatatcca
    21661 accccagcgt gtgaaaagtg gagatcatca gcccgcgcgc ctctttgcga cccagcgtct
    21721 gcccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg atcacacgag
    21841 ttttaccgga acccgcgccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggtg tattgctcaa tcttcgaacg gggggaggat
    21961 tgtagcagaa tcgcatttga atgtgaccgc gtgtaagccg ctaaccggtt ggctgcggat
    22021 gtttaaatca gagagagaag accgaaatcg gagttcatcg tgaatccatt agacttagaa
    22081 aatatcgggt cgtaacgttg tgttattact ttttcagagt ttactaagga gaggtttact
    22141 cgtggcgatt aatttcagcc ctaaggttgg tgaaatacag gaatgcaact ttggtaacta
    22201 tcctgttatc cagaatggac agttcagcac cacgtattac gatggccgca ttcctcctga
    22261 aatgataaaa aacaggttag ttgtcgtgct caacggaaaa attaatggca acgcatgtat
    22321 cgtcgttcca ttatcaacca ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga
    22381 aatagccagt aatgttatca atgtcctgca attctttgat cagcagattc gttgggccaa
    22441 agccgacctg gtacaacaag tcagtcgaaa ccgattaaat agagcaagga cttacagagg
    22501 gtatcttaat caatgtttgc cacatgagtt ggttgcagac atacagaggg cggtcatcaa
    22561 gtcaatcaat gccatatcgc tcataaattg acaataaaat aaccatcccc gataatgact
    22621 ttgtagccca tagaggccct aagttttctc cccttgaggg acctgcgaag cccggatttt
    22681 ccgggctttt gccatatatg taccagcccc tccctgactg acaattacgt tagcatctgc
    22741 ggatcatgct aaaatcgccg cctgacaatt attcactcaa ggcacgatca tggcaaaaac
    22801 agcagcagca ctgcatatcc ttgtaaaaga agagaaactg gctctggatc ttctcgagca
    22861 gattaagaac ggtgccgatt tcggcaagct ggcgaagaaa cactccattt gcccatcagg
    22921 caaacgcggc ggtgatttag gtgaattccg ccagggtcag atggttccgg cgttcgataa
    22981 agttgtgttc tcttgtccgg tactggagcc gaccggcccg ctgcacaccc agttcggtta
    23041 ccacatcatt aaggtgctgt accgcaacta atagcaaggc cttctccagg agaaggcctt
    23101 gagtgttttc tccctctccc tgtgggagag ggtcggggtg agggcatcag cgcgcactta
    23161 acccgcaaca gcaatacgtt tcatatctgt catatagccg cgcagtttct tacctacctg
    23221 ctcaatcgca tggctgcgaa tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc
    23281 gccttccgga atagctttac ccaagtcgcc cggttgcagc tctgccataa acggtttcag
    23341 caacggcaca caagcgtaag agaacagata gttaccgtac tcagcggtat cagagataac
    23401 cacgttcatt tcgtacagac gcttacgggc gatggtgttg gcaatcagcg gcagctcgtg
    23461 cagtgattca taatatgcag actcttcaat gatgccggaa tcgaccatgg tttcgaacgc
    23521 cagttcaacg cccgctttca ccatcgcgat catcagtacg cctttatcga agtactcctg
    23581 ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag gcggttttgc cggtctcttc
    23641 acgccaggtc agcagtttct tatcatcgtt ggcccagtcc gccatcatac cggaggagaa
    23701 ctcgccggag atgatgtcgt ccatatgttt ctggaacagg ggtgccatga tctctttcag
    23761 ctgttcagac agcgcgtaag cacgcagttt cgccgggtta gagagacggt ccatcatcag
    23821 ggtgatgccg ccctgcttca gcgcttcggt gatggtttcc caaccgaact gaattagttt
    23881 ttctgcgtat gccggatcgg tgccctcttc cacgagcttg tcgaagcaca gcagcgagcc
    23941 agcctgcaac ataccgcaca ggatggtttg ctcacccatc aggtcagatt tcacttccgc
    24001 aacgaaggac gattccagca cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt
    24061 agcgatcgcc ataccttcgc ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg
    24121 tacgccgaat ccacgtttgt actcttcacg cacttcggtg ccagggcatt tcggcgcaac
    24181 catgactacg gtgatatctt tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg
    24241 cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt acggtgcgca ctacatcaga
    24301 gtgctgcttg tccggcgtca ggttaaccac cagatccgcc tgcgggatca gttcttcgta
    24361 agtaccaact ttaaaaccgt tttcggtcgc tttacgccag gacgcgcgct tctcggcaat
    24421 cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt tcaggccctg
    24481 gttcagcccc tgtgcgccac agccgacgat aaccactttt ttaccctgaa ggtagctcgc
    24541 gccgtcggcg aattcgtcgc gccccataaa gcgacatttg cccagctgtg ccagctgctg
    24601 gcgcaggttc agtgtattga agtagttagc catggtgatt cctcgtgatg ttgtgcttct
    24661 tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat ttattgcgga
    24721 aattgatata ttcacaacgt cacattgcaa tttctgcaac gtcaacattg agggctgcgc
    24781 cattggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc cattttggcc
    24841 gcagcgcgcg ggcgatgcac gttagcccat ccacgctctc acggcagatt cagcgcctgg
    24901 aagaagatct tggtcagccg ctatttgtgc gcgataaccg cacggtaacg ctgaccgaag
    24961 cgggcgaaga gttacgcgtt ttcgcccagc aaacattgtt gcagtatcag caactgcgcc
    25021 acaccatcga tcagcaaggg ccgtcgctct ctggcgagtt acatattttc tgctcggtga
    25081 ccgccgctta cagccatcta ccgccgattc tcgaccgctt ccgcgcggaa cacccgtcgg
    25141 tggagatcaa actcaccact ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag
    25201 aagcggatct ggcgattgcc gggaaaccgg agacgctacc cggcgctgtg gcgttttcga
    25261 tgctggagaa tctggcagta gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc
    25321 aggtatctgt cgaaaagccc gactggtcaa cggtgccgtt tattatggcc gatcaggggc
    25381 cggtacgccg ccgcattgaa ctgtggtttc gacgcaataa aatcagcaac ccgatgattt
    25441 acgccacggt tggcgggcat gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg
    25501 cgctgttgcc ggaagtggtg ctggaaaaca gcccggagcc tgtacgcaac cgggtaatga
    25561 ttttagatcg cagcgatgag aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc
    25621 ggctgcatga gccgctgatt gaggcgttct ggaggatttt gccgaaccac aaatgacgtt
    25681 gtcgcgcggg taggcctgat aagcgaagcg ctatcaggca tttttcccta acccgccaaa
    25741 aagaacctga acgccggatt attggtttcg tcgtggcaat cgtagcccag ctcatttagc
    25801 cgggtttcga aatccggttc atggtcgccg agttcgaacg ccgccagtac gcgcccgtag
    25861 tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg
    25921 aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag gcgttcctgc
    25981 aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc catttcgtcg
    26041 tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc
    26101 tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt ggcatcggca
    26161 aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa tttgaggaag
    26221 ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg
    26281 cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga aagaatatgc
    26341 gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc
    26401 agcgcgccag agggttccgc caccgcgcgc acatcttcga ataaatcctt catcgccgca
    26461 cagatcgcat cactatcgac ggtgatgatg tcgtcgagat actcctggca taaacggaag
    26521 gtttcgtcac cgatgcgttt taccgctacg ccttcagcaa atagccctac gcgcggcaga
    26581 tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc cgcttctacg
    26641 gcgatcactt tgatttgtgg catcagttgt ttgatcagca ccgcaacgcc cgccgccaga
    26701 ccgccgccgc cgactggcac aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc
    26761 agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg cacccaggtg
    26821 aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca
    26881 tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc ggcggtggcg
    26941 gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc
    27001 gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc
    27061 gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg atcttcgcgc
    27121 ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg tagcggcgta
    27181 acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc
    27241 ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc gatttatcgc
    27301 gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa
    27361 cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac
    27421 gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc gggatgtcga
    27481 tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc gcttccggtg
    27541 agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg
    27601 cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc atttcctgca
    27661 tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc gcgacaactt
    27721 tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc gccgggccgg
    27781 tgaatttgag gatgctgtca tcgacgcccg cggttttaac gatgcagccg ttttctgcga
    27841 aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga
    27901 tacagccatt ggcgcgatcg tcatcgagag aatcccaacg gcaatcctgc gagaatgcct
    27961 gtgtggtacg aatgcctgca ggacctgcgc ggaacatatt ttttaccgcg tcatcctggg
    28021 tcagcataac gtcgtattgt tccagcgttt gcggcaacgt caggccaagt acgtttttca
    28081 catcacggtt cagtaacccc gcgcgatcca gttcgccgag aataccgata acaccaccag
    28141 cacggtgaac atcttccata tggtatttct gggtgctcgg cgcaacttta cacagctgtg
    28201 gaaccttgcg ggaaagctta tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg
    28261 ccgccgccag caggtgaagt acggtgttag tcgatccacc catcgcgata tccagcgtca
    28321 tggcgttttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt tcgtcgtttt
    28381 gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg aacaactgct
    28441 tacggtcggc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacaga cccagcgctt
    28501 cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg caggtcggac
    28561 acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct gcgccctgga
    28621 tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt ttcccggcct
    28681 ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa gccatcagca
    28741 tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt
    28801 tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc
    28861 ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct ttggcaacgc
    28921 cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga tggacgtgac
    28981 ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttaccg aaatcggcgt
    29041 cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga ccatgagtgg
    29101 tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc tcgtaaatgg
    29161 gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa ccagccccat
    29221 ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg tttggtaacc
    29281 gggccacaac ggccttcgcc aacctgaata ccgtctacgc tgcgcactgg cgtgatttct
    29341 gccgccgtac cggacataaa cacttcatcc gccaggtaca gggattcgcg cgacagaacc
    29401 tgctcacgca catcaattcc cagctctttc gccagtttga tgatggcatc acgggtaata
    29461 cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt cacttcaaac
    29521 aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat accttcctga
    29581 taaccgtggc ggcgcgcttc gctacccacc agcagggaag agaggtagtt accaccggct
    29641 tttgccgccg tcgggatggt gtttggtgct gcgcggttcc aggaggaaac catcgcatcg
    29701 atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc agcgataatc
    29761 acgtcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc gacgaagatc
    29821 agcggacgga tataggcgct ggtgagatta tttttgcgga tcacgtcacg acaagcttcc
    29881 atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga gtcatgcaga
    29941 cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc gtagcaacgg
    30001 atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg caccttcgcg
    30061 tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt cgtggtcatt
    30121 tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc tctggcagat
    30181 ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt cgaccgaccg
    30241 tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg cagccatatt
    30301 cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc
    30361 cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt ctccaacatt
    30421 tctgaattac tggcgccagg cggcaccagc ggccagacgt tctcaagttc gtcgattgag
    30481 acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc ttcaacctgg
    30541 tctttacggg tgatgtgttg gccagggatg ccgaaggcgc tggctaacat gaggaaatcg
    30601 gggttatcag taagggtggt ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt
    30661 cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt
    30721 tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga gatacagacg
    30781 acagtatcgt tcggtcgtgc gacttgtgcg ccaaccgccg ctggtaaacc gaaacccatg
    30841 gtgcctaagc cgctggaggt aatgaaattt tccgggcgag tgtgtgcgat gtgctgcgcg
    30901 gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg tttacgatcc
    30961 gacagttgtt ttaacaacaa tggcgcgtag atagcgtcac cgggatggtc gtaacgccag
    31021 gcatgttcat cacgcagctg cgcgcagtgt agctgccagt cattgatatt taacggctgc
    31081 tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc
    31141 ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg tgcgaaggtg
    31201 ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac
    31261 tcctgcaccg cgaagttcgc cgctttggtg ccatgcattc ccagcatgcc cagatagtac
    31321 ggataatctg cttcaactgc gcccagccct ttcagcgtgc aggtggcagg catttttgtg
    31381 gtagcgagaa attctcgtaa agcaggaact gcctgcgcca tacccacgcc accaccaacg
    31441 tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcaac ttcggcatgt
    31501 gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccaggtcacc gctggctagc
    31561 tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct ggcaacgtcg
    31621 aatgcttcag ccataatgcg cggcaactct tccagcgact gcaccagaaa gctgtgcttg
    31681 gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtccgt gccgataaac
    31741 ggtgcggaca cttgaccggt gatggcaaca acagggatag aatctaacag tgcgtccgca
    31801 agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac gccagttttg
    31861 ccggtagcac gggcataacc gatagccgcc attgccgcac cctgctcatg tcggcacagc
    31921 aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc gccacccgga
    31981 taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca
    32041 ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg ttattcattg
    32101 tctgctcctc ggttatgttt ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg
    32161 tcttagttcg ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg
    32221 tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta gaagggctgt
    32281 cattttgtct tttcttgcat cttgttcgaa ggaatgccta aagagttacc atagattttg
    32341 cgaatagcac aagatatttt ttggccgatt tttatgatgc gtttaataat ttcaatagaa
    32401 attgttgttt tttaaaaaga aaatagtgag tgaaaaaaat tcaatttcag tatgaatgtt
    32461 gttcccttcc tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc attccaccgc
    32521 cagaacaact caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat
    32581 tagcgggcta cagcagcgat atcttatgca cataatagtg actcaaggag ggcttatgtc
    32641 actgtcaatt gttcataccc gcgcagccct gggagtaaat gcgcccccaa tcactgttga
    32701 ggtacatatc agtaaaggtc tacccggctt aacgatggtg ggcttaccag aaacaactgt
    32761 aaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat agcggatttt tataaattag
    32821 tgttttttgc tgtttcataa tgtgccacgt tgttcattat gtttttgatt tttaagtcta
    32881 ttgcaatttt cattgttcca gcttgttcca cgttgtaccc ctttgtgcaa tgatcggtgt
    32941 agtcactggt gtagtcattt tttttgcaaa caggggagga aatgtgagca gagcaatgaa
    33001 caaactgagc gacacattgt taagaaaact tcatggtaag ccggcggaga aaaatacgtt
    33061 ttatagtgat ggcggaaatc tgagcgtgaa atatttaaca tcagggaaat tgacctggta
    33121 tttcacttac agggcaggaa cgggaagagg tacgccaccg gaacgcctga agctgggaaa
    33181 ttatccggat ctgagcctga aagcagccag ggaaaaagca gcactgtgtc gcgcatggct
    33241 ggcagaggga aaaaatccac gccatgagct taattactcc atacaggaag cactaaagcc
    33301 ggtaacggta ggcgatgcga tcagctactg gctggaaatg cacgtaaagg aaaaccgtgt
    33361 cgattacgta tgcctgaaaa gccgcctgaa taatcacgtc atacagcata ttggcaatat
    33421 gccactggat aaatgcgagt tgcgtcactg gctggcctgt tttgaccgga tacgcaaaaa
    33481 atcccccgta acgtctggtc acatgatgca ggcgtgtaaa caggcgctta agttctgccg
    33541 taaacgtcgc tacgcagtca gtaacgttct ggatgatctc aacgtggtgg acgttggcaa
    33601 aaagccgggc ataagcgaac gcgtgctta
//
LOCUS       DAHBHC010000056        32715 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHBHC010000056 DAHBHC010000000
VERSION     DAHBHC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.056"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..242)
                     /locus_tag="M2A35_003128"
     CDS             complement(<1..242)
                     /locus_tag="M2A35_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCB7199390.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="M2A35_003129"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="M2A35_003129"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCB7199391.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="M2A35_003130"
     CDS             2255..3904
                     /locus_tag="M2A35_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCB7199392.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3955..4557)
                     /locus_tag="M2A35_003131"
     CDS             complement(3955..4557)
                     /locus_tag="M2A35_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCB7199393.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5077..5976
                     /locus_tag="M2A35_003132"
     CDS             5077..5976
                     /locus_tag="M2A35_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199394.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="M2A35_003133"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="M2A35_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCB7199395.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="M2A35_003134"
     CDS             7449..8771
                     /locus_tag="M2A35_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCB7199396.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9005..11065)
                     /locus_tag="M2A35_003135"
     CDS             complement(9005..11065)
                     /locus_tag="M2A35_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCB7199397.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="M2A35_003136"
     CDS             complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="M2A35_003136"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCB7199398.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12134..13063
                     /gene="kdgK"
                     /locus_tag="M2A35_003137"
     CDS             12134..13063
                     /gene="kdgK"
                     /locus_tag="M2A35_003137"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCB7199399.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSRKKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13159..14655)
                     /locus_tag="M2A35_003138"
     CDS             complement(13159..14655)
                     /locus_tag="M2A35_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCB7199400.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="M2A35_003139"
     CDS             complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="M2A35_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCB7199401.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="M2A35_003140"
     CDS             complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="M2A35_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCB7199402.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLDQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="M2A35_003141"
     CDS             complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="M2A35_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCB7199403.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="M2A35_003142"
     CDS             complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="M2A35_003142"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCB7199404.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="M2A35_003143"
     CDS             complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="M2A35_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCB7199405.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMLGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="M2A35_003144"
     CDS             complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="M2A35_003144"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCB7199406.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLVGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27948..28709)
                     /gene="bcsQ"
                     /locus_tag="M2A35_003145"
     CDS             complement(27948..28709)
                     /gene="bcsQ"
                     /locus_tag="M2A35_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCB7199407.1"
                     /translation="MAVLGLQGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPD
                     NLLRLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQ
                     TRLSDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRL
                     HQQALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPV
                     GEYRSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28721..28909)
                     /locus_tag="M2A35_003146"
     CDS             complement(28721..28909)
                     /locus_tag="M2A35_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCB7199408.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29182..30696
                     /gene="bcsE"
                     /locus_tag="M2A35_003147"
     CDS             29182..30696
                     /gene="bcsE"
                     /locus_tag="M2A35_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCB7199409.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRH"
     gene            30750..30941
                     /gene="bcsF"
                     /locus_tag="M2A35_003148"
     CDS             30750..30941
                     /gene="bcsF"
                     /locus_tag="M2A35_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCB7199410.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30938..32617
                     /gene="bcsG"
                     /locus_tag="M2A35_003149"
     CDS             30938..32617
                     /gene="bcsG"
                     /locus_tag="M2A35_003149"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCB7199411.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
BASE COUNT         7733 a         8941 c         8228 g         7813 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 aacaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcatggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc tgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgaa taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5401 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5461 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagcaccta gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gcgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt atcgatgatc ccgattttga tggtgtcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcgc tttattctgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6601 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttt agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggcagatgcc tttggtcgcc
     8401 gtaaaagtat ggtaattatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cgctgcttgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatggggct gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccaacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8941 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     9001 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     9061 gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa
     9121 cagcaccacg ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc
     9181 cacgcacata cagcggcgaa cgcagtgtga taatccgaat ccctttactc tccggatcaa
     9241 tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta atcaacgcgt
     9301 tctcagtatc gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata ttcagattcg
     9361 ccgccgcgca gttcacccgc acctcatcgt caccaaatat cgcaccgaca atgtagttgc
     9421 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9481 gtttcaggtt gccgttactg ttgcctaaaa gtgctgccac cgagttaccg ctaccgcgta
     9541 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9601 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9661 cgccttccag atgaatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt
     9721 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9781 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9841 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9901 ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggagtca acgccaatta
     9961 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
    10021 gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcgcca attcgcccat
    10081 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
    10141 catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
    10201 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
    10261 tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcagcc tgtaccggaa
    10321 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg
    10381 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10441 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaacggat
    10501 caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatagcgatc cgcccttgat
    10561 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10621 tatcatcgtt ggcgagatta aacgtccagt tattgttctt ttcggagagg cgaatcaggc
    10681 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10741 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10801 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccacggcacc cagctgcgcc
    10861 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10921 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10981 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    11041 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    11101 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    11161 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    11221 tctgaacatc acgccactct tccggtgttt ctacgccctc gacaatcacc ccgcgacaat
    11281 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg gccttccggc gactgacgca
    11341 gcatcacgaa cagctctcgc gcgattttga tgtagtcata acgcacttca cttagcgcag
    11401 agaaatttgc catcccggtg ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11461 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11521 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11581 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11641 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11701 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11761 ccacggccat taaccgtccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11821 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11881 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11941 tcgacaaaga ttatcggcgc gcaagggcag aactttaccg cctggccggg gtaaatttcc
    12001 tcactccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    12061 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    12121 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    12181 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta
    12241 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    12301 ggacagtttt agccagcaaa tgctggacgc ctggcacggc gagaacgttg atacttccct
    12361 gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg
    12421 cgagcgtacg ttctactact ggcggaacga agccgccgcc aaattctggc tggagagtga
    12481 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12541 tagcctggcg atcttaagcc cgaccagccg caaaaagctg ctttccctgc tgcgcgaatg
    12601 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12661 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12721 gacgctggac gatgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12781 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12841 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12901 gatcgatacc accgcagctg gcgactcttt cagcgccggt tatctggcgg tacgtttgac
    12961 gggcggcagt gcggaaaacg cggcgaaacg cgggcacctg accgcaagta ccgttattca
    13021 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    13081 ggctggataa agtgttactc ctctatccag ctcatccata aatacctgat ggttgtgcta
    13141 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    13201 atccgtggcg acagaggtgg cggcagcagc agtcgatggg gccatgattt gatcccaggc
    13261 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    13321 catatcattc gacaactgct gacgcagatc ctgatttaac atctccaccg tcaggctgtt
    13381 aaggaaatcc tgccgcagtt tctgatactg ctccggcgcg atatcgacga cctgattttg
    13441 caacgaacgc atccgctgac ccatcagaat atcggtatca gcccgcgcat aggcggcaaa
    13501 cagtttctgc agctccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13561 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13621 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13681 aaatccaaga ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa
    13741 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13801 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13861 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13921 tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc gcgcatccac
    13981 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    14041 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    14101 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgccac
    14161 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    14221 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    14281 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    14341 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    14401 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactttcgg cgagcgaacc
    14461 ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14521 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14581 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagtcgaat
    14641 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14701 gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14761 tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc
    14821 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag
    14881 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14941 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    15001 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    15061 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    15121 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    15181 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    15241 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    15301 atttgcggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    15361 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    15421 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15481 tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15541 tggtaaacgc cattgccccg aacgcaccaa taggcgccag acgcatgatc atattgatga
    15601 tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc
    15661 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15721 cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15781 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15841 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15901 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15961 tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    16021 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    16081 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    16141 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    16201 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    16261 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    16321 agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc
    16381 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    16441 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16501 cgccagcatg ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat
    16561 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16621 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16681 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16741 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16801 cgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16861 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16921 gagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16981 ttgctggata cgcagtaaca cttccgcact gaccagttta ccgctggtca tctcgacctg
    17041 tggctgtaac caaattgcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    17101 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    17161 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    17221 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    17281 aatcggcagg cgctcgctca tgatagtgag cacttgctga tctaaggtga ttgcgtgcca
    17341 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    17401 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    17461 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17521 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17581 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17641 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17701 cagacgcggt aatgccagtt ggtgaccaac aagctcctgg gctgggatgg cgttaagttc
    17761 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17821 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17881 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17941 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    18001 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    18061 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    18121 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    18181 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgttgga ccagatggaa
    18241 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    18301 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    18361 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    18421 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18481 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18541 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18601 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18661 gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc gtgaatgcga
    18721 ccacgagcca gacgccccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18781 cggtatggca aacgacaagt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18841 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18901 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18961 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    19021 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    19081 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    19141 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    19201 gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc agctccagac
    19261 gtcatttcgc cagccgaccg ccacgctggc accggaatcc gactggctgc ggttgccgct
    19321 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    19381 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    19441 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19501 gctcgaaccc cagtaatcgt gctcaagggt gacgttaaga tcctgctggc gatagaggtc
    19561 cgccgcatcg ctgcgcacgc cgcgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19621 tcgggtgaat gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19681 gtctttgtag gtttccagcg cctgctttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19741 gtcgcgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19801 cttattaaac gtctgctgcg ccgctgcggt atctccaagc tgcgcctgcg ccagcgccac
    19861 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19921 tgccgccgct gtgtcaccgg cagcaatatc cacttccgtc agccccagaa tggcatcggc
    19981 gttagttggc tcccgcgtca ggacattctg atatgcagcg cgggcggcgg tgtaatcacg
    20041 tcgctgctgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctgacg
    20101 cagcatcgct tccgcttcag cttctttgcc gctttctcgc aggcgattag cagtttccag
    20161 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcgcg
    20221 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agaggtacag
    20281 cccgtaagcg taaacctgct ccgggtcgtt cggcttctgc tgcgccagat tgcgcattaa
    20341 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    20401 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    20461 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20521 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20581 tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt tagtgttgcc
    20641 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20701 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20761 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20821 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20881 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20941 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    21001 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    21061 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    21121 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    21181 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    21241 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    21301 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    21361 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    21421 caccgtactc cagtactcga cagcaatgtc accttccggc ggcgcaccgt tgaacagttt
    21481 gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc gtgcctgttg
    21541 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21601 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21661 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21721 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21781 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21841 agccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21901 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21961 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    22021 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    22081 ccgaccggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    22141 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttttgc
    22201 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc gtagctgctg
    22261 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg cacccagtct
    22321 ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc gcgcagcgtg
    22381 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    22441 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22501 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22561 tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22621 acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22681 ccccacagcc aggcgggcaa acgttctttt aaagagccct gagcgagatt gttctgcgtc
    22741 cagtcgagaa tattatcgaa agctacacgg tcgttagccg ccagggcaaa gaacataccg
    22801 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22861 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22921 acactaaagg cagccaacag cagcatcgtc acgattccac tacgcaacac attcatcttc
    22981 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    23041 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    23101 gccagcgcat accacaagcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    23161 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    23221 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    23281 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    23341 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    23401 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc
    23461 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23521 atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23581 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23641 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23701 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23761 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23821 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23881 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23941 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcaggttg
    24001 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    24061 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    24121 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    24181 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tacccagttc
    24241 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    24301 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag gtctttgtcg
    24361 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    24421 atcacagggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24481 gcaaagacaa tggcattgcg atccggcagt tggttataga gtaccgggaa gttctgccca
    24541 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24601 acatccggcg cacccgcaaa gaccatcggc aaggtgttgg tgcggttatc acgcggatca
    24661 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24721 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24781 acgtcctgat aatggccgac aaactccagc cgcacacggt tgaagtcggt aataaacagt
    24841 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24901 acgcccatca gctcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24961 ggcgatggcg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcata
    25021 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggc
    25081 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    25141 gcatcagcgc cctgcacgcc cagcatcact tgccccacct gcggattttg ttccgtctgg
    25201 gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg
    25261 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    25321 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    25381 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    25441 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25501 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25561 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25621 gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct gggtcgggaa
    25681 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25741 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25801 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25861 tcggcgtacc tgtttgcttt ctaccgatac cgcaactgcg ccgccaagaa caatcaggtt
    25921 gtagaacacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25981 gtagcgccag atgcctaccg caacgcccac caggttgagt agaacaagga agatgtaggg
    26041 ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    26101 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    26161 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttcga
    26221 gttggtcagg ctggcatgga tcatatgcgg cagcacgaac agggcgatca tcaacgctgg
    26281 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    26341 tcgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgggcgaa
    26401 tttcagccct ttaccggtaa gcgggttatc gagacggaag atttgcacca tcccgcgtgc
    26461 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26521 cggaatacgc atatatgcag aggtgtagcc acgacggtgc aggcgcagag acgtgtgcgc
    26581 atcttcggtt accgtttcga cagcaatacc gccaatttca tccaatggtt tgcgacgaat
    26641 tactgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26701 accatagaac agcgtgcctt cgttcggtgt tttacggaaa cgccccaggt tgcgttcaaa
    26761 cgggtccggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt tctctttcag
    26821 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26881 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26941 ttcatgagtg gtgcgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    27001 ttccctgccg ccgtcatcga ggatccagat gttcagtttg tctttcggcc agtcgatacc
    27061 cagcgaggcg taaatggtat ttttcaccac attgagatct tcgttgtaag tcgggacaaa
    27121 gatatccacc gacggccaca gcgacatatc cttcggcaat ggcaccggct gacgattcag
    27181 cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg tttcagcgaa
    27241 gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg tagaggtgta
    27301 acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca ttagcgccga
    27361 gaagcgcccc ggcatccgcc gtacgatcag cgctacaccc cacagcagca tcaggaagat
    27421 aaactgcgcc agcgggttaa acggttgagt aacgcagatt aacgccagaa tcagcgagaa
    27481 ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac cgagctcttt
    27541 tttctcatca agatgctgtg ttttatggct aacgcgctca ggcagctcgt tcatccattg
    27601 atggtaacgt ccacgaatat tctgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt
    27661 ttctttgcgc gatgtaccga ccagtaacca gcatgtttga atgagataac ggaccgggtc
    27721 cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt ctgcgcgaat
    27781 acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca tccagaaaca
    27841 gccgagcgtc gcgctgaaag ccgacgcacc gtgacgacga taatcgcgat aacgcccgat
    27901 aagccgcgcg ttgaccggcg ggataagcaa ccaccgggtc aggatactca tgatgcactc
    27961 ccgactggcg ttttcagccc ggagtagttc aacaggcacc agtttgccag cgtcagtatc
    28021 tcttcagccg ccagcgcatc actgcgatat tcacctactg gttgcttagc cgccaggcat
    28081 tcagccatcg cttcatcacg atgaatgagc atcggcagta atcggcgctg gctttgcaac
    28141 caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagttatt aatcaaaata
    28201 tgtgcgccat ccggcagcgc ttgctgatgc agtcggatat ggcagttggc atccacattg
    28261 acgattgcca gcgagtgatc gcacaaactc agcagctggt gggttatctg cgaggcatca
    28321 cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg ctgtaagccg
    28381 gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc ttcaatggat
    28441 aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccagc gtcacgccag
    28501 tcctggccat ccagcatcgc tctggcccag ccctgacggt gggtaaaatc aacattaaat
    28561 gacaggcgca acaagttgtc cgggcaggca tcgaccacca gaacattttc tcccaacatt
    28621 tgtaatgacc aggctaatgc ggcggtgatg gttgttgtcc ccacgcctcc ccgcaccccc
    28681 tgcaatccct gcaatcccag tacggccatt caccaatccc ctacttttgt tgcgcaaact
    28741 ctgccagcaa cggccagcgt tttaatgccg cggccaactg ttcgcgttgg gaaatatcgg
    28801 cataatcaat atcaggcagt gaaaatgcct gctttaacgc cacaatatca ttctggaaga
    28861 tatagcctat cgcgggatca ggcagagtat ctggttcatt gttattcatt ttgcttgatc
    28921 cctatgaaat aaccgtaccg ttcatgagac aattctaacc gtgagcattt cgccggttta
    28981 ttgtttacgg cgagacggga atttgctcac tttttgactt tccgttacgc aacagaaaaa
    29041 gttaaaaacg aaaagactaa aatcacacgt cttgtgacct tctaaattga ttcacatgct
    29101 aaatctgata agttttaatt tcaatggtag gtttatttct tagctttcgc tagtaaactg
    29161 ataatcagac aaaatggtga catgagggac attgtggacc ctgtattctc tatcggtatc
    29221 tcatcattat gggatgagct gcgacatatg ccagcaggcg gcgtctggtg gtttaacgtc
    29281 gatcgccatg aagatgctat cagtctggcg aatcaaacaa ttgcatccca ggctgaaacc
    29341 gcacacgtcg cggtcattag catggacagc gatccggcga aaatctttca attagatgat
    29401 tctcaagggc cggaaaaaat aaaattattt tcaatgctaa atcatgaaaa aggtctatac
    29461 tatttgaccc gtgatttgca gtgttctatt gatccccata attacctttt tattcttgtt
    29521 tgcgcaaata acgcatggca aaacattcct gccgagcggc ttcgctcatg gttggataaa
    29581 atgaataaat ggagcaggtt aaaccattgt tcgcttttgg taattaatcc cggaaataat
    29641 aacgataaac aattttcatt gttgcttgag gaataccgtt cactttttgg tcttgccagt
    29701 ttgcgttttc agggtgacca acatttgctg gatattgcct tctggtgcaa cgaaaaaggg
    29761 gtcagcgccc gtcagcagct tagcgttcag caacaaaatg gtatctggac attagttcaa
    29821 agcgaagagg cggagatcca accacgcagc gacgaaaaac gcattctgag taatgttgct
    29881 gtactggaag gtgcgccgcc gctatcggaa cactggcaac tgttcaacaa taacgaagtc
    29941 ctgttcaatg aagcccgtac cgctcaggcg gcgacggtgg tcttttcttt acagcaaaat
    30001 gcgcaaatcg agccactggc ccgcagcatt cataccctgc gtcgccagcg cggtagtgcg
    30061 atgaaaatcc tcgtgcggga aaataccgct agcctgcgcg ccaccgatga acgtttgtta
    30121 ttggcctgcg gtgcaaatat ggttattccg tggaatgcgc cactctcccg ttgtctgacg
    30181 atgatcgaaa gcgtgcaagg gcagaagttt agtcgctatg tgccggaaga tatcactacc
    30241 ttgctgtcaa tgacccagcc gctcaaactg cgtggtttcc agaagtggga tgtgttctgt
    30301 aatgccgtca acaacatgat gaataaccct ctattacctg cccacggtaa aggcgttctg
    30361 gttgccctac gtccggtacc gggtatccgc gttgaacaag ccctgacgct gtgtcgccct
    30421 aaccgtaccg gcgatatcat gaccattggc ggtaatcggc tggtgctgtt tctctcattc
    30481 tgtcggatta acgatctgga taccgcgttg aatcatattt tcccattgcc tactggcgac
    30541 attttctcaa accgtatggt ctggtttgaa gatgatcaaa tcagtgccga gctggtgcag
    30601 atgcgcttgc ttgccccaga acaatggggc atgccgctgc ctttaacgca aagttctaaa
    30661 ccggtcatca atgccgagca cgatggtcgc cactagcgac gaataccaga acccatgcga
    30721 ctgttagatg atgctgtgga gcgctcatca tgatgaccat cagcgatatc attgaaatta
    30781 ttgtcgtttg cgcactgata tttttcccgc tgggctatct ggcgcggcac tctttgcgac
    30841 gcattcgcga caccttacgt ttgttctttg ctaaacctcg ttatgttaaa ccggccggga
    30901 cgttacgccg cacggaaaaa gccagggcaa ccaaaaaatg actcaattta cgcaaaatac
    30961 cgccatgcct tcttccctct ggcaatactg gcgcggcctt tccggctgga acttctattt
    31021 tctggttaag ttcggcctgt tgtgggcggg atatcttaac ttccatccgc tcctcaattt
    31081 ggtgtttgcc gcgtttctgc tgatgcccat tccgcgctac agcctgcatc gcttgcgcca
    31141 ctggattgcc ctgccgatcg gctttgcttt gttctggcat gacacctggt tgcctggccc
    31201 ggaaagcata atgagccagg gttcgcaggt ggcggggttc agtaccgatt atttaatcga
    31261 ccttgtcaca cgctttatta actggcagat gattggggcc atttttgttt tattagtggc
    31321 ctggttattc ctgtcacaat ggattcgcat taccgttttt gtggttgcca tactgctatg
    31381 gctgaacgta cttaccctgg cgggaccaag tttctccttg tggccagccg gacaaccgac
    31441 gaccactgta acaacgacgg gtggtaacgc agcggcaacc gttgcggcga cgggtggcgc
    31501 accggtagtg ggtgatatgc ccgcacaaac tgcaccgcca acaacggcga accttaacgc
    31561 ctggctgaat aatttctata acgcggaggc gaaacgtaaa tcgaccttcc cgtcttcgct
    31621 gcccgctgat gctcagccat ttgaactact ggtgattaac atctgttcgc tttcctggtc
    31681 ggatatagaa gccgccgggt tgatgtcgca tccactgtgg tcgcatttcg atattgagtt
    31741 caagaacttt aactccgcca cctcctacag tggcccggcg gcgatccgtt tactgcgcgc
    31801 cagctgcggg cagacttcgc acactaatct gtatcaaccg gcaaataacg actgctatct
    31861 gtttgataac ctttcgaaac tgggctttac ccagcacctg atgatggggc ataacggcca
    31921 gttcggcggt tttttgaaag aagttcgcga aaatggcggc atgcagactg aattgatgga
    31981 tcaaacaaat ctgccggtta ttttgctggg ctttgatggt tcgccggttt atgacgatac
    32041 cgccgtgctt aaccgctggc tggacgttac cgaaaaagat aaaaacagcc gtagtgccac
    32101 gttctacaac acgcttccac tgcatgacgg caaccattat ccgggggtca gcaaaacagc
    32161 ggattacaaa gcgcgggcgc agaaattctt tgatgaactg gacgccttct ttactgaact
    32221 ggagaaatcg ggtcgtaaag tgatggtggt cgtggtgccg gaacacggcg gcgcgcttaa
    32281 gggcgacaga atgcaggtat ctggcctacg tgatatccct agcccgtcta tcacagacgt
    32341 ccccgttggg gtgaaattct tcggcatgaa ggcaccacat cagggggcac cgattgtcat
    32401 tgaccaaccg agcagcttcc tggctatctc cgatctggtg gttcgcgttc ttgatggcaa
    32461 gattttcacc gaagacaatg ttgactggaa aaaactcacc agtgggttgc cacaaacagc
    32521 accggtctcc gagaactcaa atgcagtagt tattcaatac caggataaac cgtacgttcg
    32581 cctgaacggc ggcgactggg tgccttaccc gcagtaagca cgaaaaaggc cgcagagttt
    32641 aacctctgcg gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatgac
    32701 acgttacttc cgctt
//
LOCUS       DAHBHC010000057        32087 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHBHC010000057 DAHBHC010000000
VERSION     DAHBHC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.057"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..455
                     /locus_tag="M2A35_003150"
     CDS             <1..455
                     /locus_tag="M2A35_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCB7199412.1"
                     /translation="LALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIV
                     NEMVMTGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRT
                     TSEMPVEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            461..1051
                     /gene="caiE"
                     /locus_tag="M2A35_003151"
     CDS             461..1051
                     /gene="caiE"
                     /locus_tag="M2A35_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCB7199413.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1137..1532)
                     /gene="caiF"
                     /locus_tag="M2A35_003152"
     CDS             complement(1137..1532)
                     /gene="caiF"
                     /locus_tag="M2A35_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCB7199414.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(1793..5014)
                     /gene="carB"
                     /locus_tag="M2A35_003153"
     CDS             complement(1793..5014)
                     /gene="carB"
                     /locus_tag="M2A35_003153"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCB7199415.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(5032..6180)
                     /gene="carA"
                     /locus_tag="M2A35_003154"
     CDS             complement(5032..6180)
                     /gene="carA"
                     /locus_tag="M2A35_003154"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCB7199416.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(6636..7457)
                     /gene="dapB"
                     /locus_tag="M2A35_003155"
     CDS             complement(6636..7457)
                     /gene="dapB"
                     /locus_tag="M2A35_003155"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCB7199417.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(7624..8538)
                     /gene="rihC"
                     /locus_tag="M2A35_003156"
     CDS             complement(7624..8538)
                     /gene="rihC"
                     /locus_tag="M2A35_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCB7199418.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(8604..9554)
                     /gene="ispH"
                     /locus_tag="M2A35_003157"
                     /gene_synonym="lytB"
     CDS             complement(8604..9554)
                     /gene="ispH"
                     /locus_tag="M2A35_003157"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCB7199419.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(9556..10005)
                     /gene="fkpB"
                     /locus_tag="M2A35_003158"
     CDS             complement(9556..10005)
                     /gene="fkpB"
                     /locus_tag="M2A35_003158"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCB7199420.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(10093..10587)
                     /gene="lspA"
                     /locus_tag="M2A35_003159"
     CDS             complement(10093..10587)
                     /gene="lspA"
                     /locus_tag="M2A35_003159"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCB7199421.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(10587..13403)
                     /gene="ileS"
                     /locus_tag="M2A35_003160"
     CDS             complement(10587..13403)
                     /gene="ileS"
                     /locus_tag="M2A35_003160"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCB7199422.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(13446..14387)
                     /gene="ribF"
                     /locus_tag="M2A35_003161"
     CDS             complement(13446..14387)
                     /gene="ribF"
                     /locus_tag="M2A35_003161"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCB7199423.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(14395..14613)
                     /locus_tag="M2A35_003162"
     CDS             complement(14395..14613)
                     /locus_tag="M2A35_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCB7199424.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            14716..14979
                     /gene="rpsT"
                     /locus_tag="M2A35_003163"
     CDS             14716..14979
                     /gene="rpsT"
                     /locus_tag="M2A35_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCB7199425.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            15463..16884
                     /gene="espX1"
                     /locus_tag="M2A35_003164"
     CDS             15463..16884
                     /gene="espX1"
                     /locus_tag="M2A35_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HCB7199426.1"
                     /translation="MTNGISTSPHCLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSHNLPKPSPVLHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLD
                     VFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYD
                     HQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQ
                     NFKTSNIAYSQLCKKLSLFKSIPSRLIKKSAYCSTENTITNKFDVVFSYCGNNVKEFI
                     LLLPYNKSLEMHELNEQNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLSNIL
                     CFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHL
                     GYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSS
                     EMRKYLKSINECMFLMKNIAHQN"
     gene            17131..18462
                     /locus_tag="M2A35_003165"
                     /pseudo
     CDS             17131..18462
                     /locus_tag="M2A35_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            18475..19433
                     /locus_tag="M2A35_003166"
                     /pseudo
     CDS             18475..19433
                     /locus_tag="M2A35_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(19472..20371)
                     /gene="nhaR"
                     /locus_tag="M2A35_003167"
     CDS             complement(19472..20371)
                     /gene="nhaR"
                     /locus_tag="M2A35_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCB7199427.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(20437..21603)
                     /gene="nhaA"
                     /locus_tag="M2A35_003168"
     CDS             complement(20437..21603)
                     /gene="nhaA"
                     /locus_tag="M2A35_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCB7199428.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            22189..22341
                     /gene="mokC"
                     /locus_tag="M2A35_003169"
     CDS             22189..22341
                     /gene="mokC"
                     /locus_tag="M2A35_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCB7199429.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(22445..23575)
                     /gene="dnaJ"
                     /locus_tag="M2A35_003170"
     CDS             complement(22445..23575)
                     /gene="dnaJ"
                     /locus_tag="M2A35_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCB7199430.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(23664..25580)
                     /gene="dnaK"
                     /locus_tag="M2A35_003171"
     CDS             complement(23664..25580)
                     /gene="dnaK"
                     /locus_tag="M2A35_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCB7199431.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            25957..26361
                     /locus_tag="M2A35_003172"
     CDS             25957..26361
                     /locus_tag="M2A35_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCB7199432.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            26387..27100
                     /gene="msyB"
                     /locus_tag="M2A35_003173"
     CDS             26387..27100
                     /gene="msyB"
                     /locus_tag="M2A35_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCB7199433.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            27249..27815
                     /gene="satP"
                     /locus_tag="M2A35_003174"
     CDS             27249..27815
                     /gene="satP"
                     /locus_tag="M2A35_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCB7199434.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGPTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(27850..28437)
                     /gene="mog"
                     /locus_tag="M2A35_003175"
     CDS             complement(27850..28437)
                     /gene="mog"
                     /locus_tag="M2A35_003175"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCB7199435.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(28552..29505)
                     /gene="tal"
                     /locus_tag="M2A35_003176"
     CDS             complement(28552..29505)
                     /gene="tal"
                     /locus_tag="M2A35_003176"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCB7199436.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            29784..31214
                     /locus_tag="M2A35_003177"
     CDS             29784..31214
                     /locus_tag="M2A35_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCB7199437.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            31284..32060
                     /gene="yaaA"
                     /locus_tag="M2A35_003178"
     CDS             31284..32060
                     /gene="yaaA"
                     /locus_tag="M2A35_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCB7199438.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         8096 a         8275 c         7660 g         8056 t
ORIGIN      
        1 aactggcgct ggcggcagat tttattgttt gtgccgataa tgccagcttc gccctgccgg
       61 aagccaaact gggcatcgtt cctgacagcg gcggcgtgct gcgtctgccg aagatcctgc
      121 cgcctgccat cgtcaatgaa atggtgatga ccggcagacg aatgggcaca gaagaggcgc
      181 tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat aacgcccgcg
      241 aactggcgca gcagctggtt aacagcgccc cgctggcgat tgcggcgctg aaagagatct
      301 accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc agcggcgtgt
      361 tgaaacacta tccgtcggtt ctgcattcgg aagatgccgt tgaagggccg ctggcgtttg
      421 ccgagaagcg cgatccggta tggaaaggac gttaacaacc gtgagctatt acgcctttga
      481 ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg tcttgattgg
      541 cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc gtggtgacta
      601 cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta
      661 ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcacggag cgatcctgca
      721 tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtgatta tggatggcgc
      781 ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcag gctttcacgg
      841 cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg ttagtgacga
      901 cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg ggcgctgtca
      961 tgcatcgtta catgaaacac agccactgag gcaaatggag gaaaatcgcc cccgtttgca
     1021 ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg atgcgacctg gttgctggat
     1081 aagatgctac aacatcgcat ctggcaagca ggacgcccgg tatcaacgag ataaaattaa
     1141 cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag ttccgtgagc
     1201 ggaacggcag gaatacgcgg cgtctttctt acgccgacta atttatcccg gctgttattg
     1261 cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt ttaccagtcg ctggcactgg
     1321 catccccgtc cttccagctt attagggatc atcttaactt cgcagcttat ttctgcgact
     1381 tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc gaaatgaata
     1441 gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc gattaacaag
     1501 tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca aattaaaagt
     1561 ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt ggatatatcg
     1621 atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt catcaaaaat
     1681 atcgattccc caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt
     1741 attacttatt cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga
     1801 tctgcgcgtg catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca
     1861 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
     1921 tgcgacggat cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt
     1981 aggtatattc gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt
     2041 tcaccagacg cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
     2101 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
     2161 cttcgcgcac ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg ctgcccagct
     2221 gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc
     2281 gcatttctgg ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt
     2341 ctttcaccga gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt
     2401 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga
     2461 acggaacggt acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca
     2521 ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct
     2581 gctggcgcat cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg
     2641 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
     2701 tttcgccgtc gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca
     2761 gcaacactgg cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag
     2821 cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgtaccacca
     2881 gcgggtagcc aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt
     2941 tcgccggttg tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg
     3001 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc
     3061 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
     3121 tacgcacgat ttctagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
     3181 tgtcgtagtc ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
     3241 cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga
     3301 tacggttcgg gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt
     3361 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac
     3421 aggtatccac gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt
     3481 ccgcttcgcg cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca
     3541 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca
     3601 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca
     3661 cgccgtccac agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc
     3721 cagcatcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt
     3781 tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct
     3841 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
     3901 cgttagcacc agcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga
     3961 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga
     4021 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
     4081 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
     4141 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
     4201 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
     4261 gcgcgacagt gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc
     4321 agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc
     4381 cgatcagcga ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc
     4441 aaatttcttc aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa
     4501 aggatgggcg aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca
     4561 tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
     4621 aacgacggcg gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc
     4681 cgaactcttc caacacgccc tgacgctcca gctccagcgc gcagttcagc gccgtctgac
     4741 cgcccatcgt tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt
     4801 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
     4861 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
     4921 gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa
     4981 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
     5041 ggttttacgg tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg
     5101 tggcccaggg ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg
     5161 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
     5221 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
     5281 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
     5341 accgctcgcc agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt
     5401 ttcgaggaat ttctggatgg cagtaatggc gtaatcgcac ggggccgggt cgccaggacc
     5461 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
     5521 cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc
     5581 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
     5641 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
     5701 ttttgccaga tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc
     5761 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
     5821 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
     5881 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcagat cgcgaatcac
     5941 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
     6001 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
     6061 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
     6121 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
     6181 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta
     6241 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
     6301 ggcgctaaaa gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt
     6361 ttacaggcat taagcgacaa aaagatcata taacctgttc tggaaaacgc ttgcgcagca
     6421 agaagattgc atgaaataaa agcgagcagg gaaaagtgga ccaaatggtc aaaatttaca
     6481 ttaaaacaac aaaaaccgaa gacaaaacat gtaatttttg gcacttatag acaatttaaa
     6541 aacataacat taaaaataaa agggccaaaa attaaagccc tttataatca ataaattaaa
     6601 tgtgttattt ttgcaccata acaaatattt tgtggttaca aactattgag atcaagcaca
     6661 tctcgcatat caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgcg
     6721 ccgttagcaa atgtcatgcg gctggacgcc ttatgagtga tctccagacg ctcgccaata
     6781 tcggcaaaca tcgcggtatg ttcaccaacg atgtcacctg cacgcacggt ggcaaaacca
     6841 atggtgccag gcacacgttc accggtgtgg ccttcacgac tgtagaccgc gcaatctttc
     6901 agatctttat caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc
     6961 gcatcaactt tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc
     7021 actttggctg ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg
     7081 gcaaagacaa tcgcaatatc ggcggcggcg tcacgaatcg cctgtttacc ggcttcgtca
     7141 aaccccgtag tgccgatcac catccctttg ccatgctggc gacaaaaagc gagatggttc
     7201 agcgtacctt ccggacgggt aaaatcgata aacacatcaa aatcatcttt taccgcatcg
     7261 aggctgcttt gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg
     7321 cccagtaaag aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc
     7381 agcgccgcct gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg
     7441 atgtttgcat catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta
     7501 accagcgcat cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca
     7561 gggagggata tcagtgaaag gcatgagtcg atgaatgact gcatgccaat aacatgtgac
     7621 aggttacgac gccagagcca gcacctcagc cacccactgc tggaagcctt tcacatccag
     7681 atccaatgcc acctgtacat tggctggctt gcccaggcaa ccgtcgatat caaccaccgt
     7741 cgtgcctgag gtaaattcgc cctgagtttc cactgccaca aaacagggtt tgagagtgaa
     7801 caggtccggg cgcaccagcc aggcgatggc gcagagatcg tgcattcgca gaccgctttg
     7861 catactgccg ctacggtagt ggctaaacag tgcatgaagc atttttccgg tgcgattcaa
     7921 ctccggcaaa gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc
     7981 gcacatgacg atttcgattc cgctacggaa gacacaggca gccgcttctg gatcggcagc
     8041 gatattaaac tcggcgtttg gcgtacagtt gccgcgtccg gcagaaccac ccatgatcac
     8101 cagacggcga atatacggct tgcattccgg gcattgtgaa agtaacagcg caatattggt
     8161 taacgggccg atggccacca gggtaacagg ctctggtgca cgcatcaggg catcccgaat
     8221 cgccagaaac gccggtatcc cgagcggctt tcggttgtgc tcaacaaagt cgtagccagc
     8281 cattcccgat tcgccgtgca cagatgccgc atcacgcggt gcgcgtacca gcggcacagc
     8341 ggccccttgg gcgagcggaa tctccgcatt ccagaaatgc agcagttgca aggcattacg
     8401 ggtagttttc tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc
     8461 gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag
     8521 gaagataggt aaacgcatgt tttctccata aaaaatgccg gtaacaagac cggcattttc
     8581 gcataactta ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc
     8641 ggcacttcga aaacaatgtt ttcttcacgg ccttccagcg gaatggcttc accaccgccc
     8701 agctgctgca aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca
     8761 gtcacgccga cgcatttaac ctctttcacc cactcttcct ggatgtcttt cgcatcgtca
     8821 atcaaaaacg cgcgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag
     8881 gagtttttcg aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct
     8941 tcctgacggt tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg
     9001 aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtctgg
     9061 gtcataaagg agagcttctc ttcgtttttg accgtcagtt tccacacatc gtccggcgat
     9121 tcgaccagat acattccccc ttccgggtta ctgtactggc ccattgtccc ttccacttcc
     9181 gggtgcccgg cgtgaccgat gagaatagat tcttcgccac ggcgactggc gcgggcgact
     9241 tccatatgca ctttggtcac cagcggacag gtcgcgtcaa ataccgtcaa atcgcgactt
     9301 tttgcttcgt tacgtaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg
     9361 tccggtactt cgctaatctg ctcaataaag atagccccac gctcacgcaa gctatcgacc
     9421 acatagcggt tatgtaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc
     9481 gcgttttcaa caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc
     9541 aacaggatct gcatgttacg cctccagtgc cggatcgatt tccagcactt caatatcaaa
     9601 atgaacggtc tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat
     9661 ttcgcggatc acgccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc
     9721 aatttctggc tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg
     9781 tgacggcacg ccaaacgccg catctggctc caacgagaag gtggttttat cgcccacttt
     9841 cagccccaac aggtgttgct ccagcccttc agaaagagaa gcatcaccca ggcggaacag
     9901 cgccggttta ccgttgttgc gggtagactc ggcggtggtg ccatcgtcga gttttagcgt
     9961 gaagtgcacc aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta
    10021 tattttgagg atttttgccg gatgcggcct acgatttcgc agcaatctgt aggccttatc
    10081 cggcagggtt tattattgtt ttttcgcttt agaaggcaaa aaaccttcca gcacaatcag
    10141 tgccgcaccg acacaaatgg cagtatcggc aaggttgaag gtggcgaagt gccagtcgcc
    10201 gacgtagaag tcgatcatat cgacaacgaa gccgtgccac aggcggtcga acaggttgcc
    10261 cagcgcgccg ccaataatca gcgcataagc gatattgttt agcttctgcg tggccttcga
    10321 gcgatacatc atcactgcca ggatcacgct aataccaatc gcaataccgg caaagaacca
    10381 acgctgccag ccgccgctat cggcaaggaa actaaacgcc gcgccatagt tacgcgcata
    10441 atgcagatta agcgacggga acagcgggac cgtatccccc agagcaaagt tctggaggat
    10501 caggtatttg ctgcccagat cgataatcag cacgactacc accagccaca gccagcgtag
    10561 ccctgttgaa cagatcgatt gactcatcag gcaaacttac gtttttcacc gtcaccggcg
    10621 acgttgctga cacagcggcc gcagatttct gcgtgttccg ccaccttgcc gacatcctgg
    10681 gtgtagtgcc agcagcgtgg gcacttctca ccttcggctt tactcaacgc gactttcagc
    10741 cctttgagta cttcgctctg ctgagcatca gcaggtgcgt cgttatagtc tgcaacggta
    10801 gcgccggagg tcaacaggac aaatcgtaat tcatcgccca gcgcggtcag tttcgccgac
    10861 agttccggtt ctgcatacaa ggttactgcc gcttccagcg agccacccac tttcttgtcg
    10921 gcacgcgctt gctcaatgac tttgttcact tcgccacgca ctttcaacag ctcgtcccag
    10981 aacgcatcgt tcatcgcttc actgtctgcc aggccaaaca ggccttcgta ccactcaccg
    11041 gtgaagacgt atttttcacg ttcgcccggc aggtagcccc acacttcatc agcggtgaag
    11101 gagaggattg gtgccatcca gcgcaccagc gcttctgcga tgtgatacag cgcagtctgg
    11161 cagctacgac gcgccacact gtccgctttg gcggtgtact gacggtcttt gatgatgtcg
    11221 aggtagaagg aacccatctc aacggagcag aagcgcatca gacgctgtac cacttcgtgg
    11281 aaatcgtatg cttcgtacgc cttgaggatg tcttcctgtg ccgctttcgc acaacctacg
    11341 gcccagcgat ccagtaccac catctcttcc ggtttcacca tatcttttgc tggatcaaaa
    11401 ccgttcaggt ttgccagcag gaagcgcgcg gtgttacgga tacgacgata gctatcggca
    11461 gcacgtttca ggatctcgtc agaaacggcc atttcaccgg tgtagtcggt tgatgccacc
    11521 cacagtcgca gaatatccgc gcccagtttg ttcatcacat cctgcggcga aacggtattg
    11581 ccgatggatt tagacatctt gcgaccctga ccatccacgg taaagccgtg ggtcagtacc
    11641 tgacgatacg gcgctttgcc tttcatcgcg gtggagatca ttagggaaga catgaaccaa
    11701 ccacggtgct ggtcagaacc ttccagatac atgtccgctg cgtgaccggc aaattccgga
    11761 cgcacgtcaa caacagaaga gtgggtagat ccggagtcaa accatacatc caatgtgtcc
    11821 ggcactttca cgtactgatc agcttcatcg ccgaggatct ctttcgcatc aagatcccac
    11881 cacgcctgga tgccgtcaac ttcaacgcgt tttgccactt cttccattag ttcgagtgta
    11941 cgcggatgca gctcttccgt gtctttgtgc acgaacagtg acatcggtac gccccaggtg
    12001 cgctgacggg agatacacca gtcaggacgg ttagcaacca tcgactcgat acgcgcctgg
    12061 ccccagtccg ggatccactg cacgcctttg atctctttca gtgactgcgc acgcagacct
    12121 ttctgatcca tgctgacgaa ccactgcggc gtcgcgcgga agatgatcgg cgttttgtga
    12181 cgccagcagc acggatagct gtgctgcatt ttctcaacgt gcagcagcgc gcctttttcc
    12241 tgcagcagcg caacgacgat gtcgttcgct ttgaagacgt tcacgccatc cagcgtcgga
    12301 taagtgcccg gcagataagt gccgtccggg ccaaccgggt tagcggtttc caggccgtat
    12361 ttctgaccga tcacatagtc gtccgggccg tggccaggcg cggtgtgaac ggcaccggta
    12421 ccggcatcca gggtaacgtg atcgccgagg attgccggaa cgtcgaagcc cataaacgga
    12481 tgggtaaagc gcagcaactc aagctccgca ccttttaccg tgccgagaat ggtgtaatcg
    12541 gtcacgccga tacgctgcat tacgctttca accagatctt tcgccagaat cacggcctga
    12601 ccgtcgatct gcaccagcgc atagtcgaaa tcaggtgcaa tagagattgc gcggttcgca
    12661 ggcagagtcc acggcgtagt ggtccagatc accagcgaga ttgggccgtt aacgttgctt
    12721 acggcaaatt ttgctttcag tgcatcctga tcgaccgcct gaaaagcaac gtcgatggac
    12781 ggagaagttt tgtcgtaata ctcaacttcc gcttccgcca gcgcagaacg gcagtcaacg
    12841 caccagtgaa ctggcttcgc gcctttgtgc aggtgaccgt tgccgatgat tttgcccagc
    12901 gcgcggatga tgttggcttc agttttgaag tccatggtca ggtacgggtg cgaccagtcg
    12961 cccagcacgc ccagacggat aaagtctttg cgttgaccgt caacctgggt cgccgcgtat
    13021 tcgcggcact tggcgcggaa ctcggcggcg gtgaatttct cacccggctt accgtattct
    13081 tgctctactt tcagctcgat cggcagaccg tggcagtccc agccaggcac atacggcgag
    13141 tcataaccgg aaagcccttt ggacttcacg ataatgtctt tcagaatctt gttaaccgag
    13201 tgaccaatat gaatgctgcc attcgcataa ggagggccat catgcagaat gaaggttttt
    13261 ttgccttttt tagccgcacg gatgatgccg tacagatcat catcagtcca acgcgccagc
    13321 attcccggtt cgcgcttggc gagatcgcca cgcatcggga accctgtttc cggcaaattc
    13381 agggttgatt tatagtcact catcagattc tcggttccgt gtttcggttt gattacataa
    13441 caggcttaag ccggttttgt tagcccaaaa aattcgcggg cggttaattc atcacgcgca
    13501 atctgcgctt tcagttcgtc cagcgacgca aatcgctgct cattgcgtat ttttttacgc
    13561 agcactactt gtatatggcg accgtaaagg tccattgcaa catctaacaa atgcacttcc
    13621 agctgctggc gaataccggc aaccgttggg cgtgttccaa tgtttgccac gccgggtaac
    13681 ggcttttcac cgaggcccag cacttctacc gcataaaccc ctttcaccgg ggaaacctga
    13741 cggcgtagcg gtacattcgc cgtcgggaaa cctatagtgc gccctaattc atcaccgtgg
    13801 actacacgcc cggagatggc aaacgggtgc cccagtaaac tttctgccag agccagattg
    13861 tcatccgcca gcgcctgacg cacagcggtg ctgctgatac gcacgccacc ttcgcaaaaa
    13921 gtttgcgtac tggtgatatc gaagccgtat tccatgccag ctttctgtaa taacaagaaa
    13981 tcgccttcac gaccagcgcc aaagcggaaa tcatcaccta cggcaagaaa ttttacgcgc
    14041 aaatgcttca ccagaagatc gctgatgaaa ttttgcgcgg ttaacgccgc gaaacgcctg
    14101 tcgaaacgca cgcacagcac gtaatcaacg ccacactctg caaggtaacg cagtttttcc
    14161 cgcagccggg tcagccgtgc cggggcttta tcggtagcaa acagttccag tggttgaggt
    14221 tcaaaaagca tcaccatcac cggtaagttg cgcttgcgcc cttcttcctg caagccctgt
    14281 aacagcgcgc gatgaccgcg atgcacgccg tcgaaattac caatagtcag cacacaccct
    14341 tcttgcgggg cctggctgag attatgtatg ccgcgtatca gcttcatgtc tggctcaaaa
    14401 cagtgaaaat cgtccgagta taccttgtac agcggtcaag gttaaccggc gattgagtac
    14461 cgaaatcaga aagaagtggc ttttcattgc catggcgcaa atcacgggaa gaaagtgacc
    14521 gcctgctgca atttttatcg cggaaaagct gtattcacac cccgcaagct ggtagaatcc
    14581 tgcgccatca ctacgtaacg agtgccggca cattaacggc gcttatttgc acaaatccat
    14641 tgacaaaaga aggctaaaag ggcatattcc tcggcctttg aattgtccat atagaacaca
    14701 tttgggagtt ggaccttggc taatatcaaa tcagctaaga agcgcgccat tcagtctgaa
    14761 aaggctcgta agcacaacgc aagccgtcgc tctatgatgc gtactttcat caagaaagta
    14821 tacgcagcta tcgaagctgg cgacaaagct gctgcacaga aagcatttaa cgaaatgcaa
    14881 ccgatcgtgg accgtcaggc tgctaaaggt ctgatccaca aaaacaaagc tgcacgtcat
    14941 aaggctaacc tgactgcaca gatcaacaaa ctggcttaat cgccaatttg ctgaagcttt
    15001 gtgaaaaagc ccgcgcaagc gggttttttt atgcctgctg cttttatgat gcgtgagaga
    15061 acacactacc ggttgccaga tagtcaaaag atgctggctt ttgcagacat gccgttacac
    15121 tttctgaact cttcgtggct taagaatcgt agaaccatcg gctgatacat tctgaacaag
    15181 aatatctact caatctccag actcccctac aaagaatgtg gatattgtga tacacattga
    15241 ggtattaaac ttaagaccac tctatttttg aattaaattc ctatacactt taaccattca
    15301 taaatatctc cttattagat cttgtaaata attaacaaca atcaacacag gttaaatacc
    15361 tatttatatt ctacttcata aaattaatga gctatttttg atagtagtca cattcactca
    15421 taaattaaat taataaaaat tcatcaactt caaggactga taatgacaaa tggtatctca
    15481 acttcgccac attgtcttta taaatcaaat atagtagatg atgtaataat aaataaaaca
    15541 agacaaaatg agttagtaaa agtattttgc gagtataaaa cagagttctt aattttgttt
    15601 gatgactttt ttagaagcca taacttgcct aaaccatctc cagtccttca tcattttttt
    15661 caatatactc atctgagaga tgcatatttt tatagatgta aactcataga acatacagtt
    15721 caattttatt tttttaaaca caaaggtatc acattactta gattggatgt ttttgatgac
    15781 agaacaagcg aatgtctttc tgaagagatt aagatatacc aagaatgcca tgaaaaattc
    15841 gttaattttt taaaggcaaa cttcaaccaa gaaatatacc cacagctata tactcctgaa
    15901 atattttatg aggcctgcag aaatctccag tcattttatg atcatcagga aacgtcacaa
    15961 aacgcaaaat acagtgcaat agacaagaaa aaaagtcatc ttaaccagga aatcaaaaag
    16021 cttattaaaa aaaatatttt ccctgagcta tacaatgagc aatgcaataa aatcccctcc
    16081 tcatcgatag atgataacca gggaataatg tggcagaact ttaaaactag caatattgca
    16141 tattcacaac tatgtaaaaa attatcttta tttaaaagca tacccagtcg attaataaaa
    16201 aaatcagctt actgctcaac tgaaaacaca atcaccaata aatttgatgt tgtcttttct
    16261 tactgcggaa ataatgtaaa ggaatttata cttttattac cctataataa atctctggag
    16321 atgcatgaac ttaacgagca aaacatccag tatttgactg cccttaatat taatatacat
    16381 aaaatgttgc tatcaaatat aacaatagaa aagagcgacc tttcatatgg gtattatttt
    16441 ggttgtgtat tatccaatat attatgtttt gaaagcgact tatcaaacac catcttttca
    16501 aatggagaaa ttaataatct cttcattaaa aaaagcaaca tatttggagc atcattcact
    16561 aataccagaa taaaaaattt gctctgtgaa gatattatgc ctggtcgatg gaccactcaa
    16621 cttgtcaata agcacttagg ttacagatac acaggagtat tcaaaaccct ggcaagtatt
    16681 gatgacaaac cgtcccgttt cgaaattttg ataccattgg tacaaacttt agttcgcgac
    16741 aatgtaaagc taaataatga tgtgtataaa gaacttaata agtttatgca cgattacgac
    16801 aaaacatcat cagaaatgag aaaatacctt aaaagcatca acgagtgtat gttccttatg
    16861 aaaaacatag ctcatcaaaa ttaaaaaatc tcaaaaaaga agtaatttca gaatgaaatt
    16921 aactgtgatg attattaatt cactacagag gaataaaaaa taatctgaag catactctga
    16981 aacatcctac tctctcactc taataatggc accttgctct tcttataaag aagggaataa
    17041 ttaagaatat taaaccttca tcaacttcac atcaagaaat taaaaatttt aaacattaat
    17101 ctaaataata acaacctgat cacataatcg ggtacgattt tatccgataa ctataatgct
    17161 ataactctgg gaaatggttg gaatacaccg ctgggtgcca tctcttttta cgctgcccga
    17221 tcgagtagta agttaaataa tgataccaga cacgaaggta cgagctatca ggtcgcctat
    17281 aataaatatc tagtacaaac agccactcga tttagcgtgg ccgcctggcg ttatgcttcg
    17341 caggattaca ggacattcag cgaccatctt tacgaaaatg ataaacacta tcatcagagt
    17401 gattatgatg atttttatga tattggcaga aaaaatagcc tttctgctaa tattatgcaa
    17461 cctttatcca ataatctggg aaatgtatca ttaagtgcgc tttggcggaa ttactggggg
    17521 cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca acacattagt
    17581 tatactttct ctgccagcca atcttatgat gaaaataata aagaagagga gcgttttaat
    17641 ctgtttatct ccattccttt ctactggggg gatgatattg ccaaaacacg tcaccaaatt
    17701 aacttatcga attcgacctc attttccaaa gatggctatt cccccaacaa tactggaatt
    17761 actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa tcagcaacaa
    17821 caaaataatg atacctcgct tggtacgaat ttaagctgga gaactcccat cgccataata
    17881 gatggcagct atagtcattc taaaaacgcc tggcaaagtg gtggaagtat tagtagtgga
    17941 ttagttgtct ggcccggtgg tattaatatc actaaccagt tatccgatac atttgcaatt
    18001 ctggatgcgt ctgggttaga aggtgcacat attaatggac aaaaatacaa tcgaacaaac
    18061 agcaaaggcc aggttgttta cgacctgata atacctcatc gtgaaaacca tctggtactt
    18121 gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat tattgcacct
    18181 taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata
    18241 caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact ggatctccag
    18301 gaaaataata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt
    18361 cccactggaa taaaagttgc tctcaatgat gaacagaact tattctgtac tattactttt
    18421 caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag
    18481 tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc tccctgtgca
    18541 ttgaccaatg tcggtgaaca acgtggaaca tatatactca agccactgag tatgaaaggt
    18601 aatctaaccg ccgaaaaatt gaaaacaccg gaaatatcat tgagtgtaca gacttgcaag
    18661 agacaaccaa cgccattaaa gttcattttt taacacagaa cgctttaagg tttaaagtcg
    18721 gaagtcgtca ttatattatt aatttcccct ttgaatctga cggtaaaaaa atagagcttc
    18781 cagggaagtc atcttataat ttagaagaaa tgctatctgc gataaatttc accattccat
    18841 attctatcgc atctgtatca gatagttcta acaccgcaga catttttcct ggaactcctt
    18901 ttcctttgtt gttcgaaatt gaagtgagtt catgtacagg gaataatact aattgtaata
    18961 gcgtccgctt taattataat ttaaatacta ttttacaggt aaatttaatg acctgtggtt
    19021 ttgaagatca atccatcgac actggacgat ttgtactgtc tcgtatcaat aatgaaaatt
    19081 atcagtttca tcatataaga tttgactgca tccagggcga gcaaggtgaa ttagtttttg
    19141 aacccagtga tgttgaatat tactttgaac cagtgacgat acaggagtca ggaaccagca
    19201 tattaaaaaa tgagttagaa aacagtgagg acggtgcggg tcaaataggc tttcaactct
    19261 ccaatgatgg tacgaatgaa atccagtatg gtaaaagcaa ttactactct tttcagcatc
    19321 cccatgaagg aagtaatcag attcctctat ttattcgccc gcgtacatat ggaaataatg
    19381 tctcaagcgg gcaaataatg agccgagtca aaatcgtggt tatgtacaac taatctatct
    19441 cccccacctt aactttgggg ggaggtgccg attaacgcgc cgctggacta aaaagcgcag
    19501 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
    19561 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
    19621 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
    19681 aagctttcat caacgcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
    19741 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
    19801 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
    19861 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
    19921 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
    19981 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
    20041 cgctactgac caggcgtttg gaaagtgcat cagccacacc aacgtcaaac aataaattgg
    20101 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
    20161 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
    20221 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
    20281 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
    20341 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
    20401 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
    20461 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
    20521 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
    20581 catagtaaaa ccgataccgc acaggatccc caccgccata atttgctgat aagtcgttcc
    20641 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
    20701 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
    20761 cagcgtgaca ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
    20821 atacgccacc cacgggtgta acacatgctc cagtcgcttc gctggagaac gcccatgctt
    20881 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agggttgcgt gaacccccga
    20941 tttcaacacc gcagtccaca acactacgcc aaccagaata tagacgcccg tgcggcgtac
    21001 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacac caagagaggc
    21061 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
    21121 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
    21181 cagcacacca agtgcaaagg caatgtcagt agccgccggg atcgcccacc cttcgcgggt
    21241 aatcggatcg gcatagttaa aggccagata gagtaatgcc ggaacaatca tcccaccaat
    21301 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
    21361 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
    21421 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
    21481 aaagtcgtga taccatccac tggttgcgcc gctattggcc ataatcatcg ccaggatagc
    21541 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
    21601 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacaaatc
    21661 atcgttaaaa tcagattata gtgtaggaat aactcgtttt ttacgatcca ttgaagaatt
    21721 tgcgccatca gcggtgagat gagtttttga cggctgtaag cgctcacgag tgaccctaag
    21781 cccagataat catagcttaa ggtgaggatt tctgagagat taattaggaa aagtaataat
    21841 taatttgttg tcagtcggcg atgtttgtta ggccaatatt tcaaaaataa tccagcttgt
    21901 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
    21961 cgccataaaa atgcgaggcg tcttcaggat tagaatcgat aaaacagaat gggttaaaga
    22021 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
    22081 gatagagaaa agccccgagt gatattttac catcaacccg aggcccccta tatgctgaac
    22141 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
    22201 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
    22261 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
    22321 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
    22381 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
    22441 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
    22501 gcgggctgtt gtgctcaccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
    22561 gtttctgctt ctcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
    22621 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaaa agcttaccgg
    22681 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
    22741 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatagagg ttgttgcctt
    22801 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
    22861 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
    22921 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
    22981 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
    23041 cagcgaagaa tccctgacgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
    23101 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
    23161 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
    23221 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
    23281 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaaccg ccgccgccca
    23341 taccaccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
    23401 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
    23461 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ttgatttcac
    23521 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
    23581 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
    23641 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
    23701 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
    23761 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ctcctgcatt
    23821 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagtgca
    23881 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
    23941 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
    24001 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
    24061 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
    24121 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
    24181 cgcggtgccg ggttgatacc atccaggttg aactgaccca gagatttgtt atcagccgca
    24241 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
    24301 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
    24361 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
    24421 ttcacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
    24481 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
    24541 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
    24601 gcgtcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
    24661 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcatctgca
    24721 gtgatgtacg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
    24781 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
    24841 tctttcttga attcttcaac caggtagttg atcagacggc tgtcgaagtc ttcaccaccc
    24901 aggtgggtat caccgttggt agccagaact tcgaaggttt tttcgccgtc aacttcgtcg
    24961 atttcgataa tagaaatatc gaaagtacca ccacccaggt cataaaccgc gatagtacgg
    25021 ttgccagtac ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
    25081 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
    25141 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccaa gtaatcttca
    25201 gcggttttct tcattttttt cagcacttca gcagagatct gcggcggtgc cattttctgg
    25261 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
    25321 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
    25381 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
    25441 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
    25501 agcacgcgag gagtggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
    25561 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
    25621 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
    25681 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
    25741 agaaattttt tgtgcttttg cgcagaaatt tcgcgatata gcaggacaag acgtgaaact
    25801 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa gacattcatc aaaaaattgt
    25861 aataaaaaga aaacattacg cacctgaatc ttctctttat cagcagtaaa ctagtgggta
    25921 tccatccccc tacctctccc cactaagaga atccttatga aatccgtttt tacgatttcc
    25981 gccagcctgg cgattagtct gatgctgtgc tgcacggcgc aggcaaacga ccataaaatc
    26041 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgttgaa acttcctgtt
    26101 tgccgaattg ttaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
    26161 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
    26221 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
    26281 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
    26341 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
    26401 cctgaatgat tcagatctgg attttctcca gcattgcagt gaggaacagt tggcaaattt
    26461 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
    26521 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
    26581 gattgctgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
    26641 cggtaaattg tatcgggcca ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga
    26701 caaagagatg tctacgtttg agattgagca acagttactg gaacaatttc tgcgtaacac
    26761 ctggaagaat atggacgagg aacataagca ggagtttctg cacgcgatcg atgccagggt
    26821 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggcgt
    26881 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
    26941 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtaggtg cggcactaaa
    27001 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
    27061 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
    27121 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
    27181 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
    27241 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
    27301 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
    27361 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
    27421 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
    27481 cgctggttgc gattctgctg atgccgaaac tgggtccgac cgatgcgcca aatgcacagt
    27541 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcacgc
    27601 tgaaaggcgc acgcgttctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
    27661 tggcgatcgg taacattgcc ggtaacgccg caatcatcca ctttgccggc tggattgggc
    27721 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtactgaac gagcagtttg
    27781 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtt ttgagatccc cccgctcggg
    27841 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg ctgcaaccac
    27901 ttccggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
    27961 aaaaataccg tgtaccacaa cgttacccgc agcgtccttc acaccttcca gcgtctcttt
    28021 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
    28081 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
    28141 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
    28201 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccactagctc
    28261 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
    28321 cggcgtggtt agcgccgatg tcagccattc ttccagcgca gggatgcctt tatcctgata
    28381 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
    28441 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
    28501 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
    28561 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
    28621 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
    28681 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
    28741 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcgc agcctgccag
    28801 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accactgttt cataaccgtg
    28861 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
    28921 gtactctttc ttatcagtat tcgctttgta ccagtcaaga atacggccaa caaatggcga
    28981 gatcaggaac acgcctgctt ccgcacaagc acgagcctga gcgaaggaga acagcagggt
    29041 caggttacag ttgatgcctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    29101 agaagccagt ttgatcagaa tacgatcgtt gctaatacca gcatcgttgt agagtttgat
    29161 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    29221 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    29281 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaagcgac
    29341 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    29401 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    29461 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    29521 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    29581 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    29641 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    29701 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    29761 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    29821 ggggatcagt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    29881 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    29941 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    30001 gtagcggcaa cctggccggc gttgcgctgg ctattaccgc cggtggacct ggagccgtct
    30061 tttggatgtg ggttgccgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    30121 cacaacttta taaagaacgt gacgtcaatg gacagtttcg tggaggaccg gcatggtata
    30181 tggcgcgcgg gctggggatg cgttggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    30241 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    30301 cttttgattt tcccccgctg gtgacaggca ttattctcgc tgtctttgct ctgctggcaa
    30361 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg ttttgtcccg ttgatggcga
    30421 taatctgggt actgaccagc ctggtgattt gcgtaatgaa tatcgggcaa cttccccacg
    30481 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    30541 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    30601 cgggaatggg ttctacacca aacgcagcag cggcagctgc gtcctggcct ccgcatccgg
    30661 cagcgcaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    30721 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    30781 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta
    30841 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    30901 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    30961 ccttcgcaac ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag
    31021 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    31081 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    31141 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    31201 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    31261 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    31321 accaaagccc gttgaccacc atgcgctata cgctgccgga gctgttagac aattcccagc
    31381 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    31441 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    31501 cggagaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    31561 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    31621 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    31681 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    31741 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    31801 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    31861 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    31921 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    31981 ttaatagcga aggttacttc tttgatgaag cttcctccag caatggtgaa ctggtgttta
    32041 aacgctacga gcagcgttaa gtttgtagcc aatgccggat gcggcgt
//
LOCUS       DAHBHC010000058        31750 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHBHC010000058 DAHBHC010000000
VERSION     DAHBHC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.058"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            503..883
                     /locus_tag="M2A35_003179"
     CDS             503..883
                     /locus_tag="M2A35_003179"
                     /inference="COORDINATES: protein motif:HMM:NF022337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCB7199439.1"
                     /translation="MTNRERYFFNLLYGQFSHNYFLFSQVRMVDLITPVFDKDTQYNS
                     FIRAFRSISQYHIDFLIVSRIDFSIVCAIELDDSSHDLASRVKRDNKINLIFKFIGIP
                     LFRSRNPLALLEMVKKHIDFQNVN"
     gene            1065..1409
                     /locus_tag="M2A35_003180"
     CDS             1065..1409
                     /locus_tag="M2A35_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCB7199440.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            1771..2130
                     /locus_tag="M2A35_003181"
     CDS             1771..2130
                     /locus_tag="M2A35_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCB7199441.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(2250..2429)
                     /locus_tag="M2A35_003182"
     CDS             complement(2250..2429)
                     /locus_tag="M2A35_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCB7199442.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            2503..3864
                     /gene="pabB"
                     /locus_tag="M2A35_003183"
     CDS             2503..3864
                     /gene="pabB"
                     /locus_tag="M2A35_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCB7199443.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            3868..4446
                     /locus_tag="M2A35_003184"
     CDS             3868..4446
                     /locus_tag="M2A35_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCB7199444.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            4630..5994
                     /gene="sdaA"
                     /locus_tag="M2A35_003185"
     CDS             4630..5994
                     /gene="sdaA"
                     /locus_tag="M2A35_003185"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCB7199445.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            6125..7723
                     /locus_tag="M2A35_003186"
     CDS             6125..7723
                     /locus_tag="M2A35_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCB7199446.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGAMALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(7727..9283)
                     /gene="yoaE"
                     /locus_tag="M2A35_003187"
     CDS             complement(7727..9283)
                     /gene="yoaE"
                     /locus_tag="M2A35_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCB7199447.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLITVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            9746..10717
                     /gene="manX"
                     /locus_tag="M2A35_003188"
     CDS             9746..10717
                     /gene="manX"
                     /locus_tag="M2A35_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCB7199448.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            10780..11580
                     /gene="manY"
                     /locus_tag="M2A35_003189"
     CDS             10780..11580
                     /gene="manY"
                     /locus_tag="M2A35_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCB7199449.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            11593..12444
                     /gene="manZ"
                     /locus_tag="M2A35_003190"
     CDS             11593..12444
                     /gene="manZ"
                     /locus_tag="M2A35_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCB7199450.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            12499..12957
                     /locus_tag="M2A35_003191"
     CDS             12499..12957
                     /locus_tag="M2A35_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCB7199451.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            13386..13952
                     /gene="mntP"
                     /locus_tag="M2A35_003192"
     CDS             13386..13952
                     /gene="mntP"
                     /locus_tag="M2A35_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCB7199452.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(13949..14758)
                     /gene="rlmA"
                     /locus_tag="M2A35_003193"
     CDS             complement(13949..14758)
                     /gene="rlmA"
                     /locus_tag="M2A35_003193"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCB7199453.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(14924..15133)
                     /gene="cspE"
                     /locus_tag="M2A35_003194"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(14924..15133)
                     /gene="cspE"
                     /locus_tag="M2A35_003194"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCB7199454.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(15146..15289)
                     /locus_tag="M2A35_003195"
     CDS             complement(15146..15289)
                     /locus_tag="M2A35_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCB7199455.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(15959..16246)
                     /locus_tag="M2A35_003196"
     CDS             complement(15959..16246)
                     /locus_tag="M2A35_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCB7199456.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(16321..16464)
                     /gene="mgrB"
                     /locus_tag="M2A35_003197"
     CDS             complement(16321..16464)
                     /gene="mgrB"
                     /locus_tag="M2A35_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCB7199457.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            16623..16862
                     /locus_tag="M2A35_003198"
     CDS             16623..16862
                     /locus_tag="M2A35_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199458.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(17005..17796)
                     /gene="kdgR"
                     /locus_tag="M2A35_003199"
     CDS             complement(17005..17796)
                     /gene="kdgR"
                     /locus_tag="M2A35_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCB7199459.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSLRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            17973..19346
                     /locus_tag="M2A35_003200"
     CDS             17973..19346
                     /locus_tag="M2A35_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199460.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(19392..20273)
                     /gene="htpX"
                     /locus_tag="M2A35_003201"
     CDS             complement(19392..20273)
                     /gene="htpX"
                     /locus_tag="M2A35_003201"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCB7199461.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(20465..22513)
                     /gene="prc"
                     /locus_tag="M2A35_003202"
     CDS             complement(20465..22513)
                     /gene="prc"
                     /locus_tag="M2A35_003202"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCB7199462.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(22533..23231)
                     /gene="proQ"
                     /locus_tag="M2A35_003203"
     CDS             complement(22533..23231)
                     /gene="proQ"
                     /locus_tag="M2A35_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCB7199463.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(23328..23825)
                     /gene="msrC"
                     /locus_tag="M2A35_003204"
     CDS             complement(23328..23825)
                     /gene="msrC"
                     /locus_tag="M2A35_003204"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCB7199464.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            23955..25238
                     /gene="yebS"
                     /locus_tag="M2A35_003205"
     CDS             23955..25238
                     /gene="yebS"
                     /locus_tag="M2A35_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCB7199465.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            25207..27840
                     /gene="yebT"
                     /locus_tag="M2A35_003206"
     CDS             25207..27840
                     /gene="yebT"
                     /locus_tag="M2A35_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCB7199466.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYISMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            27920..29359
                     /gene="rsmF"
                     /locus_tag="M2A35_003207"
     CDS             27920..29359
                     /gene="rsmF"
                     /locus_tag="M2A35_003207"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCB7199467.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRDLIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRSKNSYPRELVRDGKLFTGNA"
     gene            29477..29713
                     /locus_tag="M2A35_003208"
     CDS             29477..29713
                     /locus_tag="M2A35_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCB7199468.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            29818..30009
                     /locus_tag="M2A35_003209"
     CDS             29818..30009
                     /locus_tag="M2A35_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCB7199469.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(30010..30666)
                     /gene="pphA"
                     /locus_tag="M2A35_003210"
     CDS             complement(30010..30666)
                     /gene="pphA"
                     /locus_tag="M2A35_003210"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCB7199470.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(31131..31685)
                     /gene="pinE"
                     /locus_tag="M2A35_003211"
     CDS             complement(31131..31685)
                     /gene="pinE"
                     /locus_tag="M2A35_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HCB7199471.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGVSTLYKKFPVGDK"
BASE COUNT         7877 a         7914 c         7628 g         8331 t
ORIGIN      
        1 tctgacgaga agagccgcac ggatatggtg atcctgttgc aggtgaaaaa agtctgagtt
       61 ccgtttgagg ctgattattc gcactgccat catatgaaat gactgtgcga ataatttgat
      121 atgagttatg taaaaagcta ttgctatacc gtacaatgtc gcgctcttgt ctatagtttt
      181 atcacatatc tgttctcctt taagtctagc cacagttaca caaaaaaact tctatttttt
      241 tgctgtttta tttgatgttt ttttcgattt ttagtggcta gattatcaaa ggcttttatc
      301 taaagagtat ttacaatgga tttcttactt tttttattaa tctccctact ccccgcattc
      361 ttctttttga aaaacaaaaa aaaaacaaaa aagtacaaga ctcgagccat catatattgg
      421 cagaaaacaa ataaatcgac catcaaaaaa tggaaaaagt gataattacg acagttatct
      481 tttttataaa caaaaacaat tcatgacaaa tcgtgagcgt tatttcttca acttgctcta
      541 tggtcaattt agccataatt atttcttgtt ttcacaggtc agaatggtag atcttattac
      601 tcccgtattt gataaagata ctcagtataa ttcttttatt cgtgcattca gatcaatatc
      661 ccaatatcac attgattttt taattgttag tagaattgat ttttctattg tgtgcgcaat
      721 tgagcttgat gattcatcac atgatttagc ttcacgcgta aaacgagata ataagataaa
      781 tttaattttt aaattcatag gcattccatt atttagatca agaaaccctt tagcattatt
      841 ggaaatggta aaaaaacata tagattttca aaatgtaaat taactccatt gtttcaatca
      901 agcctgttca aaacttattc aattagcaac taacatagtt gctgttgatt tgaacaggga
      961 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg
     1021 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
     1081 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
     1141 gtgtaccgga aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga
     1201 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
     1261 tcctggctga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg
     1321 cgggtcatgc gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag
     1381 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
     1441 atttatcgat aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt
     1501 gatgctcctg ctaattcatt atttattttt ataatttcaa ttttagctat aaagttaact
     1561 atataaataa acataaccat gccgttgtgt tcgccctctg actagcaact ctggttttct
     1621 ttcttagctg aataatctgg ttatagcgtg aggactgacc gttaaatttc tccatcttac
     1681 tggataacac accatgagca gctaccttta aaacacctga atgtcaacag gttaactcgt
     1741 gccatatcgt tataatccct ttgatattag atgcaaatta aggttatata tagtcttatc
     1801 gataacatgg ttaattttaa ggataagaat atgcctgctg taatagataa agccctggat
     1861 ttcattggtg ccatggatgt atcagcgcca acaccaagtt cgatgaatga aagcacggcg
     1921 aagggaatct ttaaatattt aaaagaactg ggagtacccg cgagtgccgc tgatattacg
     1981 gcgcgagctg accaggaagg ctggaaccct gggttcacgg aaaaaatggt tggatgggca
     2041 aaaaaaatgg agacaggtga acgctctgtg attaaaaatc ctgaatactt ttcaacatat
     2101 atgcaggaag aactaaaagc actggtctga gttaaattta tatcagcata aatgggtcag
     2161 gacgctttta atcacatata aaaagcgtcc tttttctcac aatcaaatga tcatcggctt
     2221 ctataacgaa atcgatacgg attagtgtgt tactcgtctt catcctcaaa acgcgccacg
     2281 attcgctcgc cggagtggtt ggcacgcagc tcttctgcca ccagcgcgat tgcctggccg
     2341 ctgctcatcc cttgtgccat cagttcctgg atccgctcga cagctttttg ctgctgttca
     2401 tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct gctaaattgt tggcgctaat
     2461 tatttcatgc tacccggcac atagccagta gagtcaggac tgatgaagac gttatctccc
     2521 gctgtgatta ctttaccctg gcgtcaggac gccgctgaat tttatttctc ccgcttaagc
     2581 cacctgccgt gggcgatgct tttacactcc ggctatgccg atcatccgta tagccgcttt
     2641 gatattgtgg tcgccgatcc gatttgcact ttaaccactt tcggtaaaga aaccgttgtt
     2701 agtgaaagcg aaaaacgcac aacgaccact gatgacccgc tacaggtgct ccagcaggtg
     2761 ctggatcgcg cagacattcg cccaacgcat aacgaagatt tgccatttca gggcggcgca
     2821 ctggggttgt ttggctacga tctgggccgc cgttttgagt cactgccaga aattgcggaa
     2881 caagatatcg ttctgccgga tatggcagtg ggtatctacg attgggcgct cattgtcgac
     2941 caccagcgtc atacagtttc tttgctgagt cataatgatg tcaatgcccg tcgggcctgg
     3001 ctggaaagcc agcaattctc gccgcaggaa gatttcacgc tcacttccga ctggcaatcc
     3061 aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg tacaggaata tctgcacagc
     3121 ggtgattgct atcaggtgaa tctcgcccag cgttttcatg cgacctattc tggcgatgaa
     3181 tggcaggcat tccttcagct taatcaggcc aaccgcgcgc catttagcgc ttttttacgt
     3241 cttgaacagg gtgcaatttt aagcctttcg ccagagcggt ttattctttg tgataatagt
     3301 gaaatccaga cccgcccgat taaaggcacg ctaccacgcc tgcccgatcc tcaggaagat
     3361 agcaaacaag cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa tctgatgatt
     3421 gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag ccggttcggt aaaagtacca
     3481 gagctcttcg tggtggaacc cttccctgcc gtgcatcatc tggtcagcac tataacggcg
     3541 cgactaccag aacagttaca cgccagcgat ctgctgcgcg cagcttttcc tggtggctca
     3601 ataaccgggg ctccgaaagt acgggctatg gaaattatcg acgaactgga accgcagcga
     3661 cgtaatgcct ggtgcggcag cattggctat ttgagctttt gcggcaacat ggataccagc
     3721 attactatcc gcacgctgac tgccattaac ggacaaatat actgctctgc gggcggtgga
     3781 attgtcgccg atagccagga agaagcggaa tatcaggaaa cttttgataa agttaataag
     3841 atattacgcc aactggagaa gtaagacgtg gaataccgta gcctgacgct tgatgatttt
     3901 ttatctcgct ttcaactttt gcgcccgcaa attaaccggg aaaccctaaa tcatcgtcag
     3961 gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg ggttgttgct gactcagcgt
     4021 tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc ctggaggtgc agtcgatgac
     4081 acggacgcat cagttatcgc cgccgcgctg cgcgaagctg aagaagaggt cgctataccg
     4141 ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg atagcgtaac tggctatcag
     4201 gtaaccccag tggtcggcat tatcccgccc gatctgccgt atcgcgccag tgaagatgaa
     4261 gtctcggcgg tgtttgaaat gccgctcgcc caggcattac atctgggtcg ttatcaccct
     4321 ttagatatct accgccgtgg cgattcacat cgggtatggc tgtcctggta cgaacagtat
     4381 tttgtatggg gaatgaccgc aggcataatt cgtgagctgg cgctgcaaat tggtgtgaaa
     4441 ccctgactat acttatcttt acatctacaa aacactactt gagacaatca tcgcaatatt
     4501 agttaaatcg cggtttttga ttagtttaat tcatgtgaat agttaagcca gtcgccgcgt
     4561 tccctcttac actatgcgct gttattagtt cgttactgga agtccagtca ccttgtcagg
     4621 agtattatcg tgattagtct attcgacatg tttaaggtgg ggattggtcc ctcatcttcc
     4681 cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg atgatctggt cgaaaaaggc
     4741 ttactggata gcgttactcg cgttgccgtg gacgtttatg gttcactgtc gctgacgggt
     4801 aaaggccacc acaccgatat cgccattatt atgggtcttg caggtaacga acctgccacc
     4861 gtggatatcg acagtattcc cggttttatt cgcgacgtag aagagcgcga acgtctgctg
     4921 ctggcacagg gacggcatga agtggatttc ccgcgcgaca acgggatgcg ttttcataac
     4981 ggcaacctgc cgctgcatga aaacggtatg caaatccacg cctataacgg cgatgaagtc
     5041 gtctacagca aaacttatta ttccatcggc ggcggtttta tcgtcgatga agaacacttt
     5101 ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt tcaaatctgc caccgaactg
     5161 ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc tcgctatgca gaacgaactg
     5221 gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc atgtctggca aaccatgcag
     5281 gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc caggcccgct gcgcgtgcca
     5341 cgtcgtgcgt ctgccctgcg ccggatgtta gtttccagcg ataaactgtc taacgatccg
     5401 atgaatgtca ttgactgggt aaacatgttt gcgctggcag ttaacgaaga aaacgccgcc
     5461 ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg gtatcgttcc ggcagtgctg
     5521 gcttactatg accactttat tgaatcggtc agcccggaca tctatacccg ttactttatg
     5581 gcagcgggcg cgattggtgc attgtataaa atgaacgcct ctatttccgg tgcggaagtt
     5641 ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg ctgcgggtct tgcagaactg
     5701 ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa ttggcatgga acacaacctt
     5761 ggtttaacct gcgacccggt tgcagggcag gttcaggtgc cgtgcattga gcgtaatgcc
     5821 attgcctctg tgaaggcgat taacgccgca cggatggctc tgcgccgcac cagtgcaccg
     5881 cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa ccggtaagga catgaacgcc
     5941 aaataccgcg aaacctcacg cggtggtctg gcaatcaaag tccagtgtga ctaatacttc
     6001 ttactcgccc atctgcaacg gatgggcgaa tttatacccg ctttctcgtc tgctgtaata
     6061 ttccccacta cacttccact gttgcgtcag gcgtttgtcg ccatacgctt acagggtggc
     6121 ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc cgtaatcgaa tgattgtttg
     6181 tacgatttgc gcactcgtta cgctcgcttc gaccctgagc gtgcgattta tttcacagcg
     6241 taacttaaat caacaacggg tagtacaatt cgccaatcac gctgtagagg aattagataa
     6301 agtactgctt cccctacagg caggtagcga agtcttgctt ccgctgattg gtctgccctg
     6361 ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa ctccaaactg tgcgatccat
     6421 tggcctggtg caagacggca cactttattg ctccagcatt tttggttatc gcaatgtgcc
     6481 cgtcgtggac attctggctg aacttcctgc accgcaacca cttttacgcc tgacgatcga
     6541 ccgtgccctg attaaaggca gtccggtttt gattcaatgg acgcctgcag cgggcagtag
     6601 caatgctggg gtcatggaga tgattaacat cgacttactg acggcaatgc tccttgagcc
     6661 acaactgccg caaatcagta gcgccagcct gacggtggac aaacggcatt tgctctatgg
     6721 taatgggctg gtagattccc ttccgcaacc tgaagacaat gaaaactatc aggtttcttc
     6781 gcaacgcttt ccttttacca ttaacgttaa tggtccgggg gctatggcgc tggcatggca
     6841 ctatcttcca acacaattac cgctggcggt gctgctaagt ttactggtgg gctacattgc
     6901 ctggctggcg accgcttacc ggatgagctt ttcccgcgaa atcaatctgg gcctggcgca
     6961 acatgagttc gaattgttct gtcagccttt gcttaatgcg cgcagccagc aatgtattgg
     7021 tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc tggatttcac cggatgtgtt
     7081 tattcctatc gcggaagaac atcatttaat tgtgccactg acccgctatg tgatggcaga
     7141 aaccattcgt cagcgccatg ttttcccgat gagtagtcag tttcatgttg gcattaacgt
     7201 cgcacccagc cattttcgcc gtggtgtgct gataaaagat ctcaatcagt actggtttag
     7261 cgctcacccg attcagcaac tgatcctcga aatcaccgaa cgcgatgcct tactggatgt
     7321 tgattatcgg attgcccgcg agctacatcg taaaaacgtc aaactggcga ttgatgactt
     7381 cggcaccggc aacagttcat tttcctggct tgaaacatta cgtcctgacg tgctgaaaat
     7441 tgataagtca tttaccgcag ctataggttc tgacgcggtt aactcgacgg tgaccgatat
     7501 catcatcgcg ctggggcaaa gactgaatat tgaactggtg gcggagggcg tggaaacgca
     7561 agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt ttgcaagggt atttgtacgc
     7621 acagccgatg ccgctacgtg attttcccaa atggctggcg ggcagccaac cgccgcccgc
     7681 ccggcataat ggacatatca cgcccattat gccgttacgt taacgcttac tcatcttcat
     7741 cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac acgataatca ttggcttcaa
     7801 tgatggtgat atgcagtggc cctacatcaa tcacatcgcc cacacgggga atgtgaccat
     7861 ttgccgagat caccaggccc gcgaccgtcg cgatatcatc gtcatcggca aggtgctcaa
     7921 catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc ttttaccagc cagccgtcac
     7981 catcggtaat gatttccggc gtttcgtcag cgtccgggaa ttcacccgca atggcttcca
     8041 gcacatccag cggcgtgacc aagccttgta ccacaccaaa ctcgttggtc acgataacaa
     8101 agctcccgcg agcgcgacgc agcacgccca gcaggttgat cggatcgagg gtttccggga
     8161 cgataatcgc cggagacgcc gaagcaatcg ccgccacatc aacgccctct tccagcgcca
     8221 ccagcagttc tttagcacgt acgattccga tgatttcatc cagttcaccg cgacataccg
     8281 ggaacaggct gtgcggtgag gagagcagct gctcgcggat ttcatcgacc ccgagattag
     8341 cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc gcgcagagaa cgcgacgcca
     8401 gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc aaatgcacct tccgggatcg
     8461 gcatcggcat cgggttatcg gcatcgtgct gaacattggc ctgacgtttc ccgcccatca
     8521 aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa agtcgactgg tgacgaataa
     8581 agttgcgacg cgcaatctgg ttaaacactt cgatgatgat cgagaagcca atcgcggcat
     8641 acaggtaacc tttcggaatg tggaaaccga aaccttctgc caccagactc agaccaatca
     8701 ttaacaggaa gctcagacag agcaccacca ccgtggggtg ctggttaacg aatcgcgtca
     8761 gcggtttgga tgccagcaac ataaccgcca tcgcaatcac taccgccgcc atcatcaccg
     8821 gcagatggtt aaccatccct actgcagtaa ttaccgcatc caacgagaag acggcgtcaa
     8881 ggatgacaat ctgtgtgacg accacccaga aactggcgta gcctttaccg tggccggaat
     8941 catgatcgcg gttttccagc cgttcatgca gttcggttgt tgctttgaac agcaagaata
     9001 tccccccgaa caacataatc aggtcgcgtc cggagaagga gaaatccatg acggtaatta
     9061 gcggtttggt cagcgtgacc atccatgaaa tcagcgacag cagccccaga cgcataatca
     9121 gcgccagtga taaccccagc aaacgcgctt tatcgcgttg ttttggcggc agtttgtcag
     9181 caagaatggc gatgaagacc aggttatcga tacccagcac aatttcgaga acaacaagcg
     9241 tgagtagccc cgcccaaatt gaggggtcca ttaagaattc catgacaagc tcctgcttaa
     9301 ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg acaaaatgca aacgaaaggt
     9361 gcggcataga gagccagaaa ggcaggcgtt aaaaggcctg atgctgaaat gacgtcggtg
     9421 acgatccata ctgcgggcta ctgccctata ctccatggtt gttaaacggg agttaaacat
     9481 atcagagacg cctctgattt ggcaaagatt taccttcctt tgcaaacgaa tgtgacaagg
     9541 atattttacc tttcgaaatt tctgctaatc gaaagttaaa ttacggatct tcatcacata
     9601 aaataatttt ttcgatatct aaaataaatc gcgaaacgca ggggtttttg gttgtagccc
     9661 ttatctgaat cgattcgatt gtggacgacg attcaaaaat acatctggca cgttgaggtg
     9721 ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca cacatggttg
     9781 ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg aaaacgtcgg
     9841 ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt acaacgctca
     9901 gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat ggggaggcag
     9961 cccgttcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag tcattgcagg
    10021 cgttaacatt ccaatgctcg tggaaacgtt aatggcccgt gatgatgacc caagctttga
    10081 tgaactggtg gcactggcag tagaaacagg ccgtgaaggc gtgaaagctc tgaaagccaa
    10141 accggttgaa aaagccgcgc cagcacccgc tgccgcagca ccaaaagcgg ctccaactcc
    10201 ggcaaaacca atggggccaa acgactacat ggttattggc cttgcgcgta tcgacgaccg
    10261 tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct cccgtattat
    10321 tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag acactgctca cccaggttgc
    10381 acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg tctacaacaa
    10441 cccgaaatat gctggcgaac gcgtaatgct gttatttacc aacccaacag atgtagagcg
    10501 tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg cattccgtca
    10561 gggtaaaacc caagtgaata acgcggtttc ggttgatgaa aaagatatcg aggcgttcaa
    10621 gaaactgaat gcgcgcggta ttgagctgga agtccgtaag gtttccaccg atccgaaact
    10681 gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga ttatcactca
    10741 gttttcacac ttaagtctta cgtaaacagg agaagtacaa tggagattac cactcttcaa
    10801 attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct cgatgaattt
    10861 cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg ggatatgaaa
    10921 accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat gaacatcggt
    10981 gctgcagttg cgcctgacgc cgctctggct tctatcattt ctaccattct ggttatcgca
    11041 ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc ctctggccgc tgcgggccag
    11101 gtactgacca tcatcgttcg tactattacc gttgctttcc agcacgctgc ggataaggct
    11161 gctgataacg gcaacctgac agcgatttcc tggatccacg tttcttctct gttcctgcaa
    11221 gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgttggtac cagcgaagta
    11281 cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat cgccggtggc
    11341 atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg ctacctgatg
    11401 ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct ggttgctctg
    11461 ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa atacaaccgc
    11521 gtagccggtg cgcctgctca ggcagctggt aacaacgatc tcgataacga actggactaa
    11581 caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact cactcaaagt
    11641 gatattcgtg gcgtcttcct gcgttctaac ctcttccagg gttcatggaa cttcgaacgt
    11701 atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct ctaccctgag
    11761 aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg agttctttaa cacccagccg
    11821 ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg aagaacagcg tgctaatggc
    11881 gcagagatcg acgacggtgc tatcaacggt atcaaagtcg gtttgatggg gccgctggct
    11941 ggtgtaggcg acccgatctt ctggggaacc gtacgtcctg tatttgcagc gctgggtgct
    12001 ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct gtttaacctg
    12061 gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg tatcgatatc
    12121 gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc tatcctcggc
    12181 ctgtttgtca tgggggcatt ggttaacaag tggacacatg tcaacatccc gctggttgtc
    12241 tctcgcatta ctgaccagac gggcaaagaa cacgttacta ctgtccagac tattctggac
    12301 cagttaatgc caggcctggt accactgctg ctgacctttg cttgtatgtg gctactgcgc
    12361 aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat cgctggttac
    12421 gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt tggccccgtt
    12481 tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt catcgccgca
    12541 ctcctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg ccccaccctg
    12601 ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt cggattgatt
    12661 gtcattctta tctataacaa cgtcacgaat catggtgcgt taataacgac atggttatta
    12721 agcgcactgg ctctgatggg tttttatata ttctggatcc gcgttccgaa gatcatcttt
    12781 aaacaaaaag gttttttctt cgccaatgtc tggattgaat atagccgaat caaagcgatg
    12841 aacttgtcgg aagatggcgt gttggtgatg caattagaac agcgtcggct gttaatccgc
    12901 gttcgaaata tcgacgatct ggaaaaaatt tataagcttc tcgtttcaac tcaataagtt
    12961 atgaatttag ccaaagctat gtttagtgta tttttaataa tcagacatag cttaggctat
    13021 attacctctt cccttatttg ttatttattt taacgtttca ttgatatata aatctaaatg
    13081 taaatcgtta tcaataaagc aatgaaataa tatattccaa cagttgtttt atattctcaa
    13141 aatatgttaa ggttgcgccc tcatttgggg agtagccgat ttccagattc cggaaatgta
    13201 cgtgtcaaca tactcgttgc aaaacgtggc acgtacggac tgaatacttt cagtcaggcg
    13261 agaccatatg cacatcaatc gctatgcctg catgaacgcg aaaaccatcg cggcttgttg
    13321 ttcatgtatg catgtttgct gggggcgatg atgtgcttta tggatacccc ggtcaggaca
    13381 ttgtcatgaa tatcactgct actgttcttc ttgcgtttgg tatgtcgatg gatgcatttg
    13441 ctgcatcaat cggtaaaggt gccaccctcc ataaaccgaa attttctgaa gcattgcgaa
    13501 ccggccttat ttttggtgcc gtcgaaaccc tgacgccgct gatcggctgg ggaatgggca
    13561 tgttagccag ccggtttgtc cttgaatgga accactggat tgcgtttgtg ctgctgatat
    13621 tcctcggcgg gcgaatgatt attgagggtt ttcgtggcgc agatgatgaa gatgaagagc
    13681 cgcgccgtcg gcacggtttc tggctactgg taaccaccgc gattgccacc agcctggatg
    13741 ccatggctgt gggtgttggt cttgctttcc tgcaggtcaa cattatcgcg accgcattgg
    13801 ccattggttg tgcaaccttg attatgtcga cattagggat gatggttggt cgctttatcg
    13861 gctcaattat tgggaaaaaa gcggaaattc tcggcgggct ggtgctgatc ggcatcggcg
    13921 tccagatcct ctggacgcac ttccacggtt aataagaacg ctgccagagg tgaatattaa
    13981 agtccgtctg gcagtcgaac acttcttttg ctgccagtgt ttgccagact tctggcttcg
    14041 cacgccaggc aaacggcgtc atctgcaata atgcgacggc ttcatcaccg cgaagacgca
    14101 tcggataaca caactcatca ctctgctgta atgtaaaacc ttccagttgt tctgcatgag
    14161 gtgcatgaag atgtacttca ttgtaaatca gccccttcag ctccatcaaa tgtcgcggtc
    14221 ccggcgtggc agtaatgacc cagccgccgg gcttcactac tcgtgctaat tcttctgctt
    14281 tacacggcgc gtaaatacgt attatggcgt ccatactggt atcggaaaac ggcaaacggt
    14341 ggctggaagc gacacaaaga gtgacctgcg gatagcgttt cgccgccgct tttatcgcta
    14401 ccttcgagac atccaggcca aacgtggtga tttcgggcaa cacatcggca aatgcgtgtg
    14461 tgtaataccc ttcaccacag ccaatatcca gcaccgccgt ggccttttca tcaagccgtt
    14521 cccgcagttg ggcgacaatt gcatcacgca gcggctgata atgtccggca tctaagaatg
    14581 cgcggcgtgc ttgtatcatt tccgcgctgt cgcccggatc acgagaccgt ttatgctgaa
    14641 cgggcagcag attgacatac ccttctttcg ccatatcaaa ctgatgtcgc tggggacaga
    14701 tatagctgtt tttttcacgc gaaagaggct gatggcaaag tggacaggaa aacgacatga
    14761 caactccggc aggatactaa aggccgcaag tgtaacgcga atcacgcggc gggcaaatat
    14821 ccgaacgcca ggagcgaaga taaagcatag taaaaagcct cgcattcgac gaggctttat
    14881 agcgagattg aagcgtattc acacttcaga tcagtggatt cgatcagata gctgttacgt
    14941 taacagctgc cggacctttc tggccgtcct gaatttcgaa ctcaacgttc tgaccttcag
    15001 ccagagtttt gaagccatta ccctggatag cggagaagtg tacgaacaca tctttgctgc
    15061 catcagccgg agtaatgaag ccaaaacctt tagactcgtt gaaccactta acctgacctt
    15121 taatctttgc catttgaaaa attccttaga ttgttttctt cgcccgcagg cataacatag
    15181 ataaaactga cacattactg catgaggcac caatataagg ctcggcagag aagcggtatt
    15241 caacgtcaac gtgtttactc aggacttctt tactgaaaat gccacacata aacagaactg
    15301 tacctcgttt aacccgaaat ctgttatcac atacaacgtt aattatggca agccattttt
    15361 aaacatgtct cgatcagacg cacaaatctc gatacgtctt ccactttttt tgcacactta
    15421 tgcaacggaa tacgcgccga tatatcattg tgcttaacct tgccagttca ggcagatact
    15481 taaactggcg tattttctaa catagttcaa taagttcagt tgttccaggc cagtgggaga
    15541 agttattaca tagtgcgtgc aatatcacat tttttgctat gcaatgaata aaaagttata
    15601 tcactttttc tcataaaaca gtcagttaac ggctatcaat taccctaaag agaagtcaat
    15661 ccccaaaggg attgaaaatt taaaataaga aaaattgatg aatgagcaaa aaaatcaaga
    15721 gagaaacgtt tcgctaataa ataataccac gcttaatcac aaatccgatg taaacatcct
    15781 aataaattaa tgaggaaaat atctttttct gatatcccgt tacgcaaata aataatatga
    15841 taattatttg aagtcagacc agagttttaa tttgaagcag ttgcaccatg gcagtgcgta
    15901 taatcaggat gaattgagta acccttcaac gacggggtta ctcaagattg aggaaggatt
    15961 atcgttcggc gaccagacga ataatatctt cgtcttcaac cgatttttga ctctcaacgg
    16021 tctttttatc tgctttctca ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt
    16081 gacgtttttg ctgatccagc aacgcctcaa gcagttcaat ctgttcgtta gtccgagaac
    16141 tggcacgatt aataaaaaac cacaagatga gcccgataag aagaaccacc accgatacaa
    16201 ccaaagacgc aatgttcatc acgcctgaat ttacaacttc gttcatttca ccacctctga
    16261 gtagaggcgc tattctacca ctgctggaga ggaagaaaat ctagtgctga aaaaatgata
    16321 tcaccacggg ataaactggt taatggcaca aattccgctg aaaaattgta catcctgatc
    16381 gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa gccaacacca cgacaaccag
    16441 aacgacccat cgaaactttt tcactccaat ctccgtttca cctaccttat gtcattaaac
    16501 tagcatgcgt tagttaaaca gcgcctaact atgtcgatat tcgtgctttt tcggaatgag
    16561 tcgcttgctt cagcgcatat tgccgctacg attaagcgaa cataaaaaga gaagaggttg
    16621 taatgcgatt catcattcgc acagttatgc tgatagcact ggtatggata ggattattac
    16681 tcagcggata tggcgttttg attggaagca aagagaatgc cgcaggatta ggtttgcaat
    16741 gtacctatct gactgccaga ggcaccagca cggtacaata cttgcacact aaaagtggtt
    16801 tcttggggat aacagattgc ccactgttac gtaaaagcaa tatcgtggtc gataatggtt
    16861 aagggtaata aataaccaga ttctcactca tgagtaaaat attcttatag caagcgcgtt
    16921 taatataacg caccttcaac tttatttttc tcatgctaaa taaagaacct gtctgtttga
    16981 tattccgaca ggttcttact ctcatcagaa cggatagtcg tgataaccca tttgggcaga
    17041 aattttgcgc gctgcggtat gcaacattgc gacatattcc tgtaaacgct cttcagagaa
    17101 acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac
    17161 cggtaccgca atgcatcgca gcccttcttc ctgctcttcg ttatcttcgc catacccctg
    17221 ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta ctggtgatgg tccgctcggt
    17281 actgcgttta tactccacgc cctcaagaat ttgcttcact tcatcgagat cgcgccatgc
    17341 cagcagtacc ttaccaatcg cggtgctgta cagcggatta cgacgcccaa tccgtgaata
    17401 catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa acaatactgt cttcgtccag
    17461 tgcgccgagg tggatagttt ctttggtcag gcgggagatc tcacgcatct ggatatctgc
    17521 gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc agttcaaaca atttcagggt
    17581 cagggaatat ttctccgact ccccttcctg cgccacataa cctaaggttt tcatggtctg
    17641 taaaaagcga taaacggtgc tttttgacat catgacgcgc agcgacagct cggttatccc
    17701 tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa acttttagca cggaagatac
    17761 agaatcaggc tgtttatcca gatctgcgtt agccatttat cacctcattg cgagtgtttt
    17821 ataaaaatca gaactgtttt ttattataat ttcgcaccaa ggtggtcgca atccatcttt
    17881 tgccggttag ttacaattct gcgacatcca ccgtgaatat cagtgctaga atcatacccc
    17941 tgttgattat tcaccaaaga tataaaattc ctatgccaaa agttcaggcc gacggcctgc
    18001 cattgcccca gcgatacggt gcgatattaa ccattgtgat tggcatttcg atggctgtcc
    18061 ttgacggcgc aatcgccaac gtcgccctgc caacaatcgc cacggacctt catgccacgc
    18121 cagccagttc catctgggta gtgaacgcct atcaaatcgc cattgtcatc tccctgctct
    18181 cgttttcgtt tctgggcgat atgtttggct atcgacgtat ttataaatgc ggtctggtcg
    18241 tttttctgtt gtcttcactg ttctgcgccc tttctgattc gctgcaaatg ctcacccttg
    18301 cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag cgttaatacc gcacttatcc
    18361 gcctgatcta tccacaacgt tttctgggga gagggatggg cataaactcg tttattgttg
    18421 ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat cctctccatc gcatcctgga
    18481 aatggttatt tttaatcaac gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt
    18541 ttctgccacc caatggttct cgcgccagta aaccccgttt cgacctgccc agcgccgtga
    18601 tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag tggtttcgct caggggcaat
    18661 cgctgacgtt aattgctgcg gaactggtgg taatggttgt tgttggtatt ttctttattc
    18721 gccgccagct ttctcttccc gtaccgctgc taccggtgga tttactgcgt atcccgctgt
    18781 tttcactttc tatttgcaca tctgtttgct ctttctgcgc acaaatgctg gcaatggttt
    18841 ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc
    18901 tgacaccgtg gccgttagca acgatggtga tggctccgct ggcaggctat ttgattgaac
    18961 gcgtacatgc aggattgctg ggtgctttag ggttgttcat catggctgcg gggctttttt
    19021 ccctggttct gctgcccgcg tcacctgcgg atatcaatat tatctggccg atgatcttat
    19081 gtggcgctgg atttggctta ttccagtcac ccaataacca caccattatt acctccgctc
    19141 cgcgcgaacg tagcggtggg gccagtggca tgttaggaac ggctcgtcta ctgggtcaga
    19201 gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt cggtgataat ggtacgcacg
    19261 tttcgctgat ggctgcggct attctggcag tgattgctgc ctgtgtgagt ggtttacgta
    19321 tcactcagcc acgatccagg gcataaaaaa agcgcgtcga tcaggacgcg ctttttagta
    19381 tttacttcat attacttcag gtattcaccc gtacgcagag cttcaattcg tttatccagc
    19441 ggcgggtgag tcatgaacaa ctcactgagc gatttcgact taccgttaat gcagaaagcc
    19501 atcatgctgg ttgcttcttg cggttcatag ctggttttca ggcgctgcag cgcggcaatc
    19561 attttctcgc gaccaaccag ttttgccgaa ccggcatcag catggaattc acgatgacgc
    19621 gagaaccaca tggtgataat gctcgcaaga ataccaaaca ccagttccag aaccgttgca
    19681 accgcaaagt agatcagcgg gttgccgttg ctctcttcac cttcatcacg atttccgccc
    19741 ataaaacccg cggcaagctg cgccagaata cgggaaataa agataacgaa ggtgttcacc
    19801 acgccctgaa tcagcgtcat ggtgaccata tcaccattgg cgatgtggct gatttcgtga
    19861 gcaattaccg cctcggcttc atccgggctc atgttctgca gcaaaccggt gctgacagca
    19921 accagagagg catcacggcg cgcaccggtt gcaaaagcgt tgatgtccgg cgcatggtag
    19981 atagccactt gcggcatagc gatccctgcc tgacgagcct gggttgctac agtattaacc
    20041 agccaacgtt ccctttcgtt acgcggttgc tcgatcactt ccccgccaac agatcgtaat
    20101 gccatccatt tggacatcag aagcgaaacg aaggaaccac caaaaccgaa cagcaaggcc
    20161 atgatcatca gcccctgaac gctgctcgac tgtatccctg tcaggctcag taccagcccg
    20221 aaaacgacca ttacggccag gttcgttagc aggaagagcg cgattcgcat cataattttc
    20281 tttttacctc agtttaacaa aacgcaatat gcgataccca catcgtatgg gttacgcgac
    20341 tattttcaag tctggatagt gcgtaagtca ccagaaagac acaactttac attttgtagc
    20401 atctgattta cggcatcttg ccgctgttaa aaaatcaggc acaatttctt gtgcctgatt
    20461 gatattactt gacgggagcg ggttgttccg cgggtctggc tttttcaagc ttcgccagat
    20521 cgagtgcgat attcaccgtc tcatccagat aaggatccgg ctcctggtaa tctttaggta
    20581 gatcatccag cttcttcaac tccggtttac cttcgcgttt aaagcgttcg ttcaaacgcg
    20641 ccagacgcgt cgcatcatct tcattattct ctttctcacg cacagcgtag ttcagagaaa
    20701 cgatattgcg cttgtccttc atagcgttga agcgcgcgat atccttcatg atgttctgga
    20761 actcaggatc tttcgcgata cgcgcattat gttccttcag cagctccggc ccaaaggccg
    20821 ttaaatcgcc tgatttcaca taagtcgcgg catcaatgct atcccacggc agcgcgttat
    20881 cttcgaattt ctcacccgtt tccgtttctt cattacccgt cggcatgatg atgtctggcg
    20941 ttacgccttt acgttgcgta ctgccgccgt taacgcgata gaatttctgg atcgtgtact
    21001 gcacagaacc cagcgctggc cattcaggac gtaacatctg atcgtaaata cggttcaatg
    21061 aacggtattg ctgaacggtg cctttaccaa acgtcggttc acccacaacc agcgcacgac
    21121 cgtaatcctg cattgccgcg gcaaagattt ctgaagccga agcactgaaa cggtcaacca
    21181 gtaccaccag cgggccttta tagaacacct gcccgtcggt atcgctgtct tcacgaacct
    21241 tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg aataaacaga cctgagagcg
    21301 ataccgcttc ggtcagcgcc ccaccgccat tgctacgcag gtcgatgatc acactgctga
    21361 cattctgttt ttccagtttc tgcagttgaa ctttgacatc gtctgtcaaa cccacataga
    21421 agcccggaat atccagcacg ccgactttct ctttaccgac ggtcttcacc gacattttaa
    21481 ccgcgcggtc ttcgagacga atacgttcac gggtcaatgt tacagtgcgg gtcttggtcc
    21541 ctttaccagc aggtaaaatt tccagacgaa ctttactgcc cttcggccct ttaattaagg
    21601 caaccacatc atcaagacgc cagccaatca cgtcaaccat cggcttgcct gtttgaccaa
    21661 caccgacaat tttgtcacca acgctgatag ctttactctt cgctgccgga ccacctgcca
    21721 ccatcgaatt gataacggtg tagtcatcat ccatttgcag cactgcgcca ataccttcca
    21781 gcgacaaact catttcagtg ttgaactgtt cggtattacg cggggaaaga tagttggtat
    21841 gcgggtcgat ttcacgcgca aacgccgtca ttgccagcga gaaaacatct tcgctgttgg
    21901 tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt cagggtttca cgaatttctt
    21961 tatccgtttt tcctgccagc ttcaggctta actcgtcgaa tttgacttta ctgtcccaca
    22021 gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt gctgcggtca aggttataag
    22081 tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga caaagcgtac tggtaacgct
    22141 caaaacggcg cttttgcgcc agattgtaga gatcgtagaa aacgtcgagt ttgcctgaac
    22201 gcagttcatc gcctaactcg gttttctttt tcgcgaactg ttcaacatcg cttgccagca
    22261 gcacgttgtg gctgtaatcg agcagattca ggtagcggtc aaagattttg gccgaaaatg
    22321 cctgatcgag gtcgaactgg cgataatgag aacgggtgaa gcgcgacgtt acgcgctcac
    22381 ttaccgtcgc atgctgcgtc tcttccttta ataccggaat ttgatcagca cgcgtgatat
    22441 cttctacagc gaaggtctgg cctgctattg caagcaggcc agctaacgcg gtaagcctaa
    22501 aaaacatgtt catgcctggc ccggcctccg tttcagaaca ccaggtgttc tgcgcgcaca
    22561 atcaaagaca tacccgaatt cagctggacg cggacgccgt ctttggtgat ttctaatacg
    22621 gtggcatcca tcgcgttttg acccgctttc accttcaggg cttgtccgac agtcagagct
    22681 gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg cttttactgt ttttggcgct
    22741 ttctctaccg gcttttgcgc acgaggttta cgttcagcgc cttctttgcg gcgtggcgta
    22801 gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt tctcaccagc agctgcggca
    22861 gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct gaacacgcgc tttcgcttct
    22921 tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca gctcaccgca tgggttgccg
    22981 tcaagatcga cacgcgttgc gccgggttta acaccgtaaa gataacgcca gctcgaagtg
    23041 tagagacgta aagcggatcg taattgcgtt ttgctcaggt tcatttcccc ggcaacacga
    23101 tcgaccaaat cctgaaaaat accgattttc agcggacgcg cttcaccttc cgcactgaaa
    23161 cagtggggaa aacgttcggc cagaaacgcg attacttctt tactgctatt caacttaggt
    23221 tgattttcca tgaaatttcc tgattacaac ggacgtagcc aacaagcgca ggcatgaaca
    23281 ggcgtcatta taatgacgct accagtaaat gctacgttat ccgttgatta tcctgcgacg
    23341 ctcgcaaaga attttttgta atccgtcgtt gcaagcactt tttcaagctg tgccacaagc
    23401 tgacgtaagc cttgctcgtc ctcgtctgta aagcgaccga agacggtact atcgatgtca
    23461 agaacaccaa taatctgatt tttcaccacc agcggcagaa caatttcaga attactcgcc
    23521 gcatcacagg caatatgccc gtcaaacgcg tgaacatcct cgatacgctg cacttgattg
    23581 cgggcaaccg cagtgccgca caccccgcgc ccgacgggta tccggacaca ggcaattttg
    23641 ccctgaaatg gtcctagtac cagtgtatcg tcctcaagca aataaaaacc tgcccagttt
    23701 acgtcagtga gacgctcgta taacaacgca ctggtgttcg caagcgttgc cagaaaactg
    23761 gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat ccgcgtaaaa ttctgttttg
    23821 ttcattatat aatcacttgg ttgtcttacc tggaactgcc agcctattaa aataagcatt
    23881 aaatgcgtta atgctcaaga tcattccaat catgggttaa tattaatgtt aattcttatt
    23941 acatttggca cgtcatggct cttaacacac cacaaattac gccgacaaaa aagataacag
    24001 tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg caatgtgaca
    24061 tgctgtttag cctgcccgag ataaattctc atcagagtgc ctattgtccg cgctgtcagg
    24121 caaaaattcg cgacgggcgc gactggtcgc taacgcgcct ggcggcaatg gctttcacca
    24181 tgctgttgct gatgccgttt gcctggggcg aaccgctgtt gcatatctgg ctgttaggta
    24241 ttcgtatcga cgccaacgtc atgcaaggca tctggcaaat gaccaaacag ggcgatacga
    24301 taacgggggc gatggtcttt ttctgcgtta ttggtgcccc cctcattctg gtgtcctcca
    24361 tagcatattt atggtttggt aaccgactgg gaatgaattt acgtccggta ctgctgatgc
    24421 ttgagcgact taaagagtgg gtaatgctgg atatctacct ggtcggcatt ggcgttgctt
    24481 ctataaaggt acaggattat gcccatatcc aggcgggtgt cggcttgttc tcttttgtgg
    24541 cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaggag ctgtgggagc
    24601 gattttatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc tgtcttgggt
    24661 gccattttac cggctatcct gatcagcgtg gtcgctgccc gcgttgccat atcccgctac
    24721 gcctgcgtcg ccgtcatagt ttgcaaaaat gctgggcggc gctgttagcg tcaatcgttt
    24781 tgttgttacc tgccaacctg ttgcctattt ctatcattta tctgaacgga ggacggcagg
    24841 aagatacaat tctttccgga attatgtcgc tggcaagtag caacattgcg gttgcgggaa
    24901 tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg ttcactttac
    24961 tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg ttactgcgga
    25021 tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct ttaaccatgt
    25081 cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg ttttatttcg
    25141 gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc ttactttggg
    25201 atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa aaataaacgc
    25261 cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgccgg ttggctgatt
    25321 tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc ggcggatggt
    25381 attgtcccgg gccgtacgcc tgttcgttat cagggcgttg aagtcggaac agtgcaggat
    25441 atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa gtccgatatg
    25501 aaagatgcgc tgcgcgaaga gactcagttc tggctggtga cgccaaaagc atcgttggca
    25561 ggtgtctccg ggctggacgc cctcgtcggt ggtaactata tcagcatgat gccaggtaaa
    25621 ggtaaagagc aggatcactt tgtcgcactc gatacccaac cgaaatatcg actggacaat
    25681 ggcgatctga tgatccacct gcaagccccc gatctcggtt cgctgaacag cggttcattg
    25741 gtctatttcc gcaagatccc tgtgggaaaa gtctacgact atgccatcaa tcccaacaag
    25801 caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt gaaaaaaggt
    25861 agccgtttct ggaacgtttc cggcgttgat gccaatgtca gtatcagtgg cgcgaaggtg
    25921 aaactggaaa gtctggcggc actggttaac ggtgcgattg ccttcgattc acctgaagag
    25981 tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc ccacagccag
    26041 cgtggcgtaa taataaaact ggaactgccg agtggggcag gattaaccgc cgactcgacg
    26101 ccattaatgt atcagggact ggaagtcgga cagctaacta aactggattt aaatcctggt
    26161 ggtaaagtca ctggggaaat gacagttgat cccagcgtcg ttaccctgct tcgtgaaaat
    26221 acccgcatcg aattacgtaa tccgaaatta tcccttagcg atgccaatct cagcgccctg
    26281 ctgactggca aaaccttcga gttggtaccc ggcgatggcg agccacgcaa agagttcgtt
    26341 gttgtgccag gcgaaaaagc actgctgcat gaacctgatg ttctgacgct gaccctgacc
    26401 gcgccggaaa gttacggtat tgatgctggt cagccgctca ttcttcacgg cgtgcaggta
    26461 ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt cgccatcgag
    26521 cctcagcatc gagaactggt aaaaggcgat agcaaatttg tcgtcaacag ccgtgtcgac
    26581 gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga atggattaac
    26641 ggcgggatac gtattctgcc gggcgataaa ggcgagatga aagccagcta tccactgtat
    26701 gccaatctgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac aaccgtgagt
    26761 ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctcta ccgtaaattt
    26821 gaagttggtg aagttattac cgtgcgtccg cgagctaacg cgtttgatat cgatctgcat
    26881 attaagccgg agtatcgcaa ccttctgacc agcaatagcg tgttctgggc agaaggcggg
    26941 gcgaaagttc agctgaatgg tagtggcctg accgtacagg catccccgct ctccagagca
    27001 ttaaaagggg ctattagctt cgataacctc agtggtgcca gcgccagtca gcgtaaaggc
    27061 gacaaacgta ttctgtatgc ttccgaaaca gcggcccgtg cggttggtgg gcagattacg
    27121 cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta tctcggtatt
    27181 gatatcgggc aaatccagac gctggatctg attaccgcgc gcaatgaagt acaggcaaag
    27241 gcggtgctct atccggaata tgtccagacc tttgctcgcg gtggtacgcg cttctcagtg
    27301 gtcacaccgc aaatttcggc agctggcgtt gagcatcttg atactatcct ccagccgtat
    27361 atcaacgtcg aaccaggccg gggcaatcct cgccgcgact ttgaattaca agaggccacc
    27421 attactgatt cgcgttacct ggatggctta agcattattg ttgaagcgcc ggaagccggt
    27481 tcgttaggta ttggtacgcc tgtgctgttc cgtggtctgg aagtcggtac ggttacagga
    27541 atgacgctgg ggacattgtc agatcgcgtg atgattgcga tgcgcatcag taaacgctat
    27601 caacacctgg tgcgtaacaa ttccgtcttc tggttggcat cgggttacag tctggacttt
    27661 ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc agtttatccg tggcggcatc
    27721 gccttcgcca cgcctccggg tacgccactg gcaccgaaag cccaggaagg caaacacttc
    27781 ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg gaactgcgct tcccaaataa
    27841 tgcccactgc tccggcgtgc ctgcgccgga gcgtttatgc taaactgcgc gcctgttttt
    27901 ttgccagtgg tacatgctcg tggcccaaca caccgtttat ttcccggacg cctttctgac
    27961 acaaatgcgc gaagcgatgc cttcgacgct ctcatttgat gattttcttg ccgcctgtca
    28021 gcgcccgttg cgccgcagca ttcgcgttaa tacgctgaaa atctccgttg ctgatttcct
    28081 gcaattaacc gctccttatg gctggacgct tacgccaatt ccgtggtgtg aagaaggttt
    28141 ctggattgaa cgcgacaatg aagatgcatt gccattgggt agtaccgccg agcatttaag
    28201 cggcctgttt tatattcagg aagccagttc aatgttgccc gttgccgcct tgtttgctga
    28261 cgataatgca ccacagcggg tgatggatgt cgcagctgcg ccaggctcca aaacgacgca
    28321 aattgccgcg cggatgaata acgaaggggc aatccttgcc aatgagtttt ccgccagtcg
    28381 ggtaaaagtg ttacatgcca atatcagccg ctgtggcatc agtaatgttg cgctcacaca
    28441 ttttgatggc cgcgtgtttg gtgcggcagt gccagaaatg ttcgatgcca ttttgctgga
    28501 cgctccctgc tctggcgaag gcgtggtgcg taaagatccc gatgcgctaa aaaactggtc
    28561 accagaaagc aatcaggaaa tcgcagctac acaacgggat cttatcgaca gcgcctttca
    28621 tgcattacgt cctggtggta cgctggttta ctcgacctgt accttaaacc aggaagaaaa
    28681 cgaagccgtt tgcctgtggc tgaaagagac ttaccccgac gcagtagagt ttttaccact
    28741 tggcgatctc ttccctggtg caaacaaagc gctgaccgaa gaaggctttt tgcatgtttt
    28801 cccacaaatt tacgactgcg aaggcttctt cgttgctcgt ctgcgtaaaa ctcaggcgat
    28861 ccccgcctta cccgccccca aatacaaagt cggtaatttc ccgttcagcc cggtgaaaga
    28921 tcgcgaagcc ggacaaattc gtcaggcggc tgcaagtgtt ggcttaaact gggatggaaa
    28981 cctgcgactc tggcaacgcg acaaagaact gtggttgttc ccggtgggca ttgaagccct
    29041 gatcggtaaa gtccgatttt ctcgcttggg gattaaactt gccgaaacgc acaacaaagg
    29101 ttatcgctgg cagcatgaag cagttattgc ccttgccacc cccgacaatg tgaacgcttt
    29161 tgaactgaca ccgcaggaag cggaggagtg gtatcgcggg cgcgatgttt acccgcaagc
    29221 cgcgccagtg gcggatgacg tgttggttac tttccagcat caaccgattg gtttagccaa
    29281 acggattggt tcgcgatcga aaaacagcta tccgcgtgaa ctggtgcgcg atgggaaact
    29341 ttttaccggt aacgcctgac agcgcacaaa aaaagcgcac tttttgactg gcacattcgg
    29401 ctgcctcaac taggctgaaa aatggtgcga tcggactggt cgtaccacaa ccggcagcta
    29461 aatggagagc acgaagatga aaaccagtgt gcgcataggc gcttttgaaa tcgacgacgg
    29521 cgaattacac ggtgaatcgc cgggagatcg aacgttaacc attccttgta aatctgaccc
    29581 cgatttatgt atgcaactcg atgcctggga tgctgaaacc agtatccctg ccctgctaaa
    29641 tggcgaacac tctgtccttt accgtacccg ttacgatcaa caatctgatg cctggattat
    29701 gcgtcttgcc tgatccaaaa agaacccgtc ggcatggcgg gttatttgtc ctggttattc
    29761 ccccgttgta aaatctctcc taaacttaac ggtacggcac cacacttcgg ggatgaaatg
    29821 ttcgcgctgg tactttttgt ttgctacctg gatggcggtt gtgaagatat tgttgtggat
    29881 gtctacaaca cggaacagca gtgtctttat tctatgagcg atcaacggat ccgccatggc
    29941 ggttgttttc cgattgagga ttttatagat ggtttctggc gaccagcaca ggagtatggt
    30001 gatttttaat tattgcaatt gcacaagagt cagttcgccc ccaaagacag caccggtatc
    30061 aatataatgc aggttgccaa tatccacgcg atgtcgcaac ggtgtatgac caaaccagaa
    30121 atgatcagca cctgtaattc cctgcccttt ttggcgttca cctaatcgcg agcggctcca
    30181 caagacctga tgcaaatcaa cgtccttttg ccattcataa acatcatctg gataatcggc
    30241 atgagcaata acatgtttgc cggtacgact gtgtacttca agaataaagg gcaaatgctg
    30301 acatttttcc agcgccgttt tcgcttgttt ctgttgatta tctgccagcg caataaacca
    30361 gtcgccgcca ttcatcaacc acaaagacat ctgctgggat gccagcgcat ccatcgccat
    30421 ctgttcatga ttgcctctta ccgcacgaac ccaatgttgt tccagtaact gcaggcaacg
    30481 taaactttgc ggcccacgat cgataacgtc tcccactgag ataagtaaat ctcgccacgg
    30541 atcaaaacca caatgccata atttgcggcg caactgctca agacaaccgt gtatatcgcc
    30601 agaaagccag atatgtcgcc attgatgacc cgcaattctc tgataaacgg gcgcaggctg
    30661 tttcatcaat attttcctcc cgcgctaaag atcacataat cttaacaaga atgttaaaaa
    30721 acgctggact cagacagtag agtgtgtgtt atggttgact ataaagtcag cgaaggaaat
    30781 gcttctggct tttaacagat aaaaagagac cgaacacgat tcctgttttc gtcaacaaac
    30841 aagaaaccct ttttaaatta atgtgttaag caaaattacg ctcatctttt aatcccaaac
    30901 acgtaccatt gcatattaat gcattcaatt agttaccatt tttttcgagt tttttagaga
    30961 aaatttcggg actatttcag accaatccag gcagaaataa gcaatcccta tattgaatga
    31021 ttctgcaaca attatgtaaa atcacctcag gatgattttc actcaaactc gcgctatcga
    31081 acgtccatca gccagccgtg gcacgttctt gcatacgacg tgctacggtt tcatttatct
    31141 ccgactggaa acttcttata caaagtcgat acgccaacat cataaatgat cgccaccttc
    31201 tggcgaggaa cgcctgatgc aattaatcgc ccggcctgcg cccattgttc tggtgtaagt
    31261 ttgggacgac gtccaccaat tcgtccctgc gcgcgagcag cttccagtcc agcttttgtt
    31321 cgttcaacaa tcagttctcg ttccatttca gccagggcac ccatcacatg aaagaaaaaa
    31381 cgccccatcg gtgtgctggt atcaatagca tccgtcaggc tgcgaaaatt aacgccacgt
    31441 tcgcgcaact cctcaaccag aatgaccaga tgccgcatac tacgccccag ccgatccagc
    31501 ttccagacaa ccagagtgtc acctgccgat aatgtcctga gcagtttttt cagtcccggc
    31561 ctttcggact ttgtaccgct tatcttgtct tcaaaaatca gctcgcatcc tgcacagttc
    31621 agcgcattac gttgtagatc tgtgttctgg tcatttgttg acacacgtac atagccaata
    31681 agcatggtag atctccctga caaaagcagg aatgatgcca tttgctcgtt atttatgcat
    31741 ttttataaac
//
LOCUS       DAHBHC010000059        30589 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHBHC010000059 DAHBHC010000000
VERSION     DAHBHC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.059"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(254..460)
                     /locus_tag="M2A35_003212"
     CDS             complement(254..460)
                     /locus_tag="M2A35_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCB7199472.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            773..862
                     /gene="kdpF"
                     /locus_tag="M2A35_003213"
     CDS             773..862
                     /gene="kdpF"
                     /locus_tag="M2A35_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCB7199473.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            862..2535
                     /gene="kdpA"
                     /locus_tag="M2A35_003214"
     CDS             862..2535
                     /gene="kdpA"
                     /locus_tag="M2A35_003214"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCB7199474.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGSFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2558..4606
                     /gene="kdpB"
                     /locus_tag="M2A35_003215"
     CDS             2558..4606
                     /gene="kdpB"
                     /locus_tag="M2A35_003215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCB7199475.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4615..5187
                     /gene="kdpC"
                     /locus_tag="M2A35_003216"
     CDS             4615..5187
                     /gene="kdpC"
                     /locus_tag="M2A35_003216"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCB7199476.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5180..7864
                     /gene="kdpD"
                     /locus_tag="M2A35_003217"
     CDS             5180..7864
                     /gene="kdpD"
                     /locus_tag="M2A35_003217"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCB7199477.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSYDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7861..8538
                     /gene="kdpE"
                     /locus_tag="M2A35_003218"
     CDS             7861..8538
                     /gene="kdpE"
                     /locus_tag="M2A35_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCB7199478.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            8813..8917
                     /locus_tag="M2A35_003219"
     CDS             8813..8917
                     /locus_tag="M2A35_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCB7199479.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9228..11426
                     /gene="speF"
                     /locus_tag="M2A35_003220"
     CDS             9228..11426
                     /gene="speF"
                     /locus_tag="M2A35_003220"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCB7199480.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11423..12742
                     /gene="potE"
                     /locus_tag="M2A35_003221"
     CDS             11423..12742
                     /gene="potE"
                     /locus_tag="M2A35_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCB7199481.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12798..14438)
                     /gene="pgm"
                     /locus_tag="M2A35_003222"
     CDS             complement(12798..14438)
                     /gene="pgm"
                     /locus_tag="M2A35_003222"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCB7199482.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14464..15009)
                     /gene="seqA"
                     /locus_tag="M2A35_003223"
     CDS             complement(14464..15009)
                     /gene="seqA"
                     /locus_tag="M2A35_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCB7199483.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            15194..15958
                     /gene="ybfF"
                     /locus_tag="M2A35_003224"
     CDS             15194..15958
                     /gene="ybfF"
                     /locus_tag="M2A35_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCB7199484.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16098..16391
                     /gene="ybfE"
                     /locus_tag="M2A35_003225"
     CDS             16098..16391
                     /gene="ybfE"
                     /locus_tag="M2A35_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCB7199485.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16531..17061
                     /gene="fldA"
                     /locus_tag="M2A35_003226"
     CDS             16531..17061
                     /gene="fldA"
                     /locus_tag="M2A35_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCB7199486.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFKGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17271..17357
                     /gene="uof"
                     /locus_tag="M2A35_003227"
     CDS             17271..17357
                     /gene="uof"
                     /locus_tag="M2A35_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCB7199487.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17350..17796
                     /gene="fur"
                     /locus_tag="M2A35_003228"
     CDS             17350..17796
                     /gene="fur"
                     /locus_tag="M2A35_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCB7199488.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17880..18206)
                     /locus_tag="M2A35_003229"
     CDS             complement(17880..18206)
                     /locus_tag="M2A35_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199489.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18256..19662)
                     /gene="chiP"
                     /locus_tag="M2A35_003230"
     CDS             complement(18256..19662)
                     /gene="chiP"
                     /locus_tag="M2A35_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCB7199490.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20108..21772)
                     /gene="glnS"
                     /locus_tag="M2A35_003231"
     CDS             complement(20108..21772)
                     /gene="glnS"
                     /locus_tag="M2A35_003231"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCB7199491.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21974..23920)
                     /gene="nagE"
                     /locus_tag="M2A35_003232"
     CDS             complement(21974..23920)
                     /gene="nagE"
                     /locus_tag="M2A35_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCB7199492.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24253..25053
                     /gene="nagB"
                     /locus_tag="M2A35_003233"
     CDS             24253..25053
                     /gene="nagB"
                     /locus_tag="M2A35_003233"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCB7199493.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25113..26261
                     /gene="nagA"
                     /locus_tag="M2A35_003234"
     CDS             25113..26261
                     /gene="nagA"
                     /locus_tag="M2A35_003234"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCB7199494.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26270..27490
                     /gene="nagC"
                     /locus_tag="M2A35_003235"
                     /gene_synonym="nagR"
     CDS             26270..27490
                     /gene="nagC"
                     /locus_tag="M2A35_003235"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCB7199495.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIETCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27538..28290
                     /gene="nagD"
                     /locus_tag="M2A35_003236"
     CDS             27538..28290
                     /gene="nagD"
                     /locus_tag="M2A35_003236"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCB7199496.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28547..30211
                     /gene="asnB"
                     /locus_tag="M2A35_003237"
     CDS             28547..30211
                     /gene="asnB"
                     /locus_tag="M2A35_003237"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCB7199497.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7230 a         7740 c         8003 g         7616 t
ORIGIN      
        1 tttccgctca tctgctcatc cttgttttat cgggttattt gtctgctgac aggcgaaacc
       61 ctacagattt ataagaaagc cttaagtgag atatctgtca gataagaaca attaggaaaa
      121 tatttatata tatttcatga atatatgttt cgattggtat aacgatgtct aaatattcct
      181 ctgaacatct tcctgataca aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt
      241 caaaactcag caatcaataa aaatcaccag ttgccttttc ggcctgatcc atccacaccg
      301 gtttatcgct ggttttcgac cagacgcgat gcaggtagct gtaaaagcgg gcgcggtctt
      361 tccagaaaag catgaaaggc agcgccagaa cgcccgctgc aaccgcataa gtacggcgta
      421 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
      481 tctacccggt gaatggcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
      541 ttttgtgtaa ttttactact catccgacca cttatttttg cttattgatg gtttatttac
      601 attcatcctg taattaagtt acacaaaagt taaattaata ctaaacatta gttaaatcat
      661 ggcttttgcc atttttatac tttttttaca ccccgcccgc agatttttgc gaaatctttg
      721 cagccagaat tctacccttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc
      781 aggcgtgata accggcgtat tgctggtgtt tttattactg ggttatctgg tttatgccct
      841 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgatcgc cacgttttta
      901 ctggtgttaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
      961 attcctcttc caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct
     1021 gaccatgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg
     1081 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag
     1141 ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc
     1201 aactggcaat cttacagcgg tgaaaccacg ttgagctatt tcagccagat ggctggctta
     1261 acggtgcaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cctcatccgt
     1321 gcgtttaccc gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc
     1381 acgttatggg tgctcgtccc tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt
     1441 gcgctgcaaa actttcagcc ttatcaggct gtgaataccg ttgaaggagc gcaacagctg
     1501 ttacccatgg ggcctgtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt
     1561 agcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
     1621 gtgcagatgc tggcgatctt cttgatccca acggcgctgt gctttgcctt tggtgaagtg
     1681 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     1741 tgcgtaggcg tggtgatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc
     1801 gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     1861 ctgtttgcgg tcgtgactac ggcggcttcc tgtggcgcgg tgattgcgat gcatgattcg
     1921 tttaccgctc tcggtggcat ggtgccgatg tggctgatgc aaattggtga agtggtgttc
     1981 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     2041 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     2101 atgaaactga ctgcactggc gattctggtt accccgacgc tggtgctgat gggcgcggcg
     2161 ctggcaatga tgaccgacgc cggacgtagc gccatgctca accctggccc gcatggtttt
     2221 agcgaagtgc tgtacgccgt gtcatccgcc gctaacaaca acggcagcgc ctttgccgga
     2281 ttaagcgcca actccccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     2341 ttcggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa
     2401 cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc
     2461 accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg
     2521 gaatatctct cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct
     2581 attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca
     2641 ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg
     2701 tattagcatc gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat
     2761 tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg
     2821 ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa
     2881 gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa
     2941 aggtgacatc gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga
     3001 aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga
     3061 atccggcggc gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt
     3121 gattgagtgt agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga
     3181 aggcgcacag cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct
     3241 gaccatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa
     3301 tgccgtgagt gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg
     3361 cggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat
     3421 tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa
     3481 aaccggcacc atcacactcg gtaaccgtca ggcgtcggag tttatccctg cgcagggcgt
     3541 ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga
     3601 aggccgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca
     3661 gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat
     3721 cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccatg ttgaggctaa
     3781 cggcggtcac ttccctaccg atgttgatca aaaagtcgat caggttgcgc gtcagggggc
     3841 cacgccgctg gtggtggtgg aaggttcccg tgtgctgggc gttattgcgc tgaaagatat
     3901 cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc aaaatgggca ttaaaacggt
     3961 gatgattacc ggcgataacc gtctgactgc cgccgccatt gctgcggaag cgggcgtcga
     4021 tgattttctc gccgaagcga caccggaggc caagctggca ttgattcgtc agtatcaggc
     4081 ggaaggccgt ttggtggcga tgaccggcga cggcaccaac gatgctccag cgctggcgca
     4141 ggcagatgtc gcggtggcga tgaactccgg tactcaggcg gcgaaagaag cgggcaatat
     4201 ggtcgatctc gactctaacc cgaccaagtt gatcgaggtg gtacatattg gcaaacagat
     4261 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4321 cgccattatt ccggcggcgt tcgcggcaac gtatccgcag ttaaatgcgc tgaacatcat
     4381 gcgccttcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4441 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4501 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4561 tatcaaagtc attgatttac tgctgaccgt ttgcggtctg gtgtgaggtt taccatgcgt
     4621 ggcttacgtc cggcattatc gacatttatc tttctgttat tgattactgg cggcgtttac
     4681 ccgctgctga ccaccgtgct ggggcaatgg tggtttccct ggcaggccaa cggttcgttg
     4741 attcgtgaag gtgatacggt gcggggttcg gcattaatcg ggcagaattt taccggcaac
     4801 ggctattttc agggtcgccc gtcggcaacg gcagaaatgc cctataatcc acaggcttct
     4861 ggcgggagca atctggcggt cagtaaccct gagctggata aacaaatagc cgcacgcgtt
     4921 gctgcattac gggccgctaa cccggatgcc agtacgaacg ttccggttga actggtgacg
     4981 gcatcggcaa gcgggctgga caataatatc accccgcaag cggcggtctg gcaaatccca
     5041 cgcgtggcga aagcgcgtaa tctcagcgtt gaacagctca cgcaactgat cgcaaaatac
     5101 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt tgaactcaat
     5161 ctggcgctgg ataaacttga tgaataacga acccttacgt cccgaccccg atcgtctgct
     5221 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg
     5281 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5341 tattgtggtt ggcgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5401 gctggctgtt ctgccgccca aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5461 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5521 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5581 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5641 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5701 cgacgacgta gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5761 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     5821 tctgatcgcc ctgcgtgaac tggcactgcg ccgtactgcc gatcgcgtag atgagcaaat
     5881 gcgcgcctgg cgggggcatc ctggtgaaga gaaagtgtgg cacacgcgcg acgcgatcct
     5941 tttatgcatc gggcataaca ccggcagtga gaaactggtc cgcgcggcgg cgcggttggc
     6001 ctcgcgcctg ggtagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt
     6061 accggaaaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6121 ggagacggct acactttctg atccagcgga agaaaaagca gtagtgcgtt atgcccgtga
     6181 acataatctc ggcaagatta ttctcggtcg cccggcctcg cgccgctggt ggcgtcggga
     6241 aacgtttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6301 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctctt ttaaagacaa
     6361 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacttt
     6421 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6481 tggcgtggtg gtggtggcgc tattttatgg acgctggcct tcggtggttg ctaccgtcat
     6541 taatgtggtg agttacgatc tcttttttat cgccccacgc ggcacgctcg ctgtctctga
     6601 tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct
     6661 tactgcgggt gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca
     6721 cttatatgaa atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac
     6781 cagcgaacaa tttattgcct ccacgtttca tgcccgcagt caggtgttgt tgcccgatga
     6841 caacggtaaa ttgcagccgt taacacatcc gcaaggaatg acgccatggg acgatgccat
     6901 cgcgcagtgg agttttgata aaggcctgcc tgcgggtgcg ggcaccgaca cgttacccgg
     6961 cgtgccgtac cagattttgc cgctaaaaag cggcgagaaa acctacggac tggtggtggt
     7021 ggaaccgggg aatctgcgtc agttgatgat cccggagcag cagcgcctgc tagagacgtt
     7081 tacgctgtta gtcgccaatg cgtttgagcg gctgacgctg accgccagtg aagaacaggc
     7141 gcggatggca agcgaacgtg aacagatccg caacgccctg ctggcggcgc tttcgcatga
     7201 tttacgcacg ccgcttacgg tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc
     7261 aagcgaagga tcgccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac
     7321 tacccgactg gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt
     7381 gaagaaagag tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc
     7441 gggtttatcg tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga
     7501 cgggccactc tttgaacggg tgctgattaa tctgctggag aacgcggtga aatatgcggg
     7561 tgcgcaggcc gaaattggta tcgatgccca cgttgagggc gaaaatctac aactggatgt
     7621 ctgggataac ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc
     7681 tcgcgggaat aaagagtcgg cagtaccggg ggtagggctt ggactggcaa tttgtcgggc
     7741 gatagtggat gtacacgggg gcactattac cgcgttcaac cgaccggaag gtggtgcctg
     7801 ttttcgtgtt acacttcccc agcaaaccgc tcctgaactt gaagactttc atgaggatat
     7861 gtgacaaacg ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg
     7921 ctggagggcg acgggatgcg cgtctatgag gccgaaacgc tgcaacgcgg cttgctggaa
     7981 gcggctaccc gtaagccaga tttgattatt ctcgatctcg gcctgcccga tggtgatggg
     8041 attgagttta tccgcgacct gcgccagtgg agcgcggtgc cggtgattgt gctttccgca
     8101 cgcagcgaag agagcgacaa aatcgccgcg ctggatgccg gagcggatga ttatctgagt
     8161 aagccgtttg gcattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc
     8221 gccaccgccg cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc
     8281 cgcgtgattc accggggtga ggaagaggtg catctcacac cgattgagtt ccgcctgctg
     8341 gcggtgctgc tgaataatgc cgggaaagtg ctcacccagc gccagttact caatcaggtg
     8401 tgggggccaa acgcggtcga acacagtcac tatttgcgta tttatatggg acatctgcga
     8461 caaaaactgg aacaggatcc cgctcgcccc cgccatttca ttactgaaac cggtattggg
     8521 tatcggttta tgccatgaat gtttattcaa taaatgcgct taaataatta ttaatttatt
     8581 attaaacctg ccaaaaatat tattatttgg caggtttaat ttcttaacgc aaatataaaa
     8641 cacaaaatta caacatttaa ataacaaaac agcatctaat atggcgctat tcataattaa
     8701 tgatttatta tttggtgtat gatcgttttt tgttgatctt cttcacagat tatagccatt
     8761 tcatggatag aataactcta ccttcaactg acacagcaag aggtaaaggt aaatggaaaa
     8821 taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt ttgcccatcg
     8881 taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa gggcacccca
     8941 acgtacaggt ctccctgact ttaagcatta caggttaata cctgtattcc tcggtgctca
     9001 tatactgcta acccttttaa actctaaata attcgagtcg cagcacttgc aacttgaggt
     9061 atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa aaagcaaact
     9121 actgatttat ttatcagcgg tggagctttg ctttttttcc ggcgtgatcg atttctcctt
     9181 tgagaaattg aggacctgct attacctgaa ataaagagat gaacaaaatg tcagaattaa
     9241 aaattgccgt tagtcgttct tgcccggatt gtttttccac tcatcgtgca tgcgtgaata
     9301 tagacgaaag taattatatt gacgttgccg ccattatttt atcagtcagt gatgttgaac
     9361 gtggaaaact cgatgaaata gacgctactg gctatgacat tcctgttttt attgcaacgg
     9421 aaaatgaaga acgtgtaccc gcagaatatc tgccacgtat ttctggtgtc ttcgaacatt
     9481 gcgaatcacg taaagagttt tatggacgtc agttagaaac cgctgccagc cattatgaaa
     9541 cgcaactgcg cccgcccttc ttccgcgcac tggtcgatta tgttaatcag ggcaatagtg
     9601 cttttgactg ccctggtcat caggggggtg agtttttccg tcgccatccg gcaggcaatc
     9661 agttcgtgga atactttggc gaaatgctct tccgctctga cttgtgtaat gctgacgtag
     9721 caatgggcga tctgctgatt catgaaggtg cgccatgtat tgcacaacag catgcggcta
     9781 aagtctttaa tgccgataaa acctatttcg ttttaaacgg tacttcatct tctaataaag
     9841 tggtcttaaa cgcattgctg acgccgggcg atctggtgtt gtttgaccgc aataaccata
     9901 aatctaacca ccacggtgcg ttgctacagg ctggcgcgac accggtttat ctggaaaccg
     9961 cacgtaaccc gtatggcttt atcggtggca ttgatgcgca ctgttttgaa gaaagttacc
    10021 tgcgtgagct gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt cctttccgcc
    10081 tcgctgtgat tcagttaggc acctacgacg gtacgattta taacgcccgc caggtggtgg
    10141 ataaaattgg tcatctgtgt gactacattc tgtttgactc cgcatgggtt ggctatgagc
    10201 aatttatccc gatgatggct gactgttcgc cgctgttgct ggatcttaat gagaacgatc
    10261 cgggtattct ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac
    10321 aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca
    10381 tgaacaacgc ctttatgatg catgcctcca ccagcccgtt ctatccgctg tttgccgcac
    10441 tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg
    10501 tggtaaatgg cattaatgcc cgcaagctga tcctcgataa ctgtcagcat attcgtccgt
    10561 tcgtacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg
    10621 ttgatctgcg cttcttccag tttgtaccag gtgagcactg gcactctttt gaaggctatg
    10681 cagagaatca atactttgtc gatccatgca aactgttgct gacaacccca ggtattgatg
    10741 cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct aacttcctgc
    10801 gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc
    10861 cggcagaaga tatggccaag cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac
    10921 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
    10981 agcgctacgc cggttatacc ctgcgtcagt tgtgtcaaga aatgcatgat ttgtatgccc
    11041 gtcacaacgt gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtaa
    11101 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
    11161 tgccggatgc agaaggccgt atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc
    11221 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
    11281 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
    11341 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
    11401 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
    11461 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
    11521 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
    11581 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg
    11641 cggttatgcg gagtatgctt tcggtaaatc cggtaacttt atggcgaact atacctacgg
    11701 cgtctcgctg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
    11761 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
    11821 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
    11881 cgtgtggggg gtcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
    11941 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
    12001 ttcttccatc gctatgacgc tgtgggcttt tctcggtctg gagtctgcgt gtgcgaatac
    12061 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
    12121 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
    12181 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttca caccagaagt
    12241 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
    12301 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
    12361 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
    12421 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
    12481 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
    12541 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
    12601 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
    12661 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
    12721 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgccct
    12781 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
    12841 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
    12901 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
    12961 ccgatagaag caccattacc cggtgccgcc gtcagacgtg cggtgatcgg gtcacctgcc
    13021 agggtgctgg cgctcaccat ttccggagac agcttagaca acgccgcttt ttgtgcggaa
    13081 gtcgcagcgg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
    13141 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
    13201 atgatgccgt ctttatcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagccccg
    13261 gcgctctctt cgccgccaaa gccgaaactg ccgtcgaaca gaccatcaac aaaccatttg
    13321 aatcctaccg ggacttctac cagtttacgg cccaaggcgt tgaccacacg gtcgatcatc
    13381 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
    13441 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
    13501 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
    13561 agtgccagca ggcccgccat cgcacactcg gaggagcagt ccatacggat cgcgccgtct
    13621 ttatcaaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
    13681 aggttgtaat actcgccaat acgcttccag tattcgatac cggaaccgcc cagcggatca
    13741 acgccaagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagacct
    13801 tccacaaacg gctgcaccag atcctgctct ttcacatgac cggatgccat cgcttcgtcg
    13861 agagagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cctgtcttcc
    13921 accactttgg tgacgttggt atcagccggg ccaccatttg gcgggttgta cttgatacca
    13981 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
    14041 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa tccattgttt
    14101 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
    14161 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccatttttc
    14221 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
    14281 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
    14341 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
    14401 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
    14461 gttttagata gttccgcgaa ccttctcaat caattccgcc gggaattgca tcgactgcat
    14521 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
    14581 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
    14641 tgccgcaaag taaacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
    14701 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
    14761 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
    14821 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
    14881 agcagcaggc tgtgatgagg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
    14941 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
    15001 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccag gagtatatga
    15061 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
    15121 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
    15181 aaagaacttt gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc agcacaataa
    15241 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
    15301 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
    15361 accgagagat ccggtaatga attacccggc gatggcgcag gatcttgttg atactctgga
    15421 tgcacagcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
    15481 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
    15541 ggtcgactat cacgtacgcc gtcatgatga gattttcgcg gctatcaacg cggtcagtga
    15601 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
    15661 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
    15721 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
    15781 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
    15841 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
    15901 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
    15961 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
    16021 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
    16081 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
    16141 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
    16201 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
    16261 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggagtcg cgcaatatga
    16321 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
    16381 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
    16441 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
    16501 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
    16561 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
    16621 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta tgacattctg
    16681 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
    16741 ccgactctcg aagagattga tttcaagggc aaactggttg cgctgtttgg ttgtggtgac
    16801 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
    16861 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
    16921 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
    16981 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
    17041 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
    17101 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa
    17161 tgtcccggtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
    17221 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
    17281 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
    17341 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
    17401 ccacgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
    17461 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
    17521 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
    17581 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
    17641 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
    17701 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
    17761 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
    17821 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
    17881 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
    17941 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
    18001 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
    18061 gcttcaattt tttctggcga accttgtgcg gtgttgatac aggcgctgta tgcctctttc
    18121 agacgactgt cttcctgtgg cgcggttgat tgcgcacaag ctaccagccc cgatgccatt
    18181 atggcgatga gaatcagttt tttcatagca aattcctcaa acgtgcggca ggcacaaaac
    18241 ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg
    18301 ctcatcctgg aagatgttac cgtaaccgcc gccccagctt gggatgtcag agtggttgtc
    18361 gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta
    18421 gaccgcatcc aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc
    18481 cggattgctc tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat
    18541 ggcgaagcct ggaagattcc agtttttcag gtcatacatc gcaccgaaga agaccgcttt
    18601 ttcgccgttg gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga
    18661 ggaagcgtag gttggagtca tacgttgcag gaagtatccc tgctgaccgt cagccttaac
    18721 ccaggtgcct tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc
    18781 ttgcagccag gcggtgccgt cataaaggtc gttgacgctg cgatcgtcaa ctttatcgcg
    18841 cgtaccatag aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct
    18901 ggctttggca aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac
    18961 gaagttattt ttgaagtcgt atttcgcacc gatggagtgc aggtaatcaa ctttggtggt
    19021 tttatcgttc tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt
    19081 ccacatgtag gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc
    19141 cgccccctga taagtacctg gcataaagct ccagtgcggc gctaacagcg tttgaccagt
    19201 tggctgaatg taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt
    19261 atacaggctt ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt
    19321 tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt
    19381 aaacgcagca atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt
    19441 ggcattccag gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt
    19501 acggtcgcgt tcacgctgcc agtaatatat accgccggtt aaggttgaat cgtcgatgaa
    19561 tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat
    19621 agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac
    19681 gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa aaattaaaaa agtagcgttg
    19741 agaaaagtct cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct
    19801 ttctgtaacc agaatgtaag aacatgagca accgcacata ttttgatgat ttttgttaca
    19861 aggcttaagt ttgcggttga aaggcttgca gaagaataaa agcgtgaatt tccagcaaga
    19921 tgaaagcggt tacattttat ttaattatgg tttgtgattt taaatttatt ttatggataa
    19981 attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa acgccgcaga gtgcggcgtt
    20041 tgggattgtt tgctatttct tgccggatgc gacgtaaatg cctcacccgg cctggtagaa
    20101 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20161 ccggtttttc cgctgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact
    20221 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20281 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20341 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20401 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20461 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tcagccttaa
    20521 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20581 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctgac
    20641 gactccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct
    20701 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt
    20761 cgttgagatc ttcacggatg caggattcca gcgatgccat ctcaatggtg ttgtcctgct
    20821 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    20881 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    20941 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21001 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21061 ggttgtcctg aaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21121 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21181 cagcaaattt aatacggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21241 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21301 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21361 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21421 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21481 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21541 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21601 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagataac
    21661 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21721 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21781 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    21841 tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa
    21901 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca
    21961 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    22021 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    22081 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    22141 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    22201 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    22261 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    22321 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    22381 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    22441 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    22501 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    22561 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    22621 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    22681 tgatacaggc gtcaattgct ttcaggttgt cagtgccgcc aaccgcagca atatagttgg
    22741 tcgccagttg attgaggcct tcttcagtgt tgctgttggc ttcttcagta acgatctcgt
    22801 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    22861 ccacgaagta gatagcgaag aagacaacac ccataaccag cagcatccag acgttctggc
    22921 tggcggccgg caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc
    22981 cagcatggat acccagcagt gttgccacaa acaggctgat accggtcagc agtgcgtgca
    23041 ggaggtacag cagcggtgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    23101 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    23161 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc ataatcggga
    23221 agaagccgga catgaacatc cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac
    23281 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    23341 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    23401 aacccagcgc gcccgcagaa acgatccact cgcctcctgc atggatagcg tgctgtaccg
    23461 gcggccagac gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg
    23521 gcacaaagcg tttgccgccg aagaaactca ggaagtccgg cagtttaata tcggaccaac
    23581 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    23641 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    23701 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    23761 atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    23821 ctggctgacc gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta
    23881 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    23941 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc
    24001 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    24061 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    24121 ctggctaaag cagaaaactt attttatcat tcaaaaaatc aggtcggatt gacgcctgtc
    24181 tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta
    24241 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    24301 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt
    24361 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    24421 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    24481 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    24541 gttgatattc cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc
    24601 gaatgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    24661 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    24721 actcgtatca aaaccttgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    24781 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc
    24841 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt
    24901 gaaggttgcg tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc
    24961 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    25021 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct
    25081 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    25141 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    25201 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    25261 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    25321 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    25381 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    25441 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    25501 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    25561 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    25621 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    25681 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    25741 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    25801 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatt gctgatggcc
    25861 tgcatgttga ttacgccaac attcgtaacg ctaaacgtct gaaaggcgac aaactgtgtc
    25921 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    25981 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    26041 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    26101 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg
    26161 gcacactcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    26221 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    26281 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    26341 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    26401 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    26461 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    26521 caatttccac gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga
    26581 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac
    26641 gctggaacat gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact
    26701 gcgcgagctg atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    26761 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactggaaga
    26821 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    26881 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    26941 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggca acgtcggtga
    27001 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    27061 cctggaaact atcgctgcca acgccgctat tgaacaacgg gtgttgaatc tgttaaagca
    27121 gggctaccag agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc
    27181 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggcaa
    27241 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat
    27301 caccgaagcc gataaagtgc tgctccctgc tattgaaacc tgcattaata cccaggcgct
    27361 gaaggcgttt cgcactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    27421 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    27481 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    27541 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    27601 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    27661 accaactatc cttcgcagac cgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    27721 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ctgccgattt cctgcgtcgc
    27781 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    27841 gccggtttca ctattaccga tgtgaaccct gattttgtga ttgttggcga aacgcgttcc
    27901 tacaactggg acatgatgca taaagcggcc tatttcgtcg ctaacggtgc acgctttatc
    27961 gccaccaacc cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca
    28021 gggattgaga aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc
    28081 atccgcgcgg cattaaacaa aatgcaggcg cattcggaag aaacggtgat tgtcggcgat
    28141 aacttgcgca ccgacattct ggcgggcttc caggcgggtc tggagacgat tctggtgctt
    28201 tctggtgttt catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac
    28261 ccgtcggtcg ctgaaatcga cgttatctga atagcaacca cgtctcaggg cgtggtttct
    28321 tatttttacc catcattaaa atcatcccat tcaataaaac tcgccaaaaa ttatcgatcc
    28381 atcaataaat acatcaccac accctcccct ttttcacaaa tcagcaatca ccattacgtt
    28441 ttttattttt gcactgccaa atcgcttgcg cacggtgctg ctttgcggca tttttttaaa
    28501 caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt
    28561 tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc
    28621 acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat
    28681 tctcgcccac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa
    28741 ccaacaaaaa actcatgtgc tggcggtaaa cggtgaaatc tacaaccacc aggcactgcg
    28801 cgccgaatat ggcgatcgtt atcagttcca gaccggatct gactgcgaag tgatcctcgc
    28861 gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc
    28921 cctgtacgac agcgaaaaag atgcatatct gattggtcgc gaccatctgg ggatcatccc
    28981 actgtatatg ggctatgacg agcacggtca gctgtatgtg gcctcagaaa tgaaagccct
    29041 ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagtcagga
    29101 cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa
    29161 cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa gccatctgat
    29221 gtctgacgtt ccttacggtg tgctgctctc gggtggtctg gattcctcaa ttatttccgc
    29281 gatcaccaag aaatacgccg cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg
    29341 gccgcaatta cactcctttg ctgtaggtct gccgggttca ccggatctga aagcagccca
    29401 ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg
    29461 tctggatgcc atccgcgacg tgatttacca catcgaaacc tatgatgtga caactattcg
    29521 cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcaatgggca ttaaaatggt
    29581 gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc acaaagcacc
    29641 gaatgccaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga
    29701 ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct
    29761 cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa
    29821 cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt
    29881 ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct
    29941 gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt
    30001 cccgtacaac acgccaacct caaaagaagc gtatctgtac cgggagatct ttgaagaact
    30061 gttcccgctt ccgagcgccg ctgagtgcgt gcctggcggt ccttccgtcg cgtgttcttc
    30121 cgctaaagcg attgagtggg atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc
    30181 ggttggcgtt caccagtcgg cgtataagta agaaatgcga aacagccccg gtgaaatccg
    30241 gggctgtttc agttattgag gtgcttgtta attctgatat gcagtaagga aatgttccgg
    30301 gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt
    30361 cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg gctgtagggt
    30421 ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct
    30481 aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct
    30541 atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaag
//
LOCUS       DAHBHC010000060        30559 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHBHC010000060 DAHBHC010000000
VERSION     DAHBHC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.060"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..174)
                     /locus_tag="M2A35_003238"
     CDS             complement(<1..174)
                     /locus_tag="M2A35_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7199498.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQT"
     gene            240..1328
                     /locus_tag="M2A35_003239"
                     /pseudo
     CDS             240..1328
                     /locus_tag="M2A35_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1664..2641
                     /gene="csiD"
                     /locus_tag="M2A35_003240"
     CDS             1664..2641
                     /gene="csiD"
                     /locus_tag="M2A35_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCB7199499.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            2661..3929
                     /gene="lhgO"
                     /locus_tag="M2A35_003241"
     CDS             2661..3929
                     /gene="lhgO"
                     /locus_tag="M2A35_003241"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCB7199500.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTSQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRSLRAARSVDALHAAFNQ"
     gene            3952..5400
                     /gene="gabD"
                     /locus_tag="M2A35_003242"
     CDS             3952..5400
                     /gene="gabD"
                     /locus_tag="M2A35_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCB7199501.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5414..6694
                     /gene="gabT"
                     /locus_tag="M2A35_003243"
     CDS             5414..6694
                     /gene="gabT"
                     /locus_tag="M2A35_003243"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCB7199502.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            6932..8332
                     /gene="gabP"
                     /locus_tag="M2A35_003244"
     CDS             6932..8332
                     /gene="gabP"
                     /locus_tag="M2A35_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCB7199503.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8353..9015
                     /gene="csiR"
                     /locus_tag="M2A35_003245"
     CDS             8353..9015
                     /gene="csiR"
                     /locus_tag="M2A35_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCB7199504.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9016..9465)
                     /gene="kbp"
                     /locus_tag="M2A35_003246"
     CDS             complement(9016..9465)
                     /gene="kbp"
                     /locus_tag="M2A35_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCB7199505.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9549..9707)
                     /locus_tag="M2A35_003247"
     CDS             complement(9549..9707)
                     /locus_tag="M2A35_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCB7199506.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9890..10189
                     /locus_tag="M2A35_003248"
     CDS             9890..10189
                     /locus_tag="M2A35_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7199507.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10199..10723
                     /gene="ygaP"
                     /locus_tag="M2A35_003249"
     CDS             10199..10723
                     /gene="ygaP"
                     /locus_tag="M2A35_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCB7199508.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(10770..11174)
                     /gene="stpA"
                     /locus_tag="M2A35_003250"
     CDS             complement(10770..11174)
                     /gene="stpA"
                     /locus_tag="M2A35_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCB7199509.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            11842..12291
                     /gene="alaE"
                     /locus_tag="M2A35_003251"
     CDS             11842..12291
                     /gene="alaE"
                     /locus_tag="M2A35_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCB7199510.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12328..12672)
                     /locus_tag="M2A35_003252"
     CDS             complement(12328..12672)
                     /locus_tag="M2A35_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCB7199511.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            12824..13153
                     /locus_tag="M2A35_003253"
     CDS             12824..13153
                     /locus_tag="M2A35_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCB7199512.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            13401..13646
                     /gene="nrdH"
                     /locus_tag="M2A35_003254"
     CDS             13401..13646
                     /gene="nrdH"
                     /locus_tag="M2A35_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCB7199513.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            13643..14053
                     /gene="nrdI"
                     /locus_tag="M2A35_003255"
     CDS             13643..14053
                     /gene="nrdI"
                     /locus_tag="M2A35_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCB7199514.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            14026..16170
                     /gene="nrdE"
                     /locus_tag="M2A35_003256"
     CDS             14026..16170
                     /gene="nrdE"
                     /locus_tag="M2A35_003256"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCB7199515.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            16180..17139
                     /gene="nrdF"
                     /locus_tag="M2A35_003257"
     CDS             16180..17139
                     /gene="nrdF"
                     /locus_tag="M2A35_003257"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCB7199516.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            17494..18696
                     /gene="proV"
                     /locus_tag="M2A35_003258"
     CDS             17494..18696
                     /gene="proV"
                     /locus_tag="M2A35_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCB7199517.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            18689..19753
                     /gene="proW"
                     /locus_tag="M2A35_003259"
     CDS             18689..19753
                     /gene="proW"
                     /locus_tag="M2A35_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCB7199518.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            19811..20803
                     /gene="proX"
                     /locus_tag="M2A35_003260"
     CDS             19811..20803
                     /gene="proX"
                     /locus_tag="M2A35_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCB7199519.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            21095..22279
                     /locus_tag="M2A35_003261"
     CDS             21095..22279
                     /locus_tag="M2A35_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199520.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            22403..23140
                     /gene="ygaZ"
                     /locus_tag="M2A35_003262"
     CDS             22403..23140
                     /gene="ygaZ"
                     /locus_tag="M2A35_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCB7199521.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            23130..23465
                     /gene="ygaH"
                     /locus_tag="M2A35_003263"
     CDS             23130..23465
                     /gene="ygaH"
                     /locus_tag="M2A35_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCB7199522.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            23556..24086
                     /gene="emrR"
                     /locus_tag="M2A35_003264"
     CDS             23556..24086
                     /gene="emrR"
                     /locus_tag="M2A35_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCB7199523.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHLLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            24213..25385
                     /gene="emrA"
                     /locus_tag="M2A35_003265"
     CDS             24213..25385
                     /gene="emrA"
                     /locus_tag="M2A35_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCB7199524.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            25402..26940
                     /gene="emrB"
                     /locus_tag="M2A35_003266"
     CDS             25402..26940
                     /gene="emrB"
                     /locus_tag="M2A35_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCB7199525.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(27004..27519)
                     /gene="luxS"
                     /locus_tag="M2A35_003267"
     CDS             complement(27004..27519)
                     /gene="luxS"
                     /locus_tag="M2A35_003267"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCB7199526.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(27669..29225)
                     /gene="gshA"
                     /locus_tag="M2A35_003268"
     CDS             complement(27669..29225)
                     /gene="gshA"
                     /locus_tag="M2A35_003268"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCB7199527.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(29298..29726)
                     /locus_tag="M2A35_003269"
     CDS             complement(29298..29726)
                     /locus_tag="M2A35_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCB7199528.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(29723..30289)
                     /gene="yqaB"
                     /locus_tag="M2A35_003270"
     CDS             complement(29723..30289)
                     /gene="yqaB"
                     /locus_tag="M2A35_003270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCB7199529.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         7320 a         7791 c         7823 g         7625 t
ORIGIN      
        1 cgtctgctga ttttgctgtt tactgcgcca gttgtagagc tgtgattcat acaggctgag
       61 ttcacgggct gcggcagtaa caccgatgcg ttcagcaagc ttcagggctt cactgcgaaa
      121 ttcaggcgaa tgctgtttac ggggtttttt actggttgat actgtttttg tcatgtgagt
      181 cacctctgac tgagagttta ctcacttagc cgcgtgtcca ctattgctgg gtaagatcag
      241 aatgggaaaa cctccatgca ttaaaagaaa agcgtttaaa tggaatgcct aaaacaaccc
      301 ccaaaagtga ccaggttatt atgctgcaaa atcaatacgt tcgtgaaatg cgaaaatatg
      361 gcgtacgagg tttacgttac gatgcagcaa aacactcaaa acatgaacaa atagaaagat
      421 caataacccc accgcttaaa aattataatg agcggttaca caatactaat ttatttaacc
      481 caaaatatca tgaaaaagtc gttatgaatt acatggaata tctggtaact tgtcagttgg
      541 atgaagaaca aatgtcatcg ctgctttatg aaagagatga tttaagcgcc attgattttt
      601 cattgctcat gaagacgata aaagcttttt catttggtgg cgatctccaa acccttgcat
      661 caaaaccggg ttccacaatc tcaagcatcc catcaaaaag acggatattg attaacatta
      721 aacacgattt tcctaacaat ggcaatattt tcaatgactt tctatttaac catcaacaag
      781 atgaacaatt agcaatggca tatatggccg ctctcccgtt cagcaggcct ttagtttact
      841 gggatggcca agtattaaaa tcaacgactg aaattaaaaa ttatgatggg tcgacgcgtg
      901 ttggcgatga ggcgtggctt aataaaggtt gctctaccta tcagcagctc tacaatgaat
      961 tccacgcatt atatatagat aaagcaggaa tatggagcgc atttgagggt gtatttgcaa
     1021 ctaaaaacgt tctggccttt agtcgtgggg attctgtgaa cattaatcac tctcctcatg
     1081 atggactaat tataataaat aaaggaaacg aagaagttga aggtgcctgg cctaacaaat
     1141 tgcaacctgg aaaatataaa aacatgggga gtaatagcgt taacattatt attattaata
     1201 cccgaaaaat tatcccccct ggtaaagcat ttatgcttag aggcggaact ctaaatatca
     1261 atattcctgg gcgtagcgct cttcttttag ggaaaacagg agaaccgctg aactatctct
     1321 atttgtaatt taatgttata tctgccccgc taaaacgggg cagataatat gtttagttta
     1381 ctaacggtca ttttgcattg aagccattta ctgtttttta tcgaccagat aatctgttct
     1441 ctgatggtaa ctccccctaa cctattgctt tagttattca tttcctgtct cactttgcct
     1501 taatacccta cgttaaatgt tactaatttg ttgcttttga tcacaataag aaaacaatat
     1561 gtcgcttttg tgcgcatttt tcagaaatgt agatattttt agattatggc tacgaaatga
     1621 gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg cactgaccgc
     1681 cgtacaaaat aacgctgtcg attcaggcca ggactatagc ggattcactt tcatcccgtc
     1741 ggcgcaatcc ccgcgtctgc tggaacttac cttcaccgaa cagacgacca aacagtttct
     1801 cgaacaggtt gccgaatggc ccgtacaggc gctggagtac aaatcctttc tgcgttttcg
     1861 ggtaggcaaa attcttgacg atctgtgtgc gaatcagctg caaccattgc tgttgaagac
     1921 cctgctaaac cgtgctgaag gtgcgctgtt gatcaatgcg gtgggtatcg atgatgtggc
     1981 gcaggcagat gagatggtga agctggcaac ggcggtagcg catctgattg gtcgctcaaa
     2041 tttcgatgcg atgagcggtc agtattacgc gcgtttcgtg gtgaaaaata tcgataactc
     2101 agacagctat ctgcgtcagc cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt
     2161 cgaagagatc accgattacg tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg
     2221 aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt tccgccatcc
     2281 actggcgcgt cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt
     2341 tttccatcca gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca
     2401 gttcgtccag ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat
     2461 tgaaaccagc aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa
     2521 caacctattc tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga
     2581 actgatgcgt cagcgtggct atttcgctta cgcctctaac cactaccaga cgcatcagta
     2641 agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca
     2701 tgtcgaccgc catgcaactg attgatgtct atccggatgc ccgcattgcg ttgctggaaa
     2761 aagagtccgg cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg
     2821 tctattacac gcccggtagc ctgaaggcac agttttgcct ggcgggaaac cgcgccacta
     2881 aagccttttg cgatcaaaac ggcattcgct ttgacaactg cggcaagatg ctggtcgcca
     2941 cgtctccgct cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta
     3001 tcgagcgcga atggctaaac gccgacgaac tgcgcgagcg cgaaccgaat atcaccgggc
     3061 tcggcggtat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acagcggcga
     3121 tggcaaaaat ctttcaggac agaggcggcg agattattta taacgccgaa gtcagcgccc
     3181 ttaatgagca taaaaacggc gtggtgatac gtaccagtca gggcggtgaa tatgaagcat
     3241 cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggactcg
     3301 aaccgggctt tatcatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata
     3361 accagattgt taaccacctg atttacccca tccccgaccc ggcaatgccg tttttgggcg
     3421 tgcatctcac ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt
     3481 tcaaacgtga aggctatcgc aagcgcgact tctcatttag cgacacgctg gaaattttgg
     3541 gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga
     3601 aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt
     3661 cgttaagcga tctccagccc tggcccgccg gtgtgcgggc gcaggcggta tcgccggacg
     3721 gcaagctgat tgacgatttt ctgtttgtca ccaccccgcg cacgatccac acctgcaatg
     3781 cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac
     3841 aaacgctgtt ggcaagccag agtaaccccg gacgctcgct gcgagcggca cgtagtgtgg
     3901 atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt
     3961 aacgacagta acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac
     4021 aatggcgagg tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg
     4081 aaaatgggcg cggatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgccagcc
     4141 tggcgcgcgc tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg
     4201 atggagcatc aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct
     4261 gaagcgaaag gcgaaatcag ctacgccgct tcctttattg agtggtttgc cgaagaaggc
     4321 aaacgcattt atggcgacac cattcccggt catcaggccg ataagcgcct gattgttatc
     4381 aagcagccga ttggcgttac cgccgccatc acgccgtgga acttcccggc ggcgatgatt
     4441 acccgtaaag ccggtccggc gctggcggca ggctgcacga tggtgctgaa acccgccagt
     4501 cagacgccgt tctctgcgct ggcgctggcg gagctggcga ttcgcgcggg cattccggct
     4561 ggcgtattta acgtggtcac cggttcggcg ggcgcggtgg gtaacgaact gaccagcaac
     4621 ccgctggtgc gcaaactgtc gtttaccggt tcgaccgaaa ttggccgcca gttaatggaa
     4681 cagtgcgcga aagacatcaa gaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc
     4741 gtctttgatg atgccgatct cgacaaagcc gtggaaggcg cactggcctc gaaattccgc
     4801 aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac
     4861 cgctttgccg aaaaattaca gcaggcggtg agcaaactgc acatcggcga cgggctggat
     4921 aaaggcgtca ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat
     4981 attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc ggatgaacgc
     5041 ggcggcaact tcttccagcc gaccattctg gtggacgttc cagccaacgc caaagtgtcg
     5101 aaagaagaga cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg
     5161 attgcgcaag ccaatgacac cgagtttggc cttgccgcct atttctacgc ccgtgattta
     5221 agccgcgtct tccgcgtggg cgaagcgctg gagtacggca tcgtcggcat caataccggc
     5281 atcatttcca atgaagtggc cccgttcggc ggcattaaag cctcgggtct gggtcgtgaa
     5341 ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa
     5401 ctggagaacg cgaatgagca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc
     5461 ccgtggtgtt gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg
     5521 ggacgttgaa ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg
     5581 gcacttgcat ccgaaagtgg tggctgcggt ggaagcgcag ttgaaaaaac tgtcgcacac
     5641 ctgcttccag gtgctggcct acgagccgta tctggagctg tgcgagatta tgaatcagaa
     5701 ggtgccaggc aattttgcca agaaaacgct gctggtcacc acaggttctg aagcggtgga
     5761 aaacgcggtg aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg
     5821 cgcgtatcac gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc
     5881 tgcgggcatg gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca
     5941 cggcataagc gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc
     6001 gccggaagat atcgccgcca tcgtgattga gccggttcag ggcgaaggcg gtttctacgc
     6061 ctcgtcgcca gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct
     6121 gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca
     6181 gatgggcgtt gcgccggatc ttaccacctt tgcgaaatcg atcgcgggcg gcttcccgct
     6241 ggcgggcgtc accgggcgcg cggaagtaat ggatgccgtc gctccaggcg gtctgggcgg
     6301 cacctatgcg ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgtttga
     6361 gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagttgaaag acggattgct
     6421 ggcaatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctggggg cgatgatcgc
     6481 cattgagctg tttgaagacg gcgatcacaa caagccggac gccaaactca ccgccgagat
     6541 cgtggctcgc gcccgcgata aaggcctgat tcttctctcc tgcggcccgt attacaacgt
     6601 gctgcgcatc cttgtaccgc tcaccattga agacgctcag atccgtcagg gcctggagat
     6661 catcagccag tgttttgatg aggcgaagca gtagcgccgc tcctatgccg gaggcgacgc
     6721 tgcgcgtctt gtccggccta cggggatcca ggtcggataa ggcgtttacg ccgcatccga
     6781 caatctgtac gtgaacagga agaaatctat gttggccggg taaggcggag ccgctctccg
     6841 gcaaaaagaa tcaataacaa ttatacgcgt gacccggcgc gggaaatgtc ggggcgctct
     6901 cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac cacatgagtt
     6961 aggcggcggg ctgaagtcac gccacgtcac catgttgtct attgccggtg ttatcggcgc
     7021 aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg ggcccggcgg tattactggc
     7081 ctatctgttc gccgggctac tggtggttat gattatgcgg atgttggcgg aaatggcggt
     7141 tgccacgccc gataccggtt cgttttccac ctatgccgat aaagccattg gtcgctgggc
     7201 gggctatacc atcggctggt tgtactggtg gttttgggta ctggttatcc cgctggaagc
     7261 caacatcgcc gccatgatcc tgcactcatg ggttccaggc attcccatct ggttattttc
     7321 cctcgtcatt accctcgcct taactggcag taacttatta agcgttaaaa actacggcga
     7381 atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg
     7441 tgcagtcgca attagcggtt tttacccgta tgccgaagtg agcgggatct caagattgtg
     7501 ggatagcggc ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac
     7561 catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacacgcc
     7621 ggaaaaacat attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt
     7681 atgttctatt tttgtcgtcg tggcattaat tccgtggaat atgcccggac taaaagccgt
     7741 cggttcttat cgctcggtac tggaattgct caatattccc catgcgaaat taatcatgga
     7801 ctgcgtgata ttactttccg taaccagttg cctgaactcg gcgctgtata ccgcgtcaag
     7861 gatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg
     7921 cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcggcattct taacggtggt
     7981 ggtgaactat tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat
     8041 cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg
     8101 agcagaagga agcgaaattc gcttgcgaat gtggctttat ccgtggctca cctggctagt
     8161 catcggcttt attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga
     8221 agtgatctcc accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg
     8281 ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gatcatgatc
     8341 aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt
     8401 attcgcggta attttcaacc ggatgaaaaa ttacgaatga gtttgctgac atcgcgttat
     8461 gcacttggcg ttgggccgtt acgggaagct ctttcgcaac tggtggcgga acggctggtc
     8521 acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat
     8581 attttcgacg cccgcgccaa tatggaagcg atgttagtga gtctggcgat tgcccgcggt
     8641 ggcgatgagt gggaggcaga cgttctcgca aaagcgcatc tgctgagtaa gcttgaggcc
     8701 tgtgacgcca gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcatacg
     8761 gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat
     8821 ctggcggcgc gctatcgatt tatctggctg cggcgaacgg tgctttcggt agaaatgctg
     8881 gaggataaac acgatcagca ccagaccctg actgcggcgg tactggcgcg agataccgcg
     8941 cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg
     9001 atggctggca attaactact cttccggaat acgcaacact tgccccggat aaattttatc
     9061 cgggcttttt agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc
     9121 gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct
     9181 ggcagtggct ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt
     9241 cccaaccgca acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt
     9301 cgctttgccg tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct
     9361 cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc
     9421 ccagagtttt tctccggcat ctttcacaaa attgaacaga cccatagcta cctcattcgt
     9481 taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga
     9541 gcacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca
     9601 agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg
     9661 ccgagcggcg gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat
     9721 aagaaaatta ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg
     9781 agcctgccag tttcagctta cgcttttact ctcatttacg ctgctgaggc tggtatatgt
     9841 tgcattttta gtttagagtt tacttaattt agaaaatact taaataaata tgactgaact
     9901 cgcgcaatta caggccagtg ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc
     9961 taaacgattg ctgattctgt gcatgcttag cggttccccc ggcaccagcg cgggagagct
    10021 gacgcgcatt accggactga gtgcctctgc gacatcacag catctcgctc gtatgcggga
    10081 cgaagggctt atcgacagcc aacgggatgc ccaacgcatt ctatattcca ttaaaaatga
    10141 ggcggtaaat gccattatcg ccaccctgaa aaatgtctat tgtccgtaag gagtcactat
    10201 ggctttgaca accatttcgc cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt
    10261 aatcgatatt cgtgacgctg atgaatatct tcgtgaacat attcctgaag cagatctggc
    10321 tccattatcc gtgctggaac agtcaggtct tccacctaaa ttacgtcgcg agcaaattat
    10381 attccactgc caggcaggta aacgcaccag taataacgcc gataaattag cagcgattgc
    10441 cgcccccgca gaaatatttt tactcgaaga tgggattgat ggctggaaaa gagctggatt
    10501 gccagtagcg gtaaataaat ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc
    10561 gggtggttta atattaatcg gcgttgtact gggttatacc gtaaatagcg gtttcttctt
    10621 attaagtggc tttgttggtg ccgggttact gtttgcagga atcagcggtt tttgtggaat
    10681 ggcaaggttg ttagataaga tgccgtggaa ccaacgaact tgagaagcga cgccggacgc
    10741 gccctagcag cgacatccgg cctcagtaat tagatcagga aatcgtcgag agatttacct
    10801 tctgccagcg cctgagcaat tggcttcggt gtacggccct gaccggtcca ggttttagtt
    10861 tcaccgttaa catcggtgaa tttatatttc gccggacgcg gctggcgttt tttaccagcg
    10921 cgtggtgcag cagcagagct attacctaat aactcttccg ggttaattcc gtcagccttc
    10981 atcagctcca gccaggtgct aattttttcc tgacgctcag ccagttcacg ctgctgctgt
    11041 tcttcttctt cacgtctttc tttagtgaca accctgaatt tttcgagcat ttcttcaaga
    11101 acgtcaatgg agaattcgcg agccatcgca cggagagtgc gaatgttatt taaactttgt
    11161 aacattacgg acataataaa gaaaaccttt taacgccaaa acaaaaaagt atttcgtcgc
    11221 gtagtgtaag cgatcactta ttatatttcc acaaaattta atctatttac agtcctgtgc
    11281 gagattattt cagctgttat tttctgaaac tctcagaata atttcaagat tatatattag
    11341 ccattttgat gagtaattta gattattggt gaacagaacg gcgctctctg ctcgctaatt
    11401 ccgcaagcct gacaaaaccg aagtgaataa aaatgcaata taacagaata aaagaaaaca
    11461 cccctcactg gtgctttctg ctgttaatgt acattttctg gcataaaaaa tactgtaaat
    11521 gacacggtca cgacatacac cgaattctct acgcttatag cgttacctca cccccaaact
    11581 ttcattagtg aaattaaagg aaaggtcata tctaattgat ttaattgaaa ataaatcata
    11641 tcggcatgta aaaatccaca atgtacaaaa aacgagccgt tacggaatat tttatctaca
    11701 aaaactgact aaataaaaat ttttcactaa ttgaatagtc atagccagcg atatacgcta
    11761 tgcgaaaatg cagatggcaa tgagatccac tgctttcatc tccattaaca tcccattacg
    11821 cttttattaa ggagcattag catgttctca ccgcagtcac gcttgcgtca tgcagttgca
    11881 gatacgttcg cgatggttgt ttactgttct gtcgtgaaca tgtgtattga agttttcctc
    11941 tccggaatga gcttcgaaca gtctttttat tccagattgg tagcgattcc ggtgaacatc
    12001 ttaattgcat ggccatacgg tatgtaccgc gatctgttta tgcgcgcggc acgcaaagtt
    12061 agcccgtcgg gctggataaa aaatctggct gatatcctgg cttatgtgac gttccagtca
    12121 ccggtgtatg tggcgatctt gttagtggtg ggcgcagact ggcatcagat tatggcggcg
    12181 gtcagttcaa acatcgttgt ttcgatgttg atgggggcgg tttatggcta cttcctcgat
    12241 tattgccgcc gactgtttaa agtcagccgt taccagcagg taaaagcctg actcttcttt
    12301 tcgcgactgg catcgccagt cgcgtcttta actggcttcg ccaaataaac cattcaaata
    12361 acgttcaagc gcaacacgcg aactaaagcc atgcgggata ccgtaatgct cgtgtcgctc
    12421 gcctgctaaa tagagcggaa attgctggta atgatcgcaa gttttaatat cggaagcggg
    12481 ttcagccagt gttgaactgc gttcttttaa tgttggatga attaccagag cggtacgtcc
    12541 cattcgcgct tcgcgattaa catagacata attttcccca cggcggtaac catacgcttt
    12601 ctgggttacc acatcgacag taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg
    12661 tcgtaaatac atatttgatc ctcgttatta tcaaccgcgg gcctacctta cctgattgcg
    12721 cattcacaac gctttcagaa aagtccataa agccgcgatg aacctcagtt aagaaaatta
    12781 tggtctacac tgaaaattac atcgaattct aatgggggat catatgttta accgaccgaa
    12841 ccgcaacgac gttgatgatg gcgtgcagga tatccagaat gatgtcaatc aattagctga
    12901 cagcctcgaa tctgtattga agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc
    12961 acgcagtaaa gctcaggcat tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg
    13021 cgtccagcaa gccgcacgcg atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc
    13081 ctggtgtagc gtgggtacag cagctgcggt aggtattttt atcggcgcac tgttaagcat
    13141 gcgcaaatcg tagtgcaaaa atgataataa atacgcgtct ttgaccccga agcctggctt
    13201 cggggtttct ttttgcctgg tgaatcacaa aaatccccct accccgtcac gctcatatcc
    13261 agggtaattt cgaccactat ttgctatata ttgtgtggtt gaatcttttt tcaactacat
    13321 ctagtatctc tgtatcaaca gagatacaac ccgacgcgta tcatcgcgca gtatcttcat
    13381 tttaaacgga aatacgaatc atgcgcatta ctatttacac tcgtaacgat tgcgttcagt
    13441 gccacgccac caaacgggcg atggaaaacc ggggctttga ttttgaaatg attaatgtcg
    13501 atcgcgttcc tgaagcagcg gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag
    13561 tgattgctgg cgatcttagc tggtctggtt tccgtccgga catgattaac cgtctgcatc
    13621 cagcgccaca cgcggccagt gcatgagcca gctcgtctac ttctccagca gctccgaaaa
    13681 cacgcagcgt tttatcgaac gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg
    13741 ggaacggatt caggtagacg agccttacat cctgatcgtg ccctcttacg gcggcggcgg
    13801 tacggctggc gcggtgccac gacaggtaat tcgcttttta aacgacgagc acaaccgggc
    13861 gttgcttcgc ggcgttattg cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc
    13921 cggagatgtg attgcccgga aatgcggcgt gccgtggctg taccgttttg aactcatggg
    13981 tacgcaaagc gatatcgaaa acgttcgtaa aggagtaacc gaattttggc aacgacaacc
    14041 gcagaatgcc tgacgcagga aacgatggat taccacgcgc tgaatgcgat gcttaacctc
    14101 tacgatagcg caggtcgcat tcagttcgat aaagaccgcc aggccgttga cgcctttatt
    14161 gcgacgcatg tgcgtccgaa cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg
    14221 ctggtcaacg aaggttacta tgatgaaagc gttcttaatc gctactctcg cgactttgtc
    14281 attacgctgt ttgcccacgc acacaccagc ggttttcgtt tccagacatt cctcggggca
    14341 tggaagtttt acaccagcta tacgttgaag acattcgacg gtaaacgtta tctggaagat
    14401 tttgccgatc gagtcacgat ggtggcgctg acgctggcac aaggcgatgc gacgctggcg
    14461 ttgcaactga ccgatgaaat gctgtcagga cgctttcagc cagccacgcc aacattcctc
    14521 aactgcggta agcagcagcg cggcgaactg gtttcctgtt ttctgctgcg tattgaagac
    14581 aatatggagt cgattggccg ggcggtaaat tccgcactgc aactgtcgaa acgcggcggc
    14641 ggcgtggcat ttttgctgtc gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa
    14701 aatcaatctt ctggcgtgat tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc
    14761 aaccagctcg gcgctcgtca gggggctggt gcagtctatt tacatgccca tcatcccgat
    14821 attctgcgtt ttctcgatac gaaacgggaa aatgccgacg agaaaatccg cattaaaaca
    14881 ctgtcgctgg gcgtggtgat cccggatatc actttccatc tggcaaaaga gaatgcgcag
    14941 atggcgctgt tttcgcctta tgacgtagag cgagtttatg gcaagccgtt tgccgatgtg
    15001 gccatcagcc aacactatga cgaactggtt gccgatgaac gcattcgcaa aaaatacctc
    15061 aacgcccgtg atttcttcca gcgactggca gaaatccagt ttgagtccgg ctatccctac
    15121 atcatgtatg aagacacggt aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt
    15181 aatctctgct cagaaatttt gcaggttaac agcgcctcag agtatgacga gaatctcgac
    15241 tatgcccgta caggccatga tatttcctgc aatttaggtt cgttgaatat tgcccacacc
    15301 atggattccc ccaactttgc ccgcacggta gagactgccg tacgcggttt aacggcggtg
    15361 tcagatatga gtcatatccg cagcgtgccg tccatcgaag ccggaaatgc cgcctcacac
    15421 gccatcgggc tggggcagat gaatttacac ggttatctgg cgcgagaagg catcgcttat
    15481 ggttcgccgg aagcactgga tttcaccaat ctctatttct ataccatcac ctggcacgca
    15541 ctgcgtacct cgatgttgct ggcacgcgaa cgcggtgaaa ccttcgccgg gttcaaacag
    15601 tcacgttatg ccagtggtga atatttcagc caatatctgc aaggtaactg gcagccgaaa
    15661 acggcgaaag ttggcgaact gtttgcccgc agcggtatta cgttacctac ccgtgagatg
    15721 tgggcgcaac tgcgcgacga cgtgatgcgc tacggcattt ataaccagaa tcttcaggcg
    15781 gtgccgccaa ccggttctat ctcttatatc aaccatgcta cgtcgagtat tcatccgatt
    15841 gtggcgaaag tagagatacg caaagagggc aaaacaggac gcgtttacta ccctgccccg
    15901 tttatgacta acgagaatct ggcgctgtat caggatgctt acgaaatcgg cgcagaaaag
    15961 atcatcgaca cctacgcgga agcgactcgc catgtcgatc agggtttgtc gctgacgctg
    16021 tttttccccg ataccgccac cactcgcgat atcaacaaag cgcagattta cgcctggcgc
    16081 aaaggtatca aaacgctcta ttacattcgc ctgcgtcaga tggcgctgga aggcactgaa
    16141 attgaaggct gcgtctcctg tgcactttaa ggaatatcta tgaaactctc acgtatcagc
    16201 gccatcaact ggaacaagat atctgacgat aaagatctgg aggtgtggaa tcgcctgacc
    16261 agcaatttct ggctaccaga aaaggtgccg ctgtcgaacg atattcctgc ctggcagaca
    16321 ttaactgtcg tagaacaaca actgacgatg cgcgttttta ctggcctgac gctgctcgac
    16381 acgctgcaaa atgttatcgg cgcgccttcg ctgatgcccg atgcactcac gcctcatgaa
    16441 gaagcggtat tatcgaatat cagctttatg gaagcggttc atgcccgctc ttacagttcg
    16501 attttctcga cgctgtgtca gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa
    16561 aacgcaccgt tgcagcgaaa agctcagatt attcagcaac attatcgcgg tgatgatccg
    16621 ctgaaaaaga aaatcgccag tgtgtttctt gaatcttttt tgttctattc cggtttctgg
    16681 ctgccgatgt atttttccag ccgcggaaag ctgaccaata ccgcagatct gatccgtctg
    16741 attatccgcg atgaagcagt ccacggttac tacataggct ataaatatca gaaaaacatg
    16801 gaaaagatat ctctgggaca acgtgaagag ttgaagagtt tcgccttcga tttattgctg
    16861 gaactctacg acaacgagtt gcaatacacc gatgagctgt acgccgaaac cccgtgggct
    16921 gacgatgtga aagcgtttct ctgttacaac gccaataagg ctttgatgaa tctgggctac
    16981 gaaccgttat ttcccgcaga aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg
    17041 ccgaatgccg atgaaaatca cgatttcttt tccggttcag gctcctctta tgtgatgggg
    17101 aaagcggttg aaacagaaga tgaagactgg aatttctgag ggtgttattt tcaaaaatat
    17161 cactacccgc agcagggaaa taattcccgc caaatagctt tttatcacgc aaatattctg
    17221 tagtgatcta cactgatact ctgttgcatt attcgcctga aactacaata ttcaggcgtt
    17281 ttttcgctat ctatgacaaa aaatatcaac ttttctcgat ttgctctcag cccttatatc
    17341 acgggaaatt ccggcgattt gctcgcatca atattcatgc cacatttgcc ctcaggggtt
    17401 gcctcagatt ctcagtatgt tagggtagaa aaaagtgact atttccattg ggtaatatat
    17461 cgacataaac aaataaagga atctttctat tgcatggcaa ttaaattaga aattaaaaat
    17521 ctttataaaa tatttggtga gcatccacag cgagcgttca aatatatcga acaaggactt
    17581 tcgaaagaac aaattctgga aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg
    17641 gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg taaatccaca
    17701 atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtgct gattgatggt
    17761 gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg tgcgcagaaa aaagattgcg
    17821 atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa tactgcgttc
    17881 ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct tgatgcactg
    17941 cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg atgaactctc tggcgggatg
    18001 cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatatt attaatggac
    18061 gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga gctggtaaaa
    18121 ttacaggcga aacatcagcg caccattgtc tttatttccc acgatctcga tgaagctatg
    18181 cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt cggcacaccg
    18241 gatgaaattc tcaataatcc ggcgaatgat tatgtccgta ccttcttccg tggcgttgat
    18301 attagtcagg tattcagtgc gaaagatatt gcccgccgga caccgaatgg cttaattcgt
    18361 aaaacccctg gcttcggccc acgttcggca ctgaaattat tgcaggatga agatcgcgaa
    18421 tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtctccat cgattcgctt
    18481 aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc gccgttagca
    18541 gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc accctgtgcg
    18601 gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg aatgctgctg
    18661 cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt gggataccac
    18721 gccagcggcg gacagtgccg cgcaatccgc agacgcctgg ggtacaccgg cgactgcacc
    18781 gactgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg tcgagcattt
    18841 taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg tcactgaagg
    18901 gatcgactgg gttgttaccc atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga
    18961 ttatatcctc aacggtttcc agcagttgct gctgggtatg cccgcaccgg tggcgattat
    19021 cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcgtgg cgacgctggt
    19081 ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag gcaatggtta ctctggcgct
    19141 ggtgttaacc gccctgctgt tctgtatcgt catcggtttg ccgttgggga tctggctggc
    19201 gagaagtccg cgagcggcga aaattattcg tccactgctt gatgccatgc agaccacgcc
    19261 agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg tgccgggcgt
    19321 ggtggtgacg atcatctttg ctctgccgcc gattatccgt ctgaccattc tggggattaa
    19381 ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat
    19441 gctgttcaaa gttcagttac cgctggcgat gccgaccatt atggcgggcg ttaaccagac
    19501 gctgatgctg gccctttcta tggtggtcat tgcctcgatg attgccgtcg gcgggctggg
    19561 tcagatggta cttcgcggta tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt
    19621 cgggattgtg atcctcgcca ttatcctcga tcgcctgacg caggccgttg ggcgcgactc
    19681 acgcagtcgc ggcaaccgtc gctggtacac cactggccct gttggtctgc tgacccgccc
    19741 attcattaag taactctgca cttgcccggt gacgccgggc attatcaccc tgccaaaaaa
    19801 aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc ttatctctac
    19861 acaaactttt gctgccgatc tgccgggcaa aggtattact gttaatccag ttcaaagcac
    19921 catcaccgaa gaaaccttcc agacgctgct ggtcagtcgt gcgctggaga aattaggtta
    19981 taccgtcaac aaacccagcg aagtggatta caacgttggc tacacctcgc ttgcttccgg
    20041 cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt acgaagctgc
    20101 cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg cacagggtta
    20161 cctgatcgat aagaaaaccg ccgaccagta caaaatcacc aacatcgcac aactgaaaga
    20221 tccgaagatc gccaaactgt tcgataccaa cggcgacgga aaagcggatt taaccggttg
    20281 taaccctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt atgaactgac
    20341 ccataccgtg acgcataatc aggggaacta cgcggcgatg atggccgaca ccatcagtcg
    20401 ctacaaagag ggcaaaccgg tgttttacta cacctggacg ccgtactggg tgagtaacga
    20461 actgaagccg ggcaaagatg tcgtctggtt gcaggtgccg ttctccgcac tgccgggcga
    20521 taaaaacgcc gataccaaac tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat
    20581 gcatatcgtt gccaacaaag cctgggccga gaaaaacccg gcagcagcga aactgtttgc
    20641 cattatgcag ttgccagtgg cagatattaa cgcccagaac gccattatgc atgacggcaa
    20701 agcctcagaa ggcgatattc agggccacgt tgatggctgg atcaaagccc accagcagca
    20761 gttcgatggc tgggtgaatg aggcgctggc agcgcagaag taatttttat tcaggcggat
    20821 aaggcgtttt tgcctcatcc gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc
    20881 ttatcatgcc tacaaaatac actcattccg tatggcggat aaggcgtttt cgccgcatcc
    20941 gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac
    21001 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
    21061 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
    21121 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtcgcca gtaactatta
    21181 cgctcagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
    21241 ctttattgtt accgccgcgc aattgggcta tgcagcaggt ctgctgtttc ttgttcccct
    21301 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cggcaggcgg
    21361 catgttaatc actgccagca gtcagtcgct ggcgatgatg atcctcggta cggcattaac
    21421 cggtttattc tctgtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
    21481 ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt ctgctgttgg ggatcttgct
    21541 ggcacggaca gttgccggat tgctggcgaa tctcggcggc tggcgcaccg tcttttgggt
    21601 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
    21661 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagta tgtttatcag
    21721 cgataaaatt ctgcgtaccc gcgcgttgct gggctgcctg acctttgcca atttcagcat
    21781 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtgt
    21841 aattggtctg tttggacttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
    21901 ttttgccgat aagggcaaat cgcaccacac cacaactttc ggtctgctgc tgctattact
    21961 ttcatggctg gcgatctggt ttggtcacac ttccgtactg gcgttgatta tcggaatcct
    22021 ggtgctggat ctcaccgtgc agggcgtgca tatcactaac cagacggtaa tttatcggat
    22081 acatcctgat gcgcgcaatc gcctgaccgc aggttacatg actagctact ttattggcgg
    22141 tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg
    22201 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
    22261 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
    22321 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
    22381 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
    22441 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata ttccggtggc
    22501 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
    22561 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg
    22621 gagtagtttg tggattgctg cactgaccgt catggcaatg gatgttcgcc atgtgttgta
    22681 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctgtg
    22741 ggcgtttggc ctgacggatg aggtttttgc cgccgcaacc gcaaaactgg tacgcaataa
    22801 tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
    22861 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
    22921 tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
    22981 ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac
    23041 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
    23101 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctactg cttgggttac
    23161 tggttggcgc ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg
    23221 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
    23281 tatgcgctct gctggttgtc tctaccgcac cagaagtaat gcacgataca cgccgtttcg
    23341 tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
    23401 tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta
    23461 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt caccgtcgtt
    23521 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
    23581 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
    23641 tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat aaaatgctga
    23701 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
    23761 accacagtat tcagccttct gaattaagtt gtgctcttgg gtcatcccgt accaacgcga
    23821 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg
    23881 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt
    23941 taccaccgca gcataactgc ctgcatctac tctggtccgc gctcagcaca acagaaaaag
    24001 atcagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg
    24061 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gacttataaa
    24121 agaaaaaatg actggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca
    24181 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
    24241 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
    24301 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcatttcg
    24361 aagaaaccga tgacgcatac gtggcaggga atcaaattca aattatgtct caggtgtctg
    24421 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gacgtgctgg
    24481 tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt
    24541 ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttgcag gcgaatattg
    24601 aggtgcagaa aatcgccctc gcaaaagcac aaagcgacta caaccgccgt gtgccgctgg
    24661 gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg
    24721 cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg atgattctgg
    24781 ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta cgtaacgcct
    24841 ggctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtc tcccgccgcg
    24901 cggtacagcc tggggcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
    24961 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgtatcggtc
    25021 agccggtcac tatcaccacg gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
    25081 ttggtctgga tatgggcaca ggtagcgcat tctcactgct tccggcgcaa aatgcgaccg
    25141 gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat cgaactggac cagaaacagc
    25201 tggagcaata cccgctgcgc attggtttgt ccacgctggt gagcgtcaat accactaacc
    25261 gtgacggtca ggtactggca aataaagtac gttcaacacc ggtcgcggta agtaccgcgc
    25321 gtgaaatcag cctagcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
    25381 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc
    25441 attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctggactc caccattgct
    25501 aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca gggaacgtgg
    25561 gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
    25621 aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc tattgcgtcg
    25681 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
    25741 attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa ctacccgcca
    25801 gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc
    25861 ggcccgatcc tcggcggtta tatcagcgat aattaccact ggggctggat attcttcatc
    25921 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaactctgcg cggacgtgaa
    25981 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
    26041 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actggttttc atcacaggaa
    26101 attatcattc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt ctgggagctg
    26161 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
    26221 tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg ctattgttct gctgccgcag
    26281 ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta
    26341 gggattattc cggtgatcct gtcgccgatt atcggccgct tcgcgcataa actggatatg
    26401 cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
    26461 acctttgaac caggtatgga ttttggcgcg tcggcctggc cgcagtttat ccaggggttt
    26521 gcggtggcct gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa
    26581 cgactggcgg cggcatcgag cctctccaac tttacgcgta cgctggcggg gtctatcggc
    26641 acgtccatca ccaccaccat gtggaccaac cgcgagtcga tgcaccacgc acagttaacc
    26701 gagtcggtaa acccgtttaa cccaaacgcc caggcgatgt acagtcaact ggaagggctt
    26761 gggatgacgc aacagcaggc ctccggctgg attgcccagc agatcaccaa ccaggggctg
    26821 attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt cctgctgggg
    26881 ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa
    26941 gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac
    27001 tgactaaatg tgcagttcct gcaacttctc ttccggcagc gccagttctt cgttgctgtt
    27061 gatgcgcacg tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg
    27121 catctggtaa gtgccacact ggtagacgtt cagttccggg atctgattct gatcctgcac
    27181 tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct catctggcgt
    27241 accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc
    27301 tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg
    27361 gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt
    27421 gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat
    27481 ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc cggtaatttt
    27541 tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat
    27601 ttgttatcat catccctgaa ttcagagatg aaattttggc cactcacgag tggccttttt
    27661 cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc agcggcttcc
    27721 atttcctgct gacggcgttc agacgcctcg cgctcggcta caaaatcctc ttcgcgcaga
    27781 atttccagcg gctcttcacg cagcagatta cggtaggctt ctgcaaatgc tttgcctgtt
    27841 ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga
    27901 ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaata
    27961 ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcggt
    28021 aacgggaact gtgcggtttc gcagccgata cccagcgtca gacccggttt gcgaccttcg
    28081 aggatcaccc ggttccagtt aacgcgtgta caggcaagtt cgctactgct catttccggt
    28141 gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct
    28201 acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg
    28261 ccacgtaaca gcgcatcaga aggcgactcg ccgctgcggg taacgcgttt tggacgaatc
    28321 ggcgcgtaca gttcgttttc aatctgcaac acgttgctgt tgatttgcag cctcttaccg
    28381 tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttaagg
    28441 cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg
    28501 gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac
    28561 tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga acaaatcgcc
    28621 ggagatgcac caaacagata aggaatgacc caaccgaaac gatagtaatt gcggataacg
    28681 cggaaatagc ccgcagaaat tttctctttg gcatcagcgc ccgagatatc accgcacttc
    28741 gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc
    28801 agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg tccggtgtta
    28861 gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt
    28921 aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata
    28981 aaggtcagca tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc
    29041 gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct
    29101 gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt
    29161 atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg
    29221 atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca
    29281 tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtcgc tgctgcaact
    29341 gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttaccggtcc ccacgagatg
    29401 cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat
    29461 agcgtgactg caccatagcg tttcagccag ccggtagctt tctcttgcca gcgcgatgtt
    29521 ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag gctattaccc
    29581 attgttgctg ttaaaactaa aacccaggga tgactgatcc cggaaagcaa cattgccacc
    29641 agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac
    29701 gagaaaagcg ataacgcttc actcacagca agcgaacatc cacggcgtcc atgcctgctg
    29761 cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca
    29821 cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt
    29881 gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct
    29941 cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt
    30001 tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt cttactgctt
    30061 ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg
    30121 cctgagcaat acgccaggtg ggcgatccat taagcgcaat cattgcctga acatcgtact
    30181 gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca
    30241 ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc
    30301 aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag
    30361 gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga
    30421 aatttttggg agaagatggt gcatccggga ggattactca ctggcgttcg cccttcgggc
    30481 cgttgctgac gcaacgttat cctccctgga gcctgcggtt aataccgcta tctcttcgat
    30541 accttcattg ctgaaggtt
//
LOCUS       DAHBHC010000061        30338 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHBHC010000061 DAHBHC010000000
VERSION     DAHBHC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.061"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..567
                     /gene="tuf"
                     /locus_tag="M2A35_003271"
     CDS             <1..567
                     /gene="tuf"
                     /locus_tag="M2A35_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCB7199530.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            797..1180
                     /gene="secE"
                     /locus_tag="M2A35_003272"
     CDS             797..1180
                     /gene="secE"
                     /locus_tag="M2A35_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCB7199531.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1182..1727
                     /gene="nusG"
                     /locus_tag="M2A35_003273"
     CDS             1182..1727
                     /gene="nusG"
                     /locus_tag="M2A35_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCB7199532.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1886..2314
                     /gene="rplK"
                     /locus_tag="M2A35_003274"
     CDS             1886..2314
                     /gene="rplK"
                     /locus_tag="M2A35_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCB7199533.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2318..3022
                     /gene="rplA"
                     /locus_tag="M2A35_003275"
     CDS             2318..3022
                     /gene="rplA"
                     /locus_tag="M2A35_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCB7199534.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3435..3932
                     /gene="rplJ"
                     /locus_tag="M2A35_003276"
     CDS             3435..3932
                     /gene="rplJ"
                     /locus_tag="M2A35_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCB7199535.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3999..4364
                     /gene="rplL"
                     /locus_tag="M2A35_003277"
     CDS             3999..4364
                     /gene="rplL"
                     /locus_tag="M2A35_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCB7199536.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4684..8712
                     /gene="rpoB"
                     /locus_tag="M2A35_003278"
     CDS             4684..8712
                     /gene="rpoB"
                     /locus_tag="M2A35_003278"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCB7199537.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8789..13012
                     /gene="rpoC"
                     /locus_tag="M2A35_003279"
     CDS             8789..13012
                     /gene="rpoC"
                     /locus_tag="M2A35_003279"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCB7199538.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13253..14386)
                     /gene="thiH"
                     /locus_tag="M2A35_003280"
     CDS             complement(13253..14386)
                     /gene="thiH"
                     /locus_tag="M2A35_003280"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCB7199539.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(14383..15153)
                     /gene="thiG"
                     /locus_tag="M2A35_003281"
     CDS             complement(14383..15153)
                     /gene="thiG"
                     /locus_tag="M2A35_003281"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCB7199540.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15155..15355)
                     /gene="thiS"
                     /locus_tag="M2A35_003282"
     CDS             complement(15155..15355)
                     /gene="thiS"
                     /locus_tag="M2A35_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCB7199541.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15339..16094)
                     /gene="thiF"
                     /locus_tag="M2A35_003283"
     CDS             complement(15339..16094)
                     /gene="thiF"
                     /locus_tag="M2A35_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCB7199542.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16087..16722)
                     /gene="thiE"
                     /locus_tag="M2A35_003284"
     CDS             complement(16087..16722)
                     /gene="thiE"
                     /locus_tag="M2A35_003284"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCB7199543.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16722..18617)
                     /gene="thiC"
                     /locus_tag="M2A35_003285"
     CDS             complement(16722..18617)
                     /gene="thiC"
                     /locus_tag="M2A35_003285"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCB7199544.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18698..18798)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18850..19326)
                     /gene="rsd"
                     /locus_tag="M2A35_003286"
     CDS             complement(18850..19326)
                     /gene="rsd"
                     /locus_tag="M2A35_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCB7199545.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19421..20194
                     /gene="nudC"
                     /locus_tag="M2A35_003287"
     CDS             19421..20194
                     /gene="nudC"
                     /locus_tag="M2A35_003287"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCB7199546.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20234..21298
                     /gene="hemE"
                     /locus_tag="M2A35_003288"
     CDS             20234..21298
                     /gene="hemE"
                     /locus_tag="M2A35_003288"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCB7199547.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21308..21979
                     /gene="nfi"
                     /locus_tag="M2A35_003289"
     CDS             21308..21979
                     /gene="nfi"
                     /locus_tag="M2A35_003289"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCB7199548.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WGWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22022..22612
                     /locus_tag="M2A35_003290"
     CDS             22022..22612
                     /locus_tag="M2A35_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCB7199549.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22799..23071
                     /gene="hupA"
                     /locus_tag="M2A35_003291"
     CDS             22799..23071
                     /gene="hupA"
                     /locus_tag="M2A35_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCB7199550.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23084..23779
                     /locus_tag="M2A35_003292"
     CDS             23084..23779
                     /locus_tag="M2A35_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCB7199551.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23781..24206)
                     /gene="zraP"
                     /locus_tag="M2A35_003293"
     CDS             complement(23781..24206)
                     /gene="zraP"
                     /locus_tag="M2A35_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCB7199552.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            24444..25820
                     /gene="zraS"
                     /locus_tag="M2A35_003294"
     CDS             24444..25820
                     /gene="zraS"
                     /locus_tag="M2A35_003294"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCB7199553.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25817..27142
                     /gene="zraR"
                     /locus_tag="M2A35_003295"
     CDS             25817..27142
                     /gene="zraR"
                     /locus_tag="M2A35_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCB7199554.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27139..28428)
                     /gene="purD"
                     /locus_tag="M2A35_003296"
     CDS             complement(27139..28428)
                     /gene="purD"
                     /locus_tag="M2A35_003296"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCB7199555.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28440..30029)
                     /gene="purH"
                     /locus_tag="M2A35_003297"
     CDS             complement(28440..30029)
                     /gene="purH"
                     /locus_tag="M2A35_003297"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCB7199556.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7107 a         7891 c         8359 g         6981 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggtcgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      541 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
      601 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      661 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      721 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaaaact
      781 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
      841 agcgatgaag tgggtcgttg tggtggcatt gctcctggtg gcgattgtcg gcaactacct
      901 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
      961 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc gttgcttttg cccgtgaagc
     1021 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acacaacgct
     1081 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
     1141 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
     1201 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgtcgctgc
     1261 gtgagcatat caaattacac aacatggaag atttgtttgg tgaagtcatg gtaccaaccg
     1321 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
     1381 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacctggtg cgcagcgtac
     1441 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
     1501 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgc
     1561 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggtg
     1621 ttgttgaaga agtggattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1681 gtgcgacccc ggtagagctg gacttcagcc aggttgaaaa agcctaaccc agcgatcaaa
     1741 aaagcggcga tttaatcgtt gcacaaggcg tgagattgga atacaatttc gcgccttttg
     1801 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     1861 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     1921 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     1981 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     2041 tctgccgatt ccggtagtaa tcaccgttta cgctgaccgt tctttcactt tcgttaccaa
     2101 gaccccgccg gcagcagttc tgctgaaaaa agcggctggt atcaagtctg gttccggtaa
     2161 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     2221 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     2281 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     2341 gcgtgttatc cgcgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     2401 actgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     2461 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     2521 tactggccgt tccgttcgcg tagccgtatt tacccaaggt gcaaacgctg aagctgctaa
     2581 agctgcaggc gcagaactgg taggtatgga agatctggct gaccagatca agaaaggcga
     2641 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2701 ccaggttctg ggtccgcgcg gcctgatgcc aaacccgaaa gtgggtactg taacaccgaa
     2761 cgttgctgaa gcggttaaaa acgctaaagc tggccaggtt cgttaccgta acgacaaaaa
     2821 cggcatcatc cacaccacca tcggtaaagt ggactttgac gctgacaaac tgaaagaaaa
     2881 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     2941 catcaagaaa gttagcatct ccaccaccat gggtgcaggt gttgcagttg accaggctgg
     3001 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     3061 ccccacgtat aatgcttaat gcagacgtat atccgagata ttcgggttgt ggcaaggcgg
     3121 caactgagtg agtcgccagg agcatagcta actatgtgac tggtgcgaat gaaggaagcc
     3181 aacgccgcca caagttgaat agcgacggat agaaaagatt tgttcgttgg agcctggcct
     3241 atccaggcct ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac
     3301 ggtgacagaa cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta
     3361 agacgctctc tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc
     3421 caggagcaaa gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca
     3481 gcgaagtagc caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag
     3541 ataaaatgac tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc
     3601 gtaacaccct gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt
     3661 ttgttggtcc gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt
     3721 tcaaagagtt cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg
     3781 agctgatccc ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa
     3841 ttgcacgcct gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg
     3901 ctgctgtacg cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcattcgct
     3961 tacgtataaa cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa
     4021 tcattgaagc agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg
     4081 aagaaaaatt cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag
     4141 ctgctgaaga aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg
     4201 ctgttatcaa agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg
     4261 tagaatctgc accggctgct ctgaaagaag gcgtgagcaa agacgacgca gaagcactga
     4321 aaaaagctct ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt
     4381 gcagcctgag aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg
     4441 taaggcgcca gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc
     4501 tatcgacgac ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg
     4561 tttaagcgtg atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag
     4621 tgttgcacaa actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac
     4681 cctatggttt actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca
     4741 caagttctgg atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc
     4801 gagcaagatc ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt
     4861 cagagctaca gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg
     4921 tttgacgtcc aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa
     4981 ctgcgtctgg tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa
     5041 caagaagtct acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac
     5101 ggtactgagc gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc
     5161 gacaaaggta aaacccactc ttcgggtaaa gtgctgtata acgcgcgtat catcccttac
     5221 cgtggttcct ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac
     5281 cgtcgccgta aactgcctgc gaccatcatt ctgcgcgccc tgaactacac cacagagcag
     5341 atcctcgacc tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg
     5401 gaactggtgc cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt
     5461 aaagtgtacg tagaaaaagg ccgccgtatc actgcgcgcc acattcgcca gctggaaaaa
     5521 gacgacgtca aactgatcga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa
     5581 gactatattg atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg
     5641 gatctgctgg ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac
     5701 gatctggatc acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt
     5761 ctgagcgcac tggtagaaat ctaccgcatg atgcgccctg gcgagccgcc gactcgtgaa
     5821 gcagctgaaa gcctgttcga gaacctgttc ttctccgaag accgttatga cttgtctgcg
     5881 gttggtcgta tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatc
     5941 ctgagcaaag acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa
     6001 ggcgaagtcg atgatatcga ccacctcggc aaccgtcgta tccgttccgt tggcgaaatg
     6061 gcggaaaacc agttccgcgt tggcctggta cgtgtagagc gtgcggtgaa agagcgtctg
     6121 tctctgggcg atctggatac cctgatgcct caggatatga tcaacgccaa gccgatttcc
     6181 gcagcagtga aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac
     6241 ccgctgtctg agattacgca caaacgtcgt atctccgcac tcggcccagg cggtctgacc
     6301 cgtgaacgtg caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt
     6361 ccaatcgaaa cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca
     6421 cagactaacg aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta
     6481 actgacgaaa ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg
     6541 aactccaacc tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa
     6601 ggcgaatcca gcttgttcag ccgcgaccag gttgactaca tggacgtatc cacccagcag
     6661 gtggtatccg tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca
     6721 ttgatgggtg cgaacatgca acgtcaggcc gttccgactc tgcgcgctga taagccgcta
     6781 gttggtactg gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggtagctaaa
     6841 cgtggtggtg tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac
     6901 gagatgtatc cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct
     6961 aaccagaaca cctgtatcaa ccagatgccg tgtgtgtctc tgggtgaacc ggttgaacgt
     7021 ggcgacgtgc tggcagacgg tccgtccacc gacctcggtg aactggcgct tggtcagaac
     7081 atgcgcgtag cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc
     7141 gagcgtgttg ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg
     7201 tcccgtgaca ccaagctggg gccggaagag atcaccgctg acatcccgaa cgtgggtgaa
     7261 gctgcgctct ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt
     7321 ggcgacattc tggttggtaa ggtaacgccg aaaggtgaaa ctcagctgac cccagaagaa
     7381 aaactgctgc gtgcgatctt cggtgagaaa gcctctgacg ttaaagactc ttctctgcgc
     7441 gtaccaaacg gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta
     7501 gaaaaagaca aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac
     7561 ctgtctgaag aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg
     7621 gtagccggtg gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag
     7681 ctaggcctga cagacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac
     7741 gaactgaaac acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc
     7801 gacgatctgg caccgggcgt gctgaagatt gttaaggtat atctggcggt taaacgccgt
     7861 atccagcctg gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc
     7921 aacccgatcg aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac
     7981 ccgctgggcg taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg
     8041 gctgcgaaag gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg
     8101 aaactgcgcg aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt
     8161 gacctgagta ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt
     8221 atgccaatcg caacgccggt gttcgacggt gcgaaagaag cagaaattaa agagctgctg
     8281 aaacttggcg acctgccgac ttccggtcag atccgcctgt acgatggtcg cactggtgaa
     8341 cagttcgagc gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc
     8401 gacgacaaga tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg
     8461 ggtggtaagg cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa
     8521 gcatacggcg cagcatacac cctgcaggaa atgctcaccg ttaagtctga tgacgtgaac
     8581 ggtcgtacca agatgtataa aaacatcgtg gacggcaacc atcagatgga gccgggcatg
     8641 ccagaatcct tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg
     8701 gaagacgagt aattctcgct caaacaggtc actgctgtcg gggtaaaacc cggcagcgga
     8761 ttgtgctaac tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca
     8821 gactaaaacc gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg
     8881 ttcatggtct ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc
     8941 agaacgtgac ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct
     9001 gtgcggtaag tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggcgttga
     9061 agtgacccag actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac
     9121 tgcgcacatc tggttcctga aatcgctgcc gtcccgtatc ggtctgctgc tcgatatgcc
     9181 gctgcgcgat atcgaacgcg tactgtactt tgaatcctat gtggttatcg aaggcggtat
     9241 gaccaacctg gaacgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga
     9301 gttcggtgac gaattcgacg cgaagatggg tgcggaagca atccaggctc tgctgaagag
     9361 catggatctg gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga
     9421 aaccaagcgt aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg
     9481 taacaaacca gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc
     9541 gctggttccg ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg
     9601 cgtcattaac cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat
     9661 cgtacgtaac gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg
     9721 tcgcggtcgt gcgatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat
     9781 caaaggtaaa cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg
     9841 tcgttctgta atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa
     9901 aatggcgctg gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc
     9961 taccaccatt aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat
    10021 cctggacgaa gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg
    10081 tctgggtatc caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc
    10141 gctggtttgt gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc
    10201 gctgacgctg gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct
    10261 gtccccggcg aacggcgaac caatcatcgt tccgtctcag gacgttgttc tgggtctgta
    10321 ctatatgacc cgtgattgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa
    10381 agaagcagaa cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg
    10441 tatcaccgag tatgaaaaag atgcaaacgg tgaattagta gcgaaaacca gcctgaaaga
    10501 cacgactgtt ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttactccat
    10561 cgtcaaccag gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat
    10621 tctcggtctg aaaccgaccg ttatttttgc ggaccagatc atgtacaccg gcttcgccta
    10681 tgcagcgcgt tctggtgcat ctgttggtat cgatgacatg gtcatcccgg agaagaaaca
    10741 cgaaatcatc tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg
    10801 tctggtaact gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga
    10861 tcgtgtatcc aaagcgatga tggataacct gcaaactgaa accgtgatta accgtgacgg
    10921 tcaggaagag aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg
    10981 tggttctgcg gcacagattc gtcagcttgc tggtatgcgt ggtctgatgg cgaagccgga
    11041 tggctccatc atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca
    11101 gtacttcatc tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaactgc
    11161 gaactccggt tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga
    11221 agacgattgt ggtacccatg aaggtatcat gatgactccg gttatcgagg gtggtgacgt
    11281 taaagagccg ctgcgcgatc gcgtactggg tcgtgtaact gctgaagacg ttctgaagcc
    11341 gggtactgct gatatcctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct
    11401 gctggaagag aactctgtcg acgcggtaaa agtacgttct gttgtatctt gtgacaccga
    11461 ctttggtgta tgtgcgcact gctacggtcg tgacctggcg cgtggccaca tcatcaacaa
    11521 aggtgaagca atcggtgtta tcgcggcaca gtccatcggt gaaccgggta cacagctgac
    11581 catgcgtacg ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca
    11641 ggtgaaaaac aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg
    11701 taaactggtt atcacttccc gtaacaccga actgaaactg atcgacgaat tcggtcgtac
    11761 caaagaaagc tacaaagtgc cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt
    11821 tgctggcggc gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt
    11881 aagcggtttt gtacgcttta ctgacatgat cgacggccag accattactc gtcagaccga
    11941 cgaactgacc ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg
    12001 taaagatctg cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc
    12061 tggtaccgat atgcctgcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga
    12121 tggcgtacag atcagctctg gtgacaccct ggcgcgtatt ccgcaggaat ccggcggtac
    12181 caaggacatc accggtggtc tgccacgcgt tgcggacctg ttcgaagcac gtcgtccgaa
    12241 agagccggca atccttgctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg
    12301 taaacgtcgt ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc
    12361 gaaatggcgt cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc
    12421 cgacggtccg gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgtgactcg
    12481 ttacatcgtt aacgaagtac aggacgtata ccgtctgcag ggcgttaaga ttaacgataa
    12541 acacatcgaa gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggcag
    12601 ctccgacttc ctggaaggcg aacaggttga atactctcgc gtcaagatcg caaaccgcga
    12661 actggaagcg aacggcaaag tgggtgcaac ttactcccgc gatctgctgg gtatcaccaa
    12721 agcgtctctg gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt
    12781 gctgaccgaa gcagccgttg cgggcaaacg cgacgaactg cgcggcctga aagagaacgt
    12841 tatcgtgggt cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg
    12901 tcgccgtgct gcgggtgaag ctccggctgc accgcaggtg actgccgaag acgcatctgc
    12961 cagcctggca gaactgctga acgcaggtct gggcggttct gataacgagt aatcgttaat
    13021 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tgggtgttat tcagagggca
    13081 aattcttcac ggaattttta agaaaggcag ggcgaaacga ggaagaagct tttcgccctg
    13141 gacaacattc ctgctgacgg aactaccata aaaaaacaga acgctttcaa caaccttgcg
    13201 tttttcatct tccgtagcgg ctcggaataa atttacgttg ttacattctg tctcatggcc
    13261 tttgcgaggc gtgtcccaga tagctgtccc agtctttcca taccggctgc aaaccctgag
    13321 cggttaacgc ggccgcaact gcttctggtc tgcgatcgtc gtgcggtgag aactgttcca
    13381 gctcggggtg attgtcggca tagccacctg gctgcgtttt cgaaaaagcg ctgacgttat
    13441 taattgccag cggaatcacg cgatcgcgaa accacggtga ttcccgcgtg gagagtgaga
    13501 gttcaatctc cggtgcaagc agtcggaagg cgcagatggt ttgcactaac tggcgttcat
    13561 ccataatcga cgcaggctca atgccgccag tacacgggcg caggcgcgga aaggagacag
    13621 agtaacggct ttgccagtaa tgctgttgca gccatagcaa atgttcggca accatatagc
    13681 agtcaacgcg ccagttgtcg gaaaggccaa ttagcgcgcc gaggcctatc ttatcaatcc
    13741 ccgcacgccc cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt tttttgcctt
    13801 tcaaatgatg gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc atcacgccat
    13861 ccagaccaag ctgctttaac tcggcgtatt ccgtctccgc cagcggttgc acttccattt
    13921 gtagtgaaga gaactgttca cgcagggcgg ggagatgacg acgaaagtaa tccatcccca
    13981 ctttcgcctg atgttcacca gtgactaata gcagatgttc aaagcccatc tcccgtatag
    14041 cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgtttgatg cgattactca
    14101 tggaaaatcc gcagtacgtg cagtcgttag cgcaaagatt ggaaagataa agcgggacgt
    14161 agaaactaac tgtgttgcca aatcgctgac gggtcagacg ctgcgcccgt tgggccagtt
    14221 gttccagata gccactggcg gctggcgata acagcgccat catatcgtcg cgggtgagtt
    14281 gcgaggcatt tagcgcccgt tctacgtcag cagccgtttt gccgttgata cgcaggcgga
    14341 tgtcgtccca gtccagttgt cgccagcgat cgctgaaggt tttcatgccg atgcctccag
    14401 aaatccagtc agcgggctgg tggcatgagc aaaatgactg cggctgcccg gtccggactg
    14461 acgtgccagc aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt tgacgggatc
    14521 gtccgcgacg gcaattgccg tattcactaa caccgcgtcg gcccccattt ccagcgcctg
    14581 cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggcactg tggcctgctg
    14641 gataataatc tccagcatgg cgcgggtttc cagtccctga ttcgagccaa tcggcgcgcc
    14701 gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata ccggatcggc
    14761 cccgcagtaa ggcagcacga caaatccctg ttgtaccagc gtttcggcgg ctttcagggt
    14821 ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctctaatt ttaaccagtt
    14881 tgtgcctaac gcttcacgag ccagatgggc ggcgaaaatg gcttcttccg ctgttttcgc
    14941 cccggatgta tttggcagca gggtcacacc cgccgcgata agcggttcga ggatagcgtc
    15001 gttgtgctgg cgcaagtcga cacgtttcat cgccagtgtc accagctggc tgccggaagc
    15061 gcggatcgcc tccaccatca gttgtgaaga agcgaatttc cctgtgccgg taaacagatg
    15121 tgaatcaaac gttttgtccg caatacgtaa catttcaacc ccctgcaata acctgaaaaa
    15181 gcaggatctg gtcgccatcc tgcacgatat gttgcgccca ctgctcacgc gggacgattt
    15241 gctgattaat cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc agtagttcgt
    15301 gaacagtttg cccggcggcg cactgcatcg gttgatcgtt aaacaggatc tgcattgctt
    15361 cctccgcata ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca ctggctcgat
    15421 ttaccgtcga acagtcggag ttctcccgca ggtgtctcta taccgcttaa taacttaatg
    15481 gcttccagtg cctgcaaagt gcccataacc ccgaccaccg ggccaaccac gcccgccgtg
    15541 cggcagttgc gttctggctc ctggttatct ggccacaggc agcggtaaca cccctgctcc
    15601 cagggcggcg tcagtaccat caactgaccg ccaaatccga ccgcgctggc ggtgataagc
    15661 ggcgtgttga gtgccacgca ggcggcatta atctcctggc gagtcgccat attgtcggta
    15721 cagtcgagca ccacatcggc ccgtgcaacc gcatctttta acgcctcacc cgttaaccgt
    15781 tgttgtaatg ctgtcagttg aatgtcggga ttcaactgtg tcagtcgctg ttggctgacc
    15841 tgcgatttcg ggctgttgat atcttcagtg gtaaagagga tttgtcgttg cagattgctt
    15901 aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcgcc cgccaggtac
    15961 agcgcagcag gtgtacccag cccgcccaga ccgataatca gcacctggct gtcgagcagt
    16021 ttttgctgcc cgtccagagc gatatcgtcg agcaggattt ggcggctata acgcataaag
    16081 tcacggtcat tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg ccaaacgcca
    16141 gtctgcagct tgagtaatgg cgctgacgac ggcgatactg ccgacacccg ttgctatcac
    16201 cgcaggcgcg cgtgccagac tgataccgcc aatcgccacg gtgggataat ccgccagtcg
    16261 ctcaacatgc cgtgccagct gttccagccc ctgcggtgca gaaggcatct gtttggtttg
    16321 cgtcgggaac acatgtccca gcgcgatata agaggggcgt gctgccagcg cgacgtcgat
    16381 ttccatatcg tcatgtgtcg aaacgcccag ccgcaggcct gccgcgcgga tggcattgag
    16441 atcggtggct tgcaaatctt cctgccccaa atggacgcca tacgcctgat gcttgatcgc
    16501 cagccgccag taatcgttga taaacaatcg cgcgttatag cggcggccca gcgcaattgc
    16561 cgccacgaca tcggcttcca cctcttcatc gcgccgatct ttgatgcgta gctggagagt
    16621 acgtacgcct gcatccaaca gacgttcgat ccactgtacg ctgtccacca ccgggtacag
    16681 tcctaaacga aaaggtacag gaggaaaatc aggctgatac atcacgcttc ctccttacgc
    16741 aggtagattt ctccgcctct ggcacggaag ttctccgaca tatccgccat tcccatttca
    16801 atagtttgcg tggcggcgta atcacgcact tcctggctga ttttcatcga acagaatttc
    16861 ggcccacaca tggagcaaaa atgggcgact ttacctgact cttgcggcag ggtttcatcg
    16921 tgataagcgc gggcggtaaa cgggtcgagg gccagattaa actggtcttc ccagcgaaat
    16981 tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg ccctttcgcc
    17041 aggtcagcgg cgtgggcggc aatcttatag gtgataagcc cctgcttaac atcttcttta
    17101 ttaggcagac ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca gccaaaccag
    17161 ccaatcatcg ccgcaccaat ccccgacgtg aagtggtcat agcccggcgc aatatcggta
    17221 gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc ctcggtcata
    17281 ttgcggcgga tcatctgcat cggcacgtga cctgggcctt caatcatcac ctgcacgtca
    17341 tattcccagg caattttggt cagttcgccc agcgtatgca gctcggcaaa ctgcgcttca
    17401 tcgttggcgt cctgaataga accggggcgc agaccgtcgc ccagcgacag cgaaacgtca
    17461 taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt ttcctgatga
    17521 tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc ggtcaggcgt
    17581 ttcgcggtca tcggcacata gcgcagcagt acgcccgcat ggatagtgaa gtaatccaca
    17641 ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag atcttcggcg
    17701 atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg caccgggctg
    17761 ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag atccatcacc
    17821 gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc gatggaagag
    17881 gtgaccgccg agttgccgat attggcgtta acttttacca ggaaattgcg accaataatc
    17941 atcggctccg attccggatg attaatgttg gccgggataa tcgcacgtcc ggcagcaact
    18001 tcatcacgga caaattccgc agtgatattt tccggcagat gtgcgccaaa gctcattccc
    18061 ggatgctggt ggcgtaaaac ctcgctacgg atgcgctcgc ggcccatatt ctcgcggatg
    18121 gcgatgaatt ccatttccgg cgtgatgatg ccctggcggg cgtagtgcag ttgggtgaca
    18181 cggcgtcctg ctttggcgcg ttttggtgtt agtacaccgc taaaacgcag ttcgtcgagg
    18241 ccatcatctg ccagccgcgc tttagtgtaa tcggaactgc gcacggtaag ttcttcggta
    18301 tcgccgcgcg catcgatcca cggctggcgt agttttgcca gcccttgctg cacgttaatg
    18361 gcaatctgcg gatcaccata cgggccggag gtgtcgtaga ccggaatcgc ttcgttttct
    18421 tcgtactgcg gctgttcttt gctaccgcca attagcgtcg ggctaagctg gatctcacgc
    18481 atcggcacgc gcacgccggg gtgtgtgcca gtgatataaa tgcgttttga gttgggaaag
    18541 gcggtgcctt ccagggtgtc gataaaatgt tgggcccggg cgcgttgttc gcggcgggtc
    18601 agttttgttg cagacatagc tcattccaaa aagttaagga cgtggcttgt cagacgacgg
    18661 atgaagcaag agacgatcgc cgcaggggcg atgcgatagc agattaactc ttgttccctt
    18721 cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca gccagttaag
    18781 gcactccgac aagaaattag ccccgcaaat ggggcattga atgtaaatta cgcgttaaca
    18841 gcgcagaact caagcaggat gtttgacgcg ggcggcgtca agcaccagca gaatcagctt
    18901 atcttccagc acaaagcgcg cttccagcgc ctcgccgatg tcagataaaa cctgttgaaa
    18961 ctcaaggtaa ttatcatgat cgatggcggt ttccagactg gaatcgtagt aatccataat
    19021 ctgttgagta ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt
    19081 gccttccagc ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc
    19141 gaccaggctc tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt
    19201 gccaggctta atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag
    19261 ccagcgatca accagtttgt tacttcctct gacgcgctcc gtcaggttat cgagctggtt
    19321 aagcatgatt gactccgcaa gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc
    19381 atgttaaaca tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa
    19441 attagatcac ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga
    19501 attgccatat ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg
    19561 cgaatggcag ggggaacctg tttggttagt acaacagcag cggcgtcacg atatggggtc
    19621 ggtacgtcag gtcattgatc tcgatgttgg gctgtttcaa ctggccggac gaggcgtaca
    19681 actggcggag ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc
    19741 gagcaaaacc gaatgggcga tgctgtgcag ccattgccgt gagcgttact acccgcaaat
    19801 cgccccctgc attattgttg ccatccgtcg cgatgattcg atcctcctcg cccagcatac
    19861 ccgccatcgt aacggtgtcc atacagtact tgccggattc gtcgaagtgg gcgaaaccct
    19921 cgagcaggca gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg
    19981 ttacgtgact tctcagccgt ggccgtttcc tcagtcttta atgaccgcgt ttatggcgga
    20041 atatgacagc ggcgacatcg tgatcgaccc gaaagaattg ctcgaggcga actggtatcg
    20101 ctatgacgat ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga
    20161 tacggtggcg atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta
    20221 aggaacagcc aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca
    20281 gcccgttgat gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata
    20341 taaagccacg cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaatg ccgagctggc
    20401 gtgcgaagtg actttgcaac cgctgcgtcg ctacccgctg gatgcggcga tcctcttttc
    20461 cgatatcctc accgtgccgg acgcgatggg gttagggctc tattttgaag ccggagaagg
    20521 tccgcgtttt acctcgccag tcacctgcaa agccgacgtc gataaactgc caattccgga
    20581 cccggaagat gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa
    20641 aggcgaagtg ccgctgattg gtttttccgg cagcccgtgg acgctggcaa cctacatggt
    20701 ggaaggcggc agcagcaaag ccttcaccgt gatcaaaaaa atgatgtatg ccgatccgca
    20761 ggcgctgcac gctctactcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca
    20821 gattaaagcc ggtgctcagg ctgtgatgat tttcgacacc tggggcggcg tgcttaccgg
    20881 gcgcgattat caacagttct cgctttatta catgcataaa attgttgatg gtttactgcg
    20941 tgaaaacgac ggtcgccgcg taccggtcac gctgtttacc aaaggcggcg gacagtggct
    21001 ggaagccatg gcagaaaccg gttgcgatgc gctgggcctc gactggacaa cggacatcgc
    21061 cgatgcgcgc cgccgtgtgg gcaataaagt cgcgttgcag ggtaatatgg atccgtcgat
    21121 gctgtacgcg ccgcctgccc gtattgaaga agaagtagcg actatacttg caggtttcgg
    21181 tcacggcgaa ggtcatgtct ttaaccttgg tcacggcatt catcaggatg tgccgccaga
    21241 acatgctggc gtgttcgtgg aggcagtgca tcgactgtct gaacagtatc accgctaagg
    21301 agtgattatg gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat
    21361 ccgcgaggat cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt
    21421 tgagcagggc ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga
    21481 gctggtggag tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct
    21541 ttccttccgc gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga
    21601 tttagtgttt gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca
    21661 ttttggctta ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa
    21721 attcgaaccg ctctccagcg aaccgggcgc gctggcccca ctgatggata aaggcgagca
    21781 gctggcctgg ggctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca
    21841 tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct
    21901 gccggagcca acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta
    21961 tacagcaaat cagccctaat tcaggtaaac tgcgggtaat ttccgtattt gagaactcaa
    22021 catgttacaa aacccaattc atttgcgtct ggagcgcctg gaaagctggc agcacgtcac
    22081 tttcatggct tgcttatgcg aacgcatgta ccccaattac gccatgtttt gccagcaaac
    22141 cggttttggt gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac
    22201 cgttaaagac gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc
    22261 ttcagccgac gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt
    22321 aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag
    22381 taagacctcc atcacgaccg ttgcgatgct ggaaatgact caggctggtc gtgaaatgag
    22441 cgatgaagag cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat
    22501 attccgactt ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc
    22561 agacctgcgt gaggcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa
    22621 aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt
    22681 ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc
    22741 tggcatttcc gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat
    22801 gaacaagact caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc
    22861 taaagctgct ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc
    22921 tgtacaactg gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg
    22981 caacccgcag accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc
    23041 tggcaaggca ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga
    23101 aggggtggtt tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggcgctct
    23161 gtttctcact gcctgtagtc acaactcttc acttcctccc tttaccgcca gtggatttgc
    23221 tgaagaccag ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct
    23281 tgccgtgttt agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg
    23341 cgataacctc acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc
    23401 gcgttttgac gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca
    23461 gcagctttct aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat
    23521 tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga
    23581 acagaccgtg accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat
    23641 agcgcatatc gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga
    23701 agcgcccgaa ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc
    23761 caacgagaaa acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag
    23821 ccgccgtaac ccatgcccat tccggtaccg cgcggaatac ccgcttcagc catcgcaata
    23881 tctcgtttca cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc
    23941 gcgttaattt tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc
    24001 ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc
    24061 tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt
    24121 ccgccgtgag caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc
    24181 agggcaattt tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta
    24241 ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg
    24301 agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc
    24361 tttgtgttac agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa
    24421 gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta
    24481 aatggttaag cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga
    24541 cggtgattcg cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag
    24601 gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc
    24661 atcatgcgca gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt
    24721 ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa
    24781 aacagcttta ttccccgcag gaaatgcagc agttacatcc tggagatgaa gaagcgtggc
    24841 ggcggatcga tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga
    24901 tgtttgctgc tggaatgcac cggatgcgcc atatgcagca gtatgccgca acaccacaag
    24961 caattttcat cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa
    25021 acaccctgat tatcctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct
    25081 tctggtatcg ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag
    25141 agaagctggt ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac
    25201 tttcctcgat taaaggactg gcgaaatact ttgccgaacg cgcgccagca gggggagaag
    25261 cgcatcaatt ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg
    25321 agttgctgga actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc
    25381 tgattaacca ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac
    25441 gctttaccgc caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg
    25501 tcctgttgaa tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg
    25561 tgacggtcag cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa
    25621 ttgcggcaga tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca
    25681 ccggattggg gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg
    25741 tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc
    25801 gtaaggaccc acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat
    25861 tagccactgc actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc
    25921 gaacagcggg cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg
    25981 cgatgtgcga atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa
    26041 cccggcaatt ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc
    26101 actgaaaact ggggcgctgg attatctcat caagccgctg gatttcgata acctacaggc
    26161 gacgctggaa aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac
    26221 tgccagccag ttcggtatgg tcggtaaaag ctcggcgatg caacacctgc tcagtgaaat
    26281 cgccctcgtc gcgccatcgg aagccacggt actgatccac ggcgattccg gcaccggtaa
    26341 agagctggtc gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac
    26401 gctcaactgt gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa
    26461 aggggcgttt actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg
    26521 cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg
    26581 tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt
    26641 ccggctgatt gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg
    26701 ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg
    26761 gcgggaagat atccctctgc tggctggcca ttttctgcag cgctttgccg agcgtaatcg
    26821 caaggcggta aaaggtttta cgccccaggc gatggatctg ttgattcatt acgactggcc
    26881 gggaaatatt cgtgagctgg aaaacgcggt ggaacgggca gtggtgctgc tgaccgggga
    26941 atatatttcc gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca
    27001 aagtcaggat attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcactgga
    27061 gaaaacgggc ggcaacaaaa ctgaagccgc ccgtcagtta gggatcacgc gtaaaacgtt
    27121 attggcaaaa ctgtcgcgtt agttctgctc gcgttcgata gcacgccagc cgatatcttt
    27181 ccggcagaag cagtcgtccc agtggatatc ggtcattaag gcataggcgc gtttctgcgc
    27241 ttctgccacg gtatgaccca gcgcggtgac gcacagtacg cgtccgccgt tggttaccac
    27301 ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc
    27361 cagcggcagg ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc
    27421 catcaccacg ccgagagaag cgcgttcatc ccactcggac gttttctcgt ccagcttgcc
    27481 ttcacaggcc gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg
    27541 ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc
    27601 gatcatcagg cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt
    27661 cacggttggc cagataatac gttccatggt gcgctgatga acgtcatcgg ttactaccgg
    27721 cgcgggggag taagcgccca tcccgccggt gtttggtccg gtatctttat cgcctacgcg
    27781 tttgtgatcc tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa
    27841 gctcgcttct tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa
    27901 agcgttgccc gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac
    27961 gataacgcct ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc
    28021 acgcagatac gccagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat
    28081 cttatggcgg gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc
    28141 tgcggttggg ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac
    28201 cagcggcgct tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa
    28261 cagcgccggg atatcggtca cgccaatagc aacgttttgc agcgcgggtt ccagcgcagt
    28321 gcctgcatta cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca
    28381 ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat
    28441 taatggcgga agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg
    28501 gcaatcactt cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca
    28561 gcggcggcgg catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa
    28621 cctttcactt ccaggccttc atcagcagct ttaataccgg cgattttcgc ggagtacacg
    28681 cggctcatct ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca
    28741 ttggatttca cgaacttcgc cactttccag cagaacagcg catcgcgcag ttcctgttcg
    28801 gtcggctgac gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga
    28861 tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc
    28921 cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct
    28981 tcgctggcgg aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg
    29041 gtttccgcat ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg
    29101 gttttgtacg cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg
    29161 ttggcgtgct tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc
    29221 gcatcggtat cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta
    29281 gcaacggagg cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc
    29341 tcgccgtaac gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga
    29401 ccagcggctt ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca
    29461 atcatgctgt cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt
    29521 gcaagcgtca gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg
    29581 ctcttcacca cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg
    29641 ccgccgatat cgatgttctc aaccgcatct tccagcgagc aaccttcacg ggccacggtc
    29701 tgggcgaacg gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc
    29761 ataatggcat cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg
    29821 gtcttcacgc gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc
    29881 ggcagacctt tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg
    29941 cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga
    30001 gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc tggatttgac tattacagag
    30061 agcgttagct gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt
    30121 tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg
    30181 gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg
    30241 tggataactc tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca
    30301 gtcatttttc tgcaattttt ctattgcggc ctgcggag
//
LOCUS       DAHBHC010000062        29894 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHBHC010000062 DAHBHC010000000
VERSION     DAHBHC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.062"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1128)
                     /gene="fucO"
                     /locus_tag="M2A35_003298"
     CDS             complement(<1..1128)
                     /gene="fucO"
                     /locus_tag="M2A35_003298"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCB7199557.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVE"
     gene            complement(1156..1803)
                     /gene="fucA"
                     /locus_tag="M2A35_003299"
     CDS             complement(1156..1803)
                     /gene="fucA"
                     /locus_tag="M2A35_003299"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCB7199558.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            2350..3666
                     /gene="fucP"
                     /locus_tag="M2A35_003300"
     CDS             2350..3666
                     /gene="fucP"
                     /locus_tag="M2A35_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCB7199559.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            3699..5474
                     /gene="fucI"
                     /locus_tag="M2A35_003301"
     CDS             3699..5474
                     /gene="fucI"
                     /locus_tag="M2A35_003301"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCB7199560.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            5583..7001
                     /gene="fucK"
                     /locus_tag="M2A35_003302"
     CDS             5583..7001
                     /gene="fucK"
                     /locus_tag="M2A35_003302"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCB7199561.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            7003..7425
                     /gene="fucU"
                     /locus_tag="M2A35_003303"
     CDS             7003..7425
                     /gene="fucU"
                     /locus_tag="M2A35_003303"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCB7199562.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            7483..8214
                     /gene="fucR"
                     /locus_tag="M2A35_003304"
     CDS             7483..8214
                     /gene="fucR"
                     /locus_tag="M2A35_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCB7199563.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8258..9358)
                     /gene="rlmM"
                     /locus_tag="M2A35_003305"
     CDS             complement(8258..9358)
                     /gene="rlmM"
                     /locus_tag="M2A35_003305"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCB7199564.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(9351..9746)
                     /locus_tag="M2A35_003306"
     CDS             complement(9351..9746)
                     /locus_tag="M2A35_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCB7199565.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(9765..10682)
                     /gene="gcvA"
                     /locus_tag="M2A35_003307"
     CDS             complement(9765..10682)
                     /gene="gcvA"
                     /locus_tag="M2A35_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCB7199566.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(11033..11260)
                     /locus_tag="M2A35_003308"
     CDS             complement(11033..11260)
                     /locus_tag="M2A35_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCB7199567.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            11452..12657
                     /gene="csdA"
                     /locus_tag="M2A35_003309"
     CDS             11452..12657
                     /gene="csdA"
                     /locus_tag="M2A35_003309"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCB7199568.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            12657..13100
                     /gene="csdE"
                     /locus_tag="M2A35_003310"
     CDS             12657..13100
                     /gene="csdE"
                     /locus_tag="M2A35_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCB7199569.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(13151..13957)
                     /gene="tcdA"
                     /locus_tag="M2A35_003311"
     CDS             complement(13151..13957)
                     /gene="tcdA"
                     /locus_tag="M2A35_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCB7199570.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(14034..15131)
                     /gene="mltA"
                     /locus_tag="M2A35_003312"
     CDS             complement(14034..15131)
                     /gene="mltA"
                     /locus_tag="M2A35_003312"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCB7199571.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            15340..15416
                     /locus_tag="M2A35_003313"
     tRNA            15340..15416
                     /locus_tag="M2A35_003313"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15374..15376,aa:Met,seq:cat)
     gene            16050..16559
                     /gene="tssB"
                     /locus_tag="M2A35_003314"
     CDS             16050..16559
                     /gene="tssB"
                     /locus_tag="M2A35_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCB7199572.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKL
                     RRRRTFNPK"
     gene            16608..18146
                     /gene="tssC"
                     /locus_tag="M2A35_003315"
     CDS             16608..18146
                     /gene="tssC"
                     /locus_tag="M2A35_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HCB7199573.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFSNLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWVRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            18164..19501
                     /gene="tssK"
                     /locus_tag="M2A35_003316"
     CDS             18164..19501
                     /gene="tssK"
                     /locus_tag="M2A35_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HCB7199574.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALMSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLHALTNDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            19498..20163
                     /gene="tssL"
                     /locus_tag="M2A35_003317"
     CDS             19498..20163
                     /gene="tssL"
                     /locus_tag="M2A35_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HCB7199575.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     GVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQAHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            20176..21828
                     /locus_tag="M2A35_003318"
     CDS             20176..21828
                     /locus_tag="M2A35_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCB7199576.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLIWAGAAAIVLTVA
                     ALLIYRRRQAWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQAERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEIHVATSAETASPAQWLTQTSTQE
                     RSQQLCYLLKAESLMQWLNLNMLAALNGPETKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATENRQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAIMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            21886..22377
                     /locus_tag="M2A35_003319"
     CDS             21886..22377
                     /locus_tag="M2A35_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCB7199577.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFIFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            22568..25213
                     /gene="tssH"
                     /locus_tag="M2A35_003320"
     CDS             22568..25213
                     /gene="tssH"
                     /locus_tag="M2A35_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCB7199578.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLTALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQATEPLTSAQPESATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTASLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLQARLTALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKRL
                     AQQIMALRQEGADSTDLQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPHSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            25225..27732
                     /gene="vgrG"
                     /locus_tag="M2A35_003321"
     CDS             25225..27732
                     /gene="vgrG"
                     /locus_tag="M2A35_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCB7199579.1"
                     /translation="MGIQNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
     gene            27754..28348
                     /locus_tag="M2A35_003322"
                     /pseudo
     CDS             27754..28348
                     /locus_tag="M2A35_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            28418..28900
                     /locus_tag="M2A35_003323"
     CDS             28418..28900
                     /locus_tag="M2A35_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199580.1"
                     /translation="MKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITEAFLDTPTKS
                     YDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPE
                     AVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK
                     "
     gene            28978..>29894
                     /locus_tag="M2A35_003324"
     CDS             28978..>29894
                     /locus_tag="M2A35_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199581.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNVDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTLERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQAL"
BASE COUNT         7384 a         7871 c         7729 g         6910 t
ORIGIN      
        1 ctctacaata tcctcaagcg ttgcttcacg cgggttgcca ccggtacaaa catcatccag
       61 tgccgcctgc gccagtgccg gaatgtcttc cttgcgtacc ccaacatcac gcaaatgtgg
      121 cggaataccg acatcacggt tgagagcaaa caccgcttca acagcggcat tacgcgcctc
      181 ttccaggctc ataccttcca ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc
      241 accggtaaag tcagcgttat agcgcatgac atgcggtagc aggatggcgt ttgcaacacc
      301 gtgtggagtg ttataaaacg cgcccagtgg atgcgccata ccatgcacca accctaaccc
      361 aacattcgag aagcccatac ccgcaacata ctgcccgagc gccatttctt ctccggcatc
      421 cttatcacca gcaaccgatc ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg
      481 cagtgcatcg gttagcgccc acgcgccacg ggtaatatac ccctcaatag catgagtgag
      541 cgcatcgaca cccgtcgcag ctttcagcgc tggaggcata ccatccatca tgtcagcgtc
      601 aataaacgcc acctgcggga tatcatgcgg atcaacgcaa acaaacttgc gccgtttttc
      661 ttcgtcagtg atcacgtagt taatggtcac ttctgccgca gtgcctgctg tggtggggat
      721 tgccagaatc ggtacactgg gtttattggt cggggaaagt ccttccaggc tacgcacatc
      781 ggcaaactcc gggttgttgc tgataatgcc aatcgcttta caagtatcct gtggagaacc
      841 accaccaata gcgatcaggt aatccgcgcc gctattctgg aatacaccga gcccttcttt
      901 gacgacagta attgttgggt tgggcactac gccgtcgtaa atcgcccatg ccagccctgc
      961 agcatccatc ttatcggtca ctttcgccac cacgccgcat tgcaccagcg ttttatcggt
     1021 gacgatcagc gccttctgat aaccacggcg tttcacctca tcggttaaag ccccaacagc
     1081 accccgacca aaccatgccg tttcgttcag aatcattctg ttagccatca tccttctcct
     1141 tgttgcttta cgaaattact cttcaattcg taacccatag gttttgaatt tctccagcac
     1201 tacggcaatc tcttcatcgc tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag
     1261 gtaaagttgc gccagcactt caacttcatg cgccagccat aacgcttttt ccagattcac
     1321 ctcacaagcg ataagcccat gatgttgtaa caaagttgcc ttacgatttt tgagagcctg
     1381 cgcaacatgt tcagaaagtt cacgtgttcc aaaggtcgca taaggcgcgc aaggaataga
     1441 attaccgcca gccgccgcaa tcatgtagtg aatagcgggg atcgatcggt taagaatgga
     1501 aactgccgtg caatgaacgg catgattgtg aacaaccgcg ttggcatccg gtctgctttg
     1561 ataggccgcc atatggaaac gccattcgct tgaggggagc tttccttcct catgtttacc
     1621 gttgccatca ataaagacaa tatgcgactc cgtcagtttt tcatatggaa tgcctgtagg
     1681 cgtaatcagc atcccatcct gataacgtac actgacgttc cccgctgtcc cctggttcag
     1741 tcccaggcgg gtcatttcca ggcaagtgtc aataatctga cgagcaagtt tatttcgttc
     1801 cattagctac ctctctctga ttcaaaacag ggcaataatg ttgttccttt cacactattg
     1861 aattagccgt ttaattaccc accatcttct tcctgattaa ccagaaagaa attcacaagc
     1921 ttatattttg tgacctggtt caactaatca cagtaaataa ctgcaagttc tctttttata
     1981 accccattaa aaatgaccgc tcttaaaaat atttatcaaa acggtcattt ttctattcac
     2041 tcaagccagg aatgcccgtt ttcggcataa acaattaata cggtcatctg gtttgtgttt
     2101 tttatgattt attttctgaa acgggcatga aatttcgatt attaaagtga tggtagtcac
     2161 ataaagtcac cttccagcta ataagtgtga ccgccgtcat attacagagc gttttttatt
     2221 tgaaaatgaa tccatgagtt catttcagac aggcaaatat tcactgatat gaagcccgaa
     2281 ctcgctggtt ttgcactttt gaaaacataa ccgattacgt gcttaagctt ctgaacctaa
     2341 gaggatgcta tgggaaacac atcaatacaa acgcagagtt accgtgcggt agataaagat
     2401 gcagggcaaa gcagaagtta cattattcca ttcgcgctgc tgtgctcact gttttttctt
     2461 tgggcggtag ccaataacct taacgacatt ttattacctc aattccagca ggcttttacg
     2521 ctgacaaatt tccaggctgg cctgatccaa tcggcctttt actttggtta tttcattatc
     2581 ccaatccctg ctgggatatt gatgaaaaaa ctcagttata aagcagggat tattaccgga
     2641 ttatttttat atgccttggg cgctgcatta ttctggcccg ctgcagaaat aatgaactac
     2701 accttatttt tagttggcct atttattatt gcagccggat taggttgtct ggaaactgcc
     2761 gcaaaccctt ttgttacggt attagggccg gaaagcagtg gtcacttccg cttaaatctt
     2821 gcgcaaacat ttaactcgtt tggcgcaatt atcgcggttg tctttgggca aagtcttatt
     2881 ttgtctaacg tgccacatca atcgcaagac gttctcgata aaatgtctcc agagcaattg
     2941 agtgcgtata aacacagcct ggtattatcg gtacagacac cttatatgat catcgtggct
     3001 atcgtgttac tggtcgccct gctgatcatg ctgacgaaat tcccggcatt gcagagtgat
     3061 aatcacagtg acgccaaaca aggatcgttc tccgcatcgc tttctcgtct ggcgcgtatt
     3121 cgtcactggc gctgggcggt attagcgcaa ttctgctacg ttggcgcaca aacggcctgc
     3181 tggagctatt tgattcgcta cgctgtagaa gaaattccag gtatgactgc aggctttgcc
     3241 gctaactatt taaccggaac catggtgtgc ttctttattg gtcgtttcac cggtacctgg
     3301 ctcatcagtc gcttcgcacc acacaaagtc ctggccgcct acgcattaat cgctatggca
     3361 ctgtgcctga tctcagcctt cgctggcggt catgtgggct taatagccct gactttatgc
     3421 agcgccttta tgtcgattca gtacccaaca atcttctcgc tgggcattaa gaatctcggc
     3481 caggacacca aatacggttc gtccttcatc gttatgacca tcattggcgg cggtattgtc
     3541 actccggtca tgggttttgt cagtgacgcg gcgggcaaca tccccactgc tgaactgatc
     3601 cccgcactct gcttcgcggt catctttatc tttgcccgtt tccgctctca aacggcaact
     3661 aactgaacat attttccgaa taaagtgagg aatctgtaat gaaaaaaatc agcttaccga
     3721 aaattggtat ccgcccggtt attgacggtc gtcgcatggg tgttcgtgag tcgcttgaag
     3781 aacaaacaat gaatatggcg aaagctacgg ccgcactgct gaccgagaaa ctgcgccatg
     3841 cctgcggagc tgccgtcgag tgtgtcattt ccgatacctg tatcgcgggt atggctgaag
     3901 ccgctgcttg cgaagaaaaa ttcagcagtc agaatgtagg cctcaccatt acggtaacgc
     3961 cttgctggtg ctatggcagt gaaaccatcg acatggatcc aacccgcccg aaagccattt
     4021 ggggctttaa cggcactgaa cgtcccggcg ctgtttacct ggcagcggct ctggcagctc
     4081 acagccagaa aggcatcccg gcattctcca tttacggtca tgacgttcag gatgccgatg
     4141 acacatcgat tcctgccgat gttgaagaaa aactgctgcg ctttgcccgc gccggtttgg
     4201 ccgtcgccag catgaaaggt aaaagctatc tgtcgctggg cggcgtttcg atgggtatcg
     4261 ccggttctat tgttgatcac aacttctttg aatcctggct gggaatgaaa gtccaggcgg
     4321 tggatatgac cgaactgcgt cgccgtatcg atcagaagat ttacgacgaa gccgaactgg
     4381 aaatggcact gacctgggct gataaaaact tccgctatgg cgaagatgaa aataacaaac
     4441 agtatcaacg caatgccgag caaagccgcg cagttctgcg cgaaagttta ctgatggcga
     4501 tgtgtatccg cgacatgatg caaggcaaca gcaaactggc cgatattggt cgcgtggaag
     4561 aatcacttgg ctacaacgcc atcgctgcgg gcttccaggg gcaacgtcac tggaccgatc
     4621 aatatcccaa tggtgacacc gccgaagcga tccttaacag ttcatttgac tggaatggcg
     4681 tgcgcgaacc ctttgtcgtg gcgaccgaaa acgacagtct taacggcgtg gcaatgctaa
     4741 tgggtcacca gctcaccggc accgctcagg tatttgccga tgtgcgtacc tactggtcac
     4801 cagaagcaat tgagcgtgta acggggcata aactcgatgg actggcagaa cacggcatca
     4861 tccatttgat caactccggt tctgctgcgc tggacggttc ctgtaaacaa cgcgacagcg
     4921 aaggtaaccc gacgatgaag ccgcactggg aaatctctca gcaagaggct gacgcttgcc
     4981 tcgccgctac cgaatggtgc ccggcgatcc acgaatactt ccgtggcggc ggttactctt
     5041 cccgcttcct taccgaaggc ggcgtcccgt tcaccatgac ccgtgtcaac atcatcaaag
     5101 gcctgggacc ggtactgcaa atcgcggaag gctggagcgt ggaattgccg aaggatgtgc
     5161 atgacatcct caacaaacgc accaactcaa cctggccaac cacctggttt gcaccgcgcc
     5221 tcaccggtaa agggccgttt acggatgtgt actcggtaat ggcgaactgg ggcgctaacc
     5281 atggggttct gaccatcggc cacgttggcg cagactttat cactctcgcc tccatgctgc
     5341 gtatcccggt atgtatgcac aacgttgaag agaccaaagt gtatcgtcct tctgcctggg
     5401 ctgcgcacgg catggatatt gaaggccagg attaccgcgc ttgccagaac tacggcccgt
     5461 tgtacaagcg ttaatacatt actccctgat gtgatgcccg gtcgctccgg ctaccgggcc
     5521 tgaacaagca agagtggtta gccggataag caatgttatc cggctatatt gcaggagcga
     5581 ttatgaaaca agaagttatc ctggtactcg actgtggcgc gaccaatgtc agggccatcg
     5641 cggttaatcg gcagggaaaa attgttgccc gcgcctcaac gcctaatgcc agcgatatcg
     5701 cgatggaaaa caacacctgg caccagtggt ctttagacgc cattttgcaa cgctttgctg
     5761 attgctgtcg gcaaatcaat agtgaactga ctgaatgcca catccgcggt atcgccgtca
     5821 ccacctttgg tgtggatggc gctctggtag ataagcaagg caatctgctc tatccgatca
     5881 ttagctggaa atgtccgcga acagcagcgg ttatggacaa tattgaacag ttgatctccg
     5941 cacagcggtt gcaggctatt tctggcgtcg gagcctttag tttcaatacg ttatataagt
     6001 tggtgtggtt gaaagaaaat catccacaac tgctggaacg cgcgcacgcc tggctcttta
     6061 tttcgtcgct gattaaccac cgtttaaccg gcgaattcac tactgatatc acgatggccg
     6121 gaaccagcca gatgctggat atccagcaac gcgatttcag tccgcaaatt ttacaagcca
     6181 ccggtattcc acgccgactc ttccctcgtc tggtggaagc gggtgaacag attggtacgc
     6241 tacagaacag cgccgcagca atgctcggct tacccgttgg cataccggtg atttccgcag
     6301 gtcacgatac ccagttcgcc ctttttggcg ctggtgccga ccaaaatgaa cccgtgctct
     6361 cttccggtac atgggaaatt ttaatggtcc gtagcgccca ggttgatact tcgctgttaa
     6421 gtcagtacgc cggttccacc tgcgaactgg atagccaggc agggttgtat aacccaggta
     6481 tgcaatggct ggcatccggc gtgctggaat gggtgagaaa actgttctgg acggctgaaa
     6541 caccctggca aatgttgatt gaagaagctc gtctgatcgc gcctggcgcg gatggcgtaa
     6601 aaatgcagtg tgatttattg tcgtgtcaga acgctggctg gcaaggagtg acgcttaata
     6661 ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca
     6721 atctacagat gctggaaaaa atcgggcact ttaaggcctc tgaattattg ttagtcggtg
     6781 gaggaagtcg caacacattg tggaatcaga ttaaagccaa tatgcttgat attccggtaa
     6841 aagttctcga cgatgccgaa acgaccgtcg caggagctgc gctgttcggt tggtatggcg
     6901 taggggaatt caacagcccg gaagaagccc gtgcacagat tcattatcag taccgttatt
     6961 tctacccgca aactgaacct gaatttatag aggaagtgtg aaatgctgaa aacaatttcg
     7021 ccgttaattt ctcccgaact attgaaagtg ctggcagaga tgggacatgg agatgaaatt
     7081 attttttccg atgctcactt tcccgcccat tcgatgggac cgcaggtgat ccgcgctgat
     7141 ggcctgttgg tgagcgactt gctccaggcg attatcccgt tatttgagct ggacagttat
     7201 gccccgccgc tggtgatgat ggcagcggta gaaggtgaca ctctcgatcc tgaagtagaa
     7261 cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat
     7321 cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga
     7381 gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac cgtaatctca taccggtacg
     7441 cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag
     7501 caagcgatag tcgacctgct gctgaaccat accagcctga ccacggaagc tctctctgaa
     7561 cagctaaagg tcagtaaaga aaccattcgt cgcgatctca atgaattaca gacgcagggt
     7621 aaaattctgc gcaatcatgg acgcgctaaa tatatccacc gtcaaaatca agacagtggc
     7681 gatccctttc acatccggct gaaaagccat tatgcgcata aagcagatat cgcgcgcgag
     7741 gcgctcgcgt ggattgaaga agggatggtg atagccttag acgccagttc aacttgctgg
     7801 tatctggcac gccagttgcc tgacatcaac attcaggtct tcaccaatag ccatccgatt
     7861 tgccatgaac tcggtaaacg cgaacgcatt caactgatca gttccggcgg cacacttgag
     7921 cgcaaatatg gctgttacgt caatccctcg ctgatttccc aacttaaatc gctggaaatc
     7981 gatctgttta ttttttcttg tgaagggatc gatagcagcg gcgcactgtg ggactccaat
     8041 gccatcaacg ctgattacaa atcgatgcta ttaaaacgtg ccgcgcaatc gttgttattg
     8101 attgataaaa gtaaatttaa tcgttcaggg gaagcccgca tcgggcatct ggatgaggta
     8161 acgcacatta tttctgatga gcgccaggtt gcaacttctt tggtaacagc ctgacggaaa
     8221 gggtagcagg ccggagacga cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac
     8281 ccaccgcagc ccagatgcgg cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact
     8341 gccgcgcctg aatctgagca tttatgccat gttcatcaag ctgtgcctga atatacgcca
     8401 gattgtgtga tacttcttcg tagcgttttt tcatcggcag tttgagattg aaaatggttt
     8461 cacggcacca gccattaacc agccactgcg ccatcaatgc cgcaacttta gccggttttt
     8521 caaccatatc gcataccatc caggagatat tgctgcgtgt cggacggaat ttgaaaccgt
     8581 cttcccgcag ccacgtcacc tgcccggtat ccatcagact ttgcgccatc gggccgttgt
     8641 cgacggaata aacccacatg ttgcgcttca ccagttggta ggtccagccg cctgggcaag
     8701 cgcccaaatc caccgcccac atcccgttcg ccaggcgttc atcccactca tccgcaggaa
     8761 taaacacatg aaatgcctct tccagtttga gtgtggaacg actcggcgca tctgccggaa
     8821 atttcaggcg cggaatgccc atatagaacg gcgaattatt gttgctgtat gagtaaccgg
     8881 tatagcagca acctggtgca atgaagaata catgcacaac cggacgcttc ggcgtttcat
     8941 agttcgccag caccccggca tcgcgcaagg cagcgcgtag cggcacggtg aatttacggc
     9001 agaatttcag caactctttg ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc
     9061 ccttctctac tacgccctgt aacatgccga caatgggggt aatacgatct tctggcggca
     9121 aatgctgcag gagttccccc accacaaacc actggcgggc aaaaattaat gaactgaacg
     9181 gcagctcacg gattaactta tcgccatcat caggttgata acattcataa atgacataac
     9241 ccgcattctc tttcacgcgg gcaaaaccga aaatttcccg ctggccggct ttatcggtaa
     9301 tttctgcggc gcactctttt tcaaagcccg gacggcacag caatacaacc ttattcatga
     9361 cttacgccct tgcgctttaa acggatagca ccaactaaca ttaacgccca gcccgcaagg
     9421 aagctcacgc cgccaaccgg agtgacaaac gcccacaaac gcagatggga cagtgccagg
     9481 caataaaggc tgccgctgaa cagcaccgtg cctaacgcga ggaaaacgct actccagtaa
     9541 aaccagatac tgatgcgacg ctgcattgcc accgccagac ctaagatcgc cagcgtatga
     9601 aacgcctggt attcgaggcc ggtctggatc cagcccatct caacggcccc catggtttta
     9661 cttaacacat gcgcgccaaa agcgcccaga gccacaaaaa tgaagccgct aatggcggcg
     9721 aaaatcagca taaaacggct ggtcatggtc gtaccctacg taaattattg ttcataacga
     9781 aagcggaatt tttcttgttc agcagcggct ttcgccagga tccattggcg aaaggcggct
     9841 attttaccca gttctgcctg gctgtcatga caaaccagat aaaaagcgtt tttactgacc
     9901 agaacatcat taaacgggca aacaagacgt ccggcttcaa tttcagattg cgccattacg
     9961 ttatttgcca gcgccactcc ctgcccgtga atagccgctt gcagcaccat ggcgctatga
    10021 ctaaaaattg gcccttgctg aacgttgata tgatttaacc ccaactgtcg ggtatatgtc
    10081 tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt tagccagatc ttccggggtc
    10141 ttcaagggtt tttcgcctgt cagcagtagc ggcgaacaca ccggcaataa atattcggcg
    10201 tacagttttt ccacccgtag ccccggccag ttgccccgac cataaaatat cgccacatca
    10261 acatcatccg ccagcttatc ttcctgacga tcaaccgcct ggattcgaac gtcaattccc
    10321 ggataagctg aattaaagct ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg
    10381 agtaaactga ccgtcaacgc ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg
    10441 gttaattgcg aaaatatctc tttgatatcg aggaaatagc tttgcccttc ctcggtcagc
    10501 aggagtgaac gattacggcg gcggaacagt tttagcccca aaaaatcctc aagagacttg
    10561 atttgatgac ttactgcggc ctgggtcaca aaaagctctt ctgctgcgcg agtgaaactt
    10621 aaatggcgtg ctgcggcatc aaaaactcgt aaggcattta gcggtggtaa tcgtttagac
    10681 atggctttta aactttgatg ttaaatgaat ttaacaatta gatcacactg tgtaacctat
    10741 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
    10801 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa
    10861 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
    10921 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
    10981 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgtg aattactgat
    11041 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
    11101 ctttatacga aatcataccg gtatcgttat cagtctgtgg tttgccgtca gagacgatgg
    11161 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
    11221 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaagcgat
    11281 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
    11341 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
    11401 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
    11461 tttaatcccg cgcagtttcg cgcccagttt cccgctctac aggatgcggg cgtctatctc
    11521 gacagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccacccg acagttttac
    11581 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
    11641 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
    11701 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
    11761 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
    11821 gtcccctggc tgatggtcgc ccaacaaact ggagccaaag tggtgaaatt gccgcttaat
    11881 gcgcagcgac tgccggatgt cgatttgttg ccagaactga ttactccccg tagtcggatt
    11941 ctggcgttgg gtcagatgtc gaacgttact ggcggttgcc cggatctggc gcgagcgatt
    12001 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg ggcggtgcat
    12061 ttccccgcag atgttcagca actagatatc gatttctatg ctttctctgg tcacaaactg
    12121 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
    12181 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggctt cacgactcaa
    12241 tctgcgccgt ggaaactgga agctggaacg ccaaatgtcg ctggtgtcat aggattaagc
    12301 gcggcgctgg aatggctggc agattacgat atcaaccagg ccgaaagctg gagccgtagc
    12361 ttagcaacgc tggcggaaga tgcgctggcg aaacgtcccg gctttcgttc attccgctgc
    12421 caggattcca gcctgctggc ctttgatttt gctggcgttc atcatagcga tatggtgacg
    12481 ctgctggcgg agtacggtat tgccctgcgg gccgggcagc attgcgctca gccgctactg
    12541 gcagaattag gcgtaaccgg cacactgcgc gcctcttttg cgccatataa tacaaagagt
    12601 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
    12661 caaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
    12721 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
    12781 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
    12841 aaaaccgcgt ctggctggga tataccgtga ctgaaaacgg taaaatgcac ttctttggcg
    12901 acagcgaagg gcgcattgtg cgcggcctgt tggcggtgtt gttgactgcc gttgagggga
    12961 aaaccgtcgc cgaattgcag gcacagtcgc cgctggcatt gtttgatgag ctgggattgc
    13021 gtgcacagct tagcgcctca cgcagccagg ggttgaatgc gttaagcgag gcgattatcg
    13081 ctgcggcgaa gcaggtttaa gcacagaacc aggtcggata agacgttcgc gtcgcatccg
    13141 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
    13201 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
    13261 ccatccgctt cggcccttct gccgtggctt tcatcgcaca cacagtaccg tctgactgcg
    13321 ggtacaccag cgcttcagta gaaaacacgc aatcgacgcc gagcttacct ttactgtttt
    13381 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
    13441 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
    13501 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
    13561 gtacactatc aatggcatca atcacgtacg aataacccac gctcatatac tgcgctacgt
    13621 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
    13681 tacgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
    13741 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
    13801 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
    13861 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
    13921 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggt
    13981 gaggttatcc ctcaccctgg cgtatccgta aaaacggtat acagaataac acatcagccg
    14041 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggttg
    14101 taccaacctg cgcgatgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
    14161 cctttgattg cgccaccgac atccagcgcc accatcagac gcaattcgta ctgaccgtta
    14221 aatttgccgt tattatccag cagcggcact tctgccagca aggtagtacc tggcggaata
    14281 attgaacgat cggaggcaac agaagcacgg ccaaccagcg gcaccgcact tgcccctttc
    14341 accggtgcaa aagattgcgg tttaaagaaa acgaaagacg ggttctgttc cagcagctcg
    14401 cgaacctcgg cttcactgtg tgtttcgccc caatgacgaa tcgcctgcat cgacatatct
    14461 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
    14521 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatatacccg
    14581 ctaccctgca catccataat gaagttatcc atcagggagt tgctgtaagc gagaatatat
    14641 ttatcactca gcgcccctgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
    14701 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
    14761 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
    14821 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
    14881 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
    14941 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
    15001 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
    15061 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
    15121 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
    15181 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgtacaa aaaataggct
    15241 ttagtggttt gtttttgttc aaaatcatgc taaatccgtg atcggggtaa aaaaaaggtt
    15301 gcatgaaaac gcgagcggag tatagtgcgc atccacggac gcggggtgga gcagcctggt
    15361 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaattt
    15421 attagaatat cagaaaccgc aacgctacct ttagcttgcg gttttttcgt ttttacgctt
    15481 ccttatccgt ttcagtgaag cctgaaacaa tgagtaaacc gttgatcccc cccaatcaaa
    15541 gccatccatc ttatttatat attaaatata taccccacca atgaacttca cttacatatt
    15601 ctataaatca atttgttttg attatgttaa catttcaata tcacaactgg aggctacact
    15661 tatataatta ttgtacaaaa agcacacatt ttttaattag tagaaaaatt aataattttt
    15721 tgtggaagcc cgggcaataa ttttaatgaa tgctttaata gactcccaca ctacccaatt
    15781 tcttaaaaac gcaaatttgt gaagtttatc ttgttattat tttaagtttc atgccaaatt
    15841 ttctcaccag ataaggctgc cctctcccga aaaataatca agaggctaaa taatatctaa
    15901 aatgatacaa ccgtatctat tccccagaaa aatacattat tcatttttat attttcctaa
    15961 tcattgcttt ttatttaaat cacccgataa tcccgtgaat ataattatgt caataaccat
    16021 cagaaaaagt ggatgatgag gaaaaggata tggccgacag tttccagaat gaagttcccg
    16081 ccgctcgtgt aaatatcaag cttgacctgc atacaggcaa tgctaaaaag aaagttgaac
    16141 tccccctcaa gcttcttgct gttggcgatt acagtaacgg aaaagagcaa cgtccgctgt
    16201 ccgaacggga caagattgat atcaataaaa acaacttcaa cagtgtcatg gctgagtttt
    16261 cgcctgcggt taatttaaca gtagaagaca cactaagcgg aagcggtaat gagcaaaata
    16321 tcgcgcttga atttaaaagc ctgaaagatt ttgaacccga acaggtagca aaaaatatcc
    16381 cacagcttcg tgtattactc gctatgcgca atttattacg tgacctgaaa tccaatctgc
    16441 tggataacgc aacgtttcgc cgtgaactgg aaaatattct gaaagaccca acgctcagtt
    16501 cagaactgcg tgatgaactg gcgaaattgc gccgcaggag aacgtttaac ccgaagtgat
    16561 attcaggtta aaccggagaa gaaaggatca ctgaaaagga aatgctgatg tctgtacaac
    16621 aagaacatgc cacctctgaa actgcaacac tgaccaccac tgagtccggc ggcgtttatc
    16681 agtccctgtt tgataaaatt aatttaaccc cggtgtcttc cattcaggaa atagatttat
    16741 ggcaaaacag cgaaacactg gcggatgcct cacccgatga gcgcgtgaca gcggcgattc
    16801 acgttctgct ttcctgtctg gcgaaatcag gcgagaacgt ggtcaagctc gacaagagcc
    16861 tgctggattt tcatatcgac gatttggatc agaaaatcag taagcagctt gatgcggtca
    16921 tgcaccatcc tgaattccag aaagtcgagt cgctgtggcg cggcacatgg ttcgtcgtac
    16981 agcgcactga ttttcgcaaa aatgtcagaa ttgaattgct ggatatcagc aaagagcatc
    17041 tgcgtcagga tttcgacgac tccccggaaa tcattcagag tggtttatat cgtcatacat
    17101 atattcagga gtacgatacg ccgggtggcg aacctgttgc ctcattaatt tccagctatg
    17161 aatttgataa cagcccgcag gatattgccc tgctgcgcaa tatttccaga gtgtctgccg
    17221 cttcccatat gccttttatc ggttctgtcg ggccgaaatt cttccttaaa aattcgatgg
    17281 aagaagtcgc cgcgattaaa gatatcggca actactttga ccgcgcagaa tatattaaat
    17341 ggaaatcgtt tcgtgatacc gatgacagcc gctatgtggg attagtgatg ccgcgagtgc
    17401 tgggccgtct gccctatggg ccggacacgg tgccggtacg cagctttaac tatgtggaag
    17461 aagtcaaagg cccggatcac gaaaagtatc tctggacaaa cgcctcgttc gcctttgccg
    17521 ccaatatggt gaagagcttt gtgaataatg gctggtgcgt gcagatccgc ggtccacaag
    17581 caggtggcgc agtggccgat ctgcccatcc atctttacga tctcggcacc ggcaatcagg
    17641 tcaaaattcc gtccgaagtg atgatcccgg aaacccgcga atttgaattt tccaaccttg
    17701 gctttattcc gctctcttat tataagaatc gcgattacgc ctgcttcttc tcggcaaact
    17761 ctgcccagaa accggcgttg tatgataccg ctgacgccac cgccaacagc cgtattaacg
    17821 cccgtctgcc ctatatcttc ctgctgtccc gcattgcgca ttacctgaaa atcatccagc
    17881 gcgagaatat cggtaccacc aaagaccgcc gcgtactgga actggaactg aacacctggg
    17941 tccgcacact ggtgacggag atgaccgatc cgggtgatga actgcaggcg tctcacccgc
    18001 tgcgcgacgg taaggttatc gtggaagata ttgaggacaa tccgggcttc ttccgcgtca
    18061 gactctttgc cgtgccgcat ttccagattg aagggatgga tatcaacctg tcactggttt
    18121 cccagatgcc gaaagcgaaa gcctgaccgg gaagtggtgt gatatgaaaa ttcatcgacc
    18181 actgtgggct gaggggacat tcctgtcctc tcagcagttt cagcagcagg cccgctggga
    18241 agcgttcagc aatgacagca ttgcgcaact ttgtattcgt catccgtggg ggatcgcgaa
    18301 tgtgctcttt gaccgcgatg ccctgatgtc cggaaagctg aaaacgcagg cagtacgcct
    18361 gcgttttgct gatggcacgc tgatcgattc tgacgtgagc gatgttctgc ctctcgcctg
    18421 cgatctgcac gcgctgacga acgacagcgc ggttgttctg ctggcgctgc cgcttgccca
    18481 tggtaacggc ggtaatctcg gtcagggtga gcagactgaa agaccgctac gctatcgtca
    18541 ggaatggcaa aaggtacagg atatctacgg cagtgacagt gaagatatgg ccgttgagcg
    18601 tcatgcgctg tccctgcgct ttgctcatga taacaatcag gattacatca cctgcccact
    18661 ggcgcgtctg gtgcgggatg ttcagggcaa ctggacacag gatgagggct atattccgcc
    18721 gttactggcg tttaatgctc atgacgggtt ggtacagcgt ctggacacgc tgctgttgca
    18781 gctacgggcc aaatgtcagc gcctgatggc gatgcgtcgg gaaagcaacc agcgtatggc
    18841 ggacttcgcc gtcgccgatg tctccctgtt ctggttgctt aacgcgctga actccgccga
    18901 gccggtgctg agtgatttcc tgcgctaccc ggcggttcat cccgaactgg tctggcggga
    18961 gctggcgagg cttgccggag cgctgctgac gttttccctt gaacataacg tcagcgccgt
    19021 tccgccctat gtccacgaat cgccgtccac tgtctttccg ccactgttct ccctgctcag
    19081 cgaactgctg gaagcaagcc tgccgtcacg ggtgatcgcg ctggatctgg gatccctgcc
    19141 gggcaaccgc tggaaagccg acctgcacga tccgcgcctg cgggaagagg ccgattttta
    19201 cctgtccgtt cgttcctcac tgcctgcgca tcaggtactg caccagctac cgctggtctg
    19261 caaaatcggt gcgcctgatg acgtcacgct gcttatcaac gtggcgctga atggtgttcc
    19321 actggtgcca ctgacctcag ttccggcagc gctgccactg cgcctggaga atcagtattt
    19381 tgcgctggat atgcacagtg acgcggctaa atcgatgctg gagtccgggt gctgcatgat
    19441 ttatgcgcca ggaaccatgg gcgacctgaa acctgaactg tttgcggtgc tgcgcacatg
    19501 aaaaaagaga cggatatcaa tatcgacgcg ctgctgcgcg acacgtttct gacggtggtg
    19561 gaactgcgtc agggaaccac cgtgcgtcac ggtatggagc tgtatcgcca ctgccagcgg
    19621 caggtggagg gggtccgcga acgcctgaaa gatgccgggt tcagccggga gaacgtggag
    19681 cacattactt acgcacagtg cgccctgctg gacgaaacag tcctgagtcg cagcggcatg
    19741 gacgacggtc aggccatctg gatgaaggat ccactccagg cgcatttctt caacaccctg
    19801 caggcaggcg agctgctgta cgagcggatg aagcaggtgt tgcaggagcc cgcgcctgca
    19861 caggcagtgc tgacctgctt tcaccgtgtc ctgctgctgg gatttcgcgg gcgttatcag
    19921 gatccggtgg ccccggagcg tgaccaactt atttcgacgc tcaacgggct ggtcgcgcca
    19981 tttggcgtgc tgccggaaac ggcggtactg aacgtgccgt tatccccccg tcagcaccct
    20041 ttgctgcatt cgccgttttt ctggctggta acgctgacgc tgctactggc cggtgtctgg
    20101 tggggactgc accactggct gaacgtgctg gtggatgaat tgctgccgca aagcctgcgg
    20161 taaagggagt cgtgagtgag gaacacgctg aaacaggccg tcgtgctgtg gggaatggtg
    20221 ttactgctgg tgctgtggtc ggtgtttatc agtccgtctg gcgtactgat atgggccggg
    20281 gcggcggcta tcgttctgac ggttgccgcg ttgctgattt accggcgcag gcaggcgtgg
    20341 acggagatga ccggcgatgc cgggttgtta tcgctgccgc cggaaacgta ccgacaaccg
    20401 gtagtgctgg tctgtggcga tatgtcggcg catctgttca ctgacagccc ggtccggcag
    20461 gtttcagaag ggctgtatct gcatgtttct gatgaagaac agcttgtggc gcaggcggag
    20521 cgattactga cccttcgccc ggcgtgggca tcgcagcttg ccgtggcata taccgtcatg
    20581 cccggcatgt accgggatgc ggcggttctg accggacaac tgcgccggtt cgcccacagt
    20641 atggcgacag tgcgtcgccg ggcgggcgta aacgtcccct ggcttctctg gagcgggctg
    20701 tccggctcgc cgttgccgga gagagcgaat tcaccgtggt ttatctgtac cggcggcgaa
    20761 attcatgtag cgacgtctgc tgaaaccgcc tcccccgcgc agtggctcac gcagaccagc
    20821 acacaggagc gcagccagca actctgttac ctgctgaaag ccgaaagcct gatgcagtgg
    20881 ctgaatctta atatgctggc ggcactgaac ggcccggaaa cgaaatgtcc gccactggcg
    20941 atggccgtgg ggctggtccc ctcgttgcct gcggtggata acaacctgtg gcagttgtgg
    21001 atcaccgcca gaaccggcct gacgacggat atcgcggaca ccggcacaga cgccacgctg
    21061 ccgtttccgg atgccctgtt acggcggttg ccgcgtcagt cgggctttac cccgctgcga
    21121 cgagcctgcg tgaccatgct gggcatcacc accgtggcgg gtatcgccgc gctgtgtctg
    21181 tcagccacgg agaatcgcca gttattacgg catatcggtg atgatctgca ccagttttat
    21241 gccgttccgg cggaggaatt tatcaccaaa gcccgtcgtc tgtcggtgct gaaagacgat
    21301 gcgatcatgc tcgatgggta ttaccgggaa ggagagccac tgcgtctcgg tctggggtta
    21361 taccccggtg aacaaatccg ccagccggta ttacgcgcca ttcgtgactg gcgtccgcct
    21421 gagcaaaaaa tggaagtgac agcctcgctt caggcgcaga ccgtgcgtct tgacagtatg
    21481 tcgctgtttg acgtcggaca ggcccgcctg aaagacggct cgacaaaagt gctggtggac
    21541 gcactggtga acatccgggc aaaaccgggc tggctgatcc tcgtggccgg atataccgac
    21601 gccaccggcg acgaaaaaag caatcagcag ttatcgctgc gacgtgccga agcggtgcgc
    21661 aactggatgc tgcagaccag cgacatcccg gccacctgtt ttgccgtaca gggactgggc
    21721 gagagccagc ctgcggcgac caacgatacg ccacagggcc gggcagtcaa ccggcgcgtc
    21781 gaaatcagtc ttgttccgcg ttctgacgcc tgtcaggacg tgaaataaaa cataccgccg
    21841 gaagaaggcg gtgcttcaat cacactaaca aggagagtaa ttctcatggc tattcctgct
    21901 tatctctggc tgaaagatga cggcggcgcg gatatcaaag gttccgtgga cgttcagggg
    21961 cgcgaaggca gcatcgaagt ggtggcgctg gatcacgatg tgtacatccc gaccgacaac
    22021 aacaccggca aactgaccgg tactcgcacc cataaaccgt tcatttttac caaagaaatc
    22081 gatgcgtcca gcccgtatct ctacaaagct gtgaccaccg gtcagaccct gaaaacggcg
    22141 gaatttaagt tttaccgcat caacgacgcc ggtcaggaag tggagtactt caacatcacg
    22201 ctggataacg tcaagctggt cagagtcgct ccgctgatgc acgacatcaa ggagccttcc
    22261 aaagagaagc ataaccacct ggaacgcatt gagttccgct acgaaaaaat cacctggact
    22321 tacaaagacg gcaacatcat tcattccgac tcgtggaatg aacgtccttc cgcctgataa
    22381 ctgcggcaga caggaaactg tctgcctttc tgttgtgtgc tttgcccagt gccgcaggca
    22441 ccggtactga gcaaagcata cccacaatct ggtgatacct gatgggcctg agtgaggtaa
    22501 cggctatggg cggtagcgta cctgaacgca gatcgcagga atacagaaca cgataaggaa
    22561 accttctatg accggaaatc accccgccgc gctgctgcgt cgccttaacc cgtactgtgc
    22621 acgggcgctg gacgctgccg cctcactgtg tcagacccgc gcccatgcgg agataaccat
    22681 tgaacactgg ctgctgaaac tgctggagca gggagaaggc gatatcacgg tgattgcccg
    22741 ccgctatgaa tgggatatcg acacgctctg gcagtctctg ctggcacatc tggacacctt
    22801 accccgctcg gtccgcgaac gtccgcagct ttctgaaccc ctgacggcgc ttatcaggca
    22861 ggcgtggctg attgcctcgc tggaaggcga cgacccgcaa atccgcagtc agcacctgct
    22921 gatggcgctg acagaaaaac cgatgctgcc cgcctgtaat gacctgtggg tattgctgag
    22981 cctgagccgc gtgcagcttg agcggctgcg ccccctgctg gatgcgcagt cggatgaatg
    23041 tccggcacgt cagccacagg ccaccgaacc gctgacatcc gcgcaaccag agtcggcaac
    23101 gacggacgca ccggcaaaaa cgctgacggg gaaacaggat gacgccctgc tggcggtact
    23161 taaccgcttt accgaagacg tgacggaaaa agcccgcagc gggcgaatcg acccggtatt
    23221 cgggcgcgac accgaaattc gccagatggt cgatatcctc tcccgtcgcc gcaaaaacaa
    23281 cccgattctg gtgggagaac cgggcgtggg caaaaccgcg ctggtggaag ggctggcgct
    23341 gcgtatcacc gaaggcaacg tgccggacag cctgaaaacg gtgcatatcc gcacactgga
    23401 cctcggtctg ttacaggctg gcgcgggcgt taaaggtgaa tttgagcagc gactgaaaaa
    23461 cgtgattgat gcagtacaga aatcaccgga gccggtactg ctgtttattg atgaagccca
    23521 taccattatc ggcgcgggta atcaggcggg cggcgcggat gcggcgaacc tgctgaaacc
    23581 ggcgctggca aggggcgagc tgcgcaccat cgccgccaca acgtggagcg aatacaaaca
    23641 gtattttgag cgcgacgccg cgctggaacg ccgcttccag atggtgaaag tcgacgagcc
    23701 ggatgatgac accgccagcc tgatgctgcg gggactcaag gcccgctatg cacagcacca
    23761 cggcgtacat atgctggaca gcgccattca gaccgccgtg cgcctgtcgc gccgctatct
    23821 gaccggacgc cagttgccgg acaaggcggt tgatttgctg gataccgccg gggcaagagt
    23881 ccgcatgagc ctcgacaccc tgccggaacc gttgacgcag cttcaggcgc gtctgaccgc
    23941 gctggatatt gagcgggaag cgattgagca ggacagcgtg ttttatcccg aaaccagccc
    24001 ggagcggctg gcggaactga ccgatttgcg tgatgagcta caggcagaag ccgggcatct
    24061 ggaagcgcag tatcagcagg aaaagcggct tgcgcagcag attatggcgt tgcgtcagga
    24121 aggggcagac agcactgatt tgcaacagca actgcgaacg catcagggct ttgcaccgct
    24181 gctggcgctg gatgtggacg cccgcgccgt cgccactgtg gtggcggact ggaccggcat
    24241 cccgcactct tccctgctca gggacgagca gagcgacctg ctcagtatgg aacaaagcct
    24301 tgaaaaccgc gttgtcgggc aaagcccggc gctctgcgcc atcgcacagc ggctgcgggc
    24361 ggctaaaacc ggcctcacgc cggagaacgg cccgcagggg gtattcctgc tgaccggccc
    24421 cagcggcacc ggtaaaaccg aaactgcgct cacactggcc gacaccctgt ttggcggtga
    24481 aaaatccctt atcaccatca acctctccga atatcaggag ccgcataccg tctcccagct
    24541 taaggggtct cctccgggct acgttggcta cggtcagggc ggcgtactca ccgaagccgt
    24601 tcgcaaacgc ccgtacagcg tggtgctgct cgacgaagtg gaaaaggcgc atcgcgacgt
    24661 gatgaacctg ttctatcagg tatttgatcg gggctttatg cgcgacggcg aaggccggga
    24721 aattgacttc cgcaacaccg tgattctgat gacggctaat ctgggcagtg accacatcat
    24781 gcagctgctg gaggaaaaac cggacgccac ggacgcagac ctgcatgaac tgctgtaccc
    24841 cctgctgcgc gagcacttcc agcctgcact gatggcgcgt tttcagacgg tgatttaccg
    24901 ccctctggga caggaagcga tgcgcgccat tgtggaaatg aaactggcgc aggtggttcg
    24961 tcgtcttcgt cagcactatg ggctggaaac ggaaatcaac gacagcctgt atgacgcgct
    25021 gaccgccgcc tgcctgctgc cggacaccgg ggcgcgtaat atcgacagcc tgctgaacca
    25081 gcaaatcctg ccggtcttaa gccagcagtt gctggcgcaa caggccgcgc accgtaaacc
    25141 agcccagcta cggcttggct gggatgagga agacgggatt gtactggagt tcgctacgga
    25201 agagatgcaa taacggaggc tgtcatgggt attcagaatt tcgatcattc acaccacaag
    25261 ctcaaaattc gtggcctgca aagtccggtg gatgtgctga ccttcgaagg ccgcgaacaa
    25321 ctgagtaccc cgttccgcta cgacattcag tttaccagca gcgataaagc catcacgccg
    25381 gagtcggtgc tgatgcagga cggcgcattc agcctgacgg caccgccggt acaggggatg
    25441 ccggtacaga ctgccctgcg caccctgcac ggcgtcatca ccggctttaa acacctgtca
    25501 tcgtcacagg atgaagcccg ttacgaagtg cgccttgaac cccgaatggc gctgctggcc
    25561 cgcagccgcc agaacgccat ttaccagaac cagacggtgc cgcaaatagt cgaaaaaatc
    25621 ctgcgcgagc ggcaccagat gcgcggtcag gacttcgtgt ttaacctcaa aagcgaatac
    25681 ccgtcccgtg aacaggtgat gcagtacggt gaggacgacc tgacgtttat ttcacgcctg
    25741 ctgtcggaag tgggtatctg gttccgcttt gccaccgacg cccggctgaa aattgaggtt
    25801 gtcgagtttt acgacgacca gagcggctat gagcgcggac tgacgctgcc gcttcgccat
    25861 ccttcagggc tgtttgatgg tgaaacggaa gccgtatggg ggctgaacac cgcatacagc
    25921 gtggtggaaa aaagcgtcac cacgcgggac tacaactacc gcaccgccac agcagagatg
    25981 atgacagagc agcatgacgc caccgggggc gataacacca cctatggcga agcctaccac
    26041 tacgctgaca acttcctgca aaaaggcgac aaggaggcag cggaaagcgg cgcgttttat
    26101 gcccgtatcc gtcacgaacg ctacctgaat gaacaggcca ttctgcaggg ccagagcacc
    26161 agttcactgc tgatgccggg gctggaaatc agggttcagg gcgacgacgc gcctgcggta
    26221 ttccgcaagg gcgtgctgat taccggcgtg acggccagcg cggcccgtga ccgcagctat
    26281 gaactgacgt tcacggcgat accctactca gaacgctacg gctatcgccc ggcgcttatc
    26341 ccccgccccg taatggccgg aacactcccg gcgcgggtca ccagtacggt gaagaacgac
    26401 atctacgccc atatcgataa ggacgggcgc taccgggtca atctcgactt tgaccgtgat
    26461 acgtggaagc cgggctacga aagcctgtgg gtacgccagt cgcgcccgta tgcgggagac
    26521 acctacggcc tgcacctgcc gctgctggcg ggaacggaag tcagcatcgc ctttgaggaa
    26581 ggcaacccgg accgaccgta tatcgccggg gtaaaacacg actcggcaca caccgaccat
    26641 gtgaccattc agaactacaa gcgcaacgtg ctgcgcaccc cggcgaacaa caaaatccgc
    26701 ctcgatgatg agcgcggcaa agagcatatc aaggtcagta ccgagtacgg cggcaagagc
    26761 cagttgaatc tgggtcatct ggtggatgcc ggaaaacaac agcgcgggga aggctttgaa
    26821 ctgcgtaccg atatgtgggg cgcggtgcgg gcgaagaaag ggatttttat cagcgccgat
    26881 gcgcaggata aggcgcaggg gcaggtgcgc gaaatggctc cggcgatggc aatactggat
    26941 ggcgcacagt cacagatgca gtcgctgtcc actgatgcgc agacgacgaa tgccgatccg
    27001 gcagatctgt catcgcagat agcgctgttg cagcaaagtg taaaagatct gacgcaggcc
    27061 gtgatcctgc taagtgcacc caagggcgtg gcgatcgcca gcggcgagca cctgcaactg
    27121 gcggcgtcga agaatgtgat agcgaacgca ggcaacaacg cagatatcgg cgtagtaaaa
    27181 aatatgttta tcggtgtggg tcaggcgctg agcgtgtttg tgcgtaaagc gggcatcaag
    27241 ctgtttgcca ataaaggggc ggtctcggta caggcacaga acgatctgat ggaactgttg
    27301 gcacagaaat cgatagagat caccagtacg gaagatgaga tcaagatcac cgcgaagaag
    27361 aaaatcaccc ttaacggcgg tggcagctac atccggctgg atgcctgcgg gatagaagcg
    27421 ggaacgccgg gagaatataa cgttaaggcc ggatattacg ggcgtaaacc aaaggcgaag
    27481 ctgacgccgg agctgatggc atttccggtg attaagtctg aggattttaa tcagagcttt
    27541 attttgctgg atgaaaatac tgggcaacca cttattaatt ggccctatga actagaactg
    27601 gaaagtgggc taaaaatgag tgggattact gatgaaaatg gaaatacaga actaatatcc
    27661 tctgataagg aggaggtcgt taatattagt gtgtttgaac ccgatgagtt tttagacgat
    27721 gacattaatt aagacatata gcgagaatat aacatggcag ataaatccga aaagaaaaaa
    27781 gaaaccacaa ataaatcagc aaaaaaaaca ctaacgaaga agagcgaaaa attaactcca
    27841 gcaaaagaca ccgcaccaaa acaagtgaat gtagatccag ctataagatt gaaacaaatt
    27901 cgtcaacttg tccgtaataa taataaatcc atcatgggag aggattatat tatttgtcaa
    27961 atctataaag aatctagatt taagcaattt gcaggtaaga ataaacataa tgcgaaaggc
    28021 ttaatgcaaa tgcaacgaaa tgcagtgagg caggttttta aatatcgaca acaaaaaaat
    28081 aaaaggaaga atgacgactg ataaagagac aaatgaagct ttcgctaatg ctgatacatt
    28141 ttataaatct gataaaatat ttgatgaaaa agaaaatatt aagattggca cagagtattt
    28201 gcaatactgg atagataaag aagcaacaat tgaggaggca tatcgtggaa cagatgaagc
    28261 atactattct gttataacac cttgcgctga aaaactggct aaagatcctg ataacataca
    28321 aatactaatg gagggaatag gaagatagta tgcgcattat gttaatatta ctttgttttt
    28381 ttctttcccc tgtttttcag gcgataagat taaaatcgtg aaaacctgtc aactgaaaaa
    28441 aaatgaaagt gtacagttgc tttcccttag aaccattgac ggtgatacat tatatctaaa
    28501 attcagaaat gttatcaccg aggccttttt agatactcca actaaaagtt atgatttttt
    28561 tggagatgtg gttctttctc agtgcataaa tggttctctt atctttgcgc tggaatatgg
    28621 ttctccatat ataaaaggtt gtctggtaac tggttgggaa aatgggttta aaggtaagaa
    28681 ctctcctgaa ggcttttgtt tcgcagagag aaatattccc gaagcggtat ggtttggaaa
    28741 aaacaatatt ttaattatca tccgaaacca aatggatgtt gggacatgga aagggaaata
    28801 tattatctat gataatgcga aaaagtcagg agaaagagca tactcatcag ataatctacc
    28861 atcaaaagat ggttacataa tttttgcatt aaataaataa aatgactcta tagataactt
    28921 taaatttcac ttggttatta acttctgtcc gataatgcaa aataaaggga tgtactgatg
    28981 cctgtccgac ttaataccat tcccgacgca gaatcctatc ccgcaccgcc agcccgcatt
    29041 aaatggctgt cagccttatt actgatgctg tctgttggtg tggcaataac ctcattattc
    29101 gccagcgatg aactggcaaa aaacggcccg catttctggg ggctggcctg cggtgtgcct
    29161 gcctttatct ggtcgctggt tgcttcagtt cgctggttgt tttttatcac gcagtacatt
    29221 cgggctgatg cctggaataa gcgccgggaa gaggtgattt tgcaggaaac gcgacgggga
    29281 cgacgggcgt tgcagatact tagtttttcc gtacaaaccg cgctgaatgt tgattccgtt
    29341 gcagaaacta caacagcatt tctggcgcgt cagcaggtgt tgaatactta cgctgatttg
    29401 cgcggagaag atactgtgcg tcgcagtgtc attcccgtat taaccaataa accggttacc
    29461 gggcgtttat ccgaaattat ttcccgcctt tttattgata tcagaccgca attgtttctg
    29521 ctgccccctg attttcatat caacgtacta ctggaaattg atgcgccgct ttcaggggcc
    29581 tccgtgagaa ctatctggca ggacgaatgg caaaaagccg gactcccgga agcccgtctg
    29641 ttttctgccc cggagccggg gctggccgcc gtggacgact ggctggacaa ctttattcag
    29701 gaaaaggccg tactgctggt gatttccgtg tatctggaac cgaagactct ggaacggacg
    29761 gcagaatcct ccaccgcgct gttgctggca aaccggttga cgcaaaccgc gctgacgccg
    29821 ctggccctgc tgcatcgtcc tgaacgtatc acagatacag agatgatggc gtcaggcatt
    29881 gcacaggcgc tgga
//
LOCUS       DAHBHC010000063        29862 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHBHC010000063 DAHBHC010000000
VERSION     DAHBHC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.063"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(87..638)
                     /locus_tag="M2A35_003325"
                     /pseudo
     CDS             complement(87..638)
                     /locus_tag="M2A35_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(662..1207)
                     /locus_tag="M2A35_003326"
     CDS             complement(662..1207)
                     /locus_tag="M2A35_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7199582.1"
                     /translation="MLHLKNITAGNPRTAEQYQLTKQHGITWLFSEDDRNWYEELKNF
                     ASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITVPGYWFYRDDKFV
                     FDYKLKAEDERDALLQRASIMTSEWEKDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     TIADKSSYNAIEWPVSPEASS"
     gene            complement(1210..2763)
                     /locus_tag="M2A35_003327"
     CDS             complement(1210..2763)
                     /locus_tag="M2A35_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7199583.1"
                     /translation="MILGFGNNVVSALAGDITTIQTDIPVMPGTGAKFAKLLSADFEN
                     KSNGQRVYAKITLTDNKESAFEICHLVSVSGDVLKVIRGQEGTTAKGWSLNDVVANFA
                     TRGSENHFVQIAQLQSGHYIAGVAGGTANALTLELPATFFVNGGTDWTLRTPIIVFPV
                     QNNTNAATLQLTLGGKVLGTFPLYKGNKSELVANDIIKGIPLICLLDSEKSYFSVINP
                     GNIYSDFDLRYVKKSGDLMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQ
                     GENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIAFGDND
                     TGIKQNGDGILDVYANGQHVFRFQNGVAIALKNIQAGDAKKFTLSSANNSTKNATFNL
                     WGNSSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQMTPSNYGNFDARYQQRNGGVQ
                     DVRYGSEMYYNPGGNEITWTFRSPSGHGLSGIYVQDTGRGSADNIGGVYYRPLQKLIN
                     GTWYNVASV"
     gene            complement(2760..3386)
                     /locus_tag="M2A35_003328"
     CDS             complement(2760..3386)
                     /locus_tag="M2A35_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199584.1"
                     /translation="MQYLTEKILPAYPFVQYRDDPNVVAFFDAYNEIAQEYLDSLNNL
                     ALPCWTSESITGQLLDWIALGIYGVERPLLQVSEEAIARGAYDTIEYNTIPYAAMRNY
                     VPGQASYVPDDYFKRILTWNFYKADGSHFCIDWLKRRVARFIHGKNGIDPPLQHTFDV
                     SVTVSDSVFSIQIPEYGDGIGYFLKDAIDQKYVKLPFIYSYATTVIQK"
     gene            complement(3370..4596)
                     /locus_tag="M2A35_003329"
     CDS             complement(3370..4596)
                     /locus_tag="M2A35_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCB7199585.1"
                     /translation="MSEIPITMTSAGAQPTPPNDLLANLITRVAEKVPGYTANLPAGL
                     ITDLASTAVGALALIDQARVDLINSVSPYGANIPLLMQLGNIYGAQKGLSTNTAVYVV
                     FEALPGFGIPKGFVVGDGNYQYAVSRDTVVPESGQTEPVYCVATTSGSWAVPEGTVTQ
                     VITSVPKDQPVKCTNLTAGMPGQEAQTWASYRAEVMESGMFGVQGTPDCFKAMLKSVS
                     GVRENLISFRQSSLGKWVAVVGGGDPYDVAYAIYKSVPDISKLTNDVSNPSGAAVEKR
                     TVSITVSPDVYQVPFVIPSSQNVMVLITWNTVSDDYVDPAGIAMAVQQNVADYINSIE
                     VGHPINLLRIQDIFTSSVRLLVDATLISTISVSIGINGHIVLPAKDTSLVYGDTYSYF
                     STVASQVQVNKYAISD"
     gene            complement(4639..5385)
                     /locus_tag="M2A35_003330"
     CDS             complement(4639..5385)
                     /locus_tag="M2A35_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199586.1"
                     /translation="MIKFKLFVVLAFLLQIGTANAVSFDCSKAKSYSEKLICSNSELS
                     ELDDKLNSLYQKVKLESADKKAFRKIVSDLWNEREKCKTESCVRQWYSNAFREYSSLN
                     EINENIINESKNYAEWYIDGALIILPPLEGVSKNRFLMYGDDGTIAIFIASKSKGNGF
                     CDNSVQIDGPLEYPPILINGEYYKTYKSCLYGNGFIVPKTEIGKAVLKELVMKMKPIK
                     ISFGDGDEFNYPPSNVKRMLERVEKMKQAK"
     gene            5706..6263
                     /locus_tag="M2A35_003331"
     CDS             5706..6263
                     /locus_tag="M2A35_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HCB7199587.1"
                     /translation="MTSQLIPVFNGIVSNETTLLCNARDLHTFLEVGKRFATWITERI
                     GEYEFIENQDYIAISQKREIGHGRGKKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLREDTTSTQSPLNVDIIMRVRNGSVTHIEHHKSGSVITTEWAIHLLRKSGWIVMP
                     RDELLNTPLAQLMPENLPQTGTCVR"
     gene            complement(6239..6586)
                     /locus_tag="M2A35_003332"
     CDS             complement(6239..6586)
                     /locus_tag="M2A35_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199588.1"
                     /translation="MRTWGRVTDANGNKKWVAVESDANGDFSYGWLTTLIQTLKLGLG
                     ESPFYANYGIPAQQCIVQQIYPDYYVNMVQQQFAGYFASLAISKVDGAENPTYNIDVV
                     FFNGTSYRTQVPV"
     gene            complement(6836..7549)
                     /locus_tag="M2A35_003333"
     CDS             complement(6836..7549)
                     /locus_tag="M2A35_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCB7199589.1"
                     /translation="MSNAQKLPFLRTLSEMMTSSGNQQAELKGRELPCHVVDVSGQIV
                     TVQFDMLPEGINFPQITIPVATFPYIRYPIQPGDRGVTIAADVSLRGVSGLGTGMATL
                     SYSMSLTPLFFVPLANKDWSDEDPQKIVLYGPDGAILKTEDGSSSVTVAPEEIRQKSK
                     AVYLEAEDIFLNGKIHLNGPIVQDKAQMKDTTASLIGPLKVEKDAVINGVSASSHSHD
                     VTGVQSGGSTITSKKPNPG"
     gene            complement(7549..8556)
                     /locus_tag="M2A35_003334"
     CDS             complement(7549..8556)
                     /locus_tag="M2A35_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199590.1"
                     /translation="MRYYRLEIINPKTGKPPVDCNGKPIGPFDTSETPGCGLHVEFDF
                     EVTGLDVVCSGTMLTIYGLPIDMLKQSVSLQGCLVRMKAGFVEGLPLANPQQQGEVIY
                     GEIYLAYANWIGTNQTLNLVINPTVRKTDDGKPFSIEGEGLKGEKVGDVISRALQKAF
                     PNKLIDCTVSDSLVLPEPWKGTYDNIGSLAMVVRSASIAMMRNERYSGIAISILSDRI
                     RIYDNASAKWGEPKTIHAHELVGQPTWIAPFTVSFKCPLRGDIRCGDVIELPEGLYSG
                     AASIVMANTTAPSVIAKNSTTFTGKFLVKSVRHIGSYLTADGDAWVTVFEAYAENWAR
                     V"
     gene            complement(8556..8822)
                     /locus_tag="M2A35_003335"
     CDS             complement(8556..8822)
                     /locus_tag="M2A35_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199591.1"
                     /translation="MTTLIPFKPDGRGPFQFTARIGKYETFARVPFNLYANRYYLELK
                     DSSGDVIVYTPLIASPDNYDINLALPCSPGKLVFRESSNQFEVS"
     gene            complement(8819..9487)
                     /locus_tag="M2A35_003336"
     CDS             complement(8819..9487)
                     /locus_tag="M2A35_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199592.1"
                     /translation="MSLNYFGQAFKLAFEVSPILLVDGIASKIPGGVMPIAVLTEGLS
                     IANGLLHGEIRTRSMAAFTPMAGTTLVQQDICNLNFYNQVTAANATVRKPNRVVMQMI
                     RPASTEDGGYTTKGMTFTALKMALDMHNQYGGCYTVLTPSFIYTRCLMRSFIDTSGFS
                     EQNKQVQHTWQIEFEQPLSSVEQTVKTLASVLDKFDKGMPSDGPLSWSGIKNQVVQEF
                     GIGL"
     gene            complement(9491..11566)
                     /locus_tag="M2A35_003337"
     CDS             complement(9491..11566)
                     /locus_tag="M2A35_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCB7199593.1"
                     /translation="MAGNQMPVLTLDVNEEHLRRLEAIFEKYRNGLMIGPAGTPLKIP
                     SNTGQGGGAWQTTTGGEANQAPRKPSSPAPVPAASSDGRLRDEKGRFVGSGKTADSLV
                     SNYKGRGETMFDKYLSGLGKNAKQTLKTYKQINSTLQTTTSRLNNLFKTTVSWGTKLA
                     VMGVAGPFGFGMMSRSVVEKQRNADELTATPGELKAAESTYSPYFSGVGNLLNTLAAA
                     QNDTQHPAYNGLIGLGINPKKGAAENLPVLLERVAALAKEYEGTGLTQGMLRGRGLGW
                     VNFGLANQLVKYQDKIPELNKEFSLRAAQNDSLLTSAHTSKYQNLTSNLENNWDRLTS
                     GFQGAMSGNSEQLIGISNGVTNAALNFMNGENFKRILTDVETGLDKLGKYVNGPDFNN
                     DLNNFAENVAKVTKALSGFVGFAVEHPWLFGAAVLAGPSRVGAVAATTTGVAARVVGG
                     SLLGATAGTVAGLAIPTNNTPTTSEEMKGLEGRFNFDYFNEVQEWQKNNPGKVWPGGL
                     QKYSNNVNRSAYLSRGIRNNNPGNLNFAGQKGATLESGPNARFASFPTMLEGIAALDR
                     QVMLYLKRGKNTIDQIIDIYAPSSDGNNTSSYKSYLSQYTGLGVKEKIDGSNFELMRK
                     LIQGIINHENGAAARAVSGDDVMRALAMNRGNVYSPNNASQVIRLEVQQKPGSDILAQ
                     LAGMQQIPG"
     gene            complement(11631..12248)
                     /locus_tag="M2A35_003338"
     CDS             complement(11631..12248)
                     /locus_tag="M2A35_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199594.1"
                     /translation="MKISRNLNLIIPVRTEKGNGWIHATPISKEVFKEHFFILSKTFS
                     AIFSEGLGVVAGPRIAFLMLERISRDSNAWEGDKGVRNTLVNEVIRLANLVYPVEGKG
                     YDTIPLDMALEREIIDLDEVAGELIFFTCVSSINSPEQAKGTMDVVNGIWSTQCSSLN
                     LTEWIASLLTLKSAASSGATANTSSATSSTTQPEPDSETSVQILV"
     gene            complement(12245..12676)
                     /locus_tag="M2A35_003339"
     CDS             complement(12245..12676)
                     /locus_tag="M2A35_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199595.1"
                     /translation="MIPVGTLNRVRASVKFTSHSELNVSASFLAKEGVELSFQGNITE
                     FLPAMTGAVQSPQPYMILQARVHLLRSQALGKQFKAQWEKNATIGDAKVYSDSTVFGD
                     FDIYNTAITNVQDMTFAGGEPGVAITITGTYYINSEMWDLV"
     gene            complement(12700..14037)
                     /locus_tag="M2A35_003340"
     CDS             complement(12700..14037)
                     /locus_tag="M2A35_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199596.1"
                     /translation="MGFNIVTVNVSQTIGAIPSNLQQMAAILSFGSTTHEPGKPVLLT
                     RNQDINDLVKNPIAALSAAAAGKSAANVTVTMTLPEGSNIRRENSSEVKIVVSGCSPD
                     AWNGEYTATVTDEKTLTWTIADSQLSGSPVTLGQFSIVGSENLVTAVNTFFAQGNSVG
                     IYLLELGVQKGGVSKEIAALKAYMEDPLLRFYAYLVPQPWDGDAEFISLAKLHTANEA
                     MQYFFVLTKTPDDTNYVSPYAGIKSVIATADDTYPATNAAAAVMWNYVSASPSEINKV
                     PPMAFRYLQAVNAHKGKNSILATMTKQNINYVDTGAEGGISNTILVKGVTSDGNDMTY
                     WYSVDWVQINVDMQLANTVINGSNNPINPLYYNQDGIDRLQQVAQAVFNTGVSYGLVN
                     GQPVVDAVPFRQYINTNPNDYGIGRYAGLSASYTPMRGFVEIIFNINVTMQLS"
     gene            complement(14037..14981)
                     /locus_tag="M2A35_003341"
     CDS             complement(14037..14981)
                     /locus_tag="M2A35_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199597.1"
                     /translation="MPSLEELAEQHSSQLSSVLKSAVETISSDQEITFRLYVRQVLPL
                     DGFVYWVNAEIISCDELCRLNIESPTRLKIKGSLHRQVIAIQDESVSKDVNNIIFTPV
                     QQVDDFNVENPDAIYLGEYGGVQFAFSRMESRYQQSGIFHYRGMAILPTMRSQIIDCE
                     EDISDEQIISNSIPIWLQMKDAATVYPSYLVPQNLRPPYIAVDVRNSIPLQVAPVVFG
                     GERFQLVQDSVRLTLYGFSNKMALDLVDSVVNRALEEEKFGVTNIPVVQDAKSGQVEI
                     NALAKKKIVDFDVNYYQSTAREISRQLIEKVICKYEVK"
     gene            complement(14968..15408)
                     /locus_tag="M2A35_003342"
     CDS             complement(14968..15408)
                     /locus_tag="M2A35_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199598.1"
                     /translation="MKLCFGVVDQPYDYGDEPGKTTFDVACDLEERYEIFTHFWEMHK
                     DEIIQEAGEMVAFQLIRHLKNKVPLPPVQVMGDTRGIFHRFLEAEEMAGMTINGNPVP
                     TQAALLGVNSRLKDKYTGERRPSFIDGGLFKGSFIAWIDNNAES"
     gene            complement(15405..15845)
                     /locus_tag="M2A35_003343"
     CDS             complement(15405..15845)
                     /locus_tag="M2A35_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199599.1"
                     /translation="MTLRPSLEGFIRFVRDDMKVPVHAIADDDPTLECCFQSAMELIP
                     HDQGLERLPIIYVRTVYNAAASFLLNFAPGSWFADLRKKLNLGKLATGLVSAAADQGT
                     SGSITISDALSNLSLLDLQMLQDPYGRQVVAVLMQMGTVWGYTP"
     gene            complement(15845..16315)
                     /locus_tag="M2A35_003344"
     CDS             complement(15845..16315)
                     /locus_tag="M2A35_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199600.1"
                     /translation="MKLYIANCSRQPHTFNYKLPEKMQSFGVTIPSGRQHMIENQSDI
                     IDHIIRQHEPYGFQRCDKVDKNFSGICYSIDKPVSVGRIEDCAEQKTENLESMSEEIL
                     AASAVSLNNAVDQAVIQSGEKPQPGGIEMEITGEAVNTEQENPPSTKRNIKVKK"
     gene            complement(16373..17401)
                     /locus_tag="M2A35_003345"
     CDS             complement(16373..17401)
                     /locus_tag="M2A35_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199601.1"
                     /translation="MSAFTPATTIVSPSMVLPEMIVQQSMASGAFEVLAGGAPAVKIS
                     SSDLMVYQKYLRMTSQAQVSQSLPGQLPSSSISGGYDGMMTYRISSRSQYSYLDTDAA
                     DRWGYSLIEGLRLANRQGHAQMLRNMLLYGVNAANNEGITNSPNAVTLNLGNDSKGND
                     SYTTWDSGEMAKFMLGLIADQKTRMLLLGQPLTTVILSPQRFMKALEWTGIVELTSYQ
                     RPGGGTGTVGTMVKDVADKATGDDIIFCQDDTLIGKGAGGNDLIIVTNPTIEVPEARH
                     AINTNIFSTLVPNQQAVNVMFCDMAAPTEIPSPMPDGGLTTLYTMRATPGWNFRPEGI
                     TLLSAKYA"
     gene            complement(17416..18033)
                     /locus_tag="M2A35_003346"
     CDS             complement(17416..18033)
                     /locus_tag="M2A35_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199602.1"
                     /translation="MADINFHPFKNRGAFGGLFNVESRGLMQGDAQDDPAIRLQLCSG
                     RLDSKITEPVWGGVGVIECIAPAKDSVNGAVIKQATTDACNAFTVFNQAFHGITTPDN
                     PVPLYLAGGFVHYYRVGSGARIPLPVSAEVVALADGNNTVAASGFVWDLTKNVVDVYS
                     GTPGANPKVDIKLLMVSVDGNLTVKKEDGGNVVWEIGKPCGLFLI"
     gene            complement(18026..19348)
                     /locus_tag="M2A35_003347"
     CDS             complement(18026..19348)
                     /locus_tag="M2A35_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCB7199603.1"
                     /translation="MSLTELEVAERIRDGTVPSPVKFSNMWLVNLRITGTGLAYRAGL
                     KEHVWRDPKLYLSEEFLRRCNGLPVIANHPDDAVLTEEDFKSRIVGSVMLPYIRGDEV
                     WAVCRVYLQSIVEEITEGDVSTSPSVVFNSTSGNVEVQEGDTNFLIEGVPFLVDHIAL
                     VTKAHGSLGVWDKDRIPAGVEVTNTGEIEMEKEELQALLQGVVSDALKGINQKIDGVV
                     TRMDSLEQRDKARADAEAQAKKEAEEKAKADEAAEEQRKADEAAAKEAEEKAKADEAA
                     AKDAEEKAKADSEAEEQRKADEEAEKERNDSALAEAQAKADSAFSACGKNAPAPFSGE
                     NALDYRKRALIAMQKHSPAHKDVNIRAIADSATLAVLEDAIFSAARQSIEKEMMSTQG
                     QLHKRIRNDEAGRRITEYQGDPNVWLSAFKIPGRRLAKINTQGSLNNG"
     gene            complement(19329..20162)
                     /locus_tag="M2A35_003348"
     CDS             complement(19329..20162)
                     /locus_tag="M2A35_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199604.1"
                     /translation="MAISSRAVRKKKRIRTLYEVLTDAVNYYVNHGWDSEKSLLEWCR
                     KLRVAAQRETPDDTVARKHLTAIYSRLVIDGGALRDQPPDGPKKITVEKLKPEFRKEL
                     DRRIFASANLIKLNREQAIEKTIQRFQGWVTSIPPDGVSEIDRREVKAGFQKSVKDMD
                     FISRRVAIDQGHKLASNVKYLLAVQSGAIALRWHSNWRRPGYKYRQDHKERDEKIYLL
                     RDSWALEQGLIKPVYGFYDEITAAGEEVYCSCDALPIYAPQKLPDEFLTEKGKREFNR
                     A"
     gene            complement(20107..21543)
                     /locus_tag="M2A35_003349"
     CDS             complement(20107..21543)
                     /locus_tag="M2A35_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCB7199605.1"
                     /translation="MAEIEISVNLSSSLMRILEAEEIQPGTDIGYELCKLLWQYHPLG
                     GKLVEKPILMAMCKPRQYNVETDPDERVVRRFQEVWERMKVNEKIKNLFFLSRCYGAA
                     AIGVGTDSVSCREPLPTFGLTEEDVYINAWDPLNASGSMVTDQNPNSPFFQEANKKLK
                     IGGKDWHPSRTLKIFNGTPIYLEFQNSSFGFTGRSVFQRVLYSLKSYINTMEANDLVS
                     QKAGVLVAKVVQYGSKIDGIMAAATGRKRENVKAAKNKGVLSIGKDEDVSSLNLQNID
                     GALNTSRDNIIADIAAGSDVPAIIIKEEAFSNGFGEGKEDSKAISQYIDGVRQQIEPV
                     MDYFERLVQYIAWNEEFYQSLKNDYPDIITDDYKTTFYQWRREFTATWQELVEESPDK
                     RRESDSKVIQQAIALFSAVSPQVDPENRAALAEWLASLVNATQTYGEAPLIIDVDALA
                     NYEPPKQETPDGNFQPGGEEEETDQDAV"
     gene            complement(21563..22891)
                     /locus_tag="M2A35_003350"
     CDS             complement(21563..22891)
                     /locus_tag="M2A35_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCB7199606.1"
                     /translation="MSIEIYLPKPHEGQIAAWTAAIEERFHAVCCGRRWGKTVMLVNI
                     ATSFATRKFAIPTTGQLIAGRVGIFTAQYRQYQEIWDEISAVLQPLILSQSKNEKRII
                     LRNGGRLDFWVTDNNKLAGRGRKYHAVLIDEAAFTKSPEMLEEIWPRAIRPTLVDYRG
                     CAWVFSTPNGIDESNFFYAICHDESLGFVMHHAPTSSNPYIPKEELEETEKKSDPRVW
                     QQEYLAEFVDWSKDALLDVDKLLVDGQPIEMPPYCDMIFAVMDTALKGGTENDGTGVV
                     YFCYESTYSDEPKLTIIDWDVTQIKASLLPEYIPGVYDNLERLAKLCRPRLGSQGIFM
                     EDAAMGAILNQKAETEGWDMTPIKSALTSKGKDERAVMASSYHYQGMCKIVREAYDKT
                     VSFKRTTANHLIKQIAGFHLADKDAHKRADDLFDCYTYGLIIAHGNYAEL"
     gene            complement(22881..23972)
                     /locus_tag="M2A35_003351"
     CDS             complement(22881..23972)
                     /locus_tag="M2A35_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCB7199607.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     PSKNAQTEVRKTAQKSAQKKFAQTAQKRNGKSQKKKSVSDARLNEGDAEEISFCPDEF
                     GISDQQAKFAMLVAQGKNLIEAYRLAGYEGTGNVASAAASRLLRNVKVYRAISWFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGAK
                     LGKFGVEILLEDKAAARRELIKLIMATKGSSAGDATDSRNELELEGLRLRNEKLRTEI
                     ENLKKGVGGENNEIIIHNSLPMPGVDNVD"
     gene            complement(23976..24227)
                     /locus_tag="M2A35_003352"
     CDS             complement(23976..24227)
                     /locus_tag="M2A35_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199608.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTNFYAS
                     IRKGRAKGEAEIRAALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(24163..25095)
                     /locus_tag="M2A35_003353"
     CDS             complement(24163..25095)
                     /locus_tag="M2A35_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199609.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKVFIVVDDEDKTR
                     HQYIAEFGEQVLVFSKADIASRFDEADNFGDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNFGRRGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(25088..25879)
                     /locus_tag="M2A35_003354"
     CDS             complement(25088..25879)
                     /locus_tag="M2A35_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCB7199610.1"
                     /translation="MSEKLKIVYRPLQKLSPYAHNARTHSPEQVAQLEESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEVLKMDSVPVIVLSGLTDDQKKAYRLADNRLPLNAGWD
                     EELLRVELSDLINADFDVSLTGFSPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GDKPDISELYDDTKTQELVSRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYVHAP
                     AEIQNLFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            complement(25998..26186)
                     /locus_tag="M2A35_003355"
     CDS             complement(25998..26186)
                     /locus_tag="M2A35_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199611.1"
                     /translation="MESPIMKYFNYEHLPTSLKEVSKPIGDLARVMDESLPDSPEKSA
                     GLRKLLEAKDALVRAKLG"
     gene            complement(26219..26374)
                     /locus_tag="M2A35_003356"
     CDS             complement(26219..26374)
                     /locus_tag="M2A35_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199612.1"
                     /translation="MPRTAAKWALVAILVPFLLVGCVSLDKARQLFDTASQVCEIVDG
                     VRQCMQN"
     gene            complement(26403..26516)
                     /locus_tag="M2A35_003357"
     CDS             complement(26403..26516)
                     /locus_tag="M2A35_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199613.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRLVAVAVLIWAVRWW"
     gene            complement(26647..26928)
                     /locus_tag="M2A35_003358"
     CDS             complement(26647..26928)
                     /locus_tag="M2A35_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199614.1"
                     /translation="MYALKKITVTEDGRQVEEVHVLGDMYRLEFYPRDTHLAAKIEYC
                     LGGDIPCIPVEKEDEAYITTLTGDTVRCICRGDSKARNEIARCRTQGSK"
     gene            complement(26999..27376)
                     /locus_tag="M2A35_003359"
     CDS             complement(26999..27376)
                     /locus_tag="M2A35_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCB7199615.1"
                     /translation="MSGKFRFSRRSEKNLEGVKPQLVAVVRRALELTEVDFGVTEGLR
                     TKERQKQLVAEGKSQTMNSRHLTGDAVDVVAYIGSQVSWDWPLYEKIAQAFKQAAAEL
                     GTAIEWGGDWKTLKDGPHFQLKR"
     gene            complement(27379..27654)
                     /locus_tag="M2A35_003360"
     CDS             complement(27379..27654)
                     /locus_tag="M2A35_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCB7199616.1"
                     /translation="MLSNLPGLLNVALCTVIVLTLFFYRRRDSRHKPLMSWLAWLLML
                     LYAFAPLSYLCGRPLAANWLAVGLNLLFCVLVIRARGNVSKILSFRR"
     gene            complement(27644..28036)
                     /locus_tag="M2A35_003361"
     CDS             complement(27644..28036)
                     /locus_tag="M2A35_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCB7199617.1"
                     /translation="MSEPLSGSGTAAALGGATVFGLFTGTDFGIVFGAFAGALFVATM
                     PQKISAWRVAAHFLVSFIVGVLGARVLSAWIASKTGYDGTSADALCAVLVAVVSVKIL
                     SFIHQQDIASLVSGLFSRLRGGGGGNVK"
     gene            complement(28128..28280)
                     /locus_tag="M2A35_003362"
     CDS             complement(28128..28280)
                     /locus_tag="M2A35_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCB7199618.1"
                     /translation="MKSARLVLAAILLFLVVVVDFTGRLMSVLADGVLVAMALAVLRP
                     LLRKSE"
     gene            complement(28277..28702)
                     /locus_tag="M2A35_003363"
     CDS             complement(28277..28702)
                     /locus_tag="M2A35_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCB7199619.1"
                     /translation="MFGIFKKKTRKAITEVKKMENRDAVEATVWGAYSIAYADDTCDA
                     KEVAVLEKTIAALPAFAPFSGEIAQMSANIRARYEASPRSANAEALRQLADVAGTDDA
                     VNVLCLCLDIADQDGIGPDEEAQLKKIAQALQLPLEQYL"
     gene            complement(29383..29458)
                     /locus_tag="M2A35_003364"
     tRNA            complement(29383..29458)
                     /locus_tag="M2A35_003364"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29423..29425),aa:Gly,seq:tcc)
     gene            complement(29462..29537)
                     /locus_tag="M2A35_003365"
     tRNA            complement(29462..29537)
                     /locus_tag="M2A35_003365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29502..29504),aa:Thr,seq:tgt)
BASE COUNT         7302 a         8103 c         6649 g         7808 t
ORIGIN      
        1 gtttctccct gcggcccttg cggtcctggt gtttttgcaa tctctttcgc ttcttccagc
       61 gctgtcagtg caccggactc gtgcaactcc gcgttttttg ctgcctgttc tgcacgatct
      121 gcatccagtg aaaccgaaga cacaatttgt tccacgtgct ttttatcttc ggcaacggat
      181 tctgcattct gctgtacgtt atccgccagc gtctcgcagt cgctcttaat ttgttgcgca
      241 tcagcgacat gttgcccggc ctgtcgttcg cttttcgctg ccgcttccgc gctctgctgc
      301 gcctgcgcca ccatttcctc aaagcgtttc actacatccg gctttaaatc accttcatca
      361 agggcagtca gaaagtcgtt cagcgtgccg ggctttgagt cgtcgtatac agcaatgtcg
      421 ccaacacagt actcgtcgca ccagtcctgt ttcagatata cgcaatattt tccggtctgc
      481 gccttaaaac agtactcgcc acagtttcct gtcaccacgt cggcaactgt gcgcatcacc
      541 acttcggagg tgtttacccg ggatttcaga ataatgtggc atccggacat ggggatgcct
      601 gcgccatcaa tcagcgcacc tgatatcact acagacatag tttttctcgc aataaattaa
      661 atcaggaaga ggcttccgga gagacgggcc attcaatggc gttatatgag cttttatcag
      721 cgatggtgct gaaatccatc gcctgcagcg atttcgcgta tatacggtac gctttcagtt
      781 tttccctgtc ttcgtcgctg attaatccca gcagcaggtc tttttcccat tcgctggtca
      841 tgatgctggc ccgttgtaac agggcatcgc gctcatcttc cgctttaagt ttgtagtcga
      901 agacaaattt atcgtcgcgg taaaaccagt aaccaggcac ggtaatacgg cggttagcgg
      961 taatatcagg aacttcaata acactagcgt tacggggttc aatacctgtc acatccttac
     1021 cgacccacac cacgcgcccg tctccggtgt aaaccatttt tatggtgtca ctggcaaaat
     1081 ttttcagctc ttcataccag tttctgtcat cctccgaaaa aagccaggtg ataccatgtt
     1141 gttttgtcag ctgatattgc tccgcggttc ttggattacc cgcagtaata ttttttaaat
     1201 gcaacattgt taaacactcg ccacgttata ccaggtgcca ttaatcagtt tctgaagcgg
     1261 tctgtaatac acaccaccaa tgttatctgc cgaacctctt ccggtatcct gcacataaat
     1321 accggataat ccgtggcctg aaggtgagcg aaacgtccag gttatctcgt tacctcccgg
     1381 gttgtaatac atttcggaac cataacgcac atcctgcacg ccgccatttc gctgctgata
     1441 acgggcatcg aaatttccat agtttgatgg ggtcatctgt ccgtttacag cgaatgtgat
     1501 actgccatcg gtatttctct ggctgtaaaa atgccagccg gaatcatcac caagctctgc
     1561 aactacaggt cgggatgaat tgccccataa attaaacgtt gcgtttttcg tggagttgtt
     1621 ggcgctggat aacgtgaatt ttttagcatc tccggcctga atatttttta acgctatcgc
     1681 cacaccattc tggaaacgaa atacatgctg tccattcgca taaacatcca gaatgccgtc
     1741 gccgttttgt tttatacctg tatcgttatc cccgaaagca attgagtttc cgcccagggc
     1801 attctgaacg ccgataccca gcgcaccatt cacctgagaa ccgccaccaa cagacacttt
     1861 atgcgacatg gatatttcac ccgtccgcag attaatagtg aacggtcgaa gtggaccaat
     1921 atcgccattc tcgccctgac cttcactggt agggataagg tgcaggcact cttccgaacg
     1981 acgaaaaatc agaccaaagg cttcgttgaa aatcctcagc gcattaacac cacggatttt
     2041 cagttccccg gtcatcaaat caccagattt ttttacatat cgcagatcaa aatctgaata
     2101 gatattgcca gggtttatca cgctgaaata gcttttctcg ctatcaagaa gacaaattaa
     2161 gggaatgcct ttaatgatat cgttcgctac cagctcggac ttgttcccct tataaagcgg
     2221 gaacgtacca agaacctttc cgcccagtgt taattgaagt gtcgcggcat tggtattgtt
     2281 ctgaacgggg aaaacgataa tcggggttcg tagcgtccag tctgtacctc cattaacaaa
     2341 aaacgtcgcg ggaagctcca gagtcagcgc gtttgcagtg ccgccagcaa ctcccgcaat
     2401 ataatgacca ctctgaagct gcgctatctg tacgaaatgg ttttccgaac cacgcgtggc
     2461 aaagttagcc acaacgtcat taagggacca acctttcgcg gttgttcctt cctgcccacg
     2521 aatgactttc aacacatcac cgcttaccga taccaggtga caaatctcaa atgcagactc
     2581 tttattatcg gtaagcgtaa tttttgcata gacacgttgc ccgttcgatt tattttcaaa
     2641 atcggcagaa agcaattttg caaatttggc ccctgtaccg ggcattaccg gaatatcagt
     2701 ctgaatcgtg gtgatatcac cagccagtgc tgaaacaacg ttattgccaa atccaagaat
     2761 catttttgaa tcaccgttgt tgcataggaa taaataaaag ggagttttac atatttctgg
     2821 tcaatggcat ctttcagaaa atagcctata ccatcgccat actctggtat ctgaatagaa
     2881 aaaacgctgt ccgatactgt cacactcaca tcaaaagtgt gctgcaacgg cgggtctatt
     2941 ccgttttttc catgaatgaa ccgcgccaca cgtcgcttta accagtcaat gcagaaatgc
     3001 gaaccgtcag ccttataaaa attccacgtt aatattcgtt tgaaataatc atcaggtaca
     3061 tacgatgcct gccccggaac gtaattccgc attgcggcat acgggatcgt attgtattca
     3121 atggtatcgt atgcgccgcg tgcaatagcc tcctcggaaa cctgtagtaa aggcctttca
     3181 acgccataaa tcccgagtgc aatccagtcc agcaattgcc cggttattga ttccgatgtc
     3241 cagcatggca atgccagatt gttgagtgaa tcgaggtatt cctgagcaat ttcattgtat
     3301 gcatcaaaga acgcaacaac attcggatca tctctgtact gcacaaatgg ataagcaggg
     3361 agaattttct cagtcagata ttgcatactt gttgacctga acctgtgatg ccaccgttga
     3421 aaaataggaa taggtatcgc cataaaccag gcttgtgtct ttcgccggaa gaacaatatg
     3481 gccgttaata ccaatgctca cactgattgt tgagatcaac gtcgcatcaa ccagcaacct
     3541 gacggaactg gtaaaaatat cctggatacg cagaagattt atcgggtgtc cgacttcaat
     3601 tgaattgatg taatcagcaa cgttttgctg cacagccata gcaatacccg ccggatcaac
     3661 ataatcatca gacaccgtgt tccaggtgat tagcaccatg acgttttgtg atgacgggat
     3721 aacgaacggc acctgataaa cgtccggcga aacggttatt gaaaccgtgc gtttttccac
     3781 tgccgcaccg gatggattgc taacatcgtt ggtcagtttc gaaatatccg gtacagattt
     3841 gtaaatcgca taagccacat catacggatc accgccacca acaaccgcaa cccatttccc
     3901 cagcgacgac tgccggaaag aaatcaggtt ttctcgcaca ccgcttactg atttgagcat
     3961 cgctttaaag caatccggcg ttccctgcac tccaaacatg ccggactcca tgacttcagc
     4021 gcggtaagat gcccacgttt gcgcctcctg acctggcatt cctgcggtaa ggttcgtgca
     4081 ttttacaggc tggtcttttg gtactgatgt aataacctgc gtcacggtcc cttccggtac
     4141 agcccatgag cctgacgttg tggccacaca gtaaactggc tcagtctgcc cgctttccgg
     4201 taccacagta tcgcgggaaa ccgcatactg gtagttaccg tctccaacca caaatccttt
     4261 agggatacca aaccccggca acgcttcaaa caccacgtat accgccgtat ttgtacttaa
     4321 tcccttctgt gctccatata tgtttccgag ttgcatcagt aacggaatat tcgcgccgta
     4381 tgggcttaca gagttaataa ggtccacccg cgcctggtct attaatgcca gcgcccctac
     4441 tgccgtgctg gcaaggtctg taataagtcc tgccggaagg ttggctgtat atccaggtac
     4501 tttttcagca actctggtga taagatttgc gagcaaatca ttgggtggcg taggctgcgc
     4561 acccgcactg gtcatagtaa ttggtatttc agacatatct accccataaa aaaaccgccg
     4621 aagcggtttt aagttaactt attttgcctg tttcattttt tccaccctct caagcattct
     4681 ttttacattt gatggaggat agttaaattc atcgccgtct ccaaaagaaa tttttatagg
     4741 cttcattttc ataaccaatt ctttcagaac cgctttacca atttcagttt ttggtactat
     4801 aaaaccattt ccatacaagc atgatttata agttttataa tattcaccat taatcaaaat
     4861 tggtggatac tctaatggcc catcaatttg cactgaatta tcgcaaaatc cattgccttt
     4921 agattttgaa gcaataaaaa tagctattgt cccatcatcg ccatacatca ggaatctatt
     4981 tttactaaca ccttccagtg gaggtaatat tatcagtgca ccatcaatat accattcagc
     5041 gtaattcttt gattcattga ttatgttctc attaatttca tttaaagatg aatattccct
     5101 gaatgcatta gaataccatt gtcgaacaca tgattctgtt ttgcacttct ctctttcatt
     5161 ccacaagtct gaaacaattt ttctaaaagc tttcttatca gcgctctcta actttacttt
     5221 ttgatataaa gaatttaatt tatcatcaag ttcagataat tcactgttgc tgcaaattaa
     5281 cttttcagaa tatgatttag ctttcgagca atcaaaagat accgcattcg cagttccgat
     5341 ttgtaataaa aaagcaagca ctacaaataa tttaaactta atcatccgta atctccttaa
     5401 aaacaggaga ttaaatttta cccattctta atgtccgttc aagttcatac tttacaaccg
     5461 cgcccagttg agtatgatta agcgcatgag gcgtcgaaac ctctttcacg cggcctagac
     5521 caaccccgtc agtgttggat tttttatgcc tacaattcag tgatggcaca atgtgcggtc
     5581 acatccccga tcaatgtcgg gagggcgacg aatacaacac ccgaaaggga aataagtccg
     5641 cggtttcgtg aagccgtttc gaacctcctg gcaccacaat ttcgaaaaaa tttcacgagg
     5701 tttctatgac cagccaatta attcccgtat tcaacggcat tgtttcaaac gaaactaccc
     5761 ttctctgcaa cgctcgcgat ctgcacacct ttctcgaagt aggtaaacgc tttgctactt
     5821 ggatcaccga gcgaatcggc gagtatgaat tcatagaaaa tcaggactac atagctattt
     5881 cccaaaaacg ggaaataggt cacggacgag ggaaaaagga ctaccacctc accctggaca
     5941 ctgccaaaga gctggcaatg gtcgagcgca acgaaaaagg acgccagatc cgccgatact
     6001 tcatcgagtg cgagaagaag ctgcgcgaag ataccaccag cacacaatcc cctctgaatg
     6061 ttgacatcat catgcgcgtc agaaacggtt ctgttaccca cattgagcac cacaaatcag
     6121 gctcagttat aaccacagaa tgggcaattc acttactacg caaaagtgga tggattgtca
     6181 tgccacgcga tgaacttctt aacacgccac tggcccaact gatgcccgaa aacttgcctc
     6241 aaaccggaac ctgcgtccgg taactggttc cgttaaaaaa cacaacatcg atgttatagg
     6301 tggggttctc tgccccatct acctttgaaa ttgccagcga tgcaaaatac ccggcaaact
     6361 gttgctgaac catgttcaca tagtagtccg ggtaaatttg ctgcacgatg cactgctgcg
     6421 ctggaatacc gtaattcgcg taaaacggcg actcccccaa ccccagcttt aacgtctgaa
     6481 tgagcgtcgt cagccagccg taggagaaat caccgttggc gtcagattct acagcaaccc
     6541 attttttgtt gccgttcgcg tcggtgacgc ggccccatgt tctcatcgtg ccctcaccac
     6601 catcggatct ggccaacaaa ataaccagac gccagaatca gaaagaccaa ccaaataagc
     6661 aaaaacttcc aggtggataa tttttcagcc ataactcgaa tctcccgaat cagtttgcta
     6721 aaatcaaaca cgacttctcc ttgccttatt caaggtcaga aacagaaaac cccgactggg
     6781 ccaacagtcg gggttttcgt tttatgggta gtatttaaag tgaaatgaac cggtattaac
     6841 ccggatttgg tttcttcgac gtgatcgtac tgccaccgct ttgaacacca gtcacatcgt
     6901 gactgtggct gctggcgctc acgccgttga taactgcatc tttctcgacc ttaagaggac
     6961 caatcagcga agctgttgta tccttcatct gggctttgtc ctggacgatt ggaccgttga
     7021 ggtgaatttt cccgttcagg aaaatatctt cggcctcgag gtaaacagct ttcgactttt
     7081 gcctgatttc ttccggtgcc accgttaccg agctactgcc gtcctctgtt ttgaggatcg
     7141 cgccatccgg accgtacaaa acgatttttt gcggatcttc gtcggaccag tccttgtttg
     7201 ccagtggcac gaaaaacagt ggagtgagcg acatcgagta agagagcgtc gccataccgg
     7261 ttcccaatcc ggacacaccg cgcagggata catcagcggc aattgttact cctcgatcac
     7321 ccggctgtat cgggtaacga atatacggga atgtggcgac agggattgtt atctgcggaa
     7381 agttgatccc ctctggcaac atatcaaact gaacggtcac tatctgccca ctgacatcga
     7441 caacatggca gggcaattca cggcctttaa gctcggcctg ctggttgcca gaactggtca
     7501 tcatctccga cagcgttcgg agaaacggta atttttgagc gtttgacatt acaccctcgc
     7561 ccagttctca gcatatgcct cgaataccgt cacccaggca tcgccatcgg ctgtcagata
     7621 tgaaccaatg tgtctgaccg atttcacaag aaatttcccg gtaaacgtgg tcgaattttt
     7681 tgcgataacg ctgggtgccg ttgtattagc catcacaatc gacgcagcgc ctgaatatag
     7741 cccctccggg agttcgatca catcgccgca tcgtatatct ccccgcaacg ggcatttaaa
     7801 actgacggta aaaggggcta tccaggtagg ctgaccgaca agttcatggg catgaattgt
     7861 ttttggctcg ccccactttg ccgatgcgtt atcgtagatt cgtattctgt cggaaagaat
     7921 actgatggcg attccgctat acctttcatt acgcatcatt gcgattgaag cactacggac
     7981 taccatcgcc aacgacccaa tattgtcgta ggttcccttc cacggctctg gtaaaacaag
     8041 actgtcactg actgtgcagt cgatcaattt attcggaaat gccttttgca acgcacggga
     8101 gattacatca ccaacctttt cccctttaag cccctctccc tcgatagaga acggtttacc
     8161 atcatctgtt ttgcgtacgg ttggatttat taccaggttt aaggtctggt tcgtgccgat
     8221 ccagttggca taggccagat aaatttcgcc ataaattacc tcaccctgtt gctgtggatt
     8281 tgccaaaggc aatccctcaa caaagcccgc tttcatacgg accagacacc cctgcaaact
     8341 cacgctttgc ttcagcatgt caattggtaa tccatagata gtcagcatcg tacccgagca
     8401 gactacatcc agaccagtta cttcaaagtc aaattcaaca tgcaacccac atcctggtgt
     8461 ttcactggta tcaaaaggcc caatgggttt tccattgcaa tccactggcg gctttcctgt
     8521 tttaggatta ataatttcca gtcggtaata acgcattacg aaacctcaaa ctgattagaa
     8581 ctttcacgaa aaacgagttt ccccggcgag caaggcagcg ccagattgat gtcgtaattg
     8641 tcaggtgacg cgatcaacgg tgtgtacaca atcacgtcgc cagaactgtc tttcagttcc
     8701 aggtagtaac gatttgcata cagattaaac ggaacgcggg cgaatgtttc atattttccg
     8761 attctggccg tgaactgaaa cggacctcgc ccgtccggtt tgaaaggaat taacgttgtc
     8821 ataagccaat accaaactcc tgcacgacct ggttcttaat acctgaccac gatagcggcc
     8881 cgtctgacgg catcccttta tcaaatttat ccagaacgct cgccagcgtt tttaccgttt
     8941 gttcgacaga cgataatggt tgctcaaact caatctgcca ggtgtgctga acctgcttgt
     9001 tctgctcaga gaaaccggat gtatcgataa acgaccgcat cagacagcgc gtgtagataa
     9061 acgagggagt cagaacggtg tagcaaccgc catactggtt atgcatatca agcgccattt
     9121 tcagcgccgt aaacgtcatc cccttagtgg tgtagccacc gtcctccgtt gatgccggac
     9181 ggatcatctg catgactacc cggttaggct tcctgacggt cgcattcgct gccgttacct
     9241 ggttatagaa attcaggttg caaatatcct gctggactaa cgttgtcccc gccatcgggg
     9301 taaatgccgc catcgagcgt gtgcgtatct cgccatgcag cagaccattc gcaatgctta
     9361 ggccttcggt caaaacagca atcggcatca ccccgccggg aattttcgac gctatgccgt
     9421 caaccaaaag aatgggcgag acttcaaacg ccagtttgaa agcctgtcca aagtaattaa
     9481 gtgacatggt ttaccccggt atttgttgca ttccggcgag ttgtgccagt atatctgaac
     9541 ctggtttttg ttgaacttcg agcctgatta cctgagaagc attatttggt gaatatacgt
     9601 tcccccggtt cattgccagc gcccgcatca catcatcgcc acttacagca cgagcggcgg
     9661 ccccattttc atggttaata atgccctgaa ttagctttct cattagctca aaattagaac
     9721 catcgatttt ctccttaaca cctaatccgg tgtactgaga gagatagctt ttataggacg
     9781 atgtgttatt tccatcagat gaaggggcat aaatatcaat aatctgatca atcgtatttt
     9841 tgccgcgttt cagataaagc atgacctgtc gatctaacgc agcaatgcct tcgagcatcg
     9901 tcgggaaact ggcaaaacga gcatttggcc ccgattccag ggtcgcccct ttttgtcctg
     9961 cgaaattaag gtttccggga ttgttattcc tgatccctct ggataaatat gctgacctgt
    10021 ttacattgtt cgagtatttc tgcaatcctc caggccagac cttgccagga ttgttttttt
    10081 gccactcctg cacttcgtta aaataatcaa agttgaaacg cccctccagc cctttcattt
    10141 cctcactggt ggtaggtgtg ttatttgtag gaatagccaa tcctgccact gttccggccg
    10201 tagccccaag aaggcttccg cctacaacac gggcggcaac tccggttgtt gtagctgcca
    10261 cagcaccaac tcttgatggc ccggcaagca ctgcggcccc aaaaagccag ggatgttcaa
    10321 ccgcaaaacc aacaaatccg ctaagtgcct tggtgacctt tgcaacgttt tcggcaaaat
    10381 tattcaggtc gttattaaaa tccgggccat taacgtactt accaagctta tccagacctg
    10441 tttcgacatc agtcagaatc cttttaaagt tctcaccatt catgaaattc agggcggcat
    10501 tcgttacgcc attagatatt ccaatcagtt gttcagaatt acccgacatc gctccctgaa
    10561 acccactggt aagtcgatcc cagttatttt ctaagttact ggtaagattc tgatatttac
    10621 ttgtatgtgc agaggtcagt aacgaatcat tttgtgctgc acgtaacgaa aactctttgt
    10681 tcagttcagg tattttgtcc tgatatttga ctaactggtt agcaagacca aaatttaccc
    10741 atccaagccc cctccctctt aacatccctt gagttaatcc tgttccctca tactcctttg
    10801 caagagcagc aactctttct aacaacacag ggagattttc tgctgcccct tttttaggat
    10861 ttatccctaa cccaattagc ccgttgtagg caggatgctg agtgtcattt tgcgcggctg
    10921 ccagtgtatt gagtaaatta ccaacaccgg aaaaataagg cgaataagtg ctttctgccg
    10981 cctttaactc tcctggtgtt gccgtcaatt catccgcatt tctctgtttc tctacaacac
    11041 tacgggacat catgccaaag ccgaacggcc cggcaacgcc cataaccgca agttttgtcc
    11101 cccacgatac ggtggtttta aacaggttgt ttaatctcga agtggtcgtc tgtagcgtag
    11161 aattgatctg cttgtaagtt ttcagcgtct gtttggcgtt tttccccagc ccgctgaggt
    11221 acttatcaaa catcgtttcg ccgcgacctt tatagttgct caccagcgaa tccgctgttt
    11281 ttccgctgcc gacaaagcgc cctttttcat cccttaaacg tccatcactg gacgcagccg
    11341 gaactggtgc aggtgaagat ggtttcctgg gagcctgatt ggcttctccg cctgtggttg
    11401 tctgccaggc gccacctccc tgacctgtat ttgaaggtat tttaagcggc gtcccggcag
    11461 gaccaatcat cagtccgttg cgatactttt caaatatcgc ctcaagccgc ctgaggtgtt
    11521 cttcattaac atccagcgtc agaactggca tctggttacc agccatcaca atactcctgc
    11581 gggatttttg agtttgagca gctcacgaaa ctgagctgct gtttttacat tcagaccaga
    11641 atctgcacag atgtctctga atccggctcc ggctgagtag tcgaggatgt cgctgatgac
    11701 gtgttcgccg tcgcgccaga actggcggct gatttcaatg tcagcaatga agcaatccat
    11761 tccgtaagat tcaatgacga gcattgagtg ctccatattc cattgaccac atccatagtc
    11821 cccttcgcct gctccggtga atttatcgac gagacgcatg taaaaaaaat gagctcaccc
    11881 gctacttcgt ccaaatcaat gatttcacgc tccagcgcca tatcaagagg gattgtgtcg
    11941 tagccttttc cctctactgg gtaaacaagg tttgccaggc gaatgacctc attaaccagt
    12001 gtattacgga cccctttatc accttcccag gcattagaat cacgcgagat ccgctccagc
    12061 atcaaaaaag cgatacgtgg ccccgcaacg acgccaagac cttctgaaaa aatggcagaa
    12121 aaagttttac tcagaatgaa gaaatgctct ttaaacacct ctttgctgat cggcgtggca
    12181 tggatccagc cattaccctt ttctgtccgg acaggaataa tcagattcag atttcgggag
    12241 attttcatac cagatcccac atttcagagt tgatgtaata cgtaccggta atggtgatgg
    12301 ccacacccgg ctcgcccccg gcgaaggtca tatcctgcac gttggtgatc gccgtgttat
    12361 agatatcgaa gtcaccgaat accgtgctgt cgctatacac ttttgcgtcg ccgatcgtgg
    12421 cattcttttc ccattgcgcc ttgaattgtt ttcccagcgc ctggctacgc aacagatgaa
    12481 cacgcgcctg taaaatcatg tatggctgcg gcgactgcac ggctcccgtc atagcgggta
    12541 aaaactccgt gatattgccc tgaaaggaca attcgacgcc ttcttttgcc agaaatgagg
    12601 cggacacatt cagttcggaa tgagaggtga atttaacgct ggcgcgaacc cggttaaggg
    12661 tgccaacggg gatcattgga ttaggcacgg ttcagtccct cacgaaagct gcattgtcac
    12721 attgatgtta aaaatgattt cgacaaatcc gcgcatcggc gtataggagg ccgaaaggcc
    12781 tgcataacgc ccgataccgt aatcattcgg attagtgttg atatactggc ggaaaggcac
    12841 tgcatcgacg acaggctggc cgttgacaag gccgtaagat acgcccgtat tgaacaccgc
    12901 ctgtgcgacc tgctgtagac ggtcgattcc gtcctggttg tagtaaagcg ggttaattgg
    12961 gttattgctg ccgttgatca ccgtgttggc gagctgcata tcgacgttaa tctgcaccca
    13021 gtctacggaa taccagtacg tcatatcgtt accgtcactg gtaacgcctt tcaccagaat
    13081 cgtgttggaa attccaccct cagcacccgt atcgacgtag ttaatattct gctttgtcat
    13141 cgtggccaga atggaatttt tgcccttgtg ggcgtttacc gcctgcagat agcgaaatgc
    13201 catcggcggc accttgttga tttctgaagg tgatgcggaa acatagttcc acattacggc
    13261 tgctgccgcg tttgtcgccg ggtacgtatc atccgccgtt gcaataaccg acttaatacc
    13321 ggcataaggc gaaacgtaat tcgtgtcgtc cggcgttttc gtcagcacga agaaatactg
    13381 catcgcttcg ttggcggtgt ggagttttgc cagactgata aattctgcgt caccatccca
    13441 cggctgcggc accagatacg cataaaaacg caggagcgga tcttccatat aagctttcag
    13501 tgcagcgatt tccttactga ccccgccttt ctgtactccc agctccagca ggtaaatccc
    13561 gactgaattt ccctgggcaa aaaacgtgtt tactgccgtc accagatttt cactgccgac
    13621 aatggaaaac tgccccagtg tcactggcga accggaaagc tgagaatcag caatcgtcca
    13681 ggtcagtgtt ttttcatccg tgacggtagc agtatattcg ccattccacg cgtcgggcga
    13741 acacccggac accacaattt tcacctcaga actgttttcg cgtcggatgt tactcccttc
    13801 cggaagcgtc atcgtaacgg tgacgtttgc cgcagatttt cctgcggcag ccgccgacaa
    13861 cgcagcaatc ggatttttaa ccagatcgtt aatatcctga ttacgggtga gtaatacagg
    13921 ctttcccggc tcatgagtcg tggaaccaaa cgagagaatc gcagccatct gttgcagatt
    13981 cgagggtatg gccccgatgg tctgggacac attcaccgtg acgatattaa atcccattat
    14041 ttaacctcat atttacaaat aactttttca atcaactgcc gggatatttc ccgggcggtg
    14101 ctctggtagt aattcacgtc aaaatcgaca atttttttct tcgccagagc gttgatttca
    14161 acctgtcccg actttgcgtc ctgaaccacc ggaatattgg ttacgccaaa cttttcctcc
    14221 tccagcgccc tgttcaccac cgagtcgaca agatccagcg ccattttgtt gctgaatccg
    14281 taaagcgtca ggcgaaccga atcctggacg agctgaaacc gctcaccgcc gaaaacaaca
    14341 ggagccacct gcaaaggaat actgttgcga acatccaccg cgatatacgg aggacgaagg
    14401 ttctgcggta ccaggtaaga cggatacacg gtcgcggcat ctttcatttg cagccagatc
    14461 gggatgctgt tggagatgat ctgctcgtcg ctgatatcct cctcgcagtc gataatctga
    14521 gagcgcatgg ttggtaaaat cgccataccg cgataatgaa aaatacccga ctgctgataa
    14581 cggctctcca ttcgtgaaaa agcaaactgg acgccgccat actcaccgag atagatcgca
    14641 tcgggatttt ccacattaaa atcatcaacc tgctgaacag gcgtgaaaat aatgttgttc
    14701 acatccttcg agacagactc gtcctgaatc gcaataacct gacgatgcag actgcctttg
    14761 attttcagac gagttggtga ctcaatattc aggcgacaca gttcatcgca actgatgatt
    14821 tccgcattaa cccaatagac aaagccatcc agcggcagaa cctgccggac atagagcctg
    14881 aacgtgattt cctggtctga cgagatggtt tcaactgcgg atttaagaac ggacgagagc
    14941 tgcgaactgt gctgttcggc taattcctca agactcggca ttattatcta tccacgctat
    15001 aaagctgccc ttaaacaggc cgccgtctat gaatgacgga cgtcgctctc cggtatattt
    15061 gtccttaagc ctggagttaa cgcccagtag cgcggcctga gttggcacag ggtttccgtt
    15121 aatcgtcatc ccggccattt cctcggcttc cagaaaacgg tgaaaaatcc cgcgggtatc
    15181 gcccataacc tgcacaggtg gcaacggcac tttatttttg agatggcgaa tgagctggaa
    15241 cgcaaccatc tcccctgcct cctggataat ctcgtcctta tgcatttccc agaagtgcgt
    15301 aaaaatttcg tatcgctcct cgaggtcaca ggctacgtcg aacgtggttt ttcccggttc
    15361 gtcaccgtag tcatacggct ggtcaacaac accaaaacaa agtttcatgg cgtgtacccc
    15421 cataccgtgc ccatctgcat cagcaccgca acaacctgtc gtccatacgg atcctgtaac
    15481 atctgcaaat ccagcaaaga cagattactc agcgcgtcgc tgatggtgat cgaacccgat
    15541 gtcccctggt ctgctgccgc gctgacaagc ccggtagcca gtttcccaag gttgagtttt
    15601 tttctcaggt cggcaaacca cgagccggga gcgaaattca ggagaaatga ggcggcagcg
    15661 ttataaaccg ttcgcacata gatgatgggt aaacgctcca gcccctgatc atgagggatt
    15721 agctccatcg cagactgaaa gcaacattcc agcgtcggat catcgtcagc aatagcgtga
    15781 accggtactt tcatgtcgtc acgaacaaag cgaataaacc cctccagtga cggacgcagg
    15841 gtcattattt tttaacctta atatttcgct ttgtgctggg cggattttcc tgttcggtgt
    15901 taaccgcttc cccggtaatt tccatttcaa taccccccgg ttgaggtttt tcgccgctct
    15961 gaatcaccgc ctgatccact gcgttattca gcgatacggc gctggccgcg agaatttcct
    16021 ctgacatgga ttccagattt tccgttttct gctccgcgca atcctcaatg cgaccgacgc
    16081 tcacaggttt atcgatggaa tagcagatac cggaaaaatt cttgtccacc ttgtcacaac
    16141 gctggaatcc gtaaggctca tgctgccgga tgatgtggtc gataatatcg gactgatttt
    16201 cgatcatatg ctgacgtccg gacggaattg tcacaccgaa cgactgcatt ttttcgggga
    16261 gtttgtagtt gaacgtgtgc ggctgacgtg agcagttagc gatgtagagc ttcataaatt
    16321 tttcccacaa aaaaggggag catttagctc cccgcgttat cagattgaac gtttatgcgt
    16381 atttggcaga caacagggtg atcccctccg ggcggaagtt ccagcccggc gtcgcgcgca
    16441 tggtatacaa cgtggtcagg ccgccatccg gcatagggga cgggatttcc gtcggcgctg
    16501 ccatgtcgca gaacatcacg ttaacggcct gctggttcgg aaccagcgtg gagaagatat
    16561 tggtgttaat ggcgtgacgc gcttccggca cctcaatcgt cgggttcgta acgatgatca
    16621 ggtcattacc accagcacct ttaccgatca gcgtgtcgtc ctggcagaaa atgatgtcat
    16681 cgcctgtcgc cttatcagcg acgtctttaa ccatcgttcc caccgttccg gtaccgccac
    16741 caggacgctg gtaactggtc agctcaacaa ttcctgtcca ctccagcgcc ttcatgaatc
    16801 gctgcggact cagaatgaca gtcgttaatg gctgccccag cagcaacatg cgggttttct
    16861 ggtcagcaat caggccaagc ataaatttag ccatctcgcc ggaatcccag gtggtgtaag
    16921 aatcattgcc tttgctgtcg ttgcccagat tcagcgtcac tgcgttcggt gagttggtga
    16981 tcccctcgtt attagctgca ttcacgccat acagcagcat attacgcaac atttgagcgt
    17041 gtccctggcg gttagccaga cgcaggcctt caatcagaga atagccccag cgatctgctg
    17101 catcagtatc gagatagctg tattgcgaac gggaagaaat tcggtaagtc atcatcccgt
    17161 catagccgcc agagatactg gaagacggca actggcccgg cagagactgg ctgacctgcg
    17221 cctgcgaggt catgcgcaga tatttctgat agaccatcaa atcactggaa ctgattttta
    17281 ccgctggagc accgccagcc aggacttcaa acgcaccgga agccatgctc tgttgcacga
    17341 tcatttccgg cagcaccatt gacggcgaca caatagtagt cgcgggagta aatgcgctca
    17401 ttaattaata tccccttaaa ttaaaaacag gccgcacggc ttaccgattt cccagacaac
    17461 gttaccgccg tcctcttttt tcaccgtcag gtttccgtca actgaaacca tcagcaactt
    17521 aatgtccact ttcgggttag ctccgggcgt tcccgaataa acatcaacca cgtttttcgt
    17581 cagatcccac acaaaaccac tggcagcaac ggtgttattg ccatcagcca acgcaacaac
    17641 ttctgcactg accgggagag gaatgcgggc gcctgagcca acgcggtaat agtgaacaaa
    17701 gccacccgcg agatataacg gcaccggatt atccggcgtg gtaatgccat gaaatgcctg
    17761 attaaagact gtaaatgcgt tacaggcgtc cgtcgtggcc tgcttgataa ccgccccgtt
    17821 aacgctgtct ttcgcgggag cgatgcactc gataacaccg acaccacccc ataccggttc
    17881 agtgattttg ctgtccagtc gtccggagca aagttgcaga cgaattgccg gatcatcctg
    17941 cgcatccccc tgcatcagcc cacgggattc gacgttaaaa aggccaccaa atgccccacg
    18001 gtttttaaac ggatgaaagt taatatcagc cattgttcag gctcccttga gtgttaattt
    18061 ttgccagacg acgccccgga attttgaagg cactcagcca gacgttcgga tcgccctgat
    18121 attcagtaat gcgacgcccg gcttcgtcgt tgcggatgcg tttatgcagt tgcccctgcg
    18181 tactcatcat ttctttttcg atggactgac gggcggcact gaaaattgcg tcttcaagca
    18241 cagccagcgt tgcagaatcc gcaatcgcgc gaatattgac gtccttatgt gccggagagt
    18301 gtttctgcat agcgataagc gcacgcttgc ggtagtccag cgcattttca ccagaaaacg
    18361 gtgctggcgc gtttttaccg caggcactga atgcggagtc ggcttttgcc tgcgcttctg
    18421 ccagggcaga atcattgcgt tctttttctg cctcctcgtc ggccttacgc tgttcttctg
    18481 cttcggaatc agcctttgct ttctcctcag catctttggc tgctgcctcg tcagctttgg
    18541 ctttttcttc cgcctccttt gccgcagctt catcagcttt acgctgttcc tctgcggctt
    18601 catcggcttt ggccttttct tcggcctctt ttttcgcctg agcttcggcg tccgcccgcg
    18661 ctttgtcccg ctgttccagt gagtccatgc gcgtaacgac accatcgatt ttctgattaa
    18721 tgcctttcag ggcatcgctc acaaccccct gtaacagggc ctggagttct tctttttcca
    18781 tctcaatttc acctgtattt gtcacttcaa cccctgcggg gatccggtct ttatcccaca
    18841 cgcccagcga gccgtgggct ttcgtcacca gagcgatgtg atcaacaagg aaaggaacgc
    18901 cctcgattaa aaaattggtg tcaccttcct gtacttccac atttcctgat gtgctgttga
    18961 acaccaccga cggacttgtc gaaacatccc cctcagtgat ttcttcaaca atgctctgga
    19021 ggtaaacgcg gcacaccgcc catacctcgt caccccggat atacggcagc atgacactgc
    19081 cgacgatccg cgatttaaaa tcctcctccg tcagaactgc gtcgtcagga tggtttgcga
    19141 taaccggaag gccattgcat cgccttaaaa actcctcact cagatagagc tttggatcac
    19201 gccatacgtg ctctttcagc ccggcgcgat aggcgagccc ggttccggtt attcgcaaat
    19261 tcaccagcca catgttggag aatttcaccg gggacggtac ggttccgtcc ctgatgcgtt
    19321 ctgccacttc aagctcggtt aaactcacgt ttgcctttct ccgttaaaaa ttcgtcgggt
    19381 agtttctgag gggcgtagat cggcagtgca tcgcaactgc aataaacctc ctccccggca
    19441 gcagtgattt cgtcataaaa accatatacg ggcttaatca gcccctgctc cagcgcccac
    19501 gaatcgcgga ggagataaat tttctcgtcg cgctctttgt ggtcctgtcg gtatttgtag
    19561 cccggacgcc gccagttaga atgccagcgc agagcaatcg ctccactctg aacagccagc
    19621 agatacttaa cgttgcttgc cagcttatgc ccctggtcaa ttgccacccg gcgactgata
    19681 aaatccatat ccttcacgga cttctgaaac ccggccttca cttcccggcg atcaatttcg
    19741 ctcaccccgt caggcggaat ggacgtaacc cacccctgaa aacgctgtat ggttttctcg
    19801 atggcctgtt cgcggttgag ttttatcagg ttggcactgg cgaaaattcg cctgtcgagt
    19861 tccttgcgaa actcaggttt cagtttttca acagtgattt ttttagggcc gtcaggaggc
    19921 tgatcccgta atgccccgcc gtcgatgaca agacggctgt agatagcggt gagatgtttt
    19981 ctggctacgg tatcatcagg ggtttctcgc tgagcggcta cacggagttt ccggcaccat
    20041 tcgagcaatg atttttcgct atcccacccg tgattcacgt agtagttaac ggcatccgtc
    20101 agaacctcat acagcgtcct gatccgtttc ttcttcctca ccgcccggct ggaaattgcc
    20161 atcaggcgtc tcctgcttcg gtggttcata attcgccagc gcgtccacat caatgatgag
    20221 tggagcttcg ccataggttt gtgtggcatt aacaaggctt gccagccatt cagcgagggc
    20281 ggcgcggttt tcaggatcaa cctgtggcga cacggcagag aaaagtgcaa tcgcctgttg
    20341 aatcacttta ctgtcgcttt cccggcgttt gtccggcgac tcctccacca gctcctgcca
    20401 cgtcgcggta aattcacgtc gccactggta aaacgtggtt ttatagtcgt cggttatgat
    20461 gtccgggtaa tcatttttca gcgactgata aaattcctcg ttccaggcga tgtactgcac
    20521 caggcgttcg aaataatcca tcacaggttc aatctgctgg cgtacaccat cgatatactg
    20581 gctgatggct ttcgagtcct ccttaccttc accgaatccg ttactgaatg cttcttcctt
    20641 gataataatg gccggaacgt cactcccggc ggcaatatca gcaatgatgt tgtcgcggga
    20701 tgtattcagc gccccgtcga tgttttgcag gttcagtgaa cttacatctt cgtcttttcc
    20761 aatactcaac acgcctttat ttttggcggc tttcacgttt tcccttttac gccccgtggc
    20821 agcagccatg atcccgtcaa ttttcgaacc atactgcaca actttagcta ccagtacgcc
    20881 cgccttctgg ctgacgagat cattagcctc catcgtgttg atataggatt tcagggaata
    20941 aagaacgcgc tgaaacacac ttcgtccggt gaatccgaac gatgaattct gaaactccag
    21001 ataaatcggt gtgccgttga agattttcag tgtgcgtgac ggatgccagt cttttccgcc
    21061 aatcttcagc tttttattgg cttcctggaa aaacggactg tttgggttct ggtcagtcac
    21121 catcgaaccg gaagcgttca acggatccca cgcgttgata tacacatcct cttctgtcag
    21181 tccgaacgtc ggaagcggtt cacgacatga aacactgtcg gtgcccacgc cgatcgctgc
    21241 agcaccgtag caacgagaca gaaaaaacag atttttaatc ttctcgttaa ccttcatgcg
    21301 ttcccatacc tcctgaaaac gccgcacaac ccgctcgtca gggtctgtct ccacgttgta
    21361 ctggcgcggc ttacacatcg ccatcagtat gggtttttcg acaagtttcc cgcccagcgg
    21421 gtggtactgc cacagcagtt tacacagttc atagccaatg tcggttcccg gctgaatttc
    21481 ttcagcctca agaatgcgca tcaacgaaga actgagattt acagaaatct cgatctcagc
    21541 catcagaaaa aaatccagat ttttacaatt ccgcgtaatt accgtgcgcg atgatcaatc
    21601 cataggtgta acaatcgaaa aggtcatcag cacgtttatg cgcatctttg tctgccaggt
    21661 ggaacccggc gatttgtttt atgaggtggt ttgcggtggt gcgtttgaat gaaacggtct
    21721 tgtcgtaagc ctcccggacg attttgcaca tcccctgata gtggtagctg gatgccatca
    21781 ccgcccgttc gtctttgccc ttgctggtta gtgccgattt aatcggcgtc atatcccagc
    21841 cttcggtttc cgccttctgg ttgaggattg cccccatcgc ggcgtcttcc ataaaaatac
    21901 cctggctgcc cagacgcgga cggcataatt tcgcgaggcg ctcgaggttg tcataaacgc
    21961 cggggatata ctcaggaagc aatgacgctt taatttgcgt cacatcccag tcaataatcg
    22021 tcagttttgg ctcgtccgaa tacgttgact cataacagaa atacaccacg ccagtaccat
    22081 cattttcggt cccgcctttc agcgccgtat ccatcactgc gaaaatcatg tcgcagtacg
    22141 gcggcatctc aattggttga ccgtccacca gcagcttatc gacatcgagt aacgcgtctt
    22201 tggaccagtc tacgaactct gcaagatatt cctgctgcca gacgcgcgga tcggattttt
    22261 tctccgtttc ctccagttct tctttcggaa tatacggatt cgatgaagtt ggcgcatggt
    22321 gcataacaaa tcccagggat tcatcgtggc atatcgcgta gaaaaaattg ctctcgtcga
    22381 taccgtttgg tgtggaaaat acccacgcac agccgcggta atcgacaagc gtcggacgta
    22441 tcgctcgggg ccagatttcc tcgagcattt ccggcgattt agtgaatgcg gcctcatcaa
    22501 tcagcacagc gtgatattta cgcccacgcc cggccagttt gttattgtcc gtgacccaaa
    22561 agtcgaggcg ccccccatta cggagaatga tgcgcttttc attttttgac tgactgagga
    22621 tcagcggttg cagaacggcg ctaatttcat cccagatttc ctggtactgg cggtattgcg
    22681 cggtaaaaat ccccacccta cccgcgataa gttgcccggt ggtaggaatg gcaaatttcc
    22741 gcgtagcgaa actggtagcg atgttcacca gcatcaccgt tttaccccag cgacgaccac
    22801 agcataccgc gtggaagcgt tcctctattg ccgccgtcca tgcagctatt tgcccctcat
    22861 gaggttttgg gaggtagatt tcaatcgaca ttatccactc ccggcatcgg cagagagttg
    22921 tggataatta tttcgttatt ctcaccaccc acgccttttt tgaggttttc aatctcagtg
    22981 cgcagctttt cgttgcgaag cctcagtcct tcaagctcca gctcattgcg actgtcagtt
    23041 gcatcaccag cagaacttcc tttcgtcgcc attatcagct tgataagttc gcgccgggcg
    23101 gcagccttat cctccagcag gatctcaaca ccaaatttcc cgagctttgc ccctgcatat
    23161 aattgtcgcg catccccatc aagcagagtg gtatcagcca tataaagctg tcccgttccc
    23221 tcaccgcagc acttcggaca atccggattg ggtatggcgt tatcaacaaa gccaaggcct
    23281 ccatattccg gctcgggttt gccatctctg gaggcctgtg ccgctgcctt atcgaactct
    23341 gcaatatcac gccactggta gaggtgattc tcgccccagc aataacggca gttaacacgg
    23401 cgaaattgtg ccaactgatt ggggtcggcc tggacaatgg ccatcaactg actcaccagt
    23461 aaatccaggt ctgcggtata gcgtttctgg tactgattgc ggaaccatga gatagcccga
    23521 taaaccttaa catttcttaa cagacgtgag gctgcggcac ttgctacgtt tcccgttccc
    23581 tcgtaacctg ctaggcggta tgcctcgatg aggtttttac cctgcgcaac cagcatggcg
    23641 aattttgcct gctggtcaga aatgccgaat tcatcgggac agaatgaaat ttcctccgcg
    23701 tcgccctcat tcaggcgcgc atcggatact gacttttttt tctgagattt tccgttccgc
    23761 ttttgcgcag tctgcgcaaa ttttttctgc gcactttttt gcgcagtttt gcgcacttct
    23821 gtctgcgcat ttttcgaagg ttttttgatg taacgacggg ctgttgcgta attcagtccc
    23881 cttgcttcac accatgccac cggagatata ccggagcggg tgtattcagc aatatactcc
    23941 tgctgcaacg ccccccagtc cggtctgctc atcagttagt cctgattttt atccaccctg
    24001 agtagttcgc gcagggcaaa ggcatcccct tttctggcaa gcttaaacaa tgccgcccgt
    24061 atctcggctt cacctttcgc tctgccctta cggatggacg cgtaaaaatt tgtcattgct
    24121 tcccgatttt ctttcaaccg gttcagatca acatccagaa cgtcagcgat ttgttgtgca
    24181 gtcatccggc acgctgccag agactcgact ttcgaatacg gaatcatttg tcacccccat
    24241 tgatatgcag ggtgtcttct tcctgtattt ttcgtgaagg atttttactg cagcgttgtt
    24301 ccaggtgact tgatggtgaa tgcgtttatg gctggcgccc atcagtgaga tttttacgca
    24361 cgacggcgca tacatgacgg agtaaaaact tttaacgtag gttccggaat ccagatacag
    24421 ctcggtcatt ccgccgctgt ttttctgcgt ctgtttctgc cctaactgga cagcaccgat
    24481 cgtcataaac aattcaccac ggcgaccgaa attcgtgtac gtattcacat cctcgttaat
    24541 gcgccccatg aatgagaacg gtcgatcaac cgaacagata aagctgttca ttgccttgcg
    24601 tttcacccac gaagcatggc cgccattgtc accaagaaaa tccccgccct gcgacatggc
    24661 gatggaaagc gcaggtattg attcgtagta cgccagcatt tcagaaagga tcgcatccag
    24721 ttttcttatc gggaaatagg cctggtcata gttgcgatcc acccgaaact ggaactcgtg
    24781 ataatcatca tcgagctgaa tgaagtattt acacccgacc agttttgcca ggtcgaaaca
    24841 ggcattacgg gcgtaaaaaa ttgagcggcg atcaccgaaa ttatcggctt cgtcaaaacg
    24901 actggctata tcggctttgg aaaacaccag cacctgttca ccaaattcag ctatgtactg
    24961 atgccgtgtc ttatcttcat catcaacaac gataaaaact ttcccggtat agccagcacg
    25021 acgcaacgtc cggtaagtca gaactttgtc cgggcgcccg tgagtcagaa taaaggcgca
    25081 aaaatcatca cgcatattcc tcctcctccc cgccatgcat gatctccacc atgcgctgcg
    25141 tcatccggac aaatccattt tcaatagcct gctgataatc aatgatcacc agcgccgact
    25201 cctcaaaaag gttctgaatt tcagcggggg cgtgaacgta atagtccgca attctgctga
    25261 aattaaacac cgtgtgacgt tctgccgcgc acaggaggaa tttttcgata tcaggctcaa
    25321 gggacgccga acgtatccgg ctgaccagct cctgagtttt cgtatcgtcg tacagttcac
    25381 tgatatccgg tttatcgcct gacggctcat aaacaggcgt atcaattttc gtcgtgtacg
    25441 gctcctcctc atttcctgta cctggcaaaa catccgtcaa caattcatca atttctgttg
    25501 ggctgaatcc tgtcagggag acatcaaaat cagcattgat taggtccgac agttccaccc
    25561 gtaacagttc ttcatcccag ccagcgttga gcggcaggcg attatctgcc aggcggtacg
    25621 cctttttctg atcatccgtc aggccagaca gaacaatgac cggaacagaa tccattttga
    25681 gcacctcagc cgccatcacg cgaccgtgac ccgcaataat ttcgcccttt tcgtcaatca
    25741 gcaccggatt ggtccagccg aattgcttaa tgctttcttc cagttgtgcc acctgctcag
    25801 ggctgtgtgt cctggcgttg tgcgcatacg gagacagttt ttgtaacggg cgatagacga
    25861 tcttcaattt ctcgctcata cagcctcgct tgatgaataa aaaagctcac taacagccag
    25921 tgcgctgggt gcgcggcggg tgctgttggt gagcctggca ttatcgcagc ccctcactga
    25981 agggctgctg taatgcctta acccagtttc gctcgcacca gagcatcttt tgcttcaagc
    26041 aatttacgta acccagccga cttttccgga ctgtctggca aagattcatc cattactctt
    26101 gccagatcac caattggctt actcacttcc tttaacgaag tcggaagatg ctcataattg
    26161 aaatatttca taattggcga ttccatcatt tctccttagt aaaacaatta tcaggcgatc
    26221 agttctgcat acactgccgg acaccgtcga caatttcaca gacctgagaa gctgtatcga
    26281 aaagctggcg cgccttatcc aggctgacgc atcccaccaa taaaaaaggc accagtatcg
    26341 ctaccagtgc ccatttcgct gccgttcgcg gcattctgtg tgtccggtgt tttcgcgtca
    26401 tatcaccacc aacgcacagc ccaaatcaga acagcgaccg ccacaaggcg aattgcaaag
    26461 gccgcagccc ttgtcaaatc aaggctcgcg ggagtttcca cttcaatacc tttcataatg
    26521 gacaacctca aaaagaatct tttatacttt cccacgagga ttttctccgt actcactact
    26581 cacaatttcc tctctgacgt gaaaactaaa aaccccggac tgttccagca gccggggttt
    26641 tgttttttat ttactgccct gggtgcggca tctggctatt tcattcctgg ctttactgtc
    26701 accgcgacag atacaacgca ccgtgtctcc ggtcagcgtg gtaatgtaag cctcatcctc
    26761 tttttctacc ggaatacatg gaatatcccc gcccaggcaa tactcaattt ttgctgcaag
    26821 atgagtgtca cgcggataga actccaaacg atacatatcc cccagaacat gcacttcttc
    26881 aacctgacgc ccatcttccg ttaccgtgat ttttttcagt gcgtacatac gtacctccgt
    26941 tctttcgttt tttgagcaat aaaaaagccg ctcatgacgg ccctgtatgt tttgcaagct
    27001 atcgcttcaa ttgaaaatgt ggcccgtctt tcagtgtttt ccagtccccg ccccattcga
    27061 tggcagttcc cagctctgcg gcagcctgct taaatgcctg cgcgattttc tcgtacagag
    27121 gccagtccca tgacacctgg ctgccaatgt aggccacaac atccaccgca tcaccggtca
    27181 ggtggcggct gttcatggtc tggctcttgc cttctgcaac cagctgtttc tggcgttctt
    27241 tcgtgcgcag accttctgta acaccgaaat caacctccgt cagctccagc gcacggcgaa
    27301 cgacagcaac cagctgtggt ttgacgccct ccagattttt ttcactgcga cgactgaatc
    27361 tgaatttacc cgacatgctc acctccggaa tgaaaggatt tttgaaacgt tcccgcgtgc
    27421 gcgtattacc agcacgcaga acagcaaatt aagccccacc gccagccagt tcgccgctaa
    27481 cgggcgacca cacagatagc tgaggggcgc aaaggcatac agcagcatca gcagccaggc
    27541 cagccatgac atcagcggtt tgtgtctgga gtcacgacga cgataaaaaa agagcgtcag
    27601 cacgataacc gtgcataacg ccacattcag caatccggga aggttactta acattgccgc
    27661 ctcctccacc ccgcaggcgg gagaacaggc cggacaccag tgatgcgata tcctgctggt
    27721 ggatgaacga cagaatcttc accgacacta ccgccaccag caccgcacac agtgcatccg
    27781 cagatgtacc gtcataacct gtttttgatg caatccaggc tgacagtaca cgcgccccca
    27841 gtacaccaac aatgaacgac accagaaaat gtgccgccac acgccaggct gaaatcttct
    27901 gcggcatcgt tgccacaaac aacgccccgg cgaacgcacc aaacacaatc ccgaaatccg
    27961 ttccggtaaa cagcccgaat accgtcgccc cgccgagcgc cgcagccgtg ccggaaccgg
    28021 ataagggttc agacatactt tttctcctgt aaataaaaaa gggccaccag cggcccgtaa
    28081 aaaacaacac cccgtcaaag gcacccgcag atgccttttg tgtggtgtta ttcagatttg
    28141 cgcagtaaag gccggagtac ggccagcgcc atcgccacca gcacaccatc tgccagcacc
    28201 gacatcagcc gtccggtgaa atccaccacc actaccagaa acagcaggat ggcagccagc
    28261 acaaggcgcg cacttttcac aggtactgct ccagcggtaa ctgcagcgcc tgagcaattt
    28321 tcttgagctg tgcttcttca tccggaccaa tgccatcctg gtcagcgata tccagacaca
    28381 ggcacagcac attaactgca tcatcagtac cggcaacatc agccagctga cgaagggctt
    28441 cggcattggc agaacgcggc gacgcttcat aacgggcgcg gatatttgca ctcatttgtg
    28501 caatctcacc ggagaacggc gcaaaggcag gaagtgctgc aatggttttc tccagtaccg
    28561 cgacttcttt cgcgtcacat gtgtcgtcag catatgcaat ggaatacgcg ccccagacgg
    28621 tcgcctccac tgcgtcgcgg ttctccatct tcttcacttc ggtaatggcc ttgcgggttt
    28681 tctttttgaa aataccaaac atcgtgactt ttccttttag tgggtgagcc tgcgcccggg
    28741 ggtgaccagc ccacagagaa agtcacactg accatcccgt aagctcaccc ctgaaaggct
    28801 ctgtggtttt tgatgtgcgc cgggcgtggc gcgaagaaat gaaataaagc ttatctgaaa
    28861 ttaaggttaa tacggggatt taaaccattt ttaaagctta gtaatatcaa ctcgtctccc
    28921 ggaggagaca gatgcttatg attcttcacg gactttgtcc cgcggcttta atccgacggc
    28981 cgcgcctttt ttcaccctcc cgcaaattgc taaccaagga ccttgtccca cgagtattcc
    29041 agaatgctcg tgtctttttg tccggagaag aaataaaaaa aaccgcccgg tggaggcggt
    29101 aaaggatgta tttccaggtt ttgcttagta tatgattaat ctcaatgtca tttttagcga
    29161 tgtttacaac atcgggatgg cacatcaccg accctgtcag gaaatacaaa atctccaccg
    29221 acaatgcgcc attccgatgt tataaaaaaa atcagcactg ataccgtaat aaataacatt
    29281 cattacagga cgggatgtag taaggagtca gggtaaaaca aaaacatccc acacatataa
    29341 tgtgtcagtg cctaacacaa cctagcatct attgtcctct gctggagcgg ggtggcggga
    29401 atcgaacccg catcatcagc ttggaaggct gaggtaatag ccattatacg atacccgcat
    29461 atggtgccga ctaccggaat cgaactggtg acctggtgat tacaagtcag ttgctctacc
    29521 tactgagcta agtcggcatt ggctcttcag gggagcgata tcaccgatca aagaagagtt
    29581 ccccctcaga gccgttttcg atgatacgat ttaatactcc aatcgcaaca acaccttgcg
    29641 tcaagttatg taaatttatt tatatgtttt tattttatgt gaataattca ctttcactta
    29701 aaatataaat gacaatatat aaacaaattt attttgaagg cgattattaa atgtcgtttc
    29761 tgatatcacg ccccagaaac aacaaaaccc gctcaatggc gggttctggt aaagttcatg
    29821 cgcttgattc gcctcgcgat acagctttgc gaagcgtacc gg
//
LOCUS       DAHBHC010000064        29095 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHBHC010000064 DAHBHC010000000
VERSION     DAHBHC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.064"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(267..2003)
                     /locus_tag="M2A35_003366"
     CDS             complement(267..2003)
                     /locus_tag="M2A35_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCB7199620.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(2098..3012)
                     /gene="ldcA"
                     /locus_tag="M2A35_003367"
     CDS             complement(2098..3012)
                     /gene="ldcA"
                     /locus_tag="M2A35_003367"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCB7199621.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            3112..3723
                     /gene="emtA"
                     /locus_tag="M2A35_003368"
     CDS             3112..3723
                     /gene="emtA"
                     /locus_tag="M2A35_003368"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCB7199622.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(3725..4459)
                     /locus_tag="M2A35_003369"
     CDS             complement(3725..4459)
                     /locus_tag="M2A35_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCB7199623.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            4660..4914
                     /locus_tag="M2A35_003370"
     CDS             4660..4914
                     /locus_tag="M2A35_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCB7199624.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            5092..5532
                     /locus_tag="M2A35_003371"
     CDS             5092..5532
                     /locus_tag="M2A35_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199625.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(5611..7308)
                     /gene="treA"
                     /locus_tag="M2A35_003372"
     CDS             complement(5611..7308)
                     /gene="treA"
                     /locus_tag="M2A35_003372"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCB7199626.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(7628..9046)
                     /gene="dhaM"
                     /locus_tag="M2A35_003373"
     CDS             complement(7628..9046)
                     /gene="dhaM"
                     /locus_tag="M2A35_003373"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCB7199627.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDELLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(9057..9689)
                     /gene="dhaL"
                     /locus_tag="M2A35_003374"
     CDS             complement(9057..9689)
                     /gene="dhaL"
                     /locus_tag="M2A35_003374"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCB7199628.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEPNLSVPVALEAASR
                     IAESAAQSTITMQVRKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(9700..10770)
                     /gene="dhaK"
                     /locus_tag="M2A35_003375"
     CDS             complement(9700..10770)
                     /gene="dhaK"
                     /locus_tag="M2A35_003375"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCB7199629.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            10998..12917
                     /gene="dhaR"
                     /locus_tag="M2A35_003376"
     CDS             10998..12917
                     /gene="dhaR"
                     /locus_tag="M2A35_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCB7199630.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(13017..15884)
                     /locus_tag="M2A35_003377"
     CDS             complement(13017..15884)
                     /locus_tag="M2A35_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7199631.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAEQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNSLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(16725..17816)
                     /gene="ychF"
                     /locus_tag="M2A35_003378"
     CDS             complement(16725..17816)
                     /gene="ychF"
                     /locus_tag="M2A35_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCB7199632.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(17933..18517)
                     /gene="pth"
                     /locus_tag="M2A35_003379"
     CDS             complement(17933..18517)
                     /gene="pth"
                     /locus_tag="M2A35_003379"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCB7199633.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            18795..19073
                     /gene="ychH"
                     /locus_tag="M2A35_003380"
     CDS             18795..19073
                     /gene="ychH"
                     /locus_tag="M2A35_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCB7199634.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(19128..20807)
                     /gene="dauA"
                     /locus_tag="M2A35_003381"
     CDS             complement(19128..20807)
                     /gene="dauA"
                     /locus_tag="M2A35_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCB7199635.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIIIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(20932..21879)
                     /gene="prs"
                     /locus_tag="M2A35_003382"
     CDS             complement(20932..21879)
                     /gene="prs"
                     /locus_tag="M2A35_003382"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCB7199636.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(22030..22881)
                     /gene="ispE"
                     /locus_tag="M2A35_003383"
     CDS             complement(22030..22881)
                     /gene="ispE"
                     /locus_tag="M2A35_003383"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCB7199637.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(22881..23504)
                     /gene="lolB"
                     /locus_tag="M2A35_003384"
     CDS             complement(22881..23504)
                     /gene="lolB"
                     /locus_tag="M2A35_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCB7199638.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            23718..24974
                     /gene="hemA"
                     /locus_tag="M2A35_003385"
     CDS             23718..24974
                     /gene="hemA"
                     /locus_tag="M2A35_003385"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCB7199639.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            25016..26098
                     /gene="prfA"
                     /locus_tag="M2A35_003386"
     CDS             25016..26098
                     /gene="prfA"
                     /locus_tag="M2A35_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCB7199640.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            26098..26931
                     /gene="prmC"
                     /locus_tag="M2A35_003387"
     CDS             26098..26931
                     /gene="prmC"
                     /locus_tag="M2A35_003387"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCB7199641.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     THLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            26928..27320
                     /locus_tag="M2A35_003388"
     CDS             26928..27320
                     /locus_tag="M2A35_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCB7199642.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            27324..28133
                     /locus_tag="M2A35_003389"
     CDS             27324..28133
                     /locus_tag="M2A35_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCB7199643.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            28169..29023
                     /gene="kdsA"
                     /locus_tag="M2A35_003390"
     CDS             28169..29023
                     /gene="kdsA"
                     /locus_tag="M2A35_003390"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCB7199644.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         7227 a         7272 c         7385 g         7211 t
ORIGIN      
        1 aagaatggac actatttccc cggaacctga actcaccgca caggcgttct acataaaacg
       61 cttacgcttc attgttgact cgaactcgac ttcagataaa tcacgcttta cccttgatgg
      121 agcctgtaca tagatttgtg taattgcctg attttgatat gttcaattca acatcaaatg
      181 aaggttaaat tatggacgac aaacaattgc aggctcaggc tgcgttcagc aaagcatcgc
      241 aaccggcgat agatgcttca ttaaatttaa gattcagctt cctcttcagc cacccgtacg
      301 ccaatcttca acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac
      361 tctacctggt caccgacaac cggtgccgcg cctaacaact gctggacaat ttcacccagc
      421 gtttgctgct tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca
      481 gcatatttgg cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc
      541 ggcggcgact ggctgaacag tttaccgagc gccggaagat cgcgttcccg accaattaca
      601 cacaacacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca
      661 cgaaacagtg ccgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcgccc
      721 acgcaccatt tatcggcact caattgataa acaaactgct cccacggatt ttccggatga
      781 atatccaggc caacgcgtga caccggacgt cccactggcg gaaccaccac tttggctttt
      841 ttagccgccc acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag
      901 gcgacattaa agaacagacg tgcattctcc agccccgcca tcatcgggaa cacggccagg
      961 atgattggca ccgcgccgcg taatcctacc cagctgataa acacgcgctc acgcagattg
     1021 aaaccgcgga aggggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc
     1081 catgccgaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaat
     1141 agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc
     1201 aggatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca cagataaaca
     1261 gccagaatac cgctgccttc cagcgccgtg gttaaagcaa aaattaaaat accgccgctt
     1321 aatgccagca atggatataa tccggcaggc agggcgatgc gattaatcat ttgcagcagt
     1381 aaataaccac cgccaagtcc gatgacaatc ccgaggccaa attgctgcag aatatcgacc
     1441 acgaacatcc aactgacact gctttcatgt tgctggatca tcgcaatcag ggtaatcgtc
     1501 agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt
     1561 tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt agagccgacg
     1621 atagcgccga ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg
     1681 ccggttaaac cagaggtgat aagcacgccc agcgtcgcca gcgacagtgc cggtcctaac
     1741 gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagt
     1801 gccaggttac ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca
     1861 tcgactcctg ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg
     1921 gaagaaaatg aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg
     1981 ctaattattg ttgtggcatc caacggtcgg ttactcctga ttacgctgtc tcttatataa
     2041 accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta
     2101 cattttaaga acaggatgac cgctaatcgt caactgagtg ccttcccggg tgttattcag
     2161 aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc
     2221 ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta
     2281 acctgcgtca taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg
     2341 acgtggcaaa attcccgcat gatagagctg caacagcata cgttcgacgc ggaaagggtg
     2401 ctcgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc
     2461 aatcagtgaa atcagcatcg caagattgcc tccccacaac gtgccttcgg tctgacatgt
     2521 cgggccttcc ccttgccatt caatggtgaa ggttttattg cgtaacgcca gccagaaatg
     2581 gtgctcggta aaggcgttca gttcatccgc accaaagttt gctaccagca tggggccgct
     2641 aaaggtgatg acattgcctt gcgccagaag accgcactga atggcggtaa aatcgctatg
     2701 tccgcaaatg agtaacgggt catgttgttg gcgagccacc agtgcctgcc agtcaatatc
     2761 tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt
     2821 agtcagtctg gcaagggaat tgagatcttc caggcgctcc gtttccgtac ccgcaaaacg
     2881 ctcacaacga cgggcaataa cctcgacgtt attgacctga tgcccctcgt cggtaaggcg
     2941 atggatacca cgcagcgcgg cgtgctgttt aatgcagtaa cccgatgggg caattaagtg
     3001 aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta
     3061 ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg
     3121 agatggtttg cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg
     3181 aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc
     3241 cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatt
     3301 gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta
     3361 aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg
     3421 accagcgagc tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt
     3481 ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg
     3541 gtgtcatacg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag
     3601 gcgatcagca aaatcaacga tttggatgct gacgagttcc tcgatcacgt agcgcgaaat
     3661 catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg
     3721 taaatcagtc gcgcactttg tccgcttttt ctcgggcttc tcgctcgaga gagaaaataa
     3781 tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg
     3841 gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctgatg gagattaact
     3901 gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatctga gcgaagcgca
     3961 tgccttcttg taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg
     4021 acaaatcata caggcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat
     4081 aaggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg
     4141 tgggaggcgg tacggtgata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt
     4201 caacagtaaa ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct
     4261 ttagcacggc gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg
     4321 ttattgccag taatttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg
     4381 cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact
     4441 gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt tggtttatcg
     4501 gtcacagtta acaaaactta atacaaatgc gtgaatattt tttacatgtt gttcttaaat
     4561 cagccgacat acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgatgtaag
     4621 catttcttgc gtccgattca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt
     4681 atttttggcc tgatagccgg cattatcgcc aagctaatca tgccggggcg tgatggtggt
     4741 ggatttttcc tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg
     4801 accatgtttg gcattggcgg ctccatcagt ggttttaatc tgcacagctt cctggtggcg
     4861 gtggtgggag ctattctcgt tctgggcata ttccgcctcc tgcgaagaga ataagatttt
     4921 cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga
     4981 cacctgcttt acgggtgaaa aaaatcaata tagcacttcg atatccgata aagtcggttc
     5041 ccggtaacaa caacagaatc cttttttaat tattgtttcg ttgtttttgg tgtgattcag
     5101 aaaataaata ttttagacag tagccagtca ggcgttaaaa atggggcgga tagtgaggcg
     5161 gttttggctg cgcttattga acatttagaa ttgatcaatc cttctggcag attaagccaa
     5221 aatacccgtt cggcaatgct tcagttacgc gaagaggaat ggtctgaatt ctttttttgg
     5281 ttgctaaatt ctctggagtg tctggattat gtgattatta atttaactcc agaaagcaaa
     5341 aaaacgttga tgagtgaaca ccgtaacaat attcaggtag caattgatgc tttatatagc
     5401 caacgtcggc gtaaatcgcc gggtgatgaa agtgaaacac ttacccgtag aaatgacgct
     5461 atcttcggca accacgtgtg gcaaacattt gcgcaatact tcccgccagg acttgaaaaa
     5521 ccgtctgtgt agaaaaaacc atagctatgg tgttatctgc cgacaacgcc atccggagaa
     5581 gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg ggttgtgcct ctttggttga
     5641 gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc ggaacattgt cacacggttg
     5701 ctctttcggg cagatcaaat ccagcatttt cagcgtcacg ccattggtcc agccaaagcc
     5761 atcctgtaat ggatattcgc caccgccgcc ccccgttccg gtggtgctga catcatattt
     5821 ttccaccagc tttttctccc ggtcataggt gtgctgaaca ttggtcagga agtgccagct
     5881 aatgtccatc gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg
     5941 taacggtgcc cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt
     6001 caggccgccg ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc
     6061 tttcgctgcc gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac
     6121 tttatgactt ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acatgtattt
     6181 ttcgatccct ttttgacggg catttgccag cgtttcgtac tggtttgcca tcgcgttatc
     6241 tccggcagct ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag
     6301 atcgaccggt acgatgctgg tggtgcgtaa ggtatttagt tgatgcgggt tgtccatcca
     6361 gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt
     6421 ggcaggtcga ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg
     6481 tggcgtatcg cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gttttacaac
     6541 gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc
     6601 tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa
     6661 ctctaccatc agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc
     6721 gttgggaata tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac
     6781 tttatcccag tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata
     6841 tacctcgcga aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc
     6901 ccatttttcg gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc
     6961 gcgcagtgac tgcccctctg gcggaacata tttctcgcct tctttcggca gggtgaaatt
     7021 gacgttaacg aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc
     7081 aaggatcatc agcggatcgc tgttcggcac ggcatcggca aaggtttttt ggtccggaaa
     7141 aagtttggcg ttttgcacat cattaaacag cggccctaat aaaatatcag gcggctgtgg
     7201 tgttaccggt gtttcttctg cctgcaccga tagcgcagcg aaacacaaaa agatacaggc
     7261 tggaattaac gccatttttt gcgggcgaga aggtgcgggg gatttcatca atcattctcc
     7321 tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa accttagttc aggatcgcac
     7381 tagctgcatg atcgaaatct cattctgcga actgtcaggc attttagaaa agggaattaa
     7441 agggataaat aacggcgaaa tagagtgatc aaataacgtc tgtttattag ccagccagcg
     7501 gcgttgggtt cgtgattagc gaagaaaaat catcataaat tggtgaaaaa tataacaggt
     7561 agttaaaaac cattagtgct gagtaaattg ccggatgaca tcagaacgat gccatccgaa
     7621 cagtggctta accctgacgg ttgaaacgtt gccttttaac gtccagcgtt agcgtttctt
     7681 ctggttgtat cgcatacagt ttctcaccct gctggcaaat ccagccaatc cccagttcac
     7741 gggcgattag ggcgctgtgg gataccggac ttccggcgct aaggcagata ccttttacaa
     7801 ccgccggatc cagttgcagt actgtggaag gataaatgtt ctccgccagt agaatagttg
     7861 gcgagttaaa ctgcgggagt tcttctttcg tttgggtcag gtggaccagg gtgcgatgca
     7921 gaagctcgtc cacatcaata tagcgagctt gtagatattc atcatccagt tgctggtatt
     7981 gctggctaag ttctttaaga acttgctgcc aggcatattc tgccgtgcaa tgttcatgct
     8041 gaaggagttc gcttgccgcc gccagcagtt ccggatcatc taacagtgta tggtgaccag
     8101 aaaagattgc ggcaatatcg tcaagcccgc tggcttctgc ttttgctgtt aacgtcatca
     8161 gatctaataa cgtgaagtca atagcctggc gtaatcgatc ttgttcttct tccacggtca
     8221 gggttgattt tgcctgtacc gtacataaaa ctggttgata ataaaaggct ttacccgaaa
     8281 caggcggaac gggacgcaga gtaggtggag cgacttcctc cgtttcacca aagttatctt
     8341 cagccaactg acggaaagcg atcagtgcct cttcagcttc tggccctttc gcaatcaggc
     8401 gcagcgtatc gttatagcga acttgtagta acgcaatctg gttaatactc tctggtgtga
     8461 cgcatttgcc gtttttttcc agcaacatat cggcattaaa tgtcgataag gtataaacca
     8521 gccgggaggc cggacgtaca tgcaggccgt tacggttttt tatgaccacc gccagagaac
     8581 gggcttcttc atcgtacgca ggacatgtgt cagagatttt agtgtcggag gacggtaaac
     8641 ccagttgttc acgtttggct tccagcgcat gcatagcgtc aaagataact ttgtcgatat
     8701 ccgctcccga ggccgcgctg accgttgctg ccagtgtacc ttcgaccaac ggcgcagcac
     8761 acaaacgtac ttttgcggcg atcttgggag ccagcaattc cagcgcagtt tcagcactca
     8821 ataatgcgct acccatatcc atcatgacca gcacatggtc ggcatcagca acagattcga
     8881 tggcctccat cactttgacg gcatcggtac caataggatt ttgtggatcg tcaattcccg
     8941 cggcaatggc gattttacaa ctatcactca ttaacatctg acgggctaat tcaccgacac
     9001 cttctcccag tcggctgcta tgtgaaacta tgaccaggtt taccatcacc aattccttac
     9061 tcttttgcgg ctaacgccaa catttgcatc ataaacatca ccgaggtcgc gccgggatcc
     9121 tggtgaccaa tactgcgttc accgagataa ctggcgcggc ctttgcggac ttgcatcgta
     9181 atcgtacttt gtgcagcgga ttcggcgatt ctactggcag cttcgagcgc caccggaaca
     9241 gagagatttg gctcgctgga ctgacgtaac gattccacca ccggcaccca cacatcacac
     9301 atggttttat cgccaggttc ggctttcccg cgactgatta cgccgtccac gccatcgcgg
     9361 aacatctgat aaagctcttc cagtgtcagg ctttgccgtg cctgggtcgc ctgtgcggcg
     9421 cggataaaga aggtaccgaa cagcggacca ctggcaccac cgacgctgga aagcagcgtc
     9481 ataccggtat tcttgagaat gaaaccgata tctttatctg cgatagcagg gagtttttcc
     9541 accactttgc taaagcctcg gttcatattt agcccgtggt cagcgtcgcc aatttcgcga
     9601 tccagtccgg taagatactc gctctcggtg ctgaaaatat cgccacaacg agtgagccag
     9661 ttaacaattt gagttctgct cagtgacatt gctttctcct tatttacccc agttaagggc
     9721 cggggtgtgg accggggcgt cccagagtgc cagcgtttcg tcatcaactt tcagtaaggt
     9781 gattgagaaa ccggtcatat ccagtgaggt gcagtacgcg ccaattaaat tacgttcgat
     9841 agtcaatccc gcttgctggc aacgtgtggt caggcggtta tagacgccgt acagctcaga
     9901 aagcggagtt gcgccaagat tgttaaccag cgcaatcacc cgatcgccag actggagcgg
     9961 ttgtttggtt tgttgttctt cctgccaact gccttgttga taatcccaga aacgcaaagt
    10021 gcgatggtat gagccatttt ccagcagggt gtcgaacatt tcatcgacgg tttgatcaag
    10081 ggaagagaag gagcggcggt caatacccgg ctcaccatga atgccgacgc caaactccat
    10141 ctcattatcc gccagggtaa aagaaggttt gcccgcggca ggaacggtac aggcaccgag
    10201 agcgatacct attgagtggc cttgattatt caacttacgc cccagttccg cacaggcgtc
    10261 cagtgagtcg ccacgctccg ccgctgcgcc tacgagtttt tcaattaata cggtgttggc
    10321 aacgccgcgt cgcccggcag tataaagact gtcttttacc gcaacgtcgt catcaatgac
    10381 cacagtggtc acttttacgc cgctatcgtg cagtaactcg gtcgctgttt caaagttaag
    10441 aatatcgccg gtgtaatttt tgataatcat cagtacacct tcgccgccat caatttgcat
    10501 ggcgcattca aagattttat cgggcgtcgg tgaggtgaaa atttcgcccg gacaggcccc
    10561 cgaaagcatc ccctgaccga tataaccaca gtgcatcggc tcgtgtccgc tgccgccacc
    10621 cgacagcagg gcgacttttc ctgcaacggg ggcatcagct cgggtgacat acaccggatc
    10681 ctgatgcagt gtcagagatg gatgcgcttt cgccagtcct gccagttgtt cgtccagtac
    10741 gtcttgcaca tcattgatca attttttcat tattttgctc cagcaattac ggtagggcat
    10801 ggatgatgtt caacgacacg gcgaccactg actgccgatg aatccattgt gcatcaggca
    10861 agggaaaaga aaattagccc aaattatgtt tcatagtgaa acatatgctt taatgaatgt
    10921 tccatattga aacttttacg tgtattaata cttaaaattg cgagccggaa caccttttgt
    10981 cataagggat gcgggatatg agtggcgctt ttaacaacga tggtcggggc atatctccct
    11041 taattgcaac ctcctgggag cgatgcaata agctgatgaa acgggagaca tggaacgtac
    11101 cacatcaggc ccagggcgtg acatttgctt ctatttatcg gcgtaagaaa gcgatgctga
    11161 cgctcggtca ggctgcgctg gaagatgcct gggaatatat ggcaccgcga gagtgtgcgc
    11221 tgcttatcct cgatgaaacc gcctgcattc tcagccgtaa tggcgatccg caaaccttgc
    11281 agcagctaag tgcactggga ttcaatgacg gcacgtattg cgccgaggga attattggta
    11341 cttgtgcgct atcgttagcg gctatctctg gtcaggccgt gaaaacgatg gccgatcaac
    11401 atttcaaaca ggcactctgg aactgggcct tttgtgcaac gccgttgttt gacagcaagg
    11461 gccgattgac gggaacaata gcgctggcgt gtccggttga gcaaactacc gcagctgatt
    11521 tgccgttgac gttggcaatc gcccgcgagg tcggtaattt actgctgacg gacagtttgc
    11581 tcgctgaaac taaccgtcat ttaaatcaac ttaatgccct gttagaaagt atggatgatg
    11641 gcgtgattag ctgggacgag cagggtaatt tgcaatttat taatgcccag gcggcgcggg
    11701 tcttgcgcct tgacgcgacg gcaagtcagg gacgggcaat cactgaactc ttaacgttac
    11761 ccgccgtatt gcaacaagca ataaaacagg cacatccgct caaacacgta gaagcaacct
    11821 ttgaaagcca gcaccagttt attgatgcgg tgataaccct taaaccgata atagaaacgc
    11881 agggaaccag ctttattttg ttgctccatc ctgtggaaca gatgcggcag ttgatgacca
    11941 gtcaattagg aaaagtcagc catacctttg ctcatatgcc acaggacgat ccgcaaaccc
    12001 gccgcttgat tcattttggt cgccaggcgg cgcgcagtag ctttcctgtc ctgctttgtg
    12061 gagaagaggg cgtgggcaag gcactgctaa gtcaggcaat tcataatgaa agcgagcgtg
    12121 ctgcaggtcc ttatatcgcc gtcaattgtg agttatatgg tgatgctgcg ctggcggaag
    12181 aatttattgg tggcgatcgc acggacaatg aaaatggccg tctgagtcgg ctggaactgg
    12241 cacacggcgg cacgctgttt cttgaaaaga ttgaatatct ggcggtggag ttacagtctg
    12301 ctttgcttca ggttatcaag cagggggtta tcacgcgact ggatgcgcgg cgtttaatac
    12361 caattgatgt caaagtgatt gcaacaacga ccgcggacct cgcaatgctg gtggaacaaa
    12421 atcgttttag tcgccagctg tattacgcgc tgcatgcatt tgaaattacc atcccgcctc
    12481 tgcgtatgcg gcgtggcagc attccggcgc tggtgaataa caaattacgc agtcttgaaa
    12541 aacgcttctc tacgcggctg aaaattgatg acgatgccct cgctcgcctg gtttcttgtg
    12601 catggccagg caacgatttt gaactttaca gcgtcatcga gaatcttgct ctgagtagtg
    12661 ataacgggcg cattcgcgtc agtgatttac ctgaacatct gtttaccgag caggcgacag
    12721 atgatgtcag cgccacccgc ctttccacca gtctgtcatt tgcggaagtt gaaaaagagg
    12781 caattattaa cgcagcccag gtcacaggcg gtcgcattca ggaaatgtcg gctttacttg
    12841 ggatcggccg cactacgctg tggcggaaaa tgaagcaaca tggcattgat gcagggcagt
    12901 ttaagcgccg ggtatgaaag acagaaacga tttctgatac atcagagtga tctgtatttc
    12961 attccggcgc acgctaacaa ttttcagcat cgtttaaggg cttgtctatc ccgcacttaa
    13021 aagctgaagc gatatcctcc gttgacttgc ttctgatcaa agaggttacc ctgcgtgtaa
    13081 tccgcttcga gatagaatgt gtgctgtttg ttatactgtg cactgactcc cacgccgtta
    13141 ttccagccat tacctttgaa actgtacttc tcccgggagt tgttcaacag atattcggta
    13201 tcccctgaaa actcgcggat agcgccagtc ttcacataga cattcagctg gctgttgcct
    13261 gcggtgatgt cataccccag tatcatgctg gcacgcccca gcagcgattc gtagtgattc
    13321 agatgtatat tgaggctatt actcgccttc atagccatct cattctggtg gctgtatgta
    13381 agctgggttt gcggctctat atagaaccca taaccagtag gggacaggtt gaacctctgc
    13441 tcggcttcca gggagatgct cattccattc gcagtgccgt tggcgttaac gccgttgttc
    13501 tgactgtcca gtacgtggaa actatttttc tggcgcgatg cttttataac gagatcgctg
    13561 taaaaaccgt tttgtgccat gtaactggcg tacattccca tgtagtctga acgtgcggta
    13621 ccgtcgcctc cgctatagtc cggcgatgca tgtgttgagc caatatacag accgacatac
    13681 aacggcatta catcagagag acgtttatcc ccaccaaact ggataccgct gtaacccatg
    13741 tcaaagccgc tcagtttgcc actggcaaag gagtccaggc ttcccccata actgcgcaac
    13801 cagatattac cgtctttact ctgatttcgc aggtcaccca tccgttgcat cagcgtacgg
    13861 ttttcaacat agttcaataa gtaaccgaca ttgagataat tgccgccagc atctgcggtc
    13921 gtggtgggtt taggcgtggg agcgggttca ggcgtaggat cggggttgac tatgggaggc
    13981 tgagcgggag ctggtgtggg ttcaggatta ggagtcggtt ccggaactgt cccggaagcg
    14041 taaagctccc agttagtgcc atttttacgc acatcgtaca gatatccccc caactcaacc
    14101 tgagaagacg cgctgaacga ggccgcaccg tcagtggttt ttaccactgt cagaacttca
    14161 tttcccgtag tggcctcgct gccctggttg cggatagcca atacgtgatt accagcacta
    14221 cttccgctga tattcaataa atccccttta ttattaacgc cattaccctc gccaacaaca
    14281 tcagcacgca taataaaggt actgttaccg ctcaggttct ctacgtttaa tgtggcaaat
    14341 gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg aatggctcag cgccagcgtg
    14401 tcgagattag aattacttgt tacgttccag acgctgttat tcattgcaac gtccagtttc
    14461 ccgccattga cattatcgct gagggaggaa cctgtccaaa ccgaccctgg gtgcatatct
    14521 agattgatta gcccaccttt ggaaagaacg ctaccgttga taagcatacg acccgaggcg
    14581 ttgatgcggc tggcggcata accatcgtca tgttgcgttg caatggccat ctggtcgggt
    14641 gtactcatat caatgaccag atcgctcgtg aggtcgatct ggctgttggt catggcataa
    14701 attcccctgg ctcctgccgc gccggtgata gccaaactgt ctccggttat tctaccgccg
    14761 ctgagcgccc acaaacccag cgccagactg ccattacgat caactgtaat atcggtattt
    14821 tgcatgttga caacagccgt tgccgtctgg gccgaggcac cataagaacc gccggaaaag
    14881 atgctgtttc gttgcgctgc cgtgccagaa aaattgattg tcgcgtctga accactggtg
    14941 acgaggccac cgccctgcgc ggaagaaata tggctatctg caccgatagt ggttgtacca
    15001 ccacgaactt cgacgccatt ggccgcggct ccagttacat caacagtgag tgcattcgcg
    15061 ctaacctggc caaagctcca gaggccgtgt gcattatcgc cattggtttt aatggtactg
    15121 tttgttccga gatcgacaac agagtttttc tgtacgttta tccccatggc gctgtagccc
    15181 tgaacatcga ttgtcaggtc tgtcgccgta aaacgcgcag caccattggc gttattgccg
    15241 ttgagaccac cgatataaac acctgtactt ccatcggtcg tgattttact tccgcttcca
    15301 agatcgacac tggtgccata gtcattgatg gttaggccta taccgttcga gttttcaatg
    15361 gtgaattgag tggctgttag tgttgagcta tgcccaataa tgatgccgtc atcgttactc
    15421 ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt caccaattaa gttaatgccg
    15481 atggcagagg tttgaccaac aacatctact gtgagtcggt tagctgtcag ggagctgttt
    15541 ttgccctgaa taatgattcc ttttgcatat ccagaggcgt cgttgacatt gacggtgaca
    15601 tcattaccca ggttgatatt gccaggcgtg tttcctgctg gggtcattac accatagagg
    15661 tttcctgatg gatcggcggt atctcctgta attctgtcac catcattaat agtaatattg
    15721 gtgccatatt gacttcctgt atcagcagct atcagcaggg gggaaaagat aattgcattc
    15781 aaaccaatag cgccaaattt tatcagttga attgagggcg aacggatttt taattcagcg
    15841 agacgatctg ctttggtatt cccattgtgt tgtttgatgc ccataccact cctatatagt
    15901 acccagacta tgggtctatg gattttgtca ttttgtttaa atcaatgaat aatatcctct
    15961 tatcatcatg atatttgtat ggtgcaatca tgatgagctt ctcagagaat aattctctat
    16021 taccgggcaa aatcaggaac acaggaatta atatatattt aacatccccc ttacaaaggt
    16081 gggtttatat cgagttattc cctaagttgt cgttgcttat tccaattcgt tactataaat
    16141 atggcaaaaa tattacaaca aacagcggtt aaaaaatttg gtgaagattt tttgtaataa
    16201 tcctaattag ttatgggaat ttcgcttgat gtatctgcat aacaaaaaca atatgcgcca
    16261 tttttgcatt actaataaga aaaagcaaaa aacatcgata ttcatttttg gtcaatgggt
    16321 tttctggcct atcgttgtta ttgtgctact ggttatcatt ttcatcttgc caggatcgca
    16381 cgtaatcacg ttattcgggg actttatctg cgggatatta cccttcaggt aagtcctttt
    16441 ctcgccagca tattggagcg tgatcaatat tgatcagctg tgaagggggt gatgtgtagt
    16501 ggaccaaaac ttacgttata tcactactaa tgagatatcc gtcatctgac actgatgtct
    16561 ttaattattc tcgtggttca ctgtaggcaa taagcacaaa agtgtaggat gttacaagaa
    16621 tgattaggac tcggtgaaat gaaaaatcca cgcaattgcg tggattatat atacttttgc
    16681 gctcttcatg agatttagcg aaacctcatg agacaacaaa ttaattagac gttgaaaagg
    16741 aagttcatca catcgccatc tttaacgatg taatctttac cttctgcacg cattttgcct
    16801 gcttctttcg cgccttgttc acctttgtaa gtgatgaaat cttcaaacga gatggtttgt
    16861 gcacggataa agcctttttc aaaatcagta tggattttgc ccgctgcctg cggcgcggtt
    16921 gctccaaccg gaatggtcca tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc
    16981 aggttcagca gcttataacc ggcacggatc acacggttca ggcccggctc ttccagccca
    17041 agctcctgca taaactcgtc acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca
    17101 gcagcacaaa ccggaaccac aacagaacct tctttcgccg cgatttcacg cacctggtca
    17161 agatatgggt tgttttcaaa accgtcttcg ttgacgttgg cgatgtacat tgttggtttc
    17221 agcgtcagga agctcaggta acgaatagcc gctttctctt cagcgcttaa atccagcgcg
    17281 cgcagcatac ctgcattttc caactggggc aggcattttt ccaggaccgc cagctcagct
    17341 ttcgcgtctt tatcgccacc tttggctttc ttctgtacgc gatgaatcgc acgttcgcag
    17401 gtgtcgaggt ctgccagcgc cagttcggtg ttgataactt caatatcgtc agccgggtta
    17461 actttgccgg aaacgtgaat gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc
    17521 gcttcggttt cacggatgtt ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg
    17581 ccttttacca gaccggcgat atcgacaaat tccatggtcg tgggaagcgt acgttgcggt
    17641 tttacgattt cagccagttg atccaggcga gggtcaggca ttggtacgac gcctgtgttc
    17701 ggctcaatgg tgcagaatgg aaagttggcc gcttcaatac cggctttggt cagcgcgttg
    17761 aacagggtag atttcccgac gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt
    17821 taaatcacct taatatctta ataatcaacc tgttattgct aacagattgc agaaatggaa
    17881 ataactttgc ctattataca cggcactcgg caaaaatgcc gcagacaacg acttattgcg
    17941 ctttaaaggc gtgcaatcgg ttcgttgctt tggtcaagcc atctgtaaac cacatttcag
    18001 tacaacgcgc cgcttcgtcg atggcttcat caattaactt ctgttcacta acaggcggtt
    18061 tgcctaacac aaaaccgaca actttatttt tatcgcccgg atgaccgatt ccgatgcgta
    18121 aacggtgaaa gttagggtta ttacccaatt tactgatgat gtctttcagt ccattgtgac
    18181 caccatggcc accgcccaat ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt
    18241 gggccaccag aatttcgtcc ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt
    18301 tgccgctgag attcataaat gtagtcggga ctaacaggcg gacatcttcg cctccaagag
    18361 tgactcgcga agtataacca aagaatttag cctcttcgcg cagcggagcg cgcaaacgct
    18421 ctgccagtaa gtcaacgaac caggcaccag cattatgtcg cgttgcggcg tattcagcac
    18481 cggggttcgc caggccgaca atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac
    18541 ataactggcg cgtagtttac tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg
    18601 cagataacgt aataattgca tgagtggact attagaaagt caaggtgttc aggcgtttat
    18661 ttgtaaagtt ttgttgaaat aagggttgta attgtgatca cgcccgcaca taacccacag
    18721 ggtgttgtct atactttaca cataaggaag aggggtattc cctgttacaa cccagaaagt
    18781 tccggaggtg acatatgaaa cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt
    18841 tgggtctggt ggtaatggtg gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt
    18901 ttaatatgcc ccagtatttc gcacatggtg cagtgctaag tattttcgtc ggtgccattc
    18961 tctggctggc gggtgcccgt gttggcgggc atgaacaggt gtgcgaccgt tactggtggg
    19021 ttcgccacta tgacaaacgt tgccgccgta gcgataatcg ccgtcatagc taacaaataa
    19081 tgcagtttgc tgaccagtca gtttgcgctg actggtcaat ctcgtactta taaatccgcc
    19141 atcgccgcgc gacgattcgg gaagaacgcc aggcgtcccg ggatcggttg aatgccagcg
    19201 cgagccatag tgcgcagtgg ctggaattcc acgttgcaca cgcgcagttc acatccctcg
    19261 ggcagacgct tcacaaaacg ctggaacgca tcaagaccac cagcatcaag taccggaacc
    19321 gcatcccact tcagaatcac aatccgtttg ccttcaagac gtgactccag gtccgtgaac
    19381 aagccttcag cagcagcaaa aaacagcggg ccaataacgc gcagaaccag gacatcgtct
    19441 ggaacatcta cgactaccgg tgccaggcga gtcatacgtg cgatacgacg cataaacagc
    19501 agcgatgcca gcacgatccc cacgctgatg gcaataacca tatcaaacag cacggtcagc
    19561 gacatgcaca gcagcatgac aatgatgtca tctttcggcg catgacgcag caagtcgacc
    19621 actttgtgcg cttcactcat gttccacgcc accatcaaca gcagggcagc catggcggaa
    19681 agcggcagcc aggagagcag cggtgccagt accagcaggg caagaataac cagaatagag
    19741 tggatcaccg ccgagatagg ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg
    19801 atggcagctg tagcggtaat gccaccaaag aacggagcga tgatattccc cagtccctgt
    19861 ccaaccagtt cgctattcgc cttgtgtttc gtcccggtca taccatccag caccacggcg
    19921 cagagcagag attcgattgc gccgagcatt gccattgaga atgccgcagg cagcagtgtg
    19981 cgaatagaat cccaggttag cgtgaattct gaattaggca gatcccacgg cagcaccagt
    20041 tgcggcagca gttgcggaat accgttaccc tgagaaccat cggccaggac gtagtggaat
    20101 tgcgaaccga tggtagcaac atgtccgccg agcaggttaa caatccccat caccgcgcaa
    20161 ccagccagca aggccggaag gtgaccgggt aaacgaatgc ccagacgcgg ccaaaaaaca
    20221 agaataccta gcgtcacaat gccaatggca gcatcaccca cattaatggt cggcagcgcc
    20281 ataaataatg cgccgacttt ttgtagataa tgttccggga catgggccat ttgcagaccg
    20341 agaaaatctt taatctgcat ggtaccgatg gtgatcccga tacccgaggt gaaacctaag
    20401 gtgacggaaa ccggaatata ctcaatcagg cgaccaaagc gtgccagacc catcagaatc
    20461 aaaaaaatcc ccgacagcaa ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgac
    20521 acgggataga gaattaccac aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca
    20581 cccgtcagag caatgataat ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc
    20641 acaccactac caatagccaa cgccatcgcc agcgggatag caataatccc gacggttatc
    20701 ccggcaatca ggtcacgggt aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg
    20761 atcagagcgc ggaaaggcat cacatgtgag gaaaatattt tgttcacaat aatgtttcat
    20821 ccgtgagcgc atcatctgtc aactaaatgg caggtgaagg aggcataggt catacaaatg
    20881 gatattacag acaaaaaaac ccgccgcagc gggtctttga gccgggttcg attagtgttc
    20941 gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg ccagcatacc
    21001 tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca gcggaatggt
    21061 atcgcagaca acgacttcat caattacaga gttacgcagg ttattcgccg cgttgccaga
    21121 gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt tcagagcttc
    21181 agcagcttta cacagcgtac cgccagtgtc gatcatatca tcgaccagta cgcagtcacg
    21241 acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt
    21301 gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg cacgcacaac
    21361 gccgccgatg tccggagaaa ccacaattgg gttatccaga ttcagctgca gcatgtcttc
    21421 cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac cctgaatctg
    21481 ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga ggaagtctgc
    21541 aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct ggcgcgcata
    21601 gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca gggcatcaac
    21661 cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact ggatgatgaa
    21721 aatatcacca ccgcgtacat tttcgttgat ttgtacactg acttcgccgt cgctaaagcg
    21781 acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt gtgctagttc
    21841 cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct caggcatgcg
    21901 tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg
    21961 gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc gaaactcagc
    22021 ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa
    22081 gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact ctgtatcaaa
    22141 ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca
    22201 ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct
    22261 gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg ggagttcagg
    22321 atctttaaaa atcaccggag tcggaatact tacaccaggg tgcgccacca gataccactt
    22381 ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg cggcatgccc
    22441 ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc atttccgcca gctcatccat
    22501 gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg cattggatga
    22561 accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat tcgcaccgct
    22621 tcccgtcgga agacgcccgc tgtctgccgc agtttttatc aacaatcgcg ctgcgcgaac
    22681 gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa tatccccatc
    22741 gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag
    22801 cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa gttttgccgg
    22861 agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa cttgatgcgt
    22921 tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgcgtttt ggtgtcataa
    22981 ccaccataaa caaccttcca gtttttgcca ttctggctgt aggtaatttc gctcaggcgg
    23041 tactggtcgt ccagtttgta gtcggttgca tcacccggta aacctaaaat ccactggcgc
    23101 aagctgttga gcggaattgg cattccggtc aatttgccaa tcatctcttc ggcgtcatcg
    23161 gcggtataac gctgaccttt attgtcgact aactgcacgt tacccggttg agcattcagc
    23221 tccagttccg tgctgcccaa tgggttagtg agcagcagac ggtagcgatc ctggccggtt
    23281 tgctgccaga aaaagcgggc gtacactttt tgttggtcag aaatataagc gaacgcgccg
    23341 cgagtctgat actgattaag attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa
    23401 tccgggcttt tgccaggacc tttgggcgtg gtaacggaac aggcagtgag cacaagagca
    23461 gccagcggta gcaggcggat aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct
    23521 tgagatacgt tgcagttata acccttaatg ctagcgttac cgtccgctat cgtctatgtt
    23581 caagttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa agggggcgct
    23641 ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaacccta tcaacgttgg
    23701 tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa acggcacctg
    23761 tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg cttgacagcc
    23821 tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac
    23881 tttatcttag cgttgaagag caggataacc tgcaagaggc gttaatccgc tggctttgcg
    23941 attatcacaa tcttaatgaa gaagatctgc gtaaaagcct ctactggcat caggataacg
    24001 acgcggttag ccatttaatg cgtgttgcca gcggcctgga ttcactggtt cttggggagc
    24061 cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg
    24121 ccagcgaact ggaacgcatg ttccagaaat ctttctctgt cgcgaaacgc gttcgcactg
    24181 aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc
    24241 agatctttga atcgctctct acggtcacag tgttgctggt aggcgcgggc gaaaccatcg
    24301 agctggtagc gcgtcatctg cgcgaacata aagtacagaa gatgattatc gccaaccgca
    24361 ctcgcgaacg tgcccaaatt ctggcagatg aagttggcgc ggaagtgatt gccctgagtg
    24421 agatcgacga acgtctgcgc gaagccgata tcatcatcag ttccaccgcc agcccgttac
    24481 cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag ccgtcgcaac caaccaatgc
    24541 tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg
    24601 cttatcttta tagtgtggac gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta
    24661 aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa tttatggcgt
    24721 ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag gcagagcacg
    24781 ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc gacgcgcaag
    24841 ccattatgca ggatctggca tggaaactga ctaaccgctt gatccatgcg ccaacgaaat
    24901 cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg cgcgacagcc
    24961 tcgggctgga gtagcagtac atcattttct ttttttacag ggtgcattta cgcctatgaa
    25021 gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag ttcaggcgtt
    25081 gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat cacgcgaata
    25141 tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc aggaagatat
    25201 cgaaaccgca cagatgatgc tcgatgatcc tgaaatgcgt gagatggcgc aggatgaact
    25261 gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc tgttactgcc
    25321 aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgagccggaa ccggcggcga
    25381 cgaagcggcg ctgttcgcag gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg
    25441 ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa catggtggtt ataaagagat
    25501 catcgccaaa attagcggtg atggtgtgta tggtcgtctg aaatttgaat ctggcggtca
    25561 tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt ctgcttgtac
    25621 cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc cagcagattt
    25681 acgcattgat actttccgct cgtcaggggc gggtggtcag cacgttaaca ccaccgattc
    25741 ggcaattcgt attactcact tgccgaccgg gattgttgtt gaatgtcagg atgaacgttc
    25801 acaacataaa aacaaagcga aggcgctttc agtgctcggt gctcgcatcc acgctgctga
    25861 aatggcaaaa cgccaacagg ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga
    25921 tcgcagcgac cgtaaccgta cttacaattt cccgcagggg cgcgttaccg atcaccgcat
    25981 caacctgacg ctctaccgcc tggatgaagt gatggaaggt aagctggata tgctgattga
    26041 accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc aggaataatg
    26101 gaatatcaac actggttacg tgaagcaata agccaacttc aggcgagcga aagcccgcgg
    26161 cgtgatgctg aaatcctgct ggagcatgtt accggcagag ggcgtacttt tattctcgcc
    26221 tttggtgaaa cgcatctgac tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt
    26281 cgtcgcgatg gtgaacccat tgctcattta accggggtgc gagaattctg gtcgttgccg
    26341 ttatttgttt cgccagcgac cttaattccg cgcccggata cggagtgtct ggtggagcag
    26401 gcactggcgc ggttgcctga acaaccttgc cgtattcttg atctcgggac gggtaccggg
    26461 gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg aaattatcgc tgtagatcgt
    26521 atgcctgatg ctgtctccct ggcacaacgt aatgcccagc atctggcgat caaaaatatc
    26581 cacattctgc aaagcgactg gtttagcgcg ctagccgggc agcagtttgc gatgattgtc
    26641 agcaatccgc cgtatattga cgagcaggac ccacatcttc aacaaggcga tgtccgcttt
    26701 gagccgctca ctgcgctggt tgcggcagac agtggaatgg cagacatcgt gcatatcatc
    26761 gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc ttctggaaca tggctggcag
    26821 cagggcgaag cggtgcgaca agcatttatc ctcgcggggt atcatgacgt cgaaacctgc
    26881 cgtgactatg gtgataacga gcgcgtaacg ctcggccgct attatcaatg acaagttttt
    26941 ctacactgct tagtgttcat cttattagtc tcgcgctttc tgttgggcta ttaaccttac
    27001 gtttctggct acgttatcag aagcatccac aggcatttgc tcgctggacg cgcattgtgc
    27061 cgccggttgt cgatacggtg ttattgttaa gcggcattgc gttgatggct aaagcgcaca
    27121 tcctgccatt ttccgggcag gcacagtggc tgactgaaaa gctgtttgga gttatcattt
    27181 atatcgtttt gggttttatt gcactcgatt atcgtcgtat gcacagtcag caggcgcgca
    27241 ttattgcctt cccgctggcg ttggtggtgc tgtacatcat cattaaactc gccaccacaa
    27301 aagtaccgtt actggggtaa gtcatgaggt cgttagctga tttcgaattt aataaagcgc
    27361 cattgtgcga aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag
    27421 atgtttacga cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc
    27481 tgcctttaga agagcagctg gaaaaactga tcgcgctgtt ttacggcgac tggggattta
    27541 aagcctcacg cggcgtttat cgtctttccg atgcattatg gctggaccag gtgttaaaga
    27601 atcgacaggg cagtgcggta tcattaggtg cggttttatt atgggtcgcg aatcgtctcg
    27661 atttgccgct gctgccggtg attttcccta cgcagctgat attgcgcatt gaatgtccgg
    27721 atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa catatgctgg
    27781 acgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa gaccttgatg
    27841 aagctgataa cattgaggta atccgcaaat tgctggatac actcaaagcc tcgttgatgg
    27901 aagaaaatca gatggagctg gcgttacgca ccagcgaagc tttattacaa ttcaaccctg
    27961 aagatcccta tgaaattcgc gatcgcgggt tgatttatgc gcaactggat tgcgaacacg
    28021 ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc ggaagacccg atcagcgaaa
    28081 tgatccgtgc gcaaataaat aacatcgcgc ataaacatat tgtgctgcat taattaatcg
    28141 acattttact caagattaag gcgatcctat gaaacaaaaa gtggttagca ttggcgacat
    28201 caacgtagca aatgacctgc cgttcgtact gtttggcggt atgaacgtgc tggaatctcg
    28261 cgatctggcg atgcgcattt gcgagcacta cgtaactgtg acccagaaac tgggtatccc
    28321 ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc tccatccact cttatcgtgg
    28381 accgggcctg gaagaaggga tgaaaatctt ccaggagttg aagcagactt ttggcgtgaa
    28441 aattatcacc gacgttcacg aaccaagtca ggcacagccc gttgctgatg tcgtggatgt
    28501 gattcagttg ccggcgtttc ttgctcgcca gactgacctg gttgaagcca tggcgaaaac
    28561 cggtgcggta attaacgtca agaaaccaca gtttgtcagc ccgggacaga tgggtaatat
    28621 cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc gtggtgctaa
    28681 cttcggctat gacaacctgg ttgtcgatat gctgggcttc agcattatga agaaagtgtc
    28741 tggtaactcg ccggtgattt tcgacgtgac ccacgcactg caatgccgcg atccgtttgg
    28801 cgcagcttcc ggtggtcgtc gtgctcaggt ggctgagctg gcacgagccg gtatggcggt
    28861 aggtctggcg gggctgttta ttgaagcgca tccggatccg gaacatgcga aatgtgatgg
    28921 tccatccgcg ctgccgctgg ctaaactgga accgttcctc aagcagatga aagcgattga
    28981 tgatctggtg aaaggtttcg aagaactgga taccagcaag taatcttttt tgcttgaaaa
    29041 ataaagtctt agcgttctgc gttaagactt ttttcatggg tgccagatac aaaaa
//
LOCUS       DAHBHC010000065        28705 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHBHC010000065 DAHBHC010000000
VERSION     DAHBHC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.065"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(239..1006)
                     /gene="yafV"
                     /locus_tag="M2A35_003391"
                     /pseudo
     CDS             complement(239..1006)
                     /gene="yafV"
                     /locus_tag="M2A35_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
     gene            1187..1633
                     /gene="ivy"
                     /locus_tag="M2A35_003392"
     CDS             1187..1633
                     /gene="ivy"
                     /locus_tag="M2A35_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCB7199645.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1676..4120)
                     /gene="fadE"
                     /locus_tag="M2A35_003393"
     CDS             complement(1676..4120)
                     /gene="fadE"
                     /locus_tag="M2A35_003393"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCB7199646.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4360..4938
                     /gene="lpcA"
                     /locus_tag="M2A35_003394"
     CDS             4360..4938
                     /gene="lpcA"
                     /locus_tag="M2A35_003394"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCB7199647.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5144..5911
                     /locus_tag="M2A35_003395"
     CDS             5144..5911
                     /locus_tag="M2A35_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCB7199648.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5882..6622)
                     /locus_tag="M2A35_003396"
     CDS             complement(5882..6622)
                     /locus_tag="M2A35_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCB7199649.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6778..7056)
                     /gene="yafQ"
                     /locus_tag="M2A35_003397"
     CDS             complement(6778..7056)
                     /gene="yafQ"
                     /locus_tag="M2A35_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCB7199650.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7059..7319)
                     /gene="dinJ"
                     /locus_tag="M2A35_003398"
     CDS             complement(7059..7319)
                     /gene="dinJ"
                     /locus_tag="M2A35_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCB7199651.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7505..8278
                     /locus_tag="M2A35_003399"
     CDS             7505..8278
                     /locus_tag="M2A35_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCB7199652.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(8335..8691)
                     /locus_tag="M2A35_003400"
     CDS             complement(8335..8691)
                     /locus_tag="M2A35_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCB7199653.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(8684..8962)
                     /locus_tag="M2A35_003401"
     CDS             complement(8684..8962)
                     /locus_tag="M2A35_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCB7199654.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9067..10779)
                     /locus_tag="M2A35_003402"
                     /pseudo
     CDS             complement(9067..10779)
                     /locus_tag="M2A35_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10751..11536
                     /gene="lafU"
                     /locus_tag="M2A35_003403"
     CDS             10751..11536
                     /gene="lafU"
                     /locus_tag="M2A35_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCB7199655.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPKSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            11607..12662
                     /gene="dinB"
                     /locus_tag="M2A35_003404"
     CDS             11607..12662
                     /gene="dinB"
                     /locus_tag="M2A35_003404"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCB7199656.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12659..13111
                     /locus_tag="M2A35_003405"
     CDS             12659..13111
                     /locus_tag="M2A35_003405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7199657.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            13357..14496
                     /locus_tag="M2A35_003406"
     CDS             13357..14496
                     /locus_tag="M2A35_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCB7199658.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            14493..15107
                     /locus_tag="M2A35_003407"
     CDS             14493..15107
                     /locus_tag="M2A35_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCB7199659.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15164..16621)
                     /gene="pepD"
                     /locus_tag="M2A35_003408"
     CDS             complement(15164..16621)
                     /gene="pepD"
                     /locus_tag="M2A35_003408"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCB7199660.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            16882..17340
                     /gene="gpt"
                     /locus_tag="M2A35_003409"
     CDS             16882..17340
                     /gene="gpt"
                     /locus_tag="M2A35_003409"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCB7199661.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17432..18676
                     /gene="frsA"
                     /locus_tag="M2A35_003410"
     CDS             17432..18676
                     /gene="frsA"
                     /locus_tag="M2A35_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCB7199662.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            18734..19135
                     /gene="crl"
                     /locus_tag="M2A35_003411"
     CDS             18734..19135
                     /gene="crl"
                     /locus_tag="M2A35_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCB7199663.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19174..20229)
                     /gene="phoE"
                     /locus_tag="M2A35_003412"
     CDS             complement(19174..20229)
                     /gene="phoE"
                     /locus_tag="M2A35_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCB7199664.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            20517..21620
                     /gene="proB"
                     /locus_tag="M2A35_003413"
     CDS             20517..21620
                     /gene="proB"
                     /locus_tag="M2A35_003413"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCB7199665.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            21632..22885
                     /gene="proA"
                     /locus_tag="M2A35_003414"
     CDS             21632..22885
                     /gene="proA"
                     /locus_tag="M2A35_003414"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCB7199666.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            23000..23075
                     /locus_tag="M2A35_003415"
     tRNA            23000..23075
                     /locus_tag="M2A35_003415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23033..23035,aa:Thr,seq:cgt)
     gene            23682..23840
                     /locus_tag="M2A35_003416"
     CDS             23682..23840
                     /locus_tag="M2A35_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199667.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
     gene            complement(24292..24831)
                     /locus_tag="M2A35_003417"
     CDS             complement(24292..24831)
                     /locus_tag="M2A35_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7199668.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLSYLREGDTLHVHSLDRLGRNTLDILALVEELNNRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGNGKAVDREGIKKALSDGLSVRKTAE
                     IFNVAPSTVQRIKKEEEEA"
     gene            26769..26957
                     /locus_tag="M2A35_003418"
     CDS             26769..26957
                     /locus_tag="M2A35_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199669.1"
                     /translation="MKWFTPKHVVEAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
     gene            26977..27147
                     /locus_tag="M2A35_003419"
     CDS             26977..27147
                     /locus_tag="M2A35_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199670.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            27201..27890
                     /locus_tag="M2A35_003420"
     CDS             27201..27890
                     /locus_tag="M2A35_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7199671.1"
                     /translation="MINQLTFTKHYDTFDNVSKIYSDKFPQGKDLDLLHIVLYFRFLS
                     YQENNLNCYESHETLAKIFKSSASTIKRKINDLKEMGLLETSPHPDPYISSLIYNALP
                     LTDAHITPPGESSLSDLSEAAEAQEQPKGHKQPSFDVLDDWDAPLPWETEETPVSSSE
                     KVANDNEEDVLENFASLICQQKHTRTGGASFIEFANSLAYRHGLKRPNGIEAYFSKNY
                     PNVYDDFDIPF"
     gene            27902..28180
                     /locus_tag="M2A35_003421"
     CDS             27902..28180
                     /locus_tag="M2A35_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199672.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIVFIYSTL"
     gene            28313..>28705
                     /locus_tag="M2A35_003422"
     CDS             28313..>28705
                     /locus_tag="M2A35_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199673.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLSKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFHLDNDDH"
BASE COUNT         7415 a         6952 c         7021 g         7317 t
ORIGIN      
        1 agcgggcttt cgtaatcttg ccctggcgtt aaacacatgt tatatagaag aagtgcttgc
       61 atcctgctca gagcttgggt ttcgaactga caaaatgaaa aatttaacac aaatttaatt
      121 catgctcttg ccctgcgggg ggattattaa atcttcccag ttttttatta tttaactctc
      181 gctcagcaca ctaaacatcc ctttctgaat tccccgccct aaatacagcc cccactcact
      241 acaacctaaa ctcatccgca tcccgccatg ccggaaactt atcgcgatat tcccttaaca
      301 ccgccatcga cagctcggca tcaatgcgcg ttgcctgatg cgcgtcggca gtagcgataa
      361 tctcgccttg tggattaatc acccggctgt caccgcgata atggcagccg ttgccatcgc
      421 tgccgacgcg attgcatccc gccacatacg cctgattctc aatcgcgcgg gccgtcagca
      481 atgcctgcca gtgcagagag cgcggagcag gccagttggc gacgtacagg gcgaggtcat
      541 aatcgttgag attgcgcgac cacacaggaa aacgtaagtc gtagcacacc agcggcaaaa
      601 tacgctagcc gcgccattcc acaatcactc gcgcattgcc cgctttataa tgtagatgct
      661 catctgccat gcggaacaga tgacgcttat cataaaaatg taccgtgccg cccggctcaa
      721 ccagcaaaaa gcggttaacc gaaccagact ccgtttgtaa tgcaacactg cctgcaatca
      781 gcgcattgca ctgctgcgcc ttagctgtca tccagttcac tacgtcatct tgtgctagcg
      841 acgaagctgc cgcttccatg gcaaagccgc tggtaaacat ctccggtaga acgatcacat
      901 cgcgcccggt aataccttcc agttgacgat caaaatgacg caggttggca gggccatcca
      961 tccacaccag tggttgctgc aaaagcgtaa tcttcaaacc aggcacggtg tacaactcct
     1021 ttatgcgaag ggttttataa ctttaacacc ttatcaggca gttgccttag cgcagaataa
     1081 attgataaca aatgctgata ttggaaatat ctgatttgca aattatcgtg ttatcgccag
     1141 gctttagaag gttaataaca tgggcaggat aagctcggga ggaatgatgt ttaaggcaat
     1201 aacgacagtc gccgctctgg tcatcgccac cagtgcaatg gcgcaggatg atttaaccat
     1261 tagcagcctt gcaaagggcg aaaccaccaa agctgcattt aatcagatgg tacaagggca
     1321 taagctgcct gcctgggtga tgaaaggcgg tacttatact cccgcacaaa ccgtaacgtt
     1381 gggagatgag acgtatcagg tgatgagcgc gtgcaaaccg catgactgtg gctcgcaacg
     1441 tatcgctgtg atgtggtccg agaaatctaa tcagatgacg gggctgttct cgactattga
     1501 tgagaaaacg tcgcaagaga aactcacctg gctgaatgtg aacgatgcgc tttcgattga
     1561 tggtaaaacg gtgctgttcg cggcgttgac cggcagcctg gaaaaccatc cggatggctt
     1621 taattttaaa taattagcgg ataaagaaac ggagcctttc ggctccgtta ttcaattacg
     1681 cggcttcaac tttccgcact ttctccggca actttaccgg cttcgtcgcc agctcttccg
     1741 gatcaaagtc atcaacgtta atactgcgca gacggctttc ttcagctttc accagaatag
     1801 cggcttcatc tttatcaatc agccccttcg ccagcgcgtt gtgcgccagt tcatccagac
     1861 gggtaaacgg caggttttta cccagctctt tacagatccg ctgatgaatt gggtcggcgg
     1921 caatcacatc caccagcgcc tcttccagca agccaaccgg attatgctcg ctcggcgtca
     1981 ggtactgacc gcgaccaatg cgggaacggg tggcgttcgg cacttgtaaa atcttcgcca
     2041 ctttatgatc cagcttgtca gaaggtgcca gataatgacg tccggtcggg aagatcacca
     2101 cattcagcag cccggcaacc acgcggttcg ggaagttttg cagtaaatca tccatcgcct
     2161 gttcagcctg atacagcgca tcttgtacgc cccagtgcac cagcggcagg tcggcttcat
     2221 tacggccttc gtcgtcataa cgcttcagaa cggcagaggc gaggtaaagc tggcttaaaa
     2281 tatcccccag acgtgccgag atgcgctcac gacgtttcag gctgccgccc agcacagcca
     2341 tcgagacatc agaaagcagg gcgaggttgg cgctcaggcg gttcaggtgc tgatagtagc
     2401 gtttagtggc atcgccggtt ggcgtgctgc tggttaaacc gcgcgtcagg cccagccaga
     2461 agctgcgaac tttgttgcta ccgacgtgac cgatatgttt gaacaacagt ttatcgaacg
     2521 cgttgacgtc attgttcttc gctgcttcca tctcttccag cacgtacgga tggcaacgaa
     2581 tcgctccttg tccgaagatc atcatgctgc gggtcagaat gttggcccct tcaacggtga
     2641 tggcaatcgg tgcgccctgg taagcacgcg ccaggaagtt gctctgcccg agcataatgc
     2701 ctttgccacc agtgatatcc atcgcatcaa taatcgactg ctgcccgcgg tgggtacagt
     2761 gatacttaac gatagccgac agcacggcag gtttttcgcc gagcataatg ccgtaggtaa
     2821 tcagcgatgc cgcagcatcc atcacgtagg cattaccggc aatacgcgcc agcggctctt
     2881 caatcccttc catcttaccg atagagattt tgaactgacg gcgaatgtga gcatacgcgc
     2941 ccgttgccag cgctaccgat ttcacgccgc cggttgagtt ggaaggcagg gtgatgccgc
     3001 ggcctaccga gaggcactcc accagcatcc gccagccttg cccggccatt ttcggcccac
     3061 cgatgatgta atcgatcggt acgaagacat ctttaccgcg cgtcggtccg ttctggaacg
     3121 gtacgttcag cgggaagtgg cgacgaccaa tttccacgcc cggcgtggtg gtcgggatca
     3181 gcgcacaggt aatacctaaa tcttctgcgc cacccagcag cttttccggg tcggagagtt
     3241 taaacgccag cccaagcaca gtcgcaatcg gtgccagcgt aatgtagcgt ttgttccagg
     3301 tcagacgcat ccccagcacc tgctggccct gccattcgcc catgcagaca atcccggtgt
     3361 ccggaatcgc gcccgcatcg gaacccgctt ccgggctggt cagtgcaaag caggggatct
     3421 cctgaccacg cgccagacgc ggcagatagt gatctttctg ctcgtcagtg ccgtaatgtt
     3481 gcaacagttc gcccgggcct aatgagtttg gcacgccgac ggtaatcgcc aggatcccgc
     3541 tcacgccgga gagtttttgc agcacgcgag actgggcata agccgagaac tccagcccgc
     3601 cgtactcttt tttgatgatc atcgcgaaga aacgatgctc tttaaggtac gcccacaact
     3661 ccggcggcag atccgccagc tcatgggtga tctggaaatc attcgccatc cggcaggctt
     3721 cttctaccgg gccgtcgaga aacgcttgct cttcggcggt caggcgcggc tgcggatagt
     3781 tatgcagctt tttccagtcc ggcttgccct ggaacaagtc gccctcccac caggtggtgc
     3841 ccgcatcaat cgcttctttc tcagtgcgcg acatcggcgg catcacctta cggaaaccgc
     3901 gaaataccgg cgcggaaatc atcgacttac gcataggcgc aaagttaaat ggcacgagga
     3961 taatggccag aggcaccagt acccacgccg accacagacc agcaacgccg agggcggctg
     4021 tccaggcgag caaaatcaga ctgctgataa ataagctcac gcggtgatag aacaacgcgc
     4081 cgagcaggac aaccgtagcg agaatactca aaatcatcat aacgaaaagc cccttacttg
     4141 taggaggtct gaccacttgt gatgatatgg ttgtagtgga tgtaaaaaca tttagcaatg
     4201 tgtttacaat ataattacaa caaagctcac attgttgctg tttttatccg cacttcaggt
     4261 caaaaagtcc tggtcatagc acctgcccgt acttctcgct tttggcggta tccggtacac
     4321 tgcattttgt ctattacatt tatgctgaag gatatcctca tgtaccagga tcttattcgt
     4381 aacgaactga acgaagcggc ggaaacgctg gctaactttt taaaagatga cgccaatatt
     4441 cacgccattc agcgcgcggc ggtcctgtta gcagacagct ttaaagccgg tggcaaagtg
     4501 ctttcctgcg gcaacggcgg ttcccattgc gacgctatgc actttgccga agagttgacc
     4561 ggtcgctacc gtgaaaaccg tccgggctac ccggcgattg ctatttctga cgttagtcat
     4621 atttcctgcg tcggtaatga tttcggtttc aatgatattt tctcccgcta cgttgaagcg
     4681 gtaggtcgcg aaggcgatgt actgctgggg atctccacct ccggtaactc tgcaaacgtg
     4741 atcaaagcga tcgcagcggc gcgtgagaag gggatgaaag tgatcaccct gaccggtaaa
     4801 gacggcggca aaatggctgg cacggcggat atcgaaattc gcgtaccgca ctttggttat
     4861 gccgaccgca ttcaggagat tcacattaaa gtgatccata tcctgatcca gttaattgaa
     4921 aaagagatgg ttaagtaagt ctggcgtagg ctggataagg cgtttacgcc gcatccggca
     4981 cttgtgcgct gatgcctgat gcgatgctga cgcgtcttat catgcctaca aatctgtacc
     5041 cgaaccgtag gccgaataat tcgtttacgc cgcatccgac ctgaaaattc ttaaatcaat
     5101 cttcgccggg ggccattcgc tcccgctgtt gtggaggtta cccatgtgcg aattgctcgg
     5161 gatgagcgcc aacgtcccta ccgatatctg ctttagtttc accggacttg tacagcgtgg
     5221 tggtggaacc gggccacata aagatggctg gggcattacc ttttacgaag gtaaaggctg
     5281 tcgcacattt aaagatccac aacccagctt taattccccc atcgccaaac ttgtccagga
     5341 ctacccgata aaatcctgtt cggtggtggc tcatattcgc caggctaatc ggggcgaggt
     5401 ggcgctggaa aatactcacc catttacccg cgagttatgg gggcgtaact ggacttatgc
     5461 ccataacgga caactgacgg gctacaaatc actggaaacc ggcaacttcc gcccggtcgg
     5521 tgaaaccgat agcgaaaaag ccttctgctg gctcctgcat aaattaacgc agcgttaccc
     5581 gcgcacaccg ggcaacatgg cggcagtgtt taaatatatc gcctcactgg cggatgaact
     5641 gcggcagaag ggcgttttca acatgctgct ttctgacggg cgctatgtga tggcgtattg
     5701 ctcgactaat ttacactgga tcacccgccg cgcgccgttt ggcgtggcaa cgctgctgga
     5761 tcaggatgtg gaaatcgact tcagctcgca gaccacaccg aatgatgtgg tcacggtgat
     5821 tgcaacacag ccgctgacgg gcaatgaaac ctggcaaaag attatgccag gcgaatggcg
     5881 cttattttgc ctcggggagc gtgtagtttg atgccagttg tggctgcaca acttcgtggc
     5941 ttaacggctt gctgaccacg taacggccat tgaccacaga aacggtcggt ggcttacggg
     6001 tttgctcaaa gtagtcgtag cccggcttca gctgctccca gaagtcctta aagttggaat
     6061 atttatggcg cttcatattg gcgtcggtca tgcggaacgg gtaaatactc acttgcacgc
     6121 tcggctgacc aaacaccagc gcaccagtaa cgaactggaa gatctcatca ataccctgat
     6181 tggtcattgc gtagcagccg atggaaacac aatcgccgtg gatcatcagg tatttccctt
     6241 cataaccatg cgcacggtca taggcattgg ggaaaccaat attaatcgct ttgtagtaac
     6301 ggctgtctgg ttttaactga ttacgctgga cgctataaaa cccttccggg cttttgaaat
     6361 cgccctgacg ctgttttggc cctaagccgc cggaatattt acagatttta taactgtcga
     6421 gcagctgata ttgctcgccc attttgacgt agagatcgag cgtacgttct tccttgaaga
     6481 tctggatgta gacaggggat cccatcaact gctgcttata ctctttgctc actggcgtgg
     6541 tcgagctact gctgcccagc aaaccggcaa acgaaacgca cgggatcaac aacatcgcaa
     6601 gaattaatgc gattttacgc atactgctta ttccttgata aaacggttac acacgccagg
     6661 acggcaaaat agatcccaaa tcggaatagt ctggatttgg aaggctcaca ttatcaccaa
     6721 aagagtttta cgcaagcctg tcggcgcagg gtttacaaat ttcatcggca gcgtgcttta
     6781 cccaaagagc gccgcgtgag ttccagttct ctcaaatcgt aaaagtttat cggtaagttt
     6841 gtaaatcagg atccagtccg gttcgacatg agcatcgcga taacctttcc atgaaccttg
     6901 cagcgggtgg tctttataaa cagctggaag cggtaaagca ttattgataa gaagcgtcat
     6961 aagatatttc aatttattca tatccttatg acgcttttgt gcaagtttta catcctttga
     7021 aaattgtccc gagtattcaa tatccctttg aatcatattt aaattcctaa tttatcaaat
     7081 aaatcatcgg cgtctttggc cttatgaaca tcaacgccag cttcgctgtt tttgattgat
     7141 tgaatggtta attgattagg ctcgcgtaaa tcaaacggca atgccttttc acgcgcgacc
     7201 tttgtgaggg ttatgcgaac caggtcagag atggtcagcc ccatcccggc cagtacgtcc
     7261 gccgcctgat tcttcagatc ttcatcgatt cgggcgcgaa caaatgcgtt agcagccatt
     7321 ggggtatctc ctgtgctgta tatttattca gcttgaattg tagctcaaat gagcaacagc
     7381 gacaatccat gagtaattta ataaacggat gtctttacat aattaactga aatatattta
     7441 ttaattttat aataatggtt tattaagttt gttcaggtac gggccgggat acactgcaac
     7501 atcaatgcat gatcagccat tattgagtat gtccttgccg tcgattccct cttttgtgtt
     7561 gtcgggatta ctgttgcttt gcttgccgtt ttcttcattt gccagcgcca ccacatcaca
     7621 tatcactttc agctacgccg cccgccagcg gatgcaaaat cgtgcgcgtt tattaaaaca
     7681 gtaccaaacc catctgaaaa aacaggccag ctatattgtg gaaggcaatg ccgaaagcag
     7741 aagggcgcta cgccagcaca accgcgaaca gataaaacag catccagaat ggtttcctgc
     7801 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc atttcttaag
     7861 cagcgaccat ctgcataaca tcaccgaagt ggcgattcac cgtctggagc aacagcttgg
     7921 caagccttac gtctggggcg gtacgcggcc cgatcaaggc ttcgactgta gcgggttagt
     7981 tttttatgcc tacaacaaga tccttgaggc gaaactccca cgcacggcta atgagatgta
     8041 tcactatcgc cgggcaacca ttgtggcgaa taacgacctg cgccggggag atttgctatt
     8101 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
     8161 gtttattgag tcaccgcgca ccggggaaac cattcgggta agtcggttag ctgaaccttt
     8221 ctggcaggat cactttttag gcgcgcggcg gatattgacg gaagagacga ttttgtaaat
     8281 gggtaatatt gacgaactgg caaaaggtcg gatgccacgc ggcatccgac ctgtttacat
     8341 ctgggctgaa aactcacgtt ctaagacatc aacaatgagc tggttttttg aaatggaatg
     8401 ttgtctggcg acgatggtca gccgcgaatc aatgtgatga ggaacacgaa gcgtcaggcg
     8461 tttttgctct tcttcttcac gaaaaggggc aataccgtcc tcttcacaat ccgccatcca
     8521 ctcatccagc gcgctttcgc cttcacgata taatgcctca atacttgtgg ctacaaagtc
     8581 acaataaccg tttacattga ggaacttgcc gcgaaatgcg ttgatatcgg cttcaaatgt
     8641 cactgcggct ggttgacctt taattgtgat aatgttcgat gctttattca taatctaccc
     8701 ctacacagtt aacaaaccac tccttaagac ctgctactgc gcctttatcc atcacattct
     8761 ttggatgtgg ctcatgtgtg ctgaatttgg tcgttccaat ctgaaatttt cgtctggagc
     8821 cgttgttatt ttttattgtt ccacccacag ctttaatgag tgcaacaaca tcatcccatt
     8881 taattccaga ttttggcggc tccgtcatta catcgtcgaa tgtttttcta tgacgagaac
     8941 ctagcgcgct gaccttaatc atactatcaa tccatgacgt cattgtatgg tgtcatcgta
     9001 gtctctttgc agtgtgacgt caattaatgg tgtcaggtgc tgcaaagcat ggaaaagaat
     9061 cccccatcaa ctcaacgccc ccataatctt caactccagc tcatccggca cttcgttata
     9121 cgacagcaca tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag
     9181 ctgcggtggc accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg
     9241 tggcatcgtg ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat
     9301 cactttaccg ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt
     9361 attcagggta tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg
     9421 cagcgccagg cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc
     9481 gaccagcacg gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc
     9541 ccggtacact ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct tcgccaggcg
     9601 cggtgccatc gacgccaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc
     9661 aggaatatag ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat
     9721 cacctgatac cccatattca gcgccttcgc tttctgcgca ggctggatcc aggtcaccgg
     9781 catcccgtac gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga
     9841 gggcagcgcc atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat
     9901 gaaaatagcg tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag
     9961 cagcacgccg ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg
    10021 gttgccctgg gctttgtcca ccagcgccac cagcttgtag ccgaggctta agctgatggg
    10081 ctcgatcagc ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg
    10141 ggtcagcgtt tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg
    10201 ccagccggta aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg
    10261 cactaccgcc agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag
    10321 caactggtta cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgacgataat
    10381 cgccgccgcg gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt
    10441 catcagcaca tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat
    10501 acagacgccg ccgatcaggt tgatcgccag aatcatcata ccggcgatag catccccgcg
    10561 cacaaatttc gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt
    10621 acgtcgggtt tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc
    10681 catctgctta ccgggcatcg cgtccagggt aaagcgggca gaaacctcgg agatacgctc
    10741 ggcccctttg gtgacgacaa taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga
    10801 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt
    10861 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac
    10921 ggagaaaaaa gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac
    10981 cgagcttggc gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc
    11041 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
    11101 tcgcaatatg ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt
    11161 ggagctcgcg ccagttttcg actcgctcga taacaaaatt attattaccg ggcataccga
    11221 tgcgatggct tacaaaaaca atatctacaa caactggaac ctttcgggtg accgcgcgct
    11281 ttcggctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
    11341 cgcaatggcg gaccagatgc tgctggattc caaaaatccg aaaagcgcgg gcaaccggcg
    11401 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
    11461 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
    11521 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaaccctgaa atcactgtat
    11581 actttaccag tgttgagagg tgagtaatgc gtaaaatcat tcatgtggat atggactgct
    11641 ttttcgcagc ggtggagatg cgcgacaatc ccgccctgcg cgatatccct attgctattg
    11701 gcggcagccg cgaacgtcgg ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg
    11761 gcgtacgtag cgctatgccg acagggatgg cgctcaaatt atgcccgcat ctcaccttgc
    11821 ttccggggcg ctttgacgcc tacaaagaag cctcaaatca tatccgtgaa atcttctcgc
    11881 gctacacctc gcgtattgaa ccgttgtcac tggatgaggc ttatctcgac gtcaccgata
    11941 gcgtccattg ccacggttct gcgaccctca tcgcccagga aatccgccag acgattttca
    12001 gcgagctgca actgacagcg tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg
    12061 cctccgacat gaataaaccc aacggtcagt ttgtgattac gccggcagaa gttccggcat
    12121 ttttacaaac cttaccgctg gcaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac
    12181 tggaagcgat ggggctgcgg acctgcggtg atgtacaaaa gtgtgatctg gtgatgctgc
    12241 ttaaacgctt tggcaaattt ggccgcattt tgtgggagcg tagtcagggg attgacgagc
    12301 gcgacgttaa cagcgaacgg ttgcgaaaat ccgtcggcgt ggaacgcacg atggcggaag
    12361 atatccacca ctggtctgaa tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac
    12421 gccgtctggc aaaggtaaaa cctgatttac tgattgcccg ccagggggtg aaattaaagt
    12481 tcgacgattt tcagcaaacc actcaggagc acgtctggcc gcggctgaat aaagctgact
    12541 taatcgccac cgcgcgtaaa acctgggatg aacgccgcgg cgggcgcggt gtgcgactgg
    12601 tggggctgca tgtgacgttg cttgacccgc aaatggaaag acaactggtg ctgggattat
    12661 gaataacata caaataagag actatcagcc tggcgatttt cagcaactat gcgctatttt
    12721 cattagagcg gttacgatga ctgccagtca gcattattca ccacaacaaa ttgccgcctg
    12781 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
    12841 agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt
    12901 atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa aacctttgat
    12961 taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg
    13021 ttatggtttt cagacagtta agcagcaacg cgttgaatgt cggggagagt ggtttattaa
    13081 tttttatatg cgttataaac agcaacatta aatccagctt gcaatgaaaa taacgcaagc
    13141 ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct
    13201 ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc
    13261 gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc aacacatgca
    13321 acttgaagta tgacgagtct atttacggaa tggtatatgg ggaattatat tcgtccctta
    13381 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
    13441 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
    13501 catcccggac gcggctatcc gattggcgca gcgttcttct ctgttggtcg tttttatccg
    13561 gcactggtcg gcaatgatat cggctgcggt atggcgctat ggcaaacaga tattctcgct
    13621 cgcaaataca acgccgataa gtttgaaaag cgattatcag atctggatga cgttgctgaa
    13681 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
    13741 cttggctcta tcggtggcgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
    13801 aacgctgaac tgtttgcact ggcaggtctg gatgcacaac atctgcaact gttggttcat
    13861 agcggctcgc ggggggtggg gcagtctatt ttacagcggc atattgcctc gttctcgcat
    13921 catggtttgc ctcaaggcag tgacgacgcg ctacggtata ttgcggaaca tgacgatgcg
    13981 ctggcgtttg cgtgtattaa tcgccagatg atcgctttgc gcataatgca acaagttaag
    14041 gccacgggaa gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcaaatcggc
    14101 gatcaacagg gctggttgca tcgtaaaggt gccacgccgg atgacaacgg tctggtgatt
    14161 attcccggtt cacgtggtga ttactcctgg attgtacagc ctgttgcgaa cgaaaaaacg
    14221 ctccactcgc tggcgcatgg ggcagggcgt aaatgggggc gcaccgagtg taaagggcgt
    14281 ctggcagcga aatacacggc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt
    14341 tgtcgcgata aacaactcat ctttgaagaa gcgccacaag cctataaatc ggctgaaagc
    14401 gtggtgcaat gtctggtgca ggccggttta attattcctg tcgcgcggct gcgtccggtg
    14461 ctaacgctca aaaacagtgg agggaaaaaa ggatgatctt gctacaactc tcctcagcgc
    14521 aggggccgga agaatgttgt ctcgcagtga aaaaggcgct ggacaggctt attaaagaag
    14581 ctgcccgaca agacgtcgcg gtaacggtgt tggaaacaga aacgggccgc tactctgaca
    14641 cgctgcgttc ggcgctgatt tctctggatg gcgataacgc atgggcatta agcgaaagct
    14701 ggtgtggcgc tattcagtgg atttgtccga gtccgtatcg gcctcatcat gggcgcaaaa
    14761 actggtttct gggcattggg cgttttaccg ctgatgagca ggaacaatcg gatgcaatcc
    14821 gttatgagac gttgcgttcg tcggggccgg gcggtcaaca tgtcaataaa accgactcgg
    14881 cggtacgcgc cacgcatttg gcatccggta ttagcgtgaa ggttcagtca gagcgtagtc
    14941 agcatgctaa caagcggctg gcacgattgc tgattgcctg gaagctggag cagcagcaac
    15001 aggaaaatag cgcggtgctg aaatcgcagc ggcgaatgtt ccatcaccag attgaacgtg
    15061 gcaacccgag acggacattt acagggatgg cttttatcga aggataatga aggatgaaac
    15121 tgccggaagg cgattaaacg ccatccggca gcaaatcaaa taattacttc gccggaattt
    15181 ctttcagcag ttcagtcagc agcgtccagt aatgacctac gctttcgatg tgaacttgct
    15241 catccggaga gtgtggaccg gtgatagttg gcccgataga aaccatgtcc atttccggat
    15301 acggtttttt gaacagacca cattccaggc ccgcgtggat aatctggatg ttcggtgttt
    15361 tgttgaacag acgctgatag gtttcacgta ccagatgcat caccggagaa ttagcgtccg
    15421 gctgccagcc aggatatgcg cctttcgctt cggttttcgc gccagccagt ttacccagcg
    15481 aatccagcat gctcacgacg tagtctttac cgctgtcgat cagtgaacgg atcagacagt
    15541 gaatttctac gttattatca gtcatggtca ccacaccgac gttcagggag gtttcgacca
    15601 caccttttgc cacgtctgag ttgcggatca caccgttcgg ggtggcgttc agcagacgaa
    15661 taaaggtatc gcgagatttc gcaatcaggg cagctttatc gttcgctaca gagtccagca
    15721 acaaggccag atttttctct ttctctgcca gctcgttttt caggatctcc tgataggtat
    15781 tcatcagaga tttcaggacg tcgactttat cagctgcgac agcaatggtc gcaaaggctt
    15841 cacgcgggat ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc agatccagtt
    15901 cttctgcatg acccgccagg aagcgcacca gcagtttgtt ggcattaccc aggccaacgt
    15961 ggatttcccc gccggagtga ccccctttca gaccttttaa ggttaacttg aaggtttcaa
    16021 aaccagctgg aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg ataccccccg
    16081 cacaacccat gtagatttca ccttcttctt cggagtcggt gttaatcaga atatcagcct
    16141 gcaaccagtt gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca
    16201 gcagcacttc cagcgggccg tgaaccacgt tttcgtcagc cagaactgcc agtgcagagg
    16261 ccataccaat gccgttatcc gcacccagcg tggtgccgcg cgctttaacc cattcgccat
    16321 caatataagg ctggatagga tctttcgtga agtcatgcac ggtgtcgtta tttttctgtg
    16381 gcaccatatc gaggtgggcc tgtaagacga ccggtttacg attttccata cctgcggtag
    16441 caggtttacg aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt
    16501 ttgcccaacc aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgaggaatag
    16561 aacagatttt ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca
    16621 cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa
    16681 aatgttgtca acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
    16741 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
    16801 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
    16861 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
    16921 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
    16981 gccgtaagcc gtggcggtct ggtaccgggt gcgttactgg cgcgtgaact gggtattcgt
    17041 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
    17101 ctgaaacgtg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
    17161 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
    17221 gcaaaaccgg ccggtcgccc gctggttgat gattatgttg ttgatatccc gcaagatacc
    17281 tggatcgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
    17341 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
    17401 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
    17461 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
    17521 cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat
    17581 gattaaccgt ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca
    17641 ggcacgtatt gtgatgagcg atgctgaacg taccgacgat gatttatacg atactgtgat
    17701 tggctaccgt ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca
    17761 aaaagcctgt gcggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac
    17821 gttgtacaac attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca
    17881 ggctttatca aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca
    17941 gatggagttt accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg
    18001 cgatggcccg ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta
    18061 ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat
    18121 gccgtcggtg ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca
    18181 gcacgtctta aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt
    18241 tggtttccgt ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct
    18301 gaaagcggtt gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca
    18361 gcaacaggtg ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc
    18421 ttccgatgac gcgttgcgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct
    18481 gggacgtcgc tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc
    18541 ggaagaggac tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc
    18601 atttaacccg gtgtatcgga attttgacaa aggtcttcag gaaatcactg gctggatcga
    18661 aaaacgcttg tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca
    18721 acaggagata gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt
    18781 taccgcacta ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga
    18841 ttgtctggct gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg
    18901 gtggatggag ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt
    18961 cgataaagca ggcgactgga agagcgttcc ggtaaaagac actgaagtgg ttgaacgact
    19021 ggagcacacc ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct
    19081 gaagctggaa ccggcggatg attttcgtga cgagccggtg aagttaacgg cgtgagtgaa
    19141 atgtgccgga tgcatcacat ccggcaataa tcattaaaac tgataggtca tgccaaccgc
    19201 gacaatatca tcattattaa tattcaattt gttatcgcta tccagttggt tgattttata
    19261 atcaacaaac gctgacatat ttttgttgaa ataatatgta gccccgacat cgatataatt
    19321 gaccagatct tcatcaccga taccttcaat atctttccct ttcgataaga cataacccag
    19381 cgatggacgc agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt
    19441 attggcaaag ccgccagtta ttggcgtcat tttgcgtgtt tcagaataaa aagttgccag
    19501 ataaatatta ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgccagt
    19561 gccacggctt tgcaggttct gctcgttggt gcgatctgag ttggtatagg caccactaat
    19621 ggcgaaatcg ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg
    19681 ctttttaacg tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc
    19741 gataacgccg aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat
    19801 aaagttgtcg gtctgcgccg aggagtcgcc accaaattcc gggaacatat cggtccaggc
    19861 ttccacgtca tacaacgcgc ccaggttacg gccatagtcg aaagaaccca aatctttata
    19921 cttcaatccg gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt
    19981 tccggcaaac tccgcttccc aacggccata gccagtcagt tgatcgttaa tttgtgtttc
    20041 gcctttaaaa ccaaaacgga tataactctg gtcgccatct ttactgtcgt tatcactcat
    20101 ataatgcatg gctttaactt tgccatagac atccagttta ttaccgtctt tgttatatat
    20161 ttctgcggcc tgtacggatg cagatgccac aatgcccatc accactaatg ccagagtgct
    20221 ctttttcatt ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt
    20281 tgaaaacagg aaagttttta acctgagatt gttaaagata tattacagat taataatatt
    20341 cttaaaatgt ggtaatttat taaatctgta ataaaagcgt aaacaactgc cgctaggctt
    20401 gctgatcccg cgcaacaaaa cgccatgctt tgcttgcaga tggttggcaa ccgacgacag
    20461 tcctgctaaa acgttcgttt gatatcattt ttcctaaaat tgaatggcag agaatcatga
    20521 gtgacagcca gacgctggtg gtaaaactcg gcaccagtgt gctaacaggc ggatcgcgcc
    20581 gtctgaaccg tgcccatatc gttgaacttg ttcgccagtg cgcgcagtta catgccgccg
    20641 ggcatcggat tgttattgtg acgtcgggcg cgatcgccgc cggacgtgag cacctggggt
    20701 acccggaact gccagcgact atcgcctcga aacaactgct ggcggcggta gggcagagtc
    20761 gactgattca actgtgggaa cagctgtttt cgatttatgg cattcacgtc gggcagatgc
    20821 tgctgacccg tgctgatatg gaagaccgtg aacgcttcct gaacgcccgc gacaccctgc
    20881 gtgcgttgct cgataacaat atcgttccgg taatcaatga gaacgatgct gtcgctacgg
    20941 cagagattaa ggtcggcgat aacgataacc tttctgcgct ggcggcgatt ctggcgggtg
    21001 ccgataaact gttgctgctg accgatcaaa aaggtttgta taccgctgat ccgcgcagca
    21061 atccgcaggc agaactgatt aaagatgttt acggcattga tgacgcactg cgcgcgattg
    21121 ccggtgacag cgtttcaggc ctcggaactg gcggcatgag taccaaattg caggccgctg
    21181 acgtggcttg ccgtgcgggt atcgacacca ttattgccgc gggcagcaag ccgggcgtta
    21241 ttggtgatgt gatggaaggc atttccgtcg gtacgctgtt ccatgcccag gcgactccgc
    21301 ttgaaaaccg taaacgctgg attttcggtg cgccgccggc gggtgaaatc acggtagatg
    21361 aaggggcaac tgccgccatt cttgaacgcg gcagctccct gttgccgaaa ggcattaaaa
    21421 gcgtgactgg caatttctcg cgtggtgaag tcatccgcat ttgcaacctc gaaggtcgcg
    21481 atatcgccca cggcgtcagt cgttacaaca gtgatgcatt acgccgtatt gccgggcacc
    21541 actcgcaaga aattgatgca atactgggat atgaatacgg cccggttgcc gttcaccgtg
    21601 atgacatgat tacccgttaa ggagcaggct gatgctggaa caaatgggca ttgccgcgaa
    21661 gcaagcctcg tataaattag cgcaactctc cagccgcgaa aaaaatcgcg tgctggaaaa
    21721 aatcgccgat gaactggaag cacaaagcga aatcatcctc aacgctaacg cccaggatgt
    21781 tgctgacgcg cgtgccaatg gccttggcga agcgatgctt gaccgtctgg cactgacgcc
    21841 cgcacggctg aaaggcattg ccgatgatgt gcgccaggtg tgtaacctcg ccgatccggt
    21901 ggggcaggta atcgatggca gcgtactgga cagcggcctg cgtcttgagc gtcgtcgcgt
    21961 accgctgggg gttattggcg tgatttatga agcgcgcccg aacgtgacgg ttgatgtcgc
    22021 ttcgctgtgc ctgaaaaccg gtaatgcggt gatcctgcgc ggtggcaaag aaacgtgtcg
    22081 cactaacgct gcaacggtgg cggtgattca ggacgccctg aaatcctgcg gcttaccggc
    22141 gggtgccgtg caggcgattg ataatcctga ccgtgcgctg gtcagtgaaa tgctgcgtat
    22201 ggataaatac atcgacatgc tgatcccgcg tggtggcgct ggtttgcata aactgtgccg
    22261 tgaacagtcg acaatcccgg tgatcacagg tggtataggc gtatgccata tttacgttga
    22321 tgaaagtgta gagatcgctg aagcattaaa agtgatcgtc aacgcgaaaa ctcagcgtcc
    22381 gagcacatgt aatacggttg aaacgttgct ggtgaataaa aacatcgccg atagcttcct
    22441 gcccgcatta agcaaacaaa tggcggaaag cggcgtgaca ttacacgcag atgcagctgc
    22501 actggcgcag ttgcaggcag gccctgcgaa ggtggttgct gttaaagccg aagagtatga
    22561 cgatgagttt ctgtcattag atttgaacgt caaaatcgtc agcgatcttg acgatgccat
    22621 cgcccatatt cgtgaacacg gcacacaaca ctccgatgcg atcctgaccc gcgatatgcg
    22681 caacgcccag cgttttgtta acgaagtgga ttcgtccgct gtttacgtta acgcctctac
    22741 gcgttttacc gacggcggcc agtttggtct gggtgcggaa gtggcggtaa gcacacaaaa
    22801 actccacgcg cgtggcccaa tggggctgga agcactgacc acttacaagt ggatcggcat
    22861 tggtgattac accattcgtg cgtaaataaa accgggtgat gcaaaagtag ccatttgatt
    22921 cacaaggcca ttgacgcatc gcccggttag ttttaacctt gtccaccgtg attcacgttc
    22981 gtgaacatgt cctttcaggg ccgatatagc tcagttggta gagcagcgca ttcgtaatgc
    23041 gaaggtcgta ggttcgactc ctattatcgg caccatcaag aaaatcaata acttagctta
    23101 acttctctaa gaaattgtat cttctgttat cacttacaaa cgcctatacg ttgttatggg
    23161 ctttggtagt gtaacacgta tgggcgagta gttcccaagc tcaacctcgt aacaagttcc
    23221 tttgaagctg gaagacgtgc caccgtctct aaaccttcta ccaaaactct tagcaagatg
    23281 ttccacacct ggcgcggtgt ccaatgatgc tacacgtcgt gagacgttgc agaggataaa
    23341 caggctaaat gatgggaaga gttttaagtg caatagtgga ttaaagccca cctcttcaaa
    23401 acctaacttg atcgggaaag aatcaagctc tagcacggtt tcgtccaccg gagtacaacg
    23461 ggtgcctgat aagagaagaa ctgctaagac ttcatagctt ttgtgaaaaa gcccttttta
    23521 gtggatacgc ctacggcgct tattttttgg tataaccatc aaaggcgctt tatggcttct
    23581 attgtcttga aaatgtatct ttacttgtat tgtgttttat gcgtagtctc cctgtgtagt
    23641 atgtaacttg ttaattttca tatgaataca gaggcaacac gatgaaaaac attgcagcta
    23701 taaaacgtaa taaccgcaag attcacgctc gtaagttcct gtctacgcca gaaggaaaag
    23761 cctggttaga gcgtaaacag agagagaacg aagaaagaaa actccttagt gagttgaaat
    23821 ggcttagaga ggatttctga aaatctccag aacgcaacag gacgcattct aacgctgttt
    23881 tcattgcttg atatagggat gtataggtgg tgtgtgttta atgcgtctgt gagcttgttt
    23941 tgtgccttat tttgcatttt cataatgcag aagagaacac ggaaagcctt aaaattaatt
    24001 atttgggtat ttaaggctat tttcaggtta tttaagggta ttaaacttga tttaattggt
    24061 tgaaatataa acagatagtt ctgtatctta ttgatttata aggttatttt taaataaagg
    24121 taaataaaaa ataaatagcc ataaatagct ataaatacat ctaaatacct ataaatccct
    24181 ttgaatacat atgaagccct tcccttatgg ctagattgcg agaggtgctt aacgcgattt
    24241 tcagcgcccc ccgatctgga aaatccagaa tccccaaata tcaaggaggc attacgcctc
    24301 ctcttcttcc ttcttaattc gctgtaccgt cgaaggagca acattaaaga tctcggcggt
    24361 tttacgcact gacaggccat cagaaagggc tttctttata ccttctctat ctactgcttt
    24421 tccattacca cgctttgtag gattgacggc ttgcgcttct cgtttacgtt ctaacatcag
    24481 ttcacgttca cacgttgcca ctgccgacaa catagtaagc atcatttttc ccatcgcgct
    24541 tgtaatgtca gcaatgatcc cttctttgtg aaatcgaaca attacaccac gattattaag
    24601 ctcttccact aaagcaagaa tgtctaaggt gttacgccct aaacggtcga gagagtgcac
    24661 gtgtaaggtg tcgccttctc tcagatagga taaacacgcc tgcaatcctg gacggttagt
    24721 gtctttacct gacagcttat cttcaaagac tttatcaagc gttactcctg caagctggcg
    24781 atcagtgttc tgatctacag tgctcacacg cttataaccg atattgctca taaaatcctc
    24841 ttaatgtatc gatttggcac tacttcaatg caaatgagta tatcgtattc atttggtctg
    24901 tttcaagcct tataggtacg gaatattaca cgatttagct gtattcattt ggtatgccta
    24961 atggatacac aggggaatag attaaatggt gttctgttta tacaggttta tttgttagat
    25021 caacaatttg gcatttctaa acagatgttt gaacgttgcc aaatgtcttg acaacacaag
    25081 aaaagggctt gctatgccac gcattacacg aaataaggct gtaaaaacaa aataaatttt
    25141 aaaatttgca caaacgagaa aactcattcg acaaataata gattaatttt tagccaacaa
    25201 tttctgatgg tgctctgtaa gccgcattac acaaggcttt gcgaaagtga tgtaaaagtg
    25261 aagaaagtta acattgctct tttaagctac tttttcattg aatttttagt atggaattac
    25321 acaggaataa gttagggata ttctatgatt tacacaggga atataggggg attatggcac
    25381 acacatagaa aggcattaca cagagtgagt gctatgtgac aaatggatgt aacgagaggt
    25441 tacattcagc tatctgaaat gctctttgta agaaagttgg tagtaacggt aggtcatagt
    25501 gagctagggg gtaggtcact gtgaactaca ataagactat taataaaact atattagatt
    25561 attaattaga tcataataat aatattcttt atatctatat atatatgatc ttttgccttc
    25621 cggcgttagc ttcactctct ggcaagcaac cttactccgc tacctttcgg gcttcgtggc
    25681 tggcccgttc cgctcacacc gaaagcaaag cgttactttc ttcgtgtaac tatcgttggc
    25741 ggtaacgccc acgaagaaat actattgcgc tgtgcgcagt aagagtaatc acctaaccaa
    25801 tcatactgat tgcttacgtg ttaactctct ctaactctta attaccctcc tacatttaat
    25861 aactctgtta ttctctgtag ttgtgtaatt actcgaagag agagcggcta aagctcaatt
    25921 acactctgct ttcctcgtgt aatttcacta agcctcaata caatttcact tcctacattc
    25981 tgtagtcgtg taattgtttt cggtggaaaa gccagaggcg acaccgaaag agaattctga
    26041 tcttattaat cttatctatc ttagttaatc ttcttactta tcttacttaa tctttatcta
    26101 ctttattatt aataatagct ttataagctc tctgtagctc tctgacgcaa gaaacagcat
    26161 tcaggaaggg aatcacctta gaaaagtgtt ctttgcgtca taatgtgttt tatgaaggat
    26221 tcagaggtgt ttcataacag gaatgtatag aggatacgtt aggtaattaa aaataagggg
    26281 tctaaatgct tctctaacag cattaagcag ggtagggaat ggaaacgcat tgcctacata
    26341 tataatctca ctacagagct ttacagagcg ttttataaag tgactctcta tcttatatct
    26401 accatcacct ttttatatac ctaacaaata caaaataagc cccttaaata agcctctggc
    26461 gagctaaaca gccattacat aagggaatgt attgctttta tcaaaacttc gttagaacgc
    26521 gttttagagc gttttagaca ataatgaaga atagcactac acagaagagc acgaattgct
    26581 aagaaaaaat gtaagtgcat gaaaaagatc agattttttg tgaaaaggta ttgacaaatg
    26641 tttctttata tgtaataata ggtattagag ggacagaaaa acattcttct ctaaataatc
    26701 aaaatcaatg cactttgaag ccgtcaggct tgcaaggtgt ttttctttta actgaataag
    26761 gagtttttat gaaatggttt acaccgaaac acgtcgtcga ggcatttaaa aaaggtgagc
    26821 taacacgcca ccaggtagta atgaaccgta atatggcaag gagtcgaggt tatccagagc
    26881 gagcagcttg cttcaatgaa gcactcaaga ttattgatga actaagaaaa aatgaaaaag
    26941 aatctgaaac agagtaaaga atagaaggag aaaactatga gcgaaactac agaaaagaaa
    27001 agacaaccac gcacttgctt tcatattcct gatgaagtat ttcaggtgtt gtttaaagaa
    27061 gccagaagta gacgtatcac tgttacagat tacattcttg atatgttgaa aagagaggct
    27121 gaaacactct caaataaaag caagtgattt ttatcaccat agtagcgccg tagagcgtga
    27181 ttacatcaag gagattttta atgattaacc aactaacttt tacaaaacac tatgacacgt
    27241 ttgataatgt atcaaagatt tattctgata aattccctca aggaaaagat ttagatctat
    27301 tacatattgt gctttatttt cgattcctta gttatcagga aaacaacctt aattgttatg
    27361 aaagtcacga aacattagct aagattttta agtcttcagc gtcaacaatt aaacgtaaga
    27421 taaacgatct taaagagatg ggattattag aaacatcccc acatcctgat ccgtatattt
    27481 catctttgat ttacaatgct ctccctctca cagatgcaca tattactccc ccaggagaat
    27541 catcactctc tgatctttct gaggcagcag aagcacagga acaacctaaa ggccataaac
    27601 agccttcttt tgatgttctt gatgattggg atgcgccttt gccgtgggag acggaagaaa
    27661 cacctgtttc atcaagtgaa aaggtagcta atgataatga ggaggatgtg ttagagaatt
    27721 ttgcctctct tatctgccag cagaaacaca ccagaacagg aggggcatca tttatagaat
    27781 ttgcaaattc actagcttat agacacgggc taaaacgacc aaatggtatt gaggcatatt
    27841 tttctaagaa ttacccaaat gtgtatgatg actttgatat tccattttaa taaggagggt
    27901 tatgattcaa tatttagtaa aaaatcaagt agacagaatt cagtgtaatg acacaggaaa
    27961 acgcatctac gaaacacttg cttacctcta taaaggaaag ccaacaccgc taaaatatag
    28021 cgatgtgtta catcgagcag ggtgctctga agatggttta aaattctggc tgaaacagct
    28081 atcaaatttc ggtgttatag agattaaaga attatctttc tctacattca atctcaaaag
    28141 actggataaa gaaatagtat tcatctattc cactctctaa aatctctcta tgtaattaaa
    28201 atataaaagg aaaacattat tatgatagta gctatgtatt ggttattgtt ctttctcacg
    28261 ttagaaacat atttcggggt gttttccttt aaaagaaaag gaaaagtaaa taatggcaaa
    28321 aagaaaaaac aacgttgtta agaaaatcgg tgattcagct tctcttctaa gtaaacctaa
    28381 atctattcta agacgtaaag actttaaaga gctagttacg ctctccaagc aaaatagcgc
    28441 ccctggcgaa tggaaaacag agatcataga acattctccc tctgtgcctt gtggagatga
    28501 atttaacgcc ttacaagaaa tcttatcttc aacacctggc gtattctgga aacctagaaa
    28561 aagaaaagag tatattgttg atagctctga tttacgcaaa taccagattt taggatttga
    28621 agactataac cactacgttg gttatctcgc caccaatggc ctaaataatt tagttcctga
    28681 attccattta gataatgatg atcac
//
LOCUS       DAHBHC010000066        28655 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHBHC010000066 DAHBHC010000000
VERSION     DAHBHC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.066"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            118..1659
                     /gene="tyrR"
                     /locus_tag="M2A35_003423"
     CDS             118..1659
                     /gene="tyrR"
                     /locus_tag="M2A35_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCB7199674.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(1703..2209)
                     /gene="tpx"
                     /locus_tag="M2A35_003424"
     CDS             complement(1703..2209)
                     /gene="tpx"
                     /locus_tag="M2A35_003424"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCB7199675.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            2328..3293
                     /gene="ycjG"
                     /locus_tag="M2A35_003425"
     CDS             2328..3293
                     /gene="ycjG"
                     /locus_tag="M2A35_003425"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCB7199676.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(3268..3996)
                     /gene="mpaA"
                     /locus_tag="M2A35_003426"
     CDS             complement(3268..3996)
                     /gene="mpaA"
                     /locus_tag="M2A35_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCB7199677.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4305..4943)
                     /locus_tag="M2A35_003427"
     CDS             complement(4305..4943)
                     /locus_tag="M2A35_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCB7199678.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDTQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            complement(4961..5893)
                     /locus_tag="M2A35_003428"
     CDS             complement(4961..5893)
                     /locus_tag="M2A35_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7199679.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGVTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(5893..6813)
                     /locus_tag="M2A35_003429"
     CDS             complement(5893..6813)
                     /locus_tag="M2A35_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7199680.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            6951..7850
                     /gene="pgrR"
                     /locus_tag="M2A35_003430"
     CDS             6951..7850
                     /gene="pgrR"
                     /locus_tag="M2A35_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCB7199681.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            8187..9800
                     /gene="mppA"
                     /locus_tag="M2A35_003431"
     CDS             8187..9800
                     /gene="mppA"
                     /locus_tag="M2A35_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCB7199682.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(9851..10882)
                     /gene="ynaI"
                     /locus_tag="M2A35_003432"
     CDS             complement(9851..10882)
                     /gene="ynaI"
                     /locus_tag="M2A35_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCB7199683.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            11126..11383
                     /locus_tag="M2A35_003433"
     CDS             11126..11383
                     /locus_tag="M2A35_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCB7199684.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(11433..12383)
                     /gene="uspE"
                     /locus_tag="M2A35_003434"
     CDS             complement(11433..12383)
                     /gene="uspE"
                     /locus_tag="M2A35_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCB7199685.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(12535..13287)
                     /gene="fnr"
                     /locus_tag="M2A35_003435"
     CDS             complement(12535..13287)
                     /gene="fnr"
                     /locus_tag="M2A35_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCB7199686.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(13482..13997)
                     /gene="ogt"
                     /locus_tag="M2A35_003436"
     CDS             complement(13482..13997)
                     /gene="ogt"
                     /locus_tag="M2A35_003436"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCB7199687.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(14008..15534)
                     /gene="abgT"
                     /locus_tag="M2A35_003437"
     CDS             complement(14008..15534)
                     /gene="abgT"
                     /locus_tag="M2A35_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HCB7199688.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(15568..17013)
                     /gene="abgB"
                     /locus_tag="M2A35_003438"
     CDS             complement(15568..17013)
                     /gene="abgB"
                     /locus_tag="M2A35_003438"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCB7199689.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(17013..18323)
                     /gene="abgA"
                     /locus_tag="M2A35_003439"
     CDS             complement(17013..18323)
                     /gene="abgA"
                     /locus_tag="M2A35_003439"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCB7199690.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGM
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            18499..19407
                     /locus_tag="M2A35_003440"
     CDS             18499..19407
                     /locus_tag="M2A35_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199691.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            19737..20300
                     /gene="smrA"
                     /locus_tag="M2A35_003441"
     CDS             19737..20300
                     /gene="smrA"
                     /locus_tag="M2A35_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCB7199692.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(20321..21553)
                     /gene="dgcM"
                     /locus_tag="M2A35_003442"
     CDS             complement(20321..21553)
                     /gene="dgcM"
                     /locus_tag="M2A35_003442"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCB7199693.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            21808..22791
                     /gene="zntB"
                     /locus_tag="M2A35_003443"
     CDS             21808..22791
                     /gene="zntB"
                     /locus_tag="M2A35_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCB7199694.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            23066..23239
                     /locus_tag="M2A35_003444"
     CDS             23066..23239
                     /locus_tag="M2A35_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199695.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            23269..24642
                     /gene="dbpA"
                     /locus_tag="M2A35_003445"
     CDS             23269..24642
                     /gene="dbpA"
                     /locus_tag="M2A35_003445"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCB7199696.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(24771..25706)
                     /gene="ttcA"
                     /locus_tag="M2A35_003446"
     CDS             complement(24771..25706)
                     /gene="ttcA"
                     /locus_tag="M2A35_003446"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCB7199697.1"
                     /translation="MQENQKNTKKEIYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(25758..26993)
                     /locus_tag="M2A35_003447"
     CDS             complement(25758..26993)
                     /locus_tag="M2A35_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7199698.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LRPAIEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            complement(26995..27210)
                     /gene="xisR"
                     /locus_tag="M2A35_003448"
     CDS             complement(26995..27210)
                     /gene="xisR"
                     /locus_tag="M2A35_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HCB7199699.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            complement(27310..27498)
                     /locus_tag="M2A35_003449"
     CDS             complement(27310..27498)
                     /locus_tag="M2A35_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HCB7199700.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(27491..27685)
                     /gene="ralR"
                     /locus_tag="M2A35_003450"
     CDS             complement(27491..27685)
                     /gene="ralR"
                     /locus_tag="M2A35_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HCB7199701.1"
                     /translation="MRYTKVKPCPFCGCPSVTVKDISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNRGVHV"
     gene            complement(27718..28398)
                     /locus_tag="M2A35_003451"
     CDS             complement(27718..28398)
                     /locus_tag="M2A35_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCB7199702.1"
                     /translation="MTPDIILQRTGIDVRAIVQGDDAWHKLRLGVITASDVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYESDARALFEFTSGVDVTE
                     SPIIYRDETMRTACSPDGLCNDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTQKDAWYFANYDPRMKREGLHYVMVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWK"
     gene            complement(28395..>28655)
                     /locus_tag="M2A35_003452"
     CDS             complement(28395..>28655)
                     /locus_tag="M2A35_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199703.1"
                     /translation="ERIVENTACTAEHQPERDITPVNDETMQEINALLIALGKTWDED
                     LLPLCSQILRRDIRASSELTQAEAVKALGFLKQKAAGQKVAA"
BASE COUNT         7190 a         6905 c         7013 g         7547 t
ORIGIN      
        1 acgccgaagt gcccgttttt ccgtctttgt gtcaatgatt gttgacagaa accttcctgc
       61 tatccaaata gtgtcatatc atcatattaa ttgttctttt ttcaggtgaa ggttcccatg
      121 cgtctggaag tcttttgtga agaccgactc ggtctgaccc gcgaattact cgatctactc
      181 gtgctaagag gcattgattt acgcggtatt gagattgatc ccattgggcg aatctacctc
      241 aattttgctg aactggagtt tgagagtttc agcagtctga tggccgaaat acgccgtatt
      301 gcaggtgtta ccgatgtgcg tactgtcccg tggatgcctt ccgaacgtga gcatctggcg
      361 ttgagcgcgt tactggaggc gttgcccgaa cctgtgctct ctgtcgatat gaaaagcaaa
      421 gtggatatgg cgaacccggc gagctgtcag ctttttgcgc aaaaattgga tcgcctgcgc
      481 aaccataccg ccgcacaatt gattaacggc tttaattttt tacgttggct ggaaagcgaa
      541 ccgcaagatt cccataacga gcatgtcgtt attaatgggc agaatttcct gatggagatt
      601 acgcctgttt atcttcagga tgaaaatgat caacacgtcc tgaccggtgc ggtggtgatg
      661 ttgcgatcaa cgattcgtat gggccgccag ttgcaaaatg tcgccgccca ggacgtcagc
      721 gccttcagtc aaattgtcgc cgtcagcccg aaaatgaagc atgttgtcga acaggcgcag
      781 aaactggcga tgctaagcgc gccgctgctg attacgggtg acacaggtac aggtaaagat
      841 ctctttgcct acgcctgcca tcaggcaagc cccagagcgg gcaaacctta cctggcgctg
      901 aactgtgcgt ctataccgga agatgcggtc gagagtgaac tgtttggtca tgctccggaa
      961 gggaagaaag gattctttga gcaggcgaac ggtggttcgg tgctgttgga tgaaataggg
     1021 gaaatgtcac cacggatgca ggcgaaatta ctgcgtttcc ttaatgatgg cactttccgt
     1081 cgggttggcg aagaccatga ggtgcatgtc gatgtgcggg tgatttgcgc tacgcagaag
     1141 aatctggtcg aactggtgca aaaaggcatg ttccgtgaag atctctatta tcgtctgaac
     1201 gtgttgacgc tcaatctgcc gccgctacgt gactgtccgc aggacatcat gccgttaact
     1261 gagctgttcg tcgcccgctt tgccgacgag cagggcgtgc cgcgtccgaa actggccgct
     1321 gacctgaata ctgtacttac gcgttatgcg tggccgggaa atgtgcggca gttaaagaac
     1381 gctatctatc gcgcactgac acaactggac ggttatgagc tgcgtccaca ggatattttg
     1441 ttgccggatt atgacgccgc aacggtagcc gtgggcgaag atgcgatgga aggttcgctg
     1501 gacgaaatca ccagccgttt tgaacgctcg gtattaaccc agctttatcg caattatccc
     1561 agcacgcgca aactggcaaa acgtctcggc gtttcacata ccgcgattgc caataagttg
     1621 cgggaatatg gtctgagtca gaagaagaac gaagagtaag cgcgaatatg cctgatggtg
     1681 caacaccatc aggcatatta aattatgctt tcagtacagc cagagctgct tcgtaatccg
     1741 gctcggtggt gatttcatcc accagctggc tgaaaatcac attgtcattt tcgtcaataa
     1801 ccacaacggc acgcgctgcc agacctttca gtgggccatc agcaattgcc acaccgtagg
     1861 cttgcaggaa ttcagcgtta cggaaagtgg agagggtgat aacgttgttc agaccttctg
     1921 cgccgcagaa acgagactgg gcgaacggca gatcggcaga aatacacagc acaacggtgt
     1981 tgtcgatctc ggttgccagt tggttaaact tacgtactga tgcggcgcaa actccggtat
     2041 caatactcgg gaaaatgttc agcactttgc gtttacccgc aaactgaccg agggtgacgt
     2101 cagacagatc ttttgctacg agagtaaaag tctgcgcttt gctacccgcc tgcgggatgg
     2161 aattggcgac tgtaaccggg ttgccctgga aatgaacggt ttgtgacatg attatctttc
     2221 ctgtttacat atagttaacg tcacacctag tttatgctaa ctgtcaataa cacagcaaac
     2281 gctatttgcg cttaatccgc agaccaccgc gacaacaagg agtaaagatg agaaccgtta
     2341 aggtatttga ggaagcctgg cccttacata ccccgtttgt gattgcccgg ggaagtcgca
     2401 gtgaagcgcg cgtggtggtg gttgaactgg aagaagaggg tattaaaggc accggcgaat
     2461 gcacgccgta tccgcgttat ggagaaagtg atgcctcggt aatggcgcaa attatgagcg
     2521 tcgtgtcgca attagagaaa gggctgacac gggaggagtt gcaaaaaatt ctccctgccg
     2581 gcgcagcacg taatgcgctg gattgtgcat tgtgggatct ggccgcgcga aaacagcagc
     2641 aatcgctggc tgatttgatc ggcataacgc ttcccgagac agttatcact gcacagacgg
     2701 tagtcatcgg tacgcctgat cagatggcca atagtgcatc aacactctgg caggcaggcg
     2761 cgaaattact gaaagtgaag ctggataacc atcttattag tgagcggatg gtggcaattc
     2821 gcacagctgt gcccgatgcg acgctgatcg ttgatgcaaa tgaatcctgg cgtgcagaag
     2881 ggttggcggc gcgttgccag ctattggcgg atttaggcgt tgcgatgctt gaacaacccc
     2941 ttcctgcgca ggacgatgcg gcgctggaga attttattca tccgttgccg atttgtgctg
     3001 atgagagttg tcatacacgt agcaatctga aggcgctgaa agggcgctat gagatggtta
     3061 acattaagct cgataaaacc ggtggtctga cggaagcgct ggcgctggcg actgaagcgc
     3121 gtgcacaagg tttcagtctg atgctgggct gcatgttgtg tacctctcgt gccattagcg
     3181 ccgctttacc gctggtgccg caggtcagtt tcgccgatct tgacggaccg acctggctgg
     3241 cggtagatgt ggaaccggcg ctccagttca cgacgggcga attgcatctt taggatgcca
     3301 gcgcagcaag tttgccatcg caaagagata tttttcgctg gcttcgtcgg aggagatggg
     3361 cggaaactct gcggtgatgc aatgcaagtt gagatctgca caccaactgc cgaaagagcc
     3421 aggcgtttca taaccgacgc tggttaccag aggcaattca aacgcctggg ccagccattc
     3481 gcctaattcg ctgtgtctgg gatcttcaat acaggccagt ggatcgtgga atgagaccac
     3541 ccaggcaggt tgaatgcggt gtataagctg gcacagcgcc tgggtttcag gttctgagcc
     3601 aggtttgtca cctgtcagta atacgacatc acgttcttca gcggcgctgt tccagcgata
     3661 aaccgtttca ccttccttcc agttcgccgc cggaaagttt ctgtttaaat ccacaccatt
     3721 agcattggcc cgtaacccca gctggcagcc gtcaggattc acacacagca ccacatgatg
     3781 gcggcgcaaa gaaggtgtca aagtccgcag agcacaggag agcgtgacga ccgaagagtt
     3841 ttcatcaccg tgagtgccag ccagaatcaa accactttcg cgactggctg caggggcagg
     3901 aaaccagatt aacggcgcgc ccaataatga acgtccgtaa tgttctgttc cgggcggaaa
     3961 tgcgccgcgt tcggcgcgtg ggcggtttac ggtcatgatc gtctctggtt caggaagtat
     4021 tacaggcagt gttgtgcaaa ttttgcggat tatcaaatcc ttttcttcaa cgctatgtca
     4081 tcgatcttgt atcacttttt tgccggaagt tgtttgcatt tttattgatg tgcagatatt
     4141 agaattatgc tgacttagat gtaagtcgta atgttatata catggcgtat tttattctta
     4201 ctggttctac acatgttaat cgcatcggat tgatcagttc accttaataa gtttaataaa
     4261 tcgtactgca catagtacat tacgatggta actatgtaat tgatttacat gaataatggc
     4321 ttgtcaccat cagtcacggg ttgattgata cctatattat caccaatgtg tttctcaggt
     4381 ttatcgatga tatctgtgat taatgctgcg acactcttac gtgatacgat cgtacctttg
     4441 aatggttcat tgcgtgaggt aagctcataa tcaataatat cttcatcagt cagccaggca
     4501 gggcgaagaa ttgtatattc cagtccagaa gcctcaattg catccgcagc cctgcggaat
     4561 ggttttaggg gctcaccaat aactgcatta ttccattcgc caaattttcc tggaacttca
     4621 tcataaatac cgagggaaag aacaaagatc agacgtttaa catcacaggc tttcatcgca
     4681 gcgatcactg aatttgcctg agtatctaaa tcttcccccg taaggttggc atacacaacg
     4741 tcctgtccct gcatggcctg cttcagtgct gcatgattca gtacatctcc cataataatc
     4801 ttgctattag ttggataggg tttgtggatt tttgctggct gacgggcaaa taaagtttgt
     4861 ttaatggttt gtttatctgc cagttgatta atcacatggc gggctatctg accgccagca
     4921 ccaagaatca gtacattttt cataatattt ctcatgaaac ttatcccaga tgggtgttga
     4981 aaaaaggaat aactttttcc agcgcctcct gaactgcttc cggtttgtca tacagttcat
     5041 aatgtgagaa atcgggtaat gaaactaact gacgatcctg cgaaaccatc gcccggccat
     5101 agacttccag tccatcacga taagcaccga aggcaccgac tttttcacca actactacca
     5161 tcaccggctg agtcagtaac acttcagtaa atgcgaaggc atcccaggcc agcgttttct
     5221 gtgcatgtga gaacaacatt tttgtcgcac ctcccggttg ttgcccgcgt ggtgttttat
     5281 agtagtcagt tgcttcataa acatcgcgtt cggtcactcc atgggctttt gctgcatcca
     5341 ggctggcggg aagtaattcg ttgatctgta attctccacc acgagcctct ttggttcgtt
     5401 gcgacgccat tgcattcagt gcgccaatcg gatcgtaatt gctgaaacct tcccgaaaaa
     5461 gacgcccgat attaacaccc gtaatactca ctactgcttt aattcgtttt tctgtcagcg
     5521 cggcattaat ggcatagcca ccacccccac acactcccag cacgccaata cgttcagcat
     5581 taacatatgg cagagttact gcataatcga taacacggct aatatcctct acgcgctgtg
     5641 tcggatcttc tatccagcga ggctccccac cagattctcc ctggaaactg gcgtcataag
     5701 cgagaacgag atagcctttt tcagccaatg ctttgccata gatattgcca gacgtctgct
     5761 ccttacaact gccaaaagga tggacgctga taatcgccgg atactgtttt gactcatcaa
     5821 aatttggtgg aaactgaata ttggcagcaa tatgccaggc catatcacta ttgcggattt
     5881 gaatatgttt cattacagag tttcctcaaa gaacggagct aatactgaaa ttgcttcagc
     5941 cacataggct ttgccgtcat aaagatccat atggtttgcg ccttcaacaa tgtgatagcg
     6001 tttatcctgg cttgatgccc gatcgtacag gtcgtcactc atccatttgc tccctgcctg
     6061 gctgcccgcc acaatctgca tcggctgagt caggtacact tccgccatat ggtaagcatc
     6121 ataggtaata atctggttaa ggctgcgcaa agtagcgtaa cccggtgctg ttggatactg
     6181 cgcgcgaggg gtgtggtaat actcccaggc ctgacgcagt tcttcgttcg gcgcatcgga
     6241 ctccttcatt ggtgccagtg gcataacggc atattctccg ctgctaatat cgctggttcg
     6301 ggcgtttgaa cccgcttcaa cgtatggcag ggcatcaata gatttcacat tgttttccca
     6361 accattacgg aacatcgaac caatattgac cgcactaacg gtaccgatgg ccttgatgcg
     6421 gcgatcctga attgcagcat tggctgtata tcctgcaccg gcacaaattc ccatcgcacc
     6481 aattcgggta ttgtcgacat aagaaagcgt tgtcaggtaa tcaatcacgg cactgatgtc
     6541 ttcagtacga atgtatgggt tttctaactg acgcggctcg ccgccacttt caccttgata
     6601 agatgcgtca taagcaatag tgacaaatcc cttttccgcc agttttttgg cataggttcc
     6661 ggccgtttgt tctttaacgc ccccacctgg gtgagataac acaattgcct gatactgacg
     6721 ggtttcatca aattttggag ggaaatagat cactgcagac aaagagatgg ttggattatt
     6781 gctgttagtg aagctgactt tattattcat cattccgttc ctcatggagt tgtcggttcg
     6841 ttttaacggt tggtgatatc actatagata ttgatcatta agttgattag acccaaaatc
     6901 atgattagac ctatcattta aatgattaat agattgcatg gagatactga atgaagcgtg
     6961 aagaaatcgc tgatctgatg gcgtttgtcg tcgttgcaga ggagcgtagc ttcactcgtg
     7021 cagcagcccg cctgagcatg gcgcagtcag ctttaagcca gatagtgcgt cgtatagaag
     7081 aacgattggg attgcggctt ctgacgcgaa ccacgcgcag cgttgttcca actgaagcgg
     7141 gcgagcatct tttgtctgtt cttggcccta tgttgcatga catagattca gccatggcat
     7201 ccctgagcga tctgcagaac cgcccatccg ggacaatacg tattactact gtagaacatg
     7261 cagcaaaaac gatattgtta ccagcaatgc gcacattcct gaaatcgcat cctgaaattg
     7321 atattcagct caccattgat tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag
     7381 gcgtccgtct gggtggggag atggataaag atatgatcgc cattcgaatc gggccagata
     7441 taccaatggc tattgttggc tcaccggatt atttttctcg ccgaagtgtt ccaacgtcag
     7501 tgtcacaatt aatagatcat caggcaatta atttgtatct tcccacatcg ggtacagcaa
     7561 atcgctggag attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa ggtcagcttt
     7621 tactgaatac gatagacctg atcattgatg ctgcaattga tgggcatgga ttggcgtatc
     7681 taccttatga tcaggttgag cgggctatta aagaaaaaaa actgatacgt gttttggata
     7741 aattcacacc agatttaccc ggttatcacc tgtactatcc acaccgtcga catgctggct
     7801 cggcattctc attatttata gataggctga agtataaagg tgctgtttag cactacttgc
     7861 tgatacatta atttaatttc tctcttaacg tattctcagt tcctttcaac gttttggtta
     7921 ttttttattc ttcgtacaat ggcgacagat gctgattttg ataccgaaaa ctggtttgaa
     7981 cgtgcgaagc ctgaacgtag tgttcggtgt tctatgtgct ttgccgcatt ttggcgtcga
     8041 ttatttacac taaatctgat ttaatatatt gatacataaa acattttatg cttcctgtgt
     8101 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
     8161 ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagtcacg tgttgtgcgc
     8221 tgttggtcag cagcatttct ctttcgtatg ctgcagaagt tccgagcggc acagtactgg
     8281 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
     8341 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
     8401 agaacgagaa aggggagatt gtccccggcg ttgcgactca gtggaaaagt aatgacaacc
     8461 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
     8521 cgcaagattt tgtctacagc tggcaacgtc tggtggaccc gaaaacgcta tcgccatttg
     8581 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc aattattgat ggtaaagcta
     8641 cgcctgacca gcttggcgtc accgcagttg atgcccatac cttgaaaatt cagcttgata
     8701 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
     8761 ccaacgtaga aagcggtaaa gagtggacga aacccggaaa tctgatcggc aatggcgctt
     8821 atgttcttaa agaacgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
     8881 gggataacgc caaaacggta ctgcaaaaag tgaccttcct gccaattaat caggaatccg
     8941 cagccactaa gcgttacctc gcgggggata ttgatatcac cgaatccttc ccgaaaaata
     9001 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgccg cagctcggga
     9061 cctattatta tgcgtttaac acgcaaaaag ggccgacggc agatcagcgc gttcgtctgg
     9121 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtattaggg acgggcgaaa
     9181 agccagcgtg gcattttaca ccagatgtta ccgccggatt tacgccggaa ccttcgccgt
     9241 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
     9301 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
     9361 aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta gatgttaaat
     9421 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
     9481 tccgcgcctc atgggtgggg gattataatg aaccctccac tttcctgaca ttattaacgt
     9541 caacgcattc aggaaatatt tcacgcttta acaatccggc atacgacaaa gttctggccc
     9601 aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg gcagaaaaaa
     9661 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
     9721 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agccggacta
     9781 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
     9841 gatgtctgat ttagcgcccc tgttcctgcg gtgtaatgtt atccatatac agcgtctggc
     9901 tggggaaggc aaagtccgcg ccgtgtgact gcacaatatc gataatcttc aaataaacgt
     9961 cttgctgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
    10021 tattcaatga cgagtcggca aactggttga aataaaccag taaggtttgt cgctggtcga
    10081 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
    10141 catcctcata acgtaaacca atggtcgtgg taatgcggcg gttggtcatt cgtcctgggt
    10201 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caatggacga ttatcaaagg
    10261 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
    10321 gtgaacggat ccagtcgcca atactgaaag gacggtcgaa atagagcatg atcccggaaa
    10381 agaaattact cagaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
    10441 aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc ataaagcaga acaagcacca
    10501 caataatggt gattttgata atgcgcgaca taatccgcgc actggtgata tcgcgacctt
    10561 ttttaatctg ctgtttttca aactgattaa tcagcaaaaa tagcttaatc gtcagaataa
    10621 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
    10681 agttttctat aacataatta ataatgctac caacagcact gataattatg gtgtagatta
    10741 aaaattgcac cgcatggaat aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa
    10801 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
    10861 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
    10921 ggaaacccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
    10981 aaggaagtag caacattagc taactaatca atgcgttgat tgtaaatcca gctaagaggt
    11041 gaggttttca gagcagacaa cggtgaaatg tcatggtatt gttacgttta ggtaacaaga
    11101 aatttgtctg cacaaggatt acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa
    11161 gaaatttctc tttggtttgt tggcggtttt cattattgcg gcgtcggttg tgactcgcgc
    11221 gactatcggc ggcgttatag aacagtacaa tattccgctg tctgagtgga cgacatcaat
    11281 gtatgtgatt cagtcatcga tgatttttgt ttatagcctg gtctttactg tgttgctggc
    11341 aatcccgttg ggaatttatt tccttggcgg cgaagagcag taagtaaaaa ataggcccga
    11401 taactcgggc cttgtcagtt attgaagagt cgttaatcgt cttcttcgtc atccagttca
    11461 acgggtgtct gatactggtc aggtttaatg accaacagat cgcagcgaag atgatcaatc
    11521 acctgttccg ccgtgttgcc gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc
    11581 agaaccacaa tccccgcctg taagtgctcc gccaaatcag gaatcacctc ttctggcaga
    11641 cctttttcta cgtgcgtcat gttttcatta atgccgaatt tctgccgcag agctttcatt
    11701 gccagcaaat gttgcccacg aatggcatcg ttataaacgc tcggatcaaa ttccggcagt
    11761 tcaatcgcga tattaattgg cgttaccgga taagcgccaa ccagatgaac ttcggtatgg
    11821 ttgacttgtt ctgccagttc gatcgtctct ttgaccagtt tttcattgag cgcattatga
    11881 tacggctctt cactggcgag attcaccgcc accagcgcct tgcctccttc cggccacggc
    11941 tggtctttca ccatccacac cgggcttggg catttgcgta acagatgcca gtccgttggc
    12001 gtaaaaatca ccgcttccag acggtcatgt tggtgcgcca tttttagcac caaatcgtgc
    12061 ccgccgctga tcacttcctg aatgatggct tcgaagggac ggttatgcca gaccacttta
    12121 atttcaatgg gaacgccagc attgagataa tattttgcct gctcgtggat ccaggctgta
    12181 cgctggctga tgacgccctg acgcatagcg gtacgttcgt ccggggagag cagggtggtc
    12241 atttcgtatg agaagtcata gatcggcaaa aaggctttaa ttttgccacc aatccgttga
    12301 tgtaaataaa cagctcgccg caatgctggt tggtcgtcct ggttaggatc gataacaacg
    12361 agcatgttct gatacatagc catacagggt ctccttacaa caactgtcaa cgcagtttgt
    12421 aattaaaaga ttaacccata tctggtgaat gaaacagtga tgaaccttct gccagatcaa
    12481 taaatcagaa aaatttaatg atatgacaga aggatagtga gttatgcgga aaaatcaggc
    12541 aacgttacgc gtatgaccag caagctgggc cagcgcatcg ttattttcga tggtgatgta
    12601 tttacctttg actgccagca taccgctttt ctggaagcga cccagcagac ggctgatggt
    12661 ttctaccgtc aggcccagat agttaccgat atcgccacga gtcatcgtca ggcggaattc
    12721 acgaggggag aagccgcgtt gggcaaaacg acgggacagg ttgtagatga atgcagccag
    12781 acgttcctcg gcatttttct tcgacaacag caggatcatg tcctgatcgc ctttgatttc
    12841 accgctcatc agacgcatca tctgctgacg cagattcggc attttaccag acaaatcgtc
    12901 cagcgtttcg aacgggattt cacataccat cgaggtttcc agcgcctgcg cgaagctcgg
    12961 gtgatggccg ctgccgatgg cgtcaaatcc caccaggtcg cctgctaaat ggaaaccagt
    13021 gatttgctcg tcgccttgct cagtgatggt ataactttta atcgtaccgg agcggatggc
    13081 ataaagcgat ttaagttcat caccagcctt aaacagcgtc tggcctttct gaataggctt
    13141 cttccgctca atgatattat caagctgatc aagctcatgt tcgttgagtg tgaacgggat
    13201 gcaaagctgg ctgatgctgc aatcctggca atggatagca caaccgccag actgaatgcg
    13261 ccgtataatt cgcttttccg ggatcatagg tctgctcaag ccgtaattga tatttgtcaa
    13321 ttttaacatc tttttaggga gcaagtaagt ctaagcaaac cttaacagca gagaattccg
    13381 atattagatg taaatatatg tctatctatt tgaaaaccct taagttgtta agggtaactt
    13441 tacataaaag tgtgaacaag ctggcacaaa ttgtttaatg tttacagcaa aagataacct
    13501 tcgtggcgca ataaccactc ttttcgctga actccgcctg catatccggt catggtgccg
    13561 tttcggccaa taacccgatg gcaaggtacg acgatgctga tgggattcga tccgtttgcc
    13621 gcaccaacgg cacgcgccgc gccagggcgg cccaattgtt cagccagttg gccgtaatgc
    13681 attacctgcc cgcaggggat agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc
    13741 ccccctgtgg cagtgggaag cgtatcaata atgctaagat taccggcaaa ataatcacga
    13801 agcttgtcgc ttaaaccgcc tgggttggtg gcagaaatgc gctcatagcc ttctttgcga
    13861 taatggatgt ccagcagctg caccatgcgt tcgctgtact cttcccattc aaccgcccgc
    13921 aggcgaaatt gctcatcgca aatcacccac agtggaccca gtggcgtggc aattttttct
    13981 tcaagtaatc tcagcatccg ttctctctta agacaaacgt gggtaaatcc ccggacctat
    14041 cggcagacca acaagatacc acgccagcaa catcagcagc cataccacca aaaagataag
    14101 cggatagggc aagactaacg aatagtaagt acccagtttc gcgtctggtt tgtagcgttg
    14161 caggaatcca agaaacagtg gaacaaaagg agataccggc gctaaaggca atacggatga
    14221 gtcggcaata cgaaagagga tttgcgcaaa tgccgggtga aagccaagta gcataaacat
    14281 tggtacgaaa atgggggcca gaatcgacca gattgcggaa ccgctggcaa taaacatgca
    14341 taagaaagag gaaagcaacg ccagaccgac aaacgccggg atgccgctaa gccctgaact
    14401 ttcaagtata tcggtcagcc ccacggcgat gaatttcccc atgttgctcc agttaaacat
    14461 ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat cccgccatct ctttcatcgg
    14521 ttcaatcatt aaatgcggta aatccgcctg acgtcgaatt gtgcgggtag cgatgccata
    14581 agccagcgaa accacaaaga aaaaaagaat gattagtggc acgatacctt taataaaggg
    14641 tgatggcatc acggtgtgat taatcggatc gcgcaatatc ccgttttccg ggatcaccat
    14701 cagcgcgatc gcagcaataa aaagtagcga tacgacacct gctatgcgta aaccaaaacg
    14761 ctgactttcg gtcaatgtct gcagtttctc atcgctgttt ccctgccatt gacctaaccg
    14821 tggctcgatg attttgtcgg ttatcaagcc gccaacaatc gtcagtacga ctacggagct
    14881 ggccataaaa taccagttat caattacact gacgtgcatt tgcggattga acgcagccgc
    14941 cgcttccgtg ctgatccccg acagcaacac gtcggttgtg acaatcagta aattagccgt
    15001 aaagccgcaa cctacgcctg caatggcagc cagtaaacct gcaactggat gcctgcccac
    15061 cgccagaaaa atcagcgcac ccatcggtgg catgatcact aacgccgcat cggaagaaat
    15121 gtggctgaaa aaagcaataa acagcaccat ataactggcg tagcgggcat taacatgcga
    15181 tgccatttta accattagcg ctggcagtaa gccgacgcgc tccgctaggc cggcacctaa
    15241 aaccagcgcc aggatcgctc caagtggagc aaaaccgcta aagtttttaa tcacattggg
    15301 taaaaaccag tgtaatcctt ccacactgag caggtttttc accaccaccg gcgtaccatc
    15361 ggtcgggttt ttcgcactga cgccaaaggc tgacaaaatt gccgtcgtca ccatgagtac
    15421 gattatcaaa tagataaaga gcaaaaaagg atggggaacc ttgttaccaa ttctttcgac
    15481 ccagccatag agcttcccgg attgggagga cgacggtatg gatgacatac tcatgggcat
    15541 tcctcggttg ttgtgtttgc gttgttgtta ttttaaaggt gacggtgtca cgtttttcgg
    15601 gatagggcag tgatacggtt gcgtgtccgt aacttgctga tgctcttgtt ggcattcttg
    15661 caatagccct gaatcaatga agagattgac tgtggttgct gccatagttt tcgccgccag
    15721 cagcattcct ttatgagcaa tagatgttcg cccctggcta accagttgcc acgtatgtag
    15781 cggtgtaccg acggcaaaac aggggctgaa acactgggca acaggcagtt tccaactgac
    15841 gtcgccgaca tcagtcgatg ccgcaagcac gttatcggtg gcggcatatg gagcgacctc
    15901 attcgccagt accgtttcac gatgacgtag tgcaaaaacc ttgccgtttt cgccaccggt
    15961 tgcggcgata ttattcagac tgttttgccg atcgttgggg gtgagcgtag cctgaatttg
    16021 tttcgcaaaa gccagttctt cggagttcca ttccggggta ccaaaatggg atagggcatg
    16081 gtacatggca ttttctaagg tgcgattcgg gagataactg gaacaggctt tgtcgaagcg
    16141 gcattcaacc gtggtttcgg tcatcaatgc cgcaccttcg gcgattttgg cgacccgatc
    16201 ataaatatgc tgcacgtcgg tcatttcggg ggcacggata agataaagca cttctgcctg
    16261 cgcctggacc acgttgggcg agatcccgcc gctatttgtg atggcatagt gtacgcgcgc
    16321 tttttcaata atatgttcgt tgaggaagtt ggtgccagtg gtcatcaacg ttacggcatc
    16381 aagggcgctg cgtcccaaat gaggggaatt cgcggcatgt gctgcgatcc ctttaaagca
    16441 ccatgatgcc tgaatgtttg ccagcgtgcg ggtattgaac atgccggcaa aggcttccgg
    16501 gtgccaggtg agtgccgcat ccacatcatc aaataccccc tcgcggacca tgaacgtttt
    16561 acccgagccg ccttcttcgc caggacaacc ataaaagcgc actgtgccgc cttgcccata
    16621 ttgttccagc catttcttga cggctattgc agcggcaaag gcggcggttc ccagcaaatt
    16681 gtgtccgcaa ccgtgaccat tttcaccggg cgtcgcggat gtaggttgcg cgcaaccagc
    16741 ttgctgactt aaacctgcca gggcgtcata ttctcccagc agggcgataa ccggtttgcc
    16801 ttgaccaaac gaagcaataa atgcatttgg gatattgcct acgttgcggg taacggtgaa
    16861 gcttgcagat tccagcgccg aagccagatg ctccgctgac cagaactctt caaaacgtgt
    16921 ttctggatga tcccagattt gatcggcaat atcggtataa cgttggcgat cggcttcaat
    16981 cgcatcgtcg ataaaacgat agatttcctg catcagatac ctcgcgtcca gggaaaattg
    17041 agcgcggtgc gcgccagcgt ttcgacggca atagcgagaa cctgctcgtc aaaatcgaat
    17101 ttttcgttgt gatgacctgc cgccagctgt gtgccaaaca ccacgtagga ggcttgccct
    17161 tgatgttgct gcacgcgggc catcattaat gtggcatctt cggaacccgc aggcgcttca
    17221 acacgttcaa tggcctgatt gacccccgcg acctgagccg cctgactttg caaccatgcg
    17281 acccattgcg gcgaaggaga actggcggta gctgcaccca tcagacgagt ttcaacgccg
    17341 acaccataca tggttgctgc gccctgaatc gcttgttgtg cacggtcaaa aacatattga
    17401 ttaatgacgt cgctggcccc gcgtgtttcc actttcagca acgccgaggc aggaacaacg
    17461 ttacggccgc ttcctgcctg cataacgccc acgtttactc tggaagctcc ttcgctgtgc
    17521 ggggcgattg catgcagtgc aagagttgct tgtgctgccg ccaacaaggc attgtgaccg
    17581 tcttctggtt ttgcgcctgc gtgagcggcg gtcccggtga agtgcgcgtc aaatttggtg
    17641 gttgccataa aattatcact gccgcacacc acggtgcccg caggtacgcc agtgccaatg
    17701 tgcacggcag taaaataatc aacatcatct acgacacctg catcgaccat cgcccgcgcg
    17761 ccacgcgtac cttcctctgc aggctgaaaa atcagtttga tgacgccatg tagtccggac
    17821 tcgaattgtt taagggtatg cgccatccca agcccaatgg cggtatgtcc atcatgacca
    17881 caggcatgca tcattccggc gttacatgac gcaaaaccgt cgcggtaggg gcgatggctg
    17941 acatcctgct cttcactgag atccagcgcg tccatatcga cacggaaagc catcaccgga
    18001 ccggggcgac cggtatccag ggtggcgacg ataccagtga aaccaccttc aaaaaccgca
    18061 atccattgtg ctagcgcacc ctgttgacga gcgcgctcga attcgcgttg tagagtgagt
    18121 tcatcaggta gtcccatccg gctactttca ttaactactt cgcggcccag cgccagtgaa
    18181 tagccgagct gctgcaattc ttccgcaaca agggtggcag tgcggaattc cacccagcca
    18241 gactctgcat agtgatgaaa atcacgtcgc cagtgcgata attttggggc aagcgaatta
    18301 acaaattgat tcaaagactc cataaccttt cccgtcatca gtaaaaagtg tgacccggtt
    18361 catgtagcga tagtttttac ttatcactaa ctgatttttc acagttttaa ccgttcataa
    18421 attaccctga cacaatcatc tgcattaaag tagatgccag tttctttggt ctgataaata
    18481 acggttatcg gtggcgtcat ggcttttcag gtaaaaattc atcaaattcg tgcttttgtt
    18541 gaagtggctc gtcagggcag cattcgcgga gcgagccgaa tgttgaatat gtcgcaaccg
    18601 gcactgagta aatctattca ggagctggaa gaaggattag cggcgcaact cttttttcgc
    18661 cgtagtaaag gcgtgacgtt aactgatgcc ggtgaaagtt tttatcagca cgccagtctt
    18721 attcttgaag agctgcgcgc agcgcaagag gaaattcgcc aacgacaagg gcaactggca
    18781 gggcagatta atatcggcat gggggccagt atttcccgca gtctgatgcc agctgtcata
    18841 tctcgttttc atcagcagca tccgcaggta aaagtacgca ttatggaagg gcaactggtg
    18901 tcgatgatta atgaattgcg tcagggagaa ttggatttca ccatcaatac ctattatcag
    18961 ggaccgtacg accacgaatt tacttttgag aaattactgg aaaagcaatt cgcgatcttt
    19021 tgccgcccgg gacaccccgc cattggtgcc cgttcgatca aacagttact ggattacagc
    19081 tggacaatgc cgacgccaca cggcagttac tacaaacagt tgagtgaatt gcttgacgat
    19141 caggcgcaaa cgccacaggt cggtgtagtc tgcgagacgt tctcagcctg tatcagtctg
    19201 gtggcaaaaa gtgattttct cagcatactg cctgaagaaa tgggctgcga tcccttgcac
    19261 ggacaggggc tggtgatgtt gccggttagc gaaattttac cgaaagcggc ctattatttg
    19321 attcagcggc gtgatagtcg ccagacacca ctgaccgcgt cattaatcac gcaattccgg
    19381 cgagaatgcg gctatctgca aagttaaaac tgcataaaaa aatagagtct gtcgacatcc
    19441 gccagactct acagtacaca cagcagtgca tccgcgtctt aagtccggca ttgtctcctc
    19501 tgcgccggtg actgtgttat aacggtttta acctttagtt gccaattttc ttcagtgaca
    19561 gatttcacga aaatataagt tacattatta atatcgtgaa tgaataatca tgcataagca
    19621 ttttgcttaa aatatcggca atatttggaa cttattactg gaattttggg taatacgttg
    19681 ttggaccgac ccggtctggt tatcatatcg cgctcttaat tgcgggagga tgtaacatga
    19741 accttgacga caaatcgctg tttcttgacg ccatggaaga tgtccagccg ctgaaacgtg
    19801 ctaccgatgt ccactggcat ccaacgcgta accaacgtgc gccgcagcgt atcgacacgc
    19861 tgcagcttga taatttcctc accaccggat ttctcgacat catcccacta agtcagccgc
    19921 tggagtttcg gcgggaaggg ttgcaacatg gggtgctgga taagctgcgc agtggtaaat
    19981 atccgcaaca ggcgagcctg aatcttttgc gccagccggt ggaagagtgc cgcaaaatga
    20041 tgttcagttt cattcaacaa gccatggctg atggtttgcg taacgtgctg attattcatg
    20101 gcaaaggacg ggatgataaa tcgcatgcca atattgtccg cagctatgtg gcgcgctggc
    20161 tgaccgaatt tgatgatgtt caggcatatt gcactgcgct accgcatcat ggcggcagcg
    20221 gggcgtgtta cgtcgcacta cgtaaaacgg cgcaggcgaa gcaagaaaac tgggagcgcc
    20281 acgctaagcg cagtcgttga tctcgagacg catccgcggc ttatgccgcc agcacgcggt
    20341 tgcgtccatc atttttcgcc cgatacaaag catcatcaac gcgtttaaac agttcatcga
    20401 tgctttcatt tccttcgtga tgcgccacac caatgctgac ggtaaagcgt ggtaagcccg
    20461 aaatactcac ttttgccacg cttacacgga tagtttcagc cagcgaaagc gcggtatcca
    20521 gtggggttct tggtagcaat aagacaaact cttcgcctcc ccaacgaaac accaaatcgc
    20581 ctttgcgagc gcaactttca agggtgcggg cgagggcgca taacacctca tcacctttag
    20641 aatgcccata gagatcgtta atgtgtttaa aacgatcggt gtcgatgagc aacaagctgt
    20701 aatcctgagc gatggcgaga tgctgcattt ggcctggctc ggtaatgtga taaaactgtc
    20761 gccgattcag taatccggtc atcgcgtcat ggtgagcagc atgttccagc tgctcctcca
    20821 gccgtttttg ctcagtaata tcatgcacaa tacataacat gagcttgtcg ccataaattt
    20881 caatcggccc ggcataggtc tgcacatgac gagtagatcc atccgccagt ttatgaacaa
    20941 aattcaaagg tttatgaccg ccgggtaaat gcgaaatttc atgcatgata ggcatgacgc
    21001 gacgcccgag catattaatt tcccaggtat gtttctggca catcgtttca tggttataac
    21061 catagaaatt gagcgcggcg aggttagcat cgacgatttg gccatctcgt gaggggtcaa
    21121 tcaacaacat tggtgcagag ttagtcagaa aaaagcgcgc ataaaaacct tgttttttgc
    21181 gctgataatt tgccgagcga ctggctttta aacccagcgt tgccggcgct tcgatacctt
    21241 cgaaaataat caccggttct gtttctgtca gctctcgcaa aacaagccga cagcttaatg
    21301 ctgtttcctc ttctttacgc tgaacagtga ggatttcgat aatatcgtgt tggttttgca
    21361 gatcggagag gtatttcggc agttcttttt gtgaggagac ggaatagggt ccggttcgta
    21421 gctgactaaa cgtgaggtct tgcatcaaca gtcgcgccgc gctattggca taaattaact
    21481 gttcctcaaa gggcgaaaca atccagacag gactggtgag taagtccagg gtattgaagt
    21541 tgtgcgtaat cattgagatc ccgttatttt tatcaatttt tgttgctatc cgatcgcaaa
    21601 aaagccacgt catatgatca gataattctg ataatgatag acgctattta acacttcaca
    21661 cggtttgtat acggaaaagc attttgcttt ttgtattcaa tttagacaga attttattaa
    21721 tcatttcagg gtaatggggt gatgagatgt tgcgtaacag ggccagaagg ctagactaca
    21781 aaataatgcg ttgatgatgg aggcactgtg gaagcgatta agggatcgga cgttaatgtc
    21841 ccggatgcag tatttgcctg gatgctggat ggtagaggcg gcgttaaacc gctggaaaat
    21901 acagatgtga ttgatgaagc gcatccctgc tggctccacc ttaattatgt acaccatgaa
    21961 agcgcccaat ggctggcgac aacaccgctg cttcccaata acgtacgtga tgcgctggca
    22021 ggcgagagca ctcggccccg agtcagccgt ctcggtgaag gcacgctgat tacattgcgc
    22081 tgtataaacg gcagcaccga tgaacgcccc gatcaactgg tcgccatgcg tgtatatatg
    22141 gacgggcggt taattgtttc gacccgacaa cgcaaagtgc tggcgctgga cgatgtggtg
    22201 agcgatctgg aagagggcac gggtccgacc gattgcggag ggtggctggt ggatgtgtgc
    22261 gatgcgttga ccgatcattc cagcgaattt atcgagcagc tgcacgataa aattatcgac
    22321 cttgaagata atctccttga tcagcaaatt ccaccgcgtg gattcctggc tctgctgcgc
    22381 aaacaattaa tcgtgatgcg tcgctatatg gcaccgcaac gtgatgttta tgctcgtctt
    22441 gccagtgaac gtttgccgtg gatgagcgat gaccaacgcc gtcggatgca ggatattgcc
    22501 gatcgccttg ggcgcggtct tgacgaaatc gacgcctgta tagcacggac tggcgtgatg
    22561 gcggatgaaa tcgctcaggt gatgcaggaa aatttagctc gtcgtaccta tacaatgtca
    22621 ttgatggcaa tggtcttttt acccagtacc tttctgacag ggttatttgg cgtcaacctt
    22681 ggtgggatcc ctggcggcgg gtggcaattc ggattttcaa ttttttgtat tctgttagtt
    22741 gttcttattg gtggtgttgc tttatggttg catcgtagta aatggttgta acaaaagcaa
    22801 tttttccggc tgtctgtata caaaaacgcc gcaaagtttg agcgaagtca ataaactctc
    22861 tacccattca gggcaatatc tcccttgcag gtgaatgcaa cgtcaagcga tgggcgttgc
    22921 gctccatatt gtcttacttc cttttttgaa ttactgcata gcacaattga ttcgtacgac
    22981 gccgactttg atgagtcggc ttttttttgc ctgttattta tcagcgtcta ccctttaaga
    23041 gtccacccaa tgaccggagg gaaatatgac gacacttatt tatttgcaaa ttcctgtccc
    23101 tgaaccgatt cctggcgatc ctgttccagt gcccgatccg atccctcgcc cgcaacccat
    23161 gcctgaccca ccacctgatg aagaaccgat taaattgtcg catcgtgagc gtagatctgc
    23221 gaggatacgc gcctgctaac tttgcgtcga tgaccacgag aatagattgt gaccgctttt
    23281 tctaccctga atgttttgcc tcccgcccaa ctcacgaacc ttaatgagtt gggttattta
    23341 accatgacgc cggtgcaggc cgccgcgctt ccggcgatcc ttgccggaaa agatgttcgc
    23401 gtgcaggcga aaaccggcag cggcaaaacg gcggcttttg gcctcggctt gttacagcaa
    23461 attgatgcgt cgctatttca aacccaggct ttagtgctgt gtcctacgcg tgaactggcg
    23521 gatcaggtgg caggtgaatt gcgtcggctg gcgcgttttc tgccaaatac caaaattttg
    23581 acgttgtgcg gtggtcaacc gttcggtatg cagcgtgatt cgttgcaaca tgcgccgcat
    23641 attatcgtgg caacgccggg tcgtttgctg gatcacttgc aaaaaggcac ggtatcactg
    23701 gatgcgctga atactttggt gatggatgag gccgaccgca tgctggatat gggatttagc
    23761 gatgccattg atgatgtcat ccgttttgcg cctgcatctc gacagacgct tctgttttcg
    23821 gcaacctggc cggaagccat cgccgcaatc agcggacgag tacaacgcga tcctttggcg
    23881 attgaaattg actcaacaga tgctttgcca cccattgaac aacaatttta tgagacatcc
    23941 agcaaaggca aaattcctct gttgcaacgg ttattaagct tgcatcagcc atcctcttgc
    24001 gtggtgtttt gcaataccaa aaaagattgc caggctgtct gcgacgcgct gaatgaagta
    24061 gggcaaagtg cattgtcgtt acacggcgat ctggagcaac gcgatcgcga tcagactctg
    24121 gtacgttttg ctaacggtag cgcccgtgta ctggttgcta ctgatgttgc tgcgcgtggt
    24181 ctggatatta aatcgcttga gctggtggtg aactttgagc tggcgtggga ccctgaagtt
    24241 catgtacatc gcatcggtcg tacagctcgt gcaggaaata gcggtctggc gatcagtttc
    24301 tgtgctccgg aagaagcaca gcgggccaat atcatttctg acatgttgca gataaaactt
    24361 aactggcaaa cgccgccagc taatagttcc attgtgccgc tggaagcaga aatggcaacg
    24421 ttgtgtatcg atggcgggaa aaaagccaaa atgcgcccgg gtgatgtatt aggtgcactg
    24481 acaggagata tcgggcttga tggcgcagat attggcaaaa tcgccgtgca tccggcgcat
    24541 gtctatgtcg cggtccgtca ggctgttgct cataaagcat ggaaacagtt acagggcggg
    24601 aagattaaag gaaaaacgtg ccgggtgcgg ttattaaaat aatgaaatgt tgaattgccg
    24661 ggtgcaagag taaacatctt attcgggatt gccggatgcg acgctggccg cgtcttatcc
    24721 ggcctccata agagtagccc gatacgcttg cgcatcgggc gctatcctgg ttatttcact
    24781 tcaaccacat tcagtcgtaa ctcatccaac tgattttcat cttcttctgg ctgccagccc
    24841 gccggttgta gtgggatctc ttcgcgatca aacgccagat cacccccgtt aaccacttca
    24901 gaaccgtggg taatgccttt aaaatcgaac aggttggtat cgcacagatg cgacggcacc
    24961 acattctgca tcgcgctgaa catcgtctcg atacgccctg gataacgttt atcccagtca
    25021 cgcaacatgt cagcaatcac ctgacgttgc aggttaggct gtgaaccgca caggttgcac
    25081 ggaataatcg ggaacgcttt tgcatcggca aatcgctgaa tatctttctc gcggcagtag
    25141 gccagcggac gaataacgat atgtttgcca tcatcgctca tcagtttcgg aggcatacct
    25201 ttcatcttgc cgccgtagaa catatttaag aacaacgttt gcaggatatc gtcacgatgg
    25261 tgacccaacg cgatcttcgt cgcccccagt tccgttgcgg tacgataaag gataccgcga
    25321 cgaaggcgag aacacagtga gcaagtggtt ttgccctctg gaatcttctc tttcacgata
    25381 ccgtaagtat tctcttcaac aatcttgtac tcaacgccca gcttttcaag atactcgggc
    25441 agaacgtgtt ccgggaagcc cggttgcttt tgatcgaggt taacagccac cagcgaaaaa
    25501 ttgattggcg cgctttgctg caaattgcgc agaatctcca gcatggtata gctgtcttta
    25561 cccccggaga ggcaaaccat gatgcgatcg ccttcttcaa tcatattgaa gtcagcaatg
    25621 gcttcgccca cgttacgacg cagacgtttt tgtaatttgt tcaggttgta tatctctttc
    25681 ttcgtattct tttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt
    25741 gggggcaaac tttgcaacta cgataaccgc gcattcaaca tggctacctg ttcgtcgttc
    25801 atgtcatcaa tccacatacc gtaaatttca tacaccatct gcgcagtttc atgtcccatc
    25861 tggctggcta taaacgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgc
    25921 cgcgtatggt acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctc
    25981 gcacccaaac tgcttaccga gtaataaggt ttctgttttc cgttacacac tctgggcatg
    26041 aaaacaaaat gcagtttttg cttttcggtt ctgccgtact cacgatgata aaaagtgatt
    26101 tcgcttttgc gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aatagcaggc
    26161 cgcagtagtg ttaccgtccg aatccccgca tttgtttttg ggggaccgaa catatcaagt
    26221 atcgtcaggt ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc
    26281 agagcagcca gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttctgg
    26341 ctctgtcctt tttcactttc cattaatgca ttgaattctg ttttagataa cggatcaggc
    26401 tttattctgt tccgctgtaa tttttttact ccttcaaatg gtttggttga tataaatccc
    26461 gactgatacg caaaacgtaa caacgaacag agcagggcga tatagttatc aactgtgcgc
    26521 acggttcttc cttttttgtt ggatcttgga ttatccaggt aaagcgtttc tccatgcagc
    26581 agttcatttc ggtagtttaa gatatcgcta taacgaatat gtgatattgg ggtactctca
    26641 caaattatta tcctgagtgt ttttaattgt gatttcgttt tcttcattgt gtttgttgtt
    26701 aactctgtct ctttaatttt tgtccagata tcacaaagct ccccgaacgt ttttatgacc
    26761 ctcgttgtca ccatttttgc cccagtgctg gactggggga aacgtcttaa atactcaaat
    26821 tcaccggaat tgatttcatg aactatcaac gctcttaaat tcccggcttt tttaatgtta
    26881 ctgttagtga cctcccagcc tttcaatgtt tcccgacatc gttttcctcg aaacatgaac
    26941 cagatgcgaa tgtatttacc tcgaatctcg acacctgttg gtaatttaga catatcatga
    27001 gtctttgata aactgattta tctttggata gttgtaccag ataatccctc gcttactgtc
    27061 tggcttccct aaaggagata ctcgtttgaa gtggaagcct tccacccaac agttctggcg
    27121 gtatgcttca atttgtctgg cccccagacc agtgcgaagc atcaggccgt attcaaccat
    27181 ccactcttca ttaaagatta cttgtgccat cgcatcacct ctggcaggcg ccaatgttag
    27241 actgaaattg acgcccgatg ttgattatta ataatcagct atgaagtttt aatttgaata
    27301 caatgcaatt cacgaggact gaagtttctc gcaattaaaa tttatcagtt ttactttctg
    27361 ctctctggaa acgcctgctt cttttttacc tgagagcatt ttttcgcatt ctgatttcgt
    27421 tagtttagat tttgaatatc ttgtccagtt agtaggagtg ccaccttcct tttcaatagt
    27481 ggcggtaatt ttatacatga acacctctat tattatttcc agtggttcgt ttattccatc
    27541 tttcgagtgc ttctttttca cttccaccat aaccggttcg ggattcgcat ccgttacact
    27601 tcgctcggta atatcctgaa atgtctttca ccgttactga tggacaacca caaaatggac
    27661 atggtttgac ttttgtgtat cgcactggtt tttctcctgt gagctttgtg gctgcgctca
    27721 tttccattgc tccccaaata caaaaccaat ttcagccagt gcctcgtcca ttttttcgat
    27781 gaattctggc accatctcgt caaaactcgc catgtacttt tcatcccgct caaccatgac
    27841 ataatgcagg ccttcacgct tcatacgcgg gtcatagttg gcaaagtacc aggcatcttt
    27901 ttgcgtcacc cacatgctgt actgcacctg ggccatgtaa gccgacttta tggcctcgaa
    27961 accaccgagc cggaacttca tgaaatcccg ggaggtgaac gggcatttca gctcaaggcc
    28021 gttgccgtca ttgcataaac catcgggaga gcaggcggtg cgcatagtct cgtcgcgata
    28081 gatgatcggg gattcagtaa catccacgcc ggaagtaaac tcaaacaggg ctctggcgtc
    28141 gctctcgtac tgttttcccc atgccagcgc cttagcgtta acttcaggag ccacaccggt
    28201 gcaaacctcg gcaagcaagg tgtggaagta ggacattttc atgtctggcc acttttttcc
    28261 tgagcggggt tttgctatca cgttgtgaac gtctgaagct gtgatgacac ctagccgtaa
    28321 tttgtgccac gcatcatccc cctgcacgat agctctcacg tcgatcccgg tacgctgcag
    28381 gataatgtca ggtgtcatgc tgccaccttc tgccctgcgg ctttctgttt caggaatcca
    28441 agagctttta ccgcctcggc ttgtgtcagt tctgacgatg cgcgaatgtc gcggcgaagt
    28501 atctgggaac agagcggcaa taagtcctca tcccatgttt ttcccagggc gattagcaga
    28561 gcgttaattt cctgcatggt ttcatcgtta accggagtga tgtcgcgttc cggctgatgt
    28621 tctgcagtgc atgcggtatt ttcgacaatg cgctc
//
LOCUS       DAHBHC010000067        28004 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHBHC010000067 DAHBHC010000000
VERSION     DAHBHC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.067"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..76)
                     /locus_tag="M2A35_003453"
     CDS             complement(<1..76)
                     /locus_tag="M2A35_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HCB7199704.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGV"
     gene            complement(88..366)
                     /locus_tag="M2A35_003454"
     CDS             complement(88..366)
                     /locus_tag="M2A35_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199705.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGNEPYIEIICMDSEAEIQKAD"
     gene            complement(377..715)
                     /locus_tag="M2A35_003455"
     CDS             complement(377..715)
                     /locus_tag="M2A35_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCB7199706.1"
                     /translation="MLKQRRNFRTGTERHANRFTTSASRSNIRYSLSDTHATPDGHTV
                     KQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKKTDL"
     gene            complement(730..1008)
                     /locus_tag="M2A35_003456"
     CDS             complement(730..1008)
                     /locus_tag="M2A35_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCB7199707.1"
                     /translation="MAKVLNTHEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     AVNTVKRQADRGSIPINQDEKNSRRTVNLFALFLKTIRSAEKYVQMTK"
     gene            1105..1413
                     /locus_tag="M2A35_003457"
     CDS             1105..1413
                     /locus_tag="M2A35_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7199708.1"
                     /translation="MLIGEKIRVIRESEDLTREEFCGLIDVPIGTLRRYETGRIENIG
                     GEVLIKIVNHPRFFKYMNWLMTGKTNEAAGQISPSLSPDGPKSTSPSQKPRKTGTQPG
                     "
     gene            1502..2461
                     /locus_tag="M2A35_003458"
     CDS             1502..2461
                     /locus_tag="M2A35_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7199709.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAIDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKQVFPTLDYPLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGDSNGNKSPH"
     gene            2439..2921
                     /locus_tag="M2A35_003459"
     CDS             2439..2921
                     /locus_tag="M2A35_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199710.1"
                     /translation="MEINHPTDEELRDFLLKKYLEEQFQNEINGSNQKDIKQHEELLK
                     EVTPEIFFRFLSEKGVSGACISCGSSKLSAPENRVICIDKIPDDFKKLTKEEQHNVVK
                     GLAQRYVAYVAFGDTNTPSGVSSYYMVHCLNCGYLSLYRTSEVLKWLEKNNVQDDNNG
                     "
     gene            3022..3492
                     /locus_tag="M2A35_003460"
     CDS             3022..3492
                     /locus_tag="M2A35_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HCB7199711.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKCDIANLKGELKSDTANLKEQLKSDINSLKGELT
                     EAMDKRFDKIMDEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAYFVAKFVG"
     gene            3685..4665
                     /gene="btuC"
                     /locus_tag="M2A35_003461"
     CDS             3685..4665
                     /gene="btuC"
                     /locus_tag="M2A35_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCB7199712.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            4728..5279
                     /gene="btuE"
                     /locus_tag="M2A35_003462"
     CDS             4728..5279
                     /gene="btuE"
                     /locus_tag="M2A35_003462"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCB7199713.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            5279..6028
                     /gene="btuD"
                     /locus_tag="M2A35_003463"
     CDS             5279..6028
                     /gene="btuD"
                     /locus_tag="M2A35_003463"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCB7199714.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            6106..6570
                     /locus_tag="M2A35_003464"
     CDS             6106..6570
                     /locus_tag="M2A35_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7199715.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            6817..7530
                     /gene="ydiV"
                     /locus_tag="M2A35_003465"
     CDS             6817..7530
                     /gene="ydiV"
                     /locus_tag="M2A35_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCB7199716.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            7593..9029
                     /locus_tag="M2A35_003466"
     CDS             7593..9029
                     /locus_tag="M2A35_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCB7199717.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(9033..9224)
                     /gene="hemP"
                     /locus_tag="M2A35_003467"
     CDS             complement(9033..9224)
                     /gene="hemP"
                     /locus_tag="M2A35_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCB7199718.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(9356..10402)
                     /gene="aroH"
                     /locus_tag="M2A35_003468"
     CDS             complement(9356..10402)
                     /gene="aroH"
                     /locus_tag="M2A35_003468"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCB7199719.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNQRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(10559..11392)
                     /gene="ppsR"
                     /locus_tag="M2A35_003469"
     CDS             complement(10559..11392)
                     /gene="ppsR"
                     /locus_tag="M2A35_003469"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCB7199720.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            11725..14103
                     /gene="ppsA"
                     /locus_tag="M2A35_003470"
     CDS             11725..14103
                     /gene="ppsA"
                     /locus_tag="M2A35_003470"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCB7199721.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGAALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(14160..15806)
                     /gene="fadK"
                     /locus_tag="M2A35_003471"
     CDS             complement(14160..15806)
                     /gene="fadK"
                     /locus_tag="M2A35_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCB7199722.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCVRLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(15863..16156)
                     /locus_tag="M2A35_003472"
     CDS             complement(15863..16156)
                     /locus_tag="M2A35_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCB7199723.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(16153..17442)
                     /locus_tag="M2A35_003473"
     CDS             complement(16153..17442)
                     /locus_tag="M2A35_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCB7199724.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(17498..18436)
                     /locus_tag="M2A35_003474"
     CDS             complement(17498..18436)
                     /locus_tag="M2A35_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCB7199725.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(18456..19220)
                     /locus_tag="M2A35_003475"
     CDS             complement(18456..19220)
                     /locus_tag="M2A35_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCB7199726.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASGIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            19536..20447
                     /locus_tag="M2A35_003476"
     CDS             19536..20447
                     /locus_tag="M2A35_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCB7199727.1"
                     /translation="MYQRCFDNASETLFVGGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(20490..21641)
                     /locus_tag="M2A35_003477"
                     /pseudo
     CDS             complement(20490..21641)
                     /locus_tag="M2A35_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(21655..23250)
                     /locus_tag="M2A35_003478"
     CDS             complement(21655..23250)
                     /locus_tag="M2A35_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCB7199728.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(23394..24152)
                     /gene="aroD"
                     /locus_tag="M2A35_003479"
     CDS             complement(23394..24152)
                     /gene="aroD"
                     /locus_tag="M2A35_003479"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCB7199729.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(24183..25049)
                     /gene="ydiB"
                     /locus_tag="M2A35_003480"
     CDS             complement(24183..25049)
                     /gene="ydiB"
                     /locus_tag="M2A35_003480"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCB7199730.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(25061..26326)
                     /locus_tag="M2A35_003481"
     CDS             complement(25061..26326)
                     /locus_tag="M2A35_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199731.1"
                     /translation="MSQYKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(26553..27767)
                     /locus_tag="M2A35_003482"
     CDS             complement(26553..27767)
                     /locus_tag="M2A35_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7199732.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(27867..>28004)
                     /locus_tag="M2A35_003483"
     CDS             complement(27867..>28004)
                     /locus_tag="M2A35_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1870 family protein"
                     /protein_id="HCB7199733.1"
                     /translation="GNNTMRFFPDLTAFQQVYPDGNFIDWKIYQSVAAELYAHDLERL
                     C"
BASE COUNT         7106 a         6704 c         7014 g         7180 t
ORIGIN      
        1 gaacgcctga tgcgcttaag cgaaaataaa aacgctcaag tgctttgttg atgagtgttt
       61 tatattcttt gtccattgca acacccttta atctgctttc tggatttcag cttctgaatc
      121 catgcaaata atttcgatat agggttcatt gccattaacc tgacgtgcct tttcagcttc
      181 gctaatgatt tcgcgtacag tctggtacgg aagttctacg gtcaggcgcg tgccgttcag
      241 ataaacgtaa gtagctgcat ttttttctga tgggacgact ccatcaatag ctgatgcgcg
      301 taataacagt tcaccgcgaa aatcaataaa acggataaat acaccttgtg catgctcttt
      361 ggtcataaag cacctgttat aaatcagtct ttttaataaa actttgcccg cgaagcagac
      421 gatcaaccgt gcgaagtgct tcgtataatg tgaaatcctg cccgaactga ttgtcaccgc
      481 agctcaatgc aaaaatgcgg tttccggtaa acggattgcg tgggcatttg tggactacga
      541 ttcctgcttt ctcaatcaac caggcgtgtt cgccaatttg ttttactgta tggccatccg
      601 gcgttgcgtg tgtatcactc aggctgtagc ggatgttgct gcgtgatgca ctggtagtga
      661 aacggttagc gtggcgttct gttccggtac gaaaattacg gcgttgcttc agcataaaat
      721 gacacctcgt tattttgtca tctgcacgta tttttctgcg ctcctgattg ttttcaggaa
      781 aagagcgaag agatttactg tgcgtcttga gttcttttca tcctggttga tgggaattga
      841 acctctgtca gcctgtcttt tcactgtgtt aacagcttgg ttggtacgct ttgcgtaatc
      901 tttcaggctt tcttcaagta ctggtaaccc atgctcatcg cggtagggat agaacgctgc
      961 taaacgctca aagtctgcct gttcgtgtgt gttcaggact tttgccatgt gtgataacct
     1021 gcgctatctg tggttgtttg tgacttggtg tacttataag tacaccttgt gcgcaagctt
     1081 agtgtactta taggaacact gtcaatgctt atcggtgaaa aaattagagt gattcgtgaa
     1141 tcagaggatt taacgcgcga agaattttgc ggcctgattg atgtgcctat cggcactttg
     1201 cgtcgttatg aaacggggcg gattgaaaac atagggggcg aagtgcttat caagattgtt
     1261 aatcaccctc gcttttttaa gtacatgaat tggcttatga cgggaaaaac aaatgaggct
     1321 gctgggcaga tcagtccgtc tctctcccct gatgggccaa aaagcacatc gccttctcaa
     1381 aaaccccgca agactggcac acagcccggc taatcatgga gcgctggggg catggtggtc
     1441 ttgtaacgct ggggtttcac gaatgagcat aaaatcaatt ccgggagggt atcttcttga
     1501 catgcgcccg gaggggcgta aaggcaaacg cattcgcaaa aaatttaaaa cgaaatcgga
     1561 tgcagtttta tatgagcggt gggtgctggc gcaacagcat aacaatgagt ggaaaggaaa
     1621 ctccattgat cgccgtccgc tgtcagtgct tattgacttg tggtggaaat accacggcca
     1681 gctaatgaag tcagggcata acacgcgcct taaattgctg cgcttgagtg aggcaattga
     1741 tgacccgtgc gtgcataaac ttaatacaac gatgctcacc gagctacgtg tgtccaggat
     1801 agagcagggg atacagccca gcaccataaa tcgagagatt ggggcgttaa gcgcgatgtt
     1861 taccgcactc atctcatccg gccattttct taacgataac cccgttcaag gccttaaagg
     1921 aatgaaggtt aacgagcgcg aaatgggata tctgagtaag tctgaatgtg ttcagttgct
     1981 ggatgcactg gctgaaaatc ccgatgaacg gctggctgtc gaaatccttc tgtcgaccgg
     2041 ggcgcgatgg ggcgaggtag cggcactgga gcagcgccgt gttcttcatt gtcgaatcac
     2101 tttttcaaaa acgaagaaca gcaaaaaccg taccgttcct atttctgaaa gcctgtttga
     2161 aaagatcaaa aaacggggcg ggaaacaggt gtttccgacg ctggattatc cattggttcg
     2221 cgatgtcatc aaaacggtcg cacctgatgt tcctgacggc caggctgttc atgcgctgcg
     2281 ccacaccttc gccagtcatt tcatgatgaa cggcggcaat attctgacgc tccagaaaat
     2341 tctggggcac gcaaagattc agacaacgat gatttatgcc catcttgcgc cggattacct
     2401 gcaggatgcg gtgaggttta acccactagg agacagcaat ggaaataaat caccccactg
     2461 atgaagagct aagggatttc ctgttaaaaa aatatctgga ggagcaattt cagaatgaaa
     2521 tcaatggatc taatcagaaa gatataaaac aacacgaaga gttgctaaag gaggttacgc
     2581 cagaaatatt ctttcgcttc ttgtctgaga agggggtttc tggtgcgtgc atttcgtgcg
     2641 ggtcatcaaa gctatcggct cctgaaaaca gggttatttg tatagataaa ataccagatg
     2701 actttaaaaa acttacgaaa gaagagcaac acaatgtcgt taaaggactc gcgcagcgtt
     2761 atgtagctta cgttgctttt ggtgatacaa atactccttc aggtgtgagt agctattaca
     2821 tggtgcactg tctgaattgt ggttatctga gcctttaccg gacttctgag gtgctgaagt
     2881 ggctggagaa aaacaacgtg caggatgata ataatgggta atgcggcaga aaatcttttt
     2941 ggtggttcag caaatgtttt atcgcatccg aggcgtgatg tactttatca tggtggcgat
     3001 ggcggcagta gtggaggcgg aatgtcagat aaacttgaaa ggcgaattga gcggctcgaa
     3061 ggtgatttat cgctaacaag aaacgacctt gcgacacttg ctgaacgcac tacaaacctc
     3121 tcaactaaag ccgatgttgg tgaggtgaaa ggtgaactca aagcagacat agcacatctg
     3181 aaaggtgatc ttgaatgcga tattgcgaat ctgaaaggtg agcttaaatg tgatattgcg
     3241 aatctgaaag gtgagcttaa atctgataca gctaacctga aagagcagct caaatcagac
     3301 attaacagcc tgaagggtga gcttaccgaa gcgatggata aacgctttga caagattatg
     3361 gatgagatga atcggcggtt tgacaaggtt gatgataata cgaagtggcg ttggagtggc
     3421 attattgtgc cagtgtgcac aaccattttc acggcggcgg tcgcgtattt tgttgctaaa
     3481 tttgttggct gatgatccac aaagtgacca catccctgtt acttgctgtg gttggctgtg
     3541 tttttgtgtg tctgtaagct attgataatt atgtaactta ttgatttttg aatgtaatta
     3601 tggccgctct gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca
     3661 tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca gcgacaaaat
     3721 attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt aagcctttgc
     3781 gccggtgaac aatggatctc gccaggtgac tggtttactc ctcgtggcga actgttcgtc
     3841 tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc gctggctata
     3901 tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg actacttggc
     3961 gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca agggcaactc
     4021 cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac tttaatactc
     4081 ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg cgttgcatta
     4141 gggattatct gtagcgcact aatgacgtgg gctatctact tttccacctc tgttgattta
     4201 cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg gcaaagctgg
     4261 ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag gccgatgaat
     4321 atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccctgtg gttctggcgc
     4381 aatgtgctgg tggccgcgac cggctggatg gttggcgtca gtgtggcgct ggcgggtgct
     4441 atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt aaccgatcat
     4501 cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct ggccgatatt
     4561 gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac cgcaacgttg
     4621 ggtgcgccgg tgtttatctg gttattgtta aaagcaagac gttagccgca aaaagacggt
     4681 ctatgattaa aagctaagat ttttactgac cacacccagg agaaacgatg caagattcca
     4741 ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag aagtacgctg
     4801 gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg cttaacgccg caatatgagc
     4861 agttggagaa tattcagaaa gcctgggccg atcgaggttt tgtggtgctg ggattcccgt
     4921 gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact tactgtacca
     4981 ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc gaaggacgcc
     5041 atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg gaagagagcg
     5101 gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct gtaccctgat gatattttat
     5161 ggaattttga gaaattttta gttggcaggg acgggttggt catccagcgt ttttccccgg
     5221 atatgacgcc tgaagatccc attgtgatgg aaagcattaa actggcgttg gcaaaataat
     5281 gtctattgtg atgcagttac aggatgttgc ggaaactacc cgcctggggc cgctttctgg
     5341 cgaggttcgg gctggggaga tcctgcacct ggtggggccg aatggcgcgg gtaagagtac
     5401 cttgcttgca cgaatggccg gaatgaccag cggtaaggga agcattcagt tcgcggggca
     5461 accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctacc tttcacaaca
     5521 gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc agcacgataa
     5581 aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg acaaactcgg
     5641 acgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg ctgcggtggt
     5701 gctgcaaatc acaccacaag ccaatcccgc aggtcaattg ctgcttcttg atgagccgat
     5761 gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg ctctgtgtca
     5821 gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacattgc gtcatgcgca
     5881 tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag aagaggtgct
     5941 cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg atatcgaagg
     6001 tcacagaatg ctgatttcga ccatctgatt tcattagtat cttgaaaatc gggtaattac
     6061 gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg tttctgcctt
     6121 attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc gccgccaaat
     6181 gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct acaaagctgg
     6241 catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg ttcgggattt
     6301 gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc cccgagaaac caaagaacaa
     6361 gcctctatcg gcacgcaaat tgataaagac gagttgctgc ctggtgacct ggtctttttc
     6421 aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa caaccaattt
     6481 atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt ctattggcag
     6541 aaaaatttct ggcaggcgag acgaatttag tatgtcgaaa aatggacggc gatgatacat
     6601 cgccgtccat ttactcctga atacagtagt attttgcaga caaaaattcc gtaaatggat
     6661 tattcgttta taatcaggat aatctaaaaa aaggacccct gaaaaagtgg ttaacatgga
     6721 acaaatgaaa gttactcctt ctgtaattgt tatgtcttat ggcctcatcc cttttagccg
     6781 gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt ggagaatctt
     6841 tatcattctg attgctattt cctgccgata agagataacc agcaactcct tgtcggcgtt
     6901 gagttaatta cccatttctc cagtgaagat ggcacggtgc gtatcccgac gagtcgggtt
     6961 atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt ggaattacta
     7021 aaatcatgcc aacatttttt tattcaacat aaactctttg cctggcttaa tttaacgcca
     7081 caagtggcta cattattatt agagagggat aattacgcag gcgaactatt aaaatatccg
     7141 tttattgaac tgttgattaa tgagaattac ccgcatctca atgaaggaaa agataatcgc
     7201 ggtcttttat ccttatcgca agtgtatccc cttgtgttag gtaatttggg agcaggcaat
     7261 agcacaatga aagcggtttt tgatggtctt ttcacccgcg tcatgctgga taaaagtttt
     7321 attcagcaac aaatcacgca ccgctccttc gaaccgttta ttcgtgcgat ccagtcgcaa
     7381 atttcccctt gttgcaattg catcattgcg ggaggcattg atacggcaga aatattggca
     7441 cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc cgttcccata
     7501 aatcagataa cgacgttggt tcagcgataa cccttctgtt tgctggtgtt taagacgaga
     7561 gtaaccgtct acactatcaa acaggaggat ctatgaccct gtcttttatt acccgctggc
     7621 gcgatgaatt gccagaaacc tatacagcac tttcccctac gcctttaaat aatgcccggc
     7681 tgatttggca taataccgaa ctcgctaaca cgctgagtat tccatcgtcg ctgtttaaaa
     7741 atgctgcagg tgtctggggc ggcgaaaccc tactgcctgg tatgtcacca ctggcgcagg
     7801 tttacagtgg tcatcagttc ggcgtctggg cgggccaact gggtgatggg cgcggcattt
     7861 tacttggcga acaactgctt gctgatggca ctacaatgga ctggcatctg aaaggtgctg
     7921 gcctgacgcc ttattcgcga atgggtgatg gacgggcggt tttacgttcg acgatacgag
     7981 aaagtctcgc cagtgaggcg atgcattatc tgggcattcc gacgacccgc gcgttaagta
     8041 tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga gccaggcgcg atgctgatac
     8101 gtgtggcacc aagtcatctg cgctttggtc atttcgaaca tttttactat cgccgcgagc
     8161 cggaaaaggt tcgtcagtta gctgactttg ccattcgtca ttactggtca catcttgaag
     8221 atgatgagga caaataccgt ctctggttta acgatgttgt cgcacgtacc gcatcgttaa
     8281 ttgcccaatg gcagacggtc ggctttgctc atggggtgat gaataccgac aacatgtcgc
     8341 tgctggggct gacgcttgat tacgggccgt ttggttttct tgatgattac gaacccggtt
     8401 ttatttgtaa tcactcggat catcaagggc gttacagctt tgataatcaa cctgccgtcg
     8461 cgttgtggaa tttacagcgt ctggcgcaga cattgtcacc atttgttgcc gtagatgccc
     8521 tgaatgaggc cctggacagc tatcagcagg ttttgttgac gcattatgga caacggatgc
     8581 ggcagaaact gggcttcatg acggagcaaa aagaggataa cgcgctactg aatgaattat
     8641 tcagtctgat ggcgcgagag cgcagcgatt atacccgcac attccgcatg ctgagtctga
     8701 ccgagcagca cagcgcggcg tcaccgctac gtgatgagtt tattgatcgt gcggcatttg
     8761 atgactggtt tgcccgttat cgggggcgtt tgcaacaaga cgaggttagc gatagtgagc
     8821 gtcagcagct gatgcaaagc gttaacccag ctctggtgtt gcgcaactgg ttggcgcaac
     8881 gggcgattga agcggcagaa aagggtgata tgacggaatt gcaccgcctg catgaggcgt
     8941 tgcgaaatcc tttcagcgac agagctgatg actatgtcag tcgtccacct gactggggta
     9001 aacggctgga agtcagctgc tcgagttaaa ggctacttgg tcaacagcag cttgccagcc
     9061 tgagttttac ggagcagata ttcttgcccg tcatgatcga taatcagttt gccatccgga
     9121 cctaacagtg tctggctgga aattcgccga ataggctgcg gggaagcggt cttgtgattg
     9181 gcatccgttt caggcgtgag ttttctgcta tccgtataac gcataaaaat taataataat
     9241 tgatataaca atgattctta ttatcaatat tatgactgac cagagcaagc agtaatgtgc
     9301 tgagaagaca acaagttgcg cagaaatgcc catcccatca ggaatgggca cgcattcaga
     9361 agcgggtatc taccgcagag gcaagttttt cgaccaggcg ttcggtatct tcccagccca
     9421 gacacgggtc ggtaatggat tgaccgtaag tgagcggctg accgccgacg attttttgcg
     9481 ttccttcgcg caggaaactt tccgccataa ttccagcaat cgccgtagag ccattgcgga
     9541 tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc tggcagttac
     9601 cgtggctgaa atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc gtatcgcagg
     9661 ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata ataatgtggc
     9721 catacgggtt gccgctggtc tgatagatgg tcatctgacc atttttgtct ggcgagagaa
     9781 acatatggct ggcgcgggct gcgcggatag catccacggc aatccgctgg ttgccatcgg
     9841 taccattttt aaaacctacc ggacaggaga gtgccgaagc catttcacgg tggatctgac
     9901 tttcggtagt acgtgcgcca atcgcgcccc aactgattaa atcagcaata aactgaccgg
     9961 tcaccatatc gaggaactca gtcgcggtag ggacacccag ctcatttacc tgtaaaagta
    10021 atttgcgcgc cagctccaga ccgtgattta cccgatagct gccgtttaaa tccggatcgg
    10081 agattagccc tttccagccg acaacagttc gtggtttttc aaaataggtg cgcattacga
    10141 tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg tactccattg
    10201 cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc ttatcttcac
    10261 cattcagtat tttttcaatt ctgcggcggg agtcggtgac atgggtggcg acgccaggcg
    10321 ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata cgcgcagtac
    10381 ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta cggcaggact
    10441 accgggaagt gatgcgcatc acgataaacc aatcgcagct gatttcaagt gcgaggtgtg
    10501 tcacacctcg ccgggtggtt agcatgataa caaaaaaata agctaatgca ctagttctct
    10561 agtacattcg gcggctaagc cccatgatat cgaggatctt ggtggcaatc tcttctaccg
    10621 aataattggt actgttaatc cacgggatct gatttttacg gtacaacgct tctacttccg
    10681 cgacttccat cctgcactga cgaagcgagg catagcggct gttctcccga cgttcctcgc
    10741 gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc tgaagcggtt
    10801 tgagcgacgc gggtagcacc agattgtcca tatcgtcggc aataaagggg tagtttgccg
    10861 cgcggatacc aaactgcatt gccagataca gactggtggg ggttttacca cagcgagaaa
    10921 caccgagcag gatcacctga gcctggtcca gattgcgcaa cgaaatgccg tcatcgtggg
    10981 cgagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta ttagggttaa
    11041 ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt tgtagcgggg
    11101 caaccagcgc ctgaacaata tcctggcaaa agccttcact ttgcaagatg atggcgcgaa
    11161 tctccggcaa cacgatggag tagaagacca gcgggcgcac acctgtctgg tgataaattg
    11221 cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac ggcagcgtaa
    11281 tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc tccgcagtta
    11341 ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc attttgttct
    11401 tcccgtgatg cagacataat tctatgaaag cataaattaa aaacgcacag aagcgtagga
    11461 cgtaatgtct ggtttataaa atgaaccttc aattttattt tttatgaaaa cagcatttca
    11521 tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaatag agcaggtttg
    11581 cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac gattcaccgt
    11641 ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc tcaaaccgtt
    11701 catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct ggtgctttgg
    11761 tataaccaac tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc ctccctgggt
    11821 gaaatgatta ctaacctttc cggaatgggt gtttccgttc cgaatggttt cgccacaacc
    11881 gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat ttatgaactg
    11941 ctggataaaa cggatattga cgatgttacc cagcttgcga aagcgggcgc gcaaatccgc
    12001 cagtggatta tcgacactcc cttccagcct gagctggaaa acgccatccg cgaagcctat
    12061 gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tgcgctcctc cgccaccgca
    12121 gaagatatgc cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa cgttcagggg
    12181 tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa cgatcgcgcc
    12241 atctcttatc gtgtgcacca gggttacgat caccgtggcg cggcgctctc cgccggtgtt
    12301 caacggatgg tgcgctctga cctcgcatca tctggcgtta tgttctccat tgataccgaa
    12361 tccggctttg accaggtggt gtttatcact tccgcatggg gccttgggga aatggtcgtg
    12421 cagggtgcgg ttaacccgga tgagttttac gtccataaac cgacactggc ggcgaatcgc
    12481 ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta cgcgccgacc
    12541 caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg tgacatcttc
    12601 tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat tgagaaacac
    12661 tacggtcgcc caatggatat tgagtgggcg aaagatggcc acaccggtaa actgttcatt
    12721 gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg ttatacgctg
    12781 cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat cggcgcgggt
    12841 ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg cgacgtgctg
    12901 gttactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc tgccatcgtc
    12961 accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact gggcattccg
    13021 gcggtagtgg gctgtggaga tgcaacagaa cggatgaaag acggtgagaa agtcactgtt
    13081 tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt tagcgtgaaa
    13141 agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa cgtcggtaac
    13201 ccggaccgtg ctttcgactt cgcctgcctg ccgaacgaag gcgtgggcct tgcgcgtctg
    13261 gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga gtttgacgat
    13321 caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga ttctccgcgt
    13381 gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc gttttatccg
    13441 aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa cctggtcggt
    13501 ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg cgcgggccgc
    13561 tatgtttccg acagcttccg cgactgcttc gcgctggagt gtgaagcagt gaaacgagtg
    13621 cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg taccgtagat
    13681 caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaaacgtgg cgagaacggg
    13741 ctgaaaatca tcatgatgtg tgaaattccg tccaacgcct tgctggcaga gcagttcctc
    13801 gaatatttcg acggcttctc aattggctca aacgatatga cacagctggc gctcggtctg
    13861 gatcgtgact ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc ggtgaaagca
    13921 ttgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg gatttgcggt
    13981 cagggtccgt ccgaccatga agactttgcc gcatggttga tggaagaggg gatcgatagc
    14041 ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga actgaagaaa
    14101 taaaataaat ccccggcggc gttcagtcgc cgggtttatg tgatccccga agatgaaact
    14161 tattcaatct cttcacagac atcctgcgtt aatcgccgca taatatcttt tcttaacaaa
    14221 aacttttgta ttttacctga ggcagtgcgc ggtagttttt cgattaccac gatatgttca
    14281 ggatatttat attttgcgac ccgtttacgg ctaaaaaaag ccaccacctc ttccagcgat
    14341 aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc taaacgttca
    14401 tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa aatatcttcc
    14461 acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt gcgtccggta
    14521 atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata ccagccttct
    14581 tcatccagcg cacgggcggt taattcaggt tcatcaaaat atcccataaa cacattgggg
    14641 ccacgcgagg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc gtcatcgacc
    14701 actttaatct ctacacctgc ggcagcgtaa ccatcggtgt gcataaagcg cgacaaagga
    14761 tcatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac acttaataat
    14821 ttaatgccga gctgctggca ttcacgcgcc acttttttgg ggattgtggt tccgccgcaa
    14881 agaaagaaac gcagcgctga caggtccgcg ggttgtttct ctagtacatt caaaagatca
    14941 tagacaaacg gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag cagcgcgaga
    15001 cacgcatcag gagtgaaaat atctaacaac acgctgcgag caccaattaa aaatggtgct
    15061 gttacgccat gcagaaatcc cgttgcgtga ccaagtggcg caggcatcat aaagacatcc
    15121 tgccaggtca gattcagccg cacgcaataa gcccgctcac tggcgagaat attgttatgc
    15181 gttagcatta cgccctttgg cagaccctcg gttccggagg taaacagcac cgcagctaat
    15241 tcatcgccgt gggtcgttat cgccgtggtc agtgaggtat tgtcggcgat aatctgactt
    15301 aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg ttgtagttgt
    15361 ggaagctgat tttgcagcgg caggattaaa tctaccgggc gcgtttgttt aaacaacgtc
    15421 ggtgcaaaga acatttttgc ctgacactta ttgagcaccc acaccagttc tgcttcccgc
    15481 caggaaggca acagcggcac ggaaaccgca ccgattttca ggcaggcaag atagataacg
    15541 gtaaattcac accagccagg cagttgaaat gcgatgcgat cgcctgattc aatacccttc
    15601 gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata ggtgtacgat
    15661 gcaccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt ctgctgccag
    15721 taatcggcca gcgaagcatc gccccatagc ccttgctgac gatacgccgc acgacgttgt
    15781 tcgttgaacg ttaatgtcac tttcatgttg gactctcgaa acagaacatc aggcccgaga
    15841 tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag gtgcctgcgg
    15901 gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg ccgcactcca
    15961 gacaaccggc agaatcaaaa tgaatgttgc cggcgtcatc ctgcttataa agtccggcag
    16021 ggcaggcttt cattaattta cggaattcat tgatatcggg atttgccgcc agaatgatat
    16081 gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg acgttaaccg
    16141 tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca agttgatcag
    16201 cccaattttc ttcgcatgtc ccatgatcat tttgcgtacc ggctggtttg gtttgccgtc
    16261 aatggtgaac atctcgttca tgatgtcggc gaccattcgt gggtattggc taaacaggcg
    16321 cgggttttcc atcagcgccg ggatcttgcg gaaatgctgc atatcgcgca tgacgcagct
    16381 ttgttccagc tcacgtttgt attgcgccag actgctggcg gagaaatcct cgcgttcttt
    16441 ggcggcgatc accgttgtgg cggcagcctg agccgatgca atggctaaat ccatgccacg
    16501 aactgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca tcacgccatc
    16561 gttaaccatc tgcggcacca ttgccagacc gccttctggc accatatgcc cggaatattc
    16621 aagcagtttg ccgccgctaa ttagtgggcg aatggcgggg tgttgtttaa aatcttccag
    16681 catttgcggc acgctttttt gcgcatgggc gatatcaccc aatccacaaa ccagccccaa
    16741 ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt cagaagggga
    16801 accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat cgttgatctg
    16861 ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg gatcggaagc
    16921 gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcagcca gaatcaccac
    16981 attcgcttcg agaatatcat ccccagcctg cacgccagtg accttgtttc cttcacgaac
    17041 cagcgcatcg acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg cttgttccat
    17101 caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgcg gaacatctgg
    17161 ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg gttaagaagg agattttctc
    17221 gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag cctggaatga ttgcttcaag
    17281 tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt cgccgcgttc
    17341 tatcaccagg acatccagcc cggctcgcgc catgaccagt gcggcaacgc taccagcaac
    17401 gcccgcaccg accacaatgg catcaaattt gtcttccgac atgcgttatg ctcccttccc
    17461 gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac tggctaatca
    17521 gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcataa ttgaagattg
    17581 gcgcattttt atctttattg atggcgacaa tcacttttgc gccgttgccg ccaaccatat
    17641 gctggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc aacacgccgg
    17701 agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt ggacgtgaac
    17761 agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg tcatcctgcg
    17821 ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc acgctgcttg
    17881 cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaaca taaggtaccg
    17941 tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca ccgggtgcaa
    18001 gggtaatgat cgccagcggg ctgttgatct tttcctgagc gaacgctaac ccgccataca
    18061 tccggtgttc ggcgcaaatg tgaccatcga taatatccac cgccgtggca tcgttcacca
    18121 gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg
    18181 ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt tcggcgtaat
    18241 tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt ggaccataag
    18301 gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt tggccccatt
    18361 gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag acccagacgc
    18421 tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt cttcagatgt
    18481 tccgccagct cggcaatggc ctccggcgaa tcgttatcga taatgatgtg cttacgttct
    18541 gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg gctccagtca
    18601 ataccacttg cctgccactg atttaccggt tttttacccg cgccgagaat ggctttcatc
    18661 gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg aacagagagt
    18721 tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc
    18781 tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac cagcaagcca
    18841 gcctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc gaagccgatc
    18901 ttttcaactg ctgctgccag cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc
    18961 gcatcctgca ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc
    19021 gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc atctgttgca
    19081 agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc gtcggcattg
    19141 tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg caccagctta
    19201 aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca tctccgatgg
    19261 aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggttttcg acggagcaag
    19321 ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatc gctaaaacaa aagtgcaatt
    19381 tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa taatcttgtt
    19441 tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat ccggcatata
    19501 ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt tttgataatg
    19561 ccagtgaaac gctgtttgtc ggcggtaaaa cgccacggct ttcacgtttt gcaattagcg
    19621 atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag ctgatttacg
    19681 tcaagaaagg ggtagcaagg tttaccatcg attcttcgtt atatgtcgca catgcggatg
    19741 acattgtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt aacgatccgg
    19801 caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag gaaaatcagc
    19861 tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa gtcataaaaa
    19921 ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa acatcttcgt
    19981 tatgggatgc atttgcctat acgttagcca ttctttacta cgaaaatttt aaaaatgctt
    20041 atcgttcgga gcaggggtat attaaaaaag atgttctgat aaaagatatt cttttctatc
    20101 tgaataataa ttatcgcgaa aaaatcactt tagaacagtt atcgaaaaaa tttcgtgcca
    20161 gcgtcagtta tatttgccat gaatttacca aagagtatcg tatttcccct attaactatg
    20221 ttattcaacg gcgtatgacg gaagcgaaat ggtcactcac taatactgaa ttatcacagg
    20281 cagagatctc ctggcgtgtg ggttatgaga atgtcgatca ctttgccaaa ctgtttttgc
    20341 gccatgtcgg ttgttcgccc agcgattacc gcaggcaatt taaaaactgt tttgcggaac
    20401 aagaaatcct atctgaattt cctcaaccgg taagtcttgc cgggtaaatc tggcggcgca
    20461 gtttgttaag aagctgcgcc gcctgcagat tatttgttct gataatcttt caggatctgc
    20521 cgacccgcaa cgtaaatcat aatttcgtct gtaccgccgc cgatacgttc acaacggaca
    20581 tcacgccaga agcgggagac gcgcgcctca tcggtatagc ccagaccgcc catgatttga
    20641 atcgcatcat caatgacttc cattgcggta cgtgcacagt acagttttgc cagcgccgcg
    20701 ctggtgcgca gtgactgatg ctgatcggct tgccatgcca ctttcagcac catgttgcgc
    20761 atgttgtcga tcttaatcgc catcagcgcc agtttttcct ggatcatctg gttatgacca
    20821 atgggcttac caaaagcgat acgttggttg gcatagcggg cggcatcttc aaaggcgcat
    20881 tcggcaaagc cggtgctgcg cgcggcgttg atcaggcgct ccatctcaaa gttgtacatc
    20941 acattgagga aacccattcc ttcttcgccc accatgtcgc tctattcaac ctccacgttg
    21001 tcgagataga cttcgcaggt gctgagcata tgccagccga ttttatgcag cgggttaatc
    21061 ttaatgccgg gcttactgga gtcgacccac cacagggtga aggctttttt gggatctttc
    21121 ggttgcggat cgcgcgccag caccagcata tacgggtact ctttcgcacc ggtaataaag
    21181 gttttttgtc cgttgatgta gaccttgccg tttttacgcg tgtaagtggt agtggcactg
    21241 ttgttatctg agccagcacc tggttccgtc aacgccaggg cataggcggg atcacccgtt
    21301 tccagggtgc tctctgccgt tttacgtagc tgctctgcag aaccgaaacg gcgcatactg
    21361 tgaatacatt gaccgttggt aattaaaaat gccggagcac cgcattttga cacttccatc
    21421 agcgccagca tttgggtgac gtaatccgca gggataccac caaattcttc cggcacgcca
    21481 agcatggaaa taccgttatc cgccagcgcc cgcataaact cacgcggata tgtcccgttt
    21541 tgatcgcagg tgcggaaata ctcttccgga aagttagtcg taatcagttc gcgaatactg
    21601 gccagcagca gttcttgttc ttcagttaaa gaaaaatcca tcattatact cctgttaatg
    21661 agccgcttca ggcaggacaa aacccatcgc cgcatcgata aataatcttt cgtccatcag
    21721 tttgagttct ggcgaaatca ctggggtgaa gtccattttg tcgagaatat ctttttgtaa
    21781 atcgacgcca ggggcgattt cgattaaatg caggccgtct tctttcagcg tgaatacagc
    21841 acgttcggtg atgtaacgaa catccagtcc ccgttcgagc gcgatttttc cgctaaaagt
    21901 aatttccggt agttcccgaa taaatttatt tacccgtcct tcctggacga tatgcaattt
    21961 gccatcggca atttctgttt ttaaactgcc cgcagttaac gtgccgcaga aaataatttt
    22021 cttcgaggtg gcactgatat caataaatcc accggtaccc atgattttgc cattgaattt
    22081 atgcacgccg acgttaccgt gctggtcgac ttcagcaaaa ctcaaataac aaacatccag
    22141 accaccaccg tgataaaaat caaactggga cgtcatatcc agaatggcgc gggtattcac
    22201 gttcgcgcca aaggcgatac cctgtgaagt aattccgcca atcggacctg tttctaccgt
    22261 cagaataaag tcatcagcac aaccttcttc ccgggcgacc aggccaatac cgtcagcaat
    22321 accgacgccg acattcccca ccgcgccttt acgcatttcg aataatgcac gccgcgcaac
    22381 taatttacgt tggtttaggg gcaggctaag tttggtgctg tcatcaaggg tgaagtcacc
    22441 agaaataaaa cggttaaccg gcgcaccgcc atacagttgg ctttgatccg gatcgaccac
    22501 cacaatatcc accagataac ccggaatacg gacagattta ggatgcagcg tggctttctt
    22561 aaccattttc tgcacctgca tcatcacaat accgccgtta ttgtgcaccg cctgggcaat
    22621 aaccaatgcg tcgagataca tcacctcatc ttcaaaagtg gcgtagcctt cactgtcgca
    22681 ggtggtagcg cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt
    22741 gttatcaaac tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc
    22801 ttgctggcgt ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg
    22861 ggctgcggcg gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat
    22921 tttattttgt tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc
    22981 ccatttcacc agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag
    23041 ccctgtcgga ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc
    23101 aagagctgta attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag
    23161 tgttgcttcg tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc
    23221 attaatacga ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc
    23281 ctacgccaga tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt
    23341 aaatatattt tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc
    23401 tggtgtaaaa tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag
    23461 gcttttttta ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa
    23521 attacgccag tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc
    23581 atctccaggg tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca
    23641 atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg
    23701 gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg
    23761 gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata
    23821 tcaaccaggc cgctgtcgat ggctgcacga ttgagagcga tataagcctc ggtggaaatc
    23881 gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg
    23941 gtttcacgga gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag
    24001 tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat
    24061 ttcacgcggg cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgtacca
    24121 atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc
    24181 tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc
    24241 cataacgtga actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg
    24301 caaccagctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca
    24361 gtgaccagca gtcccggatg taacagactg atatcattaa ctaatgattc attctcaagg
    24421 ggtttcatac ccacttttgt gccattggtt aaaatgtcgg cggaagccag ggcttcagca
    24481 aaggcttgct gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc
    24541 cgctgcgcga aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt
    24601 tcttttaaac cttcaattgc cccctgcgcg ccaatcgccg ttgaggcacc tccggcccct
    24661 aacagcacca ttgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg
    24721 cccgtgccgt cggtgttata gccgcgcagg tagccatcat cattaacgat ggtgttgatg
    24781 gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg
    24841 ttcggcattg atataccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg
    24901 ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc cgctttttct
    24961 aaggctttat tctgcatttc aggcgataaa ctgtggcgga taggataggc catcagccca
    25021 atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg
    25081 tgtttaaccg ggtgctaaat ttacgctcgc caaaccgcac atcattctca ggaataataa
    25141 aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc agcgcgaaag
    25201 taaaatcgag aacaatgata tattgaaggc cgatgttcga cagataaccg gtaatcagcg
    25261 gaataacaaa gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc
    25321 tttttgggaa aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg
    25381 agaaaccgat aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa
    25441 tggctgctgt tattgttgcc agtgcagagt taaatacatt agcccagatg ggccggacca
    25501 tttttttcag tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag
    25561 agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca
    25621 tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa
    25681 caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg
    25741 cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca
    25801 gaatacccgg aataatcagc ccatagccgt accagatatt attgagcaac atatagctca
    25861 ccagcattgg gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg
    25921 aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg
    25981 agttagcgat acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg
    26041 gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac
    26101 caaacttatc ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac
    26161 cggaaattaa gtaggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgaca
    26221 tattttgggc aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa
    26281 gaacagccag gataaatggc gtgctgaaag ccttatattg agacattttc acagctcctt
    26341 gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata
    26401 ctgaagatga ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg
    26461 tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt
    26521 aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt
    26581 agcgagtgat gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa
    26641 ccgatggcag cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga
    26701 tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct
    26761 gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca
    26821 acacctccag cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg
    26881 ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat
    26941 gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa
    27001 cccacggtgt agatactgag tagtttaatc gacatagtgt atgacatgcc tgcaacaaat
    27061 tgtccgtact gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca
    27121 tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt
    27181 ttctttatga caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac
    27241 agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca
    27301 gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct
    27361 ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa
    27421 gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataggcgata
    27481 atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc
    27541 atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt
    27601 aatcgaccaa tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa
    27661 agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg
    27721 taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag
    27781 caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccgttcatta ttgttgtaac
    27841 aacgacaatt cctttttgtg gagaaattaa caaagccgct ccagatcgtg tgcatataat
    27901 tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt
    27961 tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgcc
//
LOCUS       DAHBHC010000068        27884 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHBHC010000068 DAHBHC010000000
VERSION     DAHBHC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.068"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1584)
                     /gene="lsrK"
                     /locus_tag="M2A35_003484"
     CDS             complement(<1..1584)
                     /gene="lsrK"
                     /locus_tag="M2A35_003484"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HCB7199734.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAP"
     gene            complement(1663..2616)
                     /gene="lsrR"
                     /locus_tag="M2A35_003485"
     CDS             complement(1663..2616)
                     /gene="lsrR"
                     /locus_tag="M2A35_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCB7199735.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2865..4400
                     /gene="lsrA"
                     /locus_tag="M2A35_003486"
     CDS             2865..4400
                     /gene="lsrA"
                     /locus_tag="M2A35_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCB7199736.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4394..5422
                     /gene="lsrC"
                     /locus_tag="M2A35_003487"
     CDS             4394..5422
                     /gene="lsrC"
                     /locus_tag="M2A35_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCB7199737.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5422..6414
                     /gene="lsrD"
                     /locus_tag="M2A35_003488"
     CDS             5422..6414
                     /gene="lsrD"
                     /locus_tag="M2A35_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCB7199738.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAIPAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6426..7448
                     /gene="lsrB"
                     /locus_tag="M2A35_003489"
     CDS             6426..7448
                     /gene="lsrB"
                     /locus_tag="M2A35_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HCB7199739.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7475..8350
                     /gene="lsrF"
                     /locus_tag="M2A35_003490"
     CDS             7475..8350
                     /gene="lsrF"
                     /locus_tag="M2A35_003490"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCB7199740.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8374..8664
                     /gene="lsrG"
                     /locus_tag="M2A35_003491"
     CDS             8374..8664
                     /gene="lsrG"
                     /locus_tag="M2A35_003491"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCB7199741.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8721..9479
                     /gene="tam"
                     /locus_tag="M2A35_003492"
     CDS             8721..9479
                     /gene="tam"
                     /locus_tag="M2A35_003492"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCB7199742.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9483..10397)
                     /locus_tag="M2A35_003493"
     CDS             complement(9483..10397)
                     /locus_tag="M2A35_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCB7199743.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10604..12055)
                     /gene="uxaB"
                     /locus_tag="M2A35_003494"
     CDS             complement(10604..12055)
                     /gene="uxaB"
                     /locus_tag="M2A35_003494"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCB7199744.1"
                     /translation="MKTLNRRDFPGAQYPGRIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12282..13692)
                     /locus_tag="M2A35_003495"
                     /pseudo
     CDS             complement(12282..13692)
                     /locus_tag="M2A35_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(13831..14190)
                     /locus_tag="M2A35_003496"
     CDS             complement(13831..14190)
                     /locus_tag="M2A35_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCB7199745.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14190..15116)
                     /gene="glsB"
                     /locus_tag="M2A35_003497"
     CDS             complement(14190..15116)
                     /gene="glsB"
                     /locus_tag="M2A35_003497"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCB7199746.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15180..16500)
                     /locus_tag="M2A35_003498"
                     /pseudo
     CDS             complement(15180..16500)
                     /locus_tag="M2A35_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
     gene            16601..17482
                     /locus_tag="M2A35_003499"
     CDS             16601..17482
                     /locus_tag="M2A35_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199747.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17559..18673
                     /gene="yneK"
                     /locus_tag="M2A35_003500"
                     /pseudo
     CDS             17559..18673
                     /gene="yneK"
                     /locus_tag="M2A35_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18823..20013
                     /gene="ydeA"
                     /locus_tag="M2A35_003501"
     CDS             18823..20013
                     /gene="ydeA"
                     /locus_tag="M2A35_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCB7199748.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20038..20703)
                     /gene="marC"
                     /locus_tag="M2A35_003502"
     CDS             complement(20038..20703)
                     /gene="marC"
                     /locus_tag="M2A35_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCB7199749.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20915..21349
                     /gene="marR"
                     /locus_tag="M2A35_003503"
     CDS             20915..21349
                     /gene="marR"
                     /locus_tag="M2A35_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCB7199750.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21369..21752
                     /gene="marA"
                     /locus_tag="M2A35_003504"
     CDS             21369..21752
                     /gene="marA"
                     /locus_tag="M2A35_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCB7199751.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21784..22002
                     /gene="marB"
                     /locus_tag="M2A35_003505"
     CDS             21784..22002
                     /gene="marB"
                     /locus_tag="M2A35_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCB7199752.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22059..23498)
                     /locus_tag="M2A35_003506"
     CDS             complement(22059..23498)
                     /locus_tag="M2A35_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCB7199753.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRLYWFTDVHTRG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23523..25196)
                     /locus_tag="M2A35_003507"
     CDS             complement(23523..25196)
                     /locus_tag="M2A35_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCB7199754.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYVSSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25252..25563)
                     /locus_tag="M2A35_003508"
     CDS             complement(25252..25563)
                     /locus_tag="M2A35_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCB7199755.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25591..26913)
                     /locus_tag="M2A35_003509"
     CDS             complement(25591..26913)
                     /locus_tag="M2A35_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCB7199756.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLQHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27028..27339)
                     /locus_tag="M2A35_003510"
     CDS             complement(27028..27339)
                     /locus_tag="M2A35_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCB7199757.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27538..>27884
                     /locus_tag="M2A35_003511"
     CDS             27538..>27884
                     /locus_tag="M2A35_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCB7199758.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTG"
BASE COUNT         7244 a         6930 c         6575 g         7135 t
ORIGIN      
        1 aggcgctttc cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata
       61 aactgcctgc cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt
      121 gtgcgtccgt tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc
      181 ggcaccgacg ccagctgcaa tggcacatcc taatgcggtg gcttctttga ctaccggaat
      241 attgacgggt aatcccgaga catcagcgag aatttgactc cataatttcc cttttgaacc
      301 tccgccagca aaaaccagcg aggtgggatg aatattcgag aaatcagcaa tttgctgcaa
      361 gttacacgct gatacaatcg ccgcattttc ttccagcgca cggaacaatg tcgctttgtt
      421 acatttatcc gggtcaatgg acaagttaat aaaggaaggc gcagcgtgat accaggtttt
      481 aaagcgcatt ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact
      541 ggccatctct tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc
      601 ttcggcacag aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga
      661 ttcagcttgt accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc
      721 tgtcaccggc gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt
      781 ttgtgccgga cgcacaacgc ctaacccaag gcaaccaagc tgcacgtcgc ctcctccaac
      841 gaccaccgga gtgcccgcct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc
      901 cagcaatgtg ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc
      961 cagcaatgca ggtttccagt cacgggtggt tagatcaaga agtcccgtgg tgccagcgtt
     1021 agagggatcc accgccagtt cgccgctgag catataggcc agccagtcgc tgatcatggt
     1081 gatggttgat gcctgacggt aaatatcgga acgatggtgc gccagccaaa gtaatctggg
     1141 gatggcactt aaagccagtg tttgtccggt cgcgcgataa acttcgtttt caaaggtatt
     1201 gttgtgcagt tctttaagtt cgctaacttc gcgtgccgct ctggcatcca cattggcgca
     1261 ggcccagatc ggggctcctt cattattata taaaacaatg ccttcacgca tcgaacatgc
     1321 cgaaacggca gcgatatact ccggggctat gccggcgttg tgcagcgcct ggcgcataca
     1381 ctcacacgcc agttgccagt ttttgttgag atcaaattcc atagaaccag gaacgtccgg
     1441 tactgccagg tgccgccact ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa
     1501 tatcacagcc cgaatacttc cggtacctgc atccagcgcc atcaggtaat actttgattc
     1561 tgaaggggta aagagtcgag ccattagatt atcctcggct tatgcgttct gtctgttcct
     1621 ctatacgttc tccatcattc ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc
     1681 cgctgctgtg tcctgatcgg taaccagtgc gttgatataa ccgcctttca ttgcagcggc
     1741 aattgcttcg gctttatttt ctccccctgc cacgccaacc cggacgggta tggtcttcag
     1801 cgagcttaaa ggtaagccaa tcagttcgtt atgtatttta atatccgtga caacgtcacc
     1861 ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcgac caatcattaa
     1921 ctgttcgccc tggctgatat aaccggagcg aatgatcgtc gcgtcgtcct gttgactgac
     1981 ggccccaatg ccgacaatcg ccacatcggc tgcttgcgcg gctaacagaa catctttgac
     2041 gcaattttca ttttttagcg tacgggcaat gtcagcggag gatgcccgca acggagccgg
     2101 aataatattc acactgcacg ccgcgttaag ctgcccgata cccgtcatat aagaaccgac
     2161 gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg
     2221 cagcgtattc atggttgcct cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa
     2281 actcatcaac atatgcgccg cgcctatccc cagtcgcccg ccgacatcag catccgcaag
     2341 cccagggatc acccggacat gttgcagcga aaactgacga cgtaattgag tttcatattc
     2401 cagacagcct tcaaagcgag aattaatctg tacgcgaata atgccggact gatgcccttt
     2461 ctccagcaag cgcgacactt tcaaacgggt caggccgaga cgatcgctga tctcgctctg
     2521 ggtcagcccg tcgtgatagt aaaaccacgc gatccgcgcg acctgttctt cttcacacat
     2581 tccctgttct gaaattgccg aatcgttgat tgtcataatt cattcttcac tttgaacata
     2641 tttaaatctt taatgcaatt gttcagttct tgcgcattta tatctgtgat ggcagccaca
     2701 gtttgactct acgagcatga acaaacgcaa ccgtgaaaat caaaatagca taaattgtga
     2761 tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa
     2821 atacatttgt tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa acgagtgata
     2881 cccgcgcgtt accgctactt tgcgcccgct cggtttataa acagtattca ggggtcaatg
     2941 tcctgaaagg catcgatttt acgttgcatc agggggaggt ccacgccctg ctcggcggca
     3001 atggtgccgg taaatcgacg ttaatgaaga ttattgccgg tattacccct gctgatagcg
     3061 gtacgctgga gattgggggc aacaactacg ccagattaac gccagttcat gctcatcagc
     3121 tgggcattta tctcgttccc caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa
     3181 acatcctgtt tgggatggca aaaaaacagc tctccatgca aaaaatgaag aacttgctgg
     3241 cggcgctggg ctgccagttt gatctgcata gtctggcagg atcgctggat gtcgccgatc
     3301 gccaaatggt ggaaatcctc cgcgggctaa tgcgcgactc gcggattctg atcctcgatg
     3361 aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc
     3421 tgcttgctac tggcgtgggt attgttttta tctcgcataa gctgccggaa attcgccaga
     3481 ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg
     3541 aactgtctac cgacgacatt attcaggcca tcaccccagc ggtacgggaa aaatcgctct
     3601 ctgccagcca aaaattatgg ctggagttac ctggtaaccg cccacaacat gctgccggaa
     3661 cgccggtgct gacactggaa aacctgaccg gcgaaggttt caggaatgtc agcctgacgc
     3721 tcaatgccgg agaaattctg ggcctggctg ggctggtggg ggccggacgc acagaactgg
     3781 ccgagacgct ctatggtctg cgtactttgc gtggcggacg cattatgctg aatggtaaag
     3841 agatcaataa attatccact ggagaacgtt tactgcgcgg tctggtttat ctgccggaag
     3901 atcgccagtc atccggactg aatctcgatg cttcgctggc ctggaacgtc tgcgccctta
     3961 ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga taatgccacc ctggaacgtt
     4021 atcgtcgggc gctgaatatt aaatttaacc aaccggaaca agctgcacgg acattatccg
     4081 gtggcaacca gcaaaaaatc ctcattgcca aatgcctgga agcttcgccg caagtattga
     4141 ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg taatgatatc taccagctgt
     4201 tgcgcagcat cgccgcacaa aatgtggcag tgctgcttat ctcctccgat ctggaagaga
     4261 tcgaactgat ggcagatcgc gtgtatgtga tgcatcaggg cgaaattgcc cactctgcac
     4321 tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc
     4381 gtcaggaggt gtcatgctga agtttattca gaacaaccgt gaaatcacgg cactgctggc
     4441 ggtggtgctg ctgtttgcat tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac
     4501 gctgaccatg gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct
     4561 ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg attaccggaa tgtgcgcagt
     4621 gctgttgggg atgttactga acgcggggta ttcactacct gttgcttgtg tcgcgacttt
     4681 actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg gtcgcgtggc taaaaatccc
     4741 tgccattgtt gccacccttg gcacgttagg gttgtacaga ggcatcatgt tgctgtggac
     4801 tggcggtaaa tggattgaag ggttacccgc cgaactgaaa cagctctccg caccgctgct
     4861 gtttggcgtt tccgcgattg gttggttgac gataattctg gtggcattta tggcctggct
     4921 gctggcaaag acggcgtttg gacgcagttt ttatgccacg ggcgataatt tacagggcgc
     4981 tcgtcaactg ggtgttcgta cggaagccat tcgcattgtg gcattttcgt tgaacggctg
     5041 catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac
     5101 cggtaccggg ctggagatga aagcaattgc agcctgcgtg ctgggcggca ttagtttgct
     5161 cggcggttcc ggtgcgatca tcggtgcggt actcggcgca tggttcctga cgcagatcga
     5221 tagcgtactg gtgctgttgc gcattccggc atggtggaac gattttatcg cgggcctcgt
     5281 gctgctggcg gtgctggtgt ttgatggacg cctgcgttgt gcgctggaac gtaatctacg
     5341 gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc gttaaacccg cttcgtcagg
     5401 taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca
     5461 ctgctcgtta ttgagattgt cgcatttggt gcaattaacc cgcgaatgtt agatctcaat
     5521 atgttgctgt tcagcaccag tgactttatc tgcattggca ttgtcgccct accgctgacg
     5581 atggtgattg tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc
     5641 attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat tctcctgacc
     5701 ttactgctcg gcgcattgtg cgggctgatc aacgccggat taattatcta taccaaagtt
     5761 aacccgctgg tgattacgct tggcacgctg tatctgtttg ccggaagcgc cctactgctt
     5821 tccgggatgg ccggagcgac ggggtacgaa ggtattggtg gattcccgat ggcgtttaca
     5881 gatttcgcta acctggatgt gctgggactc cccgttccgc tgattatctt cctgatatgt
     5941 ctcctcgttt tctggctctg gctgcataaa acccatgccg gacgtaatgt gtttttgatt
     6001 gggcaaagcc cgcgcgtggc gctttatagc gcgattccag ttaaccgcac cttatgtgcg
     6061 ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt
     6121 ggttcagcac gttccgatct cggtgcgtcg tttctgatgc ccgccatccc cgccgtggtg
     6181 cttggcggtg ccaatattta tggtggttcc ggttccatta tcggcaccgc cattgcggtt
     6241 ttattagtgg gatatttgca acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc
     6301 agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat
     6361 cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata acccattgcc ataaaggata
     6421 tcttcatgac acttcatcgc tttaagaaaa tcgccttact tagcgctctt ggcattgccg
     6481 caatctctat gaatgtgcag gccgcagagc gtattgcatt tattcccaaa ctggttggcg
     6541 tgggattttt taccagcggt ggcaacggcg cacaacaagc gggtaaagag ctgagcgttg
     6601 atgtgaccta cgacgggccg acagaaccca gtgtttctgg tcaggtacag ttgattaata
     6661 acttcgtcaa tcaaggttat aacgccatta tcgtttctgc ggtttcgcct gatggcttgt
     6721 gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt gctgacctgg gactctgata
     6781 ctaaaccgga gtgccgctct tactacatta atcagggaac gcccgcccag ttgggaggta
     6841 tgttggtgga tatggcggcg cgtcaggtga ataaagacaa agccaaagtc gcgtttttct
     6901 actcaagccc caccgttacg gaccaaaacc agtgggtgaa agaagcgaaa gcgaaaatcg
     6961 ccaaagagca tcctggctgg gaaattgtca ctacgcagtt tggctataac gatgccacta
     7021 aatcattaca aaccgcagaa ggaatattaa aagcgtatag cgatctcgac gccattatcg
     7081 cccccgacgc caacgccctg cccgctgccg cacaagccgc agaaaacctg aaaaatgaca
     7141 aagttgcgat tgtcggattc agtacgccaa acgtgatgcg tccatatgtg gaacgcggca
     7201 cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg caaaatttca gtgtatgtcg
     7261 cggatgcatt attgaaaaaa ggatcaatga aaacgggcga caagctggat atccagggcg
     7321 taggtcaggt ggaagtctcg ccaaacagcg ttcagggcta tgactacgaa gcggatggta
     7381 atggcatcgt actgttaccg gagcgcgtga tattcaacaa agagaatatc ggcaaatacg
     7441 atttctgatg tgcattactt aaccggagta agttatggca gatttagacg atattaaaga
     7501 tggtaaagat tttcgtaccg atcaaccgca acaaaatatc ccttttatcc tgaaaggttg
     7561 cggtgcgctg gattggggaa tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg
     7621 taaaaccgtg atgctggcct ttgaccatgg ttattttcag gggccgacta ccggacttga
     7681 acgcattgat ataaatatcg ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg
     7741 cggcattttg cgcagcgtag ttcccccagc gaccaataag ccggtggtac tgcgggcgtc
     7801 aggtgcgaac tctattctgg cggaattaag taatgaagcc gtggcgttat cgatggatga
     7861 tgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga
     7921 acatcagtcg atcaaaaata ttattcagct ggttgatgcc ggaatgaaag tgggaatgcc
     7981 gaccatggcc gtgactggcg tgggcaaaga tatggtgcgc gatcagcgtt atttctcgct
     8041 cgcgactcga atcgccgctg aaatgggggc gcaaattatc aaaacctatt atgtcgaaaa
     8101 aggttttgaa cggattgttg ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa
     8161 attaccggag cgcgaggcgc tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg
     8221 tgtggatatg gggcgtaata ttttccagtc tgaccatccg gtggcgatga tgaaagccgt
     8281 acaggcggtg gttcaccata acgaaacggc tgatcgggca tatgaactct atctgagtga
     8341 aaaacagtaa ctgcggattt aaggagaaga attatgcacg tcacactggt tgaaattaac
     8401 gttcatgaag acaagattga cgagtttatc gaagtttttc gccagaacca cctgggctct
     8461 gtacaggaag aaggcaattt gcgcttcgat gtcttacagg acccagaagt gaattcgcgc
     8521 ttttatatct acgaagccta taaagatgaa gacgcagtgg cattccataa aaccacgccc
     8581 cactacaaaa cctgtgtcgc gaaactggaa tctttaatga ccgggccgcg taaaaaacgt
     8641 ctgttcaatg ggttgatgcc gtgaggcgaa ttcatcagtt ttatctacaa ttggggtaac
     8701 gcgctgacgg gagtaaataa atgtctgact ggaacccctc tttatatctg cactttgccg
     8761 ctgaacgatc gcgtccggcg gtggagctgc ttgccagagt accgctggag aatatcgaat
     8821 acgttgccga tcttggctgt ggcccaggta acagcaccgc ccttctaaac caacgttggc
     8881 ctgctgccag gataacaggc atcgactcgt ctccggcgat gattgctgaa gcgcgcagtg
     8941 ctttgccaga ctgccagttc gtggaagcag acatccgcaa ctggcaaccg gaacaggcac
     9001 tcgatctgat ttttgctaat gcctcactgc aatggctgcc cgaccactac gaattgtttc
     9061 ctcatctggt ttctttactt aatccgcagg gcgtgctggc agtacagatg ccagataact
     9121 ggctggagcc gacccatgtg ctcatgcgcg aagttgcctg ggaacaaaac tacccggatc
     9181 gcgggcgtga gccgttggct ggcgttcatg cttactacga tattttgagc gaagccggat
     9241 gtgaggtcga tatctggcga accacctact atcaccagat gccgtcacac caggcgatta
     9301 tcgattgggt gactgccact ggattacgtc cgtggttaca ggatctgacc gagagcgaac
     9361 agcagctttt tcttaagcgc taccatcaga tgctggaaga gcagtatcca ctgcaagaga
     9421 acggacagat actgctggca tttccgcgtc tttttattgt tgcccggcgt atggagtaaa
     9481 tatcacgtca gctggtaatg acgatcggga agaatttttg ctggaatttc ggcttcatcg
     9541 ttcatctgta acaggtcaat ttcaatagcg ttgcagatgg catccagtgg taaatcattg
     9601 ttttcagtac cgaacggatc ttccagttct tccgccagac aatccagcga aataaaagtg
     9661 taggaaatca gcacagagat aaaaggcgtc atgtaatgca ggtccacgac cagcgcgaac
     9721 ggcagcatga tacaaaacag ataaacggta cgatgcaaaa tcagggtgta ggcaaaggga
     9781 attggcgtat aggcaatgcg ctcgcatcct gccaggaccg ctgaaatatc attaagccga
     9841 tcgttgaggc taataaacag gatatctgaa agctgcccat tgcggcgctg aaccgccaac
     9901 cactccccca ttattaacaa gatacggtta gctggagagt tcgaagccag tacaggctga
     9961 agatcttcag tcttgagata atgagccagc acttccgcct gtggctgttt gcgtaatgtc
    10021 atgcgtaaac agtgggcgaa ggcgatttgc aaccgggcaa actcccttac acttgccgaa
    10081 tccggcaatg tcgtttttac ctcgcgcagt aacgaccgtg aggcaatcat caactgcccc
    10141 caaagttttc gcgcttcaac gtaacgggcg tacccggcat tattacgaaa accaagaaaa
    10201 atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg tgaatttgat gcccagatgc
    10261 gtgtaccagg gcagcatgaa aataacagcg atagaaaaga gaaaattgag tagtaagcgc
    10321 gatgatatct tggataatac tgagccgtgc cagacaaaaa tacggcgcag ccagtgttgt
    10381 tgtggacgaa caatcatggt tatcttcagg cgtgaaaaag tcgccctatt aaacgtgatt
    10441 acgatcacat tctcaagacg ctatttacaa attacctaca agcttacaac attaacaggc
    10501 gctgcatgtg gcatctgtta atgataaagg cacataggat gttgtaacta atatagtgaa
    10561 ataagaaacc cggtcgaaac cgggttcaga agtaacggtg ttattagcac aacggacgta
    10621 cagcttcgcg catccctttt tcaagaatcg catccaggtc attagcaacc tgctcgacca
    10681 gaccaggcac ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac acaatcgcta
    10741 ccagttcctg ggtaccgata acacggtcac gatgctggct ccagagttgc tgataacgtt
    10801 ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc gttacgctca ccacgataga
    10861 acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg tttgccttct
    10921 gccctgccag cagctgcggc aggatgcggg tgcggaactt ggtcataccg ttgagcgcga
    10981 tagacagcag ctgatgctta atgtacgggt tacggaaacg cccggtgact gcactggcga
    11041 aagattccag ttcatcacga ggcaaatcca gtaccggaat aatttcttcg tagatagctt
    11101 tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta tccagccctg
    11161 cctggaacgc caccggcacc agcgcggtgt gcgcaccgtt gaggattgcc actttgcgtt
    11221 ctttgtacgg tttaatatcg tcaacaatca gcacgttgag cggatatttg tccagacgca
    11281 gttcagtcgc taaggatttc ggtccctgaa tcacaaacag gtaaaagtgt tcagcggtgt
    11341 cgagaaaacc atcgtgataa cccagctctt cttccagttt agccacttca tcgcgcggat
    11401 aaccggtaac gatacggtcc accagcgtag aacagaagct gttagcctga tccagccatt
    11461 gaataaatgc ttctggcagt gcccactctt gcgcatagcg cagcaccagt tcacgcaacg
    11521 cgtcgccatt gtagtcgatc aactcacacg ggatgatgat ccaaccttta tccagcgcac
    11581 cgttgaaatg gctaaaacgt tcgaacagca gacgggtcag ttttgccgga taacttaccg
    11641 ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat acccgcttcg gtggtattag
    11701 agaaaacaaa gcgcatttcc gggttgtgag ccagtttcag gaattcatcg tattcactgt
    11761 agacgctgat ttcacgatta accgagcgaa tcagacgcgc gtcgctgacc gcttccccct
    11821 tctcattcag gccacggata atggtggtat acagaccatc ctgcgtgctc agtgacggtg
    11881 ggaatgaagt ttcaatcgga cgaacaacga ccacaccaga attcagatcg gtgtgctcat
    11941 tcaggagatc gatttgccag tcaacaaagg cgcgcaggaa gttaccttca ccaaactgaa
    12001 tgatacgtcc tggatactgt gcaccgggaa aatcgcgacg atttagtgtt ttcacaatgg
    12061 gttcccttct gattagtcat acaacctgct tgaattggtg cgacaggtta gcaaacttta
    12121 atacgccgaa cccctgtttt gatcaactcc tgatgattaa tgagcagttt tatgagaaaa
    12181 gtgtggcgcg gatcatggtt taatcgagga aatacgcctt ttcctggatc ataaagtggt
    12241 agaacacatt gcattcaaat cgcgcggaat gaataaagat gtcagacaac ttcctcaccg
    12301 taacgcatag tgctggtacg gttgcgccca tctttcttcg agcgatacag acacgtatcg
    12361 gcttcgacca tcaatttatt aaaatcatcg gtcagcgttc ggtacgatgc gcgaccacta
    12421 ccgacgccaa tacttaccgt gagataaagc gttttttgtt gccaggtgaa tggttgcagt
    12481 tcaacgcctt tacggatttt ttccgccatt agcagaccat cgacaggatt caccgacggc
    12541 actgcaacag caaattcttc gccgcccatg cgcgccacca gccccttatc accgacaatc
    12601 ttctgaatat gccgggcaaa cacgcttaac actttatcgc cacattcatg tccatagtta
    12661 tcgttaatgc ttttgaaata gtcaatatca agcagcatga ccgtcagatg ttgtgtctgt
    12721 ttcagcgatg gacttttcag cgcctcataa agaccggacc gtgagtaaac ctgagtcaga
    12781 aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg aattgatcgc tgccacgcta
    12841 aaagaaacca taattgggca tatcgccatc gtggcaatac cgagacgtgc ggagaacatt
    12901 tccggaatgg agaacggcga accgaccgag atatcaatca ccgaatttgc caccagtacg
    12961 atttccaccg caccggtgac aaaggtcaac agacatgtta cctgcggcgt atagcgcact
    13021 gcacaccaga ttaatgcagg cagcgggaac gccagactcc ccgctccgcc aatgactacc
    13081 gaggcaataa ccgacacaat aagcgcgatg gcgggcatca tctgttctgc tttaaagcgc
    13141 tccaggaata gccaacgtca gcatacaagg cacgatcaac acgcccgttg agaattgctc
    13201 actgaaccag tccgcaagca aaggccagaa atccagacta tcaatactga ccgaaccaat
    13261 cgccccgaca atagcgcata ataatgcggc aatcagacag taattaaata gccgtaaggc
    13321 acttactggc tcatacttat ttttccccag acgcttatcc cgcacgacca gtaaggcgac
    13381 ggtaacaata aacatcatat tggagaaatt gataaccagt gaaaccagtc cccattcggt
    13441 ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat gcagtcgatt
    13501 aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc agaaaagaga
    13561 aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg ttagcgcaaa
    13621 aatagcgatg ctattacgta gcagatgacc ttcctggaac aaacggaaag ttgaaatggg
    13681 gtgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct caacgtaata
    13741 agtaaattca cataaaggat agcatgttcg ctaacagcgg cggcaaataa aggtaccctg
    13801 ggaatgctat tttatcgttt tccgcaattc tcatggctga ttcatctgta caaccagcca
    13861 gtgatagatt accgccacga tataacgttg ttgttcttcg cttaacgaaa caccctgcgg
    13921 aatgttatgc cacttagcca gacaaccgcg acagcaggtc gcggtggcgt gctgagcgat
    13981 aaacaccgga tgcccgcgca tgggggtttg cttaccgtca ttagccggta atgccggagc
    14041 aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg actggcgcgc ctttctccag
    14101 gcaatactga cgctctttca tgcccagacg aaagcgagag cgaaattttg aacgggataa
    14161 acgcgcgaag agtggatcga gagactgcat taataaaccg aacgccctaa ctgtttcgtc
    14221 aattgttcaa ggacggcgat acccgcaagc gagttacctg catcatccag ttccggactc
    14281 cagactgcga tggccatttc atgcggaaca atcgccacaa taccgccacc aacgccagat
    14341 ttcgccggta gtcccacccg ccaggcaaac tcccccgcgt tctggtacat accactggtc
    14401 gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc atcaatatga
    14461 atagctttcc cctgattagc cagaaagaca aacgtccggg ccagctctac acagctcatt
    14521 ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacgtcatg atggaaattg
    14581 ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc aaattcggaa
    14641 cgcgctacca ccgtatcgta tgaaatatca gacacaccgc ttaagccgcg cacgacttcc
    14701 agcatacgtt gccgtggtgc gcttagtcgc ccttgcaaca tatcgcagac caccagcgca
    14761 ccggcattaa tgaacggatt acgcggaata ccctgttcca tttccagttg cactaaagaa
    14821 ttgaacggtg atccagacgg atctttgccg acgcgttgcc agatttcctc ttcggagtaa
    14881 tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga aaaacgttct
    14941 tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat ccccaatcgg
    15001 gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg accaatgagc
    15061 ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac tgccactttc
    15121 tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa tatgtcgcgt
    15181 cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc gaaatgggaa
    15241 agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc gtcgctggca
    15301 caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg tctggcctgt
    15361 gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt agccagttcc
    15421 agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa cagggccaaa catttcttcc
    15481 cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata gtagttacct
    15541 gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag ggttttctcc
    15601 acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag agcgttctct
    15661 tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg ttcggtaaat
    15721 gcagaagcaa ttccctcttc gataataaag cgttttgccg ctgcacaaac ctgtccggta
    15781 ttctgataac gtccggctac cgccgcttta accgccagtt ccagatcggc atcgttaagc
    15841 acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc cgctccagcc
    15901 tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca ccgtgacagc agcaatgcgc
    15961 gaatctttaa tcatttgact gacaccgtcg ttgtcggcat tcagccagcc atatacgcct
    16021 tgcgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc catcacattc
    16081 ggcgcatgtt taagtaagta gccgttacct gcaagaatga tgggaaccgc gccacgcatc
    16141 acctgccata acggaaagtt ccacggcata atcgccagaa tcgtccccaa cggtcgatac
    16201 tcaataactg cctgctgatt ttccaccagc gtaggttccg ccttcagcat tgccggacca
    16261 tgttctgcat accagtcaca caaattcgcc gatttcgcta cgggcgcgca gagccttacc
    16321 gataccacgc agtttttcag cacgataatc tatatttgtc tcgcgccagt cgcgaaagcc
    16381 tgctgccgcc agctgaagtg cgttttcgat atcgttagcg ccagcccacg gcagcacaga
    16441 aagttgttca cccgtggcag gatttattga aattgcatga gttgccggag taatggtcat
    16501 cggggtatct cctttatgag tcatggtatg aagatacgca gatttactct tgctttaaaa
    16561 tgaataatat taagccactt attcacgaat cgagaatatt atggatctga cacaactgga
    16621 gatgttcaac gcggttgccg aagctggcag cataacccag gcagcagcaa tagtgcatcg
    16681 cgtgccgtcg aatctcacta cccgtctacg ccagctggaa acagaactgg gggtagatct
    16741 gtttattcgc gagaatcagc gtttacgtct ctctcctgcc gggcataact ttttacgcta
    16801 tagccaacaa attctcgcgt tagtggatga agcgcggagc gttgtcgctg gcgatgagcc
    16861 gcaaggttta ttttctcttg gttcgctgga aagcaccgca gcagtgcgca ttccggccac
    16921 gctggcggaa ttcaaccatc gttatcccaa aattcagttt tcactttcca ccggcccttc
    16981 cggcactatg ctggaaggag tgctggaggg aaaactgaat gcggcattta ttgatgggcc
    17041 cattaaccat actgccattg acgggatacc ggtataccgc gaggagctga tgatcgtcac
    17101 gccacaaggt catgcgccag taatccgcgc cagtcaggtt aacggcagta acatttatgc
    17161 cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc
    17221 cgctccggga actattcacg agatggagtc gtatcacggg atgttggcct gtgtggtcgc
    17281 aggagcaggc attgcgctta ttccacgctc tatgctggaa agtatgccgg ggcatcacca
    17341 ggttgaagcg tggccgttag ctgagcaatg gcgttggtta acaacctggc tggtctggcg
    17401 tcgcggtgcg aaaacgcgtc cgcttgaggc atttattcaa ctactggatg tgtctgactc
    17461 ggcaaaacag agttaccaat gagctatttt gatagttctt gcggctaata tgctctatat
    17521 agtgatgttc cgatgactta tgactatatg gggcaaatat ggttacgcca gtaagcatca
    17581 gcaattacat atctcttcct gatgattttc ccgttcgtaa tattgcacca caggtaaaag
    17641 aagttttaaa ggactttatt gatgcgctaa gtacaataat atgtgatgaa gagtggcgca
    17701 cgtctttaaa catcaactca gccacaaaaa agatatttaa taaccttgat aacttatcat
    17761 atattcagag gacttctttt cggggtaacg acacgctata caatgaaaag gttcagttta
    17821 aactcactta tcccgtcaag aatggaagac acaaagaaaa tattgaattt caggtagtaa
    17881 taaatttaag ccctatttat ttagataatt ttcgccatga tggagaaatt aatatttttg
    17941 cgctcccaac ccaaagcctg tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt
    18001 gctatttctg tttctgaatg attttattga acaatttcca atgttcaacc ttggcgttcc
    18061 catcaaaaga gcgcatacac cacatattga acccctgcct ccggatcgcc ataccgctgc
    18121 agattactta cgccagtttg atttgcttgt cctgaatttt atctctcgcg gtaattttgt
    18181 catactcccc cgattatgga ataactctga ggttcacaga tggttcgtca ataaagatcc
    18241 taacttgatc accgccattc tcgacataac ggacagcgaa ttaaaagagg atttattgca
    18301 aagcctgatg gattcattag gttctaacaa acatgtacta cccgaagtct gcatctgctt
    18361 tttatccctt ttagcagaac aagaatctcc tcattttcag gacttgtttt tatttttcgc
    18421 caatatgtta ttgcactatc accaatttat gaatcccaat gaaagtgatt tgaatgacgt
    18481 gttgatgcca gcatcattaa gtgatgataa aattatcaaa catatggcgc gcaggaccct
    18541 caaactgttt gtaaaaaatg aaacgccgcc aaaagttact cacgaagacc tggtgaaaaa
    18601 caggcctcgc tcccctgtca gaccacctat acccgcaacc gccaaaacgc cagacctccc
    18661 tgaacgtcat taaaccgtga tgttaccgac tctctgacgc ggaacagaat cagcgtcaga
    18721 gaaacggaaa acgcgatcca gatcacaaat gcattgtatt cacatcatta accgttttaa
    18781 gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac aaacactgtt
    18841 tcccgcaaag tggcgtggct acgggtcgtt acgctggcag tcgccgcctt catcttcaac
    18901 accaccgaat ttgtccctgt tggcctgctc tctgacattg cgcaaagttt tcacatgcaa
    18961 accgctcagg tcggcattat gttgaccatt tatgcatggg tagtggcgct aatgtcattg
    19021 ccttttatgt taatgaccag ccaggttgaa cggcgcaaat tactgatctg cctgtttgtg
    19081 gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gctttaccgt tctggtgatc
    19141 agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc gtctctggcg
    19201 atccgtatgg ctccggccgg gaagcgggca caggccttga gtttaattgc caccggtaca
    19261 gcactggcga tggtcttagg tttacctctc gggcgcattg tgggccagta tttcggttgg
    19321 cgaatgacct tcttcgcgat tggtattggg gcgcttatca cccttttgtg cctgattaag
    19381 ttacttccct tactgcccag tgagcattcc ggttcattga aaagcctccc gctattgttc
    19441 cgccgcccgg cattgatgag catttatttg ttaactgtgg tggttgtcac cgcccattac
    19501 acggcataca gctatatcga gccttttgtg caaaacattg cgggattcag cgccaacttt
    19561 gccacggcat tactgttatt actcggtggt tcgggcatta ttggcagcgt gattttcggt
    19621 aaactgggta atcagtatgc gtctgcgttg gtgagtacgg cgattgcgct gttgctggtg
    19681 tgcctggcat tgctgttacc tgcggcgaac agtgaaatac acctcggggt gctgagtatt
    19741 ttctggggga tcgcgatgat gatcatcggg cttggtatgc aggttaaagt gctggcgctg
    19801 gcaccagatg ctaccgacgt cgcgatggcg ctattctccg gcatatttaa tattggaatc
    19861 ggggcgggtg cgttggtagg taatcaggtg agtttgcact ggtcaatgtc gatgattggt
    19921 tatgtgggcg cggtgcctgc ttttgccgcg ttaatttggt caatcattat atttcgccgc
    19981 tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccattcgg gcctttttta
    20041 atggtacgtt ttaatgattt ccaggatgcc gttaataata aactgcacac ccatacatac
    20101 cagcaggaat cccatcagac gggagatcgc ttcaatgcca cccttgccca ccagccgcat
    20161 aattgcgccg gagctgcgta ggcttcccca caaaataacc gccaccagga aaaagatcag
    20221 cggcggcgca accatcagta cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc
    20281 cgagctaata atcatcgcta tggttcccgg accagcggta cttggcattg ctagcggcac
    20341 aaaggcaata ttggcactgg gttcatcttc cagctcttcc gacttgcttt tcgcctccgg
    20401 tgaatcaatc gctttctgtt gcggaaagag catccgaaaa ccgataaacg cgacgattaa
    20461 gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca ccagttgccc
    20521 ggcgtaatac gccaccatca tgatggcaaa tacgtacacc gaggccatca acgactgacg
    20581 attacgttcg gcactgttca tgttgcctgc caggccaaga aataacgcga cagttgttaa
    20641 tgggttagct aacggcagta acaccaccag ccccaggcca attgctttaa acaaatctaa
    20701 cattggtggt tgttatcctg tgtatctggg ttatcagcga aaagtataag gggtaaacaa
    20761 ggataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt gaaccgattt
    20821 agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctgg gcaatattat
    20881 cccctgcaac taattacttg ccagggcaac taatgtgaaa agtaccagcg atctgttcaa
    20941 tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag atcgtctgct
    21001 taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc tctgctctat
    21061 ccgctgcgcg gcgtgtatta ctccggttga actgaaaaaa gtgttgtcgg tcgacctggg
    21121 agcactcacc cgtatgctgg atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa
    21181 cccgaatgat aagcgcggcg tactggtaaa acttaccacc agcggcgcgg caatatgcga
    21241 acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa acctgacggc
    21301 ggacgaagtg gcaacacttg agcatttgct taagaaagtc ctgccgtaaa caaaaaagag
    21361 gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt ttggactgga
    21421 tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt tcaggttact
    21481 ctaaatggca cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta ggccaataca
    21541 tccgcagccg taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac
    21601 tctatctggc agaacgatat ggcttcgagt cgcaacaaac tctgacccga accttcaaaa
    21661 attactttga tgttccgccg cataaatacc ggatgaccaa tatgcagggc gaatcgcgct
    21721 ttttacatcc attaaatcat tacaacagct agttgataac gtgacaacgt cactgaggca
    21781 atcatgaaac cactttcatc cgcaatagca gctgcgctta ttctcttttc cgcgcagggc
    21841 gttgcggaac aaaccacgca gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc
    21901 ccatcgcagg aacaaccacc gtttgattta aatcacatgg gtactggcag tgataagtcg
    21961 gatgcgctcg gcgtgcccta ttataatcaa cacgctatgt agttattgtc aggctctgat
    22021 cacagagcct gacatctccg taatatcttc ctcaagtctc aaatattttc gccattgttg
    22081 gcgatgagat cccgatacca gtaaaacgac gctttacgta tccgttccag cgttcccttc
    22141 ccttcgttgt ctttatcgac ataaatcatt ccgtagcgct ttttcatttc ccctgttccg
    22201 gcagaaacca ggtcaatgca gccccacggg gtgtagccaa ttaagtcaac accatcttca
    22261 actacggctt ttttcatttc ccgaatatgg gaagcaaagt aatcaatgcg atagtgatcg
    22321 ttcaccgtgc cgtcagcttg tctctggtca accgcaccaa atccattttc gacaataaac
    22381 agcggcaact ggaaatgatc ccagaaccag ttgagtgaat aacgtagccc tgccggatca
    22441 atttgccatc cccattcgga tgtatcgata taagggttgc tgaccaaatc tcgtggttca
    22501 acataatcca gttgcgggtt atcgggcgaa aattgtgtcg taaaagacat gtagtagcta
    22561 aagccgataa agtcgacaca accactggca agaatcgcgt tatcttctgg tgtgatatcg
    22621 aggttgaatc ctttcctggc aaagtaattc agcatatgtt gcggataata tccacgagta
    22681 tgaacatcag taaaccagta aagacgatgc attgctttcg tggccatcat catatcgttg
    22741 ggtgcacacg tcagaggata gatgggacac atggcgatca tacagccaat gttaaattca
    22801 ggattgatcg attttccagt ctgtaccgcc agggcactgg caactaactc gtaatgtacc
    22861 gcctggtaca ttatttgttc gcgctcattg atatcttcct ctggcgaata caagataccg
    22921 gaattagtaa atggacacag gctttcgctg aaattcacct gattattgat ttcgttaaac
    22981 gtcatccagt actttacttt ttctttatag cgcgtgaaga ccgttgatgc gaagcggata
    23041 aaaaagtcga tcagtttacg gtttcgccag ccaccatatt ttgtcaccag atgataaggc
    23101 atctcaaaat gtgaaagcgt caccacaggt tccattccct gcttaaggca ttcatcgaac
    23161 agatcatcat aaaattgtaa accctcttca ttcggctcct gttcgtcacc ttgcggaaag
    23221 attcgtgtcc aggcaatgga agttcgaaag catttgaatc ccatctcggc aaataactga
    23281 atatctgttt tatagcgatg ataaaaatca attgcttcat gattgggata attaagcccg
    23341 tcgataacgc cttctgtcac ttcacgcggc accccgtgag cgccagcagt catcacatca
    23401 gcgatgctga tgccttttcc tccttcattc cagccacctt ccaactgatg cgcggctacc
    23461 gcgccgcccc ataaaaaacc ttttttaaat cctgacatac acaactcctt aactaaaata
    23521 gattaccacc agacttccgc ctggatgccg gccataaact cgtctttgct gttctcatta
    23581 aacttaaaat tcgataattc gttatccaga atattgatat acgttccata gaagcgaatt
    23641 tctggtcgtg aacctaaaat gctttcaccc actttcaatg catgccagag tgtcgtttta
    23701 taagccgatt catttagtgt tacaccctgc tgagttttgt tctgttgctt aaaccagcct
    23761 aattcaagcc ccgtctgatt ccatgtattc cagatccagg ccggtcttat tacagtgcga
    23821 atactgtcaa aatcactatg agcaccactt tcataactat aaacatcttc gccgtgagaa
    23881 tagacaagcg cgttagccat aataatattg tcagtaagat acatctcgcc ctgagagatt
    23941 aaacgccagg cgatcccatt ggtatgatca ccatagtaat agcgaccatg cgccatcgta
    24001 ttactggcat ctgagaaact ggcaaaactg ctgacatagg aattattggc aacctgtaac
    24061 gtaaattcat taaacgtatc gcgttgcagg ttttgccgta aaacagaagt cagcatccag
    24121 ctatctttca tttcaaaata actgttgtca ttttcgttat cttgttgttg atccgtttta
    24181 ttaggtgcgg aatatttagc cattaatgat aatgtcgcat catcccataa cgggatattg
    24241 cgataacgaa catccacaga attggtattc atctggctgg tacgcgaaaa atcacgagag
    24301 taaacatcga catcatcacg gcttaaggac atatcaaaca gccctacacc aagtgcccag
    24361 ttttcaatcc ccacacccgc ggcgacatcc gtggttaagg ttttccagtc cagcatctgg
    24421 atctcatatt gcgggagttt atgtttgccg acccagaagt ctgcctctgg tgcgaagggt
    24481 aaaaaacctc gcgttgtcag gtaaatatcg ctgaactgca tgatattttc attggcactg
    24541 tcaccaaacc aggcatcgtt atactgctca cccacgttgc catcataggt aacgaccgca
    24601 tttgccgttt taccgtcctg attataaaca cgctgattta aggtcaggtc aaaccaaccg
    24661 ctcatctcat taccaaaacg tcccagagaa cccgctgcat aagtttgtgg tgagccgtga
    24721 ttaccagtac cccagcctga gcggaagtac ccctgatagc tgaacccaat atcatccttt
    24781 atatatttac tgatatcttt cagggtcacg ctttcgatag tggttttgtc gcctttactt
    24841 tcaataacgg caacttgctg cgatccattt atagtatgcg ctgcggtaaa cgagttctgt
    24901 tcaccgtttt catttttgat gttatcagcg acgggagatg tcgctgatat ttcagcaagc
    24961 gaggctgatt tatatttgtt ttccgtgatg cttttttgta aggtcgaaag tcgggattta
    25021 tatactccca gttcattctg tgttgctttc agctcttgtt tattttgcga caattcattt
    25081 tcaagcaatt caaggcgctg ctctatggtt aattttgttg ccatcgcgtt caatggaaac
    25141 aatgctgtaa taatagaaaa agacaaaagg ctgacgttaa gcgtctttat attcatgact
    25201 tatccttaag agtataataa tttcctccag ctatcgcact ggaggaaaaa atcaggcatg
    25261 gagttctttt cttaatgaaa taatttccct ggctaaatcc tggcataata ttgcattcat
    25321 caaatgatcc tgtgcatgaa tcagaattaa attaactttt attttgccac agccttcatc
    25381 ctgactaatt aatgcggtct gaatcttatg tgcctgaaga caggcagatt cagaactggc
    25441 cattagctga gttgcgccct gccagtcttt ttgcccggca caacgtatgg cttccatcgc
    25501 gtcagaacgt gcctgccccg cgttgaccag taactccata acaagttctt catctgcaaa
    25561 catcacaatc tccttcctgg gtaaatggaa ttatccgtta gttgtcatgt tattgacttt
    25621 gttggcggca ataacaaaag gtaaatagat cattgtcgcc acacctaaac ataaaaaacc
    25681 aaccagtaaa gccagccagt tccccccggt tccgaggaaa gcaattaaag gtccgggcgt
    25741 agtccaaggc acggcataag caaccggtgg aataatttcc atcgaaacaa agaagtaacc
    25801 aatggcacag ttaacaatgg gaaccataat aaacggcacc atcatgatgg ggttaagtac
    25861 aataggaagg ccaaacatta tcggttcatt gatattgaaa ataccgggga taaatgacat
    25921 ttttgccaga tcacggtatt ccgcacgctt agaagcgata aaaatagcca acaataaccc
    25981 caacgtcata cctgaaccgc cgcagttggc gaatgcatca ttaatagaag tccaggtaat
    26041 cggatatggc gcgccccagg tagtgccatg ttgagcggcc caggagagat tctccagatt
    26101 agcctcagaa aaaacggttt ctcgaattgc cgatgtggta ttaggtccgt ggatccccag
    26161 cacccacagg aaattaccca cagcccccag gataattacc gcaaagatat tcgttcccat
    26221 atgtttgagc ggcgtctgga ttaatgtgta aataagatcg tttagtcctg ccggggatat
    26281 cagtgtcagg caataattaa gtgcggaaaa gaacaccatg acaaaaaata ttggcaataa
    26341 aactttaaat gaacgcgcta ccgcaggcgg tacagctgcc ggcatcgtta tggtgatttt
    26401 tgggtttcga gctaagcgac aaaatatttc actggtgatc aatgcaacga taacagcaac
    26461 aaacaacccc tgcgcgccga gatatttggt cgtcaggaat gcttgcccat ctatcatctg
    26521 atatggcgta taaacaataa aaaatgctcc tattgccgtt aatccgcata aaagatcatc
    26581 ttgctcatag ctaatcgcca tattcctggc gaccaggaaa gcaatcataa ttgacatgat
    26641 atttacagaa ccattcatta ccggagtaaa aatagcttgt gctttttcaa ggttggggaa
    26701 aatgctattc agatgctgta atccggcaat aaaaccgtct ggcgataata tggcaaagtt
    26761 aattaatata attaatgaac ttgccatgat aattggaaac gtaaaaacga atccatctct
    26821 caccgctgca acatgttttt gtgagtttaa cttgatagca actggaacaa gaaaacgttc
    26881 cattccacgt tcgaatgagg ccattaatcc cattttttta tacctgttgt tttggatatt
    26941 tatacatccc aataaagcat gtatgccata tccattttat gatgtgtgaa aagttagttc
    27001 aggtaatagt ttaataatat tagattctta ctcttgcagt agtttcatcg caaattcaag
    27061 aactgcctgt ccgttcatgg tgccgtagtc tttcatctcg ataacggcta caggcatttt
    27121 cccctgtgca acggcttcaa tctctggttt ctgaaagcga acttgcgggc caagtaaaac
    27181 aacgtcaact tcgttatttt taattttccc tttagcctct gcaatcgcca atgcggaaat
    27241 attaacttca agtgaaatag cgttagcatg atcaatcata cgtttaacca gcatactggt
    27301 tgacatgccc gcagcgcaaa ctaacaatat ttttttcata ttttccctta ctggtatata
    27361 acagattaca ttgtttaaat atcatatccc gcagtagcta tacgtcctgg tacattgatc
    27421 acagtaatac agtgataaag ttgttcaaca tcagggtgat tattaaacga ctcggccata
    27481 cgataaacca atcagaactc aactccttgt tttcaatggc tcttaaaagg tgcaaaagtg
    27541 atttttcaaa agattgcccg cttacttaaa tccgaaatca atggcaattc atggcatgtt
    27601 ggtgatttgt tgccgtcaga agcggaactc gctgttcgtt ataatgtttc gcgtaatacc
    27661 ctgcgtaagg cattatcctt gctggaaggc gaaggaatta ttcacagaaa gcatggttca
    27721 ggaacataca ttcaaaaaaa gaattttgtt gcacacattg atcacatgaa cagtttcagt
    27781 gaaattgcgc ataaaagcgg caaagaggca gggagtcaga ttatgaaatt tgaagtgcag
    27841 gatgcttcac ccactattgc gactgagctg aatttagtga ctgg
//
LOCUS       DAHBHC010000069        27624 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHBHC010000069 DAHBHC010000000
VERSION     DAHBHC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.069"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(26..898)
                     /locus_tag="M2A35_003512"
     CDS             complement(26..898)
                     /locus_tag="M2A35_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCB7199759.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(910..2004)
                     /locus_tag="M2A35_003513"
     CDS             complement(910..2004)
                     /locus_tag="M2A35_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCB7199760.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2037..3035)
                     /locus_tag="M2A35_003514"
     CDS             complement(2037..3035)
                     /locus_tag="M2A35_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7199761.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3060..4571)
                     /locus_tag="M2A35_003515"
     CDS             complement(3060..4571)
                     /locus_tag="M2A35_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCB7199762.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(4594..5577)
                     /locus_tag="M2A35_003516"
     CDS             complement(4594..5577)
                     /locus_tag="M2A35_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCB7199763.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(5674..8955)
                     /locus_tag="M2A35_003517"
     CDS             complement(5674..8955)
                     /locus_tag="M2A35_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCB7199764.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            9073..10266
                     /locus_tag="M2A35_003518"
     CDS             9073..10266
                     /locus_tag="M2A35_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCB7199765.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(10330..11583)
                     /gene="glyA"
                     /locus_tag="M2A35_003519"
     CDS             complement(10330..11583)
                     /gene="glyA"
                     /locus_tag="M2A35_003519"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCB7199766.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            11911..13101
                     /gene="hmpA"
                     /locus_tag="M2A35_003520"
     CDS             11911..13101
                     /gene="hmpA"
                     /locus_tag="M2A35_003520"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCB7199767.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13146..13484)
                     /gene="glnB"
                     /locus_tag="M2A35_003521"
     CDS             complement(13146..13484)
                     /gene="glnB"
                     /locus_tag="M2A35_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCB7199768.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13545..14879)
                     /gene="glrR"
                     /locus_tag="M2A35_003522"
     CDS             complement(13545..14879)
                     /gene="glrR"
                     /locus_tag="M2A35_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCB7199769.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(14869..15582)
                     /gene="qseG"
                     /locus_tag="M2A35_003523"
     CDS             complement(14869..15582)
                     /gene="qseG"
                     /locus_tag="M2A35_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCB7199770.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWHDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(15747..17174)
                     /gene="qseE"
                     /locus_tag="M2A35_003524"
     CDS             complement(15747..17174)
                     /gene="qseE"
                     /locus_tag="M2A35_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCB7199771.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(17749..21636)
                     /gene="purL"
                     /locus_tag="M2A35_003525"
                     /gene_synonym="purI"
     CDS             complement(17749..21636)
                     /gene="purL"
                     /locus_tag="M2A35_003525"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCB7199772.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNLLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTN
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            21894..23450
                     /gene="mltF"
                     /locus_tag="M2A35_003526"
     CDS             21894..23450
                     /gene="mltF"
                     /locus_tag="M2A35_003526"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCB7199773.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(23447..23983)
                     /gene="tadA"
                     /locus_tag="M2A35_003527"
     CDS             complement(23447..23983)
                     /gene="tadA"
                     /locus_tag="M2A35_003527"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCB7199774.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIEAQKKAQSSTD"
     gene            complement(24008..24643)
                     /gene="yfhb"
                     /locus_tag="M2A35_003528"
     CDS             complement(24008..24643)
                     /gene="yfhb"
                     /locus_tag="M2A35_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCB7199775.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            24852..25700
                     /locus_tag="M2A35_003529"
     CDS             24852..25700
                     /locus_tag="M2A35_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCB7199776.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRQSEA
                     LMKKLV"
     gene            26578..27423
                     /locus_tag="M2A35_003530"
     CDS             26578..27423
                     /locus_tag="M2A35_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199777.1"
                     /translation="MNKNPWDIPAWHALGREASLVTQLIGSGATAIGKANYADKIGEY
                     YTAFFGLSVGFERLAKLILVAHYAIEHKGKMPDENIVRKFGHKLIELTNEVDSISKKM
                     QLSLRYSRPTHKITYKILECLDSFADARRGRYANFSSLSSPNLTNNEPINKWWGEVAE
                     SILEKHYYYTVEQRRVEENARFINATLSPYTTVMYMNESTNIMQDLISASIRTGQNTI
                     VQKWGRFYSLSIARWLATVLAELSDIASHKYGIISFYGLSEHCCSYIVEDSFLKNRKI
                     WPLRQ"
     gene            complement(27476..27624)
                     /locus_tag="M2A35_003531"
                     /pseudo
     CDS             complement(27476..27624)
                     /locus_tag="M2A35_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         6759 a         7515 c         6857 g         6493 t
ORIGIN      
        1 aacgccttat ccggcatttt ataaattaca gcaacgcaac ccgcaacgac atctctgaag
       61 ttgttgattc acctggtgca agcgcaatga gatcaccccc ttccgggcga tgatgatcat
      121 ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg
      181 cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg
      241 cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt
      301 tcacccactg gcgcggcaac ggcgcgggct ggttaaaatc cagttcctgc ggtagctgct
      361 cgcaaaactc acccgccagc cactgctccc gctccagcca gtaaccgctc gcctgtgcct
      421 gaatccgcgt ttgcggcgac aacggaaaat aaggatgcca gccggtacca aatggcagcg
      481 tctctgcccc ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa
      541 acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac
      601 tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt
      661 gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacgat
      721 tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac
      781 cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc
      841 cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc
      901 gctaactcct taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag
      961 cgcataggca tcacctgcct gttccagcga gaagcgatgg gtaatggcgt tacgcggcca
     1021 cagcttccag tccgtcagat catgggcgca tttttccata tggaacagac tggtcaccca
     1081 ggagccgata atccgccgtt gatggtgcat cagatcggcg ctgacctcga attccacttt
     1141 tccggtttca ccaatgtaaa ccacccgtcc ccagtcagcg gtggattgca gtgccagcaa
     1201 gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtgagttc
     1261 ggcgataatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcacccctaa
     1321 ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc
     1381 gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac
     1441 gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca
     1501 actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc
     1561 ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc
     1621 tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga
     1681 aatgtgatac accagcacgc gatcaccctc tttaaaatgg cgacagcctt gccccatcgc
     1741 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
     1801 tgccgccgcc gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc
     1861 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
     1921 cagatcgacg gttgaatttc ctggtaaata agctgccagc atcgttttca taaatccctc
     1981 attaacaata cgattaattt tcatccctgc cgcacccgcg ccaggggcgt taaggtttag
     2041 cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
     2101 atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta
     2161 ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg
     2221 gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
     2281 gcacctgcgt taccagaacc gaggcgcgcc gccaacaaaa tgccggtcac cgccgctaat
     2341 aatcccgaaa gggtaaagat aagaatgcgc acccgacgaa cgttgatgcc gcacaactgc
     2401 gccgccgtag cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt tttgcggctg
     2461 atgaacacaa acagcgcaaa caacacaatc atgatcagcg cggataccgg cacaccgaga
     2521 aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttc
     2581 gtcataaaca gccccattcc gcgcagggcg ctccacagcc ccagtgtggc aacgaaactt
     2641 ggtacgttaa acacgccgcg cagcaccccg gccagcgttc ccatcagcgc gcctaacagc
     2701 aacaccagca gacacgccac cgccagcgga acttcaaatt gcagcaaaaa tgccaggcac
     2761 accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
     2821 agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg
     2881 ttattcaatg agataaagcc aggcgcattc agggagaaga ccagataaag aatggcgatt
     2941 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
     3001 ttgccctgcg gcaatgataa tgacgaagca gacatcttca ggttcctcgg taaaattcat
     3061 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc
     3121 tgcgagaacg taccgtgctg taacaacagg atgcggtcgc acaccagcgg caactcctcc
     3181 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg
     3241 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
     3301 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc
     3361 gtgccgatgg gcgtttcgct actggcggcc ttgaccgtca tccgctgcat cacctcttcg
     3421 gtcaggcggc ggatggtgga ccattgcagc actccgttgg cgctgatttt ttgccgattg
     3481 gtcagcactg tattttcgtc aacgcccaac cagggaatga tccccgcttc tttgcggttt
     3541 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg cgtgattttc
     3601 tcgccgttga taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc
     3661 aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc gcgacgtaac
     3721 gtaaagctga tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc
     3781 tgatccacaa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt
     3841 agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca
     3901 tcgcgcataa cggtggcaca ggaggcaatg cggcggattt cttccatccg gtggctgaca
     3961 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact
     4021 tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
     4081 atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt
     4141 tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc
     4201 aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc
     4261 gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca
     4321 cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
     4381 ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt
     4441 ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
     4501 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
     4561 gccgtaaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
     4621 cagccactgt ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt
     4681 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat
     4741 tttcttaccg gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc
     4801 aatttcggtt gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg
     4861 taccgctttt accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc
     4921 cggcgtggag ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc
     4981 ctgattagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt
     5041 acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
     5101 aattttattg gcaataaaat aatcggcggc agcgttaccc agttttttac caaattccag
     5161 tggatcgccg accagatacg ccgagacata tttatcgaca cccttttgat taatacaggt
     5221 gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac ggctactgcc
     5281 attttcagac actgccgata aaataatggc atcgacatta cgcgccacga gggtatcaac
     5341 aaaggtactt tctttcgaaa tatcaccctg ggcgttagtt tcaattaact gtacctgtac
     5401 tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat atccctgggt
     5461 atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa atagtgcgct
     5521 acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt taggcatagt
     5581 gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga tgagcggcag
     5641 cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa aaataaaagg
     5701 catcacggtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc
     5761 gcgagcagat tcatattgcg caacctcccc gagtcccgcg gacgccagca tcgccaccat
     5821 caggcatttt tctttatgct gctgttgtaa atcgccatac agcgacaaca ggtcaggttg
     5881 tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg cccactgttt
     5941 catttcacta aacagttgct gtgcgatttg ttgttcgccc agcagtcgca gcgccattcc
     6001 ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg
     6061 atcgccggtc gccgccagac gtaaacaacg cgtcgcttca gtttcatcgc cctgcgcgtt
     6121 ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta aacggccttc
     6181 gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct gctgcggctg
     6241 tctggcatca aggtgctgcc aggcgcgtaa taactggttg aggataaact gactggtgac
     6301 cttcccttcc ccgccttccc acgggtggaa tttgcgcgtg gcgagaatgt ccgccgcttt
     6361 gtctgcctga cccgtgagat gccacaaatt gagcaactct gctgtcatgt cgtcgcgttt
     6421 cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg tggctccgct
     6481 taacttatca agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata
     6541 agcattatca agataacgcg cggccagctc ataatcgtgt tgcttattcc acgcatggat
     6601 cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac gttgccataa
     6661 ggtaatggct ttgttgtagc tacgcttgtt gtagtagaag caggccagta aatgacgggc
     6721 aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg
     6781 gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccgcgtt cggctttcgg
     6841 caataaactc gcctgcaggt aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat
     6901 ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca tcagagcgtt
     6961 aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc tacgaccatc
     7021 gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca gtttcgcgcg
     7081 ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat gcaggcaaag
     7141 cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca gaccagcgtc
     7201 gaagttacca ttacgcgccg ccagtcgtgc caggccataa tagccaccgg ctttgctgtt
     7261 gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc
     7321 gtaagcactg gcgcgaatca aactggcctg tccgcactgc ggatttttgt tcagcgcatg
     7381 tgcgcgtttc agagcctgag tggcatacgc caccgcttgc gggaaatcgg cgcggttata
     7441 ttccagcatc gccagcgcca ggttacagcg ataatccagc ggatccagca ccacgccgcg
     7501 caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat gctgaccgat
     7561 aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcg agtggtgctt tggcgacgtc
     7621 cggcaacggc aactcttgcg gctgatgttc ctgataactc aggacaatat tgccgtcggc
     7681 atcggagagc tcaatggtca gcctttcagg attgataccg tgcaacacgc cctggatggc
     7741 ggtcgcaggc atcagtgcca cggcatcatc aagtaacgcg ttgcatttgc cgatttcgcg
     7801 gatcgccagg cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg
     7861 ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcccaaaga
     7921 atgataaggt aggaaatact gctcaaaacg cttctcttcg taagcatcaa gccaggtaaa
     7981 atcgggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattgtc
     8041 ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc actgtttttt
     8101 acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat cgtgacacca
     8161 cgcgccaaca aaatcgtact gtgatttttc agccatatat gaggttggaa cgggcacatt
     8221 tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc
     8281 gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgccgtta catccggcgg
     8341 gaaaacgctc tgatgccctt cccccccttt cactgccggg ttggcccacc acaagaaatg
     8401 acgcggcgtg gcgttaccgt tatagacgcg gctggcgatt tccagcgccg cccggtcagg
     8461 gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt cgcctaccca
     8521 caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca taaaggtggt
     8581 cgggcgatga tgttgcggcc agttaaactc aatcccaccg gaaatccacg gccccagcag
     8641 ccccaccagc gcaggtttaa tgacttcatt gtgataaaca aaatcgcgct gtttcacttt
     8701 atcccacgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc
     8761 gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca gcgtatcggt
     8821 cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat
     8881 gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac
     8941 ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg
     9001 gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa
     9061 ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc
     9121 gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg
     9181 gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag cgaaaaacgg
     9241 gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt
     9301 gcgccggaag gtgactggac gctgtgcctg aacgtaacgc ccaccagtat tgagtgtcag
     9361 gttgctaatg cttgtttaag tccgaaaggt gaatttgagt atttacagat tgatgcaccg
     9421 acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgtcatcg caaactgtgg
     9481 ccgggccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc ggtgaccggc
     9541 gtatcgcaaa ccatgccgca agcgccgtgg acaacgccgg ttgaggtgaa gtatctgctg
     9601 gaagagaagc tgggcattcg ggtgatggtc gataatgact gcgtgatgct ggcgctggcg
     9661 gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc
     9721 attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcgga
     9781 cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg acgttacggc
     9841 tgcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa
     9901 tcacaaccgg ttaatactca gcttgatcct gaaaaactga caaccgcgca gttaatcgct
     9961 gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc caatgccatt
    10021 ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt
    10081 cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac aggatttaac
    10141 ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt tgggcaatta
    10201 agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca gttacgacag
    10261 atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttttttc aacagattaa
    10321 ccgtttcgct tatgcgtaaa ccgggtaacg tgcgcagatg tcgagaactt tacctttgat
    10381 gcgctcgata acggcttcat cattgatgct gtccagcacg tcacacatcc agccagccag
    10441 ttctttcgct tctgcttctt tgaagccgcg acgcgtaatt gccggagtac ccacgcgaat
    10501 accggaggtc acaaacgggc tcttcggatc gttcggtacg ctgtttttgt tgacggtgat
    10561 gttagcacgg cccagagcgg cgtctgcttc tttaccggtc aggtttttat cgaccagatc
    10621 aaccaggaac agatggttat cagtaccgcc ggaaaccact ttgtagccgc gttccaggaa
    10681 cacttctacc atcgctttag cgtttttagc gacctgctgc tggtaagttt tgaactcagg
    10741 ctccatcgct tctttcagag caaccgcttt accggcgatt acgtgcatca acggaccgcc
    10801 ctgaccacca gggaaaacgg cagagttcag ttttttgtac agctcttcgc taccaccttt
    10861 cgccaggatc aggccgccgc gcggacccgc cagggttttg tgagtggtgg tagtaacaac
    10921 gtgagcatga ggaaccgggt tcgggtagac gccagcagca accaggcccg caacgtgcgc
    10981 catatcaacg aacaggtaag caccgatgct gtcagcgatt tcacgcattt tcgcccagtc
    11041 caccacgccg gaatatgcag agaaaccacc gataatcatt ttcggcttgt gttctttggc
    11101 ttgtttttcc agatcggcgt agtcgatatg accggtagca tcgataccgt aaggaacgat
    11161 gttgtacagt ttaccggaga agttaaccgg agaaccgtga gtcaggtgac cgccatgcgc
    11221 caggttcata cccagaacgg tatcacctgg ttccagcagc gcggtgtaga ccgcaaagtt
    11281 agcctgggag ccggagtgcg gctggacgtt agcgtagtca gcgccgaaca gttctttcgc
    11341 acgatcgatc gccagttgtt caacgatatc aacatactcg caaccgccgt agtagcgttt
    11401 gcccggataa ccttcagcat atttgttggt cagctgagaa ccctgcgcct gcattacgcg
    11461 cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc tcttcctgac gtactttttc
    11521 ctgctccata gcctgccaca gttcggcatc ataatcggca atgttcattt cacgctttaa
    11581 catccgcatc tcctgactca gctaacaata aaatttttgg cctttatagg cggtcctgtt
    11641 ggacaacggc gaacagtata accgaatcat tgtgcgataa caggtcttga caaaggaatt
    11701 tacgcaaacg attaccttca ggctacgcaa ggctttggag aataaagagc ttgcaaccgg
    11761 aaacggattt cttttcaggt ttgtgatgca aatttttcac ttcatcacat tctttctgaa
    11821 aaacaccaaa gaaccattta cattgcaggg ctatttttta taagatgcat ttgagataca
    11881 tcaattaaga tgcaaaaaaa ggaagaccat atgcttgacg ctcaaaccat cgctacagta
    11941 aaagccacca tccctttact ggtggaaacg gggccaaagt taaccgccca tttctacgac
    12001 cgtatgttta ctcataaccc agaactcaaa gaaattttta acatgagtaa ccagcgtaat
    12061 ggcgatcaac gtgaagccct gtttaacgct attgccgcct acgccagtaa tattgaaaac
    12121 ctgcctgcgc tgctgccagc ggtagaaaaa atcgcgcaga agcacaccag cttccagatc
    12181 aaaccggaac agtacaacat cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc
    12241 agcccggggc aggaagtgct ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta
    12301 tttatcaatc gcgaggcgga aatctataac gaaaacgcca gcaaagccgg tggttgggaa
    12361 ggtactcgcg atttccgcat tgtggctaaa acaccgcgca gcgcgcttat caccagcttc
    12421 gaactggagc cggtcgacgg tggcgcagtg gcagaatacc gtccggggca atatctcggc
    12481 gtctggctga agccggaagg tttcccgcat caggaaattc gtcagtactc tttgactcgc
    12541 aaaccggatg gcaaaggcta tcgtattgcg gtgaaacgcg aagagggtgg gcaggtatcc
    12601 aactggttgc acaatcacgc caatgttggc gatgtcgtga aactggtcgc tccggcaggt
    12661 gatttcttta tggctgtcgc agatgacaca ccagtgacgt taatctctgc cggtgttggt
    12721 caaacgccaa tgctggcaat gctcgacacg ctggcaaaag caggccacac agcacaagtg
    12781 aactggttcc atgcggcaga aaatggcgat gttcacgcct ttgccgatga agttaaggaa
    12841 ctggggcagt cactgccgcg ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc
    12901 gatcgcgcta aaggtcagtt tgatagcgaa ggtctgatgg atttgagcaa acaggaaggt
    12961 gcgttcagcg atccgacaat gcagttctat ctctgcggcc cggttggctt catgcagttt
    13021 gccgcgaaac agttagtgga tctgggcgtg aagcaggaaa acattcatta cgaatgcttt
    13081 ggcccgcata aggtgctgtg atttgatgtt gccggatgga aacatccggc aacccttgac
    13141 gcggtttaaa ttgccgcgtc gtcctcttca ccggtacgga tgcgaatgac ccgtgccacg
    13201 tcaaagacga agattttacc gtcaccgatt ttgccggttt gcgccgtgcg aataatggta
    13261 tcgacacagg tatcgacaat gtcgtccggt acgacaatct caattttcac tttcggcaga
    13321 aaatccacca tatactccgc gccgcggtac agctcggtat ggcctttctg gcgaccaaag
    13381 cctttcactt cggtcaccgt catgccggta ataccgactt cggccagtgc ttcgcggaca
    13441 tcgtccagct tgaagggttt tataatcgca tcaatctttt tcatgctatt ccttgaaaag
    13501 gtcgcctgtc ttttgatctg ctaaacgtaa cacataacgc caattcattc cttgaaatcg
    13561 tttgcatcca gctcgtgtcg ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc
    13621 attctcgccg cgtgggtgac gttgcctttg gtaatttgca gcagcttacg caaatagttg
    13681 agttcaaact gattacgtgc ctcaacaaag gttggcagcg ccgtgttttc accctccagc
    13741 gcctgctcta ctaatgcatc gctaataacc ggagatgagg tcagcgccac gcactgttca
    13801 atcacgttga ccaactggcg cacattaccc ggccagctag cggtcatcag gcgtttcatc
    13861 gcatcagtgg agaacgcgcg gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg
    13921 tgatttgcca gaagcggaat gtcttctgtg cgctctgcca gagccggaat tttcaggctg
    13981 acaacgttga ggcggtaata gagatcttcg cggaattccc cgcgcgccat cgcttttggc
    14041 agatcacggt gagtggcaga aataatccgc acgtcgatat caatatcgcg gttgctgccc
    14101 agcgggcgca ctttacgctc ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc
    14161 atatcgccaa tttcatcgag aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg
    14221 cgattgctga cagcgccagt aaacgcgcca cgcgcatgac caaacagctc cgactccagc
    14281 aattgctcgg gcaatgcgcc acagttaata gcaataaatg gtttgctgtt gcgcgggctg
    14341 gcgttgtgga tagcctgggc gaaaatctct ttcccggtgc cgctctgacc gttaatcaaa
    14401 acgctgacgt ctgattgcgc caccagccgc gcctgttcca gcaaacgcag catcagcggg
    14461 ctgcgggtga caattgcctc gcgccagcgt tcatcggtgg ctggcgcgga ttgctccagc
    14521 gcatcgtcaa ttgcctgata tagcgcgtct ttgtcgacag gcttggtgag gaaactaaaa
    14581 acgccctgct gtgttgcagc aacggcatcg gggatagaac catgcgcagt aagaataatt
    14641 actggcattc ccggctgcac tttctggatt tcagcaaata gctgcatacc gtccatttca
    14701 tccatccgca ggtcgctgat aactaaatct actttttcgc gattcagtac ccgtaatcct
    14761 tcagcgccac tttccgccgt gaccacactg tagccttcgc tggtcaggcg caggccaagc
    14821 agtttcagca atcccggatc gtcatcgacc aataataaat gcgcaggttt atggctcatc
    14881 aggagtgacc tcatgggtgg atggcgcggg cttttcactc tcgtgcggcg tatccggcga
    14941 gaaatttcca gccggtttgc gggtcgagag ctggcgttca atatcggtca ggttttccag
    15001 cttgcgggtg gtgagttcca gttgctgctg tagaacgtga tgttgctggc gcaatgtatc
    15061 cagctcgctg tcgctggact gctggagttt actgtaacgt tggcgctctt ccgccagttg
    15121 cagttgcagc gcctgaccgt cgtgccagag ttgatacagt gggcgaacct gtgccgggat
    15181 ctcggtactt aacgcttcaa tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt
    15241 ggcgtcggcg agcaaaatcc cttgtttaaa ggtattttgc cagctgccgt cgtcatattg
    15301 gcgagcttgc tgacgcgact gcgcaggcat taaacgatca gcacaatcca tcgcccgcag
    15361 ccagtaaagc ggattggttt cggttgattt accttgcagc gcccagatgt cgctacattc
    15421 agtagaaaga tagtcagcca gttgataaac cggaattttt tcttctgctg gcgtatcaat
    15481 ggctggctta ttgtgattct gcacgcaacc cagcaatgcc agacatggca gccctgccag
    15541 ccacagccgt cggggcaata atcgttgaaa aatgtgtcgc atattcacca gacttaaagc
    15601 ctatcccagt gggcgtaatt gttgcagaca gtctggacat ggacagcgcg gagaaaccgg
    15661 agcgtacata tcgtacgtga agatttcgag cactgcccgg ggccgaactg acaaataaaa
    15721 tagcctgata ggataggctg ttagcattat ttcgtgtttt tcgacgacgg taattcaatg
    15781 cggaaacaaa cgtcttgccc gctctcgtcg accagataca gttccccttg catacggcga
    15841 atacaatccc tggcaatgct taatcccaga ccgctgccct tcaccgcccc ttttcgctgg
    15901 tggcttccct gaaaaaaggg ttcgaagatc atggcgcgtt cctcttgcgg aattggcgtg
    15961 ccggtattga tgacatcaat ataaacccgc gcaccatgtg aactgctgcg aaggcaaatg
    16021 ttaccggatt cagccccgta gtgcaccgca ttggagtaaa gattatccag tacgctcatc
    16081 agcagcattg gctccgccag gcaagctgtt gctttgagat cgacgtcggt atgcatcatt
    16141 ttagcccgtg cgggcaggct atgagcagaa accactgtct ccaccagcgg tgctaattca
    16201 acattctcca gttccaccgc actgtccgcc tgtttacggt tgtaatcaag cagttgttcg
    16261 atcagttttt gcaaattgcg gctgctgcta tcaagaatgc tcaccacctc tttttgctct
    16321 ggcgtaagcg gcccgacaac ctggtcagcc agtaattcag tgccctcgcg catactcgcc
    16381 agtggcgttt ttaattcatg agataaatgt cttaaaaatt gatggcgttg ggattccagc
    16441 catgacaggc gctcacttaa ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt
    16501 ccactgaacg aaacgctatt gcccagagaa cgcccttccc ccagacggtt gatcatgcgc
    16561 tcgatatttt tcaccggccc gataatcatc cgcgtgaaaa gtagcaccat caccagactc
    16621 accagaaata gcaccagcga ttgccaacca aaatattgcc cacgttcggc gatttcacgc
    16681 tgaagttgct gcccacgaga gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg
    16741 gcactggcaa aggcttcaag acgcgcggcg gcggcagcat cgggaccact gttgttacac
    16801 tgaagttggg ccagattgtt caaatcctga cgcaatgcct ggtaaagttt atcgtctggc
    16861 agtacgcctg cgtgggcatc gagcatttcg ctgtaacgct tgcgctggct ttgataaacc
    16921 ttcgcaagcg taggatcgtc cagcacgcaa tactgacggt agctacgctc catctccagc
    16981 gccgcgttgg tcatcgcttc actacgccgg gcatcaataa gcgtagtgcg gttaaccagc
    17041 gccgcctgat cgctaagcgc attcaggctt tgccaggctt gccatgccag caccaacagg
    17101 ggtagtagaa ttagcaaaaa tgccagcatt accagttgtc gtaatgagcg gggaaaaaca
    17161 ggccagcgtt tcaaggtgtt actctcgtca gacgcgaata gcctgatgct aaccgagggg
    17221 aagttcagat acaacaaagc cgggaattac ccggctttgt tatggaataa ggcggtgcct
    17281 aactcgacgt ttcgcccgat ggttgatata gctacgctga tatcagaagt tggacggcag
    17341 gcaccttgtt gtgcgtcatt cgaattttat gtagcacgtc ccgaaggggc tgacataagt
    17401 cggtgaatga gccactgctt actatatgca gtaactgtgc caataaagaa aatagtttgg
    17461 taacgtactg attatacgtc gctttgaggg gtttatggct tctccgttat ttgaatgtaa
    17521 atcagccact gtgtcgctaa aaagagacaa cttaagataa acttattaga taatattaaa
    17581 atcaatgagt taagtgtcgc cagaaagcga cacggtaaac cacccattgt cgtgatttac
    17641 agacacaaaa aagctcccgg agttgggagc ttatgatagt ggttggtgct taatgccgga
    17701 tgcggcgtaa atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt accccaactg
    17761 cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttccggatg
    17821 ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg
    17881 accactttca gtcgtaatcg acgtaatacc gttcggtgaa ccgttcgggt tagccgggta
    17941 ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc
    18001 cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc
    18061 aatcggcatt tgcgagccca ccatcccctg caacagcagc gacgggcttt gggtcacttc
    18121 aaccaggctg aaacgcgctt caaagcgatc ggaggtattg cgcacaaaac gtggccacaa
    18181 ctcactacct gggatcagtt cgcgcaggtt agacatcatc tggcaaccgt tacatacccc
    18241 cagcgccagc gtttgcggac ggtggaagaa ggttgcaaac tcatcgcgta cacggtcatt
    18301 gaacaggatt gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc
    18361 accgcacgcg accagggcgt ggaaatcttc caggcccgtg cgtccagtca gcaggtcact
    18421 catatgcaca tcgattgcat cgaagcctgc acggtggaaa gctgccgcca tttcaacatg
    18481 cgagttcacg ccctgctcac gcagcacagc aactttcgga cgtgcgccag tggcaatata
    18541 cggtgctgcc acatcttcgt tgatatcgaa cgacagtttc acattcaggc ccggatcggc
    18601 gtcgtttgat ttcgcctggt gctcctggtc agcacactcc gggttgtcac gcaggcgctg
    18661 catctgccag gtagtttctg cccaccagac acgcaacgtg gtgcggcttt cgctgaatac
    18721 agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc ctacataatg
    18781 gacacaatca gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc
    18841 acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcgcc
    18901 cagagaggca atatccgcgt taatgccgca atgaccagca aaggccattt ccgccagcgt
    18961 taccagcagg ccgccatcag agcggtcgtg gtatgcgagc agcttacgct gtgcaaccag
    19021 cgcctgaatc gcgtcataga aacctttcag ttgcgcgaca tcgcgtacat ctgccggttt
    19081 gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt
    19141 gcccaaatca atcagcagca gtgcgttatc ttcggtagaa agctgcggcg tgatggtgtg
    19201 acgtacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc
    19261 gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg
    19321 gatcgtcagg cccagcgccg gacaaagctc ttcgcccacg gctttaacgg cttcatacag
    19381 gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg
    19441 tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg
    19501 ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc
    19561 accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca
    19621 cggacccacc atctgatcgc gcgctaccat gccagttacg ctgcggtcac cgatggtcac
    19681 caggaaagtt ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat
    19741 ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt
    19801 catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa
    19861 atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg
    19921 tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag
    19981 cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc
    20041 gcttagaata tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca ccagctccgg
    20101 catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata aacagaattg ggttagcatc
    20161 accaagctgc cagcaacggt cgatcacttc ctggcaacga cgctccatct ccgggttgtc
    20221 gcgctgtacg gaagcaaagt cgaggtcggc atcagactga cctgacgcca tggaagacgc
    20281 cgctccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc
    20341 gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag
    20401 catgatcggt ttgtgataac cgcgcagctc ttcgccgttg tggctgttca ctttttcttc
    20461 ataagtacgg aagtagccat tcagtgccgg acgaccaaat tcgttgttaa acgccgcgcc
    20521 gcccagcggg ccttcggtca tgatgtccag cgcggtgaca atgcgctcag gcttaccgaa
    20581 atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggaaacgg agaaaccaac
    20641 caaacccgct ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc
    20701 ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac
    20761 tttcatcaga atatgcgccg gttcctgatg gaaatcgtag cggcccgttt cgtggtcagc
    20821 aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag
    20881 aacgtaatct ggcgtggttt cgaaggtgtt tttgatcatc ttaaacagcg atttcggctg
    20941 ttgctcaccg tcgataaccc aatcggcgtt aaaaatcttg tggcggcagt gctcggagtt
    21001 cgcctgggcg aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc
    21061 atcctgcaga tagtcaattt catcttccgc cagtgccagg ccaagacgca ggttagcgtc
    21121 gattagcgcc tgacggccct gccccagcaa atcaacgttg gtgaccggag tcggttgatg
    21181 gtgtgcaaac agctgctctg catcatctaa agcaaaaaag accgtttcca tcatgcggtc
    21241 gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata
    21301 gtaagcaacg ccgcgctcaa ggaggtttac ctgttgtagc ccgcagttat gggcaatatc
    21361 ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt
    21421 gccttgcggg gcgtggctgg cgagtgccgg gccatatttc agcaggcgtt caagttgtgc
    21481 gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat
    21541 attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc
    21601 cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc
    21661 ggtgtaccca agggggggaa acgggcgtca ttataaagaa tctgatgcgc tgacgaaacc
    21721 gtttgcgtgg aaataaaatc accatcgtga attagcaacg cgtgccgcca atggctgtaa
    21781 taagttgcca tctggcgcag gtttacgcaa aatgccgctc atttatgagt aaacctttca
    21841 ctattattac gttttttcaa gctgggacgc gcacgacaca gagaattaac taattgaaaa
    21901 aattaaagat taattatctg ttcatcggca ttctggcact gctgctcgcg gtcgctctct
    21961 ggccatccat tccctggttt ggtaaagccg acaaccgtat cgccgccatt caagcgcggg
    22021 gagagttgcg tgtgagcacc attcatactc ccctgactta taacgaaatc aacgggaaac
    22081 cttttggcct ggattacgaa ctggcgaaac agtttgccga ttacctcggc gtaaaactga
    22141 aagtgaccgt gcggcagaat atcagccagc tgtttgacga ccttgataat ggtaacgccg
    22201 acctgctggc ggcaggactg gtctataaca gtgagcgggt aaaaaattat cagcctggcc
    22261 ctacctatta ttccgtgtca caacaactgg tttataaagt gggtcagtat cgcccacgta
    22321 cgctgggcaa cctgacggcg gagcaactca ccgttgcacc gggtcatgtg gtggttaacg
    22381 atctccagac cctgaaagaa acaaaattcc cggaattaag ctggaaggta gacgacaaaa
    22441 aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa actcgattac accattgctg
    22501 attctgtcgc catcagcctg tttcagcgcg ttcacccgga gctcgccgta gcgctcgata
    22561 tcaccgatga acaaccggtg acttggttta gcccgttaga tggcgataat accctttccg
    22621 ccgccctgct cgacttcttc aacgaaatga atgaagacgg tacgctggca cgcattgaag
    22681 agaaatacct ggggcatggc gatgattttg attacgtcga tacgcgcaca tttttacgcg
    22741 ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga gaaatacgcc gaagaaattg
    22801 actggcgttt gctggccgct attgcttatc aggaatcgca ctgggatgca caggccactt
    22861 caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa agcctcggca
    22921 ttacggatcg taccgatgcc gaacagagca tcagcggtgg cgtgcgttat ttgcaggata
    22981 tgatgagtaa agtgccggaa agtgtgccgg agaacgagcg gatctggttt gccctcgctg
    23041 cgtacaatat gggctatgcg catatgctgg atgcccgcgc cctgacggca aaaaccaaag
    23101 ggaatcctga cagttgggct gacgtaaaac agcgtctgcc tttacttagc cagaaaccct
    23161 attacagcaa gctgacttac ggctacgctc gtggacatga agcctacgct tatgtcgaaa
    23221 atattcgtaa gtatcagatt agcctggtgg gttatctgca agagaaagag aagcaggcta
    23281 cagaagcggc gatgcaactg gcgcaggatt atccggcggt atcgcctacg gagttgggca
    23341 aagagaaatt tccttttctc tcgtttcttt cccagtcgtc atcaaactat ttgacccatt
    23401 ctccctctct gctgttttcc aggaaaggga gtgaagagaa acaaaattaa tccgtcgagg
    23461 attgcgcttt tttctgcgct tcaatttcct ggcggcgcat gcgaaagaag tcactgagca
    23521 acgctgcgca ctcatccgcc agtattcctt ccgtaatttc cactcggtga ttcatacccg
    23581 gatgatgcag cacatccatt aaagatcccg cagcgccagt tttcgcgtca tgcgcaccaa
    23641 agaccacgcg accaatgcga ctgtggatca tcgctccggc acacattaca catggttcaa
    23701 gcgtgacata caacgtggcg tcgatcagac gataattttg catcaccaga ccaccctgcc
    23761 gcagggccat gatttctgca tgtgcggtgg gatcatggcg accaatcggg cggttccagc
    23821 cttcgccgat tacccgattg ttatgcacta ataccgcgcc gaccggcact tcccgctcat
    23881 cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca gtattcgtgg ctaaattcga
    23941 cttcagacaa aaagaaaact ccggttataa aagcgcggcg cattatacac ggacgctatg
    24001 ctttactcta ttccagttgc tggagttcac cgcgcggggt tactcgccaa cgatgctgac
    24061 agaaataaag cagcggattg tcctgattac tgtcgctata gccactgtac agccgcagcg
    24121 gagtgccgat tttgcgctcc agttgtgcga ccttttcatg tcccagacaa cgcatcgtca
    24181 atacccaacc accatagcca cgctgaattt ggctggcgat aagattaacc cgcggcagcc
    24241 agggcgtatc gaaataaacc gcttcaacca gcggctgcgg agagccggta atcaaccaga
    24301 tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc ggaaaggcgg
    24361 taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa cgtctgtaaa cgtgcttcgc
    24421 tgtgaccaaa agtgcacccc cacagaagca gactcatcgg ccagcgtgcc gcacgacctt
    24481 ttatcaataa cgcaatggct ataatcggta acaacggcag gacaagtaac gcattcagcg
    24541 gttggcgacg cagtaaatag cgcagaaaac tgccgaacat atcctgctga tgcaatgttc
    24601 catctaagtc aaaaaacacc acacgacgct cgtgagttgc caaaccatac tcctctggac
    24661 tgaaacatcc tgattaattt tcgctactac atagcctaac agatagatca tcacttttcc
    24721 ggcaacaatc gaccgcatca tggctaactg gaatttttaa ttcatggcaa ttagcggcaa
    24781 tggaatataa aattcactcg cgtgtgtctc atattgcctg ttgtcgccac tatttcatcg
    24841 cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg ttaccagggg cttgcccaga
    24901 gtgataaaaa actggcggat tatctgctgc tacaacctga tacggcgcgc catttaagtt
    24961 ctcagcaact ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag ttcgcgcaaa
    25021 aactcggcta taaaggtttt ccggcgctta agttggcgtt gagtgaagcg ctggcaagcc
    25081 agccggaatc gccctccgtg cccattcata accaaatccg cggtgatgat ccgttacggc
    25141 tggtcggcga aaaactgatt aaagaaaata ctgccgctat gtacgcaacg ctaaacgtta
    25201 atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg cggataattc
    25261 tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag ctgatgaaga
    25321 ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct actcgcaaca gtacaggcgt
    25381 cgtcccctga cgatctgtta ttagccattt cctacaccgg tgtacgacgc gagttaaacc
    25441 tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc ggctttactc
    25501 ccaatgccct gcaacagcgt gcttctcatt gcctgtatac cattgccgaa gaacaagcga
    25561 caaacagtgc ttcaatctct gcttgtcacg ctcagggaat gttaacggat ttgctgttca
    25621 ttgcgctgat tcagcaggat ctggaactgg caccagaacg tattcgtcag agtgaagcgc
    25681 tgatgaaaaa actggtctga gtaaagaatg cgcgtataat gcccgcccgg tttgtgttgt
    25741 tttgagagtt tccttatggc gttgttaatc actaaaaaat gcatcaattg tgatatgtgt
    25801 gagatcaaat aaaaacacat ttattttcat agcaatacag caatgtgtta ttgaatgtgt
    25861 tgttggattt atggtgcaaa aaaattttgc ataagttcag caacacaaag atcccccctt
    25921 tctataacgg ttcatttgag actccccttg actccctctt caaaattgaa gaccatcacc
    25981 tgtacataat cacagtcctg gataacttta tgcgcagaaa agagtcaaac gttagcagtt
    26041 tgccggaact caccaatttt gaggtaagct attccctagt taccaatgaa gtttacttgt
    26101 ctgcatcctt cactgataac atggcctgta taccaaactg gcccttacaa gaatttccgg
    26161 atcaactaat atgcatttct cgagcaaaag ctattgccct aatcgaagag ctccagaaga
    26221 ccatcgacta tatggacgca ggaatcgatc gacaatcagg tagcctgctc cagtagctgg
    26281 cgagttttat actcgttccc tgcaacaatg ggcttttggt aaggcactca caaataagga
    26341 attcgttaag gcttttgttc tggagactct gaaagataca aggaatcagt tgatggctgg
    26401 tttgatcata ctaattaata tattgtcaga ctcacaatat attaattagt ggtagaacat
    26461 aaaaccgagt tcttgacttt caatagctat tatatgcgat caacaacctt aaccacctcc
    26521 ttatatttta tgagatactc aaacaattta aatttatgag caaaggaaaa taataaagtg
    26581 aataaaaatc cctgggatat tccagcatgg cacgctctgg gccgagaagc atctttagtt
    26641 acacagctca ttggttctgg ggcaacagcc ataggtaaag ccaattatgc agacaaaata
    26701 ggtgaatatt acacagcatt ttttgggctt tctgtaggtt ttgagagact tgctaaacta
    26761 atacttgttg cacactatgc cattgaacac aaagggaaaa tgcctgatga gaatattgta
    26821 agaaaatttg gacataagct cattgaatta accaacgagg ttgatagtat ttctaaaaaa
    26881 atgcaactat ctctgagata ttcacgtcct acacataaaa ttacctacaa aatacttgaa
    26941 tgtttagatt cctttgctga tgcccgacgg ggacgttacg caaatttctc ctcattaagc
    27001 agccccaatt taaccaataa tgaacctatt aataaatggt ggggagaagt agccgagagt
    27061 atccttgaaa aacattatta ttatacagtt gaacaaagac gtgttgaaga aaatgctagg
    27121 tttatcaatg ccactctttc accgtataca acggtcatgt atatgaatga aagtaccaac
    27181 attatgcaag accttatatc agcctccatc cgtactggac aaaataccat tgttcaaaag
    27241 tggggaagat tctattctct tagcatcgct cgttggcttg ccacagtatt agcagaactg
    27301 tctgatatag cttctcataa atatggaata attagttttt atggccttag tgaacattgt
    27361 tgcagttata tagttgaaga tagtttttta aaaaacagaa aaatatggcc acttcgccaa
    27421 tagttaacta gaaaatctgc ataaggggga gaaatccccc acctgtaata acataactac
    27481 taaaattaac accttgttcg cacaactcct ccaccagcac gacaagatgc cgcacactgc
    27541 gccccagccg atccagcttc cagacaacca gagtgtcacc tgccgataat gtcctgagca
    27601 gttttttcag tcccggcctt tcgg
//
LOCUS       DAHBHC010000070        27171 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHBHC010000070 DAHBHC010000000
VERSION     DAHBHC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.070"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..934)
                     /gene="ycbX"
                     /locus_tag="M2A35_003532"
     CDS             complement(<1..934)
                     /gene="ycbX"
                     /locus_tag="M2A35_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCB7199778.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQ"
     gene            1178..3286
                     /gene="rlmKL"
                     /locus_tag="M2A35_003533"
     CDS             1178..3286
                     /gene="rlmKL"
                     /locus_tag="M2A35_003533"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCB7199779.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            3297..5204
                     /locus_tag="M2A35_003534"
     CDS             3297..5204
                     /locus_tag="M2A35_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7199780.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQSVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            5334..6587
                     /gene="pqiA"
                     /locus_tag="M2A35_003535"
     CDS             5334..6587
                     /gene="pqiA"
                     /locus_tag="M2A35_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCB7199781.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            6592..8232
                     /gene="pqiB"
                     /locus_tag="M2A35_003536"
     CDS             6592..8232
                     /gene="pqiB"
                     /locus_tag="M2A35_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCB7199782.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            8229..8792
                     /gene="pqiC"
                     /locus_tag="M2A35_003537"
     CDS             8229..8792
                     /gene="pqiC"
                     /locus_tag="M2A35_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCB7199783.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQITVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9048..9215
                     /gene="rmf"
                     /locus_tag="M2A35_003538"
     CDS             9048..9215
                     /gene="rmf"
                     /locus_tag="M2A35_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCB7199784.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9285..9803)
                     /gene="fabA"
                     /locus_tag="M2A35_003539"
     CDS             complement(9285..9803)
                     /gene="fabA"
                     /locus_tag="M2A35_003539"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCB7199785.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(9872..11632)
                     /locus_tag="M2A35_003540"
     CDS             complement(9872..11632)
                     /locus_tag="M2A35_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCB7199786.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            11818..12270
                     /gene="matP"
                     /locus_tag="M2A35_003541"
     CDS             11818..12270
                     /gene="matP"
                     /locus_tag="M2A35_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCB7199787.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(12346..13386)
                     /gene="ompA"
                     /locus_tag="M2A35_003542"
     CDS             complement(12346..13386)
                     /gene="ompA"
                     /locus_tag="M2A35_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCB7199788.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(13743..14252)
                     /gene="sulA"
                     /locus_tag="M2A35_003543"
     CDS             complement(13743..14252)
                     /gene="sulA"
                     /locus_tag="M2A35_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCB7199789.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            14471..15100
                     /gene="sxy"
                     /locus_tag="M2A35_003544"
     CDS             14471..15100
                     /gene="sxy"
                     /locus_tag="M2A35_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCB7199790.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15063..17225)
                     /gene="yccS"
                     /locus_tag="M2A35_003545"
     CDS             complement(15063..17225)
                     /gene="yccS"
                     /locus_tag="M2A35_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCB7199791.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17235..17681)
                     /locus_tag="M2A35_003546"
     CDS             complement(17235..17681)
                     /locus_tag="M2A35_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCB7199792.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            17804..19858
                     /gene="helD"
                     /locus_tag="M2A35_003547"
     CDS             17804..19858
                     /gene="helD"
                     /locus_tag="M2A35_003547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCB7199793.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAACHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(19890..20348)
                     /gene="mgsA"
                     /locus_tag="M2A35_003548"
     CDS             complement(19890..20348)
                     /gene="mgsA"
                     /locus_tag="M2A35_003548"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCB7199794.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(20444..21106)
                     /locus_tag="M2A35_003549"
     CDS             complement(20444..21106)
                     /locus_tag="M2A35_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCB7199795.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            21279..21692
                     /locus_tag="M2A35_003550"
     CDS             21279..21692
                     /locus_tag="M2A35_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCB7199796.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSSKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(21737..22054)
                     /gene="hspQ"
                     /locus_tag="M2A35_003551"
     CDS             complement(21737..22054)
                     /gene="hspQ"
                     /locus_tag="M2A35_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCB7199797.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22112..23302)
                     /gene="rlmI"
                     /locus_tag="M2A35_003552"
     CDS             complement(22112..23302)
                     /gene="rlmI"
                     /locus_tag="M2A35_003552"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCB7199798.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEDGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVSATYPEGLYLKGFA
                     CRVM"
     gene            23397..23675
                     /gene="yccX"
                     /locus_tag="M2A35_003553"
     CDS             23397..23675
                     /gene="yccX"
                     /locus_tag="M2A35_003553"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCB7199799.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLVQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(23672..24001)
                     /gene="tusE"
                     /locus_tag="M2A35_003554"
     CDS             complement(23672..24001)
                     /gene="tusE"
                     /locus_tag="M2A35_003554"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCB7199800.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAKNEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24092..24751)
                     /gene="yccA"
                     /locus_tag="M2A35_003555"
     CDS             complement(24092..24751)
                     /gene="yccA"
                     /locus_tag="M2A35_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCB7199801.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25159..26178)
                     /locus_tag="M2A35_003556"
     CDS             complement(25159..26178)
                     /locus_tag="M2A35_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7199802.1"
                     /translation="MGRPRKDKKDNVLPPRVRSNGYSYVWKPKGSTRTIKLGRVRETS
                     VAKVWQNYELEKAKLHDIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     MLADNVKIEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKAGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26147..26398)
                     /locus_tag="M2A35_003557"
     CDS             complement(26147..26398)
                     /locus_tag="M2A35_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCB7199803.1"
                     /translation="MYELTLSPAEIQEITKYKRYTRQQHQLRLHGIPFLIGPKNEPIV
                     LRCDIPRGLTAMSKVSELVSADPDFEALNNGKTKKRQKR"
     gene            complement(26465..27016)
                     /locus_tag="M2A35_003558"
     CDS             complement(26465..27016)
                     /locus_tag="M2A35_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCB7199804.1"
                     /translation="MIDLETMGTNTNAPIVVIGAVFFDPQTGEIGPVFYIVISLTDAM
                     NTGAVPDGGTIEWWLKQSSEARAAILTDQVKLKDALSRFREFINEYSDEKFVQVWGNG
                     ATFDNAILRTSYERLDIPCPWRYHNDRDVRTIVELGKTIDFDARTFIPFEGVRHNALD
                     DARHQAKYVTATIQKMIPSQADF"
BASE COUNT         6880 a         6608 c         7087 g         6596 t
ORIGIN      
        1 attgctccag caacacctgt tggttatttc cacgaaatgc ctgtccctgc cagtcaatat
       61 ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt
      121 aaatttttgc cggagccgtt gccagaattt ccacctcatc gccaacgcga atcacgccgc
      181 tattacgggc aattaaattc tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga
      241 aagattgcaa tgtttttaat ggttcgcctg ccggatgttt ttgccctttt tctgggctga
      301 cggtggtgaa aatacagcgg ctacaaggtt taaccacatc aaacaccaca tcaccaatgc
      361 gaatcacttt ccagctatct tcttcccagg ctgacgcgcc ggaaaccacc agattggggc
      421 ggaattgctc catttttaca ctggccggac aacgttgttg gagatcacgt aacgaggctt
      481 cgttagcaag aaggtaagga tagccatcag caaatgacag gggtacagtg ttgtggcgtt
      541 tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc
      601 cacttagcca tttgttgatc gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa
      661 cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg ctactgccat
      721 ctggtgcggt gagatgcaag ccatcatgca caggtgaagg agtaaaccgt accatctggg
      781 gaaactggcg agcggtaata aacgtaccgt caggttccgt gatcataaag atgcgatcga
      841 aggccagacc actgacatca gccagagcat gtgtaagacc aatgccgcgc atcgatttaa
      901 caggatgaat aaaaagccgg gttaatgtca ccacggtgcg gtcctcaaat gaaaataagc
      961 cctcaacttt atgacatgac gcgcttatta gctataatgc gcaacaattt tcttagcgcc
     1021 tgtcccatta ggctatttta tttgtcagtt tggccctggg ttgtgctcga aatcctcacg
     1081 tacttgtgta cgctccggtt tctccgcgct gtccatgtcc agactgactg caacaattac
     1141 gcctactgcg gtaggctctt agagtaaaag tgacgatatg aattctctgt ttgccagtac
     1201 ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa aacctggggg ccgttgaatg
     1261 ccaggtggtt cagggtgggg tccatttcaa gggcgacaca cggcttgttt accagagcct
     1321 gatgtggagc cgcctggcct cgcgtattat gttgccgctg ggcgagtgta aggtttacag
     1381 cgatttagac ctctatctcg gtgttcaggc gatcaactgg acagagatgt ttaatcctgg
     1441 cgcgaccttc gctgtccact tcagtggttt gaatgacacc atccgcaaca gtcagtacgg
     1501 cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc
     1561 aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc tggctgcata aagaaaccgc
     1621 cagtatcgct cttgatctga gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc
     1681 tggtattgcg ccgatcaaag aaaccctggc agccgctatt gtgatgcgat ccggctggca
     1741 gccaggaaca ccgctgctcg atccgatgtg tggttccggt acgttgctga ttgaagcggc
     1801 gatgctagcg accgatcgcg caccaggctt gcaccgtggg cgttggggct ttagcggctg
     1861 ggtgcagcat gatgaagata tctggcagga agtgaaagcg gaagcgcaaa ctcgtgcccg
     1921 taaaggcctg gctgagtata gctctcattt ttacggttcg gacagcgacg cacgggtgat
     1981 tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt
     2041 gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg ccgtacggca cagtgttgag
     2101 caacccgcca tacggggaac gtctggacag cgaaccggcg ctgattgcgc tgcatagcct
     2161 gctgggtcgg atcatgaaaa accagtttgg tggctggaat ctctctttgt ttagtgcctc
     2221 gccggatctg ctaagctgct tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg
     2281 cccgctggac tgcgtacaga aaaattacca cgttgccgaa agcactccag acagcaaacc
     2341 ggcgatggta gcggaagact acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa
     2401 gtgggctcgc caggaaggaa ttgaatgtta ccgcctgtat gacgccgatc taccggaata
     2461 taacgttgcc gttgaccgtt atgccgactg ggtggtggtg caggagtatg cgccgccaaa
     2521 aactattgat gctcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc
     2581 ggtactgggg attgcgccaa acaaactggt gctgaaaacc cgtgaacgcc agaagggcaa
     2641 aaatcagtac cagaaactgg gcgagaaggg cgagtttctt gaagttaccg aatataacgc
     2701 tcacttgttg gtgaacctga cggattacct cgataccggt ctgtttctcg atcaccgtat
     2761 cgcccgtcgt atgctcggtc agatgagcaa aggcaaagat ttcctcaacc tgttctctta
     2821 taccggcagc gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga
     2881 tatgtcgcgt acttatctgg agtgggcaga acgcaacctg cgtttgaatg gcctgaccgg
     2941 gcgtgcgcat cgcctgattc aggccgattg cttggcgtgg ctgcgtgagg caaatgaaca
     3001 gttcgatctg atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc
     3061 gtttgatgtt cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc
     3121 aggtgggacg attatgttct cgaacaataa acgtggcttc cgtatggatc tcgacggcct
     3181 ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc
     3241 ccgtaaccgc cagatccaca actgctggct gattaccgca gcctaaagga atagtaatgt
     3301 cattaatcag tatgcatggc gcatggctgt cgttcagcga cgcgccgctt ctcgataacg
     3361 cagaactgca tatcgaagat aacgaacgtg tttgtctggt gggccgcaac ggcgcgggca
     3421 aatcgacgtt aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt
     3481 acgagcaaga tttgattgta gctcgtctgc aacaggatcc gccgcgtaac gttgagggta
     3541 gcgtttatga tttcgttgcc gaaggcattg aagaacaagc ggaatatctg aaacgctatc
     3601 acgatatttc gcgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga
     3661 aggttcagga acagctggat caccacaacc tgtggcagct ggaaaaccgc atcaacgaag
     3721 tgctggcgca actggggtta gatcctaacg ttgcgctgtc gtcgctttcc ggcggctggt
     3781 tgcgtaaagc ggcattagga cgcgcgctgg tgagtaatcc gcgcgtgctg ttgcttgatg
     3841 aaccgacaaa ccacctggat attgaaacca tcgactggct ggaagggttt ttgaaaactt
     3901 tcaacgggac gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc
     3961 gcattgttga tctcgatcgc ggcaagctgg tgacctatcc agggaattac gaccagtacc
     4021 tgctggaaaa agaagaagcc ctgcgcgtgg aagaattaca aaatgccgag ttcgatcgca
     4081 aactggcgca ggaagaggtg tggatccgcc aggggatcaa agcacgccgt acccgtaatg
     4141 aaggccgcgt acgcgccctg aaagcgatgc gtcgcgagcg tggtgaacgt cgcgaagtga
     4201 tgggtaccgc aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa
     4261 tggaagacgt ttgctaccag gttgacggta agcaactggt gaaagatttt tcggcccagg
     4321 ttctacgtgg cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa accacgctgc
     4381 tgaaactgat gctcggtcag cttcaagcgg acagcgggcg tattcacgtt ggcaccaaac
     4441 tggaagtggc ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg
     4501 ataaccttgc cgaaggtaag caagaggtga tggttaacgg caagccacgc cacgtattgg
     4561 gctatttgca ggactttctg ttccatccga aacgggcgat gacgccggta cgtgcgcttt
     4621 ctggcggtga gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat
     4681 tgattcttga cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac
     4741 tgatcgacag ctatcagggc acggtattgc tggttagcca cgatcgtcag tttgtcgata
     4801 acaccgttac agaatgttgg atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg
     4861 gttatcatga tgcccgtggt cagcaagagc agtctgtggc gctcaaacag cctgcggtga
     4921 aaaaaaccga agaagccgcc gcggcaaaag cagaaactgt aaaacgcagc agtagcaaac
     4981 taagctataa attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg
     5041 cgaagctgga agccctacag acgcaggtgg cggatgcttc cttcttcagt cagccacatg
     5101 agcagacaca aaaagtgctc gccgatatgg ctgctgcaga gcaggagctg gagcaagcct
     5161 ttgaacgctg ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat
     5221 ttaaacagag acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga
     5281 aaaccaacac tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg
     5341 aacatcatca tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac
     5401 cgcgccttga gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg
     5461 cgtgggatgc ccctcggcag cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt
     5521 tgctgtccaa cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta
     5581 cattactgga aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt
     5641 tcctgttgtt tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga
     5701 atcgcgcgga attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca
     5761 aaacctgggg aatggcggag attttcctcg ctggtgtgct ggtcagtttc gttaaactga
     5821 tggcttacgg cagcattggg gtaggcagca gctttctccc ctggtgttta ttttgtgtcc
     5881 tgcaactgcg cgcttttcag tgcgttgatc gtcgctggtt atgggacgac atcgccccga
     5941 tgccagaact gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt
     6001 cgtgctcctg ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta
     6061 ccaaagggta cgttcggcgt agaaacagcc tgcagtggac actcgcgctg cttgtaacgt
     6121 ccatcatgct gtatcttccg gctaatattt tgcccatcat ggtgacggat ttattaggct
     6181 cgaagatgcc gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa ggctcttatc
     6241 ccgtcgctgc ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca
     6301 tcgcgtggct gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt
     6361 tgatttatga agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg
     6421 cggtgctctc ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg
     6481 cattaatgtt tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc
     6541 gtttgtcgtg ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct
     6601 aataatgggg aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct
     6661 atcgtcacgg cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg
     6721 gaagtgaccc tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa
     6781 agccgtagcg ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacgcac
     6841 gttgaaatca aagcgcggct gaattccggt atggaaaaat tgctgcataa agacaccgtc
     6901 ttttgggtgg tgaaaccgca gattggtcgc gaagggatta gcggcctggg aacgctgctg
     6961 tctggagttt atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat
     7021 ttgctggact cgccaccgtt ggccccgcct gatgcgaaag gtatccgtgt gattctcgat
     7081 agcaaaaaag ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta
     7141 ggttcggttg aaaccagcac cttcgataca caaaaacgca atatcagcta tcaactgttc
     7201 atcaatgcac cttatgaccg actggtgacc aacaatgttc gcttctggaa agatagtggc
     7261 attgcggttg atctgacgtc agcagggatg cgtgtggaga tgggctcatt gacaacgctg
     7321 ctgagtggcg gtgtcagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca
     7381 ccgaaaacag ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc
     7441 gatcacattg attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct
     7501 ccggtagagt tccggggtat tcgcctgggt accgtaagca aagtgccatt ctttgcgccg
     7561 aatatgcgtc agacatttaa cgatgattac cgtattccgg tactgattcg tatcgagcca
     7621 gagcggctga aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg
     7681 ttgaaacgtg gtttacgcgg atcgctgaaa accggaaacc tggtcactgg tgcactgtat
     7741 gttgatctcg atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt
     7801 tatcagatta tcccgaccgt tagcggcggc ctggcgcaaa tccagcaacg actgatggaa
     7861 gcgttggata agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg
     7921 ctttctgaaa gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag catgaacaag
     7981 atcctcgcta gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa
     8041 ttgaatcgca gcatgcaggg cttccagcct ggctccgcag cctacaacaa gatggtggcg
     8101 gatatgcagc gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat
     8161 gagaagagta acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg
     8221 gcgaaacaat gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct
     8281 ccggcgaaat taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta
     8341 ccgccagcca gggcaatcgt ctgttatggg tagagcagat cactgttcct gactatctgg
     8401 cggggaatgg tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aacaacaact
     8461 tgtgggccag cccgttggat caacagttgc gcaacaccct ggttgccaac ctgagtacgc
     8521 aactgcccgg ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg
     8581 ttaccgtaac ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc
     8641 tgttgaacca ccagggacaa ctgatcaaac gtccgttccg tctggaagga gtgcaaactc
     8701 aggatggtta cgatgagatg gttaaagtgc tggccggtgt ctggagtcag gaagccgctt
     8761 ctattgcaca agagataaag cgtctacctt aattataaag atttgtaaat ataaccgtct
     8821 ccggtatgtt gcctgaggcg gtttttttgt ctctaacgtg cggaaaaatt tgttcctctt
     8881 cacatttttt gtacaaccga catgcccgtg tagctcacaa atatgacagt ggcgtgaatt
     8941 ttgcgcattg acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta
     9001 atcactgttt tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa
     9061 aacgagatcg cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct
     9121 caaaagaaat gtgtccctat cagacgctga atcaaaggtc acaatggctg ggaggctggc
     9181 gagaagccat ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc
     9241 cgcattgcgg aggtttcgcc ttttgatact ctgtctgatt ataatcagaa ggcagacgta
     9301 tcctggaaca gaccgacttt caggtcgctg gcggtataga tcagacgacc atcaaccagc
     9361 acttcgccat ccgccaggcc cataatcaga cgacggttaa caatgcgttt aaagtgaata
     9421 cggtaggtca cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg
     9481 cccaacgcgc ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc
     9541 cacattgcgt ccaggcccag gcatcccggc ataaccggat cgccaataaa gtggcatccg
     9601 aagaaccaca gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta
     9661 ccacccgttt cggtcatttt gaccacacgg tccatcatca gcatgttcgg tgctggcaat
     9721 tgcgggcctt tagcgccaaa cagttcaccg cgaccagagg caagaaggtc ttcttttgta
     9781 taggattcgc gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga
     9841 tagctaacac gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac
     9901 gtaatggcca tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct
     9961 ggatggtttg catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg
    10021 gcagtgcgtc agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt
    10081 tcaccagttc actgtgaaga cttaaatggc gaacgttagc tgtggggata atgacacctt
    10141 gtttcccggt taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta
    10201 aaccaccgac cggctgggcg cgaccgaact gatcgactga acctgtgata gcgatactct
    10261 gattcaccgg cacatcggcg agggcgctta tcagggcgca gagttcagcc atcgaggcac
    10321 tatctccatc aacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct
    10381 gttgctcaag ctgtagttcc gacatcagga acgcttgcat gatcatcatc cctttcgcat
    10441 ggatattgcc gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat
    10501 gcacaacgca gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa
    10561 tgaccgaaag ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct
    10621 cctgaaggat ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct
    10681 gcagcattaa gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct
    10741 ctttacactg gcggaggatc cactgcgggc taagcggaag tgtttcttgt tcaccggtgt
    10801 agcgtgctgc ttcgcggata agtaccggcc aggcatccgc tcccggtgca ggtaagtgat
    10861 tatgtctggc ggtaaatgtc acccagcgac accactgggt tactgactcc gcatcgacaa
    10921 tctgcagagt atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct
    10981 cctggaaatc agccaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca
    11041 tcgaaggcac agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct
    11101 cgcggttaac gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca
    11161 aagagatgat gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt
    11221 cgccattaaa ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga
    11281 caacttgcgt taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg
    11341 agtggccgga aacctcatag tgaccgccca ccagttggcc tgcatcgctt tgtagcgtac
    11401 gcgcggcatt ggcaataaga ttcagatact cagactcttc cggggccttc gccagcataa
    11461 aagaggagga tgctcgcgta tgcagcaact gctccagtgc gaattgcagt cgcggttgag
    11521 tatcactgaa taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat
    11581 agctatcggt atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg
    11641 ttttttagtc tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga
    11701 aagcaccgac aatactcctg gcatgggcgt taaagctcac aggatggaga ttcttttctt
    11761 cactggccta aaaagctgat attctgtaaa gagttacacg gtaacattga gatcgctatg
    11821 aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag
    11881 catcgcgaag gggagttaat cacccgttac atagaagcca gtgccgccca ggaagccgtc
    11941 gatgtgttgc tctcgctcga aaatgaaccc gtgctggtaa atggctggat tgacaagcat
    12001 atgaatccgg aactggtcaa tcgcatgaag caaaccattc gggcaagacg aaagcgccat
    12061 tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg atctggaatt tattgtctgg
    12121 caacgtctgg ctggtcttgc gcagcgtcgc ggtaaaacgc tttctgaaac gattgttcag
    12181 ctgattgaag atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc tctgaagcag
    12241 gatctgcagg cattgctggg taaggaataa ctgacgaaag tcagttcaat ttactaaagg
    12301 caaaaaaaac cccgcagcag cggggttttt ctaccagacg agaacttaag cttgcggctg
    12361 agttacaacg tctttgatac ctttaacttc gatctctacg cgacgatccg gagccaggca
    12421 gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga
    12481 ttcgcccata ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc
    12541 aacaacagac tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg
    12601 gtcggtgtaa cccagaacaa ctacggaacc gtctttcgga tccaggttgc tcagctggct
    12661 gtacagctga tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa
    12721 cagaacgtca gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc
    12781 cggagcaact actggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag
    12841 catgccgttg tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt tggtccactg
    12901 gtattccaga cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc cagcgaagac
    12961 cggagaaacg ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt ctgcacgcca
    13021 taccatacca cccagacgag tgtagatgtc caggtcgtca gtgattgggt aacccagttt
    13081 agcggtcagt tgaacgccct gagctttgta tgcaccgttt tcaacgctgc ctttgtacgg
    13141 catacgacct aaccagtcgt aacccatttc aaagccaaca tacgggttaa cctggtaacc
    13201 accaaaagca ccagcgccca gttggttttc atgggtcggg ccattgttgt tgatgaaacc
    13261 agtgtcatgg tactgggacc agcccagttt agcaccagtg taccaggtgt tatctttcgg
    13321 agcggcctgc gctacggtag cgaaaccagc cagtgccact gcaatcgcga tagctgtctt
    13381 tttcattttt tgcgcctcgt tatcatccaa aatacgccat gaatatctcc aacgagataa
    13441 cacggttaaa tccttcaccg ggggatctgc tcaatattaa ctctaccgat atcttcggct
    13501 tatgccgagc acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga
    13561 actccgtcag gcatatgaaa aaaaagtctt gtataaggta tgtttaatct tttttgtcag
    13621 cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt
    13681 agtcacgact gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt
    13741 acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt ggcgtgagag
    13801 gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca
    13861 agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa
    13921 ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaagggga gagctggcta
    13981 atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt
    14041 tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc
    14101 aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc
    14161 ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac
    14221 gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt
    14281 atggatgtac agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg
    14341 caaattttgt cgttggtgac gggaaaacat aaattaatct tgccccttaa gaataagttg
    14401 cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa
    14461 aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc
    14521 aacgttgggc acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgacga
    14581 cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc
    14641 acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt
    14701 taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg
    14761 tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga
    14821 aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg
    14881 gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg
    14941 gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg
    15001 cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc
    15061 tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca
    15121 atctcaggca gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg
    15181 tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg
    15241 cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca
    15301 agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata
    15361 tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg
    15421 gtaacgttcg gttcgctgga catatttgat accaccgacg ccagttcagc atcacggttg
    15481 tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc
    15541 tccagtatgg catcgagata ccggcagttg gcctctgtgg cgcgttcgag cattcgcggc
    15601 agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa
    15661 ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagcaagtta
    15721 aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gggcatattg cacgttacgg
    15781 aaggcaaaaa agagcacgcc ggtaataacc agtagcacca gctgcccttc cagtgacggt
    15841 acaaaccaca gcacaggaat gccaatggcg ataccgacca gcgtaccaat aatccttaac
    15901 ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag
    15961 atccaatacc cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa gcacaacacc
    16021 agcgacattc ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga
    16081 cgcagccaga tatcactcaa tccgtgcggg ctatcatcag cgagctcatt ttcgtcatta
    16141 ttatggggca gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg
    16201 cgtaaattgt tcagcaaaaa tcccagtgtt ttaagtaaat cggcaggcgc gccgttatcg
    16261 cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt
    16321 tgataaggct gacgcagcaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc
    16381 gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt
    16441 tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa
    16501 tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag
    16561 agtttcgtct gattcaatgt cgccatcagc tgaccgttgg cgagagccaa atcgtacagc
    16621 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
    16681 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
    16741 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc
    16801 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
    16861 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
    16921 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
    16981 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
    17041 cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt
    17101 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
    17161 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
    17221 gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgcc
    17281 gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag
    17341 ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg
    17401 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
    17461 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
    17521 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag
    17581 gtaaaaatca gcataatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
    17641 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
    17701 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
    17761 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
    17821 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
    17881 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
    17941 tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt
    18001 ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
    18061 tcactggcag cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca
    18121 actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg agcaaacctc
    18181 tggcgttcag caacaaatcc gccaggctat gtcggcgttg ccgttgccgg ttaaccgact
    18241 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat
    18301 tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc
    18361 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt
    18421 taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct
    18481 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
    18541 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg aacggctaca
    18601 taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg
    18661 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgcct gtcatgaact
    18721 ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
    18781 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga
    18841 aaaattacag cgtcgtcttg catcacgcct cgatcgttgg gtaagtctga tgcggatgca
    18901 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
    18961 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
    19021 aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggtcg
    19081 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
    19141 ggcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg
    19201 tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt
    19261 ccatgaaaac tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag
    19321 tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagt tgaaaaagcc
    19381 gctaaacagc ttaaccaatg gagacgaaaa agccgtcacg ttattggatg agagtcaact
    19441 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttcttatcct
    19501 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
    19561 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat
    19621 catcgttggc ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat
    19681 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
    19741 gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc
    19801 ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtagcgagaa aaccgtaaga
    19861 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
    19921 taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat tatgaagtct
    19981 gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg caacaaggct
    20041 ttcacgtcag gatcgtgcgg cacggcattt agtgggtccc agaagaaaat caatacatca
    20101 attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac
    20161 atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt gcctgttgca
    20221 tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt
    20281 ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc
    20341 agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct
    20401 gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt
    20461 tttccgccca ttgtaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact
    20521 tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac
    20581 ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt
    20641 tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg
    20701 taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa
    20761 ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa
    20821 aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca
    20881 gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg
    20941 gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc
    21001 cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata
    21061 ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc
    21121 aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat
    21181 agacatattt cccgcttttg ctctcaatca ttcgtattag ctgcatggtt ggcatgtcgg
    21241 ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag
    21301 gtattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc
    21361 ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgt
    21421 caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg gcagacgttc
    21481 cggaaaaagt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcgc
    21541 aggaagctat cgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc
    21601 aagcggcagt actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta
    21661 ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg
    21721 gggatttgtt cagggattag ttacgcagac gcggggcctg gagttgtttg cggatggtct
    21781 gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct
    21841 gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc accacgtgat
    21901 accacggagc ggcgcgaagc tcgtcattca ccgccagttc atcaggcgat ggttcagaga
    21961 gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga
    22021 cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct
    22081 ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca
    22141 gatatagccc ttccgggtag gtagcgctca ccggatgatc ggctgcctga cggaactgct
    22201 ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata
    22261 aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgtct tcattcagca
    22321 actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc aactggcttt
    22381 tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc
    22441 gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca
    22501 gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc
    22561 tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga
    22621 aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct
    22681 ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt
    22741 gttcttcaat cggcagcagg gcaggcggca actcgccggt gacggggcct tgggtcagct
    22801 ccatcccttc ttttttacgt accgcaacat cgctgcgatc gtaaatcgca cattccgggt
    22861 acagcgtttg cagggcacta attaatgccg cgcgctgata ctctgcccca gcactcagca
    22921 gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt
    22981 ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt
    23041 tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa
    23101 acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt
    23161 ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg
    23221 caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg
    23281 ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat
    23341 tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt
    23401 cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca
    23461 ccacacagta cgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg
    23521 gcagcgtgga agtggttgct tgcggtgaag aagggcaggt agaaaaatta gtgcagtggt
    23581 taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc
    23641 cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc
    23701 cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat aaatagcggc
    23761 tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg
    23821 gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttctg
    23881 gcgacagcga aatcccttcg ttctttgcaa tcaccactgc cagcggttca ctccactggc
    23941 tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca
    24001 taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa
    24061 agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag cccagaatgc
    24121 tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat
    24181 agttcgtttc accgccatga atgatgttgc tggtttcaaa caaaatagcg ccagaggaga
    24241 tcagaatgaa gaccgcgctg atcgccagat gcagagcagg cagttgcagg aagatattag
    24301 caaccatacc aatcagtacc accacaatac ccgccatcag cataccgccg aggaacgaca
    24361 tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc
    24421 ccagtgccat agcgattacg tcacccattc cggcagacag ataggtgttc agaattggtc
    24481 cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat
    24541 tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac
    24601 ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa aaggccagcg
    24661 tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac
    24721 ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta
    24781 attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat
    24841 ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga
    24901 gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccctta tcttatctgg
    24961 cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccgaag
    25021 aaaaataaag aaaaatcagt ataatacgca aaatagctga aaatttaatc agacttaaca
    25081 ccaagaaaaa tcaagatgtt agggagttat tcagagttaa tataccaggt ctttttgcca
    25141 gaaaattttc tgtattgcct acttactgac cacaagcggc aaatccagtg ttggcgtgat
    25201 tttggtttta cgatcgtaaa tcaacacctg attttctgtt ttgtgtccac tgaaaatttg
    25261 tttgtcgcga ctgctgcctt cgtaatctga aatccctttg gcttttatgt catggaagtt
    25321 gcacccaaac ggaacgccgg ctttttgctc ggctgcacgt ttagcctgat tccaccagtt
    25381 gttcagcgtc ttggctatta ccttcccgcc tttggttgtg ttgatcacat attcgcatgt
    25441 gccggaagat acatttcggg ctaactggat cgccgtacgt aatcgtggag accattcctt
    25501 gatttgtttg gtgccggtct tgttttgctc aatgtaaatc cctttgtcca taatatcctg
    25561 ccatttcagc tcgagcacat caccgagcct tgccgcacag agataggata tctccattgc
    25621 aatacgtaac tgtggaattg cttccgcata tatcgcagca tactgttcat cggtgatgta
    25681 aacagtacgg gctttaagcg tgaattttct gactcctttg catggattat ttttcacata
    25741 cccacgctca tatccccatc cgtatacgcg actcagactt gccagttcat ggtttgcctg
    25801 ggtcttgctc tcaagacccc gcttatccat gaaaattctt acctgctcaa tttttacatt
    25861 atcagcaagc attttcccga ataccgccag taatgccctt tgatgttgcc gataatcttt
    25921 ttgggttcgg ggggccagtt ctgtaaatgc aggggagtcc ataaacatgt gccataattt
    25981 agctacggtc attatgtcgt ggagttttgc cttttccagt tcataatttt gccagacttt
    26041 agccacgctg gtttcccgca ctcttccgag ctttatcgtt cttgtgcttc ctttgggctt
    26101 ccatacgtaa ctgtaaccat tcgatctaac gcgcggtggc agtacattat cttttttgtc
    26161 ttttcttggt cttcccatta ttcagtgcct caaaatcggg gtcagcagaa accagttcag
    26221 atacttttga cattgctgtt agtccacgtg gaatatcaca acgtagaact atcggctcgt
    26281 ttttaggccc gattaaaaat gggatgccgt gcagtcttaa ctggtgttgc tgtcttgtgt
    26341 atcgcttgta tttcgtgatc tcttgaatct ctgctggcga tagagttaat tcatacatgt
    26401 ggtcacgtgc cttacagcat gaccgccgcc aatataattc ggaggcggcg atcagggttg
    26461 aacattaaaa atcagcctga ctcgggatca ttttttgtat cgtggctgta acgtattttg
    26521 cctggtgacg ggcgtcatcc agtgcattgt ggcgcacgcc ttcgaatgga ataaaggttc
    26581 tggcatcaaa gtcgattgtt tttcccagct caacgattgt gcgtacatcg cgatcgttgt
    26641 ggtagcgcca cgggcagggg atgtccagac gttcgtatga ggtgcgcaaa attgcgttat
    26701 cgaaagttgc accattaccc catacctgaa cgaatttttc atctgagtat tcgttgatga
    26761 actcccgaaa ccgcgaaagg gcatccttca gttttacctg gtctgttaaa atggcagctc
    26821 tggcttcact ggactgcttc agccaccatt cgatggttcc accgtcagga acagcccctg
    26881 tattcattgc gtcagtcaga ctgataacga tataaaatac tggcccgatt tcccctgttt
    26941 gtgggtcgaa gaaaaccgca ccaataacca cgatgggcgc attggtgttg gttcccattg
    27001 tttcaaggtc gatcatcagg tggtaccaca ctctgctggt ggatgtgata atgtgatgac
    27061 cgttcaccgc aattaaggga tctgccgtct cgccagtttc attatcgctg gcgtggtcct
    27121 gagcgctgcc agcattctcc ttgtgtggat gttcagcgcc ttccatttcc t
//
LOCUS       DAHBHC010000071        26482 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHBHC010000071 DAHBHC010000000
VERSION     DAHBHC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.071"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            212..1255
                     /gene="nadA"
                     /locus_tag="M2A35_003559"
     CDS             212..1255
                     /gene="nadA"
                     /locus_tag="M2A35_003559"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCB7199805.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNEEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1293..2012
                     /gene="pnuC"
                     /locus_tag="M2A35_003560"
     CDS             1293..2012
                     /gene="pnuC"
                     /locus_tag="M2A35_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCB7199806.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="M2A35_003561"
     CDS             complement(2009..2950)
                     /gene="zitB"
                     /locus_tag="M2A35_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCB7199807.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3064..3444)
                     /locus_tag="M2A35_003562"
     CDS             complement(3064..3444)
                     /locus_tag="M2A35_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCB7199808.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3760..4812
                     /gene="aroG"
                     /locus_tag="M2A35_003563"
     CDS             3760..4812
                     /gene="aroG"
                     /locus_tag="M2A35_003563"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCB7199809.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="M2A35_003564"
     CDS             complement(4970..5722)
                     /gene="gpmA"
                     /locus_tag="M2A35_003564"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCB7199810.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5927..6967)
                     /gene="galM"
                     /locus_tag="M2A35_003565"
     CDS             complement(5927..6967)
                     /gene="galM"
                     /locus_tag="M2A35_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCB7199811.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6961..8109)
                     /gene="galK"
                     /locus_tag="M2A35_003566"
     CDS             complement(6961..8109)
                     /gene="galK"
                     /locus_tag="M2A35_003566"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCB7199812.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8113..9159)
                     /gene="galT"
                     /locus_tag="M2A35_003567"
     CDS             complement(8113..9159)
                     /gene="galT"
                     /locus_tag="M2A35_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCB7199813.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9169..10185)
                     /gene="galE"
                     /locus_tag="M2A35_003568"
     CDS             complement(9169..10185)
                     /gene="galE"
                     /locus_tag="M2A35_003568"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCB7199814.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10446..11918)
                     /gene="modF"
                     /locus_tag="M2A35_003569"
     CDS             complement(10446..11918)
                     /gene="modF"
                     /locus_tag="M2A35_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCB7199815.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            complement(11986..12774)
                     /gene="modE"
                     /locus_tag="M2A35_003570"
     CDS             complement(11986..12774)
                     /gene="modE"
                     /locus_tag="M2A35_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCB7199816.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12903..13052
                     /gene="acrZ"
                     /locus_tag="M2A35_003571"
     CDS             12903..13052
                     /gene="acrZ"
                     /locus_tag="M2A35_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCB7199817.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13219..13992
                     /gene="modA"
                     /locus_tag="M2A35_003572"
     CDS             13219..13992
                     /gene="modA"
                     /locus_tag="M2A35_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7199818.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13992..14681
                     /gene="modB"
                     /locus_tag="M2A35_003573"
     CDS             13992..14681
                     /gene="modB"
                     /locus_tag="M2A35_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCB7199819.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSILHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14684..15742
                     /gene="modC"
                     /locus_tag="M2A35_003574"
     CDS             14684..15742
                     /gene="modC"
                     /locus_tag="M2A35_003574"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCB7199820.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15743..16561)
                     /gene="ybhA"
                     /locus_tag="M2A35_003575"
     CDS             complement(15743..16561)
                     /gene="ybhA"
                     /locus_tag="M2A35_003575"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCB7199821.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16716..17711
                     /gene="pgl"
                     /locus_tag="M2A35_003576"
     CDS             16716..17711
                     /gene="pgl"
                     /locus_tag="M2A35_003576"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCB7199822.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCINELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17752..18705)
                     /locus_tag="M2A35_003577"
     CDS             complement(17752..18705)
                     /locus_tag="M2A35_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199823.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKSLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            18889..19941
                     /locus_tag="M2A35_003578"
     CDS             18889..19941
                     /locus_tag="M2A35_003578"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCB7199824.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20017..21450
                     /locus_tag="M2A35_003579"
     CDS             20017..21450
                     /locus_tag="M2A35_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCB7199825.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21633..23894
                     /locus_tag="M2A35_003580"
     CDS             21633..23894
                     /locus_tag="M2A35_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCB7199826.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24036..25319)
                     /locus_tag="M2A35_003581"
     CDS             complement(24036..25319)
                     /locus_tag="M2A35_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCB7199827.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRAFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25454..26473)
                     /locus_tag="M2A35_003582"
     CDS             complement(25454..26473)
                     /locus_tag="M2A35_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7199828.1"
                     /translation="MRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQANIELFSGHKH
                     KPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAIRRGLPDAPLEDI
                     TTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRAAKSEVRR
                     SRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGNLCEMKWSDIVDGYLYVEQSK
                     TGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRAR
                     KASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWD
                     KIEIK"
BASE COUNT         6321 a         6515 c         6845 g         6801 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatcttg atagtttggg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggca gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagt ccatcgcaga
     1141 ggcattagaa caggaaggaa gcaatgaaga ggttcatgtt gatgaaaggc tgcgagagag
     1201 ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataacgaat
     1261 aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg cagaatatcc
     1321 tggtacatat accaataggg gcaggcggtt atgatctctc atggatcgaa gcggtaggca
     1381 cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc aactacttct
     1441 ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca
     1501 gcctgctatt acaggtgttt ttctttgccg cgaatattta cggttggtat gcgtggtcgc
     1561 gacaaaccag tcagaacgag gcggagttga aaattcgctg gttgccattg ccgaaggcac
     1621 tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg
     1681 tgtttgcatt tttgacccgc gtggcagtca tgatcatgca agcattagga ttacaggttg
     1741 tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg atggtgttat
     1801 ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg ttgtgggtga
     1861 ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg
     1921 agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc aacagcgcac
     1981 gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc
     2041 cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctga atggtggcgt
     2101 gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag gcatcgtgat
     2161 cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct accatccata
     2221 catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga cgcttcagtt
     2281 cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta tctttcaaca
     2341 gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg gggtcagcag
     2401 gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc gaacccagca
     2461 ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct tcgctgccgt
     2521 gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca attgccatca
     2581 tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc cagacaatca
     2641 aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg gtcagtctta
     2701 gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg gcgagtaggg
     2761 cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc aatgccagag
     2821 aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc acgccgaaag
     2881 catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg tgtgagtgtg
     2941 agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc
     3001 ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca
     3061 gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccggt
     3121 ttgtaccttt ttattgatat ccggacagcg accatctttg cacatggtgt tcttgtgctc
     3181 ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca gcattgtgcc
     3241 accagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc
     3301 gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat tagtttgcgc
     3361 tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcagaaataa
     3421 tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc
     3481 tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt cagccaggtt
     3541 atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt
     3601 gtaggagaga tctcgttttt ctcgacaatc tggcgttttt cttgctaatt ccaggattaa
     3661 tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat
     3721 tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat
     3781 ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc
     3841 gctactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca taagatcctg
     3901 aaaggtaatg atgatcgcct gttggttgtg attggcccat gctcaattca tgatcctgtc
     3961 gcggcaaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg
     4021 gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggctg
     4081 attaacgatc cgcatatgga taatagcttc cagatcaacg acggtctgcg tatagcccgt
     4141 aaattgctgc ttgatattaa cgacagcggt ctgccagcgg caggtgagtt tctcgatatg
     4201 atcaccccac aatatctcgc tgacctgatg agctggggcg caattggcgc acgtaccacc
     4261 gaatcgcagg tgcaccgcga actggcatca gggctttctt gtccggtcgg cttcaaaaat
     4321 ggcaccgacg gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac
     4381 tgcttcctgt ccgtaacgaa atgggggcat tcggcgattg tgaataccag cggtaacggc
     4441 gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa gcacgttgct
     4501 gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc
     4561 catgctaact cgtccaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag
     4621 cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca tctggtggaa
     4681 ggcaatcaga gcctcgagag cggggagccg ctggcctacg gtaagagcat caccgatgcc
     4741 tgcatcggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc agtaaaagcg
     4801 cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc gatgaatcac
     4861 cacaggcctg ataagtcgcg cagcgtcgca tcaggcaatg tgctccattg ttagcaacaa
     4921 aaatgccgac tcacttgcag tcggctttct cattttaaac gaatgacgtt tacttcgctt
     4981 taccctggtt tgcaaccgcc gctgctttcg ctgcgatctc gtcagcatta cccagataat
     5041 agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg ccagtcggga
     5101 tattaagctc aagaatctct tcttcgctca tgttatcaag atatttcacc agcgcacgta
     5161 aagagttacc gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc ggcagaatag
     5221 tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc gtcagcggca
     5281 gttctttttc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc tcatcatctt
     5341 tagtcagttc cggcggagtc actgcaaaac cacgacgcca ctgtttcacc tgctcgtcgc
     5401 catacttttc agcagtttcc gctttgttca gaccctgcaa cgcaccgtag tgacgttcgt
     5461 tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc agcacattcc
     5521 acagggtatg gatagcgcgt ttcagcacag aggtgtaagc aaagtcaaag ctgtaacctt
     5581 cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca gacagatcca
     5641 cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca tgacgaacca
     5701 gaaccagctt agttacagcc atatacttac tcctcaaatc attttttaat gataataatt
     5761 ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata gcgaaaatag
     5821 tgtcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt ttgctggtga
     5881 tttgagcaat ataaggataa agccctcatt acgagggctt caggtgctac tcagcaataa
     5941 actgatattc cgtcaggctg gaatactctt cgccaggacg caggaagcag tccggttgcg
     6001 gccattcagg gtggtttggg ctgtccggca gaaactcgct ttccagtgcc aggccttgcc
     6061 agtcggcgta aggttcagtt cctcttgatg gcgtgccgcc gaggaagttg ccggagtaga
     6121 attgcagagc cggagcggtg gtgtagacct tcagctgcaa tttttcatct gctgaccaga
     6181 catgcgccgc cactttcttg ccatcgcctt tggcctgtaa caagaatgcg tgatcgtaac
     6241 ctttcacttt gcgctgatcg tcgtcggcaa gaaactcact ggcgatgatt ttggcgctac
     6301 ggaaatcaaa agacgttccg gcgacagatt tcaggccgtc gtgcggaatg ccgccttcat
     6361 caaccggcag atattcgtcc gccagaatct gcaacttgtg attgcgcacg tcagactgct
     6421 cgccgtcaag attgaaatag acgtgattag tcatattcac cgggcaaggt ttatcaactg
     6481 tggcgcgata agtaatagag atacggttat cgtcggtcag acgatattgc accgtcgcgc
     6541 cgagattacc cgggaagccc tgatcgccat catctgaact cagggcaaac agcacctgac
     6601 gatcgttctg gttcacaatc tgccagcgac gtttgtcgaa cccttccggg ccgccgtgca
     6661 gctggttaac gccctgactt ggcgaaagcg tcacggtttc accgtcaaag gtataacggc
     6721 tattggcgat gcggttggca taacgaccaa tagaggcccc cagaaacgca gcctgatcct
     6781 gatagcattc cgggctggca cagccgagca gcgcctcgcg gacgctgcca tcggaaagcg
     6841 gaatacgggc ggaaagtaaa gtcgcacccc agtccatcag cgtgactacc atccctgcgt
     6901 tgttacgcaa agttaacagt cggtacggct gaccatcggg tgccagtgcg ggagtttcgt
     6961 tcagcactgt cctgctcctt gtgatggttt acaaacgtaa aaagtctctt taatacctgt
     7021 ttttgcttca tattgttcag cgacagcttg ctgtacggca ggcaccagtt cttccgggat
     7081 caacgcgacg atacagccac caaatccgcc gccggtcatg cgtacgccac ctttgtcacc
     7141 aatcacagct ttgacgattt ctaccagagt gtcaatttgc ggcacggtga tttcgaaatc
     7201 atcgcgcata gaggcatgag actccgccat taactcgccc atacgtttca ggtcgccttg
     7261 ctccagcgcg ctggcagctt caacggtgcg ggcgttttcg gtcaggatat gacgcacgcg
     7321 tttcgccacg atcgggtcca gttcatgcgc aacagcgttg aactcttcaa tggtgacatc
     7381 gcgcagagct ggctgctgga agaaacgcgc accggtttcg cactgctcac ggcgagtatt
     7441 gtactcactg ccaaccaggg tacgtttgaa gttactgttg atgatgacca cagctacgcc
     7501 tttcggcatg gaaactgctt tggtccccag tgagcggcag tcaatcagca aggcatgatc
     7561 tttcttgccg agcgcggaaa ttagctgatc catgatcccg cagttacagc caacaaactg
     7621 gttttctgct tcctgaccat taagcgcgat ttgtgcgccg tccagcggca gatgataaag
     7681 ctgctgcaat acggttccga ccgcgacttc cagtgaagcg gaagaactta acccggcccc
     7741 ctgcggcaca ttgccgctga tcaccatgtc cacaccgccg aagctgttgt tacgcagttg
     7801 cagatgtttc accacgccac gaacgtagtt cgcccattga tagttttcat gcgcgacaat
     7861 gggcgcatcg agggaaaact cgtcgagctg attttcataa tcggctgcca ttacgcgaac
     7921 tttacggtca tcgcgtggcg cacagctgat cacggtttga taatcaatcg cgcagggcag
     7981 aacgaaaccg tcgttgtagt cggtgtgttc accaatcaaa ttcacgcggc caggcgcctg
     8041 aatggtgtga gtggcagggt agccaaatgc gttggcaaac agagattgtg ttttttcttt
     8101 cagactcatt tcttacactc cggattcgcg aaaatggata tcgctgactg cgcgcaaacg
     8161 ctctgctgcc tgttctgcgg tcaggtctcg ctgggtctct gccagcattt cataaccaac
     8221 cataaattta cgtacggtgg cggagcgcaa cagaggcgga taaaagtgcg cgtgcagctg
     8281 ccagtgttga ttctcttcgc cattaaatgg cgcgccgtgc cagcccatag agtaggggaa
     8341 ggagcactgg aagaggttgt cataacgact ggtcagcttt ttcaacgcca gtgctaaatc
     8401 gctgcgctgg gcatcggtca aatcggtgat ccgtaaaacg tgggctttgg gcagcagtag
     8461 cgtttcgaac ggccaggcag cccagtaagg cactacggct aaccagtgtt cggtttcgac
     8521 aacggtacgg ctaccgtctg ccagctcgcg ctgaacataa tccaccagca ttggcgattt
     8581 ctgcccggca aaatattctt tttgcaggcg gtcttcgcgc tcagcttcgt taggcaggaa
     8641 gctatttgcc caaacctgtc cgtgcggatg cgggttagag cagcccatcg ccgcaccttt
     8701 gttttcaaag acctgcaccc acgggtacgt ttttcccagc tctgcggttt gctcctgcca
     8761 ggttttgacg atttccgtca atgccgcaac gctcagttct ggcaacgttt tactgtgatc
     8821 cggtgaaaag cagatcaccc ggctggtacc gcgcgcgctc tggcaacgca tcaaaggatc
     8881 gttgctttct ggcgcatctg gcgtgtcaga catcaaggcc gcaaaatcat tagtgaaaac
     8941 gtaagtcccg gtgtaatcgg ggtttttatc gcctgtcacc cgcacattac ctgcgcagag
     9001 gaagcaatct ggatcgtgcg caggtaacac ctgtttggct ggcgtttcct gcgccccctg
     9061 ccaggggcgc ttagcgcggt gcggtgaaac cagaatccat tgcccggtga gcgggttgta
     9121 gcggcgatgt ggatgatcaa cgggattaaa ttgcgtcatg gtcgttcctt aatcgggata
     9181 tccctgtgga tggcgtgact gccagtgcca ggtgtcctgc gccatttcat cgagtgtgcg
     9241 cgttacgcgc cagttcagtt cgcgatcggc tttgctggcg tccgcccagt aggccggaag
     9301 gtcgccctcg cgacgcggtg caaaatgata gttaaccggt ttgccgcagg ctttgctgaa
     9361 ggcattaacc acgtccagca cgctgctgcc tacgccagcg ccgaggttgt agatgtgtac
     9421 gcctggcttg ttcgccagtt tttccatcgc cacgacgtga ccgtccgcca gatccattac
     9481 gtggatgtaa tcgcgtacgc cagtaccatc ttcggtcgga taatcgttac caaaaatcgc
     9541 cagcgagtcg cgacggccta cagcaacctg ggcgatgtat ggcatcaggt tattcggaat
     9601 gccttgcgga tcttcaccca tatcgcccga cggatgcgcg ccaaccgggt tgaagtagcg
     9661 cagcagggca atgctccagt ccggctgggc tttttgcagg tcggtgagga tctgttccac
     9721 catcaatttg cttttgccat aagggctttg cggtgtgccg gtcgggaagc tttcaacgta
     9781 tggaattttg ggctgatcgc cataaacggt ggcggaggag ctaaaaataa agtttttgac
     9841 gttagcggcg cgcatggcgc taatcaggcg cagagtgccg ttgacattgt tgtcgtaata
     9901 ttccagcggt ttttgtaccg attcgccaac ggctttcagc ccggcgaagt ggatcacggt
     9961 gtcgatagcg tgatcgtgca ggatctcggt catcaacgct tcgttacgaa tatcgccttc
    10021 aacaaacgtt ggatgtttgc cgcctaaacg ctcgataaca ggcagtacgc tgcgcttact
    10081 gttacagagg ttatcaagaa tgatgacatc atgaccgttt tgcagtaatt gcacacaggt
    10141 atgacttcca atgtaaccgc taccaccggt aaccagaact ctcataattc gctccattag
    10201 gcttatggta tgaaataacc atagcataac aaagatgcga aaagtgtgac atggaataaa
    10261 ttagtggaat cgtttacaca agaatttagc cgttttttat gcgcgattaa gtgattataa
    10321 aacagagggt ttatgaatga ttgcgctttt tatctgaaaa aagacgcggt ttcatgcctg
    10381 catgcgtcga actgtaggcc ggagaggggg cttacgccac atccggcaca gtcagcatta
    10441 ccaactcagt ttatttttgt cagcgcatag cgataaaggt caccgtccgg cacgaactca
    10501 agacgatggg taatacaggc aggcgcatct tcagcgtggt gcgaaacaaa caacaattgc
    10561 gtttcacctt cgctaatcag cacatcaaca aaacggcgga taagttggcg attgagcgga
    10621 tcaagcccct gtagtggttc atcgagaata agcaatgtcg gatgtttcac cagcgcgcgg
    10681 acgatcagcg ccagacgctg ctgtccccag gaaagactat ggaacggaac gtcagccgtg
    10741 cgtttatcaa tgccgagaat atccagccac tgctgcacca gtttttgctg gcgatccgaa
    10801 acggcctgat aaatgccaat cgaatcaaaa tagccagaaa gaatcacatt acgcacggta
    10861 gtgctgaccc ggtaatccag atgcaaacta ctgctgacgt aaccgatatg ctttttgata
    10921 tcccagatgg tttcgccgct gccgcgacgt cggccgaaaa gcgtcaaatc gttgctgtaa
    10981 ccttgcggat gatcgccagt aatcaggctt aataacgtcg attttcccgc accatttggc
    11041 ccgacaattt gccagtgctc gccaggattc acctgccagc taaggttatt aagaatgggg
    11101 cgatcgttat aagaaaccac gccattattc agcacaatgc gcggttcgtt ggtgggtaag
    11161 gcgtgacgtg ctgaaggttc atccggctcc ggcagttgca caccttcaag ctgttcgcta
    11221 tgcgccagtt gcgcgacgag tgcttgctgg agcagttcct ctttagcgcc cgtttccgct
    11281 aacgtacaat ccgccagcac gccagcaaac tggacaaatt ccgggatctc atcgaagcga
    11341 ttgagcacca gtaccagagt aataccggac tgatgtaacg aggcgagtcg ctcagccagc
    11401 tgctgacgtg aggcaacatc caggccatcg aacggctcat caagaatcaa caagtcaggc
    11461 tccgacatca gcgcctgaca cagcagggtt tttcgcgtct cgccagtgga aaggtattta
    11521 aagcgtcggt cgaggagggc ggtaataccg aactgctgcg ccagttgcat gcaacgcggt
    11581 gcatccttaa cttcatcctg aatgatctca gccgtagtgc gtccggtgtc atcttcgcca
    11641 gggccgagca tatcggtgtt attccgctgc cattcgtcgc tgacgagctt ttgcaattgc
    11701 tcgaaggaga gacgagtgat gtgggaaaac tggctttgcc gttcaccttt caaaagcgga
    11761 agttcccccg ccagcgcacg ggccagcgcc gactttccac ttccattcga accgacaaac
    11821 gcccaactat cacccgcgtt taacgttagc tgaggcaatt gcagcgtttt tgtgtcgcta
    11881 agacgaaacg tgccttgcaa aatttgcaac gatgacattt tatatcccat tttgtgcagc
    11941 gattactgat agggatacgt gtttcataac aaattgtcaa cacgcttagc atagcgtggc
    12001 gataatcacg ctgtcggcat taaagtaggc cgtgacattc tgtccttgct gaagagaagt
    12061 cgcttcatta accggcactg tggcgcacag tgtttgcccg tcgggtagcg ccattaatac
    12121 ttcgcactgc tctgcgccgc gctcaatatg actaataata cccggtaatt ggttgtcggc
    12181 gttttgtgcg accgcttcgt cctgagtaat gcctacccac ggcgctttta gcaatatcaa
    12241 cacttctttg ccttcatcca gccccagacg tgcgccgctt tgtgcggtaa ttgcgacttt
    12301 caggcgtgtt tttccgtcag ccagtaagac atcaacatgc tgttgaacgt catcatgatc
    12361 gcgggcggtg atggtgccga accactggtt acgggcgctg gtttgcagtg aaaaacgtga
    12421 gatcgcggcc agcaggctgt tcagcggcag ggcgtcatcg tcacttaaca catcaaaggc
    12481 tttttgctgg atttgcgcca gtaagtcata gagctgaatc agtcgctgac catagcgggt
    12541 cagtactgcg ccgccgccac ctttaccgcc tgttgcgcgc tcgaccagaa tatgctcact
    12601 taactgattc atctcgttaa tggcatccca ggcgctttta tagctgatac cggcatcttt
    12661 cgctccctgg ctaatggaac cggaaagcgc aatgtgtttt agtagcgaaa tgcggcgcgg
    12721 gtcggcgaat aatttttgtt ggagcttaag ggtgagaagg atttcggcct gcataacaat
    12781 gtcctggcaa aagtcttatt gtgacggaaa acgaacgcca cgcaaagctg accgcacaaa
    12841 aggggagtgc ttttctgtgc ttagcggtta gaatagtctc atgactatat ctggagttga
    12901 ccatgttaga gttattaaaa agtctggtat tcgccgtaat catggtacct gtcgtgatgg
    12961 ccatcatcct gggtctgatt tacggtcttg gtgaagtatt caacatcttt tctggtgttg
    13021 gtaaaaaaga ccagcccgga caaaatcatt gattccctga atgcccgctt agtcgggcat
    13081 tttctttttc tcaacttcct gcttttcctg ccgatatttt ttcttatcta cctcacaaag
    13141 gttagtaata actgctggga aaattccgag ttagtcgtta tattgtcgcc tacataacgt
    13201 tacattaagg ggttaccaat ggctcgtaaa tggttgaact tgtttgccgg ggcggcactc
    13261 tctttcgctg ttgctggcaa tgcactggca gatgaaggga aaatcacggt gttcgccgcc
    13321 gcatcactga ctaacgcaat gcaggacatt gctacgcagt ataaaaaaga gaaaggcgtg
    13381 gatgtggttt cttctttcgc ttcgtcatct actctcgccc gtcagattga agcgggtgcg
    13441 cctgcggatc tgtttatttc tgccgatcag aaatggatgg attatgcagt tgataaaaaa
    13501 gcgatcgata cagctacgcg tcagacactg ctcggcaata gcctggtcgt tgtagcaccg
    13561 aaagccagcg agcagaaaga tttcatcatc gacagcaaaa ctaactggac ttcgctgctg
    13621 aatggcggtc gcctcgcggt tggcgatccg gaacatgtcc ctgctggcat ttatgcaaaa
    13681 gaagcactgc aaaaactggg cgcatgggat acgctctccc cgaaactggc tccggcggaa
    13741 gatgttcgtg gcgcgctggc gttggtcgaa cgcaatgaag cgccgttggg cattgtttac
    13801 ggttctgacg cagttgccag caaaggggta aaagtggttg ccaccttccc ggaagattca
    13861 cataaaaaag tggaatatcc ggttgctgtt gtggaagggc ataacaatgc gacagtgaaa
    13921 gcattttatg attatctgaa gggaccgcag gctgcggaaa tctttaaacg ttacggattt
    13981 acaaccaagt aatgatactg accgatccag aatggcaggc agttttatta agcctgaaag
    14041 tttcttccct ggctgtgctg tttagcctgc cgtttgggat cttttttgcc tggttactgg
    14101 tgcgttgcac gtttccgggc aaagctttgc tcgacagcat actgcatcta ccgctggtgt
    14161 taccgcccgt ggtcgtcggt tacttattat tagtttcgat gggacggcgc ggatttatcg
    14221 gtgaacgtct gtatgactgg tttggtatta ccttcgcctt tagctggcgc ggcgcggttc
    14281 tcgctgccgc cgtcatgtcg tttccgctaa tggtgcgggc aattcgtctg gcgctggaag
    14341 gggttgatgt caaactggaa caggccgcaa gaacactggg ggccgggcgc tggcgcgttt
    14401 tctttactat cacgttaccg ctgaccttac cgggaattat tgttggtacg gtactggctt
    14461 ttgctcgttc tctcggtgaa tttggcgcaa ccatcacctt tgtgtcgaac attcctggtg
    14521 aaacgcgaac cattccttct gccatgtata ccctgattca gacccccggc ggggaaagtg
    14581 gcgcagcgag actgtgcatt atttctattg cgctggcgat gatctccctg ttgatttcag
    14641 aatggctggc cagaatcagc cgtgaacggg cggggcgcta atcatgctgg aactgaattt
    14701 ttcccagacc ttgggcaacc attgcctgac tattaatgaa acgctgcccg ccaatggcat
    14761 cactgctatc tttggcgtct ccggtgccgg aaaaacttcg ctgattaacg ccatcagtgg
    14821 actgacgcgc ccgcaaaaag ggaggattgt cctcaatggt cgggtactaa atgatgccga
    14881 aaaaggtatc tgcctgacgc cagaaaaacg tcgcgttggc tatgtttttc aggatgcacg
    14941 gctgttcccg cattacaaag tgcgtggcaa tttgcgctac ggcatggcga aaagcatggt
    15001 cgatcagttc gataagctgg tggcgctttt aggcattgaa ccgttgcttg accgtttacc
    15061 gggcagcctg tccggaggcg aaaaacagcg cgtggcgatt ggtcgggctt tgctgacggc
    15121 tccggaattg ctgttgctgg atgaaccgct ggcgtcactg gatattccgc gtaaacgcga
    15181 actgttgcct tatctgcaac gtctgacgcg ggaaatcaac attccgatgt tgtatgtcag
    15241 ccattcgctg gatgagatcc tccatctggc agacagagtg atggtactgg aaaacggtca
    15301 ggtgaaagcc tttggcgcgc tggaagaagt ctggggtagt agcgtgatga atccgtggct
    15361 gccgaaagag caacaaagta gcattctgaa agtgacggta cttgagcatc atccgcatta
    15421 cgcgatgact gcgctggcgc tgggcgatca gcatttgtgg gtcaataagc tggacgaacc
    15481 gctgcaagct gcgctacgta tccgcattca ggcttccgat gtttctctgg ttttacaacc
    15541 gccgcagcaa accagcattc gtaacgtatt gcgggcaaaa gttgttaata gttatgacga
    15601 caacggccag gtggaagtgg agctggaagt cggcggtaaa acgctgtggg cgcgtatcag
    15661 cccgtgggcc agggatgaac tggcgatcaa acctggcctg tggctgtacg cgcaaattaa
    15721 aagtgtgtcg ataaccgcct gattaaatca ggtggctata aatgaactgg gcaatgctgt
    15781 cggtggtgtt atcaccaatc acaatgttgg cgcgcgcttt taccgcgtca tcggcgttgc
    15841 ccatcgccac gcctgtacca gcggcttcca gcatactgat atcattaaag ttatcgccga
    15901 atgccacgac gttttccatc gaccaacctt gcgcctcaac ccatttcgtc aaacgtttac
    15961 ctttgctgtt gccgccgcgt gcaatatcaa cctgatcgtg ccaggaccat tcacactcca
    16021 gtcccagttc atgttcgaca tgcttaccaa aatgctgcaa ttgcggcagg tcatcgtgcg
    16081 tcagggcgaa cttccatacg gcgttaactt gttgcgccgt ttcagccaga gaagcgactt
    16141 gtgtgaaagt cggacgctgt tccggcggca gggtttgcgc ccagttagac gtgcgaatga
    16201 catgcccggt cgggtgctca tagaccattg catcatcgac atacatcaga ccgtgaatgt
    16261 ggtgttcatt cagcatctca atgagttgca gggctttatt aacgggcatt gggtccgctt
    16321 ccagcacggt ttttgcatga taatcataca aataggtgcc attacagcaa atagcaggtg
    16381 tatccagcgc cagcgcctga taaaaaggat gaatagcgac gtgatggcga cctgtgacga
    16441 tgattaattg atagcctgct tcgcgagcgc gggccagggc ttctatcgat gaaggaagca
    16501 gggtcttttt cggggtcaat aaggtgccgt ctaagtcgag agcaatcacg cgtgtggtca
    16561 tggcgtattc cagattaagg ttaagaattt tcgtctgcgc gaatggtaca ccgataccac
    16621 tatcggacaa aattctgcgt tttaattcag cattcaccgc caaaagcgac taattttagc
    16681 tgttacagtc agttgctaaa tgcaaaggag cattcatgaa gcaaacagtt tatatcgcca
    16741 gccctgagag ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga
    16801 cacaggttgt cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt
    16861 atctctatgt tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg
    16921 atggcgcact gacctttgcc gcagagtctg cgctgccggg tagtccgacg catatttcca
    16981 ccgatcacca ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa
    17041 cgcgtctgga agatggcctg ccagtgggcg tcgtcgatgt ggtcgagggg ctggacggtt
    17101 gccattccgc caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg
    17161 atcgcatttg cctgtttacg gtcagcgatg atggtcatct cgtggcgcag gaccctgcgg
    17221 aagtgaccac cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat
    17281 atgcgtattg catcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc
    17341 acggtaatat cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc
    17401 gttgggcggc tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta
    17461 ccgccagcct gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag
    17521 gcttccagcc aacggaaacc cagccgcgcg gcttcaatgt tgatcacagc ggcaagtatc
    17581 tgattgccgc cgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg
    17641 gactactgca tgaaaaaggc cgttatgcgg tcgggcaggg accaatgtgg gtggtggtta
    17701 acgcacacta accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat
    17761 ctcctgtaac gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc
    17821 agtccacaaa ttaatcagca aggcaaaaaa gcgttcagct gccggagaaa gcacggcatc
    17881 tttgcggcga ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat
    17941 aatcgaagaa tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc
    18001 tgcctccact agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt
    18061 ccagggcttg tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac
    18121 gccaatcatt ttgtaaccca ctaactcctg ccattctaca agctgctttt tggccaatgg
    18181 atggtcacgc cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga
    18241 tgaatttgtg acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc
    18301 catgcaatta ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt
    18361 aaatttaccg attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaacgt
    18421 caccatacca cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt
    18481 ctttattaat tcccgcgcct ccgggagcaa ccttttccct gcttttgtta acgtaacctt
    18541 gcgagttgta cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg
    18601 cgttaatgca ggttgcgtaa tattgagtga tttagcggca ttgttaaatg aactggactc
    18661 tgccagtatg acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa
    18721 tggaagcgac gattttgggt ggctggccgt taaaaatttt aactgcattt agccaactta
    18781 aattaatgaa aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgccctta
    18841 atgtaaccat atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata
    18901 ccctgcgtga tgatgcgagg tggaacctcg aggggcgcgt tcttgttagc ggaacattta
    18961 cccgaagatc aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat
    19021 ctggaaattg acggtattgg cggcggtaat ccgctgacca gtaaagtcgc cattattagc
    19081 cgttccagcg atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat tgtccatgag
    19141 caacgtgtcg ataccacgcc taattgtggc aatatgctgt ctggtgttgg ggcattcgcc
    19201 attgaaaatg gtttgattgc agcgacttca ccagttaccc gcgtacgtat ccgcaacgtc
    19261 aatactggta cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag
    19321 ggtagcgcca gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg
    19381 aatgccgccg gaaccaaaac cggaaaagtc ttcccgactg ataatcagat tgattatttt
    19441 gacgatgtcc cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa
    19501 tatctgggaa aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta
    19561 gcacgcattg aatctatccg tctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt
    19621 aatatggtta tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat
    19681 gtacgttatt ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct
    19741 atttccagta gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga
    19801 tacggcaata tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa
    19861 ggtcaggatg ccacgacgtt acgcgcatct gttattcgga cgaccagaaa aatattttcc
    19921 ggtgaagttt atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac
    19981 ttctgtatga gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag
    20041 ctaattctga tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta
    20101 agcgaactgg cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc
    20161 aagcctttcc cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc
    20221 ggtaacttat ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt
    20281 actacctggc tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta
    20341 ggtaaacgta ttgcctatct gctgattggt aaaatcggta acaccacgct gggtctgggt
    20401 tacgttacgg tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt
    20461 gcgggcggca ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg
    20521 gaaaaaagtc cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa
    20581 accaccagct atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc
    20641 aacgacattc tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg
    20701 ggcatcatta tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt
    20761 aaaaaggtgg ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa
    20821 atccgcgaaa aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt
    20881 aagtctctgg gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg
    20941 ctgggtatcg ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc
    21001 tggtacggcg gtattatcgg attaagctcc ttattatcga aagttaaatt cttcgaatgg
    21061 ttagctgaag tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc
    21121 gttattattt tcctcagcat tatcgtgcgt tatttcttcg cttccggtag tgcctatatc
    21181 gttgctatgt taccggtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta
    21241 accgcgctgg cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc
    21301 gcggcaggtc cggtcatctt tggcgtgggt tataacgata ttaaatcctg gtggttggtc
    21361 ggtgcggtac tgacgatatt aaccttcctg gtgcatatca ccctcggcgt gtggtggtgg
    21421 aatatgctga tcggctggaa catgctgtaa atattctcgt catacttcaa gttgcatgtg
    21481 ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg gattcactcg
    21541 cttgtcgcct tcctgcaact caaattattt agagtatatc catttattat ctttctgcgc
    21601 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
    21661 ttctcgccag taataacgaa ataattgctg aagaacattt caccggcgaa attaaaaaag
    21721 aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat acgtccggaa
    21781 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
    21841 gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga
    21901 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
    21961 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
    22021 tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca
    22081 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggt ggtgagttgg
    22141 taaaacagct gcttaatgac acctgggata tcgactatcc gggagttgtt gcggtgcatc
    22201 tgaccggaaa accagcgccg tatgtggggc cgcaggatgt ggcgctggct atcattggcg
    22261 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
    22321 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
    22381 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
    22441 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
    22501 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
    22561 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
    22621 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
    22681 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
    22741 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt
    22801 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
    22861 ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata
    22921 cgccaatcaa caacggatta agtattcgcc acaccacgcg taacttcccg aaccgcgaag
    22981 gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
    23041 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
    23101 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
    23161 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
    23221 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga
    23281 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
    23341 gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga
    23401 ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg
    23461 cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca
    23521 gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc
    23581 agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt
    23641 ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag
    23701 tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt
    23761 ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa
    23821 gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa
    23881 accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat
    23941 gcgatgcttg cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccggataagg
    24001 cgtttacgcc gcatccggca aatagttaat agctcttact tcttcgcctc tgcaaccact
    24061 ttactaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
    24121 ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa cgcacgatta
    24181 gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat ggcgctatca
    24241 cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc
    24301 acaccatcac cgaaagcatt gaaacggctg tttacggcga ggaaaccgta gtaaatgttg
    24361 gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt caccacgcgg
    24421 aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
    24481 atgtagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg gttctgcaca
    24541 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt
    24601 acctggtcac catcagtacg cagtgccacc gccggatggt tacctgcatc tacgctatcg
    24661 cccagcgtgt tttcgatggt cagattttgc agttgcaggc cattgttttg tgaccagaag
    24721 accgcagagc agagaacacc gatactgtcg ctgcgtttgc tctggcagct atcgtacata
    24781 taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
    24841 gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
    24901 gtaccgtaca gagtaattcc acccggagca gcagggacat acaccgttcc ctgatactca
    24961 ccaggcatca cggcaatata ctggcgcttg ttagtgcgct tgataattgc cgcatctacc
    25021 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
    25081 ggttgcgcag gcagggtaat cggggaagga ttccacgctg cagcacctgg tgtcagggat
    25141 gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa
    25201 gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gtggtgttga gctacaggcg
    25261 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
    25321 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
    25381 gttggcatta taaaaaaaca ttgcttatca atttgttgca acgaacaggt cactatcagt
    25441 caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat
    25501 actgtgatgc catggtgtcc gacttatgcc cgagaagatg ttgagcaaac ttatcgctta
    25561 tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc
    25621 cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg
    25681 atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc
    25741 atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga
    25801 tggcaatttt tacgcctgtt ttgctttgtt cgacataaag atatccatct acgatatcag
    25861 accacttcat ttcgcataaa ttaccaactc gctgcccggt aacaacagcc agttccattg
    25921 caagtctgag ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag
    25981 ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg
    26041 ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta
    26101 acttggctga cgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt
    26161 ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc
    26221 tcatgtaatt tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc
    26281 gatcaagcca tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct
    26341 tgtgtttgtg tcctgaaaat aactcaatat tggcctgtat tgcttcagtg attgctatcc
    26401 tcctgtctcg gcctaaacca aactctttac ccgtccttgg gtccctgtag cagtaatatc
    26461 cattgtttct tatataaagg tt
//
LOCUS       DAHBHC010000072        26271 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHBHC010000072 DAHBHC010000000
VERSION     DAHBHC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.072"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(41..1405)
                     /gene="rhlE"
                     /locus_tag="M2A35_003583"
     CDS             complement(41..1405)
                     /gene="rhlE"
                     /locus_tag="M2A35_003583"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCB7199829.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1634..2305
                     /gene="cecR"
                     /locus_tag="M2A35_003584"
     CDS             1634..2305
                     /gene="cecR"
                     /locus_tag="M2A35_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCB7199830.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2308..3303
                     /gene="hlyD"
                     /locus_tag="M2A35_003585"
     CDS             2308..3303
                     /gene="hlyD"
                     /locus_tag="M2A35_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCB7199831.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3296..5032
                     /locus_tag="M2A35_003586"
     CDS             3296..5032
                     /locus_tag="M2A35_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7199832.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5025..6158
                     /locus_tag="M2A35_003587"
     CDS             5025..6158
                     /locus_tag="M2A35_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7199833.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6169..7275
                     /locus_tag="M2A35_003588"
     CDS             6169..7275
                     /locus_tag="M2A35_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7199834.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7237..7647)
                     /locus_tag="M2A35_003589"
     CDS             complement(7237..7647)
                     /locus_tag="M2A35_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCB7199835.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7780..8541
                     /locus_tag="M2A35_003590"
     CDS             7780..8541
                     /locus_tag="M2A35_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCB7199836.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8538..9779
                     /gene="clsB"
                     /locus_tag="M2A35_003591"
     CDS             8538..9779
                     /gene="clsB"
                     /locus_tag="M2A35_003591"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCB7199837.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9779..10735
                     /locus_tag="M2A35_003592"
     CDS             9779..10735
                     /locus_tag="M2A35_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCB7199838.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10771..11484)
                     /locus_tag="M2A35_003593"
     CDS             complement(10771..11484)
                     /locus_tag="M2A35_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCB7199839.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11554..12201)
                     /locus_tag="M2A35_003594"
     CDS             complement(11554..12201)
                     /locus_tag="M2A35_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCB7199840.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            complement(12403..13107)
                     /locus_tag="M2A35_003595"
     CDS             complement(12403..13107)
                     /locus_tag="M2A35_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCB7199841.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13244..13696)
                     /gene="moaE"
                     /locus_tag="M2A35_003596"
     CDS             complement(13244..13696)
                     /gene="moaE"
                     /locus_tag="M2A35_003596"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCB7199842.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13698..13943)
                     /gene="moaD"
                     /locus_tag="M2A35_003597"
     CDS             complement(13698..13943)
                     /gene="moaD"
                     /locus_tag="M2A35_003597"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCB7199843.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13936..14421)
                     /gene="moaC"
                     /locus_tag="M2A35_003598"
     CDS             complement(13936..14421)
                     /gene="moaC"
                     /locus_tag="M2A35_003598"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCB7199844.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14424..14936)
                     /gene="moaB"
                     /locus_tag="M2A35_003599"
     CDS             complement(14424..14936)
                     /gene="moaB"
                     /locus_tag="M2A35_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCB7199845.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14958..15947)
                     /gene="moaA"
                     /locus_tag="M2A35_003600"
     CDS             complement(14958..15947)
                     /gene="moaA"
                     /locus_tag="M2A35_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCB7199846.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15935..16079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16344..17252
                     /gene="yvcK"
                     /locus_tag="M2A35_003601"
     CDS             16344..17252
                     /gene="yvcK"
                     /locus_tag="M2A35_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCB7199847.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPSSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(17444..19465)
                     /gene="uvrB"
                     /locus_tag="M2A35_003602"
     CDS             complement(17444..19465)
                     /gene="uvrB"
                     /locus_tag="M2A35_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCB7199848.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELSARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20044..20721)
                     /gene="bioD"
                     /locus_tag="M2A35_003603"
     CDS             complement(20044..20721)
                     /gene="bioD"
                     /locus_tag="M2A35_003603"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCB7199849.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20714..21469)
                     /gene="bioC"
                     /locus_tag="M2A35_003604"
     CDS             complement(20714..21469)
                     /gene="bioC"
                     /locus_tag="M2A35_003604"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCB7199850.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(21456..22610)
                     /gene="bioF"
                     /locus_tag="M2A35_003605"
     CDS             complement(21456..22610)
                     /gene="bioF"
                     /locus_tag="M2A35_003605"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCB7199851.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            complement(22607..23647)
                     /gene="bioB"
                     /locus_tag="M2A35_003606"
     CDS             complement(22607..23647)
                     /gene="bioB"
                     /locus_tag="M2A35_003606"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCB7199852.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23734..25023
                     /gene="bioA"
                     /locus_tag="M2A35_003607"
     CDS             23734..25023
                     /gene="bioA"
                     /locus_tag="M2A35_003607"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCB7199853.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25082..25558
                     /locus_tag="M2A35_003608"
     CDS             25082..25558
                     /locus_tag="M2A35_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCB7199854.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         6244 a         6971 c         6803 g         6253 t
ORIGIN      
        1 gttcatcagc ctgatgccgg gcatagcccg gcataaaaga ttactgcgca gcggtaggtt
       61 tacgcggacg gcggcgacgt tgttgttcgc ctgccggttt ggcatcgccg aggcggcgag
      121 acggtttttc tgcaggtttc gcgcttgcag attttgcgcc accttcccca cggcgtggtt
      181 gctgttgacc gcgaccacca ccttgcccac gaccgccgcc gccacgttgc tggcgaccgt
      241 tctggatcgg ttcggctttg attgacgggt ccggctcata gcccggaatc gcaatgcgcg
      301 ggatctcttt tttcagcagt ttttcgatat cacgcagcag tttgtgttca tcaacacaca
      361 ccagcgacaa cgcttcaccg gtagcagccg cacgaccggt acgcccgata cggtggacat
      421 aatcttccgg tacgtttggc agttcatagt tgacgacgtg cggcaactct tcgatatcca
      481 gaccgcgcgc agcgatatcg gtcgccacca gtacgcgaat gtcacccgat ttaaaatcag
      541 ccagcgcacg ggtacgcgca ccttgcgatt tattcccgtg gatcgccgca ctacggatgc
      601 catctttatt gagctgctca gccagatggt tagcgccgtg tttggtacgg gtaaacacca
      661 gcacctgctg ccagttccct ttaccaatca tgtgcgacag caattcgcgt ttgcgtttct
      721 tatcaacaaa gtgaacgtgc tgggtcacct gatcagacgc ggtattgcgg cgtgccactt
      781 cgatttccag cgggttgtgc aacagttttt ccgccagggc tttaatatcg tcagagaagg
      841 tcgcagagaa cagcaggttc tggcgtttcg ctggcagttt tgttaacacg cgacggatat
      901 cgtggataaa gcccatgtcg agcatgcggt cagcttcatc gaggacgagg atttcaacct
      961 gatccagctt cactgcattt tgatgttcca ggtccagcaa acgtcccggt gttgccacca
     1021 gaacatcaac gccgccgcgc agcttcatca tttgcgggtt aatgctgaca ccgccaaaca
     1081 ccaccagcga acgaatgttc aaatatttgc tgtaatcacg gacgttttcg ccaatctgcg
     1141 cagccagttc acgggtcggg gtaagaatga gcgcacgtac cggacgacgc cctttggcgt
     1201 gcggctggcg agtgatcagg tgttgcaaca gcggcagcgt aaagcccgct gttttaccgg
     1261 taccggtctg ggcgctagcc atcaggtcgc ggccttccag caccgcaggg atcgcctgct
     1321 gctgaatagg ggtgggttca cggtaaccct gctcggcaac ggcgcgcagg atatcagggc
     1381 ttaaacccaa agaatcgaaa gacataacta ctccgaaccg ccccgaccgt taccggtgta
     1441 gttttcaggg agatactgaa aaggaaaatg acaaaaacca caatgtcatg agggagcgga
     1501 gtgtagcagt ttttgtgacg cagcgcataa attatccctg tgactggcgt agctaaaatt
     1561 cttagccata ctggacaact cccatatgga gtcataatct taatcaatca tttgattaag
     1621 aatggactgt gcgatgaata atcctgccat gacaatcaag ggtgaacagg cgaaaaaaca
     1681 gctgattgct gccgcactgg cgcagtttgg tgaatatgga atgaacgcca ccacccgcga
     1741 gatagccgcc caggccgggc agaatatcgc tgccatcacc tactacttcg gttcgaaaga
     1801 agatttgtac ctcgcctgcg cccagtggat tgccgatttt attggcgagc agttccgtcc
     1861 gcacgccgaa gaggccgaac gcttgttcgc acaaccacag cctgatcgcg cagccatccg
     1921 tgaactgatc cttcgcgcct gcaggaacat gattaagctg ctcacccagg atgacaccgt
     1981 caacctcagc aagtttatct cccgtgagca gctctctccc acggcagcct atcacctggt
     2041 gcacgaacag gtgattagcc cgctacacag ccacctgaca cgcctgattg ccgcctggac
     2101 cggctgcgac gccaacgata cccgcatgat cctccatact catgcgttga ttggtgagat
     2161 tctggcgttc cgtcttggca aagaaacaat tctgttacgt accggctgga ccgcgttcga
     2221 tgaagaaaag accgaactga tcaaccagac ggtgacctgt catatcgacc tgattttgca
     2281 aggattatcg caaaggagtt tgtagtgatg aaaaaacctg tcgtgatcgg attggcggta
     2341 gtggtacttg ccgccgtggt tgccggaggc tactggtggt atcaaagccg ccaggataac
     2401 ggcctgacgc tgtatggcaa cgtggatatt cgtacggtaa atcttagttt ccgtgttggg
     2461 gggcgcgttg aatcgctggc ggtggacgaa ggtgatgcta tcaaagcggg ccaggtgctg
     2521 ggcgaactgg atcacaagcc gtatgagatt gccctgatgc aggcgaaagc gggcgtctcg
     2581 gtggcacagg cgcagtatga tctgatgctt gccgggtatc gcgatgaaga aatcgctcag
     2641 gccgccgcag cggtgaaaca ggctcaagcc gcctatgact atgcgcagaa cttctataac
     2701 cgccagcaag gactgtggaa aagccgcacc atttcggcaa atgacctgga aaatgcccgc
     2761 tcctcgcgcg accaggcaca ggcaacgctg aaatcagcac aggataaatt gcgtcagtac
     2821 cgttccggta accgtgaaca ggacatcgct caggcgaaag ccagcctcga acaggcgcag
     2881 gcgcaactgg cgcaggcgga gctgaattta caggactcaa cgttgatagc accgtctgat
     2941 ggcacgctgt taacgcgcgc ggtggagcca ggcacggttc tcaatgaagg tggcacggtg
     3001 tttaccgttt cactaacgcg tccggtgtgg gtacgtgcct atgttgatga acggaatctt
     3061 gaccaggccc agccggggcg caaagtgctg ctttataccg atggtcgccc ggacaagccc
     3121 tatcacggac agattggttt cgtttcgccg actgctgaat ttaccccgaa aaccgtcgaa
     3181 acaccggatc tgcgtaccga cctcgtctat cgcctgcgta ttgtggtgac cgacgccgat
     3241 gatgcgttac gccagggaat gccggtgacg gtgcaattcg gtaacgaggc aggacatgaa
     3301 tgatgccgtt atcacgctga acggcctaga aaaacgcttt ccgggcatgg acaagcccgc
     3361 cgtcgcgccg ctcgattgta ccattcacgc cggttatgtg acggggctgg tggggccgga
     3421 tggtgcaggt aaaaccacgc taatgcggat gctggcggga ttactgaaac ccgacagcgg
     3481 caatgccacg gtgattggct ttgatccaat caaaaacgac ggcgcgctgc acgccgtgct
     3541 cggctatatg ccgcagaaat ttggtctgta tgaagatctc acggtgatgg agaacctcaa
     3601 tctgtacgcg gatttgcgca gcgtcaccgg cgaggcacgt aagcaaactt ttgctcgcct
     3661 gctggagttt acgtctcttg ggccgtttac cggacgcctg gcgggcaagc tctccggtgg
     3721 gatgaaacaa aaactcggtc tggcctgtac cctggtgggc gaaccgaaag tgttgctgct
     3781 cgatgaaccc ggcgtcggcg ttgaccctat ctcacggcgc gaactgtggc agatggtgca
     3841 tgagctggca ggcgaaggga tgttaatcct ctggagtacc tcgtatctcg acgaagccga
     3901 gcagtgccgt gacgtgttac tgatgaacga aggcgagttg ctgtatcagg gggaaccaaa
     3961 agccctgaca caaaccatgg ccggacgcag ttttctgatg accagtccac acgagggcaa
     4021 ccgcaaacta ttgcaacgcg ccttgaaact gccacaggtc agcgacggca tgattcaggg
     4081 gaaatcggta cgtctgatcc tcaaaaaaga ggccacacca gacgatattc gccatgccga
     4141 agggatgccg gaaatcaaca tcaacgaaac tacgccgcgt tttgaagatg cgtttattga
     4201 tttgctgggc ggtgccggaa cctcggaatc gccgctgggt gcaatattgc atacggtaga
     4261 aggcacaccc ggcgagacgg tgatcgaagc gaaagaacta accaagaaat ttggtgattt
     4321 tgccgccacc gatcacgtca actttgccgt taaacgaggg gagatttttg gtctgctcgg
     4381 gccaaacggc gcggggaaat cgaccacctt taagatgatg tgcggcttgc tggtgccgac
     4441 ctccggtcag gcgctggtgc tggggatgga tctgaaagag agttccggta aagcgcgcca
     4501 gcatctcggc tatatggcgc aaaagttttc gctctatggc aacctgacgg tcgaacagaa
     4561 tttacgcttt ttctctggtg tgtatggctt acgcggtcgg gcgcagaacg aaaaaatctc
     4621 ccgtatgagc gaggcatttg gcctgaaaag tattgcctcc cacgccaccg atgaactgcc
     4681 attaggtttt aaacagcggc tggcgctggc ctgttcgctg atgcatgaac cggatattct
     4741 gtttctcgac gaaccgactt ccggcgttga ccccctcacc cgccgtgaat tttggctgca
     4801 catcaacagc atggtagaga aaggcgtcac ggtgatggtc accacccact ttatggatga
     4861 agcggaatat tgcgaccgca tcggcctggt gtaccgcggg aaattaatcg ccagcggcac
     4921 gccggacgat ttgaaagcac agtcggctaa cgatgagcaa cccgatccca ccatggagca
     4981 agcctttatt cagttgatcc acgactggga taaggagcat agcaatgagt aacccgatcc
     5041 tttcctggcg tcgcgtgcgg gcgctgtgcg tcaaagagac gcggcagatc gttcgcgatc
     5101 cgagtagctg gctgattgcg gtagtgatcc cgctgttgct gctgtttatt tttggttacg
     5161 gcattaacct cgactccagc aagctgcggg tcgggatttt actggaacag cgtagcgaag
     5221 cggcgctgga tttcacccac accatgaccg gttcgcccta catcgacgcc accatcagcg
     5281 ataaccgtca ggaactgatc gccaaaatgc aggcggggaa aattcgcggt ctggtggtta
     5341 ttccggtgga ttttgcggaa cagatggagc gcgccaacgc caccgcaccg attcaggtga
     5401 tcaccgacgg cagtgagccg aataccgcta actttgtaca ggggtatgtc gaagggatct
     5461 ggcagatctg gcaaatgcag cgagcggagg acaacgggca gacttttgaa ccgcttattg
     5521 atgtacaaac ccgctactgg tttaacccgg cggcgattag ccagcacttc attatccccg
     5581 gtgcggtgac cattatcatg acggtcatcg gcgcgattct cacctcgctg gtggtggcgc
     5641 gagaatggga acgcggcacc atggaggctc tgctctctac ggagattacc cgcacggaac
     5701 tgctgctgtg taagctgatc ccttattact ttctcgggat gctggcgatg ttgctgtgta
     5761 tgctggtgtc agtgtttatt ctcggcgtgc cgtatcgcgg gtcgctgctg attctgtttt
     5821 ttatctccag cctgttttta ctcagtaccc tggggatggg gctgctgatt tccacgatta
     5881 cccgcaacca gttcaatgcc gctcaggtcg ccctgaacgc cgcttttctg ccgtcgatta
     5941 tgctttccgg ctttattttt cagatcgaca gtatgcccgc ggtgatccgc gcggtgacgt
     6001 acattattcc cgctcgttat ttcgtcagca ccctgcaaag cctgttcctc gccgggaata
     6061 ttccagtggt gctggtggta aacgtgctgt ttttgatcgc ttcggcggtg atgtttatcg
     6121 gcctgacgtg gctgaaaacc aaacgtcggc tggattaggg agaagagcat gtttcatcgc
     6181 ttatggacgt taatccgcaa agagttgcag tcgctgctgc gcgaaccgca aacccgcgcg
     6241 attctgattt tacctgtgct tattcaggtg attctgttcc cgttcgccgc cacgctggaa
     6301 gtgactaacg ccaccatcgc catctacgat gaagataacg gcgagcattc ggtggagctg
     6361 acccaacgtt ttgcccgcgc cagcgccttt actcatgtgc tgctgctgaa aagcccacag
     6421 gagatccgcc caaccatcga cacacaaaag gcgttactac tggtgcgttt cccggctgac
     6481 ttctcgcgca aactggatac cttccagacc gcgccgttgc agttgatcct cgacgggcgt
     6541 aactccaaca gtgcgcaaat tgccgccaac tacctgcaac agatcgtcaa aaattatcag
     6601 caggagctgc tggaaggaaa accgaaacct aacaacagcg agctggtggt acgcaactgg
     6661 tataacccga atcttgacta caaatggttt gtggtgccgt cgctgatcgc catgatcacc
     6721 actatcggcg taatgatcgt cacttcactt tccgtcgccc gcgaacgtga acaaggtacg
     6781 ctcgatcagc tactggtttc gccgctcacc acctggcaga tcttcatcgg caaagccgta
     6841 ccggcgttaa ttgtcgccac cttccaggcc accattgtgc tggcgattgg tatctgggcg
     6901 tatcaaatcc ccttcgccgg atcgctggcg ctgttctact ttacgatggt gatttacggt
     6961 ttatcgctgg tgggattcgg tctgttgatt tcatcactct gctcgacaca acagcaggcg
     7021 tttatcggcg tgtttgtttt tatgatgccc gccattctcc tttccggtta cgtttctccg
     7081 gtggaaaaca tgccagtatg gctgcaaaac ctgacgtgga ttaaccctat tcgccacttt
     7141 acggacatta ccaagcagat ttatttgaag gatgcgagtc tggatattgt gtggaatagt
     7201 ttgtggccgc tactggtgat aacggccacg acagggtcag cggcgtacgc gatgtttaga
     7261 cgtaaggtga tgtaacttct tatctttcgc cagcaaagac actaccgccg ggccggcaag
     7321 gattgccagc cctgcaattg ccagcaaaaa gttgttttcc agtacacggg acaacagcca
     7381 cagcacaatc agcgctgcgc caaaatacca ggtcgtggtc agctcttcca gcacgtctgc
     7441 cacctcacgc cagcgttgct cggggtggcg ctgaaacacc aacatcatca ctaccgtgac
     7501 acaatacagt gcgcataatc ccacgccgac gtgaaccaga gtcttactca tccagcccac
     7561 caccagcagc gccactacca gtaaatgcaa cataatctgc cagcaactta gaccagttgc
     7621 gacacgaaca cgttgttgcc acttcatggc ttctctcctg aagaatttat ctctacttac
     7681 tcagagtacc gaactttacg gaaaattccg taagaccgca cctttttgtg acgccgacta
     7741 cactatttca atgagataag aggcaaagac aggaaaatta tgcccgatca aacacaacaa
     7801 ttttcgttca aggtgctcac catcaacatt cacaaaggtt ttaccgcgtt taaccgacgc
     7861 ttcattttgc cggaacttcg cgacgcggtg cgtaccgtca gcgccgatat tgtttgcctg
     7921 caggaagtga tgggcgcgca cgaagttcat ccgctgcatg tggaaaactg gcccgatacc
     7981 tcgcactacg agtttctcgc cgacacgatg tggagcgatt ttgcctacgg tcgcaatgcc
     8041 gtatacccgg aagggcatca cggcaacgcc gtactgtcgc gttatcccat tgaacattat
     8101 gagaatcgcg atgtttcggt cgatggtgcg gaaaagcgcg gcgtgctcta ctgccgcatt
     8161 gtgccaccga tgaccggaaa agcgattcat gtgatgtgcg tacatctggg cctgcgtgag
     8221 gcgcaccgtc aggcgcagct tgcgatgctc gccgaatggg tgaatgagct accggacggc
     8281 gaaccggtat tggtggcggg tgatttcaat gactggcggc aaaaagctaa tcatccgtta
     8341 aaagtgcagg ccggactgga tgagattttt acccgcgccc acggacgccc ggcgcgcacg
     8401 tttccggtgc aatttcctct actacgactg gacaggatct acgtcaaaaa tgccagcgcc
     8461 agcgcgccaa ccgcgttgcc gctgcggaca tggcgacacc tttctgatca tgccccttta
     8521 agtgcggaga ttcatttatg aaatgtagct ggcgcgaagg caataagatc cagttgctgg
     8581 aaaacggcga gcaatattat cccgcggtgt ttaaggcgat tggcgaggca caagaacgca
     8641 tcattcttga aacgtttatc tggtttgagg atgacgtcgg caaacagctg catgcggcac
     8701 tactggcagc agcgcaacgc ggggttaaag cggaagtctt gctggatggc tacggttcgc
     8761 cggatctcag cgatgagttt gtcaatgaac tgacggcagc tggcgtggta tttcgctact
     8821 acgatccccg ccctcgcctt tttggtatgc gcaccaatgt gtttcgccgg atgcatcgca
     8881 aaattgtggt gatcgacgcg cgtatcgcct ttattggcgg gctgaattac tccgccgagc
     8941 atatgtccag ctacggtcca gaggctaaac aggattacgc ggtacgcctt gaagggccga
     9001 ttgtcgaaga tattctccag tttgagctgg aaaacctgcc tggacagagc gcggcacgac
     9061 gctggtggcg acgtcatcac aaagcggaag agaaccgcca gccgggagaa gcgcaggtat
     9121 tgctggtctg gcgcgataac gaagaacatc gcgatgatat tgaacgtcac tatctgaaaa
     9181 tgctcactca ggcgcggcga gaagtcatta tcgccaacgc ctacttcttc cccggctatc
     9241 gatttttaca cgccttgcgt aaagcggcac ggcgcggggt gcggatcaaa ctgatcattc
     9301 agggcgaacc ggatatgccg attgtcagag tcggtgcgcg cttgctgtat aactatctgg
     9361 ttaaaggcgg cgttcaggta tttgagtacc gccgccgccc gctccacggc aaagtggcat
     9421 tgatggacga tcactgggcg acagtagggt ccagtaatct cgatccgctc agtttgtcac
     9481 tgaatctcga agcaaatgtc atcatccacg atcgtcattt taaccagacg ctgcgcgata
     9541 atctgaacgg cattattgcc gcagattgtc agcaggtcga tgagaccatg ctgccgaaac
     9601 gcacctggtg gaacctgacc aaaagcgtgc tggcgttcca ctttttacgc cacttcccgg
     9661 cgctggttgg ctggcttccg gcacacacac cacgtctggc gcaggttgat ccgcccgcac
     9721 aaccgacaat ggaaacgcag gatcgggtag aaactgaaaa cacgggggta aaaccctgat
     9781 gagtaaatca cacccgcgct ggcgcttagc aaagaagatc ctcacctggc tgttttttat
     9841 cgcggtgatt gtgttgctgg tggtctacgc caaaaaagtg gactgggaag aggtctggaa
     9901 ggtcatccgc gactacaatc gcgttgcgct gcttagtgcg gtcgggctgg tggtcgtcag
     9961 ctatctgatt tacggctgct atgacctgct cgcccgcttt tactgcggtc acaaactggc
    10021 gaagcgccag gtgatgctgg tgtcgtttat ctgctacgcc ttcaacctga cgctcagtac
    10081 ctgggtcggc ggcattggta tgcgctatcg tttgtactcc cgactagggt taccgggcag
    10141 cactattacg cggattttct cgctcagtat taccaccaac tggctgggct atattttact
    10201 ggcagggatt atctttaccg caggcgtggt ggagttgccg gatcactggt atgtcgatca
    10261 aactacgctg cgtattctcg gcattggctt actgatgatt atcgcggttt atttgtggtt
    10321 ttgcgctttc gcgaagcacc gccatatgac catcaaaggg caaaagctgg tgctgccttc
    10381 atggaaattc gccctcgctc agatgctgat ttccagcgtt aactggatgg taatgggggc
    10441 gattatctgg ctgttacttg gtcaaagcgt gaactatttc tttgtactgg gcgtgttact
    10501 ggttagtagt attgctggcg tcatcgtgca tattccggcg gggatcggtg tgctggaagc
    10561 ggtgtttatc gcactactgg ctggggagca tacctcgaaa gggagcatta tcgccgccct
    10621 gctcgcttat cgcgtcctgt attactttat cccgctgctg ctggcgctga tctgttacct
    10681 gctactggaa agccaggcga agaagctacg ggcgaaaaat gaagcggcga tgtgaagttt
    10741 agttaagttc tttagtatct gcatttacga ctaatgaaaa aaacgcgtat gcctttgcca
    10801 gacaagcgtt atagctatcg cggcaagtat cccaaagaag ccgataaaat agtaatagag
    10861 tgttaatgca cctgtaacag cgcaacgaac cggactccac aactcttcac tatggcattt
    10921 ccccgccaat tcaaggagct tatttcgtcc atgcgagata atgcctgacc ataacacaat
    10981 cattaagcaa cttattaccc aaacgggtcg ttcactcatt aatgcggcat tcatgatgat
    11041 caccagggcc aaccctgtca acgtcatcat aatgataaaa cggtgagaac caggatcaac
    11101 attaaataat aaaccggcca gcatactgat taaaaacatc gcgcccgcag tgctgaacac
    11161 gacgataatg gcaggtacag gagctaaact agcaacgaca atcgccacca aagagagtaa
    11221 aaatagatat attacgcctg cgatgacggc atttatcttg ttgatatcct tacttaagcc
    11281 atacatacaa agtaataata atcctatggc gacatacatt tgcggtgaat catattttgt
    11341 tgccgtaatt tcgagatact tcgctaccca ggccagtcct gcggttgtca ccaaacctaa
    11401 agcggtaatg agccatatgc tgtttaatat cctggcttcg tgctgaaaat cattagttat
    11461 gttatttgct gagcgcgaat tcattatttt tatatcctta agttaaatat cattatctat
    11521 tccaattaac gcaataaaac tcgtcaattc gcactaccac actaaaccat taataaattc
    11581 aagaactgtt acagtaattc taaagaggac gcttaaaata ctaaaataga gtgttattgc
    11641 cccaggaacg atacagcgaa gagaacctga aatttcagag gtgtcggcaa cacggatcaa
    11701 atcttccagt gttgttgctt cacaggcagc cgtaatgccc cagataaaga cagtcaaaat
    11761 actcgctaac cacacggaaa aactgctggt aaggatcagg ttaacaacaa aagcaatact
    11821 caaaccacag aaaatatatg aatataactg tctgactggt gggataaccc tattactaca
    11881 agagcaaatc agcgccattg cggcaaacat ccctcccgta attcccatta ccgtcgctat
    11941 gctcgaaaag gtataaaggc tggcgaagag aaagccaaca agaaggatta ttagcagata
    12001 gaccagacat gcgcctgata cattcagtct tttcagggcg ttgtttaacc agagaccacc
    12061 tattatcagc gcagccaaag cgaagacaaa cgcacctgga gacttttgtg acgtaaccca
    12121 atgcaaatgc tgaaatatcc aggcacagcc tgccgtagcc catactccag ctgctgttaa
    12181 ccaccaaatg agaagcagca ttttgctgag cgaagcactt ttttgctgta attgagtagc
    12241 agtggattgt ctgttcaaat gcgttatctg tccctgaaat tacgtagtgg acatagtctg
    12301 gcatttgccg cgattcagtt ctcatgtaaa tgagcggaat agtctttata aaatccgaaa
    12361 aaaatcccgg ctccaggccg ggatcagcag gaagaagaga atttaacggc ggttgccgaa
    12421 gatccgcaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
    12481 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
    12541 cagtttctgc gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
    12601 tgcccacatc aatgcttcgc ttttcaacca gaagttgacc agcgatgcca gcacaatgcc
    12661 gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc
    12721 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
    12781 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
    12841 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
    12901 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
    12961 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
    13021 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
    13081 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
    13141 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgtatgt actgagcggt
    13201 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
    13261 cctgctgatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
    13321 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
    13381 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
    13441 cgatgcggtg aatcacagtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
    13501 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
    13561 tgtcgcccag gttatggttg cgcaccttac cagtaaaggt gactaccgca ccgtcttcgt
    13621 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcgga ccaacaacaa
    13681 ttttggtttc tgccatctta acctccggtt accggcggga agaaagctac ttcgtcgccg
    13741 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag taatttgcca
    13801 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca tgtgctggcg taacgcttca
    13861 acagttggga aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
    13921 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
    13981 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
    14041 ataaatggtc agcgccgcca cggaggccgc ggttaatgct tccatttcga caccggtttt
    14101 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
    14161 attgacttca actttgctga gcatcagcgg atgacagagc gggatcagat cccaggtgcg
    14221 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
    14281 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
    14341 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
    14401 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
    14461 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
    14521 gaacccggca tggcgaaaat cagcgttttg ttggcgacgc ccgctaccgc acgagattgc
    14581 aacgtggaag tgccaatctc ttcaaaagac aacatccgga acacttcacc aaaaccttca
    14641 acttcacggt cgaacaacgg cagcaatgct tcgggagcct gatcaccttc cgtcaggcca
    14701 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
    14761 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
    14821 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
    14881 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
    14941 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
    15001 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
    15061 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
    15121 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
    15181 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
    15241 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
    15301 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
    15361 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
    15421 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
    15481 aaaccggcct caaatgcagc atcaatcccc gccatgacct ggttgaattt atcctgcccg
    15541 gtaatagcgt gaaactggcg ggcgtccaga ctgtcgacac tgacgttaat gccagtaagt
    15601 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
    15661 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
    15721 aaagacggct caccgcctgt caggcgcact ttttcggtgc ccagactggc gaaggcgcgc
    15781 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
    15841 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
    15901 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
    15961 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
    16021 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttatg
    16081 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgt aatcatgtat
    16141 atagcgtcat gatcgtgcta tttcgctata gaataaggga aaatggcggc tttactttga
    16201 tatacatcat cagggaggca tagcgtgcat cgtcctttag aggtagaaga aaatagtttg
    16261 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
    16321 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
    16381 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
    16441 ctcgtttaac gggtatcgtc accaccaccg ataatggtgg ctcgacgggg cgtattcgcc
    16501 gttcagaagg cggcattgcc tggggcgata tgcgcaactg cctcaaccag ctgataacgg
    16561 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg tggcaatggc gaactttccg
    16621 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
    16681 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
    16741 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
    16801 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
    16861 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctagcagtt
    16921 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
    16981 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
    17041 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
    17101 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
    17161 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
    17221 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
    17281 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
    17341 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcaca
    17401 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
    17461 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
    17521 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
    17581 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
    17641 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca
    17701 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
    17761 cggtttcgcc aatcgctttc gccattgatg gggtgatctt atcgccgtag agaatcgctt
    17821 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
    17881 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
    17941 cttcgcgcag taagttgatc cctaccagca cgtcgaactc acccagacgc aagtcgcgga
    18001 taatctccat acgttcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcgccat
    18061 gttcttcgag atattcggta agatcttccg ccatccgctt ggtcagtgtg gtgaccagta
    18121 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
    18181 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
    18241 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
    18301 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
    18361 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
    18421 cgccaatttg tggaatggtg acatgagatt catcgaccac cagcagccca tcggcaggga
    18481 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
    18541 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
    18601 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caatagcact ttgcgtctgg
    18661 cggacagctc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
    18721 agtgcgtttt cgggtagatg gtaaaacgtg gaatggtgga aacaatctgc ccggttagcg
    18781 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
    18841 catccgattc tgccgggaag atatcaatca cctcgccacg aacgcggaaa gtaccacgct
    18901 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
    18961 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
    19021 ccagaccata aatcgcggaa acagacgcca ctacaaccac atcacgtcgc tccagcatcg
    19081 ctttggtggc ggataaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
    19141 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
    19201 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
    19261 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
    19321 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
    19381 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
    19441 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
    19501 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
    19561 atcaccataa tatttctgtt acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
    19621 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
    19681 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgttcag tgactgccat
    19741 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
    19801 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
    19861 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
    19921 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
    19981 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagga
    20041 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
    20101 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
    20161 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
    20221 tccggcgtgt tgtattgcct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
    20281 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
    20341 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
    20401 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgcgc
    20461 gctgatgatg tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
    20521 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
    20581 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
    20641 tgcggcttgt aaaagtgcac aactggcgac agttttcccc acttcggtat ccgttccggt
    20701 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcaggggat
    20761 atcgcccctg tttttgtggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
    20821 ttcgcgggtc gcgcccttca tgaagatgcg tggctccaat gcctttcagc gaacgcatgg
    20881 cactgagcgc atcatcaaac cacagcgtga tgggctgaat atgatgttga taatgcacgc
    20941 cgttcagtga ctgttcgatt gcacctggtg gtaaaaagcg attagcatgc ggacgctcgt
    21001 ccactgcctg ccacgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
    21061 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
    21121 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
    21181 gggattcgat atctcccgcc agataatggt ctgcggcatc cttctggcgt gcctgaacaa
    21241 gcattggcgg cgagagatct agcgccgtca cctgcgcgtg acgctctcgc cagtggcggc
    21301 tcatccagcc tggcccacac cccgcgtcca gtacgtgggt gtatttacgc tgtgggagca
    21361 ttgccagtaa gacgtcagca ctctggcgct gtagatctgc gtgttgctca tagtgcgcgg
    21421 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcatctcc
    21481 agcaaacggt caatatcctg catttcatgg gcggcggtta aggtcaggcg tagccgcgcg
    21541 gtgccagcgg gtacggttgg cgggcgaatc gccgtcaccc agcaaccttg ctggcgcagt
    21601 ttttctgcca gctgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgcta
    21661 cgtgaatccg caagcgtaaa cggcgaaccc tgcactccgg cacgaaaacg cgtaatgagt
    21721 gccgccagtt tttcgcgccg agcgtccccc tcttcactgc gaatgaccgc cagcgacgca
    21781 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
    21841 tgcagcagat aatccgccac cgtactggag caaagcaccg ctgccccgct gacgccaaat
    21901 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
    21961 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
    22021 tgctgttgcg ttacctgctg gatttccgcc agtggcgcac tatcgccgtc catgctgaac
    22081 acgccttctg tcaccaccat ttgctgcccc ggacaggggg aagcaagcaa tcgcgccaaa
    22141 tgagtgacat cgttatgagc aaaacggcga agctgcgacg ggcttaaact ggcagcttcc
    22201 agcaatgagg catggctaag ccggtcggca gcaatacggt cctctttcgc catcatcgcg
    22261 gcaataactg cctgattagc ggcaaaacca gagataaata gcagtgcccg cgaatagccc
    22321 agccactcgg ccagttcttc ttccagcgcc tgatgcgcaa cgctataacc gctgacgtga
    22381 ccggaaccgc cgctaccgac gccaaattgc tccgcaccct gctgccaggc acggataatt
    22441 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    22501 tccgccacca gccagcgtcc ggctccttgc gccactggat aacggcgacg cagggcatcg
    22561 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    22621 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg ttcaagacgt tgctgttgtt
    22681 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    22741 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    22801 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    22861 cagaaaggcg cacgtaagag gttggcatca tgatccgcgc gaccgcaatg gtgcgaataa
    22921 aatcaaaggc atcgacatca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    22981 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    23041 cgcgatcttt taccgtttcg cctaagccca caatgccgcc agaacagact ttgatcccgg
    23101 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgggtg gtgatgatat
    23161 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcattcg
    23221 caaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    23281 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    23341 tccacgccgc gcccatacag aaacgcgtcg atcccgcagc tttcgctttg cgcgccgact
    23401 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    23461 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaaagtgc
    23521 tgacctgcac ctgacgagga tcgaaatgct gtcgatgcac ctgctgcgct tcaaacagca
    23581 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    23641 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag actcgtaaac
    23701 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatat tgcctttgac
    23761 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    23821 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    23881 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    23941 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcactcatgc gccagccatt
    24001 gagctgtgcc gcaaactggt ggcgatgacg ccgcaaccac tggagtgcgt ttttctcgcg
    24061 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    24121 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    24181 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    24241 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgagcgc
    24301 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    24361 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    24421 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    24481 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    24541 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac gctcaccacg
    24601 cgcgaggttg cggaaaccat cagtaacggc gaagccggtt gctttatgca cgggccaact
    24661 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    24721 ggcgaatggc agcagcaggt ggcggctatt gaagtacagc tgcgcgagca acttgccccc
    24781 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    24841 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggtgtctgg
    24901 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    24961 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    25021 taacgggaag tcagcgtgag ggtttctggc tacactttct gcaaacaaga aaggagggtt
    25081 catgaaactc atcagtaacg atctgcgcga tggcgataag ttgccgcatc gtcatgtctt
    25141 taacggcatg ggttacgatg gcgataatat ttcaccgcat ctggcgtggg atgaagttcc
    25201 tgcgggaacg aaaagttttg ttgtcacctg ctacgacccg gatgcgccaa ccggctccgg
    25261 ctggtggcac tgggtagttg ttaacttacc cgctgataca cgcgtattac cgcaagggtt
    25321 tggctctggt ctggtagcaa tgccagacgg cgttttgcag acgcgtaccg attttggtaa
    25381 aaccggatac gatggcgcag caccgccgaa aggcgaaact catcgctaca tttttaccgt
    25441 tcacgcgctg gatgtagaac gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg
    25501 gtttaacgtt catttccact ctctggcaag cgcctcgatt actgcgatgt ttagttaatc
    25561 actctgccag atggcgcaat gccatctggt atcacttaaa ggtattaaaa acaacttttt
    25621 gtctttttac cttcccgttt cgctcaagtt agtataaaaa agttgaatgt gaaacagtaa
    25681 aaaccattaa tatcaacgtc ttataattga ctcagtctaa ataatagact acaaaatacg
    25741 acaaaacaca acatacccag tcactatgaa tcaactattt agatagtatt agtgacctga
    25801 gacagagcat tagcgcaagg tgattttttg tcctcttgcg ctaatttttt gtcatcaaac
    25861 atacagccaa tcagaagagc ataaagctgc caagcattat atgtcttggt tttatgctca
    25921 ttttcattaa aaatatatat aataagacta atatcagtat aaaatattta taacacttag
    25981 agtaaaaatg taatcagctt atacaaccaa caataactta aactataaaa aacaataaca
    26041 gaaacattta taatttattt taagtattca agagataaac tggtaaattt aacaacttaa
    26101 ttttgagatc taagtcacat caatcaagca ttcaaacagc tatatattaa agctgtccac
    26161 atcggatatg tgacactaat agtatcaatg gattgatatt attaatggat ataaactatg
    26221 taataaggat ttattatgaa tgttcaacca aatgtgcaca ctggattaac c
//
LOCUS       DAHBHC010000073        26209 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHBHC010000073 DAHBHC010000000
VERSION     DAHBHC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.073"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="M2A35_003609"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="M2A35_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCB7199855.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="M2A35_003610"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="M2A35_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCB7199856.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="M2A35_003611"
     CDS             complement(941..1408)
                     /locus_tag="M2A35_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCB7199857.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="M2A35_003612"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="M2A35_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCB7199858.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="M2A35_003613"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="M2A35_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCB7199859.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="M2A35_003614"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="M2A35_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCB7199860.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="M2A35_003615"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="M2A35_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCB7199861.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="M2A35_003616"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="M2A35_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCB7199862.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="M2A35_003617"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="M2A35_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCB7199863.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="M2A35_003618"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="M2A35_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCB7199864.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="M2A35_003619"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="M2A35_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCB7199865.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="M2A35_003620"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="M2A35_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCB7199866.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="M2A35_003621"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="M2A35_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCB7199867.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="M2A35_003622"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="M2A35_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCB7199868.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="M2A35_003623"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="M2A35_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCB7199869.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="M2A35_003624"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="M2A35_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCB7199870.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="M2A35_003625"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="M2A35_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCB7199871.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="M2A35_003626"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="M2A35_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCB7199872.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="M2A35_003627"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="M2A35_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCB7199873.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="M2A35_003628"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="M2A35_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCB7199874.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="M2A35_003629"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="M2A35_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCB7199875.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="M2A35_003630"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="M2A35_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCB7199876.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="M2A35_003631"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="M2A35_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCB7199877.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="M2A35_003632"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="M2A35_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCB7199878.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="M2A35_003633"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="M2A35_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCB7199879.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="M2A35_003634"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="M2A35_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCB7199880.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="M2A35_003635"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="M2A35_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCB7199881.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="M2A35_003636"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="M2A35_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCB7199882.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="M2A35_003637"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="M2A35_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCB7199883.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="M2A35_003638"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="M2A35_003638"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCB7199884.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="M2A35_003639"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="M2A35_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCB7199885.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="M2A35_003640"
     CDS             15548..15916
                     /locus_tag="M2A35_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCB7199886.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="M2A35_003641"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="M2A35_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCB7199887.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="M2A35_003642"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="M2A35_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCB7199888.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPSFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="M2A35_003643"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="M2A35_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCB7199889.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKQ
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="M2A35_003644"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="M2A35_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCB7199890.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQMVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="M2A35_003645"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="M2A35_003645"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCB7199891.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMSGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="M2A35_003646"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="M2A35_003646"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCB7199892.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="M2A35_003647"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="M2A35_003647"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCB7199893.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="M2A35_003648"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="M2A35_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCB7199894.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="M2A35_003649"
     CDS             22593..23066
                     /locus_tag="M2A35_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCB7199895.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="M2A35_003650"
     CDS             23095..23637
                     /locus_tag="M2A35_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCB7199896.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="M2A35_003651"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="M2A35_003651"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCB7199897.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="M2A35_003652"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="M2A35_003652"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCB7199898.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="M2A35_003653"
     CDS             25034..25291
                     /locus_tag="M2A35_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCB7199899.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="M2A35_003654"
     CDS             complement(25267..25821)
                     /locus_tag="M2A35_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCB7199900.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6741 a         6213 c         6784 g         6471 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 tgcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatccctcat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattctg tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgctaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggctataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccatct gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccgct catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tttacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcga aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatctt ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgtcacctcg cgacagtgcg ctaaagcgcg tcgcaactgc tctttaacaa
    26161 tttatcagat aatctgtgtg ggcactcgaa gatacggatt cttaacgtc
//
LOCUS       DAHBHC010000074        25283 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHBHC010000074 DAHBHC010000000
VERSION     DAHBHC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.074"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            90..437
                     /gene="grxD"
                     /locus_tag="M2A35_003655"
     CDS             90..437
                     /gene="grxD"
                     /locus_tag="M2A35_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCB7199901.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(488..5104)
                     /locus_tag="M2A35_003656"
     CDS             complement(488..5104)
                     /locus_tag="M2A35_003656"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCB7199902.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAVHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(5197..5844)
                     /gene="rnt"
                     /locus_tag="M2A35_003657"
     CDS             complement(5197..5844)
                     /gene="rnt"
                     /locus_tag="M2A35_003657"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCB7199903.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5947..6354)
                     /gene="gloA"
                     /locus_tag="M2A35_003658"
     CDS             complement(5947..6354)
                     /gene="gloA"
                     /locus_tag="M2A35_003658"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCB7199904.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6435..7532)
                     /gene="nemA"
                     /locus_tag="M2A35_003659"
     CDS             complement(6435..7532)
                     /gene="nemA"
                     /locus_tag="M2A35_003659"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCB7199905.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7569..8168)
                     /gene="nemR"
                     /locus_tag="M2A35_003660"
     CDS             complement(7569..8168)
                     /gene="nemR"
                     /locus_tag="M2A35_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCB7199906.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCELSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8271..8510
                     /locus_tag="M2A35_003661"
     CDS             8271..8510
                     /locus_tag="M2A35_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCB7199907.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8559..9455
                     /locus_tag="M2A35_003662"
     CDS             8559..9455
                     /locus_tag="M2A35_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCB7199908.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9536..10057
                     /gene="sodC"
                     /locus_tag="M2A35_003663"
     CDS             9536..10057
                     /gene="sodC"
                     /locus_tag="M2A35_003663"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCB7199909.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10058..12070)
                     /locus_tag="M2A35_003664"
     CDS             complement(10058..12070)
                     /locus_tag="M2A35_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCB7199910.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(12070..12927)
                     /locus_tag="M2A35_003665"
     CDS             complement(12070..12927)
                     /locus_tag="M2A35_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCB7199911.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12930..13166)
                     /locus_tag="M2A35_003666"
     CDS             complement(12930..13166)
                     /locus_tag="M2A35_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCB7199912.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13367..13801
                     /gene="slyA"
                     /locus_tag="M2A35_003667"
     CDS             13367..13801
                     /gene="slyA"
                     /locus_tag="M2A35_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCB7199913.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13848..14315)
                     /gene="slyB"
                     /locus_tag="M2A35_003668"
     CDS             complement(13848..14315)
                     /gene="slyB"
                     /locus_tag="M2A35_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCB7199914.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14589..15698
                     /gene="anmK"
                     /locus_tag="M2A35_003669"
     CDS             14589..15698
                     /gene="anmK"
                     /locus_tag="M2A35_003669"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCB7199915.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15796..16125
                     /gene="mliC"
                     /locus_tag="M2A35_003670"
     CDS             15796..16125
                     /gene="mliC"
                     /locus_tag="M2A35_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCB7199916.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16184..16840
                     /gene="pdxH"
                     /locus_tag="M2A35_003671"
     CDS             16184..16840
                     /gene="pdxH"
                     /locus_tag="M2A35_003671"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCB7199917.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQLENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16969..18243
                     /gene="tyrS"
                     /locus_tag="M2A35_003672"
     CDS             16969..18243
                     /gene="tyrS"
                     /locus_tag="M2A35_003672"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCB7199918.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18302..19165
                     /gene="pdxY"
                     /locus_tag="M2A35_003673"
     CDS             18302..19165
                     /gene="pdxY"
                     /locus_tag="M2A35_003673"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCB7199919.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19209..19814)
                     /gene="gstA"
                     /locus_tag="M2A35_003674"
     CDS             complement(19209..19814)
                     /gene="gstA"
                     /locus_tag="M2A35_003674"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCB7199920.1"
                     /translation="MKLFYKPGACSLASHITLHESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19920..21422)
                     /gene="dtpA"
                     /locus_tag="M2A35_003675"
     CDS             complement(19920..21422)
                     /gene="dtpA"
                     /locus_tag="M2A35_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCB7199921.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(22033..22668)
                     /gene="nth"
                     /locus_tag="M2A35_003676"
     CDS             complement(22033..22668)
                     /gene="nth"
                     /locus_tag="M2A35_003676"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCB7199922.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTSRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22668..23363)
                     /gene="rsxE"
                     /locus_tag="M2A35_003677"
     CDS             complement(22668..23363)
                     /gene="rsxE"
                     /locus_tag="M2A35_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCB7199923.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23367..23987)
                     /gene="rsxG"
                     /locus_tag="M2A35_003678"
     CDS             complement(23367..23987)
                     /gene="rsxG"
                     /locus_tag="M2A35_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCB7199924.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23991..25049)
                     /gene="rsxD"
                     /locus_tag="M2A35_003679"
     CDS             complement(23991..25049)
                     /gene="rsxD"
                     /locus_tag="M2A35_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCB7199925.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(25050..>25283)
                     /locus_tag="M2A35_003680"
     CDS             complement(25050..>25283)
                     /locus_tag="M2A35_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199926.1"
                     /translation="ARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAE
                     PEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         6261 a         6546 c         6600 g         5876 t
ORIGIN      
        1 aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact
       61 gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc
      121 agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg
      181 gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg
      241 atattctgca gaatccggac attcgtgcgg aactgccgaa atatgctaac tggccgacct
      301 tcccgcaact gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg atcgaaatgt
      361 atcagcgtgg cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag
      421 agccggacgc ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt
      481 cctttctcta tccccaatcc agcccttgtg gcgaacttga aaatcccgcc tcacgcagca
      541 gcgtaaacat cggcgtttgc cggaccggta gatcattcac ttctgttagc gtaaagcgca
      601 gccgtggttc gcgacgcagt gcggtagtca gcgcgtggaa aacctctggg gcgagtaatt
      661 cctctttttc ctgccagacc agcatttttt tgccaccttg cgccagatag agtaacaatt
      721 tgccgccaga aacgaccacc agcgcacccg cccgacgcgt tggaaccagc gttgccggat
      781 gtgcaggcca gggcagaaga tttccccaca cattagccgg atcgttggca gagagcgcca
      841 ctggtgtata gtggcgcgtt tgcgtggctt gtgtcgccag atcgcgcaat cggtcaatag
      901 tcagacgttc agcgaattgc gcgccaccca gaccttctac aaaacgacct cgcataattc
      961 gcccggagtc ttccatactt cgacaaagcg tttgcatcga tggaaacccg ccagggatat
     1021 tttcggctat caccgcctga cgactgatga tgccgtagcg gtcgagcata ttttccgcca
     1081 gcgccagcat cctttcggta tcgtttagtg gctccacctg caataacgac cagcgtccag
     1141 ccagatttgg tgtgttgtaa gataccagcg gcgagacggg ttgcgcatag acaggacgtc
     1201 cacgacgagc ccggtgactg cggcgagttg aggtgcgtgc gttggaactg ctgcgggtga
     1261 gggcgcgtaa cggtgcccaa atgtcgctgg tgatgacgcc ttgccagacc agcgcccata
     1321 acgcctcttg cagggcagag agatcaaccg attcgccgat tttgtcgcgt atccgttggc
     1381 tgatttgttg tgcaaaccag gctcctccgt cagccagaac agcgactatc gcttgttgca
     1441 gcgccgaacg attcgcctga tccgcttcgg cgggagtgaa cgattctgca gcatattcct
     1501 gtagatgcag tgccaccagg ccgtcatctt cacccagttt tttttgcccc gaccagataa
     1561 ccgcaccggt tgccagtaat tcatcgagca tttctgacga atagtcgcgt acgcgggcag
     1621 gcaggatctg gctttcccag agtgaggcgg gtaaaccgac tccggcaagc tgttcgatca
     1681 cccgcatcac gccatctacg ccctcataaa cgcctggcga tgttgaggca aagagcgccg
     1741 ggctaccatc ggtggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
     1801 tggctgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
     1861 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccaga ccctgttcac
     1921 gcagttgctg aagctgctct tcgacaatgg caataccgag actaaactca tgagccagtt
     1981 gttcagccgt gaccagagca tgagcccgga gatagcgcag aaagagatcg cgaagcgggt
     2041 aactgactct atgtaaataa atctctggca atgactctgg tagtcgtacg ccgagggcat
     2101 cacgcagcct ggcggcatca tccatacagg caagacgctc ctgtccgcta atcatcgctg
     2161 ggaagattcg ttttactgca agaagatttt ccagatacga cgcaacctct tcactgctgc
     2221 ctgtatgccg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
     2281 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
     2341 cctggcgaat gacctgcggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
     2401 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc
     2461 tctggtacat aaattccgcg acatagccga acaataaact tgtggcgaaa ggcgagggcg
     2521 tagtggtcgt tacatcggat atttgaattt cgccaccgtt caggcgacgc atcaaacgtt
     2581 ccagtgcggg aagatcataa acatcttgca gacattcgcg tagggtttcg agaatgaccg
     2641 gaaaatccgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
     2701 aaagcggggt gcgatggccc ggagtgcgcc ccggcattaa taatgcccgc gcggcgcatt
     2761 cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc ctcgcggaca atttgcagca
     2821 acttttctgg ttcaaacaaa aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa
     2881 tgcgcgcaac aatgccgtca tcactggcga ccacagacgc gtcagcgccc catagggcat
     2941 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
     3001 gcaaaataat acgccagtcg ccaatttcat cacggcaccg ttcgaggacc agatggcgac
     3061 tccccggaac gatgcccgtc gcgttgcgtt gctcgtcaat caacccctga atattggcga
     3121 tcgtattttc ttctgccagc cacgggggaa tagtcccgga aaagaacgcg ccatccgcca
     3181 gcaaatgaag aaaatcgccg atcatctcgc ctaattcagc cggacgtccg ttaccttcac
     3241 cacgccagaa ggggagccgg gcagaacgac ccggagcagg agtcacaatc acctgatcgc
     3301 gggtgatttg ctggatccgc catgaggtag cgccgagagt gataatgtcg ttcacccgcg
     3361 actcatatac catctcctca tccagttcac ccacccgccg cgaaccggcc tgttcttcac
     3421 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccgccg ctggtaaccg
     3481 ccaacaattg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
     3541 tggggcgaaa agcagaaaaa tcgccagagg gatagcgccc ggaaagcata tccagcgtgg
     3601 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
     3661 attcgtctac ctgtaatgcc tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt
     3721 cgagtggatt atgcggcggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
     3781 tgactgcgga atcgactaag tcacgccggg tacgagggaa aaacagccct ttagatacac
     3841 cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg
     3901 ttgccacctg aatcaccaga tccactgcgc ccatatcaat ccccagttcg agactggagg
     3961 ttgcgaccac gcagcgtaac tcacccgatt tcagcgcctg ctcggtgatt gctcgttgtt
     4021 ctttagagac ggagccgtgg tgtgagcggg caataaaaac gtcgctactt tgtacacggt
     4081 tagaggttgc gccggaggtc gactcgaaat ggaccgcatc aacggcgata gacggggaac
     4141 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagcccac
     4201 gtgaattagt aaagacaatg gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt
     4261 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgccg gtgctgctgg
     4321 cgaccgatga gacatcatcc atattggcga ccggtacgac aattcgtatc tgcggatggc
     4381 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaat gctgccacat
     4441 cgctggctga gcgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
     4501 catcgagccg ctccagactt aacgccagat gcgcaccacg tttactgccc gctaccgcgt
     4561 ggacttcatc aataattacc gtttcgacgc cgcgtagcgt ttcgcgcgcg cgggaggtca
     4621 gcatcagata gagtgattcg ggtgtggtga tcagaatatc cggcggatta cgggtgagtt
     4681 tgctgcgttc ctgtgcaggc gtatcgccag tacggatccc tacgcgaaga ttgacttccg
     4741 tttcgccgcg ccgccgccgt tcatcggcaa tacccttcaa cgggagctgc aagttgcgct
     4801 gaacgtcggt gcccagagct tttatcggtg aaatatagag gatgcgtgag gttttacgct
     4861 tatgcgcctc gcgggtatct tcgccgcctt cgcggaagag ccgatcgagg gcgtagagaa
     4921 atgctgccag cgttttcccg gagccggtcg gtgcaatcac cagcgcatgt tcgcttcgcg
     4981 ccgccacatg ccaggtttgc gactggacag cggtcggctg tttaaaggcg cgaagaaacc
     5041 agtcgcgggt cgccggtgaa aacacgtccg gcaggagcga tgaagggtct ggattatctg
     5101 ccattaacgc tattccccgt gaatatcatg caggatgcgt ggaatagcct cactatagat
     5161 gcctgaatca ttgcatgtca tcaggcatcg actcgattac acctcttcgg cggcaggtag
     5221 cggccagcct cccagacgtt tccagcggtt gacgatttca caaaacagca cagcagtgcg
     5281 ttcggtgtcg tacagcgcgg agtgcgcctg ggtgctgtcg aagtccatgc cagcggtctg
     5341 gcaagccttt gacaatacgg tttgtccgag tgccagcccg gccagtgcag cagtgtcaaa
     5401 agtggcgaaa gggtggaacg ggttacgttt cagtgaggcg cgttctgcgg cggccatcat
     5461 aaagctgtga tcaaaattgg cattgtgcgc caccataatg gcgcggttac agccgctcgc
     5521 tttaatacct ttacgtacaa ctttaaaaat ttcgtgcagc gcctcgtatt cgctgaccgc
     5581 gccgcgatcg ggatcgttcg ggtcaatgcc gttgaaggcg agggcttctg gttgcaaatt
     5641 tgcgccgaca aatggttcga cgtggaaatg taatgtggtg tccggcatca gccagccttg
     5701 ttcatccatt ttcagggtga tggcggcaat ctcaagcagc gcatcggttt tggcgttaaa
     5761 tccggctgtt tcaacatcga tcacaacagg ataaaaacca cgaaaacggt cgcacagacc
     5821 ggtaagttga gcgttatcgg acatctgatt ctcttaatac ggataaattg cagcgcgcat
     5881 tatgacaaat attacttgtc gatgcagtaa agatgcgggc gcgatgagtt cacgcccggc
     5941 aggagattag ttgcccagac cgcgaccggc gtctttctct tcgattaact caattttgta
     6001 accgtccgga tcttccacaa acgcgataac cgtagtaccg ccttttaccg gacccgcttc
     6061 acgggtcacg ttacccccgt tttgacggat tttttcgcac gcttcagcgg cgttatctac
     6121 gctaagcgcg atgtgaccat aagcagtgcc gagttcgtat ttatccacgc cccagttgta
     6181 ggtcagttca atcaccgctt cttcggtttc cgggccgtag ccaacaaacg ccagtgagta
     6241 tttgtattcc gggttttcgc tggtacgcag cagtttcatg cccagcactt tggtataaaa
     6301 atcgatggag cgttgcagat cgccaacgcg cagcatggta tgaagaagac gcatcttttt
     6361 atcctcaaaa tggctaacag attcaaaatg ttgttttagt atagcggcgg ctttacgccg
     6421 ctcgcaatgt tggattacaa cgtcgggtaa tcggtatagc cttccgcgcc gccaccgtag
     6481 aaactttcgg cacgctgtgg gttaagctca gctttgcgct gcaagcgggc gaccagatcc
     6541 gggttcgcaa tccagtcacg accaaatgcc accgcatcaa ttaacccttt gccgatcagc
     6601 gtttcagctt tttctactgt gtatgcacct gcgccgataa tcggaccgtg gaaacgggcg
     6661 cgtacttttt cgcggaacgc atcagtatac ggttcacccc ccgcccaatc tggttctgac
     6721 atatgcagat aagcaatgcc gcgtttaccc agttgttcaa tcagatacag tgcatcggct
     6781 tcttcattcg ggccgttatc cgtgttctgg aaagtaccga ttggcgaaat gcgaatgcca
     6841 atgcgatcgg caccccattc ttcaatcccg gcatcgacca cttccagtac cagacgtgcg
     6901 cgattttcca cgctgccgcc gtactgatcg gtacgatggt ttgaagaagg agaaaggaac
     6961 tgatgcagca aataaccgtg agcagagtgg agctctacca gatcaaaacc ggcttcacgc
     7021 gcgttagcaa tggcctgacg gaaatcattg acgatacctg gaatctcttc cagttcaagc
     7081 gcacgcggca tggatgtttc aacacggatc gcctgaccat tttcatcgcg cagagaagta
     7141 cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct gaccgccagg ttgcaggctg
     7201 gcgtgagaaa tgcgtccggt gtgccacagc tgcacggcca tatgaccatt ttcagcatga
     7261 acgccagcgg tgattttttt ccatgcggca atttgctccg gactatggat gccaggcgca
     7321 cctgcatatc cttttgcctg ggcagaaatt tgcgtggctt cactaataat caaaccggca
     7381 ctggcacgtt ggcgatagta ttccgccatc aacggggtag gaatgtcacc cggttcaata
     7441 ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg ccgccgtgat cgcgcccact
     7501 ttcagtgggg aatacagttt ttcagatgac ataaaggctc ccggtcgtct ggtgataaaa
     7561 aatgcctgct aaacggcagg cgtcgcaata atgtttttta catgggccag cgcgttttcc
     7621 agtggctcga aactgcgcga aattttggcc tgcagattcg cacccagcca tagtgcgtaa
     7681 agcacttgtg cctgttgtag cggttcgcca caaaaggtta aacaatggtt ctcacggcca
     7741 ttttccagcg cctgagagag cagggcgatc acgccgcgag cacctttatc catcgcgctg
     7801 cgcatatctt ctgacagttc gcacacttcg gcagagagtt ttactgtcag gcaaccactg
     7861 atggttccat gttggcaaaa ctggttcagt gtttgctggt aataagccag tatgcggtcg
     7921 cggtagttac cttcgccgga ttgcagcaac tcagtcagtc gctggtgata tgcggcgtaa
     7981 tgacgctcaa gcatggcaac gccaaacgct tctttagagc gaaagtagtg atagaaggac
     8041 cctttcggca cttcagcggt ttttagtaat tcgcttagcc ccatcccggt gaatccacgt
     8101 tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc gagtatcatg ttcggtgtgt
     8161 ttgttcatgc gttggagtgt agtagaccag tcggtctagt gcaagcaaag ttgctacttt
     8221 ggacattaac agcttcaata atggtaataa tcttatctga ggagggcgaa gtggcggagc
     8281 aattagagtt ctttcccgtc cagagcccgt gccggggaat ttgccagtct gacgaacgcg
     8341 ggttttgtcg cggctgtttt cgtagtcgtg atgagcgttt taactggaat aaaatgagcg
     8401 atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg attaatgcgc aaattacgcg
     8461 caaataagcc agcgtcatca gacgaaccgg agcaaccatc actcttttga acctaatctt
     8521 gcgtatactc ctctcatatc tgataagagg aagcgattat ggttcagcgt attactattg
     8581 cgccgcaagg cccggagttt tcccgttttg tgatgggcta ctggcgattg atggactgga
     8641 atatgtccgc ccgccagctg gtcagtttta ttgaagagca tctggatctc ggcgtgacca
     8701 ccgtggacca tgctgatatt tatggtggct atcagtgcga agcggcgttt ggcgaggcac
     8761 tgaaactggc acctcacctg cgtgaacgga tggaaatcgt cagtaaatgc ggtatcgcga
     8821 cgaccgcgcg tgaagaaaac gtcattggtc attacatcac tgaccgcgat cacatcatta
     8881 agagcgccga acagtcgcta attaatctcg cgaccgatca tctggatctg ctgttaatcc
     8941 accgaccaga cccgttaatg gatgccgatg aagtggcgga cgcgttcaaa catctgcatc
     9001 agagcggcaa agtgcgtcat tttggcgtat cgaactttac gcctgcgcaa tttgccctgt
     9061 tgcaatcacg tctgccgttt acccttgcca ctaatcaggt ggaaatatcc ccggtgcatc
     9121 agccgttact gctggatggc acgctcgacc aactacaaca actgcgtgtt cgtccgatgg
     9181 cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga ttatttccag ccgctgcgtg
     9241 atgaactggc tgtggtggct gaggagttaa acgcgggctc gattgaacag gtggtttacg
     9301 cctgggtatt acgtttacca tcgcagccgc tgccaattat cggttcaggt aaaattgagc
     9361 gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac ccgtcaacaa tggtttcgta
     9421 tccgtaaagc ggcactgggg tacgacgtac cgtaagcact tttaggaata gccgccgttc
     9481 aaaaatgtgt cactggttta cacttattca ggaatgcaca atgaacggag gtcctatgaa
     9541 acgttttagt ctggctattc tggcgctggt tgttgcaacc ggcgcacaag ctgccagtga
     9601 aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg cagtcaattg gtagcgtcac
     9661 cattactgaa accgataaag gtctggagtt ttcgcccgat ctgaaagcat taccccccgg
     9721 tgaacatggc ttccatattc atgccaaagg aagctgccag ccagccacca aagatggcaa
     9781 agccagcgcc gcggaatccg caggcgggca tcttgatcca caaaataccg gtaaacatga
     9841 agggccagaa ggtgccgggc atttaggcga tctgcctgca ctggtcgtca ataatgacgg
     9901 caaagctacc gatgccgtca tcgcgcctcg tctgaaatca ctggatgaaa tcaaagacaa
     9961 agcgctgatg gtccacgttg gcggcgataa tatgtccgat caacctaaac cgctgggcgg
    10021 tggcggtgaa cgctatgcct gtggtgtaat taagtaatta cgaggccagc gtaccttgcg
    10081 gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg ccagacaatt gccgccagct
    10141 cacgggcggc aggttgatga tgacgggcaa ggctgttgca aatccgctgt aattcttcaa
    10201 gtgtggccgc cagtgatttt tgctgaacgc cacgctcact catcactccc cgcaacagtg
    10261 aaatacagtt atcccgtact cgcgataacg gatcggaacg cgattcccag tcgcgcaatt
    10321 gccagacaac atgagaacag ttcagcagca ctacacccca gcgtaataac caacggcgag
    10381 ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg ataagtgagc gattcaaatt
    10441 cgctttcact cagtgttgga tggcggctta gctgatcgac aaaatcccgg cgcagcgcgc
    10501 gaatatggcg gcggctttta cgagcatccg atcctggacg cagaatggcg aacgctagcc
    10561 aagccagcgc aacgccaacg atttttgcca gattatcgtt aagaaaatca gcaaaatcat
    10621 acaccggggg attagtgaca gcgataaacg aacccataaa aacaatcagt tgcccccaca
    10681 gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat tgtcgccagc agtggaaaga
    10741 gaaacagtaa aaattgccac agatcgctaa tctggaccat caggccaaat ttgaccacaa
    10801 agctgaatag cgaaagtaac accagcgtgc gcatcagcag tgacaacgac ttaaacggtg
    10861 ctgcgacggc ggagtagagt acgcagctaa ttgctgccag cgttaatgca ttggcaccgg
    10921 catcccattg cgatgcaata ctccatgcgc caatcatcat caacgtacaa aatgtacgca
    10981 gcccgctcca catagcttcg gcgttgtcgg tatgacgagc cagagcggat gtccgtgcga
    11041 gtctggcatg atcatctaca ccgctttgca gacgatgtaa ttcctgacta ctttgcagat
    11101 aaaggcggca aaaataacgt agtcgctgcc agaaggcgac gtgccggtag tcggcgacgt
    11161 tggtcgggcg tagcggggcg ataatacgtg cgacggtgta agcatctgtt tgcgaactgg
    11221 cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc acctggcggt gagggccagt
    11281 tgagcaacat acgtcgcagg ctggagatga cactggtcat acgtaattgc tggtggagta
    11341 gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg gctccagaaa gcctggatac
    11401 gcagcaaatt catggtcagt atctgcccaa tcaccccctc atgtgctgca cgaatggcat
    11461 cgtttgtttc aggctgccag agtaaactgg catgttccag taatcgggcg tgcatgtttt
    11521 tcaatgcggt taaaagggct gtagcatcgg aactgctagg caggatcatc atcatcatgc
    11581 cgccgcacaa aataccgact atcacctcgc aaacgcgcgc ctgagcgata tcccacagct
    11641 ggctggcctc agtaatatta accatcggaa aggcgatgat ggcagccgtg tagcccgcca
    11701 gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca ggcccaggta caaaagccaa
    11761 gccacgccga catgctcaat agaaaaaacc acggctcatt gagcgtatgc ccggcaagaa
    11821 gcagtgccgc aatggctccc agtaaactgc cagcgatgcg tccgaggctt ttgctgatga
    11881 caccgccaac ggtgggaaag ctaaccactg cagccgaggt catcgcccaa tagggttcat
    11941 ccagattcaa ataataggca accgtcagcg ccagacacat ggcaatggta ttgcgtaacg
    12001 cataacgcca ttgcgccagc gtggccttga accagggcaa attgcgcaag gaccatgacg
    12061 atgcgttcat tattgaccta cggcaatgct acaggtggtg cccgacacca gcgtgacatc
    12121 gcctggaact ttatccagcg caaagcggac gggaacgcgt tgggcgagac gaacccaggg
    12181 gacgttaggt ttcacatccg gaataaggct tgagtcactt tcaacgcttt gatcataaat
    12241 cgcccgaccg atactggaaa cgtgaccccg taacgttttg ttatcactat agagggtaat
    12301 ttgtgctggt gcaccttcgc ggatatggcg caacttggtt tcttcaaaat aaccaatgac
    12361 gtaaaacgag tggctgtcga caagggcaaa cagcggtttc ccggtgtcag cgtaatcgcc
    12421 gatgcgggta gtgaggttag tcacccaacc actcaccggg gcgcgaattt ccgtttgcgc
    12481 cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca ttaacactgg cctgcatcgc
    12541 tttaacattg aggttagcgg tatcgagttc ttcggcagaa ataaaatttt gtgagagatg
    12601 acgacgacga ttcgcttcgt tattggcttt tgcgaggtca gattgcgctt tcgccagctg
    12661 agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta tcgatcgtga gcagaagatc
    12721 ccccgcgtta accagttggt tgtcttttat attcagctca acaatacgtc ccgacacctg
    12781 tggggtgata gaaacctttt ctgcgcgtat tttcccgtcg cgtgtccacg gagattgcat
    12841 gtaataattc cacagccacc atccggcaag aaccgcaact atcgctacaa tgattgttga
    12901 gaaatactta attgttttaa ttgacatagt taccacgcaa tcagtattgc cagagcaagg
    12961 caaacgcaaa tcgcaaacag cgataaatcc attaacaagg gatgccagat gtcaccggcg
    13021 tagatccagc cacgaagcag gcggtgtacg acaagccaga tgacgaatcc aaacgcgaag
    13081 gctttgaaaa acggaggaaa atagacggac gcaccgaaca ccagatcttg taagggcaat
    13141 cctgttgcat tgagcataaa cttcacaaag tagattcctt tacgaccgaa tatattgcgt
    13201 tagattaata aatattcttt aagtgcgaaa aatttacgcg caatttctga aaaatatgtc
    13261 tgacggtaac caaatgcagc aatacatttg ttttagcaat acaattgctg cacactattc
    13321 taaaagccgc ataatatctt agcaagctaa ttataaggag atgaaattgg aatcgccact
    13381 aggttctgat ctggcacggt tggtgcgcat atggcgtgct ctgatagacc atcgcctgaa
    13441 accgctggag ttaacacaaa cccattgggt tacgttacac aatatccatc agttacctcc
    13501 agaccagtcg caaattcaac tggcaaaagc gattggcatc gagcagccat cactggtccg
    13561 tactctggac caactggaag aaaaagggtt aatttcgcgt caaacttgtg ccagcgatcg
    13621 tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca ctgatcagcg aaatggaagc
    13681 tgttattaac aaaacccgcg cggaaatatt acatggcatc tccgcagagg aactggagca
    13741 actgattacg ctcatcgcaa aacttgagca taatatcatt gagttacagg ccaaagggtg
    13801 aaatgaaggg ggcgtgtggt cagtaacctg accacacgca aacttattta gcgcggagaa
    13861 acggtcacct gactgccatt gctggccagt acgacacgtt ggcccggaga gaaacgagtg
    13921 ttgccttgtt tctgtaccac catgatggta ttaccatcgt ctttacgaat ttccagctcg
    13981 acaccctgcg ttttgttcat tgcactctgt acgccctgac cagctacgcc acctgcaaca
    14041 gcgcctgctg cagtagccag agaacgcccg gttccgccac caacagtatt ccccaggaaa
    14101 ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg aatcatcacc gccctgaatc
    14161 tgtaccggac gtacgttaac gatggtgcca tagctgacat tctgtacttg tttcgcttca
    14221 gaagcggtat aaacatcccc tgacagggtg tcgttattaa cacaaccgac aagagacaga
    14281 cctaccattg aaacaaccaa tacgcgttta atcattgaaa aatctcctgt tcaccatgaa
    14341 acgccacgca agcatccctc atggtcaaag tatatggcat atgtgaatcc atggttacat
    14401 gttctaccaa atatgagcaa atcataatga aagtaggatc aaccaattat aaacaaaaca
    14461 ttcatgatga ttattgattt gaagataatg ttttactacc aacggtaaaa atcggtcatt
    14521 ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg caaacgtcag attgaaaaag
    14581 gatgaactat gaaatcgggc cgctttattg gcgttatgtc aggcaccagc cttgatggtg
    14641 ttgatgttgt gttggcgaca atagatgaac accgggtcgc acagctggca agcttgagct
    14701 ggccgatccc ggtgtctctg aaacaggctg tactggatat ttgccagggc cagcagctta
    14761 cactttcgca gtttggacag cttgatactc aactcgggcg actttttgct gatgcggtca
    14821 atgccttgct taaggaacaa aacctgcagg cgagagatat agttgcgatc ggctgtcacg
    14881 gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca cactttacag attggcgata
    14941 acaatcaaat tgtggcacgc accggaatta cggttgtcgg tgattttcgc cgtcgcgata
    15001 ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt ccatcatgcc ttgctggctc
    15061 actcaaccga gcgacgaatg gtgctcaata ttggcggcat cgccaatctg tcactgctca
    15121 ttcctgggca gccggttggc ggctacgata ccggtcctgg taacatgctg atggatgcct
    15181 ggatctggcg tcaggccggt aaaccttacg ataaagatgc cgagtgggca cgggcgggta
    15241 aagttattct cccactgctg caaaatatgc tcagcgaccc atatttctcg caacctgcac
    15301 cgaaaagcac cggacgcgaa tactttaact acggctggct ggagcgccat ttacgccatt
    15361 ttccgggtgt tgatccccgt gatgttcagg ccacactggc agaactcacc gccgtgacca
    15421 tttctgaaca agttttgttg agcggtggct gcgaacgatt gatggtatgt ggtggaggta
    15481 gtcgtaatcc gctactcatg gcgcgtctgg cggcattact gccaggcaca gaagtcacca
    15541 ccaccgatgc cgttggcatt agtggcgatg acatggaagc attggctttc gcctggcttg
    15601 cctggcggac gctggcggga ttaccaggaa atctgccttc cgtcactggc gcaagccagg
    15661 agacggtact gggggctatt ttccctgcta acccgtgata tcagagttaa ctgaattttt
    15721 cttaccgcgt ctgccgttac acttggaacg ttagggaggg cgtattgccc tccagaccag
    15781 gaaagtcttc gggaaatgtc tatgaaaaaa ctgttactga tctgtttgcc ggtgcttctc
    15841 accggctgta gcgcgttcaa tcaactggtt gagcacatgc aaaccgctac gctggaatac
    15901 cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc cgcgccagga agtcagtttt
    15961 gtttacgata atcaactact gcatctcaaa cagggcattt cagcgtctgg tgcacgttac
    16021 actgacggaa tctatgtttt ctggtcgaaa ggcgatgaag cgactgtcta taaacgcgac
    16081 cgcatcgtat tgaataactg tcagttacaa aatccacagc gttgagattt ttccaggggc
    16141 ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa accatgtctg ataacgacga
    16201 attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa ggcgggttac gccgtcgcga
    16261 tcttcccgcc gatccattaa ccctttttga acgttggctc tctcaggcgt gtgaagccaa
    16321 actggcagac cctaccgcga tggtggtcgc taccgtggat gaacatgctc agccttatca
    16381 acgcatcgtt ttactcaaac attacgacga aaaaggcatg gtgttttaca ccaacctcgg
    16441 tagccgtaaa gcacatcaac ttgaaaataa tccgcgcgtt agcctgctgt tcccgtggca
    16501 tacccttgaa cgccaggtga tggtgatcgg taaagcagaa cgactttcga ctctcgaagt
    16561 gatgaaatat tttcatagcc gcccgcgtga tagccagatt ggtgcatggg tttcgaagca
    16621 gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa ttcctggagc tgaagcagaa
    16681 gtttcaacag ggcgaagtgc cattgccgag cttttggggc ggttttcgcg tcagccttga
    16741 acagattgag ttctggcagg gcggtgagca tcgcctgcat gaccgctttt tgtaccagcg
    16801 agaaaatgat gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa aaatcttgct
    16861 ttaatcgctg gtactcctga ttctggcact ttattctatg tctctttcgc atctggcgaa
    16921 aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg agattttgat ggcaagcagt
    16981 aacttgatta aacaattgca agagcggggg ctggtagccc aggtgacgga cgaggaagcg
    17041 ttagcagagc gactggcgca aggcccgatc gcgctctatt gcggcttcga tcctaccgct
    17101 gacagcttgc atttggggca tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg
    17161 ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc tgattggcga cccgagcttc
    17221 aaagctgccg agcgtaagct gaacaccgaa gaaactgttc aggagtgggt ggacaaaatc
    17281 cgtaagcagg ttgccccgtt cctcgatttc gactgtggag aaaactctgc tatcgcggcg
    17341 aacaactatg actggttcgg caatatgaat gtgctgacct tcctgcgcga tattggcaaa
    17401 cacttctccg ttaaccagat gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa
    17461 gatcagggga tttcgttcac tgagttttcc tacaacctgt tgcagggtta tgacttcgcc
    17521 tgtctgaaca aacagtacgg tgtggtgctg caaattggtg gttctgacca gtggggtaac
    17581 atcacttctg gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt tggcctgacc
    17641 gttccgctga tcactaaagc agatggcacc aaatttggta aaactgaagg cggcgcagtc
    17701 tggttggatc cgaagaaaac cagcccgtac aaattctacc agttctggat caacactgcg
    17761 gatgccgacg tttaccgctt cctgaagttc ttcaccttta tgagcattga agagatcaac
    17821 gccctggaag aagaagataa aaacagcggt aaagcaccgc gcgcccagta tgtactggcg
    17881 gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac aggcggcaaa acgtattacc
    17941 gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag cggacttcga acagctggcg
    18001 caggacggcg taccgatggt tgagatggaa aagggcgcag acctgatgca ggcgctggtc
    18061 gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa ctatcgcctc caatgccatc
    18121 accattaacg gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga agatcgtctg
    18181 tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt actgtctgat ttgctggaaa
    18241 taatgcatta agtggaaagg gggagtgaga aatcactccc cctggttttt atacagggaa
    18301 catgatgaaa aatattctcg ctatccagtc tcacgttgtt tatggtcatg cgggtaacag
    18361 tgcggcagag tttcctatgc gccgcctggg cgcgaacgtc tggccgctga acaccgttca
    18421 attttctaat cacacccaat acggcaaatg gactggctgc gtgatgccgc ccagccattt
    18481 aaccgaaatt gtgcaaggca ttgccgccat tgataaatta cacacctgtg atgccgtgtt
    18541 aagtggctat ctgggatcgg cggagcaggg tgaacatatc ctcggtatcg tccgtcaggt
    18601 gaaagccgcg aatccgcagg cgaaatattt ttgcgatccg gtaatgggtc atccggaaaa
    18661 aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg cggcacggtt tgcctgccag
    18721 cgatatcatt gcgccgaatc tggttgagct ggaaatactc tgtgagcatg cggtaaataa
    18781 cgtcgaagaa gcggttctgg cagcgcgcga actcattgcg caagggccac aaattgtgtt
    18841 ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt tttgaaatgc tgctggtcac
    18901 cgccgatgaa gcctggcata tcagccgtcc gctggtggat tttggtatgc gccagccggt
    18961 aggtgttggt gatgtgacga gcggtttact gctggtgaaa ctgcttcagg gggcaacgct
    19021 gcaggaggcg ctggaacatg tgaccgctgc agtctacgaa atcatggtga ccaccaaagc
    19081 aatgcaggaa tatgagctgc aagtggtggc tgctcaggat cgtattgcca aaccagaaca
    19141 ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg cccacaacga cctgtgggcc
    19201 gatggatatt actttaagcc ttccgctgac agcgcgtctt gtacttccgg acgttcagcc
    19261 atacgttgca taaatgctgc aatgtgctcg aacccttcca gattcagttt caccgcgtat
    19321 gcccagcgca gaaccgtaaa cagataggca tcagcaattg taaatctttg cccgcagatc
    19381 cagtgctcat ccttcagtgc ctcgttcaca tattgcagct tcttctccag ctgcgcgcga
    19441 actgtcggtt tgtactcttc cggtgtatca gggcgaaaca gaggtgtgaa acctttatgc
    19501 agctcggtgg cgatgtaatt cagccattcg atggttttat agcgggaaat actgtttacc
    19561 ggtgccagca actggcggtc ggggacgctg tcggcaagat actgcataat cgctacgcct
    19621 tccgtcagca aagtaccgtc atccagcagc aatgcaggca cctgcccctt agggttaacg
    19681 acaaagtaat cgtcaccgtt ttcgagacgt tttttcatta aatccacact gacgagggta
    19741 aaatcctttc cgctctcatg cagggtgata tgggaagcga gagagcaggc acccggtttg
    19801 tagaacaatt tcatcggtaa ctccttttgt ctgtggttta aagtatggta gtgcgttgct
    19861 gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt ccctgaattt
    19921 tacgctacag cagctttcgc cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt
    19981 ttaggcgcgg tcagcagcat cagtactgca ataatggcag tagcgacacc aatctgcaag
    20041 aatacgcgac cataaacttc cagtgacatc agcgggtcgg taacgttatc cggcacagcc
    20101 atcatacccg caacataacc accaatcagg tttgcaccgg cagtggtcag gaaccagcta
    20161 cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc cagacccaga
    20221 ccagagatca tcagttcccc gatgctctgc aggccatagc ttgcgaccag ccagcttaca
    20281 gacacgatac cagcgtcaga cgcgaatttc gcacccagcg gcagaatcag gaacgcacca
    20341 gaacacatca ccatgccgat tgcaaacttg gttggcatcg gcagggtatc gcccatcttg
    20401 ttatagatag cggccagaat cggactaccg atgatgatcc agaacgggtt cagtgcctga
    20461 tactgctcag gttctacggc cagacccaga atggagtgct caacgttacg aatcgcaaag
    20521 aagttcagtg acgttggcat ctggctgtac agcacgaaga agataatggc ttcgagcatc
    20581 aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta
    20641 ccgaagataa ccacgatacc gaaggcaaca acgcccagcg ccatacgcgc aacttcctga
    20701 ttgtgcagca gccaggtggc gatagcgatc agtgccacaa caccaataat ggtcagcagc
    20761 aggttacggt agttgatagg ctcgaagtct ggttttgaac cgtactgttt aacccagcgt
    20821 tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa cgcaaacgca
    20881 acactccagc cgtatttcgc ggccagccac ggcgtagcaa tcatagagaa gaaagagccg
    20941 atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgcgggtc gtttttctca
    21001 tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc gaccgcaata
    21061 gccgccatac ccatataaac gataccggcg tcgtgaccag accaggcaac cagagcataa
    21121 ccaatcgcca gcacaatagc gccgagcata attacgcgtt tagtacccag taccttgtca
    21181 cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga agagaaaagg
    21241 gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc cataattcct
    21301 tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag atagaacgcc
    21361 ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg gtttttggtt tgcagtggac
    21421 acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg ttatgtctaa
    21481 aaaacatacg cgcaattgtt atagtaggag gggaaacggc aggtaatgtt cactatcttg
    21541 cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg ggaattcaac
    21601 cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc agattttttc
    21661 actgcactga gaggggggtg atggcttaac ggcgatatgt tctgctattt agcgttttta
    21721 ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgtag gtcaatattt
    21781 gttgcgtgtg taaatttaaa tgattttaac ctgattcttg caatgttttt acattgcgac
    21841 agcatcgtga tcttgatcac gaatgaatag aaaatttcgc tattaaaaaa acaattaacc
    21901 tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa tggcatcatt
    21961 cacgttaaaa agaagtttta aaagaggata aagaaaggtt atcaatgggg taatcggtgt
    22021 tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt caataataca
    22081 agagccacag cggggcttgc gggcaatgca ggtataacgc ccgtgcagga tcaaccaatg
    22141 gtggcagtcg actttaaact ctgccggaac cactttcagt agcttttctt ctacctgttc
    22201 gacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt gcgtgtcgac
    22261 agcaatagtc ggccagccga atgcagtgtt taatacgacg ttggctgttt tacgacctac
    22321 gccgggcagg gcttcaagcg cagcacgatc ttccggaacc tcgccattat gctgctccag
    22381 caagatacgg ctggttttga tgatattttc tgctttgctg ttataaagcc caatcgtttt
    22441 gatataggtt ttcacccctt caacgcccag ttcaagcatc gctgcaggcg tattcgccac
    22501 cgggtagagt ttcgccgtcg ccttattaac actgacatcg gtcgcctgag cggaaagcag
    22561 tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcggtggtgg gatgaggatt
    22621 gttctcacgc aggcgagtga ggatctccag gcgttttgct ttgttcatca gacattccct
    22681 gtttcaccgt ttggcaatgc acgttcggca gctgcttcag tacggcgctt tttcattctt
    22741 tcatcaatca ggtattttcc tgccagcatc agtcccaggc caataaatgc acctggaggc
    22801 agcatcgcca gcaggaaagg ggagtcggtg tggaaaattt ccacgcgtaa tacttttgcc
    22861 cagctaccta acagcgcatc tgcaccgtca aacaatgtgc cattgccgat aatttcgcgt
    22921 agtgaaccca gcacgaacat ggcgcaggtt gcgcccatac caattgaaaa gccgtccagt
    22981 gccgaaagcg ccggaccttt tttggcggcg aaggcttcag cgcggcccac aacgatacag
    23041 ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa ggcgtaggcg
    23101 ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta aatgggaatg
    23161 cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg tcaggttggt cagcgtcagt
    23221 accagcgtag tcgcaagtcc taaacccaga gcgttagtgg cagtggacgt gaccgccaac
    23281 agaggacaaa ggccgagcaa ctggaccagc gcagagttgt ttttccacaa cccctgaaca
    23341 ataacgtctt taatttcgct cacgctttat tctccacagg caggaagttg agaaagttgt
    23401 gccggtaacg tctgagcgta caatccggcg cgttttaccg cattaaccac cgcgcgggga
    23461 gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat ctttcttcac cgcccagtgc
    23521 gcatcatctg caccactgat ttttttaccc gcaaaatggg tgatccagtc agaaaggcgc
    23581 agttcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg cgtgccaagt
    23641 accgtgccgt taaaatcggc tcccaccagc agctgaatcg caccggaata gccatctggc
    23701 gcggttgctt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat gtaaacccga
    23761 tgctcacctt tacctaactc tggcgcagtt accagatagc aactctgtgc cagcgcattg
    23821 ttatagcgtt cggctggcag cacctgatca aataatgcct tttgttgcag actggcctgt
    23881 tcagcaatcg tcgttttggt catctggttg atggccgcag ttaaccctgt tgaacccgct
    23941 gcaaatagcg ctaacgtaat gccgtgtttt cggatagttt tcagcatggt ttaccctttg
    24001 cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt gatgttcgcc
    24061 agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat caaccagact
    24121 aataagcccg caagcgcgcc gaaaataaga cgaccacgat tggtcgtaga agcggtaacc
    24181 gggtcagtca aaataaagaa tgcgccgagc atggtcgcac cagacagcag atgaatttgc
    24241 ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa cgccagcgtt
    24301 actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa ccacaggccg
    24361 ccagccagcc aggcgagatt tacccattgc caaccagcgc ccgccagaat accgctgtag
    24421 atcggatatt gcataatctg ttcaaccgaa tgaccggcac ggacagaggt tttaaatgta
    24481 tccagcggtg tcgcctgact aatgccatca atacctaagc gtagtgtgtt catatcacca
    24541 ccactggcgg tatgcccgct gaaaataacc tgtatggcgt cgataaaacc agggatgttg
    24601 accgcaattt catgtggcgg taaccagctg gtcatctgca ccgggaagga gatcagtaag
    24661 accacataac caatcattgc tggattaaac gggttttgcc ccagaccgcc atacaactgt
    24721 ttagcgatga tcaccgcaaa caccgtaccc agcacgacca tccaccatgg cgcgaggggg
    24781 ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt caacgttgcg
    24841 gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagc actaaccgat
    24901 gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc tgcgattcct
    24961 ggcacggctg cgagcaacac caacagcata atgcgcgatg tctggcgctg gttatgggta
    25021 taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt ctgctgggcg
    25081 gcttttttgg cctgagcgcg ggcaatagcc gcggcaactg ccgctttgcg cggatcaacc
    25141 tgttcttctg gttctgcgtt agtctgttgc tgttccagct tgcgcgcttt ggcacgggca
    25201 atagcggctt cgacggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc
    25261 tgttgctgtt ccagtttgcg cgc
//
LOCUS       DAHBHC010000075        25058 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHBHC010000075 DAHBHC010000000
VERSION     DAHBHC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.075"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(27..1097)
                     /gene="paaK"
                     /locus_tag="M2A35_003681"
     CDS             complement(27..1097)
                     /gene="paaK"
                     /locus_tag="M2A35_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HCB7199927.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(1105..1602)
                     /gene="paaD"
                     /locus_tag="M2A35_003682"
     CDS             complement(1105..1602)
                     /gene="paaD"
                     /locus_tag="M2A35_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HCB7199928.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(1617..2363)
                     /gene="paaC"
                     /locus_tag="M2A35_003683"
     CDS             complement(1617..2363)
                     /gene="paaC"
                     /locus_tag="M2A35_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HCB7199929.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(2372..2659)
                     /gene="paaB"
                     /locus_tag="M2A35_003684"
     CDS             complement(2372..2659)
                     /gene="paaB"
                     /locus_tag="M2A35_003684"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HCB7199930.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(2671..3600)
                     /gene="paaA"
                     /locus_tag="M2A35_003685"
     CDS             complement(2671..3600)
                     /gene="paaA"
                     /locus_tag="M2A35_003685"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HCB7199931.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            3885..5930
                     /gene="paaZ"
                     /locus_tag="M2A35_003686"
     CDS             3885..5930
                     /gene="paaZ"
                     /locus_tag="M2A35_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HCB7199932.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQTLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            6178..8451
                     /gene="tynA"
                     /locus_tag="M2A35_003687"
     CDS             6178..8451
                     /gene="tynA"
                     /locus_tag="M2A35_003687"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HCB7199933.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPTKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8509..10008)
                     /gene="feaB"
                     /locus_tag="M2A35_003688"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(8509..10008)
                     /gene="feaB"
                     /locus_tag="M2A35_003688"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HCB7199934.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            10244..11149
                     /gene="feaR"
                     /locus_tag="M2A35_003689"
     CDS             10244..11149
                     /gene="feaR"
                     /locus_tag="M2A35_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCB7199935.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(11321..11650)
                     /locus_tag="M2A35_003690"
     CDS             complement(11321..11650)
                     /locus_tag="M2A35_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCB7199936.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(11655..11840)
                     /locus_tag="M2A35_003691"
     CDS             complement(11655..11840)
                     /locus_tag="M2A35_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCB7199937.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(11837..14476)
                     /locus_tag="M2A35_003692"
     CDS             complement(11837..14476)
                     /locus_tag="M2A35_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCB7199938.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            14684..15673
                     /gene="ldhA"
                     /locus_tag="M2A35_003693"
     CDS             14684..15673
                     /gene="ldhA"
                     /locus_tag="M2A35_003693"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCB7199939.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            15784..16206
                     /gene="hslJ"
                     /locus_tag="M2A35_003694"
     CDS             15784..16206
                     /gene="hslJ"
                     /locus_tag="M2A35_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCB7199940.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(16203..16469)
                     /locus_tag="M2A35_003695"
     CDS             complement(16203..16469)
                     /locus_tag="M2A35_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCB7199941.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            16743..20267
                     /gene="nifJ"
                     /locus_tag="M2A35_003696"
     CDS             16743..20267
                     /gene="nifJ"
                     /locus_tag="M2A35_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCB7199942.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            20633..21766
                     /gene="ompN"
                     /locus_tag="M2A35_003697"
     CDS             20633..21766
                     /gene="ompN"
                     /locus_tag="M2A35_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCB7199943.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            21907..22341
                     /gene="uspF"
                     /locus_tag="M2A35_003698"
     CDS             21907..22341
                     /gene="uspF"
                     /locus_tag="M2A35_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCB7199944.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(22922..23563)
                     /locus_tag="M2A35_003699"
     CDS             complement(22922..23563)
                     /locus_tag="M2A35_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HCB7199945.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGGLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            complement(23645..24274)
                     /locus_tag="M2A35_003700"
     CDS             complement(23645..24274)
                     /locus_tag="M2A35_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HCB7199946.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNSYFQSVMA
                     EHRENKTSNKILMEKINSCLFRPDSNHFSCPESFLTCPITLDTPETGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDAKREAFCCK"
     gene            complement(24347..24928)
                     /locus_tag="M2A35_003701"
     CDS             complement(24347..24928)
                     /locus_tag="M2A35_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HCB7199947.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHFPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAASFSRLVGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
BASE COUNT         6388 a         6412 c         5935 g         6323 t
ORIGIN      
        1 ttgctgacgg ctgacgatca gttcgctcat gccatcccct tcgcgtcaaa gtcaaccacc
       61 acatcgctgg tcagcggcag tgcctggcaa ctcaacacat agcctgcggc cagttcatcc
      121 ggttccagac tgtaattggt ttccatcgcc actttgccac gcagcacttt gcatttgcag
      181 gtcgcacaga cgccgccttt gcaggcatag ggcagatcag ccccctggcg caatgccgca
      241 tcgagaatgc tttcatcgtc ggcattaagc acgatttccc gatcccgccc atcctgacgt
      301 acagtcactt tttgtccgtc actttgcacg ttaacgctac gtttgacgcg cgtgccaggc
      361 gtattaaacc gctccagatg aatggtttta tctggcattc ccagtgcttt taaggcggct
      421 tccgtctcat ccatcatcgc cgccggacca caaataaatg cctcatcata aagacgaaaa
      481 ttaatgagcg aggccccaag tgactgtaat ttttcaccgt caatacgccc gtgaagcaga
      541 tcgctgtcga gggtttcctg actgaaaatg cacaacaact gtaaacgctg aggatatttg
      601 tctttcaggt ctgccagtgc ctggcgaaac atcatgctct ggctggtacg gttaccgtag
      661 atcagggtga actgactttc aggctcggtt tgtaaagtgg tggcgataat cgccagcatt
      721 ggcgtaatac ctgatcctgc tgcaatagcc agatagcggc cctggcgttc ggcctgtggc
      781 tgatagccga aatgcccctg cggcaccatg acctccagcg tcataccctg gcggatgtgt
      841 tcgcgggcat agcgggagaa acgtccgcct tcaatggctt tcaccgccac actaatttcg
      901 ccaggcagat agctgcggca aatggagtaa caacggcgta attcttcacc atcaaagctg
      961 gcttttaagg tcaaatgttg accggggcga aagcgatacg cctcctgcaa aggctggggc
     1021 accgcaaagg taatggtcac cgcatcacgg gtttccggct ccacttttgc caccgttaag
     1081 gaatgaaacg ttgtcatggc atcctcaaat acatttgaaa taatcgaaag gttcgcggca
     1141 actatcgcag cgatacaatg ctttgcaggc cgtggaacca aattcactga taagtgtggt
     1201 atggacgctg gcgcagcgcg ggcaacgtac ttctggcggc aaatgggcat ggcaactgtg
     1261 tccggcgggc gggctaatgc catactgtcg cagacgctca cgggcatcgg gggtcatcca
     1321 gtcggtggtc catgccgggt cgagttgcag cacaacctga acgggggtaa acccgtgggt
     1381 tgtcattgcc tcacgtatcg cgccaatcaa atgttccgtt gccggacaac cggaataagt
     1441 cggcgtaaag ccgatcaccc atccttctcc catctgtgtc acattacgca ccatgcctaa
     1501 atcggtaatg gtcagcaccg ggatctccgg gtccgggatc tggctgagta gcgcccatat
     1561 ctcatgaacc tgtggcggtg caatggtggc cagacgttgc atacccatct cctctgttac
     1621 cattgctgac cgggcaagac acgctggaga tactgcattt ctgccagcat gggtccaagg
     1681 tgttcggtat gcagtccttt tttaccgcca gtgcgatacg cctgctcttg cggtacgttc
     1741 aatgtggctt cgttgatccc ggcaaaaact tcggcttccc acgctgcgcg taaagtgcgt
     1801 ggatcaaccg caataccctc ttcactcagt gcaatatcaa tctcgtcggc atcgaacagt
     1861 tcggcggtaa aacgccacaa cttgttgatg gcctgctgca tcttttgccc tgatacgtca
     1921 gtaccattgc ccagtcgctc cagccagcca cgactaaaac gcaggtgata acgcgcttct
     1981 ttaattgctt tggcagaaat cgccgccagt tgcggatcac ggctctccat cagacgggta
     2041 aagagcgcca catgccatgc atcgatgaaa tactggcgtg caatggtgtc ggcaaaattg
     2101 ccgtttggct gttcaaccaa caataagttg ctgaactggc gctcgtctcg ggtgaaggcc
     2161 agggtatctt catcgccttc tcccgctaat tcagcggcat acgataagaa gttgcgtgcc
     2221 tggcctaata aatcgagtcc aatatttgcc agtgcgagat cgatttccag ttccggtgca
     2281 tgaccgcacc attcacccaa ccgctgggag agcaccaggc agttatcgcc caggcgcaag
     2341 gtgtaagccg ttaactgatt catttccgac ctcacatgtg ctcaatgcca tcagggatgg
     2401 tgtaaaacgt tggatggcga tagaccttgc tttcagccgg atcaaaaaat tcaccgcgtt
     2461 cttccggttg cgaggcaaca atttcactcg ccttcaccac ccaaattgaa catccttcgc
     2521 tacgacgggt gtaagcatca cgggcatttt ccagtgccat ccgctcatcg gcagcatgta
     2581 aactgccaac atggcggtgt gataagccct gtttgccacg cacgaaaact tcgtataacg
     2641 gccagtaaac attactcatt ttgaatctcc ttatgcgacc ttacgggcat gttgtttttg
     2701 tgcatgggcc agcgctgctt cccgtaccca ggtaccttct tcccaggctt tacgtttggc
     2761 gtcaagccgc tcctgattac aaattccgcg accgttaatt acttcgttaa actcctgcca
     2821 gtcgatctca ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat ccgggtcagg
     2881 aacggtcata ccgagcattt caacctgtgg aacggtgttg tccacgaaac gctggcggag
     2941 ttcgtcgttg gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt ttggcgagtt
     3001 atcatcgttt ggcccgaaca tcattaaggc tggccaccag aaacggttaa tggcgtcttg
     3061 caacatctgc ttttgagctt cgctgccctg cgccagtgcc atgcaggctt caaacccctg
     3121 acgctggtga aaactctctt ctttgcagat tttcaccatt gctctggcat acgggccata
     3181 agaagttcgg cataacgcca cctgattgac gatcgctgcg ccatccacca gccagccgat
     3241 aacgccgata tcagcccaac tcaatgtcgg ataattaaag atggaggaat atttcatccg
     3301 tccgtcgagc attttttggt agatgtcttc tctggcacag cccagtgttt cggcggcgct
     3361 atagagatac agaccatgac cggcttcatc ctgcactttg gctaacagaa tggctttacg
     3421 ccgcaaggtt ggtgcgcgag tgatccagtt accttcaggc aacataccaa caatctcgga
     3481 gtgcgcatgc tgcccaatct ggcggatcaa tgtctttcgg taagcatcgg gcatccagtc
     3541 ctgtggctcg atagccgttt cctgggctat ccgttgctca aagcgttctt cttgggtcac
     3601 gtcatcacct ttacgattcc agttttcgtg aattaattaa aaacacgaat cacattgttt
     3661 tatgaaagtt acacaatggt taactaaaat cacaaatttt gtatgcttga actgtgacgg
     3721 atttcgcacc ctctttttat aaatcattat ttatcagtgc attaatgttt tccgcagttg
     3781 ctatacagat cgcatggtta acatttcatt aaaagatccc ttgcttttta tgattcgcga
     3841 ttcaactatt agcaacagaa atgtgaaaca tctggagagt agcgatgcag cagttagcca
     3901 gtttcttatc cggtacctgg cagtctggcc ggggccgtag ccgtttgatt caccacgcta
     3961 ttagcggcga ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg gctgcccgcc
     4021 tgtttgccat tgaaaaaggt gccccagccc ttcgcgctat gacctttatc gaacgtgcgg
     4081 cgatgcttaa agcggtcgct aaacatctgc tgagtgaaaa agagcgtttc tatgctcttt
     4141 ctgcgcaaac aggcgcaacg cgggcagaca gttgggttga tattgaaggt ggcattggga
     4201 cgttatttac ttacgccagc ctcggtagcc gggagctgcc tgacgatacg ctgtggccgg
     4261 aagatgaatt gctcccctta tcgaaagaag gtggatttgc cgcgcgccat gtactgacct
     4321 caaagtcagg cgtggcagtg catattaacg cctttaactt cccctgctgg ggaatgctgg
     4381 aaaagctggc accaacgtgg ctgggcggaa tgccagccat catcaaacca gctaccgcga
     4441 cggcccaact gactcaggcg atggtgaaat caattgtcga tagtggtctt gttcccgaag
     4501 gcgcaattag tctgatctgc ggtagtgcgg gcgacctttt ggatcatctg gacagccagg
     4561 atgtggtgac tttcacgggg tccgcgacga ccggacagat gctacgagtt cagccaaata
     4621 tcgtagccaa atctatcccc ttcaccatgg aagctgattc cctgaattgc tgcgtactgg
     4681 gcgaagatat caccccggat caaccggagt ttgcgctgtt tattcgtgaa gttgtgcgtg
     4741 agatgaccac aaaagccggg caaaaatgta cggcaatccg gcggattatt gtgccgcaga
     4801 cattggttaa tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc gtggtcggtg
     4861 atcctgcaca agaaggcgtg aaaatgggcg cactggtaaa tgctgaacag cgtgctgatg
     4921 tgcaggaaaa agtgaacaca ttgctggctg caggatgcga gattcgcctc ggtggtcagg
     4981 cggatttatc tgcggcgggt gccttcttcc cgccaacctt attgtactgt ccgcagccgg
     5041 atgaaacacc ggcggtacat gcaacagaag cctttggccc tgtcgcaacg ctaatgccag
     5101 cacaaaacca gcaacatgct ctgcaactgg cttgtgcagg cggcggtagc cttgcgggaa
     5161 cgctggtgac ggctgatccg caaattgcgc gtcagtttat tgccgacgcg gcacgtacgc
     5221 atgggcgaat tcagatcctc aatgaagaat cggcaaaaga atccaccggg catggctccc
     5281 cactgccaca actggtacat ggtgggcctg gtcgcgcagg aggcggtgaa gaattaggcg
     5341 gtttacgagc ggtgaaacat tacatgcagc gaaccgctgt tcagggtagt ccgacgatgc
     5401 ttgccgctat cagtaaacag tgggtgcgcg gtgcgaaagt cgaagaagat cgtattcatc
     5461 cgttccgcaa atattttgag gagctacaac caggcgacag cctgttgact ccccgccgca
     5521 caatgacaga ggccgatatt gttaactttg cttgcctcag cggcgatcat ttctatgcac
     5581 atatggataa gattgctgct gccgaatcta ttttcggtga gcgggtggtg catgggtatt
     5641 ttgtgctttc tgcggctgcg ggtctgtttg tcgatgccgg tgttggtccg gtcattgcta
     5701 actacgggct ggaaagcttg cgttttatcg aacccgtaaa gccaggcgat accatccagg
     5761 tgcgtctcac ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa gaaaaaccaa
     5821 caggtgtggt ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg gtggcgctgt
     5881 attcaattct gacgctggtg gccaggcagc acggtgattt tgtcgattaa tcggtgaatg
     5941 aagggcaacg gcgaatagtt gcccttttat ttcactaaat tttgtgacgt tgtcacatta
     6001 tgcatgatgt gtacatctat tttcagggca tccactgtat gaaaagctgg cacacctgcc
     6061 aaacaacctg gcaggtgcag gcaatcccct ttgcatcagt actgataatg tgaacctgac
     6121 taaaccgccc acagagcgcg gttgctaaca agaacacaac atctgacgag gttaataatg
     6181 ggaagcccct ctctgtattc tgcccgtaaa acaaccctgg cgttggcagt cgccttaagt
     6241 ttcgcctggc aagcgccggt atttgcccac ggtggtgaag cgcatatggt gccaatggat
     6301 aaaacgctta aagaatttgg tgccgatgtg cagtgggacg actacgccca gctctttacc
     6361 ctgattaaag atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc aattgttaat
     6421 ggtcagcctc tggcactgca agtaccggta gtgatgaaag acaataaagc ctgggtttct
     6481 gacaccttta ttaacgatgt tttccagtcc gggctggatc aaacctttca ggtagaaaag
     6541 cgccctcacc cacttaatgc gctaactgcg gacgaaatta aacaggccgt tgaaattgtt
     6601 aaagcttccg cggacttcaa acccaatacc cgttttactg agatctccct gctaccgcca
     6661 gataaagaag ctgtctgggc gtttgcgctg gaaaacaaac cggttgacca gccgcgcaaa
     6721 gccgacgtca ttatgctcga cggcaaacat atcatcgaag cggtggtgga tctgcaaaac
     6781 aacaaactgc tctcctggca acccactaaa gacgcccacg gtatggtgtt gctggatgat
     6841 ttcgccagtg tgcagaacat tattaacaac agtgaagaat ttgccgctgc cgtgaagaaa
     6901 cgcggtatta ctgatgcgaa aaaagtgatt accacgccgc tgaccgtagg ttatttcgat
     6961 ggaaaagatg gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta tcttgatgtc
     7021 ggtgatggca actactgggc acatcccatc gaaaacctgg tggcggtcgt tgatttagaa
     7081 cagaaaaaaa tcgttaagat tgaagaaggt ccggtagttc cggtgccaat gaccgcacgc
     7141 ccatttgata gccgtgaccg cgttgctccg gcagttaagc ctatgcaaat cattgagcct
     7201 gaaggtaaaa attacaccat tactggcgat atgattcact ggcggaactg ggattttcac
     7261 ctcagcatga actctcgcgt cgggccgatg atctccaccg tgacttataa cgacaatggc
     7321 accaaacgca aagtcatgta cgaaggttct ctcggcggca tgattgtgcc ttacggtgat
     7381 cctgatattg gctggtactt taaagcgtat ctggactctg gtgactacgg tatgggcacg
     7441 ctaacctcac caattgctcg tggtaaagat gccccgtcta acgcagtgct ccttaatgaa
     7501 accatcgccg actacactgg cgtgccgatg gagatccctc gcgctatcgc ggtatttgaa
     7561 cgttatgccg ggccggagta taagcatcag gaaatgggcc agcccaacgt cagtaccgaa
     7621 cgccgggagt tagtggtgcg ctggatcagt acagtgggta actatgacta catttttgac
     7681 tggatcttcc atgaaaacgg cactattggc attgatgccg gtgctacggg catcgaagcg
     7741 gtgaaaggtg ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga cacgcgctac
     7801 ggcacgctta tcgatcacaa tatcgtgggt actacacacc aacatattta taatttccgc
     7861 ctcgatctgg atgtagatgg cgagaataac agcctggtgg cgatggaccc agtggtaaaa
     7921 ccgaatactg ccggtggccc acgcaccagt accatgcaag ttaatcagta caacatcggc
     7981 aatgaacagg atgccgcgca gaagtttgat ccgggcacga ttcgtctgtt gagtaacccg
     8041 aacaaagaga accgcatggg caatccagtt tcctatcaaa ttattcctta tgcaggtggt
     8101 actcacccgg tagcaaaagg tgcccagttc gcgccggacg agtggatcta tcatcgttta
     8161 agctttatgg acaagcagct ttgggtaacg cgttatcatc ctggcgagcg tttcccggaa
     8221 ggcaaatatc cgaaccgttc tactcatgac accggtcttg gacaatacag taaagataac
     8281 gagtcgctgg acaacaccga cgccgttgtc tggatgacca ccggcaccac acatgtggcc
     8341 cgcgccgaag agtggccgat tatgccgacc gaatgggtac atactctgct taaaccatgg
     8401 aacttctttg acgaaacgcc aacgctaggg gcgctgaaga aagataagtg attgtttcag
     8461 acaaaaaaac ggcaccaggt gccgtttttt acttatgagc gaaccagatt aataccgtac
     8521 acacaccgac ttagtttcac accaaccgtc cagccagtcg gggccaaaat cacggcccgt
     8581 tcctgactgc ttcatcccac caaacggtaa gttagcgtca attaaggtat ggctgtttac
     8641 ccacaccgtc cctgcctgta agcgatcgct atattccaga gcctgggaga gattttgcgt
     8701 ccagacactg gcagttaagc catattccgt gtcgtttgcc agttgtaacg cctcttctcc
     8761 atccgctact cgcaccaggt ttaccaccgg accaaacacc tcttcacgag ttaagcgcaa
     8821 tttagcatcg ggatttacca ccagcgttgg cgcaacataa tacccctctc cggctggtcc
     8881 attcgacccg cgaatcagct ctgcttgctg tgcctgcgca tcgtcgagga atgaacacac
     8941 tttgtcgcag tgcgcacgag aaaccaaagg gttaatctgt gcaacaggtg acatccccgg
     9001 tcccacttgc aacgatttta ccgcctgctc aaatccacta accagcgtgt caaacaacgg
     9061 cgcttcaata taaattcgcg aactggcggc gcatacttgc ccttgattca ggaagcttcc
     9121 ggtcatcaag ccttcaataa cccattgcgg atcagcatct tttaatacaa ttgccgggtt
     9181 tttaccgccc agttccagcg ttacacgcgt taagtgatca gcagcagttc tggcaatacc
     9241 ttttcccgtc gcggttgaac cggtaaaact gattttcgca acatgaggat gtgacgtcag
     9301 ggccgcgccg catacagcac ctgacccggt gaccacatta aaaacgccat cagggatacc
     9361 agcctcgctg gccagttccg ccacgcgcaa catcgtcagt ggcgtggttt ccgaaggctt
     9421 aatcacgatt gaacagcctg ctgccagtgc tggcatcacc ttccacatac caatcatcaa
     9481 cggaaagttc catggcacaa ttcccgccac tacgccaacc ggctctttac gcgtccaggc
     9541 ctgataacgc gccccctggg gtaagggaat cgacaagtcc agcgttttac ccgcgatttt
     9601 ggtcgttaac ccggcggtat aacgcatcca gttcagcgta cagcccactt caaaagcacg
     9661 ggaaatggca attgacttgc cttgctccag ggtttccagt tgcgccagct cctcactgtg
     9721 ctgttccacc agatcggcaa aacgtagcag aatacgttca cgctctgcgg gtaatcgccc
     9781 ggcccagcga cgcgagacaa aggcccgcca ggcagacatg actgcgttat ctacatccgc
     9841 ttcgttggca tcagcagtag acgcaatttc ttgcccggtg gccggatcaa agatcgccaa
     9901 tcgtttttca ctttgtgcgg ggcctggacg accatcaata taaagaccgt gttgacgatc
     9961 gagaaactgt tggacctggc ttaatactgc tacatgcggc tctgtcatca cttttcctta
    10021 ttatttaccc agtgtgattt cagtgtacgc agcgacaaga aaagtcactt ttttcgctgt
    10081 gacattcgct ttgtcagctg tgacaagctc cgcaaatcgt gacaataacc gcttatgtct
    10141 ctatgcctga caagacttat ttcctatagt caggctcatt agcttcgttt attgcaacac
    10201 aaatgcaaca ataaaaatac atttcacaga gcgaaaacgt gccatgaacc ccgcagtgga
    10261 taatgagttt cagcaatggc tttcccaaat caatcaggta tgcggaaatt ttaccggacg
    10321 cctgctgact gagcgttaca cgggtgtact ggaaacgcat tttgccaaag gactaaagct
    10381 gagcaccgtg acaaccaacg gagtgaattt ataccgcacc tggcaggagg taaaaggcag
    10441 cgacgatgcc tggttttaca ccgtttttca acttagtggt caggcaataa tggagcagga
    10501 tgagcgtcag gtgcagattg gcgctggcga tattacgtta ctcgatgcct cacgcccctg
    10561 ttcgctttac tggcaggagt cttctaaaca gatttcatta cttttaccac gcactctgct
    10621 ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa agactggacg ctgacttacc
    10681 catggtgcaa ctcagtcatc gcctgttaca ggagagcatg aataacccgg cactttctga
    10741 aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg ctgcgcccgg tacttcatca
    10801 gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa aaagtggtta cgctgataga
    10861 cgataatatt cgcgaagaga tattacgccc ggagtggata gccggagaga caggtatgtc
    10921 agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg gtggtcgcgc aatatattcg
    10981 taaccgtcgt ctcgattttt gtgctgatgc gattcgccat gccgcagatg atgaaaaact
    11041 ggcaggcatt ggttttcatt ggggattttc cgaccagagt catttttcaa cagtatttaa
    11101 gcaacgcttt gggatgacgc caggcgagta tcggcgtaaa ttccgctaac taattcgcgt
    11161 tgcatgtact gcaacgccaa ttagttaaaa cagatatttg actatgcatt tttcagggca
    11221 aaaggcaatt taacaggagt tttttaatat tcaccttgtt tcaggaaaat agatcaatcg
    11281 ctttctcctg tagcattatc ccgatattgc ggttttgcta ttaaaatttt cgcacccatt
    11341 taccgtttat cccttgtaca tattcccccg gtttggcccg agctacgagc ttttgcccgg
    11401 cgagtttcgc gatatcatct accgacacat tattttgctt tgccagttgt tggtaactcg
    11461 ctttacgttc ggcattaata tcggttacta atttctctgt ttcagcatcc gttttcagcg
    11521 caaccagata accataaaat gtttcaccta cccgcccctg agttctggct tcatccagag
    11581 tcaatgccat tacattgctg cttaccagcc caacaagaaa cgcacaaaga agtaatgttt
    11641 tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc gacatctttg
    11701 tctgccttga tgatgatctc atgctcaatt ttaacgttca tattgatagt gatcggttcc
    11761 ttaggtgcag cgacttcaat gcgaggcgta cagccaacca gcataaaaga tgacgtcaac
    11821 gcagccagta aaattttcat tgtttttcct cacactcttt tccttgcgga cagtcatttc
    11881 ccggcagacg tgcgttctgc tccaaccatg cctggagatt atcgccaaag cgtaaactgc
    11941 gccacagcgt aaaaatattc tcttcatgat ggtaattaag gtttaccgta ccgcttttac
    12001 catcaacgcg actggtacct gtaatgttgg cctgcatggt taataaaccg agattatcta
    12061 agttaatttt tgtcgatgac cggctaattt ccatatagcg caaccagtta attgctgaac
    12121 ccgcagtcat attgtctttc accaccgcat ctgcggtgtc tttatccagc cgcaaggtca
    12181 ttggcccgct atttgccagc caaccatctt tcactatcca tttttcgttg tttagccata
    12241 atggcaatgc accactaaat gccccggaca tggcgaattg tttcggattg acggcgctaa
    12301 ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac
    12361 gtaattgttt catcagtacg ttaccgccta acacatcgac gctaacatca gtcaacagca
    12421 agggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata tttttcgctg
    12481 tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc cccagatgcc
    12541 aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc tgattatctg
    12601 gcatccaggc actaccgcct ttcaacacgc cgtgccctcc cgcgcggaat ccttgttccg
    12661 gcgcagcaga aaatgcaacc tgagcataca gttcaccatc gcgtaagttc atcttccagt
    12721 cgggtggcac cagcggctgg aatacagtca gtgattgttt tggccaccag gcgttaccgc
    12781 gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca ccagcatgta
    12841 aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat tttaaggttg
    12901 atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt gacagcgcgc
    12961 cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg agaattaatc
    13021 gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg gagaggtcgg
    13081 tcagcgtaat cgtgctgtca tgccattcac cttttcctgc gacatcccag gtggcattca
    13141 tcggtgtaaa actgcctttt ccccagtagc gccactgcca gcggcccgcg tcagggagaa
    13201 aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcattt tcatgcgcct
    13261 gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca cccgctaaag
    13321 gccagcggat ttcatcgatg tccagcgaat cgatgactct tccctttgaa cgaagtaacg
    13381 cgcctggctc aaaggtcagc gttgggtcag acagacttcc acttagctgc gcaggtaaac
    13441 gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagctgcaga ggcagctgac
    13501 tgttatccat gcttaatttt cctgggccaa aatttagcac cgcgttgcct ttacccgctt
    13561 gcccctgggt cagcacactc agtcgtccgc tgatcagagc gttctcaagc cctgcctgcc
    13621 agttgtcgac tttgacaccc agtcggccac tcaaaggaaa acctgcatac ggccaactcc
    13681 agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa tcgagcaacg
    13741 gatcgccgtt atcccgtgcc agcacaatca attgcccgct attttcctgc cagtccagct
    13801 ctgcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca tgcccactta
    13861 caggaagtcc atccggcacg agcggcatag taaattcccc caccagttta accggcggct
    13921 gattttcaaa cgcgacgaca tccagttcgc tgactgtaag ttgttgccct ttcagctggc
    13981 cttgaaattt aactttttcg ccctgataac gcagttgctg gatatcagag gttaatgcga
    14041 gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg atattgatcc
    14101 aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct ggcgattgct
    14161 ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccgtgccg acgttcaata
    14221 accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga caatctccca
    14281 ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg ctttcatcta
    14341 atgcaatacg tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc cacagcccga
    14401 gcgtcatcaa cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg agaacggctt
    14461 tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa gcattcaata
    14521 cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta agcatagttg
    14581 ttgatgaatt tttcaatatc gccatagctt tcaattaaat ttgaaatttt gtaaaatatt
    14641 tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac tcgccgttta
    14701 tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct ttggctttga
    14761 gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca atggctgcga
    14821 agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag agctgaaaaa
    14881 gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg accttgacgc
    14941 ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag aggccgttgc
    15001 tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg
    15061 tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt atggcaaaac
    15121 ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt
    15181 tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg aactcggtgt
    15241 ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc tgcactgccc
    15301 gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg
    15361 cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcag caattgaagc
    15421 gctgaaaaat cagaaaattg gttcgttggg tatggacgtg tatgagaacg aacgcgatct
    15481 gttctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc gcctgtctgc
    15541 ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag ctctgaccag
    15601 tatttctcag actacgctgc aaaacttaag caatctggaa aaaggtgaaa cctgcccgaa
    15661 cgaactggtt taatcttgcc gctcccctgc aatccaaggg agctgattca gataatcccc
    15721 aatgaccttt cattctctat tcttaaaata gtcctgagtc agaaactgta attgagaacc
    15781 acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc tgatggcggg atgtgtaagt
    15841 aatgacaaaa ttgctgttac gccagaacag ctacagcatc atcgctttgt gctggaaagc
    15901 gtaaacggta agcccgtgac cagcgataaa aatccgccag aaatcagctt tggtgaaaaa
    15961 atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact gtctaatggt
    16021 gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc gcagcttaat
    16081 gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg
    16141 aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg
    16201 aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg
    16261 ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg
    16321 ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa acctggcgct
    16381 acgtgcgccg cagtcgcctg ctgaacaggt tcactactac acgccgacaa caataaagcg
    16441 gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga
    16501 caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc
    16561 ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta
    16621 cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag
    16681 gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc
    16741 atatgattac tattgacggt aatggcgcgg ttgcttcggt ggcgtttcgc accagtgaag
    16801 ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg
    16861 ccggaaacgg cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg
    16921 aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt
    16981 ttacgtcatc gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac
    17041 tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctctctattt
    17101 ttggcgatca ttccgacgtc atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg
    17161 ctaacgtcca ggaagcgcaa gattttgctc tcatttcgca catcgcaacg ctgaagagcc
    17221 gcgtgccatt tattcatttc tttgatggtt tccgcacgtc ccacgaaatc aataaaattg
    17281 tcccgctggc cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc
    17341 gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca
    17401 cttatttcca gtctcgcgaa gccaccaacc catggtacga cgcggtctat gaccatgttg
    17461 aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg tttgaatatt
    17521 acgggcatcc gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg
    17581 aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgcc
    17641 tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg
    17701 tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg
    17761 taatgaccgc actggcagaa gcctttaata atggcgagcg cgaaactctg ccccgtgtca
    17821 ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat
    17881 ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt tacgatgatg
    17941 tgaccaatct gtcactgccg ttgccggaaa acaccctgcc aaactcggcg aaactggaag
    18001 ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac aatatcaaga
    18061 ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaagg
    18121 cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc
    18181 tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga
    18241 tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac agcgcagatg
    18301 aagtgtggtc gcgcttgccg caggaagttc aggccgtgct aaatcagaaa aaagcgcgct
    18361 tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata
    18421 ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg
    18481 cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggccaggat ctggtggaac
    18541 gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg
    18601 taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga
    18661 aaaccgtaac cgcagcgatg ctcgcagggc ttggtgatgc cctccccgtt tcggcgctgc
    18721 cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag
    18781 agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtt gccgcttgcc
    18841 cacactcagc tattcgcgca aaagtggtgc cgcctgaagc gatggaaaac gcccctgcca
    18901 gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg
    18961 tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc
    19021 gtcagaatcc agagattaaa gccatcaata tgatgtcgcg cctggaacat gtcgaagaag
    19081 agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac
    19141 gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgct
    19201 ccggttgtgg cgagacgccg tatattaaat tactgactca gctctatggc gaccggatgt
    19261 tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt
    19321 ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg
    19381 ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc
    19441 tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg
    19501 ctacgccaga ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg
    19561 ccgaagcaca tgaactgcta cgtgatgcag atgcactggt ggaaaaatca atctggctga
    19621 ttggtggtga tggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt
    19681 tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc
    19741 aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac ggcaaacgta
    19801 aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga
    19861 tttctctcgg cgcgcagctc aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc
    19921 cggggccatc gctgatcatc gcttatagcc catgtgaaga gcatggttac gatctggcac
    19981 tcagccacga ccagatgcgc caactcacag ctaccggctt ctggccgcta tatcgcttcg
    20041 atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag
    20101 tagcactgga agaaacgtta cttcacgagc aacgtttccg tcggctgaat tcgcagcagc
    20161 cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa cgctatgact
    20221 tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa
    20281 ggattatcca attctaaaaa aaagcccgga cgactgttcg ggcttgtctt tttatatgtt
    20341 ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca ataaaggcat
    20401 ataacccgcg cagaataacg tatagaaaat taaaagtata atttttattt tgtatgtatt
    20461 atcttcgata cattcaccag acttattatc atttcagagg aattatttga ttaaggtttt
    20521 acttaaggcg taacaaatga tatttatcgg ctaactgaac ttctccttta ggatgttttc
    20581 actccccgaa atggggataa aaggcaaata aaataactaa aggatttatt caatgaaaag
    20641 caaagtactg gcacttttaa ttcctgccct gctcgccgca ggtgctgcac atgcagccga
    20701 agtttataat aaagacggca acaaattaga tctgtatggc aaagttgatg gcctgcatta
    20761 tttttctgat aattcggcga aagatggcga ccagagctat gcgcgtctgg gttttaaagg
    20821 cgaaacccaa attaacgatc aactcactgg ctacggtcaa tgggaataca atattcaggc
    20881 aaacaacact gaatcttcaa aaaaccagtc atggacccgt ctggcatttg ccgggctgaa
    20941 atttgcagat tacggttctt tcgattacgg acgtaattat ggcgtaatgt acgacatcga
    21001 aggctggacc gatatgctgc ctgaatttgg cggtgactct tataccaatg cagacaactt
    21061 tatgactggt cgagccaatg gcgtcgcgac ttatcgtaat actgatttct tcggtctggt
    21121 aaatggtctg aacttcgcgg tgcagtatca aggtaacaac gaaggtgcca gtaatggtca
    21181 ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac ggtgacggct ggggtctttc
    21241 cacaacatat gatttaggca tgggctttag cgctggtgcg gcatacacct cttctgaccg
    21301 caccaatgat caggttaacc atactgcggc gggtggtgat aaagcagacg cgtggactgc
    21361 tgggctaaaa tacgatgcta acaatattta cctggcaacc atgtattcag aaacgcgtaa
    21421 tatgaccccg tttggcgaca gcgattatgc tgttgcaaac aaaacccaga actttgaagt
    21481 cactgcacag taccagtttg attttggtct gcgtccggca gtctctttcc tgatgtctaa
    21541 aggccgtgac ctgcacgctg ccggtggtgc agacaacccg gcaggtgttg atgataaaga
    21601 tctggttaaa tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccactta
    21661 tgttgactat aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaatggcat
    21721 ctctaccgat gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc
    21781 tgttatggcg ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac
    21841 tccgtcatta cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga
    21901 ggttttatga acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa
    21961 cgcgtgatta gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg
    22021 acggtaatac cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta
    22081 ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa
    22141 tttaaactgc caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc
    22201 attctggaaa tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg
    22261 gatatcacca cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc
    22321 tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat
    22381 ttcgcaaatg tgctgacatt ttcccctcta atccgtacca tacacgccac tgtttttata
    22441 tcagatttct tatgctgggc attccggcat gacgcatact cttctgatgc catataacga
    22501 attgagtcgc ttttaaatgt cgcaaaatca agaaattagt aagaaagaac aatacaacct
    22561 gaacaagtca gagtaaaaac cgcaactatc tgacgaacaa taacctttat gtttttcaat
    22621 atgttaggca ggattaacaa ctacaataaa gcacaataat ctacactttt tttgccccat
    22681 ttttgcccct tttgcggcct tttgtcccat ttttgccaca gtaaaaatct ataactaatc
    22741 aatctggcgc ccatgatttc tttccaatag aatcagttcc ggctggaaat cattagatac
    22801 tcgcactatc gaaaagctta ccagccaacc gcggcacgtt cttgcatacg aggtgccgcg
    22861 ttttacatgc cgtaccgaga attaatttca ttttacgaaa agttaacccg ccattgctac
    22921 cttaattttt taaaacgaag ttacctctgt cagggtcaaa agcacaatta tctcttccta
    22981 caatcattga aactgcgatt gattccctgc tcaaaggatg agccgccctt gttttaacaa
    23041 gatgtattaa tgccatttta tcatacaaca tacaaacatt tgaattcatt gaattcctca
    23101 taaatacccc atcttctggt tcacataatg taacagggca tgttcgtaag ctctctggca
    23161 cttgcaattg tgcatgctcc acattaaatg cacatacttc aattttatct aacaactcct
    23221 ctaactggta gttagaaagc aaatccatat tttcgagcat ctcaatttca cgcagtactg
    23281 taacttcacg gcttgtcaaa ccaccatgat taagtgtacg ttcaatatgt tctatatatt
    23341 gaaggccaat ctggatatta tcattcgata aaccatcctg gttaagactt cccataaaat
    23401 atccatttac ctgcataggt cgtattgaca ccatcaactc acccagtctt atttgagctt
    23461 ctccacgaat agtctgatta cgtatctcag acaaggtttc ttgaggaatg tcagacaaaa
    23521 aactaacccc aggtaaaata taaggcgtta aatctacagg cattcctctc cctccatttt
    23581 aataacttaa ttttgcttta gtaaaaaata aaagtacata aaactatatt tatatcatta
    23641 attatcactt acaacaaaaa gcttctcttt ttgcatcaaa gtgacattcg tcttttctca
    23701 taatcattga ttctgttata ggttctcgac tcagaggatg ggctccacca gtttcaacaa
    23761 gttgcactaa cgcgtcctta tcatatagag agcatatctc agcacctcgt gagtttctca
    23821 tgaacacccc agtctcaggt gtgtccagcg ttatcgggca tgtcaaaaat gactccgggc
    23881 aagaaaagtg attagaatca ggtctaaata aacaagaatt tattttttcc attaatattt
    23941 tattacttgt tttattttct ctgtgttctg ccatgacact ctgaaaatag ctattaacac
    24001 tacttaaaaa atcgacacct ccatttaatt gcctttccag agccacagcc cttctctcca
    24061 ttcgatgctc acgccccaat agcctatcca gtaagcctcc tctcaccggt tcaactgaaa
    24121 atccatccag tagttggaca tagtgaacag aatattggcc actaccaatg gatattctta
    24181 ttccactttc aagtctggca gcatcctgca aagactgtac tcctgcagga gataattgag
    24241 atatacttgg gatacttaag gtggtaacag gcataaaaac ctcattcagt aattcatatc
    24301 agtttgcagg atgtttcatt aaataaaata aagggtaatg ttcatttcaa ttacccttta
    24361 taacgaagtt tcctctggta tcgtcataaa tgcattcttc atgttttaca attattgatg
    24421 ccgttattgg ttcccgggtc agtgggtggg gtaagccttc accaaccaaa cgagaaaatg
    24481 aagcggcatc aaataaagta catacatctg aaccatctga attcttcaca aaaatacctt
    24541 tttcaggctg ctccagtgta attggacatt gaagcacctc accaggaaag tgaagttttt
    24601 caatatcaac agtaaaggta cattccgata ttttggcctg caaagagttc tcttgtcgtt
    24661 gcgccaccat tccaggaagc acgcttaaca ccctgtttct gagcgcatca actccatcat
    24721 taaaaccaat attcaataat tctgcataaa ccagtcccag cccttcactt tctgctaaaa
    24781 aacggcctgt atctggcata taagaaactc taactctctc ccctcccagg agaaatccac
    24841 caatctgccc ttccattacc tgctgacgca tctccgccat ctcattcgcg aatgaagtcg
    24901 atgatatata tgatactaat ccaggcatta ccatattcct tcatacagat aaatatttcc
    24961 tctgggcttc ataacaaaca tctccctgac atgacaacaa aaaccggagc cggactccgg
    25021 tttttgtgaa tccgtcggct atttcatccc gccaatat
//
LOCUS       DAHBHC010000076        24132 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHBHC010000076 DAHBHC010000000
VERSION     DAHBHC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.076"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="M2A35_003702"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="M2A35_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCB7199948.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATLGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="M2A35_003703"
     CDS             1828..3147
                     /locus_tag="M2A35_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCB7199949.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="M2A35_003704"
     CDS             3445..3486
                     /locus_tag="M2A35_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7199950.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4906
                     /gene="dosC"
                     /locus_tag="M2A35_003705"
     CDS             3524..4906
                     /gene="dosC"
                     /locus_tag="M2A35_003705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCB7199951.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4931..7330
                     /gene="dosP"
                     /locus_tag="M2A35_003706"
                     /gene_synonym="pdeO"
     CDS             4931..7330
                     /gene="dosP"
                     /locus_tag="M2A35_003706"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCB7199952.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7588..8169
                     /gene="ddpX"
                     /locus_tag="M2A35_003707"
     CDS             7588..8169
                     /gene="ddpX"
                     /locus_tag="M2A35_003707"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCB7199953.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8183..9742
                     /locus_tag="M2A35_003708"
     CDS             8183..9742
                     /locus_tag="M2A35_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCB7199954.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVESDLASSWKASDDQKEWTFTL
                     KDNAKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFA
                     PFLYTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNP
                     HYPGNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYP
                     SLRVTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYD
                     ATAMQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKL
                     EKLANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEV
                     DKLLRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPML
                     EQVFNINTMSK"
     gene            9744..10766
                     /locus_tag="M2A35_003709"
     CDS             9744..10766
                     /locus_tag="M2A35_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7199955.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10763..11659
                     /locus_tag="M2A35_003710"
     CDS             10763..11659
                     /locus_tag="M2A35_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCB7199956.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHSFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11656..12642
                     /locus_tag="M2A35_003711"
     CDS             11656..12642
                     /locus_tag="M2A35_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7199957.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQLLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12635..13561
                     /locus_tag="M2A35_003712"
     CDS             12635..13561
                     /locus_tag="M2A35_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7199958.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(13617..14048)
                     /gene="osmC"
                     /locus_tag="M2A35_003713"
     CDS             complement(13617..14048)
                     /gene="osmC"
                     /locus_tag="M2A35_003713"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCB7199959.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14394..14609
                     /gene="bdm"
                     /locus_tag="M2A35_003714"
     CDS             14394..14609
                     /gene="bdm"
                     /locus_tag="M2A35_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCB7199960.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14711..14848
                     /gene="sra"
                     /locus_tag="M2A35_003715"
     CDS             14711..14848
                     /gene="sra"
                     /locus_tag="M2A35_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCB7199961.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15005..16702
                     /gene="maeA"
                     /locus_tag="M2A35_003716"
     CDS             15005..16702
                     /gene="maeA"
                     /locus_tag="M2A35_003716"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCB7199962.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16836..17846
                     /gene="adhP"
                     /locus_tag="M2A35_003717"
     CDS             16836..17846
                     /gene="adhP"
                     /locus_tag="M2A35_003717"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCB7199963.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIVIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            17993..18277
                     /locus_tag="M2A35_003718"
     CDS             17993..18277
                     /locus_tag="M2A35_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCB7199964.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18328..18981)
                     /gene="fdnI"
                     /locus_tag="M2A35_003719"
     CDS             complement(18328..18981)
                     /gene="fdnI"
                     /locus_tag="M2A35_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCB7199965.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIIEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(18974..19858)
                     /gene="fdnH"
                     /locus_tag="M2A35_003720"
     CDS             complement(18974..19858)
                     /gene="fdnH"
                     /locus_tag="M2A35_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCB7199966.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(19871..22918)
                     /gene="fdnG"
                     /locus_tag="M2A35_003721"
     CDS             complement(19871..22918)
                     /gene="fdnG"
                     /locus_tag="M2A35_003721"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(22331..22333),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCB7199967.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23150..24031
                     /gene="yddG"
                     /locus_tag="M2A35_003722"
     CDS             23150..24031
                     /gene="yddG"
                     /locus_tag="M2A35_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCB7199968.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5908 a         6130 c         6096 g         5998 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa cattgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ttggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttatctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg tgtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat agtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaattg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgcgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgcc gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatacggag tcaccgggtt cactgctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggtgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcgtgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattct gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgctatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa atcctgctgg attctgattt
     4141 aggcagtagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtagg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggaatttg aataacagaa gtttgcgggt
     4321 gaagtttcta ttacagataa gaaataccgt atcgcaaatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca acgtccgcag
     4621 tagtgattat gttttccgct acgggggcga tgaatttatc attgttttga ctgaagcttc
     4681 tgaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga aaaccaaact
     4741 gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg ccatgtttaa
     4801 tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt atatcgccaa
     4861 aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc gccaggatgc
     4921 agaggtaatc atgaagctaa ccgatgcgga taatgccgcc gatggcattt ttttccccgc
     4981 ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aatgatgaag tgatgttttt
     5041 caaccccgcc gcagagaagc tctgggggta caaacgtgaa gaagtcattg gcaataacat
     5101 tgatatgctg attccgcggg atttgcgtcc tgcgcatcct gaatacattc gtcataaccg
     5161 tgaaggcggt aaagcgcgtg tcgaggggat gagtcgggag ctgcagctgg agaaaaaaga
     5221 cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg ggaaagttta
     5281 ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac agactcgaca
     5341 attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc cggaacgcca
     5401 tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta gcgaagccag
     5461 cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata accgcattcg
     5521 tttacaacag ttgctatgga aaaccgcccg cgatcaggat gaatttctgc tgttgacgcg
     5581 caccggtgaa aaaatctgga ttaaagcctc tatcagcccg gtttatgacg tgctcgcgca
     5641 tctgcagaac ctggtaatga ccttctcgga tatcaccgaa gaacggcaaa tccgccagct
     5701 tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa tgggggaaat
     5761 catttgtcgt aacatcgaat ctgttctcaa cgaatcgcat gtttcgctgt tcgcactgcg
     5821 caacgggatg ccgatacact gggcgtcatc ttcgcacggt gcagaagttc aaaatgcgca
     5881 aagctggtca gcgaccattc gtcagcgtga tggcgcgcct gcggggatcc tgcaaattaa
     5941 aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata tcagtcagca
     6001 tatggccgcg ctggcgctgg aacaggaaaa aagccgccag catattgaac aactcatcca
     6061 atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc tcgatgacct
     6121 ggtcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata ttcaggatgt
     6181 gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg tcaatcgctt
     6241 tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc agtttgtcct
     6301 cgtgagcctg gaaaacgacg tcagtaacat tacccaaatc gccgatgagc tacggaatgt
     6361 ggtcagcaag ccgataatga ttgacgataa acccttcccg cttaccttga gtattggcat
     6421 cagctacgac gtgggtaaaa accgcgatta cctgctctcc actgctcaca atgcaatgga
     6481 ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga tgaacgaaat
     6541 ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca ataaccaact
     6601 gaaacttgtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg gcatcgaagc
     6661 ccttgcgcgc tggcacgatc cccagcatgg tcatgtgccc ccttcacggt ttattcctct
     6721 cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg
     6781 tcagttagca gaatggcgta gccagaatat tcatatcccg gcgctttccg ttaacttgtc
     6841 ggcactgcac tttcgcagta atcaactgcc taatcaggtg tctgatgcaa tgcacgcctg
     6901 gggtattgac ggccaccagc tgacggtaga aatcacggaa agcatgatga tggaacacga
     6961 taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggct tatcggtaga
     7021 tgattttggt acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat
     7081 caaaattgac aaaagttttg tcgatcgttg tctgaccgaa aaacgcatcc ttgccttact
     7141 tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga
     7201 aaccaaagag caatttgaga tgctacgcaa gatccactgt cgcgttattc agggatattt
     7261 cttttcccgc cccctacccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct
     7321 gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcatttaacg tgacactgtc
     7381 acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac ttcccctctt
     7441 gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa
     7501 gccagcattc tggcgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc
     7561 aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc
     7621 gtaatcttcc ctgatctgga gatcgaattg aaatacgcct gcgctgataa catcacaggt
     7681 aaagctattt atcagcaagc gcgttgtctg ctacacaagg atgcgattac cgcgctggcg
     7741 aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc
     7801 ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat
     7861 gtgacggtcg gttctaatca cagccgtggc acggcgatcg acctgacgct tcgtgatgag
     7921 cacgggaaca tcctcgatat gggcgcaggg ttcgatgaaa tgcacgagcg ctcccatgcc
     7981 tatcaccctt ccgtcccgcc cgccgctcag cgcaatcggc tgttgctgaa tgcgataatg
     8041 actggtggcg gttttgtcgg tatctccagc gaatggtggc acttcgaatt accccaggca
     8101 gcgagttacc ctctgctcgc tgatcaattc acctgtttta tatcccctgg cacacaacac
     8161 gtcagttaat caggaaactg tcatgaagag atcgataccg tttcgtccca cattgctcgc
     8221 gctcgtcctt gccacaaatt tcccggttgc gcacgccgcc gtaccgaaag atatgctggt
     8281 gattggtaag gccgccgatc cacaaaccct cgacccggcg gtaacaatag ataatataga
     8341 taataacgac tggacggtga cctacccgtc ttatcagcgg ctggttcagt acaaaacgga
     8401 cggtgataaa ggctcaaccg acgttgaaag cgatctggca agtagctgga aagcgtctga
     8461 cgatcaaaaa gagtggacgt tcaccctgaa ggacaatgct aaatttgccg atggcacacc
     8521 ggtcactgcc gaagcagtaa aactctcttt tgagcggcta ctaaaaatcg gccaagggcc
     8581 agcagaagca tttcccaaag atttaaagat tgatgctccc gacgaacata cggtgaagtt
     8641 tacccttagc cagccattcg caccgttcct ctacacgctg gcgaatgacg gtgcatccat
     8701 tatcaatccg gcggtcttaa aggaacatgc agcggatgat gctcgcggct tcctcgcgca
     8761 aaataccgcc ggttccggac catttatgct gaaaagctgg caaaaaggtc agcaattagt
     8821 tctggtgcca aatccgcatt accccggcaa taaaccgaac tttaagcgag tatcggtaaa
     8881 aattatcggt gaaagtgcct cccgtcgcct gcagctctcc cgtggcgaca ttgacattgc
     8941 cgatgcgctg ccggtggatc aactcaacgc cctgaagcag gaaaataaag tcaatgtggc
     9001 agagtatccg tcactgcgcg ttacctatct gtatctgaat aacagcaaag cgcctcttaa
     9061 tcaggcggat ctgcgtcggg ccatttcctg gtctaccgat tatcagggca tggttaacgg
     9121 cattctgagt ggtaacggaa aacagatgcg cggcccgatt ccggaaggca tgtggggcta
     9181 cgatgcgacg gcaatgcaat acaaccatga cgaaacgaaa gccaaagccg aatgggataa
     9241 agtgacgagc aaacccacca gcctgacgtt tctctactcc gataacgatc cgaactggga
     9301 gcctattgct ttggcgacac aatccagtct caacaagctg ggcatcaatg tgaagctgga
     9361 aaagctggcg aacgccacca tgcgcgacag agtgggtaaa ggtgattacg acattgcgat
     9421 tggcaactgg agtccggatt ttgccgaccc gtatatgttt atgaattact ggtttgagtc
     9481 agacaaaaaa ggtctgccgg gtaaccgctc gttctatgaa aacagtgagg tcgataagtt
     9541 actgcgcaat gcgcttgcga ccaccgacca gacgcagcgt acccgggact accagcaggc
     9601 acagaaaatc gtcattgatg acgctgctta tgtgtacctg ttccagaaaa actaccaact
     9661 ggcgatgaac aaagaggtga aaggctttgt gttcaatccc atgctggaac aggtcttcaa
     9721 tatcaatacc atgagtaaat aacgtgacct tctggagtat tttacgccaa cgctgctggg
     9781 ggctggtgct cgtggtggcg ggcgtctgcg tgattacgtt tattatctcg catctgatcc
     9841 ctggcgatcc ggcgcggtta ctggcgggtg accgcgccag cgatgctatc gtggaaaata
     9901 ttcgccagca actgggactg gaccagccac tgtatgtaca gttttaccgc tacgtcagcg
     9961 acctgtttca tggtgacctg ggaacatcca ttcgtaccgg gcgtccggtg ctggaagagt
    10021 tgcgtatatt tttcccggcg acgctggagc tggcttttgg tgccctgctg ctggcactcc
    10081 tgattggcat cccgctgggc atactctctg cagtctggcg aaatcgctgg ctggatcatc
    10141 tggtgcgaat aatggccatt accggaatct ccacacctgc gttctggctt gggctgggcg
    10201 tcattgtgct gttttatggt catctgcaaa ttcttcccgg cggcggaagg cttgatgact
    10261 ggctggatcc accaacgcac gttaccggct tttatctgct cgatgcgctg cttgaaggca
    10321 acggtgaagt cttcttcaat gcgttgcaac atctcatctt accggcatta acgctggcgt
    10381 tcgttcacct gggaattgtc gctcgccaga tccgctcagc gatgctggaa caattgagtg
    10441 aagactacat tcgtaccgcc cgggccagcg gcttgcccgg ctggtatatc gttttatgtt
    10501 atgcgctacc caatgcgttg atcccatcga ttaccgtatt gggtttggcg ctgggcgatt
    10561 tgttgtatgg cgcagtgctc accgaaaccg tttttgcctg gcccggaatg ggtgcatggg
    10621 tagtaacatc aatacaggcg ctcgacttcc cggcagtgat gggctttgcc gtcgtggttt
    10681 catttgctta tgtgctggtc aacctggtgg tggatttgct ctatttgtgg attgatccgc
    10741 gtatcggacg tggaggtggt gaatgatgct aagcgaggaa acgtccgccg tacgcccaca
    10801 aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg ctgaaaggca gtccgctcac
    10861 cgtgaccggc gcagtcatca ttgtattaat gctattgatg atgatttttt caccgtggct
    10921 ggcgacgcat gatcccaacg ccattgattt aaccgcccgc cttttgccgc cttctgcggc
    10981 gcactcgttt ggcaccgatg aagtgggacg cgatctgttc agccgcgtac tggtcggcag
    11041 tcagcaatca attctcgccg gattagtcgt ggttgccatt gcgggtatga ttggttcgct
    11101 actcggatgt ctatccggtg tgcttggcgg acgcgcagac gccatcatca tgcgcatcat
    11161 ggacattatg ctgtcgattc cttcgttggt actgacaatg gcactggctg ccgctctcgg
    11221 gccgagttta tttaacgcca tgctggcgat tgctattgtg cgaattccct tttatgtgcg
    11281 cctggcgcgg gggcaagcat tagttgtacg ccagtatacc tatgttcagg cggcgaaaac
    11341 ctttggtgcg tcgcgttggc atctgatcag ctggcatatt ttacgtaact ccctgccgcc
    11401 gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt ttaatggccg ccacgttggg
    11461 atttattggc ctgggtgctc aacaaccgag tgctgaatgg ggcgcgatgg tagcgaatgg
    11521 tcgcaactat gtactcgatc agtggtggta ttgcgcattt ccgggggcag cgattttgct
    11581 taccgccgtt gggtttaatc tctttggtga tggtattcgc gatctgcttg acccgaaagc
    11641 aggaggaaag cagtcatgac ccaacccgtt ctggacattc aacagctgca tttgagtttc
    11701 cccggtttta acggtgatgt tcacgcgctc aacaatgtgt ccttgcagat taaccgcggc
    11761 gaaattgtcg gtctggtggg agaatccggc tcaggtaaat cagtcaccgc aatgctgatt
    11821 atgcgtctgc tacagacggg cagttattgc gtacatcggg ggcagatttc actgctggga
    11881 gacgatgttc ttaacgctcg ggaaaaacag cttcgtcagt ggcgcggcgc acgagtggcg
    11941 atgatctttc aggaaccgat gaccgccctc aatccgacac gtcgaatagg tcttcagatg
    12001 atggacgtga tccgccatca tcaaccaata agtcgtcggg aagccagagc taaagcgatt
    12061 gccctgctgg aagagatgca aatcccggat gccgtggaag ttatgtcgcg ctatccgttt
    12121 gagctttcag gtggtatgcg ccagcgggta atgattgcgc tggcattctc ctgcgagccg
    12181 caattgatta ttgccgacga accgactacg gcgctggacg tcacgctaca gttgcagcta
    12241 ctgcgtttgc ttaaacataa agcccgcgcc agtggaactg cggtactgtt catcagccat
    12301 gatatggtcg tggtgtcgca actgtgcgat agcgtttacg tgatgtatgc cggaagcgta
    12361 attgaaagcg gcgtgacggc agacattatc catcatcccc ggcatccgta taccattggt
    12421 ttgctgcaat gcgcaccaga acatggagta ccacgccagc cattacccgc cattccaggg
    12481 acggtaccaa acctcaccca tttgcctgaa ggctgcgctt ttcgcgatcg ttgctatgcg
    12541 gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt gtggtgacaa caaccagcgc
    12601 tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg
    12661 cgacgtccat atcaatttcc cggcccgtaa aaactggctt ggtaaaacta cggaacatgt
    12721 tcatgccatt aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg
    12781 cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag
    12841 ccacgggcag tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc
    12901 gctctcttcg cttaatccgc gcttaccggt gtggcgcatc atcacagaac cgctctggat
    12961 agctaagcgt agtagtgaac aacagcggcg agcgttggca gaggagctgg ctgtgcaggt
    13021 gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca
    13081 acgtatcgcc attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc
    13141 aacctctgcg ctggatatct ccgtgcaggc gcagatcctc aatttactgg taacgctaca
    13201 ggaaaatcac gggctgacct atgtgctgat ttcacacaat gtctcggtga tacgtcatat
    13261 gagcgatcgg gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca
    13321 ggtgctaacg gctccggcac atccatacac ccgattattg ctggattccc tccccgccat
    13381 tgataaaccg ctggaggaag aatgggcatt acgtaaaacg gatctgccgg gaaaccgcac
    13441 gttgccgcaa ggctgttttt tctacgaacg ttgccccctt gcaaccagcg gatgtgaagt
    13501 ccggcaatca ttagcaataa gggaggacgg acgtgagctc cggtgctggc gggcgctgta
    13561 gagatgctaa caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac
    13621 gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagaaac cgggcatcct
    13681 gcttttgctt tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact
    13741 tcactcttca gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca
    13801 tcggcggtgg tatcaatcga tgttggcgta aatcccgctt cccccagcat taatgaaagc
    13861 gccattgaga aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt agttcctttt
    13921 tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt tcagcacacc actctcggtg
    13981 gatactgttc ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg
    14041 attgtcattg ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag
    14101 cacgaggata agcaggatat tccgaataaa aatccggcag aaatattact tctgcactta
    14161 aattaaattt ccacaataga taaccgggaa tatataaccc aagagatatt gcattaatat
    14221 taatcaactc accacaataa taatacctat caatatcatg aggttaaaat ataaaaatca
    14281 tcaaccagga ctaatcttaa caacgaagcg gcaaatattt gccatgctga atgtgcttta
    14341 taaatctgcg atccgtagca gacaccataa atacacagac acggagaatc actatgttta
    14401 cttattatca ggcagaaaat tcaacagcag aacccgctct ggttaatgcc attgagcaag
    14461 gccttcgggc agagcacggt gttgtcactg aagatgacat tctcatggag ctgaccaaat
    14521 gggtggaagc ttctgataac gacatcctca gtgatatcta ccagcaaacg atcaattatg
    14581 tggtcagtgg ccagcaccct acgctttaag gtgctatgct tgatcggcaa cctaatttag
    14641 gggtttagca cgtgtttctt cgctacggcg atgttgtcct taaaactagc aacaggattg
    14701 aggagttaaa atgaaatcga accgtcaggc acgtcatatt cttggactgg accataaaat
    14761 ttctaaccag cgcaaaatag ttaccgaagg tgacaaatcc agcgtggtaa ataatccaac
    14821 cggcagaaaa cgccccgctg aaaagtaatt cataaccatc agtcctcaat gacgattaaa
    14881 caccattgcc tgcgcaatgg tgtttttgtt tttatctgct ttatacttgg ggccgacgcc
    14941 ctggcggtaa agcaaagacg ataaaagcgt gccagggatg gatattcaaa aaagagtgag
    15001 tgacatggaa ccaaaaacaa aaaaacagcg ttcgctttat atcccttacg ctggccctgt
    15061 actgctggaa tttccgttgt tgaataaagg cagtgccttc agcatggaag aacgccgtaa
    15121 cttcaatctg ctggggttac tgccggaagt ggtcgaaacc atcgaagaac aagcggaacg
    15181 agcatggatc cagtatcagg gattcaaaac cgaaatcgac aaacacatct acctgcgtaa
    15241 catccaggac accaacgaaa ccctcttcta ccgtctggta aacaatcatc ttgatgagat
    15301 gatgcctgtt atttataccc caaccgtcgg cgcagcctgt gagcgttttt ctgagatcta
    15361 ccgccgttca cgcggcgtgt ttatctctta ccagaaccgc cacaatatgg acgatattct
    15421 gcaaaacgta ccaaaccata atatcaaagt gattgtggtg actgacggtg aacgtattct
    15481 ggggcttggt gaccagggca tcggcgggat gggcattccg atcggtaaac tgtcgctcta
    15541 taccgcctgt ggcggcatca gcccggcgta tacccttccg gtggtgctgg atgtcggaac
    15601 gaacaaccaa cagttgctta acgatccgct gtatatgggc tggcgtaatc cgcgtatcac
    15661 tgacgacgaa tactatgaat tcgttgatga atttatccag gctgtgaaac aacgctggcc
    15721 ggacgtgctg ttgcagtttg aagacttcgc acaaaaaaat gcgatgccgt tacttaaccg
    15781 ctatcgcaat gaaatttgtt cctttaacga tgacattcag ggcactgcgg cggtaacagt
    15841 cggcaccctg atcgcagcaa gccgcgcggc aggtggtcag ttaagcgaga aaaaaatcgt
    15901 cttccttggc gcaggttcag cgggatgcgg cattgccgaa atgatcatcg cccagaccca
    15961 gcgcgaagga ttaagcgagg aagcggcgcg gcagaaagtc tttatggtcg atcgctttgg
    16021 cttgctgacc gacaagatgc cgaacctgct gcctttccag accaaactgg tgcagaagcg
    16081 cgaaaacctc agtgactggg ataccgacag cgatgtgctg tcactgctgg atgtggtgcg
    16141 caatgtaaaa ccagatattc tgattggcgt ctcaggacag accgggctgt ttacggaaga
    16201 gatcatccgt gagatgcata aacactgtcc gcgtccgatc gtgatgccgc tgtccaaccc
    16261 gacgtcacgc gtggaagcca caccgcagga cattatcgcc tggactgaag gtaacgcgct
    16321 ggtcgccact ggcagtccgt ttaatccagt ggtatggaaa gataaaatct accctatcgc
    16381 ccagtgtaac aacgccttta ttttcccagg catcgggctg ggtgttattg cttccggcgc
    16441 gtcacgtatc accgatgaga tgctgatgtc ggcaagtgaa acgcttgctc agtattcgcc
    16501 gctggtcctg aacggcgaag gtctggtact accggaactg aaagatattc agaaagtctc
    16561 ccgcgcaatt gcgtttgcgg ttggcaaaat ggcgcagcag caaggcgtgg cggtgaaaac
    16621 ctctgccgaa gccctgcaac aggccattga cgataatttc tggcaagccg aataccgcga
    16681 ctaccgccgt acctccatct aagcctgcgc ccggtagtga aggctaccgg gctatttccc
    16741 tctccctttt tcagatctca tccatactgg gtagtggcga ataaatctca tttgcctcac
    16801 ctgctatgca gaacatcatc cgaaaaggag gaactatgaa ggctgcagtt gttacgaagg
    16861 atcatcatgt tgacgttacg gataaaacac tgcgctcact gaaacatggc gaagccctgc
    16921 tgaaaatgga gtgttgtggt gtatgtcata ccgatcttca tgttaagaat ggcgattttg
    16981 gtgacaaaac cggcgtaatt ctgggccatg aaggcatcgg tgtggtggca gaagttggcc
    17041 caggtgtcac ctcattaaaa ccaggcgatc gtgccagcgt ggcgtggttc tacgaaggat
    17101 gcggtcattg cgaatactgt aacagtggta acgaaacgct ctgccgttca gttaaaaatg
    17161 ccggatacag cgttgatggc gggatggcgg aagagtgcat cgtggtcgcc gattacgcgg
    17221 taaaagtgcc agatggcctg gactcggcgg cggccagcag cattacctgt gcgggggtca
    17281 ccacctacaa agccgttaaa ctgtcaaaaa ttcgtcccgg tcagtggatt gctatctacg
    17341 gtcttggcgg tctgggtaac ctcgccctgc aatacgcgaa gaatgtcttt aacgcgaaag
    17401 tgatcgtcat tgatgtcaat gatgagcagt taaaactggc aaccgaaatg ggtgcagatt
    17461 tagcgattaa ctcacgcacc gaagacgccg ccaaaattgt gcaggagaaa accggtggcg
    17521 ctcacgctgc ggtggtaaca gcagttgcta aagctgcgtt taactcggca gttgatgctg
    17581 tccgtgcagg cggtcgtgtt gtggctgtcg gtctgccgcc agagtctatg agcctggata
    17641 tcccacgcct tgtgctggat ggcattgaag tggtcggttc gctggtcggc acgcgccagg
    17701 atctaaccga agccttccag tttgccgccg aaggcaaagt ggtgccgaaa gtcgccctgc
    17761 gtccgttagc ggacatcaac accatcttta ccgagatgga agaaggcaaa atccgtggcc
    17821 gtatggtgat tgatttccgc cgttaagagg cctttgctgc gagtgccagt cctggtgtcg
    17881 cagcatcgca ctctctccga cattaaatgg ctaaatggat tattgttcct tcctacgcaa
    17941 caataatgat agtgttcctg atgaaggaac actactgaga gtaaagacac tgatgaaaat
    18001 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
    18061 cctgcgcgag tttgccagag caatggaaat tgctccctca acggcaagcc gattgctgac
    18121 cggaaaagca gctttgacac cagaaatggc gattaaactt tccgtggtga tcggcagttc
    18181 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
    18241 ggatgtgtcg cgactacgcc gtctggttac gcaataaaaa aatggcgctt cttaaagcgc
    18301 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
    18361 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
    18421 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
    18481 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
    18541 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
    18601 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
    18661 accgacatcc gccactttat gctcattgcc tttcaatact tcgataatgt tcaacagcca
    18721 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
    18781 tgcgacgaaa atcgcaatgc cgaaaaacgg gtgcaaaatg cgtcccatct gcggcgtacc
    18841 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
    18901 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
    18961 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
    19021 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
    19081 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
    19141 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
    19201 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
    19261 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
    19321 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
    19381 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
    19441 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
    19501 gaatgattgc accagcagac gggcacgcct tcaggcagcc gggatcttca cagtgcatac
    19561 agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca
    19621 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
    19681 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
    19741 tacaggtgga aacgtcgata agttttgcga cttctgcttt gtaatcacgc acctgagaag
    19801 gcggcgtgat ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc atagccatct
    19861 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
    19921 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
    19981 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgacca tttacattca
    20041 gtggtttcag acgacgcgtt accacagcca ccgcgcggat aaagccacgc ttgcttgaga
    20101 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca
    20161 caaactgttc cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt
    20221 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
    20281 gtgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
    20341 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
    20401 tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggc ccggttggcg
    20461 tacccggtgc ggcattgccg aagtcaggaa tatcgttacc cgtccacttg ctgccgttcc
    20521 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
    20581 tgtacagcac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
    20641 cggacgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg
    20701 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
    20761 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccagcgcat
    20821 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
    20881 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca
    20941 ggtgatggta gatacccgcc agaatttcac cgtcgttacg cgcttcgccc ggcgcgtcct
    21001 gacctttcca gtgccactgc agccagcgac cggagttagc aatagaacca tcttcttcag
    21061 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
    21121 tcgactcgcc gtggttctgc cagaaggttg aggtttcagt caccagcgga tcgataacca
    21181 ccatgtactt cagcttgctt aggcagctca ccactttgtt tttgtccggg aaggacgcaa
    21241 ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc atgttgaaat
    21301 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
    21361 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
    21421 tgctccagta gttcacctga ccagccagcg tcgctttcgg cgtgttcgct tccagatacg
    21481 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
    21541 gcagacctaa gtcagtcaga ccctgaatgt tggagtgacc acgcaatgca ttcacgccgc
    21601 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
    21661 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
    21721 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc
    21781 agatgttttc aacgacgtct ggcgtgtagc gggaaacgtg ctctttcagc aggttccaca
    21841 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
    21901 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
    21961 cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgt ttaacgtatt
    22021 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
    22081 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacgag
    22141 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
    22201 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
    22261 tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg
    22321 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
    22381 atttctgtgt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
    22441 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
    22501 cagctttcat cagctttgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
    22561 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat
    22621 ccagcaaacc ggcccctttt gggcacagcg caccacggct taccggatga tccgggtcac
    22681 cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca
    22741 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
    22801 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
    22861 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
    22921 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
    22981 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
    23041 acctacctca atagcggcag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
    23101 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
    23161 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
    23221 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
    23281 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
    23341 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
    23401 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tatggcccag
    23461 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
    23521 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgata atgggttaca
    23581 ttatgatgaa atcatcaata atattaccac cagcccattg agttatttcc tggcgtttat
    23641 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
    23701 cggaattacc gtttttgtcc tgctaacggg agcaagtctg tgggtttact attttctcac
    23761 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
    23821 taccttagga tttgcttatg ctgcatggaa tgtcggtata ctgcatggca atgtcaccat
    23881 aatggcggta ggctcgtatt ttacgcccgt actttcctca gcgcttgcag ccgtgctgct
    23941 cagcgccccg ctgtcgttct cattctggca aggcgcgcta atggtctgcg gcggttccct
    24001 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
    24061 taatcagata aattccgaca atattaaaaa ataaatttca aatgccagta attcacctta
    24121 tatctcattt cg
//
LOCUS       DAHBHC010000077        23496 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHBHC010000077 DAHBHC010000000
VERSION     DAHBHC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.077"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(69..1157)
                     /locus_tag="M2A35_003723"
     CDS             complement(69..1157)
                     /locus_tag="M2A35_003723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCB7199969.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNTAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(1160..2017)
                     /gene="nfo"
                     /locus_tag="M2A35_003724"
     CDS             complement(1160..2017)
                     /gene="nfo"
                     /locus_tag="M2A35_003724"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCB7199970.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(2091..3140)
                     /locus_tag="M2A35_003725"
     CDS             complement(2091..3140)
                     /locus_tag="M2A35_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCB7199971.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            3239..4120
                     /locus_tag="M2A35_003726"
     CDS             3239..4120
                     /locus_tag="M2A35_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7199972.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            4325..5794
                     /gene="lysP"
                     /locus_tag="M2A35_003727"
     CDS             4325..5794
                     /gene="lysP"
                     /locus_tag="M2A35_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCB7199973.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            6088..8079
                     /gene="cirA"
                     /locus_tag="M2A35_003728"
     CDS             6088..8079
                     /gene="cirA"
                     /locus_tag="M2A35_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCB7199974.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(8111..8947)
                     /gene="yeiG"
                     /locus_tag="M2A35_003729"
     CDS             complement(8111..8947)
                     /gene="yeiG"
                     /locus_tag="M2A35_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCB7199975.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            9205..9873
                     /gene="folE"
                     /locus_tag="M2A35_003730"
     CDS             9205..9873
                     /gene="folE"
                     /locus_tag="M2A35_003730"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCB7199976.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            9890..11047
                     /locus_tag="M2A35_003731"
     CDS             9890..11047
                     /locus_tag="M2A35_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCB7199977.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     RDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISYRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWFWRQLTLRAAGPAISKTSR"
     gene            11189..12229
                     /gene="galS"
                     /locus_tag="M2A35_003732"
     CDS             11189..12229
                     /gene="galS"
                     /locus_tag="M2A35_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCB7199978.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            12509..13507
                     /gene="mglB"
                     /locus_tag="M2A35_003733"
     CDS             12509..13507
                     /gene="mglB"
                     /locus_tag="M2A35_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCB7199979.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            13568..15088
                     /gene="mglA"
                     /locus_tag="M2A35_003734"
     CDS             13568..15088
                     /gene="mglA"
                     /locus_tag="M2A35_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCB7199980.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            15104..16114
                     /gene="mglC"
                     /locus_tag="M2A35_003735"
     CDS             15104..16114
                     /gene="mglC"
                     /locus_tag="M2A35_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCB7199981.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(16357..17592)
                     /gene="preA"
                     /locus_tag="M2A35_003736"
     CDS             complement(16357..17592)
                     /gene="preA"
                     /locus_tag="M2A35_003736"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCB7199982.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(17586..18824)
                     /gene="preT"
                     /locus_tag="M2A35_003737"
     CDS             complement(17586..18824)
                     /gene="preT"
                     /locus_tag="M2A35_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCB7199983.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(19018..19257)
                     /locus_tag="M2A35_003738"
     CDS             complement(19018..19257)
                     /locus_tag="M2A35_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCB7199984.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(19260..19979)
                     /gene="sanA"
                     /locus_tag="M2A35_003739"
     CDS             complement(19260..19979)
                     /gene="sanA"
                     /locus_tag="M2A35_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCB7199985.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(20129..21013)
                     /gene="cdd"
                     /locus_tag="M2A35_003740"
     CDS             complement(20129..21013)
                     /gene="cdd"
                     /locus_tag="M2A35_003740"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCB7199986.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(21143..21838)
                     /locus_tag="M2A35_003741"
     CDS             complement(21143..21838)
                     /locus_tag="M2A35_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCB7199987.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(21835..22233)
                     /locus_tag="M2A35_003742"
     CDS             complement(21835..22233)
                     /locus_tag="M2A35_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCB7199988.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            22472..23419
                     /gene="dusC"
                     /locus_tag="M2A35_003743"
     CDS             22472..23419
                     /gene="dusC"
                     /locus_tag="M2A35_003743"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCB7199989.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         5767 a         5869 c         6119 g         5741 t
ORIGIN      
        1 ttacttgctg aaattgcctg atttgtaggc cggataagac gcgttaagcg tcgcatccgg
       61 caacattgtt acaccattgg cactaatgaa agcgcgttat cggcagacag ggtagggttg
      121 tttaagctgc cgctggcgcg cgaaattgcc gcgcaggcta ccgcaaaacg ggcgctgtcg
      181 cggaaactgt atccttcaag aaagctatat accaggcccg ccataaaacc atcgtcagca
      241 ccaaaactgt ctactgtcgt atgcgctggc gcggtcagca aaaattgttc tccatccttt
      301 tcgctgcaat aaactgactc atcgggcaaa taaacaaaca gttgctgaac accttgctga
      361 tgcaatgcat tcactgcggt attacggtca gcatcgctgg tgatcgcctg tccccataaa
      421 atttccagct ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca gtgtttgatt
      481 ttgcccgctt tgaattctga aacggtatcg acaaacaccg ggatttcatc agcgagggta
      541 aagacccatt ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac gcccgcatga
      601 cgaagtaaat cgcgcgaccc gttcagtagt tgcggtgtca actgttccag cagatgggta
      661 tcgttaatcg ccagcacggt ttgatcgtct cgattggcaa ttgccagata cgtcgatgtg
      721 ctttgaccat gcaaacgaac gcagccggag acattcacgc cggcacggcg cgtttcttcc
      781 aggagcattt cgccataaaa gtcatcgcca atcactgaaa gcaaatggac gtcacggcct
      841 aacagcgcca gattgtgggc gatgttgcgt cccacgccgc ccgctgagca atgaattgta
      901 ccgggatgag aagccgattg cgggtaacgg atatccgcca tcccgcgaat atccatattg
      961 attgtcccca ccactacgca gtattcctgc tcggtgagaa tgtaaccttt gcctttaatc
     1021 cggcctttgc gcattaaatc cataatatgc gccgcaacgc gcgaacggct gatttgcagc
     1081 atgtccgcaa tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc aaggatctcc
     1141 ttttcccggt tattcatctt caggctaccg ctttttcagt ttgttgcgct ttcagccagg
     1201 cgatctcttc tgcccagata tccgggttga tggtttcgag gatcagcgga atgccgtcga
     1261 aacggtcgtc ctgcatgatc cagcggaacg catcatgacc gatattgcct tcaccgaggc
     1321 tatgatggcg gtcaacgcgg ctgccaaagg tgcttttcgc atcgttaagg tgcatcccgc
     1381 gcagatactt aaagccgaca gtacgggcaa aatccgcgaa tgttttctcg cattcggctg
     1441 gagtacgcaa atcatacccg gcagcgaaag catggcaggt atcaatgcag acgccgacgc
     1501 gggatttatc ttccacgccg tcgataatcg ccgcgagatg ttcgaattta aaccctaagt
     1561 tactgccctg accggcggtg ttttctatca ccgctgtcac accttgagtt ttatccagcg
     1621 caatgttgat ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat
     1681 ggctgccagg gtggaagttg agcaaagaaa gccccagctg ttcgcaacgc tgcatttcat
     1741 ctataaaggc atcgcgcgat ttttccagag cttcagtgac cggatgtccg aggttaatca
     1801 gataactgtc gtggggaaga atttgcgccg atgtgtagtg atatttttca caggcggctt
     1861 tgaattcatc gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg
     1921 tgaacaaggc aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca tttgccagac
     1981 cgccagcagc actaacgtgc gctccaatgt atttcatgcg aggactcctg ttaaacccgc
     2041 tggaggaaaa cggtaatgat agcgggttaa caggagcaag atgtagtggt ttatgcgatg
     2101 acgctttgaa tcacatagtt aatcgcacca ccaccaacaa tcagccaggc aaacagtacc
     2161 agtgccatca gcagaggttt cgccccagct tttttcagcg cgctgacgtg agtggtcaga
     2221 cccagcgccg ccatcgccat tgccagcagg aaggtatcca gcgttaccag catgttcacc
     2281 acgctctgcg gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa caagatggca
     2341 aaccacggaa tagtgatttt gcttttctcg ccgctgttcg ccccagacag ctgtttaaca
     2401 cgcgccgcca gcaggatgag gaacggagcc agcatcatca cgcgcagcat tttggaaata
     2461 actgctgcgt tttccgcatc cgggctgatg gcatgacctg ccgccaccac ctgcgccact
     2521 tcgtgcacag tagaaccaat gtagataccg aaagtttccg gactaaacca ttgagacatc
     2581 agcggatata tcgccgggta gaggaaaatc gcgacggtcc cgaagataac aacggttgca
     2641 acagccacgg ttactttact ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg
     2701 gcagcaccac agatactgct accggcaccg atcaaccagc tggtgtgctt atccagacca
     2761 aacactttct gccccaggaa gcaagccagc aggaaggtac tggacagcgt caacacgtca
     2821 atgatgatcc cactgatacc gacatcggca atttgcgaga acgtcagacg gaagccataa
     2881 agaatgatac ccagacgtaa taaatattgc ttggcaaaca gcacaccacc gtcacagctt
     2941 ttccagatgt gcggatagat ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg
     3001 agggcactaa acccggcacc cgcaaccgcg ggaatggaac caccccacag ggcgaccccg
     3061 gtgataactg cactcagggc taaccccgga ataaaatgcc acagtgtacg atgttgtttc
     3121 tgtaaggtga tattcgtcat aaccctctcc tttaaccggg cataaggtta cgactaactg
     3181 gtttaaaaat aaaattgatt atatatttat aattaatctt tataagtggt aagcgactat
     3241 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
     3301 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgaccgacct
     3361 ggaagggcag cttggcgtgc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
     3421 acacgggcgg ctgctctatc cgcgtgcgtt ggcattgctt gaacaggcgg ttgaaatcga
     3481 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
     3541 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
     3601 acttagcgtt gggaatagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
     3661 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
     3721 cgagctggtg gttttcgccg cgccgacttc gccgttggcc cgtggtccgg tcaccttaga
     3781 acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggagattgt
     3841 cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
     3901 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
     3961 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tgccgcgcct
     4021 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
     4081 ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac aagaacatgc
     4141 tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag aaaagagatt
     4201 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     4261 ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg gatagcattt
     4321 ttgtatggtt tccgaaacta aaaccacaga agcgccgggc ttacgccgtg aattaaaggc
     4381 gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc
     4441 tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg
     4501 cctgatggtt tacttcctga tgaccagtct cggtgaactg gctgcatata tgccggtttc
     4561 cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg
     4621 ctggaactac tggtacaact gggcggtgac tatcgccgtt gacctggttg cagctcagct
     4681 ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg
     4741 cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg aatactggtt
     4801 ctcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat
     4861 cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc
     4921 gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca
     4981 gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc
     5041 acgcgcggta cgtcaggtgt tctggcgaat cctgttgttc tatgtgttcg cgatcctgat
     5101 tatcagcctg attattccgt acaccgatcc gagcctgctg cgtaacgatg ttaaagacat
     5161 cagcgttagt ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt
     5221 gatgaacgca gttattctga cggcggtgct gtcagcgggt aactccggta tgtatgcgtc
     5281 tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact
     5341 gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg
     5401 cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctctgg
     5461 gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg
     5521 ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact
     5581 ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc
     5641 gttcctgaaa gatactattg actggggcgg cgtagcggca acgtatattg gtatcccgct
     5701 gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag
     5761 cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct
     5821 ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt
     5881 aacatttgga ttgataattg ttatcgtttg cattatcgtt acgccgcaat caaaaaaggc
     5941 tgacaaatca gaggctgttc cggctttctg ggatgatcac ctgcataaaa aataagtcca
     6001 ccgcgatgct gccgtacgca aggggacgtg aagaagatgt gagcgataac ccattttatt
     6061 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
     6121 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
     6181 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc tgccagtatc
     6241 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
     6301 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
     6361 cgtggtctgg acagcagcta taccctgatt ctcgtcgacg gtaaacgcgt gaactcccgc
     6421 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
     6481 cgtattgaag tggtccgtgg cccgatgtcg tcgctgtacg gttccgatgc gctcggcggt
     6541 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
     6601 accaccattc aggaacatcg cgatcgcggt gacacctata acggtcagtt ctttaccagt
     6661 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
     6721 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
     6781 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
     6841 actgccggat acggtttcga ccgtcaggat cgtgattccg actcgctgga caaaaaccgc
     6901 ctggaacgcc agaactactc cgtcagccat aatgggcgtt gggattacgg caccagcgaa
     6961 ctgaaatact acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
     7021 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
     7081 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
     7141 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
     7201 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
     7261 gaacactgga gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa
     7321 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
     7381 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
     7441 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaaggggtt
     7501 gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
     7561 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
     7621 ggacggcgca taccggtatt tagctactac aacgttaaca aagctcgtat tcagggcgtg
     7681 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
     7741 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
     7801 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
     7861 tctgggcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
     7921 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
     7981 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
     8041 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
     8101 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
     8161 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
     8221 ctttctggcg cgcggcttct gccagtaccg caggttgcaa ctggtcggca agaaactgat
     8281 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
     8341 gtgcgcaact gtcccattcc agccatgcat ttttgtcctc acctaaatag ctgctaaacg
     8401 ctttgattcc ccacgggacg ctgcacggat tcacaattgg cgcaaaggcc gaaacgctgg
     8461 tgtatttacc cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac
     8521 cgctaatggc gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat
     8581 cgcgcagata atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat
     8641 aaaagcctgc gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg
     8701 ggctggtgtc tggcatcacc agtacaatcc ccagttccgc cgctacccgc tgggcacccg
     8761 ccttggtggt gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg
     8821 gcggagtgtg atcacgaggt ggagggagaa agatactgaa cgtcatcggg cagtttaagg
     8881 tactggagtc gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca
     8941 tttccatgcg tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa
     9001 tgtgctgtga gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg
     9061 aaaggcagcg gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca
     9121 cgcacaataa tcaggctgta aatcagctta ataactttgc ccccacgcag ggcggaggcg
     9181 tcacacctgc aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat
     9241 gaagcgttag ttgcgcgagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat
     9301 aacgaaacgc gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat
     9361 ctcgacctgg ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc
     9421 gatgaaattt tctccggtct ggattacgcc aatttcccga aaatcaccct cattgaaaac
     9481 aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag cacctgtgaa
     9541 caccattttg ttaccatcga tggcaaagcg acggtggcct atatcccgaa agattcggtg
     9601 atcggtctgt caaaaattaa ccgcattgtg cagttctttg cccagcgtcc gcaggtgcag
     9661 gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg
     9721 gctgtctcga tcgacgcggt gcattactgc gtgaaggcgc gtggcatccg cgatgcaacc
     9781 agtgccacga caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac
     9841 gagtttctgc gcgctgtgcg tcatcacaac tgattaaaag gcaggaacca tggagcgcaa
     9901 cgtcacgctc gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag
     9961 cgcctttggg ctaccaaagg cggcttatct caatcccgcc tggtacggtg ctattacgcc
    10021 gcgggatgca tggacttggg catttcttga tctcatcggc caggtgaaat tcctcacgct
    10081 ttttgcgcta ctgtttggtg cgggcctgca aatgttgctg ccccgtggca gacgctggat
    10141 ccagtcgcgg ttaacgctgt tagtcttgtt gggctttatt cacggtttat tgttctggga
    10201 cggcgatatt ttgctggctt acgggctggt gggcttaatc tgctggcggc tggtgcgcga
    10261 tgcgccatcg gtaaaaagcc tgtttaatac aggcgtcatg ctttatctgg tggggcttgg
    10321 cgttttgctg ttattggggc tgatttccga cagccagacc agccgcgcct ggacgccgga
    10381 tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg aagcgatcag
    10441 ttatcgtgcc gatggtgttg gcaacagttt actggcactg ggcgcacagt atggctggca
    10501 actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc tgaaagggca
    10561 gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg gggtgaccat
    10621 taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt
    10681 cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct atgcgtcgct
    10741 gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga tcgcctgcgt
    10801 cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca cgctttttta
    10861 ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg ttattccggt
    10921 atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc aggggccggt
    10981 ggaatggttc tggcgtcagt taactttgcg tgctgccgga ccggcaatat ctaaaacatc
    11041 aagataacga tctggatcac attcgttaac aaaacggctg taaccgtttc cattgctgtg
    11101 actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca agcagtcatt
    11161 tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag cgcgtcaggc
    11221 tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg tcagtgccga
    11281 cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa acgccaatgc
    11341 ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg acgtttctga
    11401 tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc gctcagcagc atcagaaata
    11461 cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt
    11521 aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg acgatgaact
    11581 ggcgcaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg tgccggggta
    11641 cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga cgcgcatgtt
    11701 gctgaataac ggtcatcaac gtattggtta tctttcttcc agccacggca ttgaagatga
    11761 cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta ttccgccgga
    11821 aagctggatt ggcgctggta cgccggacat gccgggcggt gaggcggcga tggttgaact
    11881 gctggggcgc aatctacaac ttaccgctgt atttgcttat aacgacaata tggccgctgg
    11941 cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct caatcatcgg
    12001 tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc
    12061 cattgcttca atggcgaaat tagccaccga actggccttg cagggggcag caggcaatat
    12121 tgatcctcgt gccagccact gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac
    12181 acgccagaat gcggcggcga tcactaactc aacaaatcag gcgatgtaac cgctttcaat
    12241 ctgtgagtga tttcacagta tcttaacaat gtgatagcta tgattgcacc gttttaacgt
    12301 tgtaacccgt atgtaacagt gaataatcac ttttgccgag gtaacagcgt cataacaaca
    12361 attaaagccg ttttctggag cgttaccggg catggaagaa cgaattttaa aaagtgagct
    12421 tcggcgttca gtaacacttc attaactcta ctgccccgcc gagcatttat ctcaagcact
    12481 accctgcata agaaaaaccg gagataccat gaataagaag gtgttaaccc tgtctgctgt
    12541 gatggccagc atgttattcg gtgccgctgc acacgctgct gatactcgca ttggtgtaac
    12601 aatctataag tacgacgata actttatgtc tgtagtgcgc aaggctattg agcaagatgc
    12661 gaaagccgcg ccagatgttc agctgctgat gaatgattct cagaatgacc agtccaagca
    12721 gaacgatcag atcgacgtat tgctggcgaa aggggtgaag gcactggcaa tcaacctggt
    12781 tgacccggca gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg tgccggtggt
    12841 tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag cctactacgt
    12901 tggcactgac tccaaagagt ccggcattat tcaaggcgat ttgattgcta aacactgggc
    12961 ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt cagttcgtac tgctgaaagg
    13021 tgaaccgggc catccggatg cagaagcacg taccacttac gtgattaaag aattgaacga
    13081 taaaggcatc aaaactgaac agttacagtt agataccgca atgtgggaca ccgctcaggc
    13141 gaaagataag atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg aagtggttat
    13201 cgccaacaac gatgcgatgg caatgggcgc ggttgaagcg ctgaaagcac acaacaagtc
    13261 cagcattccg gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg tgaaatccgg
    13321 tgcactggcg ggcaccgtac tgaacgatgc taacaaccag gcgaaagcga cctttgatct
    13381 ggcgaaaaac ctggccgatg gtaaaggtgc ggctgatggc accaactgga aaatcgacaa
    13441 caaagtggtc cgcgtacctt atgttggcgt agataaagac aacctggctg aattcagcaa
    13501 gaaataagac tgattgggcg cagtctatta ctgcgccctt attgtatggc caacaaggta
    13561 aaattatatg gtcagctcaa cgactccgtc ctccggggaa tacttgttgg aaatgagcgg
    13621 tatcaacaag tcctttcctg gtgttaaggc acttgataac gttaatttaa aagtccggcc
    13681 acattctatc catgcattaa tgggggaaaa cggtgcagga aaatcgacat tattaaaatg
    13741 cctgtttggt atttatcaaa aagactccgg caccatttta ttccagggta aagagatcga
    13801 tttccattct gcaaaagaag ccctggaaaa tggtatttcg atggtacacc aggagttaaa
    13861 cctggtatta caacgttcgg tgatggataa catgtggctg gggcgatatc ccaccaaagg
    13921 catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg acgaactgga
    13981 tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa tgcagatgat
    14041 cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg attatggatg aaccgacttc
    14101 ttcgttaacc gaaaaagagg tcaatcatct gttcactatt attcgtaaat taaaagagcg
    14161 cggctgcggt attgtttata tctcgcataa aatggaagaa atcttccagt tatgtgatga
    14221 agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag gactgacgat
    14281 ggacaagatc atcgccatga tggttgggcg ttctcttaac cagcgtttcc ctgacaaaga
    14341 aaacaaaccg ggcgaagtca tcctcgaggt acgtaacctg acgtcactgc gccagccgtc
    14401 gattcgcgat gtctcgtttg atctgcataa aggggagatc ctcggtattg ccggtctggt
    14461 gggggcgaaa cgtaccgata ttgttgagac gttatttggt attcgcgaga aatcggctgg
    14521 caccataacg ttgcacggca aacagatcaa taaccataat gccaacgaag ccataaacca
    14581 tggatttgca ctggtaactg aggagcgccg ctcaacggga atttatgcct atctggatat
    14641 tggttttaac tcgttaattt ccaatattcg caactacaaa aataaagttg gtttactgga
    14701 taactcgcgg atgaaaagcg atacccagtg ggtgattgat tcgatgcggg taaaaacgcc
    14761 gggtcatcgg acgcaaattg gttcgctctc cggtggtaat cagcaaaagg tgattattgg
    14821 tcgctggcta ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc gcggtattga
    14881 tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga aaggcaaggg
    14941 gattattatt atctcctctg aaatgcctga gttgttaggg ataacagacc gtattctggt
    15001 catgagcaat ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc aaaacgaaat
    15061 tctgcgtctt gcgtctttgc acctttaaga ttaggggctt cccatgagtg cgttaaataa
    15121 gaaaagtttt cttacttacc tgaaagaggg cggtatttac gtcgttcttt tagttttgct
    15181 ggcgattatt attttccagg acccaacatt tttaagtctg ttgaacttaa gtaatattct
    15241 cacccagtca tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa ttgtcaccca
    15301 ggggaccgat ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg ctgcgacatt
    15361 attgcagtcc atggataacg ccaacaaagt gttcccggaa atggcgacga tgccgattgc
    15421 gctggttatt ctgattgtct gtgccattgg tgcggtgatc ggtttgatca acggtctgat
    15481 tatcgcttat ctcaacgtga cgccgttcat taccacgctc ggcacgatga tcatcgtcta
    15541 tggcatcaac tcgctctatt acgactttgt cggggcgtcg ccaatttctg gttttgacag
    15601 tggcttctct acctttgctc agggctttgt cgcgctgggg agtttccgtc tctcttacat
    15661 caccttctac gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca aaacccgctt
    15721 cggtaagaac atttttgcca ttggcggtaa cccggaagcg gcaaaagtat ctggtgtcaa
    15781 cgtcggcctg aacctgctga tgatctacgc gttgtctggc gtgttctatg cctttggcgg
    15841 gatgttagaa gccggacgta tcggctctgc caccaacaac ctcggcttta tgtatgagct
    15901 ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg tggggacggt
    15961 gattggcgtg gtgaccgggg taattatttt taccgtcatc aactatggcc tgacgtatat
    16021 cggcgtaaac ccatactggc agtacatcat caaaggggcg attattatct tcgccgtagc
    16081 gctggattca ctgaaatacg cgcgtaagaa atgatgtgaa tgtggtccgc attcggtcat
    16141 aaacgcacat aacatcatga tctgtgtggt tttgtaggcc ggataaggcg ttcacgccgc
    16201 atccggcaac aggagccaaa tgccggatgc aacgctggcg cgttttatcc ggcctacaat
    16261 aagctcaaca tactgatttg tatggttttg taggccggat aaggcgttca agccgcatcc
    16321 ggcgacagga accaaatgcc ggatgcgtct ctggttttac aacgttaccg ggtgttcttt
    16381 ctcgcctttc ttaaacttca cttccccgag ctcaatacaa cccaccgggc agacgtgacc
    16441 acaaagcaga caacccacac atttctcggt attacaatgc ggtgtgcggg ttttctcgct
    16501 ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc cacagccaac
    16561 acatttatca agattgatac ggggatagac aatataactg cggtctaaat cttccgccgg
    16621 gacaatattg ttattcgcca gacctaccat ctcctgcagc gaatcaaatc cctgatcggc
    16681 gagataatgc gacaacccgc ttgccatatc ttccactatc cgatacccgt actgcatgat
    16741 gccggtggtc acctgtaacg ttgctgcgcc gagcaataaa aactcagccg catcctccca
    16801 ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc agttctggat gggtgcgcat
    16861 ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc cggaaatact
    16921 cgattttccg ttaacgatcg gcataccgat tttctgatta agatcgatat tggtgatgga
    16981 tttaacggtg ttaatcgccg caatgccatc tgcgccgccg cgcttcgccg ccagcgccac
    17041 ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg tggaaccccg
    17101 tttcactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat cgctacccat
    17161 cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg cgccagcttc
    17221 ttgcaccagg cgcgccagct cctcccattg ctgctcattt tcccccatga tcgaagcgat
    17281 caatactttg tccgggtaat cttccttcag ccgacgcagg gcggccagat tctcttccaa
    17341 cggatgttca gcaatctgct ccatattttt gaagccgata aaaccggtat cttctttcac
    17401 cagatgatca aaacgcggcg agacttcgtt ggcgataaaa aagccgatcg ttttaaacac
    17461 cacaccgccc caacctgtgt cgtaggcttt ggcacacatc tcatagcagt tgcctaccgg
    17521 cgaagaagag aggcagaacg ggttgggaaa cttcacgccg caaaaagtaa tcgaaagatc
    17581 tttcgttaac atgagcaagc tccctctaaa tagtgatgaa tcgcctcggc ggcttctttc
    17641 ccggttttca cggcatagac cacggttttg tcaccctcaa caatatcgcc agcagcaaag
    17701 acttgcgggt cgcgggtctg gtaattttgt gttttgatgg tgttacgctg cggctctaac
    17761 tccgcaaagg catctagtct ggcatgctga ccgacggcga gaataatttt atctgccgcc
    17821 atcgtcagtt cgcccgataa ccgtacatgc ttaaacgtga ctttattgcc ttccacggct
    17881 actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggt aaactctttt
    17941 tcgcttgccg gaaactcatc taactcttca cgcgctacgc aagttaccgc ctgacagccg
    18001 agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat aattaatgcg
    18061 ctttgtggaa tgctgatatc gccttgcgcc tgtcgtgcac gttgcaagaa gtcgacggca
    18121 atctcaacgt cactatgctc aaacagcggt agcccggaac cgcttgataa ccccacggtg
    18181 accagtaccg cgcggttttc tgctttcagc tgctcaaggg tgagtgtgtt accgacttcg
    18241 ttgttgcact taatggtcac gcccattttt tcaatacggg cgatctctgc atccagcact
    18301 gattgcggta accggaattg cggaataccg ttacgcaacc agccaccggg gtgcgcttct
    18361 ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg tcacactggc ctgcaatccg
    18421 gcaggacctg cgccaataat cgcgactttg ccgagcgttt tagtaccggg ctgataaatt
    18481 tccattccgg tttgttgttc aaaatcagta acaaaacgct gtaagcggcc gatatcaatg
    18541 ggagcatcaa caccggcacg ggtacaaccg ctttggcata atttctccgt cgggcacact
    18601 ctggcacaaa cggcaccgag ggcattattt tcgcgaattg tctcggcagc gcctttaaaa
    18661 ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg gcaagcctga
    18721 ctacagggag cgtcgtgaca taataaacag cgagaggctt ctttaatcgc cagtaaagac
    18781 gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc ttccttaatg
    18841 ctataggtaa ttaaggcgag cgtttagtaa ataataatgt actgatatta aggctaagcg
    18901 tgcgggggaa tttaaactgt gaaaagattc acaaatagct gcggttagga atgtgatatc
    18961 cgcagcttat caggtgaagt aataattatt ctcaataatt aactggagag gggaatatta
    19021 acgatagaaa ataagataaa caatcatgcc gatagataaa attccgaagc cgatatatgc
    19081 caccatataa gcgaaaaaga gtccgggatt ttttgctcgc cagacataca ccggttccgg
    19141 tgcattttta acccgtttat caatcgcgcc tgcgcgccag cctttccatg cttcgcggaa
    19201 acaaaatatc gggataagga aaaaaacggc aacgacaaaa aactgctgta catccataac
    19261 tactttcctt gtttcttttg taattcaagt aactgttcgg gtgtgacggc gggatacccc
    19321 tgcgcatctt ccggtacctg gtgcatagcc ggaataggga ccagcggccc taaaaaacgc
    19381 ggttcacgtt taaaaatata aaggtcagcc agcgcaccga aacgggcggc aaattcacga
    19441 atacgtactg acagcatatc tttcggtgac ggtacggcat aacactgagc ctgaatcccc
    19501 atatgcagcg caataaataa tgctcgctca cagtggaaac gttgggtgat aataatgaaa
    19561 tcattagtat cgaaaacttt gcgtgtacgc acgatggaat ccagcgtacg aaagcctgcg
    19621 taatcgagaa caatatctga tgggtcgaca ccagcagcga ttaaatcttt gcgcatggtc
    19681 atcggctcat tataactttg caatgcgtta tcgccgctca gtaatagata atttacctta
    19741 ccgctgttat aggcattaat cgctccttga atgcggtagc gataatactg attaattacg
    19801 ccagtacgat aatattttgc tgttccgagc accacaccga cctggcggta ggggagatcc
    19861 tgcaattcgt cgtagatata aggcgcggtt ttccagctca tccagcgatc gaggccgagc
    19921 acagtcaaca gcagcaagcc gatcaggact aacaggctga ggaacacgcg ctttaacatg
    19981 caggagactc gatctaagga aaatttccat caggctactt tacccgctgg ttttgcgcaa
    20041 gaaacggcaa actgtttgcc gggaatttcg acaccaggag agggctggca agccggacaa
    20101 ggcgttcacg ccgcatccgg caccaggctt aagcgagaag cactcggtcg atactgtgac
    20161 agccgagagc tttcagcgtt gcggaggtgg catcccactg aatcaacggc gcatcggctt
    20221 tttctgccag aaccgcgcgc tggatatccg ggtaatcata acccttgaga ttcaacagaa
    20281 ttaacgctcc ctgcaacggt ggcagagtcg ggttgaatgc ggcgttttca gcgtagctgc
    20341 cactgaaaat acgaccgtct ttacattcca gcgcgacacc gcttggcgac ttactgtaag
    20401 gcatgtgcga acggtttgcc gccgcaatcg ctgcctgaga aagcgcatca cccgtcagcg
    20461 catagccgtg atcctgttcg tccatcagca gcgttttaat ctccagatct ttcggcccaa
    20521 aggcatctgg cagatagtca cgcagcgcgt gtgcctcgcg gcccggcaga tgaatacgca
    20581 gatccagacc gctgttcagt tcattcataa actgacggca gtgaccacaa ggcgtgtagt
    20641 taacggtgat ggctgcaagc gctttttcac cactcaacca ggcgtggctg atcgcgcttt
    20701 gttcggcatg aacggtttgc tgcattgtcg caccaataaa ttccatattg gcaccgaaat
    20761 accaggttcc gctcacaccg cgcgcaattg cgccaacatt aaaattcgac aatggcgtac
    20821 gcgcacaggc cgccgccagc ggaagtagtg cgaatgccag cgcgtcttcg tccagccccg
    20881 ttgcgctctt cagcgatgag acttgctccc cggtcaacaa agcggggaag tacttgtctg
    20941 ccagaatagg ttccagtgca gattgcaagt tatccgcaag ttgggcaaaa gcggtttgaa
    21001 aacgtggatg catggcgttg cctcataatc atgtaatggg ataacagttt acgaacccgt
    21061 tgtggcttta tatgtgatct gaatctcatt aattatgcaa cgcgtgaaac atacatcaaa
    21121 aatgcgcgac gcatcgcaaa ttttagccca ttactgccag aataatcggg aaaaggaacg
    21181 gtgcgatcag cgaagtaatt atcccgcata acaccagcgc tagcgaacta aatgcacctt
    21241 cctgataatc cagctcggcg cagcgcgccg taccgagggc gtgcgaggca gtccccattg
    21301 ccagaccgcg cgcagctttg gtacgaatac gcatcgcatt aagcaatgta tggccaaata
    21361 ccgcgccgag gatgccgacg aaaatcacgc aaactgcgct gattgccgga ataccgccaa
    21421 tactgccgcc aaccgccatt gcaataggcg tggtgactga tttcggcagg attgacgcgg
    21481 cgatttccgg tgaagcgccc atcaacaatg ccacggaagt ccccgtcacc atcgcaacca
    21541 cgctgccgat aaaacagatg gtgatgatcg atttccagcg cgcgcggatc tggtgtagct
    21601 gctcatataa aggataggcc agcgcgacga ccgccggttg cagcagatcg tttagcactt
    21661 cgctaccttt aaagtagctg tcgtaagaga tgccagtcag cattaaaaaa ggaatgatca
    21721 ccaccattgc taccagcaac gggttaagca agggaaactt ataccgtgcc gccagtttgc
    21781 gggcggcaaa aaagacaatc aatgtcagcg gtaatgacca ccagatattc gccatcattc
    21841 ttctgatcct ttctgaccta cgactttacg ttcaccgtgt accagttgcg aactccagct
    21901 caccaccaga aaaaccacca gcgtactgac tgcacaggaa accactaccg ggccaaactg
    21961 tgcgcggagc aaatcaaaat attgcatgac gcctacgcca atcggcacaa acaatagcgc
    22021 catatagcga atcagtacgt agcaccccgg attgacccat tttgccggaa gaatttgcaa
    22081 ggccagcagg acaaacagga tcagcatccc gatgatgctg cccggaatgg ttaccggtag
    22141 cagggaagca atgaaaatgc ctgcatacag gcaggcataa atcaggacga aagcgcgtaa
    22201 atattgccag ataatgttca gtgtcttact cataatcaac accccagatg agatggattc
    22261 atcatacaat taattcgtaa aacgtgctac cgatcacatt aagaaatcag cgcttatttt
    22321 ttcgctcccc ggcattacgc aatgcgcagc gaactcaggc atacatcaag catagagagg
    22381 gattgcagtt tgcagcaatc cctctttgca gacctttaat ccgctgacca taggcgctaa
    22441 catagcgcct catttttttg cgggtgatga tatgcgtgtg ttactggcac cgatggaggg
    22501 agtgcttgac tctctggtgc gtgaattgct gaccgaagtt aacgactacg atctgtgcat
    22561 caccgagttt gtccgcgtgg tggatcaact gctgccggta aaagtctttc atcgcatttg
    22621 ccctgagcta caaaacgcca gccggacacc atctggtacg ctggtgcgcg tgcagttgtt
    22681 aggtcagttc ccacaatggc tggcagagaa cgccgcccgt gcggtggagt taggttcctg
    22741 gggcgtggat ctcaattgcg gctgcccgtc gaaaacggtt aacggtagcg gcggcggggc
    22801 gacgttactc aaagatcctg aactcatcta ccagggtgca aaagcgatgc gtgaagctgt
    22861 accggcgcat ttgcccgtca gcgtgaaagt gcgtctgggc tgggacagcg gtgagaagaa
    22921 atttgaaatc gccgatgcgg ttcaacaggc tggcgctacg gagctggtgg tgcatgggcg
    22981 gacgaaagag cagggttacc gcgcggagca tattgactgg caggcgattg gcgatattcg
    23041 ccagcggctg aatattccgg tgattgccaa cggtgaaatc tgggactggc agagcgcgca
    23101 acaatgcatg gcgatcagcg gctgcgacgc agtgatgatt ggtcgcgggg cgctcaatat
    23161 tcccaacctg agccgggtgg taaaatataa cgaaccgcga atgccgtggc cggaggtggt
    23221 tgctttgctg caaaaatata cccgtctgga aaagcagggc gataccgggt tatatcacgt
    23281 tgcgcggatt aaacagtggt tgagttattt gcgtaaagaa tacgatgaag caacggaatt
    23341 atttcagcat gttcgggtgt tgaataattc ccctgatatt gcaagggcta ttcaggcaat
    23401 tgatatcgag aaactctaaa aaaccatagt gatgtttttt gacaatgtgt tagctcttta
    23461 tcaataggcc gcacggttgt ttggtggcgt caaaat
//
LOCUS       DAHBHC010000078        22893 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHBHC010000078 DAHBHC010000000
VERSION     DAHBHC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.078"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..524
                     /gene="escV"
                     /locus_tag="M2A35_003744"
     CDS             <1..524
                     /gene="escV"
                     /locus_tag="M2A35_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HCB7199990.1"
                     /translation="ELVKELQRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWST
                     KEKDVVILCEYVRIALRRHILGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLN
                     ISPERSEQIIGFLKNIVNPTGNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNI
                     ELKVLGTVNDFRA"
     gene            508..1848
                     /gene="escN"
                     /locus_tag="M2A35_003745"
     CDS             508..1848
                     /gene="escN"
                     /locus_tag="M2A35_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HCB7199991.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            1908..2228
                     /locus_tag="M2A35_003746"
     CDS             1908..2228
                     /locus_tag="M2A35_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HCB7199992.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            2221..2637
                     /locus_tag="M2A35_003747"
     CDS             2221..2637
                     /locus_tag="M2A35_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HCB7199993.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSITLNP
                     NNKNHIFLYHKKNYICDKLKEIFPDEPIEFSIEYEN"
     gene            2600..3517
                     /locus_tag="M2A35_003748"
     CDS             2600..3517
                     /locus_tag="M2A35_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HCB7199994.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHS
                     NTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNL
                     FIADVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCR
                     LIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            3548..4063
                     /locus_tag="M2A35_003749"
     CDS             3548..4063
                     /locus_tag="M2A35_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HCB7199995.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(4281..4640)
                     /locus_tag="M2A35_003750"
     CDS             complement(4281..4640)
                     /locus_tag="M2A35_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HCB7199996.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLGMFFLSLKEDSVLLNIAFPADKVNITEFIN
                     LMENGYLLKNEVISLLS"
     gene            4891..5502
                     /locus_tag="M2A35_003751"
     CDS             4891..5502
                     /locus_tag="M2A35_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HCB7199997.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGGDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
     gene            5949..7565
                     /gene="tir"
                     /locus_tag="M2A35_003752"
     CDS             5949..7565
                     /gene="tir"
                     /locus_tag="M2A35_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HCB7199998.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
     gene            7698..8168
                     /gene="cesT"
                     /locus_tag="M2A35_003753"
     CDS             7698..8168
                     /gene="cesT"
                     /locus_tag="M2A35_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HCB7199999.1"
                     /translation="MSSRSELLLDRFAEKIGIGSISFNENRLCSFAIDEIYYISLSDA
                     NDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLD
                     DATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            8229..11048
                     /gene="eae"
                     /locus_tag="M2A35_003754"
     CDS             8229..11048
                     /gene="eae"
                     /locus_tag="M2A35_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HCB7200000.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGEIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSESLTAWIKQSSSEQSSGVSST
                     YDLVTKNPLINVGVNNKNAFSVCVK"
     gene            complement(11263..12483)
                     /gene="escD"
                     /locus_tag="M2A35_003755"
     CDS             complement(11263..12483)
                     /gene="escD"
                     /locus_tag="M2A35_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HCB7200001.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLRSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFYYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            12626..13681
                     /gene="sepL"
                     /locus_tag="M2A35_003756"
     CDS             12626..13681
                     /gene="sepL"
                     /locus_tag="M2A35_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HCB7200002.1"
                     /translation="MANGIEFNQNTASVFNSNSLDFELESQQLTQKNSSNSYSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLFDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVI
                     LNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRD
                     NTLLMIGKVIDYKEDVI"
     gene            13739..14317
                     /gene="espA"
                     /locus_tag="M2A35_003757"
     CDS             13739..14317
                     /gene="espA"
                     /locus_tag="M2A35_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HCB7200003.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQAVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            14330..15472
                     /gene="sctE"
                     /locus_tag="M2A35_003758"
     CDS             14330..15472
                     /gene="sctE"
                     /locus_tag="M2A35_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HCB7200004.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            15493..16437
                     /gene="espB"
                     /locus_tag="M2A35_003759"
     CDS             15493..16437
                     /gene="espB"
                     /locus_tag="M2A35_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HCB7200005.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            16444..16851
                     /gene="cesD2"
                     /locus_tag="M2A35_003760"
     CDS             16444..16851
                     /gene="cesD2"
                     /locus_tag="M2A35_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HCB7200006.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            16888..17109
                     /gene="escF"
                     /locus_tag="M2A35_003761"
     CDS             16888..17109
                     /gene="escF"
                     /locus_tag="M2A35_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HCB7200007.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            17115..17393
                     /locus_tag="M2A35_003762"
     CDS             17115..17393
                     /locus_tag="M2A35_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HCB7200008.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCCLVHENNRSWTLMF"
     gene            17609..18232
                     /gene="espF"
                     /locus_tag="M2A35_003763"
     CDS             17609..18232
                     /gene="espF"
                     /locus_tag="M2A35_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HCB7200009.1"
                     /translation="MLNGISQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSIPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSL
                     DTSGLKPSRPAPPPPTSGQASEASRPLPPIAQALKDHLAAYELSKKA"
     gene            complement(18547..19131)
                     /locus_tag="M2A35_003764"
     CDS             complement(18547..19131)
                     /locus_tag="M2A35_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HCB7200010.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDERTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKLIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
     gene            19329..19967
                     /locus_tag="M2A35_003765"
     CDS             19329..19967
                     /locus_tag="M2A35_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HCB7200011.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     VSFMDAFSVEPIQGSLLDRLGGRCRRLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLTDQRFWIISGQDVRF"
     gene            complement(20340..20684)
                     /locus_tag="M2A35_003766"
     CDS             complement(20340..20684)
                     /locus_tag="M2A35_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCB7200012.1"
                     /translation="MKSLTTETVLDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     VGGSSLTFRFSQLITDRLFILTGSPVRPVSLLQFSIVIAVISSGNEARTPCHNFLYAD
                     NHFPVHLPVAGT"
     gene            complement(20701..21189)
                     /locus_tag="M2A35_003767"
     CDS             complement(20701..21189)
                     /locus_tag="M2A35_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200013.1"
                     /translation="MKLALTLEADSINAQTLNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAEYLPGLPTF
                     DW"
     gene            complement(21186..21562)
                     /gene="cbtA"
                     /locus_tag="M2A35_003768"
                     /pseudo
     CDS             complement(21186..21562)
                     /gene="cbtA"
                     /locus_tag="M2A35_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            complement(21651..22019)
                     /gene="cbtA"
                     /locus_tag="M2A35_003769"
     CDS             complement(21651..22019)
                     /gene="cbtA"
                     /locus_tag="M2A35_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCB7200014.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(22093..22314)
                     /locus_tag="M2A35_003770"
     CDS             complement(22093..22314)
                     /locus_tag="M2A35_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCB7200015.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(22383..22859)
                     /locus_tag="M2A35_003771"
     CDS             complement(22383..22859)
                     /locus_tag="M2A35_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCB7200016.1"
                     /translation="MQQLSFLPVAMTPGERSLIQRALKTLNRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADQLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         6626 a         4340 c         5248 g         6679 t
ORIGIN      
        1 acgaacttgt gaaagagctt cagcgtcagc ttggtctgag caagattgtg gatattcttc
       61 agcgtctcgt agaggaaaat atctcaatta gagatctacg aactattttt gaaacgctta
      121 ttttttggtc cacaaaagaa aaagatgtgg ttatcttgtg cgaatacgtt cgtatcgccc
      181 tccgtcggca tattttaggt cgctatagcg ttcgcggtac acttctgaat gtctggctta
      241 ttggctctga tattgaaaat gagttacgag agtcgatcag acaaacgtca tcaggttcgt
      301 atttgaatat ctcaccggag cgaagtgagc agattattgg cttcttaaaa aatattgtga
      361 atccaacggg gaatggcgtc attctgaccg ctttagatat caggcgctat gtgaagaaaa
      421 tgattgaagg ttcgttcccg tcagtccccg tgctctcttt tcaggaagtt gggaataata
      481 tcgaacttaa agtattagga acggtaaatg attttagagc atgattctgt attggaaaga
      541 tacccacgta tccagaaagt gctcaatagc actgtgccga cattatcatt aaattcgtct
      601 accagatatg aaggcaagat taccaatatc ggcgggacga ttatcaaggc gcgtttacca
      661 aaagcgcgta ttggcgcctt ttacaagata gaacccagtc aacgtttagc tgaggtgatc
      721 gctattgatg aagatgaggt tttcctgctg ccttttgagc atatttccgg catgtattgt
      781 ggccagtggc taagttatca gggggaagag ttcaagattc gtgttgggga cgaactcctt
      841 gggcgactgg ttgatggtat aggcagacca atggggagca atattactgc accttatctt
      901 ccgtttgaac gcagtttgta tgctgaaccc cccgatcctt tattaaggca ggttattgat
      961 cagccattta cgcttggcgt cagggcaata gatggtttac ttacttgcgg tattgggcaa
     1021 cgcatcggta ttttcgcggg gtccggcgtt ggtaaaagta cgcttttggg aatgatttgt
     1081 aatggcgcat ccgcggatat tattgtcctt gcccttattg gcgagcgtgg tcgcgaagta
     1141 aatgaattcc tcgcgctttt acctcaatcc acgctttcta aatgtgtact ggtcgtcaca
     1201 acgtcagacc gtcccgcgct ggaaagaatg aaggccgcat ttacggcgac aaccattgca
     1261 gagttcttcc gggatcaggg caaaaatgta ttattaatga tggattccgt aactcgttat
     1321 gcccgagccg cgcgtgatgt tgggcttgca tcgggagagc ctgatgtaag ggggggattc
     1381 cctccgagtg ttttttcctc gttacccaaa ttattagagc gtgcgggacc tgcgccaaaa
     1441 ggttcaataa ctgcgattta tacggtgctg ttagaaagcg ataatgtgaa tgatcccatc
     1501 ggtgatgaag ttcgctctat tcttgatgga catattgtgc ttacccgaga acttgcagag
     1561 gaaaaccatt tccctgcaat tgatattggt ttaagcgcca gtcgtgttat gcataacgtt
     1621 gttaaatcgg aacatttacg tgcagcggct gaatgcaaaa agcttattgc aacctataag
     1681 aatattgaac tgcttattcg tattggtgag tacacgatgg ggcaagatcc tgaagcggat
     1741 aaggcaataa aaaacaggaa agcaattcag agctttatac aacaaagcac caaagatatc
     1801 agtagttacg aaaaaacgat tgaaagccta ttcaaagtgg ttgcctgaat atgttggaca
     1861 gaattttatc tattcgtaaa agcagagcga atcgattgag agaatcaatg gctaagatta
     1921 actctcagat aaaagaggtg gatggtaaac tcgatgactg cgagcaagcc attaaagagt
     1981 cgattgcctc taagcaggcg tattgtgcat cgctggtgaa tctggataaa gtgtcgttgt
     2041 ataaatacca gattaaaaac aatgcatttg atgagcaaaa gcagcgatta tatgagaaaa
     2101 aatcctcact ttctaaagaa aagcgttcat tgctcgattc acaaaaacga acaaaagagg
     2161 atctacaaca tgttaataaa tcaatagaga agttatcttt tgccataaag gaacattact
     2221 ttgactagca tttctctaaa aagaaatttg atagagcctg tttcttttca gccgtcagat
     2281 gattggccgc agggagaaga aagttttaat gatattttta atcgcataaa aaaaagaaca
     2341 cctgacactt cgttcatacg aatggcttct ttatggaaac gattgttcta cgctcctgga
     2401 cagaggcgac gctactttga acaaggagag cgttcattta gtattatttg tggaaagtta
     2461 cgcggaattg ttttaacaat aaaatgtagt aatggaacca tttatctgtc tattacactg
     2521 aaccccaata acaaaaatca tattttttta taccataaaa agaattatat ttgtgataag
     2581 ttaaaagaaa tctttcctga tgaacctatt gaattctcaa ttgaatatga aaattaatga
     2641 tttttacttg ccattgttgt cagtcatagg aaccggtcgc ctctatatta cgcctgaagg
     2701 gcatgcttgc catgcatatt ttcgcgaagt cagcggtaat ggtatccgct tcacgttaac
     2761 atgctcaggc tatgaggggc gtttttggat atcagaagaa cagttcattc agtggtgcca
     2821 ggagttattc ccgtatagcg aaagccggct gataccggaa gatatgataa aactcatgat
     2881 cttatgggta atgcagactg ctttaccaga aggtgatgtg tctgttgatg atgttcaatt
     2941 tacgatgcta aataaagatg tttatccggt aatagaaaat aataatggag aaaacagact
     3001 taatgtaatt atactcgaaa taacggtaca gagtttacaa tatctaatta atgaaaattg
     3061 gcaatttgtt ccacattcta ataccatttt ctttgatgga tatattgctc ctgggtggac
     3121 tgattacccg gttacagagc tgactgttgg tgatagttta aggctctatc atgttgatga
     3181 cagcaaagaa agagagtgtt ggcttgttat taataaccct ttagcaaccg taaaactttg
     3241 tgataataat ttatttattg cggatgtcca ggctgcagat ttgttatgta ctatcagcaa
     3301 tgaggctgta atggatcgca tttattgtgt tattggaaca attcatgttg atatacatat
     3361 gttgcgcaat gccaaaaaag atgacattat cgattcaact ggatatcatc tttttggtgg
     3421 gtgccggtta atacgcaata atacgactat agcatacgga agcattgtaa aaatcaacga
     3481 agatttttat tttactgtta gtttagtatg tgattaatca ggaataagtt atatagggag
     3541 atgtattatg tcgtcatcgt tatcaggaat aatactcaat acttcgctga caagtcacca
     3601 tatgtcatgg ggcaagttta gcagaactct tcatgtattg aatgaagata tcgtagatga
     3661 aattcagctt aaagcccgaa cacaacaacg ccaccatcga gtatattcag aaatggcgga
     3721 ttactccgtt gtctcctttc ggggcaaaga atattctgtt cgttttatta aagattacaa
     3781 aacagataat tatgtgtaca aagtacaaaa gatgactcag gaaagtgggt gtctctcaag
     3841 attatttagc tttctttctg gcggtgtgac taaggctttg gaaagtaaac taaatgagag
     3901 gcatatcact ccactctctc caacctggtt tcctcgaact ccactggagg gggtcttaac
     3961 ggaaagagga ttatcttctt tgttacgtcg cgtacagtct actgaaaacc ttgattatag
     4021 taatgctatg atatcaaact ctttctctaa ctccgtagtg tgattatcat ttgagctttc
     4081 ctcctggttt aactccagaa taattgtgat tctggagttt ttctgtttgt tttatgtcga
     4141 taatacattg ttttaggaaa atatgggatg aatattcaga gtgagtttgg ctggcagaag
     4201 tcagagattc tgtgatgagg aggggattga gctgaatgga tgaattcctt ccagctcata
     4261 atttatcagg ataaaacatc ttacgacaat aaactaatta cttcattctt taataaataa
     4321 ccgttttcca ttaaattgat aaactctgta atgttaacct tatctgcagg gaatgcaata
     4381 ttcaaaagta cgctatcttc ttttaatgat agaaaaaaca taccgagtag atctcgattg
     4441 ctatttattt ctagtatttt atcaattttt gaagattcat tattgaataa ataacaataa
     4501 aaaaacagga cctcagattg aggtgcacac tccagtatcc attcatgacc atcgtgatca
     4561 tggaaagata gggcgttagt ttgttcttca agagttattc cgaacgtatg tgcaagatct
     4621 gtgcgaaatt gttcattcat attgtagtcc tgtcattctt tcaatgaggg tatgcttaaa
     4681 cgtaattaac tcgtgagctc tggcacaaat aataagacga aaatataaga tgcaagtaat
     4741 agtgttgcat atttatgata gcatgtgata aatgtagtgc aactacttgt agatagtgcc
     4801 ggaggaaaat ttaaatataa aataattatt gtttttaatg aaaataaaaa acattttgtt
     4861 atcgttatat aatatataaa ggtttattgt atgtttagtc caatgacaat ggtaggtaga
     4921 tcgttagctc aggcggctac acaaactctt agacctgcgg tcaccagagc tgcgacacaa
     4981 gcgggggtgg gagccagtgg catgaagttc gtgcccatgc agtcgaactt tatgattaat
     5041 catggcaaac tgactaacca gctacttcag gccgtggcca aacaaacccg tagcggtgat
     5101 actcagcaat ggttcaaaca ggagcaaatt acttatatat ccagaacagt aaacagagct
     5161 attgatgact attgcatgag taataattcg gtaattggta aggaaaccaa aagccacatt
     5221 ttcaaggctg ttgaaagcgc gataaagcag cctctcgata tgaattgtgc acaaacgtcg
     5281 attggccatt tcttacagtc caacaaatac tttaataaaa aagttgatga gcagtgtggc
     5341 aaaggggggg atccaattac acgctttaac acccagacca ggatgataga acaagtctca
     5401 caagaaatct ttgaacagaa atttggtggt ttgaaagtta gtgatatcaa agctctgact
     5461 cagagcacga ttgcacagca tgtacaggat actcggttgt aggaataacc tgttatatat
     5521 gcgtgttggg cataagttaa gcttcctggt gtatagcgta gtctttgctg atgtaagaaa
     5581 aggctatata cggaaacttg atatactata aacttcatta tttattatat tgaaaaagtt
     5641 atttaattga tttgttttgt aattttaatg ctttaattgg ataagcataa aatatagttt
     5701 tatttatagt gtgttttatt aaagtttcat gtggtattta tttttatatc gcatgattat
     5761 tttcatttaa ttacactgat gtgtattttt ctgcattatt ttcttgcatc aaaaatcaga
     5821 ctgtgattta tttggtcgat gttatgttca gccgtatatc gactacgtac tgtataccat
     5881 tacattatta tgtaatagca atataaagga ttattatcta tttacataaa ataaaaagga
     5941 gatattttat gcctattggt aatcttggcc acaatcccaa tgtgagagct ttaattccac
     6001 ctgcaccgcc attaccttca caaaccgacg gtgcaggagg tgcccgtaat cagctcatta
     6061 actcaaatgg cccgatgggg tctcgtttgc tatttacgcc tataaggaat tctgttgctg
     6121 atgctgctga ttctcgtgcc agtgatattc ccggacttcc tacaaatcca ctgcgctttg
     6181 ctgcgtccga ggtatctttg catggtgcac ttgaagttct tcatgataaa ggggggcttg
     6241 atactcttaa ctctgctatt ggatcttcgt tattccgtgt tgaaactcgg gatgatggca
     6301 gccatgttgc tatcgggcaa aaaaatggcc tcgagaccac tgttgtttta agtgagcaag
     6361 agttttctag cttacagtcc cttgatcctg aaggtaaaaa caaatttgta tttactggag
     6421 gccgcggtgg cgcagggcat gctatggtca cggttgcttc agatatcgcc gaagcccgtc
     6481 agaggataat agataaatta gaaccaaagg atacaaagga gacgaaggag ccaggggatc
     6541 caaatagtgg cgagggaaaa atcattgaaa ttcatacctc aacctcaact tctagcctcc
     6601 gtgcagatcc taaactttgg ttgtcattgg ggactattgc tgcaggtctg atagggatgg
     6661 ctgcgacggg gattgcacag gctgttgcgt tgactccaga gccggatgac ccaatcacta
     6721 ccgaccctga tgctgcagca aacacagctg aagcagcggc aaaagatcag ttaacgaaag
     6781 aagcattcca gaacccagat aaccagaaag ttaatatcga tgagaacgga aatgcaattc
     6841 cgtccgggga actaaaagat gatgttgttg cgcaaatagc agaacaagct aaagcggcgg
     6901 gtgaacaggc cagacaggaa gctattgaaa gtaattctca ggcgcagcaa aaatatgatg
     6961 aacagcatgc taaacgcgaa caggaaatgt ctctttcatc gggggttggc tacggtatta
     7021 gtggtgcgct gattcttggc gggggaattg gtgccggtgt tactgctgct cttcatcgga
     7081 aaaaccaacc ggcagaacaa acaatcacta cacgtacggt agtcgataat cagcctacga
     7141 ataacgcatc tgcgcagggc aatactgaca caagtgggcc agaagagtcc ccggcgagca
     7201 gacgtaattc gaatgccagc ctcgcatcga acgggtctga cacctccagc acgggcacgg
     7261 tagagaatcc gtatgctgac gttggaatgc ccagaaatga ttcactggct cgcatttcag
     7321 aggaacctat ttatgatgag gtcgctgcag atcctaatta tagcgtcatt caacattttt
     7381 cagggaacag cccagttacc ggaaggttag tgggaacccc agggcaaggt atccaaagta
     7441 cttatgcgct tctggcaagc agcggcggat tgcgtttagg tatgggagga ttaacggggg
     7501 ggggcgagag cgcagtaagt actgccaatg ccgcaccaac gccgggaccc gcacgtttcg
     7561 tttaaatata tccgtgagta tttagttgag gttgggaggg ggggagtttt actaatatta
     7621 atgtttcaga gaataaagtt gcagcatggg taactcttga acttctgtaa ttataaattc
     7681 aattaagaga aattataatg tcatcaagat ctgagctttt attagataga tttgcagaaa
     7741 aaattggtat tggatctatt tcatttaatg aaaatagatt gtgttctttt gctattgatg
     7801 agatttatta tatttcgtta tctgatgcta atgacgaata tatgatgatc tatggcgtct
     7861 gtgggaaatt cccgacagat aatcctaact tcgctcttga gattttgaat gcaaacttat
     7921 ggtttgcaga gaatggtgga ccatacctgt gttatgaatc aggagcacaa tcgctgttgt
     7981 tagcgttacg ttttccgctc gatgatgcta cacctgaaaa acttgagaat gaaatagaag
     8041 tcgtcgttaa gtcaatggaa aacctgtatt tggtattaca taatcaggga ataacattag
     8101 aaaacgaaca tatgaaaata gaggaaatta gttcaagcga taataaacat tattacgccg
     8161 gaagataaaa gattatttat taatataatt tactattcat tctaactcat tgtggtggag
     8221 cccataacat gattactcat ggtttttatg cccggacccg gcacaagcat aagctaaaaa
     8281 aaacatttat tatgcttagt gctggtttag gattgttttt ttatgttaac cagaattcat
     8341 ttgcaaatgg tgaaaattat tttaaattga gttcagattc aaaactgtta actcaaaatg
     8401 ccgctcagga tcgccttttt tatacgttaa aaacaggtga aactgttgcc aatatttcta
     8461 aatcacaggg tatcagttta tcggtaattt ggtcactgaa taaacattta tacagttccg
     8521 aaagcgaaat gatgaaggct ggacctggtc agcagatcat tttgccactc aaaaaactgt
     8581 ctgttgaata tagtgcctta cctgtcttag gttcggcacc tgttgttgct gcaggtggtg
     8641 tcgctggtca tacgaataaa atgactaaaa tgtccccgga cgcgactaaa agcaacacga
     8701 ccgatgacaa ggctctaaat tatgcggcac aacaggccgc gagccttggt agccagctcc
     8761 agtcgcgctc actgaacggc gattacgcga aagataccgc tcttggtatg gccagcagcc
     8821 aggcttcgtc acagttgcag gcctggttac aacattatgg aacggcagag gttaatctgc
     8881 agagtggtaa taactttgac ggtagttcac tggacttctt attaccgttc tatgattccg
     8941 aaaacatgct ggcatttggt caggtcggtg cgcgttacat tgactcccgc tttacggcaa
     9001 atttaggtgc tggccagcgt tttttccttc ctgaaaatat gttgggctat aacgtcttca
     9061 ttgatcagga tttttctggt gataataccc gtttaggtat tggtggcgaa tactggcgag
     9121 actatttcaa aagtagcgtt aacggctatt tccgcatgag cggctggcat gagtcataca
     9181 ataagaaaga ctatgatgag cgcccggcaa atggttttga tatccgcttt aatggctatt
     9241 taccatcata tccggcatta ggcgccaaac tgatgtacga acagtattat ggtgataatg
     9301 ttgctttgtt taattccgat aagttgcagt cgaatcctgg cgcggcgacc gttggtgtaa
     9361 actacactcc gattcctctg gtgacgatgg ggatcgatta ccgtcatggt acgggtaatg
     9421 aaaatgatct cctttactca atgcagttcc gttatcagtt tgataaaccg tggtctcagc
     9481 aaatcgagcc acagtatgtt aacgagttaa gaacattatc gggcagccgt tacgatctgg
     9541 ttcagcgtaa taacaatatt attctggagt acaaaaagca ggatattctt tctctgaata
     9601 ttccgcatga tattaatggt actgaacaca gtacgcagaa gattcaattg atcgttaaga
     9661 gcaaatacgg tctggatcgt atcgtctggg atgatagcgc attacgcagt cagggcggtg
     9721 agattcagca tggcggaagc caaagcgcac aagactacca ggctattttg cctgcttatg
     9781 tgcaaggcgg cagcaatatt tataaagtga ccgctcgcgc ctatgaccga aatggtaata
     9841 gttctaataa tgtacagctc actattaccg ttttaccgaa tgggcaggtt gtggaccagg
     9901 ttggggtaac ggactttacg gctgataaaa catcggctaa agcggatggc atagaagcta
     9961 ttacctatac cgcgacggtt aaaaagaatg gtgtagctca ggctaatgtc cctgtaacat
    10021 ttagtattgt atccgggact gcaactcttg gggcaaatag tgccagaacg gatggtaacg
    10081 gtaaggcgac cgtaacgctg aagtcggcta cgccaggaca ggtcgtcgtg tctgctaaaa
    10141 ccgcggagat gacttcgcca cttaatgcca gcgcggttat atttgttgat caaaccaagg
    10201 ccagtattac tgagattaag gctgataaaa caacagcgaa ggcagatggt tctgatgcga
    10261 ttacctatac tgtcagagtg atgaaggagg gggcacccgt agtagatcag aaagtgacct
    10321 tttctaagga ttttgggacc ctgaataaga ctgaagcaac aaccgatcag aatggttatg
    10381 ctactgtaaa attatcatcc aatactcctg gcaaggccat tgttagtgca aaagtgagtg
    10441 gagtaggtac agaagttaag gctactaccg ttgagttttt tgccccgttg agtattgatg
    10501 gtgataaagt gaccgtaatt ggtactggta tcacgggggc tctgccaaag aactggttac
    10561 agtatggtca ggttaagcta caggcaacag ggggcaatgg aaaatacaca tggaaatcca
    10621 gtaatactaa aattgcttct gttgataact cgggagtgat aaccttaaat gaaaaaggga
    10681 gtgccacaat tactgtagta tctggtgata atcagagtgc gacatacaca attaatgcac
    10741 cgggtagtat tgtaattgct gtggataaaa atactcgagt tacgtatttt gatgccgaaa
    10801 acaaatgtaa gacaaatagc gcaaatttag cacagtcaaa agaactattg gccaatatct
    10861 attcaacatg gggtgctgca aataaatatc cttactattc tggttctgaa tcattgactg
    10921 cttggattaa acaatcctct tctgaacagt catcaggtgt atcaagcaca tatgatttgg
    10981 ttacgaagaa cccgttgatc aatgttggag taaacaataa gaatgctttt tctgtttgtg
    11041 taaaataatt taattacatc tcaaatcccg gaaaataaaa ttcattttcc gggatttttt
    11101 attgtaccta cttgaatctt ttaataaacg tttcatgtta aaatcaatgt ctcatttcgg
    11161 aataagtttt tcgtgtatct tgtttgatag agaccttgtt taccatatct acaacatctg
    11221 cacaataaaa aaccctccga agagggggaa gataataata agttaataag acaacggaat
    11281 gtgtatatat tcgtctgggc gggagatgtt gataccatct ttaggatcaa tcgcgtctat
    11341 gacatagcct cctttcagaa tgctaccaat tcccaggctt gtaccgttat caagagcaat
    11401 tgttggattc ttcatcgtgc cgctgattct gagtattttt ccggggaaaa tatctgctga
    11461 aatgtacggc tgaatgttct gaaatagtat cttgacgtta ctatttttgt tcatcgcatt
    11521 tataatggca aggatcttac tctcgttctg ttgagaaacc tcccccgcaa caatgatgac
    11581 atcattcttt ttgaaaatat tgacatagtt tattaattta ttctttttga actctgatgc
    11641 cagttcattg attttattgt tactgttgtt atgtatttcc catccagcaa cccctggcgt
    11701 attcagtaac agattttcaa cttcgctcca ttttggtgaa gggggaatgt atcccgataa
    11761 caggaagaat ccaggcttat taccttttgt aatgatgata tccttataac cgtattccgt
    11821 taaaatatca ttaatcgcgg agatgatttg gttattgcaa atgatattgt tgtaataaaa
    11881 aatatttttt tctttcaggt aataaaaaaa tggagttaaa tcagtggttg attcgcaacg
    11941 cccataaagc gcgacatttt tcccatgcca taccagagtg attgcatgaa gtttgttttc
    12001 ttttagctgc tggtatacat cagctctggt aaacgctggg ggttcagctt ttggggaacg
    12061 caaaacgtaa ctgccgatgg cgccagacaa cgcaatcgca gcaccaatca cgctagccag
    12121 gataagacgc tttttacgtc gatttttggc agagaacctt tctggcacat cagacaatgg
    12181 caaggtgtgg tcaatatcac caatgatgaa gcaacatccc gctatatcga tgacctttcc
    12241 gacaggtaat gactgatctg cttcataggg ggtgggctgt ccatcaatcc aaaattcaac
    12301 cggagataaa aggaaaacac cttcctcacg tatatcaagt aaaaactgat tgaggccttg
    12361 ttcaagggga aaataaacaa tatcgttatc ttctgtgcct atcgttaggt ttcccatcgg
    12421 aagttgtaat tcccgattac gcatcgcacc attgagaagt tttattttat atgaggataa
    12481 catttttaaa tttttttcat cctggtggtt gaacaatgag aaaaaatatg gtgaacttac
    12541 atcaactaag aatagagtag aaaggaagct gttgtacagt actatttaat ggaatattca
    12601 taattaatga ttacgtgagt ttccaatggc taatggtatt gaatttaatc aaaacaccgc
    12661 atctgttttt aattctaatt cattagattt tgaattggaa tctcaacaat taacgcaaaa
    12721 aaattcttct aatagttatt ctccattatt aaatttacaa aatgaattag cgatgattac
    12781 tagttcatcg ctttctgaaa cgattgaagg gctaagcctg ggatatcgca aaggcagtgc
    12841 aaggaaagaa gaggaaggta cgacaacgga gaagctgctt aatgatatgc aagagctgct
    12901 tactctgacc gatagcgata aattaaaaga actatcatta aagaacagtg ggctgctgga
    12961 acagcatgat cctaatttgg ctatgtttgg taatatgcca aaaggggaga ttgttgcact
    13021 tatatcttct ttattacaat ctaagtttgt taagattgaa ttaaaaaaga aatacgccaa
    13081 attattatta gatttattag gcgaagatga ttgggagttg gctctgcttt cctggttagg
    13141 ggtgggtgag ttaaatcagg aaggtatcca gaagatcaaa aagctttatg aaaaggccaa
    13201 ggatgaggat tctgaaaagg gagcctcttt atttgactgg tttatggaga ttaaggatct
    13261 tccagagcgt gagaagcact taaaggttat tattagggcg ctatcgttcg atctgtctta
    13321 tatgtcttct tttgaagata aggtgaaaac atcttcaatt attagcgatt tatgccgggt
    13381 aatcattttt ttatcgcttg ataactatac agatattatt gcaatctcta ttaataaaga
    13441 taaagatgtc attttaaatg aagtattgtc tattattgaa catgtatggt taacagaaga
    13501 ctggctcctg gaaagccctt ctcgggtatc gattgtcgaa gataaacatg tttattattt
    13561 ccacctattg aaagactttt ttgcatcatt acctgacgct tgttttattg atagggaaca
    13621 gagagataat acattattaa tgattggtaa agtaattgat tataaggagg atgttatttg
    13681 atattggttt tttaatcgtt tttggtcttg ctaagaaaga ttattaagag gtatatacat
    13741 ggatacatca actgcaacat cagttgctag tgcgaacgcg agtacttcga catcgacagt
    13801 ctatgactta ggcagtatgt cgaaagacga agtagttcag ctatttaata aagtcggtgt
    13861 ttttcaggct gcgcttctca tgtttgccta tatgtatcag gcacaaagcg atctgtcgat
    13921 tgcaaagttt gctgatatga atgaggcatc taaggagtca accacagccc aaaaaatggc
    13981 taatcttgtg gatgctaaaa ttgctgatgt tcagagtagt tctgacaaga ataagaaagc
    14041 caaacttcct caagaagtga ttgactatat aaatgatcct cgcaatgaca ttacagtaag
    14101 tggtattagc gatctaaatg ctgaattagg cgctggtgat ttgcaagcgg tgaaggccgc
    14161 tatttcggcc aaatcgaata acttgaccac ggtagtgaat aatagccagc ttgaaataca
    14221 gcaaatgtca aatacgttaa acctattaac gagtgcacgt tctgatattc agtcactgca
    14281 atacagaact atttcagcaa tatcccttgg taaataaccg gagataacta tgcttaatgt
    14341 aaatagcgat atccagtcta tgaggtctgg ggccagcgct gctacggcta cttcaggtat
    14401 taatcagcct gaagtgactt ccgcgctgga tttacaactg gttaaatcca cggccccatc
    14461 agcaagttgg acagaaagta ccgctttacc aacgccaccg gcaggccact cattagtgac
    14521 gccctctgtt gctgaggatg tcctgagtaa actgtttggt ggtattagtg gtgaagttag
    14581 gagtcgaaca gagggaaccg aaccacagcg cagcactcag aatgcaagct ctggctatcc
    14641 ttatctttct caggtgaata acgttgaccc tcaggccatg atgatgatgg tcaccctgtt
    14701 atccctggat gcttcagcgc agaaagttgc gagtatgaaa aactccaacg agatctacac
    14761 ggatgggcaa aataaggcgc tggataataa aacgctggag tttaaaaagc agctcgaaga
    14821 acaacagaaa gccgaagaga aagcacaaaa aagtaaaatt cttggtcagg tctttggttg
    14881 gttgggcgtc gcggtaactg ctattgcagc tatttttaac ccggcactct gggctgttgt
    14941 tgccgttagc gcaacagcaa tggcactgca aactgcagtt gatgtaatgg gcgataaggc
    15001 cccgcaggcg ttaaagacag cagcgcaagt ctttggcgga gtatctattg ctgcaagtat
    15061 tctgacagcc ggcattggcg gggtgtcttc acttatgtct aaagttggcg atgtcgctaa
    15121 caaagttggt tcaaacatcg tgaaaacagt tactaaactg gccgatgtat ttgttgagaa
    15181 cgttgtttca aaagttgcgg caacggcaaa tggatttacg acctctgcac gttctattgg
    15241 gacaactgtg ctgaataatg attctgcgca atacagcata ttggctcagt tatctgctta
    15301 cgcggtacaa aacttaactc gacagagtga aaacctgggt gagagtgcga aggttgagct
    15361 ggataaagct gcagccgagt tgcgaaacca ggcaagctat ttacaaaatg cttctcagtt
    15421 aatatccgac tcagcacgag taaatagccg tattgttagt ggtcgagttt aattattaaa
    15481 gagaatttaa ttatgaatac tattgattat actaatcaag taatgacggt taattctgtt
    15541 tcggagaata ctaccggctc taatgcaatt accgcatctg ctattaattc atctttgctt
    15601 accgatggta aggtcgatgt ttctaaactg atgctggaaa ttcaaaaact cctgggcaag
    15661 atggtgcgta tattgcagga ttaccaacag caacagttgt cgcagagcta tcagatccaa
    15721 ctggccgttt ttgagagcca gaataaagcc attgatgaaa aaaaggccgc tgcaacagcc
    15781 gctctggttg gtggggctat ttcatcagta ttggggatct taggctcttt tgcagcaatt
    15841 aacagtgcta cgaaaggcgc gagtgatatt gctcaaaaaa ccgcctctac atcttctaag
    15901 gctattgatg cggcttctga tactgcgact aaaacgttga ctaaggcaac ggaaagcgtt
    15961 gctgatgctg ttgaagatgc atccagcgtg atgcagcaag cgatgactac agcaacgaga
    16021 gcggccagcc gtacatccga cgttgctgat gacattgccg attctgctca gagagcttct
    16081 cagctggctg aaaacgctgc agatgccgct cagaaggcaa gtcgggcaag ccgctttatg
    16141 gctgcagtag ataagattac tggctctaca ccatttattg ccgttaccag tcttgccgaa
    16201 ggcacgaaga cattgccaac aacggtatct gaatcagtca aatctaacca tgagattagc
    16261 gaacagcgtt ataagtctgt ggagaacttc cagcagggta atttggatct gtataagcaa
    16321 gaagttcgca gagcgcagga tgatatcgct agccgtctgc gtgatatgac aacagccgct
    16381 cgcgatctca ctgatcttca gaatcgtatg ggtcaatcgg ttcgcttagc tgggtaattg
    16441 atcatggtcg atacgtttaa tgatgaagtg cttaatcact atcttgaaca aaaagggtac
    16501 acaatacaga aggagtttct ttgtggcagt gcctttttta tcggatggcg gattgagacc
    16561 tcttttttct cattagcgta cagacttgat gaacaagaac tgattttgtg ctcttttgaa
    16621 gcacgtaacc aaacagggct taacggccct gttttatcac tgactcgctt gctcgaagag
    16681 ttgtaccacc atttttcggg tattaagaaa atcagtgcga tgaaatctaa gattggttca
    16741 gattcagaac gtcaaaagcg cgaagagttg tttaattact tcatcagaaa gggcgctgtg
    16801 cagcaagaaa cagaagacgg aatttggttc gtaatgaatg taaatagtta gaaattttta
    16861 tttctattaa ctattgagga aaatttaatg aatttatctg aaattactca acaaatgggt
    16921 gaagtaggta aaacgctgag tgattctgtg ccagagttac ttaatagcac cgatttggtt
    16981 aatgaccctg aaaaaatgtt agagctgcag ttcgcggttc agcaatattc tgcttatgtt
    17041 aacgtagaaa gtggaatgct gaaaacgata aaagatttgg tctcaaccat ttccaaccgt
    17101 agcttttaat ttttatggtt aatgatgttt ctgccaacaa gatactggtg tgggcggctg
    17161 tagctgcggc aaatcataag cttcccaaat atgcagaatc tatcctgaat gtattgccgc
    17221 aaattatacc cgataaaaaa gatatcgcac atttagaatt tattatttta tatggattaa
    17281 atagaaaaaa tgatgcggta aaggctctgg aggactttat ggatgatgag acaagccagt
    17341 tgttatgctg tctggtccac gagaataata gaagctggac actaatgttt taatctcata
    17401 ttgagtttaa acaattgata aatttcattt tggtatgcta taaattttaa cagagatatt
    17461 tataatacct taatgaaaaa tatagctgta cgaaagtttc tatgtttcca tgtgtgtttg
    17521 atttaatagt ttatagtcgt taactatttt tgattttaat ataaattaat aattattcct
    17581 gttaattaga tataaaaagg catgaattat gcttaatgga attagtcaag ctgtttctac
    17641 actaggacgg catattacta gtgcggcaag ccgggtaagc tctgcggggt tatctggctt
    17701 ttcagtgtcc cctcaggctg ttcgtctgaa tccgattagg gctcaatctc ctttttctcc
    17761 cggtacgtcg aatatcaatg cgagaacgac ttttaatgtg agtatcccgg cgacttcatt
    17821 tactccctct cgtccggccc caccgccgcc gacaagtgga caggcttccg gggcatcccg
    17881 ccctttaccg cccattgctc aggcattaaa agatcattta gctgcctatg aaaagtcgaa
    17941 aagtcttgat acttcaagcc ttaagccaag ccgcccggca ccgccgccgc cgacaagcgg
    18001 gttggtatct gggtcatccc ggtctttacc gcccattgct caggcgctaa aagatcattt
    18061 ggctgcctat gaaaaatcga aaagtcttga tacttcaggt cttaagccaa gccgcccggc
    18121 accgccgcca ccgacaagtg gacaggcatc cgaggcatcc cggccattac cgcctattgc
    18181 tcaggcatta aaagatcatt tagctgccta tgaactgtcg aaaaaggcat aaaaagtaga
    18241 tttaaaatta ttaattactg ttgattgttt agagagttaa aagataaaaa ccgatattaa
    18301 gttgcattgt gataaatgtg attggtaata tcggttttta ttgaaataaa tattgtaata
    18361 aatgatgaaa gagggtaata gagcaagagg ggggtgtttc gttaaccaga gcctgtaatt
    18421 ttttatccat aaatttaccg ctatttgatg ttggatttga catatcaaca tcaggcaacg
    18481 acacaactct atgtacaggc tcttataggt attttttttc tggttagcgt taagggatat
    18541 ctctatttat gccgatatga cgctggcagc cataatatat ctggggatgt catctcgtag
    18601 ctgtttgatg tcaacatctg gactaatatg cttttggaag aactggtctg cgagttgatt
    18661 ggctattaca tccattgtct ggttctttac ttctctactc actcccttgc aaagcatatt
    18721 tattttatca ccgaaacgac catttcctat tatgagatgg ttgatagagc tttgcgctgc
    18781 tatgatattc aacttgagac tgtaatcctt ttcaatgagt ttaaaaactc tttgccgtac
    18841 ttcactggaa atgtttctgc tattttcagc acaatatttg tcaattccct ggttgatcac
    18901 gcgagatggg taaactgttc gctcatctct tttccaggca ttaatatctt tggtactttg
    18961 tcgggcgacg gaggtcaaaa ttcggttgtt cagcgtattt gtggataact tgatagtcct
    19021 ctgacccatt gaacatcgca ggatatcatc agatttcctc cagcaggact gtttctggat
    19081 aaagcttata ccaaatgagt tgaagggtgt tcctgatata tttgctggca tagtaattcc
    19141 ttttatttgt ctatatttac aatgtggcaa gtttataatt attttagtat cgcaacgctt
    19201 tggtgtaaat aatgataagt caataattga tggtttgttg ccaaaatgtt ttgtctgttt
    19261 tattttatgt gtggatgttt atttgttgtt ggcctggata agctaaatac attacagagg
    19321 gagataatat gcccgtcgta ttaaatatct cagatggaag ggcgctgcct gaaagtgaac
    19381 tggaagctct gcgtaatata gcaagaagca atcagagtga tactctgatt gtgggaagtc
    19441 gtagtctgag gctgcaccat gtttcattta tggatgcttt tagtgtggag ccgattcagg
    19501 gcagtcttct tgaccgtctt ggagggaggt gtcgtcgcct tgcagagagt ctggagagac
    19561 agcttaatca tggcaatact ttcctgcagg cgttcagttt atatatggag caggttcgtt
    19621 taaccccatt ctggttggaa gggggcagaa atgcagtgca gaccagaatt aattctcatg
    19681 catttaccgt caatcctgga gattttcctt gctgtgagca acatctgagt tgcccgataa
    19741 cattatgtat accaaaaaca ggcgtttttg ttaaaaatgc actgcattca aaagtctgtt
    19801 ctttatacga taaggatgcc ttgtctgagg ctatccgaca caatgttttt catcccctca
    19861 gtcgtgaagc gttttctcca gaaatgatcg ttggcagaga ggagtgttat tttgatctaa
    19921 ctgatcagcg tttctggatc atttccgggc aggatgtacg tttttgagtg ggggatatta
    19981 atacagactg agttgtactg actgctgctt ttttattcgt cttcgattga caacattatt
    20041 ttgctggttg ttagaatccg ggtcagattg ctgggtttat tctggaatga acatgtagta
    20101 actgtacttg cttttccagc gtgaaatagc gtctgtaatg atgccttttc gccatcggga
    20161 accctggata caggctctct gtatgaaatt tgtcaggtaa gctcctcgga tggggcttac
    20221 cttatggtga tttattttct gaaaatggaa tttattgatg ctttccacat cacaattaaa
    20281 attgtcaggg actgcctgtc atcatattac tgaaacacct gcaagcttta aaagtgaaat
    20341 tacgtcccgg caactggaag atgcacggga aagtggttgt ctgcatataa aaaattgtga
    20401 catggagttc ttgcctcatt accggaagaa atcacagcga taacaataga gaactgtaat
    20461 aagctgaccg gtcttacagg gctaccggtc aatataaaaa gcctgtccgt tattaattgt
    20521 gagaatctga aagtcagtga actgccgccg acacagggca acagtgctgc cgaatctgtt
    20581 ttatcctcat cgttgtcgaa gataattcct gattcgcagt cgatattgtc ctgcagccat
    20641 gtaatcagaa tatccagcac tgtttccgtg gttaatgatt tcatgttgtg aatttccgga
    20701 ttaccagtcg aaagtgggca ggcccggcag atattcagcg ctggcatcca gatgaatgag
    20761 actgacgcca taacgtcggg taagtgtgac aaccagacga cggaacgttt ttgacagccc
    20821 caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
    20881 acctgtaaaa tgaatccatt ctgattcgcc gaagtcactg gtctggtgtg acagcgagta
    20941 caaccaggcg ttgtctgttt ccgtgatatg tgcggtgctg cagcgtatgc cggtaagggt
    21001 tgttaacggt ggtggtgtgc aggttgactg ttggtcggag tcgtccacca cacggagtga
    21061 ataaccgttt tcagcgacct tgttaatcag ttcagtgaga ttaacaccat cgacgtcaac
    21121 gacaatgcgc cccatgttca gcgtctgcgc attaatgctg tcggcttcca gcgtcagggc
    21181 cagtttcatt gtttcgcctc cggatactta cccagggtaa tgttatttac cgttctgtaa
    21241 ttgtcgcggg tcatcaggcc ggtcgccctg cgagccagga ggatatcgat gctgtttatt
    21301 aactgagagc gggtacaggc gctgaatccc ggctggtcgg tagcaccagc gcgtattttt
    21361 ccacgagaaa gttcaccgca tcacacagtg aaatgcctgc ctcaatatgc tgctcaatta
    21421 cacgttcatc ggcaaacggt gtgtcattca gtgtgaggcc atagtgctgg tccagcagtc
    21481 gggacagcag tatctgccag atttcaacag gagacgggcg agaactagcc gcctgcccgg
    21541 gtaatacagg taatgttttc atactgaaga ttttcctgat atgcagatat aaaaatggaa
    21601 aagtggcgtg gtgaaaacac cagaccgtag cagaaggcta ttctggagag ttaatttttc
    21661 atttcgggcg tcggataaac agccagataa acgtaaccac aactgctgag ggtatcggct
    21721 ttgcaggtca gcccttttgc atacagcgtg acggtatgct gatggcgggg attcagttca
    21781 ccgctggtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg gtccaggtgg
    21841 tacgcatctg catcgctgaa caggcctcta ataccggcgc ggtcggcaag gtaatgcaac
    21901 cggttaccct cctgcaccag acgtgccccg aaacagggcg tcacggtgca gggcagcccc
    21961 caccaggggc ggtcgtgatt gtcgtcggga agtgttgtcc cggggagtgt gtctgacacg
    22021 ataaaatcct tacagaaaat cggctaagaa tggtccggta gtggcgataa ttctgctcat
    22081 cagaattccc actcagttca gggtgacgct catcagccgg acatacggac caaaactgtc
    22141 cttacggcgt tctgcaaaca cggccagcac accgggaata tcctgcactt cacgaccggt
    22201 atacgcttca gcactgccgt gccagcggta tttgccggta cagaacggaa aaagacggga
    22261 tgccggatgc tgacggtgaa tacgcatggc ttcgccacgg gtgatgattt tcatagtggg
    22321 atacctctga agacagaaga taaaagtgaa aacaggtgtg atgtggttgt aacggtgacg
    22381 ggttaaagca gaccgtgttc cgcaaaggag aaaacctggc tgccaccgac tatcagatgg
    22441 tccggcaccc ggatatccac cagacccagt gcctgtacca gacgttccgt aataagctgg
    22501 tctgccttac tgggtgtgac ttcaccggac gggtgattgt gtgccagcac cacggcagcg
    22561 gcattgtggt acagggcgcg tttaatcact tcccggggat ggacttccgt gcggttgatg
    22621 gtgccggtga agagggtttc accggcaatc agttgattct ggttattcag atacagcacc
    22681 cggaattctt cacgctccag tcccgccatg ttcagaatca gccattcccg tgccgcacgg
    22741 gtggaggtga aggccacgcc gggttcatga agatggcggt tcagggtttt cagggcccgc
    22801 tgaatgaggc tgcgctcacc gggcgtcatc gctacgggca gaaaggaaag ctgttgcatt
    22861 gtgcttctct ccattcagtc gatgatgcgc ata
//
LOCUS       DAHBHC010000079        22405 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHBHC010000079 DAHBHC010000000
VERSION     DAHBHC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.079"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..368
                     /locus_tag="M2A35_003772"
     CDS             <1..368
                     /locus_tag="M2A35_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCB7200017.1"
                     /translation="VNVTINTTSVDTNHAERDKHLRSADFLNTAKYPQATFTSTSVKK
                     DGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGKIKLKDFNIKTDL
                     GPASQEVDLIISVEGVQQK"
     gene            complement(410..1462)
                     /locus_tag="M2A35_003773"
     CDS             complement(410..1462)
                     /locus_tag="M2A35_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCB7200018.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEIRCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            1687..2607
                     /gene="lpxL"
                     /locus_tag="M2A35_003774"
     CDS             1687..2607
                     /gene="lpxL"
                     /locus_tag="M2A35_003774"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCB7200019.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            2779..4005
                     /gene="mdtG"
                     /locus_tag="M2A35_003775"
     CDS             2779..4005
                     /gene="mdtG"
                     /locus_tag="M2A35_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCB7200020.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4088..4462
                     /locus_tag="M2A35_003776"
     CDS             4088..4462
                     /locus_tag="M2A35_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCB7200021.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(4463..4690)
                     /locus_tag="M2A35_003777"
     CDS             complement(4463..4690)
                     /locus_tag="M2A35_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCB7200022.1"
                     /translation="MRLIVVSIMVTLLSACGSIISRTVPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(4863..7406)
                     /gene="mdoH"
                     /locus_tag="M2A35_003778"
     CDS             complement(4863..7406)
                     /gene="mdoH"
                     /locus_tag="M2A35_003778"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCB7200023.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(7399..8934)
                     /gene="mdoG"
                     /locus_tag="M2A35_003779"
     CDS             complement(7399..8934)
                     /gene="mdoG"
                     /locus_tag="M2A35_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCB7200024.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            9328..10485
                     /gene="mdoC"
                     /locus_tag="M2A35_003780"
     CDS             9328..10485
                     /gene="mdoC"
                     /locus_tag="M2A35_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCB7200025.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(10493..11974)
                     /gene="clsC"
                     /locus_tag="M2A35_003781"
     CDS             complement(10493..11974)
                     /gene="clsC"
                     /locus_tag="M2A35_003781"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCB7200026.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKLVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(11916..12449)
                     /gene="ymdB"
                     /locus_tag="M2A35_003782"
     CDS             complement(11916..12449)
                     /gene="ymdB"
                     /locus_tag="M2A35_003782"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCB7200027.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(12544..12855)
                     /locus_tag="M2A35_003783"
     CDS             complement(12544..12855)
                     /locus_tag="M2A35_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200028.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(12976..13308)
                     /gene="csgC"
                     /locus_tag="M2A35_003784"
     CDS             complement(12976..13308)
                     /gene="csgC"
                     /locus_tag="M2A35_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCB7200029.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(13367..13822)
                     /gene="csgA"
                     /locus_tag="M2A35_003785"
     CDS             complement(13367..13822)
                     /gene="csgA"
                     /locus_tag="M2A35_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCB7200030.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(13863..14318)
                     /gene="csgB"
                     /locus_tag="M2A35_003786"
     CDS             complement(13863..14318)
                     /gene="csgB"
                     /locus_tag="M2A35_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCB7200031.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            15072..15722
                     /gene="csgD"
                     /locus_tag="M2A35_003787"
     CDS             15072..15722
                     /gene="csgD"
                     /locus_tag="M2A35_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCB7200032.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPENYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            15727..16116
                     /gene="csgE"
                     /locus_tag="M2A35_003788"
     CDS             15727..16116
                     /gene="csgE"
                     /locus_tag="M2A35_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCB7200033.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            16141..16557
                     /gene="csgF"
                     /locus_tag="M2A35_003789"
     CDS             16141..16557
                     /gene="csgF"
                     /locus_tag="M2A35_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCB7200034.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            16584..17417
                     /gene="csgG"
                     /locus_tag="M2A35_003790"
     CDS             16584..17417
                     /gene="csgG"
                     /locus_tag="M2A35_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCB7200035.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(17481..17972)
                     /locus_tag="M2A35_003791"
     CDS             complement(17481..17972)
                     /locus_tag="M2A35_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCB7200036.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(18074..18628)
                     /gene="ycdY"
                     /locus_tag="M2A35_003792"
     CDS             complement(18074..18628)
                     /gene="ycdY"
                     /locus_tag="M2A35_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCB7200037.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(18652..19389)
                     /gene="ycdX"
                     /locus_tag="M2A35_003793"
     CDS             complement(18652..19389)
                     /gene="ycdX"
                     /locus_tag="M2A35_003793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCB7200038.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(19444..20382)
                     /gene="ghrA"
                     /locus_tag="M2A35_003794"
     CDS             complement(19444..20382)
                     /gene="ghrA"
                     /locus_tag="M2A35_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCB7200039.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            20616..20703
                     /locus_tag="M2A35_003795"
     tRNA            20616..20703
                     /locus_tag="M2A35_003795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20650..20652,aa:Ser,seq:gga)
     gene            complement(20896..21882)
                     /locus_tag="M2A35_003796"
     CDS             complement(20896..21882)
                     /locus_tag="M2A35_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200040.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(21879..>22405)
                     /locus_tag="M2A35_003797"
     CDS             complement(21879..>22405)
                     /locus_tag="M2A35_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCB7200041.1"
                     /translation="VKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQAALNEFA
                     TLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVA
                     PSIIKENLSLHLTHTVKWTESFDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVVQQRV
                     MHCRKAFSDAKVFK"
BASE COUNT         5879 a         5636 c         5192 g         5698 t
ORIGIN      
        1 aagtgaatgt gacaattaac accaccagcg tcgatactaa tcacgccgaa cgcgataaac
       61 atcttcgcag tgcagatttc ctcaataccg caaaatatcc acaggcaaca ttcacctcca
      121 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
      181 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggtg
      241 gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc aatatcaaga
      301 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
      361 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattccgttg
      421 gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
      481 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
      541 agcgaatctc actacaacaa cctttgtatt tttccgcaca tactggacac tgaataaaca
      601 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
      661 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
      721 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
      781 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
      841 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
      901 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
      961 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
     1021 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca ttgctggcat
     1081 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
     1141 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
     1201 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
     1261 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
     1321 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
     1381 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttagcgcg tcgttggaaa
     1441 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
     1501 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
     1561 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
     1621 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgaataggtt gaaaaacagg
     1681 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
     1741 acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc ggttatctac
     1801 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
     1861 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
     1921 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggcat ggcgtggttc
     1981 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
     2041 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
     2101 ggtgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
     2161 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
     2221 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
     2281 ccggatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
     2341 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
     2401 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccgccagag
     2461 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
     2521 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgc
     2581 ccggaaggcg ttccttcacg ctattaaatc tcccctgccg gatgcttcag aatggcatcc
     2641 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
     2701 ggaagtcggg cgcataatta gtgtgcttat cttttcttct tatgttcacc gcgcctggcg
     2761 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
     2821 aacctgatcg tcgcctggct aggctgtttt cttaccggtg ccgccttcag tctggtaatg
     2881 cccttcttac ccctctacgt tgagcagctt ggcgttaccg gtcactccgc cctgaatatg
     2941 tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc accgttttgg
     3001 ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc cctcggcatg
     3061 ggcatcgtga tggtgttgat gggactggca caaaatatct ggcagttttt gatcctacgg
     3121 gcgcttcttg ggttacttgg cggatttgtc cccaacgcta atgctcttat cgccacacaa
     3181 gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct ccacaggcgg cgttagtggt
     3241 gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt acgtccggta
     3301 ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt ctgcatcaga
     3361 gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt ggtgacatca
     3421 cttaaaaacc cgaaactggt actcagcctg tttgtcacca cgttaatcat ccaggtggcg
     3481 acaggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg taacgtcagt
     3541 aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc tctgctaagc
     3601 gcaccacgac tcggcaaact tggcgatcga atcggacccg aaaagatcct gattacagcg
     3661 ctgatctttt ctgtactgct gttgatccca atgtcttacg ttcagacgcc attgcaactt
     3721 gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc cgtacagaca
     3781 ctgttggttt acaactcgag caaccagatc gccgggcgta tcttcagcta taaccaatcg
     3841 tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc agcgaactac
     3901 ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt tattcaacgc agtctattca
     3961 tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt cgcctttcat
     4021 acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac tcaaagggag
     4081 aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac gcgaagaatt
     4141 tcttgcagac aaccccggca tcgacgccga agatgcgaat gtgcaacagt tcaatgccca
     4201 aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg ccgacgaagg
     4261 ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg tttttgatgg
     4321 cgactatgat gagatagaga tacgccagga gtggcaggaa gagaatacat tacatgaatg
     4381 ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac gcgcagcagc
     4441 tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg atcggcagca
     4501 gtaaagtatc aaaaaccaga gagaatggca gatcaaggat cgtgacataa cgccaggcgg
     4561 agtcacgcac atcccattgc acaccgggat aatattggtt gccatgcccc tgccccggta
     4621 cagtgcgact aataatgctg ccacaggcac tcagcaaggt caccatgatg ctcaccacaa
     4681 ttaaacgcat ttgatacctc tttcgtaaaa aaataccggc ataacgccgg tatttttatt
     4741 ggctcgttgt ttttgccgga tgcggcgtga acgccttatc cgacccacaa aatcttgcaa
     4801 aatcaataaa ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag gcaactacgt
     4861 ttttattgcg aagccgcatc cggtttacgc aatgccagtg gattgagctt tatcccttcg
     4921 taataactca cccatgaaga atatctctcc ggggaattcc agacgcggaa atgcagacgg
     4981 gccatcgtca ccggatcgct tagcagcacc aggcgacgat cgcgattcag cttctctggc
     5041 gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga tttccagcac cttactggcg
     5101 cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa atgacgggtt aaacactgcg
     5161 tgcataaagc catcatcaag ggagcgttga cgattcatct caaggaaccg atcggtatca
     5221 accagcacct gcggcggcga atactcttcc gggatcagga acagtttcca gcgtttggtg
     5281 cgcagaccaa cggtggcacg gctggaaatc accgaaacaa acggtgacag gatcaacgag
     5341 aagacaatcg gtgccagcca gaacaggaaa cgcagatcca accacgccat accaacagcc
     5401 cacactaacc ctaacagcag ctgtgagccg tggcgtttga acgcttcacc ccaggaagtg
     5461 gagtcatcat cacgctgcgg tgaattccac accacttccc agccaaggaa cgcgctgacg
     5521 acgaagaccg tatggaacag catgcgtacc ggagccagca gcacggaaaa cagcacttcc
     5581 agcagcaacg ataatgtaac gcgccagaag ccgccgtatt ctttcgttcc tttgcaccag
     5641 ataagcaaaa tgctcaataa cttcggcagg aacaacagca ccatggtcga agcaaaaagt
     5701 gcaatcgcca gctcaggacg ccactgcggc cacaccggga acaactgtcg tggttgcagg
     5761 aagtattgcg gttcggtcaa cgcatgtact acctgcaatg cagtagagag cgcgaggaac
     5821 ataaaccaca gcggagcgga gagataagac atcacgcccg tcaggaatac cgcacggtga
     5881 accgggtgca tacccttcac caggaacaga cggaagttca tcaggttacc gtggcaccag
     5941 cggcggtcac gttttagctc atcaagcaag ttaggcggca attcttcata agaacccggg
     6001 agatcgtaag caatccagac cccccaacct gcacggcgca tcaacgccgc ttccacgaag
     6061 tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg gcagcggagc cagtgcgcag
     6121 tgctcgataa acggtttcac gcggataatc gcgttatgtc cccagtagtg cgactcgcca
     6181 agttgccaga agtgcaaacc ggctgtaaac agtggcccat acacgcgggt cgcgaactgc
     6241 tgacagcgcg catacagcgt atccataccg gacgctttcg gcgacgactg aatgatcccg
     6301 gcgttcgggt tggcttccat caggcgcacc aggccgcata aacaatcacc ggtcattacc
     6361 gagtcagcat ccagcaccac catgtagctg tactggctgc cccagcgacg gcagaagtca
     6421 tcgatattac cgcttttacg cttcacgcgg cgacggcggc ggcgatagaa aatctgacct
     6481 tcgccaccga cttcagcgat aagctccatc caggcttttt gctctgcgac gcagatatcc
     6541 gggttataac tgtcactaag aatgtagaca tcaaagtgtt tggcattccc ggtggctttt
     6601 actgattccc acgttgcacg caggccagca aaaacacggt tcacgtcttc gttacagata
     6661 ggcatgatca acgccgtgcg atgctccggg tttaatggtt catcgccaac tgttgacgca
     6721 gatatactgt atttatcgcg accaataagc agttgcagga agcccattaa cgccgtccag
     6781 aatccggcgg acacccaaca gaacagtacc gcaaagagga tcaggatacc ggtttgcagc
     6841 atataaggca gaagctgcat aaaggaaacc cacaaatcct gaccaaccat atccatagga
     6901 ttaatcagcg cccaaccctg ataaggaaga atggtcttca tataccaggt cgcgacgaca
     6961 gtttgcgcga gcgtcaggat caacagaatg taacggcgga tggtaccgac ggtacgccac
     7021 ttttgctcgc tctcctgctc ttctttggtc aaacgagcca gatagcgcgg cgtgacatcg
     7081 cgtccacgca ggcgatccca gaaacggcct accgggttgg tacgccacgg gtcgggaaac
     7141 atcgaggagc gttttgcttc tggcatcgcc ttcagctgat cgcgcccttc gtcgtcttta
     7201 attaactgtc catcagcaag tgaatctggc caggcttgtt ccagacgcgc ctttaccgag
     7261 ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt cggcatccag cgcctgatga
     7321 acggcgcgga tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat gggcattgcg
     7381 tcaatgtact cagttgtctt attcattggc aggtaactgg tagctccagg tttcactcaa
     7441 cgtctgatcg gcattcacca gcgcagcacg catttcagtg gttttcttgg catctttcac
     7501 tttcacacgc atcaccagac gccagccttt ggtaaccggg ttatagcgca ccgtgctttc
     7561 aactatctca ccattatcac caatgctggt ttgcgctgtg accggggtat cctctggcag
     7621 ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg atagtaccgt caggctggcg
     7681 aatcaggttc gactgcttca catcccccgt tgaacgacgc gtttgttgca cccatgcgtt
     7741 atctggcgca tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt taaagttcat
     7801 ctctttaccc ggctccggca gctgatccgg cgtccagtaa gcgacgatgt tatcgttggt
     7861 ttcatcgttg gttggaattt ctaccagctc aacgctgcct ttaccccact cccctttcgg
     7921 agtcacccat gcgcttggac gaagatcgta acgatcatcg agatcttcaa agcgggagaa
     7981 atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt tccatagaga agctgctgac
     8041 cgctaaatgt ttcgggttat tcaacggacg ccagatccat tcgccattac cggcatggat
     8101 agagagaccg ttagagtcgt gcaactccgg acgatagtta tttgcaggcg acggttggtt
     8161 cggcccaaac aggaacatac tggttaacgg tgcaaccccc agtttgccga ctttatcgcg
     8221 cagatagatt ttcgactgca catccacaac cgtgtcacgc cctggcataa ctacgaattt
     8281 gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac gtttatcagt
     8341 cggttttgga cgctcgatcc agaactcttt gaagcgtgga aattcttcac ccgatggcaa
     8401 ggcggtatca attgccaggc cgcgggcaga aaggccataa acctgacctg caccaatcac
     8461 gcggaaatag ctggccccga gcatgctgac gatttcatcg tttttatctt tgctgttgat
     8521 cgggtaaagc actttaaaac cggcaaaacc aaggtctttt accgtgtctt tgtcatgctg
     8581 aacatcgccg aaagtgaaat aatccgggct gtatttgatt cgtttgactg cggtggcagt
     8641 cacttcattt attttgaccg gggtatcgaa gtacataccc tgatggtaga actcgagttt
     8701 gaatggggtc ttcagattgt tccagtacgc tttgtcatga ttaaactgga tctgctgata
     8761 gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg cctcatagcc
     8821 tttcccggct aaggattgag cttgctttgc gacatcatca atactgaaag cccagcttga
     8881 agatgtatac agggttaaca ttactgcagc actcaaccaa cgcattttca tcatttgtag
     8941 tttatgtttc ataattagta agcacttccc cctttgtgtg cttatatcga tccgatctat
     9001 tttaatggat aaccagccat tccgacaaca gaatcccggt atcgttcagc acgctggctc
     9061 atggattaag cacctgattg caaccgtttt cactttccgt gcttctccta aagacagaag
     9121 catcgacttc gtgtagggtt tgccagcaat tttatttatc aaccgtctta cagacgcttc
     9181 cattctggtt agtggtagcg atgatgctat gcataaaaac cttgaagcct gcgcctggcg
     9241 cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca taacccgaca gggataagac
     9301 gaaaaatccg agattagtta accatatatg aatccagtac ccgcgcaacg tgaatatttc
     9361 ctcgactcca tccgcgcctg gctgatgttg ttagggatcc cttttcatat ttctttaatc
     9421 tattcgagcc atacatggca tgtgaatagc gccgaaccgt cattgtggct gacccttttt
     9481 aatgacttca tccactcgtt ccgcatgcag gtatttttcg ttatatccgg ctacttttcc
     9541 tacatgcttt ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt cgaacgtgta
     9601 ggtatcccga tgttaacagc catcccctta ctgacattgc cgcaatttat tatgctgcaa
     9661 tacgtcaaag ggaaagcgga aagttggcct gggctgtcat tgtatgacaa atataatacg
     9721 ttggcctggg aattaatatc acatctgtgg tttttactgg tgttagtggt catgacgacg
     9781 ctgtgcgtat ggatatttaa acgcatcaga aataatttag aaaattctga taaaacgaat
     9841 aaaaaattct cgatggtaaa actatcggtg atttttttat gcctcggcat cggttatgcg
     9901 gtaataagaa gaacgatttt tattgtgtat ccgcccattc tgagtaatgg catgttcaat
     9961 tttattgtca tgcaaacgct gttttatttg ccgttcttta tcctcggcgc actggctttc
    10021 attttccctc atcttaaagc cttgtttacc acgccgtctc gtggctgtac ccttgcagca
    10081 gcattggcgt ttgtcgctta tttactcaac cagcgctatg gcagtggcga tgcctggatg
    10141 tacgaaaccg agtcggtgat caccatggtc ctcggtctgt ggatggtgaa tgtggtcttc
    10201 tcctttggcc accgtttgct taacttccag tcagcgcggg tgacttattt tgttaacgca
    10261 tcgctgttta tctatctggt tcaccacccg ttaacgctgt ttttcggcgc atacattaca
    10321 ccgcacatca cctccaactg gcttggtttt ctctgtggcc tgatatttgt agtagggatt
    10381 gcgataattc tgtatgaaat tcatttgcgc atcccgttac tgaagttttt gttctctggt
    10441 aaaccggttg ttaagcgtga gaacgataaa gcaccagccc gttaagccac atttacaata
    10501 accattccac gggcaatatc gacgccagtc tgaccataac ccgcttccag aaactggtgg
    10561 cgggttcttt tttgagaata atctctttct ttgcatgacg atcaacccag ttgatccgtc
    10621 cccacctgtc cagacggagc tgccaggccg catcatactg gctctgaata aagcgtttat
    10681 caattaactg tgccagcgtt tcgctctcta tcacgaagcc catttcagta ttgagcaatg
    10741 ttgaacgcgg atcgaaattg aaagagccga taaacaccgt tttaccatcg atgctaaagg
    10801 ttttagcatg caggctggct ccggaattac cggttatgcc gcgatcgtgt aacgtactac
    10861 tttgttcacg cgtcggcttg agttcatata attccacgcc atagcggagc aattttttgc
    10921 gccagcgcgc gtatccggca tggacgacag caacatcgtt agcggcaaga gaattggtta
    10981 ggatcgcaat ctttacccct tttctcacca tccgtaagag ttgcgccaca cctgcgcgtg
    11041 tcggtacaaa ataggaagag ataatatcga tgcgttcact gggtgagccc atgatatcga
    11101 acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc gccggatcat
    11161 cacttaataa acgtgttttc gcccagataa gcggcaacgt tccatcaacc agatgattta
    11221 taaacggact ggattccatt ttgcgtaagt aacgatgcgt catagcatcg ttatgccagg
    11281 aggcgggtaa ctcgatgcga tccgccattt caccttccgg gacatccagc acttgctgta
    11341 agggtgaaac caatttgcaa taccagtagc gggcgaaatc atcggcaacg tcctctacca
    11401 cgggccctat tgccataaca tctaaatccg aaaaaagtgg ctcttcccct gccccaaaat
    11461 aggcatcacc aatatttcgc ccccctacca gcgtcaccac gccatcgaca gtgaaacttt
    11521 tattgtgcat acggcgattg agacgggaaa agtcggtgat ataaccaagc ggacgcagca
    11581 agcgaaacga gaaaggatta aaaagccgga cttcaatgcg gggatggctg tcaagcaagc
    11641 gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg acgcgaacgc
    11701 cacgctttgc tgcggctaac agggcggaaa acagtaaccg acccgacata tcgtcctgcc
    11761 agatgtaata ctgaacatcc agcgtatgct ctgccatttc ggccagacga taccgggcgg
    11821 caaacgcatc cagacttttc tccagaggga aaagaccaca ctgaccggga tgttgcgaac
    11881 acagtggcag caccgcgctc gccagccggg gcaaatcatt catctccttg ttgggtaagg
    11941 agtctttcgt agaggtgggc gttttcttca tcataacaga caaagtatac ctgttcgggt
    12001 aaagcgtgac gggtaataaa ttctgaaacg gttttaactg cgatttcagc cgctgccgca
    12061 cgagggtaac cataaacccc agtactgatt gcaggaaaag ccactgacgt atagctgttt
    12121 gccgccacca gtcgtaggct attgagatag gcatcctgca aaagctggtc ttcgttttgt
    12181 tcaccaccac gccagactgg cccgacggtg tgcactacgg ctttagcggg aagagcgcct
    12241 gcaagcgtaa taacggcatg ccccgtaggg caatcgccct gctgttgcct gacttttaaa
    12301 caggcatcca gcagggccgg acccgctgcg cgatgaatgg ccccatcgac gccgccgcct
    12361 cccattaatg acggattagc cgcattcaca atcacatcaa cggccagttt ggtaatatca
    12421 ccctgcacaa catgaatacg cgttttcata acgcctcctt acaccttctt gtttcttaag
    12481 tgtacagcag gctttttggt taaatcgcat ttttctgctc agcaacgtgc gttgttcaga
    12541 aacttagtta tattcgatgt tcattacagc cagctttttc tgtttatcaa gatactgcac
    12601 ttttactgag gctatttgcg cgtttttaac attaccgctc attaacgctt tgctggaata
    12661 ggttttgccc ggaacagaac cattctgtgg gcagttcatt tctacgggtg atgactgggt
    12721 gctgacgtca catgccggtt ccacaatttg gccataaaaa tggatcaccc ccccttgcgc
    12781 ggtgcttcct gcgatggcaa taaaaggaaa aagcaaactg ccgagcataa gagagtttaa
    12841 aaaaggtctg aacatttatt ctatttcctc aatgattagt catccttgag ggttgtgtta
    12901 tccatacttt caggttggct gattcgcagc agaccattct ctccagattc atcttatgct
    12961 cgatatttca acaaattaag acttttctga agagggcggc cattgttgtg ataaatgaag
    13021 tgactgtcca tcagaaacag taacaactat tttcacccga tcttccgggg aaatatttaa
    13081 actcaacttc gtcaaagcaa taggttgatt agcaggcaat gagagggtct tttcttgctt
    13141 cgtctgactt tgccctgaac tgccttcgcg cagggacaat atttgtactc tgcacagaca
    13201 agattgagta agagtgactt caggaataat ggtatacaca tccccttgct gggtcgtatt
    13261 aaaggttatc tggctggaaa gtgccgcaag gagtaataac gcattcatat tcttctcccg
    13321 aaaaaaaaca gggcttgcgc cctgtttctg taatacaaat gatgtattag tactgatgag
    13381 cggtcgcgtt gttaccaaag ccaacctgag tcacgttgac ggaggagtta gatgcagtct
    13441 ggtcaactgc agcaccgttg ccaccaccga actgtttaac agtcatttca gaatttttgc
    13501 cgttccactg atcaagagtg gcgctgttac cgaagccacg ttgggtcaga tcgattgagc
    13561 tgtcatctga gccctgacct acatctgcac cattaccacc gccatgctgg gtaatagtca
    13621 agtcagagtt acgggcatca gtttgcagag caagtgcaga gttaccgcca ccgtactggt
    13681 aaatgttcag ctctgaattt gggccgctat tattaccgcc accaccgtgg ttaccgccgc
    13741 cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt gctgcaattg
    13801 ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa gtcgaatgga
    13861 aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac aattgccgtt
    13921 ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg gataatcatc
    13981 gcagtattac cataagcacc ttgcgaaata ctggcatcgt tggcactgcc cgcctgatca
    14041 atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct actaccttct
    14101 tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact attattcgtc
    14161 ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc atttaccgcg
    14221 aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc aggcgcaccc
    14281 agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg gacctggtcg
    14341 tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctgta ttttgttaac
    14401 gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac ccgcgcgttg
    14461 tatttttctt tttttgttca taccaaagta ttaaaaccca cgtaaatacg gtgttatcta
    14521 cgcaaaaaat attttgtttt cttttaaatc tccgttttcc gctatcaaaa atcaccagac
    14581 agtcattttt cttgcccgtc gctgattgct gcgatatgtc ttgcgcacaa gccgtgacaa
    14641 acctcgtctt tttgcagtaa aaaattgtcc acggaggtgc ggagaaaaaa caagaacgtt
    14701 ttacatgacg aaaggactac accgaaatat tttttatatg tattattagt aagttatcac
    14761 catttgtatg attttttaaa attgtgcaat aaaaaccaaa tgtacaactt ttctatcatt
    14821 tctaaactta ataaaacctt aagattaaca ttttaatata acgagttaca tttagttaca
    14881 tgtttaacac ttgatttaag atttgtaatg gctagattga aatcagatgt aatccattag
    14941 ttttatattt tacccattta gggctgattt attactacac acagcagtgc aacatctgtc
    15001 agtacttctg gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa taaaaaaagc
    15061 ggggtttcat catgtttaat gaagtccata gtattcatgg tcatacatta ttgttgatca
    15121 ctaaaccgtc tttgcaagcg acagctctct tgcagcacct taaacaatcg ctggcaatta
    15181 caggaaaatt acataatatt caacgttctc tggacgatat atcttcaggc tctattattc
    15241 ttctggatat gatggaagca gataaaaagc ttatccatta ttggcaggat actttgagca
    15301 ggaaaaacaa caatatcaaa atattgttgc taaatacgcc tgaaaattac ccgtaccgcg
    15361 acattgaaaa ctggcctcat atcaacggcg ttttttatgc catggaggat caagaacgtg
    15421 ttgtcaatgg gttgcaaggc gtcctgcgcg gcgaatgcta ctttacgcaa aagcttgcca
    15481 gttacctgat tacacattct ggtaactatc gttataacag cacggaatca gccctcctta
    15541 ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg
    15601 ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca tctttataat cttttcaaga
    15661 agatagccgt aaaaaaccgg acacaagcgg tttcctgggc aaacgataac ctcaggcgat
    15721 aaagccatga aacgttattt acgctggatt gtggcggcag aatttctgtt cgccgcaggg
    15781 aatcttcacg ccgttgaggt agaagtcccg ggattgttaa ctgaccatac tgtttcatct
    15841 attggccatg atttttaccg agcctttagt gataaatggg aaagtgacta tacgggtaac
    15901 ttaacgatta atgaaaggcc cagtgcacga tggggaagct ggatcactat aacggtcaat
    15961 caggacgtta ttttccagac ttttttattt ccgttgaaaa gagacttcga gaaaactgtc
    16021 gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta
    16081 ttaagtacgg gcgatttggc gcatgatgaa ttctaaataa aaaattgttc ggaggctgca
    16141 atgcgtgtca aacatgcagt agttctactc atgcttattt cgccattaag ttgggctgga
    16201 accatgactt tccagttccg taatccaaac tttggtggta acccaaataa tggcgctttt
    16261 ttattaaata gcgctcaggc ccaaaactct tataaagatc cgagctataa cgatgacttt
    16321 ggtattgaaa caccctcagc gttagataac tttactcagg ccatccagtc acaaatttta
    16381 ggtgggctac tgtcgaatat taataccggt aaaccgggcc gcatggtgac caacgattat
    16441 attgttgata ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc
    16501 ggacaaacct cgaccatcca ggtttcgggt ttacaaaata actcaaccga tttttaagcc
    16561 ccagcttcat aaggaaaata atcatgcagc gcttatttct tttggttgcc gtcatgttac
    16621 tgagcggatg cttaaccgcc ccgcctaaag aagccgccag accgacatta atgcctcgag
    16681 ctcagagcta caaagatttg acccatctgc cagcgccgac gggtaagatc tttgtttcgg
    16741 tatacaacat tcaggacgaa accgggcaat ttaaacccta cccggcaagt aacttctcca
    16801 ctgctgttcc gcaaagcgcc acggcaatgc tggtcacggc attgaaagat tctcgctggt
    16861 ttataccgct ggagcgccag ggcttacaaa acctgcttaa cgagcgcaag attattcgtg
    16921 cggcacaaga aaacggcacg gttgccatta ataaccgaat cccgctgcaa tctttaacgg
    16981 cggcaaatat tatggttgaa ggttcgatta tcggttatga aagcaacgtt aaatcgggcg
    17041 gtgttggggc aagatatttt gggatcggtg cagacacgca ataccagctc gatcagattg
    17101 ccgtgaacct gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg gtgaacacca
    17161 gtaagacgat actttcctat gaagttcagg ccggggtttt ccgctttatt gactaccagc
    17221 gcttgctgga aggggaagtg ggttacacct caaacgaacc cgttatgctg tgtctgatgt
    17281 cggctatcga aacaggggtc attttcctga ttaatgatgg tatcgaccgt ggtctgtggg
    17341 atttgcagaa caaagcagaa cggcagaacg acattctggt gaaataccgc catatgtcgg
    17401 ttccaccgga atcctgacag ataaaaaagc cggaattatc atccggcatt ttactgttta
    17461 agcgaaagcg tgagtgcccc tcacgcttta tttttgattt cctgctcacg gtgagccgtc
    17521 tttgcactac gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc aaataccagc
    17581 ccaagcgcca gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat
    17641 gcgcctataa acgtccccgg aacaaatgaa agcagcagct gtttggcctg aatacacatc
    17701 agaaacgcga caataccggt tatgacataa ccgagaattt ccagatgtgg tgccagtgca
    17761 ctaccgtaaa taatgaccat cgcccacacc acgccactaa gcagggttgc agcggagatc
    17821 gccagccctt tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc caggaagcct
    17881 gcccagctta gtaagcccag ggaaacagcc acccatcccc agataccgga gagaatgcct
    17941 gttgtgattg caatagagag aagtatgttc atgccgcgca tgatagcaga aaacgcggcc
    18001 atgaatgaga tcgagcacac attttatgca acaacaaatt atctgttgca gttatgagtg
    18061 acgatgatca catttactct tcagaatctt cttccagctc gtcccacatt gcactaatgg
    18121 catcacgggt tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt
    18181 tgccgaggaa cgcaccacac cagggtaaca gatactcact gaacagtgtt tccagtgctt
    18241 cgctctcatc ttccgttgac tgatcttcca gccaggaagc cgcaagcagc aatgtgccga
    18301 tgtgatcggc tggcgtatcc gccagtggca tccctcgttc ggaaagaaaa gcgcgcactt
    18361 ccgcttccgt tgcgtcctca acccatgcgc tacgatatgg cggcacggca cattcatcgc
    18421 cgataaacaa cgcattgtaa tcggcagaga cttgcgccat atcacagctt ttctgtaaac
    18481 gcgtcagtaa ctcatcctgc tccagtggcc agttcgcagc cagtttcccc tcacgaatca
    18541 gggtaaacag cggcaccagt aaaggatctt gcggttggcg gtaatagagc gaacccagca
    18601 cacgacagag gatagaaaac tcgttcatta atatatttcc attactagta attaaaggtc
    18661 tgcaaattcc gcaatcggtg ccataccgcg agattcaagg aagttcagta agcggcgcgg
    18721 agaaacattc aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt taagacactc
    18781 ttcaaattcc cccatggtaa acgcggtgtg agaatccgag cctaatgcca cccaacctcc
    18841 agcatcacgt accgctgcgg ctaccgcacg acagttgtct tcactgccct tacgtgagtg
    18901 taaaaatgaa gaattattga tttccagcgc cacctgatgt ttcgcggctg cttcagcaac
    18961 ggctttcaca tctatttcat atttgggatt tccgggatgg cttattatat gcacattgcc
    19021 gctggcgata gtggcgatca tcgcttgtgt attggtcgct ttgtcatgtg gtgcaaaaac
    19081 cggctcatga aaaccggcaa taatcagatc cagcgagtca aacattttac cgctgcaatc
    19141 aatttcacca tcaacatttt taatgttagc ttcgatgccg cgcaggatcc ctaccccatc
    19201 aaccactcgc ggccagatac gcatgttaat gaagtgccag tgatgcggag catcttccat
    19261 atccgggcca tgatcggtga tcgcaaaaag tttaatgccc ttttgtttgg cctgggcaat
    19321 gtaatcactc aatgtgctat atgcatgtgt gctggcaacg gtatgcatat gaaggtcgac
    19381 gggatacata actctctcct gtattcaatt cccgccaagg atagcaggaa tcctgatgct
    19441 ttattagtag ccgcgtgcgc ggtcgacttg cccgcagacc ctctcccctt tttcgagctg
    19501 ggcaatggtg cgagaaatgt actccacagc ttcagcggga cgggtaatcg cggcgacatg
    19561 tggtgttatc gtcacgcgtg gatgttgcca gagcggactt tcaggcggta agggttcacg
    19621 attaaaaaca tccaacattg cgcctttaac tttgccgcta tccagcgccg cgagcaggtc
    19681 atcttccaca acatgaacac cacgcgccag gttgaggaga tacgcgccat ccggtaattt
    19741 ttcgagtaat tgttgattaa taatgccgac ggtttcaggg gtattcggta acaaattaat
    19801 caatacccga cattggctca gaaatgcaga cagttcttcc cgtccggcaa agctttgcac
    19861 gccaggccac gatttacggg ttcgactcca gcaacgcagc ggaaagcgcc aggtttgcag
    19921 actctgagca actttactgc ccagtacgcc tgcgcccaaa atgccgatgg taaaatcttc
    19981 ccgatgatat tcaggcagcg gttgccaatg cgaactattt tgctggatgc gataatcgtc
    20041 aaaacgtcga aaccaatgca gcacctgact gacagcatat tcctgcattt gctcgcccat
    20101 accggtatct tccaggcgaa aaagtggaac agaagggttc agcatttcag ggtgtgcctg
    20161 tagcttgctc aaaatagaat caacaccggc cccgagtgcg aacaccgctt taagatcgcg
    20221 ccctgccagc atttcaacag gaggatgcca gactaaagca taatcagcag agtcattatc
    20281 tccgcttttc catgctctga ctcttgcctg aggaatagct ttgcgcagtg cctcaatcca
    20341 ccattgggta tcgaacgttg ggtgataaaa gatgatatcc atactgactc ccgaaaagcg
    20401 ttgtgcgaaa tttattcgca cttatcgtta tgatctacaa aggccaccag cataacaaat
    20461 ccgtggtcgg tggcaaaaaa agcagatttc gcttactaaa accacacatt gattgaagtt
    20521 tgaataaacg cgcgattttt tcaaaaagtt tgttgacctc aggtcatgat ttccctaaat
    20581 tagcgcccgt tccagcaaga caggaacgac aatttggtga ggtgtccgag tggctgaagg
    20641 agcacgcctg gaaagtgtgt atacggcaac gtatcggggg ttcgaatccc cccctcaccg
    20701 ccatatttaa agaagagccc gtacgaaagt acgggctttt ttttcgtata ttgcacacac
    20761 cgggtgcccc cgcccacgtc tgttagggcg aggggaaatt gtgcacatca actgtctggt
    20821 tactcagtca acaatcaaaa tgcgcaagat agtagagaac atatatgcgt tatctggtca
    20881 cttcctgtaa cttcctcact tattaaaatg cccccaacgg aaatacatgt cacatgccat
    20941 cataaaatcg ttatgccaga ggaatcgaca atggaaaaaa tagtaaatta agcacggtac
    21001 atagcagagt aaaaaatagg cttcaaacca aatccgccat tccacaagcg ttgccaatac
    21061 cgacaccagc gtaatccaca tcatttcaat aaccagcaat atgaataacc gaagccaaag
    21121 cggcttggca aaaaaggtcg acaacgcatt ttcactggag aaaaacagat cgatttcctg
    21181 caaaaaaagc ctgcccagaa aagcgtaccg cttggagaag cgcagaaaaa acagattaat
    21241 cgccaacact acgccacaaa gaatgagcaa tgcaattatc gatttaaacg ttaaccactg
    21301 cccctctgtc acaaaagaat cactgttacg caacgggctg atagtaagca ggttaacaac
    21361 cgttgagatg cacaccgaaa gcagcacata gcgagtcaga cttttatatt ttactttttt
    21421 cgctactgaa atagcgacct gcttgacata actggtgcgg atcatccgaa acagcagaaa
    21481 gatgaccaac ggcccaagta acgaaatcag gaacagcgca tataacgagg gtagaagctg
    21541 gtatagcccc caactcattg cactcaacat gattaacgac agaaatcctg ccaccagagg
    21601 ttgtgtaaga aataattcta ttttgtggtt ctgctttttc ttaaaccacg aaaaatcgat
    21661 attcttcgtg acgcatttat aagcccagta cgccttgaga tcaaaaataa aattatacag
    21721 caccagggcc agaatcccac taactacacc aatatcctct gatggaaaag taaaccctgc
    21781 attcttccaa taaaagccta tcagaataag tccaatagca taaatgtata tcagatttcg
    21841 tttatcctga aagcgaataa aagttaaata atcaggaatc atttgaatac ctttgcatca
    21901 ctaaatgctt tacgacaatg cataactcgt tgctgaacca cgccagcaaa atcatttaac
    21961 atattcccca aatagggctt attacttatt tcaaggaaag ctaccggggt tggcatattc
    22021 agaaatgtat cgaaagattc agtccatttc accgtatgag ttaagtgtaa tgagagattt
    22081 tctttgataa ttgacggagc gacctcgctt tttgctgtta cgttcatcag aacctgatgc
    22141 tctggcgaag cgatatccag tccggcaaga taatcgcgca ttgcctgaac gccgtcttcc
    22201 atcaaacgcg tgtgccaggc accgctcaca ccgagtttaa ccggttcgta tccagcggcc
    22261 atcagcagcg tggcaaattc attcaacgca gcctgcgttc ccccaataac ctgctggcgc
    22321 ggcgtgttat cacagctaat atccagcgca atgcctgatt ccgtaatcat cgtctgcagt
    22381 tgttcgcggt tgatgccttt aaccg
//
LOCUS       DAHBHC010000080        22328 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHBHC010000080 DAHBHC010000000
VERSION     DAHBHC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.080"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..133
                     /locus_tag="M2A35_003798"
     CDS             <1..133
                     /locus_tag="M2A35_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCB7200042.1"
                     /translation="ICGSCRVQLLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(130..672)
                     /gene="zapC"
                     /locus_tag="M2A35_003799"
     CDS             complement(130..672)
                     /gene="zapC"
                     /locus_tag="M2A35_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCB7200043.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(846..1856)
                     /gene="pyrD"
                     /locus_tag="M2A35_003800"
     CDS             complement(846..1856)
                     /gene="pyrD"
                     /locus_tag="M2A35_003800"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCB7200044.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1967..2704)
                     /locus_tag="M2A35_003801"
     CDS             complement(1967..2704)
                     /locus_tag="M2A35_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCB7200045.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(2670..3185)
                     /locus_tag="M2A35_003802"
     CDS             complement(2670..3185)
                     /locus_tag="M2A35_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200046.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(3193..3735)
                     /locus_tag="M2A35_003803"
     CDS             complement(3193..3735)
                     /locus_tag="M2A35_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200047.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(3747..4817)
                     /locus_tag="M2A35_003804"
     CDS             complement(3747..4817)
                     /locus_tag="M2A35_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200048.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(4808..7408)
                     /locus_tag="M2A35_003805"
     CDS             complement(4808..7408)
                     /locus_tag="M2A35_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCB7200049.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGGSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(7433..8134)
                     /locus_tag="M2A35_003806"
     CDS             complement(7433..8134)
                     /locus_tag="M2A35_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7200050.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(8217..8759)
                     /locus_tag="M2A35_003807"
     CDS             complement(8217..8759)
                     /locus_tag="M2A35_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200051.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            9115..9690
                     /gene="ssuE"
                     /locus_tag="M2A35_003808"
     CDS             9115..9690
                     /gene="ssuE"
                     /locus_tag="M2A35_003808"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCB7200052.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            9683..10642
                     /gene="ssuA"
                     /locus_tag="M2A35_003809"
     CDS             9683..10642
                     /gene="ssuA"
                     /locus_tag="M2A35_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCB7200053.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADTRAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            10639..11784
                     /gene="ssuD"
                     /locus_tag="M2A35_003810"
     CDS             10639..11784
                     /gene="ssuD"
                     /locus_tag="M2A35_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCB7200054.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            11795..12586
                     /gene="ssuC"
                     /locus_tag="M2A35_003811"
     CDS             11795..12586
                     /gene="ssuC"
                     /locus_tag="M2A35_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCB7200055.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            12583..13350
                     /gene="ssuB"
                     /locus_tag="M2A35_003812"
     CDS             12583..13350
                     /gene="ssuB"
                     /locus_tag="M2A35_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCB7200056.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13557..16169)
                     /gene="pepN"
                     /locus_tag="M2A35_003813"
     CDS             complement(13557..16169)
                     /gene="pepN"
                     /locus_tag="M2A35_003813"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCB7200057.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            16435..17637
                     /gene="pncB"
                     /locus_tag="M2A35_003814"
     CDS             16435..17637
                     /gene="pncB"
                     /locus_tag="M2A35_003814"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCB7200058.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            17806..19206
                     /gene="asnS"
                     /locus_tag="M2A35_003815"
     CDS             17806..19206
                     /gene="asnS"
                     /locus_tag="M2A35_003815"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCB7200059.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            19808..20896
                     /gene="ompF"
                     /locus_tag="M2A35_003816"
     CDS             19808..20896
                     /gene="ompF"
                     /locus_tag="M2A35_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCB7200060.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            21080..22270
                     /gene="aspC"
                     /locus_tag="M2A35_003817"
     CDS             21080..22270
                     /gene="aspC"
                     /locus_tag="M2A35_003817"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCB7200061.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
BASE COUNT         5511 a         5624 c         5454 g         5739 t
ORIGIN      
        1 catttgtgga agttgccgtg ttcagctttt agaaggcgaa gtcacgccgc taaaaaaatc
       61 agcaatgggc gatgatggca ccattctttg ctgtagctgt gtaccgaaga ctgcacttaa
      121 gttggcgcgt tagactgcct gttcgaggct gaagctgtca acattaacct gcggtttcag
      181 cctgtcgttc atgattttaa tggcgtcgcc caactgcatc gcgcgacccg caataacaac
      241 gcagggttgt gccagcaggc atagtgcggc attttcgcca gattcaacga ccagcaaatt
      301 gacctgctcg tgagtatcgc tcaaccaaac gcaggccgca tcgcccggca tgggcaccca
      361 catttcacca tgggaaacaa aatgccagct tttcggcatt tgcggtttga gatagcgaat
      421 tgctaccagt gcattcagca ccagttcggc tttttgttct ttagataaat taaaatcgcg
      481 gcacttttct tcaaaagaga aatagagcgc ggcgtcgtca acgcaaaagc ctgcgggaga
      541 aaaggcatct ggagtcaaca tcttgcgcgc aaaacgtgag cgaaatagca taccattggc
      601 taaatcgagc atcattcgat cgtgctcttc atcgtaatac caacgccagt tatcgtctgg
      661 tttaattcgc atctgcctct cccctaccct ataaaaccgc agcaacacaa tccattgtcg
      721 cttcgcttaa taacctcaac aataaaagta caaaatgtct aaataagcaa cagcgatgaa
      781 tataaaacaa ccagggctga aaataaagcc ctgattatct tataaagagg aattcgaaga
      841 aataattaga tatgggtaac gatttcttta atcagcggcg gacctttaaa aataaaacca
      901 gaataaattt gcaccagtga ggcacccgca gcaatctttt cacgcgcagc gataaccgag
      961 tcgatgccgc caacaccgat gatcggtaag cgaccgttta attccagtga caagcggcga
     1021 ataatttcgg tgctttttaa ctgaagcgga cgaccactta agccaccggt ttgatcgcaa
     1081 tttttcattc cctgaacaag agaacgatcg agtgtggtat tggttgcaat aacgccatca
     1141 atattatggc gaactaaact atcggcaacc tggatcaatt cttcttcaga aagatccggc
     1201 gcgatcttca ctgcgatcgg cacatattta tggtgcatcg cttgcaaatc attttgctta
     1261 tttttaatcg cggttaagag atcatccagc gcttcaccat attgcagcgt gcgtaatcct
     1321 ggggtattcg gcgatgaaat attgatggcg atatatcccg cataggcata gattttttcc
     1381 atacaaatca gatagtcatc tttgccctgc tccactggcg tatctttatt tttgccgatg
     1441 ttaataccca ggacgccgtc ataatgggcc ttttttacgt tctctacgag gttatcaacg
     1501 ccaagattat taaagcccat acggttgatc aaaccttcgg catctaccag acgaaagaga
     1561 cgcggcttgt cattacctgg ctgtggacgt ggcgtgacgg taccgatctc gatcgatcca
     1621 aatcccatcg cgcctaacgc gtcaatgcac tccccgtctt tatcaagacc ggctgccaga
     1681 ccaagcggat ttttaaacgt caggcccatg cagttaacag gtttcgcagg cactttctgc
     1741 cgcaccagtg cttcaaacgg cgttcctgta atacggcgta attgctgaaa agtaaactca
     1801 tgagcgcgct ctggatcgag ctggaaaagg gctttacgaa cgaaggggta gtacatgaac
     1861 tctcctggat tcccggtgtg caaaccgggg gcgtattatg tgcgagttcg gaccaaaagg
     1921 gaattgacct gcggcaaaaa aaacgcaaac gttttccgaa cctgtttcag ggcagatttt
     1981 gctccttctt tgcactcaac ccgccgtaat catttatcgc ccgccaggcg acatgacacg
     2041 gtgcgattgc ctgacaccag cttgtctgac gctggctttt aggcggcaca actcccgcat
     2101 tacttaacgc tttaccattt acgctcaact gaatcaggtt aatgtagtaa ggcgttgggt
     2161 tagtgagttg tacaccctgc gaattccgtg tccagcgtaa ttgttgataa gcttccagcg
     2221 ggtcgcccgg caaaccttcg ggtcgccaga acagtttaaa taccgagcgc atcgccactt
     2281 ttacgctctg attttcaact ttgccggatg gcacgctggc aatgcttaac tcaaatagcg
     2341 tttcgcgatc cacaggcaag atgtcgcttt ccgttctcag caagcgcaac gtgcctgtag
     2401 taccgggctt caggagaata agtggcggag cggccagtaa tggtgctggc actgtcgacg
     2461 cttccccccc ggcccaacgc gttgggcggt tgattttact gttaataagc caggattcct
     2521 gactggtatt agtcagtaaa atagatatcg attctctgtc tgccggaaag ataaatcgcg
     2581 ttccgccgac caccactcct gcccgcaatt cacccgccac cagcagcgat aaaacggcga
     2641 aaatcaagag cgccaatctg ttccacgtat tagtcataat gaagtacaaa tgtcgcgctg
     2701 gcattcactt tacccgactg cacatcgcta ttgacaggtc gcaattgcgc ataaaaagtt
     2761 aatggcacct taccgctatg taggctgtag tctttactcg cctggttaag cggaatcaat
     2821 atcttcgcat catccagaat cgcaattgcc agcccgctgg ctgcatccgt accactttca
     2881 atggacagca tttcgctctg cactccgtcg ggggttcctg aaaaggtgac gtcaaccgtg
     2941 ctgacatctg ttccgcaatc ctgcaaatta atgatgaaag atttctccgg gccataattc
     3001 ccatgaaaaa aacgtgtcag cgagacatcg cctaactgca cattgatttg cttgctatcc
     3061 tgtgccagca cgcatgtatt acggcgaaac gatccgctaa tgttgactgt gccatcatgg
     3121 gcaaatagct cacagccccc cgttaatccc agtaagaaca gaatccagat tatccgtttc
     3181 aacatcactc cttcactggt aaaccagatc aaaatagaga accgccgtcg cattaccgcc
     3241 cgttgctccc gcctttgtgg acttataacg aagttgatat ttgagtgtgt tatccccggc
     3301 ttttaagggc gtaagaggct ggacctgatt gagcgggatt tcttgctgac tttgcgcatc
     3361 aagaatttgc acggcaatcc ccgttgccat accaccgctt ccgcctgtgt cagttagttt
     3421 gagcagtgtc ggcgcaaggg tgtcagcctc gccgctaaaa aggacgtccg cgccagtaat
     3481 accagcggca caaccggtga tatcgatatt caaatcagca gcagtgctta cggtattcgc
     3541 ggcctgaaaa tcactgatat taaaatcacc aatatgaata ttttgtaatg cacttttggt
     3601 ggcgacatca cagccctgat cgaccacatt cccataaata tgcatactaa cttgttcagg
     3661 cccagccatc ccgacatgga tgttaagaat gctaaagaac aagataacgc actgaaatat
     3721 cgtttttttc ttcatacgtt cacgcgttaa tcaaaattta ttctcaccgt tgccgtggct
     3781 tcaaatcgcc cggtttctgg tggtacacct gtcgtgctga ctggatagac attcattcgc
     3841 gcgatactgt ctgcatcatc aggaatagag agcggtaatg ttccaccagc aggggaaata
     3901 atgttgttct ggctatcggt caccaccacg cccacgccag gacgtgacgt cttaatcgcc
     3961 tggggatagc tggggtctgt ggttgccgtc agactcaggt tgacgttaac taaccccgcg
     4021 tttcccgtgt tacacctgac tggaattgca agttctgccg ttctggcccc aagaggctta
     4081 tgccctacaa cacgaaagtt agccgcaaat acgtcgccca gatcgacctc gattgtttct
     4141 ccggcattga cggaacatga gccaagtgaa ttgatagtac cagacaggta atacactaaa
     4201 accggtgtac ctgtagtcgt gcaggaatca cctgcgggta tgttgtagca ttcaaataat
     4261 cgcgccagcg ccataggagg aatgacgacc gaaccaacga acggctgcaa tattttcagg
     4321 gaaaagctac cgctattacc gctagccccc tggccaccta aacgttgacc gccgggatcg
     4381 caaatatgcc atcgtgtatc tgcccctgtg cccacgtttt cgaaaggaac cgcagtagga
     4441 gaactgcctt taacctgcaa taaaactttg gcggcaagat agtcattaat tttgtaccag
     4501 ttgtcgccta aatagactaa cggtgaatcc gcggcgaatg cccatcggcc acttctgaca
     4561 ttgtctttat cgcaatcaca ggaaacataa taatcattcg attcgttcca ctgataaaat
     4621 ttgtctttaa ctactccagc cgtattttct tcttcctcca gggttttaac gccaaaattg
     4681 acgccaatat acggattccc ctgagtggta tgacactgcc cgctataagc agcagtttga
     4741 gcgcaccata gcattaagac ggcggcaaaa aatagtcgta gtaataacac gcatttttct
     4801 ccaaagatta tctgcatatg gcatccattc gcgtaatcgc agagttaatt tgttgttcgg
     4861 taagctggta actgatatga caaatttgat cgggagcttc gccccagcgt acagttatcg
     4921 ctccctgagc gggcaaaccg gacatataga gcgagccatc atcatcaata atgccgctat
     4981 tagtctgtat gccgtccagc gtcgctatcg cgccaaaacg cagcggtata ccctgctttg
     5041 atgttttcat taataccctt gctcccgcat gggcattgaa ctccgccaga accaacgccc
     5101 ctttggtggg aaccacgttg actactgcat tttcaagatc cacgttacgt tttaacgatg
     5161 ccgcatcaag tgcgattcga ttttcccgat aagaagtggc ataggggaca acggtatatc
     5221 cgcgccagtc agttttcagc ccggtgctgt tcgccacacg agtattctct gcgcctggtg
     5281 ctgcaatcag gacgttagtt tcccccagcg attgccccag ggtaatgccc tgtgaatggg
     5341 caactaaact gcctgaaaga gcgtagttca gttgttgttg actgccgtta tcgctgtagt
     5401 tgtagcccac tcgggcatct gcaaacgccc ctttgtagtc catgctggcg ctaccattag
     5461 ccgttttccc ctcgctgtta tatccctgtt gcacgctgta gctcaggtta ccgtcatcga
     5521 gtagtgtctc cgttaaaccc aggttttgtg taatgtgccc ttcgttatcg atactggtgt
     5581 tagaggtcat ccaggcgtaa ttttttttcc tggtatagct atcattactt ctggggagca
     5641 acagattgag cggcagggaa aaatttagtg caaacacctg atcagcatca ggttgaccac
     5701 gggacttact gtagttccag gaacccccca gcgagagatc tttaatttgc gtgttgtagc
     5761 caaactgtaa tagccgatcc tgttgatcgg tatgccagta agtttgctgg ctaccactta
     5821 aataaaacga accgtactcg cctaattgct gggagatatt gacctgcagt ttgccacgtt
     5881 tggtgtaaaa caaattgtaa taacgcgacc acatcggcgt atcttcatca tcaccgtcat
     5941 taaattcgta gccatccata tgtttataca tggtgtcgga aagggtgtag aaacccgacg
     6001 tcgaatagcg atagcccatc aattgaaaat tagtgcccaa ttggttgagc gttttggcat
     6061 acagaaaacg atatgactga cctttatgtt cgctttggtc tgccagcgta ctcttcgcct
     6121 gggtcacatc gaacgaaatt gctccgaaat cgccaaggtt aaatcccaga ccaaacatgg
     6181 cggcacggta atattcagca tattgtccac caccatacca tgtcgttccc cacggtccgc
     6241 cccactgcaa cgtggcctgg gcaaatttgc tctcctgctg ttcattacta ttggttctgt
     6301 atttcgccag tgtcaccgcg tatttgattc gcccctgacg ctggagtaat ggcacgctgg
     6361 aaaacggtac gctgtagcta ttgacgctgc cgtccgcttc tttgatttca actaacaaat
     6421 caccgctcga cgacgtggaa tagagatcac taatttcaaa agcaccaggc gatacataag
     6481 tttggtaaat ggtgtaacca ttttgcttaa tcgttatctg ggcattgctt ttggcgatac
     6541 cacgcaccac aggcgcaaac cctttcaggc tgtctggcag catgttgtca tctgacgcca
     6601 gctgcacacc acgaaagctg acactatcga aaaaatcgcc tgcagtataa tcatcaccta
     6661 cggtcagttc gccttttagt ggaataaccg cccgctgcaa atagctgctg agattcttcc
     6721 attctgcggt ttggccacta ctgcgactcc atgttgaatt gttgcgcaat ctccatgggc
     6781 ctaaattaac gccactgttt aaattcagaa aatagctgtc gccagaatca ctgtctgcgc
     6841 tgctatgaat actgttagcc ccgctaaacg aatatcccag taatagcgcg ttgatccctt
     6901 catcccaacg ttcagggggg acataaccac gggcattact tttgatggcg atttgcggta
     6961 ctgaaatttc cagacggagt ttattcacat taaacgttac gctggcatcc ggaataatct
     7021 ccgccaatgg cacacacgct tgtttgtcca gttgtttaaa agccggaaac gcatcagtat
     7081 taacacccat tgcatccagg ctttcagtcg tcaaacaggc ggcaatcccc ttctctggcg
     7141 ttatctcaac aaaattgacg ttgcgcgtat ctacaattgt ctgattcaga acaatatcga
     7201 cacgataaac ccctggcgtt attttttgtc ctttttcgaa acgagacaaa tcggccacca
     7261 ttgagggatc atctttgaaa aaagcaggat cgaaccaggt ttcagcttgt gcattgaacg
     7321 ctgccgacac aaacaccacc agcccaccaa taaacgatgg cactccacca gagcttaaca
     7381 ggctgtgttg tcgatgagtt ctatacatct cgctctctta actgaagacg gcttacgatt
     7441 tacagttgag ttgcggcgtt tccgccccgt agtcattgat ggttcgatac gttacgctgc
     7501 tatttttggc cccctttggc agagaaaatt ggtatttatc aaagggggaa atcatttgat
     7561 ttttaactac ttttccacct attttcaggt ctgtcagggt aatgaaataa ggtgttgggt
     7621 tactgaccgt aaccatgtca gctgacgaag tgcaacgcag tttcgccgga gcctcgccta
     7681 agggatacgc taaacctttt ggtcgccaga aaagtttgat gacgctttgt gtggcaatct
     7741 tcagggtatt tccctcttcc cgcttcgttg ttgaagggac tgcccgtacg ctagtgaaga
     7801 aaagggtttc tctgtctttc gccaacggcg caccaatgta cataatgcgt aacagattct
     7861 cattagccgg attcagcaca aatagcggtg gtgtaataat aaaatctgct gattttttat
     7921 tatctggggt catcacccat gactgaataa gataaggaac atccggactg gtgttacgta
     7981 cacccagtga aatttgctct tcaccttctg aataaacaag acgtgttgct gcaagtccaa
     8041 tcccgccttt acctgccgcc catgcggatg agcagctgag taatattgcc gttaaactta
     8101 cggtcacaat tccctttgtt atgcaagttt tcataaatag tcactcttaa aaaatatgaa
     8161 tatcccctcc agaacactag aggggtacta attaataaaa caattaatga gttctattaa
     8221 taatagttaa tatggaaatt tgttaccgca ttcacattac cagaagtcgc tttccccgta
     8281 gcgatataat caactttaaa ggtcactgtt tgctcagcag aatccgctaa ctgaatattc
     8341 gacgtttcgg taccgggagt taccgatgta gaaccatctg gcagatacat ttgtagagca
     8401 acattcgtcg ctgcatcggt ggtagcctgg ttggcaaatg ctgtaggttg cacgccgtca
     8461 gccgtaccac taaaggtaaa cgtcgcattt ttagttacag taacatcaca gccttctaag
     8521 gcaattggta catcagtttt aacgccagag actgctccat tagtttgcaa cttggtggcg
     8581 ctgacatttg gcaatgtgac tactgaatct ttcgtcgcag ctaccagggt acaagctgga
     8641 gcaataactt taccattaaa tgttactttg ccatccgtga tagcattatc atcagctgcc
     8701 ataacgctgg acgttgcaca taccacagtt ataaacgccg tcaatacact ttttttcatc
     8761 gtcatcatcc tgatctcttc gtaaatatga taatgccttt aaatgacatt agacttaata
     8821 ataacaataa ttgaccgcca tttaaatcaa gcatactaaa tccaataagt gacaaatctt
     8881 taatttatag atttcatgaa tatttaaata ttaactgaac gagtaattca ttgattcaac
     8941 atctaatgac tatccaacaa ataaatatgc gtgatatata aagccctttc ttttgtttat
     9001 tttcagaaaa agatacacaa aatttatatt tggcattttc ttgtctctcc gacagactga
     9061 ccgaattgtt atcaatgtta acaaaaaaag aacaattggt tataaggaga gagtatgcgt
     9121 gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct ccagctcctt gctggaatat
     9181 gcgcgggaaa aactaaatgg cctcgatgta gaggtttatc actggaatct gcaaaacttc
     9241 gccccggaag atctgcttta tgctcgtttc gatagtccgg cactcaagac cttcaccgaa
     9301 cagctgcaac aggctgatgg actgatagtc gctacacctg tgtataaagc cgcctattcc
     9361 ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg ctttgcaagg caaagtggtg
     9421 ctaccgctgg cgacgggcgg taccgtggcc catctgctgg cggtcgatta tgcccttaaa
     9481 ccagttttaa gcgcactgaa agctcaggag atcctgcacg gcgtgtttgc cgatgactca
     9541 caagtaattg attaccatca cagaccccag ttcacgccaa atctgcaaac ccgtcttgat
     9601 accgcgctag aaactttctg gcaggcattg caccgccgcg atgttcaggt tcctgacctt
     9661 ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa ctggcgctgg tgggattgct
     9721 tagcgtctct acgattgcgg ttgctgcaga atcctcgcct gaagcgttac gtataggcta
     9781 tcagaaaggc agtattggta tggtactggc aaaaagccac cagttactgg aaaaacgcta
     9841 tccgcaatca aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt tggaagcgtt
     9901 aaacgttggc agtattgatc tcggcagtac cggggatatt ccgccaatct ttgcccaggc
     9961 tgccggggct gatttggtat atgtgggcgt cgagccaccg aagcccaaag ctgaagtgat
    10021 tctggtggca gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc acaaagttgc
    10081 ctttcagaaa ggttccagtt cacacaacct tttactgcgt gcactgcgtc aggccggact
    10141 taagtttacc gatatccaac ccacttacct gacgcccgct gatacccgcg ccgcgttcca
    10201 gcaaggtaac gttgacgcct gggctatctg ggatccctac tactctgctg cattattaca
    10261 gggcggcgtg cgggtgctga aagacggcac cgatctcaat caaactggat cgttttatct
    10321 ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt cagggcgtac tcgcaacctt
    10381 tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt tactggcaaa
    10441 aacgatgggc ttaccggcac cggtgattgc ctcttactta gatcatcgcc ctcctaccac
    10501 catcaaaccg gttaacgccg aggttgccgc cttacagcag caaacggcag atctgtttta
    10561 tgaaaatcgt ctggtgccga aaaaagtcga tattcgccag cgcatctggc agcccactca
    10621 actggaagga aaacaattat gagtctgaat atgttctggt ttttaccgac ccatggtgac
    10681 gggcattatc ttggaacgga agaaggttca cgcccggttg atcacggcta tctgcaacaa
    10741 attgcgcaag cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg
    10801 tgtgaagatg cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg gctgaagttt
    10861 cttgtcgccc tgcgccccag cgtaacctca cctaccgttg ccgctcgcca ggccgccacg
    10921 cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcag cgatccacaa
    10981 gagctggcag gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa
    11041 tttacccagg tctggcggcg cttattgctg ggggaaaccg tcaatttcaa cggtaaacat
    11101 attcatgtgc gcggagcaaa actgctcttc ccgccgattc aacagcctta tcctccgctt
    11161 tactttggcg gatcgtcaga cgtcgcccag gagctggcgg cagaacaagt tgatctctac
    11221 ctcacctggg gcgaaccgcc agaactagta aaagagaaga tcgaacaagt gcgggcgaaa
    11281 gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa
    11341 actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact
    11401 atcgccaaag cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg
    11461 gcgttacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt
    11521 ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgctgcg
    11581 cgaatcaacg aatacgccgc gcttggcatc gacagctttg tgctttcggg ctatccgcat
    11641 ctggaagaag cgtatcgggt cggcgagttg ctgttcccac atctggatgt cgccattccg
    11701 gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc
    11761 ccccgtaaag tcgcgcaaag ctaaggaaga aataatggca acgccagtga agaagtggtt
    11821 attgcgcgtt gccccctggt ttttaccggt gggcatcgtg gcggtgtggc aactggcctc
    11881 ctcagttggc tggctgtcga cgcgtatttt gccctcaccg gaaggggtgg tgacggcgtt
    11941 ctggacgctc tccgccagcg gcgaactgtg gcagcatctg gcgatcagtt cctggcgggc
    12001 gctgattggc ttttcaattg gcggatcgct ggggttgatt ttgggactga ttagcgggct
    12061 atctcgctgg ggagaacggc tgctggatac ctcaattcag atgttgcgca acgtaccgca
    12121 tctggcgctg atcccgctgg tgattttgtg gtttggcatt gatgaatccg caaaaatctt
    12181 tctggtggcg ctcggcacgc tattccccat ttatatcaac acctggcatg gcatccgtaa
    12241 tatcgatcgc ggtctggtgg aaatggcgcg tagctatgga ttatccggca taccgctgtt
    12301 tatccatgtg atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc gttttgcgtt
    12361 gggcctgatg tggctgacgc tgattgttgc cgaaaccatt tctgccaatt caggcattgg
    12421 ttatctggcg atgaatgcgc gggagttttt gcaaacggac gtggtggtag tcgctattat
    12481 tctttacgcc ctgctcggca aacttgctga cgtcagcgcg cagctgttag agcgcctctg
    12541 gttgcgctgg aacccggctt atcatttgaa ggaggccacg gtatgaatac tgctcgtctg
    12601 aaccagggca caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc
    12661 ctgaaccaac tggatttaca tattccggca ggtcagtttg tggcggtggt gggccgcagc
    12721 ggtggtggca aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc aaccgcaggc
    12781 gatgtgttag cgggcaccac accgctggct gagattcagg aagacacgcg aatgatgttt
    12841 caggatgcgc gtctgctgcc gtggaaatcg gtgattgata acgttgggtt aggccttaaa
    12901 ggccagtggc gcgatgccgc gcgtcgggcg ttggctgcag taggactgga gaatcgcgcc
    12961 ggggaatggc ctgccgcact ttctggcggg cagaaacagc gtgtggcgct ggcaagagcg
    13021 ttgattcatc gaccgggttt attgttgctt gatgaaccgc tcggggcgct ggacgcctta
    13081 acgcgactcg agatgcagga tttgattgtg tcactttggc agcagcacgg ctttaccgta
    13141 ctgttggtga cgcatgatgt gagcgaagcg gtagcaatgg ctgaccgggt gttgttaatt
    13201 gaagagggaa aaattggtct ggatttgaca gtggatatcc cccgtccgcg tcgattaggg
    13261 tcggtgcgat tagcggagct ggaagcggaa gtattgcaac gggtaatgcg gcgtgggcat
    13321 tctgaacaac tgattcggcg tcatggttaa tatgccggat gtggcgcaca aaaccagcat
    13381 tttcaacgga ctagtaggcc tgataagaca cgtcaagcat cgcatcaggc agttgggcac
    13441 ggctgccgga tgcggcgtga acgccttatc cagcctacaa aaccagcaat ttcaacggag
    13501 ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca ttttggctca aaagtatcaa
    13561 gccagtgctt tggtgatctt ctcgtacaga tcgccagaga gattttccag ccctttcaac
    13621 tgttccagcg ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc
    13681 agcggttcaa tcagacgtga agctacctgc gggttacggc tgttgaggtc ggtaagcatt
    13741 tccaccagga actggtaacc gctgccatct tcggcatgga acgctgccgg gttgctgccc
    13801 gcaaacgcac caatcaacga acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc
    13861 tgcaacagac cacgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata
    13921 aaccatttat ccatcaccag accgtcctga tgccacttat cgtcgtactc ctgcatcagc
    13981 gcgtcacggc aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc
    14041 atattgtttg cttcatggta ttgcttgctc accagcacgt cagccagatg cgtttcacca
    14101 aaagcaagga agcgcaggca ggcattacgc agagtgcgtt ttgcaatatc ttcatgctca
    14161 acacggtact cgctctggta attcgcgttg taaatagcca gcagctcatc cgccagttca
    14221 gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata
    14281 tcgaacagtt cagccatttc attgacagaa ggcagcgtca ggatttctgc cgccagcgcc
    14341 ggatcaatct tctcatcgag cagcaccgcg cggaaagcgt cagccacatg taccggcaga
    14401 gacagcggct gcccttgctg atgacgggcg acgttcagct tgatgtaggt tgccagcaaa
    14461 ctttgcgccg catcccaacg ggagaaatca ttacgcgcat gacgcatcag gaaggtcagt
    14521 tgctgatcgc tccacttata ttccagtttc actggcgcag agaattcgca cagcagcgca
    14581 ggcaccggct ggaagtagac attatcaaaa acaaaggtct gttccgcctg ggtgacgttc
    14641 agcacggaat tcaccggatg accgcctttc tgcaacggga tcactttgcc ttcgttgtca
    14701 tacaattcga tggcaaatgg aatatgcagc ggctgttttt ctgcctgatc cggcgtggct
    14761 ggcgtgcgct ggctgatggt cagggtgtac tgctcggttt ccggattgta gtcgtctttg
    14821 acggtcacaa tcggcgtgcc ggactggctg taccaacggc ggaaatggga gagatcgaca
    14881 ttcgacgcat cttccatcgc ctgcacaaag tcgtcgcagg ttgccgcgct gccatcatgg
    14941 cgctcgaaat atagttgcat ccctttctgg aagttttctt cgcccagcag ggtgtggatc
    15001 atgcgaatca cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt gttcatctca
    15061 atgaccatat ccgggcggat cgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat
    15121 ccgcgcatgg tgcgcacatt attgatacgg tttaccgcgc gggaaccaag gtcagagctg
    15181 aattcctgat cgcggaagac ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg
    15241 caggtcactc ggttaccggt ccagttatgg aaatattcat ggccgataac gcgttcaata
    15301 tcgagataat ctttgtcggt ggcggtgtcg gtgcgggcca acacatattt ggagttaaag
    15361 atattcagac ccttattctc cattgcgccc atattgaaga aatccaccgc gacgatcata
    15421 tagatgtcga ggtcatactc caggccaaag cgttcttcat cccatttcat ggagtttttc
    15481 agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta cagctccagt
    15541 gctacttcgc gaccagaacg cgtggtaaag gtatcgcgca gtacatcaaa gtcgcctgcc
    15601 accagcgcaa acaggtagca cggtttcggg aacgggtcct gccactgtac ccaatggcgt
    15661 ccgttttcca gttcgccttg cgcaacgcgg ttaccattgg aaagcaggaa gggatatttg
    15721 attttatcgg caataatttt ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa
    15781 tacgtaatat ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc gcctgactga
    15841 taaagccctt ccagcgccgt attcgccgcc gggcttattt cattaatgat cttaagcgta
    15901 aaacgctccg gcaaattact gatgaccagt gcgccctctt cttctttcca ggcggtccac
    15961 ggctcatcat taatatgaac agaaaccagt ttgaggtctt cgccgttgag acgaagcgga
    16021 gcatctgatg caccatgacg gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg
    16081 tcgaggtcaa aggtcaagtc aatatcagta atctggtaat ccggcgcacg ataatcgtga
    16141 cggtatttgg cttgtggctg ttgagtcata aataaccttt agcatctttt atagagtctg
    16201 gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta
    16261 caaacgcctt tattgctaca tttttataac atacaccgcg taatgccatc gaccagaaag
    16321 gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac aaggtatact ccagcagttc
    16381 ctgaagatgt ttattgtact aaacgctcct gtacgaggac gctactgcgc acctatgaca
    16441 caattcgctt ctcctgttct gcactcgttg ctggatacag atgcttataa gttgcatatg
    16501 cagcaagccg tgtttcatca ctattacgat gtgcatgtcg cggcggagtt tcgttgccga
    16561 ggtgacgatc tgctgggtat ttatgccgat gctattcgtg aacagattca ggcgatgcag
    16621 cacctgcgcc tgcaggatga tgaatatcag tggctctctg ctctgccttt ctttaaggcc
    16681 gactatctta actggttacg cgagttccgc tttaacccgg aacaagtcac cgtatccaac
    16741 gataatggca agctggatat tcgtttaagc ggcccgtggc gtgaagtcat cctctgggaa
    16801 gttcctttgc tggcggttat cagtgaaatg gtacatcgct atcgctcacc gcaggccgac
    16861 gttgcgcaag ccctcgacac gctggaaagc aaattagtcg acttctcggc gttaaccgcc
    16921 ggccttgata tgtcgcgctt ccatcttatg gattttggca cccgtcgccg tttttctcgc
    16981 gaagtacaag aaaccatcgt taagcgtctg caacaggaat cctggttcgt gggcaccagc
    17041 aactacgatc tggcgcgtcg gctttccctc acgccgatgg gaacacaggc acacgaatgg
    17101 ttccaggcac atcagcaaat cagcccggat ctagccaaca gccagcgagc tgcacttgct
    17161 gcctggctgg aagaatatcc cgaccaactt ggcattgcat taaccgactg catcactatg
    17221 gatgctttcc tgcgtgattt cggtgtcgag ttcgccagtc gctatcaggg cctgcgtcat
    17281 gactctggcg acccggttga atggggtgaa aaagccattg tacattatga aaagctggga
    17341 attgatccac agagtaaaac gctggttttc tctgacaatc tggatttacg caaagcggtt
    17401 gagctatacc gccacttctc ttcccgcgtg caattaagtt ttggtattgg gactcgcctg
    17461 acctgcgata tcccccaggt aaaacccctg aatattgtga ttaagttggt tgagtgtaac
    17521 ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa ctatctgcca cgataaagcg
    17581 tttgttcggg cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc cagttaatat
    17641 catcagggag ctaatcggct cccttttttt acctttaatt ccgaaatctt tcgctgcatt
    17701 tgcgaattct gcttgtctga ttgcagatgc caggtaacat aggtatcccc ccatttacgg
    17761 gtggacaagt gtttattttt tccgactatt aacagagaga atattatgag cgttgtgcct
    17821 gtagccgacg tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg
    17881 gtacgtaccc gccgagattc aaaagctggc atctccttcc tcgccgttta tgacggttcc
    17941 tgctttgatc ctgtacaggc tgtcatcaat aattctctgc ccaattacaa tgaagacgtc
    18001 ctgcgtctga ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc gtcgccgggc
    18061 caggggcaac aatttgaaat tcaggccagc aaagttgaag ttgctggttg ggttgaagat
    18121 ccagacactt acccgatggc ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct
    18181 cacctgcgtc cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca tacgctggcg
    18241 caggcgctgc atcgcttctt taacgagcag ggattcttct gggtttcaac gccactgatt
    18301 accgcatctg ataccgaagg tgcaggcgaa atgttccgcg tttctacgct ggatctggaa
    18361 aacctgccgc gtaacgatca gggcaaagtg gatttcgaca aagacttctt tggtaaagag
    18421 tctttcctga ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc attatccaaa
    18481 atttatacct tcggcccgac tttccgtgct gaaaactcca acaccagccg tcacctggcg
    18541 gaattctgga tgctggagcc ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg
    18601 gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc agacgacatg
    18661 aaattcttcg ctgaacgcgt agataaagat gccgtttcac gtctggaacg cttcattgag
    18721 gccgatttcg cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa ctgcggcagg
    18781 aagtttgaaa acccggttta ctggggcgtc gatctctctt ctgagcatga gcgttatctg
    18841 gcggaagaac actttaaagc accggtagtg gttaaaaact atccgaaaga tattaaagcg
    18901 ttctatatgc gcctcaacga agacggtaaa accgttgcgg ctatggacgt tctggctccg
    18961 ggcatcggtg agatcattgg tggctcccag cgtgaagaac gtctggacgt tctggacgag
    19021 cgtatgctgg aaatgggcct gaataaagaa gattactggt ggtatcgcga tctgcgtcgc
    19081 tacggtactg ttccgcattc aggtttcggt cttggttttg aacgcctgat tgcttacgta
    19141 actggcgtgc aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg caacgccagc
    19201 ttctgatttt cttcctcaaa gagccagcgc ctgctggctc ttttttatcc tttctgtcaa
    19261 gttatctgtt tgttaagtca agcaatctat ttgcaacccc gccataacga tcatcctgtt
    19321 acggaatatt acattgcaac atttacgcac aaaaaataac ccgcattctt attgcggatt
    19381 agttttttct tagctgatag cacaattttc atactatttt ttggcattct ggatgtctga
    19441 aagaggattt tgtgccaggt cgataaagtt tccatcagaa acaaaatttc cgtttagtta
    19501 atttaaatat aagtaaatca tataaataga taaaaattgc tgtaaatatc atcacgtttc
    19561 tatggaaata tgacggtgtt cacaaagttc cttaattttt acttttggtt acatattttt
    19621 tctttttgaa actaaatctt tatctttgta gcactttcac ggtagcgaaa cgttagtttg
    19681 aatggaaaga tgcctgcaga cacataaaga caccaaactc tcatcaatag ttccgtaaat
    19741 ttttattgac agaacttatt gacggcagtg gcaggtgtca taaaaaaaac catgagggta
    19801 ataaataatg atgaagcgca atattctggc agtgatcgtc cctgctctgt tagtagcagg
    19861 tactgcaaac gctgcagaaa tctataacaa agatggcaac aaagtagatc tgtacggtaa
    19921 agctgttggt ctgcattatt tttccaaggg taacggtgaa aacagttacg gtggcaatgg
    19981 cgacatgacc tatgcccgtc ttggttttaa aggggaaact caaatcaatt ccgatctgac
    20041 cggttatggt cagtgggaat ataacttcca gggtaacaac tctgaaggcg ctgacgctca
    20101 aactggtaac aaaacgcgtc tggcattcgc gggtcttaaa tacgctgacg ttggttcttt
    20161 cgattacggc cgtaactacg gtgtggttta tgatgcactg ggttacaccg atatgctgcc
    20221 agaatttggt ggtgatactg catacagcga tgacttcttc gttggtcgtg ttggcggcgt
    20281 tgctacctat cgtaactcca acttctttgg tctggttgat ggcctgaact tcgctgttca
    20341 gtacctgggt aaaaacgagc gtgacactgc acgccgttct aacggcgacg gtgttggcgg
    20401 ttctatcagc tacgaatacg aaggctttgg tatcgttggt gcttatggtg cagctgaccg
    20461 taccaacctg caagaagctc aacctcttgg caacggtaaa aaagctgaac agtgggctac
    20521 tggtctgaag tacgacgcga acaacatcta cctggcagcg aactacggtg aaacccgtaa
    20581 cgctacgccg atcactaata aatttacaaa caccagcggc ttcgccaaca aaacgcaaga
    20641 cgttctgtta gttgcgcaat accagttcga tttcggtctg cgtccgtcca tcgcttacac
    20701 caaatctaaa gcgaaagacg tagaaggtat cggtgatgtt gatctggtga actactttga
    20761 agtgggcgca acctactact tcaacaaaaa catgtccacc tatgttgact acatcatcaa
    20821 ccagatcgat tctgacaaca aactgggcgt aggttcagac gacaccgttg ctgtgggtat
    20881 cgtttaccag ttctaatagc acacctcttt gttaaatgcc gaaaaaacag gactttggtc
    20941 ctgttttttt ataccttcca gagcaatctc acgtcttgca aaaacagcct gcgttttcat
    21001 cagtaatagt tggaattttg taaatctccc gttaccctga tagcggactt cccttctgta
    21061 accataatgg aacctcgtca tgtttgagaa cattaccgcc gctcctgccg acccgattct
    21121 gggcctggcc gatctgtttc gtgccgatga acgtcccggc aaaattaacc tcgggattgg
    21181 tgtctataaa gatgagacgg gcaaaacccc ggtactgacc agcgtgaaaa aggctgaaca
    21241 gtatctgctc gaaaatgaaa ccaccaaaaa ttacctcggc attgacggca tccctgaatt
    21301 tggtcgctgc actcaggaac tgctgtttgg taaaggtagc gccctgatca atgacaaacg
    21361 tgctcgcacg gcacagactc cggggggcac tggcgcacta cgcgtagctg ccgatttcct
    21421 ggcaaaaaat accagcgtta agcgagtgtg ggtgagcaac ccaagctggc cgaaccataa
    21481 gagcgtcttt aactctgcag gtctggaagt tcgtgaatac gcttattatg atgcggaaaa
    21541 tcacactctt gacttcgatg cactgattaa cagcctgaat gaagctcagg ctggcgacgt
    21601 agtgctgttc catggctgct gccataaccc aaccggtatc gaccctacgc tggaacaatg
    21661 gcagacactg gcacaactct ccgttgagaa aggctggtta ccgctgtttg acttcgctta
    21721 ccagggtttt gcccgtggtc tggaagaaga tgctgaagga ctgcgcgctt tcgcggctat
    21781 gcataaagag ctgattgttg ccagttccta ctctaaaaac tttggcctgt acaacgagcg
    21841 tgttggcgct tgtactctgg ttgctgccga cagtgaaacc gttgatcgcg cattcagcca
    21901 aatgaaagcg gcgattcgcg ctaactactc taacccacca gcacacggcg cttctgttgt
    21961 tgccaccatc ctgagcaacg atgcgttacg tgcgatttgg gaacaagagc tgactgatat
    22021 gcgccagcgt attcagcgta tgcgtcagtt gttcgtcaat acgctgcagg aaaaaggcgc
    22081 aaaccgcgac ttcagcttta tcatcaaaca gaacggcatg ttctccttca gtggcctgac
    22141 aaaagaacaa gtgctgcgtc tgcgcgaaga gtttggcgta tatgcggttg cttctggtcg
    22201 cgtaaatgtg gccgggatga caccagataa catggctccg ctgtgcgaag cgattgtggc
    22261 agtgctgtaa gcattaaaaa caatgaagcc cgctgaaaag cgggctgaga ctgatgacaa
    22321 acgcaaca
//
LOCUS       DAHBHC010000081        22155 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHBHC010000081 DAHBHC010000000
VERSION     DAHBHC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.081"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..156
                     /locus_tag="M2A35_003818"
     CDS             <1..156
                     /locus_tag="M2A35_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCB7200062.1"
                     /translation="TIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVIWQKL
                     IPSQADS"
     gene            229..480
                     /locus_tag="M2A35_003819"
     CDS             229..480
                     /locus_tag="M2A35_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCB7200063.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            515..1795
                     /locus_tag="M2A35_003820"
     CDS             515..1795
                     /locus_tag="M2A35_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7200064.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVTDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFIFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(1815..1925)
                     /locus_tag="M2A35_003821"
     CDS             complement(1815..1925)
                     /locus_tag="M2A35_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCB7200065.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(1983..3002)
                     /locus_tag="M2A35_003822"
     CDS             complement(1983..3002)
                     /locus_tag="M2A35_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HCB7200066.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3014..4228)
                     /gene="rspA"
                     /locus_tag="M2A35_003823"
     CDS             complement(3014..4228)
                     /gene="rspA"
                     /locus_tag="M2A35_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCB7200067.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4434..4760)
                     /locus_tag="M2A35_003824"
     CDS             complement(4434..4760)
                     /locus_tag="M2A35_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCB7200068.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4895..5236
                     /locus_tag="M2A35_003825"
     CDS             4895..5236
                     /locus_tag="M2A35_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCB7200069.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5271..5831
                     /gene="speG"
                     /locus_tag="M2A35_003826"
     CDS             5271..5831
                     /gene="speG"
                     /locus_tag="M2A35_003826"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCB7200070.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5834..6544)
                     /locus_tag="M2A35_003827"
     CDS             complement(5834..6544)
                     /locus_tag="M2A35_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCB7200071.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VNPAVERVYTIKNTIDYY"
     gene            6652..6957
                     /locus_tag="M2A35_003828"
     CDS             6652..6957
                     /locus_tag="M2A35_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCB7200072.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7156..9582
                     /locus_tag="M2A35_003829"
     CDS             7156..9582
                     /locus_tag="M2A35_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCB7200073.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQRSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9643..12066
                     /locus_tag="M2A35_003830"
     CDS             9643..12066
                     /locus_tag="M2A35_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCB7200074.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12077..12694
                     /gene="dmsB"
                     /locus_tag="M2A35_003831"
     CDS             12077..12694
                     /gene="dmsB"
                     /locus_tag="M2A35_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCB7200075.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGSEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12696..13550
                     /locus_tag="M2A35_003832"
     CDS             12696..13550
                     /locus_tag="M2A35_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCB7200076.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13593..14207
                     /gene="dmsD"
                     /locus_tag="M2A35_003833"
     CDS             13593..14207
                     /gene="dmsD"
                     /locus_tag="M2A35_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCB7200077.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14366..15658
                     /gene="clcB"
                     /locus_tag="M2A35_003834"
     CDS             14366..15658
                     /gene="clcB"
                     /locus_tag="M2A35_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCB7200078.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAASYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15611..16306)
                     /gene="bioD"
                     /locus_tag="M2A35_003835"
     CDS             complement(15611..16306)
                     /gene="bioD"
                     /locus_tag="M2A35_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCB7200079.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16431..17651)
                     /gene="mlc"
                     /locus_tag="M2A35_003836"
                     /gene_synonym="dgsA"
     CDS             complement(16431..17651)
                     /gene="mlc"
                     /locus_tag="M2A35_003836"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCB7200080.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17786..18679)
                     /locus_tag="M2A35_003837"
     CDS             complement(17786..18679)
                     /locus_tag="M2A35_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7200081.1"
                     /translation="MNIELRHLRYFVAIAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYEDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18786..20039
                     /locus_tag="M2A35_003838"
     CDS             18786..20039
                     /locus_tag="M2A35_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7200082.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            20436..20771
                     /locus_tag="M2A35_003839"
     CDS             20436..20771
                     /locus_tag="M2A35_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCB7200083.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETANTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            20864..20947
                     /locus_tag="M2A35_003840"
     CDS             20864..20947
                     /locus_tag="M2A35_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200084.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            21047..21868
                     /locus_tag="M2A35_003841"
     CDS             21047..21868
                     /locus_tag="M2A35_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCB7200085.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDCWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21907..>22155)
                     /gene="mdtI"
                     /locus_tag="M2A35_003842"
     CDS             complement(21907..>22155)
                     /gene="mdtI"
                     /locus_tag="M2A35_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCB7200086.1"
                     /translation="FSDGFRRKIFGLLSLAAVLAAFSALSQAVKGIDLSVAYALWGGF
                     GIAATLAAGWILFGQRLNRKGWIGLVLLLAGMIMVKLA"
BASE COUNT         5676 a         5559 c         5384 g         5536 t
ORIGIN      
        1 acaatcgttg agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa
       61 ggtgagcgcc ataatgcact tgatgacgcc cgttaccagg caaaatacgt ttcagttatc
      121 tggcaaaaac tgatcccgag tcaggctgat tcttaatgtt caactgtcgc cggttgtgac
      181 tggtattctg caaccggcgc tcgtctgatg taagagataa agaaatcgat gagcgaagta
      241 atcatgattg tctctcccgg caaatgggta tccgaagagc agttaattgc gctgaaagga
      301 ataaaaaaag gtacgttaaa aaaggcccgg gaaaaatcgt ttatggaagg aagggaatat
      361 aagcatgtcg ctcatgacgg tatgccatgg gataacagtc catgctttta caacctggaa
      421 gaaattgatc gctggattga gcgccaggca tcagcaagac caagacgtca tcttacttga
      481 ctaaaaacca cactaattaa tgagagaagt tgaaatgaaa tatccgacag gcgtggaaaa
      541 ccatggaggg aaattacgta tctggtttgt ttataaaggc gtaagagtca gggaaaatct
      601 gggggttact gacacagcaa aaaacaggcg cgttgcaggt gaactacgcg cctctgtttg
      661 ttacgcaata aaaactggcg ttttcgacta tgcaaaacag tttccctcct cacgcaatct
      721 ggaaaaattt ggtgaggccc gacaagattt aaccataaaa gaactggctg aaaaattcct
      781 ggcactgaaa gaaactgaag tcgcaaaaac atcactcaac acataccgtg ccgtcatcaa
      841 aaatatcctg agcataatcg gtgaaaaaaa tcttgcctca tcgattaata aagaaaaatt
      901 actggaggtt cgtaaagagc tactgactgg ataccagatc cccaaaagta actatattgt
      961 tacacaacca gggagatcgg ctgtaactgt aaataattac atgacaaatc ttaacgccgt
     1021 gttccagttt ggtgttgata acggttacct ggcagataac ccgtttaagg ggatctcgcc
     1081 attaaaggaa tcaagaacca ttccggatcc tctttcgcgg gaggaattca tccgtcttat
     1141 cgatgcgtgc agaaatcagc aagccaaaaa tttatggtgt gtttctgttt atactggcgt
     1201 tcgccctggt gagctgtgtg cacttggatg ggaggacata gatctgaaaa atggaacaat
     1261 gatgatcagg agaaatttag caaaagaccg tttcacggta ccaaaaacac aggcgggaac
     1321 caatcgggtc attcatctta ttaagccagc aatcgacgct ctccggagtc agatgacatt
     1381 aacgagactg agcaaagagc atatcattga tgttcacctc agagagtatg gcagaacaga
     1441 aaaacaaaaa tgcaccttta tttttcaacc tgaagtgtca gcgagagtaa aaaattatgg
     1501 tgaccatttt accgttgact caataaggca gatgtgggac gcagcgataa aacgtgccgg
     1561 actccgccat cgaaaatcat atcagtcgag acatacttat gcctgctggt cgctgacagc
     1621 tggtgctaac ccggcattta tagcaaacca gatgggccat gcagatgcgc aaatggtatt
     1681 tcaggtatac ggaaaatgga tgtctgaaaa caataatgca caggtagctt tgttaaatac
     1741 acagttaagc gagtttgccc caaccatgcc ccataacgaa gcaatgaaaa attaatttaa
     1801 tatttatcaa atggttaaca cgcatgactc ttgaaatcca taaattcaag cgcagtgccc
     1861 agccatcccg atactgctgc tttcaccaaa tccttagtgc ttctttcgtg tttttctatt
     1921 gtcataatgg ttatctctaa aaaagaggta agatgcgtac tacttactcg ccgttattgg
     1981 tattattcag aaaaagtgag taagactttg cagcaatgtt tttgatcctg ttcaaataaa
     2041 ctaatggcat cagcaacatg ctggaaatca aacgtatggg taattaattt ttctggttta
     2101 attaaccctt tacttaacca gtcgataacg accgggaatt tatttgcatt taagcgtgaa
     2161 gaaaaaatag agagttcttt tccggtaatt ccttgctgaa tcacttcaga cggttcactg
     2221 gagaagccca tcaatacaat acgtgccgct ggagaagcca gcgttacggc ctctttcagg
     2281 atagaaggat gacaagccgc atcgataatt aatgtcggct tgatgccttt ttcagtgaaa
     2341 atctcgccaa gcggtgtctg gctgttatta atcgcccagt cagccccgct ctctttcgct
     2401 ttttccagtc gttcatcaat gcgatcggca acaatcacat ttttaacgtt atagacgcct
     2461 tttaatacct gaacgatcgt caggccgatt ggaccggcac cataaaccag aacggtatca
     2521 ttttcagtcg gttgaccatg tccggttacg ttagccgcaa tggtaaaagg ttcgatcatt
     2581 accgcatatt gatcggccac tgcttcagga attttccacg cattttttgc cggaaccacg
     2641 gcatattcac tgaaaccacc gtcagcgtgc acacctaata cagccagtgt cgtacaaacg
     2701 ttcggtttac ctatagagca cggatagcaa tgcccacagc tgaccaccgg atcgacagca
     2761 acacgttcac cgactctggc gctttccacg ccttcaccca ctgcatcaat gacgccaaag
     2821 aattcatgac caatgacgcg cggatatttc gcaaaaggat tatgcccacg ataaatatgg
     2881 ctatctgaac cacaaattcc ggcaagtttc acttttactc gcacttcacc cgctgacggg
     2941 gtgggtattt cacgttcgac aatcgccagt tgattcggtt tttcaattaa tatgcttttc
     3001 attatcttac tccttaccag ttccacagcg tgccatcttc cagacgtgcg actggtagat
     3061 aagcaggttc atagggatat ttcgccgcca gcttttcatc gaattcgata ccaagacccg
     3121 gtttgtctcc cggatgcata tagccgttat cgaaagtcca gttgtgcggg aagacttcga
     3181 gcatttgttc ggaataaccc atgtattcct ggacaccgaa attggggacc cacagatcaa
     3241 agtgcagcgc cgcagccatg cagactggtg acaaatcgga aggaccgtgt gagccagtac
     3301 gtacctgata cagcgaagca aaatcggcaa tccggcgcat accggtaatt ccgcctgcat
     3361 gggtcagcgt ggtgcggata taatcgatga gttgctcttc aatcagttgt ttgcagtccc
     3421 agatgctgtt gaagacttca cccactgcga tgggtgtgac ggtatgttgg cgaatgagac
     3481 ggaagcattc ctggttttcc gcaggtgtcg ggtcttccat ccagaacatg cgataatctt
     3541 caatgctttt accaaagcgc gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca
     3601 gcaaatgttc attaaaacca aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat
     3661 cgaggtattt ctccgtcgac cacagctgct cttccggcca ctgtcctttg gttgcgggtt
     3721 cataagccag acctttacct ttcgacatgc cgtaggtggt tttcatacca gggattccgc
     3781 actgcacgcg gatggctttg aatcccagct cctgatgacg ggcataatca tccagagctt
     3841 catcaatact gtgaccggtg gtatggcaat aaaccatcac cccttcacga gacgcgccgc
     3901 cgagtaactg gtaaagcggc atgttggccg ctttggcttt aatatcccac agcgccatat
     3961 caaccgctga aatggccgac atcgtaaccg gaccgcgacg ccagtaagca cctttataga
     4021 aaaactgcca gatatcttcg atacggtgcg catcgcgacc aataagctgc ggacaaaggt
     4081 gatcctgcaa ataagaggcc acggaaagct cacgtccatt gagagtggca tccccaaggc
     4141 ccgtaatacc gtcctcagtg gtgattttta atgtgacgaa attacgcccc ggacaggtaa
     4201 caaaaacttc agcctttacg atcttcatgt tcgattcctt gcatcgcttg tcgtgatgca
     4261 tgaaatctac gcaactgagc tactaccata caagtatgag gatcgaaaaa agcctgagtg
     4321 atcacaaaaa aaggcgtata tttgcgctgt gaatggttga caaaagatga aatagaatac
     4381 cttttgtcag ctgacacttc ctcgtatctt attgataaaa tggatttatg ttcctacgtg
     4441 cgcccccagc ccgcaacaat gatcaacatg ccgcaaagcg caatcaacgc acccgtccag
     4501 tcataaagag tcagtttcac gccatccaca acgcgcagcc acatcaacgc cgtgcagaca
     4561 taaacgccac cataagccgc gtaaacacgc ccactcgccg caggatgcaa cgttaacaac
     4621 cagacaaaca gcgccagtga aatccccgcc ggaagcaaca gccagatact ggcgtttcgt
     4681 tttaaccaca accagggcag aaagcatcca ataatttcac acagcgcagt agcaaaaaat
     4741 agtaacgttg ttttaatcat ctttgtcact tattgacatc atgtatagtt atagggccac
     4801 ataatatcac caatataaac accctcctga tacgttttgc gttcgcagtg gatggtgtag
     4861 aatcaccttt aatcattcat acagggaatg aattatgaaa atcactctca gcaaacgaat
     4921 cggcctgctc gcttttctgc taccttgcgc actggcattg agcacaactg ttcatgccga
     4981 aactaacaaa ctggtgattg agtctggcga cagtgcacaa agccgccagc acgccgctat
     5041 ggaaaaagag caatggaatg acacgcgcaa tctgcgccag aaagtgaata aacgcactga
     5101 aaaagagtgg gataaagccg acgccgcttt tgataaccgc gataaatgtg agcaaagcgc
     5161 caacatcaat gcctactggg agccaaatac tttgcgctgc ctggaccgtc gaactggccg
     5221 cgttattacc ccctaacctg ttattgattt aaggaatgta aggacacgtt atgccaagcg
     5281 cccacagtgt taagctacgc ccgctggagc gtgaagattt acgctatgta catcaactcg
     5341 acaataacgc cagtgtgatg cgttactggt ttgaggaacc ctacgaagcc tttgttgaac
     5401 tctctgatct gtatgataag catattcacg atcagagcga acggcgcttt gtggtggaat
     5461 gtgacggcga aaaagccggt ctggtggagc tggtggaaat taaccatgtt catcgccgcg
     5521 cagaatttca gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg
     5581 caaaattagc aatggactat ggctttaccg ttctcaatct ctataagctg tatctgatcg
     5641 ttgataaaga gaatgaaaaa gcgattcata tttaccgcaa gcttggcttt tcggttgaag
     5701 gtgaattgat gcacgagttc tttattaatg gtcaatatcg taatgccatt cgcatgtgta
     5761 tattccagca tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga
     5821 ccgcacaata gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg
     5881 cggggtttac tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt
     5941 catattcaca gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaactgctg
     6001 tataatctaa ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt
     6061 cgttactttt cgtggtttta cccagcggat acccctgatc atcatagcga tattccatct
     6121 gcatttgttt gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc
     6181 cggcagaagg taattctgct agctggcatt ttccctgtaa acgtactctt ttctccagcg
     6241 tctcagcatc acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca
     6301 gatccagtaa ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt
     6361 tcgtcacttc accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca
     6421 gcggatcaaa atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta
     6481 cctctgtgcg gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt
     6541 tcacaacagt caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt
     6601 acacgttaaa tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc
     6661 tcaacctgct gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg
     6721 ggatcctgtg agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc
     6781 gaaagcagct tcacgttaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag
     6841 gtcgtcgggc attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcggt
     6901 aacgtctctg ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaacac
     6961 attatcgact gaacgccgga tacgacacat ccggcgattt gaacatacaa cataatccca
     7021 ccttattact catacccttc tattgatatg gattaataat tcttaaccca aaatgggtag
     7081 actccctcta ttgttagcgc gctaaatatt caatatataa acttttatat aacgataaag
     7141 aacagggagt gagttatgtc caaaaatgaa cgaatggtgg gcatcagccg cagaacactc
     7201 gttaaatcta ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttgccgttt
     7261 accctgcgca gtgcagcagc agcggtacaa caggcccgcg aaaaagtggt ctggggtgcc
     7321 tgttccgtca actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg
     7381 acctgggtgg aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc
     7441 tgtttgcgcg gtcgctccat ccgtcggcgt attaatcatc ccgatcgctt gaattatcca
     7501 atgaaacgcg tgggcaaacg cggcgaaggc aaattcgaac ggattagctg ggatgaagcc
     7561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta
     7621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     7681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     7741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     7801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     7861 cgcatgagcg gcggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     7921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ctggtcgtga agatgaatgg
     7981 cttcctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     8041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     8101 accttacccg cagatgcacc aaaaaatggt cactataaag cctatattct tggtgaaggt
     8161 gatgataaca cagcgaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtggac
     8221 cgtatcatca aactggcgcg tgaaattggc acagcaaaac ccgcttatat ctgccagggc
     8281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttgcct
     8341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgaga atcgacctat
     8401 accattacca tagaacgcct gccggtgctt gataatccgg tcaaaacgtc aatctcctgc
     8461 ttcagctgga cggatgctat cgatcatggt ccgcaaatga cggcaatccg cgacggcgtc
     8521 cgcggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacgc gggaaatacc
     8581 ctggttaatc agcgttctga catcaacaaa acgcatgaaa ttctgcaaga cgaatcgaaa
     8641 tgcgaaatga ttgtggtcat cgaaaacttt atgacctcat cggcaaaata tgccgacatt
     8701 ctgctgccag acctgatgac cgttgagcag gaagatatta ttcctaacga ctacgccggt
     8761 aacatgggat atctcatttt cctccagcca gtcaccagcg aaaaattcga acgtaaaccg
     8821 atttactgga tcctgagtga agtcgcgaaa cgtcttggac ctgacgtcta tcaaaagttc
     8881 acagaaggtc gcacgcagga acaatggtta caacatctgt acgccaaaat gcttgccaaa
     8941 gatccagcgt taccgtctta cgacgaactg aaaaaaatgg gtatctataa gcgtaaagat
     9001 cccaatggtc attttgtcgc ctacaaagca tttcgtgacg accccgaggc aaatccactt
     9061 aaaacgcctt ccggtaagat tgaaatttat tccagcaggc tggcggaaat tgcccgtacc
     9121 tgggaactgg aaaaagatga agtgataagc ccattgccag tttatgcctc aacctttgaa
     9181 ggctgggact cccctgaacg tagtaccttc ccactgcaac tgtttggttt ccattacaaa
     9241 tcccgtactc actcgaccta cggcaacatt gatgtcctga aggctgcctg ccgtcaggag
     9301 gtgtggatca accctataga tgcgcagaaa cgtgggattg ccaacggcga tatggtgcgg
     9361 gtgtttaacc atcgtggcga agttcggcta ccagccaaag tgacaccacg cattctccct
     9421 ggggttagcg ctatgggcca gggagcctgg cacgaggcca atatgtctgg cgataaaatc
     9481 gaccatggcg gctgtgtgaa tacgctaacc actctgcgcc cttcaccact ggcgaaggga
     9541 aacccgcagc acactaatct ggtcgagatc gaaaaaatat aacccactac aaccataaat
     9601 tctggcatga cattttgttt gaaaagcaat aagtgagtaa tgatgaaaat ccataccaca
     9661 gaggcgctca tgaaggctga aatcagccgt agaagtctga tgaaaacctc cgcacttggc
     9721 agtcttgcgc tggcaagcag tgctttcact ctgccatttt cccaaatggt ccgggcggca
     9781 gaggctccgg tagaagagaa agcggtctgg agttcctgca ccgttaactg cgggagccgc
     9841 tgtctgttac gtttgcatgt gaaagatgac accgtgtact gggtggagtc tgatacgaca
     9901 ggtgacgacg tctacggtaa tcatcaggtt cgcgcgtgtt tacgcgggcg ctctatccgc
     9961 cgacggatga atcatcctga taggttgaaa tatcccatga agcgcgtcgg caagcgcggt
    10021 gaaggtaaat ttgaacggat aagttgggac gaagccctgg ataccatcag tgataatctt
    10081 cggcggatcc taaaagatta cggcaatgag gctgtacatg tcctgtacgg aacaggcgta
    10141 gatggcggaa acatcaccaa ctcaaacgtc ccgtaccgtc tgatgaactc ttgcggtggt
    10201 tttctcagtc gctatggcag ctacagtacc gcacagatca gtgccgcaat gagttatatg
    10261 ttcggtgcca atgatggcaa cagcccggat gatatcgcca atacgaaact ggtcgttatg
    10321 ttcggaaata acccggcaga aacgcggatg agcggcggtg gtgtcactta ctacgtcgag
    10381 caagcccgcg aacgttcaaa cgcacgcatg atcgtcatcg atccacgtta taacgacaca
    10441 gctgccgggc gtgaagatga atggctgccc attcgcccag gcaccgatgg cgcactggcc
    10501 tgtgcgattg cctgggtact gattactgaa aacatggtcg atcagccatt tctcgacaaa
    10561 tattgtgttg gttacgatga aaaaacgctg cccgccaacg caccacgtaa cgcgcattat
    10621 aaagcctata ttctgggcga agggcctgac ggcatagcta aaacgccgga atgggcagca
    10681 aaaatcacca gcatcccggc agaaaaaatt atccagttgg cacgagagat cggttcagca
    10741 aaacctgctt atatttgtca gggttggggg ccacaacgac attccaacgg cgagcaaacg
    10801 tcccgcgcta ttgccatgct ttccgttctc accggtaacg tcggcataaa cggcggcaac
    10861 tcaggtgtac gcgaaggtag ctgggatctg ggggtagaat ggttcccgat gcttgagaac
    10921 cctgttaaaa cgcagatttc cgtctttaca tggacagatg ccatcgacca tggtaaggaa
    10981 atgaccgcga cccgtgatgg tgttcgtgga aaagaaaaac tggatgtccc catcaagttt
    11041 ttatggtgct acgccagtaa cacattgatc aatcaacatg gcgacatcaa tcacacccat
    11101 gaggtgcttc aggatgacag caagtgcgag atgattgttg gcattgacca cttcatgacg
    11161 gcctcggcta agtattgcga tatcctgttg cccgacctga tgccaacaga gcaagaagac
    11221 cttatctctc atgaatctgc agggaatatg ggctatgtga tcctcgccca acccgcaacc
    11281 tctgcaaaat ttgaacgcaa acccatctac tggatgctga gtgaagtcgc caaacgctta
    11341 ggaccagacg tttatcaaac ctttactgaa ggtcgcagtc agcatgaatg gatcaaatat
    11401 ctccatgcga aaacgaagga acgtaaccct gagatgcccg actacgagga gatgaaaacg
    11461 accgggatct ttaagaaaaa atgcccggaa gaacactacg tcgctttccg cgcattccgt
    11521 gaagatcccc aggcaaaccc gttgaaaaca ccttcgggga aaatcgaaat ttattctgaa
    11581 cgactggcga agattgcgga tacctgggaa ttgaaaaaag atgaaattat tcatcccctt
    11641 ccagcttata cgccaggttt tgatggctgg gacgatcccc tgcggaaaac ctatccactg
    11701 cagttaacgg gcttccacta caaggcgcgt acccactcca gctacggcaa tattgatgtg
    11761 ttacagcagg cctgcccaca agaggtgtgg atcaacccca ttgatgctca ggctcgcggt
    11821 atccgtcatg gcgataccgt gcgggtattt aacaataatg gagaaatgct gattgccgcc
    11881 aaagtgactc cgcgtattct gcctggcgtt accgccatcg ggcaaggtgc gtggcttaaa
    11941 gcggatatgt ttggtgaccg ggtcgatcac ggcggcagta tcaatattct gacctctcac
    12001 cgcccttcac cgctggcaaa gggaaacccg tcgcacagca atcttgtcca gatcgaaaag
    12061 gtttaaagag tagcccatga ccacacaata tggatttttt atcgattcca gccgctgtac
    12121 tggctgtaaa acctgcgaac tggcgtgcaa agattttaaa gatcttggct cggaagtcag
    12181 tttccgccgt atttatgaat atgctggcgg cgactggcag gaagataatg gcgtctggca
    12241 ccagaacgtg ttcgcttatt atctctccat ctcctgtaac cattgcgatg acccagcatg
    12301 tacaaaagtc tgccccagcg gcgcaatgca taagcgtgaa gatggctttg tggtggttga
    12361 tgaagatgtt tgtatcggct gccgctactg ccacatggct tgtccgtacg gcgcaccaca
    12421 gtacaatgct gaaaaagggc acatgacgaa gtgcgatggt tgttattcgc gcgtcgccga
    12481 ggggaaacaa cccatatgcg tcgaatcctg cccgctgcgt gcacttgagt tcggtccaat
    12541 tgaagagctg cgccagaagc acggcactct ggctgccgtc gcgccactgc cgcgcgcgca
    12601 tttcacaaaa cccaatatcg ttatcaaacc taacgccaac agccgcccga ccggcgatac
    12661 cactggttat ctggctaatc cggaggaagt gtaaaatggg aaatggatgg catgaatggc
    12721 cactggttat ctttaccgta ttgggtcaat gcgtagtagg cgcgctgatt gttagcggaa
    12781 ttggctggtt cgccgccaaa aatgacgcag accgtcagcg tattgtccgt ggcatgtttt
    12841 tcctctggct gttgatgggc atcgggttta ttgcctctgt catgcacctg ggatcgccac
    12901 tgcgagcgtt taactcgctg aatcgaatcg gagcttctgg tctgagtaac gagattgctg
    12961 ccggttccat attttttgcc gttggcggac tatggtggct ggtggcggtc atcggtaaaa
    13021 tgccgcaagc gttggggaaa ctctggttac ttgtcagcat ggcgctcggc gtcattttcg
    13081 tctggatgat gacctgcgtg tatcaaatcg acaccgtgcc aacctggcat aacggttaca
    13141 ctacgctggc tttcttcctg acggtattgt tgagcggtcc aatacttgct gccgcaattc
    13201 tgcgggcagc tcgcgttaca tttaatacca caccatttgc catcattagc gttctggcgc
    13261 tgatcgcttg tgccggggtg attgtgctgc aaggtttgtc tctggcgtgt attcactcat
    13321 ccgtacaaca agccagcgca ctggtaccag attacgcctc cttacaggtc tggcgtgtgg
    13381 tattgttatg cgcaggtcta ggatgctggg tttgtccgct cattcgtcgc cgtgaacctc
    13441 acgttgccgg acttattctg gggttaatcc tcattcttgg cggtgagatg atcgggcgtg
    13501 tgctctttta tggcctgcac atgacggtag gaatggctat tgcaggttaa caaattggtg
    13561 cggggcaacc cgcacatttc aggatgttag gaatgaccca tttttcacag caagataatt
    13621 tttctgtcgc ggcgcgcgtg ttgggcgcgc tgttttatta cgctccagag agtgcggaag
    13681 ccgcacctct ggttgcggta ctcaccagtg acggctggga aactcagtgg cctctaccag
    13741 aagcgtcatt agcgccgctg gtgacggctt ttcagacaca gtgtgaagag actcacgccc
    13801 aggcctggca gcgtttgttt gtcggcccgt gggcactgcc gtctccgccg tgggggtcgg
    13861 tctggctgga tcgcgaatct gtgctgtttg gcgattcaac attggcactt cgtcagtgga
    13921 tgcgcgagaa aggcattcag tttgaaatga agcaaaacga accggaagat cattttggtt
    13981 cactgctgtt gatggctgcg tggttggcgg agaatggtcg ccagacagaa tgcgaagaat
    14041 tgctggcatg gcaccttttt ccgtggtcaa cacgttttct tgatgttttt atcgaaaaag
    14101 cagaacaccc tttctaccgt gcactgggtg aactggctcg cttaacgctg gcgcaatggc
    14161 aatcacaact gttgattcct gtcgcggtta aaccgctgtt tcgataggat caccgtaata
    14221 ttgccggatg gtgacgatta cgcgcaatcc ggcaataata ggttacagtg tcacgttttt
    14281 ttatctctta aagcacgcac tgcttttgcg gctggcctct tttgtcgcaa aatagtcgcc
    14341 cgtgtttcat tgcccatttc tgctcatgca tcatctacac atctatccgg atctgcgcac
    14401 tatgttccgc cgtctgctta tcgcaacagt cgtcggtatt ctcgcggcct ttgccgttgc
    14461 cgggtttcgt catgcgatgc tgctactgga gtggttgttc ctcaataatg actccggcag
    14521 tctggtcaat gcagcgacaa acctttcccc ctggcgacgg ttgctaactc cggcgctcgg
    14581 cggactggcg gcgggtttgt tgctgatggg ctggcagaaa tttacccaac aacgccctca
    14641 tgcgccgacc gattacatgg aagcgttgca aaccgatgga cagttcgatt acgcagcaag
    14701 cctggttaaa tcgcttgcct cgctgctggt agtaaccagc ggcagtgcaa ttggtcgcga
    14761 aggtgcgatg attcttttag ctgcccttgc cgcctcctgt tttgcccaac gttttacgcc
    14821 acgccaggag tggaaattat ggatcgcctg tggggccgcg gcgggaatgg ctgcgtccta
    14881 tcgtgccccg cttgctggca gtttatttat agccgaagtg ctgtttggca ctatgatgtt
    14941 ggcctctctc ggcccggtga ttatttccgc cgtcgtggca ttgctggtta gcaatctgat
    15001 taatcatagc gacgcgttac tctacaacgt acaactctca gtgacggttc aggctcgtga
    15061 ctatgcgctg attatcagta caggtgtgct ggcaggtctg tgcggaccac tgttgttaac
    15121 gttaatgaac gcctgtcatc gtggatttgt gagtctcaaa cttgcgccgc cctggcaact
    15181 ggcactaggc gggttgatcg tgggtctgct ttccctgttc acacctgcag tgtggggcaa
    15241 cggctatagc accgtacaat cctttttaac cgccccacca ctgttaatga tcattgccgg
    15301 gatcttcctt tgtaaactgt gtgccgtgct ggcgagtagc ggttccggcg cacccggtgg
    15361 ggtctttaca ccgacgctat ttatcggtct tgccattggc atgttgtatg gtcgtagcct
    15421 gggattatgg ttccctgatg gcgaagaaat tacactttta ctcggattga ccgggatggc
    15481 gacactgttg gcggcgacca cgcacgcgcc gattatgtcg acgttgatga tatgtgaaat
    15541 gaccggggag tatcagctac tccccggttt attgattgcc tgcgtaattg cgtcggtaat
    15601 ttcgcggacg ttacaccgtg actctatcta ccgccagcac actgcgcagc atagctaagc
    15661 ggatgtattg ccccagttca cgctgttcgg cgcgcggcag ataaggcagt tcaccaatga
    15721 gcggtgccgg aagtttttta cccagcacat caatgatttc cgcataatgc gccagtcctg
    15781 ggttgattcg gttagccacc cagccaatga gcggcagccc gtcgttggcg atcgcctgag
    15841 ctgttagcag tgcatggtta atgcaacctt cctgaatacc gacaaccatc aacaccggca
    15901 gttgttcctg cactacccat tcagagagtg gacgcaaatc attcatcaga ctgcgccagc
    15961 cgccagtccc ttctaccacg acatgatcga ctttttcggt caggtttgcc aggccgtttg
    16021 aaatgagggt gtaattgatt gggcaactgt gcgccacgct actttcttct tcgcttaacg
    16081 cgataggatt aactgcttca taaggcagtt cgatggttga aacactctgc aacaccaggg
    16141 catctttatt acgcagccct tcgggtgtct ctttgctccc cttcgctacg ggtttatatc
    16201 ccgcaaccgt ttttccctgg gaggctaacg cttgtagcaa tgcgcgggaa accaccgttt
    16261 tccctacaga agtgtctgta ccggtaataa agaaacgctt cagcatcact aactccaccg
    16321 ttatgcttca caaatataaa ccaggaaaat aattaacctt gaaagtctaa gttatgcttt
    16381 cctggcccaa attgagatag cgcaaatttt ggtagaacag ttaaaaaatg ttaaccctgc
    16441 aacagacgaa tcaacaaaga accgttatac atcgcgtctt ttaccagtgc agcccctgcc
    16501 atcgtgccct ggttagaaaa ctgagtactc tcaacgctga tgtgctgact atacgcagga
    16561 agggcctgct gacggatgct gtctgagatg accgggaaga ggatatctgc cgctttactt
    16621 aacggtgagc caatcagtat tttttgtggg ttaaataaat tcaccatgat ggcaagaatg
    16681 cgccctacat gcgcgcccac cccggtaatg atgtcttttg ccagcagatc gccgcgcaat
    16741 gccgcctgac acaatgagtc cacggttaac ggttgtccat gtaacatcga gctcatggat
    16801 tgattaagac gcagctgtgc cagctcaaga atactgtcca cactggcgat ggtttcgagg
    16861 cagccgtgat tcccgcaata acagcgtttc ccatacgggt cgacctgtgt gtggcctatt
    16921 tccacgagac tactgctgcc tgcgtgtagc agatgaccat cggtaatgac gcccgccccc
    16981 acgttgtgat cgataaccac ctgaatcaca tcgcgcgccc cgcgtgaggc accaaacaag
    17041 gcctctgcca tcgtccatgc gctgatatca tgctgaatat aaaccggtac gccggtatgc
    17101 tgctccagcg cctcgccgag cggcatctct tttacatcct cgtagaacgg catgcgatgt
    17161 acaataccat tttccgtatc aataattccc ggcaaggtta tggcaatcga agttagacgc
    17221 tcaagttttt tctggtggcg aataaaaaac tgatcgatat gggaaatgat acgatccagc
    17281 aatggcgagt catcttttaa cgccagttcc tgcgactctt ccaccaccag tttgctgctc
    17341 agatcgcgca gagcaaggaa aatctccccg cgactaatgc gcagagaaag atagtgccag
    17401 gcttcagttt caaccaccag ccccaccgcc ggacggccac ggttccccgc ttctttgatt
    17461 tccagctctt gcaccaggtg tgcttcgagc atctcacgga caattttagt gatactggca
    17521 ggagccagct gcgccagacg ggaaagatcg atacgcgaga ctggaccaag ctgatcaatc
    17581 aggcgataaa ccgcgcccgc gttggtctgc tttatttgat caatgtgccc aggctggttt
    17641 tcagcaacca ccgcatactc cctatatttt cgcgctccga aataatctgt aggctatggt
    17701 gaagcacttc aatacgtgtc gtcaaatttt tacttaggca tgtgattaac agcacatttt
    17761 tcgggctttt tcgctgaaat ttccctcacc tgagagcatt cagcagatga atacgaaagt
    17821 ttcgcgcagc tggactttgt tcatgatgtt tcggccagac caaccacatt tcagaaaccg
    17881 catcctcttc tgcaatcggc acccagcgca tttcgttgag ctgaacacgt ttaaatgacg
    17941 caggcaaaat tgaaacaccc agaccggcgg aaaccagacc gatgatggtc attgcctcgc
    18001 ccacctcctg agtgatgacg ggcgtcaaat ggtaacgtcg catcagcccg agaatatctt
    18061 catacagccc tgtcccgacg tgcggatcaa aaaagacgaa gggttctttc gccagttcag
    18121 ccagcgttac attcgggtta tttgccaggg gatgatcgtg cgggatcatc gccataagcg
    18181 gttcatggac gatgactgcg tgttcaagcg tctccggtaa cgctgtgtta cgcagcaatc
    18241 ccatatccag cgttccttca atgagcggag cgatttgctc acgagtgttc atttcgcggg
    18301 tttgtaaatg gacatcggga taatcacggc gaaacagcga taacgtatcg gacaccgccc
    18361 gaataaaagg agcggacgaa gtaaaaccaa tgcgcaactc ccccgcttcc ccctgatgca
    18421 gtctttcagc gcgagcggca gcgtcatcta ccatagacag gatttgccga ctatctgcaa
    18481 gaaactgttt tcctgctgcc gtcagcaata cactgcgatt agttcgtgcc agcagtcggg
    18541 caccaatttg ttgctccagt gcctgaatct gctgacttag cggtggttgc gaaatattca
    18601 ggcgggcagc ggcgcgcccg aaatgcagct cttccgcaat agcaacaaag taacgcagat
    18661 gacgaagttc aatattcata tttaaaacat cttatttgag attattaata tattagacag
    18721 aacaattcga ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat
    18781 ttcaagtgag ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa
    18841 gcatttctca gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc
    18901 tggcgctgtt ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc
    18961 ttccggtgct ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca
    19021 tttccacggc gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg
    19081 gtcgcaaacc agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga
    19141 caatgatgac cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa
    19201 gtggcgtggc agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg
    19261 ccttttcgat ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa
    19321 ttagtggtgt cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt
    19381 tcgcgctggc ctcggcgttg atgttctgga aaatccttcc ggaatcacgc cattttcgcc
    19441 cgacttcgct gcgccctaag acgttattta tcaactttcg tctgcactgg cgtgaccggg
    19501 gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta
    19561 attacatcgg ctatcggttg atgctttccc cctggcatgt cagtcaggcc gtggttggct
    19621 tattatcgct ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca
    19681 cccgctacgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac
    19741 tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag
    19801 gattcttcgc tgcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta
    19861 aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga
    19921 cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc
    19981 tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa
    20041 aaataagccc ggactgcggt aactacccgt ccggacttat tgcttgctca aaccaacgtt
    20101 aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc
    20161 aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc
    20221 gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat
    20281 tggtagctaa atttccctta agtcacaata cgttattatc aacgctgtaa tttattcagc
    20341 gtttgtacat atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg
    20401 atatagttat ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga
    20461 caatgaaaaa agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct
    20521 ttgctgcaga gactgcgaac acacctgctc cgactgcgac gaccaccaaa gcagcgccgg
    20581 cgaaaactac acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg
    20641 ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg
    20701 cagcgaagaa acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac
    20761 ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt
    20821 gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg
    20881 ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct
    20941 ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt
    21001 atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat
    21061 tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga
    21121 cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt
    21181 gccagtgaat gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag
    21241 cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg
    21301 tttattgacg ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa
    21361 taaaggtctt tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg
    21421 aaagcggtta aaagcagatg gggatggttg gattgttcct cccgcagctg caccatggga
    21481 cttcggcttg attgtgctgc gtaatccccc ttctggcatt acaccgttgc cgttatttga
    21541 gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc
    21601 gggatatcct gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg
    21661 ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc
    21721 gcctctgatg ttgcataccg atgactgctg gcaattaatt ggggtgcaaa gttcggctcc
    21781 tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga
    21841 caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg
    21901 tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca
    21961 gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat
    22021 accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttta cggcctgaga
    22081 aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg
    22141 aaagccgtca gaaaa
//
LOCUS       DAHBHC010000082        21694 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHBHC010000082 DAHBHC010000000
VERSION     DAHBHC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.082"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(117..1187)
                     /gene="dadX"
                     /locus_tag="M2A35_003843"
     CDS             complement(117..1187)
                     /gene="dadX"
                     /locus_tag="M2A35_003843"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCB7200087.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(1197..2495)
                     /gene="dadA"
                     /locus_tag="M2A35_003844"
     CDS             complement(1197..2495)
                     /gene="dadA"
                     /locus_tag="M2A35_003844"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCB7200088.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            2825..4357
                     /locus_tag="M2A35_003845"
     CDS             2825..4357
                     /locus_tag="M2A35_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCB7200089.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(4409..5128)
                     /gene="fadR"
                     /locus_tag="M2A35_003846"
     CDS             complement(4409..5128)
                     /gene="fadR"
                     /locus_tag="M2A35_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCB7200090.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            5350..6891
                     /gene="nhaB"
                     /locus_tag="M2A35_003847"
     CDS             5350..6891
                     /gene="nhaB"
                     /locus_tag="M2A35_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCB7200091.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7037..7567
                     /gene="dsbB"
                     /locus_tag="M2A35_003848"
     CDS             7037..7567
                     /gene="dsbB"
                     /locus_tag="M2A35_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCB7200092.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(7613..8881)
                     /gene="umuC"
                     /locus_tag="M2A35_003849"
     CDS             complement(7613..8881)
                     /gene="umuC"
                     /locus_tag="M2A35_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCB7200093.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(8881..9300)
                     /locus_tag="M2A35_003850"
     CDS             complement(8881..9300)
                     /locus_tag="M2A35_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCB7200094.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            9673..10583
                     /gene="hlyE"
                     /locus_tag="M2A35_003851"
                     /pseudo
     CDS             9673..10583
                     /gene="hlyE"
                     /locus_tag="M2A35_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(10790..11236)
                     /locus_tag="M2A35_003852"
     CDS             complement(10790..11236)
                     /locus_tag="M2A35_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCB7200095.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(11328..11987)
                     /locus_tag="M2A35_003853"
     CDS             complement(11328..11987)
                     /locus_tag="M2A35_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCB7200096.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(12059..12352)
                     /locus_tag="M2A35_003854"
     CDS             complement(12059..12352)
                     /locus_tag="M2A35_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCB7200097.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            12594..12995
                     /locus_tag="M2A35_003855"
     CDS             12594..12995
                     /locus_tag="M2A35_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCB7200098.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(13115..13483)
                     /locus_tag="M2A35_003856"
     CDS             complement(13115..13483)
                     /locus_tag="M2A35_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200099.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            14003..14698
                     /gene="minC"
                     /locus_tag="M2A35_003857"
     CDS             14003..14698
                     /gene="minC"
                     /locus_tag="M2A35_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCB7200100.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPTPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            14722..15534
                     /gene="minD"
                     /locus_tag="M2A35_003858"
     CDS             14722..15534
                     /gene="minD"
                     /locus_tag="M2A35_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCB7200101.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            15538..15804
                     /gene="minE"
                     /locus_tag="M2A35_003859"
     CDS             15538..15804
                     /gene="minE"
                     /locus_tag="M2A35_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCB7200102.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(16176..16520)
                     /locus_tag="M2A35_003860"
                     /pseudo
     CDS             complement(16176..16520)
                     /locus_tag="M2A35_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(16555..16674)
                     /locus_tag="M2A35_003861"
     CDS             complement(16555..16674)
                     /locus_tag="M2A35_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCB7200103.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(16635..16820)
                     /locus_tag="M2A35_003862"
     CDS             complement(16635..16820)
                     /locus_tag="M2A35_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200104.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            16921..17094
                     /locus_tag="M2A35_003863"
     CDS             16921..17094
                     /locus_tag="M2A35_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200105.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            17096..17440
                     /locus_tag="M2A35_003864"
     CDS             17096..17440
                     /locus_tag="M2A35_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCB7200106.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            17444..17779
                     /locus_tag="M2A35_003865"
     CDS             17444..17779
                     /locus_tag="M2A35_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCB7200107.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSTKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(17836..20157)
                     /locus_tag="M2A35_003866"
     CDS             complement(17836..20157)
                     /locus_tag="M2A35_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7200108.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(20274..20483)
                     /locus_tag="M2A35_003867"
     CDS             complement(20274..20483)
                     /locus_tag="M2A35_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200109.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(20883..21101)
                     /locus_tag="M2A35_003868"
     CDS             complement(20883..21101)
                     /locus_tag="M2A35_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200110.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(21233..>21694)
                     /locus_tag="M2A35_003869"
     CDS             complement(21233..>21694)
                     /locus_tag="M2A35_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCB7200111.1"
                     /translation="CLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFV
                     IALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISI
                     VAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         5719 a         5396 c         4967 g         5612 t
ORIGIN      
        1 atggacacta tcatgggcaa tggctctgtc acattaacaa ctacagttgc tgaccagccg
       61 gatggatgat tttttcgcaa ccagaagacg ctgtctccga tccggcttac aacaagttac
      121 actgtcacaa ccgggacgcg taacgccagc gcgcacatca actcatagcc caccgttccg
      181 gcagcggcgg cgacatcatc aattttgatc tccttgcccc acagctcaac cggcgtacca
      241 atccccgcct gcgggcaagg cgttaaatcg accgccagca tatccatcga gacggtcccc
      301 accgtcatgg tgcgcacgcc gtccactaaa acaggggtac cggtaggcgc gtggcgcgga
      361 taaccgtcgg cgtaccctgc ggcgacaatg ccgattcgct gttcatcgcg cgcagtatag
      421 cgaccgccgt agcccacacg ctcacccgct tttagcgtct ggacaccaat aatctcactg
      481 cttagcgtca tcaccggacg taatccggta ttggcgatat cacgccactg accggacggc
      541 gaagcgccat acaaaataat gccaggccga acccagtcaa aatgcgcttc cgggtgccac
      601 agagtcgccg ccgaattgga caacgaacgc cgacactcca gcccttccgc cgcctgctca
      661 atacgcgcca tcgcgctgga aattccatca ggatgttccg cttcggcaaa atgcgacatc
      721 agggtcatct cgccaacatt cgccattgcc cgcaactgct gccagacggt aagcacgcga
      781 tcgggctgga agcccaaccg attcatccca ctgttcactt taagataaat atccaacggt
      841 gcttttagcc gcgcattttg cagtgctttg agctgccagt tgctgtgtac gcaggtggtc
      901 aggcggtgct ggtcataaat ctccagatcc tgagcatgga aaaatccttc cagcatcagg
      961 atcggcccct tccagccgcg ctcacgtaac gttattgcct cttccaggtt aagtaatgca
     1021 aagccatcgg tggccccgag cgcgctccag atacgctcaa taccatgccc gtaagcgttc
     1081 gcttttacca ccgaccagac gcgcgcgtgc ggcgcggcct ggcggacaat ggacagattc
     1141 tgttttaatg cctgcagatc gaggctggcc tgtatcggac gggtcatctc gtttccttag
     1201 ctgtgtgcgc catgtaaatg gcccggacgt gatggcgtaa atccacggct gtagcgcgct
     1261 acgcttagat cctcatatgg gatcgctggc gtgcgaccag agagcagatc gcttaacaac
     1321 tgaccggaac cgcaagccat cgtccagccg agcgtgccgt gaccggtatt cagccacaga
     1381 tttttaaagc gtgtacgccc gacaaccggc gtaccgtctg gcgtcatcgg gcgcaaaccc
     1441 gtccagaaag tcgcctgctc gacatgaccg ccgcgtggat agagatcgcg aaccaccatt
     1501 tccagcgttt cacgacgcgg ttgcaacagc tcggtgttaa aaccaacaat ttccgccatt
     1561 ccgccaacac gaatgcggtt atcgaaacgg gtaatggcga ttttgtaggt ttcatcaaga
     1621 atagtggata ctggcgcacc atcttcctgc gcaattggaa tagtcagcga gtagcctttc
     1681 agcggataca ccggaatatc aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac
     1741 gccatcacat acgcatcggc cttaatcact tcatcgccac acttcacgcc gtagatttgc
     1801 tcgccgtcgc aaagcagttg gtcaacgggc gtattaaagc ggaatttaac ccccgcctgc
     1861 tctgccatcc gcgccagatt ctgggtaaac agctggcagt ctccggtttc atcattgggc
     1921 agttgcagac cacccgtcag tttgtgcgct acttctgcca gcgcgggctc cacttccgcc
     1981 aggcggctgg attccagcag ctgatacggt acgccggcat cttccagcac ggcgatatcg
     2041 cgggtcgcat tttcatactg ttgttcggta cggaacagtt gcagcgtccc accctgacgc
     2101 ccttcatact gaatattggt ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc
     2161 gccagacgca ccatccgccc tttgttttcc atgtagtggc tggtgtcgca gttacgtaac
     2221 atttgccaca tccatttcaa ctgaaactgc gtaccgtcga gacggaccgc cagcggcgca
     2281 tggcgctgga acatccattt gatcgctttt aaaggcacac ctggtgccgc ccacggtgca
     2341 gcatatccgg gggagatttg cccggcattc gcggcgctgg tttccagggc ggcccccggc
     2401 tcccgatcaa tgacggtgac ctcatgtcct gcctgattta agtaccaggc gctggcaacg
     2461 cctaccacac cacttcccag tatgacaact cgcatagcca ctccgataac ggtagataca
     2521 gtaaaagaat aacaatctaa ttacatcttg ataactcagt tgaaaatatt attcaacata
     2581 cggctttttt atggtgagct ggctcacatc tccctgactc tgcggcaata tggcgtcttt
     2641 agtatctcct gctgaacgcc ctttttatcc agcataaaat tccgttcaga agcggattag
     2701 tggcactctg ataataagaa atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac
     2761 aggtgttcta tgcttgaaat gaggtgctcc acacagagag cgccgacaac aatgaggtgc
     2821 gcgtatggcg acgatcgatt ctatgaataa ggacaccaca cgtttgagcg atggacccga
     2881 ctggacgttc gacctgctgg atgtttatct ggcagagata gaccgggtgg cgaaactcta
     2941 ccggctggat acctacccgc accagattga agtgataacc tcagaacaga tgatggatgc
     3001 ctactccagc gtcggcatgc caattaacta tccgcactgg tcattcggta aaaagtttat
     3061 cgagactgaa cggctgtata agcacggtca gcaaggactg gcctatgaaa tcgtcattaa
     3121 ctctaacccg tgtatcgctt acctgatgga agagaacacc attaccatgc aggcgctggt
     3181 gatggcacac gcctgctatg ggcataactc tttctttaaa aacaattact tattccgcag
     3241 ttggaccgac gccagttcga ttgtcgatta tctgattttt gcccgtaaat atattaccga
     3301 gtgcgaagaa cgttatggcg ttgatgaagt agaacggctt ctggactcgt gccacgcgct
     3361 gatgaactac ggcgtggacc ggtacaaacg cccgcaaaaa atctcgctgc aagaagagaa
     3421 agcccggcag aaaagtcgcg aagagtatct acaaagtcag gtcaatatgc tctggcgtac
     3481 cctgccgaag cgcgaggaag agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc
     3541 acaagaaaac ctgctctatt ttatggagaa aaatgcgcca ctgctggaat catggcagcg
     3601 tgaaatcctg cgtattgtgc gtaaggtgag ccagtatttt tatccgcaaa aacagactca
     3661 ggtgatgaac gaaggctggg cgaccttctg gcactatacc atccttaacc atctgtatga
     3721 tgaagggaaa gtaacggaac gttttatgct ggagtttttg cacagccaca ccaatgtggt
     3781 cttccagccc ccctataaca gcccgtggta cagcggcatc aacccatatg ccctcgggtt
     3841 cgccatgttc caggatatta aacggatttg tcagtcgcca acggaagaag acaaatactg
     3901 gttcccggat atcgccggtt ccgactggct ggaaacgctg catttcgcga tgcgtgattt
     3961 caaagatgag agttttatca gccagtttct gtcaccgaaa gtgatgcgtg atttccgctt
     4021 cttcaccgtg ctggatgatg atcggcataa ttatctggag atttccgcta ttcataatga
     4081 agaaggttat cgggagatcc gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga
     4141 gccgaatatt cagatctgga acgtggattt gcgcggcgac cgttcgctga cgctgcgtta
     4201 cattccacat aatcgcgcac cgctggatcg ggggcgcaaa gaagtgctga agcatgtgca
     4261 tcgcctgtgg ggatttgatg tgatgctcga acagcaaaac gaagacggca gcatcgagtt
     4321 gctggaacgt tgcccgccaa gaatgggaaa tctgtaaaaa caacaaaaaa cccctcgttt
     4381 gaggggtttg ctctttaaac ggaagggatt atcgcccctg aatggctaaa tcacccggca
     4441 gatttttctg catccggtgc caaatctcgc cactctcatg cccatagcga cgcactgttt
     4501 cgtacacctg atcgtgcgcg ccttcactgc acaacgccga cagtttgtgg tagaagccca
     4561 gcgccagact gcgcgcttcc ggattggcga aatagtgacg accaatgcgc gtatacagcc
     4621 ctttcatccc gttaaggatc agaccgtaaa tcgggttgcc ggaagcaaac gccaggccgc
     4681 ggaatatgtt gtaatccagc tcggcaaagg catcggcgtg atcggccact tcattagcgg
     4741 tagccagcac ttcctgcgct ttatcgggat gctgacgaaa cgcggtgcga ataaaaatag
     4801 tggaaatatt ggtacgcacc gacagcaaat tatcaataag ctgcggcaca ctttcgtgat
     4861 ccagtcgcgc cagtgtttca aggatattta aaccagaggt ttcccagaaa ttattcacct
     4921 tcgtcggctt gccatgttga atggtcaacc agccatctcg tgccagacgc tgtaacactt
     4981 cacgtaacgt agtacgcgtt acgccaatta attctgaaag ttcacgttct gcgggcaaaa
     5041 tagtcccggg agggaagcgg ttattccaga tactttcaat aatgtactct tccgcgaaac
     5101 ccgccgggct ttgcgcctta atgaccatag tgagatttcc ataacacagc aaaacaaagt
     5161 tggactcatc ataccagagg gctaactacg ctgatagcag acaaaaagaa gaaaaaaggg
     5221 atcaaggctt cattttccgc atctctttcc ccttgactat caagacttgc ctcactcctg
     5281 cctgaccgtt aagctttgcg tttaaacata aaaacatcat tgattatttg taaggtaagg
     5341 gaaaccatca tggagatctc ctggggccgc gcgctatggc gcaatttttt gggccagtcc
     5401 cccgactggt acaaactcgc cctcatcatt ttcttaatcg taaacccgtt aattttcctc
     5461 atcagccctt tcgtcgcggg ctggttgctg gtagcggaat ttattttcac tctggcgatg
     5521 gccctgaaat gctacccgct gctccccggt ggtctgttgg ctatcgaagc ggtattcatc
     5581 ggcatgacca gcgcggaaca cgtccgtgaa gaggtggcgg caaatcttga agtcttgctg
     5641 ttattgatgt ttatggtggc gggtatctat tttatgaaac agctgttgct gttcatattt
     5701 acccgtttgc tgttaagcat tcgctccaaa atgctgctgt cgctctcttt ttgcgtggcg
     5761 gcagcgttcc tctccgcgtt cctcgatgcc ttaaccgtcg tggcggtggt gatcagcgtt
     5821 gctgtcggtt tttatggtat ttatcaccgc gtagcctctt cccgtaccga agataccgac
     5881 ctgcaagacg atagtcatat cgacaagcat tacaaagtgg ttctggaaca gttccgtggc
     5941 tttctgcgta gcctgatgat gcatgccggt gtcggcactg cattaggcgg cgtgatgacc
     6001 atggtgggcg aaccacagaa cctgatcatc gctaaagcgg ctggctggca ttttggcgat
     6061 ttcttcctgc gcatgtcgcc ggtgaccgtt ccggttctga tttgtggcct gttaacctgc
     6121 ctgctggtag agaagctgcg ttggtttggc tacggtgaaa cgctgccgga gaaagtccgc
     6181 gaagtgttgc aacagtttga cgatcaaagc cgccaccagc gtacccgtca ggataaaatc
     6241 cgtctgattg tccaggcgat tattggcgtc tggctggtga ctgcgctggc gttgcatctg
     6301 gcggaagttg gcttgattgg tttgtcagtc attattctgg cgacatcatt gaccggtgtc
     6361 accgatgaac atgctatcgg taaagccttc accgaatctc tgccattcac cgcactgttg
     6421 acggtgtttt tctcggtagt cgcggtgatt atagaccaac aactgttttc gccaattatt
     6481 cagtttgtgt tgcaggcatc ggaacatgct cagctgtcgc tgttctatat tttcaacggt
     6541 ctgctgtcat ccatttcgga taacgtcttc gtggggacga tttatatcaa cgaagcgaaa
     6601 gcggcaatgg aaagtggcgc tatcacgttg aagcaatacg agctgctggc ggtcgccatt
     6661 aataccggta ccaatctgcc ctccgtcgct acgccgaacg gtcaggctgc gttcctgttc
     6721 ctgctgacct ctgcactcgc gccattgatt cgcctctctt atggccgcat ggtgtggatg
     6781 gccctgcctt acaccctcgt actgacactc gtcggcttgc tctgcgtcga gtttacgctt
     6841 gcccctgtaa ccgaatggtt tatgcaaatg ggctggatag caacgctttg ataacaactt
     6901 accgggcata taaatgcccg gtttgccttt tcgcccgata attgtccaat tgcttcctat
     6961 ttttgctgcc tcctggtggc gcagcgaatg aattggttta aactgcgcac tctatgcata
     7021 ttgcagggaa atgattatgt tgcgattttt gaaccaatgt tcacaaggcc ggggcgcgtg
     7081 gctgttgatg gcgtttactg ctctggcact ggaactgacg gcgctgtggt tccagcatgt
     7141 gatgttactg aaaccttgcg tgctctgtat ttatgaacgt tgtgcgttat tcggcgttct
     7201 gggtgctgcg ctgattggcg cgatcgcccc gaaaactccg ctgcgttatg tagcgatggt
     7261 tatctggttg tatagtgcgt tccgcggtgt gcagttaact tacgagcaca ccatgcttca
     7321 gctctatcct tcgccgtttg ccacctgtga ttttatggtt cgtttcccgg aatggctgcc
     7381 gctggataaa tgggtgccgc aagtgtttgt cgcctctggc gattgtgccg agcgtcagtg
     7441 ggatttttta ggtctggaaa tgccgcagtg gctgctcggt atttttatcg cttacctgat
     7501 tgtcgcggtg ctggtggtga tttcccagcc gtttaaagcg aaaaaacgtg acctgttcgg
     7561 tcgctaatcc cttcagcgct cctgcgggag cgcttttttc ctgccgctat atttatttga
     7621 cccgcagtaa atcagaactt cgcgttgtat aacgtggtga aagcatggca cgcttcatct
     7681 gccattgttg ctggatcccc tgcccggcaa aatagagtgt tcctctgccc tctttagcat
     7741 tcagtgtatc cattaccgtc atcaattgct cactcccggg gcgcggtgcg ttgtcatcga
     7801 ataaattgag ctgcgcgact ccctgactga agaaatcccc cagcatcacg cccgcttttt
     7861 ggtagcgatg gcccgcttgc cagatggcat ccagagatcg cgtagcagcg ttaatgatat
     7921 ccctgctgtc ctgagtgggc gtcagcagtt ttaccgacgc gctattgccg taataaggtt
     7981 cattgagcgc aaatggtgac gtcttaataa atgtggagat aaaccgacaa tattgatgct
     8041 cgctgcgaag tttttccgcc gcccgggcag cgtaactaca aatggcctgc cgcatcgatg
     8101 gataatccgt gatgcgttca ccaaacgagc gggaacagat aatttcctgc ttcgtcggtg
     8161 caaactcttc cagttgcaaa cagggttcgc cgcgcagttc acgcaccgtt ctttcgagca
     8221 cgacattaaa atgtttacgg ataaaccgga tatctgtatc cgccaaatcg agaacggttt
     8281 tgatccccat cgcgtccagt tttttgctga tccgccgtcc aatcccccag acgtcatcca
     8341 cggggagagc agacattaat ttacgctggc gttccagatt tgataaatcc accaccccac
     8401 ccgtctgccg ctgccatttt tttgccgcat gattggcaag cttagccaac gttttggtct
     8461 gggcgatccc cacaccaaca gtaagatggg tacgttgtag caccgttgcg cgaatttctc
     8521 tgccaaaatc agtcagatcg cgacaattac gcacacctgt cagatcgcag aatgcctcat
     8581 caatactgta aatctcgacg cggggcgata gttcttccag cgtcgacatc acccgattgc
     8641 tcatgtctgc gtaaagctca taattgctgc taaagcaaac cacgccacag cgacgaaaca
     8701 gatctttttg tttgaaccag ggatcgccca ttttaacacc cagcgccttt gcctcagcgt
     8761 ttcgggcgat aacgcaaccg tcattattcg atagcacaac caccggttta ccccataaat
     8821 cagggcgaaa caccgtctca cagctggcat aaaacgcgtt tacatcacag agagcaaaca
     8881 tcagcgcatc gccttaacga cgtggatcac cacaccaaag acatccagcg tatcttcgct
     8941 actgatggta atgggcgagt acgcactgtt catgggaata agctgtaccg tcgggcgtag
     9001 ttgcaatttt ttcaccgtaa actcgccgtc aacagcagcg atgacaatat caccatggct
     9061 ggcggtaata gcgctatcga caatcagtaa atcaccgtca ctaattccac catcaatcat
     9121 agaatcacca cttgctttga cgaagtaagt cgcgctggga tgctggatca acagttgatt
     9181 cagatcgatg cgctgttcaa cgtaatctgc tgccggtgaa ggaaagccac actgaacaag
     9241 atcgctaaat agcggaaaag tcacaatttc gcggagatcc gcaggcttga taaacaacat
     9301 aataatctgc ctgaagttat actgttttta tatacagtag cctgttcttg ccagcagatc
     9361 aatactgatt caggctatca atatttgtcg ctgcatagac tgctgatttt tcgttctctt
     9421 atcctgtgct cacgtggcct tctggcgacg acgctcatcc agcagaaatg aaaaatatca
     9481 cccggcaaaa aaatagaata gaagcattcg ccataatgac attaaacatt gtttgatatt
     9541 tatcatatta atagaaataa agacattgac gcatcccgcc cggctaacta tgaattagat
     9601 gaagtaaaat ttattaatag ttgtaaaaca ggagtttcat tacaatttat atatttaaag
     9661 aggcgaatga ttatgactga aatcgttgca gataaaacgg tagaagtagt taaaaacgca
     9721 atcgaaactg cagatggagc attagatctt tataataaat atctcgatca ggtcatcccc
     9781 tggcagacct ttgatgaaac cataaaagag ttaagtcgct ttaaacagga gtattcacag
     9841 gcagcctccg ttttagtcgg cgatattaaa accttactta tggatagcca ggataagtat
     9901 tttgaagcaa cccaaacagt gtatgaatgg tgtggtgttg cgacgcaatt gctcgcagcg
     9961 tatatttttc tatttgatga gtacaatgag aagaaagcat ccgcccagaa agacattctc
    10021 attaaggtac tggatgacgg catcacgaag ctgaatgaag cgcaaaaatc cctgctggta
    10081 agctcacaaa gtttcaacaa cgcttccggg aaactgctgg cgttagatag ccagttaacc
    10141 aatgattttt cagaaaaaag cagcttttcc agtcacaggt agataaaatc aggaaggaag
    10201 cgtatgccgg tgccgcagcc ggtgtcgtcg ccggtccatt tggattaatc atttcctatt
    10261 ctattgctgc gggcgtagtt gaagggaaac tgattccaga attgaagaac aagttaaaat
    10321 ctgtgcagaa tttctttacc accctgtcta acacggttaa acaagcgaat aaagatatcg
    10381 atgccgccaa attgaaatta accaccgaaa tagccgccat cggggagata aaaacggaaa
    10441 ctgaaacaac cagattctat gttgattatg atgatttaat gctttctttg ctaaaagaag
    10501 cggcaaaaaa aatgattaac acctgtaatg agtatcagaa aagacacggt aaaaagacac
    10561 tctttgaggt acctgaagtc tgataagcga ttattctctc catgtactca aggtataagg
    10621 tttatcacat ttaaaaacac agacgttttt taaagattga gagttacctg ttgataataa
    10681 ggtatcgggt aactctcatt atgacagagc gctttcaaaa acagggctac gaaatggcga
    10741 gcatccagac gtatattaag agagccccca tcaacatcta ttaaatttat cactgcgccc
    10801 agtcaggttt atttaagata tgatcctgcc agtcaatcac ttctgattct ttcactgcga
    10861 tatgacgcac agagatacgt tcaccatgca ttgccgcttt cgaaccagta agtagcggat
    10921 gccacgcagg taaatcttta ccttccgcca gcaaacgata agcgcaggtc attggcagcc
    10981 attcgaatgt tggcagattt tcacgggtta atttaatgca gtcgggttca aactcgaaac
    11041 gacgttcgta gttccgacat tgacaagttt taatattgag ctggcgacag gcgacgttag
    11101 tgaagtagat ttcgtcggtg tcttcatcca tcagtttatg caggcaacac tgaccgcaac
    11161 catcacacaa cgactcccat tccgcatcgc tcatttcgtc cagggtttta ctttgccaga
    11221 aaggtacatc gctcatcagg tgttccgcca ttacgttaaa acgcacctta taaccagtct
    11281 ggcacagcga tgcaagtttt gccgccgctt tcaggcggca aaaagtatta caaaacgcga
    11341 gttgtcaaag aatgcccatc gaaagtgact gtcagctcat caccgctttg caacgggccg
    11401 acgccatcag gcgtgcctgt caacacaacg tcaccggcct tgagggtaaa aaacttgctc
    11461 atataagcga tcaacggaac gattttatgg atcatgtccg cagtcgtacc ttgctggcgt
    11521 tgttcgccgt ttacgctcag gcccagcgtt gtattttgtg gatcgccggt aaattccgcc
    11581 gcggggataa acccggaaag cggacaagag ttatcaaacg ctttagcctt ttcccacggc
    11641 tgcccggctt tcttcatttt tccctgaaca tcacgcaatg tcagatcaag cgccacgccg
    11701 taaccggcaa tggctttgcg gacatgctct tccgttgcct gacgcagcgt cgcgccaatc
    11761 aacaccgcca gttcgacttc atgatgaact gaaccgaaat cggatgggat cgccaatggc
    11821 tgccgcagat cgcacagtgc cgtttctggt ttaataaaca gcactggctc ttcgggcact
    11881 gcgctgccca tctctttaat atgtttggca tagttactgc caacacagac tactttactc
    11941 accggataat ccagcagcgc accttgccag ttgtgatgtt gatacataac gttcccctca
    12001 ccttatcgtg gatatgaaaa cccgcgccga caaagataat gccaccgccg gatatcggtt
    12061 atttgttagt gtcgtctgtt ttctgcccca tgacggaaag atgttgcttc agcaaatctt
    12121 cgggtggtgg cggtaactgc aaatagtaac cttgctcggt taatgccagt ttaacctttt
    12181 caatatcagc attgaccagt ttcttgcgcc catccagcgg cagaatcatc gctaactgag
    12241 gctgaccaaa acctttcatc agttcctcag gaacacgcga aaaatcgtct tttttttcga
    12301 catataaata ggtctggtca cgcttgctgc ttcgataaat cacacaaaac atacttttac
    12361 tctttaagat gccaggtttt ggcataagct aacatcaatg aaacccgcgc cacaagccag
    12421 ccagctgtag atttctgcgg tttgttgagt ccatgcccct ttataagtaa tccccccggc
    12481 aaaaaatgcc gcgccgggat tttaccgaac gcagaaaagc cactggaatt ctcccgtaag
    12541 ggattgttat attataacag ttcatcgtac tcattctgaa caggagacta ccaatgaaaa
    12601 tcaagagcat cagtaaggct gtattgctgc ttgcactgct aacctccacg agctttgctg
    12661 cgggtaagaa tgtcaatgtt gagttcagca aaggccatag cagcgctcag tactccggcg
    12721 aaataaaggg atacgattac gatacatata ccttctatgc caaaaaaggt cagaaagtgc
    12781 atgtgagtat ctctaatgaa ggcgctgata cttacctctt cgggccagga atcgatgatt
    12841 cagttgattt atccagatat tcaccagaac tcgatagtca cggacaatac tcactccctg
    12901 cttctggcaa atatgagctg cgagtgctgc aaacccgaaa cgacgcccgt aaaaacaaaa
    12961 ctaaaaaata taacgtcgat attcaaatta agtaaatgtc aaccgacggg gactctcccc
    13021 gtcataaacg ttaacgcccc actcctctct gtcgctcaag aggttaatga cgaatagcag
    13081 acaatgccga atagaacata atgtaaacaa ggcatcactc acgacacata aataacatct
    13141 ttgtggtgat ttgattgatt tttccactcc gcttaccatc cacaccaaaa taacgatcat
    13201 cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg
    13261 gatcgtagcc ttgtaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg
    13321 ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc
    13381 acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc
    13441 ccacacctga caatccgata taaaaaatac gcatcatatt catagcacat cctgataatc
    13501 caaccgtaca acacttatgc tcatttcatc atcaatccgt aatcaaaacc gctgacctgg
    13561 acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta tgatagagca
    13621 attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgccg caaaaaatgg
    13681 aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg gatatttaaa
    13741 caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa taagaacaat
    13801 caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat gaatcagcgc
    13861 catttatcac agaatagact tttactctga ataaatggga gggtgacttg cctcaatata
    13921 atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa
    13981 tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag gcagtagctt
    14041 cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc aggcgctgga
    14101 agacaaaatc gctcaggccc ccgcattttt aaaacatgcc cccgttgtac tcaacgtcag
    14161 tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg caaccggttt
    14221 gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaagat
    14281 ggggctgcct atcctgacgg aaggaaagga aaaagcgccg cgtccaactc ccgcaccaca
    14341 ggctccagcg caaaatacaa cgccggtcac aaaaacgcgt ttaatagata ccccggtgcg
    14401 ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc acgttagcgc
    14461 tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc
    14521 gcttgcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc tgatggcgga
    14581 actggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag aattttatgg
    14641 caaagcggcg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc
    14701 cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg caaagggggt
    14761 gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa gggaaagaaa
    14821 actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat gggttgtgaa
    14881 cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg
    14941 ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca aacacgcgat
    15001 aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa agcgatggat
    15061 tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt aatggcactc
    15121 tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt acgcgactct
    15181 gaccgtattt taggcattct ggcgtcgaaa tcacgccgcg cagaaaatgg cgaagagcct
    15241 attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag aggtgacatg
    15301 ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag
    15361 gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga cattaacgcc
    15421 gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga acgtcctttc
    15481 cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg ataagttatg
    15541 gcattactcg atttctttct ctcgcggaag aaaaacacag ccaacattgc aaaagaacgg
    15601 ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta tctgccgcag
    15661 ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc tgagatggta
    15721 accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa cgtgacctta
    15781 ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt caaggcagag
    15841 tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc ggcgagtatt
    15901 cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag gctgttccca
    15961 cgcacagaac aaaatgttta tgcctggttg agtaaataac cctgttgtta gtgtggatat
    16021 actgggagat gataaccccg caatttcatt attcattgtt ctcccatagg atgaggtttc
    16081 ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat cctgtaaacg
    16141 aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt cacaccagcc
    16201 accgcgttcc acggggactc cacaccggca ccatggctat accccacccc gaaatgcccg
    16261 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
    16321 aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc gacaaattct
    16381 ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag acgcatgttg
    16441 taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga tttcgattcc
    16501 atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc aacatcagcc
    16561 cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat tgtgttcacc
    16621 gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag gcatttttta
    16681 cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata atactgtcgt
    16741 acgcttatct ttcgacaatg aggccgtcgg caaaataaaa tgactaatgt aatcgttgct
    16801 gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc agattatctt
    16861 acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata ttgtcaatca
    16921 atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat tcctatcatc
    16981 acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt taagcagatc
    17041 ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat ctaagttgaa
    17101 gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc cagcgatggc
    17161 ggatatgaat cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt tacttactgg
    17221 caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg gtgcagtaac
    17281 agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtaggtg ccgccgttgg
    17341 tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat
    17401 tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt
    17461 tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg actacaatat
    17521 taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg aaatggcaaa
    17581 aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag
    17641 ccgtgattta aaaattgagc aatctccgga actgagcacc aaagtggtag aaaaactgaa
    17701 tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg acaccatgcg
    17761 tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga cgcggttttt
    17821 tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg tgtcattgta
    17881 gccattatca cccagctgca cgcccacatt cccccagacg ctggccgctg gattcaggtt
    17941 gccatttacg ccaagtttaa tttcccccaa attactcgcc ccatcttgtt cgactttcac
    18001 accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt caacaaaagg
    18061 ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa aggtgcggaa
    18121 accaagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc cattggcttc
    18181 ggtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct ggaatatcca
    18241 cgtgtatttc gccgcttcca gaccaaagga tttattcagg gcaaagccgt aacccgcttc
    18301 cagcgagacg gttgcgcctt tcgcactata ggattcatag gctaattcat cccctttcac
    18361 cgagttttta aaccagctat attgcgccca ggcgtcaatg tatgcgcctt ttttactgat
    18421 gtcatcggca aaccaggtgg catacagccc tgcgcaatag cctctgacac tccctttcga
    18481 acgataatcc gacacgctgg aatgagttga gttgtagtcg cgggcataac cagccatcac
    18541 tcccaaacgc caactgtcac tgtcggtaaa accaccggtt aacagatcgg cccctaactg
    18601 cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg
    18661 cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta caggatcgat
    18721 gtagcgcatt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt tagcggctgc
    18781 caggttggcg acgtaacttc cagcttcgag gcgcaggacg ggggtaggcg caacagtagg
    18841 cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt tagtcagata
    18901 ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt aggcaccggc
    18961 gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc tgataatctc
    19021 tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt
    19081 tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat cacccaattc
    19141 agttctgaga tgcaatacgc cattattacc aacgtaattt tcagttattg ttaatcttgt
    19201 tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat caaccgtgag
    19261 gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa gcgacagata
    19321 ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg aattagttgc
    19381 atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg atgatacctc
    19441 actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt catttgctga
    19501 ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgaccattta tggaagactc
    19561 cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc
    19621 gccattataa attttagcag tcgcggcgtc aggacccgtt gtataaattt ctccctggtt
    19681 aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat gcgtggtgat
    19741 atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac cagccttgac
    19801 gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga tctccccttt
    19861 tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg tagtactttc
    19921 ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg caagaacgcc
    19981 gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa aagagggggt
    20041 aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc cactcgtttg
    20101 acttagcaaa ccaattgagt aatcccccgt tatgacaatc gtgtttttac cctgcaaaat
    20161 aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgtgc ttagaggact
    20221 attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa tggttaactt
    20281 gctaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat aactagcgca
    20341 ttatcaatat cttttacttt tccatctttt attgccacag catgtttata ttcatcgtgc
    20401 tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc caaactaaac
    20461 ccggagagtt ttttcagttt catagattaa ccatccattc attggattaa caaacattcc
    20521 tggtcaaata acctatgcgt tcagattacc aaaacggagg gtaaagacaa aaacaaaagc
    20581 acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag tcggcattat
    20641 catttttcac cttattttca tgacattgat cactttaggg tgacgatttt gtttaacaaa
    20701 taactcttcg acatcaaaat tgttaatagt tgcgatgagg attatttcgg tgatatataa
    20761 aaacaaccgt gataacacca gggttgacaa ctatttgcgc aataatgcct ttccttcaat
    20821 gatccaggcg ttcactgcca gcaacccatt cagccgcata ctgacagtga gaaactgaaa
    20881 gattattgct gatccctgtg gttgacgagt ctttctctta atcgattaac cagattgatc
    20941 gccagccaaa caagggcatt aagcaaattt atcgtcacaa gcacttctcc cagcccggta
    21001 ctgggttttg caataaggat gaccaggagg gtgatcagca taaatgcaat ggaaaagatg
    21061 atatagagag tgaaataatc cagattatta ctattgttca tgctactaat ggcaatcctg
    21121 tcaaaacttt caaacaatta tagcaaacaa aataatgaac aatagtaaca agaggcaacg
    21181 agctaattag ctgtctataa ttacaccgca ctatggcctt actgatcaat tttcactcaa
    21241 ccacaacctt caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag
    21301 gtttatagaa ataataaccc tgctgaaatg tgatattatt ttggttcaga tagtcaagtt
    21361 gttctttcgt ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca
    21421 atacacaatc cagaattcgg gttgattctg ggtctgcgtt tacgcggcca acgaaactgt
    21481 gatcaatttt gatataatca atatgcaggt catgaagata agaaagccct gagtagccag
    21541 taccgaaatc atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa
    21601 ccagactttc atcaacattc aatggctcac gctcggtgac ttccagaaca aggtttaaat
    21661 cgcggcgggt aaaactgtca cggaaattta aaca
//
LOCUS       DAHBHC010000083        21217 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHBHC010000083 DAHBHC010000000
VERSION     DAHBHC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.083"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            99..1184
                     /gene="hcxB"
                     /locus_tag="M2A35_003870"
     CDS             99..1184
                     /gene="hcxB"
                     /locus_tag="M2A35_003870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCB7200112.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(1413..1673)
                     /locus_tag="M2A35_003871"
     CDS             complement(1413..1673)
                     /locus_tag="M2A35_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7200113.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(1938..2204)
                     /gene="ybiI"
                     /locus_tag="M2A35_003872"
     CDS             complement(1938..2204)
                     /gene="ybiI"
                     /locus_tag="M2A35_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCB7200114.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(2278..2955)
                     /gene="ybiX"
                     /locus_tag="M2A35_003873"
     CDS             complement(2278..2955)
                     /gene="ybiX"
                     /locus_tag="M2A35_003873"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCB7200115.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(2997..5279)
                     /locus_tag="M2A35_003874"
     CDS             complement(2997..5279)
                     /locus_tag="M2A35_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCB7200116.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQSRNDSGIDASASIGSTWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(5543..5803)
                     /locus_tag="M2A35_003875"
     CDS             complement(5543..5803)
                     /locus_tag="M2A35_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7200117.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            6079..7005
                     /gene="rlmF"
                     /locus_tag="M2A35_003876"
     CDS             6079..7005
                     /gene="rlmF"
                     /locus_tag="M2A35_003876"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCB7200118.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(7002..9227)
                     /gene="ybiO"
                     /locus_tag="M2A35_003877"
     CDS             complement(7002..9227)
                     /gene="ybiO"
                     /locus_tag="M2A35_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCB7200119.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(9344..10066)
                     /gene="glnQ"
                     /locus_tag="M2A35_003878"
     CDS             complement(9344..10066)
                     /gene="glnQ"
                     /locus_tag="M2A35_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCB7200120.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(10063..10722)
                     /gene="glnP"
                     /locus_tag="M2A35_003879"
     CDS             complement(10063..10722)
                     /gene="glnP"
                     /locus_tag="M2A35_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCB7200121.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(10861..11607)
                     /gene="glnH"
                     /locus_tag="M2A35_003880"
     CDS             complement(10861..11607)
                     /gene="glnH"
                     /locus_tag="M2A35_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCB7200122.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(12011..12514)
                     /gene="dps"
                     /locus_tag="M2A35_003881"
                     /gene_synonym="pexB"
     CDS             complement(12011..12514)
                     /gene="dps"
                     /locus_tag="M2A35_003881"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCB7200123.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(12813..13700)
                     /gene="rhtA"
                     /locus_tag="M2A35_003882"
     CDS             complement(12813..13700)
                     /gene="rhtA"
                     /locus_tag="M2A35_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCB7200124.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            13935..14000
                     /locus_tag="M2A35_003883"
     CDS             13935..14000
                     /locus_tag="M2A35_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200125.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            14053..14568
                     /gene="ompX"
                     /locus_tag="M2A35_003884"
     CDS             14053..14568
                     /gene="ompX"
                     /locus_tag="M2A35_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCB7200126.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(14617..16200)
                     /gene="opgE"
                     /locus_tag="M2A35_003885"
     CDS             complement(14617..16200)
                     /gene="opgE"
                     /locus_tag="M2A35_003885"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCB7200127.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(16472..16600)
                     /gene="mntS"
                     /locus_tag="M2A35_003886"
     CDS             complement(16472..16600)
                     /gene="mntS"
                     /locus_tag="M2A35_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCB7200128.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            16786..17253
                     /gene="mntR"
                     /locus_tag="M2A35_003887"
     CDS             16786..17253
                     /gene="mntR"
                     /locus_tag="M2A35_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCB7200129.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            17250..18368
                     /locus_tag="M2A35_003888"
     CDS             17250..18368
                     /locus_tag="M2A35_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCB7200130.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(18427..19347)
                     /gene="ldtB"
                     /locus_tag="M2A35_003889"
     CDS             complement(18427..19347)
                     /gene="ldtB"
                     /locus_tag="M2A35_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCB7200131.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            19566..21158
                     /locus_tag="M2A35_003890"
     CDS             19566..21158
                     /locus_tag="M2A35_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCB7200132.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT         5186 a         5213 c         5605 g         5213 t
ORIGIN      
        1 aacgggcttg taacccccgg agttatcagg ctgcatggtt cccgtcagag ccttcggggg
       61 aagagtaaac cacaaaataa acacaccagg agttaaccat ggaaagtggt catcgctttg
      121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
      181 aagaagcgaa attagtggcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc
      241 atggtattgg catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta
      301 accatcatgc caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg
      361 cattcggtca ggtcgcggca cacgaagcga tggcgctggg gattgagaaa gcgcatcagc
      421 acggtattgc tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
      481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
      541 caatggtcgc accgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
      601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
      661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
      721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
      781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
      841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
      901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
      961 gcgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gatgataagc
     1021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga
     1081 ttccattgga tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt
     1141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
     1201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
     1261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
     1321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
     1381 atgcggcgta aacgccttat ccttcctacg aattacttat aaattaccgc agtaccgctt
     1441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
     1501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
     1561 ccgattttat tcatgttctg tgcctgggat gccgttaccg gttccgcggc aaacacgcca
     1621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
     1681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
     1741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
     1801 tctaacatac tgataaacca tttatttgtt gtacttatgg acttgtttta tgcagcgtgg
     1861 cagtcacgac agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctata
     1921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
     1981 gtatacgctg gtttttgtaa atctttctcc tgctgacaat gaatgcataa gcgcacgcca
     2041 ggaatggctt cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatccagg
     2101 ctttcgccac gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
     2161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgtc ctccccatat
     2221 caatgcttgt acagttaagt gtagctaatc cagggacgaa cttgggcagt tcaagcatca
     2281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
     2341 attttcacca tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
     2401 gcgcttttta tcatcacgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
     2461 gcgggttacg ggtgtcacgc aatgcaggct gctggaggga tacaacacga gatcgcctgc
     2521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
     2581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
     2641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
     2701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaata aagcatgttg
     2761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
     2821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
     2881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
     2941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
     3001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
     3061 ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag
     3121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
     3181 aacgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
     3241 cccgcgccaa cagagatatc atctgttgcc tgatactggc tccataaggt gaaggcgtgc
     3301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
     3361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggcgtgat attcccggcc
     3421 acggatatct catagccttc gacacgtttc ttaccgtatt gcgagtaagt tccgtcatca
     3481 ttttgctcaa cttcattttc gatatcagta cggaatagcg cggcggtgag caacaggcgt
     3541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
     3601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
     3661 ggaggctgct gggaaacggc atagttaata tagacattac cgttttccgt caggtgatac
     3721 agcgccccgg ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgacc
     3781 ggagaacctt ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag
     3841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
     3901 aaatcacggg tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca
     3961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
     4021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
     4081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
     4141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
     4201 gtccagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
     4261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
     4321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
     4381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag agccgctgtt
     4441 cccgcagatg gggcagaata acccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
     4501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
     4561 gcgatagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
     4621 gttttttcgc ccatcacatt caggcgcacc gcagtggtat caccaatgac ctgattgacg
     4681 tccagcgtgc cgcggcggaa ccaggtgctg ccaatactgg cggaggcgtc aatgccggaa
     4741 tcattgcgcg actgcttgct gatcatattg atcgagcccg tcggcgcgct gcgcccgtag
     4801 tcggtgccgg aaggcccttt aatcacttcg acctgctcag tattgaaggt gtcgcgcgag
     4861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc agcaccgcgc
     4921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
     4981 ccggggacgt ttttgagcgc gtcggtaagg ttggttgcgc cctgatcttt aatcacttgt
     5041 tcagaaatca ccgtcatcgt gcgggtagta tccgctaccg gacgcgagaa tttcggatcg
     5101 gcagattgtt gtggcgcata aagcgaaggc gttgatgctt cgacaaccag cgtgtcatcc
     5161 gcgttagttt gcccttcggc ggcgagtgcc tgagccacag gcgtgatgcc aatacaaaga
     5221 ccggcaaaga acgtgagcga atgaaattgt ctggcaggga aattgcgatt gttttccatt
     5281 ttgcaggtga ctttttttat atgttgaaat tttcactgcc acgagaaagc ctctctgcgg
     5341 atgcgatgcc actgttaatc tcgtgatagt gttcgtgcaa gtgataataa ttttgataag
     5401 cattatcact tggcgcgatt ttctatcacc cattgcgcga aaaagaaata catttattta
     5461 cattgcgttc atatttgatg ttttttgatt acaaaacagg cgaaaactgc atccagaggc
     5521 gaatgcagtt acgttggtgg ggttatttgt agatggttgc agtaccgaac atctggtcat
     5581 ttccgcctgc ggaattaatg acataacctt tcgcgccttg ctcgcgcgct ttttctgcca
     5641 gtttatcctc cagatcgctt aggtttgatg cgccagttgc cgaaacggta ccggcgggcc
     5701 gtagctgacc gctgtccagg ttactcatcg gttgcgcggc ataagccgtg agagagattc
     5761 cgctcaggac ggtggcaatc agtagtgtga ggcacttttt cataatgaca tcctcataaa
     5821 tacaacgggt agtgcctgat aagtgtaggt cgcctgggcg atgaaaatga cgcaaaaaat
     5881 tgacagagat gtgcttattt tttgaacgat atatttttac aaaataagac aaatcaatga
     5941 catgcataat gctgattttt atgatgaatt gccataaacc atagccatgt taaggttttg
     6001 gggaatgata aacgcagcca gatatttacc ctgtcgcgtt atgcgggtat cttacgccgc
     6061 tgcttaaagg agaatgctat gtccgcccag aaaccggggt tgcatccgcg caaccgtcat
     6121 cacagccgct acgatctcgc cacgctttgt caggtcaatc ctgaactcag gcaattcctc
     6181 acgcttacac ccgcagggga gcaaagcgta gactttgcca atccgctggc ggtgaaggcg
     6241 ctcaataagg cattgctggc ccatttttac gccgtagcga actgggatat cccggacggt
     6301 tttctctgcc cgccagtacc gggccgggcg gattatattc atcaccttgc cgatttactg
     6361 gcagaagcaa gcggaacaat tccagctaat gccagcattc tggatatcgg cgttggtgcg
     6421 aactgtattt atccgctgat tggcgtacat gaatatggct ggcgttttac cggtagtgaa
     6481 accagcagtc aggcgttaag tagtgcgcag gcgatcatca gtgctaatcc ggggcttaac
     6541 cgcgccattc gtctgcgtcg gcaaaaagag agtggggcga tttttaacgg catcatccat
     6601 aaaaacgagc aatacgacgc gaccttgtgt aacccgccat tccacgattc cgccgctgcg
     6661 gcacgggcag gtagtgagcg taaacgccgt aacctggggc tgaacaaaga cgatgcactg
     6721 aactttggcg gccagcaaca ggagttgtgg tgtgaaggtg gtgaagtcgc ctttatcaaa
     6781 aagatgattg aagagagcaa aggcttcgcg aagcaggtga tgtggtttac ctcgctggtt
     6841 tctcgtggtg aaaacttacc gccgttgtat cgtgctctga cggacgtggg cgcggtgaag
     6901 gtggttaaaa aagagatggc ccaggggcaa aagcagagtc gctttattgc ctggaccttt
     6961 atgaacgacg agcagcgccg ccgttttgtt aatcgccaac gttaaagcgt tggctccccc
     7021 ggcggcagcg gttcagctgg ggccgcgccc ggagcaggca gcacctgata agtctgcact
     7081 ggcgcgcgaa cacctgccag gtcgaaatgc tttttcacct ggctgtcgag ggcaaagcgg
     7141 accgtccact gtttgagtgg cagcgtggtg aacgaaacac gcagtgtaaa cgcggtattg
     7201 cttaagccga caatcccggc aaagtttggt tcaccaataa ccagcccgcg aatttcttcg
     7261 ttttccatca attccgctac cgcatctttc agtgcctgat tggctttatc agcatcttca
     7321 tggcgatcaa catcgtagtt tgccaccacc gaaccaatgc cgcggacgaa gttagcaaag
     7381 gtggttatcg aagaccaagg aatgatgtga tacgccccgg tatcctgacg cacgcccacg
     7441 gagcgaatcg acatccgttc cacagtgccg gtcaacggcc cgatagtaac caaatctcca
     7501 gtgttcatgc cgttttcaaa ctgaataaat accccggtga taatatcttt caccagcgtt
     7561 tgcgaaccaa acgagatagc cagacctaat gccccagcac ctgccagcaa tggcgcgata
     7621 ttgacgccga tttccgacaa cacaatcatg atggtgatgg tactgataat caccgccagc
     7681 gcgttacgga acagcgtcag cagggtacgc gtacgggcgc tgggtagcgg gcggccatga
     7741 atatccgaag ccagccggtt ttcgatcaaa ctggcgagca ctgtccagcc aaccgccgag
     7801 aagaaaagaa tgagtgcgat gcggatcagg atatctacgg ttttctgccc cgcgccgttt
     7861 tgcagccagt tccagaaatc gaacaatccc catgcgctca acagcaacat taccgccacg
     7921 cagacggtca gaatacgcgc cgttttcagc gccgccgaaa gccagccatt cagccgtttt
     7981 tgtagctccg gatagttacg ctgagtatgt ggcgagaggg tgatggtttt cgccagccag
     8041 cgggaaaaca taccggaaac aaacgctgcg ataccaataa tcgccaggct gcgcaccgtt
     8101 gcacccatca taaatttcag gctgttgccc ggatcgaaca acgaaaagaa aaacagcacg
     8161 ataaaatagg cgcttgccag ccagtgccac accagcgcaa aggcgcggat aaacaggctg
     8221 aaaaaggcca gcgaatgctc cgcaaagttg agcaaatgtt gggtaatctc ttttttattg
     8281 cgaaagatca ggtacaacgc ccagacggtc atgcacagca taatgatgac gttcgccagc
     8341 gcacctatct gtacattcac ctgattagag ataatcggca cggccacaat caggccataa
     8401 cctatcaaac tgcttaacca gctcaggcga cgactccagt aacgggcgct ctcgtcctga
     8461 atcgtgaacg ggcgcagttc agccacgttt gggcaaaaaa tcaggcgtag tacggctttg
     8521 aaaaattcaa tcagggcaaa ggcgttgaga aacaaacttt gttggaaagc gatggtgcga
     8581 ctgcccgcat tcaggttgtc gcttaatacc tggccgacaa acaatgtcag tgccagtaac
     8641 agtaggtcga taataaacgc cccgataatc atcgccggaa gctgcagcca gttgctgcgt
     8701 tcgcgatttt tttgccgcgc ccactggccc attttgcgat acagcggcag tgcgcacaga
     8761 cgaatcagcc agtaaaaacc aaacactaat accgctaaca ttgaaaaatg ggtcaacgca
     8821 ttgctgaagg tttgtggatt aaacggctta tgcggggagc cggtgatatt gcgataaagt
     8881 tgtccgaagc gggcggaaag ggcttcacca taatggcggc tgacttcggt gaccttttgc
     8941 agcacggttt gctcttcaac cagcgtcggc ggcacgatct tcgggaccgg ttccgcaggg
     9001 ggcgtcgcgg cgacagtacg caactggtcg atcaattctt tacgcgaggt gtcgttatcc
     9061 agcacatccg ccagtgcgcc ataggccgct tttttttgtt cgatatccgg ttccggggcc
     9121 ggttcagtcg ttgagtccgt tgtcgttgtg gttgtaacgc cgggtatgga taccgcgtgg
     9181 gcaggtgcgc ccagcaggca gaagaggatg aacaggatcc accgcatgac tcctccagtg
     9241 agaaaacaga gcaaaaagat aagtatagat gctgggggag gggcgatttt aaatgagagg
     9301 aatctggtgt gcctcccttt cgggtgaaag ggaggcagag aagttaagag acgtgctgca
     9361 aaaattcctg caagcgctgg ctcggcgggt tcttgatcaa tacctgcgga tcgccatctt
     9421 ccgcaatccg gcctttgtcg ataaagatca gccgcgaagc tactttctcg gcaaaaccga
     9481 tttcgtgggt cacgatcacc atcgtcatcc cttcttcagc cagatcctgc ataaccttca
     9541 gcacttcatg gcgcagttcc gggtcaagag cggaagtcgg ttcatcaaac agcatcattt
     9601 tcggcttcac cgccagcgcg cgggcaatcg ccacacgctg ctgttgacca ccagaaagtt
     9661 cggaagggta gtgatgtgca cgttctgcca gaccgacttt cgccagcagc tcacgcgcca
     9721 gtttttccgc ctcttctttg ttcgcgccac gcacgcgtag cggaccaaac atgacgtttt
     9781 ccagcgccgt cagatgcggg aagaggtaaa actgctggaa caccatacct gcttcctggc
     9841 gaatcaggcg ctcgtcaact ttcggatcgt taaccttcag gccatcgaca atcagatcgc
     9901 cggaggtgat ttcttccagt ttgttgatgc agcgcagcag ggtcgattta ccggaaccgg
     9961 acggcccgat aatcaccacg acttcgccct gggcaatgtt caaatcgata ttgtgcagca
    10021 cctgggttgg gccaaagtgc ttggagacgt ttttaaattc aatcacagga ttttcatcct
    10081 tctttccaga cgacgcagaa taaagctcag caccagggta ataatcagat agaacaccgc
    10141 cacggcgctc cagatctcaa gggcgcggaa gttaccggca ataatttctt gcccctgacg
    10201 ggtcagttcc gccacgccga tcacaataaa cagcgaggtg tctttaatgc tgatgatcca
    10261 ctggttaccc agcggcggca gcatacgacg cagtgccagc ggtaaaatga cgtagcgaat
    10321 ggtttcccaa cgtgaaagac cgagcgccag tcctgcttca cgaaaacctt tgtggataga
    10381 cagcaccgca ccacgcgtga tttccgcaat gtacgcgccg gagttgatca tgatggtgac
    10441 caccgccgca gtaaatgggt cgatgcgtaa gtcgttaaac gccatcggca gggcgaaata
    10501 gataaacatc acctggacga cgataggtgt gccgcggatc acttcaataa agaccagcgc
    10561 gacgtggttg gctatccaac caccgaaggt gcgtgcaaaa cctgccagca atccgattac
    10621 cagaccgcct gccagaccga ggaccgaaat ccacagggtc attttggcac cttcaatcag
    10681 aagcggaatg gcaggccaga tggcactcca gtcaaactgc atatgttgtt cctgttaccg
    10741 tggtgtaaat aatcaaattc agggtaatta atggccccgg agcgggtttg cgcccctcac
    10801 cctaatcctc tccccatagg ggagagggaa ctgccagtgc gttttacagg tgtagcgtta
    10861 ttatttcggt tcagtaccga accatttttt gtagatttcg ttgtaagttc cgttctcgcg
    10921 cagggttttc aacgcgccgt tgactttgtc acgcagctcg tcgctacctt tcgggaacgc
    10981 aataccgtat tgctgcgctt ccagagagtc acctaccgct ttgaactgac cgttaccggc
    11041 ggttttgatg aagtacagaa tgtttggcgt atcgtgcaga acggcgtctg cgcggttggt
    11101 gcccagttcc atataggcgt tatcgatgtt cgggaactga cgcagatctt tagttttgat
    11161 gtttgctttc gcgtaatcaa cggagccagt accgctcttc acagcaacca ctttcccgtc
    11221 gagatctttc acgcttttca catcgttatt gttagctttc accatcacta acaggccgct
    11281 tttgtagtag ccgtcagaga aatcgatcgc ttttttacgc tcgtcggtga tggtaatgcc
    11341 cgccagcgcc agatcgacgt ttttggtttg cagtgccgga atgatcccac tgaaatccat
    11401 cggcttcagt tcgtaatcca gcttcagctc tttagcgatg gcagcccaca gatcaacgtc
    11461 aaagcccaca tatttatcgc cctgtttaaa ttcaaacgga acgaaggcgg tatccgtcgc
    11521 gacaactaat tttttatccg cggcatgaga agaaaccgca aaagccaggg tcagtgcagc
    11581 cagtgaaact tttaatacag acttcatagc atttcctttt ttctatccac gggacgatcc
    11641 cctgcgtgac attcacatat atgaaaaaat cgtgccagtt ttgcaactcc ttgatttttc
    11701 gagatgcggc aaaaacgtga tgcacgattt atagggcaat accctgaaga tgcaccattc
    11761 tggggcacca atctggtgcg ctaaaattgt gcactcaaca cagtgattat ttagcgtaaa
    11821 acctgatgaa aaaacaatct ttatgtaacg attgtgtgat gatgtggata caaaaaattt
    11881 acttaatcag ctggagatag cagatggatg cactaaataa gtgcgttgag gtgggctgca
    11941 aaacaaaacg gcctcctgtc aggaagccgc ttttatcggg tactaaagtt ctgcaccatc
    12001 agcgatggat ttattcgatg ttagactcga taaaccacag gaatttatcc aggtcgcgag
    12061 acgcggcggt caggatatct gcggtgtcgt catctttcgc ttcgccaatc gctttgcgta
    12121 cgtcattagc gacgattgcg taacggtcag ccagttcttt caggtgatcc tgaacgttgt
    12181 ggatgtccag cgggtaactt ttcagcgggg ttttgctgtt gataacttga gtggtcccca
    12241 gagctacacc gcccagctgc actgcacgtt ctgccatggt atccagatga tcgatcagtg
    12301 cggtgcggaa gccatccagc atttcatgta cggcaatgaa gttagcgccg cgcatgttcc
    12361 agtgcgcttg tttggtaatc aaagaaagat caataaactg gataacctgg cgattcagca
    12421 actctactgt tgcttttttc tcgctgtcgg agacatcgtt gcgggtataa agcagattgg
    12481 tcgcttttga tttaactaat ttagcggtac tcataatttc atatcctctt gatgttatgt
    12541 cccagtaatt aacgagacta agtatagcac cggctatgtg ttccgctatt ctggctgttc
    12601 ctatcacact aatagtggta acaagcgtga aaaacaaaac taataaagat tcaatgagtt
    12661 agatatattg ataagaacaa ttcttattta tctgctcgcc aggaattttc ggaataaata
    12721 tgcttttgca taattcgata tataaagacg gtgtagagga aaagtagcga gaaattctgc
    12781 atggttatgc ataaccatgc agaaatgtaa atttaattaa tgtctaattc ttttattttg
    12841 ctctctttgc gtactgtcag cgtagacccc attgaagcgg cgatgatagc gccgagcgcc
    12901 agcagctgta tgggtgtcag tgtttctccg aggaaaatca tcccggaaac ggcagccagc
    12961 gccggttcca tgctcatcag cgtaccaaat gtccgtgttg gcaaacgggt gagggcaatc
    13021 atttccagcg aataaggcag agcggtcgag agaatagcga cagccagacc caatggaata
    13081 accgaccagt gccagagtgc ttcaccagcc tgaagcgctc caattggcac gaaaattaac
    13141 gctgcaatca acgaaccaat tgccaccgtc gcagggccat gttccgctcc tgcgcgttgc
    13201 ccacttaaaa tgtaaatagc ccaacaagcc ccggccccca gtgccagcgc acagccggtt
    13261 aaatcgacat gggaaacgtc ttgccccagc ggtagcagga accacagacc aagaaccgcc
    13321 agcacaaccc agacgaaatc taccgggcga cgagaagaga acagcgccac cgccagtggt
    13381 ccggtgaact ccagcgccac cgcaataccc agcggtactg tctgaataga aagataaaaa
    13441 agataattca tcccacccag cgaaacgccg taaaacaaca gcggtaaccg ttgctctttg
    13501 gcaaagcgca gtcgccatgg cttaaagaac gcgatgagga tcagcgttcc taatgccaga
    13561 cgcagcgcag tgacacccgg tgcgcccacc agaggaaaaa gtgacttagc taacgaggct
    13621 ccaccctgaa tagacgccat ggcaacgagc aatatgatta ttggtaacca gaccggcatt
    13681 ttacgtaatg aaccaggcat cctttctccc acaaatatct agacttaagt aaagcgtgga
    13741 gtgtactgga tatacccaat gctggttgag catttgttga aaaaattttc ccccgttttg
    13801 actaaaatgc gccaggattg atggaatcat tagtctggtg attaggaata atctggatga
    13861 atgacaggga aaacatgcgt aatacttacg cagttctctg aaaaagtgat ttaaatttag
    13921 atggatagcg gtgtatggaa acgttctgtt acatgaaatg gcccgttaga catcacaaat
    13981 cgcgaagagt ttcccattaa tttttgatat atttaaaact taggacttat ttgaatcaca
    14041 tttgaggtgg ttatgaaaaa aattgcatgt ctttcagcac tggccgcagt tctggctttc
    14101 accgcaggta cttccgtagc tgcgacttct accgtaactg gcggttacgc acagagcgac
    14161 gctcagggcc aaatgaacaa aatgggcggt ttcaacctga aataccgcta tgaagaagac
    14221 aacagcccgc tgggtgtgat cggttctttc acttacaccg agaaaagccg tactgcaagc
    14281 tctggtgact acaacaaaaa ccagtactac ggcatcactg ctggtccggc ttaccgcatt
    14341 aacgactggg caagcatcta cggtgtagtg ggtgtgggtt atggtaaatt ccagaccact
    14401 gaatacccga cctacaaaca cgacaccagc gactacggtt tctcctacgg tgctggtctg
    14461 cagttcaacc cgatggaaaa cgttgctctg gacttctctt acgagcagag ccgtattcgt
    14521 agcgttgacg taggcacctg gattgccggt gttggttacc gcttctaatc actttggtga
    14581 tataaaaaat ccgcctctcg gggcggattt ttgtttttaa ggcttcgggt cgaaaatatc
    14641 ggttccgaga tggttgtaat ccaccttctg taactggaag ttggtgatgt atatcgaccc
    14701 ggctttcttc tcagatataa acgggtattt gatgttaatt tccttcgctt taattcccgt
    14761 ccactgggag aaaaaaccta agaagtcatt ggctgagcgg cgggctttaa tcacccgatg
    14821 cgctttatcg tcgctggaaa tgaccataaa aggcacctgg aaattttgct gatacttatc
    14881 atcatgggca aggtattgca cgtctttgcc gcgctcttta aaggccagac cgtggtcaga
    14941 aaagtaaacc agcgagaagc tgctgccgct gttgcgtaac tgatcgtaca gcttgcgcag
    15001 taaatcgtcc gtttgcgtca tggtatagag atagcacgac gtttctttcg actgcacaaa
    15061 ggtttcgtat ttgccttgtg tcctgtcgca ggcctgcgga tgtgagccca tcagatgtag
    15121 aacaatcagc tgcggttgcg agtgctcttg cgccagcact tgagcggtca tatccagtaa
    15181 cgcttcgtct ttggtatttt tatctgcttc aaaattacct tctttcagga agtacacttc
    15241 atctgctcgt ttggcgatgc tggcgatagc ggtatcgtat tcgccgattt gaccctggtt
    15301 ggaaaaccac caggtctgga agcccgcgcg atttgccagg gtgacaaagt tatcctgaaa
    15361 ctgtggtttg ccatcgacta cgcgattgag cgttaagcca agcgatttct gcgtggagcc
    15421 actggcggca atgtagtcag caaatatcaa accgttaacg ctgctggcaa acggggtatt
    15481 gtcccagtga ccgccaaagg caccgagggc atcgcgacgc gcgctttcac cgatcaccac
    15541 cacataggtc tgatacttcg gcttaacggc agtgaccgtc caggtatctt tcattccgga
    15601 aagttttgcc atccgttctt gctcttcaat cacctcgtta ttattgacga tgacatcctt
    15661 agcaaaacga aataccggat agccagtatc tttcagttta aatacgccgc cccaggccag
    15721 gttttgcacg ggagcaacaa aaaatgtcgc cacactgaat accagacaca ggctgtcgaa
    15781 ggtattccag cgcgcttttt cactctcttt ttttcgcctg attgctatta cgccgagcac
    15841 aaaaataaat atgccgacca tgtagctgta ccacggaaaa atcgtcagga tttcggtcga
    15901 ctcttccata ttggtggagt gcaatgccag caatgtattg aaattaggcg cgccgtaggc
    15961 ctgagcaaaa gggaaataac aagccgccac cagcgaactg acaccgacca gaactttttg
    16021 tacgcgaggc aatgttcgcc atagcaaaag caaaatagcc gtaaacgcag cggtgtagag
    16081 caaactgaag gggtaaccta agccgaggtt aattaatagc gactgtaaaa agtagaacgc
    16141 agtccacggg ctaaataccc ggctacgggt aacaagcgat tctttgaggg ttaaattcat
    16201 atgccactat cgtaacaaac gccatgtgct taccctggcg tcaagggtca atacctgcaa
    16261 gagtgcgtga agagtggaaa ggggtccgca tccgcgagcc gcaatatacg cagggctgca
    16321 agaagataga gcgagccgcc gttcaggtca actactgacg aacaaatctt ttgcgaaggg
    16381 gattgcaaat ccgacaaaaa gtggggtttt tcagaccaaa acgcgttatg cctgcatgaa
    16441 agcaggagtg aagcggcgta ccgcgacgcc gctatttatc ggaaggttta tcttgctgcg
    16501 gtttgttgtt gaccatatcg cacaacatag agagcagcat taaccgtact ttaaagggag
    16561 aatgactaaa cacgcgcata cacctcttga actcattcat aagacctcct gacttgctaa
    16621 tcccgtcgat ccttgaggga tgattgcatt acatacagat atagcacagg ctatattata
    16681 tagctattgc taaaacgtta attttttgta ccttcgcaac tctggtttac aatgtgcgca
    16741 cgaaatgaga acgctatgta tgcgtcacca taatgaggaa gcacaatgag tcgtcgcgca
    16801 ggtacgccaa cagcaaaaaa agtgacgcag ttagtgaacg tggaagagca cgttgaaggg
    16861 ttccgccagg tcagagaggc gcatcggcgc gagcttattg atgattacgt tgagctgatt
    16921 tctgacttga tcagggaagt gggggaagct cgtcaggtgg acatggctgc tcgtctggga
    16981 gtttcgcaac cgacggtggc taaaatgctt aagcggctgg caaccatggg gctgattgaa
    17041 atgatcccct ggcgtggcgt gtttttaacg gcagaaggag agaagctggc gcaggaaagc
    17101 cgcgagcgac atcagatagt cgaaaatttc ttgttggtgt tgggcgtcag tccggaaatc
    17161 gcccgtcgcg acgcggaagg catggagcac catgttagtg aagagacgct cgacgctttt
    17221 cgtttgttta cccagaaaca cggtgccaaa tgagcctgcc ttttttacgc acgctgcaag
    17281 gcgatcgttt ttttcagtta ttaattcttg ttggtatcgg attaagcttt ttcgtgccct
    17341 ttgcaccgaa atcctggcct gctgctatcg actggcacac catcatcacc ttaagcggcc
    17401 tgatgctgct gaccaaaggt gtggagttaa gcggttattt tgatgtgctg gggcgcaaaa
    17461 tggtgcgccg ctttgctacg gagcgtcggc tggcgatgtt tatggtgctg gcggcggcgc
    17521 tgctttctac ctttctgacc aacgatgtcg cgctgtttat tgttgttccg ctgactatca
    17581 cgctaaaaag actgtgtgag atcccggtta atcggctgat tatttttgag gcgctggcag
    17641 tcaacgctgg ttcgctactg acgccaattg gcaacccgca aaatattctt atctggggac
    17701 gttctggtct ttcgtttgcc ggatttattg cccaaatggc accgctggct ggcgcaatga
    17761 tgctgacgct cctgctcctg tgctggtgtt gtttccctgg aaaggcgctg caataccata
    17821 cgggggtgca aacaccggag tggaaaccgc ggctggtgtg gagttgtctg gggctgtata
    17881 tcgtctttct gacggcgctg gagttcaaac aagagctgtg gggactggtg attgtggcgg
    17941 caggctttgc gctgctggca cgtcgcgtgg tgctcagtgt ggactggacg ctgctgctgg
    18001 tgtttatggc gatgtttatc gacgtccatt tactgaccca gcttccagcg ttgcaaggcg
    18061 tgttgggtaa cgtgagtcat ctatctgaac ccgggttatg gttaacggca atcggtttat
    18121 cgcaggtgat cagtaacgtg ccgagtacca tattgttgct gaactatgtg ccgccgtctt
    18181 tattactggt atgggcggta aacgtaggtg gctttgggtt attacccgga tcgctggcaa
    18241 atttgattgc gctacgtatg gcgaacgatc gccgcatctg gtggcgtttc catctctatt
    18301 caataccgat gctgttgtgg gcggcgttgg tgggatatgt tttgttagtt atactcccgg
    18361 ccaactaggg tctggcaaat aaaaaaggcg gattatgagt ccgccttttg ctttaagttt
    18421 tgaagattaa ttcagacgaa ccggcatccc ggagcggttt ttaatcgctt catcaagaac
    18481 aacctggtca acatctggct gaccggtcac tgtttgcacg ctcttcgtca gggtaattgg
    18541 cacaatttcc tgaccttcaa actgggcttc ggtggtagac agcgggttat ggacttcaat
    18601 ataacggctg ccgtctggct cggtggtcgc ttttaccggc tcatcaataa actgtacgcg
    18661 ggtaccgacc ggtactttct cgaacaggaa tttgatgtct tcgttacgca gacgcacaca
    18721 accatgactt acacgcaggc cgataccgaa gttggcgttg gtgccatgga tagcgtacag
    18781 gcgaccgatg tagagtgcat acagccccat cgggttatcc ggacctgccg gaacgacagc
    18841 cggaagcggt tcgcccgcag cgcggtactc tgcgtgcatt ttggcggtcg gcgtccaggt
    18901 cgggcctgct ttcttacgct caactttagt ggtccagttg ataggcgtat ctttgcctaa
    18961 ctgaccaatg ccgatcggca gcacgataac ggtgttggtc cctttcggat agtaatacag
    19021 acgcatctct gcactgttaa tgacgatgcc ttcatgaacg gtatccggca ggatcagctg
    19081 ctgcggaatg ttcagtacag taccgccttt cggcaggaag gtatccacac ccgggttcgc
    19141 ttccatcata ttggaaagcc ccatctggta ctccgcggca aaatactcca gcggctgagt
    19201 gttaccttca ggaattgtga tcacctgatt ctgaccaacc aggcgactcc cgtcggttgg
    19261 cagaggataa gttaccgcag aggcggtact gcaaaagccg acaacagcga aggccgctgc
    19321 gaataatgtt ttcaatttca tattcatgtt aagcgagatt ttgtgcctgg caggccattg
    19381 ggttgagaat attagagtat tggaagcgca ttataagttc attccagctc acagtgaaat
    19441 cagatgtgta cgaaatcaca ttttttgcct ttggcttgag tgtagacctt aagcgaggag
    19501 caggatcttc tttcagactt atggcataat gcgcggtttg tcatatctct tttcaggata
    19561 cgcctgtgtt agtttccagt aacgtcacca tgcagttcgg cagtaagccg ttgtttgaaa
    19621 acatttccgt caaatttggc ggcggcaacc gttacggcct gattggcgcg aacggtagtg
    19681 gtaaatccac ctttatgaag atcctcggcg gcgacttaga gccgactttg ggtaacgttt
    19741 ccctcgatcc caacgagcgc attggtaagc tgcgtcagga tcagtttgcc tttgaagagt
    19801 tcactgtgct ggatacggtg atcatggggc ataaagagtt gtgggaagtg aagcaggagc
    19861 gtgaccgcat ctatgctttg ccggaaatga gtgaggaaga cggctataaa gtggccgatc
    19921 tggaagttaa atacggcgaa atggacggtt actctgcgga agctcgcgcc ggtgaattgt
    19981 tgcttggcgt gggaattcca gtggaacagc actacggccc gatgagtgaa gttgctcctg
    20041 gctggaagct gcgtgtgctt ctggcgcagg cgctgtttgc tgatccggat attctcctgc
    20101 tcgacgaacc gaccaataac ctcgacatcg acaccattcg ctggctggaa caggtgctga
    20161 acgagcgtga cagcaccatg atcatcatct cgcacgaccg tcacttcctt aacatggttt
    20221 gtacccacat ggcggatctg gattacggcg agctgcgcgt ttatccgggc aactacgatg
    20281 agtacatgac ggcggcgacc caggcgcgtg aacgtctgct ggctgataac gccaagaaga
    20341 aagcgcagat tgctgagctg caatctttcg ttagccgctt tagcgccaac gcctcgaaat
    20401 ctcgccaggc aacttcgcgc gcgcgccaga ttgataaaat caaactggaa gaggtgaaag
    20461 cctccagccg tcagaacccg ttcatccgtt ttgaacagga taagaaactg ttccgtaacg
    20521 cgctggaagt ggaaggtctg accaaagggt ttgataacgg tccactgttt aaaaatctca
    20581 acttgctgct ggaggtcggt gaaaaacttg cggtactggg taccaacggc gtcggtaaat
    20641 caacgctgct gaaaacgctg gtgggcgatc tgcaaccgga cagcggcacc gtaaaatggt
    20701 ctgaaaacgc gcgcattggg tactatgctc aggaccacga atatgagttt gaaaatgatc
    20761 tgaccgtgtt cgaatggatg agccagtgga agcaggaagg cgatgacgag caggcggtac
    20821 gcagcattct cggtcgtttg ctgttcagcc aggacgacat caaaaagcca gctaaagtgc
    20881 tttccggtgg ggaaaaaggg cggatgctgt ttggtaagtt aatgatgcag aagccgaaca
    20941 ttctgatcat ggacgaaccg accaaccacc tggatatgga atccatcgag tcactgaaca
    21001 tggcgctgga actgtatcag ggcacgctga tctttgtttc acacgaccgt gagttcgtaa
    21061 gctccctggc gacccgcatt ctggaaatca ccccggaacg cgtgatcgac tttagcggta
    21121 attacgaaga ttacctgcgt agtaaaggga tcgagtaagt gattatccgt caccccgata
    21181 ctgtcggggt gacggcggct gatgacaaat gcaaaat
//
LOCUS       DAHBHC010000084        21216 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHBHC010000084 DAHBHC010000000
VERSION     DAHBHC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.084"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..96)
                     /locus_tag="M2A35_003891"
     CDS             complement(<1..96)
                     /locus_tag="M2A35_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7200133.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPF"
     gene            complement(159..2271)
                     /locus_tag="M2A35_003892"
                     /pseudo
     CDS             complement(159..2271)
                     /locus_tag="M2A35_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            complement(2344..3354)
                     /locus_tag="M2A35_003893"
                     /pseudo
     CDS             complement(2344..3354)
                     /locus_tag="M2A35_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053334.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(3444..4465)
                     /locus_tag="M2A35_003894"
                     /pseudo
     CDS             complement(3444..4465)
                     /locus_tag="M2A35_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4379..5065
                     /locus_tag="M2A35_003895"
     CDS             4379..5065
                     /locus_tag="M2A35_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7200134.1"
                     /translation="MTLVFNHLSDSFKLELRGKGTFSPTSFDHASPHFHCFNINRISR
                     CPELQCHIISQWLQVRLRSPSADSADSPWFFPSRNGPHITRQRFWQIIREYAVMAGLA
                     VRVHPHMLRHACGFALAERGNDTRLIQDYLGHKNIRHTVRYTATSPARFRQAWIHPPC
                     HTLSPDNTSPEHDVMHTGYHIRSSSAHILPPHLSVIHHEYGNMMKNICRELDNIQHIL
                     KRMDARATAG"
     gene            6024..7133
                     /locus_tag="M2A35_003896"
     CDS             6024..7133
                     /locus_tag="M2A35_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCB7200135.1"
                     /translation="MKKLTVTISAVATAVLMAVSAQAAEIYNKDGNKLDLYGKVDGLH
                     YFSSNKDENGDKSYMRMGIKGETQINDDLTGYGHWEYQIQANTPENENHAWTRLAFAG
                     LKVSDYGSLDYGRNYGVVYDVTSWTDVLPEFGGDTYSADNFMQGRGNGFATYRNTDFF
                     GLVDGLNFAVQYQGENGGVNGENTTNNGRGALRQNGNGVGGSITYDYDGFSIGGAVSS
                     SQRTWDQNHTGLMGTGNRAETYTGGLKYDAYNVYLAAQYTQTYNATLAGNLGWANKAQ
                     NLELVAQYQFDFGLRPSLAFLQSKGKKLGSINGRNYDNQDILKYVDVGATYYFNKNMS
                     TYVDYKINLLKDNQFTRDAGINTDDIVAVGLVYQF"
     gene            complement(7293..8060)
                     /locus_tag="M2A35_003897"
     CDS             complement(7293..8060)
                     /locus_tag="M2A35_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200136.1"
                     /translation="MDAVCSVVLVCQPFDLIICKKPVSFRKNTVLLLEPAARSKLSDC
                     PSLVRTVELDHKTVLSFLNDVNNRLPDMFCIDRQGYVIEENIPLSLVYSLFEGIRIAD
                     AYTTSLREKLCLSLLSVFQERTKVISFLLTYMNTFSYKIMGIIGGDLERAWHLKDIAG
                     RLYASESLIKKRLKEEGTSFSEILRDMRMESARKMILENTHSVSMVAQKCGYNSTSYF
                     ISAFKDYYGMTPLHYYDNAVSEMAENKQKAPLRLTGR"
     gene            8962..9555
                     /locus_tag="M2A35_003898"
                     /pseudo
     CDS             8962..9555
                     /locus_tag="M2A35_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517690.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
     gene            10000..11196
                     /locus_tag="M2A35_003899"
     CDS             10000..11196
                     /locus_tag="M2A35_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCB7200137.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLIQIHPALERVLGPRLEHPAILDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVAVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            complement(11222..12112)
                     /locus_tag="M2A35_003900"
                     /pseudo
     CDS             complement(11222..12112)
                     /locus_tag="M2A35_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12185..12367
                     /locus_tag="M2A35_003901"
                     /pseudo
     CDS             12185..12367
                     /locus_tag="M2A35_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(12522..13256)
                     /locus_tag="M2A35_003902"
     CDS             complement(12522..13256)
                     /locus_tag="M2A35_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200138.1"
                     /translation="MIFVCSVVLADRAFTLQFAGKMLSLSAGDVLLIDDTDREDFQPH
                     AAHITEVRLDENIIRRYLESCGGNREADNTVSSGFIRVAFAHPALLRDVMHHLNSGGF
                     VHPGFSEQMFFSCIAVFASKKGFLPLLLGGMLSVAGRVRHIICTDLSRQWKLRDVSSR
                     LYMSESQLKKKLKEEGCSFTGVLLDVRMGYARRLLQARLPVNRVAAQCGYVSTSYFIC
                     VFRECFGVTPHRMLALQEHGQHKSEC"
     gene            13346..13654
                     /locus_tag="M2A35_003903"
     CDS             13346..13654
                     /locus_tag="M2A35_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7200139.1"
                     /translation="MITDKKAEELEQRGLWRRAAARWQELLLNYLQEDDRICIQRRIQ
                     HCLKRAKMKSQRATSLEEFSFRLLRSSAIQTEKEMGIYRDRKTAHDHLYRQDGKKSPH
                     "
     gene            13813..14151
                     /locus_tag="M2A35_003904"
     CDS             13813..14151
                     /locus_tag="M2A35_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type lysozyme inhibitor"
                     /protein_id="HCB7200140.1"
                     /translation="MKKYSSAVLALILTSTCMTASAATQEISKKAYVCDGNKTMEVVY
                     INTTAGNSYAVINQMDEMIPMQLMKIASGANYEAINKNYSYKLYTKGKKADLVEGNDR
                     PVLSNCSSAE"
     gene            complement(14708..14848)
                     /locus_tag="M2A35_003905"
     CDS             complement(14708..14848)
                     /locus_tag="M2A35_003905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200141.1"
                     /translation="MDNEPKNEEKRRDEINLLYVAATRARNTLVLNSVLHQILANRRF
                     TG"
     gene            14931..15987
                     /locus_tag="M2A35_003906"
                     /pseudo
     CDS             14931..15987
                     /locus_tag="M2A35_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16058..16216
                     /locus_tag="M2A35_003907"
                     /pseudo
     CDS             16058..16216
                     /locus_tag="M2A35_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            16278..16523
                     /locus_tag="M2A35_003908"
                     /pseudo
     CDS             16278..16523
                     /locus_tag="M2A35_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            17111..20608
                     /locus_tag="M2A35_003909"
     CDS             17111..20608
                     /locus_tag="M2A35_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7200142.1"
                     /translation="MNKNIRILQFLVSILYSVQSHFSGAQTIQLNGNGIPESITRSIT
                     GVDGNAALNISVPYKTSYTQNILSVESSINIKGGTSNTSIGGAGVYGENFTLNNNGSV
                     WGGDGYNGGVAVSGNKISINNYRNVYGGNGLGGSGSSGGAGLSGDDIIVDNYRSIYGG
                     DDLGGTGGSGVTGNNITVHNSGGIWGGNGVNGGDGINGSNLFITNDNMISGGYGIKQG
                     GDAISGNQITLNNNGIVQGGYGPDGSCSVYGEDIHINNHGNISGSYNSQKDAYNTSII
                     FSGGYNSLDIYSDSVINGDIKLASIPVNGTNELIIKNINNATAINGGLMIGNGSSVYL
                     SGKNIIFNGNISIDEDASMNLSAGNANVHANTITLKSDSWLNIDTSIKNWTQDYYTLL
                     SSDTGISIADNSHIVQYNVLLTEGAESYVYTSLNDDDNKLISMLRWNNTKGMGYGTFN
                     IEKDATLNIGVSLSDNLSPLLYDGWDGKSLTKSGNGTLILSATNNYTGNTEVKSGVLI
                     LAAPDALGRTEYLYLSRGAELDMNGYPQTISKLLTAAGSVLNIHGGSLILNNGGESAG
                     TIAGDGSLNINGGMLDITGNNRYFSGVFTVNKGAQLAVSTADNLGTAFVDNYGTLTLN
                     STSAWQLTNNISGYGNVRKTGAGALNISDNAKWTGMTDIIQGTLILGNADSPVMLGSN
                     QVIVEEQGKLSGFGGVAGNLSNSGIVDLTTYMPGNILTVGGNYTGRNGLILLQTETGG
                     DNSKTDRLVIKGNASGRTRVAVTQAGGTGAETLNGIEVIHVSGNADNAEFIQTERITA
                     GAYDYILKRGQGINSTNWYLISRKDIPVPQPEAVPENHDNNLRPEAGSYVASIAAANN
                     LFVTNLYERQGQELYISHMPGEENEAGIWMYNKGKHNRWRDNSSQLRTRGNSYVVLIG
                     GDIAQWSLNGTDRWHTGMMAGYGHNHNSTNALSTGYHSEGRMNGYTAGLYATWYANDE
                     THNGSYLDSWLQYSWFDNHINGERLPAESWKSKGFTVSLEAGYSWKAGEFTDNYKGSH
                     EWYVQPQLQVVRMNVKSDKYHESNGTSIENTGNGNILTRLGARTWLTSKNGKNTRYAV
                     PFRPFVEAHWLHNSRVFGTSMNGVSIYQDGARDIGEINGGVAGMITPEVAFRADAGIQ
                     LGVHGYHNTSAMLSVEYRF"
     gene            complement(20783..20988)
                     /locus_tag="M2A35_003910"
                     /pseudo
     CDS             complement(20783..20988)
                     /locus_tag="M2A35_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            20978..21181
                     /locus_tag="M2A35_003911"
     CDS             20978..21181
                     /locus_tag="M2A35_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCB7200143.1"
                     /translation="MAFVSDSDDSYCWNEVDKRFSVDDTPNEPNRFGWVGEIDQYGYF
                     SENNENICALYNQIKPRSQHKYY"
BASE COUNT         6120 a         4906 c         4810 g         5380 t
ORIGIN      
        1 aaacggaaaa caggttgcca tgatgaaaaa gtagagaaag aagaacagac cgaaaatcca
       61 gaaatcggga tttttatgcg tacttgcaga attcattatt cttaccctca cgggataata
      121 ttgagaaaca ggacggagct gaggctccgt acctctcttc acacagcttt ttcaagtgct
      181 atcaaaattg cactctccgg atccagtacg gggagctgaa tccctccctg tgccagccat
      241 tcaccactga cctcgagggg ctggttcatc cagactggta atctacgcat ggtatggcca
      301 ccttcaccaa ccagctgaat ttccgggtga tcaataaccc gaagtcggta acgcacttct
      361 gccgccagtc cgggaaaacg aagtatgcca ggtaaggtgt aatccggcat tgcaagctgg
      421 ctgatcataa aaagtgcctg agactgatca gggctgacta ctccctgaac ctgtatcgaa
      481 gaatccggca tatccacacg ccagagaaca cctgtatgaa tcagttgtcg ccattcttta
      541 tagagcaagg cataccgacg ataaccgtca gattccatct catctgcggt cacaggatcc
      601 agctccaacc ccatatggcc gaacaatgcc gtcagaccac gaaaagcgat gctgtgctgc
      661 cggaaagttg catggcagcg acgatagcca atatgagccc ccatcacctc cggagggaaa
      721 aagtaactca tgccacgctg tatggtgcag cgttccagtg catcattatt gtcagatacc
      781 cagaaccggt gtgtgcgttt caggacttcg aagtcaatac gccccccacc ggaagcacag
      841 gattcaaact caacatgtgg aaaacgttca cgaaggagat caagtaatcg gtagaactga
      901 cggggctgtg catctgctgc agcatggcct tcatgccccg cctgtacaag ctcacgattc
      961 atgtcccatt tcacataatc aaccggatgt tctcccagta accataagaa acgttcataa
     1021 atataagcaa aggcctccgg aatattcagg ttaagaacat actgatatct tccggttggc
     1081 tgggaatatc caggcattga caatatccag tccggatgaa gacgaaacag gtcagaatcc
     1141 gggttaatca tttctggttc accccatata ccaaactcca taccgagaga tttcacatga
     1201 ttaataaccg gcatcagccc gttcgggtat ttctgttcat cggtatacca gtcgcccaaa
     1261 gcagcacggt catcgttacg tcctttaaac cagccatcat caataatgaa acgctcaacg
     1321 cccagagctg ctgcacgctc agccatctgc atgatgtaat caggattgtg attgaaataa
     1381 ataccttccc aggtattgag atgtacgggg cgtttttttt gctcaggaaa acggatgatt
     1441 tcatcacgta aaaaacaatg gtactgctga ctcattccat taaggccatc cgcagagtgg
     1501 caggcataca accacggtgt gtgcaggggt tcattcttcc tgagagcctt ctcaccaggc
     1561 atccacagag cctctgcctg tacgtaacgc ctgccgtcag tttttgcctc acatctcatg
     1621 cggtgatttc cactccaccc cagatgcaca gcccatacct ctccttgttg ttctgaaaag
     1681 cctagcgtgc cgacaatcag caccggaaaa tgctcatgag atgtccgcct tctgcgattt
     1741 tccagaacaa aagcatcatg agtgagacgt accctgtgcg gctgaaattc acgagtccag
     1801 cgcccgtgaa aggccatgac ttcttccgca cgttcagcta caggtaaagt gatggcgaaa
     1861 cgatttatct gccagtctcc ctctctgagg ttggtcagtg catgacgaac tttcagaatc
     1921 cccgatggcg tcatcaccag ctcgctgacc agacgcagtc cggctatcga atcttcacag
     1981 gtaagtggta tgttccacgt caactgtata aaaaacagga gatgcatcca gtccattcct
     2041 gtgtccttcc agtcccggag agctaaaaag ccccagggca ttttcagcca tcattgtcag
     2101 tggcgtatca atatccagac gaccattcgc aaccggtctt tccaggctgg cacagtcatc
     2161 cggtgagaaa tgtttcagtg cagagcccca ccagataatt tctgcatgtg gatgggtttt
     2221 aataattaaa tcagagcgag gactgctcag tctgcagtac tttgaaatca taagacatct
     2281 ccgctttcag gcatgtctta ctgttgatcc gaaacgtttc ggatatgtga cctggataaa
     2341 aaatcagaag gaaggagaat gaaccgtact accagggtaa aataccggtt cccagactat
     2401 ctgcagtgat tctggtgagg catcattgat tatctggtac accatgtcag aaatctgacg
     2461 accaacgaga cttcgtgttg actgaataac agcagccaca tccagctcaa taatgctgtc
     2521 ctgtggcaaa ccatcatata caaccagaga tacagcttcc ttccctgaaa gacgccctct
     2581 caactgcagc gccatagccg caccatctcc actcaggtca ttgtcagtaa taatagctgt
     2641 tggtggcttc ggtaatgaca ttaactccat gacagccaga tagcccgctc gtcgcgtggg
     2701 agaaaccatc cgcaacaatg aatcttccag tccatgctca tgcagtgcat caagccatcc
     2761 ctgacgtctt gcaataacat aagaatgtga actgttctcg ctcaacagcg caatactctt
     2821 atgtcccaaa gcaatcagct tctgggttgc ctgctaggtt ccggcatgat tatcaaagtc
     2881 aaaccatgca cagggcagac ccggcggcac ccgcccaaga gccagaaacg gaatacctgc
     2941 tttatgaaga tgagtgatac ggggatcgtc atccagagta tgtgcaatga taagagcatc
     3001 aattctgcga ctttcaacaa gccgcatata gttgtagcag tctgtatgct catcatcagc
     3061 gatcagcagt aagtcaatat catgataagc aagttccctg ctgatacaac tgaccatatc
     3121 cataaaaaca ctgctgttaa acggcacatc attttcagga taaaccagac cgacagcatc
     3181 ggttttaccg gttttgagtc ttcttgcctg tgtattaggc cggtaaccgc gtcgacgagc
     3241 ttccgcttcc acgcgctcac gggtagaagc agccacatct gaaaagcctc caagagcccg
     3301 acttacagtg gtgacagaaa taccgagtgc cgtggcaatc gccttaagtg acatattatc
     3361 tcctgcttat atctgataat gcagaaagta tgcgggtata tacatcagca ataccagata
     3421 tttatctgta gtggttcaca aaatcaggac acaaggataa ccggtttcca cttttcttca
     3481 tgtaagcaag ccggtaaacc atcgttattc gtgtgaggtc gataaacgtt gtactaacta
     3541 taatccactg gtttatatcg cgtaccgcat ggctaaaatc accataatca cctttcggaa
     3601 gccatccact tttaaggctg cgaaagactc tttccatcgg cgaattatcc aggcaattcc
     3661 ctctgcgact aatactttgt attaccccat aacggcagag taactttctg tatttattgc
     3721 ttttatactg aacaccttga tctgaatgaa acagcaggcg gccatcacgc tgtcgtgttt
     3781 ccagtgcatt acgcaaagcc ctacacacca actcagcatc agtggttaat gagagggttg
     3841 aaccgataat ccgccgtgaa tataaatcaa caacgagcgc gagataacac catttgtcct
     3901 gcaagcgaat aaaactgatg tcgccgcacc agacgcggtt tgatgcgacg gagttaaatt
     3961 gccggttcag taaatttggc aggacttttg tcttcgttta cccggtagtg atgtttaacc
     4021 ggctgtcgac ttgtcagctc gcattcccgc attagaggac gcttcacctg cgcgtaataa
     4081 acgctacggt gaataccgaa taaatgacaa agaccccgca ctggccactt tgcttttagc
     4141 cgtgtgatta gcgcgacagc ttcccgggga tttcgctcat cagcacggca gcctgcttta
     4201 gtgtttcttt ttccatctca aggcgcttta tctgcacttt aagctgctga atttcgcgtt
     4261 gttcaggggt aattgcatta ccagcgggct cagtaccctg aagtccctgc ttttacaact
     4321 gtatccatgt acgcaaatgg tctgggttgg gctcaagtgc cagcgcgact tctctgacat
     4381 cacgctggta tttaaccacc tgtccgatag cttcaagctt gaactccggg gaaaaggtac
     4441 gtttagtccg acgagttttg atcatgcatc acctcatttt cactgtttta acattaacag
     4501 gatttcgagg tgtcctgaat tacagtgcca tatcatcagt cagtggttac aggtgagatt
     4561 acgttctccc tctgcggact ctgcggacag cccgtggttt tttccttcac gtaacggccc
     4621 ccacatcacc cgacagcgtt tctggcagat tatccgcgaa tatgccgtca tggccggact
     4681 ggccgtcagg gttcatccac acatgctgcg ccatgcctgt ggttttgctc tcgctgaacg
     4741 cggaaatgac acccgactga tacaggacta tctcggacac aaaaatattc gtcacaccgt
     4801 ccgttatacc gccaccagtc cggcgcgctt tcgacaggca tggatacatc ctccctgtca
     4861 caccctgagc cccgacaaca catcaccgga acacgacgtc atgcataccg gataccacat
     4921 ccgctcatct tctgcccaca ttcttccgcc acatctttcg gtgatacatc atgaatacgg
     4981 gaatatgatg aaaaatatct gcagggaact ggataatata cagcatatac tgaaaagaat
     5041 ggatgcacgg gccactgccg gataaaagtg aacgggctca gggaggacgt ttaacgtata
     5101 cggatgcagt cagtcactcc cgcagaccaa aaagaagcca gccatcctgg ctggcaagca
     5161 tgtcatgtac ctctggcaaa taaaagcact ttatcggaca tcagtgctta cacacataat
     5221 attatctgcc gtacgtcatt acggatatta tttcccgtcg ttttcaggaa tcagttatta
     5281 tccgcgagcc atggttacct gagaacacta taaatgatac cgatatttat atacagacag
     5341 agattcagtc acacaagggt attataaaag gcatgaacgg agacaggtac gggacagcac
     5401 caccacaaaa aaaaagacct gcacacggca ggtctgcaat gcaaaagaaa tctcagaaag
     5461 tcagtaatga tgaaatcaat aaatgctgat ggtctgtaaa taacgcctca tccccctcac
     5521 gggattttcg ccctgtcatg ctaaccagca gaaaacagaa cacaacaccg gcgccttata
     5581 aataagattt tttttatatt ctgtcccgtc gccgtcaaaa aaaaagccag ttccggtgaa
     5641 ctggcaaatg tacggaacaa acagtggaca cttcgtctca gaaggggaga acttccgcgg
     5701 aagataatgt tattcagacg tcagaatgac tgcaataatc tgacgctatg tttcagaatt
     5761 ggttatctgc acatatccca aagacatgtc aggagtctga caaaaaatat caacaacgtc
     5821 accggcgctg gtatatttct ctcagaaaga ttcttaataa tataaagaat acagccccgc
     5881 atcatatctg aacggggaca aaaaaataac ttcctccgaa acaggatgaa ataactcttg
     5941 aaatcccccc cccggattga agattattta tcgggcagtg acatcttctc tgaagaaaag
     6001 ttcaatctca aaggttcagt aaaatgaaaa agctaacggt gacaatttct gcagtagcaa
     6061 cagcagtcct gatggctgta tcagcacagg cagctgaaat atacaataaa gatggtaata
     6121 aacttgatct gtacgggaaa gtggacgggc tccattattt ttcaagcaat aaggatgaaa
     6181 atggtgacaa atcctacatg cgtatgggga tcaaggggga aacccagatc aacgatgatt
     6241 tgacgggata tggtcactgg gagtaccaga tccaggcaaa cacgccggaa aatgaaaacc
     6301 atgcctggac ccgcctggct ttcgccggcc tgaaggtctc tgattatggg tctctggact
     6361 acggacgtaa ttatggtgtt gtctatgacg tcacctcctg gactgatgtg ctgccggagt
     6421 ttggtggtga tacttacagc gcggataact tcatgcaggg acggggtaac ggttttgcca
     6481 cctaccgtaa caccgacttt tttggccttg ttgatggtct taattttgcc gtgcagtatc
     6541 agggtgaaaa tggcggcgtg aacggtgaaa atacgacaaa taacgggcgt ggtgcattac
     6601 ggcagaacgg taatggtgtt ggtggctcaa ttacctacga ttatgacggt ttcagtattg
     6661 gtggtgcggt gtccagttcc cagcgtacat gggatcagaa ccatacggga ctgatgggga
     6721 ccggcaaccg tgcagaaacc tacaccggtg ggctgaagta tgatgcctat aacgtctatc
     6781 tggcagcaca gtatacccag acatacaatg ccacacttgc cggcaatctg ggctgggcaa
     6841 acaaagccca gaaccttgaa ctggtggccc agtatcagtt tgattttggt ctgcgcccgt
     6901 cccttgcctt tctgcagtcc aaggggaaaa aactgggaag catcaatggc cgcaattatg
     6961 acaaccagga tatcctgaaa tatgttgatg tgggcgcgac ctactacttc aacaaaaata
     7021 tgtccaccta tgtggattat aaaatcaacc tgctgaaaga caaccagttt acacgtgatg
     7081 ccggcatcaa taccgatgat attgttgcgg taggtctggt ctatcagttc tgacggaaag
     7141 accgacgtat attccgggat gatttatcat cccggaataa cagtggctcc ctcaggccac
     7201 tattaaaaga cacctgtctt tttatcaaca aatcttctgt tatattttta tttgttcagg
     7261 gcgttatcac gccctttttt atgtgcagag agtcatcggc ctgttaacct caagggggct
     7321 ttctgtttat tttcagccat ttcacttacg gcattatcat aatagtgtaa tggtgtcatc
     7381 ccataataat ccttaaatgc tgagatgaaa tatgacgtgc tgttgtagcc acacttttgt
     7441 gcaaccatgg agacagaatg tgtattttca agtatcatct ttcgtgctga ctccatacgc
     7501 atatccctta atatttcaga gaatgaggtt ccctcctcct tcagtctttt cttgatcaga
     7561 ctttcactgg catagaggcg tccggcaata tctttaaggt gccaggcacg ttctaaatca
     7621 cccccgataa tccccataat cttatacgag aatgtgttca tatacgtcag taagaaagaa
     7681 atcacttttg ttcgctcctg aaatacagaa agcagggaca aacagagttt ttctcttagt
     7741 gatgtcgtgt aagcgtctgc aattcttatc ccttcaaaaa gagaatagac cagcgacagc
     7801 ggaatatttt cctcgatgac atatccctgt ctgtcaatac agaacatatc aggcagtctg
     7861 ttattcacat cattcagaaa ggacaatact gttttatggt caagttcaac cgttctgacg
     7921 agagaggggc agtcagacaa tttacttctg gcagcaggct ccagaagtaa aacagtgttt
     7981 ttcctgaatg atacaggctt cttacagata attaagtcga aaggctgaca aaccagaaca
     8041 acggaacata cagcatccat caggcacctg cgacagattg aaaaagaaga atattaaata
     8101 aaggttttaa cgaataaatt tgtcacacat tttaaataaa aaatatcatt aatcaaatgt
     8161 tcataagaaa catttaagaa atatatgata tatatatatg acaaaataat atataaatat
     8221 tgttgttttt ttataaacca cattatcaat aacgatatcc actcaactta ccccgtctga
     8281 gtaaacagta atttacagta ataccatcga acggaaaatg tcactgttgc agtgtaactc
     8341 atcagatctc ataaatgtta ttgttatatt aaattagtgt tgtgtcccat gagggcacgc
     8401 aagaagcaca cagaaaagca acaaaacatt aatcagatca aaacgcaagt cattccccct
     8461 tcctgaaagg gggctttttg ttcagtgctg tcttcgccgc cgaaattttt cgtaccgttc
     8521 cgcaggataa gctcgcccgc agcagataac gatgagcaga aacagagctg tcggaaaaaa
     8581 aacacatccg cactccctga aaaaaaccga tatcatgctg atttcgccac agccttataa
     8641 ttatcctgcg tagcgactgt tcacccgaat ctggaggatt acttttcctt tatcaataat
     8701 ccggttatcg aaaacttaac tgcaatattt gaattattct gtttcgttat atcagtttct
     8761 gtggttgcca tttacttaat atttatattc tgaaaaaccc tttcagacaa atgaaatagc
     8821 tgtcgcacca taactgttac tacagcgagt ataaacattc agagaacgga cttattagct
     8881 ataaaaatca cctggcggaa aatttactaa tttatggatt gtgatattat taaatagtaa
     8941 aaccagccag ccgaggtatt tatgggaaaa catcaggaag aaaggaaaac ccccgtcatt
     9001 gtggtgaaaa aacgtcgaac tttttcgcca ccatccttat ccgaacaaac agatattatc
     9061 gtgcctgtat ctactgaaca ggcgacagaa tcaacacccg ccggaataag ttcatctgcc
     9121 gtcgaaacac atatccccga agcgccagcc cggaagaaga aaaagaaaag acaccgtttc
     9181 ccacgtcccg tccactggac ccgtgagtat actcacgagt gcgtggaaaa aataaaaacc
     9241 ctgtttcctc atcttcgtgc tgaaggcggt ggctttcttc ccctgaaaat tggcatcagc
     9301 aatgatattt cggcatttct ggcagaaaac ccggatacag aactgtccat tgacgaatgg
     9361 ttctgtgcag tgtcctgcat cacctcccgc cgcgtatagc tgcagcggat ctctgtcgcc
     9421 ggcgtccctc gttatgggct ggatggtcat cccgatgggc aggtctcaga aactgaagca
     9481 cagagtgctg gtcaaagact gacgataata gaacaacagt ggctcaggat gaaggagcgg
     9541 cagactgagc aataaacatt tcagataaca tcatgacatt gtgtatcaga gtctgcttac
     9601 agactcatgg atgccaccaa aggctcatca gatgtaaggc agaagcgacg taactgcctt
     9661 ttgaaaggaa aaaagcgacc gcaaggatgc aatggatatc tgccgcatac tgaaataaag
     9721 taacaaaaaa atccccacaa tcacgggggc ttccttcctg tactcagccc ccgttacttt
     9781 attcgtaccc cttataatgg ggtgttagcc agccagaccc ggcatgatta ctgcccccag
     9841 tcgtccatga accggggggt gatgtcaccg ggtctggtgg ggcgctggta accgctaata
     9901 gggggcaggt caggctcttt tgccgggacc gtctgtaacg tggatgccgg tacctgctcc
     9961 ccgtggttat ctggttaacc catatacaag ggagacagaa tgaccgaatc cagcgattac
    10021 gaatccgtcc aggtctttat cggcgttgat gtcggtaaag atacgcatca cgctgttgcc
    10081 attaatcgtt caggtaaacg cctgttcgat aaagcattac ccaacgacga aaacaaactc
    10141 aggtcgctaa tatctgacct gaaacaacat ggtcagatac tgctggttgt tgatcagcca
    10201 gctaccatcg gtgcgttacc tgtcgccgtt gcccgctcag aaggagtcct tgtcggatac
    10261 ctccctggac tggccatgcg ccgcatagcc gacttacacg ccggtgaagc taaaactgat
    10321 gctcgtgacg ctgccatcat tgccgaagct gcccgtaccc tgcctcacgc gctacgcacg
    10381 ctgaaactgg ctgacgagca aatcgccgaa ctctccatgc tctgcggctt cgatgatgat
    10441 cttgccgcac agacaacgca ggccagcaac cgtatccgcg gccttctgat ccagatacat
    10501 ccggcactgg agcgtgttct cggtccgaga cttgagcacc cggcgatact cgatcttctc
    10561 cagcgatatc cctcaccaga aaaactcgct tcgctgggtg agaagaagct ggcagcccag
    10621 ctctgcaaac ttgcgcctcg tctgggtaaa cgccttgcag cagacatagc tcaggcacta
    10681 gccgaacaaa ccgtcgccgt tcccggcacg aatgccgctg ccgtagtact gccacgtctg
    10741 gcactccagc tcatcacgct gcgtaagcaa agagacgagg tggcgcttga ggtagaacag
    10801 cgagttcttg ctcaccctct ttacccggtc ctgaccagta tgcccggagt cggtgtcagg
    10861 accgcagcca gactcctcac cgaggtcgcc tgccgcgcct tcgcctctgc cgcacatctc
    10921 gctgcttatg ctggccttgc gccggtaact cggcgatccg gctcgtcaat acgcggtgag
    10981 catccctcgc gacggggtaa taaagctctc aaacgggcgt tgttcctgtc ggccttcgcc
    11041 gcgctcaggg atccgctctc cagggcttac tacacccgca aaatgagtca gggaaaacga
    11101 cacaatcagg cgcttatcgc cctggcgaga cgacgctgcg acgttctgtt cgccatgatg
    11161 cgcgacggga ctttttatac cccgcaggcg tcataacatg cttgacaact taataggggc
    11221 acccctcatg cctgagcatc gcatgcaggc gacgatagcc ataacactgg taattactgt
    11281 taaagatatc tgtcagcatc gtacgtatcg tggcgtattt atcaccggct ttcagtgcag
    11341 cccggtgata aaaataactg ctacgggcaa ggcccagaac atgcagtaat tctgtcaggg
    11401 gatatgtttg tctcagggca tcagcgatct ttgttttttc tctgttgttc tggtgactga
    11461 tactgatgtc tgggtctttt tttatgattt cctccgcctt tttcagaata tccagctcca
    11521 tctgccggcg gcgtatttcc tgattcagtg gggcgacttc ctcccgcagc gcatcgcgtt
    11581 ctgcctctag ggaaggttcg ttatgtttac gcatagtctg gtaagcactg ttgccgataa
    11641 tttcatcttt ccatttatac aatacctcac gactgacacc aatacgcctg gcgatttcac
    11701 tggctgatac ttgtcgtgta cagagtgcca tgacagcctg acgtttgacc tctggttcaa
    11761 agggcacaac cgggttggcc ttactgataa atatacgtcg tctgtcggga taaagttcat
    11821 taacccagcg agcgagcaca tcagtgcagg gataacccag ggctctgctg gtaaacgcca
    11881 ggcagcagcc gtggttaaga taatgttcaa cggcaacctg tttttgctca tcggagtaac
    11941 ggtgcttatg acgaatggat ccttttacgc caccacctgc ttcccatgag cggatccaac
    12001 gacgcagatt ccttttcgaa gggtagccca gttcgcgtac gacgggagcc agcttcttac
    12061 catatttgaa ataaagctca atggcgcgga ttttttcttc atcagtaaac atgttttatc
    12121 tcctgagagt ccaggaaatc gtccgcatcc ccatttacca tcttatatca ctggcgctga
    12181 cagtatatct gcaatcaggg aactggccgg aaaagtgaat atcccgacag gtctgaaagc
    12241 cctgaacgtc agagaagagg atatccctgt tctggccgct aatgccctga aggatgcatg
    12301 tggtctgaca aacccgattc aggctacaca ggaggaaatt gaggccattt tccgctcagc
    12361 aatgtaagca caggaccttc acagacggtg aagaacgatt aaaatattcc agcacatttc
    12421 cggtgagtgg cgatgcgtca acagcaatcg ggaatgacca cagagacacg gaaatgtgcc
    12481 gtttccataa aatgcccgcc gttcttcaga ataattcaaa ctcagcattc acttttatgc
    12541 tgcccgtgct cctgaagcgc cagcatccgg tgaggcgtga cgccaaaaca ttccctgaac
    12601 acgcagataa aataagatgt gcttacataa ccacactgcg cggccaccct gttgaccggc
    12661 agacgggcct gtaacagacg gcgtgcatag cccattctca catccagaag cactccggtg
    12721 aacgaacatc cttcttcctt cagtttcttt ttcagctgac tttcactcat atacaaacga
    12781 ctgctgacat cccgcagctt ccactggcgt gaaaggtcag tacagatgat atgcctcact
    12841 ctcccggcca cagaaagcat tccgcccagc aacaaaggaa gaaagccttt ttttgatgca
    12901 aagacagcaa tgcaggaaaa aaacatctgc tcggaaaatc cgggatgaac aaatcctccc
    12961 gaattcagat gatgcataac gtcacgtaac aacgcaggat gagcaaaggc cactctgatg
    13021 aaaccagaag aaaccgtgtt gtcggcctcc cggttgccgc cacaactctc cagatatctg
    13081 cggatgatat tttcatccag ccgcacctca gtgatatgcg cagcatgagg ctgaaaatcc
    13141 tcacgatcag tatcatcaat cagaagcaca tctcctgcag aaagggacag cattttcccg
    13201 gcaaactgca gggtgaatgc cctgtcagcc agtacaacag aacagacaaa aatcatgttc
    13261 actccggaac agaacaggga caaaagtatc ttaataacta taacataccg ggaagaaagc
    13321 atgaccgacc agagcagagg tgaatatgat tactgataaa aaagctgaag agctcgaaca
    13381 gcggggatta tggcgcagag ccgctgcacg ctggcaggaa ttgctgctga actacctgca
    13441 ggaagatgat cggatctgta tccagagacg gatacagcac tgcctgaaac gtgcaaaaat
    13501 gaaatcacag agagcaacca gcctggaaga gttctccttc agattattac gcagttctgc
    13561 aatacagacg gaaaaagaaa tgggcatata cagggaccgc aaaacagcac atgatcattt
    13621 atatcgccag gatggtaaaa aatctccgca ctgagttcca gccctcccgg tgcaaccgta
    13681 gaacggcacc gggcttacta ggaattgtct cattattatt gccgtaacaa aaaaacagtt
    13741 ttctctgatg aatgaattat taatataact ccggaaaagc acctttcttt ttaacctaca
    13801 gaaggtgttg ttatgaaaaa atattcttca gccgtccttg cactcattct caccagcacc
    13861 tgtatgacag cctccgctgc cacacaagaa atctcaaaaa aagcctatgt ctgtgatgga
    13921 aacaagacaa tggaggttgt ctatatcaac accactgccg gaaactccta tgcagtgata
    13981 aaccagatgg atgagatgat acccatgcag cttatgaaaa tagcctccgg agcgaattac
    14041 gaagccatca acaaaaacta ctcatataaa ctgtacacaa aagggaaaaa agccgatctg
    14101 gtagaaggga atgacagacc tgtattaagc aactgctcat cagcagaatg acagccatca
    14161 tgctgaccgt gatatagtga tattctgttt tctgtatcac attttttatc tttcggtttg
    14221 catcctgttt ctgcatgttt taaaactttc atttaaaatt ataattaaca gactatccat
    14281 cgaatcggtt tttactttat gccctcacta tctcctctga gggcattttt ctataaaaca
    14341 cctcgcatta accatgcctg ttattttgtt ttataaaaaa caaaataaca aaaaataact
    14401 gaatgtactc tcacgatgca ttttgcttcg taaccgctgt ttttatttat tgtgttcttt
    14461 tctgtcggca ctgctcatac cattcccgcc ggatacgctc acctgtctcc gcgcgtatcc
    14521 gggcatgata ttcctgctcc ctgcgtgcat gtaattcacg ctgcaccgcc agtgcattca
    14581 caacgcctgc cacgcaacag ataacaatga gcagaaacag aactgtcgaa aaaaaacaca
    14641 tatccggtac tccctgaaaa cacggttcca gtaccggtat catgctgttt tcgtcacggc
    14701 caggcaatca tccggtgaag cgacggtttg ccagaatctg gtgaagtaca ctgttcagaa
    14761 caagcgtatt ccgggcccgc gtggcagcca catagagcag gttgatttca tcccgccgtt
    14821 tctcttcatt tttcggttcg ttatccatac ttgtaagtat cccgtataat cgaaccattc
    14881 acatttagag atcttccggc atacttattc tgccaacgaa ggagagcgtt atgcgtaaag
    14941 cccgtttcac tgagcatcag atcatcgctg tgattaagtc ggttgaagcc ggacgaactg
    15001 tcaaggatgt ttgccgcgag gccggtatct ctgaagcgac ctactacaat tggaaatcta
    15061 aataaggtgg tatggaagcc tctgatatta aaaagataaa ggatcttgag gacgagaaca
    15121 ggcgtctcaa acagatgttt gctgacatca gccttgagaa ccgggcattg aaagacgtta
    15181 tcgaaaaaag ttttgaaacc agcctttaag cgtgagctgg tcactcatct gataacgacg
    15241 ttcgggctta gtatccgtca ggcctgtcgg agcctgaacc tgagtagaac ggtttatcat
    15301 tatcgtccga atactacacg tgacgagccg gttatttctg cactgcaggc tgccgctgaa
    15361 cggtatccac gatatggttt tccaaagttg tttcagattc tgcgacggca gggatacccg
    15421 tggaatcaca aaagggtcca ccgtatttac tgtctgctga agctgaattt tcgtcgtaaa
    15481 ggcaagcagc gtctgccggt acgtaatcca tcgccgcttg ctacacctga agcgttaaac
    15541 cagcgttggt ctgttgactt catgcatgac gccctgattt gtggtcgtcg ttttcgtacg
    15601 ttcaacgtcg ttgatgactt taatcgcgag gcgttgtcta tcgaaataga ccttaattta
    15661 ccagctcagc gagtggttcg tgtcctcgac aggatcgcag caaaccgggg atatcccgta
    15721 atgcttcgca tggataatgg tccggaattt atctcgctgg cactggctga atgggtagag
    15781 aaacatacag tacaactgga atttatccaa ccgggtaaac cgacacagaa cgcattcatt
    15841 gaacgcttta acagaacata tcgcacagaa atactcgatt tttatctgtt cagaacgctg
    15901 aatgaagtgc gtgaaatcac ggaacaatgg ctgtcagaat ataactgtga acgccctcat
    15961 gaatcactga acaacctgac accgtaggaa tatcgtcaac aacattacca gcccgggatc
    16021 tcaaaaagtg tatggaacta aagcgggtct atttacagca acagcgatac tggatcggtt
    16081 gctacatcac tcaaccacgc tgaatatcaa aggagagcgt taccggttaa aagagaaacg
    16141 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtgcaaag
    16201 cggacagcat cagtaacgaa agtatttagc gggcatgaaa atggcaaaga acgatcaaac
    16261 atcgtggcgt tgacaacggg ttcctgagtc cagtgaagcg ccggttactg gaaaatgttg
    16321 tgtacgtcat aacagaaacg gagagaaaaa cggcgatgca aatcaggcgg agaagggtgt
    16381 atcagcggtt actgaaggtt gacccgctga agtgcatcct gtgcggaggt cagatgcggt
    16441 ttacggggat gaagcggggc taccgtctgg cagagctggt catgatgcat gagccactgg
    16501 cacgacagcg ggtatacagc tgagagccgc agaggggaag ttgcgtccat tttttgagga
    16561 atggagcaaa aaatcatcac agatataaaa aatcaatcaa tgaaagccat ttaattgccg
    16621 gtgcattgtg gatgcagtcc tcatggtacg taaccagagt tgaataaaca tccatttttt
    16681 tgtgtttttt taacctcccc gggtttttaa ttcctgtgga ttaaaagtgt tagattattt
    16741 ggttaagagt tctctcgtcc tatttattca ggcgttatca cgaatatgca gatcgctaag
    16801 tcagttgtaa gaaaaccaga cagtgcacat cattatacct aggtcttctc tggaaaaaat
    16861 acttaggaat aatttatgta aaacaggttg tcgtcttcat gatttaaacg cagtacatat
    16921 atatactcta taataaatac agggagtatc acgagacgtc tggataagag gaataaccag
    16981 aataaattgg taaggaataa agacacttat ttctattgag aaaaaacatt tatcctgatg
    17041 tgattagtat caatgaaata atgtgcttgt atggttgata aaaaaatgca tcactaaaga
    17101 aaaaaacagt atgaataaga atatacgaat tttacagttt ctggtcagta tactttattc
    17161 tgtgcagtct catttttctg gtgcgcaaac aatacaactg aatggtaatg gtatacctga
    17221 aagtataacc aggagtatta caggtgttga tggaaacgcg gcgcttaata tcagcgtgcc
    17281 atataaaaca agttatactc aaaatatact atctgttgaa agcagtatta acatcaaagg
    17341 tggaacaagc aatacatcaa tcggtggtgc aggtgtctac ggtgaaaact ttacgctaaa
    17401 taataatggt agtgtttggg gaggagatgg atataatggt ggagttgctg ttagtggcaa
    17461 caaaatatct ataaacaatt acagaaatgt atatgggggt aatggtcttg gtggctcagg
    17521 aagtagcgga ggtgcagggt taagcgggga tgatattata gttgataatt acagaagtat
    17581 atacggaggt gatgatctag gtgggacagg tggttccggt gtaaccggga acaatattac
    17641 agtgcataat tccggaggaa tatggggtgg taatggcgta aacggtggtg atggtattaa
    17701 tggtagtaat cttttcatta ctaacgacaa catgatatct ggaggatatg gaataaaaca
    17761 agggggagat gctatttctg gaaatcaaat cactttgaat aataacggta ttgttcaggg
    17821 gggatatggc cccgacggta gttgctctgt ttatggagaa gatatccata ttaataatca
    17881 tggtaatatt tcaggctcat ataatagcca aaaagatgct tataatacat caataatttt
    17941 ttctggtggg tataattcat tagatattta ttcagattct gtgattaatg gtgatattaa
    18001 actagctagt atacctgtta atggtacaaa tgaattaatt attaaaaaca tcaataacgc
    18061 aacagcaatt aatggtgggc taatgattgg gaatggctcg tctgtttatc tgtcaggcaa
    18121 gaacatcatt tttaacggaa atataagtat tgatgaagac gcatctatga acctgtctgc
    18181 aggaaatgct aatgttcacg caaatactat tacattaaaa agtgattcat ggcttaatat
    18241 agacacatca attaagaact ggactcagga ctattacaca ttattgtcgt cagacacagg
    18301 tatctcgatc gctgataata gtcacattgt acaatacaat gtattactga cagaaggtgc
    18361 tgaaagttat gtttatacgt ctttaaatga cgacgataac aaactgatat ccatgctgag
    18421 atggaataat acaaaaggga tgggatatgg aacctttaat atagaaaaag atgccactct
    18481 gaacatagga gtttctcttt ccgataatct ttcaccttta ttatatgatg gctgggacgg
    18541 caaaagtctg acaaaatcag gtaatggtac tcttatactt tctgcaacaa acaactatac
    18601 aggaaataca gaggttaaat ctggcgtatt aattcttgct gcacctgatg ctcttggtcg
    18661 gactgagtat ttatatttat cccgtggcgc agaactggat atgaatgggt atcctcagac
    18721 aataagcaaa ctactgacgg ctgcaggctc tgtgctgaac attcatggcg gaagcctgat
    18781 actgaataat ggaggagaat ctgcaggtac tattgcaggg gatggttctc tgaacataaa
    18841 tgggggaatg cttgatataa cgggtaataa tcgttatttt tccggtgttt ttaccgtgaa
    18901 taagggggct cagttggctg tatccacggc tgataatctg gggacagcct ttgttgataa
    18961 ctatggcaca ttaactctga acagtacatc agcatggcag cttaccaaca atatcagtgg
    19021 ttatggtaat gttcgcaaga cgggagcagg tgcactgaac attagcgata acgcaaaatg
    19081 gaccgggatg acagatatta ttcaggggac attgattctg ggtaacgcag attcaccggt
    19141 gatgctcggc agtaaccagg tcattgttga agagcagggc aaactctccg ggtttggggg
    19201 cgttgcagga aatctgagca atagtggtat agtcgatctc actacatata tgccgggtaa
    19261 tatactgact gtcggaggga attacactgg cagaaatgga cttattctcc tccagacaga
    19321 aacaggtggt gacaattcga aaacagatcg tctggtgatt aaaggtaatg ccagtggccg
    19381 tacccgtgtc gctgttactc aggccggtgg tactggtgca gagacactta atgggattga
    19441 agtgattcac gtcagtggca atgctgataa tgctgaattc attcagacgg aacgtattac
    19501 agccggagct tatgattaca tactgaaacg tggtcagggg attaacagca ctaactggta
    19561 tctgattagc agaaaagaca ttcctgtacc acaacctgaa gctgtaccgg aaaaccatga
    19621 taataatttg cgtcctgagg ctggtagcta tgttgccagt attgctgctg caaataatct
    19681 gtttgtaacg aatctgtatg aacgacaggg acaggagttg tatatcagcc acatgccagg
    19741 agaagaaaat gaagcaggta tctggatgta taataaagga aaacataatc gctggcgtga
    19801 caacagtagt cagctgagaa cccgggggaa tagctacgtt gtgttaatag ggggagatat
    19861 agctcagtgg agcctgaatg gtaccgatcg ctggcataca ggtatgatgg ctggctatgg
    19921 tcataatcat aacagtacga atgccctgag caccggatac cattcggaag gaagaatgaa
    19981 tggatacact gcgggtcttt atgcaacatg gtatgccaat gatgaaacac acaatggttc
    20041 ttatcttgat agctggctgc aatacagctg gtttgataat catataaatg gagaacggct
    20101 gcctgccgag tcatggaagt caaaagggtt tacggtatct ctggaagcag gatattcatg
    20161 gaaggctgga gagtttaccg acaattacaa gggaagtcat gaatggtatg ttcagccgca
    20221 gcttcaggtt gtccggatga atgtaaaatc agacaaatat catgaaagta acggaaccag
    20281 tattgaaaat accggtaacg gaaatattct cactcgcctg ggagcaagaa catggcttac
    20341 cagcaaaaac ggtaaaaata cgcggtatgc ggttccgttc agaccatttg tggaggcaca
    20401 ctggttgcac aatagtcgtg ttttcggcac cagtatgaat ggtgtaagta tataccagga
    20461 tggggcgcgt gatatcggag aaataaatgg tggtgttgcg ggaatgataa caccagaagt
    20521 agcattccgg gctgatgcag gcattcaact tggagtacat ggataccata atacatctgc
    20581 catgttgagt gtggaatatc gtttctgatg tgtctttaca ttataaatat ccttcacaaa
    20641 atattgttga taagacatgg ttaggtggtg agctgcctcg gaaaatatga cgacagtaat
    20701 gatttctaag agatttgaaa tgcgttgata acaaaatctg aaatacagga gtctgtttag
    20761 gattgtgtgt aaatgccttt tctcagaagt aaccgtccag gcggtcactg aatccgataa
    20821 taaagcggtt tattgccata ccccagtcct tcaacggcat cgtccatttc ggggacgatg
    20881 cctgtattgc cagacacacc acttttttca ccggctcgtc tgtcggaaac acttacgttt
    20941 tttgatcgca tggcggatca cgctttttag cgacccgatg gcattcgtca gcgacagcga
    21001 cgactcttac tgctggaacg aagtggataa gcgcttcagt gtcgacgaca cgccgaacga
    21061 gccgaaccgc ttcggctggg tgggggaaat cgatcaatat ggttattttt cagaaaataa
    21121 tgagaacatc tgtgctttat ataaccagat aaaaccacgc agccagcata aatattactg
    21181 aaatgataat attttattta tcacttcggt tatatt
//
LOCUS       DAHBHC010000085        19873 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHBHC010000085 DAHBHC010000000
VERSION     DAHBHC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.085"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..647)
                     /locus_tag="M2A35_003912"
     CDS             complement(<1..647)
                     /locus_tag="M2A35_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200144.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRI"
     gene            complement(1015..2355)
                     /gene="fadL"
                     /locus_tag="M2A35_003913"
     CDS             complement(1015..2355)
                     /gene="fadL"
                     /locus_tag="M2A35_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCB7200145.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKTDNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            2727..3011
                     /locus_tag="M2A35_003914"
     CDS             2727..3011
                     /locus_tag="M2A35_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCB7200146.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            3191..4501
                     /gene="fadI"
                     /locus_tag="M2A35_003915"
     CDS             3191..4501
                     /gene="fadI"
                     /locus_tag="M2A35_003915"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCB7200147.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            4501..6645
                     /gene="fadJ"
                     /locus_tag="M2A35_003916"
     CDS             4501..6645
                     /gene="fadJ"
                     /locus_tag="M2A35_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCB7200148.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6848..7333
                     /gene="sixA"
                     /locus_tag="M2A35_003917"
     CDS             6848..7333
                     /gene="sixA"
                     /locus_tag="M2A35_003917"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCB7200149.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            8017..8580
                     /locus_tag="M2A35_003918"
     CDS             8017..8580
                     /locus_tag="M2A35_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200150.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            8662..11304
                     /gene="yfcU"
                     /locus_tag="M2A35_003919"
     CDS             8662..11304
                     /gene="yfcU"
                     /locus_tag="M2A35_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCB7200151.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            11324..12076
                     /locus_tag="M2A35_003920"
     CDS             11324..12076
                     /locus_tag="M2A35_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCB7200152.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            12051..12599
                     /locus_tag="M2A35_003921"
     CDS             12051..12599
                     /locus_tag="M2A35_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200153.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            12596..13066
                     /locus_tag="M2A35_003922"
     CDS             12596..13066
                     /locus_tag="M2A35_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200154.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            13063..13587
                     /locus_tag="M2A35_003923"
     CDS             13063..13587
                     /locus_tag="M2A35_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200155.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            13574..14446
                     /locus_tag="M2A35_003924"
     CDS             13574..14446
                     /locus_tag="M2A35_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCB7200156.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(14568..15119)
                     /gene="smrB"
                     /locus_tag="M2A35_003925"
     CDS             complement(14568..15119)
                     /gene="smrB"
                     /locus_tag="M2A35_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCB7200157.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            15285..16217
                     /gene="prmB"
                     /locus_tag="M2A35_003926"
     CDS             15285..16217
                     /gene="prmB"
                     /locus_tag="M2A35_003926"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCB7200158.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            16252..17337
                     /gene="aroC"
                     /locus_tag="M2A35_003927"
     CDS             16252..17337
                     /gene="aroC"
                     /locus_tag="M2A35_003927"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCB7200159.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            17341..18165
                     /gene="mepA"
                     /locus_tag="M2A35_003928"
     CDS             17341..18165
                     /gene="mepA"
                     /locus_tag="M2A35_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCB7200160.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            18165..18974
                     /locus_tag="M2A35_003929"
     CDS             18165..18974
                     /locus_tag="M2A35_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCB7200161.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18974..19522
                     /gene="epmC"
                     /locus_tag="M2A35_003930"
     CDS             18974..19522
                     /gene="epmC"
                     /locus_tag="M2A35_003930"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCB7200162.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            19556..19834
                     /locus_tag="M2A35_003931"
     CDS             19556..19834
                     /locus_tag="M2A35_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCB7200163.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         4946 a         4686 c         5136 g         5105 t
ORIGIN      
        1 ttaatacgaa ttcctctttg aatgtggtag tcgtcctgat cgtagtaacc gagagataag
       61 taatcatttc cgtttttatt tttaattaca atagcgagag agtcgccttt tttatcttta
      121 ccgtaccagg taaataagtc cgttggttcg ctattcccgc tctcatcaaa ttccgttacc
      181 gtatgtctga gtttgtgtga tatcttatcg aagataaaac caggatctcc atcatatatt
      241 tcttttaatg cgctgaagat catttccttg ttattgatat catgagtctt ctcaactatg
      301 ttgtcattaa tgatagtacg gacgagttct ttgtctttat agttcgcagg taattctatt
      361 ggttcttggt tgtcgatacc aatagtaata tggtctgtat cattggagcc aatatcaata
      421 taagtaactg aggaattatt aatattaaat ctataaggat cgtcgtttac tcgtatggcg
      481 ttttttataa gctcaagtag atgtttaata caggtgctta ttccttcctt accattaaaa
      541 aggtttttga gctcttgtac taatccatta acttcatcat tgttattttc attaatggat
      601 tctgggtaag ttgcgctggt attgttgatt gaaatagatg gtagcatgat tttattccat
      661 tttgattgat ttgttacaaa tatatattac ctaatgaaat agactatcaa aaaacgctca
      721 tttatttaga acctatcttt aatttgagtg tatgagcgca caatagccag gggggggggg
      781 agtatcctgg aaattcagaa aagtaaactc tttgagaagg acgagcagaa cgcttgaaaa
      841 tatgcatttc ccttcaggtt tgtctgtgaa taccacttac tgatttgaaa gggcaagctg
      901 ccaacaggct gtttgcaaaa ctcaggaggt aagcaatgag gacactactt tcggtgaagt
      961 gggggagtta aagtcacctg ctatgcaggt gactttatcc aggcgaacgc gttatcagaa
     1021 cgcgtagtta aagttagtac cgaacagcca ggctttacct tcagactcga actggtatgg
     1081 gccttcgtta attttcacgc tctgaccgtg catataagaa acaccaacgt cgactgaagc
     1141 atctttatta aatgcgtaag tcgtacctgc actcagccag aaacggtcct ggtccggaat
     1201 ggagatagaa cgattctgag ccggaactgg gctgtcatca aaggcgatac cggtacggaa
     1261 ggtccagtta tcatcgtagt aataagtggt acccaacgcg atgcggtaag catctttaaa
     1321 gccttcatgt ttctggaaca gcgtgtcgcc actggttgag gtcgctttca gctgctggaa
     1381 ctgactccag ctggtgtaag ccaggctata gtgaatcgcc cactgtggat caacacggtt
     1441 ataacctgac acttcccaca tttcaggcag gttcagcgtc agataacccg attgcgttgc
     1501 gccacctgtc gcggtaggaa ttggcaaacc gtagttatta aacgcacgat taagatcgct
     1561 gctgtagtta cctttgaagt caattttcac ttcagaacgg taggtcagtg catagcggtt
     1621 atttttatcc agttcataca ggattccggc gttccagcca aagccccact ggttaccgtt
     1681 cagatgagcg attttggtat tactgtcgat accgttggcg gtagctgcca atgcttgccc
     1741 ttgaggagtt tgcccggcag gagattgcat aatctgacca gcaaccaact gccccagatc
     1801 gcctgcgaaa cgttcaattt tcgcgcgagc gtagacggcg ttgaaaccaa gaccaaagct
     1861 ccatgcatta tttaagcgat acgcaccgct taagttcagg ttcatggttt caaggtcagt
     1921 tgtacccccg acagagccgc ctgcataagt atcattaaac tctgttgcca gaccatagtt
     1981 agaggtaata gaagcgcccc aaccaaattg gtcgttaatc ggtgcaacaa agtgcatgtt
     2041 cggaacccat gccgtaggcg cgatgttatc ggttttcagg ctacgaccag acggagacgt
     2101 tccgctgata ttcacatccg ggtcaatata aaccgcaccc gcagaaaatg tcgggcggtc
     2161 aaacatggta atcaatgcgg ggttacggct aacgttacct gcatcatcgg caattgcgcc
     2221 ttcccctgaa taagcccggc ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc
     2281 cgaccaggcc tgggtggaga taagtgccac tgcgactgcg agagcagact ttgtaaacag
     2341 ggttttctgg ctcatgacca taacctcaat gatttatttt tatacaaact atgttacgtg
     2401 ctgtaacagg agcgcgaagt gtagggtctg aggtacatct tagaaatcag accagtggcg
     2461 agagtatagg tcggaccagc tggaatgttg caagtatgtt tccatctttt ttcaaatatg
     2521 atctaagaaa cgcgatccag ctggcaaaat ttggaggttg cgcactcgcg gattcgggtg
     2581 aattttgttt tagatcattt ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt
     2641 aacaactgga gcagcacttt ccggaagcgt aaaatatcca caacgtttat ctggcacctt
     2701 gggtgcacat acagaggaaa tcaacgatga gtaaatgtag tgctgatgaa accccggttt
     2761 gctgctgtat ggatgttggc accattatgg acaactccga ctgcaccgcg tcttacagcc
     2821 gcgtgtttgc aagccgcgca gaagcagaac aaacgctggc ggcgctgact gaaaaagccc
     2881 gtagcgtgga atccgaaccg tgcaaaatca ccccgacttt cactgaagag tctgacggtg
     2941 tgcgcctgga tatcgatttc acttttgcct gcgaagctga aatgctgatc ttccagctgg
     3001 gtctgcgtta attcctctcg ggtacgttcg cccctgtcag ttgctggcag gggcgttttc
     3061 ttttctttct cctgtcttca ctgtgtcata gctcccactt tcttttttct ccgcttggct
     3121 aaatgtaaaa aaatggttaa gactatgatc aggtcagacc actttattta tttttttaca
     3181 ggggagtgtt atgggtcagg ttttaccgct ggttacccgc cagggcgatc gtatcgccat
     3241 tgttagcggt ttacgtacgc cttttgcccg ccaggcgacg gcttttcatg gcattcccgc
     3301 ggttgattta gggaagatgg tggtaggcga actgctggca cgcagcgaga tccccgctga
     3361 agtgattgaa cagctggtct ttggtcaggt cgtacaaatg cctgaagccc ccaacattgc
     3421 gcgtgaaatt gttctcggta ccggaatgaa tgtgcatacc gatgcttaca gcgtcagccg
     3481 cgcttgcgct accagtttcc aggcggttgc aaacgtcgca gaaagcctga tggcgggaac
     3541 tatccgagcg gggattgccg gtggggcaga ttcctcttcc gtattgccaa ttggcgtcag
     3601 taaaaaactg gcgcgcgtgc tggttgatgt caacaaagct cgaaccatga gccagcgact
     3661 gaaactcttc tctcgcctgc gtttgcgcga cttaatgccc gtaccgcctg cggtagcaga
     3721 atattctact ggcttgcgga tgggcgacac cgccgaacaa atggcgaaaa cctacggcat
     3781 cacccgagaa cagcaagatg cattagcgca ccgttcgcat cagcgtgccg ctcaggcgtg
     3841 gtcagacggt aaactcaaag aagaggtgat gactgccttt atccctcctt ataaacaacc
     3901 gcttgtcgaa gacaacaata ttcgcggtaa ttcctcgctt gccgattacg caaagctgcg
     3961 cccggcgttt gatcgcaaac acggaacggt aacggcggcg aacagtacgc cgctgaccga
     4021 tggcgcagcg gcggtgatcc tgatgactga atcccgggcg aaagaattag ggctggtgcc
     4081 gctggggtat ctgcgcagct acgcatttac tgcgattgat gtctggcagg acatgttgct
     4141 cggtccggcc tggtcaacac cgctggcgct ggaacgtgcc ggtttgacaa tgagcgatct
     4201 gacattgatc gatatgcacg aagcctttgc agctcagaca ctggcgaata ttcagttgct
     4261 gggtagtgaa cgttttgctc gtgaagtact ggggcgtgca catgccactg gcgaagtgga
     4321 cgatagcaaa tttaacgtgc ttggcggttc gattgcttac gggcatccct tcgcggcgac
     4381 cggcgcgcgg atgattaccc agacattgca tgaacttcgc cgtcgcggcg gtggatttgg
     4441 tttagttacc gcctgtgctg ccggtgggct tggcgcggca atggttctgg aggcggaata
     4501 atggaaatgg catcagtgtt tactcttaat gttcgtctgg acaacattgc tatcatcacc
     4561 atcgacgtac cgggtgagaa aatgaatacc ctgaaggcgg agtttgcctc gcaggtgcgc
     4621 gccattatta agcaactccg tgaaaacaaa gagttgcgag gcgtggtgtt tgtctccgct
     4681 aaaccggaca acttcattgc tggcgcagac atcaacatga tcggcaactg caaaacggcg
     4741 caagaagcgg aagcactggc gcggcagggc caacagttga tggcggagat tcatgctttg
     4801 cccattccgg ttatcgcggc tattcatggc gcttgcctgg gtggtgggct ggagttggcg
     4861 ctggcgtgcc acggtcgcgt ttgtactgac gatcctaaaa cggtgctcgg tttgcctgaa
     4921 gtacaacttg gattgttacc cggttcaggc ggcacccagc gtttaccgcg tctgataggc
     4981 gtcagcacag cattagagat gatcctcacc ggaaaacaac ttcgggcgaa acaggcatta
     5041 aagctggggc tggtggatga cgttgttccg cactccattt tgctggaagt cgctgttgag
     5101 ctggcgaaga aggatcgccc atcttcccgc cctctacctg tacgcgagcg tattctggcg
     5161 gggccgttag gtcgtgcgct gctgttcaaa atggtcggca agaaaacaga acacaaaact
     5221 caaggcaatt atccggcgac agaacgcatc ctggaggttg ttgaaacggg attagcgcag
     5281 ggcaccagca gcggttatga cgccgaagct cgggcgtttg gcgaactggc gatgacgcca
     5341 caatcgcagg cgctgcgtag tatctttttt gccagtacgg acgtgaagaa agatcccggt
     5401 agtgatgcac cgcctgcgcc attaaacagc gtgggaattt taggtggtgg cttgatgggc
     5461 ggcggtattg cttatgtcac tgcttgtaaa gcggggcttc cggtcagaat taaagatatc
     5521 aacccgcagg gcataaatca tgcgctgaag tacagttggg atcagctgga gggcaaagtt
     5581 cgccgtcgtc atctcaaagc cagcgaacgt gacaaacagc tggcattaat ctccggaacg
     5641 acggactatc gcggctttgc ccatcgcgat ctgattattg aagcggtgtt tgaaaatctc
     5701 gaattgaaac aacagatggt ggcggaagtt gagcaaaata gcgccgctca taccatcttt
     5761 gcttcgaata cgtcatcttt accgattggt gatatcgccg ctcacgccac gcgacctgag
     5821 caagttatcg gcctgcattt cttcagtccg gtggaaaaaa tgccgctggt ggagattatt
     5881 cctcatgcgg gtacatcagc gcaaaccatc gccactacgg taaaactggc gaaaaaacag
     5941 ggcaaaacgc ccattgtcgt gcgtgacaaa gccgggtttt acgtcaatcg catcttagcg
     6001 ccttacatta atgaagctat ccgcatgctg accgaaggtg aacgggtaga gcacattgat
     6061 gccgcgctag tgaaatttgg ttttccggta ggtccaatcc aacttttgga tgaggtagga
     6121 atcgacaccg ggactaaaat tattcctgta ctggaagccg cttatggaga acgttttagc
     6181 gcgcctgcaa atgttgtttc ttcaattttg aacgacgatc gcaaaggcag aaaaaatggt
     6241 cggggtttct atctttatgg tcagaaaggg cgtaaaagca aaaaacaggt cgatcccgcc
     6301 atttacccgc tcattggcgc acaagggcag gggcgactct ccgcaccgca ggttgctgaa
     6361 cggtgtgtga tgttgatgct gaatgaagca gtacgttgtg ttgatgagca ggttatccgt
     6421 agcgtgcgtg acggggatat tggtgcggta tttggcattg gttttccgcc atttctcggt
     6481 ggaccgttcc gctatatcga ttctctcggc gcgggcgaag tggttgcaat aatgcaacga
     6541 cttgccacgc agtatggttc ccgttttacc ccttgcgagc gtttggtcga gatgggcgcg
     6601 cgtggggaaa gtttttggaa aacaactgca actgacctgc aataagaagg tcaaagctat
     6661 atgaatccgc gctgaatggc ggagtgttgg tcaaaatgta aacgcatatt gactatactt
     6721 acgccattga ggtagaaaac agcgtttcat tcggtgaatg gataaggcac aatgccggcc
     6781 accgttttct ttctctggtt tcagatgaaa gaaaacgggc gaatctggtt aacaaaagcg
     6841 gtgcaatatg caagttttta tcatgcgtca cggcgacgca gccctcgatg ccgccagtga
     6901 ttccgttcgt cctctgacca ctaatggttg tgacgaatct cgcctgatgg cgaactggct
     6961 gaaaggtcaa aaagtggaaa tcgaacgcgt tctggtgagc ccgttcctgc gagccgagca
     7021 aacactggaa gaagtagggg attgtctgaa cctgccttcc agtgcagaag ttctgccgga
     7081 gttaacgccc tgcggcgatg ttggtctggt cagtgcctat ttgcaggcgt tgaccaatga
     7141 aggtgttgct tcggtactgg tgatctctca cttaccgtta gtcgggtatc tggttgccga
     7201 gttatgtccg ggcgaaacgc cgcctatgtt taccacctcg gccattgcca gcgtaacgct
     7261 tgatgaaagc ggaaacggta cattcaactg gcagatgagc ccgtgtaatc tgaagatggc
     7321 aaaagctatc tgatgtgttc caaattatgc ccaatgaggt agattcattg ggcataattg
     7381 ctcatcaggc agcggatatc tgtcaacctc actactatcg aagactctcc ttatctataa
     7441 ataaaacact ttctacattc attcggaaat tattcttctg cgcgtatctt gattttgccc
     7501 aaagttacat gttgaaatga ataatgtctg tttgacactc attgtcattt attaatacag
     7561 ataatagaga aaatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc
     7621 tggctatagt gtttatatat tatattgtca aaagtggata aatgccaatc gcattatatg
     7681 ttcattgttt gttttttaag ctgttgatat tcattattga tatactttct attttttcta
     7741 aatacttatt ccgtattttt taaggatgtc cttaagataa cctttcttct gtcaatatat
     7801 taattaaatc tacatttact ctttagtaaa attaaattgc atcagggggg tgaaaacatc
     7861 actatgcggc cccacaccaa agaatacaat tttctacgac ctgttctttt tggatcgcat
     7921 gccgtattca atggctgggt ataaacattg ggtattgact gaatcaatgt attgtaatgt
     7981 ttatattttt aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct
     8041 attgctgcgg caatggtgat gggcgttttc acctctacgg caacaatcgc tgcgggtaac
     8101 aacggtactg cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac
     8161 gatcacaaac tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc
     8221 accaccacgc cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tactcaggaa
     8281 acaatgacca ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc
     8341 attttcaata ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct
     8401 ctgggtacct cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct
     8461 accaataaag gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct
     8521 gatgcgccag atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa
     8581 ggcatacccc catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa
     8641 taaaaacgga tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg
     8701 tggtgcattg cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt
     8761 cagtttgatt cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt
     8821 tccagccagg gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag
     8881 ccattggcgg aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc
     8941 tatgcttgtc tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaac
     9001 aatctgcaat ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa
     9061 attaaggctg atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa
     9121 tatacttatc ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc
     9181 gcggactaca gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac
     9241 gagatcagcg gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac
     9301 tggcagacta actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa
     9361 actcaaaaaa aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa
     9421 gccaaactgg cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat
     9481 gttggtggca gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg
     9541 ccagacattt ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt
     9601 gtgatttacg aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct
     9661 gtctccggta cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac
     9721 atcagcaccg cctcgatgcc atacctcact cgcccaggtc aggttcgcta taagatcatg
     9781 atgggccgtc cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa
     9841 gcttcctggg ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac
     9901 tatcagtctg cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt
     9961 gatgttactc actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg
    10021 gacggtaact ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt
    10081 acttttgctg gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat
    10141 gccagtgaca gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat
    10201 aaccagaact tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac
    10261 tgggatcgcg aggagcagac aaactacaac atcatgctct cgcactattt caatatgggc
    10321 agtattcgta atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc
    10381 gacaaaggca tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat
    10441 aacggtaact atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat
    10501 gacgcgactc actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc
    10561 tattacagcc atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg
    10621 caatacacct ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc
    10681 gcacttcacc gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt
    10741 gccgatgttc cggtagaagg taacggtgct gctgtttata ccaatatgtt tggtaaagcc
    10801 gtcgtttctg acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacaaactg
    10861 ccggaaaacg ccgaagcaac ccagtcggtg gtacaagcca cgctaactga aggggcgatt
    10921 ggctaccgca aatttgccgt catcagtggt caaaaagcga tggcggtgct gcgtttacaa
    10981 gacggcagcc atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc
    11041 cttgtcgatg atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt
    11101 gtgttctgga gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat
    11161 ctgtttaacg gcttgttact gccatgccag cataaaggca acgtcgcgcc ggttgttcct
    11221 gatgacataa agcctgtcat tcaggagcag acgcaacagg tgacacccac caatccccct
    11281 gtttctgttt cagctaacca ataacgtgat taaggaatga tccatgtctg atttactttg
    11341 ctctgccaaa ttgggtgcta cgacgctagc gttactgctt agcgctgcca gtttgtctgc
    11401 ccaggcttct gtaacgccag atcgtacccg tttgatattt aatgaaagcg acaaatcaat
    11461 cagcgtgacg ttgcgcaata acgatccgaa actgccttat ctggcccaaa gctggatcga
    11521 agatgaaaag ggtaacaaga tcagttcacc gttaactgtt ctgccaccgg tgcaacgcat
    11581 cgattcaatg atgaacgggc aggtaaaagt gcagggaatg ccggatatca acaaattgcc
    11641 tgccgatcgt gaaagcctgt tctacttcaa cgtgcgtgag atcccgccaa aatcaaacaa
    11701 agccaacacg ctgcaaatag cactacaaac gcgtattaag ttgttctggc gaccgaaggc
    11761 gctggagaac gtcagcatga aaaacccctg gcaatataag gttactttaa cccgcaacgg
    11821 tcaggaattt accgtcaata acccgacacc gtattacgtc attttcagta atgccagcac
    11881 acagaaaaat ggcaatccgg cagccggttt ctcgccaata gtgatgtcgc cgaaaacctc
    11941 tgcaccgctg aatgtgaaga tgggtagcgt gccggtactg acctatgtca acgactatgg
    12001 cgctcggatg ccgctctttt tcacttgcaa cggtaacagt tgccaggtcg atgaagagca
    12061 gagccgcaaa ggctgaggcc gggagagcca gtatgaaagg atcttgttct gtctgggcgg
    12121 taactgggtt actgatatgt tgtgttggta gcggctttgc agttgcagcg agtaagtcag
    12181 taagtatgaa tttacgggtg cttgtcgatg cccctcctcc ttgtacggta aatggtgcgg
    12241 cagttgagtt tggtaatgta ttcattaata agatcaatgg ggttgattat aaacgtccca
    12301 ttgactactc gcttgtttgt aacaatctgg caatggatga cctgcgtcta cagatgcagg
    12361 cgacaacggt cgtgattaac ggtgaaacag ttatcagcac ggggattcct gggtttggta
    12421 ttcgggttca gaaaagtagt gatcacacaa tactcgactt aacctctggc tcatggttgc
    12481 catttaattt ttcctcggga gttcccgttc tggaagctgt gccggtgaaa cagagtggta
    12541 caacgttagc ggcagctgaa tttaacgcca gtgccacaat tgtggtggat taccaatgaa
    12601 acggatctcg cttattttgc tgtggggctt ttgctcgatg gcactctcaa acgtctcttt
    12661 tcacggttat ttagtgcagc cacctaactg cacgattagc aacgctcaaa ccattgaaat
    12721 aacttttcag gatgtgctta ttgatgacat taatggcagc aattatgagc aaaccgtgcc
    12781 gtattccatt acctgcgata cggcggttcg cgacccatta atggaaatga ccttgagctg
    12841 gagtggcaca ccaagcgatt ttgataatgc tgcagtaagc agcaatatca ctggtttagg
    12901 cattcagctc aaacaggcag ggcagagttt tacgatcaat acgccacttg tggtgaatga
    12961 aaccgattta ccggtgttaa cggctgtgcc agtaaaaaag agtggtgtcg ttctgccaga
    13021 ggcggacttt gaggcctggg ccacgttaca ggtagattac caatgaagaa aaagcgtacg
    13081 ttgtttttta ttagtagcct gatgttgctg ggcagtggaa ccacaatagc aggagataat
    13141 ttgcacttta ccggcaattt aatcagtaaa tcgtgtacgc cagtgattaa tggtagccag
    13201 ttggcagaag tacattttcc tgccattgcc gccagcgatt taatgaacct tgggcagtca
    13261 gagcgagtgc cattggtgtt tcaattgaaa gattgtcata gttctacatt gtttaatgtc
    13321 aaagtaacgt tgacaggaac agaagatagt gctctacccg gctttctggc ctttgactcc
    13381 tcgtcatcag catccggcgc tgggattggt attgaaactg cggcaggcac atcggtgcca
    13441 atcaacaata caacaggcgt cacgctcccg ctgaatcagg gaaataacag cctaaatttt
    13501 aatacctggt tacaggcaaa gagcgggcga gatgtaacgt ccggagactt tagtgccaca
    13561 gtgacggcaa cctttgagta tttctgaaat gaaaaaatgg accataattt tcgcgagcct
    13621 aatgctgctc gttctgagtg ttgtcggcgc atctaagagt tatgctggag ataaacttat
    13681 gagtgcgagt tttgacagtg tgagagttta ttatgccatg gacaaggtaa ccggtgctgt
    13741 agcgagcagt gttttcaatg ttacggtgat aacgccaaag gaggttgcat atgggaaata
    13801 tgattctttt gcgtataaag gcgatacgtt gcgggttatt tcctggtctg ggtcgggttc
    13861 agctccaact ttggtactga ctgactttga tacaattaat aactcaaatt gccctggcat
    13921 agatacgaaa atttttcgtt gtgcatatat gacgtttaaa attaccgttg catctgatga
    13981 ttatggttgc ccttggatcg catcctttta ttcctataca gatttacccg gatttggaag
    14041 ctatacggcc cctacagtgc ataacacaat atgcccaacg ataccggtcg cttcatacga
    14101 tatctcctgg agtgaaaatt atgtcagcca taataaggcg ttacgaattc agtccacggg
    14161 gtcaacagtg accaccacgc tttcgaccta cttgatggaa ggcggcagac tctgtgatgg
    14221 cagtaatttt tctgataatg atggcagagg agcatactgc cgcgcggtat cagaattgtt
    14281 aacctttacc tcttacggtt gtgataaatc aacggtaacg gttacgccaa ctcgtcatcc
    14341 ggtaacggat aaagtactac acgatattgt ggtgaatgtg aataccagta gtgggcaacc
    14401 gattgattcg acgtgtcgtt ttcagtatgt attgaatgaa ctgtagttcg tagtattgct
    14461 ctgatgcgtc ttatcaggcc tacgaagtga attgcaattt attgaattat cacatatttg
    14521 taggccggat aaggcgttta cgctgctccg gcatttgctg ccagcattca gggcaactcc
    14581 ggcggcaacc actcttccac ttcaatcaac accaacaaag ccgcatcacc gccgtactct
    14641 ttcggtgcct ggtgaaatgc catcacatgc ggatgttgtg ccagccacag cggtgtttgt
    14701 tgcttcaaaa tatgcttccc gtgcccatgc atcacgcagg cacaaaacac atgttcgcgg
    14761 cgacaggcgg caatcaacgc gcccagttcc tgcttggcct gcagctgcgt cagaccgtgt
    14821 aaatccaaaa acaattccgg tgaataatcg ccgcggcgca gtttcttcgc ctcaaaatgg
    14881 ctgacatccg ggcgaacata tttcaccgga ccttcggtat ttaataacgg ctgaaactca
    14941 tcggagaaat aatggctggc atcagcctgc tcctggatca agcgtttcac cggcacttcg
    15001 ctgatttttt tacgctgcgg tcggtggaca atcgtgtcct gcttaatctt gcgagtcccc
    15061 gccatcaact ggcgaaacag agcctggtcc tcctcgctaa gtgttgtttt ctttttcatt
    15121 tatctgtctc tatttctaat tttcctacag tttacccgac tcgtagcaca atcgatcgtt
    15181 ttaaacgtat ttttcctcac tcaccgcgcg tgaatgctga tttatcgtca tcttcatggc
    15241 aaactagccg ccgaaattat tgcgatcatg ccctggagga atacgtggat aaaattttcg
    15301 ttgatgaagc agtaaatgag ctgcaaacca ttcaggacat gttgcgctgg tcggtaagcc
    15361 gcttcagcgc ggcaaatatc tggtacggtc acggcaccga taacccgtgg gatgaagccg
    15421 tacagctggt gttaccatcg ctctacctgc cgctggatat cccggaagat atgcgcaccg
    15481 cgcgtctgac ctccagcgaa aaacaccgta ttgttgaacg cgtgatccgc cgcgtcaacg
    15541 agcgcattcc ggtggcttac ctgaccaaca aagcctggtt ctgcggtcat gaattttacg
    15601 tcgatgaacg cgtgctggtg ccgcgctcac cgattggtga actgatcaac aataaatttg
    15661 ccggactcat cagcaagcaa ccgcagcata ttttagatat gtgtaccggt agcggctgca
    15721 tcgccattgc ctgtgcttat gccttcccgg aagcggaagt cgacgcggta gatatctctc
    15781 ccgacgcgct ggcggttgcc gaacagaaca tcgaagaaca cggtctgatc cacaacgtca
    15841 ttccgattcg ttccgatctg ttccgtgatt tgccgaaagt gcaatacgat ctgattgtca
    15901 ctaacccgcc gtatgtcgac gcggaagata tgtccgacct gccaaacgaa taccgtcacg
    15961 agccggaact gggcctggca tctggcaccg acggcctgaa actgacgcgt cgcattctcg
    16021 gtaacgcggc agattacctt gcggatgatg gcgtgttgat ttgtgaagtg ggcaacagca
    16081 tggtacatct tatggaacaa tatcccgatg ttccgttcac ctggctggag tttgataacg
    16141 gcggcgatgg agtgtttatg ctcaccaaag agcagcttat tgccgcacga gaacatttcg
    16201 cgatttataa agattaagta aacacgcaaa cacaacaata acggagccgt gatggctgga
    16261 aacacaattg gacaactctt tcgcgtaacc accttcggcg aatcgcacgg gctggcgctc
    16321 ggctgcatcg tcgatggtgt tccgccaggc attccgctga cggaagcgga cctgcaacat
    16381 gaccttgatc gtcgtcgccc tgggacatcg cgctatacca cccagcgccg cgagccggat
    16441 caggtcaaaa tactctccgg tgtttttgaa ggcgttacta ctggcaccag cattggcttg
    16501 ttgatcgaaa ataccgacca gcgttctcag gattacagcg caattaaaga cgtcttccgt
    16561 ccaggccatg ctgattacac ctacgaacaa aaatacggtc tgcgcgatta tcgcggcggt
    16621 ggacgttctt ccgcccgcga aaccgccatg cgcgtggcgg caggggcgat tgccaaaaaa
    16681 tatctcgccg agaaatttgg tattgaaatc cgcggctgcc tgacccagat gggcgacatt
    16741 ccgctggaaa tcaaagactg gtcgctggtt gaacaaaatc cgttcttctg cccggacccc
    16801 gacaaaatcg acgcgttaga tgaactgatg cgcgcgctga aaaaagaggg cgactccatc
    16861 ggcgcgaaag tcaccgttgt tgccagtggt gtccccgccg gacttggcga gccggtcttt
    16921 gaccgcctgg atgccgacat cgcccatgcg ctgatgagca tcaacgcggt gaaaggcgtg
    16981 gaaattggcg acggttttga cgtagtggcg ctgcgcggca gccagaaccg cgacgaaatc
    17041 accaaagacg gtttccagag caaccatgcg ggcggcattc tcggcggtat cagcagcggg
    17101 cagcaaatca ttgcccatat ggcgctgaaa ccgacctcca gcattaccgt gccggggcgc
    17161 actattaacc gctttggcga agaagttgag atgatcacca aaggccgtca cgatccctgt
    17221 gtcgggatcc gtgcagtgcc gatcgcagaa gcgatgctgg cgatcgtttt aatggatcac
    17281 ctgttacggc agcgggcgca aaatgccgat gtgaagactg atattccacg ctggtaaaaa
    17341 atgaacaaaa ccgcgattgc gctgctggct ctgcttgcca gtagcgctag cctggcagcg
    17401 acgccgtggc aaaaaataac ccaacctgtg ccgggtagcg cacaatcgat aggcagtttt
    17461 tctaatggct gtattgtcgg cgctgacacg ttgccgatac agtccgaaca ttatcaggtc
    17521 atgcgtaccg atcagcgtcg ctatttcggt cacccggatc tggtgatgtt tatccagcgt
    17581 ctgagtagcc aggtgagcaa tctgggcatg ggtacggtgc tgattggcga tatggggatg
    17641 cccgctggtg ggcgtttcaa cggcggtcat gccagccacc agaccggact ggatgtcgat
    17701 atctttctgc aactgccgaa aactcgctgg acctccgcgc agctcttgcg cccgcaagca
    17761 ctggacttag tttcccgcga cggtaaacac gttgttccga cgctgtggaa gccagaaatt
    17821 ttcagcttga tcaaactcgc cgcccaggac aaagacgtca cacgcatttt tgttaatccg
    17881 gcgattaaac aacaactttg ccttgatgcg ggcaccgatc gcgactggtt gcgcaaagtg
    17941 cgaccctggt tccagcatcg cgcgcatatg catgtacgat tacgttgtcc tgccgatagt
    18001 ctggagtgtg aagatcaacc tttaccgcca ccaggcgatg gttgcggggc agaactgcaa
    18061 agctggtttg cacctccaaa accgggaaca acaaagcctg agaagaagac accgcctccg
    18121 ttgccgcctt cctgccaggc gctactggat gagcacgtga tctaatggaa acgtttaata
    18181 gcctgtttat ggtttccccg ctgttgctgg gagttctctt ttttgtcgcc atgctggcgg
    18241 gatttatcga ctcgattgcc ggtggcggtg ggttactcac cattccggca ttgatggcag
    18301 cgggaatgtc tcccgctaac gcgttggcaa ccaataaact gcaagcctgc ggcggctcta
    18361 tttccgccac tatctacttt attcgccgca aagtggttag tttaagcgat cagaaactca
    18421 atattgccat gacctttgtc ggctcaatga gcggcgcact gttggtacag tacgttcagg
    18481 ctgatgtctt acggcagatt ttgcccattc tggtgatttg tatcggcctc tatttcttgc
    18541 tgatgccaaa gctgggtgaa gaagatcgcc agcgccggat gtacggttta cccttcgcgt
    18601 taattgccgg tggctgcgtc ggtttttatg acggattctt tggcccggct gccggatcgt
    18661 tttacgcgct ggccttcgtt acgctgtgcg gattcaacct cgccaaagcc acggctcacg
    18721 ccaaattact caacgcaacg tcaaacattg gcggtctgct gctgtttatt ctcggcggca
    18781 aagtgatttg ggcgacgggc tttgtgatgc tggtggggca gttcctcggc gcgcgcatgg
    18841 gttcacggct ggtgttgagc aaaggacaaa agctgatccg cccgatgatt gttatcgtct
    18901 cggcggtgat gagtgccaaa ttactttacg atagccacgg acaggagatc ctccactggt
    18961 tggggatgaa ctaatgaaca gtacacacca ctatgagcag ttgattgaaa tttttaatag
    19021 ctgctttgcc gatgaattta atacccgtct gattaaaggc gacgacgaac cgatctatct
    19081 tcctgctgat gcggaagtac cgtataaccg aattgtcttt gcccacggct tttatgccag
    19141 cgcaattcat gagatttcgc actggtgtat tgccggaaaa gcgcgccgtg agctggttga
    19201 tttcggctac tggtattgcc cggatgggcg tgatgcccaa acgcaaagcc agtttgaaga
    19261 tgttgaagtg aagccgcagg cactcgactg gttgttctgt gtggcggcgg gatatccgtt
    19321 taacgtcagt tgcgacaatc tggaaggcga cttcgaaccg gatcgtgtgg tgtttcaacg
    19381 gcgggtacat gcgcaggtga tggattacct agcaaacggc attcctgaac gcccggcacg
    19441 cttcattaaa gcactacaaa attattatca cacgccggaa cttacggcgg aacagttccc
    19501 gtggccggaa gcgctcaact aaagccagcg ccacgtttta caatgaggaa agaagatgat
    19561 cgcggagttt gaatcacgca ttctggcatt aatcgacggt atggttgacc atgccagtga
    19621 tgatgagttg tttgccagtg ggtatttgcg tggccacctg acattagcca tcgcagaact
    19681 ggaaagtggt gatgaccact ccgctcaggc ggtgcatacg accgttagcc agagtctgga
    19741 aaaagccatt ggtgccggtg aattgtcgcc gcgtgaccag gcgctggtga ccgatatgtg
    19801 ggaaaacctg tttcagcagg cgtcacagca gtaagttgcg gcgtctggag caccatgcct
    19861 gatgcgacgc tta
//
LOCUS       DAHBHC010000086        19802 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHBHC010000086 DAHBHC010000000
VERSION     DAHBHC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.086"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..145)
                     /locus_tag="M2A35_003932"
     CDS             complement(<1..145)
                     /locus_tag="M2A35_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCB7200164.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHL"
     gene            346..1344
                     /gene="yiaK"
                     /locus_tag="M2A35_003933"
     CDS             346..1344
                     /gene="yiaK"
                     /locus_tag="M2A35_003933"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCB7200165.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1356..1823
                     /locus_tag="M2A35_003934"
     CDS             1356..1823
                     /locus_tag="M2A35_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCB7200166.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            1941..2414
                     /gene="yiaM"
                     /locus_tag="M2A35_003935"
     CDS             1941..2414
                     /gene="yiaM"
                     /locus_tag="M2A35_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCB7200167.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            2417..3694
                     /gene="yiaN"
                     /locus_tag="M2A35_003936"
     CDS             2417..3694
                     /gene="yiaN"
                     /locus_tag="M2A35_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCB7200168.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            3707..4693
                     /gene="yiaO"
                     /locus_tag="M2A35_003937"
     CDS             3707..4693
                     /gene="yiaO"
                     /locus_tag="M2A35_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCB7200169.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            4697..6193
                     /gene="lyxK"
                     /locus_tag="M2A35_003938"
     CDS             4697..6193
                     /gene="lyxK"
                     /locus_tag="M2A35_003938"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCB7200170.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGGGFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            6190..6852
                     /gene="ulaD"
                     /locus_tag="M2A35_003939"
     CDS             6190..6852
                     /gene="ulaD"
                     /locus_tag="M2A35_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCB7200171.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            6845..7705
                     /locus_tag="M2A35_003940"
     CDS             6845..7705
                     /locus_tag="M2A35_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCB7200172.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEMMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            7699..8394
                     /gene="araD"
                     /locus_tag="M2A35_003941"
     CDS             7699..8394
                     /gene="araD"
                     /locus_tag="M2A35_003941"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCB7200173.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(8426..8527)
                     /locus_tag="M2A35_003942"
                     /pseudo
     CDS             complement(8426..8527)
                     /locus_tag="M2A35_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(8741..9481)
                     /locus_tag="M2A35_003943"
     CDS             complement(8741..9481)
                     /locus_tag="M2A35_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCB7200174.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQVDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            9605..10579
                     /locus_tag="M2A35_003944"
     CDS             9605..10579
                     /locus_tag="M2A35_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7200175.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(10576..11712)
                     /locus_tag="M2A35_003945"
     CDS             complement(10576..11712)
                     /locus_tag="M2A35_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCB7200176.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(11718..12041)
                     /locus_tag="M2A35_003946"
     CDS             complement(11718..12041)
                     /locus_tag="M2A35_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCB7200177.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            12224..12367
                     /locus_tag="M2A35_003947"
     CDS             12224..12367
                     /locus_tag="M2A35_003947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200178.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRPLTIE
                     TRD"
     gene            complement(12586..14124)
                     /gene="aldB"
                     /locus_tag="M2A35_003948"
     CDS             complement(12586..14124)
                     /gene="aldB"
                     /locus_tag="M2A35_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCB7200179.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADELTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(14289..15440)
                     /gene="yiaY"
                     /locus_tag="M2A35_003949"
     CDS             complement(14289..15440)
                     /gene="yiaY"
                     /locus_tag="M2A35_003949"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCB7200180.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(15629..17473)
                     /gene="selB"
                     /locus_tag="M2A35_003950"
     CDS             complement(15629..17473)
                     /gene="selB"
                     /locus_tag="M2A35_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCB7200181.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(17470..18861)
                     /gene="selA"
                     /locus_tag="M2A35_003951"
     CDS             complement(17470..18861)
                     /gene="selA"
                     /locus_tag="M2A35_003951"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCB7200182.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPYVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRVDKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(18959..19567)
                     /locus_tag="M2A35_003952"
     CDS             complement(18959..19567)
                     /locus_tag="M2A35_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCB7200183.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT         4770 a         5284 c         4979 g         4769 t
ORIGIN      
        1 aaagatgcgc caacggacaa ccgtttggat agttgctcaa aatctcaatc agcatcaacc
       61 cgcgaaacag actctggctt ccggctggac gctctttttc ctgcgccatc tcgttctctt
      121 tttttcccat cacttctttc cccattttgt cgcgtcctga tggtagcgca aagtgtgccg
      181 tagttcacga tctcgacaga taatttataa ccacttgatt tttatgtctt ttgaaattca
      241 tcaatcagat tgctttgtta aaaagcgatc gatatatttg aaatcaagtt tcgcatattg
      301 aaattttaag ccaaaaaagc gatcaaaaaa acaaggaagc ctgagatgaa agtgacattt
      361 gagcagttaa aagcagcctt taatcgagtc ttaatttcac gcggcgttga cagcgaaacg
      421 gctgacgcct gtgcagagat gttcgcccgc accaccgaat ccggcgttta ttctcacggc
      481 gttaatcgtt tccctcgttt cattcaacaa ctggaaaacg gcgatatcat tcctgatgcc
      541 caacccaaac gtataaccag cctcggcgca attgaacagt gggatgccca gcgttcgatc
      601 ggcaacctga cggcgaaaaa gatgatggat cgcgccattg aactggctgc cgatcacggt
      661 attggtctgg tggcacttcg taatgccaac cactggatgc gcggcggcag ctacggctgg
      721 caggcggcgg aaaaaggcta tattggcatt tgctggacca actccatcgc cgtaatgccg
      781 ccgtggggcg caaaagagtg tcgcattggc actaacccgc tgatcgtcgc cattccttcc
      841 acgccgatca ccatggtcga tatgtcgatg tcgatgttct cttacggcat gttagaagtt
      901 aaccgcctgg cgggccgtca gctcccggtc gatggtggct ttgatgatga gggcaatttg
      961 accaaagaac ctggcgttat cgagaagaat cgccgcattt tgccgatggg ctactggaaa
     1021 ggttctggca tgtcgattgt gctggatatg atcgctactc tcctttccga cggcgcatcg
     1081 gttgccgaag tcacccagga caacagcgac gaatacggca tttcacaaat ttttattgcc
     1141 attgaagtgg acaagcttat cgacggtccc acccgcgatg ccaagctgca acgtatcatg
     1201 gattacgtta ctaccgctga acgcgctgac gaaaatcagg ccatccgctt acccggccac
     1261 gaatttacta ccctgctggc cgaaaaccgc cgtaacggta ttaccgttga tgacagcgtg
     1321 tgggccaaaa tccaggcgtt ataaggagat aagtcatgat ttttggacat atcgcgcagc
     1381 ccaatccgtg ccgtttgccc gccgccattg aaaaggcgct cgattttctg cgcgctaccg
     1441 atttcaacgc cctggagccg ggcgttgtcg aaatcgacgg caaaaatatt tatgcgcaaa
     1501 ttatcgattt aaccacacgc gaagcggtgg aaaaccgtcc ggaagtgcat cgtcggtata
     1561 tcgatattca gtttctggcg tggggtgaag agaaaatcgg cattgctatt gatacgggaa
     1621 ataataaagt cagcgaatca ctattagagc agcgcgatat tattttttat catgacagtg
     1681 aacatgaatc atttatagaa atgataccgg gcagctacgc aattttcttt ccgcaggatg
     1741 ttcaccgacc aggttgtatt ttgcaaacag cctctgagat acgaaaaatt gtggttaaag
     1801 tcgcattaac ggcgctgaat taatcgaata atcgtctacg ccagaacgac tgggtaatgt
     1861 tattgctcta actatattaa taatttaggt tgctgcacat gcaacttgaa ttatgacaga
     1921 catagcctca aggaatagct atgaaaaaaa tacttgaagc aatactggcg attaatctcg
     1981 ccgtactttc ctgtattgtg tttataaaca ttattttaag atatggtttt cagacaagca
     2041 ttttatctgt agatgaattg tcacgttatt tatttgtctg gttaacattt attggcgcga
     2101 ttgtggcttt tatggataac gcccacgttc aggtcacctt tctggtggag aaactctccc
     2161 ccgcatggca acgacgagtc gctctggtta cccattcctt aatcttattt atttgtggcg
     2221 cactggcctg gggcgcaacg ctaaaaacaa ttcaggactg gagcgattat tcaccgatcc
     2281 tcggtttacc catcggcctg atgtatgccg cttgtctgcc caccagcctc gttatcgcat
     2341 tctttgaact gcgtcattta tatcaactca tcacgcgtag caattctcta acgtcgccac
     2401 cgcaaggagc ttaatcatgg ctgtgctgat ttttctgggc tgtctgttgg gtggtattgc
     2461 tatcggtttg cctattgcct gggcactgtt gttgtgcggg gcggcgttaa tgttctggct
     2521 ggacatgttt gatgtccaga tcatggcaca aacgctggtg aacggagccg atagcttctc
     2581 cctgctggcg attccgttct ttgtgctggc gggtgaaatc atgaatgcgg gcggcttgtc
     2641 aaaacgcatt gttgacctgc cgatgaaact ggtggggcat aaaccaggcg ggctgggcta
     2701 cgtcggcgtg ctggcagcta tgattatggc gagcctttct ggttctgccg ttgcggatac
     2761 cgccgccgtt gccgctctac tggtgccgat gatgcgcagc gccaactacc cggtcaaccg
     2821 ggcggcgggg ctgattgcct ctggcggcat tatcgcgcca attattcccc cttccattcc
     2881 gtttattatc ttcggcgttt ccagcggatt atccatcagt aagctgttta tggcgggcat
     2941 tgcccctggc atgatgatgg gcgcaacgct gatgcttacc tggtggtggc aggccagtcg
     3001 ccttaattta ccccgccagc aaaaagcaac gatgcaggaa atctggcact cttttgtctc
     3061 cggtatctgg gcgctgtttc ttccagtcat cattattggc gggtttcgct ccggtctgtt
     3121 tacccctacc gaagccgggg ccgttgctgc tttctatgcg ctgtttgtcg ccacagttat
     3181 ttaccgtgaa atgaccttcg ccacactttg gcatgtactc attggcgcgg cgaaaaccac
     3241 ctcagtggtt atgtttctgg tggcctcggc acaagtttcc gcctggctga ttaccattgc
     3301 tgaactgccg atgatggttt ctgatttact gcaaccgttg gtcgattcac cgcgtctgct
     3361 gtttatcgtc attatggtgg ctatcctgat tgtcggcatg gtcatggatt taacgccgac
     3421 ggtattaatt cttaccccgg tattaatgcc tttagttaaa gaggcaggaa tcgatccgat
     3481 ttatttcggt gtcatgttta tcattaactg ctcaatcggc ttaattacac cgcctatcgg
     3541 taatgtactt aacgttattt caggggtggc aaaactcaaa ttcgatgatg cagtcagagg
     3601 cgttttccct tacgtcctgg ttttatattc attattagtc gtatttgttt ttattcccga
     3661 tctcatcatc ctccctttga aatggattaa ttaaaaggaa aatattatga aattacgctc
     3721 tgtaacctac gcattattca ttgctggcct ggctgcattc agcacatctt ctctggcggc
     3781 gcaatcttta cgtttcggtt atgaaacctc acaaaccgac tcgcaacata ttgcggcgaa
     3841 aaaattcaat gatttattgc aggagagaac caaaggcgag ctgaaattaa aattgtttcc
     3901 ggatagcacc ctcggtaacg cgcaggcgat gatcagcggc gtacgtggcg gcaccatcga
     3961 tatggaaatg tccggctcga ataactttgc cgggttatca ccagtgatga acttgcttga
     4021 tgtccctttc ctgttccgcg ataccgctca cgcgcataaa acgctcgacg gcaaagtcgg
     4081 tgatgatctg aaagcctcac ttgaaggtaa aggactaaaa gtactggcct actgggaaaa
     4141 cggctggcgc gatgtcacca actcgcgcgc accggttaaa acccccgccg acctgaaagg
     4201 gctgaaaatt cgcaccaaca atagcccgat gaatatcgcc gcattcaaag tctttggcgc
     4261 taacccgatc ccgatgccgt ttgccgaagt ctataccggg ctggaaaccc gcactatcga
     4321 cgctcaggaa cacccgatca acgtcgtctg gtcagcaaaa tttttcgaag tgcagaagta
     4381 cctttctctg acgcaccacg cctattcccc gcttctggtg gtgatcaaca aagcgaagtt
     4441 tgatggctta agcccggagt tccagcaagc gctaatttca tctgcgcagg aagcgggtaa
     4501 ctaccagcgc aaactggttg ccgaagatca gcaaaaaatc atcgacggca tgaaagaagc
     4561 gggcgtggaa gtcatcaccg atctcgaccg caaagccttt agcgacgctc tgggtaatca
     4621 ggttcgcgac atgtttgtaa aagatgttcc gcagggtgct gatctactga aagccgtgga
     4681 tgaggtgcaa taaaccatga cgcaatactg gctggggtta gattgtggcg gtagctggct
     4741 gaaagccggg ctgtatgacc gcgaaggccg ggaggcaggc gtgcagcgcc tgccgctgtg
     4801 cgcattaagc ccgcagccag gttgggcaga gcgcgatatg gcagaactgt ggcaatgctg
     4861 catggctgtc attcgcgaac tgcttaccca ttccggcgtt agcggggaac aaattgtcgg
     4921 tatcggtatc tccgcacaag gaaaaggctt gtttttgctg gataaaaaca acaagccgct
     4981 cggtaacgcc attttatcct cggatcgccg ggcgatggaa atcgttcgtc gctggcagga
     5041 agatggcatc ccggaaaaac tctacccgct gacccgacaa accttgtgga ccgggcatcc
     5101 ggtatcgctg ttacgttggc ttaaagagca cgaaccggaa cgctacgcgc aaattggctg
     5161 cgtgatgatg acgcacgact acctgcgctg gtgtttaacc ggcgtcaaag gctgtgaaga
     5221 gagcaatatt tccgagtcca atctctacaa catgagtctt ggggaatatg acccgtgcct
     5281 caccgactgg ctggggattg ctgaaatcaa tcacgccctg ccgcctgttg tcggatctgc
     5341 cgaaatctgc ggggagatca ccgctcagac agccgtactg accggtctga aagcgggtac
     5401 gcccgttgtc ggcggcctgt ttgatgtggt ttccaccgca ctctgcgccg ggatcgaaga
     5461 cgaatttacc cttaatgcgg tgatgggcac ctgggcggtg accagcggca taacccgcgg
     5521 tttacgtgac ggtgaagcgc atccgtatgt ctatggtcgc tacgttaacg atggtgaatt
     5581 tatcgttcac gaagccagcc ccacctcttc cggcaacctc gaatggttta ccgcacagtg
     5641 gggagaaatc tcgtttgatg agatcaacca ggccgttgcc agcttgccga aggccggtgg
     5701 cgatctcttt ttcctgccgt tcctgtacgg cagcaacgcc ggactcgaga tgaccagcgg
     5761 tttctacggg atgcaggcca ttcacacccg cgcacacctg ttgcaggcca tttatgaagg
     5821 gggggggttc agccatatga cccacctcaa ccggatgcgc gaacgtttta ctgatgtgca
     5881 caccctgcgc gtcactggcg gcccggcgca ctccgatgtc tggatgcaaa tgctggcgga
     5941 cgtcagcggt ctgcgtatcg agctgccgca ggtggaagaa accggctgtt ttggcgcggc
     6001 acttgccgcc cgtgtcggca ccggagttta tcgcgatttc agcgaagctc aacgtgactt
     6061 acagcacccg gtacgcaccc tgctgccgga tatgaccgcc catcagcttt accaacaaaa
     6121 ataccaacgc tatcagcatc tcattgccgc acttcagggc tttcacgccc gcatcaagga
     6181 gcacatatta tgagccgacc acttctgcaa ctggcactcg accactcatc acttgaagcc
     6241 gcacagcgcg acgtgacgca gttaaaagac agcgtcgata ttgtcgaagc gggcaccatt
     6301 ctctgtttaa acgaagggct tggcgcggtg aaagccttgc gcgaacagtg ccctgacaaa
     6361 atcatcgttg ccgactggaa ggtcgccgac gctggtgaaa cgctcgcgca acaggcattt
     6421 ggcgcaggcg ctaactggat gaccatcatc tgcgccgcac cgctcgcgac ggtggaaaaa
     6481 ggccacgcga tggcgcaacg ctgcggtggt gaaattcaga tcgagctgtt cggtaactgg
     6541 acgctggacg acgcccgcga ctggcatcgt attggcgtgc ggcaggcgat ttatcatcgc
     6601 ggtcgtgatg cacaggccag cgggcaacag tggggcgaag ccgatctggc acgcatgaag
     6661 gcgctttccg atatcggcct tgagctttcc attaccggcg ggattacccc tgctgacctg
     6721 ccgctgttta aagatattcg cgtgaaagcg tttattgccg ggcgagcact ggcaggctca
     6781 gcaaatccgg cgcaggtggc tggcgatttc catgcgcaaa tcgacgctat ctggggaggc
     6841 aagcgtgcgt aatcatccgt tagggattta tgaaaaggcg ctggcgaaag atctctcctg
     6901 gccggagcgg ctggtgctgg caaaaagctg cggttttgat tttgtcgaaa tgtcggtgga
     6961 tgaaaccgac gaacggcttt cacgtcttga ctggagcacc gcacaaagga cttcgctggt
     7021 tgccgcgatg atcgaaacgg gtgttgggat cccgtcgatg tgcctgtccg cacatcgtcg
     7081 ctttcctttt ggtagccgtg acgacgcggt acgccagcga gcacgggaga tcatgagcaa
     7141 agcgattcgt ctggcacgcg atctcggcat acgcaccatc cagctggcgg gctacgacgt
     7201 ctattacgaa gaccacgatg aaggcacccg gcaacgtttt gctgaagggc tggcgtgggc
     7261 ggtcgaacag gcggcggcat cacaagtcat gctagcggtg gagatgatgg acaccgcgtt
     7321 tatgaactcc atcagtaaat ggaaaaagtg ggacgagatg ctcgcctcgc cgtggttcac
     7381 cgtctacccg gacgtcggca acctcagcgc ctggggcaat gatgttcccg ccgaactgaa
     7441 actgggcatt gaccgtatcg ccgcgatcca cctgaaagac acccagccag ttaccgagca
     7501 aagtcccgga cagttccgcg acgtgccgtt tggcgaaggc tgcgtcgatt tcgttggcat
     7561 cttcaaaacg ctgcataaac tgaactatcg cggttctttc ctgattgaga tgtggaccga
     7621 aaaagccaaa gagccggtgc tggagattat tcaggcgcgg cgttggattg aagcgcgtat
     7681 gcaggaggct ggatttatat gttagagcaa ctgaaagccg acgtgctggc ggcgaatctg
     7741 gcgcttcccg ctcaccatct ggtgacgttc acctggggca atgtcagcgc ggtagacgaa
     7801 acgcggcaat ggatggtaat caaaccttcc ggcgtcgagt atgacgtgat gaccgccgac
     7861 gatatggtgg tggttgagat agccagcggc aaggtggtgg aaggtagcaa aaagccctcg
     7921 tccgacacgc caacgcatct ggcgctttac cgtcgctacg ccgaaatcgg tggcattgtg
     7981 catacccact cacgccacgc caccatctgg tcgcaggccg ggctggatct ccccgcctgg
     8041 ggcaccacgc acgctgatta tttctatggt gccatcccct gcacgcgaca gatgaccgca
     8101 gaggagatta acggcgaata tgaatatcag accggcgaag tgatcattga aaccttcgaa
     8161 gaacgtggca ggagtccggc acaaatcccg gcggtgctgg tgcattctca cggcccgttc
     8221 gcatggggta aaaacgccgc cgatgccgtg cataacgccg tagtactcga agaatgcgcc
     8281 tatatgggcc tattctcgcg ccagcttgcg ccgcagctcc ctgcgatgca aaacgaactg
     8341 ctggataagc actacctgcg taagcatggg gccaatgcct attacgggca gtaatccctc
     8401 acgccggggc ttcatcgccc cggccctacg aattgatatg ttccttgctg taacgccgct
     8461 tccacgctgc tggagttaaa ccggtgtgct tgcgaaaaat ctgccggaaa tagccaacct
     8521 cattaaaccg tattgcatgg agcaggccga caacgtcgag tcactgcata acctgttgct
     8581 gcccgccgat gcgccattta cgacatttga aggcaaggga ttgttcagcc ataagatcct
     8641 gattcagacg ccaggttccc ggtatgaaca gagcaatcca gagcaacaac ctttgtggca
     8701 ttacgacgga gcaccagccg caccgacatc caccggtgaa ttaaaaacgc cagctcacgc
     8761 cggtgacaaa ggtaaaactc tcattacgat caatcatcgg gctgtcggta atctcttccg
     8821 gcagctcgct gtatccggcg ctcgccatca atactacgtg ctctcctatc gggtattttg
     8881 ccgtcaggct gacatagggc acccaggcat cctgcgcgga ataacttgcc agaccgctac
     8941 gacgggactc actctctgaa atgccatagt aatagtcact gaaattctcg tcataataga
     9001 gaacgcccag cgcaggtgtc agcgacaggc gacctatctg cattttgtgg aaaaccgata
     9061 gctcccccac ccagccgttg ctgttatcca gaacgtccgc agctgcagag gctttcacgg
     9121 ttccccaacg ctcgtggtga taccaggcaa cccccgccat agccgtacta tcacgcttat
     9181 caacttgttg catggcatga tcgtcattat cggcagggtc aaattccagc ggcatccagg
     9241 atgcggtcag gctaagttca tttttttcac tttgcgacag aataaaacct aacgtggtct
     9301 gacgaacata aaaagaatca ccttcataac taatcagggg aattgccttt gtattttcat
     9361 tataaccgcg ataaggcgat tcattataag ccgcaccggc accaatggat aattcagaag
     9421 cagttgcgct tgccataaaa ggcaacgcaa ataacgccac aatattgcgg ttaattaaca
     9481 tgtccggtat tccattttaa aataagataa aaaagagtcg gcatagtaat gcttacaatt
     9541 tctccggtgc aagattcata tttatatagt aatatataaa attatatata attgggctgt
     9601 tgcgatgaca aaattacaac ttaagtaccg ggagttaaaa attatctcgg taatcgctgc
     9661 cagtgaaaat atcagccatg ccgcgactgt acttggcatc gcacaggcca acgtcagcaa
     9721 atatcttgct gattttgaat caaaagtggg tttaaaagtc tttgaccgaa ccacccggca
     9781 gctgatgctc acaccttttg gcaccgcgct gttaccgtac atcaacgaca tgctggatag
     9841 aaacgagcaa cttaataatt ttattgcaga ttataagcat gagaaacgtg gtcgggtcac
     9901 tatctacgcc ccaaccggca taattaccta tttatccaaa catgtaatcg ataaaattaa
     9961 agatatcggt gatatcacct tatcgctaaa aacctgcaat cttgagcgta atgcctttta
    10021 tgaaggcgtc gaatttcctg atgattgtga tgttttaatc agctacgctc caccaaaaga
    10081 tgaatcgctg gttgccagtt ttattaccca atatgccgta accgcttatg ccagccagcg
    10141 ctatcttgag aaacatccta ttagccgtcc tgacgaactc gaacatcact cctgtatttt
    10201 gatcgactcg atgatgattg acgacgcgaa tatctggcgc ttcaacgtcg ccgggagtaa
    10261 agaggtgcgg gattatcgcg tgaagggaaa ttacgtttgc gataacacgc aatcggcgct
    10321 ggagctggca cgaaatcacc tggggattgt gtttgcgcca gacaaaagtg tgcagagcga
    10381 cctgcaagac ggcacgctgg tgccctgctt tcagcacccg tatgaatggt ggctggatct
    10441 ggtggctatc ttccgtaaac gggaatacca gccctggcgg gtccagtatg tgctggatga
    10501 aatgctgcgt gaaattcgcc accagcttgc tcagtcgcag caactgcggc ctgaacaagc
    10561 ggcggaaagc gaagattaat gatcgagata aaggtaatgc acccaactgg tcatgcgtaa
    10621 cagcactttg cgcatcaccg agacatggct gaaatgatca gagtagaccg ccacctgggc
    10681 gtaaataccg tctggtaaag cgctcaaatc agtgtgctca tccagttcaa tggtcgcgat
    10741 aacgccatct gaacccggcg ctgtgtttaa cgtctgtaag gtgccggtcg actgataagc
    10801 tccgccggga acagctggac taatggctgc cagcttgccg ctgaatacct tacctggcag
    10861 agcgttaaat accacttccg catcatcgcc aggggccagt cgcagcaagg agttctgacg
    10921 gaactgcgcc acaatttgtc gtttctgatc gggtataaac accatcaccg gacgtagcgg
    10981 cagcgacgcg gcataggtac ccggacgaat cagcacctgg gtcacataac catcgctcgg
    11041 cgcacgcact atcgtctgct caaggttata ttttgcttcc gcgagctgcg ctttcaggct
    11101 ggcgatttga gaatgttcac ccaacaccag gctatccagc tggctctgga tctgtttttg
    11161 ttccgccgcc gatgacttta cggaggcttc ctgcgccaga taattttgcc gcgccacatc
    11221 aatatcgcgt tctgaaaacg ggtttacttt cgcctggctg ccgcgtgcgt aacgctgata
    11281 ctctttagcg aatttatccc gcgtggcctt tgcctgctga gtattcgccg ccatctcatc
    11341 taactctgcg cccaacgccc gctgtttatg ttctgcggta acgatatccg ccatcagccg
    11401 atccacccgc gcctgataac gcgtcgggtc cagtcgaaat agcacctcac cttttttaat
    11461 cagcgtattt ttcttatccg tcacttcgat caccacgcct gtcacctgtg ggacaacagg
    11521 aatagaaata accgcttttt gcgctttgaa ggtatacgga tggttatagt tcattaacag
    11581 aattaaacca ctgacaataa atatgccacc cagggccgct gtgggaatgg tccatttatt
    11641 tacaggaatt ttgaagatct taaacattgc ccatgcgaaa gccacatagg tcaaaataat
    11701 cagtaaatcc ataagtatta ctcggcagaa gaggatttga tgtcggcgag ctgtttctcc
    11761 agcccggcaa tacgctgttg tagctgcaca acggaggagt catggttttg cattccccat
    11821 ccccgctccg ggcggtaaag cgtggcccaa atccacaaaa atggccagat aacatgtaac
    11881 gtaaaaagac tcacccaacc agcaacatga atggcgtcgg catgaggatg attacgtttt
    11941 ttagcaatca ggtagggaat atcatgtaaa ataataatcc catagaagat caccagaaat
    12001 acaaaaataa gcactcccag tgcaaaatag tccaggaaca tatttccctc agagaatata
    12061 aaaaagaaca attaacgcat attatgaaat gccatgatgc agtgcaattt ccttatttat
    12121 ataacgacat attgtttttt atatactttc tgaagtgtgc tacctggctt ttcaacaggt
    12181 cgagacttat catattgaaa taatggaact attggccaaa ctaatgaata actccagtaa
    12241 aacatccaca gtacagatta agcgtattaa accttcaatt atctaccgtt tattgctgat
    12301 tggcctcgga tcaccaatgg tgatttacgg cctgattcgc ccgctcacca tcgaaacgcg
    12361 agattaaact cctgacaaaa cacgccccag aaacgcactt ctgttggcgt agtctgggtt
    12421 attgcgcacg taggtttaaa acgtaaattg taacaacgtg cgctttgttt atgccggatg
    12481 cggcgtgtac gccttatccg gcctacggtc aggttcccgt aggcatgata agacgcgtaa
    12541 gcgtcgcatc aggcaatgaa tacccaatgc gaccagcttc ttatatcaga acagccccaa
    12601 cggtttatcc gagtagctca ccagcaggca cttggtttgc tggtaatgct ccagcatcat
    12661 cttgtgggtt tcgcgaccga tacctgattg tttgtagcca ccaaacgcag catgtgccgg
    12721 gtaagcgtga tagcagttgg tccacacgcg cccggcctga atgccgcgcc ccatcttata
    12781 ggccagatta ccgttacgac tccagacgcc tgcgcccagg ccatattgcg tatcgttcgc
    12841 cagctccagc gcttcttcca tcgttttgaa ggtggtcacc gccagcaccg ggccaaaaat
    12901 ctcctcctgg aacacccgca tattgttctg accaaacaga atcgtcggtt cgaggtagta
    12961 gccgtctttc agttcacctt ccagcagctt gcgccgcccg ccggtgagct cgtctgcgcc
    13021 ctctttttta ccgatatcaa tgtagttgag gatggtttcc agttgcccgt gagaaacctg
    13081 cgcgcccatt tgcgtcacgc tgtcgagtgg gttaccgctg cgaatgcttt cgacacggcg
    13141 gatggcgcgt tccataaagc gttcgtagat tgactcctgc actaaggcac gactcggaca
    13201 ggtgcaaact tcaccctggt taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa
    13261 aaaggcatct tcttcatcca tcacatcagc aaagaagata tttggcgatt tgccgcccag
    13321 ctccagcgtc accggaataa tgttttgcgt ggcgtattgc ataatttgtt ggcccacttc
    13381 cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat attcgccaat
    13441 ttccccacct gcgccgttga ccacgttcac cacgcccggc ggcagtaaat caccgacgat
    13501 ttccattagc agcagtacag aaagcggggt aagacgtgcg ggtttcagca ccacacagtt
    13561 gcccgccgcc agcgcgggag ccattttcca gctcgccatc agcagcggga agttccacgg
    13621 gataatctgc cccaccacgc ctaacggttc atggaaatga taggccacgg tttcgctatc
    13681 aacttcactg atcccacctt cctgcgcccg aatacacgag gcgaaatagc ggaaatggtc
    13741 aatcgccagc ggtacatccg cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt
    13801 ttcagctgtc gctaacagct cgaggttttg ttccattcga tcggcaatct taaacagaat
    13861 cgccgcgcga tcctgcaccg aggtgtgcgc ccatttatct ttcactttgt gcgcagcatc
    13921 cagcgccaga tcgatgtctc gtttgcccga agacgccact tcgcacagca gctgcccggt
    13981 caccggcgtc agattctggt aatactcgcc gtcggcaggg gctacccatt cgccgccaat
    14041 aaagttgtca tagcgggctt ttaacttgag ggggaaacca tactcgccgg gcttaatctg
    14101 tgctgaaggg ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta
    14161 gacgtgactg gcgaaatctt cgccagtcgg taacagcttt acgacagcta tcacgaattt
    14221 acgggcaagg gttacagaga tagttgaaaa agcggctgac aatttgccag ccgttgtgga
    14281 aatgatgatt acatcgctgc gcgataaatc gccacaattt cttcgtgagt tgcctggatc
    14341 gggttagtaa aaccacaggc atctttcagg gcattagtcg ccagaaccgc gaaatcttct
    14401 tctttcacgt tcaggtcgcg taggcctgcc gggatatcca ctttcttcgc cagttcacgg
    14461 atggcgttaa tgcaggcttc agcaccttcc gcatcgtttt tacctgtcac gttcacgccc
    14521 attgcagcgg cacagtcacg cagacgtgcg gcggcgactt tgctgttgaa tacctgaaca
    14581 tgcggcagca aaacggcgtt acatacaccg tgtggcaggt tgtagaaacc gcccagctgg
    14641 tgcgccatcg catgaacata acccagagaa gcattattga acgccatacc ggcgaggaac
    14701 tgggcataag ccattgcttc acgcgctttc gcattactgc catcttcaac ggctaacggc
    14761 aggttttcgg caatcatggt cacggctttc agtgcacaag cgtcagtgat cggcgtggcg
    14821 gcaatagaaa catacgcttc gatagcgtgc gttaaggcat ccatacccgt tgcggcggtc
    14881 agtgacttcg gcataccaat catcagagag gagtcattga cagaaagcag cggagtgaca
    14941 tgtttatcaa caatcgccat tttgatatga cgcgcttcgt cagtgatgat gcagaaacgg
    15001 gtcatttcag aggccgtacc cgccgtggta ttgatggcga tcatcggcag ctgcggtttt
    15061 gcagagcggt caacgccttc gtaatcgcga atatcgccgc cattggctgc caccagcgca
    15121 ataccttttg cgcagtcgtg tggagaacca ccgcctaagg agatcacgct atcgcaatta
    15181 ttctctttaa gtaatttcaa acctgcggcg acgttttccg tggtggggtt aggttgggtg
    15241 ccatcataaa taacgctaaa aatattgcgt tcttccagtg ctttttgcac atcgcccgcc
    15301 atacctaatt tcgttaacat actgtcagtg acaattaagg tacgggtaaa tccataatct
    15361 gccatcatat tcattgcatc agtcaatgaa tcagcgccga tgacattcac agaaggaata
    15421 aagaaagttg aagatgccat aacacactcc tgaaagtgta aaaaagggtg ggcagcttac
    15481 tgaggatttt cattcgaaaa catgatcatt attattgatt atcaaaatta atctaataaa
    15541 aagcacattg tattcattaa acaacgtgct tttttaaaat cataagaaat ttgtattaat
    15601 ttattttaaa aattaattaa tcatttcctt atttttccgg aaataataat gcgtcgcgca
    15661 ataaatgatc atttccacga cgacgcgtaa agccaatgcg gtcaaaatat tccagaatct
    15721 gaattgccag ctttcggcct acgcctaagc gatcgcggaa atccgccgcg caggttgaac
    15781 cacactcctg atcgagatcg cggatcatat tggcaaattc gacaatccga tcgttacggt
    15841 aataacgatc tttgacgatc gcggtaatta ttccttgctg cgccgcctgg cgtagcgtca
    15901 ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag atcacgcacc caccacggct
    15961 cgtcaccaaa cagcggctct gctttttgcc agatggcctg ctgctcttcg ctgaagcccg
    16021 ctttgtgatc cggcagatgc agccaaccgt gatgactgag gatgtcgccg ctttcgcgca
    16081 tcttttcaat cagcaacagt actagcgctt catcttccat tggcaacgcc atacgtcgca
    16141 gacgttcgcg cccaggccca ggttcatcgc gatgttgctc atgataagtc gctaatgtat
    16201 cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt caacaagcta taaccagcct
    16261 gaatatagcc aggctgttgc agcaattcgc gcatcccttc gccgttgagc tggcgcgccc
    16321 aggcgaaatc cgcaaggtta accgcgccgc gttccagatg aacagataac gcatcggcat
    16381 cgctctgcgc ccgtgcaaga gacgccagcc attgcagata ttccggctta cgtttaccgc
    16441 gacgcggcgg gttaagcatc accacacgcg cccccgccag cgtgttgcgg gccgagatat
    16501 cgcgcagcac taagcgatcg ttatccgcca gccacagcgg cgtgtcgaac accagctcgg
    16561 caaggttatc ttccagcagc gaaacgcgtc ccgtaacgtg gctggcggcg tggtgaatat
    16621 gcagcggctg ccactgggtc agcggtgtat gggtttgaag ctcgacaatc acccgtgtga
    16681 acggctctgg cggcacatcg gcaagcagcc agtcgccacg gttaatctgc tctttttccg
    16741 catcacccgc gatgttaagc gcgatacgct gcccggcatt ggcggtttct gttggctggt
    16801 tttgcgcatg cagcgcacgt acacgcatcg gtttatttac accagtcagc cagagtgaat
    16861 cgcctacctt cacttccccg cttaacgccg taccggtgac gaccagcccg gcacctttta
    16921 cggtaaatgc gcggtcaata gcgaggcgga aactatgttg gctggcgtgc tcgcgttccg
    16981 gcaactgaag cagatgctcg cgcagggcat caattccccg accttcggtt gctgcggtga
    17041 taaacagttt tgcctcagca aaaccgtatt cccgcagcac ctcctttacc tggcgttcaa
    17101 cctcattaac acgcgcttcg tccacgcgat cggctttggt cagcgccact gtcagcatcg
    17161 ggttaccggt cagctgcaaa atcgccagat gctcacgggt ctgtgccatc acgccgtcat
    17221 cgcacgccac caccaacagc gcgtgatcga taccaccaac gcccgccagc atgttggaaa
    17281 gaaacttttc atgaccgggt acgtcgataa aaccaggcac gcgaccatcc ggctgcggcc
    17341 agtaggcata gccgagatcg atggtcatgc cgcgcttttt ttcttccggc agacggtcag
    17401 catttacgcc agtaatcgcc tgcaataagg ttgttttgcc gtggtcaacg tgtccggcag
    17461 tcgcaataat catttcaaca acatctccaa aaaccgttgc tcatcttcaa ggcagcgtaa
    17521 atccagccac aatcgtccgt cataaatacg accaatcacc ggcactggca attcacgcca
    17581 gcgggcggct aatgactcaa ggtggctacc gcgtccatca tggggtgtaa acgttaatgc
    17641 cgcgctcggc aggcgatcaa ccggcagcga accactgcca atctgcgaaa gacatggcat
    17701 aacctgtacc gcaaactccg cgccgtaatg tgcggcaagg ggggcctgta aacgttgtgc
    17761 ctggatttga atgacctctg cgctgcgggt aagcaggcgc agggtcggta atttttcact
    17821 cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc agcgccgcga gggtcatttt
    17881 atccacgcgt aatgcacgct tcagcgggtg gctttgcagg cgggcgatca tctctttttt
    17941 accaacaata attcctgcct gcggcccgcc taacaacttg tcgccggaga aactcaccag
    18001 actgacgccc gccgcaatca actcctgcgg cattggctct ttcggcaaac cgtactggct
    18061 aagatcgacc agcgagccac tgcctaaatc agtcactacg ggaacatcca gctctttgcc
    18121 gagcgccacc agttccgctt catctatcgc tttggtgaac ccctgaatgc tgtagttact
    18181 ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc gcctgacgat aatcattcgc
    18241 gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag cctgcctgac gcataacata
    18301 gggaatacga aacgcgccgc caatctccac cagttcgccg cgagaaacca ccacctcttt
    18361 cccgctggca gtggccgcca acatcaataa caccgccgcc gcattgttat taacgataca
    18421 ggcatcttcc gcccccgtaa tgcggcacag cagttgcgcc agcgcccgat cgcgatgtcc
    18481 gcgtccggcg tcgtccagat cgtactcgag ggtcactggc gaacgcatag cctgcgcaac
    18541 ggcttccacc gcggcttccg cctgtaaagc tcgtccaagg ttggtatgca gcacggttcc
    18601 cgtcaggttg atcaccggac gcagcgcgct ctgcgcttct ttcgtcaacc gggcatcgac
    18661 ttcttgcgcc cagttttcac accacgcagg cagcgtctgg ctgccacgaa tcacttctcg
    18721 cgcttcgtcg agcatctgac gcaacaattc caccacgcgg gtgtgaccat aagtatcacg
    18781 caaagaaagg aaggagctat cgcgcaataa gcgatcaata gccggaagtt gactatagag
    18841 ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa ggccctctca cacggagaag
    18901 ggcgtttaac ataaccacgg attgtaacgt gagatgggtc aggaggacat atcgcgcatc
    18961 aagcctttgg cggttcggtg cgagcaaaac tttcgcggct aaacaggttt tcgaccagtt
    19021 tgactaagtg tgggcggtcg acacaccaac ctggcgcaac gcggcggaaa ttgagatatc
    19081 cgacggcaca ggcaatggcg atagtcgcca gattgaccgt atcggttttg agtgtgccat
    19141 cgaccagata tccttccagc acatccagac tgcggttgat tttctcccgc tggcgtaaca
    19201 attcatcttc agactgctgc gccgctggac gcgcctgttc acgcaccgat accagcccgg
    19261 catccataat gccatccgcc agtgcctcaa ttttgcgcac ccgcaacgac tccagcggat
    19321 cgcgcggcaa catcgccgga gcgacattca ttaattcaat atattcagcg atgatcggcg
    19381 aatcaaacca acattcgccc tcttcggtca ccagcaccgg cacttttcct aacgggttaa
    19441 attgcgccac gccgttgtcc gcgttatagg gcagttcatt aataaattcg aaagttatgc
    19501 ccttttctaa caacagaata gaaagtttgc gtacaaacgg gctggtgtag ctaccgacga
    19561 gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta tggcctggtg atgggaaaaa
    19621 gggcagaaaa tgttgatggt ttgttacttc caaataaatc acatatttat catggtgata
    19681 taaatatttt cctaattgtt tctatctgat ggatatctca cttaaggctt tcttataaat
    19741 ctgtagggtt ttgcctggaa gcagacaaat aacccgataa aacaaggatg agcagatgag
    19801 cg
//
LOCUS       DAHBHC010000087        19680 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHBHC010000087 DAHBHC010000000
VERSION     DAHBHC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.087"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(35..1810)
                     /locus_tag="M2A35_003953"
     CDS             complement(35..1810)
                     /locus_tag="M2A35_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200184.1"
                     /translation="MIINELAYKVTIKADEFLNGKRKVKQEAQDLKNEIERSSSAIER
                     STTSSMSKTAKETDKAAVAMKGLTASFNGFLGLSTRFVAVGGVLTAVAIGIHRAFEST
                     SESIVRASNMGRMLGTSASNVLGTQYGFSRIGQNGGAFLGAQMSARMALANIEDPTIF
                     GGLTPETQNLLTTGARTGIDISKLGGKSEDALAEFQKYGKNHNEKQLMQVLSAFGYDP
                     NLAGDIKSGKAVQMVSEEEKRWQMTKEQEEAQRNILATTKALDSQFAQVKQELMATFG
                     PEVLKAEQEFLEWLKNNKGDIVGFFRNLSTGIDGFVKAVGGAGNALGILAAMALLSGK
                     GLGSKLGLIGAVGIFGQMVQDQYKDVPDEMRPFAFQQHWLERLIGMDYDDPNKEIPPN
                     KNGVNRPDRNNNPLNLKATGNRRRDKDGFAQYFDPEEGWEAARNQLTLYYKRDKLDTL
                     QGIISKWAPSSENNIKAYIDQVSKSMGVGANDKLNLSDPEVMAKLSAYMARHEGYPDW
                     KRGLDYGNPTKNSNAAYYQTQQRLANGSANQSYAQSVVNNYNSQHIGEVKVIAKTDQS
                     SKLTEGFKELNRRSSVNQAFSSGVR"
     gene            complement(1807..2010)
                     /locus_tag="M2A35_003954"
     CDS             complement(1807..2010)
                     /locus_tag="M2A35_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200185.1"
                     /translation="MEEETLPAKLVSPVNVTPSMNAIICSGKATYTDLQERLSVRDMY
                     NLLEIISVESFNKRVWNKHQEQR"
     gene            complement(2022..2429)
                     /locus_tag="M2A35_003955"
     CDS             complement(2022..2429)
                     /locus_tag="M2A35_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200186.1"
                     /translation="MARKEIPFIVEDDNRDNGKEFIITEMSAWDADELAQDLFRSMGE
                     SGFSGIPADVIAMGCAGLATLGLNVISAASPEVARKLRDRLMSTVQIVITHEGSRQVR
                     TVKPVDFEEVSTIRQVMDKVFKVNFDFLTIAGE"
     gene            complement(2429..2863)
                     /locus_tag="M2A35_003956"
     CDS             complement(2429..2863)
                     /locus_tag="M2A35_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200187.1"
                     /translation="MPIDITSANSKLRIVVPAYYPGGFDVDDYAADNMFETGALQNKE
                     DMMSADGKYHAGFIFNPTEFTINLMPTSNAGSLIDDWYAAERTAISAFQCNAVLTVPA
                     LGAKWNFVNGVLYTWTPTPPGRRVLQPRPAVFHFESVTRSTI"
     gene            complement(2875..4362)
                     /locus_tag="M2A35_003957"
     CDS             complement(2875..4362)
                     /locus_tag="M2A35_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCB7200188.1"
                     /translation="MTIPLSTDFNITPNVVTPAGSAVDANGLMLTDNELIPVGAVQSY
                     YSSSDVSALMGSESKEFLAAQQYFNGYENSSVIPGELLMYRIVTSPVAGYLLSGNLKG
                     VTLATLKAIPAGTITLSIDGESTTSTSIDLSTATSFSDIASKLQVGIGASKVVVEWLP
                     IANRFIIRSATAGAESEVSYASAGALATGLLLTQDSAAIVSPGSDAVTLTDTMNNIIN
                     VNQNWILFNSLVELTDDQKTELCAWASSSKNRFGYVVHDTTSAGTVANNANCFVQKVV
                     VANGYENIFPVYGTYLYSVTALAYAASVDFARTNGRISFKFRGFSGLAPNVSDLATAQ
                     ALKSNGYNFYGSYSLNKTMAQYASDGAITGKFVWLDSFINQVWINANLVSAFANLFTN
                     NQSYPFNETGYGAVSAAVIDVAEQALNFGAIQRGVKLDNAQTRIVNNTVGKDISSTLY
                     SQGWFLYIPTQSGSARIERDLKGVIFYYVDGQLIQSITMSSTAIL"
     gene            complement(4359..4667)
                     /locus_tag="M2A35_003958"
     CDS             complement(4359..4667)
                     /locus_tag="M2A35_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200189.1"
                     /translation="MRYTEVRQADIQVDIYGQGAGDRAIALETTFTSGYGYDVIKAID
                     ARLAPLYSSPAIQAPMIDAESQWQERWTLTLSLQAHITVSFPQDYFDKAEITLQQVDI
                     "
     gene            complement(4865..5236)
                     /locus_tag="M2A35_003959"
     CDS             complement(4865..5236)
                     /locus_tag="M2A35_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200190.1"
                     /translation="MNLRQIANNAITSINPNIPAILKKYAGETIGPGRKPIPSYLPDQ
                     HVTIQLQPISRGDMQHVDGLNIQGLAKVIYVNGNYFSVQRELEQGGDIFVINGEQWLV
                     VEPVELWPNWCRLIAVLQVST"
     gene            complement(5211..5657)
                     /locus_tag="M2A35_003960"
     CDS             complement(5211..5657)
                     /locus_tag="M2A35_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200191.1"
                     /translation="MDKVMDALDEVEKQFSNIQLKVGFLEGATYGGVIPVPMVAATNE
                     FGNPAHNQPPRPFFRNAIAEHEEEWKEQAALLIERGDDIEEVFSMLGTIIAADIQQSI
                     RSLDSPPLSPVTIARKGFDNPLIDTKNMLDSVSYEVSEIESQTDSQ"
     gene            complement(5657..6088)
                     /locus_tag="M2A35_003961"
     CDS             complement(5657..6088)
                     /locus_tag="M2A35_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCB7200192.1"
                     /translation="MGVVIFDPSAFKLRYPEFSSVDDALLQQYFTQATIYLDNTDSSR
                     VSDLAVRSMLLNMLVAHIACLYSGANGQSPSGLVGRIDSASEGSVSVHADMPGVTANS
                     AWYMQTKYGADYWNATAPFRTFQYISGHSPSNYPYGYYRRY"
     gene            complement(6101..6283)
                     /locus_tag="M2A35_003962"
     CDS             complement(6101..6283)
                     /locus_tag="M2A35_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001631271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200193.1"
                     /translation="MDKFIETYKDAAYIQSNAIFIQKDIRSLNAQGKDLKESKTGLEG
                     LDPENPAPGVKKADEK"
     gene            complement(6452..7492)
                     /locus_tag="M2A35_003963"
     CDS             complement(6452..7492)
                     /locus_tag="M2A35_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCB7200194.1"
                     /translation="MNNAEFLQHKAMAERDYGVILPEAKAYLTDAVANSYSYAMDSQP
                     SLVTTSNSGIPWYFTNYVDPELIRILVTPMKAVEILGETKKGDWTTMTAQFPVVESTG
                     QVSSYGDYNNNGQVNANVNWVARESYLYQTITQWGELELDRYGEGRIAWAQQLNTASA
                     LTLNKFQNKSYFFGVAGLKNYGILNDPNLPASITPGATGASGGTTWATKDGQAVYDDI
                     QALYMQLVKQTKGYVERDSKMTLAMSPESEANLTKTNMYNVNVSDQLKKNFPNLRVVT
                     AVEYKTESGELVQLIADDLDGQDTGYCAFTEKMRAHPVVVDLSAYKQKKTGGTWGAII
                     RQPLAFASMLGV"
     gene            complement(7496..7975)
                     /locus_tag="M2A35_003964"
     CDS             complement(7496..7975)
                     /locus_tag="M2A35_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200195.1"
                     /translation="MSGFQSVINQQQAPGVEGDFASANPKASLLAGEGALVAGANGVV
                     VGRFAWVSNGVVNNTGTGAPAGFVHREGQASVTTWLGESSMTIQPGVQMTLMTAGDYW
                     VKTAGAATVGQKIFAKLSDGTITAGDAGASISDYVETKFVVGSAGEAGELIQMGTWS"
     gene            complement(7979..9205)
                     /locus_tag="M2A35_003965"
     CDS             complement(7979..9205)
                     /locus_tag="M2A35_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCB7200196.1"
                     /translation="MPLRKVKGDWQWGYHGLAFDKATVRSFDKDGRLHIEVTPISKAN
                     VCSYYGREIPNYRSLGLQPDKVYYLLRDPKELAKAASTFNNIPLLNEHIPVTASDPQK
                     MAVVGSTGTDAEFDGTYLKNSLVVWDADSIAGIETDEKKELSSAYRYVADMTPGVHEG
                     QPYDGVMRDIVGNHVALVTEGRAGSDVVVGDSIPTGMKSMSELTKKLMAVITPMLASD
                     EKPEEVEKKVQKVVEDEATQAERDNESEAERLKREEDELKEREERERKDRDRDRKEAE
                     DEDDDGKDKKTAEDEDDDENKAAMDAALIRKAEENVMGRIRQANEARECVRALVGDVS
                     LVAMDSAESIYRFALDSIGAEHKGVHPSALKSMVEFAISQKAVVRKPSHGMGMDSAAT
                     NSFAKAFPGATKLKRS"
     gene            complement(9340..10026)
                     /locus_tag="M2A35_003966"
     CDS             complement(9340..10026)
                     /locus_tag="M2A35_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCB7200197.1"
                     /translation="MDKSYRYWLEGAYKNNMAMDSSPANELRRRLSRLGKQWEHKFNE
                     LAKKLADRFVDKTLRNTDVSLHSALKAGGFTVKFTMNDELKNVMQAVVNENVNLIKSI
                     PEHYHTQVETIVMQSVSRGRDLGYLTDELVKRYGITRRRAETIARDQNNKATAVIQSE
                     RQKKLGITKGIWRHSHAGKQPRPSHVKADGKEFDLDKGLYLDGEWVLPGEAINCRCTW
                     SPVIPGIDRK"
     gene            complement(10079..11554)
                     /locus_tag="M2A35_003967"
     CDS             complement(10079..11554)
                     /locus_tag="M2A35_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCB7200198.1"
                     /translation="MSLANVLAIINEQERIKNEGEALRRIERYVPPPGVIPEHIGESA
                     LAMDSTPYSYLNSANITAYGYGGFPGYPYLSQLAQLPEYRKIAGTIAEEMTRKWIELK
                     HVGKDDGDDKADKIRQLDDALKRFRVREKFREAAEHDGYFGRGQIYIDVKTPSGNSAW
                     LVPDELDKKLYISPRKITKGSLNGFRVIEAMWTYPGVYNADNPLSPDFFNPSEWYVMG
                     RTVHASRMLTMISRQVPDILKAAYNFGGLSLSQMAEPYVQNWLRTRDSVSDLVHSFVV
                     HGLKTNMQNALSGIADPNLFMRAELFNKVRDNRGMFLIDKDGEEFFQFVTSLSGVDAL
                     QAQAQEQMASVSSIPLVKLLGITPNGLNASSDGEIRVFYDSIHAMQENLFRVPLKTVL
                     DVIQLNEFGEIDPDIDFEFLPLYELTEAEKAEIMKHQSEADKNYAEAGVFDLDAIRSM
                     RQSDKANPYHMMESEYDEEEYENESIEEGFEDPENPSSSQS"
     gene            complement(11614..13134)
                     /gene="terL"
                     /locus_tag="M2A35_003968"
     CDS             complement(11614..13134)
                     /gene="terL"
                     /locus_tag="M2A35_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCB7200199.1"
                     /translation="MAGAPISLNRNAQIYELHKELAIRSARKNLLDFTLYTNPQYETG
                     WFNELLCAELDHFLDEVKAGNMPRLMVFAPPRSGKSELCSRRFPAYVLGQHPSWNIIS
                     CSYSSDLSDRMSRDVKRIITSDKYADVFPDVKIPSGRSLAGGINKTELWEPVDAKGEL
                     HGGSYRSAGVNGGITGQGMNIGVIDDPAKDYKTASSPTYQEAVMDWYDTTFFTRVDPK
                     INGIVIILTRWHQNDLAGQLLKLAEEGGERWRVVSFPMEAEKEEIHELNGNVYRLRNP
                     GEILFPERMPREFVEKCKQRGSLVWNALYQQRPTAKGGGLIKSEWFGEYSVLPPMQWR
                     AVYGDTAQKTKEVNDFSVFEHWGLGTDGYIYLIDMIRGKWEADELQRRAVAFWEKCKT
                     LKNGPLRHMAIEDKSSGTGLIQNIRKKAICPIKAIQRDKDKYTRLMDVQGYIESGYIK
                     LPSSAPFINDFLVEMEAINPDFNTHDDQLDPMMDAIDEMKNGNGPLRISEDLLRLA"
     gene            complement(13088..13678)
                     /locus_tag="M2A35_003969"
     CDS             complement(13088..13678)
                     /locus_tag="M2A35_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCB7200200.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEKTARATGSENLS
                     KPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKPIKDWPKVW
                     RTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGKHVSVQAFKEQTSTEITGADG
                     GPVRYADMSEELLEEKLKELGNGRRSNQLESKRSDL"
     gene            complement(13810..14013)
                     /locus_tag="M2A35_003970"
     CDS             complement(13810..14013)
                     /locus_tag="M2A35_003970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200201.1"
                     /translation="MTPIDFIHKNVTTELIKLGYDQNAAMTGADMAVEHYRRCSQASR
                     KGRIFDDCLYIAKQWAGKQKGKK"
     gene            complement(14010..14606)
                     /locus_tag="M2A35_003971"
     CDS             complement(14010..14606)
                     /locus_tag="M2A35_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7200202.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPKVLGDAAAKFFATDTYINGTGGVVERDICNFPKREACLMAMSYS
                     YELQAQVYDHMTELEGGKDINLLDFSGLTDMAISEMQNRVAAAEKFSFEMHGQAGSAL
                     MTRRKKEKKAIKKAEQLVKDLIQFKLCDMGDFPDGKPA"
     gene            complement(14809..15246)
                     /locus_tag="M2A35_003972"
     CDS             complement(14809..15246)
                     /locus_tag="M2A35_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCB7200203.1"
                     /translation="MSRVTAIISALVICIIVCLSWSVNHYRDNAITYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(15243..15719)
                     /locus_tag="M2A35_003973"
     CDS             complement(15243..15719)
                     /locus_tag="M2A35_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCB7200204.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDVYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(15703..16026)
                     /locus_tag="M2A35_003974"
     CDS             complement(15703..16026)
                     /locus_tag="M2A35_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCB7200205.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(16609..17223)
                     /locus_tag="M2A35_003975"
     CDS             complement(16609..17223)
                     /locus_tag="M2A35_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200206.1"
                     /translation="MRLTPVFSMVNFINDAHFRRVWRHPKKTINSSQKAWVHYMLQVW
                     GKVNAGDCSPGGAINVIGRLMIRSQWSDDKAKQIESVVMRLYEEDGLRGDELYKKARE
                     LVIPQSSFSHIITLAKESDDAAFVERVMVKTFHRESPVRDVAIKRYCNRNCTQDIARM
                     ISYITGADIQYCRKRVVWCEKVLESEMFYAMKRELEKEIPLIAA"
     gene            complement(17223..17366)
                     /locus_tag="M2A35_003976"
     CDS             complement(17223..17366)
                     /locus_tag="M2A35_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200207.1"
                     /translation="MNKAFERYVRQRYGNRYDLTRDVDGFYCREIVKRMFEVWCHCKG
                     WEV"
     gene            complement(17363..17725)
                     /locus_tag="M2A35_003977"
     CDS             complement(17363..17725)
                     /locus_tag="M2A35_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7200208.1"
                     /translation="MNEYQFVLPYPPSLNTYWRRRGSQYYISDKGQKYRKDVQKIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(17722..17937)
                     /locus_tag="M2A35_003978"
     CDS             complement(17722..17937)
                     /locus_tag="M2A35_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCB7200209.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYREIDRRTHNLD
                     NKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(17937..18659)
                     /locus_tag="M2A35_003979"
     CDS             complement(17937..18659)
                     /locus_tag="M2A35_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCB7200210.1"
                     /translation="MNELINNNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     PMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVDFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(18734..19414)
                     /locus_tag="M2A35_003980"
     CDS             complement(18734..19414)
                     /locus_tag="M2A35_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCB7200211.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEILNIQVNFTRN
                     KERFVEGKHFFKASGEELKNLQLTLSKSQNPISPKARSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFSQCEKNTGKQEKKPNGLSAKETDILVWLWDYANRSQALFRELYPA
                     LKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLNNKE
                     LPPSLHRY"
BASE COUNT         4718 a         5014 c         4438 g         5510 t
ORIGIN      
        1 tgccattgct tcgccatccc atcgcaactt ttaactatct aacgccactt gaaaatgcct
       61 gatttactga tgatctgcga ttcaactcct taaatccttc ggttagtttt gaggattgat
      121 ccgttttggc aattaccttt acctctccta tgtgctggct gttgtaattg ttaacaacgc
      181 tctgggcata ggattggttt gcagatccat tagccaatct ttgctgggtt tggtagtacg
      241 cggcattgct atttttcgtt gggttaccat aatcaagacc ccgcttccaa tctggataac
      301 cttcatgcct agccatatag gcgctaagtt ttgccataac ctctggatct gagaggttta
      361 gcttatcatt ggcaccaacg cccatagact tagaaacctg gtcaatatac gcctttatgt
      421 tgttttctga ggatggtgcc cacttgctta ttatcccttg caatgtatca agcttatctc
      481 ttttgtagta tagagtcagt tgattcctgg cggcctccca gccctcttct gggtcgaagt
      541 attgagcaaa tccgtccttg tctcttcttc tgtttccagt agctttaaga tttaatgggt
      601 tattgtttct gtcaggcctg ttgacaccat ttttgttagg aggaatctct ttatttggat
      661 cgtcataatc catcccaatg agcctttcca gccaatgctg ctgaaatgcg aatggccgca
      721 tctcatcagg aacatcttta tactggtctt gtaccatctg cccaaaaata ccaacagccc
      781 cgattaatcc cagcttgctt cctaaccctt tgccgctaag aagagccatg gcagcaagaa
      841 tgcccaatgc gtttccagcg ccaccaacgg ctttaacaaa accatcaata cctgtactca
      901 ggttcctgaa gaacccaaca atgtcaccct tgttgttttt cagccactct aaaaactcct
      961 gttctgcttt aagcacttca gggccaaatg tagccattaa ttcttgttta acctgagcaa
     1021 attgcgagtc aagggcttta gtcgtagcaa gaatattacg ctgtgcttct tcctgctctt
     1081 tggtcatctg ccatcgtttt tcttcctcag ataccatctg cacagctttt ccgcttttaa
     1141 tatccccggc aagattaggg tcataaccaa aagcagaaag cacttgcatt aactgttttt
     1201 cgttatggtt cttcccatat ttctgaaatt ccgccagagc atcttctgat ttacctccaa
     1261 gcttactaat atcaatgcct gttctcgctc cagttgttag taaattctga gtctccggtg
     1321 taagtccgcc gaaaattgtt gggtcttcta tattggcaag cgccatcctg gcgctcatct
     1381 gtgccccaag gaacgcgccg ccgttctgac caattcggga gaatccatac tgagtaccaa
     1441 gaacgttact ggcgcttgtt ccaagcattc tccccatatt actggctctt acaattgact
     1501 ccgaagtact ttcaaatgca cgatgaatgc caatggctac ggcagtcaac acaccgccaa
     1561 cagccacaaa gcgtgtcgaa agccccaaaa aaccattaaa tgaagcagtc aaccctttca
     1621 ttgcaacagc agctttgtcg gtctccttag ccgttttgga catcgaggag gttgttgacc
     1681 tttctatcgc agatgaagat ctctctattt cgtttttaag gtcttgtgct tcctgcttta
     1741 ctttccgttt tccattcagg aattcatcgg ccttaattgt gaccttgtag gccaactcat
     1801 tgataatcat cgctgctcct gatgtttatt ccagacccgc ttgttaaatg attccaccga
     1861 gataatttcc agaaggttat acatatcacg aacagataac cgttcctgca aatcggtata
     1921 agtggctttt cctgaacaga taatggcgtt cattgatggt gtgacgttta cggggctaac
     1981 gagttttgcc ggaagagtct cctcctccat gaacgggtac ttcactctcc ggcgatcgtt
     2041 aaaaaatcaa aattgacttt aaatacctta tccattacct gacgaatggt tgatacctct
     2101 tcaaagtcga caggcttcac ggttcgcacc tgtctgctgc cttcgtgtgt aatcacaatt
     2161 tgcacagttg acatcaggcg atcacgaagt tttctggcga cttccggcga tgcagcagaa
     2221 ataacattca gaccaagggt ggcaaggcca gcacacccca tggcaatcac atcagcagga
     2281 ataccagaga atcccgattc ccccatggag cggaataaat cctgtgccag ttcgtcggca
     2341 tcccatgccg acatctctgt gataatgaac tctttcccat tgtcgcgatt atcgtcttcc
     2401 acgataaagg ggatttcttt acgtgccatc agatagtgct ccgtgttaca gactcaaagt
     2461 gaaatactgc cgggcgtggt tgcagtacgc gacgacccgg aggggttgga gtccatgtat
     2521 aaagaacccc gttcacaaaa ttccacttcg cgccgagagc cggaactgta agcaccgcat
     2581 tacactgaaa cgctgaaatt gcggttctct ccgctgcata ccagtcatca atcagcgagc
     2641 cagcattgga tgtgggcatc aggttgatgg tgaactctgt cgggttaaag ataaaaccag
     2701 catggtattt accgtccgct gacatcatgt cttctttgtt ctgtagtgcg ccagtttcaa
     2761 acatgttatc agctgcgtag tcgtcaacat caaaaccgcc agggtagtaa gcgggtacga
     2821 cgattcgcag cttactgttt gccgaggtaa tatcgatagg catgatttat tcccttataa
     2881 aatcgcggtt gaggacattg tgatggactg gattaactga ccatcgacat aatagaaaat
     2941 gacacctttc aggtcgcgct caatgcgtgc agagcctgac tgtgtcggga tgtacaggaa
     3001 ccagccctgt gaatacagcg tcgatgagat atcttttcca acagtgttat tcacaattct
     3061 ggtttgcgca ttatcaagtt tcacgccacg ctgaatagcc ccgaaattga gagcctgttc
     3121 agcaacatca ataacagcag cagaaactgc gccgtatcct gtctcattga acgggtaaga
     3181 ctggttattg gtgaacaggt tggcaaaagc actaaccaga ttggcattaa tccatacctg
     3241 attaataaaa ctgtccagcc atacaaactt gccagtaatg gcaccatcag atgcgtactg
     3301 cgccattgtt ttgttcaggc tgtatgagcc gtagaagttg taaccgtttg acttcagtgc
     3361 ctgagcggtc gccagatcgc ttacgttggg tgctaaccct gaaaatccac ggaacttgaa
     3421 cgagatacgc ccattggtgc gtgcaaaatc cacggatgca gcatatgcca gtgctgtaac
     3481 gctatacagg tatgttccgt acaccggaaa aatgttctcg tagccatttg ccacaaccac
     3541 tttctgcaca aagcaattgg cattattggc taccgttcct gctgaagtgg tgtcgtgaac
     3601 aacatatcca aaccggtttt tactgctgct tgcccatgcg cacaattctg ttttctggtc
     3661 atcggttagt tcgaccagcg agttaaacag gatccagttc tggttgacgt tgatgatgtt
     3721 gttcatcgtg tctgtcagcg ttaccgcatc agaacccggt gatacaatcg ctgcggaatc
     3781 ctgcgtcagt aacaacccgg tagccagagc gccagcagaa gcataagaca cttcactctc
     3841 tgcgccagca gtggcagagc gaatgatgaa tcggttagcg attggcaacc attcaaccac
     3901 caccttgctt gcaccaattc caacctgcaa cttggacgca atgtcactaa aacttgtggc
     3961 tgtggacaaa tcaattgatg tgctggtagt cgactcaccg tcaatggaaa gggtgattgt
     4021 tcctgctgga atcgctttca gtgttgccag ggtaacacct ttcaggttgc cggacagaag
     4081 atacccagcc actggtgaag taacaatacg atacatcagc agttcaccag gaataacgga
     4141 tgagttttcg tatccgttaa aatactgttg tgcggcgagg aattctttcg attcactccc
     4201 catcaaagct gatacatcag atgaggagta ataagattgt accgcgccaa ccgggataag
     4261 ctcgttatcg gtcaacatca ggccgttagc atcaaccgca gaaccggcag gcgtaacgac
     4321 attgggcgtg atattaaaat ccgttgataa tggaatggtc atatatctac ctgttgtaaa
     4381 gtgatttcgg ccttgtcgaa gtaatcctgt gggaacgaca cggtaatgtg agcctgtaga
     4441 gaaagagtca gcgtccatct ttcctgccac tggctttccg catctatcat gggagcctga
     4501 atggcaggtg atgagtaaag cggagcaagc cgggcatcga tggctttgat gacgtcgtag
     4561 ccatatccgc ttgtgaatgt tgtttccagt gcaatagccc gatcccctgc accctgacca
     4621 tagatatcca cctgaatatc agcctggcga acttcggtgt atctcatggc gcttgtcgat
     4681 gggtcgccag tgtcttggct tacatcgcgc gtcgtggata gcctggggaa tcgcaaaggg
     4741 gtcaggatgc agaactggcc ttttggcatt ggtacgcggt tggcctgagc ctgctggcat
     4801 ataccggcga tcggctcgat atatccagcc aaatcatcaa taatttcgtc tagcgtataa
     4861 tcattcatgt gctcacctgc aatacagcaa tcagacgaca ccagttcggc cacaactcta
     4921 ccggttcgac gacaagccat tgctcaccgt taatcacgaa gatatcgccg ccctgctcca
     4981 gttctcgctg aacactgaag taattaccat tgacgtaaat cacttttgcc agcccctgaa
     5041 tattcagtcc atcgacgtgt tgcatgtcgc cgcgactgat tggctgtagt tgaatggtaa
     5101 cgtgctggtc agggagataa gacggaatcg gcttgcgacc ggggccgata gtttcacctg
     5161 catacttttt caggattgcc gggatgttgg ggttaatgct tgtgatcgcg ttattggcta
     5221 tctgtctgag attcaatttc gctcacctca tagctgacgc tgtccagcat gtttttggta
     5281 tcaatcagcg gattatcaaa tccctttctt gcgatggtaa ctggtgaaag tggcggagaa
     5341 tcaagggagc gaatcgactg ctgtatatca gcggcgataa tagttcccag cattgaaaat
     5401 acttcttcaa tgtcatcgcc acgctcaatt agcaaagcag cctgctcttt ccactcttct
     5461 tcatgctctg cgattgcatt tctaaaaaaa ggtcgaggag gctgattatg agccggattc
     5521 ccaaactcgt tggtagcggc aaccatgggg accgggatta caccgccgta agtagccccc
     5581 tcaaggaagc caacctttag ctgaatgttg gaaaattgct tttcaacctc atccagcgca
     5641 tccatcacct tatccatcag tacctccggt aatacccata cgggtaattt gatggagaat
     5701 gcccgcttat atactggaag gtgcgaaacg gcgccgtagc attccagtaa tcagccccgt
     5761 actttgtttg catgtaccat gctgaatttg ctgttacccc tggcatatcc gcatgaacgc
     5821 taactgaacc ctctgaagca ctgtcgattc ttccaaccaa tccggatgga gactgcccat
     5881 tcgctcctga atacaaacag gcgatatggg caaccagcat attcagcagc attgagcgta
     5941 cagccaaatc cgacacacga cttgagtctg tgttatcgag ataaatggtt gcttgcgtga
     6001 aatactgctg aagaagtgcg tcgtcgacag atgagaattc aggatagcgt agcttaaatg
     6061 cagaggggtc aaatattacg acgcccattt gctgctcccg ctatttttcg tcagcctttt
     6121 ttacgcccgg agccggattt tccgggtcaa gaccttcgag gccagttttt gattctttca
     6181 gatctttacc ctgcgcattc agactgcgaa tatctttctg aatgaagatt gcattgctct
     6241 gaatatacgc ggcatcctta taggtctcga tgaacttatc catgaagtct ttctcgacca
     6301 tggtaactcc gaaagccccc tcaggaattg caccatcaag accacggagg gcagttgttg
     6361 ccgccccgtt aagaattacc gtttttccat ccagagttac ctgaaggcca tttggcaatt
     6421 tgcagccaac gcttaccatt tctgccatga attaaactcc caacatgctt gcaaatgcca
     6481 gcggctggcg aatgattgcg ccccaggtgc caccggtttt cttttgtttg tacgcggaca
     6541 ggtcaacaac aaccggatgc gcacgcattt tttcggtaaa tgcgcagtag ccggtgtcct
     6601 gaccatccaa atcgtcggcg atcaactgaa ccagttcgcc ggattcggtt ttgtactcga
     6661 cagcagtaac aacgcgcagg ttcgggaagt ttttcttcag ctgatcagac acgttgacgt
     6721 tgtacatgtt ggtcttggtc agatttgctt ctgactctgg actcatcgcc agcgtcatct
     6781 tgctgtctcg ctcaacgtac cccttggtct gcttcactaa ctgcatgtaa agcgcctgga
     6841 tatcgtcata caccgcctga ccgtctttgg ttgcccaggt ggttccgccg ctcgcaccag
     6901 ttgcacccgg ggtgattgat gccggaaggt tagggtcatt cagaatgccg tagttcttca
     6961 ggccagcaac gccgaagaag taggacttgt tctgaaactt attcagggtc agcgcagaag
     7021 cagtattcag ttgttgagcc catgcaatgc ggccttcacc gtaacggtca agttccagct
     7081 caccccactg agtaatggtc tggtacagat aggactcacg agcaacccag ttgacgttag
     7141 catttacctg cccattattg ttgtagtcac cataactgga aacctgaccc gttgattcca
     7201 caaccgggaa ttgtgcggtc atggtggtcc agtctccctt cttcgtttcc cccagaattt
     7261 ccaccgcttt catcggagtg acaaggatgc gaatcagttc cgggtcaacg taattagtga
     7321 agtaccatgg aatgccagag ttgctggttg ttaccagtga aggctgagag tccattgcat
     7381 aggaatagct attagcaaca gcatccgtca gataggcttt ggcttccgga aggattacac
     7441 cgtaatctcg ctcagccata gctttatgtt gtaaaaattc tgcgttattc atgtattagc
     7501 tccaggtgcc catctgaatc aattcgccag cctcgccagc actgccaaca acaaattttg
     7561 tttcaacgta atccgagatt gacgccccgg catcgccagc ggtaatggtt ccatctgaca
     7621 gtttggcgaa aatcttctgc cctacggttg ccgcaccggc agttttcacc cagtagtcgc
     7681 cagcagtcat caatgtcatc tgaactccag gctgaatagt catagatgat tcaccaagcc
     7741 atgtggtgac agacgcctgc ccttcccgat gaacgaaacc ggcaggagca ccggttccag
     7801 tgttattgac aacaccattg cttacccatg caaagcgacc aacaacaacc ccatttgcac
     7861 cggcaacaag agcgccctca cctgcaagaa gacttgcttt agggttagcc gaagcgaaat
     7921 ccccttcaac tccaggcgcc tgttgctgat taattacact ttgaaagcca ctcatttctt
     7981 agctccgttt cagtttagtt gcgccaggaa atgccttggc gaaagaattg gttgcagcag
     8041 aatccatccc cataccatga gatggtttgc gaactaccgc tttctggctg atagcaaatt
     8101 caaccatcga tttaagtgct gaaggatgaa cgcctttatg ttcggcaccg attgaatcaa
     8161 gagcaaagcg ataaatagac tctgcggaat ccatagcgac cagactcaca tcaccaacca
     8221 aagcacgaac acattctcga gcctcattgg cttgacggat acgccccatc acattctctt
     8281 cagcttttcg aatcaatgcg gcatccatag ctgctttatt ctcatcatcg tcttcatcct
     8341 cggcagtctt tttatccttg ccgtcatcat cttcgtcttc cgcttctttg cggtcacgat
     8401 cgcgatcctt tcgctcacgt tcttcacgct cttttagttc atcctcttca cgtttcagac
     8461 gctctgcttc tgactcatta tctctctcag cctgagtggc ttcgtcttca acaacctttt
     8521 gcactttctt ttccacttct tctggcttct catcactggc cagcatcggc gtgataactg
     8581 ccatcaactt tttagtaagt tctgacattg atttcattcc tgttggtatt gaatccccga
     8641 caacaacgtc ggatccggct cttccctctg ttacgagagc aacgtggttc ccgacgatat
     8701 cgcgcattac accatcgtat ggctggcctt catgcacgcc gggggtcatg tcagctacat
     8761 atctgtaggc cgacgaaagt tctttcttct cgtctgtctc gattccagcg atagaatctg
     8821 cgtcccatac gacaagcgag ttcttgagat aagtcccgtc aaactcagca tcagtaccag
     8881 tagaaccaac cacagccatc ttctgagggt ctgaagcagt aaccggaatg tgttcgttaa
     8941 ggagcgggat gttgttgaat gtagatgctg ctttggctaa ctctttcggg tcgcgaagca
     9001 ggtagtaaac tttgtcaggc tgtaaaccta gcgatctgta attagggatt tcgcgtccat
     9061 aataagagca aacgttagcc ttactgattg gcgtcacttc gatatgcaaa cgcccatcct
     9121 tatcaaagga gcgcaccgtt gccttgtcga atgccaatcc gtgatatccc cattgccagt
     9181 cgcctttaac tttgcgtaat ggcatgcttt tacctttcgg taggcaataa aaaagccgca
     9241 gtagcggcca ttatttcctc ttgggcatag ctattttcag tagtgcaatc attaagcaac
     9301 caaaaatcaa cactaagctc agagaccata aaacctcaat cattttctat ctatcccagg
     9361 gataacagga gaccacgtac aacggcagtt gatagcctct ccaggcagca cccattcacc
     9421 atcgagatac agccctttat cgaggtcgaa ctctttaccg tcagctttca catgggacgg
     9481 ccttggctgc ttcccggcgt gtgagtgacg ccagatacct ttggtgatac ctaacttctt
     9541 ctgtcgctca gactgaatta ccgctgtagc tttgttgttc tgatcgcgtg caatcgtctc
     9601 agcgcgtctg cgtgtaatgc catatcgctt aaccagttca tcagtgagat agccaaggtc
     9661 acgaccacgg ctgacagact gcatgactat cgtctcaacc tgtgtgtgat agtgttcagg
     9721 tatggattta atcaggttga cgttctcgtt aacgacagcc tgcatcacgt tcttaagctc
     9781 gtcattcatt gtgaacttaa cggtaaatcc gccagccttt aacgctgagt gtaacgaaac
     9841 gtcggtgttg cgcagtgtct tatcaacgaa gcgatcagcc agtttcttag ccagttcatt
     9901 gaatttatgc tcccactgct tgcctagtct ggataatctg cgccttaact cattcgcagg
     9961 actggaatcc atcgccatgt tgtttttgta tgcgccctca agccagtatc ggtatgactt
    10021 atccatctcc ctaaccagtt taagaagctc agagcgatac cactcatgaa ctccggcgtt
    10081 agctctgact ggacgaaggg ttttccgggt cttcgaatcc ttcttcgatg gactcgtttt
    10141 cgtactcttc ttcgtcatat tcagattcca tcatgtggta tgggttggcc ttatccgact
    10201 ggcgcatgct tcttattgca tcaagatcga acacgccagc ttcggcatag ttcttgtcag
    10261 cctcagactg atgtttcatg atctcggctt tctctgcttc tgtaagctca tacagaggca
    10321 ggaactcaaa atcaatgtca gggtcaatct cgccaaattc gtttaactgg atcacatcca
    10381 gtactgtttt cagtggtact ctgaacagat tctcctgcat ggcgtgtatt gagtcataga
    10441 agacgcggat ttcgccatca gatgaagcgt ttagcccgtt aggagtgatg ccaaggagct
    10501 taaccagagg aatgcttgat accgatgcca tttgttcctg cgcttgcgcc tgaagagcat
    10561 caactccgga cagacttgtc acaaactgga agaattcttc tccatcctta tcaataagga
    10621 acattccgcg attgtcgcga accttattga atagctcggc acgcataaac aggtttgggt
    10681 ctgcaattcc agacaaggca ttctgcatgt tcgtcttcag cccgtggacc acaaacgaat
    10741 gaaccagatc gctaacgctg tcacgtgttc tcagccagtt ttgcacatat ggctctgcca
    10801 tctgactcag cgacaagccg ccgaagttgt acgccgcttt gagaatgtct ggcacctggc
    10861 gagaaatcat tgtcaacata cggctggcgt gaacagtacg ccccatgaca taccattcag
    10921 atggattgaa gaaatcagga ctgagagggt tatcagcgtt atacacacca gggtatgtcc
    10981 acatggcctc gataacgcgg aaaccattca ggcttccttt ggtaatcttg cgcgggctga
    11041 tatagagctt cttatccagt tcgtcgggaa ccagccaggc tgagttaccg cttggcgtct
    11101 tcacgtcgat ataaatctgt ccacgcccga agtaaccgtc atgttccgcc gcctcgcgaa
    11161 acttctctcg cactctgaaa cgctttaatg cgtcgtcaag ctggcgaatc ttatcggcct
    11221 tgtcgtcacc atcgtcttta cctacatgct tcagctcaat ccattttcgc gtcatctctt
    11281 ctgctatcgt gccagcaatc ttgcgatact ccggcaactg agcaagttga gacagatatg
    11341 ggtagccagg gaatccaccg tagccatatg cggtgatgtt tgctgagtta aggtaactgt
    11401 acggcgtgga gtccatcgcc agagctgact ccccaatatg ttcaggaata acgcctggcg
    11461 ggggtacgta tctctcaatt ctacgcaatg cctcgccttc atttttaatt cgctcctgtt
    11521 cgttaataat cgcaagaaca ttcgccagag acattggctg tttcgcctct tctttctgag
    11581 gctcagattt tttctttctc aaaaaattaa acactatgcg agcctcaaaa ggtcttcaga
    11641 tatacgtaac ggaccattgc catttttcat ctcatcgatg gcatccatca tcggatcaag
    11701 ctggtcgtcg tgcgtattga aatcaggatt aatggcttcc atctcaacga ggaagtcgtt
    11761 gatgaatggt gcgctgctgg gcaacttgat gtaaccggac tcgatataac cctgtacgtc
    11821 catcagtcgc gtgtacttat ccttatcgcg ctgaattgct ttgatggggc atatcgcttt
    11881 tttacggatg ttctgtatca ggcctgtgcc agatgacttg tcctctatcg ccatgtggcg
    11941 aagcggccca ttcttcagag ttttgcactt ttcccagaat gcaacagcac ggcgttgtaa
    12001 ttcatcggcc tcccatttac cgcgaatcat atcgatcaga tagatgtacc cgtcagtgcc
    12061 aagcccccag tgctcgaata ctgagaagtc gttgacctct tttgttttct gtgcggtgtc
    12121 tccatagacg gcacgccatt gcatgggagg gagtaccgaa tattcaccaa accattcaga
    12181 cttaataagc cccccgcctt tggcggttgg tcgttgctga taaagggcat tccatacaag
    12241 agaaccgcgc tgtttgcact tctctacgaa ctctctcggc atgcgctcag gaaacagaat
    12301 ttcaccggga ttgcgcagtc gatacacatt accgttgagt tcatgaatct cttccttttc
    12361 agcctccatt gggaagctaa ctacgcgcca gcgttccccg ccctcttcag ctagttttaa
    12421 taactgtccg gcgagatcgt tttgatgcca gcgtgtcagg atgatgacaa ttccgtttat
    12481 cttcgggtca acacgagtga agaatgtcgt gtcataccag tccatcaccg cttcctggta
    12541 agttggagac gatgcggttt tgtaatcttt cgcggggtca tcaataaccc caatgttcat
    12601 accctgcccg gtgatgccac cgttaacacc agcagaacga tatgagccgc cgtgtagttc
    12661 gcctttggca tcaactggtt cccatagctc ggttttgttg atgcctccag ccaaactgcg
    12721 tcctgagggt attttcacgt cagggaaaac atcagcgtac ttatcagagg tgataatgcg
    12781 tttaacgtca cggctcattc ggtctgacag gtcagaagaa tatgagcacg agataatgtt
    12841 ccatgatgga tgctgtccta acacatacgc tgggaagcgg cgagagcaca actcactctt
    12901 tcctgagcgc ggaggggcaa acaccattag cctgggcatg tttccggctt taacttcatc
    12961 cagaaagtga tccagctcag cacaaagcag ctcgttaaac caacctgttt cgtattgcgg
    13021 attagtgtac aaagtgaaat cgagaaggtt ttttcttgct gagcggatag ccagctcttt
    13081 gtgcaactca taaatctgag cgtttcgatt caagctgatt ggagcgcctg ccattgccaa
    13141 gctccttcag tttctcttca agtaactctt ctgacatatc ggcataacga acaggcccgc
    13201 catcagcgcc agttatttct gtagatgttt gctctttaaa cgcctgaaca gaaacatgct
    13261 taccaagaag ttcgagattt ttgaccttat caggccattt gattttcttc aggagagcag
    13321 cgctatcagc agatgccatc tcgacgacat ccatacccga tagagtcgtt ctccatactt
    13381 tgggccagtc tttaatcggc ttcagttcgc cattggcaag cagaatgtca agcacgtcca
    13441 tctgatcgat ttcagtcagc cgtcgcaata cataggcagc gtctatacct acctcttcat
    13501 tgcgttcagc ctttagctca gcaatgcgat ctgctatgtc aggttttgac aggttctcgc
    13561 taccagtggc gcgagcagtt ttctcgctgt aacccgccct gatagctgcc tgagtggcgt
    13621 ttaaatcttt caggtactca cgggcaaaca gctcttgttt gtcggtgagc tttgccatta
    13681 tttgtgctcc gtttatccgt taaaagggat atcagttaag ttatcccgtg tagggtataa
    13741 gccattatca aagccaccag tagatgggct ttgtaatgga gagccgttgt gaaagtggct
    13801 ctcagactgt cattttttcc ctttctgttt acctgcccac tgttttgcaa tgtacaggca
    13861 gtcatcgaaa attcggcctt ttctgctggc ttgtgaacaa cggcggtaat gttccaccgc
    13921 catgtcagcg ccagtcatag ccgcgttttg gtcgtatcca agctttatta actcggttgt
    13981 tacattttta tgtatgaaat caatcggagt catgctggtt taccatcagg gaaatccccc
    14041 atgtcacata gtttgaactg aatcagatcc ttcacaagct gctcagcctt tttaatggcc
    14101 ttcttctctt tcttccgacg agtcatgaga gcgctacctg cttgaccgtg catttcaaac
    14161 gagaacttct cagcagccgc gacacggttt tgcatttcgc tgattgccat atcggtcagg
    14221 ccagagaaat cgagaaggtt aatgtccttc cccccctcaa gctcagtcat gtggtcatac
    14281 acctgcgcct gaagctcata gctgtagctc attgccatga ggcaagcttc acgcttggga
    14341 aaattgcaaa tatccctctc cacaacacca cctgttccgt tgatataggt atcagttgca
    14401 aaaaattttg cagctgcatc cccgaggacc ttgggtactt tcttcatgaa actgcggtgt
    14461 tcgagtttgc gatatttctt acaggggaac gcaagcccct ccgcctccgc tttggatttc
    14521 cgatcagcat taatgtaatc caccatctca agactgctca tggtcgggta ttcgccagaa
    14581 ggaagaacgg ttaatgattt gttcatgtcg gtatttcctt tagaaagttg agcctgttcg
    14641 cacagaaaag ccgtccccga gaggtcctca cctatacggc agttctcagg ctcagctttc
    14701 tgaaagactc gggattgtta cgcgctgcga tacgctgtga aattcagatg taaaaaaagc
    14761 cccgcatcgc gaggctcatt aaattgactt tgtgatttgc aaaaaaattt atttcaggca
    14821 ctgagtcctg atgtactcct gcaggtagtt aacctgcgcg gttatcctgt cgattccgct
    14881 tcggagacgg taataattga gttcagcatc tgctgtaagt cttgggcttt ctccatcgcc
    14941 catgccgctg gctccggtcg ttgactttgc acaggtggcg gcgacttgca ggcgcttacg
    15001 cccagcagaa acatcagcac ggagactttc gatagtcgcg ttagcatcag caagctcctt
    15061 tgtgtatctg gcgtcgagtt ctgctacatc acgttgacgc ttctgcatgt cagcgataat
    15121 ggatgtggct ttatcgcgct gctctttgta ggtaatggcg ttatcacggt aatgattaac
    15181 agaccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg cggttactct
    15241 gctcatacct caatctctct gaccgttccg ccagcttctt tgaattttgc aatcaggctg
    15301 tcagccttat gctcgaactg accataacca gcgcccggta gtgaagccca gatattgctg
    15361 caacggtcta tagcctgacg aatatcaccg cgatcaatca ttggtaaagc gccacgctct
    15421 ttaatctgct gcaatgccac agcgtcctgg cttttcggag agaaatcttt caggccaagc
    15481 tgcttacgat agacatccca ccaacgggaa agaagctggt agcgtccggc ggctgttgat
    15541 ttgagcttgg ggtttagcgt gacaagtttg cgagggtgat cggagtaatc agtgaatagc
    15601 tctccgccta caatgacgtc ataaccatga tttctggttt tctgacgtcc gttatcagtt
    15661 ccctctgacc acgccagcat atcgaggaac gccttacgtt gattattgat ttccaccatc
    15721 ttctactccg gcttttttag cagcgaagcg tttgataagc gaaccaatcg agtcagtgcc
    15781 gatgtagccg atgaacacgc tcgttatata agcgagattg ctacttagtc ctgcgaagtc
    15841 gagaaggtca cgaatgaacc aggcgataat ggcgcacatc gttgcgtcga ttactgtttt
    15901 tgtaaacgcg ccgccattat atctgccgcg aaggtacgcc attgcaaacg caaggattgc
    15961 cccgatgcct tgttcctttg ccgcgagaat ggcggctaac aggtcatgtt tttctggcat
    16021 cttcatgtct tacccccaat aaggggattt gctctattta attaggaata aggtcgatta
    16081 ctgatagaac aaatccaggc tactgtgttt agtaatcaga tttgttcgtg accgatatgc
    16141 acgggcaaaa cggcaggagg ttgttagcac aacctcttgc cacccgcttt cacgaaggtc
    16201 atgtgcagaa ggccgcagcg taactatcac taatgaattc aggatagcca gtggctacgg
    16261 ctcagtttgg gttgtgctgt tgctgggcgg tgatgacgcc tgtacgcatt tggtgatcag
    16321 gttctgcttc cggtattcgc ttaattcagc acaacggaaa gagcactcgg tgcatttaag
    16381 ccaagcccca taatggagaa tgctcttgcc tgttgtgaaa acaaaaaagc cccgacgttt
    16441 ctgccgaggc tcttacctaa tctagccagt atgtcgaccc taaatattat cgacgaccgg
    16501 ggaaatcagg atttcaattt cgctgctcag ttcgcttttg ctccgagcat atacgaaaaa
    16561 taccactttc atttctcgga agcaacgcat ttacaaaatt atttcagttc aggccgctat
    16621 taatggaatt tctttctcca attctcgttt cattgcataa aacatttctg actccagcac
    16681 tttctcacac cacaccactc gcttacgaca atactgaata tcagcacctg ttatataaga
    16741 aatcatcctg gctatatctt gcgtgcaatt gcgattgcaa tatcgcttaa tagctacatc
    16801 gcggacaggg ctttcacggt gaaacgtctt gaccatcaca cgttcaacga aagccgcatc
    16861 atctgattct ttggcgagag tgatgatgtg gctaaacgat gattgaggaa tgaccagttc
    16921 gcgagctttc ttgtatagct catctccgcg cagcccatct tcttcgtaca ggcgcatgac
    16981 gacagactct atctgcttag ccttgtcatc gctccactga ctgcgaatca tcagacgacc
    17041 gataacgtta attgcaccac ctggagagca atctccggcg ttaaccttgc cccatacctg
    17101 taacatgtaa tgaacccatg ctttctggct agagttgatg gttttcttcg gatgtctcca
    17161 tacgcggcga aagtgcgcgt cattaatgaa gttaaccatg ctgaatactg gagttaaccg
    17221 catcagacct cccatccttt gcaatgacac cacacttcaa acattcgttt cacaatttca
    17281 cgacagtaga aaccgtcaac atctcgcgtc aggtcatagc gattgccgta acgctgacgc
    17341 acatatcgtt cgaatgcttt gttcatacgt tctccagttc ggtgattttt attccaagcc
    17401 ttccgcctgg tactttcacg ccacgaatta cgcgaatgtc atcgaattgc tcgtcgtctt
    17461 ccgcaaatcc ggcgtggata agggagtcga gtaaaccctt caggatgtta tcgaggtcgc
    17521 ggcggcggga gtctggaacg tctgcgatga ctttgatgcg gagtcgtgat ttggtgaaaa
    17581 tgtctaactt gagttggcgg atgattttct gaacgtcttt tcggtatttc tggcctttat
    17641 cgctgatgta gtattggctt ccccgtcttc gccagtaggt gttcagcgac ggcgggtatg
    17701 gaagcacaaa ctgatattcg ttcatggttt aatcttcccc tccttcagca gtatcgcctg
    17761 cgtcctgatc acgccttcga ggtggtaaag tctggcgtct ttgttgtcga gattatgggt
    17821 gcgtcggtcg atttcacggt aatgtgccag aactgtagtt tcaggattgc cattgcatac
    17881 gccgtaaata cgtacctgac attctctgcc gcgcgcttct ttgcgtaggt tagccattaa
    17941 gcagcctccc ctgttacttt cagcattccg ttatcgagca gctttctggt cagccactgt
    18001 tgaccacgcc cggttatttt tgtggtgaac gatatctgta ttccgtgatt tgtgttgacc
    18061 gctgtttctt tcactgtgaa atagccgcga tccatatatt cctgcattgg cacatttcgc
    18121 cgagaacctg aagcaataag gattttgtga tcgcgcatcc acgcaaacag tttgtttgga
    18181 ccaataccaa caacctttgc aaagtttcca atcaaaattc cgctggcctc gccaacgcga
    18241 tcggcaaaat caactttagg tgctgcgaga gcaagctgtt tctccagttc agccttctgg
    18301 tcttcaaggt cagccgcgag gcgcaatgct tcagagaagg tttgtgggat tttcgcggtt
    18361 gccccttcga gttctcgcca gcggtcaaca aggcgagcgg tgaactctgg cgacaactgg
    18421 gcgacgacaa tgatactatc gcgcttacct tgttcgcctt cgaatatata cacgcttgtg
    18481 aatttgttag ggctaattgt ttgtttattt tcaacttttt gcattggagg aagttgaatc
    18541 accccacgct ttgccagacg ttctattgat attctgacat taccgtgttg gcttcccacc
    18601 aactcagcga tttcaatgct tgtcattttg atggcattgt tatttatcag ctcattcatt
    18661 gtcatgtcct ctcacattga aaattcagca ataaaaaacc cagccgaagc tgggtttgtt
    18721 aagttgtcaa ttgtcagtag cgatgcagtg aaggcggcaa ctctttgtta ttaagccttt
    18781 cccatgccag aaggttcgtc ggcccgtcag gctcataaat atctatatcc cgcgtgtgat
    18841 taattaaaac gcccctcgcc ctcccgatga tatacgagaa ctcatagccg tagtcgtggc
    18901 atatgccgga atagccagac tgaatcagtt ttaatgcggg atacaactca cggaacaatg
    18961 cctgtgagcg gttggcataa tcccacagcc atacaaggat gtctgtttct tttgcggaaa
    19021 gcccgttggg cttcttctct tgtttgccag tatttttctc gcactggctg aaatagcagt
    19081 cttccagttt ttcgaacaca tcccacgcct gatcggtttc gagcattttt gcatgacggg
    19141 ctgctccgcg ttctgtccag aggataaggg agcgggcttt gggagatatg gggttttgtg
    19201 acttacttaa agtaagttgc aaatttttta attcctcacc agatgcttta aaaaagtgct
    19261 ttccttcgac aaaacgctct ttgtttctgg tgaagttaac ctggatgttc agaatttctg
    19321 tgccataaag ctgcgccaaa agttcggtgg taataacagg aatctggtta tgggtgatcg
    19381 gggagagagt ttcaacagag atttgagttg tcataatgac gccctcgagt ggtttctaaa
    19441 ctatcaccac cgtcaggttc ctaatcatcg ggtggtgaga cgtacagggt taggaactac
    19501 cgggaaacca accggcgagc ttttcagctc ccccatacgc cccaccataa ttcagatgtg
    19561 cgcgtgcata cgacaataaa aaacacgctc gcggcgtgtg tctgtcgcgg tctctatccg
    19621 gggttcctaa tcccgacgcc agattttgct ggcgcgtgaa gaatatagcc ccaaataaat
//
LOCUS       DAHBHC010000088        19626 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHBHC010000088 DAHBHC010000000
VERSION     DAHBHC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.088"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="M2A35_003981"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="M2A35_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCB7200212.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQA"
     gene            complement(2101..2832)
                     /locus_tag="M2A35_003982"
     CDS             complement(2101..2832)
                     /locus_tag="M2A35_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HCB7200213.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLAVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(2864..3367)
                     /locus_tag="M2A35_003983"
     CDS             complement(2864..3367)
                     /locus_tag="M2A35_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HCB7200214.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            complement(3382..6204)
                     /gene="traG"
                     /locus_tag="M2A35_003984"
     CDS             complement(3382..6204)
                     /gene="traG"
                     /locus_tag="M2A35_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HCB7200215.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTRGSAGMY
                     GDAHAEWGVKPKILGVGGGVGVRGGGRASIDWSDEDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQNHHKTEALSQNNKYNEEKSG
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            complement(6201..7574)
                     /locus_tag="M2A35_003985"
     CDS             complement(6201..7574)
                     /locus_tag="M2A35_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCB7200216.1"
                     /translation="MLRIKPLFILCAALLAVTPAASADVNSDMNQFFNKLGFASNTTQ
                     SGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTRNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TANIYHCNDSDKCLNVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(7561..7986)
                     /gene="trbF"
                     /locus_tag="M2A35_003986"
     CDS             complement(7561..7986)
                     /gene="trbF"
                     /locus_tag="M2A35_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HCB7200217.1"
                     /translation="MNYQKYQRELIMKENKNNPELKIRSTERDYKYISRITDRYAVLS
                     LVFLTAGIVLWIVMNIIFDACIDSWKADPELNNVRYMWNILMYAIPCTLWALASGFFV
                     AGYLLPLCALPVRNIRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(7940..8281)
                     /gene="trbJ"
                     /locus_tag="M2A35_003987"
     CDS             complement(7940..8281)
                     /gene="trbJ"
                     /locus_tag="M2A35_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCB7200218.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWIFVSEIYYSCMTSLNKLS
                     EISEGTYNERK"
     gene            complement(8211..8756)
                     /gene="trbB"
                     /locus_tag="M2A35_003988"
     CDS             complement(8211..8756)
                     /gene="trbB"
                     /locus_tag="M2A35_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCB7200219.1"
                     /translation="MSLTKLLPVILLMMPTGVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQINLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(8743..9024)
                     /gene="traQ"
                     /locus_tag="M2A35_003989"
     CDS             complement(8743..9024)
                     /gene="traQ"
                     /locus_tag="M2A35_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCB7200220.1"
                     /translation="MHKYKFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLAE
                     LIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            complement(9140..9883)
                     /gene="traF"
                     /locus_tag="M2A35_003990"
     CDS             complement(9140..9883)
                     /gene="traF"
                     /locus_tag="M2A35_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCB7200221.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(9876..10133)
                     /gene="trbE"
                     /locus_tag="M2A35_003991"
     CDS             complement(9876..10133)
                     /gene="trbE"
                     /locus_tag="M2A35_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCB7200222.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(10160..11947)
                     /gene="traN"
                     /locus_tag="M2A35_003992"
     CDS             complement(10160..11947)
                     /gene="traN"
                     /locus_tag="M2A35_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCB7200223.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDSGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRTARMELQG
                     STTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQKSVPMTIT
                     VLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMKLRLVVRV
                     KKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSCWAYRDTY
                     VTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGKVMVCGGD
                     VFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAA
                     AGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLEKKRS
                     YCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDL
                     MNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(12258..12638)
                     /locus_tag="M2A35_003993"
     CDS             complement(12258..12638)
                     /locus_tag="M2A35_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200224.1"
                     /translation="MTLPAGYYRIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGAFNSEFQLCFRLQPDAPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(12635..13273)
                     /gene="trbC"
                     /locus_tag="M2A35_003994"
     CDS             complement(12635..13273)
                     /gene="trbC"
                     /locus_tag="M2A35_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCB7200225.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVATTGDCRQVARALLNNPGDKQK"
     gene            complement(13282..14274)
                     /locus_tag="M2A35_003995"
     CDS             complement(13282..14274)
                     /locus_tag="M2A35_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCB7200226.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIAGAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(14271..14903)
                     /gene="traW"
                     /locus_tag="M2A35_003996"
     CDS             complement(14271..14903)
                     /gene="traW"
                     /locus_tag="M2A35_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCB7200227.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(14900..15286)
                     /gene="trbI"
                     /locus_tag="M2A35_003997"
     CDS             complement(14900..15286)
                     /gene="trbI"
                     /locus_tag="M2A35_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCB7200228.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(15283..17910)
                     /gene="traC"
                     /locus_tag="M2A35_003998"
     CDS             complement(15283..17910)
                     /gene="traC"
                     /locus_tag="M2A35_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCB7200229.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(18070..18291)
                     /gene="traR"
                     /locus_tag="M2A35_003999"
     CDS             complement(18070..18291)
                     /gene="traR"
                     /locus_tag="M2A35_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCB7200230.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(18426..18941)
                     /gene="traV"
                     /locus_tag="M2A35_004000"
     CDS             complement(18426..18941)
                     /gene="traV"
                     /locus_tag="M2A35_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCB7200231.1"
                     /translation="MKKISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(18941..19306)
                     /locus_tag="M2A35_004001"
     CDS             complement(18941..19306)
                     /locus_tag="M2A35_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCB7200232.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINIITARSVPDKSVSDDFMNAVL
                     SNCTTRIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVFGQPCAGKT
                     NLTAELISAVVENNKKGEG"
     gene            complement(19245..>19626)
                     /gene="traP"
                     /locus_tag="M2A35_004002"
     CDS             complement(19245..>19626)
                     /gene="traP"
                     /locus_tag="M2A35_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCB7200233.1"
                     /translation="TPAGLYPVRYCSNSFGSDLAMPPSVTCGFRRGDAAEYARETDQS
                     LMQLFTALWATLALMYVSLAVISGKYPMRPGKMKCVRVATADEHLKEVLTEDASLPEK
                     IRKCHVYFPDDRTNRSNGDKNEHA"
BASE COUNT         4647 a         5413 c         4680 g         4886 t
ORIGIN      
        1 agcctgagtc acgccttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gggagagttt
      181 tactgccgga tacggtccgg gcagggtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catatcctta ccggtcgata ccccgtcacg
      301 taccggatca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctgggagaa cggaaaaagg cacgggtgtt
      421 catgacgtca aacagcgtgg cagccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg gatggtaaaa gactcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatag aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tcgggatcag ggtatttgcc gtattatcaa aatccggctg
     1081 tgtcagacac tccttccata aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcgtagtt ggccagacga cggataactt ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttca gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccacac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catataccgg
     1621 ctgggacttg attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc
     1681 gttcacaaac gtctgccagc ttatttttac ccataaaacc agaccaacga gtatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgatattccg
     1861 aagaaaaaaa gtcagtcgga ctgattatta atgatgaaaa gattaataac cgttaaattc
     1921 cggcttcgcc ggcaacactt cacggtcgca caaagaattt gcacaaccgc tcagtgtttt
     1981 catatttatt tcggaaacgc cggggagaaa taaaatttat tttatttctc cccggcgttg
     2041 gacaacgagc aaagcgaggc gtcagtcagg aggccggtca gaccagcctc cggaagataa
     2101 tcagagaata tttgcgattg atttggccag ctggtcttcg agaacaggct tcgcctcttc
     2161 aaatttcagg ttaaccttat tcgcatttga gaccacacgg gtctggtatt tatgctggtt
     2221 acctgtttca gtactggtct gaattttcgc acctgatgtg ccctgacgca gggcggcaac
     2281 attatccgtt gtcaccgttg ccttagtacg ctctgcaatc tgcacatccg tgatcatggt
     2341 atagttcaca tcttccacca tcgcatctgc agccataccc accagaccgg cagcaaggcc
     2401 cacaccgagt gtggcaccgg cagaactgga attatatccg gtgataccgg cacctaacgc
     2461 tgcaccaact gctgcgcctt cataaccacg gttcagccat ccctgagatt cccgcagatc
     2521 catcttatcg gccttcagca cattcgcctg aatccagtag taggctttat ccggagaagt
     2581 caccacctga tatccttttg ctttcacagc atcagcaatt ttgccctgca gcccactcat
     2641 gtctttatca gacgtgtttt tgatctgcag aaataccgtg cgttcgctgg cgggttcaag
     2701 ccagatggtc tcactcatct gagtcttcac cgcaaggtta cgcttcttga ttgctgtgct
     2761 catcgcacca cacccagaaa gggccagagt ggaactgacc agtgcaacca tcatcaattt
     2821 ttttgttttc atcaaattac ctttattaca aaccacagtt ttcttatcgc ctgtcatcat
     2881 agtcctgcca acaagtccca aaagtatttc ctgctgaatc aaatcctcca tgcatagcta
     2941 atcctgttgc cggattagta cgataaaaat ccacataatc aaaattcaca tcatcaaaat
     3001 ccagatgcaa atcttcatta gtttgttcat ttacctgaga ttgcatccat tctcttacac
     3061 ttacacaacc actaattcgc cttaattcgt atagtgtacc aatgataaaa accatcaggc
     3121 ttaataacgg agagagtaag gaagatactg gcatcagtat ataccaaacg cctttactga
     3181 atttaaactc atccgacaca tgccgttttt ttcgtatttg agaaaataac catataaaca
     3241 atccctgcca tagtacaata aagcacagga taaaaactgt ttcggcactc ccccaatcag
     3301 ccagataaca ggcaatgaac tgtatcaccg tcaccagagc aatatgagct aaatttttca
     3361 ttatcatctc cccatacaat attattgctt gtgcttatcc tgatactcct ttgctctttt
     3421 catcagttcc tgtggactgt cagccccggg cattctttcc tgacccgatt tttcttcatt
     3481 atatttatta ttctgagata atgcctctgt tttatggtga ttttgtagtt cagaatactg
     3541 tcttcctaca atattttctt cgccacgtat gctgctctga gtatctccga tattgcctcg
     3601 gtattctgtg accatattat caacctgatg ttttacatca ttacggatat tactgtcctg
     3661 cgttctttgc tcaataatgg cctgatgccc ctgatgatca gcaataatat cctggctgcc
     3721 cccccccgaa ggtacgctct ccattccttt accgatgtcc ccacgggatt cacgccaggc
     3781 attatcaaca ccaggctgca cctgttcctg aacaaaagac cacgccatag cccgacggcg
     3841 ttctgcaatc tccggggaac tggtgtttgt cagaatagct tcggcatcct gcggcgcgtg
     3901 tttcatcaca tactgtgcaa actgttgcga caggtcttca ctcatctgcc cactcatgct
     3961 ttcagtacgg gatgccattt cagcatattc atggctgcgg gtcatattcg tcgtgtactg
     4021 gtcgtaactc tgttttgctg agttcagggc tgcagacagc tggtccacac gggaatcggc
     4081 attattgtcc gtatggctgc cggattcact gaccttgcga ctggtaaagt aatcgctggc
     4141 ctctttaaag tcttgtgtag ccctggcatc gatatcatgg cgagcatcat ggctggcccg
     4201 tgaaccactg ctggcctgat gcgcatcctc atcactccag tcaatacttg cccgaccacc
     4261 acctctaaca cctacgccac caccaacacc aagaatttta ggtttaaccc cccattcagc
     4321 atgagcatcg ccatacatcc ccgcagaacc tctcgtactt cttgatgcca gttctcttgt
     4381 cgcctgctca ttactgatat tatgcgcctt cgcatagctt tccactgcgc tgcgcatacg
     4441 gctggccatc atggagtcct gtgcgctcat cgtgctgtca gcaccaccgg tgacagaatc
     4501 actgctcccc cggttagaac caaactgact gagcgtgttc caggcactgg caatactgct
     4561 gctgaaccca tgcagggcac tttcggctct gttcgacgcc tcccgggcca tttcctgttg
     4621 tgccgccgca atctgacgcg ttgcattgat gccgaccggt aaccgggaca tcgctccgct
     4681 tgcatccatc accatattac cgtcacgggt ctgtgtggcg gttgcgccac tgccggtctg
     4741 gtacatcatc tgaccaaacg acgtggtgct gttggtgctc cagctgaagc cgttcacgtt
     4801 ctccgtctgc atgttgccgt aggagtaatt accgtcaacc acactgcctg ctgcactggc
     4861 ggtcgggctg atagcagagg aagcgaaatg gctgtacaca ctggaaaatc ctgccccgag
     4921 ccccctgacc atcatccagg ataatggcgg gatcatcatg gaaagatacc cggcagtgga
     4981 agccagatcg gagtatttca gctgtatctg tgaaatttcc gagagcacca ccggtgcacc
     5041 attctgcttc gcataaaatg tcatggcgct gttcagaatg gcgtacaaca ttggccatga
     5101 ctgcaaccac atcagggcaa acacatagcc tttcaggacg gacagcgtca gtttgttgaa
     5161 gacagccgcc agtaccagga gcgggaaaat accgatggcg atccccgtca ggatggtctg
     5221 cgtcatgggc agtgtccgca tcgccacatg accgatggag acatgtgcca gacgttgctt
     5281 ctccatcgat gacgtggtgg ccagattcac caggctggct gaatcaccat tacgtgcggc
     5341 ataactggtg atcccctcct tcagcgcatt gatggtgaca ttctggcgga tgatctggct
     5401 ggctgattta cttgagccat agaagtaact gtaactgtcg ccaatcagcg tactgaacaa
     5461 caggttcgga tcagggcgtc caccaaatag ctgctgggca taataatgcc atgtttttcc
     5521 gccactctgt gtgtccagat tcagcttgtc tttcagcgaa accgacgcgt ccttacaggt
     5581 cacgaaatta ttattgctgt cataaacgcc ccgcagcgga ctggggcggg aaaaaatcag
     5641 cgtgtaagga tcagcggagg ccatcagctc ctccagcgta tatttgtggt tcaggtaaat
     5701 atcccccatc acgcagttct gcacataatc ctggaaaaga ttggcgattt ccggattacg
     5761 ggacaaaaag tcggtgcttt ttgataccag ttctgcgcca aacagcatcc ccgttttgct
     5821 gtaggtgaca ctgtccggtt gcgtgaagat catctcgtaa ctggccacca ttgcatgccc
     5881 gatacgggtc gtcagtgaaa gtggcatcgc cagtccgacc ggcacattat ccacccggtg
     5941 aactttgacc aggtcactgt tatcaataat ctgcaccgat gtgcggacat taaccagcag
     6001 gctgataagc acaaacacgg ccacccagcc cagcaaatcc atcacgttgt gccgctgtac
     6061 ccacatcacg gccaccgcga gaacagacaa tgtgagcgca attttttcaa tggaatccca
     6121 cgtcctggtt cccataaagg cggcaatggc gttcaggtta ttccgcaacc actcaccacc
     6181 ggcaatcaca taaacttcat tcacagcgtg ctccctccga agtgatagtt gttctggtaa
     6241 cgactgagca tgcgggcgga aagctgctga cgcatgtagc tcatctgacg atcgacaacc
     6301 agcagcgcat cctgctgtac ctgcacctgt gactgaaacg ccgcaatctg ccgggtggca
     6361 tcattcatgt tgtcgttaat atgttcgata actgcttcgt cataatttcc tgtggccacc
     6421 atcgcccggg cctgctgtat cagctcctga atgtactgca gcaggatgtc gtaaccgata
     6481 tagtccgtca gctggtaaat catactgttg gaaacaccga gcatctgcgg gtcaaccagg
     6541 tatttgaaga cggggatggt ggtactggaa ataaagcctt tttccttgtc atccagaggc
     6601 gtgtcactga ccgccttgtt ctgaatgctg gccagaagtt tagtaatctg agacttcagt
     6661 gcattatccc ggctgatggt caccggtgtg tctgccacca cgttcaggca tttatcagaa
     6721 tcgttgcagt gataaatgtt tgctgtgcca ccttccatca tggcccggat aattgagcgg
     6781 tcagtggttc tggctgacag cggcgtgatt tcgccgttgg gaccaaacac cagtgagccg
     6841 gtcagcgtca tcacaaattc tttcagctct ttgttgccgt caaacattct gtttttggaa
     6901 agcgcattcc acatgatgtt gatgtttctg gtcacccgct ccttatcctt gtcgctggct
     6961 ttatccctga cgctgtcaga tttcccgcca acggtacatc cctgccggga agccgcccag
     7021 tcagcaaaaa tattgctctc accggcaatg tcctgacaga ctttctgctg ggacacctgc
     7081 gtccggggga aaagcccgcc aataatcccc tgtgccgcct ggcaggaact gaggttcata
     7141 ctgttaatgt cgcttgccat tttctgcagg aagtcttttg cggttttgat ttccggcacc
     7201 gttgtctgca aggcaaggtc aaaaaagtaa ccggcagcat tgctcataat ctgcttaaca
     7261 aaacgctgca gctgctcgcc attaataaaa ctgaacgaac cgaggtaggc atcgatgccg
     7321 ccacatccgg cattgatatc cggcagcgtc atggaaatca gctgaacatt tttgacctga
     7381 gtacgggcat acagggagcc gccgtaagca tagccggcag cctgcccctg ccagacgccc
     7441 gactgtgtgg tattggaggc gaagcccagc ttgttaaaga actgattcat gtcgctgttc
     7501 acatccgctg acgctgcagg cgtcaccgcc agtaaggcgg cacaaagaat aaaaagaggc
     7561 ttaatgcgaa gcattattgc tgccctccct taatgtgttc tcccggcgca tacggcgttt
     7621 caacaggaat atcctgatat ttctgactgg taacgcacat aaaggcagca gataacccgc
     7681 aacaaaaaag ccggatgcaa gcgcccataa cgtacacgga atggcataca tcaggatgtt
     7741 ccacatataa cgtacgttat ttaattccgg gtccgctttc cacgaatcaa tacaggcatc
     7801 aaaaataata ttcataacta tccataaaac aattccggca gtcaggaata ccagagacaa
     7861 tacagcatac ctgtcagtaa ttctggaaat gtatttataa tctcgttctg tacttctgat
     7921 tttgagttca ggattatttt tattttcttt cattataagt tccctctgat atttctgata
     7981 atttattcag actggtcatg cagctgtaat atatttcaga cacaaaaatc catcccacaa
     8041 ttgcccccac aaaaaacaca acagaaacag aaaaggtaag gcacagcgaa acgacaattt
     8101 ctacagggct ggcgggataa aaaagcccct gacggataaa ggggatattc aggctgacga
     8161 ttattcccgt cgcgatgccg gatattccgg caatgagtaa caccacctgc ttatttcgca
     8221 cctttttttc ctccgtacat ctgcagaacg gtatccatcc gcgccataaa accggcggca
     8281 tccgttgccc cctgtaaaag cggtaatgcc tcaagcgtgt tgacgttgac caggaaggtg
     8341 gtcggtgtgg ccaccgggat attcgggaag aaggtctgca tcacgtccgg tggcaccggt
     8401 aatgcttccg gaaaggcggt atccccctga ccatccaggg tgtagggaaa aacggaaaag
     8461 ccgtactgct gtgccaactg cttcagtacc gggtcaaact ggtggcagta agggcaatgc
     8521 ccctgcataa acagcaccac cttccagtcg gccaggttaa tctgcctgcc gttgctgagg
     8581 cggaaccagc ggggggccgg cttttctgtc ctggctgacg tgcctgctgc cggttgtgca
     8641 ggctgcgcgg ccataccctg cggattccag agtcgttcaa tttcatcccg ggtggaggcc
     8701 tgcacacctg tgggcatcat caacaaaatg accggcagta atttagtgag agacatgtcc
     8761 gccctcctgc tgctctgcca tcatggcctg ttgcctggct tccagtgcct cacgctcttc
     8821 ccggctgaca cgggcaatcc atgcatccag cacctggtat gcgccgaaca gcacaaggat
     8881 ggccgcaatc aactccgcca gaaaaaaagc catccagggt ttgctgtaaa caagacgggc
     8941 gacgatatga aaccagaccc ccagggaaaa tacccacatt cccgtaatat caagccgggg
     9001 taaagagaat ttatatttat gcacagtaaa actctcctgt catacagaga attaaataag
     9061 ggactaccgg aataaatggt gaattattac caggttcaga attaatggaa tttactgacg
     9121 aacggaaaat atccgtaatt cagaagttcg gtttaaaatc ttcagaaacg ttcaggaact
     9181 gttttgccag gtcgtcctgc gaaataaaac cgtatgataa cggacgaaca ctgccctgtt
     9241 tcgggtcaac cagcatcatg gccgggaaat atttcacgcc gaggcgctgc gcctgcccct
     9301 ggtcagtccg ggaatccggc aacagcggat taatcacgcc atccacggaa acgggaataa
     9361 cactcagacc ataggtatcc ctgaagccat taatgacctg cgccagctgc ccgtcgatgg
     9421 ggtcctgccc ccggtaaaag aacatgatgc cgtagtgttc agccagtttc gcaatggcct
     9481 gtcgctgctg cgcctggtct gccgccagct ggttccggac cgtgccgttg taatggctgt
     9541 actgcaggtt atagtccagt tcgggatgcg ccagcatggc ttttttggcg ctcatggtga
     9601 aaagcccggc ctgctgagtc cagtaattct gcagccggaa atatttcaca aaattatcca
     9661 cgccgggata cagaatagct tcgtacagcg cccgctttgt tgccgtctgc agtgcggcca
     9721 gtttctgcat aatgtccggc tcctgacggg gggctgcagg tactggttta ttttcttttt
     9781 ccttcggatt tattttctcg ttataccact gccagcctgc atcttttcct gacgccggaa
     9841 aaataaagca gcagagtaac agtggcagta atgctttatt catcttcatt cctttcatca
     9901 ctttttcttt catcaggttt ctgcaggtca gtcagggcag aaaaacaaaa atacagaata
     9961 aacaggagca caccggagta aaaaataaca aaacaggcag caagcatctc acctgcagtg
    10021 gtttctttta ccgtatccca cgaccagata attaccgggg aaatgacaat cgctgtaatc
    10081 aaaagccgga taagaaaatc aacaagacga ttagaagtga atataacctt catcatttcc
    10141 cccggttatt ttccctacct tactgtcctg cctgtttcag ctggtcagca atctgctctt
    10201 tcactttctg cgtcagaacg ccactgtccg ggattttctg gttattcatc aggtcttcgt
    10261 agaagttagt gaaatccagt ctgtcgaact gaattttctg cagctcatca accgtaatcc
    10321 cccgacagtc aggatgcttc gcactgccaa aactgatacg cagctgaccg ttgcgcccct
    10381 gctgctggac tatctgcgcc agcttcgaat caaactgaca gtagctgcgt tttttctcca
    10441 gacagacgcc cagcactttc ttcgaacaga actccccgac gctgacggtc agtttgtttg
    10501 attttgcttt agccagggct ttttcatcac tgctgcattt ggccagcccg atatcctgtc
    10561 cccagccgct gtccttacag cagttgctgt atccggctgc agccttctta caaaatttcg
    10621 cctgaccggt gaaagcacgg acatccaccc cgttcagcgc cgcaacatcc ttacctgctg
    10681 cggccagtgc cgccagctga gaaacggctt cggcaaaatc attactctgc ccgctctgag
    10741 ccttatcgca ttcgccatcg agacagaaga cgtctccccc acagaccatc actttcccgg
    10801 atgtttttga ctcacaggag tacgtggcat attcatgcag acaggcacct tcttcggagt
    10861 aaaaggcaca ctggtgtgac accagggtac aggcaggatt gtcggtgtag gtctgacagg
    10921 ttcccttgtc tgctgactgc gtgacatacg tgtcccggta tgcccagcag ctctggtaca
    10981 tgctgtatga ctgaccgtct ttcaccagcg tcctgttccc gccagcctcc gtacattctg
    11041 tttttgtcag tttccccagc tccttgctga acgggcagct ttcagaccag acaacatacg
    11101 gggtgtacga ggcttttttt accctgacaa ccagacgcag cttcattgcc gtatgacgtc
    11161 tgaattttgt attcccgacc cggacggggt gagatgacgt aaaagccaca ccggcagtca
    11221 gtgttttctg tactggtgta aatgaggcat ctgctgagta tttggcattc cagctcagtg
    11281 gggttcccag tactgtaatt gtcataggta cactcttctg caggtaagta cgactccagc
    11341 tgtaatgcgc atcgacaatt tcaccggtaa cgggagaggt aatggagaca acatactgac
    11401 cattcacttc acgtgcaggt agctgactca tttcatactc cagcgtccgg gtttcccatg
    11461 tggtactccc ctgcagttcc atacgggcag ttcgtgtgca gtactgctcc acctgtaaat
    11521 cccgctcaca ggtgtaattc gtgtattcac tccggctaat ctcctgcgca ctgcactgct
    11581 gcccggtgtt tcccacaatg ctgtcagccc ggtttaccac atccctgccg gtctggataa
    11641 acggtgcatc cggtgaaaga atgtctttcg gcttgttcat gaatgactct gtgatggttt
    11701 ttccggtttc accggtcgcc cattccgtgg tgccgtcatt tttcaggccg ctatccccgc
    11761 cggcagtcac gccaccgtaa tattttgtct cgtccggatt cgcgttatag ccggggatac
    11821 tctcctgtgg cttaaagccc tgaatactgc tgcttccctg tcctttgatc tggtgagcaa
    11881 aatcagaacc agcccggtaa tcactgttgc tgtcagcaaa cacagaaaag ctcagaaaaa
    11941 aaacaattgc acaaaaattg cacggcttta cggaataatt aaaattcaga gaccagaaaa
    12001 aggtatttat cttttctttc ataacctgaa ccatactgga gaatgaagtg atgtcatctc
    12061 aacttcagaa ctgtgttgat tgtggtaaag cattaagtcg aacagccaca acgtgcggga
    12121 attgcagctc aacggaccct tttggaaaaa aacggcgcag cgatcgcttt taccagtggt
    12181 ttgcagggct gtttattacg gctattatta ttgctggcgg attatggtac atggggcttc
    12241 ttgacgtacc gggattacta ctcagataac gacttcagta acgatatcag tgttctgaaa
    12301 tcggcacata acgagtgacg acaataccgg ttataccagt aatgaggggc atcgggctga
    12361 agcctgaaac agagctgaaa ctcactgtta aaagccccct tcaccgacca tccccatgtc
    12421 agacgtggta ttgctgattc agcatcctgc cgcagtcgct gctcaagaag ggcagccatt
    12481 tttaccgggc aggcaaaggc aatataagga agcagcttgg taacagggaa accatgcccc
    12541 tgacaggaag catatgtacg gaagccgtga atgttcatcg cagccaccag tgcacggatc
    12601 tccggatcaa tccgataata cccggcaggt aaagtcattt ttgtttatct ccggggttat
    12661 tcagcaacgc ccgggccacc tgccggcagt cccccgtggt ggccactttc tccagcgcct
    12721 gtccgacccg caggtttccc cggatgatgt cgtatccctg actgcagaac accaccagtg
    12781 ccggtacact gcgaatgccg tactgtgaaa acagcgtcgg gtcgatctga acaccatcag
    12841 ttgcgccgtc tttcaccagg gacagcacgg cttcagcggt ggtcttcagg tcattattca
    12901 ccatgccccg cagtgtggcc ggaataccgt agtgccgggt ttcgcccagc attcgtttca
    12961 gcccctcttc gggaatggaa aacgacacaa aatacagagc accctgccgg ggtttcccgt
    13021 cctgcgaagc ctgctgttta cgaaccagct catccaggaa atggttatct gaacgctgaa
    13081 gggggttttc cagcacctgt ttctccgccc aggctttcag ctgatggtca ggtttttcac
    13141 gcagttgtct gcttaaattt tcctgctgct tcaggaactg gcggttttca ggagtgttca
    13201 cgttttctga cgccataacc gccccgttca gcatcatcag cagtgctgcc agagatttca
    13261 tactcagctt catcattcac ctcacaggaa gacacagtta cgtttacgcc acatcaggta
    13321 gccgaagttt ttctttgtat tgggcggatt tttcccggtt tcccagcggg tcacactgcg
    13381 cccgaacggg tggcactgtc cgctgtccgg gtacatattc accatctggt aacgccagcg
    13441 ttctttgggc aggattgggg acggatattc attacagacg gcgttatttt tcccgatggt
    13501 ttccataatc atgccctgac ggtgcagctt gaacgccatg cgttcactga ccagcaggga
    13561 ggactgcaac ggactggact cattactcac ccagccattg aacgggtaca tactgccctg
    13621 cgaaccggca caccagaaca gaacatcgag aggcatatta aaggcgccgg caatggcatc
    13681 tgctgcacag gccccctgtg ctatcggatt ggcaaagatg acagcttccg gattgagaat
    13741 ggtggtcagg ctgctgtccg tccaggtggg gtcgatttca gaaagataag cgatatccag
    13801 gtcaccacct tccagacagc ccagcgatgt gatgatgtta agccagtacg tcagcgggta
    13861 tttgtaccag tgaacgtgat agaacgcccc gttcacctgc ttctcatcct ttttcgccgt
    13921 tccctgtgcc gttttaccaa aagccggcag gctgaagccc aggttcacca tgcatcccgg
    13981 tgaccgggtg acgtccgtca gcgccatcgg ctcccagtaa ccaatggcca gcccgatacg
    14041 cctgaacagc ggcggcggtg ccggacaaat ctgaatgggc atcgacgggt tcgccgtgtc
    14101 ggggacctta ccctgactga ctttgatact gcccagcgag agcgggaaaa tacagctcca
    14161 gcagatatcc gtgatcgggt ttacaaaacg cccctcacag gcagaatccg cagacgctgc
    14221 agggacatga ccggcgagaa ggaataacat cagcagccac aggcttcgct tcattttctg
    14281 ccctcctctg ccggaataaa ttccaccttc aggaagcgat cgccgggaac ggcgctcacc
    14341 cgtgccggga cctgatcaat gccgaggcgc tggcagagca caccgttctg gtcaaagtaa
    14401 acacggctgt ccagagactt ctgcatctcc gggatactgc cctgcacgag gataattttg
    14461 ctctccagtg tgggcggcgt ctggcgtttc atccaggcca cctgcgccgg atcatcgcca
    14521 ttgatgaaat acagcgtctg gttaaaagga acatactgca gcggattcat cacttcaccc
    14581 tgacgggcaa acacccgccc ttcgttatcc cggatatccg cagccagtct gacggacggg
    14641 tcaaacaacc ggctgccgta tttctccgtg cgtccgatac cgggcacggc agggggccgc
    14701 agactgttgc ggatcacccg ctccttaaag gcatccattt tccggcccat ctcaccggac
    14761 tgctccagtg ccgtcaggcg ctgcatgatc accgtcagca tgtccggctc ctttaccggc
    14821 cacagatcgc cccaggtacc aagatcggcg gcggccacac tctgccccca tatcagcagg
    14881 gcaatcagcc cccggcatct catggctccg ccctcattcg ccgggcgata tcctgctgga
    14941 tttcccgggt gatatccgga gccccctgta ccacggcagg cgacaccaga atgaccgcgt
    15001 ggtgtttctg ctgccatgcc tgcaggctgg cttccagtgc cgtgttaaaa cgcgctgaaa
    15061 gggcttttga ctgtgcttct gacagttgtt tctgactggc gctgtcaaaa aacgcatcca
    15121 cggtctgttt catgttgaag gcggcagtca ccggtgcgtt cagtctgaca atgccgtaac
    15181 tgatggctgc gttcagtaac accattgcca ggcatcccgg caccgtccac caccagtggc
    15241 tgcgtcgacg ggctgtaacg tcagtcgttt tttgcgttgt ggtcatgcaa cactcctgta
    15301 tttctcatgt tcttccagcc aggcttccag cgaagccatc tccggccctg acttcttcca
    15361 cgccagctgc cacaccgcct catgaatact cagcccctct ttacgtttct gctgcacaaa
    15421 ctcaaaatcc gggccgtcag agctgtacat ggcgcggctt aacgggtcca caaacaggcg
    15481 gtgccaggag gaatggtttt ccacctgcag caggaaggaa ctgaaccact ggtctttggc
    15541 cgcaccaaac ttaccaatca tgtcgcgctg cagtggcagg aactggtccg gatacaattg
    15601 gttgtatttc gcgaactcct tcgcactctg tttgaggata attttgtagg aggagttacc
    15661 ccatgccgcg cgggcagcac tggaggcctt gtcagagtca aagtcgacga tgttctgtgt
    15721 gatggtgata taggcaccgg tatgacggcg ggccgtacgg tagcctttct caataaattc
    15781 accgaccttg tgatttttga agtccagcag acgccagcct tcatcaataa cgttcagttt
    15841 cttgaggtta cgcggcgtgc ggtacatcct gttctcgatg tagataatca gggagaacat
    15901 caccgcaacc agcagtgacg gacggtcttc cagtccgccc agctccagca ccaccatttt
    15961 tgcgtcatcc cgcagggacg gctcatcaga gttaaaatac tggccgtaag tgccgttggc
    16021 agtgtactgg tcaagcagca caatcatttc gtccagacgg ctacggatgg tcggtgactc
    16081 ggcatactgc tcgctgtcac tggcgttttt caggaaatcc accacgtcat caatacgtgc
    16141 tctgttctct ttggccagcc aggaggctct gaccgcctgc aacagcagac cttcatgcac
    16201 ttcatccagg ttaccgttgg ggctggccat cactgacaac tggtcacgga cacgctccgc
    16261 tgactggtca atatcggtga tgttcgcaaa cgggttaaaa cgcagggttt caccgtccag
    16321 atacaccccg cccatgttct cacacaggga cttgtatcca tcccccatgt cgaacaccac
    16381 agcaaagcct ccggagtcca gcacgctgcg gataagtggc tgtatcagcc cggttttacc
    16441 ggccccggag gtgccacaga ccgccatgtt gtagttggtg ttattcatcc cccggaagaa
    16501 aatatcgata aacgccagct ggttacggta ggtgggtgcc agcagacctg ccggtgtcag
    16561 ggggttatcc gccactaacg gcatcaggtt ggcaacatta aagctctctg cgcgctgaac
    16621 cactccggcc tctttcagct gtttaaacag ccctttcccg gccataaagg gcaggcaggt
    16681 caggaaattg cgcatgtggt taaagcgcgg tgaaatcagc tcaaaaccgt ttttacgaaa
    16741 gctgttcagg atgtcctgct ccacttccag cgccgtttca ttattgtcct tgcagaaggc
    16801 tgtgatgttg aggaagtagg acaccacgga ggactgaccg gagcccagcc gctgacgcag
    16861 ttccccccac tccttcgcct ctttctccac ggacggaaac catttggcat aggaggtctt
    16921 cgactttttc tccaggtcca tgtacttcag gttggcttcg ctgtgggttt tcacctggtc
    16981 ttccaccacc agagtcagcg tcaggatgaa cggacaggag atggacagtt ccgggttcag
    17041 caggttgctg tagttgtcgg ccacgttcca caggaaggcg atttccgggt tacgggccag
    17101 atggaaattc aggatgcggg ccgtgctgtt cctgccgttc tcacgcaggc ccagcgtcag
    17161 gtaatcagcc cggactttca gatcaaaact gtcctccaca cactgataat tcagatcaga
    17221 atacgggtcc agctgacgtc ttttcgggta cagggagtcc gggttatggt taatcatctc
    17281 cccgacaata tcgataaagg cctgtgcatc caccgtctgt gtggtgatac tggccccctg
    17341 taacgacgcc cggatgattt tcaccaggtt ttccatttcc agaatgtcgg cccggctttt
    17401 tttcttcgaa ggagaacagt acgagataaa cacccggtaa tggcgcaggg tcaggggcag
    17461 attcatcccc tccggcagcg gaaactgtgt cgctgccgct ttcatataat aggcccgggt
    17521 aatggcgtta aaccgttcag cctgttcacc tgaccaggag aactcacgca gcccgtattc
    17581 aatcctgtca ccaaccagct gactggacat cagatggatg cagaacggca ccccgcgcgg
    17641 cagtttggtg cgcagcatgt ggtcgagcgc ctccacaatg gactcattcg ccccattgat
    17701 gggaatggct tccagcataa agcccatcgt ggtgtcattc atgaacagac cggattcctg
    17761 gttgtaatca cgatacggca gcagacggct gaactgcggg aagctcattt cgcccagaac
    17821 ttcattggcc tttgcggatt cgtcaggcag tttcagtgct gtgacgaggg agttaaccgc
    17881 ctgagtgacg gcctcaagtg ggttattcac atgaattcct tagttgaaaa ataaagcgag
    17941 aaatgcggta gccatgaagg gaatacctgt aattaacgcg ccatttcccg gcgcatatac
    18001 aaagatttct gttttctgaa tatccattca tgttcatccg gaattaaatc actatgttct
    18061 gcgactgact tatgcataat gttttctctg tctttcctga tacgcctgac attcaacgca
    18121 caacgtcaca ccgggaaata ttttccgccg ggcttccgga ataggatttc cgcatgcttc
    18181 acagagataa acaggaatac catgagcatt tattttctgg cgtatccggt ttattcctgt
    18241 catggtcagt tgttctgtca ctgaatatgc ttcatcggct tcatcactca cggaatattc
    18301 ctcccgacat gttctgacac gctaccgccc atcccttttc acgcacaggg cactcgtttt
    18361 acaggcagat tttgaataca gaaagcccgc cggcattgcc ggctgatata caacttcagg
    18421 gccagttaat taatacgtgg ttttccccac gcagacggtt taataacaaa aaacacgctg
    18481 gatggctgat gataaacatc ctggttatca atataaggcg ctatccataa tgctgccgtc
    18541 tgctcacccg ttcttagcgg acgaggaggt gttaccggcg taactgaact gaccggaaca
    18601 accgttttca cttcccgggc agcggtaaac agcgggcgag gtgccagcag tttctgctcc
    18661 ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg tcgcggtgac ggtctgcacc
    18721 ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg atgccgcaac cggctttgct
    18781 tctgaggact gctccagttt tttggccttc tcattggcct gctccatcgt catacaggta
    18841 tctgatgtgg tggcgttaca ctcaaattcc gtattagttc cggcacagcc agaaagtaat
    18901 aacgtcccca gcagaggaat aaataaagaa atctttttca ttacccttct ccttttttat
    18961 tattctccac aacagcagaa attaactctg ctgtcagatt tgttttccct gcacagggtt
    19021 gtccgaagac tactgtattt cccggagaag aaaataagct catcgacata cccttcttca
    19081 tcacaccgac agctgccata ttaaaattgt gcggcaatga ttcagaaccg aattccttca
    19141 gtgcaggaag tacgattctt gtggtgcagt tgctgagaac agcattcata aaatcatctg
    19201 atacgctttt atccggtaca gagcgtgcag taataatatt aatattacgc atgttcattt
    19261 ttatctccgt tgcttctgtt tgttcggtca tcagggaaat aaacatggca tttcctgatt
    19321 ttttccggca gggaagcatc ttccgtaaga acttccttca gatgttcatc tgcagttgcg
    19381 acccggacac atttcatttt tccgggtcgc atcggatatt ttccggagat caccgccagc
    19441 gagacataca tcagtgccag cgttgcccat aatgcggtga acagttgcat cagagactgg
    19501 tcagtctccc tggcatattc tgcagcatcc ccccggcgaa aaccgcacgt tacagacgga
    19561 ggcatcgcta aatcggaacc aaaactgttc gagcagtatc ggaccggata cagcccggct
    19621 ggcgtt
//
LOCUS       DAHBHC010000089        19581 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHBHC010000089 DAHBHC010000000
VERSION     DAHBHC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.089"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..357)
                     /locus_tag="M2A35_004003"
     CDS             complement(<1..357)
                     /locus_tag="M2A35_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7200234.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLAMVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTL"
     gene            complement(357..572)
                     /locus_tag="M2A35_004004"
     CDS             complement(357..572)
                     /locus_tag="M2A35_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCB7200235.1"
                     /translation="MKSMDKISTSIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            complement(865..939)
                     /locus_tag="M2A35_004005"
     tRNA            complement(865..939)
                     /locus_tag="M2A35_004005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Gly,seq:tcc)
     gene            complement(943..1018)
                     /locus_tag="M2A35_004006"
     tRNA            complement(943..1018)
                     /locus_tag="M2A35_004006"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(983..985),aa:Thr,seq:tgt)
     gene            complement(1532..1795)
                     /locus_tag="M2A35_004007"
     CDS             complement(1532..1795)
                     /locus_tag="M2A35_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit B"
                     /protein_id="HCB7200236.1"
                     /translation="MKKMIIAVLFGLFSANSMAADCAVGKIEFSKYNEDDTFTVKVSG
                     REYWTNRWNLQPLLQSAQLTGMTVTIISNTCSSGSGFAQVKFN"
     gene            complement(1808..2767)
                     /locus_tag="M2A35_004008"
     CDS             complement(1808..2767)
                     /locus_tag="M2A35_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit A"
                     /protein_id="HCB7200237.1"
                     /translation="MRHILLKLVLFFCVCLSSASYADEFTVDFSSQKSYVDSLNSIRS
                     AISTPLGNISQGGVSVSVINHVPGGNYISLNVRGLDPYSERFNHLRLIMERNNLYVAG
                     FINTETNTFYRFSDFSHISVPDVITVSMTTDSSYSSLQRIADLERTGMQIGRHSLVGS
                     YLDLMEFRGRSMTRASSRAMLRFVTVIAEALRFRQIQRGFRPALSEASPLYTMTAQDV
                     DLTLNWGRISNVLPEYRGEEGVRIGRISFNSLSAILGSVAVILNCHSTGSYSVRSVSQ
                     KQKTECQIVGDRAAIKVNNVLWEANTIAALLNRKPQDLTEPNQ"
     gene            3256..4176
                     /locus_tag="M2A35_004009"
     CDS             3256..4176
                     /locus_tag="M2A35_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200238.1"
                     /translation="MIFLRTENGIEKLENWEEIISRPNFVTTIDKGVQQLEEIIGYYK
                     FKEEIHCGLTGCNQPHQMGYIVKTSSGIETNIGNKCGKNEFGVEFGENVLSFNKFMQL
                     ETNREIISTAKSKCEEWQKNIETIRNIKPTIDYLSSTIENSKNSNYSGRLGATEIRFL
                     TKSQTGLVTLSEVETDKNTKTILFAMNEHMRESGEAISEFYIGKVSFSHVLLPENNLR
                     DFFVALKEDLKKIQCIDLQTAPSPEISNVAQIASSIEERIKRLKKLKHEASKFLTKKN
                     LRPIANKIKYSSTADELEFHNFERFLNGLK"
     gene            complement(4291..5121)
                     /locus_tag="M2A35_004010"
     CDS             complement(4291..5121)
                     /locus_tag="M2A35_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7200239.1"
                     /translation="MKLEASLKYFSPQGMYIGDDVKETSPERLTGTDVMAAIGTTSNR
                     ERFGLAAFFGKAGISKTDEQMAVQALARHAMEIAPKNVRKAAGGEFGWCMLVLAQFAF
                     AEYSRSAATSVTCHSCSGTGLTPRKQVIRKVSYPWGKAPYWASRSRAVRPSDWEKWTE
                     VTEVVLVVCDVCRGKGVISARCRCGGKGEVLDRKATSERGVPVFKICERCSGNGFSTV
                     PSTTVYKVVLKRIPELHVRTWTRNWKPFLDSLVDICHQEERKADIAFQNVTSFGDDVN
                     "
     gene            complement(5121..5978)
                     /locus_tag="M2A35_004011"
     CDS             complement(5121..5978)
                     /locus_tag="M2A35_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200240.1"
                     /translation="MRTDNNEHKALFTIPTAAYSSALANIKPLPEQRRITGHKQTDAY
                     LWVLEVIRLNEPAHLDAAEAALVKIKISPKEAQERYSRYLLANGGDPFQIAFGTIGMD
                     NPAQAIRIARENIKKATSVRATFGSYEAALEDVEAERVIKSSQKFIDDHLWGWTAAEK
                     TAGRIDGIRMNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFVYWDWLYSVRHTASKEQ
                     GDEYGYSEHHESVYDRERYLEKLLMTIKPVTRAEAVEVCRWFLASGKGEYMKDNGATV
                     ILNLVGECE"
     gene            complement(5978..6946)
                     /locus_tag="M2A35_004012"
     CDS             complement(5978..6946)
                     /locus_tag="M2A35_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCB7200241.1"
                     /translation="MKTAAAAKGRWPEILEHFGLPPITGKNHFKGECPVCGARGKFRI
                     DDRDGAGTWICVCGSGDGMKLVTLTQAKPFNEICTEIDRLIGNDYQRVKIPVTSSATS
                     LRKRVLSKFSKLEALRGTSGASYLNSRGIFSLPAEAIRFNARQRHNGSVFQSLYSLAT
                     DDKGELCYLHQTLLDGDKKADIGSSAKRLKSLQEDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYNVNTWATINSGFMKKFRVPAGVLHLIIFADRDENSATGLAAACEC
                     AHANLMAKNDLQRVSVYWPDHDDFNNMLMNGDQVRELVFHKKKAVA"
     gene            complement(6948..8606)
                     /locus_tag="M2A35_004013"
     CDS             complement(6948..8606)
                     /locus_tag="M2A35_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCB7200242.1"
                     /translation="MQLTITPNFAQERALNMLRRDWKANDTFMVYSPTGSGKTGLAAF
                     IVAGFVSRGMRVLFCAPYTILIGQTANRFVEYGLPGDEIGYIWADHPNYDPDRKIQIA
                     SADTLIRRVFPENIDLLIIDEAHLRKKRILKDIERLRGKGVKVIGLSGTPFSPFLGKY
                     YDRLIKPTTIGELIQRGDLSKYEFYAPTKPDLKGVKTTSSLEYGRDYNETQLAEIMCG
                     STLVGDIVQNWLENGRDLPTIAFCVNVAHANYLTIQFNLAGVNAEVMTADTPVDERQT
                     IIHRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQALGRGLRTAPGKES
                     CLIFDHSGTVHRLGYPDSIEYDDLPGKSDGMEESARRAVEERAEKLPHECPQCHYMKP
                     AGVYVCPKCGHKPLRGEDVDTDTSRKLNKLGKNQHQSTKAEKQSWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEISPEVSNYIKHKLIRFAKGRQRTQRTI
                     EKLQATIPLSQERGERSEMPIGTEAWRIMQAKQQLQKNINSLSQ"
     gene            complement(8718..9086)
                     /locus_tag="M2A35_004014"
     CDS             complement(8718..9086)
                     /locus_tag="M2A35_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200243.1"
                     /translation="MSEDLKQNLIALLEEQFIRSDDKVVFDYVMQKKIKSQGYHLQRN
                     FSISISGGRKGFIDCLVTSSDGQQCAIEVDKKSPRNRSLMKLAQLPEGMSGFVLLRDG
                     KHPLRYSENGIDVIRATKFK"
     gene            complement(9591..9878)
                     /locus_tag="M2A35_004015"
     CDS             complement(9591..9878)
                     /locus_tag="M2A35_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200244.1"
                     /translation="MKHKAIEKAIEILGSQQALAKRCGKAQSTVCDWLNCKNKISPEF
                     VPLLVAAVDGKIQAYEFRPDLPELFPHPSLARTGEVKGLYVPELRSDRDVI"
     gene            9998..10690
                     /locus_tag="M2A35_004016"
     CDS             9998..10690
                     /locus_tag="M2A35_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7200245.1"
                     /translation="MTFSERLDLAMRNAKFTQGRLAKEVGMAQSSVNQLLNKANGSRK
                     TVEIAKVLGVNPEWLASGVGPMEIVTSADSHQIRNISEDWVTDSYVVDVLDIRYSCGP
                     GSYNSDFPDIVRSIAIEPGYASRVFGGRPASAIKAINAHGDSMKGTIDPEDLVFVDVS
                     VRTFNGDGIYAFTYSGTSHIKRLQKIKDTLTVISDNPAYKDWAIEPEDFEQLHIDGKV
                     IVSWPMTLHRFA"
     gene            10835..11332
                     /locus_tag="M2A35_004017"
     CDS             10835..11332
                     /locus_tag="M2A35_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200246.1"
                     /translation="MSSYMGLPTNQHDALENIQLLVGAGALLYSSNNPELIELASSIL
                     AVTQKYSLTASRLCKQNLAINSQNRIRTQRETCGLTTAELARLLELDEEIIIQWESGE
                     YEPTISMLIPLANVLGCDPMWLLTGEVTPPEQPKSEEQQHHDASQQVCSLSREALLRK
                     NQYQW"
     gene            11620..11826
                     /locus_tag="M2A35_004018"
     CDS             11620..11826
                     /locus_tag="M2A35_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200247.1"
                     /translation="MTETTSQQDDYEGICLEPDSFAVNVYHLLHATQVLHMSSHHETK
                     TLGSEILNFTCEYAKAAAEKELAQ"
     gene            12049..12237
                     /locus_tag="M2A35_004019"
     CDS             12049..12237
                     /locus_tag="M2A35_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200248.1"
                     /translation="MQDVPEGVIHEKIRVLLTIENGKVIYSKHLLDNEFVGCMDTFLW
                     MAKRAGYTIIPPAKEQTL"
     gene            12234..12815
                     /locus_tag="M2A35_004020"
     CDS             12234..12815
                     /locus_tag="M2A35_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200249.1"
                     /translation="MNHSEFRPEVTPHGIKIGNTTIDYVEAVQRLNDGEYDYPNSHSL
                     RIMQCIAEADDAGLLGRFSVDMKIAQWRWLYVTTFINEEEGKNGTIDIPNDNGTTDRA
                     VIYKGKHGCLSIYPGPLRIALQNHVEWGFIEKYGEAEGMGRVLFLYQKMLIADPDNGF
                     IVSAMGREGLELLLDEMIHDLNTHGIPEAPVTH"
     gene            12823..13101
                     /locus_tag="M2A35_004021"
     CDS             12823..13101
                     /locus_tag="M2A35_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200250.1"
                     /translation="MANQNKINVFQVESKHKTPVIKHVRRHTRIYTPEEFMAMPMIKK
                     LIRDNPGLVAIDKNSGEIMLSREIAEIYCSVNNSKKLKKAIRKISGGA"
     gene            13283..14110
                     /locus_tag="M2A35_004022"
     CDS             13283..14110
                     /locus_tag="M2A35_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCB7200251.1"
                     /translation="MKKIIFSLAIVLPAIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDDPFSFQMSDGTTIGYRIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDSGKSELLDAALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSDANTPFIQVK"
     gene            14151..14510
                     /locus_tag="M2A35_004023"
     CDS             14151..14510
                     /locus_tag="M2A35_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCB7200252.1"
                     /translation="MNTHNIQPQIMNYDPNLTLCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERDIIATINIGGLECKDEKLSGELWLAGM
                     LISAEIISIEPATNIRL"
     gene            14542..14739
                     /locus_tag="M2A35_004024"
     CDS             14542..14739
                     /locus_tag="M2A35_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCB7200253.1"
                     /translation="MFALINQGQLYTDSAGYPVKIIRCINNTVLYRRMDGQMQSVKIN
                     DFNGLFERIDHLEYRQILAET"
     gene            14788..14934
                     /locus_tag="M2A35_004025"
     CDS             14788..14934
                     /locus_tag="M2A35_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200254.1"
                     /translation="MNKAFEQWVHQRYGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            14943..15179
                     /locus_tag="M2A35_004026"
     CDS             14943..15179
                     /locus_tag="M2A35_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCB7200255.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRARKECWMVGREYI
                     HVSPDGNPKPSSECMYNRKAIDAWVASMKNKQPG"
     gene            15235..16548
                     /locus_tag="M2A35_004027"
     CDS             15235..16548
                     /locus_tag="M2A35_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCB7200256.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVSKKEITVKELEEKWLDLKRMEISAN
                     AFNRYESVARTMVPKIGGSRLVSSVTKEELLYIRKDLLTGYQNSTKNKAAAKGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQ
                     TKNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVV
                     HLIQPAISVLKNQAEMTRLGKQHHIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFI
                     YKVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASALMVF
                     NVYGAWMTDSNAEQIAMLNQKLADYVPMMSHGHQSDTRDLLKSVG"
     gene            16530..17300
                     /locus_tag="M2A35_004028"
     CDS             16530..17300
                     /locus_tag="M2A35_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCB7200257.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAACPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            17353..17748
                     /locus_tag="M2A35_004029"
     CDS             17353..17748
                     /locus_tag="M2A35_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCB7200258.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            17789..18532
                     /gene="cmoA"
                     /locus_tag="M2A35_004030"
     CDS             17789..18532
                     /gene="cmoA"
                     /locus_tag="M2A35_004030"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCB7200259.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            18529..19500
                     /gene="cmoB"
                     /locus_tag="M2A35_004031"
     CDS             18529..19500
                     /gene="cmoB"
                     /locus_tag="M2A35_004031"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCB7200260.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
BASE COUNT         5475 a         4664 c         4411 g         5031 t
ORIGIN      
        1 aagcgtcgat gttctgaaat tgccagcgcc cacgttgtaa acgaacgagt aaagagcgcc
       61 gcgcgttgtt tccggtatat cgactttgat gtacgggtta atttgtctgg cgaccatggc
      121 aaggtcttta ttcaggaggg ctttgcattc tgcttcggta tacgttttac cgggaatgat
      181 gtcttttccg gtgtgtccgt gacatacagt ccatacgcca acgatatctt cgtatggtat
      241 gtagctgaca ccttccaggc catcgtcacc actcggacca gtgatgagca cagacgctat
      301 ggcaatagcc ccgccaccaa tagcagctgc aacagccttg cgtaatgatg gaggcattat
      361 tcacctctcg cagcctttct tctgtcttct ctgattttga agtacagatt tgtcagataa
      421 gtcaggaagc ccagaaccag actccccagt acaccaatcg cagcccactg tgacggactg
      481 acctgatcaa gccactgtaa aaaccagtag ccagcactgc ctgcggaggt gccgtaggca
      541 atgctcgttg aaattttgtc catggatttc atagcctcac ctccgcaaat aacggatggc
      601 gtagttttac actgagaaat gaaagggatt tgaaaagaaa aaaccgcaaa agcgggcgaa
      661 acgatatata cagtaaggaa agcactctat ccaacaaacc acccacagtt aatcggaata
      721 aaagcagagt gcttatgaat gatcgcctgc ccgaaagtta gtatttctgc acaacaattt
      781 tgcaaaaaaa agcgatcatt cataacttaa acgtctttca gtcactccgg gatttcccat
      841 catcgcagac tgaaagactc taactggagc gggcagcggg aatcgaaccc gcatcatcag
      901 cttggaaggc tgaggtaata gccattatac gatgcccgca tatggtgccg actaccggaa
      961 tcgaactggt gacctactga ttacaagtca gttgctctac ctactgagct aagtcggcgc
     1021 tggttcctcg ggtgtgaatg agatgcccgg atacataatt gacccttccc tcggaaccaa
     1081 agaaatcata ctgataaata aacagagttc aatgtctgct tattcatctg ttgaaaaaac
     1141 actcagaatt acagagcgat attcacactc accacgattc tgacataccg cccgattttc
     1201 gaagcgtgag ggacacgcaa gcagtacatg tccggtattt cagcatcccc atcagttaac
     1261 atttaaagca cataacaatg tatacgagca tcctaattga tgcttctttt atggaacgta
     1321 ggtattgcct taagggtaaa ccggcaagtg ctgagttatt tgagcaaagt aaagtgatga
     1381 tcttcaccca actacagacc aaaaaggggc gtgaacgccc ctgtatttat atgactttaa
     1441 cgaaaacact gaccaaattt aatcagcatc cacaacacta ataatatctg cgtccagaaa
     1501 caaaagacgc gcataaataa accgtagatt ctcagttaaa cttcacctgg gcaaagcctg
     1561 agcctgaact gcaggtatta gatatgattg ttacagtcat ccctgtcagc tgagcacttt
     1621 gtaacaatgg ctgcaaattc catctgttcg tccagtattc tcttcctgac accttcacag
     1681 taaaggtatc atcctcatta tacttggaaa actcaatttt tcctacagca caatccgccg
     1741 ccatggaatt agcagaaaag agaccgaata aaactgcaat aatcatcttc ttcatattca
     1801 ccccctgtta ttggtttggt tcagtaagat cctgaggctt gcgatttaat aaagcagcga
     1861 ttgtattcgc ttcccacaaa acattattta ctttaatggc cgccctgtct ccaacaatct
     1921 ggcattctgt tttctgtttt tggctcacgg aacgaactga ataacttccg gttgagtggc
     1981 aattaaggat gaccgcaaca cttccgagaa tcgcagaaag actattaaaa gatatcctac
     2041 cgattcttac cccttcctct cctctgtact ctggaagaac attacttatt cttccccagt
     2101 tcagggtaag gtcaacatcc tgagccgtca ttgtataaag cggagatgcc tcagacagcg
     2161 ccggtcggaa tccccgctgt atttgtctga atcgcagagc ttctgctatc actgtgacaa
     2221 aacgcagcat agctctggat gatgcgcggg tcatggaacg tcctctgaac tccattaaat
     2281 ccagatatga accaaccagt gaatgacgcc caatctgcat ccctgtacgt tccagatctg
     2341 cgattcgctg taatgatgaa taactgctgt ccgtcgtcat ggaaacagtt atcacatcag
     2401 gcactgaaat atgtgagaaa tcggagaatc tgtaaaaggt attcgtttca gtattaataa
     2461 agcctgcaac atataagtta ttccgttcca ttattaaacg gaggtggtta aatctctcgc
     2521 tatatggatc aaggcctcta acattcaatg atatatagtt tccgcctgga acatgattaa
     2581 ttactgaaac agaaacacca ccctgagata tatttccaag tggagtggat attgccgacc
     2641 ttatactatt caatgaatca acatagctct tttgcgaaga gaaatccaca gtaaactcat
     2701 ctgcataaga tgctgaagac aagcaaacac aaaaaaacaa caccagcttt aataatatat
     2761 gtcgcatata ttaacactcc atcagtctga accggcagcg ccaggaataa tacctcgcaa
     2821 gaagtccgga atgtttttag cagaaattta ttacaaacaa atcccctcac aggaaatgac
     2881 tgcacaatgt attacttccc tgtttatcag gaacagaaag cagataatga tgcactatag
     2941 cttccggaaa atagtatcaa ttatttaaga ttacgggtat atggtttata tttttataga
     3001 atagagaatc tctcacatat aaagaacacc ataaacttta cttcagccac agcctggtca
     3061 agaggagcgt ccattgacta tgcccccccc tggcaccacg accggtttac ggacataaaa
     3121 aaaccgcccg gcggcgtgtt taagctgtgt ggcgaagtta cccatcttta ccaagtattc
     3181 ggtaataaaa taacttgtgg tgttctcatt tttatgccga taaaataaac ggtccacttt
     3241 caaatggagc gattcatgat ttttctcaga actgaaaacg gcatcgagaa acttgaaaac
     3301 tgggaggaaa ttatatccag accaaacttt gtcaccacta tagacaaagg agttcaacaa
     3361 cttgaggaga taattggtta ctacaaattt aaagaagaaa tccactgtgg tctaactggt
     3421 tgcaaccaac cacatcaaat gggttatatt gtaaaaacat ctagtggcat tgaaaccaac
     3481 atcggaaata aatgtggtaa aaacgaattt ggtgtggaat ttggtgaaaa cgttcttagt
     3541 ttcaataaat ttatgcaact cgaaaccaat cgggaaatta ttagcaccgc aaaaagtaaa
     3601 tgtgaagaat ggcaaaaaaa tattgaaacg atacgaaata tcaaacccac aatagattat
     3661 ctatcttcta ccatcgaaaa tagtaaaaat tccaattact ccggaagact tggtgctaca
     3721 gaaattcgtt ttttgaccaa gagccagaca ggccttgtaa ccctttctga agtggaaact
     3781 gataagaata ccaaaacaat tctgttcgca atgaatgaac acatgcgcga gtctggagag
     3841 gcaataagtg aattctacat aggaaaagta tcatttagtc atgttctttt accagaaaac
     3901 aatcttcgtg atttttttgt tgcgctcaaa gaagatctaa aaaaaattca gtgtattgat
     3961 ctacaaacag ccccaagccc agaaatttct aatgttgccc aaattgccag ttcaatcgaa
     4021 gaacgaatca agcgattaaa aaaactgaaa catgaagcca gtaaattcct aactaaaaaa
     4081 aatctgcgtc ccatcgcaaa caaaataaaa tactcatcga cagcggatga acttgaattc
     4141 cataatttcg aacgcttttt aaacgggctg aaatgataaa agccccgaaa ggggcttcta
     4201 tcaaaatcat ttacgcatta ttccgcatgc ttaaaagaat acatgacaga ttcggacaaa
     4261 atcaagtgca atgtcgcaaa aaccctaagc ttagttcaca tcatcaccaa aacttgtcac
     4321 attctgaaaa gcaatatcgg ccttacgctc ttcctggtgg caaatgtcaa ccagagaatc
     4381 aagaaacggc ttccaattac gcgtccatgt cctgacatgt agctcgggta tccgcttcag
     4441 tacaacttta tatactgtgg tagacggtac cgtggaaaat ccatttccgc tgcaacgttc
     4501 gcagatttta aacactggca caccacgctc gcttgtggct ttgcggtcca gtacctctcc
     4561 ctttccacca caacgacacc tggcacttat cactcccttc cctctacata catcgcaaac
     4621 gactagtaca acttccgtta cctctgtcca tttctcccaa tctgacggtc gaacagcacg
     4681 ggagcgactg gcccaatatg gtgctttacc ccatgggtat gaaaccttac gaatgacctg
     4741 cttacggggt gttagtccgg taccactgca actatggcat gttacgctgg tggccgccga
     4801 tcgggaatac tcagcaaaag caaactgcgc cagtaccagc atacaccagc caaattcacc
     4861 accagctgct ttacgcacat tcttcggtgc aatttccatc gcgtgacgcg ccagcgcctg
     4921 gactgccatc tgctcatcag tcttgctgat accggccttc ccgaagaagg ccgccaggcc
     4981 aaaccgctca cgattgctgg tggtaccaat agccgccata acatcggtgc ctgtaagacg
     5041 ttccggagag gtttctttca catcgtcgcc gatatacatt ccctgaggac tgaagtattt
     5101 tagcgatgcc tcaagcttca ttattcacac tccccaacca gattaagaat aaccgtcgcg
     5161 ccattgtctt tcatatattc gccctttccg cttgccaaaa accaacgaca cacctccacg
     5221 gcttcagccc gtgtcaccgg tttgatggtc atcagcaatt tttcaaggta gcgctcgcgg
     5281 tcatataccg attcgtgatg ctcagagtaa ccatactcat cgccctgttc tttagacgca
     5341 gtgtggcgaa cactgtagag ccagtcccag taaacaaact cacgaactac gtctgacaat
     5401 gtatgaggct ctggcagtac atcacgatag ccatcaacat atgcacgacg ctgatcatca
     5461 atttcattca tacggatgcc atcaattctg cccgctgtct tctcggctgc agtccagccc
     5521 cagagatgat cgtcgataaa tttctgggaa gacttgatca ctcgctcggc ttccacatct
     5581 tcgagtgctg cttcatagct accaaacgta gccctgactg atgttgcttt tttgatattc
     5641 tcccgggcga tcctgattgc ctgtgccggg ttatccatgc cgatggtacc gaaagcaatc
     5701 tggaaaggat caccaccatt cgccagcaga taacgcgaat agcgttcctg ggcctctttt
     5761 ggggaaattt taattttcac cagcgcagcc tcagcagcat ccagatgtgc gggttcgttc
     5821 agacggataa cctccagcac ccaaagataa gcatcagtct gcttatgccc ggtgattctc
     5881 cgttgctctg gcaggggctt gatgtttgcg agggcggaac tgtacgctgc cgtcgggatg
     5941 gtgaatagtg ctttatgttc gttgttatca gtacgcatta cgcaaccgcc tttttcttat
     6001 ggaaaaccag ctctcgaacc tgatcaccgt tcatgagcat attgttgaaa tcatcgtgat
     6061 ccggccagta cacgctcacg cgctgcaggt cattctttgc catcagattg gcatgagcac
     6121 attcgcaagc cgcagccagc ccggtggcgc tgttctcgtc acggtcggca aaaataatca
     6181 ggtgcagaac accagctggt acgcggaact ttttcataaa gccgctgtta atggttgccc
     6241 aggtgttcac gttataaatc tggtgcgctg acagcgctgt ttcgatgcct tcggcgatac
     6301 ccagagtgct agcaacagga aacatgcgga tagctacaga acgagcgtga tccaaatagt
     6361 tatcttcctg cagggatttg aggcgctttg cactgctacc gatatctgct tttttatcac
     6421 catcaagcag agtctggtgc agatagcaca actccccttt atcgtccgta gcaagtgaat
     6481 aaagagactg gaacacactc ccgttgtgtc tctgcctggc attgaaccgg atcgcctcag
     6541 caggaagact gaatattcca cgagaattaa gatacgatgc gccggatgta ccacgcagtg
     6601 cctccagttt tgaaaacttg ctcaataccc gtttgcgtaa gctggtggcg ctgctggtta
     6661 ccgggatttt aacccgttgg taatcattac cgatcaggcg gtctatttcg gtacaaatct
     6721 cgttaaatgg cttcgcctgt gtcagggtga caagtttcat accatcgcca ctaccacata
     6781 cacagatcca cgttcctgca ccgtcgcggt catcaattcg gaacttgcca cgtgcaccgc
     6841 ataccgggca ttcacccttg aagtgatttt ttcctgttat cggcggcaag ccgaagtgct
     6901 ctaatatttc aggccagcgg cctttcgctg cagctgctgt tttcatctta ctgactcaaa
     6961 ctgtttatat ttttctggag ttgttgcttt gcttgcatga tgcgccaagc ctcagtgcct
     7021 attggcatct cgcttcgctc acctcgttcc tgagacagag gaattgttgc ctgcaatttt
     7081 tctatagttc tctgcgtccg ctgacggcct ttggcaaacc gaatcagctt gtgtttgatg
     7141 tagttactta cttctgggct tatttccatc gggaaactgc tcaacccatc aggccattca
     7201 ccgaatttct ccctgaaagt gtgagcacac catccatcac tgactggacg ccccagcgaa
     7261 gcacgctggc gctgataaaa tttgatctga ctccaccagg actgtttctc tgccttcgtc
     7321 gactgatgct gatttttacc cagcttatta agtttgcggc tagtgtcagt atcaacatct
     7381 tcacctcgca gcggcttgtg tccacattta gggcaaacat agacgccagc tggcttcatg
     7441 tagtggcatt gagggcattc atgtggcagt ttttcggccc gttcctcaac tgcgcggcgc
     7501 gcgctttcct ccatgccgtc agacttaccg ggaagatcgt cgtactcgat tgaatccgga
     7561 taacccaaac ggtgcacggt gccgctgtga tcgaagataa ggcaggactc tttacccggt
     7621 gcggtgcgca gcccacgccc gagtgcctgc aaccagcgaa tttcgctttt tgttggcctg
     7681 gcgtagatga tgcaacgaac gtcactatca aagccggcca ccagaacgcc cacactaacg
     7741 atgattttcg ttgcaccagt ttcaaagcgg tgaatgatgg tctggcgctc atctaccgga
     7801 gtgtctgcgg tcattacctc agcgttaaca cctgcgaggt taaactggat tgtcaggtaa
     7861 ttggcgtggg ctacgttgac gcagaaagcg atggtaggta gatcccgacc attctccagc
     7921 cagttctgta cgatgtcgcc caccagcgta gagccgcaca tgatttcagc cagctgtgtt
     7981 tcgttgtaat cgcggccgta ctcaagcgaa gatgtggttt taacaccttt cagatccggc
     8041 ttagttggcg cgtaaaattc gtatttactc agatcgccac gctggattaa ctcgccgatg
     8101 gtggtcggct taatcagtcg gtcatagtat ttgcccagga acggggaaaa cggagtaccc
     8161 gacaggccaa tcacctttac gcctttaccg cgcagacgtt cgatatcctt caggatgcgt
     8221 tttttacgca ggtgtgcttc gtcgataatc agcagatcaa tattttcagg aaaaacacga
     8281 cgaataagcg tgtcggcgct ggcaatctga attttccggt ccggatcgta gttcgggtgg
     8341 tccgcccaga tataaccgat ttcatccccc ggtaatccat actccacgaa ccgattagcc
     8401 gtctgaccga tcaggatggt gtacggtgca cagaacagga cgcgcatacc acggctgaca
     8461 aacccggcaa cgatgaaggc ggccaaaccc gttttaccgc taccggttgg cgagtacacc
     8521 atgaaggtgt cgtttgcctt ccagtcacgg cgcaacatgt ttaacgctcg ttcctgtgca
     8581 aaattcggcg tgattgtcag ctgcattgtg ctgtccccgc agtgatgaga taataatttt
     8641 gtgatgtggt tttcatggat tcctcctcac atggctggtg gtctccccaa aggctgccag
     8701 cccctttccg aatcaactca cttaaatttc gtcgcacgaa taacgtcaat tccgttctcg
     8761 ctatatcgaa gagggtgctt accatccctg agcaggacaa agcctgacat cccctcaggt
     8821 agctgagcca gcttcatcaa cgaacggttg cggggagact tcttatcgac ctcaatggca
     8881 cactgctggc cgtctgatga tgtaaccagg caatcaataa accctttacg accaccgcta
     8941 atgctgatgc tgaaattgcg ttgcaggtgg tatccctgag acttgatttt tttctgcatc
     9001 acataatcga aaacgacttt gtcatcggag cgaatgaatt gctcttccag gagagcgatt
     9061 aaattttgct tcaggtcttc actcatactt tttatccatc ttgtactagc tttatggtac
     9121 ggccgttttt tcaggggtat ccccctttag atcgagatct acctaaccta tgtacccgtc
     9181 tgttggaaaa gcctgttcca gtgcttcgca ctaacacacg agcactcctc cccctcccct
     9241 cctctcactg aattttgcat gtactcctta gctagtacac aaatgcaatg agatttggga
     9301 ttcagccacc ggacaccttt aagcccggta ccaatcagga gcgcgattgc gttcctgcca
     9361 ggggcggctg agttgtatac ccctgtaaag ctctgccctg atttctcaca aacaagcgga
     9421 gccttgtgtt tgcttcgtgc cttgctctgt tctccttgcg gaatgaaaca ggctcagcgt
     9481 caaaagtgat ttcgtatacc tccgcatatt tcagggcgac cttccgtctc agtgacggag
     9541 gcagcccctg taactgctgc tgaatccact cttcgtctgc ctggcagtac ctagatgaca
     9601 tctctgtctg aacgtaattc tgggacatac aagcccttca cctcccccgt acgcgctaag
     9661 cttggatgtg gaaaaagctc tggtaggtct ggacgaaatt catacgcttg gatctttccg
     9721 tcgacagctg caaccaataa cggcacaaat tctggtgaaa tcttgttctt gcagttcaac
     9781 caatcgcaga ctgttgactg ggcctttccg catcgcttgg ctaaggcttg ctgacttcca
     9841 agaatctcaa tagctttctc tatggcttta tgtttcataa tcgcatctcc tattgaacgc
     9901 ataataacaa caggaaaagc gatattcaac gttaaaccta gaatatttat cggaaagccg
     9961 ataaactagc caagatagag aggggaggaa aatcgcaatg acattttcag aacgacttga
    10021 tttagctatg cgcaacgcaa aatttacgca aggtaggctg gccaaagaag tgggtatggc
    10081 tcagtccagt gttaatcaac tacttaacaa agccaacggt tccagaaaaa cagtcgaaat
    10141 agcaaaagtt ttgggcgtta acccggaatg gctcgcatcg ggtgttggtc caatggaaat
    10201 cgtgacctct gcagactcac accaaatacg taacatttct gaagattggg ttactgattc
    10261 gtatgtcgtg gatgttttag acatcagata tagttgcgga cctggtagtt ataactctga
    10321 ttttcctgac attgtcagat ctatagcgat agaaccagga tacgcatcaa gagtttttgg
    10381 cggcagacca gcatcagcaa tcaaagcaat aaacgcccac ggcgacagta tgaaaggcac
    10441 aatagaccct gaagacttgg tatttgtaga tgtatcagtt cgcacattta atggtgatgg
    10501 tatatacgcc tttacatact ctggaacatc gcacatcaaa agacttcaaa aaatcaaaga
    10561 tactctaacg gtgatatctg acaacccagc ttacaaggat tgggctatcg aaccggaaga
    10621 cttcgaacaa cttcacattg acggaaaagt aattgtcagt tggcctatga cactacaccg
    10681 ttttgcataa gcaaaaatca tttaacccgc ccactcttaa gtgggctttt ttttgggaaa
    10741 aaaatcgata aaccgattga cagatgaaat cggtaaacct attatcttga cataggtatc
    10801 gaactcacca aagcaattat agaggatgag aaaaatgtca tcgtatatgg gattaccaac
    10861 aaatcagcac gatgctcttg agaatattca gctcttagta ggggctggtg cactactgta
    10921 tagcagcaat aaccctgagt taattgaact tgccagctcc atcctggctg taactcaaaa
    10981 atatagcctc accgcgtcac gtttatgcaa acaaaattta gctattaatt cccagaatcg
    11041 gattcgcacc caacgcgaaa cctgcggctt aacaaccgcc gaactcgcca ggctgctcga
    11101 actcgatgaa gaaatcatca tccagtggga gagcggagag tatgaaccaa ccatcagtat
    11161 gcttatccca ctggctaatg ttcttggctg cgatccgatg tggctgttaa ctggcgaggt
    11221 tactcctccg gagcaaccaa aaagtgagga gcagcaacac catgacgcat ctcaacaagt
    11281 ttgctcctta tctcgcgaag ctcttctacg gaagaaccaa taccaatggt gacataatcg
    11341 ccgcttcgcc cctcaaggta catgcgaaca tttttatcaa tcattgcgga aacagtctca
    11401 atatgaaaac acttctgaga ctcgctatat agcagaacat ataagtcagc tgaggaagcc
    11461 atgaaaaagt tcgaaaacat aactgttctc catgttgatg actttgatta tacaaacccg
    11521 gaacttctcc cggaggttgt aaaggcaata gatgttgccg atatagtgat tagaggaaag
    11581 agaattgtca aaaacaggct cgcatgcact tcaggagcaa tgacagaaac aacctcacaa
    11641 caagatgatt atgaaggcat ttgtctggag cctgattcat ttgcggtaaa tgtttatcat
    11701 ttattgcatg caacacaggt attacatatg tccagtcatc acgaaacaaa aacactcggc
    11761 agcgaaattc tgaattttac atgtgaatat gcaaaagctg ctgccgaaaa agaattagcg
    11821 caataacaac aaatatgccc taaaccttta ttacggtttt atcgccgggg attgttacaa
    11881 cctttattca cagatggaac tcagttatga ctttcctgaa aaataaggca tcgtataaaa
    11941 ctgcctgcct cattgcacaa catggagatt cttatcttca tatagccaac ctgtatttgc
    12001 gcaaagcata tgggagataa aataatggaa acatcagcac gaaataaaat acaggatgtg
    12061 cctgaggggg taatacacga aaaaataaga gtgttgctaa ccattgaaaa cgggaaagta
    12121 atttactcaa aacatctgct ggataatgaa ttcgttggct gcatggatac atttctgtgg
    12181 atggcaaaaa gagcgggtta cacgattatt ccaccagcaa aggagcaaac attatgaatc
    12241 attcagagtt ccgaccagaa gttacgccac atggcataaa aattggcaat acaaccattg
    12301 attatgttga ggccgtacag cggcttaatg atggtgaata cgattatcca aattctcaca
    12361 gtttaagaat tatgcaatgt attgctgaag ccgatgatgc cggattactg ggacgatttt
    12421 cagtcgatat gaagattgct caatggcgat ggctgtatgt gacgacgttt ataaatgaag
    12481 aagaaggcaa gaacggcacc attgatatcc ctaacgataa cggaactaca gatcgtgcag
    12541 ttatttataa ggggaagcat ggctgcctga gtatataccc aggaccactt cgcattgccc
    12601 tgcaaaacca tgtcgaatgg ggatttattg aaaaatatgg cgaagctgaa ggcatggggc
    12661 gagttctgtt tctctatcaa aaaatgctca tcgcagatcc tgataatggt ttcattgtct
    12721 ctgctatggg ccgcgaaggg cttgaactcc ttctggatga aatgattcac gatctgaata
    12781 ctcatggtat accagaagca ccagtgacac attaaattac acatggcaaa tcaaaataaa
    12841 attaatgtat ttcaggttga atcaaaacat aaaacacctg tgataaaaca cgttcgccgc
    12901 catacacgca tctatacgcc agaagagttt atggcaatgc caatgataaa gaaactcata
    12961 cgagacaatc ccggtctcgt tgctatagat aaaaacagtg gagaaataat gttatcacgt
    13021 gagattgccg aaatttactg tagcgtgaat aacagtaaaa agttgaaaaa ggcaatcaga
    13081 aagatatcag gaggagcatg aatatttatt tcagaatagt catatcactg gcaattatcg
    13141 catgcattta cggattactc gtcccgtttc tgatatcaat gaaggatacg gtagcggtaa
    13201 tttctggctt tgcactggcg tttctgaccc cgccatgcat ttatgccatt tataaggggc
    13261 tttctttcac taaggataaa agatgaaaaa aattattttt tctttagcca ttgttctgcc
    13321 agccattggc cttgtcggtt gtgatcgtgt tgagcctggt aatgtgggca tcaaagtcaa
    13381 caaacttggc gacgacaaag gcgtcggtga agtggtcggt gttggtcgct actggacagg
    13441 ctggaacact gaagtttata tcttcccgac ctttaagcag atgaagacct acgatgatcc
    13501 attcagtttc cagatgagcg atggaacaac cattggttac cgcatcggag tagcctacaa
    13561 ggttgatcca tccaaagtta ccacggtgtt tcaaacctac cgcaaaggtg tggatgacat
    13621 taccgacacc gacctgcgcc agaagatagc agatgcacta aatcggctgg ccagcaaaat
    13681 gaccactgat aaatttatcg acagtggcaa atctgaacta ctggatgcag ctctaaaaga
    13741 tattcaggca gaaatgacgc ccatcggtat tcaggtaatg agcctgtctt atgtaggtaa
    13801 accggagtac ccgccaaccg ttatcgacag cattaacgcc aaagtcacgg caaaccagaa
    13861 aacactgcag cgcgagcagg aagttaaaca gcgcgaagcg gaagccaaca tgttgcgcgc
    13921 ggaagctgcc ggacaggctg atgcgattcg cacaaaagct caggccgaag ccgatgctat
    13981 tcgtttacgc ggtgaagctc tgcgccagaa ccccggagtt atggagctgg aagccatcaa
    14041 taaatggaac ggcacactgc cgcaatacat gaccagcgat gccaatacac cctttatcca
    14101 ggttaaataa cttatatgcc cggcaggccg ccgggctaag ggaaaagcag atgaacactc
    14161 ataatatcca accgcaaata atgaactatg acccaaatct gacgttatgc ggacgcatgg
    14221 caaaacaaac cgttcgatta actttcgggc tatgggaata ccgcgaaaca ttcgaagtca
    14281 ctgtcggtgg caacctgacc ggactcgatg ttatcagttg cgctattgaa agcctgtacg
    14341 caacgctgcc ttatgaagaa gtcgaggatg agcgcgatat catagccacc attaatatcg
    14401 gcggactgga atgcaaggat gaaaagctgt ccggagagct ctggcttgcc gggatgctta
    14461 tctcagcaga aattatcagc attgaacccg ctacaaacat acgactctga agttcacact
    14521 attcagagag caggagaaaa aatgttcgct ttgattaatc agggacaact gtataccgac
    14581 agtgccggtt acccggtaaa aattattcgc tgcataaata acactgtgtt gtacagaaga
    14641 atggatgggc aaatgcagtc ggtaaaaata aacgatttta atggactgtt tgaacggatc
    14701 gatcacctgg aataccgaca aattctggca gaaacataac aggaagctca tctgaaaaaa
    14761 ttacgggcca tgaaaaggaa gtaaagaatg aataaagcat ttgaacaatg ggtccaccag
    14821 cgttacggca atcgctatga cctgacacga gatgttgacg gtttctactg tcgtgaaatt
    14881 gtgaaacgaa tgtttgaagt gtggtgccac tgccgtgggc tgagtgttgt gtgaggtaat
    14941 gcatgggcaa tgtgattcaa ctggctccca atgaatgggt ttgtgaaagc gttctaatcg
    15001 caattaccgg gctcaaacca ggcacaattc ttcgggcccg gaaagaatgc tggatggttg
    15061 gaagagagta tattcacgta tcaccagacg gtaatccaaa gccttccagt gaatgtatgt
    15121 ataacagaaa agcaatagat gcctgggtcg cttcaatgaa aaacaaacaa cctgggtgat
    15181 ttaatgccat gaagtatgta agctcgtatc gctcttgggc gtctggaggt atcaatggat
    15241 aaagtcaaat atccaacagg cgtcgaaaac cacggtggca cattacgcat ctggtttaat
    15301 tttaaaggta aacgtgtcag ggaaaatctt ggtgtccctg acactgccaa gaacaggaag
    15361 atcgccggag agctgcggac atctgtatgt tttgctattc gtacaggaaa ctttgattac
    15421 gctgcgcagt tccctgactc tcctaacctc aaggcttttg gggtaagtaa aaaagaaatt
    15481 acggtgaagg aacttgaaga aaagtggctg gatctgaaac gaatggaaat ctctgcaaat
    15541 gcattcaatc gctatgaatc cgttgcaaga acgatggttc cgaaaattgg aggcagcaga
    15601 ctggtgtcat cggtaactaa agaggaattg ctgtatatca ggaaagattt gctgaccgga
    15661 tatcagaatt caacgaaaaa caaagcagca gcaaaaggac ggagcgtcgt tactgtaaat
    15721 tattacatga cgacaatcgc tggaatgttt cagtttgctg cagatcacgg ttacttagaa
    15781 gcaaatcctt tccagggaat taagcctctt aaaagagcca gggcagagcc agatccgcta
    15841 actcgtgacg aatttattcg cctgatagat gcttgccgac atcagcagac gaaaaacctg
    15901 tggtcattgg ctgtgtacac aggaatgcgt cacggtgaac tggtctccct ggcctgggaa
    15961 gatatcgatc tgaaagcagg aactattact atcaggcgca attatacgaa actcggtgag
    16021 ttcactctac ctaaaactga agcaagtaca aacagggttg tgcatcttat ccagcccgct
    16081 atcagtgtcc tgaaaaatca ggctgaaatg acaagactgg gtaagcagca ccacatcaag
    16141 gttcaactac gtgaatatgg gcgttcagtg aatcatgaat gtactttcgt atttaacccc
    16201 caggtggtta gaaaaagcaa acaggtcggt tttatctaca aggtagattc tattggcgac
    16261 tcatgggaaa cagccattaa gcgtgcgggc atcaggcacc ggaaagcata ccagtcacga
    16321 cacacttatg cgtgctggtc attatctgcc ggagcaaacc caagcttcat tgccagccag
    16381 atgggccatg caagtgccct gatggtgttc aatgtatacg gagcatggat gactgacagt
    16441 aatgcagaac agatcgcaat gctgaatcag aagctggcag attatgtccc aatgatgtcc
    16501 catggtcacc aaagtgacac aagagactta ttaaaatcag tgggttagcc ttcaataccc
    16561 gtcatgttaa ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg
    16621 tcctgcgctg gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga
    16681 ccatttcgca tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc
    16741 gcatgtcacc gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg
    16801 gcccacggga gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt
    16861 atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaactctta
    16921 atcgcggttt acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg
    16981 cagcatgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg
    17041 tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga
    17101 cgcaaaaccg ggaattattt caggtctggt tacagaaatt agcgcagtgg catcagacca
    17161 ctacgcctta tcttttttta catacgccag acattgccca ggccccggaa ctggtacata
    17221 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
    17281 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
    17341 aagggagttt gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag
    17401 ttctcttttg atgtcgttcg cttgcgtttg cagtaccgcg ttgcctatgg tgacggtggt
    17461 tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc
    17521 gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
    17581 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
    17641 tttcgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
    17701 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
    17761 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
    17821 tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat
    17881 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
    17941 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
    18001 gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc
    18061 cccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt
    18121 agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
    18181 gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta
    18241 tcaaggactg aacccaggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc
    18301 caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag
    18361 cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt
    18421 ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca
    18481 gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt
    18541 ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc
    18601 gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac
    18661 gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc
    18721 gccgaaagcg aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg
    18781 aacctgatgc catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
    18841 tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc
    18901 accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc
    18961 gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg
    19021 cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt
    19081 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg
    19141 ccgctggagc atctctggca gttgaaagat caactggtga atgagggtga actggtgctg
    19201 gaaacgctgg ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg
    19261 caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag
    19321 tgtggttttg tagatatccg cgttgtggat gtgtgcgtta ccactactga agagcagcga
    19381 cgcactgaat ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt
    19441 aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa
    19501 aggtctggca ttactgccgg atgcggcttg aatgccttat ccggcctaca aacccttgct
    19561 aattcaatct attgcaggga c
//
LOCUS       DAHBHC010000090        19090 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHBHC010000090 DAHBHC010000000
VERSION     DAHBHC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.090"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..417)
                     /locus_tag="M2A35_004032"
     CDS             complement(<1..417)
                     /locus_tag="M2A35_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCB7200261.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKET"
     gene            complement(503..682)
                     /locus_tag="M2A35_004033"
     CDS             complement(503..682)
                     /locus_tag="M2A35_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCB7200262.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1063..1869)
                     /gene="trpA"
                     /locus_tag="M2A35_004034"
     CDS             complement(1063..1869)
                     /gene="trpA"
                     /locus_tag="M2A35_004034"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCB7200263.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFAMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFVCPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1869..3062)
                     /gene="trpB"
                     /locus_tag="M2A35_004035"
     CDS             complement(1869..3062)
                     /gene="trpB"
                     /locus_tag="M2A35_004035"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCB7200264.1"
                     /translation="MTTLLNSYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     AREEI"
     gene            complement(3074..4435)
                     /gene="trpCF"
                     /locus_tag="M2A35_004036"
     CDS             complement(3074..4435)
                     /gene="trpCF"
                     /locus_tag="M2A35_004036"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCB7200265.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(4436..6031)
                     /gene="trpD"
                     /locus_tag="M2A35_004037"
     CDS             complement(4436..6031)
                     /gene="trpD"
                     /locus_tag="M2A35_004037"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCB7200266.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6031..7593)
                     /gene="trpE"
                     /locus_tag="M2A35_004038"
     CDS             complement(6031..7593)
                     /gene="trpE"
                     /locus_tag="M2A35_004038"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCB7200267.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(7615..7708)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(7685..7729)
                     /gene="trpL"
                     /locus_tag="M2A35_004039"
     CDS             complement(7685..7729)
                     /gene="trpL"
                     /locus_tag="M2A35_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCB7200268.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            7867..8748
                     /gene="yciV"
                     /locus_tag="M2A35_004040"
     CDS             7867..8748
                     /gene="yciV"
                     /locus_tag="M2A35_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCB7200269.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            8745..9365
                     /locus_tag="M2A35_004041"
     CDS             8745..9365
                     /locus_tag="M2A35_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCB7200270.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            9466..10338
                     /gene="rluB"
                     /locus_tag="M2A35_004042"
     CDS             9466..10338
                     /gene="rluB"
                     /locus_tag="M2A35_004042"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCB7200271.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(10378..10968)
                     /gene="cobO"
                     /locus_tag="M2A35_004043"
     CDS             complement(10378..10968)
                     /gene="cobO"
                     /locus_tag="M2A35_004043"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCB7200272.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10965..11723)
                     /locus_tag="M2A35_004044"
     CDS             complement(10965..11723)
                     /locus_tag="M2A35_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCB7200273.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11943..12992
                     /gene="sohB"
                     /locus_tag="M2A35_004045"
     CDS             11943..12992
                     /gene="sohB"
                     /locus_tag="M2A35_004045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCB7200274.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13028..13279)
                     /locus_tag="M2A35_004046"
     CDS             complement(13028..13279)
                     /locus_tag="M2A35_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCB7200275.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13659..16256
                     /gene="topA"
                     /locus_tag="M2A35_004047"
     CDS             13659..16256
                     /gene="topA"
                     /locus_tag="M2A35_004047"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCB7200276.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGGALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16466..17440
                     /gene="cysB"
                     /locus_tag="M2A35_004048"
     CDS             16466..17440
                     /gene="cysB"
                     /locus_tag="M2A35_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCB7200277.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            17735..17899
                     /locus_tag="M2A35_004049"
     CDS             17735..17899
                     /locus_tag="M2A35_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCB7200278.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            17902..18069
                     /locus_tag="M2A35_004050"
     CDS             17902..18069
                     /locus_tag="M2A35_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200279.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            18183..18278
                     /locus_tag="M2A35_004051"
     CDS             18183..18278
                     /locus_tag="M2A35_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200280.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            18442..>19090
                     /locus_tag="M2A35_004052"
     CDS             18442..>19090
                     /locus_tag="M2A35_004052"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HCB7200281.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTT"
BASE COUNT         4676 a         4755 c         4971 g         4688 t
ORIGIN      
        1 ggtctctttc agaagcttcg ctgctttacg ataacctaat tgttcagcca gcgtcgctaa
       61 tgtcccgtaa ctggcaatct cataatgctc gactttctgt gctgcggcaa tcagtgcggc
      121 atcacgcact tcgtttttct cggtactttc gatgacctca ttagcttctt caataagacc
      181 ttccattgcc acacatttca tgcgcttaat tttcagattc gattctgatt ccacaacttg
      241 atcaatacgt tcaatctgtc catgtgtttc ctcgaggtgc gcatgaaaag cctgacttaa
      301 tttttcgttt gatgttgctc ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc
      361 gctgtaggta tctgaaagca ggtgaataaa tacatcttca atggtcttca tattcatatt
      421 tttctccagt gaaatcactg cgagccatac ggcccgcaga tgcaattatc tttatcagct
      481 gatattaata attaacggtg aatcaggatt tgcctgattt attaccaccg ctttgttgac
      541 cgcctttttt acccgcttca gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat
      601 gctgaccgcc tttacggcct gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg
      661 aaccaccacg atgttcggcc atgttatttc tcccgttgcg ttgcattgtt tcattaatat
      721 gagtattgtg tgtcgacact cattaaaatt agtcgctaat gagaattagt caaattaagc
      781 gcaacgagaa gatagaggga aaatatattt tgaggaacat tctggatata ttaacaatta
      841 cctgaggaat aagtgactta gagaaaatta ttgattttac tggtgttatg ttgcgggatt
      901 aatttgtttg attatattac tgttgggcgg aaaaatgacg taagttgacg ttcgaccggg
      961 gtaagcgaaa cggtaaaaag ataaatatta aatgaattta ggatttttcc ggcttcatta
     1021 aagaaagtta aaatgccgcc agcggaactg gcggctgtgg gattaactgc gcgtcgccgc
     1081 tttcatcggt tgtacaaaaa ctttcagtgc cgccagcatt ttctctggct cattaatatg
     1141 ttgctcgatg attttaacaa tggccgaacc agaaatcgcg cccgcagctc ctgcatcaat
     1201 cgctgctttt acctgatccg gggcggaaat accaaacccc tgcaatggag gtgcagcgtt
     1261 gtactctttc agcttcgcaa ccagatgatt gaggggtaac gcggcgcggt tttctgcgcc
     1321 ggtcacgccc gctcgtgaca gcaaataggt gtaaccacga ccgtaagagg ctatctggcg
     1381 cagcaggtcg tcatcggcat ttggcgggca gacgaagata ggtgcgacat tatggcgcaa
     1441 cgcggcctgg cggaagggcg cggactcttc aattggcaca tcggcaacca gcaccgaatc
     1501 gacgccgact ttctcgcact gggcataaaa ctcatcaatg cctttgttaa acaccagatt
     1561 ggcatacatc aacaggccaa tgggaatggt cgggtgtttc tggcgaatca gtgccagcat
     1621 tgcaaaacat tgtgccggag tcacacctgc cgcaaaggcg cgcagagtgg cgttttgaat
     1681 ggtcgggcca tccgctagtg ggtcggagaa ggggatacct aactccagcg cgtcagcacc
     1741 ggcttcaatt agcgtatcga taattttcaa tgactgctca atgcccggat caccgagcgt
     1801 gacgaaagga acgaatgcgc cttctttgcg ctccttcaac tgggcaaaca gagattcgta
     1861 gcgttccatc agatttcctc tcgtgctttc aaaatatcgt gaacggtgaa gatgtcttta
     1921 tcgccgcgac cggaaaggtt aaccaccagt agctgctctt tatccgggtt ttcgcgcatc
     1981 attttcaacg catgggccag ggcgtgggag gattccagcg ccgggatgat cccttcgtgc
     2041 aggcacagcg ttttgaaggc ttcaagggct tcatcatcgg taatagacac gtaatcagcg
     2101 cgtccagtgc tgttaagata cgcgtgttgt gggccgacag acgggaaatc cagtccggcg
     2161 gagatggagt aagattcttc aatctgcccg tcttcggttt gcatcatcgg cgctttcata
     2221 ccgaaataga tgcccacgcg accatgtttt aacggtgcgc cgtgctcgcc agtttcgata
     2281 ccgtgaccac ctggctccac accaatcagg ccgacgttgg tttcattgat gaaatcagca
     2341 aacatgccga tggcattcga accgccgcca acacaggcga taacggcatc cggcaggcga
     2401 ccttctcttt ccagaatctg cgctttggtt tcttcgccaa tcatccgctg aaactcacgc
     2461 acaatggtcg gataaggatg cgggccagct gcggtgccca gcatatagtg cgcggtttcg
     2521 taactaccgg accagtcgcg cagcgcctcg ttacaggcat ctttcagcgt cgcggaaccg
     2581 ctatgcaccg ggatcacttc cgcacccatt aagcgcatac gaaaaacgtt aggcgactgg
     2641 cgttctacgt ctttggcacc catataaata cggcatttca ggccgagcag ggcgctggca
     2701 agggccgacg ccacgccatg ctgaccggca ccggtttcgg cgatgatttc ggttttaccc
     2761 atccgcttcg ccagtaaagc ctgaccgagc acctggttag ttttatgcgc gccgccgtgc
     2821 agcaaatctt cacgcttgag atacagagtg gtgttcgtcc cggctgtaat gttctggcat
     2881 ttggtcagcg cggttggacg cccggcatag tttttcagca ggtcgttgaa ctgagcctga
     2941 aattcaggat ctttttgcgc actgacaaaa gcttcttcca gctggcgcag agcaggcatc
     3001 aggatttgtg gcacgtacat gccgccaaac tcaccaaaat aggagttaag taatgttgtc
     3061 attgttcctt tccttaatat gcgcgcagcg tctggaaaac cgaggccaaa agacgtgcgt
     3121 ctttgatgcc cggttgcgac tctacagcag aattaaaatc aagtccggcg cagccggttt
     3181 gtgccgcttc cacgcagtta tctgcgccta agccccccgc cagcagaacg ttgccaagcg
     3241 attgaccatt taatagtgac cagtcgaaac gttgcccgct tccaccctgg ccgttgtcta
     3301 aaacatattt atcaacgtgc tgaagctcgc gggcgggcag ggtttcaccg acgcttaatg
     3361 ctttccagat ggcaacgtgt gctggcagag cttcacgcag cgtatcgata tacagctgat
     3421 cttcattacc atgcagttgc actgccgcca gcgataacac cttagctttg tccaccacat
     3481 cggcaatatc gtgattgcgg aacacgccaa catactgcaa cggtgccgca gccatcactt
     3541 cctgcgcctg ttcaacgttg acgcaacgcg gtgatgtcgc aacaaaaatc aacccaccgt
     3601 aaatcgcgcc cgcgtcataa gctgctttag catcttgccc acgcgtcagg ccacatactt
     3661 tattctcacc cagcaacacc cggcgcacgg cggcgtgcaa atcgtcatgg gccatcaacg
     3721 ccgaaccaat cagaaaaccg ttagcgaagt ggcttaactc gcgcacctga gcgtaagtat
     3781 tgatgccgga ttcgctgatt accgttacgt tgtgccccag tttcggcgca agctcgcggg
     3841 tacggttgag atcaatcgac aaatcacgca gatcgcggtt gttgatgcca acgacctttg
     3901 cccccaatgc aatggcacgc tccagttcct cttcattact gacttcggtc agcacaccca
     3961 tctccagact gtgggcgacg gcggcaagct ggcgatattg ttcgtcatcc agtactgaaa
     4021 gcattaataa gcaggcatca gcctggtaat agcgcgccag atagatctgg taaggatcga
     4081 taatgaagtc tttacataaa atcggctgcg gggcgatttg gctgacgatg gggaggaaat
     4141 caaagctccc ctgaaaatat ttctcatcag tcagcaccga aattgccgaa gcgtaatgtt
     4201 tataaatggc ggcaatgcgt gctggatcga aatcatcacg gatcacgcct tttgacggcg
     4261 acgctttctt gcactccaga ataaacgccg tgcgcgcacc ctgtagcgca tcataaaaat
     4321 gtcgcgtgct cggctgaacc tcattctgaa aactggccag cggttgctgc tgtttgcggg
     4381 tttctaccca aatcgccttg tctgcgacga ttttcgctaa aacggtttgc atcatttacc
     4441 ctcgtgccgc cagtgcggta actctgtcgt aagcggaacc actgcgcagt acctcaagaa
     4501 cggtttgcgc attggcttgc agatcttcat ggccatgcag gcgcattaac atggcgacgt
     4561 tcgcagcgac ggctgcttca tgggcggcgt cgcctttacc ttgtaacaag cgtgttaaaa
     4621 tgtcacggtt ttcttccggt gttccgcctg ccagttgctc ctggtggtag ggagtcaggc
     4681 caaaatcttc agcggtcaat tgatagctct taatttcgcc gtcatgcagc tcggcaacga
     4741 ttgtcggcgc gtgtaatgaa acttcatcca tcccgccgct gtgcaccacc gccgcgcgtt
     4801 gataccccag cacgcgcaag gtttcggcaa tcggcagcac cagttccgga ctataaacac
     4861 caattaacgc cagcggcgga tgcgccgggt taatcaatgg ccccagcaca ttgaacaggg
     4921 tgcgggtttt cagttgctgg cgaaccggca tcgcgtggcg gaatccggtg tgatacttcg
     4981 gcgcaaagag gaaacataca cctaactcat ccagcgcctg gcgcgattta tcggcgttca
     5041 tatcaagatt aataccgaac gccgccagca gatcggacga accagattta ctggagacgc
     5101 tacggttgcc gtgtttcgcc actttcagcc cacaggccgc ggcgacaaac gcactggcgg
     5161 tagaaatatt gatactgttg ctgccgtcac cgccagtacc gacgatatca gcaaacagat
     5221 aatccgggcg cgggaacggc gcggcgtttt ccagtagcgc ggttgctgct ccggcgatct
     5281 cgttcgggtg ctcaccgcga attttcatgc tcaccagcgc cgccgccagt tgttccggct
     5341 tcagctcgcc acgcaccacc gctgaaaaca gctggtggct ttcttgttgg ctaagcgtct
     5401 gcgcctgata cagtttttcc agaatcggtt gcagcgtgtt ggctggctct agtttctgct
     5461 gcgcccaggc cagcgtttgt tccagcaggc gagcgccctg ggtggtgaga atggattccg
     5521 gatggaactg gaatccacaa acgcgatccg catcgtgacg tactgccatc accatgccat
     5581 taaaatgggc gttgatggtt aaaccggccg gaatgttact gccaaccagc gagtgataac
     5641 gcgccaccgg cagcgggttt gttaatccgg caaacatcgc ctgaccgtca tgttcaatgc
     5701 tcgacgcttt accgtgaaga atttcgcccg cctgaccgac atagcccccg taagcttcga
     5761 caattgcctg atgtccgagg caaatgccaa taatgggcag cttgccacgc aagcgggtga
     5821 ggagttccgg catacaaccg gcttcgctcg gcacaccggg gccaggagaa agcatcagta
     5881 ccggattgct catcgtcgcc aggcgttcaa ttaaggtctg cgccggaata tggttgcggt
     5941 aaatcaccac attatgccca ttgctgcgca actgatctgc caggttgtac gtaaaagagt
     6001 cgatattatc gagcagcaga atgtcagcca tcagaaagtc tcctgtgcat gatgcgcggt
     6061 ggcaatagcg cgcagtacag cgcgggcttt attacgggtt tcgtcggctt ccgactgcgg
     6121 aacagaatca aggactacgc cagcaccggc ttgcacggtg gcgataccgt tttccaccag
     6181 cgccgagcgg atcacaatgc aggtgtcgag atcgccatgc gcggtgaaat aacctaccgc
     6241 gccgccgtag ctgccgcggc ggcgaccttc cgcctcggca attaactgca tagcgcgtac
     6301 tttcggcgca ccacttaacg ttcccatatt catacaggcg cggtaagcgt gcagggcgtc
     6361 gagatcgtgg cgcagctcac cgaccacgcg ggagaccaag tgcatcacgt aagagtaacg
     6421 gtcaactttg gtgaggtcgg cgacgtagcg gctgccgggg gtgcaaatgc gtgccagatc
     6481 attacgggcg agatccacca gcatcagatg ttcagaaagc tctttatgat cggtacgcat
     6541 ttccagttcg atgcggctgt cgaggtctct gtccagcgag ccatcggcac gacgaccgcg
     6601 tggacgtgtt ccggcaatcg ggtagatctc aatctggcgg ctggtggcgt catacttgag
     6661 cgaactttcc ggcgacgcgc caaacagggt gaaatcatta tcctgcataa aaaacatgta
     6721 cgggctggga ttactctttt tcagcacgta ataggccgcc agcggtgacg ggcagggcag
     6781 agagaaacgg cgagatggca ccacctggaa aatttctcca gcgcgaatcg ctttttgcaa
     6841 caaacgcact acgccaccga actcttcatc gctctgatta cattcacaac gcatatgcgg
     6901 cacggaaacc actggcagcg gcggcgcggc ttcggtcagt tgctgacgta gttcgttcag
     6961 gcgagcagtg agacgttgtt tttcttcttc attcggagca aacaggctgg cctgaatacg
     7021 ggtgcttttt ttctgatggt caatcaccat cagagtttca gcgagataaa aacagaaatc
     7081 agggcagtta ttttccgctg acagttgcgg taaatcttca aatcccgcca caaggtcata
     7141 agagaacagg ccgccgaaga acattgcttc tcgttcttcc ttcggtacat tcaacagatt
     7201 ctgcaataaa cggaaagcgt caaaaaccga aagggagcat aagcgagcgt cttcatccag
     7261 cagtggactg acagggggga agcgcagcac acggcagttt ggtaattgtt cattttccac
     7321 acccgcaggc agggcgttat ccagtagtgt cagcagggct tcgccgttgc cggaaagtgc
     7381 ctggattgtg acagtgtcac ctaaagctgt aatgcgcagc gcactgtcta ccagcagcag
     7441 gctttttaaa tcatctttgc tgtcgatatc tgcggattcc agcagcagcg ttgccggacg
     7501 atccccacac aactggtgaa aaagcgcggt gggattgtcg cgataagcgc cttcgcaggt
     7561 tagcagttcg agagtcggtt tttgtgtttg cattgttatt ctctaatttt gttcaaaaaa
     7621 aagcccgctc attaggcggg ctgggtatct gattgcttta cgcatggtga atacactgcc
     7681 cgtttcagga agtgcgccac caacctttca gtacgaaaat tgctttcatt gtcgataccc
     7741 tttttacgtg aacttgcgta ctagttaact agttcgatga ttaattgtca acagctcatt
     7801 tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa aacattatcc agaacgggag
     7861 tgcgccttga gcgacacgaa ttatgcagtg atttacgacc tgcacagcca taccacagct
     7921 tccgatggct gcctgacgcc agaagcattg gtgcaccgtg cagtcgagat gcgcgtcggc
     7981 accctggcga tcaccgacca tgacaccaca gctgcaatcg cgccagcaag agaggaaatt
     8041 tcacgttcgg gactggcgtt gaatcttatt cccggcgtgg aaatttccac ggtctgggaa
     8101 aatcatgaaa ttcatattgt cgggctgaat attgatatta ctcatccgct aatgtgtgag
     8161 ttcctcgcgc agcagacaga acggcgcaat cagcgggcgc agctgattgc cgaaagactt
     8221 gaaaaagcgc aaatacctgg ggcgctggaa ggcgcacaac gactggcgca gggcggcgca
     8281 gtgacgcgcg gtcattttgc gcgtttcctt gttgagtgcg gcaaagccag ttcaatggcg
     8341 gatgtcttta aaaagtatct ggcgcgcggg aaaaccggat acgttccgcc acagtggtgt
     8401 acaatagaac aagctattga tgtcattcat cattctggcg gtaaggcggt gctagctcat
     8461 cctgggcggt acaatcttag tgctaaatgg ctgaaaagat tggttgcgca ttttgccgaa
     8521 caccacggtg acgcgatgga agtcgcgcag tgccagcaat cgcccaatga acgtacccag
     8581 ctggcggccc ttgcgcgtca gcatcattta tgggcatcac aaggatctga ttttcatcag
     8641 ccatgcccgt ggatcgaact gggtcgtaaa ctctggctgc ccgcaggcgt tgaaggcgtc
     8701 tggcagctat gggaacagcc gcagaacacc acagagaggg aattatgagc cagttttttt
     8761 atattcatcc tgataaccca cagcaacgtc tgatcaacca ggcggtggag atcgtgcgta
     8821 aaggcggggt gattgtttat ccaactgatt ccggctatgc gctcggctgt aaaattgaag
     8881 ataaaaacgc gatggagcgt atttgtcgta ttcgccagct gccggacggt cacaacttta
     8941 ccctgatgtg tcgcgatctt tctgaactct cgacctattc atttgttgat aacgttgcgt
     9001 ttcgtttaat gaaaaacaac acgccgggca actatacctt catcttgaag gggacgaagg
     9061 aagtgccacg ccgtctgttg caggaaaaac gcaaaaccat cggtatgcgt gtgccttcta
     9121 acccgattgc gcaggcgttg cttgaagcac tgggcgaacc gatgctttcc acttcgctaa
     9181 tgctgccagg cagcgaattt accgaatccg atcccgaaga aattaaagac cgtctggaaa
     9241 aacaggtgga tttgattatc cacggcggct atctcggaca gaaaccgaca acggttattg
     9301 atcttaccga tgatacgccg gtcgtggtgc gtgaaggcgt aggtgatgtg aagcctttct
     9361 tataagtgcg taactctgta tactacgcgg ccttaaaatg gcgctatacg gcgccattga
     9421 ttctcctgtt cgacgcctgt gaaggcgaca ccaaaaggaa gctctatgag cgaaaaatta
     9481 cagaaagtgc tggcccgcgc tggccacggc tcccgccgtg aaatcgaatc tattattgaa
     9541 gccggtcgtg tgagtgttga tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc
     9601 cctgggctga aaattcgtat cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa
     9661 atttgtcgcg ttctggccta ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg
     9721 gaagggcgtc cgacggtgtt tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc
     9781 gtaggtcgtc tggacgttaa cacctgtggt ctgctgctgt tcaccaccga tggtgaactg
     9841 gcaaaccgtt taatgcatcc aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt
     9901 ggtcaggttg acgacgcgaa actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt
     9961 ccggcagctt ttaaaaccat caagttcagc ggcggcgaag ggatcaacca gtggtacaac
    10021 gtgactctga ccgaaggccg taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg
    10081 caggtgagcc gcctgatccg tgttcgttac ggtgatatcc cactgccaaa aggtctgcca
    10141 cgcggtggct ggacggagct ggacctcgcc cagactaact atctgcgcga actggtggag
    10201 cttccgccag aaaccagctc taaagtcgct gtagaaaaag accgtcgtcg catgaaggct
    10261 aatcagattc gtcgtgcggt gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc
    10321 gggcgtaata acggttaatc aaagtatgcc ggacgtcata tccggcattt ttacagatta
    10381 ataatcaatc cctatttgcg ccttaacacc ggcatcaaac gcatgtttga tggggcgcag
    10441 ctcacttacc gtatccgcca gttcaagaat atcccgatga cagcctcgac ccgtgataat
    10501 caccgtctgt tgatgtggac gttcatttaa cgcctgcacc acttcttcca gtggcaaata
    10561 gtcatacgcc accatatacg tcagttcatc aagcaaaacc atatccagtg aggaatcagc
    10621 aagcatccgc tttgcatgtt gccagacttc gcggcaggcg gcggtatcag actcgcggtt
    10681 ttgtgtatcc caggtaaagc ccgttgccat cacctgaaac tcaacgccat gtggctccag
    10741 cagattgcgt tcgccattag gccaggtgcc tttaataaac tgcacgacgc ctactttttt
    10801 tccgtgacca actgcgcgtg ttgccgtacc aaatgccgcg gtggttttgc cttttccatt
    10861 gccggtaaag acgatgataa taccgcgttc atcctgggcc tgggccacac gagcatctac
    10921 tttttctttc actcgctgct gacgctgttg gtagcgttca tcactcattg ggaaattcct
    10981 ggtttacggc ccggttgggc gtcaaaggtc atgccggttt tacggcggct gtcatcgccc
    11041 atcagccaga ggtagagcgg catgatatca gcgggtgttt taagtttctg tggatcttcg
    11101 gtcgggaagg cgctggcacg cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg
    11161 cgcaggcgct gctgatattc atcggccagt acctgcatca tcccttcggt ggcaaatttc
    11221 gacgctgcat atgcgcccca gttggctcgt ccctgacgtc caacgcttga tgaagtaaag
    11281 accagtgaac cggcgtccga tttgagtaat aaaggaagca gtgcctgggt gagcataaag
    11341 gtggcattaa cgttgacctg tatgacgtcc tgccagacct gtggattttg ttcgctcatt
    11401 gggcaaacat cgccgagcaa tccggcatta tgcaaaacac catccagacg cggataatta
    11461 acggtaatgc gctgtgccag ttgttggcaa tcttcggacg tgcaggtcag caaatcgaga
    11521 ataaaccact gtggctgacg tccagtttct tcgtttatgt ggctggctac ctgacgtaat
    11581 ttttcttcat tacggcccaa cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg
    11641 gcttcacggc caatcccatc gctggctccc gtcaccagga taatgcgatc attgagtaaa
    11701 tcttgttttg gctggtaatg catggctact cctcaacgac gttgtctgtc gtgctgccag
    11761 tgtactttat gactttgggg ctttatgcct gaaacagaag ggcatttcaa tcagcctggg
    11821 gcgataacac cgcaaaatgg atactttgtc atactttcgc tgcaataaca tctctgcgag
    11881 acggcttaac atgcctgttg taaactgtga gccaaagcgt tgtttaacca aggtggggac
    11941 tcgtggaatt gttgtctgaa tatggtttgt ttttggcgaa aatcgttacc gttgtgctag
    12001 cgattgcggc gattgccgcc attattgtca atgttgctca acgtaataaa cgccagcgtg
    12061 gcgagttacg ggtcaacaat ctcagcgaac agtataagga gatgaaagaa gaactggccg
    12121 cggcgctgat ggactcacat cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca
    12181 agcaagaagc gaaagcagca aaagcgaaag ccaaactggg cgaggtggca actgacagta
    12241 aacctcgcgt ctgggtgctg gattttaaag gcagcatgga cgcccatgaa gtgaactcgc
    12301 tacgtgaaga gataacggcg gtactcgcag cattcaaacc gcaggatcag gttgtgctcc
    12361 gtctggaaag ccctggtggc atggtgcatg gttacgggtt ggcggcttcg cagctgcagc
    12421 gtctgcgtga taaaaacatt cctttaactg ttacggtaga caaagtcgct gccagcggcg
    12481 gttacatgat ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct attgtgggtt
    12541 ccattggggt ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc aaagatattg
    12601 atatcgaact gcacactgcc gggcagtata agcgtactct gacgttgctg ggtgaaaata
    12661 ccgaagaagg gcgggagaaa ttccgcgaag agttgaacga aacgcatcag ttgtttaaag
    12721 attttgtgaa gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg ggtgaacact
    12781 ggtacggaca acaggcggta gagaaaggcc tggttgatga aatcaacacc agtgatgaag
    12841 ttattcttag cctgatggaa ggccgtgaag tggttaatgt acgctatatg cagcgtaaac
    12901 gactcattga ccgattcacc ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct
    12961 ggtggcagcg gggtcaaaag ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg
    13021 ttttgcttta atcaaccaga tgatattttt ctgaaagcac atgggccagg tgtttgaaca
    13081 tattaaacac cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc
    13141 cggtaaatac cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg
    13201 tatcctcatg ctcacgaatg attttgttgg cctctttcaa cagcgtttcg cggtcgatgg
    13261 gttgtgtctc tttgttcatt atttactcct taaacaagga cattagtcta cgccaggcat
    13321 ggcttgcaga caaatatacc acgctggtgg caagagcgcc ttactggtaa ctttggattt
    13381 tgcatgctaa taaagttgcg tatcggattt tatcaggtac agtgtgacgc tttcgtcaat
    13441 ctggcaatag atttgcttga cattcgacca aaattccgtc gtgctatagc gcctgtaggc
    13501 caagacctgt taactcagtc acctgaattt tcgtgaacag agtcacgaca aggggttgat
    13561 atccgcagag aacgagtcca tatcggcaac tcgttgccag tggaaggttt atcaacgtgc
    13621 gacgcattcc tggaagaatc aaattaggta aggtgaatat gggtaaagct cttgtcatcg
    13681 ttgagtcccc ggcaaaagcc aaaacgatca acaagtatct gggtagtgac tacgtggtga
    13741 aatccagcgt cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg
    13801 ccgactctac ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg
    13861 tcaaccgtat gggggttgac ccgtggcaca attgggaggc gcactatgaa gtgttgcctg
    13921 gtaaagagaa ggtcgtctct gaactgaaac aactggctga aaaagccgac cacatctatc
    13981 tcgcaaccga ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg gaagtgattg
    14041 gtggtgatga tgcgcgctat agccgagtgg tgtttaacga aattactaaa aacgcgatcc
    14101 gccaggcatt taacaaaccg ggtgagctga atattgatcg tgttaatgcc cagcaggcgc
    14161 gtcgctttat ggaccgcgtg gttgggtata tggtttcgcc gctgctatgg aaaaagatcg
    14221 ctcgtggtct gtctgccggt cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg
    14281 agcgtgaaat taaagcgttc gtgccggaag agttctggga agtcgatgcc agcacgacca
    14341 cgccatctgg tggagcgttg gcgttgcagg tgactcatca gaacgacaaa ccgttccgtc
    14401 cggtcaacaa agaacaaact caggctgcgg taagtctgct ggaaaaagcg cgctacagcg
    14461 tgctggaacg tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt attacctcta
    14521 cgctgcaaca agctgccagc acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg
    14581 cgcagcgttt gtatgaagca ggctatatca cttacatgcg taccgactcc actaacctga
    14641 gtcaggacgc ggtaaatatg gttcgcggtt atatcagcga taattttggt aagaaatatc
    14701 tgccggaaag tccgaatcag tacgccagca aagaaaactc acaggaagcg catgaagcga
    14761 ttcgtccttc tgacgtcaat gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc
    14821 agaaactgta ccagttaatc tggcgtcagt tcgtagcctg ccagatgacc ccagcgaaat
    14881 atgactccac gacgctgacc gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta
    14941 ttttgcgttt tgatggctgg acaaaagtga tgcctgcgtt gcgtaaaggc gatgaagatc
    15001 gcatcttacc agcagttaat aaaggcgatg ctctgacgct cgttgaactt acaccagccc
    15061 agcactttac caagccgcca gcccgtttca gtgaagcatc gctggttaaa gagctggaaa
    15121 aacgtggtat cggtcgtccg tctacctatg cgtcgatcat ttcgaccatt caggatcgtg
    15181 gctacgtgcg agtagaaaat cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg
    15241 atcgccttga agaaaatttc cgcgagttaa tgaactacga ctttaccgcg cagatggaaa
    15301 acagcctcga ccaggtggca aatcacgaag cagagtggaa agctgtactg gatcacttct
    15361 tctcggattt cacccagcag ttagataaag ctgaaaaaga tccggaagag ggtggtatgc
    15421 gcccgaacca gatggttctg accagcattg actgcccgac ttgtggtcgc aaaatgggga
    15481 ttcgcacagc gagcaccggg gtattccttg gctgttctgg ctatgcgctg ccgccgaaag
    15541 agcgttgcaa aaccaccatt aacctggtgc cggaaaacga agtgctgaac gtgctggaag
    15601 gcgaagatgc tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg
    15661 cgatggacag ctatctcatc gatccgaaac gtaagttgca tgtctgtggt aataacccaa
    15721 cctgcgacgg ttacgagatc gaagagggcg aattccgcat taaaggttat gacggcccga
    15781 tcgttgagtg tgaaaaatgt ggctctgaaa tgcacctgaa aatggggcgt ttcggtaaat
    15841 acatggcctg caccaacgaa gagtgtaaaa acacacgtaa gattttacgt aacggcgaag
    15901 tggcaccacc gaaagaagat ccggtgccat tacctgagct gccgtgcgaa aaatcagatg
    15961 cttatttcgt gctgcgtgac ggtgctgccg gtgtgttcct ggctgccaac actttcccga
    16021 aatcgcgtga aacgcgtgcg ccactggtgg aagagcttta tcgcttccgc gaccgtctgc
    16081 cggaaaaact gcgttatctg gccgatgcgc cacagcagga tccggaaggt aataagacca
    16141 tggttcgctt tagccgtaaa accaaacagc aatatgtctc ttcggaaaaa gacggaaagg
    16201 cgactggctg gtcagcattt tatgttgatg gcaaatgggt tgaaggaaaa aaataacctt
    16261 taattctgtc aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat
    16321 tattttttct tcacacctat acactaaggc tataaatgat atagtggtta tagttagcac
    16381 cttttttatt attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg
    16441 atggcgtcta agtggatggt ttaacatgaa attacaacaa cttcgctata ttgttgaggt
    16501 ggtcaatcat aacctgaatg tctcatcaac agcggaagga ctttacacat cacaacccgg
    16561 gatcagtaaa caagtcagaa tgctggaaga cgagctaggc attcaaattt tttcccgaag
    16621 cggcaagcac ctgacgcagg taacgccagc aggacaagaa ataattcgta tcgctcgcga
    16681 agtcctgtcg aaagtcgatg ccataaaatc ggttgccggt gagcacacct ggccggataa
    16741 aggctcgctg tatatcgcca ccacgcatac ccaggcacgc tacgcgttac caaacgttat
    16801 caaaggcttt attgagcgtt atccccgcgt ttctttgcat atgcaccagg gctcgccgac
    16861 acaaattgct gatgccgtct ctaaaggcaa tgctgatttc gctatcgcca cagaagcgct
    16921 gcatctgtat gaagatttag tgatgttacc gtgctaccac tggaatcggg ctattgtagt
    16981 cactccggat cacccgctgg caggcaaaaa agccattacc attgaagaac tggcgcaata
    17041 tccgttggtg acatatacct tcggctttac cggacgttca gaactggata ctgcctttaa
    17101 ccgcgcaggg ttaacgccgc gtatcgtctt cacggcaacg gatgctgacg tcattaaaac
    17161 ttacgtccgg ttagggctgg gggtaggggt cattgccagc atggcggtgg atccggtcgc
    17221 cgatcccgac cttgtgcgtg ttgatgctca cgatatcttc agccacagta caaccaaaat
    17281 tggttttcgc cgtagtactt tcttgcgcag ttatatgtat gatttcattc agcgttttgc
    17341 accgcattta acgcgtgatg tcgttgatgc ggctgtcgca ttgcgctcta atgaagaaat
    17401 tgaggtcatg tttaaagata taaaactgcc ggaaaaataa tttcttgcgt tattttcggc
    17461 accttttatg tagcgaaggt gccggaatat attctctttt gttacttatt tctataccgt
    17521 gcacaatatt tttatccctc atatttatag ggtaaattac ctgtaaagta gtgtctaatt
    17581 acccgcctcg caaactccag ttttcaaata tctatttcag gtcaaaagat tgaattattc
    17641 actggagacg attcgtaaat tcgctggatt tttgactaaa gtttctttag gatttgtctc
    17701 atcgtatgat taattacacc aatcgttcaa cgtcttgctg ataagtgatg gcgattatat
    17761 gaggttagca atgccttctg gaaatcagga accccgccgc gatcctgaat taaaacgtaa
    17821 agcctggctg gcggtttttc ttggttctgc acttttctgg gtggttgtcg cactgctgat
    17881 ttggaaagtg tggggataac tatggtcggt caggagcaac tggagtcgtc accattatgc
    17941 cagcatagtg acaatgaaac agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac
    18001 tggcaactaa catcgcagca gcaagccttt atagaactgt ttgctgaaga tgatcagccg
    18061 aaacaataat tatcatcatt cttattaccc atttttaatg aattaaaggg cttttaatac
    18121 accgcagcaa taacagcttg agttatctca acacaaaata ataaccgtta agggtgtagc
    18181 ctatgatcaa cacaaatatg aaatattggt cctggatggg cgcgttttct ctgtcgatgc
    18241 tcttctgggc cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg
    18301 gttgtcattt gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat
    18361 cgttacgcga tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa
    18421 ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg
    18481 caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa atcactgggc
    18541 gatatcaccc gtctgcccaa gtcactcaaa gttttgctcg aaaacctgct gcgctggcag
    18601 gatggtaact cggttaccga agaggatatc cacgcgctgg caggatggct gaaaaatgcc
    18661 catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc
    18721 gtacctgccg ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat
    18781 actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc
    18841 gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac
    18901 cacgaacgtt atgtgttcct gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg
    18961 ccgccaggca caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg
    19021 agtgaattgc aggacggtga atggattgct tatccggata cactcgttgg tactgactcg
    19081 cacaccacta
//
LOCUS       DAHBHC010000091        18973 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHBHC010000091 DAHBHC010000000
VERSION     DAHBHC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.091"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            76..348
                     /locus_tag="M2A35_004053"
     CDS             76..348
                     /locus_tag="M2A35_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200282.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            329..1537
                     /locus_tag="M2A35_004054"
     CDS             329..1537
                     /locus_tag="M2A35_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCB7200283.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(1652..1864)
                     /locus_tag="M2A35_004055"
     CDS             complement(1652..1864)
                     /locus_tag="M2A35_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200284.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISWTYRKLKNQTIPLSTPYAT"
     gene            complement(2560..3591)
                     /locus_tag="M2A35_004056"
     CDS             complement(2560..3591)
                     /locus_tag="M2A35_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCB7200285.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSDETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            4564..4827
                     /locus_tag="M2A35_004057"
     CDS             4564..4827
                     /locus_tag="M2A35_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCB7200286.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            4745..5032
                     /locus_tag="M2A35_004058"
     CDS             4745..5032
                     /locus_tag="M2A35_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HCB7200287.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            5474..6007
                     /locus_tag="M2A35_004059"
     CDS             5474..6007
                     /locus_tag="M2A35_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCB7200288.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            6261..6944
                     /locus_tag="M2A35_004060"
     CDS             6261..6944
                     /locus_tag="M2A35_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HCB7200289.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            7116..7370
                     /locus_tag="M2A35_004061"
     CDS             7116..7370
                     /locus_tag="M2A35_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCB7200290.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            7546..7842
                     /locus_tag="M2A35_004062"
     CDS             7546..7842
                     /locus_tag="M2A35_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HCB7200291.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILASPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            8466..9533
                     /gene="pilL"
                     /locus_tag="M2A35_004063"
     CDS             8466..9533
                     /gene="pilL"
                     /locus_tag="M2A35_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HCB7200292.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWNNKTLTVTTSAAAPAQDTATTISPAAMVTTSTYPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            9533..9970
                     /gene="pilM"
                     /locus_tag="M2A35_004064"
     CDS             9533..9970
                     /gene="pilM"
                     /locus_tag="M2A35_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCB7200293.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            9984..11666
                     /locus_tag="M2A35_004065"
     CDS             9984..11666
                     /locus_tag="M2A35_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCB7200294.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            11659..12954
                     /gene="pilO2"
                     /locus_tag="M2A35_004066"
     CDS             11659..12954
                     /gene="pilO2"
                     /locus_tag="M2A35_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCB7200295.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            12941..13393
                     /gene="pilP"
                     /locus_tag="M2A35_004067"
     CDS             12941..13393
                     /gene="pilP"
                     /locus_tag="M2A35_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCB7200296.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPIIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            13404..14957
                     /gene="tadA"
                     /locus_tag="M2A35_004068"
     CDS             13404..14957
                     /gene="tadA"
                     /locus_tag="M2A35_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCB7200297.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            14970..16067
                     /locus_tag="M2A35_004069"
     CDS             14970..16067
                     /locus_tag="M2A35_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCB7200298.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            16085..16699
                     /locus_tag="M2A35_004070"
     CDS             16085..16699
                     /locus_tag="M2A35_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HCB7200299.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            16709..17269
                     /locus_tag="M2A35_004071"
     CDS             16709..17269
                     /locus_tag="M2A35_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCB7200300.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            17254..17910
                     /locus_tag="M2A35_004072"
     CDS             17254..17910
                     /locus_tag="M2A35_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCB7200301.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            17910..>18973
                     /gene="pilV"
                     /locus_tag="M2A35_004073"
     CDS             17910..>18973
                     /gene="pilV"
                     /locus_tag="M2A35_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCB7200302.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         5096 a         4573 c         4505 g         4799 t
ORIGIN      
        1 acggcagcct ggcggctgca aatcgtggtg tggcagaata tgagctttca gagtaaccat
       61 ccagaatgag ttcccatgtc acacaacctg gagcaccaga aagtccacac cagaatggtg
      121 aaagaggttc tgaaagccgt cgccagggcc aataatcacc cttatcagtc tgtatttaca
      181 gactttatcg ccggtcatcc ctcctgtacg gtctgtttct gggaaacctt ccacaaaatg
      241 tacccggatt caccgtacga atacgtcacg ttctgccata cctgccgtcg ttttgattta
      301 tacgaaactg aagcggagat gaaggcggat gacccgaagt ggtggtgatt ttcagccccg
      361 accgctgaaa cgcctgttca cagccaacca gtgctggaca tccttcctgg atgcgggcgg
      421 cctgcgcgat atcgaggttg aggctgtcac aaaaatgctg gcctgcggca cgcggatact
      481 gggtgtgaag gaatacaact gcgataaacc tgagtgcccc cacgtcagat acgtcactaa
      541 ctcatgtggc agccgtgcct gcccgtcctg cggaaaaaag gccacagacc tgtggatagc
      601 gacacagctg aatcgtcttc ctgactgcga ctgggtgcac ctggtcttca ccctgccgga
      661 cacgctgtgg ccggtgttcg aaagcaaccg ctggctgctg aatgacgtgt gccgtctggc
      721 ggtggagaat ctgctgtatg cagcccggaa acggggtctg gaacccggta tcttctgcgc
      781 catccatacg tatggccgtc gtctcaactg gcatccgcat gtgcatgtgt cagtgacctg
      841 cggaggtctg aataaacacg gacactggaa aaagctgagc ttcctgaaag acgcgatgcg
      901 ttcgcggtgg atgtggaata tgcggcagct gcttctgaaa gcgtggtcag aaggtctggc
      961 gatgccggaa tcgttgtcac atatcacgac ggaatcacag tggagaagcc tggtgctgaa
     1021 agccggcgga aaatactggc atgtgtacat gtcgaagaag acagccggag ggcggaatac
     1081 ggcacgctac ctgggtcgtt atctgaagaa gccaccaata gcggcttccc ggctggcgca
     1141 ttacaacgga ggagcgagtc tgagcttccg ttacctggac cacaaaacgg gagaaacggc
     1201 gacggaaacg ctgacacagc gggagctggt cgcgaggctg aaacagcaca tcccggagaa
     1261 gttttttaag atggtgagat acttcgggtt ccttgccaac cgggtgtgtg gagagaagct
     1321 gccgcaggtg taccgtgctc tggggatgga taagccggaa ccagtggcga aagtgtgcta
     1381 tgcacaaatg gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg
     1441 ccggatggta taccgccggg ccattgcggg gctgaatgta tcagggctga agaaaaacgc
     1501 gcgggatatc agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa
     1561 tcggtgcagg tataaaaaac agcctgttac ggtataaaaa tcgaccatgc agagagagaa
     1621 accaccggag tggagggaaa agcagaaagg ctcacgtggc ataaggcgtg gacagcggta
     1681 tcgtctggtt tttcaatttc ctatacgtcc aggatatgcc gcgccagcgg catggaaggc
     1741 ggcacgctgt ggttacatgt gataccggag taaaaccgcc gaagcccggc gtcagccggt
     1801 actgattgat agatttcacc ttacccatcc ccagccctgc taagccatac ccgctttcag
     1861 ccatgagaca gtttctgtgc gcggtcggag tggtcccgac gagggtttac ccgaagtcgg
     1921 ggcgtatctc cgcgttagcg ggccgtcagg gccgcttacg agcgtgtact gagaacatcc
     1981 agccagaaga ctgacagcga tgaaaatgta gttacaacat tcataattaa aagcgatgtt
     2041 gttcccgccc tttgggcggg ggcggcctcg cttttcagtt atgaggggtg ggcacggtgc
     2101 tgcgtttgtg tggtgttttt attcctgatg tttttttgtt gtggtttcag aacggtgagg
     2161 ttcggggccc gcgcgtcagc gtgggaggtg cttttagatt ctgagcggcg cggccttggc
     2221 cgctgcaagc gaaggcctct ttgtatttgt tcacagtggt ctcagagata aagattttcc
     2281 ctgcggcgaa gcaacgcagg ggattaaata atggggttaa gactatattg caggtttgct
     2341 gtttcaggtt tgcagcttct taaagttctt tgtttgcgga tttatgtgga taactgaaac
     2401 aacgtggcca tgttctgatg actgatgcgt ggttttatgg gcagatatgc ccctctgagg
     2461 ggatttaagg tgattactgc ggattttggg ctgagcggaa ccagcgccga aagccccgga
     2521 ggggctggtg atgattcagt gctgagggaa tggtgctgtt tagtgcggtg gcggggcagc
     2581 cagcgtcagt tcaagctgac gcaactccct gatggccatt cgcttcagat ggtcatcgga
     2641 acaaaaatag gcttcatccg gaggcagacg cttcctgaga tactctgcca tctcatgaat
     2701 ctgctggtcg gcaggcagtt tcttcaggcg tctggcgcgc ttactggctg ctgctttcgc
     2761 acgacggtgt ttcagcgcat cctgactgcg ctgcagatac cagcgttttc tggccgcatg
     2821 tacggagata tcttcatcct cacgcagaac accttcccga atgagctgct ggcggatttc
     2881 actttcacgc agtcgtttct gctgctgttc gtgaagtttt accatgtcga cgcccagcat
     2941 ctgccagcct gccggtgtta tccagacgta gcgtggcaga cgctggcggg tttcacggtc
     3001 ccacagggtt tcatctgaaa cacccagcac accaaaacgt acctgttcgg ccagcaaacg
     3061 ggaaaggcgg gagaccgtca cttccagttc cggaataacc ttccccttgc tgtctttcgg
     3121 gctgatttct ctggccagac gggaaacact catgcctacg gtatgtgtgc ccgcatcact
     3181 gaagctgacc agaaccggcc agacggcatc gaggaggcgt tgtttttccg gacggaaagc
     3241 atatttgcga ccggcatacg ttcgtttctg cacgtaaaaa ggatgcagag acatgtgcat
     3301 gcgcaggctc tgctcgccgg tcaccgggtc ctttttcacc agacggttgt aggcgtgccc
     3361 gagctgcccg ctcagcgcct tatagcgctc aggacgatac caggacggat tttcgcattt
     3421 gggtttggtg gagtgctcac cacgacgacg cttcgtgcgc ttcctttccg gttcaaccag
     3481 gaaagtggtc gcccttcccg cttcatagtc ttcccagaaa cggatctgct cttcgggtgc
     3541 taactgactc cagtgtactg cgttatatgg agtattcttc agacctgcca caagctgtcg
     3601 cttatggcgg agcgattgtg ccgtgtattt atacactgca cagggttgaa tctctggtac
     3661 ggtttcatta tactttaccc gtattctgtg aggcccccac tatctttcgt cgttccgcca
     3721 agttcgtaag aaagatagtg ggggttttcc ttttatggct tccggaacat cttcagtctt
     3781 ctgacttact gtccgtacat gccatctctc ctgaatgcct tatggcgcat tctgccgtcc
     3841 ggctttcgcc gctttgctcc acagagtgcc accctctgca cagctaataa cagccgaaat
     3901 atacccgctt tacgttaatt tgaaaagcat tacaacgatc tcttctcttc cagtgcctcc
     3961 tcactgatac ctctaataag ttaatcttga cctgcgtcaa aaaaataaaa tctattttta
     4021 aaagcagatt tttctgcttt agatctttct tttggattaa aagatcgact tttgtcctac
     4081 tcacgatcat ttatcttaca accgatctac ccaacagaat aatattctgt tttaatgcaa
     4141 ttaaacgatc taaataacct ttaaaaaatc attttgtaga tcattcgatc gtaaatagaa
     4201 taattcttag atgataaaac catttgttac acaacccgcc aattgtgtaa caacagcctc
     4261 tacaagagca tatttgttca tatgttcgtc agaactgcgg attgtctgga cgagggtcag
     4321 acaccgtttc gtgcatatgg accagcatgt tcgaacgcca caaagcagaa gcatcatgcc
     4381 tggatcgctt cggcgcggag cgttaagttt tgacgctttt ttgtatgttt tttaagctgt
     4441 tagttgactc ggtgcgttat actaagtacc ctttactggt cacaggcaaa actgtgatcg
     4501 ggcgtaggaa cccgcgtagt catcgtcggc gcagaagtta atttcttcat ttggcgtcgt
     4561 tttttgccat accataaaga caggaacttg tatttcttgt cgtcatctat ggtagctcag
     4621 gcggggcagg cattagcctg gccggtttcc gatgacgccg gtattcctac ccccgtctgg
     4681 gctatcacca atgagcgtag gaactcggtg atagcgtttt tagttgtcat cggagacctt
     4741 tgctatgtca cctcaactga tacagaaact cccggccatc actctgttgg aaggcatgtt
     4801 tcccgagcta tcaacaaacc agcttaaagt ctgcgttttt tacgcaatgg gtgttcctta
     4861 cgacgccatt gctcagaact gtcggctttc tcccgaaacg gtacgaacct acctgaagcg
     4921 aagcctgaag aatttaaatc tggaaggata tgatgcactt cgctctgctg tgctgatgcg
     4981 aactttcgtt ttcatgatta gcaatacagc taaagaaaac gaaaaaatgt gaacaggtgt
     5041 ccccattaaa ggggacactt tcatggaaat tgcattttgc actttacagg cgatcccaaa
     5101 ttgataaaca taaacaccaa gcacaagtta ctttaataaa gcaaatcact tcagacggtg
     5161 tcccagttat ttgtatctgg ctcatgcccg atatatggcg accttactct gaactgatgt
     5221 gctgatgttt tatttcgcgt atcacaatac tgtgccactg tattgctgat attagtgctt
     5281 agccgttctt tatctgtgag ttcgccaaat caccgataca gaacgtacta gagtctgctg
     5341 gttatatgcc tgtcagaact gcagtttatc cagatatggg ctggacactg cttcagacat
     5401 atgatttagc atattcgcct gacatcgcaa aatcgtccat catggatcgg ttttgcgcgg
     5461 agcgttgaac ttcgtgaata tagaacacct gaataaccgg aactggtatc ttgcgcaata
     5521 taatacagcc ggcaaaaaca gagaatcttt attctcatgg ctgaatgagc aaaacgtcgt
     5581 accatggact ccattaataa caagaaagat aagacgggca gactccaggt gctgttaccg
     5641 tgaaagaata ttcgctattt ttccagggta cttttttatt ctggctaatt ttgacattca
     5701 accagtatcg gcattacgtc gccatagcgc cttcattgat ttcgtgaagt ttggcggtga
     5761 aataaaaccc gtcaataaag atattgttga tggattaatg aagatttacc cggaccctgt
     5821 gctaaatccg ggagccaggg aagagctgaa tgcggcttcc agcatatggc tgacaaaagc
     5881 acaatatcag tatctcctca gaatggaaaa tactctccag cctgagtcga ggatatccct
     5941 gctcctggaa ctggtatcaa atgccgaaca tcacggcttt atggagcgcc ttgtaaacat
     6001 cccatagcca gtatttcccg gccttcgggc cgggcttttt tcaattcaag agaatattct
     6061 ggagtgaagc ctgcacggag ccatccccac catggactcc ccctgtcttt tgatggaacc
     6121 gtgtatttct taatggcgaa agaaataaaa ggcttcaccc cagagtattg attcgtttta
     6181 tcaaaggccg gcccgtgcca tcacgtgtgg cggagagaac ggataccggt cttcttttca
     6241 tctcacagta ggacattcgc atgaccacga cagaacatcc cgtgcgccag ttgctctggt
     6301 gtgcactgaa tgccctgaaa acggcacagg aaaaccacaa tataaccagt gaaaccggat
     6361 tacggcatta cctgcttgaa tggctttctg gtgccagcag acatcctgag ttccgtagtc
     6421 tccctggcga aatcatggcg ttaaaagcat tgaccgaaaa agatcgcaat attcccatca
     6481 tcggaacgct gaatacgctc tttctcagct ctgccactgt cggggaatgt ccgttgtttc
     6541 gttttcgggc tgcactgggt aaattacaga aagcaggctg gcgcacatat gtctgcccat
     6601 ggcctgagag ggtgttcaat gaatccattg aacgggcctg ttccgggaaa cgtcatctgc
     6661 tacaactaag tcgcaccgaa gagtgttttc taccgacagg cgatatgagg gcaccagtta
     6721 cgttgcagct gatcactcca ctacaccgca aaactatgca gcttctggaa acggcagagt
     6781 taatattctc cgacgaagga tttcaggtgg tgagaggata cgaacatcaa ttcgggatcg
     6841 aacgcagaga aaccctcttt cacaccctat ttatcgggct gcccagcctg ccagaagatg
     6901 tctggggtgc aacacaggag cagacgacta tcacattaca ctgaggatgt gtaatccccg
     6961 gcataccccg tttccagtct tcgcagcctg taccgccctt cctgccgttt ccgacattct
     7021 ttctgtcact actcgcttgt gccgtttttt cgcaaccggt acgtgcagtt atgacggtac
     7081 agctgcttct tttcccgttc cggaaggagt ctttgatgcc acagcaacat cccggacgac
     7141 tgcaaatact ggtcgtcgac gcacactgca aacgcaggct gttttcaacg aaaacaccga
     7201 cagaccccga tgagcttgcc cgacgtttct gcacgccgga taactgtctc gtggtggtac
     7261 tacgcgacaa ccgatttctg ttccgactgg agcgtgctcc cggcagccac tgccgctggc
     7321 acaagggcag cagctcccgc catcagcatc tacaggactg gctgtcgtaa acacaacaaa
     7381 aacaaaagac ctcgtcaaaa caggtggggg ccgtgttctt tttagttaaa tttaccatcg
     7441 cgattgcgat tgtctgtgta caccttttaa cgattttaat ttagccgttt gttgccatct
     7501 ttattttctt tattacctct ctccctttta tcatggagat taacgatgtt taccatgttc
     7561 tttctagtta aattaaccat cgcgattgtg attgtctgtg gaatcctttc tccgatttta
     7621 gcttcaccac ataacaaact gtccacactt ttttggaaga taccaaacgc gttctggatt
     7681 ttcctgattt gcatatactt tgcaatcgcc atggcggtgt taatagaaag cagtgaacag
     7741 acacgcatcc gccgccaaca atatttccaa cagtacgaag tgccaaatga gacacataaa
     7801 aatgtggtga tagaaaacag tattaaaagt caactaatat gaaaaataat aacgtaccat
     7861 tatatctatg gttgctttgg tgtacgcttt gtctgtacat cactacccca tggcaatact
     7921 tacgttatcc atacactgac aacttatcat tgccttatat gttatatttc tgtgtggtgg
     7981 ttgtgatttg cattttccca ctacttttca gatgtgtact tcttattgta aggtctgcat
     8041 ctaaatacga cttcacattc tttgtgaaga agaaaaaggg aagcaagaaa tcaactatac
     8101 atcttagccc ctatgctgac acacgttgcc cgaagtcaat gaggaaacac tgggatgtat
     8161 tgctttctct gatatcaaaa gcaattaata atgatgaaat cattgtcatg cattcacatc
     8221 tctttactcc agcaagaata aataaactta taaagagact ggaatcttat gatttgagtt
     8281 tttcagttat tacataccca cggaaaacaa gtctgtacga aaaattatca attcctttta
     8341 tatatctctt atttcagtgg tctttcccgc atttgcattc ggacggaatg acagtcgtca
     8401 ttcgtcctct ccagccgcct cacaccaatt aacaaacacg tatatacgaa cacggagtct
     8461 ccgttatgaa aaaaaactgt ctgacactta tactgccgtc attattgtcc ggctgtgccg
     8521 gaatttccgg cagcaataac gtggcaccat cagatacgcc tgctctggtt tttgttgacg
     8581 gacaaatcag tgaaagtgct ggcgttctgg cacttacgca acaacgtctt tctccaccta
     8641 aaataactcc ccccccgaga gtcagcgtgc cagagacagt aaaaacagca cagcctgtta
     8701 gcgaatcccg accgttacct tcaccaacaa taagtcagaa tttctcccgg ttaatcatta
     8761 caggcaagcc tggtagcgcc cctgtaacac tatcgccgac ggcccgtaac cggaccgtca
     8821 gtcagtggat aaaaacatta ctaccggaag gatggaaatt cagacaagag aatgcagcca
     8881 cgcccaagct gaataccaaa ctggtttcat ggtctgccaa tgatcagtgg acccgttctc
     8941 tgaaccggtt gctggaagaa caacacctct ggggccatct ggactggaat aataaaacgt
     9001 tgaccgtcac aacatcagct gctgcaccgg cacaggacac ggcaaccact atctcgccag
     9061 cagccatggt aaccacatcc acttaccctg aatctccaac gacagcaaat agccaaaaca
     9121 aaccacgaaa tccattcagg ggaaacagcg tatcttcgtc aacgcccgcc gccaccccga
     9181 ctccaaccgg ctccacagtc aagtctatcc cgctcatgac cggaacgcct gttaaaccgg
     9241 tatctcaggg taaagagtgg cgtgcgccgg caggcaccac attaagagag aacattatca
     9301 agtgggcaga agaaacaaaa tgcgaaagca tggcatcaac gcactggatg gtcatatggc
     9361 cactttctgt aaccgattac cgtctggatg cgcctttaat gttccgggga tcatttgaat
     9421 cagccatgga gcaggtattt gagttatatc ggactgcgca aaaaccttta tacgcacagg
     9481 cttctcgcat gcagtgcatc attacagttt ctgatacaag ggatggtcgc taatgggatg
     9541 gctggtcatg gccgtcggcc tgaccattct gattatcact ggtagctacc agaatcagaa
     9601 gatgtctgaa accacaaacg cccagcaata tgcaagtgcg tctgtatggg caagtcagat
     9661 actaatgatt gctaacagaa tcaatgatat cagatacgta tcaggccagc aggacggagt
     9721 aatttcctca gataaactgg ctctccctgt cactccggac tcccgaatta aacatcagtt
     9781 acaacagggg cgtctgtggg tatggatgcc tgaacaacca ggtcttgttg aaactttgcg
     9841 ttcaaaaagt cgtggttctg cactaatagg gatcttccaa aacgggcaac tcacctggct
     9901 atcaggaact gcaaccggtc tgacaccccc ggcaggcatt actgctggtt ctgtcgtcta
     9961 cgttaactaa tctggatata ttcatgaaaa aatcacacca gcgttcaatg aagctggcgg
    10021 tgctcccctg catgatcgcc gtcgctcttt ccatctccgg atgtactttc agcgaaatca
    10081 acaaaatgca gaaaaaagca caggaagact cagcacatgc acgggaaaag gtatcagccc
    10141 tttcggcccg taaatcgcag gctcttacct ggctcgataa tcaatggata aaccctgttc
    10201 cggtcgctca ggtatcaaga gagaaaaaac aaacagctcc ggcctgctac atcacgcagg
    10261 caagaaaagg agagatcact ctgcaggaac tggggcaacg tattactgcc gtatgcggca
    10321 tccctgtgat catcacgcct gacgcagcca attcaactct tgaaggaggc gctacccgcc
    10381 agatgacagg aacactacca gcaccagatg aaaatgggcg tctaccgtta agcagcctgg
    10441 gcagcacaac aatgactacc tccactcagc cattaacgct gaataacctc atgtggcagg
    10501 gagatatcaa tggtcttctg gatctgatgg ccagccggag tggtctgtac tggcgcatgg
    10561 ataatggtcg gattgtattc tatctgactg aaaccagaac gtatccactt catatgctga
    10621 acaccaaaac cagcagcagt tccagtgtca gctctggctc aacaagcaca atgggggcaa
    10681 caggaggcca ggataactca gcatccggtg atgcaacgtc ctctcagagc acaaccgttg
    10741 gtcaggaata cgatctgtat gaagacatcc ggaaaactat tgaagcaatg ctgacaccag
    10801 aaaaaggccg ttactggtta tctgcatcga gctcaacgct gactgtcact gatactccag
    10861 ctgtccagga agccgtcgca cgatatgtgg acgaacaaaa cagtattatg aaccgccagg
    10921 tagccctgaa cgtacaggtt ctgagcgtca gcaataccag aaacgaacag ttcggtctgg
    10981 actggaacct tgtttataaa tcgctacatt ccgccggagc aacgttgaac aatgcaagcg
    11041 gagattttac aggcgctaca tctgcaggcg tatcaattct ggatacggca acagggaatg
    11101 ccgccaaatt cagcggttcc agtcttctga ttaaagcgct gagtgaacag ggcgatgtca
    11161 gtgttgtgac ttcacaagaa agcactgtca caaacctgac gccggtacct atccagatgg
    11221 cagatcagac ggtttacgtc gcccagtcag caacaacaac gactacggat gtaggagcaa
    11281 caacaacatt aacgccgggc atgatcacca ccggattcaa tatgaccctg ctgcctttaa
    11341 ttcagaaaac gggcaatctc cagttgcaga tgaattttaa tctgtcagat cccccaacaa
    11401 tccgtagctt tacgtcaaaa gacggaaaca gttacatcga aatgccgtat accaaactgc
    11461 gttcactgag ccagaaggtc aatctgaaag aagggcaatc acttgtcgtt actggtttcg
    11521 atcagaacaa tacgacgaca agtaaagccg gtacgtttac gccagcaaat ccattatttg
    11581 gtggttcaca aaccgggaaa aatgaacgca gcacgcttgt aatcatcatt accccgactt
    11641 tcccgtcagg aggcaacaat ggctgatgaa gatatcaacc cggttgtgct tctcgcagat
    11701 cctaaagtaa atcatcgcgt gtgggcagcc tgtcttaaat ggagtcctgt cgtaaaaaaa
    11761 cagcgagtcc cttctcatca gaaacacaag ccccatgtaa aatccagacg gcttacctct
    11821 ctgaaagtga ctgtaggcag cagaagttcc cgtgggaaga tcagccgcat taccggcaca
    11881 ggaatactgg ccaggccgga gcgcaatcat tatttttccc ttgctctggc tttctgctca
    11941 tgggtgagaa atggatacgg cgtcttccgg tacagcgata aggagctttt attccttgcc
    12001 tccattaatg gccagcctgc cgttatggct gatctgtcag gaaatgatgc tgatgttgct
    12061 caaaaggtca gtctttttct gacgatgaac gaagagcccc ccgaaaaatg gcaggtcgta
    12121 tctcctctgg aacatcctga taactgggaa agtattatta cccggttatc atcagcagat
    12181 ctccgccgtt gcaaacttac tgtcggcaat cgcagcaaat tcacactgcc tgccgtactg
    12241 tttttagtag cagccggcgc aggcactgta ttctggatga ctcagccaga gccggatgtc
    12301 gtaccgacgc ccgaggagat agctgcacgg gcaagattgc agtttaaaaa gcccgatcca
    12361 ccgccagaac tcccgcatcc atgggcatca caacctgtaa tcagtgattt tctgaaagcc
    12421 tgcgcggacc ttcgaaaacc atcaccggtc gcgctagaag gctggaaact taccggtgga
    12481 acatgtactc ccgagacatt cacactgatt tacgaacgac agccaggagg aactattgaa
    12541 ggctttctgg caagaagcaa agaaatcttt aatgttattc ctgactttaa ccttaaagat
    12601 ggtgcacgcc tggcttctgt aacccggcca ctgccttccc tgccccgcag ggatgaggca
    12661 gtccctaccc cctcagaaca actgatgcgg gtgttcacgt ggtttcagaa aaaacagtta
    12721 acgccggcca ttaatgaaat agccataccg gaaccattac caggtaacga tggtgaacca
    12781 gcccctgttc agaaatggaa agaatatcag ttcagtttat caacgcctgt aaatcctgat
    12841 gagttgttcc ctttatttca ggataccggc gttcgtctga gcaatatcca ttttgaatta
    12901 aacggcggta ctttcagtta ttcctcagaa ggtcatattt atgcgtccag gtaaattact
    12961 cattattcca tccatatttt tcttctccgg tttttcattt gcaacaacac aacctcttgt
    13021 cacaatagga gaacttgaag cacaacaaaa ccggaatatt cttttacagg caaaagttca
    13081 gggcgcacag ctacagaaac aactcgaaga atcagatgtt acttcatcat ctgaaactgt
    13141 ttcaggtttc tctggtgtga ctgcctcttt gccatcagtt tcagagcagc cgacatctaa
    13201 aaacagaaag gaattgccca tcattatgga aataaacggc aaagataaac gtcttaatgc
    13261 cgttcttcgc atggctgatg gacgtcagac aagcgtcacc actggcagcc agcttccagg
    13321 cacatccgtc acagtgaagt cgatctcatt gtctggcgtc acgttaagtg atggcaccac
    13381 ccttactttc tgagtaacgt gatatggata aatataatct gaaggatgct ctccttttta
    13441 tatccagggg agacacccat gaaattctta tagaaacaaa ccagcggaca aggcctgacg
    13501 tacaaagtaa tctccaggag ctgctaaaat tatatccgga tatcaaccca aaagtagtct
    13561 cgttgtcgga gttacaggag gcaggtcagg atgagaaaaa cagaggcccg aaagaacgaa
    13621 ttattcatct gaaagacctt gctgatgtca gtgaaagtga gaagaaagtt ctatcttatt
    13681 ttgaaactgc cagaaaactt ggtgcttcag atattcattt cttaatttca gagtctattt
    13741 tcaaagttcg catgagaata ttcggtgaac tgcagactgt agatgaagat caaccagcac
    13801 tgggctattc actttgcgca acagccattc tgtcaatggc tgatgtcact gaaaccagtt
    13861 tcttccctca aagggagcag gatgcgcgcc tgtccccaca attaatgcga aaaataggaa
    13921 tatttggtgc acgctacagt cacagaccta cgggagacgg tctgattgca gttatgcgcc
    13981 tgatacccga tgacggagat aaagttccca cgtttaaaca actgggcttt atcccggaac
    14041 aaatccggtt gctgaacata atgctgcgcc gcccggaagg aaaaatagtc ctgtcaggtc
    14101 caaccggttc cggtaaatcg acaacgttac gcagcgcctg ccgtgtatat cttgacgata
    14161 atcaggggcg acacctgcta acaatcgaag atccgctgga aggacagata ctgggagcaa
    14221 cacagactcc gattatctgc gacaaatccg acgaagatgc cgtcaaactg gcatggagtc
    14281 gggctatttc atcggcaatg cgacttgacc ctgatgccat catggaaggg gaaatgcgcg
    14341 atcttatctc gatgatgtca accacctacg cggcacagac cgggcacata gttctgacga
    14401 cattacatac aaactcagct ctgggtatac cggagcgaat gattacgatg ggtatgaatg
    14461 ccgatcttat ttgcgatgcg caattactga ttgggatgat cagccagcgc cttgtaccaa
    14521 ctctgtgtcc ctcctgccgt attccatggg aaaagcgagc acctgaactt agcgacgacg
    14581 agcgtgatta ccttgagcgg cactgcaata aagattcact ctgcagtaca gataatatct
    14641 ggttccgaaa tcatcacgga tgctctgaat gtaatcacga cgtaatcatt aacggcagaa
    14701 agcgcggtga aataggtaaa ggcctcaccg gcaggactgt aattgctgag gtgatagaac
    14761 ctgataaccg cttatttcag attctgaaaa cacgcggcaa agttgcagca cgtaaatact
    14821 ggctggaaaa catgaaaggc atcagtcgtg tagagcatct tcttcgccgt atcaatgaag
    14881 gtctggtaga ccctcttgaa gccgacagaa tcatccctct ggacgaagac gagcgcctca
    14941 gcatcgatga tgtctaacag gagaacaaca tgcgtgaaat gaatttcagt caacggctga
    15001 ggcgtttcat tgtcagaaaa acgttcagcg ccccctacag ggtgcagttt tacgaagccc
    15061 tgcgttttct gctggaaaat aaacagccat taaagaccgc actggaacag atgcgggatg
    15121 cctggacaga ctttggacga aaatggcatc cctttgctga actggccaca gactgcattg
    15181 agtctcttcg tgaaaacagt ggcgaaaact ctctggaata tactcttagt ttatgggttc
    15241 ctcaggaaga ggccgcagta atcagcgccg ggataagaag tggttcaatt gtggatgcac
    15301 tgcaattcgc caccactctt acagatgcaa aagaacaaat ccatcaggct atatggcaaa
    15361 tggctattta tccggtggga ttgttaatca tgatgacggg gacattatat gttctgaata
    15421 cagagctaat acctgaactg agcaaaataa gctcccccga ttcatggagt ggtgctcttg
    15481 gttttcttta tggattatct gtttttgtcg ataattacgg cgctatatgt gccgttcttt
    15541 ttgcagtgat cacaggttta atatcatggt ctctgccaaa ctggaaaagc cctgacagtg
    15601 ttcgtacttt tgccgacaag ataatgccat ggagtattta tcaggatata cagggagcaa
    15661 catttctcct gaatatggct gcacttctga aagccaaaat gacgacgctc aactcgctaa
    15721 acattcttca ggagtttgca tcaccatggt taagcacacg tcttgacagc attatttacc
    15781 gcgtccgcca gggggatcat cttggactgg ctttgcgtca gtgcggttat cagttcccat
    15841 ccagggaagc agccaacttc ctgtctttat tacagggaga tggcgcaaca gagctaatca
    15901 gcaattatgg tcaacgatgg cttagtcaaa cacttcaacg tgtaaagaaa cgggcaaacg
    15961 tgattcgttt aattatgctg atatttcttg tcatgtctct catgttactg gtttttgcca
    16021 ttatggatat tcagtctatt agtgacaaca gcatgggtaa cttttaaaca aaacgaggat
    16081 atttatgtta gtagaaaata ttaataccac tttaactgga aataataaga agaatgagcc
    16141 tcatgataaa ggatgggcaa ttttggagca gggaacaata gcgctcgttg ttctttttgt
    16201 tatagtcgtt gttctgggta gtttgtatgc gttgcgtaca agagccaatg tggcaactga
    16261 aaccgcaaat atacaaacaa ttatcacaag tgcccagagc ctgctaaaag gtagcgatgg
    16321 atatacattt accagcagtg ccaaaatgac aggtgccctc attcagatgg gtgtcattcc
    16381 ctctgggatg accgtacagg gcgacaaaac atctggtaca gcaacgctct ataacgcatg
    16441 gggcggtgct gtaactgttg ccccggcttc aacatcaggg ttcaacaacg gttttaccgt
    16501 tacctatgat aaagtcccac aggatgcctg tatccagatt gccacacgaa tcagtaaaac
    16561 tggtttaact aacggcatta ccctgaacag tactgctcac agtgatggaa aagtcactac
    16621 tgaagaagcc agtacgcaat gcaaggcaga taatggcagc actggtacaa acaaacttat
    16681 cttcaccatc aatggttaag gagttttaat gtcagggggt acagtacccc tcagacttat
    16741 ttcattaatc ctgctttttt ctcttattcc acccgcccga gcttcatacg tagctcccaa
    16801 acagatctgg accaacaagt gggacaattg ctttgccgct gccggcgcgc gctatcaaat
    16861 tgaaccatta ttattgaagg ctatttctgc cggggaatct tcgttgaggc cgggcgcaat
    16921 taatataaat aaggacaaaa aaacaggtaa agccagtagc acggattatg ggttgatgca
    16981 aataaactca acccatattc caaagctgat aaatatgggc gtaataaaaa agtctgagga
    17041 tttaattacc aaaccatgcc ttaatatcca tataggaagc tggatactgg caagacattt
    17101 tcagatatgt ggcgtcagct ggaattgcct tgggtcatac aatgccggat tccgtaagga
    17161 cagacatgaa acaagagagc aatatgccaa taaaatatgg agaatttatc gtgacatgaa
    17221 agggatatgt cttcccggac agggaggcag gcaatgcaga caatcgtagg cataagtcta
    17281 tcccctgttt atatactgcc attgatgttc acattttcca ttattggacg aactctgcta
    17341 ttcagagttc gttatttttt atctgatacg ggacatctct ggtataagac acaccctgct
    17401 gttctgtcag gaatatggct atacagtata gctgtggcgt taattatttt atcatcatca
    17461 ccactgctct accgtattca tgctgtcctt attctttcgt tcatactgca aatggcggta
    17521 acggatgcac tgacaggttt attaccagga acattcaccc gacgtttttt aattgcagga
    17581 atgctatcac aaatcacgac tgatatatgg tggttccgta caacagaatt tgccactgct
    17641 gcaattgtct tattctgcct tcataaatta gttaaccgcc atcgtcttaa tattggcact
    17701 ggagacctgt ggcttattgc aggtattacc gcatggtcag ggttatataa cgccatatgg
    17761 tgtgtgttgc tgggcacagg aggatttgtc ttatggcatt caacatggtg cataaaaggg
    17821 cataaagaag gccctctggg tccatggcta tgttttggcc atgtcctttt gctgctggat
    17881 aatctttatc aaccactatg ggtaatctga tgaaaaaata tgaccgaggc tgggcatcgc
    17941 tggaaactgg cgcggcttta ctgattgtta tgctcctgat tgcctgggga gcgggcatat
    18001 ggcaggacta tattcaaaca aaaggatggc aaactgaagc acgtcttgtc agcaactgga
    18061 ccagtgcggc ccgctcttat atagggaaaa actacacgac tctccagggc agtagtacca
    18121 caacaactcc tgccgttatc acgacaacca tgctgaaaaa taccggcttt ttgtccagcg
    18181 ggtttactga gacaaacagc gaggggcagc ggttacaggc atatgtggtt cgaaacgccc
    18241 aaaacccgga attactacag gcaatggttg tatccagtgg tggcacacct tatccagtga
    18301 aagcacttat ccagatggct aaggatatta ccactggtct tggtggatat atccaggacg
    18361 gcaaaacagc cacaggtgca ttacgttcct ggtcagtagc tttaagtaat tatggtgcca
    18421 aaagcggtaa cgggcatatt gccgtattgt tatcgacaga tgaacttagt ggtgcagctg
    18481 aggacactga tcgtctttac agattccagg tcaatggtcg ccctgactta aacaaaatgc
    18541 acacggccat tgatatggga tcaaataacc tgaataacgt tggggcagta aatgcccaga
    18601 caggtaattt cagcggcaat gtgaatggtg taaatggcac tttcagcggt caggtaaaag
    18661 gcaatagcgg aaactttgac gtaaatgtca ccgctggcgg tgatatcaga agtaataatg
    18721 gttggttaat tactcgtaac agtaaaggct ggctcaatga aactcacggt ggcggatttt
    18781 atatgtccga tggatcatgg gttcgaagtg taaacaacaa gggcatctat accggcggtc
    18841 aggtgaaagg cggtactgtt cgggctgatg gtcgccttta tactggtgaa tacttacaac
    18901 tggaaagaac tgccgttgct ggcgcatcat gttcgcctaa cggccttgta ggccgcgata
    18961 atacaggggc aat
//
LOCUS       DAHBHC010000092        18946 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHBHC010000092 DAHBHC010000000
VERSION     DAHBHC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.092"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..341
                     /locus_tag="M2A35_004074"
     CDS             <1..341
                     /locus_tag="M2A35_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCB7200303.1"
                     /translation="TDIYFVSTFEPSTKSVDLLTVETFAGTICKQTDSEMPKEWTINR
                     GLYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIV
                     CEIFIPVRPV"
     gene            complement(462..4715)
                     /gene="fdeC"
                     /locus_tag="M2A35_004075"
     CDS             complement(462..4715)
                     /gene="fdeC"
                     /locus_tag="M2A35_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HCB7200304.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            5831..5932
                     /locus_tag="M2A35_004076"
     CDS             5831..5932
                     /locus_tag="M2A35_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200305.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            6296..6559
                     /locus_tag="M2A35_004077"
     CDS             6296..6559
                     /locus_tag="M2A35_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCB7200306.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            6559..6699
                     /gene="ykgO"
                     /locus_tag="M2A35_004078"
     CDS             6559..6699
                     /gene="ykgO"
                     /locus_tag="M2A35_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCB7200307.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(6769..6959)
                     /locus_tag="M2A35_004079"
                     /pseudo
     CDS             complement(6769..6959)
                     /locus_tag="M2A35_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7783..8325
                     /gene="ecpR"
                     /locus_tag="M2A35_004080"
                     /gene_synonym="matA"
     CDS             7783..8325
                     /gene="ecpR"
                     /locus_tag="M2A35_004080"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCB7200308.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            8400..8987
                     /gene="ecpA"
                     /locus_tag="M2A35_004081"
                     /gene_synonym="matB"
     CDS             8400..8987
                     /gene="ecpA"
                     /locus_tag="M2A35_004081"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCB7200309.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            9048..9716
                     /gene="ecpB"
                     /locus_tag="M2A35_004082"
                     /gene_synonym="yagY"
     CDS             9048..9716
                     /gene="ecpB"
                     /locus_tag="M2A35_004082"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCB7200310.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            9742..12267
                     /gene="ecpC"
                     /locus_tag="M2A35_004083"
                     /gene_synonym="yagX"
     CDS             9742..12267
                     /gene="ecpC"
                     /locus_tag="M2A35_004083"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HCB7200311.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            12257..13900
                     /gene="ecpD"
                     /locus_tag="M2A35_004084"
     CDS             12257..13900
                     /gene="ecpD"
                     /locus_tag="M2A35_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCB7200312.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            13869..14579
                     /gene="ecpE"
                     /locus_tag="M2A35_004085"
     CDS             13869..14579
                     /gene="ecpE"
                     /locus_tag="M2A35_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HCB7200313.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            14892..15221
                     /locus_tag="M2A35_004086"
     CDS             14892..15221
                     /locus_tag="M2A35_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCB7200314.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(15469..16081)
                     /locus_tag="M2A35_004087"
                     /pseudo
     CDS             complement(15469..16081)
                     /locus_tag="M2A35_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
     gene            16499..17188
                     /gene="paoA"
                     /locus_tag="M2A35_004088"
     CDS             16499..17188
                     /gene="paoA"
                     /locus_tag="M2A35_004088"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HCB7200315.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            17185..18141
                     /gene="paoB"
                     /locus_tag="M2A35_004089"
     CDS             17185..18141
                     /gene="paoB"
                     /locus_tag="M2A35_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HCB7200316.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            18138..18946
                     /gene="paoC"
                     /locus_tag="M2A35_004090"
                     /pseudo
     CDS             18138..18946
                     /gene="paoC"
                     /locus_tag="M2A35_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
BASE COUNT         4864 a         4896 c         4558 g         4628 t
ORIGIN      
        1 agacggatat ctatttcgtc tccacgtttg aaccgtcaac aaaatcggtc gatttgctca
       61 cggttgaaac ttttgccggt acgatatgca aacaaaccga ttcagagatg ccgaaagagt
      121 ggacaattaa ccgaggactg tatgcctctt tccgctatga aggtgagtgg gagcactacc
      181 ctgaatgggc gcgaaacctc tatctgatgg aattacccgc cagggggtta gccagagtga
      241 acggcagtga tattgagcgg ttttattaca atgaaaattt cgtcgaaatt gatagcaata
      301 acattgtttg cgaaattttt atccccgttc gtccggttta gttttcgcta tatctaaatg
      361 aatttataac gagactactg tcaggaaatt caataaataa tggcgttgaa gatgtcaacg
      421 ccattattgt ttattagaat gttactttca tattcttaaa attatttcat cgcctcctcg
      481 tcctttgccg tatcggcatc caccgcggta atggtcaccg tcactggctt catttcgtac
      541 ccgccatagg tcagggcgtt aaatgtgaac gtatagacac caggcttgct ggtactaaat
      601 ttacgcgtga tttttccgct cacgaaatgg tcatttttcg acggcaggaa ctgataatcc
      661 ttttccgtca ccccttccgg cgtctgaatg tcgacccaca tattcccgcc aacggggttg
      721 tttgccgaca tcagtgtcac ctccagcttc gcagtcgatt tcccatccgc caccagagat
      781 tgcatatcag atgatgcggt gagtaccgcg tttttatcat ccgcaacgaa tttagattcg
      841 gcagttttcg ttgatacctg accgttggtc tgactcactt gcgccttgag tgtgtatgtc
      901 gccgcaacag tagtggtagc ggtgaaaata gcctgccctt gctcattcgt tgtcgcagtg
      961 ccattcggtt ccaggtttaa cgaagccggg ctggcagtca gcgtcacttc gctatctttc
     1021 accaaattgt tattggcatc agtcaccgta actttatacg tttgtttggc tttgccgtcc
     1081 gccagcgcat agttgcccat cacctcaata ccggagacgg ttgcggtagc cttatcggct
     1141 acaaacgtta tagtctgcgt atccgaaggc tggctattcc ccgccgatgc cgtgatctca
     1201 tacgttccgg cagtcactga aaccacctgc aactctgctt tcccctcacc gttggctttc
     1261 acccagacat tatcgcctgt aagcggcttg acaccccggg gcagattaaa ggtcaccagg
     1321 ctccctggaa cagggttacc atttttatcc tcaacggtcg ccgtcagcgt aaaggcatct
     1381 ttgccgtttg ccacttttgg cgcggcgcta tcaacctcca gactcgcctg accggtactg
     1441 aaatccgcct tgaatttcac cgtaaccgtc ttctgagcat tattcactga ggccgtgatg
     1501 ctgtgttccc cggcaaccgt tgacatcagc tcaatgtcgg ctttacctgc cgcatcggtt
     1561 gttaccgtat tccccgtctt gctggtcaca cctttcggca gagtcagcgt cacattttgt
     1621 ccctgcaacg ggttaccata ggtgtccaca acggtcaggg ttacctggtt agtcgattgt
     1681 ccattagcca gctggttatc aaccttcacc gtcatatcac gaatctcagc cttagatgcg
     1741 tcaccagcaa cattcagcac cagtggctga gcaacggcat tttggccgtt cactcgcggc
     1801 atcacagaca actggcccgc ggcagatccc agagtcaagg tcgccacgta gaccccatta
     1861 cttgtttctg tccagtctcc tgttgaaggt cgctctgtcc ccgtgctggc ggcaccgcta
     1921 aacactggtg catctggctt catcccggtg accgggttcc cgtacgcatc cttcacggtg
     1981 aaggtgagtt ccgtcgtcgt tttgacggtc ggagccttat tgtccgcaac aagcgtagag
     2041 ttcgctgatg acatctctcc ggcgatgacc gtcaactgcg cggcttcggt ggctgctggc
     2101 tggccgttga atagcggcat gacacgaagc tcgcccgtct ttccgcctgt agttaacgta
     2161 gcaacatagg aaccgtcacc tttttcggtc caactggaaa cggtcgcccc ttcagaggcg
     2221 gtccccgtca aacttgccga caacgaaaga ccactgatag cgttgccatg tgcatctttt
     2281 gcaataagcg tcacggttgt actttcgccg gcttttacgt gatcttctgc gacagagact
     2341 ttcgactggt ttgaagataa cgcatcagcc accacggtta cttttgccgc atttgctgcc
     2401 gcatcctgtc catttagctt cggcataact tctaattcac ccgccgtaga gccgagagta
     2461 atctgcgcag tccacgtccc atcaccatta tttgtccagc ccgatgccgt agaacctaca
     2521 gcagcggcac ccgctaatga cggcgcttcc ggcgtgaggc tggtcacagg gttgtcatag
     2581 gcatctttta ccgtccagat tgccgtaact gtacccccaa ccaccggttt atcaggattc
     2641 agggtgatgg acgaatgtgc tgcatcaagc ggcccggcaa caaacttcag cgtttgttgt
     2701 aatgtgccca gctgatactg ctcgctgaaa gcacgcacaa caacgtttcc ggcacgggtc
     2761 gaagaaaccg tagcgctgta aacgcctggt tttatctccg aaattgtacc aatggtgaca
     2821 ccattagtgt cttgcggaac aaatcgcaag cggctggcct ctcccgtcac cgggttacca
     2881 tcagtatcca ccgcagtcag cgtcagcgtg taggcttgct gaccatcagc aaccacgtca
     2941 cctgacggct cgttagcgct cagggtggag tttgccacat ccatcatcgt ggcccgcagt
     3001 tctgcagtga cggttttgct catgccatca acactaacag taatcgttgc ctcacctgac
     3061 tgcgttccgg tagtaaagac agactgatac acccctgctt cagtttcggt gaactcaccc
     3121 agtgttggct ttgcctgtga tttagtggcc ttcagggtac gagtcacaat atttccagcc
     3181 ggtttgaaag ttagttcagt cttgatctga tctttcatgc ccgtgactgg ctggccctcg
     3241 gcgtcgcgca gagacagcac cagcggtctt tgctcattac cgttagcaag catttgaata
     3301 cggctctgac cgtcaagcgt taacgccgtg cgatcggcac tcatacctgc tccggtaatg
     3361 accacctctg tctgcacgcg ttttgaggca ttgcctttgt tatcgtaggc aaccgcagaa
     3421 atcgcataat aattgtcttt gcctggacga taagccggga gcgttacttg ccactgacta
     3481 ccctgaccgg taattttgcc accttcagcc agtaatgacg gcgcttccca ctgcacattt
     3541 ttcagtccgt gagttgcttt gctgaccaca agccccaggg aaagtgtctg accgccctta
     3601 ccttcaatac gctcaggcag agcaatacgg atcacttcag atttgcggta ctcaagaacg
     3661 atgttgttat tacgctcaac caggtcatag cggctgcctg ccagcattcg acgctcgcga
     3721 atgctgtctg tatcgagttg tttctccaga ggttcgccaa tccgataatt aacttccagg
     3781 ccaaagcgag tgtcattctc accgctcttg ccctgcttat gcccggcgct cagtgtcaga
     3841 agaggcactg gcgtataggt cacctcggca gaaatagcat gcgggtcttt ctggcgctta
     3901 tctttaccaa acagcccgac ttcatcgcca taatactgtt catacatcag gcttgcgcca
     3961 agctgcggcc aggcaggtaa atagccctct gcgcggatat cccaaccatt cgccgggcgt
     4021 tcctgataat cctcaatatc cggcgatttt ttccagccag aagcccgaat ataaccattg
     4081 gcgctcagtt tcagataatc gcgccagtat tccgcaccaa caccaatgcg ggtatgacta
     4141 cgggataaat catgatcgat aaaagtattc acccccgcca tccagtcatt tcctgaaaaa
     4201 tgacgccagc caaaaccaat atttgactga gtacgatcgt ctgtacgatg tattgccccc
     4261 tgagtgaaca acatatttgt cggcgtatca taaatcggat aaagcatttc cagcgaagaa
     4321 tccttcagcg agaaatcttt atcgacattc agtttgacgc gcgctgtacc atatttcccg
     4381 agccactcct gtatttcctg gttagcttta gcggtggcca ttccggtaat aaaattacgt
     4441 gtcgcatcgc tatctggctg actgcttaaa aatgtcccgg catttgcggc aaacgacgcg
     4501 acatttttct ccacattatt atcagcagtt accgtggtat tttccatgct caaccgtggc
     4561 tgaaccgcat gctgcgcatg tgccgccatt actggggtaa aggtgacagc gagtggaaaa
     4621 agaacctgaa cagagatatt tgcccacgcc acgcagcggg ccagaactga ataacgaaat
     4681 agtggttgtt tatgacctgt tttataacgt gacattgaga taatttcctg atgaacgaat
     4741 taacgaacat aggcagacac aaataatggc gtctgcttgt cagaaaaacc ggagatttcc
     4801 cggtttcctt ttccgcagaa aaggaaatac ctttatgtaa gaaagtacac cgccacctaa
     4861 tatatccagt gcctgtagcc actcatcagg tggttgggcg cgggattata tttttattta
     4921 tgggtaaaaa tttagctttt tgtgcaagtc attaaataga tacctaagtt atatttctta
     4981 ctctgcggac agtgaataat tcattattca gagtgaataa taatgacctg tctttaattt
     5041 accacgattg gcaatacatc gaagtaggta tatacgccag gataaaccaa tatgtttttt
     5101 atggatattc attttcattc atcagactgt aaattgcgga gatatcgaac ttgcactacc
     5161 catcgcaaca ctcaaacaga tttatgaatt tcgttcataa cccctgacaa atcctgccgt
     5221 ctaatcgagc ttttcccttt acgttatgct tctcatgaac gataacacaa cttgttcatg
     5281 aattaaccat tccggataaa ctatgggatc aaattcaggc atcgaaagga actgactggt
     5341 ttccgttaat gaattaaata tctattactc tcccgagagt aaaactatca aaccaatgag
     5401 taaccgatac agcctcgcaa attctttcag ataattaacc cgtcactacg gcacgttctc
     5461 tcgtaaggcg tgcctgcagt gtaatccagt atgtatccgg catagttgac ggtttaatag
     5521 aactcaattc ctctcccagg tcaccaacaa cgatatcttg tataacaccc caggagatac
     5581 gacattatga tgagatgcga tatgctcctc agcgccaatc aacgcccgga ctgggccagt
     5641 cttgagatca gactcattcc cggaactgac ttcaggcgac atatatcaat gacgttggtg
     5701 caataaaaga tagccgatgt aatacatcca ttcaaatttg tgatacatgt caaaaaattg
     5761 gttgaccaaa ctcgttattt atataagggc acttacgaag tgcgctcttt tttaaagcga
     5821 ggaagtacca atgaaagaga ataaagtaca acaaatcagt cataaactga ttaatatcgt
     5881 tgtttttgtc gcaattgtag aatacgccta tttatttctc catttctatt aataacggca
     5941 ataaactgtt cacttcagtg atatttaaaa tatgcatcct ctcccttttt gtaagtaatt
     6001 attatatccg tgggagagga atacacgctg tcaggtaatc aatcatactg cgataaatca
     6061 tcggccagta aagtggagat aacctccatt gtcgaaaaat ccatactctc agcgaaacca
     6121 tcatcaatca ctcatccagg cgtttatggg agcgtcgcca acggctgcta acaatgccag
     6181 acttccccgt tggggaaatt ccacatacca aaaatgggca cagggattgg gtgttgaaat
     6241 gatccggatg agcatgtatc tttatggtta tgttataaca taacaggtaa aaatgatgaa
     6301 gcccaatatc catcctgagt atcgtactgt ggtgttccac gacaccagtg ttgatgagta
     6361 ctttaaaatc ggctcgacta tcaaaacaga ccgtgagatt gagctggatg gcgtaacgta
     6421 tccatacgtg acaattgatg tctcttcgaa atcgcacccg ttctatacag ggaagctgag
     6481 aacagtggca tcagaaggaa atgttgcacg gttcacccaa cgttttggtc gttttgttag
     6541 cacgaaaaag ggggcatgat gaaagtcctt aactctctgc gtaccgcaaa agaacgccat
     6601 ccagactgtc agattgtgaa gcgaaaagga cggctgtatg tgatttgtaa atctaatcca
     6661 cgttttaagg ccgttcaggg tcgtaagaaa aaacgttgat ttaaaattcg acgaattaac
     6721 gatatttgtc tgattaataa tcagaccgga ttaatgttgg tgtatttatt acaccagcat
     6781 taatttttct ggggatgtat cttcctgttc atttaaggtc aactgcctga cgtttctctc
     6841 cgaatattcc attatattaa tgttgacttg ttgaccagct tcgccctgta tgctggcatc
     6901 aaccctcttt tagactgaac acgccactca gtttcctccc tttgcgacgc agcctgcatt
     6961 tacactcaaa ctgttaagat gataaatgtg gtaaatctgt tggtactgac ataaaaacgt
     7021 ttacgccaca ggaacggcct gatccaccgg taaccccatc gccgacgttc gagtgccagt
     7081 tagagtaacg cgcacagatg actgaatgca gtgccctggc aaagaggcca tcgttcctgt
     7141 gacaactggc agtcttcgtt taacttcact taatttggct cttagggggc ttaccggaca
     7201 gatgacgtac ttatacctgt ttaatttttc atcacttatt gggatgaaca cccataacca
     7261 ttttgtgcgg catgttaatc cattaaaaca ccttactgat tggcaaatca tctttaatta
     7321 tgactcatga tagttttata ttctatttct tatcatttaa acttgttttt ttactagtcc
     7381 attacacaac acattaagac tattcctaac acctcagggc aaagttcctg gctaatataa
     7441 aatgcaagta agaattgaac gttatattgc caataacctt atgaaactga atgtcttttt
     7501 cttcttatca aaaaagcaat attttcagtt tttctaaata ttgacttaac cattgaattc
     7561 cttttctgtt cacatattga cactcatcag gaaaaaaaca taaatttaaa cctaatcgaa
     7621 ataattaaaa cttaatctcg tttaacctat attgatatgt gctacgtatc ttatttactt
     7681 ccgatttact aaagaaactg aatgtacctg taaaaattac aggtttggaa agtagtgaca
     7741 tggcaaaatg attacagcag ggactatgag gttaaaaacc atatggaatg tcaaaaccgt
     7801 tctgataaat acatctggtc tccccatgac gcctacttct ataaaggact atctgaactg
     7861 attgtggata tcgacagatt aatttatcta tcgctggaga aaatcagaaa agatttcgtg
     7921 tttatcaatc tcaatacgga ttctttaact gaatttataa accgtgataa tgagtggtta
     7981 tccgcggtaa aggggaaaca ggtcgtattg attgcggcca gaaagtcaga agccttagca
     8041 aattattggt attacaacag caatattagg ggcgtggtat acgctggact gagtcgtgat
     8101 attagaaaag aactggccta tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa
     8161 gataaaatca ctgaccgaga aatggaaatt atccgcatga cggctcaggg aatgctgcct
     8221 aaatcgattg ccagaattga aaattgtagt gtaaagacag tgtataccca tcggcgtaat
     8281 gcagaggcca agctgtactc aaaaatatat aagttggttc agtaaactcc aggcaagtta
     8341 gttttaaaaa atgactcact gggacatcac gtcctcaatt caactcggga agaaatgcaa
     8401 tgaaaaaaaa ggttctggca atagctctgg taacggtgtt taccggcatg ggtgtggcgc
     8461 aggctgctga cgtaacagct caggctgtag cgacctggtc ggcaacagcc aaaaaagaca
     8521 ccaccagtaa gctggttgtg acgccactcg gtagcctggc gttccagtat gccgaaggca
     8581 ttaaaggttt taactcacag aaaggtctat ttgacgtggc tatcgagggt gactcaacgg
     8641 ctaccgcctt taaactgacc tcacgtctta tcaccaacac cttaacccag ttggatacct
     8701 caggttctac actgaatgtg ggcgtggatt ataacggcgc ggcagtcgaa aaaactggcg
     8761 ataccgtgat gatcgatacc gccaacggcg tactgggcgg caaccttagc ccgctggcta
     8821 acggttacaa tgccagcaat cgtaccaccg cacaggatgg tttcactttc tccatcatca
     8881 gcggcaccac caatggtacc accgcagtaa ccgattacag cactctaccg gaaggcatct
     8941 ggagcggcga cgttagcgta cagttcgacg cgacctggac cagttaatct ctctgatgta
     9001 ccagcagggg tagccccccc cctgctttat tcctggacgg aattgttatg aaaaagcacc
     9061 ttctgcttct cgctctgctg ttatccggaa tatctccggc ccaggcgctg gatgtcggcg
     9121 atatatcatc gtttatgaac agtgacagca gcacgctgag caaaacgatc aaaaacagta
     9181 ccgacagtgg tcgcctcatc aatatccgtc tcgaacggct ctcttcaccg cttgacgacg
     9241 ggcaggttat cgcaatggac aagccggatg agttgctact cactcccgcc agcttgctgc
     9301 tacccgccca agccagcgaa gtgatccgct tcttctataa gggaccagca gatgaaaaag
     9361 agcgctacta ccgcattgtc tggtttgatc aggccctcag tgatgcgcag cgcgataatg
     9421 ccaaccgcag cgctgtggcc actgcttccg cccgcatcgg caccattctg gtcgtcgcac
     9481 cccgccaggc aaactaccac tttcagtacg ccaacggctc cctgacaaat acaggaaatg
     9541 cgacgctgcg gatcctcgct tacggcccct gcctgaaagc cgccaatggt aaggagtgta
     9601 aagagaatta ctacctgatg ccgggcaagt cgcgtcgttt tacccgcgtg gacacggcgg
     9661 ataacaaagg acgggttgca ctttggcagg gtgataagtt cattcccgtg aaatagatag
     9721 ctgtgcagat ggataacgac aatgccttta cgacggttct ccccaggact gaaagcccag
     9781 tttgccttcg gcatggtctt tttgttcgtt cagcccgatg ccagcgctgc tgacataagt
     9841 gcgcagcaaa taggtggggt gattattccg caggccttca gtcaggcgct ccaggacggc
     9901 atgagcgtcc cgctttatat tcatctcgcc ggtagccagg gtcgccagga cgatcagcga
     9961 atcggcagcg cttttatctg gttggatcat ggacagctac gcatccggaa aatacagctg
    10021 gaagagagtg aagataacgc cagtgtcagc gaacaaactc gacagcagct gatggctctg
    10081 gcgaacgccc cgttcaatga ggcccttacc atccccctga ctgacaacgc gcagctggat
    10141 ctcagcttgc gccaactgct gctgcagctg gtggtcaagc gcgaagcgct gggcactgta
    10201 ctacgctcac gtagcgaaga catcgggcag tccagtgtta acaccctcag cagtaatctg
    10261 agctataact tcggcatcta taacaaccag ttgcgtaacg gcgggagcaa cacatccagc
    10321 tatctgtcgc tgaataacgt tactgcactg cgcgaacatc atgtggtgct cgacggctcg
    10381 ctgtacggga tcggtagcgg tcaacaggac agtgaattat ataaagcgat gtatgaacgc
    10441 gattttgccg gtcaccgatt tgccggtgga atgctcgaca cctggaactt gcagtcctta
    10501 gggccgatga ccgccatttc agcagggaag atttacggcc tttcctgggg aaaccaggcc
    10561 agctccacca tcttcgacag cagccagtca gccacgccag tgatcgcctt tttaccggcg
    10621 gcgggcgaag tacatctcac ccgtgatggg cggctactaa gcgttcagaa cttcactatg
    10681 ggcaatcatg aagtggatac ccggggtcta ccgtacggga tttacgatgt ggaagttgag
    10741 gtgatcgtta acggtcgcgt gatcagcaaa cgcccccagc gggtcaataa gctgtttagc
    10801 cgggggcgcg gcgtcggtgc accactggcg tggcaggtat ggggcggtag ctttcatatg
    10861 gatcgctggt cggaaaacgg gaaaaagacg cgaccagcta aagagagttg gctagcaggt
    10921 gcctcgacct ccggctcatt gagtacgctt agctgggcgg caacgggata tggatacgat
    10981 aatcaggcgg tgggtgaaac ccgtctgacg ctgccgcttg ggggagcgat caacgttaac
    11041 ctgcaaaata tgctggccag tgacagctca tggagcaaca tcgccagcat cagcgccact
    11101 ctaccgggag gctttagttc gctgtgggtt aaccaggaaa aaacccgcat tggcaatcaa
    11161 ttgcgacgta gcgatgccga caaccgtgca atcggcggca cactcaacct gaactcactg
    11221 tggtcgaagc tgggcacatt cagcatcagc tacaatgatg accgccgtta caacagccat
    11281 tattacacgg cagattacta tcaaaatgtc tacagcggtc cctttggttc gcttggcctg
    11341 cgggccggta ttcagcgcta taacaacggc gacagcaacg ccaatacagg gaaatatatc
    11401 gctctcgatc tctcgctacc actgggcaac tggtttagcg cagggatgac tcatcaaaac
    11461 ggctacacca tggcaaacct gtcagcacgc aagcagtttg atgaagggac cattcgcact
    11521 gttggtgcca atctgtcacg agccatctcc ggcgataccg gtgatgacaa aaccctcagc
    11581 ggtggggcgt atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa cgtcaatagc
    11641 gcggcggacg gctacgtcaa taccaattta accgccaatg gcagcgtcgg ctggcagggt
    11701 aaaaacattg ctgccagcgg gcggactgat ggcaacgctg gggtgatatt caacaccggg
    11761 ctggaggacg acggtcagat cagcgccaaa atcaacgggc ggattttccc gcttaacggc
    11821 aagcgtaact atctcccgct ctctccctat ggaagatatg aggtggagtt acagaacagc
    11881 aaaaactcac tcgacagtta cgatatcgtc agcggccgca aaagtcatct gactctctat
    11941 ccaggcaatg tcgctgtcat tgagccagag gtgaagcaga tggttaccgt ctccggccgt
    12001 atccgtgcgg aagacggcac actgctggct aacgcacgga ttaacaacca tatcggccga
    12061 acccgaaccg atgaaaacgg cgagtttgtc atggatgtgg acaagaaata ccccactatc
    12121 gattttcgct acagtggcaa taaaacctgc gaagtggcac tggaactcaa ccaggcgcgc
    12181 ggtgccgtct gggtcggaga tgtggtctgc agcggcctct catcgtgggc ggcggtgacg
    12241 cagacaggag aagagaatga gagttaacct actgatagcg atgataatct ttgcgctaat
    12301 ctggcctgca accgcgctca gagctgcagt gagcaaaaca acctgggcgg atgcaccggc
    12361 acgcgagttt gtgtttgtcg aaaacaactc agacgacaac tttttcgtca ctcctggcgg
    12421 ggcgctggat ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat acaatggttc
    12481 aggaaccatc tatcagcaaa gcctcggcta cattgataac ggttacaaca ccggccttta
    12541 taccaactgg aagtttgata tgtggctgga aaattcacca gtttcatctc ctttaactgg
    12601 cttgcgctgc atcaactggt acgctgggtg taatatgacc accagtctta tcctgccgca
    12661 aaccaccgac gccagtggat tttatggcgc gacggtgacc agcggcggcg cgaagtggat
    12721 gcacggcatg ttgtcagacg cgttttacca gtatctgcaa caaatgcccg tcggcagcag
    12781 ctttacaatg accatcaatg cctgccagac ctctgtgaac tatgacgcca gcagcggcgc
    12841 acgctgtaag gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc
    12901 agcaaatcta cggttgataa atacccactc gctggcggaa gtatttatca acagcgacgg
    12961 agtaccgact ctgggcgaag ggaacgccga ctgccggacg caaaccatcg gcagccgttc
    13021 aggattaagt tgtaagatgg ttaactatac cctgcaaaca aacggactca gcaacacctc
    13081 aatccatata ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga
    13141 tatgcagttc agtctgaatg gcagttcatg gaaaccggtg agcaatactg cctattacta
    13201 caccttcaac gagatgaaga gcgcagactc gatctatgtt ttcttctcga gcaacttctt
    13261 taagcagatg gtgaacctcg ggatcagcga tatcaacacc aaagatctat tcaactttcg
    13321 ctttcagaac accacatcac cggagtctgg ctggtatgaa ttttctacct ccaacacgct
    13381 gattatcaaa ccccgtgatt tcagcatcag tattatctcc gatgaatata ctcagacacc
    13441 gtcgcgggag ggatatgttg gcagcggcga gtcggcactc gatttcggct atatcgtaac
    13501 caccagcggt aaaacagctg ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt
    13561 gattggcggg cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt
    13621 cccggcgacg ctttccttta ttacccgcaa cggagctaca aaaacctacg atgccgggtg
    13681 cgatgatagc tggcgtgata tgaccgatgc gctgtggctg accacaccgt ggactgatat
    13741 ctctggcgaa gtggggcaga tggataagac cacagtcaaa ttttcgattc caatggataa
    13801 cgccatttcg ctgcgtacgg tagatgataa cggctggttt ggcgaagtca gcgcttcagg
    13861 agaaattcat gttcaggcga cgtggcgtaa cattaactaa ggccctgctg acagcggtct
    13921 gtatgctggc gacacctttg acacaggcga tttcggtcgg caatctgaca ttttcgctgc
    13981 cgtccgagac tgactttgtc agcaaacgtg tagtgaataa caacaaaagc gcacggatat
    14041 accgtattgc catcagtgct attgatagcc cgggcagcag tgaattgcgc acccgaccgg
    14101 tggatggtga actgcttttc gccccccgcc agctagcgtt gcaggctggt gagagcgagt
    14161 attttaaatt ttactatcat gggccacggg ataaccgcga gcgctactac cgggtctcat
    14221 ttcgcgaggt ccccactcgt aaccagacaa gacgtagccc aaccggcggc gaggtcagca
    14281 cggagccggt ggtggtgatg gataccattc tggtagtacg accacgtcag gttcagttta
    14341 aatggtcctt cgacaaggtg acaggaacgg taagtaacac tggcaacaca tggtttaagc
    14401 tactgattaa gccagaatgt gattcgaccg aagaggaagg cgatgcctgg tatctacgtc
    14461 cgggagacgt ggttcatcag cctgagttac gtcagccggg gaatcattat ctggtctata
    14521 acgacaaatt cattaagatt agcgattcct gtccggctaa gcccccttcg gcggactaag
    14581 acttatccat cggtcgaggg aagaattcca cctcagagcc ccaaaaatcg tttaaatgat
    14641 gtggaagcga tcgtcaatat cacctgtaac gatgggcttt ctcatatgca caccatagtg
    14701 ttcaggagac acggaaaggt atcaggcagc ctgctaacga gtggtttaat cacgaattag
    14761 tacgtaaaat cggtaacggc tggaaatcat tcaatactca cactatcgaa agttccccag
    14821 ccaaccgcgg tacgttctta catacgatgt accgctgttc tctttacgat ttttagctgt
    14881 actggtgaat tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt
    14941 tccgcgtctc tttttactgg gccgaagccg acgatgctgg cggcactatt cccgccaggc
    15001 tcactgaaca aatatcccct tttcaccgat gcatgagcgg taccaatcag aaaaaccccc
    15061 gatgtattgc ccttgcagga accccgggca aaaatgcctg ctgcacgata tataaaaatc
    15121 gatcgtccac atgcagagaa tttgccatgt ctggtgagaa cggagtcgtc aatgaggctt
    15181 gcaatcgtgc aagggctaaa tacgggctga caccgttata aacatacaat aattaattgc
    15241 actgccccgc cagcgataat agcggggctt cgtttttcag gggtaacaaa acccgatact
    15301 tcttctattt gccagcaaca atgcctctct tctgtagcgt tcttcggatc tacatcatcc
    15361 tgaagtagcg cgatatactc actgactatc tgaatcagaa tataaaaagc aatgttttta
    15421 acctataaaa atggcgctgt atttgcgcca tttttatcat tcaatgcatt atctgtttga
    15481 gcctaaaggg atctcagggt ctggctcatg agtaattctg tttcgtaaat ctctgcgaat
    15541 aatttcaata gaccagaacc agactaaatg tccaaaaatt tcagaaacat tctcatacca
    15601 cgggagatca aacagaggtg gcgtcagtcc catgagaggg aatgaaatca tatgaacaaa
    15661 aagttgggct aaagcacctg ccagtaagcc ctgccagagt ttaatttttg gaaatacttc
    15721 agcgaccaca caataaccga cagcaaacac tatcgaaaag ataatgtgcg taacaccaac
    15781 ccagttaaag acatgcccgg caaaggtata aacagccgca ttgggatctg tcagccccaa
    15841 ccagtctcga agaaaaatat acggtggatt gagaaaatta cgcgagcaat caatttggcc
    15901 tgcagccctg attaatgatt ccgggccaca cgctgcatta aacatatcca ccgggctacg
    15961 tggcggcaat ggaacttcag ccccacttca cgaatgcgga aacaacgcca gcaatcagcc
    16021 caatgaatgc agcaagacca taacgtctgc ggttcggtgg agtttgttca aatatattca
    16081 tatctaccct gcttgtacca ttatgttata cacctcttca ggagtattca taaaacaagg
    16141 taaatgtaaa gaactgtatt gttttgtata acaagatggt ttcctaatcg ccaatgaata
    16201 taagctccat catttctccc tatttttata ttagaagtga cagagatttg cagggtgatg
    16261 cagagctgaa aatcacaggt ttccttattg gtttttgcat cgtacaacta aagcaataaa
    16321 ccagctccta tcatcatcaa catcccggcg aacatgaatt tattagccgg gctcatagct
    16381 gcattcgcgt tttaagaata atcctcctgc tgtcgccgac tatgcttaac atttaaaaaa
    16441 gcatcagcac tctcgcaacg cactcttatt ttccccttta gaataccgga ggcctggtat
    16501 gagcaaccaa ggcgaatacc ccgaagataa tcgggttggg aagcacgagc cgcacgattt
    16561 aagtttgacc cgtcgcgatc tgattaaagt gagcgccgca acagcggcga ccgccgtggt
    16621 ttatcctcat tctacgctgg cggcaagcgt tccggcagct acacccgcgc cagagataat
    16681 gcccctgaca ctgaaggtga acggcaaaac cgagcagctt gaggtggata cccgaaccac
    16741 gctactggac gctttgcgtg aaaatctgca tttgatcggt accaagaaag gttgcgatca
    16801 cggacagtgc ggagcctgta ccgtgctggt caatggtcgc aggcttaatg cctgcctgac
    16861 gcttgcagtc atgcatcagg gggccgagat caccaccatt gaaggcctgg gttcgccaga
    16921 taatcttcac cccatgcagg cggcctttat caagcatgat ggcttccagt gcggctactg
    16981 cacctccggg caaatttgct catcagtagc ggcgctaaaa gagattcagg acggcattcc
    17041 cagtcacgtc acggtcgatt tggtttccgc tccagaaaca actgccgatg agatccgtga
    17101 acgtatgagc ggcaacatct gtcgctgtgg tgcatacgct aacatccttg ccgccattga
    17161 agatgctgcg ggggagataa aatcatgaag gcgtttacct atgaacgagt gaacacccca
    17221 gccgaggcgg cacttagcgc tcagcgcgta cccggcgcaa aatttatcgc gggcgggacc
    17281 aatctgctgg acctgatgaa gctggaaatt gaaacgccca cccaccttat cgatgtgaac
    17341 ggcctcgggc tcgataagat tgaagtgacc gacgcgggtg ggctgcgcat cggcgcactg
    17401 gtacggaaca ccgacctggc ggctcacgag cgcgtgcgtc gtgattacgc ggtactctcc
    17461 cgcgccctgc tcgctggcgc atctggtcag ttacgtaatc aggcaaccac cgcaggtaat
    17521 ctgctccagc gcacgcgctg cccctatttt tacgacacca atcagccctg caataagcgc
    17581 ctgcccggga gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc ggtggtaggc
    17641 gtaagtgaag cctgcatagc cacccatccg agcgatatgg cggtcgcaat gcggttgctg
    17701 gatgcggtgg tggaaaccat cacgccggag ggaaagactc gcagtatcac actggctgat
    17761 ttttatcacc caccggggaa aacgccgcac attgaaaccg ccctgcttcc cggtgagctt
    17821 atcgttgcgg tgacgttacc tccaccgctc ggcggaaaac atatctaccg taaggtgcgc
    17881 gatcgcgcct cctacgcctt tgccctggta tcggtcgcgg cgattattca gcctgacggc
    17941 agcgggcgcg tcgcgctggg cggagtagca cataagccct ggcgcattga ggctgcggat
    18001 gctcagctat cccagggggc gcaggccgta tatgacgcgc tgttcgccag cgcccatccc
    18061 accgctgaaa atacctttaa actcctgttg gcgaagcgaa cgcttgcctc cgtactggct
    18121 gaagcgaggg cacaggcatg aaatttgata aacccgcagg ggaaaacccg atcgatcagc
    18181 tgaaggttgt cggtcgtccc catgaccgca tcgacggacc gctgaaaact accggccggc
    18241 acgctacgcc tacgaatggc atgaagaagc ccccaacgcc gcctatggct atatcgtcgg
    18301 ttccgccatt gccaaaggac gcctcaccgc ccttgatacg gacgccgcgc aaaaagcgcc
    18361 gggcgtactg gctgtcatta ccgccagtaa cgccggggca ctcggcaaag gcgacaaaaa
    18421 caccgccaga ctgttaggcg gccccactat tgagcactat catcaggcca ttgcgctggt
    18481 agtggccgag accttcgaac aggcgcgagc ggcggcctcg ctggtgcagg cacactatcg
    18541 ccgtaataaa ggagcttact ccctggcgga cgaaaaacag gccgtcaatc agccgccgga
    18601 agacacgccc gacaaaaacg tcggtgactt tgacggggct ttcacctccg ctgcggtgaa
    18661 gattgatgct acctacacga ccccggacca gagccatatg gcgatggagc cgcatgcctc
    18721 gatggccgtc tgggatggaa ataagcttac cctctggacc tcaaatcaga tgattgactg
    18781 gtgccgcacc gatctggcaa aaacgctgaa agttcccgtg gagaatgtgc gtattatctc
    18841 cccgtatatc ggcggagggt ttggcggcaa gctgttcctg agaagcgatg cgctgctggc
    18901 ggccctcgcc gcccgagcgg tgaaacgtcc ggttaaagtg atgctc
//
LOCUS       DAHBHC010000093        18876 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHBHC010000093 DAHBHC010000000
VERSION     DAHBHC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.093"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..493)
                     /gene="rimK"
                     /locus_tag="M2A35_004091"
     CDS             complement(<1..493)
                     /gene="rimK"
                     /locus_tag="M2A35_004091"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCB7200317.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVI"
     gene            complement(554..1276)
                     /gene="nfsA"
                     /locus_tag="M2A35_004092"
     CDS             complement(554..1276)
                     /gene="nfsA"
                     /locus_tag="M2A35_004092"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCB7200318.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRQ
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(1260..1547)
                     /locus_tag="M2A35_004093"
     CDS             complement(1260..1547)
                     /locus_tag="M2A35_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HCB7200319.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            1707..1964
                     /gene="grxA"
                     /locus_tag="M2A35_004094"
     CDS             1707..1964
                     /gene="grxA"
                     /locus_tag="M2A35_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCB7200320.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(1994..2371)
                     /locus_tag="M2A35_004095"
     CDS             complement(1994..2371)
                     /locus_tag="M2A35_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCB7200321.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            2641..4326
                     /locus_tag="M2A35_004096"
     CDS             2641..4326
                     /locus_tag="M2A35_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCB7200322.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(4501..5037)
                     /gene="rcdA"
                     /locus_tag="M2A35_004097"
     CDS             complement(4501..5037)
                     /gene="rcdA"
                     /locus_tag="M2A35_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCB7200323.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            5121..6329
                     /locus_tag="M2A35_004098"
     CDS             5121..6329
                     /locus_tag="M2A35_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7200324.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            6329..7144
                     /gene="ybjI"
                     /locus_tag="M2A35_004099"
     CDS             6329..7144
                     /gene="ybjI"
                     /locus_tag="M2A35_004099"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCB7200325.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            7230..7514
                     /locus_tag="M2A35_004100"
     CDS             7230..7514
                     /locus_tag="M2A35_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200326.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(7555..8787)
                     /gene="mdfA"
                     /locus_tag="M2A35_004101"
     CDS             complement(7555..8787)
                     /gene="mdfA"
                     /locus_tag="M2A35_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCB7200327.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            9072..9668
                     /gene="ybjG"
                     /locus_tag="M2A35_004102"
     CDS             9072..9668
                     /gene="ybjG"
                     /locus_tag="M2A35_004102"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCB7200328.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9726..10484
                     /gene="deoR"
                     /locus_tag="M2A35_004103"
     CDS             9726..10484
                     /gene="deoR"
                     /locus_tag="M2A35_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCB7200329.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(10531..11733)
                     /gene="dacC"
                     /locus_tag="M2A35_004104"
     CDS             complement(10531..11733)
                     /gene="dacC"
                     /locus_tag="M2A35_004104"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCB7200330.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            11980..12606
                     /gene="gstB"
                     /locus_tag="M2A35_004105"
     CDS             11980..12606
                     /gene="gstB"
                     /locus_tag="M2A35_004105"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCB7200331.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(12603..13718)
                     /gene="yliI"
                     /locus_tag="M2A35_004106"
     CDS             complement(12603..13718)
                     /gene="yliI"
                     /locus_tag="M2A35_004106"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCB7200332.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(13829..14212)
                     /gene="bssR"
                     /locus_tag="M2A35_004107"
     CDS             complement(13829..14212)
                     /gene="bssR"
                     /locus_tag="M2A35_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCB7200333.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            14425..15750
                     /gene="rimO"
                     /locus_tag="M2A35_004108"
     CDS             14425..15750
                     /gene="rimO"
                     /locus_tag="M2A35_004108"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCB7200334.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(15797..17125)
                     /locus_tag="M2A35_004109"
     CDS             complement(15797..17125)
                     /locus_tag="M2A35_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCB7200335.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(17133..18875)
                     /locus_tag="M2A35_004110"
     CDS             complement(17133..18875)
                     /locus_tag="M2A35_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCB7200336.1"
                     /translation="MNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYN
                     IIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLDASDKLFYLPFAFIIIVLLVVYFM
                     TTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIE
                     RSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIV
                     RKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIH
                     KSAICEPLQGENAWSCAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRH
                     AMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELG
                     YQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSL
                     CVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQ
                     FTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPM
                     PFSAWDKSGKLVKE"
BASE COUNT         4757 a         4766 c         4518 g         4835 t
ORIGIN      
        1 caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc
       61 cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
      121 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
      181 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
      241 tccctagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa
      301 tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga atagaagacg
      361 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
      421 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
      481 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
      541 cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agaataaatg
      601 ggcggctttc tttaatgatt gtttggcgga tatgatcgct ccaggtatcc cggcgattat
      661 tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc agtgcgcctt
      721 tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa
      781 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
      841 gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg atatataccc
      901 cacccaatcc cagcgattcc gctgcggtta atgcattctg cgccatcatt gccgtatcaa
      961 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
     1021 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
     1081 cgccggtcag cgtcaccagc tcttcacgta acgctttgtc agtaatgcga ataatgctac
     1141 tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacgct
     1201 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatgacca caaataagtt
     1261 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
     1321 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
     1381 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
     1441 ggcagcgaca gggtgtcgct taccgacagc agcgccaccg ccagcaacat cattccgata
     1501 aattccagta tcaacacgct tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
     1561 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
     1621 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
     1681 ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt ccgggttgcc
     1741 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgattttc
     1801 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
     1861 caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
     1921 ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct gacaagccct
     1981 cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta ctgataaata
     2041 agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat gccagctctt
     2101 gccagaatga gcgggtcggt gaaaaaaaca gccgcataat aagcattgaa cagggtgccg
     2161 ccagcattgc gccaaacaga ggtttcagga cttctctacg ctgtgaaaag aagctggcga
     2221 ctgctccagg aagaatgaaa aatagcaagc cgatttcagg atgcccggca gcccgaaaag
     2281 cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca aaacagcaga
     2341 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
     2401 cacatttttc accagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
     2461 taggccaacg caattcatat agccgttgat acctaatgtg attacactag taaaatatat
     2521 tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa
     2581 acattatcgt aaattaccat ttctttcaac agcttactag taaacaagaa gttagcctcc
     2641 gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt atttgtggtc
     2701 ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact gggtaattcc
     2761 attggcgttt tagtcgtatc actgttatta ggccaacaac atttcagcat taacaccgat
     2821 gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac
     2881 tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact ggtgatggtt
     2941 ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga tattggcctg
     3001 acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat
     3061 acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga taatctgagc
     3121 ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac
     3181 ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc ccgcgaacgt
     3241 ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc
     3301 ggcccggagc tggtggcctg gaccgacggc aaaaatctgc gtgaactggg tatttatcga
     3361 caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa tccagacggt
     3421 gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga cgcccatgcc
     3481 cgactcgatc ccagcttccg taacggcaaa gaagttttcg atcgtgacct tctcgacatg
     3541 cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ccgttggcaa acgtctcgca
     3601 caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag ccagattgag
     3661 atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt cagcggcgat
     3721 gcccgccgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca cagccaggtc
     3781 actgacttgc tggcattctg cgccttcttt gttattgggc tgatgatcgg gatgatcaca
     3841 ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt attcgccgga
     3901 attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc tcagggtgca
     3961 ttaagcatgg tgaaagagtt cggcttaatg gtgtttatgg caggcgttgg tctgagcgcc
     4021 ggtagcggta ttaataacgg tctgggcgcg attggcggtc agatgttgat tgccggattg
     4081 attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg
     4141 aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc agcaatggag
     4201 atcatcagtg atacagctcg cagtaacatc ccggcgctgg gctatgcggg cacctatgca
     4261 atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg gccaggatta
     4321 ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag aacttttccg
     4381 cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt ttgtggagcc
     4441 catcaacccc gccatttcgg ttcaaggttg gtgggttttt tgttatctaa aatttatcta
     4501 ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc
     4561 ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg cggttccggg
     4621 ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca ttaccgtttt
     4681 taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca tattatccgg
     4741 cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc ctggagcatc
     4801 actaacatcg ctaaaaaatg cctgatattg ccgggacatg atctcagtaa aactgctgaa
     4861 cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc ccaacggtac
     4921 cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc
     4981 ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc
     5041 atttcctctc tctgcaattg tgtacaaatg tacacaacct tgctaatgtt gtcttccctc
     5101 ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg aaacgccgaa
     5161 cctgggcgct gtttatgttc ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc
     5221 gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc ggtgttctct
     5281 ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta gtgaaacgct
     5341 ttgggacacg taatgtcatc ctggtcacca tgtcctgcgc attggtcggg atgatgatat
     5401 taagtctggc actctggctg acatcgcccc tgctctttgc cgttggtctc ggcgtctttg
     5461 gggcaagttt tggttctgcg gaagtggcga taaacgttga aggtgccgcc gttgagcgag
     5521 aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc acgctggcag
     5581 gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg cacattttat
     5641 tggcggcgct ggtaggcatc gcgcctatat atatcgccat tcaggcaatc cctgacggta
     5701 cgggcaaaaa tgctgccgat ggcacccagc atggcgaaaa aggcgtacct ttttatcgcg
     5761 atatccagtt gctgctgatt ggtgttgtgg tgctggcgat ggcctttgcc gaaggttctg
     5821 ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagcccc acttccggct
     5881 cgctgattta tgccggtttt accctgggga tgaccgttgg acgcttcact ggcggttggt
     5941 tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcgctaatg ggggcgttgg
     6001 gtattgggct gattattttt gtcgatagcg cctgggtcgc tggggtgtct gttgtactct
     6061 ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc gataccggcc
     6121 ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct ttcctcgtcg
     6181 ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca atgctggttg
     6241 tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca
     6301 aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg tagacatgga
     6361 tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg ctcagtatca
     6421 gcaaatgaaa gcacaaggaa ttcgctttgt agtcgccagc gggaatcaat attatcagtt
     6481 gatctctttc ttccctgaaa ttgctaatga aattgccttt gtggctgaaa acggcggctg
     6541 ggtagtgagc gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg cgtttactac
     6601 tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag
     6661 cgcctatacg ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa tgtattatca
     6721 ccgtctggaa tacgtcgata actttgacaa cttagaggat atcttcttta agtttggcct
     6781 gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg ccatcggcga
     6841 tattatggtg ccggtccaca ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca
     6901 taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca gcgaagtggt
     6961 ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct ttagttttgc
     7021 aatggaaaat gccggcaatg cggtcgtcgc agcggcaaaa taccgggcag gctccaataa
     7081 ccgtgaaggc gtactggatg tgatcgataa agtgcttaaa cacgaagcgc catttgacca
     7141 ataaaaacgg cagcacgccc ggcaacacaa tacctgaatg aaggctgcat aattcgcgga
     7201 aattaacccg ctgcattatt tcaggcgaaa tgtttatgaa aaattgtcta ctgttgggcg
     7261 cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc gatgtgccga
     7321 gtggatacaa agtggttgtg gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg
     7381 cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac cgcccgcatc
     7441 cttatgttcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac cagattgacg
     7501 accatcacca ttaattcgag aatgcctgat cgcacaaagc aatcaggcat ttttttaccc
     7561 ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg acagccacaa
     7621 aattccgttg acaaggttga agagattaaa cagtccgttg cccccgttca gccaggcatg
     7681 tttgctgatt tcaataccga cggtaaagat cagcatttgc agcattccca tcgccgcaga
     7741 aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc
     7801 cagaccaatg ccgaaagcat aaatacttaa cccggcggtc atccataaat acgcgtgcga
     7861 tgagataacc gttgccgcag cagcgaccaa cagaccaata ataatcggcc agccgcccat
     7921 aataatcagc gaacgtacgg tgcggcgcga ggtcagacgc gctaacagca agttacccgc
     7981 aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc tcaactgctc
     8041 gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca ggctaacgaa
     8101 tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct tatagtcacg
     8161 accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag gcatggcttg
     8221 ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aagacaaaca tcccttccca
     8281 gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag caatcagcgc
     8341 cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg attcctgaat
     8401 tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga agcgcaacag
     8461 agtgaattgt tcaatgtttt gcgccagtaa tattgccaga caggtgatga taaaccacac
     8521 cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc ccaggagcca
     8581 ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc aatcaatgcc
     8641 cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt tgccgatata
     8701 ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac gtccaagcct
     8761 ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat cgcgcagagt
     8821 ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg gttaagcaca
     8881 ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac ctatgcccgt
     8941 tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg ctttacccag
     9001 tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta atcactttgc
     9061 gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt aacgcgacgc
     9121 cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat ttgattaccg
     9181 tggtaccgct gctggccgcg gtgctttggt tgtgggggct tacagcacaa cggcaactgg
     9241 tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg atgggacatc
     9301 tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg catcatgcgg
     9361 cggatgactc attcccaagc gaccacggta cggtgatttt cacctttgca ctggcatttt
     9421 tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc gtcattgcct
     9481 ggtcgcgcgt ttatcttggc gtgcactggc cgctggatat gctcggtgga ttgctggcag
     9541 gtatgattgg ctgccttagc gctcagatta tctggcaagc gatggggcat aaactctatc
     9601 aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa ggctgggtgc
     9661 gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa ctattcagag
     9721 ggattatgga aacacgtcgc gaagagcgta tcgggcagct gctgcaagaa ttaaaacgca
     9781 gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag atgacgattc
     9841 gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat attgttctgg
     9901 aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg gtggaagaaa
     9961 aacgccgggc ggcaaaaatg gctgcgacac tggtagaacc cgatcagact ctcttttttg
    10021 actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc ccttttaccg
    10081 ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat tgccgcgcat
    10141 ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaacccatc gattttcagc
    10201 aaacgttgaa taatttttgc ccggatatcg ctttttattc cgcggcgggc gtgcatgtca
    10261 gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg gccatgtcga
    10321 tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg cgtccggcgc
    10381 gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg gaagatgagt
    10441 atgtgaagta cgcgcagacg caccgtatta agttgatgta ttaatgacgt ataaccggat
    10501 gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga accactgatg
    10561 gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt ccacattttc
    10621 catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat caatggtccc
    10681 gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg tgtagctcgc
    10741 cttcaggttt ttcagctgcc cacgcggtat ggtcactgag cccgcttcac ctgccccaag
    10801 attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg catcaggttt
    10861 aattggcgtc acggtttcaa agaagcggaa accccaggtc aataatttct cagactcatt
    10921 aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca tatcgccctg
    10981 ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg gttcctgtct tcatgccatc
    11041 aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa ttttgttgaa
    11101 ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga tcaatgcttt
    11161 acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca gaccgtgcac
    11221 cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca tcaaaccaat
    11281 aaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt cattaccgga
    11341 ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa
    11401 cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt taccgaccgt
    11461 caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca catagctggt
    11521 catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt ctgccagcac
    11581 tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac
    11641 ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc
    11701 aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat ccattcaaaa
    11761 aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac acaaacttaa
    11821 agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc ttcgctgctt
    11881 ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac catcggagtc
    11941 ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg
    12001 aataattcaa ctaacgtaaa aaaagtattg ctgacgctcg aagaactgga actaccttat
    12061 gagcaaattc tcgcgggccg tgagtttggg ataaatcacg atgctgattt tctggcgatg
    12121 aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa
    12181 tcaaacgcca ttgtccgcta tctggcggca cagtacgggc aaaaacgcct gtggatcgac
    12241 tcaccggcac gtcgtgcgga agcggaaaaa tggatggact gggcaaacca gacactcagt
    12301 aatgctcatc gcgggatcct gatgggatta gtcagaacac cacccgaaga gcgcgatcag
    12361 gccgccattg atgccagttg caaagagtgc gacgccctgt ttgccctgct cgatgcggaa
    12421 ctggcaaaag taaaatggtt ctctggcgac gagtttggtg tgggcgatat cgctatcgca
    12481 ccgttcatct acaatttgtt taacgttggc ctgacctgga caccgcgtcc aaatctgcaa
    12541 cgctggtatc agcaactcac tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt
    12601 agctaattgc gtgggctaac tttaagtaat tccccactgg actcgtcggt gagaacgtat
    12661 aaataaccgt cgggtccaga gcgaacatca cgaattcgct gccctctgtc cgttaaaata
    12721 cggccatctt ctgtcacttt gtcgccgttg acgctcatca caatgacatc tttatctttc
    12781 agcgcgccta taaataattt ttgctgccac tgggggaatt tatcgctgtt atagaaggcc
    12841 atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt cccggcgacg
    12901 atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca ggttgccagc
    12961 ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc gcgcgggcca
    13021 tgttcattca gccacagtgc attactccac ggattcatcg ccattccttg cggattacga
    13081 atgccataag accagatctc ggcgcgcgca ccggattcct ttataaaagg attatcatcc
    13141 gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc cagatcctgc
    13201 gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt accgtcgaat
    13261 accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg gaagacggtg
    13321 cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac agcagttccg
    13381 gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa
    13441 tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac gtccggaact
    13501 ccggaaagcg gcgcagataa tccttttcct acttgccagt gacgcaactc gccgccgcgc
    13561 agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg atggtcgagt
    13621 ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc ggaagaaaga
    13681 caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctcagga ttgactctgg
    13741 cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact taaacatgaa
    13801 gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg tgcttctgca
    13861 ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg gcgttgacgg
    13921 taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct gtcgctaatg
    13981 cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga ttcaaccgca
    14041 gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga tgattgcttt
    14101 cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcggcg aggtcaacgc
    14161 gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac atacttcgat
    14221 cctcctcttt ccagccttgt tgcaattaaa tagcatggct attttttgag caaaatgctg
    14281 tccaggatca attcttcacg ccaaattaac aaaatttaca tttccttcgc ctccgccagc
    14341 gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc ctgacctttc
    14401 ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa aatcggcttt
    14461 gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac cgaactacgc
    14521 actgaaggtt atgacgtggt accgagctat gacgatgcgg acatggtgat cgtcaacacc
    14581 tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga agcgttgaat
    14641 gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca gatccgcgaa
    14701 gtccacccga aagtgctgga aatcaccggg cctcatagct atgagcaggt tctggagcac
    14761 gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt gccagaacaa
    14821 ggtgtgaaac tgacaccgcg tcattatgcc tatctgaaaa tttcagaagg ctgtaatcac
    14881 cgctgcacct tctgcattat tccgtctatg cgcggcgacc tggtgagccg tccgattggc
    14941 gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat tctggtgatc
    15001 tcgcaggata cttccgccta tggcgttgat gttaaacatc gtactggctt ccacaacggc
    15061 gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg
    15121 acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc actgatggca
    15181 gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc gcgcattctc
    15241 aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc
    15301 gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc tggcgagaca
    15361 gaagaagatt tccagatgct actcgacttc ctgaaagaag cgcgtctgga tcgcgttggc
    15421 tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga ccaggttccg
    15481 gaagaagtga aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc
    15541 gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga agtggacgaa
    15601 gaaggcgcaa ttggtcgcag catggcagat gcaccggaaa tcgacggcgc ggtttatctc
    15661 aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat
    15721 gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc
    15781 ggcctgcgaa taatgattac tgccagctaa tctgtgccct gccgtttttc ttactcttat
    15841 acagccgctc atccgccagc attttcaagc gttcgacggt aggacattca tttgaatgtg
    15901 ctacaccgat actggtactt aaattaatgc ttcccacctg gaaatgaacg atttctttta
    15961 gaatcgatag caactcttcc agcgttccac ccgtataaat caccgcaaat tcgtcaccgc
    16021 cgaagcggta gactttacct ttatctaccg ggatctgtga tttaaggatt ttcaccactt
    16081 tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat
    16141 cgacgtcaaa caggcacaaa taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa
    16201 aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta tacatactgt
    16261 agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat
    16321 ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg cgtaccagat
    16381 agaggcaact ggcacccgat atcactgcca ccaatgcggc aaagaaaata atcaatgtac
    16441 aaacccgcaa ataaacatct tttttatacg caaatacata caatccggtc ggcaaatagg
    16501 cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcatta tattcatcac
    16561 cggtcaccgg tttaccgttc ttaaacagag agaacatcag gttctcgctg tccatcgcca
    16621 gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt
    16681 gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat
    16741 ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact
    16801 cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg tacaagtaag
    16861 agggtgaaac cgagtcagcg taagctgtgc gtaagccaac caacagctgt tcctccgtgg
    16921 gatttagcgt attgataaga tcaattgcca cgctacgccg atattcagca atatttgcca
    16981 ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt
    17041 gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa
    17101 gtacgaactt attgattctg gacatacgta aactactctt ttactaattt tccactttta
    17161 tcccaggcgg agaatggcat gggtttctgg tacagatacc cttgggcctg aacacatcct
    17221 aatttgatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc
    17281 cggttatagc gcgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata
    17341 tcgccggtaa attgcgagtc cacttttatg ctatctggat taatttcata aaaacgtttc
    17401 aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt
    17461 tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca
    17521 acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga
    17581 aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc
    17641 agcgacgcac gctgactaaa atgtctaaat tcacccattg ccgttttaat aatctgataa
    17701 cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg
    17761 atcccccgct ccgcagacac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg
    17821 ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga
    17881 atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc atcacaaaaa
    17941 ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg
    18001 caactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga
    18061 ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc
    18121 tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag
    18181 cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt attgcgttca
    18241 tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc
    18301 agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc gctttcctga
    18361 tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc
    18421 gcgatcgaat ttttgatgct gataatctct ttcgcatcgc cttcgcgaat tttcagagcc
    18481 tcgatttgat ccgttgagtc gggcaaaaac gccagcgtat taaccagctc ggtcatttca
    18541 gaaaattgcc gacggaacac acgagtggtc atgaaatata ccaccagcaa cacgataata
    18601 atgaaagcaa acggtagata aaaaagttta tctgatgcgt ccaataaatt accgccagga
    18661 taataaaaat agatttcgaa caatgcatta tttggcactg ctgatgtctt tgcaatatac
    18721 tccgtatcga cagaatcaat aatattataa ttaccttcgc tattataaat aatcagtttt
    18781 tcacgagcga gttgattctt ggcaatgatc tcatgacctt gtttcaaaaa tagaacttca
    18841 tcattagcgc tgatttgatc aagatactcg ttcaga
//
LOCUS       DAHBHC010000094        18704 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHBHC010000094 DAHBHC010000000
VERSION     DAHBHC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.094"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(45..2720)
                     /gene="adhE"
                     /locus_tag="M2A35_004111"
                     /gene_synonym="adhC"
     CDS             complement(45..2720)
                     /gene="adhE"
                     /locus_tag="M2A35_004111"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCB7200337.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3196..3843
                     /gene="ychE"
                     /locus_tag="M2A35_004112"
     CDS             3196..3843
                     /gene="ychE"
                     /locus_tag="M2A35_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCB7200338.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(4001..4297)
                     /locus_tag="M2A35_004113"
     CDS             complement(4001..4297)
                     /locus_tag="M2A35_004113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200339.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            4581..6212
                     /gene="oppA"
                     /locus_tag="M2A35_004114"
     CDS             4581..6212
                     /gene="oppA"
                     /locus_tag="M2A35_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCB7200340.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEARRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6298..7218
                     /gene="oppB"
                     /locus_tag="M2A35_004115"
     CDS             6298..7218
                     /gene="oppB"
                     /locus_tag="M2A35_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCB7200341.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7233..8141
                     /gene="oppC"
                     /locus_tag="M2A35_004116"
     CDS             7233..8141
                     /gene="oppC"
                     /locus_tag="M2A35_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCB7200342.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQESLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8153..9166
                     /gene="oppD"
                     /locus_tag="M2A35_004117"
     CDS             8153..9166
                     /gene="oppD"
                     /locus_tag="M2A35_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCB7200343.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9163..10167
                     /gene="oppF"
                     /locus_tag="M2A35_004118"
     CDS             9163..10167
                     /gene="oppF"
                     /locus_tag="M2A35_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCB7200344.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10220..10549)
                     /locus_tag="M2A35_004119"
     CDS             complement(10220..10549)
                     /locus_tag="M2A35_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCB7200345.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10584..12044)
                     /gene="cls"
                     /locus_tag="M2A35_004120"
     CDS             complement(10584..12044)
                     /gene="cls"
                     /locus_tag="M2A35_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCB7200346.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12187..12360
                     /locus_tag="M2A35_004121"
     CDS             12187..12360
                     /locus_tag="M2A35_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCB7200347.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12415..13668)
                     /gene="kch"
                     /locus_tag="M2A35_004122"
     CDS             complement(12415..13668)
                     /gene="kch"
                     /locus_tag="M2A35_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCB7200348.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13968..14264)
                     /locus_tag="M2A35_004123"
     CDS             complement(13968..14264)
                     /locus_tag="M2A35_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCB7200349.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14488..15207
                     /gene="tonB"
                     /locus_tag="M2A35_004124"
     CDS             14488..15207
                     /gene="tonB"
                     /locus_tag="M2A35_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCB7200350.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(15247..15645)
                     /gene="yciA"
                     /locus_tag="M2A35_004125"
     CDS             complement(15247..15645)
                     /gene="yciA"
                     /locus_tag="M2A35_004125"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCB7200351.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15750..16289)
                     /locus_tag="M2A35_004126"
     CDS             complement(15750..16289)
                     /locus_tag="M2A35_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCB7200352.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16319..17062)
                     /locus_tag="M2A35_004127"
     CDS             complement(16319..17062)
                     /locus_tag="M2A35_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCB7200353.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(17090..17179)
                     /locus_tag="M2A35_004128"
                     /pseudo
     CDS             complement(17090..17179)
                     /locus_tag="M2A35_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17419..18057
                     /gene="ompW"
                     /locus_tag="M2A35_004129"
     CDS             17419..18057
                     /gene="ompW"
                     /locus_tag="M2A35_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCB7200354.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18117..18623)
                     /locus_tag="M2A35_004130"
     CDS             complement(18117..18623)
                     /locus_tag="M2A35_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCB7200355.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKIAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
BASE COUNT         4934 a         4360 c         4556 g         4854 t
ORIGIN      
        1 cggccccaga aggggccgtt tatattgcca gacagcgcta ctgattaagc ggattttttc
       61 gcttttttct cagctttagc cggagcggct tcttttttcg ctgcagtttc accttctaca
      121 taatcacgac cgtagtaggt atccagcagg atctgtttca gctcggagat cagcgggtaa
      181 cgcgggttag cgccggtgca ctggtcatcg aacgcatctt cagacagttt atccacgttc
      241 gccaggaagt ctgcttcctg aacgccagct tcacggatag atttcggaat acccagttca
      301 gctttcagcg tttccagcca tgccagcagt ttctcgatct tagcagcagt acggtcgccc
      361 ggtgcgctca gacccaagtg gtcggcaatt tcagcataac gacggcgagc ctgcggacgg
      421 tcatactggc tgaatgcagt ctgcttggtc gggttgtcgt tcgcattgta gcgaataacg
      481 ttacaaatca gcagggcgtt tgccagaccg tgcggaatat ggaactggga acccagtttg
      541 tgcgccattg agtgacatac acccaggaag gcgttcgcaa acgcgatacc cgcgatagtc
      601 gctgcactgt gaacacgttc acgcgctacc ggatttttag acccttcgtg gtaggacgct
      661 ggcagatatt ctttcagcag tttcagtgcc tgcagagcct gaccatcaga gaactcagat
      721 gccagtacag aaacataagc ttccatggcg tgagttactg cgtccagacc accgaaagca
      781 cacagggact tcggcatgtc cataaccagg ttggcgtcga caatcgccat atccggagtc
      841 agcgcatagt ctgccagcgg atatttctga ccagtagcgt cgtcagttac aaccgcaaac
      901 ggagtgactt cagaacctgt accagaagtg gtggtgacag cgatcatttt cgctttcacg
      961 cccattttcg ggaacttgta gatacgttta cggatatcca taaagcgcag cgccagctct
     1021 tcgaagtgag tttccggatg ttcgtacata acccacatga tcttcgcggc gtccatcggg
     1081 gaaccaccac ccagcgcgat aatcacgtct ggtttgaagg agtttgccag ttctgcacct
     1141 ttacgaacga tgctcagggt cgggtccgct tctacttcga agaagacttc agtttcaacg
     1201 cctgctgctt tcagtacgga agtgatctga tcagcataac cattgttgaa caggaagcgg
     1261 tcagtcacga tgagcgcacg tttgtggcca tcagtaatca cttcatccag cgcgattggc
     1321 agggagccac ggcggaagta gatagatttc ggaagtttgt gccacaacat gttttcagct
     1381 cgcttagcaa cggttttctt gttgatcagg tgtttcggac caacgttttc agagatggag
     1441 ttaccacccc aagaaccaca acccagagtc agggaaggtg cgagtttgaa gttatacagg
     1501 tcaccgatac caccctgaga cgctggggtg ttaatcagga tacgagccgt tttcattttt
     1561 tgaccgaagt aagaaacgcg agccggttgg ttatcctggt cagtgtacag gcaagaggta
     1621 tgaccgatac cgcccatagc aaccagtttc tctgcttttt ctaccgcgtc ttcgaaatct
     1681 ttagcgcggt acattgccag agtcggggac agtttttcat gtgcgaacgg ttcgctttca
     1741 tcaacaacgg tcacttcacc gatcagaatc ttggtgtttt ctggtacaga gaagcctgcc
     1801 agttcagcaa ttttataggc tggctgacca acgatagccg cgttcagcgc accgtttttc
     1861 aggataacat cctgaacagc tttcagctct ttaccctgca acagatagcc gccgtgggtt
     1921 gcaaaacgtt cacgtacagc gtcataaaca gagtcaacaa caacaacaga ctgttcagaa
     1981 gcacagatta cgccgttgtc gaaggttttg gacatcagta cagatgcaac tgcacgtttg
     2041 atatcagcag tttcatcgat aacaactgga gtgttgcccg cgcctacacc gatagctggt
     2101 ttaccggagc tgtatgcggc tttaaccatg cccggaccac cagtcgcgag gatcaggttg
     2161 atgtctgggt ggtgcatcag tgcgttagac agttcaacag aaggttgatc gatccagccg
     2221 atcagatctt tcggagcacc ggcagcgata gcagcctgca gaacgatatc agccgctttg
     2281 ttggtggcat cttttgcacg cgggtgcggg gagaagataa tggcgttacg ggtcttcaga
     2341 ctgatcagcg atttgaagat agcagttgaa gtcgggttag tggtcggaac gataccgcaa
     2401 ataataccga ttggttcagc gatagtgatg gtaccaaaag tgtcgtcttc agacagaaca
     2461 ccacaggttt tttcatcttt ataggcgttg tagatatatt cagaagcaaa gtggtttttg
     2521 atcactttat cttcgacgat acccatgccg gattcggcaa cggccatttt cgcgagtggg
     2581 attcgagcat ctgcagcagc cagagcggcg gcgcggaaga ttttgtctac ttgctcttga
     2641 gtgaaactgg catattcacg ctgggctttt tttacacgct ctacgagtgc gttaagttca
     2701 gcgacattag taacagccat aatgctctcc tgataatgtt aaactttttt agtaaatcat
     2761 ctgctcgaat acgagagtat agtcagtgcg gtgatgattt gcttaaccta tgaaaatcaa
     2821 aagcttactc gcgctcacac tcactgtgat ttactaaaag agtttaaaca ttagagttat
     2881 tatctctaat gcgtcacttc caggtggcta agcaagatta ctcacttctg ggtactgatt
     2941 acgtgatcca aatcaaattt ttgcaaagct gacacctttc agcatcgctt ttcgccatta
     3001 tagctaacag ttaataaatt gtagtatgat ttggtggcta cattagcatg ttttgcacaa
     3061 ctagataaca ataacgaatg atagcaattt taagtagtta ggaggtgaaa aatgctgtca
     3121 aaaggcgtat tgtcagcgcg tcttttcaac cttatttatg gctaacatta tccggctttt
     3181 gcttcggagc taaccgtgat tcagaccttt tttgattttc ccgtttactt caaatttttc
     3241 atcgggttat ttgcgctggt caacccggta gggattattc ccgtctttat cagcatgacc
     3301 agttatcaga cagcggcagc gcgaaacaaa actaacctta cagccaacct gtctgtggcc
     3361 attatcttgt ggatctcgct ttttctcggc gacacgattc tgcaactttt tggtatatca
     3421 attgattcgt tccgtatcgc cgggggcatc ctggtggtga cgatagcgat gtcgatgatc
     3481 agcggcaagc ttggcgagga taaacagaac aagcaagaaa aatcagaaac cgcggtacgt
     3541 gaaagcattg gtgtggtgcc attggcgttg ccgttgatgg cggggccagg ggcgatcagt
     3601 tctaccatcg tctggggtac gcgttatcac agcattagct atctgtttgg tttctttgtg
     3661 gctattgcat tgttcgcttt atgttgctgg ggattgttcc gcatggcacc gtggctggta
     3721 cgggttttac gccagaccgg catcaacgtg attacgcgta ttatggggct attgctgatg
     3781 gcattgggga ttgaatttat cgttactggt attaagggga ttttccccgg cctgcttaat
     3841 taattccttt caaatgaaac ggagctgcca tgctccgttt acttcgtcat tatttttact
     3901 ttgttcccgc gcagttatca aaagcaaaag gaataggtaa aaatattctt ctcaaattac
     3961 agttagttat aaggatttcc ttaactgctt ctcctcacca tcatgttatt ttcgccacat
     4021 cataatcctg ggcttgctga agaataattg aaatgatatt agtaattcca ctgcctttgg
     4081 tagaggaaag tgctaaataa taatcaattg ttaaattatt gtgcatttca ctactggaac
     4141 tgtaatcaga aaagatagac atgcttagcc aatctctatt tgattgaatt gaaagatgtt
     4201 tgttaaggca tggatgcaag ctatagattc tgatacggtc aataaaagag aattgcttaa
     4261 caattttgca aaatgtattg gcgagtaaga accgcatttg gtactttccg ggcaaccgcc
     4321 agacgattct ttattggtaa tgagaataat taacaattaa agagcgtcgc gaaagaataa
     4381 tgtgtctcga caggggagac acagtacgaa tcgacataag gtgatcgtct gaatcaccag
     4441 aataaataaa gtcggtgata gtaatacgta acgataaagt aacctgacag cagaaagtct
     4501 ccgagcctgt gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata
     4561 atgagggagt ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg
     4621 ttctggctgc gctaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca
     4681 cactggcgga aaaacaaaca ctggtacgta acaatggttc agaagttcag tcattagatc
     4741 cgcacaaaat tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac
     4801 tggtcagcga tcttgacggt catccagcac ctggcgtcgc tgaatcctgg gataataaag
     4861 acgcgaaagt ctggaccttc catttgcgta aagatgcgaa atggtctgat ggcacgccag
     4921 tcacagcaca agactttgtg tatagctggc aacgttctgt tgatccgaac actgcttctc
     4981 cgtatgccag ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga
     5041 aaaaaccgat taccgatctc ggcgtgaaag ctattgatga tcacacatta gaagtcacct
     5101 taagtgaacc cgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc
     5161 caaaagccgc tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcgtcacca
     5221 acggtgccta taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc
     5281 cgacctactg gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt
     5341 ctgaagttac cgatgtcaac cgctaccgta gtggtgaaat cgacatgact tataacaaca
     5401 tgccgatcga attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc
     5461 catacctgtg cacttactat tacgaaatta acaaccagaa accgccattc aacgatgtgc
     5521 gtgtgcgtac cgcactgaaa ctaggtatgg accgcgatat cattgttaat aaagtgaaag
     5581 cgcagggcga catgcccgcc tatggttaca ctccaccgta tactgatggc gcaaaattga
     5641 ctcagccgga atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc
     5701 tggctgaagc gggttatacc gcagacaaac cgttgaccat caacctgttg tataacacct
     5761 ccgatctgca taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg
     5821 taaacgtcaa actggttaac caggagtgga aaacgttcct cgacacccgt caccagggta
     5881 cttttgatgt ggcccgtgca ggctggtgtg ctgactacaa cgaaccaact tccttcctga
     5941 acaccatgct ttcgaacagc tcgatgaata ccgcgcatta taagagcccg gcctttgaca
     6001 gcattatggc ggaaacgctg aaagtgactg acgaggcgcg gcgcacagct ctgtacacta
     6061 aagcagaaca acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg
     6121 cgcgtctggt gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct
     6181 atacccggaa tatgtacatt gtgaagcact aatggcaata cgtggggcag gagtgtcctg
     6241 ctccacggtg tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg
     6301 ttaaaattta ttctacgtcg ctgtctggaa gcgattccga cgctatttat tcttataact
     6361 atttcgttct ttatgatgcg cctcgcgccg ggaagccctt ttaccggcga acgtacttta
     6421 ccgccagaag tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca
     6481 cagtatttca gctacctgaa acaactggcg cacggtgatt tcggtccatc gtttaaatat
     6541 aaagattatt cggtcaatga cttggttgca tccagttttc ccgtttctgc caaactggga
     6601 gccgcagcat ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgccgcatta
     6661 aaacaaaaca ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc
     6721 cccagttttg tggttgcacc attattagtc atgatatttg cgatcatttt gcattggctg
     6781 ccgggcggtg gctggaatgg tggggcgctt aaattcatga tattgccgat ggtggcgttg
     6841 tcactcgctt atatcgccag tattgcgcgt attacccgtg gctctatgat tgaagtatta
     6901 cactccaatt ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta
     6961 cgtcacgcat taaaacctgc tctgttaccc gtgctctctt atatgggccc cgcatttgtc
     7021 ggcattatta ccggttctat ggttatcgaa accatttatg gtttgccggg gattggacaa
     7081 ctgttcgtta atggcgcatt gaaccgtgac tattccttag tgttaagcct gaccatcctg
     7141 gttggtgctt taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac
     7201 ccgaaaattc gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga
     7261 cgctggaaaa tttcagtgaa aagctggagg tcgaagggcg cagcttgtgg caggacgcac
     7321 gtcgacgttt tatgcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctgatcg
     7381 cgttatttgt aatcctggca ccgatgcttt cccagtttgc ctatgacgat actgactggg
     7441 cgatgatgtc cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg
     7501 gtcgcgacct gcttgtgcgc gttgcgattg gcgggcgtat ctcactcatg gtcggtgttg
     7561 ctgcggcact ggtggctgtg gtcgtcggga cactttatgg ttcgctttcc ggttatctgg
     7621 gcggtaaagt ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca
     7681 tgttcttcgt cattttgctt gtgacctttt tcggtcaaaa catcctgctg attttcgtgg
     7741 cgattggcat ggtttcctgg ctggatatgg ctcgtattgt gcgtgggcaa accctgagtc
     7801 tgaagcgcaa agagtttatt gaggcggcac aagttggcgg tgtatcgaca tcgggcattg
     7861 ttattcgcca cattgtgccg aatgtactcg gtgtggtggt ggtctacgca tcacttctgg
     7921 tgcccagcat gatcctcttt gaatctttcc ttagcttcct ggggttgggt acgcaagagt
     7981 cgttaagcag ctggggtgca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat
     8041 ggttattgtt gttcccagcg ggattcctcg tggtgacgct gttttgtttc aactttatcg
     8101 gcgatggctt gcgtgatgcc ctcgacccga aagatcgtta aggagtgcag ccatgagcgt
     8161 aattgaaact gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa
     8221 agatttgcgt gtcaccttta gtaccccgga cggcgacgtc acggcggtca atgatttgaa
     8281 tttttcccta cgtgccggag aaacgctggg tattgtaggt gagtctggtt cgggtaaatc
     8341 gcaaactgca tttgcgttga tgggcctgtt ggctgccaac ggacgtattg gcggatcggc
     8401 aaccttcaat gggcgtgaaa tcctcaattt gccagagcat gaactcaata aactgcgcgc
     8461 tgaacaaatc tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt
     8521 cggtgagcag ttaatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt
     8581 tgaagagtcg gtgcggatgc tcgatgcggt aaaaatgccg gaagcgcgta aacgcatgaa
     8641 aatgtacccg cacgaatttt ctggcggcat gcgtcagcga gtcatgattg cgatggcatt
     8701 gctatgtcga cctaagctac tgattgcgga tgaacccact actgcgctgg acgtcaccgt
     8761 acaggcgcag atcatgacgc tattaaatga actgaagcgg gaatttaata ccgccatcat
     8821 tatgatcacc cacgatcttg gcgtggtggc ggggatctgc gacaaagtgc tggtaatgta
     8881 cgccgggcgc acgatggaat atggcaatgc gcgcgatgtc ttttatcaac ccgttcatcc
     8941 ttattctatc ggtttgctca acgcggtgcc gcgtcttgat gcggaaggtg aaacaatgtt
     9001 gaccatcccg ggtaatccgc caaacttgct gcgattaccg aaaggttgcc cgttccagcc
     9061 acgttgtcca catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc
     9121 tggccgtctg cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaactgaa
     9181 ggaagaaaag tcctccttga aatcgccgat cttaaagtgc actttgaaat caaagatggc
     9241 aaacagtggt tctggcaacc gccgaaaacg ctcaaagccg tcgatggtgt cactcttcgc
     9301 ctgtatgaag gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt
     9361 gctcgcgcca tcattggctt ggttaaagcg accgacggtc atgttgcctg gttaggtaaa
     9421 gagttgctgg gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt
     9481 ttccaggatc cgttggcatc gctaaacccg cgtatgacca tcggcgagat catcgctgaa
     9541 ccactgcgta cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg
     9601 atgatgctga aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctcc
     9661 ggtgggcagt gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt
     9721 atctgcgatg agccggtttc ggcgctggac gtgtcaattc aggcgcaggt ggtcaacctg
     9781 ctccagcagc tgcaacgtga gatgggattg tcattaattt ttatcgctca tgacctggcc
     9841 gtggtaaaac acatttccga tcgtgtgttg gtgatgtatc tcggccatgc ggtagaactg
     9901 gggacctatg atgaggtcta ccacaatcca ctacatcctt acaccagggc attgatgtcg
     9961 gcagtcccca tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg
    10021 gaattaccgt cgccgatcaa cccgccttcc ggttgtgttt tccgtacccg ttgcccgatt
    10081 gccggtccgg agtgcgccaa aacacgtcct gttctggagg ggagtttcag acacgccgtt
    10141 tcttgcctga aagtcgatcc gctttaaaca caataagggc tgacagttgt cagccctttt
    10201 tcacgctaaa agcgattatt tattcccgcc agatgatatg gcaaagtttg tgatcttttt
    10261 cgcggcataa cagaatgcgg gcgaaaacat cgttgatttc accatcttca ctgtccgcca
    10321 gaccaatcac cacttcggca aaaaagtctg ggttcaaatc gaaatcaaca tgttcctgcc
    10381 agtcttccgc cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca
    10441 gcagaacatc agctggatcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct
    10501 gctcaagcgt ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct
    10561 gtttaacctc tgttggcgac gttttacagc aacggactga agaagtaaaa cagtcgctcg
    10621 gcgacacgtt gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc
    10681 gaaatataat cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc
    10741 agggtaatct cgaaattcag ccacagacta cgcatatcaa ggttaacggt gccaaccaga
    10801 cttagttcgc catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa
    10861 attttaaccc cagcagcaag aagttccgta aagaatgcgc gactggccca gccgaccagc
    10921 atcgagtcat tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg
    10981 caaatcgcat gaagtaaatc atcgcttggc acaaagtagg gcgtggtcat gatcaaatat
    11041 tcacgcgccg aataagccgc agtcaataat gcctggtgaa tgagatcttc cggaaagccg
    11101 gggccagaag caattgtgtg aatggtgtga ccgctggcct gttcaaacgg cataatattg
    11161 acatctggtg gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa
    11221 ataatcccca tcgcggtggc gatggggcct tccatacgcg ccatcagatc aatccattgc
    11281 cctacgcccg catcttgttt gaagtagcga ggatcgacca tattcatgct gccggtgtac
    11341 gcgatgtaat tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa
    11401 aacacacgca tcagattgac ctttaaggct tcgaccactt caataccggc attacgcatt
    11461 agctcgggcc acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg
    11521 caatgaatac cacgccgtgc agccgccatt aatgattcag ccacctggtc cgccatgccg
    11581 ccgggctgcc agatataaaa caccatctca atattatggc gcgcgagctg gatgtcgcgg
    11641 attaacgcct gcatcacatc atctgactcg gtcatcagtt gtagctgatt ccctttgacc
    11701 ccagcgatcc cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt
    11761 tcttcggcga agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc
    11821 cacatcgctc tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga
    11881 taggcaataa ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag
    11941 ggaactgcgc gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat
    12001 cccagaatgg ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt
    12061 tgaaagcgca ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga
    12121 aaagcactgg tcagcgtcgg gggtgggggt ataatgacca ttctgttatt gcatagagta
    12181 gttaacatga agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc
    12241 cgacgtaaat cgcgttggct tgaggggcaa tcgcgccgaa atatgcgtat ccacagcatc
    12301 aggaagtgca ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa
    12361 atgtactggg caccattgcg gtgcccaata agtttactct tccgtcttta aactctattt
    12421 ttgcgccgat tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatacc
    12481 atgacctgag ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa
    12541 aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac
    12601 catcttaatc ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact
    12661 gctcatatct tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc
    12721 cagaatggcc cggcaacgat cgattcccgc tttctttaat actgaactgt cattactgtc
    12781 accggggata acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc
    12841 aggcaagttg ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt
    12901 gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt
    12961 gtttcctttt acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat
    13021 agatgtggca aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga
    13081 ttcagaaaca gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata
    13141 gaacgcggtc atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc
    13201 gtaggttgag taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc
    13261 ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca
    13321 aaatccaata ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa
    13381 caacaaaatg atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt
    13441 caggacaaga aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa
    13501 ctctgaaaat gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt
    13561 tttaaaaata agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgaagggt
    13621 cacccataaa tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt
    13681 aaccacaatt ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt
    13741 gaatgtgtta aacaatagta gatgacttta tccgcagtaa tcaaaggatt tgagcgagca
    13801 actgtacctc aggaaaggtc atttttcctg aatatgctca catcatataa agaaatacag
    13861 ataaagttat tatctgcttg tggtagtgaa tgcactgacc ggctataagg aaaggccaaa
    13921 caagaacacg gttgcaaaaa ccgtgccctt aaatattgaa tctctattca gaacactttc
    13981 ttaaatggtt tcactgaaac gtgttcatag actcctgccg ctacgtacgg gtcagcatcg
    14041 gcccaggcct gagctgcttc cagcgattca aattcagcaa taacggttga gccagtaaat
    14101 cccgcagccc ctggatcgtt actgtctacc gctggcattg gaccagctgt caacaaacga
    14161 ccttcatcat gcagtaactg taaacgtgct aaatgtgccg gacgaacgga aaggcgtttt
    14221 tcgaggctat cagctttgtc ttgagcgtaa ataacataca acacgggcac aactccttgt
    14281 tcgggaaagt tgtaaagtac gttatttgaa agggcgaaga tctgcaacgg aaagatgatg
    14341 tctttgttaa gaccatgcat aaattaaggt caattacgcc aaaaatgaca ttttcactga
    14401 tcctgatcgt cttgccttat tgaatatgat tgctatttgc atttaaaatc gagacctggt
    14461 ttttctactg aaatgattat gacttcaatg acccttgatt tacctcgccg cttcccctgg
    14521 ccgacgttac tttcggtctg cattcatggt gctgttgtgg cgggtctgct ctatacctcg
    14581 gtacatcagg ttattgaact acctgcgcct gcgcagccaa tttctgtcac gatggttgcg
    14641 cctgctgatc tcgaaccgcc acaagccgtt cagccgccac cggagccggt ggtagagcca
    14701 gaaccggaac ctgagccgat ccccgaacca ccaaaagaag caccggcggt cattgaaaag
    14761 ccgaagccga aacctaagcc aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa
    14821 cgcgatgtca aacccgtaga gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgca
    14881 cgcctgacat caagtacggc aacggctgca accagcaagc cggttaccag tgtggcttca
    14941 ggaccacgcg cattaagccg taatcagccg cagtatccgg cacgagcaca ggcattgcgc
    15001 attgaagggc aggttaaagt taaatttgac gtcacgccgg atggtcgcgt ggataacgta
    15061 caaatcctct cagccaagcc tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc
    15121 agatggcgtt atgagccggg taagccaggc agtgggattg tggtgaatat cctgtttaaa
    15181 attaacggca ccaccgaaat tcagtaagca gaaagtcaaa agcctccgac cggaggcttt
    15241 tgactattac tcaacaggta aggcgcgagg ttttccttca ggatcaaccg cgacatactt
    15301 aaataatgct tctgtcgctt tatagcgttg cccgattggt tcagacgcta cttttttcac
    15361 ccacacttca atattaatgc tgaccgatgt cgtccctttc tggacacagc gtgcatagca
    15421 gcacaccaca tcgccgaccg caaccggccg taagaaagtc attccttcaa cccgcacagt
    15481 cactacgcga ccgtgggcaa tttctttcgc cagaatagcg ccgccaatat ccatttgtga
    15541 cattaaccaa ccaccaaaga tgtcaccatt ggcattggta tcggcgggca tggctaaagt
    15601 acgtaaaaca agatcgccct gagggacgtt atgtgttgta gacatggtaa aaccgactta
    15661 aaaggaaaat cacaggcgcg atgctactat gattttcctg aggagaacag aggagataca
    15721 gcgccagccc cgaaggactg gcagtctggt taggatttat cttcctgcgg catgtggcgg
    15781 tagatataga taccgcttaa caatgtgaag attaaggtaa gggcggtcag gccaaagact
    15841 ttaaagttga cccaaatatt ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg
    15901 caaaggataa agaaaacagc ccaagccaga tttagcttcg accataccgg ttgcggcagc
    15961 gtgagttctt tacccagcat ccgctgaatt agcggctttt tcatcaccca ttggctgact
    16021 aacagggctc ccgcaaacag agcataaatg actgtaacct tccatttaat aaactcatca
    16081 ttgtggaaga acagcgtcaa gccaccgaag acgaccacca gaacaaaagt gatcagggcc
    16141 atcttctcaa ccttacgaaa gcgaacccag ctatagataa gcacaatcgc cgtggcgacg
    16201 atcagcgccg cagtagccgc atagatgtca taaatcttgt aaaacgcgaa aaagacaacc
    16261 agcggtaaaa aatcaagaaa ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt
    16321 attggcgaat caacatatac aggcggaaca gatagatgag caatatggca gaaatcaggt
    16381 tgctcaaggt gttcgccagt acggcaccaa tttccggggt taatgcggca aaagaagagg
    16441 caaaaagcag caacagtgtt tttgccagca accagctcag tactgcgggt gccaccagac
    16501 gcatattcgc ccaagtcagc cgcatactgc tacgcatcga ggcaaaaatg cccattttgt
    16561 cctgtaccag catcaccggt gccagggcca gtaaaatggc catgataatt cccggaacga
    16621 ccaccagcat gatgccaatc tgtactaaaa gggtagtcag aaaaatcaga ataaataact
    16681 tgggcaatat cggcgcactg gcaccaatag ctcgcagcgc actgactctc tgacccgcag
    16741 acaccaactg gataattaat attacgcctc cggcgagaat ggcgttaccg attaatcctg
    16801 aaaaagtgga cgccgctgaa gcctgcagca aaatttgttg ctgttccggt gacatattct
    16861 gaaccaggtc gaacaaccca ctactgccgc taacgggcac gccgtcattg agctgcgcaa
    16921 gctgtgcatc actgggtgag aaaacatgcc ctaacaccac tgtgataaac gcacatagca
    16981 acgataccaa cagaatggtc ataaattgat tacggaagaa atttccggtg tcacggtata
    17041 cggactgcgc cgtgatagac atgcactctc cttgagtttt atgcaggtgt taattagcgg
    17101 gcaattgtac cctggttaag cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat
    17161 ctttgtaaag cggaggtaat tgctggcctt caggcgtggg caaaccgcgg caagtggcga
    17221 tcgagttaat atttgcgtag agaaaatatt taaaaattga tttaaatcac attaaccagg
    17281 attctcaatg caacttctaa attaatccag atcaataaat ggtgaattat catatgtaat
    17341 gtgatctatg taggatcatt tgttactcca atgtaggtat attcgtcacg tttttataac
    17401 cataacgacg gagcggatat gaaaaagtta acagtggcgg ctttggcagt aacaactctt
    17461 ctttctggca gtgcctttgc gcatgaagca ggcgaatttt ttatgcgtgc aggttctgca
    17521 accgtacgtc caacagaagg tgctggtggt acgttaggaa gtctgggtgg attcagcgtg
    17581 accaataaca ctcaactggg cttgacgttt acttatatgg cgaccgacaa cattggtgtg
    17641 gaattactgg cagcgacgcc gttccgccat aaaatcggca cccgggcgac cggcgatatt
    17701 gcaaccgttc accatctgcc gccaacactg atggcgcagt ggtattttgg tgatgccagc
    17761 agcaaattcc gtccttacgt tggggccggt attaactaca ccaccttctt tgataatgga
    17821 tttaacgatc atggcaaaga ggcggggctt tccgatctca gtctgaaaga ttcctgggga
    17881 gctgccgggc aggtgggggt tgattatctg attaaccgtg actggttggt taacatgtca
    17941 gtgtggtaca tggatatcga taccaccgcc aattataagc taggcggtgc acagcaacac
    18001 gatagcgtac gcctcgatcc gtgggtgttt atgttctcag caggatatcg tttttaattc
    18061 cacaaaaaaa cgaccccgta atatacgggg tcaataagga catggcataa agtgtattat
    18121 ttcttcgctt ctacgccgtc agtttcagag cgaattaaaa atttctcagt tgtttgcgga
    18181 atatgctgaa tcagccagtc ggccatttgc ttttcctcat ttaaaatcgc ttcgatggtt
    18241 ggaattgaag ctgtatcacc ggcatttttt gctgctgcta atagtgaggt gtaacaggcg
    18301 atttcaaatt gctcgaagac atatccgcta atagagcctt tgactatttc atcagaaggg
    18361 aatataccac cgattgactg cccaagcgca gctattttac tcatggaatc tttaatgact
    18421 gaacgtgaaa tgtcattacg atcaagaata gtttccagtt gaacaatctg gtttttggtt
    18481 tcactaagat gttgttcaat acgagcgcgt agttcaggat aattatctat acggctggcc
    18541 atggattcaa gcatagattc ggcttgcttt tccattgcgt gggcgtcacg taaccagtca
    18601 tgataatgtt caatacgatt catgaaaatg tcctctttat aaaatgacta aaagttaaag
    18661 ttcatatttc aggctttatt ttcggctttc ttatttacgt tatt
//
LOCUS       DAHBHC010000095        18685 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHBHC010000095 DAHBHC010000000
VERSION     DAHBHC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.095"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..639
                     /locus_tag="M2A35_004131"
     CDS             <1..639
                     /locus_tag="M2A35_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HCB7200356.1"
                     /translation="RESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLV
                     EGETETWVINELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEA
                     GKKYAATVRSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNL
                     RKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(636..1592)
                     /locus_tag="M2A35_004132"
     CDS             complement(636..1592)
                     /locus_tag="M2A35_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCB7200357.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            1743..2858
                     /gene="macA"
                     /locus_tag="M2A35_004133"
     CDS             1743..2858
                     /gene="macA"
                     /locus_tag="M2A35_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCB7200358.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            2855..4801
                     /gene="macB"
                     /locus_tag="M2A35_004134"
     CDS             2855..4801
                     /gene="macB"
                     /locus_tag="M2A35_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCB7200359.1"
                     /translation="MTPLLELKDIRRSYPASDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVIERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(4874..5098)
                     /gene="cspD"
                     /locus_tag="M2A35_004135"
     CDS             complement(4874..5098)
                     /gene="cspD"
                     /locus_tag="M2A35_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCB7200360.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            5421..5741
                     /gene="clpS"
                     /locus_tag="M2A35_004136"
     CDS             5421..5741
                     /gene="clpS"
                     /locus_tag="M2A35_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCB7200361.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5772..8048
                     /gene="clpA"
                     /locus_tag="M2A35_004137"
     CDS             5772..8048
                     /gene="clpA"
                     /locus_tag="M2A35_004137"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCB7200362.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(8392..8479)
                     /locus_tag="M2A35_004138"
     tRNA            complement(8392..8479)
                     /locus_tag="M2A35_004138"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8443..8445),aa:Ser,seq:gga)
     gene            complement(8733..8951)
                     /gene="infA"
                     /locus_tag="M2A35_004139"
     CDS             complement(8733..8951)
                     /gene="infA"
                     /locus_tag="M2A35_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCB7200363.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(9236..9940)
                     /gene="aat"
                     /locus_tag="M2A35_004140"
     CDS             complement(9236..9940)
                     /gene="aat"
                     /locus_tag="M2A35_004140"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCB7200364.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(9982..11703)
                     /gene="cydC"
                     /locus_tag="M2A35_004141"
     CDS             complement(9982..11703)
                     /gene="cydC"
                     /locus_tag="M2A35_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCB7200365.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11704..13470)
                     /gene="cydD"
                     /locus_tag="M2A35_004142"
     CDS             complement(11704..13470)
                     /gene="cydD"
                     /locus_tag="M2A35_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCB7200366.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13593..14558)
                     /gene="trxB"
                     /locus_tag="M2A35_004143"
     CDS             complement(13593..14558)
                     /gene="trxB"
                     /locus_tag="M2A35_004143"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCB7200367.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            15103..15597
                     /gene="lrp"
                     /locus_tag="M2A35_004144"
     CDS             15103..15597
                     /gene="lrp"
                     /locus_tag="M2A35_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCB7200368.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15732..>18685
                     /gene="ftsK"
                     /locus_tag="M2A35_004145"
     CDS             15732..>18685
                     /gene="ftsK"
                     /locus_tag="M2A35_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCB7200369.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAVAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFR"
BASE COUNT         4687 a         4675 c         5012 g         4311 t
ORIGIN      
        1 cgtgaatctt ctcgtgttgc cgcctggcgt ctggggccaa gtggcttgag cactgaagac
       61 agccgccgca tctcttttca cattcgtttt aatcgtccgt catcgctgtt tgctcgctgc
      121 tggttgctgg tggaagggga aacagaaacc tgggttatca acgaactggc gcgccagtgt
      181 ggacaccatt tcgatgccga agggatcaag gtcattgagt ttgcccagtc cgggctaaag
      241 ccactggtta aatttgcccg ccgaatgggg attgaatggc atgtactggt cgatggcgat
      301 gaagcaggga agaaatatgc cgctacggta cgcagcctgt tgaataacga tcgggaagcc
      361 gaacgagaac atttaacggc gttaccggcg ctggatatgg aacattttat gtatcgccag
      421 ggattttccg atgtgttcca ccgcgtggcg caaatcccgg aaaatgtacc gatgaatctg
      481 cgcaaaatta tctcgaaagc gatccatcgc tcttccaaac ccgatcttgc cattgaagtg
      541 gcaatggagg ccggacgtcg tggtgtggac tccgtaccga cgctgctgaa aaaaatgttc
      601 tcacgcgtgc tgtggctggc gcgcggtcgc gcggattaac cgcgaaacat cgtggccatt
      661 tgtggctgaa tagcgtcgag catctcatag cgccgacggt attcagcccg ttttttactg
      721 gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca
      781 tcacatacgc cgccaaccga ttcccagaaa gcgttgtaat cggcatggat cttgccttct
      841 ttatcacgat aacgcaggct gcggtaaata tgcgtttcat tgctgacggc aataatctgc
      901 tctacctgca aacgttgggc aaacagacag gccgcttcca tcacgaggcg tttgggaaat
      961 agcccgtggc aggctttcgt cgcattctgg atttcctgat gtggaatttc ccattttgcg
     1021 ccttgcagtc cgccaataaa catcgttctt ttcccctgat attcacacag ggtaaacgtg
     1081 atctctgcca gaggaatacc ttcgctgttg cgaaacagga ttgtgctgtc accttcttta
     1141 tccattgaga tcatcatggt cagctcaagc gtgaactgct cgccgttttt gccttccagc
     1201 ttcgccagtt gcagcccggg ggtattcaaa tataagctga attcttccgc cgacatacat
     1261 ccgcggagta acgcataatg gtaacgtaac gcctccagca attgcttacg gctaagattc
     1321 gccgcaaggt aagggcgatg cagacgcaca ggcagtcgcg gttggcgcgt taacaacaca
     1381 ttgagattag gccagtggga aagttcgttc atccactcaa cgcttaaacg cggcataatc
     1441 aacgagcgca gcaaaaattt ctggcgaaaa ctacggcgat gccagaattt acccggccga
     1501 cactgtccac gtgccagact aagaaaaagt gacaggctgc tgagagattc agatggcgta
     1561 aaggtccgtt cagttagctg cgacatattc atgaaatcaa tggttataca tgacgtcgat
     1621 ttcaccattg cgtatcttaa ccaaacatca atagtgtgat tactaacgta aattttaggg
     1681 ttttgttgat atttcgttga agttaatgac ccggattggc atatggagta ttcagaatat
     1741 ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat tgcgctggtg atagtcatcg
     1801 ccggactgat tacgttatgg agaattctta acgcacccgt gccgacttat cagacactga
     1861 ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc
     1921 gtaaggttga cgtgggcgcg caggtcagcg gtcagttgaa aactctgtcg gtggcgattg
     1981 gcgataaagt aaaaaaagac cagcttttag gggttattga tcctgaacag gctgaaaacc
     2041 agattaagga ggtcgaagca acgctgatgg agctacgtgc gcagcggcag caggcggaag
     2101 cggagctgaa actggcgcgg gtgacgtatt cccgtcagca acgtctggca caaacgcagg
     2161 cggtttcaca gcaggatctc gacaccgccg cgacggagat ggctgtgaaa caggcgcaaa
     2221 ttggcaccat tgacgcgcaa atcaagcgca atcaggcttc tctcgatacg gctaaaacca
     2281 atctcgatta cactcgcatc gttgccccga tggccgggga agtcacgcaa atcaccactc
     2341 tgcaaggcca gacggtgatt gccgcacaac aagcaccgaa cattctgacg ctggcagata
     2401 tgagcaccat gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg
     2461 ggcaaaaagc ctggtttacg gtgcttggcg atccactgac gcgctacgag gggcaaatca
     2521 aggatgtact accgacgccg gaaaaggtta acgacgctat tttctattac gcccgttttg
     2581 aagtccccaa ccccaatggt ttgctgcggc tggatatgac tgcgcaagtg catattcagc
     2641 tcaccgatgt gaaaaatgtg ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg
     2701 ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga
     2761 ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg gcttgaagcg ggcgatgaag
     2821 tggtgattgg tgaggccaaa ccaggagctg cacaatgacg cctttgctcg aattaaagga
     2881 tattcgtcgc agctatcctg ccagtgatga gcaggttgag gtgctgaagg gcatcaccct
     2941 cgatatttat gccggtgaga tggtagcgat tgttggcgct tcaggttccg gtaaatcgac
     3001 cctgatgaat attctcggct gtctggataa ggccaccagc ggcacctatc gcgtcgccgg
     3061 tcaggatgtt gccacactgg acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg
     3121 ctttattttc cagcgttacc atttactttc gcatttaacc gccgagcaga acgttgaagt
     3181 acccgccgtc tatgccggtc ttgagcggaa acagcggctg cttcgcgccc aggggttact
     3241 gcaacgactg gggctggaag accgtacaga gtattatccg gcacagcttt cgggtggtca
     3301 gcaacagcgc gtcagcatcg cgcgggcatt gatgaacggc ggtcaggtaa ttcttgccga
     3361 tgaaccaacc ggcgcactgg acagccattc tggcgaagag gtgatggcga tcctgcatca
     3421 gctgcgcgat cgcgggcata cggtgattat cgtcacccac gatccgcagg tcgctgctca
     3481 ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg cgcaatcctc ccgccattga
     3541 aaaagtgaat gttgctggcg ggacggagcc tgttgtcaac acggtgtctg gctggcggca
     3601 gtttgtcagc ggttttaacg aggcgctgac gatggcatgg cgggcgctgg cagcgaataa
     3661 aatgcgtact ttactgacca tgctggggat tattatcggt attgcgtcgg tggtttccat
     3721 tgtcgtggtg ggtgacgccg ccaaacaaat ggtgctggcg gatattcgtt ctattggtac
     3781 gaatactatt gatgtctatc ccgggaaaga ttttggcgat gacgatccgc aatatcagca
     3841 ggcgctgaag tacgacgact taatcgccat ccaaaaacaa ccgtgggtcg cctcagccac
     3901 acctgccgtc tcgcaaaacc tgcgcctgcg ttataacaat gttgatgttg ctgccagtgc
     3961 caatggcgtg agcggcgatt attttaatgt ctatggcatg accttcagtg aaggaaacac
     4021 ctttaatcag gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg
     4081 ccgccagctt ttcccccata aagcagatgt ggttggcgag gtgattctgg tcggcaatat
     4141 gcccgccaga gtcattggtg tggcggaaga aaaacagtcg atgtttggta gcagtaaagt
     4201 gctgcgtgtc tggctacctt acagcacgat gtccgggcga gttatgggcc agtcgtggct
     4261 gaactccatt actgtcaggg taaaagaagg atttgacagc gccgagacgg aacagcaact
     4321 cacgcgttta ctttcactgc gccacggaaa gaaggatttc tttacctgga acatggacgg
     4381 cgtcttgaaa actgttgaaa agaccacacg tactttacaa ctgtttctga cgctggtggc
     4441 ggtgatttcg ctggtggtgg gcggtattgg tgtaatgaat attatgctgg tgtcagtgat
     4501 cgagcggacg cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt
     4561 gcaacagttc ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt tgggaataac
     4621 tctgtcactg ttaattgctt tcaccttgca gcttttctta cccggctggg agattggttt
     4681 ttcaccgttg gcactgctgc tggcgtttct ctgctcgacg gccaccggga ttttatttgg
     4741 atggttaccc gcacgaaatg cggcacgact ggatccagta gatgctctgg cacgagagta
     4801 atttttgaga taaaaatgcc agccgatcgg gctggcattt tgcctttagg atgtacacaa
     4861 tgagacagaa gagctatgcg actgccgctt ctacttcgac gggcacaata acactggcgt
     4921 gattgccttt tggcccctgg tggacatcaa actgaacgga ttgtccagct tttagcgttc
     4981 tgtaaccatc catctgaatg gtggaataat gagcgaaaat atcttcgccg ccgccttcag
     5041 ggcagatgaa accaaaccct ttggcattgt tgaaccactt aacagtaccc ttttccatgc
     5101 ttcgacatcc ttcgcaaatc ttatacaagt aagatggaat aaaccggggt cagagagggg
     5161 gctgttcaaa acctcgccaa ctctagaaat acaatttaga gaattagggc gagccgtcaa
     5221 gcgtttgaca ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt
     5281 gacaggaatg tgacagatgt cgctgatgcc aacgatagat gatagttatc tatcatgtgg
     5341 agtagattgg tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc gataaccgta
     5401 accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg
     5461 cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca
     5521 atgatgatta cactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg
     5581 atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgcg
     5641 gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg
     5701 agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg
     5761 gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag
     5821 cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta
     5881 acccatctgc ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg ctccgtcagg
     5941 aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg
     6001 acacacagcc gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc catgtccaat
     6061 cctccggtcg caatgaggta accggtgcaa acgttctggt cgctatcttt agcgaacagg
     6121 agtcgcaggc ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat gtggtgaact
     6181 ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc
     6241 caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga
     6301 atcagcttgc gcgcgtgggc ggaatcgacc cactgattgg tcgtgagaag gagttggagc
     6361 gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctggtg ggggaatctg
     6421 gtgtcggtaa aaccgcgatt gcggaaggtc ttgcctggcg aattgttcag ggcgatgtgc
     6481 cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca
     6541 ctaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg
     6601 acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg ggggcagcgt
     6661 ctggtggcca ggtcgatgcg gctaacctga tcaaaccgtt gctctccagc ggtaaaattc
     6721 gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc
     6781 tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc gatcgaagaa actgttcaaa
     6841 tcatcaatgg cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag
     6901 cggtgcgtgc agcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata
     6961 aagccattga cgttatcgac gaagcgggcg ctcgcgcacg cctgatgccg gtaagcaaac
     7021 gcaagaaaac cgttaatgtg gcggatattg agtccgtggt ggcccgtatt gcgcgcattc
     7081 cagagaagag tgtttctcag agtgaccgcg ataccctgaa aaacctcggc gatcgcctga
     7141 aaatgctggt cttcggtcag gataaagcca ttgaggcgct gactgaagcc attaagatgg
     7201 cgcgtgcagg tttaggtcac gaacataaac cggttggttc gttcctgttt gccggcccta
     7261 ccggggtcgg gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagctgc
     7321 tgcgctttga tatgtccgag tatatggaac gccataccgt cagccgtctg attggtgcgc
     7381 ctccgggata cgttggtttt gatcagggag gtttgctgac tgatgcggtc atcaagcatc
     7441 cacatgcggt gctgttgctg gacgaaatcg agaaagcgca tccggacgtg ttcaatattc
     7501 tgttgcaggt gatggacaac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc
     7561 gtaacgtggt gctggtgatg accaccaacg ccggggtacg tgaaactgag cgtaaatcca
     7621 ttggtcttat ccaccaggat aacagcaccg atgcgatgga ggagatcaag aagatcttta
     7681 caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt tgatcatctg tcaaccgacg
     7741 tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagctg gatcagaaag
     7801 gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgagaaa ggttacgacc
     7861 gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg
     7921 ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc gcgctggata
     7981 aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag
     8041 cgcattaatc tgattgttag gtaggttggt caagtccgta atctcgaaag aggttacgga
     8101 ctttttgttt atggggtgga ggaggttcag accctttttt taatgatgat ggtaagttgt
     8161 tgataattag tgctgcggga aggtaaggat aaaaaagggt gctgcaggag aatgggatgg
     8221 ttttgcttta ttaacaacgg gctaaacgtg tagtatttga gttcactgcc atacaggcag
     8281 cttagaaatc gacggggtgc ggtaaaacct ttgcgaacgc gttcactgcc gtacagacag
     8341 ataaaatgcg aaaaaaaagc tcgcactttc gtacgagctc ttctttaaat atggcggtga
     8401 gggggggatt cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca
     8461 gccactcgga cacctcacca aattgttttg tcgcctgacc tcatgggtgg caacggggcg
     8521 ctactatagg gagttggagt aaaacggtca agaagaattt taatgataat tattgtttgc
     8581 tcatactgta aacaagttgt gcagtatatc tacatcgaga caagttacgg acttatactt
     8641 ccaaagtact tcatacatat cacaaaataa aaaggccggt taaaccgacc ttttactcgt
     8701 tctttctctt cgcccatcag gcggtaaaac aatcagcgac tacggaagac aatgcggcct
     8761 ttgctcaggt cgtacggggt cagttcaaca gtcactttgt cgcccgtcag gatgcggatg
     8821 tagtttttgc gcattttacc ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct
     8881 acgcggaaca tggtattagg caacgtttca agaacggtac cttgcatttc aatattgtct
     8941 tctttggcca tctaatcctc tggggtatca ctaccgtaat ttgaaccggc aagataatgc
     9001 cgaagttctg taaataagta aagatttgcg cgctaaatcg caacaaacag gttcggcaca
     9061 ttactccgaa aacacacggc taagccgcac caaaagcgca acgtataagg gagcggtgag
     9121 ataaacgatg ggcgttacct gacgcgaaaa attccttatc ggcagcgggg taatgagcgt
     9181 aaccaactct gcgaccgcaa ttataacact ctggggagaa atgtgccgaa aacattcatt
     9241 cttgtggtga aaacaagcat cgtggtaccc agaaattatt cggcaatcgt ccgaggcgca
     9301 tttgattgag ataattaagg taatcccggc ggggaatttc gcaggcacca agcgatgctg
     9361 tgtgatcgtt aaggacctgg cagtcgataa gcttaccgcc atgaccgata aattcctcac
     9421 agaataccag aagcgccgtt ttagacgcat tttccatccg gctgaacatg gactcgccac
     9481 aaaatagcgt tccctgggcc acgccgtaca taccgccgac aagctcatct tcacgccaga
     9541 cttcaatgga gtgggcatgg ccgagttcgt gaaggcgatg gtaggcttcg accacgccac
     9601 gcgtgatcca ggttccttct tcgcgatcgc tggcacagcc ttcaatgacc tgaccaaaag
     9661 cgtaattcat cgtgacacga tagggcgagc gtttatgaaa tcgcttcata ctacggctga
     9721 tatgcagtga ttctggccat agcaccgcgc ggggatcggg cgaccaccag aggatggggt
     9781 cgcctggaga aaaccacgga aaaataccac gctggtaagc cattaacagg cgcgcagggc
     9841 taagatcgcc cccaagtgcc agcaggccgt taggctcacg taatgcgcct tccggggaag
     9901 ggaaggctat tgaatggcga gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa
     9961 gtcggatcgt tcaataatag cttacaaacc ctgcttgaac tggtaataac gcccctgtct
    10021 ggcaagcagc tctgcgtgag taccttgctc aataatttgc ccgttgtcca tcactattat
    10081 ttgttggaaa cgagagagtc cgcgaagtcg atgggtgacc attaacaccg ttttctcacg
    10141 catcatttct gcaagcaatt caaggatctg gctttcggtt gtggcatcta agccttcggt
    10201 aggttcatcc agcaacacca gtggcgcatc atgtaacagc gcacgggcga tagccagacg
    10261 gcgcagttca ccaccggaga gctggcgtcc gccttcacct aaccaactgt tgagacctgc
    10321 atcctcgagc agcttctcaa ggccaacgcg acgcaagctc tccgccagag cctcatcact
    10381 actgccaggc gaggcgagta acagattatc acgcagcgtg gcgctaaaca gatgcactcg
    10441 ctgaggaaca acgctgatgg tctgtcgtag agccgcttca ttcaggctgg ctatggggct
    10501 atcgttaagc aaaatctcgc cctgttgcgg gtcccaagcg cgggtcagca gttgtaacag
    10561 tgttgatttg ccgcatccgg ttcgcccgag aatcgcaata tgttccccgg cgtttacctg
    10621 aagagaaatc cctttaagtg cctgttggga ttgctccgga taagtgaact gaacatcccg
    10681 taatgtcagc gaaacgcgat cggcaacacg agtttgggta tcagggaagg tgacttccgg
    10741 tttttgatcc gttaaatcag tgatccgaac ggcagaggca atgacttgcc ccagatgctg
    10801 aaatgcaccc gttactggtg ccagtgcttc aaacgcggct aacgcgcaga agacaaacag
    10861 ggcaattaac gcgccgggtt gagcattgcc gccaacgccg ccagacgcca tccacagcat
    10921 caggatcacc gctaacgcgc caatgagtag cattatcgct tgcgacaatg cggtcagttc
    10981 agattgacgg cgttgcgctt ccagccattg aatttctgta ttctctagtt gcgtgcgata
    11041 acgatcgctg gcaccaaaaa tggtcagctc agcttgccct tgcagccagg ccgtcagttg
    11101 ttggcgatac tgtccgcgaa gatgagtcag attttgcccg gtgctttttc ccgcacgata
    11161 aaacagcggt ggcatcagga aaagcgtcag taacataatg ccgcccagcg taaaggcgag
    11221 ggtgaaatca aggaaactta acccgattgt caccaccata atcaccacaa aagcgcccac
    11281 cagcggcgag ataacgcgca gataaagatg atcgagcgta tcaacatccg ccaccacgcg
    11341 attgagcaat tcgccctgac gatagcgcgc cagtccggca ggggagaggg gcagcaattt
    11401 gctgaaggtg taaatgcgca gatgctgcaa cacgcggaaa gtcgcgtcgt gacttaccag
    11461 acgttcaaaa taacgcccgg cagtacgggt gattgctgcg ccacgcacgc ccgcagcggg
    11521 tagcatatag ttgaagctgt acagtccggc aacccccgca accgctgagg ccgagaggaa
    11581 ccagccggaa agtgtcaaca gaccgatact ggcgagcagc gtgacaattg ccagcacgat
    11641 accaagactt aacatccatt tatgacgttt atacagtgcc agatagggta gcaaagcgcg
    11701 catttaaatc tcctcctgac gatgggccag taatgtggcg aatgggccac cagccacact
    11761 taattccgcg taacgtcctt gctcaataat ccggccatcc tgcataaccc aaatgacatc
    11821 ccagtcagca agatcttcta actggtgggt gaccattaac gttgtctggc gcagagaggc
    11881 ggcattcagc gcctccatta cgcgctgttc actgtgagca tcaaggctgg cagcgggttc
    11941 atccaacagt aatagcgaac agggatttag taacgcacgg gccaccgcca cgcgctgcgc
    12001 ctgccccacg gaaaggcggg cagcctggtc gccaacaggc gtatcaacgc cttgtggcag
    12061 gagcggtaga aactcgctga cccaggcgtt atccagcgct gcttgtaatt cttgttcgct
    12121 ggcatcaggt cgcgccagta gtacgttatc ccgcaatgtt gctgccggta attgtgggtt
    12181 ttgcccaacc caggagagat gtttacgcca tgattctggt gataaatcgc gtaattctat
    12241 cccgttgatt cgtaatgatc cctgatatga aagaaaacca gaaagcgcgt tcagcagtga
    12301 gcttttacct gaaccgctgc gaccaaccaa cactgcacgt tggcctgctg gcaaagtaaa
    12361 gttcagcggt ccggccagcg ttttaccttc cggcgatgtg ataaacagat cctcggcctc
    12421 aatggtcacc ggatcggtcg atgctaattc cgcctcaccg cgctgcggat gggcgagcgg
    12481 ggtttccata aacgttttca gactgtcagc tgcaccaaca gcctgggctt tagcatgata
    12541 aaacgtaccg agatcgcgca atggctggaa aaactctggc gcaaggatca gggccagaaa
    12601 acccgcagcc agcgtcacac cggtatcgta gtgaccaaaa tccagctcgc cgagatagga
    12661 aaaaccaaag tagaccgcca ccagagcaat tgacagcgag gtaaaaaatt cgagaatgcc
    12721 ggaggataaa aacgccagcc gtagcacttc cattgtccgt tggcggaaat cttccgaagc
    12781 agaacgaata ctttcaattt cagcttcacc acgaccaaaa atacgcaatg tttccatgcc
    12841 gcgcaggcga tcgaggaaat gcccacttaa gcgagcaaga gcgagaaagt tacgccggtt
    12901 agcatcggca gcccccatcc caaccagcgc cataaacaac ggaattaacg gtgctgtgcc
    12961 cagcagaatg agcgccgcag cccagttaga ggggaagatc gccaccacaa tcagcaacgg
    13021 caccgacact gccagcgcca tttgcggcag gtagcgtgca tagtaatcat gcatatcgtc
    13081 aatttgctcg agtaccaacg tcgcccagct ccccgcaggt ttaccctgaa tccacgctgg
    13141 ccctgcttgt tgcagacggt cgagaacctg acggcggatg gcaaagcgga tatgctgccc
    13201 ggcgtgataa cccacccgtt cgcgtaacca gaccacccat gcgcgcagta caaaggtcag
    13261 aaccagtaac gtaaagggaa gcagcagggc ttcacgggga atattctcca taatcatatg
    13321 ttgcagaatt cgcgccatga accaggcctg ggcaataatc aatatgccgc ttacaaagcc
    13381 cagcagacga gaaatattca gccaacgttg ggagatgacg ctttgctgtt ttaaccagcg
    13441 ggttaactct ttttgacggg atttattcat tgcacgctta gcaggtgagt tatcagaatt
    13501 atttgcagag caatgttaca acggggaaaa aataaaggcg acccatagtc gcatggtgtc
    13561 gccttcttta cttttgttac tgatttgtaa aattattttg cgtcagctaa accatcgagg
    13621 tagcgttccg catcaagtgc tgccatgcag cctgtaccgg ccgaagtaat ggcctgacga
    13681 taaatatgat ccatcacgtc acccgcggca aagacgccag ggatgctggt ctgggtggca
    13741 ttaccatgaa tacccgactg cactttgatg tagccgtttt ccagttccag ctgcccttcg
    13801 aaaatggcag tattcgggct gtgaccgata gcaacaaaca gaccagcaac gtcgagtgac
    13861 tcgatgttat cgctgttttg cgtatcgcgc agacgaacgc cagtgacacc catttgatcg
    13921 ccggtcactt cttccagcgt acggttggtg tgcagaatga tgttgccgtt ctccacttta
    13981 tccatcagac gcttaataag gattttttcc gcgcggaaac cgtcacggcg gtgaatcaga
    14041 tgcacttccg aagcgatgtt agacagatac agcgcctctt caaccgcggt attgccgccg
    14101 ccgatgaccg caactttctg gttgcgatag aagaaaccgt cgcaggttgc acaagcagaa
    14161 accccacggc ctttaaaggc ttcttcagag ggcaggccga gatagcgtgc agaagctccg
    14221 gtggcaataa tcagcgcgtc gcaagtgtat tcgccgttat cgccattcag acggaacgga
    14281 cggttttgca gatccacctt gttgatatga tcaaaaatga tctcagtttc aaacttggtg
    14341 gcatgttcgt gcatgcgctc cattaataac ggaccggtca gatcgtttgg atcgccaggc
    14401 cagttttcca cttccgtggt ggtggtcagt tggccgcctt tttccatgcc ggtaatcagc
    14461 acaggttgca ggttggcgcg cgccgcgtag acagcagcgg tgtatcccgc cgggcctgaa
    14521 cccaggataa gcagtttact gtgtttggtc gtgcccatga gatccccata gttgttggca
    14581 gacaatgggc aggattgtag ggaatttaca gacgtaaaaa aagagtatga cgattttgtt
    14641 aacaatttgt gcaatcggca gcatcgataa gcaggtcaaa ttctcccgtc attatcacct
    14701 ctgctactta aatttcccgc tttataagcc gattaaatga tgaataaacg cccctgttaa
    14761 tgaatatctg gcatgttgta ctaaaaatcg atgttttgct ttgacaatcc cctggtgttt
    14821 tgcgaaaaca ttcgaggaag aaaaaaaaca gtattcttat atgcgcataa ccatgcatgt
    14881 aaataccatg tttaccgtgc tagtgaaatc tacgtatggc gtggacagac gccattcgtg
    14941 atgtcgatag ctgccacaag gcaacggtct tctcaccgta gacccaggca ttgcgcgccg
    15001 tgaatcttca tgatttcggt ctatcgtgac gggtagcgac tctgaacagt gatgtttcag
    15061 ggtcagacag gagtagggaa ggaatacaga gagacaataa taatggtaga tagcaagaag
    15121 cgccctggca aagatctcga ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat
    15181 gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac tttccccaac gccgtgcctt
    15241 gagcgtgtgc gtcggctgga aagacaaggg tttattcagg gctatacggc gctgcttaac
    15301 ccccattatc tggatgcatc acttctggta ttcgttgaga ttactctgaa tcgtggcgca
    15361 ccggatgtgt ttgaacaatt caataccgct gtacaaaaac ttgaagaaat tcaggagtgt
    15421 catttagtat ctggtgattt cgactacctg ttgaaaacac gcgtgccgga tatgtcagct
    15481 taccgtaagt tgctggggga aaccctgctg cgtctgcctg gcgtcaatga cacacggaca
    15541 tacgttgtta tggaagaagt caagcagagt aatcgtctgg ttattaagac gcgctaacac
    15601 ggaacaggtg caaaatcggc gtattttgat tacactcctg ttaatccata cagcaacagt
    15661 actggggtaa cctggtactg ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg
    15721 gagagccttt cttgagccag gaatacactg aagacaaaga agtcacattg acaaagttaa
    15781 gtagcggccg tcgccttctg gaagcgttgc tgatccttat tgtcctgttt gccgtctggt
    15841 tgatggctgc cttactaagc tttaaccctt cggaccccag ctggtcgcaa acggcctggc
    15901 atgaacctat ccataattta ggtgggatgc ccggtgcgtg gctggcagat acgctgttct
    15961 ttatttttgg cgtgatggct tacaccattc ccgtcattat tgtcggcggt tgttggtttg
    16021 cctggcgtca tcagtccagt gacgaataca ttgattattt tgccgtttcg ctacgcatca
    16081 ttggcgtttt ggcgctcatc cttacctcct gtggtctggc ggcaatcaac gctgacgata
    16141 tctggtattt tgcctccggt ggcgtcattg gcagcttact aagcactacg ctacaaccac
    16201 tgctacacag tagcggggga actattgcgc tgctctgcgt ttgggctgcg ggcctgacgc
    16261 tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact cggcggctgg attttaaaca
    16321 ttctcacctt cgccagtaat cgtacccgtc gcgatgatac ctgggtcgat gaagatgaat
    16381 atgaagatga cgaagagtat gaagatgaaa accacggcaa acagcatgaa tcgcgccgtg
    16441 cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc
    16501 cgatggggcg gcaaacagac gctgcgttgt tctccggtaa gcggatggat gatgacgaag
    16561 agattatcta cactgcacgc ggtgtggctg ccgacccgga cgacgtccta ttttcgggca
    16621 atcgtgcaac gcagccagaa tatgacgaat acgatccatt attaaacggt gcgccaatta
    16681 ccgaacctgt cgctgtagca gttgctgcta ccacggcgac acaaagctgg gctgcgccgg
    16741 ttgaacctgt gactcagacg ccgcctgttg cctctgttga tgttccacct tcgcaaccta
    16801 cagtagcctg gcagcctgta ccgggtccac aaacgggaga gccggttatt gctcctgcac
    16861 cggaaggtta cccacagcag tcacaatatg cgcagcctgc agtgcaatat aatgagccgc
    16921 tgcaacaacc agtacagccg cagcagccgt attatgcacc tgcagctgaa caacctgcgc
    16981 aacaacctta ctacgctcct gcggcggaac aacctgttca gcaaccgtat tacgcccctg
    17041 cgccagaaca accggtggca ggtaacgcct ggcaagccga agagcagcaa tccacttttg
    17101 ctccacagtc tacataccag actgagcaaa cttatcagca accagccgct caggagccgt
    17161 tgtaccaaca gccgcaatcc gttgaacagc agcctgttgt ggagcctgaa cccgttgtag
    17221 aagagacaaa acccgcgcgt ccgccgcttt actattttga agaagtggaa gagaagcgag
    17281 cccgtgaacg tgaacaactt gcggcctggt atcaaccgat tccagaaccg gttaaagaac
    17341 cagaaccgat caaatcttcg ttgaaagcgc cttctgttgc agcagtacct ccagtagaag
    17401 ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa agcgacactg gcgacggggg
    17461 ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa tagcggtgga ccgcgtccgc
    17521 aagtcaaaga ggggattggc ccgcagttgc cacgaccgaa acgtatccgc gtgccaactc
    17581 gtcgtgaact ggcgtcttac ggtattaagc tgccctcaca gcgtgcggcg gaagagaaag
    17641 cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca gtataacgat gatgaaatcg
    17701 atgcgatgca gcaggatgaa ctggcacgtc agttcgccca gacacagcag caacgctatg
    17761 gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga tgcagatgct gcggcagagg
    17821 ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg ttattccggc gaacaaccgg
    17881 ctggggcgaa tccgttctcg ctggatgatt ttgaattttc gccaatgaaa gcgttgctgg
    17941 atgatggtcc acacgaaccg ttgtttacgc caattgttga acctgtacag cagccgcaac
    18001 aaccggttgc accgcagcag cagtatcagc agccgcaaca accagttccg ccacagcagc
    18061 aatatcagca gccgcaacag ccggttgcgc cgcagccgca gtatcagcag ccacaacagc
    18121 aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc ggttgcgcca cagccgcaat
    18181 atcagcagcc gcaacaacca gttgcgccgc agccgcagta tcaacagccg caacaaccgg
    18241 ttgcaccgca gcagcaggat accctgcttc atccgctgtt gatgcgtaat ggcgacagcc
    18301 gtccgttgca taaaccgacg acgccgctgc cttctctgga tttgctgaca ccgccgccga
    18361 gcgaagtgga gccggtagat acctttgcgc ttgaacaaat ggctcgcctg gtggaagcgc
    18421 gtctggctga tttccgtatt aaagccgatg tcgtcaatta ctctccgggg ccggttatca
    18481 ctcgctttga acttaacctg gctcctggcg taaaagcggc gcgcatttct aacttgtcac
    18541 gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca
    18601 aaccctatgt aggtctggag ttaccaaata aaaaacgaca aaccgtttat ctgcgcgaag
    18661 ttttggataa cgccaaattc cgcga
//
LOCUS       DAHBHC010000096        18577 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHBHC010000096 DAHBHC010000000
VERSION     DAHBHC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.097"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(138..1007)
                     /locus_tag="M2A35_004146"
     CDS             complement(138..1007)
                     /locus_tag="M2A35_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCB7200370.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLERAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="M2A35_004147"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="M2A35_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCB7200371.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLTREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="M2A35_004148"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="M2A35_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCB7200372.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="M2A35_004149"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="M2A35_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCB7200373.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="M2A35_004150"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="M2A35_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCB7200374.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="M2A35_004151"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="M2A35_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCB7200375.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="M2A35_004152"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="M2A35_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCB7200376.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="M2A35_004153"
     CDS             complement(4585..5718)
                     /locus_tag="M2A35_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCB7200377.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="M2A35_004154"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="M2A35_004154"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCB7200378.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="M2A35_004155"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="M2A35_004155"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCB7200379.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="M2A35_004156"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="M2A35_004156"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCB7200380.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="M2A35_004157"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="M2A35_004157"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCB7200381.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="M2A35_004158"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="M2A35_004158"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCB7200382.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="M2A35_004159"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="M2A35_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCB7200383.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(12266..15589)
                     /gene="mscM"
                     /locus_tag="M2A35_004160"
     CDS             complement(12266..15589)
                     /gene="mscM"
                     /locus_tag="M2A35_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCB7200384.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDSE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(15611..16579)
                     /gene="psd"
                     /locus_tag="M2A35_004161"
     CDS             complement(15611..16579)
                     /gene="psd"
                     /locus_tag="M2A35_004161"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCB7200385.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16676..17728)
                     /gene="rsgA"
                     /locus_tag="M2A35_004162"
     CDS             complement(16676..17728)
                     /gene="rsgA"
                     /locus_tag="M2A35_004162"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCB7200386.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNISYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17823..18368
                     /gene="orn"
                     /locus_tag="M2A35_004163"
     CDS             17823..18368
                     /gene="orn"
                     /locus_tag="M2A35_004163"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCB7200387.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4301 a         4759 c         4837 g         4680 t
ORIGIN      
        1 ctggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg tgggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgctccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttttta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tgctcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttagcta tataaaaagt aacaacagca atgcatatga ataattaaag
     1201 gttatgcaac gtgcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgtc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gccctgttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc tatcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc
     4981 catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac
     5041 atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt agaacccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaagtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 acgccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggtg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgcc ggactggagc ctacgcaggt ggttggtctg ctggcagtag catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 tgtcagcaca aactggcagc aggtattaag taatggttcc gttaacctcg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggtg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggg gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccttctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attctttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcgtatgtc ggcaatgatt gcgacggtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ccggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagtc gcatggcgaa taaacctgag ttagctctcg actgatcata
    12121 tgaaatcctg caactcatcg tctctccggc gatgagttgt caggaagtaa tgcgtatgga
    12181 tgatactctc cagggccggt gataactgaa gatgatgtta agctgctaaa tgagccacgt
    12241 tgacatcatt tggcagcttc atgcattaca aacttcccgc ctgacgacct ttgcccgcag
    12301 aagttaatgt tctccccgtt tgcttaccgt tgaggctttc cagacgcatc tggaagggcg
    12361 ggaatggcat atcgataccg tgggcgtgga agcctgccag aatcagctgg tggatctcat
    12421 ggcgtagcgg catacggtga cccatctcag cggcgtaaat acgcagctcg aaaatctgaa
    12481 tcccctgttg cagatccacc aggaagactt ccggtgccgg gttgtcgatc accagcgagc
    12541 agcgacgcgc tgcggtgagc aggatttccg tcacttcttc gctattggca tcggcagggg
    12601 ccggtatcgt caacaccacg cgcgtgaccg agtcagagag cgaccagttg ataaactgct
    12661 cggtaataaa cgccttgttc ggcacgatta tctctttacg gtcccagtcg ctgatggtgg
    12721 tggcgcgggt gttaattttc gtcacgctac cggtaagatc gcggatcgtt accgtatcgc
    12781 caatgcgaat cggtttttcg aacaggataa tcaggccaga gataaagttg gcgaaaatct
    12841 cctgcaaacc aaaaccgaga ccaacaccga gcgcggcaac cagccactgc aatttcgacc
    12901 actcaatacc aatcatcgag aagccgacca gcccgccaat cagcatcagc agatatttgg
    12961 tgatggtggt gatggcgtag cccgtacccg gcgttaaatc caggtgctgc aaaatcgcca
    13021 gttccagcag cgcgggcagg ttgcgcacca gctgcgtggt gatgataaac accagaatgg
    13081 caatcagcac cgcaccgagg gtaattggct ccagactttc tacgccctgt accgtggagg
    13141 tgacatccca cagcgaaata ttttcgagga agccgaaagc ggaatggatt tctgaccaca
    13201 gcacaatgac agaaagcagg gcgatcaaca tcaaaattga gcgcaccagc cgcaaggatt
    13261 gcgcactgat ggcatcgaga tcgacttcgc tttcatcgac ttcaattgcc ccttccgggc
    13321 tactgtgatg atgcgcttcc tcttcgccac gcgcgcgttg cgctaacatc tctgcccggc
    13381 gatgcttcgc ccggtcaaac gccagcctgc gacgctggat cagcatccag cggcggataa
    13441 cgtgataaac caccagtagc aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca
    13501 gtgcctgcgc cgttgccaga taacccaccg ctgacgccag aatggcaacc agtggtgcgc
    13561 caatcatcat gttccacagc atatggttgg taatgttgtc gccgctgccc tctttgttga
    13621 ggtacagcgg gatcccggcc tttttcaggc tgagggtgac caccgccagc gcaccgcaaa
    13681 tgaggataaa gcaaagccgt cccagcgaac cggagaattc actgtcgtcg aggttatcga
    13741 acatcatcag cgccataatc agcggcacaa taagcccgat gctcatcagg tagtagcgca
    13801 tcccacggga aacacgttcg cgcggccagc caaaatgagc gataaacaag ccgttcgggc
    13861 gggcaaaggt ggcgcaaatc atcactaccc acagcagcgg cacggtggcc gttacgccat
    13921 cgccaatcgc gaccgccagc ggataaggcc acgcctcgcg caagccgtaa cccagcgtca
    13981 tccacagcac cggcagcggt gacgcgacga gaatcgacca gaaaagagtg cgcaacgtca
    14041 gccagaagtg atcctgagtc actttgccga ctttcgccgc cgaacgttca agaaaacggg
    14101 tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat caacgcgcca aacagcggca
    14161 aaatcgtctc tttgctggtc agcatcatca cactggcttt gcccaactga ctgaaggtgt
    14221 ccagcgaaat gagacgacgc agatcctggg cgatttccag cggccaggcg atggtcatcg
    14281 ggcgcacgtc agaggtccag aacagatagc ggtgcgttgc ttcgttcacc tctttcagcg
    14341 catcctccag ttgcccgttg gagactttca gcttggtcag ttccagtagt agcgtgtcgc
    14401 caccctgcaa caatgagttc agcaactcac gctgagtgcg cagttgtgct tccagaatac
    14461 ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg aatttgccgt agcagcggct
    14521 gtttattaag cagatcctca taacgtaacc gttgcacacg caactgcgcc atttcggtat
    14581 caagctgttg tggtttcggc atttccggca gccgtgccac ctgcgcccgc aacgcttcgc
    14641 cgagcaggtt ggacgatccc agccattgcg actgttcacg cagcgtattc agcgcctgcc
    14701 gaacctgtaa cgtctggctg gtggcctggc gctgttgcga ggcaacgaga tccatccgct
    14761 gcgcctgttg attcagtgcc gccgatagtt cgcggttaat tttgaattgc gcgacgatgt
    14821 ctttcggcaa atcggcgctg ttttctgcca gcaattcggt actttccagc gcccgctccg
    14881 cctcaagctg acgttggctg ttcaactgat tacgtaaggc ctgcaaatac gcatccagtt
    14941 gctggctctc tttttccgcc agctctgagc gtaagcgcgc taattcctga cggttattgg
    15001 cagacagctg cgccagttcc agttcatcaa cgagcgcttt caggcgtgcg gagtcagact
    15061 gcaacgcgaa attttgtgcc tgattgagcg gggtattgcc agtaagcgtt cccaggcggc
    15121 gctcgatctc atttaactga cggcgggcgt cggtttgctg ttgcggcagt tgattcagcg
    15181 aatcggcaat ctcgcgggcg cgctcctgct cttgctgggc ctgacggctt ttatccagca
    15241 actggctgct gacctggaga atttcctgat tcagcgcgtc ggtagacatt cccggcgaca
    15301 cgctgcgcgg ctcgtcacgc atgttgttta attgtgcgcg cagagtagcg gagagtttcg
    15361 gataattatc gataacttgc tgatattgtt tgatgcgctc aagggaacct tttcgttcct
    15421 caagcgcatt taaggcagac tggagcgcct ctacgacttc cggctgtgcg ggtttcgccg
    15481 cttttgcctg ctccagttcc tgagtgattt gtttgctatc gggggccgtc gcggcgtacg
    15541 ccccccaact gaggcaccag gccatcagaa aagtgataat caggcgcacg tcagcgtttc
    15601 ctttgatgga ttagacctgg tcttttttat cgtcaaccaa tgggctggcg tcgtgttctg
    15661 cttcgatctc ttcagcagga agtggggcgg gttcagcgtc tggcgtaaca aaggtttcgg
    15721 tagatactgc cagcggctgg ccaattttcg tgacagacag gctttccagt tgctcaacca
    15781 gattcacttt acccggtgca aacaggttga taacggtaga gccgagttta aagcggccca
    15841 tttcctggcc tttcagcagt gccacagaac cgtcgttttc cccggcaggc caggtccagc
    15901 gcttgatgat accttcgcgc ggcggcgtaa tggtgcccgc ccagaccgtc tcaatgctgc
    15961 ctacaatcgt cgccccgacc agaatctgcg ccatcgggcc aaattcggta tcgaacaggc
    16021 aaatcacgcg ttcgttacgg gcaaacagat tcggcacgtt ctgagcggtg agatggttaa
    16081 cggagaagag atcgcccggc acgtagatca tctcacgcag aataccgttg cacggcatgt
    16141 gtacgcgatg gtagtcacgc ggggagaggt aagtggtcac aaacgtaccg ttgcggaaca
    16201 ggtccgccat cagatagttg cctgccagca gggcttcgag gctgtagttg tggcctttgg
    16261 cttgcaggat tttattttct tcgattttac ccaactggct gataacgcca tcggcaggca
    16321 tgaccaggac attcggatcg gtatcgattg ggcgtacttc gtcacgcagc ggacggacaa
    16381 agaattcgtt aaaggtgcgg tagctggcgg tgtccggctt ttgcgcctct ttcatgtcga
    16441 ccttgtagta tttaacgaac agatcgataa ccagttttgt cagccatcct gcccgcttgc
    16501 ttgcgcccca acccgccagg cgagtaagcc atagtttcgg cagaatgtac tgtagcgaaa
    16561 gtttaaatga atttaacaag gtagcctcca ggccattgtt ttgtcgttcc tgatccggcc
    16621 aacatgccgg atcctgaaaa aaaggggacg attctaacga cggttagctt aattgtcagt
    16681 catccgtatc agaaaagttt ttacgcgttt ttacctgcgc catgctttcc agaatacggt
    16741 gatagttttc gaaacgggtt tccgcgattt tcccttcctc aaccgcttcc cggatagcgc
    16801 agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca cagacctaaa tagtcatgga
    16861 attcgacaaa gccctgagtg atttgttccg gctccaggtg ccagaggccg aactcacgca
    16921 caccagggga atcaatcaca tcaccgccgt gcgggaagtg atacagccga gcggcggtcg
    16981 tggtgtgctg accgagcccc gagttgtcag agacatcgtt ggtcaggatc tctttttgca
    17041 gccccagcaa cgcattcagc aggctggatt tgccgacgcc agactgcccg gcaaaaatgc
    17101 tgatgcgccc ggtcaacgcc tcttccagcg gttttagccc atcctgagta tggctggaaa
    17161 ccatcaatac gcgataactg atattgcggt agatatccat ctgctcgttg acgaacgcca
    17221 tgccttcgtc gtccagcagg tctatcttgt tgagcacaat aatcggctca atctgcaagg
    17281 tttcgcaggc caccaggtaa cggtcgataa tattgagcga cagctccggc aaaatggcgg
    17341 agacaatgac aatctggtcg atgttggcgg caataggttt tacgccgtcg tagaaatccg
    17401 gacgcgtcaa caccgaggta cgctcatgca ccgcttccac gatccctttg acatttacgc
    17461 cttccgccgc cggtttaccc ggacgccaga ctacgcggtc gccggttacc agcgaacgga
    17521 tggtacggcg aatattgcag cggtgaacgt cgccatcggc ggattccaca tcagcgtgca
    17581 taccaaagcg gctgatgacg ataccttcat caggctcgcc aaacagattg tcgtcgtagt
    17641 cgggcttctc cttagacgtt ttaagacgac gctggtgatt ggcgttcacg cggcgctgct
    17701 ggcctttgga gagtttattt ttactcaatc gtacagactc ctggtcgccc ctgatgggca
    17761 aaacatctat gatacacgca attgtggatc aatatagtca ctgtgaatgg gtggaaaata
    17821 gcatgagtgc caatgaaaac aacctgattt ggatcgatct tgagatgacc ggtctggatc
    17881 ccgagcgcga tcgcattatt gagattgcca cgctggtgac cgatgccaac ctgaatattc
    17941 tggcagaagg gccgaccatt gcagtacacc agtctgatga acagctggcg ctgatggatg
    18001 actggaacgt gcgcacccat accgccagcg ggctggtaga gcgcgtgaaa gcgagcacga
    18061 tgggcgatcg ggaagctgaa ctggcaacgc tcgaattttt aaaacagtgg gtgcctgcgg
    18121 gaaaatcgcc gatttgcggt aacagcatcg gtcaggaccg tcgtttcctg tttaaataca
    18181 tgccggagct ggaagcctac ttccactacc gttatctcga tgtcagcacc ctgaaagagc
    18241 tggcgcgccg ctggaagccg gaaattctgg atggttttac caagcagggg acgcatcagg
    18301 cgatggatga tatccgtgaa tcggtggcgg agctggctta ctaccgcgag cattttatca
    18361 agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc acttgcggcg gcgtgatttt
    18421 gacgctaaat tgaacgtctt gctgataatt tcagcacttg agataaaaac gcaaaaaaaa
    18481 cttttttggg gggttgcaga gggaaagatt tctcgtataa tgcgcctccc gtaacgacgc
    18541 agaaatgcga aaattacgaa agcaaaatta agtagta
//
LOCUS       DAHBHC010000097        18563 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHBHC010000097 DAHBHC010000000
VERSION     DAHBHC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.098"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1543)
                     /locus_tag="M2A35_004164"
     CDS             complement(<1..1543)
                     /locus_tag="M2A35_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCB7200388.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAISVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDN"
     gene            complement(1947..2708)
                     /gene="ydeO"
                     /locus_tag="M2A35_004165"
     CDS             complement(1947..2708)
                     /gene="ydeO"
                     /locus_tag="M2A35_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCB7200389.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
     gene            complement(2783..2980)
                     /gene="safA"
                     /locus_tag="M2A35_004166"
     CDS             complement(2783..2980)
                     /gene="safA"
                     /locus_tag="M2A35_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCB7200390.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            complement(3228..5507)
                     /gene="ydeP"
                     /locus_tag="M2A35_004167"
     CDS             complement(3228..5507)
                     /gene="ydeP"
                     /locus_tag="M2A35_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCB7200391.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEPA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(5841..6755)
                     /locus_tag="M2A35_004168"
     CDS             complement(5841..6755)
                     /locus_tag="M2A35_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200392.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            complement(6814..7317)
                     /locus_tag="M2A35_004169"
     CDS             complement(6814..7317)
                     /locus_tag="M2A35_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200393.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(7330..7860)
                     /locus_tag="M2A35_004170"
     CDS             complement(7330..7860)
                     /locus_tag="M2A35_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200394.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(7874..10525)
                     /locus_tag="M2A35_004171"
     CDS             complement(7874..10525)
                     /locus_tag="M2A35_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7200395.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLLDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            complement(10567..11277)
                     /locus_tag="M2A35_004172"
     CDS             complement(10567..11277)
                     /locus_tag="M2A35_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCB7200396.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVLPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(11638..12201)
                     /gene="fimA"
                     /locus_tag="M2A35_004173"
     CDS             complement(11638..12201)
                     /gene="fimA"
                     /locus_tag="M2A35_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCB7200397.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(12633..12841)
                     /locus_tag="M2A35_004174"
                     /pseudo
     CDS             complement(12633..12841)
                     /locus_tag="M2A35_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(13485..14744)
                     /locus_tag="M2A35_004175"
     CDS             complement(13485..14744)
                     /locus_tag="M2A35_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HCB7200398.1"
                     /translation="MKLVTVFFSFVLLSFFSPAYAVSENEAGRVEMSMDNAPLPQVIS
                     MVWQRVFNRPYQLSPDIAGDTRLVSFYLSKNQEPRSFFISYLKRLNISVTSMKDGVDY
                     ISVIKQTEQKVPDVVFTYRPRYRSVSYLSAMLNSVVSSGAFSNHIQSVDYLSGGVSGT
                     STTDSASTRMSMATDAEVLVYSGPAASVRQIEKILPRIDVPAEQVTVSGYVLEVQTTD
                     RNATGLQIIADLFRNKLGMSVGARLDGGNSFTLNVGGLNAFYSLIKEDSRFNVVSNPR
                     LTVLSGSKSQFTVGQEVPVLDSVSYQGSSGTPVQSVTYRNSGAIFTVTPVVLDGLITL
                     DISQQLSDFVKTTTGVNSSPTLTKREISTKVDVKDGELLVLGGLASSKLTQSRTGFSF
                     LPGFTGKSDEDNRTDIIVVLQARRVGK"
     gene            complement(14741..15799)
                     /locus_tag="M2A35_004176"
     CDS             complement(14741..15799)
                     /locus_tag="M2A35_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin"
                     /protein_id="HCB7200399.1"
                     /translation="MAITAYIGVPGSGKSYEVVKSVIIPAVASGRRIVSNIYGLNYEA
                     IVQYCHKNKLISDDVSPGEIIHVENERVMAPDFYPVKGNQDKSLCQPGDLIILDECHR
                     FFTSDKALSSDARIFAAEHRHYADEKTGQTCDLVLINQALTTLPRFLRERIEQTFRMK
                     KLIGLSHKSYRVDIFDGSRTTKATHLSNYVCRYSKDIFPLYSSHDVKGAVETRTDARA
                     VLFKPQVIVLFVFLILLSVYLFFSFLLPFFNPQKKTEQAQQSAPLRSSPSSPSVNVSS
                     LPENKTASAPSQKWCVIGRFNDGDRNYVFLRDTENRLRMVSANKFRGLNIMLEGEVDG
                     VKVVAWSCNNPVSVRGQK"
     gene            complement(15811..16098)
                     /locus_tag="M2A35_004177"
     CDS             complement(15811..16098)
                     /locus_tag="M2A35_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HCB7200400.1"
                     /translation="MFAIFIAAINTALGFIFRTALIKFVMFSAIYIMVAEVLPLIVTH
                     LPDGSSISSLFSELPSSVLWFLNLMSFDVVLPMIVSAMFTRFFIRRIPFFN"
     gene            complement(16115..17536)
                     /locus_tag="M2A35_004178"
     CDS             complement(16115..17536)
                     /locus_tag="M2A35_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200401.1"
                     /translation="MTWSTLAYGLGVTTVSIGIYAVGKPFAEEIAKLALEKNGYNAFF
                     STPAMSSGSKVWIGGLSSCGVATSTSPFSTASQLLNCLPADSSPSLTRSYYTPKKTIG
                     SLEGYTFYYISKYNGRWSESSLYVYSSVLGSDSDTYCDQGQVLFTPSGGSSSCIAAVT
                     ENPFSSEYSTPSVSGNNLASQDIADILNGLTKQLDGLSSYDGVSSSSLYFTASDVDNY
                     RSTVDMSGGDSGGFTGTGEIAIPPTPEGDTESSGGKYTPPVVDPDFTPVSGNGSSGSS
                     GGTGSGTGTGTGTGSGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
                     TGTGTGTGTGTGTGTGTGTITGVGTGSASTTVTGDATVNVNVTVTNDFGTAPDVSTPD
                     ISSPEPSSFISPLWDMWPSARDFSLTIPRGQCPVFTFNIWNKDYQLDTFCVLLDTDEV
                     RATFRVIMTLIGSMLSFFIVLRS"
     gene            complement(18098..18349)
                     /locus_tag="M2A35_004179"
     CDS             complement(18098..18349)
                     /locus_tag="M2A35_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200402.1"
                     /translation="MFAKKTDVFKGANEKVAALSFSAASALAASPSFAESTVDFSSLT
                     SSIDMSSVSAVILSIGVAGVTVYLTFAGVRKVWGAIRSI"
BASE COUNT         5357 a         4223 c         3942 g         5041 t
ORIGIN      
        1 ggttatcttt ttgctgtaga tccgtcagtt tgtagagacc taactgattg ttttctactg
       61 tatagacaag cgaataatcg ttatttctca ccgtataaga gaattggctt aagtcctcag
      121 tgttggggtt atgcgggtaa tcgtctgact gatggcgaac aaatttgtgg taattatccc
      181 agaatggaat attttcctcg tcaaaccagt gagaataaga ggttatccag gtcagatttt
      241 tatgtggctc gccttgtttc ttatcttgca accagggcag caaggaaacg ccatccagct
      301 taaggtcttt tggaatgctg atatcggctg catcaagagc tgtcgggtag aaatccattg
      361 cggaaatcag cttgtcataa ttaccgggtt gaagttttcc tttccaccac ataaacattg
      421 gggtgtgagt accgccagga taggtctgac tcttatagcc tttttgcgcc ccgttcagcg
      481 gcagaggacc atcgataacc gcaccattat cggaggtaaa gagaataatt gtattgtcat
      541 actgtccgtt tttcttcagt tgttcgagaa tgcgttttac accctgatca acagaataaa
      601 cggaagcgta gtagttatct gctgtttgac taccggtatt aaattgcttc tgatattgat
      661 ccggtgcagg attatcattt ggcaggtgcg gagcattata agccaggtaa agcataaaag
      721 gctggtcaag tgttttggca cgatcaacaa cgctaattgc ctcatcggtt aactgatcgc
      781 tgatataacc ttttgcgggg acacgttcac gatttttgaa cagtgaaggg gagttgtaat
      841 atgccgttcc tgcagcgtgg aatcccataa agtaatcaaa gccacggttt tgaggttgcc
      901 attcttccgc agaaaatgtg gtgaagttgt catgatagtc acgcgtttgt ttatcttccg
      961 gtaccggcac attactgatt tttgacaagt gccatttacc tactgctgca gtgtaataac
     1021 catgattctg gaataattca ggcaagaaag tttctgttag cggaatacca tcctgagcat
     1081 cggtattgga atagacacca aagcgggcgg gagctcgacc ggtcattatt gcggcacggg
     1141 aggggccgga aacaccgtgt gccacatagc cgttagtaaa ccgtacgcct tcatccatta
     1201 atgaaaggag cgtcggcgtt gatttttgtg cagcttcaat ggctttatct atccctattt
     1261 tgtaggtatc gacaacttca cgattttcca ttgtttttgg gtcaaaagat cccttatcaa
     1321 aaggaagttg tccataacca agatcatcca tggttagtac gataatattt ggctttcctt
     1381 tggtactgta ttcagtcggc gtaaagtctg agaaagcaac gtttgttttg gttgctttca
     1441 gctttacatc atctgccgca tgagcagcaa atgcagccat accagatgcc agtatcaaag
     1501 atatcgaggt acttacgaca cttttcttta atgcagactt cataaatgtt cctcttctta
     1561 ttgtacttaa ttccgaagta attttacgaa gagaaataag tgggtgtaag tgaagttagt
     1621 cacataaaga gatagcagat ttagctaaaa aaagggaaat aacagtccat aaagcgttga
     1681 cattactttc tgttctacta agtaatttct tgccgataaa caactaatct attgatattt
     1741 aataaattat tgcattttac tgacaaaatg ccgaattgag atcataaata atcatgcaac
     1801 aggttatgca aatgcataaa tatgtgatga atgtcactta tttatttcaa taaatatatc
     1861 acctaaaaac aacgcggggc agggaatgac tgccccattt ttaattttta cgtagggtgc
     1921 gtggttgact actcgttagc aaataatcaa atagctaaag cattcatcgt gttgcccgta
     1981 ttcataccca tgtgactttg acaacggtac tccttagaaa ctctcttcgg actgttgccg
     2041 aaatgtttgc ggaacgcata aataaaataa gatgtactgg cataaccaca ttgttcggca
     2101 attttattga ctgaaccttc tacgcgtatc aaattttttg cgtgctgcat tctggcatct
     2161 aatagaatct gtgagaatgt cgtttgctct tgctttagtt ttttcttcaa caggctttca
     2221 ctgatgtaca ggcagtcaca aatatctttc agcttccatg ggtgcgccag cttcatgttg
     2281 acaatatttc tcactttccc ggaaacggat agcacaccgt tagttaatag tgtaatgaaa
     2341 cctttgcatg cggcaaaaat agacaggcaa gaaaaaagca aaagttctga aaaattatga
     2401 tgattgcgtt cttccgagtt gagataagca atcatttcat taagcaatcc agtcggtaca
     2461 tttgaagcac gtaaaaactt cgcaggaaca ggcatcgtca ctgtctggat gttagtgcat
     2521 tgtagatatt ttttaatggt attttcatta aatccgattt ctttaaaatg ccgctcataa
     2581 ggttcaaaat gtgtacgaac agcgttatct accatcagga tctcgccgtc agagaaggcg
     2641 taatctttat ctaaaatgtt agcgttgaaa gcatgatgaa taaatatgac agaacaaacg
     2701 agcgacattt tatctcctta aaacaataaa gtttttatcg atactttgtt aacatttcag
     2761 atttataatt tgctgtttat tatcagtctt gcaaactatt gataatgaaa tgtgtaagat
     2821 ctcttgcaat gcgacccatt tctgaaatac ccagctgatc aattgccgaa aaaatggcta
     2881 tcaacgtaag ggtcagtaat aagacaacca caattacaga catgatttct ttatagttgt
     2941 ctctttgcgt gattttgttt ttcactgtgg tcgcatgcat ttcaaatatg tttatttagc
     3001 ggataacgtt aaaaatcgtt aatcagtatg cttaatataa caggctggag aaaagaggaa
     3061 ataggactgg cacttcagta ctgagcggag tttcttacag ctgtaggcag aggtttatat
     3121 cttatagtat gtagtttaat ttaatccatg tgagcaaatt gcgcgataat agtcaatgag
     3181 ggattcttct gctgtgcgga tgacagcaga agaaatgaga agaggcatta atttgatggt
     3241 tctaattcaa ccggaatact tttatagcca ggaatgccac ttaatggatc gtggttatcg
     3301 agtgttagca tgtgattcga ttctggaaaa taggtcacca gtgagcggtc agccataggg
     3361 taaatgacca cttttaatct atccatgcgg cgtgagctac gtttaccgtc tggcgtgagc
     3421 gcaataagat taactctttc gccgtttttt acacggcaaa ttttagcttg tttagcactc
     3481 ataaagacca catctcgttg accgaatacc cctcgatagc gatcatccat accataaatc
     3541 gtcgtgttgt actgatcgtg gctgcgtact gtcgccatga ccagcttact gttaaacgct
     3601 gaagagggat cttctaacag ccctttgctg gtaatgaaat tagccttacc tgacggcgtc
     3661 atccagcgcc tttcagcagc tgcatttatc aggtgaaaac caccgggatg acggatgcgt
     3721 tggttatagt cggcgaactc tggcagcaca gcttcaatgt cattgcgaat gcgatcataa
     3781 tcttccacca gatactccca ggctaccacg ctctggggta gtgctgcctg cgcgattccc
     3841 gcgaccactg cacactctga tttcagcatt acaccggcgg gttttaacac gccacgcgag
     3901 gcatgaatca tcgacattga atcctcaacg gttaccgcct gcgcaccgct tttttgcatg
     3961 tcaatctcgc tacgtcccag aaccggcaga atatagctat gccgtgcggt cagcagatga
     4021 gagcggttaa gcttagtggc tacgtgtacc gccagatcta attgcgttaa cggtacagcg
     4081 ctcgcttccc gatctggcat tgccagtgca aagttgcccc ccatgcagat caatgctcga
     4141 gcctgccctg tacatattgc ttgcatgctg gcaattgcag catgtccagg tgcttgaggt
     4201 ggtgtgaagc catagcgctc acccaggcga gccagaaact ctgcagacgg tttctcggtg
     4261 ataccgacgg ttcggtcgcc ctgtacatta gagtggccac gtagtgggca gatacccgca
     4321 ccaggcttgc caatgttacc tttcatcaac agcagattga ccagttgctg tacattctgg
     4381 gtaccatgtt cgtgctgagt gatccccatt ccgtaacaga taatggtgcg ttcggcagcg
     4441 gcatatgcgt cagccagttc ggcgatttgt gtctgactta gtccagaaat acgttcgata
     4501 tctttccact cggaattgag aacgtcacgg cgtagctcgt caaagccgac ggtatgcgtt
     4561 tgaataaatt cgtcatcaag caatgagggc cgacctgcgg cacttgcagc atcatcgcgc
     4621 tcaattaaca ggcgcatcat ccccttgagc aacgccatat cgccaccaat gcgcacgttg
     4681 tagtaggcac tggccaactg agtctcagag ttcgtcagca tttcaaacgg gttttgcggt
     4741 gcggtaaatc gctccaggcc acgttcctgt agaggattga tggcgatcat tttcgctccc
     4801 cgtttcacta aagcgcgcaa cgaagtcagc atgcgagggt ggtttgtacc agggttatgc
     4861 ccaatgcaaa tgactaaatc gcacttctca aagtcttcca gcaacacagt ccctttaccc
     4921 acgccgatac tcgctgccaa acccacactg gtcggttcat ggcacatgtt ggagcagtcg
     4981 ggaaagttat tgctcccgta ttcacgggca aaaagctgat aaagaaaggc aggttcattg
     5041 gaagtgcggc ccgaagtata gaattcaacc tgattgggat cactatagct ttgaaggcgt
     5101 gcgccaattt cgtcgaaagc ttgttgccag cttaatggct tgtaacagtc gctgacggca
     5161 tcatatttca aaggctgagt gagacgtccc gcagcctcaa gctcgtgatc tccccaggta
     5221 agtaatgatt gaaccgtatt ctcagcaaag aaagaggcat ttacctgctt atccgtgact
     5281 tcccaggcga ttgcttttgc gccgttttca caaatgtcga atgacgcact gtgcttagga
     5341 tctggccatg cacaacccgg acagtcaaag ccctctggct tattcatgtc aaacatggca
     5401 ataacatcct ggcgtatatc catctgctta cgtactgcat tcgctacgga tttaacagca
     5461 ccccaaccac ctgcagcacc ctggtaggat tcaatttttt tcttcatcta cttatcctgt
     5521 gtggaaatga attattagga tttgtaatag cgatgatgca cagtacatac aaagggatta
     5581 tcgccaacat ggatatataa gagtagggga ctactctctt tctattttta atctattatc
     5641 gttaaacaat agattaaata cgataagtta agcatcatct ctcatagtgt ttaaattaac
     5701 atatttttca atataaaaaa taagactctg gcttcaattg tgcgcggatt ttcttacagg
     5761 tgtaggataa tagccttcac aaaataatta tccgcgcaat tttcgtgaat ggagcgcata
     5821 gactaagatc aaaatcacaa ttattcatag ataaaagtga caccaatgac tgactggacg
     5881 gttccggctg caaccttgtt tcctgtttgt ccataggttg cgcttaaacc cagaggtact
     5941 ttgcttttat ttactgtccc cagggagaca ttttctcccg tagccagtat ttttccatta
     6001 cgcatcagag agacacccac tcccgatgct tttgttgcat caggtgctgt gttagcaaac
     6061 acttgtcggc tactgtcagt ggtggcaccc gaaagataaa atgagagctt ttgttcgctt
     6121 gagcaatata cgccaagggg tatttctgcg cttccaggaa agtccgggag atccacagtg
     6181 acattacgac tatcaacggt gcagccgcct gttggcataa caacattgtt atttgaaata
     6241 atattccacg taaaattacg cggatttccg cttcctaatg ttgcaatttt atacatatga
     6301 atacgagcaa tgacttcccc cgcttttatg acaactccac cggccgcccc aactggggta
     6361 atatagagtt ttaacggtaa tggcatcggt gtcttatcac caatatccaa tacgttggta
     6421 tttgtcgtca acgggaaggg gtaggtcaca ttattccagt acagcgatcc tttatatgac
     6481 tgtagggagc ctgcgaaagc tgagccttgt accaggttta tatgatcagt gtcgtaccag
     6541 ccgccgtaat cattccagca acttatatgc tgagacaagt ctacaaccag attctggccc
     6601 ggctgtatta cagggtcgag attaacataa acagaggttg tacctgctcc aatacttgac
     6661 ccgccatcga cattacaact gaaggcaaaa actttgcctg ccattaatag aagataaata
     6721 ccgaacagga cctttatact gattgtttta cccatgatat atcctaaggt taaaaattga
     6781 tttaaaaaga aggctaagaa aatcgaatcg acgttattgc caggtgtaga tcacattgat
     6841 tagcgcctcg atcgttccct ggcttgcgtt tccattcacc gtgatagctc ttgccttaag
     6901 tggaaactgt gcattacgag tgatctcatc aacaataacc gttttgctat cgccattttt
     6961 taacgcagca tcctggtcat ccctcagctc tatctgaata ttttcggcag taccttcatt
     7021 tttgtaataa cccgtattgt cagttgaacc tgtcacgatt gccgtcactg cacttgtttc
     7081 aaccggacaa tcggttaatg acaaagtaat attgtgccag ccagatgcag aaccaggttg
     7141 ttgcagattg cgcgtataaa gatccccgag attaacgtta gcttctttgg tttgaatagt
     7201 gcagggttta gcgactaccc gaccattaac agtgatagtg acatcggcgg cctggagagt
     7261 tgctgtgaat aacgcgcaaa acgtagctaa caggaacctc ttgtgaagtc ttttcatatc
     7321 tgactctgat tattgaaatt caagggtaaa ggttgccgaa gccctcacca gtcccggatt
     7381 gacggacttc tgagttgact tcagacgagc ggagtaaggc aaaatattgt tctgttctgg
     7441 taccagtggg atccactgca tcccggcatg aaggtcattc agtttcaccg gacgcatatt
     7501 tgcgtccaat atttctatac ccaaaccgga ggccgtattg cttccttcat ccagtttcaa
     7561 caaagtatta ttttctgcat cctcaatacc gttaaatgca acccgaaccc ccgttgtccc
     7621 tttgctgcat tcacttaacg taatctgaaa agggacgact ggacttgtgc taccggtcgt
     7681 tggaaattgt ctggcactgt ttttttggag atctacggta aaattaagcg aatccgatga
     7741 gactgtgcag ccataatcga ggacgcgccc gctaatttta ataacgctat ctgcggataa
     7801 agcagaatag gtggttaacc ccagacataa accgaggaaa ataatgttat tgtatttcat
     7861 aatctattgt tccttagcga cagattgctg tctgctggtt cagtaaggta ccaggagaaa
     7921 cttcaggaag cttgtactcg acaatacagt ttgagttttt atctttgccc catgaaacct
     7981 gtaatttccc tgactgtgga agtccagtca gataaacctg accgttttct gcgacaatgc
     8041 tgccattttt attctctccg tgtgtgacaa ttgcaccgaa tggaacgctc ttattaccgt
     8101 acttcaacgt cattaatact ttcccgccga tttgtgcatt aaatgttgct ctggcaatag
     8161 caccgtgagt tgggatgaca gtgaccacgg tttcatccag ttcaacatta tctgcaaggg
     8221 aattcgcgtt aagagcaaca cggttttctc tatattctgt cgcaaatggt aatatggcat
     8281 agccacgcca gtcggtatga attccggtct ggttctctat tttgacatta tcagcgccag
     8341 gagccttaac cagaaccatt gtgtcgccca gcggctgtcc aaaggtgatg ccatcagcat
     8401 gagcaataat cccaccactc attccgtaat aaatctggct gctgtcacca ttccgactgt
     8461 aaccgacatt cgtattaccg taagctccac gataattaag agaactgtaa ccactggtgc
     8521 cagacgatgt attacctccg tgggtgttac cgacctgaac gctataattc aggttattat
     8581 ccggcagcag agtgccataa acccccgata gattggtcat gccgcctttc aaatcgtttg
     8641 acatactgta actggcgttt gaattacgaa atgccgactg actgtctgta cgcatccaat
     8701 gactgaaggg aacattaagc gtaaaagcga gtaaatgatc ccgatcgttt tgccatatat
     8761 tattggaata gctgtaattc agcgaagtcg taatatcacc aaacggcaca tttaatccaa
     8821 atgatatttg ctggtcgctg cgtgacgtgt tccagtaact ttggcgactg gcactgaaaa
     8881 atgtcgtacc gtaatttcca agctgctgag agatgcttat ttgttcctga ccacgcttac
     8941 tgtagaacag attaaaataa tcaataaatt gtgtctgctc attggtgtct ccggtaggag
     9001 gcttgacggt gtaaccactc attcgactgt aggcactgtc ggataagtta taaaaacctt
     9061 gcgtagaata gcgatatcct gcgacctgga tattggttcc tgtctggtag aaggatttgc
     9121 tgtagacgga tttaaccgat tgcccgctgt gacgggtgcc atctgcaagt gtcgtattgg
     9181 cctgcgtgat atcgaaagaa aaggcaccaa aaagtcctaa atctttacca ataccaaggt
     9241 tgaaggcctg ataatcttct gcaatttgca ttccgccata aggtgtccag tttccttcca
     9301 gtccatgcat caagctacct tgtatgaact tgggggagct ttgcaggtta tttccactac
     9361 gatattcccc catggcaagc gcataacgcg tatatccagc acgttggaga accggaacag
     9421 acgaataagg aacatataat gtctgaatac tgccgtctgc ctcttttatg gttacttgca
     9481 gatcaccacc attggcggca gagttgatat catcaatagt aaaagggccg ggtggaacag
     9541 tagtctgata aacatcgtat ccgttctgtt ttacactcac ttgtgcggtg ccacgggcaa
     9601 taccatgaat caccggagcg aaaccatgtt ggctatcgag caacatcgct tctgttgaat
     9661 ttattttgag gccacgaaag ttcacactat caaaaatatc gccatcggta taactatcac
     9721 ctaccgttaa ccgtgagcgt aagggaataa tgtcacgttc agccgacgta ttgatatgct
     9781 gccatttatt gctgttagat gaattgctac tcccactgtt ataactccac gttgagttat
     9841 cgcgcagtcg ccaactacca atgttgatgc cactctgtaa attcaaatat gcatagttgg
     9901 attttcctgc attatggtta ctacgattat taatggaatt accattaaaa ctataattta
     9961 gaagcccagc atttattcct tcttcccaca gttcaggaga aacatagccg cgagccattc
    10021 tgccaacata aatttgcgga acggagagtg acaaatgttg ctgaccaaca tcaaactcag
    10081 ttgatgcgtc ggccaaacga tcctgaagtg gtgtgcatgc gctgttgtcc ggcacatgtt
    10141 tttctgcaga atgttcctta ttgtctaata acgcagattt ttttatgcca agactgacaa
    10201 gaaggtctgt actcagacat gggatgagat ctgcgttatt atcatctgca ataaaagtaa
    10261 tatcccgact agtcataaat tcgtcgttca aataaatgtc gacccgatat gtaccaggtg
    10321 gtgcctcgcg tcccttagta aaggcggata aatcgacaga ttcagcaaga tcattgctta
    10381 aaaaccgtgg attaaaataa tactctgcat caacgggaaa aaccgcagtt gctaagccaa
    10441 gggcaatggt aagccgtgca aagcgtggta gtattaacac ctgatgcata cgatatgcct
    10501 taaatgcggc acgaaaggct gtcattatta attccgattg agtttattat taattaagtc
    10561 actggcttac tgcatggttg ccttaatacg cggagttaat gcaccgtaat cattgatcgt
    10621 ctgaaaactg atatccccgg ttgcagcatg cgggatatcc accgatactt caccttttgg
    10681 cagcaccatg gtgtttggga gattgctatt tcctgctttc atatttgtta cggtaatgaa
    10741 gtagggcgtt ggattaataa gagtgagttt gctaccgctg cgacgaaacc gaagcattgc
    10801 tggcgcttct tcaggtgcca ttgccaggtt cgtggggcga taaaacattt ttatgcggct
    10861 aataattgcc agctgtaatg tattctcatt cttctggtct ttttccatag caggaatagc
    10921 tttaacattc acccagaaca ggctttcacg gtcgcctggt aactgatgat tagtagcgtt
    10981 gattatccgc agcgtattct cttttttacc ttgcatggaa aaaagcggtg gggtaatgac
    11041 aaactgggtg tctttttggt cacctgcatt ttctatccat gactgaatga gatagacact
    11101 ggcaggatca ttattcgtga caggcaataa aacctgtttc tggtttgccg gataaattac
    11161 ccgcgtcgca cccagtgcga cagctgcgtt ggcaacggtg ctatggcaag caaagagcaa
    11221 taaaagtaac actgtcgcca taaaggaaat agagtagggg gtacgcgtag tttgcatctg
    11281 atgcattctc catgtgttaa gaggataagc gggaaaaccg ttacatcaga caatcgatta
    11341 atgttgctaa tacattggcg agtcgccggg tttccagact taatgcgttt gtaataaatg
    11401 ttggttctct tttaatagga aaaccttgct acaaagtaaa tattttaaat aacagtaagt
    11461 ttaactactg ttgtttattg atagtttaga tattatgtga tttttcatag cgctccaaat
    11521 acaaaccatt tctcattaaa aatgagatga atattggtaa attgctcggg tgtgcgtagc
    11581 catgccacac acaccctgtt tcaatttcag atgacgtata gccaccttaa gattgaatta
    11641 tagatattga actttgaagg tcgcatcagc gtttgcagta ccagctacgg actgcccgag
    11701 agcaatataa cgagcctgga atggcagtat attcgtgcca tcaatcaagt cggttttagc
    11761 actaaaagtg gccccatcaa gtattaatac ctcacccgta cggtccaaaa tttgaatacc
    11821 gacattttgg gcagagcctg ccgctgaact ctgcagcgct aacgtatcgt cattactggt
    11881 gacagtagta cccaggaatg caacagctgc attactggaa acattggtat cacaatcatt
    11941 cagcttgata ttgaagccaa cggcggagct gaggtcaccc gctttagcca ggcgagaaga
    12001 acgaacctga cccagctcaa ccgtttggtt cattgattct gaatcaattg cacaagcggc
    12061 atttaccact tcacctttaa aatgaataac accgccatta acagtggtcg ttactgattc
    12121 atcaccctcg gctgcgctta ccgcagcaga cgacatcgcc aacacagaaa cgacaatcat
    12181 accaacatgt ttgagtttca taattctcca aaatttatat ttaagtagct gaataaagag
    12241 ggttaacaat taagtcattc tgttattgct taaaaacaag aatcattctt gattggttgt
    12301 tgccctgaga atatagtttc cagggttatt acatttcacg aagaacgata atatctttaa
    12361 atcacaagga cacctaatca caacaagttt attgtgcact tgttgaagag agtatttgca
    12421 aaaacatttt atgtcgctaa ataacatgac gatttcatta tgcatgttat ttataaaccg
    12481 tgactaaaca gaatattatt gcgaacaagg gaagtgtcag gaaaggtcaa gggctaccaa
    12541 tggttaatat ggctataaat cattagctat ggttaatgtt tgcttgctga aactaacacc
    12601 gaattgctct aaacagataa gtgcagggtt tattaaagct gaccgacaga ctttcttaac
    12661 ctggtaatat ctctcttggg tgactctcca aacatccgcg aatattcccg actgaaatgg
    12721 gatagctttc gtaaccgaca gcataggctg ctgtggtgac atcgtaatgt tcattcagca
    12781 tcggtcgcct gacttcattg agacgtagcc atttctgata ccggagtgga ctcattcccg
    12841 cttgatccgt ccttaaataa gtaacagcac cctctggtgt tttcgtatgc agtgtctatg
    12901 cgacagacac tgcgctctta gcatggggca gggaataacc aggctatttc tccatgcaca
    12961 ggccaattat gaaagaggag aggcttaagt tcttgaaccc tgaacgaata acgactacca
    13021 gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg cgcactgtta
    13081 tagcagttat cgataagaca aaaagatgcc ccgttaaaag cagtgcgtac aagagcgtta
    13141 tgtgtaaatc gggcgcggcg atcgccattt tcgccgcacc cgattcaaca taacggcata
    13201 taccttatac gcactgcaat gagatgcggt aaaaacagac tgaaagcgac accgaccctc
    13261 accagaacgc tgtcaatcat caattccatc aacatgccac atcaatgcat ggacgactca
    13321 cgccttgcgt cgttcgtctc gctgactgcc cgtttaaagg cggtctggca ggcagcgcgt
    13381 tgcgctgtct cctgctgctc tgtcatctct cactggcttc ggcgggctga cagctgcgtc
    13441 acatgaaaca tcatcatgcc gccttccatg ccgctggcgc ggcatcactt gccaaccctg
    13501 cgagcctgaa gcaccacaat aatatccgtc cggttatcct cgtctgattt gcctgtaaaa
    13561 cccggcaaaa atgaaaaacc tgtccgcgat tgcgtcagct tactgctggc aagaccacca
    13621 agaacaagta attcaccgtc cttaacatca acctttgtgc tgatttcccg cttcgtcagc
    13681 gtcggactgg agttaacacc cgtcgttgtc ttcacaaaat cactcagttg ctggctgata
    13741 tccagcgtta tcagtccgtc aagcacaacc ggcgtcacgg taaaaatcgc cccgctgttt
    13801 ctgtacgtca cagactgaac aggcgtaccg ctgctgccct gatagctgac gctgtccagc
    13861 accggaactt cctgacccac agtaaactgc gacttactgc cggaaagcac cgtcaggcgc
    13921 ggattactca cgacattaaa gcgtgaatcc tctttaatca gactgtaaaa cgcattcagc
    13981 cccccaacgt tcagcgtaaa cgaattaccc ccatcaagac gtgcacccac agacatcccc
    14041 agtttatttc ggaacaaatc agcaataatc tgaagccccg ttgcattacg gtctgtcgtc
    14101 tggacttcaa gaacatagcc gctcaccgtt acctgctcag ccggaacgtc gatacgcggc
    14161 agtatttttt ctatctgccg gacggacgca gccggaccgg aataaaccag cacctcggca
    14221 tccgtcgcca tcgacatacg cgtcgatgcg ctgtctgtcg tggatgtgcc ggacacaccg
    14281 ccagataaat aatcaacgga ctggatatga ttactgaaag cccccgaaga aaccacagag
    14341 ttaagcatgg ccgacaaata actcacgctg cggtatcgtg gacgataagt aaaaaccaca
    14401 tccggcactt tctgctcagt ctgctttatc actgaaatgt aatccacgcc atccttcatc
    14461 gaggtgacgc tgatattgag gcgtttaagg tatgaaataa aaaagcttcg cggctcctga
    14521 tttttactca gatagaagct gacaagcctt gtgtctcctg caatatccgg cgacaactga
    14581 taagggcgat taaaaacccg ctgccagacc atgctgataa cctgcggtaa tggcgcattg
    14641 tccattgaca tctcaacacg ccctgcctca ttttctgaaa cggcataagc cggagaaaag
    14701 aatgataaaa gcacgaatga aaagaatact gttaccagct tcatttttgc cccctgacag
    14761 aaaccggatt attacaggac catgcaacaa ctttcacgcc atccacttcg ccctcaagca
    14821 taatattcag ccccctgaat ttatttgcgg aaaccatacg aaggcgattc tctgtatcgc
    14881 gcagaaaaac ataattccgg tccccgtcat taaaacgccc tattacgcac cacttttgag
    14941 aaggcgcaga agcggtttta ttctccggta aagacgaaac attaaccgac ggcgaagacg
    15001 gcgaggaacg taacggcgct gactgctgtg cctgttctgt ttttttctgc ggattaaaaa
    15061 aaggcaaaag aaaactaaag aagagataaa cagaaagcaa tataagaaaa acaaacaaaa
    15121 caataacctg cggtttaaac aaaactgccc tggcatcagt tcttgtttcc accgcgccct
    15181 taacatcatg agagctgtac agagggaaaa tatctttgct gtaacggcag acataatttg
    15241 aaagatgcgt tgcctttgtt gttctggaac cgtcaaaaat atcaacacga taactcttat
    15301 gagagagacc aatcagtttt ttcattctga acgtctgctc aatacgttca cgaagaaaac
    15361 ggggcagcgt tgtcagtgcc tgattaataa gaacaaggtc gcaggtctgc cctgtttttt
    15421 catcagcata atgacgatgc tcagcagcaa aaatgcgggc atcagatgac aatgctttat
    15481 ctgatgtaaa aaaacgatgg cactcatcaa gaataattaa atcaccgggc tgacaaagtg
    15541 atttatcctg atttcccttg accggataga aatcaggtgc catcacccgt tcattttcaa
    15601 catgaatgat ttcgcctggt gacacatcat cagatatcag tttattttta tgacaatact
    15661 gaacaatagc ctcataattc agtccgtaaa tattagagac aatcctgcgt ccggatgcaa
    15721 cagccggaat aataacagac ttaacaacct cataagattt gccggaaccc ggcacaccaa
    15781 tatatgcagt aatggccata aatcacccgt ttaattaaag aatggaatgc ggcgtataaa
    15841 gaaacgcgtg aacatggctg aaacaatcat gggcaaaaca acatcaaaag acatcagatt
    15901 gagaaaccac aaaacagaag aaggcaactc tgaaaaaagc gatgaaatac tgctgccatc
    15961 aggaagatga gtcacaatca acggaagaac ctcagcaacc ataatataaa ttgcagagaa
    16021 cattacaaac ttaatgagtg cagtacgaaa aataaaacca agagcagtat ttattgcagc
    16081 aataaaaata gcaaacataa ttcaccacct gatatcagga acgaagaaca ataaagaatg
    16141 aaagcatgga gccaatcagc gtcataatca ccctgaaagt ggccctgact tcatcggtat
    16201 caagcagcac acaaaaagta tccagttgat aatctttatt ccagatattg aatgtaaaaa
    16261 caggacactg accccgtgga attgtcagtg aaaaatcacg cgcagaaggc cacatatccc
    16321 acaacggaga gataaatgat gatggctcag gtgatgaaat atctggcgtg gatacatccg
    16381 gtgcagtacc aaaatcattt gtcacggtca cattaacatt taccgtggca tcacctgtta
    16441 ccgtggtact ggcagaccct gttccgacgc cggttatcgt tccggtacct gttccggtac
    16501 ctgttccggt gcctgttccg gtacctgttc cggtacctgt tccggtgcct gttccggtgc
    16561 ctgttccggt acctgttccg gtgcctgttc cggtacctgt tccggtgcct gttccggtgc
    16621 ctgttccggt gcctgttccg gtgcctgttc cggtgcctgt gccgctacct gttccggtgc
    16681 ctgttccggt gcctgacccc gttccgccac tggagccaga actgccatta ccagaaactg
    16741 gggtaaaatc cggatcaaca accggaggcg tatatttacc gccagatgat tccgtgtcac
    16801 cttccggcgt aggcggtatg gctatttctc ctgttccggt gaaaccaccg gaatcaccac
    16861 cggacatatc tacagtcgag cgataattat caacatcaga agcagtaaaa tacaatgaag
    16921 acgatgaaac cccgtcataa gatgaaaggc catctaactg ctttgtcagt ccattaagaa
    16981 tatcggcaat atcctgagaa gcaagattat taccggaaac agaaggtgtg gaatattcag
    17041 atgaaaacgg attttcagta accgctgcaa tacaggatga gctaccccct gaaggtgtaa
    17101 aaagcacctg tccctggtca caataagtat cagaatcaga acctaatact gaagaataca
    17161 catataaaga actttcagac catcgaccat tatacttaga aatatagtaa aaagtataac
    17221 cttcaagaga accaatggtc tttttagggg tgtaataaga acgagtcaga gacggagacg
    17281 aatctgccgg aagacaattt aaaagctgcg aagccgttga aaaaggcgat gtactggtag
    17341 caacgccaca ggaagacaat cccccaatcc agactttcga cccactggac atggcaggtg
    17401 tcgagaaaaa ggcattatac ccgtttttct caagagccag ctttgcaatt tcttccgcaa
    17461 aaggtttacc aacagcataa ataccaatac tgacagtcgt tacacccagc ccataagcaa
    17521 gcgtactcca ggtcattgcc gaagatgata ttccaattga agccgcataa tttgacgcaa
    17581 aagaagctgc cgcacgcgaa taaccggaaa gcgttgcaga tattgcggct gcatcagcac
    17641 caagagaaat cagtttacgg ttaatcacac ctgataactc agatgtaaaa gtatttgttg
    17701 ccagtgaagc atcagcacgg acattacagg agaaagacag agaaaataat aaaaacgctg
    17761 tcattgtttt atttaatttc acaaccaccc ccaaaatcaa aagagaaagg cttaacaatg
    17821 ttgcgaaaat cacggtcagc aacttctgaa acagatgaaa caatataatc atcaacagtt
    17881 atattctgtc gttcgataat tttttctatc ttctttatta catcaacggg tatttgtagc
    17941 cggatttctg tcttttgcat aaaaactcac atattactct taaaacccat aacacaacaa
    18001 agcccacaaa gagagccgta catcatgaaa ataaattccc acataaagaa ctccgatcaa
    18061 aaaagggcgg atatccgccc ttccccataa aaacaactca gatacttctg atggctcccc
    18121 atactttacg gacgcctgca aaagtaagat aaactgtaac tcctgcaaca ccgatagaaa
    18181 gaataacggc agaaacagaa gacatatcaa tactgctagt cagcgatgaa aaatcgactg
    18241 tactttcagc aaaagacgga gatgcagcaa gagcagaagc agcggaaaaa gataaagctg
    18301 caactttctc attagcaccc ttaaaaacat cagtcttttt tgcaaacata attaaagtct
    18361 ccttattaaa ctccagattg tggaaataat catgccaaga aaaaaacaaa atactggcag
    18421 tgaaaatccc gttaaaaacg ccgaagaatc aatccccgac acatcaggta ttcccacgca
    18481 aacagcatta atttgcgtgg aaaccgaatc cgaattagct gacataaaac ttaactcttg
    18541 tctttacctg cgggcagtgc ccc
//
LOCUS       DAHBHC010000098        18349 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHBHC010000098 DAHBHC010000000
VERSION     DAHBHC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.099"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..559)
                     /locus_tag="M2A35_004180"
     CDS             complement(<1..559)
                     /locus_tag="M2A35_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCB7200403.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTE"
     gene            complement(643..1806)
                     /locus_tag="M2A35_004181"
     CDS             complement(643..1806)
                     /locus_tag="M2A35_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCB7200404.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(1809..2447)
                     /locus_tag="M2A35_004182"
     CDS             complement(1809..2447)
                     /locus_tag="M2A35_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCB7200405.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(2457..2855)
                     /locus_tag="M2A35_004183"
     CDS             complement(2457..2855)
                     /locus_tag="M2A35_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCB7200406.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKIIPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(2873..3532)
                     /locus_tag="M2A35_004184"
     CDS             complement(2873..3532)
                     /locus_tag="M2A35_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCB7200407.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(3583..4281)
                     /locus_tag="M2A35_004185"
     CDS             complement(3583..4281)
                     /locus_tag="M2A35_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCB7200408.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(4300..4701)
                     /locus_tag="M2A35_004186"
     CDS             complement(4300..4701)
                     /locus_tag="M2A35_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCB7200409.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(4828..5559)
                     /gene="rlmB"
                     /locus_tag="M2A35_004187"
     CDS             complement(4828..5559)
                     /gene="rlmB"
                     /locus_tag="M2A35_004187"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCB7200410.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(5739..8180)
                     /gene="rnr"
                     /locus_tag="M2A35_004188"
     CDS             complement(5739..8180)
                     /gene="rnr"
                     /locus_tag="M2A35_004188"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCB7200411.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(8219..8644)
                     /gene="nsrR"
                     /locus_tag="M2A35_004189"
     CDS             complement(8219..8644)
                     /gene="nsrR"
                     /locus_tag="M2A35_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCB7200412.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(8849..10147)
                     /gene="purA"
                     /locus_tag="M2A35_004190"
     CDS             complement(8849..10147)
                     /gene="purA"
                     /locus_tag="M2A35_004190"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCB7200413.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(10251..10448)
                     /locus_tag="M2A35_004191"
     CDS             complement(10251..10448)
                     /locus_tag="M2A35_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCB7200414.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(10530..11534)
                     /gene="hflC"
                     /locus_tag="M2A35_004192"
     CDS             complement(10530..11534)
                     /gene="hflC"
                     /locus_tag="M2A35_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCB7200415.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(11537..12796)
                     /gene="hflK"
                     /locus_tag="M2A35_004193"
     CDS             complement(11537..12796)
                     /gene="hflK"
                     /locus_tag="M2A35_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCB7200416.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(12882..14162)
                     /gene="hflX"
                     /locus_tag="M2A35_004194"
     CDS             complement(12882..14162)
                     /gene="hflX"
                     /locus_tag="M2A35_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCB7200417.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(14239..14547)
                     /gene="hfq"
                     /locus_tag="M2A35_004195"
     CDS             complement(14239..14547)
                     /gene="hfq"
                     /locus_tag="M2A35_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCB7200418.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(14633..15583)
                     /gene="miaA"
                     /locus_tag="M2A35_004196"
     CDS             complement(14633..15583)
                     /gene="miaA"
                     /locus_tag="M2A35_004196"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCB7200419.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(15576..17423)
                     /gene="mutL"
                     /locus_tag="M2A35_004197"
     CDS             complement(15576..17423)
                     /gene="mutL"
                     /locus_tag="M2A35_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCB7200420.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(17433..>18349)
                     /gene="amiB"
                     /locus_tag="M2A35_004198"
     CDS             complement(17433..>18349)
                     /gene="amiB"
                     /locus_tag="M2A35_004198"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCB7200421.1"
                     /translation="RVETPAVVAPRVSEPARNPFKTESNRTTGVISSNTVTRPAARAT
                     ANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNDDPMFKGVLTRD
                     GDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQ
                     HEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMISQLQRIGEIH
                     KRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNYFL
                     AHPMQSAPQGATAQTASTVTTPDRTLPN"
BASE COUNT         4249 a         5146 c         4467 g         4487 t
ORIGIN      
        1 tttccgtcat tcccatgcca atcaacaaac cgcgtttttg cccacctggc aataagtgag
       61 aatcgtagcg atcgctcagc agcggcgtgg tccagtcctg aaacggcgcg ggtaacatct
      121 gtaacagcaa tggcgtggcg gcaaaggtca tggttatcgg acataacgac cctgcctcaa
      181 cctgtgcgtg caacataaaa cgcgccgcgc gcgccacaaa tgcgccggag cgagcgtctt
      241 cttcccaggc aagattgtgc acccgattgg tacatagcgc ctgcatcagc aggtgccagg
      301 cggggtgaaa acgcacatcg tccagacggc gtccttgcgc atcgtagcgc aataattcag
      361 gcggattcac attcgccagc cgccccagtt caagggattc agccgttcct aactgctgac
      421 caatactggc tagaaaatcg ctatcccagc cagcaccttc acgcgttacc gcttcgcaga
      481 gcgcgccatc agacaggtat aagttgctgt tatttaatgg tataggttga ttaaaaacgg
      541 tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg cagtcaccat tagtatggtc
      601 actgccatgg attcatgaca gaaagagatt gctaaaacat tcttatccag caatgtaatg
      661 cggaacgaaa cgtgaggtgt ctttggtgat cagtgtgtta tcttcacgga tccccattcc
      721 gcacgcttca tcatcgacaa tccagctacc gatgagtgtg tagctatcgc caaatcgcgg
      781 cagaggttga aacgcctggt agatcatcgg ttcatcggcg taatcaccat cagcgtggtc
      841 aacgacgtta ttctgaccgt cgaaaatggt gacgtttccg ccttcgcgcg agtagatcgg
      901 tttacgcaca tagctttcgc cagcggcaat ctgcgatttt tcaccctcaa accaggatgc
      961 aagaaggtta ggatgaccag ggaagaagcg ccaaagcagc ggcattagcc ctttattact
     1021 caagatactt ttccataacg gctccaccca ttgctcgcga cgcttgcaca gcagcggacc
     1081 gttatcgtca cgcatcatcc actccagcgg atacagctta aatgcacgct ggatgacatt
     1141 atcatcaaga tcggtcagta caccgccgac gcccaaaccg agatcttcaa tgtagataaa
     1201 ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg tagtcctgtc
     1261 ttcatcggtg tcctgacagc agcagaaata aaacggctcc cggctataaa gctcgctaaa
     1321 gcgtgaaatc aggcgttcct gaatggcatt gtactgatcg gcatcacgcg gaataatgcc
     1381 gctgcgccgg gcatcctcca gccacaacca ctggaaataa gccgactcat acaacgaggt
     1441 cggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc aggcaaaatc
     1501 catccggcca tacagcgaag gatcgcgtgc gcgccagctt tcggcgatca catcccagta
     1561 caacggcggg atcgccagtt gcgtcaggat ctcttcgtct ttaaccgcgc gatccaccac
     1621 ctcaaggcac atctgatgca gttccgcagt cggtttttcg atctgctctt caatctggcg
     1681 cagagtaaaa cggtaagccc gactctcgtc ccaatagatt tcattgtcga tgatatgaaa
     1741 gtcgaaaccg ttgttagcgg cgatctggtc cagatcccgt cgcacaggaa cgttgtgtct
     1801 cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata gccgccgcgc
     1861 gaaacggttg acgctttttt ggtggtaaag ccgcctgaag agtaagactc ttttttgcca
     1921 gaaccggagc gccaggagta atcaccggaa gtgttgcgcc agacggggcg ggaagcaaat
     1981 gaggatccgc cgctgttata gacgaacggc tcatcgcgat ctttacgaat aactcggctt
     2041 aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cattgtcgta gtagcaattt
     2101 tcgtacttag actgacaatt ctgctgagtc atattcttcg gaacatcggc ataaaatgcc
     2161 gctttggcgt tgttccagcc acgcgcgcaa atgtcggcat tattaccgtc atcaatacaa
     2221 tcctgcactg tcgaataaaa cgttccgtcg ccgtcgttat cgacatcgcc actttcactc
     2281 catcctttga ctattaaaaa aacggcaccg cccattagca ccaacgttaa gtatttttgt
     2341 gcgtagcgat tgcggcgcgg ctcaaaagga ttatgcggtc ttccaatgcg actgatcgct
     2401 ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc tggccatgat cgtccattac
     2461 catgtcatac aagctgcgtt aaaaatacca ccagccagtg cggcggttcc cataaacatt
     2521 cctgctgcgg tattgtgatt aataattttt tcgcttaatg cgggcatata aagtctgaca
     2581 ccagcaaaaa cgagtaactg aatcaccagt gcaatcccgc cccaggcgaa atagtctgga
     2641 atactcaccg cattgattgc cgcactggat aaggggatca cgtaacccaa caatgtaccg
     2701 ctgaatgcca gtgacgccgc ggtattattg tttttgatta attgccattc gttgtgcggt
     2761 ataattttgg agtagataaa cagaaaaata atcaccatag ccacgccaat aaaaaaatag
     2821 gcactaaagg caagaagtga atccagtata tgcattgtaa acgtccttgt aattaaccaa
     2881 taatatgaag tgatgtcagc gggatatcga cacccaacgc gcgcgaaaat aaccactctg
     2941 gctcgccgag atcgttaaaa gattcctcac catttaacag caaatattcg taagtatctt
     3001 cggttacttg gcgttggtag cccatggtaa aattatggac ctcccatttg gcatggtttt
     3061 gattttctac tttttccagc atgtagacgg gttcgatatt tcccggttct tcgctgttaa
     3121 aaaatcgctg ccagcgtttt tcctgccagt ttaagatcat tgcccccatc actttggcgt
     3181 tgatcgcctc gcgccagtga ctctctttac tgataccgta gctctcttca tagacaaaga
     3241 gcttgatatc atcaatgtca tcaatatctt cgccgccggt ggtattgatt tgcagaaatt
     3301 catcgcctga agtgtagtag cggaaaatct gactaccgcc gcccagatcg atgtggctga
     3361 cagcggctac cgtaaattcc tcacccggca gcgcaatcag caattcatct tccagcaaac
     3421 gaaacgctaa cgtgtcgagc gtaaagccac tattgagatg aagtcccagc ggaccgcgag
     3481 cgatagcagg cttattatcc ttgccaaaca gacgctggaa aaaaccagac atattttatc
     3541 cttaaaatag ttgccagcct tttagcgagg ctggcaaaaa aattactcgc cctgttggcg
     3601 ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccaa ttccggcttc
     3661 cgccagcttt tcatcaagat cgcgaccgtc tgcgactttt tccaactgtg cagcagcatc
     3721 caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt ctgccgctgt
     3781 cgaaacgctg gaagatgcgc caacggtaga ggttgttaca gcctgttgtg cacgctgcat
     3841 ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac gctgcgctgt
     3901 cgctgtaact gctttttcca cgccatcacg ggagacttcc agattcgaca acacttgctc
     3961 ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt taatcaacga
     4021 cggattaacg ttcttgctta acgcttccag cgcacgggct tccagactgg ctttgcgctc
     4081 acgtaaatct ttcagcttat cgtgacttaa tttcacccgc gccagcagat caacgcgaga
     4141 ttttccggct ttatcgagtt cagctttagc gtcacgaata tgctgttcca gcatgcgcac
     4201 gccctgggtt tcttcaatgg attcttccgc ctgggagata aacgatttcc ccagcgtaaa
     4261 taaacttttt aaaattccca taaatacccg tccttgtaat tagtgtgaat attcttctgt
     4321 aatttcagcc agatccaatg cgttatctac cagcgaggtt atctcaagca ggatatcttc
     4381 aagagaagat ttaagcgata acgccccgaa aacaatgtaa tactcttcct gttgcacgct
     4441 ggagattcct accgatgaca acggcatcat cttctgattt cttaataaga aggtattaaa
     4501 ttcatctgga ttgctgatac tacttaccgg acaaataaag gtttcgataa tcatttggcg
     4561 ggaagtaaaa agaatattga tttgcaaatc gccataatca ttcattttaa taattaatgc
     4621 gctctcgcta tttgtcacat caatattttg ttcaggtaca gtttgcagcg ccgtcgccag
     4681 cgccaacgga ttccatgtca tatattattc catatagatt tagtttaaat attaataaaa
     4741 tgaatatttg cagtacgtaa ttatcttacc agctatagac aaaaaaaaac catccaaatc
     4801 tggatggctt ttcataattc agacgaacta gctgcgctga cgaaccgctt caaataagca
     4861 aattccggtt gcaaccgaaa cgttcaggga agaaacactt cctgccattg ggatgctgat
     4921 caactcatcg caatgttcac gggtcaggcg acgcatacct tcaccttccg cgcccatcac
     4981 cagcgccagg cgtccggtca ttttgctctg atagagtgta tgatccgcct cgcctgccgt
     5041 accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcaa ggttagtcac
     5101 ccgaatcagt ggaacgcttt ctgccgcgcc gcaggctact tttttcgccg tagcgttgag
     5161 ctgtgcggag cgatctttcg gcacaatcac cgcgtgaacg ccagcagcgt ccgcgctacg
     5221 caggcacgcg ccgaggttgt gcggatcggt tacaccgtcg aggatcagca ggaacggttg
     5281 atcgagcgaa gcgatcagat ccggcagatc gttttcctga tactgacgtc ctggcttcac
     5341 gcgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat attggcggtt
     5401 tgccaactgg ataaccacgc cctgagactc aagggcgtga atcagcggta acagacgttt
     5461 atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc gctccagcag
     5521 ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg ttgtttacgt
     5581 tttgcctgat gcactacgtt tatccggcct acatgatctc tgcaatatat tgaatttgcg
     5641 tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcattttg
     5701 tcagcaatct aaccctcttc ttttaaagag ggtattgatc actctgccac ttttttcttc
     5761 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga tggctttttc
     5821 gcttttctcg cgtctttctt cgccgctttc ggcttcgttt ttttctcacc gcggaaggcg
     5881 ctgtctggct caaagtttac ctttttaccg acctgacgac gcttgccgcc ttttttgcct
     5941 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttca
     6001 ctggagatca gactaaagtc gattttgcgc tcgtccatat taaccgcttc gacgcgaact
     6061 tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cccccatcag gcgttgccct
     6121 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
     6181 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
     6241 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc gacatcgcgc
     6301 gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg caacatctct
     6361 tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc ctgctctttc
     6421 gacagcagat atttaatggc gcggtgcagc gtcaggtcag gataacgacg aatcggcgaa
     6481 gtaaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc tggatcgtaa
     6541 atcgcctgtt tcatcgagcg tagcagcatg gtttgcagca tttctgcatc aggacggtcg
     6601 gcaaccgact ccagcagttc cgcgtagtca cgcggttccg gcttgttacc acccggcagc
     6661 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
     6721 tcgtgaatac ggaacagtgc cggttctttc gctttctcaa cgaaacgcgc cgccgagata
     6781 ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt cgttacgctg ggtctgttcg
     6841 atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
     6901 atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg caactcttcg
     6961 agatgcttaa ccagcggggc gtactgctcg cgcagatcct gatcgccctg cagaatatgc
     7021 cagactttgg tgtaggtcag acgcgcgtga gagctcatca ccgcttcgta gaatttgtag
     7081 cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
     7141 tgcgggttga gcgaacacag gccgttagag agcacttccg gcagcatcgg gataacctgc
     7201 gaagggaagt acaccgacgt gccacggtta cgcgcttctc tgtccagcgg cgttggcgga
     7261 cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc gccgcgtttt
     7321 ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
     7381 ggtaaatcgc gcaaatcgac acggcccgct tttgcttctt ccggcacttc ttctttcagc
     7441 ccggcaacct gttgctcaac agcctgcggc cagatgtacg gaatttcatg ggtacgcaga
     7501 gcgatatcaa ccgccatgcc ggtgcccata ttatcgccaa ggacttcgac gattttaccc
     7561 accgctttgg tgcggcgagt cggacgctga gtcagttcga ctacgaccac aaagcccatc
     7621 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg gctatcgtca
     7681 ggaaccacaa agccgacgcc cgcttcggta aagtagcgac caacaatctg gctggttttt
     7741 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc gcccagcggc
     7801 tgagccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga gagatacaaa
     7861 tcatctttac gcccttcaac ccgcagaaag ccgtagccat cacggtggcc aataacggta
     7921 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt gaagaccagt
     7981 tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg ctcttcgcct
     8041 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
     8101 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
     8161 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
     8221 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
     8281 agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt ttcaaccgac
     8341 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
     8401 cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccaggcga atgccgccat
     8461 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
     8521 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
     8581 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
     8641 gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg ataaaccagc
     8701 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag gatttaacac
     8761 tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt tagcccggca
     8821 ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cggatcgcgc agaatcatgg
     8881 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccagtc agctcttcaa
     8941 tacgcttgat gtagttcagc gccgcctgcg gcagaccgct acgatctttc acgccgaagg
     9001 tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc cagtcgtcag
     9061 ctgccagcgg agtggtcgtc acttcacgac catccggcat acggtaagcc acacagagtt
     9121 tcacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca gacagggagt
     9181 tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgcc
     9241 ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
     9301 gttcggtcgg gaacggacct gcacctacac gagttgagta agctttgagg atacccagaa
     9361 cataatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
     9421 tggaagaagt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta ccctgcgcac
     9481 cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
     9541 ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
     9601 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
     9661 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
     9721 ctactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
     9781 cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc agcggacatg
     9841 cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
     9901 ctttcatcag cgcggccgga gacagcacaa caccgttacc gatgatgctg gttacattct
     9961 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
    10021 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
    10081 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
    10141 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
    10201 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
    10261 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
    10321 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
    10381 taaagcatcg gccctaaacc ttccagtacc aaaaccagag caagcgccag ccagattgtc
    10441 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
    10501 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
    10561 tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc agagaagctg
    10621 ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt actgaatgca
    10681 tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac gcatgatgcg gccctgacgc
    10741 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag tttttccgct
    10801 tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc gcgcatacgg
    10861 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttaattcg cacatcgaca
    10921 acctcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt gcccttcgtc
    10981 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgccgccgg ggtagtaact
    11041 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
    11101 ccacgggaat cggtgacgat atctttcacg tccaggcgac caatttcaga acgcagacgg
    11161 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
    11221 aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac gatcaggtct
    11281 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
    11341 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc ataaaccaga
    11401 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgctcacct
    11461 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
    11521 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
    11581 ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct ccactggttg
    11641 tggaggaaga ggctggcggc agacgcagca gattgctggt accgttatcg ctcttcgccg
    11701 caggggcgtt accgcctttc agcatctggt ctaacggcag aaccatcagg ttgccacctt
    11761 tatcgttaac cagcactttg cgggtgttac ccaacacttt ttccatcgtc tcgatataca
    11821 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
    11881 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
    11941 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct tctgcttcac
    12001 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
    12061 cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc atgtcatacg
    12121 gacggatagt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
    12181 tcagaatgcg gtccatggtg tatttaccga taaccccgcg cagggcgctg tcggtagcct
    12241 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga ttggtgacgc
    12301 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
    12361 aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg ataaacgtcg
    12421 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
    12481 cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
    12541 ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
    12601 caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg ctcagtttgc
    12661 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
    12721 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
    12781 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
    12841 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
    12901 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
    12961 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
    13021 cttctcagac gcccttcctg cggcggcaaa cgcaatgtat gctgcgccac ctcgccggaa
    13081 agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg tgcggaaagc
    13141 cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa atcttccagc
    13201 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat ctcttcaaga
    13261 accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcatc aatgacgtgc
    13321 agcagtaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc caccagatcg
    13381 tgcggcaggt ggcgaataaa ccctacggta tctgcaagta cggtttcacc gacatctgca
    13441 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
    13501 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
    13561 gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
    13621 actctttcca ggcgtgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
    13681 tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct ttcaaggtgg
    13741 gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc aacctgcaac
    13801 ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
    13861 ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
    13921 tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc aactgcttta
    13981 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
    14041 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
    14101 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
    14161 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
    14221 aaaaaacagc ccgaaacctt attcggtttc ttcgctgtcc tgttgcgcgg aagtattctg
    14281 cgcgctgcta ccatgatggt agttactgct ggtaccgcca ccggcgttgt tactgtgatg
    14341 agaaaccggg cgagacggga caacagtaga aatcgcgtgc ttgtaaacca tctggctgac
    14401 cgtgtttttc aacaggatca cgaactgatc aaaagactcg atttgccctt gcagcttaat
    14461 accattcacc aaataaatag aaactggaac acgttcccga cgcagtgcgt tcaggaacgg
    14521 atcttgtaaa gattgcccct tagccattct ctcttttcct tatatgctta tttgtacttt
    14581 gaacctttcg attctgaaaa aattgcgcac gatacgtctc aattgtacac attcagcctg
    14641 cgatagcacc aacaacctgt aatacttcgt cacgcgcctg ttctggtttt tcactgtcaa
    14701 gccagtgaac cccttcccaa ccacgcagcc aggttatctg ccgcttcgcc aactgtctcg
    14761 tggcgcaaac acctcgataa accatttcat cgtatgagat ttcgccttca aggtaagacc
    14821 acatctggcg ataacccacg caacgaatgg aaggcaagtc cgtatgcaaa tctcctcggg
    14881 caaaaagcgc ccggacttct gcttcaaaac ctgaagccaa catctgatga aaacgctgct
    14941 caatgcgttg atggagcagt tcacggctcg ccggggcgat ggcgaactga tgcacctgat
    15001 acggtagagc gtctcctgac gtttgcgtca gttccgttaa agttttaccc gaaatgaaaa
    15061 aaacttccag tgcccgggaa agcctttgtg gatcatttgg atgaatcctt gctgccgcaa
    15121 ccggatctac ctcctgaagt tgacgatgca atgactccca accttgctct gccgcctgtt
    15181 gctcaattct ggcccgcact tccgggtctg ccgacggtag cggcgacaac ccttccagca
    15241 atgccttgaa atacaacatc gtaccgccca ctaacagtgg gatccgcccc gccgcggtga
    15301 tatcggccat ttccgccagc gcatcgcggc gaaaatcagc agccgagtaa gcctgcgacg
    15361 gatcgcgaat atccagcaat cggtgcggcg cggcgagtaa ctcttcagcg ttcggcttcg
    15421 ccgtcccgat atccatccct ttgtaaataa gggcagaatc aacgcttatc aactctactg
    15481 gtaaaatttt acgcagctca atggctaacg ccgttttacc ggaggccgtc ggccccatca
    15541 aaaaaatcgc cttaggcagg ctcgccttac tgatatcact catctttcag ggcttttatc
    15601 gccggatgta aatcaacaga ttgtaacaga ccacccggcg gcgttttcac aagttgcgga
    15661 cataaccgtt ccacgtccgc cagcagggtt atggcctgtg ccattgacca ctgcgcatgt
    15721 tcgctcatca gatttcgtgc aatccactgc gcaatattgc caggttcgaa tacggactgc
    15781 ttcgccaggt agcctatcag ttcaggaatc aagatttgta aattttgttg gcgtaagggt
    15841 aaaggcacgg ccctgatggt cacatgctgt gcatctgact ggaaatcaat acccaattcc
    15901 gctagggcag actgcgcttt ttctaatgcc gatttttctt cggcagaaac ttttagccgc
    15961 aacggaatca gcagcggctg ggcgcaaacg ggcgcttcac ccggcgtcaa ttgtgcctga
    16021 cgtagccaac gttctgccac tggcaaggat aaaagtgaaa tgttgccatc gcgctccagc
    16081 aacgcacagt cggaatggac gatagtcagt acccgaccaa aactctgact gttcgccgca
    16141 agtgcaggtt cctgcggttc cggcgctttt ggtttttgca tcggcgcggg cgtttgcaaa
    16201 agctggcgat acacttcacc ttgctgtttc tggtagcctg gctgcgcatt cggccagggg
    16261 gcagccggac gactgcctga tgctggcgca ggagtgtagc gcggagctac cggctcacga
    16321 gctgccggtt ctgcaaagtg attgcgcccc gccgccacgc ggttttccgg aatggaacgc
    16381 ggtgcaggtt ggggttcatc gtccagcggt agcggcgttt ccagttgctg ttgtagcacg
    16441 ctcagcacgc cctgatagat aaaatcatgc accagacgcg actgatggaa acgcacttcg
    16501 tgtttggcgg ggtgcacgtt gacgtccacc tgatgcggat cgatctccag atacagcaca
    16561 aatgccggtt gctgatcggc ccccagtttg tcttcgcagg cctggcggat cgcgtgattg
    16621 atcaggcgat cgcgcatcat gcgaccgttc acgtagcaat actgaatttc tgccagtgcg
    16681 ggcgtggtgt gatttggatc ggccacccag ccgcgtagcg tgagatcgcc gtgttgccat
    16741 tcaatcgcca gcgcttgttc aagaaaagcg gtgccgcaaa tcgcgcctaa gcgccgttct
    16801 ttttgcccgc cttccggcac tgcgcggtac tgacgcacaa ttttaccgtt atgcgacagg
    16861 ttgatcgtga cgtcgaaacg cgccagcgca atgcggcgga tgatctcatc aatgtggtta
    16921 aattcggttt tctcggtgcg caggaatttg cgccgcgccg gggtgttgta gaacagatcc
    16981 agcacctcca gcgtcgtccc cacaggatgc gccgccggtt ttaccgtcac gttcatatcg
    17041 cgcccttcgg cataggcctg ccaggcttcc tgctgttctg cggtgcgtga agtgagcgtc
    17101 aggcgggaaa ccgaactgat actcgccagc gcctcaccgc gaaagcccag gctgataatg
    17161 gcttcgagat cgtccagaga ggcgatttta ctggtggcat gacgcgccag cgccagcgcc
    17221 agctcgtctt ttttgatacc gcagccgtta tcacgaatgc ggataagttt cgccccaccg
    17281 cgttcgatat caatatcgat acgtgtcgca cctgcatcga ggctgttttc caccagttct
    17341 ttgactaccg acgcaggtcg ctcgaccacc tcacctgcgg caatctggtt cgccagttgt
    17401 ggcggtaaga cctgaattgg catcaatcgt ccttagtttg gcagcgtgcg atctggcgtc
    17461 gtcaccgtac tggccgtttg tgccgttgcc ccctgcggcg cagattgcat cggatgcgca
    17521 aggaaataat tgcgcaggcc tttgtaaatg gcttctgcca gctgttgttg gtaatcgtcg
    17581 ctcgccagca aacgttcttc gctgttgttg ctgataaaac cggtttcgac cagtactgat
    17641 gggatatccg gcgaacgcag aacgccgaga ctcgcgtgtt ctggtcggcg tttatgtatt
    17701 tcgccaatgc gttgcaactg actgatcata ctggtcgcta catcataccc tacccgttgg
    17761 gaatgaccga actgtaaatc cagcaccgcc tggcttaaat aggggtcaga ctgactgttc
    17821 gccagcacat cacccgcccc acccagcagc tccgactgtt tctcgtgctg ctccagccag
    17881 ctggccattt cgctgttggc gcgacggtta gagagcaccc atacggaagc gccagtcgca
    17941 ctgcggttcg gtgcggcatc agcgtgaatc gacacgagga aattggcgtt ttgcttacgt
    18001 gccacatcac tgcgccccat caccgagata aagtaatccc cgtcacgggt taaaacgccc
    18061 ttaaacatcg gatcgtcatt gagcaaagta cgcaatttac gcgcgatggc gatggtgaca
    18121 tttttctccc gcgtaccacc ggggccgata gcgccagggt cctgaccgcc gtgtccggca
    18181 tcaatagcga tgataatttt atcgccagtg ttagccgtcg cgcgcgctgc cggacgcgtt
    18241 accgtattac tgctgataac acccgtagtg cggttacttt ccgttttaaa cggattgcgc
    18301 gccggttcgc tgacgcgcgg tgcgacaacc gcaggcgttt caacgcgtt
//
LOCUS       DAHBHC010000099        18167 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHBHC010000099 DAHBHC010000000
VERSION     DAHBHC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.100"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(177..1496)
                     /locus_tag="M2A35_004199"
     CDS             complement(177..1496)
                     /locus_tag="M2A35_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HCB7200422.1"
                     /translation="MSDAAMIIAPTDIREMVREVETAYYRYLEEFRIPDDHKIVVNFS
                     AGKDSTTTATIAHHLFGDRVKNVMADTDNEHELTVDFARNVHHQIGCKPVQIVKRIYT
                     EAEFERRRQSLKDRWPKRQAIRMGAYRGVIMPSLARADTKFGQAWLRTAERWGIEFET
                     PLEAAISVMHPSGNSFLDAALLHGKFPMLRDRFCTDELKIQIAFDAAIKPLLDDGEVV
                     VQWSGVRADESDKRAGYSRFARDERDPDFLYNFLPIHKWTAADVFALHKYFGIKPNPL
                     YVQGASRVGCMNCVLSTKEEISETAARWPEHIEKHHAWEKKVRLASRWVHWMSVGTES
                     QAWMRSQLGFRIEAWMGEDEFTGEEIMRSRKYPINLGTDIKLYGLEPDIQRIEWSGFY
                     GPRGNMGAPSAIDVVEWAKTGRGGKVYDLVKASMNTAVCSSRYGLCE"
     gene            complement(1493..1714)
                     /locus_tag="M2A35_004200"
     CDS             complement(1493..1714)
                     /locus_tag="M2A35_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200423.1"
                     /translation="MSAANLPACPECGNRPEYAFKIDYQGWHRGGLKCPYDHHRVSLD
                     SPAGTRKNAEERLAPQWCELVAKIQGETE"
     gene            complement(1711..2421)
                     /locus_tag="M2A35_004201"
     CDS             complement(1711..2421)
                     /locus_tag="M2A35_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCB7200424.1"
                     /translation="METVLDALSAMGKASYREIAARLVIEPVEALNMLRDQREQGKCD
                     FEDGGWFVCGGAKSVPRPRTKNPRGEDLPPVDPVAVAALLRSKGPMTATELAVAFDRD
                     PRGMPGCLQGLALRGVVEKLGQGRRVTWSVPGYVPAEPQTEPQETAPAVPATTETAVP
                     EVAQAEATPAPEKTVAELVDTIPTFVGRPDDLLIPTVRSISNEIRRTKSKLAALENLR
                     DSVRTIRRYQHIAREISQ"
     gene            complement(2464..3390)
                     /locus_tag="M2A35_004202"
     CDS             complement(2464..3390)
                     /locus_tag="M2A35_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200425.1"
                     /translation="MSVKLSAWVWDGCAAAGIKGNKLLIMLRLADYASDEGIAYPSVA
                     TIARQLGAGRSTVIGLINELVRDGWLTKRERRQGQRSTSNLYTLNVSRLRRAAAEAYA
                     GGPDSGRPETEPPETERPENPKKPGSEGPESGHDPSVNSKQDPSVEKPISPDATQPDA
                     ERGDADELLNGHPEAVIFSAKKRQWGSREDLTCAEWIWGKVVRLYERAAESDGEVVRP
                     REPNWAAWANEVRLMCNQDGRTHRQICELFERANRDPFWCKNILSPGKLREKWDDLSL
                     KLAAYSVPREDQNFRASYDDVDYSKVPDGFRV"
     gene            complement(3403..3816)
                     /locus_tag="M2A35_004203"
     CDS             complement(3403..3816)
                     /locus_tag="M2A35_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200426.1"
                     /translation="MTGLDRAIKIIGNQRRLALALNVSAMSVNDWVHKYKGRVPSGRV
                     QGIFQITGVTPHELRPDLYPNPTDGLTSSAEAAPVRQGVSEQVGESVRECAEAHCAVL
                     TNAPPEIQEREALQAIAALVPFVPGHRVLILPREQ"
     gene            3924..4307
                     /locus_tag="M2A35_004204"
     CDS             3924..4307
                     /locus_tag="M2A35_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200427.1"
                     /translation="MKKTTYDTPLSARLYEIATRNHWNDADMGRIADVSRSSVYGWHK
                     RGTISKAAAAKIAAAANVSLDWLLTGKDEKTAGALSEDERALVDVYRALPPIEQRNML
                     AAFQMRLEALRDYYANNVDPVTRQK"
     gene            4314..4667
                     /locus_tag="M2A35_004205"
     CDS             4314..4667
                     /locus_tag="M2A35_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200428.1"
                     /translation="MPAKYAAFAERLTAEMQKRRVTVKELSAVCSVTYEMARRYTLGT
                     ARPRSDKLSALAEYLIVSPAWLDYGTESPDLTTLTDDEIRLLRAFRQLPYSEAIAALH
                     QLEQQYQTSIKRRDK"
     gene            4890..5093
                     /locus_tag="M2A35_004206"
     CDS             4890..5093
                     /locus_tag="M2A35_004206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCB7200429.1"
                     /translation="MIGNYGTKQEHSSTVAPGTLLRHNGMTWRASANTGGNLYLLRPF
                     EQKRVSNCIVDVFLNERGKPQIQ"
     gene            5170..5895
                     /locus_tag="M2A35_004207"
     CDS             5170..5895
                     /locus_tag="M2A35_004207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200430.1"
                     /translation="MDEQNKNQLNDADINILARQSIGEVLAGRIAAFSTSPKMLQIID
                     SKIESCVNGIVNDVFSNYSDFSKSTKEAFRAALPGNMENVIDLGRYNDMITQRLRETF
                     AGSGVADSMIVKAEKMLKEVMDESLLPPVIKLSEFLDAFIDANVEQAAENGWERPDIR
                     LIDSEYSMLSHDYKSLYFDKSPDPESRIHGLRSQYRLDNNISMRALEGETIDGHQVYE
                     VYSAKIDERHVQHIVSTNLIKSR"
     gene            6220..6450
                     /locus_tag="M2A35_004208"
     CDS             6220..6450
                     /locus_tag="M2A35_004208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200431.1"
                     /translation="MVILETESEDRLMLNRLAVIMHGSVTDINLLPVTAVALVERCKR
                     LERERDTLARDRDELVSSIVGSVEALLKQYQQ"
     gene            6492..6737
                     /locus_tag="M2A35_004209"
     CDS             6492..6737
                     /locus_tag="M2A35_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCB7200432.1"
                     /translation="MIQMLTLEEWRADKYRSNPPSLDTLRRYAKQSMFNPPARKEGKY
                     WRVREDAEITGSIAQPVIRKSDPIMLQRILNDGCQTT"
     gene            6718..7845
                     /locus_tag="M2A35_004210"
     CDS             6718..7845
                     /locus_tag="M2A35_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCB7200433.1"
                     /translation="MAARPRKNNVKIPNLYPLYSRKSKKIYWRYKHPITGKFHALGTD
                     ETVATEIAIEANRRIAEQHARQVQAITDLISTSKGGAITVNTWLDRYWKIQDERLKID
                     DIKVNTHKQKLKPVNLLREAAGMRLITDVTVRDIAQILDQYLSAGQPRMAQVIRSVLI
                     DVFKEAQHAGEVPPGHNPALATKQPRRRITRQRLNLEEWQKIFDIADATHKYLGNAML
                     LAVVTGQRLGDISRMKFTDIWDDHLHVVQEKTGSKVAIPLALRCNAINWSLREVVARC
                     RDYAVSPYLVHFFRATSQAERGAQVKANTLTMNFSKARDQAALDWGDGTPATFHEQRS
                     LAERLYRDQGIDTKELLGHKSQRQTDRYHDDRGKDWIKVSV"
     gene            complement(7889..8578)
                     /locus_tag="M2A35_004211"
     CDS             complement(7889..8578)
                     /locus_tag="M2A35_004211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200434.1"
                     /translation="MKIRKYLPICIALVLTGCKVELTTIVNMDDVLSREHKEVTADLN
                     FEVAACNDYEDSRKESQILTQVKEQIPGIFDGAKYVECYNKKLNSYAHFRVPVDVGAI
                     YENMVLANPNADIFVTSKIDDGNIAVLALSDKVRAKLDKAKKSAATDFDISVTIIHPS
                     KPIKFVTQGIFVTNSREVKTPIDSGSLDWNTPTDMTFTLSDVGRASLFKNGVHEILMT
                     EARAIDRIKLK"
     gene            complement(8819..10069)
                     /gene="icd"
                     /locus_tag="M2A35_004212"
     CDS             complement(8819..10069)
                     /gene="icd"
                     /locus_tag="M2A35_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCB7200435.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWVEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            10241..10894
                     /gene="rluE"
                     /locus_tag="M2A35_004213"
     CDS             10241..10894
                     /gene="rluE"
                     /locus_tag="M2A35_004213"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCB7200436.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            10904..11365
                     /gene="nudJ"
                     /locus_tag="M2A35_004214"
     CDS             10904..11365
                     /gene="nudJ"
                     /locus_tag="M2A35_004214"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCB7200437.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            11419..12525
                     /gene="mnmA"
                     /locus_tag="M2A35_004215"
     CDS             11419..12525
                     /gene="mnmA"
                     /locus_tag="M2A35_004215"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCB7200438.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            12561..13202
                     /gene="hflD"
                     /locus_tag="M2A35_004216"
     CDS             12561..13202
                     /gene="hflD"
                     /locus_tag="M2A35_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCB7200439.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            13206..14576
                     /gene="purB"
                     /locus_tag="M2A35_004217"
     CDS             13206..14576
                     /gene="purB"
                     /locus_tag="M2A35_004217"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCB7200440.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFVADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            14744..15415
                     /gene="phoP"
                     /locus_tag="M2A35_004218"
     CDS             14744..15415
                     /gene="phoP"
                     /locus_tag="M2A35_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCB7200441.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARIQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            15415..16875
                     /gene="phoQ"
                     /locus_tag="M2A35_004219"
     CDS             15415..16875
                     /gene="phoQ"
                     /locus_tag="M2A35_004219"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCB7200442.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            16951..18072
                     /gene="roxA"
                     /locus_tag="M2A35_004220"
                     /gene_synonym="ycfD"
     CDS             16951..18072
                     /gene="roxA"
                     /locus_tag="M2A35_004220"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCB7200443.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
BASE COUNT         4487 a         4699 c         4538 g         4443 t
ORIGIN      
        1 ctgacgctgt acgcggcggt tatgcgcagc cctcacctcc tgccagcgtt gctctggcat
       61 gtttttataa ttgctgcggc agcggtcctt ctttgcctga ttttcgtttt tatatgcctg
      121 gcttaaatct tttaaatacg tatatttact cattattcca cttcctgaat atgttcctat
      181 tcgcataatc cgtaacgtga agagcacacg gcagtgttca tgctggcctt caccagatca
      241 taaacctttc cgccgcgccc ggttttcgcc cactcaacaa catcaatcgc tgacggcgca
      301 cccatattcc cgcgcggccc gtagaatcct gaccactcaa tgcgctgaat gtctggctct
      361 aagccgtata gtttgatatc cgtaccgagg tttattggat atttcctgct ccgcataatt
      421 tcctctccgg taaattcatc ctctcccatc caggcttcga tgcgaaatcc caactgtgac
      481 cgcatccatg cctgactttc cgttccgaca ctcatccaat gcacccagcg tgatgccagg
      541 cgtactttct tctcccatgc atggtgtttt tcgatatgct caggccagcg tgcagccgtc
      601 tcagaaattt cctctttggt gctcaacacg cagttcatgc aaccgacacg ggacgcaccc
      661 tgcacgtaca gaggatttgg tttaataccg aaatatttat gcaacgcaaa aacatctgcc
      721 gctgtccatt tatgaatagg caggaagtta tagaggaaat ctggatcacg ctcgtcacgc
      781 gcaaatctcg aataaccggc gcgcttgtca gactcatctg cgcgaacgcc agaccactga
      841 acgacgacct ctccatcatc caaaagtggt tttatcgcag cgtcaaaagc gatctgaatt
      901 ttcagttcgt cagtacaaaa acggtctctc agcattggaa atttgccatg caataacgca
      961 gcatccagga agctgtttcc gctcggatgc atcactgaaa tagcggcctc cagaggtgtc
     1021 tcaaactcaa tcccccagcg ctcagcggtg cgtaaccatg cctgaccaaa tttggtatct
     1081 gcccgcgcca acgatggcat gattacacct cgatatgccc ccattctaat tgcctggcgt
     1141 ttaggccagc ggtctttgag agactggcga cggcgctcaa actccgcctc ggtgtaaatg
     1201 cgcttaacaa tttgaacagg tttacaaccg atttgatgat ggacattacg agcaaaatcg
     1261 acagttaatt catgctcgtt atcagtatcc gccataacat ttttaacacg gtcgccgaac
     1321 aggtgatgcg caatggtcgc tgttgtagtg ctgtcttttc ctgcggagaa attcacaacg
     1381 attttgtgat cgtccgggat gcggaattct tcgagatacc ggtaataagc cgtctctacc
     1441 tcacgcacca tctcgcgaat atctgttggc gcaatgatca tcgctgcgtc gctcattctg
     1501 tctctccctg tatttttgcg accagctcac accactgtgg ggccagcctt tcctctgcgt
     1561 ttttgcgcgt acccgccggg ctgtcgagtg atacgcggtg gtggtcgtag gggcatttca
     1621 gcccgccacg gtgccagccc tggtagtcaa ttttgaatgc gtattcaggt ctgtttccgc
     1681 actccgggca ggcgggtaaa ttcgcggcgc tcattgtgaa atctcccggg caatatgctg
     1741 atagcgtcga attgtgcgca ctgaatcgcg cagattttcg agcgcagcga gttttgattt
     1801 agttcgtcgt atttcgttag agatactacg cacagtgggg atcagcaggt catccgggcg
     1861 accgacgaac gtcgggatag tgtccaccag ctccgctacc gttttttctg gtgccggggt
     1921 ggcttctgcc tgcgcgacct ccggtactgc ggtttctgtt gttgctggca ctgctggtgc
     1981 tgtttcctgc ggctctgttt gcggttccgc gggtacgtaa cccggcacag accaggtaac
     2041 gcggcggccc tgcccgagct tttcgaccac accccgcagc gccagcccct gcaaacaacc
     2101 aggcattccg cgtggatcac ggtcgaacgc aacagccagt tcagttgcgg tcatcggccc
     2161 cttgctgcgc aacagcgccg ccaccgccac tggatcgact ggcggcaaat cctcgccgcg
     2221 ggggttttta gtgcggggcc gtggcactga tttagctccg ccgcagacaa accagccgcc
     2281 gtcctcaaaa tcgcatttgc cctgttcccg ttggtcgcgc agcatgttta acgcttctac
     2341 cggttcaatc accaggcggg ctgcgatttc gcgatagctc gccttgccca tcgctgatag
     2401 cgcatcaagt acagtttcca taaattcacc tcgtttatat atttgaatca attgattggc
     2461 gtgctacacc ctgaacccgt caggcacttt gctgtaatcc acgtcgtcgt agctcgctct
     2521 aaaattctgg tcctcgcgcg gcacgctgta tgcggcgagt tttagcgaca ggtcatccca
     2581 tttttcgcgg agctttcccg gtgacagaat gtttttgcac cagaacggat cgcggtttgc
     2641 ccgttcgaac aactcgcaga tctggcgatg cgtcctgccg tcttggttgc acatcagccg
     2701 cacctcgttt gcccaggccg cccagttcgg ctccctcggt cgtacaacct cgccgtctga
     2761 ctcagcggcc cgctcgtaca gcctcacgac tttcccccaa atccattccg cacaggtcag
     2821 gtcctcacgg ctcccccact ggcgcttttt cgcactgaaa atcacagcct ccgggtgacc
     2881 gtttaaaagt tcatctgcat caccccgttc ggcgtccggt tgcgtagcgt ccggactaat
     2941 aggtttttct actgacggat cttgttttga atttactgac ggatcgtgtc cagattctgg
     3001 accctcagaa ccgggttttt tggggttttc tggacgttca gtttctggcg gttcagtttc
     3061 tggacgtcca gaatctggac ccccggcgta tgcctcggcg gcggctctgc gcaaccgcga
     3121 aacattcagc gtatagagat tactggtgct gcgttgaccc tgacggcgct ctcgttttgt
     3181 tagccagccg tcgcgcacca gttcattgat taacccgatc acggtgctgc ggcccgctcc
     3241 cagttgccgt gcaatcgttg ccacgctcgg ataggcgatc ccctcgtcgc tcgcgtaatc
     3301 agccaggcgc agcatgatca gcagcttgtt gcctttgatg cccgccgccg cgcaaccgtc
     3361 ccaaacccac gcggataatt tcacgctcat gcgatactcc gcttattgct cacgcggcag
     3421 gattaacacc cggtgaccag gcacaaacgg gaccagcgct gctattgcct ggagggcctc
     3481 gcgctcctga atctcgggtg gcgcgtttgt gagcactgcg caatgagcct cggcacactc
     3541 gcgcactgat tcgcccacct gctcactcac cccctgacgt accggtgctg cctctgccga
     3601 gctggtcagg ccgtccgttg gattcgggta caaatccggg cgcagctcgt gcggagtaac
     3661 gccggtaatt tggaaaattc cctggacccg ccctgatgga acgcggccct tgtatttatg
     3721 cacccagtcg tttaccgaca tggcgctcac gtttagcgcc agagccaggc ggcgctggtt
     3781 gccgataatt tttatagctc tatctaaacc cgtcatgatc gttccccttg taaatccgtg
     3841 actgacattg tcagctacaa tcttacagcg tgtcaacagg aatataaatt gtttctatca
     3901 ggtaaagccg tataattgcc cgtatgaaaa aaacaacgta cgacaccccg ctatctgcgc
     3961 ggctttacga gatagcgacg cgcaatcact ggaatgatgc cgacatgggc aggattgccg
     4021 acgttagtcg gtcgtcggtt tacggctggc acaaacgagg cactatcagt aaggcggctg
     4081 ccgccaaaat cgcagccgcc gccaatgttt ctctggactg gctactcacg gggaaagacg
     4141 agaaaactgc gggcgctctc agcgaggatg aacgggcgct cgttgacgta tacagagcat
     4201 tacccccgat cgagcagcgc aacatgctcg cagcgtttca aatgaggctg gaggcattac
     4261 gcgattatta cgctaacaat gttgatccag tgacccgcca gaaataacaa tttatgcccg
     4321 ctaaatatgc cgcgttcgct gaacggctca ctgccgaaat gcagaaaagg cgcgtaaccg
     4381 ttaaagagtt aagcgccgtc tgttctgtta cgtatgagat ggcgcggcgg tacaccctcg
     4441 gcaccgcgcg tccgcgaagt gataaattgt ccgccctggc ggaatacctc atcgtctccc
     4501 cagcctggct ggattacggc acagaatcac cagatctaac cactctcact gacgatgaga
     4561 tccggttact ccgcgcgttc cgtcagctcc cctattctga ggctatagca gcactccacc
     4621 agctcgagca gcagtatcag acgtctatta aacgtcgcga caaataatct cagcacacct
     4681 aaaaagcccc tccagcaaaa aaataatggc gtaaaaacgt ttagatttta tgcgcttatg
     4741 ctccaccagc aggaatgcca gcaaaaaata tgtaagcccg cgattgacac aagtcagtca
     4801 ataactgaca ctgtatacat cgaagcacac cggtgcgacg ggaaaacgtt cagcggcccg
     4861 gcttaagggc aaaaattttt gggggtctta tgattggtaa ctacggcaca aaacaggagc
     4921 acagcagcac cgttgcacct ggcacactgt tacgccacaa cggcatgacc tggcgcgcgt
     4981 cagcaaatac cggcggcaat ctgtatttac tccgcccgtt tgaacaaaaa cgggtatcta
     5041 actgcatcgt tgatgtgttt ttaaacgagc gcggcaaacc acaaattcag taatcagcaa
     5101 cactgcgaat tattacgcca ctcgttggcg gggatttctg caacctaaat ttaatcagga
     5161 gcaatttaaa tggacgaaca aaataaaaac caactgaatg atgccgatat taatatttta
     5221 gcacgtcaga gtattggcga ggtattagcg gggcgtattg ctgcgttttc aactagccca
     5281 aaaatgctgc aaatcatcga ctccaaaatc gaatcgtgcg ttaacggtat cgttaatgat
     5341 gttttctcta attacagtga tttttcaaaa tccactaaag aggcgttccg cgcagccctg
     5401 ccaggcaata tggaaaatgt catcgacctg gggcgataca acgacatgat cacacaacgc
     5461 ctgcgcgaaa cgttcgccgg aagcggtgtt gctgattcaa tgatagtcaa ggccgagaaa
     5521 atgcttaaag aagtcatgga cgaatcgctg ttaccaccgg ttattaaatt aagcgaattt
     5581 ttagacgcct ttattgatgc taacgtagaa caggcggcag agaatggctg ggaacgccca
     5641 gacatacgac tgattgacag cgaatattca atgttaagcc atgactataa aagcttatat
     5701 tttgataaat cgccagatcc tgaatcacga attcacggcc tgcgcagtca atatcgcctc
     5761 gataacaata tcagcatgcg cgctctggag ggtgaaacaa ttgatggtca ccaggtgtat
     5821 gaagtttata gcgccaaaat tgacgaaaga catgttcagc acattgtaag taccaattta
     5881 attaaatcgc gctgaaaaaa aatggttttt gcgctttatt acggtcagtc taaaatatgc
     5941 atcgattgcg accctgacta ttattactat ccgtgtaatg actaatgact caggatcagg
     6001 aaaacgcgtt acgagctgta gcgcgtcaat gtaacgagga attaaaagcg gcagtcaggc
     6061 gggaaccaaa acccaaattt gatgacgtta gccgcccaat attagctaaa cattacgaga
     6121 aaataaaacc gctcggaatt ccgtttattc gatttgtctg gacaattgga gtattaaacg
     6181 ggcggtttat agagcgttaa tttaaacgag gaatagtgaa tggtaattct ggaaacagaa
     6241 agcgaggatc ggctcatgct caatcgcctg gctgtaataa tgcacggcag cgtgactgat
     6301 attaatttat tacctgtaac tgccgtagct ctggtagaac gctgcaaacg actggagcga
     6361 gagcgcgaca cactggcgcg ggatcgcgat gaactggtta gcagtatcgt ggggagcgta
     6421 gaagctctgt taaaacagta tcagcaataa taacgtcccg cccgttcgtg ggcggtctgg
     6481 agcaaaaaac catgatccaa atgttaactc ttgaggaatg gcgtgcagac aagtaccgga
     6541 gcaatccccc aagcctggac acgttgcgcc ggtacgcaaa gcaaagcatg tttaatccac
     6601 cagccagaaa agaggggaaa tattggagag ttagggagga tgccgaaatt actggcagca
     6661 tagcccagcc agtgattaga aaatcagacc cgataatgtt acagaggatt ttaaacgatg
     6721 gctgccagac cacgtaaaaa caacgtaaaa atccctaacc tgtaccctct gtatagccgg
     6781 aaatctaaaa aaatttattg gcgatacaag cacccgatta ccggcaaatt tcatgcactc
     6841 ggcactgatg aaacggtcgc tacggaaatc gcaattgagg cgaacaggcg gatcgctgaa
     6901 cagcacgcca ggcaagtcca ggcgattaca gacctcattt ccaccagcaa aggcggcgct
     6961 attactgtta acacctggct ggaccgatac tggaaaatac aagacgagcg tttaaagatc
     7021 gatgatatta aggtcaatac gcacaaacaa aaacttaagc cggtgaatct attacgtgag
     7081 gccgctggca tgaggttaat aacagatgtc acggttcggg acatagcgca gattctggac
     7141 caatatcttt ctgcgggcca gcccagaatg gcgcaggtta tccgctctgt gctcattgac
     7201 gttttcaaag aggctcaaca cgctggcgag gttccgccag ggcacaaccc tgcactggca
     7261 actaaacagc ctcggcgcag aattacccgg caacgcctga acctggaaga atggcagaag
     7321 atttttgata ttgccgacgc cactcacaaa tacctcggca acgccatgct acttgctgtc
     7381 gtgacaggtc agcgattagg cgatatatca agaatgaaat ttactgatat ttgggatgac
     7441 catctgcatg tagtgcagga aaaaacaggg agtaaggtcg caatcccact ggcgctccgt
     7501 tgtaatgcaa tcaattggag tttgcgcgaa gttgtcgccc gctgccgtga ttatgccgtc
     7561 agcccttacc tcgtgcattt ttttcgcgca acttcgcaag cggaacgcgg ggcacaggtg
     7621 aaggctaaca cgctaacgat gaattttagc aaggcgcgcg accaggctgc acttgactgg
     7681 ggcgacggca ctccagccac atttcatgaa caaagatcac tagcagaacg actctaccgc
     7741 gatcagggta ttgatactaa agaattgctg gggcacaaat cgcaaaggca aacggaccga
     7801 taccacgatg atcgcggaaa ggactggatt aaagtatcag tttaaagtta gccaaacagg
     7861 tcgacatgtt tggctaacag ctaacttatt acttcaattt aatacgatct attgctcgcg
     7921 cttctgtcat caatatttca tgcaccccgt ttttgaaaag tgacgccctt ccaacatcag
     7981 ataacgtaaa cgtcatatct gtaggagtat tccaatccag agagcctgaa tcaattggtg
     8041 ttttcacttc acggctatta gtaacaaaaa taccctgtgt gacaaattta attggtttgc
     8101 taggatgaat aattgttacg cttatatcaa aatctgtcgc tgcagatttc tttgccttat
     8161 ctaattttgc tctcacttta tcggaaagag ccaacacagc gatattccca tcatctattt
     8221 tcgaagtcac gaaaatatcc gcatttgggt tagccagtac catattttca taaatagccc
     8281 ctacatcaac cgggacgcga aaatgtgcgt aggaatttaa ttttttattg tagcactcga
     8341 catatttggc cccgtcaaat atgccgggga tctgttcttt aacctgcgtc aggatttgtg
     8401 actctttccg cgagtcttca taatcattgc atgctgccac ctcaaaatta agatcggcgg
     8461 tgacttcctt atgttctctg gataggacat cgtccatatt aacaatcgtg gtgagttcaa
     8521 ctttgcatcc agtgagaacc agcgcaatgc agatcgggag gtattttcga attttcatgt
     8581 gcgtatcctt gcaaaaaaag aggtccgatt ttctatctac atagctccac tcaatcgtga
     8641 tcatgcgcat agttatcttg atatgttaat ttttcagata accgcccgac ttgttcaagg
     8701 acgctttagg gaggggtttt gataatttgt tttgataaaa ttttgataac cgttagcagg
     8761 ctaacaaaaa acaacgggag cgttacaccc ccgttaatgg atttaacaaa tcacggtatt
     8821 acatgttctt gatgatcgcg tcaccaaact ctgaacattt cagcagctta gcgccttcca
     8881 tcagacgttc gaagtcatag gttacggtct tggcgttgat tgcgccttcc atacctttaa
     8941 caattaagtc agccgcttca acccaaccca tatggcgtaa catcatctca gcggagagaa
     9001 taatagagcc agggtttact ttgtcctgac cggcgtattt cggcgcagta ccgtgggtgg
     9061 cttcaaacag ggcgcattcg tcaccgatgt ttgcaccagg ggcgataccg ataccgccaa
     9121 cctgcgctgc cagggcgtca gaaatgtagt caccgttcag gttcatacag gcgataacat
     9181 catattcagc cggacgcagc aggatctgtt gcaggaatgc atcagcaatc acgtctttaa
     9241 tgacgatctc tttgccggtg ttcgggtttt taactttcag ccacgggccg ccgtcgatca
     9301 gttcaccgcc aaactcttca cgcgccagct ggtagcccca gtctttaaac gcgccttcgg
     9361 tgaacttcat gatgttgcct ttgtgcacca gggtcacaga gtcacggtcg ttggcaattg
     9421 cgtattcaat cgcggcacgg accagacgtt tggtgccttc ttcagaacac ggcttgatac
     9481 cgataccgca atgttccggg aagcgaattt tcttcacgcc catctcttca cgcaggaatt
     9541 taatcacttt ctcggcgtcg gcagagtctg ctttccattc gatacccgca taaatgtctt
     9601 ccgagttttc acggaagata accatatcgg tcagttcagg gtgtttaacc gggcttggag
     9661 tgccttgata gtaacgtacc ggacgcaggc agatgtagag atccagttcc tggcgcaggg
     9721 caacgttcag agagcgaata ccgccaccaa caggagtggt cagcggacct ttaatggcaa
     9781 cgcgatattc acgaatcaga tcaagggttt cagcaggcag ccagacatcc tgaccataaa
     9841 cctgtgtgga tttttcaccg gtgtaaattt ccatccagga gattttacgc tcgcctttat
     9901 aggctttctc gactgcagcg tcgaccactt tcagcatggc tggggttaca tctacaccga
     9961 ttccatcacc ttcaatgtaa gggataatcg gattttcagg aacgttgagt ttgccgtttt
    10021 gcagggtgat cttcttgcct tgtgccggaa caactacttt actttccatt cacctctcct
    10081 tcgagcgcta ctggtttgct cgtctgccac cacgttgcat atgcgtttgc gtcctgcgat
    10141 acggatgctt tagagcaatt ttttgttaat gatttgtaat tggcttgtca atactaccct
    10201 attgtttgcc gtcatgaaag atgcgcgtta ttaggctata atgcggcaat tcataatctc
    10261 tgaaaatacc atgcaaaaaa cttcttttag aaatcaccag gttaagcgat tcagctcgca
    10321 acgttctacc aggcgtaaac ctgaaaacca gcccacgcgt gtgatcctgt tcaataaacc
    10381 ctacgatgtt cttccgcagt tcaccgatga agccggacgc aaaacattaa aagaattcat
    10441 cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc gatagcgaag ggttactggt
    10501 gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag ccgggtaaac gcaccgggaa
    10561 aatctattat gtgcaggtgg aaggtattcc cacacaagac gcacttgaag ccttgcgcaa
    10621 tggcgtaacc ttaaatgatg gccctaccct gcccgccggc gcggagctgg ttgacgaacc
    10681 agcgtggtta tggccgcgga atccgccaat tcgtgaacgc aaaagtattc ccaccagctg
    10741 gctgaagatc accttatatg aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt
    10801 tggtttcccc acgctgcgac tgattcgcta tgcgatggga gattactctt tggataatct
    10861 tgccaatggc gaatggcgag aagtgacaga ttaaggatgc aaaatgttta aaccgcacgt
    10921 taccgttgct tgcgtggtgc acgcagaagg caaattttta gtcgttgaag agacgattaa
    10981 tggtaaagcg ttatggaacc aacctgccgg gcatctggaa gccgatgaaa ccttagtgga
    11041 agccgccgcc cgtgaactgt gggaagaaac cggcatcagc gcgcagccgc aacactttat
    11101 tcgtatgcat cagtggattg cgccagataa aacgccgttt ttgcgtttcc tctttgccat
    11161 tgagcttgag caaatatgcc cgacccagcc tcatgacagc gatatcgact gctgtcgttg
    11221 ggtcagcgcc gaagagattt taaaggcgtc aaatcttcgt tcgccgctgg tggcggaaag
    11281 tatccgttgt tatcaaagcg ggcaacgcta tccgctggag atgattggcg attttaactg
    11341 gccttttaca aagggtgtca tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc
    11401 aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc
    11461 ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac agggatatca ggttgaaggc
    11521 ctgtttatga agaactggga agaagacgac ggtgaggaat attgcacagc ggcagcggat
    11581 ctggctgatg cccaggctgt ctgcgacaag ctcggcattg aactgcacac cgttaacttt
    11641 gctgccgagt actgggacaa cgtcttcgaa ctgttccttg ccgaatataa agccggtcgc
    11701 acgccgaatc cggatattct gtgcaacaaa gagatcaaat ttaaagcctt cctcgaattt
    11761 gccgccgaag atttaggtgc cgattatatc gctaccggtc attacgtacg tcgggccgat
    11821 gtcgatggca agagccgcct gctgcgtggt ctggacagca ataaagacca gagctacttc
    11881 ctttatacgc tcagccatga gcagattgcg caaagcctgt tcccggtcgg cgaactggaa
    11941 aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg tcaccgcgaa gaaaaaagac
    12001 tctaccggca tttgcttcat tggcgaacgt aaattccgcg agttcctggg ccgttatctc
    12061 ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg aaattggtga gcaccagggg
    12121 ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta tcggtggcac caaagaaggt
    12181 accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa acaacattct ggttgtcgct
    12241 cagggccatg aacacccgcg gctgatgtct gtcgggttga ttgcccagca gttgcactgg
    12301 gtcgatcgcg aaccattcac cggcactatg cgttgcacgg taaaaacccg ctatcgccag
    12361 accgatatcc cttgcaccgt caaggcgctg gacgatgatc gcattgaagt gattttcgat
    12421 gaaccggttg ccgccgtgac gccgggccag tctgccgtct tctataacgg tgaagtgtgc
    12481 ctcggtggcg gtattattga gcagcgtctg ccgctgccgg tctgattatt atctttactt
    12541 aaaacaagga agcagtgaac gtggcaaaga attactatga catcaccctc gccctggccg
    12601 gtatttgtca gtcggcacgc ctggtgcaac aactcgctca ccaggggcat tgtgatgccg
    12661 atgcgctaca cgtctcactc aacagtatta ttgatatgaa ccccagctcg acgctggcgg
    12721 tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga aaccctgctc ggcgtgctca
    12781 atgccagcag tcgccagggc ttaaacgccg aattaacccg ctacacactc agcttgatgg
    12841 tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga cactctgggc aaccggatca
    12901 acggcctgca acgccagctc gaacacttcg atttacagtc cgaaacgctg atgagcgcga
    12961 tggctgctat ctatgttgat gtgatcagcc cgcttggccc gcgcattcag gtcaccggtt
    13021 cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag
    13081 gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg acgtctgcaa ctgatgtttt
    13141 ctcgtaatcg cctgaccact caggcaaaac aaattcttgc tcatttaacc ccggagttgt
    13201 gatctatgga attatcctca ctgaccgccg tttcccctgt cgatggacgc tacggcgata
    13261 aagtcagcgc gctgcgcggg attttcagcg aatatggttt gctgaaattc cgtgtacaag
    13321 ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc gatcaaggaa gttcctgctt
    13381 ttgttgccga cgcaatcggt taccttgatg caattgtcgc caatttcagc gaagaagatg
    13441 ccgcacgcat caaaaccatc gagcgtacca ctaaccacga cgttaaagcg gttgagtatt
    13501 tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc ggtttctgaa ttcatccact
    13561 ttgcctgtac ttcggaagat atcaataacc tctcccacgc attaatgctg aaaaccgcgc
    13621 gtgatgaagt gatcctgccg tactggcgtc aactgattga tggcattaaa gatctcgccg
    13681 ttcagtaccg cgatatcccg ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa
    13741 ccatcggtaa agagatggca aacgtcgcct accgtatgga gcgccagtac cgccagctta
    13801 atcaggtgga gatcctcggc aaaatcaacg gcgcggtcgg taactataac gcccatatcg
    13861 ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta
    13921 ttcagtggaa cccgtacacc acccagatcg aaccgcacga ctacattgcc gaattgtttg
    13981 attgcgttgc gcgcttcaac accattctga tcgactttga ccgtgacgtc tggggttata
    14041 tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga gattggttct tccaccatgc
    14101 cgcataaagt taacccgatt gacttcgaaa actccgaagg gaatctgggc ctttccaacg
    14161 cggtattgca gcatctggca agcaaactgc cggtttcccg ctggcagcgt gacctgaccg
    14221 actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta tgcgctgatt gcgtatcaat
    14281 ccaccctgaa aggcgtgagc aaactggaag taaaccgtga ccatctgctg gatgagctgg
    14341 atcacaactg ggaagtgctg gctgagccaa tccagacagt tatgcgtcgc tatggcatcg
    14401 aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa gcgcgttgac gccgaaggca
    14461 tgaagcagtt tatcgatggt ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcga
    14521 tgacgccggc taactatatt ggtcgagcta tcaccatggt tgatgagctg aaataaactc
    14581 gtatcagtgc cggatggcga tgctgtccgg cctgtttatt aaaattatcc actttttctt
    14641 ttttcacttt acctcccctc cccgctggtt tatttaatgt ttacccccat aaacacataa
    14701 tcgcgttaca ctattttaat aattaagaca gggagaaata aaaatgcgcg tactggttgt
    14761 tgaagacaat gcgttgttac gtcaccacct taaagttcag attcaggatg ctggtcatca
    14821 ggtcgatgac gcagaagatg ccaaagaagc cgattattat ctcaatgaac atttaccgga
    14881 tattgcgatt gtcgatctcg gattgcctga cgaggacggt ctgtcactga ttcgccgctg
    14941 gcgtagcaat gatgtttcac tgccgattct ggtattaacc gcccgtgaaa gctggcagga
    15001 caaagtcgaa gtattaagtg ccggtgctga tgattacgtg actaaaccgt ttcatattga
    15061 agaggtgatg gcgcgaattc aggcattaat gcggcgtaat agcggcctgg cttcacaggt
    15121 catttcgcta cccccgtttc aggttgatct ctctcgccgt gaattatcta ttaatgacga
    15181 agtgatcaaa ctgaccgcgt tcgaatacac cattatggaa acgttgatac gcaataatgg
    15241 caaagtggtg agcaaagatt cgttaatgct ccaactctat ccggatgcgg agctgcggga
    15301 aagccatacc attgatgtac tgatgggacg tctgcgcaaa aaaattcagg cacaatatcc
    15361 gcaagaagtg attactaccg ttcgcggcca gggctatttg ttcgaattgc gctgatgaaa
    15421 aaattactgc gtcttttttt cccgctctca ctgcgggtac gttttctgtt ggcaacggct
    15481 gcggtagtac tggtgctttc gcttgcctac ggaatggtcg cgctgatcgg ttatagcgtc
    15541 agtttcgata aaactacgtt tcggctgtta cgtggcgaga gcaatctgtt ctataccctt
    15601 gcgaagtggg aaaacaataa gttgcatgtc gagttacccg aaaatatcga caagcaaagc
    15661 cccaccatga cgctaattta tgatgagaac gggcagcttt tatgggcaca acgtgacgtg
    15721 ccctggctga tgaagatgat ccagcctgac tggctgaaat cgaatggttt tcatgaaatt
    15781 gaagcggatg ttaacgatac cagcctcttg ctaagtggag atcattcgat acagcaacag
    15841 ttgcaggaag tgcgggaaga tgatgacgac gcggagatga cccactcggt ggcagtaaac
    15901 gtctacccgg caacatcgcg gatgccaaaa ttaacctttg tggtggtgga taccattccg
    15961 gtggagctaa aaagttccta tatggtctgg agctggttta tctatgtgct ctcagccaat
    16021 ctgctgttag tgatcccgct gctgtgggtc gccgcctggt ggagtttacg ccccatcgaa
    16081 gccctggcaa aagaagtccg cgaactagaa gaacataacc gcgaattgct caatccagcc
    16141 acaacgcgag aactgaccag tctggtacga aacctgaacc gattgttaaa aagtgaacgc
    16201 gaacgttacg acaaataccg tacgacgctc accgacctga cccatagtct gaaaacgcca
    16261 ctggcggtgc tgcaaagtac gctgcgttct ctgcgtagtg aaaagatgag cgtcagtgat
    16321 gctgagccgg taatgctgga gcaaatcagc cgcatttcac agcaaattgg ctactacctg
    16381 catcgtgcca gtatgcgcgg cgggacattg ctcagccgcg agctgcatcc ggtcgcccca
    16441 ctgctggaca atctcacctc agcgctgaac aaagtgtatc aacgcaaagg ggtcaatatc
    16501 tctctcgata tttcgccaga gatcagcttt gtcggtgagc agaacgattt tgtcgaggtg
    16561 atgggcaacg tgctggataa tgcctgtaaa tattgcctcg agtttgtcga aatttctgca
    16621 aggcaaaccg acgagcatct ctatattgtg gtcgaggatg atggccccgg tattccatta
    16681 agcaagcgag aggtcatttt cgaccgtggt caacgggttg atactttacg ccctgggcaa
    16741 ggtgtagggc tggcggtagc ccgcgaaatc accgagcaat atgagggtaa aatcgtcgcc
    16801 ggagagagca tgctgggcgg tgcgcggatg gaggtgattt ttggtcgcca gcattctgcg
    16861 ccgaaagatg aataaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
    16921 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
    16981 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
    17041 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
    17101 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
    17161 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtgaa ccactggcat
    17221 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
    17281 ctgatgatct ctttttctgt accgggcggc ggcgtcggcc cgcatctcga tcagtacgac
    17341 gtgtttatca ttcagggtac cggtcgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
    17401 aaacagcact gtccgcaccc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
    17461 gaagagctgg agcctggcga tattctttat attccgccag gattcccgca tgaaggctac
    17521 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
    17581 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
    17641 ccggatgttc cacctcgcgc tcatcctgcg gacgttctgc cgcaagagat ggataaactg
    17701 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
    17761 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
    17821 gatgaaatct acgatgcgct gaaacaaggt gaagtgctgg tgcgcctggg tggtctgcgc
    17881 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
    17941 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
    18001 ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc
    18061 ttcgaagggt aagtttggat ttaagccgga tgcggcgctg attgcgcctt atcgtgccta
    18121 catgcactga acgcctgtag gcacgataag tatgttgacc agtatca
//
LOCUS       DAHBHC010000100        17459 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHBHC010000100 DAHBHC010000000
VERSION     DAHBHC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.101"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(75..938)
                     /locus_tag="M2A35_004221"
     CDS             complement(75..938)
                     /locus_tag="M2A35_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCB7200444.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(910..2460)
                     /locus_tag="M2A35_004222"
     CDS             complement(910..2460)
                     /locus_tag="M2A35_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCB7200445.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2718..3497
                     /gene="deoC"
                     /locus_tag="M2A35_004223"
     CDS             2718..3497
                     /gene="deoC"
                     /locus_tag="M2A35_004223"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCB7200446.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3624..4946
                     /gene="deoA"
                     /locus_tag="M2A35_004224"
     CDS             3624..4946
                     /gene="deoA"
                     /locus_tag="M2A35_004224"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCB7200447.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            4998..6221
                     /gene="deoB"
                     /locus_tag="M2A35_004225"
     CDS             4998..6221
                     /gene="deoB"
                     /locus_tag="M2A35_004225"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCB7200448.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6300..7019
                     /gene="deoD"
                     /locus_tag="M2A35_004226"
     CDS             6300..7019
                     /gene="deoD"
                     /locus_tag="M2A35_004226"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCB7200449.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7294..7443)
                     /locus_tag="M2A35_004227"
     CDS             complement(7294..7443)
                     /locus_tag="M2A35_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200450.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(7475..8491)
                     /gene="lplA"
                     /locus_tag="M2A35_004228"
     CDS             complement(7475..8491)
                     /gene="lplA"
                     /locus_tag="M2A35_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCB7200451.1"
                     /translation="MSTLRLLISDSYGPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(8519..9163)
                     /locus_tag="M2A35_004229"
     CDS             complement(8519..9163)
                     /locus_tag="M2A35_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCB7200452.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            9269..10237
                     /gene="serB"
                     /locus_tag="M2A35_004230"
     CDS             9269..10237
                     /gene="serB"
                     /locus_tag="M2A35_004230"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCB7200453.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLHS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10286..11668
                     /gene="radA"
                     /locus_tag="M2A35_004231"
     CDS             10286..11668
                     /gene="radA"
                     /locus_tag="M2A35_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCB7200454.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            11689..12921
                     /gene="nadR"
                     /locus_tag="M2A35_004232"
     CDS             11689..12921
                     /gene="nadR"
                     /locus_tag="M2A35_004232"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCB7200455.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13228..14895)
                     /gene="ettA"
                     /locus_tag="M2A35_004233"
     CDS             complement(13228..14895)
                     /gene="ettA"
                     /locus_tag="M2A35_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCB7200456.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            15106..17043
                     /gene="sltY"
                     /locus_tag="M2A35_004234"
     CDS             15106..17043
                     /gene="sltY"
                     /locus_tag="M2A35_004234"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCB7200457.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17133..17459
                     /gene="trpR"
                     /locus_tag="M2A35_004235"
     CDS             17133..17459
                     /gene="trpR"
                     /locus_tag="M2A35_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCB7200458.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         4022 a         4556 c         4842 g         4039 t
ORIGIN      
        1 atgcctgatg cgacgcttgt cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg
       61 ccgtatccgg catgtcacaa atagagcgcc ggaaatatca accggctcac cccgcgcacc
      121 tttaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc
      181 tcgccataca cgccgtgggc atgaaacgcg ttcaggcgta ccggaacatc gccgagtccc
      241 ttgataaacg ccgccagttc ttcgatgtgt tgcaaataat ccacctggcc agggatcacc
      301 agcaaacgca gttccgccag cttaccgcgc tctgccagca aacagatgct gcgcttaatc
      361 tgctgattat cgcgtccggt tatttgttga tgacattcgc taccccatgc tttgagatcg
      421 agcattgcgc cgtcgcacac cgggagcaat ttttcccagc cggtctcgct caacataccg
      481 ttactgtcca ccagacaggt gagatggcgc agttgcggat catttttgat agcagtaaac
      541 agcgccacca caaacggcag ctgggtcgtg gcttcgccgc cactcaccgt tatcccttcg
      601 ataaacagca ccgctttgcg gacatggcta agcacttcgt ccacgctcat ggattgcgcc
      661 atcggcgtgg catgttgcgg acacctcttc aggcaggtat cacattgctc gcagaccaca
      721 gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg
      781 cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt tttgcagcgc
      841 agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac gcaggagaag
      901 gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc gcggctggcg
      961 ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga
     1021 accttcggcg cgatattttt ctaaatccga caaacgcacc atataaccgg taacgcgaac
     1081 cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt ccggctttaa aggcaccgag
     1141 gcaaagctgt accagtgcct gcgggttacg tttgatggtt tcgtcgaggg tcagaatgtc
     1201 gctgatgccg gaataataat aagcatgatg cggggcgaca gtttgcagat gggtgatcgg
     1261 atctggctca tcgccatacg gcaaacgcgc gcccggcgtg gtgccgatat cggaactgat
     1321 ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca tatttcacgg gggtattggc
     1381 gacaaactcc gccagttgcg cgctgatgcg ataacccact tcatttgcgg cggcttcttt
     1441 accgtaacgt gcggcaatcc cttctttctc acacagcaag ttaaccgctt ccgccagccc
     1501 atacatgcca aacattggca caaaacgttc agggttaatc agcccttctt tcaccaggaa
     1561 gctattctca aagaagtgtg attgttgata gaggaattca caccgcgcat cgatgatggc
     1621 gatctgctgc tggcagtagt gcggtagagt gcgcgtaaag aagtcatcca gcgattcgct
     1681 gcgctcggca atggctttca ggttaaggcg taccagcgtg ctgccaccac ccgccagcgg
     1741 cagtgagttg taacagctca caatcccgta gccccctttt gtgaaaattt tatcatgcac
     1801 cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg ttcttcgcca cttccagcag
     1861 caggtcatca ggggtgattt caggatcgta gataaaggtc aggttcggtg aaacctgctt
     1921 caactctgcg tctgcacgta agatcgcgcg ggtaatgggc gaatcagacg ggccgatatt
     1981 ggcgtgcata aaggcgtctg gcagggttct gtcgaggtaa cgccagaaac gttttattcg
     2041 aatatcgatc tcgtcttgtg ttagaattct aacatacggt tgcaacaacg catccagttg
     2101 ccccaggtag accggcatcg atgtgaccga cggtacgtgg tggtaaagaa tggtcagcag
     2161 agagagtgcg tcatcaagat ctttcgcgcc ttccagctcc agccattcgg aaccgttcgc
     2221 cagaaaacgg gcgtaatcgg gtaagacata gcgcggtttg tacggcgcat gaccttcaaa
     2281 catatcgcag attacaccct catccagcgc gcggcgggct tcggcaggaa gctgcggata
     2341 aggcagattg ttttctgctt ccagagccag aaaatggcgc ttctgctccg ggctaagcac
     2401 tgggctggtg acaatttgct ggcaacgttg ttgcagtgca ttttcatgag aagtgggcat
     2461 cttcttttcc ttttatgccg aaggtgatgc gccattgtaa gaagtttcgt gatgttcact
     2521 ttgatcctga tgcgtttgcc accactgacg cattcatttg aaagtgaatt atttgaacca
     2581 gatcgcatta cagtgatgca aacttgtaag tagatttcct taattgtgat gtatatcgaa
     2641 gtgtgttgcg gagtagatgt tagaatacta acaaactcgc aaggtgaatt ttattggcga
     2701 caagccagga gaatgaaatg actgatctga aagcaagcag cctgcgtgca ctgaaattga
     2761 tggacctgac caccctgaat gacgacgaca ccgacgagaa agtaattgct ctgtgtcatc
     2821 aggccaaaac tccggtcggc aataccgccg ctatctgtat ctatcctcgc tttatcccga
     2881 ttgctcgcaa aacgctgaaa gagcagggca ccccggaaat ccgtattgct acggtaacta
     2941 acttcccaca cggtaacgac gacatcgaaa tcgcgctggc agaaacccgt gcggcaatcg
     3001 cctacggtgc cgatgaagtt gacgtggtgt tcccgtaccg tgcgctgatg gcgggtaacg
     3061 agcaggttgg ttttgacctg gtgaaagcct gtaaagaggc ttgcgcggca gcgaatgtac
     3121 tgctgaaagt gatcatcgaa accggcgaac tgaaagacga agcgctgatc cgtaaagcgt
     3181 ctgaaatctc catcaaagcg ggtgctgact tcatcaaaac ctctaccggt aaagtggcag
     3241 tgaacgcgac gccggaaagc gcgcgcatca tgatggaagt gattcgtgat atgggcgtag
     3301 aaaaaaccgt tggtttcaaa ccggcgggcg gcgtgcgtac tgcggaagat gcgcagaaat
     3361 atctcgccat cgcagatgaa ctgttcggcg ctgactgggc cgatgcgcgc cactaccgct
     3421 ttggtgcttc cagcctgctg gcaagcctgc tgaaggctct gggccacggc gacggtaaga
     3481 gcgccagcag ctactaagta agatgcttta cgcctgatgc gctgcgctta tcaggcctac
     3541 gagacgtatc tacccgtagg ccggataagg cgtagacgca tccggcaaaa gccgcctcat
     3601 actcttttcc tcgggaggtt accttgtttc tcgcacaaga aattattcgt aaaaaacgtg
     3661 atggtcatgc gctgagcgat gaagaaattc gtttctttat caacggtatt cgcgacaaca
     3721 ctatctccga agggcagatt gccgccctcg cgatgaccat tttcttccac gatatgacaa
     3781 tgcctgagcg tgtctcgctg accatggcga tgcgagattc aggaaccgtt ctcgactgga
     3841 aaagcctgca tctgaatggc ccgattgttg ataaacactc gaccggcggc gtcggcgatg
     3901 tgacttcgct gatgttgggg ccgatggtcg cagcctgcgg cggctatatt ccgatgatct
     3961 ctggtcgcgg cctcggtcat actggcggta cgctcgacaa actggaatcc atccctggct
     4021 tcgacatttt cccggatgac aaccgtttcc gcgaaattat taaagacgtc ggcgtggcga
     4081 ttatcggcca gaccagctca ttggctccgg cagataaacg tttctacgcg acccgcgata
     4141 ttaccgcaac cgtggactcc atcccgctga tcactgcctc gatcctggcg aagaaactgg
     4201 cggaaggtct ggatgcgctg gtgatggacg tgaaagtggg tagcggcgcg tttatgccga
     4261 cctacgaact ctctgaagcc cttgccgaag cgattgttgg cgtggctaac ggcgctggcg
     4321 tgcgcaccac cgcgctgctc accgacatga atcaggtact ggcctccagt gcaggtaacg
     4381 cggttgaagt tcgtgaagcg gtgcagttcc tgacgggtga atatcgtaac ccgcgtctgt
     4441 ttgatgtcac gatggcactg tgcgtggaga tgcttatctc cggcaaactg gcgaaagatg
     4501 acgccgaagc gcgcgcgaaa ttgcaagcag tgctggacaa cggtaaagcg gcagaagtct
     4561 ttggtcgtat ggtagcggca caaaaaggcc caaccgactt cgttgagaac tacgcgaagt
     4621 atctgccgac agcgatgctg acgaaagcag tctatgctga taccgaaggt tttgtcagtg
     4681 aaatggatac ccgcgcgctg gggatggcag tggttgcaat gggcggcggt cgtcgtcagg
     4741 catctgacac cattgattac agcgtcggct ttactgatat ggcgcgtttg ggcgaccagg
     4801 tagacggtca gcgtccgctg gctgttatcc acgcgaaaga cgaaaacagc tggcaggacg
     4861 cggcgaaagc ggtgaaagcg gcaattaaac ttgccgataa agcaccggaa agcacaccaa
     4921 ctgtctatcg ccgtatcagc gaataacggt atactgatct gatcatttaa atttgaagca
     4981 ctgagtacgg agaacatatg aaacgtgcat ttattatggt gctggactca ttcggcatcg
     5041 gcgctacaga agatgcagaa cgctttggtg acgtcggggc tgacaccctg ggtcatatcg
     5101 cagaagcctg tgccaaaggc gaagctgata acggtcgtaa aggcccgctc aatctgccaa
     5161 atctgacccg tctggggctg gcgaaagcac atgaaggttc taccggtttc attccggcag
     5221 gaatggacgg caacgctgaa gttatcggcg cgtacgcatg ggcgcacgaa atgtcatccg
     5281 gtaaagatac cccgtctggt cactgggaaa tcgccggtgt cccggttctg tttgagtggg
     5341 gatacttctc cgatcacgaa aacagcttcc cgcaagagct gctggataaa ctggttgaac
     5401 gcgctaacct gcctggttat ctcggtaact gccactcttc cggtacggtc attctggatc
     5461 agctgggcga agagcacatg aaaaccggca agccgatttt ctatacctcc gctgactccg
     5521 tgttccagat tgcctgccac gaagaaacct ttggcttgga taaactctac gaactgtgcg
     5581 aaatcgcccg tgaagagctg accaacggcg gctacaacat cggtcgtgtt atcgctcgtc
     5641 cgtttattgg cgacaaagcc ggtaacttcc agcgtaccgg taaccgtcac gacctggctg
     5701 ttgagccgcc agcaccgacc gtgctgcaga aactggttga tgaaaaacac ggtcaggtgg
     5761 tttctgtcgg taaaattgca gacatctacg ccaactgcgg tatcaccaag aaagtgaaag
     5821 caactggcct ggacgcgctg tttgacgcca ccatcaaaga gatgaaagaa gcgggtgata
     5881 acaccatcgt cttcaccaac ttcgttgact tcgactcttc ctggggccac cgtcgcgacg
     5941 tcgcaggtta tgcggcgggt ctggaactgt tcgaccgccg tctgccggag ctgatgtctc
     6001 tgctgcgcga tgacgacatc ctgatcctca ctgctgacca cggttgtgat ccgacctgga
     6061 ccggtactga tcacacgcgt gaacacattc cggtactggt ttacggcccg aaagtaaaac
     6121 cgggctcact gggtcaccgt gaaaccttcg cagatatcgg ccagactctg gcaaaatatt
     6181 ttggtacttc tgatatggaa tatggcaaag ccatgttctg ataatgggtg cggcttgatg
     6241 cctcaccccg gccctctccc tcagggagag ggtgaaaaca tgtagataag gataaaacaa
     6301 tggctacccc acacattaat gcagaaatgg gcgatttcgc tgacgtagtt ttgatgccag
     6361 gcgacccgct gcgtgcaaag tatattgctg aaactttcct tgaagatgcc cgtgaagtga
     6421 acaacgttcg cggtatgctg ggcttcaccg gtacttataa aggccgcaaa atttccgtaa
     6481 tgggtcacgg tatgggtatc ccgtcctgct ccatctacac caaagaactg atcaccgatt
     6541 tcggcgtgaa gaaaattatc cgcgtgggtt cctgtggcgc agttctgccg cacgtaaaac
     6601 tgcgcgacgt cgttatcggt atgggtgcct gcaccgattc caaagttaac cgcatccgtt
     6661 ttaaagatca tgactttgcc gctatcgctg actttgacat ggtgcgtaac gcggtagacg
     6721 cggctaaagc actgggcatt gatgctcgcg ttggtaacct gttctccgct gacctgttct
     6781 actctccgga cggcgaaatg ttcgacgtga tggaaaaata cggcatcctc ggcgtggaaa
     6841 tggaagcggc tggtatctac ggcgtcgctg cagaatttgg cgcgaaagcc ctgaccatct
     6901 gtaccgtgtc tgaccacatc cgcactcacg agcagaccac tgccgctgag cgtcagacca
     6961 ccttcaacga catgatcaaa atcgcactgg aatccgttct gctgggcgat aaagagtaat
     7021 tgtgtttcgc tgaaaggcga ttgtcgtgtg aagccggagt gggagactgc tccggctttt
     7081 tagtatctat tcatcaatag caactgattt atagatttac tattattttt catatggata
     7141 gaaaataaaa tctattcaag tatctattca cgaatctatt catttatgtt cagccgtcat
     7201 ccaggaaggg catatcagca actctggtgg acaaccataa atttctgcca ttttgctttt
     7261 gatgtttgag ctaaaccgtt ccgtcttttc gatctatttt agagaattaa tcgagatacc
     7321 taacatttta cttacttcag caagcgataa tttatgcctg atacgcacgg cggctagcca
     7381 ggaaatacgc tggcttaaca ttatattgat aatctcccgc tcctggtttt ccagatcgat
     7441 catattccct tcctttggta ttttaatcag cgagttacct taccgccccc gctatccacg
     7501 tcgacaactc ccgtagctct ttttcctggt ccgggaagtc aaccaacagc gcttcgcact
     7561 cttgttgcag catatccgcg cggtacaggc cgccttgcag tcgcccggcg agggcttcca
     7621 gcggcgcggg gttgaggctg tcggtaaaca cctgggcgcg ggtgatatgg cctttttcaa
     7681 cgtcgaaatg cagttccacg ccgccccagc taaagcgttc atccagcaga tgcgagaatg
     7741 ccggagcctg accgaagttc cattcccagc tactctgacg ggcaaaggtt tcggcgaagt
     7801 ttggcaagtc tggcgttttg tccggggaga tgatttccgc ttctacacgc tcgccataat
     7861 gggcgaaaaa ggccttggtt atggcctcgc aaacctgctc atgggtgatc cccggcagca
     7921 gctcggtgag gttggtcacg cgggaacgca ctgaggtaat gcctttcgcc gccagtttct
     7981 ttttatccgg attgagatag tttgccaggc ggctaaggtc ggcattgagc agcaaggtgc
     8041 cgtggtggaa gccacgatct ttggtttcgc gataggccga tcctgagact ttgcggtcac
     8101 cttcggcggt tttcaccacc agatcgttac gcccggacgc ttcggcgctg acgccaagcg
     8161 cgttcagcgc attgagcaca atcgacgtgg agatggtttt atcgtactcc ggcttgccag
     8221 ccataaaggt aaagcaggta ttgccgagat cgtggaacac cgcgccgcca ccgctactgc
     8281 gccgtgccag gcggacgtta tcttcttcca tccgccgggt attacactct ttccacgggt
     8341 tctgcgcgcg accaatcact accgtgtcgg cattgcgcca gagaaacaga acgcgctgcg
     8401 tggcgggcat ctggcgaaaa atacactcct ccaccgccag gttaaaccac gggccgtaag
     8461 agtcagagat gagcaggcgt aatgtggaca taacgatttc ctttcttgta gtaatgggtc
     8521 actctttttt ctcgctttct tcctcttccg gcaccggttt gctggcggtt aacaggaagg
     8581 gcgattgctg ccagcgggta cgtttaccct gtagcagcgt gcgggtcagc actacgccga
     8641 ttgccagtga aagcagcaac atcaggcgta aaatgttagt ggtgttatcc acctgttggg
     8701 cttcggtggc gagcgtatgg gtgtcgagcg tcaggcgcag atagccgagc ggtccgtttt
     8761 tcccggcaat tggttcgaca atctgctggt taaaatagcc gcccgctttt ttaccgtcga
     8821 gcgccagccg gtcgcgcact tcgacgcttt cgccagaacg tgcgataaga tcgccttgtt
     8881 cgtcatacac acccgcgtcg aggatacggc tttcatccgt taactgatcg aggatcgcct
     8941 gaatgcgttt ttcatccggt gagtcggtac gcatcagcgg tgcaacgttc agcgttacct
     9001 gacgcgccag ggtgcgggcc agttcttcca gctgtggatt acgttgtcgc tggtggtttt
     9061 gactaaacca tgacgctccc tgcatcagcg ccactaacaa ggcaagacag aacaggacaa
     9121 tcactgcccg atgcagccgg aatttcagtt ttgtgcgagc catcttccac cctttgaaaa
     9181 tttgagactt aatgttgcca gaagcaatgg atacaaggta gcctcatgcg ttattttccc
     9241 tgcttcgaac gattttacag gagccttaat gcctaacatt acctggtgcg acctgcctga
     9301 agatgtctct ttatggcctg gtctgcctct ttcattaagt ggtgatgaag tgatgccact
     9361 ggattaccac gcaggtcgta gcggctggct gctgtatggt cgtgggctgg ataaacagcg
     9421 tctgacccaa taccagagca aactgggtgc ggcgatggtg attgttgccg cctggtgcgt
     9481 ggaagattat caggtgattc gtctggcagg ttcactcacc gcacgggcta cgcgcctggc
     9541 ccatgaagcg caactggatg tcgccccgct ggggaaaatc ccgcacctgc gcacgccggg
     9601 tttgctggtg atggacatgg attccaccgc catccagatt gaatgtattg atgaaattgc
     9661 caaactggcc ggaacgggcg agatggtggc ggaagtaacc gaacgggcga tgcgcggcga
     9721 actcgatttt accgccagcc tgcacagccg tgtggcgacg ctgaaaggcg ctgacgccaa
     9781 tattctgcaa caggtgcgtg aaaatctgcc gctgatgcca ggcttaacgc aactggtgct
     9841 caagctggaa acgctgggct ggaaagtggc gattgcctcc ggcggcttta ctttctttgc
     9901 tgaatatctg cgcgacaagc tgcgcctgac cgccgtggta gccaatgaac tggagatcat
     9961 ggacggtaaa tttaccggca atgtgatcgg cgacatcgta gacgcgcagt acaaagcgaa
    10021 aactttgact cgcctcgcgc aggagtatga aatcccgctg gcgcagaccg tggcgattgg
    10081 cgatggagct aatgacctgc cgatgatcaa agcagcaggg ctggggattg cctaccatgc
    10141 caagccaaaa gtgaatgaaa aggcggaagt caccatccgt cacgctgacc tgatgggggt
    10201 attctgcatc ctctctggca gcctgaatca gaagtaattg ctcgcccgcc atcctgcggg
    10261 cggcacagca ttaacgaggt acaccgtggc aaaagctcca aaacgcgcct ttgtttgtaa
    10321 tgaatgcggg gccgattatc cgcgctggca ggggcagtgc agtgcctgtc atgcctggaa
    10381 caccatcacc gaggtgcgtc ttgctgcgtc gccaacggtg gcgcgtaacg agcgtctcag
    10441 cggctatgcc ggtagcgccg gggtggcaaa agtccagaaa ctctccgata tcagccttga
    10501 agagctgccg cgtttttcca ccggatttaa agagttcgac cgcgtactag gcggcggcgt
    10561 ggtgccagga agcgccattc tgattggcgg taacccaggt gcggggaaat ccacgctgct
    10621 actgcaaacg ctgtgcaaac tggcccagca gatgaaaacg ctgtatgtca ctggcgaaga
    10681 gtcgctgcaa caggtggcaa tgcgcgctca tcgccttggc ctgccgactg acaatctcaa
    10741 tatgttgtcg gaaaccagca tcgaacagat ctgcctgatt gccgaagaag agcaaccgaa
    10801 actgatggta attgactcga tccaggtgat gcatatggcg gatgtacaat catcgcctgg
    10861 tagcgtggcg caggtgcgtg aaacggcggc ttatctgacg cgcttcgcca aaacgcgcgg
    10921 tgtggcgatt gtcatggttg ggcacgtaac caaagatggc tcgctggctg gccctaaagt
    10981 gctggaacac tgtatcgact gttcggtgct tctggatggt gatgccgact cccgttttcg
    11041 caccttgcgc agccataaaa accgcttcgg cgcggtgaat gagctgggcg tcttcgcgat
    11101 gaccgaacag gggctgcgtg aagtcagcaa cccttcggca attttcttaa gtcgcggaga
    11161 tgaagtgacc tccggcagct ccgtgatggt ggtgtgggaa ggaacgcgtc cgttgctggt
    11221 ggagattcag gcgctggtcg atcactcgat gatggcgaat ccgcgccgcg tggcagtggg
    11281 gctggaacaa aaccgtctgg caatcctgct ggctgtcttg caccgtcacg gtggtctgca
    11341 aatggccgat caggatgtat ttgtgaacgt ggtcggcggc gtgaaggtaa ccgaaaccag
    11401 tgccgattta gcgttactgc tggcgatggt ttccagcctg cgtgacagac cgctgccaca
    11461 ggatctggtg gtgtttggtg aagtcgggct ggcaggggag atccgcccgg tgcccagcgg
    11521 tcaggaacga atttcagaag cggcgaaaca cggttttcgc cgggcgattg ttccggcggc
    11581 taacgtaccg aaaaaagcgc cggaagggat gcagattttt ggcgttaaaa aactctccga
    11641 cgcgcttagc gtgttcgacg acttataatg agagatacgg agggagatat gtcgtcattt
    11701 gattacctga aaactgccat caagcaacag ggctgcacgc tacagcaggt agctgatgcc
    11761 agcggtatga ccaaagggta tttaagccag ttactgaatg ccaaaattaa aagccccagc
    11821 gcgcaaaagc tggaggcgtt gcaccgtttt ttggggcttg aatttccccg acagaagaaa
    11881 accattggtg tcgtattcgg taagttctac ccgctgcata ccggacatat ctaccttatc
    11941 cagcgcgcct gtagccaggt tgacgagctg catatcatta tgggttttga cgatacccgc
    12001 gaccgcgcgt tgtttgaaga cagcgccatg tcgcagcagc ccacggtgcc ggatcgtctg
    12061 cgctggttat tacaaacttt taaatatcag aaaaatattc gcattcatgc tttcaacgaa
    12121 gagggcatgg agccgtatcc gcacggctgg gatgtgtgga gcaacggcat caaaaagttt
    12181 atggctgaaa aggggattca gccggacctg atctacacct cggaagaagc cgatgcgcca
    12241 cagtatatgg aacatctggg gatcgatacg gtgctggtcg atccgaaacg tacctttatg
    12301 agtatcagcg gtgcgcagat ccgcgaaaac ccgttccgct actgggaata tattcctacg
    12361 gaagtgaagc cgttctttgt acgtaccgtg gcgatccttg gtggcgagtc gagcggtaaa
    12421 tccaccctgg taaacaaact tgccaatatc ttcaatacca ccagtgcgtg ggaatatggt
    12481 cgcgattatg tcttttcaca cctcggcggt gatgagatcg cattgcagta ttccgattac
    12541 gataaaatcg cgctgggcca cgcacaatac attgattttg cagtgaaata tgccaataaa
    12601 gtggcgttta ttgacactga ttttgtcact acccaggcgt tctgcaaaaa gtacgaaggg
    12661 cgtgagcatc cgttcgtaca ggcgttgatt gatgaatacc gtttcgatct ggtgatcctg
    12721 ctggagaaca acacgccgtg ggtggcggat ggtttacgca gcctcggcag ttcggtggat
    12781 cgcaaagagt tccagaactt gctggtggag atgctggaag agaacaatat cgaattcgtg
    12841 cgggttgaag aggacgatta tgacagccgt ttcctgcgct gcgtggagct ggtgcgggag
    12901 atgatggggg agcagagata accgcgatga aacggctcaa aggcgaggta taaaataagt
    12961 tttttgatga gttgaaaacc tctctggaag aggctgtcga gattaaacaa ggtttaaaag
    13021 aatcagcacg ggtgacccgc tacgaatttg cggatactaa ggctgttgta ggccggaaaa
    13081 ggcgttcacg ccgtatccgg cattcggtgc tcaatgcctg atgcgacgct taccgcgtct
    13141 tatcaggcct ggagattcac aacgtattga aatttcttgg tttgtaggcc ggataaggcg
    13201 ttcacgccgc atccggcatt ttacgcatta cttcgcaata cgcttgtact tgatacgctt
    13261 cggctccagc gcgtctgcgc ccagcgtgcg ttttttgtac tcttcgtatt cggtaaagtt
    13321 accttcgaag aactcaactt taccttcatc ctggtagtcc aggatgtgcg tggcgatacg
    13381 gtcgaggaac caacggtcgt gcgagataac catcgcacag cccgggaact ccagcagggc
    13441 gttttccagc gcgcgcaggg tttcgatatc caggtcgttg gttggttcgt cgagcagcag
    13501 catgttgccg ccaacctgca gcagcttcgc cagatgcaga cgaccgcgct caccaccgga
    13561 aagttcacca acgcgtttac cctgatcaac ccctttaaag ttaaagcggc caacgtaggc
    13621 gcggcttggc atctcggtgt tgccgatctt cataatatcc agcccgccgg aaacttcttc
    13681 ccaaacggtt ttgctgttat ccattgagtc acggaactga tcaaccgatg ccagtttcac
    13741 cgtttcaccc aaagtgatgg tgccgctgtc cggctgttcc tgaccggaga tcatacggaa
    13801 cagggtcgat ttacccgcgc cgttcggacc gatgatcccg acgatcgccc ctttcgggat
    13861 cgagaagctc aggtcatcaa tcagcaggcg atcgccgtag gatttacgca ggttgctgac
    13921 ttccagcact ttatcgccca gacgcggtcc aggtggaata aacagttcgt tggtttcgtt
    13981 acgtttctgg tattcggtgc tgttcagctc ttcaaagcgt gccagacgtg ctttaccttt
    14041 cgactgacgg cctttagttc cctgacgtac ccactccagc tctttctcga tcgacttacg
    14101 acgcgccgct tcttgtgaag cttcctgcgc cagacgctga tctttctgct ccagccagga
    14161 ggagtagtta ccttcccacg gaataccttc accgcggtcc agttcgagga tccagcctgc
    14221 aacgttatcg aggaagtaac ggtcgtgagt aatcgccacc acggtgcctt cgaagtcgtg
    14281 caggaagcgt tccagccagg ccacggattc ggcatccagg tggttggtcg gttcgtcgag
    14341 cagcagcatg tctggttttt ccagcagcag gcggcacaac gccacgcgac gacgctcacc
    14401 accggagagg ttagcgattt tcgcgtccca gtccggcaga cgtagcgcat ccgccgcacg
    14461 ctccagctgt acgttcagat tatgaccgtc gtgagcctga ataatctctt ccagacggcc
    14521 ttgttcagcg gccagcttgt caaaatcggc atccggatcg gcgtacagcg catacacttc
    14581 atccaggcgt ttcagggcgt taaccacttc agaaaccgct tcttcaatag actcacgcac
    14641 ggtgtgttcc gggttcagct gcggttcctg cggcagataa ccaatcttga tgtctggctg
    14701 cggacgcgct tcaccttcga tgtctttatc aatgcccgcc ataatgcgca gcagggtgga
    14761 cttacccgcg ccgttcagac ccaggacacc aatttttgcc ccagggaaga aactcagaga
    14821 gatgtttttc aaaatatgac gtttcggcgg aacaactttg ccgacacgat gcatggtata
    14881 aacgaattga gccacgttgg acttcgcctc tatgtttatc gtgatgatga gttttcaaag
    14941 gcgaagtgta gcctttttcc ccgcctaatc ccagttggtc gatcaccttc gcagtaaaag
    15001 taaaaaagtg tccgtaacgt ggcgtaaacg gcaatgactg gttagcataa atctattacg
    15061 cggcatgacg ctgcattgat gtatttacac ttagaggatg cgcttgtgga aaaagccaaa
    15121 caagttacct ggcggctgtt ggctgccggt gtctgtctgc tgacggtcag cagcgtggcg
    15181 cgagccgact cactggatga gcagcgtagt cgttacgcgc aaatcaagca ggcctgggat
    15241 aatcgacaaa tggatgtggt cgaacaaatg atgcctggac tgaaggatta tccgctttat
    15301 ccctacctgg aataccgcca gatcaccgat gacctgatga atcaaccggc ggtgacggtc
    15361 actaactttg ttcgcgctaa ccccacgctt cctcccgctc gcacgctgca atctcgtttc
    15421 gtcaatgaac tggcgcggcg tgaagactgg cgtggcttgt tagcctttag cccggaaaag
    15481 cccggaacta ccgaagcgca atgtaattac tactatgcga aatggaacac cgggcagagt
    15541 gaagaagcct ggcaaggggc gaaagagctg tggctaaccg gcaagagcca gcctaacgcc
    15601 tgtgacaagt tatttagcgt ctggcgtgcg tcaggtaaac aagatccgct ggcgtattta
    15661 gagcgtatcc gcctggcgat gaaagcgggt aacactggcc tggtaacagt gctggcaggg
    15721 cagatgcctg ccgattacca gactatcgcc tcggcaatca tttcactggc gaacaaccct
    15781 aatacggtac tgaccttcgc gcgtacaacc ggcgcgaccg attttacccg tcaaatggcg
    15841 gcggtggcgt ttgccagtgt ggcgcggcag gatgctgaaa atgcacggct gatgatcccg
    15901 tcgcttgccc aggcgcagca gcttaatgaa gatcagattc aggagctgcg cgatatcgtc
    15961 gcctggcgtt tgatgggcaa cgatgtcacc gacgagcagg cgaaatggcg cgatgacgcc
    16021 attatgcgct cgcaatccac gtcgttgatt gaacgccgtg tgcgaatggc gcttggcacc
    16081 ggcgatcgtc gcggcctgaa tacctggctg gcgcgtctgc cgatggaagc aaaagagaaa
    16141 gatgaatggc gttactggca ggcggattta ttgctggaac gcggacgtga agctgaagcg
    16201 aaagagattt tgcatcaact catgcaacag cgtggtttct acccgatggt tgctgcacaa
    16261 cgcatcggcg aagagtatga gctgaagatt gataaagcgc cgcagaatgt tgacagcgcc
    16321 ctgactcagg ggccggagat ggcgcgcgtg cgcgagttga tgtactggaa tctcgataac
    16381 accgcgcgta gcgagtgggc caatctggtg aagagcaagt caaaaacaga gcaggctcaa
    16441 ctggcgcggt atgctttcaa caaccaatgg tgggatctta gcgttcaggc aacgatcgcc
    16501 gggaagctgt gggatcatct ggaagagcga ttcccgctgg cttacaacga tcttttcaaa
    16561 cgctacacca gcggtaagga gatcccgcaa agctatgcga tggcgattgc ccgtcaggag
    16621 agcgcctgga atccgaaagt gaaatcaccg gtaggggcca gcggcctgat gcagattatg
    16681 cctggtacag cgacccatac ggtgaagatg ttctctattc ctggttatag cagccctggg
    16741 caattgctgg atccggaaac gaacatcaac attggcacca gttacctgca atatgtttat
    16801 cagcagtttg gcaataaccg tattttctcc tcagcagctt ataacgccgg acctggacgg
    16861 gtgcgaacct ggcttggcaa cagcgccggg cgtatcgacg ctgtggcatt tgtcgagagt
    16921 attccgttct ccgagacgcg cggttatgtg aagaacgtgc tggcttatga cgcttactac
    16981 cgctatttca tgggggataa accgacgttg atgagcgcca cggaatgggg acgtcgttac
    17041 tgatccgcac gtttatgata tgctatcgta ctctttagcg agtacaaccg ggggaggcat
    17101 tttgcttccc ccgctaacaa tggcgacata ttatggccca acaatcaccc tattcagcag
    17161 cgatggcaga acagcgtcac caggagtggt tacgttttgt cgacctgctt aagaatgcct
    17221 accaaaacga tctccattta ccgttgttaa acctgatgct gacgccagat gagcgcgaag
    17281 cgttggggac tcgcgtgcgt attgtcgaag agctgttgcg cggcgaaatg agccagcgtg
    17341 agttaaaaaa tgaactcggc gcgggcatcg cgacgattac gcgtggatct aacagcctga
    17401 aagccgcgcc cgttgagctg cgccagtggc tggaagaggt gttgctgaaa agcgattga
//
LOCUS       DAHBHC010000101        17015 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHBHC010000101 DAHBHC010000000
VERSION     DAHBHC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.102"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            62..493
                     /gene="tnpA"
                     /locus_tag="M2A35_004236"
     CDS             62..493
                     /gene="tnpA"
                     /locus_tag="M2A35_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCB7200459.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(509..697)
                     /locus_tag="M2A35_004237"
     CDS             complement(509..697)
                     /locus_tag="M2A35_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCB7200460.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="M2A35_004238"
     CDS             complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="M2A35_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCB7200461.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1231..1869)
                     /gene="leuE"
                     /locus_tag="M2A35_004239"
     CDS             complement(1231..1869)
                     /gene="leuE"
                     /locus_tag="M2A35_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCB7200462.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1996..2919)
                     /gene="dmlR"
                     /locus_tag="M2A35_004240"
     CDS             complement(1996..2919)
                     /gene="dmlR"
                     /locus_tag="M2A35_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCB7200463.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3022..4107
                     /gene="dmlA"
                     /locus_tag="M2A35_004241"
     CDS             3022..4107
                     /gene="dmlA"
                     /locus_tag="M2A35_004241"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCB7200464.1"
                     /translation="MMKTMRIAAIPGDGIDKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4358..5968
                     /gene="yeaV"
                     /locus_tag="M2A35_004242"
     CDS             4358..5968
                     /gene="yeaV"
                     /locus_tag="M2A35_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCB7200465.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFLTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6000..7124
                     /gene="yeaW"
                     /locus_tag="M2A35_004243"
     CDS             6000..7124
                     /gene="yeaW"
                     /locus_tag="M2A35_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCB7200466.1"
                     /translation="MSNLSPDFVLPENYCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7180..8145
                     /gene="yeaX"
                     /locus_tag="M2A35_004244"
     CDS             7180..8145
                     /gene="yeaX"
                     /locus_tag="M2A35_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCB7200467.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8199..9314)
                     /gene="rnd"
                     /locus_tag="M2A35_004245"
     CDS             complement(8199..9314)
                     /gene="rnd"
                     /locus_tag="M2A35_004245"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCB7200468.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9396..11081)
                     /gene="fadD"
                     /locus_tag="M2A35_004246"
     CDS             complement(9396..11081)
                     /gene="fadD"
                     /locus_tag="M2A35_004246"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCB7200469.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11286..11867)
                     /gene="yeaY"
                     /locus_tag="M2A35_004247"
     CDS             complement(11286..11867)
                     /gene="yeaY"
                     /locus_tag="M2A35_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCB7200470.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11907..12602)
                     /gene="tsaB"
                     /locus_tag="M2A35_004248"
     CDS             complement(11907..12602)
                     /gene="tsaB"
                     /locus_tag="M2A35_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCB7200471.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12660..14570)
                     /locus_tag="M2A35_004249"
     CDS             complement(12660..14570)
                     /locus_tag="M2A35_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCB7200472.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14763..15740
                     /locus_tag="M2A35_004250"
     CDS             14763..15740
                     /locus_tag="M2A35_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCB7200473.1"
                     /translation="MSDSVTVGIDVSKLTLDVHVSPAQLRQTLSNSDAGFEELKTLLS
                     MQQVSLVLLESTGGYEDAVVCFLQSLGYDVSVINPRQARDFARSMGRLAKTDRIDAEL
                     LAQLASVIDSRPDRARFVKKITDDRRRKLACMVTRRRQVVNMISTEQQRKSAASVYST
                     ESINRVLSFLRSELKSIDADISLHVRSHFADISGLLASFKGIGPATIGVLLGEVPELG
                     RLNRRQISALIGVAPFNRDSGIMRGRRTVWGGRTMVRNALYMPTLSAVRFNPVIREFY
                     TRLISQGKPRKLAQVAAMRKLLCIINAMLSSGTMFNPALHLHSVQEAQQ"
     gene            15737..>17015
                     /locus_tag="M2A35_004251"
     CDS             15737..>17015
                     /locus_tag="M2A35_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7200474.1"
                     /translation="MKTESAILTAYNAIDAASVALGRPCLTLPEREHFQAERDVATRN
                     LATLSRQYRDEVGALSSQLALPRNTQVDRVIYGINCTDLSLADRGRAAGLVSQFGSFS
                     FTVAHAFCRLVYQLGVKNSLMALESAHRFAFFNQDGDPYEFSLFCTDEQLAEVADSVV
                     RDCLVSRAIYSDVPEQHLLNVMVYFQAISGFDFAPVYATYHERYGDEGLLKRLTDLAF
                     VTRFLRVVRDQRVNEVCRMLGILNRTTPYISDWHRDLFAVRSKRVKKYLRSSGVFDAF
                     NELVCTLEDAHNASVSNPKNRIAELCVRGKAVCELSEDMGLSGYFIVLTTPSRFHPTT
                     SFKVAGKWHSRPNKKWWEAGCPTIKDSHAWLNTVWRRVCRRLDKAGIQIPGLRTVEPH
                     ADGTTHWNFLIYCNPHESATVLAIFREEAMRDEP"
BASE COUNT         4014 a         4516 c         4212 g         4273 t
ORIGIN      
        1 ctcctgttga tcacctggtc ttaactggaa tttaaatgcc tgtagtcgtt tcacaggcca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac cagatacaga cgtcaaattt ttgaccatga
      181 cgcgacagaa aaactacgcg cttacttttc aaatgtatgt gctgattttg aagctgaact
      241 tgttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc ctcccaaact
      301 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga
      361 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt
      421 tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
      481 aacacctggt taggtggaaa accgcgcctt atatccccgc cctgaaggac ggggttttac
      541 ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca gccgccactt
      601 ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg ccattgctgt
      661 tattggtgac ggtcacggta tacgttgctt tgcccatgat tcatttcccg ttatgaatga
      721 ctttccgttg ttgcgcacct tccatcagga cttcaggagc cacgaagaag tcaatgttga
      781 aataagtatc gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg aacaccgcaa
      841 actgccccgc ttcgataagg atcacctgat caggctctgc actgtgttca tcagcgtagc
      901 cgagatattt gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc
      961 ctttatcaag atgacgttcg aatattccgg caggcgcagt ttgtttattc cagaaaggcg
     1021 ttgagcgcgt atgaatataa ttctgtggga tttgcagcat ctttttccct ccttcgttga
     1081 atgcgctgaa aacggtattc cagccgtttc agagtacgcc tgataatttg cattttaaat
     1141 accatttatt ggttattttt tagcatcata tcagagaaga atcagggagg attacaagtg
     1201 ggaagcccgc gacaacgcgg gctgaatgta tcaggattgc agcgtcgcca gtcgggcagc
     1261 gaaacccacg aacatcaaac caatcagtga gttgcccact ttagtcagtt tctttttggt
     1321 acgtatgtac tgcgtgacaa aagcaccaga aataatcagg aagctcaaat agcagaaact
     1381 caccagttcc agcgtcgtcg ccagaataaa gaatgaaatt cccgtatgtg gggcattaac
     1441 atcgataaac tgtacgaaaa acgacacata gaacaaaatg gctttcggat tagtcaggct
     1501 caaaattaac gcgcgtttaa aaatagcacc gtattggggc tcatcggatt tggcctcgtt
     1561 atttttaccc ttcagggtcg cgtagagaat tttactcccc agatagagca aataaaacgc
     1621 accaagataa cgtataatgt tgaataatat cggggtggtc ttaattaatg tcgccactcc
     1681 agcccatgcc agaaacatca ataccgcatc gccaataaat acaccgcagg ccgcaagata
     1741 accgcctttc ataccgctac tgacgctatt tttgagtaca aacagggtat ttggccctgg
     1801 caccaacaca ataaaaatgg ccccaaccag ataggtccag taattcagaa ccccgtattc
     1861 agcgaacaca ttaacctctt taattatctt tcgatcatgc gcgattaaag gtgaatatgc
     1921 taaccaatct gtagcggctt agaaaggaga aaatcaggtt ttaacccgat atcaacccga
     1981 taattgaata attaatcagg catgctccag tgaaaaattc gggtagtgct cggcaaaata
     2041 ctggcgtaaa aattctaccg ttatccgcac tttcgctgac gtcgccagcc ttgaaacata
     2101 aacggcccag acgttcgctg gctggtaata ttccggtagc acttgcacta aatgaccact
     2161 ggcaatgttt tcgctaacat cccaccagga acgcagagca atcccctgcc catcgagaca
     2221 ccactgatgg acaatttctc catgattaga cgacagtggg ccggtgactt taatcgcatg
     2281 gtggccttct ttattgcgta attgccagac accaaaagga tgatcgcgct ctttaatgac
     2341 caggcacggc aatgcagaaa gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa
     2401 ttccggcgac gcgcagagaa tgcggtaatt ggtcgccagt ttgcgggcga ttaaattagg
     2461 ggcgatgtca tcgccaatgc gaatatcgag atcgacacct tcattgacca aatcgaccag
     2521 tcggtcttcc acatcaaaac gtaattccag ttgcggatac gccttcgcca gcgctgataa
     2581 cgccggagcc accacctgtc gtccaaaacc aaagctgctg ataatacgca gcatcccctg
     2641 gggcacctga cgcacgtcag aaagttcgtc catcatctga ccgacatcct gcaaaatccg
     2701 ctgcgcccat tcataaatcc gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg
     2761 gtgtaacaac accacgttta gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc
     2821 cggtgaaacg cccagttctt ccgccacggc ggcaaaacca gcccgacgag ccaccagcat
     2881 aaagacgcgc aaatcattca gcagcggtaa attattcatg attcgtgttt tatgtttcac
     2941 cagttacggg gattaattcc ttttcagtca attataggat ggtgatgttg tcaattttga
     3001 tggtcaggaa gtgagaaccc aatgatgaaa acgatgcgta ttgctgcgat cccgggagac
     3061 gggattgaca aagaagtcct tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc
     3121 tggggcttcg acttgagttt tgagcaaatg gagtgggcga gctgcgagta ttacagccat
     3181 cacggtaaaa tgatgccgga cgactggcat gagcaactta gccgtttcga cgccatctat
     3241 tttggtgccg tcggctggcc ggataccgtt ccggaccata tttcgttgtg gggttcgctg
     3301 ctgaaatttc gtcgtgaatt cgaccagtac gtcaacctgc gcccggttcg tctctttcct
     3361 ggcgttccct gcccgctggc gggaaaacag cctggcgaca tcgattttta cgtggtcagg
     3421 gaaaacaccg aaggcgaata ttcctcgctc ggcggtagag tgaatgaagg tacagagcat
     3481 gaagtcgtca ttcaggaatc ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat
     3541 gccttcgaac ttgcgcaaag ccgcccacgt aagacgctaa cttctgccac taaatcaaac
     3601 ggtttagcca tcagcatgcc gtactgggat gagcgagtgg aagcaatggc cgagaattac
     3661 ccggagatcc gctgggacaa gcagcatatt gatattctct gcgcgcgttt tgtgatgcag
     3721 ccggaacgct tcgatgtggt ggtggcgtcc aatttatttg gcgatatcct ttccgatctt
     3781 ggcccggcct gcaccggcac cattggcatt gccccatccg ccaacctgaa tccggaacgc
     3841 actttcccgt cgctcttcga gccagtccac ggttccgcgc cggatatcta cgggaaaaac
     3901 attgctaacc ctatcgccac gatttgggcc ggtgcaatga tgctcgattt tctcggcaat
     3961 ggcgatgagc gtttccagca agcgcataac ggtattctgg cagcaattga agaagtgatt
     4021 gctcacgggc cgaaaacacc tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac
     4081 gcgatttgca aaattatttt gcgttaaggt caaaccagtt tatttgaacc gcgtcactga
     4141 cgcggttttt ttattcgttc tttgcagtaa ataacctgcg tcatttcacc ttttattgtt
     4201 tccgtttcgt gttttatggc tttccgtatt cttaattgtt taatttatgt aacatgcaaa
     4261 taatcaattt gttaaacaca acatacaact cattaccctg caaataatta caactggcaa
     4321 aagccagaga cgggctttta tgcccaggag atgactcatg atgagcaatg ttaagaaaaa
     4381 agatgtgccg ctgataagca tcagcctggt ggccattctt ttcatcgcag ctgcattaag
     4441 ccttttccca caacaatcgg ccgacgcggc caacgcaata tacacttttg ttacgcgtac
     4501 gttaggttcc gccgtacagg tattggtttt gctggcaatg ggactggtga tttatttagc
     4561 caccagtaaa tacggcaata ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc
     4621 ctggctgttt atgtttattt gtgccggttt aggttcttct acgctttatt ggggggttgc
     4681 tgaatgggcc tattattatc aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc
     4741 actcgaattt agcgtccctt actccttctt ccactggggc atcagcgcct gggcaactta
     4801 tacgctggcc tcattaatca tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag
     4861 tctttccggc attattgccg cgattaccgg cgttcgcccg caaggcccat ggggaaaact
     4921 ggttgatttg atgttcctga tcgccactgt cggcgcactg actatttccc ttgttgtcac
     4981 cgcagcaacc tttactcgtg gactttccgc gctgaccggt ttacccgata acttcaccgt
     5041 gcaggcattt gtgatcctgc tttccggcgg cattttttgc ctaagctcgt ggattggtat
     5101 caacaacggt ttgcaacgtc tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt
     5161 actggtgctg attgtcggcc caaccgaatt tattaccaac agcatcatca atgccatcgg
     5221 tctgaccacg caaaacttcc tgcaaatgag cttattcacc gatccgcttg gcgatggttc
     5281 atttacccgc aactggaccg ttttctactg gctgtggtgg atctcataca cccctggcgt
     5341 agcaatgttt ctcacccgcg tttcccgcgg tcgtaagatt aaagaagtta tctggggact
     5401 gatcctcggc agcaccgtcg gttgctggtt cttcttcggc gtaatggaaa gctatgccat
     5461 tcatcagttt atcaatggcg taatcaacgt cccacaggtg ctggaaacac tgggcggcga
     5521 aacagctgta cagcaagttc tgatgtcgtt gccagccggt aaattgttcc tcgccgcata
     5581 cctaggcgtg atgattattt tccttgcctc gcatatggat gcggtggcct acaccatggc
     5641 tgcgaccagt acgcgtaatc tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt
     5701 ctggtgcgtg gtgatcactc tgatcccgct ttccatcttg tttaccggtg cttcgctgga
     5761 aacgatgaaa accaccgtcg tgctcacagc ccttcccttc ctcgtcattt tactggtgaa
     5821 agtcggcgga tttattcgct ggctgaaaca ggattacgcc gacattccgg ctcatcaagt
     5881 tgaacattat ctcccgcaga caccggttga agccctggaa aaaacgccag tgctccctgc
     5941 gggaaccgta ttcaaaggcg acaactgagc gccatcatcc taacgataaa ggtatcccta
     6001 tgagcaatct gagccctgac tttgtactac ccgaaaatta ttgcgctaac ccgcaagagg
     6061 cgtggaccat tcctgcccgt ttttataccg atcagaacgc gtttgaacac gaaaaagaga
     6121 acgtcttcgc caaaagctgg atttgcgtcg ctcacagcag cgaactggcg aatgccaatg
     6181 attatgtgac gcgtgagatc attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg
     6241 ttttgcgcgc gttctataac gtgtgtccgc accgtggtca tcagttgttg agcggtgaag
     6301 gaaaagcaaa aaatgtgatt acctgcccgt atcacgcatg ggcattcaaa ctcgatggca
     6361 acctggccca tgcacgtaac tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac
     6421 tggttccggt gcgtctggaa gaatatgccg gattcgtctt catcaacatg gaccccaacg
     6481 ccaccagcgt agaagatcaa ttacccggcc tgggcgcgaa agtgctggaa gcctgcccgg
     6541 aagtccacga tctgaaactg gcggcccgct ttaccacccg cacgcctgcc aactggaaga
     6601 acattgtcga taactatctc gagtgctatc actgtggtcc ggcgcatcca ggtttctccg
     6661 actccgtaca ggttgatcgt tactggcaca ccatgcacgg taactggacg ctgcaatacg
     6721 gtttcgccaa accgtccgaa cagtcgttta aatttgaaga gggtacggat gcggcattcc
     6781 acggtttctg gctgtggccg tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga
     6841 cggtcattta tgaattcccg gtggattctg aaactaccct gcaaaactac gatatttact
     6901 tcaccaatga agagttaacc gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt
     6961 tccgtccgga agatttacgt ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct
     7021 atcgtggtca ggggcgcatc atggccgaca gtagcggtag tggcatttcc gaacatggta
     7081 tcgcccattt ccataatctg ctggcgcagg tgtttaagga ctaatgacat cggcggcggt
     7141 attttccgcc gctgggctga tttttgatgg agtacagcaa tgtcagacta tcaaatgttt
     7201 gaagtacagg tgagtcaggt ggaaccgctt accgaacagg tgaaacgctt cacgctggtg
     7261 gcaaccgatg gcaaaccgct gcctgcgttc accggaggaa gtcacatcat tgtgcagatg
     7321 agcgatggtg ataaccagta cagcaatgcg tattcactac tgagttcgcc gcatgacacc
     7381 tcttgttatc agattgccgt tcggctggag gaaaactcgc gcggcggttc ccgctttttg
     7441 catcagcagg taaaagtggg cgatcggtta acgatttcaa cgcctaataa cctgtttgcg
     7501 ctaattccct cagccagaaa gcatctgttt atcgcgggcg gtattggtat cacccctttc
     7561 ctgtcgcaca tggcagagct gcaacacagc gacgtcgact ggcagctaca ttactgctcg
     7621 cgaaatccag aaagttgcgc atttcgtgat gagctagtcc agcatccgca ggctgagaaa
     7681 gttcacttgc atcactcatc aaccggaaca cgactggaat tagcgcgatt attggcggat
     7741 atcgaacctg gcacacacgt ttatacctgt ggtcccgagg cgctaaatga agcggtaaga
     7801 agtgaagctg cgcgtctgga catcgtcgcc gatacgctgc actttgagca atttgctatc
     7861 gaagacaaaa ccggcgatgc ctttaccctg gtgcttgccc gttccggaaa agagtttgtg
     7921 gtgccggaag agatgactat tttgcaggtt attgaaaata ataaagccgc gaaagtggaa
     7981 tgtttatgtc gtgaaggggt atgcggaacc tgcgaaacag caatactgga aggtgaagct
     8041 gaccatcggg atcagtattt tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc
     8101 tgttgttcgc gtgcgaaggg taaacgcctg gtgttggatt tgtagtttgc caaagccgga
     8161 tgtggcgctg agcgcgccca gtccggcttc ggaagatttt actgcggata ttcctgcaat
     8221 aaattgtgta atgcttccgc catcagctca ccacgccagc cggaaatcag ctccggcaaa
     8281 ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat
     8341 gccagcaatt cggcgctgat cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc
     8401 gctttaaacg ctttacgata accaggcatg tccatcaggt taagcatcgg ctgcggtaag
     8461 gcctcttccg gcaatgtctg cgctttttcc accagcgcca gcagcgtttt accgtgaaag
     8521 cggatttcgc taccggataa acccaggctg tccagttcgc ctaaacttcc cggcatataa
     8581 cgcgcgaccg accacaaatg ctcttcacgc acgacaaagt tcaccgccag atcacgctct
     8641 cgcgccttgc gcagtcgcca gtcggctaac agttgcagac aggccagttg gcgtgtgcgt
     8701 aactgccagg cattggtgat atcacgccag gcatcttccg gcgcaacgac ttcctgacga
     8761 cgcatttgca tcaggcggca ttcatccagc gccgcaggta gccagccgga ggcctccgtt
     8821 tctaccataa gcttggcggt gatcggtaac agataccaga catccgccgc tgcgtattca
     8881 cactgacgtt cggtcagcgg tctggccagc cagtcggtgc gcgattcact cttgtccagc
     8941 gtaacgccgg aatactcttc caccatggaa gcgaaacccc atgacatcgg gcgtccgcag
     9001 aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa gacattgagg
     9061 aacacttcca gatcttcact gcctgcatgg agaaattttg tgatggacgg atcgcgcagg
     9121 atcgctttca gcggtgacca gtcggtgatc ccgagaggat caattagcgc cagatgctcg
     9181 ccatcgaaaa gttgaatcaa ccccagctgc gggtaataag tgcgcgtacg aacaaattca
     9241 gtatccaggg ctatcgccgg aaaggcacgg acggcttcac acaaagaagc cagcgcatcg
     9301 tccgtggtaa tcatttggta attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg
     9361 ccggattaac cggcgtctga cgactgactt aacactcagg ctttattgtc cactttgccg
     9421 cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga cgttagattt cggtaactca
     9481 tcacgaaact ccaccagctt cggtactttg tagcccgtga gctgacggcg gcaaaaggtc
     9541 accagtgact cttcggtaag cgatggatct tttttcacta cgaagatttt caccgcttca
     9601 ccactggagc cggaaggtac gccaacagcc gcgacttcct gtacgccagg atgctgcatg
     9661 acgacatctt caatctcgtt gggatagacg ttaaaaccgg aaaccagaat catgtctttt
     9721 ttacgatcga caatgcgcag gaacccttct tcatccatca ccgcgatgtc gccggtgtgt
     9781 aaccagccat ttttgatgat ctcatctgta gcatccggac gctgccagta acccagcatc
     9841 acctgcggtc ctttgacaca aagctcaccc ggttgacccg gtggtacttc attatcatca
     9901 tcatccacca gtttggcttc cgtcgacggc accggcaaac cgatgctacc actatgataa
     9961 tcaatatcat atgggttaac gctgaccagc ggcgcacact cggtaaggcc atagccttcc
    10021 agcagatact gtcctgtcag tttcacccaa cgctctgcca ccacttgctg cactggcatc
    10081 cctccgcctg cggaaagatg cagactggag aaatccagct gctggaactc tttattgttc
    10141 agcaacgcat tgaacaaggt gttaacgccc gtgatagcgg taaacggata tttcgctaac
    10201 tcttttacca accctggaat atcgcgcggg ttagtgataa gcaggttctg cccacccagt
    10261 tcgataaaca gcaggcagtt aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc
    10321 accaccagct ctttgcccgg atgcaacagc ggaccatagg tcgcgttaac ctgttccagg
    10381 ttcgccagca tattgcggtg agtcagcatc gcgcctttcg ccacaccagt ggtgccgccg
    10441 gtgtattgca gaaaagctaa atcttccggc accagttcgg gtttgacgta ctgcatccgg
    10501 tagccgttat gcagtgcgct acgaaatgaa atggcatctg gcagatggta tttcggcacc
    10561 aaacgcttga tgtatttaac aacgaaattg actaccgtgc cttttgccgt agatagctga
    10621 tcgcccatac gggtcagaat tacgtgctga acggcggttt tatcaaccac tttttccagt
    10681 gtgtgagcaa agttagacac gataacaatc gccgatgcgc cgctatcgtt aagctgatgc
    10741 tcaagctcac gcggggtata caacgggtta acgtttacga cgatcatccc tgcacgcaaa
    10801 atgccaaaca gcgccaccgg atattgcaat aaattaggca tcatcaacgc aacgcgatcg
    10861 cctttcttca gccccaaccc ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc
    10921 agcttgcgga aggtcattac ctcccccata ttcacaaacg caggctgatc ggcgtagcgc
    10981 gcgaccgact gctcaaacat atctaccaga gattgataac ggtcagggtt gatctccgtc
    11041 ggaacgtccg cgggataacg gttaagccaa accttcttca attcttcacc tctaaaatgc
    11101 gtgttcgtcg tcatcgcaac cccaaatgat atacatgccg ttaacataat attaactcat
    11161 cataccagct tgataattac ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta
    11221 tctttaccgt aagaatgcag aaacagcgga ccagccgctg tttctttttc tttaaaaaca
    11281 agcaattatt cagttacaac tgtttgtact ctcgcgggac cgggattata ccagccccat
    11341 ccgccgtagc catagggcca gccacgaccg ccataaaacc atggatcaat cggctgaggc
    11401 ggcataatca cctgctgggt taaatgccaa cgtttgtaac ccgttacttg catcaccata
    11461 aatttatagg gcgtattgcc gattttgccg tcaaccgcac cagtgattgg cccaactacc
    11521 gtaaccagtt gtccacggaa atccaccggg tccagaaaac cgttcacatc ggcataaatg
    11581 cgaccgcgag aaggttctcc cagcgtcggt ctggctccgc tgtccagcgg tacggtagca
    11641 atttccagac gggttttccc ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt
    11701 gcctcctgac caacgtacag ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc
    11761 gtgggactgc tgcctttaat ggcgtccggc acagtgacac aaccgctcag cattagcgca
    11821 aacgtacctg ccagtatgcc tttgataaca ttcttttgaa ccgccatgat gcgactcctt
    11881 tttttctcag ggcatactct taagattcat tctttgcccg gaagtttctt ccatgcgacg
    11941 ttgttacgta aataaaccgg ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac
    12001 atctgacacg caatcggcag catatcttcc gccgctggca gtaaaacttc tccgtcgcgc
    12061 aaaaccagcc cgctctcttt accgagatcc ggccaggctt gccagcccgt ccctaccgtt
    12121 acccattcgc cggaaagctg ttgcattcgt tcatgaacga gttcgggttt gagtacggct
    12181 tcggtttctt caccgtgcca gataccgttt tcatcacgct gatattcggc ccagtaaact
    12241 tcgcccattc gcgcgtcaat ggctgccagc acgcgggttg cgccgttttt gcgccacgcc
    12301 ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc
    12361 agcccttgcg caatgccaat accaatgcgc acgccggtaa agctaccggg gccgcgcccg
    12421 taagccagag cgttaatatc agtcagggaa gttccgctgg tggtcaggat atcctgcacc
    12481 atcggtaaga ttcgttgagt atgttcacga gggcaaagct caaaatgagc gttgacagta
    12541 ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc
    12601 atgggtcttc gtgcttagat caataaaaag gcgcgcatca taccatactc cgtaacaaat
    12661 tacctggagg atggtatcgc aaggaaacga accgcacggg caatgtcacg agtgcgtggc
    12721 gcgggcggca gactggcgag aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca
    12781 caaatcacca gcacgccacg atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg
    12841 agagtaatga cggcatctgg tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga
    12901 caatcttcca tgcgcgcttt taacagtgga tcatccggcg aggtaaacgg caacttgtcg
    12961 ataatcacca atgacaatgt atcgccacgc acgtccaccc cttcccagaa actactggtt
    13021 gccacaagaa gcgcgttacc ggcgctgaca aattgctgca acagttgccc tttgctggtt
    13081 tccccctgca acaaaacggg aagcgtcatg gtagcgcgga actgctcggc cagatcacgc
    13141 atcatggcgt gcgaggtaca aagcataaaa caacgaccgt tgttagcttc gatgatcggt
    13201 cgcagcattg ccgctaactg gcgagcagaa cctggctggt tggtttgcgg cagattgcgc
    13261 ggcacacaga gtaacgcctg gcggctgtaa tcaaacgggc ttggcaatag caacgactcc
    13321 gcctgttcta tcccaagccg cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt
    13381 gctgaggtga agatccagct accaggcttt tgcgccatta actctttgaa tttatccgcc
    13441 acgctgagcg gcgtgagagc cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg
    13501 tagcccggct gattgatctc ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc
    13561 tcaaatgccg catccagcaa ggcggaacgc cccagtgaca gtttcgccac gtcataacaa
    13621 agttccaggg tgtcatcgag cagtaaaaat gcccgctgaa tttgcggatt agctaacagc
    13681 tcacgcaggt taccgcgata acctggctca ccgagttgca gacgaaaatc ctgcgcactc
    13741 tgggcaagac gatcagcgca cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa
    13801 gcgatggtga tgtcttttgc caggtcgagc agttgtcgac tggagagtga ctgaccaaaa
    13861 tactggctgg caatgtccgg tagctggtgg gcttcgtcga agatcatgac gtccgcttcc
    13921 gggatcaact cgccaaatcc actctcttta accaccatat ccgccagaaa gagatgatgg
    13981 tttaccacca ccacatcggc gtccatcgct tttttacgtg ctttgaccac aaagcaatct
    14041 ttatacatcg ggcagtcgct gccaagacag ttgtcgttgg tgctggtaac cagcggccac
    14101 gcctgtgaat cttccgccac gctgacgcag gtgctgatat caccatcgac tgtttgatta
    14161 gaccaggagc gcagcaggat cacatcgctt aagatttgta ccggcagatc gccccccgcc
    14221 agcgcctgct gttcgagacg ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc
    14281 acgttgcccg tatatttcaa tgcctttgag actgttggca aatcgcggct gtagagctga
    14341 tcctgcaacg cttttgagcc ggtcgagata atgactttct ttttcgcccg cagcgcagga
    14401 gccaggtaag cgtaggtttt gcccgtaccg gttcctgctt ccaccaccag cggctggcct
    14461 ttttctatcg cctgggtgac ggctaccgcc atctgtcgct gtggttctcg cggcttaaag
    14521 cctggtatcg ctttcgccag ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct
    14581 ggtgtgttta ttttgtggtt tactcttccc ccgaaggctc tgacgggaac catgcagcct
    14641 gcctccccct ggggttacaa gcccgctcaa cagcatattg cgcatggttt ctgctcttat
    14701 ccaagcccgt attgttgact gaaccaggtg caaagttcgc acaccaggaa gggataaata
    14761 agatgtcaga cagtgtaaca gttggtatcg atgtatcaaa acttactctt gatgttcatg
    14821 tgtctcctgc acagcttcgt cagactctat caaactccga cgctggcttt gaggagctga
    14881 aaacattgct ttccatgcag caggtttctc ttgttcttct cgaatccacg gggggttatg
    14941 aggatgccgt tgtctgcttc ctgcagtctc ttggttatga cgtttctgtc attaatcctc
    15001 gtcaggctcg tgattttgcc cgctctatgg gacgtctggc taaaaccgac aggattgatg
    15061 ctgagctgct tgcccagctt gcttctgtta ttgattcccg ccctgaccgt gcgcgttttg
    15121 tcaaaaaaat taccgatgac aggcgtcgta agctggcctg catggtcacc cgtcgccgtc
    15181 aggttgtgaa tatgatttcc actgaacagc agcgtaaatc tgccgccagt gtgtattcca
    15241 ctgaaagtat taatagggtt ttatcttttc tgcgctctga gctgaaaagt attgatgctg
    15301 atatttcact gcatgtccgc tctcattttg cagatatttc cggtcttctt gcctcattta
    15361 aggggattgg tcctgccaca attggcgtac tgctgggtga ggttcctgaa ctgggccgac
    15421 tgaaccgccg tcagattagc gcactgattg gcgtggctcc gtttaaccgt gactccggca
    15481 ttatgcgcgg tcgccgtacg gtatggggcg gtcgtacaat ggtccggaac gcgctttaca
    15541 tgccaacgct ttctgccgta cgttttaacc cggttatccg ggagttttat acgcggctga
    15601 tttctcaggg taagcccaga aaactggctc aggttgcggc catgcgtaaa ttgctctgca
    15661 tcattaatgc catgctttct tcagggacaa tgtttaaccc tgccctgcac ctccattcag
    15721 ttcaggaggc tcaacaatga aaacggaatc agccatcctc acagcctata acgccatcga
    15781 cgcggcatcc gttgctctcg gtcgtccttg tctgacactg cccgaacgtg agcattttca
    15841 ggctgagcgc gatgttgcca ctcgcaacct tgcgacgctt tcccgtcagt atcgtgatga
    15901 ggtcggggcg ctctcttcac agcttgccct gccacgtaac acgcaggttg accgtgtgat
    15961 ttacggaatt aactgcacgg atttatccct tgctgacagg ggcagggccg ccggactggt
    16021 atcacagttt ggctcgtttt ccttcaccgt ggcgcacgct ttctgccgtc tggtttatca
    16081 gctcggtgtg aaaaattcgc tgatggcgct tgagtctgcg caccgtttcg ctttctttaa
    16141 tcaggacggg gacccttatg aattctcgct tttctgcact gatgagcagc ttgctgaggt
    16201 ggctgattcg gttgttcgtg actgccttgt ttctcgggcc atttatagtg atgtaccaga
    16261 gcagcacctt ctcaatgtga tggtgtattt tcaggccata tccggctttg atttcgcgcc
    16321 cgtttatgca acttatcatg agcgttacgg cgatgagggg ttgcttaagc gtctgactga
    16381 cctggctttt gtcacgcgtt tccttcgggt tgtccgtgac cagcgggtca atgaggtctg
    16441 tcgtatgctc ggcatactga accgcaccac gccttatatt tccgactggc accgcgacct
    16501 gtttgctgtc cgcagtaaac gcgtgaaaaa gtatctccgg tcctccggcg tctttgatgc
    16561 gtttaatgag ctggtctgta cgctggaaga tgcgcataac gcctctgttt cgaacccgaa
    16621 aaaccggatt gctgaactct gcgttcgtgg aaaggccgtc tgtgaactgt ccgaagatat
    16681 ggggctgtcc ggctacttca tcgtgctcac cacgccgtca cgttttcacc cgacgaccag
    16741 ttttaaggtt gccggaaaat ggcattcccg cccgaacaaa aaatggtggg aagctggttg
    16801 tccgaccatt aaggattcgc acgcctggct gaataccgtc tggcgtcggg tctgccgcag
    16861 gctggataaa gccggtattc agatacccgg tctgcgcacg gttgagccgc atgctgacgg
    16921 tacaacgcac tggaatttcc tgatttactg caatccccat gagagcgcaa cggtgctggc
    16981 cattttccgt gaagaggcca tgcgcgatga gccgg
//
LOCUS       DAHBHC010000102        16056 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHBHC010000102 DAHBHC010000000
VERSION     DAHBHC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.103"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..729)
                     /locus_tag="M2A35_004252"
     CDS             complement(<1..729)
                     /locus_tag="M2A35_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
                     /protein_id="HCB7200475.1"
                     /translation="MGDYTNTGVEAWKPNFYIAADDVNITEKIRKGLINITLTDYGGS
                     SKQTDEIRVAIVSETLKIPARGVKITLGLGFGNQIIDKGVYVVDGASSGGEPRVVDFT
                     AKAAPMNAAKGAKTVQSKKTRSWIDQTVGDIVAKIASDNGLNPRVSTRFADTKIEHID
                     QIGESDMHFMSRLATRFDAVSKPAGGYWIFLPRGKGESVSGAPLQHYTLKRAGNSSWG
                     YSRNGQSGDSGGGGENPEPTYLIKY"
     gene            complement(733..948)
                     /locus_tag="M2A35_004253"
     CDS             complement(733..948)
                     /locus_tag="M2A35_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCB7200476.1"
                     /translation="MTITYTTRDGDRLDQICLAVYGRTRVTTETVLYQVSNYGVTDMC
                     AVFPAGQIITLPEIEPEPIKKETQLWD"
     gene            967..1209
                     /locus_tag="M2A35_004254"
     CDS             967..1209
                     /locus_tag="M2A35_004254"
                     /inference="COORDINATES: protein motif:HMM:NF025731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HCB7200477.1"
                     /translation="MEIFTWIVALYIWLMANPLASIILYVIPLIIALMRGHKSCTMVV
                     ILNVLFGWIFLMWIILILWAAFGDKKPKDIQSNEAP"
     gene            complement(1188..1577)
                     /locus_tag="M2A35_004255"
     CDS             complement(1188..1577)
                     /locus_tag="M2A35_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7200478.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDSWRWAEQTRFRKNDALQMTGR
                     PNPTITIDGKINALFLDGCGIGLLSDLRALGNEGNPQQLVLGTGKVMGYWVIKDLNET
                     QNSFFMGGTPKTQDFTLTLQYYGASLD"
     gene            complement(1603..3232)
                     /locus_tag="M2A35_004256"
                     /pseudo
     CDS             complement(1603..3232)
                     /locus_tag="M2A35_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3338..3622)
                     /locus_tag="M2A35_004257"
     CDS             complement(3338..3622)
                     /locus_tag="M2A35_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCB7200479.1"
                     /translation="MESLLDSMTVTLSRPFEINGVTVETITVREPKLRDRILFSKDKG
                     DAEEKTARMLARLANLNIEDIYSLPSCDYMKMEEAFNELVKIPTDRKQIL"
     gene            complement(3636..4148)
                     /locus_tag="M2A35_004258"
     CDS             complement(3636..4148)
                     /locus_tag="M2A35_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCB7200480.1"
                     /translation="MSRGSVYRAFTLWVQGERICNCTEYTPVDMKLIEDDFKTGAMDQ
                     GITLDGGMEKMAASFKVTGSDTDVMAYFGIIPGAKTRFEIRAAYTDNYGISFERIDIY
                     EGLITAITDDAMGTDSKAAVGSTVNIAPSYYKRTQNSKLIYELHPAKLKRVINGVDVL
                     AGVASILHVY"
     gene            complement(4163..5674)
                     /locus_tag="M2A35_004259"
     CDS             complement(4163..5674)
                     /locus_tag="M2A35_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCB7200481.1"
                     /translation="MSTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSAGAADA
                     ELITGSEILKNRVKFTATNQGARGNSWSVEIIASETEGGNSLWRDMPDGSRRLTIYVS
                     APSGLHSYTPDEIVQNVTIPNPPSDQIFLTVTAVDTGSTAGAVTASPLQYLSGGSDEA
                     YPINTPALIAGSPKKAALLGERGTLPKDIADIFNQDNALLVVVRVKDDKDATKQQQNI
                     IDGVKQLEKSKQLTGVTPRIIIAPDFSAIDTVAAQVEATATKLRGVGYIDSPTAATAE
                     DVVRRRQSYGARVEILRPRIFTTSDITGASRAYSATAAGLRARIDNDGKHGFWWSKSN
                     QNIYGVTGVEQIDDFIIGETNCTANLLNAEQVSTIIRYDGFRHWGNYLCSLDPQWSFE
                     CVRRTADVIEDSIAIAMMNDFIDRPIDVHLGGDIIETINAYIRKLYEMGAINGGRAWL
                     DSELNTKESFAAGKIYINVEFAPKSPAQTITITYRINNDYTVDEFSSLLKQAA"
     gene            complement(5687..6265)
                     /locus_tag="M2A35_004260"
     CDS             complement(5687..6265)
                     /locus_tag="M2A35_004260"
                     /inference="COORDINATES: protein motif:HMM:NF014467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7200482.1"
                     /translation="MQNLKSFKLVKPKTKLLKLSESDGQNILYLQSEDGMDWYECQSL
                     YRDDTVKIMYDKENIIRSVVDRPVPERGNTYSVSMFFPIDMSVAEVELSTYPAELTLD
                     GSWKFVDGDVYQDEQIVNERIYKENSKKINELVREAQSAILNLQTAIEIDMSSDGDEE
                     KLIEWRRYLCELNKITATDLSQSPLNTPLRPA"
     gene            complement(6826..7392)
                     /locus_tag="M2A35_004261"
                     /pseudo
     CDS             complement(6826..7392)
                     /locus_tag="M2A35_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829620.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(7385..8011)
                     /locus_tag="M2A35_004262"
     CDS             complement(7385..8011)
                     /locus_tag="M2A35_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCB7200483.1"
                     /translation="MTSQSILPPNANAAERALEAVLSHAGDLPADIRIIKNPDLCPVD
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPKDDPYTFAIKIYLLKGMGLDLDLFNTFIAQIFDAKNCRSLLKEINFETGVDG
                     EFFIAGAPYAKIGVNIPADGDGGVKINGGLYISGSPIVSLNVEIGPNG"
     gene            complement(8014..8934)
                     /locus_tag="M2A35_004263"
     CDS             complement(8014..8934)
                     /locus_tag="M2A35_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCB7200484.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPDFVITPLFSDVKSELLAELQQL
                     YPVFNALLESDPAVKLLEIVAYWRIIVTARVNQGMLAVLLAFAKNSDLDQLGANFDCE
                     RLLITPANPDAIPPVEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDVYVLSRTGDGVPPQSLLNTVNSALSPDDVRPLTDFVTVKPAAN
                     LNYQIEAIIVAGLGPDQNVLLNGAQADLETYVKQQHKIGATAALSGIYDSIHRDGTER
                     VILTSPAADVIAGVGQAPYCTGIKLSVQMG"
     gene            complement(8931..9272)
                     /locus_tag="M2A35_004264"
     CDS             complement(8931..9272)
                     /locus_tag="M2A35_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCB7200485.1"
                     /translation="MQGMNRSDGKSLAGTDHICQSIVDILTTPFGTRVMLPEYGSNIF
                     DLIDNPSDPSLAMRIIMATAGALAKWEPRIQVDSVNVAAIDIGKITISIIATDVETRN
                     RLEFKNMELIY"
     gene            complement(9280..10188)
                     /locus_tag="M2A35_004265"
     CDS             complement(9280..10188)
                     /locus_tag="M2A35_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCB7200486.1"
                     /translation="MAQSDDDSEYTTAENARRQRDAIKRGTIAEVKMSPPRCRVSFGG
                     EHKSGWLQWFTHATSERVDWSAPSVGDPVTVISESGDTRNGVAMLGLHIDNKPPPSSD
                     PHDHVTAYCDGATLTYNTENHTLTWQGVPDGIVKILGESKIEILGRADVTVTSENVVN
                     IHGGKLINADAEVINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPG
                     GITLAGPTHITETLVVDKLATFRNDVSVTGDNGGTGNITTRGSVLAGQEVQDRQGTIT
                     EVRTTYNGHTHTCPDGETDNPNQPMA"
     gene            complement(10169..10717)
                     /locus_tag="M2A35_004266"
     CDS             complement(10169..10717)
                     /locus_tag="M2A35_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200487.1"
                     /translation="MSGKISGMDALDEYTNNVHAALMQIPFIKTVGIYPEIPDGFITP
                     AIFFEVESWSASNDVIAGSAMTVELTCNLYLVREYAADLYSQKARNAALFVSGWINDR
                     IFGPMTRPAVFTDAQEGDWQKSGKSVASHSVWCVSFKQNVGVGADPFDYPSDGILKQV
                     FVGFAPEIGAEHESDYYGPIGR"
     gene            complement(10714..11397)
                     /locus_tag="M2A35_004267"
     CDS             complement(10714..11397)
                     /locus_tag="M2A35_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200488.1"
                     /translation="MANPRSNAQMFDIDMSALTGLRDAVEATEHQMIMAYNRALNRTA
                     KHMHRVSVSMIIDALGLKSRKVANKRTQQFIKRRDYSKEGAGDLSSVKLWYGLDDFRI
                     HNLRGTMRQPRTGKQERDPETGQFLKKKKTVAGASFTPKSSSLSAMGWPDSFVATRFG
                     YRSIWKQVNGGRNIEEMRIPVHEALEDAIDDYIYQNIGPVFMAYYEQDLRGRVAGSVH
                     VNAKTGKRL"
     gene            complement(11432..11761)
                     /locus_tag="M2A35_004268"
     CDS             complement(11432..11761)
                     /locus_tag="M2A35_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200489.1"
                     /translation="MRRADKTLVETFNVDNGVTLFPDTPQEITVQGVYDAPFFGVDIA
                     GGRISSSDSCITLHDYDCVNLKKRDTIKIIGKLWTIREMQPDGTGLTDIYLDPKTTSE
                     NSVYSKY"
     gene            complement(11782..12108)
                     /locus_tag="M2A35_004269"
     CDS             complement(11782..12108)
                     /locus_tag="M2A35_004269"
                     /inference="COORDINATES: protein motif:HMM:NF021473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HCB7200490.1"
                     /translation="MAKNYIEDGKTLDWTNTGANAVNSGDAVGVGAVVGIAHDNIAAS
                     ATGVLHMSGVFELPKTSTDSWAQGVKLYLASGKLTTTSSSNPLVGVAWAAAAAGSATA
                     VVRLGG"
     gene            complement(12148..14217)
                     /locus_tag="M2A35_004270"
     CDS             complement(12148..14217)
                     /locus_tag="M2A35_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCB7200491.1"
                     /translation="MTLAPRASAPARTGAGDGWYEVRAAANGAPAEILIFEEIGAWGI
                     SARRLINDIADADLFSAPAVDIRIHSPGGSVLDGFAIFNTLRRLTGVVNIYIDGLAAS
                     MASVIAMLPGAQVHIPENAWVMIHNPWGGAWGDAAELREYADLLERHEKNIVNAYVNK
                     TGIDAEEVKTLMSAETWLTGAEAVEKRFADVLVPKVEMAACINDNVTKEFSHMPQAAQ
                     KFFKPRATGTQPQPQPAAPQPSPQATQQPAAAQPAAPQPAAQDAAAIATQALEMMAAR
                     ETARKAAVSAVFTNFPAFATLQNTCLTDTTCTDAVARQRLLDALAQGAAPAGGAHIYA
                     GNGNLVGDSIKNVIMSRAGYEEREGSNNYSGMTLAELARASLADRGIGISGAGRMEMI
                     GMAFTHSSSDFGNILMDVAHKSALAGWDEAEETFDVWTRKGELTDFKTANRVGLDSFP
                     ALREVRPGAEYKYVTLGDSGATIALATYGELFSIDRQAIINDDLSMLTRIPHAMGTAA
                     KATIGDLVYALLSSNPKFGDKPLFHTDHGNFIDSPLSAAALATGRSMMKKQKNGKRHL
                     NIRPAFLLVSTANESLADQIINSTSVPGADNNSGISNPVRGMAQIVADPRIDDAGEKN
                     WYLAAAQGRDTIEVAYLDGNDAPVIESTSGFTVDGVTMKVRIDAGVSPMDYRGLLKST
                     GDGREGE"
     gene            complement(14168..15706)
                     /locus_tag="M2A35_004271"
     CDS             complement(14168..15706)
                     /locus_tag="M2A35_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7200492.1"
                     /translation="MGIFSRLTTAIAPVTSLKRARARIQLDAIRAYEAAKPSRTRATK
                     RETRSADNAVFAAGVSLREQARWLDENHDLAIGVLDKLEERIVGPHGIQIEPQPLTLA
                     GDVHEDFAALLASRFAKWAENPDVTGMFTLAEAERILLRSALRDGEVFIQLLRGAVPD
                     LKYDTPEQFALELLEADFVPMWPSGADGSNRIIQGITVNRWGCPVGYCVYKNHPGAGF
                     STDTRQIPAENMLHLAVRKRLHQVRGVSLFHGVMSRLGDLKSYEDAERVAARVSAMLG
                     FYIKRGDASVYGDDADWTSPDNSYRDFEMAPGMIYDRLAPGEELQMLSSDRPNPNLMD
                     FRNGQLRALAAGVRTGYSSIARDYNGTYSAQRQELIEAGDGYAVMQNWFISRVVRPVY
                     REWLKMFLLSGVEVPADVDPESLFDAVYMGPVLPWIDPQKESEAWKAQIRGGAATEAE
                     WIRARGRSPLAVKLQRRREIKFNKTAGLVFDTDPANDNGSQSNDAKNDAGTASQRPGE
                     NRGG"
     gene            complement(15709..15921)
                     /locus_tag="M2A35_004272"
     CDS             complement(15709..15921)
                     /locus_tag="M2A35_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200493.1"
                     /translation="MEISKIKSARDRAFAAYQDALEAQSLGMKGRTLTRQNISALRSE
                     FEYWDKRLSTATRGGGRQFSLVRFRE"
BASE COUNT         3652 a         4256 c         3646 g         4502 t
ORIGIN      
        1 atatttaatc agatatgtgg gttcggggtt ttcaccgccg ccgccgctgt caccgctctg
       61 cccgtttctt gagtaacccc agctcgagtt accagcgcgt tttaacgtat agtgttgcag
      121 cggtgcgccg ctgactgatt cacctttacc gcgcggtaaa aatatccagt atccgccagc
      181 gggcttactg accgcatcaa accgggttgc caggcgtgac ataaaatgca tatcagactc
      241 gccgatctgg tcgatatgct cgatttttgt atctgcaaac cgggttgaaa cgcgtggatt
      301 cagaccgttg tcgctcgcaa tttttgcgac aatatcgccc acggtttgat caatccagga
      361 ccgcgttttt ttgctctgta ccgttttcgc gccttttgca gcgttcatgg gggcggcttt
      421 tgcggtgaaa tcaacaacgc gcggttcgcc accactgctg gcaccatcaa cgacgtaaac
      481 gcctttatca ataatctgat taccgaaccc cagccccagc gttattttta cgccgcgcgc
      541 ggggattttc agcgtttctg atacgatagc gacgcgtatt tcgtcggttt gtttactcga
      601 tccgccgtag tcggtaaggg tgatattaat aagccctttt ctgatttttt ctgtaatatt
      661 tacatcatcc gcagctatgt aaaaattagg cttccacgcc tcaacgccag tgttagtgta
      721 atcacccatt catcagtccc ataattgagt ttctttttta atcggttcag gctctatttc
      781 cggcaatgtg atgatctgcc cggcagggaa cacagcgcac atatctgtta cgccgtagtt
      841 tgaaacctga tataaaacag tctctgtagt aacgcgggtt cgcccgtaaa ccgcgaggca
      901 tatttgatcg agccgatctc cgtctcgcgt cgtatatgtg attgtcataa taatgaggtt
      961 ccaagtatgg aaattttcac atggattgta gccctgtata tctggctcat ggctaaccca
     1021 ttggcgtcca ttattttata tgttattccg ctaatcattg cgttaatgag agggcataaa
     1081 agctgcacca tggtagtaat attaaatgtt ctttttggct ggatattcct gatgtggatt
     1141 atattaatat tatgggccgc tttcggcgat aaaaagccaa aagatattca gtctaatgaa
     1201 gcgccataat attgcagggt taacgtaaaa tcctgcgttt ttggggtgcc gcccataaag
     1261 aaactatttt gcgtttcatt caggtcttta ataacccaat agcccatgac tttaccagtc
     1321 cccagcacca gctgctgggg gttgccctca ttacccaggg cgcgcaaatc tgataataac
     1381 ccaatcccgc aaccgtctaa aaataaggca tttattttac cgtctatagt gattgttggg
     1441 ttcgggcggc ccgtcatttg caacgcgtca tttttcctga atctggtttg ttcagcccag
     1501 cgccatgaat cactacgctg taatttattg taggcgactg ttgatatagc aaaaataaaa
     1561 tcacccaggc cgagcattat atcctgtgcg tcgttcattt atttaccccg ccgccactgg
     1621 tctgtccatc attgatccgc gaccgccaga tggcgcggcc tgtcttattc cgtctaatac
     1681 ccctgacgcc gccgccgcct ggatagattg agggtcacca gccgccataa tattaacctc
     1741 aattttatta ttttgttgcg gcgctggttg cgccacggct tgttttagcg catccatatt
     1801 tatagttgca ttttgcagtg aatcaggggc cgccgccgct ttttgtgcct caacaaatgg
     1861 attagccagc gacatgatcg cctcggcatt ttttttacgc tccggatgac gcatattatc
     1921 gacggcttct tttgctttag cttgtgcaac attatcagca gccaggccag cgttcggtga
     1981 atattttccg cccgagtcca tgaattcttg atacgctttt gcgtattctt ctttatatac
     2041 tttttcttct tcttcggtct tttcgtccgg aattaaccac tctaattttt ttaccaccac
     2101 ccatattatt ttaccgaatt ttacgcagcc ctcgccgaaa ttaactatgc catcccacag
     2161 ctttttcatg ttctccggtt tcacccactc attttacagt attaacaata gtcccttgat
     2221 tgtctttaaa ccatgatgta aatccctcgc gcatcgagtc aaatgtcggg gccagtttcc
     2281 caccaatttt cccgagcgtg tcggcaaatc ccgaggaaat agaaccccat aaattagtta
     2341 atgagaaatg cgcgcgggcg gctccctccg cgccctcctg cgttaataaa ttggattttt
     2401 tggcgtcttc catggtctgc tcccatgttt tgccagtaat cctcatatac gtcattattt
     2461 tatttgcctc accgcccatt aattggtcgg cgagacttgc ggcctgtttt tcgtctttca
     2521 ttttcgaaag acgactcatt acctcgttaa attgtttttc ccgatcccag ctctgcatcc
     2581 tttttttggt aagaccgatc tggaacagca tcgggttaag gtttttttcg ttgccgacct
     2641 cacccaattt atttgttaat tcctcggata aatcaccaac gttttcaccg ttgaggccag
     2701 ccattttacc gacgttatcc cacgccgcat atttttcgat tcctacaccg taggaccggg
     2761 ccagccctaa tttttcggcg gtttcagcat tcattgcggc ggcaccagct ccggcggcac
     2821 caattagccc aatcccgcca agcgcgcccc atttaacgcc gctgccgacg gcttttgcgg
     2881 tgccgagcgc agctctaccg ccccatttcg cagcgccctt tattttgctg ccaatgtttt
     2941 caattcgctg tttttttgct aactgtttat ttaattgctc aagatctttc gtaacgatat
     3001 taatttcacg cccgactttt tgatattcgt gaaccagact tttagtactt ttgcccgcaa
     3061 gctcggtttt tttgatctgg tcttttaatt tctcctgttt acgtgttaac gttgaaacgg
     3121 tgttacccgt ctgctttata gcgccgtcca gttcgtcggc gctgcgctta aaagaggggt
     3181 ctatgcggcc cccaaataca acttttgttt taaattcctg tgtagcagcc atcttttttc
     3241 attagctctg ttacgtagaa attaaaaacc cgatacggca tatttagttg cgtatcggga
     3301 ggaatagcca gctttttagc tatatacggg agcaggatta taatatctgc ttccggtcgg
     3361 tcgggatctt taccagttcg ttaaatgctt cctccatttt catataatca caggaaggaa
     3421 gggagtaaat atcctcaatg ttaagattag ccagccgtgc cagcatacgc gcagtttttt
     3481 cctctgcatc gcccttatct ttgctaaaca agatgcgatc gcggagctta ggctcgcgca
     3541 ctgtgattgt ttcaacggtt acgccgttaa tttcgaacgg acgcgataat gttaccgtca
     3601 tgctgtctaa taatgattcc atttttattt cctgattaat atacgtggag aatacttgca
     3661 acacccgcaa gaacatcaac gccattaata acgcgtttca gttttgctgg atgcaattca
     3721 taaattaatt tactattttg agtgcgctta taataactcg gggcgatatt aacagtgcta
     3781 ccgactgcgg cttttgagtc agtccccatc gcgtcatcgg taattgcagt aatcaaccct
     3841 tcgtatatat caatgcgttc aaaactaatg ccgtagttat cagtatatgc ggcgcgaatt
     3901 tcgaaacgag tttttgcacc ggggataatc ccaaaataag ccataacatc agtatctgac
     3961 ccggtgactt taaaactagc ggccattttt tccatgccgc cgtcgagtgt aatgccctga
     4021 tccatcgcac cggttttaaa atcatcctca attaatttca tatcaaccgg ggtatattcg
     4081 gtgcaattgc aaatacgctc accctgtacc cacagtgtaa acgcgcgata aacgctacct
     4141 ctggacatat ttaaccccta tgttatgcgg cctgtttcag caatgaagaa aattcatcga
     4201 cggtgtaatc gttattaata cggtacgtaa tcgtgatggt ttgtgcgggt gatttgggtg
     4261 caaattcaac gttaatataa attttcccgg cggcgaaact ttctttcgta ttcagctcac
     4321 tatcaagcca cgctcgacca ccgttaatag cgcccatttc gtatagtttg cgaatatacg
     4381 cattaattgt ttcgatgatg tccccgccga gatgaacatc gatcggacgg tcgatgaaat
     4441 cattcatcat tgcgatggcg atcgaatcct caattacgtc agctgttcga cgaacgcatt
     4501 caaacgacca ctgcggatcc aggctgcaca gatagttacc ccagtgtctg aacccgtcgt
     4561 aacgaataat tgtgctgacc tgttcggcgt ttaacagatt agccgtgcaa ttggtttcgc
     4621 cgataataaa atcatcaatc tgttcgacgc cagttacgcc gtaaatgttt tgatttgatt
     4681 tactccacca gaaaccgtgt ttaccgtcgt tatcaatacg cgcacgtaac ccagctgccg
     4741 tcgccgaata tgcgcgcgat gcgccggtta tatcgctggt agtgaaaatg cgcggtcgca
     4801 ggatttcaac gcgagcgcca tagctctggc ggcgtctcac tacatcctcc gccgttgcgg
     4861 ctgtcggtga gtcgatatag cccacgccac gcaatttagt tgccgtcgcc tcaacctggg
     4921 cagcaactgt atcaatcgcg ctaaaatctg gtgcaataat aatacgcggc gtgactcctg
     4981 ttaactgttt cgatttttcc agttgtttga caccgtcgat aatattttgc tgttgttttg
     5041 ttgcgtcttt gtcatctttt actcgcacaa caacaagcaa ggcgttgtcc tgattgaaaa
     5101 tatcggcaat atctttcggc aacgtgccgc gttcacctaa taaagcggct ttttttggac
     5161 ttccagcaat cagtgcgggg gtattaattg ggtatgcctc atcactgccg ccgcttaaat
     5221 attgcaacgg tgaggcagtc acagctcctg cggtcgaccc tgtgtccacc gcggtgacag
     5281 ttaaaaatat ctgatcagag ggagggtttg ggatcgtgac gttctgaaca atttcgtccg
     5341 gtgtgtagct gtgtagtcca gagggtgcag aaacatatat agttaatctc cgactgccgt
     5401 cgggcatatc ccgccacagt gaatttccac cctctgtctc gctcgcgata atttccacgc
     5461 tccatgaatt accgcgcgca ccctgatttg ttgcagtaaa tttaactcgg ttttttagaa
     5521 tctcgctccc ggttattaat tccgcgtctg ctgccccggc agaatccggt gcagtaccaa
     5581 caatcccgat aactgaaata tcgaccgtgt tgatctcttt tgtgccgtcg tcgtattcga
     5641 gtgtgcgaat accgtgtaaa aaattagtgc tcattaggta ccctatttat gctggtcgta
     5701 aaggagtgtt aagtggggac tgacttagat ccgtggctgt tattttattc agctcgcaca
     5761 ggtagcgtcg ccattctatt agcttttcct catcaccatc tgatgacata tcaatctcta
     5821 tggctgtttg taaatttaga attgctgact gagcctctcg gactaattca ttaatttttt
     5881 tggagttttc tttgtaaata cgctcattta caatttgctc atcttgatat acatcaccgt
     5941 caacaaattt ccacgatccg tcgagagtta gctcagcagg ataggtgctc aactcaacct
     6001 ccgcaacact catatcaatc gggaaaaaca tgctcacaga ataggtattt cctcgctccg
     6061 ggaccgggcg atcaacaact gatcgaataa tgttttcttt gtcgtacatt atttttactg
     6121 tgtcatcgcg atacagtgac tggcattcat accaatccat gccgtcttcg gactgaagat
     6181 ataaaatatt ttgtccgtca gactcgctaa gtttaagtaa tttcgtttta ggttttacaa
     6241 gcttaaatga ttttaaattt tgcataactc atccggaaat cgtagtccat acattattaa
     6301 tgtatttttg gatcggttta taataaacac cgccgatatt atcagctgag ctgtcgccgg
     6361 tttcctgaac atatatccca gtaagaacac agccggtcgg acatttaaag tcccacgtaa
     6421 cctcattacc cgccggtgtg tgggcaacct ctgcgccaag ttgaacgttc tgtatccccg
     6481 agctgcttac ggttattgcg acgttcccgt tagcatctgg cgacttcccg ttaatcgttt
     6541 taaccggttt aagatttccc tcattccaat atttatacgt ctgcccgccg cacagtaaca
     6601 ccagaccatc atagtgaacc ccgcgtaatc tataaaaaac gttttgatta ttttcccccg
     6661 cgtcgacact tctaaataat caccgttcac atctgaccag cccgcaagtg aaaataatgt
     6721 taagttggta tctggtttat ttatgtttat ttgtttttta gaaatcaaaa tgtcttcaaa
     6781 aaaaacgtcg tctttaaagg tgttttgagt tgtaaatgta ttacccttat ctctatatgc
     6841 aacgtcccca tctataccaa tatttttccg cgcagcctgc tgagcagaac caccattagc
     6901 ggcaatttca gataaatttt tgcttatctg caaatatttt ttgtcagcta cctcgggagt
     6961 tacgtagttg tcgtatacca caacattaac tgtagatgtg ctggacaccg cgagggtgta
     7021 tttatacgtg attgaaatca gtgcgccgtt actgtccgta ggttttaaaa tatccggtga
     7081 acgtgcaact gagtacagtt cgcctttatc ggttaatatt cccacctcac ggatagtata
     7141 accgccagtt tccggtggta cgtatagcgt aaaaataacc tggttaccgc tattttctgc
     7201 ggatttaata tccccgcgat acgtctcgtg aactaatgca gtacgcgccg gatccggtgt
     7261 tacgtcatcg tttccgttcg cgtcgccgat aacaaatttg ttcagcacca ctggaacgcc
     7321 tgacgccagc gcggcggctt ctaactgcgc gccacggtca gttaacacag cgtaaatttt
     7381 attttcagcc atttggccct atctccacat ttaaactaac aattggtgag cctgatatat
     7441 ataggccgcc gttaattttt acaccgccat cgccgtctgc cggtatattt acgcctattt
     7501 ttgcataagg tgcaccagct ataaaaaact caccatcaac gcccgtttcg aaattaattt
     7561 ctttaagtag ggagcggcag tttttcgcat cgaatatttg agcaataaag gtattgaata
     7621 agtcaaggtc taaccccatg ccttttagca aatatatttt gattgcgaac gtgtacggat
     7681 catctttcgg ggtggcttca aaccactccc gcattttgct ctcaaatcct accgttaata
     7741 acgctcgttc tacggcacca cgcgtacccc ggtgtttgtt ttgccaggcg gcggctttaa
     7801 taatttcgcg tttttgttgt tcgctccagt ccggattcca gtacgtgacc gcgtattccc
     7861 acgccagcca gggtaataaa tcaaccgggc ataaatcagg gtttttgata atgcgaatat
     7921 ctgcgggtaa atctccggcg tgtgataata ccgcctctaa agcccgctct gcggcgtttg
     7981 cgttcggggg taatatgctt tgactcgtca cattcacccc atttgcacgc tgagtttaat
     8041 accagtgcag tagggagcct gtcccacgcc tgcaatcacg tcggcggcgg gtgacgttaa
     8101 tattactcgt tccgtgccgt cgcggtgaat cgagtcataa ataccggata atgctgccgt
     8161 cgcccctatt ttatgttgct gtttaacgta ggtttctaaa tcagcctgag cgccgtttaa
     8221 taacacgttt tgatccggcc ccagcccggc aacaataata gcctcgattt gataattcag
     8281 gtttgccgct ggtttgacgg tcacaaaatc agttagcggg cgtacgtcgt ccggtgatag
     8341 tgcggaattg acggtgttta atagtgattg cggcggcaca ccgtcgccgg tgcgcgataa
     8401 cacgtaaaca tcaacgtaac cgggctgagt aaccggcggc ccatacgcct gcgcggataa
     8461 tacgtccgga tcactggata gcgcgaaata attatacgcg ttagtgctgc ctgcggtgtt
     8521 ccgcgcgtac cacgacaact ggatccggtg ccggtattcg tcgtcgctct cgtaaacagc
     8581 ctcaacgggc gggattgcgt cggggttcgc cggagtaatt aacagccgct cacaatcgaa
     8641 atttgcgccc aactggtcca gatcgctatt tttagcgaac gccagcaaca cggctaacat
     8701 accctgattg acgcgcgcag taactattat tcgccagtac gcgactattt ccagcaattt
     8761 tactgccgga tcggactcta acagcgcgtt aaatactgga tataattgct gtaattccgc
     8821 taataattct gatttcacgt cggaaaatag cggggttata acgaaatcgg gagggggtag
     8881 caaactcatg tcgatagttt ttactatcga gttttgaaca gttgatgtta tcaatatatc
     8941 aactccatat ttttaaattc cagtcgattt cgggtttcga cgtccgtcgc aataattgat
     9001 attgttattt ttccaatatc aattgcagcc acatttacgc tatctacctg aatgcgcggt
     9061 tcccattttg cgagcgctcc agccgtagcc ataataatgc gcatagctag cgacggatct
     9121 gacgggttat ctattaggtc aaatatgttg ctgccatatt ccggcagcat aacgcgggtt
     9181 ccgaacggcg ttgttaaaat atctactatt gattgacaga tatgatcggt ccccgccagc
     9241 gactttccgt cgctacggtt catgccctgc ataatttatt tacgccattg gttgattggg
     9301 gttatctgtt tcgccgtccg ggcatgtgtg cgtgtgtccg ttatatgttg tgcgaacctc
     9361 agttattgtg ccctgacgat cctgaacttc ttgccctgcc agcacactgc cgcgtgttgt
     9421 tatattaccc gtgccgccat tatcgcccgt aacagaaaca tcattgcgga atgtcgccag
     9481 tttatcaaca accagcgttt cggtgatatg cgttggcccc gccagggtaa tccctccggg
     9541 tgcccaggcg ctgattgttt gtgctgtcag cgatattcta tttgcagcag tgacactcac
     9601 tgagctggta gcaataacgt taaccagatc cgcgtgtgcg ttaatcgtgt ccgtagcggt
     9661 gacgtttata acctctgcgt cagcgttaat taattttcca ccgtggatat taaccacgtt
     9721 ttcgctagtt accgtgacgt ctgcgcggcc caaaatttct attttggact cgccgagaat
     9781 ttttacaatt ccgtcgggta cgccctgcca ggttagcgtg tgattttccg tgttataggt
     9841 tagcgttgca ccgtcgcaat atgccgttac gtggtcgtgc gggtcggaac tgggcggcgg
     9901 tttattgtca atgtgcagcc ccagcattgc cacgccgttg cgcgtgtctc cgctctctga
     9961 gataacagta acggggtcgc caaccgacgg ggcgctccag tccacgcgct ctgacgttgc
    10021 gtgagtgaac cactgtagcc agcctgattt atgctcgccg ccaaacgaca cacggcagcg
    10081 cggtggggac attttcacct cagctattgt gccgcgtttt atcgcgtcac gctgcctgcg
    10141 ggcgttctcc gccgttgtat attcgctgtc atcgtccgat tgggccataa taatcactct
    10201 cgtgttcagc gcctatttcc ggcgcaaacc ctacgaatac ttgctttaat attccgtctg
    10261 acgggtaatc gaacggatcg gcacctacgc caacattttg tttaaatgaa acgcaccaga
    10321 cagaatgcga tgccaccgat ttacccgatt tttgccagtc cccctcctgc gcgtcagtaa
    10381 atacggcggg gcgcgtcatg gggccaaata tacgatcgtt aatccagccc gacacaaata
    10441 atgcggcatt gcgcgctttt tgactgtata agtccgctgc atattcacgc accagatata
    10501 aattacaggt taattcgacc gtcatcgcgg atcccgcgat tacatcattt gatgccgacc
    10561 agctctcaac ctcgaaaaaa attgcaggtg ttatgaatcc gtccggtatc tcggggtata
    10621 tccctaccgt tttaataaat gggatttgca ttaacgcggc gtgaacatta tttgtatatt
    10681 cgtccagcgc gtccataccg ctaatttttc cgctcatagc cgtttacctg ttttggcgtt
    10741 gacatggacg ctaccagcga cgcgtccgcg caaatcctgt tcataatatg ccataaatac
    10801 aggaccaata ttttgatata tataatcatc aatagcatcc tccagcgcct catgcaccgg
    10861 aatcctcatt tcctcaatat tgcggccacc gtttacctgt ttccatatac tgcgatatcc
    10921 aaaacgggtc gcaacaaatg aatcaggcca tcccatagcg gatagtgagg atgatttagg
    10981 cgtaaacgac gctcccgcta ctgttttctt ttttttcaag aattgcccgg tttccggatc
    11041 tcgttcctgt ttcccggttc tcggctggcg cattgtccca cgtaaattat gtattctaaa
    11101 atcatccaga ccataccata atttaacaga actaaggtcc cccgcgccct cttttgaata
    11161 atcgcggcgt ttaataaatt gctgtgtgcg tttattagcg acttttctgc tttttaagcc
    11221 gagggcatca ataatcatac tgactgaaac acggtgcatg tgtttagctg tgcgattcag
    11281 ggcgcggtta tacgccataa tcatctgatg ctctgttgcc tccacggcat cgcgcaaccc
    11341 tgttaatgcg ctcatgtcta tgtcaaacat ttgcgcgtta ctgcgtggat ttgccatttt
    11401 atttatctca aaaaaaaagc gggcaagccc gctaatattt cgaataaacg ctattttcac
    11461 tggttgtttt agggtctaaa taaatatcag tcaatccagt gccatctggt tgcatttccc
    11521 ggatagtcca taatttaccg ataattttaa tggtgtcgcg ttttttcaaa ttaacgcaat
    11581 cgtaatcatg cagggtaatg cacgaatcgc tactggatat acgcccacca gcaatatcaa
    11641 ccccaaaaaa aggggcgtca tatacaccct gcacggttat ttcctgcgga gtatccggaa
    11701 ataacgtaac cccattatca acattaaacg tttcgaccag tgttttatca gcgcggcgca
    11761 tcagttcatt aaatgaactc attagccacc caggcgcacg actgctgttg cgctacctgc
    11821 ggcagcggca gcccacgcaa caccaacgag ggggttactg ctcgatgtgg tggttaattt
    11881 ccctgacgct aaatataatt tcacaccctg cgcccaagaa tccgtggagg tttttggcaa
    11941 ttcaaacacc ccggacatgt gcagcacacc cgttgcgctg gcggcgatat tatcgtgcgc
    12001 aatacccaca accgccccca cgccaaccgc gtcaccacta tttaccgcgt tcgcgccggt
    12061 atttgtccag tcgagggttt tgccatcctc aatataattt tttgccatta tttcacctgt
    12121 tatttatttc gaattacgcg aataaaatta ttcgccctcg cgaccgtcgc cggttgattt
    12181 cagcagtccg cgatagtcca tcggggatac gcccgcatcg atgcgcactt tcattgtcac
    12241 gccgtcaaca gtaaagccgc tggtcgattc aataaccggc gcgtcgttcc cgtcgagata
    12301 cgcaacctcg atcgtgtcgc gcccctgtgc cgctgccaga taccagtttt tctcgcccgc
    12361 gtcgtcaatg cgcggatcag caacaatctg cgccatgccg cgtactgggt tcgaaatgcc
    12421 agagttatta tcagcgccag gaacactcgt ggagttgatg atctgatccg cgagtgactc
    12481 gtttgcggtt gatacgagca aaaacgccgg gcgaatattg agatgccgtt tgccgttttt
    12541 ctgttttttc atcattgagc ggcccgttgc cagcgctgct gctgataacg gcgagtcaat
    12601 gaagtttccg tgatcggtat ggaataacgg tttatcccca aatttcggat tactggacag
    12661 caacgcgtaa accagatcgc cgatcgtggc ctttgccgcc gtccccattg cgtgcgggat
    12721 acgcgtcaac atagacaggt cgtcgttaat gattgcctgg cggtcaatgc tgaatagctc
    12781 gccataggtt gccagcgcga tggttgcgcc gctatcaccc agcgtaacgt atttgtactc
    12841 ggcaccaggg cgaacctcgc gcagcgccgg gaacgagtcg aggccaacgc gattggcggt
    12901 tttaaagtcg gtcagttcgc cttttcgggt ccagacatca aaagtttctt ccgcctcatc
    12961 ccaaccggcc aacgccgatt tatgcgcaac gtccatgaga atattgccga aatcgctgga
    13021 tgaatgagta aacgccatcc cgatcatttc cattcgccct gcaccggaaa taccgatacc
    13081 gcgatcggcc agcgaggcgc gcgccagctc tgccagcgtc atgccgctgt agttattgct
    13141 cccctcgcgc tcctcataac cagcgcggga cataatcacg tttttgattg aatcccccac
    13201 cagattgccg ttacccgcgt aaatatgcgc tccaccagcg ggggcagccc cctgcgcgag
    13261 ggcatccagc aggcgctgac gcgccactgc atcagtgcag gtggtatcgg tcaggcaggt
    13321 gttttgcagg gttgcgaacg ccggaaaatt agtaaaaaca gccgataccg ccgctttacg
    13381 cgcggtttcg cgcgccgcca tcatttcgag tgcctgcgtg gcaatcgcgg cagcgtcctg
    13441 cgcagctggc tgcggtgccg ctggttgagc cgctgccggt tgttgcgttg cctgtggcga
    13501 aggctgcggg gccgccggtt gcggttgtgg ttgcgtgcca gttgcgcgcg gtttgaaaaa
    13561 tttctgtgct gcctgcggca tgtgtgaaaa ctcctttgtt acgttatcgt taatgcaggc
    13621 ggccatttca accttcggca ccagtacatc agcaaacctt ttttccaccg cttccgcgcc
    13681 ggttagccag gtttcggcag acatgagcgt tttaacctcc tccgcatcaa tgcctgtttt
    13741 gttaacatat gcgttaacta tatttttttc gtgccgctca agcagatcgg cgtattcgcg
    13801 cagttcggct gcatcgcccc acgcaccgcc ccacgggttg tgaatcatta cccaggcatt
    13861 ttccgggatg tgcacctgtg cgccgggcaa cattgcgatc acgctcgcca tcgaggccgc
    13921 caggccgtcg atataaatat tcacaacgcc agtgaggcgg cgcagtgtgt taaaaatcgc
    13981 gaacccgtcc agcacgctgc cgcccggtga gtgaatacga atatccaccg ccggtgcgct
    14041 gaataaatca gcgtcggcaa tatcattaat gagtcgccgc gcggaaatac cccacgcgcc
    14101 gatctcttca aaaatcagga tttccgccgg tgcgccgttg gccgccgcgc gtacctcgta
    14161 ccagccgtca cccgccccgg ttctcgccgg ggcgctggct cgcggtgcca gcgtcatttt
    14221 ttgcgtcatt actctgactc ccgttatcgt ttgccggatc tgtgtcgaat accagaccag
    14281 cggttttatt aaatttaatt tcgcggcggc gttgcagttt gaccgccagt ggggaccgcc
    14341 cgcgcgctcg tatccactcg gcctccgtag ctgctccgcc acggatctga gctttccatg
    14401 cctcgctctc tttttgcggg tcaatccagg ggagaaccgg ccccatgtag acggcatcaa
    14461 acagcgattc cggatccacg tcagctggca cttcgacgcc ggataacaaa aacattttca
    14521 gccattcccg gtatacaggg cggactacgc gggagataaa ccagttttgc attactgcgt
    14581 aaccgtctcc cgcctcaatc aattcctggc gctgcgcgga gtaggtgcca ttgtagtcgc
    14641 gagcaattga cgaataaccg gttctcacgc cagcagccag cgcacgcaat tgcccgttgc
    14701 gaaaatccat caaattagga ttcgggcgat cgctgctcaa catttgcagt tcctcgcccg
    14761 gtgccagccg gtcataaatc atgccggggg ccatctcaaa atcccgatag ctgttatctg
    14821 gcgacgtcca gtcggcgtca tcgccgtaca ccgacgcgtc accacgtttg atataaaaac
    14881 ccagcattgc tgatacgcgg gccgcgacgc gctcggcgtc ctcatatgat ttcaaatctc
    14941 ccaggcgcga catgacgccg tgaaataacg acacgccgcg cacctggtgc aggcgtttac
    15001 ggacagccag gtgcagcata ttttctgcgg ggatctggcg cgtatctgtc gagaaccctg
    15061 caccagggtg atttttataa acacagtaac ccacaggaca gccccagcga tttaccgtaa
    15121 taccctgaat aatccggttt gaaccgtcgg caccagacgg ccacataggc acaaaatcgg
    15181 cctcgagtag ctcgagcgcg aattgttccg gcgtgtcgta tttcagatcc gggacagcac
    15241 cgcgcagcaa ctgaataaac acctcgccgt cgcgcaacgc cgaacgcagt aaaattcgct
    15301 cagcctcagc cagcgtaaac attcctgtta cgtccgggtt ttcagcccat ttagcgaacc
    15361 gtgacgccag cagcgctgca aaatcctcgt gcacgtctcc ggccagcgtc agcggctgcg
    15421 gctcaatctg aatgccgtga gggccaacaa tgcgttcctc cagtttatcc agcacaccga
    15481 tcgccaggtc gtggttttca tccagccagc gcgcctgctc tctcagagat acaccagcgg
    15541 caaaaaccgc attgtctgcg ctgcgcgttt cgcgtttcgt cgctcgtgta cgtgacggtt
    15601 tagccgcctc atacgccctg atggcgtcca gttgaatgcg cgcccgcgct cgttttaacg
    15661 acgtaacggg ggcgatcgcg gtggttaatc tgctgaaaat acccattgtt attccctgaa
    15721 tctgacaaga gaaaattgac ggccgccgcc gcgcgtcgcg gtagacaggc gcttatccca
    15781 atactcaaac tctgagcgca gcgcgctgat attttgtcgc gtcagggttc gccccttcat
    15841 gccgaggctc tgcgcctcca gcgcatcctg atacgcggcg aacgcacgat cgcgagcgga
    15901 tttaattttc gaaatttcca taaaacccca ttaaaaaaag ccgctcacgc tggctggcgt
    15961 aaacggcttt cccgttgttt ctgtagtttt taaatcaggc tctggcggtg ccgccggttg
    16021 cgcagcgatt tgaatattcg ctgcctgcgg gaccgc
//
LOCUS       DAHBHC010000103        15764 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHBHC010000103 DAHBHC010000000
VERSION     DAHBHC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.104"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(200..328)
                     /locus_tag="M2A35_004273"
                     /pseudo
     CDS             complement(200..328)
                     /locus_tag="M2A35_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(794..1243)
                     /locus_tag="M2A35_004274"
     CDS             complement(794..1243)
                     /locus_tag="M2A35_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200494.1"
                     /translation="MLLFWCGFFSSIALILSLSQGYNERLYLDEQDRITKENIYVDYL
                     SVNADVLFDICATEQNRCNNIHTTLPEEFAAQLKEKLSNYDNSITPGFCINNGEIIVY
                     TSHNFNIERYKDSSLFNLSDVQSDIKTACSIHDYSGIAVIKMLNKNK"
     gene            complement(1246..1734)
                     /locus_tag="M2A35_004275"
     CDS             complement(1246..1734)
                     /locus_tag="M2A35_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200495.1"
                     /translation="MIFEGRQQKGQSLIEIAMVIILSVLFISGGSYIMTLFKTFSQSC
                     VVATDMNSIRSIIASSTVKYKDKKYDASIITNNNRFNKYQVYFGDDGYTLISGGSPVE
                     IDEFYITCYLAKYNCVWIINQNNNLYAKHMSAVLSPELLSTYGISYSGDSGVTGLLFK
                     RY"
     gene            complement(1724..2272)
                     /locus_tag="M2A35_004276"
     CDS             complement(1724..2272)
                     /locus_tag="M2A35_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCB7200496.1"
                     /translation="MIGKEKGLSLIEVMIGILFFSVISLSVYDHYNRSAEQHYINNIS
                     IVMCRYADALSYYLQEDDKVNNSSVSLSELVSSGYLPSMPEGASVGGYKIKMYVDNKD
                     NGLIALYGDEHYSLTIKSMKPFLGIRGASVEGDKIISTGGYYSLPVSGFTNFSTDNLR
                     GLMLIPSIQTSGQNCDRGGYDF"
     gene            complement(2269..2808)
                     /locus_tag="M2A35_004277"
     CDS             complement(2269..2808)
                     /locus_tag="M2A35_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200497.1"
                     /translation="MFILGNKRKESGLSLIEASLVIILAGLVIVLIQSAISKIIKTAV
                     HENALHDISYVMSNIQDTLQTSCYEKSDDVCEKKITSVTLKFNTKKIKVDIKKTGKYV
                     AVYVLYTLSDNEDFGRYEDFGSMLLTHASAYYGRIDGKGAVNSVLGWWTVNSNSTIFS
                     GGDNNKNLFFYNNFRVNTL"
     gene            complement(2828..3274)
                     /locus_tag="M2A35_004278"
     CDS             complement(2828..3274)
                     /locus_tag="M2A35_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCB7200498.1"
                     /translation="MFSLPLITGIAACVINASSVNNIDPELLLSVMTVEGGTPGSVSV
                     NKNGSQDLGIMQINTHAWLKPISKSFFDGDESSAYNKLKDDACFNISVGAWILRHSIK
                     LEGGNVWEGVGRYHSATPEYKYRYIDKVKNVYGKMTYSQSTHKRNM"
     gene            complement(3256..4005)
                     /locus_tag="M2A35_004279"
     CDS             complement(3256..4005)
                     /locus_tag="M2A35_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCB7200499.1"
                     /translation="MQESILLLLVFIYATTIASFIWLAVERLPHQLKWVDDPVSDLTI
                     WSPGSKCNHCGEKIRWYYLIPVLGYLLCRGKCVYCLSKVPSRYPLTELFCGICCVIIF
                     IFFRDRMYDAVIVSLLFLCLVFLTLIDLRENWLPACVTYPLFWAGMIIPGFASSDDKI
                     IGAFAGFFIMYVSMKLVSAVRKEDVFAGGDVALATAAGAWLGIDKMPLFLILSSVIFI
                     FYSLPARFRGQVFVPMGPALSVSFFICLVYH"
     gene            complement(4012..4968)
                     /gene="bfpF"
                     /locus_tag="M2A35_004280"
     CDS             complement(4012..4968)
                     /gene="bfpF"
                     /locus_tag="M2A35_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus retraction ATPase"
                     /protein_id="HCB7200500.1"
                     /translation="MNIINELNFADLLISKDSYNFRFLEGQPYPICTVPANYNAEVQG
                     MMQELESIREVKGNEFFYLHLGVPYRVAVVQTINGSGFFLRKLKLPVPSLDSLGFSEP
                     FLNTLKLMGKKRGLLLVSGATGSGKSTTIYALLTYYVQKYGDIAISIEDPPEVPVQGN
                     YGNNQEGVWFQMDARELGGYENAMISAMRYNPRYIFLGEIRSAKTAKEAIRAAVNGHL
                     VVSTIHGSSVQGAIYALQQIASAEGEADLVRSIIADGLLGIVHQELRFTERGERSLSA
                     NILCNDGSASISSKIRSGKLELLSSEIEQQRIMLSHGKTLIT"
     gene            complement(4987..6045)
                     /gene="bfpE"
                     /locus_tag="M2A35_004281"
     CDS             complement(4987..6045)
                     /gene="bfpE"
                     /locus_tag="M2A35_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus protein BfpE"
                     /protein_id="HCB7200501.1"
                     /translation="MKEKLNRLLFTSKTRMRVFSKLSRYLSNGVPVTFALAELYKFTS
                     DEGRKKDNPDAFALQRWLIAVRNGKTLAEAMEGWVPSDELSIISAGEISGNVHHALDD
                     IIYMNDTKKKVKGALAGIIYPVVLLLTTCLYLHIFGTQVVPAFSGILPVEKWQGAGRT
                     MYYLAVFVQNYLAITLLSVMMVMLLILATLSRWTGRLRLFFDRFIPWSIYKTIIGCGF
                     LLSLASLINAGIPVPEALRIIMKTASPWYKEKLVAIRYRMLNGDRNLGEALNSSGYIF
                     PSKQMIMDLRSYSALGGFDEMLNKLSVQWQDDSVAYITKQMSVLKNVAIIIMGLVFMW
                     IVSGMFSLQQQISDAARF"
     gene            complement(6052..7659)
                     /gene="tadA"
                     /locus_tag="M2A35_004282"
     CDS             complement(6052..7659)
                     /gene="tadA"
                     /locus_tag="M2A35_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCB7200502.1"
                     /translation="MMLNNTENNSGLTFERFKRNVSEIITGEGGELELTDEQRKYFLI
                     FRNGDFLVSSCHINHHLVQMLREIAARKGYPNLTIYEVNLKDIRLLYEASLRTVQKKT
                     QDLLPVEKRASTLLFECAEMRVSDLHIKVYDTEADIYIRKDGDMELLRQIESHTAHSI
                     LASLYNNADDADATYKINAYQAARIVASKSRLALPPVVQAVRLQFNPLGQGGRYLIAR
                     FLYTDKSEQRKEIDPTRFGFHRLHAESFSRMRALPIGINIISGPTGSGKSTTLKNLLE
                     LLYIEKKKKVNIISIEDPPEYEIEGTAQLPITNVETEAQRGEEYRKAITAALRSDPDI
                     IMPGEARDAEVINLLFTAAMTGHQVWTSLHANNALAIFDRLKDQGVDEFKLTDPELLT
                     GLVAQRLVRRLCDQCSITLTEYTDNGGDLSARDRKIISGHERTVRFPNPRPEKCCGDG
                     YNGRTIIAEVIEPDSKLLRLVSEGKREEAQHYWLTSLGGMALKEHAWLKIVSGEICVM
                     DAVNKTSGIDNISEERKKYLLSRNNEI"
     gene            complement(7666..8160)
                     /locus_tag="M2A35_004283"
     CDS             complement(7666..8160)
                     /locus_tag="M2A35_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200503.1"
                     /translation="MFFGGGLSLKKILFTLLLVVSSVVNAEVEKSVDVSGENDIRKEE
                     KKLPDVDLEQYKDELLSYQQLQRKNMMLKLQAENQKLESEIKKNGSFVSYDTDDNTMH
                     VMSIMTIPKRGLCATIFYGGICRVCKGDVINEDYVVSDITGNYVEIRMVNDISDTGRK
                     IYLR"
     gene            complement(8138..9346)
                     /locus_tag="M2A35_004284"
     CDS             complement(8138..9346)
                     /locus_tag="M2A35_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200504.1"
                     /translation="MIKDNLGVAVIGSKQYAVNLLWGSSQDTETTNQALNKSLALMSS
                     KLYSVIERFQGEQFAVGDKNIGHKRGLVTLLSAIDFDGSSFCGLFPTDNELWLVIGVD
                     KDGMVHFDKSFYSKDDAKKFFFDHVAYRYPWDRTYSPSDVGVGESKSITELSLIKGKK
                     LKEKGAGQLMPVLFFGAGIIICFVIIYNAIDSWLSGKDKDVINEVSPPVISEEVRNVP
                     WGGKSKPASLFSQCIKEMDSFRFLAASVPGWMPEDKVTCKDSNISFSAHRSGGLDIWY
                     EDPSLFFHSGNIPGITKNGNDIITLTWPLDTVRYDSLRLDVNRLNNTQEIIRYLSRQF
                     ENSWINIKFGQPVPDGNTERVDFSFSLNNDPRILLPILSEVNGLVITNIDYDFSGSNW
                     NVSGVFWGGA"
     gene            complement(9343..11001)
                     /gene="bfpB"
                     /locus_tag="M2A35_004285"
     CDS             complement(9343..11001)
                     /gene="bfpB"
                     /locus_tag="M2A35_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bundle-forming pilus secretin BfpB"
                     /protein_id="HCB7200505.1"
                     /translation="MKLGRFSLFLLCPLLASCSGNGFYKDNQGVIDKNILHTDTSLLK
                     SKNKKYYKSSDMVSKTDSIYIGNSSFQTYHGEPLPGKLEGVHGIVLRSSAPLEFDEVL
                     SMIQDASGIPVVKQTTKDVISGGVSSKSLAAAVAEKMNRAAGVNSTDHFDNLMLGVSS
                     NHQIMDVNYQGALSSFLDKVAANYNLYWTYESGRIVFSNEETKRFSISILPGGKYTSK
                     NSISSDSNSSSGSSGSSGSSSSDSGAELKFDSDVDFWKDIENSIKLILGSDGSYSIST
                     STSSVIVRTSSANMKKINEYINTLNAQLERQVTIDVAIYNVTTTDSSNLALSLEALLQ
                     RNGGVLGSVSTSNFAATSGTPSLTGYLNGNGDSSNQVLLNLLAEKGKVSVVTSASVTT
                     MSGQPVPLKVGNDRTYISEIGTVLSQSSTSTTATTSTVTSGFLMNLLPQVADDGNILL
                     QYGVTLSELVGSNDGFDQATVNGTVIQLPNVDSTTFVQSSMLRNGNTLVLAGYEKKRN
                     ESVDQGVGTTSFKLLGGALSGSASRTVTVICITPRIIDLKASGE"
     gene            complement(11008..11409)
                     /locus_tag="M2A35_004286"
     CDS             complement(11008..11409)
                     /locus_tag="M2A35_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HCB7200506.1"
                     /translation="MRILIFFICLLVSNITHAQESTNKGSRLFSGNATSVAPQSSERT
                     VKDDKTDWRVIKGQTLYGVLSDWGQKSGWTVLWNTDYNYTLNASAVFKDMSLDDAVKK
                     LLESMGAVSPKVFIKFYNGNHVIVVTPSPEG"
     gene            complement(11456..12037)
                     /locus_tag="M2A35_004287"
     CDS             complement(11456..12037)
                     /locus_tag="M2A35_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HCB7200507.1"
                     /translation="MMSKIVNKKYEKGLSLIESAMVLALAATVTAGVMFYYQSASDSN
                     KTQNAISEVMSATSAINGLYIGQTSYDGLSASILLNSSAIPDNYKGDDYISNPFGGRL
                     IPAPATAGNNSFGYYVQLDGLDKPACVSLATLNLGTSAKGYGINITNPASTNSFQTTA
                     LSGQAKNSAITPAEAASSCTNENSNNTVTYFMK"
     gene            12460..13200
                     /locus_tag="M2A35_004288"
     CDS             12460..13200
                     /locus_tag="M2A35_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7200508.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     KRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(13479..14456)
                     /locus_tag="M2A35_004289"
     CDS             complement(13479..14456)
                     /locus_tag="M2A35_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCB7200509.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            15548..>15764
                     /locus_tag="M2A35_004290"
     CDS             15548..>15764
                     /locus_tag="M2A35_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F7-2 fimbrial protein"
                     /protein_id="HCB7200510.1"
                     /translation="MNKVIIATMLASTVSFGVNAANQGQGIINFKGTVIDAPCGIAQE
                     SADQTINFGQISKAHLNADGISVKKDLD"
BASE COUNT         4688 a         3551 c         2825 g         4700 t
ORIGIN      
        1 gggagaccgt catccccggt tcaaagctct gctgaacaat tgcgatcttt tcctgtgtgg
       61 tacgccgtct gcgtttctcc ggccctaaga catcaatcat ctgttctcca atgactagtc
      121 taaaaactag tattaagact atcacttatt taagtgatac tggttgtctg gagattcagg
      181 gggccagtct atggacaccc taagcgagat tatgtttttc aaattgttca aggctgagac
      241 cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaatacca
      301 ttgccagcat tatttcccgg ctgcaatacg cttatttatt ggagataact ccgttccata
      361 gaagggattt atcaatacgg ttaaaagtac attccggcag tacattaaaa atatggcgca
      421 gatacgcttc cgagttgata tcgctcagca gacaggttcc gattggtctg cacgattgtg
      481 cataacactc tcagcagtaa tcactgacga agaatgtata gtatttactc ccggaaccga
      541 gagctgaagg ggtctttgcg ataagttatt gtctgtcacc acctgagcat tattgtaata
      601 aaaaacaaaa ccctcatact gacgtcgttt gtgtctgccc gcctaatatc catggcttat
      661 tatacttaat cttctccaga taaagtgact atgctatagc cttatccata atgtatgatc
      721 ggaggtggta tttcccatca gtaatatatc tggcacccaa tattactctg ggaggcttac
      781 agtaccagtg acgctattta tttttattga gcatcttaat aacggcaata ccagaataat
      841 catgtattga acaagctgtt ttaatatcac tctgcacgtc ggaaagatta aacaatgaac
      901 tgtctttgta acgttcaata ttaaaattgt gagatgtata gactataatt tccccattat
      961 ttatacaaaa tccaggtgta atactattat cataattaga taacttctct ttcagttgtg
     1021 ctgcaaactc ctccggcaga gttgtatgta tattattgca tctgttttgt tcagttgcgc
     1081 aaatgtcaaa taaaacatca gcattcactg aaagataatc tacatatata ttttcttttg
     1141 ttattcgatc ttgttcatca agatataacc tttcattata accttgagac aaacttaaaa
     1201 tgagcgcaat tgatgaaaaa aaaccacacc agaataataa cataactagt accgtttaaa
     1261 caaaagccct gtaacaccac tatcgcctga ataactaatg ccatatgtac ttaataactc
     1321 tggtgagaga acagcagaca tatgctttgc atataagtta ttgttttgat taataatcca
     1381 tacacagtta tattttgcta agtagcaggt aatataaaac tcatctatct caactggaga
     1441 acctccagat attaacgtat agccatcatc cccaaaatag acctgatact tattgaaccg
     1501 attgttattt gttataattg atgcatcgta ttttttatct ttatatttaa cagtcgaact
     1561 agctattata cttctgatag agttcatatc tgttgctaca acacaagatt gagaaaaagt
     1621 cttaaaaagg gtcataatat aactaccacc tgaaataaac agaacagaca agataatgac
     1681 cattgctatt tcgataagag actgcccctt ttgctgacgc ccttcaaaaa tcatatccac
     1741 ctctgtcaca attttgtcct gatgtctgaa tagaaggaat aagcattaac cctcttaaat
     1801 tatcagtgct aaaattggta aatccggata caggcaggga ataataacca cctgtagata
     1861 tgatcttatc cccctccaca gatgcaccac gaatccccag aaatggtttc atactcttaa
     1921 tagtaagtga ataatgctca tctccatata acgcaatcag accattatct ttgttatcga
     1981 catacatttt aattttatat ccgccaacac tagctccttc tggcatcgat ggtaaatatc
     2041 cagaagacac cagttcagat aatgatacac tactattgtt tactttgtcg tcttcttgta
     2101 aatagtagct aagcgcatca gcatatctgc acataacgat agagatatta ttaatataat
     2161 gctgttctgc gcttctgttg taatggtcat atacagaaag agatataact gaaaaaaaaa
     2221 ggatgccaat catcacttca atcaatgaaa gacctttttc ttttcctatc ataaagtatt
     2281 cacccggaaa ttattgtaaa agaaaagatt cttgttatta tccccccctg aaaagattgt
     2341 gctgttagaa tttactgtcc accacccaag aacagaattc acagcgcctt taccatcaat
     2401 tcttccataa taagcacttg catgcgtaag taacatgcta ccgaaatcct catatctgcc
     2461 aaaatcttcg ttgtctgata gtgtatacaa aacatacaca gccacatatt ttcccgtttt
     2521 ttttatatct acctttattt ttttcgtgtt aaactttaat gttactgaag ttatcttttt
     2581 ctcacaaaca tcatcacttt tttcataaca tgacgtctgt aatgtatctt gaatattaga
     2641 cattacatat gaaatatcat gcagagcatt ttcatgaaca gcagtcttaa taattttaga
     2701 aattgctgac tgaataagca caataaccag tccagccaaa ataatcacta atgatgcttc
     2761 gattagagac aaacctgact cttttctttt atttccaagt ataaacatag acgaaccatg
     2821 attttcttta catattcctt ttatgtgttg attgactata ggtcattttt ccatagacat
     2881 ttttcacttt atctatatat ctgtatttat actctggtgt ggccgaatga tatcggccta
     2941 caccttccca tacattacca ccctcaagtt taatagaatg gcgaaggatc caggctccta
     3001 ctgatatatt aaaacaggca tcatccttaa gtttattata agcagacgac tcatctccgt
     3061 caaaaaaact cttactgata ggttttaacc atgcatgagt atttatctgc ataataccta
     3121 aatcctgact tccattctta ttaacggaaa cactacctgg agtaccgcct tcaacggtca
     3181 taactgacag taataattca ggatcaatat tattcacaga actggcattg ataacacatg
     3241 ctgctatacc tgtaattaat ggtaaactaa acatataaaa aaagatactg aaagagctgg
     3301 tcccatagga acaaaaacct gacctctgaa tcttgcagga agggagtaaa aaataaatat
     3361 aactgaagac aaaatgagaa ataaaggcat tttatctatt cctaaccatg ctccggcagc
     3421 tgtagcaaga gcaacatccc cgcccgcaaa cacgtcctct tttcttaccg cactaaccaa
     3481 cttcatagag acatacatta taaaaaaacc ggcaaatgca ccgataatct tgtcatcact
     3541 ggatgcaaat ccagggataa tcataccagc ccaaaacaga ggatacgtaa cacaagccgg
     3601 taaccagttc tctcttaaat caatcagagt aagaaaaaca aggcaaagaa aaagaagcga
     3661 cacgattacc gcatcataca tcctgtctct aaaaaatata aatataataa cacagcagat
     3721 gccacagaaa agctctgtta atgggtatcg gctgggaact ttagagagac aataaacaca
     3781 tttcccgcgg cacaataaat agccgagaac aggaataaga taataccatc gaattttctc
     3841 accgcaatga ttacatttac ttcctggtga ccatattgtc aaatcagaaa caggatcgtc
     3901 cacccatttt aactgatgtg gcagacgctc caccgcgagc caaataaaac tggcaattgt
     3961 ggtcgcatat ataaaaacta ataatagtaa tatactctct tgcataatat tttaggtaat
     4021 cagtgttttc ccatggctca acataattcg ttgttgctca atctcactac tcaggagttc
     4081 cagttttcca cttcttattt ttgaagaaat agatgcagag ccatcattac ataaaatgtt
     4141 tgcgctgagt gaccgctctc ctctttctgt aaaacgtaac tcctgatgga ctatccccag
     4201 taatccatca gcaatgatac tacgaacaag atctgcttct ccctcagcag aagctatctg
     4261 ttgtaatgca taaatcgccc cctgaacgga gcttccgtgt attgttgaaa cgacaagatg
     4321 tccattaact gcagcccgaa tggcctcttt cgctgttttg gcgctacgaa tctcccccag
     4381 gaaaatatat cttggattat aacgcatcgc tgaaatcatg gcattttcgt atcccccaag
     4441 ctcacgtgca tccatctgaa accagacgcc ttcctgatta ttaccatagt ttccctgaac
     4501 aggaacttcc ggtggatcct caattgaaat agcaatatca ccgtactttt ggacataata
     4561 tgtcagcaaa gcataaatag ttgttgactt acctgagcct gtagcgccac taaccaaaag
     4621 caatcctctc ttcttcccca tcaattttaa tgtattaagg aatggctcgg aaaatccgag
     4681 gctatcaagt gatggtaccg ggagtttcag ctttcgtaag aaaaaaccag aaccgttaat
     4741 tgtttgcacg accgcaacgc gataaggcac accaagatga agataaaaaa actcatttcc
     4801 ctttacttcc cggatagact cgagctcttg catcattccc tgaacttcag cattataatt
     4861 agcaggaaca gtgcatattg gatatggttg cccttcgaga aaacggaagt tatatgaatc
     4921 ctttgagatt agtaaatccg caaagttgag ttcattaata atgttcatta tgctacacca
     4981 aaaaaatcag aatcttgccg catcgctaat ttgttgttgt aatgaaaaca tacctgatac
     5041 tatccacata aaaacaagcc ccataataat gatagcaaca ttttttaaaa cagacatttg
     5101 tttcgttata taggcaacac tgtcatcctg ccattgaact gaaagtttat taagcatttc
     5161 atcaaaaccg ccaagcgcag agtaagaacg gaggtccatt atcatctgct ttgatggaaa
     5221 aatataacca gatgaattaa gggcttcacc aagatttcta tctccattta acatcctgta
     5281 tcttatagca actaattttt ccttatacca aggacttgct gttttcatta ttattcgtaa
     5341 ggcttccggt acaggaatac ctgcatttat aagcgatgct agtgataaca ggaaaccaca
     5401 tcctataatg gttttgtata tcgaccaggg aataaatctg tcaaagaaga gacgcaatcg
     5461 tccagtccat cttgatagtg ttgccagtat taataacata accatcataa cagacaacaa
     5521 tgtaatagca agataatttt gaacgaatac agcaagatag tacatagtcc tgcctgctcc
     5581 ctgccatttc tctacgggca ggatgcctga aaaagccgga acaacctgag tcccaaagat
     5641 atgcaaatac agacacgttg tcagaagcag gactactgga taaataattc ctgccagtgc
     5701 accctttact ttcttttttg tatcattcat ataaatgata tcatccaacg catggtgaac
     5761 attacctgag atttccccgg cggaaattat tgacagttca tcagaaggaa cccacccctc
     5821 catcgcttca gctaatgttt tcccatttct tacagcaatt aaccatctct gtagagcaaa
     5881 agcgtccgga ttatcttttt ttcttccctc atcagacgta aacttataaa gctccgccag
     5941 agcaaacgtt actgggacac cattgcttag atatctggat aatttgctaa aaacacgcat
     6001 tctcgttttc gacgtaaata gcaacctgtt taatttctct ttcatagaaa actatatctc
     6061 attatttctg ctgagaagat atttttttct ctcttctgaa atattgtcga ttcctgaagt
     6121 tttatttact gcatccataa cacatatttc accagaaact atttttaacc atgcatgttc
     6181 ttttaaggcc ataccaccaa gagatgttaa ccaatagtgt tgagcttcct cacgctttcc
     6241 ttcagaaacc aacctcagta attttgagtc aggttcaata acttcagcga tgattgtcct
     6301 tccattataa ccatctccac aacatttttc aggtcgagga ttggggaatc gtacagttct
     6361 ttcatgccct gaaattattt ttctgtccct ggcagataga tctccaccat tgtctgtata
     6421 ttctgttaat gtaatactac actggtcgca taatctccgg actaatcgct gggcaaccaa
     6481 tccagtgagc aattcaggat ctgtaagttt aaattcatcc acaccctgat cttttaatcg
     6541 atcaaatatg gccagtgcat tatttgcatg taatgatgtc cacacctgat gccctgtcat
     6601 tgctgcagtg aaaagaagat ttattacttc agcatccctg gcctcccctg gcattattat
     6661 atccggatct gaacgcaaag cggctgtaat ggctttacga tactcttcgc ccctttgcgc
     6721 ctctgtttca acgtttgtta taggtaactg agccgtacct tcaatttcat actcaggagg
     6781 atcttcaata ctgattatgt tgactttttt ctttttttca atatacaaca actcaagtaa
     6841 atttttaagg gttgttgatt tacccgaacc agtcggcccg gagataatat ttataccaat
     6901 tgggagtgcc ctcattcttg aaaatgattc agcatgtaaa cgatgaaacc cgaatctggt
     6961 tggatctatt tcttttcgtt gttcactttt atccgtatac aagaatcgtg cgattagata
     7021 tcgcccaccc tgaccaagag ggttaaactg caggcgaaca gcctgaacta caggaggtaa
     7081 cgccagtctc gattttgaag caactatgcg agcagcctgg taagcattaa ttttataggt
     7141 tgcatctgca tcatctgcgt tattatataa tgatgcaagg atagaatgcg ccgtatgaga
     7201 ctcaatctga cgcaagagtt ccatatctcc atctttacgt atataaatat cagcctcagt
     7261 atcatacact ttaatatgta aatcacttac tctcatctcg gcacattcaa aaagtagcgt
     7321 tgaagcacgc ttctctactg gcaataaatc ctgtgtcttt ttttgtacgg ttctgagaga
     7381 tgcttcatac aataatctga tatcttttaa gttcacctca taaattgtga gatttggata
     7441 cccttttctt gctgcaatct cacgtaacat ctgaaccaga tgatgattaa tgtggcatga
     7501 agagacaaga aaatcaccat tcctgaatat cagaaagtat tttctctgtt catcagtaag
     7561 ctcaagctcc cccccttctc ccgtgatgat ttcagataca ttacgcttaa atcgttcaaa
     7621 ggtcaggcct gaattatttt ctgtgttgtt taacatcaaa ctaacctacc tcaaataaat
     7681 tttccttccg gtgtcagata tgtcattaac catcctgatc tcaacgtaat tcccagtaat
     7741 gtcactcacc acataatctt cattaataac atcaccttta cagactctgc atatgccccc
     7801 ataaaatatg gttgcacata atcctctttt aggaattgtc attatagaca ttacatgcat
     7861 ggtgttatca tctgtatcat atgagacaaa tgatccattt ttttttattt cactctccag
     7921 cttttgattt tcagcctgta atttaagcat catattctta cgctgaagct gctggtaaga
     7981 aagcaactca tctttatact gttccaaatc aacatcaggc aatttttttt cttcctttct
     8041 gatatcattc tcaccagaaa catcaacact cttctctacc tcagcattca cgactgaaga
     8101 aacaacaagc aaaagagtaa acaatatttt tttcaaacta agcccccccc caaaaaacac
     8161 cactcacatt ccagttactt cctgaaaaat cataatcaat attagtaata accagtccat
     8221 ttacttcaga aagaattgga agcaagattc ttgggtcatt gtttaatgaa aaagaaaaat
     8281 ccactcgttc tgtgttaccg tctggtacag gctgtccaaa ttttatatta atccagctat
     8341 tctcaaactg acgtgaaagg tatcttatta tttcctgagt attattcaat ctgtttacgt
     8401 caagtctcaa actgtcatat ctaaccgtat ccagaggcca tgtcaatgtt attatatcat
     8461 taccattttt tgtgatacct ggtatatttc ccgagtgaaa aaacaaggat ggatcttcat
     8521 accatatatc cagcccgcca gaacgatgag cactgaagct tatattagag tccttacagg
     8581 tcactttatc ttcaggcatc caaccaggaa cagatgctgc gaggaaacgg aatgaatcca
     8641 tttcttttat acattgtgaa aacaatgaag ctggtttgct tttccctccc cacgggacat
     8701 tcctgacttc ctcactgatt actggaggtg acacttcgtt aataacatcc ttatctttac
     8761 cacttaacca tgagtctata gcattatata tgatgacgaa gcatattata attccagcgc
     8821 caaagaaaag cactggcatt agttgaccag cacctttttc ctttaatttt ttccctttaa
     8881 taagggaaag ctcggtaata ctcttgcttt cacctactcc aacatctgaa gggctgtatg
     8941 tcctgtccca cgggtatcta tatgctacat gatcgaaaaa aaatttttta gcatcatcct
     9001 tactgtaaaa tgacttgtca aagtgaacca ttccatcttt atctacacca ataacaagcc
     9061 acaactcatt atcggtagga aataacccac aaaaagaact accatcaaaa tcaattgcag
     9121 acagtagggt gaccaggcct cttttgtgac caatattttt atctcctaca gcaaactgtt
     9181 caccctgaaa tcgttcaata acggaatata gctttgagga catcaacgcc aggctcttat
     9241 tcagagcctg gtttgttgtt tctgtatcct gagagcttcc ccacaataaa ttaacggcat
     9301 attgtttcga accaattacc gctacgccca gattatcctt tatcattcgc cagaagcctt
     9361 gagatcaata attctcgggg ttatacaaat gacagtgact gttcgggatg cacttccact
     9421 cagtgcgcca cctaagagtt taaaactggt tgtcccaaca ccctgatcca cactttcgtt
     9481 tcttttcttc tcatatcctg ccagaacaag agtattacca ttgcgaagca tgctcgactg
     9541 cacaaaggtt gtagaatcaa cattaggtaa ctgaatcact gttccattca cagttgcctg
     9601 atcgaaacca tcatttgatc caactagttc tgaaagtgtt acaccatact gcaacagtat
     9661 atttccgtca tcagcaacct gcggtagcaa attcatcaga aaaccgctgg tgacagttga
     9721 tgtagtggct gttgtactgg ttgatgactg gcttaacacc gtccctatct cagatatata
     9781 tgttctgtca tttcctactt tcaaaggcac cggctgacct gacatcgtcg ttactgatgc
     9841 cgatgtcacc acgctgactt tccctttttc ggctagtaag ttaagtaaaa cctggttact
     9901 tgagtcgcca tttccattca gatatcccgt tagtgaaggt gttccgcttg ttgccgcaaa
     9961 attggaggtc gaaacagagc ccagaacacc accgttacgc tgtagcaaag cttccagaga
    10021 caatgccaga ttactgctgt cagttgttgt aacattatag atcgcaacat caatggtaac
    10081 ctgtctttca agttgtgcgt tcagtgtatt aatgtactcg tttatcttct tcatgtttgc
    10141 agaagatgtc ctgacaataa cagacgatgt acttgtcgat attgaatatg agccgtcgct
    10201 acctaatatc aactttatcg aattctcaat atctttccag aaatccacat ctgagtcgaa
    10261 tttcaactct gccccggaat cactggaact acttcccgaa cttcccgaac ttcccgaact
    10321 gctattgctg tccgaactga tagagttttt tgatgtatat ttaccacctg gtaatatact
    10381 tatactgaat cgtttagttt cctcgtttga aaaaacgatc ctgccagact catatgtcca
    10441 gtacaggtta tagtttgccg caactttatc aagaaacgaa gaaagagcac cctgataatt
    10501 aacatccata atctgatggt ttgatgatac cccaagcatc agattatcaa aatggtcagt
    10561 actattcacc cccgcggcac ggttcatttt ttccgcaaca gcagccgcca gacttttgct
    10621 ggacacccct ccactgatca catctttcgt ggtctgtttt acaacaggaa taccactggc
    10681 atcctgaatc atagagagca cttcatcgaa ttccagagga gcacttgacc tcaaaacaat
    10741 tccgtgtaca ccttctaatt tgcctggcag gggttcacca tgatatgtct ggaaactact
    10801 gtttccaata taaatactgt ccgttttgct taccatatca ctgcttttat aatatttttt
    10861 attcttcgac ttcagcaatg aggtgtcagt atgcaaaatg tttttatcga ttaccccctg
    10921 attgtcttta taaaaaccat tacccgagca agatgccaga agcggacaca ataagaaaag
    10981 tgaaaacctg cctagtttca tttttaatta tccttcgggc gatggtgtca cgacaatgac
    11041 atgattcccg ttataaaatt taataaagac ctttggagac acggctccca tcgattctaa
    11101 taatttcttt accgcatcat ccagcgacat atccttaaag actgcagatg catttaaagt
    11161 atagttataa tcggtattcc atagcactgt ccatccactc ttttgccccc agtcagaaag
    11221 cacgccatat aaagtctgtc cttttattac acgccagtca gtcttatcat ccttaactgt
    11281 tctttcactt gactgtggtg cgactgacgt tgcatttcct gaaaaaagcc tgctgccttt
    11341 atttgtactt tcctgagcat gagtgatatt ggataccaaa agacaaataa aaaagattag
    11401 aatcctcaca ttcacctccc atgaagcacg cacaatacag tgcgtgctgt gtaaattact
    11461 tcatgaaata tgtaactgta ttattactat tttcattggt acaggagctc gctgcttctg
    11521 caggagtaat agcactgttt tttgcctggc cgcttaatgc cgtcgtctgg aaagaattag
    11581 tgctagctgg gttggtaata tttataccgt agcctttagc cgatgttcct aagtttagag
    11641 ttgccagact gacacatgca ggtttatcca atccatcgag ttgtacataa taaccaaatg
    11701 agttattacc tgcagtagct ggagccggaa taagtctacc tccaaacggg ttagaaatat
    11761 aatcatctcc cttgtagtta tctggaattg cagacgaatt aagtaaaatg cttgcgctta
    11821 atccatcata agatgtctgc ccaatataca aaccattaat tgcagaagta gcactcatga
    11881 cttcggaaat agcattctgt gttttattgg aatcagatgc agactgatag taaaacatca
    11941 caccagcggt aacggttgca gcaagtgcca gtaccattgc cgattcaatc aaagacagac
    12001 ctttttcata ttttttattc acgattttag acatcataaa actgttttcc ttaagaatcc
    12061 ccttgtttat aggtataagt atattcttaa tatctcccct cctctcgcaa gaaaaaaaaa
    12121 actttttact ctttttttct ttttttttta tactccctca cacaaaaatg tcctgtatat
    12181 aaactgtcaa tcccaccatg aattagtttc cggcgctcct ggcattaccc catttctccc
    12241 aaaaacggca aaccagctct cagcccgcgc catgactagc ttaagacagg atccctgcgg
    12301 caaaacgcgc ctttacgcat ttggttttac ccgcaataat gcgtgataag caaacaaaag
    12361 gatccgcgcg ctgcgattta ttgtcgtgga aggatccggg cgttcaggtc agaataactg
    12421 tatccataaa cagtgcgtgt gctacgtgaa aaataactca tgaacaatgt cattcccctg
    12481 cagaattcac cagaacgcgt ctccctgtta cccattgcgc caggggtgga ttttgcaaca
    12541 gcgctctccc tcagaagaat ggccacttcc acgggggcca caccggccta cctgctggcc
    12601 ccggaagtga gtgcccttct tttctatatg ccggatcagc gtcaccatat gctgttcgcc
    12661 actctctgga ataccggaat gcgtattggc gaagcccgga tgctgacgcc ggaatcattt
    12721 gacctggatg gtgtaagacc gtttgtgcgg atcctgtccg aaaaagtgcg tgcacgacgc
    12781 ggacgcccgc caaaagatga agtccgcctg gtcccgctga cagatataag ctatgtcagg
    12841 cagatggaga gctggatgat caccacccgg ccccggcgcc gtgaaccgtt atgggccgtg
    12901 acggatgaaa cgatgcggaa ctggctgaaa caggcagtga agcgggccga agctgacggg
    12961 gtacactttt cgattccggt cacaccacac accttccggc acagctatat catgcacatg
    13021 ctctatcacc gccagccccg gaaagtcatc caggcactgg ctggtcacag ggatccacgt
    13081 tcgatggagg tttataccag agtgtttgca ctggatatgg ctgccacgct ggcagtgcct
    13141 ttcacaggtg acgggcggga tgctgcggag atcctgcgta cactgcctcc cctgaagtaa
    13201 cggcacttcc ttatataagc agtggtcagc agaacagaca taacataagc tggagcaggt
    13261 agataagctg tagtgagtaa accatgtttt atgaagggag caatgcctca gcatcaggtt
    13321 acggggtgac tcacgtaagg gacaggcaga tggcagctca gccacaggca acactgcagg
    13381 aaactgaata taaactgcag tgagcagacc actcactgca cctgataata taagctgtag
    13441 tcagtaaagg agcactcttc actgactaca gtttatgttc agcgggattt gaagagtttt
    13501 tccaggtcat ccagcgtgat gcccagtttc tcaagcaagg caagcttctc cgccatctcc
    13561 ggactgactt tttctgcagg tgaaggtggc aacggatttt ccttactctc atcattcggc
    13621 gcttttaacc ggggacgccg gtagtgaata cagaagagtt ttgttcgccc ccgctggatc
    13681 tccgtgtaat caagatatcc aatctcgcgc aactgctcca ttgcccgtct gaccgtctgg
    13741 ttctgggaaa ataccggaga cttcagattg aggcgtgcac gcagccgcgc cagcgatacc
    13801 ggtgccggat cccggggcag gctctctata aaggtgtaga gtgcctgggc ggactcccgt
    13861 cgcttcaggg cattaatcgc cttaagctgg agaaggactt ttctgtcaaa ctggtacagt
    13921 tcaaacaggc ggggatcggc ctgtaactga acaatatccc gctcagtatc gtagtaggct
    13981 gactgtacca gatgggtgat gtattcccgg gtgtgcttct catcggtacg ggaaaacgag
    14041 atcacggtac cggcaattcg cttcagggaa gggctgatgc gctcacgcag cctgcgggat
    14101 gactggcttg aaggtatacc acacagtttt gcaaactcaa caaaaggcag ttcaactttg
    14161 tcaccagtca cgttatggcg ggcaaaggaa tgaatgatcc ccacccaggt cttgaaatcg
    14221 ttatccatgt ccagacgggg gccggtgatc tcaaccttat cgaatccctc agcccgggcc
    14281 agggaaagac gcgtcagttc ttccgtggca tcggtgcgtg acagtgtatt ttttttactg
    14341 ttcttcagtg acttaagggt cggtacaaag acgcccagac gcatcagcgc cacaggttgt
    14401 acggtgttgt tattgtttgg tgtcagtgtc accagctcac cggacgactt atccacctcg
    14461 ccgaacagtt ttttgatgtc tgaattttcg ttttccagaa agcctcctgc ctgtggacag
    14521 taaaagaata aacagaaaat cgtgtgtcag aaccccgtca actgactgaa gaaaaacctt
    14581 gcagtctgta tttaacaact gagtctggcg tactgaccat agtttatgtc attaacttac
    14641 ttcagtatat ggattactga atacagctta tcattagcat gttacagttt atcagtaaca
    14701 tgccacagct tatgtgatcg tggtcccggc ccatgagtga caaggtttgc agcgatatgg
    14761 ggatctttaa tggatccttt tttgatcatt catggatctg tttactggat cgaacttctg
    14821 aatatgtgga taaatcatcc cagggaaaca gcatacacaa ccgcttataa gggctttagg
    14881 gcgtatctgc tcactgtaac tacagataca ttgcgcttcc tcgccggttc gcgtgcttcg
    14941 cccggtcacc ggtctgcggc gagcaccgtt ttttttgatt gctataccgg agcagaaatc
    15001 tgatagccaa tccgcaggta acacactgta attgctgtat tttctgtaca atatctgttt
    15061 cactgtgcac cctgctggct cccaaaacac aatggcgcag gaacagaaaa taaagaaata
    15121 ataaaacaaa ttttatacaa aaatatattt tgcatttttt ttggtctttt attgcagttt
    15181 ttgacacaaa acagaagatt gttatatttt ttattctgag aataactatt gttatcagtg
    15241 ttttattcgt aataaatcga aaagcataga aactatatct ttgttataat ctgtttctgg
    15301 ttatttattc tgaaaaagta gattgtgatg ataaatcatt attattgtga tttttttaat
    15361 tctaaataaa attaaccaaa aaaaataaga aaagctgaat tttgatgtga agcagaatat
    15421 acgaacctct taacatgtga taattaatta ttcacattct tttatgagac ataaaagtac
    15481 tttttgttga gtgacagaat ttttgccact caataaaatg tattttttca actcagggtt
    15541 ttgttatatg aataaagtta ttattgcaac catgcttgct tctactgttt catttggcgt
    15601 taatgctgct aatcagggac agggcattat taacttcaaa ggtacagtaa ttgatgctcc
    15661 ttgtggtatt gcacaagaat cagcagatca gacaattaat ttcggtcaga tttctaaagc
    15721 acatttgaat gcagacggaa tttccgtaaa aaaagatctg gata
//
LOCUS       DAHBHC010000104        15018 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHBHC010000104 DAHBHC010000000
VERSION     DAHBHC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.105"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..691
                     /locus_tag="M2A35_004291"
     CDS             <1..691
                     /locus_tag="M2A35_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCB7200511.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     QIARLAEQSTD"
     gene            790..1803
                     /locus_tag="M2A35_004292"
     CDS             790..1803
                     /locus_tag="M2A35_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCB7200512.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTATWLRDHGK
                     LPR"
     gene            complement(1800..2630)
                     /gene="amiD"
                     /locus_tag="M2A35_004293"
     CDS             complement(1800..2630)
                     /gene="amiD"
                     /locus_tag="M2A35_004293"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCB7200513.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2627..2950)
                     /locus_tag="M2A35_004294"
     CDS             complement(2627..2950)
                     /locus_tag="M2A35_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCB7200514.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3076..3591
                     /locus_tag="M2A35_004295"
     CDS             3076..3591
                     /locus_tag="M2A35_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCB7200515.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHHSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3809..4537
                     /gene="artP"
                     /locus_tag="M2A35_004296"
     CDS             3809..4537
                     /gene="artP"
                     /locus_tag="M2A35_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCB7200516.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4555..5286
                     /gene="artJ"
                     /locus_tag="M2A35_004297"
     CDS             4555..5286
                     /gene="artJ"
                     /locus_tag="M2A35_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCB7200517.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5293..6009
                     /gene="artQ"
                     /locus_tag="M2A35_004298"
     CDS             5293..6009
                     /gene="artQ"
                     /locus_tag="M2A35_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCB7200518.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6009..6677
                     /gene="artM"
                     /locus_tag="M2A35_004299"
     CDS             6009..6677
                     /gene="artM"
                     /locus_tag="M2A35_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCB7200519.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6969..7700
                     /gene="artJ"
                     /locus_tag="M2A35_004300"
     CDS             6969..7700
                     /gene="artJ"
                     /locus_tag="M2A35_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCB7200520.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7875..9002)
                     /gene="rlmC"
                     /locus_tag="M2A35_004301"
     CDS             complement(7875..9002)
                     /gene="rlmC"
                     /locus_tag="M2A35_004301"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCB7200521.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(9043..9531)
                     /locus_tag="M2A35_004302"
     CDS             complement(9043..9531)
                     /locus_tag="M2A35_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCB7200522.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9591..10436)
                     /gene="potI"
                     /locus_tag="M2A35_004303"
     CDS             complement(9591..10436)
                     /gene="potI"
                     /locus_tag="M2A35_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCB7200523.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALAALILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(10433..11386)
                     /gene="potH"
                     /locus_tag="M2A35_004304"
     CDS             complement(10433..11386)
                     /gene="potH"
                     /locus_tag="M2A35_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCB7200524.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11396..12529)
                     /gene="potG"
                     /locus_tag="M2A35_004305"
     CDS             complement(11396..12529)
                     /gene="potG"
                     /locus_tag="M2A35_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCB7200525.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFLSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGDQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12624..13736)
                     /gene="potF"
                     /locus_tag="M2A35_004306"
     CDS             complement(12624..13736)
                     /gene="potF"
                     /locus_tag="M2A35_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCB7200526.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14088..14564)
                     /locus_tag="M2A35_004307"
     CDS             complement(14088..14564)
                     /locus_tag="M2A35_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCB7200527.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14652..>15018)
                     /locus_tag="M2A35_004308"
     CDS             complement(14652..>15018)
                     /locus_tag="M2A35_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HCB7200528.1"
                     /translation="IKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGGAASVA
                     SITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGIDIAGK
                     MIRWIERHATTEYCLKTGG"
BASE COUNT         3726 a         4023 c         3760 g         3509 t
ORIGIN      
        1 caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg ggctgaaaca
       61 cgatctgctg gcggatgata ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt
      121 cgatgacgcg gtacgtagca cgttgaaaga ggaggaaaaa ctggtcaact ccagcgactg
      181 gggctacgac gctcaggcct ttgcccgctg gcgaccggag tacggttatt ttgccaaaca
      241 ggcggggttt accgttaaaa cgtccgccag ccttgctgct ttatggcagg tagtgaacca
      301 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
      361 ggaccgcgcg atcggtcata agctggcgaa agggcgcccg gagcgcgaat atttacagac
      421 tggcgatgcg gtagatagct ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt
      481 gttatttggc atgaaagcgc cggggctggg acgactgtgt tttagcctgg aagataaagg
      541 cgactatcgt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
      601 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacaaat
      661 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
      721 ttcccattgc atcatggggg aaatcacgga agaatgcgca cgttattttc tcaacaacag
      781 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
      841 gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggc
      901 aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga gctggtttca
      961 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
     1021 tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg
     1081 ggtgaatggg ctgtcgcctg gggtgtgcgt aactttattc atatctcttc cccctccctg
     1141 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
     1201 aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct ttcgcaggcg
     1261 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
     1321 gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct gttaccgcat
     1381 ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc aatgtggctg
     1441 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat caccaacggc
     1501 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
     1561 cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat ggagcgttta
     1621 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa gttgaatttt
     1681 gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc ggtcatcacc
     1741 ctggatgaag gtatcgagaa aactgccacc tggctacgcg accacggaaa actgccgcgc
     1801 taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc tgcatccgct
     1861 tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac gcgccgctgc
     1921 tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag ctccagcaat
     1981 gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt aacccgctgc
     2041 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
     2101 ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg
     2161 tgataacggg caataatatc tttcgccagt ggaataagcg cctgaatctg tgccggttca
     2221 aacggggcaa aatatttcac tccggccgat ttttgccagc cacggttttc cagctcaatg
     2281 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
     2341 gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta tcgcggtggt
     2401 accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta
     2461 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
     2521 gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgca
     2581 caccctgcca gtaacagagc ggcagcgatc agccagaaga ctcttctcat cgacgcgttt
     2641 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
     2701 agtcgatatc aataccgacg acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt
     2761 cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg gaacgtccgc
     2821 caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct aaaatcgctt
     2881 cgccggtcac cacaccgcaa tattcaacga tggtctggcc ttccagagtt ggggttgtag
     2941 aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc attatcagac
     3001 tgatacgcta ttattgaaag gatatcatta ttatcgtaga acgctttcag agcgatcgct
     3061 tataaggaaa tcattatgcg ctacagcaaa ttgacaatgc ttatcccctg cgcactgctc
     3121 ctcagtgcct gcaccacagt cactccagct tataaagata acggcacacg cagtggtcct
     3181 tgcgttgaag gaggcccgga taacgtggcc cagcaatttt atgactaccg cattttgcac
     3241 cacagcaacg acatcaccgc cttgcgccct tatctgagcg acaaactggc gacactgctt
     3301 agcgatgcct cccgggataa taaccatcgg gaactactga ccaacgatcc attctccagt
     3361 cgcacaacct tgccagagag cgcccgtgtt gccagtgcct caacaatccc gaatcgtgat
     3421 gcgcgaaata ttcctctgcg tgtggatcta aaacagggtg atcagagctg gcaggatgaa
     3481 gtgttaatga ttcaggaagg ccagtgttgg gtgatcgacg atgtacgcta tttaggcggc
     3541 agcgttcatg ccacagcggg tacgctccgt cagtccatcg agaatcgcta acgccttgat
     3601 caaccaataa accccgtaaa ttgtccggca tttcgtgagg aatgccgaca tttatgctca
     3661 ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt tgcttttaac
     3721 ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg acctcaatag
     3781 ctatcagact gccagtatac gagtgtcaat gagtattcaa ttaaacggca ttaattgctt
     3841 ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg gcgaaacgct
     3901 ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct
     3961 tgagatgccg cgctccggta cgctcaacat tgcaggcaac catttcgatt tcaccaaaac
     4021 accctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata
     4081 caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct gccgtgtact
     4141 ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct
     4201 caaaccttat agcgatcgtt acccgctgca tctttctggt ggtcagcagc agcgtgttgc
     4261 tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc
     4321 actggacccg gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa
     4381 tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt
     4441 ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac gcgagctgct ttaccgagcc
     4501 gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac aacaatgaaa
     4561 aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc tgccgaaacc
     4621 attcgttttg ctaccgaagc ctcctatcct ccgtttgaat cgattgatgc aaacaaccag
     4681 atcgttggtt ttgacgtcga cctggcacag gcactgtgta aagagattga tgcaacctgt
     4741 actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa
     4801 gcagtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtact gtttaccacc
     4861 ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat
     4921 cagctgaaag gcaaaaaagt cggcgttcag aacgggacga cacaccagaa attcattatg
     4981 gataagcacc cggaaatcac taccgttccg tatgacagct accagaacgc aaaactggat
     5041 ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg
     5101 aaagataacc cgaaactggc ggcggtgggc gacaaagtga ccgataaaga ttacttcggc
     5161 actggcctcg gcatcgcggt acgtcagggc aacactgagc tgcagcagaa actcaacact
     5221 gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa atggttccag
     5281 aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat gaccgtcggc
     5341 cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc ggtatgggag
     5401 tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc
     5461 ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct gctgctgacg
     5521 ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccagtgca gatggacatt
     5581 gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc
     5641 gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag
     5701 tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt gatgccgcag
     5761 atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc tggtgctgct gaaagatacc
     5821 gcgctggtca gtttgattag tgtgaatgat ttaatgctgc aaacaaaaag catcgctact
     5881 cgtacccagg aaccatttac ctggtacatt gtggcggcgg cgatttacct ggtgatcacc
     5941 ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt tgagcggagg
     6001 cccagctaat gtttgagtat ttacccgaac tgatgaaagg gctacacacc agcctgacgc
     6061 taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc atcatcctga
     6121 cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta tatcacgctg tttaccggta
     6181 cgccgctgct ggtgcagatc ttcctgattt attacgggcc gggccagttt ccgactttgc
     6241 aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt
     6301 tatcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt cgtgcgatcc
     6361 cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat acgctggcga
     6421 tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg
     6481 tgttcaaaag tacctctctg gcttacacca ttacgctgat ggaagtgatg ggatacagcc
     6541 agttgttgta cggacgcacc tacgatgtaa tggtgttcgg tgcggcaggg attatttacc
     6601 tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat cgagcgcaaa gcactggcat
     6661 tcgaacggcg aaattaatgt agctgcgtac attcgccggg ggggtttgta ggtctgataa
     6721 gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg gcgtggacgc
     6781 cttatccggc ctacatagtg cataaattct tataatgaag acggacaact cactaaggta
     6841 tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat aaattcactt
     6901 gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg acagacggga
     6961 gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact ttcggtgctt
     7021 ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt gaatctatag
     7081 gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg tgcaaacaaa
     7141 tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg tcactgaaat
     7201 tcagaaaata tgacgccgta atctccggta tggatatcac gcccgagcgt agcaagcagg
     7261 tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa aaagatacct
     7321 acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc accacgcacc
     7381 agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac agttatcaga
     7441 atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac acagcggtgg
     7501 taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc
     7561 cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg
     7621 aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag aaaatcaatg
     7681 accagtggtt tccgcagtaa ttgagactga tgacaaaagt aagattgcct gatgcgctgc
     7741 gcttatccgg cccacatcaa ctctgcaatg tattgattta gcatgatttt gtaggccgga
     7801 taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg
     7861 cacctgcggc ttttttattg cttcaccagc agcgtcagca cttcatagtg cgcggtgtgc
     7921 gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc gcggatatct
     7981 ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc cattgttgag
     8041 agataatcac atagcggttt accaatgccg cggcgcggcg gattaaccag caccagctcc
     8101 ggcacctccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc
     8161 gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga tgcaatttcg
     8221 atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag
     8281 aacagatccc acatatgttt aaccggcagc tgccgtaccc agtcgcgcgc ggtagcgtac
     8341 agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat ccacagcggt
     8401 acgtcattaa aacgctctgc cagcgcctgt tgttcagtaa ggtagatctc cgtctcccct
     8461 tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag ttgcggtagt
     8521 tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc agaacgcagt
     8581 acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat gtatttcagt
     8641 tcgccacgct tacgcgccac gttgtagggt gtcagccccg cacgggcgat aaacggtttt
     8701 agcgccgcaa aaacgggcgc aaatgaggca ggataaagcg ggcagtcaca aaggtcttct
     8761 ggtgtgccgt cgcgatgcag cataccgagc agtggttttt caacgctacc actcaccacc
     8821 attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca ccattcctca
     8881 accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag ttgctctgga
     8941 atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc
     9001 atacattgac cttcacatca tcaggggcga cgattataca cattattgca actggaagaa
     9061 ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg gcagcttctg
     9121 cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga acagctccgg
     9181 ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag
     9241 atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc agatctcaat
     9301 gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg
     9361 atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag
     9421 gccacggatc ataataatgg cgagcgccgc cacctgcacc agcgcaggca cattcaggcg
     9481 agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat
     9541 gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct tcagccatgt
     9601 cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag ataccaggcg
     9661 ataaatccga caattccgac cgcaccgaga attaacgctg caagggcgtt gatttccgga
     9721 ttcaccccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc tcccggcccg
     9781 gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag taaccagcca
     9841 gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt cagcggcgtc
     9901 gcaccgagat ccatcgctgc ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa
     9961 ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata
    10021 ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa caacagcgac
    10081 aagcccgtga tgacatctgg catcaccagc ggcgcggtga tcataaaggc aaagccattt
    10141 gatccgcgaa acctgccaaa gcgcaccagc accaccgccg caatagtccc gaggatcgcc
    10201 gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact catcatcgcg
    10261 tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac cgtcaccagc
    10321 ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag aaaggtaaag
    10381 cccagcagca aaatcacaat ccgccagggt gaacgaacta ccggtaaatt attcatccgt
    10441 gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca attagcagca
    10501 gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg aaaaactctt
    10561 gccatagcac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc gggatcacaa
    10621 actcgcccac agccgggata aacaccagca tcgatccggc aataatccca cctttagtca
    10681 gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc agcgctgctt
    10741 ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc ataaacggca
    10801 cgtaagcgta aacaatgccg atataaacgg ccagattggt atgcagaatg gtcagcggct
    10861 ggtctataac ccccagccac agcagaaaat tattcagcac accgttgttt tttaatattc
    10921 ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc accagtagta
    10981 ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc ggatagccga
    11041 tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga taagcatcga
    11101 aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg atggacagtt
    11161 gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc atctccgcca
    11221 ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac cagatatatg
    11281 gcaatgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac cacagcttaa
    11341 atccgcccgg cggtttcgac tgggcagcag gttcaagtgt attcatcttt gctccttaaa
    11401 ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg ccccaggtcg
    11461 gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctaatcatc tgcccacttt
    11521 tcagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc acctccccca
    11581 ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt tccgggcgca
    11641 gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact ttcagtggat
    11701 gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg agtacacctt
    11761 caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga tgctcgtaga
    11821 tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt cataatggcg atacgccccg
    11881 ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct
    11941 ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc agcgcgccca
    12001 tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc agggccacac
    12061 gctgtcgctg atcaccggaa agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca
    12121 ccagcccaag catctcgttg acccgggtgg caatttccgc tttcggtagt ttgtcctgtt
    12181 tcaggccaaa ggcgatgttc tgctccacag tcatatgggg aaacagcgcg taagacagaa
    12241 acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg ccatcaagca
    12301 ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc gtggacttgc
    12361 cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc aggctgacat
    12421 catccaccgc atgttgacca tcgtaggatt tggtgaggtt gcggatttct aatagcggcg
    12481 tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg gctgctctcc
    12541 ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggcgggt gtggtgcgcc
    12601 cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca cgcgcgggtg
    12661 cgcacacggt cgattttcgg gtcctgcact ttcagagtga acagcttcgc acgaacatcc
    12721 gcaggcggat aaatacccgg gttctcacgg acttccgcac tcaccagcgg cgtggctgct
    12781 ttattggcgt tggcatagaa cacattgtcg gaaatatgcg ctactacatc cgggcgcagc
    12841 aggtaattca ggaactgata ggcttcgtct ttgtttttgg catccgcagg catggcgaat
    12901 acatcaaaga acgccatcgc cccttctttt ggaatcgaga acgagacatt cactccattc
    12961 ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac ctgcccagcc gatagcgacg
    13021 caaatatcgc cgtttgccag gtcgttaatg tattgagatg aatggaaata acgaatgttc
    13081 gggcgcagct ttaacagcag atcggttgcc ggtccggtgt aatcatccgc tttagtgctg
    13141 ttgggatctt tgccgagata attcaacacg gtagcgaaaa cttcttctgg cgcatccagg
    13201 aaagagacac cgcagctttt cagtttttcc agattttcag gcttgaggat caagtcccag
    13261 ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt atagccaatc
    13321 ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg tttggcgacc
    13381 aacttcagca gctccggatc gagattcttc cactccggca atttgctttt gtccagcggc
    13441 tggaaaactc ccgctgtcag ctggcgttca agaaagctgg cagatggaac caccaagtca
    13501 aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc gaaaacatcg
    13561 tagacgactt taataccggt ttctttttca aaattggcca ccgtgtcagg ggcgatataa
    13621 tcagaccagt tataaatgtg gagtgttttt tgttcagccg cgagcgtgcc gacagagacg
    13681 gccatcagag cacccgcaac cagacccgat agccattttt tatttaaggc ggtcatatct
    13741 ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactgaa agatatgcaa
    13801 taaacgtgca tattttttta tcggcgcggt gtaagaaaag agaactctct cccggccagc
    13861 actgctcgct atataaaagc atcgcaagaa taactttagc cagggtttga gactatagct
    13921 cggattaaaa aacggtattt aacaaatttt tatgcgtatt tgctctatgt gataaagcca
    13981 aatcgacaca agataagtga aataatcttt aagaaaaggt taaaaacagc agataatttt
    14041 gcagcgcgca gtcactgcgg cagcgactgc gtgctcgaaa ggaattatca gtggagaaaa
    14101 tagttttcgg taacgttgtt ttgcccttca tcgtcagtcg gtaacagcag ttgatgggca
    14161 ttagcttcaa gaatgaccat cgaaatctgc tcttcgctct gacgcacgaa ccatgcaaac
    14221 tgctcatagg ttacgccctg cataacggat aaagactggc aaaccaccag ctttggcaga
    14281 ttatcatcct gcatatcaag aaatgctttc acggtcagcg aactggcatt gatggcagat
    14341 aaatccgccg ccagcggcaa tacggctgaa ggtcggactt ccgccatggc agaaaacagg
    14401 atcgtgttat cgatcagatc gattttggca tcaaagacac cgtcgaaatt ctgcatatgg
    14461 ggcagatgca gagcctgaca gttatcacat tcaaaaaaac tcatccccag gtcatcgagc
    14521 cattgacgca gcgtatccag accaggaacg accagcgatg tcataatttt gtacaacctc
    14581 tttcgataaa aagaccggca cagcttacgc aaaaagcgca ggcaaaacca tgatcagtaa
    14641 tgtgattgcg actaaccacc cgttttcagg caatattctg tcgtagcgtg gcgttcgatc
    14701 cagcggatca ttttacccgc gatgtcgata ccggtggttt tttctattcc ttccagcccc
    14761 ggcgacgcat tcacctccat caccaacggc ccgcgattag cacgcagaat atccacacca
    14821 gcaacgtcca gcgccatcgt tcgcgcggct tttatcgcga tttcacgctc ctgtggtgtg
    14881 atactggcga cgcttgccgc gccgccacga tgcaaattgg aacgaaaatc gccctctttc
    14941 gcccgccgtt caatcgcagc gaccacttca tcgccaacaa ccagacagcg gatatcgcac
    15001 ccttgcgcct ctttgata
//
LOCUS       DAHBHC010000105        14830 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHBHC010000105 DAHBHC010000000
VERSION     DAHBHC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.106"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(13..804)
                     /locus_tag="M2A35_004309"
     CDS             complement(13..804)
                     /locus_tag="M2A35_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCB7200529.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(810..1937)
                     /locus_tag="M2A35_004310"
     CDS             complement(810..1937)
                     /locus_tag="M2A35_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HCB7200530.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWFTTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQIPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            2466..2738
                     /locus_tag="M2A35_004311"
     CDS             2466..2738
                     /locus_tag="M2A35_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCB7200531.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDHARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDTEASFNWSANTPTHGRR"
     gene            complement(2739..3611)
                     /gene="rluF"
                     /locus_tag="M2A35_004312"
     CDS             complement(2739..3611)
                     /gene="rluF"
                     /locus_tag="M2A35_004312"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCB7200532.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPLVKMEKTSEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            3823..4512
                     /gene="pepE"
                     /locus_tag="M2A35_004313"
     CDS             3823..4512
                     /gene="pepE"
                     /locus_tag="M2A35_004313"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCB7200533.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(4603..6234)
                     /locus_tag="M2A35_004314"
     CDS             complement(4603..6234)
                     /locus_tag="M2A35_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCB7200534.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6454..10137)
                     /gene="metH"
                     /locus_tag="M2A35_004315"
     CDS             complement(6454..10137)
                     /gene="metH"
                     /locus_tag="M2A35_004315"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCB7200535.1"
                     /translation="MSSKVEQLRAQLNQRILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            10337..11161
                     /gene="iclR"
                     /locus_tag="M2A35_004316"
     CDS             10337..11161
                     /gene="iclR"
                     /locus_tag="M2A35_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCB7200536.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDSVTEFGAMVIKAAKEVTLAYGGMR"
     gene            11478..13051
                     /locus_tag="M2A35_004317"
                     /pseudo
     CDS             11478..13051
                     /locus_tag="M2A35_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(13020..14756)
                     /gene="aceK"
                     /locus_tag="M2A35_004318"
     CDS             complement(13020..14756)
                     /gene="aceK"
                     /locus_tag="M2A35_004318"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCB7200537.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
BASE COUNT         3687 a         3964 c         3557 g         3622 t
ORIGIN      
        1 taacgccggg ctttatgccc ggcgcattac cgtctggtac attaagctat ccagttcctt
       61 aacgtcccct tctctgctat ccggtaacag cggtggaatt tccccacgtg cctgttccgc
      121 acgaataaac gcatgtaaca atggacggcg cggaccatat tccgcttctc ctgcgcgctg
      181 tttacgttcc agtaattcgt ctatttctgc acgtaacggc gcatcaagtt cgcttcccgc
      241 cagtaactca gcaaagcgca ttggcggtac gcctttaccc gcttcgaccc agcgcacggc
      301 cagcagcgga cgcagcacgt aaaagtattt tttcagccgc acttcgtcgc cttgtaaata
      361 accgcgaaag tttttccgcg ccatcgaata ataatgccaa cgcgcacgca gcggagaaaa
      421 ccacttcggc accattgcct taagcgccgt tatcgtttct tcatcttgtt gatagaccac
      481 aggagaatcc agccattcta tcagcgtcgg attcgcacct ttcaataatc ccagcgcctt
      541 gcgccactcc cagcctgaaa catctaactc gtcgtcaata ggcaactcaa taacatcgcg
      601 cggcgcctct actcgcagat accattccag cgggtgaaca tacaaaaacc gtacatcgta
      661 gtcgctgtcg ggcgaggcaa atccccagcc gcggctccct gattcacagg cgtacagtac
      721 tttcacatca taccgccgtt caatttctct taactgctgc gatacccttt ctcgcatcgc
      781 agcactgact ccatttaatg gcatgttact caccctttga tattaaccaa cccatgcaga
      841 acctgatcca gaccgccaaa tacagagatg ttatcgatcc gttcagccac tctctccagc
      901 gtttccagtt cttttaagcg taacgctacc gggttgtttt ccatgacttt agcggtattg
      961 agtaacgaac gcgtcgctgc cgtttcttca cgtcggcgaa tcacattggc ctgcgcggat
     1021 ttctccgcct caaccagctg cgcaaggata tttttcatat cgccaggcag gacgatatct
     1081 ttcaccccta acgaggcaat ctccatacca aatggcaaca tgcgcgattt cacctgttcg
     1141 ctgacaacgt catcaatgac ctgtttatct tccagcaatt catccaacgt acgcgtacct
     1201 accgcttcac gcagggcaaa ttgtaattcc cgatacaaat gatcaatcgg cttcgttaac
     1261 tggctaaatg ccaataacac gtcgctgtag cgccagttag cggcgaggtt aatccgcaga
     1321 ttgaccttat ctttcgttaa gatttcttgc ccgctgactt ccagtacctg caagcgggtg
     1381 tcaaccactt cagcgtcaac cagatggtta attttccagt atgcggtcag ccccggcggt
     1441 aacagcgcct gcgtttcacc atcgattttc agcacgccta catgccaggc cggaatctgc
     1501 accgttagta ttgcatcgcg gcctttaact gcccctttgc gccgtggttg caataccgca
     1561 ttcatcacct ccgtttgtac ctggacttgc tgcgtattca tccgcaccag tgtcaaatca
     1621 tcctctacac gccagtacaa acggcgcgtt gatggcggca ggatctcctg caaaataccg
     1681 tccatataca acgccccggc ttcagtctcc gacaagtcgg ccaccacgca atatctttcc
     1741 acccaatcag gctgaaagcg gcgcaaataa tccgccagca cgtcgggcac ttcgctacca
     1801 tccagcgtaa tcagcaacac ttccggggta gtaaaccagg gtaaaatatg ctcgcctgca
     1861 tgtagcactt tgtagtaatc gccgtttctg gagagtaatg ccagctgatc ttttcttacg
     1921 cttattttct taatcattat ctattccttg cttaacagcc gtcgcggaag cggacacgaa
     1981 gcgacagggg aattcccttt tgcctgttac ccggcgtctt tatcggcgac gttatcgggg
     2041 atcgctgact gcgcaatccc tcatttttta tttaataagt tacaaccagt attcatttca
     2101 ggaatgaatg tgggaatcga acccataatt ggcgtcaaag gctaacggaa cacgcccgga
     2161 aaacagcctg accgttcctg gtgtgccgac ggggtttaac cccctttgaa attcagtatc
     2221 aacgcaaaag tgttattgcg agaagcgtgc cagaattgag aaaaattctt aaccaaatga
     2281 aaaataaaga gttagtgaat tacttttgtt tgtaggggga aaacttttcg ctaaatgtgt
     2341 atctgtattt atcgtcaatt atcaagaaag ataaatattt aagaagagaa caaaggaata
     2401 ttatcagttc tgcttgcaaa tgaattcccg tacactgaat ccatcggcta actgagggca
     2461 acatcatggc actcccgcgt attacccaaa aagagatgac agagcgcgag caacgcgagc
     2521 taaaaacgct gctggaccac gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
     2581 ccaacagcat taaaaaagag tacatcgata agctgatggt tgagcgtgaa gcagctgcaa
     2641 aaaaagcccg ccagttgaag aaaaagcagg cttataaacc agatactgaa gcatcgttta
     2701 actggtcggc taatacacca acgcacggca ggcgctgatt aacgcccctt ctttttacgc
     2761 cccggcgatg tgaaacgttt accatttgac gccgggcggc cacctttttc tgatgttttt
     2821 tccatcttca ccaacgggcg tttgatgccc gccgttttcg gtttggcctt cactttaggt
     2881 tttgcctcgg aagaggaatt ttcgatgagc ttaaacaggt cgattaactc atcgtcagtt
     2941 aaatcgcgcc actcacccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt
     3001 tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcagc
     3061 ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt gcactttttc
     3121 gtcaccgtcc cgaggatcgg cacccccgcc cccataccac gaataaattc atcggtaatc
     3181 ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta
     3241 ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg
     3301 ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca
     3361 tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac caaatcttcg
     3421 gcttcccgag gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg tttcacctga
     3481 tcgccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc
     3541 gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag
     3601 tcgggcagca taaattctcc tgtaaaaaac ggaatatacc gtacctttgg gttgataaaa
     3661 aatagtcatg agcgaactat ttgttatttc cattggcttg aattcgttac ctgctcaaat
     3721 cactttccag aagcaatgtg cgacatcgtg agccatgtca gtaagtggtt aaattcgcag
     3781 tggtagcatg cctcccgtga tttcttacta accggagaag taatggaact gcttttattg
     3841 agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct aattgctgaa
     3901 cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac gcagacctgg
     3961 gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt
     4021 attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt gattgtcggc
     4081 ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt
     4141 actgacgtgg ttaaacgtgg cgctctgtat attggctgga gcgcaggcgc taaccttgct
     4201 tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg
     4261 ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat
     4321 aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg
     4381 attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc tacgctgggt
     4441 ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggtgccgct agaagctggt
     4501 caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg
     4561 cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc
     4621 ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag
     4681 gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt
     4741 ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaagcg
     4801 atgcttacta cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat
     4861 tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct tcaagccatc
     4921 aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc ccatgcgctc
     4981 gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg cccagcggcg
     5041 cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct taatggcggt
     5101 gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc
     5161 gtgcatcact ttatttagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt
     5221 ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg
     5281 tttgggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg
     5341 tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg
     5401 gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct ctgccagcaa
     5461 atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac tacccagcgc
     5521 gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca gaccggaacc
     5581 gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt
     5641 cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc
     5701 gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc
     5761 gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gcaaaatcag
     5821 cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa
     5881 aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat
     5941 acgcgccatt agcgccgtcc cgacatctgc acccagcaca atgaccagag ccggtgcgag
     6001 ggctaccaga tcctgagcga caaacgaggt caccagcatg gtggtggcat tactgctctg
     6061 taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg
     6121 gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg
     6181 agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc
     6241 aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac
     6301 gctgactggc atctttactg tgccttttga ccttcgtttt tccggctaag attattccag
     6361 cctgattcca gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct
     6421 gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg
     6481 tgccagccag cgctcaactt cggtaacgct cataccttta cggcgggcat aatcttcaac
     6541 ctgatcgcgc tgaatttgtg ctacagcgta gtacttgctg tccgggtggc tgaagtacca
     6601 acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca tgccagtgtg
     6661 tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg
     6721 atagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag
     6781 gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc
     6841 aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg
     6901 ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc
     6961 gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt
     7021 agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt
     7081 gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc
     7141 caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
     7201 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
     7261 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagagt
     7321 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
     7381 ctgccagtca aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
     7441 cggtttctta cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac
     7501 aaaatcatca cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgatgc
     7561 gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
     7621 ttttgaggtc gtcgcgccgc caatcaatag cgggatggtg aagccctgac gctccatctc
     7681 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag
     7741 atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag
     7801 atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt taccgatgtc
     7861 gtggacatcg cctttcacgg tggcgatcac catcttgccg ttggttttac cctgctcttt
     7921 gctggcttca ataaacggtt cgagatacgc cactgcctgt ttcatgacgc gcgccgattt
     7981 gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca cgttcatacc
     8041 gtccatcagc gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
     8101 ggtatcctgc tcgataaact cggtaatgcc tttaaccagc gagtattcca gacgtttatt
     8161 cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc
     8221 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcgcgac ggttaagaat
     8281 cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg ccagttgccc
     8341 ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg
     8401 aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacaccgcc
     8461 ggaaatcagc gcgtgcggca gttcgcgttt gatatcttca cacgcgccga taaagtcctg
     8521 cgcgtagttg ttgtgctctt caatgccggt tgcgaccgcg aagatgttcg ggtcgaagat
     8581 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
     8641 ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac
     8701 tgccgcaccg taacgacgca agagcttcgc gtgatggata aaggcgtcga cgccctcttt
     8761 catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt tttcaatgac
     8821 atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
     8881 cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatgttgat
     8941 atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagtg cctcgctgta
     9001 tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc
     9061 cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca
     9121 ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
     9181 tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa aacccgcttg
     9241 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc
     9301 gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc
     9361 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagagc
     9421 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
     9481 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
     9541 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
     9601 caggatcaga tccgcgccac cttccaccag cgctttggtg gactctcgat aagccgccac
     9661 cagcccgtca aaagtgatat tacgaaatgc cggatcattt acgtccggag aaatagacgc
     9721 cgtgcggttg gtcgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
     9781 ggtccactca tcagcacaag ctcgcgccag tttcgccgcc gcaaagttga tttccgccga
     9841 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
     9901 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
     9961 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
    10021 accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc
    10081 cagcaccaga atacgttgat ttaactgcgc acgcagttgt tccactttgc tgctcacact
    10141 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
    10201 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
    10261 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt ccacgataca
    10321 gaaaaaggag gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
    10381 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
    10441 gtggattgcc gaatccaatg gcagtgtggc actcacagag ctggcgcaac aagccgggtt
    10501 acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca
    10561 ggtcggcgaa ctgggacatt gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
    10621 tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatt taatggaaga
    10681 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
    10741 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
    10801 gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
    10861 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
    10921 acatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
    10981 acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gcgaaccgtt
    11041 tgccgcaatt tcaatttccg gaccgatttc acgcattacc gatgacagcg tgaccgagtt
    11101 tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg
    11161 actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa
    11221 gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt
    11281 tattaactca gcattatttt taaacatcaa accacttaat cattaacaaa atcgcaagat
    11341 ctcattccac cgaaactttt acggctgcat tatgagattg agcgatttat gatatttctt
    11401 tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa gacatcaata
    11461 aacaatgaga tatttaaatg attactcgta tccctcgtag ttctttctct gcaaatatta
    11521 ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg
    11581 aggataaatt ttctggcaaa gagctggcga cgcctctatt aaaaagcttc tcagagaact
    11641 gtagacaaaa tggtcgtcat atctttagta acaaggattt tgtcattaaa ttttccacgt
    11701 ccgtcttaca agctgataag aaagaaatta cgataattaa taaaaacgaa aacacgacac
    11761 ttactcaaac cattgcccca atatttgaag aatacctaat ggaaatttta cctcaacgct
    11821 cagacactct tgataaacaa gaattaaacc taaaatcaga tagaaaagaa aaagaattcc
    11881 caagaattaa acttaatggt caatgttatt ttccggggcg accccaaaac cgtatagtat
    11941 gccgacacat tgctgcacaa tatattaatg atatttatca gaatgttgat tacaaacccc
    12001 atcaagatga ttactcttca gctgaaaaat ttctcacgca cttcaacaaa aaatgcaaaa
    12061 accagacttt ggcgttggtt tccagccgtc ctgaggggcg ttgcgttgct gcctgcggtg
    12121 atttcgggct agttatgaaa gcatattttg acaagatgga atcaaatggc atcagtgtta
    12181 tggcagccat attactggtg gataaccatg ctttgacggt ccggctaaga ataaagaaca
    12241 caactgaagg atgtacccat tacgtggttt cggtttatga tcctaatgta actaacgata
    12301 aaataagaat tatgagcgaa agcaaagagg atattaaaca ctattctctg atggatttta
    12361 tgaatgtaga ttatagcctc ctgaaatggt caaatgatca tgttattaac caatctgttg
    12421 caataattcc agcacttccg aaagaacagc tattgatgtt aaaaggatct gtggatgaaa
    12481 taacccctcc attatcacca gcaacgatga atttgctaat ggcaattggt cagaatcacc
    12541 aacttacgca actgatgatt caactccaga aaatgccaga actacataga acagaaatgt
    12601 tgactgccta taatagtgga catatgaacg ttattaatac tatttttaac gcattaccca
    12661 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    12721 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    12781 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    12841 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    12901 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    12961 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    13021 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    13081 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    13141 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    13201 cgaaactctt ccgggaaaac atcgcccgga gagacgctgt accacggttc gctggcaagt
    13261 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    13321 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    13381 agcatgtcgc caggaaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    13441 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    13501 atccgccgct cgatatagag atggcgaatt acaatctgct caccaagatc ggtgattttt
    13561 tccgctgctt cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc
    13621 acaaagtttt caaactcctg ggtgtccgcc attcggccca cgcgatcgtg ctctttcacc
    13681 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
    13741 ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa acaccaacat caccattcca
    13801 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
    13861 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg
    13921 gttttacctg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa
    13981 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg
    14041 caggtatcaa taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac
    14101 gtgccggaag gcgtgatcag tttgcccacc agccaggcgg ctttattgcg gtaaaacagt
    14161 tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga
    14221 tggcgaatga tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg
    14281 tcgctgataa cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc
    14341 agcgggcggg gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc
    14401 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
    14461 gcaatctcga agcgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt
    14521 aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga
    14581 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
    14641 tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
    14701 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
    14761 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
    14821 tcggcaacgg
//
LOCUS       DAHBHC010000106        14736 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHBHC010000106 DAHBHC010000000
VERSION     DAHBHC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.107"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..278
                     /locus_tag="M2A35_004319"
     CDS             <1..278
                     /locus_tag="M2A35_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCB7200538.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="M2A35_004320"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="M2A35_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCB7200539.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQYNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDTVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDVQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDVMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     KATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEYSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPAREQAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="M2A35_004321"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="M2A35_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCB7200540.1"
                     /translation="MTTDNTNTTRNDSLAARTDTRLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAMIAQF
                     AYYLADFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWVPFSGTSYCIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6369..6929
                     /locus_tag="M2A35_004322"
     CDS             6369..6929
                     /locus_tag="M2A35_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCB7200541.1"
                     /translation="MTEQKRPVLTLKRKTEGTGPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7062..7262
                     /locus_tag="M2A35_004323"
     CDS             7062..7262
                     /locus_tag="M2A35_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200542.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAAR
                     VAPRTDWQQFARQRAERCQRRR"
     gene            7648..8247
                     /locus_tag="M2A35_004324"
     CDS             7648..8247
                     /locus_tag="M2A35_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200543.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNN
                     VALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYD
                     GIVRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTF
                     NNYLKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
     gene            8411..8641
                     /locus_tag="M2A35_004325"
     CDS             8411..8641
                     /locus_tag="M2A35_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200544.1"
                     /translation="MTVAVRGKQEFIIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTL
                     PARFCREILPDDVRSITIILEKSDDEWWYGSY"
     gene            8906..9160
                     /gene="repA"
                     /locus_tag="M2A35_004326"
     CDS             8906..9160
                     /gene="repA"
                     /locus_tag="M2A35_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCB7200545.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            9399..9473
                     /gene="tap"
                     /locus_tag="M2A35_004327"
     CDS             9399..9473
                     /gene="tap"
                     /locus_tag="M2A35_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCB7200546.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            9466..10323
                     /gene="repA"
                     /locus_tag="M2A35_004328"
     CDS             9466..10323
                     /gene="repA"
                     /locus_tag="M2A35_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCB7200547.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            11241..11525
                     /locus_tag="M2A35_004329"
     CDS             11241..11525
                     /locus_tag="M2A35_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCB7200548.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMA
                     CKAAEDIILDRRVFNFNDRQYEEFIEMLDAPVADETAIEKLLARKPQWEV"
     gene            11513..12001
                     /locus_tag="M2A35_004330"
     CDS             11513..12001
                     /locus_tag="M2A35_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7200549.1"
                     /translation="MGGVTAPEPLSSFHQAAEFVIGEAVLDDWLKQKGLKNQAPGATR
                     TFVVCRKGSQQIVGFYSLATGSVNHTEAAGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKSQVQTLFLKLP
                     GK"
     gene            complement(12299..13015)
                     /locus_tag="M2A35_004331"
     CDS             complement(12299..13015)
                     /locus_tag="M2A35_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200550.1"
                     /translation="MPGVCYFILIKKPITFKFLNDFMALNSGDVLLVKHTEREFLQSH
                     SEKYSEFELTDDNVRNYLSCCKHREKQFAGKTPCILRKTFAYPDLLINIIDNINDKDH
                     IHPDFKDAITYSLLSIFSDEEKLKSFLTSGMPTFSGKVRSILLSDVSRHWKLRDLSDY
                     LYMSESLIKKKLLSENTSFSKLLLDTRMSFALKMLKQNHPVKYVSEMCGFSSTSYFVC
                     LFRQYYECTPGKYIKNQKSS"
     gene            13252..13902
                     /locus_tag="M2A35_004332"
     CDS             13252..13902
                     /locus_tag="M2A35_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCB7200551.1"
                     /translation="MEVVYIAEPVLSPDTNKLIAVEVLSRFYSESGCSLSTQMVLSMF
                     TSTMKINLLNSQLNAIVKFRDFFERHGILCSVNVDYETCLYIRRDIQTQETILKNKFL
                     VLEISETYPVLTQEYDVIPFLLSLTEYIWLDDFGSGNSTMSSAMKNKYHAIKLDKLFY
                     QNNTGKPHFKVFVEELKKVCPNIIAEGIENIHYNKLSKEAGLWGGAGIFFSFCAAV"
     gene            14253..14508
                     /locus_tag="M2A35_004333"
                     /pseudo
     CDS             14253..14508
                     /locus_tag="M2A35_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            14505..>14736
                     /locus_tag="M2A35_004334"
     CDS             14505..>14736
                     /locus_tag="M2A35_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7200552.1"
                     /translation="MTYTVKFRDDALKEWLRLDKAIQRQFAKKCSENPHIASAKLRGL
                     KDCYKIKLRASGFHLVYQVIDDMLIIAVVAAGK"
BASE COUNT         3768 a         3444 c         4184 g         3340 t
ORIGIN      
        1 tgtcttctgt catcaatgat aagaagtcag atgcaggtgt gagtgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc
      181 atccggacat ccagcagcag atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg atgagtatcg cgcaggtcag
      301 atcggccgga agtgccggta actattatac cgacaaagat aattactatg tactgggcag
      361 catgggagaa cgctgggccg gcaagggggc tgaacagctg ggactgcagg gcagtgtcga
      421 taaggatgtt tttacccgtc ttctggaggg aaggctgccg gacggagcgg atctaagccg
      481 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgttaggtgg cgataagcgc ctgattgatg cacataacca
      601 ggccgtggac tttgctgttc gtcaggtgga ggcgctggcc tccacacggg tgatgacgga
      661 cggacagtca gaaacggtac tgaccggtaa tctggtgatg gcactgttta accacgacac
      721 cagtcgcgat caggaaccac agttacacac gcatgcggtg gtggctaatg tcacgcagta
      781 taacggtgag tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa
      841 tgtgtacgct aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt
      901 tgaggcgctg ggctatgaaa ccgaagtggt gggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccggacgctc acaggctatc cgggaggccg tcggggaaga
     1021 tgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcatat cgtgacgcgg cggatcagcg tgcggagacc cgcacgcagg cgcccggggc
     1201 tgtttcacag gaggggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtatacgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaaaat ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc ccccttgacc gtgagaaggg gctgttcaca tccgggattc atgtgctcga
     1441 tgagctgtca gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca
     1501 tccggagaaa agtgtccccc ggacggccgg ttacagcgat accgtcagtg ttctggcaca
     1561 ggatcgcccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaagtgcaga ttatcgctgc
     1681 tgaccgtcgt tcgcagatga acctgaagca ggatgaacgg ctgtccggtg agctgataac
     1741 cggacgtcgt cagctgctgg aaggcatggc cttcacgccg ggcagtaccg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggacggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat
     1921 ggccatgaag gatgccgggg tgaacacata tcgctggcag gggggagagc agcgaccggc
     1981 aaccatcatc agtgaaccgg accgtaatgt ccgctatgcc cggctggcag gggattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     2101 catactgacg caggccattc gcagtgagct gaaaacacag ggcgtgctcg gacatccgga
     2161 ggtgaccatg acggcactgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccgt ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgtgata gaccgggtga cagcgcagag tcacagcctg accctgcggg atgtgcaggg
     2341 cgaaacgcag gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgacc gggaaaattc ccggactccg
     2461 agtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgtga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggcctccct gcctgtgagc gattcaccgt tcacggcact
     2581 gaagctggag aatggctggg tggaaacgcc cgggcattcc gtcagcgaca gtgcgaaggt
     2641 ttttgcctcc gtcacacaga tggcaatgga caaggccacc ctgaacggtc tggcccgcag
     2701 tggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     2761 ccgccatccg tcctttacgg tggtttctga gcagataaag gcgcgggccg gtgagacatc
     2821 gctggaaacg gctatcagtc atcagaagtc cgcacttaac acaccggcac agcaggccat
     2881 tcatcttgcc cttccggtgg tggaaagtaa aaacctggcc ttcagccatg tggacctgct
     2941 gacagaggca aagtcgtttg ctgcagaagg aaccagtttt gctgacctgg ggcgggaaat
     3001 caatgcgcag ataaaacggg gcgatttact gcatgtggat gtggcaaaag gttatggcac
     3061 agacctgctg gtttcccgtg cgtcgtatga ggctgaaaaa agcattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagagta cctggcgaac tcatggagaa
     3181 actgacatca ggacagcgcg ccgccacccg catgatactg gaaacgcccg accgtttcac
     3241 ggtggtgcag ggctatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaacatg ctgccggaga gtgagcgtcc ccgtgtcgtt gggctgggcc ccacgcaccg
     3361 tgcggtcggg gagatgcgca gcgccggcgt ggatgcacag acactggcgt cctttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacaccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggc ggtcgggctg tagccagcgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagccc ttccgtcttc agcagacgcg cagtgctgcc gatgtggcta tcatgaaaga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggtgccac gtctggaggg
     3781 cgcatgggca ccggagtact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg cgaggctttc ccggatgtcc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg cagaacaccg gaagcacggg agcagacgct
     3961 gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga acacagcgaa
     4081 tatacgcgac ggggagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 cgtcacactg tacaccccgg acaccatccg ggtgggaacc ggtgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccgcccggga
     4441 gcaggcagag caacatattg acctggccta tgccatcacc gctcacggtg cgcagggggc
     4501 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt ggccggaaac agatggccgg
     4561 ctttgagtca gcctacgttg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccattaa caatgccgta cagaaaggaa ctgcccacga
     4681 tgtgctggag ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 gcgggagctg cgggacgtgg cggcagggcg tgccgttctt cgtcaggcgg ggcttgccgg
     4801 gggagacagt cctgcacggt ttattgctcc gggacgtaaa tatccacagc cgtatgtggc
     4861 actgccggcg tttgaccgta acggcaagtc tgccggtatc tggctgaacc cactgaccac
     4921 ggatgacgga aacgggctgc ggggattcag tggtgaaggc cgtgtgaaag gcagcgggga
     4981 tgcgcagttc gtggccctgc agggcagccg taacggcgag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa tcccgacagc ggtgtggtgg tgagaatcgc
     5101 cggtgaaggc cgtccgtgga atcccggtgc cataaccggt ggccgcgtgt ggggggatat
     5161 cccggacaac agcgtccagc cgggagccgg aaatggcgaa ccggtcacgg cagaggtact
     5221 ggcacagcgg caggctgaag aggccatccg ccgtgaaacg gaacgccgcg cagatgaaat
     5281 tgtccgtaaa atggcagaga acaaacccga cctgccggat ggcaaaacag agcaggctgt
     5341 cagggaaatt gccgggcagg agcgtgaccg ggctgccata actgaacgag aagccgcgct
     5401 gccggagagt gtgctgcgtg aatcacaacg ggagcgggaa gcggtccggg aggttgcccg
     5461 ggaaaatctg ttgcaggagc gactgcagca gatggagcgg gatatggttc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagaca aacgctgatg acgacggata
     5581 acacaaacac aacacgtaac gattcactgg ctgcccggac tgatacccgg ttgcagtcgt
     5641 tgctggtctg gtcacccgga cagcgggata ttatcaaaac ggtggcactg gtgctgatgg
     5701 tactggacca tgcaaaccgc attctgcatc tggaccagac gtggatgttt ctggccgggc
     5761 gtggagcctt tccgcttttt gccctggtat gggggctgaa tctgtcccgc catgcgcata
     5821 tccggcaacc ggccattaac cggctgtggg gatgggcgat gattgcccag ttcgcatatt
     5881 atctggccga ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggcggcac
     5941 aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc attctcctga
     6001 tggcactgtg ggtgcctttc tccggcacca gttactgcat tgccgggctg ctgatgctgg
     6061 cagtcagcca ccggctgtac cgggcggaag acagagcgga acgtctggcg ctggtcgcct
     6121 gcctgctggc tgttattccg gcgcttaatc ttgccaccag tgatgccgcg gcggtggccg
     6181 gtcttgtgat gacggtgctg accgttggtc tggtatcctg tgcaggaaaa tcattacccc
     6241 gtttctggcc cggagatttt ttcccgacgt tctatgcctg tcatctggct gtgctgggcg
     6301 ttctggcact gtgacgggtg tggtatcttt ggctgcaaga ggatgattcg tcagaggcag
     6361 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaag acagagggaa
     6421 cagggcctgt ccgcagccgg aaaaccatca tcaatgtcac cacaccacca aaatggaagg
     6481 tgaaaaagca gaagctggcg gagaaagcgg cccgggaagc agagctggcg gcaaaaaaag
     6541 cgcaggccag acaggcgctg tccatttatc tgaacctgcc ctcactggat gaggcagtga
     6601 ataccctgaa gccctggtgg ccgggactat ttgacggtga cacgccccgg cttctggcct
     6661 gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata
     6721 aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga
     6781 aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg
     6841 aggaagcgta tgcggcagcg cgtctggata aaatccgccg ccagaaccgg ataaaggcag
     6901 aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc atcagagaga ggaggtaggg
     6961 aaataaggct taaaaggaaa taacttccgt acccgaaagg aagccaattc agtagattat
     7021 ttaacaccat aatactgacg ggttaaagag gagtgataaa gatgaatgga ttcagaaaca
     7081 gttcacggaa cggtcaggtc tggcgctggc agcgtgccgg aagccgtgcc gtcagcctgg
     7141 aggtcagggg attccggaag gaggccgctg aagcgtggct cctggctgcc cgtgttgctc
     7201 cccgtacaga ctggcagcag tttgcacggc agagagccga acgctgtcag cgacggagat
     7261 aatgaaaaaa aacggcaaaa aaagacttcc cctgagcaaa ggaggccaaa aatcattcat
     7321 agcgtatcac tgacattgaa tgaatgctct gtacatcaac cctgtgaagc taccaccggt
     7381 taatgccggg aggttgcggg aagaaagata atacacagtc attacgtggt taatatctgt
     7441 cagaatgttt tttgtgacca taaactgtca gatggataaa aaaagaccgt catgtgacgg
     7501 tcagtgttgt cctgtcaggg acaaatgcat tcagtaagga gagcaaaata ataacatcac
     7561 gttgcccggc tgttaatatc aggcgatgtt aagtgttatt tatgactctt ttatctggtt
     7621 gtgaagtctg ttaatcagga agagaaaatg gcgaccggaa aatcagagaa gcaacaggtg
     7681 attgccttta tcgattacga gaactgcagt aacctgacag aaatttcctt aaatatatat
     7741 acggagttaa ttatattcgc gggtgcgaaa cagaataatg tggcactgcc agtaaacgct
     7801 ttccctgaag cagtaaaaat cacactccgg catgtcagtg aagtctcccg gaacaatgtt
     7861 gattttcatc tggtgctgga gctggggcag tgtgtatgcc actacgggcc ggagaaggaa
     7921 taccatattg tttccgctga caagggatat gacggtattg tccggacact gcaggcgcgc
     7981 gggattcgct gccgtcgcgt ctccccggat aaggcgtcat cagtaaagat ggctgcaccc
     8041 gacatggata tcgttatccg gtgggcaaac aaaattcaac aggcagcgcg ggaggttcgt
     8101 aatatgccgg taacagaaaa gacatttaat aattatttaa aatcacagat gcgcggtgaa
     8161 tggcgtgatg tcctgaccag aggtatccgg gatgaactgg tccgtcgcgg ggtaatgaaa
     8221 attcagggga ataaaatcat ctggtaagac tgccagaata aaatgatatt tattttattt
     8281 ataaaaacag aaaggtcgaa aatcaataat ggcatttatt tccatgacaa gacagaaagc
     8341 gccgtacaga tatgcaagca agacccggtt atcgataaga cgaacagact caggctctgc
     8401 cgggtgttat gtgacggttg ccgttcgggg gaagcaggaa tttattattg ctgaacttga
     8461 ttcagaaatc cggaaaatca ggctgaaact gacggactcc tatgaagaag gggtaaggct
     8521 gtcttcgggc acgtttacgc ttccggccag attctgccgg gaaatactgc cggacgacgt
     8581 caggagtatc accattattc tggaaaaaag cgacgatgag tggtggtacg ggagttactg
     8641 atatgtggca gaactccgga cactgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     8701 acgggaaaaa gcaagttttc cccgctcccg gcgtttctat aactgaaaac catactattt
     8761 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     8821 ttcgtcacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     8881 cctgtttaag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc
     8941 tggcgcaaaa cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt
     9001 atcagtggcc cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta
     9061 taaggccatg ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac
     9121 cgcactgata aaaagtgaag cgcaaaaacg atgtatgtga tgatgggctt acattcttga
     9181 gtgttcagaa gattagtgct agattactga gcgtttaagg aattttgtgg ctggccacgc
     9241 cgtaaggtgg cagggaactg gttcttatgt ggatttacag gagccagaaa agtgaaaacc
     9301 ccgataatct tcgtcaagtt tggcgactaa gagaagatta acggggccat ctaaaaaccg
     9361 tatagccaac aattcagcta tgcggggagt atagttatat gcccggaaaa gttcaagact
     9421 tctttctgtg ctcactcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     9481 caaacgtatt accgccaggt aaagaacccg aatccggtgt ttacaccccg taaaggtgcc
     9541 ggaacgctga agttctgcga aaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     9601 gatttcgcca ttcatgtggc gcatgcccgt tcccgtggtc tgcgtcggcg catgccaccg
     9661 gtgctgcgtc gacgggctat tgatgcgctg ctgcaggggc tgtgtttcca ctatgacccg
     9721 ctggccaacc gcgtccagtg ttccatcacc acactggcca ttgagtgtgg actggcgaca
     9781 gagtccggtg caggaaaact ctccatcacc cgtgccaccc gggccctgac gttcctgtca
     9841 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     9901 accgacatca cgttcacacc ggctctgttt gctgcccttg atgtgtctga ggatgcagtg
     9961 gcagctgcgc gccgcagtcg tgttgaatgg gaaaacagac agcgcaaaaa gcaggggctg
    10021 gataccctgg gtatggatga gctgatagcg aaagcctggc gttttgtgcg tgagcgtttc
    10081 cgcagttacc agacagagct taagtcccgt ggaataaaac gtgcccgtgc gcgtcgtgat
    10141 gcggacaggg aacgtcagga tattgtcacc ctggtgaaac ggcagctgac gcgcgaaatc
    10201 gcggaagggc gcttcactgc caatcgtgag gcggtaaaac gcgaagtgga gcgtcgtgtg
    10261 aaagagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc
    10321 tgaacctgac ctcctctgaa taatccggcc cgcgccggag gcttccgcac gtctgaagcc
    10381 cgacagcgca caaaaaatca gcaccacata caaaaaacaa cctcatcatc cagcttctgg
    10441 tgcatccgcc ccccccctgt tttcgataca aaacacgcct cacagacggg gaattttgct
    10501 tatccacatt aaactgcaag ggacttcccc ataaggttac aaccgttcat gtcataaagc
    10561 gccagccgcc agtcttacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt
    10621 aaactactta attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag
    10681 acagacatac acctcccacc gcaagcggcg ggccccgacc ggagtcaatt tagttacaac
    10741 atgcaaatat agcccccgga aaaaaaccag ttccacgcag aactgaaacc acaaagcccc
    10801 tcctcataac tgaaaagcgg ccccgccccg gcccttcggg ccggaacaga gtcgctttta
    10861 attatgaatg ttgtaactaa gcatctttat cgctgtcagt gttctggctg gaagtgttaa
    10921 gtacacgctc gtaagcggcc ctgacggccc gctaacgcgg agatacgccc cgactgcggg
    10981 taaacccttg tcgggaccac tccgaccgcg cacagagact ctgtcatggc tgaaagcgga
    11041 tttggcttta cagggccggg ggtgggtaag gtgaaatcta tcagtacgta ccggcttacg
    11101 ccgggctccg gcggttttac tccggtatca tatgaaacaa ctgagtgccg ccttccatgc
    11161 cgctggcgcg gcatcagtaa cctgtgactt tgtgtatatc atttgctata tgtcgtcact
    11221 gtgctattgt tgtaaagcaa atgatatacg gaggctttat gaaatcaggt gttcagctca
    11281 atctcagggc aagagaatct cagcggatac tcattgatac cgctgcagaa attcttcata
    11341 agtcgcgtac agacttcatt ctggaaatgg cctgtaaagc tgctgaagat attattcttg
    11401 accggcgggt gtttaatttt aatgacaggc aatatgaaga gttcatcgaa atgcttgatg
    11461 ctccggttgc tgatgagact gccatcgaaa aactgctggc gaggaaacct caatgggagg
    11521 tgtaacagca ccagaacctt tgtcctcatt tcatcaggcc gctgaatttg tcattggtga
    11581 agctgtgctt gatgactggt taaagcagaa ggggctgaaa aatcaggctc ccggagccac
    11641 acggactttt gttgtatgca gaaaaggttc gcaacaaata gtcggttttt actctctggc
    11701 caccgggagt gttaatcata cagaggctgc aggcaacctt cggcgcaata tgcctgaccc
    11761 tatacctgtt atcatacttg cacgcctggc cgttgatgtg tcattccgtg gaaaagggct
    11821 tggcgcagat ttacttcatg atgctgttcg tcgttgttac agggttgccg aaaatatcgg
    11881 tgtgcgtgca attatggttc atgcgcttac ggaaaatgca aaacagtttt atattcatca
    11941 tggttttaag ccatcaaaaa gccaggtgca aacactgttt ctgaaactcc ccggcaagta
    12001 acaataatta aaactgtact ttttcttttc ctgtatccgg tttcctgtca gtcacttctt
    12061 ttttgtgcgc atgaaaagaa gcctgcccgg agggcaggca taacagacaa ttgcacagtc
    12121 agtgttgaaa ttaatgatgt cacacatttt cccggtggat attcaaagca tcctgtccat
    12181 tgactgttcg ggaaagccct tatttatagt acaatatttg aaatccaggc tgctccctgt
    12241 catgtcgcag gtgagctctg agtgagttta tcgggccatt gaaatatata tattatcatc
    12301 aggatgattt ctgattctta atatatttac ctggtgtgca ttcataatat tgcctgaaga
    12361 ggcatacaaa atatgatgta ctactaaacc cacacatttc agatacatat ttgaccgggt
    12421 gattttgttt tagcatttta agagcaaacg acattctggt atcaagtaat agtttactaa
    12481 aagaagtatt ttctgacagt agttttttct ttattaaact ctcgctcatg taaagataat
    12541 cagataaatc tctcagtttc cagtgtctgg atacatctga aagaagaata cttcttactt
    12601 ttcctgaaaa cgtgggcatg ccggaggtga ggaatgattt taatttttct tcatcgctaa
    12661 atattgaaag caatgaataa gttatcgcat ctttaaaatc cggatgtatg tgatctttgt
    12721 catttatatt atctattata tttattaata aatccggata agcaaaagtt ttccttaata
    12781 tgcatggtgt ttttcccgcg aattgttttt cacggtgttt gcaacacgat aaataattcc
    12841 tgacattatc atcggttagt tcaaattctg aatatttttc tgaatggctc tgtaaaaatt
    12901 ctctttcagt gtgcttaacc agcagaacat caccagaatt taatgccatg aagtcattaa
    12961 gaaatttaaa cgttattggt ttctttatga gaataaagta acacactcca ggcatggtat
    13021 ccacatctcc ccagtttttt tcagcatatg ttgatactga aacagaaatc aaggttatgc
    13081 gcaaaaaata aatttattga tatatgtcac gttttggggg tgtctgaatg gaacattcct
    13141 ggattatgtg cagataaata acacaggaat gtaaaaacat gggtaagagg gttatggcca
    13201 gtttttagtc ataacgtttc cggaatcagt ctgctttcag gagatgtgta tatggaagtg
    13261 gtttacattg cagagccagt tctctcacca gacacgaata aactcattgc cgttgaagta
    13321 ttgagccggt tttattctga aagcggctgt tctctctcca cgcagatggt gttaagcatg
    13381 tttacttcca caatgaaaat caatcttctg aattctcagc tcaacgcaat agttaagttc
    13441 agggactttt ttgaacgtca tggtatacta tgctctgtta atgttgatta cgagacgtgt
    13501 ctgtatataa ggcgtgatat acagactcag gaaacaatac taaaaaataa gtttttagta
    13561 ctggaaatat ccgaaacata tccggtgtta actcaggaat atgatgtgat accattcctc
    13621 ctctctctta cagagtatat ctggcttgat gattttggta gtggaaacag caccatgagc
    13681 tcagcaatga aaaataaata tcatgcaatc aagcttgata agttatttta tcagaacaat
    13741 acaggtaaac ctcattttaa ggtgtttgta gaggagttaa aaaaagtatg ccccaatatt
    13801 atagcagaag gtatagaaaa tatccattat aacaaattat caaaggaggc tgggttatgg
    13861 gggggggcag ggatattttt ttccttctgt gccgctgtct gaaattaaac aaataaatag
    13921 cgcgtggctg taaccatgtt atattccatc agttatccga atctccgtca tttatcaggc
    13981 tccgtgttct gtcagaacgc aaaaaatatc tatgatacat gctcgttctt ctgtctttca
    14041 gcaaaagaat attcctctta tgtatcattc agaactaatg tggcatatgc gggaaagcag
    14101 acttgtttat tgtcagggct gtgcccggga gcatgaggga attaaatgaa acaggtaacg
    14161 tggttattca cctgcacctc cccatgccag gcttttcagg cggtctttca gagtataatc
    14221 tgaagatctg ttaaatggtc ctgaggtgtg ttatgcccgg tatcattttg tctgacacca
    14281 gcgcaggtgt cagtgagctg aaaaaaaatc cgatggcaac cgtcagtgcc ggagaggagt
    14341 atcctgttgc cattcttaac cggaatcagc ctgtattctg ctgtgtccct gctgagtgag
    14401 ctctatgaaa gaatgcctga tgccctggat gaccaggagc aggtgaaact ggttactgaa
    14461 cgcagcagtc atcctctgca tgatgtggac ctggacggtt atttatgact tatacagtaa
    14521 aattccggga tgatgcgctg aaagaatggc tcagactgga taaggccatt cagcggcagt
    14581 tcgcaaaaaa atgcagtgaa aatcctcata tagcctctgc aaaactacgt ggattaaagg
    14641 attgctacaa aatcaaactt cgcgcatcag gttttcatct ggtttatcag gttattgatg
    14701 acatgttaat tattgcggtc gtcgcagcag gtaaac
//
LOCUS       DAHBHC010000107        14512 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHBHC010000107 DAHBHC010000000
VERSION     DAHBHC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.108"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..189
                     /locus_tag="M2A35_004335"
     CDS             <1..189
                     /locus_tag="M2A35_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCB7200553.1"
                     /translation="ICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQEL
                     ADHNMYQAKHQRAEKLVR"
     gene            205..687
                     /locus_tag="M2A35_004336"
     CDS             205..687
                     /locus_tag="M2A35_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCB7200554.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTS
                     "
     gene            complement(764..1111)
                     /gene="rplS"
                     /locus_tag="M2A35_004337"
     CDS             complement(764..1111)
                     /gene="rplS"
                     /locus_tag="M2A35_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCB7200555.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1153..1920)
                     /gene="trmD"
                     /locus_tag="M2A35_004338"
     CDS             complement(1153..1920)
                     /gene="trmD"
                     /locus_tag="M2A35_004338"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCB7200556.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(1951..2499)
                     /gene="rimM"
                     /locus_tag="M2A35_004339"
     CDS             complement(1951..2499)
                     /gene="rimM"
                     /locus_tag="M2A35_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCB7200557.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTIRSIEVDWDPGF"
     gene            complement(2518..2766)
                     /gene="rpsP"
                     /locus_tag="M2A35_004340"
     CDS             complement(2518..2766)
                     /gene="rpsP"
                     /locus_tag="M2A35_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCB7200558.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(2903..4264)
                     /gene="ffh"
                     /locus_tag="M2A35_004341"
     CDS             complement(2903..4264)
                     /gene="ffh"
                     /locus_tag="M2A35_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCB7200559.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4431..5222
                     /locus_tag="M2A35_004342"
     CDS             4431..5222
                     /locus_tag="M2A35_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCB7200560.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5243..6529
                     /locus_tag="M2A35_004343"
     CDS             5243..6529
                     /locus_tag="M2A35_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCB7200561.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            6652..7257
                     /locus_tag="M2A35_004344"
     CDS             6652..7257
                     /locus_tag="M2A35_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HCB7200562.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(7292..7882)
                     /gene="grpE"
                     /locus_tag="M2A35_004345"
     CDS             complement(7292..7882)
                     /gene="grpE"
                     /locus_tag="M2A35_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCB7200563.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            8005..8883
                     /gene="nadK"
                     /locus_tag="M2A35_004346"
     CDS             8005..8883
                     /gene="nadK"
                     /locus_tag="M2A35_004346"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCB7200564.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8969..10630
                     /gene="recN"
                     /locus_tag="M2A35_004347"
     CDS             8969..10630
                     /gene="recN"
                     /locus_tag="M2A35_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCB7200565.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10779..11120
                     /gene="bamE"
                     /locus_tag="M2A35_004348"
     CDS             10779..11120
                     /gene="bamE"
                     /locus_tag="M2A35_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCB7200566.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(11182..11472)
                     /locus_tag="M2A35_004349"
     CDS             complement(11182..11472)
                     /locus_tag="M2A35_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCB7200567.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(11462..11899)
                     /gene="ratA"
                     /locus_tag="M2A35_004350"
     CDS             complement(11462..11899)
                     /gene="ratA"
                     /locus_tag="M2A35_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCB7200568.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            12070..12552
                     /gene="smpB"
                     /locus_tag="M2A35_004351"
     CDS             12070..12552
                     /gene="smpB"
                     /locus_tag="M2A35_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCB7200569.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12767..13129
                     /gene="ssrA"
                     /locus_tag="M2A35_004352"
     tmRNA           12767..13129
                     /gene="ssrA"
                     /locus_tag="M2A35_004352"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            13402..13650
                     /locus_tag="M2A35_004353"
     CDS             13402..13650
                     /locus_tag="M2A35_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCB7200570.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(13758..14330)
                     /gene="cdtC"
                     /locus_tag="M2A35_004354"
     CDS             complement(13758..14330)
                     /gene="cdtC"
                     /locus_tag="M2A35_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I subunit CdtC"
                     /protein_id="HCB7200571.1"
                     /translation="MKTVIVLFVLLLTGCASEPANQRNLLTQFVGNNAPVDPEPSPVL
                     VNIRNVLTGGIIRNPVGSDFNVNNWVISEVKTNDLDLISAPGGHVQIKNPDGNECFAI
                     LNGQLAVAKQCSESDRNALFTFITSDTGAVQIKSIGSGQCLGNGESITDFRLKKCVDD
                     LGRPFDTVPPGLLWMLNPPLSPAIMSPLTS"
     gene            complement(14327..>14512)
                     /locus_tag="M2A35_004355"
     CDS             complement(14327..>14512)
                     /locus_tag="M2A35_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734593.1"
                     /note="Cdt from Campylobacter forms the A subunit (CdtB)
                     that is delivered into host cells by the heterologous B
                     subnit (CdtA/C); in Salmonella, CdtB alone is encoded;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin subunit B family
                     protein"
                     /protein_id="HCB7200572.1"
                     /translation="IIAPPDPTQASGGVLDYAVVGNSVSFVLPLLRASLLFGLLRGQI
                     ASDHFPVGFIPGRGARR"
BASE COUNT         3753 a         3779 c         3379 g         3601 t
ORIGIN      
        1 atatgctcag cactgacaca aatctttaat ctcccgtttg atcttcataa tggccatcag
       61 accaccatga cattaagcat tggttacgcg atgaccattg agcacgcctc tgcggaaaaa
      121 ttacaagagc ttgccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg
      181 gtgagataac aaggatatat cgatatgata aagcacctgg tagcacccct gattttcacc
      241 tcactaatac tgactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgcc
      301 gctatgcaat cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat
      361 gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acagatccaa
      421 accatggcag ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc
      481 gataactatg gtgaagacag ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg
      541 gccgatgcat gggctatggg tggacaaatt ccacgcagca atctcaccac acagggttta
      601 ggaaaaaaat atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc
      661 gtcgcagtgg tgattacaac ctcttaaaac acgagcaaac agacaaaaaa agggccagcc
      721 aattggccag cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt
      781 tgatacgagc agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag
      841 caccacgacg tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct
      901 caacgccttc gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac
      961 gaatagcgat aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc
     1021 atactttcac ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct
     1081 cttgttcaag ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga
     1141 tatctcgggg gcttacgcca tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt
     1201 gaactccgcc agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg
     1261 tcttctaagc caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg
     1321 gttgcccgac agtaacactg gcggaacttc catcccttct aacacctcag gccgcgtata
     1381 gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc
     1441 cagtactccc ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc
     1501 accactgaga acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg
     1561 ctcatctata ccttcgtagc gaccgcacac cagaatcaat ttttgattcg ttgccagttc
     1621 gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc
     1681 ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa
     1741 catccccggt ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa
     1801 gtcgcgagga ctccaactct ggatgctcag caggccattt ttaactgccc ggccagttac
     1861 cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat
     1921 agcgccgtct tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat
     1981 tgaacgaata gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg
     2041 ttccttgata ccaaacgcat ctttcaggtt tgccttaatg acgagaacgt cattagatcc
     2101 ggtttccatc atatcgacga ctttaccgag atcgtagcct tcagtggtta ctacctggca
     2161 gcccatcagg tctttccagt agtagtcacc ctcttcaagc tgaggcagct gcgatgaatc
     2221 cacgacaatt tcacaattcg tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag
     2281 cttgatgatc atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg
     2341 acccgccttc tggataaacc agggctgata gtcaaaaatg ctttcggcgt cttcggtgga
     2401 agaaaacact ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat
     2461 gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta
     2521 agctgctttg tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg
     2581 gccaacccag tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc
     2641 gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc
     2701 agcgacaaca acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt
     2761 taccataaca tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg
     2821 tgtcatatta aaagcccgaa aattttactg atttctggag aaaatgcaat cagcagttaa
     2881 taactctgtc gcataaggtc atttagcgac caggaaatcc cggcggcatc atccccttca
     2941 tgcttctcat catcttcgcc attccgcctt tcttcatttt cttcatcatg cgctgcatgt
     3001 cgtcgaactg cttcagaagg cggttcacgt cctgcacctg cataccgcaa ccggcagcga
     3061 tacgacgctt acgcgaacct ttgatgattt ctggcttagc gcgctctttc atcgtcatcg
     3121 agttgatgat ggcttccata cgcaccagca ctttatcgtc catctgcgac ttaacattat
     3181 ccgggatctg ccccatgccc ggcagcttgc ccatcaggct agccatgccg cccatatttt
     3241 tcatctggcg cagttgctca agaaaatcgt tgagatcgaa gccgtcgcct tttttcagct
     3301 tgctggctaa tttctctgcc tgcgcgcggt caactttgct ttcgatatct tcgatcagcg
     3361 acagtacgtc gcccatgccg agaatacgcg acgcgatgcg gtccggatgg aacggctcca
     3421 gcgcctcagt tttctcgcca acaccgagaa acttgatcgg tttgccagtg atgtgacgaa
     3481 tagagagcgc cgcaccgccg cgggcatcgc cgtccacttt ggtcaacact acgccggtaa
     3541 gcggtaacgc ttcattgaat gcttttgccg tattggccgc atcctgaccg gtcatggcgt
     3601 caaccacaaa cagggtttca accgggttaa tcgacgcatg gacttgtttg atctcgtcca
     3661 tcatcgcttc gtcaacgtgc agacgaccag cggtatccac cagcagcacg tcgtagaatt
     3721 tcagtttggc ttctttcagc gccgcgttaa cgatatctac cggcttctga ccaacatcag
     3781 aagggaagaa atcaacgccc acctgctctg ccagcgtctc aagctgtttg attgccgccg
     3841 ggcgataaac gtcggcagaa accaccagca ctttcttctt gtgcttctcg cgcaggaact
     3901 taccgagttt accaacgctg gttgttttac cggcaccttg caggcccgcc atcagtacga
     3961 ccgcaggcgg ttgcgcagcc aggttcaggg tctggttctc ttcgcccatc gccgcaacca
     4021 gttcgttacg gactattttg acgaactcct gccccggcgt caggctctta ttaacttcat
     4081 gaccaaccgc tttctctttt acgcgattga taaactcacg cactaccggc agagcgacgt
     4141 ccgcctccag cagcgccatg cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga
     4201 ggcgtccacg gccactgata ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa
     4261 acattgtctc tcgcctgggg tggaaacggt tggccgcaat cgcgacacat catcagtatt
     4321 ttgccgcagt ataacatgaa ggcgtctttg ttgttatgca acggttggag cagcgttcac
     4381 ctgacgctat actgcttctc tttcttattg ctcaaactgt cgacattact atgcccgttt
     4441 ttgctctgct cgcgcttgtc gcctactccg tcagtcttgc gctgattgtt cccggtctgc
     4501 tgcaaaaaaa cggcggctgg cggcgcatgg ctattatttc tgcggtcatt gcgctggtct
     4561 gccacgcaat cgctctggaa gcccgcatcc tgcccgacgg tgatagcgga caaaacctca
     4621 gcctgctgaa cgttggttca ttggtcagtt tgatgatctg tacggtaatg accattgtgg
     4681 cttctcgcaa tcgtggctgg ttgctgctac ccattgtcta tgcctttgcg cttatcaacc
     4741 tggcgctggc gaccttcatg cccaatgaat acatcaccca tctggaagca acgcctggga
     4801 tgctggtgca cattggctta tcgctctttt cctatgccac gctaattatc gccgccctgt
     4861 acgcgctgca actggcgtgg attgattacc aactgaagaa caagaagctg gcatttaacc
     4921 aggaaatgcc gccattgatg agtatcgagc gtaaaatgtt ccacatcacg cagattggcg
     4981 tggtgctgct aacgctcacg ctttgcactg gcctgttcta catgcacaac ctatttagca
     5041 tggaaaatat cgacaaggct gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc
     5101 tgtggggaca ttaccatgaa ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg
     5161 gcgcggtaat tctgacactg gcctacttcg gcagccgaat tgtccagcag ttaatcagct
     5221 aaacccagaa aaggagtttc ccctggaaca catttctact actacgttga tcattattct
     5281 gatcatcatg gtggtcattt cagcctattt ttccgggtcc gaaaccggaa tgatgaccct
     5341 caaccgctat cgtctgcgac atatggcgaa acagggtaat cgctcggcca aacgcgtcga
     5401 aaaattgctg cgtaagccag accgcctgat aagcctggtg ttaatcggca ataacctggt
     5461 caatattctt gcctccgcgc tcggcactat tgttgggatg cgtttgtacg gcgatgcggg
     5521 cgtggcaatt gcgactggtg tgctgacttt tgtcgtactg gtatttgctg aggtattgcc
     5581 gaaaaccatt gccgcgctgt acccggaaaa agtcgcttat ccgagtagtt ttctgctggc
     5641 tccgctgcaa attttgatga tgccgctggt ctggttgctg aatgctatca cccgtatgct
     5701 gatgcgcatg atgggtatca aaaccgatat cgtggttagc ggctctttga gcaaagaaga
     5761 gttgcgcact atcgtgcacg aatcacgctc acaaatttcc cgtcgcaatc aggatatgct
     5821 gctgtcggtg ctcgatctgg aaaaaatgac cgttgatgac atcatggtgc cgcgcagtga
     5881 aattatcggt attgatatca acgatgactg gaaatcgatt ctgcgccaac tctcccactc
     5941 acctcacggg cgcatcgtgc tctaccgtga ttcgctggac gacgccatca gtatgctgcg
     6001 agtacgtgaa gcctggcggt tgatgtcgga gaaaaaagag ttcaccaaag aaaccatgct
     6061 gcgcgccgcg gacgagatct attttgtgcc ggaaggtacg ccgctcagca cgcagttggt
     6121 aaagtttcag cgcaacaaaa agaaagtcgg cctggtcgtc aacgagtatg gagacattca
     6181 ggggctggtg acggttgaag atattctgga agagattgtc ggcgatttca ctacgtcgat
     6241 gtcgccaaca cttgccgaag aggtcacgcc gcaaaacgac ggttcggtga ttatcgatgg
     6301 caccgccaac gtgcgggaaa tcaacaaagc ctttaactgg catctaccgg aagatgatgc
     6361 ccgcacggtt aatggcgtca ttcttgaggc actggaagag atccctgtcg caggcacccg
     6421 cgtgcgtatt ggcgagtacg atatagatat tctcgacgta caggacaata tgattaagca
     6481 ggtaaaagtt tttcctgtga aaccgctgcg cgagagtgtg gcggagtaac gcaatggcga
     6541 actccggttc gccttttttt tatcgccgtc ttttaggatt ccatcaattc ctgtcattta
     6601 cttagcaatg taataatgtc ttttcaagca ttcggaaagg atattgatgt tatgtcagaa
     6661 gattatgtaa ttgaatggga taaggatttt gcagacgatc tcaacgtcgt cgccagcgtt
     6721 tttttacagc acaccccaac cttatggccc actattttct ctcaactttc gacgcaacct
     6781 gaaatctttg aagacgaaga tgaagacgaa tatggattgc aggacgttct cgattgcagt
     6841 ggtggcgatc tcgggaatcg tgacctggcg caggcatttt tacaggttct acgcggtgaa
     6901 ggattaattc aactcgttga ctggaaaggt gaagatgaag aaggtgagct ggctaatttt
     6961 gctgcagatc gcttttatga gctcacaaaa aacctgatag cttctgagga attaaggagt
     7021 cttcttgtcg aaataaccca agaggatgaa atctccgatg tctgtgaagc cggagaccgc
     7081 taccttgacg aaatctttga acgcatccaa accgaactca ataaaagggg ctttcagatt
     7141 tttgatctga atgaaggatc tgacacctat aacgtggtcg ttctgccaat gaacgaatat
     7201 aaaaaaatag atgatttcaa cacgccgtgg ctggaagtac aggatttttt aagctaaaaa
     7261 aatggccgac ttcgcgtcgg ccatcagata tttacttcgc cttcgctaca gtaaccatcg
     7321 ccgcacgaat cgtacgacca ttcagcgtat aacccttctg cataatgccc agtacgttac
     7381 ctggcgcaac gtcatcagat tccaccattg cgatagcctg atgcacattc gggtccagcg
     7441 gcacgttagt ttcggcgatc acttcaacac caaacttacg cacaacatcc agcatcgact
     7501 tcagcgtcag ctcaatgcct tcaaccatcg cagacatatc cgggttagct ttatcagcca
     7561 cttccagcgc acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca
     7621 gcgcgaattt gtgggctttt tcaatatcca gttcagtacg tcgacgcagg ttttccattt
     7681 cggcttttac acgcaaaatg ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt
     7741 cgagattcgc aattttttca tcgcgcggat ccacctgctc agcagaagct tctggctcaa
     7801 ctgcctcaat ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg
     7861 ttttctgttc tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac
     7921 ttcgcttatt atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat
     7981 cttcgcaaca aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga
     8041 cacccacggc accccactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca
     8101 aaaggttacg aggtcatcgt tgagcaacaa atcgctcacg aactgcaact gaagaatgtg
     8161 aaaactggca cgctcgcgga gattgggcaa ctagctgatc tcgcggtagt cgttggtggc
     8221 gacggtaata tgctgggcgc ggcacgcaca ctcgcccgtt acgatattaa agttattgga
     8281 atcaaccgtg gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacag
     8341 ttagccgatg tgctggaagg ccactacatc agcgagaaac gttttttgct ggaagcgcaa
     8401 gtctgtcagc aagattgtca gaaacgcatc agcaccgcga taaatgaagt ggtgcttcat
     8461 ccaggcaaag tggcgcatat gattgagttc gaagtgtata tcgacgagat ctttgcgttt
     8521 tctcagcgat ctgatggact aattatttcg acgccaacag gctccaccgc ctattccctc
     8581 tctgcaggcg gtcctattct gaccccctct ctggatgcga ttaccctggt gcccatgttc
     8641 ccgcatacgt tgtcagcacg accactggtc ataaacagca gcagcacgat ccgtctgcgt
     8701 ttttcgcatc gccgtaacga cctagaaatc agttgcgaca gccagatagc actgccgatt
     8761 caggaaggtg aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg
     8821 aaagattaca gttatttcaa cacattaagc accaagctcg gctggtcaaa aaaattattc
     8881 taattttacg ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag
     8941 taatggtttt tcatacagga aaacgactat gttggcacaa ctgaccatca gcaactttgc
     9001 tatcgttcgt gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgagac
     9061 cggcgcgggt aaatctattg caatagatgc cctcggtctt tgtctcggtg gtcgcgctga
     9121 agccgacatg gtgcgtaccg gcgctgctcg cgctgacctg tgcgcccgtt tttctctgaa
     9181 agatacgcca gcggctctgc gctggctgga agaaaaccag cttgaagacg ggcatgaatg
     9241 tttgcttcgt cgcgtgatca gcagcgatgg tcgctcccgt ggtttcatca acggtacagc
     9301 tgttcctctg tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca
     9361 cgctcatcag ttactcacca aacccgagca ccaaaagttc ctgcttgatg gctatgccaa
     9421 tgaaacctct ctactccagg aaatgacagc acgttatcag ttgtggcatc aaagctgccg
     9481 tgacctcgcg catcatcaac agttaagtca ggaacgcgct gcccgtgcgg aactgctgca
     9541 ataccaatta aaagaactta acgaatttaa tccgcagccc ggagagtttg aacaaatcga
     9601 cgaagagtac aaacgtctgg cgaacagcgg tcaattgctg accaccagcc agaatgcatt
     9661 ggcattaatg gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca
     9721 actggtgagc gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga
     9781 agaagctacc atccagattg ctgaagccag cgatgaactg cgccactact gcgatcgtct
     9841 ggatctcgat cccaaccgac tatttgaact tgaacagcgc atctcaaaac agatttcgct
     9901 ggcacgtaaa catcacgtca gccctgaggc attgccacag tattaccagt cgctactgga
     9961 agaacagcag caactggacg atcaggccga ctcacaagaa acgcttgcgc tggcggtaac
    10021 gaaacatcat cagcaggcac tggaaatcgc gcgcgcatta caccaacaac gccagcaata
    10081 tgcagaagaa cttgcacagc tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg
    10141 gcagtttacg atcgatgtta aatttgacga gcatcacctg ggcgctgacg gtgccgatcg
    10201 tattgagttt cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagtcgc
    10261 atccggtggt gaattgtccc gcatcgcact ggcaatccag gtcatcacgg cgcgtaaaat
    10321 ggaaaccccg gcactgattt ttgatgaagt ggatgtaggg attagcggtc caacagcggc
    10381 agttgtcggc aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca
    10441 cctgccacaa gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg
    10501 tgcgatgaca gaaacgcata tgcaatccct gaataaaaaa gcgcggttac aagagctggc
    10561 gcgcctgctt ggtggcagtg aagtcacacg taatacactg gcgaatgcga aagaactgct
    10621 tgcagcgtaa acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc
    10681 ggaaagcaaa aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag
    10741 ccacgtactg ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg
    10801 ctgcagcagc agtactattg atgttgaccg caggctgttc cactctggag cgagtggttt
    10861 accgtcctga catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg
    10921 ttggcatgac gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat
    10981 ttggtacgaa tacctggttc tatgtgttcc gccagcaacc aggtcatgaa ggtgtaacgc
    11041 agcaaacgct gacgctgacc tttaacagta gcggtgtgtt gaccaatatt gataacaaac
    11101 ctgcgctgag tggtaactaa taataaagtt gctctcaaag acgttaaaaa aggtgctcaa
    11161 tgagcacctt ttttctgtct gttatttatt cgctgatttt tctgctcgtt gcctgcgaag
    11221 ctctttcgga tcggcaatga gagggcgata aatctccacc cgatcgccat catgcacact
    11281 atcgcttagt tttgccggac ggctgtaaat gccgactttg tttttagtta aatcgatatc
    11341 ggtacgcaat tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg
    11401 cagcgtcacg cgctgcaggt actgcttctc aggtagcgca taagccacct caacggcaat
    11461 tttacctggc actgtaaacc tctttcgcac gaaccgtaaa agcctggacc atattagacg
    11521 ccagctcttt aaacacgcga ccaaaggcga gttcaatcaa cttattggta aactcaaagt
    11581 cgagatgaaa ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc catccaccaa
    11641 tcaatttctt gaacggccca tccaccagat tcataagaat actttggtta ctggtcaact
    11701 ggttgcgggt agtaaacgtt ttgctgatcc cagccttaga gacatctacc gccgcagtca
    11761 tctgcccagg agtggactcc agaatccgac ttccggtaca acccggcaaa aactgaggat
    11821 aagactgaac gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg
    11881 tccggctaat ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt
    11941 atcctgctgt aaaaaaaacg ctatcccggc gctgggtaac atcgggttca tgctaagata
    12001 gagccttgtc ccccgcagga ttgatatggg gtgttttcga tttcagatta ccgatgattc
    12061 acgacgctta tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac
    12121 aagcgcgccc gtcacgaata ctttatcgaa gaagagttcg aagcgggact tgccctgcaa
    12181 ggctgggagg ttaaatccct gcgcgcagga aaagccaata tcagcgacag ctacgtcctt
    12241 ctgcgtgacg gagaggcatt tctgtttggc gctaacatca cgccaatggc cgtggcctcc
    12301 acgcatgtgg tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca acgcgaactg
    12361 gactcattgt acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac
    12421 tggaaaaatg cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaaaaa acagcacgat
    12481 aaacgttcag atatcaaaga acgcgaatgg caggtggata aagcacgtat catgaaaaac
    12541 gcccaccgtt aaacctgcac tccaattatt gaccagttcc tcaccgcgcc tccctctccg
    12601 gcggcgcgaa tgaacatctt attggctatc acatccgaca caaatgttgc catcccattg
    12661 cttaatcgaa taaaaatcag gctacatggg tgctaaatct ttaacgataa cgccattgag
    12721 gctggtcatg gcgctcataa atctggtata cttaccttta cacattgggg ctgattctgg
    12781 attcgacggg atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag
    12841 ccgcaaaaaa tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag
    12901 agccctctct ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag
    12961 agatcgcgtg gaagccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt
    13021 agtggcgtgt ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc
    13081 gaggatgtag gaatttcgga cgcgggttca actcccgcca gctccaccac tttaagaagg
    13141 actacaaccg gacagtagca ataaatacag ccacttacgg acacaagcca gacagtagtc
    13201 ggactgaaaa aagacaaaaa tatacacgtg aaatgcacgt gcacttctaa aaaaccccag
    13261 ttatcaccga ctggggtttt tcaatttgta actaagggta acaaaaacac ctccgttttt
    13321 tttcatctca ctcaccttga cactgtttat ttgtacagtt aaaaataata ctgtatacaa
    13381 acacagtata gagggacttt tatgcgtatt gagatctgca tagccaaaga gaaaatgact
    13441 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taactcggcg catcagtaag
    13501 cgttatgacg atgtagaagt gatcgtaaaa gccaccagca atgatgggct ttctgttaca
    13561 cgcacagcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
    13621 gagtctgctg acgagtggtt tgttcactaa taaacatgta aaatcggtaa cggctggaaa
    13681 tcattcaata ctcgcactat cgaaaagctt accagccaac cgcggcaagt tgcatacaac
    13741 gtgccgcgtt ttccagatca gctcgttaat ggagacatta ttgccggaga taatggtgga
    13801 ttcagcatcc agagtaaccc cggcggcacc gtatcaaaag gacgcccaag atcatcaaca
    13861 cattttttta atctgaaatc tgtaatgctc tctccattcc ctaggcattg accgcttcct
    13921 attgacttga tttgcacagc cccagtatca ctggttataa atgtaaacaa tgcgttacgg
    13981 tcgctttcag agcactgctt agccactgcc aattgcccgt ttagaatagc aaagcattca
    14041 ttgccatcag gatttttaat ctgaacatgc cctcccggtg ccgatatcaa atccaaatca
    14101 ttagtcttta cttcagatat aacccaatta tttacattaa agtcactacc aacaggattt
    14161 cggattatcc cccctgtaag aacgtttctg atattaacca atactggact gggttcagga
    14221 tctacagggg cattattgcc gacaaactga gtaagaagat tacgctgatt tgcaggttca
    14281 gaagcacaac ctgtcagcag taaaacaaaa agcacaataa ctgttttcat cttctcgctc
    14341 ctcttccagg aataaagcca actggaaagt gatcagaggc aatttgccct cttaataatc
    14401 cgaataacaa cgaggccctc aacagaggaa gtacaaagct cactgaattt ccaactaccg
    14461 cataatcaag aacaccacca ctggcttgcg ttggatctgg tggagcaata at
//
LOCUS       DAHBHC010000108        14050 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHBHC010000108 DAHBHC010000000
VERSION     DAHBHC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.109"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(33..329)
                     /locus_tag="M2A35_004356"
     CDS             complement(33..329)
                     /locus_tag="M2A35_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCB7200573.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(543..1829)
                     /gene="thrC"
                     /locus_tag="M2A35_004357"
     CDS             complement(543..1829)
                     /gene="thrC"
                     /locus_tag="M2A35_004357"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCB7200574.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1830..2762)
                     /gene="thrB"
                     /locus_tag="M2A35_004358"
     CDS             complement(1830..2762)
                     /gene="thrB"
                     /locus_tag="M2A35_004358"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCB7200575.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2764..5226)
                     /gene="thrA"
                     /locus_tag="M2A35_004359"
     CDS             complement(2764..5226)
                     /gene="thrA"
                     /locus_tag="M2A35_004359"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCB7200576.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLGNWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5252..5371)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5307..5372)
                     /gene="thrL"
                     /locus_tag="M2A35_004360"
     CDS             complement(5307..5372)
                     /gene="thrL"
                     /locus_tag="M2A35_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCB7200577.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5586..6272)
                     /locus_tag="M2A35_004361"
     CDS             complement(5586..6272)
                     /locus_tag="M2A35_004361"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCB7200578.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6672..6812)
                     /locus_tag="M2A35_004362"
     CDS             complement(6672..6812)
                     /locus_tag="M2A35_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200579.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6908..7624
                     /gene="arcA"
                     /locus_tag="M2A35_004363"
     CDS             6908..7624
                     /gene="arcA"
                     /locus_tag="M2A35_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCB7200580.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7684..9036)
                     /gene="creD"
                     /locus_tag="M2A35_004364"
     CDS             complement(7684..9036)
                     /gene="creD"
                     /locus_tag="M2A35_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCB7200581.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9094..10518)
                     /gene="creC"
                     /locus_tag="M2A35_004365"
     CDS             complement(9094..10518)
                     /gene="creC"
                     /locus_tag="M2A35_004365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCB7200582.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10518..11207)
                     /gene="creB"
                     /locus_tag="M2A35_004366"
     CDS             complement(10518..11207)
                     /gene="creB"
                     /locus_tag="M2A35_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCB7200583.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11220..11693)
                     /gene="creA"
                     /locus_tag="M2A35_004367"
     CDS             complement(11220..11693)
                     /gene="creA"
                     /locus_tag="M2A35_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCB7200584.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11904..12773
                     /gene="robA"
                     /locus_tag="M2A35_004368"
     CDS             11904..12773
                     /gene="robA"
                     /locus_tag="M2A35_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCB7200585.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="M2A35_004369"
     CDS             complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="M2A35_004369"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCB7200586.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13469..13990
                     /gene="yjjX"
                     /locus_tag="M2A35_004370"
     CDS             13469..13990
                     /gene="yjjX"
                     /locus_tag="M2A35_004370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCB7200587.1"
                     /translation="MHQVVCATTNPAKIQSILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         3441 a         3822 c         3402 g         3385 t
ORIGIN      
        1 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
       61 ccgccgtgat gatcatgatg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
      121 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
      181 tgaccgccat cccagtaata accgcgatta tcacgatcgc ctatctgtaa ttttactgac
      241 gggactaacg taatttccgc agcctgcgct gccatgggag cgaccagaac cagggaaagt
      301 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
      361 gtcgatatcc ggagattacg cgatgagaac ggcggttgtt attctccgca atcctaatct
      421 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
      481 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
      541 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
      601 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
      661 agaatcgatt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
      721 tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc
      781 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
      841 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
      901 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
      961 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
     1021 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
     1081 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgtgg cagctgcgca
     1141 acagcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagct
     1201 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
     1261 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
     1321 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
     1381 ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
     1441 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
     1501 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
     1561 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
     1621 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
     1681 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
     1741 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
     1801 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
     1861 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
     1921 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
     1981 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
     2041 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
     2101 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
     2161 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc cgtcgagact
     2221 ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
     2281 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
     2341 tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc cagcaaacga
     2401 gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
     2461 caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt catcgccact
     2521 ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
     2581 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
     2641 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt caccgccgcc
     2701 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
     2761 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
     2821 tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
     2881 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc
     2941 cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa catagcgcaa
     3001 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
     3061 cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg gcagcacagg
     3121 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaatcaatag
     3181 cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat
     3241 ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
     3301 ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag
     3361 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
     3421 tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca tcgacgaggt
     3481 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt cggcatattg
     3541 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
     3601 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc
     3661 ctgccagttt cccagattta ggccatgcac attggtgagc agtgccttcg agttggcaac
     3721 accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac gcttcagttg
     3781 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
     3841 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
     3901 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
     3961 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
     4021 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
     4081 cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgtgg
     4141 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
     4201 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
     4261 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
     4321 gtcttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
     4381 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
     4441 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
     4501 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
     4561 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
     4621 aagtaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca acaccatgtg
     4681 atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
     4741 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
     4801 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
     4861 cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat gcaggacatg
     4921 ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg ggaaccccgg
     4981 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
     5041 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
     5101 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
     5161 atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
     5221 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
     5281 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg
     5341 gtggtggtaa tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc
     5401 tgtctgtgcg ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt
     5461 aatttactca cggcaggtaa ccagttcaga agctgctatc agacactctt tttttaatcc
     5521 acacagagac atattgcccg ttgcagtcag aatgaaaagc tgaaaaatac ttactaaggc
     5581 gttttttatt tggtgatatt tttttcaata tcatgcagca aacggtgcaa cattgccgtg
     5641 tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt cgaccagttt tatgtcatct
     5701 gccactgcca gagtcgtcag caatgccatg actcgttcgc gtaaagcttg cagttgatgt
     5761 tggtctgccg ttgtgtcact tttcgccggt tgttgtatta atgttgctaa ttgatagcaa
     5821 tagaccatca ccgcctgccc cagattgagc gaaggataat ccgccaccat cggcacacca
     5881 gtaagaacgt cagccaacgc taactcttcg ttagtcagcc cggaatcttc gcgaccaaac
     5941 accagcgcgg catggctcat ccatgaagat ttttcctcta acagcggcac cagttctact
     6001 ggcgtggcgt agtaatgata tttcgcccga ctgcgcgcag tggtggcgac agtgaaatcg
     6061 acatcgtgta acgattcaac cagtgtcggg aaaactttaa tattatcaat aatatcacca
     6121 gatccatgtg cgacccagcg ggtggctggc tccagatgtg cctgactatc gacaatccgc
     6181 agttcgctaa aacccatcgt tttcattgcc cgcgccgctg ccccaatatt ttctgctctg
     6241 gcgggtgcga ccagaataat cgttatacgc atattgccac tcttcttgac caaataaccg
     6301 cgaacagggt gatcactgtc aacttattac gcggtgcgaa tttacaaatt cttaacgtaa
     6361 gtcgcagaaa aagcccttta cttagcttaa aaaaggctaa actatttcct gactgtacta
     6421 acggttgaat tgttaaaaaa tgctacatat ccttctgttt acttaggata attttataaa
     6481 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
     6541 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
     6601 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
     6661 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
     6721 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
     6781 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
     6841 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
     6901 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
     6961 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
     7021 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
     7081 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
     7141 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
     7201 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
     7261 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
     7321 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
     7381 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
     7441 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccacacga
     7501 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
     7561 gccggaaatc atcgccacca tccacggtga aggttatcgc ttctgtggtg atctggaaga
     7621 ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     7681 accttatttc cagatacgta attgatcgtc cattgtaact tctttactgg ctttcatttt
     7741 cggcagtgaa aacgcatacc agtcgatatt acgagtcaca aacatcatgc cggccagcgc
     7801 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     7861 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     7921 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     7981 tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa acattaccaa
     8041 tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     8101 aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag tcgcccggtc
     8161 cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     8221 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
     8281 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
     8341 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     8401 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     8461 cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     8521 ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca ccacaccaat
     8581 accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga tatttggcgc
     8641 attgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     8701 gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc catcaaccat
     8761 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcctc
     8821 ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa cgagtttttg
     8881 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     8941 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
     9001 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     9061 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     9121 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     9181 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     9241 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     9301 atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatacgacc
     9361 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa
     9421 cgccggttca gcagcaacat taacctcggt gggcgtaaca tgtagagtga tgtttttttc
     9481 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
     9541 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
     9601 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ctacttcagg
     9661 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     9721 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     9781 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg
     9841 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
     9901 gatccaccaa accatgcctg cgccaatcac cagtgcgatc cccaacaaaa tggcgctggc
     9961 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
    10021 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    10081 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
    10141 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    10201 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    10261 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    10321 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    10381 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    10441 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    10501 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    10561 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    10621 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
    10681 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    10741 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    10801 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    10861 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    10921 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    10981 aacgccggat ggagcgccag taactggcgg cacaattcaa agccactgat atcaggcaaa
    11041 ccaacgtcaa gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaaa
    11101 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
    11161 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    11221 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    11281 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    11341 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    11401 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc
    11461 gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt tttcgcccgg
    11521 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    11581 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    11641 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    11701 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    11761 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    11821 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
    11881 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    11941 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    12001 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    12061 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    12121 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    12181 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    12241 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    12301 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    12361 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    12421 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    12481 aacaagaggt attctatacc actgcgttag cccaggatca ggcagatggc tatgtactga
    12541 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    12601 gaaccggcgt gcaggagttt atcctgacag tatacggaac gtgcatgcca atgctcaatc
    12661 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
    12721 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
    12781 actcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acaacccagc
    12841 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    12901 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    12961 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    13021 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
    13081 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    13141 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    13201 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
    13261 tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    13321 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    13381 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    13441 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
    13501 cgctaaaatt cagtccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    13561 tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca gtgaggaaac
    13621 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    13681 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    13741 aaacgccagc cagcgcggcg aggcgcgttc ggctactttg ccgttgcctg cggtgattct
    13801 ggaaaaagtg cgtgaaggtg aggcacttgg tcccgtgatg tcgcgttata ccggtattga
    13861 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    13921 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcataatg cggtgtatca
    13981 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    14041 tgcgacgctg
//
LOCUS       DAHBHC010000109        13268 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHBHC010000109 DAHBHC010000000
VERSION     DAHBHC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.110"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..438)
                     /locus_tag="M2A35_004371"
     CDS             complement(<1..438)
                     /locus_tag="M2A35_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCB7200588.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIAR"
     gene            complement(556..1281)
                     /gene="gltL"
                     /locus_tag="M2A35_004372"
     CDS             complement(556..1281)
                     /gene="gltL"
                     /locus_tag="M2A35_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCB7200589.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(1281..1955)
                     /gene="gltK"
                     /locus_tag="M2A35_004373"
     CDS             complement(1281..1955)
                     /gene="gltK"
                     /locus_tag="M2A35_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCB7200590.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(1955..2695)
                     /gene="gltJ"
                     /locus_tag="M2A35_004374"
     CDS             complement(1955..2695)
                     /gene="gltJ"
                     /locus_tag="M2A35_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCB7200591.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(2865..3773)
                     /gene="gltI"
                     /locus_tag="M2A35_004375"
     CDS             complement(2865..3773)
                     /gene="gltI"
                     /locus_tag="M2A35_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCB7200592.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            4228..6105
                     /locus_tag="M2A35_004376"
     CDS             4228..6105
                     /locus_tag="M2A35_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCB7200593.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(6183..7721)
                     /gene="lnt"
                     /locus_tag="M2A35_004377"
     CDS             complement(6183..7721)
                     /gene="lnt"
                     /locus_tag="M2A35_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCB7200594.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYKSADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7746..8624)
                     /gene="corC"
                     /locus_tag="M2A35_004378"
     CDS             complement(7746..8624)
                     /gene="corC"
                     /locus_tag="M2A35_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCB7200595.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(8714..9181)
                     /gene="ybeY"
                     /locus_tag="M2A35_004379"
     CDS             complement(8714..9181)
                     /gene="ybeY"
                     /locus_tag="M2A35_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCB7200596.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(9178..10218)
                     /locus_tag="M2A35_004380"
     CDS             complement(9178..10218)
                     /locus_tag="M2A35_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCB7200597.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(10371..11795)
                     /gene="miaB"
                     /locus_tag="M2A35_004381"
     CDS             complement(10371..11795)
                     /gene="miaB"
                     /locus_tag="M2A35_004381"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCB7200598.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDINFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            11941..13116
                     /gene="ubiF"
                     /locus_tag="M2A35_004382"
     CDS             11941..13116
                     /gene="ubiF"
                     /locus_tag="M2A35_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCB7200599.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3161 a         3396 c         3510 g         3201 t
ORIGIN      
        1 acgggcaatt ttgctatgca gttccggatg gctattgagc agcaaggcaa cgttagtttg
       61 cggtccggta gacacaatcg tgacaggttc cgcactttca cgcagcgttt tcgccattag
      121 ctctaccgcc gtacagtttt gcggtgcgaa cgccggttcc ggtaatgccg ggccgtcgag
      181 accgctttcg ccgtgcacat tgtccgcgat aatcaactca cgcattaacg gttttaccgc
      241 gccgcctgct accggaatat cggtgcgatt gagcaaggtc agcatacgaa gaacattgcg
      301 taaggttttt tctggtgtct ggtttccggc ggaagacgta attgctttga catcaagctc
      361 tggtgaggcg agggcgagaa ctattgcgat agcgtcgtca tgacctgggt cgcaatctaa
      421 cagaattggc agtgccattg ttgctccttg ttgtgtgctt ctttgcgaca agggtaacgc
      481 caggatgtaa cagatacgag gggcgaaacg ataaagcgtg agatggcgcg cacttgggta
      541 tgcgcgccag agtgattaat gcaggatttt cgcgaggaag tcttttgcgc ggtccgattt
      601 cggatcatcg aagaaagcgt ctttcggcga gtcttcgaca attttaccct catccataaa
      661 gatcacccga ttcgccactt tgcgggcaaa gcccatttcg tgggtcacca ccatcatggt
      721 cattccttcg ttcgccagtt ccaccatcac gtccagcact tcgttgatca tctccggatc
      781 cagcgccgac gtcggttcgt cgaacagcat cgcaatagga tccatacaca atgcgcgagc
      841 gatcgccaca cgctgctgct gaccgccgga aagctgcgcc ggaaatttat tggcatgtgc
      901 ggaaagtccc acgcgctcca gcaatttcaa tgctttttca cgagccggcg ctttatcgcg
      961 tttaagcact ttcacctgcg ccagggtcag gttttcgata atcgacagat gagggaacaa
     1021 ctcaaaatgc tggaacacca tcccgacgcg ggaacgcagc tttgccagat cggttttctt
     1081 gtcattaacc acgataccat cgacggtgat ttcaccttgc tgcactggtt cgaggccgtt
     1141 gacggtttta atcagcgttg atttgccgga gccagacggg ccgcaaacca ccaccacttc
     1201 gccttttttc acttcggttg agcagtcggt cagcacctga aagtgaccat accattttga
     1261 aacatttttc agggtaatca ttatgctgtc cttcttttca agtagctgac caacaacgac
     1321 gcgctaagac taataacgaa ataaacaaat ccggcaaaca ggatcatctc aacctgcgta
     1381 ccatcacgct caccaatggt tgaggcggta cggaagaaat cggccagaga taacacatac
     1441 accagtgagg tatcctggaa cagtacgatg ccctgagtga gcagaagcgg caccatcgcg
     1501 cggaacgcct gcggcagaat aatcagtttc atcgactgcc agtgagtcat tcccaacgcc
     1561 agcgcggcgc tcgactgacc acgagaaata ctttgaatac cagcacggat aatctctgaa
     1621 tagtaggccg cttcaaacat cgaaaacgcc accatcgccg aaattaaacg gatatcattt
     1681 tttggcgata atcccagcac gttttgcaga aaacccggca cgatcaggta aaaccacagc
     1741 aaaaccataa ctaaaggaat cgagcggaat acgttaacgt aggctttggc aaaccacgct
     1801 acgggcgcaa agctggacaa gcgcatcacc gccagcatgg tgccccacaa aataccaatc
     1861 acgactgccg tgacggtgat tttcagcgtg atcaccagcc cgtcgagcag atatggcagg
     1921 gaaggaacaa tggaactcca gtcaaactcg tacattattt gccccccatg ttgccgggca
     1981 ggcgaacttt acgttcaacc agcgtcatca ccagcatgat aaccgcgtta atcaatacat
     2041 acgccagcgt aatggcggta aacgactccc aggcgtgagc cgagtagtcg agcaatttac
     2101 ccgcctgcgc tgccatatcc accagaccga tagtcgaggc gatggcggag tttttcacca
     2161 ggttcatcat ctcggaggtc atcggcggga cgataacgcg ataagcatta ggcagcagta
     2221 catagcggta agcctgcggt agcgtcaggc ccatcgccag cgcggcattc ttctgcccac
     2281 gcgggagcga ctgaatagcg gcgcgtacct gttcgcaaac acgggcggcg gtaaacagcc
     2341 ccaggcagag catggatgaa aggaaaaact gaatattggg atccagctcg gccttaaacc
     2401 acatgccgat tttctccggc aacagctccg ggatcaccag ataccaggta aagaattgca
     2461 caatcaacgg cacgttgcgg aacagttcga catacaacgt acccagaccg gagagaaaac
     2521 ggtttggaac ggtacgtaaa atgccgaaaa atgaaccgac gaggaaagcg ataatccagg
     2581 cgcagatcga caaagcgatc gtcacctgaa aaccactcca gatccaacca agataggtgg
     2641 tgttgccgaa cggggcttgt tgtaaaaaaa taccccagtt ccagtctata gacataaatc
     2701 tactccagaa aaaagagggt agcagcgtta actgctaccc tcgaagattg ttacccagcg
     2761 tattgcggtt ttcaggcccg atggggaacg accatcaggc gtatagtctg tccgtgctac
     2821 gtaacaatcg agagggctgg aatttccgcc cctggttctt gtaattagtt cagtgccttg
     2881 tcattcggtt ctttgaacag tgctttcatt tcgtctgaca gttcgaaatt catgttcagg
     2941 tttttcggcg gaattggatt tttgaaccat ttatcaaacc atttttccgc ttcaccggag
     3001 gtctgcacct gagcgatggt gtcatccatc agctttttga actgcggatc atctttacgc
     3061 aacatacaac cgtaggcctc ctgagactgc ggcttgccga cgatttccca gttgtctggt
     3121 ttcttcgctt tcgcacgttc accggccagc agggcatcat ccatcataaa ggcaacggca
     3181 cgaccgcttt ccagggtgcg gaaagagtca ccgtgatctt tggcgctgat gatgcgcata
     3241 ttcatttttt gctcttcatt cagtttgttg agcaaaactt cagaggtagt gccggaagtg
     3301 acgactacgg ctttgtcttt caggtcggca aaatctttga tatcgccgcc ctttttggtc
     3361 agcaggcgcg taccgaccac gaaaatagtg tcagagaaag ccgcctgttt ttggcgttcg
     3421 acgttgttgg tggtagaacc acattcaaaa tcaaaagtgc cgttttgcag cagcggaata
     3481 cggttttgtg aggtaatcgg aatcagtttt acctgcaagt ccggtttgtt gagtttcttt
     3541 ttcactgctt caacaatggc gttggagtaa tcctgcgagt aacccaccac tttttgctga
     3601 ttgtcgtaat aagagaaagg tactgaagat tcacgatgac cgacgacaat cacaccgttt
     3661 ttggcgattt tgtccagcgt actgcccgct accggggcgg cgtcatctgc ctgtgccagt
     3721 cctgcggaaa gcgccagggc gaggattgct gtggcaggtt tacgtaattg catatccaac
     3781 tcctttatct tctgcgttaa gaacgcatgg atacccgttg tgagtgtttg tgttgttatc
     3841 gtctgcaact ttattgtgca gtgttgtgcc tgttaggtaa catttagttt ggctaaatgt
     3901 aaagatattg ctgttttatt gtttgttttt gcgagatgcg ccgcaccatt ccgaagcaaa
     3961 attcttaaac cgcacctttt tggtgctacc gctgatttgt ggtggtgcaa cctggttgcg
     4021 tcgcggctga tttcatcgct tacacagtaa ataaagcaac ttacatgcca aaaaatactt
     4081 aggggcgaat taattacgat tgtaatttta ggctgaaaaa gtcatatttc ggacagaatt
     4141 atcttttcgg catgggacgt ttgccgggat gataactgca cacgggaaaa atgcttttta
     4201 cctgtctttt caaggaagaa acaccttatg tctgcatctt cggttaagcc gttaaatgtt
     4261 caattacccg caataaccct tatccttttt gcactctgta ttgggatatt ttgttatctc
     4321 gcacaatgga tgagttatga agaggtcgat caatccgcac ttatccatct cggcgcaaac
     4381 gttgcgcctc ttactttgtc gggtgaaccc tggcgcttat tgagcagtat ctttctgcac
     4441 agtagtgttt ctcatctgct gatgaatatg tttgcattcc tggtcgtggg aggcgtggcg
     4501 gaacagattc tggggaaatg gcgactcctg attacctggt tattctccgg cgtctttggt
     4561 gggctcatca gcgcctgtta tgcgttacgc gaaagtgagc agatagtcat cagcgttggg
     4621 gcatccgggg cgattctggg aatagctggt gcagcgatag caacacagtt tgcttcaggt
     4681 acgggcacat accataaaaa ccagcggcga gtatttcctc tgttgggtat ggtggcgctg
     4741 acactgttgt acggtgcccg gcaaacagga atagataacg cttgccacat tggcggcctg
     4801 attgcgggtg gcgcgttggg ttggctgagc gcgcgtttat ctgggcaaaa ccgactcgtt
     4861 acggaaggcg ggattattgt tgcgggcagt cttcttctga ccggggctat ctggcttgcg
     4921 cagcagcaga tggatgagtc agttttacaa gtcaggcaaa gcctgcgtga agcgttttat
     4981 ccacaggaga ttgaacaaga gcgacggcaa aaaaagcagc agttagcgga ggaacgcaac
     5041 gccctcaggg aaacattatc cgctccggta agtcgtgaac aggccagtgg tgatttgctc
     5101 gctgagattg ccgatatcca tgatatggcg atcagtcggg atggtaatac gttgtatgcc
     5161 gcaattgaag acaccaacag cattgttgtt ttcgacctcg gacaaaagaa aatcctgcat
     5221 acctttacag cgcccatagc gaaagaaaag tcagtcaaac attgtggtgg ctgtaaagat
     5281 cagggcgtca gatcgctggc attaagcccg gatgaaaagt tgatttatgc gacttcattt
     5341 gaagcgaatg cgttatcggt cattaacgtg gcgacagggg agattattca gtcgattacc
     5401 accggtgcac atcctgacag tcttatcctc tcgcgtgatg gcacaaaagc ctgggtgatg
     5461 aatcgcacca gtaatagtgt gtcagccatt gatctggtgg cttatcagca tgtggcggat
     5521 atcccgctgg agaaatacga cgggacgggg acgagcggta aaccaggcgc ctgggttatg
     5581 gcactttccc cggatgaaag aacattactg gttccgggag aaggcagagg taacatcgtg
     5641 cggatcaata ccatcacgca tcagaaagaa gactttcccg caggtgatgc gcgtggaacg
     5701 atatcggcga tgcgttttcg acctgaaaac ggcgaggtta tttttgccga tagtcagggg
     5761 atttcacgta taagcatagg ggatcaacaa accagcatta tgacgcaatg gtgtagcagg
     5821 agcgtttatt ccgttgaggg tattagcccg gacggtcaat atttagcgtt ggtgtcatat
     5881 ggactgcaag gttatgtcat cctgctcaat attaatgccg ggcagattat tggcgtttat
     5941 cctgccagct acgttaatca ccttcgtttt tcagcggatg gtagaaaaat atttgtcatg
     6001 gcgaagaacg ggttgatcca aatggacagg acgcgctcgc ttgatccgca ggcaattatt
     6061 cgtcatcccc aatatggcaa tgtggcttgt atccctgaac cgtaagaaaa aggggcatga
     6121 taaccatgcc ccaaagctta atgagaaagt acgttttgct catgcaggat acatcaggca
     6181 catcaacgtt tacgtgctcg cagactcatc agcacagcgg caaaaccaaa cagcgccgtc
     6241 agcacccaca gcggccagtt gccggtacgt gcatacggtg tgagtccggt ggtcggtgtc
     6301 acattagtgg tcaacacctc gcgggtgaac tgcgggatca tcgcctgaat ctcaccctgc
     6361 gggccaatca ccgccgtaat gccgttgttg gtgctgcgca acagtgggcg cgccagctcc
     6421 agcgcacgca ttcgcgccat ctggaagtgc tgccatggac caatagactt accaaaccac
     6481 gcatcgttgg agatagtcag cagatagtcg gtatccgggc ggaagttatc gcgcacttgc
     6541 tcgccgagaa tgatctcgta gcaaatagcc gcagtaagct caataccatt tgccgacagc
     6601 ggcggctgga tatatggccc acggctgaac gacgacatcg gcagatcaaa gaacggtgct
     6661 aacggacgca gaatcgactc cagcgggaca aactcgccaa acggcaccag atggtttttg
     6721 ttatagcgat cggctgattt gtagctgtac ggtgcacctt tacccagcgt gatgatggtg
     6781 ttgtaggtat cgtagcggtt ctgcttattg agacgcgcgt cgacaatccc ggttaccagc
     6841 gagctacctt tatcgcgcaa ctcaccgtcc agtgctttga ggaacggttg ctgattaatt
     6901 tccagatcgg ttatcgccga ctccggccag ataatcaacg atgatttgcc catcagcgga
     6961 gccgttgcgt tgtagtaaat cttcagcgta ttaagaagct gatctccatc ccatttcagt
     7021 gattgcggaa tatcgccctg aaccatcgaa acctgaatgg ttttctccgg ttgtggggta
     7081 aaccattgga tgtaacgcag cgggaagggt agggcaaaca gcacgacggc caccaccagc
     7141 ggacgccagt tgcgtttgac caacgccagt gccagcaggc cactaaccat catcagcagg
     7201 aagttaatgg cttccacgcc cattatcggt gccagccctt ttaacgggcc atcaatctgg
     7261 ctatagccga actgtaacca cgggaagcct gtcagtaccc aaccgcgcag aaactcggtc
     7321 acttgccaga gagaaggggc ggcaatcgcc acgcgcagcc aggtggtttt cggccacaga
     7381 cgcgacagca cgccagcaaa cagtccggta tacagcgaca aatacgccgc cagcagcacc
     7441 accaggaaga tgttaaccgg gccaggcatc ccgccaaagg tcgcgatgct gacatagacc
     7501 cagttaatac cgctgccaaa gaggccaaat ccccagcaaa agccaatagc ggcagactgg
     7561 agtggacggc ggttaaaggt caacgcctga agccccatca gcgaaataat cgccgcaggc
     7621 cagacatcgt aaggagagaa ggccagcgtt ccgcaggcac cgaataataa cgccagcagc
     7681 aggcgaatgc gctggcgttc aattaatgag gcaaaagcca tgtagttatc tatccagttt
     7741 cggttttatt catccagctt cggctggggt gagtcatccg ggattttgac atgaacctga
     7801 ataatacgcc gactgtcggc catcgccact ttgaactggt aaccgtcgat gtcgatagtt
     7861 tcgccacgcg ccggaagatg cccaaatgcc tgcatcacca gaccaccgat agtgtcaacc
     7921 tcttcatcgc taaagtgggt gccgaacgct tcgttgaagt cttcaatgga agccagtgcg
     7981 cgcacggtcc aggtatgacg actcagctga cggaagtcga tatcatcttc ttcgtcatac
     8041 tcgtcttcaa tctcaccaac aattagttcc aggatgtctt caatggtcac cagaccggaa
     8101 accccaccga attcgtcaat aacgatcgcc atgtggtaac gctgagagcg aaactctttc
     8161 agcatccggt ctacgcgctt actttcagga acgacaaccg cctgacgtaa cactttgtcc
     8221 atgctgaagg cttcagcatc gctgcgcata aacggcagca agtctttcgc catcagaatc
     8281 ccttcaatgt gatctttgtc ttcgctaatc accgggaaac gtgagtgggc ggactcgatg
     8341 atgacatcaa ggcattcgtc cagcgtctgg ttgcgtttca gggtaatcat ctgggagcgg
     8401 gggatcatga tgtcgcgaac gcgttggtct gcgatgtcca tcaccccttc gagcatatcg
     8461 cgcgtatctt cgtcgataag gtcgttctgc ccggaatcac ggatcagcgc cagcagttcg
     8521 tcacggtttt tcggttcacc gtggaaaagt tggctgagta acagggagaa aaatcccttc
     8581 ttgttgctta tcgtgtcact actgtgtgaa ttgtcgtcgc tcatggcgtc gtatgggttc
     8641 tcatgttagt taatcaaaac gccgtcgtta atcaccaacg gcggggacgt ctgccagtca
     8701 aatgcctggc aaattattct ttctcggcaa tgtacggatc ctcatagccc agagcaagca
     8761 taatctctgt ttcgagggct tccatttctt ctgcttcgtc atcttcgatg tgatcgtaac
     8821 ctaacaaatg cagactgccg tgcaccacca tatgcgccca gtgcgcctcc agtggtttgc
     8881 cttgctcctg agcttccttc tcaaccacct gacggcagat aaccagatcg cccagtagcg
     8941 acatttccat gccaggcggc acttcaaacg ggaaggagag cacgttggtc ggcttatcct
     9001 taccgcgata ggtcagattc agactgtggc tttcggcggt atcgaccacg cgaatagtca
     9061 cttccgattc ttcctgaaac tgcgggatca ccgcattcag ccatgtttga aactggctct
     9121 cttccggtaa cccagaatta tcttcacatg ccagttgtaa atcgaggatc acctgactca
     9181 tttttgttcc tgttcttcgc gcttgcgttc tgccgccagc gccgcttttc gtttttgttc
     9241 ggcttcttcc caggcttcat aggcgttaac gatacgcgcc accaccgggt gacgaaccac
     9301 gtcttcgctg tggaagaagt taaagctgat ctcttcgaca tcggccagca cttcgatggc
     9361 gtgacgtaag cctgatttag tattacgcgg caggtcgatc tgtgtgacgt cgccggtgat
     9421 aaccgctttt gagttaaaac cgatacgggt caggaacatc ttcatctgtt cgatggtggt
     9481 gttctggctc tcatcgagaa tgataaacgc gtcgttcagc gtacgaccac gcatataggc
     9541 cagcggcgcg acttcaataa cgttgcgctc aatcagtttc tcaactttct caaagcccag
     9601 catttcaaac agcgcgtcgt acagcgggcg cagatacggg tctactttct ggcttaaatc
     9661 gccaggcagg aagcccagtt tctcaccggc ttcgaccgcc ggacgagtca gcagaatacg
     9721 gcgaatctcc tgacgctcca gggcatcaac tgccgcagcc actgccaggt aggttttacc
     9781 cgtacccgcc gggccaacgc cgaaggtaat gtcatggtcg agaatattgg cgatgtactg
     9841 cgcctggttt ggcgtgcgcg gcttaattac gccgcgtttg gttttgatat tgaccgcttt
     9901 gccgtactcc ggcacgctct ccgcactttg ctccagtact cgcgcttctt taatcgcaag
     9961 gtggatctgt tccggttcga tatcctgaat ctgaccgcgc atcggggctg tatcgacata
    10021 caggctacgc agaatgtctg ccgcagcggt gacgcaaatc ggacggccgg tcagtttaaa
    10081 gtggttgtcg cggcgattga tctcgatgcc gagacggcgt tcgagctgct tgatgttgtc
    10141 atcaaacggg ccgcacaggc tcaacagacg cgcattgtct gctggctcca gggtgatttc
    10201 gcgagtgtct atgttcaaac cgttcctctt atctgtatgc cgccggaagc tgaacattca
    10261 ccggcctata aggaaattat tcacgccaca ggaaaaaggc gcaagcattg cgataaagat
    10321 ggggataaag agagaaaaaa caaggcccac cggaacggcg ggcctgagaa ttacggctga
    10381 taataaccca cgccaaggtc attttctttg cgagtacggg caatcaccga ttcgggtgtt
    10441 tctgccacgc gcagacccat ttcatcttca gtacgcacca ctttaccgcg cagagagttc
    10501 gggtagacgt cggtaatttc tacatcgacg aatttaccga tcatatccgg tgtgccctcg
    10561 aagttgacca cacggttatt ttcggtacgc ccggaaagct ccatgatgct cttacgcgat
    10621 gtaccttcta ccagaatacg ctgggtggtg ccgagcatcc gacggctcca tgccatcgct
    10681 tgctgattga tacgttcttg cagaatatac agacgctgct tcttctcttc ttccggaaca
    10741 tcatcaacca tatcagcggc aggcgttcct ggacgtgcag agaagataaa gctgtagctc
    10801 atgtcgaaat tgacgtcggc aatcagcttc atcgttttct cgaagtcttc ggtggtttcg
    10861 ccagggaagc caacaatgaa gtcagaactg atctgaatat ccggacgcgc cgcacgcagt
    10921 ttacggatga tcgctttgta ctccagtgcc gtatgagtac ggcccatcag gttcagaatg
    10981 cgatcggaac cgctctgtac cggcagatgc aggaagctca ccagctccgg cgtgtcgcga
    11041 tacacttcga tgatatcgtc ggtgaattcg atcggatggc tggtggtaaa gcgaatacga
    11101 tcgatcccgt cgatcgcagc aaccagacgc aacagatcgg caaacgatcc ggtggtgccg
    11161 tcgtagttct caccacgcca ggcattcaca ttctgaccga gcaggttgac ttcacgcacg
    11221 ccctgagccg caagctgggc gatctcaaac agaatatcgt cggacggacg gcttacctct
    11281 tcaccacggg tgtaaggcac cacgcagtag gtgcaatatt tattgcagcc ttccatgatg
    11341 gagacaaacg cggtcggccc ttcggcgcgc ggttccggca gacggtcaaa cttctcgatt
    11401 tccgggaagt tgatatctac aaccgggctg cggtcgccgc gcacggagtt gatcatctcc
    11461 ggcagacggt gcagcgtttg cggcccaaaa ataatatcga catagtgggc gcgctggcga
    11521 atgtgctcgc cttcttgcga tgccacgcag ccaccgacgc cgataatcag gtctggattc
    11581 ttctctttta acagtttcca gcgacccaac tgatggaaga ctttttcctg agccttctcg
    11641 cggattgagc aggtgttcag cagcagcaca tccgcttctt ccgccacgtc ggtcagttga
    11701 tagccgtggg tggcatccag cagatcggcc atcttcgatg aatcgtactc gttcatctga
    11761 cagccccagg ttttaatatg gagttttttg gtcatcgact tgctcttgcg aaatagtagc
    11821 caggaatgca gggcgcatag tgtaatgctt tgctgccgtt gtgaccagta tgagcgttat
    11881 cagcccttgg gggtaaaaat cctgtaaact taaagcatat tgctaacagg atgattgacc
    11941 atgacaaatc aaccaacgga aattgccatt gtcggcggag gaatggtcgg cggcgcactg
    12001 gcgctggggc tggcacagca cggattttcg gtaacggtga tcgagcacgc agaacctgca
    12061 ccgtttgtcg ctgatagcca accggacgtg cggatctcgg cgatcagcgc cgcatcggta
    12121 tcattgctta aagggttagg ggtctgggat gcagtacagg ctatgcgttg ccatccttac
    12181 cgcagactgg aaacgtggga gtgggaaacg gcgcatgtga tgtttgacgc cgctgaactt
    12241 aagctgccgc tgcttggcta tatggtggaa aacaccgtcc tgcaacaggc attgtggcag
    12301 gcgctggaag cgcatccgaa agtaacgtta cgtgtgccag gctcgctgat tgcattacat
    12361 cgagataatg atcttcagga gctggaactg aagggcggtg aagtgattcg cgcgaagctg
    12421 gtgattggtg ctgacggcgc aaattcgcag gtgcggcaga tggcgggaat tggcgttcat
    12481 gcctggcagt atgcgcagtc gtgtatgttg attagcgtac agtgtgagaa cgatcccggc
    12541 gacagcacct ggcagcaatt taccccggac ggaccgcgtg cgtttctgcc gttgtttgat
    12601 aactgggcat cgctggtgtg gtatgactct ccggtacgta ttcgccagtt gcagaatatg
    12661 aatatggcgc aactccaggc ggaaatcgcg aagcatttcc cgtcgcgtct gggttacgtt
    12721 acaccgcttg ccgctggtgc gtttccgctg acgcgccgac atgcgttgca gtatgtgcag
    12781 ccggggcttg cgctggtggg cgatgccgcg cataccatcc atccgctggc ggggcagggg
    12841 gtgaatcttg gttatcgtga tgtcgatgcc ctgattgatg ttctggtgaa tgcccgcagc
    12901 tacggtgaag cgtgggccag ttatcctgtc ctcaagcgtt accagatgcg gcgcatggcg
    12961 gataacttca ttatgcaaag tggtatggat ctgttttatg ccggattcag taataatctg
    13021 ccgccgctgc gttttgtgcg taatctcggg ttaatggcgg cagagcgtgc tggcgtgttg
    13081 aaacgtcagg cgctgaaata tgcgttaggg ttgtagcctt acaacattgc cggatgcgtg
    13141 ccaaccgtag gtcggataag acgcggcagc gtcgcatccg acattgaagg ataagacgtg
    13201 tcaacatcgc attcgacatt gaatgaacgc agaaaagcaa aaagctcgcc gaagcgagct
    13261 tttttaat
//
LOCUS       DAHBHC010000110        13222 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHBHC010000110 DAHBHC010000000
VERSION     DAHBHC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.111"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            110..400
                     /locus_tag="M2A35_004383"
     CDS             110..400
                     /locus_tag="M2A35_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCB7200600.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNNL"
     gene            424..1755
                     /locus_tag="M2A35_004384"
     CDS             424..1755
                     /locus_tag="M2A35_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCB7200601.1"
                     /translation="MITDLILHNNPRMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRNVSITHIPMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            2079..3467
                     /gene="narU"
                     /locus_tag="M2A35_004385"
                     /pseudo
     CDS             2079..3467
                     /gene="narU"
                     /locus_tag="M2A35_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3549..7289
                     /gene="narZ"
                     /locus_tag="M2A35_004386"
     CDS             3549..7289
                     /gene="narZ"
                     /locus_tag="M2A35_004386"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCB7200602.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7286..8830
                     /gene="narH"
                     /locus_tag="M2A35_004387"
     CDS             7286..8830
                     /gene="narH"
                     /locus_tag="M2A35_004387"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCB7200603.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLYISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8830..9525
                     /gene="narW"
                     /locus_tag="M2A35_004388"
     CDS             8830..9525
                     /gene="narW"
                     /locus_tag="M2A35_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCB7200604.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            9522..10202
                     /gene="narI"
                     /locus_tag="M2A35_004389"
     CDS             9522..10202
                     /gene="narI"
                     /locus_tag="M2A35_004389"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCB7200605.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRSGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10281..11174
                     /locus_tag="M2A35_004390"
     CDS             10281..11174
                     /locus_tag="M2A35_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCB7200606.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(11270..12115)
                     /gene="nhoA"
                     /locus_tag="M2A35_004391"
     CDS             complement(11270..12115)
                     /gene="nhoA"
                     /locus_tag="M2A35_004391"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCB7200607.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDGWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            12288..12857
                     /locus_tag="M2A35_004392"
     CDS             12288..12857
                     /locus_tag="M2A35_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCB7200608.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(12861..13088)
                     /gene="pptA"
                     /locus_tag="M2A35_004393"
     CDS             complement(12861..13088)
                     /gene="pptA"
                     /locus_tag="M2A35_004393"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCB7200609.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         3342 a         3313 c         3287 g         3280 t
ORIGIN      
        1 ttgtttttaa tcaccattct attttaaaaa cagtatataa tttataaatg acgccgttta
       61 aaattcgttt agaaaatacg tttaacgata attaaaagga ttctgttaaa tgaaattaaa
      121 aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac gtagcacacg ctgccgaagt
      181 ctataacaag gatggtaata aactcgacct ttatggcaag gttaccgctc tacgttattt
      241 tactgatgat aagcgtgacg atggtgataa aacttatgcc cgtctcggct ttaaaggaga
      301 aacgcaaatc aatgatcaaa tgattggttt tggtcactgg gaatatgatt ttaaaggcta
      361 taacgatgaa gccaacggct cgcgcggcaa caatctctga ttgagttaag tgcccatcac
      421 aatatgatca ctgaccttat attacacaat aatcccagga tgaaaacaat cactttaaac
      481 gacaaccata ttgcacattt aaacgccaaa aacactacag aactggaata tttaaactta
      541 agcaataaca atttactgcc aaccaatgac attgatcaac taatatcatc aaaacatctt
      601 tggcatgtat tagttaacgg catcaacaat gatccacttg cacaaatgca gtactggact
      661 gcagtaagaa atataattga tgacactaat gaagtgacca ttgatttatc aggacttaat
      721 ttaaccactc aaccaccagg gctgcaaaac ttcacctcta tcaatcttga taataaccaa
      781 ctcacacatt ttgatgcaac caactacgat agactcgtaa agctaagtct gaatagtaat
      841 actcttgagt caataaattt tcctcaaggc agaaatgtaa gtattacaca tatacctatg
      901 aataataatg ctctcagaaa tattgatata gataggcttt catcagttac ttattttagt
      961 gcggcacata atcaactaga gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg
     1021 aatctcagcc ataatcaatt aactgatatt gttgcaggta ataaaaatga actcttactg
     1081 ctggatctat cccataataa actaacaagt ttacacaatg acttatttcc caacttgaat
     1141 acgttactta ttaacaacaa tttgctttct gaaattaaaa tattctatag caacttctgc
     1201 aatgttcaga cattaaacgc tgctaacaac cagttgaaat atataaatct tgatttcctg
     1261 acttatcttc catctatcaa aagtttaaga ctggacaata ataaaataac ccacattgat
     1321 actaataata catccgatat tggaacttta ttccccataa taaaacagag caaaaactta
     1381 aattttttaa atgtttctgg gaagaacaat tgccctacta tgcagctcat gttatttaat
     1441 ttgttttccc cagcacttaa gcttaatact ggcctggcaa ttctttcgcc tggtgcattt
     1501 gaagttcact ctgacggaat agatgtggat aacgaattgt ttcactatcc tattaaaaaa
     1561 gcatataccc catataatat acacacttac aagacagagg aagttgtaaa ccagaggaat
     1621 ataaaagtta aaaacatgac cttagatgaa ataaacaata cttactgtaa taacgattat
     1681 tacaatcagg caataagaga ggaaccgata gaccttctgg acagatcgtt ttcctccagt
     1741 tcatggcctt tttagtgaat aaatcatcac taattctata agtgtctggt cttgttgata
     1801 atatagttaa tatgtaaccc tttagattca cgatctgaag ggttgaaaca tgacatacgt
     1861 tcaaagatta acccccgttt gaaggttagc ttgttagtta catttagtaa cacatatctg
     1921 gctacttaat ccccattctg cccccgcggt tacgctttct tatctattct ttcaatcagc
     1981 acttcaccca attcatcctt aattctaata actggctatt gcgccttacc tcctgttttg
     2041 tcttttttca ttaccaatgt gtgcatgtga ggaacaatat ggcactgcaa aatgagaaaa
     2101 ataatcgtta tcttttacgc gactgaaaac cagaaaatcc gaccttctgg gaaaataaag
     2161 gaaaacatat tgctcgaaga aacctctgga tatcagtcag ttgtctactt cttgccttct
     2221 gtgtctggat gctatttagc gcagttaccg ttaatctcaa taaaatcggt tttaatttta
     2281 ctaccgatca actcttttta ttaaccgcat taccctccgt ttctggcgca ttattgcgtg
     2341 ttccctactc ctttatggtg cctatattcg gtggacgccg atggacggtt tttagtactg
     2401 caatcctgat tattccttgc gtctggctcg gaattgccgt gcaaaatccg aatactcctt
     2461 ttgggatatt tatcgttatc gctttgctat gcggttttgc aggtgcaaac tttgcttcga
     2521 gcatgggcaa tatcagtttc ttctttccaa aagccaaaca agggagcgct cttgggatta
     2581 atggcggatt aggaaactta ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt
     2641 ttgtacctgt atttgccttt ctcggcgtca atggcgtacc gcaggccgac ggttcggtga
     2701 tgtcgctggc gaatgccgca tggatttggg taccgctact ggcgattgcc acgatcgccg
     2761 cctggtcagg gatgaatgat atcgccagtt cacgcgcctc aattgccgac cagctccctg
     2821 tcttacaacg cctgcatctc tggctgctga gcctgcttta ccttgccacc ttcggttcgt
     2881 ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac ccagttcccg gatgtgaata
     2941 ttctgcgcct ggcgttcttt ggcccattta tcggtgccat cgcgcggtcg gttggtggtg
     3001 ctatttccga taagttcggc ggcgtgcggg tgacgttgat caactttatt tttatggcga
     3061 ttttcagtgc cctgctgttc cttaccttac cgggcacagg ctccggtaat ttcatcgcct
     3121 tttacgccgt atttatgggg ctgtttctga ctgcgggtct gggaagtggt tctactttcc
     3181 agatgatcgc cgtcatcttt cgccagataa ccatttatcg ggtaaagatg aaaggcggta
     3241 gtgatgagca agctcagaaa gaagccgtca ccgaaacggc ggcggctctg ggctttatct
     3301 cagccattgg cgcagtgggc ggctttttta ttccgcaggc gtttggcatg tcgctcaata
     3361 tgaccggctc tccggtcggc gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc
     3421 tgctgacctg gctggtttat ggtcggcgga agttcagcca aaaataagag atcgatgcct
     3481 gatgtacaac cgtgtcgcat ccgacaaggc atcagatccc aattcattcc tggagcagga
     3541 gtcatgtcat gagtaaactt ttggatcgct ttcgctactt caaacaaaag ggcgaaacct
     3601 ttgccgatgg tcacggacag gtgatgcata gcaaccgcga ctgggaggac agctatcgcc
     3661 agcgttggca gttcgacaaa atcgtgcgtt ccacccacgg tgttaactgt acaggctcct
     3721 gtagctggaa aatctacgtt aaaaatggtc tggtgacctg ggaaatccaa cagaccgact
     3781 acccgcgcac tcgccctgac ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa
     3841 gttactcctg gtatctttac agcgctaacc gcctgaaata cccgctcatt cgtaaacgac
     3901 tgattgaact gtggcgcgaa gccctcaaac aacacagcga tccggtactg gcgtgggcat
     3961 cgattatgaa cgatccgcaa aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt
     4021 ttatccgctc caactggcag gaactaaacc agctgattgc cgccgctaac gtctggacca
     4081 tcaaaaccta cggcccggat cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg
     4141 tttcttacgc cgccggaacg cgttatctgt cgctgcttgg cggcacctgt ttaagtttct
     4201 acgactggta ttgcgacctg ccgcccgcct cgccgatgac ctggggcgag caaaccgacg
     4261 taccggaatc tgccgactgg tataactcca gctacatcat cgcctggggg tctaacgtac
     4321 cgcagacacg tacgccggac gcccacttct ttaccgaagt acgctacaaa ggcactaaaa
     4381 ccatcgccat tacccctgac tactctgaag tggccaaatt gtgcgaccag tggctggcac
     4441 cgaaacaagg cactgatagc gccctggcga tggcaatggg ccatgtgatt ttaaaagagt
     4501 ttcatctcga taatcccagc gactacttta tcaactactg ccgccgctac agcgacatgc
     4561 cgatgctggt aatgctggag cctcgcgacg atggtagcta cgttcccggg cgcatgatcc
     4621 gcgcatctga cctggtggat ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag
     4681 cagttaatac cgcaggtgaa ttggtagtgc cgaacggttc gattggtttc cgctggggag
     4741 aaaaaggcaa atggaatctg gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa
     4801 ccctgctcgg tcaacatgac gctgttgcag gcgtggcctt cccctacttt ggcggcattg
     4861 aaaatccgca ttttcgcagc gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta
     4921 aaaacctgac gttagtcgat ggcaacacct gtccggtggt cagcgtttat gatttggtac
     4981 tggcgaatta cggcctcgat cgcgggctgg aagatgaaaa cagtgcgaaa gattacgctg
     5041 aaatcaaacc gtacacccca gcctggggtg agcaaattac cggcgtgccg cgccagtata
     5101 ttgaaaccat cgcccgtgaa tttgccgata ccgcccataa aacgcatggg cgctcgatga
     5161 ttatcctcgg cgcaggtgtt aaccactggt atcacatgga tatgaactac cgtgggatga
     5221 tcaatatgct gatcttctgc ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg
     5281 tcggccagga aaaactgcgc ccacaaaccg gctggttgcc actggccttt gcgctcgact
     5341 ggaaccgacc accgcgccaa atgaacagca cctcgttttt ctacaatcat tccagccaat
     5401 ggcgctatga aaaagtctct gctcaggagt tactttcacc gctcgccgat gccagtaagt
     5461 acagcggtca tctgattgat ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg
     5521 cgccgcagct ggggcgtaac ccgctcggga ttaaagctga agccgacaag gccggattat
     5581 cccccacaga atttaccgcc caggcgctga aatcgggcga tttacgtatg gcctgcgaac
     5641 aaccagatag cggcagcaat catccgcgta atttgtttgt ctggcgctct aacctgcttg
     5701 gctcctccgg caaaggccac gagtatatgc agaagtatct gctggggacc gaaagcggga
     5761 ttcagggcga ggaactcggt gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa
     5821 ctgcggcgat tgaaggcaag ctcgacctgc tggtgacgct cgacttccgc atgtccagta
     5881 cctgcctgtt ctccgatatc gttctgccca ccgccacctg gtacgaaaaa gacgatatga
     5941 acacctcgga tatgcatccg tttattcatc cgctttctgc ggcggtcgat ccggcgtggg
     6001 aatcacgcag cgactgggaa atctacaaag gtatcgccaa agcattttcg caagtgtgcg
     6061 tgggtcatct tggcaaagaa accgacgtgg tattacaacc cctactgcat gactctccgg
     6121 cagagctctc acagccgtgt gaagtgctcg actggcgcaa aggcgaatgc gatctgattc
     6181 caggcaaaac cgcgccgaat attgtggcgg tggagcgcga ctaccctgct acgtatgaac
     6241 gctttacctc gctcgggcca ttgatggaca aacttggcaa tggcggtaaa gggatttcgt
     6301 ggaatacgca ggatgaaatc gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc
     6361 cggcacaggg gcgtccgctg attgacaccg ccattgacgc ttcagaagtg attctggcac
     6421 tggcaccgga aaccaacggt catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca
     6481 ccgggcgcga acatacccat ctggcgctgc acaaagagga cgagaagatt cgctttcgcg
     6541 atattcaggc gcagccgcgt aaaattatct ccagccccac ctggtccggt ctggaaagcg
     6601 atcacgtatc ctataacgcg ggatacacca acgttcatga gttaattccg tggcgcacgc
     6661 tgtcgggacg ccagcagctc tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc
     6721 tggtggctta tcgcccgcct atcgacaccc gtagcgtcag tgagatgcgc cagataccgc
     6781 caaacggctt cccggaaaaa gcacttaact tcctgacgcc gcaccagaaa tggggcattc
     6841 actcaaccta cagtgaaaac ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct
     6901 ggatcagcga aacagatgcc cgagaactga ccattgtcga taacgactgg gtggaagtat
     6961 tcaacgccaa cggcgcgctg actgcccgcg cggtggtcag ccaacgtgta ccgccgggca
     7021 tgaccatgat gtatcacgcc caggaacgca ttatgaatat tcctggttcg gaagtaactg
     7081 gcatgcgcgg cggcattcat aactcggtta cccgcgtttg cccgaaaccg acgcatatga
     7141 ttggcggtta cgcgcagctg gcctggggct ttaactacta cggcaccgtc ggatcgaacc
     7201 gcgatgagtt catcatgatc cgcaagatga agaacgttaa ctggctggat gatgaaggtc
     7261 gcgatcaggt acaggaggcg aaaaaatgaa aatccgttca caagtcggca tggtgcttaa
     7321 cctcgacaaa tgtatcggct gccatacctg ttcggtgacc tgtaaaaacg tctggaccgg
     7381 gcgcgaaggc atggagtacg catggtttaa caacgtcgaa accaaaccgg gcattggtta
     7441 tccgaaaaac tgggaagatc aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg
     7501 caagatccgt ccgcgtctgg gcagcaagat gggcgtaata accaaaatct tcgccaaccc
     7561 ggtggtgccg cagattgatg attactacga acctttcact ttcgactacg aacatttgca
     7621 tagcgcaccg gaaggcaaac atatccctac tgctcgcccg cgctcgctga ttgacggtaa
     7681 gcggatggac aaagtgatct gggggccaaa ctgggaagaa ctgctgggcg gcgagtttga
     7741 aaaacgtgcc cgcgaccgca acttcgaggc catacaaaag gagatgtacg ggcagtttga
     7801 aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac tgcctcaatc ccagttgcgt
     7861 ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa gacggcattg tgctgattga
     7921 tcaggataaa tgccgtggct ggcgtttgta cattagcggt tgtccgtaca aaaaaatcta
     7981 cttcaactgg aaaagcggca agtcagaaaa atgtatcttc tgttatccgc gaattgagtc
     8041 aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc atccggtatc tgggcgtgct
     8101 gctttacgac gccgaccgca tcgaggaagc ggcgagcacc gagcgcgaag ttgacctcta
     8161 tgaacgtcag tgcgaagtgt tcctcgatcc acacgatccc tcagtgatcg aggaagccct
     8221 gaaacaaggt attccacaaa acgtgattga agctgcccag cgttcgcctg tctacaaaat
     8281 ggcgatggac tggaaactgg cgctaccgct gcaccctgaa tatcgcaccc tgccgatggt
     8341 ctggtacgtt cctccgctgt cgccgattca gtcctacgca gatgcgggcg gtttgccgaa
     8401 aagcgaaggc gtgctgcccg ccatcgaaag cctgcgtatt ccggtgcaat atctcgccaa
     8461 tatgttgagt gccggcgata ccggtccggt actgcgggcg ctgaaacgga tgatggcgat
     8521 gcgccactat atgcgttcac aaaccgtgga aggcgttact gatactcgtg ccatcgacga
     8581 agtaggcctg agcgtcgccc aggtcgaaga gatgtatcgt tacctcgcca ttgccaacta
     8641 tgaagatcgt tttgtcatcc cgacgagcca tcgggaaatg gcgggcgatg ccttcgcaga
     8701 acgcaacggc tgcggtttta cctttggcga cggttgccac ggttcggaca gtaaattcaa
     8761 cctgttcaac agtagccgta tcgatgccat caacatcacc gaagtgcgcg acaaagcgga
     8821 gggcgaataa tgcagatcct caaagtgatc ggcctgttga tggagtatcc ggacgagctg
     8881 ttgtgggaat gcaaggagga cgcgctggcg ttgatccgca gcgacgcgcc gatgcttacg
     8941 gatttcaccc acaacctgct taacgcgccg ctgctggata aacaggccga atggtgcgaa
     9001 gtgtttgacc gtggacgtac cacatcgctg ctgctgttcg aacatgttca tgccgagtcc
     9061 cgcgatcgcg gccaggcaat ggtggacctg ctggcggagt atgaaaaggt cggcctgcag
     9121 ctggattgtc gggaactgcc cgattatcta ccgctgtatc tggagtattt aagcgtactg
     9181 cctgacgatc aggcgaaaga aggattgctc aatgttgcgc cgatcctcgc cctgcttggc
     9241 ggtcgcttaa aacaacgcga ggcaccgtgg tacgcgttgt ttgatgctct gctgcaactg
     9301 gcgggaagct ccctttcaag tgacagtgtc acaaaacaag taaacagcga agagcgcgat
     9361 gacacccgcc aggcgcttga tgcagtgtgg gaagaggaac aggtgaagtt tattgaagat
     9421 aacgccacgg cgtgtgacag ctcgccgctt aatcaatatc agcgacgctt tagccaggat
     9481 gtcgcgccgc aatatgtcga catcagtgcg ggaggtggga aatgattcag tatctgaacg
     9541 tcttttttta cgatatctac ccgtacattt gtgcgacggt gtttttcctc ggcagctggc
     9601 tgcgctacga ctacgggcag tacacctggc gcgcctcctc aagtcaaatg ctcgataaac
     9661 gcgggatggt tatatggtcg aatctgttcc atatcggcat tttggggatc ttcttcgggc
     9721 acctgttcgg catgttaacg ccgcactgga tgtacgcgtg gtttttgcca gtggcagcga
     9781 aacaactaat ggcgatggtg ctcggcggta tttgcggtgt gttgacattg attggcggcg
     9841 caggactgct gtggcgcagg ctgacaaatc agcgcgtgcg tgcaacttcc actacgccgg
     9901 atattatcat catgagcatt ttgctgatcc agtgtctgct ggggctaagt accataccgt
     9961 tttcagcaca gtatcctgat ggtagcgaaa tgatgaagct ggtgggttgg gcgcaaagca
    10021 ttgtcacttt ccgtagtgga tcgtcagaaa tgcttaacgg cgtagcgttc gttttccgcc
    10081 tgcatctggt attgggaatg accatcttcc tgctcttccc gttcaccaga ttggtgcatg
    10141 tgtggagcgc gccgtttgag tactttactc gtcggtatca gattgtgcgt tcgcggcgat
    10201 aatttcaccc tggccggata gcccttcgct ctccggccac ttgcaggcta tgatagtttt
    10261 ccagtcacag gagaacgtta atgaaaccgc aggtgtacca cgtcgatgct tttacctctc
    10321 aaccatttcg cggcaattct gccggtgttg ttttccccgc cgataatctt agcgaagcgc
    10381 aaatgcagct tatcgcccgc gagttaggcc attcggaaac tgcttttctg ctgcacagcg
    10441 acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagtgccg atttgcggtc
    10501 acgcgacagt agctgcgcac tatgtacgtg cgaaggtgtt aggtttagga aattgcacga
    10561 tctggcaaac atcgctggca ggaaaacatc gcgtgactat cgaaaagcac aacgatgatt
    10621 atcgtatttc gctggaacaa ggtacgccgg gctttgagcc accgctggaa ggtgaaacac
    10681 gtgaggcgat tatcaacgcg cttcatctca ctgaagacga tattttgccg ggcttgccaa
    10741 ttcaggtcgc aacaacagga cactcaaaag tgatgatccc gttgaaaccg gaagtggata
    10801 tcgacgccct ttcgcctgat cttgctgcgc tgagcgctat cagcaaacag attggctgca
    10861 atggtttctt cccgttccag atccgcccag gcaaaaacga aaccgatggt cgtatgttct
    10921 cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc ccgatgggtg
    10981 catggttggt acatcacaac gtattgcccc acgatggcaa cgtgttatgt gttaaaggcc
    11041 atcaggggcg cgcattgggg cgtgacggca tgattgaagt gacggtgaca attcgtgata
    11101 accaaccgga aaaagtcacc atttcaggca cagcggtgat tttgtttcat gccgaatggg
    11161 caattgaact ctgaaaccgt tgcctgatgc gcttcgcctg tcaggtctac aaccgagcta
    11221 atgtatgccg gataaggcgt ttacgccgca tccggcaacc tgacataaat tattttcccg
    11281 cctccggatg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca gtaaagccat
    11341 gtttcgcatc atccacgccc agaccaaact gttcctgcat cacggcatat aacgacacca
    11401 catccggtag atttcgctgc tccaccgcgt gcccattttc ataatgggta aaatgaaaat
    11461 tggtcagcgt tagcttgcca ccgtccggca aatggcggca catcagcaaa tgatggcgaa
    11521 aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg ctttgttgct
    11581 gctcgcagag atcaaaacgg tacatcgact gccaatgctg atgatgatta aactgcaaca
    11641 cccaaccatc accctcctgc aacaaccgat actctccgtg tggcgtggtc tgcacgagat
    11701 cggaaactaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca tcagcaatcc
    11761 atttttcctc ttccagttcc accaacagca aacgatgggt gcgcggcggt aatgctggcg
    11821 gatttgataa cactacgcgc cctaacaagc tgcgaacgtt aaaccccagc tcgcgtaaca
    11881 cccgctcaaa cacgccattc tgttcaaaac agtaaccgcc acgacgggct atcaccagtt
    11941 tctcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg tcaaggtttt
    12001 caaacggaat agtgcaattg tgtttcaggt gcaatgcacg aagcgtatca atattgaccg
    12061 cagcagctcc cgaccagtta atacgggcaa aatagtgatt cagaatgggc gtcatcaggc
    12121 gtttccctta gtggttttct caacttataa cctggaatag acgccgcacc ggctattttg
    12181 tgcgatcgcg gacatgcatc acgtttgcaa atcgccaatc ccgttaattt cgttacttat
    12241 tgcgttacgg actacgctta aaaaaacctt cttcgcgagg cgataccatg agccgattca
    12301 tccccataga attacaccat gccagccgtc tgttaaatca tggcccaact gtcatgatca
    12361 ccagtttcga tgagcaatcc cagcggcgta acattatggc agcagcctgg tcaatgccgg
    12421 tggagtttga accgccacgc gtggcgattg tggtagataa atcgacatgg accagggagt
    12481 tgattgaacg taacggtaaa tttggcatcg ttatcccggg cgttgcagca actaactgga
    12541 cgtgggcagt gggaagcgtg tcgggtcgtg aagaagataa atttaattgc tatggcattc
    12601 cggtggtgaa aggtccggtg cttggtttgc cacttgttga agagaaatgc ctggcgtgga
    12661 tggagtgccg cctgctacct gcgacttctg cgcaacaaaa atacgacacg ctgtttggcg
    12721 aggtggtatc agcagcggca gacgcacggg tatttgtcga aggccgctgg cagtttgatg
    12781 atgataagct caatacgttg catcatttag gtgctggcac gtttgttact agcggcaagc
    12841 gtgttacggc aggttaagca ttacatgctg taaccgggtt tctttatcaa agcctccatt
    12901 tggggcgcga tttcggcatc ccagatagct tgccaagatt ctggttgaat ctgctgtaga
    12961 gcaatgctta tcgaactgtc tttactgttc agatggcgaa taataacgtc ggtaatatct
    13021 gcagcaagtg ctgctttttg ttgttcgtcc agttcacgcg gaaagcattt aatgtcgatg
    13081 tgcggcatga ttacgctcct tttgaaagat gtcagtaaaa ttaacacaca ttgtgaatgt
    13141 catgtgtccg gtaaagcgtt tatacgacaa taatgccagg gcaagattac gaaagccttc
    13201 tccccgcaag gactgacgcc ag
//
LOCUS       DAHBHC010000111        13185 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHBHC010000111 DAHBHC010000000
VERSION     DAHBHC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.112"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="M2A35_004394"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="M2A35_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCB7200610.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="M2A35_004395"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="M2A35_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCB7200611.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="M2A35_004396"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="M2A35_004396"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCB7200612.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="M2A35_004397"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="M2A35_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCB7200613.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTHPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3241..4635
                     /gene="ychO"
                     /locus_tag="M2A35_004398"
     CDS             3241..4635
                     /gene="ychO"
                     /locus_tag="M2A35_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCB7200614.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4636..5286)
                     /gene="narL"
                     /locus_tag="M2A35_004399"
     CDS             complement(4636..5286)
                     /gene="narL"
                     /locus_tag="M2A35_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCB7200615.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5279..7075)
                     /gene="narX"
                     /locus_tag="M2A35_004400"
     CDS             complement(5279..7075)
                     /gene="narX"
                     /locus_tag="M2A35_004400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCB7200616.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7414..8805
                     /gene="narK"
                     /locus_tag="M2A35_004401"
     CDS             7414..8805
                     /gene="narK"
                     /locus_tag="M2A35_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCB7200617.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9321..13064
                     /locus_tag="M2A35_004402"
     CDS             9321..13064
                     /locus_tag="M2A35_004402"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCB7200618.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3221 a         3289 c         3457 g         3218 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcatcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc tcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat aagaggatgt gtgctaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catcaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacattca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt ttttcattat taccggaagt taccgacgtt
     3241 ttgagccgtt tcgttcctcg cattatcccg ttttatttac tcttgcttgt agcaggcggt
     3301 acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga taataacaat
     3361 gatggtctgc cggatttagg catggctccc gaaaatcatg atggggaaaa acactttgct
     3421 gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggctgga tactggcgag
     3481 caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc ttagtcaaca ggttaatcag
     3541 cacgtagagt cctggctatc accgtgggga aatgccagtg ttgacgtcaa agtggataac
     3601 gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt
     3661 tatctcacct ggagccagct tggtcttact cagcaggatg atgggttggt gagcaatgtg
     3721 ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg gttataacac tttttatgac
     3781 aacttgctgg acgaaaatct tcagcgagcg ggctttggtg ccgaagcgtg gggcgaatat
     3841 ttgcgactat cggcaaactt ttatcagccg tttgctgcat ggcatgaaca gacagccacg
     3901 caggaacaac ggatggcgcg cgggtatgac ctgacagccc ggatgcgcat gccgttctat
     3961 caacacctca ataccagtgt cagcgtagaa cagtattttg gtgatcgtgt tgatttgttt
     4021 aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa ttacacccct
     4081 gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga gaatcaaaat
     4141 aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg
     4201 ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat
     4261 aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg
     4321 ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac
     4381 ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac gccgggcgca
     4441 caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa cggggaaggg
     4501 gcaagcaatc actggcgatt atcggtggtg gtggaagata accaggggca gcgtgtctcc
     4561 tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg
     4621 cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg cttccacgcg
     4681 agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg tgctttcggt
     4741 gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat
     4801 atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac ggttagcgcg
     4861 caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc cagcagctgc
     4921 ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat agccatccgc
     4981 gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga ataccacaat
     5041 gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt tcatgccggg
     5101 catattgaga tctaacagga tcagatcggg atcaagagac tccgccagtt caataccctg
     5161 ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga taagctgttt
     5221 tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg atagtagccg gttcctgatt
     5281 actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag gtgaccacca
     5341 cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac
     5401 ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca ggcacgccgc
     5461 agccattatc ctgaacggtc agttttacct gattatcgtt ttgcgccacc gtcaccacga
     5521 cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca atttgcaaca
     5581 agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa ttgataatcc agcttcaccg
     5641 ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga cgtaatccag
     5701 gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc caggatgcat
     5761 tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc gcatcgccct
     5821 gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca atagaatcat
     5881 gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc tgacgttcct
     5941 gatggcgatc cagcgccagc gtggcggtga gttgttcaac cagggtatcc accagttgtt
     6001 gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg
     6061 tatgagagtc agccagccgc cacttcaggg tcgtgccgcg atcgccaacg ggtaatacgc
     6121 cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggctgg caggtaaact
     6181 cctgatgatt ctcttcatcg tcagtatcat aaacccgcag ttcgatatca cgtagcaggg
     6241 ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg gcgcgggaat
     6301 gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc tccagcccgg
     6361 cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt tctgcagaca
     6421 tattgttcaa tgcagttcca agcatcgcca tttcgttgcg cccgctgatg ttcgcgcgtt
     6481 gggtaaaatc gcgatggctg acggcactcg ccattgccag cagttgccgc cacggttgta
     6541 gcagtcgcgc ccgcaaccaa ataatagtga acaccagtaa aagtgccata aataccgcca
     6601 ttacccgatg gaccagtacc actgtctcaa tgcgcatttc cgtggtgcgg tcaaaaccag
     6661 ataccagctg atcaagcccg gcaacaaact ggctgacatc cgctgacacc gtttcgcggt
     6721 tttgtgcacg tatcagcgca gggatcagtt cattacgcca gtaatcttgt aaaccctgta
     6781 attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa ctcggcgcta aatgccgttt
     6841 gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct gccaacagac
     6901 ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc
     6961 cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta gaaagcaaca
     7021 caataagcgc aacctgatta accagggtga gcggagagag acaacgtttg agcatgtaaa
     7081 cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat acattctgga
     7141 atggcagtat tctcggctat tggctgaagt atacccatac ccggaaggag ttactcctta
     7201 tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattttacag caccgtgaaa
     7261 aatctcataa tttttatgaa atcactgtac tcactatggg taatgataaa tatcaatgat
     7321 agataaagtt atcttatcgt ttgatttaca tcaaattgcc tttagctaca gacactaagg
     7381 tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatccgc ccccgaaagg
     7441 gctactggag ctgtcattac agattggcga ccggaagatc ctgcgttctg gcaacaacgc
     7501 ggtcaacgta ttgccagccg caacctgtgg atttccgttc cctgtctgct gctggcgttt
     7561 tgtgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg ttttaatttt
     7621 acgaccgatc agctatttat gttgactgcg ctgccttcag tttctggcgc gttattacgt
     7681 gttccatact cctttatggt tcctatcttc ggtggtcgtc gctggacggc gttcagcacc
     7741 ggtattctga ttattccttg tgtctggctg ggttttgccg tgcaggatac ctccacgcct
     7801 tatagcgtct tcatcatcat ctctctgctg tgcggctttg ctggcgcgaa cttcgcctcc
     7861 agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc gctgggtctg
     7921 aatggtggtc tgggcaacat gggcgtcagc gtcatgcagt tggttgctcc gctggtggta
     7981 tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga tgggactgag
     8041 ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt caccattgcg
     8101 gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga gcagttgccg
     8161 gtactcaaac gggggcatct gtggattatg agcctgctgt atctggcaac cttcggctcc
     8221 tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa cgcagttccc ggatgttcag
     8281 attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt
     8341 gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat tctgatggcg
     8401 attttcagcg gcctgctgtt cctgacctta ccgactgacg ggcagggcgg aagctttatg
     8461 gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg ggctgggtag tggttccact
     8521 ttccagatga tttcagtgat cttccgtaaa ctgacaatgg atcgcgtgaa agcagaaggg
     8581 ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc gttgggtttc
     8641 atctctgcga ttggcgcgat tggtggcttc tttatcccga aagcgtttgg tagctcgctg
     8701 gcattaacgg gttcgccagt cggcgcaatg aaagtatttt tgattttcta tatcgcctgc
     8761 gtagtgatta cctgggcggt atatggtcgg cattctaaaa aataaaccgt tactcgtcat
     8821 acttcgggtt acatgtgctg cggctgcgtt cattcacccc agtcacttac tttagtaagc
     8881 tcctgggatt cattcacttg ccgccttcct gtaaaccgaa ttatatagag taaaatattt
     8941 gattatcctt tgcgcggcat gatgtcgcgc tttttttatg cgtcatttag ttacaacata
     9001 ctaatgttgt atggtttatt tcgccggatt tcattaagag ccattaatat gttacccatg
     9061 gggaatactc cttaataccc atctgcataa aattcttaat agtttaaata actacaggtg
     9121 taaaacgtct taatttacag catgttatgc tttaactgtt aattatccta aaggggtatc
     9181 ttaggaattt actttatttt tcatccccat cacacttgat cgttatcaat tcccacgctg
     9241 tttcagagcg ttaccttgcc cttaaacatt agcaatgtcg atttatcaga gggccgacag
     9301 gctcccacag gagaaaaccg atgagtaaat tcctggaccg gtttcgctac ttcaaacaga
     9361 agggtgaaac ctttgccgat gggcatggcc agcttctcaa taccaaccgt gactgggagg
     9421 atggatatcg ccagcgttgg cagcatgaca aaatcgtccg ctctacccac ggggtaaact
     9481 gcaccggctc ctgcagctgg aaaatctacg tcaaaaacgg tctggtcacc tgggaaaccc
     9541 agcagactga ctatccacgt actcgcccgg acctgccaaa ccatgaacct cgcggctgcc
     9601 cgcgcggtgc cagctactcc tggtatcttt acagcgccaa ccgcctgaaa tacccgatga
     9661 tgcgcaaacg cttgatgaaa atgtggcgtg aagcgaaggc gctgcatagc gatccggttg
     9721 aggcatgggc ttctatcatt gaagacgccg ataaagcgaa aagctttaag caggcgcgtg
     9781 gacgcggtgg ttttgttcgt tcttcctggc aggaagtgaa cgaactgatc gccgcatcta
     9841 acgtttacac catcaaaaac tacggcccgg accgtgtcgc tggcttctcg ccaattccgg
     9901 caatgtcgat ggtttcttac gcatcgggtg cacgctatct ctcgctgatt ggcggtactt
     9961 gcttaagctt ctacgactgg tactgcgacc tgcctcctgc gtctccgcaa acctggggcg
    10021 agcaaacaga cgtaccggaa tctgctgact ggtacaactc cagctacatc atcgcctggg
    10081 ggtcaaacgt gccgcagacg cgtaccccgg atgctcactt ctttactgaa gtgcgttaca
    10141 aagggaccaa aactgttgcc gtcacgccgg actacgctga aatcgccaaa ctgtgcgatc
    10201 tgtggctggc accgaaacag ggcaccgatg cggcaatggc gctggcaatg ggccacgtaa
    10261 tgctgcgtga attccacctc gacaacccaa gccagtattt caccgactat gtgcgtcgct
    10321 acaccgacat gccgatgctg gtgatgctgg aagagcgcga cggttactac gccgcgggtc
    10381 gtatgctgcg cgctgctgat ctggtggatg cgctgggtca ggaaaacaat ccggaatgga
    10441 aaactgtcgc ctttaatacc aatggtgaaa tggttgcgcc gaacggttct attggcttcc
    10501 gctggggcga gaagggcaaa tggaatcttg agcagcgcga cggcaaaact ggcgaagaaa
    10561 ccgagctgca actgagcctg ctgggtagcc aggatgagat cgctgaggtg ggcttcccgt
    10621 actttggtgg cgacggtact gaacacttca acaaagtgga actggaaaac gtgctgctgc
    10681 acaaactgcc ggtgaaacgc ctgcaactgg ctgatggcag caccgccctg gtgaccaccg
    10741 tttatgatct gacgctggca aactacggtc tggaacgtgg cctgaacgac gttaactgtg
    10801 caaccagcta tgacgatgtg aaagcttata ccccggcctg ggccgagcag attaccggcg
    10861 tttctcgcag ccagattatt cgcatcgccc gtgaatttgc cgataacgct gataaaacgc
    10921 acggtcgttc gatgattatc gtcggtgcgg ggctgaacca ctggtatcac ctcgatatga
    10981 actatcgtgg tctgatcaac atgctgattt tctgcggctg tgtcggtcag agcgggggcg
    11041 gctgggcgca ctatgtaggt caggaaaaac tgcgtccgca aaccggctgg cagccgctgg
    11101 cgtttgccct tgactggcag cgtccggcgc gtcacatgaa cagcacttct tatttctata
    11161 accactccag ccagtggcgt tatgaaaccg tgactgcgga agaactgctg tcgccgatgg
    11221 cggataaatc ccgttatacc ggacacctaa tcgactttaa cgtccgtgca gaacgcatgg
    11281 gctggctgcc gtctgcaccg cagttaggca ctaacccgct gactatcgct ggcgaagcgg
    11341 aaaaagccgg gatgaatccg gtagattaca ccgtgaaatc cctgaaagag ggttccatcc
    11401 gttttgcggc agaacaacca gaaaacggta aaaaccaccc gcgcaacctg ttcatctggc
    11461 gttctaacct gctcggttct tccggtaaag gtcatgagtt tatgctcaag tacctgctgg
    11521 ggacggagca cggtatccag ggtaaagatc tggggcagca gggcggcgtg aagccggaag
    11581 aagtggactg gcaggacaat ggtctggaag gcaagctgga tctggtggtt acgctggact
    11641 tccgtctgtc gagcacctgt ctctattccg acatcatttt gccgacggcg acctggtacg
    11701 aaaaagacga catgaatact tcggatatgc atccgtttat tcacccgctg tctgcggcgg
    11761 tcgatccggc ctgggaagcg aaaagcgact gggaaatcta caaagccatc gcgaagaaat
    11821 tctccgaagt gtgcgtcggc catctgggta aagaaaccga catcgtcacg ctgcctatcc
    11881 agcacgactc tgccgctgaa ctggcgcagc cgctggatgt gaaagactgg aaaaaaggcg
    11941 aatgcgacct gatcccaggt aaaaccgcgc cacacattat ggtcgtagag cgcgattatc
    12001 cggcaactta cgaacgcttt acctctatcg gcccgctgat ggagaaaatc ggtaacggtg
    12061 gtaagggtat tgcctggaac acccagagcg agatggatct gctgcgtaag ctcaactaca
    12121 ccaaagcgga aggtccggca aaaggccagc cgatgctgaa caccgcaatt gatgcggcag
    12181 agatgatcct gacgctggca ccggaaacca acggtcaggt agccgtgaaa gcctgggctg
    12241 ccctgagcga gtttaccggt cgtgaccata cgcatctggc gctgaataaa gaagacgaga
    12301 agattcgctt ccgcgatatt caggcacagc cgcgcaaaat catctccagc ccgacctggt
    12361 ctggtctgga agatgaacac gtttcttaca acgccggtta caccaacgtt cacgagctga
    12421 tcccgtggcg tacgctctct ggtcgtcagc aactgtatca ggatcaccag tggatgcgtg
    12481 atttcggtga aagcctgctg gtttatcgtc cgccgatcga cacccgttcg gtgaaagaag
    12541 tgatgggcca gaaatccaat ggcaacccgg aaaaagcgct caacttcctg acgccgcacc
    12601 agaagtgggg tatccactcc acctacagcg acaacctgct gatgctgact ttaggtcgcg
    12661 gtggtccggt ggtctggttg agtgaagccg atgccaaaga tctgggtatc gccgataacg
    12721 actggattga agtattcaac agcaacggtg ctctgactgc ccgtgcggtt gtcagccagc
    12781 gtgttccggc agggatgacc atgatgtacc acgcgcagga acgtatcgtt aacctgcctg
    12841 gttcggaaat tacccaacag cgtggtggta tccataactc ggtcacccgt atcacgccga
    12901 aaccgacgca tatgatcggc ggctatgccc atctggcata cggctttaac tactatggca
    12961 ccgtaggttc taaccgcgat gagtttgttg tagtgcgtaa gatgaagaac attgactggt
    13021 tagatggcga aggcaatgac caggtacagg agagcgtaaa atgaaaattc gttcacaagt
    13081 cggcatggtg ctgaatctcg ataagtgcat cggctgccac acctgttcag ttacctgtaa
    13141 aaacgtctgg accagccgtg aaggcgtgga atacgcgtgg ttcaa
//
LOCUS       DAHBHC010000112        13018 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHBHC010000112 DAHBHC010000000
VERSION     DAHBHC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.113"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..302
                     /locus_tag="M2A35_004403"
     CDS             <1..302
                     /locus_tag="M2A35_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200619.1"
                     /translation="DWMPVQPDAISGIWTAELDREQRTALLSLNQPFTQEALMYELDA
                     FLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWATVVSPFVSPQEAS"
     gene            359..3685
                     /locus_tag="M2A35_004404"
     CDS             359..3685
                     /locus_tag="M2A35_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HCB7200620.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRY
                     ENIPEGVRQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            3678..5288
                     /gene="tssA"
                     /locus_tag="M2A35_004405"
     CDS             3678..5288
                     /gene="tssA"
                     /locus_tag="M2A35_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCB7200621.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFAAWPEEQQSPSLMPLINALESRLAQSGGMNAVVPQNSSSIPAASSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLELADLMFSTGVSHFWLDIQWYLHQALTKAGVP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            5294..6712
                     /locus_tag="M2A35_004406"
     CDS             5294..6712
                     /locus_tag="M2A35_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCB7200622.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIGGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGSEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            6705..7128
                     /locus_tag="M2A35_004407"
                     /pseudo
     CDS             6705..7128
                     /locus_tag="M2A35_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7688..9448
                     /gene="tssF"
                     /locus_tag="M2A35_004408"
     CDS             7688..9448
                     /gene="tssF"
                     /locus_tag="M2A35_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCB7200623.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGSLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPFYLNADSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENRPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            9412..10491
                     /gene="tssG"
                     /locus_tag="M2A35_004409"
     CDS             9412..10491
                     /gene="tssG"
                     /locus_tag="M2A35_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCB7200624.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQFLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPNVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            10472..11008
                     /gene="tssJ"
                     /locus_tag="M2A35_004410"
     CDS             10472..11008
                     /gene="tssJ"
                     /locus_tag="M2A35_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCB7200625.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            11012..11440
                     /gene="tssE"
                     /locus_tag="M2A35_004411"
     CDS             11012..11440
                     /gene="tssE"
                     /locus_tag="M2A35_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCB7200626.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMVAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     YLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            11440..12816
                     /locus_tag="M2A35_004412"
     CDS             11440..12816
                     /locus_tag="M2A35_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCB7200627.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     RVEKLSLALFRQNGVELQTQVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMKQPEKSEESPQPVFATRPAQQNDK
                     DASPSAPSPEISWQRTWPIFMAGMVVMACLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
BASE COUNT         3075 a         3389 c         3696 g         2858 t
ORIGIN      
        1 tggactggat gccggtgcag ccagatgcca tatccggcat atggactgcc gaactggaca
       61 gggaacagcg taccgcgttg ttatccctta accagccgtt tactcaggaa gccctgatgt
      121 acgagctgga tgcctttctg gggcgctccg gaccggcagc gccgtggctg tccgtcgctg
      181 cggcaacgct ggcggcaata caatcacaac acccccaact aacgctcagc ggcgttcagg
      241 gcgggcatta ttcatgggcg accgtggtgt cgccattcgt ttccccacag gaggcatcat
      301 agtggttccg ggattaaacc gtctccggcc ccgttctgtt tcagggcctg ccatcgtcat
      361 gaccgtggct gttgtggttg cgctttgcac gtttattcac cgtaaaccgg cattgctggg
      421 cgttgaaccg ggtagctcac agcaaatgta ctggatggct ggcgtgattg ccgcaggcgt
      481 ggtctttttg attatcatgg gggtttatca tctgctgaac cgccacgctg ccgtacaaaa
      541 ctttgaactc cgcagacaac ttgccgccgg agatgactat cgtgcggtga cgacaaagaa
      601 agccgacagc cgtcatccgg caatggtgga actcgccgcc ttcctgcgcg accgctacgg
      661 tccgttctgg cggcgtaacg tccgcctgct gctggtgacc ggcgagcctg aacaggcaga
      721 agccatcgcg ccggggctga ccgggcaaca ctggctggag ggcgaccaca cggtgctgat
      781 atatggcggc aggccatcag cggagcctga tgtcacactg ctgaccgcct taaaaaaact
      841 gcgccgcagc cgtccgctgg acggcatcat ctgggcgctg acagaagaac agagccgcca
      901 gacagcgcaa ctcgacaaag gctggcgcga actgataaac ggcggtaagc gactcggttt
      961 tcaggcccca ctctatttgt ggcaggtctg tgacaacggt gattatcagg ccggacgccc
     1021 cctgcaaagc gtcggctgcc tgctgccgga acgctgcacc ccggaacaac tgaccgccat
     1081 gctggaagct cacaccctgc cgctgacgga acagggcatg tcgcaactgc tggctgataa
     1141 ccgccatgat tttctgctgc gtctggccca tacccttgca gagcggggta tcgctcactg
     1201 gcaaagcgtc ctgaaaccgc tgctggcagg cggcgcattc tcttccctgc gcctgcgtgg
     1261 tctgatgttc agcccgccgc ttaccgccgt accggaggcc gcacctcatg cgtggctgcc
     1321 gtcacaggtc tgggcgggcg tgaccggcga taacgcacgg gggcgtgcag tgggctttcc
     1381 gtggctacgc gccgcgctga tgtccgctgt ctgcgtgctg gcaatatggg gggccggaat
     1441 gacgacctct ttcttcgcca actgcgctct tgttcaggaa accggtatcc agacggcgcg
     1501 ggcgctcgat acccgcctgc cgctggcaga acaactggtg gcgctgcata ccctgcaggg
     1561 cgaactggaa cgcctgcaat atcgtatccg cgaaggtgcg ccgtggtatc agcgttttgg
     1621 ccttgaacgt aaccaacaac tgctcgccgc cgcctttccc ggctatgcgc aggcggcaaa
     1681 ccggctggtg cgcgacgtgg ccgttgacca tctgcaacag caactgaacg cctttgtcgc
     1741 cctgccgccc aacagtcctc agcgtaccgc caccggtgaa caacgctata agcagcttaa
     1801 ggcattgctg atgacttccc gcccggaaaa ggccgacgct gcctttttca gtaccacgct
     1861 gatggcagac agcctgcgct acgagaatat cccggaaggt gtgcggcaga gcgtgtttcc
     1921 gtcactgctg accttctgga tggcgaacct gccggaacac ccgcagtgga aaacctcgcc
     1981 gccaccggaa ctgaccggcg cggtgcgtaa aatcctgctg cgccagattg gtgtgcgtaa
     2041 tgccgaaaac actctctacc agaacgtact gcaacaggtg tcccgcaact acgccgatat
     2101 gacgctggcg gacatgaccg gggataccct caccgaatct cttttcagta cggaacagac
     2161 ggtgccgggg atgttcaccc gtcaggcgtg ggaaggacag gtcagggaag ccatcgagca
     2221 ggtggtgacg gcgcggcgcg aggaaatcga ctgggtgctc agcgaccgcc agcaggatac
     2281 ctctgcggat atatcgccgg atacgctgcg taaccgtctc acctcacgct actttaccga
     2341 cttcaccgga agctggctgg cgtttctcaa cagcattcgc tggaaaaagg aagactcgct
     2401 ctccggcatt ctcgaccagc tgacactgat ggccgatgcc cgtcagtcgc cgctgattgc
     2461 gctgaccgat accctcgcct ggcaggcggc ggcaggcagg gaaaaccgtg gcctgtcaga
     2521 ctcgctggcg aaatcggcac aggaactgtt taacggcaag gagaaaacgc cgcagcaatc
     2581 ccgtgaaggc gacgtgcctg tcgggccgct ggataaaacc ttcgctccgc tgctgcggtt
     2641 gctgggcgat aaagccggag gcggcgacag ccagctgagt ctgcagacct acctcacccg
     2701 cgttacccgt gtgcgtctca aactgcaaca ggtgaccaac gcccccgacc cgcaggaaat
     2761 gacgcaacag ctggcacaga cggtactgca gggcaaaacc gttgacctca ccgacacccg
     2821 cgactacgga cggttaatcg ccgccagtct gggcgaggaa tggagtggct tcggtcaggc
     2881 gctgttcgtc cgcccggtgg agcagtcgtg gcggcaggtg ctgacgcctg cggcggacag
     2941 cctgaaccgc cagtggcagc gggcgattgt cagccactgg aatcaggact tcgctggtcg
     3001 ctacccgttc aaagcctcac agaacgatgc ctccctcccc ctgctggcgc agtatctgcg
     3061 cgatgacggg cgcatcaacc agtttattgc cgccaacctt tccggcgttc tgaaacgtga
     3121 aggccgctac tgggtggcgg acgccatgaa cacgcagggg ctgacggtca acccggactt
     3181 tatccgcgcc ctgaaccgcc tgcgcgacgt ggctgacacc gcctttgcca gcggcgatgc
     3241 cggaatacat tttgaactgc aggcaaaacc ggcccgagac gtgatgaaga cacatctggt
     3301 gattgacggg caggaactgg agtatttcaa ccagaaagaa cgctggcagc gtttcaactg
     3361 gccggatgaa cagtggcaac ccggcgcatc gctgagctgg accagtacgc aggcgatgga
     3421 acgcatactg gcggattacc gggggagctg gagccttatt cgcctgctgg aacaggcgca
     3481 ggtgacaccg gtggacagca gcacctttaa agtggtgtgg aaagcgcagg acggcctgcc
     3541 gctgaattac ctgctgcggg ttgaacaggg taaagggccg ctggcgctgc tggaactgaa
     3601 aaacttccgc ctgccgggac aggtatttct gaccggcagg agtatgaaag atgcggaaga
     3661 atacggggaa gacgccgatg agtgaactgt tgcaaaagct gacccgtagc tgttttgtcg
     3721 accgggatgc actggatgtg gcgcgaacac aggcagcgct gtggcagaca tggctgctgc
     3781 cggtgacggc aaatacgccg gtcggggaag accccggcta ccacgacgat ttcctgcgta
     3841 tccgcgacga gatgaacaaa ctttccggcg cggatacgga cctcatctgc cagcttgccg
     3901 aatccctgct gctgacgcag gcaaaagacg tgcgcatcgc cacttactac atctgggcgc
     3961 gactgcaccg cgacggtgag cgcggactgg ctgaggggct ggcgctactg accggactgg
     4021 tggaacgctt cggtacgcaa ctgctgccct cgcgaccggc cagccggaaa atggcgctgg
     4081 agtggctggc aggggaaaag atgctcgaca gccttgcccg ctacccggaa gtggcaaaag
     4141 aggattttgc gaacattgtt gccgctctta atcaactgac ggtttccttc gccgcatggc
     4201 ccgaagaaca acagtctccc tccctgatgc cgctgattaa cgcgctggaa tcgcgtctgg
     4261 cgcagtcggg tgggatgaac gcggtggtgc cgcaaaacag cagcagcata cctgcagcgt
     4321 catccccggt ggatgcgcca caggtacaga ccatcacctc cgggcgtgac ctgctcgacc
     4381 aggcgaaagt gctggcccgt tatctgaacg aacagccgca gggctggctt tcggcacacc
     4441 ggctgatgaa aaccctgcgc tgggacacgg tgcatgaact gccgccggac gtggacggca
     4501 aaacccggct ggccccgccg cgcaccgagt cacgcaacca gcttaagcgg ctgtatgcgc
     4561 agcagaactg gacggaactg ctggagctgg ccgacctgat gttcagcact ggcgtcagcc
     4621 atttctggct ggacattcag tggtatctac atcaggcgct gactaaggcg ggcgtaccgt
     4681 gggacaggtg gacagcggtt atccgtcagg acctggcgct tctgctggag cgcctgccgg
     4741 ggctggaaaa tctggcatgg aacgacggca caccgttcgc cgacgaggtg acgcgcaact
     4801 ggatagcgca gcaggtcatg atgcgggaag acggcgcatg gcttgcgggg aaagccgctg
     4861 tgccgacaga cgacgccaca aatgacgtac tggcgctgga gccggaagca ctggagatgg
     4921 cagacagtca gggcgtggaa gcggctctgg gctggataca gacccgtccg ggcatcacca
     4981 cggcacgcca gcgcctgtta ttgcgactgc tgatggcgcg ggtggcggaa cagtacggta
     5041 aaaacgaaat ggcgctcctg ctgctggaag aactggatac cgccgcacag gggctgacgc
     5101 tgacgcagtg ggagccggac ctgctgtttg aggtaaaagc ccgacaactg aaactgctgc
     5161 gcctgcgggc gcaccgctat gcggataaag ccctgctgaa ccgaaaaatg gaaatcctgc
     5221 tgggaacgct ggtgacaatc gatccggtac gggcagcggt attgtgtgat acgcaacata
     5281 aggactgatg gcaatgggaa caggctactt tctggtcagg ggggacaaaa ccacctgcgg
     5341 cgggaagatt attggaggcg cggacgacca tacgatcatg ggtatcccac aggcacggga
     5401 tatggaccgg gtcacctgcg gcagatatcc gggaatgttt attattgtgg gcggcgttcc
     5461 tgaaacagat attcacggca ggctgatggc ggggacgctg gacagccaga gcagttgccc
     5521 ctgtaaggca cggtttattg cgtcgatgat ggatgatacg tatgagacgg atgacggggg
     5581 aagtgaagcg gaacaacatg cgcagtcggc gaaaaaggat ctgacttctg gttctgattc
     5641 atcctctgat gatgtaaaac tggattacag aattaaatta tcaggtaata agatactgac
     5701 gccactgaat atacctgatt ataaagaaat gataagcggt ggtagtacga agaatacaga
     5761 aaaaatagat tttactatca caaataaagg cgatgaggct gaaggacttt ctctggaagt
     5821 gcttgatggt aatgaagtga tttacagtga acagcagacc ggtaaatatt ttgataaggg
     5881 agagcatgcc tggcaatggg atggctacag taatcaaggc atactggata caactaagct
     5941 aaaaagcaaa tcactactcg taagactgat tgctttatgt ggtgatatga tgataaaagt
     6001 tgattatcca cttcataatt caccagagga acaaaaatgg gtcgatgtaa aggtagacag
     6061 aaaacaaaaa acagtagata taatatggcg actggcagtt agtgatggag ggataaaagg
     6121 aagtaatcct aagttatccc cagttcctta taacgacttg gttaacctga ccaaaaatgg
     6181 agttgaattt tattggtctc gaaatggaag tcgcggcggg gggattggtg aaaatattgt
     6241 tacagcgatt ggtgttttta aagtaaatgt aaaggctgag ataaatatta ctccatccat
     6301 gcgaactttt tctttgatat catcattaga tcctgacttt caggcatccg ttagtctatc
     6361 aggatttgaa aaaatatatt ataactacgg tgatagttat aaggatattc aagatgaatt
     6421 acaggcgctt cttgacgcta ataacagata taaatgggat agtgctcatg aaatggggca
     6481 taaagtactc gatgaatatg gagagggttc tagccctgat tattcgtgga ctcataaagg
     6541 aacatctact cttatgcaaa aaacaatacc agggaatgtt atgcccgctc agggagagat
     6601 tgatgtaatg aaatatggaa aatatcgtcc tgacatgtat actcgattgg ttgcagcaga
     6661 tgaagatgtt cagggattga tctggttaag caggataaaa tttgatgact agagtagtta
     6721 ttgctttatt atttcttgcc ggatgtactt cgcaaatgaa gtattccaat gtcgtcagga
     6781 ttaggaatgt atgttcctca gatattaatg tgtttattcc tgagtatgtt tttggaggta
     6841 atgttattaa gcaacatcat atttacttac ctgttaatgg gattgatgtt ttgtcttatt
     6901 tttatactga aaacatcaat gatggtttcc aatatgaaga agcattcacc gtaaataaag
     6961 atagctcggg taattttgat tatccgtttg ttatcttatc atcaggacgt gaaaaaaaaa
     7021 tttgggaaaa acgatctaat tgaattatcc agggacattt caacaaaaga cgataagaag
     7081 catggggttg taaagtacat gatagatagt caggatattt gcccatagta tgattcatat
     7141 cataatcaac tgaaaaaatg acattattaa tcatttgtga ttttttgata ctaccaagtg
     7201 aaatttgatg attagattaa tattctcttt tttattatgc acactgtatg ctggatgttt
     7261 atctcaacca ttctattctt gttccattgt catggtaaat acatgcaaag tatctattaa
     7321 catttcgaca gtcaataatt caacacatta tgatggtaag gtaagagata attttcatct
     7381 tgatatcgga gagcggaagc caattgtata ctttatttac acaggtagag agtcagatgt
     7441 ttttaataaa aaccctgtaa atattgtttc tgaacttagt attttgatat ctgatggtaa
     7501 caattctaaa actttatctg gatatcatat ctcctcttta ttgaaaaaat cctcatctag
     7561 tatggagtcc gatttttctt ttgaaataaa tgatccatct atatgtcctt aggattctga
     7621 aaataaaaaa ttctaatctc actactcgtt ggtattaatt tcaataaaag gaaagataaa
     7681 tcctccgatg gacgacttaa cccaacgcta ctacgaagcc gaaatgcgct acctgcgtga
     7741 agcggggaaa gaatttgcgc aggcttaccc cgaccgcgcc gcgatgctca atcttgataa
     7801 acccggcgca cgcgatccct atgtggaacg cctgttcgaa ggctttgctt ttttaatggg
     7861 cagcctgcgc gagaagctgg acgacgatct gccggagctg actgaagggc tggtcagcct
     7921 gctgtggcct cactacctgc gaactatccc gtcactttcc gtggttgaac tgtcgacaga
     7981 tcaccggcag atgaagcaga gcgaaacgct gagcgatttt caggtattgt cgcgccctgt
     8041 tggcgagcgc agaacccgct gtttctacag cgccacgcgg gatatcacgc tgcatccgct
     8101 ggcgttgccg gacgtgtcat tgcaatatga accggacgga cgttcggtta ttcgtctgcg
     8161 ctttgaatgc ggcccgctgg tcggtgactg gtcacagatc gatttatccc gcctgccgtt
     8221 ctatctcaat gccgacagtc cggtggcctg tgcgctgcac cgggctttaa cgcttggtac
     8281 gcagcagttc tggttgcgtc tgccggggca ggatcgccgg acgcttgacg cacacttttc
     8341 cccgttgggc tttgaggata atgacaggct ctggccaaaa ggcgagtcgg cgttcagcgg
     8401 ctaccagttg ctgctggagt atttcacctt ccgcgagaag tttatgtttg tggcactcaa
     8461 cgggcttgag ctggttgcat ggccggaggg gataaccggc tttgagattg acgtggtgct
     8521 gaacgagaac aggcctcacg acctgccgtt tgacagcgat aatatccgtc tgcactgcgt
     8581 gccggtcatc aatctgttcc cgctcgaagc cgatccgtta cacctttcgc cactggaaaa
     8641 cgaatttctg ctacgcccga tgcgcattca ggacgggcat accgagattt attccgtcga
     8701 caacattatt tcttcgcgcc ataccggcag tcaggcgtat gtccccttct ccagctttcg
     8761 tcaccggggc gggatgctgc gccacgatgc gccggaacgc tattaccaca cgcgggtaaa
     8821 gcgcggccct tccggtctgc atgacacctg gctgattctg ggcggtgatg cgtttgatgc
     8881 tgaccggatg ctggaggatg aaacgctctc tttaagcctg accggtacta acgggcatct
     8941 tccccgtaag gctttacaga gcacgctgct cgataggcca gttcatgcct cacagaacgt
     9001 cctgcgggtg cgcaacctgt gcgccccaac gcagccctgc tatcccccgg cgcgtgaccg
     9061 cttccactgg cgggtgctca gccaccttgg ttctaacttt ttatcgatga tggataacgc
     9121 cgaaattctg cgcggaacgc tggcgctgta cgactggacc gaaagcgaga tgaaccgccg
     9181 tcgtctggag gcgattgtcg acgttcagca cagcctgata cagcgttttg aaagaggctt
     9241 tctgctgcgt ggcgtggata ttcaggtgac gctggacagt aacggctttg ccggagaagg
     9301 tgatatcacg ctgtttggtg aactgctgca ccgctttttc gcactgtatg ccgatatcca
     9361 cttattcact caactgacct taattctgca acctacagga aagtgcctgc aatggacaga
     9421 gcatcacagc cagcgcgtac cgggctgacc gaccggctgg ccccggatat tgcgcggatc
     9481 aatttttacc gtttttgcca gtttctggaa cagagccagc agaacgcgcc gctgggcagt
     9541 accgacaacc ccgccaccga tgcggtgcgc tttcgtcccc atcccggaat gggctttccg
     9601 gtcagcgagt taaagaacgt tgaacgtgac gcgggcaatc ccaacgtgcc acccacggtg
     9661 cgcaccacct ttctcggcct gtacggcgtg gattcgccgc tgccaaccgc ctatctggac
     9721 tacattaccc agcgtcatga cggacatgac gcagtgatgg cgtttctcga tatttttaac
     9781 catcgcttta tcacccagta ttaccgcatc tggcgcaaat ataactaccc ggcgtcgttt
     9841 gaagcaggtg ctatggatga catttcccgt tgtctgctgg gactcatcgg gctgggtatt
     9901 cccggcagcg agaaccatat cgccacgccg gtatcccgtt ttctggccct gctcagcgtg
     9961 atgcgcctgc cgacgcgcac ggcggaaggc gtcacggcac tggtgggact gcttgccccg
    10021 ctgacgaaag cgacggtggt gcctcacgat ccgcagcctg ttattctccc ggcccctgcg
    10081 ggtctgtcaa aaaattcacg catcagcctg aaaacgcgaa cgctgctggg gcgtaccggc
    10141 accgacgtca acagccagtt gctgctgaaa ctgtataccg aagatgccgc agaagcacgt
    10201 ggctggctac ctggcgggca gctgcacagc gatttgctgg tgctgctgcg cgtttatctg
    10261 ggctggcgct gtcaggcccg tctgcaactg acgctgcccg tcagcctgtt gcctgctgcc
    10321 cggctgggaa aacagcgcgt acagattagc cgtaccggca tccttcgcgc ctcctttgcc
    10381 gcgcccgcga ccggaacagt gacggtcagt ctggggcgtt atcagggatt aatacccgct
    10441 ttatccattc gtaacaggga gagtatgact catgtttcct attcgtttta aacgtccggc
    10501 gctgctctgt atggcgatgc tgacggttgt tctgagcggc tgcgggctga ttcagaaagt
    10561 ggtggatgaa tcgaaaagcg tggcctcagc cgttttctac aagcaaatca aaatactgca
    10621 tctcgatttc ttctcccgaa gtgccctgaa tacggatgcg gaagatacgc cgctttccac
    10681 gatggtgcat gtctggcaac tgaaaacccg cgaagatttt gacaaggcgg attatgacac
    10741 cctgtttatg caggaagaga agacgctgga gaaggacgta ctggcaaaac acaccgtctg
    10801 ggtaaaaccg gaaggcacgg catccctgaa tgtaccgctg gataaagaga cgcagtttgt
    10861 cgccattatt gggcagtttt atcaccctga tgaaaaaagc gacagctggc gtctggtgat
    10921 caaaagggac gaactggagg ccgacaagcc gcgctcgatt gaactgatga gaagcgacct
    10981 gcgactgctg cctctcaagg ataaataacc gatgtcgaca ccttccttat atgaaatgct
    11041 gacgttcagt ttcagcggcg agttaccact ggaacaggtc agcgagcggg atcagcttat
    11101 cctttccgtg atggacaata tgcaacgcat tatcaactgt cgggcaggca cactggcgca
    11161 tttgccggat tacggtctgc cggatctcag tctgatccat cagggtatgg ttgcgggtat
    11221 tcatggcctg atgcgtcaga ttgaagaaac gctgctgcgt tatgaaccac gcctgagtca
    11281 gatacaggtg gagttactcc cccagccccg tccggggtat cttaattacc tgatccacgc
    11341 gcagcttccc gataccggct ggatacgctt tgatggcgtg ttttctccgg aaggacgaat
    11401 tgttctgcgt catctcaaac agcaggagcg ggcgtactga tggcaagtaa cgcgaatttt
    11461 atcagccagt tcgtcatggg cggcgatccc tgtacttata aggaatccgg tgaactacag
    11521 gctgaaatga gtaaactgac tcacccggcc cgacctgatg tggactggcg tcgggtggaa
    11581 aaactcagcc tcgcgctgtt ccggcaaaat ggcgtggaat tacagacgca ggtctgttac
    11641 gtactggcga taaccagacg gcaggggctg gcagggatgg cagacggact cggttcactg
    11701 gacatactgc tccagcgctg ggctgacttc tggccggtac aggtacattc cagaatatca
    11761 ctgctcagtt gggtcacaga aaaaatgcag caggcactga gaacgctgga tattcagtat
    11821 caggatctgc cgcagattta ccgttgtgta cagcatcttt ccgccatcga aaccacgctg
    11881 caacagtgtg aactgtggca tatgacgaaa ctggatgtgc tggccgggca gtttcgtaat
    11941 acggcgctac gtctggagcg gctggcacca cagggagcgg aaaccactat cactccccct
    12001 gaattacccc gccgggaaat gaagcaacca gaaaagtcag aagaaagtcc acagccggtt
    12061 tttgcaacca gacccgctca gcaaaacgat aaggatgcca gtccatccgc gccatcccct
    12121 gaaatctcct ggcagcggac atggccgata tttatggccg gaatggttgt gatggcctgt
    12181 ctcggcggaa caggattatg gggctggtcg cagcttaatc agccggacgc actaatccag
    12241 cgaatacaac tgtctgtcat gccattaccg cagtcgctgg agagcggcga gctggcaaag
    12301 ctggatgtaa aggataaggc gctgctggct caggacagaa caattgcggc aagtcagatg
    12361 caactggagc agttaaacaa attgcctgcc cgctggccac tggagcaggg atatcgccag
    12421 ctacgccagc ttgatgccct gtggccggat aatcctcagg tcagagcgct gaacgcgcag
    12481 tggcgtaaac agcgggagct gagcgccctg tccacagagg cattgaatgg ctacgcacag
    12541 gcgcagagcc agctacagcg cctgtcggcg cagctggatg cactggatga gcgtaagggg
    12601 agatatctga ccggttcgga actaaaaacg gcggtgtacg gcatccggca gtcgttaaag
    12661 gagccgccgc tggaagaact gcttcggcaa ctggaagagc aaaaacagac cggagaggtt
    12721 tcgccgacgc tgttgacgca aattgatacc cggttaaatc agttgctgaa tcgctatgta
    12781 attttactgg atacgaaggt ggaacaaagt cagtaaacgt cgggggattt atgcaccaat
    12841 aagggatatg tgaccttgcc attttttaaa aggagtcttt gtgtacaaaa aagagaggaa
    12901 caggccattc gaaacgcgtg aagaggttct ggcatatcat aagcgggaaa aaataccctg
    12961 tctggagtgt ggcaagttgc tcattttttt accgcgccat atctggtttg tgcatgga
//
LOCUS       DAHBHC010000113        12741 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHBHC010000113 DAHBHC010000000
VERSION     DAHBHC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.114"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..180
                     /locus_tag="M2A35_004413"
     CDS             <1..180
                     /locus_tag="M2A35_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200628.1"
                     /translation="FEGEIAQGFNPSHFARALSAAGMLEAGNDRRYKKKALGRIGGKQ
                     HVFYVLMFQPESEED"
     gene            417..839
                     /locus_tag="M2A35_004414"
     CDS             417..839
                     /locus_tag="M2A35_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7200629.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDGIPF"
     gene            855..1136
                     /locus_tag="M2A35_004415"
     CDS             855..1136
                     /locus_tag="M2A35_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7200630.1"
                     /translation="MIKDSKAEELEAKGLYRRAAARWADVMWLVSTDKEREQVAKRRA
                     ECIRKAARPPVIPDNFGILKEAINRTHTGMGLQKPGGEMFRNYPKKRDN"
     gene            1182..1430
                     /locus_tag="M2A35_004416"
     CDS             1182..1430
                     /locus_tag="M2A35_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200631.1"
                     /translation="MPVTFEEVQQHKKLHDFDDLETTTVKKYRRLLSSDALFFVDHHD
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKQ"
     gene            1705..2874
                     /locus_tag="M2A35_004417"
     CDS             1705..2874
                     /locus_tag="M2A35_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCB7200632.1"
                     /translation="MKKLIELRQQKTALKNQMRSLLEKADSGNRSLNDDEGKQFDELR
                     AKADSLDTEISRLESVADEERSKPGTGIQKLSSDELRNYIVTGDVRSLSTSTDSGRDG
                     GYTVIPELDREVMRQLQDDSVMRVIATVKTAKSNEFQKLVSTGGATVGRGTEGSARSE
                     TNTPKIERVTIKLNPIYAYPKTTQEILDFSEVDILGWLSSEIADTFASTEEDDFVNGD
                     GNGKPKGFMAYTRAATSDKTRAFGTIEKMVAASGTAITADELIDILYKLKAKYRKNAV
                     WVMNSGTAGTLQKLKNENGDYIWRDSLKEGAPDMLLGRPVYCLESMPDIGAGKAPLAV
                     GDFSRGYFIVDHVTGIRTRPDNITEPGFYKVHTDKYLGGGVVDSNAIKILEMKAG"
     gene            2929..3489
                     /locus_tag="M2A35_004418"
     CDS             2929..3489
                     /locus_tag="M2A35_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCB7200633.1"
                     /translation="MKNTDFEIRTSELTASDKKLVGYAVRWNSLSEIIWDEFREQFTP
                     GAFADYLAAGNDVRCLYEHDYTQLLRRTKSGTLVLTEDNTGLRFELTPPDTQLGKDVL
                     TLVERGDITGMSFGFRALCEEWSIAQKPYLRTVTAAELREITITSMPAYPESGVEIAH
                     RSLFAQHPELRPAGNNRHRWAELAGL"
     gene            3491..4714
                     /locus_tag="M2A35_004419"
     CDS             3491..4714
                     /locus_tag="M2A35_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004857970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7200634.1"
                     /translation="MWWPFSRKKSDLRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGRDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPYTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYKQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQEVTISKNSKGGDE"
     gene            4711..5049
                     /locus_tag="M2A35_004420"
     CDS             4711..5049
                     /locus_tag="M2A35_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCB7200635.1"
                     /translation="MIAGNMRNRVTIMTFTTQRLPSGAIQQIWTEGETIWAEVKGISG
                     RELLTAGAETAPATIRVWVRYRGDISAASRLKVLTGAFAGSTLNIIGIPLPDAKRTRL
                     EILAKVDTEK"
     gene            5046..5342
                     /locus_tag="M2A35_004421"
     CDS             5046..5342
                     /locus_tag="M2A35_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCB7200636.1"
                     /translation="MTAEKITLEEAKLHCRIDGNTEDTLIQAYISAALEVCQKHIGRR
                     FDDGLEFTPAIKIGCLMYISQLYEYRTTLGDTDTKEIPLTISALWSVYRDVGVY"
     gene            5342..5782
                     /locus_tag="M2A35_004422"
     CDS             5342..5782
                     /locus_tag="M2A35_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCB7200637.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLKKNPLCVRCLAEGIYMPAFVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYRDQEERAAHRNDWMYGDDD"
     gene            5766..5951
                     /locus_tag="M2A35_004423"
     CDS             5766..5951
                     /locus_tag="M2A35_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200638.1"
                     /translation="MAMMTEQEQNRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            6071..6427
                     /locus_tag="M2A35_004424"
     CDS             6071..6427
                     /locus_tag="M2A35_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCB7200639.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSLYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDHLA"
     gene            6411..8072
                     /locus_tag="M2A35_004425"
     CDS             6411..8072
                     /locus_tag="M2A35_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCB7200640.1"
                     /translation="MTTWHEYAEGVKNGKITACKRLKQAVKRYFSDLENPLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGT
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMADAENFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEDFAFDISDSHKERLAAFDGV"
     gene            8078..8362
                     /locus_tag="M2A35_004426"
     CDS             8078..8362
                     /locus_tag="M2A35_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200641.1"
                     /translation="MSETELLKIIRRVTGISPAAGKREATQPETVTAENYARVVAEVM
                     RRDGIELNGVDMRDIRIRVLELLSYRRRVEMYRESEKKTYQWKKPERLRT"
     gene            9088..9327
                     /locus_tag="M2A35_004427"
     CDS             9088..9327
                     /locus_tag="M2A35_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200642.1"
                     /translation="MQLHVSIFPKQENPFSGEGAIMFVIPVPLKRNTPFRRSWWTHSH
                     IQLTLRVSIADPQTATRQQNVTRFSRSVASLNPLS"
     gene            9850..11214
                     /locus_tag="M2A35_004428"
                     /pseudo
     CDS             9850..11214
                     /locus_tag="M2A35_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11239..11577)
                     /gene="maoP"
                     /locus_tag="M2A35_004429"
     CDS             complement(11239..11577)
                     /gene="maoP"
                     /locus_tag="M2A35_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCB7200643.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            11696..12535
                     /gene="hdfR"
                     /locus_tag="M2A35_004430"
     CDS             11696..12535
                     /gene="hdfR"
                     /locus_tag="M2A35_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCB7200644.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(12631..12706)
                     /locus_tag="M2A35_004431"
     tRNA            complement(12631..12706)
                     /locus_tag="M2A35_004431"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12671..12673),aa:Trp,seq:cca)
BASE COUNT         3394 a         3016 c         3469 g         2862 t
ORIGIN      
        1 tttgagggtg agatagcaca gggatttaat ccgtctcact ttgcccgcgc gttgagtgct
       61 gccggaatgc tggaagcggg taacgatcgc cgttacaaga aaaaggcgct cggcaggatt
      121 ggggggaagc agcatgtttt ttacgtgctg atgttccagc cagaaagcga agaagattaa
      181 ttttttctcg cgtgtagggt gaaaattttg cgggttatgc gggttatttc tgtggataaa
      241 tcagtatcca tatgattatt aaaggaatga cagaaaaatc gtaacccgca aaatacccaa
      301 aataacccgc aaaacggcag ttataacccg caaaagtgcg attataaccc gcagaatttc
      361 accgtaaggg tgtaaacaag cgtaaccatc aacgttaaaa ccggagagca gacagcatga
      421 cagcacagat agcggcttac gggcggctgg tggctgaccc gcagttaaag accaccagca
      481 agggaacaca aatggcgatg gcgagtatgg cggttcccct gccatgcagc caggcagatg
      541 acggaacggc gacgatgtgg ttatccgtcc tggcgtttgg cagacaggcc gacgcactgg
      601 caaaacaccg caaaggcgaa ctggtgagcg tggcgggtaa catgcaggta agccagtgga
      661 caggccagaa cggcgaaacg cggcagggct ggcaggttat cgcagacagc gtaatcagtg
      721 cgcgaacggc gcgaccgggc ggcaaaaaag gccaacaggg gcaggccact gatgcactga
      781 acagggcaaa acaacaggca gggaatgatg atccgtacgg ggacgggata ccgttttaag
      841 cagtgaggta cagcatgatt aaagacagca aagcggaaga actggaggct aaagggctgt
      901 accggagagc ggcggcacgt tgggctgatg ttatgtggct ggtgagcact gacaaggagc
      961 gcgaacaagt ggcaaagcgt cgcgcggaat gtatccgtaa ggcagcacgc ccaccagtca
     1021 taccggataa tttcggaata ctgaaagagg ccataaaccg cacacatacc gggatgggct
     1081 tacagaaacc tggcggtgag atgttcagga actacccgaa aaaaagggat aattgataac
     1141 ggttgttaac tgtttctggt atcgtggcag caggaggaaa catgccagtg acatttgaag
     1201 aagtccagca acataaaaag cttcatgatt ttgatgatct ggaaaccacg acagtaaaaa
     1261 aatatcgccg gctgctttct tccgatgcgt tattttttgt ggatcatcat gattttctgc
     1321 gtagctcact gactggggaa attttcgcaa ccaaccgtga gcaggtggaa gcgatgatcg
     1381 aatatctgtg gaaaataaga cgcagaatgc gggatcccgt gaaacaataa agcgataaag
     1441 gcctggattt tttccgggtc tttttttcag gttttgtaaa ttatttgttc gtggttgttc
     1501 caggttgttc ggtgattctg gctgatgttt acatactgat ttttatgtat atgttggcgt
     1561 gtggcactca gacgtgagcc gccacaatgc cgcctgaccc cctgcgcgat gccgggttga
     1621 tctgcgagat gccgagagtg tcgggcggcg ctccctccgt gttggtttca cgtcctgaat
     1681 cttaaccaat acgagaaaac cttcatgaag aaattaatcg aactccgcca gcaaaaaacc
     1741 gccctgaaaa accagatgcg atccctgctg gaaaaagccg acagtggaaa ccgcagcctg
     1801 aacgatgacg agggcaaaca gtttgatgaa ctgcgtgcaa aagccgattc cctcgacaca
     1861 gaaatttccc gcctcgagtc tgtggctgat gaagaacgca gcaagccagg aacgggcatc
     1921 cagaaattat catctgatga attgcgtaac tatatcgtaa ccggagatgt gcgatcactg
     1981 tccaccagca ctgacagcgg cagggatggc ggatataccg taattcctga gcttgatcgc
     2041 gaagtcatgc gccagctaca ggatgacagt gttatgcgcg tgatcgcgac cgtgaagacc
     2101 gcaaaatcaa atgagtttca gaaactggtt tccactggcg gcgcaactgt aggaagaggc
     2161 acagaaggca gcgcgcgcag cgaaaccaac accccgaaaa ttgaacgcgt aaccatcaag
     2221 ctgaatccga tctacgccta cccgaaaacc acgcaggaaa tcctggattt ttcagaggtg
     2281 gatattctgg gctggttatc ctccgaaatt gccgacacgt tcgccagcac cgaagaggat
     2341 gattttgtta atggcgacgg taacggcaag ccgaaaggat tcatggctta cacccgtgcg
     2401 gcgaccagtg acaaaacccg cgcttttggc accattgaaa aaatggtagc ggcaagtgga
     2461 accgccatta cagcggacga actgatcgac attctctaca agctgaaagc gaaataccgc
     2521 aaaaatgccg tctgggtgat gaactcgggc acggcaggga cactacagaa gctgaaaaat
     2581 gagaacggcg attatatctg gcgcgacagc cttaaagaag gtgcgccgga tatgttgctt
     2641 ggtcgtcctg tttactgcct ggagtccatg ccggacatcg gcgcaggaaa agcaccgctg
     2701 gcggttggcg atttcagtcg tggttatttc atcgttgatc atgtaacagg gattcgcacc
     2761 cgaccggaca acattactga acccggattc tacaaggtcc acacggataa atatctgggc
     2821 ggtggtgtgg tggattcaaa cgccatcaaa attctggaaa tgaaagctgg ctagtcatga
     2881 gtaaggagga ggctgcggcc tcctttttca gctttatgga gtacaccgat gaaaaacacc
     2941 gattttgaaa tccgcacatc tgaactgacc gccagcgata aaaaactggt gggttatgcc
     3001 gttcgctgga acagcctttc agaaattatc tgggacgaat tccgcgaaca gttcacgccg
     3061 ggggcttttg ctgactatct ggcggcgggt aatgatgtgc gctgcctgta tgagcatgac
     3121 tatacccaac tgctgcggcg caccaaatcc ggcactctgg tactgactga ggataacacc
     3181 gggttacgtt ttgagctgac accgccggat acccagcttg gaaaagatgt gcttacgctg
     3241 gtggagcgtg gcgacattac aggaatgagc tttggttttc gcgcattatg cgaggagtgg
     3301 agtatcgcgc aaaaaccgta tttgcgtacc gtaactgccg cagaactccg tgaaatcacg
     3361 ataacgtcga tgcctgctta tcccgaatcc ggcgtggaga ttgcccaccg ttcgttgttt
     3421 gcacagcacc ctgaattacg cccggcagga aataatcgtc atcgctgggc tgaactggcg
     3481 gggttgtgat atgtggtggc cttttagtcg taaaaaaagc gatctgcgta acctgtccat
     3541 tgatgatttt ctggcgctgt ccggtgtacc gaataccgga tccggagaat atgtttctgc
     3601 cggaacggct gaatcattgc ctgcagtgat gaacgcggtt tctgtcattg ctgaggcggt
     3661 ggccacgatg ccgtgttatc tgtatctggt acgtaatgac aagggcaggg aggcgcggga
     3721 atggctggac agtcacccgg tagatattct gctgaatgag cagcctaatt catgccagac
     3781 accttaccag tttaaacgca caatgatgcg tcactgcctg ctgaacggta acgcctatgc
     3841 ggttattgag tgggggcggg atggtcagcc aaaatcactt catccttatg caccgggttg
     3901 tgttgtaccg gaacgcacag gcgcacacaa ataccgctat accatcaccg aaccctatac
     3961 aggaacggtg cgcacgtatt tacaggaaga agttctgcat ctccgctatg cctcggatga
     4021 tggctttctg gggcgctccc ctgtcacgat ttgccgtgag gcacttgggc ttggccttgc
     4081 tcaacagcgg cacggagcca gcattatgaa agatggcatg atggcggcag ggattatcac
     4141 gtcaggcgaa tggctggacg gcgtgaaagg taaacaggca ttggatgctc tggaacgcta
     4201 caagggggcg aaaaatgccg gaaaaacgcc aatccttgaa gggggcatgg attacaagca
     4261 actgggaatg agtaaccagg atgcggaatg gctggcctcc cgtcgcttct ccattgaaga
     4321 catcgcccgc atgttcaacg tatcgccgat ttttctgcag gaatacagca acagcaccta
     4381 cagcaatttc agcgaggcaa gccgcgcgtt tctgaccatg acaatgcgcc catggctggc
     4441 gaacttcgaa cagcaaatca aggccgcttt gctggtggct tctcccgtac ctggtacccg
     4501 ttatctggtt gagtttgatt cagccgattt actgcgcgcc acacctaccg aacgttacgc
     4561 cacgtatgag aaaggaatta agaacgggat catgaatccg aacgaagccc gtgagcgtga
     4621 gggaatgccg ccgcgtgaag gaggcgacga gttcagccag gcatggaagc aggaagtgac
     4681 aatcagcaag aacagtaagg gcggtgacga atgatagccg gaaatatgcg gaaccgggtg
     4741 acgattatga catttaccac acagcgcctg ccgtcaggtg cgatccagca gatatggacg
     4801 gaaggggaaa ccatctgggc agaggtcaag gggatcagcg gacgggaatt actgaccgca
     4861 ggcgcagaaa ccgcacctgc aacgattcgg gtatgggttc gctatcgtgg cgacatatcc
     4921 gccgccagtc gacttaaagt acttaccggt gcttttgccg gaagcacgct taatatcatc
     4981 gggatcccct tacctgatgc gaaaagaacc aggctggaaa ttctggcaaa ggtggataca
     5041 gaaaaatgac agcagaaaaa ataaccctgg aagaggccaa acttcactgc cgcatagatg
     5101 gtaacacaga agacacactg attcaggcat acataagcgc ggcgctggag gtatgccaga
     5161 agcatattgg caggcgtttt gatgacggcc tggaatttac ccctgcaata aaaattggtt
     5221 gcctgatgta catctcgcaa ctgtacgagt accggacaac gcttggcgat accgatacaa
     5281 aagaaattcc cctgacgatt tccgcgctct ggtcggttta tcgtgatgtg ggggtgtact
     5341 gatgccatgg caaccattaa ggcgatgcac tgagccgggc tgtaataagc gcgtgaagtc
     5401 cggcaagtgt gaagagcaca ggcgggctgc atggcgtgca gaggatgcca gacggggaca
     5461 ccgccgcgca cgtgggtatt cccgacagtg ggacaaatat cgcgccctgt acctgaaaaa
     5521 aaatccgtta tgtgtgcgtt gtctggctga gggtatttat atgccagcct ttgtggtgga
     5581 tcacattatc cccatcaatg gcggcggtga tgttctcttc tggcctgagt ggaatcacca
     5641 ggcattgtgc cagacgtgcc acaaccgtaa gacgacacgg gaagatccag ccacgaaagc
     5701 gaaccgtaag gcgggcatgt atcgcgatca ggaagaacgg gcagcacacc gtaacgactg
     5761 gatgtatggc gatgatgact gaacaggagc aaaacaggct gatacgtgga ctgataaggc
     5821 agcgtgacac atggaagaca caggagacag aacacaaagc caacaggaca gggcgcacaa
     5881 aacgcaccac agcgaagcga ttaaccgacc gtgaccgcga ggtcatggaa tgttttcgca
     5941 atcgctggtg aggccgtcag agggggtggg ggaggttttc aggacgaaac cgtccctgcc
     6001 ggacaccgac cgcctcctca aatttttgtg cacgggaatt tttttgaaaa taattgggcg
     6061 aaaaaagaac atggcaagac caccaaaagc ccccgcttac ctggatgaaa tcgcggtcag
     6121 gcagtggaag gaaaaatcgc gccagctttc cgggcgggaa gaccttaccc ccgccgactg
     6181 gagcaatctg gaactgtatt gcgttaacta ctccctatac cgcaaagccg tcgaagacct
     6241 tgcgacacgc ggcttcagca ttgttaacag tcagggcagc gagagcagaa accccgccct
     6301 gagcgcaaag gctgacgcag aaagaataat gatcaaaatg gcttctttgc tgggttttga
     6361 cccggtaagc cgccgcagaa atccaccgga aacagaggaa gaggacgagc ttgaccacct
     6421 ggcatgagta cgcagaaggc gtaaaaaacg gcaaaattac ggcctgtaaa cgactgaaac
     6481 aggccgttaa acggtatttt tctgaccttg aaaaccccct ttacacgttc gatccggagg
     6541 tcgtggagcg gtttattgcc ttttccaggg tgtgtccgca cgtaaaaggc gcaatgcgcg
     6601 gtagccccat tgagctggag ccgtggcagc agttcgcctt tgcctgcatc ctgggcttta
     6661 aggttaaggc caccggacgg cgcaaataca ccagcgcatt cattgaagta ccgcgaaaaa
     6721 atgccaaatc cacggtcgcg gctatcctgg ctaactggtt tctggttatg gaaaacgggc
     6781 agcaggatat ttacaccgcc gccgtgagtc gtgatcaggc gcggatcgtg tttgatgatg
     6841 cgcgtcagat gtgcctttta tcccgaccgt tacgaaagcg ggtaaatatt caggcgcaca
     6901 aggtgataca cccgaaaacc aacagcctgt taaagccact ggcagcaaaa gcggcaacca
     6961 ttgaggggac aaacccgagt ctcgccattg tggatgaata tcacctgcac cctgacaacg
     7021 gggtttattc cgcgcttgaa ctgggaatgg gggcacgccc ggaggggctg ttatttgcca
     7081 tcaccacatc ggggagcaac gttgtttcag cctgtaaaca acactatgac tattgctgcc
     7141 agatactgga tggtgaagag gtgaatgaat ccatgttcgt gctgatttac gagctggatg
     7201 atgaaagcga ggttgacgat ccggcgatgt ggataaaggc gaatcccaat atcgatgttt
     7261 ccgtcgatcg tgaaaaactg gcctcaacca tccagaaagc gcggggtatt ccgtcgcagt
     7321 gggtggagat gcttactaag cgatttaaca tctggtgtca gggggctacg ccatggatgg
     7381 gtaacggtgc atgggcggag tgcgccggaa cgttcgcgga ggcggattta tacgggcagg
     7441 agtgctacgc ggggctggac ttatcatcaa ccagcgatat ttccagcgtg tgctatgcct
     7501 tcccggtcgg taaaaagatt atgctggttt cccgtcacta tctgccggaa tttcagctac
     7561 agaaccctgc caataaaaac cgcgccatct atcgccagtg ggcaaaggcg ggctggatac
     7621 gcacaacacc gggtgactgc attgattatg accgtatccg tgatgacatc atggcggatg
     7681 cagagaattt caatatcagg ctggtgggtt tcgatacatg gaacgccacg cacctgagga
     7741 cgcagctaca gggcgcggga tttgaggtgg agccgttccc gcaaacatac cttcgtttca
     7801 gtccggcggc gaaatcgttc gaagtttttg ttaaccggaa ggtgattgtg catcgtggtg
     7861 atccggtgct ggcctggtca atgagtaatg ttgtgatgca gagtgacgcg aacgccaata
     7921 tcaagccgaa caagaaaaaa tcatccaaca agatagaccc gagcgttgcg gcgctgatgg
     7981 cgtttggcac attccaggca gagcatgaag attttgcctt tgatataagc gacagccaca
     8041 aagagcggct tgcggcgttt gatggggtat gacgagaatg agcgaaaccg aactactaaa
     8101 aataatccgc cgcgttaccg gaatcagccc ggcagcaggc aaacgtgagg ccacgcagcc
     8161 ggaaactgtc accgctgaaa attacgcgcg tgtggtggct gaggtgatgc gccgtgatgg
     8221 tattgagctt aacggcgtgg atatgcgcga catacggatc agagttctgg agttgttgtc
     8281 atatcgtcgc cgcgtggaga tgtatcggga gagcgagaaa aagacttacc agtggaagaa
     8341 accggagcgg ctgcgtacgt aatacgtacg gacatcgaga caaagacgag cagcattctg
     8401 gttcagtgca aaacaacgta aacgtggttt tgcgtcctga gtcatcgtaa tggttttatg
     8461 cgttctgtgt tgcgctggtg gatttgaggg atatagctgg tagtggtaaa tcccttttgc
     8521 gtggcggtgc gatggctgca tggtggaaat caccacgaag gggaggtgat aaacactccc
     8581 ccttttcgtg tggtgcatgg tcgaaatgac cacggaagag acgcgcaccg aggcagaacg
     8641 attaccgtaa gttaaagtta cagtattcag tttttacaaa gcactttttc gggctttggc
     8701 ctaacccact gatattaaag cagagtataa atttacattc tggtgctgta attatctgat
     8761 ttacccggtg gtttgtcttg tcagaccata gccgttaacg taactggttg atattttcga
     8821 aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgattctt ccagaatccc
     8881 ccattggggg aggctgggcg gcctgctgtg ggcttttcca ccattggtgg aaaagtggcg
     8941 cgactacagg gctggatcat caatatggtg acgcagcaac agagtgacca ttagtcacct
     9001 tgttctaact cgctgtattc gtttaatttc caccaactgc ggaaattgag aaactcaccc
     9061 gccagaccct gaatagtgaa aatcgctatg cagcttcatg tgtccatttt cccgaaacag
     9121 gaaaacccgt tttcgggaga aggtgcgatc atgttcgtca tacctgttcc gttgaaacgg
     9181 aataccccat tccggcgtag ctggtggacg cacagccaca tacaactaac tttgcgcgtc
     9241 agtattgctg acccgcaaac tgcaacccgg caacagaacg ttacccgatt cagccgttcg
     9301 gtggcatcac tgaacccact ttcctgatgc gtttgcgggt tatttggggg gtgtttgcgg
     9361 gttattttga atgtcttgcg ggttacgttt tggctaacat ttaaacaccc catttttcaa
     9421 cacattgata ttaaagaata aaaaatactt agcacgcgaa gataacccgc taacccgcat
     9481 aacccgcact gttttgtata tatatatata cgaaaaattg cattcaggag ggggatcgaa
     9541 attcctgcaa tccattactt atggaactat ccgccccgtc aaatttttat acccgcgaaa
     9601 ttaaaaaaat gcgtcgcgct ggtgggcatg atgccgatat gggaattccc ataacgacca
     9661 accccatttg aaactatgca aatctgtata gaaaaatcat cccttcctgt atatcgcttg
     9721 tgtctatttg ttccacgttg ttccatacag tgcaccgaac ggtgtagtta atggtgtagt
     9781 caatttgcga tttataacgt catatgcatc aagatttatc atttaaattc aatatgttgg
     9841 gtttatttga ttataaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg
     9901 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
     9961 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10021 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtgc aactgaagct
    10081 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10141 aacggtgaag gatgcctgat agccgatcat ctgcaggctg tctgtgcgtt tctggaaggt
    10201 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10261 ctcagtgatg ttgtcggtca ggagcaagga aagcgaggac tggaaattac cgccgctggc
    10321 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    10381 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    10441 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcaccg
    10501 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    10561 atttcgttgg cgcataacgg cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt
    10621 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacacga
    10681 gccaaaataa cctatccagc ccgtttccag cttgttgcgg cgatgaatcc cagccctacc
    10741 ggacattatc agggaaacca taaccgctgc acgccagaac agacattacg ttatctcaac
    10801 cggctctcgg ggccctttct cgaccgcttc gatctctcac tggagatccc attaccaccc
    10861 cccggcattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc
    10921 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaatgc ctggctggat
    10981 agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gttggaagga
    11041 acactgatcc atctggggtt atcgattcgt gcctggcagc ggttattgaa agttgcacga
    11101 accattgctg atattgatca gtctgacatt atcacacgtc agcatttgca ggaggcagtt
    11161 agctatcgtg cgattgaccg tttgctcatc catctgcaaa aactactgac ataaaaaaag
    11221 ggcatttcgc cctttttatt aatcatcaga atcggtgtag tcttcagcac cttcaacctg
    11281 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt
    11341 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    11401 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    11461 accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc
    11521 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    11581 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    11641 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    11701 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc
    11761 tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca
    11821 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga
    11881 aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc gcaaggaggt
    11941 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg
    12001 tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca
    12061 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    12121 tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg
    12181 atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta
    12241 tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    12301 cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct
    12361 taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt
    12421 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    12481 aaatacgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga
    12541 atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa
    12601 aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa
    12661 cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt agggtggcgg
    12721 aacggacggg actcgaaccc g
//
LOCUS       DAHBHC010000114        12141 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHBHC010000114 DAHBHC010000000
VERSION     DAHBHC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.115"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..576)
                     /locus_tag="M2A35_004432"
     CDS             complement(<1..576)
                     /locus_tag="M2A35_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCB7200645.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEVDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VARRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAIADE"
     gene            complement(576..917)
                     /locus_tag="M2A35_004433"
     CDS             complement(576..917)
                     /locus_tag="M2A35_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7200646.1"
                     /translation="MPESEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGGLNADL
                     KTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISDNAMYT
                     GFNCTFEEVTH"
     gene            complement(910..4149)
                     /locus_tag="M2A35_004434"
     CDS             complement(910..4149)
                     /locus_tag="M2A35_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7200647.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQASQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNENGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGIAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLAAAQASLTRNIAART
                     AAQTTLNTVTSVGSRLLSGALGLVGGVPGLVMLGAAAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALKEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQTALNNSKRELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLDAW
                     RNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDDAQLLGYGEGSRMRER
                     LQEMWSIRQAFEQKNNELLRQYQAGEIEEALWKQEKSLNEKYLEERLSDQQDYYAKAD
                     ALRSDWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSILQEVSKILMNAAIVNGLKSLSKSMSGAGGWLGTVGDWLSGAVANAKGGVYTSANL
                     SAYSNTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVY
                     SPVYHISIQNKGSNGEIDTQSARGLVELIDSRVVSIMQSSRRDGGLCSA"
     gene            complement(4195..4473)
                     /locus_tag="M2A35_004435"
     CDS             complement(4195..4473)
                     /locus_tag="M2A35_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCB7200648.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEQEQTEGASDPFAGLENALLIVSQ"
     gene            complement(4497..4868)
                     /locus_tag="M2A35_004436"
     CDS             complement(4497..4868)
                     /locus_tag="M2A35_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCB7200649.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(4883..5587)
                     /locus_tag="M2A35_004437"
     CDS             complement(4883..5587)
                     /locus_tag="M2A35_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCB7200650.1"
                     /translation="MSSNFERSQQTKVMISSAPVTAETLSSASFLELSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTEPLKVKVDLNSTLQVS
                     AGAKLEMVVEAAGGVPPYSYVWKKGGSPVSGQTAATFSKASAVSGDAGAYTCEISDSA
                     SPVSKVTSTSCAVTVS"
     gene            complement(5648..5992)
                     /locus_tag="M2A35_004438"
     CDS             complement(5648..5992)
                     /locus_tag="M2A35_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCB7200651.1"
                     /translation="MRETTLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADDIREQALDAVRQLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(5989..6435)
                     /locus_tag="M2A35_004439"
     CDS             complement(5989..6435)
                     /locus_tag="M2A35_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCB7200652.1"
                     /translation="MDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDAV
                     VERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAFY
                     WRFVELGTINMPAHPFIRPAFDTTEELAAQIAIQRMNQAIDEVLSK"
     gene            complement(6435..6773)
                     /locus_tag="M2A35_004440"
     CDS             complement(6435..6773)
                     /locus_tag="M2A35_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCB7200653.1"
                     /translation="MQAGRLRDRVVILNATTVRSPSGHPVETMTEGATIWAEVKGISG
                     RERISGGAETAQATVRVWMRFRRDVTATSCLKVLTGAFRGAILSIDGPPIPDARATRL
                     EILCSQKGNV"
     gene            complement(6782..7087)
                     /locus_tag="M2A35_004441"
     CDS             complement(6782..7087)
                     /locus_tag="M2A35_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCB7200654.1"
                     /translation="MMPTLEELRVQCRIDDDNEQENSLLMMYLAAAREEAEKFLNRTL
                     YDETVSEQDTTGLVITPLIKLRLMQLVGYWYENREMQDAVPDFFYTGLRMYRFHPGT"
     gene            complement(7099..7287)
                     /locus_tag="M2A35_004442"
     CDS             complement(7099..7287)
                     /locus_tag="M2A35_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200655.1"
                     /translation="MILKQDLKWSPDGMRVEIIRAGEYEDKELPERVREIATAAGIVS
                     DKRTPVARGADKSKKQHS"
     gene            complement(7338..8543)
                     /locus_tag="M2A35_004443"
     CDS             complement(7338..8543)
                     /locus_tag="M2A35_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCB7200656.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVEAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGDATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(8558..9208)
                     /locus_tag="M2A35_004444"
     CDS             complement(8558..9208)
                     /locus_tag="M2A35_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCB7200657.1"
                     /translation="MQTKQRLDVPLSLKFVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(9186..10427)
                     /locus_tag="M2A35_004445"
     CDS             complement(9186..10427)
                     /locus_tag="M2A35_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7200658.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPVAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(10427..10609)
                     /locus_tag="M2A35_004446"
     CDS             complement(10427..10609)
                     /locus_tag="M2A35_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200659.1"
                     /translation="MFLIILAPLVGVLGALLLAYGAWLIYPPAGFVVAGVLCLLWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(10621..>12141)
                     /locus_tag="M2A35_004447"
     CDS             complement(10621..>12141)
                     /locus_tag="M2A35_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCB7200660.1"
                     /translation="VFILINIFGFVIPLVNEETGEVVMRSDGSGRPVMVRRFRTAYNE
                     VARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRDQARIVFEDAKNMVRKARSTLG
                     RLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCAIIDELHAHKTRDVWDVLETAT
                     GARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYNSDVEGAVKDDSYFAIIYTLDE
                     GDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQVSARVNFFTKHMNVWVTAESAW
                     MDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICAAAKLWRTDNGHVHADFKFWLP
                     EGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQIKSDLLEWIGGENLRELGFDPW
                     SAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLVYAGRFHHSNHPVMNWMMSNVT
                     VKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNGGEPEPDLSEHLVSVGIRSL"
BASE COUNT         2719 a         3613 c         2812 g         2997 t
ORIGIN      
        1 ttcatccgcg atcgccggac cgtgataacc gcactcatcg ccgcgatagg tccaggtgca
       61 ggtgttggcc agcatgatac gtcccggaaa aacagcgcca tccgtttccg tcggtgtgga
      121 caacacaaag gaggcgctga ccgcactcag ttcgctgcac tgctcgatgc gccagcggct
      181 gatcacctcc tgctccggat cagcttcgct gtttccgttg acgaagttca ccgcatccag
      241 aaaacgggcg taaaccttac gcctggccac cgttccgccg accagactct gcaggtcttc
      301 cgccatcccg gtgaccatgc cgtgcagatt agagactttc agcgttggtc ttgcactggc
      361 tcctttgccg ttcatctcaa agccgcttcc ctgaatgggg tacgcctgat actgccgccc
      421 ctgccagatg accggctcac ctttttcgtt ctgctcatta caaaaaaaat aacgctcgcc
      481 accgacctcc gtcagatcga cttcccagag cacgaccagc gcggattgct ccgttttagt
      541 gcactcattg agtgtttcct gctgtatatc ctgcatcagt gagtgacctc ttcaaaggta
      601 cagttaaaac cggtatacat ggcattatcc gaaatgctcc actccctgca gacaacccgg
      661 acagtcctgt tgtgttttgg cggacgccac aaaaaagcac gaatcccggc atgacgggat
      721 aaaaaactgt ccagcgcggc acgggaatat tcatctgtga cacgaaatac cggtttaaac
      781 gttttcagat ctgcattcag accaccagcc cgtcgctgtt catatccgtc accgaacttt
      841 accgtaataa cagatggctt tcgtgtcgtc tccatcccct cgcgggggat ccagttaaaa
      901 acttcagact caggcactgc ataatcctcc gtcccgacgt gatgactgca taattgacac
      961 aaccctgctg tcgatcagtt ccaccagccc cctggctgac tgcgtatcta tctcgccatt
     1021 gctcccttta ttctgaatac tgatgtgata cacgggagaa taaacaaatc caccgccacc
     1081 attcacattg ccaatggctc tgactccaag agagccgtcc gctgcccggg tcagtggcat
     1141 gatagcttca ggccctgcct cgcccatcag cccggcacct ttcgcaaaag caaaatacgt
     1201 cggggtatcc acaatggtgt tactgtaagc actcagattt gccgatgtat aaacaccacc
     1261 ttttgcgttt gccactgcac cggaaagcca gtcgccgacc gtaccaagcc atcctccggc
     1321 accggacatg cttttggaaa gtgacttcag cccgttaacg atggcagcgt tcatcagaat
     1381 ttttgaaact tcctggagaa ttgaactccc ccagtttcgc cagtccacaa catttccggc
     1441 cagtgcatcg gaaatatttg ataccagccc gtccatagtg gaaacgacag catctgccgc
     1501 ctgcgaagca taatcggtgg cactgtctgc ccagttcgtc agcccctcct ggagtccggc
     1561 attccagtca ctgcgtaaag catcagcctt tgcataataa tcctgctgat cgctgagacg
     1621 ctcttccaga tatttttcat taagcgattt ttcctgtttc cacagggctt cttcaatttc
     1681 tccggcctga tactgtctca gcaactcgtt atttttctgc tcaaacgcct gccggatact
     1741 ccacatttcc tggagtcgtt cacgcatccg tgagccttca ccatatccca gcaactgcgc
     1801 gtcgtcagat gcccgggcac tggcattact gtccgccaga ctgctctcat acgcagcaag
     1861 ctgctcacga atctttttct ggtcgatgag tgctgcattc tgcaaaagcg tttttttctg
     1921 cgcttctgac agggttgata attcgccctg actgacctga tatttcatct tagccagttc
     1981 agtattctgc cctgccagtg ctatctgctc tttctgctgt ttaatcagcc gtttataaat
     2041 atcttctgtt ttttccgctt cggtcttttt atgcgctttg ggtttatttg cctggttatt
     2101 tcgccaggca tccagtgagt tattgatata attctgtctg gctgtctgat acgcctctcc
     2161 cacaaagccg agatcatccg cagcatagcc cagtcgggca cgctcacgcg cttccccctt
     2221 aaggcgggac agagccagtt cgcgcttgct gttattcagt gcggtctgct gtttatcatc
     2281 cagggttgcc tgtggtagcc gtaacggtac attcaccagc ccctgtcgct gctgaagtaa
     2341 ttcattaccg agcccgagaa ggcgattaaa ctcggtatgc tgcccattca tgatcaacag
     2401 ggactgatac gctttgtttt gttccgcggc ctgttgacgg atcaacgcca cccgtcgctc
     2461 ctccagcccg gcaagcacat cctgaatgga ttgcgctttg ccctgcattt gtgtgagacg
     2521 ggactgttca actgccagtt gatttgttgc ttctgcaagc ccttctgtaa cagtttttac
     2581 cgacgtcatg tggttaatca taaaaccgtt atcggttgtc cagcccgggt ttgccagcac
     2641 atactgatag ccagcaattt tttcctgtaa ggatttaatc ttacttttct gctcgtcaat
     2701 taacctgttc tgctccttca gtgcctgtcg cgtcttttcc tcattatctg acgcttcagg
     2761 aagcgacatt gccgacgttt tctggcgaat ttcgtcgatt gttgcggcat actggcgtgc
     2821 agattctctg gcctgctcct gattctgata catcgtgtac caggccgcag cccccagcat
     2881 gacaagcccc ggcacaccac caaccaaccc cagcgcacca cttaacagac gactccccac
     2941 tgacgtgaca gtattcagcg ttgtctgtgc cgctgttctg gccgcaatat tacgggtaag
     3001 tgatgcctgg gcagcagcca gtttcgcttc tgctgcggcc tgcctttcgg taccgcgagc
     3061 agcaacaacc gcctgttgcg cacgataaac cgccgcacgc gccctggcgg ttgctatctg
     3121 tgtcccccga agttgcgctt cagcaagagc cacttcgttt ctggctgcag taattaatcc
     3181 ggcagttgca gatccagcag acgacgccat attgccaaaa tatcgggcta ccccgacggc
     3241 aaccagagca ccggcagcgg ttgccacggt gtcaatattg cctgcaatac cattcagcac
     3301 accggagagc gtcttcgtca ctccgcttgc ctcgttcgca ccaccaaccc aggccataaa
     3361 ggcgttttca actttggttg cagaggatga aaccgtatca ggcattgccg catattcatc
     3421 acgtaatgcc ccaagctgac taatcagtgc aggaacaacc ttatcggcgg tcagttttcc
     3481 gttatccgcc atggccttca gatccttacg ggcaaccccc attcccgcag ccagcgcacg
     3541 aataacacga tcgccgttct cattcaccga gttaaactct tcaccgcgca gcactccctg
     3601 cgccagtgcc tgactgaact gcgtgatcac tgaactggct tctgctgtac tggcaccgga
     3661 taatttcagg cctgtggaga tcgcctcggt gactttcagt acctcctcag aactgtaacc
     3721 atactcccgc atggaagctg cagaacgggc aaaaaggctg gcgttatcag aaaacgccgt
     3781 ccccgttctc tggctgatcg ccattaattc acgctgtgat gcctgaaaat catcactgga
     3841 ctgtgaggcc tgcttcagac gtgcatttac tgaattccac tcatcggcga gagaaataag
     3901 atgaccggta gcaaaagccc cggcaaatgc ccccgccata ttcagtgccg aagatttagc
     3961 tgtatttatc tgatccgtca cttctgccag tgcacgccgc atttcacggg atgcagcagc
     4021 ggactgtcgg cctccgttct gcatggtgcg gtagtaatcc tgccccatac gcgaagcccg
     4081 ggagatctct gactggaatg accgggaatt tgccgagatt ttaataatca gttcacgtaa
     4141 tgtcgccaca ctcattctcc ggacgaaaaa aaccgccgaa gcggttatgt tgactcactg
     4201 agacactatt aaaagcgcgt tttccagtcc ggcaaatgga tctgaggcgc cttctgtctg
     4261 ctcttgttcc cactgaagaa gcgcatcatt cagtggtact ttgacaccct gcgcaccgta
     4321 aaccgcagaa actatctggg cagcccggat atcagcccgc tcatcaccca gcgggctgaa
     4381 cctgtcaaat tctgcccaca tcatgatttc tgatgcggac atttcccggc gtaactctga
     4441 caatgtgcgc cccatcctga gcgccagcat catcagaaaa cgcatccccg gaagcgctac
     4501 ttttttttaa cctcgccggc atcactgatc agttccagag actgccgaag aagccgcgca
     4561 tgcaccgggc catacacggc aatcacctgt tcacgatcat cctctgaaaa tacaggttgc
     4621 agtccggtat cacacagaac atcaatgaac agttcaacat ctgcctccag atttcggcgg
     4681 gcgcgctccg caacggataa cggtgtctca tcatcttttg ctttaacgat ctcctgccag
     4741 cgcaaccagg cttctgcaga aggttcccgt aatacaaccg ttgccccttc ccattcaggc
     4801 acatcaacgg ttttatggcg aaaccccgac atcgttgcca gtgccagatt acggatattt
     4861 ttagtcatca catctatcct cattaactga cggtaacagc gcaggaagtg gaggtcacct
     4921 tgctaaccgg gcttgctgaa tcagaaatct cgcaggtata cgcaccggca tcaccggata
     4981 ctgcggatgc cttactgaac gttgccgccg tctgtccgga aacaggagaa ccacctttct
     5041 tccagacata agaataaggc ggcacaccac cggcagcctc aaccaccatt tcgagtttcg
     5101 caccggcaga aacctgcagc gtgctgttta aatcgacctt cactttcagc ggctctgtcg
     5161 tcagcacagg tttacctttc aggcgcaggg aaaacgttgc agccacaaca ccattggttc
     5221 ctgcagacca ggtatgctga cgcacctctg ccataaaggt aaatccgttg cctgacggaa
     5281 aaataacttt aaagccatac gtggtgtcat tgtcataggc actgcgcaac gcgttctggg
     5341 cagcattcag ataaaagttg cctgacatgg aaatctctga cgcggcacca agaccgttaa
     5401 tattttcctg ctcaacagaa cacagcgtgg tgacatcaat atcctgtttc tgcccggcgg
     5461 taaactgaac ctctttaatc gtacagctca gttcaagaaa actggcagaa ctcagcgttt
     5521 ctgccgttac cggtgcagac gagatcatga ctttggtctg ctgagaacgt tcaaaattag
     5581 aggacatact cgtctcctga aaataaaaaa acccgccagc ggcgggtggg taaaatcatt
     5641 aacgacctca ggctattacc tgaaattcaa gcgtggctct gctcagacgg gaatcaggat
     5701 cataaccctg cgttttagaa ataacggagg gtgccagttg ccttaccgca tcaagcgcct
     5761 gctcacggat atcatctgcg tcatcaggta ctgtcgccca gacatcgatc tgcacggtaa
     5821 ttcttgattc agcctgacca tcaagcacat cagatgccgt gtcagacacc acagaaaaca
     5881 ccagccatgg cggagatacc gcaggctttc cctccgtcag cgggaccaca taaggataaa
     5941 cctgtcctcc ggccagttga gacagcaggg aatacagtgt ggtctctctc atttacttaa
     6001 gacctcatca atagcctgat tcattcgctg tatggcaatc tgtgctgcca gttcctctgt
     6061 cgtatcgaaa gccgggcgaa tgaacggatg cgcgggcatg tttatcgttc ccagctccac
     6121 aaagcgccag taaaacgcat ttcggggatc gctggctttc atgctgttat cactgtttcc
     6181 ggttcgcagg ttccgtccgc gaatgtggac acccgagata atttcccccc gacgctttga
     6241 acgctgagtg agaacaacca catttttttt cagttttccg gttcgctccg gcgcacgttc
     6301 aacaactgca tcccgcataa cttcagcacc ggcacgggtg gcatcgcgca gaaccttatt
     6361 gttttctgcc ctgctgagcg tctccagatc ccgtgcaata tccgccagac ctgaaaaatc
     6421 aagactgaaa tccatcacac attccccttc tgagaacaga gtatctcaag ccgggtggca
     6481 cgggcatccg gtatcggcgg accgtctata ctcagaatcg cgcctctgaa tgcaccagtc
     6541 agcactttca gacatgaagt tgctgtcaca tctcgccgga atctcatcca gaccctcact
     6601 gtagcctgag cagtttctgc gcctccggat attctctccc tgccactgat ccccttaact
     6661 tctgcccata tggttgcccc ttccgtcatt gtttccacag ggtgccctga tggagaccga
     6721 acggtggtgg cattcagaat aaccacacga tcacgtaatc ttcctgcctg catgaatcct
     6781 cctatgtccc gggatgaaac cgatacatcc gcagtccggt atagaaaaaa tcaggcactg
     6841 catcctgcat ttccctgttc tcgtaccagt agccaaccag ttgcataaga cgcagtttta
     6901 tcagaggtgt tattacaagc ccggtcgtat cctgctcaga aacagtttca tcgtaaagcg
     6961 tccggtttaa aaacttttca gcctcttccc tggcagcagc cagatacatc ataagaagag
     7021 aattctcctg ttcattgtca tcatcaatcc ggcactgaac acgaagctct tccagagtgg
     7081 gcatcatttg ggcaacctct atgaatgctg ttttttagac ttatcagccc cccgcgcaac
     7141 aggtgttctc ttatcagaga caatcccagc tgcagtggca atttcgcgta cccgttcggg
     7201 taattcttta tcttcatact caccggcccg aataatctca acacgcatac cgtccggtga
     7261 ccatttcaga tcttgtttca ggatcatgat tctttcacct gtcagaacag gggcgcactt
     7321 ctgcgccccc tgaatgatta cgccgctgca atcttcagca gtttgatggc ctgcgaatcg
     7381 accagcatgc cgccggtgcg cttggtggta taaaaaccga caaacggttt attggtgtac
     7441 gggtcacgca gaatgcgggt accgatacgg tcaacgatgg tgtaaccccg tttgaagtta
     7501 ccaaatgcaa tggctttcgc atcagcggcg atatccggca tctgttcgtt ttcagcgata
     7561 ccgtaacccg ccagagagga cggctgcccc agctccagcc ccggacgcca cagatagtta
     7621 ccctcgctgt ctttcagcag acggatggca aacaggctgt tgttgttcat catgaacttc
     7681 gcgccggtgc ggtgtgcctt acgcagcgtg taaatcagtt tgataatggc gtctgcggtc
     7741 accgccgtcg cgtcgccgga tacaatatgc tgaagtttgc cgaacgcccg gaccttatcg
     7801 gtttcatccg tggattcata cgccaggaac cctttcggct tcttggtacc atcgccggtg
     7861 gtaaaggcaa tttcttcctg ttcggcaaat tcggttgcca gctcgctgtt gatccaggcc
     7921 tccacgttga aaaaggcatc atccagcatt ttctgggtgg cctgcgggtt accgtagatt
     7981 tcccccatga aaggttcaat caggcccagt tttgaggtgg cagtctggga gcgcgcgtca
     8041 gtctcgccaa cccatccgga agccgtaccg cccagattca ccagtttttt gtagtcggaa
     8101 ccgccaacgg tgatcaccgt ggcttcctgg cgcatcacca cttcatcttt cagcagggtg
     8161 agaatgttgc gatccagtgc ttccggcacg gcatagccac cgtcttcatc ggtgcccacc
     8221 tgcaatgcct tacgctccag atcgcgcaga ccgtcttcac ggcctttacg caggaagccc
     8281 acaaacgcct ctttatgctc ggtggccagt ttattttgcg caccacctgc cggacgtttc
     8341 agctcaagca gctctttttc aaggtcgctt ttgagatttt ccagctcgct gagttttccg
     8401 ttcagggttt ccacctgccc ggcaagtttg cctttttcct gctcaatcgc ctccacgcgc
     8461 ttgtcgttct ttgctttgaa gtcgtcaaac ttctgctgca gctcctgcgc gacctgttcg
     8521 acatctttaa tatcaaccgc catcgtattt ctcctgatta gaagttcaga tttttcagtg
     8581 cattcagtgc agagcccaca tcctcagcgt cgcgcaggga cagtgcgcca tagcccccgg
     8641 ccatgaatgc tttggcctgg gtacgggaga gtccgacatc acgcaggact ctttcgattt
     8701 ttttctgttc gggaatttcc ccgcgggcca gtgcgttctt gacgtcgctg atccgcgcct
     8761 cgtcgttaga cgggaacgtc accaggctga cttcccagag gtcgatttct ttcagcagaa
     8821 aggcttcttt gctccggtcg tattcccagt ctttcaggac gtacccaata gaaaggccgg
     8881 ttaacgaacc ggccttcatg tgtgcatgtg cgcgttttgc gaggggatca tcatcaataa
     8941 gcaaccgtcc cctgacgtaa agcccgacat cgtcttcctt catttcggtg taaacaccga
     9001 tgggttcatc catgcggtgc tgccagagca gcgcaggtaa cgcttttctg tcactccacg
     9061 cccgcaggga agcagcaaat gccccggaca tcaccacatc atcgtggctg tcctttacac
     9121 caaagacgga gccataccct tcaaactcac cggagtcact gacaaatttc agactcagcg
     9181 gtacatcaag acgttgtttc gtctgcattg gcgttatcct tctgcttacc ggctttactg
     9241 ccatcggagg gtttcgtggt catgttcatc ggtgtgagat agacatcacc accgggacgc
     9301 ggattcatat cttccaggtc gcggcagtca ttgggagagt aaattcccca gttaatcccg
     9361 gtggcgtagg cttcaaaacg ggacttcata tccccgcgca gtaacgcccc ggcgttaaat
     9421 ttggcgtaat aaacgccctg cttacttttt cgtaccagtc cggtgttgat ccgctgttcg
     9481 atgcgggtca gatacggcac cagtgaatag ttgataaatc cgagccccag ctcttcgata
     9541 ttgttgaagg tggcgcgatc ggtgttctgc accatgtgca acggcacccg gaacaaacga
     9601 cagatttctt caagctgaaa cttgcgggtt tccagaaact ggctgtcctc ggcgttcagc
     9661 gccatcgact tccagtccag ccccatctca aggatcatcg ggcggtgagc attaccaagc
     9721 ccggtgtgac gctcctcaaa atctttcttc aggcgctcat aagcctgatc tgacagcgtc
     9781 tgttctgtac gcaacacacc ggacgtcacc gcaccattgc tgaacagcct ggccccgtgc
     9841 tcttcggtcg ccgctgccag cgatattgcc tcgcgggcat aggcgacggg attcagcccc
     9901 accagaccgt ccagcgtcag cgtgcgcaca tgccagatat cctcctgggt cagcacatcc
     9961 gtggaaccgt ccgggaatgt gacctgatag accggctccc agctactgtt aagcttcggc
    10021 accacacagc caggatcgac gggcagcagt tcagccactt cgccaaatgc tttcactttg
    10081 taagcataaa agtttccccg caggcacaga caggtgacca ccagctccca gaactcctgc
    10141 ggcgtcatat agccattggg atgcgtggag atcagcttat gcagacgttc gccggtggct
    10201 ctctgtttca ggctgccgtt caggtgatac agattgcagg gcaacatccc gaccgactct
    10261 gccagcaccc tgacgcagga aaaaaccgcc gtcagtcgca tggcccgctg gctgctgatc
    10321 tgctttccgg tataggtgtc atatgacagc ccgatagcat ccgccagttc tgctggcgtg
    10381 gtcaccggcg cgtcactttt tcgttgaaat aatcccgaaa agaacactat ttacctccgc
    10441 cgacagacga ctgtgtacgg tcgagatatc gcgccaccag ccacgaccag agcaggcaca
    10501 gcaccccggc aacaacaaaa cccgccgggg gataaatcag ccaggcacca tacgccagca
    10561 aaagcgcacc cagcacgccc accagtggcg cgagaattat cagaaacata atgacctcgg
    10621 ttaaagtgag cggatgccca cgctgaccag atgttcagac agatccggct ccggttcacc
    10681 accattgacc agcatccggc tcatcgctgt aaacatcgca acagggccgt cgattttggc
    10741 ttccggcgtg gatttattcg ggaagatatt gtcgtttttg tccggtttta ccgtaacgtt
    10801 agacatcatc cagttcatga ccggatgatt gctgtgatgg aaacgcccgg catagaccag
    10861 tgattccgtt tccttcatgg cctctgacag attgcggacc gtctgcggaa cctccaccag
    10921 tggtatccct tcttcagcca gtgccaggct gaactgcatc gcgctccacg ggtcaaatcc
    10981 cagttccctg aggttttcac caccaatcca ttccagtaag tcacttttta tctgagcatg
    11041 atcgataaca tcaccatccg tcagaatcag cttatccatc tccgcccact tccggtaaag
    11101 ttctgcctgc tgccgcgagc atcgttccag ccgtccttcc gggagccaga atttaaaatc
    11161 ggcatgaaca tgcccgttat ccgttcgcca gagttttgcc gccgcacaga tatcaatctt
    11221 atgagcaagg tcaacgccga cccacatggg atacgttttc agctcatgtc gtggggcaat
    11281 atattcgcat ttctcccact taatcatgtc catccaggca gactcggcag tgacccacac
    11341 attcatgtgt tttgtgaaaa aattcacccg cgcagagacc tgctccttcg ccttttttgc
    11401 cagacgacgc agatcatccc agcgtttaca gatgcccagg ccgggattcg ctttctgcca
    11461 gaccgtttca tcaaacggat catctccctc atcgagggtg taaataatcg caaagtagga
    11521 gtcgtctttt accgcgccct ccacgtcgct gttatagcct cgcaatacct tgatggcgta
    11581 atcgcgttgc tcgtaacaaa tcccttcctt gttaaagcca gccgtggtga taccaaataa
    11641 aagggactgc agacgggcac cagttgccgt ttccagaacg tcccacacgt cgcgggtttt
    11701 atgtgcatgc agctcatcaa taatggcgca gtggatgttc agaccatcca ggttgtttgc
    11761 atccgaagaa agcggttcaa attttgatgc gctctgctcc tggtaaatcg ccagcttgtt
    11821 gaaatcaaac aaccgcccga gtgtcgaccg ggcttttctg accatatttt tggcgtcttc
    11881 aaacacgatt ctggcctggt cacgcgtggt tgcggctgaa tacacctcag ccccgccttc
    11941 accatctgcc cccgtcatat acagaccgat acccgatgac agggttgatt ttgcgttttt
    12001 acgggcaact tcgttgtatg ctgtccggaa ccggcgcacc atcaccgggc gcccgctgcc
    12061 atcgctgcgc atgacaactt ccccggtctc ttcattcacc agcggaatga caaaaccaaa
    12121 aatattaatg aggataaata c
//
LOCUS       DAHBHC010000115        12051 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHBHC010000115 DAHBHC010000000
VERSION     DAHBHC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.116"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(229..2802)
                     /gene="clpB"
                     /locus_tag="M2A35_004448"
     CDS             complement(229..2802)
                     /gene="clpB"
                     /locus_tag="M2A35_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCB7200661.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2932..3663)
                     /gene="pgeF"
                     /locus_tag="M2A35_004449"
     CDS             complement(2932..3663)
                     /gene="pgeF"
                     /locus_tag="M2A35_004449"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCB7200662.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3660..4640)
                     /gene="rluD"
                     /locus_tag="M2A35_004450"
     CDS             complement(3660..4640)
                     /gene="rluD"
                     /locus_tag="M2A35_004450"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCB7200663.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4775..5512
                     /gene="bamD"
                     /locus_tag="M2A35_004451"
     CDS             4775..5512
                     /gene="bamD"
                     /locus_tag="M2A35_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCB7200664.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5783..6124
                     /gene="raiA"
                     /locus_tag="M2A35_004452"
     CDS             5783..6124
                     /gene="raiA"
                     /locus_tag="M2A35_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCB7200665.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6227..6352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6228..6275
                     /gene="pheL"
                     /locus_tag="M2A35_004453"
     CDS             6228..6275
                     /gene="pheL"
                     /locus_tag="M2A35_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCB7200666.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6374..7534
                     /gene="pheA"
                     /locus_tag="M2A35_004454"
     CDS             6374..7534
                     /gene="pheA"
                     /locus_tag="M2A35_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCB7200667.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7577..8698)
                     /gene="tyrA"
                     /locus_tag="M2A35_004455"
     CDS             complement(7577..8698)
                     /gene="tyrA"
                     /locus_tag="M2A35_004455"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCB7200668.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8709..9779)
                     /gene="aroF"
                     /locus_tag="M2A35_004456"
     CDS             complement(8709..9779)
                     /gene="aroF"
                     /locus_tag="M2A35_004456"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCB7200669.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9989..10354
                     /locus_tag="M2A35_004457"
     CDS             9989..10354
                     /locus_tag="M2A35_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCB7200670.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10504..11021
                     /locus_tag="M2A35_004458"
                     /pseudo
     CDS             10504..11021
                     /locus_tag="M2A35_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
     gene            11011..>12051
                     /gene="dgcN"
                     /locus_tag="M2A35_004459"
     CDS             11011..>12051
                     /gene="dgcN"
                     /locus_tag="M2A35_004459"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCB7200671.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQI"
BASE COUNT         3104 a         3150 c         2811 g         2986 t
ORIGIN      
        1 atttttgctt tctctgccgg aattctcagg agaaccccgc tgacccggcg gcgtgtttgc
       61 cgttgttccg tgtcagtggt ggcgcattat agggagttat tccggcctga caagcgaaaa
      121 atataaaaac tttatcgttc gctcattttt caggcaaaac atcttaaata tagtcttttc
      181 cgtctaactt atagacaaaa acgagccccg aagggctcgt tttatcattt actggacggc
      241 gacaatccgg tcttcattaa cttccaggcg aatcacttta cccggaacca attcaccaga
      301 cagtatttgc tgtgccagcg ggttttcgat ctgctgctgg atagcacgtt tcaaaggacg
      361 tgcaccatag actggatcgt aaccgttctc gctcagcagt ttcagcgcct cgtcagaaat
      421 gtggatttca taaccacgtt cttccagacg tttgtacaga cgtttcaact gaatctgcgc
      481 aatcgaggca atgtgctgtt cacccagcgg atggaagacc accacttcat caatacggtt
      541 aatgaattcc ggacggaagt tatggcttac cacaccgagc accagctctt tcatgtgcgc
      601 ataatccagt tcaccgaagc gttcctgaat caaatcggaa ccgaggttag aggtcataat
      661 gacaaccgta ttacggaagt cgaccgttct cccctgcccg tcagtcagac gaccatcatc
      721 cagtacctgc aacagaatgt tgaagacatc cggatgcgct ttttccactt catccagcag
      781 gatgacggaa tacggacgac gacgcacagc ttcggtcagg tagccacctt cttcataacc
      841 gacatatccc ggaggcgcac caaccagacg agacaccgag tgtttctcca taaactcgga
      901 catatcgata cggaccatcg cctcgtcgct atcaaacata aagttcgcca gcgccttaca
      961 aagctcagtt ttccccacac ccgttgggcc gaggaacagg aatgaaccaa tcgggcgatt
     1021 tggatccgcc aaccccgcac ggctacggcg aatagcgtta gataccgcat caaccgcttc
     1081 gttctgacca atgacgcgat ggtgcagttc ttgctccata cgcagcagtt tttcgcgctc
     1141 gctttccatc atgcgagaaa ccggaatccc cgtccaacgc gccagcactt cagcaatttc
     1201 ggcgtcggtc actttattac gcaacagacg catagttttg ccttcgagct gcgttgcggc
     1261 ttccagttgc ttttccagtt ccgggatttt gccgtattgc agttcagaca tccgcgccag
     1321 gtcccccaca cggcgagcct gttcaatagc gattttcgcc tgttccagtt ccgctttaat
     1381 ggtctgcgta ccagaaagcg atgccttctc tgctttccac tcttcttcta actcggagta
     1441 ctgacgttct ttgtcgctca gttcttcgtt gagcatatcc aggcgttttt tactggcttc
     1501 atcagactct ttcattaacg cctgttgttc cagtttgagc tggatgatac gacgatcgag
     1561 tcggtcgagt tcttctggtt ttgagtcaat ctgcatacga atgctggatg ctgcttcatc
     1621 gatcaggtcg atggctttat ccggcagctg acggtcagca atgtagcgat gagacaacgt
     1681 cgccgctgca acaattgccg ggtcagtaat ttgcacatgg tggtgcaatt cgtaacgttc
     1741 tttcaggcca cgcagaatcg caatggtatc ttcaacagaa ggctcggcaa caaacacttt
     1801 ctggaaacga cgttccagcg cagcatcttt ttcgatgtac tggcgatatt cgtcaagcgt
     1861 cgtggcacct acgcagtgca attcaccacg cgccagcgcc ggtttcagca tgtttccggc
     1921 gtccattgcg ccatcggctt tacccgcgcc gaccatggta tgtaattcgt cgataaacag
     1981 gatgacgttg ccttcctgtt tggcaagatc gttaagcacg ccttttaaac gttcttcaaa
     2041 ctcaccgcga tatttcgccc cagccaccag cgcgcccata tccagcgcca gtacgcggcg
     2101 gcctttcaat ccttccggca cttcgccgtt gataatacgc tgcgccagac cttcaacgat
     2161 ggcagtttta ccgacgccgg gttcaccaat cagtaccggg ttatttttag tacgacgttg
     2221 cagcacctga atggtacggc gaatttcttc atcacgacca atcaccggat cgagtttgcc
     2281 ctgttcggct cgttcggtaa ggtcgatggt atattttttc aaagcctgac gttggtcttc
     2341 agcaccttga tcgttcacgc tttcacctcc acgcatttgt tcaatcgctt gagtaatgtt
     2401 ggcggtggtc gcccctgctg ctttcaggat gtcggccagc gtgccgcgag actcaagtgc
     2461 cgccagaacg aacagttctg acgagataaa gttatcacca cgtttttgcg ccagcttgtc
     2521 gcaaagatta agaacgcgca ccagatcctg tgatggctgg acatcaccac cagtaccttc
     2581 aacctgcggt aaacgattta atgcctgatt gatatctgtg cgcaactggc cagcatttat
     2641 gccagcggat gttaataaag gactaaccga acccccttcc tgattcagca gggcgctcat
     2701 taaatgaagt ggttcgataa attggttgtc gtgcccgagt gcaagtgatt gggcatcggc
     2761 aagagcaagc tggaatttat tagtaagacg atccagacgc ataactcctc ccataacgga
     2821 tcaaagttgc tactggagat taaatgaggt catccctcaa ttattcaagg ttattgacca
     2881 gattaatgtg aaaagaaaat cacgcgtacc ggatcgtctt gattctttag gttatatcag
     2941 ccaaatgaaa cttgccatac gaccggtggt cttgtcgcga cgataagaga agaaagtctc
     3001 attttccgta tatgtacaac ggtcgccgcc gaaaatttgc tcaacaccca cgttcgccag
     3061 acgctgccgg gcaagctgat aaatatccgc cagatactta tcaccatgct gaatgaaagc
     3121 tgcacttgct ttagcgtcta ctgccataaa cgcctcgcga acctccaccc ccacttcgaa
     3181 cgcgcgtgga ccaattgccg gccctaacca ggcgagaata ttttccggat tatcagcaaa
     3241 acaggaaacc gtctcttcca gcacgcctgc gcacagtcca cgccagccag catgagcggc
     3301 ggcgacttcc gttcccgctc gattgcaaaa cagcacaggg aggcagtcgg cagtcatcac
     3361 tgcgcaaacc gtgccgggcg tattgctata agaggcatct gctcgttttg aggcataagg
     3421 ttcgccagta agcttaagca catctttgcc gtgtacctgc tcaagccaga ccggtttaga
     3481 aggcaaattg cccgcagcaa aaagtcgctt gcgattctcc tcaacgtgat ccgggttatc
     3541 gccacaatgg gcaccgaggt tgagcgagtc atacgggggc aagctcacgc cgccgatacg
     3601 agaagagcta caggccgcaa caccttttgg tagcggccac tgcgggacaa tcagcttact
     3661 cataaccagt ccacttcatc cttatgctct tcgaaatcgg cgcgcatcac ctcaatcagc
     3721 tccaccatat cttgtggaat aggcgcatgc cattccattt cgatgccgga gatcgggtga
     3781 taaagacgca gcatggttgc atgaagcgcc tggcggtcaa acttacgcag catggagata
     3841 aatgcttccg aagcaccttt tggcggacgc ggacggccac cataaaccgg atcgcccacc
     3901 agcggatgag tgatatgggc catatgcacg cggatctggt gcgtacgtcc agtttccaga
     3961 cgcaaccgca gacgcgtgtg cacacggaag tgttccatga tgcgatagtg agtcaccgcc
     4021 ggtttgccca tcggatgcac cgccatgtga gtacgtttgg tcgggtggcg gctgatgggt
     4081 tcatctactg taccgccagc ggtcatatga ccaatcgcca ccgcttcata ctcacgagta
     4141 atttcacgcc gttgcaaaga ttcgactaaa cgcgtctgag ccggaacggt ttttgccaca
     4201 accatcaggc cagtggtgtc tttatccaga cgatggacga tgcccgcacg tggtacatcg
     4261 gcaatgggcg gatagtaatg aagcaacgca ttcagtaccg tgccatccgg gttacccgcg
     4321 ccaggatgta ccaccaggtc gcgcggttta ttaatgacga tgatatcttc atcttcatag
     4381 acgatatcca gcgggatatc ctgcggttca aaacgcgctt cttcttcaat ctcagcgttg
     4441 atggcaacct gctcgccacc caatactttt tctttcggct tatcacaaac tttgccgttt
     4501 accagaacgc gctggtcgag gatccattct tttattcgcg aacgtgaata atccgggaac
     4561 atttcggcca aagcctgatc taagcgttga ccgagttggt tttcggacac cgttgcagtg
     4621 agctgtactc gttgtgccat atatactgct tcttcgttta tcgttggggt tttacggctt
     4681 tgccgtttaa tatagtgtgc tattgtagct ggtcttaacc gggagcagga acagagaatc
     4741 tcccgtatta cattttgagg aaagtcaaaa cgtcatgacg cgcatgaaat atctggtggc
     4801 agccgccaca ctaagcctgt ttttggcggg ttgctcgggg tcaaaggaag aagtacctga
     4861 taatccgcca aatgaaattt acgcgactgc acaacaaaag ctgcaggacg gtaactggag
     4921 acaggcaata acgcaactgg aagcgttaga taatcgctat ccgtttggtc cgtattcgca
     4981 gcaggtgcag ctggatctca tctacgccta ctataaaaac gccgatttgc cgttagcgca
     5041 ggctgccatc gatcgtttta ttcgccttaa cccgacccat ccgaatatcg attatgtcat
     5101 gtacatgcgt ggcctgacca atatggcgct ggatgacagt gcgctgcaag ggttctttgg
     5161 cgttgaccgt agcgatcgcg atcctcaaca tgcacgagct gcgtttagtg acttttccaa
     5221 actggtgcgc ggctatccaa acagtcagta caccaccgat gccaccaaac gtctggtatt
     5281 cctgaaagat cgtctggcga aatatgaata ctccgtggcc gagtactata cagaacgtgg
     5341 cgcatgggtt gccgtcgtta accgcgtaga aggaatgttg cgcgactacc cggataccca
     5401 ggctacgcgt gatgcgctgc cgctgatgga aaatgcatac cgtcagatgc agatgaatgc
     5461 gcaagctgaa aaagtagcga aaatcatcgc cgcaaacagc agcaatacat aacagaaacc
     5521 tgaaatacaa aacggcagcc cttgagctgc cgttttttta ttctgttagt tgtgaaactg
     5581 aagcgattta gtcactatcg atctcatcaa atatggctcg ctttgagata ttcctcaagt
     5641 aaaaaaacac ctcttcctgc gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa
     5701 attatgagat tcccatcaca cattttgaca tcaggagcgg tatgctgaat tcaccaagac
     5761 gggaagacaa gaggtaaaat ttatgacaat gaacattacc agcaaacaaa tggaaattac
     5821 tccggccatc cgccaacatg tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca
     5881 tctgattaat ccacatatca ttctgtccaa agagccacaa gggtttgttg ctgacgccac
     5941 aatcaataca cctaacggcg ttctggttgc cagtggtaaa catgaagata tgtacactgc
     6001 aattaacgaa ttgatcaaca agctggaacg gcagctcaat aaactgcagc acaaaggcga
     6061 agcacgtcgt gccgcaacat cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga
     6121 gtagtccttt atattgagtg tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc
     6181 gtgaaaacag tacgggtact gtactaaagt cacttaagga aacaaacatg aaacacacac
     6241 cgtttttctt cgcattcttt tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa
     6301 cagaatgcga agacgaacaa taaggcctcc caaatcgggg ggcctttttt attgataaca
     6361 aaaaggcaac actatgacat cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc
     6421 gctggatgaa aaattattag cattactggc agagcggcgc gaattggccg tcgaggtagg
     6481 aaaagccaaa ctgctctcgc atcgcccggt acgagatatt gatcgtgaac gcgatttact
     6541 ggaaagatta attacgctcg gtaaagcgca ccatctggac gcccattaca ttactcgcct
     6601 gttccagctc atcattgaag attccgtatt aactcagcag gctttgctcc agcaacatct
     6661 caataaaatt aatccgcact cagcacgcat cgcttttctc ggccccaaag gttcttattc
     6721 ccatcttgcg gcgcgccagt atgctgcccg tcactttgag caattcattg aaagtggctg
     6781 cgccaaattt gccgatattt ttaatcaggt ggaaaccggc caggccgact atgccgtcgt
     6841 accgattgaa aataccagct ccggtgccat aaacgacgtt tacgatctgc tgcaacatac
     6901 cagcttgtcg attgttggcg agatgacgtt aactatcgac cattgtttgt tggtctccgg
     6961 cacgactgat ttatccacca ttaatacggt ctacagccat ccgcagccat tccagcaatg
     7021 cagcaaattc cttaatcgtt atcctcactg gaagattgaa tataccgaaa gtacgtctgc
     7081 ggcaatggaa aaggttgcac aggcaaaatc accgcatgtt gctgcattgg gaagcgaagc
     7141 tggcggcact ttgtacggtt tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa
     7201 cttcacccga tttgtggtgt tggcacgtaa agccattaac gtgtctgacc aggttccggc
     7261 gaaaacgacg ttgttaatgg cgaccgggca acaagccggt gcgctggttg aagcgttgct
     7321 ggtgctgcgc aaccacaatc tgattatgac ccgtctggaa tcacgcccga ttcacggtaa
     7381 tccatgggaa gagatgtttt atctggatat tcaggccaat cttgaatcag cggaaatgca
     7441 aaaagcattg aaagagttag gggaaattac ccgttcaatg aaggtattgg gctgttaccc
     7501 aagtgagaac gtagtgcctg ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc
     7561 atccggcact ggattattac tggcggttgt cattcgcctg acgcaataac acgcggcttt
     7621 cactctgaaa acgctgtgcg taatcgccga accagtgctc caccttgcgg aaactgtcaa
     7681 taaacgcctg cttatcgccc tgctccagca actcaatcgc ctcgccgaaa cgcttatagt
     7741 aacgtttgat taacgccaga ttacgctctg acgacataat aatgtcggca taaagctgcg
     7801 gatcctgagc aaacagtcgc ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga
     7861 gcgccagaag ttgctcaagc tgaacatttt cttctgccag atgcagccca taagcaaaag
     7921 tagcaaagtg gcgcagagcc tgaataaacg ccatattctg atcgtgctcg acagcgctaa
     7981 tacgatgcag ccgagcgccc cagacctgaa tttgctccag aaaccattgg tatgcttccg
     8041 gcttacgtcc atcacaccag accacaactt gctttgccag gctaccgctg tccgggccga
     8101 acatcgggtg taaccccagt accgggccat cgtgcgccgc cagcatggcc tgtaatggtc
     8161 catttttcac tgatgccaga tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc
     8221 cgataacttg ctcagtaacg tggattggca cactaacaat caccattccg gcatcggcaa
     8281 caatatcagc cgctcgatcc cagtcatgtt gctccagaat ccgcacctga taacccgaga
     8341 gggtcagcat cttctcgaac aggcgtccca tctgaccgcc accgccgacg ataaccaccg
     8401 gacgcagtga cggacaaagt gttttaaatc ctttgtcgtt ttcactggag taagattcac
     8461 gcatcacccg acgcaaaaca tcctcaatca gatctggcgg tacacccaga gcttccgcct
     8521 ctgcacgacg cgaggccaac atagatgcct cgcgctccgg aacataaata ggcagtccaa
     8581 agcggctttt cacctcgccc acttcagcaa ccagttccag acgcttagct aataaattca
     8641 gcagcgcttt atcgacttca tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa
     8701 taaacctctt aagccacgcg agccgtcagc agcccgttca gatcctgatg aatttcacgc
     8761 agcaaggcat cggtcatttc ccagctaatg caggcatcgg ttacggatac accgtatttc
     8821 atttcactgc gcggttgctc ggaagactga ttgccctcgt ggatattact ttcgatcatc
     8881 agaccaataa ttgagcgatt gccatctttg atttgagcaa ccacggattc tgccaccgca
     8941 ggctgacggc gataatcttt attggaatta ccgtggctgc aatctaccat cagagacggg
     9001 cgcagtcccg cctgttccat ctctttttca cattgcgcaa catccgcagg gctatagttc
     9061 ggcgctttac caccgcgcag gatgacatgc ccgtccggat tcccctgagt ttgtagcaac
     9121 gcaacctgcc ctgcctggtt aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata
     9181 gcgttaattg ctgttgccag actgccgtcg gtgccgtttt taaaaccaac cggcatggaa
     9241 agcccggagg ccatttcacg gtgagtttgc gattccgttg tacgagcacc aattgctgac
     9301 cagctaaaca gatcgcccag gtattgcggg ctattcggat ctaacgcttc cgtcgccagt
     9361 ggcagtccca tattcaccag ctcaagcagc aatttacgcg cgatctgcag cccggcttct
     9421 acatcaaaag agccatccat atggggatcg ttaattaacc ctttccagcc gacagtggta
     9481 cggggttttt caaaatagac gcgcattacc agatagaggc tatcgctgac ctctgcggca
     9541 agggctttaa atcgacgagc atattccaga gcagtttccg gatcatgaat ggaacaagga
     9601 ccacatacta ccagcagacg aggatcgcgc ccggcgataa tatctgaaat ggttttacgc
     9661 gagtcagcaa tctgggcttc ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc
     9721 ggagtcatta aaacctgttc gtcggtaata tgtacgttat tcagcgcgtc tttttgcatg
     9781 atggcgatcc tgtttatgct cgtttgcgat agttgatcct cagcgaggat gacgtaacga
     9841 taacacataa agtaaagttt tcaatccata tttcgtacat ttttatttac acaggcaatt
     9901 tagtcgcgat ttcacccctt acttctgtat agataaattt acacttcctt tgaaaacaat
     9961 ccgctatgct ttgaaaaagg agaaagaaat gatgaaaaag tttatcgccc ccttgttggc
    10021 tttactggtt agcggatgtc agattgatcc ttatactcac gcgccaacct tgaccagcac
    10081 cgactggtat gatgttggta tggaagatgc gatatcgggc agcgccataa aagatgacga
    10141 tgcatttagc gattcacagg cggatcgcgg tctatacctt aaaggatatg ccgaaggaca
    10201 aaagaaaact tgccagaccg attttactta tgcccgggga ctttccggta aaagctttcc
    10261 tgccagctgc aacaatgttg aaaatgccag ccaactgcat gaagtatggc aaaaaggggc
    10321 tgatgaaaat gccagcacaa tacgattaaa ttaattaaaa ccccacaaat aaattaaaca
    10381 taagatttta ctcattgtgt tttatttctc acattgatga cgatcgcatg aaaaaatgat
    10441 attttaatga gcattctgaa aaaatatcga tgcatttcga gcgaagatgg tggggatccc
    10501 tgaatgcgtt tttctcaccg actgttctta cttctgattc tcctgctgac gggagcacca
    10561 atactcgctc aggagccttc tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt
    10621 gtcagttaca cccgctggcc tgcgctatca gggccaccaa agttatgtat cttttcatct
    10681 tcacgtttta gtaccgccct acaagagaat gccgcgacat ctttgcctta tctgcctgtc
    10741 atcattcaca ctcaacaaga ggcgatgatt tcaggctgca atggttttat tttggcaacg
    10801 agtcaccgac attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa
    10861 ttgccgaaca aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata
    10921 atgacgtcag atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc
    10981 cggatgtctt aatgctcgca cggaagaaaa atgatggata acgataattc tcttaataag
    11041 cgccccacgt ttaaaagagc attacgcaac atcagtatga ccagcatatt tatcactatg
    11101 atgctgatct ggttgctgct ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa
    11161 aaaaacctgg cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt
    11221 gccgatggcc ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg gcaattttca
    11281 actgcagaag tacgtgataa gcagcaaaat attctggcgt cctggcatta cacccgtaag
    11341 gatccaggcg atactttcag caatttcata agccactggc tcttccccgc ccccatcatt
    11401 cagccgattc gtcacaatgg tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt
    11461 tcaatcagcc attttatctg gttttcgctc gccgtactga ccggttgtat tctgctggca
    11521 tcaggcatcg caattaccct cacccgccat ttgcacaatg gcctggtaga agcgctgaaa
    11581 aatatcaccg atgtcgtaca tgatgtgcgt tccaaccgca atttttcccg acgagtttcg
    11641 gaagaacgta tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa
    11701 atggaagagt ggcagcttcg tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta
    11761 catgacccat taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caatacgttg
    11821 atgaacaatt ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc
    11881 aaatatatca atgatacctg gggtcatgcg acgggcgata gagtcttgat tgaaatcgca
    11941 aaacggttag ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa
    12001 ttcgctatgg tgctctatga tgtacagtcg gaatctgaag tgcagcagat a
//
LOCUS       DAHBHC010000116        11792 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHBHC010000116 DAHBHC010000000
VERSION     DAHBHC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.117"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            267..2504
                     /gene="nrfB"
                     /locus_tag="M2A35_004460"
     CDS             267..2504
                     /gene="nrfB"
                     /locus_tag="M2A35_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCB7200672.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2491..5463
                     /gene="nfrA"
                     /locus_tag="M2A35_004461"
     CDS             2491..5463
                     /gene="nfrA"
                     /locus_tag="M2A35_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCB7200673.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     SRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNEFLTIGVHW"
     gene            5464..6354
                     /locus_tag="M2A35_004462"
     CDS             5464..6354
                     /locus_tag="M2A35_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCB7200674.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAGAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6537..7298
                     /gene="envY"
                     /locus_tag="M2A35_004463"
     CDS             6537..7298
                     /gene="envY"
                     /locus_tag="M2A35_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCB7200675.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            7812..8765
                     /gene="ompT"
                     /locus_tag="M2A35_004464"
     CDS             7812..8765
                     /gene="ompT"
                     /locus_tag="M2A35_004464"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HCB7200676.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            complement(9015..9764)
                     /gene="appY"
                     /locus_tag="M2A35_004465"
     CDS             complement(9015..9764)
                     /gene="appY"
                     /locus_tag="M2A35_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AppY"
                     /protein_id="HCB7200677.1"
                     /translation="MDYVCSVVFICQSFDLIINRRVISFKKNSLFIVSDKIRRELPVC
                     PSKLRIVDIDKKTCLSFFIDVNNELPGKFTLDKNGYIAEEEPPLSLVFSLFEGIKIAD
                     SHSLWLKERLCISLLAMFKKRESVNSFILTNINTFTCKITGIISFNIERQWHLKDIAE
                     LIYTSESLIKKRLRDEGTSFTEILRDTRMRYAKKLITSNSYSINVVAQKCGYNSTSYF
                     ICAFKDYYGVTPSHYFEKIIGVTDGINKTID"
     gene            complement(10310..10570)
                     /locus_tag="M2A35_004466"
     CDS             complement(10310..10570)
                     /locus_tag="M2A35_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7200678.1"
                     /translation="MLPQHGDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWKKYRVLLNRVDTSVAPDIEWLIQP"
     gene            10627..11295
                     /locus_tag="M2A35_004467"
     CDS             10627..11295
                     /locus_tag="M2A35_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCB7200679.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGKSKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTGDGTLYGATI
                     LGDGIVHNRFGQKLMRIYNQKGIFSNTRDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(11350..>11792)
                     /locus_tag="M2A35_004468"
     CDS             complement(11350..>11792)
                     /locus_tag="M2A35_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7200680.1"
                     /translation="DIPAGFVAVFNSDDASWHLVEDHRGKTVYDVASGDALFISELGP
                     LPENVTWLSPEGEYQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDA
                     VDLEIATEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
BASE COUNT         3246 a         2869 c         2680 g         2997 t
ORIGIN      
        1 ttgccctgcc tgctcacctc ctccaggcac ttccactaca tgatgacctg atcggtgcca
       61 ccactcctct tcactatgac gttaacctcc cctccttgac accacgcttt tgtctacatt
      121 ctacgcaaac ggcaactttt ataaccagtg taaaaataac gtgccgcaat agtcatacct
      181 attaatggta aaaagctgtc acaattcata aaaaacctta atatacgcca ccctaaacat
      241 aaccagcgtt aatgtaaggt ttttgtgtgg actggcttct tgatgttttt gcaacctggc
      301 tctacggctt aaaagtaatc gcgataacgt tagcggtcat catgttcatc agcggactgg
      361 acgatttttt tattgatgtc gtctactggg tacgccgcat taaacgcaag ttgagtgttt
      421 atcgccgcta cccgcgaatg agttaccgcg aactgtataa accagatgaa aaaccgttag
      481 cgattatggt tccggcgtgg aatgaaacgg gcgtcatcgg caatatggcc gagctggcgg
      541 cgaccacgct cgactacgaa aactatcata tctttgttgg cacctacccc aacgaccccg
      601 atactcaacg tgatgttgac gaagtgtgcg ctcgctttcc gaacgtgcat aaggtagtct
      661 gcgcgcgtcc tggtcccacc agcaaagccg actgtctgaa caacgtgctg gacgccatca
      721 cccagtttga acgtagcgcc aatttcgctt ttgctggttt tattctgcat gacgccgaag
      781 atgtgatttc accgatggaa ttgcgtctgt tcaactatct ggtcgagcgt aaagatctga
      841 ttcagatccc ggtgtatccg ttcgaacgcg aatggacgca cttcaccagc atgacttaca
      901 ttgatgagtt ttcagagctg catggcaaag atgttccggt gcgtgaagcc ctcgccggac
      961 aagtgcccag cgcaggcgtc ggcacctgtt tcagccgccg cgccgtgacc gctctgttag
     1021 ctgacggtga cggtattgct ttcgacgtgc agagtctgac tgaagattac gacattggtt
     1081 tccgcctgaa agagaaaggt atgacggaaa tttttgtccg ttttccggtg gtggacgaag
     1141 ccaaagaacg cgagcagcgt aaatttttac agcacgcacg gacgtcaaac atgatctgcg
     1201 tgcgcgaata tttccccgat accttttcga ctgcagttcg acaaaaatct cgctggatca
     1261 tcggcattgt tttccagggc tttaaaaccc acaaatggac ctccagcctg acgctgaact
     1321 actttctctg gcgcgaccgc aaaggggcaa tcagtaactt tgtcagcttc ctcgcgatgc
     1381 tggtgatgct ccagcttttg ctgttgctgg cgtatgaaag tttgtggccc gatgcctggc
     1441 atttcctttc tatttttagc ggcagcgcat ggttaatgac cctgctgtgg ctaaactttg
     1501 gcttgatggt taaccgcatc gtgcagcggg tgattttcgt cactggctac tacggtctga
     1561 cgcaggggct gttatctgtc ctgcgtcttt tctggggcaa cctgattaac ttcatggcca
     1621 actggcgcgc gctaaaacag gtacttcaac acggcgatcc acgtcgcgtc gcgtgggata
     1681 aaacaacgca tgacttcccc agcgtgactg gcgatacccg ctcgttgcgc ccgttaggcc
     1741 aaattctgct ggaaaatcag gtcatcactg aagaacaact cgatacagca ctgcgtaatc
     1801 gcgtcgaagg tctacgcttg ggcggttcaa tgctgatgca ggggctgatt agcgccgagc
     1861 agctggcaca ggcgctggca gagcaaaacg gcgtggcgtg ggaatccatc gatgcctggc
     1921 agatcccgtc ctcgctgatt gccgaaatgc cggcctccgt agcgctgcat tatgcagtac
     1981 tgccgctgcg tctggaaaat gatgagttaa ttgtcggcag tgaagatggt attgacccgg
     2041 tttcgctggc ggccctgacg cgtaaagtcg gacgcaaagt gcgttacgtc attgttctgc
     2101 ggggacaaat tgtcacgggg ttacgtcact ggtatgcacg ccgacgcggt cacgatccgc
     2161 gggcaatgtt gtacaatgcg gttcagcatc agtggctcac ggaacagcag gccggtgaaa
     2221 tctggcggca atatgtgccg catcagttcc tgttcgccga aatactgacc acgctcggtc
     2281 atattaatcg ttcagcaatt aacgtgttgt tattgcgcca tgaacgcagt tctctgccgc
     2341 tcggcaagtt tttggtcacc gaaggcgtta tcagccagga aacgttggat cgcgtcctga
     2401 caattcaacg cgaattacaa gtttcgatgc aatcactatt actcaaagca ggtttaaaca
     2461 cagaacaggt tgcgcaactg gagtccgaaa atgaaggaga ataaccttaa tcgcgtcatc
     2521 ggatggtctg gtttactgct gacgtcttta ttgagtacca gcgcactcgc agacaatatc
     2581 ggcaccagcg cagaagagct ggggctgagc gattatcgcc attttgttat ttatccccgt
     2641 ctcgataagg cgctgaaggc acagaaaaat aacgacgaag caaccgccat ccgcgaattt
     2701 gaatatatac accagcaggt gccggataat attccgctga ctttatacct tgcggaagcc
     2761 tatcgccatt ttggtcatga tgaccgggcg cggctgttgc ttgaggatca actgaaacgt
     2821 cacccaggag atgcccgact tgagcgcagt ctggcggcta ttccggttga agtgaaaagc
     2881 gttacgactg ttgaagaact gcttgcccag caaaaagcgt gcgacgctgc gccgaccctg
     2941 cgttgtcgca gtgaagtcgg gcagaatgcc ctgcggctgg cacaattacc tgtcgccaga
     3001 gcgcaactga acgatgcgac gtttgctgca tcgccagaag gaaaaacgct gcgaaccgat
     3061 ctgctgcaac gggcaatcta cctgaaacaa tggtcccagg cagatacgct atacaatgaa
     3121 gcacgccagc agaacacatt aagcgcggca gaacgccgtc agtggtttga cgtgcttctt
     3181 gccgggcagc tggacgatcg gatcctggca ctgcaatcac aggggatctt caccgatcct
     3241 cagtcatata ttacttacgc gaccgcgctg gcttatcgtg gcgaaaaagc acgcctccag
     3301 cattatctca atgaaaataa gccgctgttt accacggacg cacaagagaa aagttggctc
     3361 tatctgttat ctaaatacag cgccaacccc gttcaggcat tggcgaatta tacggtgcag
     3421 tttgccgata accgccagta tgttgttggc gcgacgctac cggtgctgtt aaaagaaggt
     3481 cagtacgacg cagcgcaaaa actgctcgcc accctccccg ccaatgaaat gcttgaggag
     3541 cgttatgctg tcagcgtggc gacccgtaac aaggctgaat ctctgcgtct ggcacgattg
     3601 ctgtatcagc aagaaccggc aaatcttacc cgcctggatc aactgacctg gcaactgatg
     3661 cagaacgagc agtcacgcga agctgccgat ttattgctgc aacgctatcc tttccagggc
     3721 gatgcgcgtg tcagccagac tttaatggcg cgactggcgt ctctgctgga aagtcatcct
     3781 tacctggcaa cgccggcgaa ggtggcgatt ttatcgaaac ccttaccgct ggcggagcaa
     3841 cgtcagtggc aaagtcagtt gccgggtatt gcagataatt gcccggcaat agttcgcttg
     3901 ctgggcgata tgtcgccttc ctacgatgcc gccgcctgga accgtctggc aaagtgttat
     3961 cgggacacgc tacccggtgt ggcgctgtat gcatggcttc aggccgaaca acgacaaccg
     4021 aacgcctggc aacatcgtgc ggtagcctat caggcgtatc aggttgagga ctacgccacc
     4081 gcactggcgg cctggcagaa aatcagtctt cacgacatga gcaatgagga tctgcttgct
     4141 gctgccaata ccgcccaggc ggcaggaaat ggtgcagctc gcgatcgctg gctacaacag
     4201 gcagaacaac gtggactggg aaacaatgcc ctctactggt ggctgcatgc gcaacgttac
     4261 attcctggtc agccggaact cgcactgagc gatctcacgc gctcaatcaa tattgcccct
     4321 tctgccaacg cttacgttgc gcgggcgaca atttatcgcc aacgtcataa tgtcccggcg
     4381 gcggtgagtg atttgcgcgc cgcgctggaa ctggaaccga ataatagcaa catccaggca
     4441 gcgcttggtt acgccttgtg ggatagcggt gatatcgcac agtcgcggga aatgctcgaa
     4501 caggcgcata aagggcttcc ggacgatccg gcactgagcc gacaactggc ctacgtgaac
     4561 cagcgtctgg atgacatgcc tgcgacgcag cactacgccc ggctggtgat tgatgacatt
     4621 gataatcagg cgctgataac cccactgacc ccagagcaaa atcagcaacg cttcaatttc
     4681 cgccgtttgc atgaggaggt cggtcgccgc tggacgttca gtttcgattc ttctatcggc
     4741 ttgcgttccg gggcaatgag caccgctaac aataatgtcg gcggcgcagc gccagggaaa
     4801 agctatcgta gctacgggca actggaagcc gagtaccgca tcggacgcaa tatgctgctg
     4861 gaaggcgacc tgctctcagt ttatagccgc gtctttgccg ataccggaga aaacggggtg
     4921 atgatgccgg tgaaaaatcc gatgtccggc accggtctgc gctggaagcc gctgcgcgat
     4981 cagatctttt tcctcgccgt cgaacagcag ttgccgctga acggccaaaa tggcgcatcc
     5041 gataccatgc tgcgcgccag cgcctcattc tttaatggcg gcaaatacag cgacgaatgg
     5101 cacccgaacg gttcaggctg gtttgcccaa aacctgtacc tcgatgcggc gcaatatatc
     5161 cgccaggata ttcaggcgtg gacggcagat tatcgcgtca gctggcatca gaaggtagct
     5221 aacggacaga ctattgagcc ttacgctcac gttcaggaca acggctatcg tgataaaggc
     5281 actcagggcg cgcagcttgg cggagtcggg gtccgctgga atatctggac cggcgagacg
     5341 cactacgacg cctggccgca caaagtcagt ctcggcgtcg agtatcaaca tacctttaag
     5401 gcgattaatc aacgtaacgg agagcgcaac aacgagtttc tcaccattgg agtgcactgg
     5461 taaatgcgta agttcatttt cgtattgctg acactgcttt tggtcagccc tttttccttt
     5521 gcgatgaaag gtattatctg gcaaccacaa aaccgagata gtcaggttac cgatacccag
     5581 tggcaggggc tgatgagtca gttacgtttg caaggcttcg ataccctggt tttgcaatgg
     5641 acccgttacg gcgatgcatt cacccagcca gaacagcgcg cgttattgtt taagcgtgcc
     5701 gcaggtgcgc aacaggctgg cctgaagctg attgtcgggc tgaacgccga tccggaattt
     5761 tttatgcacc agaaacagtc gtccgcagcg ctggaaagct atcttaatcg cctgctggct
     5821 gccgatctcc agcaagccag attatggagc gccgcgcctg gcgtaacgcc ggatggctgg
     5881 tacatcagcg cggaaattga cgacctgaac tggcgcagcg aagctgcccg tcagcctttg
     5941 ctaacatggt taaacaacgc gcagcggctg attagcgatg tttcagcaaa accggtttat
     6001 atcagtagtt ttttcgccgg aaacatgtcg cccgatggct atcgccaact gctggaacaa
     6061 gttaaagcaa ccggcgttaa tgtctgggta caggatggca gcggcgtgga taaacttacc
     6121 gctgaacagc gtgaacgtta tctacaggcc agcgccgatt gccaaagtcc cgcccctgcc
     6181 agcggcattg tttatgaact gtttgtcgcc ggcaaaggca aaacctttac agcgaaaccg
     6241 aaaccggatg cagagatcgc ctcgctgtta gcgaaacgtt cctcttgcgg taaagacact
     6301 ctctatttct ctctgcgcta tttgcccgtc gcgcacggca ttctcgagta ttaaatctcc
     6361 tccaggtaag tcgggtacga cctggcttac ctctttcgct ctcgtcagaa ttccatcaag
     6421 attaattgct aaaaagcgtg ctaaagaaag aagatcgtta tcggcgaacg ggccgatcac
     6481 tcatgcagga agcatagatc ctttgagcgg agtttctgat atctgaggag tgcgaaatgc
     6541 aattgagcag cagtgaacct tgcgtggtga tcctgaccga aaaagaggta gaggtaagcg
     6601 tcaataacca tgctacgttt acccttccga aaaactacct ggccgccttc gcgtgcaaca
     6661 ataacgtcat tgaactctca acgttaaatc acgtattaat cacccacatc aaccgtaaca
     6721 tcatcaacga ttatctgttg tttttaaata agaacttaac ctgtgtaaag ccctggtcgc
     6781 ggctggcaac cccggttatt gcttgtcata gccgtacacc ggaagtgttc cggctagccg
     6841 ccaaccacag caagcagcaa cccagcaaac cctgcgaagc ggagttgacg cgcgtattgc
     6901 tttttaccgt attgtctaac tttcttgagc aatcgcggtt tattgcccta ctgatgtata
     6961 tcttacgcag cagcgtccgc gacagcgtct gccgcattat tcaaagcgat attcagcatt
     7021 actggaatct gcgaattgtc gccagttcgc tatgtttaag ccccagcctg ctcaaaaaga
     7081 aattaaaaaa cgaaaatacc agctatagcc agattgtcac agagtgtcgt atgcgttacg
     7141 ccgtacagat gttattgatg gataacaaaa atatcactca ggtggcgcaa ttatgtggct
     7201 atagcagcac gtcatacttt atctctgttt ttaaggcgtt ttacggcctg acaccgttga
     7261 attatctcgc caaacagcga caaaaagtga tgtggtgaag ggcaaagcgg aaacggataa
     7321 gacgggcata aatgaagaag aaatggcgcg ccctgcagga ttcgaacctg cggcccacga
     7381 cttagaagtt cctagaacga cattttaagt caacaactta ccgcgccatc tctgcgctca
     7441 cacgtcccac tacctcaaaa catgtaaagc cttgcaagcc attgtgaggc cttatgtgtc
     7501 tcagttttgt ccctcttttt tgtactaaaa aacatagtaa ttgaggataa acctcatgct
     7561 attttcgctt atatgcctct aaaggcatgg tacttaaata gataaaagca ccacaaaagc
     7621 ataaaaaaac cacacagtaa aaccgcaata taaaacaata acagataatt aaaccaaaaa
     7681 cagatagcgc attgtgataa tcattcaata ctaaacaaaa tataaacagt ggagcaatat
     7741 gtaattgact cattaagtta gatataaaaa atacatattc aatcattaaa acgattgaat
     7801 ggagaacttt tatgcgggcg aaacttctgg gaatagtcct gacaacccct attgcgatca
     7861 gctcttttgc ttctaccgag actttatcgt ttactcctga caacataaat gcggacatta
     7921 gtcttggaac tctgagcgga aaaacaaaag agcgtgttta tctagccgaa gaaggaggcc
     7981 gaaaagtcag tcaactcgac tggaaattca ataacgctgc aattattaaa ggtgcaatta
     8041 attgggattt gatgccccag atatctatcg gggctgctgg ctggacaact ctcggcagcc
     8101 gaggtggcaa tatggtcgat caggactgga tggattccag taaccccgga acctggacgg
     8161 atgaaagtag acaccctgat acacaactca attatgccaa cgaatttgat ctgaatatca
     8221 aaggctggct actcaacgaa cccaattacc gcctgggact catggccgga tatcaggaaa
     8281 gccgttatag ctttacagcc agaggtggtt cctatatcta cagttctgag gagggattca
     8341 gagatgatat cggctccttc ccgaatggag aaagagcaat cggctacaaa caacgtttta
     8401 aaatgcccta cattggcttg actggaagtt atcgttatga agattttgaa ctcggtggca
     8461 catttaaata cagcggctgg gtggaatcat ctgataacga tgaacactat gacccgggaa
     8521 aaagaatcac ttatcgcagt aaggtcaaag accaaaatta ctattctgtt gcagtcaatg
     8581 caggttatta cgtcacacct aacgcaaaag tttatgttga aggcgcatgg aatcgggtta
     8641 cgaataaaaa aggtaatact tcactttatg atcacaataa taacacttca gactacagca
     8701 aaaatggagc aggtatagaa aactataact tcatcactac tgctggactt aagtacacat
     8761 tttaagaacg ccaactaaaa tttccccgag gtgaaaatcg ccccggggaa taactagcca
     8821 tttcaatgta acaattaacc cttaaaataa acccagaagg ttattaacta aatcacatag
     8881 aaaaccatca attatagtat gtataaaata ggcgacagca acccaattac aaattaatgg
     8941 ttccagaata tcacatcaaa aaacacgctg tataatatta taattaacat gtagacaact
     9001 tgtaataaac attatcaatc aattgttttg tttattccat ctgtgacgcc gattattttc
     9061 tcaaaataat gagatggcgt gacaccataa taatctttaa atgcacatat gaaatatgaa
     9121 gtactgttat agccacattt ctgggctacg acattgatag aataagagtt tgaagttatg
     9181 agtttttttg catacctcat cctagtatct ctcaatattt cagtaaatga cgttccttca
     9241 tcccttaatc ttttttttat taaactttca ctcgtataaa tcaattccgc aatatctttt
     9301 aaatgccatt gccgctcaat attaaaactg attattccag taattttaca ggtaaatgta
     9361 tttatatttg ttagtataaa tgaatttaca ctttcgcgtt ttttgaacat ggcaagtaag
     9421 gatatacata gtctttcttt taaccaaagg gagtgtgagt ctgctatttt aatcccttca
     9481 aacagagaaa aaacaagcga taatggaggt tcctcttcag caatatagcc attcttatca
     9541 agagtaaatt tgccaggcag ctcattattc acgtcgataa aaaaggataa acatgttttc
     9601 ttatctatat caacaattct tagtttagag gggcatactg gtaactccct tctaattttg
     9661 tcgcttacaa taaacaatga gttttttttg aacgagataa ctctcctgtt tataattaaa
     9721 tcaaatgatt gacagatgaa aactacggag caaacataat ccatcttgca cctatcataa
     9781 aattaaaaca agttgatagt agtcaaataa caaccaatta aatacacaat cataatcagg
     9841 atgatgtgca tttatatttt tatacacaaa attatagttt gcaaatttta ataaatttca
     9901 tttaagatta aattattata tgtatattgt tttttattct aacgtatttc aaagttacat
     9961 ttttcaacgc ttactatgct ttttattaac ataaactcac tacaacgcac ctgaaacctc
    10021 ttgctatata tatgtcaacc gtttgaattt aaaataaaaa gagtatcatt tttacttgca
    10081 tttcttatca agtcacattc aacaacagta aaaaaacatt attagaacca ttcaattaac
    10141 aaaaaaccaa catccagctt gcttaatttt tctttattaa acgatattga aaatcaattg
    10201 ataaaataca tctaaacaac cttttggggc gcaaaagcat aacatcaaac aaacaaataa
    10261 cacaccgaaa aaactcacaa ttaataacct atgatataca tactgtttat tatggttgaa
    10321 taagccactc gatatctggt gctacggaag tgtccacacg gtttagcagc acccgatact
    10381 ttttccaggc ttccagcaac gatctttctt cctccgttgc gatttccaga tctacagcat
    10441 cctgcagtgg cgcaatatac tcactgaatt cctggatgta gaactgtgtg gtgacggtct
    10501 tccagccatt cggctcctgc tgtattgaag cataccaggc tatttcaata tcgccatgct
    10561 gcggcagcat ttaacccctt gtaattcatt gccataattg atttaattca caaataaaac
    10621 tataacatgg tgaaattaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
    10681 cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga
    10741 tgccctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
    10801 gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
    10861 ttaatggatt tgaatgaagc tagcctgaac gcggcatcta caagggctgg gaaatcaaaa
    10921 attaaacata aaattagcca tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa
    10981 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
    11041 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactggcg atggaactct atacggagcc
    11101 acaattcttg gcgatggaat tgtgcacaat cgcttcggtc aaaaactgat gcgcatttac
    11161 aatcagaaag gcatcttttc aaacacaaga gattccgaag aaggcttaac acatatactc
    11221 tcagagcatt tcgagaatgt taaaaccaag gttcaaggta ctgtagtaat gttttccgct
    11281 tcagggaaaa aatagcatcc aaccgcagca cgttcttgca tacgacgtgc tgcggcataa
    11341 tcccaatgat tactccctga cagggttcgt aggccactca atatcaggtg cagttgatgt
    11401 atcaacacgg ttcagcaaca cccgatactt tttccaggct tccagcaatg aggtttcttc
    11461 ctccgttgcg atttccagat ctacagcatc ctgaagtggc gcaatatgct cactggctac
    11521 ctgcatcagg ctgttttttg tttcttccgc ctcccggatc cggaatagtt tttctgcttc
    11581 tgcatctttc acccaggctg tgccgttcca cttctgatat tccccttccg gcgataacca
    11641 ggtgacattt tccggtaacg gaccgagttc agaaataaat aacgcgtccc ctgatgccac
    11701 gtcataaact gttttacccc gatggtcttc aacaagatgc cacgatgcat catcactgtt
    11761 gaaaacagcc acgaagccag ccggaatatc tg
//
LOCUS       DAHBHC010000117        11735 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHBHC010000117 DAHBHC010000000
VERSION     DAHBHC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.118"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            305..1603
                     /gene="kgtP"
                     /locus_tag="M2A35_004469"
     CDS             305..1603
                     /gene="kgtP"
                     /locus_tag="M2A35_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCB7200681.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVDGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1600..1923)
                     /locus_tag="M2A35_004470"
     CDS             complement(1600..1923)
                     /locus_tag="M2A35_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCB7200682.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1969..3324)
                     /gene="pssA"
                     /locus_tag="M2A35_004471"
     CDS             complement(1969..3324)
                     /gene="pssA"
                     /locus_tag="M2A35_004471"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCB7200683.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3438..6098)
                     /gene="pat"
                     /locus_tag="M2A35_004472"
     CDS             complement(3438..6098)
                     /gene="pat"
                     /locus_tag="M2A35_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCB7200684.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6130..6828)
                     /gene="tapT"
                     /locus_tag="M2A35_004473"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6130..6828)
                     /gene="tapT"
                     /locus_tag="M2A35_004473"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCB7200685.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6897..7316)
                     /gene="trxC"
                     /locus_tag="M2A35_004474"
     CDS             complement(6897..7316)
                     /gene="trxC"
                     /locus_tag="M2A35_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCB7200686.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7523..8560
                     /locus_tag="M2A35_004475"
     CDS             7523..8560
                     /locus_tag="M2A35_004475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCB7200687.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8608..9297)
                     /gene="ung"
                     /locus_tag="M2A35_004476"
     CDS             complement(8608..9297)
                     /gene="ung"
                     /locus_tag="M2A35_004476"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCB7200688.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9602..9985
                     /gene="grcA"
                     /locus_tag="M2A35_004477"
     CDS             9602..9985
                     /gene="grcA"
                     /locus_tag="M2A35_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCB7200689.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10041..10628)
                     /gene="eamB"
                     /locus_tag="M2A35_004478"
     CDS             complement(10041..10628)
                     /gene="eamB"
                     /locus_tag="M2A35_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCB7200690.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10731..11612
                     /locus_tag="M2A35_004479"
     CDS             10731..11612
                     /locus_tag="M2A35_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCB7200691.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2917 a         2930 c         3034 g         2854 t
ORIGIN      
        1 tcagtcacga tggccttttt gcgttggcgc agaaaaaaat gcctgatgcg acgctgcgcg
       61 tcttatactc ccacatatgc cagattcagc aacgaatacg gcttccccaa cttgctcact
      121 tccatacgtg tcctccttac cagaaattta tccttaagct cctcaataat cattttcctg
      181 ctaactaaat tcatggttaa ggttgcataa tgatatgcaa caaatgtata acattttatt
      241 taccaaaaga aataaacaaa agtgaccgac aaaagcatcg gattacggca ggagacataa
      301 tggcatggct gaaagtactg taacggcaga cagcaaactg acaagtagtg atactcgtcg
      361 ccgcatttgg gcgattgtgg gggcctcttc aggtaatctg gtcgagtggt tcgatttcta
      421 tgtctactcg ttctgttcac tctacttcgc ccacattttc ttcccttccg ggaacacgac
      481 gactcaacta ctacaaacag caggtgtttt tgctgcggga ttcctgatgc gcccaatagg
      541 cggttggcta tttggccgca tagccgataa acatggtcgc aaaaaatcga tgctgttatc
      601 ggtgtgtatg atgtgtttcg gatcgctggt tatcgcctgc ctcccaggtt atgaaactat
      661 aggtacgtgg gctccggcat tattgcttct cgctcgttta tttcagggat tatccgttga
      721 cggagaatat ggcaccagcg ccacctatat gagtgaagtt gccgttgaag ggcgcaaagg
      781 tttttacgca tcatttcagt atgtgacgtt gatcggcgga caactgctag ccctactggt
      841 tgtcgtggtt ttacaacaca ccatggaaga cgctgcactc agagagtggg gatggcgtat
      901 tcctttcgcg ttaggagctg tgttagctgt tgtggcgttg tggttacgtc gtcagttaga
      961 tgaaacttcg caacaagaaa cgcgcgcttt aaaagaagct ggatctctga aaggattatg
     1021 gcgcaatcgc cgtgcattca tcatggttct cggttttacc gctgcgggct ccctttgttt
     1081 ctataccttc accacttata tgcagaagta tctggtaaat actgcgggaa tgcatgccaa
     1141 cgtggcgagt ggcattatga ctgccgcatt gtttgtattc atgcttattc aaccactcat
     1201 tggcgcgctg tcggataaga ttggtcgccg tacctcaatg ttatgtttcg gttcgctggc
     1261 agccattttt accgttccta ttctctcagc attgcaaaac gtttcctcgc cttatgccgc
     1321 ttttggtctg gtgatgtgtg ccctgctgat agtgagtttt tatacatcaa tcagtggaat
     1381 actgaaggct gagatgttcc cggcacaggt tcgcgcatta ggcgttggtc tgtcatatgc
     1441 ggtcgctaat gctatatttg gtggttcggc ggagtacgta gcgttgtcgc tgaaatcaat
     1501 aggaatggaa acagccttct tctggtatgt gaccttgatg gccgtggtgg cgtttctggt
     1561 ttctttgatg ctacatcgca aagggaaggg gatgcgtctt tagtgacggg tcagttgcca
     1621 gacggtatag ccggtgcttg caccggcgac atcccaggcc aaatccttcc agctccagcc
     1681 gctcccttcg gggcggctat cccaaagctc ttttgacgcc cccaaactga cagagaacat
     1741 caatccaaac atggcactgc gatcccggct catcccctga tgctgtgaat attcatttcc
     1801 ggcggcggaa agcatcgccg aggcgataaa gtgttgagct ttatcctgcc cgctccagct
     1861 atcgttagcc atatggctgc atccagaaag taacaatagc gaacagacaa aaagaatacg
     1921 caaaacgtgg cctccaaaaa acaaaccccg tacaggacgg ggttgtgatt acaggatgcg
     1981 gctaattaat cggtcgatgc ggatacggcg caaacggcgg atgagtttac gaaccttcac
     2041 cggataatcg gcaatacttt gcagatcgcg atagtgctta acgatggtgg tatgctcgcg
     2101 gatcagctcc agttctttct ctcgctgcgg cgcaagctcc agttgtggat cgtggatcaa
     2161 aatggcgttt tccagatcca gacgccaggc gcgcgggttc aggttattac cggtgatcaa
     2221 catccactta tcatcaaccc acatcccttt taggtgatag gtgttgtcgt catctttcca
     2281 taaccgaacc actagctggt cagtattgac gtaatactgc aaacggctca ggaaacgacg
     2341 cagattgatc tcatagagat aaggcaatgc gccaattatc ttgaaaggtt catcttccgg
     2401 aatgtagaag tcgttcgcgg ttttatcacc aacaataatt tcgacctttt tcccttcgcg
     2461 cagcaactgg ataatattgc gcacaaggat tgctggcagg ttgaagtatg gcgtacagat
     2521 ggttagtttc tgctcggcac aaggcataag atggaaaatg gtcttgttca acagactcga
     2581 tttccccagc cccactagcg gcgttacgga aagctgatcg ttgtcggcat cgccctggaa
     2641 atgataagcg gcatcacgca gctcctggcg gaacagacga atatcgttct tgatttccgg
     2701 gctttttggc cgattaacat catccagacg attaacgccg cggccattca taatattctg
     2761 tgtaacccat tcaaacataa tgtctgacat cttacggtta cggatcagat gataacggtc
     2821 gtagcgatat ttatcgtgct gatgcaggta aacatcgttc aggctggcac cgctataaag
     2881 tacgctatcg tcgatgataa agcctttaaa gtgcagaaca ccaagggctt cacgggtatt
     2941 gattggaacg ccataaaccg gaacatctac gcccggattt tcctgcgcca tgcggcagta
     3001 ccagtcagcg ttagtgttag atgccgcagc gccaatgcgt ccacgttgtg cacgatgcca
     3061 gtcgaccagc acccgcacat ccagttccgg acgctgcctt ttagcttcat acaacgcgtt
     3121 cagaatgcct ttgccaccgt catcctgttc gagatacagg gcgacaatgc aaatgcgctg
     3181 cttcgcgctg gctatttttt ccagcagcgt ctcccggaag tcggcgggag cgtaaaagaa
     3241 atcgacatca tcaactgatt gagaaatctt gggtagttgg gcaaggtgtt gttgatgttt
     3301 attacgctta aattttgaca acatcacagt gcatttcttc tctgttcatt gaagggtcct
     3361 ctgtgcaatg cagacgacat aagcgggcaa taataacacc agtcccgatt aagtggtcaa
     3421 catttccagt accttactca tgattcctcg cgttgggcaa gatttagcgt aagcccaacg
     3481 atcccctctt cgagctggat atcaacgtta aacccgagct tgcgggctag cgccaccatg
     3541 ccacgattgt ttggcatcgt aataccattc agacgttgta gtccgtgatc tcgcgtatag
     3601 gtaatcaact tttccattaa gcgtcgacct aagcctaacc ctttgagatc cgagcgaacc
     3661 agcacagcaa attcggcatc gatgttatca ggatcggaaa tcgcacgcgt gacgccgagg
     3721 atctcttccg tttgatcaat acgtcgtacc gctacaaacg ccatttcccg atcgtagtcg
     3781 atctgtgtca tgttggctaa atcttcatgg gtaaattcgt tgatctcgct aaagtagcgg
     3841 taataaagat cttctttggt gactcgcgaa atgaattgct gaagttgtgg ctcatcttct
     3901 ggcaaaatcg ggcggaacaa gcagcgttca ccgtttttca attctaccca ttcttccagc
     3961 tgatgcggat aagggcgcac tgccagccga ctctcgttat cgccttcaaa cggcgagata
     4021 tccagcgtga catccagcgc ggtaaattca ctgccagaag ccagcaaagg atgaatatcc
     4081 agacgctgaa tttccgggca atcgacaatc aagttggaaa cctgcaccag aagctggctc
     4141 aagcctgcaa catccaatgg gcgtagcgca ctgcgcgcac gaatcttttt acttttgatc
     4201 ccctgaataa ccagatagcg ggccaggttc atgttcagcg gcggcagtgc gacgacggct
     4261 tgatcttcag gacgccactc cacaccgcct tcacccagca tgatcaacgg cccgaaaacc
     4321 ggatcgtgct caaccacaac ccgcaactcc tgagcgccag cacggttagc catactttgc
     4381 accaacaggc cgtggacccg cgcctgtggc caggccattt ttacgcgatc gaaaatagcg
     4441 ttcgccgctt gctggacttc attggctgta cgcaggtaaa gcatgacgcc ctgaacttcc
     4501 gatttatgtg gaatatccgg cgaacgcaat ttcagcgcca ccggataacc aatctgttcg
     4561 gcaatatgca ccgcttcggt gctatcgctg gcaatccagg tagggagcgt gttcatgcca
     4621 tacgcttgca ggatgggctg aacttcatgg gtatcgagcg acgtagcccc ttcggcaatc
     4681 gcctgttgca acagaagatg cgcttctgcg gtattggaag tcagattgct gggcaacgcc
     4741 ggcgtttcgc gtagttgctt ctgattacgc cggtactcca ccatatgcat aaaagcagtg
     4801 atggttcctt ccggggtacg gtaggtcggc agcccggctt cgctgaataa tcgtcgtgcc
     4861 tcttgcgagg aatgttcgcc acaccagttc gtcagcagag aaacatactt gctgcgggga
     4921 tgatgcttta ccgcttcaat taatacttgc gcgctttctg ttgcgggagc agcggcgctg
     4981 ggcgaatgaa taaccatcag cgcatcaaaa tcctggctgt ggagcagaat gtccagcgtt
     5041 ttaatatagt gctcactgct ggcgtcatcg cgcagatcga gcgggttaga gattgccaca
     5101 tgtcctggca gtgcatcgcg cagtttctgg caggtttctt cgcttagcgt tgccagcttg
     5161 ccattgcgtg accataaggc atccagcgcc agcgcggcag gcgcagcacc gttgctgata
     5221 atcatcaacc ggtcgccacg cagcgggcgc atatggctaa gggtttccac cgccgaaaac
     5281 agttcgtggg tatcctgtac ccgcaacaaa ccggcacgct gaatagccgc atcccatgcc
     5341 gggtccattc ctgccgtcgt gttgagcagt cgctgtgccg ccgggctacg tccgctttta
     5401 atcaccagaa tcggtttatt acgcgaggca ctacgggctg ccgaaacaaa gcgtcgcgcg
     5461 tcgcttaact gttcgagata gagcaggatg gcgctggttt tactgtcgcg cgccagatag
     5521 tcgagcaatt catcaacgtc gatatccagg ctgtcgccga gcgcaataaa gtaggaaaag
     5581 cccatctcac gctgttgcgc ccagtcgagg atggtgttgg agacggctgc cgattgtgaa
     5641 ataaacgcca gcttgccgcg tttaatcggc acaggcgaaa agctggcatt cagaccttgc
     5701 cagggagcca gtaaacccag actgtttggt ccaagcaggc gcatgttatg gcgcagggcg
     5761 caggcgcgga gatcttcgtg ttgcgatgcc ggggcggaaa gaataatgca ggttttacag
     5821 cctttctcgc cgagctcttc cagaagagca agattacggc tggcattggt acataaaacc
     5881 gcaaggtcgg gtgtaaaggg caagctggca atatccggcc aggccaacac acccaacacc
     5941 gctttccagg ctggcgtcac cgggagtacc ggtccgttaa agcctcccgc cagcaggtta
     6001 cgcatcatca ggtaacctgc gcgattgggt ttcatcgacg cgccaattac cgctatcgat
     6061 tttggtcgca gtagtgcttc cagtcctcgc tgactcatac cggtctcccg cagaagtgac
     6121 cgaatgattt taaacgcttt ctaactgttc tgctgtgatg ctacccagat gttgcgtttt
     6181 tcctgccaga tagcgtgttt taaagcgggt aaaatgctcg cctaaccctg ctgccgcccc
     6241 ggtatcgctg gccatatcta acagtgcgat ggctacctcg gcagtacaat attggcctgc
     6301 agcctgggct tcacgcaggc gataggcaga aagccgggaa agatcgacgg aaatgacggg
     6361 aagattatcc agatacggac ttttacgaaa catcttgcga gcttccggcc aggtaccatc
     6421 gagcatgata aacagcggtg gcttaccggc aggtggtgtg aagatcactt cccgttgctc
     6481 atcagcatac gaagcgggaa agaccaccat tggctgatag tccgggtttt gcaccaactc
     6541 cagcaaatcc tgcgagggtt cggtacgcga ccattgaaac gcaacggtat caggcaaaat
     6601 atcagcaatc agacgcccgg tattactggg cttcattggc tcggtgtcga acatcagcaa
     6661 acagaagcga ctttttgctt gtgctggggt aattgtcgaa cagagacata atttctctgg
     6721 caaaagacag cgttggcagc gacgaacgcg attaccgcgg gcaagaaaag gacgtgttgc
     6781 gcgcgcaata cgctcggcgc gtaactggag aacagcgttt tcggtcataa gagagcgtcg
     6841 aaaaaacgcc attgtcgcag aggagaaaac ggggcacaag atgcgccccg gtaagattaa
     6901 agagattcgt tcagccagct atcgaacggc gctttcggta ctgcgccatt aagcatgtcg
     6961 acaacctgac cgtttttgaa aatcatgatc gtcggtatac tacgaattcc aaagcgactg
     7021 ctcaattcac gttcagcttc ggtattcact ttcacaaagc gcactttacc gctacgctct
     7081 tgcgcgacat cttcaaaaat tggtgcgaaa ttacggcagg ggccgcacca cggtgcccag
     7141 aagtcgatca ccacaggtag atcatccttc agcaatttgt cgagtgtttc accggtcgca
     7201 ttaatcacct ctccgtcaaa caagtcgtga ccgcagcgtc cgcattttgc cgcatcttcg
     7261 atccgatcgt cgggaatgcg attgatggcc tgacaatggg tacaaacggt attcataact
     7321 aacctcggga tgagtaagga gacttatcgg caacgacgcc ggtatgtttc taatatgtta
     7381 catattatcg gggacattgt tccagacaac aatctgtttt attcaatagg tatgatagtc
     7441 gcaggctttg gtacgaaatc atggcgaacc gcgcggactt cgggtaatct gcgcgcttcg
     7501 cgcagcgctg gtggagaaaa gcatgaacga tgaaatgaaa ggtaaaagcg gcaaggtcaa
     7561 agtgatgtat gtccgcagtg atgatgattc tgataaacgt acccacaacc cgcgtaccgg
     7621 gaaagggggc gggcgtccag gaaaatctcg tgctgacggt ggccgtcgcc ccgcccgcga
     7681 tgacaaacag agtcagcccc gtgaccgcaa gtgggaagat tcgccgtggc gcacggtttc
     7741 ccgcgcgccg ggtgatgaga cgccggaaaa ggccgatcac ggtggcatca gtggtaaaag
     7801 ttttattgat ccggaagtgt tgcgtcgtca gcgtgcggaa gaaacccgcg tctacggcga
     7861 aaatgcatgt caggcacttt tccagagccg tccggaagcg attgttcgcg cctggtttat
     7921 ccagagtgta acgccgcgtt ttaaagaagc cttgcgctgg atggcagcaa accgcaaagc
     7981 gtaccatgtg gtggatgaag cggaattgac aaaagcgtca ggcacggaac atcacggcgg
     8041 cgtttgcttc ctgatcaaaa agcgtaatgg cacaaccgtg cagcagtggg taagccaggc
     8101 aggcgcgcag gattgtgtac tggcgctgga aaacgaatct aacccgcata acctgggtgg
     8161 catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt gtggtgcagg atgcggcact
     8221 gctggagtcg ggggcggcta tccgtaccgc agaaggcggc gcagagcacg ttcagccgat
     8281 tactggcgac aacattgtta acgtgttgga tgatttccgt caggcaggtt acaccgtagt
     8341 gaccacttcc agcgagcagg ggaaaccgct gttcaaaacc agtctgccag cgaaaatggt
     8401 actggtgctg ggtcaggaat atgaagggtt accggatgcc gcacgcgatc cgaacgatct
     8461 gcgcgtgaag attgacggta cgggcaacgt tgccgggctg aatatctctg ttgcaaccgg
     8521 cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga taaatcagcc gggtggcaac
     8581 tctgccatcc ggcattttac cgcaaattta ctcactctct gccggtaata ctggcatcca
     8641 gtcaatcggc gtctcgccac gttgttccag ccactgattt gccagcacaa aatggttgca
     8701 gccaaagaat ccacgatgcg ccgaaagcgg cgacggatgc ggtgctttca gtacatgatg
     8761 gcgttgctta tctataatcg cccctttctt ttgcgcatgt gatccccaca acaaaaacac
     8821 cacgccttcg cggtgctggt taatcaggct gatgacttta tcagtaaacg tctcccaacc
     8881 gaggctggcg tgggaatgcg cctgacctgc gcgaaccgtc aacacagtat tgagcagcag
     8941 aacgccctga cgcgcccagc tttcgagata gccatgatta gggcgggtga agcccggaat
     9001 agtattttcc agctctttat acatattcaa taatgacgga ggaatggcaa tgccgggacg
     9061 aacggaaaat gccagaccat gcgcctgtcc cggtccgtga taaggatcct ggccgagaat
     9121 caccacttta acgtcaccca actcggtaaa gcggaacgcg ttaaagacat ctttttgtgg
     9181 tggatagata gtgacgccgg actgccgctc gctggcgacg gtctgaaggg tattaagaaa
     9241 ataaggttgt tgcttctctt cagccagcac gtcatgccag gttaattcgt tagccatctc
     9301 gctctcctgc gaatcttcaa tccgcctagc ttaactgctt ctttctacag aacaaaatca
     9361 tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa attggtaaaa tttcacaccc
     9421 gcataagttg atgtaaaaca ataaaatcca tttacgacca caatcgatgt gtggttttta
     9481 ttgatttaaa tcaaagaatc aagggtggtt gaggagtata tatacactca agcaacaatg
     9541 gttttaccaa ttggccgcga caggctgaac aaatcaaata attttgccgg ggaggcatca
     9601 catgattaca ggtatccaga ttactaaagc cgctaacgac gatctgctga actctttctg
     9661 gctgctggac agcgaaaaag gcgaagcacg ttgcatcgtt gcaaaagcag gttatgcaga
     9721 agatgaagtg gttgcagtaa gcaaactggg tgacattgaa taccgtgaag ttccagtaga
     9781 agtgaaacca gaagttcgcg ttgaaggtgg tcaacacctg aacgttaacg ttctgcgtcg
     9841 tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat ccgcagctga ccatccgtgt
     9901 atccggttat gcagttcgct ttaactctct gactccggaa cagcagcgcg acgttatcgc
     9961 tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag ttactaagcg gaagagggcg
    10021 acctcttccg ctttttttcg ttaatagaaa atgcgtaccg cgcaatagac cagcaacagg
    10081 gcaagcacga tatttaactg gcgaccatac tggcgaaaca atcgctgaaa cagatgcccc
    10141 gccagcgccc agcacacatt gccaaacgtc ccaatcatcg ccagcaaaac gctgacgcca
    10201 actacccagc ttagcgcctg tgtttgcggc agaacaaacg tcgacagtgc cgtaacaccg
    10261 tacaaaatga ttttgacgtt cacaaactgc aaagcaaagc tggcccaaaa gctgattggt
    10321 tttgcctgaa gtccgtcttc ctttgttggg ctggtggcga ttttccacgc cagccagaca
    10381 atatatgccg cccccgccca actcaaaagg tgtaccgctg ccgggtcaat cactgccagt
    10441 gaaaatgaaa tgcccgcaca cagtaacatc acaatcaaaa atcccagact catccctgcc
    10501 agcacgcggg tactttgacg aaatccatgc gacgtagcag agctaagggc gagaatattg
    10561 ttcggtcctg gcgtcatagc ggtaatcagg gtgtaagtcc aaaaagcact taaaagggtc
    10621 ggtgtcactt gttatctcct ctgttttggt agacagtacc cgacacggaa catttaaaaa
    10681 aatgaatgtt tttatattct agattcgata ttttcgatag gtttggggtt atggatctgc
    10741 gccgttttat tacgcttaaa accgtggtgg aagagggttc ctttttgcga gcttcgcaaa
    10801 aattgtgctg tacacaatcg acggtgactt ttcatattca gcagcttgag caggagtttt
    10861 cagtccagtt atttgagaaa attggccgac ggatgtgcct tacccgcgaa ggaaaaaagt
    10921 tgctgccgca catttatgaa cttacccgag tgatggatac gcttcgtgag gctgccaaaa
    10981 aggagtcaga tccggacggg gaactacgcg ttgtttcggg cgaaacgttg ctctcttacc
    11041 gaatgccgca ggtgttgcag cgttttcggc aacgcgcacc aaaagtgcgt ttatcgttgc
    11101 agtcgctgaa ctgttacgtg atccgtgatg cgctactgaa tgatgaggcc gatgtcggcg
    11161 tcttttatcg cgtagggaat gatgatgccc tgaaccgacg agagttgggt gaacaatcgc
    11221 tggtgctggt ggcttcaccg caaattgcag atgtcgattt tactgagccg ggaagacata
    11281 acgcctgtag ctttattatc aacgaaccgc aatgtgtctt ccggcagata tttgagagca
    11341 cgctgcgtca gcggcggatc acggtggaaa acaccattga gctgttaagc attgaaagta
    11401 tcaaacgttg tgtcgcggcg aatatcggtg tcagttactt gccccgcttt gcggtggaaa
    11461 aagagcttga gagcggagaa ttaattgaat tgccttttgg cgaacagtcg cagaccatta
    11521 cggccatgtg cgcacaccat gctggaaaag cggttagccc ggcgatgcac acttttattc
    11581 agtgtgttga agagagtttt gtggctggat aaaaataatg tcgggattaa acccggcatt
    11641 taacttgtcg acaaagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatccggc
    11701 ctacaagatc gtgcaaattc aatatattgc atgag
//
LOCUS       DAHBHC010000118        11552 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHBHC010000118 DAHBHC010000000
VERSION     DAHBHC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.119"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..260)
                     /gene="tccP2"
                     /locus_tag="M2A35_004480"
     CDS             complement(<1..260)
                     /gene="tccP2"
                     /locus_tag="M2A35_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HCB7200692.1"
                     /translation="MINSINSFFSGLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFS
                     PSNSPASATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPL"
     gene            complement(863..950)
                     /locus_tag="M2A35_004481"
     tRNA            complement(863..950)
                     /locus_tag="M2A35_004481"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Ser,seq:tga)
     gene            1377..2495
                     /gene="hyaA"
                     /locus_tag="M2A35_004482"
     CDS             1377..2495
                     /gene="hyaA"
                     /locus_tag="M2A35_004482"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCB7200693.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2492..4285
                     /gene="hyaB"
                     /locus_tag="M2A35_004483"
     CDS             2492..4285
                     /gene="hyaB"
                     /locus_tag="M2A35_004483"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCB7200694.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRNAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4304..5011
                     /gene="hyaC"
                     /locus_tag="M2A35_004484"
     CDS             4304..5011
                     /gene="hyaC"
                     /locus_tag="M2A35_004484"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCB7200695.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLLLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5008..5595
                     /locus_tag="M2A35_004485"
     CDS             5008..5595
                     /locus_tag="M2A35_004485"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCB7200696.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5592..5990
                     /gene="hyaE"
                     /locus_tag="M2A35_004486"
     CDS             5592..5990
                     /gene="hyaE"
                     /locus_tag="M2A35_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCB7200697.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMISELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5987..6844
                     /locus_tag="M2A35_004487"
     CDS             5987..6844
                     /locus_tag="M2A35_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCB7200698.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQV
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6978..8522
                     /gene="appC"
                     /locus_tag="M2A35_004488"
     CDS             6978..8522
                     /gene="appC"
                     /locus_tag="M2A35_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCB7200699.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8534..9670
                     /gene="appB"
                     /locus_tag="M2A35_004489"
     CDS             8534..9670
                     /gene="appB"
                     /locus_tag="M2A35_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCB7200700.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNND
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9683..9775
                     /gene="cbdX"
                     /locus_tag="M2A35_004490"
     CDS             9683..9775
                     /gene="cbdX"
                     /locus_tag="M2A35_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCB7200701.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9851..11149
                     /gene="appA"
                     /locus_tag="M2A35_004491"
     CDS             9851..11149
                     /gene="appA"
                     /locus_tag="M2A35_004491"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCB7200702.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSESELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSKLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11269..>11552)
                     /locus_tag="M2A35_004492"
     CDS             complement(11269..>11552)
                     /locus_tag="M2A35_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCB7200703.1"
                     /translation="LDWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNT
                     AKEVSLSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT         2628 a         2921 c         3205 g         2798 t
ORIGIN      
        1 agaggccgat gtaaagcgtt tttttctata cttgactgag tataagatgt gggacgttga
       61 agtcctgatt ctgtataaga attttttacc ttaaaaatag ttgttgcact ggcaggactg
      121 ttgctgggag aaaaaggtga gctggttttt accgtgttag gtgtgctttt gtgctgcgag
      181 acagtgaagg tactgctgcg tatagcactt gatatactgc gaggaaggcc ggaaaaaaaa
      241 gaattaatgc tatttatcat atagataacc ttataagagc tttattggat agtcttattt
      301 atccgtttac atgaaatcta ccttttgtga aattctcgtc aatattttac tcaaaatgat
      361 cggttgtagg aataaactat ttgatattat tacgttttta gagttgtttt caatcaacta
      421 tagatagggc ttggcgccag aatggagaat atttaacgag gaatagaagc tttgtatcag
      481 acgggcttcc ctaaatagag aagttaaata atcaggatga tgctaatgaa tatcagggac
      541 aaggtgagtg tcttcatgtt cagtgaaaca gggtgtcaca aaaaagattg gggagggcta
      601 gagccttctg cacgaaaata gtgatgcgac agatctgaga tggctttgta tccttctgcc
      661 tggttcaggc ctggagcagt ccatgtttta caaaaatact cggcagcgta acgtgttgca
      721 agagcggcat tagacgagga acctgaggtt ccgctcatga ccgataatct catccgaata
      781 aaaatatacc aattggtata ccaagtttat accaagagcc aaaaaagcaa aggggctacc
      841 atttggtaac cccttgatta tttggcggaa gcgcagagat tcgaactctg gaaccctttc
      901 gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg ccacacttcc ggaatgacga
      961 gcactataaa catcccgatg cggcgtgtaa acccctaatt tgtttgtttg cctgaaaaac
     1021 agccaaaagt gcattgatag cgtgaaataa cagcagattg atcatttcat caccatgaat
     1081 tccttctctt ttactcgttt agcaactggc taaacatccc caccgcccgg caaaaagaaa
     1141 aataggtcca tttttatcgc taaaagataa atccacacag tttgtattgt tttgtgcaaa
     1201 agtttcacta cgctttatta acaatacttt ctggcgacgt gcgccagtgc agaaggatga
     1261 gctttcgttt tcagcatctc acgtgaagcg atggtttgcc ttgctacagg gacgtcgctt
     1321 gccgaccata agcgcccggt gtcctgccgg tgtcgcaagg agaagagacg tgcgatatga
     1381 ataacgagga aacattttac caggccatgc ggcgtcaggg cgttacccga cgcagctttc
     1441 tcaaatattg cagtctggct gccacgtcgc tgggattagg cgcgggaatg gcaccaaaga
     1501 ttgcctgggc gctggagaac aaaccgcgca ttccggtggt atggatccac ggtctggaat
     1561 gcacctgctg taccgaatct tttatccgct ccgctcaccc actggcaaag gacgtcatcc
     1621 tttccctgat ttccctcgat tacgacgata ctttgatggc tgccgccgga acccaggcgg
     1681 aagaagtctt tgaagacatc atcgcgcaat acaatggcaa atatatcctc gcagtagaag
     1741 gtaatccgcc gctgggcgag caggggatgt tctgtatcag cagcggtcga ccgtttattg
     1801 agaaactcaa acgtgccgct gccggagcca gcgcgattat cgcctgggga acctgcgcgt
     1861 cctggggctg cgtgcaggcc gcgcgaccta atccgacgca ggcaacgcct atcgacaaag
     1921 tcatcaccga caaacccatt atcaaagtac ctggctgccc gccgatcccg gatgtgatga
     1981 gcgccatcat tacttacatg gtgacctttg atcgcttgcc agatgtcgac agaatgggcc
     2041 gtccattgat gttctatggt cagcgaatcc acgataaatg ctatcgccgc gcccacttcg
     2101 acgccggaga gttcgtccag agttgggatg atgacgctgc ccgcaaaggt tactgtctgt
     2161 acaaaatggg ctgcaaaggg cctaccacct ataacgcctg ttcctccaca cgctggaatg
     2221 atggcgtttc tttcccaatc cagtctggtc acggctgcct gggttgtgcg gaaaatggtt
     2281 tctgggatcg cggttcgttc tacagccgcg tggtcgatat tccgcaaatg ggtactcatt
     2341 ccaccgccga taccgtcggt ttaaccgcgc ttggcgtggt ggcagcggct gttggtgtgc
     2401 acgcagtcgc cagcgccgtt gaccagcgca gacgtcataa ccagcaacct acagaaactg
     2461 aacatcagcc aggcaatgag gataaacagg catgagcact cagtacgaaa ctcagggata
     2521 caccatcaat aatgccggac gccgcctggt ggtcgacccg attacgcgca ttgaaggcca
     2581 catgcgctgc gaagtgaata ttaacgatca gaatgtgatc accaatgccg tctcctgcgg
     2641 caccatgttt cgcgggctgg agatcatcct gcaagggcgc gacccgcgca atgcgtgggc
     2701 gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac gccctggctt cggtttacgc
     2761 catcgaagat gccatcggta ttaaagtgcc ggacaacgcc aatatcatcc gcaacattat
     2821 gctggcaacg ctctggtgcc acgatcatct ggtgcacttc tatcagctcg ccgggatgga
     2881 ctggatcgat gtgttagatg cgctgaaagc cgacccgcgg aaaacctccg aactggcgca
     2941 aagtctctcc tcatggccga aatcatcccc tggctatttc ttcgacgtac aaaaccgcct
     3001 gaaaaaattt gttgaaggcg ggcagttggg gatcttccgc aatggctact gggggcaccc
     3061 gcagtacaaa ctgccgccag aagccaacct gatgggcttt gcccactatc tagaagctct
     3121 cgatttccag cgtgaaattg tcaaaatcca cgcggtcttt ggcggtaaaa acccgcatcc
     3181 aaactggatt gtcggcggga tgccttgcgc cattaacatt gacgaaagcg gcgcggtcgg
     3241 ggcggtcaat atggaacgcc tgaacctggt gcagtcgatt atcacccgca cggcagactt
     3301 cattaacaac gtgatgatcc ccgacgcctt agccatcggt cagtttaaca aaccgtggag
     3361 cgaaatcggc acgggtcttt ctgataaatg tgtcctcagc tacggcgcat tcccggatat
     3421 tgccaacgac tttggtgaga aaagtctgct gatgcctggc ggcgcggtga ttaacggcga
     3481 cttcaacaat gtgctgccag tggatttggt tgatccgcag caggtgcagg agtttgtcga
     3541 ccacgcctgg tatcgttatc ccaacgatca ggtcgggcgt catccattcg atggcattac
     3601 cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat accaacattc agcagctgaa
     3661 tgaacaggaa cgctactcgt ggatcaaagc gccgcgctgg cgcggtaacg cgatggaagt
     3721 ggggccgctg gcacgcacct taatcgctta tcacaaaggc gatgctgcga ccgttgagtc
     3781 ggtcgatcgc atgatgtcgg cgctgaacct gccgctttcc ggtatccagt caacgttggg
     3841 ccgcattttg tgccgcgcgc acgaagcaca gtgggccgca ggtaagttgc agtatttctt
     3901 caacaagctg atgaccaacc tgaaaaacgg caatctcgcc actgcttcca cggaaaaatg
     3961 ggaacctgca acttggccga cagagtgccg tggtgtcggt tttaccgaag cgccgcgcgg
     4021 ggcgttaggc cactgggccg ccattcgcga tggcaagatt gatctctacc agtgcgtggt
     4081 gccgaccacc tggaacgcca gcccgcgcga tcccaaaggg cagattggcg cttatgaagc
     4141 agcgctgatg aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc tgcgtactct
     4201 gcacagcttt gatccgtgcc tcgcctgttc aacacacgtg ctgggcgacg acggtagcga
     4261 gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac agaaaagcga
     4321 caacgttgtc agccactatg tctttgaagc gccagtgcgt atctggcact ggctgacggt
     4381 gttatgcatg gcggtgttga tggtcaccgg ttactttatc ggcaagccgc taccttccgt
     4441 cagcggcgag gcgacgtatc tgttctatat gggctacatc aggttaattc acttcagcgc
     4501 cgggatgatt tttaccgtgg tcttgctgat gcggatctac tgggcttttg ttggcaatcg
     4561 atactcccgc gagctgttta tcgtgccggt atggcgtaaa agctggtggc agggcgtgtg
     4621 gtatgaaatc cgctggtatc tgcttctggc aaaacgtccg agtgccgata taggccataa
     4681 ccccatcgcc caggcggcga tgttcggcta tttcctgatg tcggtcttta tgatcatcac
     4741 tggctttgcg ctgtacagcg aacacagcca gtacgctatt tttgcgccgt tccgttatgt
     4801 ggtggaattt ttctactgga cgggcggcaa ctcaatggac attcacagct ggcatcggct
     4861 ggggatgtgg ctgattggcg catttgtgat cggtcatgtc tacatggcgc tgcgtgaaga
     4921 catcatgtcc gacgacacgg tgatctccac tatggtcaac ggctaccgta gccacaaatt
     4981 tggcaaaata agtaacaagg agcgttcatg agcgagcaac gcgtggtggt aatggggctg
     5041 ggcaacctgc tgtgggccga tgaaggcttc ggcgtgcggg tggcggaacg gctgtatgcc
     5101 cattaccact ggcccgagta tgtggagatt gtcgatggcg gtactcaggg actgaacttg
     5161 ctggggtatg tcgaaagcgc cagccatctg ttgattctcg atgccattga ctacgggctg
     5221 gaacctggaa cgctgcgaac ctatgccgga gaacgcattc cggcttatct cagcgcgaag
     5281 aaaatgagcc tgcaccagaa cagtttctcc gaagtgctgg cgctggcaga tatccgcgga
     5341 catctgccag cacatattgc cctcgtcggt ctgcaacccg caatgctcga cgactacggc
     5401 ggcagcctga gcgaactggc gcgggagcaa ctgcccgctg cggaacaatc ggcgctggcg
     5461 caactggctg cgtggggaat tgtgccgcaa ccggctaatg aatcgcgctg tctcaattat
     5521 gactgtctgt cgatggaaaa ttacgaaggc gttcgcttgc gccagtaccg gatgacacag
     5581 gaggagcagg gatgagcaac gacacgccat ttgatgcgtt gtggcaacga atgctggcgc
     5641 gcggctggac gccggtcagt gaatcccgtc ttgacgactg gcttacgcaa gcgccagacg
     5701 gcgtggtgtt attaagcagt gacccgaaac gtacgccaga ggtcagcgat aatccggtaa
     5761 tgattagcga attactgcgc gagtttcccg actatacatg gcaggtggcg attgctgacc
     5821 ttgagcagag cgaagccatc ggcgatcgct ttggcgtctt tcgctttccc gccactttag
     5881 tgtttaccgg cggaaactat cgcggtgtgc tgaatggtat tcacccgtgg gcggaactga
     5941 taaacctgat gcgcgggctt gtcgaaccgc agcaggagcg tgcctcatga gcgaaacttt
     6001 tttccatctg ctggggccag gaacgcaacc gaacgatgac agtttcagca tgaatccact
     6061 gccgatcacc tgtcaggtga atgatgaacc gagtatggcg gccctggagc aatgtgctca
     6121 cagcccgcag gtgattgcgc tgttaaacga gttacaacat caactaagcg aacgccagcc
     6181 gccgttgggc gaggtgctgg cagtcgatct gttaaatctc aacgccgacg atcgtcactt
     6241 tatcaatacg cttctcgggg aaggggaagt gtcagtgcgc attcagcagg ttgacgacag
     6301 tgaaagtgaa atacaggagg cgatcttctg cggattatgg cgggtgcgca gacgtcgcgg
     6361 cgaaaagttg ctggaggaca aactggaggc tggctgcgcg ccgctggcgt tgtggcaggc
     6421 ggcaacgcaa aatctcttgc cgacagattc gctgttaccg ccgcccattg atggcctgat
     6481 gaatggccta ccgttggcgc atgagttact ggcacatgta cgtaaccccg acgcgcagcc
     6541 gcacagcatt aatctgacgc aattacccat cagcgaggct gatcggcttt ttctctcacg
     6601 tctctgtggg ccgggaaata ttcagattcg taccattggc tatggcgaga gctatatcaa
     6661 cgccacgggg ttacgccatg tctggcattt acgctgtacg gacaccttaa aaggcccgtt
     6721 actggaaagt tatgaaatct gcccaatacc ggaagtggtg ctggcagcgc cagaagattt
     6781 ggtcgactct gcgcagcggc ttagcgaggt atgtcagtgg ctggcggaag ctgcaccgac
     6841 gtaaaaagac ggtaagtatc gctttcaggc ttatgaatat cgcaatcggc gaatacctct
     6901 ggtcgtaaag tttcaggata aagagggaga tctaccatta tcgggttatt tttctctctt
     6961 cgcctacagg agtgcgcatg tgggatgtca ttgatttatc gcgctggcag tttgctctga
     7021 ccgcgctgta tcacttttta tttgtacccc ttaccctggg gctgattttt ttgctggcta
     7081 tcatggaaac catttacgtg gtcaccggca aaacgatcta ccgcgatatg acgcgcttct
     7141 ggggtaagct cttcggtatc aactttgctc ttggcgtggc taccggcctg acgatggagt
     7201 ttcagtttgg tactaactgg tcattctatt ccaactatgt gggcgatatt tttggcgcac
     7261 cactggcgat ggaagcatta atggccttct tcctcgaatc cacctttgtc gggctgttct
     7321 tcttcggctg gcaacggctg aataaatacc agcacctgct ggtgacgtgg ctggtggcgt
     7381 tcggttcaaa tctctctgcg ttgtggatat tgaatgccaa cggttggatg caatacccga
     7441 ccggtgcgca ttttgatatc gacaccctgc gtatggagat gaccagtttc agcgagttgg
     7501 tctttaaccc ggtcagccag gtgaaatttg tgcacaccgt aatggcgggc tacgtgaccg
     7561 gggccatgtt tattatggcg atcagcgcct ggtatttact gcgcggacgg gagcgcgatg
     7621 tcgcattacg ctcgtttgcc atcggttccg tcttcggtac tctggcgatt atcggtaccc
     7681 tgcaactcgg agacagttct gcgtatgaag tcgcgcaagt acaaccggta aaactggcgg
     7741 cgatggaagg ggagtggcaa acggaacctg cacctgcacc gttccatgtg gttgcctggc
     7801 cggaacagga tcaagagcgt aacgcctttg ctatcaaaat tcccgcgctg ctagggatcc
     7861 tcgccactca ctcattagat aaacccgtgc cgggtctgaa gaatttgatg gctgaaacct
     7921 acccacgctt gcaacgcgga cgtatggcct ggctgttaat gcaggaaata tcgcaaggca
     7981 atcgtgagcc gcatgtgttg caggcattcc gggagctgga aggcgacctt ggttacggca
     8041 tgttgctctc ccgctatgcg ccggatatga atcatgtcac agccgcacag taccaggcgg
     8101 cgatgcgtgg cgcgatacct caggttgcgc cggtattctg gagtttccgc atcatggtgg
     8161 gctgtggttc cctgctgcta ctggtgatgc tgattgcgct tgtccagacg ctgcgtggca
     8221 aaatcgacca gcatcgctgg gtgctgaaaa tggcgctctg gagtttgccg ttgccgtgga
     8281 ttgcgattga agccgggtgg tttatgaccg agtttggtcg tcagccgtgg gcgatacagg
     8341 acatcttacc gacatactcc gcgcactccg ctttaaccac aggacaactg gctttctcac
     8401 tgatcatgat cgtagggctt tacaccctgt tcttaatcgc cgaagtctac ctgatgcaga
     8461 aatatgcccg tctggggccg agcgcgatgc agagtgaaca accgacgcag caacaggggt
     8521 aaaggagaaa atcatgtttg attatgaaac attgcgcttc atctggtggc tgctgattgg
     8581 cgtgatcctg gtggtcttta tgatctccga cggatttgac atggggatcg gctgtttgct
     8641 gccgctggtg gcgcgtaata atgatgaacg ccggatagtg attaatagcg ttggtgcgca
     8701 ctgggaaggc aatcaggtct ggttgatcct cgcaggtgga gcattatttg ccgcctggcc
     8761 cagagtgtat gcagcggcgt tttccggctt ttatgtggcg atgatcctgg tgctgtgctc
     8821 actgttcttc cgcccgctgg cctttgatta tcgcggaaaa atcgccgatg cacgctggcg
     8881 taaaatgtgg gatgccggtc tggtcatcgg cagtctggtg ccgccggtag tcttcggtat
     8941 cgccttcggc aacttgctgc tcggcgtgcc gtttgccttc acaccgcaat tacgcgtgga
     9001 gtatctcggc agcttctggc aactgctgac gccattccct ttattgtgcg gattactcag
     9061 ccttgggatg gtgattttgc aaggtggcgt ctggttacaa ctgaaaactg ttggtgtgat
     9121 tcatctgcgt tcacagctgg cgactaaacg cgccgcacta ctggtgatgc tgtgcttttt
     9181 gctggcgggt tactggctgt gggtcggtat tgatggcttt gtactgctcg cccaggatgc
     9241 taacggtcct tccaatccgt taatgaaact ggtagcagtg ctacctggtg cctggatgaa
     9301 taatttcgtc gaatcgcccg ttttgtggat cttcccgctg ctggggttct tctgcccatt
     9361 gctgacggtg atggcgattt atcgtggtcg cccgggttgg ggatttttaa tggcgtcatt
     9421 gatgcaattt ggcgtgattt tcacggcagg catcacgctg ttcccctttg tcatgccgtc
     9481 aagcgtgagt ccaatctcca gcctgacgtt gtgggacagc acttccagtc agctgacgct
     9541 gagcattatg ttggtaatcg tgctgatatt tttgcccatt gtgttgctct acactctctg
     9601 gagctactac aaaatgtggg gacgcatgac gacagaaact ctccgccgta acgaaaacga
     9661 gctgtactaa ggagcaggga caatgtggta tttactttgg ttcgtcggca ttttgttgat
     9721 gtgttcgctc tccacccttg tgttggtatg gctggacccg cgattgaaaa gttaacgaac
     9781 gtaagcctga tgcggcgcat tagcgtcgca tcaggcaata atgtcggata tgaaaagcgg
     9841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttttgattc cgttaacccc
     9901 gcaatctgca ttcgctcaga gtgagtcgga gctgaagctg gaaagtgtgg tgattgtcag
     9961 tcgtcatggt gtgcgtgccc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    10021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    10081 cgcttatctc ggacattacc aacgccagcg tctggtggct gacggattgc tggcgaaaaa
    10141 gggctgcccg cagcctggtc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    10201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    10261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggcg tttgccaact
    10321 ggataacgcg aacgtgactg acgcgatcct cagcagggca ggagggtcaa ttgctgactt
    10381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt ttccgcaatc
    10441 aaaattgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    10501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    10561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    10621 aaggatcacc gattcacacc agtggaacac cttgctaagc ctgcataacg cgcagtttta
    10681 tttgctgcaa cgcacaccag aggttgcccg cagtcgcgcc accccgttgt tggatttgat
    10741 catgacagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    10801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    10861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    10921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    10981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    11041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggccgg
    11101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    11161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    11221 agaccggaaa aggcgttcac gccgcatccg gtcactttac cttcagtttt actctttctc
    11281 ggagtaacta taaccgtaat agttatagcc gtaactgtaa gcggtgctgg cgcgtttaat
    11341 cacaccattg aggattgcgc ctttaatatt aacgccagcc tgttccagac gctgcattga
    11401 caagctcacc tctttggcgg tgttcaggcc aaaacgcgca accagcaggc tggtgccgac
    11461 agagcgtccc acgaccgcag catcgctcac cgccagcatc ggcggcgtat cgacaatcac
    11521 cagatcgtaa tggtcgttcg cccaatccag ta
//
LOCUS       DAHBHC010000119        11272 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHBHC010000119 DAHBHC010000000
VERSION     DAHBHC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.120"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(52..324)
                     /locus_tag="M2A35_004493"
     CDS             complement(52..324)
                     /locus_tag="M2A35_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7200704.1"
                     /translation="MKGNDYDALRELIAQNAIARNLGVTPQAVNQWFSKRTIPARFVL
                     RVCEVVEWKVTPHGLRPDLYPHPEDAIPDLLRRSHTATNAAVMKNE"
     gene            459..1148
                     /locus_tag="M2A35_004494"
     CDS             459..1148
                     /locus_tag="M2A35_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCB7200705.1"
                     /translation="MKNLKSTENRITKLLQEKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRGNRLAQLSEISGYPQSWFLGEITSRQFSSKEKHQARTDSVVFNVLDVEFSCGDGT
                     HVRGDFIDVVRSIELDPEYARRLVGNRAFQNIEIGNARGDSMAPTIAPGDLLFLDKTV
                     NYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQSWSIEKDALELLYIQSKVI
                     SSVPFNINRFG"
     gene            1295..1723
                     /locus_tag="M2A35_004495"
     CDS             1295..1723
                     /locus_tag="M2A35_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7200706.1"
                     /translation="MKDLTTAEISNLNHFEKESFALNIANLVGAARTFHLIDGDRSKE
                     LGFEVMEFVHEYALSATYPLHNHIINKQPNKIRTQREACGLTTAELARLLDLDEEIII
                     QWESGEYEPTISMLIPLANVLGCDPMLLLTDAYKQNQEEA"
     gene            1725..1916
                     /locus_tag="M2A35_004496"
     CDS             1725..1916
                     /locus_tag="M2A35_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200707.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            1913..2104
                     /locus_tag="M2A35_004497"
     CDS             1913..2104
                     /locus_tag="M2A35_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200708.1"
                     /translation="MITPLNILEEVAAQIKENTSMLEFIFKNSPDQGETDDYLCCLIR
                     SMNKTCEMAYEYIETLRNE"
     gene            2289..2648
                     /locus_tag="M2A35_004498"
     CDS             2289..2648
                     /locus_tag="M2A35_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200709.1"
                     /translation="MACTTIYKDRRRMVTAALRRMQRKTGSNLLVVDLPDGEITTIEI
                     TEQFMNQLLLRFEGLTRVEYGRTKGEATIRTAYQNAIGINQHTEYLTESGKLIVDGLL
                     KEVVDYVKEKYISGGIN"
     gene            2648..2857
                     /locus_tag="M2A35_004499"
     CDS             2648..2857
                     /locus_tag="M2A35_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200710.1"
                     /translation="MSDLSPLIHEKVQIIMTIENGQVINTRKVHNNEMIASMDTFLWI
                     AKKAGYQVIAPDLEEIGDINSNTNS"
     gene            3084..3662
                     /locus_tag="M2A35_004500"
     CDS             3084..3662
                     /locus_tag="M2A35_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200711.1"
                     /translation="MINSTITPEPTLTGIRFGNRIIGYSTAVRQIDNGNYDKRIPDGL
                     DLIACIMEGIESGWFMPCIEKQIILWRWLVVAVFIAEEQEKNGTVDVQNDKGGVDTAV
                     VYTGKPGSISIYPWSERFALANHIEAGAIEKYGPDVGQQMALRMYQDMVVTDEKCGFR
                     LSAMGREGLNILHDSFIELIQKDGMPDMPVMH"
     gene            3674..4420
                     /locus_tag="M2A35_004501"
     CDS             3674..4420
                     /locus_tag="M2A35_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCB7200712.1"
                     /translation="MNIVTINNKQLPAVEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMTDAKCRNLVNAYSIPTDTHEFMTPDGLLSRRAIVELEPFMKAF
                     HQMMSEAEPRGTRWYHPKMGLFQVIGWEGK"
     gene            4420..4758
                     /locus_tag="M2A35_004502"
     CDS             4420..4758
                     /locus_tag="M2A35_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200713.1"
                     /translation="MSLITQNFRLNALANQYAAALHNHISATSGGHFLINVNGQSIRV
                     NIIGGVKGIRDLIDGYALKAIKENYPQWEAVGIQLLSKCVRGNELTQSGKEIWQSMVN
                     DMSDTVMGNM"
     gene            4759..4980
                     /locus_tag="M2A35_004503"
     CDS             4759..4980
                     /locus_tag="M2A35_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200714.1"
                     /translation="MKIQYQDYGAVANIVITSSCLTLQKHNRVVDAVLFNVQGISENR
                     SGFFWVKSSLSGKTRDILRAYKIVQREAK"
     gene            5084..5713
                     /locus_tag="M2A35_004504"
     CDS             5084..5713
                     /locus_tag="M2A35_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200715.1"
                     /translation="MGKIKNPVVLIHKRENSDSYAVAITCGSQNYHDAVLMASMELDM
                     SDDDVDTWSKTGYYMAEEIERLKQALSAAENNLIDSECLVAELSKAKDHAYGLVDTYD
                     WQRQRLHAAAEKVIKWCRQEAEHRTGDPEKAENYACVKELRDALNFCKSSGDIKKKGL
                     TITLPDTTSKAFWSGTGKSAKFHLETYKRWVKEAIKRDCVIAGIGVEVK"
     gene            5801..6043
                     /locus_tag="M2A35_004505"
     CDS             5801..6043
                     /locus_tag="M2A35_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCB7200716.1"
                     /translation="MFALIQRGQIYTDSAGYPIKIIRCINNTVLYRRMDGRTLSVKIN
                     DFNELFERINHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            6047..6193
                     /locus_tag="M2A35_004506"
     CDS             6047..6193
                     /locus_tag="M2A35_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200717.1"
                     /translation="MNKAFEQWVHQRYGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            6366..7538
                     /locus_tag="M2A35_004507"
     CDS             6366..7538
                     /locus_tag="M2A35_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCB7200718.1"
                     /translation="MGKKEQGSLSLPRGVTIRQHKTDSTLVITFTYKGVLCREPLSRM
                     EVNARGVKYAERLLGEIKNQIIDGTFEYAKYFPNSQKLKLFGGVKKNKNIKSYLDEYL
                     IICQNRNLSPSTIRGYEKCLSALSALHKFHVSELTPAVLKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVLDSTPGSDDYEVDPFTPAETAAIYDACH
                     YPEWRNLFRFAFNTGLRSSELCALRWTDIDFIGNTAHVQTASVVGVIKSTKTKAGTRK
                     VQLNSEALAALQAQKPYTALKSDFIFSDPKTRAPWANADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRHYGSYLAEYDGKTAISAAP"
     gene            7863..9092
                     /locus_tag="M2A35_004508"
     CDS             7863..9092
                     /locus_tag="M2A35_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7200719.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            9131..9547
                     /locus_tag="M2A35_004509"
     CDS             9131..9547
                     /locus_tag="M2A35_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200720.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(9619..11013)
                     /locus_tag="M2A35_004510"
     CDS             complement(9619..11013)
                     /locus_tag="M2A35_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012440716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200721.1"
                     /translation="MIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSYWE
                     DVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAE
                     YAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLND
                     SLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRCRN
                     IDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLPSN
                     NDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPH
                     HVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDY
                     MVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQY
                     SSRIGVDYVSMMLP"
BASE COUNT         3536 a         2368 c         2454 g         2914 t
ORIGIN      
        1 cctagatggc atctctgtct gaacgtaatt ctgggacata caagcccttc atcattcgtt
       61 tttcataact gccgcatttg tggctgtgtg acttctacgt aacaaatcag gaattgcatc
      121 ttcaggatgg ggataaagat ctggtcttaa cccatgagga gtaactttcc attccacaac
      181 ttcacataca cgcaagacga aacgagcagg aattgttctt tttgaaaacc attggttcac
      241 agcttgcggc gtaacgccta gatttcgcgc tatggcattt tgtgcaatta gctcacgaag
      301 tgcgtcgtaa tcatttcctt tcatcgcaac ccaccaatct aaactttata aattcaagat
      361 tacatcaagt ttaaattaac atgcaagtcc acaaaagatc gaatacacta aaatcaagta
      421 aaaatttatc gaaaccaagt aaccagcaag gattaacaat gaaaaactta aagagcactg
      481 aaaatcgaat tacaaagctg ctacaggaga aaggctggag ccaagcagag ctggcccgaa
      541 aattaggtgt cagcgcacag tccgtacagt actggacaac aggaaaaaca tttccaaggg
      601 gaaaccggct tgcccagtta tccgaaataa gtggttatcc gcaatcgtgg tttttaggag
      661 aaatcacgtc acggcagttc tcttcaaagg aaaaacatca agcaagaact gacagtgtgg
      721 tctttaacgt gctcgatgtt gagtttagtt gcggcgatgg aacacatgtc cgtggggact
      781 ttatcgatgt agttcgctca atcgagcttg atcctgaata tgcccgtcgc cttgttggca
      841 atagagcatt tcaaaacata gagattggta acgcaagagg agatagtatg gctccaacaa
      901 tagccccagg agatcttctt tttctcgata aaacagtgaa ttactttgat ggtgatggca
      961 tttatgcttt ttgctttgat ggtgaatgct acgtcaaaag attacaaaag ataggaagta
     1021 aaatcatggt gttatctgat aacccaaatt accaatcctg gagcattgaa aaagatgctc
     1081 ttgagctgct ttatatccaa tcgaaggtaa tctcatctgt tccattcaat ataaatagat
     1141 ttggttaatt attgattatt aaagaaaaat ttacaaatca agtaaaagag acgcagaaat
     1201 caattttttc ttgacaactc catcaatcaa gatctaacat cacaccatca attttaactt
     1261 gatttattgt taattttaaa ttgatggtga ttcaatgaaa gatctgacaa ccgctgagat
     1321 atcgaactta aaccacttcg aaaaagaatc ctttgcatta aatattgcaa atctggtagg
     1381 tgctgctcgt actttccatt taattgatgg tgatcgctct aaagaattgg ggtttgaagt
     1441 catggagttt gttcatgaat acgccctgtc tgctacttat ccattacata atcacataat
     1501 caacaaacaa ccaaataaaa tccgcaccca acgcgaagcc tgcggcttaa caaccgccga
     1561 actcgccagg ctgctcgatc tcgatgaaga aattatcatc cagtgggaaa gcggagagta
     1621 cgaaccaacc atcagcatgc ttatccctct ggctaatgtt cttggctgtg atccaatgtt
     1681 gttattgact gatgcttata aacaaaatca ggaggaagca taagatgaaa atgttcaaag
     1741 gccttaccaa cgaaccggag acagcttttc atcacattgc cgtactgctt gaagcggggt
     1801 taatcatttc ggcttccggt gatgaagaat gtgatgaact ttcggatgat atctttttac
     1861 tggcacaaca atacgccaga agcgcatgcg atgcatttaa ggagcaaaga acatgataac
     1921 tccattaaat attcttgagg aagtggcggc acaaataaaa gagaatacat caatgcttga
     1981 atttatattt aagaattcgc ccgaccaagg agagacagac gattatttat gctgtctcat
     2041 tcgctccatg aataagacct gtgaaatggc ttacgagtat atagaaacac tacgcaacga
     2101 ataaagaaca ctcagtaaaa aatcacacat aaaaacatga cggcctctgg tcgtggattt
     2161 ccacaaccag aaaataatgg tgatgcatga aaaatagaaa cgcttataaa accgcattat
     2221 taatggctaa tgcaggatac tggtcagttg caatactgtt tctcagaaaa gcatacggga
     2281 aataacaaat ggcatgtaca accatttata aagatcgtcg ccgcatggta acagcggcac
     2341 tccggcggat gcaacgtaaa acaggtagta atctgctcgt cgttgacctc ccggacggag
     2401 aaataacaac gatagaaata actgaacagt tcatgaacca gttgttatta cgatttgagg
     2461 gtcttacccg tgttgaatat ggacggacga aaggagaagc aacaatccgt accgcatacc
     2521 agaatgccat cggtattaat caacacacgg aatatctcac tgaatcaggg aaactgatag
     2581 tggacggcct tttaaaagag gttgttgact acgtaaaaga gaaatatatc agtggaggaa
     2641 ttaactgatg tctgatttat cccctcttat acacgaaaaa gtacagatca ttatgacgat
     2701 tgaaaatggt caggtaataa atacccgtaa agttcacaat aacgagatga ttgccagcat
     2761 ggataccttc ttatggatag caaagaaagc gggatatcag gtaatcgctc ctgatctgga
     2821 ggaaatcggt gacattaaca gcaatacgaa ttcctgagtg ggtgcacctg caggcggtcc
     2881 gtgttctccg ccagttcaga gccagacgaa ttcatccctg tcgtatgcac ggctccggaa
     2941 acctgagtct gagggttaat cgccgctggc ggctgctgtc ccgagacggc ggccagaact
     3001 gggaagtaat gagccatgaa cgatacagca aagttaagga ccggaaatga acaataaacc
     3061 ttcaaaccaa aaggagagta ttgatgatta attcaaccat tacccctgaa cctacattaa
     3121 caggcatccg ttttggtaat cgaattattg gttattcaac tgcggttcgc cagatcgaca
     3181 acggtaacta tgacaaacga attccggacg ggctcgatct gatagcttgc attatggaag
     3241 ggattgaaag cggctggttt atgccgtgta ttgaaaaaca aatcatccta tggcgctggc
     3301 ttgttgtcgc cgtgttcatt gctgaggagc aggagaagaa cgggactgtc gatgttcaga
     3361 atgacaaggg cggcgtagat acagccgttg tatacacagg aaaacccggt tcaatcagca
     3421 tttatccatg gtctgagcgc ttcgcgctcg ctaaccatat cgaggcgggc gcaattgaaa
     3481 aatacgggcc tgacgttggc cagcagatgg cgctgcgtat gtatcaggac atggttgtaa
     3541 ctgacgagaa atgcggattc aggctgtcag cgatgggccg ggagggactc aacatactac
     3601 atgacagctt cattgaactg attcagaaag acggtatgcc agacatgccg gttatgcact
     3661 gaggtggacg aaaatgaaca tagtaacgat caataacaaa cagcttccgg cagtcgaata
     3721 tcgcggtcag cgtgttgtga cgctggcgat gattgatgaa gtccaccagc gaccggaagg
     3781 aacagcgcgt gcagcattca accgaaaccg tgagcatttc atcagcggtg tggattatgc
     3841 cgaattaggt gcggacgtaa tacgtacgga cctcccggaa ggaacattct ctaaatttgc
     3901 accatcaggg attgtacttt ttgaatcagg ttacctgatg ttgacgaagc cattcaatga
     3961 cgcccttgcc tggcaggttc agcgcgaact ggttaacagc tacttccgca ctcgcacacc
     4021 gctaacggaa atcgagatga tcgctgcgat agccgccgac gctgttcgtc aacagaagcg
     4081 cctgaatcat gtggaagaac agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg
     4141 cggaaatatg cgcgccggat atgtcggtta tcgccaggtg gtcgccaaaa gcggaatgac
     4201 tgatgctaag tgccgcaatc tggtcaatgc ctacagcatc ccaaccgata cgcacgaatt
     4261 tatgacacca gatggtcttc tttcgcgccg ggctatcgtg gagctggagc cattcatgaa
     4321 agcatttcat cagatgatgt cagaggcaga gccacgtggt acccgctggt atcacccgaa
     4381 gatggggctg tttcaggtga ttggttggga gggtaaataa tgtcactcat tacacaaaat
     4441 ttccgcctca atgcgctggc aaatcagtat gcggcggcac tgcacaacca catatccgcc
     4501 accagcggtg gtcatttcct gattaatgtc aatgggcaat ccatacgagt aaacatcatc
     4561 ggtggggtta aaggcatcag ggacttaatc gatggttatg ctctgaaggc tatcaaagaa
     4621 aattaccccc aatgggaagc cgttggtatt caactgttaa gcaaatgcgt tcgtggaaac
     4681 gagctgacgc aaagcggcaa agaaatctgg cagagcatgg tcaacgacat gagcgatacc
     4741 gttatgggga acatgtaaat gaaaatccaa taccaggact atggcgccgt agcgaacatc
     4801 gtgatcacca gttcctgcct gacattgcaa aagcataacc gcgttgttga tgcggtgctg
     4861 tttaatgtac aggggatctc tgaaaaccgc agcggctttt tctgggttaa gtcatcgttg
     4921 tcaggaaaaa cacgcgacat cctgcgcgcc tacaaaatcg tacagcggga ggcaaagtga
     4981 tgaagtcatt cctcctgtcc atgctgttta gtctgctgct ggcgtccgtc gttttcggta
     5041 cgctgattga atataaattt ttaatgaatt actgaggtgc ggggtgggca agatcaaaaa
     5101 cccagttgta cttatccata agcgagaaaa tagtgactcc tacgccgttg caatcacctg
     5161 cggtagccag aactatcacg acgccgttct gatggcgtcg atggaactgg atatgagtga
     5221 cgatgatgtc gatacctgga gtaaaacagg ctattacatg gctgaagaaa tcgagcgact
     5281 gaaacaagca ctttccgctg cggaaaataa tctgattgat tccgaatgcc tcgtagctga
     5341 attaagcaaa gcaaaggatc acgcatatgg gctggtcgac acatacgact ggcaacgtca
     5401 gcgtctccac gcagccgctg agaaagtcat caaatggtgc agacaagaag ctgaacaccg
     5461 taccggcgat ccagagaaag cagaaaacta tgcgtgcgtt aaagaactac gcgacgcatt
     5521 aaatttttgc aaaagttctg gagacatcaa gaagaaaggc ctgaccatca ccctgcccga
     5581 taccacatca aaggcattct ggagcggtac cggaaaaagc gcaaaattcc atctggaaac
     5641 ctataaacgc tgggtgaaag aagctatcaa acgggattgt gttattgccg ggatcggcgt
     5701 ggaggtgaaa tgatgaattg gcctgaagca ttcaccgctg taggagttgc aatagcggtg
     5761 gcatttattc tgtattcgct tttccgctgg ggataaagga atgttcgctc tgattcaacg
     5821 tggtcagata tacaccgata gtgctggcta cccgataaaa attattcgct gcataaacaa
     5881 cactgtgttg tacagaagaa tggatgggag aacactgtcg gtaaaaataa acgattttaa
     5941 tgaactgttt gaacgaatca atcaccagga ataccgacaa attctggcag aaacagaaca
     6001 ggaagctcat ctgaaaaaat tacgggccat gaaaaggaag taaagaatga ataaagcatt
     6061 tgaacaatgg gtccaccagc gttacggcaa tcgctatgac ctgacacgag atgttgacgg
     6121 tttctactgt cgtgaaattg tgaaacgaat gtttgaagtg tggtgccact gccgtgggct
     6181 gagtgttgtg tgaggtgaac tttggggctg gattgcgtac ctatatcaac ctactgccgc
     6241 aacgcgggag aaacggttga tgccgttaac aaacggatac aaagaggaat atggaaagaa
     6301 ggggtacatg tattaaaagt cgacggggtt aaagaacgtt gggtcgactt aatggagatt
     6361 acaaaatggg caagaaagaa caaggatcat tatctctccc tagaggagta actattcggc
     6421 agcataaaac tgatagcact ctggttatca cttttacata taaaggggtt ctgtgtcggg
     6481 agcccctctc cagaatggaa gtaaacgcgc gcggtgtgaa gtacgccgag cgtctgctgg
     6541 gagaaataaa aaatcagatt atcgatggta ccttcgaata tgcaaaatac ttccccaact
     6601 cccaaaagct gaagttgttc gggggagtga aaaaaaacaa aaatataaaa tcttacctag
     6661 atgaatatct gattatctgc cagaaccgta acctgtcacc atcgacaatt agaggttatg
     6721 aaaaatgcct gtcggcgctg tcagcattgc ataaatttca tgtatcagaa ctgacacctg
     6781 cagtactaaa aaactggatc gccagcagaa aaacaaaact gaaaacgatc cgcaataatc
     6841 tttcgtttct gcgcagcgcc atagatgaag cggtgaccga tggtttgtta acaataaatc
     6901 ctgtcacgct agtcagcgcc agccgatatc acgtattaga cagcacacca ggcagtgatg
     6961 attacgaggt cgatcccttt acgccagcgg agacagcagc aatctatgac gcctgtcatt
     7021 acccggaatg gcgaaatcta tttcgcttcg cctttaatac cgggctgcgc agctcggaat
     7081 tgtgcgcatt gcgctggaca gatatagact tcatcggcaa cacagcgcat gtccagactg
     7141 caagtgtagt cggggtaatc aaaagcacca aaacaaaagc cggcactcgc aaagtacaac
     7201 tgaacagcga agcgctggcg gctcttcagg cacaaaagcc ctacacggcg ctaaaaagcg
     7261 actttatatt cagcgatcca aaaactcgcg ccccttgggc aaacgcggac gcgatccgaa
     7321 aaaaagcctg ggtgccgacc cttaaaaagg ctggtgtacg ctatcgcaat ccgtaccaga
     7381 cacgccatac gttcgccact cggcacatta gccagggcgt taacctgttc tggcttgctg
     7441 gtcagatggg gcataaagga ccggaaatgc tgttcagaca ttacggctct taccttgcag
     7501 aatacgatgg aaaaacggcg atttctgcag ccccgtaacg gcaaaatatt tcaaaatgtt
     7561 gtacagaatc aggacgttac agggacaaca atatgtacgt aaaatgcaca taattccttg
     7621 tttgaaaaaa taaatcgttc atattcaata aattggatta atcacgatca cgggttcaac
     7681 tcccgccagc tccaccactt tttagttgtt tgaagttcaa tgaagtctac taagcccaca
     7741 cagcacaagc tctgcgggct tttttacgtc tattgtcgtc cagtgagaat tgctgagaac
     7801 tacgagttat ggcaccctga atgggaccca ctaagaaggg tccaaaaacc gagggtccca
     7861 aaatggcaaa aatcgctaag aagctcactg acactgaaat caaaagcacc aagccagccg
     7921 ataaagaaat caacttgttt gacggtgatg gtctgattct acgaatcgct cctttggcga
     7981 aaggaggcaa gaaaaattgg tatttcaggt atgcagtacc agtgagcaag aaaagaacca
     8041 aaatgagcct tgggacatat cctcacctta cccttgcaag agccagagcc ttacgtgatg
     8101 aatatctctc ctttctggca aatggtgttg atccccaaat ccataacaac gataaggcga
     8161 aggcattaaa gagtgctact gagcacactc tccaagccgt agcgcggaaa tggttagatg
     8221 agaaggtaaa gacatcaggt atctcacaag accatgcagc agacatctgg cgcagcttag
     8281 agagaaatgt ctttcccggt ctgggtaatg tccctatcaa tgagatccga cctaagctct
     8341 taaaacaaca ccttgatcct attgagcaac gaggtgtatt ggaaactcta cgccgtatca
     8401 tttcacgtct gaatgaaatc ttccggtggg cagctactga agaacttatt gagttcaacc
     8461 cggctgacaa ccttggtcaa agattcagta aaccaaaaaa gcaaaatatg cctgcccttc
     8521 ccccaagcga attgccaagg tttatggaat ctttgacgaa tgcgtcaatc cggttggaaa
     8581 cacgtatgct aattgaatgg caattgttga catgggttcg tccgggtgaa gccgttcgcg
     8641 caaggtggtc tgatattgat acaaccaaca gcatttggaa cattcctgct gatttcatga
     8701 aaatgaaaaa gcttcacaaa gttcctttga gtaaagaagc tttgcgcatc cttgaattaa
     8761 tgaaatcaat aagtgggcat agagaatggg ttttccccag cataaaagcg cctcttaatc
     8821 atatgcatga acaaacagcc aacgcagcta tcatccgaat ggggttcgga ggcgagcttg
     8881 tagctcacgg tatgcgttct attgcacgaa cagcggcaga ggagtctggt aaattcagag
     8941 ctgaagttct tgaggcagcg cttgcccact cgaaaaaaga tgaaattatc gcagcataca
     9001 atcgtgcaga atatctgata gagcgacaga gtttgatgca atggtggagt gattacgttc
     9061 aagctcaaag atcaaatgct ctggtagcct aagtatcaga atagctaata taatcctgaa
     9121 ggtaaagaaa atggaaaccc tattcaaagt ttttgaaaaa tttagttcca gaccactttt
     9181 ttttattttt ttcggactct cactttgtga attttttcag aaacaatctg ttctgatgaa
     9241 tccatcagca gataacatcg cgaaattatt cgcagccatg atattagttg ttttttttac
     9301 ttggggattt gaatggctaa tcttcaagtt caatgtaaac cttgaacctc atgatcaagg
     9361 cgatattgga ccaacaattg gaacggctac tttagctgta tacttagttt atgcctttca
     9421 ctttctcagt gaaaatcctg aagcattaaa tttaaagtta ttaactaact ctggctttat
     9481 atacagcaca actctattat tattctcatt agaatgcatg aagcttagaa gacttaaaca
     9541 aaaataaaca acatcattgt gatgataaat ataaaatagg catggcgaaa aaaaatcacc
     9601 acgcctaaaa tataataatt atggtagcat cattgataca taatccacac caatccttga
     9661 gctatactga gacgctatag cctgatatct ttctgcataa ccagttctca gttgagattt
     9721 aagtttgagt cggacaggaa cattttgcac gttgccatcc atattactta aaaacacggc
     9781 agaaataata tttttttctt cgccatcaac tgttgttcca tgattcaaca ccaccatata
     9841 atcaacaaca ggaagcgttt tatccccttc gaaaatagag agatattttc tttgattttt
     9901 atgcattaca tatatatatt tcgaatgttc agcaaatggc aatgctttac tctgactggc
     9961 gttaaaaagc tccagaactt taatgagcct gtgcggactt aatcttacat ggtgagggtc
    10021 gttaccctga gtaggaacca aatcacatgc cgcagataca cataaatacc atttgttcga
    10081 ctctgtatca aagaaaatag tgccagtaga aatatgacca tcttcaaaat tctttgaaga
    10141 caaattcata tttaaagcat gatacatttc gtgataagta tcattatttg atggcagatc
    10201 catttttgaa gagcaatatt ggagcaatgc agcaactccg ctgttagcgt attcatttga
    10261 atagctatca aaaacacttt tgataaattc atccagcgta ttattatttt taagtctttg
    10321 ataaagctct tctgataaat taccaaatac aaagtcaata tttctacatc taatatcagg
    10381 cgagtctgat tttaatatct catttaacca cgcagcttga ccgtaatgat cgttagccaa
    10441 atgatttaca aaagataaag cctcagcttc gattgcattc tgaatttcag attttattaa
    10501 ctgataataa gatggtttcc attcaatgag agaatcattg agagtttgcc aaatcctatc
    10561 tccatcgttt tcatgatcat cttgaacctt atgaaatagg gagacaaaga tattaccaca
    10621 ttgaatccat tttactccgc tttcatcacc ccgaatgaca ttgccagatg tgttgctaga
    10681 aataattgca tttctagaca cagcatattc tgcaatcatt tttgcaatga agtttttatc
    10741 cttttgatcc tccaacacag catcatcatg tattaatctt ttaattcttc tacaaggctt
    10801 actgtcttta atataggcta ttgtttcatc tcttgtgaga gctttattac cattatcatt
    10861 taagttcggt aatacaacgt cttcccaata actttggaca tcttcattat cgtagtcaat
    10921 gatcaagctg ttgatatcca gagcaccttt gagagtcgat gatatctgca tccaaaccgt
    10981 ttctaaattc tctctagtat atattacaat catatttaaa tgatcggagt ctttcaaatc
    11041 ttgtaatagt ttaagtgttt tatcaggtgc attattatca agatgataat ctacaataat
    11101 aagatctgat tttctaatcc gatccacatc gaaattaaca gaaccattgt caacatcaca
    11161 aatcatattt ttagattgaa aaaagctctc aagagtagcg gctcgtttag atgagtcaat
    11221 tttgttgtag tctaaatcaa cttcgttatt caacgccctg attgattcag aa
//
LOCUS       DAHBHC010000120        10924 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHBHC010000120 DAHBHC010000000
VERSION     DAHBHC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.121"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(51..245)
                     /locus_tag="M2A35_004511"
     CDS             complement(51..245)
                     /locus_tag="M2A35_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HCB7200722.1"
                     /translation="MSTVIKRDKKQKGTGKAPPFHMRIVPELKEQFDNEASNDGVSLA
                     NWLKELGRAELKRRGIEPKG"
     gene            256..588
                     /locus_tag="M2A35_004512"
     CDS             256..588
                     /locus_tag="M2A35_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCB7200723.1"
                     /translation="MALCFGSLGVHLNTCTSKNATPRSGGTHAGRLTTNDNDSIEVAM
                     LNHTTHTQGRDSHNLNKYIWRFIALSTAQPRVIHIVATSEQEARQQSPAGCVMVFAAR
                     IRQGGCHA"
     gene            581..901
                     /locus_tag="M2A35_004513"
     CDS             581..901
                     /locus_tag="M2A35_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200724.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMGIASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATGALNAIKKANTD
                     SNRG"
     gene            908..1207
                     /locus_tag="M2A35_004514"
     CDS             908..1207
                     /locus_tag="M2A35_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCB7200725.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPTSRNNHVSRVDVDKKLQELKNMIAAWREQK"
     gene            1204..3021
                     /locus_tag="M2A35_004515"
     CDS             1204..3021
                     /locus_tag="M2A35_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCB7200726.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLDELDNLQIVPDGRYRVRFYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            3309..3554
                     /locus_tag="M2A35_004516"
     CDS             3309..3554
                     /locus_tag="M2A35_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200727.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFAALTEREIAEAEQVTTINVKTGEQTA"
     gene            3551..3994
                     /locus_tag="M2A35_004517"
     CDS             3551..3994
                     /locus_tag="M2A35_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7200728.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMASVAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALARHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTVRPGGKKGQQGQATDALNRAKQYAGHGVPDDNNAGDFDDDLPF"
     gene            4051..4575
                     /locus_tag="M2A35_004518"
     CDS             4051..4575
                     /locus_tag="M2A35_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HCB7200729.1"
                     /translation="MITLTLNHKSGDMKSRSGKPDKTTSAHKVMPGNQKKAQQHNKQK
                     STEATPWRHMTRRQRKNRRRVNRLIELWPDLFNREAPKPLKVGIFDDMMQDIATRGMK
                     FGLGTLRAALASYAQCPRYYRALVAGGARYDLKGQPCGEVTPSEQQDAEIRLMALNER
                     CKRQRQAAKEKPAA"
     gene            4572..4844
                     /locus_tag="M2A35_004519"
     CDS             4572..4844
                     /locus_tag="M2A35_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7200730.1"
                     /translation="MIHDSKAEELEAKGLYRRAAARWAEVMRLVNTDKEREQAAKRRA
                     ECIRKAARPPVKLDQYGDAKAAISRTQKEMGLHQPNGSAFRKWTGD"
     gene            5083..5256
                     /locus_tag="M2A35_004520"
     CDS             5083..5256
                     /locus_tag="M2A35_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200731.1"
                     /translation="MKAKNVRAVLLAREQIDRLERIRNDEAARSAYGIRPTIHEVARK
                     LIDEALNKGGRNA"
     gene            5249..6112
                     /locus_tag="M2A35_004521"
     CDS             5249..6112
                     /locus_tag="M2A35_004521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200732.1"
                     /translation="MPNRLLQYLGAGRISHLLDGGGKSEKKSKNASSGPSTAIRKQVS
                     DRNNAEATDKKENNVKMSFLHLNKGRNVGADNSGDTVNQPDVPDISEVKGAESLFRSN
                     EPEPLPKKHTREHNVSVSEEAKSNPVLALRLLRTTDLKSSAIIKKLREEPEAITAFTE
                     RHMTENSPEWEFVSDEDKARQAMIYSMVNDDSNGYSSGKTAALKVLDGMRDNYITQSE
                     FDLSLALYRENNPESRRIEAKKLQEEYARKAACEHVRYHQELKKIGQQVSPDDLDEDK
                     VYEAMKQRYAI"
     gene            6109..6771
                     /locus_tag="M2A35_004522"
     CDS             6109..6771
                     /locus_tag="M2A35_004522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200733.1"
                     /translation="MISSQRPDEQSPFAIASPPYSGGPLSGWQYHSVAQDDEHEGGLA
                     SVRKSNPAHCIATHPKARHNPVLAFHLIQSGANFAYADKQLNNSPRKLSGFTDRFMER
                     RVSGWELFSDEERNLAAFARSTSFNDDNGYMASMRAAERALQRYVIPLTLNEMVRKAK
                     ALYDDQHQAQIDAGHQRLKQMFENMGYTWTPPEDQKEPDGEVNRDPQYTGVYFRDMST
                     GI"
     gene            6768..7022
                     /locus_tag="M2A35_004523"
     CDS             6768..7022
                     /locus_tag="M2A35_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200734.1"
                     /translation="MSRYRKNKVVASGLAESWMLTAKKVTERVFGEGYAKAHPELITA
                     FMATAAANFATLNGREAAEAAELVSINVKCDNGTLTDERC"
     gene            7260..7409
                     /locus_tag="M2A35_004524"
     CDS             7260..7409
                     /locus_tag="M2A35_004524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200735.1"
                     /translation="MRKESEQQHSHIHEMIRKHIAELEKRTMAEFERCLKESAASSFQ
                     VNRHA"
     gene            7402..7782
                     /locus_tag="M2A35_004525"
     CDS             7402..7782
                     /locus_tag="M2A35_004525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200736.1"
                     /translation="MRDDVMQARAVQVLADVLASGIPPVEESRRKREHWAAELAHTEL
                     LVRQKKLITYAEWIDATATSYIAFRNRMMAIPAEHAPRLYNIAQSGDRKAFIDALTEL
                     TEEAMSNIDTPERIREAITQDKRQ"
     gene            7779..7967
                     /locus_tag="M2A35_004526"
     CDS             7779..7967
                     /locus_tag="M2A35_004526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7200737.1"
                     /translation="MMMSIADYARHAGVTRTTVYKWLKAEPSPHAKTVKSALKRPTTG
                     GAETSIMATSEEPTPAKH"
     gene            7988..8311
                     /locus_tag="M2A35_004527"
     CDS             7988..8311
                     /locus_tag="M2A35_004527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200738.1"
                     /translation="MQAVRKATEDSDHYRTLEESRALRECCAAEMMRLECRDFEGKFI
                     SPDYMIERMTSERDGIHNTVMEELNKHTSRLYRLAGKVSIIEFAEELRCVIYDALYRV
                     AYRER"
     gene            8858..9142
                     /locus_tag="M2A35_004528"
     CDS             8858..9142
                     /locus_tag="M2A35_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200739.1"
                     /translation="MTEAEMLKMIRRVAGIRPPAGKQEATQPDSVIAENYARVVAEVM
                     RRDGIELNGVDMRDIRTRVLELLSYRRRVQMYRESEKKTYQWKKPERLRR"
     gene            complement(9459..9728)
                     /locus_tag="M2A35_004529"
                     /pseudo
     CDS             complement(9459..9728)
                     /locus_tag="M2A35_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10045..10416)
                     /locus_tag="M2A35_004530"
     CDS             complement(10045..10416)
                     /locus_tag="M2A35_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200740.1"
                     /translation="MRLTSRKKEILSYYRPDHREWVTTEIGPPPFDVSGVAYLLHGMD
                     AFNKRHHLESTRRTLEAMVRDGVLERVNVYERRQDRTQTGDGAGVRCIVSRYGLPGQC
                     VVIRDNDGADNAINGECVRTA"
     gene            10530..10826
                     /locus_tag="M2A35_004531"
     CDS             10530..10826
                     /locus_tag="M2A35_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7200741.1"
                     /translation="MVHDDKAEELEAKGLWRRAANRWGELLKQSASDEARQYVTERRT
                     ACIRRATMPREPQRDQICAIRTAATKTLAGMGIDLKKEDPLRGTLYGHTGRDKK"
BASE COUNT         3122 a         2541 c         2925 g         2336 t
ORIGIN      
        1 ttattctctg gtagagaaag tttgttatcg gtctgctttt cagtcataca tcaacctttg
       61 ggttctatac cgcgccgctt tagttcagcg cgccccaact ctttaagcca gttggcaaga
      121 cttactccat cgttactggc ttcgttatca aactgctcct tcaattcagg gactatacgc
      181 atatggaatg gtggggcctt tcctgtgcct ttttgttttt tatcgcgctt aataactgtt
      241 gacatgtgta cacctatggc gttatgtttt ggttcgctag gtgtacacct taacacgtgt
      301 acatcaaaaa acgcaacgcc ccgcagtggt ggcacacatg cagggcgtct aaccaccaac
      361 gataacgata gtatcgaggt agctatgtta aatcatacca cacacacgca agggcgggac
      421 tcgcacaacc tgaataaata catctggcgt tttatcgccc tgagtacggc acaaccgcgc
      481 gtgattcaca tcgtggccac cagcgaacag gaagcacgcc agcaatcccc ggctggctgc
      541 gtgatggtat tcgccgcccg tattcgtcag ggggggtgcc atgcctgata tgtcaaatta
      601 ccagtacctg attaatccgc attttaactg tgagcatgat attgctaaaa aggtttattc
      661 cgctgcggat ggggctactg acaatatatc aatgggtatt gcgtcaattg gtagcctgat
      721 gtggcatgcg tcagaaaatg aggactatga cgaaaaggct atgcgcattg atatgggtaa
      781 tatcggtttg ttactggcaa tgcttgggca gtttgatatt tcgttacggt gcaccattga
      841 aaatgccaca ggtgcattaa atgccataaa gaaagcgaat actgattcaa atcggggata
      901 aataatcgtg agaacatatt tatctggctt gactgccagt ggttatgcac accccaaaat
      961 tatccccggc gctatttatc tggataagaa cggtaacaga gtaacggtaa aagaactgat
     1021 gtttgaccgt gtgtatttta ttcgtgatgg ctattcattt catagttcgc tgaacgtgga
     1081 gatctttatt agcagattca ggcgggaaat cccgacttcc agaaataacc atgtgtcacg
     1141 tgtggatgtg gataaaaaac tacaggaact gaaaaacatg attgccgcgt ggagagagca
     1201 gaaatgaaaa aagcgccaaa tttaaaacac cagccgcgtg acaaaatgac ggaagtcatc
     1261 atttttgcgg gtagtgatgc gtgggcacat gcgaagcagt ggcaggaaca ggacgggcga
     1321 ctggctggcg ataacgtgcc acctgtctgg cttggagagc aacaacttga cgaactggac
     1381 aacctgcaaa tcgtaccaga cggacgctat cgcgtgcgtt tctatcaggc ggggttattg
     1441 cgtccgggac ttgttaatac catcgggcag aaactggcag cggcaggtgt cagggatgct
     1501 gattattacc ctgaaggaat gcacagccag aaacgggaga actggcgcga atatctggaa
     1561 cgtgagcggg gagagctggc ggaaaagaaa aaggtagttg aactgcctgt aaagaaaaaa
     1621 gagcgggtaa aagacgataa cgcttcatca ctggcgctta accagatggg agcaagtcaa
     1681 cgcggcgaag ttctcctggc acattatggc ggtgaactgg cgattcatgc cgactctgac
     1741 actgttcacc attacaacgg cgttgtatgg gagccggttc aggataaaga gttacagcgt
     1801 gctatggcgc agattttcat tgatgcggag atcagctatt cgcagaacgc cattaaatcg
     1861 gcggtcgata ccatgaagtt aagtttgcct gtaatgggga atacagcccg taacctgatt
     1921 ggattcagta acggggtatt tgataccaga acaggtaatt ttcgggagca taacaaaaac
     1981 gactggttgt taattgccag tgaattacct ttcagcccac cagcagaggg ggaaacgctg
     2041 gcaacacatg cgccgaattt ctggaagtgg ttacgccgtt cggtggctga gaatgaccgc
     2101 aaggcggatc gcgtactggc tgcattattc atggtgctgg cgaaccggta cgactggcag
     2161 ttattcattg aggtaacagg tccaggggga agtggtaaaa gcgtgatggc ggagatttgc
     2221 accatgctgg cgggtaaggc caacacagta tcggcaagca tgaaggcgct ggaagatgca
     2281 agggaacgcg cgttagtggt tggcttttcg ctgattatca tgccggatat gacccgctac
     2341 gctggtgatg gggcagggat taaggccatt acaggcggtg acaaggtggc aattgacccg
     2401 aaacacaaag ccccctactc aacgcgtatt ccggcagtag tgctggcggt taacaataac
     2461 gccatgtcat tcagtgaccg cagcgggggg atctcacgtc gtcgggtgat attcaatttt
     2521 tcggaagttg taccggagaa cgaacgcgat ccaatgctgg cggaaaaaat agaaggtgag
     2581 ctggcggtag tgattcgcca tctgcttaca cggtttgctg accaggacga agccagacgc
     2641 ctgttatatg agcagcagaa atctgaagaa gcactggcga taaagcgaga gggggattcg
     2701 ctggtggact tctgcggcta tctcatggcg tcggtaatgt gtgatggcct gttagtgggt
     2761 aatgctgaaa ttgtgccatt cagcccacgc aggtatctct atcatgccta tctggcttat
     2821 atgagggcac atgggtttgg taaacctgta acactgacgc gcttcggtaa agatatgccg
     2881 ggggcaatgg cggaatatgg cagggagtat atgaaacgga aaacgaagca cggtttgcgt
     2941 tcaaacgtga cactgacgga ggaatcagaa gactggatgc catcatgtgt atcggtcact
     3001 aatgacgata gcaaaaatta aacttatgga ataactgttc accactgttc accctgtcat
     3061 aaatatcttt tatatcagta tattataggg tgaacagtta tttatgaact gttcaccaaa
     3121 ctattcactg ttcacttttt tgattgttta ttgagcttca agggtgaaca gtggtgaaca
     3181 gttggtgaat agtttttgtg aaactgttca ccccttaaca ttatgaatta aaagagaaaa
     3241 tatcaaaagg tgaacaggtg aagggttaaa atgcaaaaat tttaatttac tgctgtgaga
     3301 taaagcctat gacagcgaag cacacaaaaa aatcacaatc gcacgccctt gatttgacgg
     3361 aacactggtt aagggtgtcg ataaaaatca tcgaccgcaa cgccggggaa ggatatgcga
     3421 aagcacatcc cgaactgatt agcgccttca tgacaacggc agctgcaaac tttgccgcgt
     3481 tgacagaacg ggagattgcc gaagcggaac aggtaacaac catcaacgtt aaaaccggag
     3541 agcagacagc atgacagcac agatagcggc ttacgggcgg ctggtggctg acccgcagtt
     3601 aaagaccacc agcaagggta cacaaatggc gatggcaagt gtggcggtcc ccctgccgtg
     3661 cagccaggcc gatgacggaa cggcgacgat gtggttatcc gtcctggcat ttggcagaca
     3721 ggcagacgca ctggccagac accacaaagg cgaactggtg agcgtggcgg gtaacatgca
     3781 ggtaagccag tggacaggcc agaacggcga aacgcggcag ggctggcagg ttatcgcaga
     3841 cagcgtaatc agtgcccgaa ctgtgcgacc gggcggcaaa aaaggccagc aggggcaggc
     3901 tactgatgca ctgaacagag cgaaacagta tgcagggcat ggcgtacctg atgacaataa
     3961 cgccggtgat tttgatgatg acctgccctt ctgaggcagg tcatcacaca ggcagtaagc
     4021 aacaagaagt aagatgcaag agagaaaaaa atgataacac tgacactgaa ccacaaaagc
     4081 ggggacatga aaagccggag tggaaagccg gacaaaacca catcggcaca taaagtgatg
     4141 cccggtaatc agaagaaggc tcaacagcac aataaacaga agtccacaga agcgacaccg
     4201 tggcggcata tgaccagacg ccagcgcaaa aaccgccgac gggttaaccg cctgattgag
     4261 ctgtggcctg acctgttcaa cagggaagca ccgaagccgc tgaaggtggg gatattcgac
     4321 gacatgatgc aggatatcgc taccaggggg atgaagttcg gactcggtac attacgtgcg
     4381 gcgctggcat cttatgcgca gtgtccgcgc tattaccgtg ccctggttgc tggtggagca
     4441 cgttacgacc tgaaaggcca gccttgtggc gaggtgacac catcggaaca gcaggacgca
     4501 gaaatacgac tgatggcgct gaatgaaagg tgcaaacgtc agcgccaggc agcaaaggag
     4561 aaaccagccg catgattcat gacagtaagg cggaagagct ggaggctaag ggactgtacc
     4621 ggagagcagc ggcccgatgg gctgaggtga tgcggctggt gaacactgat aaggaacgag
     4681 agcaggcggc aaaacgtcgc gcggaatgta tccgtaaggc agcacgccca cccgtgaagc
     4741 tggatcagta cggtgatgca aaagccgcaa taagccggac gcaaaaggaa atgggactgc
     4801 atcagcccaa cggcagcgca ttcaggaaat ggacgggcga ctgatacccc cactccgtca
     4861 ggcatatcac gacaaagaaa caaagctaca gggccggatc tgacatctgg ccttttttat
     4921 tgcaaaaaaa accgccagag agctggcggt cgttaaagga tgacggttgc aaaaacactg
     4981 aaaccccgtt cctgattaat attacattcg tgtttcatta atcgcaataa tgagtgctat
     5041 cattgcaata tgtgaaacaa taaacactat cggggttatc acatgaaagc aaaaaacgta
     5101 agggccgtac ttctggcccg tgaacagata gatcgccttg aacggatccg caacgacgaa
     5161 gcggcacgtt ctgcctacgg tatcaggcca accattcatg aagtggcaag aaaactcatt
     5221 gatgaggcgc tgaataaagg ggggcgcaat gcctaatcgt ttgttgcaat atctgggggc
     5281 cgggagaatt tcgcaccttc tagatggcgg gggaaaatcc gaaaaaaaat caaaaaacgc
     5341 ctcatcgggg ccatcaacgg caatccgcaa gcaagtatca gaccgcaata atgcagaagc
     5401 tacagacaaa aaggaaaaca acgtgaaaat gtctttttta catctcaaca agggccgaaa
     5461 tgttggtgcg gacaattctg gcgataccgt taatcagcct gatgttcctg acatcagcga
     5521 agtaaagggc gctgaatccc tctttcgcag caacgagcct gaaccgttac ccaaaaaaca
     5581 cacacgggaa cataacgttt ccgtttcgga ggaagcaaaa agtaatccgg ttcttgctct
     5641 ccgacttctg cggacaaccg acctgaaatc atccgccatc atcaaaaaac tacgcgaaga
     5701 acccgaggct attaccgctt tcactgaacg gcacatgaca gagaattcac cggaatggga
     5761 gttcgtcagc gatgaagaca aagcccgtca ggcgatgatt tattcgatgg ttaacgatga
     5821 cagcaacggc tatagttccg gcaagaccgc agcgctgaaa gtgctggatg gaatgcgtga
     5881 caactacata acacagtctg agtttgatct ctcgctggcg ctttacaggg aaaacaaccc
     5941 tgaaagccgt cgtatagagg ccaaaaaact acaggaggaa tatgcccgaa aagctgcctg
     6001 cgaacacgtc agataccacc aggaactgaa aaaaatcgga caacaggttt caccggatga
     6061 cctggatgaa gacaaggttt acgaagccat gaagcaaagg tacgcaatat gatatccagc
     6121 cagcgcccag atgagcaaag cccttttgcc attgcgtcgc cgccttattc aggcggcccc
     6181 ctcagtggct ggcaatacca ttcagtcgca caggatgacg agcatgaagg cgggctggca
     6241 tccgtcagaa aatcaaatcc ggcgcactgc atagccacgc atccgaaggc aaggcataac
     6301 cctgtcctgg cctttcatct cattcagtcg ggggctaatt ttgcttatgc agacaaacaa
     6361 ctcaataaca gtccccgcaa actttccggc tttacggatc gttttatgga acgacgggtg
     6421 agcggctggg aacttttttc agatgaagaa cgcaaccttg cagcctttgc ccgttcaaca
     6481 tcattcaacg acgataacgg ctatatggcc tccatgcgtg ccgcggaaag ggcactacag
     6541 cgttatgtta tcccgttaac gcttaacgag atggtcagga aagccaaagc cctttatgac
     6601 gatcagcatc aggcacagat tgacgcaggc caccagagac tgaaacagat gtttgagaac
     6661 atgggatata cctggacacc accggaagat cagaaagaac ctgacggtga agtaaacaga
     6721 gatccccagt atacaggtgt ttattttcgg gatatgagta caggaatatg agcagataca
     6781 gaaaaaacaa agttgttgcc tccggccttg ctgaaagctg gatgcttacg gcgaaaaaag
     6841 tcaccgagcg cgtgtttggt gaagggtatg cgaaagcaca tcccgaactg ataaccgcat
     6901 tcatggcaac ggcggcggca aactttgcca cactgaatgg tcgtgaggca gccgaagcgg
     6961 cggagcttgt cagcattaac gtgaagtgtg acaacggcac gctgactgat gaacggtgtt
     7021 gagttcgcag ggaaggataa tataaccccc atggggggta acgggtcctt cctgagcgag
     7081 ttattgcacg ggcattgcgc gagcgcggtg tttcactagt gtaaaccttc cgaatttgat
     7141 ttacacttta cacgtccgaa actcccccgc aagccgcgcc atctctggaa aaattaaatt
     7201 ccacgcgatg aatttgattt acacttttga tttacacact ggcaggaggt ttacacttta
     7261 tgcgtaaaga atcagagcag cagcattccc acatacacga gatgatccgg aagcacattg
     7321 cagagctgga gaagcggacg atggcggagt ttgaacgctg cctgaaagag agtgcggctt
     7381 catcgtttca ggttaatcgc catgcgtgat gatgtgatgc aggcacgggc ggtgcaggtt
     7441 ctcgccgacg tgctcgcgtc cggcattccc cccgtggagg agtcccgcag gaagcgggaa
     7501 cactgggcgg cagaacttgc gcatactgaa ttactggtaa ggcaaaagaa actcatcact
     7561 tatgctgaat ggattgatgc aacagcaaca tcatacattg cattcagaaa caggatgatg
     7621 gccattccgg cagagcacgc cccccgcctg tacaacatag cgcagagcgg agatcgtaaa
     7681 gcctttatcg atgccctgac agagcttacc gaggaggcga tgagcaatat cgacacccct
     7741 gagcgcatca gggaagccat cacgcaggat aaacgacaat gatgatgagc attgccgact
     7801 atgcccgcca tgcaggcgtt acccgcacca ctgtctacaa atggcttaag gccgaacctt
     7861 ccccccacgc aaagacggta aaatctgcgt taaagaggcc gacgactggc ggcgcagaaa
     7921 catcgatcat ggcgaccagc gaagaaccaa cgcccgcaaa gcattaaacg cacgtgaaga
     7981 aaaggttatg caggctgtca ggaaggcaac ggaagacagc gaccattacc gtacactgga
     8041 ggaatcaagg gcactgcgtg agtgttgtgc ggcggagatg atgcgactgg aatgcaggga
     8101 tttcgaaggt aagtttatct cccctgatta catgattgag cgcatgacca gcgagcgcga
     8161 cggtatacac aatacagtaa tggaagaact caacaaacac acatcacggc tttaccgcct
     8221 ggctggcaag gtgtcgatta ttgagtttgc ggaagaactg aggtgtgtca tttacgatgc
     8281 gctgtatcgt gttgcttatc gtgaaagata aggccagtaa gatttttaaa gatgtaacgc
     8341 cctgaaaaag cggcccatga ggccgcaaat gatcctgagc tttatacggg ttatacagcg
     8401 tataaccgta gcaatggtta cctggctgag cattgtctga caatacggag gcattgtttt
     8461 tttgtgaatt aagtcacact tttgattacc ctgtacactc cgtacgtatt acgtacgcac
     8521 ctacaccaga gcaaaaagat tgaggctggc aaaagcatca cggaacggga agagcaccat
     8581 gccaacggcc cggcgaaatg gcgcaaatcc cgcgcaaaaa aacacgaatc cgttatccac
     8641 ttacatgatg gctttatgcg ttctgcgttg tgctggtggg ctgcacaatc attatgatga
     8701 tgcagctatt accctgccac acaaaagggg cggtgattat cgcctcccct tcagagcggc
     8761 ccgccaccag aacaaacacc acagacggac ttcgccttcc ttgtgttaac caccacaatt
     8821 acaacattgt ttccccatca ccacggaggc tgtgacaatg accgaagccg aaatgctcaa
     8881 aatgattcgc cgtgttgccg gaatcagacc accagcaggc aaacaggagg ccacgcagcc
     8941 ggacagcgtg atagccgaaa attacgcgcg tgtggtggcg gaggtgatgc gccgtgacgg
     9001 tattgagctt aacggcgtgg atatgcgcga catacgaacc agagttctgg agttgttgtc
     9061 atatcgtcgc cgcgtgcaga tgtaccggga gagcgagaaa aagacttacc agtggaagaa
     9121 gccggaacgg ttacggcggt aagttattgg ttaaatggct cctcaatttt gaggatggta
     9181 gctggtggcg gtaagtcgtt gattaagagg cggctcaaaa ttgagccggg gtgctggtgt
     9241 cggtagcccc atgaaagggg ctttgaaggg gatggttata atgaccatcc cttttgtgtg
     9301 gcaaggtggt agctccatgg tcaaaatgac cacggagggg agaaccgact atctcaaaga
     9361 tagtcagacc tacgaaattt tcgtatcacc tgatatcgtg acagagagtg acagaccgtg
     9421 acaaacagac ggccttatct ggttactgtc ggatggttat tggcaatggc cctgataact
     9481 gccgtttctc tttgtggctt tggtgggatg ttcggctttc cggcaataag cgacgcagcg
     9541 gctttctcca ttcgctccgc cagctcatgt aaccgtgaat caacagaaat ttccggctgt
     9601 ccgagcttca tacgaacagc atcaacaagc caggcagttt tgttcccccc ggaagcgata
     9661 acagagctgt taaactcatc cagtaattca ggtgggatgc ggaacgcgac cagaccagat
     9721 ttgctcattg taaacgacca tatcagcgag aacatctata cagcatacca ctgtataacg
     9781 ctgttatacg ctcaaacatc aaccagaaaa taacgagtca gtcacaaagc cagtgattca
     9841 ggccacacat ggcagcagca ccataacgcc cacaaatacc accagcgcaa cgctgagcaa
     9901 ataaagctgc gaacacgggg aaaccataaa acaacgccag ggacctgatt gtggcgctat
     9961 atgttggtgt ttttgacggg gagtatttat tgcctcccct ttcgttcgct gtgtggttac
    10021 ggcatattca taacgcccgt acagctatgc cgttctgaca cattcaccgt taattgcgtt
    10081 atctgccccg tcattgtcac ggataaccac acactgtccc ggcagaccat aacgggaaac
    10141 gatacagcgc acaccagcac catctcccgt ttgtgttctg tcctgtcgcc tttcgtaaac
    10201 attcacccgc tcaagcaccc cgtcgcgaac cattgcctca agcgttcgtc tggttgattc
    10261 aagatgatgc cgtttattga atgcatccat gccgtgaagc aggtaagcca caccagatac
    10321 atcaaatggc ggcggaccga tttcggttgt tacccattcg cggtgatccg gacggtagta
    10381 gctgagaatt tcttttttgc gactggtaag cctcatctgg tcagtcctta ttgtaggaga
    10441 gatgtctcat acaataagct atatggcaga ttttaaaaga acagaaatat gccagttcag
    10501 atttaacaga tatcaacgga gtcaggctta tggtgcatga tgacaaagct gaggagctgg
    10561 aagcgaaggg gttatggcga cgcgcggcca accgctgggg cgagttactg aaacaatctg
    10621 cgagtgatga agcgcgtcag tacgtgacgg aaagacgaac ggcatgtatc cgaagggcaa
    10681 ccatgcccag agaaccgcaa agggatcaga tttgtgccat aagaaccgct gcaacaaaaa
    10741 cactggctgg catgggcata gaccttaaaa aagaagatcc actgcgtggt acgttatacg
    10801 ggcacacagg gcgcgataaa aagtgaactg atggagcaag gaaacaaaaa aaggctcgcc
    10861 gtaacgagcc gtaacctctg gggaaagttg gcaatatgaa gataataaat gatgatggag
    10921 aaaa
//
LOCUS       DAHBHC010000121        10693 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHBHC010000121 DAHBHC010000000
VERSION     DAHBHC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.122"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..561)
                     /locus_tag="M2A35_004532"
     CDS             complement(<1..561)
                     /locus_tag="M2A35_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCB7200742.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHD"
     gene            complement(739..1650)
                     /gene="gsiD"
                     /locus_tag="M2A35_004533"
     CDS             complement(739..1650)
                     /gene="gsiD"
                     /locus_tag="M2A35_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCB7200743.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1653..2573)
                     /gene="gsiC"
                     /locus_tag="M2A35_004534"
     CDS             complement(1653..2573)
                     /gene="gsiC"
                     /locus_tag="M2A35_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCB7200744.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2591..4129)
                     /gene="gsiB"
                     /locus_tag="M2A35_004535"
     CDS             complement(2591..4129)
                     /gene="gsiB"
                     /locus_tag="M2A35_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCB7200745.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4149..6020)
                     /gene="gsiA"
                     /locus_tag="M2A35_004536"
     CDS             complement(4149..6020)
                     /gene="gsiA"
                     /locus_tag="M2A35_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCB7200746.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPETQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAACVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6007..6972)
                     /gene="iaaA"
                     /locus_tag="M2A35_004537"
     CDS             complement(6007..6972)
                     /gene="iaaA"
                     /locus_tag="M2A35_004537"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCB7200747.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7176..8411
                     /gene="moeA"
                     /locus_tag="M2A35_004538"
     CDS             7176..8411
                     /gene="moeA"
                     /locus_tag="M2A35_004538"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCB7200748.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASNV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8411..9160
                     /gene="moeB"
                     /locus_tag="M2A35_004539"
     CDS             8411..9160
                     /gene="moeB"
                     /locus_tag="M2A35_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCB7200749.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9236..9898)
                     /gene="fsa"
                     /locus_tag="M2A35_004540"
     CDS             complement(9236..9898)
                     /gene="fsa"
                     /locus_tag="M2A35_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCB7200750.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            10029..>10693
                     /locus_tag="M2A35_004541"
     CDS             10029..>10693
                     /locus_tag="M2A35_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCB7200751.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPL"
BASE COUNT         2597 a         2994 c         2650 g         2452 t
ORIGIN      
        1 atcgtgatct ttgccgttga tacgtagcgt aaaagagata cattttttac ccggacgatc
       61 aaagcaattg tatgccggat tccagctgtt gttagagtca tctgaagcca ccgaccacgg
      121 tcgtttcgat gggttaaaat cggcaaaaat aacccgccct gactcgtcga caagcggtcc
      181 atttacttgt tcctgatgat aaacaacgat tttatcattc gccctccgcg aaaagagata
      241 atatttatta tcatcaagta ccagtcccac cgagtctaaa aatggcatca tttccatttt
      301 cagaatcacg gcgcgttgaa tctcctctgt attttttaat aaatacagtg agtcctttaa
      361 tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc
      421 aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc agttgatagt taatgatgaa
      481 aaaaagacca ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat
      541 cttttcgtat aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc
      601 cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc
      661 agcagaaagt tacaacactt gcttagggca ataatttcct gtgaggtgat taccctttca
      721 cgcaatattc aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc
      781 gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac
      841 atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt
      901 cggcggctgc gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc
      961 aatgcgcatg gtgaaaaaca ccacgataga cgagacggtc ccaggcaaga tatgacgcaa
     1021 taaaatggtc atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt
     1081 caacaccagc gtgttgccgc gcaccagtcg ggcaaacgcg gggatggaaa aaatggcgac
     1141 tgcgataatc acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa
     1201 aatacccggg aaggcaaaca gcacatcgca aatgcgcatg atcaaccgat cccaccagcc
     1261 ttcataatat ccagccaaca agcccagcaa cgtcccgatc gccgcaccga taaacacggc
     1321 aaacacgccc gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg
     1381 ccccagtgaa tcgacgccaa accagtgctg caaagaaggt ccgttattca ggttgtcata
     1441 atcaaaataa ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat
     1501 caataaaata acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca
     1561 gaattcatgc cacggtgtac gcacctggtc aggtttgacc agtggcatgg cgtttaacac
     1621 cgcctgacgt cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg
     1681 gcggcgtaaa gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga
     1741 atttccgcct gaatcaccgg gtaatcgcgc atttctacgg agtcaacgag taagcgtcca
     1801 agtccgggcc agttgaaaac tttctccacc acgatagatc caccgagcaa aaagccaaac
     1861 tgtaagccca tcatggtcac taccgggatc atcgcgttgc gtagcccgtg tttgaggaca
     1921 acccaggttt cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaacg
     1981 tcgacaaatg acgcacgggt aaagcgcgcc atcacggcgg ccactgccgc gccgagcgtc
     2041 agggagggta aaatgtagtg ctgccaactg tctgctccca cagtaggcag ccagcccagt
     2101 tcaacggaga atacctgaat taaaagcatc cccagagcaa atgccggaaa cgagatcccc
     2161 gacaccgcaa tggtcatact caatcgatcc ggccaacggt tacgccagac ggcggcgata
     2221 attcccgccg ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc
     2281 gttggcataa agcggctggc aatctcatcg gcaactggac gacgcgacac catggacagg
     2341 ccaaaatccc cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga
     2401 tccaacccca gctgctgacg caccagttct ataacctgcg catcagcttc gggcccggca
     2461 atcaatcgcg ccggatcgcc gggcagcata tggacaaata aaaacaccag caccgagacg
     2521 ataaacagcg tcggaatcaa ccccagtaat cgtttgataa cgtaattaag cattccactc
     2581 cctgcgttgc ttattgcaaa tccgcatctt caaagctgaa gccggtgtct ggcatgatcc
     2641 aaaaaccggt caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc
     2701 acggcgactc ttgccagatg atatcctgcg ccgccttata taaacgggtc ttttccgccg
     2761 gatcattagt tttcagtgcc tgagccagga agtcatccac ctgtttattg ctgtaaaacg
     2821 cggtattaaa cagcgtcggt ggccagtttt gcgaggcaaa caacggcgat agtgcccagt
     2881 cggcttcgcc ggttgaagcc gaccagccag tgtagaacat ccgcacgccg ctctcttttt
     2941 gccctttacc ttcaacttct gccgcccgct gtccggcatc catcgcagtc acctgggctt
     3001 taatcccgac ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt
     3061 ggttatgtga cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta
     3121 attcgcgcgc tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac
     3181 ttggcggtac cacaccagta gctggcgttg catagcccgc aaaggcaact ttcaccagcg
     3241 ccggacggtt aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt
     3301 gcgtcacgtt catactgata taacgctgca taattgacgg actggccatc aactcgatat
     3361 ttttgttttt ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt
     3421 caccggtttg cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc
     3481 tgtccagttt gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat
     3541 cggtctgatt ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct
     3601 ccttgccata tttttccagc gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa
     3661 tattaataaa cgctgagaac ggctgtttga gagtaatctt tactgttgtt ggatcgatcg
     3721 cctccgtttt ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc
     3781 tggcccggtc cagattcgct tttaccgccg ctgcgttgaa atcggtgcca tcctggaatt
     3841 taattccttc ccgcaatttc acggtgtatg taaggccgtc atcggaaacg gtataactct
     3901 ccgccagcac gtttttcagt ttcatctctt tatccagacc aaacagcccc tggtaaaacg
     3961 atttcgctac ggcctgagat aacgtgtcat ttgcgtcata cggatcgagc gtggtgaagt
     4021 tcgatcctac cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg
     4081 tcgcaatgcc caacgccact aacccactac ggtgtacagc tcttgccatt ttattctccg
     4141 cctgaatgtt atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc
     4201 ggcccgacgc attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgctg
     4261 ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga
     4321 actgccgcca gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt
     4381 ggaccaattt caacaatttg tccgagatac atcaccgcca cacgatgact aatccgctct
     4441 accacggcca tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg
     4501 agcaacaagt tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg
     4561 gcaatgatca cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga
     4621 ccgccggaaa actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc
     4681 agcaaccacg caacgcatgc agccgcgtct ttacctggca ataaaccgtg tacacgcagc
     4741 ggttcgataa tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga
     4801 aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc cgggtgacaa ggtatcgatt
     4861 ctctgaccgt taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc
     4921 ccggtagtgg atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag
     4981 agatcaaaac tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg
     5041 ctgcgcaaag ggaaacgggt gacaagatta cgcactcgta aaacaggttc gccatccacc
     5101 accgttttct gctcgatggg gggggcctgt ttcgctggat gttcaagcga tatcaacggg
     5161 aaacgtcggg gataatctaa ccctttcatc gcaccaagtt gcggaacagc agctaacagc
     5221 gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc
     5281 gcctcgccct gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg
     5341 tgagtgataa agataacgcc catcgacatc tctttttgca atactttgat taattgcagg
     5401 atctgcgcct gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc
     5461 gggcggcatg acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga
     5521 tgcggataac gtgaaagaat ggtttgtgtc tcaggaatgc gtacctgatc cagcatccgc
     5581 ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca
     5641 atctgttcac ccacagtaaa taccgggttc agcgatgtca tcggctcctg aaatatcatc
     5701 gccatatccg caccgcgaac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc
     5761 acttcgcgac tgcgccgctg caacagcatt ttatcgcact gtactaaacc gcccgcctgt
     5821 tccaacaggc gcatcaatgc caacgcagtc actgacttac cggagccgga ttcgccaaca
     5881 attgccagcg tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc
     5941 tgctggtcct gcataaaggc aatattcaga ttttcaaccg ccagcacatt accggcatca
     6001 agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt
     6061 atcgcctgcg tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt
     6121 cccttcatgg tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat
     6181 tactacccgc tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat
     6241 gtcatatgcc gccagcgcgc ggatgaagac ttcgcccgtg ccggtacaag aaaccgccac
     6301 actggcgtta ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg
     6361 taatttattg gtcattccgc ctgtggacgt ggctgccgcc aaattgccgt ctaaatccaa
     6421 cgccacggcc cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc
     6481 gaggacggtt gccccttcct tgcgcgctgc cagtagttgt tcataacgca aaggcgtgga
     6541 gaaaatctcc ggcgagacgc gctccatgcc acgagcaaac gcaaaatttt ctgccccttc
     6601 gccaatcatc atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt
     6661 acgcagatga ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca
     6721 ggcgtccagt tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag
     6781 tggacactct tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc
     6841 ttccagcatt ttctgcccgg tttcaacaat ggcagacaac gcctcaatgt agcgtaattc
     6901 ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac
     6961 tgctttgccc atagtattcg tccattaatg gtggctcgcc acttttgcta taaatatcag
     7021 aatttttatg aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag
     7081 gcaggagcca acgctacgcc cgaggtggca tacagtctgt ggcgtttttc tgacataata
     7141 ggcaaattcg attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt
     7201 cgctcgacac cgcgcttaat gagatgcttt ctcgcgtcac cccactgacc gcccaggaaa
     7261 cgctgccact ggtacagtgt tttggtcgta ttctggcgag caatgtcgtt tcgccgcttg
     7321 atgttccagg gtttgataac tccgcaatgg acggctacgc ggtgcgttta gccgatattg
     7381 cctccaggca accgctgccc gttgccggta aatcctttgc cggtcagccg taccatggtg
     7441 aatggcctgc gggcacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg
     7501 aagcggtggt gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg
     7561 aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg
     7621 ttttcccggc gggaactcgc ctgactaccg cagagctgcc agtaattgct tcactgggga
     7681 ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac
     7741 tccagttgcc cggtcagccg ctgggcgacg gccaaatcta cgacaccaac cgtctcgccg
     7801 tacacctgat gttagaacag ctgggttgcg aggtaatcaa cttagggatt atccgcgacg
     7861 atccccatgc cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtagtgatca
     7921 gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa aacgattctt gaagagctgg
     7981 gggagatcgc cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac
     8041 tcagcaatag ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct
     8101 atcaactggt acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg
     8161 cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc
     8221 agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc
     8281 agggttcaca tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg
     8341 atcgcggcaa tgtggacgtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg
     8401 gaggcctgta atggcggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat
     8461 tctgcgcggt tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctggtagt
     8521 cggcctcggt ggactcggct gcgcagcttc gcagtatctg gcaagcgccg gtgtcggcaa
     8581 cctgacgctg ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc agacgctgca
     8641 cagtgatgct acggtcgggc aaccgaaggt agaatccgcc cgtgacgcca tgacgcggat
     8701 caacccacat atcgcgatta cgccagtcaa tgcactgctt gatgacgctg aacttgctgc
     8761 gatgattgca aaacacgatc tggtactcga ctgtacggat aacgttgcgg tgcgtaatca
     8821 actgaacgct ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat
     8881 ggaagggcaa atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag
     8941 ccgtttgttt ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat
     9001 cggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa
     9061 accggccagc gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa
     9121 actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggccagat
     9181 aagacgcggc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
     9241 tcgacgttct gccaaacgct cccagccagt cctgttcaaa cttcgccaca gcggcttcca
     9301 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagcgtaatt gattcacatc
     9361 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
     9421 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
     9481 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
     9541 acagtagccc ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt
     9601 ttaacatctt aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg
     9661 caataataga acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta
     9721 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
     9781 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
     9841 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
     9901 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
     9961 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
    10021 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
    10081 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
    10141 gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
    10201 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
    10261 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa
    10321 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac
    10381 cgttttacga tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc
    10441 cggaaatggc gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag
    10501 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
    10561 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
    10621 ccgccagagt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
    10681 gcgtgccgct gat
//
LOCUS       DAHBHC010000122        10582 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHBHC010000122 DAHBHC010000000
VERSION     DAHBHC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.123"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(42..395)
                     /locus_tag="M2A35_004542"
     CDS             complement(42..395)
                     /locus_tag="M2A35_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCB7200752.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(533..2179)
                     /gene="groL"
                     /locus_tag="M2A35_004543"
     CDS             complement(533..2179)
                     /gene="groL"
                     /locus_tag="M2A35_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCB7200753.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2223..2516)
                     /locus_tag="M2A35_004544"
     CDS             complement(2223..2516)
                     /locus_tag="M2A35_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCB7200754.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2792..4048
                     /gene="yjeH"
                     /locus_tag="M2A35_004545"
     CDS             2792..4048
                     /gene="yjeH"
                     /locus_tag="M2A35_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCB7200755.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4064..4540)
                     /gene="fxsA"
                     /locus_tag="M2A35_004546"
     CDS             complement(4064..4540)
                     /gene="fxsA"
                     /locus_tag="M2A35_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCB7200756.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4877..6313
                     /gene="aspA"
                     /locus_tag="M2A35_004547"
     CDS             4877..6313
                     /gene="aspA"
                     /locus_tag="M2A35_004547"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCB7200757.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6431..7732
                     /gene="dcuA"
                     /locus_tag="M2A35_004548"
     CDS             6431..7732
                     /gene="dcuA"
                     /locus_tag="M2A35_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCB7200758.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7848..8186
                     /gene="cutA"
                     /locus_tag="M2A35_004549"
     CDS             7848..8186
                     /gene="cutA"
                     /locus_tag="M2A35_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCB7200759.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8162..9859
                     /gene="dsbD"
                     /locus_tag="M2A35_004550"
     CDS             8162..9859
                     /gene="dsbD"
                     /locus_tag="M2A35_004550"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCB7200760.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRDWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9896..10471
                     /locus_tag="M2A35_004551"
     CDS             9896..10471
                     /locus_tag="M2A35_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7200761.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2466 a         2782 c         2639 g         2695 t
ORIGIN      
        1 atcaggcaat gatacgtttg tcacccagcc tctggatcac atcagttcgg gcacttataa
       61 acctgaccga taatctggct atccgtcggg acaaaactgg acaacattcc ctgcgacggg
      121 ctgctgatgc cataaatcac gttaccgccc attgcagccg cctggttgcg cagatcgttt
      181 gctgcgccgc gcatagaacc gccctcttct ccgtgttgcc cggaaagcca gttgctttgc
      241 ttacctgtcg cagtaccaat cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt
      301 acactctgac cggcagcact caattcgttg ctggagctac aacccgccat cagtagcgcg
      361 gcaccgacaa tccctgctaa gtattttacg tgcatgttat tccccataat caatgagctg
      421 gacgccactc gcgtcgtccg tgtctgaatc ttatactaga aagatgacca aaagaaaaac
      481 ccccagacat ttctgcccgg gggtttgttt atttctgcga ggtgcagggc aattacatca
      541 tgccgcccat gccacccatg ccgcccatac cgccagcagc gcctaagtca gctgcatcat
      601 ttttcggcag gtcggtaacc atgcactcgg tggtgatcat caggccagcc acagaagccg
      661 cgtactgcag agcagaacgg gttactttgg ttgggtccag gatacccatg tcgatcatgt
      721 tgccgtattc ttcggttgct gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt
      781 tagcaacaac agacggttct tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca
      841 ttgcacgcag tgcaactttg atacccacgt tctggtcttc gttctgacca cgcaggtcag
      901 ccagtttaga cgctacgcgg atcagcgcaa caccaccacc agcaaccacg ccttcttcta
      961 ccgcagcacg ggtcgcgtgc agggcgtctt caacgcgtgc ttttttctct ttcatttcaa
     1021 cttcggtagc agcacctact ttgataactg caacgccgcc tgccagtttc gctacgcgtt
     1081 cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc aatctgctga cggatctgag
     1141 caacacggcc ctggattgca gcttcttcac ccacgccatc gatgatagtg gtggtgtctt
     1201 tgttgatcac aacacgttta gcctgaccca ggtcttccag ggttgctttt tccagctcca
     1261 taccgatctc ttcagagatt acggtaccgc cagtcagggt tgcgatatcc tgcagcatag
     1321 ctttacgacg atcgccgaag cccggagctt taaccgcagc aactttcacg atgccacgca
     1381 tggtgttaac aaccagagtt gccagcgctt cgccttcaac atcttcagcg atgatcagca
     1441 gcggtttgcc tgctttggca acagcttcca gaaccggcag catttcgcgg atgttagaga
     1501 ttttcttgtc agccagcagg atgaacgggc tttccagttc tactgcgcca gtttccggct
     1561 tgttgatgaa gtaaggagac aggtagccac ggtcgaactg cataccttca accacgtcca
     1621 gttcgtcctg cagaccggta ccgtcttcaa cggtgataac gccttcttta ccgactttgt
     1681 ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga gttagcggag atggtaccaa
     1741 cctgagcaat cgctttagag tcagagcacg gtacggacag cgctttcagt tcttcaactg
     1801 cagcggtaac ggctttgtcg ataccacgtt tcaggtccat cgggttcatg cccgcagcaa
     1861 cagctttcag accctcagtg atgatagcct gagccagtac agttgcagtg gtggtaccgt
     1921 cgcctgcagc gtcgttcgct ttagaggcaa cttctttcac catctgcgca cccatatttt
     1981 cgaacttgtc ttccagttcg atttcacgag caacggaaac accatctttg gtgatggtcg
     2041 gtgcaccgaa agatttatcc agaactacgt tacggccttt cggaccgagg gtaactttca
     2101 ctgcatctgc cagtacgttt acgccgcgca gcattttcac acgagcgtcg ttaccgaatt
     2161 ttacgtcttt agctgccatt atctttattc cttaaattcg tatgttcagt gtcgtgcgag
     2221 gattacgctt caacaattgc cagaatgtcg ctttcggaca tgatcaacac ttcttcattg
     2281 tcgatcttct cagatttcac accgtagcca tcgttgaaaa taacgatgtc gccaactttc
     2341 acatccagcg gcttcacttc gccattttca aggatacggc cattgccgac agccagcact
     2401 tcgccgcggg tggatttagc cgctgcagag ccggtcagaa cgatgccgcc agcagattta
     2461 gtttcaactt ctttacgctt gacgatcacg cgatcatgca atggacgaat attcattgat
     2521 aactctcctt tgagaaagtc cgtatctgtt atgggtgacg ccggagctta cgtggtttcc
     2581 cggctggtga ccagagaaat ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa
     2641 aaattctgat cagcacaaaa tcgggtgaaa accctgattc acctcacatt tcatcgcaat
     2701 ttcttcatcg ccgtcataag cgaatctgat tgtgctacca tcgaaaatct acgcagttgc
     2761 ccaaaatttg ggcgcaatcg gatatcaacc catgagtgga ctcaaacaag aactggggct
     2821 ggcccaaggc attggcctgc tatcgacgtc attattaggc actggcgtgt ttgccgttcc
     2881 tgcgttagct gcgctggtag cgggcaataa cagcctgtgg gcgtggcccg ttttgattat
     2941 cttagtgttc ccgattgcga ttgtgtttgc gattctgggt cgccactatc ccagcgcagg
     3001 cggtgtcgcg cacttcgtcg gtatggcgtt tggttcgcga cttgagcgag tcaccggctg
     3061 gttgttttta tcggtcattc ccgtgggttt gcctgccgcg ctacaaattg ccgccgggtt
     3121 cggccaggca atgtttggct ggcatagctg gcaactgttg ttggcagaac tcggtacgct
     3181 ggcgctggtg tggtatatcg gtactcgcgg tgccagttcc agtgctaatc tacaaaccgt
     3241 tattgccgga cttatcgtcg cgctgattgt cgctatctgg tgggcgggcg atatcaaacc
     3301 tgcgaatatc cccttcccgg cacctggtaa tatcgaactt accgggttat ttgctgcgtt
     3361 atcagtgatg ttctggtgtt ttgtcggtct ggaagcattt gcccatcttg cctcggaatt
     3421 taaaaatcca gagcgtgatt ttcctcgcgc attgatgatt ggtctgctgc tggcaggatt
     3481 agtctactgg ggctgtacgg tagtcgtctt acacttcgac gcctatggtg aaaaaatggc
     3541 ggcggcagca tcgcttccaa aaattgtagt gcagttgttc ggtgtaggag cgttatggat
     3601 tgcctgcgtg attggctatc tggcctgctt tgccagtctc aacatttata tacagagctt
     3661 cgcccgcctg gtctggtcgc aggcgcaaca taatcctgac cactacctgg cacgcctctc
     3721 ttctcgccat atcccgaata atgccctcaa tgcggtgctc ggctgctgtg tggtgagcac
     3781 gttggtgatt catgctttag agatcaatct ggacgctctt attatttatg ccaatggcat
     3841 ctttattatg atttatctgt tatgcatgct ggcaggctgt aaattattgc aaggacgtta
     3901 tcgactactg gcggtggttg gcgggctgtt atgcgttctg ttactggcaa tggtcggctg
     3961 gaaaagtctc tatgcgctga tcatgctggc ggggttatgg ctgttgctgc caaaacgaaa
     4021 aacgccggaa aatggcataa ccacataatc cggcgtttcg acattaatcc tggcgatcgt
     4081 ctttatgatc aaggcggtcg cgctcatcat cctttcgctg gtactcacca tcaaaagtat
     4141 taccgccacc ggtcccggcg ctaaaaccgc cgcctggcat acgagaaaag cgcaaatgcg
     4201 gcatcaactt cactgtcaga tgcttttgca ccggcggtaa taaaagcaga agaccgagga
     4261 agtcggtgaa aaagcccggt aataaaagca gcaaaccggc aatgatcagc gaaacacttt
     4321 taatcatctc cgccgctggg ttttcacccg ccgccatttt ttgctgcatc agcacgaaat
     4381 tcttaaagcc ctggttacgt accagtgaca tcccgataac tgacgtgaat ataaccagca
     4441 cgagggtcag caataccccc aatacatggg caacctgaat aaagattgaa atctcaatat
     4501 agacataaag gaaaatggca ataaaaggta accagcgcaa aggtttctcc tgtaatagca
     4561 gccggtaaaa cccggctacc tgaatgggtt gcgaatcgcg ttcagcttat attgtggtca
     4621 ttagcaaaat ttcaagatgt ttgcgcaact atttttggta gtaatcccaa agcggtgatc
     4681 tatttcacaa attaataatt aaggggtaaa aaccgacact taaagtgatc cagattacgg
     4741 tagaaatcct caagcagcat atgatctcgg gtattcggtc gatgcagggg ataatcgtcg
     4801 gtcgaaaaac attcgaaacc acatatattc tgtgtgttta aagcaaatca ttggcagctt
     4861 gaaaaagaag gttcacatgt caaacaacat tcgtatcgaa gaagatctgt tgggtaccag
     4921 ggaagttcca gctgatgcct actatggtgt tcacactctg agagcgattg aaaacttcta
     4981 tatcagcaac aacaaaatca gtgatattcc tgaatttgtt cgcggtatgg taatggttaa
     5041 aaaagccgca gctatggcaa acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc
     5101 catcattgcc gcatgtgatg aagtcctgaa caacggaaaa tgcatggatc agttcccggt
     5161 agacgtctac cagggcggcg caggtacttc cgtaaacatg aacaccaacg aagtgctggc
     5221 caatatcggt ctggaactga tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa
     5281 cgaccatgtt aacaaatgtc agtccactaa cgacgcctac ccgaccggtt tccgtatcgc
     5341 agtttactct tccctgatta agctggtaga tgcgattaac caactgcgtg aaggctttga
     5401 acgtaaagct gtcgaattcc aggacatcct gaaaatgggt cgtacccagc tgcaggacgc
     5461 agtaccgatg accctcggtc aggaattccg cgctttcagc atcctgctga aagaagaagt
     5521 gaaaaatatc caacgtaccg ctgaactgct gctggaagtt aaccttggcg caacagcaat
     5581 cggtactggt ctgaacacgc cgaaagagta ctctccgctg gcagtgaaaa aactggctga
     5641 agtcactggc ttcccatgcg taccggctga agacctgatc gaagcgacct ctgactgcgg
     5701 cgcttatgtt atggttcacg gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg
     5761 taacgacctg cgcttgctct cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc
     5821 ggaactgcag gcgggctctt ccatcatgcc agctaaagta aacccggttg ttccggaagt
     5881 ggttaaccag gtatgcttca aagtcatcgg taacgacacc actgttacca tggcagcaga
     5941 agcaggtcag ctgcagttga acgttatgga gccggtcatt ggccaggcca tgttcgaatc
     6001 cgttcacatt ctgaccaacg cttgctacaa cctgctggaa aaatgcatta acggcatcac
     6061 tgctaacaaa gaagtgtgcg aaggttacgt ttacaactct atcggtatcg ttacttacct
     6121 gaacccgttc atcggtcacc acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg
     6181 taagagtgta cgtgaagtcg ttctggaacg cggtctgttg actgaagcgg aacttgacga
     6241 tattttctcc gtacagaatc tgatgcaccc ggcttacaaa gcaaaacgct atactgatga
     6301 aagcgaacag taatcgtaca gggtagtaca aataaagaag gcacgtcaga tgacgtgcct
     6361 tttttcttgt gagcagtaac ttaaaaataa caacctaata tcaacttgtt aaaaaacaag
     6421 gaaggctaat atgctagttg tagaactcat catagttttg ctggcgatct tcttgggcgc
     6481 cagattgggg ggaataggta ttggttttgc aggcggattg ggggtgctgg ttcttgccgc
     6541 tattggcgtt aaacccggta acatcccgtt cgatgtcatt tccattatca tggcggttat
     6601 cgccgctatt tctgccatgc aggttgctgg cggtctggac tatctggttc atcagacaga
     6661 aaagctgctg cgccgtaacc cgaaatacat cacgatcctc gcaccgatcg tgacctattt
     6721 cctgactatc tttgctggta ctggcaacat atctctggcg acactgccag ttatcgctga
     6781 agttgcgaag gaacaaggcg tcaaaccttg ccgtccgctg tctactgcag tggtatccgc
     6841 gcagattgcg atcaccgcat cgccaatctc agcggcagtg gtttacatgt cttccgtgat
     6901 ggaaggtcat ggcatcagct acctccatct gctctccgtg gtcatcccgt ccaccctgct
     6961 ggcggttctg gtgatgtcct ttctggtcac tatgctattc aactccaaac tctctgacga
     7021 tccgatttat cgcaagcgtc tggaagaggg cctggttgaa ctgcgcggtg aaaagcagat
     7081 tgaaatcaaa tccggtgcaa aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt
     7141 ggttatctat gcaatcatca acagcccaag catgggtctg gttgaaaaac cgctgatgaa
     7201 caccaccaac gcaatcctga tcatcatgct cagcgttgca actctgacca ccgttatctg
     7261 taaagtcgat accgacaaca tcctcaactc cagcaccttc aaagcaggta tgagcgcctg
     7321 tatttgtatc ctgggtgttg cgtggctggg cgatactttc gtttccaaca acatcgactg
     7381 gatcaaagat accgctggtg aagtgattca gggtcatccg tggctgctgg ccgtcatctt
     7441 cttctttgct tctgctctgc tgtactctca ggctgcaacc gcaaaagcac tgatgccgat
     7501 ggccctggca ctgaacgttt caccgctgac cgctgttgct tctttcgctg cggtgtctgg
     7561 tctgttcatt ctgccgacct acccgacgct ggttgctgcg gtacagatgg atgacacggg
     7621 tacaacccgt atcggtaaat tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg
     7681 tgttgccctg gccgtttgct tcggcttcgt gctgggtagc ttcatgctgt aatgaccaat
     7741 cgcggggcgt tcacgccccg ctttctttcc cgccgactaa catccttccc ccgtccgttg
     7801 tatagtgacc tctctcttgc ggttccatct gttcttgcga ggtgtttatg cttgatgaaa
     7861 aaagttcgaa taccgcgtct gtcgtggtgc tatgtaccgc accggatgaa gcgacagccc
     7921 aggatttagc cgccaaagtg ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg
     7981 gcgctacctc tctctattac tgggaaggta agctggagca agaatacgaa gtgcagatga
     8041 ttttaaaaac taccgtatct caccagcagg cactgctgga atgcctgaag tctcatcatc
     8101 catatcaaac cccggaactt ctggttttac ctgttacaca cggagacaca gattacctct
     8161 catggctcaa cgcatcttta cgctgatcct gctactttgc agcacttccg tttttgccgg
     8221 attattcgac gcgccgggac gttcacaatt tgtccccgcg gatcaagcct ttacttttga
     8281 ttttcagcaa aaccaacatg accttaatct gacctggcag atcaaagacg gttactacct
     8341 ctaccgtaaa cagatccgca ttacgccgga acacgcgaaa attgccgacg tgcagctgcc
     8401 acaaggcgtc tggcatgaag atgagtttta cggcaaaagc gagatttacc gcgatcggct
     8461 gacgcttccc gtaaccatca accaggcgag tgcgggagcg acgttaactg tcacctacca
     8521 gggctgtgct gatgccggtt tctgttatcc gccagaaacc aaaaccgttc cgttaagcga
     8581 agtggtcgcc aacaacgcag cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac
     8641 cgcgcaattg cccttttccg cgctctgggc gttgttgatc ggtattggta tcgcctttac
     8701 gccatgcgtg ctgccaatgt acccactgat ttctggcatc gtgctgggtg gtaaacagcg
     8761 gctctccact gccagagcat tgttgctgac ctttatttat gtgcagggga tggcactgac
     8821 ttacacggcg ctgggtctgg tagttgccgc cgcagggtta cagttccagg cggcgctaca
     8881 gcacccatac gtgctcattg gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt
     8941 tggcttgttt actctacaac tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa
     9001 tcgccaacag ggcggctcac ctggcggtgt gtttgttatg ggggcgattg ccggactgat
     9061 ctgttcacca tgcaccaccg caccgcttag cgcgattctg ctgtatatcg cccaaagcgg
     9121 gaacatgtgg ctgggcggcg gcacgcttta tctctatgcg ttgggcatgg gcctgccgct
     9181 gatgctaatt accgtctttg gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca
     9241 agtcaaaacc gcgtttggtt ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt
     9301 gattggtgat gtatggggat tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg
     9361 ggcctttatc accagcctac aggctaaacg cgactggatg cgcgtggtgc aaataatcct
     9421 gctggcagcg gcattggtta gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca
     9481 taccgcgcaa actcagacgc atctcaactt tacacaaatc aaaacggtag atgagttaaa
     9541 tcaggcgctc gttgaagcca aaggcaaacc ggtgatgtta gatctttatg ccgactggtg
     9601 cgtcgcctgt aaagagtttg agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt
     9661 agcagacacg gtcttacttc aggccaacgt cacggccaac gacgcacaag atgtggcgct
     9721 gttaaagcat cttaatgtcc ttggcctacc gacaattctc ttttttgacg gacaaggcca
     9781 ggagcatcca caagcacgcg tcacgggctt tatggatgct gaaaccttca gcgcacattt
     9841 gcgcgatcgc caaccgtgaa cgacacttgc agtgggaaag acggaggaaa atagcgtgca
     9901 acgtgaagat gtactgggag aagccctgaa attattagaa ttacaaggga ttgccaacac
     9961 cacgctggag atggttgctg aacgtgtgga ttatccactg gacgagctac gccgcttctg
    10021 gccagacaaa gaggcaatcc tctacgatgc gctgcgttat cttagccaac agatagatgt
    10081 ctggcgtcgt cagctgatgc tggacgaaac gcaaaccgcc gaacaaaagt tgctggcacg
    10141 ttatcaggcg ttatcggagt gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc
    10201 tgcctgtacg ttttatcccg atcctggcca ccctattcat caactggccg atcagcaaaa
    10261 aagcgcggcc tacgatttca cccacgaact gttaaccacg ctggaagttg acgatccggc
    10321 gatggtagca aagcagatgg aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa
    10381 tcgtagccag gcggatgtcg acactgcaca tcggctggcg gaagatatcc tgcgcttcgc
    10441 ccgctgccgc cagggtggtg cattgacctg acagaaacac agaaaagaag cgatttgccg
    10501 caatcttaag cagttgaatc gcttttactg aaattaggtt gacgagatgt gcagattacg
    10561 gtttaatgcg ccccgttgcc cg
//
LOCUS       DAHBHC010000123        10451 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHBHC010000123 DAHBHC010000000
VERSION     DAHBHC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.124"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            456..1601
                     /locus_tag="M2A35_004552"
                     /pseudo
     CDS             456..1601
                     /locus_tag="M2A35_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            complement(1777..2079)
                     /locus_tag="M2A35_004553"
     CDS             complement(1777..2079)
                     /locus_tag="M2A35_004553"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200762.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2166..2981)
                     /gene="sul2"
                     /locus_tag="M2A35_004554"
     CDS             complement(2166..2981)
                     /gene="sul2"
                     /locus_tag="M2A35_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCB7200763.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3311..3487
                     /locus_tag="M2A35_004555"
     CDS             3311..3487
                     /locus_tag="M2A35_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCB7200764.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(3669..4424)
                     /locus_tag="M2A35_004556"
                     /pseudo
     CDS             complement(3669..4424)
                     /locus_tag="M2A35_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(4477..5181)
                     /locus_tag="M2A35_004557"
     CDS             complement(4477..5181)
                     /locus_tag="M2A35_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCB7200765.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(5383..6171)
                     /locus_tag="M2A35_004558"
     CDS             complement(5383..6171)
                     /locus_tag="M2A35_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCB7200766.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(6302..6775)
                     /locus_tag="M2A35_004559"
     CDS             complement(6302..6775)
                     /locus_tag="M2A35_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HCB7200767.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            6933..7946
                     /gene="intI1"
                     /locus_tag="M2A35_004560"
     CDS             6933..7946
                     /gene="intI1"
                     /locus_tag="M2A35_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCB7200768.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            8149..8499
                     /locus_tag="M2A35_004561"
     CDS             8149..8499
                     /locus_tag="M2A35_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCB7200769.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            8675..9235
                     /locus_tag="M2A35_004562"
     CDS             8675..9235
                     /locus_tag="M2A35_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7200770.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            9239..>10451
                     /locus_tag="M2A35_004563"
     CDS             9239..>10451
                     /locus_tag="M2A35_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCB7200771.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
BASE COUNT         2324 a         2989 c         2974 g         2164 t
ORIGIN      
        1 attaactgag agccattacg gtccatgctt tttgcagcgg ccataaaagc atccttcagt
       61 tcagcgtcga ttcttaaact catattaacc tgtgccattt atcacctcgt cattactacg
      121 tatatacaca gtatataacg attcagatat cgtcacaagc gaagcgcgga ggccgcaggc
      181 cggaggcatt agtggccgcc gcccgaaggg gcgagacgcg tagcggctcg atgcgcagca
      241 cggcagaacg gccccgcagg ggtaatgccc agtttaattc aacgtgacag tcacgtggag
      301 gaaaaatatg aatgaccgac agcgtgaaca agcccgtatt cgccaggccc ggcgccgcgc
      361 gcgactcaaa gaggaaggcg ctagcgtgac agtcacgcta acaaaacagg aagaagcaat
      421 gttacaggag ctttgccggg ttcgccgtcc aggacgaaca acccatgcgt cagtgggtgt
      481 tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg
      541 tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc
      601 accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc
      661 tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct
      721 cagcgcgttt ccgctgggtc aaggcgccga ccaccccaga gctcacccag ctgacgcaca
      781 ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg
      841 aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc
      901 actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc
      961 aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca
     1021 gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc
     1081 ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg
     1141 tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat
     1201 tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaacccgct
     1261 tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg gcaaaacggg
     1321 gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa cggcgagcgt
     1381 cgatgacatg ggcgcaacgg ctcaagcgtg ttttcaatat cgacatcgag acctgcagcg
     1441 gctgcggcgg cgccatgaaa gtcatcgcct gcattgaaga ccctatagtg atcaagcaga
     1501 tccttgatca cctgaagcac aaagccgaaa ccagcgggac cagggcgtta cccgaaagcc
     1561 gggcgccacc ggctgagctg ctcctgggtc tgtttgactg acgagcctga aggccaacga
     1621 taccaatcaa aatgctgcgt tcacagcgcc gcggcaggga tccgccgtgc tggttgtcgg
     1681 aaaaggagcc gctagtggga aagaggaggg taaattttca gcgttgctgg ctccccgtca
     1741 gccggattgg gttgcatcgc aggggtgtcg aaagagtcaa ctgcggtcca aagctgttgg
     1801 acttgggtga aaagggcgtt tattcttcct atacgttgtc ggcagcgggc caaaaaggaa
     1861 tacgtccatg cccatcgagg tgaaaccggc tgtgagcgcg ggttcaagca tatagcccga
     1921 caggcgcgta tccttgccga tcacgacacg atggcggtgg tcaccgcgac gaaagacacg
     1981 gccagccgcc atgccgacgc gcaaggcggt ttccgccgtc atcgcgcctt cgttggcttt
     2041 gccacgaata ccgtctgtgc cgaaatattt gcgcaccata aggtcgatta tcctgtcgtc
     2101 gggtcgccct caaaggggac atgcctgctg aaccgcgaat atagagaaat atcccgaatg
     2161 tgcagttaac gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc
     2221 aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct
     2281 gcagcgagtg tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag
     2341 gatttgcgcg aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac
     2401 cgcgccagca ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga
     2461 tcaaggacaa ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca
     2521 aagaacgccg caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc
     2581 ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag
     2641 aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca
     2701 taggcttgcg tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc
     2761 gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg
     2821 ggattgctgg atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc
     2881 gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag
     2941 gttatgttga cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata
     3001 ataatcgagc atgagtctca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa
     3061 cctgtttgcg gtgatccatt agctgcagga gcagaatagc atacatctgg aagcaaagcc
     3121 aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt
     3181 taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca
     3241 ttgaatataa aagataaaaa tgtcttgttt acaatagagt gggtatcgga gacgaagaag
     3301 gacaaggtac atgctgaccg ataccaagct gcgcaatctc aagcccaggg acaaactcta
     3361 caaagtgaat gaccgggaag gtctctatgt gggggtcgcc tcagaaaacg gaatctatgg
     3421 tcactcccgt ttttgcaaca ccgattttga cgacaagttg gcttgcttga atctatccgg
     3481 cgtctgaatg ggattttatt cccgcgcctc gatgagttcc gcgcctgatg aacctccaga
     3541 aaatatacgg cttcaatgag cctttccgtt ttacaggttc ctcaacaggc cggtgggccg
     3601 ttagtatcat caatatcagt attcgcaaaa ccagatgaat gattgtttaa actggtgtat
     3661 ttctgccttt atgcttcgta agtttgctgt cgcgccgtca gtgcccaggc tattctggcc
     3721 agcttgtttg ccagagcaca ggtgacgaca aagttgcttt tccgacacaa caactccctg
     3781 acccagtcgg ccaacttgcc agactggtgt tccagttttt gtatgaatac cctggcacac
     3841 tgaaccaaca aagttcggat ctttttgttg ccccgcttgc taatccctaa caatgtcgtc
     3901 cgacctcccg tgctgtactg tcggggtacc agccctgttg ccgccgcaaa gtcacggctg
     3961 ctggcgtact gcttcccgtc gccaatctca gttgaaatag tactggcagt cagcgttcca
     4021 acgcagggaa tactcagcaa gcgctgtcca acctcatctt cgtccaactt tcgtttcaac
     4081 tgagattcca gatctttaat ctgctcaaca agatagtgat aatgctgttg taatttcagc
     4141 agtaactggc tgagataaag aggcaaacta ctgtcctcaa gaagggtact cagtcgacta
     4201 ataacggcag cacctcgcgg aacgctgata ccaaattcca gcagaaaagc atgcatctga
     4261 ttagttgttt tcaccttatc ctgaaccagg gattcacgga cacgatgcag agctcgcatt
     4321 gcctgctgag attcggttct gggctgcacg aaacgcatag atggacgtga tgctgcttca
     4381 cagatagctt cagcatcaac gaagtcattt ttgttgcttt taacggcact gttgcaaagt
     4441 tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac
     4501 caggcgcatt tcgcccaggg gatcaccata ataaaatgct gaggcctggc ctttgcgtag
     4561 tgcacgcatc acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc
     4621 cagcgtggcg ccgattatcc gtttcagttt gccatgatcg cattcaatca cgttgttccg
     4681 gtacttaatc tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc
     4741 aagcgcgcga ccataggcgg gcgctttatc cgtgttgatg aatcgcggga tctgccactt
     4801 cttcacgttg ttgaggattt tacccagaaa ccggtatgca gctttgctgt tacgacggga
     4861 ggagagataa aaatcgacag tgcggccccg gctgtcgacg gcccggtaca gatacgccca
     4921 gcggccattg accttcacgt aggtttcatc catgtgccac gggcaaagat cggaagggtt
     4981 acgccagtac cagcgcagcc gtttttccat ttcaggcgca taacgctgaa cccagcggta
     5041 aatcgtggag tgatcgacat tcactccgcg ttcagccagc atctcctgca gctcacggta
     5101 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     5161 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     5221 ccaccgacta tttgcaacag tgccgatagt ttggctgtga gcaattatgt gcttagtgca
     5281 tctaacgcat agttgagcgg cgggcgcagc ccgtccgctt gaacgccgag ttaggcatca
     5341 gatgccctcg gcgcgggtcg atgcactttt cgcacatgcc gctcaacgca agattctctc
     5401 aatcgttgct ttggcatatc gaacgaacgc ggccgtctct tcgacgcgca ttgctaggtc
     5461 gtcgtcctcg ctacccaggt acgccgcgcg tgccttgcag atgaggggcc gatgctcggc
     5521 aggcaaacgc tccgataccc atgcggcagc aacgtcctta ggagcaatga gaccagttga
     5581 agcgctgtac caaatgcgag caagagcaag aacgacgttc cgctcgtcac ccttccaatc
     5641 cgactctgca ttccactggg caatagtgtc gaaaagcgcc ttggagaaat gctccttcgg
     5701 caccggctcg aaaaacgtgg ctgcggatgg gcctagaagc gcaaggctgt gttgcctcgc
     5761 cttggtcagc aaaatcgcaa gatcgtgatc cagaacggca ggctcgaacg ttccggaaag
     5821 gatgtcgtgg cggagccact caccgaactg aagctcacgc cgcgccggat agcgccaagg
     5881 cactacttcg cttcgagcga caacagttag ctccagcggt cgccatgttc cgccatcgcc
     5941 tggcggtgat gagactttca gcaaatcgag cattagcgcc tgccggagcg aatcgttagg
     6001 tgcggcgctg acggtcacga gcaagtctat gtcgctgtcc ggcttcagcc ctccatcgat
     6061 cgcagatccg aacaggtgga ttgtgtccag tgtcgcagcc agatggcgct cgatcaccgc
     6121 gcgagcgtgg gacagctgct tgaaaacttg tgcagggaaa aattcaccca tgatgcctaa
     6181 cgttaagttc agcggcagct tttaagttgc ggctttgtgg aatacttttg cgcagcaaaa
     6241 ccacaaagac gcgacttaaa agctgtccaa ggagcgaagc gactggtgct gcaacgcatt
     6301 gttagccttt tttccaaatc tggtatgtat aatttatatt agacataaaa aactgttcaa
     6361 aaaccaaatt gaaattctca ggcattatag ggaatttgat atcaccttcg acttcaacgt
     6421 gaacagtaga caaatgaatt atatctgctt tttcaataag gctattatag atttgacccc
     6481 cgccagagac atatacatga tctgtaactt ttgatagctc tttcaaagca ttttctattg
     6541 aaggaaaaac taggacgttt tcatttgagc ttgaaattcc gttctttgac actactgcat
     6601 atttgcgatt tggaagaaca cccatagagt caaatgtttt tcttccgaca aggagccatt
     6661 gattatatgt gagcgcttta aagagtagtt gctcaccttt tactgaccac gggatatcag
     6721 gaccactacc gattacgcca ttttctgaca ctgcagaaat caatgatatt ttcaatttaa
     6781 ctcccttaat ggctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     6841 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     6901 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     6961 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     7021 gtttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg
     7081 tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga
     7141 acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct
     7201 acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt
     7261 cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg
     7321 aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg
     7381 gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg
     7441 gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg
     7501 agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg
     7561 ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt
     7621 tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc
     7681 atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca
     7741 tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca
     7801 gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga
     7861 tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc
     7921 tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc
     7981 cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt
     8041 ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata
     8101 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta
     8161 gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg
     8221 gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac
     8281 caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc
     8341 aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc
     8401 tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc
     8461 gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta acttgatgtc
     8521 aggcagggcc gcgccgcttc gtcagaatag agtctgcttt cccatttttt gacacatgcc
     8581 cgcgaaggtt atagatttca gcctgacaga aatgggcttt gaggcacaac ggaacagaaa
     8641 gtgcacttaa gccgccttca accaaggaga catcgtgcag gggcaccgca tcggctacgt
     8701 ccgggtcagc agctttgacc agaacccgga acgccagctg gaacaaaccc aggtgagcaa
     8761 ggtgttcacc gacaaggcat cgggcaagga cacccagcgc ccccagctcg aagcgctgct
     8821 gagcttcgtc cgcgaaggcg atacagtggt ggtgcacagc atggatcggc tggcccgcaa
     8881 cctcgatgac ctgcgtcgct tggtacagaa gctgactcag cgcggcgtgc gcatcgagtt
     8941 cctgaaggag ggcctggtgt tcactggcga ggactcgccg atggccaacc tgatgctgtc
     9001 ggtgatgggg gccttcgctg agttcgagcg cgccctgatc cgcgagcggc agcgtgaggg
     9061 catcgccttg gccaagcagc gtggcgcgta ccggggccgc aagaaagccc tgtccgatga
     9121 gcaggctgct accctgcggc agcgagcgac ggccggcgag cccaaggcgc agcttgcccg
     9181 cgagttcaac atcagccggg aaaccctcta ccagtacctc cgcacggacg actgacacat
     9241 gccgcgtcgc ttgatcctct cggccacgga gcgggacacc ctgcttgcgc tgccggaaag
     9301 ccaggatgac ctgatccgct actacacctt caacgactcc gacctgtcgc tgatccgcca
     9361 gcgacgcggc gacgccaacc gcctcggctt cgccgtgcag ctctgcctgc tgcgctaccc
     9421 cggttacgcg ctgggaaccg acagcgagct gcccgagccg gtcatcctgt gggtggcgaa
     9481 gcaagtccag gccgagccgg cgagctgggc aaagtacggc gagcgcgacg tgacccgtcg
     9541 cgagcatgcc caggaactgc gcacctacct gcaactggcc ccgttcggcc tgtccgactt
     9601 ccgcgccctg gtgcgcgagc taaccgagct ggcccagcag accgacaaag gcttgctgct
     9661 ggccggtcag gccctggaga gcctacggca gaaacgacgc atcctgccgg cgctgagcgt
     9721 gattgaccgg gcctgctcgg aagccattgc gcgagccaat cggcgggtct accgcgccct
     9781 ggtcgaacca ctcacggact cgcatcgggc caagctggac gagctgttga agctcaaggc
     9841 cggcagcagc atcacctggt tgacctggct gcgccaggca ccgctgaaac ccaactctcg
     9901 gcacatgctg gaacacatcg agcggctgaa gacatttcag ttggtggact tgcccgaagg
     9961 cctgggccgg cacatccacc agaaccgcct gctcaagctg gcccgcgagg gtgggcagat
    10021 gacgcccaaa gacctcggta agttcgagcc gcagcgccgc tacgcgaccc tggccgccgt
    10081 ggtgctggag agcaccgcga ccgtgatcga tgagctggtc gatctgcatg accgcatcct
    10141 ggtcaagctg ttcagcggcg cgaagcacaa gcatcagcag cagttccaga agcagggcaa
    10201 ggcgatcaac gacaaggtgc gcctgtactc caggatcggc caggcgctgc tggaagcgaa
    10261 ggaaagcggc agcgacccct atgccgccat cgaggcggtg attccctggg acgagttcac
    10321 cgagagcgtc agcgaggccg agctgctggc ccggccggaa ggcttcgacc acctgcacct
    10381 ggtcggcgag aacttcgcca ccctgcgccg ttacacgccg gccttgctgg aggtgctgga
    10441 actgcgcgcc g
//
LOCUS       DAHBHC010000124        10395 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHBHC010000124 DAHBHC010000000
VERSION     DAHBHC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.125"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1816)
                     /locus_tag="M2A35_004564"
     CDS             complement(<1..1816)
                     /locus_tag="M2A35_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCB7200772.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTVYWNPPTLNGVLMKLSVGQYLAK"
     gene            complement(1816..2562)
                     /locus_tag="M2A35_004565"
     CDS             complement(1816..2562)
                     /locus_tag="M2A35_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCB7200773.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLHITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(2559..3203)
                     /locus_tag="M2A35_004566"
     CDS             complement(2559..3203)
                     /locus_tag="M2A35_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCB7200774.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(3310..3615)
                     /locus_tag="M2A35_004567"
     CDS             complement(3310..3615)
                     /locus_tag="M2A35_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200775.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(4057..4269)
                     /gene="cspH"
                     /locus_tag="M2A35_004568"
     CDS             complement(4057..4269)
                     /gene="cspH"
                     /locus_tag="M2A35_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCB7200776.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            4555..4767
                     /gene="cspG"
                     /locus_tag="M2A35_004569"
     CDS             4555..4767
                     /gene="cspG"
                     /locus_tag="M2A35_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCB7200777.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            4778..4966
                     /locus_tag="M2A35_004570"
     CDS             4778..4966
                     /locus_tag="M2A35_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCB7200778.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            4941..5171
                     /gene="ymcE"
                     /locus_tag="M2A35_004571"
     CDS             4941..5171
                     /gene="ymcE"
                     /locus_tag="M2A35_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCB7200779.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFREM
                     AYNISYLTRGPGTNTLLACCCLLRPEEVNSEY"
     gene            5161..5334
                     /locus_tag="M2A35_004572"
     CDS             5161..5334
                     /locus_tag="M2A35_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCB7200780.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(5383..6456)
                     /locus_tag="M2A35_004573"
     CDS             complement(5383..6456)
                     /locus_tag="M2A35_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCB7200781.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPTIGH"
     gene            complement(6528..9272)
                     /gene="torS"
                     /locus_tag="M2A35_004574"
     CDS             complement(6528..9272)
                     /gene="torS"
                     /locus_tag="M2A35_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCB7200782.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPEPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLVQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANTDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMCSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            complement(9367..10389)
                     /locus_tag="M2A35_004575"
     CDS             complement(9367..10389)
                     /locus_tag="M2A35_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7200783.1"
                     /translation="MRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQANIELLSDSGR
                     ESLIDRIKGADVITLHAWLDRYETILSERGIRPKTLLDYASKIRAIRRKLPDKPIADI
                     STKEVAAMLNTYVAEGKSASAKLIRSTLVDVFREAIAEGHVATNPVTATRTAKSEVRR
                     SRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNHLHIEQSK
                     TGAKLAIPLTLRIDALNISLADTLQKCRKASGSETIIASTHHEPLSPKTVSKYFTKAR
                     NASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRDSRGREWD
                     KIEIDK"
BASE COUNT         2573 a         2611 c         2528 g         2683 t
ORIGIN      
        1 cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt
       61 tccagtaaac cgtcacgaaa cctgttggcg tggaataatc ggtgaagcgc atcatgttgt
      121 cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagctg gcatctagcg
      181 ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa ccgccataca
      241 cctggccata gaaaccattg cctaagtcgg caaagtagtt cgcctgcaag ttgttgagat
      301 aaacgtcatt gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg gtagagtccg
      361 cgggtaatag cgaagactta aacttgtcgt agttattgta aatattggtg aaaataccgc
      421 catcaagcag caggtggtca gtaaaccagt agcgggcgct ggacatcagc cccaactgga
      481 acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc
      541 taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct tcagcttcaa
      601 cacgttgttg ttgcagtggc tctgattgac caggcgctgt accggccagc tgtttgcgca
      661 ggctggctac gtcggtttcg gtagtcacca tagccatatg ctcgcgcttt tgcgtcacgc
      721 taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg
      781 cttcacgaga gtcacggtat ttgtattgct gaccagacat atacagcgtc ttatcgcgta
      841 gctgaatttc tggcgcgtca aagcccgcgt tatacttcag ggcggtaagt tggtttgcta
      901 ccgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat
      961 cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa agtgaaacca aacatcaacg
     1021 tgttaccgcg ttcataactc aggttgaggt ctgcccagct ggcagcgcga taaactgcac
     1081 cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc
     1141 cgtcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa
     1201 agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgagact
     1261 ctgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat
     1321 taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg
     1381 ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcta
     1441 cttgcggtag ccagtaacct tcttcccata ggcgaagttt aaaatcgaaa gatttgtctt
     1501 tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg cgcacatccg
     1561 tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaacggt
     1621 attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg
     1681 gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc ggatccgggt
     1741 aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat
     1801 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
     1861 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
     1921 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
     1981 ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg
     2041 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccagtcc cagaaaccgc
     2101 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccag
     2161 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
     2221 accagtgata tggagattga gtaactgctg gcgcacggat ttaatcgttg ctgccacatc
     2281 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
     2341 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
     2401 cagttgcggt tgtgtcacca actggacaac attttccacg gggccaacgg aaagtgtctg
     2461 ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
     2521 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
     2581 gtcatttcta cgggaataac ccctgcgcct aacatttgtc gactttgacg aacctggcca
     2641 gtagccgaat cgacccagaa tatgttttgc cactgttttt ctggcagcgt ggaagtcacc
     2701 tcttcctgcc agacattaca cagcacatcg cgacccgcaa tattcagcgt ctctaatcca
     2761 gcaaaggaaa aagtggaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga
     2821 atatcgcgcg tccagcggct gccgtctttt attgccagcg cgttacgcag agggtcctgc
     2881 ccggaatttg tcacttccag cagattatta ttaagtttga cggtctttaa aagacgtcca
     2941 ttatgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
     3001 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat cgtggaataa
     3061 ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg
     3121 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
     3181 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
     3241 ctctaaagcc cggtacgaat atcgcaaccg ggtattattt aactttaatt tcgacgcgtt
     3301 taagcagaat tagcgcgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
     3361 actgtcattt gctgcaacta ctgccaacgt cgccacacca acagccgttg ccacaccgac
     3421 aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagcag ccgtcgtggt
     3481 tgcaccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc
     3541 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
     3601 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat
     3661 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgcttg
     3721 cactatatac agcgggtgag aaaggcgcgc agagggaaat ataagattgt taacgatttc
     3781 cctctggaaa tacgacttgt gagcataaga taaatcttag aaaaatacaa attaaagata
     3841 agaaaattgt taagaaagct attaactatt gttgataata tagtttcaag cggtactata
     3901 actggctgtt aagtggcatt tataactatt agttgcagag atattcggtt aaatggatta
     3961 gtttcctgat aaaaaagtaa cttgcgttat aatgcatggt gttgactatt gaactattcc
     4021 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
     4081 tgttggtcct cgcaggccat ttacgcgaca aaattccacg cgtaatcctg gtataagcac
     4141 ttctgcgtcg cggggagtga atgcggaaat atggacctgg acttctttac gaccgtcgga
     4201 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
     4261 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
     4321 aaagccagcg aaatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
     4381 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
     4441 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
     4501 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
     4561 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
     4621 cctgatgatg gcagcaaaga cgttttcgtc catttcaccg ccatccagag caatgaattc
     4681 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggcccc
     4741 gcggcagcga acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg
     4801 ctgtccatgt tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa
     4861 ccctttgggc gctaagtgta ttttttgtaa atcgacaatg atcacctttg ataacgtcgc
     4921 gctgcaaata cgcactgacc atgcgccgct ggatttcaca aaataatatc aggctccctc
     4981 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
     5041 acctgttgat tattgaatct ttcagggaga tggcttataa catttcttac ctgaccaggg
     5101 gaccgggaac caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca
     5161 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
     5221 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
     5281 cgggaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
     5341 gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcgttgg
     5401 cgcttgtgga attaagcgtc ggtacaattc ctccggcacc gggctttgcc atactcccgc
     5461 atacattgcg taaccaatca ccgcaaacat aatccccaga accagtagtg tcattaacca
     5521 gccagacaac gcaaaagctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt
     5581 tgaggctacc ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac
     5641 ctgaattaat ttatcgaccg ggatccgtga tgggcaattt tttgcgcatt tgccacagtc
     5701 gatacaactt tcggcattgc gacgaatctt aaacggcgat aatagcgaaa ccacgcccat
     5761 cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat
     5821 cagcaaaacg gtcacgctga ttaatgtcgc ggtccccata tgacgaaaga aatcgagcat
     5881 tttaacgtcc atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg
     5941 catcaataac gcgatataga gaaaaaaact caacagcaaa tacttcacgc cgcgcagagg
     6001 aatatccagc cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagatcgcc
     6061 gattaattca gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa
     6121 tagactgatg acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa
     6181 ctgacctgtg gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     6241 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     6301 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     6361 acgccaggtc gtggcattgc gccagttatt ccacggtaat ttgccgcccg tcgtgcctgg
     6421 ccgccgctgc cagcgggttc ttttattctc tgccatgatt ttgccagtcc gttaagttgc
     6481 ataccaaatg ccactattct agttgttctt aactggctga tattgattca aatcgcgttc
     6541 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     6601 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcactgcac
     6661 attcccagac tcgagcaact gctttttagc tgatgtgcgg cacgctttat tttctcgcta
     6721 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     6781 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     6841 gatacatcca gcggttgatc ggtattggct tgcagttgga gatagtgcgc cagtaactga
     6901 ccgagcactt cacgcggcac cggtttaggg ataatcccgc gaaataatga actggtacgc
     6961 tggcgcaacg tttcgtcgat gacatgagcg ctaaagccaa tcaaaaccag cgacggatat
     7021 tgctgtacca gttgtcgggc aagggtaatg ccgtcgatat ccggcagatc aaaatccacc
     7081 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     7141 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     7201 gggttatctt caattaacag caaacgtaaa ccgtccagac gcaccgcctg attggctgtt
     7261 tttggctcgg gtgcagtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     7321 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     7381 gtcagcccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     7441 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     7501 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg tagggcgttg
     7561 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcgctcggt
     7621 acatcatcgg caattgccgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     7681 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gttcatcgct gaccgaaaca
     7741 ttcttgccgc ctgcttcgat agccgaataa tcgagaatat cgttcaggat ggtcagcaac
     7801 gattcaccag agtcagtaat tgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     7861 tctgccagta gctgtgcggt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     7921 atcgccgcca gaaacgccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgg
     7981 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     8041 tgttcgcggt ggcgattcag tgcatgaaca ttgctgcgaa acgcatccat cagccgcccg
     8101 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     8161 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     8221 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     8281 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     8341 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     8401 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     8461 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     8521 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     8581 gttggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     8641 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     8701 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     8761 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     8821 atctcatcgg cggcagcgac tatctgctga ctgagttgcc gttgttgctg gcgtagttgc
     8881 agacgccgcc ccaccagttc cccttgctga cgtaatgaac gggagatctc ctgctcctgt
     8941 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     9001 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     9061 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     9121 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     9181 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     9241 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cacttaaggt gaataagttg
     9301 tatatttaaa atctctttga ctatcaatga attaatataa gcaggtcatt attagtcaaa
     9361 ataaaatcat ttgtcgattt caattttgtc ccattcccgt ccacggctgt ccctataccg
     9421 cgccgccatt gaatctgatt tatgcccgag aagacgttga gcaaacttat cgccaatctg
     9481 gtttcggtat agcctcgctg acaggctacg cagttcatgg aatgttggcg ggtctccatc
     9541 aaatgagagt ccagatgcat ttctcgcctt tgtaaaatac ttcgagactg ttttcgggga
     9601 aagtggttcg tgatgcgttg atgcaattat tgtttcactg ccgctggcct tcctgcattt
     9661 ctgtagtgta tcagccaatg agatattgag cgcgtcaatc cttagcgtca gcggaatggc
     9721 gagcttagcc cctgttttac tctgctcaat gtgaagatgg ttgtcgttta tgtctgacca
     9781 tttcattctg cataaatcgc ctactctctg ccctgtaacg acagccaaat ccattgccag
     9841 tctcagccag attgggagcg gttcggctgc atggtatatc gcgacatatt cattagctgt
     9901 cagtcttgaa cgccttactt ctgattttgc tgtgcgggtt gctgttaccg gattcgtagc
     9961 cacatgcccc tcggctattg cctcacgaaa aacgtcaaca agggttgacc tgattaattt
    10021 tgcggaagcc gatttgcctt ctgctacgta ggtgtttagc attgctgcca cctctttcgt
    10081 tgatatgtca gcgatcggtt tgtccggcaa ttttcttcgg attgccctga ttttgctggc
    10141 gtagtcgagt agggttttcg gcctgatccc cctctcgctg aggattgttt catatcggtc
    10201 aagccacgca tgaagagtga ttacgtcagc gcctttaatt ctgtctatca gtgactcgcg
    10261 cccgctgtct gatagcaact caatgttggc ctgtatagct tcagtgattg ctatcctcct
    10321 gtctcggcct aatccaaact ctttacccgt ccttgggtcc ctgtagcagt aatatccatt
    10381 gtttcttata taaag
//
LOCUS       DAHBHC010000125         9840 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHBHC010000125 DAHBHC010000000
VERSION     DAHBHC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.127"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..669)
                     /locus_tag="M2A35_004576"
     CDS             complement(<1..669)
                     /locus_tag="M2A35_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCB7200784.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFM"
     gene            complement(680..1681)
                     /gene="ltaE"
                     /locus_tag="M2A35_004577"
     CDS             complement(680..1681)
                     /gene="ltaE"
                     /locus_tag="M2A35_004577"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCB7200785.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1718..3436)
                     /gene="poxB"
                     /locus_tag="M2A35_004578"
     CDS             complement(1718..3436)
                     /gene="poxB"
                     /locus_tag="M2A35_004578"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCB7200786.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWEVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3569..4537)
                     /gene="hcr"
                     /locus_tag="M2A35_004579"
     CDS             complement(3569..4537)
                     /gene="hcr"
                     /locus_tag="M2A35_004579"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCB7200787.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4549..6201)
                     /gene="hcp"
                     /locus_tag="M2A35_004580"
                     /gene_synonym="priS"
     CDS             complement(4549..6201)
                     /gene="hcp"
                     /locus_tag="M2A35_004580"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCB7200788.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6345..7244)
                     /gene="lysO"
                     /locus_tag="M2A35_004581"
     CDS             complement(6345..7244)
                     /gene="lysO"
                     /locus_tag="M2A35_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCB7200789.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7739..8434)
                     /gene="aqpZ"
                     /locus_tag="M2A35_004582"
     CDS             complement(7739..8434)
                     /gene="aqpZ"
                     /locus_tag="M2A35_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCB7200790.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8860..>9840
                     /locus_tag="M2A35_004583"
     CDS             8860..>9840
                     /locus_tag="M2A35_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2813 domain-containing protein"
                     /protein_id="HCB7200791.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGEL"
BASE COUNT         2425 a         2628 c         2560 g         2227 t
ORIGIN      
        1 cataaaatgt tcaaactgtt gctgataact gagcacctct ggtccggcgg cttcgaagat
       61 gcggtgttcg ctggccggat gatctaacaa cgccaccaga tagtgcagca agttttccag
      121 cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc gttaacactg gcaggttgta
      181 gaccatatcg cgcatgactt cgaacgccgc tgaacctgcg ccaacgataa ttccggcccg
      241 aagttcggtc acaggtacat tcgcttcacg aagaatgtcc gccgtagcct gacgagcacg
      301 cagatgatcc gactgctcat gtggcggggc ctgcaacgaa ctgagaaaga ttaattgctt
      361 aactggtact tcacgtagcg catcgcggac gttgagagcc acctggcgct cctgagcgat
      421 aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag acggtatcga tatcctgcaa
      481 cagggccgga aggttatccg gccagctgag atcgacttta tggcaactga cgtttgccag
      541 ttgcagcttt gcaagcctgt cgacatgacg tgccgccgcc aggatctgat gcccttgctg
      601 gctgagtgtg cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg
      661 ttgcggcacg tctctctcct taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg
      721 ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa
      781 tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc
      841 gaacaaacag catattagtg tcctgacgca tcacatccgc gcctgcttca cgcagttgct
      901 ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg tgcgacgtta tttttcaggg
      961 catacatccc ggcggcagcc agaatcccgg actgacgcat cccgccaccg gccattttcc
     1021 gccagcggat ggcacgttta atgtaatcac gattaccgac gagtaatgaa ccgactggcg
     1081 tcccaagacc tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca
     1141 gttcgcagcc gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca
     1201 gattgcgctc gcgggtaaat tcccatgctt ctttcaggta ttcacgcggc agcactttgc
     1261 cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt
     1321 tgattttcat cgccacttta tccagcggta gcgtgccgtc ggcagccgcg tcgatgggtt
     1381 gcggctgaat actgcccagc accgctgcgc caccggcttc aaacagatag ttatgcgcgg
     1441 cctgaccgac aatatactct tcgccacgtt cgcagtgact gagcagagcg accaggttgg
     1501 cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcatagt
     1561 cctgcagagc attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca
     1621 tcatcgcttc gagcatggcg cggctcggtc gggtaacggt atcactgcgt aaatcaatca
     1681 tggcatgtcc ttattatgac gggaaatgcc acccttttta ccttagccag ttcgttttcg
     1741 ccagttcgat cacttcatcg ccgcgtccgc tgataattgc gcgcaacata tacaggctaa
     1801 aacctttggc ctgttcgagt ttgatctgcg gtggaatggc taactcttct ttggcgacca
     1861 ccacatccac caacaccgga ccgtcgatag agaaggcgcg ttgcagggct tcatcaactt
     1921 cagacgcttt ttctacacgg atacccgtaa tgccgcacgc ttcggcaatg cgggcaaagt
     1981 ttgtgtcgtg tagttcggtg ccgtcagtca aatagccacc agctttcatc tccatcgcca
     2041 caaagcccag cacgctgttg ttaaagacga caattttcac tggcagtttc atctgcacta
     2101 ctgagaggaa atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct
     2161 gacgttctgg ctctgtcgcc tgcgcaccca gcgcctgcgg catggcgtta gccatcgaac
     2221 cgtggttaaa cgaacctaac aggcgacgct tgccgttcat ttttagataa cgtgccgccc
     2281 acaccgttgg cgtaccaacg tcacaggtga aaatagcgtc atcggcggca aaatgactaa
     2341 tttgctgcgc cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca
     2401 gccctttgcg ggcgtcgcgg taatcttcca gcgctttatc cagaaacttg cgatcggctt
     2461 tttcttccac caatggaagc aatgcacgca gagtcgactt gatatcgccg accagtgcca
     2521 tatccacctt gctgtgagcg ccgatgctgg ctgggttgat atcaatctga atgattttgg
     2581 catcggtcgg gtagaaggcg cggtagggaa attgcgtgcc gagtagcact aacgtgtcgg
     2641 cgttcatcat ggtatggaaa cctgacgaga agccgattaa cccggtcatt ccaacatcat
     2701 acggattatc gtattcgaca tgttctttac cgcgcagggc atgaacaata ggcgctttaa
     2761 ttttcccggc aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca
     2821 gggcgatatt gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg
     2881 gcgtcacgac tggttgtggc gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt
     2941 ttaacgccac gtcgcctggt aacacgacaa ccgaaacgcc acggttaagc accgctttgc
     3001 gcatggcaat cgccagtact tgtgggatct gctccgggct ggaaaccaac tcgcaatagt
     3061 gactacattc gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgccaattt
     3121 cgctggaggg aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg tggcaatcga
     3181 acaggccgtt gattaagtgc aggttgccgg ggccgcacga tccggcgcag accgccagtt
     3241 ctccgctaag ttgtgcttca gcgccagcgg caaaggccgc cacttcttcg tggcgggtgg
     3301 acatccactc gatggtgccc atgcgattaa gactgtcact aagaccgttc agagagtcgc
     3361 cagtgacttc ccagatgcgt ttcacccctg ccgattcgag tgttttggcg atataagctg
     3421 caaccgtttg tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc
     3481 atctgacgga gggggaaggg atgggagaga aaggaggcac taacggttaa atagcccgat
     3541 gaaaggaata tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc
     3601 aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtactgc
     3661 tcaccgtata ttcaccggaa accacttttg tcttacagca gccgcaaaca cccgcacggc
     3721 aggcagcgac aaccggaacg ttattgcttt ccagcgcctc cagtagtgtg gtgccaaccg
     3781 gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt
     3841 ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt
     3901 cctgctctac ccaatccata tacggagccg ggccgcaggt catcacggta cgtgaagcta
     3961 agtcaggtac acctgccagc agttcgcgag tgagacgacc agcgataaag ccttctgtaa
     4021 cgttattttc tgccaccagc gttaccggat agttacgcca ctcatcggcg aaaataacat
     4081 cctgcggcgt acgcacgttg tagatcaccc gcacatcggc ctgtggacgg ttcttcgcaa
     4141 gccagcgacg catcgacata atcggcgtga cgccgcagcc tgccgccagc aacaggaatt
     4201 tatcttctgc tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc cagagataat
     4261 caccgcgttt tacatcgcgc gtcagccact gggagccgac accgtcatca atccgccgca
     4321 cggtcagggt gatatattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg
     4381 tttccgctga gtttcgcacg ctgaccagtg catattgccc ggcgcgatat gggtagtaat
     4441 cgtggcaaat cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct
     4501 gcatccgcca cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgctcaa
     4561 cagttgcttc atgtcttctt caacagtggt gatagaacgc aggccaaatt tctcgttcag
     4621 caccgccagc aggtctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt
     4681 cacgcccaga gaaagcagcg tcagcagaat gacgatcgct ttctgttcaa accaggagag
     4741 caccagcgac agcggcagat cgttgacacc gcagcccagt ttctctgcca gagtgacagc
     4801 cagaataatc gctgagtaag catcgttaca ttgacctgca tctaccagac gcggcagacc
     4861 ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta ccacaggcga gggtcaggat
     4921 caggcagtca tccggcacgc tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc
     4981 gccgtcacag ccaccaagca ggaagatatg acgcagtttt tcacggctca ccagatcaat
     5041 cagcgtatca gcagcgccaa gcagcgtctg gcgaccaaaa cccacggtga tcaggtgcgg
     5101 aatttcgctg tacgggaagc ccgccatttg ttgcgcctgg gcgatgaccg cagaaaaatc
     5161 ttcgccatcc agatgacgca cacccggcca gccaacgatg ctgcgggtcc agatacgatc
     5221 gtcataagcg cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg
     5281 gaaacgagcg aactccactt gctgattctg ccagccactg ccgtagttac cgaccagatg
     5341 cttgaattta cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac
     5401 attaacgccc gtgccttcgg tctgttccag caggttgtag agatctttga gatcgtgacc
     5461 ggaaatcaga atgcatttac ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg
     5521 accgtattta ccggtttcgc ctgcatccag aatgctcatc actttgaagt tcatctggcc
     5581 gatttccatt gaacactcaa gaagcgcgtt catatcggca ggccaggtcc ccagccacgc
     5641 catgatttta tggtactggg cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg
     5701 ttccatatag gccgccgcac ctttcaggcc atacaggcac agcagacgca ggccgagaat
     5761 gttttcgcca atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag
     5821 ctcgccgaga tcgtcgctca ccagttgcag gtcagccatc gggttatcga cgcgcgcgtt
     5881 ggcatctaca gccaggcatt gcgctttcag cgcctcgcgc agggcaatcg cttcacgagc
     5941 gtagccgaca atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaaggcacg
     6001 tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca
     6061 ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga aggtcagaag tttccgccgt
     6121 tttaccacac atcccctgcg cgtatgagca gccgtttcct gccggagtac ggatagtttg
     6181 ttcacattgc acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatgttta
     6241 aggttaagac gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg
     6301 atttagcgca attttggcgg cagggatcta ccgccagaga ggtattacgc agagaaaaag
     6361 gcgatgagga tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc
     6421 atatccagcc cgccagtacg ttgaagaacg ggcagggtga aatccattga tgtggcaccg
     6481 cataagccca gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa cataatagca
     6541 atcagttcac gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat
     6601 tcggtcaata aaataccgga aagagaatac cagccgaaac cggaggccat tgccagcgcg
     6661 gtattgatgg ggagatcaag aataaaggcg ttaattaaac caccaattaa tgaactgaca
     6721 accaccacca cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg
     6781 ccattattgc gcaactgaat accaacgagg aaaagtagca aaattaacgt gtattcactg
     6841 gcttcggtcg cgtgttgtaa gaaagccagt ccactcagac caatggcaaa accaatcact
     6901 actacgccgc acagttttag cgactccagc gccatcgcaa tacgcgacgg gagtttttct
     6961 tgctgatggt ggttgcgcca cggcaggcct cgctccagcc acatcagggc ggcaatatta
     7021 cacagtaaaa taacggtaat actgacggca gaataatgca gaatcgccaa caggttactg
     7081 gcgaggttat cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc
     7141 cagcttaata gctgattaat aacttttaac gcagcttgtt ggcgaagcgg aatgaggtaa
     7201 cccacaatca ggggaaccag aatgattaac agcccagaaa acatgaaaac ccagtccttg
     7261 caaagatgaa gtcgaaatgc gcgatgacac actactgaaa gcggaaggac gagtaaagtt
     7321 gcaattaaaa ggaaatgtta tgcataagga gcagtagagt attcgttttc atttaaagat
     7381 attcttgcgc tttaattaca aactgcaccg atgttggtgg cgtcaaaatc gccgaggcgt
     7441 tccctgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt
     7501 tacctcgcgc ccgacccggt agccgtaagg gataagtcga gggcaccgcg cagcggcgat
     7561 tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ttggccgccc cctgcgcgct
     7621 ccttgcgcca gtggcaatat gtggcttagc tcatgaaagg agcgcaacaa gatgatgaat
     7681 caacatataa caacatctta aaaaaaggcc tgacattacg ccaggccttc tgcgttaatt
     7741 aatcacgctt ttccagcagg gtccggtaaa tcagaccacc gataatgccg ccgacaattg
     7801 gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg
     7861 cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg aatactaatt aagtgaatca
     7921 gggttaaggc cagaccaata gcgatcggcg caaaacctgc cggcgcgaat ttgtcggttg
     7981 cgccgtggat caccaacagg aaacctgcac tcaataccag ttcaactacc agcgcggaaa
     8041 gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg
     8101 ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgct gcaacaatac
     8161 cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccggaaaa cgtccgccag
     8221 cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcaa
     8281 aggccatcgt cagaacggtc agaccgaacg ccaacgccac gccggcaaaa ccaatgccta
     8341 attccgggaa gcctgcggcc agtacagcac taccacagcc accaaaaaca agccagaaag
     8401 taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct
     8461 gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga aataatgcgc
     8521 ggctatttta aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt
     8581 tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagca
     8641 aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg
     8701 ttgtttttgc agacttatca gcaagaggga gtataacgcg attattcgct catttttcag
     8761 acatttgcca tgcttaaatg tgatgtcatc acgtattagc aaggcctttc ccgttatact
     8821 gccagcgtaa aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa
     8881 attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg
     8941 attggggaga acgcgtgggg taaatccagc ttgctggacg ccttaaccct gctgctatcg
     9001 ccagaatcag atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc
     9061 aacgggcgag aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga
     9121 catcgggttc gccgttatcg gccgctggaa gcgtgctgga cgccatgcac cgatggctat
     9181 caccgtattt tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg
     9241 cgcagttttc tcgataaaga cggacatccg attgatgtcg aggatattaa cgatcaggca
     9301 cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt
     9361 attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat
     9421 ttcctcgccc gtgagttgtc ctcacatccg caaaatctct ctgatggaca gattcgtcag
     9481 ggactttccg caatggtgca gttgcttgag cattatttct ctgagcaggg ggctggacag
     9541 gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg gcgctatctg
     9601 gatatcatca accggatgat tgaccgacct ggagggcgct cgtatcgggt tattttgctc
     9661 ggcctgtttg caactttatt gcaggcaaaa ggcacattgc gactggataa agacgcccgt
     9721 ccattgttgc tgattgaaga tccagaaacc cgtttacacc ccattatgct ttcagttgcc
     9781 tggcatctgt tgaatcttct gccattgcag cgcattgcca ccaccaactc gggtgagttg
//
LOCUS       DAHBHC010000126         9804 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHBHC010000126 DAHBHC010000000
VERSION     DAHBHC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.128"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(755..1816)
                     /locus_tag="M2A35_004584"
     CDS             complement(755..1816)
                     /locus_tag="M2A35_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200792.1"
                     /translation="MKLNKTVLAICLVSFNLHAANLVYSANQNDNTVSVVDADKYKKV
                     GSLTLGYPAGDKRLYSPLYNGEINVHGLSYAKQRRELSVVSTVTNSVVRFDTNTGKKI
                     DTIYVGRNPHEPRYTNNEDEIWVTVRGENYISIIDSQTGNEKKRIELESGPGMVTFSH
                     DDKYAYISSSFDEHLWIVDTKTKKVIKKLMMPSSFSPFINTTPDGKEVWVDHKDVGEI
                     TRISTKDNEIIETFKTGKISNHFAFANNKAYVTVGGENSVNIYDYGEKHAKLIKKIQV
                     ETLPHGIWADTKGSKVYFVNELSNTLQIIDADSDKIIAKLPVGALPQALVYAENATDD
                     VDKMIKNISQIPEFRGPKK"
     gene            complement(1803..2171)
                     /locus_tag="M2A35_004585"
     CDS             complement(1803..2171)
                     /locus_tag="M2A35_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCB7200793.1"
                     /translation="MNVKKSHIIFFLLLSQPLGLLYACVDVGNNKADSEVVFKHNMHE
                     TMNKMHNDMLNYKFTGDNDLDFLKMMIPHHQAAIDMSRVILETSESKEVRNLAYSIIT
                     EQNNEINIMNKLVEAKNEIK"
     gene            2593..3403
                     /locus_tag="M2A35_004586"
                     /pseudo
     CDS             2593..3403
                     /locus_tag="M2A35_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582750.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3400..4180
                     /gene="istB"
                     /locus_tag="M2A35_004587"
                     /pseudo
     CDS             3400..4180
                     /gene="istB"
                     /locus_tag="M2A35_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            4604..5770
                     /gene="sopA"
                     /locus_tag="M2A35_004588"
     CDS             4604..5770
                     /gene="sopA"
                     /locus_tag="M2A35_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HCB7200794.1"
                     /translation="MGLMNTLNQCINAGHEMTKAIAIAQFNDDSPDARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRIGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDMALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDATYAIKPTCWPGLNIIPSCLALHRIETELMSKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNCNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            5770..6741
                     /locus_tag="M2A35_004589"
     CDS             5770..6741
                     /locus_tag="M2A35_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HCB7200795.1"
                     /translation="MKRAPVIPKHALNTQPVEDTSSSTPAAPMVDSLIARVGAMARGN
                     AITLPVCGREVKFTLEVLRGDSVEKASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYVRRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGEALQKAFTDKEELLIQQASNLHEQKKIGVIFEAEEIISLLTSVLKTPSASKNSLS
                     SRHQFAPGATALYKGDKMVINLDRSRIPAECIEKIEIILKELEKPGT"
     gene            7263..7514
                     /locus_tag="M2A35_004590"
     CDS             7263..7514
                     /locus_tag="M2A35_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCB7200796.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            7511..7798
                     /locus_tag="M2A35_004591"
     CDS             7511..7798
                     /locus_tag="M2A35_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7200797.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RELKNCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            7934..8056
                     /locus_tag="M2A35_004592"
     CDS             7934..8056
                     /locus_tag="M2A35_004592"
                     /inference="COORDINATES: protein motif:HMM:NF023742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200798.1"
                     /translation="MKRLQAFKFQLRLDDQQEREMRRFVGACRFIFNRSLAASK"
     gene            8097..8240
                     /locus_tag="M2A35_004593"
                     /pseudo
     CDS             8097..8240
                     /locus_tag="M2A35_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095806.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8675..8890
                     /locus_tag="M2A35_004594"
                     /pseudo
     CDS             8675..8890
                     /locus_tag="M2A35_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(9237..9416)
                     /locus_tag="M2A35_004595"
                     /pseudo
     CDS             complement(9237..9416)
                     /locus_tag="M2A35_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         2679 a         2065 c         2185 g         2875 t
ORIGIN      
        1 tttttctcat attttcctgt gaattaatat aacttattta aacgatagct ttaatatatt
       61 tacttttcta cttgtttatt aaatcattga ttaattgatt ttttagacca gagaaactca
      121 ggtgatgtgt cacgtagagt gaatagctgt catgaaagga gataaagtta aatacatccg
      181 atacaagata taagcataca aatttttatg agtgttatta catgtgaaaa ttacaacccc
      241 ctaaagccac tgatatttta gttgattttt tacgattctt tatagtagtg ctggagtaaa
      301 aatcatgagc tatattgatc ttgagtaatt gaggcgatag cctgggtttc tctctcaaaa
      361 taattttctt ttggatctgg ctctgtagtt aatcagaagg ttataagatg ctatccatgg
      421 cgtttattta gtatgtttta tgattatgaa aaagaccatg aaatggtcgg agggattcat
      481 cgatttggtt cttctgattt tttgtatgac atagagtaag atttagcatt ttttagtgtt
      541 ttcttacgct gtaaaaataa aaaaacgtta atggtgtaca ataatgcacc agttatgcaa
      601 tcacatctta tagcaatttt acacaaactg gtgcctctgt ctgttgctgc aaaaatatta
      661 tttcagacaa tatatgaagc agctataagt attaagtata tagaacgtgt catgtttgac
      721 actgaaaaat gatattatta ctgcagtttc ttatttattt tttaggtcct ctaaattcag
      781 gtatttggga aatattttta atcattttat caacatcatc tgttgcattc tctgcataaa
      841 caagagcctg aggcaatgct ccaacaggta attttgctat aatcttatcc gaatcagcat
      901 caataatctg aagtgtattg ctcagttcat ttacaaaata aactttactt cccttggtgt
      961 cagcccagat accatgagga agtgtttcta cttgtatttt ttttataagt tttgcatgct
     1021 tttcaccata atcatatata ttcactgaat tctcgccccc caccgttaca taggccttat
     1081 tgtttgcaaa tgcaaagtga ttactgattt ttcctgtttt gaatgtctcg ataatttcat
     1141 tgtccttagt gctgattctt gtgatctcac caacgtcttt atgatcaacc catacttcct
     1201 ttccatctgg tgttgtattg atgaatggag aaaatgaaga tggcatcatt agttttttta
     1261 tgaccttttt tgtttttgta tcaacaatcc ataagtgctc atcaaaactc gagctaatat
     1321 atgcatattt atcatcatga ctaaaagtga ccattcccgg gcctgattcg agctctatac
     1381 gttttttttc atttcctgtc tgactgtcaa taatcgatat ataattttca cctctgacag
     1441 tcacccatat ttcatcttca ttattagtat atcgaggctc atgtggattt cgtccaacat
     1501 atatagtatc aatttttttt cctgtgtttg tgtcgaatcg tacaacagag tttgtcacag
     1561 ttgaaactac ggagagttcg cgtctttgtt ttgcatatga caggccatgt acatttattt
     1621 ctccattata aagagggcta tataatcgtt tatctcctgc gggataccct aaggttaaac
     1681 taccaacctt tttatattta tcagcatcaa ctacagacac tgtattgtca ttttgatttg
     1741 cagaatatac aagattagct gcatgtaaat taaatgacac taggcatatt gcaagcactg
     1801 tcttatttaa tttcattttt agcctcgacc agtttattca tgatgttaat ttcattattt
     1861 tgctctgtaa ttatggagta agctaaattt cttacttctt tgctttcgga tgtttcaaga
     1921 atgactcgag acatatctat agcagcctgg tgatgaggaa tcatcatttt aaggaagtcc
     1981 agatcgttat cccctgtaaa cttataatta agcatatcat tatgcatttt attcattgtt
     2041 tcatgcatgt tgtgtttaaa aacaacttca gaatctgctt tattgtttcc tacatctaca
     2101 caggcatata ataaacctaa aggttgtgat aaaagtaaaa agaaaatgat atgggatttt
     2161 tttacattca taaatatcac cgtttcgagt ttgtaaaaca ataaaacctg tttatcgtgt
     2221 atctgcgata ttctcttaat tattggaagt ataatttctg agatatatag gtggtgttat
     2281 ttataagttt aatatagtca cactcgcgat tatcaattat tccttttata taacgaataa
     2341 ttttcattat attcttcctc actcgaaaag gaccttacat gacgattaca tttttgtcat
     2401 tttaggatat gattgaatgg ttgcgatata gatataaggc aggaagattt ttctaattag
     2461 cccaaataat actgaatgta tagttgctac ttttgatggg gccgcgtcag cggcatggaa
     2521 ggcggcactt tgttgttcca tatgatacag gagttaaacc gccgaagccc tgcgtaagca
     2581 ggtagcggaa ctgaatatac tccgcgacct gcagttgctt cacaccagga tgaataccgg
     2641 actatattcg ccaacgtatc gtagatgctt attcttacaa agccccggta acggtaatcg
     2701 tttgcgagat cagagatatc gtggtggcat gacaatcatc agggaggtat ttgttcaacc
     2761 ttcaggagcc tgctgctcga aactgagtct agacaacaga tactgactga ctggggcatt
     2821 atgcgtaatg gtcgatcgcc gcttcacgtg ttcgttgcag ttctgggata cagccgaatg
     2881 ccgcacatcg aatttactga caatatgcat gatggtaaac aggaggcctg tcaccataat
     2941 gcgtttcgct tctttggtgc tgcgccgcgc gaagtgttgt atgactatat gaaaacggtg
     3001 gctccgcagc gtgacgcata tcaggctggt cagcactgat tccatccttc gcagtggtac
     3061 tttagcaagg aaatgggcct ctctcccccg acagtgtcgt cttttcagag ttcaaactaa
     3121 aggtaagatg gaacggatgg cgcagttcat ccgtaacggc ttttacatcc cattaatgac
     3181 tcgtctgcgc tggctgcacg atgttgctaa ccaacggaaa catgaaacaa tccagttccc
     3241 tccctgtgat cgctgcttcg aagagcagca atccatgctg gcactgcctc cggagaaaaa
     3301 agagtatggc gcgcatgttg gtgaaaaatc ggtgaacttc gacaaacatt ccctgcatca
     3361 tccactctcc atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat
     3421 caacgactga tggcgctcgc tgggcagttg caactggaaa gccttataag cgctgcgcct
     3481 gcgctgtcac aacaggcagt agaccaggaa tggagttata tggactgcct ggagcatctg
     3541 cttcatgaag aaaaactggc acgtcatcaa cgtaaacagg ctatgtatac ccgaatggca
     3601 gccttcccgg cggtgaaaac gttcgaagag tatgacttca cattcgccac cggagcaccg
     3661 cagaagcaac tcctgtcgtt acgctcactc agcttcatag aacgtaatga aaatatcgta
     3721 ttactggggc catcaggtgt ggggaaaacc catctggcaa tagcgatggg ctatgaagca
     3781 gttcgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcgttatcta
     3841 cggcacaacg tcaggaccgt tataaaacga cgcttcagcg tggagtaatg gcccccgcct
     3901 gctcatcatt gatgaaatag gctatctgcc gttcagtcag gaagaagcaa aacagttctt
     3961 ccaagtcatt gctaaacgtc acgaaaagag cgcaatgatc ctgacatcca atctgccgtt
     4021 cgggcagtgg gatcaaacgt tcgccggtga tgcagcactg acctcagcga tgctggaccg
     4081 tatcttacac cactcacatg tcgttcaaat caaaggcgaa agctatcgac tcagacagaa
     4141 acgaaaggcc ggggttatag cagaagctaa tcctgagtaa aacggtggat caatattggg
     4201 ccgttggtgg agatataagt ggatcacttt tcatccgtcg ttgacagatg tttacggtgg
     4261 ttaccaagga ttattctctc cgatcagtac gcatcgtctg aacgatatag ttccaggaaa
     4321 atggcgatgt tacgttattg aatatatcca ggactgagtg acagccggat atacgctctg
     4381 taaagctgaa ctgagcgcaa gagctatctg tctgaacagc cttccggcta catggctacg
     4441 ataagcgata tcgataatta gtgactgagg cgtgtatgtt cttcttactt cttttttgct
     4501 gtattgctat aactttcgac aactttgcga tttttatgtt gctttgcaat aaattccaag
     4561 atttaataaa aaaatgcaaa gttatgattg aaggatgttc agaatgggac tcatgaatac
     4621 acttaaccag tgcataaacg ctggccatga aatgacgaag gctattgcta ttgcgcagtt
     4681 taatgatgat agcccggacg cgagaaaaat cacccggcgc tggagaattg gtgaagcagc
     4741 agatttagtt ggagtttctt ctcaggccat cagggatgcc gagaaagccg gtcggctgcc
     4801 acatccggat atggaaacac ggggacgggt tgaacagcgt attggttata caattgaaca
     4861 gattaatcac atgcgcgacg tatttggtac gcgactgaga cgtgctgaag atgtatttcc
     4921 gccagtgatt ggggttgctg ctcataaagg tggtgtttat aaaacctctg tttctgttca
     4981 ccttgctcag gatatggctc tgaaagggct acgtgttctg ctcgtggagg gtaatgatcc
     5041 ccagggaaca gcatcaatgt atcacgggtg ggtgcctgat cttcatattc atgcagaaga
     5101 tactctcctg cctttctatc ttggggaaaa ggacgatgcc acttatgcaa taaagcccac
     5161 ttgctggccc gggcttaata ttattccttc ctgtctggct ctgcaccgta ttgaaactga
     5221 attaatgagc aaatttgatg aaggtaaatt acctactgac ccacatctga tgctccgact
     5281 ggccattgaa accgtcgctc atgactatga tgttattgtt attgacagtg cgcctaacct
     5341 ggggattggc acgattaatg tcgtatgtgc tgctgatgtt ctgattgtcc ccacacctgc
     5401 cgaattgttc gattatacct ctgcgctgca atttttcgat atgcttcgtg atctgctcaa
     5461 aaacgtagat cttaaagggt ttgagccaga tgtacgtatt ttgcttacca aatacagtaa
     5521 ttgtaatggc tctcagtctc catggatgga ggagcaaatt cgggatgcct ggggaagcat
     5581 ggttctgaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaaa tccggatgag
     5641 aactgttttt gaacaggcca ttgatcaacg atcttcaact ggtgcctgga gaaatgcact
     5701 ttctatctgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac cacgctggga
     5761 gattagataa tgaagcgtgc acctgtcatt ccaaaacatg ctcttaatac tcaacctgtt
     5821 gaagatactt cgtcatcgac gcctgccgcc ccgatggtgg attcgttaat tgcgcgcgta
     5881 ggggctatgg ctcgtggtaa tgccatcact ttgcctgtat gtggtcggga agtgaaattt
     5941 acccttgaag tacttcgggg agatagtgtt gagaaggcct ctcgggtatg gtctggtaat
     6001 gaacgtgacc aggagttgct tactgaagat gccctggatg atctcatccc ttcttttcta
     6061 ctgaccggtc agcagacacc ggcgttcggt cgaagagtgt ctggtgttat agaaattgcc
     6121 gatgggagtc gtcgtcgtaa agctgctgca cttaccgaga gtgattatcg tgttctggtt
     6181 ggcgaactgg atgacgagca gatggctgca ttgtccagat tgggtaatga ttatcgccca
     6241 acaagtgctt atgaacgtgg ccaacgttat gtaagacgac tgcagaatga gtttgctgga
     6301 aatatttctg cgctggccga tgcggaaaat atttctcgta agattattac ccgctgtatc
     6361 aacacggcta agctgcctaa atcagttgtt gctctttttt ctcatccagg tgagctatca
     6421 gcccggtcag gtgaggcgct tcaaaaggcc tttacagata aagaagagtt acttatacaa
     6481 caggcatcta accttcatga acagaaaaaa ataggggtaa tctttgaagc tgaagaaatc
     6541 atttctcttt taacttctgt gcttaaaacg ccatctgcat caaaaaacag cttaagctca
     6601 cggcatcagt ttgctcccgg ggcaaccgcc ttgtataagg gagacaaaat ggtgattaac
     6661 ctggacaggt ctcgcatacc tgctgagtgt atagagaaaa tagaaattat tcttaaagaa
     6721 cttgaaaagc caggaacctg atacggctgt ctctctttac tttatttcct ttattacagg
     6781 ccaaaaaata taactggcct gaatactctc tcctgaacta cagtttcatc tatattgttg
     6841 gtaaggttac ctgcctggga ccacggtccc agccgtatca ccagtcagct tattagactg
     6901 ggaccatggt cccagtcgta ttattgggca gagtatcagc ctggaactct gcccaataat
     6961 acccgatgct caaattggtc ctcgaatcat cgggaaaata ttgtgattcg tctgttatac
     7021 acggtcgtta gcggtcctga gggtccgtta actcagagat atacctgaat ccttccgggg
     7081 tcactccgac agtgtacaga gacatctgaa cggccgtacc gacttatgct gttttctcct
     7141 ttcggtggtt ccggcttagc ccaccattaa gcacatttgc aaatacatgt ctgaaacaga
     7201 cttgttaacc agtcttttta gacgtatatt taagactctt taaacaatct cgaaggtgtg
     7261 ttatgcctaa tatcattctg tctgacacca gtgcaagtgt cagtgaactg aaaaaaaacc
     7321 caatggcaac agtcggtgcc ggagatggtt atcctgtcgc cattcttaac cgaaaccagc
     7381 cggcattcta ctgtgtgcct gcggaactgt atgaaaggat gcttgatgct ctggatgatc
     7441 aggagctggt gaaactggtt actgaacgca gcaatcagcc actgcatgat gtggacttgg
     7501 atagttattt atgacttata cggtaaaatt cagggatgat gcgctcaaag aatggctgaa
     7561 actggataaa tccattcagc aacagttcgc aaaaaaacta aaaaaatgca gcgaaaatcc
     7621 tcatatacct tcagcaaaac tacgtgaact aaaaaattgc tacaaaatca aacttcgtgc
     7681 atcgggtttt cgtctggtct atcaggttat tgatgacatg ttaattattg cggttgtcgc
     7741 agtaggtaaa cgtgaacgta gtaacgttta taatcttgcc agtgagagaa tgagatagta
     7801 accgagggga taaatactga aaggaagagg ttgataagta atttttatcc tgtgcgttgt
     7861 aaaatcctgt ccttcatggc ggggatataa ggcgcggttt tccgcctgac tttcttttgt
     7921 ttaaaatagc gggatgaaac gactacaggc attcaaattc cagttaagac tagatgatca
     7981 acaggaacga gaaatgaggc gctttgtcgg agcttgtcgt ttcattttta accgatctct
     8041 ggccgcttca aaatgagaat catgaggtcg gtaataaata catcccttac gtaaaaatgg
     8101 ctccctggct ggttgagtgg aaaaatgcca ctgaaacaca atgacttaaa gatgctccgt
     8161 cacagccatt gtaacggtca ctgaaagacc ttgagtgggc cgacaaaaat ttcttccaga
     8221 agcgggctgc tttcccctga ttcaaaagcg gagacagaat gatgcattcc gctacctgca
     8281 gggtgttaag ctcaatcagg aaaacagccg tatttttctg ctgaaacctg gctggatacg
     8341 ctaccggaac agtcgtcaag tcacgggtag ggggggaatg tcactgtcag ccagtcctgc
     8401 tgtaagtggt acatcagcat tcatacaaaa agtgacgtat cgacaccagt tcacccttca
     8461 gcatcaatcg tcgggctaga tgctggcatg gctaaactcg ccacgctgtc agatggcaca
     8521 gtctttgagc ctgtaaacat ttttcagaaa aaccagaaga agctggcgag acttcagcac
     8581 cagtcagtta agccgcaaag ttaaattcag caatagctgg cagaaccaga aacgcaaaat
     8641 acagagactg cattcccgta tcgtaaatat ccgcagcgtg ccggagccag accgggtatg
     8701 gtgcggagat attatgtata tccgggcagg aaatcgctgg tgttatctgg cggtcgtcat
     8761 ggatcttttt tcccgcaagg ttatcggctg ggggctgtca gcgaattctg atactacatt
     8821 gataagcagt gccctgcaga tggcgtatga ggtgcgtggc cagccacagg acgttatgct
     8881 ccatagcgat agtaataatg ccggtatcag tttttatcat cactctgttt gctgtttaac
     8941 cagattggtg tgattgctga tgcagtgaag accttcccgt atcctgactc acacagcgat
     9001 tgcccctttg tgtcctgccc tggacctgtc ggttgccgga agcgccttca tgcgaggcgt
     9061 ctcctcactg atgcgcgtga ctcaagaagg gcctgacggc ttgtctcgtt actgtcctgg
     9121 ccgggttatc tgtctggaga ttcaactctg tttcctcaca ggagctctgt tatggcaggt
     9181 aaagttacgg aaaccactgt tgtggggtaa gcggctcgcc agaaccgtat tgatatttac
     9241 tgagaggtca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggccagtac
     9301 tggatatgct caataacgta acgcagccat gtttctggct ctacattgct cagacggaat
     9361 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctgtcgga acccgcgagt
     9421 cgagtcagcc ggagagaatt tcactccccg gctgctcacg aaaccttacg tgacactctc
     9481 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gttctccccg
     9541 atggtgtaca gagcgctgat gggggcttcc actgccgggg ctcttttact gaccaccaca
     9601 ggataaaaca cactccccga aaacaggagg gggccagatt aagcatctgt gtacgcatga
     9661 gcacacagat gcgggttatg ttcctgtaaa agctacttta atcagtgaaa aaggctgtcg
     9721 ccagtcggcg ttctactttc tccattccgg ctccagccac agggtttcag tgtgacataa
     9781 atgatattcc tctcataatg cctt
//
LOCUS       DAHBHC010000127         9742 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHBHC010000127 DAHBHC010000000
VERSION     DAHBHC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.129"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(60..2066)
                     /gene="mnmC"
                     /locus_tag="M2A35_004596"
     CDS             complement(60..2066)
                     /gene="mnmC"
                     /locus_tag="M2A35_004596"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCB7200799.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            2225..3445
                     /gene="fabB"
                     /locus_tag="M2A35_004597"
     CDS             2225..3445
                     /gene="fabB"
                     /locus_tag="M2A35_004597"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCB7200800.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQATGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            3729..4907
                     /locus_tag="M2A35_004598"
     CDS             3729..4907
                     /locus_tag="M2A35_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCB7200801.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(4904..5899)
                     /gene="flk"
                     /locus_tag="M2A35_004599"
     CDS             complement(4904..5899)
                     /gene="flk"
                     /locus_tag="M2A35_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCB7200802.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(6157..7050)
                     /locus_tag="M2A35_004600"
     CDS             complement(6157..7050)
                     /locus_tag="M2A35_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HCB7200803.1"
                     /translation="MKNMIFSIGALFISISVLPVHAATEHKNFNAVLQCRAIENNKDR
                     LSCYDKSIQPTRTKVAEKFESRDQCPDEKDDDKRLSCYDRFFSPTFTPSVNSKSKTEK
                     LVTTEAQQPNLSEISKCRAENDKETRLNCYDKLFPQDEAVQSESQLEKATDAGKWHTS
                     ITTSPIDDSKNVILSLESDDYIRTPFGEAVTPTLFIACREKKTEVFLSWDVYLGLEQT
                     SMLYRLDKQKAVERNWLISTDTKAVFYKGNDIDFIRKLANSHKMYTKITPYNESPVSA
                     TFNLSGLSNALKPLQDACNWK"
     gene            complement(7055..7387)
                     /locus_tag="M2A35_004601"
     CDS             complement(7055..7387)
                     /locus_tag="M2A35_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCB7200804.1"
                     /translation="MALIKCPECQKEVSDSALCCPACGKQLKKLKRSFFGKLIKWAFI
                     FFNIFMIYTLLVGLGGADEIINNTTSDAEKAGAVIGTGLGLIAIGGLWVIGDIIIGIL
                     VFLTKPKG"
     gene            complement(7904..8731)
                     /locus_tag="M2A35_004602"
     CDS             complement(7904..8731)
                     /locus_tag="M2A35_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCB7200805.1"
                     /translation="MNNKLKVIELNSLDLFRKELLTTIKPEKVEKLLRWYLQSYGGVN
                     FKFGYDRPIFRARKCPNECGYNNISEIYPPPPEKCKIGRMNDDGQAIFYGAYSIGTAL
                     AEINAKEGDYVHIAHFKMPENSESGMRCFAIGEVFNVYHGVNTISIEVFNEIRDIISR
                     IGKDDIRALLSYLYMDALSAELLNSINAHEVNYIYSRIFCRLLLDKYPDVDGLIYPSA
                     KIKGASNIALRTETVKSRMHLVANLVFKVNKIYPYGIVDFSIVKQAKGHTSDGRIVW"
     gene            complement(9147..9287)
                     /locus_tag="M2A35_004603"
     CDS             complement(9147..9287)
                     /locus_tag="M2A35_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCB7200806.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(9478..9738)
                     /locus_tag="M2A35_004604"
     CDS             complement(9478..9738)
                     /locus_tag="M2A35_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCB7200807.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLSQN"
BASE COUNT         2342 a         2395 c         2357 g         2648 t
ORIGIN      
        1 gtcacacgta ggtcggataa ggcgttcacg ccgcatccgg caaacactgc gagagcagat
       61 taccccgcct taaccgcttt acccttcaac aatttccgca cccataaccg attcgggttt
      121 aacgccgcca gcgtactggc atccatcgga atcggttcgt cgctcatctg cgccgccaga
      181 atctcggcac acagcggggc agaacacaaa ccgcgagaac ctaaagccgc aaacataaag
      241 agatcgtcaa aaaccggcgc gcttaccgcc tcatctttct gctccgccaa cgacgcatat
      301 tccacgagtg ttgcctcata atcgggaaca ttgcctacca ttggcagatg atcgcgggtg
      361 gcacaacgca caccgcagcg cgcctcttta tcactgacat caacctcttt tgcccactgt
      421 gcctgcggga aacaatcaat caaccgctgg cgattctgct gctgatcgtc ctcactgtac
      481 gccgtatctt cgctgccgcg atgataactg gcaccaatac aatgatgttg attcgccgga
      541 ttttgtggcg tgagataacc gtcatagcac agcacctgct tcagctctgc cagttccggc
      601 gttgtcggaa tatggctgac ctgcccggca accgaataca ccgggagagt cgacgtttgg
      661 ctgaatcggc tgatttgatg cccgttcgcc agtaccacta cgctgtgtgt tgcttgctga
      721 tctcctgcaa aattcaacaa ccaacagtca tccttacggg ataaattctg taactgatat
      781 tgataataaa tctgcaaacc ctgctgttgc gccagttcca gcacattacg ggtcagttct
      841 gctgggcaca gccaaccacc ttgcggataa gtaatgccgc tgcaatttgt cgcaacgccc
      901 gtaatttgtt caaccgcatt tgcctcaacg gctacagcca gttctgcggg taaatccatt
      961 gacaacatct gtgcgatttt atgctggctt ttctcatccc agcctaactg cgtgacgccg
     1021 caccagtcat gatcaaattt aacgggtaat tggtcgtaaa accgacgagc aaaagtaaac
     1081 gcattagaga aaaagcggtt tagcgcctca tcgtgtttgc ttaataacgg atacagcgcc
     1141 ccctggcgat tgccggaagc acccagtgcg ggggcctcat ccgcgcaata aagcgttacc
     1201 tgccagccgc gccgtaatag cgccagcgac aacaacgcgc tggcaatacc accgccgata
     1261 atcgccgctt cccgtttgct gctgcccgtg cggttaaacc acggcgcgga gcaggggagc
     1321 ggtaatgtct gttccatcac cccgcaaagc atttcccgtt tgcgcccaaa gcccttacgt
     1381 ttttgcatcg tgaatccggc gtcctgcaaa ccgcggcgga caaaaccggc agacgtaaat
     1441 gtcgccagcg tgccgcccgg acgcgccaac cttgccatgg cgttaaacag attttgcgtc
     1501 cacatatccg ggtttttcgc tggcgcaaag ccgtccagaa accaggcatc tactttttga
     1561 tttagcgaat cgtccagttg gctggtcagt tcgttaatat cgccaaacca taaatccagc
     1621 gtcacgcggc cttcatcgag caataaacga tggcaaccgg gcaagggcat tggccactgc
     1681 gcctgaagtt gttctgccca cggagccagt tccggccagt gttgatgcgc taaggctaaa
     1741 tccgcacggg tgaggggaaa tttctcaaaa ctaatgaaat gtaagcgttg taattgcgct
     1801 tgcggatgcg cttcgcgaaa ctgatcaaat gcctgccata gcgtcaggaa gtttaatccg
     1861 gtgccgaagc cgctctctgc taccacaaac agaggatgtg gatgctcagg aaagcgtacc
     1921 tctaattggt tgcctcccag aaaaacataa cgcgtctctt ccagcccgtt atcgttggaa
     1981 aaatagacat cgtcaaaatc tcgggaaaca ggtgtaccct cagcattaaa ttcgaggttg
     2041 gcaggttgta tggagtagtg tttcacgtaa gttactcgtc ttacaggcgg tggctcgatc
     2101 ttagcgatgt gtgtaaggct gcgcaaattt ctctattaaa tggctgatcg gacttgttcg
     2161 gcgtacaagt gtacgctatt gtgcattcga aacttactct atgtgcgact tacagaggta
     2221 ttgaatgaaa cgtgcagtga ttactggcct gggcattgtt tccagcatcg gtaataacca
     2281 gcaggaagtc ctggcatctc tgcgtgaagg acgttcaggg atcactttct ctcaggagct
     2341 gaaggattcc ggcatgcgta gccacgtctg gggcaacgta aaactggata ccactggcct
     2401 cattgaccgc aaagttgtgc gctttatgag cgacgcatcc atttatgcat tcctttctat
     2461 ggagcaggca atcgcagatg cgggcctttc tccggaagct taccagaata acccgcgcgt
     2521 tggcctgatt gcaggttctg gcggcggttc cccgcgtttc caggtgttcg gcgctgacgc
     2581 aatgcgcggc ccgcgcggcc tgaaagcggt tggcccgtat gtggtcacca aagcgatggc
     2641 atccggcgtt tctgcctgcc tcgccacgcc gtttaaaatt catggcgtta actactccat
     2701 cagctccgcg tgtgcgactt ccgcacactg tatcggtaac gcagtagagc agatccaact
     2761 gggcaaacag gacatcgtgt ttgctggcgg cggcgaagag ctgtgctggg aaatggcctg
     2821 cgaattcgac gcgatgggtg cgctgtctac taaatacaac gacaccccgg aaaaagcctc
     2881 ccgtacttac gacgctcacc gtgacggttt cgttatcgct ggcggcggcg gtatggtagt
     2941 ggttgaagag ctggaacacg cgctggcgcg tggtgctcac atctatgctg aaatcgttgg
     3001 ctacggcgca acctctgatg gtgcagacat ggttgctccg tctggcgaag gcgcagtacg
     3061 ctgcatgaag atggcgatgc acggcgttga taccccaatc gattacctga actctcatgg
     3121 tacttcgact ccggttggcg acgtgaaaga actggcagct atccgtgaag tgttcggcga
     3181 taagagcccg gcgatttctg caaccaaagc catgaccggt cactctctgg gcgctgctgg
     3241 cgtacaggaa gctatctact ctctgctgat gctggaacac ggctttatcg ccccgagcat
     3301 caacattgaa gagctggacg agcaggctac gggtctgaac atcgtgaccg aaacgaccga
     3361 tcgcgaactg actaccgtta tgtctaacag cttcggcttc ggcggcacca acgccacgct
     3421 ggtaatgcgc aagctgaaag attaattcgc agtaggtcgg agtagtcgga gtaggtcgga
     3481 taagacgcgc cagcgtcgca tccgacgtta cgcgccaatg cggcctccgg cactaacgca
     3541 aaagggaacc tgatggttcc ctttttcaca tcattgacaa tcgccgccag ttccaggcaa
     3601 acttcccgct ttgtcgattt ccttctgaaa agacgtacgc gttaaatcct gccaacgcac
     3661 tgtaaccctg aaaccagaga gatgagacgg ggatactcct cgccttgcgc tgcattctgg
     3721 agtaatgcat gactgctgta agccaaaccg aaacacgatc ttctgccaat ttttcgctct
     3781 tccgcatcgc ttttgcggtt tttctcacct acatgaccgt agggctgccg ttgccggtta
     3841 tcccgctgtt tgttcatcat gatctgggct atggcaatac catggtcggg attgccgtcg
     3901 ggattcagtt tctggctacg gtgctgacgc gtggctatgc cgggcgactg gccgatcaat
     3961 atggtgcaaa acgttcggcg cttcagggga tgttagcttg tggtctggct ggcggcgcgt
     4021 tgctgctggc ggcgattttg cctgtctccg caccgttcaa atttgccctg ttggtcatcg
     4081 ggcgtttgat tcttgggttt ggtgaaagcc agttactgac aggcgctctg acctgggggt
     4141 taggcatcgt agggccaaaa cactctggca aagtgatgtc atggaatgga atggcgattt
     4201 acggtgccct cgctgttggt gctccgcttg gcctgttgat tcatagccat tacggttttg
     4261 ccgcactggc gatcaccaca atggtattac ccttactggc gtgggcctgt aacggcacag
     4321 tgcgcaaagt accggccctg gcgggagaac gtccatcgct gtggagcgtt gtcgggttta
     4381 tctggaaacc agggttaggg ctggcactac aaggcgttgg ttttgcggtt atcgggactt
     4441 tcgtttcgct ctactttgcc agcaaaggat gggcgatggc gggctttact cttaccgcgt
     4501 ttggcggcgc atttgtcgtg atgcgcgtca tgtttggctg gatgccggac cgttttggcg
     4561 gcgtgaaagt ggcgattgtc tctctgcttg tagaaacggt gggcttgttg ctgctctggc
     4621 aagccccagg tgcgtgggtc gcattagcgg gcgcggcgtt aaccggagcc ggatgttcgc
     4681 ttatctttcc tgcgctgggc gtggaggtgg ttaaacgcgt cccctcacaa gttcgcggca
     4741 ccgcactggg cggttacgcc gcgtttcagg atatcgccct cggcgtctcc gggccgctgg
     4801 cgggaatgct ggcgaccacg tttggttact cttcggtatt tcttgccggg gcgatctctg
     4861 cggtgctggg aattattgtc acgatactgt catttcgtcg gggctaacga accagccaga
     4921 ccagcatcag gataatcgcg actaacagta tccacagcgc gggacgcgtt gccatatttt
     4981 gcaacgtgtc catcatcggt ggaaaaggat taagcagcgg ttgcatatca cgcggatcaa
     5041 tccctttaac ccgccgttga taaagctgat taagaatatc ctcggcctgg gcagaagaaa
     5101 gcgatgattg cgcagaaagg ccatattttt gctggatata tgccgataac gccgccagtt
     5161 cactggcatc taaaggttgt tttagcgcca tctgtagtga ttccagcgtc ggcgtatttt
     5221 gctggcttaa cgtctggcgc gcctgcaacc aggtcaccag atggttaaac agtttcgctg
     5281 gaattaactc gccatctttc accccggaaa gttccagcat cgattgccag atctgtttgc
     5341 tgggttcccc cgttgccgcc gcaagtttgg tcaccagctg ttttagcgca ttgtgctccg
     5401 ccggtaataa aggacggtcg gtcgcctcgc gctgctgtgg ttgcggaata accatcttcc
     5461 cttcctgcaa cagggtgaga atggttttta attgctccgg tgagagctga ttcagcggcg
     5521 tctgaccaaa gttatgacgg atataatccg tgaccgcctg acgattattc cccagacgta
     5581 aatactcccc taattgcgct aaaagctggc gggcagaatg gcttttttgc gcggccagca
     5641 gacgttgcgc cagattatgc tcagcggcag ggaagtgacg cgaaagcagc ggtgaatctc
     5701 ccgacagacc aatatcgtgc ctgatacccg cccacagttc tgctctttgt tgttgcgtca
     5761 gtgaggtcac tttcgtcatt aagctttcca gcgaagtacg ttgctgactg gataaaggct
     5821 gattgcccgc gccagacggc aggttatctc cctgacctgg tggttgccca ggaggagggc
     5881 cggaaatagg ttgtatcatt acgtatcctt atacctgaaa tcttcgcaag tatgcctggc
     5941 cgcgagatta tggcacactt gtccggttaa ctctcgtctc atacaggtaa cacaaacgtg
     6001 aaaatccttg ttgaggaaaa taatagtggt ttaatctttt gatataaaag gatttatatc
     6061 atcgtatgtc cacgcaatga ccacattttc gagttacaga tgacaaaacg cccgttttca
     6121 cgggcgtttt gtcatctgat attggggagg tatatactat ttccagttac aagcatcttg
     6181 aagcggtttt agtgcatttg acaggccgct taaattgaaa gttgcactta cagggctttc
     6241 attatagggg gttatttttg tgtacatttt atgtgagttg gcaagttttc ttatgaaatc
     6301 aatatcatta cctttataaa aaacagcctt tgtatctgta gatattagcc agtttctttc
     6361 aactgctttc tgtttatcaa gacgatacag catgctggtt tgttcaaggc ctaaatatac
     6421 atcccaacta agaaatactt cggttttctt ttctcggcaa gctataaaca gagtgggagt
     6481 aacagcttct ccaaatggag ttctgatata atcatcactt tctaacgata aaattacatt
     6541 tttcgaatca tcaattggcg atgtagtaat ggacgtgtgc cattttcctg catctgtggc
     6601 tttctctaat tgtgattcag attgaacagc ttcatcctgt gggaatagtt tatcgtagca
     6661 gtttagtctg gtttctttat cattttctgc tcggcattta gatatttcag aaagatttgg
     6721 ttgctgagcc tctgttgtta ctagcttttc cgttttagat tttgagttta cagatggagt
     6781 aaatgttgga gaaaaaaaac gatcataaca agataaacgt ttgtcatcat ctttctcatc
     6841 agggcattga tccctgcttt caaatttttc agcaactttc gttcgagtcg gttgtattga
     6901 cttatcgtaa caagaaagtc tgtctttatt gttttctatc gctcggcact ggagcactgc
     6961 attgaaattc ttatgttcag ttgctgcgtg aacaggcagt acactaattg atataaataa
     7021 tgcacctata gaaaaaatca tatttttcat tttattatcc ctttggttta gtaagaaata
     7081 ctaaaattcc gataatgata tcgccaataa cccataagcc tccaatggca attaaaccta
     7141 agcccgtacc aataactgcc ccagcttttt cggcatcgga tgtggtattg tttattattt
     7201 catcagcgcc tcctagtcca actaaaagtg tatagatcat aaaaatatta aaaaatataa
     7261 aagcccattt aatgagtttt ccaaaaaatg aacgcttaag ttttttcagt tgtttaccac
     7321 aagcagggca acataatgct gaatcgctga cctctttctg gcattcagga catttgatta
     7381 atgccatgta atccgtctcc tttggaacac tacaagctga aatttgttac aaaacatttt
     7441 gattttatca gattttttgt tgttgataag tgccaattga ttaactggtt tagagtgata
     7501 catatagaaa ggtagagcag tgtcagtgtg tgatgaaata cccacagttt catgggattt
     7561 ttgcatttag agctgtgaga tagggcaatg tagtttagtt aaggaattga ggttcgatag
     7621 cccgaacctc gcgcagaggc aagatgactg aaagcctgat tccttaatgc acaaaaatga
     7681 gtctctattc atacaagtgt attaactaat cgacatcgcc tgttgtgtgg attgcccccc
     7741 cccataaaca cgggctttct gcttgtttag tagtcacagt atgtctaagc tttctcgtta
     7801 gcttcttctg aaatgacctg cactacaagc ccgcgcttcc catacaccac atgcagtgca
     7861 aactgacgca tcggtttttc aatcagctaa ctaaaagttt ggctcaccaa acgatgcgcc
     7921 catctgatgt atgtcctttg gcttgtttta ctatactgaa atctactatt ccatatggat
     7981 aaattttatt tactttaaac acaagattag caactaaatg cattctcgat ttaactgttt
     8041 ctgtgcgcaa agcgatattt gaggcccctt ttattttagc gctaggataa ataaggccat
     8101 caacatcagg gtatttatca agaagaagac ggcaaaaaat tcttgagtaa atataattta
     8161 cctcatgggc attgatacta ttaagcaact cagcagaaag ggcatccata tacaaatatg
     8221 atagcaaagc tcggatgtcg tccttaccaa ttctgctaat gatgtcgcga atttcattaa
     8281 acacttcaat tgaaattgta tttacaccgt gatacacatt aaagacctca ccgatagcaa
     8341 aacatcgcat tccagactct gaattttccg gcatcttaaa gtgagctatg tgaacataat
     8401 ccccttcttt tgcattaatt tcggctaaag ctgttccgat actataagcg ccatagaaga
     8461 tagcttgtcc atcatcattc attcggccta ttttacattt ttctggtgga gggggatata
     8521 tctcactgat gttattatat ccgcattcat ttgggcactt tcgcgctcta aaaattggtc
     8581 tgtcataacc gaacttgaaa ttaacaccgc catatgattg aagataccat ctaagtaatt
     8641 tctctacttt ctctggcttt atagtagtta gtaattcttt acgaaacaga tcaagactat
     8701 ttaactcaat tacctttagc ttattattca tgaccattcc tataatttta gagcttcgtt
     8761 attcacttta cacttcatgc tgaccagtta ccgccactga gtctactgca caggctaaca
     8821 atttatggtt atttttcaat gtacaagtac aaaaaatcaa ggcgttctag aactagaagt
     8881 cgtcagttga ccgcgaaagg taagaggcgg cattcagaca atgcggggga tacaccgcca
     8941 atgtcgagta gttaagcgct tttatgaata gagactcatc ttgggcatta aggaatcagg
     9001 ctttcggtca tcttgcctct gcgcgaggtt cgggctatcg aacctcaatt ccttaactaa
     9061 ctgaacggca ttggtgagat agggtgcctt atcgacctta ccctggcaac cgattgacgg
     9121 gggattgctc ccccgtcgcg gtttccttac tgcttacact gtaagaacgc cgcaaactcc
     9181 gctccccaga agctcatccg tatttcgcac agcgaaccgt gcagcatcca gatgatgagg
     9241 attgccgtca cgcagaacgt gatggccgta agcgattttt gcgacatagc gcttgctcct
     9301 ttttcaggga ggcgctaacc tatcacttgc taaggttaga cggttagggc ctcggttaaa
     9361 cagaaatgtt ttccggggcc tttccacatc tggccttcgg gtattccctc cgaccatcag
     9421 ccgaaaggca cccgcgcgta atctatcgct tttttgttac tccggcaatt ctgcctgtta
     9481 attctgagat aaaggcaaac tcatctgatt gtttcccctg tgtgaagctg gcagctcatg
     9541 ccatgggata ccttctgatg agtgaacgcc ggaggcgtgt ttcgatgtga atttatggaa
     9601 agcttccagt gttgagaagc atacgccgca ttccaggttg ttacactggt aatacttttg
     9661 ccgcacggtg tttgaatcat tttccggacg actggtgcgg atacgggcag atgcgccaca
     9721 aagcggacaa cggaacatag cg
//
LOCUS       DAHBHC010000128         9646 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHBHC010000128 DAHBHC010000000
VERSION     DAHBHC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.130"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..242)
                     /locus_tag="M2A35_004605"
     CDS             complement(<1..242)
                     /locus_tag="M2A35_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200808.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFEL"
     gene            complement(1063..1275)
                     /locus_tag="M2A35_004606"
     CDS             complement(1063..1275)
                     /locus_tag="M2A35_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCB7200809.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(1351..1968)
                     /locus_tag="M2A35_004607"
     CDS             complement(1351..1968)
                     /locus_tag="M2A35_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCB7200810.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2235..3734
                     /gene="ansP"
                     /locus_tag="M2A35_004608"
     CDS             2235..3734
                     /gene="ansP"
                     /locus_tag="M2A35_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCB7200811.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3847..4908)
                     /locus_tag="M2A35_004609"
     CDS             complement(3847..4908)
                     /locus_tag="M2A35_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCB7200812.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5150..7252
                     /locus_tag="M2A35_004610"
     CDS             5150..7252
                     /locus_tag="M2A35_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCB7200813.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7288..7953)
                     /gene="mcbR"
                     /locus_tag="M2A35_004611"
     CDS             complement(7288..7953)
                     /gene="mcbR"
                     /locus_tag="M2A35_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCB7200814.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(8151..9188)
                     /gene="curA"
                     /locus_tag="M2A35_004612"
     CDS             complement(8151..9188)
                     /gene="curA"
                     /locus_tag="M2A35_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCB7200815.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            9372..>9646
                     /locus_tag="M2A35_004613"
     CDS             9372..>9646
                     /locus_tag="M2A35_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7200816.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDH"
BASE COUNT         2447 a         2326 c         2284 g         2589 t
ORIGIN      
        1 agttcaaacc aggtcaccac acctttcacc cggcgctgta cgtcatcgcc ctgccatacc
       61 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggaaagaaa ctgctgactg
      121 accagagaaa ggtcgaggga aaaaagtgaa gagagtgact gggtgagatg aaaggaaacc
      181 accgcaaacg cgtccggtgg caggccgtcc acttcaagtg tgaaacgtaa tccggttgac
      241 atagcaacct ccgtgaaaat gaataataat ccgcacatat tgcatgtaca acatgtcggg
      301 cggatgagat aagcgagtcg acatctggcg ttaccgggga atatcagaaa aatgtcatgg
      361 ttatgtgatg atttagtctg ggattttact tcagagaaac gggtgtctgt ttagtcaaca
      421 aaataatcac aaacgcaacg gatgggttct cgacgttatt cacgacaggc tcgtacatta
      481 cagcgagtaa taaaaaacct gatatgcaaa gtcaaaccaa tattcataat gtctttttat
      541 tcacaacaat aaataattca ataacaatca ttgaaatgaa tgatgatttg tataaggaat
      601 atacgctacc gccccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
      661 cactggctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatatc
      721 ataagcaaat agcatagaaa ataatgacaa ttcccccgag gggaccattt ccaaagcact
      781 gcaccaccac ctggtgcccg gatgatcttg cttcagtggt aagagtacct gattcattgt
      841 tcccaaaatg atgttttccc cggccccgcc aataatgtgc agctctcacg tgataatcta
      901 ttgattaggt tatattaaga atcaacattt aaaatacatc cattttcttg aataaatcaa
      961 catgtttatt aatcaattgc tgttttagct ctgactcatc aagctctcca tatttgtcct
     1021 acagataatt gcgaataaat acagagaacg ccatttatcc ttttagcata cctgccggat
     1081 atttttattt gtattaatat taattgcatc tccaactccg agcatatcca tcattaaaaa
     1141 ctttttgaga ttatagaata cctgtgataa caagtgaaac atctgcacaa tgtgaatagt
     1201 tagtttcttt tcagaggaaa tgaaatagac aagctgaatg aaggggagag tgatataaat
     1261 caaaaaacat aacatgaatt taattaatta cgcccatcat tgtggataaa gttactgaat
     1321 aataaataac ataatcctct ggtatcccac tcaaataatt tcattccttt ttaatacttc
     1381 ttgtaatttc ggtaactgac atacagcatc agcaatcgca ctgatattcg tggcattatc
     1441 ctgaaaccat tcatgcccag gcccccatgt gcgcatagtg caaagataac agtccaccag
     1501 cgttagttgc tcaccaaacg cataaggttc agcggtcaat tgtgaattca gccagatata
     1561 aagcgattta cgatattcaa taacattctt ctttaactgt tcaggtgcgt caggagccca
     1621 acgctcaggg taatcggcga aagtgaatgt tggatagaca ttagcaacca gccagaccaa
     1681 tagccgttga aactgctgac gttcggcgcg cccaaccggt ggcgcaaggt ccgggcgacg
     1741 atcgagaacc atcaatgcaa tcgccgctgt ctccgtcata atctcgtcat tttccagcgc
     1801 cagtgtcgga atctgacaca acgggttcag ggttttcaat aactcgcgtg aagttccctc
     1861 gtggtcaaaa ccactgacat caacgaactg ataaggaata tcagccagtg ttagcatcag
     1921 ctcactgatt gtagagcccc atccgggtac gccatacacc ttaatcatat gcccctcaca
     1981 atcttttaaa gattaagtgt aggacagtac acaggttaac cagcaaaagg atttgtcatt
     2041 ttttcgtcag ttgtattaat ccccttcagg attgtaaggg gatgttttgc atcgtttatc
     2101 gataacgggt aggatgctgc gccgatagtt ttatacccat aaagaataat ggtgataact
     2161 atcatcgcca ggatgaataa acattgttca tggcaactta tatgactttt tcattaaagc
     2221 aatcagggag agcaatgagt aaacacgaca ccgacacttc agatcaacac gccgcgaaac
     2281 gccgctggct taatgcccac gaagaggggt atcacaaagc gatgggcaat cgccaggtac
     2341 agatgatcgc cattggcggc gcgattggca ccggcttgtt tttaggtgca ggagcccgac
     2401 tgcaaatggc gggtccagca ctggcactgg tttatttaat ttgtggcttg ttttcgtttt
     2461 ttattctgcg tgcattgggt gagctggtgc tacaccgccc ttccagtggc agttttgtct
     2521 cttatgcccg tgagtttttg ggtgagaaag ccgcttatgt tgctggctgg atgtacttca
     2581 tcaactgggc gatgacgggg attgttgata ttaccgccgt cgctctgtat atgcattact
     2641 ggggtgcgtt tggcggcgtg ccgcagtggg tctttgcgct cgctgcgctg accatcgttg
     2701 gcaccatgaa tatgatcggt gtgaaatggt ttgcggagat ggagttctgg tttgcgctta
     2761 ttaaagtgct cgccattgtg acctttttgg tcgtgggtac agtgttcctc ggtagcggtc
     2821 agccgctgga tggcaacacc actggctttc atttaatcac cgataatggc ggcttcttcc
     2881 cccacggttt gctgcctgcg ctggtgttga ttcagggcgt agtgtttgct tttgcctcca
     2941 ttgaaatggt gggtacagct gccggagaat gtaaagatcc gcagaccatg gtgcctaaag
     3001 cgattaacag tgtgatttgg cgtattggcc tgttttacgt cggctccgtg gtgttgctgg
     3061 ttatgttatt gccgtggagc gcgtatcagg cggggcaaag tccgttcgtg acgtttttct
     3121 ctaaactggg tgtgccatat atcggcagca ttatgaacat tgtggtgctg accgctgccc
     3181 tctccagcct gaactcaggc ctgtactgca ccgggcgtat tctgcgctcg atggcgatgg
     3241 gcggttccgc accgagtttt atggcgaaaa tgagtcgtca gcatgtgccg tatgccggga
     3301 ttctggcgac actggttgtg tatgtcgtcg gcgtattcct caactatctg gtgccgtcgc
     3361 gcgtatttga gattgtgttg aacttcgcgt cgctgggaat catcgcttca tgggcgttta
     3421 tcatcgtgtg ccagatgcgc ctgcgtaaag caattaaaca aggcaaagca gcggatgtca
     3481 gttttaaact gcctggcgcg cccttcactt cctggctgac attactgttt ttactgagtg
     3541 tcctggtgct gatggcgttc gattacccga acgggactta tactatcgcg gcgctgccga
     3601 ttatcggtat tctgctggtt ataggctggt ttggtgtgcg caaacgcgtt gctgaaattc
     3661 acagcactgc gccagtcgtc gaggaagatg aagaaaaaca ggaaattgtg tttaagcctg
     3721 aaacggcgag ttaattgcaa aattgcccgg ctacgtgccg ggcaaattca gattactgac
     3781 gaaggtgccg gatgcggcgt gataaaagtc aggcaagtct ctccgttttc accctcgcca
     3841 tatctattac agcgcaatac gaatcacatc gtctggctgg gtagcttcct gctgtttagt
     3901 ggatttttgt ttcacactga catacagcgt tttgccatcg gcagacagcg ccaggctgtt
     3961 tggatgagtc ggcgtatcga acgttttcac cactttatag cttttcgcgt caatcacact
     4021 aactttacct gcctgacgat gcgttacgta ggcttcatta cgcgctgggt taaacagcac
     4081 agccagtgat tccggtgccg caaccttcgc cagaatattg ccattacggg tatcgaccac
     4141 taacacttct gcggctttag aatcggtgat aaatgcacgc tgcctggcgg tatcaaggct
     4201 aatgttgata aagaagtgct ctttgccgtc atccagcagc tttttacggc tgaggatttt
     4261 attgtcggcg gtgtcgatgg taatcaattc gccgtcagcg ttagtggtgt aaagacgttt
     4321 gcctttgcta tccagcgcca gaccggtact cattttaccg gtgttctgga tggcggtttt
     4381 cagtttgata ttctcgccat caacgaccca aatcacgcta tctttaccaa taccactgat
     4441 gtaaacggtg ttcgtggcat catcagctac cagctcacgc ggttgcagcg ggcgcacctc
     4501 ttccgtgcgc ttacgatcat ccagcaccag acggcctttc acttcgcccg ttttggcatc
     4561 tatcgccgtg accgcgctgt ttacggtgtt accaaaccac aacgtctgag tcgtgttatt
     4621 gatggtggca ccaaacggct tgagatcgtt atggatcgcc tgcgtcactt ccagagtgac
     4681 cggatcaaga cgataaacca cgccaccttt atccagtttg cggctttgcg aagtggcgag
     4741 ccacagcgcg ttttcttgct ggctataagc catttcgtag gcacctttac ctaccgcttt
     4801 acgcagcatt tcttctgcgg cctgcgtact gaatgatgaa gcaacaagca atgaacctaa
     4861 cagtaatgaa ccacgcaggc gcgatgaaaa cagatgacgt aaatgcatga cgactccctt
     4921 tgatgaacca atatttttgc tgcttcacaa tgcttcgcta agcgtaatca tcgttacgct
     4981 cttgttataa atgggaatgg taatcattat tttctcaaat gtggagcatt tcttgccgtt
     5041 acgactacaa ttttctcaat taattaacgt atcgtgccgt ttcgttttcc ataaatttac
     5101 aattttatta ccatgacagc gttttcccat tcttaacctg gcttttttca tgaagatttt
     5161 ttccgtccga cagaccgttt tgcccgcact gcttgtcctt tcccccgttg tttttgccgc
     5221 tgatgaacag actatgattg tcagtgccgc accgcaggtg gtttcagaac tggatacccc
     5281 agcagcagta agcgtggtgg atggcgagga gatgcgcctg gcaacaccgc gcattaactt
     5341 gtccgaatca ctgaccggcg tgcctggttt gcaggtacaa aaccggcaga actatgcgca
     5401 agatttacag ctgtcgattc gcggatttgg ctcccgctcc acttacggta ttcgcggtat
     5461 tcgcctgtat gtggacggta ttcccgccac catgcccgac gggcaagggc aaacatccaa
     5521 catcgattta agcagtgtgc aaaatgtgga agtgctgcgt ggccccttct ctgccctgta
     5581 tggcaacgcg tctggtgggg taatgaatgt caccacccag accggacaac agccaccaac
     5641 cattgaagcc agtagttact acggcagttt tggcagctgg cgctatgggc tgaaagcaac
     5701 gggcgcaacg ggagacggca cacagcctgg cgatgtcgat tacaccgtct caaccacgcg
     5761 ttttacgacc cacggctatc gtgaccatag tggcgcacag aaaaatttag ctaatgccaa
     5821 actgggcgta cgcattgatg aagccagcaa attaagtctg attttcaata gtgtggatat
     5881 caaagcagat gacccaggtg ggctaaccaa agcagaatgg aaggctaatc cacaacaagc
     5941 gcctcgtgca gaacagtacg atacgcgaaa aaccatcaag caaactcagg ctgggttgcg
     6001 ctatgagcgt agcctgagcg cgcaagatga tatgagtgtg atgatgtatg ccggagagcg
     6061 agaaacgacc cagtaccagt caatacccat ggcaccacaa cttaacccgt cacatgcggg
     6121 cggcgtgatt accctgcaac gccattacca ggggatagac agccgctgga cacaccgtgg
     6181 tgaactgggc gttccggtca cgttcactac cggcctgaac tacgaaaaca tgagtgaaaa
     6241 ccgcaagggc tacaataact tccgcctgaa tagcggcatg ccggagtacg ggcaaaaagg
     6301 tgagttgcgt cgcgacgaac gcaatctgat gtggaacatc gatccctatt tacagacgca
     6361 gtggcagctg agcgaaaaac tgtcgctgga tgctggcgtg cgctacagct ccgtgtggtt
     6421 tgattccaac gaccattacg ttactccggg taacggcgat gacagcggtg atgccagtta
     6481 tcataaatgg ctacctgccg gttcgttaaa atatgcaatg accgatgcct ggaatatcta
     6541 tctggcagcc gggcgaggtt ttgaaacgcc gacgattaat gagctgtctt atcgtgctga
     6601 tgggcaaagc ggtatgaact ttggtttaaa accatccacc aacgatacaa ttgagatcgg
     6661 cagtaaaacg cgtattggtg atgggctgct tagtctcgca ttgtttcaga ccgacactga
     6721 tgatgaaatt gttgtcgata gcagtagcgg tgggcgtacg acttacaaaa atgccggaaa
     6781 gacccgtcgt caaggcgctg aactggcatg ggatcaacgt ttcgcaggag attttcgcgt
     6841 aaacgcgtcc tggacctggc ttgatgcgac ctatcgcagc aatgtttgca atgaacagga
     6901 ttgtaacggt aatcggatgc cagggatcgc ccgtaatatg ggctttgcgt cgataggtta
     6961 tgtaccggaa gatggttggt atgcaggcac ggaagcgcgt tatatgggcg atattatggc
     7021 agatgatgaa aatacggcaa aagcgccgtc ttatactctc gtcggcttat tcaccgggta
     7081 taaatacaat taccacaatt taactgtgga tttatttggt cgtgtcgata atttattcga
     7141 taaagaatac gttggttctg tcattgtcaa tgagtcaaac gggcgatatt acgaaccttc
     7201 gcccggacga aattatggtg tcggcatgaa tattgcgtgg agatttgagt aattaccttg
     7261 atgcccggta tttgccgggc atttacttta acgattgtat tgctggtata aaatagcaat
     7321 attttgttgc ataatttcag caagacaatg tctgcatccc tctttgtctt ttgcttttaa
     7381 cgcagcgagt aacagatgat agttttctat atgctcccgt aattccgctg gattaatcgc
     7441 ttcatagaga taatgtaaac caggccccat cctgacccac agttgctcaa tcatctcaca
     7501 caggatgggc atatttgagc gatgataaat cgctaagcga aatagcctgt ttacattaat
     7561 gatttgttcc atgtcgccct tttcttgcgc ctgctgtaat ttctcgagca attcctggag
     7621 ttccgccagg tcttgcgggg tgaggttttc aacagccaga gcaactgcca ttaattccag
     7681 ttcgtagcgg atccgattga tttcgtctaa ttgacgtttc ctcacttccg gaactgtaaa
     7741 tgcttgtgca ggtgcgacag aaagcgcatt caccgaaacc agacgtaata atgcttcacg
     7801 cacaggtgta atactcatac ctaattgctc cgccagattt ttagtaatca ggcgtgcgcc
     7861 aggttttaat gcgccaatac taagctgatg tttcaggtcg ttctcaacct gcagagtcaa
     7921 actgacatgt ttcatttttc ccattccagg cattttaatt ccttcgcgtg ttttctgtcc
     7981 aaccctgcct gcacggcaga aggagttcac ctggtgtatt aaagtgaata atcataaacc
     8041 acccaccttt gttgttatta gcagcagaac aatgacgtgg ttagttaatt aaagctgaaa
     8101 ataagctttt tgcctggatt tttatggcgg cttacgccgc cgttgaaact ttaatcatca
     8161 cccgcgacgc ggatcaccac tttgccgaag tttttaccct tcagcaggcc gataaacgtc
     8221 tgtggcgcat tctctaaacc atcagtaata tcttcgtggt agtggatttt atcctctttc
     8281 acccattgcc ccatctcctt ctgaaactca tggatgcggt gaccataatc ctgagcgata
     8341 ataaaccctt gcaagcgaat acgttttttc agcaccgttg ccatcaacag gggtaaacga
     8401 tccggaccgg gtggtagctc tgtagcgtta tagctgctca ctaagccaca aacgggaatg
     8461 cgcgcagatg tattaagtaa tggtagcact gcatcaaata ccttaccgcc cacgttttca
     8521 taatagatat caataccttt tgggcacgct ttcaccagtt gttcggcaaa atcatccgcg
     8581 tggtgatcaa gacaaacatc gaagcccagc acctcgatag catggcggca tttttccgcg
     8641 ccaccggcaa cccccaccac cctgcaacct ttaagtttgc cgatttgccc caccgttgct
     8701 cccacaggac ctgtcgccgc agctaccacc aacgtttcgc cctctttagg ctgaccgata
     8761 tccagcagcc ccatataagc ggtaaagcct ggcatcccca gcacacccag cgaccacgat
     8821 ggattttgcg gatgatcgcc aagtttcacc agatcatcac cactggatat gtcatagtcc
     8881 tgccatccac tgtagcccag cacccagtcg ccaggctgat aatcaggatg attcgactcc
     8941 acaacacggc tcaccgtgcc gccgaccatc acgccgccaa tatcaacagg cggtgaataa
     9001 gatggctcat cgctcatacg tccacgcata tacgggtcca gggacaaata aactgtgcgc
     9061 agtaacacct gtccttcacc cggtgtggcg acatcatctt cttcaagacg gaaattctcc
     9121 ggaaccggtg cgccatgtgg acgcgaggcc agaacccaac ggcggttacg ctgcttttgt
     9181 tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc aggctcgcag
     9241 gattggtttt gtttgcagcc gtcacatccc ctctgcaaca cgcaataatt tcatcgagga
     9301 atccgttgct tcgggctgtt ttcgatagta ttgataacat catcatcatc attattttat
     9361 tgaggccaga catgtccatc cgttttgccc gcaaagccga ctgtgctgcc attgcggaaa
     9421 tttataacca cgccgtgttg tatacggcgg ctatctggaa tgaccaaacg gtggatgcag
     9481 ataatcgcat tgcctggttt gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg
     9541 aagaagatag cgtagtgacg ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt
     9601 ttcgccatac cgtggaacat tcggtttatg tccatcccga tcatca
//
LOCUS       DAHBHC010000129         9492 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHBHC010000129 DAHBHC010000000
VERSION     DAHBHC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.131"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(63..887)
                     /locus_tag="M2A35_004614"
     CDS             complement(63..887)
                     /locus_tag="M2A35_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200817.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            complement(887..2236)
                     /locus_tag="M2A35_004615"
     CDS             complement(887..2236)
                     /locus_tag="M2A35_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCB7200818.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(2282..3040)
                     /locus_tag="M2A35_004616"
     CDS             complement(2282..3040)
                     /locus_tag="M2A35_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200819.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(3072..3785)
                     /locus_tag="M2A35_004617"
     CDS             complement(3072..3785)
                     /locus_tag="M2A35_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCB7200820.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3959..4651
                     /locus_tag="M2A35_004618"
     CDS             3959..4651
                     /locus_tag="M2A35_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCB7200821.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEK
                     AITDFMTARGYH"
     gene            complement(4700..6199)
                     /gene="pitB"
                     /locus_tag="M2A35_004619"
     CDS             complement(4700..6199)
                     /gene="pitB"
                     /locus_tag="M2A35_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCB7200822.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(6491..8350)
                     /gene="gss"
                     /locus_tag="M2A35_004620"
     CDS             complement(6491..8350)
                     /gene="gss"
                     /locus_tag="M2A35_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCB7200823.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PYHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            8555..9421
                     /gene="yghU"
                     /locus_tag="M2A35_004621"
     CDS             8555..9421
                     /gene="yghU"
                     /locus_tag="M2A35_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCB7200824.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT         2459 a         2467 c         2298 g         2268 t
ORIGIN      
        1 acccggggct aagagcatga taatcaacgc aataatcaaa cgagatgaca tcattgctcc
       61 ggctatagcg gaaaacgcga aatcagcagc tctaagcgct gacgaatatc gctatccagc
      121 gccaacggtg catcatacga ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt
      181 ttatctccca tcaggccatt aatggcggcg aggaaacggg aaatctgtaa cgcctcttta
      241 ttttcaggat caagcgatct taaggtgtcg taataagaaa cctgttggct gatgattttt
      301 cgcacatcat gaaattccct gccggttagt cgcggtttgg caataatctc tttcagggta
      361 tgcatttgct tttgccgata ggcaataaaa ctgcttacgc tttctaactg gctatggcgt
      421 aatgaatagt tcaccaacga ccagaccgga gagcttaaat aaatccgtaa caggttgccg
      481 taatacgaca caatattttt cttaccgtta cgaaagccgt cctgaaaacg cccaagaaaa
      541 acggtcgttt tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca
      601 aaacgcaggt gcttgtagcg cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc
      661 agctcatcgc ctttggcttg tttgcgaact aaacgcagta attcctgacg atcaacgcca
      721 ttgagccaga attgcaggca taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt
      781 agtgcaatag tcggcggcaa actaacctgt gcatcaacga catcatcttc ttcaatcgca
      841 acaaatagac gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc
      901 cggctgcgtt ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg
      961 agagcgccac gtccagacat tgcccaccga agtaagcaaa cgctgctccc agcacaccaa
     1021 acagatgaca gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca
     1081 cgagaaaagc gcgctgtttt cccgccatca atagcaatga ctcctgaggg aagcccagca
     1141 tcgaaatcac aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa
     1201 cgccaaacac cagcgaaatg agcggcttgc cgacgataaa caccgcgagc gccaccagaa
     1261 taccgattcc gcccgccagt aaaccggact tcacacctaa caaccacggt ctggtggtgc
     1321 gcggatctaa acgcatcacc tctgggtaaa aacttttacc cagcaaaccc gctggcgttc
     1381 cggcggcgtc gaagaatgtc atggcgattt taaataaccc ggcggcagcg ggtcctaaca
     1441 cgatccccac cagcactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt
     1501 tggttgacca gacaaaactc cacgcgcctt tgatgtgtcg ggcagactca aacagattca
     1561 atttgaaggc attatggata tttctgcggc gtaattcgcg cgctgcaaac caccagtaca
     1621 tggtgccgcc aaccagattc gacacgtacc aggcaataac aaaacccgca aaaccaaagt
     1681 caaaatacca ggctacgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta
     1741 cagcaattaa atcgaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca
     1801 ttgaaggaat gagcgtgcaa tagagcgccg ccagccaaaa gctttggtca tccagaccta
     1861 atgaatggga aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga
     1921 cgatatccag cgagaaggaa aatgagacga cattgcgaaa ttgctgcgga ttattgttgg
     1981 tcaaggctgg cgttccgtac tgaaccacca gttgccatgt ctgaaactta ataaaatcgc
     2041 tgatcgactt ggcgtacgat tgcacaatca ccagtacgcc aaacatggca ggcgtcatcc
     2101 ctttaccggc acacgagagc gccaacagac ccagcaaggc gctcacgaca ttactggagc
     2161 ctaaccaggc gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga
     2221 tgttaaaacc cgccaattca gcctgacctt atcgttgata gtaaaaagta tcccgccagc
     2281 cttaagttaa acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtgcct
     2341 gttccagcac ttcgctgtag ggcgctctgg aatcaatctc aagaattttt gtgccgttat
     2401 agccaatcgt cgacatgacg ccgattttgt cctgcagctc ggcatagtca tggtcaggct
     2461 tgcgggagat ggcagtctca atatcaatgc ccaggcgaat aattaattcc gggcgatatt
     2521 gcgccatttg ttggtataaa cgccgttcgc gctgcgccag aaacatgctg attttcccgg
     2581 tcgcacgttc gacgccaatc cccggtccat cataataaaa gcccgaaatt tcagcctgcg
     2641 ggaagcgatc gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgtagat
     2701 tcgccattct tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt
     2761 tggttttcat gctttgggtt ttcgaggatt tggccgccag tcgccgttcc agccagacgc
     2821 caaccaacgg caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc
     2881 gccgctcggt ttgccagtgt tgttgcagcg atttcaccag gtcggtggtc agtgtggatt
     2941 taccggtgcc atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg
     3001 cggttgtact attaacagtt tgagtttgta gtgcatccat tcgttaaatc atcccctatc
     3061 cattttggtg atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cagaatttca
     3121 tcggcggggt gtcgcccatc cagttcaagg atctttgcgc cattgaatgt cagttgcggc
     3181 gtaacggcga ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg
     3241 gtttgttcat caatgccaag acgaatcaac aatacgggca aataagatgc catccattgg
     3301 tacagcttca gctcgcgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt
     3361 gccaattgcg ggccatcaaa gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg
     3421 atgagcagaa agccttgctg gcttttacac aacattttgc gaaacttgta cgcccgccag
     3481 caggaaagca gatagataac cagcgcagta atattgccag gcggtgttga gggtttttcg
     3541 tgcacatgtg ccgctttact tcgcagataa cgcccacaag gtgcgccaat aacagggagc
     3601 tgtgaaatcc attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc
     3661 attcttgctg ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca
     3721 catcctacaa tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga
     3781 ttcacgaaaa acccggcagt aaataatgta ttgaatatta cgtggtccgt gctcagaata
     3841 tccgttcaac cttattgtga caatggttaa ttgtgacatt gacctgaatt acgctttcat
     3901 aaaaacatat taaccaaata aatattttta atggatattt aaattaaagg atatattcat
     3961 gcagtcaata acacctccat taattgccgt tattggtagc gatggttcag gcaagtcaac
     4021 ggtgtgtgaa catcttatta ccgttgtcga aaaatatggt gctgccgaaa gagttcattt
     4081 aggaaaacag gccggaaatg tcggtcgtgc agtgacaaaa ttaccgttga tgggaaaatc
     4141 cttacataaa acaattgaac gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt
     4201 tccggcgctg gtaattacag cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct
     4261 tgcctgtcgt cgtcgcgggt taattgttct aaccgaccgt tatcctcagg accaaattcc
     4321 tggcgcttac gatggtacgg tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg
     4381 gctggcaagc caggaacgta aagcgtttca ctggatggcg agccataagc ctgatctggt
     4441 catcaaactc aatgttgacc ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga
     4501 atcgctggcg aggaagattg ccataacgcc acagttaacc tttggtggtg cacaactggt
     4561 tgatatcgat gccaatcagc cactggaaca ggtgttggtt gatgtagaaa aagcgattac
     4621 ggattttatg accgcgcgtg gttatcacta gtcaaaaatg gaaatgcccg atcgccagga
     4681 ccgggcattt tcaggaaggt taaatcaact gcaatgctat ccagtacagc ccaccagaaa
     4741 gaaaaattgc cgccggtaaa gtaaacaccc acgccatcag gatgctggtt acagttttac
     4801 gctgtaaccc accgccgtcc accaccatcg tccctgctac cgcagacgag aggacgtgtg
     4861 ttgtggagac aggcatccca atataactgg caaggccgat agacactgcc gccgtcattt
     4921 gtgccgccat gccttgcgca tacgtcatgc cgcgcttacc aatcttctca ccgatggtca
     4981 tcgctacacg acgccagcca atcatggtgc caatgccgag cgccagtgct accgccatga
     5041 tgatccacac cggagcgtac tcaatggtgc ttaacatatc gctgcgcagt tttttcagca
     5101 ggttctggtc ttctttactg acgcctggca gtttcgccag cttcgcggag gtatcagaga
     5161 tgcacagcat aatgcggcgc agctggctgc gctgactcac ggttaacggc tcgtaacttt
     5221 ccatattgcc tggcagcatc gttttaacgc gcgcaatagc atcaaaggta tttgccggat
     5281 gacagtgaaa ctctgttact tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca
     5341 tcgcaatcaa cttctgcggc agttcaggat gctgttgcag gtagtgttcg aagttggtaa
     5401 cggcatcgcg ggtacgggta atttcatagc cggacgcatt catattgacg acgaagccag
     5461 caggggcaat ccccaccagt accagcatta ccaggccgat ccctttctgt ccgtcgttcg
     5521 cgccgtgcga aaacgccacg cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg
     5581 gcggtttacg tttgcctttt ttctttttgc gatcttccgg aatgcggtga atacggtcac
     5641 gctttttcgt cccgctccag tagcgtcgca gcaggaatat caggcctccc gcaatgacca
     5701 ggccgacgat aggggaaaca atcagcgagg agaaaatttt ggtcacttca cgcaggttta
     5761 acgcatccat caccgatgag ccggttaaca gcgcgttggt taaaccgatg ccgataatcg
     5821 caccaatcaa ggtgtgcgaa ctggaggccg gtaaaccgaa gaaccacgtt cccaggttcc
     5881 agataatcgc cgccagcagc atggaaaaga ccatcgccag gccgtgggtt gaccccatat
     5941 tcagcaacaa atcggttggc aacatatgga caatggcata ggcaacgcta agtccgccca
     6001 ataacacgcc aaaaaagtta aaaaatgccg ccatcaccac agcaagttgt ggttgcatgg
     6061 cacgagtata aataacggtt gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt
     6121 cgtagaacaa cacaaatgcc agagcaagca ataacaaaag ccctgtgtat atatcaaggc
     6181 caacaaataa atttagcata tattagatta cgccattttg aatttacgaa cggacgcatt
     6241 atcagtgact ttaacggcat gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg
     6301 ttatttcttg tgtatcagac aataagcatt cattcaaaac ctttagaaat aatcaaaaca
     6361 caggtattcc actggttaat atattcacat atgaaatgaa taaatattgg aatatataaa
     6421 tattgaatat tttgattaat ccccgtactg attattcttc ataatcagta cggggttgct
     6481 acaacatgta ttactttttc accacaatta acggttcaat atcactctct tttttgatga
     6541 ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac
     6601 atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga tagatgtttt
     6661 tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg ctgacgaggt
     6721 cgatattgct gccacagcga ccggcgatcg gtttcactgc gtaacctgtt ttcaccagtt
     6781 catcattaac agtgaaatcg gtatccagca ggtaacgatg gtacgggaac agcgaccaga
     6841 ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca aagaccagca
     6901 cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga ccggtacgga
     6961 ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc
     7021 acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc agttgcccgg
     7081 cagcatccca gcccagttca tccagcccgc gcaagatacg cgtttcaaag cccgcctggt
     7141 gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg tcctgcatga
     7201 tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccagttcg ttaatcagcc
     7261 cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt tcgaggatca
     7321 agcccgcttc ggtatgacat gaggcagagt cggcgttgta ctcgtaaacc ttcagaccac
     7381 gctcatccat gcagaaatcc atacgaccgg tgatcatatg gtgacggcga cgctgccagg
     7441 agagacgcaa acgtggccag aggattttcg ggatgtcgaa cagcgccagc aggttgtcat
     7501 ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg cagctcgttg gtggctttaa
     7561 ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg ataaggatcc tgattgatca
     7621 cctgaccatt ggcctgcaca taggcgtttt gcagcggatc tttttcatcc agccatttac
     7681 cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat tttcagcagc tcgcctgcaa
     7741 tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag cccagaatgg
     7801 tggtgtcatc aaaagtgtct ttcagggtat agcagccgtt ttcgaccacc atctccagct
     7861 cacgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt tccgcaatac
     7921 ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct ttaaattcac
     7981 cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa
     8041 atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa
     8101 tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag agaaaacggc
     8161 gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata tagctacgga
     8221 atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa gagtagattg
     8281 ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc
     8341 ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa ttgttgtgct
     8401 ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata cacctcatcg
     8461 cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga cctgctacac
     8521 tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt atcagcccgc
     8581 gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca atcgcccggt
     8641 ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa cacccattgc aactttattc
     8701 gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctgc tggcgctggg
     8761 cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg atcaattctc
     8821 cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg atcatacgca
     8881 taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt
     8941 tggctacttc ctgccgcagg atttggcaaa gcgtactgaa acgatgaact ggctgttctg
     9001 gttacagggc gcggcaccgt tcctcggcgg tggttttggt cacttttacc attacgcgcc
     9061 gatgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaaacgtc tgctcgacgt
     9121 gctggataag caactggcgc agcataagtt tattgcgggc gatgagtaca ccattgcgga
     9181 tatggcgatt tggccgtggt ttggcaatgt ggtgttaggc ggcgtgtatg atgccgcaga
     9241 gtttcttgat gcgggcagtt ataagcatgt acaacgctgg gcgaaagaag taggcgaacg
     9301 tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac ggaccgctga atgagcagtt
     9361 gcatgagcgc catgacgcca gtgatttcga gacgaatacg gaagataagc gtcaggggta
     9421 agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac gccgcatccg
     9481 gcaatcgtgc ac
//
LOCUS       DAHBHC010000130         9186 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHBHC010000130 DAHBHC010000000
VERSION     DAHBHC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.132"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(182..3628)
                     /gene="mfd"
                     /locus_tag="M2A35_004622"
     CDS             complement(182..3628)
                     /gene="mfd"
                     /locus_tag="M2A35_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCB7200825.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEATNLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESK
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3756..4829)
                     /locus_tag="M2A35_004623"
     CDS             complement(3756..4829)
                     /locus_tag="M2A35_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCB7200826.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFTPPSLPAAVSYSR"
     gene            5091..6290
                     /gene="lolC"
                     /locus_tag="M2A35_004624"
     CDS             5091..6290
                     /gene="lolC"
                     /locus_tag="M2A35_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCB7200827.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6283..6984
                     /gene="lolD"
                     /locus_tag="M2A35_004625"
     CDS             6283..6984
                     /gene="lolD"
                     /locus_tag="M2A35_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCB7200828.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6984..8228
                     /gene="lolE"
                     /locus_tag="M2A35_004626"
     CDS             6984..8228
                     /gene="lolE"
                     /locus_tag="M2A35_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCB7200829.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8257..9168
                     /gene="nagK"
                     /locus_tag="M2A35_004627"
     CDS             8257..9168
                     /gene="nagK"
                     /locus_tag="M2A35_004627"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCB7200830.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
BASE COUNT         2135 a         2355 c         2505 g         2191 t
ORIGIN      
        1 aacgttagcc agcgagaaaa acgcgttttg atcatcacca aaaatccata ataaatataa
       61 ggttattgta ataaacagat aagcctgaat tatggatggt gacagtgtcg gatagtgcag
      121 ggaagtgcaa agaatttgta aatgttgcag atgggggcgc agaaacgccc ccgatttacc
      181 attaagcgat cgcgttctct tccagttcac gcataaactg gcgtacccat tcgatacgcg
      241 ttttccgctc actcaaatcc tgaataaatt tcaggcgcgt cggaccatca aggcggtaat
      301 gctgcggctg tttttgcagc aaaccaatca accaggccgg attaacgtga ttcttctcgg
      361 caaattcgat caccccgcct ttctcattac cttccagctt cctgatcccc agtttctgcg
      421 cttgctggcg cagacgggca acatccagca gggtacgcgc cggatccggc agcaggccga
      481 agcgatcgat aagctcgact ttgatctctt ccagttcgtt ttccgttttg gcgctggcaa
      541 tacgtttgta gaacgacaga cgcgtgttca cgtcagggat gaaatcatct ggcaatagcg
      601 acggcatccg cagctcgact tctgtttgct ggctggtgag atcttccagc gacggctcgc
      661 gtccggcttt cagtgcatcg acggcgtttt ccagcaactc catatacagc gagaaaccga
      721 tggtttccat tgagccgctt tgttcttcgc caagcagttc acccgcgccg cgaatctcca
      781 gatcgtgcgt tgccagcgca aaacctgccc cgagatcttc cagcgaggca atcgcttcaa
      841 gacgtttttg tgcatcggta gtcatcgctt ttggatgcgg tgtcagcaac catgcatatg
      901 cctgatgatg cgaacgtccg acgcgaccgc gtaactggtg cagctgcgcc agaccgaagt
      961 gatccgcacg ttcaatgata atggtgttgg ctgtcgggat gtcgatcccg gtttcgataa
     1021 tggtggtaca aaccagcacg ttgaaacgtt gatgatggaa atcattcatc acccgttcca
     1081 gttcgcgctc gcgcatctgc ccgtgaccga tggcaatccg tgcttcaggc accagttctg
     1141 ccagccgttc ggcagctttc tggatgtttt ccacatcatt gtagagataa taaacctgcc
     1201 ccccgcgcaa aatttcacgc aggatcgcct cccggaccac caggctgtca tactcacgga
     1261 caaaggtttt aactgccaga cgacgggcgg gcggcgtggc gataatcgac aggtcacgca
     1321 ttccgctcat tgccatattc agcgtgcgtg ggatcggcgt tgcagtaagc gtcagaatat
     1381 ccacgttcgc gcgcatcgct ttaatgcgct ctttatgacg caccccgaag cggtgttctt
     1441 catcgacaat cagcaggcct aaatctttaa acttgacgtc actttgcagc agtttgtgcg
     1501 taccgatcag aatatcgatt ttcccttccg ccacttccgc aaggatttgc gtctgctctt
     1561 tggcgctgcg gaaacgggag atcatttcga tacgtaccgg ccagttggcg aaacggtcgc
     1621 ggaagttgtc gtaatgctgc tgcgcgagaa gggtggtagg caccaacacc gccacctgct
     1681 tgtggttatc tactgccagg aaagcggcgc gcatcgccac ttctgtttta ccaaagccaa
     1741 catcgccgca caccagacga tccattgcca gcggctgaca catgtcgcta agtaccgcat
     1801 taatggcctg cgcctgatcc ggcgtggttt caaacgggaa gctgtcgcag aacaactgat
     1861 actgctcacg atcgtgttta aacgcgaagc cctctttggc ggcgcgttgc gcgtagatat
     1921 ccagcaattc cgccgccaca tcacgcactt tttccgccgc tttctgccgc gcgcgtgacc
     1981 acgcatcgcc gccaagttta tgcagcgggg cgttttcttc cgcgccacct gcgtaacggc
     2041 taatcagatg cagtgacgac accggaacat acagtttggc gtcgttggca taggtgagca
     2101 tcaaatactc gccagtaatg ccgcccgctt cgagcgtggt cattccggca tagcgcccga
     2161 caccgtgctc cagatggacc accggctgac caatatgcag ctccgcaagg ttacggatca
     2221 gtgtatcggg gttgatggtg cggcgagaat cctgacgacg gcgggcaaca cgttcaccga
     2281 gcagatcgct ttcgcagatc agcgccagat tacgcacctt atcgacaaaa ccatgttcgg
     2341 cagcgccaat catcagataa cgcccacggt cgctggcttc atcaagacgc ataatgcgtt
     2401 gcggagcaat tttaattcgt gcgagcagtt cacccagcgc ttcacggcga cctttacttt
     2461 ctaccgagaa caccaccgga ccgtcgaaag tctcgaggaa cttacgcagc gcatccagcg
     2521 gcgctttttg ttgtgcctga acggccaggt ctggcagttt ctggaaacct aaattggcat
     2581 tcgcggcttt tgtcggtaaa tgttcagttt ttagctgcac ccggggccag tttttcagct
     2641 ctgagaagag ctcgtccacc cgcagccaga gcgattgtgg tggcaacagc gggcgcatcg
     2701 gatcgacgcc gcgattctca aaacgcgcca gcgtgtcagc ctggaaacgt tcggcactgg
     2761 tttccagatc gccagtattc acgagcaagg tattggcagg gaaataactg aacagcggcg
     2821 gcagtggttc gctgaagaac aatggctgcc agtactcgat cccggcaggt aatgtgcctt
     2881 tactcacttg ctggtaaata tgttccggat cgcgcttcac ttcgaaggta tcgcgccact
     2941 ggctgcggaa cagttcaatt gccgctttat cggtcggaaa ttcgtgcgcg ggcagcagat
     3001 tggtcgcttc tacttcctcc agcgtgcgct ggctgtcgac gtcaaacacc cgcaggctgt
     3061 cgatttcatc atcaaagaaa tcaagacgat aaggcagctc actccccatc gggaagagat
     3121 ccagcaacgc gccgcgcgtg gcgtattcgc cgtgctccat cacctggtca acatggcgat
     3181 aaccggcgct gtccagttgg gttcgtaatg catctcgtga caggcgctga ccttttttca
     3241 tcaccagcgc atgaccgtgg agaaaactgt gtgggcaaac gcgctgcata agcgtattca
     3301 ccggaacaat cagtacgcca cgctgcatcg tcggtagctg gtaaagggtg gaaaggcgcg
     3361 aggagataat gtcctgatga ggcgaaaaac tgtcgtaggg aagagtttcc cagtccgcca
     3421 gattcatcac catctgatcg gtgaactgac tgatttcatc atgcaaacgc agagcatttt
     3481 gcatatctgg tgcaatgagt accaccggac cggcgtgacg ttcggcaatt tccgctacca
     3541 gcgttgcaca ggctgcgccg gttaactcgc ccagcagacg ctgctcaccc gctttgacgg
     3601 gcagcgtata acgatattgt tcaggcataa cggttgtcag attctcgtta ggatatgcct
     3661 caacatatgg gggcatatct ctgataagca atgctgttca ttatccgtaa tcgacatggt
     3721 ttagcaaatt gaatcgcccc ggctggggcg atggcttagc gggagtaact gacggcagcg
     3781 ggcagtgaag gcggggtgaa caacagatcg ctgaatgcac gctgtgaaag ttttcttacc
     3841 agcaagcccg caacagtaca gatgaacaaa ctgacaaagg gataaaccag caggagtgtc
     3901 agttcgactt gcggcgacca gcgtgctgcg ttcatttgcg caagcagagt taagctgaat
     3961 atttcaacca gaatgcgatg ggtggtgtag atagcaatgg tgttggagcc aatcacattc
     4021 agcaggctgg tggagcgcat accgaatcgt tgctcgtatt gataaaacag cttcatgatc
     4081 accacaatcg ataccagcga caacagcagc gagatagtaa acaaccaggc accgaccgcc
     4141 agaacggtaa gcaaagaagc catcagcaga tggcggcgca acggcacctc tttaacacag
     4201 gtcattattg ttgcgccaaa ccatgcgcca aggctgtaat aaggcaaatt gcggatcaca
     4261 ctgttcattc cccaccacgg cgtgggaacg aaattaaccg ccacactcaa cagtacaaac
     4321 aaggcgaata gtggcagcgc cagacggcta aaaattttac ataccacgaa atagacaatt
     4381 aacgcataca gataccacaa gctggtgctg gcggtgatca tcccgtgcag gaactcaccg
     4441 gtggaatcgg cataagcggc attggaggca ttgcttaaat cgcgctcagg tgccagccac
     4501 tggttcagcg cacttaacgc cagccactgc accacgcccc aaagcgccag cacccagaag
     4561 atgttccaga tccgtttatc gagacaattt ccccacggta cgctgtcgat atagcggcga
     4621 atcaaatagc cggaaataaa gaaaaaaacc ggcatacgaa agggggcaag gtaaagattg
     4681 aaatagatcc agcatttgct caggacttcc gataacggat gctgaaaagt ggtcagatgc
     4741 ggataaaagg taatgaccga gtgataaatc accaccagac aaatacataa ccctttgatc
     4801 tggttaatcc atagctcttt ttgtttcatc aggacaacac gctctttttt aatttacatg
     4861 cggttgatcc tgcgggttat gacccgaggt gtaatgggta tctgtctgta ttggatgatt
     4921 tttcagatta agatcaggcg gcaagattga tgataaaaca tggcaattta gccgattgat
     4981 ttacgggggc ttttcagatt agccctgacg atcacttaca gttcagacgt ttacccatct
     5041 tgctttcgct tatatactcg tgtctttgct acagcaacca gacggatttc atgtaccaac
     5101 ctgtcgctct atttattggc ctgcgttaca tgcgtgggcg tgcagcggat cgcttcggtc
     5161 gtttcgtctc ctggctttct accatcggca ttaccctcgg ggtgatggcg ctggtcacag
     5221 tattgtcagt gatgaacggc tttgagcgcg agctgcaaaa caacatcctt ggcctgatgc
     5281 cacaggcaat tctctcttct gagcatggct ctcttaaccc gcagcaactc ccggaaacgg
     5341 cagtcaaact ggacggcgtt aatcgcgtcg cacctattac taccggtgat gtggtactgc
     5401 aaagcgcgcg cagcgtggcg gtcggggtga tgctcggtat cgacccggcg caaaaagatc
     5461 cactaacgcc gtatctggtc aatgtgaaac aaactgacct cgagccgggg aaatataatg
     5521 tcatcctcgg cgaacaactt gcctcacagc taggcgttaa tcgcggtgat caaatccgca
     5581 tgatggtacc atctgccagc cagttcacgc cgatggggcg tattccaagt cagcgcctgt
     5641 tcaatgtgat tggcactttc gccgccaaca gtgaagtcga tggcaatgaa atgctggtga
     5701 atattgagga tgcctcgcgt ctgatgcgtt atccggcagg caatattacc ggctggcgtt
     5761 tgtggctgga tgagccgctg aaagtcgact cattaagtca gcaaaaactg cctgaaggca
     5821 gcaaatggca ggactggcgt gatcgtaaag gcgagttgtt ccaggccgta cgcatggaaa
     5881 aaaatatgat gggtttactg ctgagcctga ttgtcgccgt tgcggcgttt aacattatta
     5941 cctcactagg gctgatggta atggagaagc agggcgaagt agcgatcctg caaacgcaag
     6001 gcttaactcc gcgacaaatc atgatggtct ttatggtgca aggggccagc gccgggatta
     6061 tcggtgcgat cctcggagcg gcgcttggcg ccctgcttgc cagccagtta aataatctga
     6121 tgccgataat cggcgtcctg cttgatggcg cggcgctgcc ggtcgctatc gaacctttac
     6181 aggtcattgt tattgcgctg gtggcgatgg ctatcgcgct gctgtctacg ctttaccctt
     6241 catggcgcgc tgccgccact caacccgctg aggctttacg ttatgaataa gatcctgttg
     6301 caatgcgaca acctgtgcaa acgctatcag gaaggcagtg tgcaaaccga tgtgctgcac
     6361 aatgtcagtt tcagcgtcgg cgaaggtgaa atgatggcga tcgtcggtag ctctggttcc
     6421 ggtaaaagca ccttgctgca cttgttgggc gggctggata cgccaacctc cggcgatgtg
     6481 atctttaacg gtcaaccgat gagcaaactg tcttcggcgg cgaaagcaga gctgcgcaac
     6541 cagaagctgg gctttattta tcagtttcac cacctgctgc cggattttac cgccctggaa
     6601 aacgtggcta tgccgctgct tattggcaag aaaaagcccg ctgaaatcaa cagccgtgca
     6661 cttgagatgt taaaagcggt ggggctggag catcgtgcga atcaccgccc atctgaactt
     6721 tctggcggcg aacgccagcg tgtggcgatt gcccgtgcgc tggtgaataa cccgcgcctg
     6781 gtactggcgg atgaacctac cggtaacctc gatgcgcgta acgccgacag catcttccag
     6841 ttgcttgggg aattgaatcg cttgcagggc accgccttcc tggtggttac tcacgacctg
     6901 caactggcga aacgtatgag ccgccagtta gaaatgcgtg atggtcgtct gacggcggaa
     6961 ctgagcctga tgggggcgga gtaatggcga tgcctttatc gttattaatt ggcctgcgtt
     7021 ttagtcgcgg acggcgacgc ggcggcatgg tgtcgctaat ctctgtcatt tctaccattg
     7081 gcattgccct cggcgtggcg gtattgatcg tcggcttaag cgcgatgaac ggctttgaac
     7141 gcgaactgaa caaccgtatt ctggcggtgg tgccgcatgg tgaaatcgag gcggtggatc
     7201 agccgtggac taactggcag gaagcactgg atcacgtgca aaaagtgccg ggtattgccg
     7261 ccgctgcgcc gtatatcaat ttcaccgggc tggtggaaag tggcgcgaat cttcgcgcaa
     7321 tccaggtgaa gggcgttaac ccgcaacagg aacagcgtct gagcgcatta ccctcgtttg
     7381 tccagggcga tgcgtggcgc aattttaaag cgggcgaaca gcaaattatc atcggcaaag
     7441 gcgtggcgga tgcgctgaaa gtgaagcagg gggattgggt gtcgattatg atccccaact
     7501 cgaatcccga gcataaactg atgcagccaa aacgtgtgcg tttgcacgtt gccggtattt
     7561 tgcagttgag tggtcaactc gatcacagtt ttgccatgat cccgctggcg gatgcccaac
     7621 aatatcttga tatgggttcc agcgtgtcag gtattgccct taaaatgacg gatgttttca
     7681 acgccaataa gctggtacgc gatgcgggtg aagtgaccaa cagctatgtt tatattaaaa
     7741 gttggattgg tacttacggc tatatgtatc gcgatatcca gatgatccgc gccattatgt
     7801 atctggcgat ggtactggtg attggcgtgg cctgtttcaa catcgtctcc accttagtga
     7861 tggcggtgaa agacaagagt ggcgatatcg cagtattaag aacgctgggg gcgaaagatg
     7921 gtttaattcg cgccatcttt gtctggtatg gattgctggc agggctgttc ggcagcctgt
     7981 gtggggtgat tatcggtgtg gtggtttcac tgcaacttac cccgattatt gagtggattg
     8041 aaaagttgat cggtcatcag ttcctctcca gcgatatcta ttttattgat ttcctgccat
     8101 cggaattgca ctggctggac gtcttctacg tactggtcac agcattgttg ctgagtcttt
     8161 tggcaagttg gtatccggcg cggcgcgcca gtaatattga ccctgcgcga gtccttagcg
     8221 gccagtaaag gcagtacatt aaaacaagga gcggcaatgt attacgggtt tgatattggt
     8281 ggaacaaaaa ttgcgcttgg cgtgtttgat agcggtcggc agttgcagtg ggaaaagcgg
     8341 gtgccgacac cgcgtgacag ctatgacgca tttttagatg cagtgtgtga gctggtagct
     8401 gaagctgacc aacgttttgg ctgtaaaggt tctgtcggca tcggtattcc gggaatgccg
     8461 gaaacagaag atggtacgct gtatgccgcc aatgtccccg ctgccagcgg taaaccgctg
     8521 cgtgccgacc tgagcgcacg tcttgatcgc gatgtacgcc ttgataacga tgccaactgt
     8581 tttgcccttt cagaagcctg ggatgatgaa tttactcaat atccactggt gatggggttg
     8641 attctcggca ccggcgttgg cggcgggctg attttcaacg gcaaaccgat taccggtaaa
     8701 agctacatta ccggcgagtt tggccatatg cgtctgccgg ttgatgcgtt aaccatgatg
     8761 gggttggatt tcccgttacg ccgctgcggc tgtggtcagc atggctgcat tgaaaattat
     8821 ctgtctggtc gcggttttgc gtggctgtat caacactatt atcatcaacc gttgcaggct
     8881 cccgaaatta ttgcgcttta tgatcaaggc gatgagcagg caagggcgca cgttgagcgt
     8941 tatctggatt tattagcggt ttgtctggga aatatcctga ccattgttga ccctgacctg
     9001 gtcgtcattg gtggtggctt atcgaatttc ccggcaatca caacgcaact ggcggacagg
     9061 ctgcctcgtc atctcttacc tgtagctcgt gttccgcgca ttgaacgcgc gcgccacggt
     9121 gatgcgggag gaatgcgtgg tgcggccttc ctacatctaa ccgattaaac aacagaggtt
     9181 gctatg
//
LOCUS       DAHBHC010000131         9182 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHBHC010000131 DAHBHC010000000
VERSION     DAHBHC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.133"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..194)
                     /locus_tag="M2A35_004628"
     CDS             complement(<1..194)
                     /locus_tag="M2A35_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200831.1"
                     /translation="MLSREDFYMIKQMSQQGAYIVDIATQIGCSERTVRRDLKYPEPP
                     AKKARHKMVKLKPFMDYIDM"
     gene            complement(376..576)
                     /locus_tag="M2A35_004629"
     CDS             complement(376..576)
                     /locus_tag="M2A35_004629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200832.1"
                     /translation="MLMQHVVGVRYLEYYRATIYAMKHSAMPEIAKMNRPQRYHPDKC
                     SAHAQRDDWLKREIQRVYDDNH"
     gene            981..8744
                     /locus_tag="M2A35_004630"
     CDS             981..8744
                     /locus_tag="M2A35_004630"
                     /inference="COORDINATES: protein
                     motif:HMM:NF023422.1,HMM:NF024320.1,HMM:NF024321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HCB7200833.1"
                     /translation="MRVETVKQEYHKNDNIEQHIKKTRSKRRIKTASMDSVIEIGKRI
                     QAEISSTLQPNNYKIASTKGNWKSALMIRAGLLFSNYQSMSTEKTREYKAVISHGGFS
                     GFHLKHHILNNEKDAAMNDKFKYMENENRIHLKKMRKRRSVDDVIDDKQLNKKTIYDI
                     TSDNIDKKIGWNIEKKNKISPELIDKLKSNIDKYQELSDKNSREGIYLLKTQSNALSQ
                     IYKNLQVNEDNKLLLSNIKSIIKQIQNEYVSHKVSIEKNIHVVWVAGAPPDSITKYAT
                     AYKLAYPDFIFNLWIDIKAMGAYIFNKILREIAFDNAKHDLIASLNKEELNIIKNNVP
                     IGKKLQDKLARSFEYNLLKSQIKIQDSVMNYAYIRGVLNFSDGERIGFLKDILNYDEK
                     KIESFQAEINKNRERTDLIKEKLISILGEDKVVIHDVSTLSDMKIVHRKQHYQQELIL
                     RWNYASATDQLRMYILKEHGGIYTDCDVTPGYTKAVYNILEENSNNFDFLEKEECRRA
                     FNDEILSIASNEESSGYKNKLSNDEKTRLDKIIHEIKKIGTDNLFSPIDTTVIRDSML
                     MSKRYQWWGEDKGWNIRGNNNFLATHKGSKVTEFVISGQDNAYRQLFDIREKLRTESI
                     NVQHHYYAGDNIEEINSGSIEKNTPLDHKEYGIGSLFSKYRMDGIIPRVYSTLHITGP
                     DAIMKSMRDYYNSLGALGQCRLDPSNKHFKGLSQDSFIGNLKRINGIEGEHYDWKNQE
                     SFGINDITPDDVSSWTAKVIDIKSFLYDIVYRDQPNTSFPPPVIEIDVKRLTIGWPSE
                     VKGKLRSLWPNFEDEYNRLLNGNFINLNSLSNIDKKIHQYLILSENKLVKWSGISLAE
                     KLNDYLNKFTIPIGNKVHYLLSEISEQHDEYKKSMFSVLTSDPDAKLVIWRNDKYNKY
                     LVIKELSILQGRKSKIEELMKECSPQQHDNLKIYHRLKRKEQLGMITPEEQLSLIDTI
                     TLISEDQGLKQRIIDIENTLHSFYIEKKLVSNLDKIQNIKSLKGVGSYRDIEYLWNRY
                     VGKEQEILNDIMNNAQSRNLQDKIEIKDVSNELSHSTLINKLVYDGYGFDDFKQVMKY
                     NILYREPGLLLQKAVVAVPSGELVDIINKHTSNNDVETSAILNKLYQHFFGDKTVDFD
                     SSIEEQRIKINFELVLSELDLDNLGKYFLSPLIQNVSPFGVKFSSDNGVLTSDIIASG
                     LRSFVSDERSVVLDKMNDYLSLLYDIKKDIYSYNRINVELIKTMFHKASLSFMLKDDL
                     NLNPLLDKNLHGGDISLTELSQILTGNKNFVECSNVITFGDYPSITNNIIKDIKSSLP
                     SIYSMSDLSGIEQRTLKGIGYIGNTEYILTTPSFRKLHNASIQAKYRTLNWSDFYGRN
                     ARIWQEMVMKLGGGNVRYHPQMLLTPEEGRCIGLSELYLLVKNVNTFQTLQKNIDLAS
                     SLYQDFLLNPSYLTERNRKFLDSVLSQIQYAQNHGNNVLLQSADIDSIRLSDFSINDA
                     VKYLLERNTKNILVVTEYHSMAVLNIDGRYRVVDPNFGYADFSILENALGFIKQSIQI
                     TPEVHELYTGDLSNRRLDILFSKKQDWDHIVSTDLFLLTKFEHESTQEILKKNNAAIS
                     IGGREFNVLNLYKYGVLFNGNRVDESVTKVPTGELEGRSFHIDKNILGEYINNNYLTS
                     DVIDEITVLERLLYGHNNNIKELLKNAEGKNVENLLSRHNDRFLNVIRNTYTELNDKI
                     KKYLSEGFKLNDIIHSDSNAVTLLLSNHETKKHIDVDISEIKMSLREGVNTLSDAIDN
                     MNLDGIMSILGIIQYSRLAYTGAYIGPLDQAGFVSDTKNILEKIVGVTLISIGERKFG
                     TTISSINLESIAAQKISELAVSIGGRTGKMLSRLSSVVKFPVLDTTLNFWSLGESIKA
                     YTSISEDSLDKKLAEIDLVFSSVSTALTLSSFVYPPLGFATFPLLFLQQDVRNFETAL
                     YQDKVRRDAWKEVEKYLDESAKKIIDVDNKNGIINLSSCEIIGDVVIDLSVSPPKISG
                     RKSYNYGKDIGSVPFLSDEEVRKKSKYAISCTDSSDLNINNMFGSVKEQVCSDLSSFT
                     NIVTGFANRVWPKDMPVIQEGNYKTVILGYSSQIKANTEVIRTAWNNFQEVAREGMPL
                     VEKLYRNSKIISGDNLINVVIPKLSTKHFSTEDINLFNELSNYSFTIEGGRGGVVVYS
                     NGVGHFNIKCRHGVKNTLSFRELNERTDAIINIRLDLNKNDKQTVVSYKWLTQPFPHH
                     IMMSLIQENINTVVGSDIGKNVFIGNNDSNHFIIGSASTKLHLGEGDNVITIDKIKHS
                     RVHLDIYPGSAESTQYIQLGCGIDKLLHVNKHKDRLHLYFDEFFDKIITIHNIIKQPG
                     VYIQPVFYLSSEDGLELYINKNGYVIVDKVDVLKYLKYHGYQGIFEPDELPSVNKKIK
                     FDNTSFLYENYIVEVNGDNLIYKVFNPIRNIYASTSKTSIIYGCKSGVYYFWGVRQPT
                     HNLFIYNNNDYPETIDVSSFTSTETEIKITAQCIADTCKLSVYDNNCISTIYIRPTSI
                     ENGELRESSAKIIVDFGKVVTVKDIFSLSASSVNQVVIYNNKWRSELAS"
     gene            9054..9182
                     /locus_tag="M2A35_004631"
                     /pseudo
     CDS             9054..9182
                     /locus_tag="M2A35_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3359 a         1357 c         1711 g         2755 t
ORIGIN      
        1 tgcatgtcta tgtaatccat aaacggtttc agcttaacca ttttgtggcg ggctttcttg
       61 gctggcggtt cagggtattt gaggtcgcgt ctgaccgtcc gttcagaaca accaatctga
      121 gtcgcaatat cgacaatgta cgcgccctgc tggctcattt gctttatcat gtaaaagtcc
      181 tctctgctca gcatgttgat atcctttctg gtgtgagaac cttaaggaaa caacatgttg
      241 ggtggggcgg gcaatccaca tggtgaatta ccgtcttata tcactggcgc tgacatcact
      301 ttatggatac cgtcagtcca ttctccggtg gaaaacattg actggctaaa gtgtagcagt
      361 attaggatca gttatttagt gattgtcatc gtatacgcgc tgtatctctc tcttcaacca
      421 gtcatcgcgc tgtgcatggg cactgcattt atcaggatga tatcgctggg ggcggttcat
      481 tttagcgatt tcaggcatag cagagtgttt catcgcatag atggtcgcac ggtaatactc
      541 caaatacctt acacctacaa catgttgcat caacatagaa atatccttct tacttatccg
      601 aggcaattgc aatatctctg tgaacttaca cactactcta accaggggca gtctacagag
      661 ggttgacagt attcctatgc taattgttaa gattcaaatt gaacaatctc tatctgtcat
      721 tttgagcatt tttaaatggc atgcggtagc tttacctcac tatcacgaac atttcgagaa
      781 aaaatcaaca aagccgttag aacggtgatt ttcaaatgcg tgatggcatg gttgagataa
      841 ttatcgatga gctgataaaa tataatatta ttggggacga aagcactacg ctgttgctga
      901 atactttctg tttagttaca acaaatgttg ggaactatat atcttcactg taaataaaag
      961 taattgaagg agagacgtaa atgagagtag aaaccgtaaa acaagaatat cataaaaacg
     1021 ataatataga acaacacata aaaaaaacaa gaagcaagag gagaataaaa acggcttcaa
     1081 tggatagtgt tatagaaatt ggaaagcgta ttcaagctga gatcagctca acgctacagc
     1141 cgaataatta taaaattgca tcaacaaaag gaaattggaa atcagccctc atgatcagag
     1201 caggacttct cttttccaat tatcaaagta tgtcaacaga gaaaaccaga gaatataaag
     1261 cggttatatc acatggaggt tttagtggtt ttcacttaaa acaccacata ttaaataatg
     1321 aaaaagatgc agcgatgaat gacaagttca aatatatgga aaatgaaaac cgtatacatc
     1381 taaaaaaaat gaggaaaaga agaagtgttg atgatgttat tgatgacaaa caattaaaca
     1441 aaaaaacaat atatgatata accagtgata atatcgacaa aaaaataggc tggaacatag
     1501 agaaaaaaaa caaaatatct ccagagttaa ttgataaatt aaaatcaaac atagataaat
     1561 atcaagaact aagtgataag aatagccgag aaggaattta tcttttaaag actcaatcaa
     1621 atgcattaag tcaaatatat aaaaacttac aggtaaatga agataacaaa cttttgctga
     1681 gcaatataaa aagcattata aaacaaattc aaaacgaata tgtttcacat aaagttagca
     1741 tagaaaaaaa tatacatgtt gtttgggttg caggggctcc accagatagc ataactaaat
     1801 atgccaccgc ttataaattg gcatatcctg attttatatt taatttgtgg attgatatca
     1861 aagctatggg agcatatatt tttaataaaa ttcttcgtga gattgcattt gataatgcaa
     1921 aacatgactt gattgcctcg ttaaataaag aagagttaaa tataataaaa aacaatgtgc
     1981 ctattggtaa aaaacttcaa gataaattgg ctagatcgtt tgaatataat ttgttgaagt
     2041 cacaaataaa aatccaggat tcagtcatga attatgctta tatcaggggg gtgttaaatt
     2101 ttagtgatgg tgagcgtatt ggttttttaa aagacatatt aaattatgat gaaaagaaaa
     2161 tagaatcatt ccaagctgaa ataaataaaa atagagaaag aactgatcta ataaaagaaa
     2221 aattaataag tattcttggt gaggataaag tagtaataca tgatgtaagt actctttctg
     2281 atatgaagat agtacatcgc aaacagcatt accaacaaga gttgattctt agatggaact
     2341 atgcatcagc aactgatcaa ttacgtatgt atatattaaa agagcacggt gggatttata
     2401 cagattgtga tgtaactcca ggatatacaa aggcagttta taatatcctt gaagaaaata
     2461 gtaataactt cgatttttta gaaaaggaag agtgtaggcg agcgttcaat gatgaaattc
     2521 tttctatagc cagcaatgaa gaaagttctg gttataaaaa caagcttagc aatgatgaga
     2581 aaactaggct tgataaaata attcatgaga taaaaaaaat aggtaccgat aacttatttt
     2641 ctccaattga cacaacggtc attcgtgact ccatgcttat gtctaaaagg tatcaatggt
     2701 ggggagagga taaaggatgg aatatccgag gtaataataa tttccttgca actcataaag
     2761 gtagcaaagt tacagagttt gtaatatcag ggcaggataa tgcatacagg caactatttg
     2821 acataagaga aaagttaaga actgaaagca taaatgtaca acatcattat tatgctggtg
     2881 ataacataga agaaatcaat agtggcagca ttgaaaagaa cactcctctc gaccataaag
     2941 aatacggcat tggttcttta ttctctaaat acagaatgga tggaattata ccaagagtat
     3001 acagcacgtt acatatcact ggacctgatg ccataatgaa gtcgatgaga gattattata
     3061 attctctggg agcgttgggg caatgccgcc tagatccctc taataaacac tttaagggat
     3121 tatcacaaga ctcctttatc ggtaatttaa aaagaataaa tggtattgag ggagagcatt
     3181 atgactggaa aaatcaagaa agttttggga ttaacgatat tactccagat gatgttagct
     3241 cgtggactgc taaagttata gatataaaat cttttttata tgacattgta tacagagatc
     3301 aacctaatac ctcattccct cccccagtta tagagataga tgtaaaaagg ttgaccattg
     3361 gatggccaag tgaggtgaag ggtaaattac gttcattgtg gccaaacttt gaagatgaat
     3421 acaacaggtt actgaatggc aattttatta acttaaactc tttatccaat atagataaaa
     3481 aaatacacca atatcttata ttatctgaaa ataaactagt taagtggtca ggaatatcat
     3541 tggcagaaaa attaaatgac tacttaaata agttcactat accgataggg aataaagttc
     3601 actatttact aagtgagata agtgagcagc atgatgaata taaaaagtct atgttttctg
     3661 ttttgactag cgatcctgat gctaagttgg tgatatggag aaatgataaa tacaataaat
     3721 atttagttat aaaggagcta tctattttac agggaaggaa gagcaaaatt gaagaactaa
     3781 tgaaagagtg ctctcctcaa caacatgata atttgaaaat ataccataga ttaaaaagaa
     3841 aagaacaact aggcatgata acaccagagg agcagttaag tctaattgat acaataacat
     3901 taatctcaga agaccaggga cttaaacaga gaataataga tatagagaac acactccact
     3961 ctttttacat agaaaaaaaa ttagtttcta atcttgacaa aattcagaat attaagagct
     4021 taaaaggtgt tggtagttat agagacattg aatatttatg gaatagatat gtcggaaaag
     4081 aacaggagat tctcaatgat attatgaata atgctcaatc ccgaaaccta caagacaaaa
     4141 tcgaaataaa agatgtatca aatgaactgt cacactctac attaataaat aaattagttt
     4201 atgatggata tggctttgat gattttaaac aagtaatgaa gtataatata ttatatagag
     4261 agccaggtct tcttcttcaa aaagcagtcg tagcagttcc atctggagag ttagttgata
     4321 tcattaataa gcatacatca aataatgatg tggaaacttc tgctatatta aataaattat
     4381 atcaacattt ttttggtgat aaaactgttg attttgattc ttccattgaa gaacagagga
     4441 ttaaaattaa ttttgagctg gttttaagtg agctggatct tgataactta ggaaaatact
     4501 ttctgtcacc attaatacaa aatgtttcac cttttggtgt taagttttct agtgataatg
     4561 gtgtgttaac atcagatata attgcttctg gcttaagaag ttttgtatca gacgaaagga
     4621 gtgttgtatt agataaaatg aatgattatt tatcattgct ttatgatata aaaaaagata
     4681 tttattccta taataggatt aatgttgagt taataaaaac gatgttccat aaagcatcgt
     4741 taagttttat gctgaaggat gatttaaatc ttaatccgct attagataag aatcttcatg
     4801 ggggggatat ctctttgaca gagctaagcc agatattaac aggcaataaa aattttgtag
     4861 aatgttccaa tgttataact tttggagatt acccatcaat aactaacaat atcataaaag
     4921 atataaaatc gagcttacca tcaatatata gcatgtccga cttatctgga attgaacaaa
     4981 ggacattaaa agggattggc tacatcggta atacggaata tattctaaca acaccttcct
     5041 tcaggaaatt acataatgca tctatccaag caaagtaccg cacattaaac tggagcgact
     5101 tttatggtag aaatgcgcga atatggcaag aaatggtaat gaaattggga gggggtaatg
     5161 tgagatatca tcctcagatg ctattaactc cagaggaagg gagatgcatc ggtttatctg
     5221 agttgtatct cttggttaag aatgtaaata cattccaaac attacagaaa aatatagatt
     5281 tagccagttc attatatcaa gattttctgt tgaacccctc ttatctgact gaacgaaata
     5341 ggaagttttt agactctgtt ctttctcaga ttcagtatgc tcaaaatcat ggaaataatg
     5401 tattattaca gtctgcagat attgatagta taagattgtc tgacttcagt attaatgatg
     5461 ctgttaaata tttattggaa agaaacacga agaatatatt ggtagtgaca gaataccata
     5521 gtatggctgt tttaaatatt gatggcagat atcgggttgt tgatcctaac tttggatacg
     5581 ctgacttctc aatattagaa aatgcattag gatttattaa gcagtcaatt cagattacac
     5641 cagaagtaca tgaattatat acaggagatt tatcaaacag gagattagac attctcttct
     5701 caaaaaaaca agattgggat catatagtat ccacagactt atttctcctt acgaaatttg
     5761 aacatgaatc aacacaagaa atactcaaga aaaataatgc ggcgatatca ataggtggta
     5821 gagagtttaa tgtattaaat ttgtataagt atggagtgtt gttcaatggt aatagagttg
     5881 atgagagtgt tacaaaagtt cccactggag aattagaggg acgtagtttt catattgata
     5941 agaatatatt aggtgagtac attaataaca attatttgac aagcgatgtt attgatgaga
     6001 tcacggtact tgaaagatta ttatatggac acaacaacaa tataaaagaa ttattaaaaa
     6061 atgcagaagg aaagaacgtg gaaaatcttc tttccagaca taatgaccgc tttttgaatg
     6121 tcattcgcaa tacgtataca gaattaaatg ataaaataaa aaaatattta tcagaaggtt
     6181 tcaagttaaa tgatataata catagtgaca gcaatgcagt tacattgcta ttaagtaacc
     6241 atgagaccaa aaaacatata gatgttgaca tatcagaaat taaaatgtcg ttaagagaag
     6301 gggtgaatac attatctgat gcaattgata atatgaattt agatggaata atgtccattt
     6361 taggcattat tcaatactca aggcttgcat atactggggc gtatattggt ccacttgatc
     6421 aagctggttt tgtatcagat acgaaaaata tcttggaaaa aattgtgggt gttacattaa
     6481 tttctatagg agagcgcaaa tttggaacta caatcagctc aattaactta gaaagtattg
     6541 ccgctcaaaa aattagtgag ttagctgtaa gcattggagg aaggacagga aaaatgctat
     6601 ctcgattatc gagtgtagtt aagtttcctg ttttggatac aacactcaat ttttggtcct
     6661 tgggtgagtc aattaaagct tatacctcta ttagtgagga tagtttagat aaaaaacttg
     6721 cagagattga tcttgtcttt tcttctgttt ctacagcgct gacattatca agtttcgtat
     6781 atcctcctct gggatttgca acatttcctc tactgttttt acagcaggat gttcgtaatt
     6841 ttgagactgc cttatatcag gataaagtca ggagagatgc ctggaaggaa gttgaaaaat
     6901 atctagatga gtccgcaaaa aaaataattg atgttgataa taaaaatggt ataataaatc
     6961 tttcatcttg tgaaattatc ggtgatgtag ttattgattt gtccgtatct ccccctaaaa
     7021 tttcagggag aaaatcttat aactatggaa aagacattgg ttctgtaccg ttcctttcag
     7081 atgaagaagt tagaaaaaaa agcaaatatg ctatttcatg cactgattca agtgatttaa
     7141 atatcaataa tatgtttggt tctgtaaagg aacaagtctg ctccgacctg tcatcattta
     7201 ccaatattgt gacaggtttt gccaatagag tttggccaaa ggatatgcca gttattcaag
     7261 agggaaacta taaaacagtt atacttggtt acagttctca gattaaagca aatacagaag
     7321 ttattcgtac tgcatggaat aattttcagg aagtggcaag agagggaatg cctcttgtag
     7381 aaaaattata tagaaactca aaaataatat cgggagacaa tctcattaat gttgtcatcc
     7441 ctaaattatc gactaaacat ttctctacag aagatattaa tctattcaat gagttatcta
     7501 attattcgtt tactatagaa ggaggacgag gtggtgttgt tgtctattcg aatggagttg
     7561 gacattttaa tataaaatgt cggcatggtg taaaaaacac actctcattt cgggagttaa
     7621 atgagcgaac tgatgcaata attaatataa ggttggattt gaataaaaat gataaacaaa
     7681 cagttgtttc ttacaaatgg ttaacacaac cttttcctca tcatatcatg atgagtctaa
     7741 ttcaagaaaa tataaatact gtagttggct ctgatatcgg taaaaatgtt ttcataggga
     7801 ataatgactc taatcatttt atcattggaa gtgcgtccac taaactccat cttggagaag
     7861 gggataatgt tattacaata gataaaataa aacatagcag agttcatctt gatatttatc
     7921 ccggcagtgc agaatcaacc caatatatac agctcggttg tggtatagat aaactactac
     7981 atgtaaataa gcacaaagat agattacatt tatactttga tgagtttttt gataaaataa
     8041 taacaataca caatattatt aagcaaccag gggtttatat tcagcctgta ttttatcttt
     8101 cttctgaaga tggtttggaa ttatatataa ataaaaatgg ttatgttatt gtggataagg
     8161 ttgatgtctt gaaatattta aaatatcacg gttaccaagg aatatttgag ccagatgaat
     8221 taccatcagt taataaaaaa ataaaatttg acaacacctc tttcttatat gaaaactata
     8281 tagtggaagt aaatggtgat aatcttatat acaaagtatt caaccctata agaaacatat
     8341 atgccagcac aagcaaaaca tccataattt atggttgtaa aagtggagtg tattactttt
     8401 ggggggttcg acagccaact cataacctct tcatatataa taataatgat tacccagaaa
     8461 caattgatgt ttcaagtttt acatccacag aaacagaaat taaaatcaca gcccaatgta
     8521 ttgctgatac atgtaaactt agtgtatatg ataataattg tatttctaca atatatataa
     8581 gacctacatc tattgaaaat ggagagctaa gagagtcttc tgctaagatt atagtcgatt
     8641 tcggtaaggt agtgacagtt aaggatattt tttctttatc ggcttcctca gtaaatcaag
     8701 ttgttatata taataacaaa tggagaagcg agttggcttc ctgaatatcc agacagccaa
     8761 tattgatcgt cttaatactg tttttagact agttatacgg cggtgcctaa ataactgctc
     8821 gaaatagtag atcactcagg aggaactcag tccggtttat gtgacctgag caatcgctga
     8881 atatcccaaa tcaccagccg gactgagcaa tgccgatcat agcaccaatc cccagagtcc
     8941 aacgacacct gatgcagaaa actatccata aaacaaagga caaaaactac gcccggcggc
     9001 tcacctctag actggcctcc tgaatctcca gacaaccagt atcacttaaa taagtgatag
     9061 tcttaatact agttttcaga ccagtcactg gagaacggat gattgatgtc ttgggaccgg
     9121 agaaacgcag gcggcgtacc acacaggaaa gatcgctatt gttcagcaga gctttgaacc
     9181 gg
//
LOCUS       DAHBHC010000132         8866 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHBHC010000132 DAHBHC010000000
VERSION     DAHBHC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.134"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..227
                     /locus_tag="M2A35_004632"
     CDS             <1..227
                     /locus_tag="M2A35_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7200834.1"
                     /translation="YIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVI
                     HPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            267..1115
                     /locus_tag="M2A35_004633"
     CDS             267..1115
                     /locus_tag="M2A35_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCB7200835.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NSYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEM
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            1232..1705
                     /locus_tag="M2A35_004634"
     CDS             1232..1705
                     /locus_tag="M2A35_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200836.1"
                     /translation="MRDKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     ASRLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
     gene            complement(2182..2448)
                     /locus_tag="M2A35_004635"
     CDS             complement(2182..2448)
                     /locus_tag="M2A35_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200837.1"
                     /translation="MLTGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(2448..2942)
                     /locus_tag="M2A35_004636"
     CDS             complement(2448..2942)
                     /locus_tag="M2A35_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200838.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(2998..3600)
                     /locus_tag="M2A35_004637"
     CDS             complement(2998..3600)
                     /locus_tag="M2A35_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCB7200839.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            3954..5300
                     /locus_tag="M2A35_004638"
     CDS             3954..5300
                     /locus_tag="M2A35_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200840.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            5579..7459
                     /locus_tag="M2A35_004639"
     CDS             5579..7459
                     /locus_tag="M2A35_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HCB7200841.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(7477..7824)
                     /locus_tag="M2A35_004640"
     CDS             complement(7477..7824)
                     /locus_tag="M2A35_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HCB7200842.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
     gene            7943..8291
                     /locus_tag="M2A35_004641"
                     /pseudo
     CDS             7943..8291
                     /locus_tag="M2A35_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8311..8862)
                     /locus_tag="M2A35_004642"
     CDS             complement(8311..8862)
                     /locus_tag="M2A35_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200843.1"
                     /translation="MLIVLAALDEKRPGPVLTRDIERVLERSGEAPVYGPNLRASCRR
                     LEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQRAAEVVVLPLVPAA
                     GLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVA
                     QWLQACHDAGMEVRVQINESAAP"
BASE COUNT         2365 a         1986 c         2176 g         2339 t
ORIGIN      
        1 agtacattgc ggagcagaac ctgatggccg ccattgaact ggacgaacgg attggttatt
       61 cagccagcag cctggccggg cagccgtata aaggccgcaa cggtcgtgtg gaaggtaccc
      121 gggaactggt tattcacccg cattttgttc tggtttatga ggtcgacagc cagtggggaa
      181 aagtgtatat cctgcgcgtg ttgcatactg cacagaagtg gccatagagg ggctgtccgg
      241 atataaggaa aggagatgca ataaccatga ataatgatga gctggccacc agacgtgcgc
      301 aggcgattgc cgaagacagg tgtttctcga aaggacgttt gcgcgatgaa ttcagaatga
      361 aaccagcccc cggtgctgag ccggttaagt ggtataaaaa tagttatggt ggcaggttcg
      421 ctgtataccg aattgcagac tgtgttccga tgcgtgagaa acgccctctg acgagtaaac
      481 aacagctggc tggccagcgg ctttccgttt tatcccgact aaacagcacc agtggccgga
      541 tggcacggca ggcatatgac tggctgagtc tggcaccact ctttctggat actgaaacga
      601 caggactgga taataccgcg gaagccctgg aaatcggcct gaccgatgcg gccggtcagg
      661 tggtatttga aaccaggctg aagcccacgg tcgccatcgg ggcgcaggcc gccgccgtgc
      721 atggtatcag tgagcaggct ctctgcggcg caccatcatg gacagatgtg gcgcggcaac
      781 tgcgtcatgc aatcggggac cgaccagtaa ttatctttaa tgcccggttc gacatccgca
      841 ttctgaaaca gactgctgcc gcacatagcg atccggctga ctggctggaa gaactgacgg
      901 tatattgtgc gatggagatg gctgcaggat attatggggc caccaaccgc tatggcacga
      961 tttcactggc ctgtgctgcc agtcaggccg gactgacctg ggaagggcag gcacactcag
     1021 cgatcgccga cgcacggatg acggcagggg tggtaaacgc tattgctgca tatcatctgg
     1081 aactgctgca ggaacaggca cggctgaaaa tctgaccgcc tggcctgtat accgcaatca
     1141 tttacgttat ccagacgtaa atccgccgct tgttgctgtc agcatgttaa tgctaatgta
     1201 tgtgtacatt agcattaatg agggatgata tgtgagagac aaaaccaggg aggccatgcg
     1261 gcttttttta ggcggcaggt gctacacggc tgaaaaactt gaaaaggatt atctggctga
     1321 agtggctaat tacagtaacg accgctggga agcaccgcaa cgtgcctcac gcctggctgc
     1381 cagcgtaaag cgttacaaga catcagaaat gctgcgcttt atttttgcta ccattgccta
     1441 cgatcccgat ccagatttaa ccccgctaac tgtgagacgt ttgtgcaagg cattgttcgg
     1501 cagaaccgga agccagtggc tggttgttga agtttttggg gagaaggggc gtcagcaccg
     1561 cagcgcggac agcaatccgg agatggtgga aaaaatggct gccagatatc gtcatgccgc
     1621 cgaactacac tggtccgcaa cgctggcaga aattgaacgg gtaaagcggc tttatcagac
     1681 gaaaattaag aaatccaaaa aataagtggg ttgactgcca ttgttgttct tctccttcat
     1741 cattaatgtt aatgtatgca tacattagca ttaatgatgg tgcgttaaag tatcactggt
     1801 ataatctggt aaagaacata ttgagaatga gaacttatca tgtcgtcacg caactctgta
     1861 catgatttta taatgctgct cccggcgctg ctgttggttg ggtggttagt tttggtgata
     1921 attctcatgt ttagggatga taaatgtgac aaaacttacc gtgaaggttc gtttcccgaa
     1981 tttatttgtc tccattctga tcgctcgtca cttcgagaaa ccgattctga gagactcatt
     2041 gtcaaatata cagaaataaa atcacaaaaa taggtaaaac caataatact atagctaaaa
     2101 aatagttact tttctgtatc accctgtctc tctctgcttt ttcatggcta ctgtcatatt
     2161 tatgtctgtt gcgcggtttt gttacttttt catgaccgaa cccgaaattt tgcgcgtcaa
     2221 taagagacgt tgtgtaaact cagtcctttt atctggcagg ttaaccccta tcaatcttag
     2281 gatgaaacca cgccatcctc tgtttgcctg gttattaatt tcataaagac gattgttttc
     2341 ctctcgtagt tttgtccact ctaaacaaag tgttatatca tccagttctg tgatgtgttt
     2401 cacttttcca ttgacagtta cagtgtttat tccatttcct gtgagcatca tcttatgcct
     2461 tttttatctt catttgggac cgtgcttttc ttcggagtat cactattcgt taactgctgt
     2521 ttatcaataa aattcctgat gttatcaagt gcttcctttg tttttagaat gcgcttgata
     2581 tccatttcat cccgcccggt cattcttttt ccggacaaaa gacgctcaag aagctcctgt
     2641 ttcgcatcag gagaatcttt ggcgagatcc atcaggttaa gcaaatcttc acgatcgaga
     2701 ggaaagaagt attttacaaa atacttgaaa agcgaaagaa agtagtatgc tgtccatgca
     2761 atacagaaac cgattctgag aaactcattg tcaactatac agaaatacag gtatacaatc
     2821 aggagaatag gtaaaatcca taatactata gctaaaaaaa tatatacggg gatcaggcgt
     2881 ctgtatgccc gttcatgttt attcaggatg aataaaatct tctccgcttc tgaatcacac
     2941 ataacattct cttcccattt tttctgatac gtaatgtcca cctgttatca ggagtgttta
     3001 ttgcccagaa atattctcct gctgtgcctg cgtcctgagc cacttttgtt ctaatgtcgc
     3061 cagtctgcga ccggcactct gtgcttcaga gtctgagacc tgccctttgg ggtgaccatc
     3121 caggccataa cgtgggacgc cggcgacagc tgtccgctgc agatatacgc ggcgggatgt
     3181 gatgcaggac actgcacaga gccattcgtc catggtcagt tctgtctccg ggtgttctgc
     3241 cagaaatgcc gaaatatcat tgcttatgcc aattttcagg ggaataaatc caccaccttc
     3301 agcacgaaga tgaggaaaca gggcctttat tttttccacg cactcgtgag tatactcacg
     3361 ggtccagtgg gagggacgtg gaaaacggtg tcttttcttt ttcttcttcc tggctggcgc
     3421 ttcggggata tgtgtttcga cggcagatga atttattccg gcgggggctg attctgccgt
     3481 ctgttcagta aatacaggtg cgataatatc tgttttttcg gataaggacg gtggcgaaaa
     3541 agtacgacgt tttttcacca caatgacggg agttttcctt tcttcctgat tttttcccat
     3601 aaatacctcg gctggctggt tttactcttt aataatatca caatccataa attagtaaat
     3661 ttcccatcag gtgatttttc atggctaata agtccattct ctgaatattt atactcgagg
     3721 tagttacagt tatggcgtga cagtcgaatt gatattctac tgaaccctga cagaaacatg
     3781 gttgatatat tctctcactt taatgttgtc atgatataaa taaaaacaat tcatttgaaa
     3841 ggatatcaga agaagatatc ggggcgcaca gttcatctct gcctttgttt catggggggt
     3901 tctttgtata ttttacttac tgtttaagca tgtacataag gagtgctttc attatgccaa
     3961 tgatacttgt aaagaagaat ccccggggca aagtgattcg ggagcttagc agtgaggaag
     4021 agactgcagt aaagacggtc tgtggactga aaaggccagc aaccatggca caacataacc
     4081 tggccaatga tcttctccgg gaaatgcgtg agtatgatgc atggttacag tgtgactgta
     4141 ttccgggaga ttcgccggcc atgaattttg ctgctcttaa aaacaatacg ggtactcttt
     4201 atctgtccag ttttaatcat gagcatgcac ctgaatgccc catgtatcgt cagttatccg
     4261 gaaatgaaga ggaaacatcc ttcggagcaa gccggcatcc cgttagcacc cgtataaatt
     4321 acagaaactt tttaccacct gatgacagta attctgttat cagactgcag gcccgctctg
     4381 cttaccataa tggtgagcgc tcctccgttc gcaagaaacg tccgcgcctt ggccgtttac
     4441 tcctgtcgct tattgaagat gcagggctga ataaactgga ttccctggct aatccccgta
     4501 tccggactaa ttatcgtgag tgtcttgatg ccatacggca ggttacacta cagcaggagt
     4561 atattcgggg aagagcctta tctgaaatta tccatttccg gccaggaatg tctgaaaggt
     4621 ctcaggagcg gttaatggaa acgctggaga attcagaacg acactggcct gccagaagaa
     4681 agcatatgtt ctttcagatt tttatggcgc agcacatctg tcgtgatgct gtggaaattc
     4741 actgggcgaa cggtaatatt caggtcatca gacctgtgcg ggggatcagt attaatggtg
     4801 aagcgcaggg cggaatacgt cccccttact gggttattct tgctttttgc cggagtgccg
     4861 atggcagaat catctgcagt gaagggtatg ctcatgctct ttatcaactg acatgtccgg
     4921 tgcctgtgga cagcaaactg gaacgaaaca cgctcactgc tcttctgaat gtggccagct
     4981 ggcttaaaag aaagccaggt acgccggaat taagtctgga aaggcccctg tttgatacag
     5041 aagtttatgt taatggtgaa aagaaatatg tactgccgga tttcattgtc acagcaaggg
     5101 ctcctgacgg aaagacggcc agagtggtca tcgaaacgat gggatatgaa gacagtgatt
     5161 actgcgcgag aaaatccagg cagcataccg gcatgaagca gattggtgtt ctgcataccg
     5221 atccaccgaa atggctggat aacgatcatc ccccttttaa gaaacatatg tacggtgttt
     5281 ttatgcatct caggtactga gatattttgt ggctcagttc tgtaactttt cccgtaacat
     5341 tgtctgttgt tacgggaaag tccggttttt gtattgcacc agagaatacc cagactgtga
     5401 tgctgccaca gtgtcagcag gctttctgaa cggtataaca ccacttcatg ttaatgataa
     5461 ttactatcat taaatcttga catgccattt tctccttaat aaattagtac tgtatatgta
     5521 tccatatacg taagcagtta attcatttgt tttcctcaga ggatgaagga gataccgaat
     5581 gtctgaccct gtacgtatta caaatcccgg tgcagaatcg ctgggatatg attcagatgg
     5641 ccatgaaatt atggccgttg atatttatgt aaaccctcca cgtgtcgatg tctttcatgg
     5701 taccccgcct gcatggagtt ccttcgggaa caaaaccatc tggggtggaa acgagtgggt
     5761 cgatgattcc ccaacccgaa gtgatatcga aaaaagggac aaggaaatca cagcgtacaa
     5821 aaacacgctc agcgcgcagc agaaagagaa tgagaataag cgtactgaag ctggaaaacg
     5881 cctttctgcg gcaattgctg caagggaaaa agatgaaaac acactgaaaa cactccgtgc
     5941 cggaaacgca gatgccgctg atattacacg acaggagttc agactcctgc aggcagagct
     6001 gagagaatac ggattccgta ctgaaatcgc cggatatgat gccctccggc tgcatacaga
     6061 gagccggatg ctgtttgctg atgctgattc tcttcgtata tctccccgcg aggccaggtc
     6121 gttaatcgaa caggctgaaa aacggcagaa ggatgcgcag aacgcagaca agaaggccgc
     6181 tgatatgctt gctgaatacg agcgcagaaa aggtattctg gacacgcggt tgtcagagct
     6241 ggaaaaaaat ggcggggcag cccttgccgt tcttgatgca caacaggccc gtctgctcgg
     6301 gcagcagaca cggaatgaca gggccatttc agaggcccgg aataaactca gttcggtgac
     6361 ggaatcgctt aagacggccc gtaatgcatt aaccagagct gaacaacagc tgacgcaaca
     6421 gaaaaacacg cctgacggca aaacgatagt ttcccctgaa aaattcccgg ggcgttcatc
     6481 aacaaatcat tctattgttg tgagtggtga tccgaggttt gccggtacga taaaaatcac
     6541 aaccagcgcg gtcatcgata accgtgcaaa cctgaattat cttctgaccc attccggtct
     6601 ggactataaa cgcaatattc tgaatgaccg gaatccggtg gtgacagagg atgtggaagg
     6661 tgacaagaaa atttataatg ctgaagttgc tgaatgggat aagttacggc aacgattgct
     6721 tgatgccaga aataaaatca cctctgctga atctgcgata aattcggcga gaaataacgt
     6781 cagtgccaga acaaatgaac aaaagcatgc aaatgacgct cttaatgccc tgttgaagga
     6841 aaaagagaat atccgtagcc agcttgctga catcaatcag aaaatagctg aagagaaaag
     6901 aaaaagggat gaaataaata tggtaaagga tgccataaaa ctcacctctg atttctacag
     6961 aacgatatat gatgagttcg gtaaacaagc atccgaactt gctaaggagc tggcttctgt
     7021 atctcaaggg aaacagatta agagtgtgga tgatgcactg aacgcttttg ataaattccg
     7081 taataatctg aacaagaaat ataacataca agatcgcatg gccatttcta aagccctgga
     7141 agctattaat caggtccata tggcggagaa ttttaagctg ttcagtaagg catttggttt
     7201 taccggaaaa gttattgatc gttatgatgt tgctgtggag ttacaaaagg ctgtaaaaac
     7261 ggacaactgg cgtccatttt ttgtaaaact tgaatcactg gcagcaggaa gagctgcttc
     7321 agcagttaca gcatgggcgt tttccgtcat gctgggaacc cctgtaggta ttctgggttt
     7381 tgcaattatt atggcggctg tgagtgcgct tgttaatgat aagtttattg agcaggtcaa
     7441 taaacttatt ggtatctgac acataatgat gccctttcat tttcctgaaa gggctttgca
     7501 gagtataaaa attaagtaaa tggcaaccac aggaactgat ataacgaaac agaataattc
     7561 aaatattgca gttaagcttc cgccaactgg atttgtaaag aagtcttttt gccagaattc
     7621 tttccctgta aaccttatta ccattttctg tattatcctc attgaaaaag gaaaagtaat
     7681 ggcactgata atgcatccga tgaacgcata aaacattctt gcattttcct ggttatcttt
     7741 ccatcccaga taaaaaactg taagaataat gaggattggt atcaggcttt tcagtaaata
     7801 ctttacagat atatccagtt tcatctggtt tctcctttat tgttatcgtg taccggtgcg
     7861 tgataacata atcacagcat gataataata atcaataaca ataagctgtg tcacgtttac
     7921 atcatggaac ggaggtgctc ctgtgacctt tgagcagaaa aaagctcggg ccattgccct
     7981 gatggacagc aaaaagatgt ggcggagcaa ttatgcccct cctcttctgc gtattctctg
     8041 gcggctgggt atccggcttc cgccattacc ctttatgccg ttctggcagg tcacggtgct
     8101 gacggggggc ttatggggca tttcctgggg atgtgcaatg tggtttatct actgggggcc
     8161 gtcaggaatg gtggcaggtg aggccattat tataagtatc acgggggggg gttttgtccg
     8221 gactgtgcat ggcctctttc cactggtggc gcaggaaagt taaccggctg ccaccatggg
     8281 atgatgtgta aaccgtgcac aggcgtgttc ttacggagcg gcactttcat ttatctgtac
     8341 gcgaacctcc attcctgcat cgtgacaggc ctgcagccac tgcgccactt ccagcggatc
     8401 gccctcccgg cgtaccaccc tgccttcttt attccataac tgcagacagg tgctgccgtc
     8461 tagacgcacc acaaaatccc cacggcaggc ctgatacgtt atgccgttca gttgcaccgc
     8521 cagatcagtg gcagaagtgc catctgcggg cagacctgct gccgggacca gcggcagtac
     8581 gacgacttct gccgcacgct gttctgcccg gaggcggtcc tgttctgcca gcagcagagg
     8641 ctgtgccacg gcccggccgg cgtccgtcag ctctactgcc agctgcaggt tcggagcgcg
     8701 cagggtacgc agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc
     8761 gtagaccggg gcttccccgc ttcgctccag cacccgttct atgtcccgcg tcagcacggg
     8821 tcctggccgt ttttcatcaa gggcagccag cacaatcagc actctc
//
LOCUS       DAHBHC010000133         8817 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHBHC010000133 DAHBHC010000000
VERSION     DAHBHC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.135"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1498)
                     /gene="dcp"
                     /locus_tag="M2A35_004643"
     CDS             complement(<1..1498)
                     /gene="dcp"
                     /locus_tag="M2A35_004643"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCB7200844.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVE"
     gene            1635..2381
                     /gene="ydfG"
                     /locus_tag="M2A35_004644"
     CDS             1635..2381
                     /gene="ydfG"
                     /locus_tag="M2A35_004644"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCB7200845.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            2470..3156
                     /gene="ydfH"
                     /locus_tag="M2A35_004645"
     CDS             2470..3156
                     /gene="ydfH"
                     /locus_tag="M2A35_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCB7200846.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRRAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVHYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            3334..3537
                     /gene="ydfZ"
                     /locus_tag="M2A35_004646"
     CDS             3334..3537
                     /gene="ydfZ"
                     /locus_tag="M2A35_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCB7200847.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3573..5033)
                     /locus_tag="M2A35_004647"
     CDS             complement(3573..5033)
                     /locus_tag="M2A35_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCB7200848.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDDSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5122..6405)
                     /gene="ydfJ"
                     /locus_tag="M2A35_004648"
                     /pseudo
     CDS             complement(5122..6405)
                     /gene="ydfJ"
                     /locus_tag="M2A35_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            7008..7121
                     /locus_tag="M2A35_004649"
     CDS             7008..7121
                     /locus_tag="M2A35_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200849.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            7190..7423
                     /gene="ydfK"
                     /locus_tag="M2A35_004650"
     CDS             7190..7423
                     /gene="ydfK"
                     /locus_tag="M2A35_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HCB7200850.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGNAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            7740..8330
                     /locus_tag="M2A35_004651"
     CDS             7740..8330
                     /locus_tag="M2A35_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7200851.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPSFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(8428..>8817)
                     /locus_tag="M2A35_004652"
     CDS             complement(8428..>8817)
                     /locus_tag="M2A35_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7200852.1"
                     /translation="SSWHLVEDHRGKTVYDVASGDALFISELGPLPKNVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEEISLL
                     EAWKKYRVLLNRVDTSTAQDIEWPALP"
BASE COUNT         2450 a         2183 c         1912 g         2272 t
ORIGIN      
        1 attcgacaaa atcacgcggc gtgttggtgc cggaaagcgt ggcataacgc tggcgggcaa
       61 aaaggccgtg cagcgtatga ccaaattcat ggaataaggt tattacatca tcccagagta
      121 acaacgcagg ctcaccggca gcgggtttct gataattgca aacgttataa attaccggat
      181 gcgtttcatt aagcgttgat tgctcaacaa aattgcccat ccatgcaccg ccgctttttg
      241 aatcacgggc gaagaaatca ccgtaaaata acgccagtcc cacgccatta tgatcaaaaa
      301 tttcccacac acgaacgtca ggatggtaga caggaatatc aaaacgttcg acaaacttaa
      361 taccgaagag ctgattcgcg gtccagaata caccttcatt caacaccgtg tttaattcaa
      421 aatatggctt gagctgcgcc tcatcaagat caaatttctc ccgccgtacc tgttcggcat
      481 aaaatgccca gtcccacggc tgcgcgctaa acccgccttg ctgcttatcg ataaccgcct
      541 gtatggaggc taattcatcg ctagcacgtt gacgcgccgc tggaacaatt tcccgcataa
      601 agttgagtgc tgcttcaggt gtttttgcca tctgatcggc gattttccat gcggcataat
      661 gaggaaaacc aagcagtttc gcctgctgtg cgcgaatctc taccagacgt tgaatgatag
      721 cgcgggtatc attgccatca tttttttccg ctcgcgtcca gcccgcaata aacagttttt
      781 cacgcgtcgc acgatcgcgc atttcggcaa gcgccggttg ttgggtggta ttcagcagcg
      841 gaatcagcca tttgttatcc agacctttct cgcgagccgc cactgccgcc agcgcaatct
      901 cttgctcact cattcctgcc agctgcgcga tatcgttcac aaccagaccg ccggatttat
      961 ttgctgcaag taaccgctgg ttaaactggc tggtcagggt cgcagcttct gtattcagta
     1021 cttttaattt tgctttatca gcttgcgcaa gtttggctcc ggcaaggaca aaacgttgat
     1081 gaataacctc caccaggcgg atggattcac tatcaagccc cagggattca cggcgctgcc
     1141 agacagcatc tacccgcgcg aataattcac cgttcagata gatatcatta gccagttccg
     1201 ccagttcagc ggaaaactgt tcatcaagac gctgtaattc atcattggta tgcgccgcag
     1261 tcatcgcaaa aaagacgctg gtaacgcggg taagtaattc tccgctttgt tccagtgcca
     1321 gaatagtatt gttgaaatca ggcgtttgcg ggttaagcgc gatggcagca atttctgccc
     1381 gcttttgctg cattccctca tcgaatgccg ggcgatagtg atgattggca atttgatcaa
     1441 aatgaggagc cagatacggc agtgtgcttt gcacaaggaa aggattcatt gttgtcattt
     1501 tcttctcctg aacgcgaggt gttccatagc gtaggcttac tgataacgta gtgcaatctt
     1561 gcaaaccagt attacccgct cttaagcatc acgtgctatg ttattgacac acaaaagcgt
     1621 tgaggaacag tgagatgatc gttttagtaa ctggagcaac ggcaggtttt ggtgaatgca
     1681 ttactcgtcg ttttattcaa caagggcata aagttatcgc cactggccgt cgccaggagc
     1741 ggttgcagga gttaaaagac gaactgggag ataatctgta tatcgcccaa ctggacgttc
     1801 gcaaccgcgc cgctattgaa gagatgctgg catcgcttcc tgccgagtgg tgcaatattg
     1861 atatcctggt aaataatgcc ggcttggcgt tgggcatgga gcctgcgcat aaagccagcg
     1921 ttgaagactg ggaaacgatg attgatacca acaacaaagg cctggtatat atgacgcgcg
     1981 ccgtcttacc gggtatggtt gaacgtaatc atggtcatat tattaacatt ggctcaacgg
     2041 caggtagctg gccgtatgcc ggtggtaacg tttacggtgc gacgaaagcg tttgttcgtc
     2101 agtttagcct gaatctgcgt acggatctgc atggtacggc ggtgcgcgtc accgacatcg
     2161 aaccgggtct ggtgggtggc accgagtttt ccaatgtccg ctttaaaggc gatgacggta
     2221 aagcggaaaa aacctatcaa aataccgttg cattgacgcc agaagatgtc agcgaagccg
     2281 tctggtgggt gtcaacgctg cctgctcacg tcaatatcaa taccctggaa atgatgccgg
     2341 ttacccaaag ctatgccgga ctgaatgtcc accgtcagta atttttatac ccggcgtaac
     2401 tgccgggtta ttgcttgtca caaaaaagtg gtagactcat gcagttaact cactcacaag
     2461 caagaacgaa tgaccgtcga aacgcaactt aatcccacac agcctgtcaa tcagcagatt
     2521 tatcgtattc ttcgtcgcga cattgtccat tgcctgattg caccaggcac accgttgtcg
     2581 gaaaaagaag tttctgttcg tttcaatgtg tcacgccagc cggttcgtga agcctttatt
     2641 aaactggcgg aaaacggcct gattcaaatt cgtccgcagc gtggcagcta cgtcaacaaa
     2701 atttccatgg cccaggtgcg aaacggcagt tttatccgtc gggccattga gtgcgcggtg
     2761 gcgcgtcggg cggcgagcat gattaccgaa agccagtgct accaactgga acaaaatctt
     2821 caccagcaac gcattgccat tgagcgcaag caactggatg atttttttga acttgatgac
     2881 aacttccatc aactcctgac gcagattgcc gactgtcaac tggcgtggga taccattgag
     2941 aacctgaaag cgaccgttga tcgcgtgcac tatatgagtt tcgaccatgt ttctccacca
     3001 gaaatgctgt tacgccagca tcttgatatt ttctctgccc tgcaaaaacg tgatggcgat
     3061 gcggtagaac gtgcaatgac gcaacatttg caggaaatca gcgaatccgt gcgccagatc
     3121 cgccaggaaa acagcgactg gtttagcgaa gagtaattca tttactctca tcccatccgg
     3181 gatgagaggc ttttcccccc gccttacggc tcatgcatgc atcaaaaaag atgtgagctt
     3241 gatcaaaaac aaaaaatatt tcactcgaca ggagtattta tattgcgccc gttacgtggg
     3301 cttcgactgt aaatcagaaa ggagaaaaca cctatgacga cctacgatcg taaccgtaac
     3361 gcaatcacca ctggcagccg tgttatggtt agcggcaccg gtcacactgg caagatcctg
     3421 tcgattgata ctgaaggtct gaccgctgag caaatccgcc gcggaaaaac cgtagttgtt
     3481 gaaggttgtg aagagaaact ggcacctctg gacctgattc gtctcggcat gaactaagtg
     3541 tgtgaatgcc gccgatggcg gcattgcttt ttttacttca cggaatattt cgccacggtc
     3601 gctttcgcgc catgcgctaa taaagacaag tacgcttccg tcacttttgc agtaaacaag
     3661 ctatcgtctg gcaaatcatc accaaagatt gccttaatcg ccagcaatga ctggacgcgc
     3721 gctttccctt cggcactact ttgtacagcc ttctgaataa caggtaacag tgggtcactg
     3781 atttctatcg gatttccctg ttcatcaaca ccaccgacat aacgcatcca acccgcgacg
     3841 cccagcgcca gcagatcgaa cttgctgtca tgcgccagat gccagcgaac agaatccaac
     3901 atccgctgtg gcaatttctg gctgccatcc atcgcaatct gccaggttcg atgacgtaac
     3961 gccgggttgc tatagcgtgc aattaatcgg ttagcgtaat cctgcaaatc aacgccctgc
     4021 actttcagcg tcggcgcttg ttcctgcaac atcaagccat acgccgcatg acgataatgt
     4081 tcatcttcca tgcagtcatt aatgtgctga tatccggcaa gataccccag atacgccagg
     4141 aatgaatgac tgccgttgag catgcgcaac ttcatctctt cataaggcag cacatcgcta
     4201 accagttcgg ctcccgcttt ttcccattcc ggacgtccgg caacaaagtt atcttctatt
     4261 acccactggc ggaaaggttc acaggcaacg cccgcaggat cgcgcacacc ggtaagttgt
     4321 tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa tacggtccac cattgttgat
     4381 gggaaagtca cgttatcttc gatccattgt gccagtttta catcaacggc ttgcgcgtag
     4441 gaagtgacaa catcacgcat aacatgaccg ttttctggca tgttgtcaca tgacatgacg
     4501 gtaaatgcgg gaagtcctgc cgctttacgg cgagccagcg cctcaacaat cacccctgtt
     4561 gctgttttcg gctggtgggg attttgcacg tcggcagcga ccatcgggtg atcgagcatt
     4621 aactgtccgg tcgccggaga gtggaaatac cctttttcgg tgattgtcag agagacaatc
     4681 gcgatttgcg gttcacacat cgcagccaac acggtttcta agccatccat ctgtacgtgc
     4741 aaggcttttt taacgacgcc aacgacgcga gccgtccacg catcggccga catttccgca
     4801 acggtataaa gattatcttg ctgttgtaaa tcggcaattt gctgttcgcc gccgattaag
     4861 ttgacctcat aatatcccca gtcactgaaa tgttctgtag caagaatatc ggcatacacg
     4921 ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa caattcttgg ggccaggtta
     4981 ttacgatcat aaacagggag tgtcgctttt gctgataaca aattatttcc cataacaatt
     5041 ccttaaattt aaatatagca agctacatgt tttgttatat gaataaaaat cccctctccg
     5101 gtaagagaag ggattgagga tttacagact tctggaaggt tgcgcagctc ttacaacacg
     5161 cggttgatct tccgcagcgt cttccagcgc acttaaatca cggtctttca cctctggcat
     5221 tttcagcgca gagattaaac caatcactga atatgccatg atcataatgg cgatcggata
     5281 ccaggattcc gtcatggtgc agaaaatacc cgccaggata ggaccaaaac cggaagcgat
     5341 aagaccacca atttctttag aaatagccat ccgggtaaag cggtttttac agccgaacat
     5401 ttctgccatg gtaatgtttt ccagagcaaa taatcccagc accgcacagt tatgaatcac
     5461 aatcagtgca accataatgg tgctcggggc atagctttta tctacaatga tagaaagcat
     5521 tggccatgcc agcacaatcg cggaggtatt cataataata tacgggatcc ggcgaccaat
     5581 tttatcggat aaccaaccaa ggaacggaat ggtcataaag ccgagaatcg aactaatcat
     5641 caatgcatct gttggaattg ctttgttaaa caataacgtc tgcactaaat agcctgcaag
     5701 gaaagtctga attaaacctg agttaccagc ctgaccaaaa cgcagccctg ttgccagcca
     5761 gaaggatttg ctctggaaca tgctaccagc aggtgcaggt tttgctgtcg gttggttgct
     5821 gtcattaacc ttctcaaaga ccgggctttc tttcagattc atacgcaacc agatagcaaa
     5881 gaccatcacg acaacgctcg ccaggaacgg tatacgccat ccccacgcca gcagttcctc
     5941 tttactgaga atgaagaaca taaaggccca gattgccgtt gcgctcaagg ttccgcagtt
     6001 agttcccata gctacaaatg aggagataat tccgcgctta ccttttggcg catattccgc
     6061 cagcatcgta ccggcaccgg aaatttccgc acctgcaccc aacccctgaa taatacgcag
     6121 cgtcaccagc aagatgggtg caaaaacacc aatctgtgca taggtcggta acacaccaat
     6181 taaggtggta cagatcccca tcatggtgat ggtaataaag agcacttttt tacgccctat
     6241 tctgtcgccc attttgccga aaataaatgc tccgacaata cgcgccacat aacctgcacc
     6301 gtaggttccc attgccagaa ttaacgccat tgccgttgat gattcaggaa aaaatatttc
     6361 atgaaacact aacgctgcgc cgagcgaata taactggaaa tccataattc acaggtgttt
     6421 tttcccatcc tgtggtttcc ttggcgtttt ctaggttttt tcagatagtt gcatttttta
     6481 aaaagcatcc taagttcgat ctcagtgtct atctggggcc tatttctgtc ccatatatgc
     6541 cccaaaaaaa ctccccaaca gataagtagt tttttcatgg atttatgcgt aaaatcaaga
     6601 acggctggaa atcattcaat actcacacta tcgaaaaatt taccagccaa ccgcagcacg
     6661 ttcttgcata aggcgtgtct gcggtttttc aactattcag atacatcact cccatcacat
     6721 tcattcctcc gcatcaaagg catataggct atatcacctt gatatttttc tttttcagat
     6781 aaaaactgtt atctatgtat acttttaaac ccaatccgtg tagagtctct gcataagata
     6841 gtttgcagtt gccgcttcag cttgcgccat aaccgcctga tttttgctgc cacctgttag
     6901 cattcctgta tacctgaaac gacaatgttt atctacgaac tttaagaaca cccaagataa
     6961 aaattgtcaa ctatatcata tataacacat tactaattcg aggctatatg aacagcatac
     7021 tgataatcac ttctctcctt atcatattca gcatttttag tcatgcccta ataaaattag
     7081 ggattggcat atccaataac ccagacaaaa ccgatgtata agtcaacata tcctgaatca
     7141 gacatacaat atcgcaatga aaatcaataa tattttaagg aatatcttca tgaaatcaaa
     7201 agacacccta aagtggttcc ctgcgcagct tcctgaagta agaattatcc tagggaatgc
     7261 tgtagtggaa gtagcaaaac agggaagacc tatcaatacc agaacattgc ttgattacat
     7321 tgaaggaaac ataaagaaaa aatcatggct ggataacaaa gaattattac aaacagcgat
     7381 atcagttctt aaagacaacc aaaatttaaa tggtaaaatg taatataata aacttacttt
     7441 tttatcattt ttccacttta acaacatttt gctccacttt tccacgacca aacaacttaa
     7501 aatctggtta aaataacacg aaacactatt cttcttcctt gagtccgccc ggaactcgaa
     7561 aaacaaaccg agttaaagcc atttttcaca aaatcgattt tgggtctcac caaaattacg
     7621 gggttgcata cgcattcgtt tattttcgaa cgtgtacata caaatatgca caaaaataat
     7681 caaaattatt ttctgcgatg cattatgata tgaacaccaa tttcgtatag agtctcacta
     7741 tgtctcgaat ttttgcttac tgtcggatat caacgctgga tcagaccacc gaaaatcaac
     7801 gccgggaaat cgaaagtgca ggttttaaaa tcaaacctca gcaaataatc gaagaacaca
     7861 ttagcggctc agcagcaacc agtgagcgtc ctagttttaa ccggttgctt gctcgcctga
     7921 aatgtggtga tcaattgatt gtgacaaaac tggatcgcct tggttgtaat gcaatggata
     7981 tcaggaaaac agtggaacaa ctgaccgaaa caggtatcag agtgcattgc ttagcattgg
     8041 ggggcattga cttgaccagt ccaacaggaa aaatgatgat gcaagtaatt tcagcagttg
     8101 ctgaatttga acgagacctt ttacttgaac gcactcattc cgggatagta agagcccgcg
     8161 gcgcagggaa acgttttggt cgaccacctg tgttaaatga agaacagaaa caggtggtat
     8221 tcgaacgaat taagtcaggt gtaagtataa gtgccattgc ccgggaattc aaaacctcgc
     8281 ggcaaaccat tttaagagcc aaagcaaaac ttcagacacc tgacatataa aaaataatct
     8341 cggtgtgaga tgctttacgt cttccaagcc cccttccttg ccgtaaatgg aaagatacat
     8401 ctaattaaag aatttatatg ttttacccta cggcagtgct ggccattcaa tatcctgtgc
     8461 agttgacgta tcaacacggt tcagcaatac ccgatacttt ttccatgctt ccagcaacga
     8521 gatttcttcc tccgttgcaa tttccagatc tgcagcatcc tgaagcggcg caatatgctc
     8581 actggctacc tgcatcaggt tgttttttgt ttcttccgcc tcccggatcc ggaacagttt
     8641 ttctgcttcc gtatccttca cccaggctgt gccgttccac ttctgaaact ccccttccgg
     8701 cgataaccag gtaacatttt tcggtaacgg accgagttca gaaataaata acgcgtcgcc
     8761 ggaagccacg tcataaaccg ttttaccccg atgatcttca acgagatgcc acgatga
//
LOCUS       DAHBHC010000134         8773 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHBHC010000134 DAHBHC010000000
VERSION     DAHBHC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.136"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(57..935)
                     /locus_tag="M2A35_004653"
     CDS             complement(57..935)
                     /locus_tag="M2A35_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCB7200853.1"
                     /translation="MKLKMQDLRLFNIVFESDPGWILDFSNRTLSAFFDEELNIDIDD
                     ERYQKEGTSKAKRVRCLLKQVDRETALRVLGALWQYKTESMPEQAEQSRNDYLALISR
                     LENADTDEAKGVKPVQAWHGVDWHSLIAEMNEMKSLPPHPRGFRFEAWLAELFSIFKL
                     APRSSFRNTGEQIDGSFRLNDEFYLMEAKWHQKRTSAADLHVFEGKLSTKATWTRGVF
                     ISWMGFTPEGLTAFGKGKRVICVSGYDLYHSLNHRIPLPDLLDAKTRHAAETGEPYAE
                     FDRLYALKDKQFGTLK"
     gene            complement(1263..1994)
                     /locus_tag="M2A35_004654"
     CDS             complement(1263..1994)
                     /locus_tag="M2A35_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCB7200854.1"
                     /translation="MNQPIHNAYWLSRFESILNGALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRKKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPVPGGCSDTRKDSDKKSGGSAVLWVKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGLGRLLDGR"
     gene            complement(3475..4605)
                     /locus_tag="M2A35_004655"
     CDS             complement(3475..4605)
                     /locus_tag="M2A35_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200855.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLVELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYPANSSGVSGYSDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGMVKRFPPIFGLLFYEQERLTGGVFRYVLIKF
                     YNAFKENLFFVEIISCCFLVEFFHDIDSFFINQRLSKLESFIDKWREGKEIWIKAYLQ
                     LIHNALGITETEKLVNLYNSFFTDKVVLWDESVKKVKWIYEK"
     gene            4790..5674
                     /locus_tag="M2A35_004656"
     CDS             4790..5674
                     /locus_tag="M2A35_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCB7200856.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSHICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPEVDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQALSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            5877..6557
                     /locus_tag="M2A35_004657"
     CDS             5877..6557
                     /locus_tag="M2A35_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCB7200857.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            6705..7382
                     /locus_tag="M2A35_004658"
     CDS             6705..7382
                     /locus_tag="M2A35_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200858.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGANGNATGA
                     SVEFIGK"
     gene            7412..7621
                     /locus_tag="M2A35_004659"
     CDS             7412..7621
                     /locus_tag="M2A35_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCB7200859.1"
                     /translation="MPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIRNAEGQLRW
                     RCWNFEPDAGKQLNSYLASEGILRQ"
     gene            7711..8529
                     /locus_tag="M2A35_004660"
     CDS             7711..8529
                     /locus_tag="M2A35_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCB7200860.1"
                     /translation="MQLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDRHTSRSERY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDKHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            8621..>8773
                     /locus_tag="M2A35_004661"
     CDS             8621..>8773
                     /locus_tag="M2A35_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCB7200861.1"
                     /translation="MTTSSHNSTTPSVSVTTASGNNQSQLVATPVPDEQRISFWPQHF
                     GLIPQWV"
BASE COUNT         2442 a         2168 c         1846 g         2317 t
ORIGIN      
        1 ccctgtacac gattctgtgt gaaacaacgg ggagcaggtc atcgcttccc aacccgttat
       61 ttcagcgtgc cgaactgttt gtctttcagt gcatatagtc ggtcgaactc agcatagggc
      121 tctcccgttt cagccgcgtg ccgggtttta gcatccagaa ggtcgggtaa agggatccta
      181 tgattcagtg agtggtaaag atcataacca ctgacacaga taacccgctt ccctttacca
      241 aaagccgtca ggccttcagg cgtaaagccc atccaactga taaaaacacc gcgtgtccag
      301 gttgccttgg tactgagctt tccttcaaat acatggaggt cggcagccga cgttcttttc
      361 tgatgccact ttgcctccat caggtaaaat tcatcattca gtcggaaact tccgtcaatc
      421 tgttcgcctg tatttcggaa agacgaacgg ggagcaagct taaagatact gaacagttca
      481 gcaagccagg cttcaaaccg aaaaccacgt ggatgtggcg gcagagattt catctcgttc
      541 atttcagcga taagtgaatg ccagtcaaca ccatgccatg cctggacagg cttaacgcct
      601 ttggcttcat cggtgtcagc gttttctaag cgggaaatca gtgcaagata gtcattacgg
      661 ctttgttcag cctgttcagg catggattcc gttttatact gccagagcgc accaagaacc
      721 cgcagagcgg tctcacgatc aacctgtttc agcaagcatc taactcgttt ggcttttgac
      781 gtcccctctt tctgataacg ttcatcatca atatcaatgt tcaattcttc atcgaaaaaa
      841 gcagacaagg tgcggttaga gaaatccagt atccagccag gatcgctctc gaacacaata
      901 ttaaacagcc tcaaatcctg cattttcagt ttcacgccta cctccttttt tcacagtata
      961 acgggatgaa agtgggggag gaaatgagtc agaacctgta caccagcatt aggggattcc
     1021 gcgtctggta caaagcagac tatgagaaga gagacgctat aacatttgag tcctaccaat
     1081 cgatctgttc atttctatga atggcagtaa atgcaaataa ccagaacaac caaaggtttt
     1141 tcgacttatg tgtcacgaac ggaagtagat ggcaatccat tgcattactt caggacgact
     1201 tttcagaaag cgagctaagg gcgttggcat aaactcaggc atcggatgct gttttacagg
     1261 gttcacctgc catccagtag acgccccagc ccacgactgc aaccataatt acgttgacca
     1321 gaaccgtcat gctgcttcgt ctcatacttc acgagataac gggctctctc catggctttc
     1381 tgcatcgctt ccacatcacc acgcttgacc cagagaacag cggatccacc agattttttg
     1441 tcagaatcct tccgggtatc agaacatcca ccaggcacag gcgtatcatc ggaagaaggg
     1501 cgagcatcag atgatgtcgg tttacggaaa ggcgtccgcc tggaaaaatg aaccagtcca
     1561 ttaccctgcc agggctcaag atgcagagca ctgcaccatg ccagttggat cagcgtcgcc
     1621 agcgaagaag gaacggtgaa atcccctggc gagcaccagg tatctttgtt gagcagtaat
     1681 atcacgtgat aatgtttcct gcctttttcc ttgccaaact cccgacacca gaaataacgc
     1741 agagtggttg cacgcacacg cttgttggca cgtcgttttt tccgctggta agcctgaatt
     1801 tttgctttca gggatgcaaa aaaacgagaa atcaccgctg aatctaaatc ggtatccatg
     1861 atggtggcag gcatatactc agggaaacgt aaatccaccc ggattaacga gacggcacgg
     1921 tgttgcgcca gggcaccgtt gagaatactt tcaaaacggg ataaccagta agcattgtgt
     1981 attggttgat tcatgatgag agatctccgt tgatttggat tgacttcaca tcattaatca
     2041 ccgcggtaaa actttacggc atccggcatt gagcaatgct acaactttct gattaagagt
     2101 gattgaaacg gaacaaaaag ataatcctac acacagggta aaaacactca agaacagcga
     2161 atggatttaa aaacgacatg gacaacgaga aaagtcagaa gggaaaagga tccacagaac
     2221 attcaaacaa aacaccagaa acgcaaccag aacgcttcct ggtaatgcaa atcaacaaca
     2281 acgccaaaac ctacagctta atcacacaac ttaatcacgg atgcgaatga catgtttaaa
     2341 acctgtttac agataacgcc gttacacgat catccccatc agaatcgatt ttcccttcct
     2401 gaaagcgtta cgtttcacta cgcaccataa aaacgattaa ccatcgataa cgtaaacttc
     2461 atctgaatca aaacaccaaa accgttcatc acactttcgc aatgcaagaa gagtcacatc
     2521 atgacgaaaa caatgacctt acacggaaaa gaaaaacgtc aggaatcgtt aatgcctaaa
     2581 tctgattgtt atcaaaacat ccagaaagtc gtttaacaga atcgtcgtaa gcacatcgaa
     2641 aagaacgtca gattacttca ggaaagttcg ggttgattta gcgtaaagat acttttctca
     2701 tgctaacgtc gaaactcgaa atatcaggaa aggttaaacg taacatcagt aaaatcgaat
     2761 agcagattga ttgctgaagc agaaaggttt tacagcagca ttaaactgat ttagttaacg
     2821 taaagattta ctaaactgga tgtgtatagc aatgcttaac gaaaacactt gtagaagtgt
     2881 ttatgaaaat acgtgtataa cacttactga aatgcttagt aaaaatacgt gtatatagta
     2941 tcataccgac attctcaact cttccaaaca tccatgcaga ctgaatgttg attacccgtt
     3001 ttcacatcag gcatggttct ttactgacca aaaaacaaac tggtttactc ttgaattatt
     3061 ttctctacct cagaaaacat ggtctcagga atctgttatg gcttcgtagt ttctgttgtt
     3121 cttttcataa aagttataag ttattgtcat aatcatacga taatcacttt atgttagttt
     3181 ttactgttac acgctactga ctttttcagt cattgccaga aaggggctct ttttacttcc
     3241 atttcaccac tcaacaaatg caaaactcgt ccagtaaagc ggaccaccac ttgctcacaa
     3301 gcaacgaatc gtctccagga acagccctca aacattgcag aaacaaaaac aacaaaaccc
     3361 tcattatcac tgatgttcgg aaaatttcca tgacccaaaa aacgaaattt aaatgagacg
     3421 cattatcatg cgtgtgatca aaaagacgtt atggtagctt tcagttaaat ggtgctattt
     3481 ctcataaatc cacttaactt tttttactga ttcatcccag agaaccactt tatcagtaaa
     3541 aaatgaatta tatagattga cgagtttttc tgtttcagtt attcccaatg cattatgtat
     3601 cagttgtaga tatgctttta tccagatttc tttaccctcc ctccacttat ctataaaact
     3661 ttccaactta cttaatcgtt gatttataaa aaatgaatca atatcatgaa aaaactcaac
     3721 aagaaaacag cacgagataa tctcaacaaa aaacaaattc tccttaaaag cattataaaa
     3781 ctttatcaac acataacgaa aaacacctcc tgttaacctt tcctgttcat aaaacaacaa
     3841 gccaaaaata ggcggaaatc ttttaaccat accactttta atccagtaag gatgatgatt
     3901 agtctttact ggcagtgaat aaaagaacaa ttcggtttct cttttcccac cacaaacaga
     3961 caaaacagtc ttataaagac tttttgaatc actatatcct gacactccag acgaattagc
     4021 agggtatttt ctttttactt tttgccttaa tggagctgga agaataataa aacgggatgg
     4081 tagataacaa tcgctttcag aagacctgca ttcgcacaca tgaagattta tatttacatc
     4141 cggacaattc aaaaaccaca cagaataatt atcgctccgt ttgcaatatg aaacccatga
     4201 ataaattgca taaagttcag aaagtacatc taactctgct tttgatttaa atgtgatacc
     4261 gtttcttcga attctcgttt ccagcgttct ccatgccgga gttttcagta tctcacgaac
     4321 ttttttatta agttcaacca gcgatggtcc ctgctcctgt ttatgtttct tcctgctgtt
     4381 aatgatttct ctttctttta ctaatttttt cttacttttg ggtcctttta tctttcttgg
     4441 caatgcttca tttaatctgt gaacaggatt ttgctctctt ctttttttga gctcacaaaa
     4501 cctctcataa tgttttctgc acagtctttt ctccactgcc atttttactg agaaaacctg
     4561 attgttacaa ttttttctga cgcatttgtt tttacgtccg gtcatcctct catctcctga
     4621 aaccgcagta tgtcattagt tcccgttctg aaaaactcac ttcatactcc attacacttt
     4681 aacaataata tccgtaattc agacattcca tcatttttca cgtccatatt acctgaatga
     4741 taaaccggat tagtgtgatc cggttcagtg aaatcaacag gataccggta tgtcattcag
     4801 ccattcttcc ctctccgcac aagtgaaatc atatctgacg tttcttcctg aagaaattcg
     4861 tcagaaaatt cttgaacatc tccacagcgt tattcattac gaacccgtga ttggcattat
     4921 gggtaaatcc ggcaccggca aaagtagcct gtgtaatgct atttttcagt cccatatctg
     4981 tgccacacat cccctgaacg gctgcacccg ccaggctcat cgtcttaccc tgcagatcgg
     5041 tgaacgcaga atgacgctgg tcgatctgcc cggcattgga gagacgccgc aacatgatca
     5101 ggaataccgg gcgctttacc gtcagttact gccggaagtg gatttgatta tctggatcct
     5161 gcgggcagat gaacgcgcgt atgccgccga cattaccatg catcagtttt tactgaatga
     5221 aggcgcagat ccctcgcgtt ttctgtttgt tctcagccat gccgatcgcg tgtttcctgc
     5281 tgaagaatgg aatgccacag aaaaatgccc gtcccgtcag caggcgctct cactggcgac
     5341 ggtgacagcc cgggtggcaa ccctgttccc ttcatcattt ccggtactct ccgttgctgc
     5401 acctgtcggc tggaaccttc cggcgtttgt gtcgctgatg atccacgcac tgccaccaca
     5461 ggcaaccagc gcggtgtatt cacatatccg gggagaaaac cgctctgaac aggcccagaa
     5521 acacgcacaa cagacttttg gtgaaaccat cgggaaaagt tttgacgtcg ccgttgcccg
     5581 tttcagtttt ccggcctgga tgttacagct tctgcgtaaa gcccgggacc gcattatcca
     5641 cctgctgatc acactgtggg agcgtctgtt ctgacacact cacgccgaca ggatgtgtcg
     5701 ctggattaac gagcattctt ctttttatga aatcatgctt aaaaatcaga taattaaaag
     5761 aatatttttt ctgctgcatt ttattcctga ttatccggat gcgacacatc ctttcaacat
     5821 catgatgcat aataacatca tgaaataaaa gatgttttct tacggagtgc acatctatgt
     5881 ctgataatcg ttcccggcat gatcgcctgg cggttcgctt atcactcatt atcagccgac
     5941 tgatggccgg agaatctctg tcactaaaaa cactgtcaga tgaatttggc gttacagaac
     6001 gtactttaca gcgcgatttt catcagcgtc tggttcacct agatttagag tacagaaatg
     6061 gcaggtacag cctcagacga cagagcagcc caggtgcgat ccctgaaatg ctttctttta
     6121 tacagaatac cgggatcgca cggatacttc cgctccggaa cggacgactg ataacctgtc
     6181 ttaccgacaa ccaggagccc tctccctgcc ttatctggct accggcgccg gatatcactg
     6241 caacgttccc cgagtgtttc tcgcaactca tcctggcaat aagacagtgt atccacatct
     6301 ctctgatgac tgagcgatgg tatccgtcac tggagccctg ccggctcatt tattacagcg
     6361 gtagctggta tctgatcgcg ttacagaagg gaaaactgca ggtctttcct ctggcagata
     6421 tcaaatcagt cagcctgaca tcagaacggt ttgaacggag aggccacatc cacagtctgg
     6481 tcgctgaaga gcgttttatc tccgccctgc cacatttctc tttcatccat aaacttatca
     6541 acacctttaa cctgtgattg ccggcctgcc aaagccgtcc cgacaggtat ggagacaata
     6601 tgttgaacag aaaactaaat atacggctac gtcattccct gaacagtcac tgcatacctt
     6661 ccatcattat caataacacc gtacgttcat ttcagaggtc agtcatgaat accagagctc
     6721 tttttcccct gctgttcact gtggcatcat tctccgcctc cgccggcaac tgggctgtca
     6781 aaaacggctg gtgtcagacc atgacggaag atggtcaggc gctggtaatg ctgaaaaatg
     6841 gcacgattgg tattaccggc ctgatgcagg gatgcccgaa tggtgtacag acgctcctgg
     6901 gcagccgtat cagtattaac ggtaacctga tccccacatc acaaatgtgt aatcagcaga
     6961 cgggattcag ggctgttgag gtggaaatcg gacaggcgcc ggaaatggtc aaaaaagccg
     7021 ttcactccat agcagagcgt gatgtgtccg ttttacaggc atttggtgta cgaatggaat
     7081 tcacccgcgg tgatatgctg aaggtctgtc cgaaatttgt cacatcactt gccggttttt
     7141 ccccgaaaca gacgaccact attaataaag attccgtcct gcaggctgcc cggcaggcat
     7201 acgcccggga atatgacgag gaaacaacag aaaccgctga ttttggctct tacgaagtaa
     7261 aaggcaataa ggttgagttt gaagtattca atcctgaaga ccgtgcgtac gacaaagtga
     7321 ccgtcacggt tggtgctaac ggtaatgcca ccggcgccag cgttgaattt atcggaaaat
     7381 agccggtatg taggactgcc accctgtttt attgcccgaa ggccctttct cacgcgaaca
     7441 ggcggttgcc gtcacaacag cttaccgcaa tgtgcttatt gaagatgacc agggaacgca
     7501 tttccggctg gttatccgca atgccgaagg gcagctacgc tggcggtgct ggaattttga
     7561 acctgatgcc ggaaaacagc taaattcgta tctcgccagt gagggaattc tcaggcaata
     7621 aacgtcttca tttcatccat caggccgcgt cttctccggg agacgcggcc ttttcgttta
     7681 taccgctaat tcattcataa ggagcaaagt atgcaattag ccagtcgttt tggttatgcg
     7741 aatcagatac gccgtgaccg tccgctgaca cacgaagaac tgatgcacta tgtacccggt
     7801 atttttgggg aagaccggca tacctcccgc agtgaacgct atacgtatat ccccaccatc
     7861 accgtgctgg aaagtctgca gcgggaaggc tttcagccgt tcttcgcctg ccagacccgt
     7921 gtgcgcgacc cgggccgccg gggatacacc aaacatatgc tgcgtctgcg gcgggccgga
     7981 gagataaacg gagaacatgt ccctgaaatt attctgctca actctcatga cggtacctcc
     8041 agctaccaga tgctgccggg ttacttcagg ttcgtctgcc agaacggatg cgtctgtggt
     8101 cagtctctgg gggaagtgcg tgttccgcac cggggaaatg tggtggagaa agttattgaa
     8161 ggggcttacg aggtggtggg ggtttttgac cggatagagg agaagcgtga tgccatgcag
     8221 tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
     8281 tatggtgaca aacatcagcc agtcaccact gccgacattc tgacaccacg acgccgggag
     8341 gattacggta aggacctgtg gagtgcttat cagaccatcc aggagaatat gctgaaaggc
     8401 ggaatttccg gtcgcagtgc cagaggaaaa cgtatccaca cccgtgccat tcacagcatc
     8461 gacaccgata ttaagctcaa ccgcgcattg tgggtgatgg cagaaacgct gctggagagc
     8521 atgcgctgat gctgtttccc tgaaagtgca gccctgactc acgtctgtac cagtccccag
     8581 gcattcttcc attcatttgc tttttataag gaataatctc atgacaacct cttcgcataa
     8641 ttccaccaca ccttctgttt ccgtaactac tgcatcaggg aataaccagt ctcaactggt
     8701 tgccactccc gtccctgatg aacagcgcat cagcttctgg ccgcagcact ttggcctcat
     8761 tccacagtgg gtg
//
LOCUS       DAHBHC010000135         8767 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHBHC010000135 DAHBHC010000000
VERSION     DAHBHC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.137"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..184)
                     /locus_tag="M2A35_004662"
     CDS             complement(<1..184)
                     /locus_tag="M2A35_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7200862.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLD"
     gene            281..700
                     /locus_tag="M2A35_004663"
     CDS             281..700
                     /locus_tag="M2A35_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200863.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRLKDNPPPE"
     gene            1007..1159
                     /locus_tag="M2A35_004664"
     CDS             1007..1159
                     /locus_tag="M2A35_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCB7200864.1"
                     /translation="MDTIDLGNNESLVCGVFPNQNGTFTAMTYTKSKSFKTEAGARRW
                     LAKNTS"
     gene            1568..1789
                     /gene="kilR"
                     /locus_tag="M2A35_004665"
     CDS             1568..1789
                     /gene="kilR"
                     /locus_tag="M2A35_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCB7200865.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            1783..1959
                     /locus_tag="M2A35_004666"
     CDS             1783..1959
                     /locus_tag="M2A35_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200866.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            2034..2309
                     /locus_tag="M2A35_004667"
     CDS             2034..2309
                     /locus_tag="M2A35_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200867.1"
                     /translation="MITSYEATVVTTDDIVHEVSLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            2410..5538
                     /gene="recE"
                     /locus_tag="M2A35_004668"
     CDS             2410..5538
                     /gene="recE"
                     /locus_tag="M2A35_004668"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCB7200868.1"
                     /translation="MSTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSAQGKPAGTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARAEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMNADYTDRGLLTKEWMKENRVSRITRTASCANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKHVEEIIAENKPPFSVFRDKFITMPGGLDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     EKQDDEEKLQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKYQKLRAE
                     LHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSVYQNQPETEQ
                     NDPNTQQNEPETKQPEPVVQQQETEKVCTACGKAGGGNCPDCGAVMGDATYQKTFNEE
                     NQNEYQEKGLEEMEGAEHPHKENAGSNPHCDCSDEAGEATAPVVTEIMWPSYFEPGRY
                     ENLPNEVYHSANGISSTMLKDARISLMYYHGRHIAGTILSEESDALLRGRIIHSYVLE
                     TDKFADEYAIPVPVPEYVVTTSNELIAIIKKHNASLPAPMTPEQMKEWIESYNSTLIQ
                     PLSVSAGAEEAGILYGSLPEEFRRIPEGEKHTASAMKACIKEYNANLPPLLKTSGSRE
                     QLLEQIETVDPELAKKERAKSLPYNISGTKEQLTEIARKIRPELVTLEDWQKRQQEEN
                     AGKTFISPDMYEQAKNIHAALQNNTDAARLLNHPDRKSEISYFGFDEETGLEIRVRPD
                     IEIRLPYESICADVKSVSLGYVRQERLKDRLHREIIERDYHLSAAMYCDVANLDKFFW
                     IFVNKDAGYHWVAVVEASQELLELGRQEYRRTLRQINEALETNNWPAPITESYTDELN
                     DFDLRRLEALSI"
     gene            5550..6602
                     /locus_tag="M2A35_004669"
     CDS             5550..6602
                     /locus_tag="M2A35_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCB7200869.1"
                     /translation="MNELTQQENINSNVAVFSPQSLAAIQTFSQVMASGMATVPEHLR
                     GNPSDCMAITMQAMQWQMNPYAVAQKTFVVNGVLGYEAQLVNAVISTRGPLTGRIEYD
                     WFGPWEKIIGKFEIRKNDKGKEYRVPGWKLADENGIGVRVQATLRGESKPRVLELLLA
                     QARTRNSTLWADDPRQQLAYLALKRWARLYCPEVILGVYTRDELDEPQEKIINPVQEH
                     KNTSACRAERETTIIEQDAGENWISAFRERIEQAQSTGETTALRQEVEDHKNTLGALY
                     TELKGKVVQRHHRLNAIARIEKMINDLPSSGDPEAEQKFIALENTLNAARPHLGELYE
                     AYKMTLTDMKPEYIGS"
     gene            6666..6860
                     /gene="ralR"
                     /locus_tag="M2A35_004670"
     CDS             6666..6860
                     /gene="ralR"
                     /locus_tag="M2A35_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HCB7200870.1"
                     /translation="MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            6853..7041
                     /locus_tag="M2A35_004671"
     CDS             6853..7041
                     /locus_tag="M2A35_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HCB7200871.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKTKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            7148..7429
                     /locus_tag="M2A35_004672"
     CDS             7148..7429
                     /locus_tag="M2A35_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCB7200872.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            7395..8471
                     /locus_tag="M2A35_004673"
     CDS             7395..8471
                     /locus_tag="M2A35_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCB7200873.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         2763 a         1925 c         2072 g         2007 t
ORIGIN      
        1 cgtcaagtcc acaatgagcg ccaagccgat taagggtaga aacaattttt ctgcactcct
       61 ctagtccagg ggtacgaaaa tttgcttcgt aatttgccag tcggctttgt atccacccta
      121 actgaacagc aagttgtctt tgagacagcc caagctgttt tcgatatgtt gaaattttgt
      181 tcattgaaaa cctccgatga caattttaaa cacatcttgt gttatatggt caagctgttt
      241 tgtgttttat gtaaatcacg attcgtgata caaggatgca atggaaaaag aaaacgaaaa
      301 aattgccgct agtaggctca atgacaaaat tgcaatgcgt cttaaagagc gcaggcagaa
      361 gcttggttta tctcaaggaa aacttgctga aatctgcgga tggacgcaat cgcgtatagg
      421 taactatgag gcgggcagca gaaatgttgg agtgcatgac gctgtcgtat tgggaaaggc
      481 tcttggcata tctcctcctg agctcctctt tggagaacag gaatcttctg aattgtggtt
      541 aaatgaatcc caacgaaaac ttcttgagtt gtttaaccag ctaccgggct cagaacaaca
      601 acgaatgatt gagctatttg aagtccggct aaaagaaatc gatgagtatg tagaaaaata
      661 tttgagaggc aggcttaaag ataatccccc accggagtaa tgatcttgct atcacagtaa
      721 tatgccaatc agcccgctat cagcgggctt tttttgtacc atcatcatat gacacccacc
      781 acaaaacaca tttcgtgttg acacaagaaa acacattgtg tttaataagc atatccaaac
      841 aacgccccac cagagaacgg caggacaata cctcgagtta tccagccact gaacagggct
      901 aagtagccag cctgaggcat acgaacatga cggcagttgt tgattgatac aaagcgcagt
      961 agataaaacg ttccgccacc cggcgttaag gggaaatgag gtcaacatgg atactatcga
     1021 tcttggcaac aacgaatctc tggtatgtgg cgtgtttccc aatcagaacg gcacattcac
     1081 cgccatgacg tataccaaaa gcaaatcgtt taaaaccgaa gctggcgcac gtcgctggct
     1141 tgccaaaaat accagctaaa ccatttattg gattaattca atattcttgc tgtaggggta
     1201 tagccgaggc caccaaagcc cggaggtggt gaaataaaac cgggcacaac acgaaggcgc
     1261 atttccggta tccataaaga gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc
     1321 tccggttgta aataacgaca ttgctatgtg tagtctttgg cagcatcagt tctactccgt
     1381 ggctgccctg ccgccccttt ttaaagtgaa ttttgtgatg cggtgaatgc ggctaagcgc
     1441 acgcggcaca gttaaaagca tcagtgttat gggtggatta tccggcgtta attgttaact
     1501 ggttaacgtc acctggaggc accaggcacc gcatcgacaa aattcatttg taaaaatgga
     1561 gataattatg attgctcatc acttcggaac tgatgaaata ccacgtcagt gtgtgactcc
     1621 tggcgattat gttcttcatg aaggtcggac atatatcgcc tcggcaaaca atattaaaaa
     1681 gcgaaaactt tatattcgta acctgactac aaaaacatgc atttctgact gcatgattaa
     1741 agtcttcctc ggtcgtgatg gtttacctgt aaaggcggag tcatggtgat gactaagaaa
     1801 ataaaatgtg cttatcacct ttgcaataaa gaaattgaag aaagcaaaat cattacaaga
     1861 ccacttcatt tcatgcgtgg agttatacca acgacggaaa tgaaaaaata ttgtagtgaa
     1921 atctgtgccg aaaaagacca gatggcacac gaactttaat taactgacta tccgaaactg
     1981 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgatta
     2041 ccagttatga agccactgtt gttactactg atgacattgt tcacgaagtc agcctggaag
     2101 gaaagcgtat tggctacgtg attaaaacag aaaataaaga aacccctttc actgtggttg
     2161 atatcgacgg tccatcaggc aacgttaaaa cgcttaacga aggcgtcaaa aaaatgtgcc
     2221 tggtgcatat cggaaagaat ctgcccgcag aaaaaaaagc cgaatttctg gcaactctga
     2281 ttgcaatgaa attaaaaggt gaaatctgaa agaaatagcc tgcgtatggc gcaggctatg
     2341 aacagtgtgt atccggcaag attattcact gaacaaacga attttaatct gagttgaggt
     2401 taaaaaacaa tgagcaccga taaacaagtt tacccactgt attacgaagc aaaaaatgac
     2461 aaagtaagaa aacgtctcgg tattaaaggc ggtttttact gggctgaagc gaaaaaatta
     2521 tccattgcca tctcccgtgg tgctgttgcg attgacgatg ctggctacga tgaagatgac
     2581 ttcaaaaaac ctgttcgcgt caatttgccc gttgttgatg acctcccgcc agaaggcgta
     2641 tttgatacgg aattctgcaa ccgttacgaa aaaggcgggg aagatggcat cacaatggta
     2701 tttatcgcgc cctcaccctc tgcgcagggc aaaccagccg gcactgacaa caccaatgtt
     2761 aatggcgaag acatgacgga gattgaggag aatatgctac tcccgatttc tggccaggag
     2821 ctgcccattc gctggcttgc tcaacacggc agcgaaaaac cggtaacgca cgtttcacgc
     2881 gacgaactcc aggcattaca cattgcacgg gctgaggaac taccagctgt tactgccctg
     2941 gctgtttccc acaaaaccag cctgctcgat ccgctggaaa ttcgcgatct ccacaaactg
     3001 gtgcgtgata ctgacagagt tttccctaat ccaggcaatt caagcctggg actgatgact
     3061 gcttttttcg aagcatacat gaacgcagac tacaccgatc gcggtctgct gacaaaagag
     3121 tggatgaaag aaaatcgtgt ttcacgcatc acgcgcacgg cttcctgcgc taatgctggc
     3181 ggcgggaacc tcaccgatcg cggcgaaggt ttcgttcacg atctgacgtc actggcgcgc
     3241 gacgtagcca ctggcgtact ggctcgttca atggacgtgg acatttataa ccttcatccg
     3301 gcacacgcta aacatgtcga ggaaattatc gctgaaaata aaccgccctt ttctgttttc
     3361 cgcgacaaat tcatcaccat gcctggcggg ctggattatt cccgcgccat cgtggttgcg
     3421 tccgtaaaag aagcaccaat tgggatcgag gttatccccg cacacgtcac tgaatatctg
     3481 aacaaagtac tgactgaaac tgatcatgcc aaccctgatc cggaaatcgt ggatattgcc
     3541 tgcggtcgtt cctctgcccc gatgccgcag cgtgtaacag aagaagaaaa acaggatgat
     3601 gaagaaaaac tgcaaccatc ttgcgcaatg gcagatgaac aggcaacggc tgaaacagtg
     3661 gaaccggatg caactgaaca tcatcaggac acgcagccgc tggatgctca gtcacaggta
     3721 aattctgttg atgcgaaata tcagaaactg cgggcagaac tccatgaagc ccggaaaaac
     3781 attccgccca aaaatcctgt cgatgcagat aaattactgg ctgcctctcg cggagaattt
     3841 gttgaaggga ttagcgaccc gaatgatccg aaatgggtta aggggatcca gacccgcgac
     3901 tccgtgtacc agaatcagcc agaaacggaa cagaacgacc caaatacgca acaaaacgag
     3961 ccagaaacga aacagcctga gccagtagtg caacaacagg aaacggagaa agtttgcacc
     4021 gcctgcggta aggctggcgg cggcaactgt cctgactgtg gcgcggtgat gggcgacgca
     4081 acgtatcaga aaacctttaa tgaagaaaat cagaatgaat atcaggaaaa aggtctggag
     4141 gaaatggaag gtgctgaaca tccgcacaag gagaacgctg gcagcaatcc gcattgcgat
     4201 tgcagtgatg aagctggtga agcgacagca cctgtagtaa ctgaaatcat gtggccgtca
     4261 tatttcgagc ctggccgcta tgaaaacctc ccgaacgagg tttatcactc cgccaacgga
     4321 ataagcagca caatgctgaa ggatgcccgc atcagcctga tgtattacca cggacggcac
     4381 attgccggaa ctattctgag cgaggaaagt gatgcattgc tgcgtgggcg gattattcac
     4441 agctatgttc tggaaacgga taaattcgct gatgagtatg ccattccggt accggttcct
     4501 gaatatgtgg ttactacttc taacgaactg atcgccatca ttaaaaaaca caatgccagt
     4561 ctgccagcac cgatgacacc agagcagatg aaagagtgga tcgaaagcta caacagcact
     4621 cttatacagc cactgtcggt aagtgccggg gccgaagaag caggcatcct ttacggttcg
     4681 cttccggagg aattccggcg tattcctgag ggggaaaaac acacagcatc agcaatgaaa
     4741 gcctgtatta aagaatacaa cgcaaacctc cctcctctgt tgaaaaccag tggatcacgg
     4801 gagcaacttc tggagcaaat tgaaactgta gatccagaac tggcaaaaaa agaacgtgct
     4861 aaatctttgc cttacaacat cagtggcaca aaagagcaat taaccgaaat cgcacggaaa
     4921 attcgcccgg aactggtgac actggaagac tggcaaaaac gccagcaaga agaaaacgcc
     4981 gggaaaacgt ttatcagtcc ggatatgtat gaacaggcaa aaaatattca cgctgcactg
     5041 caaaacaata ccgatgcagc aaggctactc aaccacccgg atcgcaaatc tgaaatcagc
     5101 tatttcgggt ttgatgaaga aaccgggctg gaaatcaggg tccgtcctga tatcgaaatc
     5161 cggctgccat acgaaagcat ttgcgccgac gtgaagtcag tcagcctcgg ttatgtgcga
     5221 caggaacgac tgaaagatcg cctgcaccgt gaaattattg agcgtgatta tcacctcagc
     5281 gcagcaatgt attgcgatgt ggcaaacctg gacaaatttt tctggatctt cgtcaacaaa
     5341 gatgctggct atcactgggt ggccgtcgtg gaagcctcgc aggaactcct ggaacttggt
     5401 cgacaggaat atcgccggac gctacgacag ataaatgaag ctctggagac aaacaactgg
     5461 ccagcaccga ttaccgaaag ttataccgac gaattaaacg actttgatct tcgtcgtctt
     5521 gaagcactga gcatctgagg aaggacacaa tgaacgaatt aactcaacaa gaaaatatta
     5581 actctaatgt tgcggttttc agccctcagt ccctggctgc aattcagaca ttttcccagg
     5641 taatggcttc cggcatggct actgtaccgg aacacctccg gggaaatcca tcagactgca
     5701 tggccatcac catgcaggcg atgcagtggc aaatgaaccc ttacgcagta gctcagaaaa
     5761 ctttcgttgt gaatggtgtg ctcggatatg aagcgcaact ggttaatgcc gtaatcagta
     5821 ctcgtgggcc gctaaccggg cgtattgaat atgactggtt cgggccgtgg gaaaaaatta
     5881 tcgggaaatt tgaaatcagg aagaacgaca aggggaaaga atatcgtgtg cctggctgga
     5941 agctggccga tgaaaacggg atcggtgttc gcgtccaggc aacactacgc ggagagagca
     6001 agccacgcgt actggagtta cttctggcgc aggccagaac acgtaactca acgttatggg
     6061 ccgatgatcc tcgccagcag cttgcctatc tggctctgaa acgctgggcg cgcctttatt
     6121 gccctgaagt gattcttgga gtgtacaccc gggacgaact ggacgaacca caggaaaaaa
     6181 tcattaatcc ggttcaggaa cataaaaaca catccgcctg ccgtgcggaa cgtgaaacaa
     6241 caattattga gcaggatgcc ggggaaaact ggatcagtgc tttccgtgaa cgtattgagc
     6301 aggcacaaag caccggagaa acaacggcac ttcgccagga agtggaagat cataaaaata
     6361 cacttggcgc tctctacaca gaacttaaag gaaaagtggt tcagcgtcat caccgtctca
     6421 atgctattgc ccgtattgag aagatgataa atgacctgcc ttcatcaggt gatccagaag
     6481 cagaacaaaa atttattgct ctggaaaata cgctgaatgc tgcacgacca catctgggag
     6541 aattatatga ggcgtataaa atgacactga cagatatgaa accagaatat atcggctcct
     6601 gatatttact atggcggtgt agcctcaccg ccataacaaa actttatttt atgagagaaa
     6661 agacaatgcg gtatgaaaaa gtcaaaccat gtccgttttg tggttgtcca tcagtaacgg
     6721 tgaaagccat ttcaggatat taccgcgcaa agtgtaacgg atgcgaatcc cgaactggct
     6781 atagtggaag tgaaaaagaa gcactcgaaa gatggaataa acgaactact ggaaataata
     6841 atggaggtgt tcatgtataa aattactgcc actattgaaa aggaaggtgg cactcctact
     6901 aactggacaa ggtactcaaa aacaaaatta accaaatcag aatgcgaaaa aatgctctca
     6961 gggaaaaaag aagcaggcgt ttccagagag cagaaagtaa agctgataaa ttttaattgc
     7021 gagaagcttc tgtcctcgtg agttgcatta tatacaaatt agaacttcat agctgattat
     7081 taaaaatcaa ccacacccgc cagtattctg tatatttact ggcggtcata tcgtaagagg
     7141 tatggcaatg aatcttgtga cactcaaaac gtggggaaaa ctcagatatc cggataaccc
     7201 accatcaata tcaacgctga gacgatgggc aaggaatgga aacatttatc ctgcacctga
     7261 actacacggg aggagttaca gagtggttcc ggaggctttc tatatcaacc caaataaggt
     7321 tgataccgat ataacacacc atcagcctaa tgggcgacaa gggagagaca gtccgttact
     7381 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaaa aacttaacat
     7441 atcgaaggag ggacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
     7501 taggtaaaat ttcaagaaga gatgcagtag cgcaggcaat tgaagcaaat cattatatat
     7561 acaaaaacta ctctcctgct gccttaattg aaaagcttaa agggttcgac tcatttacta
     7621 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
     7681 cttataaaat tcgggtaaat caactggaga caataaaaga aaaattggga gggattttac
     7741 tgacagaaat aacaactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
     7801 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
     7861 ttgaaggacg tatatctcaa aatccagtaa cgccaacaag agcaccgaaa atagtagtta
     7921 caagagaacg actgaaacta aagacataca actgcatcag ggaggcagca gatcaacttc
     7981 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
     8041 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacatcagg caaattaaga
     8101 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
     8161 gtacagtagt tgaacggtgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
     8221 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
     8281 cagccagaaa attaacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
     8341 gaagtctggc tggacgattg tacaaagaaa catgtggaga agaatttgcc cagcgcctac
     8401 ttggtcacac atcggagaag acaacaaaaa tgtatcttga tgagcgagaa aaaacgtact
     8461 tactgctctg attttaacgt aaatggattg ttaaatgtgt tttggttgtg atataaccaa
     8521 aaaagaccgg aatacagaaa ttcgagtaaa tttcggggaa tttcgggaag acgtttgcaa
     8581 ctgattgatt ttaaatacaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     8641 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     8701 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     8761 taaaaag
//
LOCUS       DAHBHC010000136         8660 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHBHC010000136 DAHBHC010000000
VERSION     DAHBHC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.138"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(112..330)
                     /locus_tag="M2A35_004674"
     CDS             complement(112..330)
                     /locus_tag="M2A35_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200874.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLFSLEQDITNLEKAAALSIARMITYPR"
     gene            complement(490..645)
                     /locus_tag="M2A35_004675"
     CDS             complement(490..645)
                     /locus_tag="M2A35_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCB7200875.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            complement(814..1221)
                     /locus_tag="M2A35_004676"
     CDS             complement(814..1221)
                     /locus_tag="M2A35_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7200876.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            1302..1529
                     /locus_tag="M2A35_004677"
     CDS             1302..1529
                     /locus_tag="M2A35_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7200877.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            1513..2034
                     /locus_tag="M2A35_004678"
     CDS             1513..2034
                     /locus_tag="M2A35_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200878.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            2015..2980
                     /locus_tag="M2A35_004679"
     CDS             2015..2980
                     /locus_tag="M2A35_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200879.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIDSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            3021..3443
                     /locus_tag="M2A35_004680"
     CDS             3021..3443
                     /locus_tag="M2A35_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCB7200880.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            3440..3796
                     /locus_tag="M2A35_004681"
     CDS             3440..3796
                     /locus_tag="M2A35_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCB7200881.1"
                     /translation="MSAPATILDMCCGSRMFWFDKSDERAIFSDIRKEGYTLRNGRRL
                     IINPDIIADFRALSFADASFSMVVLDPPHLERVGDNAWMGKKYGRLNKDAWRDDLRQR
                     FKEAFRVLRPHGVLIF"
     gene            4407..4694
                     /locus_tag="M2A35_004682"
                     /pseudo
     CDS             4407..4694
                     /locus_tag="M2A35_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5463..6776
                     /locus_tag="M2A35_004683"
     CDS             5463..6776
                     /locus_tag="M2A35_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCB7200882.1"
                     /translation="MIKETVTMSHKELHRLQIIQEQAAARIGISIRQVKRLVQRYRNE
                     GPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREIHGLSLSVET
                     LRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRGPRCTLIVFI
                     DDATSALMALRFVPAETTRAYMETLRGYLNDHGVPLALYSDRHSIFRVNNPEREGELT
                     QFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIETANAWLPTF
                     IEAYNNRFATSPRTTDNAHLDVHHSEEELGYIFSLQAKRVLSKNLTFQYKSSAFQVRS
                     EGRGYRLRHSVVTVCENFDGEINVLYDGKALGWEKYVDGPEPIPLDDEKSVHERVDNA
                     RIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKKD"
     gene            complement(7213..7545)
                     /locus_tag="M2A35_004684"
     CDS             complement(7213..7545)
                     /locus_tag="M2A35_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FlxA"
                     /protein_id="HCB7200883.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(7748..8053)
                     /locus_tag="M2A35_004685"
     CDS             complement(7748..8053)
                     /locus_tag="M2A35_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200884.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            8078..8317
                     /gene="relB"
                     /locus_tag="M2A35_004686"
     CDS             8078..8317
                     /gene="relB"
                     /locus_tag="M2A35_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCB7200885.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            8317..8604
                     /gene="relE"
                     /locus_tag="M2A35_004687"
     CDS             8317..8604
                     /gene="relE"
                     /locus_tag="M2A35_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCB7200886.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT         2362 a         1795 c         2178 g         2325 t
ORIGIN      
        1 atcctttcag taaactgtca gtgccggatg ctcacccgtg tccggcgcac gcactccacc
       61 tcacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct attaacgcgg
      121 gtatgtaatc attctggcaa tgcttaatgc cgctgctttt tccagattgg tgatatcctg
      181 ctccagagag aacagatttt cagcctgctt agccctggct tcattagccc atttcagatc
      241 ctgcgctgca ttaattttct ggtgcatcca ctcataaagt tcatcatcgg tatagtctgg
      301 cgcgatgata acgggttctc gtttctgcat actgattcct cgcggtgctg tttcgcttat
      361 cagccgttag attttgccga actggaaagc gcctgtttaa attcgctgaa gctgagagct
      421 tcttcgcctt cggcaaggcc ttcgaagtat tcttcgtaag ccttttccat gattgtgtcg
      481 aaatccatat cacccacctg aatttctttc cagccagcga cgcgctccag attcggtttt
      541 aaacgttttg cttttggtat acgtcattgc ggtgaacgtg ccgtcctggt ttggaaacac
      601 gccgtacacc agagattcgt tgttgccaag atcgatagta tccatgctga cctcatttcc
      661 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatggaaa
      721 ataatttaga ataacctaac atgagaggca agtgtttttt gttagattga tctaacaaaa
      781 agggtgggcg caactaatca cttgaaaaga atgttatttt attgatttat ttttacgcgc
      841 tttaagcatt tcttcgaaga gtttgttgaa gttttctact cttgcgcgca tttcagacag
      901 caaggcttcc tgctcggaag atggaagagc atcgaataat tcgatcaatt ctttgtggtt
      961 gggagttagc tctgtttcca catgaagttc ttgtgcaggc actggtgcct tgtcttcgtc
     1021 accaaacatt agccatgtag gtgagcactt cagagcatcc gctaaagcaa acaatcgttt
     1081 tccgactggc tgggtttcgt ctctttccca ttgtgaaatt gtgacgtgag caactccagc
     1141 gaggcgcgcg gcttctcgtt gtgttaagcg taattctttt cgtcgcgcca gaactcgctg
     1201 gcccagggtt cttgtatcca tagttaggta attctaattt ttcttgactt aggtatcccg
     1261 cgcacaataa tgttagaaaa gtctaacaag agggggattt gatgcttaaa gttgacgcaa
     1321 ttactttttt tggcagcaaa acaaagcttg ccaatgccgc aggagtgagg ctggcaagtg
     1381 ttgctgcttg ggggatactg gttcctgaag gtcgcgcgat gcgtctacag gaggcatctg
     1441 gcggggagct tcagtatgat cccaaagttt atgatgaata tcgtaagacg aagcgggcgg
     1501 ggcggttgaa caatgaaaat cactcctgaa caggctcgtg aggctctgga tgcctggata
     1561 tgtcgaccag gaatgacaca ggagcaggcg acgatattaa tcactgaagc attctgggct
     1621 ttgaaagagc gcccgaacat cgatgttcag cgtgtcacag atgaaggtgg cgcggttgat
     1681 cagcgagcgc ttggtgttaa tcgagtgaag atattcgaac gctggaaggc tatcgacacc
     1741 agggataagc gtgaaaagtt cacggcgcta gtgcctgcga ttatggaggc tatccggatt
     1801 agtgatttca ggttgtatcg tgaaattact gacggaaaaa gcatcacgtg catgatcgcc
     1861 gggttaaaca aagaatatgg cgatgtggtg gagtccggac tgctttttgc tgatcctgcc
     1921 gtagtggatc gtgaaactga cgaacttata gaaaaagcaa ttgctttcaa gcttgcgtat
     1981 cgacagcaat accaacaaaa agctggatgg aattatgagc cttctttttg ctgaacgccc
     2041 actggttata aacacgcagc tggcaatgaa aattggctta aacgaagcca ttgttttgca
     2101 acaactgcac tactggttga gagataccaa ctccggcatg gaatgtgatg gtgttcgctg
     2161 gatttataac acaacggaac aatggctgga acagttccca ttctggtcag agtcaacgtt
     2221 aaagcgcgcg tttgcaagtc tgaaaacgct ggggcttttg cgttgtgaaa agctcaataa
     2281 atcaaagcgc gatatgacca atttctacac gatcaactat gggagcgagc ttttagatgg
     2341 tggcaaattg agcgaatcca tcggttcaaa atgcgccgct ccatcaggtc aaaatgacac
     2401 gatggaagag gtcaaaatga aacgctccat tgattcaaaa cgacccaatg tcatcgggtc
     2461 aaaatggcct gatgatctta cagagaatac aacagagatt actacagaga ataaaaacac
     2521 ttttcgtccg gaagcttcgc aaccggaccc gcagacgact gaacaggatt ttttaacccg
     2581 gaactccgac gcggttgtgt ttagtgcgaa aaaacgccag tggggtagca gggaggattt
     2641 ggcgtgtgcg cagtggatct gggggcggat cgtgggtctc tacgaacagg cagccagtga
     2701 tgatggcgag atcatgcgac caaaagagcc taactggact gcctgggcca atgacgtgcg
     2761 cacaatgcgg atgctggatg gcagaagcca cagacaaatt tgcgaaatgt ttggtcgggt
     2821 tcagcgagat ccattctggg taaaaaacat catgagcccg tcaaagctcc gcgaaaaatg
     2881 ggacgaactg gtcatccgcc tgggacgttc acctgtacag cgttgtgtta atcatatttc
     2941 tgaaccggat acagaaattc cgcctggttt cagaggataa gtgttgattt caggtcatga
     3001 ggtaatttta agggggactt gtggcaaaag tttttacaca agaagagcgg gaaaaaatta
     3061 aatggcaggt ggtggaactc gtgcgccaga gcggccgtga gacgttacgg caactggaag
     3121 ctaaaacagg tgcgactaga tatctgatga gcgttcttgc cagagagctg gtagccagtg
     3181 gcgatgtata caactccggc tacgggttat tcccctctga acaggcgcgt aaggactggc
     3241 aaaacgcccg caaaaaactc tcgagggcaa aggtgaagaa acctgctgtg gttgatccgg
     3301 accttatctg gtcatcacct gacggagaaa tacgtcgcta cgacagtcgc ctaaacataa
     3361 tctgtcgcga gtgccggaag agcgaagtta tgcagcgcat actggctttc tatcagggta
     3421 attttcagga cgtggcgcag tgagtgcacc ggcaaccatt cttgatatgt gctgtggcag
     3481 ccgcatgttc tggttcgata agtctgacga acgggcgata ttcagcgata tcagaaaaga
     3541 aggatacaca ttacgcaatg ggagacgctt gattatcaac cctgacatta tcgcagattt
     3601 tcgtgcatta tcatttgcag acgcatcttt ttcgatggtt gtattagacc ctccgcatct
     3661 tgagcgtgtt ggtgataacg cctggatggg aaagaaatat ggacggctga ataaagatgc
     3721 ctggcgtgat gatttgcgac agagatttaa agaagccttt cgggtgttga ggccgcacgg
     3781 cgttctgatt ttttaatgga atgaaacgca aataccggta agccagattc tggcactgac
     3841 agacagaaaa cctgttatct gtcaacgaac agggaaaaaa cgataaaacc cactggatta
     3901 tttttatgaa agaggcatcc agtgagtaga ttcgtaaggc tacagatacg tatatctgaa
     3961 taattaaatt cagttctgta aataaaattt aatccttaac cggatggatt tctgcaccct
     4021 cagaacatca ggaggccgcc cgaaagggcg gtagtgaaat gcgaaagttc aaaattatta
     4081 ttgaaacggg aatagctggt ggagattttg aggatgtatt cgaagtggac gatgacgcaa
     4141 cacctgatga aattcatgac ggagcataag aaattttctt taactactgc aattactcat
     4201 accacgaaat aaaagacgat gaggaagaac aaagtggctg atttttgttc agctaaatat
     4261 aacatcagtt ttgaagagcg ggatgaacta ttaatggact atggtgagtt acgcggtgga
     4321 agtgctgctg atgtcgaatc ctagcgtgat gactatgaag cgggaaaaac tccggtcgaa
     4381 gcatattgtg atgagtgggg cgatgaatga gcgagattaa ttatcaggaa gggcatgaaa
     4441 cggcggggca agcaaaacca gtgacatggc gatatcgcta cgtgaaaaaa ggcgttacgg
     4501 actttcaggg gaagtagtgg tctggtgact ggaaatatgt accgaaaaaa gaggattgta
     4561 acgacaggcc gaactatcaa attcaggcct tattcactgc ctcaccagtc ccggttacat
     4621 cagaagaatt ggttaaagct gtgcactttt atgaacaact aaaacgcgaa aatccaccaa
     4681 catccggcaa ctagattaat gggttaacta tgtcggttaa acgaccagcc aactgaaaaa
     4741 gcggaaacct gattacaggt tgccagataa ggcaatgagc tacctggcgc ggaacggact
     4801 gataagtatg gggaatgttt tacgatgaac ctttagacta aagagtttgt aacgctatgt
     4861 aagtgatttt ttctggttta gatatttata tgtccggcca aattgaggtg tgtttaaatg
     4921 taattgcaca ttgattgtag gaggaataat gaaaaacgca ttgcagtttt tgtttgttgc
     4981 gttctggttg ttcgcatcat gtatgcccat catcttcaca gcaaggtata tggaaaaagt
     5041 tgatgttttg atattaatat ttggatatat aaatgccctt tttttagggg tgttcatggc
     5101 ggtcatgtgc attgaatact ggcggtaaat acagcgaact ccattggttt agttggatat
     5161 ttactgtgct ggacaaaaac ggtttgcggg gaaatcttag ttaagtagaa tgactgcggg
     5221 tgcttgaggc tatctgcctc gggcatggac accaacggca gatagagaaa agccccagtt
     5281 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gccaatttca tgctagacac
     5341 atgtaggtta gcctcttacg tgccgaaagg cacggagaag caggctattg ttaacaccaa
     5401 gctgtaatgt cccctttgaa ccattctaaa atgtccccag acaattctct gggggatttt
     5461 tcatgatcaa agagactgtt acgatgagtc ataaggaact ccaccgactt cagattattc
     5521 aggaacaagc tgcggcacgc attggcattt ctattcggca ggttaaacgt ctggtgcaac
     5581 ggtatagaaa tgaagggcct tctggtctgg tttcccaccg acgtggaaag cgtcctaata
     5641 attccttttc tactgaattc agagcaacag taatttcact cctcaaaggc cgttacgctg
     5701 attttggacc tacgtttgcg tgcgaaaaat tgcgcgagat acacggttta tctttatccg
     5761 ttgaaactct cagaaagtgg atgatagaag aggggttatg gcgtgaacgc cgtcgtaaaa
     5821 ttgcccgtat atatcaacgc cgcatgcgac gaccatctta cggtgaactg atccagattg
     5881 atggctcacc tcatgactgg tttgaaaatc gaggccccag atgtacactg atcgttttca
     5941 ttgatgatgc caccagtgcg ttgatggcgt tgcgttttgt gcctgctgaa acaacccggg
     6001 cttacatgga aaccctccgg ggttacctta atgatcatgg cgtaccgctc gctctctact
     6061 ctgatagaca cagtatattc agggtaaata acccagagcg ggaaggtgag ctgacccagt
     6121 tcactcgtgc gataaagaca ctgggcatcg agccaatcca tgccaacagc ccgcaggcaa
     6181 aagggcgggt agagcgcgcc aatcagacac tacaggacag gctggtcaaa gaaatgcggc
     6241 ttcagaatat cagtgatatt gaaacagcaa atgcatggtt gccgaccttt attgaagcct
     6301 ataacaaccg gttcgctacg tcgcctcgta ctactgataa tgctcatctt gatgtgcacc
     6361 attctgaaga ggaactgggt tatatcttca gcctacaggc gaagcgcgtt ctgtctaaaa
     6421 atctcacttt ccagtacaaa agcagtgcgt ttcaggtacg cagtgagggc cggggatatc
     6481 gacttaggca ttcggttgtt actgtatgcg agaactttga cggtgaaatt aacgttctgt
     6541 atgacgggaa agcgctgggc tgggaaaagt atgttgatgg cccggagcct ataccactgg
     6601 atgatgaaaa gagtgtccat gaacgagtgg ataatgcccg tattgattta cgctcaaaat
     6661 actatgttaa acctaaagct gaccatccct ggcttacgcg ccgaacgcaa agtcatcagc
     6721 aagttaagcc cccgaagtta cctaaaaaga agcctgatcc cgataaaaaa gattgaaacc
     6781 aagatcgatt cggttgagtg catatccatt catagggtag attcttaagt cgcgtttctg
     6841 gtgttcattt tcgggtggtt tgttacttgt tttaccgggg atatgccaga aacgcgctga
     6901 gtcagtctgg gcggtgcgcg taatgaggcg ttatggtaaa tagcctatgc taatgtccgc
     6961 taagagcaag aagcggaagt tggcagtttt gtggactgtc cccacaaaag tgactacaga
     7021 aatagttgca attcataatt gatcatgggt tgtcagttaa actcgtggcg atttaaatag
     7081 actaattggg agtgcgtcca ttacttatat cttgtaatgt taactatcag aaatgataca
     7141 aagataatat gtctttaaag aaaaggctga tggcgaaaag tggcccgatg agggccacaa
     7201 tacggctgtc acttagacgt aaatatcaat ggtgccagcg gtatttgtat cgtctttttt
     7261 ctcttctttt ttatcaggct gaactgtcgc gtcttcattc tttttctctg cctgctgcct
     7321 taacaactgc tccagttgag cccagaggct ttcaatttgc ttctgtacca atgcagccat
     7381 ttcttttttc tgctgtgtcg tcatcccctc ttccgatgag attttcccaa gcttttcagt
     7441 cagcacctga atttgtcttg tgattttggc tatttctgat gttccttccg gggcggagtt
     7501 gtttgaaata acggttgagg tatttccctg aattgtgaca gacatagatt tctcctttta
     7561 aaaaagcact atcggcatgc acaaaaaaat ctttaatcgt atttcttgtg tcattaattg
     7621 tttgatgttc agattgtttt cctcgcgggc tggcgcgcct cagaaagtaa agcttgttga
     7681 caggggtaaa cgttcggcaa taattttctg ccgcatgcgg gtgttgcata aaacgtgtta
     7741 cgttccttta tcgacaggtc aggtcaccgc tcacccgccg acgagaaagc aatactgaca
     7801 tgctaaagca aaaaatagat gaataagttg agttgtgcat atgtagcctg accgtcacaa
     7861 agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaacg agctgacctg
     7921 cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca gtgatcaccg
     7981 ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg tagtgcgata
     8041 cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta acctgcgtat
     8101 tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg taactccttc
     8161 tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc cgttcaaaca
     8221 gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac ggcttcgtaa
     8281 tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct ggattttgac
     8341 gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca gttgaaaaag
     8401 aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg tggtatgcct
     8461 gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca ggttatagac
     8521 gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga agtatatagc
     8581 gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc accgaaggtg
     8641 acacttctgc tttgcgttga
//
LOCUS       DAHBHC010000137         8019 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHBHC010000137 DAHBHC010000000
VERSION     DAHBHC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.140"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            490..612
                     /locus_tag="M2A35_004688"
                     /pseudo
     CDS             490..612
                     /locus_tag="M2A35_004688"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            679..5962
                     /locus_tag="M2A35_004689"
                     /pseudo
     CDS             679..5962
                     /locus_tag="M2A35_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            6171..6437
                     /gene="hipB"
                     /locus_tag="M2A35_004690"
     CDS             6171..6437
                     /gene="hipB"
                     /locus_tag="M2A35_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCB7200887.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6437..7759
                     /gene="hipA"
                     /locus_tag="M2A35_004691"
     CDS             6437..7759
                     /gene="hipA"
                     /locus_tag="M2A35_004691"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCB7200888.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
BASE COUNT         2197 a         1701 c         2177 g         1944 t
ORIGIN      
        1 tgggttatag tttcattgac agccacatca tgatgaccgt ggctgttagt ttttgagtat
       61 agtttttctt atttcccttc ttttagagct ggcttgttaa atgccagctt gcagattcat
      121 cgttccatca ccagtactat attcaaggga aaattgctat ttttcttaaa gcattaagca
      181 taacccttat tttatagcga atattcctaa agacattaat ggatgcaaaa ggggtttaat
      241 aaacttcgat ttttagcaat tagtgctatt ctttgttaaa tatgatgatg ataattttcc
      301 tattgaaaga aacagttttc ttattaatca acaataactc atagttcatt tttaaataaa
      361 ataagaacta tgcatttttt aatctatcaa ttcgaaagta ttagcaaatt agccaaattg
      421 acaccttccc acctccaact aacacttaat acagacctct gacaatttaa taagtttcat
      481 ttttaatgtc tgacgactca tctctttcgt ggctgtattg tttgtatatg gagcgtttat
      541 atgaatagaa tctatcgcgt gatatggaat tgcactctac aggtatttca ggcctgctcg
      601 gaattaactc gcagggcagg taaaacatcg acggttaatt tgcgtaaatc ctctggactg
      661 acaacgaaat tcagtagatt gacgctgggt gttttgctgg cactaagcgg ttcagcgtct
      721 ggtgcaagtc tggaagttga taatgatcag attaccaata ttgatactga tgttgcttat
      781 gatgcctacc tggttggctg gtatggcact ggagtgctta atattttggc tggcggtaat
      841 gcctccttaa ccactattac taccagcgtc attggcgcta atgaggactc agagggcacc
      901 gttaatgttt tgggtggcac ctggcgattg tatgatagcg gaaataatgc aaggccttta
      961 aatgtgggtc aatccggaac ggggacgctg aatattaaac agaagggtca cgtcgatgga
     1021 ggctatttaa gattaagttc ttcgacagga ggcgtcggga cggtcaatgt tgagggagag
     1081 gactctgttc tgacgaccga attattcgaa atagggagtt acggtacggg ctcattaaat
     1141 attacggata agggttacgt cacgagttca atcgtcgcca ttttaggcta tcaggcgggc
     1201 agtaatggcc aggttgtcgt tgaaaagggt ggcgagtggc taataaaaaa taacgattcc
     1261 tcaattgaat ttcaaattgg taatcaagga actggggagg cgactattcg cgagggtgga
     1321 ctgattacgg ctgaaaatac gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat
     1381 gtgcaggatc aagactctgt catcacggta cgcagactct ataatggata tttcggtaat
     1441 ggcacactca atatttccaa taatggacta attaataaca aagaatattc attagtgggc
     1501 gttcaggacg gttcccacgg tgtcgtcaac gtgaccgata aagggcattg gaatttcctc
     1561 ggaacgggcg aagctttccg ctatatctat atcggtgatg ctggcgacgg tgaacttaat
     1621 gtctcgcgtg aaggtaaagt agattcggga attatcactg cggggatgaa agaaacaggc
     1681 acaggcaaca ttactgttaa ggataagaac tccgttatca ctaatctcgg aactaatctt
     1741 ggttatgacg gccacggcga aatgaatatc agtaatgaag ggcttgttgt cagcaacgga
     1801 ggaagctcac tcggttatgg agaaaccggc gtcgggaatg tcagcatcac cacggggggg
     1861 gatgtgggag gtcaataaga atgtctatac caccattggt gttgcgggcg tcggaaacct
     1921 caatattagc gatggcggta agttcgtatc gcaaaatatt acttttttgg gcgataaagc
     1981 aagcggtatc ggcacactga acctgatgga tgcgacatca tcgttcgata ctgtgggtat
     2041 caatgtcggt aattttggta gcggtatcgt aaatgtcagt aatggtgcca cccttaattc
     2101 aacgggctat ggatttatcg gaggaaatgc ctccggtaag gggatagtta atatttcaac
     2161 ggacagtctc tggaatttaa agacatcatc tactaacgca caattgctac aggtcggtgt
     2221 attaggcaag ggtgaactga atattaccac cggaggtata gttaaagcgc gtgatacaca
     2281 gatagctctc aatgacaaaa gtaagggcga cgtgagggtg gatgggcaga actctcttct
     2341 tgaaacattc aatatgtacg tagggacatc tggtacgggt acgttaaccc tgacgaatag
     2401 cggcacgctg aatgtcgaag gtggagaagt ttacttaggt gtttttgaac ctgctgtagg
     2461 aacgctaaac attggtgcag ctcacggtga ggcggcggca gatgccgggt atataaccaa
     2521 cgcgacgaaa gtagagtttg gttctggcga aggtgttttt gtctttaatc atactaataa
     2581 cagtgatgcc ggctaccagg tcgatatgct gattacaggt gacgataaag acggaaaagt
     2641 gatccatgat gcaggccata cggtgttcaa tgcagggaat acttatagcg gtaaaacgct
     2701 ggtcaatgac ggcctcctga ccattgcgtc tcatacggca gatggggtaa cgggcatggg
     2761 ttcgagtgaa gtcaccattg caagccccgg tacgctcgac attctcgcat caacgaacag
     2821 tgcaggagat tacacgctga ccaatgcgct caaaggcgat ggcttgatgc gagtgcagct
     2881 gtcatcctac gacaagatgt ttggctttac ccatgcaaca gggactgaat tcgccggtgt
     2941 tgcccaactg aaagacagta ccttcactct ggaacgcgac aacaccgctg cgcttactca
     3001 cgcgatgttg cagtctgaca gtgaaaatac cacatcggta aaagtgggag agcaatccat
     3061 tggtggactg gccatgaatg gcggtacgct cattttcgat acggatattc ctgctgcgac
     3121 gcttgcagag ggatatatca gcgtcgatac gctggttgtc ggcgcgggtg actacacctg
     3181 gaaaggccgt aactatcagg taaacgggac gggcgacgtg cttatcgacg tgcctaaacc
     3241 gtggaatgat cccatggcga ataaccctct gacgacgctc aatttgctgg aacacgacga
     3301 tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg gtggctcatt
     3361 aacgttacgt gatttacagg gcgacgaggt ggaagcggac aaaacgttac acattgcgca
     3421 aaacggaacg gtggtcgccg agggtgatta tggattccgc ctcacgaccg caccaggtga
     3481 tggtttgtac gttaactatg ggctgaaagc gctgaacatc catggtgggc aaaagctgac
     3541 gttagccgaa catggcggag cctatggcgc aacggccgat atgtcggcaa aaatcggtgg
     3601 tgaaggggat ctggcaatca atacggtgcg acaggtttcg ctttccaacg gtcagaacga
     3661 ctatcagggg gcaacctacg ttcagatggg gacattacgt accgatgcgg atggtgcact
     3721 tggcaacacc cgggaactga acatcagcaa cgcggccatc gtcgatctta atggatcgac
     3781 gcagacggta gagacattca ccgggcagat gggttcgact gttttgttca aagagggggc
     3841 gctgacggta aataaaggtg ggatcagtca gggtgaactg acaggtggcg gaaacctgaa
     3901 tgttacaggg ggaacgatgg ctatcgaggg gcttaatgca cgctacaatg cgttaaccag
     3961 cattagccca aatgcggaag tcagcctcga taatactcag gggttaggca gaggaaatat
     4021 tgccaatgac ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgta atagcataag
     4081 cgggaagggt atcgtgagcg caaccgccag gacagatgta gagctggatg gcgataatag
     4141 ccgctttgtg gggcaattca acattgatac aggcagcgcg ctcagcgtca acgagcagaa
     4201 aaacctgggt gatgcttccg ttatcaataa tggcctgctc accatctcca ctgagcgtag
     4261 ctgggcgatg acgcacagta tcagcggtag cggtgatgtg acaaaactgg gtaccgggat
     4321 cctgactctt aacaacgatt ccgcggcgta tcagggtacg acggatatcg tgggggggga
     4381 aattgctttc ggttccgact ctgccattaa tatggcaagt caacacatta atatccataa
     4441 cagcggtgtg atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta tgcctggggg
     4501 gacactgcgt gtcgctaaaa ccacaatcgg cggcaacctg gagaatggcg gcacggttca
     4561 aatgaatagc gaagggggga aaccggggaa tgtactgacc gttaacggca actataccgg
     4621 aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc gataattcgc ccaccgataa
     4681 aatgaacgtg aaaggcgata cccaagggaa cactcgcgtt cgggttgata acattggcgg
     4741 cgtcggtgca caaacggtca acggtattga actcattgag gttggcggta attctgcagg
     4801 taacttcgcg ctgaccaccg gaactgtcga agctggggct tacgtctaca cgctggctaa
     4861 agggaagggg aatgacgaga aaaactggta tctgaccagt aaatgggacg gcgtaacgcc
     4921 accggatacg cccgacccca tcaataatcc ccctgttgtg gatccggaag gcccatcagt
     4981 ttatcgcccg gaggccggaa gctatatcag caacattgcc gcagccaact cgctgtttag
     5041 ccatcgttta cacgaccgtc tgggtgagcc gcagtatata gattcactgc attctcaggg
     5101 gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa gggccggtga
     5161 cggtcagcta aatactcagg ctaaccgcta tgtattgcag ctaggcggcg atttggcgca
     5221 gtggagtagc aacgcgcagg atcgctggca tcttggcgtg atggcaggtt acgctaatca
     5281 gcacagtaat actcagagta atcgtgtggg ttataaatcg gatgggcgca tcagcggtta
     5341 cagcgctggg ctgtacgcga cctggtatca gaacgatgcg aataagaccg gcgcttatgt
     5401 tgacagctgg gcgctgtata actggtttga taacagcgtc agttccgata accgttctgc
     5461 tgacgactat gattctcgcg gtgtgacggc ctctgttgag ggtgggtata cctttgaagc
     5521 gggaacattt agcggcagcg aagagacgct gaatacctgg tacgtccagc cacaggcgca
     5581 aatcacctgg atgggtgtga aagattccga ccatacccgg aaagacggaa cgcgcattga
     5641 aacggaaggc gacggaaatg tgcaaacgcg acttggggtg aaaacctacc tgaacagcca
     5701 tcaccagcgt gacgatggta aacagcgtga gttccagcct tacattgaag cgaattggat
     5761 caacaacagc aaagtctacg ccgtgaagat gaatggtcaa accgtaggcc gtgaaggtgc
     5821 gcgtaatctc ggtgaagtac gtaccggggt tgaggcgaaa gtaaataaca accttagcct
     5881 gtgggggaat gtcggtgtgc aactaggtga taaaggctat agcgatactc agggcatgct
     5941 gggagtgaaa tatagctggt aaaccgtata agccgcatgt cgagatggca tgcggcttaa
     6001 tattgccgac ttcaaaggct ttaaagcctc ccttttatcc gcgatcgcgg atatcgcagc
     6061 gtttatcccg tacagcggat aagatgtgtt tccagattga ctttatcctc actaaaggat
     6121 aaaactgata atatcccctt aagcggataa atttgctgtg gacgtatgac atgatgagct
     6181 ttcagaagat ctatagccca acgcaattgg cgaatgcaat gaaactggtt cgccagcaaa
     6241 atggctggac gcagagcgag ctggcgaaaa aaattggcat taagcaggcg acaatttcca
     6301 atttcgaaaa caaccctgac aataccacgc tcacgacatt ttttaagatt ttacagtcgc
     6361 ttgaactctc aatgacacta tgcgacacga aaaatgcctc gccagaatca acagaacagc
     6421 aagatctgga gtggtaatgc ctaaacttgt cacttggatg aacaaccagc gggtaggcga
     6481 gttaacgaag ttagccaacg gcgcgcacac ctttaagtat gcaccggagt ggttagccag
     6541 ccgttatgcc agaccgttgt cactttcgct gccattgcag agggggaata tcacctcaga
     6601 tgccgtattt aacttcttcg ataacctgtt acccgatagc ccgattgtac gtgaccggat
     6661 cgttaaacgt tatcatgcta aatccagaca accgtttgat ttattgtcag aaatagggcg
     6721 agacagcgtt ggtgccgtaa cgttattacc cgaaaatgaa accataacgc gcccgataat
     6781 ggcatgggaa aagcttactg aagccaggct tgaagaagta ttaacggctt ataaagcaga
     6841 tatccctctg ggcatgatta gagaagaaaa tgactttcgc atctcggttg ctggcgcgca
     6901 ggagaagaca gcgctgctta gaataggcaa tgactggtgc attccgaaag gaataacgcc
     6961 gacgacgcac atcattaaat taccgattgg cgaaatcagg cagcccaatg cgacgctcga
     7021 tctcagccaa agcgttgata atgagtatta ctgtctgctg ctggcgaaag aacttgggtt
     7081 gaatgttccg gacgcagaaa tcattaaagc gggaagggtg cgcgcgttag cggtcgaacg
     7141 ttttgacagg cgttggaata ctgagcgaac ggttttactt cgcttgccac aggaggatat
     7201 gtgtcagaca ttcggtttac cttcatctgt gaaatatgaa tcagatggag gcccaggcat
     7261 cgcgcggatc atggcgtttt tgatggggtc cagcgaggcg cttcgcgatc gttatgattt
     7321 tatgaaattc caggtcttcc agtggttgat tggcgcaacg gatggccatg caaaaaactt
     7381 ctccgtattt attcaggctg gcggcagtta tcgactcacg ccattttacg acatcatttc
     7441 agcatttccg gtccttggcg gtacgggaat acacatcagc gatctcaaac tggcaatggg
     7501 gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc gacatttttt
     7561 ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc taagtgactt
     7621 tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga cagattttcc
     7681 ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac ggttaagccg
     7741 agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcac gacaatcatg
     7801 accgccaacg actggattct tccttggttt atactggtgc gcataatgtg acacgcgtta
     7861 tgttgaaaag gccgctgcga tactctccac cgcagcggcc tttttacaca taacgtcatt
     7921 gtgcgcactg tgtcattttc tgttgtgtat tggcgtatct gtgacgccag cacgctgaag
     7981 aagaagaaaa gggaaggaga acgtcgtcac gccagccgc
//
LOCUS       DAHBHC010000138         7981 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHBHC010000138 DAHBHC010000000
VERSION     DAHBHC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.141"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..135
                     /gene="hokE"
                     /locus_tag="M2A35_004692"
     CDS             <1..135
                     /gene="hokE"
                     /locus_tag="M2A35_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCB7200889.1"
                     /translation="LVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVLAYEPKK
                     "
     gene            complement(257..877)
                     /gene="entD"
                     /locus_tag="M2A35_004693"
     CDS             complement(257..877)
                     /gene="entD"
                     /locus_tag="M2A35_004693"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCB7200890.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1052..3292)
                     /gene="fepA"
                     /locus_tag="M2A35_004694"
     CDS             complement(1052..3292)
                     /gene="fepA"
                     /locus_tag="M2A35_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCB7200891.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYVTTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3535..4737
                     /gene="fes"
                     /locus_tag="M2A35_004695"
     CDS             3535..4737
                     /gene="fes"
                     /locus_tag="M2A35_004695"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCB7200892.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4740..4958
                     /gene="ybdZ"
                     /locus_tag="M2A35_004696"
     CDS             4740..4958
                     /gene="ybdZ"
                     /locus_tag="M2A35_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCB7200893.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            4955..>7981
                     /locus_tag="M2A35_004697"
     CDS             4955..>7981
                     /locus_tag="M2A35_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HCB7200894.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADAHYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLA"
BASE COUNT         1713 a         2148 c         2181 g         1939 t
ORIGIN      
        1 cttgtggcag tcatagtgct gtgtttgacg gtactgggat ttacgcttct ggtcggagac
       61 tcgctgtgtg agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tctcgcttac
      121 gaaccgaaga agtagccgtt gtgcggggag caatccccgc atatccggtt gtcaggtcag
      181 agtggtaagg cacctgcttt acactttcgc ccgcggtcag tgatggctgc gggcgaatcg
      241 taccagatgt tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct
      301 gccagtgcac agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc
      361 taattatctg atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc
      421 tctctttggc ggaaaatgcc agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga
      481 gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag
      541 aaaaaatttc ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag
      601 ccccgcagtg gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc
      661 cgattgcggg cacacattta tagccatatt cccgcagcgc ataaattgca gcgatccgcc
      721 cggctaaatg ttcggctttg cgcttccgtc cagcgtgttg cagttgcgcg taatgcggca
      781 gccagagtaa atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat
      841 gtccggcaaa ggggagggag gtatgcgtag ttttcatgtc gaccatatta gagactgatg
      901 acaaacgcta aactgcctga tgcgctacgc ttatcaggcc tacatggtcc tgcaatatat
      961 tgaattggca agatttttgt aggccggata aggcgttcac gccgcatccg gcatgaacga
     1021 cgcgcacttt gtcaacaatc tgacgttagc atcagaagtg agtgtttacg ctcatgtacc
     1081 acgtacgtcc cggctcgtta taggtatacg ccccggcacc cgcgatatag ttggcccctg
     1141 ccagatcgcc cgtggtctgg gcattacccg cacgccacaa acgtttgtcg aacaggttgt
     1201 ccacgccgcc ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa
     1261 tgctgtaagg actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt
     1321 acttcttcgg ctgctgctta ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttctc
     1381 gtgcctgcca gctcagcgtt gagttcaacg tatactccgg gatgatcgac aaacggtcgc
     1441 ccgtggtttt gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt
     1501 cgctaaccgg tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat
     1561 cccactgata gagatcggtg ccgactgcgt tttgtcctac agccacatag cccgcttcaa
     1621 tcttattgcg ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga
     1681 actccagacc aatctctttg ttgatgctgg tttctgcttt caggtcatcg ttaccttgca
     1741 gatagcagcc gcccgcgctg gcatagcaac cctgaccttt actgtagaga atgtagttcg
     1801 ggttagtctg gtacaggctc ggtgctttat aagcacgggc aatgcccatt ttgagcgtga
     1861 agtcatcgcc taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac
     1921 tgtgatgatc gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt
     1981 tttcggcaaa cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca
     2041 caccatcaat agcgccgccg gtgttcgccc cggtcagcgc ctgggtattg gaactcaagt
     2101 ccttcatccg ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat
     2161 caatcggcag gttaacttcg ctgtgcagca tcacgtcatc aagatcgata tcgacgaaat
     2221 cctgtgtcgc tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac
     2281 gcgagttacg ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc
     2341 caccgttcca ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt
     2401 tcgagcgagt gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttacctt
     2461 ggcggctgta acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca
     2521 cgccattaat atctttgttg atcacccctt cgcgcccggc tggtaacgtc gtgacatacg
     2581 ttccggcacg cgcggactga tggccctggt tgatatccca cgcgtcagcc tgggttttgt
     2641 cgaggttgcc atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctgaagt
     2701 tggtacgttt ggtggcacct tcctctttat gttctggcgc attgaaataa gcatcccagg
     2761 agccgtgcca ctcaccactg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc
     2821 cgttgccata acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag
     2881 gtggcaccca ggaggtatca ccacgggtat cgcgctcacc acgccagccc tgacgcaccg
     2941 aattacggct gcttactggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatgc
     3001 cgcgaatatc aatctggcgg ttattaccac gctgaccact ggtggagtta ccggtcaggt
     3061 taacgcctgg catggtacgg atgatctccg acacatcgcg ggcaactggg tttttgcgga
     3121 tttcatctgc ggtgatggtc gaaacgccag gtgcctgcaa gttctgctcg gcggcggtaa
     3181 cgacaatagt atcgtcatgt gaaacaggag tatcggtcgg ctcttgtgcc tgcgctaccc
     3241 cataaatccc cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat
     3301 tcctgcattt ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaat acgacgcatc
     3361 ccgctaccgc gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc
     3421 cgcgcttccc acggcgcgcc attacgctat tgcaaatgca aatagttatc aataatatta
     3481 tcaatatatt tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg
     3541 gcgttaaaag taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt
     3601 ctgaatgacg aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa
     3661 tactcgacga taaagcgcgt atgggtctac atcactggtg tgaccgatca ccatcagaac
     3721 agccagcccc agtcgatgca gcgaattgca ggcaccaacg tctggcagtg gacgacacaa
     3781 ctcaatgcca actggcgcgg cagctactgt tttattccca ccgaacgcga tgacattttt
     3841 tctgcaccat cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag
     3901 gcaatagccg atccgctgaa tccacaaagc tggaaaggcg ggcgagggca cgctgtttct
     3961 gcactcgaaa tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa
     4021 acgcctgcca aagaaattat ctggaaaagt gaacggttga aaaattcacg ccgtgtatgg
     4081 atttttacca ctggcgatgc aacagcagga gaacgcccgc tggcagtttt gctcgatggc
     4141 gaattttggg cgcagagtat gcctgtctgg cctgtgctga cttcgttgac ccatcgtcag
     4201 caacttcctc ccgccgtgta tgtgttgatc gacgctatcg acactacgca ccgcgctcac
     4261 gaactgccgt gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg
     4321 aaagctattg ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt
     4381 ggtggacttt ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta
     4441 agccagtcag gatcgtactg gtggccgcat cggggcgggc agcaagaagg tgttttactt
     4501 gagcaactta aagctggtca agttagtgcc gaaggtctgc gcattgtgct ggaagcgggt
     4561 attcgcgagc cgatgataat gcgggccaat caggcgctgt atgcgcaatt acaccccata
     4621 aaagaatcca ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt
     4681 ggcttgatgc aggggctaat cgacctctgg caaccacttt tccatgacag gagttgaata
     4741 tggcattcag taatcccttc gatgatccgc agggagcgtt ttacattttg cgcaatgcgc
     4801 aggggcaatt cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt
     4861 gtcagccgca gtcacaggcg tcctgccagc agtggctgga agtccactgg cgtactctga
     4921 caccggccaa tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc
     4981 cgcacagccc ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt
     5041 ggcgcattac gttgagttaa ctggagatgt tgatgcgcca ttactggccc gcgcggtggt
     5101 tgtcggacta gcgcaagcag atacgttgcg gatgcgtttt acggaagata acggcgaagt
     5161 ctggcagtgg gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac
     5221 caatattgat ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt
     5281 gcgcgtcgat agcggtaaac cactggtctt tcatcagctg atacatgttg cggataaccg
     5341 ctggtactgg tatcagcgtt atcaccattt gctggtcgat ggcttcagtt tcccggccat
     5401 tactcgccag atcgccaata tttactgcac atggctgcgt ggcgaaccaa cgcctgcttc
     5461 gccgtttacg cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc
     5521 ctggcagcgt gatgcggcat tctgggcaga acagcgtcgt caactgccgc cgcccgcgtc
     5581 actttctccg gcacctttac cggggcgcag cgcctcggca gatattctgc gcctgaaact
     5641 ggaatttacc gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac
     5701 cgatttagcc cttgcgctgg cagccttgtg gctggggcga ttgtgcaatc gtatggacta
     5761 cgccgccgga tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc
     5821 cgtgctcaac gttttgccgt tgggtattca cattgcggcg caagaaacgc tgccggaact
     5881 ggcaacccga ctggcagcac aactgaaaaa aatgcgtcgt catcaacgtt acgatgccga
     5941 acaaattgtc cgtgacagcg ggcgagcggc aggtgatgaa ccgctgtttg gtccggtact
     6001 caatatcaag gtatttgatt accaactgga tattcctgat gttcaggcgc aaacccatac
     6061 cctggcaacc ggtccggtta atgaccttga actggccctg ttcccggatg tacacggtga
     6121 tttgagtatt gagatcctcg ccaataaaca gcgttacgct gagccaacgt taatccagca
     6181 tgctgaacgc ctgaaaatgc tgattgccca gttcgccgca gatccggcgc tgttgtgcgg
     6241 tgatgtcgat attatgctgc caggtgaata cgcgcagctg gcgcagatca acgccactca
     6301 ggttgagatt ccagaaacca cgcttagcgc tctggtggca gaacaagcgg caaaaactcc
     6361 ggatgctccg gcgctggcag atgcgcatta ccagttcagc tatcgggaaa tgcgtgagca
     6421 ggtggtggcg ctggcgaatc tgctgcgtga gcgcggcgtt aaaccgggcg acagcgtagc
     6481 ggtggcgcta ccgcgctcgg tctttttgac cctggcgcta catgcgattg ttgaagcagg
     6541 tgcggcctgg ctaccgctgg ataccggtta tccggacgat cgcctgaaaa tgatgctaga
     6601 agatgcgcgt ccgtcgctgt taatcaccac cgacgatcaa ctgccgcgct ttgccgatgt
     6661 tccagattta accaaccttt gctataacgc cccgcttaca ccgcagggca gtgcgccgct
     6721 gcaactttca caaccgcatc acacggctta tatcatcttt acctctggct ccaccggcag
     6781 gccgaaaggg gtaatggtcg ggcagacggc tatcgtcaac cgcctgcttt ggatgcaaaa
     6841 tcattatccg cttacaggcg aagatgtcgt tgcccaaaaa acgccgtgca gttttgatgt
     6901 ctcggtgtgg gagtttttct ggccgtttat cgcaggggca aaactggtga tggctgaacc
     6961 ggaagcgcac cgcgacccgc tcgctatgca gcaattcttt gccgaatatg gcgtaacgac
     7021 cacgcacttt gtgccgtcga tgctggcggc atttgttgcc tcgctgacgc cgcaaaccgc
     7081 tcgccagagt tgcgcgacgt tgaaacaggt tttctgtagt ggtgaggcct taccggctga
     7141 tttatgccgc gaatggcaac agttaactgg cgcgccgttg cataatctat atggcccgac
     7201 ggaagcggcg gtagatgtca gctggtatcc ggcttttggc gaggaactgg cacaggtgcg
     7261 cggcagcagt gtgccgattg gttatccggt gtggaacacg ggcctgcgta ttcttgatgc
     7321 gatgatgcat ccggtgccgc cgggtgtggc gggagatctc tatctcaccg gtattcaact
     7381 ggcgcagggg tatcttggac gacccgatct gaccgccagc cgctttattg ccgatccttt
     7441 tgctcctggt gaacggatgt accgtaccgg agacgttgcc cgctggctgg ataacggcgc
     7501 ggtggagtac ctcgggcgca gtgacgatca gctaaaaatt cgcgggcagc gtattgaact
     7561 gggcgaaatc gatcgcgtga tgcaggcgct gccggatgtc gaacaagccg ttacccacgc
     7621 ctgtgtgatt aaccaggcgg ctgccaccgg tggtgatgcg cgtcaattgg tgggctatct
     7681 ggtgtcgcaa tcgggcctgc cgttggatac cagcgcattg caggcgcagc ttcgtgaaac
     7741 attgccacca catatggtac cggtggttct gctgcaactt ccacagttac cacttagcgc
     7801 caacggcaag ctggatcgca aagccttacc gttgcctgaa ctgaaggcac aagcgccagg
     7861 gcgtgcgccg aaagcgggca gtgaaacgat tatcgccgcg gcattctcgt cgttgctggg
     7921 gtgtgacgtg caggatgccg atgctgattt cttcgcgctt ggcggtcatt cgctactggc
     7981 a
//
LOCUS       DAHBHC010000139         7945 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHBHC010000139 DAHBHC010000000
VERSION     DAHBHC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.142"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..242)
                     /locus_tag="M2A35_004698"
     CDS             complement(<1..242)
                     /locus_tag="M2A35_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCB7200895.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFEL"
     gene            complement(979..2427)
                     /gene="cusS"
                     /locus_tag="M2A35_004699"
     CDS             complement(979..2427)
                     /gene="cusS"
                     /locus_tag="M2A35_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCB7200896.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(2417..3100)
                     /gene="cusR"
                     /locus_tag="M2A35_004700"
     CDS             complement(2417..3100)
                     /gene="cusR"
                     /locus_tag="M2A35_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCB7200897.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3257..4639
                     /gene="cusC"
                     /locus_tag="M2A35_004701"
     CDS             3257..4639
                     /gene="cusC"
                     /locus_tag="M2A35_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCB7200898.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            4663..4995
                     /gene="cusF"
                     /locus_tag="M2A35_004702"
     CDS             4663..4995
                     /gene="cusF"
                     /locus_tag="M2A35_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCB7200899.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            5011..6234
                     /gene="cusB"
                     /locus_tag="M2A35_004703"
     CDS             5011..6234
                     /gene="cusB"
                     /locus_tag="M2A35_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCB7200900.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            6246..>7945
                     /gene="cusA"
                     /locus_tag="M2A35_004704"
     CDS             6246..>7945
                     /gene="cusA"
                     /locus_tag="M2A35_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCB7200901.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     "
BASE COUNT         2005 a         1960 c         2096 g         1884 t
ORIGIN      
        1 agttcaaacc aggtcaccac gcctttcacc cggcgctgta cgtcatcgcc ctgccatatc
       61 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagcgaa gagagtgact ggttcagatg aaaggaaacc
      181 accgcaaaag cgtccggcgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct ccagtaaaat gaataatgat ccgtaccgta caactgcgga taatgtgata
      301 agcgggacga cttcagacgt tattagggaa ggaacagaaa atgctatggt tatgtgagaa
      361 ttttgtttaa atattacttt acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa
      421 gctcaacctc ttgtttcttg gcgttattca ggacaggtac gcatatcaca acgagtaaca
      481 aaaacgcaaa gttaaaccaa tattcataat atatttttat tcacagcaat aaataatcca
      541 attaacatta ttaaaatgaa tgattgcata agaaatatat gctaccgctc caccctgata
      601 acatgatctg tacattgcac atcttaacgt tgttaaatac tggctcaata gtggatattt
      661 taaattagga atttatcaaa ttcacttgat aaatagattg agtgaataat ataacaatta
      721 atgatgatta atcgcgatta aattattcat tgctttatat gatagatggc ttgtttataa
      781 cgatgaaaat ttagaaaggg cattgtgatc tacaattgga agaaacacag atttaactaa
      841 gtttcatata caaacatctc gtgaaacttt gaaaaataaa agccttcgat atttcatatg
      901 tttaataagt ttttctctaa tttgttgatc aacaatacca ggaggtgatg caccacgccc
      961 tgaatttgaa cttaataatc actctctctc cggcagcata atcacaaacc ttgtcccccg
     1021 cgcatctgac gttactgcaa ccgtgccttt atgcgcgaca acaatcgatt tcactatcgc
     1081 cagcccaatg ccgctacctt cgccttttcg ctggcgggaa gggtcaacgc ggtagaaacg
     1141 gtcaaacaat cgcggtaagt gctcgggcgc aatgggcgta ccggggtttt cgacggtaac
     1201 ttgcacctgg tgatcgaccg tctggcagcg cactacaatt gcctctccgg gcggcgtata
     1261 acgcagggca ttagaaagca ggttgcttaa cgcccgacgc agcatcagcg gatcgcccgc
     1321 aacctgacat tcgtcgccaa caaatcgcaa ctccacgccg cgatcttccg ctaacgcctc
     1381 gaaaaaatcg aacactttgc cgacttcatc cgccaggttg agcattttct tttcggggat
     1441 tagctggttg ttatcggcct gagcgagaaa cagcatatcg ctgaccattt tcgccattcg
     1501 cgtcagctct tcgagattag agtagagcac atcttccagc tccttctggc tgcgagactg
     1561 gctgagggcg atttccgttt gcgtgatgag attggtaatc ggcgtgcgaa tttcgtgagc
     1621 gatatccgct gagaaattgg actggcgggt aaagacatcc tcaatacgct cgatcatatg
     1681 gttgaacgac agtaccagct gttccagctc aatgggcacg gtctgcgggt caaggcgaac
     1741 gtcgagatct ttcgaggtaa tattctggat ttgacggctg acgctgcgga tcggcgcgtg
     1801 acctttatgt accgccaata gcacgataaa gacgatcagg atgctgatga tcgatgcggt
     1861 cataataagt ttattcatca aatcatttat gtaatgaaga tgaaaatcga tcgaaagcgc
     1921 gatgtagagc gtataaatcg gtttgccgtc caccaacggg ccaaccgaca agttaatcat
     1981 ccgccagttg ctgtgttcca tatgcccgtg accatgccct ggcatcataa tcgtcgggcc
     2041 ggaaaggaga tacacctcgc cgccccgcgc gtctttatcg ggtatggcgt cacgcgtaaa
     2101 ctcgcggata tccggcgcac cgggggagtg atacaccgtt ttaccatggc tatctgccag
     2161 ggagatcaac acgttggaat aaccactgac gatatcttcc agcgtcatta agcgtcgggc
     2221 ttgcgtctcg tcaggatgat taagtacccg ttcaagcgtg gcgctaattt cttttaaatc
     2281 attaatatcc tgctcggcaa aatgcacttt tactgagtgg atcatgatcc atgcaaaggc
     2341 gaaaaacgct gcgatggtgg ccaggctgat aaaaaaggtc aggcgggttg ccagcgaaaa
     2401 cgggcgctga aatggcttac tgaccatccg gcacctcaag catgtaaccc acgccgcgca
     2461 cggtctgaat tagctttggc tcaaagtcgt tgtcgatttt gccgcgcagc cgcttcaccg
     2521 ctacatcaat ggcgttagtg tcgctgtcga aattcatgtc ccacacctgc gaggcgataa
     2581 gcgagcgggg cagcacttcg ccctgatggc ggaggaaaaa ctccagcaga gtaaactctt
     2641 tactggtcag agtgatgcgc gtgccgctgc gggtgacttt gcggctgacg agatcgacca
     2701 tcagatcggc aacctgaaac tgactttcga taatcaccgc cgccccgcgc cgcaggagag
     2761 tgcgcacccg cgccagcagt tcagcaaaag cgaacggctt caccagatag tcatccgccc
     2821 ccaactccag ccccttgacg cgatgttcaa tggtgccaag cgcggtaagc aacagaatcg
     2881 gcatcccttt attggcggaa cgcaacatgc gcacgatatc ccagccgttc acgtccggca
     2941 gcataatatc gaggattatc agatcataat caccggtcat cgccagatgg tagccattca
     3001 acccgttgtc ggccaaatcg accacaaaac cggcttcggt taaccctttg gtcaagtatt
     3061 ctccggtttt cttttcatct tcgacaatca acagtttcat atttcctccg catgttgccc
     3121 gggcaattct agagtagcgg gatcagatgg caatcgctta ttggcaaaat gacaattttg
     3181 tcatttttct gtcaccggaa aatcagagcc tggcgagtaa agttggcggc ataaaatcac
     3241 cagaaattat gagcctatgt ctccttgtaa acttctgcca ttttgtgtgg cccttgcgct
     3301 aaccggttgt tcactggcac cggattatca gcgtccggca atgcccgtgc cgcagcagtt
     3361 ctcactcagc cagaacggcc tggttaacgc agcagataac tatcagaacg cgggctggcg
     3421 cacctttttt gttgataatc aggtgaagac gctgattagc gaggcgctgg tgaataatcg
     3481 ggatttgcgc atggcgacgc tgaaagtgca ggaggcacgg gcgcaatatc gtctgaccga
     3541 tgccgaccgc tacccacagc tcaatggcga gggcagcggc agctggagcg gtaatcttaa
     3601 agggaatacc gccacgacac gggagttttc gaccggcctt aacgccagct ttgacctcga
     3661 ttttttcggt cgcttaaaga acatgagcga agccgagcga caaaattatt tagccactga
     3721 ggaagctcag cgcgcggtgc atattctgct ggtttctaat gtcgcgcaaa gctatttcaa
     3781 tcagcaactg gcgtatgcgc aattgcaaat agccgaagaa acgctgcgta attatcagca
     3841 gtcatatgcg tttgtcgaaa aacaactgtt gacaggtagc agtaatgttc tggcgctgga
     3901 acaggctcgc ggggtgatag aaagtacccg cagcgacatc gctaaacgtc agggggaact
     3961 ggcgcaggcg aataatgcat tgcaactgtt attgggaagc tacggcaagc tgccgcaagc
     4021 gcagacagta aacagcgaca gcctgcaaag cgttaaatta ccggcgggat tgtcgtcgca
     4081 aatcttattg cagcgcccgg atattatgga agctgaacac gcgttaatgg cggctaatgc
     4141 caatattggc gctgcgcgtg cggcattttt cccgtctatc agcctgacca gcggaatatc
     4201 aaccgccagc agcgatctgt cttcattatt taacgctagc agcgggatgt ggaattttat
     4261 tcctaaaatt gagatcccca tttttaatgc cggacgcaac caagccaatc tggatatcgc
     4321 cgaaattcgc cagcagcagt cggtagtgaa ttatgaacag aaaatccaga acgcctttaa
     4381 agaagtggca gatgcgctgg cattacgtca aagcctgaac gatcaaatca gcgcccagca
     4441 gcgttatctg gcgtcgctgc aaattacttt gcaacgggcg cgggcattat atcagcacgg
     4501 cgcggtaagt tatctggaag tgctggatgc cgagcgttct ttatttgcaa cccgacaaac
     4561 tttactcgac ctgaattatg cccgtcaggt taacgaaatt tctttgtata ccgccttagg
     4621 gggcggttgg cagcaataat ttttaactcc aggagagaat aaatgaaaaa agcactgcaa
     4681 gtcgcaatgt tcagtctgtt taccgttatt ggctttaatg cccaggctaa cgaacatcat
     4741 catgaaacca tgagcgaagt acaaccgcag gttattagcg ccactggcgt ggtaaagggt
     4801 attgatctgg aaagcaaaaa aatcaccatc catcacgatc cgattgctgc cgtgaactgg
     4861 ccggagatga ccatgcgctt taccatcact ccgcagacga aaatgagtga aattaaaact
     4921 ggcgacaaag tggcgtttaa ttttgtccag cagggcaacc tttctttatt acaggatatt
     4981 aaagtcagcc agtaacccag gtttaatgag atgaaaaaaa tcgcgcttat tatcggcagc
     5041 atgatcgcgg gcggtattat ttctgcggca ggttttacct ggtttgcaaa ggctgaacca
     5101 cccgcagaaa aaacgtcgac cgcagaacgt aaaatcttat tctggtacga cccgatgtac
     5161 cccaatacgc ggttcgataa accaggtaaa tcgccgttta tggatatgga tctggtgccg
     5221 aaatatgccg atgaagagag ttctgcgtct ggtgtgcgca ttgacccgac tcagacgcag
     5281 aatctggggg tgaaaacggc taccgtcacg cgcgggccgc tgacttttgc ccagagtttc
     5341 ccggcgaatg tcagttacaa cgagtatcag tatgccattg tgcaggctcg cgctgccggg
     5401 tttatcgaca aggtgtatcc gcttaccgtg ggcgataaag tacaaaaggg cacaccgctt
     5461 ctcgacctga ccattcctga ctgggtggaa gcgcagagtg agtatttact gctgcgcgaa
     5521 accggcggta cggcgaccca gactgaaggc attcttgagc ggctgcgact ggcgggaatg
     5581 ccggaggcgg atattcgccg cctgatcgcc acgcaaaaaa tccagactcg ctttacgctc
     5641 aaagcgccca ttgatggcgt gatcaccgcg tttgatctgc gcgcgggaat gaatatcgcc
     5701 aaagataacg tggtagcgaa aattcagggt atggacccgg tgtgggtcac tgctgcgatc
     5761 ccggagtcta tcgcctggct ggtgaaagat gcctcgcagt ttaccctcac cgttccggcg
     5821 cgaccggata aaacactcac catccgcaaa tggacgctgc tacctggcgt ggatgccgcg
     5881 acccgcacgc tgcagctgcg tctggaagtc gacaacgccg acgaggcgct aaaaccgggc
     5941 atgaacgcct ggttgcaact caacaccgcc agcgaaccga tgctgctcat tccgtcacaa
     6001 gcgctgattg ataccggcag cgaacagcgg gtgattaccg ttgatgccga cgggcgcttt
     6061 gtaccgaaac gcgttgctgt tttccaggca tcgcaaggcg tcaccgcatt acgctctggt
     6121 ctggcggaag gtgaaaaggt ggtttccagc ggcctgttcc tgattgattc cgaagccaat
     6181 atttctggcg cactggagcg gatgcgctct gaaagtgcta cccatgcgca ttgagggaat
     6241 aaccaatgat tgaatggatt attcgtcgct cggtggcgaa ccgttttttg gtgctgatgg
     6301 gcgcgttgtt tctgagcatc tggggcacct ggaccatcat taatacgcca gtggatgcgc
     6361 tgccggatct ctccgatgtg caggttatta ttaaaaccag ctatcccggt caggcaccac
     6421 aaatcgttga aaatcaggtg acttatccgc taaccaccac catgttgtcg gtgcctggcg
     6481 cgaagactgt gcgcggtttc tcacagtttg gcgactctta tgtgtatgtc attttcgaag
     6541 atggcaccga tccgtactgg gcgcgttcgc gggtgctgga gtacctcaac caggtacagg
     6601 gtaagctgcc tgcgggagtc agcgccgagc tggggccaga tgccacgggt gttggctgga
     6661 tctatgaata tgcactggtg gatcgcagcg gtaagcacga tctggccgat ttacgctcat
     6721 tacaggactg gtttctcaaa tatgagctga aaaccatccc tgacgttgcg gaagtggcgt
     6781 cggtgggcgg tgtggtgaaa gagtatcagg tggttatcga tccccagcgc ctggcgcagt
     6841 atggcatcag tctcgccgaa gtaaaaagcg cgctggatgc ttcaaaccag gaagcgggcg
     6901 gttcgtcgat cgaactggcg gaagcggaat atatggtgcg cgccagcggc tatctgcaaa
     6961 cgctcgacga ctttaatcac atcgttttaa aagccagtga aaatggcgtg cccgtttatc
     7021 tgcgcgatgt tgcgaaggtc cagattggcc cggagatgcg ccggggcatt gccgaactaa
     7081 acggcgaagg cgaagtggcg ggcggggtgg tgatcctgcg atccggcaaa aacgcccgag
     7141 aagtgatcgc cgccgtgaag gacaaactgg aaacgctgaa aagtagtctg ccggaaggcg
     7201 tggagatagt tacaacatac gatcgcagcc agctcattga ccgcgccatc gacaacctca
     7261 gcggcaagtt gctggaagag tttattgtgg tggcggtagt ctgtgcgctg tttctctggc
     7321 atgtgcgctc ggcgctggtg gcgattattt cgttgccgct ggggttgtgt attgctttta
     7381 ttgtcatgca cttccaggga ctgaatgcca atattatgtc gttgggtggc attgcgattg
     7441 ccgtcggggc gatggtcgat gccgctatcg tcatgatcga aaatgcacat aaacggctgg
     7501 aagagtggca gcaccagcat cctgacgcca cgctggataa taaaacgcgc tggcaggtga
     7561 tcaccgatgc gtctgttgaa gtggggccgg cgctatttat cagtctgctg attatcacgt
     7621 tgtcgtttat cccgatcttc accctggaag ggcaggaagg gcgtctgttt ggcccgttgg
     7681 cgttcaccaa aacgtatgcg atggcgggtg cggcgctgct ggcgatcgta gtgatcccga
     7741 tcctgatggg ctactggatc cgtggcaaaa ttccgccgga aagcagtaac ccgctcaatc
     7801 gctttttgat tcgtgtttat catccgctgt tgctgaaagt actgcactgg ccgaaaacca
     7861 cgctgctggt ggcggcgctt tcggtgctga cggttctctg gccgctcaat aaagttggcg
     7921 gggaattttt accgcagatc aatga
//
LOCUS       DAHBHC010000140         7907 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHBHC010000140 DAHBHC010000000
VERSION     DAHBHC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.143"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="M2A35_004705"
     CDS             complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="M2A35_004705"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCB7200902.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1400..1813
                     /gene="holD"
                     /locus_tag="M2A35_004706"
     CDS             1400..1813
                     /gene="holD"
                     /locus_tag="M2A35_004706"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCB7200903.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1782..2228
                     /gene="rimI"
                     /locus_tag="M2A35_004707"
     CDS             1782..2228
                     /gene="rimI"
                     /locus_tag="M2A35_004707"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCB7200904.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2243..2920
                     /gene="yjjG"
                     /locus_tag="M2A35_004708"
     CDS             2243..2920
                     /gene="yjjG"
                     /locus_tag="M2A35_004708"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCB7200905.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3011..4600
                     /gene="prfC"
                     /locus_tag="M2A35_004709"
     CDS             3011..4600
                     /gene="prfC"
                     /locus_tag="M2A35_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCB7200906.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4993..5598
                     /gene="osmY"
                     /locus_tag="M2A35_004710"
     CDS             4993..5598
                     /gene="osmY"
                     /locus_tag="M2A35_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCB7200907.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSVYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5725..5886
                     /locus_tag="M2A35_004711"
     CDS             5725..5886
                     /locus_tag="M2A35_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCB7200908.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6008..7081
                     /locus_tag="M2A35_004712"
     CDS             6008..7081
                     /locus_tag="M2A35_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCB7200909.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7078..7860
                     /locus_tag="M2A35_004713"
     CDS             7078..7860
                     /locus_tag="M2A35_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCB7200910.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
BASE COUNT         1892 a         2033 c         2143 g         1839 t
ORIGIN      
        1 accatcaatt cttaaaaaga attgctacca cggaggcgca ttctagtggt tttcagcttt
       61 tcgtcaatag ttaattatcg ccggaggtgt aattgctgga aaaatgtcca tcaggaaact
      121 agcgtgcagg tttggtaggc atgcgggggc agatgccaga tgcgacgctg gcgcgtctta
      181 tctggcctac gaagggctaa cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca
      241 tccgacacgg tattcggcga gataattaac ctttcttcgc ctggcgggtc ataatggcgc
      301 gatacacctt gaaacgcccg gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca
      361 gcacgtccgg gtaaggcagg aaggcgttcg ctacaattcg cagctcgccg ccgctattaa
      421 gatgacgcac cgcgccgcga atcagcgttt gcgccgcatc caggctggtt tgcatcccat
      481 cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg gaaaagacgt
      541 tgctggcaaa gacttcacct tcaacaccgt tggccgcaag tgttgcgcgg ctggcttcta
      601 ccgctggcgc agagacatcg cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg
      661 caactgaaag cacacccgcg ccacagccga catccagcac tttacctttc gtgtgcgggg
      721 ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta aacacgccag
      781 gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa
      841 ataccggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt
      901 tattcaacgg cgcataatct gccagcatct gctcggcgct gcgcacgccg ctgcggttct
      961 cgccaacgac aaaaatatct gtccccactg gcagcagaga aagtaaattc atcaactgga
     1021 actgggcttc cggtttgttc ttcggccagt agtaaatcag cgtatcgcaa tctgcgacgt
     1081 catccaccgt ggcgaccaga ctgaaacggg cgttatcccc catctggcgg cttaatacct
     1141 gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca
     1201 ggtcatcctg taagtctccg gcaaacagaa tacggctttg ttcgaaatca tcactgtgac
     1261 gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca attgatactg
     1321 gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg
     1381 acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc aactgggcat
     1441 tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca ttgcgatccc
     1501 ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgaccg atcctttagt
     1561 gagcgatgtt ctgcgcgcat taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga
     1621 aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg gtactgacga
     1681 accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa
     1741 cccaacggca cgcgccgcgt tatggcaaca gatttgcaca tatgaacacg atttcttccc
     1801 tcgaaacgac tgatttacca gcggcttacc acattgaaca acgcgcccac gcctttccgt
     1861 ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt cagttaacgc
     1921 aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa gctacattgt
     1981 tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggtg ctgctggaac
     2041 atctgatcga cgaactggaa aaacgcggcg tggcgacact atggctggaa gtccgtgctt
     2101 caaacgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc
     2161 gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg ttgccaatca
     2221 gtatgtaaca caaggtggaa taatgaagtg ggactggatt ttctttgatg ccgatgaaac
     2281 gctgtttacc tttgactcat tcaccggcct gcagcggatg tttcttgatt acagcgtcac
     2341 ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt gggtggatta
     2401 tcaaaacggc gcgatcactt cattacagct tcagcacggg cggtttgaga gctgggccga
     2461 acggctgaaa gttgaagcag gcttgcttaa cgatgccttt attaatgcga tggcggaaat
     2521 ctgtacgccg ttgccgggcg cggtttctct gcttaacgcc attcgtggca acgctaaaat
     2581 cggcatcatc accaacggtt ttagcgcctt gcagcaagtg cgtctggaac ggacgggcct
     2641 gcgtgattac ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcta aaccgaataa
     2701 gaaaattttc gattatgcgc tggaacaggc gggcaatcct gaccgttcac gcgtgctgat
     2761 ggttggcgac actgccgagt ccgatattct cggtggcatc aacgccgggc ttgcgacctg
     2821 ctggctgaat gcacaccatc gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc
     2881 ttcgttgcat gaactggagc agctcctgtg taaacactga ttgcctcccc cccgttgatg
     2941 ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga
     3001 agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc
     3061 cattatttct cacccggacg ccggtaagac taccatcacc gagaaggtgc tgctgttcgg
     3121 acaggccatt cagaccgccg gtacagtaaa aggccgtggt tccaaccagc acgctaagtc
     3181 ggactggatg gagatggaaa agcagcgtgg gatctccatt actacgtctg tgatgcagtt
     3241 tccgtatcac gattgcctgg ttaacctgct cgacaccccg gggcatgaag acttctcgga
     3301 agatacctat cgtaccctga cggcggtgga ctgctgcctg atggttatcg acgccgcaaa
     3361 aggtgttgaa gatcgtaccc gtaagctgat ggaagttacc cgtctgcgcg acacgccgat
     3421 cctcaccttt atgaacaaac ttgaccgtga tatccgcgac ccgatggaat tgctcgatga
     3481 agttgagaac gagctgaaaa ttggctgcgc accgatcacc tggccaattg gctgcggcaa
     3541 gctgtttaaa ggcgtttacc acctttataa agatgaaacc tatctctatc agagcggtaa
     3601 agggcacacc attcaggaag tccgcattgt taaagggctg aataacccgg atctcgatgc
     3661 tgcggttggt gaagatctgg cacagcagct gcgtgacgaa ctggaactgg ttaaaggcgc
     3721 gtctaacgag ttcgacaaag agctgttcct tgcgggcgaa atcactccgg tattcttcgg
     3781 tactgcgctg ggtaacttcg gcgtcgatca tatgttggat ggcctggtgg agtgggcacc
     3841 tgcgccgatg ccgcgtcaga ctgatacccg taccgtacaa gcgagcgaag acaaatttac
     3901 cggcttcgta tttaaaattc aggccaacat ggacccgaaa caccgcgacc gcgtggcgtt
     3961 tatgcgcgta gtatccggta aatatgaaaa aggcatgaag ctgcgccagg tgcgtactgc
     4021 gaaagatgtg gttatctccg acgcgctgac ctttatggcg ggcgaccgtt cgcacgttga
     4081 agaagcgtat ccgggcgata tcctcggcct gcacaaccac ggcactattc agatcggcga
     4141 cacctttacc cagggtgaga tgatgaagtt caccggtatt ccgaacttcg caccagaact
     4201 gttccgtcgt atccgcctga aagatccgct gaagcaaaaa cagctgctca aagggctggt
     4261 acagctttcc gaagagggcg cggtgcaggt gttccgtccg atctccaaca acgatttgat
     4321 cgttggtgct gttggtgtgc tgcagtttga tgtggtggta tcgcgcctga agagcgaata
     4381 caacgttgaa gcagtatatg aatcagtcaa cgttgccact gcccgctggg tagaatgtgc
     4441 ggacgcgaag aaattcgaag agttcaagcg taagaacgaa agccaactgg cgcttgatgg
     4501 cggcgataac ctcgcttaca tcgctaccag catggtcaac ctgcgcctgg cgcaggaacg
     4561 ttatcccgac gttcagttcc accagacccg cgagcattaa ttcttgcctc cagggcgcgg
     4621 tagccgctgc gccctgtcaa tttcccttcc ttattagccg cttacggaat gttcttaaaa
     4681 cattcacttt tgcttatgtt ttcgctgata tcccgagcgg tttcaaaatt gtgatctata
     4741 tttaacaaag taatgacatt tctgacggcg ttaaataccg ttcaatgcgt agatatcagt
     4801 atctaaagcc gtcgattgtc attctaccga tattaataac tgattcagag gctgtaatgg
     4861 tcgttattca tcacccatcg cttttgtgat ggcgaccatt gacttctgta gagggtgaag
     4921 tctctcccta ttcagcaatg caacctcgtg ttgccaggct caaattacga gcaaacatac
     4981 aggaataaat cgatgactat gacaagactg aagatttcga aaactctgct ggctgtaatg
     5041 ttgacctctg ccgtcgcgac cggctctgtc tacgcggaaa acaacgcaca gactaccaat
     5101 gaaagcgcag ggcaaaaagt cgatagctct atgaataaag tcggtaattt catggatgac
     5161 agcgccatca ccgcgaaagt gaaggcggcg ctggtggatc atgacaacat caagagcacc
     5221 gatatctctg tcaaaaccga tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag
     5281 gctcaggccg aagaggcagt gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc
     5341 gacaaactgc acgttcgcga cgctaaagaa ggctctgtga agggctacgc gggtgacacc
     5401 gccaccacca gtgaaatcaa agccaaactg ctggcggacg atatcgtccc ttcccgtcat
     5461 gtgaaagttg aaaccaccga cggcgtggtt cagctctccg gtaccgtcga ttcccaggca
     5521 cagagcgacc gtgctgaaag tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat
     5581 gatctgaaaa ctaagtaatt cgtcgtaatt cgtcctcccg aactttgtcg ggaggcgcga
     5641 tgtgcaccac actaaaaata tcgcattgag tgattttcac gctcatatca agcggtgaca
     5701 ttaactatgg taaaggagac gcttatgttt cgttggggca tcatatttct ggttatcgcg
     5761 ttaatcgccg ccgcacttgg gtttggtggt ctggccggta ccgctgcagg cgcagctaaa
     5821 attgtctttg tcgtcgggat tattctgttc ctggtgagtt tgttcatggg ccgaaaacga
     5881 ccctagattt cagaccatta agaaaatttt atccaaagcc agtccagcgg actggctttt
     5941 gcggttttag cgaataataa attgcgttac tttgtcattc tttcacaaca aaaacaggaa
     6001 ggcagaggtg gggcagcgaa tacctgtaac gcttggtaat attgcgccgt tgtcgctaag
     6061 gccgttccag cctggacgaa tagctctggt gtgcgaaggc ggcggacagc gtggaatttt
     6121 cacggctggc gtgctggatg agtttatgcg cgcgcagttt aatcctttcg atctttatct
     6181 cggcacatct gccggggcgc agaacctctc ggcgtttatc tgcaatcagc ccggttacgc
     6241 gcgcaaagtc atcatgcgct ataccacaaa acgcgaattt ttcgatccat tgcgctttgt
     6301 ccgtggagga aatcttatcg atctcgactg gctggtggag gccaccgcaa gccagatgcc
     6361 gttgcaaatg gataccgccg cgcggttgtt tgacagcggc aaatcgtttt atatgtgcgc
     6421 ctgtcgtcag gatgactacg cgcctaatta ctttttacca accaaacaaa actggctgga
     6481 tgtgattcgc gcctccagtg cgatacctgg cttttatcgt agcggagtgt cgctggaagg
     6541 cattaactac ctggatggcg ggatcagtga tgcgattccg gttaaagagg cggcaaggca
     6601 gggcgctaaa acgttggtcg tcattcgcac tgtgccgtca caaatgtact acacgccgca
     6661 gtggttcaaa cgcatggaac gctggctggg tgacagtagc ctgcagccgc tggtcaatct
     6721 ggtgcagcat catgaaacca gctatcgtga cattcagcaa tttattgaga aaccaccggg
     6781 caagctgcgg atattcgaaa tttatccgcc gaagccatta catagtatcg cgcttggcag
     6841 tcggattccg gcgctgcgtg aagactataa acttgggcgt ttatgcggtc gttatttcct
     6901 cgccacggtt ggcaagctat taactgaaaa agcgccgctt acccgccatc tggtgccagt
     6961 ggtgacgccg gaatcgatcg ttattccgcc tgcgccagtc gccaacgata cgctggttgc
     7021 cgaagtgatt gacgctccgc aggcgaacga cccgacattt aacaatgagg atctggcttg
     7081 atttgccgtt ttatcgacac ccactgccat tttgatttcc cgccgtttag tggcgatgaa
     7141 gaggccagcc tgcaacgcgc ggcacaagcg ggcgtaggca agatcattgt tccggcgaca
     7201 gaggcggaaa attttgcccg tgtgcaggcg ttagcggaaa aatatcaacc gctgtatgcc
     7261 gcattgggct tgcatccagg tatgttggaa aaacatagcg atgtgtctct tgagcagcta
     7321 cagcaggcgc tggaaaggcg tccggcgaag gtggtggcag tgggggaggt cggtctggat
     7381 ctcttcggcg acgatccgca acttgagagg cagcagtggt tactcgacga acagctgaaa
     7441 ctggcgaaac gctacgatct gccggtgatc ctgcattcac ggcgcacaca cgacaaactg
     7501 gcgatgcatc ttaaacgcca cgatttatcg cgcactggcg tggttcacgg tttttccggc
     7561 agcctgcaac aggccgagcg gtttgtacag ctgggctaca aaatcggcgt aggcggtact
     7621 atcacctatc cacgcgccag taaaacccgc gatgtcatcg caaaattacc gctggcatcg
     7681 ttgttgctgg aaaccgacgc gccggatatg ccgctcaacg gctttcaggg gaagcctaac
     7741 cgcccggaac aggctgtgcg ggtgtttgac gtgctttgcg agttacgtcc ggaaccagaa
     7801 gatgagattg ccgaagtgtt gcttaataat acgtatgcgg tgtttagcgt tcgtgggtag
     7861 tttacccttc gtaggcagca tccggcagtc ggcgcataat gcctgat
//
LOCUS       DAHBHC010000141         7899 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHBHC010000141 DAHBHC010000000
VERSION     DAHBHC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.144"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            297..1568
                     /locus_tag="M2A35_004714"
     CDS             297..1568
                     /locus_tag="M2A35_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCB7200911.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1598..2602)
                     /gene="dppF"
                     /locus_tag="M2A35_004715"
     CDS             complement(1598..2602)
                     /gene="dppF"
                     /locus_tag="M2A35_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCB7200912.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGISFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2599..3582)
                     /gene="dppD"
                     /locus_tag="M2A35_004716"
     CDS             complement(2599..3582)
                     /gene="dppD"
                     /locus_tag="M2A35_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCB7200913.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3593..4495)
                     /gene="dppC"
                     /locus_tag="M2A35_004717"
     CDS             complement(3593..4495)
                     /gene="dppC"
                     /locus_tag="M2A35_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCB7200914.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4505..5524)
                     /gene="dppB"
                     /locus_tag="M2A35_004718"
     CDS             complement(4505..5524)
                     /gene="dppB"
                     /locus_tag="M2A35_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCB7200915.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5675..7282)
                     /gene="dppA"
                     /locus_tag="M2A35_004719"
     CDS             complement(5675..7282)
                     /gene="dppA"
                     /locus_tag="M2A35_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCB7200916.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         1824 a         2126 c         2009 g         1940 t
ORIGIN      
        1 cggcttgccg ctgattttac gattgctttt tactgtcagc acccggcaat cttcctcatt
       61 gcgcgatgcc ttccagctta atttctttat ttgcaatagt tgcagttcat atccgggctg
      121 actccagact ggtagtgaaa tgcgcaattt tgtagaattg cgcctcctcg cgacctcccc
      181 agtgaatctg ttgcctcatt ttttacatac tgcgtattcg acttctccac ctgttgcgca
      241 agagaaactg ggtttattca tttttgcgag gccgacttct ttctggacag gactttatgc
      301 agcacaacac actatcgaaa cacaatcaga aattgccgtt tacacgctac gacttcggct
      361 gggttttatt atgcataggc atggcgattg gtgccggaac cgtgctgatg ccagtacaaa
      421 ttggcttgaa gggaatttgg gtatttatta ccgcagcgat cattgcttat cctgccacct
      481 gggtagtgca ggacatttat ttaaaaaccc tttctgaaag cgattcctgt aatgactaca
      541 ccgatattat cagtcattac ctggggaaga actggggaat tttcctcggg gttatctact
      601 ttttgatgat tatccacggg atttttatct actctctctc cgtggttttc gacagcgcct
      661 cgtacctgaa aaccttcggt ttaaccgatg ccgatctttc acaatctcta ctttataaag
      721 tcgctatttt cgccgtactg gtggcgattg cgtctggtgg tgaacgatta ctgtttaaga
      781 tttccgggcc aatggtggtg gtcaaagtag ggattattgt cgtgttcggt tttgcgatga
      841 tcccgcactg gaatttcgcc aatataaccg ccttcccgca agcctccgtc tttttccgcg
      901 atgtcttgct taccattcca ttttgcttct tttctgcagt atttattcag gtacttaacc
      961 caatgaatat tgcctatcgt aaacgggaag cggataaagt actggcaacc cggctcgcgc
     1021 tgcgtaccca ccgaattagt tatatcacgc tcatcgcggt gatcctgttt tttgcctttt
     1081 cgtttacctt ctcaattagc cacgaagaag ccgtttctgc ctttgaacaa aatatctcgg
     1141 cactggcgct ggccgcgcag gtgatccctg gacatatcat tcatatcacc tctacggtgc
     1201 tgaatatctt tgccgtactg accgcattct ttggcattta tctcggtttc cacgaggcta
     1261 ttaaaggcat tattctcaat ctgttaagcc gaattattga taccaagaaa attaactcac
     1321 gcatgctgac tctggcgatc tgcgctttta tcgtcattac attgacgatt tgggtttcgt
     1381 ttcgtgtatc ggtgctggtg ttctttcagt tgggaagccc gttatatggc attgtgtcgt
     1441 gcctcattcc gtttttcctg atctataaag tcgcacaact ggaaaaactt cgcggattta
     1501 aagcctggct aattctgctt tacggcattt tgctatgctt gtcgccactg ttgaagctga
     1561 ttgagtaaac cggagcgcat ggccccggtt ttgtgagtta acgctgcgga ttttcatcct
     1621 gatcaacagc aaaacaagct accagttgac cgccgtagtc ttttagctgc ggctgcaact
     1681 gggtgcaggg gccgaagcgc cgacgacagc gggcgttgaa ggcgcaaccc ggcggtggat
     1741 tcagcgggct tggcagttca ccggtgagtt tgatgcgttc gcggcgatcg tccgggttca
     1801 ggcgcggcgt cgcggagagt agcgcctgag tgtacggatg gcgcgggtta ttgaagattt
     1861 ggtctttcgt tcccttctcc acgcagcggc ccaggtacat caccatcact tcatcggcaa
     1921 tgtgctccac caccgacagg tcgtgggaga taaagacata agaaagcccc agttcctgct
     1981 gtaaatccat catcagattc agcacttgcg cacgcactga gacgtccagc gcagaaaccg
     2041 gctcatcggc aatcaccaca tccgggtcga gcatcagacc acgggcaata gcgatacgct
     2101 gacgttgtcc gccggagaac atatgcggat agcggtcata gtgctcggtt ttcaggccga
     2161 ctttcgccat catcgacagg gctttttccc gacgctgttc tttgcttaag ctggtgttaa
     2221 taagcagcgg ctcttcgaga atttgcccaa cttttttacg cggattcagc gaaccgtaag
     2281 ggttctggaa gacgatctgg attttctgcc gacgcagctt ctgcgcctgc ggatcgtgct
     2341 taagcagatc ctgcccctgg taatacagct cgccaccggt gggcatttca atcatcgtca
     2401 gcaaccgacc gagggtcgat ttaccgcagc cagattcgcc cactactgcc agcgttttgc
     2461 cacgttcaag gttaaacgaa atgccatcca gcgctttaac cagacgttcc ggcgcgaaca
     2521 tgcctttctt caccggataa tgttttttca ggtcgatagc ctgcaacagc ggttgttgcg
     2581 aggtggcctc ttgcgtactc atagtgtcgg cctcccggca tcatcaagtg ggtaatggca
     2641 tttggactga cgcccatcag cgagcatatt cagcgccggt tcttcagcgc gacatctgtc
     2701 agtggcatag gggcagcgcg ggttaagcag gcagccgttc gggcggtcgt acttaccggg
     2761 aacgacacct ggcaacgacg ccagacgttc tttgtcctga gcaaattctg gcagcgcacg
     2821 cagcaatgcc tgagtatacg ggtgacgcgg cgcatggaag atggcgtgcg catcaccggt
     2881 ttccaccacc tggcctgcat acatcacgat gattttatgt gccgcttccg ccaccagcgc
     2941 caggtcatgg gtaattaaca ccagcgccat gttctctttc tgctgtagct ccagcagtag
     3001 ttcgatgatt tgcgcctgaa tggtcacgtc cagcgcggtg gtcggttcat cggcaatcag
     3061 cagttttggc cgacaggcaa tcgccatggc gatcatcacg cgctggctca tgccgccgga
     3121 aagctgatgc gggtaaacat ccagacgcga tgccggatcg ggaataccga cctgattcag
     3181 caggtcaatc gctcgctgac ggcgggtact tttgttgccg ccctgatgca ccttaatcgc
     3241 ttccataatc tggaaaccca cggtgtagca cgggttaagg ctggtcatcg ggtcctggaa
     3301 gatcatcgcc acttcggcac ccaccaggtt gcggcgctct ttttctgaga tacgctgcaa
     3361 atcctggccg ttaaactcca gtttttctgc cattacgcgg cccggataat caatcagccc
     3421 cataatcgcc agtgaactga ccgacttacc ggagccggac tcacccacaa tcccgaccac
     3481 ttcgccctgt tttacgctgt agctgatgcg gtctacggcg cggaacggcg cgctttcgtc
     3541 gccgaaatgc accgataatt tatctacatt taataacgcc atctcgaacc tcttactgct
     3601 tcagtttggg atcgagcgcg tcacgcagac cgtcacccat caggttaaat gccagcaccg
     3661 tcagcaggat cgccagaccc gggaaggtca cgacccacca ggcgctttgc gcgaactgca
     3721 acacgtcgga gagcatggtg ccccactcag gcgttggcgg ctgtgccccc atgccgagga
     3781 aaccaagagc agccatatcg agaatggcgt tagagaaacc gagcgacgcc tgaacaatca
     3841 gcggcgcaag gcagttcggg aagatgttaa taaacatctg acgcatcgcc ccggcacccg
     3901 ccacgcgaga cgcggtgacg taatcgcggt taacttccac cagcacggcg gcgcgggtta
     3961 agcgcacata gtgcggcaag gcaacgaagg tcagtgccag cgcggcgtta ccaatcgacg
     4021 ggccgaaaat tgccaccagc accagcgcca gcagcagact tggcagcgcc agcatgatat
     4081 cgaccacgcg cataatgatg ttatcgacca ggccgccaaa gtaaccggcg atcaggccga
     4141 gaataacgcc cataatcagc gataacacaa ctaccagaca gccaaccagc agcgacaggc
     4201 gcgcaccgta catcaggcgc gacagcacat cacggcctac gtcatcggtg cccagcaagt
     4261 gcgccatgct gccgccttcc tgccaggctg gcggggcgag cagtgcatcg cggaactgtt
     4321 ccgccgggtt atagggtgca atccagttgg caaagatcgc gatgaacagc acgatgacga
     4381 cgtaaaccag cccgacgacc gcgcctttgt tgcgtttaaa atagtgccag aactcctgta
     4441 acggggtcat cggcaccggt gcgctaatca ctttattttc agtaacctgt gacatgatgt
     4501 ccccttactt cttatgacga atacgcgggt tcaccacgcc gtacagcaga tcgaccagca
     4561 agttgacgag gataatcatc gtcgccacca gcaatacgcc gccctgcact accggatagt
     4621 cgcggcgttg cagtgcgtca atcaaccagc gtcccagacc gggccacgag aagatggttt
     4681 cggtcagaat cgcccccgcc agcaatgttc ccacctgcag gccgataacg gtcaccaccg
     4741 gcagcatcgc gttacgcagc gcatggacga taatcacccg catgcgggtt agccctttgg
     4801 cgcgcgcggt gcggatgtaa tcctcgccca gcacttccag catcgaggag cgtgtcatac
     4861 gcacaatgac cgccagcgga atagtaccca gcacaatggc aggcaagatc atatgggcga
     4921 cggcatcgat aaagttgccg tcttcacccc agatggcggt gtcgattagc ataaaaccgg
     4981 ttaacggatt ggagtcatcg aggaacacca tatcgctcac gcgaccggag acgggcgtca
     5041 ggttccagtg caccgaaacc agcatgatca gcatcatgcc ccaccagaag atcggcatcg
     5101 agtagccggt cagcgccagg ccaaccgctg tgtgatcgaa aatggaaccg cgtttaaccg
     5161 cagccagcac accaaccgga ataccgactg ccgtagcaaa aatcatcgcg cagacgccaa
     5221 gttccagcgt ggcctgaaag cgcggcacga actcttccca tactggaatt cggcttttca
     5281 ttgaaatgcc tagatcgccg tgcattacgc cccaaatgta atggagatac tgctgccaca
     5341 tcggtttatc taagccgagt tcagccagca gctgcgcgtg acgctcaggg gagatcccac
     5401 gttcgcctgc catgatcatc accggatcgc ccgggatcat gtggacaaag gcaaatgtga
     5461 gaagggtaat accgataaac gtggggatga cgagtcccaa acgtcggaga ataaactgca
     5521 acataacccg gattctctat agtgacccgc agcctggaac tgcgtgtctg tattgctcac
     5581 aaatcaattc ccgcccttgt cggacagaga atgaagtatt gctgccggat ggcgctgcac
     5641 gcagaggcac agcgccaccg ggtatggctt ttaattattc gatagagacg ttttcgaagt
     5701 gatgtttgcc taatggatca accacatagc ctttaacttc tttgcgtacc ggttcaaaca
     5761 cggtggagtg agcgatgatc agtgccggag cctgatcgtg catcaccacc tgcgcttgct
     5821 tgtacagttc aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt
     5881 caaacggttt gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga
     5941 acagggtggc gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg
     6001 tctgatgttc gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt
     6061 tggcctgcac gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt
     6121 tcgggttata cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca
     6181 gacccgcttc tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt
     6241 cgtcgttata gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct
     6301 gataaaccgc tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt
     6361 tcacgtcatc cagcggtttt ttctgcacgt tatacgagag gtagccgacg ttcagccccg
     6421 gcatttccat caggttgatg gatttatcct gcttcatgcg agcgatatct gccgggttcg
     6481 ggtacggcat cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag
     6541 gggtaataga gaagaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg
     6601 ctttgtagcg gatacgggaa tctttttggt actgctgtaa ctggaatgga ccggttccga
     6661 ttgggttgag gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt
     6721 ttgacagaat agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag
     6781 tcagcacgaa ctgaactgtg ttgtcgtcca cctttttcac ttcgctgatc agctccggca
     6841 agcccatgcc ttcgaagtat tcgtagctac cgccagaaac tttatggtac gggttttgcg
     6901 cgtttttctg acgatcgaac gagaacacca cgtcatcggc gttcagttcg cgcgtcggtt
     6961 tgaattcttt attgtcatgc cacttcacac ctttacgcag atggaaggta taggttttac
     7021 cgtcttcgct gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt
     7081 taaattcaac caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg
     7141 taaacagctg cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag
     7201 cctgaacact tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg
     7261 actttttcaa ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct
     7321 ttgcagtggg ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat
     7381 gaggtgggga tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc
     7441 ccctcgtgac ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt
     7501 tacaccaacg gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag
     7561 cagagaaatt tgtgctactc caacatgacc agaacaatca gcttaatatt tagcaacatt
     7621 tggtgattaa gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc
     7681 gattaatacc atgaacgcta aaacgcacag cggaaaatgc cagggtcagc cataagtaac
     7741 gcaaaaaaag atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa
     7801 tggatagtga gatatggggc gcaaatttgg cagggaaatg tgtggtgtgt aggtcgaata
     7861 aggcggtcgc gccgcatccg acaataagtg cggtgccgt
//
LOCUS       DAHBHC010000142         7595 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHBHC010000142 DAHBHC010000000
VERSION     DAHBHC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.145"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(55..1284)
                     /gene="pepT"
                     /locus_tag="M2A35_004720"
     CDS             complement(55..1284)
                     /gene="pepT"
                     /locus_tag="M2A35_004720"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCB7200917.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCNIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            1534..2670
                     /gene="potA"
                     /locus_tag="M2A35_004721"
     CDS             1534..2670
                     /gene="potA"
                     /locus_tag="M2A35_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCB7200918.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2654..3511
                     /gene="potB"
                     /locus_tag="M2A35_004722"
     CDS             2654..3511
                     /gene="potB"
                     /locus_tag="M2A35_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCB7200919.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            3508..4302
                     /gene="potC"
                     /locus_tag="M2A35_004723"
     CDS             3508..4302
                     /gene="potC"
                     /locus_tag="M2A35_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCB7200920.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            4299..5345
                     /gene="potD"
                     /locus_tag="M2A35_004724"
     CDS             4299..5345
                     /gene="potD"
                     /locus_tag="M2A35_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCB7200921.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            5403..5864
                     /locus_tag="M2A35_004725"
     CDS             5403..5864
                     /locus_tag="M2A35_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HCB7200922.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            5861..6649
                     /locus_tag="M2A35_004726"
     CDS             5861..6649
                     /locus_tag="M2A35_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCB7200923.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(6788..>7595)
                     /gene="cobB"
                     /locus_tag="M2A35_004727"
     CDS             complement(6788..>7595)
                     /gene="cobB"
                     /locus_tag="M2A35_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCB7200924.1"
                     /translation="GHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVLVLTGA
                     GISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQPEIQPN
                     AAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQVLDWT
                     GDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGHVYPAA
                     GFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
BASE COUNT         1888 a         1913 c         1830 g         1964 t
ORIGIN      
        1 atcaatataa cggtttcatc ccggtaattg gcagtccaca tacgcatcgg gcaatcacga
       61 cttccgttgc gccgttaact cggcaatacg gacaatcacc tgcaccgctt tttccatacc
      121 ttccagagtc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
      181 gcacggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
      241 ttccggttca atattgcagt cacgcatcgc ctgctgggcg atatcgataa tatgcggatg
      301 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccagtt caatgtagca
      361 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
      421 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggctc ggtcaacggt
      481 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
      541 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
      601 tttcgccgtg cccggatgaa cattgttacc gacaattttg atattgaccg atgcggcgtt
      661 gaagttttca aactccagtt cacctacgcc accaccgtca acagtgtaag cccagcgggc
      721 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
      781 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
      841 gatttctgca ataccggctt tgtcatcggc acctaacaag gttttaccat cggtagtaat
      901 cagcgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
      961 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattcacatt
     1021 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
     1081 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
     1141 gataagcccc atctcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
     1201 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
     1261 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
     1321 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
     1381 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
     1441 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
     1501 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
     1561 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
     1621 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
     1681 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
     1741 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
     1801 tatgtgaaca ctgttttcca aagctacgca cttttccccc acatgaccgt gttcgaaaat
     1861 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
     1921 gaagccctgc ggatggtgca actggaaacc ttcgctcaac gtaaaccgca tcagctctct
     1981 ggtggtcaac agcaacgcgt cgccattgcc cgcgcggtgg ttaacaagcc tcgtctgttg
     2041 ttgctggatg agtcgctctc agcgctggat tacaaactgc gcaagcaaat gcagaacgag
     2101 ctgaaagcgt tacagcgtaa gcttggcatt acattcgtct tcgtgactca cgaccaggaa
     2161 gaagcactca ccatgtcaga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
     2221 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
     2281 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
     2341 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
     2401 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
     2461 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
     2521 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgac
     2581 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
     2641 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
     2701 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
     2761 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
     2821 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
     2881 cctcgcctgc ctggtgctgg gctacccgtt tgcctggttt ctggcgaagc tgccacacaa
     2941 ggtgcgtccg ctgctgctgt ttctgctgat tgttcccttc tggaccaact cattgattcg
     3001 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
     3061 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
     3121 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
     3181 ggataagcct ttgcttgagg cggcgcgcga tctcggtgcc agcaagttgc agacctttat
     3241 ccgcatcatt atcccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
     3301 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
     3361 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccatttg gtgcagccac
     3421 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
     3481 tctgctgaat aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     3541 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     3601 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     3661 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
     3721 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
     3781 ggtaagccgt tcgttagcgg aatgctgttt gtggtgatga tgtcgccaga tattgtgatg
     3841 gcgatttctc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     3901 ctgttctcgc atatcacctt ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg
     3961 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag tgaatttacc
     4021 attctgcgaa aaatcattct gccactggca atgcctgcgg tggcggcggg ctgggtgtta
     4081 agctttaccc tgtcgatgga cgatgtagta gtttcttcgt tcgtcaccgg accaagctat
     4141 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     4201 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg taattgccag ccagcttatt
     4261 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     4321 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaata
     4381 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     4441 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
     4501 agttgaaaac gtacaaagac ggtgcctacg acctggtggt tccttcaacc tattacgtcg
     4561 ataaaatgcg taaagaaggg atgatccaga agatcgacaa gtcgaagtta agcaatttca
     4621 gcaacctcga tccagacatg ctcaacaagc cgtttgaccc gaataacgac tactccattc
     4681 cgtatatctg gggggcgacg gcgattggcg ttaacggtga tgcggtagat ccgaaatccg
     4741 ttaccagctg ggccgatctg tggaagcctg agtacaaagg tagcctgctg ctgaccgacg
     4801 atgcccgtga agtgttccag atggcgctgc gcaaactggg ctactccggt aacactaccg
     4861 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     4921 cgtttaactc cgataacccg gctaatccgt acatggaagg cgaagttaac ctcggcatga
     4981 tctggaacgg ctctgctttc gttgcacgcc aggcgggtac gccaattgac gtggtgtggc
     5041 cgaaagaagg cggcattttc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     5101 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     5161 ccgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agcccggaag
     5221 tggcgaacga taaaacgctc tacccggatg ctgaaaccat taaaaatggt gaatggcaga
     5281 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
     5341 gttaattcag caaacgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
     5401 taatgagtca ggacagtaaa gttttatccc gcgcttttct cggtatcggt ctgatattgc
     5461 tcgttatttc tgcaatcatc ttttattacc attttacttt taccaaaagc gcggttcata
     5521 cagaaggaat cattgttgat gccgtttggt acaacaatca ctcaaacgac gtcgatgata
     5581 atggttcctg gtatccagtg gttgcctttc gccctacgcc agattacacg ctaattttca
     5641 attcgaatat cggtagtgat ttttatgaag atagcgaagg tgacagagtc aatgtctatt
     5701 atccgccagg gcatccagaa caagccgaaa taaacaatcc ctgggttaat ttttttaaat
     5761 ggggctttgt tggcatagca ggcattattt tcggtagtgt cggtctgata atcacgctcc
     5821 cctccaccaa aaaatctcgc aggaaacgga agtcccgccc atgaaaaaat tcattatact
     5881 gctcagttta ctgatactcc tgcctctggt agcaaccagt aaaccattga ttccaataat
     5941 gaagacactt tttacggatg ttaccggtac tgtaccggat gctgaagaga tcgcacgtaa
     6001 agccgagctt tttcgccaac aaacgggaat tgcaccgttt attgtgattt tacccgacat
     6061 taataatgaa gccagtctca gacaaaatgg taaagcgatg ctggcgcatg cgtcatcttc
     6121 attgagtaat gtaaaaggaa gtgttctgtt actatttact acccgcgaac cacggttaat
     6181 tatgatcacc aacggccagg ttgaaagtgg tctggacgat aaacatctcg gccttctgat
     6241 agaaaatcac acgctggctt atttaaatgc agatctctgg tatcagggaa tcaataatgc
     6301 attggctgtt ctacaagcac agatattaaa acaaccgacg ccgccactga cgtattatcc
     6361 gcatccagga caacagcatg aaaatgctcc ccccggcagc accaacacat taggttttat
     6421 cgcctgggct gcgacgttta tcttattttc ctggatcttt tgctacacga ctcgttttat
     6481 ttatgcgtta aaatttgcag tggcgatgac tattgccaat atgggttatc aggctttatg
     6541 tttgtatatc gataatagct ttgcgattac cagaatatcg ccactctggg cagggctaat
     6601 tggtgtctgt acgtttattg ccgcattgtt attgacgagt aaacgataaa agaggccgga
     6661 aaagtgcgcg tgtattatgc cggatggggc gcgaacgcct tatccggccc acaaaacccg
     6721 caaattcaat taattgcgtc cccttgtagg cctgataagc gtagcgcatc aggcaatgct
     6781 tcctgcttta taatcccttc agcaactttt caacaaactc aggcaccacc tggcttgccg
     6841 ggccgtaata tttctcggca aattcattac cgacctgact tggttcgaga ttcagttcca
     6901 cggtgtgcgc gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc
     6961 cggaagtacc aatggcaatg aaaatatcag ccatcgacaa cgccatataa atttcatcca
     7021 tgccgagtgg catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc
     7081 aacaatggca tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt
     7141 gcgaacaacg aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc
     7201 gttcatgcag gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt
     7261 gcagtttagc cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc
     7321 gacgacgggc gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg
     7381 ttgccacatc ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa
     7441 tacctgattc cgcagaaatc cctgcccctg tcagtacgag tactcttggt ttttccattg
     7501 cttccggcac cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc
     7561 gtttattttt acgaaaacga cttaaccgat gaccc
//
LOCUS       DAHBHC010000143         7589 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHBHC010000143 DAHBHC010000000
VERSION     DAHBHC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.146"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            870..2015
                     /locus_tag="M2A35_004728"
     CDS             870..2015
                     /locus_tag="M2A35_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspG"
                     /protein_id="HCB7200925.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
     gene            2162..2979
                     /locus_tag="M2A35_004729"
                     /pseudo
     CDS             2162..2979
                     /locus_tag="M2A35_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
     gene            3204..4052
                     /locus_tag="M2A35_004730"
     CDS             3204..4052
                     /locus_tag="M2A35_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HCB7200926.1"
                     /translation="MSASVSIPAEQSQSISTIPGGRKIETVPEKEGLLVFLGTKSVGE
                     YALNILGQNVSRVTTGKKPYDILFLHEATKQDFDKKKTEFTFPGANRSYLQSSNTDVA
                     AAAAISIAATEMKTILPKDLTPEKYNKIYLPGDGSAGLPLLKCGDEFLSPTDIVNRLV
                     EHNLHEVEDIRLTSCHSANITKNNDFSPEEIEKSTKTNSGWLASALFGQKRSLAEHVY
                     AEFEGRGINVSVSGYHGTGVFYVPEHGKPTTHLRSTTVPATPEQTVRRSDYRVSLGRT
                     QPTDID"
     gene            complement(4292..4471)
                     /locus_tag="M2A35_004731"
     CDS             complement(4292..4471)
                     /locus_tag="M2A35_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCB7200927.1"
                     /translation="MTIIEHTDVDESLKGQGIGKRLVAKVVEKMRREKRKIIPLCPFA
                     KHEFDKTREYDDIRS"
     gene            4553..5107
                     /locus_tag="M2A35_004732"
                     /pseudo
     CDS             4553..5107
                     /locus_tag="M2A35_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
     gene            5279..5575
                     /locus_tag="M2A35_004733"
                     /pseudo
     CDS             5279..5575
                     /locus_tag="M2A35_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            5662..6096
                     /locus_tag="M2A35_004734"
     CDS             5662..6096
                     /locus_tag="M2A35_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCB7200928.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            6093..6203
                     /locus_tag="M2A35_004735"
                     /pseudo
     CDS             6093..6203
                     /locus_tag="M2A35_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            6268..6528
                     /locus_tag="M2A35_004736"
                     /pseudo
     CDS             6268..6528
                     /locus_tag="M2A35_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6593..>7589
                     /locus_tag="M2A35_004737"
     CDS             6593..>7589
                     /locus_tag="M2A35_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HCB7200929.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQHNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGL"
BASE COUNT         2421 a         1662 c         1554 g         1952 t
ORIGIN      
        1 ctacagcact aagacgttcc gctatatagc tattacctgt tcccctgaca taaggagcag
       61 atatacctca accccctatt ccccagtgct gaccgggagc ggcggcggta taaaaaggaa
      121 caaggaacaa ggaacaagga acaaggaaca aggaacaaat ggcagaacaa gcaaccatgt
      181 ttgtcccccc cgcataaccg taccattcgc attaagaaat attcccatat ctatcccgca
      241 aaagaggacc cgtcatacat caagtccgtt ttactgggca tcagattatc gccgtaatta
      301 gctcagtgaa agccgggtaa acggttagca agctcagtgg ctcaaaagtg catagcgcta
      361 agatgggcct ggtgacacct gccctttgct gcagatcgtg ttgatcggtg tgatgccgcc
      421 acacgtttaa gtcaatctgg aaaactcaca atgcgctctt ctataaagag gactctttga
      481 taaagtaacc cgaaaaggga aagctaactg atatcaatgt accaccttag taatcatccc
      541 ttatatacag acaaaaagaa gttgcctgaa agccaggtat ctaaactaat cactaatggt
      601 atattttatc agcacaataa aaattttttc tgaatgctat tattaatgta aataattaca
      661 accttttcgt aggcacaaaa actattgctg atgaatcaat gaaaatctat tgttcgtcat
      721 tagaggagta tttcctctaa tttaaaaaaa taactctcta tacatttaaa actctcgcta
      781 taaatagctt atatattatt gacactatat catgctatgt gatatctgtt gtcgaatcat
      841 tcataataaa tgagacaggg gaatctctca tgataaacgg acttaataat aattccgcat
      901 ctttagtttt agatgctgca attagaatta attctgattt taaaaagcag tggaatgata
      961 tgtcatgcgc tgaaaaatta ctaaaagttc ttagttttgg tttatggaat ccaacgtata
     1021 ctcgtagtga aaggcaaaca tttcaagagt tgttaaccgt tttagaacct gtatctcccg
     1081 cgcctaatga attgggcaga atatatgcta attttgcaga tggctcatcc ttaagaattt
     1141 ccgtcactaa cagcgaactt gttgaagccg agattcgcac accagataat gaaaagattc
     1201 ttatgctcct ggagtcaaac gaacaaaata ggttattaca atctttaccc atcaatctcc
     1261 acatgccata cattcaggtt catcgtgcct tatctaagat ggacctgact gatcataagt
     1321 caatgcacaa tctacttagt tttacgtcaa aactatcagc aaccttgatt cctcataata
     1381 ctcaaacaga tccgctttcc gggcctacac cattcagctc tatgtttatg gatacatttc
     1441 gaggactcgg caatgcaaag ctttcactca atggtgttga tatacctgta gatgcacaaa
     1501 aattgcttcg cgatgcacta ggacttaaag acacacattc atcacttgcc cggaatgtta
     1561 taaataatgg tatttctcgt catcatgcag agcaaatagc aagggaaagc agcggcagcg
     1621 ataaacagaa agctgaagtt gtggagtttt tatgccaccc cgaagcggca acagccatat
     1681 gctcggcttt ctatcaatct ttcaatgtgc cagccttaat gttgacacat acaaggatct
     1741 ctcaagccag agaatacaat gtggaaagat cgttagatgt acctaacgcg tgcattaaca
     1801 taagtatctc tcaatcacca gatggaagca ttcacgttgc cagccatacc gggattctga
     1861 taatggcgcc agaagaccgc cccaacgagt taggaatgtt gacgaacagg acctcctatg
     1921 aagtgccaca aggtgtgaaa tgtgaaatcg atgagatggt aagaacgcta caaccaaggt
     1981 atggcgcatc tgaaacatac ctgaaaaaca tttaaaaata aaaaaatgga caccacacac
     2041 tttatttatc aaaacaaaca ataactaatc acactcacaa ttattaataa tttaacctca
     2101 cagcaatcat acaaaacaaa gatatttatt tcgcaaacca tttatagcga atggagaagg
     2161 gatgaaaaga agattattta ttgcattttt tattgcaatg tttgtcacac caattgcatt
     2221 aatattattt gtatttgatt atggttacaa aaaatcacca caggaatact cactttcatt
     2281 tataaaacaa ttaccaaata cggtgaactt atcatctctt acgtataata aagaggatga
     2341 tttcctgtat gccacccaga atagccctgc gcagctactt aaaatagcca gaagtggcga
     2401 catcatcggc cgagccccgc tgccttttat ctctgatgcc gaaaccattg agcacataca
     2461 agggaacatt tttgctgcag tagacgaaaa gacttctgaa ctattctttt ttacagttac
     2521 aaaagatatg catatctcct tgcgaaataa aattcagtta gaaaaattca ataaaaaaat
     2581 agagggtttg aaggactggc atggaaagca gaaggccgga tgctgtttgt cgctaaagaa
     2641 cgacgcccta caggcatgtt tgcctatcag ctttctccag atctgctgag tgcaaaacaa
     2701 gtaacgattc cagaggaact aaatgatata catgttaaag atatatccgg cctggacttt
     2761 aataatgaaa gtctgatgat tctgtccgat gaatcgcgta agttactcaa gtttaacctg
     2821 acagaaatgt ctttcgttga gatgatggac ctgacaaaag gaaatcattc tctgaccagt
     2881 gatttgctcc aacctgaagg cattgtaact ctgcctgatg aaagcattta tgtcgctagt
     2941 gaacctgata tcctcgctaa atttgtccct aataaataag tctgtcacta gccttagtat
     3001 tgagcgacca aatctggcaa tctcatgaaa ctcgcaaaat acgaatctaa tattaataaa
     3061 aattttactc tgaaataaac agattaatag ctgaaaagga tatcagctat tatcaactaa
     3121 aatattactc actccattag aattatcatg aactcacatt taaacaaact aagaaggagg
     3181 ttgtatgttt agtataacta cctataagcg ccagcgtatc aattccggca gaacaatctc
     3241 aatccatctc cacaattcct ggagggagaa aaatagagac agtgccagag aaagaaggat
     3301 tgcttgtgtt tttaggaaca aaatccgtag gtgaatatgc tcttaatatt cttggtcaaa
     3361 atgtttcaag agtcacaaca gggaaaaaac cttatgatat attattcctg cacgaagcta
     3421 caaaacaaga ttttgataaa aagaaaacag agtttacctt tcctggtgca aacagaagtt
     3481 atctacaatc aagtaacacc gatgttgctg ccgcagcagc tataagtatt gcggcaacag
     3541 agatgaaaac tatcctacca aaggatttaa caccagaaaa atacaataaa atttatctgc
     3601 caggggatgg ttctgctggc ctacctcttc ttaagtgtgg agatgaattt ttatcaccaa
     3661 ctgatatcgt caatcgtctg gtagaacata atcttcatga agtagaagac atcagattaa
     3721 catcctgtca ctcagccaac ataacaaaaa acaatgattt ctctcctgaa gaaatagaaa
     3781 aatcaacaaa aaccaacagc ggctggttgg ctagtgcatt atttggtcaa aagcgttcat
     3841 tagcagaaca cgtctatgcc gagtttgaag gccgtggaat taacgtttct gtatccggtt
     3901 atcatggcac tggtgttttt tatgttccag agcatggcaa accaacaacg catctacgct
     3961 ccacaacagt gcctgcaaca cctgaacaga cggtaagaag aagcgactac agagtctccc
     4021 ttggcagaac tcaacccaca gatattgact aattgagtct gcatataggt ttgtgtcatt
     4081 acctgatgcg ctacgcttat cagacctata ttagatcctg caatatgttt aatttgtagg
     4141 cagaatagag cgttcacacc acatcaggta taaacaaagt acagcttgcc aaaaacctga
     4201 ataaccccga atttacgggg ttattccgct taaacatgcc cataacatca cttctgattt
     4261 gtattgctta cgacggatac aatttctctg ctcagctacg aatatcatca tactcgcgcg
     4321 ttttatcgaa ctcgtgtttc gcaaacggac atagcgggat aatcttccgc ttctcccggc
     4381 gcattttttc caccaccttc gccaccagcc gtttaccaat cccctgccct ttcaggcttt
     4441 catcaacatc ggtatgttcg ataatggtca tagactggcc ccctgaatct ccagacaacc
     4501 agtatcactt aaataagtga tagtcttaat actagttttt agactagtca ttggagaaca
     4561 gatgttgagt tatgactcgc attcatcagg gcgagtctat tccagagaag aatatatgat
     4621 gtgggaattg cagcaacggg tatcagaagc ttccagtgcc cggacacagg attactggct
     4681 gatggatgca gcggtaagaa acggagaatg gaagatcaca ccagaattat tacgtcacac
     4741 accgggatat atccggagta cggtatcgaa atggtccaga ggatggctga aaaccggcac
     4801 aatactccag actccagaag acagaaatac ggatgtatac ctgactacca tacagaacaa
     4861 tgtatttagt cgtcaggggg gcggctacca agtgtattat cggattgatg gaatggctgg
     4921 tgcggatata gcggataatg caccagggga aacccgctgc accctcaggc ccggaacatg
     4981 ttttgaagtg acaagtgtgg atgaaaggca ttatgagtgg aatatcattt atgtcacgct
     5041 gaaaacctgt ggctggagcc gaaatggcca aagcaaaacg ccgaatggtg acaacctttt
     5101 taactaatta aaatctctgt taaagatgta taacccggca tctatgtgct caccagcata
     5161 tagatgcaat gtgattcccc tgatggtcgt atcagagatg gtatgcgagc ctgtacatag
     5221 atttgtgtaa ttgcctgatt ttgatatgtt caatccaaca tcaaaagcag gttaatttat
     5281 ggacgaaaaa cagttgcagg ctctggctaa cgaactggcc aaaaatctca aaacccctga
     5341 agatctcatc cagttcgatc gcctgctgaa gaaaatcagc gttgaggcag ctctcaacgc
     5401 tgaaatgtcc caccatctgg gctacgataa aaaccagcct aaaccgggtg ccaactcccg
     5461 caatggctat tccacaaaga ccgttatcat cggtgatggc cccctggaac tacgcactcc
     5521 gcgcgatcgt gatggctctt ttgaaccgca acttgtgaag aaaaaccaga cccggagtaa
     5581 gcgtaaagcc aatgccgtct gtaacacctg ctccttgcag actaaattag agctccttct
     5641 aaattagacg gagttctatt gatggataaa cctacagact ggcgctccgg aacccgccgg
     5701 atattttcta atgaatttaa acttcatatg gttgaactgg cttcgaaacc aaatgccaat
     5761 gttgcacaac tggcccggga acatggcgtt gataacaacc tgatttttaa atggctacgc
     5821 ctctggcaaa gagaaggacg tatttctcgt agaatgcctc caactattgt aggccctaca
     5881 gtatcacaat cttttccggc ctctccgact ctggttccag tggaacttat cgacaccccg
     5941 cgctgtgcta cagatgctcc tgctccggag gcattatcag ttgcttgtgc agcttcctgc
     6001 catgtggaat tccattacgg taaaatgatg ctggaaaatc cttcaccaga gctgctcacg
     6061 gtgttgatcc gtgagctgac cgggagggga cgatgatttc actcccatca ggtacccgta
     6121 tctggctcgt tgccggcgtt actgatatgc gtaaatcctt caacggtctg ggggagcaga
     6181 tacagcatgt gctggatgat aactgaggta gcctgagttt aacggacact ccttcctgaa
     6241 atagaatggc atcagaagga gctaataatg agcagaaaaa cccaacgtta ctctaaagag
     6301 ttcaaagccg aagctgtcag aacggttctt gaaaatcaac tttcgatcag tgaaggcgct
     6361 tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc cagaaaaggg
     6421 ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct gcaactgcgt
     6481 aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac tgtagattca
     6541 attggtcaac gcaacagtta tgtgaaaaca tggggttgcg gaggtttttt gaatgagacg
     6601 aacatttaca gcagaggaaa aagcctctgt ttttgaacta tggaagaacg gaacaggctt
     6661 cagtgaaata gcgaatatcc tgggttcaaa acccggaacg atcttcacta tgttaaggga
     6721 tactggcggc ataaaacccc atgagcgtaa gcgggctgta gctcacctga cactgtctga
     6781 gcgcgaggag atacgagctg gtttgtcagc caaaatgagc attcgtgcga tagctactgc
     6841 gctgaatcgc agtccttcga cgatctcacg tgaagttcag cataatcggg gcagacgcta
     6901 ttacaaagct gttgatgcta ataaccgagc caacagaatg gcgaaaaggc caaaaccgtg
     6961 cttactggat caaaatttac cattgcgaaa gcttgttctg gaaaagctgg agatgaaatg
     7021 gtctccagag caaatatcag gatggttaag gcgaacaaaa ccacgtcaaa aaacgctgcg
     7081 aatatcacct gagacaattt ataaaacgct gtactttcgt agccgtgaag cgctacacca
     7141 cctgaatata caacatctgc gacggtcgca tagccttcgc catggcaggc gtcatacccg
     7201 caaaggcgaa agaggtacga ttaacatagt gaacggaaca ccaattcacg aacgttcccg
     7261 aaatatcgat aacagacgct ctctagggca ttgggagggc gatttagtct caggtacaaa
     7321 aaactctcat atagccacac ttgtagaccg aaaatcacgt tatacgatca tcctcagact
     7381 caggggcaaa gattccgtct cagtaaatca ggctcttacc gacaaattcc tgagtttacc
     7441 gtcagaactc agaaaatcac tgacatggga cagaggaatg gaactggcca gacatctaga
     7501 atttactgtc agcaccggcg ttaaagttta cttctgcgat cctcagagtc cttggcagcg
     7561 gggaacaaat gagaacacaa atgggctaa
//
LOCUS       DAHBHC010000144         7559 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHBHC010000144 DAHBHC010000000
VERSION     DAHBHC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.147"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1073..2557
                     /locus_tag="M2A35_004738"
     CDS             1073..2557
                     /locus_tag="M2A35_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCB7200930.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQFCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2879..3481)
                     /locus_tag="M2A35_004739"
     CDS             complement(2879..3481)
                     /locus_tag="M2A35_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200931.1"
                     /translation="MKQQYQTRYESLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWISFNGEKPEIAIVPQSLHRLIYGPDKRSTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(3576..3782)
                     /locus_tag="M2A35_004740"
     CDS             complement(3576..3782)
                     /locus_tag="M2A35_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCB7200932.1"
                     /translation="MNTPVSLMDDQLVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            4497..4653
                     /locus_tag="M2A35_004741"
                     /pseudo
     CDS             4497..4653
                     /locus_tag="M2A35_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(4935..5129)
                     /locus_tag="M2A35_004742"
     CDS             complement(4935..5129)
                     /locus_tag="M2A35_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200933.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVAPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            5329..5898
                     /locus_tag="M2A35_004743"
     CDS             5329..5898
                     /locus_tag="M2A35_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCB7200934.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            6066..6449
                     /locus_tag="M2A35_004744"
     CDS             6066..6449
                     /locus_tag="M2A35_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200935.1"
                     /translation="MYAKSFLALDGNGRLTGARTAHTAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERKEIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            6446..6871
                     /locus_tag="M2A35_004745"
     CDS             6446..6871
                     /locus_tag="M2A35_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200936.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            7116..7313
                     /locus_tag="M2A35_004746"
     CDS             7116..7313
                     /locus_tag="M2A35_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCB7200937.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDILDINDPDFPEQYQRLLARL
                     DYLTKLDTKASGQRNSGYSGF"
BASE COUNT         1906 a         1684 c         1956 g         2013 t
ORIGIN      
        1 tccatgaaaa tacgccgtta ttgttaaaca gcaggctgat ttctccgtaa aggggaaaat
       61 atttatcggc tgtatgatta tcaggtgtgt ctgtgtttac tgctgtgagt attttttagt
      121 gtattgtggg ggagatgtca gtgggagata ttaaatttac gtttataatt atcttacttc
      181 ttctgtgtgt tttttataag agtgcacacg taatgatatc tgttgatacc atgtggttaa
      241 gtgtttccct tcctagtcat gcgtaatgat taaatgcttt tgtgacacca ttcttccggt
      301 ctgaatattt atcccgttct tttttatttg tgcagtgata ctgtcggaag agaattcagt
      361 aatgtgaggt gtcctggtca ggataatatc tgctgagata ttattagttg agtgtgtttt
      421 tactgagcaa aagctggctt ttaattcctg tactgaaaca ctgtcgctcg tccggggtta
      481 actgaaactg gatgatgatg taaaatgaag gctgtttttg tgtttgtcga agaagtgtta
      541 ttttaatcca ttctcatcat tggctggcga ttatacagaa gtatttgaag aaaaaatggt
      601 gcattttatc tgtgtcagca gggaggaagt attttattga tgtatcagta ttaaatggtc
      661 tttgttattt ataaatatgt catcctgtcc atagaatatt gtactcagca ttcagtgcag
      721 gaactgtagt cttctttatt ttattcttct tcaggagggg ggggattcct gaaggcgtta
      781 ttcccggtgt tctgtttatg attcttcccg gcaggaaacc ggtatctgca atacccggga
      841 tgatgctttt tgttcgtacg tggttgtgta aaatttgaat agtggatgtg ctccgaaggg
      901 aaggagggga cacacagatt cattttgtct ttcttcagca gcaaaacaca ctgtggttat
      961 tatttaacca ttaaatcaac aaaaattttt acctcatatt gatttgactg ctatttttgc
     1021 tattaatagc cagaattgca gtgcttttat atctgtgtgt agtgaggtta ctatgtcatt
     1081 cggtcagcct tgtgatgagt tccccctgag ttcgctgcct ccactcattc gtgatgccgt
     1141 tattgaagct cagcaaatta cgcaggctcc ccttgggctt gtggcagcgt ctgccctggg
     1201 cgctgtttca cttgtctgcc agaatctgat tgatgtctgc agactgaata cactgcgggg
     1261 gccggtctct ctttttttcc tgacgcttgc cgagtccgga gaacgaaaaa ccgcagtgga
     1321 caaattatta atgaagcccc tgtaccagca ggagatgcag ttatattcca gatacaaaag
     1381 tgaactggct gtctggaaaa acaaggaaga gcttttaaag gctcagaaaa aagcattgtt
     1441 gtcaaaactg aataaagaac tccgcaaagg cgcggatgag tcagaaaccc tcagacaact
     1501 ggaagtactt cagaaaaaca gtgcggaaga acctgtgagg tataaattca tttttaatga
     1561 tgccacgact gcggcaatta agaatcagtt ttgtggtaaa tggcgttcag ttggtattat
     1621 gtcagatgaa gccggtatta tatttgatgg atataccctg tctgagttgc cttttattaa
     1681 caaaatgtgg gatggctctg tattgtctgt ggacagaaag aatgaacccg aacagatgat
     1741 tgaaaatgcc agaatgacgt tatccctcat ggtgcaaccc ggtctttttg accgatatat
     1801 ggagcgaaaa ggttctgttg ccagggacag tggttttctt gcccgttgtc ttatcagtaa
     1861 acctgcaacc acgcagggga aacgctttat taacggagct gtgattccgg gaggatcatt
     1921 aacggcattt catgaacgat taatggaact ggccagaggc agtattgaaa aatccagtga
     1981 ggatgaacgg tattgtctgc atttttcgcc tgaagcgcaa aaaatattta ttgagcacta
     2041 taatgttctg gagcaggatt tatccccttc cggaccctta agcccgtttc ggggacatgt
     2101 ttcaaaaaaa acagaaaaca ttgcaagaat tgcagcgtta tttcagtatt tcagctatgg
     2161 tgaagggaaa atctctgccg atatcatgac gtctgctgtc gtaataagtt cgtggtacac
     2221 agatgaatat aaaaaattat ttgcgttacc tgatgaatct gaactgcagc agaaggacgc
     2281 cgaggagctg ttcgactggc tcattgagga gtgccgcgga gaatgcccgc cccgtgtcag
     2341 gaaaaattat attctccagt gtggcccggg ccggttcaga aacaggaaaa aactgaacgc
     2401 attgctgaac attcttgaat ctcagttcag gctttctgtc gtgccggaag gaaaaaccat
     2461 gtatgttctg cttccccaga tagccagttt aaagttgtct gatgtctctg gtatttttac
     2521 ctcgggttat cactacaata agctgagagc gaagtaaccg gtgagcagtt tttctccatt
     2581 atgcttcaga cttagatgat tgattattat ggggagttca tcaccgatat tcagatgtat
     2641 gaatatcggt gatgaactaa agctcaggag ctgtgtggtg gtgatgaatc aacaggcata
     2701 taactgatac ccgccggtca gaagttccag cctgcgactt tcctgtctgc gtgcccgcag
     2761 tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga gcgaaacggc aggcagacag
     2821 gccgttcagt cagccctggc accctgcccg gataccagct tccctgaaac aactggcatc
     2881 aggtgcgctt atcgtcaacc gttgtgcggt atgccaggag cacgtactga tccatcagat
     2941 ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca ttcctccagc tctttttctg
     3001 atttccgttt cagattctcc gtccagtcgt caaggcagtt tccggtcatt aaatcaagcc
     3061 agcaaagcca gaccagtttg aggcgcaact ccgtgccacc aaactgacgg aagaggctga
     3121 tcagtttatt tgccagcgaa cgctgcctca gacgttcaca ttcagacagg agaatgcctt
     3181 ccagcatcgg atgatgaacc agcagatgtt cactggtgca taaattcact atcagatcat
     3241 catccagggg cggcgtgctc cgtttgtccg gaccataaat cagccggtgc agactctggg
     3301 gtacgatggc gatctcgggt ttttcaccat tgaatgatat ccatccgggc gtcagattat
     3361 ggaagtagta gatattcgga tgacacactc cccaggatac agcgtgccgg gtgaagccgg
     3421 tcagccattt ctggtagctt tcgtggagcg attcgtagcg ggtctgatat tgttgtttca
     3481 ttttcatctt cctctcgggg atataacagg cagcagaatg ccgccgacag gccggaaatt
     3541 gcgtctgctg caggtaaacg gataacggtt atgaactacg gacgggactg tgcaatgcgt
     3601 gcctgcagcc aggcttccac ttcgcttttc agccagcggg agctgcgacc cattttgata
     3661 ggggccggaa aggcaccatc tctgatgagc ttataaaacc acttatcggt taagccggtc
     3721 agttgagtga taaatgccat gtcgaccagc tggtcatcca tcagcgaaac cggggtattc
     3781 atgaaaatct ccttcggatg ttgttaatct caaccgacgt ttgtgatgaa aacaacgcat
     3841 cacctgcacc ggcataacaa ccggggagaa acgtaaaaaa tgagtagggg aatgttcctg
     3901 ccgggagata aacagggagc atgatgctga gatacccggt ggctaccgcg aaacgatgct
     3961 tctgcacgca cgcgcgataa agaagggatg attttcgtga ggatggggga aaaaccggcg
     4021 ttctgataat tgcagagttg ttttgggagg cggttacagt gagtcagtct gttcctagcg
     4081 ggcaggcgac aggtatcacc gatgggataa gactgtctga ttggtattat aaatatataa
     4141 gagagatcaa acacatgagt tcatatctgt gaaataaaca ctgtgagtgt gatcagtggg
     4201 catgagaacg cagtagcaac agattacgtt gtactcacag ggctatccct gatagctggc
     4261 gtataacctg cgtatataaa acgtacctgc aacacacgtg aacagagtgc attaagcatc
     4321 cgtctgagcg gtaaacgtat ccgacctggt gagtgacagt ctgagagacc ggttaagtgg
     4381 tggtacggga gtaatgagtt tggggtgggt accagaatgc cagtctggtg cggtatgctg
     4441 gcagtatcac gttaattgca gcataaagtg atgagaggct aagtgatgag aggctaatgg
     4501 aagttaaaac caaagaagac tggctgtatc agttccgtcg ttgttcatcc cgggagacac
     4561 tggaaaaagt gattacccgc acacgttata aacttacccc tgcggagctg gaagccttct
     4621 gcggtcgatc ggctggcaga actgacgaca aactttacgc gtgtggagcc agtctggtca
     4681 cctgagtatc cggtaatgac aacggtctgg tgttatctgc tctgtgtgat tctttttagg
     4741 tcttctccgg ttgttggtaa cctggataat accgcgaacg ttaagcggta ttgtgacaac
     4801 gaataatttg aaattaaaag gggcaggggg ggggctgctt cgggtattaa cggcttacag
     4861 cgtgtgggtt gacggcgcgt tgcaggggac gaactctggc aaccctcacg gatatctgat
     4921 aagccccgtt accgctacca cagcacatac atgctgacag cagatgtcac cagcaaaacc
     4981 acaaccagca gcgccagcag ggctgtgaag atcctgtccg tattccggta atagccgaag
     5041 ggggcgacag aaccacagta cggacaacac accgtattat gagtaatcgc gttaccacag
     5101 tccctgcact tctttttgcg tatcaccatc actccctcct cacagcctta cccgtaaccg
     5161 gttttttaca ttaaaaaaac aacgctgaaa attcaaccgt cagtccggag acgaccgttc
     5221 gggctatcac aaagtgcctg agacagtgtc ctgccggagg tcacttcagg gactctgcgc
     5281 attttttacg acgtggctat tccgtatgaa ccatacggag atttaaccat gacctacaaa
     5341 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
     5401 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
     5461 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
     5521 cgtaagcatc gggaaggtaa acgcgtgcat cccaccactc tgcattacgt ctgggcccgg
     5581 gagtttgggg agtgcaaagg taaaaaacac tatcatctga tgctgctggt caaccgggat
     5641 acctggtgtc gtgccggtga ttaccgcgct ccgggatcac tggccgggat gattaaacag
     5701 gcgtggtgca gtgccctggg agtggatacc ggagatcata ccacgctggc gcattttccg
     5761 gtcaggccgg cggtgtggct ggaacgcgat gatgacaccg gctttcagca ggtgctggag
     5821 cgtgctgact atctggcgaa ggagagtacc aaagcttacg gcaccggtga gcgcaacttt
     5881 ggctgcagtc gtggctgagc ccgacagacg acttatctga ccttagtgtg atgacgtgtt
     5941 cacagcgacc acactgattt tcccttctga gactgaactg aaaaccgcca ttcacacctg
     6001 accacgctgc ccgtaccggg acgactgttg cctgcgtgtc gtctggcggc tgaggaatat
     6061 atactatgta cgcaaaatcc tttctcgcac ttgatggtaa cggacgtctg acgggggccc
     6121 gtaccgcaca tactgctcct tatgcccact acacctgcca cttgtgtggc agtgcactca
     6181 gataccaccc gcaatacgac accgaactgc cctggtttga acacactgac gacgggctga
     6241 cagagcacgg tcaacagtgt ccttatgtca gaccggagcg caaggagata caactgatta
     6301 aacgtctgca gcaattcgta ccggatgcct tacccgtagt acgtaaagcc agctggcact
     6361 gcaggcaatg tcaccacgat tattatgggg agcggtactg cacacactgc cagaccgggc
     6421 gcttcagtga ggagggggga gcagaatgaa cggtataaaa acggggctgg ggatcacgcc
     6481 gggtgagcac attattagtg ccgactctgc gctcagcaga aatatccggc agtgtttctg
     6541 cctttcctgt catggccggt tgattctgca gacagatgct cagggggcgt ggtttgagca
     6601 cgatttacat gccctgagcg agcaacagaa ggcggactgt gtggtgctga atccggagaa
     6661 gtcccaccct tacattgagg atatggcgat gtttttatcg ccattaccgg tcgtgctgga
     6721 gtggcattgt gtgatgtgtg agcagttttt tcatgggaag aagttctgtg aggcctgtgg
     6781 tacggggatt tattgcagag cggtatgtac gaagtctgtt tacagctacc agccagactt
     6841 gtttgaggac tgcggaggtg cgagtactta atccgaaatc tccgctctgc gcagggcgtt
     6901 cagaggtatc gcaacctgca ttttatccgg tgtttcgcac ctgccccgtt cgtgacacaa
     6961 aggcgcgaca ccggatatcc gcagtcctgc ttacctgaat aaaacccggg gtattgattg
     7021 gtagtgtggt gcggaatcgt accgtgatct cgcattcgcc gaattcgcac tgtggtgtca
     7081 atcaaccata cgcgagagtc ctctcgtatt tatcagtgcc tcaggacgat ttctgcatag
     7141 ttaatccagg gggctgcatt gttcatttcc ctgctaatgg acgtgacatc ctggatatta
     7201 acgatcctga tttccctgag cagtatcagc gcttattggc acgtctggat tatctgacga
     7261 agctcgacac taaagcatca ggccagcgta actctggcta cagcggtttt tgacctgttg
     7321 taactgtaag gtgatttatt atgtatacag aatcaaatgt taagctttcc tgtaacgccc
     7381 tctggcaaaa gtgtgtcctt gcaactctgt atcgggctgc agctacacat cctgaaatta
     7441 aattattaca atttaaatta ataccagggc agggcattaa taaggccatt ccctctctga
     7501 tggctgattt tactggtgaa ctgcatagac tcatttctga atatttgcag tgcaagaca
//
LOCUS       DAHBHC010000145         7496 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHBHC010000145 DAHBHC010000000
VERSION     DAHBHC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.148"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1908
                     /gene="maeB"
                     /locus_tag="M2A35_004747"
     CDS             <1..1908
                     /gene="maeB"
                     /locus_tag="M2A35_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCB7200938.1"
                     /translation="ALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAI
                     ISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIY
                     QGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMIL
                     ALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEE
                     MKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
                     MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHA
                     TQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQ
                     IMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYR
                     DGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALL
                     SHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPDSPLKG
                     SANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRIVNMVA
                     LAVVEAQTQPL"
     gene            2201..2536
                     /gene="eutS"
                     /locus_tag="M2A35_004748"
     CDS             2201..2536
                     /gene="eutS"
                     /locus_tag="M2A35_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCB7200939.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            2549..3028
                     /gene="eutP"
                     /locus_tag="M2A35_004749"
     CDS             2549..3028
                     /gene="eutP"
                     /locus_tag="M2A35_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCB7200940.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            3003..3704
                     /gene="eutQ"
                     /locus_tag="M2A35_004750"
     CDS             3003..3704
                     /gene="eutQ"
                     /locus_tag="M2A35_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCB7200941.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            3701..4504
                     /gene="eutT"
                     /locus_tag="M2A35_004751"
     CDS             3701..4504
                     /gene="eutT"
                     /locus_tag="M2A35_004751"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCB7200942.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            4501..5517
                     /gene="pta"
                     /locus_tag="M2A35_004752"
     CDS             4501..5517
                     /gene="pta"
                     /locus_tag="M2A35_004752"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCB7200943.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     PLGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            5556..5849
                     /gene="eutM"
                     /locus_tag="M2A35_004753"
     CDS             5556..5849
                     /gene="eutM"
                     /locus_tag="M2A35_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCB7200944.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDVGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            5956..6243
                     /gene="eutN"
                     /locus_tag="M2A35_004754"
     CDS             5956..6243
                     /gene="eutN"
                     /locus_tag="M2A35_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCB7200945.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            6255..>7496
                     /locus_tag="M2A35_004755"
     CDS             6255..>7496
                     /locus_tag="M2A35_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCB7200946.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTS"
BASE COUNT         1752 a         1938 c         2191 g         1615 t
ORIGIN      
        1 gcgctcgaac cgaccttcgg cggaatcaac ctcgaagaca ttaaagcgcc agaatgtttc
       61 tatattgaac agaaactgcg cgagcggatg aatattccgg tattccacga cgatcagcac
      121 ggcacggcaa tcatcagcac tgccgccatc ctcaacggct tgcgcgtggt ggagaaaaac
      181 atctccgacg tgcggatggt ggtttccggc gcgggtgccg cagcaatcgc ctgtatgaac
      241 ctgctggtag cgctgggtct gcaaaaacat aacatcgtgg tttgcgattc aaaaggcgtt
      301 atctatcagg gccgtgagcc aaacatggcg gaaaccaaag ccgcatatgc ggtggtggat
      361 gacggcaaac gtaccctcga tgatgtgatt gaaggcgcgg atattttcct tggatgttcc
      421 ggcccgaaag tgctgaccca ggaaatggtg aagaaaatgg ctcgtgcgcc aatgatcctg
      481 gcgctggcga acccggaacc ggaaattctg ccgccgctgg cgaaagaagt gcgtccggat
      541 gccatcattt gcaccggtcg ttctgactat ccaaaccagg tcaacaacgt cctttgcttc
      601 ccgtttatct tccgtggcgc gctggatgtt ggcgcaacgg ctatcaatga agagatgaaa
      661 ctggcggcgg tacgcgcgat tgcggaactg gcccacgccg aacagagcga agtggtggct
      721 tcagcgtatg gcgatcagga tctgagcttt ggtccggaat acatcattcc aaaaccgttt
      781 gatccgcgct tgatcgttaa gatcgctcct gcggtcgcta aagccgcgat ggagtcgggc
      841 gtggcgacgc gtccgattgc tgatttcgac gtctacatcg acaagctgac tgagttcgtt
      901 tacaaaacca acctgtttat gaagccgatt ttctcccagg ctcgcaaagc gccgaagcgc
      961 gttgttctgc cagaagggga agaggcgcgc gttctgcatg ccactcagga actggtaacg
     1021 ctgggactgg cgaaaccgat ccttatcggt cgtccgaacg tgatcgaaat gcgcattcag
     1081 aaactgggct tgcagatcaa agcgggcgtt gattttgaga tcgtcaataa cgaatccgat
     1141 ccgcgcttta aagagtactg gaccgaatac ttccagatca tgaagcgtcg cggtgtcact
     1201 caggaacagg cgcagcgggc gctgatcagt aacccgacag tgatcggcgc gatcatggtt
     1261 cagcgtgggg aagccgatgc aatgatctgc ggtacggtgg gcgattatca tgaacatttt
     1321 agcgtggtga aaaatgtctt tggttatcgc gatggcgttc acaccgcagg tgcaatgaac
     1381 gcgctgctgc tgccgagtgg taacaccttt attgccgata cctacgtcaa tgacgaacca
     1441 gacgcagaag agctggcgga gatcaccttg atggcggcag aaactgtccg tcgttttggt
     1501 attgagccgc gcgttgctct gttgtcgcac tccaactttg gttcttctga ctgcccgtcg
     1561 tcgagcaaaa tgcgtcaggc gctggaactg gtcagggatc gtgcgccaga tctgatgatt
     1621 gatggtgaaa tgcacggcga tgcagcgctg gtggaagcga ttcgcaacga ccgtatgccg
     1681 gacagccctt tgaaaggttc tgccaatatt ctggtgatgc cgaacatgga agctgcccgc
     1741 attagttaca acttactgcg cgtttccagc tcggaaggtg tgactgtcgg tccggtgctg
     1801 atgggcgtgg cgaaaccggt tcacgtgtta acgccgatcg catcggtgcg tcgtatcgtc
     1861 aacatggtgg cgctggccgt ggttgaagcg caaacccaac cgctgtaatt cttttttaac
     1921 tctcacgctt accctgaata ttcagggtaa gcggtttggc tgcaatatat tagtaaagct
     1981 tattactgag tttgcgaata ataaaaaaag cagtctatat aatatctcga tattatttta
     2041 tttatattca tgcgttgcat atgaaagttt atgcaccaca gcgaatatct ctccattctt
     2101 agtgatctac ctcacctttt aaacgcgctt gccgaatttt gttatttact ctgacgaaaa
     2161 attgtcacga tacacgaaag tttttcacag gcggcgactc atggataaag aacgcatcat
     2221 tcaggaattt gtgcccggca aacaggtcac actggcgcat ctcatcgcgc atcccggtga
     2281 ggaactggcg aagaaaatcg gcgttccgga tgcgggcgca attggcatta tgacgctaac
     2341 tcccggcgaa acggcgatga tcgccggtga tttagcgctg aaagccgctg atgtgcatat
     2401 cggttttctc gaccgcttca gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga
     2461 agaagcgtta tcacaaaccg tcagcggttt aggtcgctta ttaaattata cgctttgtga
     2521 aatgaccaaa agttaatacg aggcattcat gaaacgaatt gcgtttgtgg gtaccgtcgg
     2581 ggcaggaaaa acaacgttat ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac
     2641 acaggccgtg gaatttaatg ataagggcga tattgatacg ccgggtgaat attttagcca
     2701 tccccgctgg tatcacgcct taattaccac gctacaggat gtagacatgc tgatctatgt
     2761 ccatggcgca aatgacccgg aaagtcggtt gcctgccggg ttgctggata ttggcgtcag
     2821 caaacggcaa atcgccgtca tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac
     2881 gcgaaagtta ttgcttgaga cgggtttcga agagccgatt ttcgagctaa acagccacga
     2941 cccgcaaagc gtgcagcaac tggtggatta tctggcatct ctcaccaaac aggaggaagc
     3001 aggtgaaaaa actcatcaca gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg
     3061 caatgtccgt ggttctgcgc gccagcatta ttaccccgga agcccgtgaa gtggcggatc
     3121 tactgggttt taccatcacc gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg
     3181 ccagcgtgcc agccgacaaa accgaaagcc agcgcatccg cgaaaccatc attgcccagc
     3241 tgccggaagg ccagtttacc gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg
     3301 aaaaacagtc gctggagcag ggcgcaatgc agccgagctt caaatcggtg accggcaaag
     3361 gcggcatcaa agtgattgat ggcagcagcg tcaagtttgg tcgtttcgac ggcgcagagc
     3421 cgcattgcgt gggcttgact gatcttgtga ccggagacga cggcagcagc atggccgctg
     3481 gttttatgca gtgggagaac gcattcttcc cgtggaccct gaactacgac gaaatcgaca
     3541 tggtgctgga aggggagctg catgttcgcc acgaaggcga aaccatgatc gccaaagcgg
     3601 gtgacgtgat gtttatcccg aaaggctcca gtatcgaatt tggtacgaca tccagcgtga
     3661 aattcctgta tgtcgcctgg ccggctaact ggcaatccct atgaaagatt tcatcaccga
     3721 agcatggcta agagcgaacc atacgctcag cgaaggcgca gagatccatc tccctgcgga
     3781 cagtcgcctg acgccctctg cccgggagtt actggaaagc cgccatctgc gcatcaagtt
     3841 tattgacgag cagggccgcc tgtttgttga cgatgaacag cagcagccgc agcccgttca
     3901 tgggctgacc agtagcgatg aacatccgca ggcgtgctgc gaactgtgcc gccagccggt
     3961 ggcaaaaaag ccggatacgc tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga
     4021 tccgcgtctg ggttttcgcg cggttctcga cagcaccatt gcgctggcag tgtggctgca
     4081 aattgaactg gcggaaccgt ggcagccgtg gctggcggat atccgttcgc gtcttggcaa
     4141 cattatgcgc gccgatgcgc tgggtgaacc gctgggctgc caggcgattg tcgggctttc
     4201 tgacgaagat ctgcatcgac tttctcacca gccgctgcgc tatctcgacc acgatcatct
     4261 ggtgccggaa gccagccacg ggcgcgatgc ggcgttgctc aatctgctac gtaccaaagt
     4321 gcgggaaacc gaaaccgtag cggcgcaggt gtttatcacc cgcagctttg aagtattacg
     4381 cccggacatt ttgcaggcgc tgaaccgcct ttccagcacg gtctacgtga tgatgatcct
     4441 gagcgtaacg aagcagccgc taacggtgaa gcagatccaa caacgactgg gggaaacgca
     4501 atgattattg aacgttgtcg tgaactggcg ttgcgagcgc ccgccagagt ggtttttccg
     4561 gatgcgttag accaacgtgt gctgaaagcc gcgcaatatt tacatcaaca aggtctggca
     4621 acgcccattc tggtcgccaa tccgtttgaa cttcgtcagt ttgcgctcag tcacggcgtg
     4681 gcgatggacg ggctacaggt gattgatccg catggcaacc tcgcaatgcg ggaagaattt
     4741 gctcatcgct ggctggcccg cgcgggcgaa aaaacgccgc cggatgcgct ggaaaaactt
     4801 accgaccctc tgatgttcgc cgcagcaatg gtcagcgccg gtaaagcgga tgtctgtatc
     4861 gcgggcaacc tctcttccac ggcaaatgtg ctgcgtgccg gattacgcat tatcggcttg
     4921 cagccaggct gtaaaacgct ctcttccatt ttcctgatgt tgccacagta cagcggcccg
     4981 ccgttgggct ttgccgattg cagcgtggtg ccacagccga cggcggcgca gctggcggat
     5041 atcgcgcttg ccagtgccga aacctggcgc gccatcaccg gagaagagcc gcgcgtggcg
     5101 atgctgtcgt tttccagcaa tggtagcgcc cgtcacccct gtgttgctaa cgtccagcag
     5161 gcgacagaaa tcgtccgtga gcgcgcacca aagctggtgg tggatggcga gttacagttt
     5221 gacgccgcct tcgtgccgga agtggcggcg caaaaagcgc ctgccagccc gctacagggc
     5281 aaggccaatg tgatggtttt tccgtcgctg gaagctggaa atattggtta caaaatcgca
     5341 caacgactcg gcggatatcg tgccgtcggg ccactgatac aaggacttgc cgcgccgatg
     5401 cacgatctct ctcgtggttg tagcgtgcag gaaattatcg agctggcgct ggtggcagct
     5461 gtgccgcgtc agacagaagt gaaccgcgaa agcagtttac aaacactggt tgaatgaacg
     5521 gtcccgttct ggaccctgat aaagaggaaa gcacaatgga agcattagga atgatcgaaa
     5581 cccggggcct ggttgcactg attgaggctt ctgacgcgat ggtgaaagct gcgcgcgtca
     5641 agctggttgg tgttaagcag attggcggcg gcctgtgtac tgccatggtt cgtggtgatg
     5701 ttgcagcctg taaagccgcg accgatgtag gtgccgcagc ggcacagcgt atcggcgagc
     5761 tggtatctgt acacgttatc ccgcgtccgc acggcgacct ggaagaagtc ttcccgatcg
     5821 gcctgaaagg cgatagcagc aacctgtaat caccctgtgt gacgtcgatt cgggcgtcac
     5881 gctctctcct ttttcatttt accttctgcg gagggtaggg ggagctttta cctgaaatac
     5941 gccacggagg cgggtatgaa actggcagtc gtcactggac aaattgtttg taccgtacgc
     6001 catcacggac tggcgcatga caaattgctg atggtggaaa tgattgatcc acaaggtaat
     6061 cctgacgggc aatgcgccgt cgccatcgac aatattggcg cgggaaccgg ggagtgggtg
     6121 ttactggtga gtggcagttc cgcccgccag gcgcataaaa gcgaaacgtc accggtcgat
     6181 ctgtgcgtga ttggcattgt cgatgaggtg gtgtctggcg gtcaggtaat tttccacaaa
     6241 taaggcagaa catcatgaat caacaggata ttgaacaggt ggtgaaagcg gtactgctga
     6301 aaatgcaaag cagtgacacg ccgtccgccg ccgttcatga gatgggcgtt ttcgcgtccc
     6361 tggatgacgc cgttgcggca gccaaagtcg cccagcaagg gttaaaaagc gtggcaatgc
     6421 gccagttagc cattgctgcc attcgtgagg caggcgaaaa acacgccaga gatttagcgg
     6481 aacttgccgt cagtgaaacc ggcatgggcc gcgttgaaga taaatttgcc aaaaacgtcg
     6541 ctcaggcgcg cggcacgcca ggcgttgagt gtctctctcc gcaggtgctg accggcgaca
     6601 acggcctgac tttaattgaa aacgcgccct ggggcgtggt ggcttcggta actccgtcca
     6661 ctaacccggc ggcaaccgta attaataacg ccatcagcct gattgccgcg ggcaacagcg
     6721 tcatttttgc cccgcatccg gcggcgaaaa aagtctccca gcgggcgatt acgctgctca
     6781 accaggcgat tgttgccgca ggtgggccgg aaaacttact ggttactgtg gcaaatccgg
     6841 atatcgaaac cgcgcaacgc ttgttcaagt ttccgggtat cggcctgctg gtggtaaccg
     6901 gcggcgaagc ggtagtagaa gcggcgcgta aacacaccaa taaacgtctg attgccgcag
     6961 gcgctggcaa cccgccggta gtggtggatg aaaccgctga cctcgcccgt gctgctcagt
     7021 ccatcgtcaa aggcgcgtct ttcgataaca acatcatttg tgccgacgaa aaggtactga
     7081 ttgttgttga tagcgtagcc gatgaactga tgcgtctgat ggaaggccag cacgcggtga
     7141 aactgaccgc agaacaggcg cagcagctgc aaccggtgtt gctgaaaaat atcgacgagc
     7201 gcggaaaagg caccgtcagc cgtgactggg ttggtcgcga tgcggcgaaa atcgcggcgg
     7261 caatcggcct gaacgtgccg caagaaacgc gtttgctgtt tgtggaaacc actgcagaac
     7321 atccgtttgc cgtgaccgaa ctgatgatgc cggtgctgcc cgtcgtgcgc gtcgccaacg
     7381 tggcggatgc cattgcgctg gcggtgaaac tggaaggcgg ttgccaccac acggcggcaa
     7441 tgcactcgcg caacatcgaa aacatgaacc agatggcgaa tgctattgat accagc
//
LOCUS       DAHBHC010000146         7322 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHBHC010000146 DAHBHC010000000
VERSION     DAHBHC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.149"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(39..862)
                     /locus_tag="M2A35_004756"
                     /pseudo
     CDS             complement(39..862)
                     /locus_tag="M2A35_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(916..2634)
                     /gene="proS"
                     /locus_tag="M2A35_004757"
     CDS             complement(916..2634)
                     /gene="proS"
                     /locus_tag="M2A35_004757"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCB7200947.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2746..3453)
                     /gene="tsaA"
                     /locus_tag="M2A35_004758"
     CDS             complement(2746..3453)
                     /gene="tsaA"
                     /locus_tag="M2A35_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCB7200948.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3450..3854)
                     /gene="rcsF"
                     /locus_tag="M2A35_004759"
     CDS             complement(3450..3854)
                     /gene="rcsF"
                     /locus_tag="M2A35_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCB7200949.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3972..4787)
                     /gene="metQ"
                     /locus_tag="M2A35_004760"
     CDS             complement(3972..4787)
                     /gene="metQ"
                     /locus_tag="M2A35_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCB7200950.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4827..5480)
                     /gene="metI"
                     /locus_tag="M2A35_004761"
     CDS             complement(4827..5480)
                     /gene="metI"
                     /locus_tag="M2A35_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCB7200951.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5473..6504)
                     /gene="metN"
                     /locus_tag="M2A35_004762"
     CDS             complement(5473..6504)
                     /gene="metN"
                     /locus_tag="M2A35_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCB7200952.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6692..7264
                     /gene="gmhB"
                     /locus_tag="M2A35_004763"
     CDS             6692..7264
                     /gene="gmhB"
                     /locus_tag="M2A35_004763"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCB7200953.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1799 a         1898 c         1857 g         1768 t
ORIGIN      
        1 acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag cccaacaaag
       61 cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa ctgcgtttct
      121 ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag
      181 cccgattgcg ccagtgtaag tggatgcccg gctttggcaa aggcatccgt gacaaattcc
      241 gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc tttcgccagg
      301 ccgctgacgc actttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
      361 tcaccataaa gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt
      421 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
      481 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
      541 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
      601 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
      661 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
      721 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
      781 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga aacaggcggc
      841 agtacgcctt tggtttatcc attttataca atccatgtaa aaaaagggcc ctgaaattca
      901 ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt
      961 cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag
     1021 gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat
     1081 cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc
     1141 gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag
     1201 aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgcctcgtt cgtcgtagtt
     1261 ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag
     1321 gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt
     1381 acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc
     1441 atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac
     1501 atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg atgttagcac cagcagcgaa
     1561 atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc
     1621 cagtgaaccc ggaccggctt taaccacggc acgaatttct tcttcggtcg cgaaagtcag
     1681 cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg
     1741 caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca gcagagtctt
     1801 aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt
     1861 cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat
     1921 agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc
     1981 ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt
     2041 gtcggcttgt acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc
     2101 atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc
     2161 tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa
     2221 cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg
     2281 aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg
     2341 gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa
     2401 atcagctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc
     2461 acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa
     2521 cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc
     2581 ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt
     2641 acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaaagtgatt
     2701 tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag
     2761 cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca
     2821 gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgtg ggtcctgcgc
     2881 cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg
     2941 cttttcgacc tctgcggtaa aactcaccgc catctctgcc gctggcgcac tttgcgcata
     3001 gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac
     3061 cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca
     3121 aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgga aagtagagcg
     3181 tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg
     3241 ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaacgcttc
     3301 caggccgcga acggcgtcgg cctggttgta gggagcaatg agatgcagtt cgccgttggc
     3361 gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat
     3421 aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg
     3481 atacatacag cctgacgata gcagcctggc gtaccgctgg tgacttcgca gctatgcagt
     3541 aataccgcat tggccttcat tttcgaggcg ttgatttgca tccgcttacg cgcggttgga
     3601 atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga
     3661 tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg
     3721 cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt
     3781 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
     3841 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
     3901 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
     3961 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
     4021 taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
     4081 tcttcacgcg ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg
     4141 tctttagctg gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg
     4201 atttgcgcgt cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac aattttcaga
     4261 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
     4321 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt
     4381 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
     4441 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
     4501 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
     4561 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
     4621 ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg
     4681 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
     4741 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
     4801 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
     4861 cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
     4921 gtcgcgttat agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
     4981 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
     5041 accagacccg gcaatgcttc cggtaacagc actttacgga cgatctgcat cggcgtggca
     5101 cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
     5161 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caatcgctgc ctgcaaacca
     5221 atcgatgtac caacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
     5281 gggatagaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
     5341 ataatttgcc ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaag
     5401 ccaaaaaaac cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc
     5461 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
     5521 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
     5581 cttaacgcca ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
     5641 acgcgcggtt tcagaaagca gtggggcatc gaccgactga ccggtaaact ccagacgcag
     5701 catcggcacg cagtcagtga atggctccgc ttgcagacgt tcctggtaat cttccgggat
     5761 atccagatgc agggtcgact gaataaactt ctgcgccaac ggcgttttcg gatgcgaaaa
     5821 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc
     5881 acaaatacgc tttacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
     5941 gcggttaatg tctttcagca gctcgagaat agaacgtgtc gttgccgggt ccagcgcgct
     6001 ggtggcttca tcacacagca atactttggg attgctggct aacgcacggg caatcgccac
     6061 acgctgtttc tgcccaccgg aaagattcga tgggtagcta tcatgcttat cgccaagacc
     6121 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag
     6181 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
     6241 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag
     6301 ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg
     6361 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
     6421 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
     6481 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca
     6541 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
     6601 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
     6661 cgtcacatgc cttattaagg agctataaaa ggtggcaaag agcgtacccg caatttttct
     6721 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
     6781 atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt
     6841 ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
     6901 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
     6961 ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgactgccg
     7021 taaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
     7081 ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggctgcgg cgaacgtggg
     7141 aacaaaagtg ctggtgcgta cgggtaaacc tattacgcct gaagcagaaa acgcggcgga
     7201 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
     7261 ataatgatta aaagatgagc aattgaaata aaaatgcatt tttccacttg tcttcctgag
     7321 ac
//
LOCUS       DAHBHC010000147         7316 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHBHC010000147 DAHBHC010000000
VERSION     DAHBHC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.150"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            403..1521
                     /locus_tag="M2A35_004764"
     CDS             403..1521
                     /locus_tag="M2A35_004764"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCB7200954.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1587..1835
                     /locus_tag="M2A35_004765"
     CDS             1587..1835
                     /locus_tag="M2A35_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCB7200955.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1900..2268
                     /locus_tag="M2A35_004766"
     CDS             1900..2268
                     /locus_tag="M2A35_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCB7200956.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFSPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALIRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2362..3015
                     /gene="nfsB"
                     /locus_tag="M2A35_004767"
                     /pseudo
     CDS             2362..3015
                     /gene="nfsB"
                     /locus_tag="M2A35_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            3123..4370
                     /gene="ybdG"
                     /locus_tag="M2A35_004768"
     CDS             3123..4370
                     /gene="ybdG"
                     /locus_tag="M2A35_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCB7200957.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4438..5814)
                     /gene="pheP"
                     /locus_tag="M2A35_004769"
     CDS             complement(4438..5814)
                     /gene="pheP"
                     /locus_tag="M2A35_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCB7200958.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5916..>7316)
                     /gene="cusA"
                     /locus_tag="M2A35_004770"
     CDS             complement(5916..>7316)
                     /gene="cusA"
                     /locus_tag="M2A35_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCB7200959.1"
                     /translation="AAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVE
                     TTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGI
                     KVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVA
                     DVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLA
                     DVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVA
                     FSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLL
                     WWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEAL
                     YHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFII
                     PAAYKLMWLHRHRVRK"
BASE COUNT         1792 a         1965 c         1779 g         1780 t
ORIGIN      
        1 aattgttcag gtttgggtag gtagccccag ggttgataga aatatcgtcc tggggcttca
       61 tctttccaga cctcagagta aacctgaaac cggaaagctt caggcacccg cagttatctt
      121 acccttgctt agcaaaatag ctgtttattt atacagtaag tcctgtttcg gacttctcac
      181 tccccatttg atcaactgaa gctgaacccg tttttttctg catgcctcaa tcgtcaaaat
      241 tcttcgccac cagccagccg tgccataact tgtcacattg caaaagtagc caaattgctt
      301 cagtcaccat caccaaaaaa agaaacattc cagaacttcc gcatctcgcc cagccgctcc
      361 gacttctata ctgaatagaa aacgccaact taagagaaac ctatgccatt acccgatttt
      421 catgtttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt taatccgccg
      481 ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa tgagatcacg
      541 gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac ggatgtttgc
      601 cgtgatatca accaggctgc cgggcagttt tcagcgatgc agaaagtcgt attgcaggca
      661 gccgcagacc atcatctgga aatttgcggc ggtggcacgc acccgtttca gaaatggcag
      721 cgtcaggagg tatgcgacaa cgaacgctat caacgaacgc tggaaaactt tggctatctc
      781 atccagcagg cgaccgtttt tggtcagcat gtccatgttg gctgcgccag tggcgatgac
      841 gccatttatt tgctgcacgg cctgtcacgg tttgtgccgc acttcatcgc cctttccgcc
      901 gcgtcgccgt atatgcaggg aacggatacg cgttttgcct cctcacgacc gaatattttt
      961 tccgcctttc ccgataatgg cccgatgccg tgggtcagta actggcaaca atttgaagcc
     1021 ctgtttcgct gcctgagtta caccacgatg atcgacagca ttaaagatct gcactgggat
     1081 attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac cccgttaacc
     1141 cttagccacg cggtaaatat ggcgggatta attcaggcca ccgcccactg gttactgacg
     1201 gaacgcccgt ttaaacatca ggagaaagat tacctgctgt ataaattcaa ccgtttccag
     1261 gcctgccgct atgggctgga aggcgtcatt accgatccgc acactggcga tcgtcgaccg
     1321 ctaacggaag ataccttgcg attgctggaa aaaatcgccc cttccgcaca taaaatgggt
     1381 gcatcgagcg cgattgaggc cctgcatcgc caggtcgtca gcggtctgaa tgaagcgcag
     1441 ctgatgcgcg atttcgtcgc cgatggtggc tcgctgattg ggctggtgaa aaagcattgt
     1501 gagatctggg ccggtgacta agtagggaat tgccttacag aagaatatcc ctgacaatgg
     1561 tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg actaccgcag
     1621 caggcatttt gctgatggct ttcctctctt gcctgctgct gcccgccccc gcactggggc
     1681 tgacgctggc acaaaaactg gtgaccatgt tccatctgat ggatcttagc cagctttaca
     1741 ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt gttctgcgct
     1801 ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg
     1861 gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac
     1921 gaaacggcga aacgcctggc ccttgagtta ccctttgtcg agctttgctg gccttttagc
     1981 ccggagttcg atgtttttaa aattggcggc aaaattttta tgctgtcgtc ggagctgcgc
     2041 ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcagata
     2101 tacccgagca ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtatccc
     2161 ggcgaggaaa tctccgaagc gttaattcgc gatctgatca acgattcgtg gaatctggtg
     2221 gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta
     2281 tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa
     2341 ttactttcac atggagtctt tatggatatc atttctgtcg ccttaaagcg tcattccact
     2401 aaggcatttg atgccagtaa aaaacttacc ccggaacagg ccgagcagat caaaactctc
     2461 ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt tgccagcacg
     2521 gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt caacgaacgt
     2581 aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc
     2641 tggctgaagc tggttgttga ccaggaagat gctgatggcc gctttgccac gccggaagcg
     2701 aaagccgcga acgataaagg tcgcaagttc ttcgctgata tgcaccgtaa agatctgcat
     2761 gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaactt cctgctcggc
     2821 gtggcggcgc tgggtctgga cgcggtaccc atcgaaggtt ttgacgccgc catcctcgat
     2881 gcagaatttg gtctgaaaga gaaaggctaa accagtctgg tggttgttcc ggtaggtcat
     2941 cacagcgttg aagattttaa cgctacgctg ccgaaatctc gtctgccgca aaacatcacc
     3001 ttaaccgaag tgtaattctc tcttgccggg catctgcccg gctatttccc ctcagattct
     3061 cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat aaaggagacg
     3121 ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc gatcacacca
     3181 cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg catattattt
     3241 tgcattgggt ggtactgcgg accttcgaaa aacgtgccat cgccagttca cggctttggt
     3301 tgcaaatcat tacccagaat aaactcttcc accgtttagc ttttaccctg caggggatta
     3361 tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca gatattctga
     3421 ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg
     3481 atgttatttt gaatctggcg cagaaattcc cggcggcatc tcagttaccg ctgaaaggga
     3541 tatttcaggg gattaaactg atcggcgcaa tcctggtcgg cattttgatg atctcgctgc
     3601 tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct gccgtgctga
     3661 tgttggtttt taaagatccg attcttggtc tggtggcagg tattcagctt tccgcgaacg
     3721 atatgctgaa actgggcgac tggctggaga tgccgaaata cggcgcggat ggcgcggtga
     3781 tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt accactattc
     3841 ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca gcatctggcg
     3901 ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt cttgatgaag
     3961 acgaaatgca acgtctgaat aaagcgcact tgttaaaacc ttatttaacc agccgccatc
     4021 aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcgatatta aacctgcgcc
     4081 gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt aaccatccgc
     4141 ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat aacggtttac
     4201 cgctcgagat ctacgcgttt actaacaccg tggtgtggct ggaatatgaa agcattcagg
     4261 ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga cttcatcagt
     4321 cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac
     4381 cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt gaatgcctta
     4441 tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg gcagcaggat
     4501 cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc
     4561 aatgcaaaga tagttgccga acggatagag cagcgcctta aactgtgttt cacgcccctg
     4621 acgtcgcatc gctgcacgaa aacgcagatg cgccagacag atcataatcc agttcaacag
     4681 cagcgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt
     4741 gattaatacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc
     4801 gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag
     4861 catgcggctg ttagagtaaa ccccgctgtt atacactgac agcgatgcta ccagaatgac
     4921 gaagttcagc gcagaagcta ccacgttgct gtcgagatta tggaaaatca tcacaaacgg
     4981 gctactgttg gatttcactt ccacccacgg atagagcgcc agtaaaacca ccagtgaacc
     5041 gatgtaaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa tgcttttttc
     5101 cggatcgcgc gcttcagcgg cagtaatccc aatcagctcc agaccgccga aggagaacat
     5161 aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac caccgtagcg
     5221 ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca gccacaggcc
     5281 aaagccgatc ataccgatga tcgccagcac tttaatcagc gcaaaccaga actcggtttc
     5341 accatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc
     5401 ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag
     5461 ctctgccatt cccaccagca cgaacattac ccagtagttc cagccagaga ggaagcccgc
     5521 aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg gctcctcaac
     5581 caccatttcg ccaagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc
     5641 cagcaataca gccggacccg ccatctgaat cgccgggcca atgccaagaa acagaccagt
     5701 accaattgcg ccacccaacg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag
     5761 cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt tcacgccttt
     5821 cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag
     5881 acacaatcca cacggttaaa cggggtatcc tgcttttatt tccgtacccg atgtcggtgc
     5941 agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg tgcggtgatc
     6001 atgccgccaa tcatcggtgc ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc
     6061 cacagaatcg gcagcagacc ggcgataatc accgccaccg tcatcgcttt cgggcgcacg
     6121 cgcaggaccg cgccgtgata taacgcctca tccagcttct gctcgctgaa tgtttgtgga
     6181 ttattcaacg acggcacggc ctctatggcg tgacgtaaat acatcagcat caccacgcca
     6241 aattcggcgg cgaccccggc gagggcgata aagccagtgc ccgtcgccac ggaaagatga
     6301 aagcccatcc accacagcaa ccagatgccg cccaccagcg caaacggtac gctgctgata
     6361 atcagcaacg cttcgcccac ccgacggaac gccagataca acagcacgaa gataatcatc
     6421 aacgtcatcg gcaccatgag tttaagctta tggttggcgc gctccagtag ctcgaactgc
     6481 ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc
     6541 aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc
     6601 gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaat gtcggccacg
     6661 tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcagctggcg cagtgcctgc
     6721 ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc aatcccttcc
     6781 accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc
     6841 accgtcatac cgtaacgtgc ggctttttca cggttaatct caacgttgat atagcgccca
     6901 ccttccagcc gctcggcaag cgcagaagct acgcctggca ccgttcgcgc tacttcttca
     6961 atttgctcag ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt aatgccgatg
     7021 gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg cacccacaga
     7081 ttcgccagcc ccggcagccg cacggtgtta tccagttcct caatgatttt gtccatcgtc
     7141 atgcctggcc gccactgctc ctgcggctta agctggatgg tcgtttctac catctccagc
     7201 ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttca
     7261 ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc tgctgc
//
LOCUS       DAHBHC010000148         7316 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHBHC010000148 DAHBHC010000000
VERSION     DAHBHC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.151"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(233..2083)
                     /locus_tag="M2A35_004771"
     CDS             complement(233..2083)
                     /locus_tag="M2A35_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCB7200960.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWVCGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAAGRTSAFISGKA
                     PEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGGIQLSDGVFKITKQS
                     NKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLMLEINAPAA"
     gene            2060..2301
                     /locus_tag="M2A35_004772"
                     /pseudo
     CDS             2060..2301
                     /locus_tag="M2A35_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2382..2540)
                     /locus_tag="M2A35_004773"
     CDS             complement(2382..2540)
                     /locus_tag="M2A35_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCB7200961.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(2626..3369)
                     /locus_tag="M2A35_004774"
     CDS             complement(2626..3369)
                     /locus_tag="M2A35_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200962.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(3554..4243)
                     /locus_tag="M2A35_004775"
     CDS             complement(3554..4243)
                     /locus_tag="M2A35_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7200963.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(4258..4380)
                     /locus_tag="M2A35_004776"
     CDS             complement(4258..4380)
                     /locus_tag="M2A35_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCB7200964.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            4719..5678
                     /locus_tag="M2A35_004777"
     CDS             4719..5678
                     /locus_tag="M2A35_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HCB7200965.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(5822..5893)
                     /locus_tag="M2A35_004778"
                     /pseudo
     CDS             complement(5822..5893)
                     /locus_tag="M2A35_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
     gene            complement(5890..6555)
                     /locus_tag="M2A35_004779"
     CDS             complement(5890..6555)
                     /locus_tag="M2A35_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCB7200966.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(6552..7163)
                     /locus_tag="M2A35_004780"
     CDS             complement(6552..7163)
                     /locus_tag="M2A35_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCB7200967.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(7156..>7316)
                     /locus_tag="M2A35_004781"
     CDS             complement(7156..>7316)
                     /locus_tag="M2A35_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCB7200968.1"
                     /translation="NRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSDSNSS
                     MHEEEDDG"
BASE COUNT         2045 a         1811 c         1490 g         1970 t
ORIGIN      
        1 gccatataga agcaacatgt gaagtacatc tatccttttg agccagcacc tctccaccga
       61 aagtcagtgc tggctgtttt tttccttaat aaggcatctg taactgaaac aatccgcata
      121 ttgataatat attgacaggc atcattgctg tctgtgaaaa ataagtctct acaaacatat
      181 aaggcctttt agccagcgtc ttctttttca ggtcagtcgc tggctttttt tattatgctg
      241 ccggtgcatt tatctccagc atcagacttt ctatctcaac gccatacgct gcattttttg
      301 taacatccgt cagcgtcagc gcattcagtc ccagtgtcag actgtctttt ataacctgga
      361 atgccgggcc agccactcca ttcagtttcg gagtaaccgt ggcactgccg gcggtgaaca
      421 ccagctccag cgtctgccag tcgttaccgt aatcgccgaa ctcccccagc ttcgtgtttc
      481 cggctttcct gtgatgcatc agattcactc tgccgtcagt ggtctgagtg aagtacgaca
      541 tcaggaacgg attaccggta cccgtcatcg ccacaccatc aggaacggga gcatccgtat
      601 acagataaat ccccagcccg aactgattgt tggtcagtgc gcctgacagg cggaacttac
      661 aggtcagtct gccgccctgt gtcagcaggg taattgcgtc atccaccgga tgcgtcaggg
      721 accaggtttt attgctctgc ttggtgatct taaatacacc atctgacaac tgaattccgc
      781 catccttaat gctccagccc tgcgcagcag cctctccggc tgccggcagc agggagattg
      841 tgcgaacgga cgtatctgca gacggacccg atggcgtgtt gccgccgggc gagggtttga
      901 tttccggtgc cttaccactg atgaaggctg aggtgcgccc ggctgcgttc agaatagcgg
      961 ttgccagacg atccggaata atgctcctgc gcgcccatga actgaaatgt gtcgggcggt
     1021 ttgatgatac ctggtttcca ttcgttctcg atgccgcacc gtaatatcct gatgccggaa
     1081 tatccggatc ttctgccggc gcgttagtgg cggtattgac gccgttacca tctgtcatga
     1141 agggcacaaa ataaacgccc tcactctccc tgtttttata cccgccgtac acggtgtcgt
     1201 actgggtagc gtatgtattt ttccagtaat acgtcgtgtc accacaaacc cacggcacat
     1261 ctgcagcact gccaccatgg cactgcgcgt taaacacgga gaggtcagca cgaaactgtg
     1321 tcagcatggc tgtaaacagc gcaggttgct gtgcgtgggt ggcggcgctc atgtcaaact
     1381 caccctgcat ccagcagacg gccagcagaa cgtttttggg atttttctgc aatgccgctt
     1441 ttgtgcggga aatcagatcc tgatataacg gcttgcccac cccccagcgt gccgaatcct
     1501 gactggcccc cgtggactcg ctgaatgtcc cctccgcgcc ctgggtaaat gccgaaccac
     1561 cacgacagca tggtaccagc aggatccccg cgttattcgg gatatacggg agcagttttt
     1621 tggcaatatg taaaccctga ccgacacagc cgtactgccc tttgctcagg tcagccctcg
     1681 gatgattcag cgtactcata tcctgcacat catgcagaca gtggtcagcc ggaataatat
     1741 cgttatatct gcaggcagcc ccgcccggcg tcactgtact gcggcgcgcc agctgtttaa
     1801 tgcgcggatc cggagcatcg tatgaatccg gcagcggaag cccttcaccg taagccatgg
     1861 cattagactg cccggccagt acgatgacgt agtaccaatc cggctcagat gaagggccga
     1921 cctgtggctc tccttcaata gccaccgcct gcatcagtgt gtacggcgta atggcaaccg
     1981 gtccgccgta tggctgccag ccctctttca gtttgtgtgt cagcttttcc gcaaggtctg
     2041 acggcgacgc cgccctgaca acatcgtaat gtttaatcga catcgaattt ctcccgtgta
     2101 gaggaacaga gttaaaaagc cggaagcgga atcaaatcac aggatgacca tctgccagtg
     2161 gcaggtcata aaaaaaaggc tgcgcaatgc gcagccagaa ctcacaagga aaatgataaa
     2221 aggaataaca ctagtgatgt acgcatggcg cctcccgcta agttctgcaa tgatcaaaca
     2281 gaactcgcta cgtgccctta aaactcgatc atttagcccc tccaaggagg attcaccatg
     2341 cggttgattt tttaataaac agtaaacaaa aaagtcaaga attattcatt ctgttctttc
     2401 atcatcggcc acagcaatac cacaatgccg cagaccagag cgccatcagt cagtaccaac
     2461 attatcctgc tggtgaaatc catcatcacc atcactaaaa gcaggatcac aacagcaagc
     2521 agacacagtt tataaaacaa tgttcagaaa acgcattcag catgcctaag gttctattcc
     2581 tacgaatagc caacttgcaa cttaaaatat tatttatgca gccaattaaa ttctggtcct
     2641 tacaatatca acctgaagat tcttatcttg tgctgattga taaatgacaa accttttact
     2701 acctgcattg aaagaagtag acaaaaccag acaattatca taacgagcaa gaacataata
     2761 ccaaccatca ttataattaa tcatttcata ttctttctta aactgtggtt tgtaatatcc
     2821 tgtcagaaat gaaaaaagcc aaaaatatgc cacaaaagca atcatcacaa tctcaaaaaa
     2881 atgttttttt ataaatggct tatcatagaa gcatgatacc gataaaaatc gcccataaga
     2941 tcttatcgaa attgtaaccg ccagcgcaat cgctgctgac agtagcaaaa gaggtacctg
     3001 aatcttctgt ctcaatatag aaaactcaat aattgccggc acaaacaata attccacagc
     3061 aaaataaagg cgaaatacat ttagctcttg catagaatgt tttcttttca ctgcgaaaaa
     3121 gaatacaaca ccaatacccc aaccgataag aaatatagca atgacgataa ctgcaaaaaa
     3181 taaacttctg gcaacatcat caacacctgc acctacaatc caccatggga agccgtagta
     3241 aaaagaagta ccccatccat agaaataagc actcccccat ccaaggcatc ccatgtaggc
     3301 aataaaaagt gaagaactcc tgagcagcgc accatccttc ataaccaccc caatacaaga
     3361 tgataacatt ggcttacaac tcataacaaa agcaattcaa tgccgtcaag aggttacagg
     3421 ctaaaaaaac tctattacat agcagccagc atgtttaccg tacaagtaca actcagggca
     3481 taaaaaaaac ccactcggca gcgggtttat acatttttta caacatacca aatttgcatg
     3541 aagtatatgg cttttaatcc agttttgcaa tattttgctg taaaaatgct gccttttgtt
     3601 ttgaacgtgt tctcgtcaca agcaataaag catcactatc aagctgtaga aaaatgtgct
     3661 tcattgcaac ccagcgttca gtaaatgtct cggaccagtt ttttgttgtc actcccacca
     3721 atgatgccag tgtctggtat tcataggcct cacgccctgc aagttcgctc ttcacatcct
     3781 gtgcagccag ccagattaac gtcttcaggc gatccagtgt cttacctgca atttttctgt
     3841 tacttaacaa atctttaaac tcgctccatg cccattgcgt tatggtgacc tgatgccccc
     3901 atcgaacgct ttcgctgtaa caccaaagca accatgcttt ctgatgttca tcgagagaca
     3961 aaaccgcgcg gcgccatgaa gaggttgaga attcaaccgg gctgaccaaa gcaatggatg
     4021 aaccttttgc gtacgactgc ttaccggaag tcggcgtatt atccagcgta atcatcttgc
     4081 cagttaccac atccagaatg cgcggcttct ttcgtttgta tgtaccagta tcaaattgtg
     4141 catgctcctg ccaggcttcg agctggcctt tcgttgctcc gttcaagtca gcagtagctg
     4201 ccataagttg ctcacgaaca tactgtaaat attgggtatt catgcagtaa atcctttcta
     4261 tattttggca taattcttca acattcggta atcgttcaaa accgaatcgg ggaaacgaca
     4321 taagcacagg agcccccagc gacagcgaag gagttctgat atataagact cagacatcat
     4381 tcattccccg gttctccaat atctgtttca ctcatcatcc ataactacct gtaattgccc
     4441 cccctttttt tgtaacagtt cttatattgc tatatagaat agccattact aatgcattta
     4501 aatttaataa aacaaaaatt ataaaaaaca taaaacacca cgcaaacaca cttaataaaa
     4561 acaccgttac attaaaagat aataaaaacc gcaataaaaa acgaataaat caattgtctc
     4621 acgcaattat aaaacatcat attgattacg caccttgtat tacaaactca tgtatgtaaa
     4681 atacgcgcac cattcaaaaa aaaggaagac aataacatat gaaaaaaagt gtcatcgctg
     4741 gcgtctttat tgctctgtca tttaccacgt gttcagctat cgcgaacagc cttgcattat
     4801 cattagcaaa tgatgatgca gggaagtttc aaccaatact taatgatatt tatggcaata
     4861 aacatgaaaa cagagatgat tactcacaag gcttatttct gggatatagc cacgatatct
     4921 cagactcgag ccaattatct ctccatattg cgcaagatat ttactctcca tcaggcagta
     4981 ataaaagaca caacacagct gtaactggag acagagcttt tagtgcatac actcacactg
     5041 gtattgaatg gaactccctt gcgaatgact ggattcgcta tcgattaggt actgacatag
     5101 gtgttgttgg ccccgacgca ggcggtcaga aagtacaaaa taaagctcat gagattattg
     5161 gggcagaaaa atatcatgca tgggatgatc aaatagagaa tcgctacggt tatactgtaa
     5221 aagggatgct atccatgaca ccaagtatgg atattttagg tgctaatgtt ggattatacc
     5281 ctgaagtttc tgctgttact ggaaacttat ttcaatatgt agcatatggc gcaaccattg
     5341 ccattggtaa tgataaaacc ttcaattcgg ataatggctt tggtctgctg gctccccgtg
     5401 gtttaatgca tatgtccgat acaagcggat tcaaatacaa gatttttgca ggtatggaaa
     5461 gacgagatgt caatcgcaac tatactctcg aaggaaaaac aatacagacg aaacaaacaa
     5521 cagtatcgct aaacaaaact gttgatgaat atcaagttgg cgcaacaatt gggtatgcac
     5581 ctgtagcctt cacactagca tttaataaag taacatcaga attcaagaca ggggatgact
     5641 attcatttat aaatggagca atcaccttct ttttttaact gaattgaatt caatcaaaat
     5701 aacataagtc caacaaaaac ataaagtgcg aaatgaatgc cagctccatt tatttcgcac
     5761 tataaaagat taaaagttgc aataaaataa taaaatgact cagttacgaa aaccaataaa
     5821 ctgtggccag tagtgagtcg ctcatcatcg ggctttttgg cgaatgaaat ttagctacgc
     5881 tttcgagtct catgcgcctt ctccctgtac ctgaatcaat gtgaggtttc cgcagaacac
     5941 tgcgccagta tcgatataca tctggttggc aaatttgagt ggtttcactg ctggcgtatg
     6001 accaaagatg aacgtgtccg cgcctttgat ttctttcacg atcccgtctt gtgagttgct
     6061 gattcgttcg cggttccaga ttacctgctg atgatcaact ggctttccaa actcgtattt
     6121 atcacaagga taatcggcgt ggcaaatgac atatttttta tctttgctca ccagttcgat
     6181 gattaacgga agttcttctg ctttatgggc aagaacttta gccagagtct ctttgtcgta
     6241 atcgagatta aagaaccagc caccgccatt aagcagccag tgattaacgt ttccacgctc
     6301 tgataagcca tcaatcatca tttgctcatg gtttccacgc acagctctga accaggggaa
     6361 tgtgattaat tccagacatt cgacgttctc tgtaccgcga tcaaccaaat cgccaaccga
     6421 gatgagcagg tcttttttgg tgtcgaatcc aatcgtatcc agtttgttca tcaggttcgt
     6481 gtagcatccg tgcaggtcgc cagctaccca aatatttcgg tatttgctgc catcaattct
     6541 ttcgtagata ttcatgcagc ctcacttctg ctgtttcgca ggtctttgag tttctgttgg
     6601 tactctgcct tgatcgcctt gcactcttcg acagtccagc gatggcggtt atggtttgat
     6661 tcgatttcgt ctactgcttc ctgcccgatg cggctaatca gttcgacgcg atacggaacg
     6721 agatttccgc ttttgtgctg gttgcacacc acgcattgct tgtgaatatt gcgttcatca
     6781 aatcggagtt gaggtgccgc agcagttgtc cggtaatgtc cggcatccca ctgagcagac
     6841 gtgagcgttc cgcacgagat acatggtaag tcgcggtctc tttctctgat gaaggcgttt
     6901 acggcttgtt gggcttgttt aatccagtaa ctgcggggct ttaaggcgag ttttcgaatc
     6961 ttaagtttat ctttctgttt ctgctcctct cgtcgtcgtt tcttctctgc tgctttttcc
     7021 gctttttcgc gttctttact tcgtcgttcg agtgctatct tggttccaca ctctggagag
     7081 caccaccact gattagcgaa tgcagggtga aaccattccc gacattcatc gtttttacat
     7141 cgtcttcgcg ctggtttagc catcatcttc ttcctcgtgc atcgagctat tcgaatcgct
     7201 catcagctct gcacagcagt gctcacacac gtgaacttcc agcacatgca gcttctgacc
     7261 gcaattagcg cacgttaaag cccgctcgac gctttcttgt tcgtaacttc gatttg
//
LOCUS       DAHBHC010000149         7150 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHBHC010000149 DAHBHC010000000
VERSION     DAHBHC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.152"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            450..938
                     /locus_tag="M2A35_004782"
     CDS             450..938
                     /locus_tag="M2A35_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCB7200969.1"
                     /translation="MKKVHLLFILSTLLSVQSALATQQQPQYQDMPSDIAKSLVTTAL
                     KICHSKNKTGVVAVVDKGGNLVALQRDENVGPHNTLAAQKKAYTALSTKSDTRELAKK
                     AASDPETKNLNTVPQLLLLGGGVPVKSGNMVIGAIGVAGTGGPADDQHCAQEAIKEVM
                     GN"
     gene            complement(1166..1282)
                     /locus_tag="M2A35_004783"
     CDS             complement(1166..1282)
                     /locus_tag="M2A35_004783"
                     /inference="COORDINATES: protein motif:HMM:NF022969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200970.1"
                     /translation="MRPEYANSVESLIRVSHIIAINFQTIAAANRYWENNNI"
     gene            complement(1320..1424)
                     /locus_tag="M2A35_004784"
     CDS             complement(1320..1424)
                     /locus_tag="M2A35_004784"
                     /inference="COORDINATES: protein motif:HMM:NF022969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200971.1"
                     /translation="MNSKRRNMNDNWLPSLKNRTPLEGFGLAIMLSRR"
     gene            complement(1535..1756)
                     /locus_tag="M2A35_004785"
     CDS             complement(1535..1756)
                     /locus_tag="M2A35_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCB7200972.1"
                     /translation="MVFKVKIVKTILFFCGMALIPAVSFAQSISATSSTLEEAEAKIA
                     MKAQQEGLKYKIIEASNNNRIHMTAILYR"
     gene            complement(1996..6087)
                     /locus_tag="M2A35_004786"
     CDS             complement(1996..6087)
                     /locus_tag="M2A35_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7200973.1"
                     /translation="MNKIYALKYCHITQTIKVVSELARRVSRKSVSGKKKLCLPVSFT
                     IIMISPFAIASVVKGNIPYQTYRDFAENKGVFQAGKSDIIIKDKNGNITGTLNSAPMP
                     DFSATDTSGVATLVSPQYLSGVRHNGGYTGVRFGDGENLYHLVSRNNASDMDIHTPRL
                     DKLVTEVVPVGAASTDASFNHSSQYSAFYRLGSGSQLIKNDDGKNISITGAYQYLTGG
                     TVAGLSYYNWFGGTLMASTADLTSAQGVLPSHTQGGDSGSPLYAYDKAQGKWVLVANL
                     SSGSGKNALWSVVSADRIQNIMDAYSDGLVNYDNTSPENIIWSFDASEGVGSLSQGNE
                     AHTMHGKKGNDLNAGKDLSFSGHNGVIDIRDNVSQGAGSLTFHDDYTVTTTNGSTWTG
                     AGIIVDQNTSVNWQVNGVKGDNLHKIGQGTLIVQGTGVNEGGLKVGDGTVILNQQADS
                     SGQVQAFSSVNIASGRPTVVLADNRQVNPDNISWGYRGGILDVNGNDLSFHNISAADY
                     GAQLHNSSDKEATVSLTIPDAIEWNGKDTQRISGQVYKYFNTESQTTEFFVLKTTSAG
                     WSPSPAEKHLPEIFRGADYFSSLEEANREASADSQLIYHGKLTGNIRFDAGRVGKFVM
                     DGSADIGGTFSKEDGRLTMQGHPVIHAFNSQSIADKVAATGDGSVLTQPTSFTQDDWE
                     SRTFSLGMLELKNTDFGLGRNAALNTHIQADNSRVTLGDSRVFIDRNDGNGTAFKLEE
                     GTSVAVKDADKSTFNGTVSLTNHSVLNINEFFNGGIQADNSTVNISSSDAVLDSSTLT
                     GTALNLSEGADVLVRQNIISDGAVNIADAALSLNSRSGEFSRTLLPLYSYAGTWNLTG
                     DNASLYAGPFTLLSGDITVQGKGRVTIGGEGELAPDLPLQDRMFYSVFNGYRNVWGGR
                     LDAPDGNISMTDSQWSVKGNSTAGHLKLKRTMVGFGGDTSSFTTLTTGSLDAVQTSFA
                     MRTDLKNADRLVITDSATGHDNSILVNFQKKPSDKDSLSIPLVSAPKTAADDLFRAST
                     QVVGFSNVTPTISIREDDDKKEWVLDGYQIAPDEGQSRAAATFMHISYSNFITEVNNL
                     NKRMRDLRDINTEAGSWVRLMNGSGSGEAGFSDRYVLLQMGADRKHELDKLDLFTGIM
                     GTYTDTDVSAGMFSGKTKSWGAGIYASGLLPSGAYFDLIAKYIHNENRYELNFAGAGK
                     QNFRTHSLYAGAEAGYRYRLTETTFVEPQAELVWGKLQGQTFNWHDGGVDVSMRRDSV
                     SPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADIHLQDTAGEHHLNGGKDSR
                     MLYGVGLNARLGDNIRFGLEAERSAFGKYNVDNMINAGIRYSF"
     gene            6470..6994
                     /locus_tag="M2A35_004787"
     CDS             6470..6994
                     /locus_tag="M2A35_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HCB7200974.1"
                     /translation="MKKRILSLSIIFTCFSSFAQSINIPINIITDEGVGKNIGVVNIA
                     ESPYGLLFTPDLKELSPGMHGFHIHENASCAPGLMDGKKVAGLSAGGHFDPEKTGKHL
                     GPYNADGHLGDLPPVYVNNNGTATAPVLAPRLKSIKDISQHAIMIHKGGDNHSDSPLP
                     SGGGGGRIACGIIN"
BASE COUNT         1947 a         1745 c         1487 g         1971 t
ORIGIN      
        1 gaactgaaat acattcccgc gcataataag cgcacactct cagagaatat tgcgttgcat
       61 ttacaaatac gaagataaat aaaaaagcgt ccctgtacac ggagggtaat gggttcagaa
      121 atacgattga aaccgtgttg ccattgataa tctgagtgtc cgtatgtatt taaagccctc
      181 tgaaatacag gagcacgacg acatgataca ggtatccgct gtaagccaga acagatgctg
      241 tttgtggtgt cagactggac accagtacgg aaagacgtgc tcctctcgtt taccggagag
      301 actgcagatg aagacagcat acgcgctgaa cgtcagtcaa cacggagggc attacttcac
      361 gcaaatgata aagaaagtcc tgttgttcgc tggcgttgca tcgttacaga ttgttaaact
      421 ggtgtttcag aagattgtcg gagttaaaaa tgaaaaaagt gcacttgtta tttattcttt
      481 ccactctgtt gtcagtacaa tccgcgctgg ccactcaaca gcagcctcag taccaggata
      541 tgccctcaga tattgcaaag tctctggtca ccactgccct caaaatatgc catagcaaaa
      601 ataaaaccgg cgttgtggct gttgttgata aagggggtaa tcttgttgcc ttacaacgag
      661 atgaaaatgt aggtcctcat aatacactgg cagcacaaaa aaaagcgtac acagcccttt
      721 caactaaaag cgacactcgt gagcttgcta aaaaagcagc gtctgacccc gaaactaaaa
      781 atctgaatac tgttcctcag ctccttttgt tgggaggagg tgtacccgtt aagtccggta
      841 acatggtgat tggtgcaatt ggtgtggccg gaacaggcgg ccccgcagat gatcagcact
      901 gtgcgcagga ggctattaaa gaagtcatgg gtaattaaca ttcccccgtt aaaaggcctg
      961 cctcttatat aaaaatctgc cggcaacagt gtcggcagat ttttttgcat ccagacctgg
     1021 ttatacttct gtttttgcta tggcaggcag actggcgtgt cagtaaaaag tgaagtgacc
     1081 gcctggacgg tcacttctga gaaaaggcat ttacacagaa tcttaaacag gctcacagaa
     1141 ctatgtgcat gaacattatg agtggttata tattgttatt ttcccagtat ctgttggccg
     1201 ctgcaattgt ctgaaagttt attgcaataa tatgtgatac gcgaatcagt gattcgacac
     1261 tgtttgcata ttcaggtctg attttttaat acggctgtat caggccgggg cgtttttact
     1321 catcttcgac tcagcattat tgcaagcccg aatccttcca gtggagtccg gtttttcagc
     1381 gaaggaagcc agttatcatt catatttctc cttttactgt tcaatatctt gactcaagtg
     1441 atattcctct cttcatactg actgattaat cagtcagtat gagtaaagat aacatacaac
     1501 tgttgtcagg aaacctgatt aatcataatg aacattatct gtataatatg gctgtcatat
     1561 gtatacggtt attgttactg gcttcaatga ttttatattt cagtccttcc tgctgagctt
     1621 tcattgctat ttttgcttct gcttcttcaa gagtggacga cgtggcggaa atgctctgtg
     1681 caaaagagac agccggaatt agcgccatac cacagaagaa taagattgtt ttaactattt
     1741 tcactttaaa taccattcat cttataacaa ttactgtttg catagtaaat atattctaaa
     1801 aagagtcaat ctattaaact gataacttat atctgtcata ttcgttgatg ctgcatttta
     1861 tacagggtgg ataagttatc ctggaggcag ggaaaacagc acaacagggg aaactgcact
     1921 taaaaacact tattccacgt atttccttcc atggaaatac gcaacaggct caagaacgat
     1981 gtactgttat ttgtatcaga acgaatatct gataccggca tttatcatat tatccacatt
     2041 atatttgcca aatgcagaac gttccgcttc cagaccaaag cgtatattgt cgcctagccg
     2101 ggcatttaac cccacgccat acaacatacg actgtctttt ccaccgttaa gatgatgttc
     2161 acctgccgta tcctgcagat gaatgtcagc actgtccgtc aggtcgaact cataatgcag
     2221 cccggcacgt gccgtcagac tccagtcctt accactgaag gttttacctg aaacaacccc
     2281 cgttcttccg accagtggac tgacgctgtc acggcgcata gagacatcca ctccaccatc
     2341 atgccagtta aatgtttgcc cctgcagttt tccccagacc agttcagcct gaggttccac
     2401 aaacgttgtc tctgtcagac gatagcgata accagcctcc gcacctgcat acagcgaatg
     2461 tgtccggaag ttctgcttac cggccccggc aaagttcagc tcataccggt tttcattgtg
     2521 tatatatttg gcaatcagat cgaagtaggc gcctgacggt aacaggccac tggcatagat
     2581 tccagccccc catgacttcg ttttaccact gaacattccg gctgacacat cagtatcggt
     2641 ataggtaccc ataatccccg taaacagatc cagcttatca agctcatgct tacggtccgc
     2701 ccccatctgc agcagaacat agcggtcgct gaaaccagcc tccccagagc cggagccgtt
     2761 cattaagcgc acccagctac cggcttcggt attgatgtcc cgtaaatctc tcatacgttt
     2821 attcagatta ttgacttcag taatgaagtt gctatagctg atatgcatga atgtggctgc
     2881 agccctgctc tgtccttcat caggagcaat ctggtaacca tcaaggaccc attctttttt
     2941 atcgtcatct tctctgatgc tgatagtggg tgtcacatta ctgaatccca caacctgtgt
     3001 tgaagctctg aacagatcat ctgctgccgt tttcggggcg ctgaccagcg gaatactgag
     3061 cgagtccttg tcagacggtt ttttctgaaa gttaaccagt atgctgttgt catgacctgt
     3121 cgcggagtcc gtgataacga gacgatctgc atttttcaga tccgtcctca tggcaaacga
     3181 tgtctgaacc gcatccagac tgcctgtcgt cagggtggtg aatgatgatg tatcaccgcc
     3241 aaaaccgacc attgtccgct tcagtttaag atgccctgcc gtggagttac ctttcactga
     3301 ccactgagaa tctgtcatac tgatattgcc gtcaggcgca tcaagacgtc ccccccagac
     3361 attccggtat ccgttaaaca cactgtagaa catcctgtcc tgcagaggca ggtccggagc
     3421 cagttcaccc tcaccaccaa tggtgacgcg tccttttccc tgaacggtaa tatccccaga
     3481 taacagagtg aacggaccag cataaagact ggcattgtct cccgtcaggt tccatgtccc
     3541 tgcataactg tacagcggta aaagcgtacg ggaaaactca cctgagcgac tgttcagact
     3601 cagtgccgca tcagcaatat tcactgctcc gtctgaaatg atgttctgtc tgacaagcac
     3661 atcagcacct tcagacagat taagtgctgt ccccgtcagt gtggaagagt ccagtacagc
     3721 atcagaggag gatatattta ccgtactgtt atcagcctgt attccaccat taaaaaactc
     3781 attaatattc agcacagaat gattagtcag actgacagtt ccgttgaacg tgcttttatc
     3841 tgcatcttta acagccacag aggtaccttc ttccagctta aaggctgttc cgttgccgtc
     3901 attcctgtca ataaacactc tgctgtcgcc cagtgtgaca cgtgagttat ccgcctgaat
     3961 gtgtgtgttt agcgcggcat tgcgtcccag cccaaagtcg gtatttttta actcgagcat
     4021 ccccagactg aatgtcctgc tttcccagtc atcctgggta aatgacgtgg gttgcgtcag
     4081 tacggaacca tcaccggtag cagcaacctt gtctgcaatg ctctggctgt taaatgcatg
     4141 aataacggga tgcccctgca tggtcagacg cccgtcctct ttgctgaaag tccccccgat
     4201 gtcagcagag ccatccataa caaatttacc caccctgccg gcatcaaaac ggatattacc
     4261 ggttaattta ccatggtaaa ttaattgact gtcagccgag gcttcccggt ttgcttcttc
     4321 cagtgagctg aaataatccg cgccccggaa tatttccggc aggtgttttt ctgccggaga
     4381 gggactccac ccggcactcg ttgtctttaa tacaaagaat tctgtcgttt gtgactccgt
     4441 gttaaagtac ttatacacct gacctgaaat tctttgtgta tcctttccgt tccattcaat
     4501 ggcatccggt atcgtcaggg aaacagtcgc ctctttatca ctgctgttgt gcaactgcgc
     4561 gccgtaatct gcagcactga tattgtggaa ggaaagatca ttcccgttta catccaggat
     4621 cccgccacgg tatccccagc taatattatc cggattaacc tgccggttat ctgccagcac
     4681 gacagtcggg cgaccactgg cgatattcac actactgaaa gcctgaacct gccctgaact
     4741 gtccgcctgc tgattcagga tgacagtacc gtccccgact ttcagccctc cctcattcac
     4801 accggttccc tgaacaatca gcgttccctg gccaatttta tgcagattgt cacctttcac
     4861 accattaacc tgccagttca cggacgtatt ctgatccaca ataatcccgg ccccggtcca
     4921 ggtactgcca ttagtggttg tcaccgtata atcatcatgg aatgtcaggg aacctgcgcc
     4981 ctgcgacaca ttatcccgga tatcgataac accattatgt ccgctgaaag aaaggtcttt
     5041 accggcgttc aggtcgttcc cttttttacc atgcattgta tgggcttcat ttccctgaga
     5101 cagactgccc accccttcgc tggcatcaaa cgaccagata atattctcag gagaagtgtt
     5161 gtcataattc accagaccat cagaatatgc atccatgata ttctggatcc tgtcggctga
     5221 aaccacagac cataaggcgt tctttcctga accggaagac agattagcga ccaataccca
     5281 tttaccctga gctttatcat aagcatacaa aggtgagcca ctgtctcctc cctgagtatg
     5341 agaaggaagc acgccctgag cagaggtcag atctgcagtg gatgccatca gtgtaccgcc
     5401 aaaccagttg taatagctga ggccggcgac cgttcctcct gtcagatatt gataagcacc
     5461 ggtaatactg atgttttttc cgtcatcatt ttttatcaac tgagagccgg aaccgagtcg
     5521 atagaaagcg gaatactggc tgctgtggtt aaatgacgca tcagtgctgg cagcccccac
     5581 cggcacaact tccgtaacaa gtttatccag tcgtggagta tgaatatcca tatctgaagc
     5641 attgtttctg ctgaccagat gataaagatt ctctccatca ccaaatctga cgcctgtata
     5701 tccgccatta tgccggacac cggacagata ctgagggctg acaagtgtgg caacaccact
     5761 cgtgtctgtt gcactgaagt ctggcatcgg agcagaattc agcgttccgg taatatttcc
     5821 gttcttatct tttattatga tatccgactt tcctgcctga aatacccctt tattttccgc
     5881 aaaatcacgg tatgtctgat aagggatatt ccccttgaca acggaggcaa tggcaaacgg
     5941 cgatatcatt atgattgtaa aagacaccgg aagacacagt ttctttttac cggagactga
     6001 ttttctgctc actcttctgg caagttctga aacgacttta attgtctgag tgatatggca
     6061 atacttgaga gcatatattt tgttcattaa ataataatcc tgattttatt cctgatatta
     6121 ctgacgaact gttacacata aaaaatatac aaagcggtgt ttttatggca ttacagagac
     6181 atgctggtaa taacgtgtgt ctgcacatct gtatgattgc cgcacaaaac aagtcgttct
     6241 gatttctgtt ttatataaca acagagttaa tatgtataaa agtaagtcag aaacactctt
     6301 tattatgcga tataagtctg gataaatcca gataaatcaa ctccatactt tttgccatat
     6361 gtcaggataa aacacaaaga ctatcacact aatagacaaa aacaatttta tgaagatagc
     6421 ttgtaccata aaataacaac caatataaaa cagtttgtct ggatatacta tgaaaaaacg
     6481 aatcctttca ctgtcaataa tatttacctg tttttcctca ttcgcgcaga gtattaatat
     6541 cccaataaac atcattacag atgaaggtgt cggtaaaaac atcggcgttg tcaatatagc
     6601 tgaaagccct tatggcttac tgtttacacc tgatctgaaa gaactgtcac ccggtatgca
     6661 cggttttcac atccatgaaa atgcgagttg cgcaccgggt cttatggatg gaaaaaaagt
     6721 agccggactt tctgccggtg gtcacttcga tccagaaaaa accgggaaac accttggtcc
     6781 ttataacgct gatggtcatc tgggtgatct ccctccggtt tacgtaaata ataatggaac
     6841 agcaacagca cctgtattag caccaagatt aaaatcaata aaagatatca gtcagcatgc
     6901 aattatgata cacaaaggcg gtgacaatca ttcagactca ccactcccgt caggaggagg
     6961 gggaggccga atagcctgtg gcatcataaa ctaatatatc atatataccc tcagactatt
     7021 tacagcgtcc tctgaaacca ttaccctccg tgtacaggga cgctttttta tttatcttcg
     7081 tatttgtaaa tgcaacgcaa tattctctga gagtgtgcgc ttatgatgct cgggaatgta
     7141 tttcagtgcg
//
LOCUS       DAHBHC010000150         7102 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHBHC010000150 DAHBHC010000000
VERSION     DAHBHC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.153"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..3357)
                     /locus_tag="M2A35_004788"
     CDS             complement(<1..3357)
                     /locus_tag="M2A35_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCB7200975.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNCDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADISQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLDQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHM"
     gene            3491..4018
                     /locus_tag="M2A35_004789"
     CDS             3491..4018
                     /locus_tag="M2A35_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HCB7200976.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            complement(4209..4841)
                     /locus_tag="M2A35_004790"
     CDS             complement(4209..4841)
                     /locus_tag="M2A35_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCB7200977.1"
                     /translation="MCLHGDLQRFGRRISLYVNTAAEAIRALSLQVPGFRGQMNEGWY
                     QIRIAGEDTAPEAVYARLHEPLSGRAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(4787..5530)
                     /locus_tag="M2A35_004791"
     CDS             complement(4787..5530)
                     /locus_tag="M2A35_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7200978.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNLAEN
                     GFCRVSPSSAQAGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            complement(5536..6234)
                     /locus_tag="M2A35_004792"
     CDS             complement(5536..6234)
                     /locus_tag="M2A35_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCB7200979.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(6234..6563)
                     /locus_tag="M2A35_004793"
     CDS             complement(6234..6563)
                     /locus_tag="M2A35_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7200980.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(6560..>7102)
                     /locus_tag="M2A35_004794"
     CDS             complement(6560..>7102)
                     /locus_tag="M2A35_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7200981.1"
                     /translation="EQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGAS
                     ASTGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLM
                     RGYAEGGYVGGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKG
                     AQDEIQAQMRDGGVFSGGRR"
BASE COUNT         1468 a         2269 c         1787 g         1578 t
ORIGIN      
        1 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
       61 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagta
      121 aatatcagta tgctctctct catgtttagc gccgctgaat gccaccgccg gaataacaat
      181 ttgccggtca aacggctgat cgtcataaac cctgacggta atggtccctg atggccaccg
      241 ctccggtgca cgggaatccc gggggaaagc tttgcccact gttttaacga gatcgccttc
      301 aatctggttc gcggacagtt ttcccagaac ccgacagttc tcgttaatcg tgacgttgtt
      361 gagcgtcccg gaattcgcat tcacgttacc gctgatatca gcatttcttg cggtcagcct
      421 gccctccggc gtcagggaaa acgtcggggg attgccggac gaggtgatac tcaccgcaaa
      481 cagccgcttc aggaacacat cgttcatgaa caactgattc ccctgcgcca caaataacgg
      541 cgtggtgttg ccgtcctccg ggttaatcat cgcaatacgg tcagccagca gcagtatgtt
      601 gctcaggggc tggccatcag tatcctcaat ccccgctcca ataccggcaa catagggtat
      661 gccatttttt gttttctgta ccttcagcat gtaaagtgca gcaaggtcat catttgtgtc
      721 cttctgcacg cgctgtatct gctgtatggt ggcgctctgg tcttccagcg ttttactgac
      781 cgtctgtgtg atttcattgc gggtttcggt gatggtggtc ttcatctccg ccatctcatc
      841 cgcaagctgg ctgttgtcta tcagctccca cagcccctga tccagatgca gttttcctat
      901 tttttcccga aacagtccca gatacccttc tgcatcattg ctggcccggc cactggcttc
      961 cacaaaagca gatttcccca ccaggttgac gctgcgcacg taaaaccaga aatccttccc
     1021 gggcttaatg tgcgggccgg atacactcca ctgactgccg gtccccagat aacgggcaga
     1081 ggtttccacc tgagatatgt ctgcgatttt tgcctccgaa aaccagaact caaactgtac
     1141 cgtcgggtca tacaccgcaa gacgcgggac cgctgttatc tgaaaatagc ccggtgtcag
     1201 ctcaatcgtg gcgggtaccg caggtgcatt aatcctgaac gtggtggtgg ccggttcgcc
     1261 ctgctggcca taactgttaa ttgccctgac tgtcagggtg tattccccga gcggcagacc
     1321 actgaaacga tgctctgtat ccgcagtgat ggcggtggtc accagacggc tgtcttctcc
     1381 gcttccgctg gtcaggcgca gactgaagcg cacacccttc accacccgcg gcgtgtccca
     1441 tttcgcctgt gccagatact gaccgtcagc cgcgctcacc tccaccgtca ggtgctgcac
     1501 tgccggtgga ataacgctgt tcagggtgcc tgactgcggc tcaaagctgg ccccgttatc
     1561 cacgatggct tctttttccg gtacgtgctg caccgccgtg atggcaaagg tgccgtccgt
     1621 gttttcccgg atggaaacac agcggaacag gcgacgacgc agtgacggca gggagagtcc
     1681 ccatacaccg tatgtctcca caccatcagg cagggtgctg acctgtatcc ggtccggcgc
     1741 ggggtgtgca gtgatggcca cactcaccgg cttaccgctg ccgttaatca ggttcaccgt
     1801 ggcggcacct gtctccggca gggtcacctc acggtccagt gtcagggtgc ggctggcggc
     1861 atcgatggac aggatacgtc cgccggtcat ggtcccggca tagtcgttat cacagatttc
     1921 aataatgtca ccgggtgtgt gacgcagccc ctgtgacccg agcgtgaaat ccaccgtctg
     1981 cgtttccagc agtccggtct ttatcaccca cagcccggca cggtgggcct gaccgcgact
     2041 ggtgcaaccg aacgcatcca tcttcagcag gttgcgcccg tagcgcagta tggcttccgg
     2101 gtcttccacc agttccgtgg aggtctgcca gccgttctgc gggtcggtgt aattcacctc
     2161 caccgccgtg tggcggtcct tcagggcgct gaagctgtag cgaaacccca cgccgttatc
     2221 atccaccacc acatcgcagt tggtgtacgg ccacaccaca tccgacgggc ggtcctgaac
     2281 gaacgtcagc gtctggccgt tccataccgg catacagcgc atcgccgagc agaaatcact
     2341 gagaacgtcc cacgccttac gctgttgtga caggtacgca ttaaaggtca tccgcggctc
     2401 tgtgcccccg aaaccatccg ggaccgtctg gtcgcagtac tgcgcaatgg catacagcgc
     2461 ccatttgtcc acgtctgccg cccccagacg ttttcccatg ccgtagcgcg ggtgagtcag
     2521 catgtcccac aggcaccagg ccgggttgtt gctgtatgcc ggtttcaggc tgccgtccca
     2581 gataccgctg tacgtgcgtt tttccgggtc atagtttgac ggtacctgaa tgatgcgacc
     2641 acggatatgg tagttcaccg tcatctgctg gccgccgaac tgctccgcat ccacctgcag
     2701 ccccacaatc gccgtgttcg ggtagcactg tttcacatcg atgatttcgg tgtatgacga
     2761 ccagagcgtc ttattctgca gctggtccga ggtgctgtcc gctgtctccc ggaccatccg
     2821 gatgttaaag ggccgctcag gcagattatc cagaatcacc gacgccagaa actgcgaggt
     2881 ggtcttgccg ttaatggtga catccttttc cgtcacccag ttaccgttac gctgtaactg
     2941 aatcagcagg cggacggatg tcgggtttcg gtcacccttt gacgtggtct gcaccagtga
     3001 ctgcaccccg aaggtgaccc gcaggcggtc aatgttcgcg gatgtaatgg tgcgcgtcac
     3061 cggctttgcc ttcgtcactt ccacgcccag tgcggtttcc gccccggagg actcaaagcc
     3121 ttcaggtggt gtctgctcct gctccccggc gcgccagacc gcggtcacac catgtatcac
     3181 aggattaccg tccgtgtccg tcagcggggt tttgttcacc aggatactct gcagcccctt
     3241 caccggacct tcaatcggcc cttcaccaat ggcgtcaatc acgctcatca tctgcgtgga
     3301 tttgagattg tccttcgcct cacgaggtgt gtgcgccctg ccgccacctt tacccataat
     3361 gttcctctca attggtatta ttaatcgcag tgataggata ttgcacagct attgcgcgat
     3421 atcatcagaa cgctgtttgt taccctgtaa ccagcaagct cagtctgtta acggaattaa
     3481 tgagggtttt atgaaatgta aaatcattgc tgccattgcc atgctgacag cagcatcatg
     3541 cggatacgca gcagaacagg aagtcccaat gaaccttgtc agtgctgacg gaaaagaagt
     3601 cagcattgga aaaataacca ttcaggagac cccctacggt ctgctgttca caccagccct
     3661 tcactctctg tctgaaggca ttcatggttt tcatgtgcac gaaaaaggaa attgcgcccc
     3721 ggcactgaaa gacggaaaac cggtcgcagc attatcggct ggcggtcact ttgacccgaa
     3781 aaacaccggc aaacatcttg gcccctggtc tccggatgga cacctgggcg acctccctgc
     3841 gctgttcgtg acgcatgacg gaaaagcgaa ctacccggtc ctggccccga gactgaactc
     3901 attaaaagag attaaagggc gttctctcat gcttcatgct ggcggtgata accatcatga
     3961 ccatccggag cccctgggcg gtggtggtgc gagaatggcc tgcggcatca ttcaataatc
     4021 agtcaggtaa ggggcgggcc ccttaccttt attccttagg acgataaatc ctttctccct
     4081 gaaaagaacg gcacatcctc cctctctgag ttaatgtttt tgtcgtgaca taagaataat
     4141 tccttacact caatcttcgt aactctcccg cagttcctgt ccgtgagcac tgcgggattt
     4201 tttcgctttt atgcctgccg cccgataacc accaccttcc cgtcaccgcc ttcatcacgg
     4261 gtactgatgt cctgggatat acggcgggag ccaaccagca tttcaccgta aggcaccggc
     4321 atcgggttcc cctgggcaat catgttatcc agcgaggaaa agtacgtgtt ctgtctgccg
     4381 ttatccgtgc ttttgtactc cggtacttta gccttcgggg ccagcatctg agccacacca
     4441 cccagaatca tgctggcccc cagagaaaac agcatcgtgg tggcagaaaa accaccggct
     4501 gccagggctg aaccccataa cgccattgat gccccggccg tgaagaacga ccccacgatg
     4561 gctgccgccc ccagcacaat ctgcagtccg ccctttccgg cccctgccag ccgcggtacg
     4621 atatgaatca cggccctccc gctcagtggc tcatgaagac gggcatacac cgcctccggc
     4681 gcggtatcct caccggcaat acgtatctgg taccagcctt cgttcatctg accgcggaat
     4741 cccggcacct gcagcgacag ggcacggatg gcctccgctg ccgtgttcac atacaggctg
     4801 atgcggcgac caaatcgttg taaatccccg tgaaggcaga tgcggaccag tggcggtgac
     4861 gccagacaga atgcgttcgt cgttgccatt tttcagaata cctctcccgt ttactcagtt
     4921 gttcaggtat atggtgaagc agttcaccgt tgccacagta aatggcggca tgattggcca
     4981 ccgatgcgcc aaagcagcac agcaggatat cgcctgcctg tgcagaggac ggagacaccc
     5041 ggcaaaagcc gttttccgcc aggttgtcca ggtacaggtt ctggccgttg cgccaccagt
     5101 catcctcacg ctcaaaatcc ggcatatcaa ttcccgccag atggtaggca tcccggaaca
     5161 gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgtcc tgtcagatgt ggcacacagc
     5221 ggaatttatg aatttccccc cggcagacca gccaccagga cagtgcactt tttatctgca
     5281 gccgccggtc ggcctcgctc agccagggca gaccaccggg atgactgtgg accagtgcca
     5341 caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcaatacga aaatacgcct
     5401 ccggctctgc agagatattc acacaaggga tataccgctc cccctccggc gttctcacca
     5461 cgaagccgca cgactccgca ggcacacacc gccgggcatg cgccagaatc gctgattctg
     5521 tctgtgtcat tggatttact gcgaaagttt gttaatggaa aggaaaccgc caaaattagc
     5581 caccatgccg cgcatctcac acccgcgcat gcacttgctg catctgtcct tacggatatc
     5641 ggtggtgggt ttatcgaact catccgccac cgcaggaccg ttatacccgc attcatctcc
     5701 ccggtaatcc cacatacagg tgttcgccag catgatgcga ccgggaaaca gcgctccgtc
     5761 cgtctccgtc ggtgttgcca gcacaaacga ggctgtcatg gccgtcagct ctgacatctg
     5821 ctccaccacc cagcggtcgc tcagctcctg ctccgggtcc gcttccggat tgcccgccac
     5881 aaaattcacc gcatccagaa aacgggcata cacccggcgg cggaccaccg tggcccccac
     5941 caggctctgc aggtcctccg ccattccggt gaccagaccg aacagattcg acaccgtcag
     6001 cgacgggcgg gcactgctgc ccttcccgtt catctcaaag ccactgccgt caatcgggta
     6061 tgcctgatat tgccgcccct gccaggtaac cgcctcccct ttttcattca gctcattgca
     6121 gaaaaaatac cgctcaccac cctgtaccgt caggtcgatt tcccagagta ccacccgcgg
     6181 tgactgctct gatttaaccg actcgttcag actttcttcg tgaatatcct gcatcagttc
     6241 accacctgct taaactccgc gctgaattca acccgcagca tgcgaacccg tgatgaccag
     6301 gcggcacagg tcacctttat ctgccgccag gcataaggcg gtgtccacag aaacgccttc
     6361 cagccaccgt gctctgccag gaatgcctcc agatgtcggg cctcctcccg ggtcacggaa
     6421 agcgtcacac ggtatgtttt caggtcagca ttcagccccg ccgccatacg ctgcgaatac
     6481 ccgtcaccaa aacgcacttc acgcaccgat ggctgcgagt tcacctccat atccggcttc
     6541 actttccagc gaaatgtttt catcgcctgc ctccggaaaa gacgccgcca tcacgcatct
     6601 gcgcctgaat ctcatcctgc gcccccttgc gggccatgtc atacaccgcc ttcatcagct
     6661 gcggccccgc ctgtccgttg gtgccgtcgt tctgaatcac cacgtgattg ttctgattaa
     6721 aactaatgcc ttccgcccgc cgcatctgcg ccggacttcc ggcaccgccc acataaccac
     6781 cttccgcata cccgcgcatc agacgataca ggttgccgac gccaatccgg ctggttgcct
     6841 ccttcgtgaa gacaaactcc ccgcggtgga caataccggc aggttcgtat ttgccaccgg
     6901 ttcccgtaaa tcctccggtc gcgaaatgga agttcgccgc cgcagcctga atggccgtcc
     6961 ccgtggaggc agacgcccca ccaccgaaag caccacccat ggcgctgcca atactcccga
     7021 caatccccac catggcctgc ttcagaaaaa tctctgtcag catggagagc acagaacggg
     7081 tgaaaccacg ccagctctgt tc
//
LOCUS       DAHBHC010000151         7019 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHBHC010000151 DAHBHC010000000
VERSION     DAHBHC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.154"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            22..867
                     /locus_tag="M2A35_004795"
     CDS             22..867
                     /locus_tag="M2A35_004795"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCB7200982.1"
                     /translation="MLVRRLTPRECERLQGFPDDHTKIPWRNKLTEDCPDGARYKAIG
                     NSMAVPVMRWIGEQIKRATATQEIICNPYVAAVDKLRAQNEHYLREVGDQWRTPDLLF
                     WGINAIFGPLVLDLFTDGDNSKCPNYYTAEQNALTQDWSARLADLGGAAFANPPYSRS
                     QYHDGQAITGMAHIMSHARSMRDAGGRFVFLLKAAPSETWWPEDADHVTFIRGRVGFD
                     LPAWFNPADEKQAPSSANFAAAIVVLDKNWRGGRFGYIHRTELEAKGRAALALARYAV
                     NEAIF"
     gene            864..1604
                     /locus_tag="M2A35_004796"
     CDS             864..1604
                     /locus_tag="M2A35_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7200983.1"
                     /translation="MKLESLPKFYAPKSPNLGATAPATGGDRMTITDVMTAQGFVQSK
                     TPLGFALFLAKAGIQTPEPAIELLAKIAWELAPACRAVNVLPGNQRAAVCDVLATMAY
                     QDYSRSAASVRPCDCCAGDGFITAEVFTMKMAGVVHRQVREIERILCANCKGKRVISN
                     SCRCHGKGRVLDASGEVLLWKECDRCRGRGYSRLKFSAVLVALQRVWPELKKTTAYNE
                     VQPFFEILVTHCHREEAAAEQQLQVVTR"
     gene            1872..2636
                     /locus_tag="M2A35_004797"
     CDS             1872..2636
                     /locus_tag="M2A35_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200984.1"
                     /translation="MQGQPKTKHQELVETLSLYINSRTPLEEPLLGELLREINKQSVA
                     SRAYLMALWMEAQNRHDETVAWYLEAIATSGEHASVVAGNYLSYLSCSAHNLFHRTEL
                     FRLVDIYCTKRIRRMARNAAFCMGNDRLVNRFTVMMKALLDGQEREEIEREGAKMVEA
                     IVTFKEATKLTSSEIQKLCDSAEQIANSHGVNCAGVEYFLSGGYDNAIILFADTSDSS
                     TLTTMNMELIGLLADDPYIDRPFTSWFKSSDMKDRV"
     gene            2636..3037
                     /locus_tag="M2A35_004798"
     CDS             2636..3037
                     /locus_tag="M2A35_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200985.1"
                     /translation="MSVSGQDFINFAEKCLEFNDEIGFRNVVGRSYYGVYHEICSKLE
                     HCYVLTSHEGVRNYLMSSTQCKKEPFDKAGLRRIGAFLHSLHVQRKWADYTLERELVK
                     ADAESALNTAKQVMAEIKALHEAVYPPQPAA"
     gene            3272..3613
                     /locus_tag="M2A35_004799"
     CDS             3272..3613
                     /locus_tag="M2A35_004799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200986.1"
                     /translation="MLELYRQHKTVLDILLLVILGVIVRVFYWGGRFRECCGDILIGT
                     TIMVFAAARLPNITIPVPGDAPLIFSHNEIAFVVGIMGYKGLKEIIFWVLKTRFNIDL
                     RERIAQRKNSK"
     gene            3630..4115
                     /locus_tag="M2A35_004800"
     CDS             3630..4115
                     /locus_tag="M2A35_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HCB7200987.1"
                     /translation="MSEPAWVIEARKYIGERELKGPKHNSLILQMWRDIKRGGIKDDE
                     TPWCAAFVGAVLERCGIKSTRFESAGSYLGWGEQLLKPAFGCVVVFKRDSGGHVGFVV
                     GQNSAGDLLVLGGNQSDAVNIKAFPRSRVSGYRCPAGVQDIPDFNLPEQVNISFSISE
                     A"
     gene            4118..4306
                     /locus_tag="M2A35_004801"
     CDS             4118..4306
                     /locus_tag="M2A35_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7200988.1"
                     /translation="MKRLLILAVMLLAGCTHTTYTEAKRADGTTVKHIAIAPGTKITT
                     ASGGCIDSTGAAAECPIK"
     gene            4624..5130
                     /locus_tag="M2A35_004802"
     CDS             4624..5130
                     /locus_tag="M2A35_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCB7200989.1"
                     /translation="MDQELKNSRLNINQIASLTDLHRQTVAARLSGVPLAPGSNPKLK
                     LYSVVDILTTLLTRTTDPAKINVDELLPTDRKAWFQSERERLKFEIETATLIPASDVT
                     REFATMAKAVVQVLETLPDILERDCGLQPAAVARVQTIIDDLRDQIAQRVEQAETETE
                     QEDAPQED"
     gene            5130..7019
                     /locus_tag="M2A35_004803"
                     /pseudo
     CDS             5130..7019
                     /locus_tag="M2A35_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145097.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
BASE COUNT         1766 a         1612 c         1936 g         1705 t
ORIGIN      
        1 ccgccagcgg tcgccaaggc aatgctcgta cgccgtctca ctccgcgtga gtgtgagcgc
       61 ctccagggat tcccggacga ccacacaaaa ataccctggc gcaacaaact gactgaggac
      121 tgtccggacg gggcgcgata caaagcgatc ggcaatagca tggcagtgcc ggtgatgcgc
      181 tggatcggcg agcaaattaa acgagcaaca gcaacgcagg aaattatctg taatccctac
      241 gtcgcggcgg ttgataaatt acgcgcgcag aatgaacact atttgcgcga agttggcgat
      301 cagtggcgca ctcctgatct gctgttttgg ggtattaatg caatatttgg cccactggtc
      361 ctcgatctgt ttactgacgg cgataattca aaatgtccga attactacac ggccgaacaa
      421 aacgcgctaa cgcaagactg gtccgcgcga ctcgcggatc tgggcggcgc tgcgttcgct
      481 aatccgccgt acagtcgatc gcagtatcac gacggacagg cgatcaccgg catggcccac
      541 atcatgagcc acgcccgctc aatgcgtgac gctggtgggc gttttgtttt cctgctgaaa
      601 gccgcgccat cggaaacctg gtggccggag gacgcggatc acgtaacgtt tattcgcggt
      661 cgtgtcggtt ttgatttgcc agcctggttt aatcctgctg atgaaaaaca ggcaccctcc
      721 agcgcaaatt ttgcggcggc aattgttgtg ctggataaaa actggcgcgg gggccggttc
      781 ggatatatcc accgcacaga gctggaagcg aaaggccgcg ccgccctggc gctggcacgg
      841 tatgcagtca atgaggccat attttgaaat tagaatcact cccaaaattt tacgcaccga
      901 aatcgccaaa tctcggcgca acagcgcccg ccaccggcgg cgaccgcatg acgattactg
      961 acgtaatgac ggctcagggg ttcgttcagt caaaaacgcc gctggggttc gcgttatttc
     1021 tcgcgaaagc aggcattcag acgcctgagc cagcgattga actgctggca aaaatcgcgt
     1081 gggaactggc accggcgtgc cgcgctgtta acgtgctgcc aggcaaccag cgcgctgccg
     1141 tgtgtgacgt gctggcaacg atggcctacc aggactactc ccgcagcgcc gccagcgtgc
     1201 gcccgtgtga ctgctgcgcg ggtgacgggt ttataactgc tgaggtgttt acaatgaaaa
     1261 tggcaggcgt ggtacatcgg caggtacgag agattgagcg catattgtgc gctaattgta
     1321 aggggaagcg agtaattagc aattcgtgcc gttgccacgg taaggggagg gtgttggatg
     1381 cctctggcga ggtccttttg tggaaagagt gcgaccgatg tagggggcga ggatactctc
     1441 gtttgaaatt ttccgccgta ctggttgcgt tgcagcgggt gtggccggag ctaaaaaaaa
     1501 ccactgctta caatgaagtg cagccatttt ttgaaatact ggtgacgcat tgccacagag
     1561 aagaggctgc agccgaacag caattacagg tggttacgcg ctgattattg ctaaaaaaag
     1621 gtgctaaaaa ttacattgtt gctattcatg gaaaaatagg ataaatttcc tccaacgata
     1681 ggcgttgtat gttcgttcct ctaagccccg ccacatgggg cttagtcaaa aaacacctct
     1741 tttttggttc tttttatcgt attttcccgt gacaagatca tgtttactgt taaaatcatt
     1801 caacatgatt cagtgatgtg aatcttttta aaaaaataaa aagcataaat ttcaaagagt
     1861 aagctcaaaa aatgcagggt caacccaaga ctaaacacca agagttggtc gagacgctat
     1921 cattatatat aaactcgcga accccgttag aggagcctct tttgggtgag ctcttacgcg
     1981 agattaataa gcagtccgtt gcctctcgtg catatctgat ggctttgtgg atggaggctc
     2041 agaacaggca tgatgagaca gtggcatggt atctagaggc gatagctact tctggtgagc
     2101 acgcttctgt tgttgcaggt aactatctga gttacctgag ttgctcagcg cataatcttt
     2161 ttcacagaac agaattattt cgattggtag atatatattg tactaagcgg ataagaagaa
     2221 tggccaggaa tgcagcattt tgcatgggaa atgaccggct tgttaaccga ttcactgtga
     2281 tgatgaaggc tttactcgat ggccaagaac gtgaagaaat cgagcgtgag ggggcaaaaa
     2341 tggtggaagc tattgtcact tttaaagaag ccactaagct aacttcatct gaaatccaga
     2401 agctgtgtga ttcagctgag caaattgcaa atagccatgg cgtgaattgt gcgggtgttg
     2461 agtattttct tagcggtgga tacgacaacg ccataattct gtttgcagat actagcgact
     2521 ctagcactct taccaccatg aacatggaac tcataggctt gcttgctgat gatccctata
     2581 tagaccgtcc atttacttca tggtttaaga gttctgatat gaaggatcgg gtgtaatgag
     2641 tgtatcaggt caagatttca ttaattttgc agagaagtgt ctcgaattta atgatgagat
     2701 aggtttcagg aatgtggtag gacgctccta ttatggtgtt taccatgaaa tctgtagcaa
     2761 acttgagcat tgttatgtgc tgacttctca tgaaggtgtt cgcaattacc ttatgagttc
     2821 tacgcagtgt aaaaaagagc cgtttgataa agccggactc agaagaattg gtgcatttct
     2881 tcatagtctt catgtgcaga gaaagtgggc tgattacact cttgaacgcg aattagttaa
     2941 agctgatgcg gaatccgcac tcaataccgc aaaacaagtc atggctgaaa tcaaggcctt
     3001 acacgaggcg gtttaccctc ctcaacctgc tgcgtaactc acgaaatccg acatggaaac
     3061 ccgcctccga gcgggttttt ttgtgctttt ttccgtaaat ctcattgcta acaccacggt
     3121 tggtgttaat tcctcgtagt gagatttgca gaaaaacagg tgttaccgcg tttccccgta
     3181 ttccccgggg ttttttattc aatctgccac cgctggtggt atccatttat tcgagtcgca
     3241 tatgccgcac acgtcgagct ggtggggatt aatgctggaa ttgtatcggc aacataaaac
     3301 agtcctcgat attttattgc tcgttatttt aggtgttatt gtcagggtat tttattgggg
     3361 tgggcgtttt cgtgagtgct gcggtgatat attgatcggc accactatta tggtttttgc
     3421 cgctgccaga ttgccgaata ttacgatccc cgtgccaggt gatgccccgc tgatattcag
     3481 ccacaatgaa atagcgtttg ttgttgggat aatgggttat aaagggctga aggaaattat
     3541 tttttgggtg ttaaaaacca gattcaacat tgatttgcgc gagcgtattg cgcaacgtaa
     3601 aaacagtaaa taatttttgg tgaaaaagca tgagtgagcc agcctgggtt attgaggccc
     3661 gaaaatatat tggtgaacgg gaattaaaag gcccgaaaca taattcgtta attttgcaaa
     3721 tgtggcgcga tattaaacgc ggcggaatta aagacgatga aacgccgtgg tgtgcggcat
     3781 tcgtcggtgc cgtgttggag cgttgcggaa ttaaatcaac tcggtttgag tcggctggct
     3841 catacctcgg ctggggtgaa cagctattaa aaccggcgtt cgggtgcgtt gttgtattta
     3901 aacgcgacag cggcgggcat gtcgggtttg ttgtcgggca aaatagcgcg ggtgatttgc
     3961 tagtgctggg cggcaatcag tctgatgcgg tgaatatcaa agcgttcccg cgctcgcggg
     4021 tgtctggcta tcgctgtccc gcaggcgtcc aggatattcc tgattttaat ttgcccgagc
     4081 aggtgaatat tagtttttca attagtgagg cgtgacgatg aaaagattat taattctggc
     4141 ggtaatgctg ctggctggtt gtacgcacac aacgtataca gaggctaaac gcgcggacgg
     4201 aacgacggtg aaacatattg ctatcgcgcc gggaacgaaa attaccacag cgagcggcgg
     4261 gtgtattgat tcgacgggcg cggcggctga gtgtccaatt aaatagacac tacagagcgt
     4321 cacgcgctgg cgctcgatag tgtttattat cagtgacgga ccgctggttt tatttaacga
     4381 ggtgcagtgg tgcaaaaaca aattattggt gacgcgggcg tgtaaatagg cgtgattaat
     4441 taattttgca tatggattat tgcaaggtac tccctagcct cagaaacacc gggaggcagc
     4501 agacgcgcaa aaaacgccgc gttttttgca ttttatcggc atcatcatca tgcggttaac
     4561 atattgaaat atttgctaaa aaaatatttc agatgtcgaa atggttaaat attgttcacc
     4621 atcatggatc aggaactcaa aaattcacgg ctcaatatta atcagattgc cagcctgact
     4681 gatttgcacc ggcaaacagt tgccgcgcgg ttaagcgggg tgccgctggc acccggcagt
     4741 aatccgaaat taaaactata ttccgtcgtt gatattctga cgacgctatt aacccgcact
     4801 accgaccccg caaaaattaa cgttgatgaa ctgctaccca cggatcgcaa agcctggttt
     4861 cagtccgagc gcgaacggct taaattcgag atcgaaacag cgacattaat acccgccagc
     4921 gacgttacgc gagagtttgc gacgatggca aaagcggtgg tccaggttct cgaaacactg
     4981 cctgatattt tggaacgtga ttgcgggttg cagccagcgg cggtggcgcg agtacaaaca
     5041 attattgacg acctgcgcga tcagattgcg cagcgcgttg agcaggcgga aacagaaaca
     5101 gagcaggagg acgcgccgca ggaggattaa tgcagcaaca ggcaacggcg cgaggggcgc
     5161 gaaaaaatat agcagcaatg atccgcgcgc cgcgcaggat gccggtttct gaggcggtcg
     5221 caaaatatat gcgcgtacca atgggcgcgg gcaactccgt gccgtgggat ccgctggtgg
     5281 caccctacgt tattgagcca atgaattgcc tcacgtcgcg tgaatacgac gccgttgttt
     5341 ttgtcggtcc ggctcgaacg ggtaaaacaa ttggcctgat tgacggctgg attgtctaca
     5401 acgtggtgtg tgatccgtcg gatatgctga ttattcagat gactgaggaa aaagcgcgcg
     5461 agcactctaa aaaacgccta gcgcgtacat tccgtgccag cccggaagtt gcgtcgcggc
     5521 tgagtccaaa ccgcaacgat aacaacgttt atgaccgtac attcctggcg ggcaattatt
     5581 taaaaattgg ctggccgtca atcaatatca tgtcgtcgtc ggattttaaa tgcgccgcgc
     5641 tgactgatta cgaccgaatg ccggagaata tcgacggtga gggtgacggg tttacgctga
     5701 cgtcaaaacg aacgaccacc tttatgtcca gcggcatgac gttggttgag agttcgccgg
     5761 gccgcgatat taccgacgtg aaataccgca aaaattcacc gcacgaggca ccgccctgca
     5821 cgggtatttt atccctgttt aaccgtggtg atcggcgttg ttggtactgg cagtgtccgc
     5881 actgccgcga atattttcgc cccgttggcg acaccgttac cgggtatcag gatatcgcag
     5941 atccggtgct cgccagcgag gctgcatacg ttgaatgccc gcactgccag ggcaaaataa
     6001 cccccgatca gaaacgcgaa ctatggctgc gcgacggaga aactatcgat gcagacggca
     6061 atcggggtgg agagccgcgc cgctcgcgca tcgcgtcatt ctggatggaa gggccggcgg
     6121 cggcatatca gacgctgaca cagctcgttt ataaacgtct caccgctgag caggaatacg
     6181 aattaactgg cagcgaggaa acgttaaaag cggttattaa caccgactgg ggccagccgt
     6241 atttgccgcg cgcggcgtca gagcaacgcc gcagcgagga attaatgtcc cgcgccgagg
     6301 actacggcga tcgggttgta ccgcctgagg ttcgtttttt gctggcgtca gtcgatgtgc
     6361 aggcggggaa aaaacgccgt tttgttgcgc aggttgttgg ctacgggcag cacggcgagc
     6421 gctggttaat cgaccgtttt aatattgaat attcgctgcg ctgcgacgaa aacggcgagg
     6481 cgcgtcgcgt tgatcctggc ggctatccag aggactggga tctgctaata gatgaggtgc
     6541 tggaaaaatc ctatcagcta cagtccgacc cggtgcgccg gatgccgatt ttatgtctgg
     6601 cggttgatag tggcggcgag gacggcgtca cggataacgc gtataagttc tggcgtcgtt
     6661 gccgccgcga cggctacggg aagcgcgtgt atttaatcaa gggcgacagc acgacacgcg
     6721 cgaaattaat aactaaatcg tttcctgata acacgaaccg cagcgaccgc aaagctgggc
     6781 gcgtggtgac gtaccgatct acttgctgca aaccgacaga ctcaaagacg caatccacaa
     6841 caatctaaaa cgagacactc ccggccctgg ttttattcat tttcccgcct ggatcggtga
     6901 gtggttttac gacgaactgt tatatgaaac gcgtggatct gacggtaaat ggcgcaagcc
     6961 aggccacggc gctaatgagg cgttggacct gttttgctat tgccacacgc tggcaatat
//
LOCUS       DAHBHC010000152         6844 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHBHC010000152 DAHBHC010000000
VERSION     DAHBHC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.155"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            177..752
                     /locus_tag="M2A35_004804"
     CDS             177..752
                     /locus_tag="M2A35_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7200990.1"
                     /translation="MKQRKYLTQSEVELLLAEAGRSSTPERDSCLLYLSFIHGFRVSE
                     VCALRLTDVSLQDRSLHIRRMKNGFSTIHPIQRDEVRILKAWLRVRNSLPGAESEWLF
                     VSRQGAGLTRQRAWQVIKCLGERAGIEVSPHPHMLRHACGFALADRGVDTRLIQDYLG
                     HRNIRHTVIYTASNVERFRNIWSKKKIVTDS"
     gene            1236..1856
                     /locus_tag="M2A35_004805"
     CDS             1236..1856
                     /locus_tag="M2A35_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCB7200991.1"
                     /translation="MKKTIMSLAVVSALFSAGAMADLSADGKADNSASTATLNFTGKV
                     TSSLCQISSDDIAKTINLGEISAAQLKSGTGRGPSKSFEIKLVNCDASVSDISYVIRD
                     GSNSPDGNANTSNYLIPKLSETAAKGVGVYIVDPKDQPILIGNTKHNGVVKDGNNVPL
                     ASQTISLGAYIGTQTGAADEATNSQVEAGDVSAVGIMTIKASAQVG"
     gene            1932..2636
                     /locus_tag="M2A35_004806"
     CDS             1932..2636
                     /locus_tag="M2A35_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7200992.1"
                     /translation="MRIFSSAFILAGMLLCSYASAASFGPRESTLIFDGRQGSTSYRI
                     DNSDARLPWLVQAWVEDINEERTDAFTSVPLVFRVEPSSTFTVRIVKKGTVPEDRETL
                     YWAVSNSLPGGERSEQKTDGDKISAQLSLAYRFKVPMIYRPAALAGKPQQPESLQWHT
                     AAGGKMTVRNPTPYVVQLNYVSAGGKRYQGKGVSWFISPEKTVTVDVSAGAGSRIKYG
                     VVNDYGAVREYEGVVR"
     gene            2617..3060
                     /locus_tag="M2A35_004807"
     CDS             2617..3060
                     /locus_tag="M2A35_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7200993.1"
                     /translation="MKESSVNIFLLIILSFLFLFISAQGQTREPEKGVAVDPLKITVK
                     DSRVFYFTVYNDTDNEYVITSRVIREDGSGDTPFVLSPPVQPLNKRDDVKVGLIYLPE
                     KKGNSREKVYYLSVSFIPRQHQKPVGGKGVSVPLVLEQQIPVVTE"
     gene            3089..5596
                     /locus_tag="M2A35_004808"
     CDS             3089..5596
                     /locus_tag="M2A35_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCB7200994.1"
                     /translation="MRLRKKLLLPSLLVFISNRAVGGDYFDPSLLAAGIDGENIDLSV
                     FSHPGGGIEGEQEVSVQVNDNFYTRKTLHFRNSGDKGLLPDFPAGFFDDLLAPEYRPV
                     QKDTLLSSADFMSQTPYSSVTFNQGEGRVDIRVPQAFLGRAAQLRSSPDTWENGVPAL
                     LMDYSLTGNRNDSDSYSSRSLYASARLGLNVGPWRLRTSGNYSQYQSESRWWRSRTEN
                     TSFYSTWLERDISALRASLRIGEVSTGGMILDSVSFRGVRLYSNDDMLSSRLRNYSPT
                     VKGFARTQAVVIITQNGRQVYQTNVPAGPFELNDFYISGYSGDLVVTVRESDGSEHSF
                     IQPFSTLPEMKREGVSGFELSAGRYDNHGSEDYYNDPLFVYGAWSRGFGGGVTVFGET
                     LQAGKYQSAGIGSTLSLGRVGAVSADISVSRAEKYDDIHTGQSYGLKYSKSQVETGTT
                     VTLATYRYSTKDFYSFGDFVSRTEQARYVWENRLKNRMTLSLNQSLGSLGSVSLSASQ
                     QDYWTSGEVNRSFSLSHSFSWRDIYFSTTFSMDQMSGNRYGYDNNRQIDFYMSVPLSR
                     LLGREEPTSSSVSWSATRTERQLRQTATLSGSVPDTRMRYRVGGNWGNGGAAEGQSAS
                     LTWNGDFTTASLGYTRSGGYRTTDFSLSGAAVAWSGGVAFGSNSVTDNGAIVVNTSGV
                     SGIGTSAGGRTTWPGTALISSPQLYTENRIDLRTDGLPDDIVLAETSARTVPSRGAVV
                     VLDYTVYRGGQVVFTLKRPDGSALPFGSVVSLDGMPSGKENTGIVGDEGRVYLAGVPE
                     KGRLTVNSGGKQCHADFVLPQDRKKSGPVAEAVAVCR"
     gene            5587..6273
                     /locus_tag="M2A35_004809"
     CDS             5587..6273
                     /locus_tag="M2A35_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCB7200995.1"
                     /translation="MSLRYMVFIVLSCLCAGRTYAESAGQTPYPEKSRVIIRESEAVV
                     TTGNLTITNPGSRPWLMQAWLEDENGVREGHVYPEISRLEPVFSRRLLIAPSLSQWRE
                     GREGLNWLVVRLIPSVERDGRSRLTIPVVLRLKVYLRSRTSGAKQEVPQLTCERRQDG
                     QLMLHNGGLHYVTLTELKDGQGRLAEGPPLMVAPGGQQAAGSGMTAGTYEYGYVDDRG
                     IIDYGRVMCR"
     gene            6264..6815
                     /locus_tag="M2A35_004810"
     CDS             6264..6815
                     /locus_tag="M2A35_004810"
                     /inference="COORDINATES: protein motif:HMM:NF012635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCB7200996.1"
                     /translation="MSLRVWAVAGGVMLMWGIRGYAADSGAGASASDYREQSTDITFT
                     ASVKEPACQIEAPASVDFGEPRVITVRERGVERDFNIILKKCTQRIPKPKLIFAGNLI
                     SDDGMYLKNKNGTEEYATGVGIRLYYRGNNVSLKEGLALSSPLSPDVTTTLPFRARLG
                     KEGSGEVTAGKVEASVTLSMTYY"
BASE COUNT         1566 a         1723 c         1976 g         1579 t
ORIGIN      
        1 tctgctgccc ggcatgtgct cacggaagga cgggagtggg gatattaccg aaaaaggagg
       61 gataaaatca tatctttttt taacaaataa atgtcttatg ttgtttcttc cgcaccgtgc
      121 ggatggtcct gccataaaaa agtggcgtaa gtggcataat gtaagtgagg ttttaaatga
      181 agcagcgtaa atatctgaca cagtcagaag tggagcttct gcttgcagag gcaggcagaa
      241 gcagtacccc ggagcgtgac agttgtctgc tttatctgag ttttattcat ggattcaggg
      301 tgagtgaggt ctgcgccctg aggctgacgg atgtcagtct gcaggacagg agcctgcata
      361 taagacggat gaagaacgga ttcagcacaa tacacccgat acagcgcgat gaagtgcgta
      421 ttctgaaggc ctggctgcgt gtcagaaatt cgctgcccgg cgcggagagt gaatggttat
      481 ttgtttcccg gcagggagcg gggctgacgc gccagcgtgc ctggcaggtg ataaagtgcc
      541 tgggtgagcg tgcgggaata gaagtgagtc cgcaccctca tatgctgcgg catgcgtgtg
      601 gttttgcgct tgctgacagg ggggtggata cgcgtctgat acaggattat ctcggacaca
      661 ggaacatccg gcacaccgtg atatatacgg caagtaacgt ggagcggttc aggaatatct
      721 ggtcaaaaaa gaagattgtg acagacagtt aggaccaaac tgtaaaccat tctatttaat
      781 ttgttttccc tgtcaagttt tgtctttcac ttctccttat cctcatgaaa taaaaagaaa
      841 aataacccaa ctgcttttca ctcagcccgt ctgccttctg cagtgccagc gtaacccgcc
      901 tgaaagtctt cacgcacaca acagatttat attctgaaaa taacatcata acaagccgtt
      961 tgctgatgta aacacacgta aaacacaact gttcaggcat aaaaaaccac aaaaccgtga
     1021 gttttacata atggtcctaa ctgtaaaact ggatttgcag ttaaccagac gtgctggtct
     1081 gcaccgcagt cgccagcctg agagccacgg aggtgttgtg tcgcagggca tacacaggct
     1141 ggcatcctga atgtagtgga tggtgctttc tcaggcctgc agggcagaca gcatggagag
     1201 aaaaattatc tgattcttat tgataagagg acattatgaa aaagacgatt atgtctctgg
     1261 cggtggtttc tgctctgttc agtgccggcg ctatggctga tctgagtgca gatggtaagg
     1321 ctgataactc tgcaagtact gccacgctta actttaccgg taaggtgacc tccagtctgt
     1381 gccagatcag ctctgatgat attgcaaaaa ccattaactt aggggaaatc tccgctgctc
     1441 agttaaaatc tggtaccggg cgtgggccat ccaaatcctt tgaaattaaa cttgtaaact
     1501 gtgacgcatc tgtttctgat atttcatatg tgattcgtga tggtagtaat tcacctgacg
     1561 ggaatgctaa tacatcaaac tacctgattc ctaagctgag cgagaccgca gcaaaaggtg
     1621 taggtgtata tattgtggac cccaaagatc aaccaatcct gattggtaat accaagcata
     1681 atggagtggt taaagatgga aataatgtcc cgctcgctag ccagaccatc tctttggggg
     1741 cgtatattgg tacccaaaca ggtgcagccg atgaagccac aaacagccag gttgaggctg
     1801 gcgatgttag tgctgtaggt atcatgacta tcaaggcatc tgcccaggtt ggttaattaa
     1861 ccccggttcc tcatcttcac tattttcatg ctctcgggct gattcctgtc agcccggttt
     1921 cgggtctgtt tatgcgtatt ttctcttctg cttttattct tgcagggatg ttgctctgtt
     1981 catatgcaag tgcagcctct tttggccccc gcgaaagcac actcatcttc gacgggcggc
     2041 agggcagcac ctcataccgg attgataact ccgacgccag actaccctgg ctcgtccagg
     2101 catgggttga agatattaat gaagaacgca ctgatgcatt tacgtctgtg cccctggtct
     2161 tccgcgtgga accatcctcc actttcaccg tccgtatcgt gaaaaaaggc acggtccccg
     2221 aagaccggga aacactttac tgggctgtct ccaactccct ccccggcggc gaacgcagtg
     2281 aacagaaaac cgacggcgac aaaatcagtg cgcagctcag cctggcatac cgcttcaagg
     2341 tccccatgat ttaccgcccc gccgcgcttg ccggtaagcc ccagcagccc gaatcgctgc
     2401 agtggcatac cgccgcaggc ggaaaaatga ccgtccgcaa ccccacgcct tatgtggttc
     2461 agctcaatta cgtcagtgcc ggcggaaaac gttatcaggg taagggggtt tcatggttca
     2521 tctcgccgga aaaaacggta accgtggatg tcagtgcagg ggccggcagc cggattaaat
     2581 atggcgtcgt gaatgattac ggggcggtgc gcgaatatga aggagtcgtc cgttaatatt
     2641 tttctgctga ttattttgtc tttccttttt ttatttatat cagcacaggg gcagacccgt
     2701 gaacccgaaa aaggtgttgc cgtcgacccc ctgaaaatta ccgtgaaaga cagtcgtgtt
     2761 ttctatttca ctgtctataa cgacacagac aacgaatatg tcatcacatc acgggttatc
     2821 cgggaggatg gcagcgggga cacgcctttt gttctgagcc cccctgtcca gcctctgaat
     2881 aaacgggacg atgtgaaagt cggtctgatt tatctcccgg aaaaaaaagg gaacagcagg
     2941 gagaaggtgt attacctctc cgtctcattt atcccccggc agcatcagaa acctgtcggg
     3001 ggtaagggtg tttctgtgcc tcttgttctc gagcaacaga tacctgtggt gactgaataa
     3061 taaatacatc cgctactgac ggggtattat gcgactgaga aaaaaattat tattaccatc
     3121 tctgcttgtt tttatcagta accgtgccgt gggcggcgat tattttgacc cgtcactgct
     3181 ggccgccggt attgatggtg aaaatattga cctgtccgta ttctcacacc ccggtggcgg
     3241 gattgaaggt gagcaggagg tcagtgtcca ggtcaatgat aatttctaca cccgtaagac
     3301 gctgcatttc cgtaactccg gggataaggg gctgctgccc gattttcctg ccggcttttt
     3361 tgatgacctt cttgctccgg aataccgtcc cgtacagaag gatactctgt tatcttctgc
     3421 agatttcatg tctcagaccc cctacagcag tgtcaccttt aatcagggag aggggcgggt
     3481 tgatattcgt gtgcctcagg cattcctggg gcgtgcggca cagttacgct cctcgccgga
     3541 cacctgggaa aacggggtgc ctgccctcct gatggattac agcctgaccg gcaaccggaa
     3601 tgattctgac agctattcat cgcgcagcct gtacgccagt gcccggcttg gccttaacgt
     3661 cggtccctgg cgtctgagaa cgtcaggtaa ctacagccag tatcagagcg agtcacggtg
     3721 gtggcgcagc cggacggaga acaccagttt ctacagcaca tggcttgagc gggacatcag
     3781 cgccctgcgc gcctccctgc gtatcggtga agtctccacc ggcggcatga ttctggactc
     3841 cgtcagcttc cggggcgtcc gtctctacag caatgacgac atgctgagct cacgtctgcg
     3901 taactactcg ccgaccgtga agggctttgc acgcacgcag gctgtggtca tcatcacgca
     3961 gaacggacgt caggtctacc agaccaacgt ccctgcaggc ccctttgagc tcaatgactt
     4021 ctatatctcc ggctactccg gggacctggt ggtcacggtc agggagtccg acggcagtga
     4081 gcacagcttt atccagccat tttccacact gcctgaaatg aagcgtgagg gcgtgtcagg
     4141 atttgaactg tctgccgggc gttacgataa ccatggctcg gaagattatt acaacgaccc
     4201 gctgtttgtg tacggcgcat ggtcccgtgg attcggcgga ggcgtcaccg tctttggtga
     4261 aacactccag gccggaaagt atcagtcggc aggcatcggc agtacccttt cgctggggcg
     4321 cgtgggggct gtctctgccg acatttcagt atcccgtgcc gaaaagtatg acgacattca
     4381 taccggtcag tcctacggcc tgaaatattc caaaagccag gtggaaaccg gcacaacggt
     4441 gacgctggcc acctaccgtt attccacgaa ggatttctat tccttcggcg actttgtgtc
     4501 acggacggag caggcccgtt atgtatggga aaaccgtctg aaaaaccgga tgaccctgag
     4561 cctgaaccag tctcttggct cgctgggctc ggtctccctg agtgcatcac agcaggatta
     4621 ctggaccagc ggggaggtga accgctcgtt ctctctgtcc cacagcttca gctggcggga
     4681 tatttatttc agcaccacct tctccatgga ccagatgagc gggaaccgtt atggctacga
     4741 taataatcgc cagattgatt tctacatgag cgtgcccctg agccgtctgc tgggacgcga
     4801 agagccaacc tcatcgtccg tgtcatggag tgcaacccgc acggagcgcc agctgcgtca
     4861 gactgccacg ctgagtggca gcgtgcccga cacgcggatg cgttaccgcg tgggcggaaa
     4921 ctggggaaat ggcggtgccg ctgaaggaca gagcgcctcc ctgacctgga acggagactt
     4981 caccacagcc tctctgggct atacccgcag tggcggttac cggacaacgg atttcagcct
     5041 gtccggtgcg gcggtggcat ggtccggcgg tgtcgcgttc ggcagtaaca gcgtgacgga
     5101 caacggtgcc attgtggtga acaccagtgg tgtgtccggt atcggcacca gtgcgggcgg
     5161 ccggacaacc tggccgggaa cggcgctgat ttcctcacca cagttatata ccgaaaaccg
     5221 tattgacctg cgtactgacg gactgccgga cgatatcgta ctggcagaga cctccgcccg
     5281 taccgtgcct tcccgtggcg cagtggttgt tcttgattac accgtgtacc gcggtggtca
     5341 ggtggtgttc accctgaaac gccctgacgg cagtgccctg ccgttcggct ccgtggtctc
     5401 cctggacggc atgccgtcag gtaaggagaa caccggcatt gtgggggacg aggggcgggt
     5461 ttacctggcc ggggtgccgg agaaaggacg cctgacggtg aacagcggag gaaaacagtg
     5521 tcatgcggac tttgtcctgc cacaggacag gaaaaagtca ggaccggtgg cagaggcggt
     5581 ggcggtatgt cgctgagata catggttttt atcgtcctgt cctgcctgtg tgccggacgg
     5641 acatatgcgg agtccgcagg ccagacgccg tatccggaaa aaagccgggt gattatcagg
     5701 gagagtgagg cggtggtcac cacgggaaat ctgaccatca ccaatcccgg ctcccgcccc
     5761 tggctgatgc aggcctggct tgaggatgaa aacggcgtca gggaggggca tgtttatccg
     5821 gaaatctccc ggctggagcc ggtgttttcc cgtcgcctgc tcattgcgcc gtccctgtct
     5881 cagtggcgtg aaggtcgtga ggggctgaac tggctggttg tcaggctgat tccgtcggtg
     5941 gagcgtgacg gtcgcagccg gctgaccatt ccggtggtgc tgaggctgaa ggtgtatctg
     6001 cgttcacgca cgtccggggc taagcaggaa gtgccgcagc tcacctgtga acgccggcag
     6061 gacgggcagc tgatgctgca taacggtggc cttcattatg tgacgctgac ggaactgaag
     6121 gacggacagg gccggctggc agagggaccg ccgctgatgg tcgctcccgg ggggcagcag
     6181 gcggcgggaa gcggaatgac agccgggaca tacgaatacg gttatgtgga tgaccgggga
     6241 attattgatt acgggagggt gatgtgtcgt taagggtatg ggcagtggca gggggggtga
     6301 tgctgatgtg gggcatccgg gggtatgcgg ctgactcggg ggccggcgca tcggccagtg
     6361 actatcgtga acagtcaacg gacatcactt ttacggcatc agtaaaggag cccgcctgtc
     6421 agattgaagc gccggcttcg gtggattttg gtgaaccgag ggtgattacg gtgcgtgaac
     6481 gcggtgttga acgtgacttc aacatcatcc tgaaaaaatg cacgcagcga attccgaaac
     6541 cgaagctcat tttcgccggt aatttaatca gtgatgacgg tatgtatctg aaaaacaaaa
     6601 acgggacgga ggagtatgcc acgggagtgg gtattcgtct gtattaccgg gggaataatg
     6661 tcagccttaa ggaggggctg gcactgagtt cgccgctgag tccggatgtg accacgaccc
     6721 tgccattcag ggcacgtctt ggaaaagaag gcagtggaga ggttacggca ggaaaagtgg
     6781 aagccagcgt tacgttaagc atgacttatt actaaatacc ggaacaggaa gggtgaatca
     6841 gaat
//
LOCUS       DAHBHC010000153         6558 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHBHC010000153 DAHBHC010000000
VERSION     DAHBHC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.156"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..648
                     /locus_tag="M2A35_004811"
     CDS             <1..648
                     /locus_tag="M2A35_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCB7200997.1"
                     /translation="LGGYLNLVHTSSGLWADIVAQGTHHSMKASSDNNDFRARGWGWL
                     GSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGS
                     HNDMTFGEGTSSRDTLRDSAKHSVSELPVNWWVQPSVIRTVSSRGDMSMGTAAAGSNM
                     TFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            759..3143
                     /locus_tag="M2A35_004812"
     CDS             759..3143
                     /locus_tag="M2A35_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCB7200998.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLLDGKNKNGQL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLIRLQEKLATPAGQQVVQTLQQTGDTRELL
                     DILADDVWLQNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMQDQLQKASAVLAVMDYTQLNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVSAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEGDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNEVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATEKIAGRINARLEDEG
                     FTVKISFPAISQLQTRLAVKTNLSALMEERTETVTRRRRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVINMNKVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQEHLTERLQALNERVPELITDSKALREELETLL"
     gene            3140..4045
                     /locus_tag="M2A35_004813"
     CDS             3140..4045
                     /locus_tag="M2A35_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCB7200999.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHISTAG
                     GRTFFRRLKEGLTGEGAVRQNAINASFAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQIAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            4042..5112
                     /locus_tag="M2A35_004814"
     CDS             4042..5112
                     /locus_tag="M2A35_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCB7201000.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMLSGASIG
                     ALNGAIIAASPDLSEAAARLEGLWKHLGENQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGVLLTTLLRHISTINGFDNLMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREIQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNIVIVSHLSDGSLWDRRAFPDTTILELRPR
                     KRLKHAGDGGNSGGLLSFMSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALPRSETVL
                     QKSLEITEEADSALRNAMARIK"
     gene            5452..6270
                     /locus_tag="M2A35_004815"
     CDS             5452..6270
                     /locus_tag="M2A35_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCB7201001.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVFE
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            6362..>6558
                     /locus_tag="M2A35_004816"
     CDS             6362..>6558
                     /locus_tag="M2A35_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCB7201002.1"
                     /translation="MTTVLHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLC"
BASE COUNT         1649 a         1634 c         1847 g         1428 t
ORIGIN      
        1 ctgggcggat acctgaatct ggtacacaca tcctccggcc tgtgggctga cattgtggca
       61 cagggaaccc accacagcat gaaagcgtca tcggacaata acgacttccg cgcccggggc
      121 tggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caacctgatg
      181 ctggagccac aactgcagta tacctggcag ggactttccc tggatgacgg ccaggataac
      241 gccggttatg tgaaattcgg gcatggcagt gcacaacatg tacgtgccgg tttccgtctg
      301 ggcagccaca acgatatgac ctttggtgaa ggcacctcat cccgtgacac cctgcgcgac
      361 agtgcaaaac acagtgtgag tgaactgccg gtgaactggt gggtacagcc ttctgttatc
      421 cgcaccgtca gctcccgggg tgacatgagc atgggtacag ccgccgccgg cagtaacatg
      481 acgttctcac cgtcccggaa tggcacgtca ctggacctgc aggccggact ggaagcccgt
      541 gtccgggaaa atatcaccct gggcgttcag gccggttatg cccacagcgt cagcggcagc
      601 agcgctgaag gttataacgg tcaggccacg ctgaatatga ctttctgatt atacggtatc
      661 ccggaaatac catttcaggg aaattcatgc agtaatggga aaggatgctc attgctgttc
      721 accgcagttc tgaattattg aatttataaa ggtgaatgat gcacgaaaag aacatcgccc
      781 tgctttgtga tgaagccgac cggcttttgc aactgaatat caatctgctc cggcaaatgg
      841 ttgatgagcc ggatgtgtta ttggacggta agaacaaaaa cgggcagctt tttgataaac
      901 agaaagcact gaaaagaatt gaggagctgg agggcgaaca aatcaaaacc gcccgcaggg
      961 agatggtgct ggctgtggtc ggcacgatga aggcgggcaa atcaaccacc atcaacgcca
     1021 ttgtggggca ggaaattctg cccaaccgta accgccccat gacctcggtg ccgacattga
     1081 tccgccacgt tcccggaaaa acggaaccgg tcctccatct ggaacatatt cagcccgtcc
     1141 gcaatttatt aatcagactg caggagaaac tcgctacccc ggcaggacag caagtcgtac
     1201 agaccctgca gcaaaccggg gatacccgcg aactgctgga tattctggcg gatgatgtct
     1261 ggctccagaa tgaataccac ggggaagacg aaatctttac cggactggca tcgttaaacg
     1321 atctggttcg tcttgctgca gcaatgggga ctgaattccc ttttgacgaa tacgcagaag
     1381 tgcagaaact gccggtgatc gacgtggagt tcagccacct ggtgggaatg gatgcatgtc
     1441 agggaacact cacactgctg gatacccccg gccctaatga agccggacaa ccgcagatgg
     1501 aagtgatgat gcaggatcaa ctgcagaaag cctctgcggt gctggcggtg atggactaca
     1561 cccagttgaa ctcgaaagcc gatgaggagg ttcgtaaaga gcttaatgcc attgctgacg
     1621 tatcagccgg ccgcctgttt gtgctggtca ataaatttga tgaaaaagac cgcaatggcg
     1681 atggagcaga tgccgtacgc cagaaagtat cggcaatgct gaacagcgat gtgctgcccg
     1741 cctcccgcgt ttatcccggc tcatcacgtc aggcgtacct tgctaaccgt gcgcttcatg
     1801 agttacgaaa aaacggtgcg cttccggttg atgaagcctg ggtcgatgat tttatcaggg
     1861 aagctttcgg acctatggtg gaagagggcg actggaaaga cagcactaag gtaaataaaa
     1921 aggcagaaaa actctggaat atatctctga tcgatcaact gataacggaa gtcatacaga
     1981 gctcccattc cagagcggcg gcactggcgg ttgactctgc cgccgcaaaa ctgatgcaga
     2041 atgcagaaaa tgtcagcgaa tatctgtcat tacgtcatca ggggctgcag caaagcattc
     2101 agtcactgca ggcgcatatt accagcctgc tggcggatat ccaggagatt gaggaatgtc
     2161 agaatgaggt gaccggtgat gtcagaatgg ccatggagga catcaatacc aaaaccgggg
     2221 aattactgac gaaggtctgt gcctcactgg aggaggagct gaacgactat ttcagaagcg
     2281 gtaaacgcaa agaacagcaa atgctggagg aagaaaattc agcgcagccc cgggagagaa
     2341 acgcattcgc tttctttcac gatattttcg gaaccggaaa tcagcatgac aggatgcggg
     2401 actttgatcc ggacagcccc gaaataaaat tcagtgatcg cagggcagcc cttgaactga
     2461 tgacgcaaat cgaatcgacc gtgaccagcc tgcaccgtga ggctgaagca cagtttcggc
     2521 ccgaactgga aaaaatcgtc agggggattg aaacaggttt tcgtggtacg gccctgtatg
     2581 ccacagaaaa gatcgccgga cgcatcaatg cccgactgga ggatgagggc ttcactgtaa
     2641 aaatcagttt tccggcaatc agccagttac agacccggct cgcggtaaaa acaaatctga
     2701 gcgcgcttat ggaggaaaga acggagacag taacccgtcg ccgtcgacag gatggtgtct
     2761 ggggtacgct ttgtcgatgg gcaaacacca gtgactgggg ctggaaagag tacagtgtgg
     2821 atgtgagccg cagcgtgatc aatatgaaca aggtcaggaa ggaagtgatg tcactgaccc
     2881 gggcatattt cggggagttg caggcatcca ttgagcagaa tattaaccag cccgtccgcc
     2941 aggagatcga tgattttttc tgtacattca gggaaaaagt cgaacagcta cgtaacacac
     3001 tcattcagag ctcagaagat cataaacgcg atcagcaggc acaggagcat cttacggagc
     3061 gccttcaggc attaaatgaa agggttcccg agctgattac tgacagtaag gcgttaaggg
     3121 aagaactgga gacactgctg tgacctcacc atttattcaa caaattgctg ataaccgggt
     3181 atgtcaggta ctcacctgcc ttcctgaaaa atttgtggtt gattttgcaa acggtattga
     3241 tgttgcacag gagcatatca gcacggccgg ggggcgtacg ttcttccggc gtttaaaaga
     3301 aggtctgacc ggcgaaggcg ctgtacgcca gaatgccatc aatgcgtcgt ttgcacaggg
     3361 cgttgaggcg tccctgcgct ggctgacgga gctgacaacg tcgcttgcca ccacaaatta
     3421 cgcaattacc cgggtaaacg acagggtcag ttcactggtc agtgatactg ccaggctggc
     3481 gcattattcg gcagacacgc gggagcagtt actcaccctg gccgatcagg ttcaccagaa
     3541 actgaatcat cttgaagaaa aactccaccg tgttgaccag gttcagcggg cacagttaca
     3601 tcttgagcag atattctcat ggtggagcgc cgggcgatac gcgtcatttt cccctgccgg
     3661 acgttgttat gtggcgcttg aagagcttcg ctggggcgcg tttggtgatg tgatacgtca
     3721 gggtgaaaca ggccaggtta accagttact ggatattctc agacataaag cattaacgca
     3781 gatagcacag gagagtggcg gtagtgcaac cgtgcgtctg aacactctgg actggcttgg
     3841 tggtcagggc cgggagcagg cagataacga gtggcatgac gcgattaact ggctggggga
     3901 ctggtgcagt gaagagcggc atccggtgat ctggtcaacc acgcaggctg cagaacactt
     3961 accggttcgt atgccccgtc tctgctctgc agagcgcctc tctgaaagta tggttgatga
     4021 aatatttcag aagggggcgg catgagtaca gaaatgaaaa cggggctggt gctgtccggt
     4081 ggcggggcgg tgggcgctta tcaggcaggt gtggttaagg ctctggcaga gtgcggcaca
     4141 cagatcagta tgctctcagg agccagtatc ggggctctca atggtgccat tatcgcggcc
     4201 tctcccgatc tgtcagaagc cgccgcacgc ctggagggtc tctggaagca tctgggagag
     4261 aatcaggttc tgtcggtaaa ccgatcggtt tacttttcat tgctgaaaaa attgttccag
     4321 gcaatgaacc tctgccagat ccccggacgt gcaggagtac tgcttacgac gcttcttcgc
     4381 catatatcga caatcaacgg ttttgacaat ctgatgactc agccgttgtt gtcagatgag
     4441 cctctgacag cgctgatgga tcattatctt gatactgatg ctctggcaga cgggctaccg
     4501 ctgtatgtgt cgctgtaccc cacagaaggg ggcatgcagg atattattga ctgcattcgt
     4561 gctgaactgg gtgtcggaac cacgaaaaac gccgtttttc agcatatcca gagtctgccc
     4621 cgcgggcagc agaaagaagc cttactggcg tcagccgcgc tgcccctgct gttccgtccc
     4681 cgcgagattc agggaacaat gtacggtgat ggtgggatgg gcggatggcg aaacaggcag
     4741 gggaatactc ccgtgacgcc actggtggat gccggatgca atattgtgat tgtgtcgcat
     4801 ctgagtgacg gttctttatg ggatcgtcgg gcttttccgg acaccacgat ccttgagctc
     4861 cgtccccgga aaaggctgaa acatgcaggt gatggtggca acagcggcgg tctgctcagt
     4921 ttcatgtcgg cacataccga cgcctggcgt cagcagggct acgaggacac gatgctgacg
     4981 atggagcata tccggaaacc gctggcagca cgtcaggcac tgccccggtc agaaacggtg
     5041 ttgcagaaaa gtctagagat aacggaagag gcagattcgg cactgagaaa cgcgatggcc
     5101 cggattaaat aaagacgccc caaagaaacg ccaccgcctg tggtggtgct tttcctgtct
     5161 ggtgttattg aaaatatttt cagatatata ttatctgggt gatggttaag atgaatttat
     5221 ttaattttgg gggaagatac aaaaacaacc agtgaacata tttccggcgg gttatccgca
     5281 atgctgaagg tcagttgcac tggcgatgcc ggaatacagc ttaatacgtg gttcgccagt
     5341 gaaggacttc tcagacaata aacctcattc atttcaccca tcaggccgcg tcttctccgg
     5401 gagacgcggc tttttcattt ataccgctaa ctcattcata aggagcaaat aatgcgatta
     5461 gcgagtcgtt ttggtcatgt aaaccagata cgccgtgacc gtccgctgac acacgaagaa
     5521 ctgatgcgcc atgtaccgag tatttttggg gaaaaccgac atacctcccg aagtgaacac
     5581 tatgcgtaca ttcccactat caccgtcctg gaaaatctgc agcaggaagg ctttcagccg
     5641 ttctttgcct gccagacccg cgtgcgcgac cagagccgcc gggaatacac aaaacacatg
     5701 ctgcgtctgc ggcgggccgg acagataacc ggtcagcatg tccctgaaat tattctgctc
     5761 aactcccatg acggttcatc cagctaccag atgttacccg gatattttcg tgccatttgt
     5821 accaatggac tggtctgcgg tcagtctctg ggagaattgc gtgttccaca ccggggaaat
     5881 gtggtggaga aagttattga aggggcttac gaggtggtgg gcgtgtttga gcggattgag
     5941 gaaaagcgtg atgccatgca gtcgctgatt ctgccgccac cggcacgcca ggcgctggca
     6001 caggcggcac tgacttaccg ttatggtgac gaacatcagc ccgtcaccac cgccgacatt
     6061 ctgacaccac gacgccggga ggattacggt aaggacctgt ggagtactta tcagaccatt
     6121 caggagaata tgctgaaagg cggaatttcc ggtcgcagtg ccagaggaaa acgtatccat
     6181 acccgtgcca ttcacagcat cgacaccgac attaagctca accgcgcatt gtgggtgatg
     6241 gctgaaacgc tgctggagag tatgcgctga tgccgtttcc ctgaaagcgc agttctgact
     6301 cacgtctgtc ccttcccccg ggatttcttc cattcattta ctttttataa ggaataatct
     6361 catgacaacc gtcttgcata attccaccac tccttctgtt tccgtaaccg ctgcatcagg
     6421 gaatgaccag cctcagctgg ttgccaccat agtccctgat gaacagcgca tcagcttctg
     6481 gccgcagcac tttggcctca ttccacagtg ggtggccctg gagccccgtg tcttcggctg
     6541 gatggaccgt ctgtgtga
//
LOCUS       DAHBHC010000154         6385 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHBHC010000154 DAHBHC010000000
VERSION     DAHBHC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.158"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            188..1150
                     /gene="parM/stbA"
                     /locus_tag="M2A35_004817"
     CDS             188..1150
                     /gene="parM/stbA"
                     /locus_tag="M2A35_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCB7201003.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            1153..1503
                     /locus_tag="M2A35_004818"
     CDS             1153..1503
                     /locus_tag="M2A35_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCB7201004.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITQPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            complement(1654..2163)
                     /locus_tag="M2A35_004819"
                     /pseudo
     CDS             complement(1654..2163)
                     /locus_tag="M2A35_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2605..4140
                     /gene="cba"
                     /locus_tag="M2A35_004820"
     CDS             2605..4140
                     /gene="cba"
                     /locus_tag="M2A35_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HCB7201005.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVYSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYPGDKYKVLSREIAENINNFQGKTIRSYDDAMSSINKLMADSSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSLTLGSALIAFGLSATVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
     gene            complement(4158..4685)
                     /gene="cbi"
                     /locus_tag="M2A35_004821"
     CDS             complement(4158..4685)
                     /gene="cbi"
                     /locus_tag="M2A35_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HCB7201006.1"
                     /translation="MTSNKDKNKKANEILYAFSIIGIIPLMAILILRINNPYSQVLYY
                     LYNKVAFLPSITSLHDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQ
                     SCFWGAVFYAILIYITLFYNLELTTAGSFFKLLSHNVITLFILYCSIYFTVLTMTYAI
                     LLMPLLVIKYFKGRQ"
     gene            4929..5744
                     /gene="cma"
                     /locus_tag="M2A35_004822"
     CDS             4929..5744
                     /gene="cma"
                     /locus_tag="M2A35_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HCB7201007.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGVIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(5794..6147)
                     /gene="cmi"
                     /locus_tag="M2A35_004823"
     CDS             complement(5794..6147)
                     /gene="cmi"
                     /locus_tag="M2A35_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HCB7201008.1"
                     /translation="MKVISMKFIFILTIIALTAVFFWSEDKGPACYQVSDEQARTFVR
                     NDYLQRMKRWDNDVQLLGTEIPKITWENIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT         1984 a         1260 c         1306 g         1835 t
ORIGIN      
        1 ggcgacatct tttgctaccc gccaaaaaac ccaaaaacaa cccataacac ccccaataaa
       61 acaccaaaag aagacaaata atcattgatt gatggttaaa atggggtaaa cttgacaaat
      121 aaacccactt aaaacccaaa acacacccaa atacacgcca aaaaaaacac cataaggagt
      181 tttataaatg ttggtattca ttgatgacgg ttcaacaaac atcaaactac agtggcagga
      241 aagcgacgga acaattaaac agcacattag tccgaacagc ttcaaacgcg agtgggcagt
      301 ctcttttggt gataaaaagg tctttaacta cacactgaac ggcgaacagt attcatttga
      361 tccaatcagc ccggatgctg tagtcacaac caatatcgca tggcaataca gcgacgttaa
      421 tgtcgttgcg gtgcatcacg ccttactgac cagtggtctg ccggtaagcg aagtggatat
      481 tgtttgcaca cttcctctga cagagtatta cgacaggaat aaccaaccca atacggaaaa
      541 tattgagcgt aagaaagcaa acttccggaa aaaaattaca ttaaatggtg gggatacatt
      601 cacaataaaa gatgtaaaag tcatgcctga atctatacca gcaggttatg aagttctaca
      661 agaactggat gagttagatt ctttattaat tatagacctc gggggcacca cattagatat
      721 ttctcaggta atggggaaat tatcagggat cagtaaaata tacggagact catctcttgg
      781 tgtatctctg gtcacatctg cagtaaaaga tgcactttct cttgcgagaa caaaaggaag
      841 tagctatctt gctgacgata taattattca cagaaaagac aacaactatc tgaagcaacg
      901 aattaatgat gagaacaaaa tatcaatagt caccgaagca atgaatgaag cacttcgtaa
      961 acttgagcaa cgtgtattaa atacgctcaa tgaattttct ggttatacgc atgttatggt
     1021 tataggcggt ggcgcagaat taatatgcga tgcagtaaaa aaacacacac agattcgtga
     1081 tgaacgtttt ttcaaaacca ataactctca atatgattta gttaacggta tgtatctcat
     1141 aggtaattaa taatggacaa gcgcagaacc attgccttca aactaaatcc agatgtaaat
     1201 caaacagata aaattgtttg tgatacactg gacagtatcc cgcaagggga acgcagccgc
     1261 cttaaccggg ccgcactgac ggcaggtctg gccttataca gacaagaccc cagggcccct
     1321 ttccttttat gtgaactgct gacgaaagaa accacatttt cagatatcgt gaatatattg
     1381 agatcgctat ttccaaaaga gatggctgat tttaattctt caacaatcac tcaaccctct
     1441 tcacaacagg agcaaagaag tgatgaagag acaaaaaaaa acgcgatgaa gttaataaac
     1501 tgattaaatt tttattgata tctttacccc cataaacggg gtaaaggtat caacaaacct
     1561 attttttagc tatgtacaga ttacatctga caagttattt ctacgtaaca aatcacagtc
     1621 taaaaatcag tataacttta ccctttgcac tgttcagatt attcctttca gaaaaaaaac
     1681 gggaacatca gttactttat aaatctgcat aataagtagt attctatgcc tgccatctat
     1741 aattactttc ccgtcttcgg acatttgtgg atattcaatc gttccttgac ttaaataggt
     1801 tttaattttg tgccatcggc gtggatcgat attgaaagcc tgaacgccaa gcgaccttaa
     1861 cagggttaac ccattaatca tcgtgaccac aggcttttgt ctcattctct caggaagttg
     1921 atggcaatta gcagccaggg ccgccggggg aaaataaaac tccagttcct gtagtgaagg
     1981 gtattgcaac ccactgttgt ccagaggaat atcatcaaag gatttgaaag gatacttgga
     2041 catatacgca ttgatttttt caggcggtaa atgtgcaaac cagtcaggat caggcctgtt
     2101 cgcttcttct tctttaattc gttgaaatga ctacggaaac aaatcttcca cacttatgtt
     2161 catcacaata tcctcccttg agagcattgc tcatcgccac cgataaggat ttcattataa
     2221 aacacgccag tacagaacaa ctcactggtt tcaaactccc gtcgtccgtc ctgaccggta
     2281 cggtaactaa caaggcctgt acacatatca cgatgtgtac catgccataa agtgacagta
     2341 ttccccccaa gtgtctcata tcatagtttc attaaaacat gctgaggcca gagaacagtt
     2401 atgcctgtgc cgttttttgt ggtatcaggc gagaatagca cgtggtgtga atgcctgttt
     2461 tttgatcgtt ttcacaaaaa ttgaaatcca cagtcttgac agagaaaaca cagcggcgta
     2521 atttttgatc tgtacataaa accagtggtt atatgtacag tatttgattt taattgattg
     2581 tttttaaagt caaagaggtt ttctatgagt gataatgaag gtagtgtccc gacagaaggt
     2641 attgattacg gggacacaat ggttgtgtgg ccgtcaactg gacgaattcc gggcggtgat
     2701 gtgaaacccg gaggctcatc aggtctcgcc ccatccatgc ctccgggatg gggggattac
     2761 agcccacaag gtatcgcact tgtacaaagt gttctttttc ctggaattat tcgccggatt
     2821 attcttgata aggaacttga agagggagac tggtcgggat ggtctgtcag tgtgtatagc
     2881 ccctggggaa acgagaaagt ttccgctgca cgaacagttc ttgaaaatgg tttacgtggt
     2941 ggtttaccag aaccgtctcg cccggctgct gtttcttttg cccgtctgga gcctgcttcc
     3001 ggaaatgagc aaaaaattat tcgtcttatg gttacacagc aactggagca ggtaacggat
     3061 atccctgcca gccagttacc agcagcgggt aataatgtac cggtaaaata tcgtctgacg
     3121 gaccttatgc agaatggtac acaatatatg gctattatcg gaggtattcc gatgacagtg
     3181 ccagtagtgg atgccgttcc ggttccggac cggagtcgtc cgggaaccaa tattaaagat
     3241 gtttacagtg cccctgtatc accaaatcta ccggacctgg tattaagtgt gggtcagatg
     3301 aatactccag ttcggtctaa tcccgaaatc caggaagatg gcgttatttc tgagacaggg
     3361 aattatgttg aggctggtta tacgatgtcc agtaataatc atgatgtcat tgtccgtttt
     3421 cctgaaggca gtggagtttc tccgctatat atttcagccg tggagattct ggacagtaat
     3481 agtttaagcc agcgccagga agccgaaaat aacgcaaagg atgacttcag ggtcaagaaa
     3541 gaacaagaaa atgacgagaa gacggtcctg acaaaaacca gcgaggtcat cattagtgtc
     3601 ggtgacaaag tcggggaata tcctggagat aaatacaagg tgctttcccg tgaaattgca
     3661 gagaatataa ataattttca gggaaaaacg attcgtagtt atgatgatgc aatgtcttcc
     3721 attaataagt taatggctga ctccagcctt aaaataaatg caacggacaa agaagccatt
     3781 gtgaatgcgt ggaaagcatt taatgctgag gatatgggga ataaatttgc tgcgttgggt
     3841 aagacgttca aagcagcaga ttatgcaata aaggcgaaca acatcaggga gaagagtatt
     3901 gagggttacc agactggtaa ctgggggcca ttaatgctgg aagtcgagtc ctgggttatc
     3961 agtgggatgg catctgctgt agctcttagt ttgttttctt tgacattagg ctcggccctt
     4021 atagcctttg gtctttcggc cacagttgtt ggttttgttg gcgtagttat tgcaggtgct
     4081 attggtgcat ttatcgatga taaatttgtt gatgagttga atcacaagat cataaaataa
     4141 ttaaaagaaa ggcagtgtta ctgcctccct ttaaaatatt taatgacaag caatggcatc
     4201 agtaaaatcg catatgtcat ggttaaaaca gtaaagtaaa tggagcagta taaaataaac
     4261 agagtgatga cattatgaga caataattta aaaaaactac ctgctgttgt tagttccaga
     4321 ttatagaaca gtgtgatata aatcagaata gcataaaaaa cggcccccca gaaacaggat
     4381 tgcacaacaa gttttctggt tactggcttt atgatttctc ttgtcttata tgtgcaaaga
     4441 aaaacgagaa tccccataac tggtgctgtc ttgttgtagt ttgacataag tgttgtcatg
     4501 acgggatcat gcaatgatgt aatagaaggg agaaatgcta ccttattata taagtagtac
     4561 agcacttgag aatatggatt atttattcga agtattaata tagccattaa tggaataatc
     4621 ccgattatgg aaaatgcata taatatttcg tttgctttct tgttcttatc tttattgctg
     4681 gtcatatata tacctttttg tatttttata tatagattat atatcagagt tctcaatata
     4741 tttccagtat ttaatggagt gtatagatat atatcttata tatggtagtt caggtgcttc
     4801 cctaccattt ttaactgcat atccggcaaa caatgttatg gggagcctct ctcatggtgt
     4861 tcgttagccg gtgtaagcgt tagccagccg cgaactctgt tatcttatta acttataagg
     4921 agctatgtat ggaaacctta actgttcatg caccatcacc atcaactaac ttaccaagtt
     4981 atggcaatgg tgcattttct ctttcagcac cgcatgttcc tggtgctgga cctcttttag
     5041 tccaggttgt ttatagtttt ttccagagtc caaacatgtg tcttcaggct ttaactcaac
     5101 ttgaggatta catcaaaaaa catggggcca gcaaccctct cacattgcag atcatatcga
     5161 caaatattgg ttacttctgt aacgccgacc gaaatctggt tcttcaccct ggaataagcg
     5221 tttatgacgc ttaccacttc gcaaaaccag cgccaagtca atatgactat cgctcaatga
     5281 atatgaaaca aatgagcggt aatgtcacta caccaattgt ggcgcttgct cactatttat
     5341 ggggtaatgg cgctgaaagg agcgttaata tcgccaacat tggtcttaaa atttccccta
     5401 tgaaaattaa tcagataaaa gacattataa aatctggtgt agtaggtaca ttccctgttt
     5461 ctacaaagtt cacacatgcc actggtgatt ataatgttat taccggtgca tatcttggta
     5521 atatcacact gaaaacagaa ggtactttaa ctatctctgc caatggctcc tggacttaca
     5581 atggcgttgt tcgttcatat gatgataaat acgattttaa cgccagcact catcgtggcg
     5641 tcatcggaga gtcgctcaca aggctcgggg cgatgttttc tggtaaagag taccagatac
     5701 tccttcctgg tgaaattcac attaaagaaa gtggtaagcg ataatataag ggccgggatt
     5761 ccggcctttt tatgcagcct ttataacagt gtattagtca ttcgcatatt caacatatcc
     5821 ctcctcacaa tggtacatgc cataatacat tctcttaccc tccggacctt cagctttaaa
     5881 tgggacaaga agtgtttttt catcttcaac atctgttaaa cttctttcaa tattttccca
     5941 tgtaattttc gggatttctg taccaagaag ttgtacatcg ttgtcccagc gtttcattct
     6001 ttgcaggtaa tcatttctta caaacgttct ggcctgttca tcgctgacct gatagcatgc
     6061 cggaccttta tcttcagacc agaaaaaaac agcagtaaga gcaataatcg ttaaaataaa
     6121 aataaatttc atgctaatta ctttcataaa atctccacaa cttctgtcgt tcattcgata
     6181 taaaaaaaca ttacgaatat atccgtacaa tgtgagcata ctgtgaagtg gctttaacaa
     6241 ttatgaaata atacaagaga tcacatattg cagtggtctc ttttttatag aagaaatcac
     6301 aattcataat tgtgacttat tatgcatgtc tgtttttcag cattgtgacc gcatcggact
     6361 caggcatcga aaactgcacc cggtg
//
LOCUS       DAHBHC010000155         6270 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHBHC010000155 DAHBHC010000000
VERSION     DAHBHC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.159"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            334..978
                     /locus_tag="M2A35_004824"
     CDS             334..978
                     /locus_tag="M2A35_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCB7201009.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            1065..1373
                     /locus_tag="M2A35_004825"
     CDS             1065..1373
                     /locus_tag="M2A35_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCB7201010.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            1787..2767
                     /locus_tag="M2A35_004826"
     CDS             1787..2767
                     /locus_tag="M2A35_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCB7201011.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            2760..3176
                     /locus_tag="M2A35_004827"
     CDS             2760..3176
                     /locus_tag="M2A35_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCB7201012.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(3178..4452)
                     /locus_tag="M2A35_004828"
     CDS             complement(3178..4452)
                     /locus_tag="M2A35_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCB7201013.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(4452..4889)
                     /gene="umuD"
                     /locus_tag="M2A35_004829"
     CDS             complement(4452..4889)
                     /gene="umuD"
                     /locus_tag="M2A35_004829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCB7201014.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(4886..5134)
                     /locus_tag="M2A35_004830"
     CDS             complement(4886..5134)
                     /locus_tag="M2A35_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCB7201015.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5552..>6270
                     /locus_tag="M2A35_004831"
     CDS             5552..>6270
                     /locus_tag="M2A35_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCB7201016.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCT"
BASE COUNT         1539 a         1427 c         1556 g         1748 t
ORIGIN      
        1 gctgaaggtc ctgcagagtc tgatggggca taagtccatc agctcaacgg aggtctacac
       61 gaaggtgttt gcactggatg tggctgcacg gcaccgggtg cagttttcga tgcctgagtc
      121 cgatgcggtg tctatgctca aaagaattcc ataagctctg acttttttaa atccctttcg
      181 gatatctcat ggatatatta tcggtatttc atattctgtt gacatgcagg accggtatag
      241 agtaaaaaaa catctattga gtacctcttg gatatccgaa aggtgtctta tggggtcttt
      301 atgggtatta catgtggatt ctggagatac actatgcctg ttattgctaa tacgcatccc
      361 aaaggtggtg tgggtaagac aacttcatcc gtaaacattg ttggtgaaat gaagtctgat
      421 acagttgatc tggatactca taccgggctt tctatcattc tggggctgag acctgaagga
      481 aaagaaattt ccgtgaaagt acccaaaaca gtggatgagt taatcgaaat tatgactccc
      541 tacaagaaca gcgataagac gttacttatt gactgtgggg gatttgactc agatcttacc
      601 cgtactgcga ttgcctttgc agactgcgtt attgttcctt caaaagactc cctgactgaa
      661 cgtattggtt tgatgcattt tgatggagta ctggacgaaa tcagctctat catggggacc
      721 gatataactg ctcacctgta tctctgtaaa gttaatccta acaagaagaa attccctaag
      781 cttgatgcaa tcctgccatc gttcaaacat ctgaagctga tgaagagccg catttctgct
      841 cgtgcagaat ttgaggatgt cattgagact gggatgggaa tcactgagtc tgttcatggg
      901 cgctattcgg caggaggtaa ggaagttatt gccctgatcg aagaaatcaa tcatctgatt
      961 gaaaataaca aacagtaagg tgtgtctatt gggtatccat atagtatcct ttgggctttt
     1021 aatggatatc ttttaagtat ctaacaaaca tcctgggtta tctcatgagc aaagcgaaat
     1081 ttagtgcaca ggctgaaaaa ctgaaaaacc tggtggattc tgccagatta caaacttctt
     1141 cttctgccgt aacagcaaca gccccgtctc agccaaatga aggtgcagag gaagtttcag
     1201 gtcgtcccgt ggctgccagg acgaaggcta agaatcttaa ggccataccg ctgtcgtatt
     1261 ttgaagctca tgcggaactt aaagcgacca gtaaaacatc tctggatttt agctcatata
     1321 ttattgaggc tataagagag aagcttgaac gtgatggtgc aataggtcaa taaactatcg
     1381 gataccttaa gggtatctga tgtgtgtctt ttaaatgcgt tatggggatt cttcacgtac
     1441 ctaaagagtc cccattagac atctttctgt ttatggtgac atgattcgtc accgctttcc
     1501 tttcctctca gtttcaccat ttcattcctt ttcccttaac ctgaaccatg atgtccacga
     1561 tgtttttata ttgcatttat gtatcacata taaccttcat aatgtttttg tatctatgat
     1621 ttcctctggt cttaaagttg tgtgtacaat gtacatgcaa tttatcgtgt ttttatgttg
     1681 catttgtcct ttcaaaaaga ccgttttttc attataattg aatatacatt gcatgtatga
     1741 agtatgtttg ttttttctat ttgtttatga gcaacggaga ctggttatga aaatctttat
     1801 tgatgatggt tctaccaaca ttaaacttgc ctggttggag gatggtgacg tgaaaacgtt
     1861 aatcagccct aacagcttta aaccggaatg gtcttttagt ttactggatg atgctgctcc
     1921 ggctaattac gagatcgacg gcgagaagtt ttcatttgat ccattaagtg ctgatgctgt
     1981 cgtaacgact gaaacacgct atcagtacag tgatgtgaat gttgtggcca ttcagcatgc
     2041 gctacagcag accggcctga aagcgcagcc tgttgatgtc atcgtcacat tgccgatctc
     2101 tgaatacctt gacgccaaca atcagaaaaa taagcaaaac attgaaagaa agaaaaaaaa
     2161 tgtgatgcgt gaagtacggg tgcaggggag tgatgcattt gttattcgca gtgtttccgt
     2221 tcttcctgaa agtattcccg ctggtttcag tgttcttgcg gggcttgagg atgatgaatc
     2281 ccttttgatc gtagatctcg gtggtacaac tcttgatgtg tcgcatgttc gtagcaaaat
     2341 gacagggatt acaaaaacct ggtgtgatcc gaatatcggc gtgtccctga ttacatccgg
     2401 ggttaaggaa cagatggctg tacatgctaa tacaagagtg agctcattcc aggctgacaa
     2461 tatcattgtg caccgtaatg aacctgacta cctttcccgt cgcatttata atgctgaaca
     2521 gcgtgagtct atcattaatg ttatcaatga acggcagaag ttgcttatta aacgagttaa
     2581 tgatgtcatc agccgtttca ccgactatac acatgtaatg tgtgttggtg gtggggctga
     2641 aattgtggcc gaggctgtga agaacctgac taaggttcct gatgagcgct tttatctgag
     2701 ctcatccccg cagtttgatt tagtcatggg aatgataaaa atgaaaggtg gtgtgactaa
     2761 tgagtgatga aaataaatcc agacgctgct catttgaact tttcccggat gaacggacag
     2821 gagataagat agcagatgag ctcattgcaa atgaaaaact gaaggagcgg ggacgtttca
     2881 tgcgcgcgat gttagttact ggtgcggcgt ttgcagctat agataaacgt cttcctttgc
     2941 tgatatcgga gttactgaca gaaaatacga ctctggatga tattaataag gtgatttcca
     3001 gcgtaatacc aggtgctttt tcggttgaaa agaaattgct tgaattactg gaaaaacaat
     3061 ctggtctcca tacgtctgtg gattgcagta ctcctcttac agagcagagt ctgtcgagaa
     3121 atgacggtga agaccagacc agaagaaacg ctgaaaatat gttcggagat gactgaacta
     3181 acccagcctg gccaggggaa tatctcgcca tttagttgta taacactgac tcaacatttc
     3241 ccgtttcatt tgccattcag gggcggttcc tcgccccgca aaccacactt tcccttttcc
     3301 cgactggttc agttcatcga gtgttttcat taacttttca ctgtttttac ggggctggat
     3361 ttcatcaaat aatcccggct gcgctatacc cgatggtgtg aaatcagcca gcatgacacc
     3421 tgccttcata tagcggtacc cttcacgcca gacatggttt aaggctctgc atgcggcggc
     3481 aataatgtcc cggctgtcct gtgtgggcaa tggaagcttt tccacagcgg cattgctgta
     3541 acagggttct tttactgcaa agggtgatgt ccgtacaaat gtcgtcacct gccggcaata
     3601 ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca tactgaacaa cagcctggtg
     3661 catggcatct ttgtctgtga ttcgttcacc aaaactgcga ctacagacaa tctgctgttt
     3721 tgccggtggt gcttcttcca gggatatgca ggactcgccg ttgagttcgc gtaccgtacg
     3781 ctcaagaatg acgctgaagt ttttccggat gaatgccgtg ttagcctgcg ccagctgcag
     3841 tgctgtgtta atacccagcg cattcagctt ttccgtcagt ctgtgtccta ctccccagac
     3901 ctcaccaact ggctgcagcc ccagtagctt caagatccga ttacggtttt ctgccgtcag
     3961 cgcgaccact ccggaaaact gtggccattg ctttgttgcc cactgtgcac ttttagccag
     4021 cgtttttgta ggcgcaatgc ccacccccat ggtgagtcct gtccagctct ttacctgttc
     4081 cctgagctga tgaccaaaaa actccggaga gatgcaatga tttatccccc gcaaatcaat
     4141 gaacatttca tcaattgagt agggctcaac tgcgggagaa agcgactcca gaacagccat
     4201 aacccgttgg ctcatgctgt ggtacagcgc ataattgctg gaaaatacat gtattttctt
     4261 ctccaggcgc atttgtctca cctgaaacca gggctgcccc attctgatgc caagggcttt
     4321 tgcctccggg ctgcgcgcga tcacacagcc atcgttattg ctgagtacga tgaccggttc
     4381 gttgcgaagg tccgggcgga aaactttttc acatgaggcg tagaaactgt tgatatcagc
     4441 cagtgcaaac atcagcgtaa ctcccgcgtc ctgtgtatca cgtgagtgac aacaccaaaa
     4501 atacagatgt tttccggata cagtgtgcgg aactccgggc tgtctgaaac cggctccagt
     4561 gccgggcgtg ggcgcaacag cagtcgtttg acggtgaact caccgtcgat ctcagcgata
     4621 acgatgtccc cgtgttgtgg tttttcggcc ctgtccacta ccagcagatc accattctgc
     4681 acgccagcct ggttcatcga ttcaccgctg gcgcgcagaa agaaggtggc tgcaggtctg
     4741 ctgatgcaat agctgttcag atccagttcc tgctcagcat aatcagtggc aggcgaagga
     4801 aaaccggcct ggcaacgatc ggcaaacaac ggtcgcacgt aactgtcatc gcctgaaggg
     4861 tcagccggac ggtgataaac ggtactcatg ctgcaggcag ccagctgtcg tcctgccaga
     4921 tttcttccag cagagacagg atacgttctt tatccttgtc gttgcgggct cctgtgacag
     4981 atacagacgg ggcgctgccc tggtctatac gcagccagat ttccgggtag tgtggtttca
     5041 gacgttgttc gatttcattc tgtagcgcct gaagtgtgcc tgatttgagg ttttttgtgc
     5101 tctggcggtc gaaaagaatt tcaatgcgga tcatgttctc atctcccttt tcttttgctg
     5161 tatgtatata cagtattcta ataactgaat ccgcacacag tcaaatgtca ttgttgtgac
     5221 taaaaaacgc tcaattctct gagtgtgtgg ctgtatgcct gctgtgggct gctgtgtcca
     5281 gcttatccac agcattttgt gcacggttct gtggacaaaa tacctggtta cccaggccgt
     5341 gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgccg gcctcctcac
     5401 ccggtcacgt ttcgtcgttc ctcctccacg cgctgcggct tcggggccgc acctgcattc
     5461 gtatgcggtc gcccggtaac aggtgcggca cggcctgatg gaggccgcat gtgagaggag
     5521 aattcccatg ccaaactggt gctcgaatcg tatgtatttt tctggtgaac cggcacagat
     5581 cgctgagatt aaacgactgg ccagcggtgc agttacaccg ctttatcgcc gcgccacaaa
     5641 tgaaggtatt cagctgtttc tggccggaag tgccggactt ctgcagatca ctgaaaatat
     5701 ccgctctgaa cagtgccccg gagtgacggc tgccggacgt ggtgctgtat cgacggaaaa
     5761 tatcgcgttc acccgctggc tgacacacct gcagaacggt gtactgctgg atgagcaaaa
     5821 ctgcctgatg ctgcatgaac tctggctgca gagcggtact ggacagcgtc gctgggaagg
     5881 attaccggat gatgtcaggg aaaccatcac tgtgcatttc accgcaaaaa gaggtgactg
     5941 gtgtgacatc tggggtagcg aggatgtatc ggtgtggtgg aaccgtctgt gtgacaacgt
     6001 agtgccggaa aaaaccatgc cgtttgacct gctgacggtt ctgccgaccc gcctggatgt
     6061 tgaagtgaat ggctttaatg gtggtgttct gaacggtgtt ccttctgcat atcactggta
     6121 tacagaacgg tatggcgtga agtggccttg tgggtatgac ctgaatatca gtagtcaggg
     6181 agacaactgc attcaggtgg atttcgacac gccgtggtgt cagccggaaa gcgacgttgt
     6241 tgcagcatta agccgccgtt tcggctgcac
//
LOCUS       DAHBHC010000156         6251 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHBHC010000156 DAHBHC010000000
VERSION     DAHBHC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.160"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1052
                     /gene="ftsK"
                     /locus_tag="M2A35_004832"
     CDS             <1..1052
                     /gene="ftsK"
                     /locus_tag="M2A35_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCB7201017.1"
                     /translation="LAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDP
                     KMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKI
                     AEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIAR
                     LAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESL
                     LGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGA
                     GGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQ
                     GHNGNREVLAPPPFD"
     gene            1207..1818
                     /gene="lolA"
                     /locus_tag="M2A35_004833"
     CDS             1207..1818
                     /gene="lolA"
                     /locus_tag="M2A35_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCB7201018.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            1829..3172
                     /gene="rarA"
                     /locus_tag="M2A35_004834"
                     /gene_synonym="mgsA"
     CDS             1829..3172
                     /gene="rarA"
                     /locus_tag="M2A35_004834"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCB7201019.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3263..4555
                     /gene="serS"
                     /locus_tag="M2A35_004835"
     CDS             3263..4555
                     /gene="serS"
                     /locus_tag="M2A35_004835"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCB7201020.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(4789..5169)
                     /locus_tag="M2A35_004836"
     CDS             complement(4789..5169)
                     /locus_tag="M2A35_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HCB7201021.1"
                     /translation="MASKVSIHKDVEYQDIAKVYGKALENWLNTGVLPDRFGNEGQWE
                     DNARLCGSFVYKLHIRLPSEPPWKKSKAQIDRTSNIYLVYTRHWMDYDNIQIISIMAP
                     DAHEKAKTSFMAELERRAEEFQNS"
     gene            complement(5169..5795)
                     /locus_tag="M2A35_004837"
     CDS             complement(5169..5795)
                     /locus_tag="M2A35_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein
                     SufA"
                     /protein_id="HCB7201022.1"
                     /translation="MKQKSKSAAIAAIATALTASLAPVPAKSTILPTTVIESVTHARV
                     VSMLTFEHHDGQVFLPLEEAIEYINGLNDNMRAALNAVIEERQNRGKGALFADKTAKI
                     IKICEESLKQHSRVKEAVKIVMDPDNLAKMYPDSMEQRIAYRNKLVRFGRAIAQGEFI
                     ARDAINAIKRSTAPDKTTSLGNTLSDGNVKAMIIAEHKNMGLPAPEFS"
     gene            complement(5887..6174)
                     /locus_tag="M2A35_004838"
                     /pseudo
     CDS             complement(5887..6174)
                     /locus_tag="M2A35_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1515 a         1477 c         1661 g         1598 t
ORIGIN      
        1 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
       61 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
      121 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
      181 cagaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
      241 tggagcgtcg gtataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
      301 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
      361 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
      421 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
      481 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
      541 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
      601 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
      661 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
      721 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
      781 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
      841 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
      901 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
      961 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
     1021 gtgaagtgct ggccccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
     1081 gcgtcgcatc cggcacttta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
     1141 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
     1201 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
     1261 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
     1321 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
     1381 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
     1441 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
     1501 gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
     1561 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
     1621 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
     1681 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
     1741 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
     1801 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
     1861 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
     1921 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatttaca
     1981 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
     2041 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
     2101 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
     2161 tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc cacatattga
     2221 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
     2281 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgagtacag aggatattga
     2341 gcaagtacta actcaggcga tggaagacaa aacgcgtggc tatggtggtc aggatattgt
     2401 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
     2461 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
     2521 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
     2581 aggcgatcgc ttttacgatc tgatttccgc actgcataag tcggtacgtg gtagcgcacc
     2641 cgatgcggcg ctgtactggt atgcgcgaat tattaccgcc ggtggcgatc cgttatatgt
     2701 cgcgcgtcgc tgtctggcga tagcgtctga agatgtgggt aatgctgatc cacgagcgat
     2761 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
     2821 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
     2881 tgcgtttaaa gccgcgctgg cggatgctcg cgaacgtccg gattatgacg tgccggttca
     2941 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
     3001 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
     3061 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
     3121 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
     3181 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
     3241 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
     3301 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
     3361 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
     3421 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
     3481 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
     3541 ggctgaaatt cgcgatatcg cgctaaccat ccctaacttg cctgcagatg aagtgccggt
     3601 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggcaccccgc gtgagtttga
     3661 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
     3721 cgcagttaag ctgacaggtt cgcgctttgt ggtgatgaaa gggcagattg ctcgcatgca
     3781 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
     3841 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
     3901 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
     3961 gctgatccca acggcagaag ttccgctgac caacctggta cgcggtgaaa tcattgatga
     4021 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
     4081 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
     4141 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
     4201 aaaagtcctg caactgctgg gcctaccgta ccgtaaaatc atcctttgta ccggcgacat
     4261 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
     4321 ccgcgagata tcttcctgct ccaacgtttg ggatttccag gcgcgtcgta tgcaggctcg
     4381 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
     4441 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
     4501 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
     4561 aatttttctg aatctaaaaa gcgcctgcgg gcgctttaag aagaagttta actatttgaa
     4621 aataaatgga ttttattttt aagtgtccat ataacgtcca tctttgaatg caaaaagccc
     4681 acaaattcta tgctgtgggc tttgtttttg gtgtcctcat gagtgaggga accaaaaaga
     4741 aaacccgcag tttttacgct gcgggtttct gggagaaaat caggcaattt atgagttctg
     4801 gaattcttct gcccgtcttt ccagctcagc catgaaagaa gttttggctt tttcgtgagc
     4861 atctggggcc atgatgctga tgatttgtat attgtcataa tccatccagt gccttgtata
     4921 aaccagatag atatttgaag ttctgtctat ttgggctttg cttttcttcc atggtggttc
     4981 tgacggtagt cttatgtgta atttatatac aaacgaacca cataatcttg cgttatcttc
     5041 ccactgccct tcattgccga atctgtcagg gagaacaccg gtatttaacc agttttccag
     5101 agcctttccg taaacttttg caatgtcctg gtattcaaca tctttatgaa tgcttacttt
     5161 gcttgccatc aggaaaactc cggtgccggg agtcccatgt ttttatgctc ggcaataatc
     5221 attgctttca catttccgtc agacagcgta ttgcctaggc tggtcgtttt gtctggtgcg
     5281 gtgcttcgtt tgatggcgtt gatggcatca cgagcgataa actctccctg cgcaattgcc
     5341 cgaccaaaac gaaccagttt attgcgatag gcgattcttt gctccatgct atccggatac
     5401 atttttgcca ggttatccgg atccattaca attttgacag cctctttaac acggctgtgc
     5461 tgcttaagtg actcttcgca tatttttatg atttttgcgg ttttgtcagc aaacagagct
     5521 cccttgcctc tgttctgtcg ttcttcaatg actgcattga gcgcggcacg catgttgtca
     5581 ttcagcccgt tgatatattc aatagcttct tcaagaggca gaaacacctg accatcatga
     5641 tgctcaaatg taagcattga aaccactctg gcgtgtgtca cggattcaat aactgttgtt
     5701 ggtagtatcg tgcttttagc tggcaccgga gccagtgatg ctgtcagagc tgtagcaata
     5761 gcagcaatgg cagcagactt tgatttttgt ttcataatgc actccacagg ttgatgtgtt
     5821 cagcatatcg caaatagcag cttgtataaa gaaacgccac atcagcagtg atcgttttac
     5881 gcgcttttct tcttggattt gctggtcggt ttttctgcgc cagcttccgt cagtcgtgac
     5941 atgctgaaac acttctgcac gctgttgttg caattgttcg ccgggcttta catcaggcag
     6001 gttatgaccg atgggtaaga tttgcctgat aacaggctta tccgggcgtc cttcaatgtt
     6061 ggatatttcc acgatggtgc ctgctggcgg gtaggcaaaa catcccgcct cactgccagc
     6121 tatggggcgg gggagtggta ctgccgggta aatgggtgta tcgctttttt tgttactccg
     6181 gcaattctgt ctgttaattc tgaggtaaag gcaaactcat ctgattgttt cccctgtgtg
     6241 aagctggcag a
//
LOCUS       DAHBHC010000157         6219 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHBHC010000157 DAHBHC010000000
VERSION     DAHBHC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.161"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..249
                     /locus_tag="M2A35_004839"
                     /pseudo
     CDS             1..249
                     /locus_tag="M2A35_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
     gene            241..435
                     /locus_tag="M2A35_004840"
     CDS             241..435
                     /locus_tag="M2A35_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCB7201023.1"
                     /translation="MAEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQMLKQL
                     GNLNPQQFMSGSQGGGNDGK"
     gene            425..604
                     /locus_tag="M2A35_004841"
                     /pseudo
     CDS             425..604
                     /locus_tag="M2A35_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
     gene            complement(591..989)
                     /locus_tag="M2A35_004842"
                     /pseudo
     CDS             complement(591..989)
                     /locus_tag="M2A35_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1368..1643
                     /locus_tag="M2A35_004843"
     CDS             1368..1643
                     /locus_tag="M2A35_004843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201024.1"
                     /translation="MTHALAQAHANAQKLKARQAEMQRKEDEERERERRQQNAKHSAP
                     ATDEPEYVELPRIPELSPEEQAALDKSMEKERAEMEKILGPIPTSFN"
     gene            1855..5952
                     /locus_tag="M2A35_004844"
     CDS             1855..5952
                     /locus_tag="M2A35_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCB7201025.1"
                     /translation="MNKIYSHRICHKTGIIKVVSELISHTRKSKSKKYDRTLFLIPVI
                     FSLLSFPTGSKASVVNGNNAYQNYRDFAENKGAFQPGATDIPVYDTNGNVTGTLSISM
                     PDFSSVATEGYATLISPQYIVSVKHNKGYNTVSFGDGQNSYTIVNRNEDPSRDFHTPR
                     LNKLVTEVSPADAISTGDSSNFYTDSERYTAFVRLGSGRQFIKNEDGTLTQLAAGYSY
                     LTGGTLKAPVVYQNGNSLKATTPDLFNSDMGLLPTTAQPGDSGSPLFAWDSWTGKWVL
                     IGVQTAISTSWSYWAAIPVSDIQAIINDDSDGTISFLDSAGALIWTYNSTDGTGTISQ
                     GNTTYTMHGTKDGNLDAGKNLTFTGDDGLILLRDNVDQGAGTLTFNSNYTVSTDNNST
                     WMGGGLDIAEGTSVTWQVNGVSGDNLHKIGAGTLIVNASGINEGGLKVGDGTTVLDQQ
                     PDKDGNVQAFSSVNIASGRATVVLADSNQVDPDNISWGYKGGTLDLNGNNLTFHQIKA
                     ADYGAVIANTADSTSTVTLSYSLNSNDVNINNWAGLNSKGTPGDLYVYNNTYTNTTDY
                     FILKTDGYYGYYPTNQSSSDIWEYVGHDQDAAQKLVADRYNSSGYLYHGQFTGNLNIV
                     NTVASGATGSFVMDGSADISGSFTQENGRLTLQGHPVIHAYNSQSIADTVASTGDDSV
                     LTQPTSFTQSDWENRSFTFGALNLKNTDFSLGRNATLNTVIQAENSVVTLGGARVYID
                     LKDGDGTDFELEEGESIATADADKSHFYGSVILDSNSTLTVNEVFNGNIQASDSSVTI
                     NSDEVLIGDVVLQNSAMTLNDGASAVVSDSLNTDGAVSVDNAFLQLGENGNTRTRSSA
                     GVYRAAQINLSGEKAALYVTEGNTLYSDILSDSSASLIFGDSTQALSGTSDGTQVTRW
                     EGSLNAEDASLTLYGTEWRVTSDSQSGTLSARNSLIDLSSYSDGSRRLSVSNFDSSDS
                     TYRLNVGSGYNDQLVVTESATGQNNTLLLNFTSQPQTGDVKQTLVIAPDSTSSDLFHV
                     STISNGFSEFIPETTVDTTDGVKKWELESIQVQENKPVTQAAGYFLRSGYQQFLTEMN
                     NLTIHMSDLRDNDAPTGVWARLINGSGSGKGDYSDRYTHFQMGADRKHELESMDLIHG
                     VMLTYTDSRADGKQYTGKTRSYGAGIYASGIFPDGAYFDLTGKYVQHANDYTLSLAGA
                     GQQTFHSHSLYAALQVGQRYRYGGNVWLEPQAELVYGRISGRNFSWSDNSIPLRMKSS
                     DMTPLVGRIGIESGKDIDMKKWSARLRAGVSYQADLRDHATVLLQDSASTYKSTSGKD
                     NRVLLNAGLQMNLGQDKRLDMGLEKSFMGDYNVDHAVKASFTWSF"
BASE COUNT         1702 a         1499 c         1523 g         1495 t
ORIGIN      
        1 gaacgtggtc tgaatctttc cattcaggcc gttccccgtg aaggcgcggg gcgtaccatt
       61 cagctggtca gtgacctgcg cggaaccgga gaagaagccg gtgcgtggga aacgtccacg
      121 ccttacgaat ccctgcttgt gaccatcagc caggctgtcc gtggcggaaa attacctgca
      181 ggctggtatc aggttcctgt gacaaaggaa accctgaagg cccctgcggg tctgtcttca
      241 gtggcagaga tggcgctgga gcgcacgcag aacagagcgg aagaagataa tccggaaagt
      301 ccggttcctg ttccgccatc agctgaaagt catcttaacg gctttaatac tgaccagatg
      361 ctgaagcagc tgggcaacct gaatccgcag cagtttatgt ccggcagcca gggaggaggc
      421 aacgatggca aataatatgt cgtcccgtca ggcgtgccat gccgcccggt atgttgtcgc
      481 ccgggttctg cgaggactgt tctggtgtct gaagtacacc gtcattctgc cactggccac
      541 aatggccctg atggctctgt ttgttttatg gaaggacaac accacgccgg ggaaattact
      601 ggttgtggtg gtgctgaaat ggcgctttta tccggcacag gaatactctt tttatttttt
      661 cgaatgaaag catcttcgcc atattttcta tatttttcaa gccagttgca tacagtacca
      721 aaggcaggta ttccgtgttt taccgctact tcacgaacag aaaaacagtc atccacgact
      781 tcttttacta ctgtgagttt taactcagca gaatagcgtc cttttttacc cgtaagtatg
      841 cttatcccat gttcccgata ggcataagac cattttctta ctgtgcaggg actgataccg
      901 tacatccttg ctacagattt cagactcagc tctccctcca ggtacttttc tgatactacc
      961 tttttgaact ccaatgaaaa cttttccatg catcctcagt ttttggtctg gtgtctaacc
     1021 tttggggcgc acttcaaaaa tgatggacat tatttttgtg gagccggagg aaacagacca
     1081 gacggtttaa atgagccggt tacgaaaaaa caaccgctac tctggataat gatgaccacc
     1141 gcactgcagt ccggctgcac aggcaacagg gaccatcacc cgcaggtgac attcacggtt
     1201 gataccgtgc gaacggacac gacggccagc aatcctgtat gcctgccgga aaaatacggg
     1261 agccttctta atgagcgcca tccggacctg tttgccggtg ctcaggggat tattacaaag
     1321 ggggtgccta taatgcagac agcagaacat ctacacctgc acgcccaatg actcatgccc
     1381 tcgcacaggc ccacgccaac gcgcaaaaac ttaaagccag gcaggcggaa atgcagcgca
     1441 aggaagatga ggagcgcgag cgtgaacgcc ggcagcagaa tgcaaaacat tcagcaccgg
     1501 caacggatga accggaatac gttgaactgc cacgaatccc tgaactttcg ccggaagagc
     1561 aggcagcact ggacaaatcg atggaaaaag agcgcgcgga gatggagaaa attctgggac
     1621 caatccccac ctccttcaat taagactctc cacctgccgt ctctctcagg gcaccgtatg
     1681 gtgccttgaa tgtttttccc taacgtcaga gaacgccctt cccctgcacc tgagcggtta
     1741 agtgcaccag acttaccttt gtattgattt tcattgatta atcaatagaa taagcttccc
     1801 ccagggaagt cacctcctgt acttttttat tatgcacata actacacttc agatatgaat
     1861 aagatatata gtcaccgtat ttgccacaag accggtatta ttaaagtcgt ttcagaactt
     1921 atatctcata cgcgaaaatc aaaaagcaaa aaatacgaca ggacattatt tttaattcct
     1981 gtcattttca gtctattatc ctttccgaca gggagtaaag cgtccgttgt aaacggaaat
     2041 aatgcatatc agaactacag ggatttcgct gaaaataagg gggcctttca gcccggagca
     2101 actgacatcc ctgtttatga cacaaatggc aacgtgactg gcaccctttc catctcaatg
     2161 cctgatttca gtagtgttgc gacagaagga tatgccacgc tgatttcccc acaatatatt
     2221 gtcagcgtta agcataacaa aggatacaat acggtaagtt ttggcgacgg tcagaactca
     2281 tacacgattg tcaatcgcaa cgaagatcca tccagagatt ttcatacgcc acgactgaat
     2341 aaactggtta ccgaggtttc tcccgccgac gctatttcaa cgggagacag cagcaatttt
     2401 tataccgatt ctgaaaggta tacagccttc gttcgcctcg gctccggtcg gcaatttatc
     2461 aaaaatgagg acggtacact gacacagctg gctgcagggt acagttatct gaccggggga
     2521 accctgaaag caccggtggt gtatcagaat ggtaactctc tcaaagcaac cacccctgat
     2581 ttattcaatt ccgatatggg actgttaccc acaacagccc agcccggtga cagcggctca
     2641 ccgttatttg catgggacag ctggacagga aaatgggtcc tcattggtgt gcagacggcc
     2701 atcagtacaa gctggtcgta ctgggcggcg atccccgtat ctgatattca ggccattata
     2761 aatgacgact ccgatggtac gatttccttt cttgactctg caggtgcgct gatatggaca
     2821 tataacagca ctgacggcac tggcacgatt tcacagggca atacgaccta taccatgcat
     2881 ggaacgaaag acggcaatct cgatgcgggt aaaaacctta catttaccgg agacgatggc
     2941 ctaatcctgc tcagggataa tgtggatcag ggtgccggca cgctgacatt taacagtaac
     3001 tacacggtga gcactgacaa taattccaca tggatgggag gcggtcttga tatcgctgag
     3061 ggcacctccg tcacctggca ggtcaatggt gtcagcggtg ataaccttca taaaatcgga
     3121 gcgggaacat taattgtcaa tgcttcaggg ataaatgaag gcggccttaa agtgggcgat
     3181 ggcaccaccg tacttgatca acaacctgac aaggacggaa atgttcaggc attcagtagt
     3241 gtcaatatcg caagtggcag ggcaactgtt gtgcttgcgg acagtaatca ggttgatcct
     3301 gataatatct catgggggta caagggagga acgctggatc tcaacgggaa taacctgaca
     3361 tttcatcaga taaaagctgc tgactatggt gccgttatcg caaatactgc agacagtaca
     3421 tccacagtga ctctgagtta ttctctgaac agcaatgatg taaatattaa taactgggcc
     3481 ggcctgaaca gtaaaggaac ccccggtgat ttgtatgttt ataacaacac ttatactaat
     3541 acgacagact atttcattct gaaaactgac ggatattacg gctattaccc gacgaaccag
     3601 agcagctctg atatatggga gtatgtggga cacgaccagg atgcagcgca gaaactggtt
     3661 gcagacagat ataattcatc cggttatctt tatcatgggc agtttaccgg gaatttaaat
     3721 attgttaaca ccgttgcatc cggagcaacc ggttcattcg tgatggatgg ctctgctgac
     3781 atctcaggct cttttactca ggaaaatggt cggctgaccc tgcagggaca cccggtgatc
     3841 catgcttata acagccagag tatagctgat acggttgcct ctaccggaga cgactccgtc
     3901 ctgactcagc cgacatcatt cacacagagt gactgggaaa accggagttt tacgtttgga
     3961 gccctgaatc tgaaaaatac agatttcagt ctggggcgta atgccacact gaataccgtt
     4021 atccaggctg aaaactcagt ggtcactctg ggtggcgcac gtgtttatat tgatttaaaa
     4081 gatggcgatg ggactgactt cgagcttgaa gagggcgaat ccattgccac ggcagacgca
     4141 gacaagagtc atttctatgg ttctgtcatt cttgacagta actccaccct gacggtcaat
     4201 gaagttttta acggcaatat tcaagcttca gacagtagtg tcacgataaa ctccgatgag
     4261 gtacttattg gtgacgttgt attacagaac tctgcaatga ccctgaatga tggtgcatcc
     4321 gcagtggtct ccgacagtct gaacactgat ggtgctgttt ctgttgataa tgcctttctg
     4381 caactcggcg aaaacggcaa tacccggacc cgttcttcag ccggtgtata cagggcagct
     4441 cagattaatc tgtcaggaga aaaggccgca ctgtacgtca cggaaggaaa cacgttgtac
     4501 agtgatattc tgtcagacag ctctgcatct ctcatttttg gagacagcac acaggcatta
     4561 tccggcacct ctgatggcac tcaggtaacc cggtgggaag gctcactgaa tgctgaggat
     4621 gccagcctaa cgctctacgg aaccgaatgg cgcgtgacca gtgattctca gtctggaacc
     4681 ctgtcagcga ggaactcgct gattgacctg tcatcttact ctgacggaag cagacgtctt
     4741 tctgtcagta atttcgacag cagtgacagc acataccgcc ttaatgtggg aagcggatat
     4801 aacgatcagc ttgtggtgac agaaagcgcc acgggtcaga ataatacact gttgctgaac
     4861 ttcacttcac aaccccagac gggagatgta aagcagacgc tggtaatagc gccagacagc
     4921 acatcatcag acctttttca tgtgtctacc atcagcaatg gtttcagcga attcatacct
     4981 gaaaccacgg tcgacacaac tgatggtgtg aaaaaatggg aactcgaaag tatacaggtg
     5041 caggaaaata aacccgtcac tcaggctgca ggttattttc tgaggagtgg ttatcagcag
     5101 tttctgacag aaatgaataa cctgacaata catatgagcg atctcagaga taatgatgct
     5161 ccgaccggcg tctgggcccg gctgattaat ggaagcggct caggcaaagg ggactacagc
     5221 gatcgctata cccatttcca gatgggggca gacaggaaac atgaactaga gagcatggat
     5281 cttattcatg gtgttatgct gacatataca gacagccgtg ctgatggaaa acaatatacc
     5341 gggaagaccc gttcatatgg tgccggtatc tatgccagcg ggatatttcc ggatggcgca
     5401 tactttgacc tcaccggaaa atatgttcag catgcgaatg actatacctt atccctggcc
     5461 ggggcggggc aacagacatt ccacagccat tcattgtacg ctgcattaca ggtaggacaa
     5521 cgttatcgct atggtggaaa tgtctggctt gagcctcagg ctgaactggt ttatggcagg
     5581 atctccggca gaaacttcag ttggagtgat aacagcatcc ccctaaggat gaaaagctct
     5641 gacatgactc ctctggtcgg acgaatcggt attgaaagcg gcaaggacat tgacatgaaa
     5701 aaatggtctg ctcggttacg agcaggtgtc agttaccagg ccgatttacg cgatcatgcc
     5761 acagtactgc tacaggatag cgccagtaca tataaatcca cctcaggcaa agataaccgg
     5821 gtgttgctga atgcaggttt acagatgaat ctgggtcagg acaaacgcct ggatatggga
     5881 ctggagaaat catttatggg tgactataac gttgatcatg ctgtaaaagc atcttttacc
     5941 tggtcattct gacgatacag ctccggaagg acagaagagc gctgaagcct ctgtcatcac
     6001 tctcagggca ccgcctggtg ccctctgcaa ttattaaagg gcaggaagcg gtcacctcct
     6061 gcccctggtt aaaaaaagag gtttcaaata tcaacgtaac cggctcattt aaaccgtctg
     6121 gtctgtttcc tccggctcca caaaaataat gtccatcatt ttttgaagtg cgccccaaag
     6181 gttagacacc agaccaaaaa ctgaggatgc atggaaaag
//
LOCUS       DAHBHC010000158         6167 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHBHC010000158 DAHBHC010000000
VERSION     DAHBHC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.162"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(104..1318)
                     /gene="ydcO"
                     /locus_tag="M2A35_004845"
     CDS             complement(104..1318)
                     /gene="ydcO"
                     /locus_tag="M2A35_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCB7201026.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIFTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1371..1907
                     /gene="sutR"
                     /locus_tag="M2A35_004846"
     CDS             1371..1907
                     /gene="sutR"
                     /locus_tag="M2A35_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCB7201027.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1980..3941
                     /gene="rlhA"
                     /locus_tag="M2A35_004847"
     CDS             1980..3941
                     /gene="rlhA"
                     /locus_tag="M2A35_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCB7201028.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4033..4263)
                     /locus_tag="M2A35_004848"
     CDS             complement(4033..4263)
                     /locus_tag="M2A35_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCB7201029.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNMDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4485..4639
                     /locus_tag="M2A35_004849"
                     /pseudo
     CDS             4485..4639
                     /locus_tag="M2A35_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4685..5101
                     /gene="hicB"
                     /locus_tag="M2A35_004850"
     CDS             4685..5101
                     /gene="hicB"
                     /locus_tag="M2A35_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCB7201030.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5180..>6167
                     /locus_tag="M2A35_004851"
     CDS             5180..>6167
                     /locus_tag="M2A35_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCB7201031.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHA"
BASE COUNT         1598 a         1554 c         1548 g         1467 t
ORIGIN      
        1 gatggtgcag tcaggccgcc cgttgaagca ggaacccacc gaaatgattc aggcgacagc
       61 ctgaacgtag cagggatcca cgtccttcag ggcgtggagg atgtcaatat cggtttctgt
      121 cagcgattaa attcaacacc acgtaacaaa cgcctccggc aattaatccc cagaacgcag
      181 aaccaatccc gaccagcgtc aatccacttg ccgttaccag aaatgccacc accgccgcgt
      241 cgcgctcacg ctcattatgc agcgcctgat acaaactgcc gccaatggta ctcaacagcg
      301 ccagacctgc cagcatctgg atccaactta cgggcagggc agccatcatc ccggtaatgg
      361 cactaccaaa cagacctgcg agcaaataga aaatgcctgc accggcagct gccagccaac
      421 gttgatcttt atccggatgc gcttccgggc tttggcaaat agccgcggtg attgccgcaa
      481 taccgacgga ataaacgccg aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa
      541 ttaatggcga aacaggagtt gaatatccgg ctgctttcat tgctgcgata cccggtgcgt
      601 tttgcgatgc catcgtcacc agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg
      661 aaaaatcagg ggtaatataa gtggggagaa cgggtttaaa gacaacatca gttgtgacaa
      721 cgtcaccttg cgcgataacg atcacgatcc caataatcat cgcggcaatt accgcatagc
      781 gcggcgcaac ggccttggtt gccagccata ccagcaacat acttccacac aacgtaaatt
      841 gaccgtccag actggcaaac gcctgtaaac caaagcgtaa taaaatcccg gcaagcattg
      901 ccgccgcaag cgagtgcgga ataatgcgca tcagacgagc aaagagtccc gttatgccgc
      961 agaggactat taacgcgttg gtgaaaataa aaacgccgat ggcttcgtta agtgttagtc
     1021 cctgcaatcc ggtgaccaac aaagccgcgc caggcgttga ccatgcggtg agaacaggta
     1081 cgcgatacca taatgtcaga gtcagcgtac tgacgcccat tgccagcccc agcgccgtca
     1141 tccagccaga gatttgtgca gtggtggctc cggcgacaat cgctgcttgc cagattattg
     1201 ccgctgaact ggcgtagcca attaataccg ccagaaaccc cgccagtagc gtgggtggag
     1261 gaacagaaaa cagacgcata gtcacctcgt gcgttatagc gtacaggcac cgtagcattt
     1321 gtccgttata acgcacaagt gataaacttc cgttttgccg gaggagtcgc atggaaaatc
     1381 tcgctcgctt tttatccacc acacttaaac aactacgcca gcagcgcggc tggagtcttt
     1441 cgcgactggc agaagcgacg ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg
     1501 agtccagtcc gacggtcgcg acattatgga aaattgccac cgggttgaac gtaccatttt
     1561 caacatttat ttctccgccg cagcccgcta cgccttcagt ttatgatcca caacagcacg
     1621 cgatggtgat cacttcgctg tttccttatg atccacaact ctgctttgaa cacttctcga
     1681 ttcagatggc acccggtgca ataagcgaat cgacgcccca tgagaagggg gtaatcgaac
     1741 atgtcgtcgt cattgatgga caacttgatc tgtgcgttga tggggaatgg cagaccctta
     1801 attgtgggga aggcgttcga tttgctgcag acgtcacgca tatctatcgt aatggtgggg
     1861 agcaaaccgt acattttcat tccctcatcc attacccgcg cagttaagcg ggaaaactat
     1921 ttcgcaacgt cgcgcttgtg gctaaaatag ccgccatttt tcagctactg gataagaatg
     1981 tgaccgtatc ttctcatcga cttgaactgt taagcccggc acgcgatgcc gccattgccc
     2041 gcgaagctat tttgcacggt gccgatgctg tttatatcgg cggccctggt tttggtgccc
     2101 gtcataatgc cagtaatagc ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt
     2161 atggtgcaaa aattttcgtc acgcttaaca ccattttgca tgatgatgag ctggaacccg
     2221 cgcaacggct gattactgac ctctaccaga ccggtgtcga tgcgctgatt gttcaggata
     2281 tggggattct ggaacttgat attccgccga ttgaactgca cgccagtacg cagtgcgaca
     2341 ttcgtacagt tgaaaaagcg aagttcctct ctgatgttgg cttcacgcag attgtgctgg
     2401 cgcgagagct gaatcttgat cagatccgcg cgattcacca ggctacggac gcgaccattg
     2461 aattctttat tcatggggca ctgtgcgtgg cctattcggg tcagtgctac atttctcatg
     2521 cgcaaacagg gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt ttgccataca
     2581 cattgaaaga cgatcagggg cgggtggttt cctatgaaaa acatctgctg tcgatgaaag
     2641 ataacgatca gacagccaac ctcggcgcgc tgattgatgc tggcgtacgc tccttcaaga
     2701 ttgaagggcg ttacaaagat atgagctacg tgaagaatat caccgcccat tatcgccaga
     2761 tgctggatgc cattattgaa gaacgtggcg atctgacgcg tgcttcatca ggtcgtactg
     2821 aacatttctt tgttccatcg acggaaaaga ctttccaccg tggtagcaca gattattttg
     2881 tgaatgcccg taaaggcgat attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg
     2941 tgggtgaagt attgaaagtg gcgaaagatc atctcgatgt tgccgtcacc gagccactgg
     3001 caaatggtga tggcctgaac gtgatgatta aacgtgaagt catcggtttt cgtgccaata
     3061 cggtcgagaa aaccagagaa aatcagtacc gcgtctggcc caatgaaatg ccagcagatt
     3121 tgcacaaaat tcgtccacat cacccactaa accgtaatct tgatcataac tggcagcagg
     3181 cactgacaaa aacctccagc gaacgtcggg tggcggtaga cattgaactg ggcggctggc
     3241 aggaacaact gattctgacc ctcaccagtg aagagggtgt cagcatcacg catacgctgg
     3301 acgggcagtt cgacgaagcc aataacgccg aaaaagcaat gaacaatctg aaggatggtc
     3361 tggcaaaact ggggcaaacc ctctattacg cccgcgatgt gcaaattaat ttgccggggg
     3421 cgctgtttgt accaaacagt ctgttaaacc agttccgccg tgaagctgct gacatgctgg
     3481 atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa accggttgct gatcctgcgc
     3541 cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg
     3601 aattttatca tcgctatggt gtgcagctga ttgacgcggc gtatgaagca catgaagaga
     3661 agggcgaagt cccggtgatg atcaccaagc attgtctgcg ctttgccttt aatctgtgcc
     3721 cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg
     3781 gcgatgaagt attaacgcta aagtttgatt gccgcccatg cgagatgcac gtcattggca
     3841 aaatcaaaaa tcacatactg aaaatgccgt taccgggaag cgtagtggca tccgtaagtc
     3901 cggatgagct gctgaaaaca ttgcctaagc gaaaagggta aaacgccagt tttctggtta
     3961 ctcacaactc attgaatctg catgatattg cctgccgggt aaggcgtctc gccgcatccg
     4021 gcaacaaatg actcaacgtt gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg
     4081 ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc aattcatcgc tgtccgcttc
     4141 atgacgcagc tgatgatcca tattctttac ccacacaaat tcatgtcctt tgtgccctgc
     4201 catgagttgt cctgagaaca gcgcacacgt taataagaca accgataacg ccttcgtaaa
     4261 catcctgcca cctttttctt accttttgcc gctatgatgc cgatcgtttc ttgaggttat
     4321 tattcagttt tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg attttgcatt
     4381 gcattctgcc gttgcggcga tttagtgcta ttttcgagca aattacacac agaggtaaac
     4441 actaatgttt actttgttga tatactcagc ggcagggagg cgatgtgaaa caaagcgagt
     4501 tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa tggccgcaac catttgaaac
     4561 tcaggtttca tgggaggcgc agtgtcatgc cgcgtcactc ctgcgtaaag caatcctgaa
     4621 acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag agacagtcga
     4681 aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata tggtttcttt
     4741 tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc gctgaagcga tggaagcggc
     4801 aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc ttatcccgtt
     4861 accttcgcca ttaaatagtc atgatcactt tattgaagta cctttgagcg tcgcctctaa
     4921 agtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt tagccaggcg
     4981 aattggcaaa cctaaacagg agattactcg cctatttaac ttgcaccatg cgacaaaaat
     5041 cgacgccgtc cagcttgcgg caaaggcgct tggcaaagag ttatcgctgg tgatggttta
     5101 attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg aatcatctgc
     5161 acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat tacgcgagca
     5221 gattgcgtcg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg accaggtggc
     5281 gctttctggc atgagcttta tgactgtcag ccatgcctat cagttgctcg aaagtctggg
     5341 atatattatc gcgcgaccgc agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc
     5401 gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca cttatatttt
     5461 tgatatgttg caggccagtc gcgatccgtc ggtcgttccg tttgcctcgg cctttcccga
     5521 cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc
     5581 cacagcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc gtcaggctat
     5641 tgctcgtcgc tatgccttac agggcatcac catttctcct gatgaaattg tcattactgc
     5701 cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg gcgattgggt
     5761 gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc tacggctgaa
     5821 ggcgttatcg gtggcgacgg atgttaaaga agggatcgat cttcaggcgc tggaactggc
     5881 gttgcaggat tatccggtga aagcgtgctg gctgatgact aatagccaga acccactcgg
     5941 atttacctta acgccgcaaa aaaaagcaca actggtggcg ttactcaatc agtacaacgt
     6001 aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa aaccgctgcc
     6061 tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct
     6121 ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcac
//
LOCUS       DAHBHC010000159         6133 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHBHC010000159 DAHBHC010000000
VERSION     DAHBHC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.163"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..183
                     /locus_tag="M2A35_004852"
     CDS             <1..183
                     /locus_tag="M2A35_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201032.1"
                     /translation="AAFCRDAGSAGHGATEAARPERSLQARGFHASDRSDSPKPRQRV
                     RRRLAGAPAAACRSRA"
     gene            complement(325..657)
                     /locus_tag="M2A35_004853"
     CDS             complement(325..657)
                     /locus_tag="M2A35_004853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201033.1"
                     /translation="MDSRQGGALWRMKYDGDGRATARLFSGKGCRRAPLFTAPADAAR
                     HKRCLWSVSRVRRARDGRFYRSRLPAPGKDRAPVALKGAALTRRPFHYADVLMGASVS
                     SCLSFVCY"
     gene            complement(621..1523)
                     /locus_tag="M2A35_004854"
     CDS             complement(621..1523)
                     /locus_tag="M2A35_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCB7201034.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QNTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDVQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLNVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDDLPEVTGPAWIVDKVAHYGG"
     gene            1962..2921
                     /locus_tag="M2A35_004855"
     CDS             1962..2921
                     /locus_tag="M2A35_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCB7201035.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            2921..3316
                     /locus_tag="M2A35_004856"
     CDS             2921..3316
                     /locus_tag="M2A35_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCB7201036.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSASINPKDDFEEQTRRNLSMLKK"
     gene            complement(3457..3795)
                     /locus_tag="M2A35_004857"
     CDS             complement(3457..3795)
                     /locus_tag="M2A35_004857"
                     /inference="COORDINATES: protein motif:HMM:NF025056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201037.1"
                     /translation="MSVFMERSIREADRVLVICTDRYCYKARGRVGGVGYESHLLTSE
                     IMQEMGTIKFIPIIRNLTNGVSLPDALSGRFYYNLSDGAEYHNNFELLLRELHDAPVP
                     IPPIGINPFC"
     gene            complement(3820..4368)
                     /locus_tag="M2A35_004858"
     CDS             complement(3820..4368)
                     /locus_tag="M2A35_004858"
                     /inference="COORDINATES: protein motif:HMM:NF019956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201038.1"
                     /translation="MFNLLMSGMENTWDAPTWVLPNDRYLEYTHPDIKAEFGSLNDQV
                     VTRLKSFPALFCYERYIDSPAKVGQITEIERRTRELKITYSINHDIPFITQKQLFDIF
                     PYLGVEPDTFELTRTHWALKDVNLINVLRTKKIIQRQLLQSQRRPPKVFITYSWDSPE
                     HKQWVAQLAGCLRNNGIDAFLD"
     gene            complement(4471..5643)
                     /locus_tag="M2A35_004859"
     CDS             complement(4471..5643)
                     /locus_tag="M2A35_004859"
                     /inference="COORDINATES: protein motif:HMM:NF017519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201039.1"
                     /translation="MLIKNKSKIHLSPHFMLNYELLQPGDIILERGCAWYSEKIAKHT
                     NSRYSHAMIYVGGTIIEATRGGGGYSRVPNRSTVRDISDFKVLRLKEFPGKDILQVIC
                     DHARSLCGSQYSVSQALKVKGPDFLRKFANDSRKQFCSRLVAQCYQKAGIQLTENINF
                     CSPGDIERSDYLVEQPEMVYRASEEDVAHAQGESPHTQHTKNAVNFVRNALSIFENYG
                     IKTVGSSDGEVVITTLNDITLAVYENRNKPGLDEEITEAMRLSGYLDHIDLDRQKNPY
                     RYDLMLFSQKVEVHGNENKTLEILRHELKKEQDIVEVRLRSYLAGRHNLNSGLKFNEL
                     EFTIAHGLLKGMLERVIVIEKYTVTKRNDVRFKMINVACNRVIQNITMKAPELNIL"
     gene            complement(5907..>6133)
                     /locus_tag="M2A35_004860"
     CDS             complement(5907..>6133)
                     /locus_tag="M2A35_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7201040.1"
                     /translation="YIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVI
                     HPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
BASE COUNT         1829 a         1486 c         1242 g         1576 t
ORIGIN      
        1 gcggcctttt gccgggatgc cgggagcgca ggccacggcg cgacagaggc ggcgcgaccg
       61 gaacggtcgc tgcaagcccg gggttttcac gcatcagacc gtagcgacag cccgaaaccg
      121 cgccagcggg ttcggcggag actggcaggt gcgccagcag ctgcctgtcg gagtcgggcc
      181 tgacgaccgg atgccgccag gcagccggga gccgaaggtg cgcccggcag acgggacaac
      241 actgcagtag tcagaatcag agcagcagga aaaagaagaa cagtagcagt agcagtagca
      301 gtagcagtag caggaacaac aacatcagta acagacaaat gacaggcatg aagaaacaga
      361 agcccccatc agaacgtccg cgtaatgaaa ggggcggcgc gtcagcgcgg cccctttcag
      421 cgccaccggc gcgcggtctt ttcccggcgc gggcagccgg gagcggtaaa agcgcccgtc
      481 ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg ccgcgtcagc
      541 cggcgcagta aaaagaggag cacggcgaca tcctttaccg gaaaagagac gggcggttgc
      601 ccgcccgtct ccgtcatact tcatccgcca taatgcgcca ccttgtcgac tatccacgca
      661 ggtccggtga cttccggcag gtcatcgtca tctgtcgggg aagaccattc cagttccccc
      721 cacagcacat caacgagctc tccgcgctca tacaactgcc agccacagaa attgcagccc
      781 tgttcggcgt accagtgctc cagtgtgcag ccgaaacggc ggcttaattc tgcaacaacg
      841 ttgctttccg gctgacacca cggcgtgtcg aaatccacct gaatgaagtt ttctccctga
      901 cggctgatat tcacctcata ccccacaggc cacttcacgc catactgttc cgtataccag
      961 tgatacgcag aaggaacacc gttcagaaca ccaccgttaa agccattcac ttcaacattc
     1021 aggcgggtgg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg cagtacattg
     1081 tcacacagac ggttccacca caccgataca tcctcgttac tccagaagcc acaccagtca
     1141 cctcttttgg cggtgaaatg cacggtgatg gtttccctga cctcatccgg taatccttcc
     1201 cagcgacgct ggccagtacc actctgcagc cagagttcat gcagcatcag gcagttttgt
     1261 acatccagta gcacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc gatattctcc
     1321 ggcgatacaa caccacgtcc ggcagccgtc agtccggggc acggttcaaa ctgcacatct
     1381 tcagtgttct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc ttcatttgtg
     1441 gcgcggcgat aaaacggtgt gactgcaccg ctggccagtc gtttaatctc agcaatctgt
     1501 gccggttcac cagaaaaatg catacgattt gagcaccagt ttggcatggg aattctcctc
     1561 tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc
     1621 aggtgcggcc ccgaagccgc agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg
     1681 aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc
     1741 tggttaagta gtttccgtct cagtttgtga cgcaaaatga gaaaccaaca acaactcaaa
     1801 ttgagtcaca ataagaacac aaagagactc taaaagagcc acattacttg acattaaccc
     1861 aaaatgagac acaataagaa ataacaaaaa ctcaaaacga tgcatcgaca taaaagatgc
     1921 tcaatttgag acaaacacaa actcaaaacg aggcaaaaag aatgctaaaa gtatcatgcg
     1981 atgatggttc aactaacgta aaactggcct ggcttgaaga tggcgaagta agaaccagtc
     2041 tgtcaggaaa cagctttaaa gaaggctgga acccgggact gtttaatgcc ggcaaagttt
     2101 acaattacgt cgtggatgga aaaaaataca cctatgattt gggcagcact gctgtcatag
     2161 gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata catcatgcac
     2221 tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta cctgtaaccg
     2281 aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa aaggcaaatg
     2341 ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa gttaatgtaa
     2401 tgcctgaatc tttgccggct gcatttgaat cactaaaaaa agataaagta aacaaacttg
     2461 aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta attctcggtg
     2521 catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc cgcataactc
     2581 ataccgtcat gaatgcacta acaaaagcct ccacaccctg caattatttt attgctgatg
     2641 agttaataaa aaacaggcat gataatgaat atctccagac attaataaat gatgtggctg
     2701 aaataaaaaa tattactcat gtaatagaca gtgaggtgaa atctctggca gaaagcatca
     2761 ggcaggaaat ttcaacattc tcaggaatga accgaatcta cctcacaggc gggggagccg
     2821 agcttattta ccctcacata aagcaatact tcccgaatct gaaagtgaac aaggtagatg
     2881 aacctcagtt tgcattggta aaagcaatgg tgcatgcgta atggaaagca gtgatccaaa
     2941 aaaaagaaag aaagttgtgg catatctgca cccggcactt tatccgcagg acaacctgac
     3001 acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca gacaatccct
     3061 tatttgcgga gcagctcttt attctgttga cccccgactg ttaacactaa tcagcggttt
     3121 tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa caacaggata
     3181 cacatcgaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat cttcagaaaa
     3241 caaatcagcg agcatcaatc cgaaagatga tttcgaagaa caaaccagac gcaatctgtc
     3301 tatgttaaaa aaatgacaaa gaggggcaga atatattctg cccactctaa ttttcacaat
     3361 acttatgttc tgtaaagcat ccaactgtag ttgaaaaact caaatgtctt tgagtttaat
     3421 atttttacat ctatcattcc taaataagaa tgaacactaa caaaaggggt ttataccaat
     3481 aggaggtata ggaacaggag catcatgtag ctctcgaaga agcagctcaa aattattatg
     3541 atattcggct ccatcactca aattgtagta aaatcgtcct gacaatgcat ctggcaaaga
     3601 gaccccatta gttagatttc ttataattgg aataaatttt atagttccca tttcttgcat
     3661 tatctcgcta gtcaaaagat ggctctcata tccgacacct ccaactcgac cacgagcttt
     3721 ataacaatat cgatccgtac aaatgaccaa aactctatca gcttccctaa ttgatctctc
     3781 cataaatact gacatatcct cccctcccct aatattccat tagtcaagaa atgcatcaat
     3841 tccattattt ctcagacagc cagctaattg agcgacccat tgtttatgtt cgggagaatc
     3901 ccaagagtat gtaataaata cttttggcgg tctcctctgc gactggagca actgtctttg
     3961 aattatcttt ttagttctta acacattaat taagttaaca tcttttaacg cccagtgggt
     4021 tcgggttagt tcaaaagtat caggttctac tcctaaataa gggaaaatat cgaataactg
     4081 cttttgggta atgaagggaa tatcatggtt tattgaataa gttattttta attctctagt
     4141 acgtctttca atttccgtaa tctgaccaac tttcgccgga gaatcaatgt aacgctcata
     4201 acaaaaaagg gcaggaaatg attttagtct agttacaact tggtcattaa gagaaccaaa
     4261 ttcagccttg atgtcaggat gagtatactc cagataacga tcatttggta aaacccacgt
     4321 tggagcatcc catgtgtttt ccataccaga cattaacaag ttaaacataa tctaccttaa
     4381 taacagtaaa ggacatcatt cagcgaagtc attaactgac agcacacatt agcccagttg
     4441 gagaataatg cacggttcag ttcaacgctc tcacaatata ttcaattcgg gagctttcat
     4501 cgtgatattc tgaatgaccc tgttgcatgc aacattaatc attttgaatc taacgtcgtt
     4561 tcttttagtt actgtatatt tttcaataac aataactcgc tccagcattc ccttcagtaa
     4621 accatgcgcg attgtaaatt ctaattcatt aaacttcaga ccactgttca aattatgtct
     4681 tcctgccaag taagaccgaa gacgaacttc aactatgtcc tgttctttct tgagttcatg
     4741 tctaagaatt tccaaagtct tattttcatt accatgaacc tcgacttttt gactaaagag
     4801 catgagatca tacctgtaag ggtttttctg cctatccaga tcgatatgat caaggtaacc
     4861 agataatcgc atggcttctg taatttcttc atctaatcca ggtttatttc tattttcata
     4921 tactgctaaa gttatatcat ttaacgtcgt aataacgact tctccatctg aagagccgac
     4981 agtcttaatt ccataatttt cgaaaatact caatgcattt ctaacaaaat ttactgcatt
     5041 ttttgtatgt tgtgtatgcg gcgactcccc ctgcgcgtgt gcaacatctt cttccgatgc
     5101 acgatagacc atctcaggct gttcgactaa gtaatcgctt ctctcaatat cccctggtga
     5161 acagaaattt atattctctg tcagttgtat tccagccttc tggtaacatt gagccacaag
     5221 ccgtgaacaa aattgttttc tactgtcgtt ggcaaatttt cttaaaaaat ccggcccttt
     5281 aaccttgaga gcctggctta ctgaatactg gctaccacag agactacgag catggtcaca
     5341 tattacttgt aagatatcct ttcccggaaa ctctttaagc cttagtacct taaagtcgct
     5401 aatatcacgt acagtagaac ggtttggaac tcggctatac cccccacctc ccctagttgc
     5461 ctcaattatt gtcccaccta cgtatatcat tgcatgagag taacgactat tggtgtgttt
     5521 agctattttt tctgaatacc aagcacaacc tctctccaaa ataatatctc ctggttgtag
     5581 gagttcataa ttaagcataa aatgcgggct caaatgaatt tttgatttat tttttattaa
     5641 catattaatt atctttagta cctgtactat tagaactctt atctttagtt aaaacactta
     5701 tcagatagtg tttcctttta tactcgatta ttactgcaag attgtgcaat gctgtttttg
     5761 cgttcatttt ggcataaacg agatatagat tttccaatca tttcacaaac ctttatgcta
     5821 ttttaactac ccaactgttt ttctatgtat gccacaacag taacaacaga taatcagccc
     5881 ccagtaaggt aaaaatatac tcttctttat ggccacttct gtgctgtatg caacactcgc
     5941 agaatataca cttttcccca ctggctatcg acctcataaa ccagaacaaa atgcgggtga
     6001 ataaccagtt cccgggttcc ttccacacga ccgttgcggc ctttatacgg ctgcccggcc
     6061 aggctgctgg ctgaataacc aatccgttcg tccagttcaa tggcggccat caggttctgc
     6121 tccgcaatgt act
//
LOCUS       DAHBHC010000160         6090 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHBHC010000160 DAHBHC010000000
VERSION     DAHBHC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.164"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..171)
                     /locus_tag="M2A35_004861"
     CDS             complement(<1..171)
                     /locus_tag="M2A35_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HCB7201041.1"
                     /translation="MTTPCLYSIIRYAPYAETEEFANVGVVLCAPKLGQFCFKLTQSN
                     NARVKSFFQDEII"
     gene            complement(168..905)
                     /locus_tag="M2A35_004862"
     CDS             complement(168..905)
                     /locus_tag="M2A35_004862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201042.1"
                     /translation="MQNNYLQVIAYTRRMNDGITKPFLCTCQDGLAYIVKGRPKLRQK
                     ELVAEFIAAHLAKQIGLPCPDFCIVDVDCGLIEFMPDLRGELLPGPAFATRFIENASI
                     INIQQARSAVTIQEQKKIFFFDRWINNADRSLTDIGGNVNIIYDAFNNRYYLIDHNLA
                     FAQDTRSHEYDVHVYSPNGRPWVFDMLDEPELMDLANDAISSVEEKFNQLPDEWFSTD
                     EERRLMLNEIMNCLNRVHDREFWSNIK"
     gene            complement(957..1673)
                     /locus_tag="M2A35_004863"
     CDS             complement(957..1673)
                     /locus_tag="M2A35_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCB7201043.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGVNALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPAGSKPS
                     FPDGMLILVDPEQPVDPGDFCIARLGGDEFTFKKLIKDSGQVFLQPLNPQFPIIPCNE
                     NCNIVGKVIASQWPEETFGS"
     gene            1774..1974
                     /locus_tag="M2A35_004864"
     CDS             1774..1974
                     /locus_tag="M2A35_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCB7201044.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEIKPFPSNKKTTA"
     gene            2081..2377
                     /locus_tag="M2A35_004865"
     CDS             2081..2377
                     /locus_tag="M2A35_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201045.1"
                     /translation="MTQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            2410..2556
                     /locus_tag="M2A35_004866"
     CDS             2410..2556
                     /locus_tag="M2A35_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCB7201046.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLTQKE
                     KGHE"
     gene            2549..3409
                     /locus_tag="M2A35_004867"
     CDS             2549..3409
                     /locus_tag="M2A35_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201047.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSSDRHQETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQTQSKPSSAITV
                     SKTGYVFFDR"
     gene            3517..5397
                     /locus_tag="M2A35_004868"
     CDS             3517..5397
                     /locus_tag="M2A35_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCB7201048.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LRVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKGFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAKEIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            5480..>6090
                     /locus_tag="M2A35_004869"
     CDS             5480..>6090
                     /locus_tag="M2A35_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCB7201049.1"
                     /translation="MRIKKLIDHDELLSTLSYDSETGIFKWLKTNSVVRVKGSIAGGV
                     SGGYICISINNVLYKAHRLAWFYVYKKWPPKFIDHVNGNRLDNRISNLRLATEEQNAR
                     NIVGNRLNTSGAIGVSWYKPTGRWKSYVGYKNKTISLGYFDSKEDAAFIAALARKKLY
                     GTYASKALNCEHELLSQFNNDEDKLAEYLKEKSKRTRKRVKNR"
BASE COUNT         1851 a         1289 c         1424 g         1526 t
ORIGIN      
        1 aatgatttca tcctgaaaaa agctttttac tctggcattg ttgctttggg tcagtttaaa
       61 gcaaaactga cctaatttcg gggcacaaag aaccacaccg acattagcaa actcttcggt
      121 ttctgcatat ggagcatatc taataatgct atacaggcat ggtgtggtca tttgatgtta
      181 ctccaaaact ctctgtcgtg cactctgttc aggcagttca ttatctcatt cagcattagc
      241 cttcgttctt catcggttga aaaccactca tccggaagct gattaaattt ctcctctact
      301 gaactaatag catcatttgc aagatccatc agttcgggtt cgtcgagcat gtcgaaaacc
      361 cacggtcttc cattcggcga atacacatgg acatcatact catgagatct tgtatcctgc
      421 gcgaacgcca gattatggtc aatcaggtaa tacctattgt taaaggcatc gtaaattatg
      481 tttacattac caccaatgtc agtcaatgat ctgtctgcgt tgtttatcca gcggtcaaag
      541 aagaatattt tcttttgttc ctgaatggtt acagcactcc tggcttgttg aatatttatg
      601 attgatgcat tttcaataaa gcgggtagca aatgcaggtc caggcaaaag ctcaccacgc
      661 aagtcaggca tgaactcgat taacccacaa tcaacatcaa caatgcaaaa atcaggacat
      721 ggcagaccta tctgttttgc tagatgggcg gcaataaatt ctgctacaag ctctttttgg
      781 cgaagctttg gccttccttt aactatgtaa gccagcccat cttggcaagt acaaaggaat
      841 ggcttggtaa taccatcatt catgcgccta gtataggcta taacctgtag atagttattt
      901 tgcaaaacct acttttccta gatataggca ccaaacatag atgtttgaaa actaattcac
      961 gacccaaacg tctcttcagg ccattgactg gcgataacct tgcctacaat gttgcagttc
     1021 tcattgcatg ggatgattgg aaactgcggg ttaagtggtt gcaaaaacac ttgcccacta
     1081 tctttgatca gttttttgaa tgtgaattca tcaccaccaa gtctagcgat acagaaatca
     1141 ccaggatcaa caggttgctc agggtcaacc aagattaaca ttccatcagg aaagcttggt
     1201 ttcgatcctg ccggagctgt cattgagttg ccttcaacct caagccagaa tgcagaatca
     1261 ctggcttttt tggttgtgct tacccatctc tccgcatcac ctttggtaaa ggttctaagc
     1321 tcaggcgaga acatcccggc ctgaacatga gaaaaaacag ggtactcata ctcacttctg
     1381 agtgatggct gcatactaac cgcttcatac atctcgtaga tttctctggc gattgaaggg
     1441 ctaaattctt caacgctaac gttgagaatt tttgcaagca atgcagcgtt ataagcattt
     1501 aatgcattga cgccattaaa taaagctcca acacctgact gccccatccc catcttgtct
     1561 gcgacagatt cctgagataa gccaagttca tttttctttt tttcataaat agctttaaga
     1621 cgacgtgcgt cctcaagctg ctcttgtgtt aacggtttct tttttgcgct catacgttaa
     1681 atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt tacctctagc
     1741 ggtgataata attgcatgta ctaaggaggt tgtatggaac aacgcataac cctgaaagat
     1801 tatgcaatgc gctttggtca aaccaagaca gctaaagatc tcggcgtata tcaaagcgcg
     1861 atcaacaagg ccattcatgc tggccgaaag atttttttaa ccataaacgc cgatggaagc
     1921 gtttatgcgg aagagataaa acccttccca agtaacaaaa aaacaactgc ataagtaaca
     1981 ccgctcttta tcaatctgca ccgccgacaa cgcggtaact aattaagcac tcatctaaaa
     2041 atgagtatta gtgattattt acctatggaa atagtaagaa atgacacaaa caagttacag
     2101 caaactatca cagcgcgacg ttgatcgcgc agaaacagat ttacttatca acctgtcaac
     2161 gcttacccag cgtggtctgg caaagatgat tggctgtcat gaatcgaaga taagcagaac
     2221 ggactggagg tttattgctt cggtcttgtg tgcttttggc atggcatcag acatcagtcc
     2281 gattagcaga gcttttaagt atgcgcttga tgaaatcaca aagaaaaaat ccccggccgc
     2341 caccgaggat tttaagcaaa ttgatatgca attctgaggg aattactgga tcaatccaca
     2401 ggagtaatta tgacaaaaca actcagtcct taccaggaca aaattcacaa acacatacta
     2461 cgtgatcgct tcctgtccag cttcaagcag cctggtcgat tccgggctga gttggaaaaa
     2521 gtgaagctaa cgcagaagga gaaaggtcat gagtaatctt gcaaccgtaa cacatttaag
     2581 gccttcacaa cggcctgtgg agcgtcgtgt ggcagaagtt gaagatggtt atacccgtct
     2641 tgcaaatgcc ctgtatgaag agcttatcgg cgcagattta acgaaaaatc agagcaaggt
     2701 tgcccacgcc atatgccgta aaacatacgg ctacggtaaa aagatggatc gcatttctga
     2761 tagtcagtta gctcaaatta ccaggctgcc aagacagaag gtaaacaagg ccaagaatga
     2821 gcttatcgcg atgaaggtta tccttcgcga aggccagcaa atcgggccta acaagaacat
     2881 cgaagaatgg caaatcgaag ggtgtcacta ctctggtgat aatgtcactg cattggtgac
     2941 aaaaagtgtc accaaaacgg tgacagcgct gtcaccaaaa cagggacaca caaaagaaac
     3001 tattacaaaa gaaaaaagaa ataataaaaa cactatgtcc gaaagtgttc ggacggagtg
     3061 tgaaaaatca tctgaccgtc accaagaaac cgacaaggca ttcgaggaaa tattctggtg
     3121 tgcaggcatg cggaaagccg ggaagaaaaa cgcggcttcg gcattcagaa cacagttcag
     3181 ggaatggcgt aaaactacca ggggtacggc aagcgagttt gccacgatgc tggcagaaga
     3241 catcgcatgc aggaatggta agcagttcgg attcgacagg ttgttaccat cgagctacct
     3301 gaacggtcaa cgctggaacg acgagaagcc agaaactatt caaacacaat ccaaaccatc
     3361 atccgcaatc accgtatcga aaactggcta cgtgtttttc gacaggtgaa ccatgaaatc
     3421 aaaaatcaaa tcgctactgg tcgctggtta taaccacggc tggttaagta tttcgtttgt
     3481 cgatttctgg tttaaaaatc tcaatctgag ggaatcatga ggccaagtga acttagcgac
     3541 ctgctttggg cgcaggttga cagggtggct ccgcacctgt tgccaaacgg caagaaagag
     3601 gggcatgagt gggttgccgg taacgttaac ggtgacaagg gaaacagcct tagggtcaac
     3661 cttagcggca agaaaaaatg ggctgatttc gctgagggag acggcggtga catgcttgat
     3721 ttgtggatgg catgtcgtgg aattaacctg catcaggcta tgcaggaagc gaaaggattt
     3781 ctcggtatca aggatgacga tcatcatttc gatgccaaac gtgagaagaa attctccaga
     3841 cctgatcgca agaaaatcgc ccgctacgtt accagaacag aatcccatct tgagtacctg
     3901 caatcgcgtg gcatatcgcc agaagtcgta aagcgctacg aggttgtcag cggcaaggtg
     3961 tggaatggag aacgagaact tgatgcactg gtgcttccgt acaaacgcga tggtgagttg
     4021 ttgcaggtca agcgaatcag cactgagcgc ccggacggga agaaagtcat tatggcagaa
     4081 ggtgattgcg aaccttgtct gttcggatgg caggctctgg acgctggcgt gagggcggtt
     4141 gtactttgcg aaggcgaaat tgattgtatg agctatgcac aatacggcat ctcggcgtta
     4201 tccgtgccgt ttggtggcgg gaaaggcgct aagcaacagt ggattgagtt tgagtatcac
     4261 aatctcgaca ggtttgagga aatattcatc tcgatggacg ttgatgatgt tggtcgtgaa
     4321 gccgcaaagg aaatcgcaag ccgactcggt gaacatcgtt gccgtcttgt tactctgccg
     4381 tacaaagaca tcaacgaatg cctgatgaac ggtgttaccg aggatgaaat ctggcagtac
     4441 atcggcacgg catcctactt cgatcctgaa gaactctaca gcgcgcgaga gttttaccag
     4501 gacactatca acgctttcta cggcaagcag cagtatctgt ttaatccacc gtgggaatct
     4561 ctggcagata aattccagtt ccgtgaggca gagttgacgc tggtcaatgg tgtgaacggt
     4621 cacggaaaaa cggaggttgt cgggcatatg gcacttgagg caatgcgtca gggtgtgaag
     4681 acatgcatcg cgtcacttga gctgaagcct ggtattctcc ttaagcgcct tacccgtcag
     4741 gcgacgtgct gcaagatgcc gccagtgctg gaaattgact ctgcatttaa attttatgac
     4801 gaaagacttt gggtgtttgg cctgaccgga acggcgaaag ccgacaggct gatcgaaata
     4861 ttcgactacg ctcgccgccg atacgggatc cagttattca tcatcgacag cctgatgaaa
     4921 tgtggcatag gcgacgatga ctataacggg cagaaggcgt ttgtcgactc gatttgcgac
     4981 ttcaaaaaca aaacaaactc ccacgtcatt ctcgttactc actcgcgaaa aggagacagc
     5041 gaagaaaaac caaccgggaa aatggacgta aaaggctctg gagcgataac agacctgaca
     5101 gacaaccttt tcatcatctg gcgtaacaag gctcgcgaga gagcgttaca gagagttcag
     5161 agtggtgaaa agatgtcaga gaaggacgaa cagctactgg catctccggc atctgttttg
     5221 atgcttgaaa aacaacgtaa cggcgaaggt tgggaaggtg gtgtcccgtt gttccttgac
     5281 gagcaatcgc accagttcct gcaacttgaa tcaggatcgc catatagcta catcgccaat
     5341 atgccgaaat cggaatatga cgaggcgtgg cgacaggaaa acgtgacgga gtattaaatg
     5401 gccatctaca tcactgagct aataacaggg ttatcgttac taatggttct tactgtatat
     5461 attattaagt atattcttta tgcgaataaa aaaactaatt gatcacgacg agcttctgtc
     5521 aacattatca tatgactcag aaacaggaat atttaaatgg ctaaaaacaa attcagtagt
     5581 aagagtaaaa ggtagtattg ctggaggtgt tagtggtggt tatatatgca ttagtataaa
     5641 taatgttttg tataaggcgc atagacttgc ttggttctat gtatacaaaa aatggcctcc
     5701 taagtttatt gatcatgtaa atgggaacag acttgacaat aggatttcaa acctaagact
     5761 ggcaacagaa gagcagaatg caagaaacat tgtagggaat agattaaaca catccggtgc
     5821 aattggagtg tcttggtata agccaactgg caggtggaag tcttatgttg gttataaaaa
     5881 taagacaata tcgttagggt atttcgatag caaagaagat gcagcattca tagcagcact
     5941 agcaagaaag aaactatatg gaacttatgc gagtaaagca cttaattgcg agcatgagct
     6001 tttatctcaa tttaataatg atgaggataa acttgcggaa tatcttaagg aaaaatctaa
     6061 aaggactcga aagcgtgtta aaaacagata
//
LOCUS       DAHBHC010000161         6066 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHBHC010000161 DAHBHC010000000
VERSION     DAHBHC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.165"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..213
                     /locus_tag="M2A35_004870"
     CDS             <1..213
                     /locus_tag="M2A35_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCB7201050.1"
                     /translation="KQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPT
                     SGVIMVTHQPGVWNLADDICDISAVL"
     gene            251..2623
                     /locus_tag="M2A35_004871"
     CDS             251..2623
                     /locus_tag="M2A35_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCB7201051.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAEGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKCNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            2680..5463
                     /locus_tag="M2A35_004872"
     CDS             2680..5463
                     /locus_tag="M2A35_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCB7201052.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMFETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRTT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     YPVNTYTGVLLPK"
     gene            5825..>6066
                     /locus_tag="M2A35_004873"
     CDS             5825..>6066
                     /locus_tag="M2A35_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCB7201053.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         1784 a         1360 c         1374 g         1548 t
ORIGIN      
        1 aaacaacgta tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac
       61 gaaactacct ctcatcttga ggaacaagag gctatccgct tactgcgttt agtgcgtgaa
      121 aaactaccca caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggca
      181 gatgatattt gtgacattag cgcggtttta taaaaacaat aaaagccctg ctgtacggag
      241 atataaataa atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc
      301 gcaggccgtc gatgacaaaa acatgtacat gcatgttttt gaagagatga cggtctatgc
      361 tcctgtccct gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt
      421 accgaccggg aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga
      481 ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc ccggagaaaa tctctattca
      541 cggtgcgtcg ccctatcaga atgcctattt gattgacggt attagtgcca ctaataactt
      601 gaacccagcg aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg
      661 gtattatctt gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt
      721 tgaattcggt cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga
      781 tgatagcagc gtgaaactgg gttatcgcac tacgcgttcg gactggttaa catcgcatat
      841 cgatgagaat aacaagagcg catttaatca aggctcttca ggaagtactt attactcccc
      901 agattttaaa aagaactttt ataccttgtc gtttaatcag gaactcgctg ataactttgg
      961 cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa
     1021 tgacggcatt gtcgccggtc gggcacagta taaaaacgtt atcgataccg cattgagcaa
     1081 atttacctgg tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc
     1141 cagccgtgat tataatacca gcaccttccc gcagtctgat cgcgaaatgg gtaataaatc
     1201 ctatggtctg gcatgggata tggatacgca gctcgcatgg gccaaactac gtaccaccgt
     1261 tggttgggat catattagtg attatacccg tcacgatcat gacatctggt acaccgaact
     1321 ttcatgtaca tatggtgata ttacagggcg ttgcacccgt ggcggattag gacacatttc
     1381 ccaggctgta gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgaggg
     1441 taatgtttcg catcaaccct acttcggcgc ggaatacatc tattccgatg cgtggactga
     1501 acgccataac cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa ctaaccatac
     1561 catttaccat aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga
     1621 tcgcattagc tggcgtaatg tgtcattaat gcccggcgtg cggtatgact atgacaacta
     1681 tctgtcaaac cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctaatca
     1741 aacctcaatg attacagcag gttataaccg ttactatggc gggaatattc ttgatatggg
     1801 attacgtgat atccgcaata gctggacgga atcggtatca ggtaataaaa ctctgacgcg
     1861 ttatcaggat ttgaaaacgc cttataacga tgaactggca atgggattgc agcagaaaat
     1921 cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg atcaaataag
     1981 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     2041 caaaaccaaa acgcattcgt tcagcctcag ttttgaactg gccgaacccc tgcatatccg
     2101 ccaggtagat attaacccac aaattgtctt tagctatatc aagagcaagt gcaacttgtc
     2161 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     2221 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     2281 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     2341 agaagcgcgt aaagctcgca ttatcctggg taagacaaat gcgcaataca tcagcgaata
     2401 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     2461 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     2521 caatgtattg gatagcaaaa ccgctgttga tacaacgaat accggtgtgg cgacctacgc
     2581 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     2641 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     2701 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     2761 gatgaaaagt taattaccgg gcaactgggc aatggcttgc gatatatgat ttatccgcat
     2821 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     2881 gaagacaatg agcgcggcgt agctcatttt gtagaacata tgatgtttaa cggcacaaaa
     2941 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     3001 gatgttaatg cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     3061 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     3121 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     3181 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
     3241 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa
     3301 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     3361 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     3421 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     3481 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
     3541 gtacaagtga acgatgagca aagctttatc gaacaagctg aatggagcat gttagttcag
     3601 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     3661 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     3721 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     3781 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     3841 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     3901 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     3961 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     4021 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
     4081 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
     4141 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
     4201 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccagg
     4261 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattactgc cgtatcgaat
     4321 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     4381 gtaagcggaa gcggcgttgg tgagctctcc tcttccagcc tgaaacgctg gagtgcggaa
     4441 aattcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     4501 cggactaata accctgaacc tggttttcag ttgattaacc agcgaatcac ccacagcacg
     4561 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     4621 gaccagcgtc cagcggagaa attcgcccag cagatgtttg agacgcgcta tgctgatgac
     4681 cgcacgaaat tactgcaaga aaatcagatt gtacagttta ctgccgcaga tgcgctggct
     4741 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     4801 tcagaagaca aactcgtggc gttaattacg cgttacttag gatcaatcaa acactctgat
     4861 tcgccattag ccgcaggtaa accattaact cgcacgacgg acaacgcatc ggttactgta
     4921 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     4981 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     5041 ctacgtgtta atattcgtga acaggcatct ggagcataca gcgtttcttc tcgcctctcg
     5101 gttgatcctc aggccaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     5161 catgatgaac tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc taaagggatc
     5221 agtgagcaag aactgaatga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     5281 cgtagcgttc aacaattagc gaacactatt gtaaatagtc ttattcaata tgacgatcct
     5341 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     5401 gccgttaaac aatatctttc ttatccggta aatacttata ccggagtatt attgccaaaa
     5461 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
     5521 gtaataattt tataaatgcg ttcaaaataa taatcaagta ctaatagtga tattttaagg
     5581 tctgattttt acgtgataat tcaggagaca cagaatgcgc ataaaaataa cagcataaaa
     5641 caccttacca ccacccaaga atttcatatt gcattgtttt tcaatgaaaa aatattattc
     5701 gcgtaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
     5761 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
     5821 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
     5881 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
     5941 cgatgtcgca ttccagatta tcaatgacga attatatctt gatggcaacg ctcgtcagaa
     6001 cctggccact ttctgccaga cctgggacga cgaaaatgtc cacaaattga tggatttatc
     6061 cattaa
//
LOCUS       DAHBHC010000162         6030 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHBHC010000162 DAHBHC010000000
VERSION     DAHBHC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.166"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(214..369)
                     /locus_tag="M2A35_004874"
     CDS             complement(214..369)
                     /locus_tag="M2A35_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCB7201054.1"
                     /translation="MDTIGLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(571..978)
                     /locus_tag="M2A35_004875"
     CDS             complement(571..978)
                     /locus_tag="M2A35_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201055.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPNNQPAILTEDQKELLQLFDALPDS
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1055..1282
                     /locus_tag="M2A35_004876"
     CDS             1055..1282
                     /locus_tag="M2A35_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7201056.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1266..1817
                     /locus_tag="M2A35_004877"
     CDS             1266..1817
                     /locus_tag="M2A35_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201057.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFDDGAVDQRALGVNRVKIFERWKSIDTRDKREKFTALIPAIMAAIRISDFRLY
                     REITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCATMG"
     gene            1789..2829
                     /locus_tag="M2A35_004878"
     CDS             1789..2829
                     /locus_tag="M2A35_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCB7201058.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVTEDEYTQKRE
                     SLKVGKIGTVNKNRTENGYVSAQNSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAVPPVFLDGLSEPIGKFPMTDNWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGAHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            2741..3283
                     /locus_tag="M2A35_004879"
     CDS             2741..3283
                     /locus_tag="M2A35_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201059.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQCMDCC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYPWSQPV
                     LYHICLEMRSKGIERQMTEGELKRLAERQLAKWAKHVGDGFSVPPVRRQLAAPKRPSG
                     PTPIELLKREYERRKAAGFV"
     gene            3313..3708
                     /locus_tag="M2A35_004880"
     CDS             3313..3708
                     /locus_tag="M2A35_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCB7201060.1"
                     /translation="MAKVFTSEQREELKARIIGLVRKNERMTMSQLERATGAGWHSVR
                     RCLVDVLACGDLYMSGEYGVFASEQAYREWRKTPEKTTDQTLIRKLPDGEIRRYDRHL
                     NIICRECRKSEVMQRVLAFYQGNFQEVMA"
     gene            3705..4004
                     /locus_tag="M2A35_004881"
     CDS             3705..4004
                     /locus_tag="M2A35_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCB7201061.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEANSE"
     gene            4051..4461
                     /locus_tag="M2A35_004882"
     CDS             4051..4461
                     /locus_tag="M2A35_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001541657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201062.1"
                     /translation="MWIEHPTLGNVPTYGGPLDSYTIPTKDSDGEFSCERYDHDFGGW
                     VESECLGLYLIDDKEQCRVYELEERVKELEAREVHLPTRYGLRYGHPINDDERHVMIP
                     KENGCWLYLADLEHALRVAGIHIKGEEYGNKTRR"
     gene            4439..4795
                     /locus_tag="M2A35_004883"
     CDS             4439..4795
                     /locus_tag="M2A35_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201063.1"
                     /translation="MGIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVQ
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4888..5106
                     /locus_tag="M2A35_004884"
     CDS             4888..5106
                     /locus_tag="M2A35_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCB7201064.1"
                     /translation="MTALFRKEYPRKSRATEFLFLILFIVLMIPISPLIFVWVIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            5108..5521
                     /locus_tag="M2A35_004885"
     CDS             5108..5521
                     /locus_tag="M2A35_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201065.1"
                     /translation="MITIFRENAEIKSFITGFLSDAAHDNQSSDSLLANVFRIALASL
                     EAEPVAWKATFTQIDHEYNTFTAMYSDKAEAERWVRLHKVGDFRAEITPLYAAPPAMV
                     VPDEMDLLTCHLDGVTETYADGWNACRAAMLKGDK"
     gene            5521..5886
                     /locus_tag="M2A35_004886"
     CDS             5521..5886
                     /locus_tag="M2A35_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201066.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPARLPGGFTIEDAKELHENLVRSHISQALSGEKMKKKDREADLRWIHDVIVQAA
                     WFVKASLEALPEPPQEVNQ"
BASE COUNT         1638 a         1256 c         1664 g         1472 t
ORIGIN      
        1 aagttcatca tcggtatagt ctggcgcgat gatgacgggt tctcgtttct gcatactgat
       61 tcctcgcggt gctgcttcgc ttatcagccg ttagattttg ccgagctgga aagcgcctgt
      121 ttaaactcac tgaagctgag agcttcttcg ccttcggcaa ggccttcgaa gtattcttcg
      181 taagcctttt ccatgattgt gtcgaaatcc atatcactca cctgagtttc tttccagcca
      241 gcgacgggca ccattttcgg ttttaaacgt tttgcttttg gtatacgtca tcgcggtgaa
      301 tgtgccgtcc tggttgggaa acacgccgta caccagagat tcgttgttgc caagaccgat
      361 agtatccatg ttgacctcat ttccccttaa cgccggagta gcggaactgt ttgctgagaa
      421 caccgtgcgg tgtcttgatg agtgaaattt agaataacct aaggcatatg gtcaagattt
      481 ttatgtagaa aaacctaagt tttttggtgt aaaaaacaca agtgtttgaa agtttgtgct
      541 ttttattaca gggtgtggag aaaaaagggg ttatttgttt gcgcttcttt tgcgagcttt
      601 aagtagttct tcaaaaagtt tgttgaaatt ttcaactcga gcacgcatct ctgacaacag
      661 ggccttttgc tctgagtcag gcagtgcgtc gaacagttga agtaactctt tttgatcttc
      721 tgtcagaatg gctggctgat tattcgggat cggttcgcct ggttgtttat cttcatcccc
      781 aaaaagaagc caagtcggtg agcactgaag cgcttggctc agtgcgaata atcttttccc
      841 cgccggctgt gtttcatctc tttcccattg agaaattgtt acgtgagcca ctttgaccag
      901 cttacctaat gcggcctgag acagttttaa ttttttacgc ctatgtaaga ggcgagcacc
      961 gaaggtttcg tttttcatat tagggaattc taatttttct tgacttaggt ttctctacga
     1021 tctagtttcc ttaggaaaat ctaaggggct cgatatgttg aaaattgatg ctatagcgtt
     1081 ttttggcagc aaaacaaagc ttgccaatgc cgcaggagtt aggttggcaa gcattgctgc
     1141 atggggggaa ctggttcctg aaggtcgcgc gatgcgcctg caagaggcat ccggcgggga
     1201 acttcagtac gaccccaaag tttatgacga atatcgtaag gcaaagcggg cggggcggtt
     1261 gaacaatgaa aatcacccct gaacaggttt gtgaggctct ggatgcctgg gtatgccgac
     1321 caggaatgac acaggagcag gcgacgatat taatcacgga agcattctgg gctctgaaag
     1381 aacgcccgaa catcgatgtt caacgcgtca cgtttgatga tggcgcggtt gatcaacggg
     1441 cgctgggcgt taaccgggtg aagatattcg aacgctggaa atctatcgac accagggata
     1501 agcgggaaaa attcacggcg ctgattccgg caattatggc ggctatccgg atcagtgatt
     1561 tcaggttgta tcgtgaaatt actgacggaa aaagcattac gtacatgatc gccgggttaa
     1621 acaaagaata tggcgatgtg gtggagtcag ggctactttt tgcggatcca gctgttgtgg
     1681 aacgtgagac tgacgaactt atagaaaaag ctattgcttt caagcgtgcg tatcgtcagc
     1741 aataccaaca ttactttgca gataaacaaa tatctgtctg gggttcgtat gagtatcgat
     1801 gcgctacgat gggctaaaaa ggtgaaaacc ggcagttcat ccagtaagtc agtattgacc
     1861 tggcttgctg atatgtgcgg tgccgatttg tgtgcatacc cgtctgtatc tgcactggca
     1921 gaagtaacgg aactgaacaa aaagactgtg caggacagct tacgacacct gatggagatt
     1981 gggttaattg ttgataccgg tgagagaaaa ggccggacaa aacaaattgt ggtgtaccga
     2041 cttatcggtg ttgaagaaag tgttaccgag gatgaataca cccaaaaacg ggagtcttta
     2101 aaggtaggta aaattggtac tgttaataaa aacagaaccg aaaacggtta tgtttcagca
     2161 caaaacagcc ccaaaaacgg aactctttgc tgcatggaaa ataaccaaag acacccaaat
     2221 tttccatcaa agacacccaa aaacggatca cggaacccaa aggaacccaa agatttaaac
     2281 cccacacata acgcacgcga gagtgctccg accagtgagc aggaagtttt gtcgttacag
     2341 gcagtccccc ctgtattcct ggatggcctg agcgaaccca tcggaaaatt tccgatgacc
     2401 gataactggt atccgtcacg ggattttcga cgacgggctg cgttgtgggg gatggctttg
     2461 ccggagacag aattcacacc tgctgaactt gccgcattcc gggactactg ggcagcggag
     2521 gggaaagtgt ttacgcaggt tcagtgggag cagaaattcg cccgtcacgt aaatcacgtc
     2581 agggcgcagg ttaaaccagt cagcaagggg gtaaaccatg cagcagcacc aggtggaacc
     2641 gcatcacggg cagttcagga aattcgggca gcacgtgagc agtgggaacg tgaaaacgga
     2701 tttatcagcg acggaaacgg cctggaagct gtgggagctc atgggggagg tttattcgaa
     2761 ccgctggacc cagaagaacg gggccgcacc ttcgaagctc tggattgcac agattggcgc
     2821 gatgactgag cagcaaatcc ggcaggtctg ccgccagtgc atggactgct gccgggcggg
     2881 tgaaacatgg cctccggacc tggctgagtt tgtggcgctg atttcggaaa gcggggccaa
     2941 tccatttggt ctgacggtgg atgctgtgat ggaggagtac cgccgctggc gcaatgagtc
     3001 ctggcgatac gacggaagtg ataagtaccc gtggtctcag cctgtgctgt atcacatttg
     3061 cctcgagatg cgttcaaagg ggattgagcg ccagatgacc gaaggggagt taaaacgact
     3121 tgcagaacgg caactagcga aatgggcaaa gcatgttggt gacggcttca gcgttccgcc
     3181 cgtacggcgg caactggcag caccaaaacg cccgtcgggg ccaacaccaa ttgagttgct
     3241 gaaacgggag tatgaacgcc ggaaggcagc tggttttgta tgaattgtga aggtgatttt
     3301 tcaggaggac ttgtggcaaa agtttttaca tccgaacagc gggaagagct aaaggcacga
     3361 attatcgggt tggtacgcaa aaatgaacgc atgacgatgt cgcaactgga gagagcgacg
     3421 ggagctggct ggcattcggt ccgacgctgc cttgtggatg tactggcttg tggtgattta
     3481 tatatgtccg gggaatacgg tgtttttgca tcagagcagg cgtatcgtga atggcgtaag
     3541 acaccggaga aaacaactga ccagacactg attcgaaagt taccagacgg agaaatacgc
     3601 cgctacgaca gacacctgaa cataatctgt cgcgagtgcc ggaagagtga ggttatgcag
     3661 cgagtgttgg cgttttatca gggtaatttt caggaggtga tggcgtgagg gtgagagtct
     3721 atatcgccgg tccaatgacc ggatataaaa atttcaaccg tgaggcgttc cacaaggcgg
     3781 aagaggaact gaaacgggaa gggcatacag tcttaaaccc ggcagtactt ccggacggac
     3841 tgacacagcc acactacatg gatatttgca tggcaatgat tcgttgtgtg gatgcgattt
     3901 acatgctgaa aggctggcag cggtcagcag gtgctaaggc agaactggcg ctggcggaga
     3961 aactggggca tgcagttatt ttccaggagg caaacagtga gtaaccaatg gcgaccagat
     4021 atttgtccta taaccggacg tgcatttttc atgtggattg agcatccgac attgggaaat
     4081 gtgccgacgt atggcggccc attagacagt tacaccattc caacaaagga cagcgatggt
     4141 gagttttcgt gtgagcgtta cgatcatgat ttcggtggct gggtagaaag cgaatgtctt
     4201 gggttatatc tgattgatga taaagaacaa tgcagagtct acgaactcga ggaacgtgtt
     4261 aaagagctgg aagcgcggga agttcatttg ccgactcgct acggccttcg atatggacat
     4321 ccgataaatg atgatgagcg ccacgtcatg atacctaaag aaaatggctg ctggctttat
     4381 ctggctgact tagaacatgc actacgtgtt gctggcattc acatcaaagg agaggagtat
     4441 gggaataaaa ccagaagatg agttaagtaa tattgtttta tttccggtaa aagaggatga
     4501 cccgcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt gtcatcacgc
     4561 ttctgtccag gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcagttgt
     4621 ggagccgttt gactggatgc tctctgtggc taaaagagaa accagactgg cagatgatgt
     4681 aaggttattg cgccaggagg agcaagaaag gcggaaaaat atagaaaagt taattcagat
     4741 tgagcgtaac gcgaaagcgc ggatacgtag ggcgacaaaa tccagaactg aataattaaa
     4801 tttagcgctg ttaaaaattt aatccttaac cggagggatt tctgcaccct caaaacatca
     4861 ggaggccgcc cgaaagggcg gtaataaatg actgcattat tcagaaaaga atatccgcga
     4921 aaaagtagag cgacagaatt tttgtttctc attctgttta tcgtgttgat gataccgata
     4981 tccccgctaa tttttgtctg ggtaatcggg aaaataattg agccagttat tgaattgtat
     5041 aacgacgtgg tatgggcctc gttcaacaca ctgcacaata aaattaatcc gtataaggag
     5101 aactgatatg atcactattt tcagagaaaa cgcggagatt aaatcattca tcactggttt
     5161 cctgagcgac gcggcgcacg ataaccaatc ttcagacagc ctgcttgcta atgtgtttcg
     5221 tatcgcgctg gcatcgctgg aagcagaacc ggtggcgtgg aaggcaacct tcacgcaaat
     5281 tgaccatgaa tataatacgt tcaccgctat gtattctgac aaagcagaag ccgaacgctg
     5341 ggtgcgactg cataaagtcg gtgattttcg ggcagaaata acaccgcttt acgcagcgcc
     5401 gccagcgatg gtagtgcctg atgaaatgga tttgcttacc tgccatctcg acggtgtaac
     5461 tgaaacatat gctgatggtt ggaacgcctg ccgcgccgcc atgcttaagg gagataaata
     5521 atgattaatc gaaccaaact ggagcacatt ctcgaatatg ccaggcagca gaaatgtatt
     5581 gggcaacttt gtaaaattcc accaggagat atggttgaaa tcgtggaaat ggccatgcgt
     5641 aaggctggca actctccggt agctccggct cgcctgcctg gtggtttcac cattgaggat
     5701 gcgaaggaat tacatgaaaa cctggttcgc agccacataa gccaggcttt aagtggcgaa
     5761 aagatgaaaa agaaagatcg cgaggctgat ttgcgctgga ttcatgacgt aatagttcag
     5821 gccgcgtggt ttgtgaaagc atcactggaa gcgttgcctg aaccaccgca ggaggtgaat
     5881 caatgacctg gcctgaagca ttcacaacgg taggaattgc gatggcggtg gcgctggtgg
     5941 tgtattcgat ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa
     6001 ttgctgcggg tgcttgaggc tatctgcctc
//
LOCUS       DAHBHC010000163         6000 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHBHC010000163 DAHBHC010000000
VERSION     DAHBHC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.167"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1046)
                     /locus_tag="M2A35_004887"
     CDS             complement(<1..1046)
                     /locus_tag="M2A35_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCB7201067.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKT"
     gene            complement(1043..2440)
                     /locus_tag="M2A35_004888"
     CDS             complement(1043..2440)
                     /locus_tag="M2A35_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCB7201068.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2595..3593
                     /locus_tag="M2A35_004889"
     CDS             2595..3593
                     /locus_tag="M2A35_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCB7201069.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3704..4609)
                     /gene="ompG"
                     /locus_tag="M2A35_004890"
     CDS             complement(3704..4609)
                     /gene="ompG"
                     /locus_tag="M2A35_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCB7201070.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPEVEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(4654..5736)
                     /locus_tag="M2A35_004891"
     CDS             complement(4654..5736)
                     /locus_tag="M2A35_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7201071.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(5750..>6000)
                     /locus_tag="M2A35_004892"
     CDS             complement(5750..>6000)
                     /locus_tag="M2A35_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCB7201072.1"
                     /translation="ADASQLKNSKPDPEIFLAACAGLGVPPQACIGIEDAQAGIDAIN
                     ASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQNV"
BASE COUNT         1432 a         1670 c         1437 g         1461 t
ORIGIN      
        1 gttttgcctt tttgcagcgt ttctttcacc tgaccgataa gctgacgacg gaaatccccg
       61 aggcgaggtt tgtcatcgtc aatccacggc agcgggcggc aaagctccat agctttaatc
      121 ccgagtcgtg ccgtcagaag tccagcacca attccctgag ctgcgcgggt agacaaacga
      181 gcagcgagat cttgcgacat ccagtccatc cccacttcgc gcaccagttc gctggctccg
      241 gcaaaagcga tattcagcaa taccagctta aacagacgca aacggctgta ataccccagt
      301 tcaatgccat acagcgtggc gatgcgatta attaaacgca gattgcgcca ggcgataaac
      361 gccatatcga ccaacgccag cgggctgacc gcaatcatca acgttgattc cgccgccgaa
      421 cggctgattt cgcgccgcgc ctgggcatct aaaactggct ggaccaaatg cgcatacaaa
      481 ctgaccactt cacggtcgtt ttgcgtttca tggattgagg cataccagcg ttgcagcgct
      541 ggatgagact gatcaatacc cgcctgctgc gccagttttt cgcaaaatgc gcggcctttg
      601 cccgtgccgt ggctgtgcaa caaatcgcgc gcttcgtcgc gttcatgggc gcgctgtcgc
      661 aagcgccata agcgccgcca ctctgttacc acagaaccta cgccagcgcc gataatcaat
      721 gccccagcgg cacatccacc cagcgccacc cagtcctgag tttgccaggc attcattgtc
      781 cactgtacac cctgcccgac aacgcttgcg ccaaacagag ccagcccgcc catcaccatt
      841 ttgcgccaca ggctgcgttt cggacgtaac gctgcatcca ttaccgcttc aacttgcccc
      901 tcttcttcag gcgcttcatc gagcgtggcc ggggcaaaat tttgcgcctg attttcgtca
      961 aaggtctgct gcgccctgaa ttttggattc tgatcgacct ccagcggacc gtcgaaatca
     1021 atacgtggtt ttaacggttc ggtcatcgca atttatctcc tattaaaaat tcaagcgcgg
     1081 catcaagacg aatatgcggc agcggtttgt cgacatccat cacctgcggg cgaaacgctt
     1141 caaactggaa cccttgcttg tcccagaacg cctgaccggg caaacgcgca ggaacttcac
     1201 cgggataaac ggtgagtggc gcaccatcgc tgagccggtt accgcgcaat gccggaattt
     1261 tctcgccgtt gacgtcaata atgccgctgg tggtcgcctg caccgaggcc agccccaggc
     1321 aatccatact aatcccttca aacgccgcat tttgccaggc atcctgaatc agttgttgca
     1381 gtaatgaaac catattggcg tgctgatcga tggtcacatg gtccgcttta gtggcagcaa
     1441 acaatagctt atcgataacc ggcgaaaaca aacgccggaa caaggtacgc tgcccgtagt
     1501 ggaaactttg catcagctgc gtcagtgcca gacgcatatc attaaatgcc tgtggcccac
     1561 tgttgagagg ttgcaggcaa tcaaccagca caatctggcg gtcaaagcgc agaaaatgat
     1621 tcttatagaa ccccttcacc accttctcgc agtaataatt aaaccgctcg cgcagcattc
     1681 cggcattggt gtgcttatcg gcctgcgcca gtttggactc gccccaggca tcgacatccg
     1741 gccacgggaa gaattgcagc gcgggcgcac ctgccatatc tcccggcaag acaaagcgcc
     1801 caggctgaat aaagtgcagc ccctgctgtt tacagtggtg gagataatcg gtccacgcgg
     1861 cggcaatgtc tgccagccgg ttttcgtcgg caggcgctag cgggtccagc ccttcgcaca
     1921 tcattcgcca tttgaccgac cattcgccgc gctgaccatt gagtaagccc gtcatctggc
     1981 gtgaccagct taaatagtcc tgcgccagca tcggcaggtc gagcaaccat tcgccgggat
     2041 aatccacaat ctccagatac agcgtggagg tgtccttgaa gtggcgtagc agcgaatcgt
     2101 tcgatttaaa acgtagcgcc aggcggattt cactgacgcc gcgcgttggc gtcggccagg
     2161 cgggaggatc gccatataac tgcgccagtc cttcatcata tgtgaagcgc ggaatgccaa
     2221 agtcacgctg aggaatgcgt tttacgccca gcaggcgctc ttcacgcacc gcgcttaaca
     2281 gcggcaagcg tgctccggcg tgaatattga gcaactgatt gaccatcgca gtgataaacg
     2341 ctgttttgcc gctgcggctt agtccggtta cggcgaggcg cagatgtctg tcgacacccc
     2401 gattcaccag cgcattaagt tcatttttaa gtcgcttcat tgctgtcctg tgctgctctg
     2461 aagaccagaa aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca
     2521 actgcccgtg ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac
     2581 ggagttcctg acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa
     2641 aatccaccgt atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa
     2701 aagtgttacg ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga
     2761 cctcttcggg ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg
     2821 gtaatccgtt tttctctgaa gttttgcatg ccatcaccgc caaagctgaa gaggaaggtt
     2881 tcgacgtgat attgcagacg tcgcacaatc cggcagaaga cttacaaaaa tgtgaaagca
     2941 aaattaagca gaaaatgatt aaaggcatta ttatgctaag ttcgccagcg gatgagtcat
     3001 tttttgccca acttgataaa tacgatattc ctgtcgtggt gattggcaaa gttgaaggtc
     3061 aatatgccca tgtttattct gtcgataccg ataattttgg cgacagcatt gcgttgaccg
     3121 atgcattaat tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc
     3181 atgtttcagt ggatcgggta aatggttata agcagagcct ggctgcgcat aatattgcag
     3241 tgcgtgatga atggattgtt gacggcggtt atacccatga aacagcactg caagccgcgc
     3301 gggaattatt aagccagtca ccgttgcctg aggcagtgtt tgctactgac agcctgaaat
     3361 taatgagtat ttatcgtgcg gcagcagaga aaaatattgc tattccgcag cagttagcgg
     3421 tggtgggtta tagtaatgaa acgctgtcgt ttattttaac gcctgcgcca ggcggtattg
     3481 atatcccaac acaggagtta ggtcaacaaa gctgtgagtt attattccgc ttaatttctg
     3541 gaaaaccgtc accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag
     3601 ctaccttgcc tgaatgccgg atgcgatgct agcgcatctt atccggccta catttatagt
     3661 tcggataaag tactcgtgcc gcatccggca atttagccca ttatcagaac gagtaattta
     3721 cgccgacacc tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa
     3781 acgcgtattc cagcgaaacg gaaagcccgt tctggaaatc ataaccgtaa aataaaccta
     3841 cacggttaaa atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactccagc
     3901 gatccagccc aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat
     3961 aggcgcgaat ttcttgcgta gaaaactcac cgttattgcg gctgtcgtcc atattaaagc
     4021 cgcgctcgag ataatagttc actcgcaagg caaccgtttc gttgaaggta tattgcaggc
     4081 ctgtttccgt ttcgactcgg gtatcagcgt aaccggtcgt gttcagatcg ttggcaaatt
     4141 tatacatcga caaccaaccg ttgaaacgta aatcgtcagt cagtttcaca tcccagtctg
     4201 gcgcgatttt ccagcgctgc atattcgccg tgtctttacc cggttcatca acgtagtgat
     4261 aaccataatt acggaaaccg ccggtcaggc cgaaactgaa atcatcgttt tcgaggaact
     4321 gataatgcac ctccacctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat
     4381 ctaccggccc ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa
     4441 aatagactga aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta
     4501 tttcgtacat cgcgccgata ttaaagtgcc agtcgttcct ttcctcggcc tgtgcgcagg
     4561 ccattcccgc acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt
     4621 gtattattct gggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc
     4681 atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata
     4741 atcatttaac gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat
     4801 aaattccgca ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg
     4861 cgcgtccgga tgaatatctt ccggacgtat tcccatcacg atgggcttat gcaaacttcc
     4921 ctgtgttttc agaaccgcta atttctcttc gggaatggtt aatttaagcg tttccgtaac
     4981 gaatttatcg ccatcgatcg tgccacgaat aaaattcatc gccggtgatc caataaatcc
     5041 ggcaacaaac atattcgctg gttggttata aaccgttttc ggcgcaccaa cttgctgaac
     5101 aatcccatcc ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg
     5161 ggtcacgtag atcatggtgg tattcagctt ctggtgtagc ttgctgatct ctgcgcgcat
     5221 ttgcacgcgc agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc
     5281 ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc
     5341 cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc
     5401 atcaatcacc tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac
     5461 cgtcatatgc ggatacaacg cgtagttctg gaacaccatc gctatattgc gtgctttggc
     5521 tggaacgtca ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag
     5581 cccggcaatc atgcgcaggg tggtcgactt accgcagccc gacgggccga caaacacgat
     5641 gaactcttta tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta
     5701 gattttttga atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt
     5761 gccagaaggc cgataaccgc gaccaggtca gagagtccgt tgaaggcaac agtaattgcg
     5821 ccccggttaa gcccgcgccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa
     5881 tgcccgcctg cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac
     5941 aggcggcgag aaagatttcc gggtccggtt tcgagttttt aagttgggaa gcatccgcgc
//
LOCUS       DAHBHC010000164         5970 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHBHC010000164 DAHBHC010000000
VERSION     DAHBHC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.168"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            17..439
                     /locus_tag="M2A35_004893"
     CDS             17..439
                     /locus_tag="M2A35_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201073.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            517..1305
                     /locus_tag="M2A35_004894"
     CDS             517..1305
                     /locus_tag="M2A35_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201074.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAGSVTQMDQKALPEEEEEEDINTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            1312..2058
                     /locus_tag="M2A35_004895"
     CDS             1312..2058
                     /locus_tag="M2A35_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCB7201075.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIAK"
     gene            2081..2842
                     /locus_tag="M2A35_004896"
     CDS             2081..2842
                     /locus_tag="M2A35_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCB7201076.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PEGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRHVASEK"
     gene            2858..3319
                     /locus_tag="M2A35_004897"
     CDS             2858..3319
                     /locus_tag="M2A35_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCB7201077.1"
                     /translation="MAKVFTQEEREKIKGKVVELVHQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDLYNSGYGLFPSEQARKDWIKARKKMSKAAVKKKSDPDLVYSLPDGEI
                     RRYDRRLNIICRECRRSEAMQRVLAFYQNGFREIFGEQVMHEGNHGTQVSV"
     gene            3556..4722
                     /locus_tag="M2A35_004898"
     CDS             3556..4722
                     /locus_tag="M2A35_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCB7201078.1"
                     /translation="MISAMLSNVKIFMNLQKRRKVQKLNYRGQVSKLKRGIFNSLKST
                     DWEGVENAKRKLEYYKRTRKLRVSKKKLTTSNKRVVFSAPISINYYNDRDFEIMNKFL
                     NNLRDCVLKNNRVYIDFSSTKYISAAAMLSFLAEVDVLIKKSEFGVNAIGFSHPKDKK
                     IESILNQVGFYDLLRKPKREIESYDDVTFWKYTSGSCSEPLLAKEMMVEIKKELERKS
                     SKKLYRGFTEAMSNSVEHAYVDDLMHTEDDETAKWWTFAGIHDKNLTVVICDKGVGIP
                     STLPKTQGVSVLMNIFKKLRVPLANVKDSTYIKASTLLQETRTGELNRGKGLNDIKSV
                     IDSIGDGFMGIFSSKGRYIYKGKTGIINEVLRDYKSSVNGTIIEWTIPCEVETE"
     gene            4724..5038
                     /locus_tag="M2A35_004899"
     CDS             4724..5038
                     /locus_tag="M2A35_004899"
                     /inference="COORDINATES: protein motif:HMM:NF025574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HCB7201079.1"
                     /translation="MKIIKVASRYPCPGPRFKRLGPASGEEFREWVERELKHSPDLTI
                     DLDGTEGYGSSFLEECFGGLIRRGVSPDKVKGIKFISEEEPELIDEINEYIEDAIKVS
                     HG"
     gene            5031..5549
                     /locus_tag="M2A35_004900"
     CDS             5031..5549
                     /locus_tag="M2A35_004900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201080.1"
                     /translation="MANSSIQVCTWSADFWCKFFSLFKDENQYVTWFLVLVGWGITAY
                     IAYLQTTKSRGDAIKDAHNEWIGEFRQKLELLEDFALEFWAEDNNKEPTLALAKMSRE
                     VKSLTTIAKEIERAGGEKYKAKLFKELRQAMTYDSDVHNRPLRPDCMQIVRIRETCAS
                     LRSAYGRKSLTE"
BASE COUNT         1797 a         1096 c         1584 g         1493 t
ORIGIN      
        1 taggagcggt tgattcatga aaatcaggca tgagcacatc gaatcagtgt tgttagccct
       61 ggcagccgaa aaagggcagg cgtgggtcgc taacgcaatt actgaagaat atctgcgcca
      121 ggggggcggc gaattgcccc ttgtaccagg caaggactgg aacaatcagc agaatatcta
      181 tcaccgttgg ttgaaaggtg aaacgaaagc gcaaagggaa aaaattcaga aactgatccc
      241 tgcggttctg gcaattcttc cgcgcgagct gcgtcaccga ctctgcatct tcgataccct
      301 ggaacgccgt gcattactgg cggcgcagga agcgttgagt acggcaattg atgcgcatga
      361 tgatgcagtc caggccgttt accggaaagc acatttcagc ggtggtgggt cgcccggcga
      421 ttctgtcgta gtgcattgat tgaaattaat cgtgccggat tgttttgttc ggtatcagtt
      481 aaatgtaacg ctgcgagcgt tacaaggtga aaacaaatgg cttcaaactg gataaagctc
      541 gaggttatta cgccggataa gccggaaata ttcaggcttg ctgagattct gaatattgat
      601 ccagatgccg cattagggaa ggttattcgc ttctgggcat gggcggatca acaaatgata
      661 gacggtaatg cagattgtaa cgctcgcggc gttacaaaaa gtgcaataga tcgcatcact
      721 tttatggctg gttttgctga tgcgttaatt caggttggat ggctggtcga aaatgacggt
      781 gggctttctc tacctaactt tgaacgtcat aacgggaaaa gctctaaaaa acgggcggtt
      841 acaaacgagc gagttacaaa aatacgcgaa ctgaaacgaa aaggtaacgc tggcagcgtt
      901 acacaaatgg atcaaaaagc gttaccagag gaagaggaag aggaagatat aaatactgat
      961 ctccccctaa atccccctcg ccaaaaacga gcgtctaaaa aatttgagcc ggaggctatt
     1021 gagctgcccg attggttgcc ggaaacactc tggcatgagt gggtccggtt caggcaggca
     1081 ttgcgaaaac cgattcgaac ggagcagggc gctaacgggg cgatacggga gctggaaaaa
     1141 ttccgccagc agggttttac acctgagcag gtgattcgac acagtatcgc caatgaatac
     1201 cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca ggttaacacc
     1261 gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacaggc catgaaaaat
     1321 attgcgacag gaggcgttct ggagcgtatc cgcagactga ccccaccaca tgtaaccgcc
     1381 ccattcagaa cggttgcgga gtggcgcgag tggcaacttg ctgaaggcca gaaacgttgc
     1441 gaggagatca accgcctgaa tcgtcagttg cgggtggaaa aaatcctgaa tcgctctggc
     1501 atccagccgt tgcaccgcaa atgctcgttt gcgaattacc aggtgcagaa cgaagggcag
     1561 cgatacgcgt taagccaggc gaaatccatc gccgatgaac tgatgaccgg gtgtacaaat
     1621 tttgcgttca gcggaaaacc tggtaccggg aagaatcact tagcggcagc tatcgggaat
     1681 cgcctgctga aagacggtca gacagtgatt gtggttaccg tggctgatgt tatgagcgcc
     1741 ctgcacgcca gctatgacga cgggcagtca ggcgaaaaat ttttgcggga gctgtgcgaa
     1801 gtggatctgc tggttcttga tgaaattggc attcagcgcg agacgaaaaa cgagcaggtg
     1861 gtgctgcacc agattgttga tcgccggaca gcgtcgatgc gcagcgtggg gatgctgaca
     1921 aacctgaact atgaggccat gaaaacattg ctcggcgagc ggattatgga tcgcatgacc
     1981 atgaacggcg ggcgatgggt gaattttaac tgggagagct ggcgtccgaa tgttggtcag
     2041 ccaggaattg cgaagtaatt tttaccggga ggaaatttta atggagactg tttttgacgc
     2101 actgaaagca atgggaaaag ccacatccat agaacttgct gcgcgacttg atatcagtcg
     2161 tgaagaagtg ctgaacgaac tatgggaact gaaaaaggct ggttttgttg ataaaagcgc
     2221 gtacacctgg cgtgtggctg ataacaacgt tcagcaggaa cagccagcgc aggcagaact
     2281 gccggaagaa accaccacag caacagtagc gaaaatctca gagtgcgatt taaccgcaac
     2341 gattgaacaa cgcggaccac aaacggctga tgagctggct acattgtttg gtaccacatc
     2401 acgcaaagtg gcttcaacgc tggcaatggc aatcagcaaa ggtcgtctga ttcgcgtaaa
     2461 tcagggcggt aaatttcgtt actgcatacc gggcgataat ttaccagcag agccgaaagc
     2521 agcatcggta gcggaaactg atggtaaggc ctttcctcag cccgaaggtg ttgcattacc
     2581 agtacaggag gctgcaacac aggaagatat taaaacagaa acggtggcgg acattgtgca
     2641 gtcgctgcca tcgtttactg aaacgcgagc ggatgacctg gttttaccat cactgcatat
     2701 ggcaaaccgc gaactgcgtc gggcgaaaaa tcatgtccag aagtgggagc gtgtctgcgc
     2761 cgcgctgcgt gagttgaaca agcacaggga tattgtacga cagattactg attcttcccg
     2821 ccatgttgca tcggaaaagt gattgccgga ggcgcttatg gcaaaagtat ttacacaaga
     2881 agagcgggaa aaaattaagg ggaaggttgt tgagctcgtg caccagagtg ggcgcgagac
     2941 gttacgacaa ctggaagcta agacaggtgc gacaaggtat ctgatgagtg ttcttgccag
     3001 agagctggtt gccagtggcg atttatacaa ctccggctac gggttatttc cgtctgaaca
     3061 ggcacgtaag gactggataa aggcccgcaa aaagatgtcg aaagcagcag tgaaaaaaaa
     3121 gagcgacccg gacctggttt attcattacc agacggagaa atacgccgct atgacaggcg
     3181 tctgaacata atctgtcgcg agtgtcggag gagcgaagct atgcagcgcg tactggcgtt
     3241 ttaccagaat ggttttcgag agatattcgg tgaacaggtt atgcatgagg gaaatcatgg
     3301 cactcaggtc agtgtgtagg cacgttgacg gtagaaagca tatgggggat cgatcgttct
     3361 tgttatgctg taagagcgaa agcgcaggcg ctgggtatca acaggatgtt acggggtgac
     3421 tttcacttga gctagtggga tcaattttac tgttggggtg gggggacgtt aagttgctga
     3481 caatttcgaa atgtttttga taaaggcagc gattttcttt gatggagggg ctacaaaaaa
     3541 tttttcatgt gaaatatgat atctgcgatg ttatcaaatg tgaaaatatt tatgaatctg
     3601 caaaaaagaa gaaaagttca aaaactaaac tatcgcgggc aagtttcgaa actgaagagg
     3661 gggatattta attctctgaa aagcacggat tgggaaggag tagaaaatgc gaagagaaaa
     3721 ctggaatatt acaaacgcac tcgtaagcta cgagtctcga agaaaaagtt gacaacaagt
     3781 aataaaaggg tggttttttc tgctccgata tctattaatt attacaacga tcgtgatttt
     3841 gaaatcatga ataaattttt aaataattta cgcgattgcg tgttgaagaa taatcgtgtt
     3901 tatatagatt tttcttcaac taaatatata agtgcagctg ctatgttatc ttttctcgct
     3961 gaagttgatg tgcttattaa gaaaagtgaa tttggagtaa atgccatagg gttttcacat
     4021 ccgaaagaca agaaaattga aagcattttg aatcaagtcg gtttctatga tctattaagg
     4081 aaacctaaaa gagaaattga aagttatgat gatgtgactt tttggaaata tacatcaggt
     4141 tcttgttctg agcctctttt agcaaaagaa atgatggtgg aaataaaaaa agagttagaa
     4201 agaaaatcat caaaaaaatt ataccgtggc tttactgagg ccatgtctaa ttctgtcgag
     4261 catgcttatg ttgatgattt aatgcatacg gaagatgacg aaacagctaa gtggtggacc
     4321 tttgctggca tacatgacaa aaatcttaca gtggtaatat gtgataaagg agtgggaata
     4381 ccttcaacat tgcctaaaac acaaggtgta agtgtgctaa tgaacatctt caaaaaactt
     4441 agagttccat tagcaaatgt aaaggattct acttatataa aggcttccac gttacttcag
     4501 gaaacgagga ctggggagct gaaccgaggt aaaggcttga atgatatcaa atcggtaatc
     4561 gattcaatag gtgatggttt tatggggatt ttttcatcaa aagggcgcta tatttacaaa
     4621 gggaaaactg gcattattaa cgaagtgctt agagattaca aaagctctgt taatggtact
     4681 ataattgaat ggacaattcc ttgtgaggtg gaaactgagt agtatgaaaa ttataaaagt
     4741 agccagtcgt tatccttgcc caggacctcg ttttaaaaga ttggggcccg cttctggtga
     4801 ggagtttcgt gagtgggtag aaagagagct taaacattca ccagatctaa caatagacct
     4861 tgatggaacg gaggggtatg gttcttcttt cttggaggag tgctttggcg gattgattag
     4921 gcgtggcgtg agtcctgata aggttaaagg gattaaattt atctcagaag aagaacctga
     4981 gttaattgac gagataaatg aatatatcga ggacgctata aaggtatctc atggctaatt
     5041 caagtatcca ggtctgtact tggagtgcag acttttggtg taagtttttt tctttgttta
     5101 aagatgaaaa tcagtatgta acttggtttt tagttttagt tggatggggg attacggcat
     5161 atattgctta tctccaaacc acaaaaagta gaggagatgc tattaaagat gctcataatg
     5221 aatggatcgg tgagtttaga caaaaactgg agttgcttga ggattttgcc ttagagttct
     5281 gggctgaaga taataataaa gaacctacac tggcgttagc aaaaatgagt agggaggtta
     5341 agagtttaac tactatcgct aaagaaattg aaagggccgg tggtgaaaaa tataaggcaa
     5401 aattgtttaa ggaattacgc caagccatga catatgatag tgatgttcac aaccgacctc
     5461 taagacctga ttgtatgcaa attgttagga ttagagaaac atgtgcaagt ttgagaagtg
     5521 cgtacgggcg caaaagcttg actgaataac ctcatattgc tgagtagctg gtactgcata
     5581 ttattatgaa aacacatcgt ggtttttata taaaagctgc ttattattaa gagatctgtt
     5641 ttatgttttt ggctagtaaa atgtgattct taacttaaat gttcccttac actagattta
     5701 taatatcatc gtgtgattta taaactatgc attagtaata attaattttt cggaaaaata
     5761 gttaagtaaa attgctgcgt gtgcttgagg ctatctgcct cgggcatgaa caccaacggc
     5821 agatagagaa aagcccagtt aacattacgc gtcccgcaag acgtttaata ttaaacttag
     5881 gccaatttca tgctagacac atggatgtta gcctcttacg tgccgaaagg caaggagaag
     5941 caggctatga agcagcaaaa ggcgatgtta
//
LOCUS       DAHBHC010000165         5959 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHBHC010000165 DAHBHC010000000
VERSION     DAHBHC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.169"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..315
                     /locus_tag="M2A35_004901"
     CDS             <1..315
                     /locus_tag="M2A35_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCB7201081.1"
                     /translation="EDNAAQLEETWMSVARFPDLTVSHMQKSKFSYIENECGIKIIGT
                     FETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLYSNIFEFQVKYFF
                     PR"
     gene            complement(360..1259)
                     /gene="eamA"
                     /locus_tag="M2A35_004902"
     CDS             complement(360..1259)
                     /gene="eamA"
                     /locus_tag="M2A35_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCB7201082.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGACTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1454..2641
                     /gene="ydeE"
                     /locus_tag="M2A35_004903"
     CDS             1454..2641
                     /gene="ydeE"
                     /locus_tag="M2A35_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCB7201083.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            2768..2863
                     /gene="mgtS"
                     /locus_tag="M2A35_004904"
     CDS             2768..2863
                     /gene="mgtS"
                     /locus_tag="M2A35_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCB7201084.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3082..3972)
                     /gene="dgcZ"
                     /locus_tag="M2A35_004905"
     CDS             complement(3082..3972)
                     /gene="dgcZ"
                     /locus_tag="M2A35_004905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCB7201085.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4227..4619)
                     /gene="ydeI"
                     /locus_tag="M2A35_004906"
     CDS             complement(4227..4619)
                     /gene="ydeI"
                     /locus_tag="M2A35_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCB7201086.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGALKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            4895..5413
                     /locus_tag="M2A35_004907"
     CDS             4895..5413
                     /locus_tag="M2A35_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCB7201087.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(5457..>5959)
                     /locus_tag="M2A35_004908"
     CDS             complement(5457..>5959)
                     /locus_tag="M2A35_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HCB7201088.1"
                     /translation="ARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDM
                     RWHCLEENEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLW
                     TQMLADDGYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQ
                     HRGLNI"
BASE COUNT         1613 a         1457 c         1289 g         1600 t
ORIGIN      
        1 gaggataatg cagcacaatt agaagaaacg tggatgtcag tagcacgttt tcctgattta
       61 accgtgtcac atatgcaaaa atccaaattt tcatatattg agaatgaatg cgggatcaaa
      121 atcattggca cctttgaaac tttctcccct acttttccta ccccagaaat cgccagtatt
      181 ttacggatca gcccacggga tcccatactt aaaattcaga cccaggctgt ggacagtaac
      241 tctattccgc tggattattc gttactttac agcaatattt tcgagttcca ggtaaagtac
      301 tttttcccgc gataaaagca gacgcacaga gtgctgacat cacattctgt gcgtatggtt
      361 tagcctctaa cctttaccgc tttacgccac cgcaagccaa atacattgat atacagcccg
      421 gtcataatga gcaccgcacc taaaaattgc agacccgtta agcgttcatc caacaatagt
      481 gccgcacttg ccagtccgac tacgggcacc agtaacgata acggtgcaac ccgccaggtt
      541 tcatagcgtc ccagtaacgt cccccagatc ccataaccaa caattgtcgc cacaaacgcc
      601 agatacatca gcgacaagat ggtggtcata tcaatagtaa ccagactgtg aatcatggat
      661 gcggaaccat cgagaatcag cgaggcaaca aagaagggaa tgattgggat taaagcgctc
      721 cagattacca gcgacatcac cgccggacgc gttgagtgcg acatgatctt tttattgaag
      781 atgttgccac acgcccaact aaatgctgca gccagggtca acataaagcc gagcatcgcc
      841 acatgctgac cgttcagact atcttcgatt aacaccagta cgccaaaaat cgctaaggcg
      901 atccccgcca attgtttgcc atgcagtcgc tccccgaaag tacacgcgcc aagcacgata
      961 gtaaaaaacg cctgtgcctg taacaccagc gaagccagtc cagcaggcat accaaagtta
     1021 atggcgcaga acagaaaggc aaactgcgca aaactgatgg ttaatccata ccccaagagc
     1081 aaattcagtg gtactttcgg tcgtgcaaca aaaaagatgg ccggaaaagc gaccagcata
     1141 aagcgcaaac cggccagcat cagcggtggc atgttatgaa gccccacttt gatgaccaca
     1201 aaatttagcc cccatacgac cactaccagt agcgccaaca ccccatcttt tcgcgacatt
     1261 ctaccgcctc tgaatttcat cttttgtaag caatcaactt agctgaattt acttttcttt
     1321 aacagttgat tcgttagtcg ccggttacga cggcattaat gcgcaaataa gtcgctatac
     1381 ttcggatttt tgccatgcta tttctttaca tctctaaaac aaaacataac gaaacgcact
     1441 gccggacaga caaatgaact tatccctacg acgctctacc agcgcccttc ttgcctcgtc
     1501 gttgttatta accatcggac gcggcgctac gctgccattt atgaccattt acttgagtcg
     1561 ccagtacagc ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc tcactattgg
     1621 cgtcgttttt agcctcggtt ttggtatcct ggcggataag ttcgacaaga aacgctatat
     1681 gttactggca attaccgcct tcgccagcgg ttttattgcc attcctttag tgaataacgt
     1741 gacgctggtg gtgctctttt ttgccctcat taactgcgcc tattctgttt ttgctaccgt
     1801 gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa tcttctcaat
     1861 caactacacc atgctcaaca ttggctggac catcggtccg ccgctcggca cgctgttggt
     1921 gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt ttcccatgct
     1981 tttcattcaa atttgggtaa agcgcagcga gaaaatcatc gccacggaaa caggcagtgt
     2041 ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct gctctggttt
     2101 tctggcttct tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat
     2161 tgctgatggg gattttgccg aaaaggtggt cgcggttgtt cttccggtga atgctgccat
     2221 ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac ccggctaaca tccgcgcgct
     2281 gatgacagca ggcaccctct gtttcgtcat cggtctggtc ggttttattt tttccggcaa
     2341 cagcctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc
     2401 gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag ccagctattt
     2461 ttccgcccag tctttaggct ggcttggtgc cgcgattaac ccattagtga gtggcgtagt
     2521 gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga tcattgctgc
     2581 gtgggtgctg atgttaaaag ggattcgagc aagaccgtgg gggcagcccg cgctttgttg
     2641 atttaagtcg aacacaataa agatttaatt cagccttcgt ttaggttacc tctgctaata
     2701 tctttctcat tgagatgaaa attaaggtaa gcgaggaaac acaccacacc ataaacggag
     2761 gcaaataatg cttggtaata tgaatgtttt tatggccgta ctgggaataa ttttattttc
     2821 tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg agataatccc
     2881 tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta tgacggcgaa
     2941 aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt
     3001 acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc
     3061 tcaatgaatg ttaaacggag cttaaactcg gttaatcacg ttttgttcgt caataaacat
     3121 gcagcgattt cttccggttt gcttaccctc atacattgcc cggtccgctc ttccaataac
     3181 cacatccaga ggctcttcag gaaatgcgcg actcacacct gctgtcacgg taatgttgat
     3241 atgcccttca gaatgtgtga tggcatggtt atcgactaac tggcaaattc tgacacctgc
     3301 acgacatgct tcttcatcat tagccgcttt gacaataatg ataaattctt cgcccccgta
     3361 gcgataaacc gtttcgtaat cacgcgtcca actggctaag taagttgcca gggtgcgtaa
     3421 tactacatcg ccgattaaat gcccgtaggt atcattaacc aatttaaatc ggtcaatatc
     3481 caacaacatt aaataaagat tcagaggctc agcgttgcgt aactgatgat caaaggattc
     3541 atcaagaacc cgacgacccg gcaatcccgt caaaacatcc atattgctac ggatcgtcag
     3601 caaataaatt ttgtaatcgg ttaatgccga agtaaaagaa agcaacccct cctgaaaggc
     3661 gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc agcattaatt cccgaccaca
     3721 gttatgcata tgttgatggg cagaatccat tagccgaacg taaggtaatt catcgttatc
     3781 gagtggcccc agatgatcaa tccaccgacc aaactggcac agtccataag aatggttatc
     3841 cgttatttct ggcttactgg catctctcgc gaccacgctg tgaaacatac tcaccagcca
     3901 ctggtagtgg gcatcgatag ccttattgag atttaacaag atggcatcaa tttccgttgt
     3961 cttcttgatc attgccactc ctttttcaca gttccttgtg cgcgctattc taacgagaga
     4021 aaagcaaaat tacgtcaata ttttcataga aatccgaagt tatgagtcat ctctgagata
     4081 acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt ttaattctgt aaatcacctc
     4141 tgcattatcg taaataaaag gatgacaaat agcataaccc aataccctaa tggcccagta
     4201 gttcaggcca tcaggctaat ttatttttat ttctgcaaat gagtgacccg aacgacggcc
     4261 ggcgcgcttt tcttatccag actgccgcta atgttgatca tctggtccgg ctgaacttct
     4321 cgtccatcaa agacggccgc aggaataacg acattaattt caccgctctt atcgcgaaaa
     4381 acgtaacggt cctctccttt gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca
     4441 tcgtgcatgt tttttgcgaa atcaacggtc attttttttg catcatcggt tccgcgatag
     4501 ccatcttcta ttgcatgagg cggcggtggc gctgcatcct gttttaaagc gccctggtca
     4561 tctgccaacg cataaggcat gacaagaaaa cttgctaata caatggcctg aaatttcata
     4621 ctgactcctt aattgcgttt ggtttgactt attaagtctg gttgctattt ttataattgc
     4681 caaataagaa tattgccaat tgttataagg catttaaaat cagccaacta gctgtcaaat
     4741 atacagagaa tttaactcac taaagttaag aagattgaaa agtcttaaac atattttcag
     4801 aataatcgga tttatatatt tgaaaattat tatattggac gagcatacag aaaaagcaaa
     4861 tcacctttac atataaaagc gtggacaaaa aacagtgaac attaatagag ataaaattgt
     4921 acaacttgta gataccgata ctattgaaaa cctgacatcc gcgttgagtc aaagacttat
     4981 cgcggatcaa ttacgcttaa ctaccgccga atcatgcacc ggcggtaagt tggctagcgc
     5041 cctgtgtgca gctgaagata cacccaaatt ttacggtgca ggctttgtta ctttcaccga
     5101 tcaggcaaag atgaaaatcc tcagcgtaag ccagcaatct cttgaacgat attctgcggt
     5161 gagtgagaaa gtggcagcag aaatggcaac cggtgccata gagcgtgcgg atgctgatgt
     5221 cagtattgcc attaccggct acggcggacc ggagggcggt gaagatggta cgccagcggg
     5281 taccgtctgg tttgcgtggc atattaaagg ccagaactac actgcggtta tgcattttgc
     5341 tggcgactgc gaaacggtat tagctttagc ggtgaggttt gccctcgccc agctgctgca
     5401 attactgcta taaccaggct ggcctggcga tatctcaggc cagccattgg tggtgtttat
     5461 atgttcaagc cacgatgttg cagcatcggc ataatcttag gtgccttacc gcgccattgt
     5521 cgatacaggc gttccagatc ttcgctgtta cctctggaaa ggatcgcctc gcgaaaacgc
     5581 tgcccatttt cacgcgttaa tccgccctgc tcaacaaacc actggtaacc atcatcggcc
     5641 aacatttgcg tccacagata agcgtaataa cctgcagcat atccgccacc aaaaatatgg
     5701 gcgaaataac tgctgcgata gcgtggcggt atagcaggaa gatccatatt ttccgccacc
     5761 agcgcccgca attcaaaatc atcgacatcc tgcattgctt cgttttcttc caggcaatgc
     5821 cagcgcatat cgagaagtgc ggcgctaagc agttcgctca tctcataccc tttgttgaac
     5881 aggctggcat tacgcatttt ctgttgcagt tcgtcaggca ttgctgcccc gctctgataa
     5941 tgccgggcgt agcgagcga
//
LOCUS       DAHBHC010000166         5882 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHBHC010000166 DAHBHC010000000
VERSION     DAHBHC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.170"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..525
                     /locus_tag="M2A35_004909"
     CDS             <1..525
                     /locus_tag="M2A35_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCB7201089.1"
                     /translation="TEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSF
                     KAVKEKLDTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENDVKKNFLPD
                     NTMVLGNTQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLM
                     LLADPDAFVSVQLA"
     gene            567..962
                     /locus_tag="M2A35_004910"
     CDS             567..962
                     /locus_tag="M2A35_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCB7201090.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            974..1348
                     /locus_tag="M2A35_004911"
     CDS             974..1348
                     /locus_tag="M2A35_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCB7201091.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1339..1917
                     /locus_tag="M2A35_004912"
     CDS             1339..1917
                     /locus_tag="M2A35_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201092.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRECLPKELGYALQHQLRMVIKR"
     gene            1914..2309
                     /locus_tag="M2A35_004913"
     CDS             1914..2309
                     /locus_tag="M2A35_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201093.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2317..3057
                     /locus_tag="M2A35_004914"
     CDS             2317..3057
                     /locus_tag="M2A35_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201094.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3073..3495
                     /locus_tag="M2A35_004915"
     CDS             3073..3495
                     /locus_tag="M2A35_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCB7201095.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3477..3911
                     /locus_tag="M2A35_004916"
     CDS             3477..3911
                     /locus_tag="M2A35_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCB7201096.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDIHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            3904..>5882
                     /locus_tag="M2A35_004917"
     CDS             3904..>5882
                     /locus_tag="M2A35_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7201097.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYKRLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWIAGLKSGWSEWEESATDSMSQVKSAATQTF"
BASE COUNT         1365 a         1477 c         1899 g         1141 t
ORIGIN      
        1 acggagtgga gcaagcgtga caagtccacg tatgacccga ccgacgatat cgaagcctac
       61 gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg atccgaaagg ctgggcgctg
      121 ttccgttcct tcaaagccgt caaggagaag ctggataccc gtcgcggctc tcattccgag
      181 ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct ataagggaat gtatggcgat
      241 gtggccatcg tcgtgtattc cggacagtac gtggaaaacg acgtcaaaaa gaacttcctt
      301 ccggacaaca cgatggtgct ggggaacact caggcacgcg gtctgcgcac ctatggctgc
      361 attcaggatg cggacgcaca gcgcgaaggt attaacgcct ctgcccgcta cccgaaaaac
      421 tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga ttcagtcagc accgctgatg
      481 ctgctggctg accctgatgc gttcgtgtcc gtacaactgg cgtaatcatg gcccttcggg
      541 gccattttct ctctgtggag gagtccatga cgaaagatga actgattgcc cgtcttcagg
      601 tgctgggtga gcaactgaac cgtgatgtca gcctgacggg gacgaaagaa gaactggcac
      661 tccgtgtggc agagctggaa gaggagcttg atgacacgga tgacgctgcc ggtcaggaca
      721 catctgtcag cccggaaaat gcgctgaccg gacatgaaaa tgaggttgta tcagcgcagc
      781 cggataccgt gactgatacg gctgatctgg tcacggttgt ggcactggtg acgctgcata
      841 ctgatgcact tcacgccacg cgggatgagg ctgtggcatt tgtgctgccg ggaacggcgt
      901 tccgtgtctc tgccggtgtg gcagctgaaa tgacagagcg cggcctggcc agaatgcaat
      961 aacgggaggc gctgtggctg atttcgataa cctgttcgat gctgccattg cctgcgccga
     1021 tgaaacgata cgcgggtaca tgggaacgtc agccaccatg acatccggtg agcagtccgg
     1081 tgctgtgata cgtggtgttt ttgatgaccc tgaaaatatc agctatgccg gacagggcgt
     1141 gcgcgttgaa ggctccagcc cgtccctgtt tgtccggact gatgatgtgc ggcagctgcg
     1201 gcgcggcgac acgctgacca tcggtgagga aaacttctgg atagaccgga tttcgccgga
     1261 tgatggcgga agctgtcatc tctggcttgg gcggggcgta ccgcctgccg ttaaccgtcg
     1321 ccgctggaag ggggatgtat ggccataaaa ggtcttgagc aggccgttga aaacctcagc
     1381 cgtatcagca gaacggcggt gcccggtgcc gccgcaatgg ccattaaccg cgttgcttca
     1441 tccgcgatat cgcagtcggt gtcacaggtt gcccgtgaga caaaggtacg ccggaaactg
     1501 gtaaaggaaa gggccaggct gaaaagggcc acggtcaaaa atccgcaggc cagaatcaag
     1561 gttaaccggg gggatttgcc cgtaatcaag ctgggtaatg cgcgggttgt cctttcgcgc
     1621 cgcaggcgtc gtaaaaaggg gcagcgttca tccctgaaag gtggcggcag cgtgcttgtg
     1681 gtgggtaacc gtcgtattcc cggcgcgttt attcagcaac tgaaaaatgg ccggtggcat
     1741 gttatgcagc gtgtggccgg gaaaaaccgt taccccattg atgtggtgaa aatcccgatg
     1801 gcggtgccgc tgaccacggc gtttaaacaa aatattgagc ggatacggcg tgaatgtctt
     1861 ccgaaagagc tgggctatgc gctgcagcat caactgagga tggtaataaa gcgatgaaac
     1921 atactgaact ccgtgcagcc gtactggatg cactggagaa gcatgacacc ggggcgacgt
     1981 tttttgatgg tcgccccgct gtttttgatg aggcggattt tccggcagtt gccgtttatc
     2041 tcaccggcgc tgaatacacg ggcgaagagc tggacagcga tacctggcag gcggagctgc
     2101 atatcgaagt tttcctgcct gctcaggtgc cggattcaga gctggatgcg tggatggagt
     2161 cccggattta tccggtgatg agcgatatcc cggcactgtc agagttgatc accagtatgg
     2221 tggccagtgg ctatgactac cgacgcgacg atgatgcggg cctgtggagt tcagccgatc
     2281 tgacttatgt cattacctat gaaatgtgag gacgctatgc ctgtaccaaa tcctgtaatg
     2341 ccggtgaaag gtgccgggac caccctgtgg gtttataagg ggagcggtga cccttatgcg
     2401 aatccgcttt cagacgttga ctggtcgcgt ctggcaaaag ttaaagacct gacgcccggc
     2461 gaactgaccg ctgagtccta tgacgacagc tatctcgatg atgaagatgc ggactggact
     2521 gcgaccgggc aggggcagaa atcagccgga gataccagct tcacgctggc gtggatgccc
     2581 ggagagcagg ggcagcaggc gctgctggcg tggtttaatg aaggtgatac ccgtgcctat
     2641 aaaatccgct tcccgaacgg cacggtcgat gtgttccgtg gctgggtcag cagtatcggt
     2701 aaagcggtga cggcgaagga agtgatcacc cgcacggtga aggtcaccaa tgtgggacgc
     2761 ccgtcgatgg cagaagatcg cagcacggtc acagcggcaa ccggcatgac cgtgacgcct
     2821 gccagcacct cggtggtgaa agggcagagc accacgctga ccgtggcatt ccagccggaa
     2881 ggcgcaaccg acaagagctt ccgtgcggtg tctgcggata aaacaaaagc caccgtgtcg
     2941 gtcagtggta tgaccatcac cgtgaaaggt gttgctgcag gcaaggtcaa cattccggtc
     3001 gtatccggta atggtgaact tgctgcggtt gcagaaatca ctgtcaccga cagttaatcc
     3061 ggagagtcag cgatgttcct gaaaaccgaa tcatttgaac ataacggcgt gaccgtcacg
     3121 ctttctgaac tttcagccct gcagcgtatt gagcatctcg ccctgatgaa acggcaggca
     3181 gaacaggcgg agtcagacag caaccggaag tttacagtgg aagacgccat cagaaccggt
     3241 gcttttgtgg tggcgatgtc cctgtggcat aaccatccgc agaagacaaa gcagccttcc
     3301 atgaatgaag ccgttaaaca gattgagcag gaagtgctta ccacctggcc cacagaggca
     3361 atttctcatg ctgaaaacgt ggtgtaccgg ctgtccggta tgtatgagtt tgtggtgaat
     3421 gatgcccctg aacaggcaga ggacgccggg cctgcagagc ctgtttctgc gggaaagtgt
     3481 tcgacggtga gctgagtttt gccctgaaac tggcgcgcga gatggggcga cccgactggc
     3541 gtgccatgct tgccgggatg tcatccacgg agtatgccga ctggcaccgc ttttacagta
     3601 cccattattt tcatgatgtt ctgctggata tgcacttttc cgggctgacg tacaccgtgc
     3661 tcagcctgtt tttcagcgat ccggatatac atccgctgga tttcagtctg ctgaaccggc
     3721 gtgaggctga cgaagagcct gaagatgatg tgctgatgca gaaagcggca gggcttgccg
     3781 gaggcgtccg ctttggcccg gacgggaatg aagttatccc cgcttccccg gatgtggcgg
     3841 acatgacgga ggatgacgta atgctgatga cagtatcaga agggatcgca ggaggagtcc
     3901 ggtatggctg aaccggtagg cgatctggtc gttgatttaa gtctggatgc ggccagattt
     3961 gacgagcaga tggccagagt caggcgtcat ttttccggta cggaaagtga tgcgaaaaaa
     4021 acagcggcag tcgttgaaca gtcgctgagc cgacaggcgc tggctgcaca gaaagcgggg
     4081 atttccgtcg ggcagtataa agccgccatg cgtatgctgc ctgcacagtt caccgacgtg
     4141 gccacgcagc ttgcaggcgg gcaaagtccg tggctgatcc tgctgcaaca gggggggcag
     4201 gtgaaggact ccttcggcgg gatgatcccc atgttcaggg ggcttgccgg tgcgatcacc
     4261 ctgccgatgg tgggggccac ctcgctggcg gtggcgaccg gtgcgctggc gtatgcctgg
     4321 tatcagggca actcaaccct gtccgatttc aacaaaacgc tggtcctttc cggcaatcag
     4381 gcgggactga cggcagatcg tatgctggtc ctgtccagag ccgggcaggc ggcagggctg
     4441 acgtttaacc agaccagcga gtcactcagc gcactggtta aggcgggggt aagcggtgag
     4501 gctcagattg cgtccatcag ccagagtgtg gcgcgtttct cctctgcatc cggcgtggag
     4561 gtggacaagg tcgctgaagc cttcgggaag ctgaccacag acccgacgtc ggggctgacg
     4621 gcgatggcac gccagttcca taacgtgacg gcggagcaga ttgcgtatgt tgctcagttg
     4681 cagcgttccg gcgatgaagc cggggcattg caggcggcga acgaggccgc aacgaaaggg
     4741 tttgatgacc agacccgccg cctgaaagag aacatgggca cgctggagac ctgggcagac
     4801 aggactgcgc gggccttcaa atccatgtgg gatgcggtgc tggatattgg tcgtcctgat
     4861 accgcgcagg agatgctgat taaggcagag gctgcgttta agaaagcaga cgacatctgg
     4921 aatctgcgca aggatgatta ttttgttaac gatgaagcgc gggcgcgtta ctgggatgat
     4981 cgtgaaaagg cccgtcttgc gcttgaagcc gcccgaaaga aggctgagca gcagactcaa
     5041 caggacaaaa atgcgcagca gcagagcgat accgaagcgt cacggctgaa atataccgaa
     5101 gaggcgcaga aggcttacaa acggctgcag acgccgctgg agaaatatac cgcccgtcag
     5161 gaagaactga acaaggcact gaaagacggg aaaatcctgc aggcggatta caacacgctg
     5221 atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa agccgaaaca gtccggcgtg
     5281 aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg ctgccctgct gacgcttcag
     5341 gcagaactcc ggacgctgga gaagcatgcc ggagcgaatg agaaaatcag ccagcagcgc
     5401 cgggatttgt ggaaggcaga aagtcagttc gcggtactgg aggaggcggc acaacgtcgc
     5461 cagctgtccg cacaggagaa atccctgctg gcgcataaag atgagacgct ggagtacaaa
     5521 cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg agcgcctgaa cgcgctggcg
     5581 cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac gggccgccat tgatgcgaaa
     5641 agccgggggc tgactgaccg gcaggcagaa cgggaagcca cggaacagcg cctgaaggaa
     5701 cagtatggcg ataatccgct ggcgctgaat aacgtcatgt cagagcagaa aaagacctgg
     5761 gcggctgaag accagcttcg cgggagctgg atagcaggcc tgaagtccgg ctggagtgag
     5821 tgggaagaga gcgccacgga cagtatgtcg caggtaaaaa gtgcagccac gcagaccttt
     5881 ga
//
LOCUS       DAHBHC010000167         5746 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHBHC010000167 DAHBHC010000000
VERSION     DAHBHC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.171"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..648
                     /locus_tag="M2A35_004918"
     CDS             <1..648
                     /locus_tag="M2A35_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCB7201098.1"
                     /translation="LGGYLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRARGRGWL
                     GSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHDSAQHVRAGFRLGS
                     HNDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNM
                     TFSSSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYNGQATLNVTF"
     gene            769..3285
                     /locus_tag="M2A35_004919"
                     /pseudo
     CDS             769..3285
                     /locus_tag="M2A35_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3361..3816
                     /locus_tag="M2A35_004920"
     CDS             3361..3816
                     /locus_tag="M2A35_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201099.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            3895..4128
                     /locus_tag="M2A35_004921"
     CDS             3895..4128
                     /locus_tag="M2A35_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCB7201100.1"
                     /translation="MAGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            4228..5046
                     /locus_tag="M2A35_004922"
     CDS             4228..5046
                     /locus_tag="M2A35_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCB7201101.1"
                     /translation="MQLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLLLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            5046..5291
                     /locus_tag="M2A35_004923"
     CDS             5046..5291
                     /locus_tag="M2A35_004923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201102.1"
                     /translation="MQHVSEGVIQVPELPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            5385..>5746
                     /locus_tag="M2A35_004924"
     CDS             5385..>5746
                     /locus_tag="M2A35_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCB7201103.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRIRFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDENWVLFNAMNGN
                     RAEMSPEAAGIAACLMTY"
BASE COUNT         1372 a         1512 c         1541 g         1321 t
ORIGIN      
        1 ctgggcggat acctgaatct gatacacaac gcctccggcc tgtgggctga cattgtggcc
       61 cagggaaccc gccacagcat gaaagcgtca tcggacaata acgacttccg cgcacggggc
      121 cggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caacctgatg
      181 ctggagccac aactgcagta tacctggcag ggactttccc tggacgacgg ccaggataac
      241 gccggttatg tgaagttcgg gcatgacagt gcacaacatg tgcgtgccgg tttccgtctg
      301 ggcagccaca acgatatgaa ctttggtaaa ggcacctcat cccgtgacac cctgcgcggc
      361 agtgccaaac acagtgtgcg tgaactgccg gtgaactggt gggtacagcc ttctgttatc
      421 cgcaccttca gctcccgggg ggacatgagc atgggtacag ccgcagccgg cagtaacatg
      481 acgttctcat cgtcacagaa tggtacgtca ctggacctgc aggccggact ggaagcccgt
      541 gtccgggaaa acatcaccct gggcgttcag gctggttatg tccacagcgt cagcggcagc
      601 agcgctgaag gctataacgg tcaggccacg ctgaatgtga ccttctgaca gaaccatcgc
      661 ctctctgtgg ccccggtcat catgatcggg acccggaccg gcgcaacgaa tattcaacgc
      721 cacattcgct ggcattaaca ataacatgat attcatcacg gagtgactat gttacagata
      781 gtcggcgcgc tgatcctgct gatcgcagga tttgccattc ttcgcctttt gttcagagca
      841 ttaatcagca cggcttctgc gctggcaggg ctcatattgc tgtgtctgtt cggcccggcc
      901 ttactggctg gctatatcac cgaacgcata accaggttgt tccatattcg ctggctggca
      961 ggcgtatttc tgacgattgc cggaatgatc atcagcttca tgtggggact tgatggtaaa
     1021 catatcgcgc tggaggctca cacctttgac tctgtgaaat ttattctgac caccgctctc
     1081 gccggtggtc tgctggctgc tcccctgcag atcaaaaaca ttcagcagaa cgggatcaca
     1141 ccagaagata tcagcaagga aattaacggg tattactgct gtttttatac tgcctttttc
     1201 cttatggcgt gttctgcatg cgcaccattg atcgcgttac agtacgatat ttcaccgtca
     1261 ctgatgtggt ggggcgggtt gttgtactgg ctggctgcat tagtgacgct gctatgggcg
     1321 gccagccaaa tccaggcgct gaaaaaactg acctgtgcca tcagccagac actggaagaa
     1381 caaccggtgc tcaacagtaa atcgtggctg accagtttgc aaaacgatta cagccttcct
     1441 gactcactga cggagcgcat ctggctgacg ctcatttctc aacggatttc ccggggagag
     1501 ctgagggaat ttgaactggc agacggaaac tggttactga acaatgcctg gtatgaaaga
     1561 aacatggcag ggtttaacga acagttgaaa gagaacctgt cattcacacc tgatgaactg
     1621 aaaacgctct tccggaaccg cctgaattta tcaccggaag cgaatgacga ttttctcgat
     1681 cgttacctgg acggcggtga atggtatccc ttttcagaag gtcgccgttt tgtatcattc
     1741 catcacgtgg atgagcttcg tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc
     1801 ccggaaaatc ataagccgga tccggaatgg tactgctcct ctctttgtcg cgaaacagaa
     1861 acactgtgtc aggaaattta tgaacgccct tacaacagct ttatttccga tgcaacggcg
     1921 aatggtctga ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct
     1981 tccggagggc agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc
     2041 cgtctgaaaa acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg
     2101 gtcagcggaa cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg
     2161 gcattcgacg gacaaaacgg acagtatcgt tacatgggaa ataatggccc catgcaactg
     2221 gaagtccccc gtgatcagta tgccggcgct gttgaaacca tgaggaataa gatccgcgaa
     2281 ggtaaagtac ccggtgtaac cgatcccgca gaagcgtccc ggctgattcg tcggggacat
     2341 ctgacttata ctcaggcccg taatatcacc cggttcggga ccatcgaatc ggtcacttat
     2401 gatattgccg agggttcggt tgtcagtctg gcggccggag ggatcagttt tgccctgacg
     2461 gcatcggtct tctggctcag caccggcgat cgcgatgctg ccctgcagac agctgctgtc
     2521 caggcaggaa aaaccttcac ccgcacactg gctgtctacg tcacaaccca gcaactccac
     2581 cgactcagtg ttgttcaggg tatgctgaag cacattgatt tttcgactgc cagcccgact
     2641 gtccgactgg ctcttcagaa ggggaccggt gcaggaaata tcagtgccct gaacaaagta
     2701 atgaagggta cgctggtgac atctctggca ctggtagctg tcacaaccgg ccctgacatg
     2761 atcaaaatgt tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca
     2821 tcttccggtg tggcaggtgg agctgtcggg tcagtggcgg gcgggatact gttcagtcca
     2881 ctgggaccat ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt
     2941 gcctccgctg tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt
     3001 ctggcaatga ttcaggagca ggtgacatag cttgccggca gtttcctgct gaccggacat
     3061 gagattgaaa atctgaacga gaatctggcc cgtgttatcg atcagaatgc tctggagatc
     3121 attttcgccg ccggtataca acaacgggcc gcgaccaata tgttaatcaa accactggtg
     3181 gtcagtatca tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaatatg
     3241 gttaaccgac tggaagaagc attacccccg gagttaccgg cataacggct attctgcgcc
     3301 ttttacgctt tatcacctga tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc
     3361 atgattcacc tgttcaaaac ctgcatgatt accgccttca ttctggggtt aacgtggtct
     3421 gccccactcc gggcacagga tcaacgttac atcagtatac gcaatacaga tacgatatgg
     3481 ctcccgggaa atatttgtgc ttaccagttc cggctggata atggcggaaa cgatgaagga
     3541 tttggccccc tcaccatcac tctgcaactc aaagacaaat atggtcagac gctggtgacc
     3601 agaaaaatgg aaacggaagc ttttggtgac agtaatgcca cgcgaaccac agacgcattt
     3661 ctggaaacgg agtgcgtgga aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa
     3721 agtaacggcc atcgtgtcag tctgccgtta tcggttttca atccccaaga ctaccatcca
     3781 ctgctgatta ccgtttccgg aaaaaacgtt aactgatccc tgaagtcagt ttcatgcttc
     3841 agcctctgtc acaacgcgtg gcagaggctt tttatttatc aggagaaaac agatatggcg
     3901 ggttacacag agtatgtcct ggctgaagga tctttctcat acgggcaggc ggttgctgtc
     3961 ataaccgctt accgcaatgt gtttatccag gatgacccgg gaatgcattt ccgtcgggtt
     4021 atccgcaacg cggaaggtca gcgacgctgg cgctgccgga attctgaacc cgatgccgga
     4081 aaggtgctta atacgcggct cgccagtgac ggtcttctca gacaataaac gtcattcatt
     4141 tcatctgtca ggccgcgtct tctccgggag acgtggcttt ttcatttatt acaccagtaa
     4201 atcttaacca ccgataagga gcaaattatg caactagcga gtcgttttgg ttatacgaac
     4261 cagatacgcc gtgaccgtcc gctgacacac gaagaactga tgcactatgt tcccagtatt
     4321 ttcggtgaag accggcatac ttcccgcagt aaacgatatg cgtacattcc caccatcacc
     4381 gtactggaaa gcctgcagca ggaaggcttt cagccattct tcgcctgcca gacccgtgtg
     4441 cgcgacccgg gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag
     4501 ataaacggag aacatgtccc tgaaattatt ctgctcaact ctcatgacgg tacctccagc
     4561 taccagatgc tgccgggtta cttcaggttc gtctgccaga acggatgcgt ctgtggtcag
     4621 tctctggggg aagtgcgtgt tccgcaccgg ggaaatgtgg tggacagagt cattgaaggg
     4681 gcttacgagg tggtgggcgt gtttgaccgg atagaggaga agcgtgatgc catgcagtcg
     4741 ctgctcttac cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat
     4801 ggtgatgaac atcagcccgt caccactgcc gacattctga caccacgacg ccgggaggat
     4861 tacggtcagg acctgtggag tgcttatcag accattcagg agaatatgct gaaaggcgga
     4921 atttccggtc gcagtgccag aggaaaacgt atccacaccc gtgccattca cagcatcgac
     4981 accgatatta agctcaaccg cgcattgtgg gtaatggcag aaacgctgct ggagagcctg
     5041 cgctgatgca gcatgtctct gaaggcgtga ttcaggtacc agaattaccc gacgccccgg
     5101 aggcgctgcc ggggtgtcgt tccatttctg atgatgacct gtgcgcctgg tgtacccgtc
     5161 tgctttaccg tcccggagaa ctcagtctgt gcagactgag tacaggcaat ggcatctggc
     5221 catccgtctg tgaccggaac ggttacgcgt acggctgtcc ggaatttcag ccgaatatca
     5281 ttcatccctg atattaccgg gtctcacggt cagtgatgaa cgtgatgttg ccagctcccg
     5341 acctttactc cctctcattt attcacatta caaggatttc atacatgaaa acattgtctc
     5401 agaacaccac ctcttcggcc tgtgcaccgg agactggcct gcaacaactg gttgccacca
     5461 tagtccctga tgaacagcgc atcagattct ggccgcagca ctttggcctc attccacagt
     5521 gggtgaccct ggagccccgt atcttcggct ggatggaccg tctttgcgaa gactactgcg
     5581 gtggtatctg gaatctgtac acactgaaca acggtggcgc atttatggcg cccgaaccgg
     5641 atgacgatga tgacgaaaac tgggtactgt tcaatgccat gaacggtaac cgcgctgaaa
     5701 tgagcccgga agccgccggc attgccgcct gtctgatgac gtacag
//
LOCUS       DAHBHC010000168         5735 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHBHC010000168 DAHBHC010000000
VERSION     DAHBHC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.172"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(126..425)
                     /locus_tag="M2A35_004925"
     CDS             complement(126..425)
                     /locus_tag="M2A35_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201104.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFAATFWARKLDCIHNQFPLE"
     gene            complement(769..1050)
                     /locus_tag="M2A35_004926"
     CDS             complement(769..1050)
                     /locus_tag="M2A35_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7201105.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENLHAARRLKIRIETCVLALS
                     EHPYLYPSSDRVSGLREIVAHPNYIILYRVATSSIEIVSVAHARRQFPR"
     gene            complement(1050..1241)
                     /locus_tag="M2A35_004927"
     CDS             complement(1050..1241)
                     /locus_tag="M2A35_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCB7201106.1"
                     /translation="MNTALSPIVSEFETIEQENSYNEWLRTKVAASLADPRPAIPHDE
                     AMAEIENLIAQISATNGSV"
     gene            complement(1272..1670)
                     /locus_tag="M2A35_004928"
     CDS             complement(1272..1670)
                     /locus_tag="M2A35_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201107.1"
                     /translation="MTTLEITTYRIKLVLEKTGLKQAELARRIGVAQQSVQKWVHGIT
                     SPSTANLDKLSDVTGFPPYWFMLPPSEEEQVVVPDTMKIGPRQRELLQTFGAFPEEDQ
                     EQMLKDMKDKKESMDRTVARWLAAQKGHRA"
     gene            1787..2053
                     /locus_tag="M2A35_004929"
     CDS             1787..2053
                     /locus_tag="M2A35_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201108.1"
                     /translation="MHECLKKKIHKRGLSQAGVARLIGLPQQVVSRWANGHQVPASRV
                     LQLCEIMQWEVTPHELRPDIYPNPTDGLPSEFKANTQPAAGVDS"
     gene            2050..2475
                     /locus_tag="M2A35_004930"
     CDS             2050..2475
                     /locus_tag="M2A35_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7201109.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELHD
                     DSKHNALYLNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNSVAVH"
     gene            2547..3617
                     /locus_tag="M2A35_004931"
                     /pseudo
     CDS             2547..3617
                     /locus_tag="M2A35_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
     gene            3658..4080
                     /locus_tag="M2A35_004932"
     CDS             3658..4080
                     /locus_tag="M2A35_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCB7201110.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLESKTGATRYLIS
                     VLARELVASGDIYNSGYGLFPSEQARKDWQNARKKLSRENLKKPSMVDPDLIWSLPDG
                     EIRRYDRRQNIICSECRKSEVMQRVLAFYRGNFQEVLL"
     gene            4229..4411
                     /locus_tag="M2A35_004933"
     CDS             4229..4411
                     /locus_tag="M2A35_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201111.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            4404..4580
                     /locus_tag="M2A35_004934"
     CDS             4404..4580
                     /locus_tag="M2A35_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201112.1"
                     /translation="MADFGSTKYNVSFKEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            4577..5134
                     /locus_tag="M2A35_004935"
     CDS             4577..5134
                     /locus_tag="M2A35_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCB7201113.1"
                     /translation="MSGINYQMLRDKAENATKGGYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAEASPATVLALLDEREAANKRIAELEARE
                     IKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKAIGVPVKWTNGDAEKRMTGADWLTAI
                     EMYKPTVRIVEVNVPRAAGIRIKGE"
     gene            5136..5345
                     /locus_tag="M2A35_004936"
     CDS             5136..5345
                     /locus_tag="M2A35_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201114.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFSPGHKVLRINK"
     gene            5342..>5735
                     /locus_tag="M2A35_004937"
     CDS             5342..>5735
                     /locus_tag="M2A35_004937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201115.1"
                     /translation="MTTFTDDDKKLIKEIKERISSLEVRDDIERRAYEIALASLEAEP
                     VAWLHSDNGLGIPAITRSKNIADSWLSKSWYVQPLYIAKPVPVVPDARPSLNNGIVGF
                     DEGWNACRAAMIHAGNFRENSNSSTNNFR"
BASE COUNT         1596 a         1240 c         1525 g         1374 t
ORIGIN      
        1 aagttcatca tcggtatagt ctggcgcgat gatgacgggt tctcgtttct gcatgtcggc
       61 tccttgtggt tagcgttgcc tgcttttaac cacgtcaggc gaggtggtat cctctgaggg
      121 atctgttact cgagaggaaa ttggttatga atacaatcaa gttttcttgc ccagaatgtg
      181 gcggcgaagt ctttgacaca tcctttaaac cgcatggctc tgacagtttc gcgggagcca
      241 tctgcaaaaa ttgtggtcac cttgtaactg aagatgagtc ctcgcagttc gatgacgaaa
      301 tcgttgacaa tatcttcggt gcactcacca gagactttct gaagtaaagg cgcataccgc
      361 ttagttaccg ctctgataac tcttacctgt ccggcaatgg cgctgatgtc aatataaagc
      421 gccattgccg cttctttgcc gatcccggga tgccttccat tctgatgttt gacttcgccc
      481 actgagaaac cctctgtttc cccttcacgc cggggtagcg gaacaaaaac ctgctgcata
      541 gttaaactta aaccctgccg tcactttcga atgcctcggg ctggctacat tgacccctga
      601 ccacctgcct ggtaactcga agtattgccc gacattctgt ggggcggggt gggttggtga
      661 aataagaatg cacttttaaa gttatgatgt caacacttat aaagttagat tcatgcaata
      721 aaaaaccgcc tccatgtgaa gcagttttgt gaaaaaagag gaaggggatt acctaggaaa
      781 ttggcgtctg gcgtgggcga cgctcacaat ttcaatactt gaagtggcca cacggtacag
      841 aatgatatag ttagggtgag ctacaatctc gcgcaaccca gacactctgt cgcttgatgg
      901 gtataagtac ggatgttcgg ataaagctaa aacacatgtc tcaatgcgaa tttttagtct
      961 acgtgcggca tggagatttt cttttgcaat ataggctacg atctgacgta aatcatcacg
     1021 cgcagaaggt agccataaga tgggcagcat cacacactcc cgttagtcgc agaaatttgg
     1081 gcaatgagat tttctatttc tgccattgcc tcatcatggg gaattgcagg gcgcggatct
     1141 gcaagacttg cagctacttt tgtacgcagc cattcgttgt agctgttttc ttgctcaatg
     1201 gtttcaaatt cagaaactat aggcgaaaga gcagtgttca taacatatct ccgtttggct
     1261 tatgatggcg actacgctcg gtgccctttc tgggccgcga gccatcttgc aacagtgcga
     1321 tccattgact cttttttgtc tttcatgtct ttaagcattt gttcctgatc ttcctctgga
     1381 aatgctccga atgtttgaag cagttccctt tgcctggggc ctattttcat ggtatctggc
     1441 accacaactt gctcttcttc gcttggcggt aacataaacc aatacggggg aaagcccgtt
     1501 acgtctgaaa gtttatcaag attagcagtc gatggacttg taatgccatg aacccacttt
     1561 tggactgact gctgggcaac acctatcctg cgggctagct ctgcctgctt aagtcctgtt
     1621 ttttccagca ccaattttat tcggtacgta gttatttcaa gggtggtcat tcgcttcatg
     1681 cattcatttt acataaaaaa agtgtaacta gcacaatcag cttaaaagtg ttgataatat
     1741 aactttaaaa gtgtataaat catggcaatt cacgggagtt aacaatatgc atgaatgttt
     1801 aaaaaagaaa attcacaaaa gagggttatc ccaagcaggc gttgctcgct tgattggatt
     1861 gccgcaacag gttgtatctc gatgggctaa tggtcatcag gttcctgcgt caagggttct
     1921 acaactgtgt gagattatgc agtgggaggt tactcctcac gaactccgcc ctgatattta
     1981 ccccaatcca accgacggct tgccttctga gtttaaggct aacacacaac cagctgcggg
     2041 agttgattca tgaaaatcaa gcatgaacac atccgcatgg cgatgaatgc ctgggcgcat
     2101 ccggacggcg aaaaagtacc ggctgcgaaa attaccaaag cgtatttcga gctgggtatg
     2161 acgttccctg aactgcatga cgacagcaaa cacaacgcgc tttatctcaa tacccagaaa
     2221 attttccgct gggtagagaa agacacccct gatgcagttg aaaaaattca ggcgttgtta
     2281 ccagcgatcg aaaaggcaat gccacctttg ctggtggcca gaatgcgcag ccacagttca
     2341 gcttattttc gggagctggt ggagacgcgg gagcgactgg tgagagacgc tgatgatttt
     2401 gtcgcagtgg caatcgccgg tttcaatcag atgaaccgtg gtggcccggc aggaaattct
     2461 gtggcagtac attgactgac aatagccata tcgaatcgct tccggcaact cgtgagtaaa
     2521 aagattcggt atcagaagag gtgagtatgg ctaacgcctg gctcagatta tggcatgaca
     2581 tgccaaacga tcctaagtgg cgaacaattt ccagggtgtc aggacagcca atcgcaacag
     2641 tgatggctgt gtatatccac ctcctggtga gcgcgtcacg aaatgttacg cgaggtcaca
     2701 ttgatgtcac gacagaagat ttggcaagtg cgctcgacgt gacagaagag gtaattgatt
     2761 caattttgca gacgatgcag gggcgggtac ttgatggtga tttaatcact ggatgggaaa
     2821 aacgccaggt gcttaaagag gacaacggca atatttcgca aaccgcaaaa tctcctgcag
     2881 agcgcaagag ggcgcagcga gagagggaaa gaaagcggga acaaaatggc gattgtcacg
     2941 gcgcgtcacg aaatgtcacg cacatgtcac gacgagtcac gacagataca gatacagata
     3001 aagatacaga tcaagaagat caaaacacta tggtccatgg cgtaaaaaac gccatgaacc
     3061 aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca ccggaagccg
     3121 attcagcgta tgcgctgaaa gccgattcgg gcgctgtgca gcaggtgatg accgcaaggc
     3181 cggagcaatc gcacccactg cagcagcctg aagccgattc cgccatttag cgggaagccg
     3241 atcgggtagt cccggaaaaa aacgggcagt ctgtgggaag agtggattat ccggatgtgt
     3301 tcgaacaggt ctggcgggag tacccgttgc gtgccggggc aaacccgaag aaatccgctt
     3361 tcagtgcctg gaaggccaga ttacgcgagg gggtgccacc agaggccatg ctggatggcg
     3421 tgaggcgtta cgcaagatac ctggcggcta ccgggaaaac gggaacggaa tttgttcagc
     3481 gagcgacgac gtttttggga ccggaccgga attttgagaa cccctggttg ctcccggtaa
     3541 gcggtacgaa caaccagcgt tgtgtgaatc atatttctga accggatacc gaaattccac
     3601 cgggcttcag ggggtaagtg tgtatttctg gtcatgaggt aattttcagg agggcttgtg
     3661 gcaaaagttt ttacacaaga agagcgagaa aaaattaaag ggcaggttgt tgagctagta
     3721 cgccggagtg ggcgcgagac gctacggcaa ctggaatcca agacaggtgc gacaagatat
     3781 ctgatcagcg ttctcgccag agagctggtt gccagtggcg atatatacaa ctctggttac
     3841 gggttattcc cgtctgaaca ggctcgtaag gactggcaaa acgcccgcaa aaaactctca
     3901 agggaaaatc tgaagaaacc atctatggtt gacccggacc tgatctggtc attaccagac
     3961 ggagaaatac gccgctacga caggcgtcag aacataatct gtagcgagtg ccggaagagc
     4021 gaagttatgc agcgcgtgct ggctttttat cggggaaact ttcaggaggt gctgttgtga
     4081 gccaaattaa caatcggaac ttggtgaaga gaaagcataa tccaaatctg aataattaaa
     4141 ttgagcactg ttaaaaattt aatccttaac cggagggatt tctgcaccct cagaacagca
     4201 ggaggccacc agaaagggcg gtagtgaaat gcgaaagttc aaaataatta ttgaaactgg
     4261 aatagctggt ggggattttg aggatgtatt cgaagtggat gatgacgcaa cacctgatga
     4321 aattcatgac gaagcaaaag aaattttctt taactactgc aattactcat atcacgaaat
     4381 aaaagacgag gaggaagaac aaaatggctg attttggttc aactaaatac aacgtcagtt
     4441 ttaaagaatg gcatgaactg ttaatggact atgcagagtt acgcggtgga agtgctgctg
     4501 atgctgaagc atggcgtgat gattatgaag cagggaaaac tccggtcgaa gcatattgtg
     4561 atgagtgggg cgatgaatga gcgggattaa ctatcagatg ctgcgggata aggcagagaa
     4621 cgcaactaaa ggaggctaca tcgtagggca tacatctgtt aaccagcacg gtaatttaac
     4681 aggagttttt gtttgtcaaa aatggaaagg agaaccaggt ggcgtgattg cagaatgtca
     4741 tgttaactgc ctggttgaaa cagatgctca ggcttatgca aacgcagaat tcatagcaga
     4801 ggctagcccg gctaccgtgc tggcactact ggatgaacgg gaagcagcca acaagcgcat
     4861 agcagaactg gaagcacggg aaataaaacc agccaaaggc gaagttcttg tcgttgtatc
     4921 tggttttact ggttgcggaa aaagcgccat cgccggggaa atagaaatcg cgatgaaggc
     4981 tattggtgta ccggttaagt ggactaatgg cgatgcagaa aagcgcatga ctggcgctga
     5041 ctggctgaca gcgattgaga tgtacaaacc aactgtgcgc atcgtggaag ttaatgtgcc
     5101 acgcgccgct ggcattcgca tcaaaggaga gtgatatggc tatcgctgca agttacacca
     5161 tgcatctcta ttgtgattgc ctccagtgta cagatggcaa atataagtcg ccagacttcg
     5221 gtgagtatat aggtacgtca tgggctggct gtgcaaaaga ggcgcgcaag gatggctggc
     5281 gaataagcaa agacaaaacg cgtgcttttt cacccgggca taaagttttg aggattaaca
     5341 aatgacaaca tttaccgacg acgacaagaa actaattaaa gaaatcaaag agcgtatcag
     5401 cagcctggag gttcgagacg atattgagcg ccgggcttat gaaatagcgt tagcctcgct
     5461 ggaagcagag ccggtggcat ggctgcattc agacaatggc ttaggtattc cggcaataac
     5521 gaggagtaaa aacattgctg acagttggtt atcaaagagc tggtatgttc agccgctata
     5581 tatagccaag ccagtgccgg tggtgccaga tgctcgtccg tctttaaata atggcatagt
     5641 cggctttgat gaaggctgga acgcttgccg cgccgccatg attcatgccg gaaactttcg
     5701 ggaaaactcg aattcgtcaa ccaataattt tcggg
//
LOCUS       DAHBHC010000169         5690 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHBHC010000169 DAHBHC010000000
VERSION     DAHBHC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.173"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1348
                     /gene="paoC"
                     /locus_tag="M2A35_004938"
     CDS             <1..1348
                     /gene="paoC"
                     /locus_tag="M2A35_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HCB7201116.1"
                     /translation="TLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYA
                     GANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQ
                     VDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLL
                     EKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPV
                     SAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEA
                     TAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILN
                     PKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLD
                     DTDPISSPMKAKGVGELGLCSVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1358..2314
                     /gene="paoD"
                     /locus_tag="M2A35_004939"
     CDS             1358..2314
                     /gene="paoD"
                     /locus_tag="M2A35_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HCB7201117.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            2365..2703
                     /locus_tag="M2A35_004940"
                     /pseudo
     CDS             2365..2703
                     /locus_tag="M2A35_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2992..4269
                     /locus_tag="M2A35_004941"
     CDS             2992..4269
                     /locus_tag="M2A35_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7201118.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRVQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMMALCLIGAYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARLVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKKYIELLRYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(4351..5064)
                     /locus_tag="M2A35_004942"
     CDS             complement(4351..5064)
                     /locus_tag="M2A35_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201119.1"
                     /translation="MATAGILTIRSANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVALKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(5096..>5690)
                     /locus_tag="M2A35_004943"
     CDS             complement(5096..>5690)
                     /locus_tag="M2A35_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201120.1"
                     /translation="PKVTEEFLKNLSNLTPYFTKLGEATGSLFEAIMKIVNWFNRNDD
                     KTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTTPDNSHSLFNTIKGLLFD
                     DNSSVSDVKMSVNEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVVTEVPLTIN
                     SDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
BASE COUNT         1455 a         1363 c         1431 g         1441 t
ORIGIN      
        1 cacccttcag cacttgcgta tcggtgccga ccagagcggg aaaatcaccg ctatctcaca
       61 tgaaagctgg tctggaaacc tgcccggcgg cacgccggaa acggcggtac agcaaagcga
      121 attactctac gccggggcga atcgtcatac cggcctgcgg ctcgccacgc ttgatttgcc
      181 ggaagggaac gccatgcgtg cgcccggcga agcccccggt ctgatggcgc tcgaaatcgc
      241 gatcgacgaa ctggcggaaa aagcgggcat cgatcccgtc gagtttcgca tcctgaatga
      301 cactcaggtt gaccccgccg acccgacgcg ctgcttctct cgccgtcagc ttatcgagtg
      361 cttgcgcacc ggagcggata aatttggctg gaagcagcgc aacgccacac ccggacaggt
      421 gcgcgacggg gagtggctag tcggccacgg tgttgcggcg ggctttcgca ataatctgct
      481 ggaaaaatcg ggtgctcggg ttcacctcga acaaaacggc accgttaccg tagaaacgga
      541 catgaccgac attggcaccg gcagctacac cattctggcc cagacggcag cggaaatgct
      601 tggcgtaccg ctggagcagg ttgcggttca cctcggcgat tccagtttcc cggtttctgc
      661 gggttctggt ggacaatggg gcgcgaatac ctccacctcc ggcgtttacg ccgcctgtat
      721 gaagcttcgc gaaatgattg cctcggcagt cgggtttgat cctgagcagt cgcagtttgc
      781 cgacggcaag attaccaacg gtacccgaag cgccacgcta catgaagcca ccgcaggcgg
      841 cagactgaca gcggaagaga gcattgaatt cggaacactg agcaaagagt accagcagtc
      901 gacctttgcc gggcattttg tggaggtcgg cgtgcatagc gcgacgggag aagttcgggt
      961 ccggcgtatg ctcgctgtgt gtgctgcagg acgcatcctg aatccgaaaa ctgcgcgcag
     1021 ccaggtcatt ggcgcaatga ctatgggcat gggcgcggca ctgatggagg agctggcggt
     1081 ggatgaccgt ttgggctact tcgttaatca cgatatggcg gggtatgagg tgccggtcca
     1141 tgcggatatc ccaaaacagg aggtgatttt cctggatgat accgacccca tatcctcccc
     1201 gatgaaggcc aaaggtgtcg gtgagctggg cctgtgcagc gtgagcgcgg ctatcgccaa
     1261 cgcggtgtat aacgccaccg gtattcgggt acgggattat cccatcactc tggataagct
     1321 gctcgataag ctgccggatg tggtttaagg agaaacaatg tcatacccgc tttttgataa
     1381 agacgaacac tggcataagc cagagcaggc gtttctcacc gatgaccacc ggaccattct
     1441 gcgcttcgcc gtagaggcgc taatgtccgg taaaggagcg gtgctggtga cgctggtgga
     1501 gatacgcggc ggcgcagcgc gcccgctcgg ggcgcagatg gtggttcgcg aagatggtcg
     1561 ttactgcggt tttgtctctg gcggctgcgt ggaggccgct gccgcttttg aagcgctgga
     1621 gatgatgggc tcaggccgcg atcgcgaaat tcgctatggc gaaggttcgc cgtggtttga
     1681 catcgttctg ccctgcggcg gtgggatcac gctgacgctc cataaactac gctcggcaca
     1741 gcctctgctc gccgtgctga accgtctgga acagagaaaa ccggcggggc tgcgctacga
     1801 tccgcaagca caatcgctgg tgtgcctgcc cacgcaaacc cgaacgggct ggaatctcaa
     1861 tggctttgag gtggggttca ggccatgcgt caggctgatg atttacggac gttctcttga
     1921 ggcgcaggca actgcgagtc ttgcagcagc cacaggctat gacagccata tcttcgatct
     1981 ttttccggcc tcagccagcg ctcagatcga taccgatacg gcggtcattt tgctgtgcca
     2041 tgatctcaac cgggagctgc cagtgttgca ggccgcgcga gaagcaaaac ccttttatct
     2101 cggcgcattg ggcagctatc gaacccacac gttacgtctg caaaagctcc acgagctggg
     2161 atggtccagg gaggaaacaa cgcaaatccg ggcacccgtc gggatattcc ccaaagcccg
     2221 ggatgcgcat actctggcac tctccgtgct ggcagaagtc gcctctgtac gtctccatca
     2281 ggaggaggat tcatgcctgc ccccgtcgtc ctgatccttg cggccgggcg tggagagcgc
     2341 tttctcgcct ccgggggaaa tacccataag tgtatcggct ggcgtcagtc cccggaggtt
     2401 gcgccttatc gctggccatt tgaagaaaac gggagaactt tcgaccttgc gattgaaccg
     2461 cagattacga ctaatgatct gcgtctgatg ttgaggctgg ctcttgccgg cggaggaata
     2521 acaattgcca ctcaggaaac tttcaggcca tatattgaaa gcggtaagct tgtatcgctg
     2581 cttgatgact ttcttccaca atttccgggc ttctatctgt atttcccaca gcgtcgcaat
     2641 attgcaccaa agctccgcgc cctgattgac tacgtcaaag aatggcggca gcaattggtt
     2701 taaatagctg cacctgcact gcctgatgtc agaacagtat tttgatgaat tgccaaggtt
     2761 acaatggcac aaatgcggca caggaggaaa cgtggtgtat ttaaatatgg ggtaactctt
     2821 tgattttaat ggtgccgata ataggagtaa aacactcgat taatctcgtg ttaatgccat
     2881 cacctacgac cattactaat tgattgaatc ttatagaaaa aattatcatt ttctatgcgt
     2941 tacaatcaat tttcacatta caaacgctcc gacactttag ccggggagtt tatgagatac
     3001 aagcccaatc aatacataat ctgtgattct tacggtaact actatttgag gatcacgttg
     3061 cctgtgtata tgcagccctt ttttgaagga aaaaggacgt ttgtcagaag tctacacaca
     3121 agtaatcttc gtgttgcacg tagaaagcgt gatcagattg cagatgaata ccattgttta
     3181 cgggagagtg tcgctcctgt aaacagcaca atagagaaca cgctggaact gttacgcagt
     3241 aaggctaaat acgccaaaac agctaccaga gtgcaagata cagcgtcttc gtgtccgtca
     3301 ttgcttaaaa ttcttgaaat ctacctgaca attaacagca cgaagaagaa gccagccact
     3361 ttagctaagg caagaaaagc ggtagagatg tttctctcct accgtaaaaa gcctgatatt
     3421 gcattgcaag atgtgagccg caccactgtt acaggctgga ttgaacacat gcaaaaaacc
     3481 ctttcacaac aatcaattgc aaattatatc agcccaatgg cccagctatg ggagttagct
     3541 tcatcacgtt accacgatgc gccagaaagg gcgctctccc cctggcgagg gcataggctt
     3601 gatgtggcac aaagtagaga gagctatgag gcattttcta acaaagagct attgcaagtg
     3661 ttgcaagtat tttccggtaa ttcagcagaa aacaaagaaa tgatggcttt gtgtcttatc
     3721 ggtgcttata caggtatgcg gatcaatgag atagcaagtc tcacaataga cgatgtgaaa
     3781 gagatcgaag gtgtgctgtg ttttgaaatc acacagggaa aaacgaaagc tgcggcacgt
     3841 cttgtgcctg tgcatagcct tatcactcca ttggtgttat ctcttcgtga aaagcctcac
     3901 aatggctttt tgttctatca cgccagcatt acagagcgtg ctgatggtaa acgttccacg
     3961 tggcacacgc agagatttac aagagctaaa cgaaaggctt taggggaaaa gggaacagaa
     4021 aggaaggtgt ttcattctct gagacacgga gtagcacagc ttcttgatcg aaatcaaatt
     4081 ccagaagaca ggatcgccct tctcctgggc catacacgcg gtaatacaga gacattccgc
     4141 acatatagca agaatgcagc ttctccagta gagcttaaaa aatatattga gcttctacgc
     4201 taccctgaaa tagagaaagg cttatcaatc aataaaaaat caaatttaag gcgtaaaaca
     4261 acgccataga cgcaataaag aggcttcaac atagattggt agcctctttt ataattacat
     4321 cgttacagag cgttttagag gcatttacag ctattcctgt ttaaaggcag gagtaatagt
     4381 gtcttcacct tcactacttg atttttcagt gttaacacgt tttttcatat tagccccaat
     4441 gttcttagat ggctgtgttt tgagagctac agtgtttaca gtgccatacg aaacgagcct
     4501 gatctgctca aagctaagat caaataccag ggattcgctt gcctcttctg tgtattcgat
     4561 gcctgttaaa acaacgttct caaaagtttt attttctgta acaagaagaa aaggctgtct
     4621 actgtccata atctgattca ggtagtcgat agctaatgat cttctgttcc cgttcacccc
     4681 tgcactcttt aacaagccag gatcgctttt aatcggtgtt tcagtgatcc ttcctttaag
     4741 agaaaattta ttgtttttaa tttgaacgtg atcagacaca gtagcaccat tttctacggg
     4801 gtagcttgta acatctgccg aacgtctcac gttcacgtcg tcaacactgt caaaggccag
     4861 tgccatataa ttttcataaa cgtcattgcc agaagatgcc agtccactgg taacaatagc
     4921 aaatccgttc tctccttttg ttttattagc tttgctattt tcgttttttg tagagtttat
     4981 atcttgttct gctttgtaaa cagcctgaac gtgttgttct ggagtatttg ctgatcgtat
     5041 ggttaaaatt cccgccgttg ccataataat tttccttgta atgttatggc gggaattacc
     5101 cgccgttgga ttgtcctgat aagctggtta atgttaatga tttcaagaag ctatcccgac
     5161 ttgctctcgc tgtaaaatcc acataatcgc ttaatcttga tgtgtcagaa ttaatagtaa
     5221 gaggaacctc tgttaccact tcaaaggtcg gattcagatt aaacacagga gataaattat
     5281 aagccggagt tgtcattgca agattattta aagcaccctg tttgagattg gttatcggtg
     5341 cttcattgac tgacattttt acatctgaca cagaagaatt atcatcgaaa agcaatccct
     5401 taatcgtgtt aaagagagaa tgactattat caggtgtagt tgttgcagtg ccagcgcctt
     5461 tatattggtc aggaacgaga ttagaaagat gctgtttcat cccttcatta ctcaaaggcg
     5521 gtgcatcacc taaatttttc tgaatagcat ctgtcttatc atcattacga ttaaaccagt
     5581 ttacaatctt cataatagct tcaaagagac ttcctgtagc ttctcccagc ttcgtaaaat
     5641 acggagtgag attagaaagg tttttcaaaa actcttccgt tacttttgga
//
LOCUS       DAHBHC010000170         5529 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHBHC010000170 DAHBHC010000000
VERSION     DAHBHC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.174"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..726
                     /locus_tag="M2A35_004944"
     CDS             <1..726
                     /locus_tag="M2A35_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7201121.1"
                     /translation="EYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVRIPHLFPG
                     DDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWR
                     YFMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRM
                     AIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWI
                     AQAYQQQIAESRRPEEETTPRET"
     gene            716..2221
                     /locus_tag="M2A35_004945"
     CDS             716..2221
                     /locus_tag="M2A35_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCB7201122.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            2258..2605
                     /locus_tag="M2A35_004946"
     CDS             2258..2605
                     /locus_tag="M2A35_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCB7201123.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGSALSHAALP"
     gene            2663..3691
                     /locus_tag="M2A35_004947"
     CDS             2663..3691
                     /locus_tag="M2A35_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCB7201124.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            3743..4126
                     /locus_tag="M2A35_004948"
     CDS             3743..4126
                     /locus_tag="M2A35_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCB7201125.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            4119..4472
                     /locus_tag="M2A35_004949"
     CDS             4119..4472
                     /locus_tag="M2A35_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201126.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            4487..5020
                     /locus_tag="M2A35_004950"
     CDS             4487..5020
                     /locus_tag="M2A35_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201127.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            5017..5412
                     /locus_tag="M2A35_004951"
     CDS             5017..5412
                     /locus_tag="M2A35_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201128.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            5420..>5529
                     /locus_tag="M2A35_004952"
     CDS             5420..>5529
                     /locus_tag="M2A35_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201129.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDW"
BASE COUNT         1230 a         1347 c         1762 g         1190 t
ORIGIN      
        1 gaatatatcg ccggtgcgcc gcaggggcag aaggataatc cgcttattaa tattctggag
       61 aagttctcca gctggtatga cacgaataac gtgacgctgg gcggtgtcag aattccgcac
      121 cttttccccg gggatgatct gaaactgcag actgcgcagg attcagacaa tggattttcg
      181 gcgcttgaac aggcgctgct gcggtatatc gccgccggtc ttggcgtttc ctacgaacag
      241 ttgtcccgtg attactcgaa ggtcagttat tcaagtgccc gcgcctccgc caatgagtcg
      301 tggcgctatt ttatggggcg gcgaaaattt attgcggccc ggctggccac gcagatgttt
      361 tcctgctggc tggaagaggc acttcttcgg gggattattc gtccgccacg ggcacgtttt
      421 gatttttatc aggcgcgatc agcctggtca cgggcagagt ggattggtgc cggaagaatg
      481 gccattgacg ggctcaagga ggttcaggaa tcggtgatgc gcattgaggc cggactgagc
      541 acgtatgaga aagagctggc gctgatgggc gaggattatc aggacatttt ccgccagcag
      601 gtcagggaat ctgcagagcg gcaaaaagcc ggactctcac gtccggtgtg gatagcgcag
      661 gcgtatcagc agcagatagc ggagagtcgc aggccggaag aggagacaac accccgtgag
      721 acgtaatctt tcacacatta ttgccgcagc attcaatgaa ccgctgcttc tggagcccgc
      781 ctatgcgcgg gttttctttt gcgcgctcgg gcgcgagatg ggggcagcaa gtctttcggt
      841 accacagcag caggtacagc ttgatgctcc cggaatgctg gctgaaacgg acgagtacat
      901 ggccggaggt aaacgaccgg cccgtgttta ccgggtggtg aacggtattg ctgtactgcc
      961 ggtgaccggc acgctggtgc accggctggg gggtatgcgg ccattttccg gaatgacagg
     1021 ctatgacggc attgtcgcct gtcttcagca ggcaatggcg gatagccagg tgcggggcgt
     1081 actgctggac attgacagtc cgggcgggca ggccgccggc gcgtttgact gcgctgacat
     1141 gatttaccgc ctccgtcagc agaagccggt ctgggcactg tgcaatgaca cggcctgttc
     1201 tgcagccatg ctgctggcgt cggcctgctc ccgacggctg gttacccaga catcccgtat
     1261 cggctccatt ggcgtgatga tgagccatgt cagctatgcc ggtcatctgg cgcaggccgg
     1321 tgtggatatc acgctgattt actcaggggc gcacaaggtg gatggcaatc agtttgaagc
     1381 cttaccggca gaggttcgcc aggacatgca gcagcgcatt gatgcggcgc gccggatgtt
     1441 tgccgaaaaa gtggccatgt ttaccggtct gtctgttgat gccgtcacgg gaacagaggc
     1501 cgccgttttt gaaggtcagt ccggcattga tgccgggctg gcggatgaat tagtcaatgc
     1561 gtcggatgcc atcagtgtga tggccacggc gctgaacagt aatgtcagag gaggcactat
     1621 gccgcaatta actgcaacgg aagccgccgc gcaggagaac cagcgagtga tggggatcct
     1681 gacatgccag gaagcgaaag gacgtgaaca gcttgccacg atgctggcag gacaacaggg
     1741 catgagcgtt gaacaggccc gggcgattct ggccgcggcg gcaccgcagc agccggtggc
     1801 atccacgcag agtgaagccg atcgcattat ggcgtgtgaa gaagcgaacg gtcgtgaaca
     1861 actggcggca acgctggcgg cgatgccgga gatgacggtg gaaaaagccc gcccgatcct
     1921 ggctgcttca ccgcaggcgg atgccggacc ctcactccgt gatcagatca tggcactgga
     1981 tgaggcaaaa ggggctgagg cgcaggctga acagctggct gcctgcccgg gaatgactgt
     2041 ggagagcgcc cgggctgtgc tggctgcggg atcaggtaag gcagaaccgg tctctgcatc
     2101 cacaaccgcc ctgtttgaac gcatcatggc gaaccattca ccggcagcgg tacagggtgg
     2161 cgtgccacag acgtcagcag acggtgatgc ggacgtgaaa atgctcatgg ctatgccatg
     2221 aagtcagtgc tgaccatcaa caggaggttt ttacaatatg gtaacgaaaa ccatcactga
     2281 acagcgtgcg gaagtacgta tttttgccgg taatgatccg gctcataccg ccacaggcag
     2341 cagcgggatt tcctcggcaa caccggcact gacgcccctg atgctggatg aagctaccgg
     2401 gaaactggtg gtctgggacg gacagaaagc cggtagtgcg gttggcatac tggtactgcc
     2461 gcttgaaggc acagagacgg tactgacgta ttacaagtcg gggacctttg cgacggaggc
     2521 aatccgctgg cctgacagtg tggatgaaca caaaaaggca aatgcctttg ccggcagtgc
     2581 cctgagtcac gcggcgctgc cgtaacacgt tatcaggcca ccgcggtggc ctgactgatt
     2641 tctgaatgaa aggaactgat ttatgggatt gtttacgacc cgccagttac tcggttatac
     2701 cgaacaaaaa gtgaaatttc gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa
     2761 tttccatacc gaagaggtga tgctggacaa aattaccgga aaaacgccgg tggcggccta
     2821 tgtctccccg gttgttgaag gaaaagtgct gcgtcatcgc ggtggtgaaa cccgcgtgtt
     2881 acgtccgggc tacgtcaagc cgaaacacga atttaattac cagcaggcgg ttgagcgtct
     2941 tcccggtgaa gatccggctc aactgaacga cccggcctac cgtcgtctgc gtatcatcac
     3001 cgataacctc aaacaggaag agcatgccat tgtccaggtg gaagaaatgc aggcggtgaa
     3061 tgccgtgctg tatggcaaat acaccatgga aggggatcag tttgatactg tcgaggtgga
     3121 ttttggacgc tctgaaggaa ataacattga gcaggccgac ggtaaaaaat ggtctgagca
     3181 ggaccgtgat acgtttgatc cgacgcatga tattgacctc tactgcgatc aggccagcgg
     3241 tcttgtgaat attgccatta tggacggtac tgtctggcgt ctgctgaatg gttttaagct
     3301 gttccgcgaa aaactggata cccgtcgcgg ctcaaattca caactcgaaa cggcagtgaa
     3361 agacctgggg gcggtggtgt ctttcaaagg gtattacggc gatctggcca ttgtggtggc
     3421 gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt tatctgccgg agggcacgct
     3481 ggtcctggga aatacggctg cagatgggat ccgttgttac ggtgccattc aggatgcgca
     3541 ggcgttgtcc gaaggtgtgg tggcttcttc ccgttacccg aaacactggc tgaccgtggg
     3601 cgatccggcc cgtgaattta ccatgacgca gtccgcaccg ctgatggtgc tgccggatcc
     3661 ggatgagttt gtggtggtgc aggtgaaata atccgtgagc gggggcgaaa tgcccccgtg
     3721 tcttttttca cagggggctg atatggcaac aaaagaagaa aatcagaaac atcttcgtca
     3781 actggctggc ctgctggggc gcgaggcgga tatgtcgggg agtgctgcgg atattgcgca
     3841 acgtgtgtct gagtgggaag aggagcttgc tgcttcccgg gagggcatta tgcctggtga
     3901 tgagagcggg cctgagcaaa atcacacaga cgatggtgag cagttgcaca acactgatgc
     3961 tacggatgat gttaaagcgg tccgtgtgcg gaaatgcctg catgtgatgg ggtattgccc
     4021 ggagacaggc cgtcccgttg aactgacgta ccggggcatg cgtgttctgg tgccatcacc
     4081 actggcgaca gccatgatac agcacggaac ggctgagcat gcgtgatttt cagaatgcct
     4141 ttgatgctgc ccttgccggg gtggacagca cgattgttga agtgatgggg ctctgtgcgc
     4201 agttcacctc gggggcacag tgtggcagcg aagttcaggg ggtttttgac gatccggagt
     4261 cgctggggtt tgccggtagc ggggtccgta ttgaaggaag ctgcccgtca ttatttgtac
     4321 ggacggatac ggttcgtgct gtgcggcgtg gtgacacgct gaccattaat ggtgagacat
     4381 tctgggtgga tcgtgtttct ccggatgacg ggggcagttg ttatctctgg ctcaaccgtg
     4441 ggcaaccacc ggcagttaac cggcgacgat aaacgcaggg tgaattatgg cgataaaagg
     4501 gcttgatcag gcgattgaaa atctgagccg ggttcgtaaa aacgccattc cggcggcttc
     4561 agcaatggcc attaaccgcg tggccacaac ggcgattaat cagtcttcgt cacaggttgc
     4621 ccgggagacc agggtgagcc ggaaactggt aaaggaacgg tccagactga aacgggccac
     4681 ggtcagaaat ccgaatgcca gaattatcgt taaccgcggt gatctcccgg tgattaagct
     4741 ggggatcagg atgccggggc gtcgtccgga cagcatactc aaagccggtc agcatcgtta
     4801 tcagcgggca tttattcagc gattaaaaaa tggtcgctgg catgtcatgc agcgtgtggc
     4861 cgggaaaaac cgttacccca ttgatgtggt gaaaatcccg atggcggccc cactgaaaca
     4921 ggcgtttgat gagaatgttg accgtatccg gcgtgaacgc ctgcccggag aactggcata
     4981 tgcgctgaaa caacaactga ggattgcgat aaaacgatga aacatactga tatccgtgct
     5041 gcagtgctgg atgcactgga gctgcatgaa cacggggcga cgctgtttga tggtcgcccc
     5101 gttgtttttg acgaagagga ttttcccgcg gtcgcggttt atctgacgga tgcagagtat
     5161 accggtgaag agctggatgc ggatacctgg cgggccacac tgcatattga agttttcctg
     5221 cctgctcagg tgccggattc agagctggat tcgtggatgg agtcccggat ttatccggcg
     5281 atgtccgcga tccctgcact ggcagggatg attaccacga tggttcagca gggctatgac
     5341 tatcgtcgtg atgacgatat ggcgttatgg agctctgcag atttgactta ttccattaca
     5401 tacgagatgt gaggacgata tgccaacacc aaatcccctg gcgccggtaa aaggtgccgg
     5461 taccactctg tgggtttaca ccggcaaggg tgatgcttat gcaaacccgt tgtcagatga
     5521 tgactggca
//
LOCUS       DAHBHC010000171         5242 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHBHC010000171 DAHBHC010000000
VERSION     DAHBHC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.175"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..94)
                     /locus_tag="M2A35_004953"
     CDS             complement(<1..94)
                     /locus_tag="M2A35_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCB7201130.1"
                     /translation="MAISDQTRSGDLAETFKSERETTKNQIRVAL"
     gene            complement(157..327)
                     /locus_tag="M2A35_004954"
     CDS             complement(157..327)
                     /locus_tag="M2A35_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201131.1"
                     /translation="MTTSNNDNSQQSKPTQAEQSMADYESSIMLEVPFDGLDYMTESV
                     KEPELDNFKQKK"
     gene            complement(331..885)
                     /locus_tag="M2A35_004955"
     CDS             complement(331..885)
                     /locus_tag="M2A35_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201132.1"
                     /translation="MSKCSPIDTTEEQKAIETIGADFISSYYKDRYEEAKSTYRRLED
                     KVNYLLAILAVETSALLAVFGTVDFKSGLKSNWFLTLSILCAYLCFVCIVMCFFFLWR
                     TWGLKDIPKMPIHKRKEEHDFLLYGDKIKTLRYHMFQYGKAVELIESLHKEKAVYARY
                     LFEGIALSFGFFILSVTFLLINKA"
     gene            complement(893..1240)
                     /locus_tag="M2A35_004956"
     CDS             complement(893..1240)
                     /locus_tag="M2A35_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201133.1"
                     /translation="MKRWIFSLLALVSVGVSANTITMQCGNFRMDAIPDSLFKINGET
                     VTSQKVKMLGKDGTGMQIKMELMPAKDGNNYGFEYIHRPGTKTRFLNVQLLQNSMDAP
                     KIIGSFPCKKVVG"
     gene            complement(1243..2307)
                     /locus_tag="M2A35_004957"
     CDS             complement(1243..2307)
                     /locus_tag="M2A35_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201134.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSASDVGTVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQAAVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTSQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAQDMPSSTALQRGL"
     gene            complement(2310..2612)
                     /locus_tag="M2A35_004958"
     CDS             complement(2310..2612)
                     /locus_tag="M2A35_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201135.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLVVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            complement(2612..3199)
                     /locus_tag="M2A35_004959"
     CDS             complement(2612..3199)
                     /locus_tag="M2A35_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201136.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLGLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            complement(3199..5187)
                     /locus_tag="M2A35_004960"
     CDS             complement(3199..5187)
                     /locus_tag="M2A35_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HCB7201137.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GTALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLENL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSDMPYYRANQYAQMLG
                     IDENTLMAVRRGVGGFSRQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSG
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKTTVNDLVKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQHAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKVTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
BASE COUNT         1249 a         1451 c         1173 g         1369 t
ORIGIN      
        1 gcaaggcgac gcggatctgg ttctttgttg tttcccgctc ggacttgaat gtttcggcaa
       61 ggtcaccgct acgagtctgg tctgatatgg ccatttgata aactcctgaa aacaaaaaac
      121 ccgccaagtg gcgggtctac aggaacaact ctaagtttat tttttctgtt taaaattgtc
      181 caactcaggt tctttaacgc tttctgtcat gtaatccaat ccatcaaaag gtacttcaag
      241 cattatactg ctttcataat cagccatgga ttgctctgct tgcgttggct tactttgctg
      301 tgagttgtca ttatttgatg ttgtcatcaa ctatgcctta tttatcaaaa gaaaagtaac
      361 cgataatatg aaaaaaccga aagataacgc aattccttca aaaagataac gagcataaac
      421 tgccttttct ttatgaagac tttcgatcaa ttcaacagct ttaccatact gaaacatatg
      481 gtatctaagc gttttaattt tatcaccgta aagtaaaaaa tcatgctctt cttttcgctt
      541 atgaataggc atttttggta tatctttcag tccccaagta cgccatagga aaaaaaagca
      601 cataacaata caaacaaagc atagatatgc acaaagtata gataatgtta aaaaccaatt
      661 actctttagc ccacttttga aatcaacagt cccaaataca gccagtagag cagaggtttc
      721 tacagctaaa atagcaagaa ggtagttaac tttgtcctca agacgacgat atgttgattt
      781 tgcttcctca tatctatctt tataatatga cgaaataaaa tcagcgccta tagtctcaat
      841 tgccttttgt tcttctgttg tatctattgg cgaacacttg ctcatttctt aatcaaccaa
      901 caactttctt acacgggaaa gagccaataa ttttcggagc atccatgctg ttctgcaaca
      961 actggacgtt caggaaacgc gttttagtgc ccggtcgatg gatatactcg aacccataat
     1021 tgttgccatc tttggcaggc atcaactcca ttttgatctg catgcctgta ccgtctttac
     1081 ccaacatttt tactttttgg gatgttactg tttcgccatt gattttaaat aatgagtcag
     1141 ggatcgcatc catacgaaag ttgccgcatt gcatcgttat ggtgtttgca cttacaccaa
     1201 cagacactaa cgccagcaat gaaaatatcc atcgtttcat ttctataatc ctctctgcaa
     1261 agcagtgctg gatggcatat cctgcgcacc gcgcgcttcg cacatcatat ccatgtacca
     1321 cgcctggccc cttgtgtcgc cagtgtacat aatcccgcgc acaatataaa cgccatccgt
     1381 tgcgatgctg gcaggctgcg atgtggtgcc gcttagcgtg atatttccat ccgtgttctg
     1441 gtcggtgatc tgaccaccag ccatcgcaat atcgttgttc gacaacgcgg tacgatacac
     1501 tgcagcctga tccagctgaa tgagtccgtt aacccggatg ttcggattaa taagcgcgcg
     1561 gacgtttacg ccgttaccga tagtctgctg cggcatgcca ataagcccgg tagcgctgtt
     1621 gagcacaatc gcttcatgaa catattcgtt attcgccacc atctggcgct gaccatccac
     1681 gaattgccat gttgcgccac attgcccggc tacgttatcc attagatgcc gcgtcatgcc
     1741 aaagagtacc cgcccccggg ggaatacagt agcaggcatt tcaggcgtca ggccttcggt
     1801 cgcgcctttg gcttcgaagt ctttcatcag cgcacggttt acatcagcga ccgtgtaacc
     1861 ggcagccagc gtctgtgagg ttatactggt ggcaaaagcc agatcagtat ctgctgcctg
     1921 aatcaggacg taggaatcaa ccggactgtc ttttcctgta accgagtagc gaatttcacc
     1981 gctgaaaatc agtccgtagt tgcgaccatc actctggccc acgtccgccg cgtcaacttc
     2041 ccgcactgtc ccgacgtcgc ttgccgacac ctccggcgcg ataccgtcgt aacccgcaat
     2101 cagacgcact ttcgaaaact cctgcccggt aattcggttc acagtatctg ccgagaggtt
     2161 ataaattttg atagtcccta cccgggacgc gctgctgatg ttgaaccagt cgatcgtaaa
     2221 ggtgactttg aaatcactta gctcaattcc ctgaccgttc ccgtccacaa gctgcagctc
     2281 gaaatgtctc atccagttct gtgacatgct tactccgttg ataccagtaa atggctgcga
     2341 ccgcccaggt cagtttttgt ggggtaatcc tgtgtgttgt catcacagac caccaccagc
     2401 ttaaaaccaa gccccataca ggcgtactgc gccagcaggt cagcaccagt gacaagagga
     2461 ataccggaga ttaccggctc ccctctgtcg ttctgcaggt ccataatcca gtacagatcg
     2521 cgccatatga tgctaatccg ccaggtgaca ccacccagga cgatgctgaa ctgctggttg
     2581 tccgctgtca gcggaatttc ctgaattgtc attagccgcc ccccagtaat gacgccacgt
     2641 tacccgtgat gcttttcagc agtgaagtat ctggaggctt tgtggttttg ttgccgctgt
     2701 tctgtaccgc cgacgtgctg gccccttcct tcatgttggt tttatccgcg acggtaatct
     2761 gctgtgtccg ggagataatg acctccctca gggtgaggac ggcggacagg acgttttcgg
     2821 ttgtcttgtc cgtcgtcact tccagcgccc ggatcaacat gttgctgtac agccgtttac
     2881 cggttaccac atcgaagggg atacggcttt cctgcagacc cagtagctcc tgatacgtct
     2941 gctgaggact caggccgagc aggctggtag ccgtcaggtt actggcaaaa tccagcaatg
     3001 cgccgccacc ggcgaaacca acctccatca ccacttctga cggtttttta taggcatgat
     3061 cagcgacagc ggccccgacc tctaccggat gctctgttat ttcaagcata tctgtatgct
     3121 tctctgaaat aacaacactg ggaacaatca ttcctatttt tctgctctgc tgatgaaaaa
     3181 gtgtagagag aatatccact aacccaccct cacctgatta cttcgcatga cctgagcatt
     3241 tgcagactgt tgccgacgtg caacctcatt accgacagcg tgcggatctc cgccaccgta
     3301 aatgtggtag gtattttgct ggttaacctc tgtcactttg ccactaattc ccgccacggc
     3361 agccttatta atcagctctc gagaatagat atttcttcca ttctcatgct ggataatgct
     3421 gctcatcaat gctgacatgg tttgcggatc gctcatattc agggcagccc ggggatccac
     3481 tcccagtcgt tgcgatacag ccctgatata cgcggttgtg ttgttattat cagacgcagg
     3541 tgcccaggta gagataattt tctccacact gtttattccc cgtccggcgt acagcattaa
     3601 ctgacgagca agagcccgta atccatcaaa agcagtttca aatctggcaa atcgcccgcc
     3661 cgggcgttca agagaagccc cagcctgacc agcaaaatta agatttcccg gattgttatt
     3721 ccgttctcct cgtttcgtag cctgtgcatg ttgttccggc tcatcatcac caaaccagcc
     3781 gcgtaccgtc cggcccacac tgcggggatc aaatccccag tgctctttaa tccagtcggc
     3841 agtactgtta gcgctgtctg taaccatcgg catcgctgac ggattttcgc tgccctgatt
     3901 aagtatctgt ttgccgatgc tgacggcatc agcccagcgg ccatctttga tagcgttgag
     3961 caggtcggcg atcatgttca gcattttgct gaattcgccc atctggtcga tgaagttgct
     4021 gaaatcccac ttcagggacc atgatttggg gtcaatattg agcagtttcg ccagcgcttt
     4081 caccaggtcg ttaacggtcg ttttaaggtc acgaaccatc ttcagcgcgg catcaacctc
     4141 cggtttccac ttgccccagt caatcaggct gtcgccgcct tccttccagg tctgatagtc
     4201 ctcccacagg agggcaatcc ccgccgccag cgcggtaatg aggccaatcg gcgacatcca
     4261 gaacgtactg ttcagaatgc gcagcgcaat cgtcagtgcg ccaaacagcg agatcaactc
     4321 ccgcgtttgc ttatccagcg attgccacca ggtgatgagg cctgatgtcc cctcaatcag
     4381 tctgaagaac aaccgcccga taatatcccc gagcgccaga atgcctttta tggctttcgt
     4441 cagggtctgc tcgatacgcg ggaagttgtc caggatatgg cggcgcagag tgtccagcga
     4501 acccgcaagc ccacccgcaa gattagagcc gattttgtca cgggccatgc ctgccatcgc
     4561 gccaaactca cgcagggagg tcataaattt gttggagctt ctggccgcct cgtcagcatt
     4621 gaagccgata gctttcgcca ttgcgctgta ctgcctggag aagccaccca caccgcgacg
     4681 cacagccatc agggtatttt cgtcaatgcc cagcatctgc gcatactggt tagcacggta
     4741 atacggcatg tcgctgagct tctggcctac ccctgtaaaa atagcggcca tgtcacgcat
     4801 gttaccgctg gcatcccgtg tctgtacccc cagacgattc agaaatccct ccgccccggg
     4861 attattacga ataaaccggg agaggttttc cagagaagat cgcgcagcgt ccacgctgcc
     4921 gccaacctgc gaaaccgcat agccaataga ctgaattccc tggactgtcg ctccggtgcg
     4981 ctgtgacgcc cagtaaagat tatccagacc ggaggcgatc ttagccgtga aggccaccac
     5041 ggacagcgca gttccttcga cggccagccc cattttgatg acatttgcag ttgtaccggc
     5101 gaggacagaa ccgaattttt tcgctcctgc atcatccaca ctgaagccaa gcgagacgag
     5161 gaaatcttta atagtttcag cgttcattat cctctctcca tttctcaatg cgccgctggt
     5221 tatccgcttt taccgccaga tg
//
LOCUS       DAHBHC010000172         5198 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHBHC010000172 DAHBHC010000000
VERSION     DAHBHC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.176"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..262
                     /locus_tag="M2A35_004961"
     CDS             <1..262
                     /locus_tag="M2A35_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="late control protein"
                     /protein_id="HCB7201138.1"
                     /translation="PVIEWPAVEPSLDAAKEAAPGLKGGAAKKEFSMTYSCPATLDLI
                     ALTAECKVTTEGFGSEEDREWTINTLSLTLAENGFSVRISME"
     gene            268..2097
                     /locus_tag="M2A35_004962"
     CDS             268..2097
                     /locus_tag="M2A35_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCB7201139.1"
                     /translation="MAKISGVYANGLGEPVPGINLVLTARATSSGVIMTTTAAQQTGA
                     GGEYCFELQPGVYVVTASGAYLGVITVNPDSVDGTLNAYLTNFSADEMTPAALAEVQE
                     LVATAQAAASVASGAATEARQNAAAAAAAAQISTAAAGSARFENFDEIININTTAMLE
                     LEKPEVLDPDITITITETINYDYVGPVNGFCDVASPLEYKIQMYAYTTGEYFNGETNL
                     TADGKFYFKRSWAGAKQFRLVRIADSKWITTLEYPLCIRSYWMPDDADPAVVKTMKDR
                     CYTYDQAVAAMALMVQQHDAVERYVAGLCALVEDAGGVKFYVNRLSAQSHRAYYRMGN
                     VAWVLYALAFYLHKFPTGDQVATVREKIAAGCTWVDTFRVITPGDPREGLYMGGSGKF
                     YDGGTVFDPDYIATWCALEHNVDIYFLFELLGRISGFESYKSKAADLAADIIEKFWIE
                     SEGRFRQGVHEDAPDNVAALDQSSWGGIFAAHVDPDKAVRCRKYMERFRYGTQECAGY
                     TPYDLDQGYSTQKRGVWCEGVGGVALFERLLGNEERAIELIAGMTPLRNKYGYRDSCM
                     DPTYDTLPNWPSTTNTGWMILCCQPSGFWNVNAPKLSIGIVRY"
     gene            2485..2649
                     /locus_tag="M2A35_004963"
     CDS             2485..2649
                     /locus_tag="M2A35_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HCB7201140.1"
                     /translation="MLRHMGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAKLLKCS
                     EFGDAIIKNM"
     gene            complement(2752..3075)
                     /gene="iraM"
                     /locus_tag="M2A35_004964"
     CDS             complement(2752..3075)
                     /gene="iraM"
                     /locus_tag="M2A35_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCB7201141.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3328..3372)
                     /locus_tag="M2A35_004965"
     CDS             complement(3328..3372)
                     /locus_tag="M2A35_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201142.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            complement(3608..3718)
                     /locus_tag="M2A35_004966"
     CDS             complement(3608..3718)
                     /locus_tag="M2A35_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201143.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            complement(3771..4175)
                     /locus_tag="M2A35_004967"
     CDS             complement(3771..4175)
                     /locus_tag="M2A35_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCB7201144.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(4396..5127)
                     /gene="bluR"
                     /locus_tag="M2A35_004968"
     CDS             complement(4396..5127)
                     /gene="bluR"
                     /locus_tag="M2A35_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCB7201145.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT         1462 a         1070 c         1213 g         1453 t
ORIGIN      
        1 tccggttatc gagtggcccg cggtcgagcc gtcgctcgac gcagcaaaag aagcggcacc
       61 agggctgaaa ggcggtgccg ctaaaaaaga attttcaatg acatactcat gcccggcaac
      121 gctggattta atcgcgctga cggcggaatg caaggtaacg acagagggtt ttggcagcga
      181 ggaagatcgg gagtggacga ttaacacgct atccctgacg ctggctgaga acggtttttc
      241 tgttcgtata tctatggagt gatcgcagtg gcaaaaatat caggggtgta cgcaaacgga
      301 ctcggcgagc cggtgccggg tatcaattta gtgttaacgg ctcgcgcaac cagttccggc
      361 gtgataatga caacgacggc agcgcagcaa actggcgcgg gcggtgagta ctgttttgaa
      421 ctccagccgg gtgtttacgt agtcaccgcc agtggtgcgt atctcggcgt gattaccgtt
      481 aacccggata gcgtcgacgg cacgttaaac gcgtatttga caaatttcag cgcggacgaa
      541 atgacgccag cggcgctggc ggaggttcag gagctcgttg ccacagcaca agccgccgcc
      601 agtgttgcca gcggtgccgc aacggaagcc aggcagaatg ccgccgccgc tgctgctgct
      661 gcgcagattt cgacggcagc ggcaggtagc gcccgatttg agaattttga cgaaattata
      721 aatattaata caacagcaat gctggagctg gaaaagccgg aggtgttaga tccggatatt
      781 actataacta tcacagagac gattaattat gactacgttg gtccggtaaa cggattttgc
      841 gacgttgcca gcccgcttga atataaaatt caaatgtacg cctacacaac aggcgaatat
      901 tttaacggcg aaacaaattt aacggcagac ggtaagtttt attttaagcg atcatgggcc
      961 ggtgcaaaac aattcaggtt ggtgcgcata gcagatagta aatggatcac tacgctggaa
     1021 tatccattgt gtattagaag ctactggatg cccgatgatg ccgatcctgc ggtcgtcaaa
     1081 acaatgaaag atcgttgcta tacatatgat caagcggtcg ccgccatggc attaatggtc
     1141 cagcagcatg acgcggtgga acgctacgtc gccgggttat gcgcgctggt agaagacgct
     1201 ggcggggtta aattttacgt taacaggcta tcagctcaat cgcaccgagc ttattacagg
     1261 atgggtaacg tggcctgggt tttatatgct ctcgcattct atctgcataa attccccact
     1321 ggtgaccagg ttgctactgt tcgtgagaaa atagcggctg gatgcacttg ggttgatacg
     1381 ttcagagtta tcacccccgg agatccgcgc gaggggctat atatgggggg gagtggtaaa
     1441 ttttacgacg gcggcaccgt ttttgatccg gactatatag caacgtggtg cgcacttgag
     1501 cataacgttg atatttattt tctgttcgaa ctactgggca gaatatcggg attcgagagt
     1561 tacaaaagca aggcggcaga tctggccgcc gatattattg aaaaattctg gattgagagc
     1621 gaagggcgtt ttcgccaagg ggtgcacgaa gacgccccgg acaacgttgc tgcgttagat
     1681 caatcgagtt ggggcgggat ttttgctgca cacgttgatc ctgacaaggc cgtgaggtgc
     1741 aggaaataca tggaaagatt tagatacggc acacaggaat gcgctggata tacgccatat
     1801 gatcttgacc aggggtattc cacacaaaaa cgcggcgtgt ggtgcgaggg ggtgggtggt
     1861 gtcgcgctat ttgagcgcct gctcggtaat gaggaacgcg caattgaatt gatagcagga
     1921 atgaccccac tcagaaacaa atacgggtat cgcgattcat gcatggatcc aacatacgac
     1981 acgctgccaa attggccctc aacgacaaac accggctgga tgattttatg ttgtcagccc
     2041 agtggttttt ggaacgtaaa cgccccaaaa ctaagcattg ggatcgtgag atattgaggc
     2101 aaattgtcgg gagtttttcc ccatttattc cccaataatt ccccgtacaa aaacaggcat
     2161 aaaaaaacca gccgtaacag gctggttctt aaggtgtttt tggtcggcac gagaggattt
     2221 gaacctccga cccccgacac cccatgacgg tgcggggcgt tcggtgtttg cattttgcaa
     2281 ttttgcgaaa atgtaaatta attgtattaa tgcgtcatta tctgcagatt tataagcctg
     2341 ccccgatgaa aaagcggtta tcaaaactca aaaagagtta tcgcaaaaca agcacttgca
     2401 aaccatctca aatgatgtga tttattaaaa tatttcctga tattttaata aatatcagtt
     2461 agttgaaatt aaaccatcaa actactgtta cgccatatgg gctggactga agccgcagac
     2521 ctgattgtta aaggtatgga aggcgcaatc aatgcgaaga ccgtaaccta tgacttcgag
     2581 cgtctgatgg aaggcgctaa actgctgaaa tgttcagaat ttggtgatgc gatcatcaag
     2641 aacatgtaat cactacatgt gtttaatatt gcaacgggcg tataacacgc ccgttgtttt
     2701 atttatgtag gtattattaa tagcatatcg agcatattta tataaaaccc tttacttgag
     2761 cccatatggg catattttta taatgcaact attatgcaaa catttatttg ttatttttct
     2821 ttctcctgga ggacactctt gactgctttt gagtagactc cataaatcct tgttgaatgg
     2881 tgcgatgtta taaatagtaa taggatattc tttatcttta aggataattc cagatttaac
     2941 cggtgtaaat atactgccag gagggagaaa tatagtagat tgataccaga tgatcatttt
     3001 catattaccc catatggctg aaaaagatat gccgcatgaa ggttgaatta tcgtgtcaat
     3061 tactatccac ttcatttgtt atgtcttatc ccacggtatt taatatagtt catttggatg
     3121 ttcatttctt tattttgcat atgagtatat taccccttca aaaaataaat taattaaaac
     3181 gattgcttat ataaaacaaa atttaaagca aggaatctca atggatgtta aacaaaatga
     3241 gatttagtga aaacaataaa ttattcactt cgttttagat ttgtttagct ataatgttat
     3301 acattcaaat gactgaacat cctgtaatta aaacatagcc tttatgctac tttgtgccaa
     3361 tttgctaaac attatggttg cctttttata taacgataat aatgaatata agcatgacat
     3421 gagaataagg tttcaatttt tgagttatat aggaaaggtt taacctgttc ctggctaaaa
     3481 tacatataac tggatgatga ctaaaccaaa acacatgtgc gttaagtatt gaaacgggcg
     3541 tatggcacac ccgttgtttt tataaatata ttaaccgtta taaaataacg tatagaaagt
     3601 caagtgatca catttcaaat atcaatttga tagtattggc atggtgatta tttatgggta
     3661 gcaataaaag gacagtattt atcatccata gggatagtct ctgtactttt attcccatta
     3721 tgctaatgcc ttactgaatt atgaagcatt tcttaagcat ccaactttag ctagattaat
     3781 ggtttattat tttctacatc ttcaatatat aaaagcgtat tatcaatggc gtagtaactg
     3841 cgtttgttat gattaacatc agtaacccac cggaaaacgc ccgcgcctgc tagtgttgaa
     3901 cagtattccc gaaatgtaga ttttccgcaa atatgaagca atgcggcctc ttttatttta
     3961 gcagggttct tggtcgtact aacttttaac aggttcctgg ttcctcttaa taacaaaacc
     4021 gtgtcatcgt gagtaataat tctgatgtta tccgtagcca gataataaat gtaatgtgca
     4081 atacggtgat gttttaattc tgaataaaac caggagaagt tttgctcttt tctcacttgc
     4141 tcaaacatct tttgaaaaac aacgacctga tccatcagga taataacctc ttgttagttg
     4201 tgagactgcg tagtgtgcac gatcggtttt accacttcaa tctggtctgt cctttggctg
     4261 tgatatgtac agagtgtgat agagggaata tctgaattct cccggtgagc attttgcaac
     4321 ggaccagctc cggtacaaac gctgttgtgg gttcagatta taacattctg tctaaggggc
     4381 gggataaagg tgaaattagg gggcatgaaa gatgacttta taaccttgct caccccagtg
     4441 ttgtaaaagt tcgttttgcc ttctcgttgg tgccatgcct gtccagacaa tcaatgtttg
     4501 cgtcgggaac agttccgggc gcggcgattc aatgggatca gcaagaacag aaatgtgcca
     4561 tcctgacaat gataaacgcc atgcttccag ccacaatcgt gctctctctt caacattcca
     4621 cgccatcaga atggcttctt taccgggctt acggcgcatt tcgaaaagcg aggttgctgc
     4681 gtattcaatt aatgcgccgt caaacatact gctcataatg cgggaggtgt tgtgatcaag
     4741 cacgagacgc tggcgaacag gaaggtaaac atgattaatc aattgatcta ctgggtactc
     4801 tcgacccagt gaaataattc tcgcgcgtag tttggcagga ttagccatgc gaagaattga
     4861 catcatctct tcttgcaggc ggctccagtc atcttccgta tcctggctgg tggtttccag
     4921 taatgcttta actttgccta cagggacgcc attacttatc caacgcttga tctcttcgat
     4981 gcgttgtatg tcttcttcat caaagagtcg gtgtccgcct tcactgcgct gtggttttaa
     5041 caaaccgtag cggcgttgcc aggcccggag agtgacagga ttaatcccgc aacgttcagc
     5101 aacatcacca atgctgtaat aagccacaat tcctccttgc ggtcacaaat ctccgtcgcc
     5161 tgtacacgac ccaataatac tttgtacaat atacgcta
//
LOCUS       DAHBHC010000173         5145 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHBHC010000173 DAHBHC010000000
VERSION     DAHBHC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.177"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..186
                     /locus_tag="M2A35_004969"
     CDS             <1..186
                     /locus_tag="M2A35_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCB7201146.1"
                     /translation="AADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLDAPELLD
                     FAQQYACQKGLTATLRG"
     gene            192..2624
                     /locus_tag="M2A35_004970"
     CDS             192..2624
                     /locus_tag="M2A35_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCB7201147.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNNDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            2770..3585
                     /gene="ybiV"
                     /locus_tag="M2A35_004971"
     CDS             2770..3585
                     /gene="ybiV"
                     /locus_tag="M2A35_004971"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCB7201148.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            3737..5002
                     /locus_tag="M2A35_004972"
     CDS             3737..5002
                     /locus_tag="M2A35_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCB7201149.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAHLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         1271 a         1351 c         1345 g         1178 t
ORIGIN      
        1 gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac gctgggcatc
       61 aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca
      121 gaactgctcg actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga
      181 ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca
      241 aaaatgcgct ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata
      301 ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg
      361 cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc
      421 aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa
      481 aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca
      541 aacgcgttct gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct
      601 acggcatgtt taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag
      661 gcaatatgac ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag
      721 gtcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc
      781 tggaagattt acatggcgaa caattcctga aagcgattga tatcgtgctg gtggcagtca
      841 gtgaacacat tgaacgtttc gctgccctgg cgcgtgaaat ggctgctacc gaaactcgcg
      901 aaagccgtcg cgatgaactg ctgacgatgg cagaaaactg cgatcttatc gcccaccagc
      961 cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccaattg attttgcaga
     1021 tcgaatctaa cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact
     1081 atcgccgcga cgttgaactc aaccagacgc tggatcgcga acacgccatc gagatgctgc
     1141 atagctgctg gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag
     1201 cctctgcggg aagtccgctg tatcagaacg ttaccattgg cggacaaaat ctggttgatg
     1261 gtcaaccaat ggatgcagtg aatcccctct cttacgcgat cctcgaatcc tgcggtcgcc
     1321 tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc
     1381 tcgacgcctg cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg
     1441 aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg
     1501 cagcgattgg ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca
     1561 tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca
     1621 ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt
     1681 tcgatgaagt aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg
     1741 aaatcgaata tgtcgtcgac accatgctgg aagagaacgt acacgatatt ctctgctcgg
     1801 cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg
     1861 actgggtttc tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga
     1921 agaaactggt atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg
     1981 atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga
     2041 agtacggcaa caacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata
     2101 tcgacgaact gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact
     2161 attacgcggg tacctcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa
     2221 caccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg gcctctggta
     2281 ctgaccatct cggccctact gcggtcattg gctcggtggg taaactgcct acggcagcga
     2341 ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg
     2401 acaagcagaa actgatgatc ctgctgcgca ccttctttga agtgcataaa ggctggcata
     2461 ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt
     2521 accgcgatct ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag
     2581 acgcacagga cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg
     2641 gcgtcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat
     2701 ataatttgtg ctcttcgtca cattgttatt acactgttac ggtttcattt tcgagccagg
     2761 agcacaccta tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac
     2821 gccaaaacgt acaaccaacc acattttatg gcgcaatatc aggaactgaa aaagcgcggc
     2881 attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag
     2941 ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag
     3001 caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta
     3061 aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat
     3121 gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa
     3181 gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa
     3241 atcccgttag tgatcgacaa actgcacata gcgctcgatg gcattatgaa acccgtcacc
     3301 agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg
     3361 cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt
     3421 aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa
     3481 aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat
     3541 gtgattcagg cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc
     3601 cgtgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt
     3661 gcattaactt atttataact tatattgtca catgcaaatc agaatggttt cgaatgaaag
     3721 ttaatgagag gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa
     3781 agcagctatc cgtcagatga agcacgcgct gcgggcgcat cttggcgacg tccagcagat
     3841 ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc
     3901 acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac
     3961 tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc
     4021 ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt
     4081 tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc
     4141 cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat
     4201 ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg
     4261 gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac
     4321 gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc
     4381 agcgtatcag cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg
     4441 gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc
     4501 cgtgtttcgg acattccagg gctggacagc gctctctgat atgctgcctg gtcaggggtt
     4561 gctgcacgtt gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga
     4621 tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga
     4681 gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga
     4741 ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg
     4801 ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc
     4861 gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga
     4921 ctatgaaaca aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg
     4981 agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga ttaagaaaat
     5041 cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga tgcgctacgc
     5101 ttatctggcc tacaaagtcc tgcagattca atatattaca ggtac
//
LOCUS       DAHBHC010000174         5108 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHBHC010000174 DAHBHC010000000
VERSION     DAHBHC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.178"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..587
                     /locus_tag="M2A35_004973"
     CDS             <1..587
                     /locus_tag="M2A35_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7201150.1"
                     /translation="SNKELGELLQALDKKIFPPYYSALIRLLIVFGCRTGELRLSEIR
                     EWDFKEMLWTVPKEHSKTKVAIFRPIPESILPFVTQLVEQNRHTGLLLGELKQEASVS
                     QYGRFTHKKLNHPHWSLHDIRRTFTTMLNDLGVDPHIVEQLTAHQMPGMQRVYNHSRY
                     IDAKRNALDMWTERLEILAGAHENVTTLPVAREI"
     gene            941..1129
                     /locus_tag="M2A35_004974"
     CDS             941..1129
                     /locus_tag="M2A35_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCB7201151.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKTGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSREAVH"
     gene            1182..2489
                     /locus_tag="M2A35_004975"
     CDS             1182..2489
                     /locus_tag="M2A35_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCB7201152.1"
                     /translation="MNTGYTPVQGRGFVRPEKQNLQNFAVISGLIGGSETNIVNARAL
                     QMFDDEKGVNLTYPPDGNQNMSIISESVFYKLIKTKSAPLPERPCEQLTYCAKNESVR
                     GDYINRGRNDHSCRITGKTKATAKGRQWVLRQIKLERITNNATFAAGGQCDQCAGLLV
                     GYSCSLALLRNRAVSACFLASSASLATSSGERSSDALSILARPSLTVIFLGSSIITCA
                     SLWLLLIKAVTEQSVTEKKFRLGYSCSLALRRWRRLISPSSAETINCPVLSPSSFKFS
                     TASAIAWGTRASSFLDFPLTAFVAISDFLVIRWLTVYTQENGKTLLTWLTPVTSIVAD
                     TFIEVIDEKTKPAGATNTNGPLTTNDNESIEVAMLNHTTHPQGRDSHNLNKYIWRFIA
                     LSTAQPRVITIEATSEQEARQQSPDGCVMVFAARIRQGVRHVQ"
     gene            2479..2739
                     /locus_tag="M2A35_004976"
     CDS             2479..2739
                     /locus_tag="M2A35_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201153.1"
                     /translation="MCNNTRLDAAAEAIKTLIDALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAGKILTADIQGGAPCMMTVILNQ"
     gene            2709..2936
                     /locus_tag="M2A35_004977"
     CDS             2709..2936
                     /locus_tag="M2A35_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201154.1"
                     /translation="MHDDRYFKSMSHAENALTNSECLRQILLIWLDGLSDAPEDERES
                     NLAGAFISLLDTVIVELNKAIEIHDKKINAE"
     gene            2942..3229
                     /locus_tag="M2A35_004978"
     CDS             2942..3229
                     /locus_tag="M2A35_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCB7201155.1"
                     /translation="MKKKNSGFTASGPARPEFMNGDIYRDKYGGMVTIKGVAERRITY
                     RRDGYEYDCVMPVYQFRRDFTLVGHRKAVNQKRAASYIRKIREMLVAGGKK"
     gene            3226..>5108
                     /locus_tag="M2A35_004979"
     CDS             3226..>5108
                     /locus_tag="M2A35_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCB7201156.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDDVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHGQKCENWRRYLNVERQNLSADLVIKLPVKKKTTTSPGDDELKPRVESRAD
                     GVFWVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKPANHEVITMAI
                     PCGGIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGNHEEWQLSTTTGWHFGAYI
                     MPDGSVIGDCEKPVLFTGKTAAVNGYSVAGTAEGWRDTVARLAGGNPSMMLGVAVSLS
                     APLIGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEA
                     HNDGLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEM
                     DVETFLKTEGIKIKAGQLVRLLNVPMEKATQFHEYSTGKAHADALKDAWTENHGAAGR
                     EWVKWLAEHQQEAKDTVRTCRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTG
                     WDEQECRDAIQHNFNAWVKEFGTGNREFKQMVEQAEAFLASFGFSRYLPWPNSDERDL
                     PIK"
BASE COUNT         1446 a         1170 c         1339 g         1153 t
ORIGIN      
        1 ttagtaacaa agaactgggc gaattattac aggcgctgga taaaaaaata tttccgccct
       61 actacagcgc actaatccgc ctcctgattg tgttcggctg ccgaaccgga gaactgagat
      121 tatcggagat cagggagtgg gattttaagg aaatgctctg gacagtaccg aaagagcaca
      181 gcaaaacgaa ggtcgccata ttcaggccaa taccggaaag cattttgccg ttcgtcacac
      241 agctggtgga gcagaacagg cacacagggc tattactcgg ggaactgaaa caggaagcca
      301 gcgtgtcgca atacggcaga ttcacgcaca aaaaattaaa ccatccgcac tggtcactac
      361 acgatatacg gcgcacgttt accacgatgc tgaacgattt aggcgtggat ccgcatattg
      421 tcgagcaact taccgcccac cagatgccag ggatgcaacg agtctataac cactcccgct
      481 atattgatgc gaaacgtaat gcgctggata tgtggacaga gcgattagag atactggcag
      541 gtgctcacga aaacgtaacc acactgccag tagccagaga aatataattt ttttcgcgat
      601 tttttagaac ggaaacaacg tgaaacaacg agacaccacg acgaacaacg gcgaacaatc
      661 tggaacaaac ggattaaaaa tcaaacagcg ccataaacat ttgattttta tatgtatact
      721 gtttttttat acactggcga atcactctta aaaacatgta aaaaagacgt aaaaacagaa
      781 ttttactcta ctttatcaca atgatttaaa agtattttta ttctaattac cccatttctg
      841 taaaatgacc gtcagcaata tttgccattc atcactccag cgggaacaat acgacacaat
      901 aagacaccaa atgactcttt gagaaatgac ggaggcaata gtgttaagca ctgatcgctt
      961 tatacgtgaa aaagaatgtc agcaacttac aggcctgagc cgctcatgtc ggtaccgcct
     1021 ggaaaaaacc ggacaattcc catcacggcg taaactcggc ggtcgttccg ttggctggtc
     1081 tttatccgaa gttctggcat ggaaggacag ccgcgaggca gttcactaat caggctggcg
     1141 gcacacagcc gcctcccatc cactaataca gagatttaac catgaatact ggatatacgc
     1201 ctgtacaggg gcggggcttc gttcggcctg aaaaacagaa tctgcaaaat ttcgcggtta
     1261 tttccggcct tattggcggg agtgaaacca atattgttaa cgccagagcg ttgcagatgt
     1321 ttgatgatga aaagggagta aatttaactt acccccctga cggcaatcag aatatgagca
     1381 ttatctctga gtcagttttc tacaaactaa taaaaacaaa aagcgccccg ttgccggagc
     1441 gcccttgcga acaattaacc tactgcgcaa aaaatgaatc tgtgcggggg gattatatca
     1501 accgtggtcg aaatgaccat agttgcagga taacaggcaa aacaaaggcg accgcaaagg
     1561 gtcgccagtg ggtattacgg caaatcaaac ttgaacgcat caccaacaat gccacatttg
     1621 cggctggtgg gcaatgtgat cagtgtgctg gcttgctggt gggctattcc tgctctttgg
     1681 ctttgttgcg taatcgggct gtttcggctt gttttcttgc atcctctgcc agtctcgcca
     1741 cttcatcggg agaaagaagc tcagatgctc tcagcatttt agccaggcca tcgttaacag
     1801 taattttttt aggatcatca attatcacat gtgcctcctt gtggttgctg ttaataaaag
     1861 ctgtgacgga gcaaagcgta accgaaaaaa aattccggtt gggctattcc tgctctttgg
     1921 ccttgcgccg ctggcggcgt ttgatctcgc cttcaagtgc cgaaacgata aattgcccag
     1981 tgctttcgcc ttcttccttt aaattttcta cagcgtcagc tattgcatgg gggactcgtg
     2041 cctcaagttt tttggatttt ccattaactg ctttcgttgc catatctgat ttccttgtta
     2101 ttaggtggct gacagtatac acacaagaaa atggaaaaac actattgacg tggctgacac
     2161 ctgttactag tatagtggct gacaccttta ttgaggtaat cgatgaaaag acaaagcccg
     2221 caggtgctac caacaccaac gggcctctaa ccaccaacga taacgagagt atcgaggtag
     2281 ctatgttaaa tcataccaca cacccgcaag ggcgggactc gcacaacctg aataaataca
     2341 tctggcgttt tatcgccctg agcacggcac agccgcgcgt gattaccatt gaggccacca
     2401 gcgaacagga agcacgccag caatctcctg atggctgtgt gatggtattc gccgcccgta
     2461 ttcgtcaggg ggtacgccat gtgcaataac acccgtctgg acgcagccgc cgaagccatc
     2521 aaaacactga ttgatgcgct gattgatatt tctgttatcg cagacagggc gcataagcac
     2581 gctaccagcg aaacagaata tgccggggct ttcgttcctc actcactggc tgttatgcaa
     2641 cttagtgctg atatggcgct gaatgaggca ggcaaaattc ttaccgcaga tattcaaggg
     2701 ggcgcaccat gcatgatgac cgttatttta aatcaatgag ccatgcagaa aatgcgctga
     2761 ctaacagtga atgcttgcgg caaatattgc ttatctggct tgatggttta agtgatgcgc
     2821 cggaagatga acgtgaatct aatcttgctg gtgcgtttat ttcattactc gatacggtaa
     2881 tcgtcgaatt aaataaagcg atcgaaattc acgataaaaa aataaacgcg gaataaaaac
     2941 catgaaaaag aaaaattctg gctttactgc cagcggcccc gctcgtcctg aatttatgaa
     3001 cggcgatatt taccgcgata aatatggcgg catggtaacg attaaaggcg tggcagaacg
     3061 gcgcatcact taccgccgtg atggctacga atatgactgc gtgatgcctg tttatcagtt
     3121 ccggcgtgat ttcacgctgg tgggccatcg taaagccgta aatcagaaac gtgcagcaag
     3181 ctatatccgc aaaatccgtg aaatgctggt cgcagggggt aagaaatgaa actggcaccg
     3241 aacgtaaaaa aacaaccacg cggaataaaa cacaaagaca cagaggtaat tattttcgcg
     3301 ggtagtgatg cgtgggcaca tgcaaaacag tggcaggagc aggacggccc cgcatctggc
     3361 gatgatgtgc cgcctgtgtg gcttgggcca aatcagcttg ccgaacttga tgcgctgaaa
     3421 attgttccgg atgggaaaaa acgcgtaagg ctgtaccagg ccggagaact ggatttagtg
     3481 gagaccaaaa agatcggtca gaagctggcg gcggcagata ttcaggatgc gaatttttac
     3541 cctgaaggaa tgcacggtca gaaatgtgag aactggcggc gctatctgaa cgttgagcgt
     3601 cagaatcttt ctgcagatct ggtaattaag cttccggtaa agaaaaaaac caccacaagc
     3661 ccgggtgatg atgaattaaa gccccgcgtt gaaagccgtg ccgatggtgt tttctgggta
     3721 acgccaaaag tggataagca gtcaggcgaa attatccggc ctgagacgtg gttatgttcc
     3781 ccgcttgaac tactgggaac ggggacaatc ggcaaagagc attaccgcgt gatgcgctgg
     3841 aaaaaaccag caaaccatga agtcatcaca atggcgatcc cgtgtggggg cattggcgac
     3901 cgtgacggct ggcggctgct gaaagaccac ggactgaatg tgacaaccaa cggtaaatac
     3961 agggctatcc tggctgactg gatgcagtta agcggaaacc atgaggaatg gcagttaagc
     4021 acaacaacgg gctggcattt tggcgcgtac atcatgccgg acggttcggt cattggtgat
     4081 tgcgaaaagc cagtcctgtt taccggaaaa actgccgctg ttaatggtta ttccgtggca
     4141 ggaacggcgg agggctggcg cgataccgtt gcgcggttgg ctggtggtaa tccgtccatg
     4201 atgctgggtg tggcggtatc gttatcagca ccattaatcg ggctggtggg cgctgacggc
     4261 ttcggggtac atcttttcga acagtcatcg gcaggtaaga ccacaacgca gaacatcgca
     4321 tcaagtttat ggggagagcc ggacgcgcag cggctgacct ggtacggcac agcgttaggt
     4381 atcgccaacg aagcagaggc acacaatgac gggcttttac ccctggatga aataggccag
     4441 gccggaaacg cgcgggaggt gtccacgtca gcctatacgc tgtttaacgg ttccgggaaa
     4501 ttacaggggg cgaaggacgg cggcaacagg gagataaaac actggcgcac ggtggcaatc
     4561 agtaccggag aaatggacgt tgaaacattc ctcaaaacgg aagggataaa aattaaagcg
     4621 gggcagcttg tccgtctgct taacgtgccg atggaaaaag ccacacaatt tcacgaatac
     4681 agcaccggaa aggcgcacgc agacgcgtta aaggatgcct ggacagaaaa tcacggggca
     4741 gcgggtcgtg agtgggttaa atggctggca gaacaccagc aggaggcaaa ggacacggta
     4801 aggacatgcc gtgagcggtg gcgcaacctg ataccggaga gctacggcga acaggttcac
     4861 cgtgtggggg agcgatttgc catactggag gccgcgcttg tgctttcagg tcatgttact
     4921 ggctgggatg agcaggaatg ccgcgatgcc atacagcata attttaatgc ctgggtgaag
     4981 gagttcggca cgggtaacag ggagtttaaa cagatggttg aacaggcaga ggctttttta
     5041 gcgtcgttcg gattcagcag ataccttccc tggcccaact ctgacgagcg tgatttaccg
     5101 attaaaga
//
LOCUS       DAHBHC010000175         5097 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHBHC010000175 DAHBHC010000000
VERSION     DAHBHC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.179"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..146
                     /locus_tag="M2A35_004980"
     CDS             <1..146
                     /locus_tag="M2A35_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HCB7201157.1"
                     /translation="ARINRGETIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKG
                     ASV"
     gene            143..445
                     /locus_tag="M2A35_004981"
     CDS             143..445
                     /locus_tag="M2A35_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HCB7201158.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            513..845
                     /gene="emrE"
                     /locus_tag="M2A35_004982"
     CDS             513..845
                     /gene="emrE"
                     /locus_tag="M2A35_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCB7201159.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            893..1042
                     /locus_tag="M2A35_004983"
     CDS             893..1042
                     /locus_tag="M2A35_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201160.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            1100..2626
                     /locus_tag="M2A35_004984"
     CDS             1100..2626
                     /locus_tag="M2A35_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7201161.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VALSVAPEVTAIAEKIRLLDKELRRASVSLKTLKSKGVNSFSDFYAIDLTSKNGRELC
                     RTLAYKTFEKIIINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDGEIYF
                     "
     gene            3091..3642
                     /gene="ybcL"
                     /locus_tag="M2A35_004985"
     CDS             3091..3642
                     /gene="ybcL"
                     /locus_tag="M2A35_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCB7201162.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATITYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            3652..4449
                     /locus_tag="M2A35_004986"
     CDS             3652..4449
                     /locus_tag="M2A35_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201163.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            4566..4667
                     /locus_tag="M2A35_004987"
     CDS             4566..4667
                     /locus_tag="M2A35_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201164.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            4664..>5097
                     /gene="ybcN"
                     /locus_tag="M2A35_004988"
     CDS             4664..>5097
                     /gene="ybcN"
                     /locus_tag="M2A35_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HCB7201165.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKLTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLL"
BASE COUNT         1597 a          874 c         1114 g         1512 t
ORIGIN      
        1 cagcgcgaat taaccgtggt gagacgatac ctgaaccagt aaaacaactt cctgttatgg
       61 gcggtagacc tctaaatcgt gcacaggctc tggcgaagat cgcagaaatt aaagctaagt
      121 tcggactgaa aggagcaagt gtatgacggg caaagaggca attattcatt acctagggac
      181 gcataagagc ttctgtgcac aggacgttgc cgcggtaaca ggcgcaaccg taaccagcat
      241 aaatcaggct gcggctaaaa tggcgcgggc aggaatcctg gtcgtggatg gtaaggtctg
      301 gcgaacggtg tattaccggt tcgctaccag agaagaacgg gaaggaaagg tgagcacgaa
      361 tctgattttt aaggagtgtc gccagagtgc cgcgatgaaa cgggtattga gggtatataa
      421 aagaacatca atgggtacac aatgatgaaa caggtgagtt gagttcaaac tgtagtacaa
      481 ttctctccag tttgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggtg
      541 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagaaggt tttacacggt
      601 tatggccatc tgttggtaca attatttgtt attgtgcatc attctggtta ttagctcaga
      661 cgctggctta tattcctaca gggattgctt atgctatctg gtcaggagtc ggtattgtcc
      721 tgattagctt actgtcatgg ggacttttcg gccaacggct ggacctgcca gccattatag
      781 gcatgatgtt gatttgtgcc ggtgtgttgg ttattaattt attgtcacga agcacaccac
      841 attaaaataa tttgtttcta aacgactaaa atatggaggc tcttatattt atatgagcct
      901 cgttttatgc tttttgttaa tgtctttatt ttttatgtat tcttttgtgc tttcaagatt
      961 atggcgtaag aaaattgcaa tacgattatt gttgtatatt caagataatg tgaccttaat
     1021 tgtcttttta aataaaaatt aaacaaaaat tatatcccac cactaaggtt tataaaagca
     1081 tacgttagca ggtgtcacca tgaaaaaagc catagcatat atgcgatttt catcaccagg
     1141 tcagatgtct ggtgactcat taaaccgaca gagaagactt attgctgaat ggttaaaggt
     1201 aaatagtgat tattatcttg ataccataac atatgaagat ttaggattaa gtgcattcaa
     1261 aggaaagcat gcacaatcag gagctttttc ggaattttta gatgctatag agcatggtta
     1321 tatattgcca ggaactacat tgttagttga aagtctggac agactttcaa gagaaaaagt
     1381 cggtgaagcg attgaacgtc tgaaattgat tttgaatcac ggtattgatg ttataactct
     1441 ttgcgacaat acagtctata atattgactc tttgaatgag ccatattcat taataaaagc
     1501 catacttata gcacaaaggg caaatgaaga aagcgagata aagtcaagtc gggttaaatt
     1561 atcatggaag aaaaaacggc aggatgcact ggaatcaggt acgattatga cggcgtcttg
     1621 tccgagatgg ctctccttag atgacaaaag aacggctttt gttccagacc ccgacagggt
     1681 gaaaactatt gagctaattt ttaaactcag gatggaaagg cgctcattga atgcaatagc
     1741 caagtattta aatgatcatg ctgtaaagaa tttctcagga aaagaaagtg catggggacc
     1801 ttctgtaatt gaaaaattat tagcgaataa agctctgata ggtatatgcg taccttcata
     1861 tcgtgcaaga gggaaaggga taagtgaaat cgctggctat tatcccagag tcatatcaga
     1921 tgatttgttt tacgctgtac aggaaattcg gttggcacct tttggtatta gcaatagtag
     1981 caagaatcct atgctaataa atctacttcg aacagttatg aagtgtgagg cttgtggtaa
     2041 taccatgatt gttcatgcgg tatctggaag tttgcatggc tattatgttt gtccgatgag
     2101 aagattacat cgatgtgaca ggccatcaat aaaaagagat ttggttgatt ataatatcat
     2161 taatgaattg ctttttaatt gtagcaaaat tcaaccagtt gaaaacaaga aagatgctaa
     2221 tgaaacttta gagttaaaaa ttattgagct tcagatgaaa attaataatt taatcgttgc
     2281 attgtctgtc gcgcctgaag ttaccgctat agcagagaaa ataagactat tagataagga
     2341 attacgaagg gcttcggtat cattgaaaac tttgaagagt aaaggtgtaa attcattcag
     2401 tgatttttat gctattgact taaccagtaa aaatggacga gagttatgcc gtacacttgc
     2461 ctataaaaca ttcgaaaaaa tcataattaa tacggataat aaaacctgtg atatctattt
     2521 tatgaatggc attgttttta aacactatcc tttaatgaaa gtaatatccg cccagcaggc
     2581 gataagtgct ctcaaatata tggttgatgg tgagatttat ttctaaataa tgatctcgga
     2641 ttttaagtta tgctatggtg ataaagtgca agacagaatt aattatcttt gacgaaactt
     2701 aatgggtaat tactttgttt gctcccacaa gcgagttttg tacggctgta ttggggtagt
     2761 aaatgagcta tacaatctta atcatttgtt aggtgagaac tcttggtcgc agattcaaat
     2821 actgaaaata cgtgacaaat tattatgagc aaaatggtgt atgtcacgta ttttgaatga
     2881 taggttaaaa aataacaccg actttcgtag gtattactaa taataaagca gagtttttag
     2941 atagtatcaa tgtgctttgt gtatattgtg gcaaataatt gggttggggg tacaattgtg
     3001 attgcttttg cataaaaaat gcgcctttat gcataatgag ataaaggaat atcaaataaa
     3061 ataacgatag gtcataacaa agaggttttt atgaaaacac ttatcgtttc aactgtattg
     3121 gcattcataa cattttctgc gcaggctgca gcatttcagg tcactagtaa tgaaataaaa
     3181 acaggagagc aacttacaac gtctcatgtc ttttctggat ttgggtgtga aggtggtaat
     3241 acatcgccct cattaacctg gtctggtgtt cctgaaggta ccaaaagctt tgccgtaact
     3301 gtatatgatc cagatgcacc tacaggcagt ggttggtggc attggactgt tgttaatatt
     3361 ccagcaacaa taacatattt gcccgttgat gcagggagac gtgatggaac aaaactgccg
     3421 actggtgctg ttcaaggccg aaatgatttt ggctatgctg ggtttggtgg ggcatgtcct
     3481 cctaaaggag ataaaccaca tcattaccag tttaaagtat gggctctaaa aactgaaaag
     3541 attcctgtag attctaactc cagcggagcg ttagttggtt atatgcttaa tgctaataaa
     3601 atcgcaaccg ctgaaataac accagtttat gagataaagt agggtgagag tatgctggca
     3661 agaggtaaga ctaacttaaa gatcgaagaa atacggatgc ataaacatca tgagattcat
     3721 agggttaagc ctcttatgcc agctttgtgt cgtatccgtc agggaaagaa agttatcaat
     3781 tgggagacgc atactttaac tgttgataat aatcaaataa tattatttcc ttgtggttat
     3841 gaattttata ttgagaatta tcctgaagca gggctttatc ttgcagaaat gctttactta
     3901 cccattgatt taattgagag tttccaaaaa ctttatacgg taactgatca aatacgtaac
     3961 aaaacaagtt tctttttacc tcagaatcct gagttaatat attgttggga gcaactaaaa
     4021 acatctgttt cccgaggctt ctcaactaaa attcaggagc acttagcaat gggcgttcta
     4081 ctttcgttag gagtgaatca tgttaatcat ttacttttat catatagtaa acaatcattg
     4141 ataagtcgtt gttataacct gctgctatcc gaacccggca caaaatggac agcaaacaag
     4201 gttgctcgat atctctacat ttctgtttct acattacatc gccgtctagc aagcgagggg
     4261 gtaagtttcc aaagtatact ggacgatgtg aggttaaata atgcgttgtc tgctatacaa
     4321 acgacggtaa aacctataag cgagattgcc agagaaaatg gttataagtg tccttctcgt
     4381 tttactgaaa gatttcataa tcgttttaat ataacaccaa gagagataag aaaagcttcc
     4441 agagagtaaa agtgttttaa gaaggagcaa ttctatcgat tttgattttg ggaaatcaac
     4501 acggcataat tatgtcaccg gagcctgaac aactccggtg acttctgcgc taaacgggga
     4561 cgtttatgcg cacatacaat ccaaactctc ttctcccttc acagatgcag aaatgcacct
     4621 gcaattcttt gcatctagcg tttgacctct gcggaggtga agcgtgaacc tctcacaaga
     4681 cggcatcaaa ttacatcgcg gcaacttcac cgctatcggt cggcagatcc agccttatct
     4741 ggaggagggc aaatgctttc gcatggtgct taaaccgtgg cgtgagaaac gcagtctttc
     4801 ccagaatgca ctcagccaca tgtggtacag cgaaatcagt gaatacctca tcagcagggg
     4861 taaaacgttc gccactccag cttgggtaaa agatgctctc aaactcacat atctcggtta
     4921 tgaaaccaaa gacctggttg atgtcgtaac cggtgatatc accactatcc agtcgttacg
     4981 ccatacctcc gatcttgata ccggagagat gtatgtcttc ctgtgtaagg ttgaagcctg
     5041 ggcggtgaat attggctgcc acctgactat tccgcagagc tgcgagttcc agctgct
//
LOCUS       DAHBHC010000176         5037 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHBHC010000176 DAHBHC010000000
VERSION     DAHBHC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.180"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..260)
                     /locus_tag="M2A35_004989"
     CDS             complement(<1..260)
                     /locus_tag="M2A35_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCB7201166.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VVVQPAIKGYEPDSNGINQSTTLPLLVDVPVVFPRGGGCTLT"
     gene            complement(320..559)
                     /locus_tag="M2A35_004990"
     CDS             complement(320..559)
                     /locus_tag="M2A35_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HCB7201167.1"
                     /translation="MFGFDKLITPKIINVLYGITMLLLVVAAIITFVNGKAAGALVLL
                     LCAVFCRIFFECIMVSFKNNEYLRRIAEALEANKQ"
     gene            complement(699..1034)
                     /locus_tag="M2A35_004991"
     CDS             complement(699..1034)
                     /locus_tag="M2A35_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201168.1"
                     /translation="MKKVFFCLFLALPLTVSAATQCGPFRFDAGNDGLMHINGQKPET
                     QKMTFLKQKDDFDNVMMQWMLPDPNTGRWLGMDYIKRNKKAILNVEVIRKNMDEPREF
                     WTYDCRKVK"
     gene            complement(1031..2065)
                     /locus_tag="M2A35_004992"
     CDS             complement(1031..2065)
                     /locus_tag="M2A35_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201169.1"
                     /translation="MSKNWMRHFELLLVDDKGDGIKISELKVTFNIQKMPATIFNGFV
                     GNFKVYNLSPTTQNRIMQKEFSRIQVIAGYKGQPDAAGNYPDENVGMIFNGDIRFTVT
                     GKDNATDSWIMLQCIDSWEGHLNASVKTTVAAGWKYRDLFSLGMKSFEPYGIESGAVS
                     DMPETVFPRGRVVYQNTSRLMNHIAGQCKANWWYENNLVNIVPEDKYIGVATVLNANT
                     GLIGMPQQTMGAGVNVRCLINPNIKLGGLIRLDQASVYRASLSNDQVAKSPARLDESE
                     SDGNLYVNGLPGMSQPASINTDGDYIVGSIDYTGDTRGQAWYMDLLCLAKGGKELLAK
                     GTLDKVGDVE"
     gene            complement(2068..2370)
                     /locus_tag="M2A35_004993"
     CDS             complement(2068..2370)
                     /locus_tag="M2A35_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201170.1"
                     /translation="MKAVEIPLVADNQTFATTINGSVYHLSVIWRGEYWVLDLADSNG
                     SAIISGIPMITGADLLAQYRYMDLGFSLVVLCDVAGQENPTQFDLGTLSHLYVFTE"
     gene            complement(2374..3024)
                     /locus_tag="M2A35_004994"
     CDS             complement(2374..3024)
                     /locus_tag="M2A35_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201171.1"
                     /translation="MDFLSVLLQQRTRSIGIIIPDVVITEKHTDALEITEHPVEQPTN
                     AGASGEDAGYISEHAFRRPSEVVMETGFSGGGSLLDFASNLTATSLLGLSPKELYQEL
                     LNLQRNRIPFDVTTGKRIYNNMLIKTLEVTTDKSSENVLLATLTLREVIITSTQSVRV
                     APKNNMTEGVGTSAVQNTGTKTTVPPNNSILKSLPQMAQEGISTVDGYLSNLFLGR"
     gene            complement(3024..5030)
                     /locus_tag="M2A35_004995"
     CDS             complement(3024..5030)
                     /locus_tag="M2A35_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase catalytic"
                     /protein_id="HCB7201172.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSSMPYYRANQYAQMLG
                     IDENTLMAMRRGVGQFSAQYSEMVKAIGFNADQAALSSNRFMTSLKSLGEMAGMARDK
                     IGSNLADGLAGQIDNLRKKIIENFPKIEVTITKVIKGILWLGEIVGRVAFRIVDGVGD
                     IIEWWGKLDAETKTLIEVIGGLVVAMRILNSTFWMSPIGLITGLIVALGLLWEDYKTW
                     KEGGNSLIDWEKWQPAIDKAKDAITWFRDKLLELKDSVGGWQKSLEILGTFIAGVWIS
                     KVLGAFGKISGLPIPPWLKLWALYAGYIVSDRENIADSAKSSLRYTKRIIGDTLAAIG
                     IKTDIGRRDVSEVREWPAWMDWLHGGPGKVIRQGQSNGVVHGSNVQPDIPGGGTLADR
                     NNNPGNIRPVSGKGFRFFESALEGWEAMKNQLMRYFTGKTTGRALQTIQDIVSTWAPA
                     GDNNDPKKYAQDVAKWMGVSPNAILNLTDPQTMGALMQSMARKEGYSNWNSPLAYQAA
                     AGSLNQQTVINVHGVNNPQEAANLIADKQGAVNARAVQQLKGPA"
BASE COUNT         1276 a         1348 c         1082 g         1331 t
ORIGIN      
        1 aaagtcaacg tacagcctcc gccgcgcggg aataccactg gtacatccac cagcaggggt
       61 aatgtcgtcg actggttgat tccatttgag tccggctcat agcctttaat cgcaggttga
      121 acaaccactg ttaccgtgtc cggatcaaat gactggacga tgccgggcat agaaacacgc
      181 agcgccgaca tgacagagcc agcaagtctt acatcggcct gttctttgct accaagttga
      241 gcgcttaaag aaacgggcat tcatatgtct ccagaaagta aaaaacccgc cgggtggcgg
      301 gttcattatt gaagtttcat tactgcttgt ttgcttctaa cgcttcagct attcggcgaa
      361 gatactcatt gtttttaaat gaaaccatga tgcactcaaa gaatattcgg caaaatacag
      421 cacataacaa aagcactaaa gcgccagcag ccttcccatt aacaaacgtt ataatggcgg
      481 caacaaccag aagtaacatt gtgatgccat acagaacgtt gatgattttt ggagttatta
      541 atttatcaaa tccgaacatg tgacaaattc cttatcgtga aagtagaagt atcacattat
      601 aattacgagt gattaatcaa caatcttttt gcatggaaag gaaccgatga ttttcggtgc
      661 atccatgctg ttctgcagaa gctggacgcc aatgtaattc acttcacctt ccggcaatca
      721 tacgtccaga actcccgagg ctcgtccata tttttgcgga taacttcaac gttgaggatc
      781 gcttttttgt ttcgtttgat gtagtccata cctaaccaac gtccagtatt aggatcaggt
      841 aacatccatt gcatcatgac attatcgaaa tcgtcttttt gtttcaaaaa ggtcattttt
      901 tgtgtttctg gcttttgacc attgatgtgc atgaggccat cattaccagc atcaaagcgg
      961 aatggtccgc actgcgttgc cgccgaaact gttaacggca gggccaagaa taaacaaaaa
     1021 aatacttttt tcactctaca tctccgactt tgtccaaagt gccttttgcc aatagttctt
     1081 tgccaccttt agccaggcaa agcaggtcca tataccacgc ctgccctcgg gtgtcgccag
     1141 tataatcaat gctgcccaca atgtaatcac cgtcagtatt aatgctggca ggctgtgaca
     1201 tgcctggcag accgttaacg tagagattac cgtcgctttc agactcatcc agtcgtgctg
     1261 gcgatttcgc tacctggtca ttactcaaag aggcacggta tacagatgcc tgatccagac
     1321 gaataagccc accgagctta atatttggat taatcagaca tctgacattt acgccagctc
     1381 ccatcgtctg ctgtggcata ccgataagcc cggtgttagc attcaacacc gtagcaacac
     1441 caatatattt atcttcagga acaatattta ccagattatt ttcataccac cagttagcct
     1501 tacactgccc tgcgatatga ttcatcaacc ttgatgtgtt ttgataaaca acgcgacccc
     1561 ggggaaatac cgtttcaggc atgtcagaaa ctgcgccgga ttcgatgcca tatggttcga
     1621 atgatttcat acccagactg aaaagatctc tgtacttcca gccagctgcc actgtggttt
     1681 tcacacttgc gttcaggtgg ccttcccagc tgtcaataca ctgcaacatg atccaactgt
     1741 ctgtggcatt atctttacca gtgacagtaa aacggatatc tccattgaat atcataccaa
     1801 cgttttcatc aggataatta cctgctgcat ccggttgccc cttgtatccg gcaataacct
     1861 gtatacgcga aaactccttc tgcataatcc ggttctgagt ggtaggagac aggttataaa
     1921 ccttaaaatt tccaacgaat ccattaaata tggtcgcagg cattttctga atattgaaag
     1981 tgactttaag ctcagaaatt tttatcccgt cgcccttatc atcaacaagc aataattcaa
     2041 agtgacgcat ccagtttttc gacattgtta ctccgtgaaa acatagaggt gtgagagcgt
     2101 tccaagatcg aattgcgtcg gattctcctg ccctgccacg tcgcagagca ccaccagaga
     2161 aaaacccaga tccatatatc gatactgtgc cagcaggtca gcccccgtaa tcatcggtat
     2221 acctgatata atggcggagc cattgctgtc agcaagatcc agaacccagt actcgcctcg
     2281 ccagatgaca gacaggtgat aaaccgaacc gttaattgtg gtggcaaaag tctgattgtc
     2341 agcaaccagt gggatttcta cggctttcat gaatcacctt cccagaaata aattgctcaa
     2401 atacccatca acagtggaga taccttcttg cgccatctgt ggcagcgatt tcagaatgga
     2461 attattcggc ggcaccgttg ttttggtgcc tgtattctgc acagcagacg ttccgactcc
     2521 ctcggtcata ttgtttttcg gggctaccct gactgactgc gtggaggtaa taattacttc
     2581 cctgagggta agtgtcgcca gaagcacatt ttcactactc ttatcggtcg tgacctccag
     2641 cgtttttatc aacatattgt tgtaaatacg cttgccggtt gtcacatcga aaggaatacg
     2701 attccgctgc aggttaagca gttcctgata cagttctttc gggctcagcc ccagtaaact
     2761 ggtagccgtc aggttactgg caaaatcaag cagcgatccg cctccggaaa aaccggtttc
     2821 catcacaacc tcagaagggc gcctgaatgc gtgttctgat atgtaaccag cgtcctcacc
     2881 actggcacca gcattcgtgg gctgttcaac cggatgttcc gtaatttcca gggcgtcagt
     2941 gtgcttttcg gtaataacca catcaggaat aatgattcct attgagcggg ttcgctgctg
     3001 cagtaaaaca gataaaaagt ccattacgca ggtcctttca attgctgtac cgccctggca
     3061 tttacagccc cctgcttgtc agcgatcaga ttagccgcct cctgagggtt gttaactcca
     3121 tgaacattta taacagtctg ctggttaagg ctgccagcgg cggcctgata cgccagcggg
     3181 ctgttccagt ttgaataacc ttctttgcgc gccatcgact gcatcaatgc tcccatagtc
     3241 tggggatctg taagattcaa tattgcattc ggtgatactc ccatccattt cgcaacatcc
     3301 tgtgcatact ttttcggatc gttgttatca cctgccgggg cccaggtact gacaatatcc
     3361 tgaatagtct gtaatgcccg tccggttgtt ttcccggtaa agtaacgcat gagctggttt
     3421 ttcatcgcct cccagccctc aagcgctgat tcgaaaaacc ggaacccttt accgctcacc
     3481 gggcgaatgt tcccggggtt attgttacga tcagcaagag tgccgccgcc ggggatgtcg
     3541 ggctggacgt tggagccatg cactacaccg ttactctgtc cctgccggat aactttaccg
     3601 ggtcccccgt gcaaccagtc catccacgca ggccattcac ggacctcact gacatccctg
     3661 cgtccgatat ctgtttttat accaatagca gccagcgtgt cgccgataat tcgcttcgta
     3721 tacctcagtg aagattttgc gctatcggca atattttctc tgtcactaac aatataaccc
     3781 gcgtaaagcg cccacaattt aagccagggt gggatcggca aacctgatat ttttccgaaa
     3841 gcccccagaa ccttggatat ccagacaccc gcgatgaatg taccgaggat ttccaatgat
     3901 ttttgccatc cccccacgct gtctttcagc tcgagtaatt tgtcgcggaa ccaggtgatc
     3961 gcatccttcg ctttatctat tgccggttgc catttttccc agtcgataag gctgttaccg
     4021 ccttctttcc atgttttgta gtcttcccac aagagaccga gagccacgat cagaccggta
     4081 atcagcccta taggtgacat ccagaaagta gagttaagta tccgcatggc gacaaccaga
     4141 ccgccgataa cctctatcag ggttttcgtt tcggcatcca gtttccccca ccactcgatg
     4201 atatctccga caccatcgac tatccgaaat gctacccgcc cgactatctc acccagccag
     4261 aggatcccct ttatgacctt tgtgatggtg acttcaattt tgggaaaatt ttcaattatc
     4321 tttttgcgca ggttatcaat ctgccccgcc agtccgtccg caagattcga tccgattttg
     4381 tcccgcgcca tcccggccat ttcaccgagc gatttcagcg aggtcataaa ccggtttgac
     4441 gataaggcag cctgatcggc attaaatccg atcgctttca ccatttctga atactgagcg
     4501 ctgaactgcc ccactccgcg acgcatagcc atcagggtat tttcgtcaat gcccagcatc
     4561 tgcgcatact ggttagcccg gtaatacggc atgctgctga gtttctgtcc aacgcccgta
     4621 aaaatagcgg ccatgtcacg catgttaccg ctggcatcac gggtctgtac gcccaggcga
     4681 ttcagaaagc cttctgcacc gggattgtta cgaataaacc gggagaggct ttccagagaa
     4741 gatcgcgcag cgtccacact gccgccaacc tgcgaaaccg catagccaat agactgaatt
     4801 ccctggaccg tcgcgccggt gcgctgtgac gcccagtaaa gattatccag gccggaggcg
     4861 atcttagccg tgaaggccac cacggacagc gcagcccctt caacagccag ccccattttg
     4921 atgacatttg cagttgtacc ggcgaggaca gaaccgaact ttttcgctcc tgcatcatcc
     4981 acactgaagc caagcgagac gaggaaatct ttaatagttt cagcgttcat tatcctc
//
LOCUS       DAHBHC010000177         5025 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHBHC010000177 DAHBHC010000000
VERSION     DAHBHC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.181"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..629)
                     /locus_tag="M2A35_004996"
     CDS             complement(<1..629)
                     /locus_tag="M2A35_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201173.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRRTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGNTKDTNTKENRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAP"
     gene            complement(662..955)
                     /locus_tag="M2A35_004997"
     CDS             complement(662..955)
                     /locus_tag="M2A35_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCB7201174.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(1075..1290)
                     /locus_tag="M2A35_004998"
     CDS             complement(1075..1290)
                     /locus_tag="M2A35_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201175.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            1394..2026
                     /locus_tag="M2A35_004999"
     CDS             1394..2026
                     /locus_tag="M2A35_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCB7201176.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            2023..2427
                     /locus_tag="M2A35_005000"
     CDS             2023..2427
                     /locus_tag="M2A35_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201177.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGAMNGDRYEFTKDST
                     EDSVFNVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            2647..3102
                     /locus_tag="M2A35_005001"
     CDS             2647..3102
                     /locus_tag="M2A35_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCB7201178.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            3111..3980
                     /locus_tag="M2A35_005002"
     CDS             3111..3980
                     /locus_tag="M2A35_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201179.1"
                     /translation="MKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNAHGDG
                     DKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGDLIDR
                     SVDKDPDTNSFLLTNKEYLYGKFGINGWQKYVRFLRAFVDAYKNSDMIYTYLPGGDNC
                     TLSADNRIFIPVLGLGLINAVNEKDVIIVKQWRKYEGYRYLPCFDILKIQNKFYVKIK
                     YKEDIFFLRKNIDLLQELSGTIDVILGSRFIKELKESRSFSRVEISESELWGISNNPV
                     NHAHQRNPNKKWS"
     gene            4169..4537
                     /locus_tag="M2A35_005003"
     CDS             4169..4537
                     /locus_tag="M2A35_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HCB7201180.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            4610..4774
                     /locus_tag="M2A35_005004"
     CDS             4610..4774
                     /locus_tag="M2A35_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCB7201181.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            4743..4886
                     /gene="kil"
                     /locus_tag="M2A35_005005"
     CDS             4743..4886
                     /gene="kil"
                     /locus_tag="M2A35_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCB7201182.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            4961..>5025
                     /locus_tag="M2A35_005006"
     CDS             4961..>5025
                     /locus_tag="M2A35_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCB7201183.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQ"
BASE COUNT         1475 a         1055 c         1088 g         1407 t
ORIGIN      
        1 ggcgcgatag tcttcaccat gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc
       61 ccccatttgc tgccgctctg aattgcagca tcaggtttca ccacagaaag gtcgttttct
      121 ggctggtcag aggattcgcc agaattctct gacgaataat cttttctatt ttcttttgta
      181 ttagtgtctt ttgtgttccc ctgttttgag ggatagcaat cccccaattt gagggatgtt
      241 ttatccctcg ttttagggga ttttccctcg ttttgaggga tacaccattc tgagatgttt
      301 ttatttggtc caaacatgcc gccttgctgc ttgataatat tcattctgac gagttctaac
      361 ttggcttcat tgcaccgttt gacaggtaac tttgtaatct cgctaagttg agaatcggtg
      421 attctgtcca ttggtttatt ccacccatag gttctacgca gaatggcaag cagcacttta
      481 aactgtcgct tggtcagatc tgcgcctgaa taagcctcaa gcagcatatt tgatagtctg
      541 gcgtaaccat catcgagatc tgccacatta cgctcctgtc cggcaaagtt acctctgccg
      601 aagttgagta tttttgctgt atttgtcata atgactcctg ttgatagttc cagtaataac
      661 ctcagaattc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
      721 tgagaatcga agcaacttgt cgtgccaatc gagccatgtc gtcgtcgacg acaccccatt
      781 caagaacagc aagcagcatt gagaactttg gaatccaatc cctcttccac ctgctgatct
      841 gcgacttatc aactcccaca gcttccgctg tcttctcagt tccaagcatt gcgattttgt
      901 taagcaacgc actctcgatt cgtagagcct cgttgcgttt gtttgcacga accatatgta
      961 agtatttcct tagataacaa ttgattgaat gtatgcaaat aaatgcatac accataggtg
     1021 tggtttattt tgatgccctt tttcagggct gggatgtgta agagcgggaa tgtcttaagc
     1081 ggctttaccg cgtttagttc cgtactgtaa ccaaaccgga tcacagttaa gcgccatagc
     1141 aatctcaaac aagaagcgcg gtcgcttggt tactccagct tcaatcagtt gaattgattg
     1201 ctgtttaaca ccggctttgg ttgccagttc ggtttgcgtc atttttaacg caattcgcct
     1261 cttcttgagg cgttcagaaa gagtttgcat atcgcctcca tcaacaaact ttcttgtatt
     1321 ttcatacaat gtatcttgtt tgtcaaatac agtttttctt gtgaagattg ggggtaaata
     1381 acagaggtgg cttatgagta tttcttccag ggtaaaaagc aaaagaattc agcttggact
     1441 taaccaggct gaacttgctc aaaaggtggg gactacccag cagtctatag agcagctcga
     1501 aaacggtaaa actaagcgac cacgcttttt accagaactt gcgtcagctc ttggcgtaag
     1561 tgttgactgg ctgctcaatg gcacctctga ttcgaatgtt agatttgttg ggcacgttga
     1621 gcccaaaggg aaatatccat tgattagcat ggttagagct ggttcgtggt gtgaagcttg
     1681 tgaaccctac gatatcaagg acattgatga atggtatgac agtgacgtta acttattagg
     1741 caatggattc tggctgaagg ttgaaggtga ttccatgacc tcacctgtag gtcaaagcat
     1801 ccctgaaggt catatggtgt tagtagatac tggacgcgag ccagtgaatg gaagccttgt
     1861 tgtagccaaa ctgactgacg cgaacgaagc aacattcaag aaactggtca tagatggcgg
     1921 tcagaagtac ctgaaaggcc tgaatccttc atggcctatg actcctatca acgggaactg
     1981 caagattatc ggtgttgtcg tggaagcgag ggtaaaattc gtatgatcag gattgcggcg
     2041 ctactctcaa tactcttaac taccagcgcc aattctgaat gctggattgt cacaaacctg
     2101 cacgggtacg gggcaatgaa tggcgatcgt tacgagttta caaaagacag cacggaagat
     2161 tccgttttca acgtaacaat aaatggcgat aaatcatcag tttatgaatc agtttctggc
     2221 gtctatccag agatgaaata cactgctttg tcatcgaaca ctatggtagg agaataccag
     2281 tctggaggag gcataaccgt tgaaacttgg tcaatcacta cagacaaaaa agctctttac
     2341 tccaaagtaa tgaacatccc aggtatgcaa caacttacat caaccaaatc ctttgttggt
     2401 gatgtagtcg gaacctgcaa tcagtaatcc ccacctcaat ctcgataaca aaaaaacaaa
     2461 ctattttccg tttaaaaaca atggagtttg tttttcacgc ccctttttac aatatttctt
     2521 gtttacaaca tacaatcttc cttgtaattt taagccatca gcaggacgca ctgaccacca
     2581 ttgaaggtga ggctcttaaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc
     2641 tttggtgtgt gtgatacgaa acgaagcatt ggccggaagt gcgaatccgg attagctgcc
     2701 aatgtgccat tgcggggtgt tttcgttcag gactacgact cccacacaca accaaagcta
     2761 actgacagga gaatccagat ggatgcacaa acacgccgcc gcgaacgtcg cgcagagaaa
     2821 caggctcaat ggaaagcagc aaatcccctg ttggttgggg taagcgctaa accagttaac
     2881 cgccctattc tctcgctgaa tcgcaaaccg aaatcacgag tagaaagcgc actgaatccg
     2941 atagacctta cggtgctggc tgaataccac gaacagattg aaagcaacct gcaacgtatt
     3001 gagcgcaaga atcatcgagt ttggtatagc aagccaggtg agttcggtat aacttgtcaa
     3061 ggaagacaaa aggttaaagg ggaatccatt ccattggcat gaggtacgta atgaagaaga
     3121 ttgattacaa gtccatacca aaaccaatag actcagcatc agagcgaaaa aaacacaaaa
     3181 aagaggctga aaaattagca aattatatca gttttattag aaacaatgct cacggcgatg
     3241 gcgacaagaa gttgctttca gatgctcgga cccaagcgtt cggtatactt cgtaagcaga
     3301 tgcaatatcg tcttcatcct ggctacataa ttgaaattcg ccctactgaa agacaattgt
     3361 tcttattaaa ctctgtcttt gactttgtaa acgtagttgg agatcttatc gacaggtctg
     3421 tagataagga tcctgataca aatagttttc ttctaacaaa taaagaatac ttatatggta
     3481 aattcggtat aaacggatgg caaaaatatg tacgcttctt acgtgcattc gttgatgcgt
     3541 ataaaaattc cgacatgatt tatacatatt tgcctggtgg tgataattgt acgctttcgg
     3601 cagacaatag aatattcata cctgtacttg gtcttgggct gattaatgca gtaaatgaaa
     3661 aagatgttat aattgttaag cagtggcgaa aatatgaggg atatagatat ctgccatgct
     3721 ttgatatatt aaaaatacag aataaattct atgtaaaaat taaatataaa gaagatattt
     3781 tcttcctcag aaaaaacata gatcttttac aagaactttc tggtacaata gatgtcatct
     3841 taggttctag gtttataaaa gaactgaaag aaagcaggtc tttctctcgt gtagagataa
     3901 gcgagtcaga gttatggggt atatcaaata atcctgtaaa ccacgctcac caacgaaacc
     3961 caaacaaaaa gtggtcataa cccgctcagg cggatttcat tttcacgcaa acaacagaat
     4021 aaacacagcg ctgtgtattc attccaacga gtgaatacac ggagcaatgt cgctcgtaac
     4081 taaacaggag ccgacttgtt ctgattattg gaaatcttct ttgccctcca agatgagggc
     4141 gattttttat ctgtgaggat atgaacagat gtcaaacatc aaaaaataca tcattgatta
     4201 cgactggaaa gcatcaatag aaattgaaat cgaccatgac gtaatgacag aggaaaaact
     4261 tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac acggctctgt
     4321 attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa tttcaagcga
     4381 cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg gtcaggaagg
     4441 atggcctcca atggatggta gtgaaggaat aagaattacc gatatcgata catcaggaat
     4501 atttgattca gatgatatga ctatcaaggc cgcctgagcg cggcgttacc gcataccaat
     4561 tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca tgcaatatgc
     4621 cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac gaatcacccg
     4681 taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag gagtcccaaa
     4741 aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact tttattggcg
     4801 atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta atactgaaac
     4861 tgagatcaag caaaagcatt cactaacccc ctttcctgtt ttcctaatca gcccggcatt
     4921 tcgcgggcga tattttcaca gctatttcag gagttcggcc atgaacgctt attacattca
     4981 ggatcgtctt gaggctcaga gctgggcgcg tcactaccag cagat
//
LOCUS       DAHBHC010000178         5016 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHBHC010000178 DAHBHC010000000
VERSION     DAHBHC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.182"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..990)
                     /locus_tag="M2A35_005007"
     CDS             complement(<1..990)
                     /locus_tag="M2A35_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCB7201184.1"
                     /translation="MKYGSVCSGIEAASVAWESLGWQPAWFAEIEPFPAAVLAHHWPQ
                     VKNLGDMTKIAAGVLADQIEAPDVLVGGTPCQAFSVAGLRNGLADERGQLTLSYVELA
                     NAIDDKRRQRGERESIIVWENVPGVLNSKDNAFGCFLAGLAGESSALQPPGGKWTLAG
                     CVRGPARNIAWRVLDAQFFGVAQRRRRVFVIASARDDIDPAKILFESDGVRRDTPPRR
                     ESQTAVAALTAHGVGACGADDNQAQAGHLIPEISAYRMTAFGEYADDETASTVKARDY
                     KDATDLAVMTVHGTQDPDVNIELAHTLGRNNGQENACIAFSYKDHGQDAGYEIA"
     gene            complement(987..1346)
                     /locus_tag="M2A35_005008"
     CDS             complement(987..1346)
                     /locus_tag="M2A35_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7201185.1"
                     /translation="MAKYSITPLGKPRMTRADKWKQRPPVLRYRVFCDEVRLSGITLP
                     ENGAHVVFVMPMPASWSQKKRASMNGAAHQQKPDIDNLTKSLLDALFDDDSHIWDVRA
                     SKIWGYEGAIVVQEGAA"
     gene            complement(1346..1636)
                     /locus_tag="M2A35_005009"
     CDS             complement(1346..1636)
                     /locus_tag="M2A35_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7201186.1"
                     /translation="MTKFDRIWLSRVASLGCIVCKNLGYGESPAEIHHVRTGQGTAQR
                     AGHQQTIPLCCQHHRVGGYGVAIHSGKKTWEKKYGQEIDLLNQTLTALGENV"
     gene            complement(1737..1910)
                     /locus_tag="M2A35_005010"
     CDS             complement(1737..1910)
                     /locus_tag="M2A35_005010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201187.1"
                     /translation="MNERELLDIVRPDGRYELISQGGKFIVRPLRADEIIISPESHRE
                     CKKRFSNTQTELQ"
     gene            complement(2067..2711)
                     /locus_tag="M2A35_005011"
     CDS             complement(2067..2711)
                     /locus_tag="M2A35_005011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201188.1"
                     /translation="MKDLYNDEWSAGFIARVDLLRELDNQSITQPLYRERADMIFTEM
                     TARFTARLRETLGDIDDPGVTLDDAIRYYVNGERGDPLLDYLSSRTRPFCDAMHISSD
                     AYGIAAYCCAFMMWKGDLRVAYAFFSLLMRPLVSAYRYRIDAQRRGSAGGHPHHRLRP
                     EALELAAEYFTENPRATNADAAKHILNVLNVKYSDPPHAISVARWLKTIYGDNK"
     gene            complement(2799..3380)
                     /locus_tag="M2A35_005012"
     CDS             complement(2799..3380)
                     /locus_tag="M2A35_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCB7201189.1"
                     /translation="MTTQITISDIAIHQDNDGRYSINDLHKASGAEHRHLPNYWLELQ
                     QTKELIEEILNTGIPVITPPVVSRKGRNGGTYVCKELVYAYAMWISAAFSLRVIRAYD
                     ALVAENSQNNAITAIPTSPHSYSIVTSFCDGEPIYSRVIQPGEILIHRDDHIELMARA
                     GYMTIHCNELEKMGAPELARRIEATRRMMDIWA"
     gene            complement(3482..3649)
                     /locus_tag="M2A35_005013"
     CDS             complement(3482..3649)
                     /locus_tag="M2A35_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCB7201190.1"
                     /translation="MTIITHRGYIHRTPAKSGVGIGLPDIYSTHTALSGFFMRKARLH
                     PRVMVGCVRAS"
     gene            complement(3646..4234)
                     /locus_tag="M2A35_005014"
                     /pseudo
     CDS             complement(3646..4234)
                     /locus_tag="M2A35_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(4469..4642)
                     /locus_tag="M2A35_005015"
                     /pseudo
     CDS             complement(4469..4642)
                     /locus_tag="M2A35_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT         1124 a         1325 c         1235 g         1332 t
ORIGIN      
        1 cgctatttca tatcctgcat cttggccgtg atctttatag ctgaatgcta tgcaggcatt
       61 ttcctgccca ttattccgcc ccagcgtgtg cgctaattcg atgttaacgt ctggatcctg
      121 tgtgccatgg accgtcataa ctgccagatc ggtcgcatct ttgtaatctc tcgctttaac
      181 tgtcgatgca gtttcatcat cagcatactc gccgaatgcg gtcatgcgat atgcgctgat
      241 ctcagggatt agatgtcctg cctgcgcctg gttgtcgtct gcgccacacg ctccaacgcc
      301 gtgcgcagta agagcggcaa ctgccgtttg cgactctctg cgcggcggag tatcccggcg
      361 cacgccgtcg gactcaaaaa gtatttttgc gggatcgata tcgtctctag cacttgcgat
      421 aacgaacaca cggcggcgtc gttgtgccac tccgaaaaat tgagcgtcga gcactcgcca
      481 ggcaatattt cgcgcgggtc cacgcacaca acctgcgagc gtccattttc cccctggtgg
      541 ctgcaacgca ctgctttctc cggcaagccc agccagaaaa catccgaacg cgttgtcttt
      601 gctgttgagc acgccgggga cattttccca gacgatgatt gattcccgtt caccgcgctg
      661 gcggcgcttg tcatcgattg catttgctaa ttccacgtaa gaaagggtca attgtccgcg
      721 ttcgtcggcc agtccattgc gcaatccggc aacgctgaat gcctggcagg gagtgccgcc
      781 caccagcacg tctggtgcct cgatttgatc agcgaggaca ccggcggcga ttttcgtcat
      841 atcgccaagg tttttgacct gcggccagtg atgcgccagc acagcggcgg ggaacggctc
      901 aatctctgcg aaccatgcag gctgccagcc gagggactcc cacgccacac tcgcggcctc
      961 aataccgctg caaacggaac cgtatttcat gccgcacctt cctgcaccac gatcgcgccc
     1021 tcatagcccc atattttcga cgcccggaca tcccaaatat gagaatcgtc atcaaacagg
     1081 gcgtctaaca gcgatttagt gaggttatca atatccggtt tttgctggtg ggccgcaccg
     1141 ttcatgctgg cgcgtttttt ctggctccag ctcgctggca taggcataac aaatacaacg
     1201 tgtgcgccgt tctcgggcag cgtaatgccg cttaagcgga cctcatcaca aaacacgcgg
     1261 taacgcagca ccggcgggcg ctgtttccat ttgtcagcac gggtcatgcg gggtttaccc
     1321 agtggggtga ttgaatattt tgccatcaga cgttttcccc cagcgcggtt aacgtctgat
     1381 tcagcagatc aatttcctga ccgtattttt tctcccaggt ctttttgcct gagtgaattg
     1441 ctacgccgta accaccaaca cggtggtgct gacagcacag ggggattgtt tgctggtggc
     1501 ctgcacgctg ggcagtgccc tgaccggtgc gaacgtggtg aatctccgcc gggctttcgc
     1561 cgtagccaag atttttacag acaatacagc cgagcgacgc aacgcggctc agccagatac
     1621 gatcgaattt agtcactgaa tacccccggg gcagtcagcg acgctttaat gcagatattg
     1681 tgcgccattt tgatttcctc aatttggcgc gacgggtcgg tgttcagccg agggggttat
     1741 tgtaattcag tttgcgtatt actgaaacgt tttttgcact ctctgtgtga ttcgggggaa
     1801 ataataattt cgtcagcgcg taacggcctc acaataaatt taccgccctg gctaatcagc
     1861 tcataacgcc cgtcaggccg gacaatatct aataattcgc gttcgttcac gttatccccc
     1921 gtcatacaag atactgtaca aatatacagt atcattttat ggcattgctg tctcaaataa
     1981 ttaatctggg gaaataatgc tctaatatat tcacgcgaca aatatacaca accagcttgt
     2041 gtagtacata agctggttgt gtttcgttat ttattatctc cgtaaatggt ttttaaccac
     2101 cgcgcaaccg atatagcatg aggagggtcg gaatatttaa catttaatac atttaaaata
     2161 tgctttgcgg cgtccgcatt tgttgccctc ggattttctg tgaaatattc cgccgccagc
     2221 tccagcgcct ccgggcgcag ccggtggtgc gggtgtccac cagccgagcc acggcgctgc
     2281 gcgtcgattc ggtatcgata cgcagatacc agggggcgca ttaacagaga gaaaaacgca
     2341 tatgccaccc tcagatcacc cttccacatc atgaacgcgc agcaatatgc cgcaattccg
     2401 taggcatcgc tggaaatgtg catcgcgtca caaaatgggc gagtgcggga ggacaggtaa
     2461 tctaacagcg ggtcgccgcg ttcgccgttc acgtaatagc ggattgcgtc gtccagggtg
     2521 acgcccggat catcaatatc acccaacgtt tcccgcagtc gtgcagtgaa tcgcgccgtc
     2581 atttcagtaa aaatcatgtc ggcgcgctct ctgtagagag gctgtgtgat tgactgattg
     2641 tccagctcgc gcagcaggtc aacccgggcg ataaacccgg cggaccactc atcattgtat
     2701 aagtctttca tagctacctc gaatagttaa cgcaaaatat atttgcatta ataattattc
     2761 gaggtttgtt ataactttga aacactatcc gggcggtatt acgcccagat atccatcatt
     2821 ctgcgcgttg cctcgatgcg gcgcgccagc tcaggtgcgc ccattttttc cagctcattg
     2881 cagtggatcg tcatgtagcc tgcgcgggcc atcagttcga tatggtcatc gcgatgaatc
     2941 aaaatttcgc caggttgaat aacacgactg taaatcggct cgccgtcgca aaatgaggta
     3001 acgatcgagt agctgtgtgg gctggtgggg atggcggtaa ttgcgttatt ttggctgttt
     3061 tccgccacca gcgcatcgta ggcacggatt acacggagag agaacgcagc gctgatccac
     3121 attgcgtagg cgtaaaccag ttctttgcag acgtaggtgc cgccgttacg gccctttctg
     3181 cttaccactg gtggagtaat taccggaatt ccggtattta agatctcttc gattaactct
     3241 ttggtttgct gcaattccag ccagtagtta ggcaggtggc gatgttcagc gccggatgct
     3301 ttgtgcaggt cattaataga ataacgtccg tcgttatcct gatgaatggc gatatcagaa
     3361 atagtgattt gagtagtcat agcgtcacct cggtgggtta accatcacca ccgtcgagac
     3421 caatcgactg gtggtgaact gtgcaaggtt ggtcttaccg gctactcaaa cccggcgctt
     3481 cctaagaagc cctcacacag cccaccataa ctcgcgggtg tagccgtgct ttacgcataa
     3541 aaaaaccgct taacgcggta tgcgttgagt agatatccgg gagaccaatc ccgacgccag
     3601 attttgctgg cgttcggtga atatagcccc ggtgggtgat tattgtcaat tgtcctgatg
     3661 tggcgctgtc tctgcaatca ggcgctccag ttcagcgcgg gcctctgcca gctcaatggc
     3721 gtgatagcag ccgcgactgc cgtcactatc taatagtcta tttacagcgg tgatccaatc
     3781 gtccggagtt accggagagt tgccaccagg aatattttct ggaatatttt gttgtgcgtt
     3841 ttgtggttgt tcggatttac cctgaagcgt ggctgcgcgg caggcgttcc acgccttctc
     3901 atacgcttca gcaggggtta gccgcctgga ttccaatatc gcctgttcaa acgtgataac
     3961 tggcactacc ggcgctggcg gggcggcgta gacttcaata accccattat aaataggcca
     4021 ttccccatcc ttgacgtagt cacttgtgcc gtcaacttgc tgctctgtaa tgtggaaagc
     4081 gcctattggt tttgcttcca gcgaggcgag tgcctttttt taacgcaatt agcgtattac
     4141 tttcatcttc attgaggccg aacggcattt catcgcgtaa cgcctcaatg cccgcaattg
     4201 tggtttgcaa ccactcttta tttaaaatat tcatatgcca cctttaagca ccataccatt
     4261 tcacatatga atatggcgcg ttatttaaat cgagatcctt gccaattttg ccggacatat
     4321 cacactcagg acaataaact ggagaaccac ggcgcggatt gtttgaatga ctatatattt
     4381 cctcaacgag taatattgaa ctcccacagt taggacacga gccgatttta tttgttatga
     4441 ataaataata atccagtaac cccatgtatt actcaccctc cacagctacg ccagcggcga
     4501 tggctgactc ttcatgtgcg cgtttagcgg cattgagaat tgctgccagt ggggtatagc
     4561 cgccatccat tcggattgta ttgtgaatgg tcgccagcac gtcgcgaagc tttctatggc
     4621 tcgcctcaag ttctgatatc cggttgcgca atttatcgcg agaattagcc acttttatgc
     4681 cgtatctaac tgatgcggag ttcatgcccg ccaggactgt tacgcggcgt ttcgttgctg
     4741 ccagatcatc caccagcgca atcaccgctt ctggcgttgc taaatcctgc cagttgtctg
     4801 acgtgtcgct ggcctggcgg cacataattt cctcttctgc cgccgagcgt aatttttgat
     4861 atttattcat aatatacatt ccctgttatt taatgctgtt tcggatttaa tatcatcaat
     4921 ttgatatgtg attttaattg gctccgggtt tttatttgag cgaataaaac ttggcatccg
     4981 ttcaaattcc atcagatagc aaaccaggcc aaaaac
//
LOCUS       DAHBHC010000179         4963 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHBHC010000179 DAHBHC010000000
VERSION     DAHBHC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.183"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..339)
                     /locus_tag="M2A35_005016"
     CDS             complement(<1..339)
                     /locus_tag="M2A35_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7201191.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRT"
     gene            complement(341..754)
                     /locus_tag="M2A35_005017"
     CDS             complement(341..754)
                     /locus_tag="M2A35_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201192.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1286..2212
                     /locus_tag="M2A35_005018"
     CDS             1286..2212
                     /locus_tag="M2A35_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201193.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            2257..2511
                     /locus_tag="M2A35_005019"
     CDS             2257..2511
                     /locus_tag="M2A35_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201194.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            3010..3354
                     /locus_tag="M2A35_005020"
     CDS             3010..3354
                     /locus_tag="M2A35_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HCB7201195.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKVFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(3530..4531)
                     /locus_tag="M2A35_005021"
     CDS             complement(3530..4531)
                     /locus_tag="M2A35_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HCB7201196.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETRPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
BASE COUNT         1231 a         1314 c         1318 g         1100 t
ORIGIN      
        1 cgttcttcct gccgtcctgg cggctttttc ggtccgttgt ttcagggtcg caagctgcac
       61 aaacggatac ggaggcgtga gcgaaaaatc cccccgcgtc agcgccagtg cttcattaat
      121 gcgcgcaccg gtgttccaca gtgtggccag cagcatcttg cggcgcagat ccgggacgta
      181 atggagcagg gcgctcactt caggggccag caggtatttg ggcagttcat catgaaccat
      241 cgacatctgg cggagtgcca gcgctgccgg ataatcgatg gcaacaggaa gctgagcggc
      301 atcccgggca tggtggatcg gtgccggcag gtgctgcata tcagtgttca ctccacgggg
      361 aaacgtcggg caggttttcc acaacccggt attttcccgg ataacggagc tggttaccgc
      421 cacgcgtcca gacggtgaca acaaaacccc ctccctggtt gttttcgggg agcaggcgat
      481 cgcactgctc actcagcatg tggatcacga acggcgcatc gctgccgtct tcaaatacca
      541 gttccagcgc atcgcggcca tggagcgttc ccttactgat gatgacgtac tcagctcccc
      601 gcatctccct gagtaaatgt tttgccgcat ccgggacaag cagcctggct gcgccggcat
      661 tccagctgag gtagacatat ccggcctgcg cctgtacaga ctgccagtaa tccgttctgg
      721 cgacagcctg accttcattc tcaataaaaa acatgggtga ctcctttttt cttttccggg
      781 cagggacggt catgcgataa cgtcatccat catcactgcc cttaccgtca cgccgtctga
      841 taacatcatg ccgttatcat catcaccatg ataacaagca tccatataac cacgcagtta
      901 aattaccgga gagccgtttc cgtcagatcc gactttccgg aatatcacaa ctttccggaa
      961 gtgtaccacg atgaggaaat aatcatatcg ctgttttccg cccctgcacg gcgcatcaca
     1021 ggcaagggaa ataacacaac atatagtgtc atcagcagct gaatgcagca ctacatattg
     1081 tgcttacagg aggtggcgtt gtgtgggggg agaggaagat ccgtgacctg gggttttcgg
     1141 atctcagaaa aggaagtgaa cagaaaacag acagcgaagc atacgaatta taaaaacgta
     1201 tgcttcgcag tatttaaaga cactcaaaac caggatagag tataagtatt gcagcaatgc
     1261 cccctcacat gaaaaggtac gcgggatgag tgaaaaagaa catgacatga caaacgacgg
     1321 tggagagagc gtaacgtata ccctgcggaa catccccgct gacaccgacc gggtgatcac
     1381 cgacctggca agccacgccc gtaagccaaa ggcgacgttt ctgcgtgagt tcctggaaga
     1441 cagcttccgc gacgtcatcg acagctttgc cctgaagaac ccgctcatcg cctccctgga
     1501 tgaggagctg gccagttatc tggacgctaa ggtcatggag cagcgctacc agagccattt
     1561 tatcacccgc tggaatcagg aataccagaa gctgctgggg atctcgacgg aagaggagct
     1621 ccgccggttg gtactgaaca acaccccctt cctgcaggtc cgtgccgacc aggtgcttaa
     1681 ggggtggaaa aatatcccgc gcgggatctc cctgaccttc tccctgtttg ccgagatcgc
     1741 cggccgggac agggaaacca tcgaccaggc ctggaaaaat atcttctact cgcaactgag
     1801 ggaaaaaaaa caccgctttt accaagatat cgaggccatc cgcgccctga aaaaactgcc
     1861 tgccctcacc ggcgacagct ggacgcggga tggcatcacg gtgcgtatct accgcccgga
     1921 aaattacgcc cgcggcgcat ggcgcgttac actgagcctc ccggaaaatt acgccaccca
     1981 gatgtggaac atcccgttcc cggagcttga ataccgcctc tttaccgccg atccgggcta
     2041 cagcgccctg atcagcgccg aacccgacag gtgggacaag gccttccgtt ttgtggacgg
     2101 ggtgtgtgag cttcaccttt acaccaacgg tgtggaagaa gatcacaatc ccaccccgct
     2161 cggggatgtc gctcaggcgc tgatcaacgt ggtggaagaa aacctgctgt aacggacccg
     2221 gatctgcggg cacaactgca tcatcaggag gatacaatga aaggacgaca gagccgctat
     2281 gttaccggcg gagagagttt cgcggagatt gcccgtctcc cttcaggggc ggtggtgagg
     2341 ctctgtctga acaccggtct tgaggatgcg ctgcgggagg cctccaaatc gctcaagtca
     2401 gccttcaccc gttccgggcg aaaatgccgg ctgtcagcgg gtacggcgca ggggccgttt
     2461 accggacgcc ggcagggcgt ggccacacat ctcttcgtct cggtactctg agggggcagg
     2521 gggcaaaaaa agtaaaaatg tattcgccag gttgcccgga ggtgaaggaa aatagacata
     2581 cagcagaacg acggatagca ctttttgcta aatggacatc agtattacta tgctatagtt
     2641 gctttaatgg ataagtgcgc cttgacaaag gcggtgattt ctgttaacat tactctcata
     2701 gtattgttcc gtcccgctcc accccaacaa gatccgttta tttcccgcca gactggttat
     2761 caccattccg gcccggattt ttttggattt ttttccgggg agcccccgga acgagcttaa
     2821 aatcggtatg acaaacagga ggatgcgaat gaacacatca taacagagct gaaagataaa
     2881 acattctgta cggcattaac agcgttcacg tgtgtgaggc gccgggtgcc ttttgactta
     2941 aaaacgaggt tattgagatg accaaaatca agacagttac ttttgtaaat acttacccgg
     3001 gagggttcta tgaaaaactt gttagacacc gagggaacgg ttctattccc attccagact
     3061 gaaatccatt ttatttggac gattttctcc accgttaaac gcctggttat cggaaccagg
     3121 gaccatattt gccagaagca atactggagc gcctgtctct gtattttgct tcttatggcc
     3181 tatgtgggtc tctgtgctgc ggtggtctgg tttgtagtgc cctggctgaa ggtctttata
     3241 gtgtcgaaat ttgcggtttc ggcactatgg gtcacgccag taaagcgcgg actactctgg
     3301 ggtatcggta aagtggttac cgccacttgc cgaagattta ctctgctaaa gtaagtagcc
     3361 gcaacgctac acgaactgat ggtgaatgtc aacagatact caccatctcc ttacggcggt
     3421 ggtccctgtg accactgccg ctttcgcgtg ggtgcaacac ggcaaaactc ctctgtacaa
     3481 caggggctcc cgccgtcatt ttccggcaca ggtgaggccg gaattcggac taaaacgtaa
     3541 accgcgggcc agtccggtag cgttcactat cggccagcat tctctcaacc agagagaaat
     3601 ccttttcacc gcagaacacg tactgctccg cgaactccac ctgcagggaa ttgtaatgcg
     3661 tgacatgctc cggggtaagc ggcataagaa gatagtccgt atacctccgg atatgttcca
     3721 gtctttcaaa aggccgaatg tgacggggga taagatgcgg cgccctggcg agcagatgtt
     3781 gaagatgctg cttttgacga accatctgtt cacggatgga ggggcaatac atggccagca
     3841 tcagcgtcga tgacaggggg aggtaaatct gtatcccgcg aacggccaga ccaagattac
     3901 cgtacggacc gaagtcattg ctgttcttaa gcactacggg attgtctgat acgtaaaacg
     3961 gacgttctgg cctggtttcc agaaggtacc agtccttttt cagcaggaga tcaatatgtg
     4021 acacctgctg gacgagctgc cgcagaaaaa tatccttggt atcgcccccg gacgacaggc
     4081 ccagggtttc catggcctgc tctttagtcg agccgatggc ctccatctta tcggtaagca
     4141 ccgaaatcat gcctgagata cgctccagtt caccgaaggt tcgtgcgcgc tgcacggcaa
     4201 caaacacggc cagcctgtag cgttcatcat ctgtcagcct gcgaatatcc ctgtgcgcca
     4261 gcagggctgc aattaccggt gccgcgtggt gctcgtaaat ccccagcagt ggttcaagac
     4321 tcagacggtc cgggtgatta tcgaggttgt agaacgtgtt gcggacggtc gcgtcatccg
     4381 gcgtggtggc ataggcgcgc ccggcagcct tgtcaaaggc gtataagcgc tgccgcttac
     4441 cctttcccgg cgtactgaaa tgcttcagta aaaaacgggg aaccgtatgc tgcctgacca
     4501 catcggtctg cagattgaat ggatcggcca taactttatc tccctgaaaa gccacgcaag
     4561 cgcatcggtg catctgtatt gacggcatgg gcagctggtt tgaagcttag cattagaaaa
     4621 cagaaccgtt cgataatacc tgtgtttaac gccctgtcag taccgatgga atttaaatct
     4681 ccggaaccgc cggaaaccgg atctgcgcgg cagaaaagac gcaaatgata tgataataaa
     4741 aaatacacat aaaatcatat cagatatgat tttcatcatt gatgataaaa ccatatctga
     4801 tattatcaga aatcaggagc acacaaatca ttcaggatat gattattacc tgtcagaata
     4861 ttatcagatg gagcgtgcag gatggatcag gaaatgacgt tttcactcag ctatgaacaa
     4921 ctgacccggt ttgcggaacg acgcatccgg gaatgcaatc tgg
//
LOCUS       DAHBHC010000180         4962 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHBHC010000180 DAHBHC010000000
VERSION     DAHBHC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.184"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            77..379
                     /locus_tag="M2A35_005022"
     CDS             77..379
                     /locus_tag="M2A35_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201197.1"
                     /translation="MITVSVLPSKSQSISVSLAGQQCNIKIVQRQSFMYMDLTVNEVP
                     IMQGVPCLYGNKMVRYSYLGFKGDLIFIDNDGESDPQWEGLGSRYLLYYIEENELV"
     gene            300..1253
                     /locus_tag="M2A35_005023"
     CDS             300..1253
                     /locus_tag="M2A35_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201198.1"
                     /translation="MGNRTRNGKAWVAVTCCITSRKTSLYKNHSLRFDFTNESSAFDK
                     NGNNKISIRNVKSTVSLNFVTGRGGAQADISLYGLGIERLADISGKADGIVGEGQKLN
                     VEVFADDSLVFSGTMLSSIANMNSAPESSLMITASSNVDLQNMPASPFTAQGAQSVED
                     VISSICASAGYEAVFNNVKGMTTSGSPHFEGSVFEQLYRVCSDYGLAMSATPPTKVEF
                     WPSEKNRDDVIPFISREYGLVGYPVFSNGGLMFQTQYSSLLCIGRYVDIKTELPHASG
                     RYKLTSVRHELSSWMPNGSWHSICIAARTAEQRAEAQQNNG"
     gene            1246..1938
                     /locus_tag="M2A35_005024"
     CDS             1246..1938
                     /locus_tag="M2A35_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCB7201199.1"
                     /translation="MDKQLFTPTPAQVSEAQSQEYIFEMLMSGYFFIELARVQDVRGE
                     APDLVVDALPLLTRTDRTGKMISNSVLYDLPVWRLQRGNSAVIMDPVAGDIGLIAICD
                     KDTSLVRKNRKESVPGSKRRHSKSDAIYLGGVLNIAPTQFIEFADGAINITTPNPLNI
                     KCSSLNIDAPDGVNMNTPLLKVSGDIQDNASSQSSTVKNLRDNYNNHKHSVSGVQSGG
                     ATINSNATDKPT"
     gene            1935..2294
                     /locus_tag="M2A35_005025"
     CDS             1935..2294
                     /locus_tag="M2A35_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201200.1"
                     /translation="MTYRTMQLDTSTWDLTLDGNGNLAIAEESYSVAQDVASACLVFS
                     GECYYDNTLGIPWKAEVLGRRPSPGFIAQKMQAEALKLPVVEDALASVFFDKNTRTTR
                     GTIRVTDINGNIAQATL"
     gene            2291..3520
                     /locus_tag="M2A35_005026"
     CDS             2291..3520
                     /locus_tag="M2A35_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCB7201201.1"
                     /translation="MTILNTAVPDVTITENGLSVPDIADVLAGRLTDMSTALGGGASQ
                     SLSSPQGQIAQSDTEIIAQEYDKLLCLFNQINPDFSTGRFQDGIGRIYFMERIAAQGT
                     VVTATCIGQVGTTIPAGSTAVDTSGYIYQSIDNAVIPSSGSVDVQFVNTTTGPIPCAA
                     GALNQIYRAVSGWDAITNASPGVVGVDVESRIAFETRRKQSVARNSRNQDASTLSALL
                     ATNGVLDAYVWSNRTAATVNQGATNFPVVAHSIYICVYGGTDEDVAESIFQTYNPGAN
                     LNGDTSYTVYDNVNYLPPYPSYVMQWQKATPTRVYFSVELDRSLNPPSDITSQVKSMI
                     VSVFNGGYDGIGKERIGSTINAGKYYAPVISISPDTVGILSLEVSIDGSAYGPAITMG
                     IDQVPTIQESDITVTLS"
     gene            3531..4169
                     /locus_tag="M2A35_005027"
     CDS             3531..4169
                     /locus_tag="M2A35_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCB7201202.1"
                     /translation="MWEDTILTQYSASKKLLSIIDTFNQAVSLDDFTDEFIKKVWDLT
                     TCETFGLDMWGKIVGISRYINAPIETSSFGFSEADGKDLYYPNPFNDSPFFGGAQETT
                     NVRLSDDAYRTLIFCKAFTNISIATIPEINRFLNILFYQRGRAYCVNYRDMTIGITFE
                     FNLAPYEESILTNYDVTPVPSGVQVNIKQIVSPYFGFATDAYPFNDGTFYRN"
     gene            4463..>4962
                     /locus_tag="M2A35_005028"
     CDS             4463..>4962
                     /locus_tag="M2A35_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCB7201203.1"
                     /translation="MLISDDGERIFINTTEGNLSDPNSNGTGWKDILSYLGLGEGSAL
                     PVGVPVPWPSATPPTGWLKCNGADFSAEEYPELAKVYPTNKLPDLRGEFIRGWDDGRG
                     IDSGRPLLSVQNATIEEHSHGYNGAIYTSSGPSWANTTGAGYLEYSGFTSSYGGSETR
                     PRNIAF"
BASE COUNT         1466 a          987 c         1161 g         1348 t
ORIGIN      
        1 aaccaatact cgaagtcaca aacagcgcaa caaaaaaagc ggcagatatt gcaaaaaata
       61 tagttaaggc gagtacatga ttacagtatc tgttcttcca agcaaatctc agtccatttc
      121 ggtaagtctg gctggtcagc aatgcaatat aaaaattgtt cagcgacaga gttttatgta
      181 catggattta actgttaatg aagtcccaat tatgcagggc gttccatgtc tgtatgggaa
      241 taagatggta agatattcct accttggatt taagggagac cttattttca ttgataatga
      301 tggggaatcg gacccgcaat gggaaggctt gggtagccgt tacttgctgt attacatcga
      361 ggaaaacgag cttgtataaa aatcacagtc ttcggtttga cttcacgaat gaatcatcag
      421 catttgataa aaatggaaac aataaaatct ccatcagaaa tgttaagtca acagtatccc
      481 tgaactttgt taccggtcgg ggaggtgctc aggctgatat atctctttat gggttaggaa
      541 ttgaaagact tgctgatata tcagggaagg ctgatggtat tgttggggaa ggtcagaagc
      601 taaatgttga agtatttgcc gatgattctc ttgttttctc tggcacgatg ctttcatcaa
      661 tagcaaacat gaattctgct cctgaaagca gtctgatgat aacagcctct tctaatgttg
      721 atttacagaa catgccagca agcccgttta cagcgcaggg tgcgcaaagt gttgaagatg
      781 tcatctcatc catttgtgca agtgcaggtt atgaggctgt gtttaataac gtaaaaggga
      841 tgacaacatc aggaagtccg cattttgaag gaagtgtttt tgagcaactt tatagggtct
      901 gttcagatta tggactggcg atgtcagcca cgccaccgac aaaagtagag ttctggcctt
      961 cagaaaaaaa cagagacgat gtaattccgt ttatatccag agaatacgga cttgttggat
     1021 atcctgtatt ttcaaatggg ggactgatgt ttcagacgca atattcatct ctcctttgca
     1081 ttggaagata tgtggatatc aaaacagaac taccacatgc aagcggaagg tataagttaa
     1141 cctccgttcg gcatgagtta tcatcatgga tgccaaatgg ttcatggcac tcaatatgta
     1201 ttgccgccag aacagcggaa caaagagcag aggcgcaaca gaataatgga taagcaacta
     1261 tttactccaa cgcctgctca ggtaagtgag gctcaatctc aggaatatat ttttgagatg
     1321 ctgatgtctg gatatttctt cattgagtta gcgagagttc aggacgtcag gggggaggca
     1381 ccggatttag ttgttgacgc tcttccttta ctaaccagaa cagaccgtac aggaaaaatg
     1441 atttctaact cggttttata tgatcttccc gtatggcggt tgcagagagg aaacagcgct
     1501 gtaattatgg accctgtggc gggtgatatt ggtctgattg cgatatgcga caaagacaca
     1561 tcactggtaa gaaaaaatcg taaagaatca gtacctggaa gcaaaaggcg acacagtaaa
     1621 tctgatgcaa tttatcttgg tggggtatta aatattgcac ctactcaatt cattgaattt
     1681 gctgatggtg caataaacat aaccacgcct aacccactaa atattaaatg ttcatcgtta
     1741 aatattgatg ctcctgatgg agtcaacatg aatactcctt tacttaaagt tagcggtgat
     1801 attcaggata acgcaagttc acagtcatct actgttaaaa atttaaggga caattataac
     1861 aatcacaaac attctgtttc aggtgtacaa agtggtgggg caacaataaa ttccaatgca
     1921 acggataaac cgacatgaca tacagaacta tgcaactgga cacttctacg tgggacttga
     1981 cgcttgacgg aaatggcaat ctggcgatcg cagaagaatc atattctgtt gctcaggatg
     2041 tcgccagtgc gtgcctggtg ttttctggag agtgttatta cgacaatacg cttgggattc
     2101 catggaaggc agaagttctt ggcaggcgac catctcctgg ttttattgct cagaaaatgc
     2161 aggctgaagc actcaaatta cccgtcgttg aggatgctct ggcatcagtt ttctttgaca
     2221 agaatacacg tacaactcgc ggcacaatcc gcgttacaga tatcaatggt aatattgcac
     2281 aggccacctt atgacgatat taaatacggc tgttcctgat gtaaccatca ctgaaaacgg
     2341 tctttcagtt cccgacatag cggatgtatt ggcgggaaga cttacagaca tgtcaacggc
     2401 ccttggcggt ggcgcaagtc agtcactgag ttcaccgcaa gggcagatcg cccagtctga
     2461 cactgaaatt atcgctcagg aatacgacaa gcttctttgt ttgtttaatc aaattaaccc
     2521 tgacttttca accggcagat tccaggacgg gattggtcgg atttacttta tggagcggat
     2581 cgctgctcag gggacagttg ttacagcaac atgcattggt caggttggaa caacaatacc
     2641 agcagggagt accgctgtgg atacgagcgg gtatatatat cagtcaattg ataatgctgt
     2701 aataccctca tcaggctctg ttgatgtgca gtttgtcaac acaacaaccg gaccaatacc
     2761 ctgtgcagca ggtgcattaa atcagattta ccgtgcagta tctggatggg atgcgatcac
     2821 aaacgcgagc ccaggcgttg ttggcgttga tgttgaatca cgtattgcat ttgaaacacg
     2881 tcgcaagcag tcggtcgcga gaaatagccg caatcaggac gcatcgacac tatctgcctt
     2941 gctggctacg aatggtgtgc tggatgccta tgtatggtca aacagaactg cagccacagt
     3001 aaatcaggga gctactaact tccctgtggt ggcgcactct atttacatct gtgtgtatgg
     3061 tggaactgat gaggatgtag cggagtcaat tttccagacg tacaaccctg gagcaaatct
     3121 gaacggagat acctcgtaca cggtttacga caatgttaac tatctacctc catacccttc
     3181 ctatgtaatg cagtggcaaa aagcaactcc gacgagggtt tattttagtg ttgagctgga
     3241 tagatctctt aatccaccta gtgatatcac atcacaagtg aaaagcatga tcgtatcagt
     3301 tttcaatggt ggttatgatg ggatcgggaa ggaaaggata ggctctacca ttaacgccgg
     3361 taagtattac gctccggtta tttcaatatc acctgacacc gttgggattt tatcacttga
     3421 agtctccatt gatggatcag cctatgggcc agcaataaca atgggtatcg accaggttcc
     3481 gacaattcag gaatcagata ttaccgtaac tttatcgtga ggggtgaggc atgtgggaag
     3541 acacaatact tacccaatac tcagcaagta aaaaattatt gtccatcata gacacattta
     3601 accaggcagt gagccttgat gatttcactg atgagtttat taaaaaagta tgggatttga
     3661 ccacctgtga gacattcggg ttggacatgt gggggaaaat agttggaata agccgttata
     3721 ttaatgctcc aatagaaacc agttctttcg gtttcagtga agcagatggc aaagatctat
     3781 attacccaaa tcctttcaat gattcacctt tttttggtgg tgcacaggag acaacgaatg
     3841 tcaggctgag tgacgatgct tacagaactc tcatattttg caaagcattc acaaatataa
     3901 gtattgccac gatccctgaa ataaacaggt ttctaaatat attgttttat caaagaggga
     3961 gagcatattg cgtaaattat cgagatatga caataggaat aacgtttgag tttaatcttg
     4021 ctccctacga agagtcgatt ttaacaaatt acgatgtgac tcctgtacca agtggagttc
     4081 aggtaaatat aaaacaaatt gtaagcccat actttggttt tgctacagat gcatatccat
     4141 ttaatgacgg cacattttat aggaattaag catgaatcgt tctgaatcac ctaaaaaaca
     4201 acctaagcca tttggagtta atggacagag agtggcaata ttgccaacca cccctgcagg
     4261 agataactca gcatcgtatg aacaggggtt ccctccaata acaatgatcc tgaaatctgc
     4321 tggcggtttg ccgccaaaag gccaggatat gaaccaaatt ttatacgaac tttctgctct
     4381 ggggagatgg tcgagcaccg gggcgctgaa cacttttgat tctgaatttg cttcggagat
     4441 ttctggatat ccgtctgggg cgatgctcat atcggacgat ggggaaagaa tattcattaa
     4501 tacaacagaa ggaaatctat ctgatccaaa cagcaatggt accgggtgga aagatattct
     4561 ttcatacctt ggtttgggag aaggctctgc attacccgta ggtgtgcctg ttccatggcc
     4621 ttcagccact ccgccgacag gctggctgaa atgcaacggt gcagattttt ctgccgaaga
     4681 gtatccggaa ctggcaaaag tttatccgac aaataaattg ccagatttac gcggcgaatt
     4741 cattcgtggg tgggatgatg gaagaggaat tgattcagga cggcctctcc tgtctgttca
     4801 gaacgcgacc attgaggaac acagccatgg ttacaatggg gccatctata cttctagcgg
     4861 tccttcctgg gctaatacga cgggtgcggg atacctggaa tattcgggat ttacatcatc
     4921 atatggcggg agtgaaaccc gtccacgaaa tattgcattt aa
//
LOCUS       DAHBHC010000181         4911 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHBHC010000181 DAHBHC010000000
VERSION     DAHBHC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.185"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(166..1074)
                     /gene="galU"
                     /locus_tag="M2A35_005029"
     CDS             complement(166..1074)
                     /gene="galU"
                     /locus_tag="M2A35_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCB7201204.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1276..2289)
                     /gene="rssB"
                     /locus_tag="M2A35_005030"
     CDS             complement(1276..2289)
                     /gene="rssB"
                     /locus_tag="M2A35_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCB7201205.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2381..3286)
                     /gene="rssA"
                     /locus_tag="M2A35_005031"
     CDS             complement(2381..3286)
                     /gene="rssA"
                     /locus_tag="M2A35_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCB7201206.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCCIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3399..3857
                     /locus_tag="M2A35_005032"
     CDS             3399..3857
                     /locus_tag="M2A35_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCB7201207.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3907..4749
                     /gene="purU"
                     /locus_tag="M2A35_005033"
     CDS             3907..4749
                     /gene="purU"
                     /locus_tag="M2A35_005033"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCB7201208.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         1283 a         1235 c         1097 g         1296 t
ORIGIN      
        1 cgatttcctg atcaagcaat aatcttttgt agattgcact tgcttaaaat cccgccagcg
       61 gcgggatttt ttattgtccg gtttaagaca atttaataag aataagctat aaaaaaacgg
      121 cgtcgattgc tcaacgccgt ttcgtggata acaccgatac ggatgttact tcttaatgcc
      181 catctcttct tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta
      241 ttcaacgaag gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat
      301 atgataggct tccaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat
      361 ttcatcacca gctcccggag gggtttttgc cagcaacggc caaatatccg cgctaagtac
      421 gtaacgaccc acaatagcga gattagacgg cgcaacatcc gctttcggtt tttctaccac
      481 accaaccatc ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc
      541 atatgcggtc acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa
      601 gcggcggatc atctctgcca ggttatcctg tgacaaatcg gattcatatt catccagaat
      661 aacatcaggc aaaataacag ctaccggttc atcacccact accgggtgag cacacaatac
      721 cgcgtgtccc aggcctttcg ccagcccctg acgaacttgc ataatagtta cgtgcggcgg
      781 acaaatagac tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc
      841 cagttcaaaa ctggtatcaa agtggttttc aatagagttt ttagatgagt gagtaaccag
      901 cacaatttca gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt
      961 atcgacaagt ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc
     1021 taatcccgca acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat
     1081 tctcctggac tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc
     1141 agcaccccga aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac
     1201 ttatacgaat ggcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc
     1261 cgcgttatcg tttgctcatt ctgcagacaa catcaagcgc agtcgaccac cggttcccca
     1321 tatttggcat tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa
     1381 agtgcctaac ggaacaccat tactgatttg cacctggtgt tcgccggtat ttaacgtcgc
     1441 attcagaccc gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa
     1501 tagcggaaac tgccccggca gattggcctg acggagtaaa tggtttacct gcttcaataa
     1561 cgcgcccaac tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt
     1621 aaacaatgcg cgtaataaca aggcagcaag tacgccatta tgtccagctc gagtgacatc
     1681 aaggcaataa aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg
     1741 tttgtccgcg gcaaccaatt gacgataatt aacccggcaa tgggaaatca cctgctgaac
     1801 cggcggttgt agttcctgta ataatttcgc cgctgcggca gggttatcaa ccattgcatc
     1861 ccagtcgcga aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag
     1921 acaggcaaaa accatctcgc gcaagcgatt cagatcttta actggtttca gcaaaacatc
     1981 ttcaacgccc agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac
     2041 cagaactggg gtctggtcgc ctctgttacg tatatgctcc agcagtttaa gcccgttcat
     2101 tcgtggcatc gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag
     2161 ggcatccacc ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accatgaatc
     2221 cagaagcgag cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg
     2281 ctgcgtcatg ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccacctg
     2341 cgcctgtcag aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca
     2401 acggcaaaag ttcgtccatt ttcctttcca ctgccagctg tccggccgca atggcagcgt
     2461 gcgcgcgatg gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg
     2521 gcggatcacc tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg
     2581 tcataatctc tgttgccgtt ggcgctgtca ccgcccgacg tgtcgttatg ctgcctaacc
     2641 tttctttcag acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga
     2701 cattaaagga gagcaaatct tgttgcatca aatgagcatc gtgctgcagg tcaaccgcta
     2761 tcacaatatc agcccccaat gcacgcgtga gggaaatagg aattgggtta acgactgctc
     2821 catcaaccag ccagtagccg ttatgtgcaa caggtgccat gagtcctgga atacagcatg
     2881 atgcgcgaat agcaagatgg agatcgcctt cagtaaacca taattcacgt cccgtactta
     2941 aattggtggc aacagccgca aagcgacggg aacaattttc gatctctgtt tccggcatta
     3001 tttcgcggta ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga
     3061 gatccatcag gcgtaaaaca tcccaataac tgaaagaggt cacccaatct tccagcgcag
     3121 ataatcgatc gcatgcatag gcagcgccca ccagcgaacc aattgaacat cctgcaacga
     3181 tatcaatttc aatacccact ttttttagcg cattaataac gccaatatgc gaccaacctc
     3241 tcgccgcgcc agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat
     3301 tcccctggaa tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg
     3361 cggttaatac gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg
     3421 gtagtgctgt cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac
     3481 ctgatccaga acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt
     3541 atttaataaa gacctggcat ccctcttgtg gagcagcggc gttacgtgcc gaattgatgg
     3601 ccggatttgc gcatactgaa tggttgggat tgaccgtatt tgaacattgt tggcaggacg
     3661 ccgacaatat cggatttgtc agttttgttg ctcgatttac cgaaggtggt aaaacaggtg
     3721 caataattga acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta
     3781 cacgtccgca gtttggtcgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa
     3841 agtgctgcgg ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt
     3901 ccagcaatgc attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt
     3961 ctgatcgcac gtattaccaa tatttgctac aagcacgagt taaatatcgt acagaacaat
     4021 gaatttgttg atcaccgtac cgggcgcttt tttatgcgca cggaactgga agggattttt
     4081 aatgattcca ccctgctggc ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag
     4141 ctgaatcctg ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt
     4201 ggcgatttgt tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt
     4261 ggtaaccacg atactttacg ttctctggtt gagcgttttg atattccgtt tgagctggta
     4321 agccatgaag ggttaagccg caacgagcac gatcaaaaga tggcggacgc cattgatgct
     4381 tatcaacctg actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg
     4441 gcacgcttcc cgaataaaat catcaatatt caccattcat tcctgcctgc gtttatcggc
     4501 gcacgtcctt atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc aaccgctcac
     4561 tatgtgaatg acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat
     4621 catacctaca cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta
     4681 agtcgtgcgc tatacaaagt actggcacag cgcgtctttg tttacggtaa tcggacgatt
     4741 attctttaat cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac
     4801 gcaaccagtt catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc
     4861 gccccgcttc ccgataaggg agcaggccag taaaaagcat taccccgtgg t
//
LOCUS       DAHBHC010000182         4818 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHBHC010000182 DAHBHC010000000
VERSION     DAHBHC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.186"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(42..1346)
                     /gene="aceA"
                     /locus_tag="M2A35_005034"
     CDS             complement(42..1346)
                     /gene="aceA"
                     /locus_tag="M2A35_005034"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCB7201209.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(1376..2977)
                     /gene="aceB"
                     /locus_tag="M2A35_005035"
     CDS             complement(1376..2977)
                     /gene="aceB"
                     /locus_tag="M2A35_005035"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCB7201210.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(3246..4175)
                     /gene="metA"
                     /locus_tag="M2A35_005036"
     CDS             complement(3246..4175)
                     /gene="metA"
                     /locus_tag="M2A35_005036"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCB7201211.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            4332..4775
                     /locus_tag="M2A35_005037"
     CDS             4332..4775
                     /locus_tag="M2A35_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCB7201212.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1141 a         1222 c         1255 g         1200 t
ORIGIN      
        1 aacgccttat ccggcctaca gtcagcaacg gttgttgttg cttagaactg cgattcttca
       61 gtggagccgg tcagcgcggt gactgaagac gtgccgccct gaataatagt cgtcacttta
      121 tcgaagtaac ctgtacccac ttcctgctgg tgagatacga aggtatagcc atctttcgcg
      181 gcggcaaatt ccggctgctg cactttctca acgtagtgct tcataccctc gccctgggca
      241 taggcgtttg ccaggtcaaa catgttgaac cacatgctgt ggatacctgc cagggtgatg
      301 aactggaact tgtagcccat atccgacagc tgctgctgga agctggcaat agttttgtcg
      361 tcgaggtttt tctgccagtt gaacgacggc gagcagttat aagccagcag tttgcccgga
      421 tatttcgcgt ggatagcttg tgcaaagcga cgcgccagtt ccagatccgg cgtggaggtt
      481 tcacaccaga ccaggtcagc atatggcgca tacgccaggc cacggctgat cgcttgctca
      541 atgcccgcat gagtacggaa gaagccttca ctggtacgct cgccagtaat aaattcactg
      601 tcatacgggt cgcaatcgga ggtaatcaga tccgccgcat cggcatcggt acgagcaacc
      661 agcagggttg gaacgcccgt cacgtcagct gcaagacgcg ccgcgaccag tttctgaata
      721 gcttcctgag ttggcaccaa aactttgccg cccatgtgac cgcatttctt cactgacgcc
      781 agctgatctt cgaagtgaac tgccgctgca ccggcttcaa tcatcgcttt catcagttca
      841 aaggcattca ggacaccgcc aaaaccggct tccgcatcgg caacgatcgg caggaagtaa
      901 tcgacatagc gcggatcgcc cggctcaatg cctgcggacc attggatctg atcggcacga
      961 cggaaggtgt tgttgatccg ctccaccaca gctggcaccg agtttgccgg atagagcgac
     1021 tgatccggat acatgctggc cgccaggtta gcgtccgccg ctacctgcca tcccgacaga
     1081 tagactgctt caatacccgc tttcgcctgt tgcagcgcct gaccgccagt cagtgcaccg
     1141 aggctgttga tgtagccttt tttcgactcg ccatgcagca gacgccacat tttcgccgcg
     1201 cccagttgcg ccagggtgca ttcaggattg actgaaccgc gtaatttcac cacatcttcc
     1261 gcgctgtatg ggcgagtaat gccttcccaa cgcggttgag tccactcttt ctgtaattct
     1321 tcaatttgtt gtgtacgggt tttcatgtgc agatgctcca tagttatgtg gtggtttacg
     1381 ctaacaggcg gtagcctggc agggtcagga aatcaattaa ctcatcggaa gtggtgatct
     1441 gttccatcaa gcgtgcggca tcgtcaaaac gcccctggga gaaacgttct tcgcccagtt
     1501 cgctggcaat gactttcatc tcttcaccca gcatctggcg gaacaaggct ttggtcaccg
     1561 gtttgccatt gctcaacgtt ttttgatgat ggatccactg ccagattgag gtacgggaaa
     1621 tttcagccgt cgccgcatct tccatcaggc cataaatcgg cacgcagccg ttgccggaga
     1681 tccatgcttc gatgtactgc actgccacgc gaatgttggc gcgcatacct tcttcggtgc
     1741 gttcaccatc acaaggtgcc agcagctgat cggcagtaat cggcacgtct tgttcgcgca
     1801 tcacttcaag ctgattttta cgggagccga gaatgtcgtt gaataccgcc attgccgtat
     1861 ccgcaaggcc tgggtgagca atccatgtgc catcgtgacc gttattggct tccagcgctt
     1921 tatccgcttt tactttgttg agcacctggt tattgcgctc ttcatctttg ctcggaataa
     1981 acgctgccat gccgcccatc gcaaaagcac cgcgtttatg gcaggtttta atcaacaggc
     2041 gtgagtaagc attcaggaat ggtttatcca tcgtcactgc ctgtctgtct ggcaggacgc
     2101 gatcgggata gtttttcaac gttttgatat agctgaagat gtaatcccaa cgaccgcagt
     2161 tcagaccaac aatatggtca cgcagcgcgt gaaggatttc atccatctgg aacacggcgg
     2221 gcagcgtttc aatcagcaac gtcgccttga tggtgccgcg cggcagatta aagcgatctt
     2281 ctgcatagct gaagacttcg ctccaccagg ccgcttcctg ccaggactgg gttttcggca
     2341 gatagaaata gggaccgctg ccctttgcca acagtgcctg atagttgtgg aagaaataga
     2401 gcgcaaaatc aaacaggcta ccggggattg cctcaccacg ccaggtgaca tgtttttccg
     2461 gcaagtgcag accgcgtacc cgacaaatca aaaccgctgg attgggcttg agctgataaa
     2521 ttttgcctgc ttcattggta tagctgatgg tgccgttaac cgcatcgcgc aggttaattt
     2581 gcccgtcgat cactttgttc cagtctggtg ccagtgaatc ttcgaaatcg gccataaaga
     2641 ctttcacatt ggcgttcagt gcgttgatca ccatcttgcg ctcaaccggg ccagttatct
     2701 cgactcgacg atcttgtaag tccgcgggaa taccacgaat tttccagtcc gtatcgcgaa
     2761 tggaagctgt ttccgaaata aaatcaggca aagttccgtt atcaatatct tgctgctgct
     2821 gaatgcgcgc tgccagaagt ttattgcgtt gtggcgtaaa atgcgtcacc agctcagtca
     2881 gaaattctac cgcttcggca gtaagaattt gcttctcctg ctcgccatac ggccttatga
     2941 aagccagttc atcggttgtt gttgcctgtt cagtcatcgt gcagctcctc gtcatggatc
     3001 cgaaaacttc cccactgtga acgaaggatc gttgtgcgct tttcgttcag cacaactaag
     3061 actactcatt taaaatgcaa aataaaaaac aatttccatt ttaattaaaa atgcttgata
     3121 acttattgat aacaatatga ataaaattta ttcacctcct gaggtgcgtt tcggaaataa
     3181 aaaaggcacc cgaaggtgcc tgaggtaagg tgctgaatcg cttaacgatc gactatcaca
     3241 gaagattaat ccagcgttgg attcatgtgc cgtagatcgt atggcgtgat ctgatagacg
     3301 taatagttga gccagttggt aaacagcaaa ttaccgtgac tacgccagct cgctcgcggt
     3361 gtattttgcg gatcattgtg cgggaaatag ttatacggta cttccgggcc tagtccggct
     3421 tccacatcgc ggaaatattc ctgcgccagc gtttgcgcat catattcggg atggcccgtc
     3481 acaaaggcaa tgcgcttatc tttgctggca aacagatatg catccccttc ttccgtctct
     3541 gccagaattt ccagatcggt gtaatcacga atcaacgctg ccggaaagtc agcatagcgc
     3601 gaatgtggtg ccaggaatga atcatcaaag ccacgcgtca gaagcgcatg aggatggaga
     3661 atatgatgct cgtaaacgcc agagagtttg tcggtgcgag tttgcttagg aatgccgtag
     3721 aggatattga gcgcggcctg taccgcccag cagacaaaca gcgtcgaggt gacgtgatct
     3781 ttcgaccact ccagcacctg tttgatctgc ggccagtaag cgacatcatt aaactccacc
     3841 aggcccagcg gcgcaccggt tacaatcaga ccgtcaaagt tctgttcctg aatatcttca
     3901 aagttacagt agaagttgtt cagatgctct gcgggcgtgt tgcgcgattc acgggaatcg
     3961 atgcgcaaca gctgaatatc gacctgcaaa ggtgagtttg aaagcaggcg cagaaactga
     4021 ttttcagttt caatcttctt cggcatcagg ttaaggatca gaaccttaag tggacgaatt
     4081 tcctgaccag acgcacgaga agttgtcatc acaaagacgt tttcttcacg caagaaattg
     4141 acggcgggta gctcgtccgg cacacgaata ggcataacct gattacctca ctacatacgc
     4201 ttatacgttt agacatccag atagctgaag ataaccagaa aatttgccaa tgtcgagcct
     4261 gcatgttgaa ggtgagaaag tttcaaggaa aaggctacgt tagaatataa gaatgacgaa
     4321 aaggagagaa aatggttatt agtattcgcc gctcacagca tgaggaaggg gaggaactcg
     4381 ttgcgatttg gtgtcgatct gtcgatgcca cccacgattt tttatcagca gagtatcggg
     4441 ccgagctgga agagctggtt cgttcctttc tgccggaagc accgttgtgg gtcgcggtta
     4501 atgaacggga tcagccggtt gggtttatgt tgttaagtgg gcagcatatg gatgcgctgt
     4561 ttatcgatcc tgatgtgcgc ggctgcggcg tgggtcggat gctggtggag catgcgctct
     4621 cgatggcacc ggaactgaca accaacgtta atgagcaaaa tgagcaggcg gttgggttct
     4681 ataagaaggt gggttttaag gttacgggac gctctgaggt ggacgatttg gggaaaccgt
     4741 atccgttgct gaatctggcg tatgtggggg aataagccgc ccctgccgtg cctgcccagg
     4801 ctggagaaga cgcgccag
//
LOCUS       DAHBHC010000183         4795 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHBHC010000183 DAHBHC010000000
VERSION     DAHBHC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.187"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            356..736
                     /locus_tag="M2A35_005038"
     CDS             356..736
                     /locus_tag="M2A35_005038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201213.1"
                     /translation="MKKESGLSLIEAAIVLSLATAVVSGVLYFYNSAKENNAPPETTK
                     GLQSLIGATQALYGSRGQTAGTTLSAQAVSAISGIEIGSPKIAKGEQNVFYIPGRWQK
                     QPFSLTIIRQYLRYIQHQRIYVWL"
     gene            727..924
                     /locus_tag="M2A35_005039"
     CDS             727..924
                     /locus_tag="M2A35_005039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201214.1"
                     /translation="MALAGVNLGTMTTGKPRVSLSYGRQIYSSDYNSAISVSTPNDAA
                     SICGETTNTNLAHIMYELKVQ"
     gene            1022..1410
                     /locus_tag="M2A35_005040"
                     /pseudo
     CDS             1022..1410
                     /locus_tag="M2A35_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1421..2080)
                     /locus_tag="M2A35_005041"
                     /pseudo
     CDS             complement(1421..2080)
                     /locus_tag="M2A35_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2316..3092)
                     /locus_tag="M2A35_005042"
     CDS             complement(2316..3092)
                     /locus_tag="M2A35_005042"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201215.1"
                     /translation="MFDLITKTNDNIDEQSVSLSSYRIYKPVIVYTGAQRIFIQCKDT
                     GVSYYAEPDSLILLTKSNHSYNITAIKEFGGGRDVKIQYIDNDVIRSFSAVKFSSAVR
                     TKNVFIVKPDYHSIDLIYDVLSKENMSLFQFFLVLSYFGDDETIMKSLIGCSQCPLTD
                     KIIKMIEHDLSKNLSIIDVSKRLFVSESCLRKRLKKNNLTFKRISLDTKMKNASIQLR
                     TTSNSIYIIAESLGFGSVSYFIKVFKCYYGITPKQYKRCF"
     gene            complement(3507..3833)
                     /locus_tag="M2A35_005043"
                     /pseudo
     CDS             complement(3507..3833)
                     /locus_tag="M2A35_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(4124..>4795)
                     /locus_tag="M2A35_005044"
     CDS             complement(4124..>4795)
                     /locus_tag="M2A35_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCB7201216.1"
                     /translation="QRYGAPRLADELPEFNIKTIAASLRRQGLQAKASRKFSPVSYRA
                     HGLPVLENLLEQDFSASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPR
                     MTVQLACDALQMALWRRRRPESVIVHTDRGGQYCSGDYQAMLKRHNLRGSMSAKGYCY
                     DNACVESFFHSLKVECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFE
                     NHNLA"
BASE COUNT         1391 a         1045 c          994 g         1365 t
ORIGIN      
        1 cagatgtctg gaaatatagg ggcaaatcca gtccacatta cgtgggtagg atcagttttc
       61 tgcatcccgt attgggtaca atgatgccgg aaatctgcga ggctctatac ctctctgatt
      121 ttcccctttg tttatcactg tgctccagat ttaatgttgg gggacgttca tcttattccc
      181 ccggggcatg ctaacgccgg catttacggt ttctgtctgt tcttcagtaa aaataaagga
      241 aaccccgctc atatagatac tcccgaatta ttacaaaaaa gaattatttt ttctgtgtgc
      301 cacttgtttt ttgtgttatc ttttttggaa ttaaattgaa gtttcgagga tttatatgaa
      361 aaaagagtct ggcctgagtc taattgaagc tgcaatcgtg ctatctttag ctacagcggt
      421 agtttctggt gttttatatt tctataattc agcaaaagaa aataatgctc cgccagaaac
      481 tacaaaggga ctccaatccc tgattggggc tactcaagcg ttatacggta gcagaggaca
      541 gacagctgga acaactctta gtgcgcaagc cgtttccgct atttctggta tagaaatagg
      601 cagcccgaaa atagccaaag gtgaacaaaa tgttttctat attccgggga gatggcagaa
      661 gcaacctttt tccttaacaa taataagaca gtatttacgg tacatacagc atcaaaggat
      721 atatgtatgg ctttagccgg cgttaatttg ggaacaatga ctacaggaaa acccagggta
      781 agtcttagtt atggtagaca aatatatagt tctgattata actccgcaat atctgtaagt
      841 actccaaatg atgctgcatc gatatgtggt gaaactacaa acacaaattt agcacatata
      901 atgtatgagt taaaagttca gtaatgttga actttcaaaa gtgtaaataa atagaaacat
      961 agaggtttgt ccctgcactg acagacagcg acaaacctta actcacattt caagtttctc
     1021 aaggcaaatg ccagggatgt ggctggccga aaatgaaggg gcgaagttct ggctcaatgc
     1081 cctgactgaa ctgaaaaacc gcggtctgaa cgatatcctc atcgcatgtg tggatggcct
     1141 gaaaggcttc ccggatgcca tcaacacaat atatccggag gcccgcatcc ggttatgcat
     1201 cgtgcatgtg gtgcgtaaca gcctgcgctt tgtgtcatgg aaggacaaca aagccgtcac
     1261 ccgcgacctg aaagcaattt atcaggctcc ccggaagagg caggccggca ggcactgaaa
     1321 gcgttcgcgg cggcctggga cagtcgctat cctcagataa gccgaagctg gcaggctaac
     1381 tggccgaatc ttgccacgtt cttcgcttat tcgccggaca tttatctcga cccagcaggc
     1441 attcacctaa ataaattgct gtcgttgatg cttttgtgcc tgagtccgac accttttccc
     1501 agccatcacc aacattataa taatcattgc ccagtacccg gcaaatatag ctccagccac
     1561 gggagcggag ctgttggagc catcgcacct gaaaaccggc atctgtaata acaatgacat
     1621 tagtcccggt ggaaaagcac tcagcaagcg attccagaaa atgctcatgt acgtcagcat
     1681 tcgttgtctg cgatgatgga acgacacaac tcatcaacgg taaagaacgc ccgtcacatg
     1741 ctaagcttgc ccgcaaaagc tgaaaacgag aggcatgata agcactccag tcaatgagta
     1801 tcacaacacg agacattccc tgcataatct tctgggtaat tcgctgaaaa atggtggaaa
     1861 cctcattttg caaatgtcga ctccccaaca gtcgatccac tcgctttatt ttgtttttaa
     1921 ctgatgccgt atcggttaaa tgatgctcga tactggtaag cgtgagagaa gcggcattta
     1981 ttacagcatt agttgcgtca atcagtgatt tttgacggta aagacgtaaa ggagccagta
     2041 tgttgttaag gaaattttgg catgatgaac gtgcaggcat agtggtaatc tccttgaaat
     2101 tattagcata atcaattaga tcacatctca ctatacctgt cttgttttct agggattcct
     2161 ctgagcccta ccctcttgta gtaatatggt gtgttaaaaa taaaaatccc tttataaaac
     2221 tcgtaagagt tctagatctt ttgaaataat catacagttt aaaaaatgat tgtttttata
     2281 ttgagagatt atcctgctat aacatatata aatcatcaaa aacatctctt gtattgtttt
     2341 ggtgttatgc cataataaca tttaaatact ttgataaaat aagatacaga tccaaaccct
     2401 agtgattctg caatgatata aatgctatta ctggttgttc ttagttgtat tgaggcattt
     2461 ttcatttttg tgtcgagact aatccttttg aaagttaagt tgttcttttt taatcgcttg
     2521 cgtaagcatg attcacttac aaataatctt ttgctaacat ctattatact taggtttttt
     2581 gatagatcat gctctatcat tttaattatc ttatctgtta atggacattg tgaacatcca
     2641 attaggcttt tcataattgt ttcatcatca ccaaaatatg ataacacaag aaaaaactgg
     2701 aaaagtgaca tattttcttt tgataaaaca tcataaatta aatctataga atgataatca
     2761 ggttttacta taaatacatt tttagttctc acagcgcttg aaaattttac agcagaaaaa
     2821 gatctaataa catcattatc tatatactgt attttcacat ctctgccacc cccaaattct
     2881 tttatggctg ttatgttata agaatgatta cttttagtta gaagtatcaa cgaatctggc
     2941 tcagcataat atgaaacacc tgtatcttta cactgaataa atattctttg agctcctgtg
     3001 taaacaataa ccggcttata aattctatag ctactcaatg aaacgctctg ttcatcaatg
     3061 ttatcattcg tctttgtaat caaatcaaac atactaataa ttcataactc attttccaac
     3121 tgtgaacaaa aatatcaaaa aaaatcataa aaaaaacaca aaaagcccag caatgccatt
     3181 ttgttttatt tttttcacta taagtttcag taattcattt gtggtgcatt gacttcacac
     3241 tgtaatagtg acttatgtaa caatcacaca taatagtacc attcacattt aagggcattc
     3301 gtcataagtc ccgttattca aatgtgcaaa acaaattacg gagaaataag acaggctccg
     3361 gataacctta ttccagggat tttaagacgg gtaacctaac ctgagatgtc agatcgatat
     3421 cgcggagttc ctacttcctg cagggaaagt ctcgttatcg catgtggtgg actgcttcga
     3481 tggaatagtt gtgagcgggt tctccggtat cccatcgctt gatcacccgc ctgtggcttg
     3541 tattccatcc acaacagttg gcgcgtcacg ccaggttttc tcatttccgt atcgaagtac
     3601 agcatgtcag gcataacaaa ctccgacgca gaggtgtctt tgggcgggta aatgagtttc
     3661 tccagcgtgt catgggatat atcagcaggt aatggccatg acagagaaga ggctttgaag
     3721 cgtgcaagaa cctcaaaaac ggttgaggcg ctgatgtgta atgtcagccc gatacaacgg
     3781 ttagtcagct tctggtcgaa cttgagctga aggatcttaa aacaggtttc catagtgctc
     3841 ctggattttc tctttctgga cattgccgtc tccattggtt taaacacggc aatgaagact
     3901 gtggcgaaaa gagttaattc ccggagtgac tggcaggatg ccaatcaatg atcggaagca
     3961 tgccaaatcg tgatcgattg tgatgccaaa ctgtgatcgg agatggatgc caaatcatga
     4021 tcgattcaga tgccattttg cacggacagt gtcacgaagt acagcatgtc aggcataaca
     4081 agctctgacg cagaggtgat cctacccacg taatgtggac accctaagcg agattatggt
     4141 tttcaaattg ttccgggctg agaccgccac aggcactgtg acgacgccag cgattgtaat
     4201 cacactcgat ataattaaac accgttgccc gcattatttc ccggctgcta aagcgttccc
     4261 cgtggataca ttccaccttc agcgaatgaa agaagctttc cacacaggca ttgtcataac
     4321 agtagccttt cgcactcata ctgccacgca gattgtgtcg cttcagcatc gcctgataat
     4381 cccctgaaca gtattgacca ccacggtccg tatgaacaat gacgctttcc gggcgtcttc
     4441 tccgccacaa cgccatttgc agtgcatcac aggccagttg tactgtcatt cgcggtgaca
     4501 tcgaccagcc aatgacggcg cgtgaccaca ggtcgatgac tactgcgaga tacaaccagc
     4561 cctcatcggt acgcaagtat gtgatgtcac ccgcccactt ctggttcggg ccactggcgc
     4621 tgaagtcctg ctccagcaga ttctccaata cgggcaggcc atgtgcacgg taactgaccg
     4681 ggctgaactt ccggctggct ttcgcctgca gcccctgacg acgtaggctg gcggcaatgg
     4741 ttttaatatt gaactccggc agttcgtcag caaggcgggg agcaccgtat cgctg
//
LOCUS       DAHBHC010000184         4792 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHBHC010000184 DAHBHC010000000
VERSION     DAHBHC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.188"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..382)
                     /locus_tag="M2A35_005045"
     CDS             complement(<1..382)
                     /locus_tag="M2A35_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCB7201217.1"
                     /translation="MVFLVKKARRAVTEIKKFEKRDLAQVVINAAYLVAYADGECEAS
                     EKAKIEQVLRNQPALYAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTREA
                     EDVLDVAVAIAEADGEIEPEERKVL"
     gene            complement(560..633)
                     /locus_tag="M2A35_005046"
     tRNA            complement(560..633)
                     /locus_tag="M2A35_005046"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Arg,seq:tct)
     gene            complement(724..799)
                     /locus_tag="M2A35_005047"
     tRNA            complement(724..799)
                     /locus_tag="M2A35_005047"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(764..766),aa:Ile,seq:cat)
     gene            1065..1943
                     /locus_tag="M2A35_005048"
     CDS             1065..1943
                     /locus_tag="M2A35_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201218.1"
                     /translation="MTQQLENMPVTEIEIAYGGEAYADNQIDAKTLGEALTSLSALIE
                     HAEKIINGETAEPKVNIKATKEGSFTLLVAVMGSIKTINALGLVAGGGVAAGGVLGII
                     EWLKGRKISSIVVDEQKDTAEIEVDGEKVKCSNDIQKLITSPIIRKELDKLIYKPLQT
                     EKPSTFSVSQQKHKVVRVTQAEAVSFKTAKSTFVEKTHVTTRQANVHFANVRFKQGKS
                     WDIILPNGEEVSASMKDEAFLERVEHNQAAFCKGDLFVVELTETTKETNGALSKPRYS
                     ITKVIRHRAAADRKLL"
     gene            2193..2579
                     /locus_tag="M2A35_005049"
     CDS             2193..2579
                     /locus_tag="M2A35_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201219.1"
                     /translation="MANTKSYLAMQAWFTTLITAGLNFLFDWLPQLAFFKSLAPGAAV
                     GLSHVVILLIAYIGLPTLNDVRMKREIKTARKFITNCLANPNLTPDQIAHYNQCLIDL
                     DNKLLKNINIRIDALSDAESKAQANE"
     gene            complement(2593..2943)
                     /locus_tag="M2A35_005050"
     CDS             complement(2593..2943)
                     /locus_tag="M2A35_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7201220.1"
                     /translation="MGRDMYEVLDRWGAWAAAENSGVDWQPIAAGFKGLLPHGKKSRL
                     QCNDDEGIMIDGCVARLRKYKPEEYELLIAHFVIGVSLRSIAKKRRCSDGTIRKELQT
                     ALGFIDGCICLILS"
     gene            complement(2933..3304)
                     /locus_tag="M2A35_005051"
     CDS             complement(2933..3304)
                     /locus_tag="M2A35_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7201221.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKVGECYRRDVALIVRQQ
                     RLKLKLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKICEIRGDGNGA"
     gene            complement(3317..4366)
                     /locus_tag="M2A35_005052"
     CDS             complement(3317..4366)
                     /locus_tag="M2A35_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7201222.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIAPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGAGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVEDALPESVLRRSLGLRADKIRSVYRESDIVPGEQSATSILKQR
                     TKNLAPLLHTHQPQNPAQEKQVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGQPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4368..4646)
                     /locus_tag="M2A35_005053"
     CDS             complement(4368..4646)
                     /locus_tag="M2A35_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201223.1"
                     /translation="MAHDTKLHNSNDSAVFASRCGRRFHAFTSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSECPPRPSRTFQQRIWR"
BASE COUNT         1300 a         1226 c         1124 g         1142 t
ORIGIN      
        1 ccagcacctt gcgctcttcc ggctcaattt cgccgtctgc ctccgcaatg gccaccgcca
       61 catccagcac atcttccgct tcacgcgtat cgtgtttcac atcctcgatc tcacgtaacg
      121 ccgcacgacg accaatttta aagttcgtat ccagctgacc gataatggtt gcgctaatcg
      181 cattaatttc tgacgtaaac gcgtacagcg caggctgatt acgcagtacc tgttcgatct
      241 tcgctttctc ggaagcctca cattcaccat ctgcataggc caccaggtat gcggcgttaa
      301 tcaccacctg tgccagatcg cgtttttcaa actttttaat ttccgttacc gctctgcggg
      361 ctttttttac caaaaatacc aaacatcgtg acgttccttt gggtgggtga gccaacgccc
      421 gggagcgatc tgcccacaga gaaagtcaca ctgaccactc cgtaagctcc ccccccgaaa
      481 ggctctgtgg ttggtatgcg ccgggcgtgg cgcagataca aaaaaggccc gcaaaagcga
      541 gccgggaaaa taagtgtagc gcgttgtact ggagtcgaac caatgaccga ttgcttagaa
      601 ggcaattgct ctgtcctgct gagctaacaa cgcagaatac cgataatgga ccaccaccgg
      661 ggactcgaac ctcgcacact caacttaaag ggttgacgct ctttcctgat ggctagtggc
      721 ggttggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
      781 accactgagc taaagggccg ggcgcaggat aataacgtta cgaaatcaat gttgcaagca
      841 ttcaaaaatc acctggttaa aaatcaccct tatctcctcc accagcgcat tcaccatgtc
      901 tatccgagat aagtggcaca aaaaaacccg cttgatgcgg gtttaagttg tgtggcgaag
      961 cgaccactct taacagatta agatagtttt tgcgtacgcg ttagtgattt tttcataatt
     1021 atctgtatcc tgtggctatt tctcttgcgc aaaggatgct tttgatgacg cagcaactcg
     1081 aaaacatgcc agtaacagaa atcgaaattg cctacggtgg agaggcatac gcagataatc
     1141 agatcgacgc gaaaacatta ggcgaagcct taacctctct tagtgccctg attgaacacg
     1201 ccgaaaaaat aattaatggc gaaacagcag agccgaaagt caacatcaag gcaacaaaag
     1261 aaggttcatt tactttgctt gttgctgtga tggggagcat aaaaactatt aacgcccttg
     1321 gcctcgttgc aggtggtgga gttgccgcag gtggcgtact cgggataatc gaatggctca
     1381 aagggcgtaa aatcagctcc attgtcgtcg atgaacaaaa agatacagca gaaatcgaag
     1441 tggatggtga gaaggttaaa tgcagtaacg acattcaaaa attaataacc agtccaatca
     1501 tccgaaaaga actggataaa ttaatctata aacccctgca gactgaaaaa ccatccacgt
     1561 tctcagtttc acagcagaag cataaggtag tcagggtgac gcaagctgaa gcagtaagtt
     1621 ttaaaactgc aaaatccacc tttgtcgaga aaacgcacgt aacgacacgc caagctaatg
     1681 tacattttgc taatgttcga ttcaagcaag gcaaaagctg ggatatcatc ctgccaaatg
     1741 gcgaagaagt tagcgcatct atgaaagatg aggcttttct tgaacgggtt gaacacaacc
     1801 aagcagcatt ctgtaagggt gatctttttg ttgtagaact gacagaaaca acaaaagaaa
     1861 caaatggcgc gctgtctaaa ccacgctata gtattactaa ggttatacgc cacagagcgg
     1921 cggcagatag gaaactactt taaatcatgt caatcctaag cattatgctt tacatcactg
     1981 tattcatggc tgtcgcagcg acggtcagat atgctgtgct taggttcttg gtttgggtaa
     2041 agccgaatgc gtatattgaa ctaacctaca ccgatcctga aggcagaacc gctaaaagga
     2101 aagtcagcgt tagaaacgaa aatgacgcag aagagttggc gctactgcta cgcgaactca
     2161 aaactcgcaa cgaagcaagt gcaggaaggt gaatggcgaa cactaaatcc tacctggcaa
     2221 tgcaggcatg gtttaccacc cttatcactg cgggattgaa tttccttttt gactggcttc
     2281 ctcaattagc cttcttcaaa tcattggcgc ctggagctgc tgtcggctta tctcatgtgg
     2341 taattcttct tatcgcttac ataggtttgc ctacgcttaa tgatgtaaga atgaaaaggg
     2401 aaataaaaac ggccagaaaa ttcatcacga actgcctggc caaccctaac ctaacaccag
     2461 accaaattgc gcactacaac caatgtctta tcgatctaga caataagcta ttaaaaaaca
     2521 taaatattcg tattgatgct ttatctgacg ccgaatcgaa agctcaagca aacgagtagt
     2581 aacagtcata acttatgata gaatcaagca tatacatcca tcaataaagc ctaatgcagt
     2641 ttgcagctcc tttcttatag ttccatctga gcacctgcgt ttcttcgcga ttgagcgtag
     2701 agaaacacca ataacaaagt gagctatgag cagctcatat tcctctggtt tatactttcg
     2761 caaccgagcc acacaaccgt ctatcatgat gccttcatca tcattacact ggagacgtga
     2821 ctttttgcca tgtggcaaaa gccccttgaa gcccgctgct atcggttgcc aatcgacacc
     2881 gctattttct gctgcagccc atgcccccca gcgatctaaa acctcataca tatcacgccc
     2941 cattaccatc acctctgatt tcgcaaatct tcacgcccag tcgcccacca ggaacgagct
     3001 gaccgcgcac aatattgatt tcatcaaact gctcgtcgtc tatgagaagt ccggcatgcg
     3061 tcagcgcatc cagtggtgct ttcaggatat tgtccaggtc gcggcggcgc ttatccggtg
     3121 gctctgcaat aatctttatc gccagccttc cggacagttt taatttcagc cgctgctggc
     3181 gaacaataag cgccacatca cggcgataac actcaccgac ttttgataca aaatatgtgc
     3241 tgccacgacg tcgccagtaa gtattcaccg tcggcggata aggcaaaaca aactctatac
     3301 gcatcagtaa cctcttttac ccaagcacgc cggttgcaaa ggcgtgatca agaaaacgaa
     3361 aaattaaatc aacctgagaa ccatgctttt cttcgaacgc cagcggatcc gcatgaagtt
     3421 cgttgtgatg ttcccggcac aacggtagcg tgaaaatatc gtgggccttt gttcccatcc
     3481 ctccctgacc gtgaccaatc aggtgatggg gatcgtcggc tggttgacca caacacgcac
     3541 acggctgtgt cttcacccag cgcgtatatt tctcatttac ccaacggcga cgtttaggtc
     3601 gcctcatgaa agattccgga gactccggat caacggcaat gctgaccacc tgcttttcct
     3661 gtgctgggtt ctgtggctgg tgggtgtgaa gcaacggcgc aagatttttt gtgcgctgct
     3721 tcagtatgct ggtggcgctc tgctctcccg gtacaatgtc gctttcgcgg tacactgagc
     3781 ggattttgtc cgcacgtaac cccagagaac gacgtaatac tgactctggg agtgcatcct
     3841 ccacctgatt gcagaccgcc caccaggata attcagccag agataattcc cgctcctgcg
     3901 tgccattcat tgcatggcgt atgacgtcaa tcatccatgc tgacaaattt tggtgagcaa
     3961 gttgcccgag tgattcggag gtctggtcac gcaactggtt gtcgcagtgc cagcacaaca
     4021 ccatcgcgcc agcgccataa cggtgaatga cggtttcact gtggtgataa tcgccgtgtg
     4081 gccactggca ggatttaaca tggcgcaaca gccagtcaga cagtgcacca gcgccgccag
     4141 cagcacgaat cacacgctca ttgctgaaaa acggcagtaa tgatttatcc tccgccagcg
     4201 gctggcgaac ggcaggggcg atgccggacg gcagaccgcg catgcttttc ggttccggct
     4261 ccacaagcac tcgagggtta tggaatacct gcatggattc acggcctggt ttaaggacca
     4321 ccaacccaag ttccggtacc ggaacaggtc gaagtaatac ccgcacgtta cctccagatg
     4381 cgttgctgga atgtgcggga cggacgcggt gggcattcgg agtaagggag tctgactgag
     4441 attatccagt gacgatagtc gagactaaga gctttcttaa cctcgtatcc acgcctgcgg
     4501 taacactgaa ttatccattc agcctgctct tcagtgcatg gaggatgctg gaaccagtct
     4561 gatgtgaatg cgtgaaaacg ccgtccgcac ctgctggcaa agacagcaga atcattagaa
     4621 ttgtgtaatt tggtatcgtg cgccatcggt tgtctctgct ggcgcagcag gtgccagttg
     4681 ttcaggctgg cgtgcgaatt gtaaaccaaa atgccaggaa aaaacaaaac ccgccgaagc
     4741 gggttaagtg cgggtgcatt gaggatgcct gacacatcag aggtggcgag gg
//
LOCUS       DAHBHC010000185         4598 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHBHC010000185 DAHBHC010000000
VERSION     DAHBHC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.189"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1873
                     /locus_tag="M2A35_005054"
     CDS             <1..1873
                     /locus_tag="M2A35_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCB7201224.1"
                     /translation="KGNFDPLHLITEKYSTLRKYAPRMLSALQFVATPAAQPLSDALD
                     TIREIYRKQLRNVPPTAPTAFIPESWRKLVLTPSGINRKYYEFCVLNELKGALRSRDI
                     WVKGSRRYRNFDDYLIPVTEFDKSRQNDQLQLAVQTDCHAYLQARMTHLASRLEEVNA
                     MALAGELPDVDISDKGVKITPLENGVPSGVSAFASLVYNMLPHPKITEILEEVDSWTG
                     FTRHFTHLKNNNIRPKDGRLLLTTILADGINLGLTKMAESCPGVTKSSLEGIQAWYIR
                     DETYSAALAELVNAQGKRPLAAFWGDWTTSSSDGQNFRVGSHGRYAGQVNLKYGQEPG
                     VQIYTHISDQYSPFYTKVISRVRDSTHVLDGLLYHESDLEITEHYTDTAGFTEHIFAL
                     MHLLGFAFAPRIRDLHDKRLFIHGKAERYPGLQSVISTTSLNLKDIETYWGEVLRLAA
                     SIKQGTVTASLMMKKLASYPKQNGLAKALREIGRIERTLFMLDWFRDPGLRRRVQAGL
                     NKGEARNSLARAVFMHRLGEIRDRGLENQSYRASGLTLLTAAIALWNTVYIERAIESL
                     KRKKLPINDQLLSHLSPLGWEHINLNGDYVWRNNLKLGTGKYRPLRNVDIESYKKQS"
     gene            complement(2049..2123)
                     /gene="tap"
                     /locus_tag="M2A35_005055"
     CDS             complement(2049..2123)
                     /gene="tap"
                     /locus_tag="M2A35_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCB7201225.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCD"
     gene            complement(2356..2613)
                     /gene="repA"
                     /locus_tag="M2A35_005056"
     CDS             complement(2356..2613)
                     /gene="repA"
                     /locus_tag="M2A35_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCB7201226.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            complement(2923..3405)
                     /locus_tag="M2A35_005057"
     CDS             complement(2923..3405)
                     /locus_tag="M2A35_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCB7201227.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNAS
                     RTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(3396..3698)
                     /locus_tag="M2A35_005058"
     CDS             complement(3396..3698)
                     /locus_tag="M2A35_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCB7201228.1"
                     /translation="MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRT
                     DFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            complement(4019..4225)
                     /locus_tag="M2A35_005059"
     CDS             complement(4019..4225)
                     /locus_tag="M2A35_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCB7201229.1"
                     /translation="MKYLNTTDCSLFLAERSKFMTKYILIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
BASE COUNT         1284 a         1098 c         1045 g         1171 t
ORIGIN      
        1 taaaggtaat ttcgatcctc ttcacctgat caccgaaaaa tacagcacac ttcgaaaata
       61 cgctccacga atgttgtcag cattgcagtt tgtagcgaca cctgcggcgc agccgctcag
      121 cgatgcactg gatactataa gggaaatata ccgtaagcag cttcgtaacg ttccgcctac
      181 agcgccaaca gcatttattc ctgaaagctg gcgaaaactg gtattaacgc cttcaggcat
      241 caatcgaaaa tactatgagt tttgtgtact gaatgaactc aagggagcgt tacgttccag
      301 agatatttgg gtaaaagggt cgcgtcgtta cagaaatttt gatgattatc tcatcccggt
      361 cactgaattt gataaatccc gacaaaatga ccagttacaa ctggctgtgc agactgattg
      421 ccatgcatat cttcaggccc gtatgacgca tcttgcatct cgtctggaag aagttaacgc
      481 aatggctctt gccggtgagc tgccggatgt tgatatttca gataaaggag tgaaaatcac
      541 tccgctggag aacggggttc cttccggtgt ttctgccttt gccagtctgg tttataacat
      601 gctcccccat ccgaaaatta cagaaatact ggaagaagtt gacagttgga ctggttttac
      661 acgtcacttc acgcatctca aaaataataa catcaggcca aaagacggaa ggctgttgct
      721 gaccacaatt ctggctgacg gtattaatct ggggctgaca aaaatggcag agtcctgccc
      781 cggggtaaca aaatcgtccc tcgaaggtat ccaggcatgg tacatccgtg acgaaaccta
      841 ttcagccgcc cttgcagaac tggtaaacgc gcaggggaaa cgtccattag cggcattctg
      901 gggcgactgg acaacatcgt catcagacgg gcagaacttt cgggtgggca gtcacgggcg
      961 ttatgctggt caggtcaatc ttaaatatgg ccaggagccg ggcgtacaga tttatacgca
     1021 tatatcagat caatatagcc cgttctacac caaagtgatt agtcgggtac gtgactcaac
     1081 ccacgtactt gatggcctgt tgtatcatga aagtgatctg gaaattaccg aacattacac
     1141 cgataccgca ggtttcacag agcatatttt tgcactaatg catttgctgg gattcgcttt
     1201 tgcgccgagg atcagggatc ttcatgataa gcgactgttt attcacggaa aggctgagcg
     1261 atatccaggg cttcagtccg tcatatcaac aaccagcctg aatctcaaag atattgaaac
     1321 gtactggggt gaggtattac gcctggcagc ttcgattaaa caggggacag tcactgcatc
     1381 gctgatgatg aaaaagttag ccagttaccc aaaacagaat ggactggcca aagcgctgag
     1441 agaaattggt cgaatcgaac ggacactgtt tatgctggac tggtttcgag atcctggttt
     1501 gcgacgacgt gtacaagctg ggctgaataa aggagaagcc cgtaattctc ttgcgcgagc
     1561 ggtttttatg caccggctgg gtgaaatcag ggacaggggg ctggagaatc agagttaccg
     1621 ggccagtggt ctgacgttac tgacagcggc aatcgcgtta tggaacacgg tatacataga
     1681 aagagccatt gaatccctga aacgaaaaaa gctcccgatt aatgaccaac tgctctctca
     1741 cctttcgccg ttgggctggg agcatatcaa tcttaatggg gattatgtct ggcgtaataa
     1801 tcttaagctg ggtacaggga agtaccgacc attacgcaat gtcgatatcg aatcgtacaa
     1861 aaaacagtct tagcgtggga tattttccgt tttccaagcg gaccccatga atggcgaaat
     1921 caaaacggga ggtgaagccc accgccttct ccatcagttt ttcgcagaac ttcagcgttc
     1981 cggcaccttc acggggtgtg aacaccggat taggattctt tacctggcgg taatacgttt
     2041 gttgaagatc agtcacacca tccggcactt acaatgcaca gaaggagtga gcacagaaag
     2101 aagtcttgaa cttttccggg catataacta tactccccgc atagctgaat tgttggctat
     2161 acggttttat gaggccccgg taatctttct agtcgccaaa cttgaagaag attatcgggg
     2221 ttttcacttt tctggctcct gtaaatccac atcagaacca gttccctgcc accttacggc
     2281 gtggccagcc acgaaattcc ttaaacgatc agtaatctag cactgacaga aaaaactagc
     2341 aagaatgtga tcccatcaca tcatattccg gtgacaggcc tctttctcaa taagcctttc
     2401 tatcaactct gcctgagtta ccccttcctc tgcacacaga ctggtaaggt agtttttcag
     2461 ttctggtctg acgaaaacct tgatctcttt acatacagat cgtttgcgcg cagaagctgc
     2521 tctctgtcgc tctgcttcgc tcaaaggttt accctttctg tatgctcgtt ttgcgccaga
     2581 tgaggaagtt actgcatttt ctgtctgcga catctcgcct cctcaatact caaacaggga
     2641 ttatttcgca gagaatacta cagttttttg aaatcagcga cctgaacatt gtgacgatga
     2701 tcggtgttgg cgcacaaatc atgtctgatt acgaccgttt aatgtgattt agatagtgaa
     2761 atgactgaaa aacagaaaat agcgtaatca ggtacctgcc cggggtgggg ctggcggcat
     2821 tagagatatg gaagctaacc tgagggatta tcggttaaaa cttattagtc cccatgtgtt
     2881 acatgtcatt ataaaatgat gtcgcaataa aattgctcag cctcagactt tataaaacaa
     2941 ggtatttggt gccacaactg actggacaaa accaaaacgc tcataaaacg ctctggcatc
     3001 atcatcaatt gcatgaacca taacagcctt aataccaacc tgatcagcca gattgacaac
     3061 ccgatgaatg gcatcactca gcagccattt accaaaacca tgcccctgag tacaaacatc
     3121 aacagccaga cgccctaaca gaacaacagg aacaggattg ggcatgttat gccgcaaact
     3181 gcgcccaagt tcggcatgag tgacggaacc ggtagctaat gagtagtacc ccacaacacg
     3241 taaagtaccc tccaggcaaa tgacgaacgt gcgggaagca ttgagcatct ggtttttcat
     3301 agcgcgacca tgtaaccagt tacttaacac ttcattccca caatcaaatg gctgaagtat
     3361 atgctcatcc gtcagtaatg ccggtgcggt gacatttatt tccattcagg ctttaccgcc
     3421 atcagacgtt cacgcccttc agcattctgc acaggagctt caagttgcgt aataaatgaa
     3481 tcataacatt catcatccag cacaaataaa cgttgatcca ggatgatgtt tttagcttcc
     3541 tggtaagcca gttcaatgat gaaatcagta cggttcttat tgaccaggct agcggcataa
     3601 tcaataacag ctcgttcctc atccgttgcc cggatgttaa tctgattttc tcgcggcgtt
     3661 ttacggatgc ttgcagtagt aggcataaca acctccattg gtaacaattt gctatacaat
     3721 gaatgttatc gcaaatggta acagaacgct atacaaaatc actcatgggg gaaagtaatc
     3781 caccgataaa aataatgacg agcactaaaa gttgtaacga tgatctgtgt ttgggcacaa
     3841 atcatctccg attgcagaca tttaatatga ttttgattat taaatcactg aaaaacagga
     3901 aatgccttac ctgtgtgccg gccccgggcg ggaccggcgg tttctggcat gtcgggtgtc
     3961 tgcccctgaa atacctcaca cttccggaaa gcggggactt ccgtccccgc ccggcagctt
     4021 acttccgtgc ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca
     4081 gctcacataa ccgttctctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga
     4141 gcaacccaat taggatatat ttcgtcataa actttgacct ctctgcaagg aagaggctac
     4201 aatcagtagt gttaaggtac ttcatgcagg cctcacgagt taatggatta acaagtgggg
     4261 tcttcgcatt tctggtacct gcgtcttgcc tgaaccagaa aggctcagac acccgccgcg
     4321 ataataccac attccctgtt gctgactatt acccccattt acctgttttt gttacagaaa
     4381 cgccgggagc ggggaaaact tgctttttcc cgtttccggg gttggacaac tgagcaacgc
     4441 gaaggcgtca gctacgagtt gtcagcgcca gtgatataag acggtaattc accatttgga
     4501 ttgtccgctc cacccaacat gttgtttcct taaggttctc acaccagaaa ggacatcaac
     4561 atgctgagca gagaggactt ttacatgata aagcaaat
//
LOCUS       DAHBHC010000186         4396 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHBHC010000186 DAHBHC010000000
VERSION     DAHBHC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.190"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            16..159
                     /locus_tag="M2A35_005060"
     CDS             16..159
                     /locus_tag="M2A35_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCB7201230.1"
                     /translation="MSAIAAALHVHGVQRVEIIEPTQNMAISDIQAARCESFTISEGG
                     RNE"
     gene            152..760
                     /locus_tag="M2A35_005061"
     CDS             152..760
                     /locus_tag="M2A35_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCB7201231.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAVEPLGYLIEVKEW
                     WQLNEEPGTFRIVVGVLDQGITDEMYQELERLIADAKPASRHLTGLAISLSTTGNIFA
                     GAGCYHGDALTVYPYTPESIIVEGDYFPAPAIHLIDNLRVNA"
     gene            757..2241
                     /locus_tag="M2A35_005062"
     CDS             757..2241
                     /locus_tag="M2A35_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201232.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQNQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSAINSESETLAATPKAVKAAYDLASGKYTAQNATTTQKG
                     IVQLSSATNSTSETLAATPKAVKVVMDETNKKAPLNSPALTGTPTTPTAPQGTNNTQI
                     ASTAYVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTAL
                     AGLATAADKLPYFTGADRAALTALTSVGRAILSKPSTQGVLDYLGLGEAAKREVGTGA
                     NQIPDMASFTSGPGWMKFPDGTIIQFGVSIAGPIGYPTTVNFPIPFTSGYRIATSFDS
                     AINGATDCPAFATTPAGGGLLAFYLMSSRTGGTGAGANWIAIGK"
     gene            2244..2684
                     /locus_tag="M2A35_005063"
     CDS             2244..2684
                     /locus_tag="M2A35_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201233.1"
                     /translation="MNKYIYDAKTNAFYPLALKLHYESVGLWPEKGVEVDEETFSAFQ
                     KPPEGKMRIAGYDGYPVWSDIPPLAPEELIKMAEYERQRRIDTANEHMNNRQWPGKAA
                     IGRLKDDELEQYNLWLDYLDALELVDISSAPDIEWPTPPAIQAR"
     gene            complement(2656..3258)
                     /locus_tag="M2A35_005064"
     CDS             complement(2656..3258)
                     /locus_tag="M2A35_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201234.1"
                     /translation="MDNAVLNSELIATKAGNITVYNYDGKTREYISTSNEYLAIGVGI
                     PAYSCLDAPGTHKAGYAICRSMDLNSWEYVPDHRGEIVYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDEWNGEKWVTDTEAQHGAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3258..3776)
                     /locus_tag="M2A35_005065"
     CDS             complement(3258..3776)
                     /locus_tag="M2A35_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCB7201235.1"
                     /translation="MPVTRYLCIFTNVGLGEGSALPVGAPVPWPSETPPTGWLKCNGA
                     PFSAEEYPKLAKAYPANKLPDLRGEFIRGWDDGRGIDAGRVLLSIQTGMLEKHRHIVV
                     ANDGYDTKDEWELATIFKKTYTQGRGLDATNTGGSLIPSPTLHSRGSIGNTGGNETRP
                     RNIAFNFIVRAA"
     gene            3807..>4396
                     /locus_tag="M2A35_005066"
     CDS             3807..>4396
                     /locus_tag="M2A35_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7201236.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSARPG
                     LKKLLRTLSEGDTLVVWKLDRLGRSMKHLITLIEELREKGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARAQGRIGGRRPKLTKEQHEQIERLIKNGHD
                     RKQLAIIYDIGISTIYRYHPVGDIQAEETTGQTQGK"
BASE COUNT         1177 a         1067 c         1128 g         1024 t
ORIGIN      
        1 ggtcaggacg tggcgttgtc cgccattgct gccgcactgc atgtgcacgg tgtgcaacgc
       61 gtggagataa tcgaacccac acagaatatg gccatcagcg acatacaggc ggcgcgctgt
      121 gagtcattca ccatcagcga aggtgggcgc aatgagtaat tcgttgttac caccatcagc
      181 cagcaatttc atgcgttgtg ccgaagccgt cggaacacgc attacagaca ttccggtaga
      241 cctcaacacg ctgtggtcgc cggacacctg cccggtgcat ctgctgcctt atctcgcctg
      301 ggcgttttcc gttgaccgct gggatcgcaa ctggccggaa gagacaaagc gacaggtgat
      361 tcgtgatgca tggctgatac accgacacaa agggaccatc agcgcactgc gaagagccgt
      421 ggagcctctc ggctacctga ttgaagtaaa ggagtggtgg caactcaatg aggagcctgg
      481 aacatttcgc attgttgtcg gagtacttga tcagggcatc accgatgaaa tgtatcagga
      541 acttgagcgc cttattgcgg atgcaaaacc ggcaagccgc catctgacgg gactggctat
      601 cagtttaagt acaaccggca acatttttgc cggtgcggga tgctatcacg gcgacgccct
      661 gacggtttat ccctacaccc cggagtccat tattgtcgaa ggggattatt tccctgcccc
      721 ggccattcat ttaattgata atctgagagt aaacgcatga cagtgaaata ctacgccatt
      781 ctgactaatc agggcgcggc acgactggct aacgcgacga tgctcggcag taagctgaat
      841 ctgacgcaaa tggccgttgg tgatgcaaat ggtgtgttac caacaccaga ccctgcacaa
      901 acaaaactga ttaaccagaa acgcattgca ccgctgaatc ttctgagtgt tgacccgaac
      961 aaccagaacc agattattgc ggagcaaatc atccctgaga acgagggcgg attctggata
     1021 cgtgagattg ggctttatga tgatgaaggc gtactcattg cggtggcgaa ctgcccggaa
     1081 acgtacaaac cgcagttgca ggaaggcagt ggtcgtaccc agactatccg catgattctg
     1141 gttgtcacga ataccgaagc tatcacgctg aaaatcgacc cgtctgtggt tctggcaacc
     1201 cgcaaatatg tggatgacaa aatctcagag cacgaacagt cacgacgcca cccggacgcc
     1261 tcgctgaccg caaaaggttt cactcagtta agcagtgcaa ttaacagtga atcagaaaca
     1321 ctggccgcaa caccgaaagc ggttaaggct gcatatgacc tggctagcgg gaaatatacc
     1381 gcccagaatg ccaccacaac acaaaaaggg attgttcagc tcagtagcgc cacgaacagc
     1441 acgtctgaaa cactggcagc gacaccaaaa gccgttaagg tggtaatgga tgaaacaaac
     1501 aagaaagccc cattaaacag cccggcactg accggaacgc caacaacgcc aacagcgcca
     1561 caggggacta ataatactca gatcgcaagc acggcttatg ttatggccgc gattgccgcg
     1621 cttgtagact cgtcacctga cgcactgaac acgctgaacg agctggcggc ggcgctgggt
     1681 aacgacccga attttgcgac aaccatgact aacgcgcttg cgggtaagca accgaaggat
     1741 gctaccctga cggcgctggc aggacttgct acagcagcag ataaactccc ttattttaca
     1801 ggtgcagatc gcgccgcgtt aaccgcactg acaagtgttg gacgtgccat tcttagtaag
     1861 cccagcacgc agggggttct tgattacctt ggtttgggag aagcggcaaa acgggaggtt
     1921 ggaaccgggg caaatcagat cccggatatg gctagcttta cgtcggggcc aggctggatg
     1981 aaatttccgg atggtacaat catacagttt ggcgtcagta ttgcagggcc aatcggctat
     2041 ccaacaactg ttaacttccc gattcctttt acatctggtt atcgtattgc cacgtctttt
     2101 gacagtgcaa ttaacggtgc gacagattgc cctgcctttg cgacaactcc ggcaggaggc
     2161 ggactacttg cattttattt aatgagttct cgtactggag gaacgggtgc tggcgctaac
     2221 tggattgcaa tagggaaata aaaatgaaca aatatattta tgatgcaaaa actaacgcct
     2281 tctatccgtt ggctttgaaa ttacattatg aatccgtggg gttatggcca gaaaaaggcg
     2341 tggaggttga tgaagaaact ttttctgcat ttcagaagcc accggaggga aaaatgagga
     2401 ttgcgggtta tgatggttat cccgtatgga gtgatattcc gccactggca cctgaagaac
     2461 tgataaagat ggcagaatac gagcgtcagc ggcgcataga tactgccaac gagcacatga
     2521 ataacagaca atggcctggt aaagcggcta ttggtcgtct gaaagatgac gaactggagc
     2581 aatataattt gtggctggat tatctggacg cactggaact ggtcgatatt tccagtgctc
     2641 cagatattga atggcctacg cctccggcaa ttcaggccag atgacatccg gcgcggtgct
     2701 ggtatctgtt gccgtcaccg cgtcaatgta atccagcaca gcgttaagtc tggttgtttc
     2761 tgcctgtgtc agtttacgtc cggcctgcaa tttcagttga atcagactga tggaggccat
     2821 tgcagtatca atcagcgact ggcgctgtgc ttctgctgca tctactgctg cgccgtgctg
     2881 tgcctcggta tctgtcaccc atttctcacc attccattca tcgtatggcg ttaacggggc
     2941 gatagtggtt gtattttcag gataatcacc cggagctgtg atttctttcg attctcctgt
     3001 ttcggtgcta tagacaattt caccgcgatg atctggcaca tattcccatg agtttaaatc
     3061 catcgaacgg cagatagcat aacccgcctt atgtgtgcca ggggcatcta aacaggaata
     3121 tgcagggata ccgacaccaa tggcaagata ttcatttgaa gtggaaatat attcccgagt
     3181 tttaccatca tagttataga cggtaatatt ccccgccttc gtggcaataa gctcgctatt
     3241 taatacggcg ttatccatta tgcagccctc acgataaaat taaatgcaat atttcgtgga
     3301 cgggtttcat tcccgccagt attaccgata ctccctcgtg aatgaagtgt cggtgatggg
     3361 atcagactcc ctcctgtatt tgtggcatca agtccccgtc cttgtgtgta tgtctttttg
     3421 aaaatcgtag ccagttccca ttcatctttt gtgtcgtaac catcatttgc aacaacaata
     3481 tggcggtgtt tttccagcat ccctgtctga atgctcaata aaacacgtcc tgcatcaata
     3541 ccgcgcccgt catcccagcc acgaataaac tcaccgcgta aatcaggtaa tttatttgcc
     3601 gggtaagcct ttgccagttt cgggtattct tcagcagaaa aaggcgcacc gttgcatttc
     3661 agccagcctg ttggaggtgt ctcagaaggc cacggaacag gcgcaccaac aggtaatgca
     3721 gaaccttctc ccaaaccaac gtttgtgaaa atgcagagat aacgggtaac tggcatcatc
     3781 tccggttttt attcaggggg atgctcatgc ttattggtta tgttcgcgta tcaacaaatg
     3841 accagaacac ggaattgcag cgtaacgcgc tggagtgcgc aggatgtgaa ctgatttttg
     3901 aagataaaat cagcggaacg aaatcagcca gaccgggatt gaaaaaacta ctcagaacgc
     3961 tatcagaagg agatacgctg gttgtctgga agctggacag gctgggcagg agtatgaaac
     4021 acctgatcac gcttattgag gaattgcggg aaaaaggtgt taatttccgt agcctgacgg
     4081 acagcattga cacatcaaca cccatggggc gtttcttttt tcacgtcatg ggagctttag
     4141 cagaaatgga acgtgaatta attgtagagc gtacactggc cgggctggca gcagcacgag
     4201 cacagggacg cattggcgga cgtcgcccga agttgacaaa agaacaacat gagcaaatag
     4261 aaaggctgat taaaaacggc catgacagga aacaactggc gatcatttac gacatcggta
     4321 tatcgacgat ttatcgttat caccctgtag gcgatataca ggctgaagaa acaaccgggc
     4381 agactcaggg gaaata
//
LOCUS       DAHBHC010000187         4343 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHBHC010000187 DAHBHC010000000
VERSION     DAHBHC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.191"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..143
                     /locus_tag="M2A35_005067"
     CDS             <1..143
                     /locus_tag="M2A35_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201237.1"
                     /translation="GLACDVLRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKI
                     RK"
     gene            684..986
                     /locus_tag="M2A35_005068"
     CDS             684..986
                     /locus_tag="M2A35_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCB7201238.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            1033..1455
                     /locus_tag="M2A35_005069"
     CDS             1033..1455
                     /locus_tag="M2A35_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCB7201239.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            1452..1643
                     /locus_tag="M2A35_005070"
     CDS             1452..1643
                     /locus_tag="M2A35_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201240.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(1976..2044)
                     /locus_tag="M2A35_005071"
                     /pseudo
     CDS             complement(1976..2044)
                     /locus_tag="M2A35_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(2091..2292)
                     /locus_tag="M2A35_005072"
                     /pseudo
     CDS             complement(2091..2292)
                     /locus_tag="M2A35_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2413..2940
                     /locus_tag="M2A35_005073"
     CDS             2413..2940
                     /locus_tag="M2A35_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7201241.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            2998..3231
                     /locus_tag="M2A35_005074"
     CDS             2998..3231
                     /locus_tag="M2A35_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCB7201242.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            3290..>4343
                     /locus_tag="M2A35_005075"
     CDS             3290..>4343
                     /locus_tag="M2A35_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCB7201243.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQC"
BASE COUNT          892 a         1207 c         1372 g          872 t
ORIGIN      
        1 gtgggctggc ctgcgatgtg ctgcgtatac ccgatgaacc gccccgccgc tggtttgacc
       61 gtggtgttct gcgggaggtg gtccgggaag cccggaccga aatccgcagc atggcggatg
      121 ccctggcaaa aatccggaaa tgatgacact ggcggggtaa atccccgcct ttttcctgcc
      181 ggtccccgga cagagcacaa aggaagtcgc cgccggctcc gctttacccg gccatgcggg
      241 gcatggcctt gtgggttttc agttatgtgg ccaccgtgtc gtgtgcgggc tgtgccgtgc
      301 ctccatctta gccgggctgg cagggatgca agggtacgct tcgccgctgc ggtcacccgg
      361 tccctccttc ccgtctgccg tgattttccg gtcgttcacc cgctcaggtt tcgcggtctc
      421 accctccaac tcccgccagc cgtgtgcgtc aggctgcggc ttcccttgcg ccctgtgcat
      481 ccccgcctta tcgcccggct tttatggagg cacggcaccg cccggtgtcg cggaatatgt
      541 gaacaacgga ggaatacatc atgcaatatg cgaaacctgt cactctgaat gttgaagagt
      601 gcgaccgtct gttttttctg ccttacctgt ttggccagga ttttctgtat gtcgaagcgt
      661 ctgtgtacgc gctggcgaaa aaaatgatgc cggaatatga aggcggattc tggcacttca
      721 tccgcctgcc ggacggtggc ggctacatga tgccggatgg cgaccgtttc cacatagtga
      781 acggtgcaaa ctggtttgac cgtaccgtga gtgctgatgc ctgcggcatc attctgactt
      841 cccttgtgat taaccgccag ttgtggctgt accacgacag cggtgatgca ggactgaccc
      901 agctttaccg gatgcgcgat gcgcagttgt ggcgtcacat cgaatttcac cctgaatgca
      961 acgcgattta cgcggctctg gactgattaa cggacggggc ggcaatgccg cccctgtaaa
     1021 ggagacgaga aaatgtactg tactgttaaa gaaattatcc gcgaagtgct ggatacagac
     1081 gtgccggaca gtgaatgcgt ttttgccgtg gtgctgaccc gtggggatgt gcgtcacata
     1141 gcccaggact ggaacctgtc agacgatgag ctggaaaccg tcatgcagcg gctggacgat
     1201 gcctttgagc atggtgcgga tgtcagcgtt gttcacgacg ttgttcgtga actgatggaa
     1261 gaaaagcgcg tcagccgtca ggtgacagtc ccggcggtga tgctggaaaa agtggtggcg
     1321 ctggcaggca gtgaaatgaa gcgcctgtat gccgtcggga gtgagaacgg gggcgacggt
     1381 gacgcattcg tcagggaaga acgcgaagcg atggacgttg tgttacaggc gctggacggg
     1441 gagcacatgt catgaatatc agcacagaaa cccgcgaaat tctgcgcaat tacaaagccg
     1501 tgattaatgc gcggcgtcgt gagatggggc agaaaccgct caccactgcg cagattgttg
     1561 atgaaatctg cgattttgtg gtgaatcagc aggcggtttt cctcggtggt cactatatcc
     1621 ttcagggcag cagaaacagg tgatacaggg caggcggtgg aggccgcctg tcagcccgcg
     1681 cgaaaacccc tggcaggctg acgcctgccg gaaatgcggg gtgcagcgcc tgcggcgctg
     1741 ccggcccggc tttccggtca gaagatagtg tatggctgta tgccaggaac aggtgcgata
     1801 ttccgtgaca ctgtcactgt ttctggctgt cttttattgc gcccacttcg gctcccggct
     1861 gcctggcggc atccggtcgt caggcccgac tacgacaggc agctgcttgc gcacccgcca
     1921 gtctccgccg aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaaa
     1981 ccgggcttgc agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtgac ctgcgctccc
     2041 tgcacacctg acggtgtgtg aggaaactgc gcctgatggc gcagcagtct tccctcctgc
     2101 cgccacctct gcggcctgtt tcccgctgtg gactttccgg ggggcagacg taaccccctc
     2161 ccgttccatc tgtccagggt gagatgcacc ggccacaaaa tttttagcca tgaacccggc
     2221 aaaaaatttt gcgtccgccc ccggacagct gtcccgtgag gcggtaaacg ggcccccatc
     2281 ggaaaggtcc actgcgggga gaaacccctg tgaagtggac ggcaggagga gctttcgctc
     2341 ctctgaaggg gcacatgaac gtcccgtctc cggtatccgg tttatctcac atctttcagt
     2401 caggaggttc ttatgagtgc acgtggtatc aacaaggtca tcctcgtcgg gcgtctgggc
     2461 aatgatccgg aggtccgtta catccccaac ggtggcgcag tggcaaacct gcaggtggcc
     2521 acgtcagaaa gctggcgcga caaacagacg ggggagatgc gggagcagac ggaatggcac
     2581 cgtgttgtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg caagggcgcg
     2641 caggtctaca tcgaggggca actccgtacc cgtagctggg acgacaacgg catcacccgc
     2701 tacatcactg aaattcttgt taagaccacg ggcaccatgc agatgctggg gagtgcacca
     2761 cagcagaacg ctcaggcgca accgaagcct cagcagaatg ggcagccaca gagtgctgac
     2821 gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc gcagcccgag
     2881 cctcagccgc aaacgccgga gggggaggat tacgggtttt cagacgacat cccgttctga
     2941 tcgggctgac tgtgacaacc gccccgccct gtgcggggca tcaccggaga tatgaggatg
     3001 agcgaatatt tcagaatact tcagggactg ccggacggct cctttacccg cgaacaggcg
     3061 gaagccgttg ccgcacagta ccggaacgtc tttatcgagg atgaccacgg agaacatttt
     3121 cgcctggttg tccgtaataa cggaacaatg gtctggcgta cctggaattt tgaggacggt
     3181 gccgggtact ggatgaacca tgccatccgt gatttcggga ttcttaagta agagaggtgc
     3241 cggacgcgcg aacgtccggc aggacataag caattataag gggatgatta tgcctgtaac
     3301 gaagtgtgaa ccagaaacca cccgcaaagc aagccgtaaa tctgcaaaaa cgcaggaaac
     3361 tgccctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg cctcactgat
     3421 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgtg gagtccgtca gcgaactggc
     3481 tgggtccatt aagggcgttg gcctgttgca gaatctggtc gtgcataccc tgcctggtga
     3541 ccgttacggt gtcgccgcag gtggtcgccg actggctgca ctcaacatgc tggcagagcg
     3601 caacatcctt ccggctgact ggcctgtccg tgtgaaggtc atcccgcagg aactggcgac
     3661 tgccgcatcg atgaccgaga acggtcatcg tcgggatatg caccctgccg aacagattgc
     3721 cggattccgc gcattggcgc aggaaggcaa aacacctgca cagactggcg acctgctggg
     3781 ctattcaccc cgccacgttc agcgaatgct gaaactggca gaccttgcgc ctgtcatcct
     3841 cgatgcgctg gcagaggacc gcatcaccac cgagcactgt caggcgctgg cgctggagag
     3901 cgacaccgcg cgtcaggtgc aggtgtttga agcggcctgc cagtcaggat ggggcggtaa
     3961 accggatgtg cgggttatcc gcaacctgat tacggaaagt gaagtggcag tgaaggacaa
     4021 cactaaattc cgctttgtgg gggctgatgc cttctcgcca gacgaattgc gcaccgattt
     4081 gttcagcgat gacggggacg gttatgtcga ccgcgtggcg ctcgatgccg ccctgctgga
     4141 aaaactccag gctgttgcag agttccttcg ggaagccgaa ggctgggaat ggtgcgccgg
     4201 gcgcatggag cctgtcggtg agtgccgtga ggatgccgga acataccgct gtctgccgga
     4261 gccggaagcg gtgctgacgg aggcggaaga cgaacgcctg aacgaactga tgacgcgtta
     4321 cgacgcgctg gaaaaccagt gtg
//
LOCUS       DAHBHC010000188         4285 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHBHC010000188 DAHBHC010000000
VERSION     DAHBHC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.192"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            2..394
                     /locus_tag="M2A35_005076"
     CDS             2..394
                     /locus_tag="M2A35_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCB7201244.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="M2A35_005077"
     CDS             532..1416
                     /locus_tag="M2A35_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCB7201245.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="M2A35_005078"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="M2A35_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCB7201246.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATTPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..3403
                     /gene="tetR(A)"
                     /locus_tag="M2A35_005079"
     CDS             2753..3403
                     /gene="tetR(A)"
                     /locus_tag="M2A35_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCB7201247.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3435..3677)
                     /locus_tag="M2A35_005080"
     CDS             complement(3435..3677)
                     /locus_tag="M2A35_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCB7201248.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3735..>4285)
                     /locus_tag="M2A35_005081"
     CDS             complement(3735..>4285)
                     /locus_tag="M2A35_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCB7201249.1"
                     /translation="KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVAL
                     RELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQ
                     RYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDY
                     VWRQSRRLEDGKFRPLRMPGKP"
BASE COUNT          787 a         1337 c         1347 g          814 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg tcgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg agcagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccgatcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     3421 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     3481 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     3541 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg cgtccgctcg
     3601 tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg ggcgaggtcc
     3661 agggcggccg tcttcacgtt ctgccttgcg cagatgagat aggggagccc gcagaattcg
     3721 gaaaaaatcg tacgctaagg ttttccgggc atccgtaagg gccgaaactt cccgtcttcc
     3781 agtctgcggc tctgccgcca gacgtaatcg ccggttaggt tgatgtgctc ccagcccagc
     3841 ggcgacagga attgcagcag ctcgccgtcc accggcttgc cggcctcgac caacccctgg
     3901 gtggcgcgtt ccaggtacac cgtgttccac agcacgatag ccgccgtcac caggttgagg
     3961 ccgctggccc ggtagcgctg ctgctcgaag ctccgatccc tgatttcccc aaggcggttg
     4021 aagaacaccg ccctggccag cgagttgcgc gcctcacctt tgttcaggcc ggcatgcacg
     4081 cggcggcgca gttcaacact ttgcagccag tccaggatga acagcgtgcg ctcgatccgg
     4141 cccagctcgc gcagggccac ggccagtccg ttctggcgcg ggtagctgcc gagcttgcgc
     4201 agcatcagcg aggcggtgac ggtgccctgc ttgatcgagc tggccaggcg caggatgtcg
     4261 tcccagtggg cacgcacgtg cttga
//
LOCUS       DAHBHC010000189         4247 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHBHC010000189 DAHBHC010000000
VERSION     DAHBHC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.193"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..303
                     /locus_tag="M2A35_005082"
                     /pseudo
     CDS             1..303
                     /locus_tag="M2A35_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            301..484
                     /locus_tag="M2A35_005083"
                     /pseudo
     CDS             301..484
                     /locus_tag="M2A35_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1043..1621)
                     /locus_tag="M2A35_005084"
     CDS             complement(1043..1621)
                     /locus_tag="M2A35_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201250.1"
                     /translation="MKNISGSVYIWYPRHENIGHCSLQIGEEYFPENYVSWWPDGTAK
                     IFGKNDVLNSRFIDNGEIKQLPYILIPKEYDIQLEKEQPHVIYYLHQDCFLLEKMQTA
                     WQQIKTKNLPHYRMIGKNCADIVGRVLTEGASEVCRRYVVKKARPGLFTTPRDIASML
                     NHLAKEGGVQKEVSPSCPKRCRNFIYVCMKMR"
     gene            complement(1931..2074)
                     /locus_tag="M2A35_005085"
                     /pseudo
     CDS             complement(1931..2074)
                     /locus_tag="M2A35_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2143..3137)
                     /locus_tag="M2A35_005086"
                     /pseudo
     CDS             complement(2143..3137)
                     /locus_tag="M2A35_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3112..3241)
                     /locus_tag="M2A35_005087"
                     /pseudo
     CDS             complement(3112..3241)
                     /locus_tag="M2A35_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3241..4123)
                     /gene="istA"
                     /locus_tag="M2A35_005088"
                     /pseudo
     CDS             complement(3241..4123)
                     /gene="istA"
                     /locus_tag="M2A35_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1067 a         1068 c          975 g         1137 t
ORIGIN      
        1 gcactgatat caaaggtcac cgatgccgtg atggagcagg ttgtagaatg gcaaaaccga
       61 acactggatg ccgtttatcc cattgtttat cttgactgta tcgtcctgaa agttcggcag
      121 gacagccgcg tcatcaacaa atcggtgttc ctggcattgg gcatcaatat cgaaggtcag
      181 aaagaacagc tgggtatgtg gctggccgaa aaggaagggg cgaagttctg gttcaatgtg
      241 ctgacagagc tgaaaaaccg cggtctgaac gatatcctca tcgcctgtgt ggatgacctg
      301 aaagattaca tgttcttcgg caacgatcac ggtggagagc gtggtacgca actgtacacc
      361 tgccgactga acggtatcgc tccagaagcg tatctgcgct atatcctgag cgtgctgccg
      421 gaatggcctt ccaaccgtgt tgatgaactc ctgccatgga actgtagtac ttaccaataa
      481 ataagtctcg atacggcgct ccgttaacgt ttacgaataa gcgaagtgat cttttctgcc
      541 tctgatgacc ggctcacgaa cacggggaaa aacacaactc tcataatagc caatagagaa
      601 ataacactac atattgtgtt gacggactat ggtgcagtga gggagaagag gaagatccgt
      661 aacattcagt tcacgtacct gagccttctt ttctgctgtt ggctcagata atttcttaaa
      721 caacatcttc atccgtttcc tgactgacta tagggaaaca ctgtcagttc tgacattctt
      781 tagatttgta taaacaaggc tcgtacgatt ggtatcaccc gagacatttc ggcatttctg
      841 acaaagtccc cggagacaga cactgcccat ggccgaatgc tcctgtgcag tgtcctgcat
      901 caccaccctt agcgtatatc tgcaacgggc cgcgatctca cgttatgggc ttgatggtca
      961 ctccagatcc ggagtggcag agcttcttcc agaatggttc tgtatttgtt ctgacctgta
     1021 tccggtttaa tcggatcctt tgctatcgca ttttcataca tacatatata aaattgcgac
     1081 atcgttttgg acaggaaggg gaaacctctt tttgcactcc accttctttt gctaaatggt
     1141 taagcataga ggcgatatct cttggggttg taaatagtcc gggtcttgct tttttcacta
     1201 cataacgccg gcagacctca gatgctcctt cggtaagtac tcgccctaca atatctgcgc
     1261 aatttttccc aatcattcta taatgaggta aattttttgt ttttatctgc tgccatgcag
     1321 tctgcatttt ctccagcaaa aaacagtctt gatgtaaata ataaatgaca tgaggttgtt
     1381 ctttttcaag ctgaatatca tattccttag gaatgagaat gtacggtagc tgctttatct
     1441 caccattatc aataaagcga ctatttaaaa catcattttt tccaaaaatc tttgccgtac
     1501 catctggcca ccaactaaca tagttttcag gaaaatactc ttctcctatt tgcaatgaac
     1561 agtgtcctat attttcatgt ctgggatacc agatataaac gcttccacta atatttttca
     1621 tagttttccg ttgctcgctg agtgcttttc gataaacatt aataaactac ttacatccag
     1681 ttataacaga gtataattca ttaatatcat atactgtttt ctaaagccat aatgtgtgct
     1741 ccgaattttc tgtagttcag taatttacac tccctcaact gaccgggcac aaaaccaatt
     1801 aagagttttg ataatatgct gatatacatg caatgcatgt tctggcggtt atctgactga
     1861 gttgcgtttt cagtccgaga atttggttct gacatctagt aaatcttgtg cctgctgttc
     1921 ggtgctatcc gggcttaaca gccctgcacc atttatacag actatgtgcc aacacgccaa
     1981 gttgcccgga tacgtcacaa caaaacagcc tcatttaatt acttggcgaa caacctcttc
     2041 cttaaattca ggaggtaaaa cgtggtgtgc ccacagacgc ctcccgtacc caggctacag
     2101 ggcagattta tttgctccta cctatgtaat atggacacaa gcctaagcga ggttctgatt
     2161 ttcaaattgt tccagactga gaccgccaca ccaactgtgc cgtcgccacc gattgtaatc
     2221 acattctata taattaaaca ccattgtccg cattatttcc cggctgataa agtgttctcc
     2281 atggatgcat tccactttca gcgaatgaaa gatgctttcc atgcaggcat tatcgtagca
     2341 gtagcccttt gcgctcatac tgccacgcag attatgccgc ttcagtagca cctgataatc
     2401 tgctgaacag tactgacctc cacggtccgt gtgaacgaca acgttccaag gcctcttacg
     2461 ccgccacagc gccatctgca gggcatcgca ggccagttgc gccgtcatgc gtggtgacat
     2521 tgaccagcca ataacggcac gtgaccatag gtcaatgacc actgccagat acagccagcc
     2581 ttcatctgtt cgtaagtacg tgatgtctcc cgcccacttc tggttcgggc ttctggcgta
     2641 aaaatcctgc tccagcagat tttctgacac aggtagacca tgtacgtggc aactgaccgg
     2701 gctgaacttc cgggaggcct ttgccctcag cccctgacgg cgcaggcttg ccgctacggt
     2761 ttttacgtta aagtggtaac caagagcaaa cagtttatcc gtcagacgtg gtgcaccgta
     2821 acgctgtttt gactgggtaa aagcctcatg gacaacgctg tcacagtgct gacggaactg
     2881 ctgacgcttg cttatccttg tacgtcgctg acaccatgta taccagccgc tgcgggccac
     2941 ccagagcacg gcacattgct ttgatgctga actccgcctg atgtttttca ataaagacat
     3001 acttcatttc aggcgcttcg cgaagtccag ttgtagaatt gtgattcata caggctgagt
     3061 tcacgggctg cggcagcaac accgatgcgt tcagcaagtt ttagggcttt gaagcagatg
     3121 ctccaggaag tccatataac accattcctg gtccactgcc tgttgtgaca gcgcaggtgc
     3181 tgcgcttata aggctactgc ccggcgagtg ccatcagtcg ttgatgttgc agttccatca
     3241 ttacgccaca cctctgcaga atgagtcgta gatggagagt ggatgatgca gggggggcaa
     3301 cgttcaccag attttcacca agatgcacgt catactcttt tttctccgga ggcagtgcct
     3361 gcatggactg ctgctcttcg agccagcgat cgcaagaacg ggcctggatt atttcatgct
     3421 ttcgttggtt agcgacatcg tgcagccagc gcagaccgtg gcggttggct gtttcaacat
     3481 cgacagtgat ccccatcggg cgcaggcgag tcattagtgg gatgtaaaaa ctgttacggg
     3541 tgtactgcac catccgttcc accttacctt tagcctgtgc cctgaaggga cgacacagtc
     3601 ggggagagaa gcccatctcc ttgcctaact gccacagcga aggatggaac cggtgctgac
     3661 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     3721 gcggcacacc accaaagaag cggaacgcat tgcgatggca ggtctccagc gtatcataac
     3781 gcatattgtc agtgaattcg atgtacagca ttcggctgta tccgagaaca gcaacgaaca
     3841 cgtgaagcgg tgaacgacca ttatgcatag aaccccagtc aacctgcatc tgtcgtccgg
     3901 attcagtttc gaaccgaact gcaggctcct gctcctgagg aaccgagaga gaacggatga
     3961 acgccctgag aatggtcatt ccgccacgat atccctggtc tctgatctcg cgagagattt
     4021 ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata taatcccagt
     4081 attcatccag gagtgaggca actgcaggtc acggcgtatt ttcccggcac acggtcagca
     4141 ccggaccagc aacgagctgc gtaaacaggg cgtttttatc tccggcagtg gtgtccattc
     4201 tgtctggtta ggccataact gatcctaccc acgtaatatg gacacag
//
LOCUS       DAHBHC010000190         4184 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHBHC010000190 DAHBHC010000000
VERSION     DAHBHC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.194"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="M2A35_005089"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="M2A35_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCB7201251.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="M2A35_005090"
     tRNA            complement(324..399)
                     /locus_tag="M2A35_005090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="M2A35_005091"
     tRNA            complement(406..480)
                     /locus_tag="M2A35_005091"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(484..559)
                     /locus_tag="M2A35_005092"
     tRNA            complement(484..559)
                     /locus_tag="M2A35_005092"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Thr,seq:tgt)
     gene            921..1871
                     /gene="coaA"
                     /locus_tag="M2A35_005093"
                     /gene_synonym="panK"
     CDS             921..1871
                     /gene="coaA"
                     /locus_tag="M2A35_005093"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCB7201252.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(1900..2865)
                     /gene="birA"
                     /locus_tag="M2A35_005094"
     CDS             complement(1900..2865)
                     /gene="birA"
                     /locus_tag="M2A35_005094"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCB7201253.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2862..3890)
                     /gene="murB"
                     /locus_tag="M2A35_005095"
     CDS             complement(2862..3890)
                     /gene="murB"
                     /locus_tag="M2A35_005095"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCB7201254.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1083 a         1047 c          926 g         1128 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atatggtgcc gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta
      541 cctactgagc taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc
      601 aactaaaaaa ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac
      661 agccgaacgg agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgcgctc
      721 ctgctaaacc ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata
      781 gcttcttggc agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca
      841 ttaacccatt caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta
      901 tgaccgccag aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac
      961 agtttgaccg caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag
     1021 atgagatcgc ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga
     1081 tctatttacc tttgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg
     1141 cagttctgga acagtttctt ggtactaacg ggcaacgcat tccttacatt atcagtattg
     1201 ctggcagtgt cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc
     1261 gttggccgga acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc
     1321 aggttctgaa agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc
     1381 atcgcctggt gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg
     1441 tttactcgca tcttatttat gatgtgatcc cggatggaga taaaacggtt gttcagcctg
     1501 atattttaat tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc
     1561 cacatcatgt atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag
     1621 acttacttca gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttaccg
     1681 acccggattc ctattttcat aactacgcga aattaactaa agaagaagcg attaagactg
     1741 ccatgacatt gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc
     1801 gtgagcgcgc cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa gaggtcagac
     1861 tacgcaaata atttgcaggg gagcgaatac tcccctttct tatttttctg cactacgcag
     1921 ggatatttca ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcccc
     1981 ctgtttgtct attccacgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac
     2041 tgggcgatta ataaaattat ccagcttttc ccagcgcgac agataaggtg ccaatccttc
     2101 ttgttcgaag agttccaacg cagcacgtaa ttcacgtatt agcatggccg ccaacgtatt
     2161 acgatcgaga ttgatccccg cttcctgcag cgtgatccac ccctgattaa cgacactctc
     2221 ttcaacacgg cgcattgcca tgttgatccc ggctccaatg actatttgcg ccgcatcgcc
     2281 agttttgcca gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc
     2341 attaggccat ttgacacgaa ctttatctgc tcccagcttg cgtaatactt ccgccatcac
     2401 gataccgata accagactta aaccaatcgc cgccgccggg ccttgttcca gacgccagaa
     2461 catcgacaaa tataagtttg cgccaaaagg cgaaaaccat ttccgccccc ggcgaccacg
     2521 gccagcctgc tggtattctg caacacaggc atcgcccgat ttaagctctc cgatacgatc
     2581 aagaaggtac tgattcgtgg agtcaataac tggcagcacg gctacactac cgccatccag
     2641 ctgacccaat atctgttcag cattaagtaa ctggatgggc tcaggcaggc tgtatccttt
     2701 acccggaacg gtaaagacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat
     2761 agccgcccgg ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcaccgtt
     2821 cgctaacagg gcaatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact
     2881 gcgctcactt cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat
     2941 ttttcaccca ctttttgtct tacatggtgt gccagttgca ccacatcttc gctttttgca
     3001 ttgtcttcat taatgagaac taacgcctgt tgacggtgca ccgcagcccc acccatttgc
     3061 atccctttta gctggcactg atcaataagc caacctgctg ccagttttac tgaaccaccc
     3121 gcctgggggt aatttggtgc tgttggaaat tgcgccagta atgctttagc cgtttcggca
     3181 gatacaacag ggtttttgaa gaaactaccg gcattgccat tcacttttgg atcagggagt
     3241 ttggtggtgc gcatatgaca caccgcatta aatacttgct gtggcgttac tgttgtagga
     3301 tccagacgag ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga
     3361 cctacggcta caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtcgcgatag
     3421 ccaaaacggc actctttggc agttaagcgc acttgcttgc ctgtcgccag ttcaacacaa
     3481 tcaacataag cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt
     3541 gatgagccga cacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc
     3601 aaagtgtatt ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca
     3661 tcaggttcat catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag
     3721 tcttccagaa aaagtacatt acttccttca cccagaataa gaacgggttg tccttctgcg
     3781 gttgcatact gccaggcatt gagtaattgt tgttcgtctt cggcacatac aatgtgctga
     3841 gcattatgat caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc
     3901 ctgatgcaaa aacgaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg
     3961 acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt
     4021 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca
     4081 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     4141 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttt
//
LOCUS       DAHBHC010000191         4158 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHBHC010000191 DAHBHC010000000
VERSION     DAHBHC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.195"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(111..1268)
                     /gene="intS"
                     /locus_tag="M2A35_005096"
     CDS             complement(111..1268)
                     /gene="intS"
                     /locus_tag="M2A35_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCB7201255.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSIGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITINANVMKGRKIHVVPMSDQVVE
                     LLTMLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(1430..1504)
                     /locus_tag="M2A35_005097"
     tRNA            complement(1430..1504)
                     /locus_tag="M2A35_005097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Arg,seq:cct)
     gene            complement(1580..2512)
                     /locus_tag="M2A35_005098"
     CDS             complement(1580..2512)
                     /locus_tag="M2A35_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCB7201256.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2806..3561
                     /gene="mlaA"
                     /locus_tag="M2A35_005099"
     CDS             2806..3561
                     /gene="mlaA"
                     /locus_tag="M2A35_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCB7201257.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3743..>4158)
                     /locus_tag="M2A35_005100"
     CDS             complement(3743..>4158)
                     /locus_tag="M2A35_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201258.1"
                     /translation="NGDSLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELN
                     GERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDF
                     KDLEDKVEKRLQNRQTEYQNEINQLSAPGVNFDDI"
BASE COUNT         1025 a          976 c          946 g         1211 t
ORIGIN      
        1 agccgttatc gattttacta tgttttcagt agaacactca gacaaaactg agacaaacaa
       61 agctttgcac tggattacaa ggctttgtgt cattcgatag ctaaggtgga tcactccacc
      121 ttttcatcaa tccagtccgc ccaccactgc atcatttctc tgcgcttatc tagatactga
      181 gcatggttgt aaatcccgcg cacagaaccg ccgttggcat gtgccagttg cacttcaata
      241 gcatcagcag gccattcgtg ctcgttcata atcgtgctga attcatgcct gaatccgtga
      301 ccgctttcca gaccttcata gccgatttgt ttgatcacaa gcagtacagc gttctcgcaa
      361 attggcttct tcttatcgtt gcgcccggca aaaacaaact ctgatactgg tttggtgatt
      421 gagcttagca tagtgagaag ttcaactacc tggtctgaca taggaaccac atgaattttg
      481 cgtcccttca tcacattggc gttgatggtg ataatcctgt tttcaaaatc gacgttcttc
      541 catagcatgg aacgaagctc ttttgttctg agggctgtgt agcgtaaaac tttggtcgca
      601 atgagcgata cgatgcttcc tgaaaatgtt gccagtgctt tgttaaatgc cgggatctgg
      661 tctgcaggaa gaaacgggaa gtttttcttg cggtatccct tcatggcgtc agcaaggtca
      721 ggtgccgggt tatatttagc cctaccagtg acaatagcgt aacggaaaac ctctccgcat
      781 cttctgcggg ctttatttgc tcgctccatt gcaccgcgat cttcaaatct gcggattact
      841 tccagcagtt gcatcggctc aatatcctga atttcaaggc cgccgatgat aggtaaaatg
      901 tcgtcatcaa acatttttgc aagttcagtt gcatagccta ttgaccagac ttgcttcttg
      961 tgctcgtacc attccttgta aatcgcacta aaggaattgt tgttagacga agcctttttc
     1021 gcttttaccg gatcgatgcc aaccgagatg tctttcctcg cggtccatgc tttatctctt
     1081 gcctcctgca aagtcataag cggatatttt cctacggtca ggattttttc cttaccgtca
     1141 atcttgtagc gaagctgcca tacctttttc ccggatacag ggacataaag gtacaggcca
     1201 ttaccatcga gaaggcgata tggtttttct ttcggctttg ctgcttcaat ctgcttaacg
     1261 gtgagcatgg gtaaaaatcc ggtgggtaaa attattttat ccacttttta cccgtcatgg
     1321 agtgcggctg tcaacgatct gacgcgaacc atgacgaact gcgaatctac ggaaggcttg
     1381 atattcaggg gattttgcgg actgttacgg atgggagcga actgataaat ggtgtcccct
     1441 gcaggaatcg aacctgcaat tagcccttag gaggggctcg ttatatccat ttaactaaga
     1501 ggacaatgcg gcatgagtat acccgctaat ggactgcggg gtaagtacgc tgccgctcga
     1561 ttgcttaaac tctcgccatt tatgctgggt ttttatcatt tttcttaatg ttttccgcac
     1621 gttctgcttt ttggcgtgct tctgctttac gcttgttgct catgtcgtta cgaatctgtg
     1681 catgactcat taacgcgaag ataaaggtgc cgccgcagat gttccccgct aaagtaggta
     1741 gtgcgaaggg ccagatgaaa tcgctccagt gcagcgtgcc gttaaacacc agatagagga
     1801 tttcaacaga accgacaacg atgtgggtgg tgtcacccag tgcaataagc caggtcatca
     1861 atataatcac cacaatcttt gccgcacccg cagcaggaaa catccaaacc atagtggcga
     1921 tcagccagcc ggaaatgatc gcgttggcaa acatctcgct gggggtgttc ttcatcacat
     1981 ccatgccgat tttgacaaat gcatcgcgag tttcttcatt gaagataggc atatattcaa
     2041 atgcccatgc agcaatacct gtcccgagaa tattacccag cagcacgacg ccccataatc
     2101 gcataagtaa gccgacgttg ctcattgtcg gtttttgcat gacgggtagt accgcagtca
     2161 cggtgttttc ggtaaataat tgctggcggg ccataatgac gataataaaa ccaaaggtat
     2221 aaccgagatt ctccagtaag aagctgcctg gcactccttc cagttcgaca tgaaatatcc
     2281 cttttgccag tagcgaagcg cccatcgaca gacccgccgc aatggctgac cacagtagcg
     2341 ccattgcgtc gcgttccagc tctttttcac catcctggcg gatatgctca tgaattgcca
     2401 tcgcccggga ggggagtcgg tcttcatcta tttctatttt tttgccgcgc tctttttctt
     2461 cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt
     2521 ctcttgaatt agcacgtata gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag
     2581 ttactctgaa aatgtagaaa aggctgcgtt tgcctttttc tgtttctata gaatcaagta
     2641 gcctacaggg cggcgattac caggctatga tcaaatcagc aaatcagggc gtctggacat
     2701 cagttgacgt gctgttacaa tcgcccacac ctaaacaggc ggatacggta tcgttccgtc
     2761 atggatggca aactgcataa gccataaaaa aacagggaga catttatgaa gcttcgcctg
     2821 tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg cgagttccgg tacagatcag
     2881 caagggcgtt ctgacccgtt agaagggttc aaccgcacca tgtacaactt caacttcaat
     2941 gtattagacc cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa
     3001 ccggcgcgta acggtttgag caactttact ggcaaccttg aagaacctgc ggtgatggtt
     3061 aactacttct tgcagggcga cccttatcag gggatggtcc actttacccg ctttttcctg
     3121 aacaccattt tggggatggg cggttttatt gatgttgcag ggatggcgaa cccgaaactg
     3181 caacggactg aacctcaccg cttcggtagt acgcttggtc attatggcgt gggttatggg
     3241 ccttacgtcc agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg
     3301 gcggatggtc tttacccggt tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg
     3361 acgcttgaag ggatagaaac tcgtgcgcag ttgttggatt ccgatggtct gctgcgtcag
     3421 tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct
     3481 aatggcggcg aactcaaacc gcaggaaaat ccgaacgcgc aagcgattca ggatgattta
     3541 aaagatatcg attctgaata agaaacaaat aaaaaaggtg agtcgcaata ctcacctttt
     3601 ttgttgttat ccctcaatta ccacaagtaa aaggagataa accgacaatg acccggacgt
     3661 aatgatgtaa aaatactgat gttcgtcact gactatttcc gatattcagg agttgaaaaa
     3721 tcaaaaaact ggaaaaataa ttttaaatat catcaaaatt aacacctggc gcagatagtt
     3781 gattaatttc attttgatat tccgtctggc gattttgaag ccttttttca actttatctt
     3841 cgagatcctt aaagtctttg tatttatttt caggatggtc aacgtcaact ttgttagcga
     3901 ataaagtaga aattatttct tttacattat cgcgtctaaa ttttgcatcg tccacttgaa
     3961 aattatatct tattgctcga cctaaacgtt taaaaacttc gttaggttct ctcagtcttt
     4021 ccccgttgag ctcgtttaca acctgatgat cggaacctgt tgcaaatgat tctgccatga
     4081 tagctttact gctttcaaac cacgctgaag cattcctggc tttttcacta caatattggg
     4141 tgagactatc accattaa
//
LOCUS       DAHBHC010000192         4084 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHBHC010000192 DAHBHC010000000
VERSION     DAHBHC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.196"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1964
                     /gene="dinG"
                     /locus_tag="M2A35_005101"
     CDS             <1..1964
                     /gene="dinG"
                     /locus_tag="M2A35_005101"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCB7201259.1"
                     /translation="YLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKKIIPDL
                     KFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLKGDLDT
                     YKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAEVVVAN
                     HALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQLDLFTK
                     LVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEHRFAMG
                     ELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRALGMFE
                     AQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWRSIPHI
                     IVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVEPSIDN
                     EEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGDQPRYR
                     LVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVITEGEW
                     LKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKRLLDAL
                     PVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            1992..2954
                     /gene="ybiB"
                     /locus_tag="M2A35_005102"
     CDS             1992..2954
                     /gene="ybiB"
                     /locus_tag="M2A35_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCB7201260.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(3133..3795)
                     /locus_tag="M2A35_005103"
     CDS             complement(3133..3795)
                     /locus_tag="M2A35_005103"
                     /inference="COORDINATES: protein motif:HMM:NF022337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCB7201261.1"
                     /translation="MFPKIPLWQIPLALLLSVIALFFFYLLFLALYSLIRGLTDKKLK
                     KESIHKTLHHAPDNTPNYAPPLFNYKATSKKSHTLQYDAFQFYQKKNFMSNRERRFFK
                     LIYDEFHQDFFVFSQVRMVDIIVPIFDKNTQYNSFIKAFRSISQYHIDFLIVNKNDFS
                     IICAIELDDYSHDLPDRVDRDQKVNMIFKFTGITLLRSRNLEELLNKIRHHIQHHNDE
                     IT"
BASE COUNT         1078 a          918 c         1068 g         1020 t
ORIGIN      
        1 cctatttgat ccccggcatc gccattgccc gcgaagagca aaaaacgctg gtggtgagta
       61 ccgccaacgt ggcgttgcag gatcagattt acagcaaaga tttaccgctg ctgaaaaaga
      121 tcattcccga tcttaaattc actgccgctt ttgggcgtgg gcgctacgtt tgcccgcgta
      181 atctgacggc gctcgccagc actgagccca cgcaacagga tctgctggcg tttcttgacg
      241 acgaactgac gccaaataat caggaagagc aaaaacgttg tgcgaagctg aagggcgatc
      301 tcgacactta taaatgggat ggtctgcgcg atcatactga tatcgctata gatgacgatc
      361 tctggcgtcg tttaagtacc gacaaagcca gctgcctcaa ccgcaactgt tactactatc
      421 gtgaatgccc gttttttgtt gctcgtcggg agattcagga agcggaagtg gtggtggcaa
      481 accatgcgct ggtgatggcg gcgatggaaa gcgaagccgt actacctgac ccgaaaaatc
      541 tgctgctggt gctggacgaa ggtcatcacc tgccagatgt ggcccgggat gcgctggaga
      601 tgagtgccga aatcaccgcg ccctggtatc gactacagct ggacttgttc acgaaactgg
      661 tcgctacctg catggagcag tttcgcccga agaccatccc accgctggcg atccctgaac
      721 gtttgaatgc gcattgtgaa gagttgtatg agcttatcgc ctcgttaaac aacattctca
      781 atctctacat gcctgccggg caggaggcag agcaccgttt tgcgatgggc gaactgcctg
      841 atgaagtgct ggagatctgc cagcggctgg caaaactcac cgagatgctg cgtggtctgg
      901 cggagttatt tcttaacgat ttaagtgaga aaaccggcag ccacgatatt gtgcgtctgc
      961 atcggttgat tttgcagatg aaccgcgcgt tggggatgtt cgaggcgcaa agcaaactct
     1021 ggcggctggc gtctctggcg caatcttccg gtgcgccggt gaccaaatgg gcgacgcggg
     1081 aagagcgcga agggcagctg catctctggt ttcactgcgt ggggatacgt gtcagcgatc
     1141 agctggaaag gctgctgtgg cgcagtattc cgcacattat tgttacctcc gcaaccttgc
     1201 gttcgctgaa tagtttttcg cgtttgcagg agatgagtgg tctgaaagag aaagcgggcg
     1261 accgttttgt ggcgctggat tcccccttta accactgcga acagggcaaa attgttattc
     1321 cccggatgcg cgttgagcct tccatcgaca acgaagagca gcatattgcc gaaatggcgg
     1381 cctttttccg taagcaggtg gagagcaaaa aacatctcgg tatgttggta ctgtttgcca
     1441 gcggacgggc gatgcagcgc tttctcgact atgtgacgga tttacgcctg atgctgctgg
     1501 tgcagggcga tcagccgcgt tatcgcttag ttgaactgca ccgcaaacgc gttgccaacg
     1561 gtgagcgtag tgtgctggtg ggcttacagt catttgccga agggcttgat ttgaaaggtg
     1621 atctgctcag ccaggtgcat atccacaaaa tcgcttttcc gcccatcgac agcccggtgg
     1681 tgatcaccga aggggaatgg ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc
     1741 tgccgagcgc ctcgtttaac ctgattcagc aggttgggcg actgattcga agccacggtt
     1801 gctggggcga agtggttatc tacgacaaac gcttgctgac caaaaattat ggcaagcgac
     1861 tactggatgc attaccggta tttccgatag agcaaccgga agtccctgaa ggtatagtta
     1921 aaaagaaaga aaaaacgaaa tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta
     1981 aggagtcgta aatggactat cgcaaaatca ttaaagagat cgggcgcggg aaaaaccacg
     2041 cgcgcgattt agaccgggat actgcgcgcg gtctgtatgc ccatatgctc aatggtgaag
     2101 tccctgacct tgagttgggc ggcgtattga ttgcgctacg tatcaaaggg gaaggggaag
     2161 cagagatgct cggcttttac gaagccatgc aaaatcacac catcaagctg acgccgccag
     2221 caggcaagcc gatgccgatt gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc
     2281 tgacgccgtt gttggcgatt ctcctgcaca aactcggttt tcctgtggtg gttcacgggg
     2341 ttagcgaaga tccaacccgc gtgctgactg aaaccatttt tgaattgatg ggtattacgc
     2401 caacgttgca cggcgggcag gcgcaggcga agctcgacga acatcaaccg gtgtttatgc
     2461 cagtcggcgc gttttgcccg ccgctggaaa aacaactggc gatgcgctgg cggatgggcg
     2521 tgcgtaacag tgcacatacc ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcgc
     2581 tgcgtctttc cagcgtttcg catccggaat acattggacg cgtcgcgaag ttctttagcg
     2641 atatcggcgg gcgggcgcta ctaatgcatg gtactgaagg tgaagtgtat gctaatccgc
     2701 agcgctgccc gcagatcaat ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac
     2761 aggaccctgc tggtagcgag ttactgccac aagcaaaaga tccggaaacc acggcgcagt
     2821 ggattgagcg ttgccttgct ggcagcgaac cgattcccga atcgctgaaa atccagatgg
     2881 cttgctgcct ggtggctacg ggtgaagcgg caactatcag cgacggcctg gcgcgcgtta
     2941 atcaggcatt ttaattcttt tctcagccgg atgagccatg tcatccggct tttaccccac
     3001 cgcaatatga aattcctgca tctttattga ccttcccgcg cccggcgtgc agcataaaaa
     3061 aacaacaaac acataacata aacagcaact atatttaacc tcattggttg tttttttgaa
     3121 caataatatg agtcaagtaa tttcatcatt gtggtgctgt atatggtgcc tgattttatt
     3181 tagcaactct tctaagtttc ttgatcttag caaggtaata ccagtgaact taaagatcat
     3241 gtttactttt tgatctctat caactctatc aggaagatcg tgtgaatagt catcaagttc
     3301 aattgcacat atgattgaaa aatcattttt attaacaatc aaaaaatcta tatgatattg
     3361 agatatagac ctaaatgctt ttataaatga attatattga gtgttcttgt caaatatagg
     3421 aacgataatg tctaccattc taacctgtga aaacacaaaa aagtcctgat gaaattcatc
     3481 ataaatcaat ttaaaaaagc gacgttcgcg attgctcatg aaatttttct tttgatagaa
     3541 ctggaatgca tcatactgaa gtgtatgaga tttctttgat gttgctttat aattaaagag
     3601 aggaggggca taatttggtg tgttatctgg agcatggtgt aacgtcttat gtatactttc
     3661 tttttttagt tttttatctg tgagtcctcg tattaatgaa tataatgcca aaaataaaag
     3721 atagaaaaaa aatagagcta taactgaaag taacagggca agaggtattt gccataaggg
     3781 gattttggga aacataaaac gatcctacaa atgaaagggg cgatattata tggtaaaaaa
     3841 acgaagatta atacaatgaa tcggtaagat tttaaaagat atcaacgtta atacactgta
     3901 tttagatata gtattatgaa cagtttatat aaaaggatta taagaaggtc gcgacaggaa
     3961 aatatcctaa ggcgacctta gagtgaggag acagacatca tttgatgtga ttatccggct
     4021 taacagctca gacttttttc acctgcaaca ggatcaccat atccgtgcgg ctcttctcgt
     4081 caga
//
LOCUS       DAHBHC010000193         4016 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHBHC010000193 DAHBHC010000000
VERSION     DAHBHC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.197"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..739
                     /locus_tag="M2A35_005104"
     CDS             <1..739
                     /locus_tag="M2A35_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7201262.1"
                     /translation="LPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSKAPLGL
                     ALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFADYSRSA
                     ASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTACRGCK
                     GKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQWYKGY
                     ADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(1017..1106)
                     /locus_tag="M2A35_005105"
     CDS             complement(1017..1106)
                     /locus_tag="M2A35_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201263.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(1161..1373)
                     /gene="cspF"
                     /locus_tag="M2A35_005106"
     CDS             complement(1161..1373)
                     /gene="cspF"
                     /locus_tag="M2A35_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HCB7201264.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            1674..1889
                     /gene="cspB"
                     /locus_tag="M2A35_005107"
     CDS             1674..1889
                     /gene="cspB"
                     /locus_tag="M2A35_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HCB7201265.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            2643..2858
                     /gene="essD"
                     /locus_tag="M2A35_005108"
     CDS             2643..2858
                     /gene="essD"
                     /locus_tag="M2A35_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCB7201266.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            2863..3174
                     /locus_tag="M2A35_005109"
     CDS             2863..3174
                     /locus_tag="M2A35_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCB7201267.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            3171..3704
                     /locus_tag="M2A35_005110"
     CDS             3171..3704
                     /locus_tag="M2A35_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7201268.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWGERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            3701..>4016
                     /locus_tag="M2A35_005111"
     CDS             3701..>4016
                     /locus_tag="M2A35_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HCB7201269.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTA"
BASE COUNT         1166 a          750 c          981 g         1119 t
ORIGIN      
        1 cttaccaaaa tattactccc caaaatctcc aaaattgagc gatgacgctc cagcgacagg
       61 caccggttgt ttaacaatta cggatgtaat ggcagcgcag gggatggtgc agtcgaaagc
      121 accacttggg ttggccttat ttctggcaaa agttggtgtt caggaccctc agtttgcgat
      181 tgaaggcctg ctaaattacg cgatggcact ggataacccg acattgaaca aattgagtga
      241 agaaatccgg ttacagatta ttccttacct cgtgagtttt gcctttgctg attactccag
      301 gtctgcggca agtaaggctc gctgtgagca ttgttcaggt acgggatttt ataatgtatt
      361 gcgcgaagtg gtgaaacact acagacgcgg ggaatctgta atcaaggaag aatgggtgaa
      421 ggaactatgt cagcattgcc atggtaaggg cgaagccagc acagcgtgca gagggtgtaa
      481 gggtaaaggg attgttctgg atgaaaaaag aacccggttt catggcgtac cggtatataa
      541 gatttgtggg cgttgtaatg gaaaccggtt tagtcgttta ccgaccacgc tggcacgacg
      601 tcatgtccag aagctggtac cagacctgac cgattatcag tggtataagg ggtatgcgga
      661 cgtcattggt aaactggtaa caaagtgctg gcaggaagaa gcatacgcgg aagcgcaatt
      721 gaggaaggtg acgagataaa tgatttttgc tgaagatggc gacatgatgt ttgcattttt
      781 caaaaaatat ggataaaatt ttttcaacga tgggctttgt atacccgacg ttaagaaaaa
      841 gtagaaaacc cgctgatgag cgggttttgt gctttaaatg gggcaatggt aatgttgaat
      901 ctcatcccgg gactcatgtc tgttaactta ttatttagcg ggtgacttgg ttatttgcct
      961 gatgtttaaa atgttttctt ccagtacaat gtccctaaac acaatgagtc tgcttattat
     1021 attattagca gagctattac ggccaaagta cagcataagc ttttaaagcc aatcaaccag
     1081 tcatcaagac agacggggtt attcataaaa actctccatg tgtgatccga tggggcctga
     1141 aattaaagct ttaatatagc tcatgaaagg taaacattgg cagctgaagg gccacgcaga
     1201 ccatttatcc ggcaaaattc cacgcgtaat ccggtggtaa tttcttctgc atcgcggaga
     1261 ttgagcgctg aaacatgaag ctggacatcg atacgaccat cggatggggt gataagaccc
     1321 ttgccgcttt tgccgtcaaa ggttttgaca attcctgtca ttttacggga caaaaaaatt
     1381 ccttaatact gataacttgg cgcactatac acacgttcct gaagaaagct atagtttttt
     1441 tatggggttg aagatggctg gatgtctaaa ataaacattg cttcatatgt tcaactatgc
     1501 gttaatgatt gcgtcggttt gaagaacaga cgatatacga agtagtttac taaagcagtt
     1561 ctcatttcag gtgttattca cttattcctt ctttgagtct ctccaattaa gtacgaagtc
     1621 gtttctgtta tgcaaaccat ttatgccgaa aggctcaagt taaggaatgt agaatgtcaa
     1681 ataaaatgac tggtttagta aaatggttta acgctgataa aggtttcggc tttatttctc
     1741 ctgttgatgg tagtaaagat gtgtttgtgc atttttctgc gattcagaat gataattatc
     1801 gaaccttatt tgaaggtcaa aaggttacct tctctataga gagtggtgct aaaggtcctg
     1861 cagcagcaaa tgtcattatt actgattaaa attcatcgct cgtctgtata cgataacgaa
     1921 gaaggctgat gcctgagtag agatacggac agagtagtga atattggatc tctttaataa
     1981 aaagtaagga ggtccaatac atgaaacaat ggctagcata tttggcaaaa tcttaatcag
     2041 gaaaagtatg ctaaccattg tggtgaagtg caggtttgct gcatgaatag ttttacagca
     2101 gaagctaact gctggcatgg caaaacaaag tgcgtaagtg gatgactccc acaaaaagca
     2161 ccacaatctc aaacccgctc aggcgggttt tttattatct gctttaaata tattattaaa
     2221 atataaaaaa tacttgttac taataaaatc aatcaggcta cagctttaag atttgtctgg
     2281 aatactttgt tgcaatgagg gcagatcaaa agggcacctt tttgtactct tgaaaaactg
     2341 tgttctgact cttgggtgca gtttgggcag gaacatttaa cgagataatt acggcgtgat
     2401 tttgagtttt tacgttctga cataggcttt tcctgtataa atggccgtat acagtacact
     2461 aaatatgaaa acatttctcg tattattatt ttatatatga ctttctttca aaataattac
     2521 ccacattttt aatgtgtatg tttttttagc gccgttgaga acaacgtgtg ctgtcaaaac
     2581 taccccgtag actccgatct tttcaaacat attgcaccat ccgtgtacat cggggtgagg
     2641 atatgaaatc aatggataag ttaacaacag gtgttgccta tggcacatcg gcgggtaatg
     2701 ctggtttctg ggcattgcag ttactcgata aagtaactcc gtcacagtgg gctgcaatcg
     2761 gtgtgctggg tagcctggtt tttggcctgc tgacgtatct gacaaatctt tatttcaaga
     2821 ttaaagaaga caggcgtaag gctgcgagag gagagtaatc caatgactca agactatgaa
     2881 ctggttgtga aaggagtccg taattttgag aataaagtta cggtaactgt agccttacag
     2941 gacaaagaac gctttgacgg tgaaattttt gacctggatg tcgccatgga ccgtgttgaa
     3001 ggagctgcgc tggagtttta tgaggcagca gccagaagga gcgtccggca agtcttcctg
     3061 gaagtagcag aaaaattgtc agaaaaagtt gagtcttatc tgcagcatca gtactccttt
     3121 aagattgaaa atcctgccaa taagcacgag cgtcctcatc ataaatatct atgaacacaa
     3181 aaatcagata cggcctgtcg gctgccgttc tggcgctgat tggtgctggc gcatctgctc
     3241 ctcagatact tgaccagttt ctggacgaaa aagaaggtaa ccacacaatg gcataccgcg
     3301 atggttctgg catatggacc atctgtcggg gtgccacagt ggtggatgga aaaaccgttt
     3361 ttcccaatat gaaactgtcg aaggaaaaat gcgaccaggt caacgccatt gagcgtgata
     3421 aggcgctggc atggggggag cgcaatatta aagtaccact gaccgaacca caaaaagcgg
     3481 gtatcgcgtc attttgtccc tataacattg gccccggtaa gtgtttcccg tcgacgtttt
     3541 ataagcggct gaatgctggt gatcgtaaag gtgcatgcga agcgattcgc tggtggatta
     3601 aggatggcgg acgcgattgc cgcattcgtt caaataactg ttacggtcag gttattcgtc
     3661 gtgaccagga gagcgcatta acctgctggg ggatagaaca gtgaatcaga tattcatggt
     3721 gatttttctc gtgttgtcag gatttatcgt cggaaatgtc tggagcgacc gaggatggca
     3781 aaaaaaatgg gcggaacgtg atgctgccgc attatcacaa gaggtaaatg ctcaatttgc
     3841 tgctcgaata attgaacagg ggcgaactat agcccgtgat gaggctgtta aagatgcgca
     3901 acagaaatct gctgaaattt ctgccagggc tgcttatctg tctgatagtg ttaaccagtt
     3961 gcgtgccgaa gcaaaaaaat atgccatacg ccttgacgca gcgaagcata ccgcag
//
LOCUS       DAHBHC010000194         4002 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHBHC010000194 DAHBHC010000000
VERSION     DAHBHC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.198"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(151..699)
                     /gene="pgsA"
                     /locus_tag="M2A35_005112"
     CDS             complement(151..699)
                     /gene="pgsA"
                     /locus_tag="M2A35_005112"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCB7201270.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="M2A35_005113"
     CDS             complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="M2A35_005113"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCB7201271.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="M2A35_005114"
     CDS             complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="M2A35_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCB7201272.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQVGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            3537..3713
                     /locus_tag="M2A35_005115"
     CDS             3537..3713
                     /locus_tag="M2A35_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201273.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3700..3924
                     /locus_tag="M2A35_005116"
     CDS             3700..3924
                     /locus_tag="M2A35_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCB7201274.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
BASE COUNT         1046 a         1033 c          925 g          998 t
ORIGIN      
        1 ttcatcaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
       61 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
      121 gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc
      181 gctcaaatat tgcaacattg accacagagt cagtaccgca gccacaaaga aaagtgcaat
      241 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg
      301 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
      361 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg
      421 tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag
      481 aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
      541 tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc
      601 aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac
      661 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
      721 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
      781 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
      841 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
      901 ccttcaatgg tttccaggga gctggtattt ttgaccttcg cccgtttttt acggtgcccg
      961 ccaatcgcgt gatcgtgtga ttcatcgcga atatgctgga taacatgcag cgcgggagaa
     1021 tctggcggca aactgaatcc ctcaccttcc ggctcaaaga acagtgtttc cagcccagcc
     1081 ttacgatcta ctcctttggc aacgccaagt agcagtggat gatttttatc ccatgagaca
     1141 tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg
     1201 atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga
     1261 ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc
     1321 gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg
     1381 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
     1441 agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
     1501 gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga
     1561 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
     1621 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
     1681 aactggccta cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg
     1741 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
     1801 atgcccgcat cgaacgccac accaataacg tcgaggtcgt cgccggtatt ggaaacgaat
     1861 tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgagc agcttcttca
     1921 aactccagat tctggctggc agtttccata cgactaatga gttgcgtaag cacctgatca
     1981 tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc
     2041 accagtcctt caacgcacgg tcccagacag cgccctatct ggtattgcag acacggacgc
     2101 gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc
     2161 gccagtgttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
     2221 tgcttcgcac cacgatgcat cgccagacgc gggtgggtat caccactcag gaagataaaa
     2281 ggatatgatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag
     2341 ttgtgttcca gcaacagcgc ttcggtttct gtgtgagtaa ccgttacatc aatttgctgg
     2401 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
     2461 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
     2521 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
     2581 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
     2641 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
     2701 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
     2761 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
     2821 ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc
     2881 ctgaatagac agaacgaatc gcactcacaa cttcctgcgg agccgcgcct ttgctgaggt
     2941 agcccgcagc accgacctgc atgactttcg ctggtaaagg gttttctgta tggacggtaa
     3001 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
     3061 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact
     3121 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
     3181 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
     3241 aaggaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
     3301 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
     3361 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt
     3421 cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
     3481 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa acattcatga
     3541 taaaaatatt tattggtcat tatattaacg tattttatag cactgccgat atcacgctca
     3601 aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc ttaacatttt
     3661 tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc tgatttttct
     3721 actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc gatgctgacg
     3781 ctgatgctgc aggcgatggg acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa
     3841 cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac ggttaagcaa
     3901 attaaacaag cctaccgtca gtaatataaa accggctgag agcgtgcctt tcagccggtt
     3961 tttgcatctg gcacgcagaa agaaaagcga tcaaattaag cc
//
LOCUS       DAHBHC010000195         3973 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHBHC010000195 DAHBHC010000000
VERSION     DAHBHC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.199"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..260)
                     /locus_tag="M2A35_005117"
     CDS             complement(<1..260)
                     /locus_tag="M2A35_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7201275.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAA"
     gene            complement(250..1191)
                     /locus_tag="M2A35_005118"
     CDS             complement(250..1191)
                     /locus_tag="M2A35_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCB7201276.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMACYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(1192..2205)
                     /locus_tag="M2A35_005119"
     CDS             complement(1192..2205)
                     /locus_tag="M2A35_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCB7201277.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(2223..3368)
                     /locus_tag="M2A35_005120"
     CDS             complement(2223..3368)
                     /locus_tag="M2A35_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCB7201278.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(3613..>3973)
                     /locus_tag="M2A35_005121"
     CDS             complement(3613..>3973)
                     /locus_tag="M2A35_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCB7201279.1"
                     /translation="ALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKIH
                     HNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWGE
                     REEQAVKQLGKLIQERL"
BASE COUNT         1001 a         1113 c          985 g          874 t
ORIGIN      
        1 gctgccagcg tccctaacac cagcgccatt aatgtcgcca gcgccgccac tttaagtgac
       61 agtgtcacgg catcaagaat atcactacgc tgtgctgcca cgctaaacca gcgcagcgtc
      121 aggccctgcg gtggaaaact aaacgccgca tcttcagtgt taaacgcata agcggcgata
      181 atcaggatgg gaaaatgtag gaaaaccacg ccgccccagg ccgccagttt gaggaaaaac
      241 ggtgcgcgtt cagagtgcat cgaacgctcc cagacgtttc acgaacgcca ggtacagtgc
      301 gatgagaaca atcggcacca gggtgaatgc cgccgccatc ggcatattgc caatcgcccc
      361 ctgctgggaa taaaccatat tgccgataaa atatcctgga ggaccaacca gctgcgggac
      421 gataaaatcg cccagtgtga gtgagaaggt aaagatagag ccagcggcaa tacccgggat
      481 tgccagcggc agcaccacat agcgaaaggt ttgtcgtgga cgtgcgccga gatcagccga
      541 cgcctgcaac aatgacggcg gcaaacgctc aagcgccgcc tgaacgggca ggatcatgaa
      601 cggcaaccag atatagagaa acaccagaaa gcgccccagc ccggaagttg acagcgtatt
      661 tccgcccacc gcaggtaatg taaggaacgc agtcagcagt ggttccagcc caagatgttg
      721 taaaaaccac tgagccacgc catcttttgc cagcaataac gtccaggcat aggctttaac
      781 aatgtagctc gcccacatcg gcaacattac cgcaatataa aaaaaagctt tcattttccc
      841 gctggtatag cacgccatat accacgccat tggaaaagcc agaatggcgc tggcgatagt
      901 gactgccaca gccatagtca gcgtgcggag aatgatgtcg taattcgccg gattaaacag
      961 cgcacggata ttcgccagcg tcagttccgg cgttaccgac atggtgaaat cgtcaaaagt
     1021 ataaaatccc tgccataaca gtgtcagcag cgagccgaaa tagacaatgc caaaccacat
     1081 tagcgggcca agcagcagta aaaacagccc cagccccgga ttacgccaga agaaaccgga
     1141 caccttaccc agacctggac gtgaaggtga ttgcaataca ttcatcgcca ttcacctctc
     1201 ctcaaccagc ggcaccatca catcacgcga ccaggaaacc atcacctgtt gtccgggcgt
     1261 gagcgtggca ggcagttctt cgcctgtcat attggcctga ctcacaagca gtttttcacc
     1321 gccgttcaat ttcagttcaa aacgagttgc cgcgccctga tattgcaccg cctggatcgt
     1381 gccattggcc tgcagttcac caggggtgtt gaggcggata tgttccggtc gcagggcgaa
     1441 gcttcccgtc atgccacaaa gtttctctgc catcagtcca tcaaaaacgt tggatgtccc
     1501 gacgaatccg gcaacaaacg gcgtgcgcgg gcgcatatag agatcgcgcg gggaatcgac
     1561 ctgctcaatg cgtccattat tgaaaaccgc cacacgatcg gacatcgata acgcttcgcc
     1621 ctgatcgtgg gtaacgaaga taaaagtgat accgagagac tgttgcagtt ttttcagttc
     1681 cagctgcatc tgctcacgca atttgagatc cagtgcgccg agcggttcat ccaacagcaa
     1741 tacgcgcggt tcattcacca atgctctggc gatagcaacc cgctggcgct gaccaccaga
     1801 aagttgtgac ggtttacgtt gatgtacaaa ccccaacgcc actttctcca gcgcctcttg
     1861 cgccattgcg tgccgctgct ttttattcac gcctttgacc atcagcccat aggcgacatt
     1921 gtcaagaatc gacatatgcg gaaatagcgc gtagtcctga aagacagtat tcacgtcccg
     1981 ctcccacggt ggcagattgc tggcgggttt accaaagata gagatagccc cgccggaaag
     2041 ctgttcgaag ccagcaatca ggcgcaggca ggtggttttg ccggagccgg acggccccag
     2101 catagagaag aactcaccat cttttatcgc aatactgacg ccatctactg cgcgcacgtc
     2161 accgtacaac cgcgagacgt tgtcaaactc cactgcgtac gtcataaagc accccagcga
     2221 agttagcgac cgcccataat ggcaatgtaa tcctgcgtcc agcgactgta gggaacaaac
     2281 ttgccccctt ctgctatagg cgttttccag aaggcgattt tgtcgaaata gttaaaaccg
     2341 ttggtttcac aacctttttc gcctaataac ggactggctt tacacccttc cggcactacc
     2401 ggtaacgagc caaaccaggc cgccacatcg ccctgcactt ttggcgttaa tgaccagttc
     2461 atccatttgt aggcgcaaac cggatgtttc gcttcgctat gcagcatggt ggtatcagcc
     2521 caaccggtaa caccctcctt cgggaaaacg gtagcaacag gctggccttc ggctttcagg
     2581 gcgttggcct gatagggcca ggcactggaa gcaaccacac cctcgttttt gaaatcgctc
     2641 atttgcacgg tagtgtcatg ccagtagcga tggatcaaac tgtgttgagc gcgcagcact
     2701 ttcagcaccg cctggtactg ttcttcggtg agttgatacg gatcgctgat gcccaactgc
     2761 ggctgagtgg ctttaacgaa caacgcagcg tccgcaatgt agatagggcc atcataagcc
     2821 tgaacgcggc ctttattgct cttgccgtcc ggcagatttt gctcaacaaa aaccacttgc
     2881 cagctatccg gcggcgtcgg gaaggtttta gtgttgtaca tcagcaggtt cggcccccat
     2941 tggtaaggtg tgccgtaaac tttgccgcca acattaaacc agtcgccttt aaccacgcgc
     3001 ggatcgagcg ttttccagtt gggaatcaat gcggtattaa tcggctgcac gcgtttaccc
     3061 ataatcaaac gcagcgaggc atcgccggat gccgtaacca gatcgtaacc ccctttggtc
     3121 atcagactga ccatttcatc ggaagtcgcg gcggttttca cattcaccgc gcagcctgtc
     3181 tctttttcga actgcgttac ccagtcgtat tgtttatcag tttgtccgcg ttcgatgtat
     3241 cccggccagg cgataatatc cagtcgccct tccggtttat ctaaattggt aggcggttcg
     3301 gcggcgtgag cggtcattat tgtcatgctg agcgcacaca ggctgctgcg ggcaaatgtc
     3361 ttgctcataa ggtcttactc ctgtcgtaat taattgttca gccgccgtgc cgtaaaaata
     3421 tctccttgtt taaccataga cggcggcttg caggcacacc ttcgcccctg agaaaattta
     3481 atcaggttgt gagctaccgc gcattatgcc gtctgtaagg ttatcttctg gagtatagtc
     3541 aaggagttag gaaatatgac tgctattcga aattcatatt acccaatcta agaggaatta
     3601 aattaaacgc tattacagcc gttcttgaat aagtttgcct aattgtttta ccgcctgttc
     3661 ttcacgctct ccccactgcc acgcggtatt aaaacggaaa aaacgtgacc agttttcacc
     3721 ggtagaaaac attttacccg gcgcaatact gatatgatgc gtcagtgccg ccaggcttaa
     3781 ttcgccggca tctaacggct cggggagctc cagccagaga aagtaaccac tgtcattatg
     3841 atgaattttc acttccgcag gcagataacg cagcagtgcc tgccaggcac gttgtttacg
     3901 ttccgcaagc tggcgacgca ggcgacgaag atgggcgtcg tatcggcgcg tggaaaggta
     3961 atccaccagc gca
//
LOCUS       DAHBHC010000196         3816 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHBHC010000196 DAHBHC010000000
VERSION     DAHBHC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.200"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1136
                     /locus_tag="M2A35_005122"
     CDS             <1..1136
                     /locus_tag="M2A35_005122"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCB7201280.1"
                     /translation="NDEIPAIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGV
                     VASIAPWNYPLMMAAWKLAPALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINV
                     LFGRGKTVGDPLTGHPKVRMVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFD
                     DADIEAVVEGVRTFGYYNAGQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDE
                     STELGPLSSLAHLERVSKAVEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDA
                     IVQKEVFGPVVSVTPFDNEEQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTW
                     VNTHFMLVSEMPHGGQKLSGYGKDMSLYGLEDYTVVRHVMVKH"
     gene            1346..2155
                     /locus_tag="M2A35_005123"
     CDS             1346..2155
                     /locus_tag="M2A35_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCB7201281.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
     gene            complement(2557..2652)
                     /gene="yncL"
                     /locus_tag="M2A35_005124"
     CDS             complement(2557..2652)
                     /gene="yncL"
                     /locus_tag="M2A35_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCB7201282.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            2848..3021
                     /gene="ortT"
                     /locus_tag="M2A35_005125"
     CDS             2848..3021
                     /gene="ortT"
                     /locus_tag="M2A35_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCB7201283.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            3107..3340
                     /locus_tag="M2A35_005126"
     CDS             3107..3340
                     /locus_tag="M2A35_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCB7201284.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(3341..3790)
                     /locus_tag="M2A35_005127"
     CDS             complement(3341..3790)
                     /locus_tag="M2A35_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCB7201285.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
BASE COUNT         1096 a          763 c          934 g         1023 t
ORIGIN      
        1 tcaatgatga aatcccggcg attgtcgatg tttttcgctt tttcgcgggt gcggcgcgct
       61 gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca tacttcgatg atccgtcgcg
      121 atccgttggg ggtcgtggct tctatcgcac cgtggaatta tccgctgatg atggcagcgt
      181 ggaaactggc tccggcgctg gcggcaggga actgcgtagt gcttaaacca tcagaaatta
      241 ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga tatcttcccg gcaggcgtga
      301 ttaacgtact gtttggcaga ggcaaaacgg tgggtgatcc gctgaccggt catcccaaag
      361 tgcggatggt gtcgctgacg ggctctatcg ccaccggcga gcacatcatc agccataccg
      421 cgtcgtccat taagcgtact catatggaac ttggtggcaa agcgccagtg attgtttttg
      481 atgatgcgga tattgaagca gtggtcgaag gtgtacgtac atttggctat tacaatgctg
      541 gacaggattg tactgcggct tgtcggatct acgcgcaaaa aggcatttac gatacgctgg
      601 tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg tgcgccggat gacgagtcta
      661 cggagcttgg acctttaagc tcgctggcgc atctcgaacg cgtcagcaag gcagtagaag
      721 aggcgaaagc gacagggcac atcaaagtga tcactggcgg tgaaaagcgc aagggtaatg
      781 gctattacta tgcgccgacg ctgctggctg gcgcattaca ggacgatgcc atcgtgcaaa
      841 aagaggtatt tggtccagta gtgagtgtta cgcccttcga caacgaagaa caggtggtga
      901 actgggcgaa tgacagccag tacggacttg catcttcggt atggacgaaa gatgtgggca
      961 gggcgcatcg cgtcagcgca cggctgcaat atggttgtac ctgggtcaat acccatttca
     1021 tgctggtaag tgaaatgccg cacggtgggc agaaactttc tggttacggc aaggatatgt
     1081 cactttatgg gctggaggat tacaccgtcg tccgccacgt catggttaaa cattaagtta
     1141 gatgttagtg gctgttatag gacggccact atcgttttag gttctgagtt taatggcggt
     1201 taagtattag aatgattaca ttaataacta gcgtaaaaat taggctaaat gtttgatggc
     1261 gatttttcat cctgtcacag ttgattagca taaggcttta gagtatcgtt attaaactaa
     1321 taatttataa gtagaagtat taacaatgaa agttcaattg tacaaattca cggaagataa
     1381 aaataaaacc cttaccttca gatggacgaa aaaacacttt gagttttgta tggataataa
     1441 aatcttttta aatcataagg ggaagaagtc gtataaagaa aggaatttat ttcttttctc
     1501 aaaaggtgac aagataacaa ttgaagataa tgttattgct gaagaatatt caacaatgcc
     1561 tgtgaaaaat ttctccagtg tcggcgcttt ttcatttccg acctgccatt tttcagggaa
     1621 tatacgtata ggtcgatttt gtagtattgc atcaaatgta aaaataatgg gtggaaatca
     1681 tcctttgaat cgttttacta ctcacatgat gacttataac ggtgaatttg ataagtttgc
     1741 tatgagtgaa tttgaacgta gctggacatt gaaaccattc attaccaaac ctgaaaatcc
     1801 gattatagga aatgatgtct ggataggaaa tgatgttgta ttgaaaggtg gcatcgcaat
     1861 aggtgatggt gctgttattg ctgccaattc cgttgttacc aaagatgtgc ccccatatgc
     1921 catagttgca ggagttcctg caaaaataat cagatttagg tttgattcaa atgttattga
     1981 tgaactgttg agaataaaat ggtggaatta taactactca gatcttccgg ataataacaa
     2041 gtgtgatgat ataaattatt ttgttgaaga gatgaatcga ttgatatcag atggaaatat
     2101 tcaagagagg gactataaaa aattcaatct ttctgaggta ttcagaggcc tataattatc
     2161 tgttttgcat gataaatggg caagataacc agggcggggt ttgcaatttc aatggggcag
     2221 cgtcatcaag gaggttgcaa acatcctgcc atgcatatac tttacgccat ctttttatat
     2281 ttacgttatt ccttgaaagt ataaagcatt tatagactcc cgcgcgtata aagtattcat
     2341 gatagaaaaa tgtgaaaaca agattccgtt tgaatcagta agctgtttgt atagacgaca
     2401 gtagggggat gttttctact catttcaatg tagatgccag caaaaagaag taaatattaa
     2461 ggataatgtt gtaaacctgg agagtatgag aagatcttgc gtactgtcag agagttgatt
     2521 tctctgacag tacgtgctgc gtgcaaaatc actacatcaa ataaaccagc caaatctcat
     2581 agagataaga gtaaacaaac caacagcagc aaagacattt atcagtacta cagttctact
     2641 ggaaacattc atttttgcca ccttttatca ttgcccctat gtgtatagta cgggttaaga
     2701 aaatccgcaa taatactcat tctgaagatg gataagggca agttgctgtt tgatgatttt
     2761 ttctcaaact ggtgaaaaaa acttgatgca cgtcaaaaaa tgacgcatat ttgcgcgcgt
     2821 tttattcatc tggctggacg cccgtacatg tctctctatc aacatatgct tgttttttat
     2881 gcggttatgg cagcaatcgc atttcttatc acctggtttc tttctcacga taagaaacgc
     2941 atccgtttct taagcgcctt tctggtgggg gcaacatggc cgatgagttt cccggtggcg
     3001 ctgttgtttt cactgtttta aatcttactc actggcttga cggcaccacg cagacttata
     3061 tcatttggat gaatcgataa atttcacaag tggttaagga gaaagtatgt cgcatctgga
     3121 tgaagtcatc gcgcgcgtgg atgccgcgat tgaagagagc gtcattgccc atatgaacga
     3181 attattaatc gccctgagcg atgacgcgga gttaagtcgg gaagatcgct acacccagca
     3241 gcagcgtttg cgcaccgcta ttgctcatca cggtcgcaag cataaagaag atatggaagc
     3301 gcgccacgaa cagttaacca aaggcggcac catcctctga ttaaaatgag cgtctggcta
     3361 ccaaccaggc gccaaccaca agtaaaattg cgccacaaat tgggccgaac aacgcccgta
     3421 gtggtacgcc atgactgcgg aagatatcca gcatcagacc gccaattagc tggctggcga
     3481 ccagtactgc aatagttgtc gccgctccca cattctgata tccgctgata ctggcgaaga
     3541 caaaaaacga tcccagcaag ccgggtataa gtgtccacca gcgcacgctg gacaccagtt
     3601 cgccaaaccc cgccatgcct tgtttaaacc ataaaataga aacaaagagc acaatcccca
     3661 ccagtgagtt cagcaacatg gcgatgagaa tggtagagga ggtctgggtg atgcgcacca
     3721 tcagcgtgtt ctggactacc agaccaattc cggcggcaat caaaaaagca agggtgagcg
     3781 actgattcat ccaatcgcgt ccggttcagt gcgttc
//
LOCUS       DAHBHC010000197         3810 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHBHC010000197 DAHBHC010000000
VERSION     DAHBHC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.201"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..434)
                     /locus_tag="M2A35_005128"
     CDS             complement(<1..434)
                     /locus_tag="M2A35_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCB7201286.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLL"
     gene            complement(431..532)
                     /locus_tag="M2A35_005129"
     CDS             complement(431..532)
                     /locus_tag="M2A35_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201287.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            complement(656..1057)
                     /locus_tag="M2A35_005130"
     CDS             complement(656..1057)
                     /locus_tag="M2A35_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201288.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLK
                     GFARVNDGKVRVSNSMVNGLEIVDCLIYELK"
     gene            complement(1036..1452)
                     /locus_tag="M2A35_005131"
     CDS             complement(1036..1452)
                     /locus_tag="M2A35_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HCB7201289.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            complement(1752..2360)
                     /locus_tag="M2A35_005132"
     CDS             complement(1752..2360)
                     /locus_tag="M2A35_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201290.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(2786..2854)
                     /locus_tag="M2A35_005133"
                     /pseudo
     CDS             complement(2786..2854)
                     /locus_tag="M2A35_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3113..3460
                     /locus_tag="M2A35_005134"
     CDS             3113..3460
                     /locus_tag="M2A35_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201291.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(3665..>3810)
                     /locus_tag="M2A35_005135"
     CDS             complement(3665..>3810)
                     /locus_tag="M2A35_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HCB7201292.1"
                     /translation="ARINRGETIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKG
                     ASV"
BASE COUNT         1090 a          841 c          765 g         1114 t
ORIGIN      
        1 agcagctgga actcgcagct ctgcggaata gtcaggtggc agccaatatt catcgcccag
       61 gcttcaacct tacacaggaa gacatacatc tctccggtat caagatcgga ggtatggcgt
      121 aacgactgga tagtagtgat ttcgccggtt acgacatcaa ccaggtcctt ggtttcataa
      181 ccgaggtatg tgtgtttgag agcatctttt acccatgctg cggtagcgaa cgatttcccc
      241 ctgcggataa ggtattcact gatttcgctg taccacatgt ggctaagtgc attctgggaa
      301 agactgcgtc tctcgcgcca cggtttaagc accatgcgaa agcacttgcc gtcttccaaa
      361 taaggctgga gctgctgacc gatagcggtg aagttaccgc gatgcagttt gatgccgtct
      421 tgtgggaggt tcacgcttca cctccgcaga ggtcaaacgc tggataaaga aaatcgcagg
      481 tgcatttctg catctgtgac gggagaagat agtttagatt gtatgtgcgc ataaacgtcc
      541 ccgtttagcg cagaagtcac cggaggagtt caaactccgg tgacttaatt atgacaagtt
      601 gattattgaa aatcaaaagt catttaaaca ataatgatac ccagcaagag atttcttatt
      661 ttaactcata gatcagacag tcaacgattt ctaaaccatt taccatagaa ttagaaacgc
      721 gaactttacc atcgttgact ctagcaaatc cttttaacat tttataaaca aaagctgaat
      781 cccccttagc ctcaattgct tctaatggta tcggcttata cttttttgat atgctatttc
      841 tatttatctc actgattttt ctatatattt ctgccggccc acgttctgag aagtcactga
      901 taactaaaat atatctccaa gatagatctg ggtaataaaa ccacattgca gcatcaatag
      961 ttaaattctg taacttgagt ttttttaaca aaaattgacc tgagaattcc atatcttttg
     1021 tcaactctct gccgattacc agtgtgcttt tacccatttc aaaactccag aattttggtc
     1081 acctacagca tcaaacaatt gttcggccat agctttactt atgttattgt cgtatctaaa
     1141 ttgctcgctc cagtctttaa cgaccgacca gttaatctcc aacgaaacat cattttttgc
     1201 gtcagcaatc aaaatttgat gtaagttggc aattttgaga agttgcgaca aatcatgagt
     1261 atatgaatca tttacgactt ttttgtttgg aaactcatgt tgtaaaaatg acttggcaat
     1321 acaggccttc aatgcacatt caactgcata cccacaaaga taatatgcac catgaaaaaa
     1381 gccatgatca agcaagcatt tggcctcatt aagcctagtg tcagataatg cttcaaggtc
     1441 gtttttattc atacaattat aacttgaatt atcctaattt ttatgattat acccttatca
     1501 ggtatgatgt aggaactgaa aacaatattg gatatcattt tcggtatttc tctgcaaatt
     1561 ccagcccaca ggatctcggt gtacaatctg tcgctgatcg cgcaaattta gggtcaatca
     1621 cttcattttt tgcatcaaac cctcactaac atcgaacgat tatagtacaa gtagtgttct
     1681 tgacttctca ttaaaccgtc agcattcaat taattacaaa atactagcgg catttctctc
     1741 ccgcaacttt attacagccg ttcagaacgt aaatgcgtca atactcgact ccacttatca
     1801 tcctgccacg gccggaattt tacatgtgcc gtttctctgg caaggattgc ctgtgcctta
     1861 ttgagtatcc ggggatattc ttgttcgatt gaagtgaagc gaccggcttc gcggtgttct
     1921 gctacctgaa gaagcggagt gacgttttgg caagcggtta acattgtgtc acttgcccgc
     1981 cataaccaag caagtgtgca aagttcgtta tcagtgaatt gttttgtgat tggggattgt
     2041 tgaatttctc gatcgagaat atccagcacc cagcggcgga attctttggc cacaggagtg
     2101 cgggcgaaca tggcgatcag atgggcgcca cggagggaga aaatgcgagt cttcttacga
     2161 taatttcctg aggtcgttga ttcgacgacc tgagacattc cgtcagtaaa ctcatcaata
     2221 tttttgttgt agatgtcggt tactgctttg gtgctggcat attttaatgc tgccgccaat
     2281 tctttgctgg taaaccagat ctggttgttg tgatttatag gagtgaattt tacatcgcgg
     2341 aatgcgagct gagtgaccat agcgataatc tcgttttgaa tttgagctat caccaccgac
     2401 gacgccaatc gactggtggt ggactgtgca aggttggcgt aaccggtcaa aacgaaatcc
     2461 ggcgcttccg aagaagccct cacacagccc accataattt ttgtgtagac gtgcactgcg
     2521 cataaaaaaa ccgcgaacgc ggttatgcgt cgttttgatg tccgggacgc caatcccgtg
     2581 tgccgatttt gcggcaacgc acagaatata gcgccggata aataatgtcg tcaacccttg
     2641 catatggggg gcgcgatgat cactactacc accataatcg ctccaactgt tgcaactact
     2701 ttaggctaag acatcacgat ttccatatca cctttagccg ccagacaaac agagactccc
     2761 tgttcttggc tgccaaaaac accgaatgca aaaaatcgca ggtgtatttc tgcatctgtg
     2821 gagggagaag agagtttgga ttgtatgtgc gcataaacgt ccccgtttag cgcagaagtc
     2881 accgtagttg ttcatattct gatgacatga taatttccta ttgaatagaa aaaattaaca
     2941 cgactcattc ttatgactgg aaaaagcaat catgctaata atcattgtta ttccagttcc
     3001 aaatgcagcg aaataatcac caatattttt tctaccggtt tcgactttga tgctatattc
     3061 tgctaacacc taactaacac tacgtttcac acagcaatta catggaaata acatgactat
     3121 caaagcgact gacatagaga ttcttcatcg ttatgctgaa ggggtaatgg aacgttctaa
     3181 ccatcatgca aaaaatgttg gtgcagccgc tcttacattg ctaggaggtg ttatctggaa
     3241 agccctgccc ggatcaatcg agataagaac gtataacgga agtcttgcaa atatggtgtg
     3301 gtggcaaagc gaaagaacat taaaaaatta tgcaatctca tacaaccata attcatgtga
     3361 aattgaaatg agagatgaat cagtcaaagg tgctgtatta tttagcattt ccaacgaaac
     3421 cactccagaa aaaatattat cacagctttc tgagctttaa ttaaaggcgg tgctaacacc
     3481 gcccatacca ttactccaca tcttacgcaa cttgataatt atgctctatt aaatacccac
     3541 aatatttcat caatgcatgg gtcagctaca aaatgtttac cgtggtaatt cctgttttcg
     3601 caacatctca aaagaacaat aaacactgta tttcttcaag taacgaagaa tctttctttg
     3661 cccgtcatac acttgctcct ttcagcccaa acttagcttt gatttctgcg atcttcgcca
     3721 gagcctgtgc acgatttaga ggtctaccgc ccataacagg aagttgtttt actggttcag
     3781 gtatcgtctc accacggtta attcgcgctg
//
LOCUS       DAHBHC010000198         3803 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHBHC010000198 DAHBHC010000000
VERSION     DAHBHC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.202"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..266
                     /locus_tag="M2A35_005136"
     CDS             <1..266
                     /locus_tag="M2A35_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCB7201293.1"
                     /translation="DVAKRFDSGVAVGVWAAISNVSKDDYGEGGFSKGFYISIPFDLM
                     TIGPNRNRAVVSWTPLTRDGGQMLSRKYQLYPMTAEREVPVGQ"
     gene            312..1451
                     /locus_tag="M2A35_005137"
     CDS             312..1451
                     /locus_tag="M2A35_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCB7201294.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTANADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKIFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            1439..1885
                     /gene="etp"
                     /locus_tag="M2A35_005138"
                     /gene_synonym="yccY"
     CDS             1439..1885
                     /gene="etp"
                     /locus_tag="M2A35_005138"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCB7201295.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            1905..>3803
                     /gene="etk"
                     /locus_tag="M2A35_005139"
     CDS             1905..>3803
                     /gene="etk"
                     /locus_tag="M2A35_005139"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCB7201296.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQL"
BASE COUNT         1003 a          911 c         1008 g          881 t
ORIGIN      
        1 tcgacgtcgc caaacgcttt gacagcggcg tggcggtagg ggtatgggcg gcaatcagta
       61 acgtatctaa agatgactac ggcgaaggcg gctttagtaa aggtttttat atctcgattc
      121 cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc ggtggtttcg tggacaccat
      181 tgacgcgtga tggtggacaa atgctgtcac gcaaatacca gctctatcca atgacggcag
      241 agcgagaagt accggttgga caataacctg acgtaattca taacattaca ttgactgata
      301 aatataaacc aatgaaaaag aacattttta agttttcggt actgacattg gctgttctgt
      361 ctttgacagc atgtaccctt gttcctggtc agaatttatc gaccagtaac aaggatgtca
      421 ttgaactacc tgacaatcag tatgacctgg acaaaatggt caatatctat cccgtcacgc
      481 caggattgat tgatcagctg cgcgccaaac cgattatgtc gcaggctaac ccggagctgg
      541 agcagcaaat cgcgaactat gaatatcgta tcggcattgg tgatgtgttg atggttacgg
      601 tctgggatca cccggaacta accacccccg cagggcaata ccgtagcgcc agtgataccg
      661 gcaactgggt taacgccgat ggtgcgattt tctaccccta tattggtcgc ctgaaagtgg
      721 ccgggaaaac acttacccag gtacgcaacg agattacagc gcgtctcgat tcagtcattg
      781 aaagcccgca ggtcgatgtc agcgtcgcag cgttccgatc gcaaaaagcg tacgtcaccg
      841 gcgaagtcag caaatcgggt cagcagccga ttaccaatat tcctctgacc atcatggatg
      901 cgattaacgc cgcgggcggt ctgaccgcaa atgcagactg gcgcaatgtg gtgctcaccc
      961 agaatggcgt aaagaccaaa gtgaacctct atgcgctgat gcagcgtggc gacttgcgcc
     1021 agaacaaact gctgcacccg ggcgatattt tatttattcc gcgcaacgat gatctgaaaa
     1081 tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa aatggaccgc agcggtatga
     1141 ccctggcgga agcgctgggt aacgctgagg gtatgaatca ggatgtcgct gatgcgacgg
     1201 ggatttttgt tattcgtgca acgcaaaaca agcagaatgg aaaaatcgcc aatatttatc
     1261 agctgaatgc gaaggacgca tcggcaatga tactcggaac ggaattccag cttgaacctt
     1321 acgacattgt ttatgtcact accgcgccgc tggctcgttg gaaccgtgtg atttctctgc
     1381 tggtaccgac gatttccggg gtgcatgacc tgacagagac ttcacggtgg attcaaacat
     1441 ggcccaacta aaatttaact caatcctggt ggtttgtacc ggcaacattt gccgttcgcc
     1501 cattggcgag cgcttgctgc gtaaacgtct gccgggcgta aaagtcaaat cggcaggtgt
     1561 tcatggtctg gtaaaacacc ctgcagatgc gacagcggca gatgttgccg ctaatcatgg
     1621 cgtatctctt gaaggacatg ccggacgtaa gctcactgct gagatggcac gaaattacga
     1681 tctgattctg gcaatggagt cggaacatat tgctcaggtt accgcaatcg ctcccgaggt
     1741 ccgcgggaag acaatgctgt ttgggcaatg gctggaacag aaagagatcc cggatcccta
     1801 tcgtaaaagt caggacgcat ttgaacatgt ctacggtatg ttggagcgcg ccagtcagga
     1861 atgggcgaag cgcctcagcc ggtaatttga gtttataaaa tacgatgaca actaaaaata
     1921 tgaatacgcc accaggcagc actcaggaaa atgagatcga tctgcttcgt ctggtcggcg
     1981 agttatggga tcaccgtaag tttattatca gcgtgaccgc gttattcacg ctgatcgctg
     2041 tcgcttactc gctgttaagc acaccaattt atcaggcaga tactctggtc caggttgagc
     2101 aaaaacaggg caacgccatt ctcagcggcc tgagcgatat gatccctaac tcatcgcccg
     2161 agtctgcacc ggagatccaa ctgctgcaat cgcgcatgat tctcggtaaa accattgctg
     2221 aactgaatct gcgtgacatg gttgagcaaa agtattttcc gattgtgggt cgcggctggg
     2281 cgagattaac caaagagaaa ccaggtgagc tggcgatcag ctggatgcat attccacaac
     2341 tgaatggtca ggatcagcaa ctgacactca cggttgggga aaacggccac tatacgctgg
     2401 aaggtgaaga gttcaccgtc aatggtatgg tcggccagcg tctggaaaaa gatggcgttg
     2461 cgctgactat cgcggacatt aaggccaaac caggaacaca gtttgtcctg agccaacgta
     2521 ccgaactgga agcgattaac gcgttgcagg aaacctttac cgttagcgaa cgcagtaaag
     2581 aaagcgggat gctggaactt accatgaccg gtgatgatcc ccagttgatt actcgtattc
     2641 tgaacagcat cgctaacaac tatttgcaac agaatatcgc tcgccaggcg gcgcaggatt
     2701 cacaaagcct tgaattctta cagcgccagt tgcctgaagt gcgcagcgag ctggaccagg
     2761 cggaagaaaa actcaacgtt tatcgccagc agcgcgattc ggttgacctt aacctggaag
     2821 ccaaagccgt tcttgagcag attgtgaacg ttgataatca actcaatgag ctgactttcc
     2881 gcgaggcaga gatctcccag ctgtataaga aagatcaccc aacttatcgt gcgctgctgg
     2941 aaaaacgcca gacgctggag caagaacgca aacgcctgaa taagcgggta tcggctatgc
     3001 cttccaccca acaggaagtg ttgcgtttaa gtcgtgacgt agaagcgggc cgtgcggtat
     3061 atctgcaatt acttaaccgc cagcaggagt tgagtatttc gaaatccagt gccattggta
     3121 acgtgcggat tatcgacccg gcagtcactc agccgcagcc agtgaaaccg aaaaaagcgt
     3181 tgaatgtggt gcttggtttt attcttggcc tgtttatttc ggtgggtgcc gtgctggcgc
     3241 gtgcgatgtt gcgtcgtggt gtagaagccc cggaacaact ggaagagcac ggcatcagcg
     3301 tttatgccac tatcccaatg tccgagtggc tggataaacg tacccgtctg cgtaagaaaa
     3361 atttattttc taatcagcag cgccatcgta ctaaaaatat ccccttcctg gctgtggata
     3421 acccggcgga ttctgctgtg gaagccgtac gtgcgctacg aaccagtctg cacttcgcta
     3481 tgatggagac ggagaataac attctgatga tcaccggtgc gacgccagac agtggtaaaa
     3541 cgtttgtcag ttcaactctg gcagcggtga tcgcccagtc cgatcaaaaa gtgttattta
     3601 ttgatgccga cttacgccgc ggttattcgc ataacctgtt tacagtgagt aatgaacatg
     3661 gcttgtcgga atatctggca ggtaaagatg agctcaacaa agtgatccag cattttggca
     3721 aaggaggctt tgatgtgatt actcgcggtc aggtgccacc taacccgtct gaactgctga
     3781 tgcgcgatcg gatgcgtcaa tta
//
LOCUS       DAHBHC010000199         3760 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHBHC010000199 DAHBHC010000000
VERSION     DAHBHC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.203"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..357)
                     /locus_tag="M2A35_005140"
     CDS             complement(<1..357)
                     /locus_tag="M2A35_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCB7201297.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNY"
     gene            complement(376..837)
                     /gene="tsaE"
                     /locus_tag="M2A35_005141"
     CDS             complement(376..837)
                     /gene="tsaE"
                     /locus_tag="M2A35_005141"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCB7201298.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(809..2356)
                     /gene="nnr"
                     /locus_tag="M2A35_005142"
     CDS             complement(809..2356)
                     /gene="nnr"
                     /locus_tag="M2A35_005142"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCB7201299.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            2355..3494
                     /gene="queG"
                     /locus_tag="M2A35_005143"
     CDS             2355..3494
                     /gene="queG"
                     /locus_tag="M2A35_005143"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCB7201300.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLTLNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(3477..3530)
                     /locus_tag="M2A35_005144"
     CDS             complement(3477..3530)
                     /locus_tag="M2A35_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201301.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          906 a         1052 c          949 g          853 t
ORIGIN      
        1 gtaattgctg ccattctgcc gcttcaccgc ttcggtttta ccgttttcgg taagatcgac
       61 caccagccgt agcgtttgtg catctttagg cgttccagag cgaatcgcct tcaccagatt
      121 attgccgctg aacaacaacg gcagtccctg aatcacgccc gtttgtttga tatcgagcgc
      181 cacggtgcgt ttgctttgat ggctaaacgc ataatcagga tcgccaataa aactcaacgt
      241 tatccgcgcc tgttgattac cgttagaaac ctgaatatca gagagcgtcg cggcacccac
      301 cggcgtgcac agcagcagca gcgtcgctac caaccaattt ctgatgcgat acatcatccc
      361 gccacctttc aaaggttaac cggctaaacg cgccagcaac aattcacccg cagaggaaac
      421 cgcactcacg cgcgcctcac ggccttgtgc ctgataatca atgtgtattt cgacatccgg
      481 gtcaggaaga acaccagtac cttgttgtgg ccactccacc aggcagatgg catcgttggc
      541 aaaataatcg cgaatcccca taaactccag ctcctcggga tcggcaaggc ggtacaaatc
      601 aaagtgataa accattaaat tgtcgagcgt atagggttcg accagcgtat aagtggggct
      661 tttgacatta ccctgatgac ccagagcttg taaaaagccc cggctaaagg tggttttacc
      721 tgcgcctaaa tcgccataca gatagattac ggttgcgcca tcgcaggctt tcgctacccg
      781 ctcgcccagg tctaatgttg cctgctcatc agggagcgga attactcgat tcatcatggt
      841 ttttatcagt cacttccggg ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca
      901 gcatcccgcg cgttccaaaa cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac
      961 agcccgcaca ggcggcatca tacggcgaca gtttttgccc aagcaatgcg ccaataatac
     1021 cagagagcac atcgcccatg ccgccgctcg ccatgcctgc atttccggca tcaataatgc
     1081 ctaaagcgtc aggatgggcg gcgaccacgg ttccggcacc tttcagcacc gctacgccgc
     1141 cataacgttg taccagacgt ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg
     1201 aacagcctaa caaccgtgcg gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct
     1261 tatcgggatt gattgccagc aggttcaatg catcggcatc ccacaacatc ggtttgcgaa
     1321 aattctcaac tttttgcagt gcttttttcc cccactcttg ctggcccaga ccgggaccaa
     1381 tcaccaccac atcggcccat tccaggcttt cggtaagaga gtccatcgtc agttcatgca
     1441 ccatcaattc cggtcgtgca gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc
     1501 ggactaaacc agcaccagca cgtagcgccg cttcccccgt catacgaata gcccccgccg
     1561 tgccgtgatc gccaccgata attaccagcc gcccgtgatc gcctttatgc gaagtcgggc
     1621 gacgcggttt tagccagtga gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct
     1681 gacctgccag ccaactatcc agccccagtg agtcaaaatg cagttgtccg gtaacatccc
     1741 gcgcttttcc agtgagcaag cctggtttca gcgcaataaa agtgatggtg tgatcggcgt
     1801 tgatcactgc gcctggcgta gcgccggttt cagccagcag gccggaaggg atatcaaccg
     1861 ccgcaatcgg cgcaggatgg gaattagcgt ggtcgattaa ctggctaatg gattcgcggg
     1921 gcgcttgctg caagccggta ccgagcagcg catcaacaat cagatctacc gattcgggcc
     1981 agacaatatt cgaagcatgg atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg
     2041 cggcctcttc cggcaacggt ttgtcgctct cctgggccag cagcgtgacc tcaatgccga
     2101 ccgctgtggc cagccgcgcg accacgtagc catcgccgcc gttattacca tgaccgcaca
     2161 gcaccagcca atggcgggcg tcaggatacg ccgaacgaca cacctggaat gcggcttcgc
     2221 cagcgcgaag catcagctca tagagtgtaa gccccagcgc atctgccgcc tcacgttctc
     2281 cgcggcggat atcgtcggcg tgccagacgg tgtgtggtat acttacgggg tttttcttca
     2341 ttgtatggtc cgtcatgtca gagcccctcg atctcaatca gttagcgcaa aaaattaaac
     2401 agtgggggct ggaactgggc tttcagcagg taggtattac cgataccgat ctcagcgagt
     2461 ccgagcccaa actgcaagca tggctggaca aacaatacca cggcgaaatg gactggatgg
     2521 cacgtcacgg tatgctgcgc gctcgccccc atgagttatt gcccggtacg ctgcgcgtga
     2581 tcagcgtgcg gatgaattac cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc
     2641 ccaaactcgg ctatgttagc cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca
     2701 accgactcaa aaagctgggc gagatgattc agcaacattg tgtttcgctg aattttagac
     2761 cgtttgtcga ttctgcgcct attctcgagc gcccgttagc tgaaaaagct gggctcggct
     2821 ggacaggtaa gcactcactt accctcaatc gcgaggccgg ttctttcttc tttttaggcg
     2881 aattgctggt cgatattccg ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat
     2941 gcgtggcctg tatgacgatt tgcccgaccg gtgccatcgt cgagccatat accgtcgatg
     3001 ctcgccgctg tatctcttat ctcaccatcg aacttgaagg ggcgatcccg gaagagttgc
     3061 gaccgttaat gggaaaccgt atttacggtt gcgatgactg ccagcttatc tgcccgtgga
     3121 atcgctattc acaactcacc acagaagagg atttcagccc gcgtaagccg ctacacgcac
     3181 cggaactcat tgagttattc gcctggagcg aagagaagtt tttaaaagtc acggaaggat
     3241 cggcgattcg tcgtattggt cacctgcgtt ggctgcgtaa tatcgccgta gcattaggca
     3301 atgccccttg ggatgaaacg attttgacag cgctggaaag tcgtaaaggt gagcacccac
     3361 ttctcgatga gcacatagcg tgggcgattg cgcagcaaat agagagacga aatgcgtgca
     3421 tagtcgaagt gcaattgccg aaaaaacagc gtctggttcg ggtgattgaa aaagggttac
     3481 cgcgtgacgc ctgattcatc cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa
     3541 gacgaataaa atcgtcaagt gatcgactcg acaaaacgga atcttaattt ttaacttatc
     3601 tatcaatagg ttaaaaaaat actattcacc gtgcgaagtt tctttgaaat taagattaca
     3661 gcctgaggct gtggataact ctgtttacaa gagtttgtca gagacaacga aaaccagcaa
     3721 taacgcatcg catcgctgtg gatattttat tgagagaaga
//
LOCUS       DAHBHC010000200         3759 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHBHC010000200 DAHBHC010000000
VERSION     DAHBHC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.204"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(29..1171)
                     /gene="caiA"
                     /locus_tag="M2A35_005145"
     CDS             complement(29..1171)
                     /gene="caiA"
                     /locus_tag="M2A35_005145"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCB7201302.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(1202..2716)
                     /gene="caiT"
                     /locus_tag="M2A35_005146"
     CDS             complement(1202..2716)
                     /gene="caiT"
                     /locus_tag="M2A35_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCB7201303.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            3190..>3759
                     /gene="fixA"
                     /locus_tag="M2A35_005147"
     CDS             3190..>3759
                     /gene="fixA"
                     /locus_tag="M2A35_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative electron transfer flavoprotein FixA"
                     /protein_id="HCB7201304.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIP"
BASE COUNT          970 a          964 c          910 g          915 t
ORIGIN      
        1 tcagcgtcgc atcaggcagc gcattgactt agcggtattg cttcagcacc gcacgaccca
       61 gcgtcaggat ctgcatttcg tcagatcccc cggagacgcg gtctacacgc aggtcacgcc
      121 agaagcggct gatgcggtgg ttgcccgcaa tcccgacacc gcccagcacc tgcattgcgc
      181 tatccaccac ttcaaatgcc gcattggcgc agaagtattt gcacatcgct gcatcgccag
      241 aggtgatggt gccgttgtct gctttccacg ctgcttcata cagcatgttt ttcatggagt
      301 ttaatttgat cgccatgtgg gcgaattttt cctgaatcaa ctggaaacga ccaatagcct
      361 cgccaaactg cacgcgctgg ttggcgtagc gcgccgcatc ttcaaaggcg cacatcgccg
      421 taccgtagtt ggtcagggct accaggaaac gttcatggtc gaactcttct ttgacgcggt
      481 taaagccgtt accttcccga ccgaacatgt ctttctcgtc cagttccacg tcgtcaaagg
      541 tgatttcaca gcagctatcc atacgcagac cgagcttttc aagtttggtc actttgatgc
      601 ccggtttgct catatcaaca aaccattcgg tgtagacagg tttgtccgga gaagccccgt
      661 cgcgcgccat caccacgatg tacggggtgt aggcgctgct ggtaataaaa cacttactac
      721 cattaagata aatcttacca tttctacggg tataagtcgt tttcaggcta cccacgtcgg
      781 agcccgcgcc cggttcggta atcgctgagt tccacatctg cttaccggtg ccgcggaaag
      841 ccataatctt gtcgatctgc tcttgtgtgc cttcgcgcag gaaggtgttg aacccgcccg
      901 gcaactggta cagtacatag gttggtgccc ccagacgtcc cagctccatc cacacggcgg
      961 cgagagtaac aaaccccgcg tccagaccac cgtgctcttc agggatcagc agactgtcga
     1021 tacccatatc cgccagtgct ttgacaaaac gttccgggta gacgctgtca cggtcgcact
     1081 cggcaaaata ggcctcccag ttttcgctgg ccatcagttc gcggataccg gcgacaaaca
     1141 gttcctgctc atcatttaaa ttaaaatcca tctttcaacc tcttgatatt ttgggggtta
     1201 attaatcttt ccagttctgt ttcgcgtctt taataaagga gagcgtcacc ataatgttga
     1261 cgaagaacag cgggcatcct ccggcgataa tggcagtttg aatcggtttc aggccgccga
     1321 gcgccagcaa aacaataccg ataatgccaa ccagaattga ccaaccgata cgcaccagca
     1381 gcggtggttc ttcaccatcg cgtacttcgc ggcaagtgga catcgccagg gtataagagc
     1441 aggcgttaac cagcgtaacg gtggcaataa agcagaggat gaagaagccc cacatggtgg
     1501 cggtgctgag tggcagagcg gcccaggttt cgataatggc gcgcgccaca ccgtactgtt
     1561 cgatcagatt tggaatgttg ataatgtttt tatctatcaa cagcagagtg ttactaccga
     1621 gtacagtcca caggatccag gttgacgctg tcagccccag caccatgccg aagcacagtt
     1681 cacgcacagt acgaccacgg gagatgcggg cgaggaagat actcatctgg atagcataaa
     1741 tcacccacca tgcccagtag aacacggtcc agccctgcgg gaagccgcct ttagcgatgg
     1801 gatcggtata gaacaacatg cgcggcagat acatcagcaa catccccacc gaatcggtga
     1861 agtagttcat gatgaagctg gcaccgctga caatgaacac ccaacccagc atcaggaagc
     1921 tcaggtaact acgcacgtca ctggcgatac gtaccccttt ttgcagaccg caagcgacgc
     1981 aaatggcgtt gaggataatc cagcaggtaa tgatgatagc gtccagttgc agggtatgcg
     2041 gaatgccaaa caaccattgc atacactcgg tcaccagcgg cgtggcaagg cccagactgg
     2101 tacccatcgc gaagatcaag gcgacgagat agaagttgtc gacgatagtg ccgaacaacc
     2161 ctttggcgtg tttttcacct accagcggca ccagtgtcga gctggggcga atcacttcca
     2221 ttttgcggac aaagaagaag taagcgaagg cgactgaaag gaagctgtaa gtggcccacg
     2281 gcagaggtcc ccagtggaac aagctgtaag ccagccccaa ctctttcgcc cctgtcgagt
     2341 tcggttctaa gccaaacggc ggggtggaga tgtagtagta gatctcaatc gatccccaga
     2401 acagtacggc agcagacgta caggaggcga acatcataaa gatccaactg gcggtactaa
     2461 attcaggtgg ttcgttacct aaacgctttt tggcatacgg gccaaacacc agccagaacc
     2521 aaccgaaaag catcaccacc atataccatt caaatgccca tccccataca ttggtgacgt
     2581 aactgaatac agcattaata acgacattcg ctgcatccag atctctgact gtaagccaac
     2641 aaagtatgcc gacgattatt aacggcggaa agaaaacctt cggttctatt cccgtttttc
     2701 tcttttcatt cttcatgagt taattccact gtgaaaacga atatttattt tgcgttcccg
     2761 tttgttttat ttttgtcaac atttaatata attattatta acctcgtgga cgcgttaatg
     2821 gctaactcat aatgggtatt caataagctg cattctgtga ttgttatcac atttttgttt
     2881 cgggtgaata gagggcgctt ttctcgttaa ttttgattat taatcagttt gttatgttgt
     2941 gttgtgagta aaaaaatagc atctgacttt caatattggt gatccataaa gcaatattga
     3001 aaatttcttt ttgctacgcc atgttttcaa tattggtgag gaacttaaca atattgaaag
     3061 ttggatttat ctgcgtgtga cattttcaat attggtgatt aaagttttat ttccaaatta
     3121 aagggcgtga tatctgtaat taacaccacc gatatgaacg acgtttcctt catgatttct
     3181 ggagatgcaa tgaagattat tacttgctat aagtgcgtgc ctgatgaaca ggatattgcg
     3241 gtcaataatg ctgatggttc attagacttc agcaaagccg atgccaaaat aagccaatac
     3301 gatctgaacg ctattgaagc ggcttgccag ctaaagcaac aggcagcaga ggcgcaggtg
     3361 acggccttaa gtgtgggcgg taaagccctg accaacgcca aagggcgtaa agatgtgcta
     3421 tcgcgcggcc cggatgaact gattgtggtg attgatgacc agttcgagca ggcactgccg
     3481 caacaaacgg cgagcgcact ggctgcagcc gcccagaaag caggctttga tttgatcctc
     3541 tgtggcgatg gttcttccga cctttatgcc cagcaggttg gtctgctggt gggcgaaatc
     3601 ctcaatattc cggcagttaa cggcgtcagc aaaattatct ccctgactgc agataccctc
     3661 accgttgagc gcgaactgga agatgaaacc gaaaccttaa gcattccgct gcctgcggtt
     3721 gttgctgttt ccactgatat caactcccca caaattcct
//
LOCUS       DAHBHC010000201         3580 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHBHC010000201 DAHBHC010000000
VERSION     DAHBHC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.206"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..420)
                     /locus_tag="M2A35_005148"
     CDS             complement(<1..420)
                     /locus_tag="M2A35_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCB7201305.1"
                     /translation="MEFKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAESW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVI"
     gene            complement(562..2469)
                     /locus_tag="M2A35_005149"
                     /pseudo
     CDS             complement(562..2469)
                     /locus_tag="M2A35_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2489..2950)
                     /locus_tag="M2A35_005150"
     CDS             complement(2489..2950)
                     /locus_tag="M2A35_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCB7201306.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(2978..>3580)
                     /locus_tag="M2A35_005151"
     CDS             complement(2978..>3580)
                     /locus_tag="M2A35_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201307.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRSTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT          737 a         1003 c          912 g          928 t
ORIGIN      
        1 gatgaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg
       61 actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt ctttatcatt
      121 tgatgaatgg cagtcacgca tccagttaat aaagcactca tgaaattttg caggactgat
      181 acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa aatcaccata
      241 ttgcttcaag aaatcgagat gtgtttcccc gaaatcctct atatcttccc aactttctgc
      301 accagaaata acggcacaaa tagtcaacaa tagaatatcc gataatttat gttccacttt
      361 ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcaattttt taaattccat
      421 tttgttctcc ttaattatgt aaggagtatt tgatcatgta taagcaataa aaaacagctt
      481 caggtaataa ggaatatctc aatttttaaa cataaaatgc gaattattta gtacaaaaaa
      541 ccggcgttac atgatctttc cctggctcca cgtctggtcg ttatcggacc agcggatgat
      601 ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat ccgcttcggt
      661 cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg
      721 gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt
      781 gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc ggttgctggc
      841 ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg ccaggcggtc
      901 atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg cgtcataaaa
      961 gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc ggcggcgcag
     1021 aatgtactgg gcgtgatacg ggttaaccgt cgtatcactg tcaccgtcga aggcaaatct
     1081 cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca cccaggcgta
     1141 ttcgctccag gagtcgctcc agcccacgcc cgtcaggcca tgcagcggca gtacagaact
     1201 gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg tacgttcgtc
     1261 gcagtacgcg ccggttaccg gatcgacagg atctgttacc catctgatga ctttattggc
     1321 aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg cgcccagcag
     1381 cttcccgctt ttccccagca ggtttcccag tttgcccagt ttcgccagat tccccagttt
     1441 tcccagcagt cctgctggcg gcagaagata gctggctatc gttattcctc ccaggatcat
     1501 tctctgccat ggcgggaact ccggctggat atccagatag gtgacctgct cacccccaat
     1561 aaagacgtcc ggcgaacctt ccacgatcgc cgcactgcac tccagtttgt cgcccttgcg
     1621 cgcggcaggc tgattgttga taaagacgtt gctactgccc tgggctatcc tcggtggttt
     1681 gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca cctctttttt
     1741 gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac cggcgggaaa
     1801 cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgcttt ccgtcagggt
     1861 gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg ccgattcaag
     1921 caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggatttcag gtattgctcc
     1981 aggtggggga ggagggggaa gggtgacggc ggcccttgca gcgggctttc cgttaatgtt
     2041 tacattttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt ttatccatga
     2101 ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg catacatcag
     2161 cgcaggcacc agaaccagcg cataagggcc ggtggcgcag gcggccataa tcaggcaacc
     2221 ttctgcgatg gtcactatcg cccccagcac ggcccctgcc agtgccccca gaaaactttt
     2281 atgctgaatt tcatcgccca gcctggcggc aggtgggccg gacttctttg ctgccagcat
     2341 ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca tcgcctgctg
     2401 tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc cgcctggtcc
     2461 ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg atgtcattct
     2521 ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt tgactgttct
     2581 tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc cgctggaata
     2641 cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc caggaataat
     2701 ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt tcggtgtacc
     2761 catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt gtcagggttc
     2821 cctgtggaac ggcttcgcgg gtaatgacca ggttgatgcc actgttatct gtggacacca
     2881 ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct tcattaaaag
     2941 tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa acagaaccgc
     3001 ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg acctgtagct
     3061 gaatgccatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa atttccttaa
     3121 tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca
     3181 ccgaaccggc aatcgtgctt ctgtcctgaa agacatcctc agataaagac cctttcagta
     3241 ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc tggatcgtga
     3301 ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt
     3361 tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct ctccctgtcg
     3421 catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag cggatggtca
     3481 tctgcgtctt tgttcccggc aggctgccgg gggtgcggtt ttcctggtgg taggtgcgcc
     3541 ccataatgat cggctggtcc ggatcgccgt tgaggaagtc
//
LOCUS       DAHBHC010000202         3580 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHBHC010000202 DAHBHC010000000
VERSION     DAHBHC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.207"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..636
                     /gene="tolA"
                     /locus_tag="M2A35_005152"
     CDS             <1..636
                     /gene="tolA"
                     /locus_tag="M2A35_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCB7201308.1"
                     /translation="AATEAAEKAKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAA
                     AEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGG
                     GAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLA
                     PDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            769..2061
                     /gene="tolB"
                     /locus_tag="M2A35_005153"
     CDS             769..2061
                     /gene="tolB"
                     /locus_tag="M2A35_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCB7201309.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPVAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            2096..2617
                     /gene="pal"
                     /locus_tag="M2A35_005154"
     CDS             2096..2617
                     /gene="pal"
                     /locus_tag="M2A35_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCB7201310.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            2627..3418
                     /gene="cpoB"
                     /locus_tag="M2A35_005155"
     CDS             2627..3418
                     /gene="cpoB"
                     /locus_tag="M2A35_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCB7201311.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
BASE COUNT          960 a          856 c          957 g          807 t
ORIGIN      
        1 gcggcaactg aagctgctga aaaagccaaa gcagaagctg agaagaaagc ggctgctgaa
       61 aaggctgcag ctgataagaa agcggcagca gagaaagctg cagccgacaa aaaagcagca
      121 gaaaaagcgg ctgctgaaaa ggcagcagct gataagaaag cagcggcaga aaaagccgcc
      181 gcagacaaaa aagcggcagc ggcaaaagct gcagctgaaa aagccgctgc agcaaaagcg
      241 gccgcagagg cagatgatat tttcggtgag ctaagctctg gtaagaatgc accgaaaacg
      301 gggggagggg cgaaagggaa caatgcttcg cctgccggga gtggtaatac taaaaacaat
      361 ggcgcatcag gggccgatat caataactat gccgggcaga ttaaatctgc tatcgaaagt
      421 aagttctatg acgcatcgtc ctatgcaggc aaaacctgta cgctgcgcat aaaactggca
      481 cccgatggta tgttactgga tatcaaacct gaaggtggcg atcccgcact ttgtcaggct
      541 gcgttggcag cagctaaact tgcgaagatc ccgaaaccac caagccaggc agtatatgaa
      601 gtgttcaaaa acgcgccatt ggacttcaaa ccgtaatcgc gatgttgact gttcggacgg
      661 tcaacatcag gcaccggttg ccacggggtt ctggtagttt tgtgtatttt agtttgttaa
      721 cattctgcta aattatcgtg ggccatcggt ccagataagg gagatatgat gaagcaggca
      781 ttacgagtag catttggttt tctcatactg tgggcatcag ttctgcatgc tgaagtccgc
      841 attgtgatcg acagcggtgt agattccggt cgtcctattg gtgttgttcc tttccagtgg
      901 gcggggcctg gtgcggcacc tgaagatatt ggcggcatcg ttgctgctga cttgcgtaac
      961 agcggtaaat ttaatccgtt agatcgcgct cgtctgccac agcagccggg tagtgcgcag
     1021 gaagtacaac cagttgcatg gtccgcactg ggcattgacg ctgtagttgt cggtcaggtc
     1081 actccgaatc cggatggttc ttacaatgtt gcttatcaac ttgttgacac tggcggcgca
     1141 ccgggtactg tacttgctca gaactcgtac aaagtgaaca agcagtggct gcgttatgct
     1201 ggtcataccg ccagtgatga agtatttgaa aaactgaccg gcattaaagg tgcgttccgt
     1261 actcgtattg cctacgttgt tcagaccaac ggcggtcagt tcccgtatga actgcgcgta
     1321 tctgactatg acggttacaa ccagtttgtc gttcaccgtt caccgcagcc gctgatgtct
     1381 ccggcgtggt caccagacgg ttctaaactg gcttatgtga ccttcgaaag cggtcgttcc
     1441 gcgctggtta ttcagacgct ggcaaatggc gctgtacgtc aggtggcttc attcccgcgt
     1501 cacaacggtg cacctgcatt ctcgccagac ggcagcaaac tggcattcgc cttgtcgaaa
     1561 accggtagtc tgaacctgta cgtaatggat ttggcttctg gtcagatccg ccaggtgact
     1621 gatggtcgca gtaacaatac cgaaccgacc tggttcccgg atagccagaa cctggcattt
     1681 acttctgacc aggccggtcg tccgcaggtt tataaagtga atatcaacgg cggtgcgcca
     1741 caacgtatta cctgggaagg ttcgcagaac caggatgcgg atgtcagcag cgacggtaaa
     1801 tttatggtaa tggtcagctc caatggtggg cagcagcaca ttgccaaaca agatctggca
     1861 acgggaggcg tacaagttct gtcgtccacg ttcctggatg aaacgccaag tctggcacct
     1921 aacggcacta tggtaatcta cagctcttct caggggatgg gatccgtgct gaatttggtt
     1981 tctacagatg ggcgtttcaa agcgcgtctt ccggcaactg atggacaggt caaattccct
     2041 gcctggtcgc cgtatctgtg ataataatta attgaatagt aaaggaatca ttgaaatgca
     2101 actgaacaaa gtgctgaaag ggctgatgat tgctctgcct gttatggcaa ttgcggcatg
     2161 ttcttccaac aagaacgcca gcaatgacgg cagcgaaggc atgctgggtg ccggcactgg
     2221 tatggatgcg aacggcggca acggcaacat gtcttccgaa gagcaggctc gtctgcaaat
     2281 gcaacagctg cagcagaaca acatcgttta cttcgatctg gacaagtacg atatccgttc
     2341 tgacttcgct caaatgctgg atgcacatgc aaacttcctg cgtagcaacc cgtcttacaa
     2401 agtcaccgta gaaggtcacg cggacgaacg tggtactccg gaatacaaca tctccctggg
     2461 tgaacgtcgt gcgaacgccg ttaagatgta cctgcagggt aaaggcgttt ctgcagacca
     2521 gatctccatc gtttcttacg gtaaagaaaa acctgcagta ctgggtcatg acgaagcggc
     2581 atactccaaa aaccgtcgtg cggtactggt ttactaagag aattgcatga gcagtaactt
     2641 cagacatcaa ctattgagtc tgtcgttact ggttggtata gcggccccct gggccgcttt
     2701 tgctcaggca ccaatcagta gtgtcggctc aggctcggtc gaagaccgcg tcactcaact
     2761 tgagcgtatt tctaatgctc acagccagct tttaacccaa ctccagcaac aactttctga
     2821 taatcaatcc gatattgatt ccctgcgtgg tcagattcag gaaaatcagt atcaactgaa
     2881 tcaggtcgtg gagcggcaaa agcagatcct gttgcagatc gacagcctca gcagcggtgg
     2941 tgcagcggcg caatcaacca gcagcgatca aagcggtgcg gcggcatcaa cgacgccgac
     3001 agctgatgct ggtactgcga atgctggcgc gccggtgaaa agcggcgatg caaacacgga
     3061 ttacaatgca gctattgcgc tggtgcagga taaatcccgc caggatgacg caatggtggc
     3121 atttcagaat ttcatcaaaa attaccctga ttcaacttac ctgccaaacg ccaattattg
     3181 gctgggtcag ttaaactaca acaagggtaa aaaagatgat gcggcgtact attttgcttc
     3241 ggtagtgaaa aactatccga agtcaccaaa ggctgctgat gcgatgttta aagtcggcgt
     3301 catcatgcag gacaaaggtg acaccgcaaa agcgaaagcc gtataccagc aggttatcag
     3361 taaataccct ggtactgatg gcgctaaaca ggcacaaaaa cgtctgaacg cgatgtaatg
     3421 cataacacac gaccagaagt cgcattattt ctggtcgtgt cgtgcgaatc ataagcagtt
     3481 gagtgatctg catcgaaatt tttgttgcgc tcaagtctga aatcagtaat atatgccgcc
     3541 gttgccacgg gatatcaaac aaaccgaaag caacgaaaaa
//
LOCUS       DAHBHC010000203         3569 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHBHC010000203 DAHBHC010000000
VERSION     DAHBHC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.208"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..286
                     /locus_tag="M2A35_005156"
     CDS             <1..286
                     /locus_tag="M2A35_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7201312.1"
                     /translation="RPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGT
                     KSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            299..673
                     /locus_tag="M2A35_005157"
     CDS             299..673
                     /locus_tag="M2A35_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7201313.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKSGERYRRDVALIVRQK
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGL
                     PVPGGRLGVKIYEIMHDGQVKK"
     gene            670..1491
                     /locus_tag="M2A35_005158"
     CDS             670..1491
                     /locus_tag="M2A35_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7201314.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRVRATELLADYALSQCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCRGEKFIESEVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKIREVVKVSCPECKGKGEISTACKDCRGRGVAIHREESEKRGMPVIRDCRRCGGRGY
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            1719..1916
                     /locus_tag="M2A35_005159"
     CDS             1719..1916
                     /locus_tag="M2A35_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCB7201315.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQP"
     gene            2067..3125
                     /locus_tag="M2A35_005160"
     CDS             2067..3125
                     /locus_tag="M2A35_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCB7201316.1"
                     /translation="MTNTVKISSCELINADCLKFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDADYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFSATERILFAEHYQGPYQPKNDGYAAKGRELKQHVMA
                     PLISYFRDARESLGITSKQIAEATGKKNMVSHWFGISQWQLPNEGDYLKLQALFARVA
                     AEKHQRCELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVQVPYTDVWTHKPM
                     HYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            3166..3241
                     /locus_tag="M2A35_005161"
     tRNA            3166..3241
                     /locus_tag="M2A35_005161"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3199..3201,aa:Ile,seq:cat)
     gene            3243..3319
                     /locus_tag="M2A35_005162"
     tRNA            3243..3319
                     /locus_tag="M2A35_005162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3277..3279,aa:Arg,seq:tcg)
     gene            3333..3409
                     /locus_tag="M2A35_005163"
     tRNA            3333..3409
                     /locus_tag="M2A35_005163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3367..3369,aa:Arg,seq:tct)
BASE COUNT          924 a          805 c          986 g          854 t
ORIGIN      
        1 acgacctaaa cgtcgccgct gggttaacga gaaatacacg cgctgggtaa agacacagcc
       61 gtgtgcgtgt tgtggtaagc cagccgacga tccccatcac ctgattggtc atggtcaggg
      121 cggaatggga acaaaatccc acgatatttt cacgctaccg ttgtgccggg aacatcacaa
      181 tgaacttcat gcggatccgc tggcgttcga agaaaagcat ggttcccagg ttgatttaat
      241 ttttcgtttt cttgatcacg cctttgcaac cggcgtgctc gggtaaaaga ggttactgat
      301 gcgtatagag tttgttttgc cttacccgcc gacggtgaac acctactggc gacgtcgtgg
      361 cagcacatat tttgtatcaa aatccggtga gcgttatcgc cgtgatgtgg cgctcattgt
      421 tcgccagaag cggttgaaat taaacctgtc cggaaggctg gcgataaaga ttattgcaga
      481 gccaccggat aagcgccgtc gtgacctgga caatatcctg aaagcaccac tggatgcgct
      541 gacgcatgcc ggactactca tagacgacga gcagtttgat gaaattaata ttgtgcgcgg
      601 tctgcctgtt cctggtggtc ggttgggcgt gaaaatttac gaaataatgc atgacgggca
      661 ggtcaaaaaa tgaaactgga agatttaccg aaatactatt ccccgaaatc gccaggcctg
      721 actgatgcat ccgcctcgac atcaaaagat gcactgagta tcactgatgt gatggctgca
      781 cagggcatga cacagaaccg ggctgagatg ggattttctg cgttcctggg gaaaatgggc
      841 attagtatga acgacagggt gcgggcaaca gaattactgg cagattatgc attaagtcag
      901 tgcgatcgcg tggcggcgtt aagaaaactt ccggcagaaa taaaaccggc agtgatgcgc
      961 attatggctt cgtatgcttt tgaggattat gcccgcagtg cagcgagtaa aaagcagtgt
     1021 tcttgttgcc gtggggaaaa atttattgaa agcgaagttt ttacaaacaa ggttcagtat
     1081 ccggatggta agccgccagt atgggcaaag tgcacaaaag gcgtgtatcc gtcttattgg
     1141 gaagaatgga aaaaaatccg ggaggtggtg aaagtttcct gtccagaatg taaagggaag
     1201 ggagagatct ccactgcctg taaagactgc cgtgggcgtg gtgttgccat tcatcgtgaa
     1261 gagtcagaaa aacggggtat gcctgttatc agggactgcc ggcgttgtgg cggtcgtggc
     1321 tatgaaagac tgccatcaac ggaggcattt aatgccatat gcaaagtgac gagtgctatc
     1381 acgcttgata catggaaaaa atcagtgaaa cgcttttacg atacgttggt ggttcggttt
     1441 gacattgaag aggcatgggc ggagcggcag ttaaagaggg taacgcgata gtgttgttga
     1501 tttttcccga atctgtggta aattcaccct gatgatgggc gttttatgcc tgacgttaga
     1561 agaattttta caacccgcca ccgagcgggt tttttattgc ctgaaaaacg gcacaggacg
     1621 ttaaacgcgc tggtggttgc gaataccggt ctttcaactt gctggctttt tcgacaagag
     1681 ttattggtat gtcacgttaa ccaaaaggga aaaaagacat gctaaaacag caggatatga
     1741 cagaaaccgc cagagtggtg tttaatgaat taagcgttac cgacccggcg acagtcgggg
     1801 agattgcgca gaatacttac ctttcacgcg aacgctgcca gttaatactg acccagcttg
     1861 ttatggcggg tctggcagat tatcagttcg gttgttacag acgccttcag ccctgaaggc
     1921 ttttttattt gtggtaatgg gcggctggtg ggtgttagcg gcacctgcca gccatctgct
     1981 catgcgttgg ggtcacaagc aaacctcagg cccatctgct ttgcgcaaaa gcagaatgag
     2041 cctatcagag aagtgcttat tgatctatga ctaacactgt aaaaatatcc agctgtgagt
     2101 taatcaacgc tgattgcctg aaatttatcc agaccttacc ggaaaactct gtcgatctga
     2161 tagtcacaga cccgccatac tttaaagtga aacccgaagg ctgggataac cagtggaagg
     2221 gggacgctga ttacctgaaa tggctggacc agtgccttgc gcagttctgg cgggtattaa
     2281 aacctgccgg aagtctttac ctgttctgtg gtcatcgtct ggcatctgat accgaaatca
     2341 tgatgcgtga acgctttaat gtgctgaacc acattatctg ggcgaagccg tccggacgct
     2401 ggaacggatg caacaaggaa agcctgcggg cgtatttctc ggcaacagaa cgcattctgt
     2461 ttgcagaaca ttatcagggg ccataccagc ccaaaaatga cggctatgcg gcaaaggggc
     2521 gcgagctaaa acagcacgtc atggccccgc tgatttctta ctttcgtgat gcgcgtgaat
     2581 cactgggaat aacgtcaaaa cagatagcgg aagccaccgg aaagaaaaac atggtttcgc
     2641 actggtttgg tatcagtcag tggcagttgc cgaacgaagg cgattatctg aaattacagg
     2701 cgttgtttgc gcgtgttgca gcagaaaaac atcaacgctg tgaactggaa aagccacacc
     2761 accagctggt cagcacatac agtgaactga accggcagta tacggaactg cagagtgaat
     2821 ataagcatct gcggcggtat ttcggtgtga cggtgcaggt gccgtacacc gatgtgtgga
     2881 cgcataaacc gatgcattac tatcccggga aacatccgtg cgaaaaaccg gcagaaatgt
     2941 tgcagcagat aatcagcgca agcagtcgtc cgggagacct ggttgcagat ttcttcatgg
     3001 ggtcgggttc gacagtcaaa gcagcgatgg cgctgggacg tcgtgcaact ggtgttgaac
     3061 tggagactga acgttttgag cagacggttc gggaagtaca ggatttagtc agccagaacg
     3121 gatgatattg aagaattaat tatgcaccgt tattattctg ctcccggccc tttagctcag
     3181 tggtgagagc gagcgactca taatcgccag gtcgctggtt caaatccagc aagggccacc
     3241 aaccgccatt agctcatcgg gatagaacgc cagccttcga agctggtttc gcggggttcg
     3301 agtctccgat ggcggtccat tatcggtatt ctgcgttgtt agctcagccg gacagagcaa
     3361 ttgccttcta agcaatcggt cactggttcg aacccagtac aacgcaccac acttattttc
     3421 cctcgctcgc ttttgcggac cttttttgta tccgcaccac gcccggcgca taccaaccac
     3481 agagcctttc ggggggagct tatggagtgg tcagtgtgac tttctctgtg ggcagatcgc
     3541 tcccgggcgt tggctcaccc acccaaagg
//
LOCUS       DAHBHC010000204         3557 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHBHC010000204 DAHBHC010000000
VERSION     DAHBHC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.209"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(27..1430)
                     /locus_tag="M2A35_005164"
     CDS             complement(27..1430)
                     /locus_tag="M2A35_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCB7201317.1"
                     /translation="MTSILTSKPTLNPALRSFWTTRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQSALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            complement(1396..2145)
                     /locus_tag="M2A35_005165"
     CDS             complement(1396..2145)
                     /locus_tag="M2A35_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201318.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWV
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKGTQLKTEPTLDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLMENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDA"
     gene            complement(2203..2388)
                     /locus_tag="M2A35_005166"
     CDS             complement(2203..2388)
                     /locus_tag="M2A35_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201319.1"
                     /translation="MARLNVEVIPPDSETMNGIFAEIERKYAHQPMTQKVIDEMQREA
                     ARLVRRATNTKVTFVRD"
     gene            complement(2532..2924)
                     /locus_tag="M2A35_005167"
     CDS             complement(2532..2924)
                     /locus_tag="M2A35_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCB7201320.1"
                     /translation="MKLRYKLVIVAFFVTVIGSFIWSAGHYYSKYQHEKERADEAVRN
                     AESATAITSNVLQSLQIINTVIEANQHAKQQIALESQRTQEDIKVAVADDDCAVRIVP
                     SGAVKRLHEYANGIRVGAGRSVTSQSDG"
     gene            complement(2908..3384)
                     /locus_tag="M2A35_005168"
     CDS             complement(2908..3384)
                     /locus_tag="M2A35_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCB7201321.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHRIGDLISRFKEAGGVVNEAEI"
     gene            complement(3388..>3557)
                     /locus_tag="M2A35_005169"
     CDS             complement(3388..>3557)
                     /locus_tag="M2A35_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCB7201322.1"
                     /translation="LCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGAHKE"
BASE COUNT          846 a          949 c          802 g          960 t
ORIGIN      
        1 tcgcagggct tgcttgtttc gtgccatcag gcctgtctcc ccagctgttc ccagatatcc
       61 agcgatgtat caattggcgc gaaggccata atgaatgcgt cagcaacgtt tggtgacggt
      121 atctcgcgtt ttgcgaggtc ttttttactt tcgaccatca cacgtccgtt acggtcgaaa
      181 tcacgatgag gtgttgtcag ttccagtttc agcttttcaa gcaacggaca acgagaatct
      241 atgctgatca gctcatccac aggatactgt tctccgttgt taatggcgtt aaacgtattt
      301 ctgaaacggt cagccaccag ccaccatgcc tgagccttaa gatttgcgaa aaagtctttg
      361 ttggggatgc cgttgtattc gtcatctggt tcatgcacac cagcgccggc gttaaacctc
      421 tggtaattca cacgtcgcgc gtatgcattc tcgctcttcc ggtcagcgtt aatttcagag
      481 aatttagcac cggcagacgc accaacaccg atagagtcgt aaacaatatc tgcttcacgc
      541 tccagcgccg actgataagt acgctggcag ctcttcagta attcatcttc tttggccttc
      601 cattcgtcgg cccagaaaac aacggaaccg tgacggtaaa cgttagcgca cttatctgta
      661 ccactgtcag ccacgtcaaa gccaatacgc tttcttccac tgggttcgaa atttaacgtt
      721 ttgtgcgcat ccactgcggc ttctatccag gacagtttga tgattgccgc atcatcatca
      781 gactccggaa cgccctcata cacatgttta aaaccatcag gatcacggcg tcgcgccgcg
      841 tcgataacct taagcatggt gtcagacaga aacggatttt cgtcatagtt aattttgcgg
      901 atgagagtgc cttcaggcgg atcaacaacg aagttacgcc agacgaaatc agtaacaagt
      961 ccggggttga atatgaacca gcattccgaa ccctctttac ggatcgttgg ctccagaatt
     1021 ttccactggt attcagtcag tgcgtgggct tcttccagcc acaacacgtc gatcccctcc
     1081 agtgacttaa tttcttcgat gttgcgccat aatccataaa aaacaaattc cgagccagta
     1141 acccggttaa tgattttgtt gttcagaatc cggaaacggt gccgcaggcc aaacctgtct
     1201 atctgaattt tgagcagggt atacaccgac tcttcgattt tattctggat ctgacgggca
     1261 caacaaaaac gcagggtgta tttattcgac agaaatatgg caatgccagc ggcatcccat
     1321 gattttgacg atgaccggcc accataaagc actttgttac gtgcccgcgt agtccagaaa
     1381 ctacgtaaag ccggattaag cgtcggtttg gatgtcagaa tagaagtcat tgaggtcacg
     1441 ctctccattg ccatcatcaa tacctgcatc acggcgaaga cgatcagcct ccagagacac
     1501 cttatcagtg gcggccttgc ggtaggctgt atcagcaaat atttttccta ccgtcgcaag
     1561 cgtgccaacg atggactcaa tacgaactgt attgcgcatc attgctttct cggcggcgct
     1621 gatattttcc atcagcatct tcctttcctg gtccccatta gcatcttcca gcgacaccag
     1681 ccaccgaccg atattctctg cagcgacaag gttgttagca cgaaggcgaa ataattcgtc
     1741 ctcgagcgtc aacgctttag cgtcctctat cacctcatct ttgagcagaa ggcgacgggc
     1801 ataaccaccg tgttttaatg cctgttggtt accgggttgg aatgggttag tcgggggatc
     1861 ggtacgcatt ccgcgtatcg gtttcgtatc cagtgtaggt tctgttttta gttgcgtacc
     1921 tttttgtgta aggccagtaa tggcaggctt tctgctggta cgcacttttc ttttttgcgt
     1981 accatttttg caaacctgcg taccgccact gcgtacccaa ccaagctttt tggccctctt
     2041 cctgatagcc ccttctgtaa cgccgtattt ctcgcctata tcacggaggc taaggactcc
     2101 ggcccggtat gccgattcga tggcctccca gtccggtttt gccatgaatt tttcctctta
     2161 gtgacattat cgaagcccct tatcaaagga gcttctgtaa tgtcagtccc gaacaaacgt
     2221 aaccttcgtg tttgtcgctc gccttacaag gcgcgccgct tcgcgttgca tttcatcgat
     2281 aactttttgc gtcatcggct gatgcgcata tttacgttca atctctgcaa aaatcccgtt
     2341 catcgtttcg ctgtctggtg ggataacttc aacgtttaat cgtgccattg gtttgtgctg
     2401 ccctgttttt ctcaaaagtc ctgatatcgg ccttatccct gttgcactgt gctaacgctg
     2461 acaacaacgc aacattcagg ttaaggctgg ctccccacgt aaacgggtcg ggtaaatctg
     2521 gctggggtgt ttcatccgtc agactggctg gtaacggaac gaccggcacc gacacgtata
     2581 ccgttcgcgt attcgtgcaa ccgcttaact gcgccagaag gaacgatacg aacagcacaa
     2641 tcatcatccg caacagccac tttgatatct tcctgggttc tctgtgactc cagtgcgatc
     2701 tgctgttttg catgctggtt agcctctata actgtattga tgatttgcag tgattgcagg
     2761 acgttactgg taatggctgt tgcagattca gcatttcgta cagcctcatc agcacgctcc
     2821 ttttcgtgtt gatatttgct gtagtaatgc ccggcagacc agataaaaga accgatgacg
     2881 gtaacaaaga aggcaacaat aaccagctta tatctcagct tcatttacca ccccaccagc
     2941 ttctttaaat cgggaaatca ggtcaccgat tctatgttca tactgaccgt aacctgcacc
     3001 aggtaacgac gcccagatat tgctgcaacg gtcgattgcc tgacgaatat cgccgcggtc
     3061 aatcatcggt aaagcgccac gctctttaat ctgctgcagc gctacagcat cctggctttc
     3121 tggagaaaaa tctttcagac caagttgctt gcggtaggca tcccaccagc gtgaaagaag
     3181 ctggtaacgt ccggcggctg ttgatttgag tttcggattt agcgtgacaa gtttgcgagg
     3241 gtgatcggag taatcagtga acagttcgcc accaacaata acatcataac cgtggttacg
     3301 tgtcggttgt cgcccgttat ccgttccttc tgaccatgcc accatatcaa ggaaagcttt
     3361 acgctgggaa ttaagtgtct gcattaatta ctccttatgg gcaccgaact tgttaccgat
     3421 gaccctcatt gccgcaccac gaatagcatc aacaccaatc agccccaccc caccaccaat
     3481 ggcaacagaa agtgatttag gccatccgac atactcaagc gcggatgcaa aggtcagcgt
     3541 cagagcacca caaagca
//
LOCUS       DAHBHC010000205         3533 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHBHC010000205 DAHBHC010000000
VERSION     DAHBHC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.210"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(317..2689)
                     /locus_tag="M2A35_005170"
     CDS             complement(317..2689)
                     /locus_tag="M2A35_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector HECT-type E3
                     ubiquitin transferase"
                     /protein_id="HCB7201323.1"
                     /translation="MLPTTNISVNSGVIYFESPVGSPSNEDVKVALEKWCAEGEFSEN
                     RHEVASKILNVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSIVGCSSLEVINCSIPDNVSNLSLCHCSSLKHIDGSFPETLRNSVYLNGCNSLNES
                     QCQFLAYDASQGRACLSKAELTADLMWLSANRTGEESAEQLNYSGCDLSGLSLAGLNF
                     ASVNFSDSRFDDADLSGCNLSGASLKNTSFKNSVLEECDLSFSDLTNSTISASFDRAN
                     FSGANLNSASFIGSSFKETPPDLKDAQLAGAIIVPGMELRGAILEQDNISLSVQNNSI
                     GLACCQIHIPVGSENINSVLDSSRVPDTSVMRTINSVDSKYNDEKVRSVEDLIRTLPV
                     DIFDKFNPYATLSLSNAFSIPPYLASSYVQEWLQNISGRYYNTMTDWWSKYPPGDMRD
                     GPVFKNMNDGAFLQAGIYFEKHPEKMLSCNDVFIQMAAYGMQYEGAKNQFMELYDKYL
                     QYPNVNKIAQQSDFGRGDGSAKPDWNDFNDAYNWILLSSSDDNLEMMLSLNDMYSMLS
                     SDSSTYWKSFFLFKDGEIQNTNDYTLGKLFSQSFPLFSEAYNEACSRVFLPDFLDKII
                     SDVSLKQMFIDALESDKSEIKMVDYTQQQKIGAIWKEHLDGWSLAPEHLKTITEKTNI
                     GSLSDTEKAEILFCLGAVFCKYSSSDIFGTEHESPEILRRYANGLLEEAYKLASEIFN
                     KREFYDDVRDRLQGRNNAFSCTAVLADILTSHAKGNFTDIFEQYYPLAWR"
     gene            complement(2739..3329)
                     /locus_tag="M2A35_005171"
     CDS             complement(2739..3329)
                     /locus_tag="M2A35_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HCB7201324.1"
                     /translation="MPLITSSISSNVALQSIEILREAARQNLITKDITINGQKVGIHY
                     YQRPDVFLVSGCKDGMLKTLLELGLNGSNESAKRLRSWQISSVIDTHLSFLPLGICYK
                     ILSSSFPVHSEEVFFSKEHLKCPIILDAPDNGVFIKNSVSAKVCNLYDKNAMLKLVIS
                     GSPHPLSREPITESMIMRKDECYFDPKTESFVVNNV"
BASE COUNT         1157 a          737 c          561 g         1078 t
ORIGIN      
        1 ggcagcagag tatccagcag gggaaaaaaa gttgaagcca ggttggagaa cgggaatttt
       61 catattgata cttaatatgg ttttatatat ttcagtgcca ccaattcact agcccagacg
      121 ataacggaca gcatagcttt tatccctgca aggcaggtgt tccaacgagt aacaggaacg
      181 tttaaagctc ccatacacat gtgagctaat gacaccacaa tggtaaacct tccttatcgt
      241 agcaaagctt accaagttta atgagctgtc tgccctgtga tttgcaatat atattggcat
      301 cacgtagcaa cagacatcaa cgccatgcta atggataata ctgctcaaaa atatcagtaa
      361 aatttccctt tgcatggctt gttaatatat cagctaaaac cgcagtgcat gaaaatgcat
      421 tattccgtcc ttgcaacctg tccctgacat catcataaaa ctctcttttg ttaaatatct
      481 cagatgccag tttatatgcc tcttccaaaa gaccatttgc ataacgacgc aggatctcag
      541 gagactcatg ctccgttcca aatatatcag atgacgagta cttacaaaaa acagcaccta
      601 aacaaaataa tatctcagcc ttttccgtat ctgaaagact ccctatatta gttttttctg
      661 ttattgtttt taaatgttct ggagctaaac tccaaccatc aagatgctct ttccatatag
      721 ccccaatttt ttgttgctga gtataatcaa ccatttttat ttctgattta tcagattcca
      781 gagcatctat aaacatctgc ttaagtgaga catcagatat aatcttatct aaaaaatcag
      841 gtaagaaaac acgcgagcac gcttcattat atgcttcact aaacaatgga aatgactgtg
      901 aaaataattt cccaagtgta taatcgttag tattctggat ctctccatct ttaaacaaaa
      961 aaaatgattt ccagtatgtt gaagaatcag aagataacat actatacata tcatttaaag
     1021 ataacatcat ttccagatta tcatccgatg atgacagtaa aatccagtta tatgcatcat
     1081 taaaatcatt ccagtctggc ttagcagagc catcacctct accgaaatcg ctttgctgtg
     1141 caattttatt tacattaggg tattgcagat atttatcata taattccatg aactgatttt
     1201 ttgctccttc atactgcata ccataagcag ccatttgtat aaatacatca ttgcacgaca
     1261 acattttttc tggatgtttt tcaaaatata ttccagcctg taaaaatgca ccatcgttca
     1321 tatttttaaa tacagggcca tctctcatat cgcccggagg atacttagac caccagtctg
     1381 tcatagtatt ataataacga ccggatatat tctgaagcca ttcctgaaca taagaacttg
     1441 caagatatgg aggaatactg aaagcattac tcaacgacaa tgtcgcatag ggattaaatt
     1501 tatcaaatat atccactggt aatgttctta tcaggtcctc cacagatcga actttttcat
     1561 cgttatattt tgaatcaact gaattaattg ttcgcatgac agaagtatct ggaactctgc
     1621 tgctgtcaag aacagagttt atgttctctg aacccaccgg aatatgtatc tgacaacaag
     1681 ctaaaccaat gctattattt tgcacagata aagatatatt atcttgttct aaaatagcac
     1741 ctctaagctc catcccggga actataatag cccccgccaa ctgtgcatct ttcaggtctg
     1801 gaggtgtttc tttaaatgaa gaaccaataa acgatgcgct gttaagatta gcaccactaa
     1861 aattagctct atcaaacgat gcgctgattg tgctatttgt caaatcagag aaagataaat
     1921 cacactcctc cagaacagaa ttcttgaaag atgtgttttt cagggatgca cctgacaggt
     1981 tacagccact aagatcagca tcatcaaatc tggaatcaga aaaatttact gatgcaaaat
     2041 tcagccctgc aagacttaga cctgacaagt cacatccaga gtaatttaat tgttcagcag
     2101 actcttcccc cgttcggtta gctgacaacc acattaagtc agcagtaagc tcagctttgc
     2161 tcaggcaggc acggccttga ctggcatcat atgcaaggaa ttgacattgc gattcattta
     2221 atgaattaca tccatttaaa tatacggaat tcctgagcgt ctcaggaaag gaaccatcta
     2281 tatgtttcaa agaactacaa tggcataaag ataaattact gacattgtca ggtatgctgc
     2341 aatttataac ctctaatgat gaacatccaa ctatacttaa tgaagataag ttggggggta
     2401 aacagtttat tgatttaagc tctgtacatc cattcaatac cagttctttc agagaacctg
     2461 gaagcaagtc tggtaacgtt gttattggct cactgattga taaagtctct ccattagtac
     2521 ttataacatt aagtattttt gatgcaacct catgacgatt ttcgctgaat tctccctcag
     2581 cgcaccactt ttcgagtgca actttaacat cctcgttaga tggtgaacct acaggacttt
     2641 caaaatatat tactccagaa tttacagaga tatttgtcgt gggcagcatt tatcgcactc
     2701 cttcaactta gtccactgaa aataacacca ataaaaaatt aaacattatt tacaacaaaa
     2761 gactctgttt tgggatcaaa ataacactca tcttttctca taatcattga ttctgttatt
     2821 ggctctctac ttaatggatg tggagaacca gatataacta actttaacat tgcattttta
     2881 tcatataggt tacaaacttt tgcacttaca gagttcttta taaaaacacc attatctgga
     2941 gcatctaata ttattggaca cttgagatgt tcttttgaaa aaaaaacctc ttccgaatgt
     3001 acaggaaaag aacttgaaag tattttatag catataccta atggtaaaaa actcagatga
     3061 gtatcaatta cagaggaaat ttgccatgaa cgaagtcttt tggctgactc attgctacca
     3121 tttaacccta actctaataa tgtttttaac atcccatcct tacaaccaga aactaaaaaa
     3181 acgtcagggc gttgataata atgaatacca actttctgcc catttatagt aatatctttc
     3241 gttatcaggt tttgtctggc agcttcacgt aatatttcaa tactctgcaa agcaacattt
     3301 gatgaaatgc tggatgttat caatggcata tgtcatttac cccaacaaga ctaaatttta
     3361 gaaaaaacaa ctgccgggac aactttcgcc ccctgtaacc ccaaataaga tgtcagattt
     3421 atcggcatta atttatctcc ctgttgaatt agctcctcta tcatctactt aacaaatatc
     3481 ccccggacat tgcaacacaa aaaccggagc cggactccgg ttttgtgaag ctg
//
LOCUS       DAHBHC010000206         3320 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHBHC010000206 DAHBHC010000000
VERSION     DAHBHC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.211"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..135
                     /locus_tag="M2A35_005172"
     CDS             <1..135
                     /locus_tag="M2A35_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HCB7201325.1"
                     /translation="QKGSGNFSAWVIEACRRRLINEKHAQFVPNKDKHDQSTCSDRFT
                     "
     gene            complement(217..531)
                     /locus_tag="M2A35_005173"
     CDS             complement(217..531)
                     /locus_tag="M2A35_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCB7201326.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTSVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(1060..1242)
                     /locus_tag="M2A35_005174"
     CDS             complement(1060..1242)
                     /locus_tag="M2A35_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCB7201327.1"
                     /translation="MQKLKMMLCVMILPLVVVGCASEQSVRPDVKPPPPPAWVMQPPP
                     DWQTPLNGIISPSENG"
     gene            complement(1459..1992)
                     /locus_tag="M2A35_005175"
     CDS             complement(1459..1992)
                     /locus_tag="M2A35_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7201328.1"
                     /translation="MNANIKYGLSAAVLALIAAGASAPQILDQFLDEKEGNHTAAYCD
                     GSGIWTICRGATVVDGKPVFPGMKLSKEKCDLVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            2120..2416
                     /locus_tag="M2A35_005176"
     CDS             2120..2416
                     /locus_tag="M2A35_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201329.1"
                     /translation="MNGIDPFKPISKQLDVVLPQLTKHNDLLDKVLPFYIAVTAKLSG
                     KTREEVLKYNMLALETIFGSEKAGKSPKELAESQFAYMTNIRVSEIFDKLPDIE"
     gene            complement(2441..2659)
                     /locus_tag="M2A35_005177"
     CDS             complement(2441..2659)
                     /locus_tag="M2A35_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCB7201330.1"
                     /translation="MTKNYELIVKGTRNFENKVTVILTLQDKERFAGEIFDLNINLER
                     LEGAGLDYYEVTAVKHAKQFLRDLAEKI"
     gene            complement(2664..2879)
                     /locus_tag="M2A35_005178"
     CDS             complement(2664..2879)
                     /locus_tag="M2A35_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCB7201331.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            3022..3320
                     /locus_tag="M2A35_005179"
                     /pseudo
     CDS             3022..3320
                     /locus_tag="M2A35_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          921 a          820 c          634 g          945 t
ORIGIN      
        1 cagaaaggtt caggtaattt ttcagcgtgg gttattgaag cctgcagaag aagattaatt
       61 aatgaaaaac atgctcaatt tgtacccaac aaagacaaac acgaccagag cacctgttca
      121 gacaggttta cttaaacgac ttatatatga cacaaaaagc gaccactaaa gtcgcttttt
      181 cttatggtaa caggcaataa cgctctcaga tattttttag catttttttg accgcgcgtt
      241 tccggacgta ttctgttctc ctgtcccttt atatcgtcgg aatacccgcc gctcttcaaa
      301 tcccattccc aactcagaat gtagtctgtt gaccgcttgt tttatttcgg tcaggttcac
      361 cggtgaaacc gaagtccggc gcgccttacg caaacactct gctcgtttct gtgctgccac
      421 ttttcttttc tggtcatcac ttagctgtac catcactttt gcccatcgtt cagctgctct
      481 ccggtacagt ccttttttct ccagacattc tgccacgtga tcatgtagca taagtgacct
      541 ccgattatct acagactgcc atcatgaatt taccttccct taatgaaata acaataaaaa
      601 ataaatcaca gaaaaacaat aaaacaacac acaaaaaaaa ctaaataata aacaaaaata
      661 atcaccttat tttattattt tttgagggag cgattactga acaaaaaacg ctgactatat
      721 actcaaaacc aaacaactat tctgtcaatc aggtatcatg gcaacacacg gaattaccgt
      781 gtttttgcct tctctgccca tacaatacgg gcatatactt aatactctat tgtaatattt
      841 ctatccatgc gccccactcc atttacctgt aaataatatt caaaatattt atcacagaaa
      901 tcgtttttgg ccatgaactg agcacactat aaagtccgga actgactttt tgttaaatta
      961 ccttaacgtt accagtaaca cttttcataa caaaacatca cggtatacac tgggtacgga
     1021 tatattcctg tgctccttcc agttgctttt gcattgtcat cagccgttct ctgagggtga
     1081 aataatcccg ttcagcggtg tctgccagtc ggggggcggc tgcattaccc acgccggggg
     1141 cggcggtggc ttcacgtccg gtctgacaga ctgctctgat gcgcaaccga cgacgaccag
     1201 cggcaatatc atcacgcaga gcatcatttt cagcttttgc atcagctaac tcccttgagt
     1261 atttttcatc cagtgcagca acatcgcgct gacgctgctg catgtcagta atggtggcgt
     1321 tcgccttgct gagtttttcc ttcgctttat cgagctggtc tttgtaggcg atggcgttat
     1381 cacgataata attgactgcc catgacaggc agacgatgat gcaaataacc agagcataaa
     1441 taatcgcggc gactctgctc actgatctat cccccaacag gctaatgcgc tttcctggtc
     1501 acgacgaata acctgtccat agcagttatt tgaacgtatg cggcaatcgc gcccaccatc
     1561 ttttatccac cagcgaatcg cctcgcatgc acccttacga tcaccggcat tcagccgctt
     1621 ataaaacgtc gacgggaaac acttaccggg gccaatgtta tagggacaaa atgacgcgat
     1681 acccgctttc tgtggttcag tcagtggtac tttaatattg cgctccaccc atgccagcgc
     1741 cttatcacgt tcaatggcgt taaccaggtc gcatttttcc ttcgacagtt tcataccggg
     1801 aaaaacgggt tttccatcca ccaccgtggc cccccgacag atggtccata tgccggaacc
     1861 atcgcagtat gccgctgtgt ggttaccttc tttttcgtcc agaaactggt cgagtatttg
     1921 aggagcagac gcgcctgcag caatcagcgc cagaacggct gctgacaggc cgtatttgat
     1981 attagcattc atggatattt atcaggattt atcagtttct aatctctaga tatgttaagc
     2041 actcagcccg tcagtggtgg atactggcgt atgtaataaa ggggggatgg ctgattacct
     2101 ctgataaggg aatgtaacta tgaacggtat agatccattc aaaccaatca gtaagcaact
     2161 tgatgtggta ttaccacaac tgacaaaaca caacgacctt ctggataaag tgttgccttt
     2221 ttatattgct gttaccgcaa aattatcagg taaaactcga gaagaggttc tcaaatataa
     2281 catgctggct ttagaaacaa tttttggctc agaaaaagcg ggaaaatcac ccaaagagct
     2341 ggcagaatct caatttgctt atatgacaaa tataagagta tccgaaatct ttgataagct
     2401 accagacatt gaataatttc taagcggcag taatgccgct ttatattttt tcagccaaat
     2461 ctctcaagaa ctgtttggca tgttttaccg cagtaacttc ataataatcc aaaccagctc
     2521 cttcaagtcg ctcaaggttg atgttcagat caaaaatttc accggcaaaa cgttctttgt
     2581 cctgtaacgt taagattacc gtaactttat tctcaaaatt gcgggtccct ttcacaatca
     2641 gttcatagtt tttagtcatt gtcttactct cctctcgcag ccttacgcct gtcttcttta
     2701 atcttgaaat aaaggtttgt caggtacgtc agcaagccaa acaccaggct acccaataca
     2761 cctattgcca cccactggga tggggagact ttgtccagca actgcagtag ccagtatccc
     2821 gtccccaccg ctgacgtggt gtatgacaca cctgttgtta ttttttccat ctggtacata
     2881 ccccgtctcc cgttatccgg aagctgacaa caataaaaag ccaccagtta attcctgatg
     2941 tccctgaggc ataaacgtca taatacctga ctgttatgat tgacaataat gataatgttt
     3001 atatagaaag gttcccgata tgtgttacat atcatttctc cacggggaat atccccacgc
     3061 cagcgcagac tcttttaccc gttctcttct gcgctggctc tttttttatt atgctgctgc
     3121 atttacctct ggcaccaggc tttttatctc aacaccatac gtggtacttc gtgtaaccaa
     3181 tatcataacg attaatcgac atcgaatttc tcccgtgtac aggaacagag ttaaaaagcc
     3241 ggaatcaaat cgcaggatga acatctgcca gtggctggtc gtaaaaaaaa ggccgcgcca
     3301 tgcgcagccg gaaataaagg
//
LOCUS       DAHBHC010000207         3316 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHBHC010000207 DAHBHC010000000
VERSION     DAHBHC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.212"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..216)
                     /locus_tag="M2A35_005180"
     CDS             complement(<1..216)
                     /locus_tag="M2A35_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCB7201332.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSR"
     gene            complement(247..474)
                     /locus_tag="M2A35_005181"
     CDS             complement(247..474)
                     /locus_tag="M2A35_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201333.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            585..1277
                     /locus_tag="M2A35_005182"
     CDS             585..1277
                     /locus_tag="M2A35_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201334.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            1384..2992
                     /locus_tag="M2A35_005183"
                     /pseudo
     CDS             1384..2992
                     /locus_tag="M2A35_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
BASE COUNT         1175 a          598 c          615 g          928 t
ORIGIN      
        1 acggctgaca tcatcaggga aatcgatggt gccgccgccc tgctcccgat actcattcac
       61 aatgagtgcg gcaacaacat cctgattatc tgcagccgac caggcgcgaa cggcatcacg
      121 gattttttcg tggcctggca cctgttttat ttgagaacga tttatcaccg cagtcgggct
      181 aaatccgcta gtctgttggt atgtaagtgg ttgcatagtc attgccttat cagttaacgc
      241 cgcagtttag gcggcagaat tactcgcgtt aaacaatggt gcaaggtcgg gacgaatatc
      301 tgctggttta atctttccac cagtggctga gacaattttc attacatagc gggcatcaat
      361 tccgccaccg tgtagccaac gccaaacagt gggctgggct acaccgcata gatctgccag
      421 tcgtttttga ctacctgtaa tactgattgc gagttgaatg gtttgatttg tcattatcaa
      481 ttcttattgg tattacaacg aatggataat agcaatgcgt attaatccaa tcaatagcaa
      541 aacgtgtttt gaccatcaat acgcaagcgt ataaattaaa acttatgaaa aaagaaactc
      601 ttgctgatcg cttaaaccta gcgatggaac aatctggaat gtctcaaggc gctcttgcaa
      661 aggcgtctgg cgtagctcaa cccacaatct ggagactgac aagcggcaac gcacgcggct
      721 caacaaaaat tgttgaaata gctaatgcat tgggtgttcg aacagagtgg ctctcatcag
      781 gcataggccc gatgagaaat gacggtcaac aatcagggaa gcctgctgtc aaccattcca
      841 aatactttaa gattgacgtt cttgatatag aagtcagtgc tgggccgggt gtcatcaacc
      901 gtgagtttgt agaagtccta cgctcggttg agtactcgtt tgacgatgct cgtcacatgt
      961 tcgatggcag gaaggcagaa aatatccgca tcattaacgt acgcggtgac agcatgtcag
     1021 gaacgatcga accaggtgat cttctgttcg ttgatatcac tgttaaatct ttcgacggtg
     1081 atggtatcta tgcgtttctg tacgacgaca cagcccatgt aaagcgcctg caaatgatga
     1141 aggataagct gctggttatc tctgataaca agagctactc accgtgggac ccgatcgaaa
     1201 aagacgaaat gaatcgggta tttatatttg ggaaagttat tgggagcatg ccacagacat
     1261 atagaaaaca tgggtagatt attaatgaca gatttcatca agataaccag atgatgtttt
     1321 ggtgagacat gctgacatcc tcataattta tagaatggat gtttcaagca gagcttaagg
     1381 tatatggata tgtcaaaaaa aaattttgaa ttcaaaaatt acccaatagt ctttattggc
     1441 tcaggcattt caaaaagata tttagagaac tacccaacgt gggaagagct attaaacgag
     1501 tattggaaaa taacaaatcc cacaaatgat ttctatagtt atcttttaac aataaaagaa
     1561 tcacataaaa ataacagtga taatgatatc gatcataaga tttatacaga ggcagcaagt
     1621 aaaattgaaa atgattacaa tctattgttt aggactggca aattaaaatt aaatggacta
     1681 gatgccaaga gggtttttag tgaagatatt tcaccattta aatacgctat atgccaaaga
     1741 ttttctaata acacaatcag aaaggatgta aatcttgatg aattagcatc atttaaaaca
     1801 ttaataaaaa aggccaagat aatcatcact acaaattatg atgctttcat agaaaattta
     1861 cttcaagagc aaaatgtaac tccaaaactc tatattggaa ataatggttt ttttcgaaga
     1921 tactattgga tggagtgagt tatacaaaat ccatggtgat ataaaggatc cacactcaat
     1981 aatcataaat aaagatgatt acgaaaaata tgacaacaaa tctatactca ttagtgccaa
     2041 gatactatct aacatgatta aaaatccaat agtttttctt ggatattctt taactgacag
     2101 gaatgtaaag aaacttcttt ctgatttttc ctcacaacta cctagagaag atggtcgaaa
     2161 atcagctgaa agaataattt tgattgaata taaagagaat gaacaggaag tagtaccaaa
     2221 acagattacc gatcaacagc tacaaattac atatacatct gttaaaacag ataactataa
     2281 acagatctat gatgagatca gtgctgttga tgaagggctc tcaccttatg atgttttaag
     2341 gtatcaaaga gccattaaaa cacttatcat taatgagggt gaaaaagggc acttagatac
     2401 tctattggtt tcaccttctg acttagacag acttgaagaa agtgttaaac aaggaaaaaa
     2461 tttagttgtt gctctaggcg ataaaaagta tgtattcaca caagttaaag aaataaatta
     2521 tcttgaagac tacttatttg ataaaaatga aatatctaat aaattagcca tagattttat
     2581 attaggttca gtaaactcat tacagcttcc attttcaaaa actataacct catgtaattt
     2641 gaaagagtta agtttgccag ctaaatatgt gttaaaacta aatcaacgca ttgaacgcca
     2701 cggaaaacta gacgacttgc tcaataaaat caccttagat aaaatcaatg caaataaaat
     2761 ttacaccaac atcaaagata taaaggatgc aacgttcaaa aagtacaaag aattgtctat
     2821 agtcattaag aacataaaaa atatccccaa agaggagatt gaagattatg ttaaaaaaga
     2881 agcgtttgtt caattcaagg catgcgacgt tgacaattta aaaacccagt ataggaaact
     2941 atttcttgcc tacgacttgt ttattcatgg aaatgttgaa aagataaatt aaataaacgc
     3001 cccgtacaaa gcgatggcgg ggctaataag tcatttatgt atcatttagc aactgactaa
     3061 aaaaatacac ataagaactc agactaccaa tattcgatta aatttgctgc caacctaccg
     3121 agctcaccac aacccgatgc ccctctgtca cgaggcacat taaagctgta caaatttcag
     3181 ccacaccaat aagtcttcat actctttttt ataaaaattc atttaattat caatcagaac
     3241 aagtatatca aacattatca atactaattg ctattgtcat gctattgcat cgatgttggt
     3301 atcacaaaat ttataa
//
LOCUS       DAHBHC010000208         3308 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHBHC010000208 DAHBHC010000000
VERSION     DAHBHC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.213"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..276)
                     /locus_tag="M2A35_005184"
     CDS             complement(<1..276)
                     /locus_tag="M2A35_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCB7201335.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFN"
     gene            complement(385..1938)
                     /gene="caiC"
                     /locus_tag="M2A35_005185"
     CDS             complement(385..1938)
                     /gene="caiC"
                     /locus_tag="M2A35_005185"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCB7201336.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(2012..3229)
                     /gene="caiB"
                     /locus_tag="M2A35_005186"
     CDS             complement(2012..3229)
                     /gene="caiB"
                     /locus_tag="M2A35_005186"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCB7201337.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELISKGLAKVED"
BASE COUNT          762 a          862 c          819 g          865 t
ORIGIN      
        1 attgaaaatt tcggttaatc ccgcaaatcc acccgggcca aagtcagcat ccggtgcttc
       61 gccttctgct gccgctttta aatcccagcc cgcggaaaag aacttctctc cggcaccggt
      121 aataatggcg acacgtaatt gcggatcgtc acggaaattt agaaatactt cgcccatttc
      181 aaagctggtt tttgcatcaa tagcattcgc ttttggacga tcaagggtaa tttccagaat
      241 tgatccattg cgggtcagat gtaatgattc actcattcct tttctccatt tttgcttttt
      301 cagggacgac aacatccctg caaaaaatgc atattgtttt atagcgtgat tattagctgg
      361 cagggtagtt ccctgctgtt tcatttattt cagattcttt ctaattattt tccccgagca
      421 attacgtggc agatcttttc tgatctccag ataagagggc actttaaatt tcgccatatt
      481 ttgttcgcag aagcggaaaa attcctcttc gctcaatgtt tcaccttcat tcagcaccac
      541 aaatgctttg atggcttcat cgcgaatcga atctttaata cccacaacca cgatatcctg
      601 aatttttgga tgggtggcga tgatattttc cagctccacg caggagacat tctcgccacc
      661 gcgtttgatc atattgcagc ggcgatcgac gaaataaaaa aagccctctt cgtcgcagta
      721 tccggtatcg ccggtatgta gccagccgtc ggcttccagc actttcgcag tggctttcgg
      781 gttgagaaaa tactctttga agatggtttt ccctggtacg cctttaatac agatttcacc
      841 gatctcacct gccgggagcg ggcgattgtg atcgtcgcgg atctccgctt cgtagcaaaa
      901 ccccgcccga ccaatcgacg gccagcgtcg tttatcgcca gggcgatcgc cgataatgcc
      961 cacaatggtt tccgtcatcc cataagacgt cagcaagcga acgccgaagc gttcacaaaa
     1021 cgcatctttt tcctgctccg acaagttgag ataaaacatc acttcccgca ggcggtgttg
     1081 ccgatcgttc gctgaaggcg gctgcaccat caacgtacgg atcatcatcg gaatacattc
     1141 ggtaatggtg gcgcggtact tctgcacctg tccccagaag gcgcgggcgc tgtatttctc
     1201 gaccagcaca aaggtggccc cggcagaaaa cgccgccatc gccgcagtac actggcaatc
     1261 gatatgaaac gcaggcatta ccgtcaggta gacgtcatcg tcacgcagtg cacactgcca
     1321 ggcggagtaa tatccagcga agcgcaggtt gtaatgggta atcaccacac ctttcggtcg
     1381 ggaggtggtg ccggaggtga agagaatttc cgccgtatcg tcagtcgata gcggcggtgc
     1441 atagcacaag gtggcaggtt gttgattttt cagttgagta aacgaactca cgccatcatc
     1501 agcgggaagt gccacatctg tcaggcaaat gtgccgcaat tgagtggcat cttcctgctg
     1561 aatctgttga tacataggat agaattgcgc actggtcacc agcaggcacg cctggctatt
     1621 ttgcaggatc cacgcgcttt cctcgcgtaa caggcgggcg ttaatcggca ccataatcgc
     1681 gccaattttt gccagcccga accagcaaaa gataaattcc gggcagttgt cgagatgtag
     1741 tgcaaccttg tcgcctttgc gaatccccag cgtataaaac aggtttgccg tgcggttaat
     1801 ctcctgattt aactcaagat aactataccg gttaacgact ccgccgctgg attcacaaat
     1861 cagcgccgtt ttatgaccgt aaacgtccgc aagatcgtcc cacatttgac gtagatgttg
     1921 tccgccaatg atatccattg cacctctatc catttttgtt cgtttgttat tgggcgggcg
     1981 ctagtcaggc aagccgactg acgccacgcg tttagtcctc aactttggcc agacctttgc
     2041 tgatcaactc ctgaatgtcg ttttcgctgt agccgatatt tttcaaaatg gcagccgtgt
     2101 ccatgccatg tgagggcatt ccgcgccaga tttgtccggg gttatttttg aatttcggca
     2161 tgatgttcgg ccctttgcag gtgcgaccat ccatcgtttg ccactgagtg attgattcac
     2221 gggcaacata ctgtggattg ctttccagtt ccggtacggt cagtactttg gcacaggcga
     2281 tattcagttc ggcaaagcgt tctttaactt ctgcgatggt atgtgccgcc agccaggcat
     2341 cgagtttttc ttctaccagt gggccgtaag ggcattcgat acggtggata agctgagtgc
     2401 cttccgggat ttccggcgta ccgagtagat gtgcgaggcc aatatcttta aagcactctt
     2461 caatttgggt aatgcccacc agttccatca cgatgtagcc gtcggcgcat ttatacagac
     2521 cgcaaccggc gtagtaggga tctttacctt tgctcatgcg cgggcacatt tcgccgccgt
     2581 tgaagtaatc catcatgaag tactggccca tacgcagcat cacttcatac atggcgatgt
     2641 cgatactttc gcctttaccg gtttcacgca ctttatgcag tgctgccagc gccgccgtgg
     2701 tggcggtcag gccagaaaag taatcggcgg tatacgggaa ggcaggcatt ggctggtcaa
     2761 catcaccgtt ctgaatcagg taaccactaa aggcctgggc gatagtgtta taggccggaa
     2821 gattggtgta ctcctcggtg ccgtactgac caaaaccgga caggtgagcg ataaccagtt
     2881 tcgggttgtg ctgccacagt acttcatcgg taatgccacg acgggcaaag gccggacctt
     2941 tactggcttc gatgaagata tcggtggttt ccattaattt cagaaacgct tcgcggcctt
     3001 catctttgaa aatatttaac gacagcgcgt gcaaattgcg gcgggagagt tgcgggtagt
     3061 tcggttgaac gcgaatggtg tcggcccagg cgacgttctc gatccagata acttccgcgc
     3121 cccattctgc gaacatttgc ccggcaaacg gtccggcgat ttcgataccg gagaagacaa
     3181 cgcgcaatcc ggccaacggc ccgaatttcg gcatgggtag atgatccatt atttgctcct
     3241 gaaaaattta tgtagcgcat gactgccgga tgcggcgtaa acgctttatc cggcctacat
     3301 tcgtgctc
//
LOCUS       DAHBHC010000209         3299 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHBHC010000209 DAHBHC010000000
VERSION     DAHBHC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.214"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1029
                     /locus_tag="M2A35_005187"
     CDS             <1..1029
                     /locus_tag="M2A35_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201338.1"
                     /translation="IEQAEAQLKQREQDSDRYCGREDDPQVPRQLRQSRHRRPLPAHL
                     PREIHRLEPEESCCPECGSELDYLGEVSAEQLELVSSALKVIRTVRVKKACTKCDCIV
                     EAPAPSRPIERGIAGSGLLVRVLTGKYLPLYRQSEIFARQGVELSRALLSNWVDACCQ
                     LMTPLNDALYRYVMNTRKVHTDDTPVKVLAPGRKKAKTGRIWKYVRDILQADAFTGYD
                     RLFSAEREGGALTEVACWAHARRKIHDVYISSQSATAEEALKRISELYAIEDEIRGSV
                     DAYADALYLFTKRLGKGKFMWPAVRDGKVSITRSQLAMLLDQLDWRQSKTSRFNELTM
                     LKKLLAGL"
     gene            1452..2267
                     /locus_tag="M2A35_005188"
     CDS             1452..2267
                     /locus_tag="M2A35_005188"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201339.1"
                     /translation="MSNIIKNNSQRVIKITNIRTNKYTIIYTENCTIDFYLSNKSISC
                     GKNQFIFIDKGTFFSASLRKFDKRKEPYTAIRFNSDKLRILRQLIVVLYFKDNQKINY
                     TTISNDKILTIASTNELVFFFKRLSITNDEIITLINSLLFISNFKNRDHIIHSIIASS
                     ICHFSDKVRALIETNLNRKWSVQDMAKFFHLSESATRKRLAKEKTSLKKIILQTRLNS
                     SIELIIENDLQISQISSEVGVSSTSYFIKKFKEYFGITPKKLRDYFVTQKNIS"
     gene            complement(2507..2644)
                     /locus_tag="M2A35_005189"
     CDS             complement(2507..2644)
                     /locus_tag="M2A35_005189"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201340.1"
                     /translation="MEASDIKKIKDLEDENGSLKQMFADISLENRALKDVIEKSFETS
                     L"
     gene            complement(2655..2786)
                     /locus_tag="M2A35_005190"
     CDS             complement(2655..2786)
                     /locus_tag="M2A35_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201341.1"
                     /translation="MRKARFTEHQIIAVIKSVEAGRTVKDVCRKAAISDSDLLQLEI"
BASE COUNT         1040 a          692 c          695 g          872 t
ORIGIN      
        1 attgagcagg cagaggccca actgaaacaa cgcgagcagg acagcgatcg ttactgtggg
       61 cgtgaggatg acccgcaggt tccccgtcag ttgcgacagt cgcgtcatcg tcgtccatta
      121 ccggcacatc tcccccgcga aatacaccga ctggagcctg aagaaagttg ttgcccggag
      181 tgtggcagtg agctggatta tctgggggaa gtcagtgctg agcagctgga actggtgagt
      241 agcgccctga aagtgatccg cactgttcgg gtgaaaaaag cctgtacaaa atgtgactgt
      301 atagttgaag cgccagcgcc gtcccgcccg atagagcgcg gtatcgcggg ctccggatta
      361 cttgtccgcg tgttaacggg gaaatacctg ccactatatc gtcagagtga aatctttgcc
      421 cgccagggcg tcgaactgag ccgtgcatta ctctccaact gggttgacgc atgctgtcag
      481 ttaatgactc cactgaatga tgccctgtat cgttacgtga tgaacacccg caaggttcac
      541 actgacgaca caccagtaaa agtgctggca ccgggcagaa aaaaggcaaa aacaggacgc
      601 atctggaagt atgtccggga tatcctgcag gcggatgcgt tcacaggtta cgacaggctg
      661 ttcagtgcag aacgtgaagg tggtgcactg acagaagttg cgtgctgggc ccatgcccgg
      721 cgaaaaatcc acgatgtata catcagcagc caaagtgcga cggcagaaga agccctgaag
      781 cgcatcagtg aactgtacgc catcgaggat gaaatacggg gctccgttga cgcttacgct
      841 gatgctttgt acctgttcac caaacgcctg gggaaaggca agtttatgtg gcccgcggtg
      901 cgtgatggca aagtttccat tacccgctct caactggcaa tgctcctcga tcagctggac
      961 tggcgtcagt ccaagacatc tcgctttaac gaactgacaa tgttgaaaaa actcctggca
     1021 ggattataaa aacgtccagg agtcagaaat aaataatccg atgtgttccg caagcttcag
     1081 tgctttactg caaaattcag gcgaatactg tttacggttt ttttactagt tgatactgtt
     1141 tttatcatgt gagtcacctc tgactgagag cttactcacc tggctgcgtg tccaatattg
     1201 ttggataata tcacttcggc ggcggagggt aatgagtcca ctggcatgaa aatctccatc
     1261 aaagttgcgg aagagtcact tttgcagcgt tagtaacata tagagaggat gttaatccat
     1321 actgtacgtc tataagttta acactgctgc ccaattataa taaaagaatc cgaatattaa
     1381 tacaaaaaca ttcccttgag aggggggggg ggatataata agttcgatcc aaaaaagggc
     1441 cttagaaaca catgtctaat atcatcaaga ataattcaca aagagtgata aaaataacaa
     1501 atataagaac caataaatac actatcatct atacagagaa ttgtactatt gatttttatt
     1561 tatcaaataa aagcataagc tgtgggaaaa atcaatttat ttttattgat aaagggactt
     1621 tcttttccgc cagtctgaga aagtttgata aacgcaaaga accatataca gcaataagat
     1681 tcaatagtga taaactcaga atacttagac aattaattgt agttctttac tttaaagaca
     1741 atcaaaaaat caattataca acaatatcaa atgataaaat acttacaatt gcatcaacaa
     1801 atgagcttgt tttctttttc aagaggctat ccatcactaa tgatgaaata ataaccttaa
     1861 ttaattcttt gctttttata tcaaatttta aaaatagaga tcacattatt cattctatta
     1921 ttgcttcatc tatatgtcac tttagtgata aggtaagagc attgattgaa actaacttaa
     1981 atagaaaatg gagcgttcaa gatatggcta agttttttca cttgtctgag tcggcaacaa
     2041 gaaaacgact cgcaaaagaa aagacatcac taaaaaaaat aattctgcaa acaagattaa
     2101 atagctcaat agagctcata attgaaaatg atctccagat atctcagata tcatcagaag
     2161 ttggagtgtc aagcacatca tattttataa aaaaatttaa agaatatttt ggcataactc
     2221 caaaaaaatt aagagattac tttgtaacgc agaaaaacat ttcctaaaat acggtggacc
     2281 cttttgtggt ttcaccggta tccctgccgt cgcagaatct gaagcaattt tggaaaacca
     2341 tatcatggat accgttctgc aacagcctgc agcgctgaaa taaccgactc gtcacgcgtc
     2401 gcattcggac gataatgata aaccgttctg ctcagattcc aggctccgac aggcctgacg
     2461 gatactaagc ccgaagctcg ttatctgata agtgaccagc tcgcgcttaa aggctggttt
     2521 caaagctttt ttcgataacg tctttcaatg cccggttctc aaggctgatg tcagcaaaca
     2581 tctgtttgag actcccgttc tcgtcctcaa gatcctttat ctttttaatg tcagaggctt
     2641 ccataccacc gtatttagat ttccaattgt agtaggtcgc tatcagagat agcggccttg
     2701 cggcaaacat ccttgacagt tcgtccggct tcaaccgact taatcactgc gatgatctga
     2761 tgctcagtaa aacgggcttt acgcataacg atctccttcg ttggcagaat gagtatgccg
     2821 gaagatctct aaatgtgaat ggttcgatta tgcgggataa tcacaataca acggtcacca
     2881 actatgccgt tttttatttt taagacaaca tttattcatc ttcagctttc cggaatacgt
     2941 acggaagcat gcaaggattt caataagaaa taaaaaaaaa caaaccaaaa acaataaaaa
     3001 aacaacacgt catatgatgt ggcattgaca aataaagctc acttttgttg aatgaatatc
     3061 gtaaaaggta taaggcctat aaactcacag gtatgactat tttcggcact ttatgtgccg
     3121 ctttttttat aaaatgagcc tcatatatta gacacattgc tttaaccggc gcattgtaga
     3181 gatgtgatgg acggcctttc ctgaagcaca gaagttaata agaaccgtca catccaaatc
     3241 acgcaatggc atgattgagt atctgcgggt attaaaaact tgccgaaaaa cagacatca
//
LOCUS       DAHBHC010000210         3275 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHBHC010000210 DAHBHC010000000
VERSION     DAHBHC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.215"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(66..413)
                     /gene="tnpB"
                     /locus_tag="M2A35_005191"
     CDS             complement(66..413)
                     /gene="tnpB"
                     /locus_tag="M2A35_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCB7201342.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(410..799)
                     /locus_tag="M2A35_005192"
     CDS             complement(410..799)
                     /locus_tag="M2A35_005192"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201343.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWSEGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            886..1305
                     /locus_tag="M2A35_005193"
                     /pseudo
     CDS             886..1305
                     /locus_tag="M2A35_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1305..1652
                     /gene="tnpB"
                     /locus_tag="M2A35_005194"
     CDS             1305..1652
                     /gene="tnpB"
                     /locus_tag="M2A35_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCB7201344.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFIKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHTKRTERAGIRI"
     gene            1672..3243
                     /locus_tag="M2A35_005195"
     CDS             1672..3243
                     /locus_tag="M2A35_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCB7201345.1"
                     /translation="MDTSLTHENARLRTLLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPALPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGDLSPLGCDMSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYRGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEIRGCSAEQRLAVRKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGQVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          765 a          900 c          920 g          690 t
ORIGIN      
        1 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
       61 gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca gtcgatacct
      121 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
      181 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc accggtggac
      241 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg gccggacatg
      301 ggatcgtctt ttagcgccgt ctgtactttc gcagccaggc cgttgaagcc atttctcata
      361 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
      421 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
      481 cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct gcgagcgatt
      541 ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc tctgaagaag
      601 gtaatagcag ctttccctcg ctccattgtt gtcgccattt gaacaacaga ttggcgttaa
      661 tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag gcagcaacga
      721 gctgctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg ggagtcacat
      781 ttttctgcat atctgacact ttggttccca ctacttattt ggtggacacc actttgtcta
      841 attcgtcaga ttctgaccag acggttcagg ctgtacgctt acactgaaag taccgttatg
      901 cccgaactcc ccggggtaag aaagcgtccc cggcgaccca acttccctta tgagttcaaa
      961 atcgccttag tggagcagtc actgcaaccg ggggcctgtg tggcacagat tgcccgtgaa
     1021 aacggaatca acgataacct gctcttcaac tggcgccatc aataccggaa aggggggctc
     1081 ctgccttccg gaaaaaatat gccggcactg cttcccgtga cgttaacgcc ggagccggat
     1141 aataaaattc cggcaccagt gcagatacct gaacagacaa atacaccgtc cgacagtctg
     1201 tgctgtgagc tggttctgcc ggccggaacc ctcaggctta aaggtgaact gacaccggcg
     1261 ttattacaga cacttgtccg cgaaatgaaa gggagcagcc actgatgata tctctccctg
     1321 ccggttcgcg tatctggctg gttgccggta tcaccgacat gcgaaatggc tttaacggcc
     1381 tggcctcaaa agttcagaac gtcctgaagg atgacccgtt ctccgggcat ctgttcatct
     1441 tccgtggacg ccggggtgac cagataaaag tgttgtgggc tgacagcgac ggactgtgcc
     1501 tcttcatcaa acgcctggaa cggggccgct tcgtctggcc ggtcacccgt gacggaaagg
     1561 ttcaccttac tccggctcag ttatccatgc ttcttgaggg catcgactgg aaacatacaa
     1621 aacgaacgga acgcgctgga atccgcatat aacccgttgt aaagtgagga tatggacacc
     1681 tcacttactc atgagaacgc ccgcctgcgg acactgttgc agacgcaaca ggacaccatc
     1741 cgccagatgg ccgaatataa ccgcctgctc tcactgcggg tggcggctta tgcttccgaa
     1801 atcaaccggc tgaaggcgct ggttgcaaaa ctgcagcgca tgcagttcgg taaaagctca
     1861 gaaaaacttc gcgcaaaaac cgaacgacag atacaggaag cacaggagcg aatcagcgca
     1921 cttcaggaag aaatggcgga aacgctgggt gagcaatatg acccagcact gccatccccc
     1981 ctgcgccagt cttctgcccg taaaccgtta ccggcctcac ttccccgaga aacccgggtc
     2041 atccggccgg aagaggaatg ctgcccggcc tgtggtggtg acctcagtcc tctggggtgt
     2101 gatatgtcag agcaactgga gcttatcagc agcgccttta aggttatcga aacacaacgt
     2161 ccgaaactgg cctgttgccg gtgcgaccat atcgtgcagg caccggtgcc ttcaaaaccc
     2221 attgcacgca gttatgccgg agcggggctt ctggcccatg ttgtcaccgg gaagtatgca
     2281 gaccatctgc cgttataccg ccagtcagag atataccggc gtcagggtgt ggagctgagc
     2341 cgtgccacgc tgggtcgctg gaccggtgcc gttgctgaac tgctggagcc gctgtatgac
     2401 gtcctgcgcc agtatgtgct gatgcccggt aaagtccatg ccgatgatat ccccgtcccg
     2461 gtccaggagc cgggcagcgg taaaacccga accgcccggc tgtgggtcta cgtccgtgat
     2521 gaccgtaacg ccggttcaca gatgcccccg gcggtctggt tcgcgtacag tccggacagg
     2581 aaaggtatcc atccacagaa tcatctggcc ggttaccgcg gtgtgcttca ggccgatgct
     2641 tacggtggtt accgggcgtt gtacgaatcc ggcagaataa cggaagctgc gtgtatggca
     2701 catgcccgga ggaaaatcca cgatgtgcat gcaagagcac cgaccgacat caccacggaa
     2761 gccctgcagc gtatcggtga actgtatgcc atcgaggcgg agatccgggg atgttcagca
     2821 gaacagcgtc tggcggtaag aaaagccaga gccgcgccac tgatgcagtc actgtatgac
     2881 tggatacaga ctcagatgaa aacactgtcg cgtcactcgg atacggcaaa agcgttcgca
     2941 tacctgctga aacagtggga ggccctgaac gtgtactgca gtaatggcca ggtggaaatc
     3001 gataacaaca tcgcagaaaa cgccttacgg ggagtggccg tgggccggaa aaactggctg
     3061 ttcgcaggtt ccgacagcgg tggcgaacat gcggcggtgt tgtactcgct gatcggcaca
     3121 tgccgtctga acaatgtgga gccagaaaaa tggctgcgct acgtcattga acatatccag
     3181 gactggccgg caaaccgggt acgcgatctg ttgccctgga aagttgatct gagctctcag
     3241 taaatatcaa tacggttctg gtgagccgct tactt
//
LOCUS       DAHBHC010000211         3233 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHBHC010000211 DAHBHC010000000
VERSION     DAHBHC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.216"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..743)
                     /locus_tag="M2A35_005196"
     CDS             complement(<1..743)
                     /locus_tag="M2A35_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCB7201346.1"
                     /translation="MANAWLRLWHDMPNDPKWRTISRVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTAEDLASALDVTEEVIDSILQAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKQNEGARDESRICHDMSRRVTTDKDTDKE
                     LNPTHNARVRESAPVSESNGLPLQATEPDYLEGLSEPIGKFPMTAGWNPSPDFRRRAA
                     LWGVALPEPEFTPAELAAFRDYWSDEGKV"
     gene            complement(815..1240)
                     /locus_tag="M2A35_005197"
     CDS             complement(815..1240)
                     /locus_tag="M2A35_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7201347.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIETAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVVVH"
     gene            complement(1224..1505)
                     /locus_tag="M2A35_005198"
     CDS             complement(1224..1505)
                     /locus_tag="M2A35_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201348.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            1606..2025
                     /locus_tag="M2A35_005199"
     CDS             1606..2025
                     /locus_tag="M2A35_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201349.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            2285..2503
                     /locus_tag="M2A35_005200"
     CDS             2285..2503
                     /locus_tag="M2A35_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201350.1"
                     /translation="MVGEDLVVINGQLCSKDVAAMLISKVLPTVLEVIAEKVKAGRPD
                     KEVEEAAKTVVHAATEAIILKSLVSPKP"
     gene            complement(2469..2663)
                     /locus_tag="M2A35_005201"
     CDS             complement(2469..2663)
                     /locus_tag="M2A35_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201351.1"
                     /translation="MDNKDKAWLLALAFSIRSQRETTHQEFFSEIENAENEFLSLLNE
                     RDAKKSSDSLKAWAKLGSSK"
     gene            3167..>3233
                     /gene="kilR"
                     /locus_tag="M2A35_005202"
     CDS             3167..>3233
                     /gene="kilR"
                     /locus_tag="M2A35_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCB7201352.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYV"
BASE COUNT          853 a          791 c          731 g          858 t
ORIGIN      
        1 aacactttgc cctcatcgct ccagtaatcc cggaaggcgg caagttcagc aggtgtaaat
       61 tccggctccg gcagagccac tccccacagt gcagcccgtc gtcggaaatc cggcgacgga
      121 ttccagccgg cggtcatcgg aaattttccg atgggttcgc tcaggccttc caggtaatca
      181 ggttccgttg cctgcaacgg caaaccattc gactcactga ccggagcact ctcgcgcacg
      241 cgcgcgttat gtgtggggtt taattcttta tctgtatctt tatctgtcgt gactcgtcgt
      301 gacatgtcgt gacatatgcg tgactcgtcg cgcgccccct cattctgttt tcgtaatttt
      361 tccctctcgc gctgcgctct cttgcgctct gccggggatt tcgcggtttg tgaaacgttg
      421 ccattgtcct ctttcagtac ctggcgtttt tcccatccac tgatcaaatc tccatcaagt
      481 acccgcccct gcattgcctg taaaattgaa tcaattactt cttccgtcac atcaagcgca
      541 cttgctaaat cttccgctgt gacatcaatg tgaccacgta gtgacacgcc gtgacatgtc
      601 gtgacatttc gtgacgcgct caccagaagg tggatataca cagccatcac tgttgcgatt
      661 ggctgtcctg acaccctgga aattgttcgc cacttaggat cgtttggcat gtcatgccat
      721 aatctgagcc aggcgttagc catactcacc tcttctgata ccgaatcttt ttactcacga
      781 attgccggaa gcgatccggt atgaatattg tcagtcaatg cacaaccaca atatttcccg
      841 ccggaccacc acgattcatc tgattgaaac cagcgatcgc cactgcgaca aaatcatcag
      901 cgtctctcac cagtcgttcc cgcgtctcca ccagctcccg aaaataggct gaactgtggc
      961 tgcgcattcg ggccaccagc agaggtggca ttgctgtttc gatcgctggt aacaatgcct
     1021 gaattttttt aaccgcatca ggggtgtctt tctccaccca gcggaaaatt ttctgagtat
     1081 tgcgagccag ggcttccgga tggctgtcgt catacagttc tgggaacgtc atacccagtt
     1141 caaaataagc cctggttatt tcagctgccg gaactttttc accgtccgga tgcgcccagg
     1201 cattcatcgc catgcggatg tgttcatgct tgattttcat gaatcaagct cctagaaagt
     1261 ggttgtgtta acgttttggt atcttccagc tcgggccaaa tattcatcca atcaaaaggc
     1321 cttagttgct gacgtgtaac ttcaccatta ctggctcgct caataaggac acataacgat
     1381 gcccctaaca cttgaccttt actcaatgcc tttcttagat aaccgatgct ggtaccacac
     1441 tcgcatgcaa acatacgctg ttcatctgac gaaagagaat tgagaaatat tcttaattct
     1501 tccatagcta ctccttagta aacacagcaa agaatacctg caggtaaaca aaagtcaata
     1561 cccacaggtt gtttaccttg cagtaatcgc atctattatt tacctatgga caaatatgaa
     1621 tttagacgac agcaactcat caaaattcgt gatgagaaat gcgatggtaa agcggttaac
     1681 gtggccagaa agatcgggcg cgagccttct tatgtatcaa gaatgttgta cccagagggg
     1741 aaaaagggaa aaaaacggat cgctgatgat atggtggaga ttatcgaaga gtcctttggg
     1801 ttaccccggg gatggatgga tggtatcgtt tcatcatcaa cgaacacagc ctccagttat
     1861 gaaacaaggg ttctaacgcc acgacaacgt atttttttag atctcttaga cgaactgcca
     1921 gaaagtgaag cggataactt attaaaaact cttgaagaga aaaaacagta ttacaatatg
     1981 atctacgaag aaatccgtaa aaagaaagca caaaacgcat catagctcac caaacaacta
     2041 gtcaccagtt aagacaccgc aaaaaattta cccataggta tttacttttt aaatacctat
     2101 gggtatcctt cttttcatac caactcaccc cgctccacag aatgcagggc aatacttcga
     2161 gttaccaggc agtggtcagg ggttaagtag ccagcccgag gcatacgaac atgacggcag
     2221 ttgttgattg atacaaagcg cagtagataa aacgttccgc cacccggcgt taaggggaaa
     2281 aaagatggtt ggtgaagatc tggttgttat taacggtcaa ttgtgcagca aagatgtggc
     2341 agccatgctt attagcaagg ttcttccaac tgtgctggag gtcattgcag aaaaagtaaa
     2401 ggctggtcgt ccagacaagg aagttgaaga ggcggcaaaa acagttgttc atgccgctac
     2461 agaagcaatt attttgaaga gcctagtttc gcccaagcct taagtgaatc ggagcttttt
     2521 ttagcatcac gttcattaag tagtgacaaa aactcattct cagcattctc gatttccgag
     2581 aaaaattctt gatgagttgt ttctcgctgc gatctgatgc tgaaagctaa agcaagtagc
     2641 caggctttgt ctttattatc catcggaata ccttattact ggttgtgtga gaactccagt
     2701 ataccaccga gcctgaagtg gtaaaaagac aggcgcacaa cacgaaggcg cacggaggag
     2761 gtgtttttcc cttagaaggc ttgtcgttaa atttcttcga ccgtgcgctt ccggttgtgg
     2821 caatccgcga aatggcgcgg tggtaagtat ggcgggggtt catgtaatcc ccacaggagc
     2881 accgggttgt caggttgacc atacgcctga gtgacaaccc cgccacaaca acccatgttg
     2941 attacctttt ggcgggtatc cgttttgttt tccctgtgat acccgccctt tttaaagtga
     3001 attttgtgat gcggtgaatg cggctcagcg cacgcggaac agttaaaaac aacccgtatc
     3061 tgtttaggtg atatcacgat ccggcgttaa ttgttaactg gttaacgtca cctggaggca
     3121 ccaggcaccg catcaacaaa gttcatttgt aaaaatggag ataattatga ttgcacatca
     3181 cttcggaact gatgaaatac cacgtcagtg tgtgactcct ggtgattatg ttc
//
LOCUS       DAHBHC010000212         3205 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHBHC010000212 DAHBHC010000000
VERSION     DAHBHC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.217"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..153
                     /locus_tag="M2A35_005203"
     CDS             <1..153
                     /locus_tag="M2A35_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HCB7201353.1"
                     /translation="SDENGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWE
                     NIGGMK"
     gene            330..1310
                     /gene="nleB2"
                     /locus_tag="M2A35_005204"
     CDS             330..1310
                     /gene="nleB2"
                     /locus_tag="M2A35_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HCB7201354.1"
                     /translation="MLSPIRTTFHNSANIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QLFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKEQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVD
                     AHPYYDGLGKGVKKYFNFTPLHNYNHFCDFIEFKHPNMIMNTSQYTCSSW"
     gene            1344..2357
                     /gene="nleC"
                     /locus_tag="M2A35_005205"
     CDS             1344..2357
                     /gene="nleC"
                     /locus_tag="M2A35_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HCB7201355.1"
                     /translation="MICMVLGINMKIPSLQSNFNFSAPAGYSALIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNTLRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
BASE COUNT          980 a          570 c          730 g          925 t
ORIGIN      
        1 tcagatgaga atggcattat tccggagcag gacagggtaa tcacacaggt ggtgatactg
       61 gatgcggata aaaagcagat acagtgcgtg gtaagaccgc tgcaaattct gcgtgctgac
      121 gggacgtggg aaaatattgg cgggatgaag taacccgaca gcttcacaaa accggagtcc
      181 ggctccggtt tttgttggca tgccatggtg atgtttgtta ggaaagcaaa ggtggcaaaa
      241 ctgctggagg ttttgtggtt gagtatgcca atataattaa tagattaaag agttagttgt
      301 gaagaaaata tggataaaca ggacgacgaa tgctttcacc gataaggaca actttccata
      361 actcagcaaa tatagtgcag agttcaccct gtcaaacggt ttcttttgcc ggaaaggaat
      421 atgagttaaa ggtcattgat gaaaaaacgc ctattctttt tcagttgttt gaacctaatc
      481 ctgaacgata taagaaagat gaggttccaa tagttaatac taagcagcat ccctatttag
      541 ataatgtcac aaatgcggca aggatagaga gtgatcgtat gataggtatt tttgttgatg
      601 gcgatttttc agtcaaccaa aagactgctt tttcaaaatt ggaacgagat tttgaaaatg
      661 taatgataat ctatcgggaa gatgttgatt tcagtatgta tgacagaaaa ctatcagata
      721 tttatcatga tattatatgt gaacaaaggt tacgaactga agacaaaaga gatgaatact
      781 tgttgaatct gttagagaaa gagctgaggg aaatttcaaa ggagcaggat tctttgattt
      841 ctatgtatgc aaagaaaaga aatcatgcat ggtttgattt cttcagaaat ttagccttat
      901 taaaagcagg agagatattc aggtgcacat ataatacaaa gaatcacggt atttcattcg
      961 gggaggggtg tatctatctt gatatggata tgatacttac aggtaagctt ggtacaatat
     1021 atgctcctga tggaatttca atgcatgtgg atcgtcgtaa tgatagtgta aatattgaaa
     1081 atagtgcaat aattgttaac cgtagtaatc atcctgctct acttgaggga ctttctttta
     1141 tgcatagtaa agtagatgct catccatatt atgatggttt ggggaaagga gttaagaaat
     1201 attttaactt tacaccatta cataattata atcatttttg tgactttatt gagtttaaac
     1261 accctaatat gatcatgaac acaagtcagt atacatgcag ttcatggtaa atgaatttga
     1321 taaagtttat tctgttgtaa taaatgattt gcatggtatt aggtatcaac atgaaaattc
     1381 cctcattaca gtccaacttc aacttttccg ccccggcagg atactctgct ctcattgctc
     1441 ctaatcgtgc tgaaaatgcc tatgcggatt acgttttgga tataggcaag cgaataccgc
     1501 tttccgcagc agatttaagc aacgtatacg aaagtgtaat tcgcgccgtc catgacagcc
     1561 gtagcaggct catcgatcag catacggtcg atatgattgg caacactgta cttgatgcgt
     1621 tgagccgatc acaaacattt cgtgatgccg taagctatgg cattcataat gaggaggtac
     1681 acattggttg cattaaatac agaaacgaat acgagcttaa cggagaatct gctatcaaaa
     1741 ttgatgatat tcaatcacta acctgtaacg aattatatgg atacgatgtc gggcaagaac
     1801 caattctccc catttgcgag gcaggagaaa acgagaacga agagccttat gtcagtttta
     1861 gtgttgcgcc agatactgat tcttatgaga tgccatcgtg gcaggaagga ctgattcacg
     1921 agattattca tcatgttact ggggccagcg atccatctgg agatagtaat atagagctag
     1981 gacccaccga gattctcgca cgtcgtgtcg ctcaagagct gggatggagt gttcccgact
     2041 tcaaaggata tgcagagcca gaacgagaag ctcatcttag actacgtaac ctgaataccc
     2101 ttcgacaggc tgccatgagg catgaaaaga atgagagggc tttcttcgaa agactgggta
     2161 cgatcagtga ccgatatgag gcgagtcctg atttcacaga gtattccgct gtgtctaaca
     2221 taggatacgg atttatccag caacatgatt ttccaggggt ggctatcgac gagaatttac
     2281 aggatgtaaa tcagatccaa ctgtatcatg gtgctcctta tatctttaca tttggggatg
     2341 tggacagaca caattagcgc tgattcgtct ttacagttac atagggttac tactcataga
     2401 tattaacgga gttgatgatg ggcaatcgtg caacattgta ttgtatgtaa aggcatagcc
     2461 ccgaatttta ctgttgtaag tgggcctagc gggaatggta tcgccagcat cattggcttg
     2521 gggccagtaa aaagagacgc agaaagatgc acaataggca ctacgcgtca tgcatggatt
     2581 aggtcgctca tcatttacac agtactgcta aaaaattatt aaggaaagcg cggcacttcg
     2641 tatgtaagaa cgtgtcgcgg ttggctggtg aactttcgat agtgcgagta ttgaatgatt
     2701 tccagccgtt accgatttta tataactgca gtggaattat tcaacacaca ggaaaaacgg
     2761 aataagctga gaaaaaaagc gtgcttaccc aatctggggt aaatcacgtc tgaatgacac
     2821 ctgaaaacag gtggtggcct caagaaggct tggaatattc ttctttaatg ttatgtaatt
     2881 tattgatttt tcgtgtacga ttttaagaca tttattccaa gaaaaatttt taactccttg
     2941 aattttcgac tctgtatcat cggtctcgaa aaccggagta ggggcaactc taccgggggt
     3001 tcaaatcccc ctctctccgc cactttatca atgacttatc tcccgacttc ccgccttgct
     3061 tttcctaaac agaacaatcg tagaatattc ttgaagggtt agatcgtcac tgttttctgt
     3121 tcgatactgt gacattcagc acttgattcg ctatggatct gacaggaagc tttcgagcga
     3181 aaatgtgcag ttattcagtc gtttt
//
LOCUS       DAHBHC010000213         3126 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHBHC010000213 DAHBHC010000000
VERSION     DAHBHC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.218"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..515
                     /locus_tag="M2A35_005206"
     CDS             <1..515
                     /locus_tag="M2A35_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCB7201356.1"
                     /translation="VFFGSSPNIRANWRVSLARYLRTDEGWLYLVVVIDLWSRAVIGW
                     SMSSRMTAHLACDALQMALWRRKRPENVIVHTDRGGQYCSTDYQALLKRHNLRGSMSA
                     KGCCYDNACAESFFHSLKVECIHGERFISREIMRTTVFNYIECDYNRWRRHSACGGLS
                     PEQFENQNLA"
     gene            548..1090
                     /locus_tag="M2A35_005207"
                     /pseudo
     CDS             548..1090
                     /locus_tag="M2A35_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
     gene            1279..1842
                     /locus_tag="M2A35_005208"
     CDS             1279..1842
                     /locus_tag="M2A35_005208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201357.1"
                     /translation="MRKESGLSLIESAIVLSLAAAVTAGVLYYYNSANQSKLVNDTVA
                     QIQSIVSRLNAMYTSNPSAMFQEGADVTERNKVMEAVSELTGISLMEYSEGSKVLKNP
                     MGYGIQFWGTGFRKYQIETQVKDVDTCTRLAVLDLGTVSYQGVEVYQGNTLEGTVSSI
                     QDASSYCSRVDYNSDSNPVKVRFSIKF"
     gene            complement(2098..2709)
                     /locus_tag="M2A35_005209"
                     /pseudo
     CDS             complement(2098..2709)
                     /locus_tag="M2A35_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(2462..2578)
                     /locus_tag="M2A35_005209"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            2718..2891
                     /locus_tag="M2A35_005210"
                     /pseudo
     CDS             2718..2891
                     /locus_tag="M2A35_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          829 a          712 c          830 g          755 t
ORIGIN      
        1 ccgtgttctt cggttcaagt cccaacatcc gggcgaactg gcgtgtttca ttagcccggt
       61 atcttcgcac cgacgagggc tggctgtacc tcgtggtggt gattgacctg tggtcgcggg
      121 ctgtcatcgg ctggtcgatg tcgtcgcgga tgacagcgca tctggcctgc gatgcgctgc
      181 agatggcact ctggcgacgt aagcgaccgg aaaatgtcat tgttcacacc gaccgtggcg
      241 gccagtactg ttcaacggat taccaggcct tgcttaagcg gcacaatctg cgcggaagca
      301 tgagcgccaa aggatgttgt tacgacaatg cctgcgcgga aagcttcttc cactcactga
      361 aggtggaatg tatccacgga gaacgcttta tcagccggga aataatgcgg actacggtgt
      421 ttaattatat tgagtgtgat tacaatcggt ggcgccgaca cagtgcctgt ggcggtctca
      481 gtcccgaaca atttgaaaac cagaacctcg cttagggccg tgtccacatt acatgggtag
      541 gatcaggcag cgggaacata aaggccgaat atcggtgaag gaaagcgacc agcgttggtg
      601 ttccgatggc tttgagttcg gttgcgatga tggcgaaaaa ctgcgtgtta cgttcgtcct
      661 ggactgttgc gatcgtgaag ccatagactg ggccgcaagc acgggaggtt acgataaagc
      721 gacagtgcag gatgtgatgt taggagcgat agaaaaacgc tttggcgata aagtaccgga
      781 acagtccatc cagtggctga cagataacgg ttcagcgtac agagcgcatg aaacacggca
      841 gttcgccaga gagctgaatc tgaagtcctg cacaacggca atcagtagcc ctcagagtaa
      901 tggaatggca gaacggttcg tgaaaacgat gaaggaagac tacatcgcgt tcatgccgaa
      961 accgaatgta agaacggcat tgcataacct tgcagtggcg atcgagcatt acaatgaaaa
     1021 ccatccgcat agtgcgttgg gttatcgctc tccgcgagaa tatcgacgtc agcgggtaat
     1081 gttaacttaa gatacaaccc ctgtctggaa ataagggggc aagaacactc tggattaaat
     1141 tcctgtcgac caaggatcgc agtgctgata gttgtaaaaa aaagataaaa aaacattaat
     1201 ttatagcgtt caggtcgaca gcgaccgtta aacaatttca aaataaccaa ggaatttttc
     1261 aattttagag aaaaagaaat gagaaaagaa tcaggattat ctttgattga atcagcgata
     1321 gtgctttccc ttgctgctgc agtgactgca ggtgttctgt attattataa tagtgcaaac
     1381 caatcgaagt tggttaacga tactgttgcg caaattcaaa gtatagtgtc ccgactaaat
     1441 gcaatgtaca catcgaatcc cagcgcaatg ttccaggagg gagctgatgt gactgaacgc
     1501 aacaaggtta tggaagctgt ttctgagctt acaggtatta gcttaatgga gtatagcgag
     1561 gggagcaagg ttcttaaaaa tccaatgggg tatggtatcc aattctgggg aactggtttc
     1621 agaaaatacc aaattgaaac ccaggtaaaa gatgttgata cttgcacaag attagcagtt
     1681 cttgacttag gtactgttag ctatcagggg gtggaagtat atcaaggcaa cacgttagaa
     1741 ggtactgttt catcaattca agatgcttcc agttattgct ctagggttga ttataatagt
     1801 gatagtaacc ccgttaaggt tcgattcagt ataaagttct gattgtagtt gtttctctgc
     1861 tgacatgtgt attagtcgcg acttaatctg acattgagcc ttgaaaggtt gggagttatc
     1921 ggttttgata tgagtgtcta atccttatac aaaacacgag ttaattctca tgcttcatac
     1981 taactgaacc gccccggatt tcctggaaag tgttttatct gtgaactcag gctgccagct
     2041 cattgtttcc ggtggaagca taataagctt tttctgcttc tgccggaggt gtctggcccg
     2101 ccatcccacg atgtatccgg caaacacatc aatgatgaac gccacataaa caaagccctg
     2161 ccacgaactg acggtttacg cggtcacctg cggcaacggt tttccggctg acggttgtgc
     2221 ggaccttttt accccggaga acaccggcaa gtcccataac cgccatgagg cgcaccactg
     2281 tacatctggc caccctgatt ccttcccgta acaactgacg ccagacttta cgcacaccgt
     2341 atacccggtg attttcatcg tatacgcgca gtatctcttt cttcagccag tcatcgcgct
     2401 gcgcacgggc actgcgttta tcaggatgat gtcgctgttg ctgacagtgg taatacgttg
     2461 acggggcaat atgcagttcg ctgcataccg gtccgacccc gtactgctca cgcagcttat
     2521 ccagcagtgg cattattttt tccagaggcg gtcgaactcc gccttcgcaa aataagcgga
     2581 agcctggcga aggatatcgt tactgcggcg cagttcacga ttttcgcgtt ccagctcttt
     2641 caggagctga cgttcagcgg tggtgagccc accatcaccg ccccaggtat cccgctcatg
     2701 ctggcgaacc ggaagcagtg gcgaaagtgt gctatgcaca aatggtgaag cagttcctga
     2761 gtcgtgaccc gttcgaatgc gtgctgtgtg gctgccggat ggtataccgc cgggccattg
     2821 cggggctgaa tgtatcaggg ctgaagaaaa acgcgcggga tataagcctg ctgaggtata
     2881 tccctggctg atgcaggaga ggtgcgcctg caatccggga aaagtataaa aaacggccca
     2941 ttacggtata aaaaccgacc atacatagag agaaaacacc ggagtggtgg gaaaagcaga
     3001 aaagctcacg tggcataagg cgcggacagg ggtatcgctc agtttttcaa tttcctatac
     3061 gtgaaccatc gacatctggc agtgccagag ctgccggata atcaatggca acaggcagcg
     3121 atgcag
//
LOCUS       DAHBHC010000214         3086 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHBHC010000214 DAHBHC010000000
VERSION     DAHBHC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.219"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..201)
                     /gene="rrrD"
                     /locus_tag="M2A35_005211"
     CDS             complement(<1..201)
                     /gene="rrrD"
                     /locus_tag="M2A35_005211"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCB7201358.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYT"
     gene            complement(201..416)
                     /gene="essD"
                     /locus_tag="M2A35_005212"
     CDS             complement(201..416)
                     /gene="essD"
                     /locus_tag="M2A35_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCB7201359.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1005..2102
                     /gene="ompC"
                     /locus_tag="M2A35_005213"
     CDS             1005..2102
                     /gene="ompC"
                     /locus_tag="M2A35_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCB7201360.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(2292..2675)
                     /gene="quuD"
                     /locus_tag="M2A35_005214"
     CDS             complement(2292..2675)
                     /gene="quuD"
                     /locus_tag="M2A35_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCB7201361.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2761..2901)
                     /locus_tag="M2A35_005215"
     CDS             complement(2761..2901)
                     /locus_tag="M2A35_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCB7201362.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2898..>3086)
                     /gene="rusA"
                     /locus_tag="M2A35_005216"
     CDS             complement(2898..>3086)
                     /gene="rusA"
                     /locus_tag="M2A35_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCB7201363.1"
                     /translation="IECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
BASE COUNT          933 a          708 c          604 g          841 t
ORIGIN      
        1 ggtatacgtt ttaccgagca tgatgtcttt tcctgtatgc ccgtgacata cagtccatac
       61 accaacaata tctttgtatg gtatgtagct gacaccttcc agaccatcgt taccacttgg
      121 gccagtgatt aacactgatg ctatagcaat tgctccgcca ccaatagcag cagcaacggc
      181 ttttcgtaat gatggaggca ttattcacct ctcgcagcct tgcgcttatc ttctttaatc
      241 ttgaaataaa ggtttgtcag gtacgtcagc aggccaaata ccaggctacc cagcacacct
      301 attgctgccc actgtgaggg cgtgacttta tcgagcagct gtaaaaacca gtaaccggca
      361 ctacctgctg aggtgccata ggcgacaccc gttgttaact tatccatgga tttcataacc
      421 ccacctcgca gacaaagcgg gtgtaaattg agggaataca acgtatcgca aaaaagcaga
      481 aacgtaacag actcggagtc agtgaataac tcaggtattg agttatcagc taatatcgag
      541 actcaaaaaa tggaaaagcc agctcgacgg cgggtttaag ctgtgtgacg aagtaaccac
      601 tcttaacagc ataaccaatt ttttacgtac gtaaaccact gaatgatatt tatgagaatg
      661 ctaccgagtg ttcaaaacac caccacaaat acataagaaa acctcaacaa ataaccaata
      721 aataatttcc ggcgttattt ttagttaatt taaattaaac caccgaatta tagacttccc
      781 ataaataaca gccattaata taaattagct aataggttta tttttgttca aataagagcc
      841 ataaataggt ttcgatagaa aaagttcaga taaaaataga gatctacttc acaaattaaa
      901 tgagaaacta aaacttacat cttgaaataa tcacattgat tagatgaata tttatcgcgc
      961 agtgacatca ttttttaata atagttcaaa aaaaagggct cacgatgaaa aaattaacag
     1021 tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag gcagctgaaa
     1081 tttataataa agacagtaac aagctggatc tgtacggaaa agttaatgcc aagcactact
     1141 tttcctctaa cgatgcagat gatggtgata ctacttatgt tcgtctgggc ttcaaaggcg
     1201 aaacccaaat caacgatcag ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca
     1261 accgcgctga atctcaaggt tcctccaaag acaaaacccg tcttgcattt gcaggcctga
     1321 aattcggtga ctacggctca atcgattacg gccgtaacta cggtgtagca tacgacatcg
     1381 gtgcgtggac tgacgttctg ccagaattcg gtggcgatac ctggacccaa acagatgtgt
     1441 tcatgactgg tcgcaccact ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg
     1501 ttgatggtct gaactttgct gctcagtacc aaggcaaaaa cgatcgtagc gatttcgata
     1561 actacaccga aggtaacggt gatggcttcg gtttctctgc tacctatgaa tacgaagggt
     1621 tcggtatcgg tgcaacttat gcgaaatctg atcgtaccga cactcaagtt aatgcaggga
     1681 aagttcttcc tgaagtattt gcttccggta aaaatgcaga agtttgggcc gcaggtctga
     1741 aatatgatgc taacaacatt tacctggcca ctacctattc tgaaacccag aatatgactg
     1801 tatttgctga tcacttcgtt gctaataaag ctcaaaactt cgaagctgtt gcacaatatc
     1861 agttcgattt cggtctgcgt ccgtccgttg cttacctgca atctaaaggt aaggatcttg
     1921 gagtatgggg cgatcaggac ttagtcaaat atgttgatgt aggtgcaacc tattacttca
     1981 acaaaaatat gtctactttc gttgattaca aaatcaacct gcttgacaaa aatgacttca
     2041 ctaaagcact cggtgtaagc actgatgaca tcgttgctgt aggtctggtt taccagttct
     2101 aatctgatta cgaaaaagat atgttgcggg aggctttgcc tccccaacat ataagtggct
     2161 ccctcaagcc acttccttta ggagcacaac cttgcttcta actatataaa ccttctgtta
     2221 tatattaccc tttatttttg ggggcgttgc aacgccccat ttttaataat ttttagtaaa
     2281 caattggcat attaattaga gttattaaca acgatatcca tctctaaccg gatatctaat
     2341 gccattaaca tcccttcaat tatgccctca gccttctgta accttttccc gatataacca
     2401 tcagagcagc aatgcttacc tgccagtgac atgaatgtca taccgactac ataataatct
     2461 actaataaat cgtgcaaatc gctgttgttc tttttcagac gggccatgca cccgcaaatg
     2521 atcatcgcgt catcgtcaca acattgcggg cgagatttta cttttgaagg aattaatccc
     2581 ttaaaaccgg cggcaatgga cgaccaggtc acatcttcat gattattagc cgcccacgct
     2641 ccccaacgct caagaaccat ctgaatatca cgcatcaact tactccacaa aaatcagacc
     2701 agaacgccaa ttacaagcaa aaatcaacaa aacagtatta gttgattgtt atctctgact
     2761 tcatactcct gctcctgtca gggttttggc gtaattcttc agtattcggt aatcggtcaa
     2821 aacagaaccg gggaaacgat ataagcgcag acgcccccag cggtggcgaa gaagttctgc
     2881 catattaaac tcaaacatca ttcattcccc atttcggtga tggtcagttc cagcctccca
     2941 cctttggtaa caggcatctt cacaacgcgg taatcaacga cctgagcatc atccagccag
     3001 aaacctgctt tagtgagtgc gtcaaaagcg gctttttgca gattatccag gtcacggcga
     3061 cggcgatccg gcatgtggca ctcaat
//
LOCUS       DAHBHC010000215         3069 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHBHC010000215 DAHBHC010000000
VERSION     DAHBHC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.220"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(43..408)
                     /gene="traA"
                     /locus_tag="M2A35_005217"
     CDS             complement(43..408)
                     /gene="traA"
                     /locus_tag="M2A35_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCB7201364.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(441..836)
                     /gene="traY"
                     /locus_tag="M2A35_005218"
     CDS             complement(441..836)
                     /gene="traY"
                     /locus_tag="M2A35_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCB7201365.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            complement(935..1624)
                     /gene="traJ"
                     /locus_tag="M2A35_005219"
     CDS             complement(935..1624)
                     /gene="traJ"
                     /locus_tag="M2A35_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HCB7201366.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIHYL
                     YEKAMCIINTR"
     gene            complement(1811..2194)
                     /locus_tag="M2A35_005220"
     CDS             complement(1811..2194)
                     /locus_tag="M2A35_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCB7201367.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            2608..>3069
                     /locus_tag="M2A35_005221"
     CDS             2608..>3069
                     /locus_tag="M2A35_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCB7201368.1"
                     /translation="MKKWMLAICLMFISGICEAADCFDLAGRDYKIDPDLLRAISWKE
                     SRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYEIKPEHLTTDPCMNIYTGAYYLAI
                     AFKKWGVSWEAVGAYNAGFKKSERQNRRRLAYASDVYRIYTGIKSSKGIQVP"
BASE COUNT          938 a          656 c          566 g          909 t
ORIGIN      
        1 tcttaagttt attctcgtct cccgacatcg ttttatttcc tgtcagaggc caacgacggc
       61 catacccaca gcaataaata cagagatgat ggcaaaaccg gccaggaact tgacgttttt
      121 ggtcatcatg tacatgaccg caccgaccag aacttcagcc agaacaaccc atttaacaac
      181 actggagtcc ttaccgaagg tcgccttaac cgtggtgtta ccacttgcca tcaggtcctg
      241 accactgctg ccagcggcca tcgccagttg cgggaagaac atcatcagaa cagcagccgg
      301 gatcacagcg cgaaccaggc gaagcatatt cagacgagtg aatttagaaa aaaacgactt
      361 ctttttgacg ggcgcagaag caccctgaac acttaaaaca gcattcataa gttacctcaa
      421 tttcggataa atgaataaaa ctagagtgta ttaaatgtta tatcttcctc tttatctgcc
      481 tccctgaata tctccgagtt ataaaaatca ggaaacttga tcaaatgatc aataactctg
      541 tctgcagcct catctgtttt acaccatcca ctacgatttt tggctgcaat aagtagctgg
      601 ttagtttcat catccagacg cattgtcaga tacttaacca gcgacttacc atagatattc
      661 cctgccctgt taaactttgg atagcggtga agatgatctt taagtcttat tactgcctca
      721 aacgatcgac ttcttccgct tctgttactt gcctcaatta atagactttc cacatctaca
      781 ggtaatttta gttttaccat cttacctgtt gcagaacgtg taccaaatct tttcaataac
      841 acctcccgct gtttatcttc tggttactac ttatgtttgc agaataatca gaaaaggtaa
      901 atagacggaa agtctatatt ttattaacac tttgttaacg cgtatttatg atacacatag
      961 ccttctcgta caaataatga ataaactttt tattgtagag taagatgatt aactcatctt
     1021 tattagaaac agagaagtag tcgcatatca gagacgcgtg tttctttgat gtggagatgg
     1081 atatgtttaa tattcttgct atagcattat gactgattcc ggtgctatat aagtaaaaga
     1141 catcatttac atatgatgat acagaagaat catcactcat tcttctgatt atatttctga
     1201 tatcatcact gacaatcgtt ttttgattat aactgtcttt atgacgaaca tgagcagcat
     1261 cataaaattg ccacagaatg atatttctgt gtccattaag aaatgactgg acagatattg
     1321 tccagaatct gttctgaata aaaacatcct ctacaagatt aagatgcgta tgctcattcc
     1381 tgtatgcttc catctctgtg acagagatca attcacaaaa atctctctgc gacagaagcc
     1441 atttttcagc actttgatct tgtgtaagaa atgactcatg aaacagcgta ttgcaaaaaa
     1501 taaattttcc agtttcgtcc ctgatacagg ctggatttgg ataatcctga ataactgccg
     1561 tcagattttc cagcagatct atttgacgag catgtttttg ttgaatacga tccatcggat
     1621 acataggaac ctcctcacaa aggattctat ggacagtcga tgcagggagg cgtgaactcc
     1681 ctgcatcggc gattttcaaa ttcaaatttt aacgtggcat taaccacgta attaatatac
     1741 taattatctt tcgtactgtc acctttttaa atcataaaaa ctctgatatt tgaacgaagt
     1801 caaatttctt ttattcatca tcattttttg gaaaaaatcg ttccatctca gatgatacct
     1861 tctccctgat atcttcaacc atattggcat attcaaactt tggatttccg gagacatgag
     1921 gactgagaga ctcaataccc aaaattttcg ctactgatga ttgtgttttt acaacgcatt
     1981 caagaagcaa tttattaaac tcagtctgat taaatgcaga ctctttacgc tccatctgag
     2041 cctcatgtac acgaagcccc agttcaagaa gcattgaagc tgttgctgaa aaactgacat
     2101 ctttttccct tgccccttcc tgtcgacgct tctcaataat cgcatttatt ttttcatagg
     2161 catcattgct gatatacagg ttcaccttag ccattagaga ttaccttttt tgaacataaa
     2221 ataaagttaa tttcgaaaca ctaaaattca taatgcaaac agggacgcac cgctagcagc
     2281 gcccctagcg gtatcctata aaaaaacaca ccgcgccgct agcagcaccc ctaatataaa
     2341 ataatgtttt ttataaaaat agtcagtacc acccctacaa agcggtgtcg gcgcgttgtt
     2401 gtagccgcgc cgacaccgct tttttaaata tcataaagag agtaagagaa actaattttt
     2461 cataacactc tatttataaa gaaaaatcag caaaaacttg tttttgcgtg gggtgtggtg
     2521 cttttggtgg tgagaaccac caactagttg agccttatag tggagtaggt taaattattt
     2581 ccggataatg tcaccagagg taaaaaaatg aaaaagtgga tgttagcaat ctgcctgatg
     2641 tttataagtg ggatctgcga agccgccgat tgttttgatc ttgcaggccg ggattacaaa
     2701 atagacccgg atttactgag agcgatatca tggaaagaat cccgttaccg ggttaatgcc
     2761 attggtatta atccggtaac gggatatggc agcggattga tgcaagtaga ctcccagcat
     2821 tttaacgaac tggcccgcta cgaaattaag ccagaacatc tgacaacaga tccctgcatg
     2881 aacatttata caggtgccta ttatctggca atagcattta aaaaatgggg cgtatcctgg
     2941 gaagccgttg gtgcatacaa tgccggattc aaaaagagtg aacgccagaa ccggaggcgt
     3001 cttgcctatg catcagacgt ttaccggatc tataccggga taaagagcag taaaggcatc
     3061 caggttccg
//
LOCUS       DAHBHC010000216         3008 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHBHC010000216 DAHBHC010000000
VERSION     DAHBHC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.221"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            33..221
                     /locus_tag="M2A35_005222"
     CDS             33..221
                     /locus_tag="M2A35_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201369.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            230..910
                     /locus_tag="M2A35_005223"
     CDS             230..910
                     /locus_tag="M2A35_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCB7201370.1"
                     /translation="MGTATLILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKEW
                     LAWDARAKKGTVVTTDKWDVIVAVIKRAHKYGKRIVIVDDFQYVMSNEFMRRSEEKSF
                     DKFTEIGRHAWEVIKAAQDAPDDLRVYFLAHTEETPMGRVKMKTIGKMLDEKITVEGM
                     FTIVLRTLTRDDQFFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGINNV
                     HQIKENAA"
     gene            907..1494
                     /locus_tag="M2A35_005224"
     CDS             907..1494
                     /locus_tag="M2A35_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCB7201371.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAHIITITEAEIKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCAGIGQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKAEGKQSETI
                     ANMVASLKDKDNRSKNVSHNHADYYGYSQNDYPPF"
     gene            1518..1811
                     /locus_tag="M2A35_005225"
     CDS             1518..1811
                     /locus_tag="M2A35_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCB7201372.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            1822..1989
                     /locus_tag="M2A35_005226"
     CDS             1822..1989
                     /locus_tag="M2A35_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCB7201373.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGNQ"
     gene            1986..2210
                     /locus_tag="M2A35_005227"
     CDS             1986..2210
                     /locus_tag="M2A35_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201374.1"
                     /translation="MSGKRMTNREIVDAAIKLAGDFYSMMGYTHRPGFKYWESPHPQE
                     QLVFQMSCRAFEVIRGSDVMDAVADLEDEE"
     gene            2207..2410
                     /locus_tag="M2A35_005228"
                     /pseudo
     CDS             2207..2410
                     /locus_tag="M2A35_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            2468..>3008
                     /locus_tag="M2A35_005229"
     CDS             2468..>3008
                     /locus_tag="M2A35_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCB7201375.1"
                     /translation="MERNQQYIKRRDQENEEIALTVGKLRVELEAAKKRIAELEAEPV
                     SQTYNLPELIEGMEVSIDVSTCDADLGNRYFGTVTEALELDTAKNGYILLVQDAEPNF
                     DINGNSPVTPDGWISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDWSRNKWWAITD
                     DGEEPASKVTHWMTLPEPPQ"
BASE COUNT          952 a          641 c          737 g          678 t
ORIGIN      
        1 gccgtcgaag cctacagaaa atggaggtca aaatgattcc ggtagagctg gcgaaaaccc
       61 cagagttaag ccgattaaaa agagagtatc acattgctga ggctcgttac tggcgtaaag
      121 cgggagataa atcaaagaaa caactttgct tatggcaggc acaaagagag cgcatgaatg
      181 agcgcgagtt tctttcctcc ccatccgaat taccattctg aggcaaatta tgggaactgc
      241 gacattaata ctcggtgagt ctggcaccgg aaaatcaacc agcatgagaa atatcaatcc
      301 agaggaagca atacttataa aaccaatagg caagccgcta ccatttaaat caaaagaatg
      361 gctggcatgg gatgccagag caaaaaaagg aaccgtagtt accactgaca aatgggacgt
      421 aatagttgcc gtaattaagc gtgctcacaa atacgggaaa agaatcgtta ttgttgatga
      481 cttccagtat gtgatgagca atgagtttat gcgccgctca gaagaaaagt cgtttgataa
      541 atttactgag ataggccgcc acgcatggga ggtgattaag gctgcacagg atgcgcctga
      601 tgacctgaga gtctattttc ttgcgcacac cgaagaaacc cctatggggc gcgtgaaaat
      661 gaaaactatc ggcaaaatgc tggacgagaa aatcactgtc gaaggcatgt ttactatagt
      721 tcttcgcacc cttacccgcg atgaccagtt ctttttcacc acgaaaaaca acggtgcaga
      781 cactgtcaaa tccccaatgg gaatgtttga ttccaatgag attgataacg atctctcttt
      841 cgtcgatgcc actgtttgtg attactacgg catcaataat gttcatcaaa ttaaggaaaa
      901 cgccgcatga gcaacgtgat ttttacttat aacgaagaag cagcactgac cgcagggcaa
      961 ggtggtttta ttaacgaaac tggcgctcat atcattacca ttactgaagc agaaataaag
     1021 caatcagaaa aaggagccaa atttattgag ttttctggcg aatccgacga cggacgtaaa
     1081 atccaatatc ttagcgtctg tgttcagaaa aatgacggaa cggaaaacaa atttggcgca
     1141 aatgtcgttc acgccatgat ggggtgtgcc gggattggac aattaacgca acatatggtt
     1201 tccgccagta aatttgttgc gcctgaattt catggaaaga aaatcgggtt agtgctccag
     1261 aaagtattaa ccacaaacaa aaagactggc gcagatagtt accagatgga aatacgcatc
     1321 ccgtttattg cacaaacagg tcaaaccctt aaagaaaagg cagaaggcaa gcaatcagaa
     1381 actatcgcca acatggttgc cagcctcaaa gataaagaca atcgctctaa aaacgtaagc
     1441 cataatcatg cagattatta tggttacagc cagaacgatt accctccttt ctgattactg
     1501 aaaataaggc tcccattatg ccagcgcctc tgtatggtgc ggacgacgcg cgccgctgtt
     1561 ccggcaattc cgtatcggag gtgctggata aattcagaaa aaactacgat cggataatgt
     1621 cgctaccgca ggaaacgaaa gaggaaaagg aatttcgcca ctgtatatgg cttgcagaga
     1681 aagaagaacg cgagcgaatt taccagacat caatccgacc attccgcaaa gccacatata
     1741 cccacttccc tgaaattgac ccgcgcctgc gtaattaccg ctcacgttat ggcgctatca
     1801 gtaatgactg aggaatttac catgagagga cttgcataca atcccggcat tcttccggca
     1861 gaaatgatta ttcgccaacg cgtaaagcca atgccatcga gagaggaatt acttaagagg
     1921 aattcttttc cgtcagtaaa tcaaaacaaa tatctgaatg cgatgtggcg caaaggaggc
     1981 aaccagtgag cggaaaaaga atgactaaca gagagattgt tgatgccgcc attaagcttg
     2041 ctggtgattt ttattcaatg atggggtaca cgcatcgccc aggcttcaaa tattgggagt
     2101 ctccgcaccc gcaagagcaa ctggtatttc aaatgtcctg ccgtgctttt gaggttattc
     2161 gcggttctga tgtgatggac gccgttgccg acttggagga tgaagagtga gcaagattga
     2221 ctatcaggca ctgcgtgaaa aggcagagaa agcaactaaa ggaagctaca tcgtagggca
     2281 tacatctgtt aaccaacacg gcaatttaac aggagttttt gtttgtcaaa aatggaaagg
     2341 agaacccggt ggcgtgattg cggaatgtca tgttaactgc ctgattgaaa cagatgctca
     2401 ggcttatgca aacgctgaat tcatagcaga ggctaacccg gctaccgtgc tggcactgct
     2461 ggatgagctg gaaagaaacc agcaatacat caaacgccgc gaccaggaga acgaggaaat
     2521 tgcgctaacg gtagggaagc tgcgtgttga gctggaagca gcaaaaaagc gcatagcaga
     2581 actggaagcc gaacccgtaa gccaaactta caacttgcca gaattaatcg agggcatgga
     2641 agtttccatt gatgtaagca cttgtgatgc tgatttaggt aatcgctatt tcggcaccgt
     2701 caccgaggcg ttagaacttg atactgccaa gaatggttac atcctcctgg ttcaggacgc
     2761 agagccaaac ttcgatataa atggcaactc tccggtaact ccggatggtt ggataagctg
     2821 tagtgagcga atgccaaacg ataaacagta tgtttggtgt tgggggaagt cttacggctg
     2881 gactgagtgc gataccttcg aagggtatta cgattggtcg agaaacaaat ggtgggcaat
     2941 tactgacgat ggggaagaac cggcatcgaa agtaacccac tggatgacgc taccggagcc
     3001 accgcagg
//
LOCUS       DAHBHC010000217         3000 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHBHC010000217 DAHBHC010000000
VERSION     DAHBHC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.222"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..85
                     /locus_tag="M2A35_005230"
     CDS             <1..85
                     /locus_tag="M2A35_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCB7201376.1"
                     /translation="ATKEDKQRISEILQETWESADDWFVSE"
     gene            375..629
                     /gene="bssS"
                     /locus_tag="M2A35_005231"
     CDS             375..629
                     /gene="bssS"
                     /locus_tag="M2A35_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCB7201377.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            744..1862
                     /gene="solA"
                     /locus_tag="M2A35_005232"
     CDS             744..1862
                     /gene="solA"
                     /locus_tag="M2A35_005232"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCB7201378.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1910..2023
                     /locus_tag="M2A35_005233"
     CDS             1910..2023
                     /locus_tag="M2A35_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCB7201379.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2284..2850
                     /locus_tag="M2A35_005234"
                     /pseudo
     CDS             2284..2850
                     /locus_tag="M2A35_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            2854..>3000
                     /locus_tag="M2A35_005235"
     CDS             2854..>3000
                     /locus_tag="M2A35_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201380.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLY"
BASE COUNT          765 a          713 c          683 g          839 t
ORIGIN      
        1 cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc
       61 cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg
      121 gcttttttat tttacttaca aattttgagt acaaaatttc tacacttcct caaaataatt
      181 gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa
      241 agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat
      301 actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga
      361 attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt
      421 gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc
      481 caaataagtc cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg
      541 ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt
      601 tcccggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct
      661 caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa
      721 accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg
      781 taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg
      841 cccatatgcc accgcatcaa cacggcagcc accatggcga tacgcgatta attcgccatg
      901 cttatggtga aggcgaaaag tatgtcccgc tggtcctccg cgcgcaaacg ctgtgggatg
      961 aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg
     1021 gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca
     1081 acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg
     1141 acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta
     1201 aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca
     1261 ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac cgttgacggt gagtatcagg
     1321 cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg
     1381 tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga
     1441 ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg
     1501 cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg
     1561 atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc ccgttcttgc
     1621 gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt
     1681 cgcctgacga agattttatt atcgataccc tacccggcca cgataataca ctgctcatta
     1741 ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt
     1801 ttgcgcagga caaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat
     1861 aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct
     1921 gttgcactat ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg
     1981 attgttggca attatggatc tgatctatgt gttttatttt tagttttccg cactgacata
     2041 aactgacaaa tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct
     2101 gcttctacag cactcaccag acatctctga tgctgaaccc actaaactta tgaagattct
     2161 ttcattatta attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg
     2221 tgttgcaatt cattctccga cacgccattt tctttctctc gcaaattcgc attggatgca
     2281 cttatgtcat tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg
     2341 ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt
     2401 tattacgatg gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta
     2461 atgatggggc tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg
     2521 ccgagttatt cgcccatgac tcgtcttgcc gcataggctg gacatctcgc attgtaccta
     2581 ttgctgttcg ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc
     2641 agcgtttttg gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac
     2701 tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc cgttatgcac
     2761 ggatttatgg ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg
     2821 ggaaagtcgt catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc
     2881 ttcgcgtccc tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa
     2941 gaaggtcagc acgcctttgt taatttccgc atccagcacc ttggctatag ctggttatac
//
LOCUS       DAHBHC010000218         2929 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHBHC010000218 DAHBHC010000000
VERSION     DAHBHC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.223"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            106..219
                     /locus_tag="M2A35_005236"
     CDS             106..219
                     /locus_tag="M2A35_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7201381.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQSYF"
     gene            289..1275
                     /locus_tag="M2A35_005237"
                     /pseudo
     CDS             289..1275
                     /locus_tag="M2A35_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            1295..1915
                     /gene="traX"
                     /locus_tag="M2A35_005238"
     CDS             1295..1915
                     /gene="traX"
                     /locus_tag="M2A35_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HCB7201382.1"
                     /translation="MTTDNTNTTRNDSLAARTDPRLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQMLTWCETRSGWRSVGAILLLAVIPALNPATSDAAA
                     VAGLVMTVLTAGLVSCAGKSLPRFWPGDFFPAFYAGHLAVLGVLVL"
     gene            1970..2530
                     /locus_tag="M2A35_005239"
     CDS             1970..2530
                     /locus_tag="M2A35_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCB7201383.1"
                     /translation="MTEQKRPVLTLKRKTEGDTPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            2664..2876
                     /locus_tag="M2A35_005240"
     CDS             2664..2876
                     /locus_tag="M2A35_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201384.1"
                     /translation="MNGFRNSSRNGQVWRWQLAGKRAVTLEVRGLWMEAAEAWQRAAY
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          715 a          721 c          948 g          545 t
ORIGIN      
        1 atatcggagg gaacaaaagg caggtccccg caaaacgtca aaaccgcggc tcccgaaact
       61 caaaagatga tgggggctga aaacatcaac agaaggagac acatcatggc agttcgtgga
      121 attaacaagg tcattcttgt cggtcgtctg ggaaaagatc cggaagtccg ttacatcccc
      181 aacgggggcg cagtggcaaa cctgcagtcg tatttttaat tattacagcg taccggagga
      241 atcacgcatg gtactcagtc gtcaggaatt actccggctt atgcgtctga aggcacggaa
      301 ggtggccgga aacagatggc cggctttgag tcagcctacg ttgccctgtc gcgtatgaag
      361 cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat caacaatgcc
      421 gtacagaaag gaactgccca cgatgtgctg gagccgaaac cggaccggga ggtcatgaat
      481 gcagagcggc tgttcagtac ggcgcgggag ctgcgggacg tggcggccgg ccgtgccgtt
      541 cttcgtcagg cggggctggc cgggggggac agtccggcac ggtttattgc accggggcgc
      601 aaatatccgc agccgtatgt ggcactgccg gcttttgacc gtaacggcag gtcagccggt
      661 atctggctga acccactgac cacggatgac ggaaacggac tgcggggatt cagcggtgaa
      721 ggacgggtga aaggcagcgg ggatgcgcag ttcgtggccc tgcagggcag ccgtaacgga
      781 gaaagcctgc tggctgataa tatgcaggat ggcgtccgga ttgcccgtga taatcctgac
      841 agtggtgtgg tggtaagaat cgccggtgaa ggccgtccgt ggaatcccgg tgccatcacc
      901 ggtggccgcg tgtgggggga tatcccggac aacagcgtcc agccgggagc cggaaatggc
      961 gaaccggtca cggcagaggt actggcacag cggcaggctg aagaggccat ccgccgtgaa
     1021 acggaacgcc gcgcagatga aattgtccgt aaaatggcag agaacaaacc cgacctgccg
     1081 gatggcaaaa cagagcaggc tgccataact gaacgggaag ccgcgctgcc ggagagtgtg
     1141 ctgcgtgaat cacaacggga gcgggaagcg gtccgggagg ttgcccggga aaatctgttg
     1201 caggagcgac tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc
     1261 ctgggcggag actgatacag gaagacaaac gctgatgacg acggataaca caaacacaac
     1321 acgtaacgat tcactggctg cccggactga tccccggttg cagtcgttgc tggtctggtc
     1381 acccggacag cgggatatta tcaaaacggt ggcactggtg ctgatggtac tggaccatgc
     1441 aaaccgcatt ctgcatctgg accagacgtg gatgtttctg gccgggcgtg gagcctttcc
     1501 gctttttgcc ctggtatggg ggctgaatct gtcccgccat gcgcatatcc ggcaaccggc
     1561 cattaaccgg ctgtggggct gggcggtgat tgcccagttc gcgtattatc tggccggatt
     1621 tccctggtat gaggggaata tcctgtttgc ctttgcagtt gcggcacaga tgctgacatg
     1681 gtgtgaaacg cgcagtgggt ggcgttcggt gggggccata ttgctgctgg ctgttattcc
     1741 ggcgcttaac cctgccacca gtgatgctgc ggcggtggcc ggccttgtga tgacggtgct
     1801 gaccgctggt ctggtgtcct gtgcaggaaa atcattaccc cgtttctggc ccggggattt
     1861 tttcccggct ttctatgccg gtcatctggc tgtgctgggc gttctggtac tgtgacgggt
     1921 gtggtatctt tggccgcaag aggatgattc ggcagaggca gaacacagca tgacagagca
     1981 gaagcgaccg gtactgacac tgaagcggaa aacggaagga gatacgcctg tccgcagccg
     2041 gaaaaccatc atcaatgtca ccacgccacc aaagtggaag gtgaaaaagc agaagctggc
     2101 cgagaaagca gcccgggaag cagagctggc ggcaaaaaaa gcgcaggcca gacaggcgct
     2161 gtccatttat ctgaacctgc ccacactgga tgaggccgtg aacaccctga agccctggtg
     2221 gccgggattg tttgatggtg acaccccccg gcttctggcc tgtggtatcc gggacgtgtt
     2281 actggaagac gtggcgcagc ggaatatccc gctctcgcat aaaaaactgc gcagggcgct
     2341 gaaggccatc acccgttcag aaagctatct gtgtgccatg aaagccggtg cctgccggta
     2401 tgacacggaa ggatatgtga cggagcatat ttctcaggag gaggaagcgt atgcggcagc
     2461 gcgtctggat aaaatccgcc gccagaaccg gataaaggca gaacttcagg ccgtgcttga
     2521 tgagaaataa aaaaagcctc cccgaagaac tcaggaggca gaaacaataa ggcttaaaag
     2581 gaaataactt ccatgcctga aggaaatcgg tccactgaat tatttaacaa cataatgctg
     2641 acgggttaaa gggggagata aaaatgaatg gattcagaaa cagttcacgg aacggtcagg
     2701 tctggcgctg gcagcttgcc ggaaagcggg ctgtaacact ggaagtcaga gggctctgga
     2761 tggaagcggc tgaagcatgg caacgggcgg cttatgttgc gccccggaca gactggcagc
     2821 agtttgcccg aaaaagggct gaacactgcc accggcgctg ccggggaagg gtgtaacgaa
     2881 aaaaacagcc ttcattacac cagaagtcag aaggttgaaa ggagtttat
//
LOCUS       DAHBHC010000219         2922 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHBHC010000219 DAHBHC010000000
VERSION     DAHBHC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.224"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="M2A35_005241"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="M2A35_005241"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCB7201385.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIQFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="M2A35_005242"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="M2A35_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCB7201386.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASENLSKEDAHALG
                     IFLRDLTALDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..>2922)
                     /gene="narH"
                     /locus_tag="M2A35_005243"
     CDS             complement(1565..>2922)
                     /gene="narH"
                     /locus_tag="M2A35_005243"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCB7201387.1"
                     /translation="YKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYEPFD
                     FDYQNLHTAPEGSKAQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKNFDN
                     IQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCRGWR
                     MCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADA
                     IERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMAMEW
                     KLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLANLLT
                     AGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIANYE
                     DRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKTEPH
                     P"
BASE COUNT          674 a          861 c          743 g          644 t
ORIGIN      
        1 cttccttaaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaactgaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctcca gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatgg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacag cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgagccaggt actccagata cagcggcaga tggtccggca attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccaacagg tcaaccatcg cctggccgcg gtcgcgggat
     1321 tcgccgtgca catgttcaaa cagcaacagc gacgtggcgc gaccacggtc gaacagttcg
     1381 ctgtactgcg cctgggcatc cagcggatcc agcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacaaattt tccgacgcgg caatcgcctc aaacatctcc
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcagtca
     1801 ggccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttgtcaaa ctcaccgccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgctttg ctgccttccg
     2761 gcgcggtatg caggttctga tagtcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcgaag attttaccca gcagcatggc gcggttaccc atgcgcggct
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tt
//
LOCUS       DAHBHC010000220         2919 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHBHC010000220 DAHBHC010000000
VERSION     DAHBHC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.225"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(43..441)
                     /gene="traA"
                     /locus_tag="M2A35_005244"
     CDS             complement(43..441)
                     /gene="traA"
                     /locus_tag="M2A35_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCB7201388.1"
                     /translation="MDAVLSVQGASAPVRNKSFFPKFTRLKKYFSFRPSRLNMLRLAR
                     AVIPAAVLMMFFPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAV
                     MYMMTKNVKFLAGFAIISVFIAVGMAVVGL"
     gene            complement(475..714)
                     /gene="traY"
                     /locus_tag="M2A35_005245"
     CDS             complement(475..714)
                     /gene="traY"
                     /locus_tag="M2A35_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCB7201389.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVTEESESTFKEL"
     gene            complement(822..1469)
                     /locus_tag="M2A35_005246"
     CDS             complement(822..1469)
                     /locus_tag="M2A35_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HCB7201390.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LQLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWNVVVE
                     STEVNSLNLFVWRFFERYVVSSFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(1661..2044)
                     /locus_tag="M2A35_005247"
     CDS             complement(1661..2044)
                     /locus_tag="M2A35_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCB7201391.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKASAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
     gene            2320..>2919
                     /locus_tag="M2A35_005248"
     CDS             2320..>2919
                     /locus_tag="M2A35_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCB7201392.1"
                     /translation="MILKINQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAV
                     GAYNAGFRKSERQNQRRLAYASEVYRIYTGIKSSKGIRLP"
BASE COUNT          924 a          649 c          520 g          826 t
ORIGIN      
        1 tcttaagttt attctcgtct cccgacatcg ttttatttcc tgtcagaggc caacgacggc
       61 catacccaca gcaataaata cagagatgat ggcaaaaccg gccaggaact tgacgttttt
      121 ggtcatcatg tacatgaccg caccgaccag aacttcagcc agaacaaccc atttaacaac
      181 actggagtcc ttaccgaagg tcgccttaac cgtggtgtta ccacttgcca tcaggtcctg
      241 accactgctg ccagcggcca tcgccagctg cgggaagaac atcatcagaa cagcagccgg
      301 gatcacagcg cgagccaggc gaagcatatt cagacgagag ggtctgaaag agaaatactt
      361 tttcagacgg gtaaatttag gaaaaaacga cttatttctg acgggcgcag aagcaccctg
      421 aacacttaaa acggcgtcca tatgattcct ctgtttaata ttgatagata aaaactaaag
      481 ctctttaaat gttgactcgc tctcttcggt cacctccctg aatatttctt cgttataaaa
      541 atctggataa cgttgcaaat gatcctgcaa cctcaccaat gcttcgtttg ttttcgttct
      601 tccactacgc tctctggctc caagtaataa tttattcgtt tcctcatcaa gggtaagtaa
      661 aacctttcta cttggcgcag atcttaaagc tcctttaatc accctccctc ccataaataa
      721 aagagattta gtcaaaatat tcacataaga cgcatacaaa aataaacaac aaaatagtaa
      781 attacgttaa caaaaaccgg cacttttagt gccggaaatg cttacatttc tttacagtga
      841 agactgttaa tattactagc gaagtattga tatagataaa ataacacatc agagattata
      901 gcatatcgca accactcatc cctgtctgag aaaccatagt gttttttaaa attcataaat
      961 atttttttag attggtcaac agagatatcc attctctgag aagaatactc atgactataa
     1021 cccaaaagat acggtattat cttattaaag cgatatttgt caggttcaca actaaactcc
     1081 ttgcccttta taaaaaagct actaactacg tatctttcga agaatctcca gacaaacaaa
     1141 ttcaagctat tcacttccgt actttcaacc accacattcc atgatacatc attcaatatt
     1201 acaccattaa taatacttac tccattcgag gacgagaaag catcaacttc agcagtatga
     1261 aataaaattt gcgtatcaat atcaatacca gaaaaccata tttcagaatc actattctga
     1321 cactttatta attgtaaaaa aacagagttt gagtctatga attttcctga ctctgtgcgg
     1381 acacagactg gatattttgc ggataaaata atttccggca ggcctgattg acgtatgagt
     1441 gctctttctc tagtatccat cgcacacata ggaacctcct caaaggattc tatggacagt
     1501 cgatgcaggg agggacaagc tccctgcatc ggcgattttc aagttcaaat tttaacgtgg
     1561 cattaaccac gtaattaata tactaattat tctgattatt gtcaccagtt tgaaccatta
     1621 aaaattctga tatttgaaca aagtcaaaat ttatttccct ttattcatca tcattttttg
     1681 gaaagaacct ttccatctca accgacacct tctctctgat atcttcaacc atattggcat
     1741 attcaaactt tggatttcct gagacatgag gactgagaga ctcaataccc aaaattttcg
     1801 ctactgatga ctgtgttttt acaacgcatt caagaagtag tttattaaac tcggcctgat
     1861 taaatgcaga cgctttgcgc tccatctgag cctcatatac acgaagaccc agttcaagaa
     1921 gcattgaagc cgttgctgaa aaactgacat ctttttctct cgccccctcc tgtcgacgtt
     1981 tttcaataat cgcgtttatt ttttcataag catcattgct gatatacaga ttcaccttag
     2041 ccattagata ttaccttttt taaacataaa acaaagttaa tttcgaaaca ctagaattca
     2101 tagtacaaac aggggcgcac cgctagcagc gcccctgacg gtatcctata aaaaaacaca
     2161 ccgcgccgct agcagcaccc ctaatataaa ataatgtttt ttataaaaat agtcagtacc
     2221 acccctacaa agcggtgtcg gcgcgttgct gtagctgcgt taacgacgct gctttaaata
     2281 aatcagattt aaacaatata aatccacaaa tacaactcaa tgatattaaa gataaatcag
     2341 caaaagcttg tttttgcgtg gggtgtggtg cttttggtgg tgagaaccac caacctgttg
     2401 agcctttttg tggagtgggt taaattattt acggataaag tcaccagagg tggaaaaatg
     2461 aaaaaatgga tgttagccat ctgcttgatg tttataaatg agatctgcca tgccactgat
     2521 tgctttgatc ttgcaggccg ggattacaaa atagatcctg atttactgag agcaatatca
     2581 tggaaagaat cccgttaccg ggttaatgcc atcggtatta atccggtaac gggatatggc
     2641 agcggactga tgcaggtaga ttcccagcat tttaacgaac tggcccgcta cggaattaag
     2701 ccagaacatc tgacaacaga tccctgcatg aacatttata ccggtgctta ttatctggca
     2761 atagcattta aaaaatgggg cgtcacctgg gaggccgttg gtgcatacaa tgcaggattc
     2821 aggaaatccg aacgccagaa ccagagacgt cttgcctacg catcagaggt ttaccggatt
     2881 tataccggga taaaaagcag taaaggtatc cggcttccg
//
LOCUS       DAHBHC010000221         2886 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHBHC010000221 DAHBHC010000000
VERSION     DAHBHC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.226"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(180..776)
                     /locus_tag="M2A35_005249"
     CDS             complement(180..776)
                     /locus_tag="M2A35_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201393.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(773..1492)
                     /locus_tag="M2A35_005250"
     CDS             complement(773..1492)
                     /locus_tag="M2A35_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCB7201394.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(1489..1923)
                     /gene="psiB"
                     /locus_tag="M2A35_005251"
     CDS             complement(1489..1923)
                     /gene="psiB"
                     /locus_tag="M2A35_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCB7201395.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(1978..>2886)
                     /locus_tag="M2A35_005252"
     CDS             complement(1978..>2886)
                     /locus_tag="M2A35_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201396.1"
                     /translation="CEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNVCV
                     QRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKS
                     LALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKSRLAA
                     LLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAIGF
                     HMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKDNR
                     WVPGWMCAPHPQTDATERTDNLADAA"
BASE COUNT          619 a          880 c          748 g          639 t
ORIGIN      
        1 ctgaggccac agagctgaaa acccacaagg ccatgccccg catggccggg taaaagcgga
       61 gccgccggcg acttccttat tccggcgagt gcagaggaca cgccgcatga cctccagacc
      121 acccctgata tgaaacgatc atgatcggcg tgtccctgcg taaaatatac atgtctggtt
      181 caccggagaa tatctgtgat atccacaagt tttctgtcgc acagccagtc ctgcgcttca
      241 cgcaccacgc tactgtcggg tttcaccgct ttcagcgatg atgccagtcg cttgatcaac
      301 ataaccagtt cccgtgtctg tatgtccggc attgtctccg gcggagcaga gggactctca
      361 tttccgacgc ccatcccatg cgcttccagt gtggcacgga caaaccaggc ggcatcgacc
      421 agttgctggc gaatccaggt cagatcggca tttctgtcac gggctgccat tcgctcaccc
      481 gacaacgcct tgctgatatg gctgcgcacc agattatcca ggagttcctg cgcttcttcc
      541 ggtgaaaagc cacctggtat ctctgtgcgc aacagtcctt tcctgtacag tagctgcagc
      601 agatcaatct gcgaccgcac tgcaactatc agtacataac ggacaatatt cgcactggcg
      661 gtggtttctg tatcattcag cagttcctcc ggatcattac caccggtatt gcagagcaac
      721 ggcaacagag gtcgcatcca cacgggaacc tgatattgtt cgtctgattt catcatattg
      781 cgttctccag cttgttcggg atcatccact gctcaagtgc acgtacctgt accggtgcac
      841 cacgaccggc ctcaccggct tcaaaaatca gccgccagag tggttcctcc tgccagaaaa
      901 accggtcaaa tgatgacagt gatggaaaac gcccgcacca cccccagaaa atcgtttcca
      961 gatcatgctc ctcaacaaca ccagaccagc gggacaacca gcttccgacc gtggcgggag
     1021 tatgaaacgc aaggtcaatc tgcgcctgag caaaacatgt ctggtattcc agttcccgtc
     1081 gttctgcctc acgttcccgc tggcggctgt atttctgctc tgtcagcgtg gttttctgac
     1141 gacgccgctc accttcacga aaaggaacgg acgggaacag acgatgcccc gcatcggatg
     1201 gcaggggaag aacacagact tcaccaaaac tttcaatcag tttatccacg gcagccagcc
     1261 acaggagacg gttgccatga aattcctcct tcgtcagcgc aggagcaaaa aagcggagat
     1321 cggtcagcga aatccggcga ctgccattca ggcagcgaaa gaaagcgctg gcataaggcc
     1381 atgctgaaag tgtgcgtccc tgacgacgac gttgttcggt gacagccaca gcctgcatgg
     1441 cggcctgttg ctctgcgctg agggggatca gtgcccgtga acgtgcgctc atgcctgacc
     1501 tccttctgcc atcaggatat ggatgatgtc gttaacactg tacccctgcg catcaagtgt
     1561 cgcggcaagc gccagcgtgt gactgatagc gacaggcgtg aagatatgtt gcacctgaac
     1621 gacggcgaaa ggctgtccac cacaattcac cagaaccagc atccagagct gagagacgtc
     1681 tcccggcgaa cataatgcca ggtggaaacg ttcgtgggct ggcgtgaaac gaacctggac
     1741 ggggaaaaag cccccgaact cactgccata ctcgccattc atcatccagt tagccggtgc
     1801 gtccacttca cgcatcacct cgtgagtgag attacggcgc atatcgcttc ccgcagcacg
     1861 gatatcttca tattcctgtg cgttcatgga ctgcagggca ttcagggtca gttcagtttt
     1921 catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg ttgttcatca
     1981 ggcggcatca gccaggttat cggtgcgttc agtggcatct gtctgtggat gtggtgcaca
     2041 catccagcct ggaacccagc ggttgtcttt catatggtgc tctgcatgtt cagccgcatc
     2101 acctttcttc atcttctccg cgtcccgtgc ggcaccggac agtccggcct cattcagggc
     2161 tgcgataatc tgcggctttt tcaggtgtcc gaagaagttt gcttttgtcg gctgccacca
     2221 gtcgcgcatg tggaatccga tagcgctttc cagcgagtca agcggactgc gtgacgtgtg
     2281 accacactca cgggtctgga caccgttaag gctgcatgcg gtgcagaaac tgagcaggga
     2341 taacagcacc tcctgactga gggacaggaa tgtcgtcatg tcccgtgacc atccctccgg
     2401 cagcagggct gcaagacggg atttttctgc catcagcgcc atgaatgcgg caccttcctt
     2461 ccccgatggc gcatcgctgg tcagcgaata atgttcacat tccaggctga tttgtgctgg
     2521 tttactgtac gccccgctgc caaacacatt caggcagagc gtccatgcca gcagtgccag
     2581 agatttgtcc ggctgctgca tgagtgccgc ctggactgcc agcgtgcgct ctgaggacat
     2641 tttcgtcagc aacggcaggc tgatttcctc cactgacgct ttctcctgca cctgttccgt
     2701 gcggtcagcg tcgtcagtcg cgtcatcttc actgcgcaac tgcacaccac gctggacaca
     2761 tacgttgcca taacgccagg acaccaccac accgcttccg gcacgtatct ccggcgtcca
     2821 cgctctgacc ttcgccatgc agcgcatcag cttcatttct gcttccagca ggtcggattc
     2881 ctcaca
//
LOCUS       DAHBHC010000222         2806 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHBHC010000222 DAHBHC010000000
VERSION     DAHBHC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.227"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..195
                     /locus_tag="M2A35_005253"
     CDS             <1..195
                     /locus_tag="M2A35_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201397.1"
                     /translation="RQMTEGELKRLAERQLTKWAKHVSNGLSVPPVRRQLAAPKRPAG
                     PTPIELLKQEYERRKAAGFV"
     gene            228..974
                     /locus_tag="M2A35_005254"
     CDS             228..974
                     /locus_tag="M2A35_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCB7201398.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELRKAGFVD
                     KSVYTWRVAYNNVQQEQPAPAELSEETTTATVAKISECDLTATVEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDKLPAEPKSVPVTENDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWERVCAALRELNKHRDMVAGICRKSGQ"
     gene            971..1135
                     /locus_tag="M2A35_005255"
     CDS             971..1135
                     /locus_tag="M2A35_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201399.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKARVKGISLILR
                     GNFHQSTKYP"
     gene            1834..2592
                     /gene="paa"
                     /locus_tag="M2A35_005256"
     CDS             1834..2592
                     /gene="paa"
                     /locus_tag="M2A35_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HCB7201400.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPEADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
BASE COUNT          815 a          575 c          740 g          676 t
ORIGIN      
        1 cgccagatga ccgaagggga gttaaaacgg cttgcagaac ggcagctgac gaaatgggca
       61 aagcatgtta gcaacggcct gagtgttccg cctgtccggc gacaactggc agcacccaaa
      121 cgtcctgcgg gaccaacgcc aattgagttg ctgaaacagg aatatgaacg ccggaaagcg
      181 gctgggtttg tctgatttga gaagtaattt ttaccgggag gaaatttatg gagactgttt
      241 ttgacgcact gaaagcgatg ggaaaagcca cgtcggtaga gctggctgcg cgacttgata
      301 tcagtcgtga agaagtgctg aacgagctgt gggaactgag aaaggctggc ttcgttgata
      361 aaagcgtata cacctggcgt gtggcttata acaacgttca gcaggaacag ccagcgccgg
      421 cagaactgtc ggaagaaacc accacggcaa cagtcgctaa aatttcggag tgcgatttaa
      481 ctgcgacggt tgaacaacgc ggaccacaaa cggctgatga gctggctacg ttgttcggta
      541 caacatcacg caaagtggct tcaacgctgg caatggcaat cagcaaaggt cgtctgattc
      601 gtgtaaacca gaacggtaaa tttcgttact gcatgccggg cgataagtta ccagcagagc
      661 cgaaatccgt gccggtaacg gaaaatgatg gtaaagcctt tcctcagcca gcaggtgttg
      721 cgttaccagt acaggaagct gcaacacagg aagatattaa aacagaaact gtggctgata
      781 ttgtgcaatc gttgccatcg tttactgaaa cgcgagcgga tgacctggtt ttaccatcgc
      841 tgcatatggc aaaccgcgaa ttgcgtcggg cgaaaagtca tgtccagaag tgggagcgag
      901 tctgcgccgc gctgcgggag ctgaacaagc accgagatat ggttgccggg atttgtcgga
      961 agtccgggca atgagcggat ggtgcaggcc tgaaattatg atactaacaa tgaaggtaaa
     1021 atgcatcggc agtctgattg gtcgtagtga ggcggcggtc aggatgaaag cccgggttaa
     1081 gggaataagc ctgattctgc ggggtaattt tcaccagtca acaaaatatc cgtagcgcga
     1141 taacggtcaa aaatcatggc gctgacactt ttgtgccact gtagatgact gtacctaagt
     1201 tcaggggaga agaacacgtc cggtgggatg gtcgggccag atttaaaggg caggtcatgg
     1261 ctccagccag tacgctggca atggaggctg cctggctgga aattgatatg ggaaccacgc
     1321 cactcaggga tttactgccg gtccagagaa taaattcctg ttacggttac accactgtga
     1381 tcttgcaagt gcaggaaagt aggtctacac ggtaacgcga gtgcgtgtaa cttttgatgt
     1441 tatttccgta gaaacaccgg acaaattttc gctggcaggt catgctgaag gtataaatct
     1501 gcagattatg gacaattacg gatatccggc aagagccgga aaaagcatgc cgcctctaat
     1561 tctcagtgga agatggactt gattatactc attgcattgt cagaaatagt tatccattaa
     1621 aggctggcta ttccaaacag gatgttgatt acaaaaatgt aatcaacatg taaggtttat
     1681 actcttcaat atgcgtataa ttttccttat tttgttaact ttaaataaca agctatgcac
     1741 gaggtaaagt cggataagtt tatctggatg taatatatat tatttgtagt gtttataact
     1801 ttatttcatg ataaccaata aaaggagttt tttatgagga acataatggc aggtttttta
     1861 atattcctgt cttctgctgc ttatgctgat atcaatctgt atggtcctgg tggcccgcat
     1921 acagccttgc ttgatgcagc caaactttat gccgaaaaaa caggtattat agtgaacgtt
     1981 cattacggcc cacagaacaa atggaatgaa gatgccaaaa aaaatgcaga tatcttgttt
     2041 ggcgcatcag aacaatctgc tctggctatc attcgggacc ataaagacag cttcagtgaa
     2101 aaagatattc agcctcttta tctgcgaaaa agtattttac tggtaaagaa aggtaatcct
     2161 aaaaatatcc ggagtattga cgacctgacc agacccggga ttggcgtaat tgttaatgat
     2221 ggtggtggta ccagtaatac atcaggcact ggcgtctggg aagatattgc cggacgtaaa
     2281 gggaatatag aaactgtcgc cgcaatccga aaaaatatta ttttatatgc gcccaatagc
     2341 ggaactgcac gtaaggctct tgagaatcag cctgaagcag atgtctggat aacctgggct
     2401 gactgggcag ccagtaatcc agaaattggt gatgtcgtgg aaatagcgcc agactacgtg
     2461 atatggcgtg atatgaacat tacagtacgt caggatgcaa atgatgaaac ccgtcgattt
     2521 gcagaatggc tacaaaccga tgaagcggcg cctgcattca aaaaatatgg ctggaccagg
     2581 aaaggcactt gacatcctcc acgtccttca gaacgtggat tctttttccg gatgccgcgc
     2641 cagcggcatg taggggcagc tcacaaaacg gaaaaaattg tacgctaagc ctcgccaggt
     2701 gaactgaatt cattccgata tgggaattcc catatcggac gaaaacggct tgctgtaacg
     2761 gcagagttaa gtagaattgc tgcgggtgct tgaggctatc tgcctc
//
LOCUS       DAHBHC010000223         2794 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHBHC010000223 DAHBHC010000000
VERSION     DAHBHC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.228"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1074..1934
                     /locus_tag="M2A35_005257"
     CDS             1074..1934
                     /locus_tag="M2A35_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7201401.1"
                     /translation="MKITDHKLPEGIALTLRMPEGNQKPPLIILCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVISWAEQQTDIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCLVSQLAFADGDTLVTGGMSEPERTSFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKEFFGKVKTRHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGRDSVNPPEQGKALYDAVASADKTLYEEAAARHYDLYEGEFFERVVA
                     VQTQWFKKHL"
     gene            complement(2459..2653)
                     /locus_tag="M2A35_005258"
     CDS             complement(2459..2653)
                     /locus_tag="M2A35_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201402.1"
                     /translation="MWFFSTVLCLLVIICCVAGIVNAMAVRREFKRSREQEYHARIRE
                     ATGERILRERYEHFRRRRER"
BASE COUNT          770 a          665 c          655 g          704 t
ORIGIN      
        1 tttacccttc tctgtgtgtc aacgccacga tgtttgaccg tgacttatat cagtacgctg
       61 acaacgggaa tatcagccac agacgccccg tcactgctca ggagatgacg ggtacctgag
      121 gagcgaagcc atataaaaaa gccagccatc caggctggcg agcatgtcat gttccttcgg
      181 aaaataaaag cacgttatcg gacatcagtg ctcacataca taatattata tgccatatgt
      241 cattccggat attactgtct gtctttttca ggaatcagtt attatccgtg tgccgtggtt
      301 acctgacaac atcataaagt gaaccagtac ggatatacag gcagcggtcc ggtaacacag
      361 atgtgtataa tatccacacg ctgatacggg ttcagcgctc aaaaaaaagc ctgcacaacg
      421 cctgacccgt tataaagcag agacaccccg cgatatcttt aagatccccg cgccataatc
      481 ttacaggaga atgcgttcat ctacgtcagt aaaaaatgac tctgcacgca ctgaaataca
      541 gaagactgag ataaacactc agggaattta aatatattca tataacaaaa cgacatatat
      601 tcattcccga cctgtcataa aaacaccccg gccggaaaag ataccatccc gatgaacagc
      661 gctgagcaga cagcatattc accgtaacac cgtctgacga aaaatatcac tgttgtcgcg
      721 ccactcatcg gatttcatgc aggttacagc catgttaaat tgaaatttaa tttcctgagg
      781 caatgcagga agtattaaaa attattcaca gcgcaaatgt aagtactccg cttaaaggta
      841 tttttgtatc gtgctgtctt tatcgccgga tttgtccgta ctgttccggt ataagccccc
      901 gtctcatgta catcgtccgg gggctttcaa ccgtactgat atcatgactc cgcttctgcc
      961 ggggacttct tctgccattc ccctcctgcc cactggcgtt agcgggcgcc cggcgctgag
     1021 acaggtatga cgctgattct gattattaat aaacgacgta acacaggata tttatgaaaa
     1081 ttacggatca caaactcccg gaaggtattg ccctgacact ccggatgccc gaaggaaatc
     1141 aaaaaccgcc tttgattatc ttatgtcacg gattttgcgg tatccggaac gttctgctcc
     1201 cctcctttgc gaatgcgttt acagaggccg gattcgcaac gattactttt gattatcgtg
     1261 gattcggtga gagtgaaggt gagcggggcc gcctggttcc ggcaatgcag accgaagata
     1321 ttatttcagt tatcagctgg gcagaacaac agacagatat tgataatcag cgtatcggac
     1381 tgtggggcac atcgctgggt ggctgtcatg ttttcagtgc agcagcgcag gatcaacggg
     1441 tgaaatgtct ggtcagccag ctggcctttg ctgacggtga tactctggtt accggaggga
     1501 tgagtgagcc ggagagaaca tcatttctgt ccacactgaa taaaatggca gagaagaaga
     1561 aaaacaccgg taaagagatg tttgtggggg ttacccgggt actgagtgat aatgaatcga
     1621 aggaattctt cgggaaagtc aaaacccggc atcccgaaat ggatataaaa atacccttcc
     1681 ttaccgtaat ggaaacattg caatataaac cggcggagag tgcagccaga gtacagtgtc
     1741 ctgtgctcgt tgttattgcg ggcagggata gcgttaatcc tccggagcag gggaaagcat
     1801 tatatgatgc tgttgcttct gctgataaaa cattatacga ggaagcggca gcccggcatt
     1861 atgatcttta tgagggtgag ttttttgaac gtgtcgttgc agtacagacg caatggttta
     1921 aaaagcatct ttaacggtca gtgtgaatac ggataaaggc ccgttgaagt aagcttaaga
     1981 tgattatgcg ttgcttcagc ggccatattt ccataaaggt cagcgcaatg atggcgggga
     2041 tgcggaccat taccccggcg aatctcagca tctttcagtc agggatgttt tccgcacggc
     2101 agatgcgcag caacggccgt gatttgtcat accatcagtg atgggacaga cgccattcct
     2161 gaatatcagc ctgtattccc tttcgtgcgt gcctcctgaa tgtccttgta cggtgaaaat
     2221 gttcaggagg ctccggcacc ggaactgcgc gcgaaggccg gtttgtttaa acctgcaggt
     2281 tacaaacagg tgatggtcgt gcaacagtca ctcatcggat ttcatgaacg ttatcattat
     2341 attaaattaa tgttgtaccc tgtgtggtac gcaagaagca cgtagagcag aaactataaa
     2401 aacattaccc ttgatcaaaa cgtaagtgat tccccctttc ctgaaggggg ttttttattc
     2461 aacgttccct tcgccgccgg aagtgttcat accgctcccg cagaatacgt tcacccgtgg
     2521 cctcccgtat ccgcgcatgg tactcctgct ccctgctcct tttgaactcg cgccgtactg
     2581 ccattgcatt cacaataccg gccacacagc agataatgac aagcagacac agcactgttg
     2641 aaaaaaacca catactgtca gcgccagtga tataagacgg taattcacca tttggattgt
     2701 ccgctccacc caacatgttg tttccttaag gttctcacac cagaaaggac atcaacatgc
     2761 tgagcagaga ggacttttac atgataaagc aaat
//
LOCUS       DAHBHC010000224         2714 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHBHC010000224 DAHBHC010000000
VERSION     DAHBHC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.229"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            89..508
                     /locus_tag="M2A35_005259"
     CDS             89..508
                     /locus_tag="M2A35_005259"
                     /inference="COORDINATES: protein motif:HMM:NF017542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HCB7201403.1"
                     /translation="MANTTRKKVKIVGKKQYLDPTTGEVSDFQVINIEERDANFHKIW
                     LSNVIQSLDLIGNQKIRFAFWLMDQANKDNEITLTLRQMSELSKISLETVRLTVKSLI
                     ESDFLRRKNMGVYQINPNSIFKGGNGNRLNVLFSYQK"
     gene            complement(784..1791)
                     /locus_tag="M2A35_005260"
     CDS             complement(784..1791)
                     /locus_tag="M2A35_005260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201404.1"
                     /translation="MLLPPLNTPFCDWITVTYRSERPDLSYITRLFFEGLHASVHPLG
                     GVDSGWSAIGNPRMALTGAGSELTGIPITQFVILARFADKSYHLVFQISGLGLQLLRL
                     EPELFRELMRELGSCPHRVTRLDVGMDLPVDGADYLADFKSRFPSGYVQFTRKRAALS
                     WVLSVRDDGRDTGTVYAAKGTRSRVKMRIYDKAYEVLQRRKLTIPPRTRVEFALETEF
                     GASLYDALRPESLFWHLASPDILGKKPADVPAWFKRESFTPAPRERRYKSDKPPLHVM
                     KSYVDDTQLLDGLIEYVDRNIGSDGRMLLKNHIGRYIDQYDESRKILLSDKASTTDKA
                     S"
     gene            complement(2054..2200)
                     /locus_tag="M2A35_005261"
     CDS             complement(2054..2200)
                     /locus_tag="M2A35_005261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201405.1"
                     /translation="MTVHDRSIPEDVYQSFCQLRDSVDGFSAVARLIPDGESDGFRLS
                     SWLS"
BASE COUNT          860 a          621 c          629 g          604 t
ORIGIN      
        1 aaaaaaaaat agtacaaaaa gcattgacta ggacaagaga cctatttaca ttaggacttg
       61 tggtaggtat ttaatcaagt ggggcaatat ggccaataca acgcgtaaga aagtaaaaat
      121 agtaggaaaa aagcaatatc tcgatcctac aactggggaa gtctctgatt ttcaagtgat
      181 aaatattgaa gagagggacg ctaatttcca taagatatgg ctgtcaaatg taattcaaag
      241 tctagattta ataggaaacc aaaaaattag gttcgcattt tggttaatgg accaagcaaa
      301 caaggataac gaaataacat taacgttgcg tcagatgtct gagttatcaa agataagttt
      361 agagacggtc agactaacag tgaagtcatt aatagaaagt gattttttac gtagaaaaaa
      421 catgggcgtt tatcagataa acccaaactc tatatttaaa ggaggtaacg gtaatcgact
      481 taatgtactt ttttcatatc aaaaatagag caaaaacgag agtgcgtggc gtgaattctt
      541 caggcccgga cacgccaccc ccgccccacg cgacagcgtg tgacgtgtgt gtcgcgaccg
      601 ggcgagggtg aatatagcct gagccttccc cgtgtattct ttgtacgaca cagttaatat
      661 atttaaagat attagtcacg ttttagaaag caggcttaat aagcaacgca aaaagaaaat
      721 atataaacag aagttatttc ccgcgtggcg aatgtaatta atacagagca cgcaggaaat
      781 aaattaactt gctttgtcag tggttgatgc tttatcagaa agcaatattt tccgactctc
      841 atcgtactga tcgatataac ggccaatatg atttttcagc aacattcgac cgtcagaacc
      901 gatatttcga tccacgtact ctattaaccc atcaagcagc tgcgtgtcgt caacatacga
      961 tttcataacg tgaagtggtg gtttgtcgct cttatatcga cgttcgcgag gcgcaggtgt
     1021 aaaggattcg cgtttaaacc aggctggaac atcagccggt ttcttaccca gaatatcagg
     1081 cgatgcaaga tgccagaaca aagactcagg acggagggcg tcatagagcg atgcaccaaa
     1141 ttccgtctcg agcgcaaact caactcgcgt acgcggcgga atggtaagtt ttcgccgctg
     1201 taacacctcg taagccttgt catagatacg cattttcaca cgagagcgcg tacctttagc
     1261 cgcgtatacc gtacccgtat cacgaccatc atcacggacc gacaaaaccc agctaagggc
     1321 ggctctctta cgcgtaaact gaacataacc agaagggaaa cgacttttga aatcagcaag
     1381 ataatcagcc ccgtccacgg gcaaatccat gccaacgtcg agacgcgtaa cacggtgagg
     1441 acaagaaccc aactcacgca ttaattcccg gaacagttca ggctctaagc gtagcaactg
     1501 aagaccaagc ccggagatct gaaaaacgag gtgataactc ttgtcagcga accgcgcgag
     1561 gataacgaac tgagtgatcg gaatacctgt aagttcagaa cccgcacctg taagagccat
     1621 gcggggattg cctatagcag accaaccaga atcaacacca ccaagcggat ggaccgaggc
     1681 atgtaaacct tcaaagaaca aacgggtgat gtacgacagg tcaggacgct cagaacgata
     1741 cgtgacagtg atccagtcac agaaaggagt gttgagagga ggaagcagca taagcagagt
     1801 gccaaagcga ctgtgaattg ttttttccaa atttctatgg ggggtgttac taaaaccccc
     1861 cgacagagca acattcaggt acagaaaaac ccgcttagca gggcgatggc ccactgcgtg
     1921 aaccatcacc ctcatcaagg gtatctttcc tgagtgtcgt caatcactaa atcggaaccc
     1981 actggcaggc cagcggggcc ccacgattta gcgtttgacg aattcagcat cagcgataga
     2041 acgccagtga acatcaagac agccatgaag agagtcggaa accatccgac tcaccatcag
     2101 ggattagacg ggcgacggcg gaaaaaccat caacagaatc gcgtagctgg caaaaagatt
     2161 gataaacatc ttcagggatg gaacgatcgt gaacagtcat ataggcctcc attcaagccg
     2221 taaggtttat gtgattagaa aagcccctta gagccacctg aggggctttt cgctattaga
     2281 tcacccaaac ccacaccttt caacgttttg ctcgtcagag agcgttttag accccttatt
     2341 ttcgattttt ttactacgtt gttgtcgtca gtcgtcacgg caggcgctcc cgtcctcgtc
     2401 cctgcgggcg ggtgcggctg gcgttcctcc ttcctccaac cactcaagac aaaaaccgat
     2461 aaccggagat caaaacgacc gagagagaag aaaccgctcg ccgcagaccc agcgaccgaa
     2521 cgtagtgagc gagtggtcga ggaagcggaa gagaaccgaa cagacatgaa gcacttacgc
     2581 gctgttataa aacatagata agagtgagca aaagcctgaa tcatagataa ctcattgttg
     2641 cctataactt ttgcaggaaa aatcctgtac tagatttcta ggtgtcttgt actagatttc
     2701 tggcacaaga cacc
//
LOCUS       DAHBHC010000225         2682 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHBHC010000225 DAHBHC010000000
VERSION     DAHBHC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.230"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>2682
                     /gene="rhsB"
                     /locus_tag="M2A35_005262"
     CDS             <1..>2682
                     /gene="rhsB"
                     /locus_tag="M2A35_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HCB7201406.1"
                     /translation="GVRIGAPTGVACSVCPGGVTSGHPVNPLPGAKVLPGETDIALPA
                     PLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHL
                     FPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWW
                     LLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAG
                     RHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDP
                     EYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQL
                     TSATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMT
                     WSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGH
                     ETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTS
                     ENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLT
                     HRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEA
                     LLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHR
                     ETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYS
                     TTGRLTSVHTTAANLDIRIPYATDPAGNRLPDPELHP"
BASE COUNT          581 a          843 c          838 g          420 t
ORIGIN      
        1 ccggggtgcg cattggtgcc cccaccggcg tggcctgttc ggtgtgcccc ggcggagtga
       61 cgtccggcca tccggtcaat cccctgcccg gtgcaaaggt ccttcccggt gaaaccgaca
      121 tcgccctgcc cgccccgctg ccgttcatcc tctcccgcac ctacagcagt taccggacaa
      181 aaacgcccgc gccggtgggg agcctcggcc ccggctggaa aatgcctgcg gatatccgct
      241 tacagctgcg cgataacaca ctgatactca gtgataacgg cggcagaagc ctgtattttg
      301 agcacctgtt tcccggtgag gacggttaca gccgcagcga gtcactgtgg ctggtgcgcg
      361 gcggcgtggc gaaactggat gaaggtcacc ggctggccgc actctggcag gcgctgccgg
      421 aagaactccg cttaagtccg catcgttatc tggcgacaaa cagtccgcag gggccgtggt
      481 ggctgctcgg ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct gcgccgctgc
      541 cgccgtaccg ggtactgacc gggctggtgg accgcttcgg gcgcacacag acgttccacc
      601 gcgaagccgc cggtgaattc agcggcgaaa tcaccggcgt gacggatggt gccgggcgtc
      661 acttccggct ggtactgacc acgcaggcgc agcgggcaga agaagcccgg cagcaggcca
      721 tttccggcgg gacggaaccg tccgcttttc ctgataccct gccgggttac accgaatatg
      781 gccgggacaa cggcatccgt ctgtctgccg tgtggctgac gcacgacccg gaatacccgg
      841 agaatttacc tgccgcgccg ctggtgcgct atggctggac gccacgcggc gaactggcgg
      901 tggtgtatga ccgtagtggc aaacaggtgc gcagctttac ttacgatgat aaataccggg
      961 gccggatggt ggcgcaccgt cacacgggcc ggccggaaat ccgttaccgt tacgacagcg
     1021 acgggcgggt gacagaacag cttaacccgg caggcttaag ctacacgtat cagtatgaga
     1081 aagaccacat caccatcacc gacagcctgg accgccgtga agtgctgcac acgcagggcg
     1141 aagccgggct gaagcgggtg gtgaaaaagg aacacgcgga cggcagcgtc acgcagagtc
     1201 agtttgatgc ggtgggcagg cttaaggcac agacggatgc cgcaggccgg acaacagagt
     1261 acagcccgga tgtggtgacg ggcctcatca cgcgcatcac cacgccggat ggcagggcat
     1321 cggcgtttta ctataaccac cacagccagt taacgtcagc caccgggcca gacgggctgg
     1381 aaatgcgccg caaatatgat gagtacggac gtctgattca ggaaaccgcc cctgacggcg
     1441 atatcacccg ctaccgttat gataatccac acagtgacct cccctgcgca acggacgatg
     1501 ccaccggcag ccggaaaacc atgacgtgga gccgttacgg tcagttgctg agcttcactg
     1561 actgttccgg ttatgtaacc cgctatgacc atgaccgttt tggtcaggtg acggcggtgc
     1621 accgcgagga agggctgagt cagtaccgcg catacgacag ccgtggacag ttaattgccg
     1681 tgaaagacac gcagggccat gaaacgcggt atgaatacaa cgccgccggt gacctgacca
     1741 ccgtcattgc cccggacggc agcagaaacg ggacacagta cgatgcgtgg ggaaaagcca
     1801 tctgtaccac gcagggcggt ctgacgcgca gtatggaata cgatgctgcc ggacgggtca
     1861 tccgcctgac cagtgaaaac ggcagccaca ccaccttccg ttacgatgta ctcgaccggc
     1921 tgatacagga aaccggcttt gacggccgca cacagcgtta tcaccacgac ctgaccggca
     1981 aacttatccg cagcgaggat gaggggctgg tcacccactg gcactatgac gaagcagacc
     2041 gcctcacgca ccgcaccgtg aagggtgaaa ccgcagagcg gtggcggtac gacgaacgcg
     2101 gctggctgac agacatcagc catatcagcg aagggcaccg ggtgacggtg cattacgggt
     2161 atgatgagaa aggccgcctg accggtgagc gtcagacggt gcatcacccg cagacggaag
     2221 cactgctctg gcagcatgag accagacatg cgtacaacgc gcaggggctg gcgaaccgct
     2281 gtataccgga cagcctgccc gccgtggaat ggctgaccta cggcagcggc tggctgtcag
     2341 gcatgaaact cggcgacaca ccgctggtgg agtacacccg cgaccgcctg caccgggaaa
     2401 cgctgcgcag cttcggccgt tatgaactca ccaccgctta cacccctgcc gggcagttac
     2461 agagccagca cctgaacagc ctgctgtctg accgcgatta cacctggaac gacaacggcg
     2521 aactcatccg catcagcagc ccgcgccaga cccggagtta cagctacagc accaccggca
     2581 ggctgaccag cgttcacacc accgcagcga atctggatat ccgcatcccg tatgccacag
     2641 acccggcagg taaccgcctg cccgacccgg agctgcaccc gg
//
LOCUS       DAHBHC010000226         2642 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHBHC010000226 DAHBHC010000000
VERSION     DAHBHC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.231"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            73..282
                     /gene="hha"
                     /locus_tag="M2A35_005263"
     CDS             73..282
                     /gene="hha"
                     /locus_tag="M2A35_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCB7201407.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            320..910
                     /locus_tag="M2A35_005264"
     CDS             320..910
                     /locus_tag="M2A35_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCB7201408.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKRLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RARQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGELLNKTEADQQSPEHRS"
     gene            1150..1410
                     /gene="repA"
                     /locus_tag="M2A35_005265"
     CDS             1150..1410
                     /gene="repA"
                     /locus_tag="M2A35_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCB7201409.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCESEGITQAQFVEKIIKDELQRLDILK"
     gene            1635..1709
                     /gene="tap"
                     /locus_tag="M2A35_005266"
     CDS             1635..1709
                     /gene="tap"
                     /locus_tag="M2A35_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCB7201410.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1702..2516
                     /gene="repA"
                     /locus_tag="M2A35_005267"
                     /pseudo
     CDS             1702..2516
                     /gene="repA"
                     /locus_tag="M2A35_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
BASE COUNT          726 a          615 c          687 g          614 t
ORIGIN      
        1 ccctggatat ggagacaccg taaataaagc gatggcaatc ttatcattta tctgttctca
       61 gcaggaataa aaatggcaaa aacaaaacag gaatggctgt atcagttacg gcgatgttca
      121 tcagtcaata cgctggaaaa aattattcat aaaaacagag acagtttatc aaacagtgaa
      181 cgtgaatcat ttaattcagc agcagaccac cggttagcgg aattaatcac cggaaagtta
      241 tatgacagga taccaaaaga gatatggaaa tatgtaaggt aataattcag cataattaat
      301 taccaggagc agataaacaa tgaagctgat tatttttatt cttattgttc tgattattgc
      361 agcgttactc atccggatta tcctcagaag tgtaaatcag cattctcctt tgctgatgca
      421 attgcatgcg gcaggtatac ggaccggtga tgcggagcga atactgtcca gcggtgaata
      481 ctggcaacgt cagaagacgc ttctgacaga aagggaagtc agttttatga aaagactgtt
      541 cagaattgtg gatatgaaac ggtggtatct gtgtccgcag gtacgggtcg cggatatcgt
      601 ccagctgaac gggaatatcc ggccacgagc acgccagtgg tggcagttat tcaggatggt
      661 gtctcagtgg catgttgatg tggttatcgt tgagcggcgt tcgttcagta ttgttgcagc
      721 agtagagctg gatgatgcca gccatttacg accggaacgc agacgccggg atattcttct
      781 ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga agccacgatg ccagaaaact
      841 gctgcagatg accggagaat tgctgaataa aacagaggct gatcagcagt ccccggaaca
      901 tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc ggaaacggga aaaagcaagt
      961 tttccccgct cccggcgttt ccataactga aaaccatact atttcacagt ttaaatcaca
     1021 ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca gatcttcgtc acaattctta
     1081 agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac gatccctgtt tgagtattga
     1141 ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct cattgagtca aaagcggttt
     1201 gtgcgcagag gtaagcctat gactgactct gagaaacaaa tggccgctgt tgcaagaaaa
     1261 cgtcttacac acaaagagat aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt
     1321 gagtactgcg agagcgaggg gataacacag gctcagttcg ttgagaaaat catcaaagat
     1381 gaactgcaga gactggatat actaaagtaa agactttact ttgtggcgta gcatgctaga
     1441 ttactgatcg tttaaggaat tttgtggcgg gccacgccgt aaggtggcag ggaactggtt
     1501 ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg ataatcttct ttaactttgg
     1561 cgagtgagaa agattaccgg ggccatctaa aaaccgtata gccaacaatt cagctatgcg
     1621 gggagtatag ttatatgccc ggaaaagttc aagacttctt tctgtgctca ctccttctgc
     1681 gcattgtaag tgcaggatgg tgtgactgat cttcaacaaa cgtattaccg ccaggtaaag
     1741 aacccgaatc cggtgttcac accccgtgaa ggtgcaggaa cgctgaagtt ctgcgaaaaa
     1801 ctgatggaaa aggcggtggg cttcacctcc cgttttgatt tcgccattca cgtggtctgc
     1861 gtcgacgggc tattgatgcg ctcctgcagg ggctgtgttt ccactatgac ccgctggcca
     1921 accgcgtcca gtgctccatc accgcactgg ccattgagtg cggactggcg acggagtctg
     1981 ctgccggaaa actctccatc acccgtgcca cccgggccct gacgttcctg tcagagctgg
     2041 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgaca
     2101 tcacgttcac accggcgctg tttgccgcgc tcgatgtgtc tgaggtggcg gtggcttccg
     2161 cacgccgcag ccgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2221 tgggtatgga tgaactgata gcgaaagcct ggcgttttgt gcgtgagcgt ttccgcagct
     2281 accagacaga gctaaagtcc cggggaataa agcgtgcccg tgcgcgtcgt gatgcgagca
     2341 gggaacgtca ggatatcgtc accctggtga aacggcagct gacgcgtgaa atctcggaag
     2401 ggcgcttcac tgccaatcgt gaggcggtaa aacgcgaagt ggagcgtcga gtgaaggagc
     2461 gcatgattct gtcacgtaac cgcaattaca gccggctgac cacagcttcc ccctgaacct
     2521 gacctcctct gaataatccg gcccgcgccg gaggcttccg cacgtctgaa gcccgacagc
     2581 gcacaaaaaa tcagcaccac atacaaaaaa caacctcatc atccagcttc tggtgcatcc
     2641 gg
//
LOCUS       DAHBHC010000227         2612 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHBHC010000227 DAHBHC010000000
VERSION     DAHBHC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.232"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..>2612
                     /locus_tag="M2A35_005268"
     rRNA            1..>2612
                     /locus_tag="M2A35_005268"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          692 a          572 c          818 g          530 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatgctgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgaca ctgtgtgttg ttgggtaggg gagcgttctg
     1201 taagcctgcg aaggtgtgct gtgaggcatg ctggaggtat cagaagtgcg aatgctgaca
     1261 taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt tcctgtccaa
     1321 cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt agtcgatggg
     1381 aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag aaggctatgt
     1441 tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg caaatccgga
     1501 aaatcaaggc tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa atgccctgct
     1561 tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc gacacaggtg
     1621 gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta ggcaaaatgg
     1681 tgccgtaact tcgggagaag gcacgctgat atgtaggtga agtccctcgc ggatggagct
     1741 gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag cactgtgcaa
     1801 acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt aattgatggg
     1861 gtcagcgcaa gcgaagctct tgatcgaagc cccggtaaac ggcggccgta actataacgg
     1921 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg gcgtaatgat
     1981 ggccaggctg tctccacccg agactcagtg aaattgaact cgctgtgaag atgcagtgta
     2041 cccgcggcaa gacggaaaga ccccgtgaac ctttactata gcttgacact gaacattgag
     2101 ccttgatgtg taggataggt gggaggcttt gaagtgtgga cgccagtctg catggagccg
     2161 gccttgaaat accacccttt aatgtttgat gttctaacgt tgacccgtga tccgggttgc
     2221 ggacagtgtc tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag
     2281 cacgaaggtt ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc
     2341 ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt
     2401 ctgaatggaa gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac
     2461 cgcccaagag ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct
     2521 ggggctgaag taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg
     2581 gtttagaacg tcgtgagaca gttcggtccc ta
//
LOCUS       DAHBHC010000228         2574 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHBHC010000228 DAHBHC010000000
VERSION     DAHBHC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.233"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..194
                     /locus_tag="M2A35_005269"
     CDS             <1..194
                     /locus_tag="M2A35_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201411.1"
                     /translation="FRKWLAEQGLIGQDVALSAIAAALHVHGVQRVDIIEPTQNMAIS
                     DIQAARCESFTISEGGRNE"
     gene            187..717
                     /locus_tag="M2A35_005270"
     CDS             187..717
                     /locus_tag="M2A35_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCB7201412.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPSD"
     gene            720..>2574
                     /locus_tag="M2A35_005271"
     CDS             720..>2574
                     /locus_tag="M2A35_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201413.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGNPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSDSETLAATPKAVKAAYDLANGKYTAQDATT
                     ARKGIVQLSSATNSTSETLAATPKAVKAVMDETNKKAPLNSPALTGTPTTPTAQQGTN
                     NTQIASTAFVMTAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNSLAGKQPKDAT
                     LTALAGLATAADRFPYFTGNDVASLATLTEVGRDILAKSTVAAVIEYLGLQETVNRAG
                     NAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSYMWFETGDNGNEY
                     FKWRSKQSTTTKDLMNLKWDALSVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEE
                     CLHIIPTRENEGENGDIGPLRPFTLNLRTGRISMGHGLDVTGDIFANRFAINSSTGMW
                     IHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGIYMINNSR"
BASE COUNT          695 a          633 c          687 g          559 t
ORIGIN      
        1 cattcaggaa gtggctggct gaacaggggc ttatcggtca ggacgtggcg ttgtccgcca
       61 ttgctgccgc acttcatgta cacggcgtgc aacgcgtgga cataatcgaa cccacacaga
      121 atatggccat cagcgacata caggcggcgc gctgtgagtc attcaccatc agcgaaggtg
      181 ggcgtaatga gtaattcact gttaccgcca tcagccagca gtttcatgcg ttgtgccgaa
      241 gctgtcggaa cgcgcattac agacatcccg gtagacctca acacactgtg gtcgccggat
      301 acctgcccgg tgcatctgct gccttatctc gcctgggcat tttccgttga ccgctgggat
      361 cgcaactggc cggaagagac aaagcgacag gttattcgtg atgcatggct gatacaccga
      421 cacaaaggga ccatcagcgc actgcgcagg gccattgaac cactgggata cctcattcgc
      481 gtgtctgagt ggtgggagtt cggaggagaa ccgggaacat ttaccgttga agtcggcaca
      541 ctggacagtg gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat tgctgatgcc
      601 cgtccggtca gccgccacat gacagggctg aatatcattc aggaaattcc gggggatatt
      661 ttcgcagcgg cggcaactta tgacggtgaa gttattacca tttatccaag cgattaagta
      721 tgagtaccac aacacgaaaa tttaaaaccg ttatcaccga tacgggtgca aaaaaattag
      781 cgcaggcagc cgcgccagat ggtaatcctg tccgcctgac tcatatggcc gtgggcgacg
      841 gtggcggcac gttgcccaca ccagacagta agcagacccg tctggtgcat gaggtgtggc
      901 gacacactgt taatcgcgtc atcctggacg caacacatca gaaccgcatt attgcggagc
      961 tggttattcc tcctgaaacg ggcggattct ggatccggga aattggtgta tttgatgagc
     1021 acggcgattt aatcgcggtg ggcaatactg ccgaaagtta caaaccaacc gttgccgaag
     1081 ggtccggacg tgcacaaaca tttcgcacca ttctgaccgt atccagcact gccaccgtgg
     1141 cgcttaccgt ggataacacc atggtgatgg ccacagtgga ttacgtggat gacaaactga
     1201 aagagcatga acagtcacga cgtcacccgg atgcctcgct gaccgcaaaa ggctttgttc
     1261 aactcagtag cgccactaac agcgattctg aaacgctggc tgcaacgccg aaagcggtta
     1321 aggccgcgta tgacctggct aacggaaaat ataccgctca ggacgccacg acggcacgaa
     1381 aagggatagt ccagctcagc agtgcaacca acagcacgtc tgaaacgctg gcagcgacac
     1441 caaaagcggt taaggcggta atggatgaaa cgaacaagaa agccccatta aacagcccgg
     1501 cactgaccgg aacgccaaca acaccaactg cacaacaggg aacgaataat acccaaatcg
     1561 caagcacggc tttcgttatg accgcgattg ccgcacttgt agactcttca cctgacgcac
     1621 tgaacacgct gaacgagctg gcggcggcgc tgggcaacga cccgaatttt gcgaccacca
     1681 tgactaactc gcttgcgggt aagcaaccga aagatgccac cctgacggcg ctggccgggc
     1741 ttgctactgc agcagacagg tttccgtatt ttacggggaa tgatgttgcc agtctggcaa
     1801 ccctgacaga agtcgggcgg gatattcttg cgaaatcgac cgttgccgct gttatcgaat
     1861 acctcggttt acaggaaacg gtaaacaggg ctggtaacgc cgtgcaaaaa aatggcgata
     1921 ccttgtccgg tgggcttact tttgaaaatg actcaatcct tgcctggatt cgaaatactg
     1981 actgggcgaa gattggattt aaaaatgatg ccgatggtga cactgattca tacatgtggt
     2041 ttgaaacagg cgacaacggc aatgaatatt tcaaatggag aagcaaacaa agcaccacaa
     2101 caaaagacct gatgaatctt aaatgggatg ctttgtctgt ccttgttaaa gcccttttca
     2161 gcagtgaagt aaaaatatcg acagtcaatg cactaaggat atttaattca tcttttggtg
     2221 ccatttttcg ccgttctgaa gaatgcctgc atatcatccc gacacgagag aatgagggag
     2281 aaaatggtga tatagggcca ctacgcccct ttacgcttaa tcttagaact ggtcggataa
     2341 gcatggggca tggtcttgat gttacagggg atatatttgc aaaccgtttt gcaattaaca
     2401 gtagtaccgg catgtggatt catatgcgtg accagaatgt tattttggga cgcaatgcgg
     2461 tatccaccga tggtgcacag gcattacttc gtcaggacca tgctgatcgc aaatttatga
     2521 ttggtgggct gggaaataag caatttggca tctacatgat taataactca agga
//
LOCUS       DAHBHC010000229         2574 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHBHC010000229 DAHBHC010000000
VERSION     DAHBHC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.234"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            135..341
                     /locus_tag="M2A35_005272"
     CDS             135..341
                     /locus_tag="M2A35_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7201414.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            367..906
                     /locus_tag="M2A35_005273"
     CDS             367..906
                     /locus_tag="M2A35_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCB7201415.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            969..1202
                     /locus_tag="M2A35_005274"
     CDS             969..1202
                     /locus_tag="M2A35_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCB7201416.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1268..>2574
                     /locus_tag="M2A35_005275"
     CDS             1268..>2574
                     /locus_tag="M2A35_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCB7201417.1"
                     /translation="MSVTEFKAKTGRKSSRKPSKEQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWSVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTTRQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQTVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDDDRTEQVQEKASVEEISLPLLTKMSS"
BASE COUNT          591 a          665 c          827 g          491 t
ORIGIN      
        1 cgtttcgcaa accgcgcttc acgtcgttgc ggcgggtccc tgcccgttgt tctgtccggt
       61 gccattcacc tgtttaagcc cctgtcgccg ggggctgata cagccacaac cgtctccgcc
      121 agtccagggg tgaaatgtac atcccgcaaa actttttgcc caaaagcagg caaaaacttt
      181 tgcggtctgc ccccggacag tcgtttccgg ccgtggcagt gatgcccctt gcggcgaagg
      241 ttctcaaaaa ggtgatggtg aataccggag ggaacaaaag gcaggtcccc gcaaaacgtc
      301 aaaaccgcgg ctcccgaaac tcaaaagatg atgggggctg aaaacatcaa cagaaggagg
      361 cacatcatgg cagttcgtgg cattaacaag gtcattctcg tgggacgcct gggaaaagat
      421 ccggaagtcc gttacatccc caatgggggc gcagtggcaa acctgcaggt cgccacgtcg
      481 gaaacctggc gtgacaaaca gacaggggag atgcgggagc agacagaatg gcatcgcgtg
      541 gtgctgttcg gcaagctcgc ggaagtggct ggcgaatatc tgcgcaaggg cgcgcaggtc
      601 tacatcgaag gtcagcttcg cacccgtagc tgggaggata acggtatcac ccgttacgtc
      661 accgaaattc ttgttaagac cacgggcacc atgcagatgc tgggacgtgc cgcaggtgct
      721 cagactcagc cggaagaggg gcaacagttc agcggtcagc ctcagccgga accacaggcg
      781 gaggccggta cgaaaaaagg tggcgcaaaa acgaaaggcc gtggacgtaa ggccgcgcag
      841 ccggagcctc agccgcaacc gccggagggt gacgattacg ggttttcaga cgatatcccg
      901 ttctgatcgg gctgactgtg ataaccgccc cgccccgttc tgtgcggggc atcaccggag
      961 agatgaggat gagcgaatat ttcagaatac ttcaggggct gcctgacggt ccttttaccc
     1021 gcaaacatgc cgaagctgtt gccgcacagt accggaacgt ctttatcgag gatgaccacg
     1081 gcgaacagtt tcgcctggtt gtccgtaata acggtgcaat ggtctggcgt acctggaatt
     1141 ttgaggacgg tgccgggtac tggatgaacc atgtcatcag ggatttcggg attcttaagt
     1201 aagagaggtg ccggacggga acgcgtccgg cagacaatca ttattaccgt tttgggaggt
     1261 actaactatg tcagttacag agtttaaggc aaaaacgggg cgtaaatcca gccgtaaacc
     1321 gtcaaaagag caggagactg cactgtctgc cctgctggcg cagacggagg aagtgagcgt
     1381 gccactggct tcgctgatta agtcaccgct gaatgtgcgc acggtgccgt attctgtgga
     1441 gtccgtcagc gaactggcgg agtccattaa aggagtcggc ctgttgcaga atctggttgt
     1501 tcatgccctg cctggtgacc gccacggtgt cgccgcaggt ggccgccgac tggcagcact
     1561 caacatgctg gcagagcgtg gcatcattcc ggcggactgg tctgtccgcg tgaaaattat
     1621 tccgcaggag ctggcgactg ccgcatcaat gactgaaaac ggtcatcgtc gggatatgca
     1681 ccctgccgaa cagattgccg gattccgcgc aatggcgcag gaaggcaaaa cacctgcaca
     1741 aatcggtgat ttgctgggct attcaccccg ccacgttcag cgaatgctga aactggctga
     1801 ccttgccccg gttatcctcg atgcgctggc agaagaccgc atcaccacag agcactgtca
     1861 ggcgctggcg ctggagaacg acaccacgcg tcaggtgcag gtgtttgaag cggcctgcca
     1921 gtcaggatgg ggtggaaaac cggatgtgcg ggttatccgc aatctgatta cggaaagtga
     1981 agtggcggtg gcggggaaca gtaaattccg cttcgtgggg gctgatgctt tctcgccaga
     2041 tgaactgcgc accgatttgt tcagcgatga cggggacggt tatgtcgacc gcgtggcgct
     2101 cgatgctgcc ctgctggaaa aactccagac tgtcgctgaa caccttcggg aagccgaagg
     2161 ctgggaatgg tgcgccggac gcatggagcc tgtcggtgag tgccgtgagg atgccggaac
     2221 ataccgctgt ctgccggagc cggaagcggt gctgacggag gcggaagaag aacgcctgaa
     2281 cgaactgatg gcacgttacg acgcgctgga aaaccagtgt gaggaatccg acctgctgga
     2341 agcagaaatg aagctgatgc gctgcatggc gaaggtcaga gcgtggacgc cggagatgcg
     2401 tgccggaagc ggtgtggtgg tgtcctggcg ttacggtaat gtgtgtgtcc agcgtggtgt
     2461 gcagttgcgc agtgaggatg acgcggctga cgatgatgac cgcacggaac aggtgcagga
     2521 aaaagcgtca gtggaggaaa tcagtctgcc gctgctgacg aaaatgtctt ccga
//
LOCUS       DAHBHC010000230         2552 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHBHC010000230 DAHBHC010000000
VERSION     DAHBHC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.235"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            122..1450
                     /locus_tag="M2A35_005276"
     CDS             122..1450
                     /locus_tag="M2A35_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCB7201418.1"
                     /translation="MSHRELFSEFLENLNLDLKQAKKISYHYRKITKSLNLAFRGTSS
                     RVANRLKVGSVGRHTAIKGISDLDMLYIMPPNQYEYYNRKDNGQSALLTDVRNILAEE
                     YPDQTVKKDRLVVQIIFKNFYVEVQPVFRQDDDSFKFPESYNGGAWRITKPLHEKAAM
                     TAFSRDKSNNLRKLCKMIRAWKNLHGVNMGGLLIDTLAYRFLSSTSDYDNTGNGSLGA
                     LARDFFEYLSNEERKERYLALGSNQHVRVKSPWFGRAAKHAYELCCDALDAEGAASEN
                     DRWRKVFGRAFPRRKVGIMEARLGLESHAADAVPWTDTEEFIEDKYPVDIRYSLNLDC
                     TVTQDGFPPRSLREMLTRRFRLSARKSLLFRADLTEMEAEEPYTVMWKVLNVGDEARR
                     RNMIRGQIVSDGGYCTKKETTDFRGDHMVECYVIKNEVVVARAQIEVPIS"
     gene            1458..2033
                     /locus_tag="M2A35_005277"
     CDS             1458..2033
                     /locus_tag="M2A35_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCB7201419.1"
                     /translation="MTQSEMLKLIARHGYNVGFGAKKNFATYDLICKASGWISFISLA
                     VGVFALFIPELATNVISAILIIFGVATMYIQFYDSEKQSYESAGIEQTKIFNELEVIY
                     RNVKSDANFDYQRTQQSIDAIMSRFYGTVISKQIFGSNWYAHYKFYCEFEKAWVEEEL
                     NLTLKDKIPLSLCFLAGLAIIFTIAVIWKTP"
BASE COUNT          739 a          557 c          591 g          665 t
ORIGIN      
        1 actccgttaa cgcttacgtt taagtcataa agccatgcag aacgctcacc tcccaagatc
       61 ttggttttta taaggtgtaa tgataatgta agctaatgtt tcacatgcca aaggaaacac
      121 catgagccac agggaattgt tcagcgaatt tcttgaaaat ttgaatcttg acctcaaaca
      181 ggcaaaaaag atcagctatc actatcgaaa aataaccaag tcattaaatc ttgcgttcag
      241 aggaacgagt tcccgggtag caaaccgctt gaaagtgggg tcagtaggca gacataccgc
      301 aataaaaggc atatccgatc tggatatgct ttatattatg ccccccaatc agtatgaata
      361 ttataaccgt aaggataatg gtcagtctgc attgcttaca gatgtcagaa acattctggc
      421 ggaagaatat cctgaccaga ctgtgaaaaa ggacagactg gttgttcaga tcatctttaa
      481 aaacttttat gtcgaggttc aacctgtatt cagacaggat gatgacagct ttaaattccc
      541 tgaaagctat aatggaggag catggcgcat tacaaaacct cttcacgaaa aggcggccat
      601 gaccgcattt tcgcgagata aatccaataa tctgcgcaag ctgtgtaaaa tgatcagagc
      661 ctggaagaac ctgcatggcg tcaatatggg tggcttactg atagacacac tagcgtatcg
      721 ctttctttca tcaacatcag attatgacaa taccggaaat ggcagcctgg gagccctcgc
      781 cagagacttt tttgaatatc tgtcaaatga ggagagaaag gaaaggtatc tcgcgttagg
      841 cagtaatcag catgtacgtg ttaaatctcc ctggtttggc cgggcagcga aacacgcgta
      901 tgaattatgt tgtgatgcct tagacgctga gggggctgcc agtgaaaatg accgctggcg
      961 caaagtgttc ggacgagcct tccccagacg taaagtgggt attatggaag cccgtcttgg
     1021 tctggaatcg catgcggcag atgctgttcc ctggaccgat accgaagagt ttatagagga
     1081 taaatatccg gtggatatca ggtactcact caaccttgac tgtaccgtaa cccaggatgg
     1141 tttccctccc agaagcctga gggagatgct taccagaaga ttccgcctct ccgctcgaaa
     1201 atctctcctt ttccgggcag acttgacaga aatggaggca gaggaaccct acaccgttat
     1261 gtggaaggta ctgaacgtgg gtgacgaagc acgcagaaga aatatgatcc ggggacaaat
     1321 cgtttcggac ggcggctact gtacgaagaa agaaactacc gacttccgcg gagatcacat
     1381 ggtagaatgc tatgtgataa aaaatgaggt tgtggttgcc cgggcccaga ttgaagttcc
     1441 cataagctga ggtttttatg acgcaatcag agatgctgaa attaatcgcc cgacatggtt
     1501 ataacgttgg atttggcgct aagaaaaact ttgccaccta tgatctgatt tgtaaggcat
     1561 ccggttggat tagctttatt tctcttgctg tcggtgtgtt cgcccttttt attcctgagc
     1621 tggccacaaa cgtcatttcc gcgatattaa ttatatttgg tgttgctaca atgtacattc
     1681 agttttatga ctcggaaaag cagagttatg aaagtgcggg aattgagcag accaaaattt
     1741 ttaatgaact tgaagtcatc taccgcaacg taaaaagcga tgcaaacttt gactatcagc
     1801 gtacacaaca atccatcgac gctatcatgt cccggtttta cggcacggtc atcagcaaac
     1861 agatcttcgg aagtaactgg tacgcacatt ataaatttta ctgtgaattt gaaaaagcat
     1921 gggttgaaga agagctgaac ctgacactga aggacaaaat cccgctttcg ctctgctttc
     1981 tcgccggtct ggcgatcatt tttactatag ctgtcatctg gaaaacgccc tgatgctgca
     2041 gccaatgaaa aattcagcaa gctgactgtc aaatatgcga tgtatagccg gtatcaccat
     2101 ccagtgtacg ttctataagg ggggcgcttg gaaaacgggt agagtcaaga tgtggcgcgg
     2161 taattttaca ggtcaggcat atctgttgcc gcctctttcg attggcggtg ccttactgtc
     2221 ctgaccataa atcctgtttc cacaccccgc tcatcgaagc ggacatgcgc ttctaacaca
     2281 tccggctctc ggacagaatc atgccttcgc ccacggaaac ctctgcggga gcttcggtgc
     2341 atattttcat ggaaggggat cacccaataa cttccatcga gatcgggtca taacggccaa
     2401 tccgtgatcg aatagagcgg aatacgcact ctgacgaact gcttacattt aaactaatta
     2461 caaactatat aaatccgcct tgctcattca cagggtagat atattcttac ttttaatttt
     2521 atgtgtaatt aactgatgac gaaaaatgaa tt
//
LOCUS       DAHBHC010000231         2549 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHBHC010000231 DAHBHC010000000
VERSION     DAHBHC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.236"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1189
                     /gene="nleA"
                     /locus_tag="M2A35_005278"
     CDS             <1..1189
                     /gene="nleA"
                     /locus_tag="M2A35_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HCB7201420.1"
                     /translation="AHPPHPAPNQIPESELPPGCETGFVVNIPEDIQQHTPECGETTA
                     LLGLIRDKGLLSGLDAYLAPHLEEGSAGKKALDMFGLFNVTQMALEIPSTVPGISGKY
                     GVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPLQKFLSQNPNPWFGVTAQV
                     VRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVIRPGEFDLKYGSTPLQSTL
                     GLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQYTGTDAMLSPHVGSMAGQ
                     ALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFP
                     NKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQE
                     TRV"
     gene            complement(1526..2101)
                     /locus_tag="M2A35_005279"
     CDS             complement(1526..2101)
                     /locus_tag="M2A35_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HCB7201421.1"
                     /translation="MPLTSDIGSHSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TDGSFSSSGGNGGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQEGIQSKISQYK
                     FSVCPERLQCPPESAQCPITLEIPEEGVFIKNSGDSVVCSLFDVTAFSRLVSEKSPHP
                     LTREKLTASMVVSADKCFYDHGKGSFVIKDS"
     gene            2258..2524
                     /locus_tag="M2A35_005280"
                     /pseudo
     CDS             2258..2524
                     /locus_tag="M2A35_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          798 a          572 c          483 g          696 t
ORIGIN      
        1 tgcacaccca ccacatcctg cgccaaatca gataccggaa tcagaattgc cacctggatg
       61 tgagactgga tttgttgtta atattccaga ggatatacag caacacactc cggaatgcgg
      121 tgaaacaaca gctctgttgg gtttaataag agataaaggt ctgctctcag ggttagacgc
      181 atatcttgct cctcaccttg aagaaggctc agcaggaaaa aaagcattgg atatgtttgg
      241 tttattcaat gttactcaga tggcattaga aatacccagc accgttcctg gtatctctgg
      301 taaatatggt gttcagatga acattgtaaa accagatatt caccccacaa ccgggaatta
      361 ttttttacag ttattccccc tacatgatga ggtgggaata aacttcggtg cgctcccagg
      421 accactccag aagtttctct ctcaaaatcc caatccatgg tttggagtaa cagctcaagt
      481 tgttcgtaac cacggcatag aactacctat agtcaaaaca gataatggat ggaaactagt
      541 tggagaaaca ccacttacac ctgaagggcc taaagcaaat tatacggaag aatgggtcat
      601 cagacctggt gagtttgact taaagtatgg cagcactccg ttgcagagca ctttaggttt
      661 agaatttgga gctcatttta aatgggattt agataatcct aataccaaat atgccgttct
      721 taccaatgca cttgcgaatg cagctggtgc tgcaggggga tatactctat ctcaatatac
      781 aggaacagac gcaatgctga gcccccatgt tggctcgatg gccggacagg ctttggggca
      841 tgctgtacaa tgctacactc caggattaaa gccagacact attttatggt gggcaggtac
      901 gactctgggg ctggcagatt taaacaaagc cgagacaggt gaaggcagat tcactgacta
      961 tcctcgtata tggtggcacg caagagaagg tgccatcttc cctaataaag aagatattgc
     1021 ccgtgcaaca ggggctgata tacgagctat ggaagaaggt atccctgttg gacagcggca
     1081 tccaaatcca gaggatgtgg taatcgatat cgaaagcaat ggcttaccac atcataatcc
     1141 atcaaatcat gttgatatct ttgatataat ccaagaaaca agagtctaag ctttaacttg
     1201 tttattttaa aataatagct gaaatggatg gcagctatta ttaataagga ttaatatatt
     1261 ttttctaaaa taccccacaa aataaacaca ccataaaaat tctaaataca cacacctaat
     1321 ctcaaaaaaa caaacttact atttgacttg gattctgttt ttaacccaat attttccgac
     1381 agcatctcca cagaaaaatg catttacgta aagacgccct caagtcatct gaaacctgtc
     1441 aaccacaggc atactcttgt attcttgatg cctgtggcgt ttttacaaat tactgacagc
     1501 taatattcgc tcagagaaaa atattttaac tatcttttat aacgaagcta cccttaccat
     1561 ggtcataaaa acatttatca gcacttacaa ccattgatgc cgttagtttc tctcgtgtca
     1621 gtgggtgagg tgatttttca ctaactaacc gagaaaatgc agtaacatca aataaactac
     1681 atacaactga atcccctgaa ttcttaataa acaccccttc ctctggtatc tccaatgtaa
     1741 tgggacattg agcagactct ggcgggcact gaagtctctc tggacaaaca gaaaacttat
     1801 attgagatat tttgctctga ataccctctt gcgattgtgc ttcacctgag tccgaaagca
     1861 tacttagcat tctctcacta agggctcgag gaccattatt aaatcccaaa atcaataact
     1921 cagaaagcaa tccgccatta ccgccactgg atgaaaagct cccatcagta gcatcataca
     1981 caatactgac agtttcacca cctactgtaa tatctccgcg cccattggct gcaattcggg
     2041 cacgaaaaac ctccagccca agattaaatg aatgtgatcc aatatctgag gttaatggca
     2101 taacaaaact ccctatttta atttgaactc cagacttaaa tagctgtaac aaacatctgc
     2161 cttatatggc aacacaaaaa ccggagccgg actccgtttt ttgtgtgtcg ggctgttcat
     2221 tctatccttt caacattccg tctgattggc atttgtaggt ccagcaacag tcagtcacga
     2281 tgttagttat cttggcagtg ttctggatgc agccaaacct atatacggaa ttaattacac
     2341 atcaaacccg gcgaaaagtg ctcgtccata tctacttaaa cttgctttga ttggtaaatc
     2401 aaaccgtcgt aatcgtagac cagcagttga tgaacttgac atgctcattg aagcccttca
     2461 acaacgatct actcataaat gttcaaaaat tccgttcgtt gatatcctca aaagttcagc
     2521 ctaggatatg ctgaaccggt tttcctgaa
//
LOCUS       DAHBHC010000232         2519 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHBHC010000232 DAHBHC010000000
VERSION     DAHBHC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.237"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..2123)
                     /gene="ag43"
                     /locus_tag="M2A35_005281"
     CDS             complement(<1..2123)
                     /gene="ag43"
                     /locus_tag="M2A35_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCB7201422.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSLPVLAADTVVQAGETVSGGALENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAMNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGKATAVTQNTGGALVTSTAATVTGSNRLGNFSVE
                     NGMADNVVLENGGRLDVLSGHTATNTHVDEGGTLDVRSGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGNALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNMLQ
                     AGAFNYSLNRDSDESWYLRSENAYRA"
BASE COUNT          481 a          750 c          658 g          630 t
ORIGIN      
        1 gcacgataag cattttcact gcgcagatac cagctctcat cactgtcccg gttgagggag
       61 tagttaaagg ccccggcctg cagcatattc ccctggacaa aggccccttc ctccgtggtg
      121 gcaccgttaa tggcttccac cacctgaatg cccttaccgc tggtcgccag ccccgacgca
      181 ctgttgccgg cgttcaccag gttcaggatg gtttttccgg ttgccctgcc accgtcaatg
      241 accagtctgt cagcattgtt ctgtgccata tccgggcgta cacgcaggct gatggtgcca
      301 ttctgcccgt tcaggttttt cactttcagg gttgccggta cgaacttccc tgtgcgggtg
      361 gaggtgaaat gaatctgtcc ggcatggctg aggtcatcca ccaccgactg caccgtggcg
      421 ttatcaggga tattccaggt ggcaccggag gcgagagtga cattcgtggg gtcaatggca
      481 ccgttcagca cagtgctgcc ggtcagcttc agggctgtac cgcgctgagc aatgacatcc
      541 gtggtgacgg tactgtcgtt cagcgtcagc gtgccttcat tcaggttgac ggctttctgg
      601 gtgagtgtgg tgttgctgac agtgagtgtg ccacttcctg atttttccac gctgccgtta
      661 ccggtgagag agcctccctg caggagtgca ttgccttcac ggatggtcag ggtatcgttg
      721 ttcaccgtct tcccggaaag ggtaagtatg gcgccgttat tcagcgtggt ggtgcccgcc
      781 agtgtgccgc ccctggcggt gaacagtccg ccatttaccg tggtgtccgt ggccgtatca
      841 ccggcgttca gcgtgaatga actgcctttt tccagcatca gggcatccgc ctgaccgcct
      901 ccgatacgga atgccgttcc gtcgctccgg gtaccactga cagcggcacc ggaatcggcc
      961 agcagtacac cgccattccc catggatacg gtggtggctg tgccaccact gcggacatcc
     1021 agggtacccc cctcatccac atgtgtgttt gtggcggtat ggccgctcag tacatccaga
     1081 cgtccgccat tttccagcac gacgttatct gccatgccat tttccacgga gaaattcccc
     1141 agacggttgc tgccggtgac ggttgccgcc gtactggtaa ccagtgcacc gcccgtgttc
     1201 tgggtgactg ctgtggcttt acccccggcg tccacctgca gtctgccctt ctgattaacg
     1261 gtggtattcc cggccacacc cccgtttttg acaatctgcc agccgtcact gttcacaaca
     1321 gtgtcagacg ctgtaccgcc gttgtgcaca tactggtatc ccccattcag cgtggtatcc
     1381 agtgcgtgac catgtacagt ctggtcgccg ccggcataaa ccacagtggt atttgccgtt
     1441 ccttcagcag ccacaatctg acgaccattt ttattgatgg tggtgcgtac ggcatctccg
     1501 tagacggtct gcccggtatc accattttcc gcatccggtc ccccttccgc gccggtattc
     1561 acaaccgtgt cggttgccac tgcgccggat ttgacggcct gccagccctt ctcattaatg
     1621 acggtacccg ttgcaatccc gccttcatgt acccactgct caccgccgtt cagagtggta
     1681 ttcattgcct gcccctgaag gctctgtccg cctccggcac tgataaccgt gtctgaaacg
     1741 cttcctccgg cattcactct ctgaagacca ccaccggtga cagtagtgtt gttggcgata
     1801 ccgccatttt gtatccattg cccgccggta ttggcctcgt tatccggccc atactccaga
     1861 ccggtactga tggtcattcc gttggctgta ccgaagacaa tctggttgtc atgattttcc
     1921 agtgctccgc cgctcacggt ttctcccgcc tgtacaaccg tgtcagcagc cagtaccggg
     1981 agtgatgtga cagcagcaag agacagcgca accgccacac cggcgcgttt tccccgtgag
     2041 cgggccagtt cggaggccac caccagggtg cccgtaatgt gattccatac cagcctgtag
     2101 ctggtgttca gatgtcgttt catcagcttt tccttacaga gggtgaataa aaaagccggt
     2161 acccacaaat gtggataccg gcaggtacaa aacagcatgg tcagataaag tggtttttcc
     2221 cggacataaa tgcttcccct ctctcatccg ccagagaagt atcagtccgt tgtgtgcaat
     2281 tccgttctgc atgatgaaga gattcaactg ccttttcgca gaccattaac cactataaac
     2341 gatcgatagt aacgatcgat acctgtttta tcgatcgttt tattctatta acagcagagc
     2401 agtcacgcaa gagaaacagg aggggcatga ggtgtttgtg tcatgttttc agttggttaa
     2461 cgcaatgtaa tcaggcattt cccctggcag agtgttcaga agaaaatcca gttccatac
//
LOCUS       DAHBHC010000233         2496 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHBHC010000233 DAHBHC010000000
VERSION     DAHBHC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.238"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..469
                     /locus_tag="M2A35_005282"
     CDS             <1..469
                     /locus_tag="M2A35_005282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201423.1"
                     /translation="KKLTRIHQETIVDEDGIQKLHTSRESFTIPAEPPYVKLYLADIG
                     AIHGLSKSQSDVLFQLAMLIGWDGIVSVSKTRFEKTIKPRVGIEYQSFKNIVAKLVEK
                     GIFLRSGRGELEANPFIFARGDWPSIYERRKSIELTITYDYQGRSFKSKLLNK"
     gene            487..705
                     /locus_tag="M2A35_005283"
     CDS             487..705
                     /locus_tag="M2A35_005283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201424.1"
                     /translation="MNSEDKLIIKVDRLITHDSLNQQNILSEWERRFLLSILGIHRKI
                     NENSLFGLSPKQKKATFNILKKHQFHVD"
     gene            complement(739..1008)
                     /locus_tag="M2A35_005284"
     CDS             complement(739..1008)
                     /locus_tag="M2A35_005284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201425.1"
                     /translation="MKTVSRIERGYCRISARIAFALHFVYGVYRNIHTEHQIQEFEKK
                     ERERQQMFRESEAAAAAERRQQRQSRPSDPRLTHGSGKRKKKKRR"
     gene            complement(1709..1936)
                     /locus_tag="M2A35_005285"
     CDS             complement(1709..1936)
                     /locus_tag="M2A35_005285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201426.1"
                     /translation="MTADDRSIPESIHQSFSQLRDYLDAFSCLSDMIPLGEPDRFDFE
                     SLVYLLRMVSESLYVSLDVHYSTLLAEFVKR"
BASE COUNT          799 a          519 c          533 g          645 t
ORIGIN      
        1 aaaaaaatta actcgcattc atcaagaaac tattgttgat gaggatggaa tacagaaact
       61 tcatacatct agagaatcat ttacgatccc ggcagaaccg ccttatgtga aattatatct
      121 tgctgatatc ggcgctattc atggcctatc aaaatcccaa agtgatgttt tattccaatt
      181 agccatgttg attgggtggg atgggatcgt atcagtatca aagacaagat tcgaaaaaac
      241 gataaagcca agagttggta tagaatatca atctttcaaa aacatagttg ccaaattagt
      301 tgaaaagggg atttttctgc gttcagggcg tggtgaactt gaagctaacc cttttatatt
      361 tgcaaggggt gactggcctt caatatatga gagaagaaaa agtatagaac ttacaataac
      421 atatgattat caaggaagga gttttaaatc aaagctttta aataaataaa taaaaggtga
      481 ggacagatga attctgaaga taagctgatt ataaaagttg ataggttaat tacacatgat
      541 tcattaaatc aacaaaacat tttaagtgaa tgggaaagaa ggtttttatt aagcattttg
      601 ggaatacata gaaagattaa tgaaaattct ctatttggat tgtcaccaaa acagaaaaaa
      661 gcaacattta atattttgaa aaaacatcaa tttcatgttg attaaacaat ttagtaataa
      721 gcaataccaa agagcgcatt accggcgctt tttcttttta cgttttcctg agccatgagt
      781 aagacgagga tcagatggtc gtgactgcct ttgttgcctg cgttctgctg ctgcagccgc
      841 ttctgattca cgaaacattt gttgacgttc acgctctttt ttctcgaact cctgtatctg
      901 atgttctgtg tggatgttcc tgtaaacccc gtacacaaaa tgcaaagcaa aagctatgcg
      961 ggcgctgatg cgacaataac cgcgctcaat cctcgatacg gttttcacat ccacatctgg
     1021 acattttcca tatcacctgt agcgagagtc ttaaaccaaa tttatccagg tggtcatgcc
     1081 cccgaagtgt tgacacactt gcgggggttt tactttcaac cacaaagaac aaaatacaca
     1141 caaacccatt tcggagcaaa tccacgctca gcccttgcgc cacaagggct agaagcgaat
     1201 ttgaatccta gagttatccc ttaagtataa atacaaggaa gcaaccagac cggaagacta
     1261 aaaaccgtag gaatgcacgg tgaatatcgg gtgtaacaga actcccgaac cgctgcgcgg
     1321 tcattttctg gcaatctgta acggcagacg acgaatcatc atcatcgtga tcgtcgtccg
     1381 tcgtcacggc aggcgctccc gtcctcgacc ctgcgggcgg gtgcggctgg cgttcctccc
     1441 tcctccttcc cttttcttct tcatcgacgt gctggcgtaa cgttgaaatg ctggaaagcc
     1501 aagtcctgag cggcttcgag aagcaaactt aagtggtttt cggttgagtt gttactatat
     1561 agacaaggaa gggagccgcc tgactttacc attactaaaa ccggcttatc agggtgatgg
     1621 cccactttgt gaaccatcac ccgacacaag gatattttta cggagtgtcg tcaatcacta
     1681 aatcggaacc ctaaagggac cccacgattt agcgtttgac gaattcagcc aagagagtgg
     1741 agtaatggac atcaagagag acatacagag attcagacac catccgaagt aaatatacaa
     1801 gtgattcaaa gtcaaacctg tcaggttcac caagcggaat catgtcggaa aggcatgaaa
     1861 aagcatcaag ataatcacga agctgagaaa aggattgatg tatagactca gggatggaac
     1921 gatcgtcagc agtcatatag acctccgcta tcaaggttta tgtgattaga aacgccctgt
     1981 agtcctacct acggggcgtt ttgccattag atcacccata accactacct gcaagaaaaa
     2041 actcatcaca gactcattca ggccgtttag agacaaaaat cagattctcg ggaatagaaa
     2101 acatcagaga gatgaatccg ctcgccgcag acctagcgac cgaacgttag tgagcgagtg
     2161 gtcgaggaag cggaaatgct accgaattga cataaagcaa gtacgcacaa aggcttattt
     2221 tgagcaatcg tagatcctta atgccttcct taatatatag atatagatat gcaaagccag
     2281 agatgacaag ggctacatta aagaatcgta ctcacctcgg gtactaatcg tactcacctt
     2341 gagtactaat cgtactcacc tcgggtacta atcgtactca cctcgggtac taatcgtaat
     2401 tgacattgag tactataggt tgtagcttgt tggtacttca atacaagtac tatgtgagat
     2461 ggagagctag ggaacgatgc aaacaagcat gcaaat
//
LOCUS       DAHBHC010000234         2488 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHBHC010000234 DAHBHC010000000
VERSION     DAHBHC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.239"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(131..868)
                     /locus_tag="M2A35_005286"
     CDS             complement(131..868)
                     /locus_tag="M2A35_005286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201427.1"
                     /translation="MALLFAADACLVASSFEADADDADDAAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAVAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALDDA
                     FVSDDFAASFEADASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDVFAAPALVAAAL
                     FEDSAAAALFDASVAFADAVPALEDAD"
     gene            999..1121
                     /locus_tag="M2A35_005287"
                     /pseudo
     CDS             999..1121
                     /locus_tag="M2A35_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1206..1388
                     /locus_tag="M2A35_005288"
                     /pseudo
     CDS             1206..1388
                     /locus_tag="M2A35_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1899..2488
                     /locus_tag="M2A35_005289"
                     /pseudo
     CDS             1899..2488
                     /locus_tag="M2A35_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          661 a          690 c          692 g          445 t
ORIGIN      
        1 acacggcagc cgcgaagaag tcagccagtg atgccagcac atctgccagt gaggcggcaa
       61 cccatgcgac tgatgctgca ggctcagcac gcgcagccag cacgtcagcc ggacaggccg
      121 cgtcgtcggc tcagtctgcg tcttccagcg caggaacggc atcagcaaag gccactgaag
      181 catcaaaaag tgctgccgct gcagagtcct caaaaagcgc ggcggccacc agtgccggtg
      241 ccgcgaaaac gtccgaaacg aacgctgcag cgtcacaaca atcagcagcc acttctgcat
      301 ccaccgcgac cacgaaagcg tcagaagctg ccacctcagc ccgggatgcg tcggcttcaa
      361 aagaagcggc aaaatcatca gaaacgaacg catcatcaag cgccagtagt gccgcttcct
      421 cggcaacggc ggcaggaaat tccgcgaagg cggcaaaaac gtccgagacg aacgctaagt
      481 cttctgagac ggcagcggga cagagcgcct cagctgcggc aggctcaaaa acagcgactg
      541 cgtcgtctgc cagtgccgcg tcaacaagtg ccgggcaggc ctcagccagt gccaccgccg
      601 ccggaaaatc ggcagaaagc gccgcatcat ccgcttcaac agccacaacg aaggctggcg
      661 aagccactga acaggccagc gcagcagcga ggtctgcttc cgcagcgaag acatccgaga
      721 cgaacgcgaa agcgtcggaa accagcgcag aatcctcaaa aacggctgcc gcatcgtcag
      781 ccagttcggc ggcgtcatcg gcatcatcgg cgtctgcttc aaaagatgag gcgaccagac
      841 aggcgtcagc agcgaagagc agcgccacga cggcatccac gaaggcgaca gaggcagctg
      901 gcagtgcgac ggcggcagct cagagcaaaa gtacggcgga atccgcggca acgcgcgccg
      961 agacagcagc taaacgggca gaggatattg catccgccgt ggcgcttgag gatgcgagca
     1021 cgacgaaaaa ggggatagta cagctcagca gtgcgactaa cagcacttcc gagtcactgg
     1081 cggcaacgcc aaaagcagtt aaggccgcgt atgacctggc taacgggaaa tacaccgcac
     1141 aggatgcaac gacagcacag aaagggatcg ttcagctcag cagcgcgacc aacagcacat
     1201 ctgaaatgct ggcggcaacg ccaaagtcgg taaaggcagc ctatgacctt gctaacggga
     1261 aatataccgc tcagggcgct acgacagcac aaaaaggaat tgtccaactc agtagtgcaa
     1321 ccaacagcgc atctgaaacg cttgccgcga caccgaaagc agtgaaagca gctaatgata
     1381 atgcgaatgg tcgggtacct tctgcccgta aggtgaatgg taaggcgctt tcagcggata
     1441 taacactgac gccgaaagat attggtacgc ttaactcaac aacaatgtca ttcagcggtg
     1501 gtgctggttg gttcaaatta gcaacggtaa ccatgccaca ggagagttct gttgtttcaa
     1561 ttacgttgat tggtggcgcg ggatttaacg tggggtcacc tcaacaggca ggtatatctg
     1621 aacttgtttt gcgtgcaggt aatggtaatc cgaaggggat tactggtgct ttatggcagc
     1681 gcacatcgac agggtttaca aattttgcct gggtcaatac atctggtgat acttacgata
     1741 tttacgttgc aatcggaaat tatgcgactg gtgtaaatat tcaatgggat tataccagta
     1801 atgccagcgt gacgattcat acgtcaccag catattctgc taataagccg gaagggttaa
     1861 cggacggtac agtttattca ctctatacgc catcagagca gttttatccg cctggcgcac
     1921 caatcccgtg gccatcggat accgttccgt ctggttatgc cctgatgcag gggcagactt
     1981 ttgacaaatc tacctacccg aaacttgcag tcgcttatcc gtcaggcgtg atccctgata
     2041 tgcgtggctg gacgattaag ggcaagcccg ccagtggtcg tgccgtattg tctcaggagc
     2101 aggacggcat taaatcgcac acccacagcg ccagcgcatc cagtacggat ttgggtacga
     2161 aaaccacatc gtcgtttgat tacggcacta aatccacgaa taacaccggg gcgcatacgc
     2221 acagtctgag cggctctaca agctcggctg gagcacattc gcacgttgat ggtcgcagat
     2281 ttaatacaag cacttttaaa gacacctatc agtatggttc tacagctgtc ggaagtaact
     2341 cataccaggt acaaggggcg gttggtctgg gcgttggcac gatggcgaat acgaactctg
     2401 ctggcgcgca tacccactca ctgtccggca cagcagcatc tgcaggtgca cacgcgcata
     2461 ctgtcggtat tggtgctcat acgcactc
//
LOCUS       DAHBHC010000235         2461 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHBHC010000235 DAHBHC010000000
VERSION     DAHBHC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.240"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..785
                     /locus_tag="M2A35_005290"
     CDS             <1..785
                     /locus_tag="M2A35_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCB7201428.1"
                     /translation="RYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPL
                     EVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFM
                     QWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQ
                     YKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNNETVSY
                     FCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(751..873)
                     /locus_tag="M2A35_005291"
     CDS             complement(751..873)
                     /locus_tag="M2A35_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201429.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            1076..>2461
                     /locus_tag="M2A35_005292"
     CDS             1076..>2461
                     /locus_tag="M2A35_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCB7201430.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLS"
BASE COUNT          722 a          500 c          491 g          748 t
ORIGIN      
        1 accgttacag acgcagtaat tcaggtaacg gtacttttgc aaaagtgata gcagccatcg
       61 agcgtctgaa atcatatcaa gttgagttta atacgttaac cgtcattaat aacgttaatg
      121 tccattaccc tcttgaggtt tatcattttt taaaatctat cggcagtaaa catatgcaat
      181 ttatcgaatt gctcgaaacc gggacgccga atattgattt cagtggtcat agtgagaaca
      241 cattccgtat cattgatttt tctgtgcctc ccacggctta tggcaagttt atgtcaacca
      301 tttttatgca atgggttaaa aacgatgtgg gtgaaatttt cattcgtcag tttgaaagct
      361 ttgtcagccg ttttttgggg aatgggcata caagttgtat tttccaggag tcctgcaagg
      421 ataatctggt tgttgaaagt aatggagaca tttacgaatg cgaccatttt gtctatccac
      481 agtacaaaat tggaaacatt aataaatctg aactcaaaac gatgaacagt gtacaactga
      541 cagcgcaaaa aaaacggatt tcagcgaaat gtcagcaatg tgcatataaa cctatctgca
      601 atggcggttg tcctaagcat cgtattacta aagtaaacaa tgagactgtt tcttattttt
      661 gcgaaggtta taaaatcctt ttttcaacca tggtacctta tatgaacgcc atggtggagt
      721 tagctaagaa cagagtaccg ctttaccaca ttatggatgt tgcaaaacaa atggagaata
      781 attaataacg ctctctttta actccattct tagcctcctt cttatgacct taacaaggca
      841 cccgacataa attcctgtcg ggtgtttcgt catcatcatc ttcccttgcg aaaatcctgg
      901 cttacgtggt atgcgtcagg gcattagcaa tacccttgat tatcaatagc ataaccataa
      961 ccactgaaaa tgaaatattc ctttatattt catattgtta aatgatactt aaataaattt
     1021 catcagtaaa tcattgccat agataatcat tggatggcac aatcaatttt cgctaatgat
     1081 aaccattccc attacgtttt gtatgttgat agcaaagtat ctctgtctgc tgaagccctt
     1141 ttggctgcgt aagaataata aaacttcagt gttgttaatc attattatcc tcgccatgat
     1201 ccttggcgtg gttaaaattc aggtctggtt aaacgactgg aataatgatt ttttcaatgc
     1261 gctaagccag aaagaaaccg ataagctttg gcagcttgtc ctttggttcc ctgccctgct
     1321 ggggatcttt gtattaatat ccgtaaacaa aacatggtta attaaactac tgactattcg
     1381 ctggcgcgaa tggttaacag attactacct taatcggtgg tttgcagata aaaactacta
     1441 cttcacgcag atctacggcg agcataaaaa tacagataac cctgaccaac gtatcgctga
     1501 agacattctc ttactgatca gcaaaacgct cagcctttca tttggcttca tccagtcact
     1561 tagcatgctg atcaccttta ccgttattct ttggcaaagc gcgggtacgc tctcattcac
     1621 tgttggtgga acagaatgga atatccaggg gtatatggtc tataccgttg tgctcattgt
     1681 gatcggtgga actttattta cccataaggt tggtaaacgt attcgtccgc ttaatgtgga
     1741 aaaacaacgt agcgaagcga catttcgaac aaatcttgtg cagcataata agcaggcaga
     1801 actgattgcg ctaagcaatg ctgagtctct acaacgccag gagttgagcg acaattttca
     1861 taccattaaa gagaactggc atcgtttaat gaatcgtcag cggtggcttg attactggca
     1921 gaatatttat tcgcgctcgc ttagcgttct tccctacttt ctgttattgc cgcagtttat
     1981 tagcgggcag atcaatctgg gcggactgat gaaatcgcgc caggcattta tgctggtatc
     2041 gaacaattta agctggttta tttataaata tgacgaactt gctgagctgg ctgcggttat
     2101 cgatcgcttg tatgagttcc atcaactcac tgaacagcgc cctacgaata agcctaaaaa
     2161 ttgccaacat gcggtacaag tggctaatgc gagtattcgt acgcctgata ataagatcat
     2221 attagagaac ttgaactttc atgtttcgcc aggcaaatgg ctattactga aaggctactc
     2281 tggtgcggga aaaaccacac tgcttaaaac attatcccac tgctggccgt ggtttaaagg
     2341 tgatatttct tctcctgctg acagttggta tgtgtcacaa acaccgttaa tcaaaagcgg
     2401 cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata aatcgttgag
     2461 c
//
LOCUS       DAHBHC010000236         2431 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHBHC010000236 DAHBHC010000000
VERSION     DAHBHC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.241"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            98..706
                     /locus_tag="M2A35_005293"
     CDS             98..706
                     /locus_tag="M2A35_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCB7201431.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            851..1526
                     /locus_tag="M2A35_005294"
                     /pseudo
     CDS             851..1526
                     /locus_tag="M2A35_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1699..1935)
                     /locus_tag="M2A35_005295"
     CDS             complement(1699..1935)
                     /locus_tag="M2A35_005295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201432.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1937..2329)
                     /locus_tag="M2A35_005296"
     CDS             complement(1937..2329)
                     /locus_tag="M2A35_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCB7201433.1"
                     /translation="MCNGESINENKEYGGLICKKQGEYLPMNPISSNDNDSVDLRNIK
                     CPEGSERVGDYHPHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMEKKEYSSY
                     LGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT          664 a          572 c          484 g          711 t
ORIGIN      
        1 gaacttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg agcacgttgt
       61 ctgcgaccca ccgcttttta tacatggacg tttaactatg aaaaacaggc tgctgatcct
      121 cagcctgctg gtttctgtac ctgcctttgc ctggcagccg caaactggcg acatcatctt
      181 tcaaatctct cgctcatcgc aaagtaaagc gatccaactg gcaacccatt ccgattacag
      241 ccacaccggt atgctggtga tacgcaacaa aaagccctac gtttttgaag cggtcggccc
      301 ggtgaaatac accccgctca agcagtggat cgcccatggt gaaaagggca aatacgtcgt
      361 tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggcaaa
      421 acgttatctc ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg
      481 ttcggaagtg gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgaacagca
      541 gaagttgaaa gagtttgatc tcagcaaccc gctggtccag gcgaagctca aagaacgcta
      601 cggtaaaaat attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc
      661 acaactcacc acggttgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa
      721 aataattccc gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaagctgt
      781 cgctccggta aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta
      841 aggttgtttc atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta
      901 ccgtcacggg cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt
      961 atttcagctc agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga
     1021 aatgactttc aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa
     1081 tacagtcatc cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct
     1141 catgctgatg tggcactcat ctgcaaactt agtcagcaat ggagctttgt cggcagtaaa
     1201 gcgcgacagc actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac
     1261 acttttggcc cccggactga cgaaacctgt cgtgaattgc gggcgctgct taccctttta
     1321 ccatcggcat gttgaccact gacgaatggg gtagttacgc tagagagtta ccgaaggaga
     1381 agcatctgac tggtaagatt tttactcagc acattgagcg taacaatctg accctgagaa
     1441 cccgaatcaa acgtctggcc cgcagaacca tctgtttctc gcgttctgta gaacttcatg
     1501 aaaaagtcat cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt
     1561 ctgataagct ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca
     1621 ttctgctctt ttttctcaaa tgacatatat tatattttac atcatattca taggcatatg
     1681 acattccccc gccccccatt atgtcattgt gggttgatgt tgggctttca taaaaaaaac
     1741 atcttttttt gaactagcat taattatatt tactttatat attaaaatat tttctccatc
     1801 tggaaatatt ttagccacac aatataaagc agctgaatcc gaattactta aaaatatatt
     1861 atcatcaccc ataactccag ccattaaatg actggagact aaagcaagga aataacaaca
     1921 ttgtattgcc ttcattttaa ttactccctt gcctgataat tgtaactcca ttccctttag
     1981 ctttgggatt atattttaga taggtgttat ttggtgttcc caagtaacta ctgtattctt
     2041 ttttttccat tccattcata taagaattcg ttaaatcttt gcttgaaaaa tttagactat
     2101 cataaacatc attttctttt gttactttat ttcctttatc gtcagagtaa aaaccgtgag
     2161 ggtgataatc gcctactctc tctgaacctt cagggcattt tatatttcgc aagtctacac
     2221 tatcattatc atttgaactt atcggattca tgggtaaata ttcaccttgc ttcttacata
     2281 ttagtccacc atattcttta ttctcgttaa ttgattctcc attacacatt gccaatgcaa
     2341 atattccaat atcatctata ttttttgatt taaattcata taatcctaat ggatccatgc
     2401 tattcaccgg gctcagcgga tatgtataca g
//
LOCUS       DAHBHC010000237         2429 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHBHC010000237 DAHBHC010000000
VERSION     DAHBHC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.242"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            57..1592
                     /gene="istA"
                     /locus_tag="M2A35_005297"
     CDS             57..1592
                     /gene="istA"
                     /locus_tag="M2A35_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCB7201434.1"
                     /translation="METCFKILQLKFDQKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIYPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLKSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLAPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKAGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIGQGKRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRENLELPLSRQGEQHPAYASEHENLRGPG
                     YYH"
     gene            1609..2364
                     /gene="istB"
                     /locus_tag="M2A35_005298"
     CDS             1609..2364
                     /gene="istB"
                     /locus_tag="M2A35_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCB7201435.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNL
                     LITGPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNSHRVVLQGESLRKNPPVVESSEKTR"
BASE COUNT          648 a          647 c          657 g          477 t
ORIGIN      
        1 ccgtgtttaa accagtggag acggcaatgt ccagaaagag aaaatccagg agcactatgg
       61 aaacctgttt taagatcctt cagctcaagt tcgaccagaa gctgactaac cgttgtatcg
      121 gactgacgtt acacatcagc gcctcaaccg tttttgaggt tcttgcacgc ttcaaagcct
      181 cttctctgtc atggccatta cctgctgata tatcccatga cacgctggag aaactcattt
      241 acccgcccaa agacacctct gcgtcagagc ttgttatgcc tgacatgctg tacttcgata
      301 cggaaatgag aaaaccgggc gtgacgcgcc agctgttgtg gatggaatac aaggcacagg
      361 cgggtgacaa agcgatggga tactcgcact tctgccgttg ctaccgggag tggaaaaaaa
      421 cgcgacgtct ctccatgcgc caggagcatc gtgccggcga aaagcttttt atcgacttct
      481 gtggtcctac ggttcctgtt attaatccgg acaccggtga gatacgccgc gtggcaatct
      541 tcgtggccgt aatgggggcg tccaactata cctacgttga agcgtgcgaa ggtcaggaca
      601 tgatgtcatg gttaaatgcc cacagccgtt gcctgacctt cctgggcggc gtgccgaagc
      661 tgctcatccc cgataatctc aaaagtgcag tcaaaaaagc cgatcgctat gagcctgtca
      721 tcaacgacag ttatcaggcg ctggctgagc actatggcac ggttattatc ccggcccgcc
      781 cgcgcaaacc taaagataaa ccaaaagcag aaaacggcgt cctcatcgtc gaacgatggc
      841 ttcttgcccg catccgtaat gaaaccttcc acacgctcag ggcgctgaat gcgcgccttc
      901 gggagttact gacggacatg aataaccggc caatgaaggg gtacggcaat caaacccgtg
      961 cagaacgctt ccgcatgctg gatgcgccag cactctctcc gctgcctctg gcaccttatg
     1021 aatacacgga atacaaagcc gtgaaggttg gccctgacta tcacgttgaa tatgcccgtc
     1081 actggtactc cgttccacac gaactggtcg gtcagcgtct gtcactgaag gcggggcagt
     1141 ctgtggtaca actctggcac aaggggcagt gtgtggcgca acatccgcgc agcacacatg
     1201 aatataaaca cacaaccaat ccgcttcaca tgccagagcg ccaccggcgg cacgggacat
     1261 ggacaccgga acgccttatc gggcagggaa aacgtacagg cccgtccact ggccgggtgg
     1321 ttgaatccat gctgaaggca aaaccccatc cggaactggc ttaccgtgca gtcctggggc
     1381 tgctggctct tcagaaaaaa tacggcccgg aacggctgga aaaggcctgt tacgttgccc
     1441 tgcactataa cgcccctgac agacgcttta tcgacaatct gctgcgtcac catcgggaga
     1501 acctggagct tccattatcc cgccagggcg aacaacatcc ggcatatgct tcagagcatg
     1561 aaaacctgcg tggtccgggc tactaccact gacagaagga acaacagtat gagtgataac
     1621 ctgttaaaca aactgaccca actgaaactc ccggcgatgg ccgggagcct gatacggcag
     1681 cgggaaacgc ctcagaccta cgacgaactg tcgttcgagg aaaggctgac gctgctggtt
     1741 gatgacgagc ttctgagcag ggaaaacagc cgtgtggcac ggttgagaaa aaatgccggc
     1801 ctgaaatatc aggcaacccc ggaagggctg cgctatccgg cctcaagagg tctgcgggct
     1861 gaacagatga gggaactgct gaacggccac tacataaccc acaggaaaaa cctgctgata
     1921 accggtccga caggatgtgg caagagctgg atagccaatg cgctgggcga acaggcatgt
     1981 cgacagaaac acagcgtccg gtactgtaga accgggcgat tactggaaca actggctcag
     2041 ggacgtgttg atggcagctg gctgaaatat ctgaaacagt tgcaaaaaat acaggttcta
     2101 atcctggacg atctggggct ggaacagtta agtaacgccc agtgtaatga ccttctggag
     2161 ctcatagagg atcgttacgg acaaagcagc acgatagtgg taagccagtt cccggtagat
     2221 aaatggcatg gtctgatgga gaacccaaca acagcagatg ccatcctgga cagactggta
     2281 cataactcac accgggtggt ccttcaggga gagtcactga ggaaaaatcc cccggtagtg
     2341 gaaagcagcg aaaaaacgcg ttaaaaagcg gtagaaaaag ttaagttccc ggttgatcga
     2401 atggatgcga atcgctgatc ggacttcga
//
LOCUS       DAHBHC010000238         2429 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHBHC010000238 DAHBHC010000000
VERSION     DAHBHC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.243"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..70
                     /locus_tag="M2A35_005299"
     CDS             <1..70
                     /locus_tag="M2A35_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HCB7201436.1"
                     /translation="SELICAAECGEHIEPYKLGNIA"
     gene            67..180
                     /locus_tag="M2A35_005300"
     CDS             67..180
                     /locus_tag="M2A35_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201437.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSIKKIERFFTGE"
     gene            267..470
                     /locus_tag="M2A35_005301"
     CDS             267..470
                     /locus_tag="M2A35_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201438.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVRVNLIRQVQESRR"
     gene            467..712
                     /locus_tag="M2A35_005302"
     CDS             467..712
                     /locus_tag="M2A35_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCB7201439.1"
                     /translation="MTILDYIAANPGCSGGEIAAALNTPTTAINAELRRLWRSGSVIR
                     KERKTGGRFSYQINPMPFGCGNPLTNMFNQLLKEARA"
     gene            709..1008
                     /locus_tag="M2A35_005303"
     CDS             709..1008
                     /locus_tag="M2A35_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201440.1"
                     /translation="MSAINHQELRELATDLQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTPLAIRLTLRHEIEDFCATLEAPGEPETPEAIQKELLQRIDKVFDFFLNH"
     gene            1020..1637
                     /locus_tag="M2A35_005304"
     CDS             1020..1637
                     /locus_tag="M2A35_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCB7201441.1"
                     /translation="MHTQKNRLPCRNQSGYISAAPHKTGAGFRSLLTTQAHNRASGFF
                     VRTVLPRFFRVRIMAGRTGPTSVGPGSCVAGTANLVRLATHSFAALDGEFSQLTTQGA
                     TPWQTANHTALSRSVVTSRLKSTADFPAHHASRKSCTSICCMSAATHYQTFIPPLFSA
                     IWWMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSVTRGLPQ"
     gene            1634..1999
                     /locus_tag="M2A35_005305"
     CDS             1634..1999
                     /locus_tag="M2A35_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201442.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYVIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIAESIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            2006..>2429
                     /locus_tag="M2A35_005306"
     CDS             2006..>2429
                     /locus_tag="M2A35_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7201443.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYACGTLRK"
BASE COUNT          629 a          684 c          603 g          513 t
ORIGIN      
        1 ttccgagctg atctgtgccg cagaatgcgg ggaacatatt gaaccgtata agttggggaa
       61 tatcgcatga gtatatttat ctcatggctt gttctgatta tttcggtggc ctgcgctatt
      121 gggattatgc gaattattca ttcaataaaa aagattgaac gctttttcac tggcgaataa
      181 cagagcaaat aaaaccacag attaaataag aaaatgtaaa aacaatccgc attcgcggag
      241 gtattcgcac acgccaagga ggcgtaatgg caattaagca ttttcctgtc gttcgtttta
      301 cctccagagg gcgtgaatac gaagtcgacg aacgcctgat taccacaatc gacaaacacc
      361 gttcagaaaa ggatgcacat cacatctatc tcactgacgg cacttacttc tgcgccacga
      421 atgtggtgcg ggtgaacctt atccgacagg tacaggagtc acgcagatga ccattctgga
      481 ctatatcgcc gccaatccgg ggtgtagcgg tggagaaatc gccgcagcac tgaatacccc
      541 aaccacagcc attaatgcgg agttacgccg actctggcgc agcggttcag tcataagaaa
      601 agagcgcaaa acaggcggtc gcttttctta ccagataaac ccgatgccgt tcgggtgcgg
      661 caatccactt accaacatgt ttaaccagct actgaaggaa gccagagcat gagcgccatc
      721 aaccaccagg aattacgcga actggcgact gacctgcaac gaatggcaac gcctcaaaaa
      781 ttactggcgt ttcgcgcaat gctctcgccg tctgctgtgt tggcgctgct ggatgagctg
      841 gagcacgcca gaaccacacc tctcgccatt cgcttgacgc tccgtcatga aatcgaggat
      901 ttctgcgcga cgttggaggc accaggcgaa ccggaaacgc cggaagcaat acagaaagag
      961 ctgctgcaac gcattgacaa ggtttttgat ttttttctga accactaaga aaccagaaca
     1021 tgcacacaca aaaaaaccgc ttgccatgcc gcaatcagtc aggttacatt tccgctgcac
     1081 ctcacaaaac gggtgccggg tttcgcagcc tgctgactac acaagcgcac aaccgcgcca
     1141 gcggtttttt tgtgcgtact gtattgccac gtttttttcg cgtcagaatt atggcggggc
     1201 gtacggggcc gacttcggtc gggccgggtt cttgtgtagc cggtactgcg aacctcgtac
     1261 gtctcgccac ccacagtttc gcagctctgg atggtgagtt ttcacaactt actacacaag
     1321 gggccacacc atggcaaacc gcaaaccaca ccgcgctatc gcggagcgtc gtcacatcca
     1381 gactgaaatc aaccgcagac tttcccgcgc atcacgcgtc gcgcaaatca tgcacatcaa
     1441 tatgttgcat gagcgcagcc acgcactatc aaacatttat tccgcctctg ttttcagcta
     1501 tctggtggat gatctgcacg agcttcaaca gctcatccag cagcaaaaca aactccatta
     1561 attcctgttc cgggcctttc ctacaccttg cggcgggagg ccttcgcaca tctgtaacaa
     1621 gaggattgcc gcaatgattc tcgccaacga ctttcttgaa tacctgctca acacagaacg
     1681 tgatcttgcc gctcgcgtgc gtgatcgtta tgacatgtac ctgaaatccc tgcctgtacc
     1741 gcagctcgct gacggaaaaa ttgttattga tggtcgctac gtgatcgaca gccatgaggg
     1801 aaattacagg ctttaccgca ttgaaggtgg caccccgtcc gttattggca tttaccagcg
     1861 cccatcctct gcaatcgtcg atgtgattgc cgagagcatc cgcatcacac atcgccatgc
     1921 cgacacagaa gacaccgtgc tggaaattca gcggctggct acagtctgcc gcgacaccct
     1981 gaatggcatg acgaagtaaa tcactatgac ggcagagtac atcagggact ggcaacaacc
     2041 gcgccacgca gtggggcgtg aaggaacggg gatccccgct cctgaatccg cgctttcctc
     2101 ctggctggat gcctaccggg cagagaacga gcgccgccag gaaatggctg atgcggcgtt
     2161 ctccgccacg ccgctgggca acctgattaa taaaagcctg gacgcacagg aaaaacagga
     2221 caaaaccatc acactggcag gagacgccag aaaacaggca cgcggcgcgg tggatgaagc
     2281 catggcctcg ctgcgcctgc tgccgtccta tctgcgcgat ccgcttattc gccacctctc
     2341 cttcctgcgc aaaaaacagg aagccgatcg ccggaaaggc aaaaagagct ggcaggctga
     2401 acgctacgcg tgcggaaccc tgcgcaaaa
//
LOCUS       DAHBHC010000239         2422 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHBHC010000239 DAHBHC010000000
VERSION     DAHBHC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.244"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            369..509
                     /locus_tag="M2A35_005307"
     CDS             369..509
                     /locus_tag="M2A35_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201444.1"
                     /translation="MAENGYGLAGLGMGKVKSVNPYRLTPGFGGFTPVSYETTERRLP
                     CR"
     gene            615..861
                     /locus_tag="M2A35_005308"
                     /pseudo
     CDS             615..861
                     /locus_tag="M2A35_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1005..>2422)
                     /locus_tag="M2A35_005309"
     CDS             complement(1005..>2422)
                     /locus_tag="M2A35_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCB7201445.1"
                     /translation="AKRHASPEETYHAILNEGLKAFNLSLGIISQIEGDRYSLLAVSP
                     TRGDISAGMVFELKDTYCQRVVSENRIISVEHAGEHPGFNTHPVYIGMRLESYISAPI
                     WVRENLWGTLNFSSTQVRAISFSEDDYEFISLMAEGIGSLIEINLLISENENVISALR
                     KNNDILENIFENSTIGMALVAPSGQWVKVNSALTRMLEYTEDYLLSINFQHITHPDDL
                     TTDLQQLEALSQGNIPFYQLEKRYLTASGHYIWILLSVSLIREDNGNVKYYIAQIQSI
                     DERKMMEIELKKQKEALHKVNIILERMATEDSLTEIANRRKFMLWFEREMIRMARHLV
                     PVSLAIADIDFFKSYNDDYGHQEGDFSLKSIARELSHTLRSQDKVARFGGEEFIMLFP
                     ETDEKGCLLACERLRKSVENLTSLRRTVTISIGAVTYHPKEGELVHFDDLLKVADSKL
                     YEAKRSGRNQVKVTNLEDHQD"
BASE COUNT          634 a          587 c          539 g          662 t
ORIGIN      
        1 ttcattgcct gtcctgtgga cagacagata tgcacctccc accgcaagcg gcgggcccca
       61 accggagcca ttttagttac aacactcaaa tacaaccccg ggaaaacccc ggtccagcgc
      121 agaaccgaaa ccacaaagcc cctccctcat aactgaaaag cggccccgcc ccggcccaaa
      181 gggccggaac agagtcgctt ttaatcatga atgttgtaac taataatcat catcgctgtc
      241 agtcttctgg ctggaaattc tcagtacacg ctcgtaagcg gccctgacgg cccgctaact
      301 cggagatacg ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa
      361 gctctgtcat ggctgaaaac gggtatggtc tggcagggct ggggatgggt aaggtgaaat
      421 ctgtcaatcc gtaccggctt acgccgggct tcggcggttt tactccggta tcatatgaaa
      481 caacagagcg ccgccttcca tgccgctgac gcggcatatt ttccctttct tttgtcagac
      541 atgacaacat cagcacagca taataataat caataaactg tgtcacgttt acatcatgga
      601 acggaggtac tcctgtgacc tttgagcaga aaaaagcccg ggccattgcc ctgatggaca
      661 gcaaaaaaat gtggcggagc aattatgccc ctcctcttct gcgtattctc tggcggctgg
      721 gtatccggct ccgccattac cctttatgcc gttctggcag gtcacggtgc tgacgggggc
      781 ttatggggca tttcctaggg atgtgcaatg tggtttatct actgggggcc ctcaggaatg
      841 gtagcaggtg aggctattat taggggtttg agggccaatg atgtaaaaaa ccacgctaag
      901 gagataatta attgtttgta ttgaaattta atggtgcaga aaactgtcat gcccgacagc
      961 cacctaaagg tttttaccta attcgaatta agatagaaaa gagattagtc ctgatggtct
     1021 tctaagttgg tcactttcac ctgatttcgc ccagaccttt tcgcttcata gagttttgag
     1081 tctgccactt tcagtaaatc atcaaagtga acaagctccc cttctttcgg atggtatgtc
     1141 actgcaccaa tactgatcgt gaccgttctt ctgagcgagg tcaggttttc aacgcttttt
     1201 cgtaatcttt cgcaagccaa aagacagccc ttttcatccg tttcagggaa aagcatgata
     1261 aattcctccc cgccaaaccg tgcgacttta tcctggcttc gtagagtatg actgagttct
     1321 ctggcgatac tctttaggga aaaatcgcct tcctggtgac cataatcatc gttatacgat
     1381 ttaaaaaaat cgatatcggc aatggcaagt gatacaggga cgagatggcg tgccattctg
     1441 atcatttcac gctcaaacca gagcatgaat tttcgacgat tggcaatttc cgtcagactg
     1501 tcttccgtgg ccatgcgctc cagaataata ttgaccttat gcagcgcctc tttttgtttt
     1561 ttgagctcta tctccatcat tttacgctca tcaatacttt gaatctgagc gatgtaatat
     1621 ttgacattac cgttatcttc acgtatgaga gagacactca gaaggatcca gatataatgg
     1681 cctgacgccg tcagatatcg tttttccaat tgatagaacg gaatattgcc ttgggaaaga
     1741 gcctctaatt gctgtaagtc cgtggttaaa tcatcaggat gtgtgatgtg ctgaaaattg
     1801 atggaaagca aataatcttc agtgtactcc aacatacggg tcaaggcgct gttaaccttt
     1861 acccactggc ctgaaggggc taccagcgcc atcccgatgg ttgagttttc aaaaatgttt
     1921 tcgagaatgt cgttattttt tcttagcgct gaaatgacgt tttcattttc actgattaac
     1981 aagttaatct cgattaacga gccaataccc tcagccatta aactaatgaa ttcatagtca
     2041 tcctcagaga aagatattgc tcttacctga gtggaagaaa aattaagtgt gccccataaa
     2101 ttttctctca cccatattgg tgccgagata taactttcaa gccgcatgcc aatgtagacg
     2161 gggtgagtat taaaaccagg gtgctctcct gcatgttcaa cgcttattat tctattttcg
     2221 ctgaccactc gctggcaata ggtgtctttt aactcaaaaa ccatgccagc tgaaatatct
     2281 cctcttgtgg gtgaaactgc aagcagagag taacgatctc cctctatctg actgataata
     2341 cccagggaca ggttaaaggc ttttaaccct tcattgagga tagcatggta ggtctcttca
     2401 ggagaagcat gcctcttggc ca
//
LOCUS       DAHBHC010000240         2375 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHBHC010000240 DAHBHC010000000
VERSION     DAHBHC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.245"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..273
                     /locus_tag="M2A35_005310"
     CDS             <1..273
                     /locus_tag="M2A35_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HCB7201446.1"
                     /translation="AKYTIIDWVAFLQVLLIWFYMAYRSGQWIVSVACSKGWRWWNRK
                     NKKALALDSFYEAFNLNSLQPGSVVVVTTQSGMTIQIHKPKEEGRG"
     gene            266..550
                     /locus_tag="M2A35_005311"
     CDS             266..550
                     /locus_tag="M2A35_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCB7201447.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYGGYEIKTITHPHFGDKPVKVYAIKVNIGNE"
     gene            600..1331
                     /locus_tag="M2A35_005312"
     CDS             600..1331
                     /locus_tag="M2A35_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201448.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEVRKLCSEKPVRNNDFIA
                     KVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDKLESMQQQ
                     LQQKTTTKKSPDGLEEFRKARALKMTVDTMKDLFDFLPHLAPEAKQVVAASLINPVVG
                     FSAIPLPVIDEHHYSASEVGTMLGISANKVGRIANTYMLKTEKYGKWFIDKSAHSDKQ
                     VETFRYNEAGVHKIEELIEGERKAA"
     gene            1653..2276
                     /locus_tag="M2A35_005313"
     CDS             1653..2276
                     /locus_tag="M2A35_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCB7201449.1"
                     /translation="MTSQLIPVFNGTIANETALLCNARDLHAFLGVKKVFAAWITNRI
                     SEYEFIENQDYILLSNLGKQTSGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDISKRLYPIMKELNSSYASKLYD
                     IAFETFYAVTKNRDVLLREATRLDQTSAVFERARPMLKSLRARQFEF"
BASE COUNT          717 a          554 c          568 g          536 t
ORIGIN      
        1 gcgaaatata cgattattga ctgggtagcc ttccttcagg ttttgctcat ctggttttat
       61 atggcttaca ggagtggaca gtggattgtc agtgtagcct gtagcaaggg atggcgttgg
      121 tggaaccgaa agaataaaaa agcgctggcc ttggattcgt tttacgaagc attcaatctt
      181 aacagccttc agcctggttc tgtcgttgta gtcaccactc aaagcggcat gacgatacaa
      241 attcacaagc caaaggagga aggtcgtggc taacctgcaa cttgccgtta aaggtgaata
      301 cttcgatgcc atgattcgag gagagaaaac ggaagagtat cgcctgtgta atgactactg
      361 gaataagcga attatgttcc gggagtatga ccgcctgatt atcacaaagg gatatcctaa
      421 gcgtgacgat tccagccgca gaattgacgt tccgtatggc ggatatgaaa tcaagacaat
      481 cacacacccg cacttcggcg ataaaccggt aaaggtttac gcgataaagg tgaatatcgg
      541 caatgaataa caatcctcgc attggcgggg atttctttta tctgaaaagg acagaataca
      601 tgattaacaa tattgcaaat cagtcttcgt ttccggctgc aacaattgac agccagatgc
      661 tgttgaaaat ggttaatgag gtgcggaaat tatgtagcga aaagcccgtt cgcaataatg
      721 atttcattgc caaagttaag gatgagcttg aaggagagac ctacgaaatt ttcgtaggtc
      781 agaaaaacgg cgcagaaata gaagttataa caatgacttt caagcaagcc ctccgcgtcg
      841 ccgcacgtga gtcgaaagcc gtccgtcgtt cgctggtcga caaacttgaa tcaatgcaac
      901 agcagttgca acaaaaaacg accacgaaga aatcaccgga tggccttgaa gaattccgta
      961 aggcacgcgc attgaaaatg accgtcgaca caatgaaaga cctcttcgac ttcctccctc
     1021 acctcgcacc ggaagctaaa caggtcgttg ccgccagcct gattaatccc gtcgtcggct
     1081 ttagtgcaat ccctcttccg gtcattgatg aacatcacta ctctgcgtcg gaggttggca
     1141 ccatgcttgg catttcagcc aacaaggtag ggcgcattgc caacacctac atgctcaaaa
     1201 cagaaaagta cgggaagtgg ttcatcgaca aatcggcgca cagtgacaag caggtcgaga
     1261 catttcgcta caacgaggcg ggagtgcaca agatcgagga actcatcgaa ggagaacgaa
     1321 aggctgcatg attttgacaa gatagttttc cccaaatatg gggaaaagcc cgaatggcgc
     1381 ggcttacagc aagataaaga ccacatgatt tgacaaatcc gcattaacgg ggctatattc
     1441 cacttcatgg tgctgaacac accttgcaaa gcggaaaccg cacccgtcag tcatgcggct
     1501 tttttatgtc catttctcag atatggtcgg gtagcgcgta taccgaaaaa caaccgaaag
     1561 gttaaggata cgggccgact ttgcacggtg ttcaagtacc tgaccgccct gctgaacaca
     1621 gggctatctg aacaaatgca aaggacataa aaatgaccag tcaactcatc cccgtattca
     1681 acggcactat cgccaacgaa acagccctac tctgcaatgc ccgcgatctg catgcttttt
     1741 taggcgtgaa aaaggtgttt gcggcatgga ttacaaatcg catttcagaa tacgaattca
     1801 ttgaaaatca agactatatt ttgctttcca atttgggaaa gcaaacatct ggtagaggcg
     1861 gtcacaaccg caaggattac cacctcaccc tcgacacagc caaagagctg gcgatggtgg
     1921 agcgcaacga aaaaggccgc cagatacgcc gatacttcat cgagtgcgag aaaaaacttc
     1981 gcagcatgca accagcgcaa caattcacag acgaggaaat catcctcctc tgctacatgc
     2041 aggtacagat ggagaatgca caggacatca gtaaacgcct ttacccgata atgaaggaac
     2101 ttaactcatc atacgcgagc aagctgtatg acattgcgtt tgaaaccttc tatgcggtga
     2161 cgaaaaacag agatgtactg ctcagggagg cgacacgact tgaccagaca agcgccgttt
     2221 tcgaacgggc aaggccaatg ttgaaaagcc ttcgggcgag acaattcgaa ttttaatcat
     2281 caaaggagct tcggctcctt ttttgttggg gaaaaatcaa ccactactcg ttcccttgcg
     2341 agtaattgcg gagactttgc gatgtacttg acact
//
LOCUS       DAHBHC010000241         2374 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHBHC010000241 DAHBHC010000000
VERSION     DAHBHC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.246"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(190..435)
                     /locus_tag="M2A35_005314"
     CDS             complement(190..435)
                     /locus_tag="M2A35_005314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCB7201450.1"
                     /translation="MMKYLLLAVSLFTSGYSMAHTPHEIQRNNYISATGTTPDNVVNN
                     LYKKAEKRGERIVKITSVGGNNILYGSAITVKDGVDI"
     gene            complement(858..1190)
                     /locus_tag="M2A35_005315"
                     /pseudo
     CDS             complement(858..1190)
                     /locus_tag="M2A35_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1256..>2374
                     /locus_tag="M2A35_005316"
     CDS             join(1256..1517,1517..>2374)
                     /locus_tag="M2A35_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCB7201451.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIACLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWC"
BASE COUNT          645 a          583 c          521 g          625 t
ORIGIN      
        1 catcagtcac tgacaccatc aaagcattag ctgtgcagca gtgtgtaaat agaaagataa
       61 aatatctgaa aattatgtca ttatttaaat cagcgttagc ttaacagaat ggataaaagg
      121 attgcatata tttattaatt tttttatggg aaggttacaa agatatatgc gtaaccttcc
      181 atgcatatct tatatatcta ctccatcttt tactgtaatt gctgaaccat atagaatatt
      241 gttacctcca acagatgtta ttttcacaat acgctcacct cttttttcag cttttttata
      301 tagattgtta acaacattgt caggagttgt gcctgttgca gaaatataat tgtttctctg
      361 tatctcatgc ggagtatgag ccattgaata gcctgaagta aacaaagaga cagcaagtaa
      421 tagatatttc atcatatttc cctcattcaa aatagccttt tcttggtatt aagaatttaa
      481 atgaaaattt atttgcgttt tgcgatcaac caccactaac tgatatcttg atattaattg
      541 ttattatttc tccatttctg ttttatatac ctcttggctt attgttttca tttgaactga
      601 tacttggaat gcattataac accctatttg aaaataaacc ttgcaacatg attgcatttt
      661 tgtaatgttc atcgatagga attaaaaccc ccaaaggtta aaaaaacacc acaaaacaga
      721 tgtttcttca acaccacttt tactccatat gaaaggaatc gacgattaaa ctggatggct
      781 ctgattgatt cagggtatga atggcggttt tttgctccgt ttccctcaaa atggacgcaa
      841 cttcccctct gcggctctca gccgcaacac tgcattcggg ccagcggctc atgcatcata
      901 accagctctg ccagacggta tccccgcttc agccccgtaa accgcatctg acttccgcac
      961 aggacgcatt tcagcgggtc aaccttcagt aaccgctgat acatccctct ccacctgatt
     1021 tgcatcgccg tttttctcac cgtctccgtt atgacgtaca ccacctcttc cagtaaccgc
     1081 cgtttcgccg gactcaaaaa accgtagtac ctcaccatac ggaacccctt atccgccaca
     1141 tgccacgaga acctttccat gaactcatct ccactcatca ccaggtattc tgatcttacc
     1201 cagcaatagt ggacacgcgg ctaagtgagt aaactctcag tcagaggtga ctcacatgac
     1261 aaaaacagta tcaaccagta aaaaaccccg taaacagcat tcgcctgaat ttcgcagtga
     1321 agccctgaag cttgctgaac gcatcggtgt tactgccgca gcccgtgaac tcagcctgta
     1381 tgaatcacag ctctacaact ggcgcagtaa acagcaaaat cagcagacgt cttctgaacg
     1441 tgaactggag atgtctaccg agattgcatg tctcaaacgc cagctggcag aacgggatga
     1501 agagctggct atcctccaaa aggccgcgac atacttcgcg aagcgcctga aatgaagtat
     1561 gtctttattg aaaaacatca ggctgagttc agcatcaaag caatgtgccg cgtgctccgg
     1621 gtggcccgca gcggctggta tacgtggtgt cagcggcgga caaggataag cacgcgtcag
     1681 cagttccgcc aacactgcga cagcgttgtc ctcgcggctt ttacccggtc aaaacagcgt
     1741 tacggtgccc cacgcctgac ggatgaactg cgtgctcagg gttacccctt taacgtaaaa
     1801 accgtggcgg caagcctgcg ccgtcaggga ctgagggcaa aggcctcccg gaagttcagc
     1861 ccggtcagct accgcgcaca cggcctgcct gtgtcagaaa atctgttgga gcaggatttt
     1921 tacgccagtg gcccgaacca gaagtgggca ggagacatca cgtacttacg tacagatgaa
     1981 ggctggctgt atctggcagt ggtcattgac ctgtggtcac gtgccgttat tggctggtca
     2041 atgtcgccac gcatgacggc gcaactggcc tgcgatgccc tgcagatggc gctgtggcgg
     2101 cgtaagaggc cccggaacgt tatcgttcac acggaccgtg gaggccagta ctgttcagca
     2161 gattatcagg cgcaactgaa gcggcataat ctgcgtggaa gtatgagcgc aaaaggttgc
     2221 tgctacgata atgcctgcgt ggaaagcttc tttcattcgc tgaaagtgga atgtatccat
     2281 ggagaacact ttatcagccg ggaaataatg cgggcaacgg tgtttaatta tatcgaatgt
     2341 gattacaatc ggtggcggcg gcacagttgg tgtg
//
LOCUS       DAHBHC010000242         2366 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHBHC010000242 DAHBHC010000000
VERSION     DAHBHC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.247"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..302
                     /locus_tag="M2A35_005317"
     CDS             <1..302
                     /locus_tag="M2A35_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCB7201452.1"
                     /translation="LQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVS
                     IRISLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLRMR"
     gene            440..1459
                     /locus_tag="M2A35_005318"
     CDS             440..1459
                     /locus_tag="M2A35_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCB7201453.1"
                     /translation="MKYTPVGVDIAKHLIQVHFINEHTGEVVDKQLRSRDFLTFFSNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRRQLVKFRTAQINSLHGTLLEFGESIHKGRAAMGREF
                     PEALERMKERLPQYLITVLENQYNRLSELDSLIDDIEKQLTSVARQNETCKRLLGIPG
                     IGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGDTYLRTL
                     FIHGARAAALLTREPSPWITELKKRRPASVAIVAMANKLARTVWAIAAYGRKYDKNHV
                     SIRPY"
     gene            1751..>2366
                     /gene="istB"
                     /locus_tag="M2A35_005319"
     CDS             1751..>2366
                     /gene="istB"
                     /locus_tag="M2A35_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCB7201454.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWG"
BASE COUNT          633 a          561 c          686 g          486 t
ORIGIN      
        1 atctgcagcc gttaccggat acggacttcg ataccagtta cttcgacatc cgccatgtgt
       61 cctgggacag ctatatcgag gttggcggta atcgttacag cgttcccgaa gcgctgtgtg
      121 gtcagccggt atcgatacga atatcgctgg atgacgagtt gcggatctac agtaatgaga
      181 aactggtggc ctcacatcgc ctctgttcgg catcgtctgg ctggcagaca gtgccggagc
      241 atcacgcccc gctctggcag caggtcagtc aggtggaaca tcggccactg agaatgagat
      301 gaacgcctcc caccattaaa tccggagcac aaaggtagat ggcgggaaaa tacaggaagg
      361 tgtggagcct cgcagatttc cggcatcata gtgcccaata cgggatgcat aagactccct
      421 cagactggag actccacaaa tgaaatatac accggttggc gttgatatcg caaaacacct
      481 gattcaggtt cacttcatca atgagcacac aggtgaagtg gtggataagc aactacgcag
      541 cagagatttt ctgacgttct ttagtaaccg cgaaccctgc ctgattggca tggaagcctg
      601 tggaggctct cagcactggg cccgggaact gacaaaactg ggccataaag tccggttgtt
      661 gcaggcccgc tttgttaagg catttgtcat gggcaataag aatgatgtga tggatgccag
      721 ggctatctgg atggcagttc agcagccagg taaagaaatc gccgttaaaa cagaagaaca
      781 gcagtcagtt cttgttctgc accggacccg cagacaactt gtgaagtttc gcacggcaca
      841 gattaactct ctgcacggca cgctcctgga gtttggtgaa agcattcaca aaggccgggc
      901 agcgatgggg cgggagttcc ctgaagcact ggagcggatg aaagagagac tgccacagta
      961 tctcattacg gttctggaaa accagtacaa ccgtctgagt gagctggact cactgataga
     1021 tgatatcgaa aaacagctta ccagtgtggc caggcagaat gaaacctgta agcggttgct
     1081 gggtattccc ggcattggac cacttatcgc gacggcagcg gtggccacca tgggggaagc
     1141 atctgcattt aaatcagggc gagagttcgc ggcttatgtt ggtctggtgc ccaaacagac
     1201 tggctcagga ggaaaaatcc gcctgctggg gataagtaaa cgtggtgata cctacctccg
     1261 gacattgttt atccacggag caagggcggc agcattactg accagagaac caagcccgtg
     1321 gataaccgaa ctgaaaaaac gtcgtccggc cagtgtggca atcgtcgcca tggcaaataa
     1381 gctggcacga acagtgtggg caatagctgc ttacggacgt aaatacgaca agaatcacgt
     1441 cagtatcaga ccatattaat tgctgagact gataaacatc aaaccttaaa aagggtgaat
     1501 gctgaaaggt tgctatggcg gccgatgtga tgacaacgac aggtaagacc gtgacctgat
     1561 aaacctgaat aggcccatgg acatcaagtc cgcactgaga ataaggctca ggtcagcgaa
     1621 tctcatagtg gcccgcagct ctggctgcaa taaggccgga tacagagctg caactactcg
     1681 tcgatgtcag aacaacggaa gccttgcaaa cggggaggcg ttcatacagt gcctatgagg
     1741 agctgttgtg atgcatgagc tggaagtcct gctgagtcgc ctgaaaatgg agcatctgag
     1801 ttatcacgtt gaaagcctgc tggaacaggc agctaaaaaa gagctgaact accgggagtt
     1861 cctgtgcatg gcgctacagc aggaatggaa cggcaggcat cagcgcggta tggagtccag
     1921 gctgaagcag gctcgtctgc cgtgggtcaa aacgctggag cagttcgact ttaccttcca
     1981 gccgggcatc gaccgtaagg ttgtccggga actggctggt ctggcgttcg tggagcgcag
     2041 cgaaaacgtg atcctgctgg gacctcctgg tgtaggaaaa acacatctgg ccatagctct
     2101 tggcgtgaaa gcggtggatg cgggacatcg ggtactgttt atgccactgg acagactgat
     2161 cgcgacactg atgaaagcga aacaggaaaa ccggctggag cgtcagctac agcaactgag
     2221 ttatgcccgg gtgttgatcc tggatgaaat aggctatctg ccgatgaaca gagaggaagc
     2281 cagcctgttc ttccggctac tgaaccgtcg atatgaaaaa gcgagcatca tactgacgtc
     2341 aaacaagggg ttcgcagact ggggag
//
LOCUS       DAHBHC010000243         2349 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHBHC010000243 DAHBHC010000000
VERSION     DAHBHC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.248"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..534
                     /locus_tag="M2A35_005320"
     CDS             <1..534
                     /locus_tag="M2A35_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201455.1"
                     /translation="PDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVS
                     GVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVI
                     GKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIK
                     ADNPIKAKQGSSDASNY"
     gene            518..1000
                     /locus_tag="M2A35_005321"
     CDS             518..1000
                     /locus_tag="M2A35_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201456.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNIIYKIINDQKISRGNGHFISIMVNNYRTHCGVVDINLNFFNDILYSVRLK
                     NISKLENMEFCAKKQRVYFSDKNKKASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS
                     "
     gene            complement(915..1100)
                     /locus_tag="M2A35_005322"
     CDS             complement(915..1100)
                     /locus_tag="M2A35_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201457.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            1181..2307
                     /locus_tag="M2A35_005323"
                     /pseudo
     CDS             1181..2307
                     /locus_tag="M2A35_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          832 a          322 c          530 g          665 t
ORIGIN      
        1 ccggacccga ttgggttgag aggtggatgg aatatgtatc agtatccgtt gaatcccata
       61 caagtgatag acccaatggg gttagatgcg attgagaata tgacatcagg tggactaatt
      121 tatgccgtat ctggtgtacc tggattgatt gctgcaaaca gcattactaa cagtgcttac
      181 cagttcggtt atgatatgga tgctattgtt ggcggagctc ataatggggc cgccgatgca
      241 atgagacatt gttacttgat gtgtcgaatg actaagacat ttggatcaac aatagctgac
      301 gtgataggta aaaatcatga ggcggcaggg gatagacaag gtcagccagc taaagaaaga
      361 atcatggatc ttaaaaataa cactgtcggt attgcttgtg gcgatttttc tgccaaatgt
      421 agcgatgcat gtattgaaaa atataacact gggcaactct tcgggttaga tggtataaaa
      481 gcagataatc caataaaagc aaagcaaggg agttcagatg cttcaaatta ttagaggcaa
      541 acttgtcatt tttttaatta ccctttgttt atttgttgtt taccttgggt ttgataacaa
      601 ttcaaattct gacatcgtat tttatggaca taaaacacca aagagcgttg agatatatct
      661 ttctgaaaaa aatattattt ataaaataat taatgaccaa aaaattagta gagggaatgg
      721 tcattttata agtataatgg ttaataatta caggacgcac tgtggagtgg ttgatataaa
      781 tcttaatttt ttcaatgata ttctttatag cgtgcgatta aaaaacatta gtaaactgga
      841 gaatatggag ttctgcgcta aaaaacaacg ggtgtatttt agtgataaaa acaagaaggc
      901 tagttataaa ataattaatt atggagacta ttacgatgtt gattattatg ataacaattt
      961 gaaaaatgaa gtttttgact ggattggtaa atggtcataa tgaacaacac cagaaagaga
     1021 aaaatataaa atacatacag aaagacatga gagcaaggga cggggcaggg caagatcatg
     1081 aaaattgtga tgtaaatcac gattttcatc ttggctttaa cgcctacagg tgatcaaaat
     1141 ttaacctttg gaataactaa aaagataaaa aaggacccag gtgagtattc aaagtttgct
     1201 tgattatatt tcagtgatcc ctgatatacg acaacaagga aaggttaaac ataaattatc
     1261 tgctattttg tttctcaccg tatgtgcagt aattgcaggt gccgatgagt ggcaggaaat
     1321 tgaagatttt ggacatgaaa gacttgaatg gctaaagaaa tatggtgatt ttgataatgg
     1381 cattccggtc gatgacacca ttgcacgcgt tgtgagtaac attgacagtt tggcctttga
     1441 aaagatgttt attgaatgga tgcaggagtg ccatgaaatc actgatggtg aaattatagc
     1501 aatagatgga aagaccataa gaggctcctt tgataaggga aaaagaaaag gagcaatcca
     1561 tatggtgagt gcattctcga acgaaaatgg tgttgtactg gggcaggtga aaacggaagc
     1621 caaaagtaat gagattacag ccattccaga gttgcttaac ctactgtatt taaagaaaaa
     1681 tttgataacc attgatgcta tgggctgtca gaaagatatc gcttcgaaga tcaaagataa
     1741 aaaagcagat tatcttctgg cagtaaaagg caatcagggg aaattacatc atgcattcga
     1801 ggaaaaattt cctgtaaatg tgttttctaa ttataaaggc gattcgttta gtacgcagga
     1861 gataagtcat ggaagaaaag aaacacgttt gcatattgtc agtaacgtaa cgcctgaact
     1921 tttgtgattt tgaattcgaa tggaagggat taaaaaagct ttgtgtagca ttgtcattca
     1981 ggcagaagaa agaagataaa tcagcagaag gtgtaagcat ccgatattat atttcatcaa
     2041 aggatatgga tgctaaagaa tttgcacatg ctatcagagc gcactggctg atcgagcaca
     2101 gtcttcattg ggtgttagat gtaaaaatga atgaagatgc cagccggata agaagaggaa
     2161 acgcagccta aataatatct ggaataaaga agatggcact gaatttatta agagattgca
     2221 aagacattaa gggtgaagag gaaaagaaag aaggctgcgt taaacacagg gaaagatcat
     2281 cagaagttca ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc
     2341 tcattacct
//
LOCUS       DAHBHC010000244         2340 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHBHC010000244 DAHBHC010000000
VERSION     DAHBHC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.249"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(35..226)
                     /locus_tag="M2A35_005324"
     CDS             complement(35..226)
                     /locus_tag="M2A35_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HCB7201458.1"
                     /translation="MSTLKRDKSPRGEGWSPTFQIRISKELRQQVNEAATADGLTLGN
                     WFKELARAELKRRGIEPKG"
     gene            225..569
                     /locus_tag="M2A35_005325"
     CDS             225..569
                     /locus_tag="M2A35_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCB7201459.1"
                     /translation="MYVRNSYYHQYYVRTLTVSATKNKAPQVSLPLAGLLTTNDNESI
                     EVAMRNHTIHPQGRDSHNLNKFAWRFIALSAAKPRVITIVATSEQEARQQSPAGCVMV
                     FTARIRQGEYYA"
     gene            562..819
                     /locus_tag="M2A35_005326"
     CDS             562..819
                     /locus_tag="M2A35_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201460.1"
                     /translation="MRDERFILLEQKFSEAPKNEIDALLHIANMLKVATFLIVSNLEH
                     ETALDILNSAVDYSEYIAEDKYRQLPDLLAHKYKEEPHTGK"
     gene            822..1124
                     /locus_tag="M2A35_005327"
     CDS             822..1124
                     /locus_tag="M2A35_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCB7201461.1"
                     /translation="MKKIKFTGLNASGQTHPKIRIGDIFRDKYGDSVVINSVYERHIT
                     YRRAGYDYDCVMPVHQFRRDFSLVCAVPRSKPTSKEKAQANIQVIKNMINAFRGKK"
     gene            1121..>2340
                     /locus_tag="M2A35_005328"
     CDS             1121..>2340
                     /locus_tag="M2A35_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCB7201462.1"
                     /translation="MKRAPNLKHQPKDKMTEVIIFAGSDAWAHAKEWSEWAGKHIAAD
                     DTHPVILGPEQLASLADTQIIDKGRYYVRVYRAGEISEQHLTQIATLLAVAGVKEARC
                     YRSFVDQQPEDWTPRMAGLKDDAEHGKSLVINLPAKTNFTGNYDDELKPRVESRVDGV
                     FWVTPKVDKQSGEIIRPETWLCSPLELLGTGTIGKEHYRVMRWKKPANHEVITMAVPC
                     GGIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGSHEEWQLSTTTGWHFGAYIMP
                     DGSVIGESEKPILFTGKTAAVNGYSVAGTAEGWRDTVARLAGGNPSMMLGVAVSLSAP
                     LIGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHN
                     DGLLPLDEIGQAGNA"
BASE COUNT          643 a          533 c          629 g          535 t
ORIGIN      
        1 aaagtttgtt atcggtctgc ttttcagtca tacatcagcc tttaggttca atgccgcgtc
       61 gctttagttc ggcacgagct aattctttaa accaattgcc gagggttagg ccatcggcgg
      121 ttgctgcctc gtttacttgc tggcgtagtt ctttgctaat tcgtatctgg aatgtcggcg
      181 accatccctc gccgcgtggt gatttatccc gctttaatgt tgacatgtac gtacgtaact
      241 cctattatca tcaatattac gtacgtacat taacagtaag tgcaacaaaa aacaaagccc
      301 cgcaagtgtc gttaccactc gcagggcttc taaccaccaa cgataacgag agtatcgagg
      361 tagctatgag aaatcatacc atacacccgc aagggcggga ttcgcacaac ctgaataaat
      421 tcgcctggcg ttttatcgcc ctgagcgccg caaaaccgcg tgtgattacc atcgtggcca
      481 ccagcgaaca ggaagcacgc cagcaatccc cggctggctg cgtgatggta tttactgccc
      541 gcatccgtca gggggaatac tatgcgtgat gaacgattta ttcttctgga gcaaaaattc
      601 agtgaagcgc caaaaaatga aattgatgct cttttgcata tcgcgaatat gctaaaagta
      661 gcaacgttcc ttatagtttc gaatctggag catgaaacgg cgttagatat actgaacagt
      721 gctgtcgatt attctgaata tattgccgag gataaataca ggcaattacc agatttgtta
      781 gcccataaat ataaagaaga acctcacacc gggaaataaa catgaagaag attaaattta
      841 ctggcttaaa tgccagcgga cagactcacc cgaaaatcag gatcggggat attttccggg
      901 ataaatatgg tgacagcgtg gttattaatt ccgtgtatga acggcacatt acataccgcc
      961 gcgcagggta cgactatgac tgtgtgatgc ccgttcatca gttccggcgt gatttttcac
     1021 tggtatgtgc agtaccacgc agtaagccca ccagtaaaga gaaagcacag gcaaatattc
     1081 aggtaataaa gaacatgatt aacgcattca ggggcaagaa atgaaacgcg caccgaattt
     1141 aaagcaccag ccgaaagaca aaatgacgga ggtgattatc tttgcgggta gtgatgcctg
     1201 ggcacatgcg aaagagtgga gcgaatgggc cggaaagcat attgcggcag atgatacaca
     1261 tccagttatt cttggaccgg aacaactggc gagtctggca gacacgcaaa tcatcgacaa
     1321 agggcgctat tacgtgcgcg tttatcgtgc tggtgaaata tcagagcagc accttacaca
     1381 gatagcgaca ctgcttgcag tggcgggggt gaaggaggcg cgttgctacc gcagctttgt
     1441 tgatcagcag ccggaggact ggacaccgcg catggctgga ctgaaggatg atgcggaaca
     1501 cggtaaaagt ctggtgatca accttccggc gaagacaaat ttcaccggaa attatgatga
     1561 cgaattaaag cctcgtgttg aaagtcgcgt cgatggtgtt ttctgggtaa cgcccaaagt
     1621 ggacaagcag tcaggcgaaa ttatccggcc tgagacgtgg ttatgttctc cgcttgaact
     1681 actgggaaca ggaacaatcg gaaaagagca ttaccgcgta atgcgctgga aaaaaccagc
     1741 aaaccatgag gtcattacaa tggcggtccc gtgcggaggc attggtgacc gtgacggctg
     1801 gcggttgctg aaagaccacg gtcttaacgt gacgaccaac ggtaaataca gagccatcct
     1861 ggctgactgg atgcagttaa gcggaagtca tgaggaatgg cagctaagca ctacaacggg
     1921 ctggcatttt ggtgcgtaca tcatgccgga tggttcggtc attggtgaat cagagaaacc
     1981 catcctgttt accggaaaaa ctgccgctgt taatggctat tccgtggcgg gaacagcgga
     2041 gggctggcgc gataccgtgg cacggctggc tggtggtaat ccgtccatga tgctgggtgt
     2101 ggcggtatcg ctatccgcac cattaatcgg gctggtgggt gctgacggct tcggggttca
     2161 tctttttgag caatcatcag caggtaaaac caccacacag aacattgcat caagtttatg
     2221 gggagagccg gacgcgcaac ggctgacctg gtacggaacg gcgttaggta tcgccaacga
     2281 ggccgaagca cataatgacg ggctgttacc cctggatgaa ataggccagg ccggaaacgc
//
LOCUS       DAHBHC010000245         2335 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHBHC010000245 DAHBHC010000000
VERSION     DAHBHC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.250"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..778)
                     /gene="cif"
                     /locus_tag="M2A35_005329"
     CDS             complement(<1..778)
                     /gene="cif"
                     /locus_tag="M2A35_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HCB7201463.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFS"
     gene            complement(1002..1883)
                     /locus_tag="M2A35_005330"
     CDS             complement(1002..1883)
                     /locus_tag="M2A35_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HCB7201464.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTASQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(2047..2178)
                     /locus_tag="M2A35_005331"
     CDS             complement(2047..2178)
                     /locus_tag="M2A35_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201465.1"
                     /translation="MVFIFRMLQIATDEETSLLTVWKRYRVLLNRVDTSVSPDFEGQ"
     gene            complement(2204..2335)
                     /locus_tag="M2A35_005332"
                     /pseudo
     CDS             complement(2204..2335)
                     /locus_tag="M2A35_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M85"
BASE COUNT          679 a          547 c          406 g          703 t
ORIGIN      
        1 aactaaaatt gtcatgcttt tgatattgaa tattttcctt tcgtaacatt ttaacattcc
       61 catcaatatc aaataacgtt gcaatctgct ccaaatctgt cttattttga gacatgcagg
      121 agaagtaatt acaaatatca tccagcaaaa tcggagtcaa tgctttatgc gtcatccagt
      181 cctcaaatct taactttctg gtgacgccct ctcctaaatc tgactgatac aagaatgcag
      241 gacgacaaga gttttctcct ttgggaatat cgacaatgta agcatgacca agcgcaccat
      301 cattaacacg taatacaaag ctcttacatt caggcataca ggacaggcgt tcctgaagct
      361 gctcgagcga tagtgtctgt tcattttcaa agcagtaact gaagccatcc ttgtccagaa
      421 atagtttcat aatagcatta gcggtaactc cgcaggaggg ttccacaatg cctttctctc
      481 gccctataat aacctcaacc tcctcaatcg acatccccca gtctgttgcc atctgtttga
      541 tgttagagtc gatattttgc attattgcag caggattttt ctgatgtata ggtacttcgt
      601 ggtcatcact atttagcaat acattagctt tgaggttctg tgagcacagg gaagcaaaat
      661 ctcttacatg taaggctgaa gtgtgttgca tgggagataa tacagcttca gtatttacag
      721 atatggcccc tgtcagacag gacgcggacg tcgggggggg aagggtaatg tctttcatat
      781 ctcattcatt cactctatgt ttatctataa atacaggcga ggtgcaaaag gaacttctgg
      841 catgggcatt atgtcatgtc tgaaattttt ctggtgcctg tatgccattc gccagtctct
      901 cagcggcatt gtccatttct gtgacgcggc ctggattgcc agccacacca ctttttacca
      961 actccatcca tgcaacaaat ccactacact ggataaaatt actaaatttt acttaatacc
     1021 acactaataa gatcttgctt tcctccatga taagattgca ttatttgatt ttcactccat
     1081 gaacgttcag aaatattata agatgatata tctattggat taaattcatt tctcgtgcga
     1141 tcatataaga catttgtctc tgttgtatcg ataaaaagaa ttcctttttg ctcaagcctg
     1201 tcaaacatat cataaatggc atgctcagcc tgtgccggca aggacgaaat atttaaaagc
     1261 gattctccat ttattttatc cattctaata ccaataatat cgccattatc gccatatatt
     1321 ttttctgcac tcccggcacc ataatattgg ttgaagcaac gaacttcgtt tgttacctct
     1381 tcattgcttt gagatgcagt aaacatcttc aggacttttg ttgcatcctc agcatcttca
     1441 tatactacag cgttaccccc tttgccaata acattaccag gcacaggcaa cctgttatag
     1501 tccacactcg gtaactctgg cggtgcataa tcaacaggag gtaaatcagg tctattcgaa
     1561 tgaacagcac ctctttccat agtcctattc actggcgatg aattcagcat cacctcaatt
     1621 ttcctgctaa tctctccctt aggccatccc agcctgtgca acagattagt aaagcaacca
     1681 ctatgacttt ctcttgtaac gcaaaactta ttatcagtga caacaacacg atatgttctg
     1741 ttgcccacct ttacttgcgc cccattatcc gagctagcag ctgcatccct tacagaggat
     1801 aaaatacgac tgtcaggaga agtcaggttt ctggttaaag aattccatga acaccccaaa
     1861 tttacagaag atggtgatag catacatttc aaccttcaaa atgaatcaat ctttactttc
     1921 ttaacaaaca tcaccatgac atgacaacaa aaaactggaa attaactatt gtttttgagt
     1981 atctggcaac tcattcatcc ttccagcatc ccgtcagacg aacgtttacg aactataaat
     2041 caggtattac tggccctcga aatctggaga tactgacgta tcaacacggt tcagtaacac
     2101 ccgatacctc ttccagactg tcaacaacga tgtctcttca tccgttgcga tctgcagcat
     2161 cctgaatatg aataccatag cttacggcat cacgaaaagt ttatgagcgg ctcaaagcat
     2221 caagtatagt attattaatc atatcgaccg tatgctgatc aatgagtctg ccacgactgt
     2281 catggacggc acgaattaca ctttcgtata cgttgcttaa atctgctgcg gaaag
//
LOCUS       DAHBHC010000246         2305 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHBHC010000246 DAHBHC010000000
VERSION     DAHBHC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.251"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..179
                     /locus_tag="M2A35_005333"
     CDS             <1..179
                     /locus_tag="M2A35_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCB7201466.1"
                     /translation="MPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKMPVTK
                     GGKLELTITGLGNE"
     gene            176..316
                     /locus_tag="M2A35_005334"
     CDS             176..316
                     /locus_tag="M2A35_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCB7201467.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            313..1002
                     /locus_tag="M2A35_005335"
     CDS             313..1002
                     /locus_tag="M2A35_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7201468.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSLVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELALLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTRTRSKQKA
                     TFSQQNIAKLD"
     gene            1324..1629
                     /locus_tag="M2A35_005336"
     CDS             1324..1629
                     /locus_tag="M2A35_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCB7201469.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            1616..2092
                     /locus_tag="M2A35_005337"
     CDS             1616..2092
                     /locus_tag="M2A35_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCB7201470.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            2089..>2305
                     /locus_tag="M2A35_005338"
     CDS             2089..>2305
                     /locus_tag="M2A35_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCB7201471.1"
                     /translation="MSRITAIISALVICVIVCLSWAVNHYRDNAITYKAQRDKNAREL
                     TLANAAITDIQMRQRDVAALDAKYTKEL"
BASE COUNT          592 a          519 c          609 g          585 t
ORIGIN      
        1 acatgccgga tcgccgtcgc cgtgacctgg ataatctgca aaaagccgct tttgacgcac
       61 tcaccaaagc aggtttctgg ctggatgatg ctcaggtcgt tgattatcgt gttgtgaaga
      121 tgcccgttac caaaggtggg aagctggagc tgaccatcac tggactgggg aatgaatgat
      181 gtttgagtct tatatggcag aacgtcttcg ccaccgctgg atgcgcctgc gcttatatcg
      241 ttttcccggt tctgttttga ccgattaccg gatactgaag aattacgcca aaacactgaa
      301 aggagctgcc gcatgaatac ccaatattta cagtatgtcc gcgagcaact cattgtggct
      361 actgctgatt tgagcggagc aacgaaaggc cagcttgaag cctggctgga gcatgcacaa
      421 tttgatactg gtacatacaa acgaaagaag ccgcgcattc tggatgaggt aactggcagg
      481 atgattacgc tggataatcc gccgatttcc ggtaagcagt cgtacgcaaa aggttcatcc
      541 attgcactgg tcagcctggt tgagttctca acctcgtcat ggcgccgcgc ggttctgtct
      601 ctcgaagaac atcagaaagc gtggttgctg tggagttaca gcgaaagtgt tcgctgggaa
      661 catcaggtca ccataactca gtgggcatgg agcgagttta agactctgtt gggtaccagg
      721 aaaattgcag gtaaaacact ggaacgtttg aagaagttga tctggctggc ggcacaggat
      781 gtgaagaacg agctggcagg gcgtaagacc tatgaatacc aggagctggc attactggtg
      841 ggagtgacat caaaaaactg gtctgagaca tttactgaac gctgggttgc aatgaagcac
      901 atttttctac agcttgatag ccaagcttta ttgcttctaa cgagaacacg ttcaaaacaa
      961 aaggcgacat tttcacagca aaatattgca aaactggatt aaaaagcata tattttatat
     1021 aaatctgata ttttgccaat gttgtacgca ctggcagtaa tccaaattca agctcgaggt
     1081 ttaaagcctt gggcttttct gtttctgggc ggtgagtatc cttccaacgt accccagcca
     1141 gggtgtcttc agctgttgag ttgatattgc ttaaccctct gttgccagct acatgctggc
     1201 ttttttattc caggcttgcg gggagcatca actccgtgct ttgtcgttaa attaccccgt
     1261 gagcctgatt tctgacattt aacgtcccgg ccttttgtcg gcggcgaaac attggctatt
     1321 catatgcacg aaaaagagag ccttgccgga gcgttctggc tcgttttgct gatcatcgca
     1381 ggttggggcg gtctggtccg ctacctgata gatgtgaagc agagtaaagc aacgtggagt
     1441 tggataaatg ctctggctca aatagtggta tcaggattca ccggtgttat tggtggcctg
     1501 atcagcatcg aaagtggatt cagtatttac atgattctcg cgacagcggg gattagtggt
     1561 gcgatgggtt cggttgcact gacgtacttc tgggaacgac tgacaggggt gaaaaatgca
     1621 aaatcttaat cctcagcgta aagccttcct cgatatgttg gcgtggtcag aaggaacgga
     1681 taacgggcga caaccgacac gtaaccacgg ttatgatgtt attgttggcg gcgaactgtt
     1741 tactgattac tccgatcacc ctcgcaaact tgtcacgcta caccccaaac tcaaatcaac
     1801 agccgccgga cgttatcagc ttctttcacg ctggtgggat gcttaccgta aacagcttgg
     1861 tttgaaagac ttctccccca aaagccagga cgcagtggca ttacagcaga ttaaagagcg
     1921 tggtgcttta ccgatgattg atcgcggtga tatccgtcag gcaatcgacc gttgcagcaa
     1981 tatctgggct tcactgccgg gcgctggtta tggtcagttc gagcataagg ctgacagcct
     2041 gattgcaaaa ttcaaagaag ctggcggaac gctcagagag agtgaggtat gagcagaata
     2101 accgcgatta tctccgctct ggttatctgc gtcatcgtct gcctttcatg ggcggttaat
     2161 cattaccgcg ataacgcgat tacctacaaa gcccagcgcg acaaaaatgc cagagaactg
     2221 acgctggcga acgcggcaat tactgacata cagatgcgtc agcgtgatgt tgctgcgctc
     2281 gatgcaaaat acacgaagga gttag
//
LOCUS       DAHBHC010000247         2297 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHBHC010000247 DAHBHC010000000
VERSION     DAHBHC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.252"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            270..797
                     /locus_tag="M2A35_005339"
     CDS             270..797
                     /locus_tag="M2A35_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201472.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDEKNWYEEVKNF
                     QPDTIKIVYDKNQIIVAITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVV
                     KRIYTTDEQQQQAESQKAALLSEAESIIQPLERAVRLNMATDEERARLESWERYSVLV
                     SRVDTANPEWPQKPD"
     gene            complement(826..1359)
                     /locus_tag="M2A35_005340"
     CDS             complement(826..1359)
                     /locus_tag="M2A35_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201473.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYGHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVV
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLFAQKV
                     ESTDTSNLPVTFPEQPE"
     gene            complement(1362..>2297)
                     /locus_tag="M2A35_005341"
     CDS             complement(1362..>2297)
                     /locus_tag="M2A35_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201474.1"
                     /translation="QASGALQKNQNGADIPGKDTFTKNIGACRAYSAWLDIGGDSQVW
                     TTAQFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAM
                     TIRVTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGND
                     KAVGWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGF
                     EADWSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYV
                     VTGSVNGNRDELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          609 a          537 c          507 g          644 t
ORIGIN      
        1 gaactggctt tgcggctcgc aagttattcc cggcaactat ggcaattttg attccagata
       61 tgtgaaagat gttcgacttg gttcacagca atattatgga gtgaacaact ggcaaacatg
      121 gaatttccag tgcccgtcag gtcatgtatt gtctggtatt aatgttcagg atacagggtc
      181 caactctgcc gataatatag cgggcgttta ttacagaccc gttcaaaagt atataaatgg
      241 cacctggtat aatatagcga gcgtttaata tgatgcactt aaagaacata aaagcgggta
      301 atgctaaaac actggaacag tatgagttaa caaagaagca cggagtcatc tggctttact
      361 ctgaggacga aaaaaactgg tatgaggaag tgaaaaactt tcagccagac accataaaga
      421 ttgtttacga taaaaatcag attatagtcg ctatcaccag agatgcttca acgcttaatc
      481 ctgaaggttt tagcgttgtt gaggttcctg atattacctc caaccgacgt gctgacgact
      541 caggtaaatg gatgtttaag gatggtgctg tggttaaacg gatttatacg acagatgagc
      601 agcaacaaca ggcagaatca caaaaggccg cgttactttc cgaagcagaa agcattattc
      661 agccactgga acgcgccgtc aggctgaata tggcgacgga tgaggaacgc gcacgactgg
      721 agtcatggga acgctacagt gttctggtca gccgtgtgga tacggcaaat cctgaatggc
      781 cacaaaagcc tgattaaaaa ttaaggcccg ctatcgggcc ttgtctcatt caggttgttc
      841 gggaaacgtt actggcaggt tggaagtgtc tgtagattcg actttctgcg caaagagcat
      901 ccactctgtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta actgtgagtc
      961 ccatagctgg gttttatccc tgacgagctg tagcaggctt tgcttttcat tttccgcctg
     1021 ctgcctctgc tcttcctcgg tataagttcg ctttaccact acgccatctt tgaacatcca
     1081 tttacccgaa atgtcagcac ggcgatttgc tgtaatatca ggtaattcaa cgacgcttgc
     1141 gccttccgga ttaattgctg aaacatcctt ttcaatacaa ataataacgc cgttatggcc
     1201 atagaccatt ttcaaagtgt ctggctggaa attcttttgt tcctcatacc agttttttcc
     1261 atcatctgaa taaagccatt tgatgttaaa ttgctttgtt agctggtatt gctcttttgt
     1321 tttagggttg ccagcagtaa tgttttttaa gtgcatcatc gttaaatact ccccgcgtta
     1381 taccacgtcc cattaatgca atactgaatt ggccttgcct gagttgtatc aattaattca
     1441 tcacggtttc cgttaactga acccgtaacg acataacctg acctgtcaga ccagccgggg
     1501 ccattccatg tctgaacaga tgacagaccg ccaaggcgaa tacctgtaat aaaccttgag
     1561 ttacattctg cctgcgtgta tgcaccaaca tctcccgcag agggttttcg ggtcgtggtg
     1621 taaaactctg accagtcagc ctcaaatcca taaccatcac gcgctgaacg ataaaaaata
     1681 ccgccgttcc tgtaattcac gcggaactgt acagcagggc agctccccgt attcatattg
     1741 aagtggagga ttaatgtcga tgcgccacca atatttgcgt tatagacccc gctattccag
     1801 ttccagccaa cagctttatc atttccgaca gtgcttcctg tttgccctaa agcaaatgca
     1861 ggttgttgat ttttcgtgtt gtagtctcgt cgccagccag gagcgtaagc atcaccatga
     1921 ttaatataag tgaattgagc gttagtaatt cctccaccgc tggacgtgct cggcgtggta
     1981 acgcgtatgg tcattgcgcc gcgagtgcca ataacttcca ccacagcacc tgcaagacaa
     2041 atatttccgc aacctgtatc tgtaatgacc ttattatttg cataagccca tgagcctttg
     2101 cacatccagt aaggatggtt aaatgctccc tgactctcca gccacgaaat aaattgcgcg
     2161 gttgtccaga cctgactatc gccaccaata tccagccatg cgctatatgc gcggcaggcc
     2221 ccaatatttt tggtgaaggt atcttttccc ggaatatctg cgccgttctg gtttttctgt
     2281 aatgcgccag aagcctg
//
LOCUS       DAHBHC010000248         2282 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHBHC010000248 DAHBHC010000000
VERSION     DAHBHC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.253"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..161)
                     /locus_tag="M2A35_005342"
     CDS             complement(<1..161)
                     /locus_tag="M2A35_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HCB7201475.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVV"
     gene            complement(163..381)
                     /gene="ccdA"
                     /locus_tag="M2A35_005343"
     CDS             complement(163..381)
                     /gene="ccdA"
                     /locus_tag="M2A35_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCB7201476.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            980..1210
                     /locus_tag="M2A35_005344"
     CDS             980..1210
                     /locus_tag="M2A35_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCB7201477.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVRELEIIREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            1207..1623
                     /locus_tag="M2A35_005345"
     CDS             1207..1623
                     /locus_tag="M2A35_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HCB7201478.1"
                     /translation="MNKTYMLDTCICSFIMREQPETVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(1682..2122)
                     /locus_tag="M2A35_005346"
                     /pseudo
     CDS             complement(1682..2122)
                     /locus_tag="M2A35_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          598 a          574 c          562 g          548 t
ORIGIN      
        1 accaccgggt aaagctcacg ggagactttg tctgacagca ggcgggcgct ggccaggggg
       61 atcaccatcc gtcgccctgg cgtgtcaata atgtcactct gtacatccac aaacagacga
      121 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagt ccctgttctc
      181 atcggcaaaa gagccgttca tttcaataaa cctggcgacc tctgccatcc cttcctgatt
      241 ttcagcctgc cagcgttcag cgcgcagtcg ccgggcttcg ttctgcatgg tcgtactgac
      301 cagaccggag atattcacgt catacgcctt gagcagctga tagctgtcgc tgtcaactgt
      361 cactgtaata cgctgcttca tagcacacct ctttttgaca tacttctggt atgcatatca
      421 gtatatattc ttatgccgca aaaatcagcg cgttaataca catactgtta tctggctttt
      481 agtaagcctt atttatttta ccttgcatta tgttaaccaa taaaaattaa aatcagactt
      541 ataaaaacaa agcgtaatta tcgaaggacc atttccggca gatccgattt ttctgttatt
      601 gaggggtttc cggaagtgta ccacgcagag gaaatcatca tatcgctgtt ttccgcccct
      661 gcacggcgca tcacaggtca gggaaataac acaacatata gtgtcatgag tagcaaaatg
      721 aagcactaca tgttgtactt acaggaggtg gcgttgtgtg ggggagagga agatccgtga
      781 caaccggaaa ccggatctcc gataaaaaag aaagaaatag agatatgtta agcatatgta
      841 tatgcatatg cctggcatct acatatgcta cagctataca tatgtcacac atctgcctat
      901 actaaacata tgcacacacc cggtgctttg aatattccgg atatgtttca catatccata
      961 tgctcgggag agaaagaaca tgagaaccgt atcgatattt aaaaatggca acaaccgcgc
     1021 catccgtctg ccccgtgatc tggattttga gggggtgaga gagctcgaga tcatccggga
     1081 aggggacagc atcatcctgc gccccgtccg gccaacctgg ggctcattcc tggagtacga
     1141 aaaagccgat ccagatttta tggcggagcg cgaggatgtt gtcagcgacg aagggcgatt
     1201 taacctgtga acaagactta tatgcttgat acctgtatct gctcgttcat catgcgtgaa
     1261 cagccagaaa ccgtcctgaa acgtctggag caggcggtgc tacgcggcca ccgtatagtg
     1321 gtctcggcca tcacctactc tgaaatgcgc ttcggggcta ccggcccaaa ggcctcacca
     1381 cgtcacgtcc aactggtcga tgaattctgc gcccgcctcg atgccatcct gccctgggat
     1441 cgcgccgcag tagacgccac cacgaagatt aaagtggcac tgcggctggc cgggacgccg
     1501 atcggcccga acgacacggc gattgccggg cacgccatcg ccgccggggc gatactggtg
     1561 acgaataata caagagaatt tgagcgggtg cctgacctgg tgctggaaga ctgggtgaag
     1621 taagacactc ttttcgaagt caggagataa gggcaccagg aaacagaccg tatacgggct
     1681 gtataaccct gcgaagaaga gtatcagcac tgcaacggag acccactgcc gttgcagccc
     1741 gtttcccggc ctcacctccg gcgattaatc ccagttgacg ttgtaaattt tgtaacgcct
     1801 gtgaagactg ttagtgtgaa ccggcgaagg gagcaagtga ctcggcaaaa attttacgtg
     1861 aacaagccgg gttacggcag taccagtagc ggacggaaaa tacaagccat agcgtatgtc
     1921 ctgagcaggg tagatgctgt atccggcgcc gacgacaacg atgaacagag tcgctacgct
     1981 taaagcattc aggacattgt gctcttttac gttttccgtg gagatggagg gttataccgt
     2041 cagagctaat aatttgtcga ctgcatcgcc atccgcaaga taactgtagc agtgtcttaa
     2101 gagagtgcat tgagttgccc ataacttaaa acagcttaga tctttgttca ccagaaagtt
     2161 tcatccattt tcttccaccc aggttgcgga agaaccactt tatccgtcgt tgagagtatt
     2221 ccggagcagc tggacttcat tttcggacag atagcgtcgc gacacgttat catctcctgt
     2281 ca
//
LOCUS       DAHBHC010000249         2248 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHBHC010000249 DAHBHC010000000
VERSION     DAHBHC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.254"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1853)
                     /gene="ag43"
                     /locus_tag="M2A35_005347"
     CDS             complement(<1..1853)
                     /gene="ag43"
                     /locus_tag="M2A35_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCB7201479.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGANRLGHFSV
                     GNGMADNVVLENGGRLDVLEGHSAQNTLVDDGGTLAVSAGGKATDVTMTSGGALIADS
                     GATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTQDAVLSRAVAKGDSPVTFH
                     KLTTNNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRA"
BASE COUNT          469 a          663 c          560 g          556 t
ORIGIN      
        1 gcacgataag cattttcact gcgcaggtac cagtcttcat cgctgtcacg gttcagggtg
       61 tagttaaagg cgccggcctg aagcgggcga ctcagggcaa acgcaccttc ttctgtggtg
      121 gcaccattct gtgcatccac aacccggata ccctgtccgg aggttgccac cccgaggtta
      181 ctgtttccga catttgtaaa cgcaagccag gttttgccgg ttgcctgacc accattaatc
      241 accaactggt cagaggcatt gctgccatca aggcgaacac gcatattgat ggtgccaccc
      301 tgaccggtga ggttattggt ggtcagttta tggaacgtta ccggggagtc gccttttgca
      361 acagcacggc tcaggacggc atcctgtgtc gtctgattat caaagcgaat ctcccctgcg
      421 ttaacaatat cgccggtact gaccacatca ccattcagta ccatactggc gcctttactg
      481 agctgtgttc tgccactcag acttcccccg gcagccagag tcagtgttcc acgatgtccg
      541 acagtggtgt tgccagcctg tcccccggca ttaaccgtaa agctgccgcc attttccagc
      601 agcaggccgc tggcctgacc ggatatgcca tcaatactga acttaccgct ggcattggtc
      661 ccctcaacag tggcaccact gtctgctatc agggcaccac cggatgtcat ggtgacatct
      721 gttgccttac caccggcaga cactgccagg gtaccgccgt catccaccag ggtattctgc
      781 gctgaatggc cttccagtac atccaggcga ccgccatttt ccagaacgac gttatctgcc
      841 atgccatttc ccacggagaa atgccccagg cggtttgcgc cggtgacagt tgcagcagta
      901 ctggtaacca gtgcaccgcc cgtgttctgg gtgactgcag tggcttcccc gccggcatgt
      961 accctcagtt caccgttctg atttatggtg gtgttaccga cagcaccacc tgccttaaca
     1021 acctgccagc ccccctcgtt aattaccgtg ttcagcgcaa gtccgtcctt gtgcacatac
     1081 tggtaaccgc cattcagtgt ggtattcagg gcccttccgt gtaccgactg gtcaccacct
     1141 gcgtaaatga gagtgtcacg ggctatcccg gaaaataaga taatctgccg tccatttttg
     1201 ttgatggtgg tggattctgc tgttccctgg accttctgac ccgtgtacga gttgtcagaa
     1261 tccgggccgc cttctgcgcc ggtgttgatg atggttcctg ttgccagccc gccactttta
     1321 acgctctggt aaccgtcgcg gttgataatt gtaccggtgg caaccccgcc ctcatgaacc
     1381 cactgctcgc ctctgttatt cagcgtggtg ttcaccgcca gcccgttcag gctctgtccc
     1441 ccgccgtcac gaataaccgt atcactggct gttcccccct ccagcacgac ctgacgacca
     1501 tttgtggtga cagtggtgtt gccggctatc ccgccattct gtatccattg cccaccggtg
     1561 ttttcttcac tgtccggccc cagttccagc ccggtactga tggtcattcc gttggccgta
     1621 ccgaggacaa tctggttgtc atgatttgtc agtgttccgc cgttcacggt ttccccagcc
     1681 tgtacaaccg tgtcagcagc cagtgccggg actgacgtga cagcagcaag agacagagca
     1741 accgccacac cggcgcgttt tccccgtgag cgggccagtt cggaagccac caccagggtg
     1801 cccgtaatgt gattccatac cagcctgtag ctggtgttca gatgtcgttt catcagcttt
     1861 tccttacaga gggtgaataa aaaagccggt acccacaaaa gtggataccg gcaggtacaa
     1921 aacagagtta tcgggcacag cgatacgtcc cgggcgtaaa cgctctcacc cttcatcagg
     1981 cagagaagta tcagtccgtt gtgtgcaatt ccgttttgca tgatgaagag attcagctgc
     2041 cttttcgcag gccattaacc actataaacg atcgatgcca gcgataaata cctgttttat
     2101 tgatcgtttt attctattaa taacaaggca gccacgcaag agaaacagga ggagtatgag
     2161 gagtttgtgt catgttttca gttggttaac gtaatgtaat caggcatttc ccctggcaga
     2221 gtgttcagaa gaaaatccag ttccatac
//
LOCUS       DAHBHC010000250         2221 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHBHC010000250 DAHBHC010000000
VERSION     DAHBHC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.255"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(499..948)
                     /locus_tag="M2A35_005348"
     CDS             complement(499..948)
                     /locus_tag="M2A35_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201480.1"
                     /translation="MQEEANKILVDLLKKASDGIDSAIAFSQAQIPDVVHQLLVWNMV
                     DSLIKTLIAISTIPLVIWFMKKQYQKVEIGKFDNEGWSWEKGKPKYKPTMIWESNGEI
                     SFLILPLAAVFVLWVSFIIAVVTNMTWLKIWLAPKLYLIEYAASLIK"
     gene            complement(1073..1933)
                     /locus_tag="M2A35_005349"
     CDS             complement(1073..1933)
                     /locus_tag="M2A35_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201481.1"
                     /translation="MKELQKINSIMERLSALERKLDDCGKLCVEKQNELYREFFTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFSVKIQERVSGSVTRDMAEQLVAMSNKTKRKKA
                     LLVTANQLSGRVDFQVRQGGRVLVNDAFVGRCFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDVQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSAPEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRVIPVPDVVIIDDVEKCKSVSSTATIKRILRCSSS
                     AVVFRKQLHN"
BASE COUNT          663 a          519 c          420 g          619 t
ORIGIN      
        1 tcattccacg gacaaaaaca gagaaaggaa acgacaaagg ccaaaaagcc cgttttcagc
       61 atctgtcgtt tcctttcttt tcagggggtg ttttaaataa aaacatgaag ttacagcgaa
      121 gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc cgcgcgcctt
      181 ccgccccgta acgttctgga ttgccggaaa ggacccgcca gccattcggg tttacttcgc
      241 aatgggattt aatatctgaa cgaccaaagt cgtgcgacca cggtcgcaca gcaatgaatc
      301 aacatcctgc atgctcgtgc agaggagttc cccgtcaggc tacggtcatg gttaatgcgg
      361 gaatacagcg acgatacagc gcatgatgta tcaggcttga atacatttat ccgttaaaag
      421 ggatatcagt taagttatcc cgtgtagggt ataagccatt atcaaagcca ctcagtagcg
      481 aatggctttt gtaatggact atttaattag tgatgctgcg tattcgatta gatataactt
      541 aggtgccaac cagattttta accatgtcat attggttact acagcaataa taaaacttac
      601 ccacagaaca aaaactgctg ctaatggtaa gataagaaaa ctaatctcac cgttactttc
      661 ccaaatcata gtcggtttgt atttaggctt ccctttttcc catgaccatc cttcattatc
      721 gaacttacca atttcaactt tttggtactg tttcttcata aaccaaataa ccagcgggat
      781 tgttgaaatg gctattaatg ttttaatcag gctatcaacc atattccata ccagcaactg
      841 atgaacaaca tccggaatct gagcctgact gaatgcaatg gcggaatcaa ttccatcgct
      901 ggccttcttc aataaatcaa caagaatttt atttgcttct tcttgcatgt ttatatcctg
      961 taagttgtaa gcctgttcga gaaaaataaa caactacgag agacagaaaa aaatatctca
     1021 ggctctcttt ctataagctt ttagttgagg atgttctgca aaacaaaact ggttagttat
     1081 gtaactgctt ccggaataca acagccgacg agctacaacg taaaattcgt ttgattgtgg
     1141 cagttgagga aacagacttg catttctcaa catcatcaat aatgaccaca tccggtacag
     1201 gaatgacgcg ggtatgcaat gtttttgcac taataacatc acagccattc agtggtcgaa
     1261 tatctgcatc ttcaggcact accagaagca cacgtttttc cggtgcagaa aagccagcag
     1321 caagagtgcg tgacaataaa gtcattccgc tgcgtggctt catacaaatt tcagaaatat
     1381 gattcttttc gtccaaatga tgctggacat catttttatc atcactcaac aactctgctg
     1441 ttaatacaat ccctgctgtc tcagatttcc aggaaatgct gatatcctga tcatctgcat
     1501 caacatcata tttcatcgag taaggactaa agcaacgtcc aacgaacgca tcatttacca
     1561 gtacacgccc accctgtctg acctgaaagt caacgcgccc tgaaagctga tttgctgtta
     1621 caagcaatgc cttttttctt tttgttttat tgctcattgc caccaactgt tcagccatat
     1681 ctcttgtcac actaccagaa actctttctt gaattttcac agagaatttt ctggatgtag
     1741 tagcatcgac attaaaaaat ctggatgtca tcaggcagtt attaacaatt ggcatcagct
     1801 cacttacgat tgtaaaaaat tcccggtaaa gctcattttg cttttcaaca cacagctttc
     1861 cacaatcgtc cagcttacgc tcaagagcac ttaacctttc cattattgaa ttaatttttt
     1921 gcaactcttt catcaaaacc tcctgacaaa gataccgtgc gaccacggtc gcacgctcct
     1981 gaatacatgc cctgtttctt ccaccctcgc acaagactgg cgagcatgag ggacaaaccc
     2041 gcgaatcatt agcgcggtaa aaacccggtg tgcatcgttt tgattattcc cgcacactcg
     2101 cgcagaagga gttccccgtc gggctacggt catggttaat gcgggaatac ggcgacgata
     2161 cagcgcagct aaaagggtaa tggacggata gaccggttta tttcattcca caggattctg
     2221 a
//
LOCUS       DAHBHC010000251         2191 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHBHC010000251 DAHBHC010000000
VERSION     DAHBHC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.256"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..270
                     /locus_tag="M2A35_005350"
     CDS             <1..270
                     /locus_tag="M2A35_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCB7201482.1"
                     /translation="RQVEWHGRWLDAESWKCVFTAALKQRDVVPNLAGSGFVVIGQST
                     SRMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            267..794
                     /locus_tag="M2A35_005351"
     CDS             267..794
                     /locus_tag="M2A35_005351"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCB7201483.1"
                     /translation="MTIKSNTPASDRYRVIEDYLYLDGDTVRYKKDSLKHPNHSHRAG
                     DEIKTSINGSGYRQVCFAGIQMFVHVVVFALHNKRMPLKNIDHINGNRLDNSPKNLRE
                     ASRIANSRNQKTKCNSRSGIKNVLWNKQKNKWAVQVRTDFGRLHFGFYEDLELAGLVA
                     SEAINKYHGQYARVE"
     gene            791..1321
                     /locus_tag="M2A35_005352"
     CDS             791..1321
                     /locus_tag="M2A35_005352"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCB7201484.1"
                     /translation="MIKHKSETPKEVRDCWQTPLWLFDALDIEFGFWLDSAASDKNAL
                     CAHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMS
                     VGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTV
                     SKAALMAIGQGVRRAA"
     gene            1318..2163
                     /locus_tag="M2A35_005353"
     CDS             1318..2163
                     /locus_tag="M2A35_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HCB7201485.1"
                     /translation="MINVVSFSGGRTSAYLLWLMEQKRRAGEDVHYVFMDTGAEHPKT
                     YEFIRNIVSNWKIDLHCLRVIPNPEMGKASSYEEIGVTDIGPDLIPWKRMLNKYGHPY
                     IGGAFCTDRMKSVPFTKYCQEKFGKGNYITWLGIRTDEPNRLKRANGFRYLADISDFE
                     KQDVLDWWSEQKFDLGIQEHLGNCVFCIKKSMQKVALAAMDEPELADAFINILDTEIK
                     TGKEPVMYRGNNTLKSLIALFSDISRDELASRMTSMRQYDSGSCSESCEAFSCQLGFN
                     FEDAA"
BASE COUNT          658 a          399 c          593 g          541 t
ORIGIN      
        1 cgtcaggttg aatggcatgg tcgatggctg gatgcagaaa gctggaagtg tgtgtttacc
       61 gcagcattaa agcagcggga cgttgttcct aaccttgccg ggagtggctt tgtggtaata
      121 ggccagtcaa ccagcaggat gcgtgtaggc gaatttgcgg agctattaga gcttatacag
      181 gcattcggta cagagcgtgg cgttaagtgg tcagacgaag cgagactggc tctggagtgg
      241 aaagcgagat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca gcgtcagata
      301 ggtatcgggt tattgaggat tatctgtatt tagacggaga tacagtcaga tacaaaaagg
      361 actcgctaaa acaccccaac cacagccacc gggccggaga tgaaattaaa acatcgataa
      421 atggatctgg gtatagacag gtgtgttttg caggcattca gatgtttgtt cacgtagttg
      481 tttttgcgct gcacaacaaa agaatgccat tgaaaaatat tgaccatatt aacggaaaca
      541 ggctggacaa ttccccaaaa aatcttaggg aggcaagccg gatagcaaac agccgaaatc
      601 agaaaactaa gtgcaatagc cgttctggaa taaaaaatgt tttatggaac aagcaaaaaa
      661 ataaatgggc cgttcaagtg cgcacagatt ttggccggtt gcattttggg ttctatgaag
      721 atcttgagct tgctggtttg gttgccagtg aggctatcaa caaatatcac ggacaatatg
      781 cgagggttga atgattaagc ataaatcaga aacaccaaaa gaagttagag actgctggca
      841 aacgccgctt tggctttttg atgcactgga tattgagttt ggattctggc tggattcggc
      901 agcgagcgac aaaaatgctc tgtgcgctca ctggctaact gaggctgacg acgcgctaaa
      961 ttctgagtgg gtaagccacg gtgcaatctg gaataaccca ccgtacagca atatcaggcc
     1021 gtgggtggaa aaagccgctg agcagtgtat acaacaacga cagacggtag tgatgcttgt
     1081 gccagaggat atgtcagtcg gatggttcag caaggctctg gagagtgtcg acgaagttcg
     1141 tattatcact gatggacgga ttaattttat cgaaccatcg acagggctgg agaagaaggg
     1201 aaacagcaaa ggttccatgc tgctgatttg gcgaccgttc atcagtcctc gacggatgtt
     1261 tactaccgta tccaaagcgg cattgatggc gatcgggcag ggcgtcagga gggcagcatg
     1321 attaatgtcg ttagtttctc aggtggcagg acgtcagcat atttgctctg gctaatggag
     1381 caaaagcgac gggcaggtga agacgtgcat tacgttttca tggatacagg agcagagcac
     1441 cctaaaacat atgagttcat tcgaaatatc gtcagtaact ggaaaataga cttacattgc
     1501 cttcgtgtaa tacctaatcc agaaatgggg aaggccagta gttatgaaga gataggtgtc
     1561 actgatattg gtccagatct tataccgtgg aaaaggatgt tgaataaata cggacatcca
     1621 tatatcggcg gtgcgttctg taccgacaga atgaagtctg ttccattcac caaatattgt
     1681 caggaaaagt ttgggaaagg aaattacatc acatggcttg gcattcgcac agatgaacca
     1741 aacaggctta aaagggctaa tggttttcgg tacctggctg atataagcga ttttgaaaaa
     1801 caggatgtgc ttgattggtg gagtgagcag aagtttgatc ttggcataca ggagcatctt
     1861 ggtaattgtg ttttctgcat aaaaaaaagt atgcaaaagg tggcgctggc agccatggat
     1921 gagccagaac ttgcagatgc atttatcaac atccttgata cagaaataaa aacaggaaag
     1981 gaaccggtta tgtatcgagg gaataataca cttaaatcac tgattgcctt gtttagtgat
     2041 atatcaagag atgagttagc ctcaagaatg acatcaatgc gacagtatga ttcaggttcg
     2101 tgttctgaat catgtgaagc attttcatgt cagcttggat ttaattttga ggatgcggca
     2161 tgaggcgaca gcgacgaagt atcaccgaca t
//
LOCUS       DAHBHC010000252         2169 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHBHC010000252 DAHBHC010000000
VERSION     DAHBHC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.257"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..674)
                     /locus_tag="M2A35_005354"
     CDS             complement(<1..674)
                     /locus_tag="M2A35_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HCB7201486.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAG"
     gene            complement(676..1464)
                     /gene="paaB"
                     /locus_tag="M2A35_005355"
     CDS             complement(676..1464)
                     /gene="paaB"
                     /locus_tag="M2A35_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HCB7201487.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLECDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(1464..>2169)
                     /locus_tag="M2A35_005356"
     CDS             complement(1464..>2169)
                     /locus_tag="M2A35_005356"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HCB7201488.1"
                     /translation="ARNALNNALLTQLVNELEAAATDSSISVCVITGNARFFAAGADL
                     NEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGYALGAGCELALLCDVVVAGENA
                     RFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSGESITAQQAQQAGLVSDVFPSD
                     LTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLAQERQLFTLLAATEDRHE
                     GISAFLQKRTPDFKGR"
BASE COUNT          477 a          636 c          547 g          509 t
ORIGIN      
        1 ccagcgccat cgcgaagtgc agcgtcaatc acttctggtg cagcaacctg ctcttccagt
       61 gcccgccagg cctcggaata ataaggacgc gccacacggt taacgataaa tccaggggtc
      121 gaatgacagc gcacaggctg cttaccccaa ctcaacgtta gttcacacaa ctgctcaaca
      181 acttccgctg ccgttgccag cccactgacc acctccacca acttcatcac cggtgccggg
      241 ttaaaaaaat gcagccccgc aacacgttca ggatttttta cctccgcagc aatcgcggtt
      301 atagagattg acgaagtgtt agtggtcaat agcgtttgtg gcgggcaaac ttccgccagc
      361 tgtgcaaaga gcgctttttt gacttccaga cgttcagacg ccgcttcaat gaccaggtcc
      421 gcagctgcca gcgcgtgaat atcggtcacc gggatcaggc gtttcaatgt gcgttcacag
      481 gtttcagcag tcagttttcc ccgcgtcacg cgtgaattta gccgcgcgtg tatcccgtcg
      541 attgcgcggg tcagcgcttc agcagaaatg tcatacagta aaacctgatg tccatgactg
      601 gcggcaactt cagcaatgcc agcccccatg gtgccgctcc caatcactgc cacagtttgc
      661 acatttatca tcatgctatt tccccgtgaa ctgcggtgag cgtttagcca ggaacgcact
      721 gacaccttca cgataatctg cgctccgtcc ggcaagtcgc tgatagtcgc attccagatc
      781 cagttgcgta tcgagagtat tggtttcagc gctatttatc gcttgcttga taagtccaag
      841 accaaatgtc ggttgtgtcg ccagatgccg tgccagctgt tgcgcggtat ctgccagcgt
      901 ttcatcatca acaacctgcc agatcatccc ccattcgtgc gcctgttcag cactcagttg
      961 attccccagc agtgccagcc ccatggcgcg cgctcgccca gcaacgcgtg gcagtaacca
     1021 ggttccaccg caatcgggta ctaaacctaa cttactaaac gccatgacga attttgctga
     1081 acgggcagca ataacgatgt cgcatcccag tgccagtgtt gcgccagccc ctgccgccac
     1141 gccattgact gcacagatca ccggttttgg cagttttgcc aggcgacgta ccagtgggtt
     1201 atagaaacgt tcaactgaca tccctaaatc cggtgcgggg cctgtgggat caacgttacg
     1261 atcgttaaga tcctgaccag cacaaaatcc gcgcccggca cctgtaagta acaggcaacg
     1321 gatagtgtcg tcgcgctcga cctgtttcag gcactctgcc agttgtgcgt gcatctcatc
     1381 attaaaactg ttcaggcgtt ccgggcggtt gagcgttagt gtcatcacgc ccttttctac
     1441 atgactgagg atgaattcca tcattagcgt cctttaaagt cgggcgtgcg tttttgtaag
     1501 aaagcggaga tgccttcatg acgatcttct gttgccgcca gcaaggtgaa taactgtcgc
     1561 tcctgggcaa gtccggcttg caaagccact tcctgcgact ggcgcagcgc ttgctttgcc
     1621 gcttgtaagg ccagcggcga gtgacgtgcc attttcgacg ccagctgtaa ggcgtattcg
     1681 agggttaaat cgctggggaa gacgtcgcta accagcccgg cctgctgtgc ttgctgagcg
     1741 gtgatacttt ctccgctcag caccattttg ctggctaacg atttaccgac actacggatt
     1801 aaacgttgcg ttcctcctgc gcctggcatg atgccgagag tgatttccgg caaaccaaaa
     1861 cgcgcgttct ctccggcaac caccacatcg cacaacaatg ccagttcgca acccgcacca
     1921 agcgcgtaac cgttgacggc tgcgatgaga ggtttgttga aggcctgcaa tcgcgcccat
     1981 agctgcgggc gtgtatcgtt taaggtggcc gcgagatctt tttctgccat ttcgttgaga
     2041 tcggccccag cggcaaaaaa gcgtgcatta ccggtaatca cacagaccga aatgctgcta
     2101 tcggtagccg cagcttccag ttcatttacc agttgcgtca gaagggcatt atttagcgca
     2161 ttacgtgcg
//
LOCUS       DAHBHC010000253         2131 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHBHC010000253 DAHBHC010000000
VERSION     DAHBHC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.258"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(231..1006)
                     /locus_tag="M2A35_005357"
                     /pseudo
     CDS             complement(231..1006)
                     /locus_tag="M2A35_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1227..1667
                     /locus_tag="M2A35_005358"
     CDS             1227..1667
                     /locus_tag="M2A35_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCB7201489.1"
                     /translation="MSKKKFSVENEDLSTVRTSLLNSNLVRKLIMEMSDEDFNQVKDN
                     LEKAIEQRNELKDSLAIIKKYMDDAGVTAEDVYREFGKKLQTGRKNDSGESNKYRYEY
                     NGQIMYWSGQGRRPSVIAEAIKAGGSLDDFLISQGTSSSCSDYE"
     gene            1936..2061
                     /locus_tag="M2A35_005359"
                     /pseudo
     CDS             1936..2061
                     /locus_tag="M2A35_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          568 a          457 c          477 g          629 t
ORIGIN      
        1 tgtcgttgaa ggacaggtgt atggcaatga gccgctttat tatcgagttc ggtgactgcc
       61 tgtcacttct gaaaaaaggc atttacacag aatcctaaac aggttccgac attccccact
      121 catactaaac accttgtcac ttgacgcagg gctgaacatt ttacaacaac ggtataccaa
      181 tatgatattg gtttactaaa ttttttggga atccctcagg gaatttctcc ctatacttcc
      241 agacatctgt tatcatttaa cccattactg atccgctgcc gcatatatac ctgcggcgag
      301 cgataactta gaactatgct gcattgtaat gttcgattgc tactgcatgg ttatgcaatg
      361 ccgtcattaa catctggctt gggcatgaac acgatgtagt cacgctttat cgttttcacg
      421 aagctctctg ctattctgtt actctccgga ctccgcaccg ccgtgttctt cgattcaagt
      481 cccaacatcc gagcgaactg gcgtgtttca ttagcccggt agcatgaacc attatctgtc
      541 agccactcca ctggagacgc cggaagctcg ttgccgaagc ggcgttccaa cgctcctggc
      601 atgacgtcct gtactgtttc actgttgaag ccgccggtag tgaccgccca gtgcatcacc
      661 ccggcaataa gggagcgtgc gctatccact ttttgcacgt ccatattcaa cggcttcttt
      721 aatgagttca ttttccatcg ttttgttccg ggcaggcgct ggcgttcttt aatctgcctt
      781 atggcagcag caagttcaga ggcaggaacg atctgttccc cagcagtcac cgcagtaaga
      841 cttccctcct ggtattgctt gcgccagaga agtaactggc tgctacacca tgttgccggg
      901 caacgaggga gaccgtcatt cctggttcaa agctcttctg aacattagcg atcttttcct
      961 gtgcagtatg ccgtctgagt ttctccggtc ctgaggcatc aatcatctgt tttccaatga
     1021 ccagtctaaa aactaatatt aagattatca cttatttaag tgatattggt tgactggaga
     1081 ttcagggggc tagcctatcc gggaaagttc actcactttg aatgaagtaa atattaagta
     1141 tcacattttg tgatgtagcc attattttta taaaaaatca cctgatagga tttatcataa
     1201 gttaatgata agcagagtat tgatagatga gcaagaagaa gttttctgtt gagaatgaag
     1261 atctctctac tgttcgtaca tcacttctta attccaacct tgtccggaag ttgatcatgg
     1321 aaatgtctga tgaagacttc aatcaagtta aggataacct tgaaaaggcc attgagcagc
     1381 gtaatgagtt gaaggattct ttggcaatta tcaaaaagta catggatgac gcaggtgtaa
     1441 cggcggaaga tgtgtatcgt gaatttggca aaaaattaca gacgggcaga aaaaacgatt
     1501 caggagagtc taataagtac aggtatgagt ataacggtca aataatgtac tggagtggac
     1561 aaggtcgtcg gccttctgtt attgcagaag caataaaagc cggaggttct cttgatgatt
     1621 ttcttatatc tcagggcaca tcatcatctt gttctgatta tgagtgatac atctaatcgg
     1681 ttgaatgttg ctgtaatgct cgcttttgat gcttatatgg cagcgttacc gtttattgtt
     1741 ttagttacaa attggtcgtc atggacataa cctgaaatta taactatcat cgggtctttc
     1801 aagttgctta tgcatataac gccagggagg aaaacgagag ttaaaaaatt gaactgttct
     1861 catagagact gtaatttaaa ttgtgtaatt gccggttttt gatatgttca ctccaacaac
     1921 gtagacaagt agattgtgga cgaaaagacg cttaaagtcc ttgcggctga actggctaaa
     1981 ggcctcaaaa ccggggccga tctcaatcag ttttcccgta tgctgacgaa attaactgtc
     2041 gaaacggcgc ttaatgcgga actcggtaac ggctgcagat gttcctgctc tggtgtatgc
     2101 tcagcatcct gttttggtgc ggcgaagaca t
//
LOCUS       DAHBHC010000254         2128 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHBHC010000254 DAHBHC010000000
VERSION     DAHBHC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.259"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..146
                     /locus_tag="M2A35_005360"
     CDS             <1..146
                     /locus_tag="M2A35_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HCB7201490.1"
                     /translation="QKGSGNFSAWVIEACRRRLCSEKRVSSEANKEKSDITELLRKQV
                     RPD"
     gene            204..614
                     /locus_tag="M2A35_005361"
     CDS             204..614
                     /locus_tag="M2A35_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCB7201491.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENV
                     HIVLKNDNTVLLKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFR
                     WVTNIHEQKRYYYTFDNSLLFTESIQKTTQILPR"
     gene            complement(766..939)
                     /locus_tag="M2A35_005362"
     CDS             complement(766..939)
                     /locus_tag="M2A35_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCB7201492.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(1111..1266)
                     /locus_tag="M2A35_005363"
     CDS             complement(1111..1266)
                     /locus_tag="M2A35_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201493.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(1346..1411)
                     /locus_tag="M2A35_005364"
     CDS             complement(1346..1411)
                     /locus_tag="M2A35_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201494.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(1414..1602)
                     /locus_tag="M2A35_005365"
     CDS             complement(1414..1602)
                     /locus_tag="M2A35_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCB7201495.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(1613..1825)
                     /gene="cspI"
                     /locus_tag="M2A35_005366"
     CDS             complement(1613..1825)
                     /gene="cspI"
                     /locus_tag="M2A35_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HCB7201496.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          730 a          404 c          353 g          641 t
ORIGIN      
        1 agcaaaaagg gtccgggaat ttctcagcct gggtcattga agcctgccgc cggagactgt
       61 gctcagaaaa aagagtttct tctgaagcaa acaaagaaaa gagtgacatt actgaattgc
      121 tcagaaaaca ggtcagacca gattgaagca atttagataa tcgtgcagac tacgccccct
      181 catatcacat ggaaggttta tctatggatc aggtagtcat ttttaaacaa atatttgata
      241 aagttcgaaa cgatttaaac tatcaatggt tttattctga gctaaaacgt cacaatgtct
      301 cacattacat ttactattta gccacagaga atgttcatat tgtattaaaa aatgataata
      361 cagtgttatt aaagggccta aaaaacattg tgtctgtcaa attttcaaag gataggcatc
      421 ttatagaaac gacctctaat aagctgaaat ccagagagat cacatttcag gaatacagaa
      481 gaaaccttgc taaagcagga gtttttcggt gggttacaaa tatccacgaa caaaaaagat
      541 attactatac ctttgataat tcattactat ttactgaaag catccagaaa actacacaga
      601 tcttaccacg ctaaaccata acgtccggct tctctcactc ctgagccgga ctgcattggt
      661 ttaataaaaa ccatcaacaa ttgtgattta gatattcgga accattcaaa tataacaaaa
      721 ccccgtaaaa acgaggttta tggataaatt ttattattga atacatcaga ttaaattaat
      781 cttgacatca tagctttcaa gacccgtcat tttttcccgt gcggtaaact gaatactggt
      841 aacttctttc ccggtctttt tcttaagttc aataattttt tttgttatat attcagaaat
      901 atctgcttct gcttttgttt ttaagttttc aatattcatc atttcctctt ttagtctgtt
      961 atgactttcc agttacacag taagtcgatt atatggtgca aacgtgtaaa agataagatg
     1021 aaacatcgca ataatcaaca tacgatagtc taaattttac acaaacagac aaagagaatt
     1081 ttcctgaatt atcaatgcaa tagcatcaaa tcaactcaag agccttattg ctgcttccag
     1141 aatttcttct gaagtaacat gtcgatccgc ggctacataa atgactttat gatctccggt
     1201 cagagatgga aaccctgcgg ccattacagt aaggtgtgtt ttttcgccat ttggatattc
     1261 acgcatgatg gtgttaactc cagtcatcgc tggcactacc actgctggtt cagagttaaa
     1321 aaaaactatg atttttttca tgatgttacc gtagtatgtg agtatccatc gaatagacac
     1381 caagcaaaaa agctcccgaa ggagccttca ttttcacttt tttaaatcca acgacagacg
     1441 gctggcattt aagtattgtg aaatattatc aaatgtaatc atcattgatt tacaaaagat
     1501 acattttgcc ccgaaaggat tcatgtcaga aacatcaaaa gatgatgttc tatactggga
     1561 accatgacaa cacgggcatc taaagtgaat atggtttgta atattgtcta cctcaaagcg
     1621 ccactacatg aacagcggca ggacctttag gtccgttctc aataccaaat tcaacttcct
     1681 gattctcagt taatgttttg aaatcgttgc tctgaattgc tgagaaatgg acaaacacat
     1741 ctttgctgcc atctttcggc gtgatgaaac caaaaccttt ttcagggtta aaccatttca
     1801 ctaaaccagt cattttgtta gacataatta ttaccttttg aagaaattag cccttgggca
     1861 gaatggtccg aaaaaaaata tcagagagaa aaaccaacaa ggaaatctca agaggtacaa
     1921 ataataaaat tataacaatg actgcttcag ataaatttgt aacaaaccag aacaccatta
     1981 acgcatgatt aaccacccat agcaaggatt acttttgtaa agaaaaacac agcaatgaaa
     2041 gaatagcttt atttattaat aaagcgtgtc attctgatta agacctttta tcttaccctt
     2101 aagatttcag gaattttggc tcatggaa
//
LOCUS       DAHBHC010000255         2109 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHBHC010000255 DAHBHC010000000
VERSION     DAHBHC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.260"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            99..782
                     /locus_tag="M2A35_005367"
     CDS             99..782
                     /locus_tag="M2A35_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCB7201497.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGVAN
                     DDWFMPEAA"
     gene            783..1004
                     /locus_tag="M2A35_005368"
     CDS             783..1004
                     /locus_tag="M2A35_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201498.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            1018..1452
                     /locus_tag="M2A35_005369"
     CDS             1018..1452
                     /locus_tag="M2A35_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCB7201499.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            1498..>2109
                     /locus_tag="M2A35_005370"
     CDS             1498..>2109
                     /locus_tag="M2A35_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201500.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSD"
BASE COUNT          464 a          599 c          634 g          412 t
ORIGIN      
        1 tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc accacagggg
       61 ggccctcccg gtgccttctg taagagaagg aggcttttat gtcccgtttt atcctgggtg
      121 actgcgtgcg agttatggcc acgtttccgg acaatgctgt cgatttcatc ctcaccgacc
      181 cgccgtatct cgtcggtttc cgtgaccgtt ccgggcgcac cattgctggc gataaaaccg
      241 acgaatggct gcaaccggcc tgtaatgaga tgtaccgcgt gctgaaaaaa gacgcgttaa
      301 tggtcagctt ctacggctgg aaccgcgtcg atcgctttgt ggccgcctgg aaaaatgcgg
      361 gattcagcgt tgttggtcac ctggtcttca ccaaaaacta cacatcgaag gccgcatatg
      421 tgggctatcg ccacgaatgc gcctacatcc tggcaaaagg ccgtccacgt ctgccacaaa
      481 acccgctgcc ggatgtgctg ggctggaaat attcgggcaa tcgccatcac ccgacggaaa
      541 agcccgttac cagcctgcaa ccgctgattg agagcttcac acacccgaac gcaattgtgc
      601 tggacccgtt tgcaggcagc ggctcaacct gcgttgccgc cctccagtcc ggacgccggt
      661 atatcggtat cgagctgctt gagcagtatc accgtgccgg acagcaacga ctggctgccg
      721 ttcaacgggc catgcagcag ggggtcgcga atgatgactg gtttatgccg gaggctgcgt
      781 aaatgaacta tgccggacac gaaaaacttc gcgccgaagt ggcggaggtg gccaatgcca
      841 tgtgcgacct gcgtacaacc atgaatgaga tggagcagcg gtacagcttt aatgccgaca
      901 ccctgccgga acgtctggtg cgtcagacgc tgtttcgcgc aaaccgcctc ctgatggagg
      961 catataccga aattcttgaa ctggatgcgt gctttaaaga ctgagaagga gacgagaatg
     1021 tacggaacat gcgaaacgct gtgccgggcg ctggcagcaa agtatccggg agacacgcca
     1081 ctgatgctgg ttatctggtc tccggaagag attcaggcac tggctgacgg tatggaaatt
     1141 tctctgaccg atcatgaaat caggaccgtc ctggcgcgca tggaggacat cccggaagac
     1201 cagcggactg aatccggtat ttcttccgcc gcggtgatgg agattatcag caacgtgagc
     1261 gaaaaccgcc aggtgaccgt ccctgctgaa ctgctggcgt ccctgattca gaccgctgaa
     1321 caggcactgt ggaaacgtga gtgggccgcc cgtgataacg gcctcgccgt cccggaatgc
     1381 gttacccgcc gtcaggcggt ggttaatcag gcccgcactc tgctgaaaaa caacacacac
     1441 gaaaacaact gatgttatcg ccgcctgcgg gcggcgatac agggagaacg cttaatcatg
     1501 aacgaaaaca gtacgctgaa cgcactgatt tgtcgtcacg cccgtaacct gctgctggcg
     1561 cagggctggc cggaagagac ggatgttgac cagcgaaatc cgaactatcc gggctggatc
     1621 agcatttacg tgcggctgga tgcgccccgg ctggcgacgt tacttatcaa ccgtcacggc
     1681 ggcgtactgc cgccgctcct ggcctccgcc attcagagac tgaccggaac cggggcggaa
     1741 ctggtactgt ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacgcag
     1801 gtgtctttcc cgtatgccgg agaatggctg accgaagacg aaatcagggc tgttcttgat
     1861 gcggtgcacg atgcggtacg aagcatctgt taccaggtgg cagaagatgc gcggcgtatc
     1921 cgtgcggcgc tgaccaccac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgc
     1981 ctggtcgtga aggaaagcga tcacccctgc tggctcgatg aagatgacga aaacctgccc
     2041 gtggtgctcg atgccatcgt gaaccgggga gcacgttttt cgtcggtgga aatgtacctg
     2101 gtcagcgat
//
LOCUS       DAHBHC010000256         2106 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHBHC010000256 DAHBHC010000000
VERSION     DAHBHC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.261"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(96..1019)
                     /locus_tag="M2A35_005371"
     CDS             complement(96..1019)
                     /locus_tag="M2A35_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCB7201501.1"
                     /translation="MRRVTISVLFLMVALTWGTTWLAMRIAAETIPPVFATGMRFLFA
                     APFLISIAWIRKTPLLFPPGQYLFQFVISVFYFAIPFSLMIYGETYVSSGLASVIFAN
                     MPVAVLVASVIFLNEKTNPLQIAGLTLAIVALAGILLEETKTGTENHWQGIAALVSAV
                     ILHAIIYTRCRKTGFTVSVITFNALPCFLAGVLLSVTGWLFEKPQVSSFSLHSILATL
                     YLGGFAGVFGILCYFALQQKATAFQASLVFLVFPLIAVTLENYLYGDAISAQSMLFII
                     PLVAGIFITLFCQRAPVSSRERDGSQQKRNA"
     gene            complement(1298..2089)
                     /locus_tag="M2A35_005372"
     CDS             complement(1298..2089)
                     /locus_tag="M2A35_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCB7201502.1"
                     /translation="MSRRYLSENEVQLLLDTAQHHSLRNYCMIYLAFIHGLRASELLS
                     LQLTDYDPLASTLSIRRIKGGLSTIHPLSPEEGHIISLWLQERSRSSSADSPWFFPSR
                     NHTHITRQRFWQIIRLYGVMAGLTVRVHPHMLRHACGFALAERGNDTRLIQDYLGHRN
                     IRHTVRYTATSPSRFRQAWSSPPRHTLIPEKTRPETSVTQTYHPPHGEDTATGLSPPD
                     LSLIHHEYGVMIKNIWQELSSIQHTLRRLDLRAAAGQQCVLNERC"
BASE COUNT          544 a          492 c          573 g          497 t
ORIGIN      
        1 acaggctatg ttgccacata ttattttctg tatatcggtt cagggatgac cattgcttat
       61 taccaggctg ttcattccga aaccgctttc cgttctcagg cattcctttt ttgttgtgag
      121 ccgtcccttt cccggctgct tacaggcgct ctctgacaga acagtgtgat aaatatcccg
      181 gcgactaacg ggataataaa aagcattgat tgtgcagaaa tcgcatcccc gtataaataa
      241 ttttccagcg ttaccgcaat cagcgggaaa acaaggaaaa caagagacgc ctgaaatgca
      301 gtggcttttt gctgaagtgc aaaatagcat aatattccga aaacgccggc aaacccaccc
      361 agatacaggg tggcaaggat tgagtgtaat gaaaaagagg atacctgtgg tttttcgaat
      421 aaccatccgg tgacagaaag gagcacccct gccagaaaac acgggagtgc attaaacgtt
      481 atcacagaga cagtgaagcc tgttttcctg caccgggtgt atattatggc gtggagtatc
      541 acggcagaga caagcgcagc gatcccctgc cagtgattct ctgtacccgt tttcgtttct
      601 tcgagaagga tccccgccag tgccacgatg gcaagagtta atcctgcaat ctgtagtgga
      661 ttcgtttttt catttaaaaa aatgactgaa gcaacaagga cagccacagg catatttgca
      721 aatataacag aagcaagtcc ggaactgacg tatgtttcac cataaatcat taatgagaag
      781 ggaatggcaa aataaaatac agaaatcacg aactggaata aatattgtcc gggtggaaat
      841 aaaagcggtg tttttcttat ccatgcaatg cttatcagaa atggtgcagc aaacaaaaat
      901 cgcattccgg ttgcaaatac cggagggatg gtttcagcgg ctatccgcat ggccagccat
      961 gtggttcccc atgtcagcgc aaccatcagg aataatacgg aaatcgtcac tctgcgcata
     1021 aaattctccc ggcaaaaacg ttagtgtaac gttacatata agtgaatcct atgattcata
     1081 tgtgtcatat gccatgttct tgcctgccgg gggcggcttc ccgggacagg agcacgcccg
     1141 gtcaggcatt tccggtactg atgcgggccc tgacgaccgc ctgcagcatg agagccgcgg
     1201 cagacagggc atgatgagac tccctgtcag ttgctgtcat caggagtctg tccagcagac
     1261 tttcaggctc tccctttgtg tcaacgcgtt aagtttttca gcaccgttcg ttcagcacgc
     1321 actgctgccc ggccgcggcc cgtaaatcaa gtcttctcag ggtatgctgt atactgctca
     1381 gctcctgcca gatatttttt atcatgacgc cgtattcatg gtgtatcagt gaaaggtcag
     1441 gcggggaaag accggtggcc gtgtcctcac cgtgaggagg atgatatgtc tgcgtaacgg
     1501 aggtctccgg acgtgttttt tctggtatca gggtgtgacg gggaggagat gaccatgcct
     1561 gccggaagcg tgacggactg gttgcggtgt aacggacagt atgccggatg ttccggtgtc
     1621 cgagataatc ctgaatcaga cgggtgtcgt tgccgcgttc agcaagggca aagccacagg
     1681 catggcggag catatggggg tgtaccctga cggtcagccc ggccatgaca ccgtagagac
     1741 ggataatctg ccagaaacgc tgacgggtga tgtgtgtgtg gtttcgtgaa gggaaaaacc
     1801 agggggagtc tgcggacgaa gagcgtgaac gctcctgtaa ccacagactg atgatgtgtc
     1861 cctcttccgg gctgagcgga tggatggtgg acagccctcc cttgatacgc cggatggaga
     1921 gtgtggacgc cagcgggtca taatccgtga gctgcagcga gagcagctca gaagcacgaa
     1981 gcccgtgaat gaaggccaga taaatcatgc agtaattacg cagggagtgg tgttgtgcgg
     2041 tatccaggag cagctggact tcattttcgg acagatagcg tcgcgacacg ttatcatctc
     2101 ctgtca
//
LOCUS       DAHBHC010000257         2094 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHBHC010000257 DAHBHC010000000
VERSION     DAHBHC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.262"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1300
                     /locus_tag="M2A35_005373"
     CDS             <1..1300
                     /locus_tag="M2A35_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201503.1"
                     /translation="DDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAK
                     RVWRRERDLTGWMSLSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIET
                     DNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWL
                     AQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQL
                     NEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYP
                     LNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKK
                     GEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAY
                     TYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            1312..1497
                     /locus_tag="M2A35_005374"
     CDS             1312..1497
                     /locus_tag="M2A35_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201504.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKNLST"
     gene            1497..1706
                     /locus_tag="M2A35_005375"
     CDS             1497..1706
                     /locus_tag="M2A35_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201505.1"
                     /translation="MNEAKEKDLGTYKKSTLKTEKITRGLFSNDEITLIYFSEYSKRI
                     VQEVFVFNVEDKKVKLKGYRYDSIS"
     gene            complement(1728..1847)
                     /locus_tag="M2A35_005376"
                     /pseudo
     CDS             complement(1728..1847)
                     /locus_tag="M2A35_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          597 a          411 c          595 g          491 t
ORIGIN      
        1 ggacgacgag cggacccacc actaccacta cgacagccag caccgtctgg tgttctacac
       61 gcggatacag catggcgagc cactggtcga gagccgctac ctctacgacc cgctgggacg
      121 gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc
      181 gcgtaaaccg gagatgacgt ggtacgggtg ggacggcgac cggctgacga cggtacagac
      241 cgacaccacg cgtatccaga cggtatatca gccggggagc ttcgcgccgc tcatccgcat
      301 cgaaacagac aacggcgagc gggagaaagc gcagcgccgc agcctggcgg agaagctgca
      361 gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg gctgaactgg tgcggctgct
      421 ggacaggctg gaggaagaaa tccgggcaga ccgcgtgagc agtgaaagcc gggcgtggct
      481 tgcgcagtgc gggctgacgg tggagcaact ggccagacag gtggagccgg aatacacacc
      541 ggcgcgaaaa gttcattttt accactgcga ccaccggggc ctgccgctgg cgctcatcag
      601 cgaagacggc aatacggcgt ggcgcgggga gtatgatgaa tggggcaacc agcttaatga
      661 agagaacccg tattacctgc accagccata ccgtctgccg gggcagcagc atgatgagga
      721 atcagggctg tactataacc ggaaccggta ctatgacccg ctacagggga ggtatattac
      781 acaagacccc attgggctgg cggggggatg gaacctgtat aattacccac tgaatccgat
      841 aataaggatg gatcctttgg gtttgtataa tttatatcaa ttattatatg atgtttggca
      901 tgatgattca tatggaacat catcaattga tattactggc agtggagatc taatatcatt
      961 aggtggtcat gcaggacttg gcgttgcgtt tgctaaaaag aaaggtgaaa tgttatctga
     1021 tatttgtatt tatgctacag catgcggaca tgcaggaatt ggtggtggga taaatgcggc
     1081 tatcacatat tctgagacca agtctttacc tacatcggga gtcagcaatt cagtaggtgt
     1141 aacggttggc ggcggagttg gggggcattt tgcgtatact tatgtagtgg atgttgataa
     1201 tccagaatca tcgacagaat ctgttggtat cggtgcaggt gttgacgctt cagttatgac
     1261 tctggcttgt agaacgtggc aagaatgctg ggtcaattaa caggaggaaa tatgaaaaaa
     1321 tatatatttt tagttttatt tttattgtgt ggttgttctc cagtggacat agagccagct
     1381 agacaagaag ttaatatctt tcgtggttta tatcagacag ggaaatacag tgatatatat
     1441 aaaatcactt caagtgattt tcagactgcc acatctgaaa aaaatttatc gacataatga
     1501 acgaagcaaa agaaaaagat cttgggactt ataaaaaatc aacccttaaa actgaaaaaa
     1561 tcactcgcgg attattttca aacgatgaaa ttacattgat atatttctca gaatattcaa
     1621 aaagaattgt ccaagaggtt tttgtgttta atgttgaaga caaaaaagta aaattaaaag
     1681 gttataggta tgattcgatt agttaaattt tttgatgatg acttcaatta gtagaacatg
     1741 tatttttcga taaaagcacc gatgactttt tcatgaagtt ctacagagcg cgagaaacag
     1801 atggttctgc gagtcagacg tttgattcag gttctcaggg gcagattcag ggaaagatca
     1861 cagggaaaga tcatcggtgt gccgtaggca caaagatgca aaacgggacg ttttgcatgt
     1921 aataagcata agcttgtgaa gagatggtgg caggcccacc gggatctgct gtcggaagtc
     1981 ggtcccaaac aaccccggct gctgtggtaa agagccatgg tagtgagcgc agggggaacg
     2041 atcagacgag atctgatcct atgtgagatt agggaaagat catcaaaagt tcac
//
LOCUS       DAHBHC010000258         2071 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHBHC010000258 DAHBHC010000000
VERSION     DAHBHC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.263"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..856
                     /locus_tag="M2A35_005377"
     CDS             <1..856
                     /locus_tag="M2A35_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7201506.1"
                     /translation="NTTLGETWEAKIGERPDAELMVERKEHYSAPVPDRVAYLTAGID
                     SQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYTRRNGAEMSV
                     SRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIG
                     TDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKK
                     ILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLADYA
                     RALSGEDE"
     gene            853..1059
                     /locus_tag="M2A35_005378"
     CDS             853..1059
                     /locus_tag="M2A35_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCB7201507.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTLRRRGPAGFYV"
     gene            1056..>2071
                     /locus_tag="M2A35_005379"
     CDS             1056..>2071
                     /locus_tag="M2A35_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCB7201508.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESGLDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSL"
BASE COUNT          493 a          531 c          643 g          404 t
ORIGIN      
        1 gaacaccacg ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgccgagct
       61 gatggtcgag cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac
      121 cgccggtatc gactcccagc tggaccgcta cgaaatgcgt gtatggggat gggggccggg
      181 tgaggaaagc tggctgattg accggcagat tattatgggt cgccacgacg atgaacagac
      241 gctgctgcgt gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat
      301 gtcggtatcc cgtatctgct gggatactgg cgggattgac ccgaccattg tgtatgaacg
      361 ctcgaaaaaa catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa
      421 gccggtggcc agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg
      481 tacggatacc gcgaaagagc aaatttataa ccgcttcaca ctgacgccgg aaggggatga
      541 accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc
      601 gcagcagctg actgctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact
      661 gtgggacagc aaaaagcgac gcaatgaggc actcgactgc ttcgtttatg cgctggcggc
      721 gctgcgcatc agtatttccc gctggcaact ggatctcagt gcactgctgg cgagcctgca
      781 ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgcca gagccttatc
      841 cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac
      901 ctgatgacag gtaaacgggt ggcaacagta cagaaagacg gacgaagggt tgagtttacg
      961 gccacttccg tgtctgacct gaaaaaatac attgctgagc tggaagtgca gaccggcatg
     1021 acactgcgac gcaggggacc cgcaggattt tatgtatgaa aacgtccacc attcccaccc
     1081 ttctggggcc ggacggcatg acatcgctgc gtgaatatgc cggttatcac ggcggtggca
     1141 gcggatttgg tgggcagttg cgggcgtgga acccaccggg tgaaagtgtg gatgcagccc
     1201 tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct
     1261 atgccgccaa cgccatccag ctgcatcagg atcatattgt cgggtctttt ttccggctca
     1321 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     1381 gcgaggttga agcggcatgg aaagaatttg ccgaggatga ctgctgttgc attgacgttg
     1441 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcat gcctttaacg
     1501 gtgaactgtt cgttcaggcc acctgggata ccagcccgtc gcgactgttc cggacacagt
     1561 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     1621 gccgtgccgg tgtgcagatt aatgacagcg gcgcggcgct gggatattac gtcagcgagg
     1681 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     1741 gtcgcgcctc gttcattcac gtctttgaac ccgtggagga cgggcagacc cgcggtgcaa
     1801 atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc
     1861 tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat tgaaagtggg ctggatacgc
     1921 agtcagcgat ggattttatt cttggcgcga acagtcagga gcagcgggaa aggctgaccg
     1981 gctggattgg tgaaattgcc gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa
     2041 aagtgccgca cctgatgccg ggtgactcac t
//
LOCUS       DAHBHC010000259         2058 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHBHC010000259 DAHBHC010000000
VERSION     DAHBHC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.264"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..171)
                     /locus_tag="M2A35_005380"
     CDS             complement(<1..171)
                     /locus_tag="M2A35_005380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201509.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAE"
     gene            308..>2058
                     /locus_tag="M2A35_005381"
     CDS             308..>2058
                     /locus_tag="M2A35_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCB7201510.1"
                     /translation="MEDASLTTKGVVKLSSAVDSTSESLAATPKAVKAANDNANSRVP
                     SNRKVNGKALTADITLTPKDIGTLNSVTMSFSGGAGWFKLATVTMPQASSIVYIALIG
                     GAGYNVGSPHQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTYDIYV
                     EIGNYATRVNIHWDCTANATVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTPSDIG
                     ALPTTGGTVSGPLSVTGGLTGSLNGNASTATKLQTARSIGGVVFDGSANINLPGVNTT
                     GNQNTTGNAATATKLQTARKISGVPFDGSTDITLTAAHVAAFARRATDTYADADGGVP
                     WNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEDDW
                     AEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSGVIPD
                     MRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGA
                     HTHSVSGTAASAGAHTHSMTFVSGGSSGAPGSGSPDYSKYSVNTSSAGAHTHSVSGTA
                     ASAGAHAHTVGIGAHTHS"
BASE COUNT          544 a          506 c          524 g          484 t
ORIGIN      
        1 ttccgcagcg aagacatccg agacgaacgc gaaagcgtcg gaaaccagcg cagaatcctc
       61 aaaaacggca gccgcatcgt ccgccagttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacca gacaggcgtc agcagcgaag ggcagcgcca cgacggcatc
      181 cacgaaagca acagaggcgg caggcagtgc gacggcagca tctcagagca aaactactgc
      241 tgaatccgct gcgacccgtg cggaagctgc tgctgatcgt gctgaagaga ttgccggtgc
      301 agttgcgatg gaagacgcaa gccttacaac taaaggtgtt gtgaaactta gcagtgctgt
      361 tgatagcacc agtgaatcgc tggccgcaac gccaaaagca gttaaagcag ccaatgacaa
      421 tgcgaatagc agggtgccat ctaaccgaaa agttaacgga aaagcattga ctgcggatat
      481 aacattaacg ccgaaagata ttggtacttt aaattcagta acgatgtctt tctctggcgg
      541 ggctgggtgg ttcaaactgg ctacggttac catgccacaa gcgagttcca tcgtttacat
      601 cgcattgatt ggtggcgctg gttacaacgt cggttcccca catcaggcag gcatttcaga
      661 actggttcta cgagcaggca atggaaaccc caaaggaatt accggggctt tgtggaagcg
      721 tacagccgtc ggattaacga atttcgcctg gatcaacaca tccggcgata catatgatat
      781 ttacgttgag attggcaatt atgcgactcg tgtaaatatc cattgggatt gtactgcaaa
      841 tgcgacagtt tctatttata catcgccaac atattcagcg agtaagcctt ccagcgttac
      901 cgatggtgtt gtttatacga tgtatagctc acatcagaaa cctacaccat cagatattgg
      961 agcactgcca acaaccggag gaacagtttc aggtccgttg tctgttacag gtggattaac
     1021 tggttcattg aatggtaatg caagtacagc cacgaaattg cagacggcaa gatctatcgg
     1081 tggagttgtt ttcgacggtt ctgcaaatat taaccttcca ggtgtaaaca ctacgggtaa
     1141 tcagaacacc actggtaatg ctgcaactgc tacaaaactt cagactgcaa gaaaaatatc
     1201 cggtgttcca tttgatggtt ctactgatat cactttaacc gccgcgcatg tggctgcttt
     1261 tgccagaaga gcaacggata catatgccga tgcggatggt ggcgttccct ggaatgccga
     1321 atcaggtgct tacaatgtca cccgctctgg cgacagctat attctggtta acttctatac
     1381 cggagtcgga agttgccgga ccttgcagat gaaggcgcat tacagaaatg gtggtctgtt
     1441 ctaccgttct tcaagagacg gttatggttt tgaggatgac tgggcagaag tttatacctc
     1501 gaaaaatctt ccaccagaaa gctacccagt cggcgcacca atcccgtggc catcagatac
     1561 cgttccgtct ggctatgccc tgatgcaggg gcagactttt gacaaatctg catacccgaa
     1621 acttgcagtg gcttatccgt caggcgttat tcctgatatg cgtggctgga cgattaaggg
     1681 caagcccgcc agtggtcgtg ccgtattgtc tcaggaacag gacggcatta aatcgcacac
     1741 ccacagcgcc agcgcatcca gtacggattt ggggacgaaa accacatcat cgtttgatta
     1801 cggcactaaa tccacgaata acactggtgc acatacccat agtgttagcg gtacggctgc
     1861 ttcagccggt gcacataccc attcgatgac atttgtttca ggtggttcca gtggtgctcc
     1921 gggaagtgga tcacctgatt attctaaata cagtgttaac acttcttctg caggcgctca
     1981 tacgcactct gtatcgggta ctgctgcaag cgcaggtgca cacgcacata ctgtcggtat
     2041 tggtgctcat acgcactc
//
LOCUS       DAHBHC010000260         2053 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHBHC010000260 DAHBHC010000000
VERSION     DAHBHC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.265"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..444
                     /gene="traK"
                     /locus_tag="M2A35_005382"
     CDS             <1..444
                     /gene="traK"
                     /locus_tag="M2A35_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCB7201511.1"
                     /translation="ERGLNLSIQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYES
                     LLVTISQAVRGGKLPAGWYQIPVTKETLQAPAGLSSVADAVWTGNHLKMVRFSVENKT
                     LSALNIRESDFWQPGTRAVMFSQPASQLLAGARMDVYVIRDGEGN"
     gene            444..1871
                     /locus_tag="M2A35_005383"
     CDS             444..1871
                     /locus_tag="M2A35_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCB7201512.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERVSQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVSVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            1861..>2053
                     /locus_tag="M2A35_005384"
     CDS             1861..>2053
                     /locus_tag="M2A35_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCB7201513.1"
                     /translation="MANNMSLRQICHATRYVVAQGLCWLFWCLKYAVILPLATMALLA
                     LLVLWKDNTTPGKLLAQEIE"
BASE COUNT          461 a          535 c          650 g          407 t
ORIGIN      
        1 gagcggggtc tgaacctttc cattcaggcc gttccccgtg aaggcgcggg gcgtaccatt
       61 cagctggtca gtgacctgcg cggaaccgga gaagaagccg gagcgtggga aacgtccacg
      121 ccttacgaat ccctgcttgt gaccatcagc caggccgtcc gtggcggtaa attacccgcc
      181 ggctggtatc agattcctgt aacaaaagaa accctgcagg ccccggcggg gctgtcttca
      241 gtggcagatg ccgtatggac ggggaatcac ctgaagatgg tccgcttttc cgtggaaaat
      301 aaaacgctgt ctgccctgaa tatccgggaa agtgatttct ggcagccggg aacccgtgcc
      361 gtgatgttca gccagcctgc cagccagtta ctggcaggtg cgcgcatgga tgtgtatgtc
      421 atccgtgacg gggagggcaa ctgatggcca gtatcaatac cattgtgaaa cgcaagcagt
      481 acctgtggct ggggattgtg gttgtcggtg cagcctccgc gattggtggt gcactgtatc
      541 tgtctgatgt ggacatgtcc ggtaacggtg aggccgtggc tgaacaggaa cccgtgccgg
      601 atatgaccgg tgtggtggat acgacctttg atgacaaggt gcgtcagcat gccaccacag
      661 agatgcaggt gacggcagcg cagatgcaga agcagtatga ggaaatccgt cgcgagctgg
      721 atgtcctgaa caaacagcgc ggtgatgacc agcgtcgtat tgaaaagctg ggacaggaca
      781 atgccgccct ggcagagcag gtgaaagccc tgggtgcaaa tcctgtcacc gcgaccggtg
      841 agcctgtacc gcagatgcct gcctcaccgc ccggcccgga aggcgaacca cagccaggaa
      901 acacccccgt atccttcccg ccgcagggca gcgttgctgt tccaccgccg acggcgtttt
      961 atcccgggaa tggtgtcacg ccaccaccac aggtgacgta ccagtctgtg ccggtgccta
     1021 accggataca gcgtaaggtg tttacccgta atgagggaaa acagggacca tcactgccgt
     1081 acattccgtc aggaagtttt gcgaaagcca tgctgattga aggggcggat gccaatgcgt
     1141 cggtcaccgg taatgaatcc acggtgccga tgcagctgcg tatcaccggc ctggtggaaa
     1201 tgccgaacag caaaacgtat gacgcaacgg gatgttttgt ggggctggaa gcctgggggg
     1261 atgtctccag tgagcgtgcc attgtacgca cccgcaatat cagctgcctg aaggacggca
     1321 aaaccattga tatgccgatt aaggggcatg tcagcttccg gggtaaaaac ggtatcaagg
     1381 gcgaagtggt gatgcgtaac ggcaaaatcc tcggctgggc atggggcgcg ggatttgttg
     1441 acggtatcgg tcagggaatg gagcgcgtct cccagccggc tgtcgggctg ggtgccaccg
     1501 ctgcttacgg ggccggcgat gtccttaaaa tgggtatcgg tggcggcgca tcgaaagccg
     1561 cacagacgct cagtgactac tacatcaaac gtgccgaaca gtatcacccg gtgataccga
     1621 ttggtgcggg caacgaagtg accgtggtgt tccaggacgg cttccagctg aaaaccgtgg
     1681 aagagatggc gctggaacgc acgcagagca gagcggaaga agacaatccg gaaagtccgg
     1741 tttctgttcc gccatcggct gaaagtcatc ttaacggctt taatactgac cagatgctga
     1801 agcagctggg caacctgaat ccgcagcagt ttatgtccgg cagccaggga gggggcaacg
     1861 atggcaaata atatgtcact ccgtcagata tgccatgcca cccggtatgt tgttgcccag
     1921 ggcctgtgtt ggttgttctg gtgtctgaaa tacgccgtca ttctgccgct ggccacaatg
     1981 gctctgctgg cgttactggt tttatggaag gacaacacca caccggggaa attgctggct
     2041 caggagatcg agt
//
LOCUS       DAHBHC010000261         2025 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHBHC010000261 DAHBHC010000000
VERSION     DAHBHC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.266"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>2025)
                     /locus_tag="M2A35_005385"
     CDS             complement(<1..>2025)
                     /locus_tag="M2A35_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7201514.1"
                     /translation="TREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLER
                     ERQALARVTRARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMS
                     RVQALTRIDQNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALP
                     GVLSMATAGGMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGET
                     MKYAGPVAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALK
                     ELGVAVSDANGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAA
                     GDGSLPKLRKELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRS
                     VTQWLTRVVSRVTALAQAHPTLTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLR
                     LGFSLLTGSMNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKY
                     WDPIRAFFAGVFSGIMERLIPLRETFEQFGPVFDAIGSGISQVFNWFKSLLSPMESSK
                     ETLDKCTSAGEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIED
                     AQRAAILQDKVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGT"
BASE COUNT          420 a          644 c          511 g          450 t
ORIGIN      
        1 gtaccggtat tgctgtctgt gagcggtttg ttgtctttgg gtttatcacc tccggtgcca
       61 ttgccatttt ccacaggctt cggcggattg attttcgcca ggtccccctg aagcaaggca
      121 accttgtcct gaagaatggc cgcacgctgt gcgtcttcga ttttctttct cgccctttcc
      181 gcttcatccg gaagaacacc gagtttttca agtatccacg ccagcgtatc cagcaacatt
      241 tttgcaggcg tcagaacaag ctgtaacgcg ccaccaagaa cgttaccgaa tatctcgcca
      301 gcactggtac atttatccag cgtttccttg ctggactcca tcggtgacag cagcgattta
      361 aaccagttaa acacctggct gatcccgctt ccgattgcgt caaaaacagg accaaactgt
      421 tcaaaggttt cgcgcaacgg gatcagcctt tccataatcc cgctgaacac cccggcaaaa
      481 aatgccctga tgggatccca gtatttccag ataagaacgg cagctccggc aagcgcagcc
      541 acgataagac caaccggact gaacagcgcc ccgatagcac ctcccagcaa agaaacggaa
      601 cccgtcacca ttccccatag tgctggcagg accctgacag cattcattga tccggtcagg
      661 agagaaaaac caagacgcag ttttgccagc gggccagcaa gcacaccaat agccagcgac
      721 aacgagccaa ccgttgcagt cattgccagc aacgcaccgc ctgctatcag tagctggcgc
      781 gtcagtgtcg gatgggcctg cgccagcgcc gtcacccttg ataccacccg tgtgagccac
      841 tgcgtgacag aacgcagcgg accgtcaata agatctgcaa tgcggatgcg caacccttcc
      901 catgcactgc cgagtgattt cagatcgccg tcaaggttgt tggccataac ctttgctgtg
      961 cgttcagcct caccgcgtgc gccttcaagt tcttttctca gtttaggtaa ggaaccgtca
     1021 cccgctgcat caacgagcgc cataaacgat gtgaaagcct cttctccggc aatgtcctta
     1081 aagaacgata cccggtcaac ttccccgtat ttgcgggtgg ctttataaag gtcggccagc
     1141 acatcctcca tcgggcgcat tttgccgttc gcgtcagaga ctgccacacc aagctctttc
     1201 agcgcctctg ctgccgcctt tggcggtgat gccagacgag ccaggctggc acgcattgcc
     1261 gtcccggcat cacttcccct gatacccata ttcgccagca cgcccgccat cgctgcggcc
     1321 tgctccagcg atattcccag cttgcccgcc accggacctg catatttcat ggtttcgccc
     1381 agtgcgcgaa ggtcagtgtt ggtacgggta aacgctgcgg tgagcgtgtc accgacccgg
     1441 tccatctggt cggcagaaag gccgaactgc gtcaggatgt ttgagccaat atccgccgtc
     1501 tcgccgagat ccataccgcc agccgttgcc atgctcagca caccgggaag cgcagcctga
     1561 atggcctgtg gtgtgaagcc agccattgca agaaatgcct gtccactggc ggcatctcca
     1621 gcggtgaact gcgtttcaga gccaagtttt aacgcctgct cacgcagcgc cttaaactgt
     1681 gggctgttct ggtcgattcg cgtcagcgcc tgaacgcggg acatctcttt cccgaacccg
     1741 atcgcaggct gcaaaaaacg cccggcagca tagccgcccg ccgctgccgc accaattgcc
     1801 agcgcaccac ctgttttcag ttttcccgcg gtttcctgcg cgcgcgaata ccgctcacgc
     1861 gcccgcgtta cacgcgcaag cgcctgccgt tcgcgttcaa gctggttgtt gtactgttcg
     1921 gtgcgtctga tggcctgctg gatggtgtta tcgctgcctg tcagggaaat gccgtggcgt
     1981 ttcagttctc cgccaagttc ccgcattttc tgaatttccc gtgtg
//
LOCUS       DAHBHC010000262         2004 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHBHC010000262 DAHBHC010000000
VERSION     DAHBHC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.267"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..345
                     /locus_tag="M2A35_005386"
     CDS             <1..345
                     /locus_tag="M2A35_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCB7201515.1"
                     /translation="LSVMATNGMRTSHHIANTIIEHRHDEAWLRQHIHNNAHYASLMA
                     VIREKEETLKQRVIRALAGFSGYGRVMVVGGGAEIVAPAIREACGVNATFIADGVPQF
                     ALVNGLYAMDKE"
     gene            350..661
                     /locus_tag="M2A35_005387"
     CDS             350..661
                     /locus_tag="M2A35_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCB7201516.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVSQPVPTAQITSQMAGNIRALFP
                     E"
     gene            722..1246
                     /locus_tag="M2A35_005388"
     CDS             722..1246
                     /locus_tag="M2A35_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201517.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     IHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQLNKAVQNTFVKKLMPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPLHDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QAQEELTQHKGGNQ"
     gene            1243..1832
                     /locus_tag="M2A35_005389"
                     /pseudo
     CDS             1243..1832
                     /locus_tag="M2A35_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT          555 a          495 c          512 g          442 t
ORIGIN      
        1 ctgtcggtga tggccaccaa cggtatgcgt accagtcacc acatcgctaa taccattatc
       61 gaacatcgcc acgatgaagc ctggctgcgc cagcacatcc acaataacgc acattacgcc
      121 agcctgatgg cagttattcg tgaaaaggag gaaacactga aacaacgcgt gatccgcgcg
      181 ctggcgggtt tttcgggtta cgggcgggtg atggttgtag gtggaggagc ggagattgtg
      241 gcacccgcta tccgcgaagc ctgcggagtt aatgcgactt tcatcgcgga cggggtgcca
      301 cagtttgctc tggttaatgg gctgtacgca atggacaagg agtaaatcaa tgacgacacc
      361 aaccagacga ataagtttct atctgaagcc cgccgccgtc aagaacgaag gcgaagcatg
      421 cgcctggctg gacagcctta caccagaagc ccgcaaaagc ggccaacgcg tggcttttct
      481 ggccgggctg gcacttctga aaatgaatcc agcagaggct taccgactgg ctgcatgggc
      541 tgacgatgag gcgttatcgg tgacacaaac caggacagaa cgccccgtgt cacagccagt
      601 accaaccgca cagataacca gtcagatggc agggaatatc cgggcattat ttcctgaatg
      661 acgaatttga cggcttttaa ttttatcggg gtgcactgtc gccccgcacg aggaaaacac
      721 gatgaaaaaa tacgaataca tgctaatgaa agaagcactg cgggctggtc tggctccctg
      781 tcctgtatcc gtagaagctg ttgttgggaa cacgattttc cgaacgctgc gaaatcgcgt
      841 atccgtgaag atgattcacc aaccacccaa atctaccagc atgatctatc tgccgctgcc
      901 cgaggctatc aaacttatgc gttcgctgaa atttgcgatt tggatgcaga cacaactcaa
      961 caaagcggtg caaaacacct tcgttaagaa actgatgccg gagcttgccg ccagatgtgc
     1021 aaaatgtacc agaccagcac ttcaatttga tctgtcattg attcaacaag ctacggaggc
     1081 agaactgtac cgcctgtcac ctctccatga tgctcacacg gaagcctcag aaatgttccg
     1141 gctcatgttt caatttaatt ttgccgttcg acgcacacgc gttcaggtca atcgcatgat
     1201 gcaggcgcag gaagagctga cgcaacacaa aggaggcaac cagtgagcaa gattgactat
     1261 caggcattac gcgaacgtta ttcacctgcg ccagtaccga aatgccctat ttgcggtgag
     1321 gaaatgtcaa ttcagcgaat atctggagca caggttgttt atgcctgctc tggttatggt
     1381 gatgatggag atttcaaaat tggtcgaact cttgccgacg aacattatga aaaatcacgc
     1441 gtaacagtgt tggatgtcgg ggatcctgaa gtattggcgc tacttgattg gctggaaact
     1501 aaagacaacc gaatcgctga actggaaaaa atcgccactg actatgcact taaattccag
     1561 aaagcacagg acgcattaaa gtatgccgct ttgctgcata gccggacggc gcaacaaaca
     1621 aataattttg cagtattgct tccggacata agcgaatatt tcattaataa cgtatttcag
     1681 cccttgcgat acgagcggga tgttgaaaga gccatcataa aggctggcgg aaaagcattg
     1741 tggcaggaga agcacgcgaa agaacgcatc agctaagtga tgtaaatagt ggatgcttat
     1801 gcccactgac agaaggtaaa aagaacacct gatttcctct caaactaggg cgataatcgc
     1861 gcatcgccct gctgcacaat agcgcacaaa tttgcacaat ttttttgaac gactttttgc
     1921 ccttccggcc cccgtggcgg ctggatccgt caaggatccg tgcgtgcaca aaaaaacgcg
     1981 ttttttctgc gcgcaggtga cggg
//
LOCUS       DAHBHC010000263         1929 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHBHC010000263 DAHBHC010000000
VERSION     DAHBHC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.268"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..588
                     /locus_tag="M2A35_005390"
     CDS             <1..588
                     /locus_tag="M2A35_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCB7201518.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            560..943
                     /locus_tag="M2A35_005391"
     CDS             560..943
                     /locus_tag="M2A35_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HCB7201519.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1048..1890
                     /locus_tag="M2A35_005392"
     CDS             1048..1890
                     /locus_tag="M2A35_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCB7201520.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMTLND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
BASE COUNT          685 a          282 c          415 g          547 t
ORIGIN      
        1 tatatcactc aggatccgat tggactgaag gggggatgga acctgtatgg atatcaattg
       61 aatccgatat cagacatcga ccccctgggt ttatctatgt gggaggatgc aaaatcgggg
      121 gcatgtacta atggtctttg cggcacacta tccgctatga taggtccaga taaatttgat
      181 tctatagata gcaccgcata tgacgcctta aataaaataa atagccaatc tatttgcgaa
      241 gataaagagt tcgctggttt aatatgtaag gataatagtg gcagatattt ctcaacagca
      301 cctaaccgag gagaaagaaa aggatcatat ccattcaata gcccttgccc taatggtact
      361 gagaaagtat cagcttatca tactcatggt gcagatagtc atggagaata ttgggacgaa
      421 atattttcag gtaaagatga gaaaatagtt aaaagtaaag ataacaatat caagtcattt
      481 tatttaggta cgcccagtgg taattttaaa gcaatagata accacgggaa ggaaataaca
      541 aacagaaaag gattacctaa tgtctgcaga gttcatggta atatgtaaaa aaatattgtt
      601 taggaactgt gtcattgtat ctttgtttgt ttttacatac aacacatggg cgcagtgtaa
      661 taataatatt aaaataatgc gcaagtatga aagtgaaggt aaatataccg ttagaaattt
      721 ggttaaaaat aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg
      781 acaggatgcc gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt
      841 tgttgaaggt accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg
      901 caaaaatgat gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca
      961 aacatcgtgt gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa
     1021 aagatagcct ttatggaggt tcctgcaatg tcaaatacat accagaaaag aaaggcaagt
     1081 aaagagtatg gtttatataa tcaatgtaag aaactaaatg atgatgaatt atttcgctta
     1141 cttgatgatc acaattcctt gaaaaggatt tcatctgcca gagtattaca gttaagaggt
     1201 gggcaagacg ctgttagatt ggcaattgag ttctgctctg ataaaaatta tatccgtaga
     1261 gatatcggag catttatact cgggcaaata aaaatttgca aaaaatgcga agataatgtt
     1321 tttaatattt tgaacaatat gacattgaat gataagagcg cttgcgttcg agctacggca
     1381 atcgagtcaa cggctcagcg atgcaagaaa aacccaattt attcacctaa aatagtagaa
     1441 caatctcaaa ttactgcttt tgataaatcg actaatgtca gacgtgctac agcatttgct
     1501 atttctgtta tcaatgataa agcaacaatt ccactattga ttaatctgtt aaaagatcca
     1561 aatggagatg tcagaaactg ggccgcattt gcaataaata tcaataaata tgataatagt
     1621 gatattaggg attgttttgt ggagatgctt caggataaaa atgaggaagt ccgtattgaa
     1681 gcaataatcg gactttccta cagaaaagat aaaagggttt tatctgtttt atgcgatgag
     1741 ttaaaaaaaa atactgttta tgatgatatc attgaagctg cgggtgaatt aggtgataaa
     1801 acgctacttc ctgttttaga tactatgttg tacaagtttg atgacaatga aattataact
     1861 tccgctattg ataagctgaa gcgttcatag cgtgagttgc ctatgcacag tgggggattc
     1921 ccgccggca
//
LOCUS       DAHBHC010000264         1892 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHBHC010000264 DAHBHC010000000
VERSION     DAHBHC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.269"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            121..1839
                     /locus_tag="M2A35_005393"
     CDS             121..1839
                     /locus_tag="M2A35_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCB7201521.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRDYRDKFNEFSAYILQMKLAINKL
                     DSELNKLEPSLSAKNHLEKNQGIINSKLTKFNNTIEEKIHSLLKKWADEIKVDRSDYY
                     AKTISVVDSIDNDSQIENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAF
                     SLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGF
                     SLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFS
                     EEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDT
                     DDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQ
                     FNGVEF"
BASE COUNT          669 a          287 c          360 g          576 t
ORIGIN      
        1 gcaggggtcg gcccatttga gcttcagata ggaacgtttg aattcctttc acaaaataca
       61 tcacatactg aacgtgaaca ttcccacttt gatcttaagg ccaaaaagta tgcaggacta
      121 atgattttta gggatagctt gagagtatta ccttatggtc gagtagataa tgatttcttc
      181 cagatagaag aaagacgttc atggaatgca gggcgatatt attggtctaa tagaaggatt
      241 tttggttata ttggaattac tcaatccagt aataaagagc tgaaagataa gtcggggaga
      301 gagggattca taagaaacca ggctgcaaga gaactgaaaa ctattatatc taatttgtta
      361 actgaacttg ctgatagatt ttttggttca cgttctgatg accgtaaaga gcttttagaa
      421 caagttaagc gtgaaaaaga gttaagaaaa tctgctcaac aacaagctcg aaaatcaaca
      481 caaaaaagtt tttcagaagc tttgaagaat cagacaccag ttcttgatgc ttccttggag
      541 gctgttaaaa ggctgaaaac taagcttgat aaaactgatg gttcgttaga tttaaactat
      601 cttaaaatta tagacagtga tcttacaaac ttagatgcgt tgcgcagtga aattaaaacg
      661 cctatcaaac ctccaaaact tggaatgtat gaagagaaat atagagacta cagagataaa
      721 tttaatgagt tctctgcgta tattctacaa atgaagttag caattaataa acttgattct
      781 gaattaaata aacttgagcc ttcattgtca gcgaaaaatc accttgaaaa aaatcaaggt
      841 attattaatt ctaaactaac taagtttaat aacacgatag aggagaagat acattctctt
      901 ttaaaaaaat gggccgatga aataaaggtt gatcgaagtg attattatgc taaaactata
      961 tcagttgttg attcaataga taatgattca caaattgaaa atgtgtttaa tttgctcgat
     1021 agtttatatg ttgagtcagt tgatacccta actttcaaat atcaatcaat aataaaaggt
     1081 ctcgatagat tatttgaagg tataaactta gattcagcat tctcattatc tgaagaagaa
     1141 cgctcatatt ttgaagaaaa agctaaaagt ttaaacgcgc ttgcacagtt aggtattagt
     1201 gttgagataa tatctcatga acttgaagaa atggattcta tggtaaccag aggactaaac
     1261 tctcttccta cttctgtaaa agaacaccct ggtttttcat tggcgttaaa tgctcacaga
     1321 tcgcttactc aacaaatacg tttcttatca cctttgaaaa tatcaggtta tcaatccagg
     1381 cagagaataa ctggaaaaaa tatcatggat tatgtcctga agttctttgg ggagcgtttc
     1441 gaacggcaac gaataactat tgaatttagt gaagagttta agcaaatcgc aataacagat
     1501 ataccatcaa ggatctatcc tgtttttact aatattatca acaatgcaat gtattgggtc
     1561 agtctgtcaa ataataggct cataaagatt ggttttgtga attctttggt tatcatagca
     1621 aattctggtc cggcaattga taccgatgat atcccgcgac tatttgaact attttatagc
     1681 aaaagagcaa atggacatgg ggtaggtctg tatctatgtc gagaaaacct tgctgttgca
     1741 catcataaaa tatggtattc agaacctgat gaaggcgata actatttaat aaaagatggc
     1801 gctaattttg tgatccagtt caatggagtg gagttctaat atgacagtgg caaattataa
     1861 ttctcttgtc cagaaaactt tctgcgaaaa tg
//
LOCUS       DAHBHC010000265         1869 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHBHC010000265 DAHBHC010000000
VERSION     DAHBHC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.270"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            59..490
                     /locus_tag="M2A35_005394"
     CDS             59..490
                     /locus_tag="M2A35_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCB7201522.1"
                     /translation="MKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDDGRGIDT
                     GRALLNWQPHTILDHAHYMELWTGGGLAAGSAREGVNPGILATYGDGGIVKTDEPGIK
                     VPSSLRAISTRSVKRFGEISGNVGTETRPRNIAFNYIVRAA"
     gene            490..1092
                     /locus_tag="M2A35_005395"
     CDS             490..1092
                     /locus_tag="M2A35_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201523.1"
                     /translation="MDKAILNKELITTKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PAYSCLDAPGTHKAGYAICRSADLNSWEYVPDHRGEVVYNTETGESKEITAPGDYPEN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLMQAEKNRLNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(1064..1483)
                     /locus_tag="M2A35_005396"
     CDS             complement(1064..1483)
                     /locus_tag="M2A35_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCB7201524.1"
                     /translation="MKMKWSPSVQGFFSENNSDIPDDAFDIEDALYYELMNGQSTGKI
                     IINNPDNYPVLTEYPAKTQEQEIAEAEGMKSILIEQANEYMNSKQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(1480..>1869)
                     /locus_tag="M2A35_005397"
     CDS             complement(1480..>1869)
                     /locus_tag="M2A35_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201525.1"
                     /translation="CIFINVGLGEAAKRGVGTGENQIPDMASFSGVRDYYGKQLLPGG
                     LILQWLTIPSSAAAKAVTLNNGNYQLSGYKWPQSFGVLFAVFATKVSGSTNEAYAISV
                     NRHSTDVIVTWNARKADDVHILGIGKL"
BASE COUNT          510 a          443 c          428 g          488 t
ORIGIN      
        1 agcgttaccc gttggtgtac ctgttccgtg gccttcagcc acaccgccaa caggctggct
       61 gaaatgcaat ggtgcggctt tttctgctga agaatacccg gaactggcaa aggcttaccc
      121 gaccaataaa ttgcctgatt tacgcggtga atttattcgt ggctgggatg acggtcgcgg
      181 gattgatact ggtcgcgctt tgcttaattg gcagccacac acaattttgg accatgcaca
      241 ctatatggaa ttatggacag ggggcggact cgccgcagga agtgcacggg aaggcgtaaa
      301 cccaggaata ctggctacat acggtgacgg gggaatagtt aaaacagacg aacccggtat
      361 taaggtacct tcctcactac gagctattag tactcgtagt gtaaaacgtt ttggcgaaat
      421 tagtggaaat gtaggtacag aaactcgtcc tcgtaacatc gcttttaatt atatcgtaag
      481 ggctgcctga tggataaagc tatattaaat aaggaactca tcaccacaaa ggcgggagat
      541 attaccgttt ataattatga tggtgaaaca cgggaatata tttccacatc aactgaatat
      601 cttgctgtgg gtgtcggtat ccctgcatat tcctgtttag atgctcctgg cacacataag
      661 gctggttatg caatctgccg ttcggcagat ttaaactcat gggaatatgt gccagaccat
      721 cgcggagaag ttgtctataa caccgaaacg ggagaatcaa aagaaatcac agctccgggt
      781 gattaccctg aaaatacaac cactatcgtc ccgttaacgc catacgataa atgggatggt
      841 gagaaatggg tcacggatac cgaggcacag catagcgccg ctgtagaagc ggcagaagca
      901 cagcgccagt cactgattga tgctgcaatg gcttccatta gtctgattca gctgaaatta
      961 caggccggac ggaagctgat gcaggcagaa aaaaaccggc ttaacgctgt gctggattac
     1021 attgacgcgg tggcggcaac agataccagc accgcgcctg atgtcatctg gcctgaactg
     1081 ccggaggcgt aggccattca atatctgggg cactggaggt atccaccagt tccagtgcgt
     1141 ccaggtaatc cagccacaaa ttatattgcg ccagttcctc acctttcaga cgaccaatag
     1201 cggctttacc aggccattgc ttactgttca tgtattcgtt ggcctgttca ataagtatac
     1261 ttttcattcc ctccgcttca gctatttcct gttcctgtgt cttcgctgga tattcagtaa
     1321 gtacagggta gttatccggg ttattgataa taattttccc cgtactctga ccattcataa
     1381 gttcataata aagagcatct tcaatatcga aagcatcatc gggaatatcg ctgttatttt
     1441 cagagaaaaa tccctgaaca gatggggacc atttcatttt cataatttcc caattcctaa
     1501 aatgtggaca tcatcagcct tacgggcatt ccaggtgaca attacatcgg tagagtgacg
     1561 attaactgag attgcatatg cttcgttagt cgagccagaa acttttgtag caaacacagc
     1621 aaacaggaca ccaaatgact ggggccattt ataccctgac agctgataat taccattatt
     1681 cagtgttaca gctttggctg ctgcacttga tggaatcgtc agccactgaa gtatcaaccc
     1741 tcctggcaaa agttgttttc cgtaataatc cctcacccca ctaaatgaag ccatgtccgg
     1801 tatctgattt tcccctgttc ccaccccccg ttttgccgct tctcccaaac caacgtttat
     1861 gaaaatgca
//
LOCUS       DAHBHC010000266         1864 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHBHC010000266 DAHBHC010000000
VERSION     DAHBHC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.271"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            36..524
                     /locus_tag="M2A35_005398"
     CDS             36..524
                     /locus_tag="M2A35_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCB7201526.1"
                     /translation="MPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLP
                     DLRGEFIRGWDDGRGMDTGRAILSAQGDAIRNIYGEFKTVNTENYSIWESVGSFKGAV
                     VPLNPSTNNSYFSLIRSMVTERIDGAVYPKVIGLDASRIVPTANENRPRNIAFNYIVR
                     AA"
     gene            524..1126
                     /locus_tag="M2A35_005399"
     CDS             524..1126
                     /locus_tag="M2A35_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCB7201527.1"
                     /translation="MNKAVLNNELIAIKAGVITIYNYDGETREYISASTEYLAVGVGI
                     PAYSCLDAPVTSKAGYTICRSVDLKSWEYVSDHRGEIVYNTETGDAKEITALGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAVVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RNLTQAETARLNAALDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1098..1517)
                     /locus_tag="M2A35_005400"
     CDS             complement(1098..1517)
                     /locus_tag="M2A35_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCB7201528.1"
                     /translation="MDRYFYSQKENGFFTDLNKAPSDAVEITTDEWLLLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALVELKKRKLINEANEHMNSRQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDPSSAPDIEWPTPPAVQAR"
     gene            complement(1521..1853)
                     /locus_tag="M2A35_005401"
     CDS             complement(1521..1853)
                     /locus_tag="M2A35_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201529.1"
                     /translation="MGTGENQIPDMSAWKRNPSSNRWRKLPDGTIIQMGISASGPLGS
                     PVNITLPISFSNTNYCVVASYDNARSGVSTMVSFAALPVSPSQFSLMSSVTEQGINPF
                     AYWIAFGD"
BASE COUNT          519 a          422 c          417 g          506 t
ORIGIN      
        1 ctctcgaaaa ccttggttta ggagaaggct cagcattgcc tgttggcgta cctgttccgt
       61 ggccctcagc cactccgcca acaggctggc tgaaatgcaa cggagcagct ttttctgctg
      121 aagaataccc ggagctggca aaggcttacc cgacaaataa attgcctgat ttacgtggtg
      181 agtttattcg tggctgggat gacggacgtg gtatggatac ggggagggca atattatccg
      241 ctcagggcga tgccatacgt aatatctatg gtgagttcaa gactgtaaac accgaaaatt
      301 attcaatatg ggaatcagta ggctcgttta agggggcagt agtgcctttg aacccctcaa
      361 cgaacaatag ttatttctcc ttaatcagaa gtatggtgac agaaagaata gatggcgctg
      421 tttacccaaa agtgattggc cttgatgctt caagaattgt tccaactgca aacgaaaacc
      481 gtcctcgtaa cattgccttt aattatatcg tgagggctgc ctgatgaata aagccgtatt
      541 aaataacgaa ctcattgcca taaaagcggg agtcattacc atttataatt atgatggtga
      601 aacgcgggaa tatatttctg catcaactga atatcttgca gtgggtgtcg gtattccagc
      661 atattcctgt ttagacgctc ctgttacatc taaggctggt tatacaattt gccgttctgt
      721 ggatttaaaa tcatgggaat atgtgtcaga ccatcgcggt gaaatcgtct ataacaccga
      781 aacgggagat gccaaagaaa tcacagcact aggcgactat cccgaaaata caaccactat
      841 cgccccgtta acgccatacg ataaatggga tggtgagaaa tgggtgacgg ataccgaggc
      901 acagcatagc gccgtagtag atgcagcaga agcacagcgc cagtcgctga ttgatacagc
      961 aatggcttct atcagtctga ttcaactgaa attacaggcc ggacggaatc tgacgcaggc
     1021 agaaaccgcc cgacttaacg ctgcgctgga ttacattgac gcggtgacgg caacagatac
     1081 cagcaccgca ccggatgtca tctggcctga actgccggag gcgtaggcca ttcaatatct
     1141 ggagcactgg agggatcaac cagttccagt gcgtccagat aatccagcca taaattatat
     1201 tgcgccagtt cctcaccttt cagacgacca atagccgctt tacctggcca ttgtctgctg
     1261 ttcatgtgct cgttggcttc attaataagt tttctttttt tcaactccac caatgcaata
     1321 aggttttctt ttgataaagg tggttgctct gtcaaaaccg gatatccctc ctgattgctg
     1381 actattttca tgccattatc ctgaccatcc agtagtaaca gccattcatc cgtggttatc
     1441 tcaacagcat ctgaaggtgc tttatttaaa tcggtaaaaa aaccattttc tttttgtgaa
     1501 tagaagtatc tgtccattta tcaatctcca aaagcaatcc agtaagcaaa aggatttatt
     1561 ccttgctcag tcacagatga catcagggaa aattgcgatg gtgaaacagg caatgctgca
     1621 aaactaacca ttgttgacac acctgaccgt gcattatcgt acgatgcaac aacacaataa
     1681 ttggtattgc tgaaagatat cggcagggtg atatttacag gtgagcctaa tggtcctgat
     1741 gctgatattc ccatttgaat gatggttcca tcaggcaatt ttctccagcg attagaactc
     1801 ggatttcttt tccaggctga catatccggt atctgatttt ctcctgtacc cacatccctt
     1861 ttcg
//
LOCUS       DAHBHC010000267         1818 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHBHC010000267 DAHBHC010000000
VERSION     DAHBHC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.272"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(258..875)
                     /gene="tdk"
                     /locus_tag="M2A35_005402"
     CDS             complement(258..875)
                     /gene="tdk"
                     /locus_tag="M2A35_005402"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCB7201530.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVHELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1480..>1818
                     /gene="hns"
                     /locus_tag="M2A35_005403"
     CDS             1480..>1818
                     /gene="hns"
                     /locus_tag="M2A35_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCB7201531.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQG"
BASE COUNT          551 a          408 c          367 g          492 t
ORIGIN      
        1 tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct tcatcatatt
       61 tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca caagtcaacg
      121 tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg agaagggtat
      181 atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg ctcaccctgt
      241 cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt taatgagccg
      301 acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc attaccacca
      361 attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg cagcaccatg
      421 cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc ggaccatgcc
      481 agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa accataacaa
      541 agtacgggta tatcgagttg atcgacaacc tccgataatt catgtacttg ttgtctggtt
      601 aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg ttccgcacga
      661 atctcatcaa ataatgatga attttggtta aataattttg caggcgatga caaacctata
      721 cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata tacgacagtg
      781 cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga cttacccgca
      841 ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc aatgatatga
      901 aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca gtatgcacag
      961 aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag ctatcattac
     1021 aactgcctcg cttgttataa gcgggtaaat gactgctggt aactattcac aatctttaac
     1081 ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata ctaagtccat
     1141 gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc tcgtaaacac
     1201 aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata aaatgtgaca
     1261 tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata gggctatatg
     1321 ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa aataaagaac
     1381 aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct ctattattac
     1441 ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc acttaaaatt
     1501 ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga aacgctggaa
     1561 gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga aagcgcggct
     1621 gctgctgaag ttgaagagcg cactcgtaaa ctgcagcaat atcgcgaaat gctgatcgct
     1681 gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc tggcaccaaa
     1741 gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg cgaaactaaa
     1801 acctggactg gccaaggc
//
LOCUS       DAHBHC010000268         1755 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHBHC010000268 DAHBHC010000000
VERSION     DAHBHC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.273"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(45..278)
                     /locus_tag="M2A35_005404"
                     /pseudo
     CDS             complement(45..278)
                     /locus_tag="M2A35_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            277..684
                     /locus_tag="M2A35_005405"
     CDS             277..684
                     /locus_tag="M2A35_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCB7201532.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDIS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            681..1235
                     /locus_tag="M2A35_005406"
     CDS             681..1235
                     /locus_tag="M2A35_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201533.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSGRDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRAAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            1222..1611
                     /locus_tag="M2A35_005407"
     CDS             1222..1611
                     /locus_tag="M2A35_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCB7201534.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDSDIVTYQGRDYRVTFVD
                     PYTAYGVGFVQAHCELLPFDGGTPVEQ"
     gene            1601..>1755
                     /locus_tag="M2A35_005408"
     CDS             1601..>1755
                     /locus_tag="M2A35_005408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201535.1"
                     /translation="MSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGLSAGMV
                     RPRWQKE"
BASE COUNT          412 a          442 c          518 g          383 t
ORIGIN      
        1 gtcgagccag tgtacaaaga aaccatttcg tacgttgatg gcatttaaca gccatatggc
       61 cccctggcgg ggccattaag gatgacccga tggcaaaaaa taatgcagta atacacgtac
      121 ataccccgtt tgtgctcacg cttcccgacg gttcacggcg cgagtttgtt aaaggccgtc
      181 atgctgtgga ggaagacgtt gccacgcact ggttcactcg tgcgcacgcg gaagtatccg
      241 ttggcaaagc cacagacgcg cgtaacgagg taaaaaatgc caaagaatca aagtctgcca
      301 gcggtaagtg attttcgccg cgacttcccg cagtttgctg accctgccaa atatcccgaa
      361 gcgcaaatcc agtttcgtct gaatctggca gatgaactgc tgagcgaaaa cgtcaccggc
      421 aaaaagttgt ttccgtactt tgccggattg ttcgttgcgc actacatgac gctctgggcg
      481 gctgacagca gggcgatgct ggctggtggc cctggcggct caaccaatgg tgttcagtcc
      541 tcaaagtccg ttgacaaggt aagtgtcagt tatgacatca gcgcgacgct gaatcctgat
      601 gcaggcttct ggaataacac ccgatatggc gctgaatttt atcagttgat cacgatgttc
      661 ggtgcaggcg gtcgccagct atgagtttca aaagcggtgt aacaacgagg gtggataacg
      721 ctaaggccat tctggatgcg ctcaggtcgt taaccaaaaa agatgtgctg gtcggcatcc
      781 cttcggaaga cagcgggcgg gatgatgttc cgtttggtaa tgcgggcatc ggttacctca
      841 acgaatacgg ctcaccagag cagaacatcc cgccacgacc tcacctggtc cccggcgtta
      901 aatcggcaga agagcagacg gtgccgcagc tcaaagccgc ggcgcaggct gcacttgatg
      961 gtaatgctgc gggagcagaa cgcgcactca accgtgccgg aacgctggcc gctaatggcg
     1021 tcaggcgtta catgaccatt accggcttta cgccgcttgc tgacagcact gttgaagccc
     1081 gggctcgtcg ggggcgcaag ggggcaacac tggaacttgc ccggcgtgct gctggcgaat
     1141 ctccgggaac cgatctggcg aaaccattaa ttgacaccgg gcaatatcgc agagccatta
     1201 cccatgttgt gagggataaa gatgccgact cttgatgtaa cagatgtgct ttttgacccc
     1261 gatttttgcg acttcaattt gtgggtaaca cgccgagtgc aaacggtgga tgaggacggg
     1321 atcggcagcg acagtgaagt taaaaagcag tttgccggag tcgtaactgt tgatcgctct
     1381 ctggaaaacc gccgtatgca ggcagggcag gtaatcagtg gtgcaattct gattgtgacg
     1441 actgagcgac tgacgcaggg acagactggc cgtgattccg atatcgtgac gtatcagggc
     1501 cgtgattacc gtgtgacctt cgttgacccg tatacagctt atggtgtcgg attcgttcag
     1561 gcgcattgtg agttgctgcc gtttgatggg ggaactccgg ttgagcaata acaccagcac
     1621 agagcgcgga tggctgatac caaccagtgg cgatccggat tatgacgaag cgctcgacag
     1681 gctgttaagc cagtggatgc gtaacgtttc cggtctgtct gccgggatgg ttcgtccgcg
     1741 ctggcagaaa gagca
//
LOCUS       DAHBHC010000269         1750 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHBHC010000269 DAHBHC010000000
VERSION     DAHBHC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.274"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            205..501
                     /locus_tag="M2A35_005409"
     CDS             205..501
                     /locus_tag="M2A35_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201536.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLMKQLGQINDELGALLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTSPV"
     gene            607..1428
                     /locus_tag="M2A35_005410"
     CDS             607..1428
                     /locus_tag="M2A35_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCB7201537.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          479 a          358 c          446 g          467 t
ORIGIN      
        1 ccaaaagcag gcaaaaactt ttgcggtctg cccccggaca gtcgtttccg gccgtggcag
       61 tgatgcccct tgcggcgaag gttctcaaac gggtgatggt taataccgga gggaacaaac
      121 ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacgggt gatgggggct
      181 gaaaacatca gatacagagg aattatgagc gcattttctt ttaacaccac ttatcagcca
      241 accggcgatc agcagaagga cgcaatcgca caaatcgaca tcatgcagaa tcgtgctgtt
      301 caggcaaatc ttgcatacca gagcagcctg gatgcggaaa cgctgatgaa acagttaggg
      361 caaatcaatg atgaactggg ggcgctgttg gacagcgtgg aaaatcacac gccagttatc
      421 agaaaaaaag ccagtgattt gagtgcactg atgatgcttt tcagccaaca ggccggtcag
      481 cccatgacca gtccggtgtg atgatttttt ccctgattgt tgtcgatttg ttaataataa
      541 tcagggggag gaaattaagc gaaaaaattt ttgacttatt ttttctttca gcgaggagtg
      601 aatattatgc gactggcttc ccgttttggc cgatataatt ccattcgccg tgagcgcccg
      661 ttaacggatg acgaattaat gcagtttgta ccgtctgttt tttctggtga caaacatgaa
      721 tcccggagtg agcgttatac atatatccca accatcaata tcatcaataa gttacgtgat
      781 gaaggttttc agccgttctt tgcctgtcag agtcgggttc gtgatttggg gcgtcgcgaa
      841 tacagtaaac atatgttacg tcttcgcagg gaagggcata ttaacgggca ggaagttcct
      901 gaaattatcc tgcttaattc acatgatggt tcatccagtt atcagatgat ccccggaatt
      961 tttcgttttg tctgcacaaa tggcctggtg tgcgggaata attttggcga aatccgcgtt
     1021 ccacataaag gtgatattgt cgggcaggtt atcgagggag cgtatgaagt gctcggtgtc
     1081 tttgataagg tcactgataa tatggaggcg atgaaagaaa ttcatcttaa cagtgacgag
     1141 caacatttat ttggcagagc tgcactgatg gtcaggtatg aagatgaaaa taaaacgcca
     1201 gtgacacctg aacagataat tactccccgt cgtcgggaag acaaacagaa cgatctctgg
     1261 acaacctggc agcgggttca ggagaatatg ataaaaggtg gattatcggg gcgaagtgcc
     1321 tccgggaaaa ataccaggac aagagccatt acaggtattg atggtgatat aagaatcaac
     1381 aaggcgttat gggtgattgc cgaacagttc agaaagtgga agtcatgata atataagtaa
     1441 agccccgaaa aaattttcgg ggctttactt atattatcat ttttcggtat tgctgtgtcg
     1501 tctgatatac ctcaatttca tcaaccggct ttccggttgt ctgtaaatat tgtgcccgcg
     1561 aaaaatggtc tggcaggagg ctgcgcagtt accgggtata tataagatgg gttcccgctt
     1621 cgcggggctt cggccagtca gtattgtcac tcatatgata tttttgtgtg tggccttcca
     1681 tgccgctttt gcggcataac cggtatctga caatgtctgt aagattaatt gttctgcacc
     1741 ctgttaattt
//
LOCUS       DAHBHC010000270         1735 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHBHC010000270 DAHBHC010000000
VERSION     DAHBHC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.275"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            86..352
                     /gene="ariR"
                     /locus_tag="M2A35_005411"
     CDS             86..352
                     /gene="ariR"
                     /locus_tag="M2A35_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCB7201538.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            465..713
                     /locus_tag="M2A35_005412"
     CDS             465..713
                     /locus_tag="M2A35_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201539.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            1045..>1735
                     /locus_tag="M2A35_005413"
     CDS             1045..>1735
                     /locus_tag="M2A35_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCB7201540.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILL"
BASE COUNT          517 a          339 c          348 g          531 t
ORIGIN      
        1 gcgacagctg acaataaaag tatgttcaat ggtaagaaaa taaacagaat acattaaaat
       61 ttcataagta agatgagagg ttaccatgct tgaagatact acaattcata atgcaataac
      121 tgataaagcg ttagcaagtt actttcgcag ttcgggtaat ttgttagaag aagagtcagc
      181 agtgttaggg caggctgtca ccaatttaat gctttcaggc gataatgtta ataataaaaa
      241 tattatctta agtctgatac actccctgga aacaacaagt gatattctca aagctgatgt
      301 gattagaaaa acactggaaa tcgtgttgcg atacacagct gatgatatgt aacttctaat
      361 tattaagtat aagtttatag aaaactcatt catcttattt ttgtctgtcg ctttagacta
      421 tacacagcat aattttattg ggttaatatt tctacgaggc tgacatgaat aattcaatcc
      481 cagagagatt tatttttcaa tgtgctttgt ttaaaaatct cgagagggag gtgttcatga
      541 cgcatgggta tgttgatagt catattattg atcaggcttt acgcttgcgt ttaaaagatg
      601 aaaccagtgt gattctttct gatctctatc tgcaaatatt gcagtacatt gaaatgcata
      661 agactacact aacggatatc attattaatg acagggaatc cgtgctctct tagaacttga
      721 gtaaggtttt agaataaaca tcagctgtat caccatgctg atgcaaagtg agcatttcag
      781 gcgttatgct ttcttattat gtccgcaata tcaggtgtca agaatggaga gttctcgctc
      841 tccattcttg acgcctgata tcccgcctaa cttattttgt actgcctaca ttatgctcaa
      901 taattaatgg gttagatcaa gatggagaaa attttatctt cggcgtggat atttcttatg
      961 ctctctacca tgctctctgt agatgccagc gcgagcgaat gaatggcatt ttcatgtatt
     1021 aaaccctttg gccgggacaa gcaaatgcgc aatacactca tacccatcct cgttgcgata
     1081 tgcttgttta ttaccggtgt cgctattttg aacatccagc tctggtattc cgcaaaagca
     1141 gagtacctgg cgggagcgag atatgccgcc aacaatatca atcatatact tgaagaagcg
     1201 tcacaagcga ctcaaacagc ggttaacatt gccgggaagg aatgcaacct cgaggagcaa
     1261 tatcagcttg gcactgaagc agctctgaaa cctcacctgc gcacaatcat cattctcaaa
     1321 cagggaatag tctggtgtac atccctgcct gggaatcggg tcctgttgtc tcgtattcct
     1381 gttttcccgg acagtaattt actgttggct ccagcaatcg acaccgttaa tagattacct
     1441 atcctgctct atcagaacca atttgcagat acgcgcattt tggttacgat aagtgatcaa
     1501 catattcgcg gggcgcttaa tgtacccttg aaaggggtaa ggtatgtatt acgcgtggcg
     1561 gatgacatta ttggaccaac gggtgatgtg atgacgctta atggacatta tccctatacc
     1621 gagaaggttc actccacaaa atatcatttc actattatct ttaacccgcc gccactcttt
     1681 agcttctaca gacttatcga taaaggcttt ggaattttaa tatttattct gttaa
//
LOCUS       DAHBHC010000271         1734 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHBHC010000271 DAHBHC010000000
VERSION     DAHBHC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.276"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(80..343)
                     /locus_tag="M2A35_005414"
     CDS             complement(80..343)
                     /locus_tag="M2A35_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201541.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            complement(425..1114)
                     /locus_tag="M2A35_005415"
     CDS             complement(425..1114)
                     /locus_tag="M2A35_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCB7201542.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            1219..1449
                     /locus_tag="M2A35_005416"
     CDS             1219..1449
                     /locus_tag="M2A35_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201543.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            1519..>1734
                     /locus_tag="M2A35_005417"
     CDS             1519..>1734
                     /locus_tag="M2A35_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCB7201544.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSR"
BASE COUNT          472 a          410 c          349 g          503 t
ORIGIN      
        1 aaaatgattc tgccgccttt gcaattttat catttaggtt tggagcttcg taataatcgt
       61 gtttcataat taattacgat tagtttgttt tagctttgtc atgctggtcg cgaatgaagt
      121 cagccaattt aagcatcaat tcttcgggca ttctaatggt tgcaatgttg tactttatgt
      181 tctcaacatc gtcactttca tcaacaggcc attcagttac attatgaaaa aaagaaattg
      241 aacctaggcg ctgagagccg tcaccaaacg aagagtagcc aaacgcgtcg gcgtatgcat
      301 cgacacagtt atttgatttt ttaacaagtg ttttattact catttttgcc tttcccgtaa
      361 tgttattttt tataaggtat tttcttcagc gtattaccca aaagcctcat caacacgcgg
      421 ctgattatcc gtgttttctg tacgtctgcg gcatgctccc aataacctta ccgaatatga
      481 atacccggtt catctcgtct ttctcgatag ggtcccacgg tgagtagctc ttgttatcag
      541 agataaccag cagtttatcc ttcatcattt gcaggcgctt tacatgggcg gtgtcgtcgt
      601 acagaaatgc atagatacca tcaccgtcga aagatttaac cgtgatatca acgaacagca
      661 gatcacctgg ttcgatcgtt cctgacatgc tgtcaccacg cacgttaatg atgcggatat
      721 tttctgcctt cctaccatcg aacatgtgac gagcatcgtc aaacgagtac tcaaccgagc
      781 gtagaacttc tacaaactca cggttgatga ctcccggccc agcactgact tctatatcaa
      841 gaacgtcaat cttgaagtat ttggaatggc tgacagttga ttgtattggt tgcactgtac
      901 tgtctgacat atttccaacg ccagaagata accattctgc gcgcacaccc aaagcgttcg
      961 cgatctccac gattttagtt gtttgattag ctttccctgt ttcgattttc tgaatagcag
     1021 cctggctaac cccgaccaaa tccccaagcg ccttttgtgt aaggcctcgc gctaatctgg
     1081 cttctttaag tctttctgag agtgttgttt tcatagtcca aatgtacaac caaggtttta
     1141 ttccatcaaa cgaaaatggt tgttgactaa aaacaaccat ggttttaatc ttgattcaaa
     1201 ttaaccacgg aggttgttat gaacccagct atcaaaacag cgatcaatat cgttggttca
     1261 caaaagaaac tgggcgctgc ctgcgaagtt tcacagcagg ccgtctataa gtggcttcac
     1321 aacaaagcaa aggtatcccc tgaacatgtc ggcagcattg ttacagctac tggtggagta
     1381 gtgaaggcat accagattcg cccggatctt ccgaagttgt ttccacacac cgaaaagaac
     1441 gcagcttaaa tttccatttc acgctcttta acaataagca atcaacttaa cagtcaattc
     1501 aaactaaagg agtcaattat gcaaccactt ccataccaac agactagcgg atttagcccg
     1561 actgcggtga taaatcgttc tcaaacaaaa caggtgccag gccacgaaaa aatccgtgat
     1621 gccgtccgcg cctggtcggc tgtagataat caggatgtcg ttgccacact cattgtgaat
     1681 gagtatcggg agcagggcgg cggcaccatc gatttccctg atgatgtcag ccgt
//
LOCUS       DAHBHC010000272         1705 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHBHC010000272 DAHBHC010000000
VERSION     DAHBHC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.277"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(68..310)
                     /locus_tag="M2A35_005418"
     CDS             complement(68..310)
                     /locus_tag="M2A35_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201545.1"
                     /translation="MKVKTLVSSGLTTAGIVMSSSASAAVTVPSFITADSVGEIGTAI
                     TSVIGIAGTAAFAVLGVSLAARLGIGIIKGFLSRAT"
     gene            complement(336..506)
                     /locus_tag="M2A35_005419"
     CDS             complement(336..506)
                     /locus_tag="M2A35_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201546.1"
                     /translation="MTEFDFSGVLSAINSGVQDGELYWAVLYVWIVCWLLGFGLGVIF
                     TLVCRMFSDVVN"
     gene            complement(644..1069)
                     /locus_tag="M2A35_005420"
     CDS             complement(644..1069)
                     /locus_tag="M2A35_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201547.1"
                     /translation="MNEFYDSFPFPVPEGFATGMTMAGYFIHATVINDEELKPGGTHY
                     NPEAKPKYAIVIAYPYEDKKLKVRREEHERFSCTEEDFKFFNSCPDLKGKPVYLTVDV
                     NSWATGSERHGVWYRFISGSMKRFDGQPLGALPAGKEKG"
     gene            complement(1100..>1705)
                     /locus_tag="M2A35_005421"
     CDS             complement(1100..>1705)
                     /locus_tag="M2A35_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201548.1"
                     /translation="GITALNDRYSARSFCDAVSRAACWQKATRLRLFQFFGVPSVTAY
                     RQMRSFRAPLEAHHINMQQFTPQQVAELEAIRMACDAGDFRTYILLNGGFFCSERLLR
                     PFYVQPQDGGKPRINRYGEPCAPVISGFMFGPVPVITRFMGCVVRRMTPAEKIRAEEI
                     RSGSSDESVFISSASRHRTTRSRAAGGGGGADPWTCDNNCP"
BASE COUNT          515 a          436 c          405 g          349 t
ORIGIN      
        1 tatgccaaga ctcaccccct gaataagcaa aaaaagcgcc ccgcagggcg ctgttgttaa
       61 ataactatca ggttgcgcgg gaaaggaagc ctttaatgat accgataccc agacgggcag
      121 cgagtgaaac accaagaacg gcaaatgcag ccgtacctgc aatgccaata acactggtaa
      181 tggctgtacc gatttcacca accgaatcag cggtaataaa agaaggaaca gtaaccgcag
      241 cagaagctga agacgacatt acaatacctg ccgtagtcag tcccgatgat accagggttt
      301 ttactttcat aaaattcctc tttgacgatt aaaaattaat taacaacatc gctgaacatc
      361 cggcagacca gagtgaaaat aacaccaagg ccaaaaccaa gaagccagca gacaatccag
      421 acatacagaa cggcccagta aagttccccg tcctgtacgc cggaatttat tgcggataaa
      481 acaccgctga aatcaaattc agtcataata acgctgaaca gtaatgcttt gtgagtcaca
      541 ggcgcgaatc agttttattc cgatgccaaa acctgaggaa aataaaatca gtgcaggcgc
      601 agcgaataaa acaaagttca gaaaatcagc gtcagacata tcatcagcct ttttctttac
      661 ctgcgggcag tgcccccagt ggctgaccgt caaaacgttt catggagccg gaaataaaac
      721 gataccagac gccatgacgt tcagagcctg ttgcccatga attcacatcc accgtgagat
      781 aaacaggttt acccttcagg tcaggacatg aattaaaaaa tttaaaatcc tcctcagtac
      841 aggaaaagcg ctcatgctcc tcacgacgga cttttaattt tttgtcctca tacggatagg
      901 caataacaat ggcatatttg ggtttagcct cagggttata atgagtacca ccgggcttta
      961 actcctcatc atttataaca gtggcgtgaa taaaatatcc cgccattgtc atacctgtcg
     1021 cgaaaccttc aggtacagga aacggaaaac tgtcgtaaaa ctcattcata aatcagactc
     1081 ctcaatgtta aaaattcaat cagggacagt tattgtcaca agtccaaggg tcggctccgc
     1141 cgcccccgcc cgccgcgcgg gagcgcgtcg tgcggtgtcg tgaagccgac gaaataaaca
     1201 cagattcatc gctgctgccg cttctgattt cctctgcgcg gattttctct gcgggagtca
     1261 tacggcggac aacgcacccc atgaaacggg ttatgaccgg aacggggcca aacatgaaac
     1321 cggaaatcac cggcgcgcag ggttcaccgt aacggtttat acggggctta ccaccgtcct
     1381 gcggctgaac gtaaaacgga cgaagcagac gttcagaaca gaagaaaccg ccgttcagca
     1441 ggatataggt ccggaaatcg cctgcatcac aggccatgcg gatggcctcc agttcggcga
     1501 cctgctgcgg cgtgaactgc tgcatattga tatgatgtgc ctcaagcggt gcgcggaagc
     1561 tgcgcatctg gcggtaagcc gtgacggacg gaacgccaaa gaactggaaa aggcgaaggc
     1621 gcgttgcttt ctgccagcag gcagcacggc tgactgcatc acagaatgag cgggcagaat
     1681 aacggtcatt cagggcggta atacc
//
LOCUS       DAHBHC010000273         1705 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHBHC010000273 DAHBHC010000000
VERSION     DAHBHC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.278"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..319
                     /locus_tag="M2A35_005422"
     CDS             <1..319
                     /locus_tag="M2A35_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HCB7201549.1"
                     /translation="MVYEYCRKRGLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDA
                     ELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKG
                     ASV"
     gene            316..606
                     /locus_tag="M2A35_005423"
     CDS             316..606
                     /locus_tag="M2A35_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HCB7201550.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            680..1120
                     /locus_tag="M2A35_005424"
     CDS             680..1120
                     /locus_tag="M2A35_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCB7201551.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            1117..1644
                     /locus_tag="M2A35_005425"
     CDS             1117..1644
                     /locus_tag="M2A35_005425"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCB7201552.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAT"
     gene            1641..>1705
                     /locus_tag="M2A35_005426"
     CDS             1641..>1705
                     /locus_tag="M2A35_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCB7201553.1"
                     /translation="MRRQRRSFTDIICENCKYLPT"
BASE COUNT          469 a          379 c          486 g          371 t
ORIGIN      
        1 tatggtttac gagtattgcc ggaagcgagg cctgtatccg gatgcggagt cttatccgtg
       61 gaaatcaaac gcgcactact ggctggttac caacctgtat cagaacatgc gggccaatgc
      121 gcttactgat gcggaattac ggcgcaaggc tgccgatgaa ctgtcctgta tgaccgcacg
      181 aattaaccgt ggtgaggcta tacctgaacc agtaaaacaa cttcctgtca tgggcggtag
      241 acctctaaat cgtgcacagg ctctggcgaa gatcgcagaa atcaaagcta agtttgggct
      301 gaaaggagca agtgtatgac gggcaaagag gcaattattc attacctggg gacgcataat
      361 agcttctgtg cgccggacgt tgccgcgtta acaggcgcaa cagtaaccag cataaatcag
      421 gccgcggcta aaatggcacg ggcaggtctt ctggttatcg aaggtaaggt ctggcgaacg
      481 gtgtattacc ggttcgctac tagggaagaa cgggaaggaa agatgagcac gaacctgatt
      541 tttaaggagt gtcgccagag tgccgcgatg aaacgggtat tggcggtata tggagttaaa
      601 agatgaccat ctacatcact gagctaataa caggcctgct ggtaatcgca ggccttttta
      661 tttgggggag agggaagtca tgaaaaaact aacctttgaa attcgatctc cagcacatca
      721 gcaaaacgct attcacgcag tacagcaaat ccttccagac ccaaccaaac caatcgtagt
      781 aaccattcag gaacgcaacc gcagcttaga ccaaaacagg aagctatggg cctgcttagg
      841 tgacgtctct cgtcaggttg aatggcatgg tcgctggctg gatgcagaaa gctggaagtg
      901 tgtgtttacc gcagcattaa agcagcagga tgttgttcct aaccttgccg ggaatggctt
      961 tgtggtaata ggccagccaa ccagcaggat gcgtgtaagc gaatttgcgg agctattaga
     1021 gcttatacag gcattcggta cagagcgtgg cgttaagtgg tcagacgaag cgcgactggc
     1081 tctcgaatgg aaagcgcgat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca
     1141 gcacacgaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
     1201 tttggattct ggctggattc ggcagcgagc gacaaaaacg ctctgtgcgc tcactggcta
     1261 actgaggctg acgacgcgct aaattctgag tggataagcc acggtgcaat ctggaataac
     1321 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
     1381 cgacagacgg tagtgatgct tgtgccagag gatatgtctg tcggatggtt cagcaaggct
     1441 ctggagagtg ttgacgaagt tcgtattatc actgatggac ggattaattt tatcgaacca
     1501 tcgacagggc tggagaagaa gggaaacagc aaaggctcca tgctgctgat ttggcgaccg
     1561 ttcattagtc ctcgacggat gtttactatc gtatccaaag cggcattgat ggcgatcggg
     1621 cagggcgtca ggagggcgac atgaggcgac aacgacgaag tttcaccgac atcatctgcg
     1681 aaaactgcaa ataccttcca acgaa
//
LOCUS       DAHBHC010000274         1695 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHBHC010000274 DAHBHC010000000
VERSION     DAHBHC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.279"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            13..822
                     /locus_tag="M2A35_005427"
     CDS             13..822
                     /locus_tag="M2A35_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7201554.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDTNIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            815..1297
                     /locus_tag="M2A35_005428"
     CDS             815..1297
                     /locus_tag="M2A35_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201555.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMFNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLVQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1330..1410)
                     /locus_tag="M2A35_005429"
     CDS             complement(1330..1410)
                     /locus_tag="M2A35_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201556.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1446..>1695)
                     /locus_tag="M2A35_005430"
     CDS             complement(1446..>1695)
                     /locus_tag="M2A35_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201557.1"
                     /translation="KVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLILSSIDSLS
                     KLVINNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          596 a          298 c          300 g          501 t
ORIGIN      
        1 gtttattgac gtatgtactg gattattaac gataatatcg agttctggcc tgagcaccga
       61 aaattaatat cggtacataa cgccgatctt aacgtcgttc tgacaacgcc agccagtcga
      121 tgtttatcac ttctacttga agcttttcct gatgtggttg cacaacaaga ttttttcacc
      181 agagtctggg aagaagaagg tatgcgtgtg cctactaaca cgttatatca gaacatatcc
      241 attatcagac gcggatttcg cgctgttggt gatactaccc actcgctaat tgcaaccgtg
      301 ccgagaagag gattcaagat ccataatgac actaacatac aaaatcatgt aataaactcg
      361 tcaacagacg cacatacaca caatgcccca cctgccataa aagttaatgc gggatacaaa
      421 gagagcattg gtggcgcaaa gaatttcaat aacaaaatcc tcaaacatat aaaaagtcat
      481 ctaattatgt tgagcgcatt tgtcataggc gcatattctg catattggct atggaataat
      541 aatcaaccga agccattttt caaggattac aagactgttg cggagattaa tggctgccat
      601 tttaatgtaa cagaagatac aattgacggg ttgaaagagt tcgataaata taaaacacgg
      661 atactggatt cggggattaa ctgtaaaaaa catccgtggt tatacttccc tcttgcaaaa
      721 tcctcaccgg ggatgattgt tatggcatgc aataaaaatt ataaccaaca tgaagtggca
      781 gactgtttaa ccctgtctta ccgtgaggtt aatcgtgatt gattataaaa aaaacttatt
      841 atttatttta gtttttatca gtgggtttat tctcttcact gtctattctt acactgcaga
      901 aaaaatgata tataacgaaa catgcactgc aaattgggta atttttaatg accagggacg
      961 agcaaatctt accatcgact ttatgtttaa caaaaaaaat aaaacaggaa ctgttgcgtt
     1021 gagtggaacc tggcaacaag gtaacagaga aagtaaatca atacggcgaa atattgaata
     1081 cacatggatt gaaaactatg acacagccca tttaacatca aagaaagtta ataagtttga
     1141 gattatggat caagttgatg atgatagact tgtacaactt attcctgatt tttatgtctt
     1201 tccagaaaaa agtgtaagct ataatatact aaagcaaggt aagcatgctt ttattttgag
     1261 cattggtaac agagcaataa tgcattgtgc aaggtaaact aaaatagata acaaaaagat
     1321 ggaacctcgt taatcataaa acaataaaat taaatattct cgcagtatat ggcagtctaa
     1381 agcatcaaag ttttgatcaa catctttcat tttagacatc tccttgcaat gtaaaatatc
     1441 atgtatcaac aacatctggt gcaaatccat gagtcgaact cgacattcat cttagctcga
     1501 ttattattcc ttcgtacagt cgatgtaaac aatacagaaa gaggattatt aataaccagt
     1561 ttactcagtg aatcaataga ggaaaggatt aacgtttctt taaaagaatt aatttcatca
     1621 tctgttaaac taaactcatc attgacagat cgtgagatat aactgttttt aactttactc
     1681 tttacgttga cttta
//
LOCUS       DAHBHC010000275         1690 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHBHC010000275 DAHBHC010000000
VERSION     DAHBHC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.280"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>1690)
                     /locus_tag="M2A35_005431"
     CDS             complement(<1..>1690)
                     /locus_tag="M2A35_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7201558.1"
                     /translation="NSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQ
                     AALPGVLSMATAGGMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRA
                     LGETMKYAGPVAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAA
                     EALKELGVSVSDAGGKMRPMEDLLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMAL
                     VDAAGDGSLPKLRKELEGARGEAERTAKVMADNLDGDLKNLDSAWEGLRIRISDLVDG
                     SLRSVTQWLTRVVSKVTALAQAHPVLTRQLLIAGGALLAVTATVGSLSLALGVLAGPL
                     AKLRLGFSLLTGSMNAVRLLPALWGMVTGSVSLLGGAIGALFSPVGLIAAAFVAAAVL
                     IWKYWEPIKAFYAGVFSGIMERLAPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPM
                     ESSKETLDKCTSAGEVFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARK
                     KIEDAQRAAILQDKVALFQGDIAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGT"
BASE COUNT          370 a          530 c          427 g          363 t
ORIGIN      
        1 gtaccggtat tgctgtctgt gagcggtttg ttgtctttgg ggttatcacc tccggtgcca
       61 ttgccatttt ccacaggctt cggcggattg attttcgcaa tgtctccctg aaacagagca
      121 accttgtcct gaagaatggc cgcacgctgc gcgtcttcga ttttcttgcg cgccctttcc
      181 gcttcatccg gaagaacacc gagtttttca agtatccacg ccaacgtatc cagcagcatt
      241 tttgcaggcg tcaggacaag ctgtaacgca ccgccaagaa cgttaccgaa tacctcgcca
      301 gcacttgtgc atttatccag cgtttccttg ctggactcca tcggtgacaa cagcgattta
      361 aaccagttaa acacctggct gatcccgctt ccgattgcgt caaaaacagg accaaaccgt
      421 tcaaaggttt cgcgtaacgg ggccagccgc tccataatcc cgctgaacac cccggcataa
      481 aacgccttga tgggttccca gtatttccag atgagaactg ccgcagccac aaacgcagca
      541 gcaatcaatc cgaccgggct gaacagcgcc ccgatagcgc cccccagtaa cgaaacggaa
      601 cccgtcacca ttccccatag tgcaggcagg agcctgacag cattcattga tccggtcagg
      661 agggaaaaac caagacgcag ttttgccagt gggccagcaa gcacaccaag agcaagcgac
      721 aacgagccta cggttgcagt cactgccagc agcgcaccgc ctgcaatcag tagctggcgc
      781 gtcagtacag gatgggcctg cgccagtgcc gtcacttttg agaccacccg cgttagccac
      841 tgcgtgacag aacgcagcga accgtcaacc agatcactga tgcgaatacg aagaccttcc
      901 catgcgctgt cgagattttt caggtcgcca tcaaggttgt cggccataac ctttgccgtg
      961 cgttcagcct caccgcgtgc gccctcaagt tcttttctca gtttgggtaa ggaaccgtca
     1021 cccgccgcat caacgagtgc cataaacgat gtgaaagcct cttccccggc gatgtcctta
     1081 aagaaggata cccggtcaac ttccccgtat ttgcgggtag ctttataaag gtcagccagc
     1141 agatcttcca tcgggcgcat tttgcccccg gcatccgaga cggacacgcc cagctctttc
     1201 agcgcttctg ccgctgcctt tggcggtgat gccagacgag ccaggctggc acgcattgcc
     1261 gtcccggcat cactccctct gatgcccata ttcgccagca cgccagccat cgctgcggcc
     1321 tgctccagcg atattcccag cttacccgcc accggacctg catatttcat ggtttcgccc
     1381 agtgcgcgaa ggtcagtgtt ggtacgggta aacgctgcgg tgagtgtgtc accgacccgg
     1441 tccatctggt cagcagaaag gccgaactgc gtcaggatat ttgagccaat atctgccgtc
     1501 tcgccgagat ccataccgcc agccgttgcc atgctcagta cgccgggaag cgcagcctga
     1561 atggcctgcg gagtgaagcc agccattgca agaaatgcct gtccactggc ggcatcgcct
     1621 gcggtgaact gcgtttcaga gccaagtttt aacgcctgct cacgcagcgc cttaaactgc
     1681 gggctgtttt
//
LOCUS       DAHBHC010000276         1684 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHBHC010000276 DAHBHC010000000
VERSION     DAHBHC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.281"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..593)
                     /gene="cdtB"
                     /locus_tag="M2A35_005432"
     CDS             complement(<1..593)
                     /gene="cdtB"
                     /locus_tag="M2A35_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I nuclease
                     subunit CdtB"
                     /protein_id="HCB7201559.1"
                     /translation="MKKLLFLLMILPGISFADLSDFKVATWNLQGSNAPTENKWNTHV
                     RQLVTGSGAVDILMVQEAGAVPASATLTEREFSTPGIPMNEYIWNTGTNSRPQELFIY
                     FSRVDAFANRVNLAIVSNRRADEVIVLPPPTVVSRPIIGIRIGNDVFFSTHALANRGV
                     DSGAIVNSVFEFFNRQTDPIRQAANWMIAGDFNRSPAT"
     gene            complement(590..1303)
                     /gene="cdtA"
                     /locus_tag="M2A35_005433"
     CDS             complement(590..1303)
                     /gene="cdtA"
                     /locus_tag="M2A35_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytolethal distending toxin type I subunit CdtA"
                     /protein_id="HCB7201560.1"
                     /translation="MDKKLIAFLCTLIITGCSNGIGDSPSPPGKNVELVGIPGQGIAV
                     TSNGATPTLGANNTEFPEVSIMSTGGALLTIWARPVRNWLWGYTPFDSVNFGENRNWK
                     VVDGKDAGTVKFVNVAQGTCMEAFKNGVIHNTCDDNSLSQEFQLLPSTNGNVLIRSSA
                     LQTCIRADYLSRTILSPFAFTITLEKCPGAKEETQEMLWAISPPVRAAKPNLIKPELR
                     PFRPLPIPPHDKPDGMEGV"
BASE COUNT          506 a          400 c          305 g          473 t
ORIGIN      
        1 gtagccggtg aacggttaaa atcgcctgca atcatccagt tagcggcctg tcttatagga
       61 tccgtttgtc tgttgaagaa ctcaaaaaca ctatttacaa ttgctcctga atccacgcct
      121 cgattcgcca atgcatgggt tgagaagaaa acatcattac caattctaat gccgatgatc
      181 ggtcgtgata caacagttgg aggtggtaat acaatcacct catcagctct tctgtttgaa
      241 acaatagcaa gatttactct gttagcgaat gcatcaacac gtgagaaata tataaacaac
      301 tcctgtggac gactattggt tccggtattc cagatatact cattcatcgg aataccggga
      361 gtgctaaatt ctcgctcagt caacgttgca gaagctggta ctgcccctgc ctcctgaacc
      421 atcaggatat caacagcacc acttcccgta acaagttgtc ggacatgtgt gttccattta
      481 ttttctgtcg gtgcatttga accctgcaaa ttccaggttg caactttaaa atcgcttaaa
      541 tctgcaaaag aaatacccgg caaaatcatt aacaggaata ataatttttt catactccct
      601 ccattccatc aggtttgtca tgaggtggaa ttggcaatgg tctgaatggt cttaactctg
      661 gcttaatcag atttggtttt gccgctctga ctggtggact tattgcccat agcatttctt
      721 gcgtttcttc ttttgcacca gggcatttct caagggtgat tgtaaaagca aacggtgaca
      781 gtatagttct gcttaaatag tctgctctta tacacgtctg caaggcacta cttcttataa
      841 gcacattacc attagtagaa ggtagtaact gaaactcctg agataacgag ttatcatcac
      901 aggtattatg tatcaccccg tttttaaagg cctccatgca agtcccctgg gcaacattaa
      961 caaatttcac tgtgccggca tctttaccat ccacaacctt ccagttccga ttctcaccaa
     1021 aatttactga atcaaaagga gtataccccc aaagccagtt acgaacaggt ctggcccaaa
     1081 tagtaagcag cgccccacca gtgctcatta ttgaaacttc aggaaactca gtgttgttgg
     1141 ctccaagtgt tggagttgca ccgtttgaag tcacagcaat accttgtcca gggattccaa
     1201 ccaattctac attttttccc ggaggggacg gtgaatcccc gatcccattc gagcaaccag
     1261 taattataag tgtgcacaaa aatgcaatta gttttttatc cactgtattt cctcctctgc
     1321 accacctctt ctttataaaa aaatagaaac aacaaagcaa cgagaatatt actaacaaga
     1381 ttaatgagaa aatgtgaaac cagaacaaaa aacaagttta ttataacacc tgttattatt
     1441 catcaggttg cgaacagcat actcaaatat aattctttat attccccaaa aactatgcaa
     1501 tatgaccata acatctacgg ttcagtagat actcacctgt ctggaaataa gggggcaaga
     1561 acactccaga cttaaatagc cataacaaat atcccccgga cattgcaaca caaaaaccgg
     1621 agccggactc cggttttgtg aagctgtctg gttacttcat cccgccaata ttttcccacg
     1681 tccc
//
LOCUS       DAHBHC010000277         1678 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHBHC010000277 DAHBHC010000000
VERSION     DAHBHC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.282"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..143
                     /locus_tag="M2A35_005434"
     CDS             <1..143
                     /locus_tag="M2A35_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201561.1"
                     /translation="GLDCDVLRIPDEPPRRWFDRGFLREVVREARTEIRSMADALAKI
                     RK"
     gene            684..986
                     /locus_tag="M2A35_005435"
     CDS             684..986
                     /locus_tag="M2A35_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCB7201562.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            1034..1456
                     /locus_tag="M2A35_005436"
     CDS             1034..1456
                     /locus_tag="M2A35_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCB7201563.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSIVHDVVRGLMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMNVVLQALDGEHMS"
     gene            1453..1644
                     /locus_tag="M2A35_005437"
     CDS             1453..1644
                     /locus_tag="M2A35_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201564.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQHAVSLGGQYILQGSRNR"
BASE COUNT          342 a          435 c          534 g          367 t
ORIGIN      
        1 gtgggctgga ctgcgatgtg ctgcgtatac cggatgaacc gccccgccgc tggtttgacc
       61 gtggttttct gcgggaggtg gtccgggaag cccggaccga aatccgcagt atggcggatg
      121 ccctggcaaa aatccggaaa tgatgacact ggcggggtaa atccccgcct ttttcctgcc
      181 ggtcccggga cagagcacaa aggaagtcgc cgccggctcc gctttacccg gccatgaggg
      241 gcatggcatt gtgggttttc agctctgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc
      301 ctccatcgta gccgggcttc tgcgggcggg atgcaaaggt acgcttcgcc gctgcggtca
      361 cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt tcacccgctc agatttcgcg
      421 gtctcaccct ccaactcccg tcagccgtgc gcgtcgggct gcggctttcc ttgcgccctt
      481 gcatccctgc aggcccggca aagatggagg cacggcaccg cccggtgtcg cggaatatgt
      541 gaacaacgga ggaaaacatc atgcaatatg cgaaacctgt cactctgaat gttgaagagt
      601 gcgaccgtct ctcctttctg ccttacctgt ttggccagga ttttctgtat gtcgaggcgt
      661 ctgtgtacgc gctggcgaaa aaaatgatgc cggaatacga aggcggattc tggcacttta
      721 tccgcctgcc ggacggtggc ggctacatga tgccggacgg tgaccgtttt catctggtta
      781 acggtgaaaa ctggtttgac cgcacggtca gtgccgatgc tgcaggcatc attctgactt
      841 cccttgtgat taatcgccag ttgtggctgt accacgacag cggcgatgca gggctgacac
      901 atctgtaccg gatgcgtgat gcacagttgt ggagccacat cgaatttcac cccgaatgta
      961 acgcgattta cgcggcgctg gactgattaa acggacgggg cggcaatgcc gcccctgtaa
     1021 aggagacgag aacatgtact gtactgttaa agaaattatc cgcgaagtgc tggatacaga
     1081 cgtgccggac agtgaatgcg tttttgccgt ggtgctgacc cgtggggatg tgcgtcacat
     1141 agcccaggac tggagtctga cagacgatga gctggaaacc gtcatgcagc ggctggacga
     1201 tgcctttgaa tatggtgcgg atgtcagcat tgttcacgac gttgttcgtg gactgatgga
     1261 ggaaaagcgc gccagccgtc aggtgacagt tccggcggtg atgctggaaa aagtgatggc
     1321 gctggcaggc agtgaaatga agcgcctgta tgccgtcggg agtgagaacg ggggcgacgg
     1381 tgatgcgttc gtcagggagg aacgcgaagc aatgaacgtt gtgttacagg cgctggacgg
     1441 ggagcacatg tcatgaatat cagcacggaa acccgcgaaa ttctgcgtaa ttacaaagcc
     1501 gtgattaatg cgcggcgtcg tgaaatgggg cagaaaccgc tcaccactgc gcagattgtt
     1561 gatgaaatct gcgattttgt ggcgaatcag catgctgttt ccctgggcgg tcagtacatc
     1621 cttcagggca gcagaaacag gtgatacagg ggcaggcggt ggaggccgcc tgtcagtc
//
LOCUS       DAHBHC010000278         1640 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHBHC010000278 DAHBHC010000000
VERSION     DAHBHC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.283"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(84..383)
                     /locus_tag="M2A35_005438"
     CDS             complement(84..383)
                     /locus_tag="M2A35_005438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201565.1"
                     /translation="MPQASFLSLSPIFVVVDYLKERFHWPGVTREETALSLHALSDKA
                     VNNELIPRDNAGFPVFRYSGIPGDGICSENKNVAIWKQDSRKIIMMFFSANNEDI"
     gene            777..1400
                     /locus_tag="M2A35_005439"
     CDS             777..1400
                     /locus_tag="M2A35_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCB7201566.1"
                     /translation="MARNQEKKTPVIVVKKRRVISPTPVADKTETVIPSVPEEPVPVA
                     DTGRNLATSIPDGAQAPAASGKKKKGRPPRRPLRAHWTREFTDQCMKKVKVLFPHLRA
                     EDGGFLPLKVGISRDIPAWLAEHPEAGLTRDEWDCAASCITSRRVYLLRTSVTGVTRY
                     DLDGKPAGQVSEDEAQNARRWLAIRERRWQERQATLTTDGSAEPVAE"
BASE COUNT          410 a          390 c          428 g          412 t
ORIGIN      
        1 aatataaccg atgtgataaa taaaatatta tcatttcagt aatatttatg ctggttgcgt
       61 ggttttatct ggtgatataa aactcagatg tcctcattgt ttgctgaaaa aaacatcata
      121 atgatttttc tgctgtcctg tttccatatt gccacatttt tattctcaga gcagatacca
      181 tctcccggaa taccggaata ccggaatacc ggaaagccgg cgttatcacg cgggataagc
      241 tcgttattca ccgccttatc tgaaagggca tgcagggaca gggcggtttc ctctctggtg
      301 acgcccggcc agtgaaatct ctctttcagg taatcaacaa caacgaaaat gggcgataaa
      361 gataaaaaac tggcctgcgg caaagcaggg ggatatcggc tctggcacac gaactggtaa
      421 aggtatcgtg ttgtcccact attcagggga cgtcattcac ggtcatgtga atatcgctga
      481 gaatgtgatt gttcggcaga acacaaccac gggcgaaaag gtctctgaca acagggaaaa
      541 taatattgtt actggtgata atgtctgtat ccgtgcccat atgtgtatta tctgacttaa
      601 tgtaaaaccg gcggtaatgt aaaaaccgga gccctgtctt ttgttatgga ggatgttacg
      661 cataactgta cttatgttac caggacagaa tcacggataa tcagacgtga tgtatgatga
      721 tattattcgc tgtataatga tattgtttac cggaatttaa tcttgtcggg agatttatgg
      781 ccagaaacca ggaaaagaaa acgcccgtga ttgtggtgaa aaagcggcgc gttatttcac
      841 caacacctgt cgccgataaa acagagacgg ttattccgtc tgtaccggaa gagcctgttc
      901 ctgttgctga tacggggagg aatctggcca catccatccc ggatggggct caggcaccgg
      961 ctgcctccgg aaagaaaaag aaaggaagac ctccacgcag gcccctcaga gcacactgga
     1021 cccgtgagtt cactgaccag tgtatgaaaa aggtcaaagt gcttttcccg catcttcgcg
     1081 ctgaagatgg tggttttctg ccgcttaagg tcggtatctc ccgggatatt cctgcctggc
     1141 tggctgagca ccctgaagcc ggactcaccc gggatgaatg ggactgtgcc gcatcctgta
     1201 tcacctcccg ccgtgtctat ctgctgcgta cctctgtcac cggtgtcacc cgctatgacc
     1261 tcgacggtaa gcctgccgga caggtttctg aggatgaggc acaaaatgcc cggcgctggc
     1321 tggccatccg ggagcgccgc tggcaggaaa gacaggccac actgacaacg gacggtagcg
     1381 ctgaaccggt ggcggaatga tttctcaccc ggacatggtg tcctgttata taccgacagc
     1441 gttgtaccgt gcacatgacc gtttacttcg tatgtccggg cgctcctgag acgggtttta
     1501 cagtctcagg gatgactgcc tggcgctcac ggatgtcact ttctctgtct tcgctgcctg
     1561 agtggcccgg tggcaggata ttgcctgcct gtggcgaaag cagcatgata ccggtaccgt
     1621 gttttacagg agtaacggta
//
LOCUS       DAHBHC010000279         1638 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHBHC010000279 DAHBHC010000000
VERSION     DAHBHC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.284"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>1638
                     /locus_tag="M2A35_005440"
     CDS             <1..>1638
                     /locus_tag="M2A35_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCB7201567.1"
                     /translation="NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERK
                     ETGKYTSVVDLARARVNQQHTENSTGKISLVIAAIHREYKQTWKTLDDELAYALWPGD
                     VDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDI
                     HKDPIALNKYICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAP
                     EAEPSVEREGPFYFLSADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYT
                     GLPQNVDTAEDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPN
                     DPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCT
                     ACGQTGGGNGPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQH
                     HDSDNETGETADHSIKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDP
                     QTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA"
BASE COUNT          524 a          390 c          416 g          308 t
ORIGIN      
        1 ttaacgccat gtatccggag cttaagctgc atgccatcgg gtgggttaag cataaatgta
       61 agcctggtgc caaatggccc gaaattcagg cagagatgcg catctggaaa aaacgtcgcg
      121 aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt tgatctcgcc cgcgccagag
      181 tcaatcaaca gcacactgaa aattcaacag gaaaaatcag cctggtcatt gctgccattc
      241 atcgcgaata caagcagaca tggaaaacac tggatgacga actggcctac gctctctggc
      301 ctggtgatgt ggatgccgga aacattgacg gcagcatcca tcgctgggca aaaaatgaag
      361 ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc atcaatgcgc aaacagcctg
      421 atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgagcgt ccgatcgaca
      481 ttcacaaaga tcccatagca ctgaacaaat atatctgcga atacctgacg acaaagggcg
      541 tgtttgagaa tgaagaaaca gacctgggca ctgttgatgt tctccagtca tcagaaacac
      601 aaactgatgc agtggaaact gaggtatctg atatcccaaa aaatgaaacc gcgccggaag
      661 ctgaaccatc tgtagagcgt gaggggccgt tctatttcct ctccgcagat aaggacggag
      721 aaaaatacgg tcgcgcaaac aaactttctg gtctggataa ggcactggct gctggcgcca
      781 ctgaaatcac aaaagaagaa tattttgccc gaaaaaatgg cacatacacg ggcttaccgc
      841 aaaatgtaga taccgctgaa gattcagaac aaccagagcc gataaaagtt accgctgacg
      901 aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccgat aagttgcttg
      961 ctgcatcacg tggtgaattt gttgaaggga ttagtgaccc gaatgatccg aaatgggtta
     1021 aggggatcca gacccgcgat tctgtgaacc agaaccagca tgaatcggaa cggaactacc
     1081 aaaaagcgga acaaaacagc ccaaatgcgt tacaaaacga gccagaaacg aaacagcccg
     1141 aaccagtggc gcaacaggaa gtggaaaaag tctgcaccgc ctgcggtcag accggcggcg
     1201 gcaacggccc tgattgtggc gcggtgatgg gcgacgcaac ataccaagaa acattcgatg
     1261 aagagtatca ggttgaagtt caggaagatg atccggagga aatggaaggc gctgaacatc
     1321 cacacaagga gaacactggc ggcaatcagc atcacgatag cgataatgaa actggcgaga
     1381 cggcagatca ctcaattaag gtgaacggtc atcacgaaat cacatccacc agcaggacgt
     1441 gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc ccgatcatct
     1501 caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa tttagtaaga
     1561 ctatcgatct ggaaactgct ggcggagtca ttgatcggga caccattaaa tggtggctta
     1621 agcaatcacg cgaagcac
//
LOCUS       DAHBHC010000280         1609 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHBHC010000280 DAHBHC010000000
VERSION     DAHBHC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.285"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            325..815
                     /locus_tag="M2A35_005441"
                     /pseudo
     CDS             325..815
                     /locus_tag="M2A35_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1040..1531
                     /locus_tag="M2A35_005442"
     CDS             1040..1531
                     /locus_tag="M2A35_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCB7201568.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          611 a          261 c          249 g          488 t
ORIGIN      
        1 tgttgtttgc gtgagaacct tctcagcatt aatgaatttt ttaacggaat ccttggtttc
       61 tttttcgagt ccgaatttac aatatcatgc atatagacca tttcatgaat ctgcgaaaac
      121 tcaatgtcca ttgtatacct cacattttta ccgtgactcg atgttactgt tcaataatca
      181 ccttccatca atactaaaat taatacccct aatgtgccga taacaaatat agtcattcta
      241 cgtaacgtct ccataaggtg atatttgaca ttatcagaag ctgcgaattc ggattttgct
      301 ctaatcaaat gagagaaatg taaaatgaag ccacgaaata ttaataatag cctatcactg
      361 caaccattag ttcctgatca ggagaacaaa aataagaaaa atgaagagaa atccgttaat
      421 ccagttaaaa tcacaatggg gtctggttta aattatattg aacaagaatc tcttggagga
      481 aaatatctaa tacatgattt gtcaataaag atagcggata tttctgaaga gataattcag
      541 caagcaatat tatctgctat gagcatatat aaattttcga taacagatga tttaatgagt
      601 atggctgtaa atgaactcat aaaactgacc aaaatagaga ataatgtaga cctgaataaa
      661 ttcactacta tatgcacaga cgttctatcc cccctcgtca ccagacataa taaagaaaaa
      721 aaacaaacga cattctaccc ttcgcaaaaa tccccttttt aattttcatt gaaaaaatgg
      781 caattgatac tatgaatcca ggtcatacac attaaggcat ttatgtaatg tcgtaattaa
      841 gacaactaat aaggtgaata ttagtaaaac aactaatgat gttattattt tgtagtgaac
      901 agtactttta ccaataatga aaaatatacc ataagcaatg caagcttaaa accaataatt
      961 taaaaaagat aaatgtaaaa atattaatga gtcaatcaat aataatttag catcacgaga
     1021 cacatcacag aggaatatta tgagcacaga aacaattgaa atattcaata atagtgatga
     1081 atgggcaaat caactaaaac acctattatc gaaaggagaa aatctggcat tactacatgg
     1141 tttaactcct gatatccttg atagaatata tgcatatgca ttcgactacc atgaaaaggg
     1201 taatgtaacg gacgcagaaa tttattataa atttttgtgc atttatgcgt tcgaaaatca
     1261 tgagtatcta gaaggttttg catcagtatg tcagtcaaaa aagaaatatc aacaagcata
     1321 tgacctttac aaactaagtt acaattactt cccgtatgat gactattcag ttatttatcg
     1381 tatgggtcaa tgtcagattg gggctaaaaa tatcgataac gcaatgcaat gtttctatca
     1441 cattattaac aattgtgagg atgatagtgt taagagtaaa gcgcaggcat atattgaact
     1501 cttaaacgat aattcagaag ataatggcta agcattacaa tcatttggtt aaatttgctg
     1561 gttatagtgc agttaaccac tataaccagc acctcttatg ttttcttaa
//
LOCUS       DAHBHC010000281         1592 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHBHC010000281 DAHBHC010000000
VERSION     DAHBHC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.286"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..415
                     /locus_tag="M2A35_005443"
     CDS             <1..415
                     /locus_tag="M2A35_005443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201569.1"
                     /translation="VNDLNSVFSFSQTDVNNALNTFDDQLKDIVNSDDEDSTDDYDSL
                     TSGFIDKLPKEWLFIDFDKLFFTSEQQGAQPIEINFSFSLPVIGIVHFSIDTTDFSSA
                     YDLLLRPVLEYFIYVLTIFRLYFVYRRALMRHAEV"
     gene            418..780
                     /locus_tag="M2A35_005444"
     CDS             418..780
                     /locus_tag="M2A35_005444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201570.1"
                     /translation="MVVFIQFIYSFVLIIFRRLLPFLRNKFPSVFSYTGTLTYLFMSI
                     SLAVSFIVAANAVIDTVSSSAVMNRYLIVAFSYLPHNFTFCANLVLAADFISFLFHSK
                     DRIYRYIRLFISGKISGV"
     gene            784..>1592
                     /locus_tag="M2A35_005445"
     CDS             784..>1592
                     /locus_tag="M2A35_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201571.1"
                     /translation="MPVITGNLGQGKGIMAAYLASLYYRRGLRVAANYPLNTEFMSSG
                     SDNPVTVIPAMPRIEDLELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLIAQHEDMIDSQIIKAMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGIASGKRGIIPHYVTASTFLYDGTAHAARRPVDKVVIKAADYYNVYDTNFIFSDG
                     MELLNGRFVDMRAVYSVLPGRMLMTMNQLPEQSDKKAAPQKKPWGKLIAFL"
BASE COUNT          378 a          283 c          338 g          593 t
ORIGIN      
        1 agtgaatgat ctgaacagtg tattttcttt ttctcagact gatgtaaata atgcactgaa
       61 tacttttgat gaccagttga aagatattgt caatagtgat gatgaagatt caacagatga
      121 ttatgattct ttaacttctg gatttattga taagttaccg aaggagtggt tgtttattga
      181 ttttgataag ttatttttca cttctgaaca acaaggtgct caacctattg agattaattt
      241 ttcattttct cttcctgtga ttgggattgt gcatttttca attgacacga ccgatttttc
      301 atcagcatat gatctgttgt tgaggcctgt tcttgaatat tttatttatg tattgactat
      361 ttttcggctt tattttgttt atcgccgtgc tctgatgcgt catgctgagg tctagttatg
      421 gttgttttta tacagtttat ttattctttt gttcttatta tttttagaag attacttcct
      481 tttctcagga ataaatttcc ttctgttttt tcttatacgg gtacgttaac gtatcttttt
      541 atgtcgattt ctttggctgt aagttttatc gttgcagcca atgctgttat tgatacggtt
      601 tcttcttctg cggttatgaa cagatatctt attgttgcgt tcagttattt acctcataac
      661 tttacatttt gtgcaaatct tgttcttgcg gctgatttta tttcttttct tttccattca
      721 aaggatcgta tttatcgtta cattcgatta tttatatcag gaaaaatttc aggagtataa
      781 tttatgcccg ttattacagg gaatctgggg cagggaaaag gcattatggc ggcttatctt
      841 gcctcgcttt attatcgccg tgggttgcgg gttgctgcaa attacccgct taacactgaa
      901 ttcatgtctt ccggttcgga taatccggtt acggttattc ctgccatgcc gcgaattgaa
      961 gaccttgaac ttctcggacg tggatgccct gaaaatgaaa aaacacgctt cggggcgtta
     1021 tttcttgatg agtgtgcgac atggcttaat acccgtggct ttgcccgtaa ggaccgacta
     1081 ccgttaattg actggctgat acattcccgg aagctgggct gggatgttta tctgattgca
     1141 cagcatgaag acatgattga ttcccagata attaaggcga tgggggctaa aattattcgc
     1201 tgccgtcgtc ttgatgaatt gcgcgttcct gtgattacac cgctgatgga attattcaga
     1261 ccaggaaaaa cggggattgc atcggggaaa agggggatta ttcctcatta tgttaccgcc
     1321 agtacgtttc tttatgacgg caccgctcat gctgcccgac gtcctgttga taaggttgtt
     1381 attaaggccg ctgattatta caacgtttat gacaccaatt ttattttttc tgacgggatg
     1441 gaactgctta acggtcgttt tgtggatatg cgcgctgttt attccgtttt gccaggacga
     1501 atgcttatga ccatgaatca gttacctgaa caatccgata aaaaagctgc tccacagaaa
     1561 aaaccatggg gaaagttgat tgcttttctt tt
//
LOCUS       DAHBHC010000282         1564 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHBHC010000282 DAHBHC010000000
VERSION     DAHBHC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.287"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..562
                     /locus_tag="M2A35_005446"
     CDS             <1..562
                     /locus_tag="M2A35_005446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HCB7201572.1"
                     /translation="NFFNYLIGRKESIIHFSSARVAMSACPQDMMHILFNKFVHHSEI
                     TKESRETILTRELKHRFSHYNELRNAFKKEMLGGELTRFSVPFVARHDGEILCAIKPL
                     TFTQDKPEKMLEHCDSWTAKILRAASEKILSISNVLFTIDAPRQPSELEVKAMSEIRK
                     TFDEKGINHVEHQDEASIVKFAKQAI"
     gene            1002..1310
                     /locus_tag="M2A35_005447"
     CDS             1002..1310
                     /locus_tag="M2A35_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCB7201573.1"
                     /translation="MTVIVYGKSTFAGNAKTRRHERRRKLAIERDAICNIIDSIFGTD
                     SEEPVQKGTRKRLSLSEKAISLGNIRNQNTDECSGSICLPNVAIYAAGYRKSKQLTAR
                     "
     gene            1369..>1564
                     /locus_tag="M2A35_005448"
     CDS             1369..>1564
                     /locus_tag="M2A35_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201574.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLMAILTIPLVFWFMK"
BASE COUNT          505 a          339 c          331 g          389 t
ORIGIN      
        1 aaattttttc aactacttaa ttggcaggaa agagtcaatc attcatttta gctctgcaag
       61 agttgcaatg tctgcttgtc cacaagacat gatgcacata ctttttaata agtttgtaca
      121 tcattcagaa ataacaaaag aatctcgtga gaccatcctg acgagagagc ttaagcatcg
      181 tttctctcat tataacgaac tcaggaacgc ctttaaaaag gaaatgcttg gtggtgaact
      241 aacgcggttt tctgtaccat ttgttgcccg tcacgatgga gagattttat gtgctatcaa
      301 acccctaaca ttcactcagg acaagcctga gaaaatgttg gagcactgcg actcttggac
      361 ggccaaaatt ctgcgcgccg ccagtgagaa aattttatct atctcaaatg tgttatttac
      421 gatagatgca cctcgccagc caagtgagct tgaggtaaaa gcgatgagtg agattcgtaa
      481 gacattcgat gagaaaggta taaatcacgt agagcatcag gatgaagcat caatagtaaa
      541 gtttgctaag caagcaattt aacccggcct cagcgccggg ttttctttgc ctctcgatcc
      601 cccttcccaa caacacataa ccatttgtat ttattggaaa actaatagat acaacttgct
      661 aaacagtgca attctgatcc ctcacctacc taacaattca cccctgcaaa aaataaatcc
      721 atataaaaaa catacagata accatctgcg gtgataaatt atctctggcg gtgttgacat
      781 aaataccact agcggtgata ctaaacacat cagcaggacg ctggaagcca aacggaacag
      841 attggcatgc tctttaactt cgatggggcg ctgacaaagc gcaaacagat accaaacgag
      901 atgggtttgg cggtgatgtg aattgcagct gcaacgacag caaccagaag atcagcatct
      961 ggcgcatcac caccaaagcc atttcacatg aggaaaacat catgacggta atcgtgtacg
     1021 gaaaatcaac atttgcagga aatgccaaaa ctcgccgtca tgagcggcgc agaaagctgg
     1081 ccatcgagcg tgatgctatc tgtaacatca ttgattctat cttcggcaca gacagtgagg
     1141 aacctgttca gaaaggcacg agaaagcgtt taagcctttc tgaaaaagca atatcactcg
     1201 gcaacattcg taaccaaaat accgacgaat gcagtggaag tatttgcctg ccaaacgtag
     1261 ctatttacgc ggcaggctac cggaaatcaa aacaactgac ggcgaggtga cttgtgttgg
     1321 tcgccagaaa atgaaattag gcagcaaacc acttatttga ggtgatatat ggaagaacaa
     1381 gcaaacaaga ttctcgtaga actactgcaa aaagccagta atggaataga cgcggctgtt
     1441 tcatttagcc aggcacagat tcctgatgtt gttcatcagt tgctgctatg gaatatggtt
     1501 gacagtctga ttaaaacatt aatggccatt ctaacaatcc cactggtttt ctggtttatg
     1561 aaga
//
LOCUS       DAHBHC010000283         1550 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHBHC010000283 DAHBHC010000000
VERSION     DAHBHC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.288"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..757
                     /locus_tag="M2A35_005449"
                     /pseudo
     CDS             1..757
                     /locus_tag="M2A35_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386197.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            835..1550
                     /locus_tag="M2A35_005450"
                     /pseudo
     CDS             835..1550
                     /locus_tag="M2A35_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          392 a          383 c          515 g          260 t
ORIGIN      
        1 aataaaaccc tggttctttc cggtaatcag tccggactga ctgccgatcg catgctgacg
       61 ctctccagag ccggacaggc cgcagggctg acgtttaacc aggcgagtga gtcactggca
      121 gccctggtga atgccggtgt gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc
      181 gcgcgttttg cttctgcatc cggtgtggag gtggacaagg ttgcagaggc tttcggaaaa
      241 ctgaccaccg actctacgtc ggggctgatt gcgatggcgc gccagttccg taacgtgacg
      301 gcagagcaga ttgcgtatgt tgcgcagctg cagcgttccg gtgatgaggc cggggcctta
      361 caggcggcga acgatatcgc cacgaaaggc tttgatgagc agacccgtcg cctgaaagaa
      421 aacatgggga cactggagac ctgggcggat aaaaccggga aggcattcaa atcgatgtgg
      481 gatgccattc tggatatcgg tcgtcctgag tcctcagcgg atatgctcgc cagtgcgcag
      541 aaggcatttg atgaggcgga taaaaaatgg cagtggtacc agagccggag ccagcgccgc
      601 ggtaaaacct cttctttccg ggccaacctt cagggcgcat ggaatgaccg ggaaaatgcc
      661 cggctggggc tggcagcggc cacgctgcag tcggatatgg aaaaagccgg tgaactggcc
      721 gccagggacc gggccgaacg ggacgcatca cagctgaagt ataccggaga ggcgcagaag
      781 gggtatgagc gtctgctgac gccgctggag aaatataccg cccgtcagga agaactgaat
      841 aaggccctga aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg
      901 aaaaaggatt atgaatcgac gctgaaaaag ccgaagtcgt caggagtcaa agtgtcagcc
      961 ggtgagcgtc aggaagacca ggcgcatgct gccctgctgg cgcttgaaac cgagctccgg
     1021 acgctggaaa aacacagcgg tgcgaatgag aaaatcagcc agcagcgtcg cgatttatgg
     1081 aaagcggaaa atcagtatgc ggtcctgaaa gaggcagcca cgaaacggca gttatctgag
     1141 caggaaaaat ccctgctgac ccatgagaaa gagacgctgg agtacaaacg ccagctggct
     1201 gagctgggag acaaagttga acaccagaaa cggctgaatg agctggcaca gcaggctgcg
     1261 cggtttgaac agcagcagag tgcgaagcag gcggcaatca gcgcaaaagc ccgcggactc
     1321 accgaccgtc aggcgcagcg ggagtcggaa gagcagcgcc ttcgtgacgt gtacggtgat
     1381 aatccggatg cgctggcgaa ggccacatct gcactgaaga acacctggtc tgcggaggag
     1441 cagcttcgtg gaagctggat ggccggtctg aagtccggct ggggcgagtg ggcagaaagt
     1501 gcgacggaca gtttttcgca ggttaaaagc gtggccacgc agacctttga
//
LOCUS       DAHBHC010000284         1529 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHBHC010000284 DAHBHC010000000
VERSION     DAHBHC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.289"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            24..1520
                     /locus_tag="M2A35_005451"
     CDS             24..1520
                     /locus_tag="M2A35_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCB7201575.1"
                     /translation="MRSALARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRRE
                     VEQAEALLKQREQDSDRCSGREDDPQVPRQLRQSRHRRPLPAHLPREIHRLESEESCC
                     PECGGELDYLGEVSAEQLELVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIA
                     GPGLLARVLTGKYCEHLPLYRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYR
                     YVMNTRKLHTDDTPVKVLAPGLKKTKTGRIWTYVRDDRNAGSSSPPAVWFAYSPNRQG
                     KHPEQHLRPFRGILQADAFTGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSA
                     TAEEALKRISELYAIEDEIRGLPESERLAVRQQRSKALLTSLHEWMVEKNGTLSKKSR
                     LGEAFSYVLNQWDALCYYSDDGLAETDNNTAERALRAVCLGKKNYVFFGSDHGGERGA
                     LLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
BASE COUNT          388 a          389 c          455 g          297 t
ORIGIN      
        1 tggttgaaga gacggcggcc ttcctgcgct ctgcactggc acgtgccgaa gaaaagatcg
       61 aagaggatga acgggaaata gaacatctgc gggctcagat agaaaaactg cgccggatgc
      121 tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga acaggctgag gccctgctga
      181 aacaacgcga acaggacagc gatcgttgca gtgggcggga agacgatcca caggttcccc
      241 gccagttgcg acagtcgcgc catcgtcgtc cgttaccggc acaccttccc cgtgaaatac
      301 accgtctgga gtctgaagaa agctgttgcc cggagtgtgg cggtgagctg gattatctgg
      361 gggaagtcag tgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcacag
      421 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcgccgtccc
      481 gcccgataga gcgtggtatc gcgggccccg gattacttgc ccgcgtgtta acgggaaaat
      541 actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgacag ggtgtcgaac
      601 tgagccgtgc attactctcc aactgggttg acgcgtgctg ccagttaatg acaccgctga
      661 atgatgccct gtaccgttat gtgatgaata cccgcaagct tcacactgac gacacaccgg
      721 taaaggtact ggcaccgggc ctgaaaaaga cgaaaacagg gcgcatctgg acgtatgtcc
      781 gggatgatcg caatgcgggt tcgtcatctc ctccggcggt ctggttcgcg tactcaccga
      841 accggcaggg gaaacacccg gagcaacacc tccgcccctt ccggggtatc ctgcaggcgg
      901 atgcgttcac aggttatgac aggctgttca gtgcagaacg tgaaggtggt gcgctgacag
      961 aagttgcgtg ctgggcccat gcccggagaa aaatccacga tgtatacatc agcagcaaaa
     1021 gtgcgacggc agaagaagcc ctgaagcgaa tcagtgaact gtacgccatc gaggatgaaa
     1081 tacggggatt accagagtca gagcgtcttg cagtcagaca gcagcgaagc aaagcgttac
     1141 tgacgtcgct gcatgaatgg atggtggaga agaatggtac gctgtcgaaa aaatccagac
     1201 tgggcgaagc gttcagctat gtactgaatc agtgggacgc cctctgttat tacagtgatg
     1261 acggactggc ggagacggac aataacacag cggaaagagc gcttcgtgca gtctgtcttg
     1321 gaaagaaaaa ttacgtgttc ttcggtagcg atcacggcgg cgagcgtggt gcactgctgt
     1381 acgggctgat cggcacctgc cgtctgaacg gtatcgatcc ggaagcgtat ctgcgccata
     1441 ttctgagcgt actgccggaa tggccctcca accgtgttga cgaactcctg ccatggaacg
     1501 tagttctcac caataaataa gcgtcaata
//
LOCUS       DAHBHC010000285         1526 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHBHC010000285 DAHBHC010000000
VERSION     DAHBHC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.290"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..708)
                     /locus_tag="M2A35_005452"
     CDS             complement(<1..708)
                     /locus_tag="M2A35_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201576.1"
                     /translation="MPVITGNLGQGKGIVAAYFASLYYRRGLRVAANYPLNTECMSSG
                     SDNPVTVIPAMPRIEDLELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLIAQHEDMIDSQIIKAMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGIASGKRGIIPHYVTASTFLYDGTAHASRRPVDKVVIKAADYYNVYDTNFIFSDG
                     MELLNGRFVDMRAVYSVL"
     gene            complement(726..1085)
                     /locus_tag="M2A35_005453"
     CDS             complement(726..1085)
                     /locus_tag="M2A35_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201577.1"
                     /translation="MWAILGAFFVEIVLKSLRFIAGLIGDKAVAWARLISYLGLCYTS
                     YYALITAVNFIIRQISVVNKIDVITIAFSYLPLNIQDCFNIILHVQFISYIYLYKDRV
                     YRLIYDLFKSNGLSVGK"
     gene            complement(1085..>1526)
                     /locus_tag="M2A35_005454"
     CDS             complement(1085..>1526)
                     /locus_tag="M2A35_005454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201578.1"
                     /translation="SVNDLNNIFSVSQSDMDNALNSLDNGIENINKNMVSSLPEGDFP
                     LSDLSQYLPGQGSENYDDSNWLTIDLDKLLNTNNGIALPFVLQFSFNLPLIGVIGFKV
                     DTTDFAKAYDYYVRSILEYTLYMMTMLRVFVITRRTLFEREVKS"
BASE COUNT          554 a          332 c          263 g          377 t
ORIGIN      
        1 taaaacggaa taaacggcgc gcatatccac aaaacgaccg ttaagcagtt ccattccgtc
       61 agagaaaata aaattggtat cataaacgtt gtaataatca gcagctttaa taacgacctt
      121 atcaacggga cgtcgggaag catgagcggt gccgtcataa agaaacgtac tggcggtaac
      181 ataatgagga ataatccccc ttttacccga tgcaatcccc gtttttcctg gtctgaataa
      241 ttccatcagc ggcgtaatca caggaacacg taattcatca agacggcggc agcgaataat
      301 tttagccccc atcgccttaa ttatctggga atcaatcata tcttcatgct gtgcaatcag
      361 ataaacatcc cagcccagct tacgggaatg tatcagccag tcaattaacg gtaatctgtc
      421 cttacgggca aaaccacggg tattcaacca tgtcgcacac tcatcaagaa ataacgcccc
      481 gaagcgtgtt ttttcatttt caggacatcc acgaccgaga agttccaggt cttcaattcg
      541 cggcatggcg ggaataaccg taaccggatt atccgaaccg gaagacatgc actcagtgtt
      601 aagcgggtaa tttgcagcaa cccgcaaccc acggcgataa taaagcgagg caaaataagc
      661 cgccacaatg cctttaccct gacccagatt acctgtaata accggcatgt tttatccccg
      721 catgattatt taccaacaga aagtccattt gatttaaaca aatcataaat caaacgatac
      781 accctgtcct tataaagata tatgtaagag ataaattgaa catgaagaat gatattaaaa
      841 caatcctgaa tgttcagagg taagtaacta aatgcaattg ttataacatc aattttatta
      901 accacggaaa tttgacgaat aataaaatta accgcagtaa taagtgcata atatgacgta
      961 taacaaagac caagataaga aataagtctg gcccatgcga cagccttatc gccaatcaat
     1021 ccggcaataa aacgtagaga ttttaagaca atctcaacaa aaaaagcgcc taaaatagcc
     1081 cacatcagga tttaacctca cgttcaaaaa gtgtacgacg cgtaatcaca aaaacacgaa
     1141 gcatggtcat catataaagg gtgtattcaa gaatggagcg aacataataa tcataagcct
     1201 ttgcaaaatc agttgtgtca accttaaaac caatcactcc aattaaagga aggttaaaac
     1261 taaattgcaa aacaaaagga agtgcaatcc cattgtttgt attcaatagt ttatccaaat
     1321 cgattgttaa ccaattacta tcatcataat tttcacttcc ctgaccaggc aaatattgag
     1381 aaagatcact taaagggaaa tcaccttcag gtaaagacga aaccatattt ttattaatat
     1441 tctcaatacc gttatccagt gagtttaaag cattgtccat atctgactga gaaacagaaa
     1501 aaatattatt cagatcattc actgaa
//
LOCUS       DAHBHC010000286         1522 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHBHC010000286 DAHBHC010000000
VERSION     DAHBHC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.291"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            97..780
                     /locus_tag="M2A35_005455"
     CDS             97..780
                     /locus_tag="M2A35_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCB7201579.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            781..1002
                     /locus_tag="M2A35_005456"
     CDS             781..1002
                     /locus_tag="M2A35_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201580.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            1014..1448
                     /locus_tag="M2A35_005457"
     CDS             1014..1448
                     /locus_tag="M2A35_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCB7201581.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
BASE COUNT          356 a          416 c          439 g          311 t
ORIGIN      
        1 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc acaggggtgc
       61 cctcccggtg ccctctgaga aagaaggagt gttgttatgt cccgttttgt cctcggtaac
      121 tgcattgatg ttatggcgcg tattcctgat aacggcattg atttcatact gactgacccg
      181 ccatacctcg tcggttttcg tgaccgacag gggcgtacca tcgccggcga taaaaccgat
      241 gaatggctgc aaccagcctg taatgaaatg tatcgcgtac tcaaaaaaga tgcgctgatg
      301 gtcagcttct acggctggaa ccgcgtcgat cgctttatgg ccgcctggaa aaatgcggga
      361 ttcagtgtgg ttgggcacct ggtcttcacc aaaaactaca catcgaaggc cgcatatgtg
      421 ggttatcgcc acgaatgcgc ctacgttctg gcaaaaggcc gtccggcgct gcctcagaag
      481 ccattacccg acgtgctggg ctggaaatat tcaggcaatc gtcatcaccc gacggaaaag
      541 cctgttacca gcctgcaacc actgattgag agcttcacac atccgaacgc gattgtgctg
      601 gacccgtttg caggcagcgg ctcaacctgc gtggctgccc ttcagtccgg acgccggtat
      661 atcggtatcg agctgcttga gcagtatcac cgtgccggac agcaacgtct ggctgccgta
      721 cagcgggcca tgcagcaggg ggccgcaaat gatgactggt ttatgccgga ggctgcgtaa
      781 atgaactatg ccggacatga aaaacttcgc gccgaagtgg cggaggtggc caatgccatg
      841 tgcgacctgc gtacaaccat gaatgagatg gagcggcggt acagctttaa tgctgacacc
      901 ctgccggaac gtctggtgcg tcagacgttg tttcgcgcaa accgcctcct gatggaggca
      961 tataccgaaa ttctggaact ggattcctgc ttcaaagatt gaggggagag agtatgtacg
     1021 gaacatgcga aatactctgc cgggagctgg cagcaaagta tccggcagac acgccactga
     1081 tgctggttgt ctggtccccg gaagagattc aggccctcgc tgacggaatg gatatttccc
     1141 tgtccgatca tgaaatcagg accgtcctgg cgcgcctgga ggacatcccg gaagaccagc
     1201 ggattgagtc cggtatttct tccggcgtgg cgatggagat tatcagcaac gtgagagaaa
     1261 accgccaggt gaccgtccct gctgaactgc tggcgtccct gattcagacc gctgaacagg
     1321 cactgtggaa acgtgaatgg gccgcccgtg ataacggcct tgccgtcccg gaatgtgtta
     1381 cccgccgtca ggcagtgatt aatcaggccc gcaccctgct gaaaaacaac acacacgaaa
     1441 acaactgatg ttatcgccgc ctgccctgcg ggcggcgata cagggagaac gcttaatcat
     1501 gaacaaaacg ctgaatgcac tg
//
LOCUS       DAHBHC010000287         1466 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHBHC010000287 DAHBHC010000000
VERSION     DAHBHC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.292"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            33..224
                     /locus_tag="M2A35_005458"
     CDS             33..224
                     /locus_tag="M2A35_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="partitioning protein"
                     /protein_id="HCB7201582.1"
                     /translation="MAFLAGLALLKMNPAEAYRLAAWADDEALSVTQTRTECPASQPV
                     STAQITSQMAGNIRALFPE"
     gene            323..730
                     /locus_tag="M2A35_005459"
     CDS             323..730
                     /locus_tag="M2A35_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201583.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQTKPLNNIHEKYDYFTDTACLLTSEEQKIAQLLGDAWNLYLTLPVEHPMG
                     RDEFCRAIHHCQNMVLARPAIRALARKGQGYKR"
     gene            complement(1215..>1466)
                     /locus_tag="M2A35_005460"
     CDS             complement(1215..>1466)
                     /locus_tag="M2A35_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCB7201584.1"
                     /translation="DEFMNVKAASRDDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARV
                     YAINELMPVMEAMKHINDWLGEEVIRFNPYALLDTQPTS"
BASE COUNT          421 a          357 c          380 g          308 t
ORIGIN      
        1 ttacaccaga agcccgcaaa agcggccaac gcgtggcttt tctggccggg ctggcacttc
       61 tgaaaatgaa tccagcagag gcttaccgac tggctgcatg ggctgacgat gaggcgttat
      121 cagtgacaca aaccaggaca gaatgccccg cgtcacagcc agtatcaacc gcacagataa
      181 ccagtcagat ggccggaaat atccgggcgt tatttcccga ataacacaac atcagggcgc
      241 atccgccctg atgactttaa tccgggaaca taaacaaggg ggacacaatg caacacattg
      301 acagagaaaa aggaaaaaaa gcatgaacag aaaacagaaa caagagttga aatacttctt
      361 acgtaaagaa atagccaggc ttgaagatgc agagtcacaa tcatcagaaa ttccgttcgg
      421 aatggatatc aacgacgccc gtatgcttca ggcataccgt atagctcagg ctgcactaca
      481 gacaaaaccg ttaaataaca tacatgaaaa gtatgattat tttacggata ccgcttgctt
      541 gctgacatct gaagaacaaa agatagccca acttttaggc gacgcatgga acctgtattt
      601 aacattgcca gttgagcatc caatgggtag agatgaattt tgccgggcaa ttcatcattg
      661 ccaaaatatg gtgttggcac gcccggccat cagggcgctg gccaggaagg ggcaaggcta
      721 taaaagataa gtaaaacgcc ctcctccaga gcgtttattt caatgcacaa tagtgcacaa
      781 atttgcacga tttttttgaa cgactttttg cctttccggc ccgcgtggcg gctggatccg
      841 tcaaggatcc gtgcgtgcac aaaaaaacgc gctttttctg cgcgcaggtg acgggggaac
      901 agcccgcgtt tcagggggta agtagcatcc cctgaacgat gtcgcagcga cacaacagaa
      961 tggctgtatt tctcacgctg agcgtgaaaa agacgtgagg gcttttgatt tgatggggtg
     1021 gaagataagg ccgtcaaaat cgcactgagg cggcgagaac atgcagtcag cgcagtggga
     1081 ttgcgtaaga gtctgactgt cgatgatggc aataagcagg aaagcgtcgt gaaattatct
     1141 gattgataca agagctggag agtcggggca taaatttttt atgccccggc gaagcagcag
     1201 acaagcgaag cgcgtcagga tgtgggctgg gtgtccagca gtgcgtaagg gttaaagcgg
     1261 atcacctctt cgccaagcca gtcattgatg tgcttcatag cctccatgac gggcatcagc
     1321 tcgttaattg cgtaaacccg cgcggccttc tccacatcac caaacgcact tttttcgccc
     1381 ggcatcgccc ccatcagttg cggcggaacg cggtgcgcag ccagcacatc atcacgggat
     1441 gccgccttaa cattcatgaa ctcatc
//
LOCUS       DAHBHC010000288         1457 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHBHC010000288 DAHBHC010000000
VERSION     DAHBHC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.293"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(32..1081)
                     /locus_tag="M2A35_005461"
     CDS             complement(32..1081)
                     /locus_tag="M2A35_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7201585.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQTFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            complement(1083..1361)
                     /locus_tag="M2A35_005462"
     CDS             complement(1083..1361)
                     /locus_tag="M2A35_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201586.1"
                     /translation="MARNAKYYNSDNSPILACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
BASE COUNT          339 a          383 c          379 g          356 t
ORIGIN      
        1 taaggcttca aggttcatgc tcgttctcca cttacgccag tacgccaatt gccagcgcgc
       61 gatcgataaa acgaaatatc agctccagtt gggagccata cttatcttca aatgccactg
      121 tatccgtatg cagctcgttg tgatgctttc tgcacaaagg caacacaaag agatcatgtg
      181 cttttgttcc cattccgccc tgcccgtgac caatcagatg atgcggatcg tcggctggca
      241 taccgcagca agcacacggc tgtgtcttaa cccaacgtgt gtatttctcc ttaacccagc
      301 ggcgacgttt aggcagcttc atgaaagatt ccggagactc tggatcaacg gtgatgctta
      361 ccaccgtctt ttcctgtggt gatttttgtt gctggtgggc gtaaggcaac ggtgcaagat
      421 tttttgtgcg ttgtttcaat atgctggtgg cggtctgctc tcccggtacg atgtcgcttt
      481 cgcggtacac cgagcagatt ttttccgctg gtaatcccag cgaacgacgc gatacagcct
      541 caggtagtgc atccaccacc tgattgcaga ccgcccacca ggataattca gccaaagata
      601 attcccgctc ctgcgtaccg cttattgcgt gacggatgac gtcaatcacc catgctgtca
      661 gattttgttg agcaagcagc tccagtgatt ccgatgtctg gtcacgcagt tggttgtcgc
      721 agtgccagca caacaccatt gcgccggtac cataacggtg aatgactgtt tcagtgtgat
      781 ggtaatcgcc attaggccac tggcaggatg taacatgacg taatagccag tcggacaatg
      841 cgccaacgcc gccagcagca cgaatcaccc gttcgttact aaaaaacggc agcaatgttt
      901 tgtcttccgc cagcggctgg cgaacggcag gaacgactcc ggatggcaga ttacgcatgc
      961 tttttggttc cggttccacc agcactcgag gattatgaaa tgtctgtatg gattcacggc
     1021 ccggcttaag gaccaccagc ccaagctcag gcaccagaac aggtctaagt aatacccgca
     1081 cgttacctcc agatccgttg ctggaaagtg cgggacgcac gtggtgggcg ttcggaataa
     1141 ggcagcctga cagagattat ccagtgccga tagtcgagac tgagagcttt cttaacctcg
     1201 aacccgcgcc tgcggtaaga atgaatcagc cattcggcct gttctgcagt gcatggaggg
     1261 tgctggaacc attcagactt gaatgcgtga gaataccgcc cgtgcgtgca ggcaagaatg
     1321 ggcgaattat cagaattgta atattttgcg ttgcgtgcca tcggttttct ccggtggcac
     1381 ggtgttactc agcgggagtt cagccccgcg caagattgta gatgagttta ttctcctgaa
     1441 aaagcagaaa agccagc
//
LOCUS       DAHBHC010000289         1443 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHBHC010000289 DAHBHC010000000
VERSION     DAHBHC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.294"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..440)
                     /locus_tag="M2A35_005463"
     CDS             complement(<1..440)
                     /locus_tag="M2A35_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCB7201587.1"
                     /translation="MSVRSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDQRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALV"
     gene            complement(437..871)
                     /gene="psiB"
                     /locus_tag="M2A35_005464"
     CDS             complement(437..871)
                     /gene="psiB"
                     /locus_tag="M2A35_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCB7201588.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHQRFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFASEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(926..>1443)
                     /locus_tag="M2A35_005465"
     CDS             complement(926..>1443)
                     /locus_tag="M2A35_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201589.1"
                     /translation="HYSLTSDAPSGKEGAAFMALMAEKARLVALLPEGWSRDMTTFLS
                     LSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDPLETAIGFHMRDWWQPTKANFFG
                     HLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGWMCAPRPQTDA
                     TERTDNLADAA"
BASE COUNT          277 a          451 c          399 g          316 t
ORIGIN      
        1 accagtgcct ggtacgcacg ggctttctgc tctgcctccc gtgcctgctg acggctgtac
       61 ttctgcatga ccagtgcgga tttgtggcga ctgcgctccc tgacacggaa ccgcaccgac
      121 ggaaacaacc gctctgctgc atcaccggga aggggaagga ggcagacctc gccctgtgtt
      181 tcaatcagca catcgatggc atacagccac tgaagacggt tgccgtgcag ttcttctgcg
      241 gtcagggacg gcatgaaaaa tcgctggtca gacagcgata tcctgcggct tccgttcaga
      301 cagcggaaaa acgcccccgc atacggatat tccgccagcg tgttaccctg atggcggcgt
      361 ttttctgtct ccgccaccgc ccgccatgcg gcctgttgct ctgtgctgag gggaaccagt
      421 gcctgtgaac gtacgctcat acctgacctc cttccgccat caggatatgg atgatgtcgt
      481 taacgctgta cccctgcgta tccagtgacg ccgccagtgc cagtgaatgg ctgacggctt
      541 ccgatgcaaa ccgccgctgt acctgaacca ccgcaaacgg ctcaccaccg gcattcacca
      601 gaaccagcac ccagacctga gagacatcgc ccggtgaaca taacgccagg tgaaaacgtt
      661 ggtgggcagg cgtgaaacgg acctggacgg ggaaaaagcc gccaaactcg ctgccgtact
      721 cgccgttcat cgtccagtta tccggcgcat ccagctcccg catcacggcg tgagtcagct
      781 cacggcgttc atcacttccg gcagcccgga tatcttcata ttcctgtgcg ttcatggtct
      841 gtaatacatt cagggtcagt tcagttttca tcacggtatc tcccttgctg ccgccccgtc
      901 tccggcgggg cggtgtggtt gttcatcagg cggcatcagc caggttatcg gtacgttcag
      961 tggcatccgt ctgtgggcgt ggtgcacaca tccagccagg cacccagcgg ttgtctttca
     1021 tgtggaactc tgcatgttca gccgcatcgc ctttcttcat cttctccgcg tcccgtgcag
     1081 caccggacag tccggcctca ttcagggctg cgataatctg cggctttttc aggtgtccga
     1141 agaagtttgc ttttgtcggc tgccaccagt cgcgcatgtg gaatccgata gcggtttcca
     1201 gcgggtcaag cggactgcgt gacgtgtgac cacactcgcg ggtctgaacg ccgtggatgc
     1261 tgcacgcagt gcagaaactg agcagggata acagcacctc ctggctgagt gacaggaacg
     1321 tcgtcatgtc ccgtgaccat ccctccggca acagggcgac caaacgtgct ttttctgcca
     1381 tcagcgccat gaatgcggca ccttccttcc ctgacggcgc atcgctggtc agcgaataat
     1441 gtt
//
LOCUS       DAHBHC010000290         1437 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHBHC010000290 DAHBHC010000000
VERSION     DAHBHC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.295"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..575)
                     /locus_tag="M2A35_005466"
     CDS             complement(<1..575)
                     /locus_tag="M2A35_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCB7201590.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDARHSNTSMAW
                     GAGVQFNPAESVAVDVAYEGSGSGDWRTDGF"
     gene            complement(643..>1437)
                     /locus_tag="M2A35_005467"
     CDS             complement(643..>1437)
                     /locus_tag="M2A35_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCB7201591.1"
                     /translation="EQTKDGKHYVAGIGLSMEDAEEGKLSQFLVAANRIAFIDPANGN
                     ETPMFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNAN
                     AGTLNNVTINENCQIKGKLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDD
                     QPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVID
                     MPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          326 a          419 c          346 g          346 t
ORIGIN      
        1 atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt cataagcgac atcaacggcc
       61 acggattcgg ccgggttaaa ctgcacgcca gctccccacg ccatagaggt gttactgtgg
      121 cgagcgtcat cacttccggt cagcacgtcg tgcgttttcc ccttgttgtc agttacgcgg
      181 agataatccc cggagaaagt cgacacacgg ctgtaagcca cacccgccat cgcatacgcg
      241 ctgaaccatt cattcacgcg tacagacggc cccgccatca cgctgaacca gcggttacgc
      301 acggaatctt catgccagcg ggtatcgctg taatgggtca gctggcgatt cttgtctcct
      361 gcatagctga acgacgtcac catccccagt gtgtccgtaa actcataacg gtatttcacg
      421 ttaatcccgt tcagttcatc gctgccagga acgttcgtcg agacatgaag ataccccgcg
      481 ctcagcgtgg actgatgttc agacgcccat gcaggcgcac cggatacggc cagacaaatg
      541 gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata
      601 aaaaaggcgc catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc
      661 tgcggtggct ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca
      721 gttattcact agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg
      781 catatcaata acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc
      841 agcaccgttt ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc
      901 atgcttagcg ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc
      961 gtcataaacc ctgacggtaa tggtccctga tggccaccgc tccggtgccc gggagtcccg
     1021 cggaaaagcc ttacccactg ttttgactat atcgccttca atctggttgg ctgacagttt
     1081 ccccttaatc tgacagttct cattaattgt gacattgttg agcgtcccgg cgttcgcatt
     1141 cacactgcca ctgatatctg catttttagc ggtcagcttt ccgtccggtg tcagggaaaa
     1201 tgccggagga ttaccgccgc tggtaatggt gggagccgtc aggcgcttca ggaacacgtc
     1261 gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg
     1321 gtcaataaac gcgatacgat tggcggcaac cagaaactgg ctcagtttgc cttcctccgc
     1381 gtcctccatg ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctc
//
LOCUS       DAHBHC010000291         1419 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHBHC010000291 DAHBHC010000000
VERSION     DAHBHC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.296"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            143..403
                     /locus_tag="M2A35_005468"
     CDS             143..403
                     /locus_tag="M2A35_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201592.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTGGFILRENEFVTSLPSLKEAELKAGFSAFEDE"
     gene            470..748
                     /locus_tag="M2A35_005469"
     CDS             470..748
                     /locus_tag="M2A35_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201593.1"
                     /translation="MAHDTKLHNSDDSAVFASWHGRRSRTFKSDWFQHDPCTEEQAEW
                     LIQNYRRRGYEFQKDLSLDSRHWIISVRLPYSERPPRSSRTYQQRIWR"
     gene            750..>1419
                     /locus_tag="M2A35_005470"
     CDS             750..>1419
                     /locus_tag="M2A35_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCB7201594.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMQVFHNLRVLVEPEPKRMRN
                     LPSGVVPAVLQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SEIVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWVIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSVYRESDIVPGEQTATSILKQR
                     TKNLA"
BASE COUNT          338 a          340 c          386 g          355 t
ORIGIN      
        1 ggcgggtttt tgtttttatt ttcaacgcgt ttgaagttcc ggacggcgcc ggaatagaat
       61 caaaaatact taagtagcgc gcagggagaa gagggatgga ccccgaacag gggagtgcta
      121 tttatctgga aggattctgt tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga
      181 tggcctctat actgtgcgcg ttgagaatgg cgctattgtc agccactgcc gtattaaatg
      241 tttacagtct caacaaagga agagtggagc tgcgttaatt cattttgtgg atgggcttgt
      301 gacgggtggt tttattctgc gtgaaaatga atttgtcaca tcattgccgt ctctgaaaga
      361 agctgagctt aaggctggtt tttctgcttt tgaagatgag tgaattcatc tacaatttag
      421 cgcagggctg aacccctgtt gagtaacact gtgccaccgg agaaagccga tggcgcacga
      481 taccaaatta cacaattctg atgattctgc cgtctttgcc agctggcacg ggcggcgctc
      541 ccgcactttc aaatctgact ggttccagca tgacccatgc actgaagaac aggccgaatg
      601 gctgattcag aactaccgca gacgtggtta cgagtttcag aaagacctca gccttgactc
      661 ccgacactgg ataatctccg tcaggcttcc ttactccgaa cgcccaccgc gttcatcccg
      721 cacataccag caacggatct ggaggtaacg tgcgggtatt acttagacct gttctggtgc
      781 cagaactcgg gctggtggtc cttaagccag gccgtgaatc catgcaggta tttcataacc
      841 ttcgagtgct ggtggagcct gaaccgaaaa ggatgcgtaa tctgccgtcc ggggtcgttc
      901 ctgccgttct ccagccgctg gcggaggata aatcattact gccatttttc agcaatgagc
      961 gtgtgattcg tgctgctggc ggcgctggtg cactgtctga ctggctgttg cgtcatgtca
     1021 aatcctgcca gtggcctcat ggtgactatc atcacagtga aatcgtcata catcgttatg
     1081 gtaccggcgc gatggtgttg tgctggcact gcgacaacca gttgcgtgac cagacctccg
     1141 aatcactcga gcaacttgct caccaaaact tgtcagcatg ggtgattgac gtcatacgcc
     1201 atgcaatgaa tggcacgcag gagcgggaat tatcgctggc tgaattatcc tggtgggcgg
     1261 tctgcaatca agtggcggac gcgcttccag aggcagtatt acgtcgttct ctggggttac
     1321 gtgcggaaaa aatccgctcg gtgtaccgcg aaagcgacat cgtaccggga gagcagaccg
     1381 ccaccagcat actgaaacag cgcacaaaaa atcttgcgc
//
LOCUS       DAHBHC010000292         1374 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHBHC010000292 DAHBHC010000000
VERSION     DAHBHC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.297"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            186..698
                     /locus_tag="M2A35_005471"
     CDS             186..698
                     /locus_tag="M2A35_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201595.1"
                     /translation="MCYGLELREIEQYPIELAIRMDSCGFLIFDSKLSCQVVDTLNSA
                     VALYHVGMRNHADKLTRVGVELLLNDFFEEIISNHEKDKKIKDALSLSQKEKACKPRN
                     HYYNEVMHVIKLTWEKYPKASKTGLLNELSVYYHGKVSKNTLDKWIKESGAQPPRPDK
                     YSSFELVHPQ"
     gene            841..1050
                     /locus_tag="M2A35_005472"
     CDS             841..1050
                     /locus_tag="M2A35_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201596.1"
                     /translation="MNLKKSDLSTDRLTRAEAAAYLGVNNQTLANWACTGKVKIPFHK
                     LGRKVLYMRTDLDAYLASTRRTQTV"
BASE COUNT          440 a          271 c          278 g          385 t
ORIGIN      
        1 cggtaaaaca atatattatt tattcggtta tgaatgattt aattactggt gggcgagact
       61 cttcagtttt aaacgttcct gtatctttat ccccgaaaaa ctccaccata gaggctataa
      121 aacgcttgat tgatgatata agagattatt tttataaatc aaagtatcca acaagagaat
      181 ttcttttgtg ttatggttta gaattaagag aaatcgagca atatcctatt gaattagcta
      241 taagaatgga ttcatgtggt tttttaatat ttgatagtaa actctcttgt caggttgtcg
      301 atactttaaa ttcagcagtt gcattgtacc atgtcggaat gcgtaatcac gcagacaaac
      361 ttactagggt tggtgttgag ttattgctta atgacttttt tgaagaaatt atatcgaacc
      421 atgaaaaaga taaaaaaata aaagatgctt tatccttatc acagaaagaa aaagcgtgca
      481 aaccaagaaa tcattattac aatgaagtta tgcacgtaat taaactaaca tgggaaaagt
      541 accctaaagc cagtaaaaca ggtttattga acgagctttc tgtttactat catgggaaag
      601 taagtaaaaa cactctggat aaatggatta aagaatcagg agcacaacca ccaagacctg
      661 ataaatattc ttcgtttgaa cttgttcatc cccaatagct ggctagtgta tcttaatagc
      721 tggctattaa ggctaaataa ctggtaacct ccccacagca actggctatt gggctaaaaa
      781 caaaataccc attgattgat atttatcacc gttgttactt agaagtacac ggtgatagat
      841 atgaatttaa agaaatctga tttatcaact gaccgcctta cacgtgctga agctgctgcc
      901 taccttggcg ttaacaatca aacgctggcg aactgggcct gtactggcaa ggtaaaaatc
      961 cctttccata agctggggcg caaagtgctg tatatgcgca ctgatctcga tgcttatctt
     1021 gcatccactc gcagaacgca gacagtgtaa gggggagcga tgcacataac aaaaagcgcc
     1081 tcgttgccgg agcgcccttg cgaacaatta acctactgcg caaaaaatga atctgtgcag
     1141 ggggattata tcaaccatgt agcgaagcgc cactattgcc ggataacagg caaagaaaag
     1201 gccacccgct acggtggccc ctcgacacaa gctacacgtt atccccaacg catgagcatt
     1261 gccaacaatg ccacatttgc agctggtggg caatgtgatc agtgtgctgg cttgctggtg
     1321 ggctattcct gctctttagc atcaccatac tgcgatgttt tccaagtatg cgat
//
LOCUS       DAHBHC010000293         1346 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHBHC010000293 DAHBHC010000000
VERSION     DAHBHC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.298"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(132..>1346)
                     /locus_tag="M2A35_005473"
     CDS             complement(132..>1346)
                     /locus_tag="M2A35_005473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201597.1"
                     /translation="LFFSLAAGGTHASQIQVSGQSVNFASPGAITLTNDWLNGKKYPD
                     NASVYTWNIQESSSNGGCGNLCTTLMQNIRSGQARGDAVFTPRGEQSVVTKVGTVKIK
                     TTFNNFKFYGLFTVNGAQKGNNYFPTDSTYTDFNSGSYTYSLNSELLPQCLLLGCTFS
                     WKTGISTGKVNLQIEIPDKLSTRTFTIPETPVATFTTTLYSQVVDSGYVGDTYVMSSS
                     VNIPEITITIPERCYTKISGDIAGNTVTFSSDIDASTVTAEGAKSLDAKSIQLTAGCN
                     TKYISKSVFASVKIMPTNGVEDGYKFKLTPRQSATPGADRYLSVVAKHLSQGAAGATT
                     CGNDANTFESGKFYNVGRVAFPESNNALKDPYPVTFNLCAFNTANSENLLIPGAYTGG
                     VRLVTRFYTADQ"
BASE COUNT          371 a          297 c          279 g          399 t
ORIGIN      
        1 tgcgttaaat tttccggttt caggcgtggt gacggtatta ttgccatata aaaaatacca
       61 caatgaaatt acaaccagca tgatggtatc atgctggctc atcttatcgt tttcagacag
      121 gcattatgca gttactgatc tgcagtgtaa aagcgcgtca ccagcctgac accaccggtg
      181 tatgctccgg gaataagcag gttttctgaa ttagctgtat tgaacgcaca cagattaaat
      241 gtcacaggat aaggatcttt aagtgcgtta ttcgactcag gaaatgcaac acgcccgaca
      301 ttgtaaaact taccactctc aaaagtgttt gcatcattac cacaggttgt ggcccccgcc
      361 gctccctgtg ataaatgttt ggccacaaca ctcagataac ggtctgctcc gggggtggca
      421 gactgcctgg gcgttaattt aaatttataa ccatcctcga caccatttgt cggcataatc
      481 ttaactgagg caaaaactga tttactgata tattttgtat tacacccagc tgtcagttga
      541 attgacttag catccagtga tttagcaccc tctgccgtga cggtcgaggc atcaatatca
      601 ctgctgaatg taacagtgtt tcccgcgata tctcccgata tttttgtgta acaacgttca
      661 ggaatagtta tggttatttc cgggatattg acagagctgc tcattacata ggtatcaccg
      721 acataaccac tatccacgac ctgagaatat agtgttgttg taaatgttgc aacaggagtc
      781 tcaggtattg taaaggtccg ggtactcagt ttatcgggaa tttcaatctg gagattcact
      841 tttcctgtac tgatgcctgt tttccatgag aatgtacacc ccagaagcag acattgcggc
      901 aggagctcag aattcaagct gtatgtataa gagccactat taaagtcagt gtaggtgcta
      961 tctgtaggga aatagttatt acctttctgt gcgccattta cagtaaaaag gccataaaat
     1021 ttaaaattat taaatgttgt ttttatcttt acggtaccaa ctttcgtgac aactgattgc
     1081 tcacctcttg gcgtaaatac agcatctccc cgagcctgac ccgatctgat attctgcata
     1141 agagttgtgc acaggttgcc acaccctccg tttgaagaac tttcctgaat attccatgtg
     1201 tacacactgg cgttatccgg atatttcttt ccgtttaacc agtcattagt caatgttatt
     1261 gcgccagggg atgcaaaatt tacagactgt ccactcactt gtatctgtga agcatgagta
     1321 ccgccagctg caaggctgaa aaataa
//
LOCUS       DAHBHC010000294         1310 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHBHC010000294 DAHBHC010000000
VERSION     DAHBHC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.299"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..606
                     /locus_tag="M2A35_005474"
     CDS             <1..606
                     /locus_tag="M2A35_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCB7201598.1"
                     /translation="DADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVYQNQPETEQ
                     NDPNTQQNEPETKQPEPVVQQQETEKVCTACGKAGGGNCPDCGAVMGDATYQKTFNEE
                     NQNEYQEKGLEEMEGAEHSHKENAGSDPHRDCSDETGEAAASSLEKLDWKRQVVIAAV
                     YGLCANPAGIASAPLIPGIAMMIANKLENFGVTDDEHIPDF"
     gene            584..880
                     /locus_tag="M2A35_005475"
     CDS             584..880
                     /locus_tag="M2A35_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCB7201599.1"
                     /translation="MSTYPIFDRIEEQAWSRHYQQIVREEKETALADDLEKGLPQHLF
                     ESLCIDHLQRHGASKQAISRAFDDDVEFQERVAEHIRYMVETIARHQVDIDSEV"
     gene            886..>1310
                     /gene="bet"
                     /locus_tag="M2A35_005476"
     CDS             886..>1310
                     /gene="bet"
                     /locus_tag="M2A35_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HCB7201600.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELIATLRQTAFKGNASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGKEIIGP"
BASE COUNT          383 a          307 c          355 g          265 t
ORIGIN      
        1 gacgccgata agttgcttgc tgcctctcgc ggagaatttg ttgcagggat tagcgacccg
       61 aatgatccga aatgggttaa ggggatccag acccgcgact ccgtgtacca gaatcagcca
      121 gaaacggaac agaacgaccc aaatacgcaa caaaacgagc cagaaacgaa acagcctgag
      181 ccagtagtgc aacaacagga aacggagaaa gtttgcaccg cctgcggtaa ggctggcggc
      241 ggcaactgtc ctgactgtgg cgcggtgatg ggcgacgcaa cgtatcagaa aacctttaat
      301 gaagaaaatc agaatgaata tcaggaaaaa ggtctggagg aaatggaagg cgctgaacat
      361 tcacacaagg agaatgctgg cagcgatccg catcgcgatt gcagtgatga aactggcgaa
      421 gcagcagctt catcattaga aaaactcgac tggaaaagac aagtggtgat tgcggcggtt
      481 tacggtttat gtgcgaatcc tgcaggtata gcctcagcgc cattaattcc gggtattgca
      541 atgatgattg caaacaaact tgaaaatttt ggggtaacgg atgatgagca catacccgat
      601 ttttgatcgc attgaagagc aggcatggtc acgccactac cagcaaatcg ttcgcgaaga
      661 aaaagaaacg gcgctggcgg acgacctgga aaaaggtctg ccccagcacc tgtttgaatc
      721 gctctgcatc gaccatttgc aacgccacgg tgccagcaaa caggcaatca gtcgcgcatt
      781 tgatgacgat gttgaatttc aggaacgcgt ggcggagcac atccggtaca tggttgaaac
      841 cattgctcgt caccaggttg atattgattc agaggtataa aaccaatgag tacagcactt
      901 gcaacactgg ccgggaagct ggctgaacgc gtcggcatgg attctgtcga cccgcaggaa
      961 ctgatcgcca ctcttcgcca gacggcattt aaaggtaatg ccagcgatgc gcaattcatc
     1021 gcactgctga tcgtcgccaa ccagtacgga cttaatccgt ggacgaaaga gatttacgcc
     1081 ttccctgata agcagaacgg cattgttcct gtggtgggcg ttgatggctg gtcccgcatc
     1141 ataaatgaaa accagcagtt tgatggcatg gattttgagc aggacaatga atcatgtaca
     1201 tgtcggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgagtg gatggatgaa
     1261 tgccgtcgcg aaccattcaa aacccgagaa ggcaaagaaa tcatcggacc
//
LOCUS       DAHBHC010000295         1306 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHBHC010000295 DAHBHC010000000
VERSION     DAHBHC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.300"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            414..632
                     /locus_tag="M2A35_005477"
     CDS             414..632
                     /locus_tag="M2A35_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCB7201601.1"
                     /translation="MAQKRLIAELEKQTTGITVVTKEGFAHELLAVEYSGWISPAFRL
                     QVKQTFIDYRTGKRNGNRYGCWWAASSS"
BASE COUNT          337 a          319 c          326 g          324 t
ORIGIN      
        1 ttaagccaga cacagcaaag gatgaaacaa aaaattaaca atgggacatt gggacgcaaa
       61 attgaaaatt tatagctagc gaaacaccgc gctcgcgcaa tgcccgtgca ataactcgct
      121 caggaaggac ccgttacccc cctggggggt taatgagatg actatactca ccatgccaca
      181 gtttgcccgg tgtcttcctc tggtgcggat tcacgtcatt gcgctggtgg cgcttctgtt
      241 attgctccac ggtaacagga gttcaggcct gttaagctgg agcacatcaa acaactggaa
      301 gcaaaacggc gatgcctctg ctcccgtagt tgcgccactt ttctaccaac gggaggggat
      361 ctatcgtccg aaatccggac gaagggacgc atcaacttat tggctagcgc ttgatggcac
      421 aaaaaaggct gattgcagaa ctggaaaaac aaactaccgg gatcacggta gttacgaaag
      481 aaggatttgc tcacgaacta ctggctgttg agtattctgg atggatttct ccagcgtttc
      541 ggttgcaggt gaagcagact tttatcgatt atcgtactgg aaaacgtaac ggaaatcgtt
      601 atggttgctg gtgggctgcg tcatcatcat gatgataatg ctgtccagtc agccggaacg
      661 gttgaacagg gtggctatgg gtcactctgt tgtgcgttat tagcgtgttc ccggtagggt
      721 gtgactatct ttcagatagt cggttgctcc ggcctctgtc gttggctgtt tccgaaaata
      781 tcagcggctc aggccaaagc ccgaaaaagg gctttggaaa aaattaactg aataccgtcg
      841 ctttaacctg cgataatcgt tctgccccgg tgcgtgtcct ctccgtggtc attttgacca
      901 tgcagctacc accctgccac acaaaaggga tggtcattat caccatcccc tttaaagcct
      961 cttgaaagag gcctaccact accagctata tctctcaaat ccaccagcgc aacgctggac
     1021 aaaaaaacca ttacgatgac tcaggacgca aaaccacgtt taacgttgtt ttgcactgga
     1081 ccagaatact gatcgtcttt gtctcgatgt ccggctgcgt ggtctcctgt ttgcctgctg
     1141 gtgggggcta taagtcctgc gcaaattttg gctccgtaat gaaaaaactg acaaccgaca
     1201 gatgccagga gcataacaaa atctgaaact caccagcgcc atcttgttac taggcgtctg
     1261 aaagcataag gttgtgggcg cgaagtgctt cccgtgaagt gctttt
//
LOCUS       DAHBHC010000296         1300 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHBHC010000296 DAHBHC010000000
VERSION     DAHBHC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.301"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            27..554
                     /locus_tag="M2A35_005478"
     CDS             27..554
                     /locus_tag="M2A35_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201602.1"
                     /translation="MKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPT
                     PDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQ
                     DDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGE
                     ILAKMKQRTSRGIRK"
     gene            551..1120
                     /locus_tag="M2A35_005479"
     CDS             551..1120
                     /locus_tag="M2A35_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201603.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIEIQHELIRSEEK"
BASE COUNT          438 a          231 c          271 g          360 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgc
       61 tgaatccggt tcagtatata gattcaatgg gactggcatc aaaatatgga cacttaaata
      121 atggcggata tggagcgaga cccaacaaac cgcctacgcc cgatccaagt aaattgccgg
      181 acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt gcgcctaatg
      241 ttttcaaaac attcttcaga gcattaagcc cttacgacta cacactgtat tgcaggaagt
      301 gggtaaaacc aaatctgact tgtacgccac aggatgattc ccagtatcca gggatggata
      361 caaagacagc aagtgattac ctgccacaga caaattggcc aacaactcaa ttaccaccag
      421 gatatacttg tgcagaaccc tatttattcc cagacattaa taaacccgat gggccagcaa
      481 cagcagggat agatgatttg ggtgaaattt tagctaagat gaaacagaga acatcgagag
      541 gaataagaaa atgaaaagag ttttgttctt tttgctgatg atatttgtta gttttggtgt
      601 tatagctgat tgcgaaatac aagctaaaga tcatgattgt tttactattt tcgctaaggg
      661 tactattttt tccgcgtttc ctgtcttaaa taataaagcc atgtggagat ggtatcagaa
      721 tgaggacatc ggtgagtatt attggcaaac agaactgggt acatgtaaaa ataataaatt
      781 cacaccaagc ggggcgagac tcttaattcg cgtcgggtca ctacgcctta atgaaaatca
      841 tgccatcaaa ggaacgttgc aggagttgat aaatacagca gaaaaaacgg cgtttctcgg
      901 cgatcggttt agaagttata taagagcggg tatctatcaa aaaaagagca gtgatccagt
      961 tcagttgttg gcagtccttg ataattcaat catggtaaaa tattttaaag atgagaaacc
     1021 aacttatgct cgaatgacag cacatctccc gaacaaaaat gagtcttacg aatgtttaat
     1081 agagatacag catgaactaa ttcgtagtga agagaaataa aatctcttcc tgagaaaaca
     1141 aattaatcat tgtgaaaaat tatataactc atggaatagg tgtttatttt gcgttccatt
     1201 tgcagggaaa gatcacgtaa cgctactttt ttgtactaaa taattcgaat tttaagttca
     1261 acaattgaga tactacttat tgtctaaagc tgtttttcat
//
LOCUS       DAHBHC010000297         1296 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHBHC010000297 DAHBHC010000000
VERSION     DAHBHC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.302"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..197)
                     /locus_tag="M2A35_005480"
     CDS             complement(<1..197)
                     /locus_tag="M2A35_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS regulator Mpc"
                     /protein_id="HCB7201604.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALA"
     gene            381..683
                     /locus_tag="M2A35_005481"
     CDS             381..683
                     /locus_tag="M2A35_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HCB7201605.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTPENGGSRNIRI
                     VAGIALATTALAALGTGIAMACTTNASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            767..1144
                     /gene="sctI"
                     /locus_tag="M2A35_005482"
     CDS             767..1144
                     /gene="sctI"
                     /locus_tag="M2A35_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HCB7201606.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            1146..>1296
                     /locus_tag="M2A35_005483"
     CDS             1146..>1296
                     /locus_tag="M2A35_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HCB7201607.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKE"
BASE COUNT          462 a          227 c          233 g          374 t
ORIGIN      
        1 gcaagggcat tgaaaaaaga aaaaaacgca ttttcgttat actctaaggc aaacgtaata
       61 aacacattac tatcaattat ttcaataaaa aaagaaaaac cattaaaatc aatttccaca
      121 cgattatcaa acaaataaaa atcatttcct aacaatctta aaaactcttc tacgtttctt
      181 ttaattaaaa gattcatatg caggctctga agtaagtata tgtgtggata ttattgcttg
      241 aaaaagcgta ctggataata tatacagtat atgtacttta tgtacattgc aactatttaa
      301 ataatataat agttgcccat gggataattt ggttattgat aaccagacaa atgtctaaat
      361 tagacaatgg agaaaagtta atggatgcag caaatttaag tccttctggc ggagtattac
      421 cactggcagc cacaatcaat ggaaatgttt cggtggatga aaatacagga gtaatgacac
      481 cagaaaatgg gggaagtcgt aatatcagaa ttgtagccgg aattgcactc gctactacag
      541 ctctggctgc tttaggtact ggtatcgcaa tggcatgcac aacaaatgca agctcccaag
      601 aattcttagg tttaggtatt gctaccggtg tattaggtgg tgttactgcg cttggcggcg
      661 gattagcgat gaaatatgcc taatcatcgc atcgcataaa taaaatagag aggtcatgga
      721 tgcattatgc cattacctct ctcatgaaaa gagactaacc gtaaatatga acaatattaa
      781 tcaatcagaa aatattaata ttcattttaa taaagtatcc caaacaaatt tcgttgatga
      841 ccataccccg ttagcaccaa cgccctctgc ggctggagcg gctcagtttc ttgatcaatt
      901 acttcctaaa acagctggtg tatcttctcc agagcaagtt ttaattgaag aaattaaaaa
      961 gagacatctt gcaacaatga acaccgatct cagtttcgat gccctatctg caggtggact
     1021 ctcgccagaa gatgtgctca cattacaaaa gaatgtgctc aacgcaaacg tcaatgttga
     1081 tgtagtatcc aagttagcaa gtcttctttc aacatcggtt acaaaactgg tttcaatgca
     1141 ataaaatgag aaaatacatt aaaaacacct ttttatttgt ttttatttgt ttgacagttg
     1201 catgtaaaga gcaattgtat acaggattaa cggaaaaaga agccaatcag atgcaagcat
     1261 tgttgttatc caatgatgtc aatgtatcta aagaaa
//
LOCUS       DAHBHC010000298         1275 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHBHC010000298 DAHBHC010000000
VERSION     DAHBHC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.303"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>1275
                     /gene="rhsD"
                     /locus_tag="M2A35_005484"
     CDS             <1..>1275
                     /gene="rhsD"
                     /locus_tag="M2A35_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HCB7201608.1"
                     /translation="FGRTLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEA
                     RTSSLSSSDSSRPLSASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVR
                     YTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDAGRVVEQL
                     NPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAG
                     RLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRR
                     EYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDC
                     SGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLT
                     AVITPDGNRSETQYDAWGKAVSTTQGGLTRSM"
BASE COUNT          266 a          377 c          446 g          186 t
ORIGIN      
        1 cttcgggcgg acgctgacgt accggcgtga ggccgccggt gacctggccg gggaaatcac
       61 cggcgtgacg gacggtgccg ggcgggagtt ccgtctggtg ctgaccacgc aggcgcagcg
      121 tgcggaagag gcccgcacct cttcgctatc ttcttctgac agttcccgcc ctctctcagc
      181 ctcagcgttc cccgacacac tgcccggtac cgaatacggc cccgacaggg gtatccgcct
      241 ttcggcggtg tggctgatgc acgacccggc atacccggag agcctgcccg ctgcgccact
      301 ggtgcggtac acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac
      361 gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc cggatggtgg ggcaccgtta
      421 tgcaggcagg ccggagatgc gctaccgcta cgacgatgcg gggcgggtgg tggagcaact
      481 taacccggca ggcctgagtt accgctacca gtatgagcag gaccgcatca ccgtcaccga
      541 cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga agcgggtggt
      601 gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg caggaaggct
      661 cacggcgcag acggacgcgg cgggacggcg gacagagtac ggtctgaatg tggtgtccgg
      721 cgatatcacg gacatcacca caccggacgg gcgggagacg aaattttact ataacgacgg
      781 gaaccagctg acggcggtgg tgtacccgga cgggctggag agccgccggg aatatgatga
      841 accgggcagg ctggtatcgg agacatcgcg cagcggggag acagtacgct accgctacga
      901 tgacgcgcac agtgagttac cggcgacgac aacggatgcg acgggcagca cccggcagat
      961 gacgtggagc cgctacgggc agttgctggc gttcactgac tgctcgggct accagacccg
     1021 ctatgaatac gaccgcttcg gccagatgac ggcggttcac cgcgaggaag gtatcagcct
     1081 ttaccgccac tatgacaacc gtggccggtt aacctcggtg aaagacgcac agggccgtga
     1141 aacgcagtat gagtacaacg ccgcaggcga cctgactgcc gttatcaccc cggacggcaa
     1201 ccggagcgag acacagtacg atgcgtgggg aaaggcggtc agcaccacgc agggcgggct
     1261 gacgcgcagt atgga
//
LOCUS       DAHBHC010000299         1266 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHBHC010000299 DAHBHC010000000
VERSION     DAHBHC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.304"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            267..482
                     /locus_tag="M2A35_005485"
     CDS             267..482
                     /locus_tag="M2A35_005485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201609.1"
                     /translation="MFDIVKSPKVTIQTKRIFMSEKNKPQGENKPQQPVAPKPTPTQS
                     TADFATRRVFVGDSADSVIEHIKKQPR"
     gene            complement(451..1020)
                     /locus_tag="M2A35_005486"
     CDS             complement(451..1020)
                     /locus_tag="M2A35_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201610.1"
                     /translation="MRELPKVYFLGVDDELVDYLEKQGEETIREIHLSNKTNVENGYK
                     LLNIQIVGIGSSFLLLTQKTNFDFLTAGITTFTLLWTWCAIYLVCTGLSVKVRGLINA
                     PPDHLYHEKYKDMEPSSFKIFADAGYLGPDKLLPLIRRYRLVDLSDTARELLLENEKI
                     RTSLDKARMYTILAPVAAMFISAVFLYVQ"
BASE COUNT          385 a          295 c          254 g          332 t
ORIGIN      
        1 caattgacat aaccactgcg cccaacggcg tatctccacg ccaccaactt tgtaagagtt
       61 ccagtaagtc aggccaggaa tgaggggcat tgtgcatttt cataagcctc acctccgaga
      121 gttcggatgg tgctaagtgt aagattcagg ctctcaggct tgctaacaag aagtcgagga
      181 tgtttccgga gcctaacaac gaaaaagccc cgggacatgc cggggccaga tggagtgcca
      241 gattaagctt ctggcggtat atactcgtgt ttgatatcgt taaatcgcca aaagtaacaa
      301 ttcaaacaaa gaggattttt atgtctgaaa aaaacaagcc acaaggtgaa aataaacctc
      361 agcaacccgt ggcaccaaaa ccaactccaa cacaaagtac tgcagacttt gctacacgtc
      421 gtgtttttgt tggagattct gccgactcag tcattgaaca tataaaaaaa cagccgagat
      481 aaacatcgcc gctaccggag caaggatagt atacatcctt gctttatcga gactcgtgcg
      541 gattttctca ttttccaaca gtaactctct tgctgtatca ctcaagtcaa caaggcgata
      601 ccttcgtata agcggcaata acttatcagg tcctaaatat cctgcatcag cgaatatttt
      661 aaagctcgag ggctccatat ccttatattt ttcatgatat agatgatcag gaggggcatt
      721 gatcaggccc ctaaccttca cagataaacc agtacatacc aagtaaatgg cgcaccatgt
      781 ccatagtaat gtaaatgtgg taattccggc ggtgagaaaa tcgaaattag ttttctgtgt
      841 cagcaataaa aaagatgagc caattccaac aatctgaatg ttcagaagtt tgtatccatt
      901 ctcaacattg gttttgttag aaagatgaat ctctcgtatc gtctcttccc cttgtttttc
      961 aagataatcg acgagctcat catctactcc taaaaaataa actttaggta gttctctcat
     1021 ctcacctcca catcctgtac tgaacacaat tttaccagaa tgtctcgatt ccaggtattc
     1081 cgccaggaat cgcgctccag aaatgaaaca tcaggttcgc cagtaccaga aacaacaaaa
     1141 cccgctcaat ggcgggttct ggtaaagttc atgcgcttgg ttcgcctcgc gatacagctt
     1201 tgcgaagcgt accgggattg aagcagttta tggctaaaat tgcaagaact tttttaaagc
     1261 tgcatc
//
LOCUS       DAHBHC010000300         1256 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHBHC010000300 DAHBHC010000000
VERSION     DAHBHC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.305"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1251)
                     /gene="istA"
                     /locus_tag="M2A35_005487"
     CDS             complement(<1..1251)
                     /gene="istA"
                     /locus_tag="M2A35_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCB7201611.1"
                     /translation="MRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYANRAAQLGI
                     KQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEEYCERN
                     PGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEIRTAQ
                     VIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRACKY
                     DPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSLAS
                     LNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHADY
                     HVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPE
                     AHRQHATWTPERLLEWAGHIGSETH"
BASE COUNT          301 a          313 c          305 g          337 t
ORIGIN      
        1 atgagtttca ctgcctatgt gccccgccca ttccagaaga cgctctggcg tccaggtggc
       61 atgctgtcga tgagcttcag gcatgtgctc gttgcgggta ctgtagccat aagtacgctt
      121 gcgcgggtgc acagcaacct cctgcccctg attgaagagt cttaccagtt ctccggagat
      181 ccatgcttcc agttgctggc ctaacagcga acatggaacc gagtagtaat gtttgtcgat
      241 ttccacgtgg taatcggcat gaactctgac tttcttcacc agggtgtaac tgtaactggc
      301 ttcaggaaga ggcttcagtg cgggtttatc aagctggatg aagagttctg cacgtgaata
      361 acccaacttc tgcattattt tgttattcag tctttccagc aactcccgaa tgcgctgatt
      421 aagcgatgca aggctgtaga agatctcatg cctgattcgg gccatgatcc agcgttcaac
      481 aacctgaacg ccaacttcag ctttggcttt atctttcggt ttacgtggcc gcgcaggcaa
      541 aactgcgaca ttataatgct caagcatctg ctggtaggta gggttaacgt caggatcata
      601 cttacatgcc ctggatgtgg cgcttttcag attgtccgga acaacaagtt caggaacgcc
      661 acccaaccac tggaagcagc gaacatgact catcacccag tcttcaagct gctgagacca
      721 ggtggcctct gcccatgtgt aacttgatgc cccgagaaca gctacgatga cctgagcagt
      781 tcttatttct ccggtctcag ggtcggtaac gccaacggta ggtccacagt aatcaacgaa
      841 aagtttttcg ccagctttat gtacctgacg cattgatggt gaagtggttt tgagccattc
      901 acggtacatc cggcagtaat ggttatagct gtaaaaaccg cctggattac gctcacagta
      961 ttcttcccag agtagctgca gcgtcacgca tttattacgc agttcccggt gtactgtagc
     1021 ccagtcaggc agagagtgct tcttcatctt aacctgggtc tgaaggaacg catgttttag
     1081 ttttgtatca tcccatcctg taggtaaggg ccactgcttt atgccaagtt gagccgcccg
     1141 attagcatat ctggatacaa cggaagggga gattgcaaga ctacgaccaa tttgtcgatg
     1201 gctgagtcca acaccgtatt taagcctaag aatttcttta agttttctca tagaaa
//
LOCUS       DAHBHC010000301         1251 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHBHC010000301 DAHBHC010000000
VERSION     DAHBHC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.306"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            183..1163
                     /locus_tag="M2A35_005488"
     CDS             183..1163
                     /locus_tag="M2A35_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HCB7201612.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          306 a          309 c          371 g          265 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg
      601 cgctgacaga tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga
      661 gaccgagcat tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc
      721 agataaaaga cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa
      781 gcatcccggg tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg
      841 accgcttcgc ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg
      901 aatcaggtag cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc
      961 gcagggcgtt gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt
     1021 tccgcgaccg tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc
     1081 gcaagctggc acaggtggcg tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac
     1141 ggcataatcc ggtagcggcg taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg
     1201 cgctgtgtct ggcatgcctg caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAHBHC010000302         1250 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHBHC010000302 DAHBHC010000000
VERSION     DAHBHC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.307"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..157
                     /locus_tag="M2A35_005489"
     CDS             <1..157
                     /locus_tag="M2A35_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCB7201613.1"
                     /translation="IRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIE
                     RGVALTD"
     gene            164..1060
                     /locus_tag="M2A35_005490"
                     /pseudo
     CDS             164..1060
                     /locus_tag="M2A35_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            1104..1250
                     /gene="tnpB"
                     /locus_tag="M2A35_005491"
                     /pseudo
     CDS             1104..1250
                     /gene="tnpB"
                     /locus_tag="M2A35_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          359 a          295 c          317 g          279 t
ORIGIN      
        1 aattcggcag tactttccta aaaagacatg tcttgcccaa tatactcaac atgaactaga
       61 tctggttgct gctcagctaa acaacagacc gagaaagaca ctgaagttca aaacaccgaa
      121 agagataatt gaaaggggtg ttgcattgac agattgaatc tacaacagcg tattttgcac
      181 aggagtcgct gaaaaatacg cgttaatcga acaatggcga caacaatttc ccattgaagc
      241 gatgtgtcag gtatttggtg tatccaagag cggttattac aactgggtac agcatgaacc
      301 ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg cacatatccg
      361 cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga atggcatcat
      421 cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct gtaagcagaa
      481 acgcaagttc agagcgacta cgaactcgaa ccacaatctg ccagttgcgc caaatctgct
      541 gaaccagacg ttcgctccta cagcacgaaa tcaggtctgg gtggcggacc tgacgtatgt
      601 tgccacacag gagggatggt tgtacctcgc tggcatcaaa gatgtttata cgtgcgaaat
      661 tgtcggctac gccatgggag agcgcatgac aaaagagctg acaggtaaag ctctgtttat
      721 ggcgctcagg agccagcgcc cacctgccgg gctaatccac cactctgatc gaggttcaca
      781 gtactgcgca tacgattacc gggtcataca ggagcagttt ggtctgaaaa catcaatgtc
      841 gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa
      901 tgagagcctg agccactatc gttttaataa ccgggatgaa gccatctcag taatacggga
      961 atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga atatctcccc
     1021 ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaaca aatggtagta
     1081 tccgctattg ccagtacacc tcaaacccct tctccggtca cctgtttatc ttccgtggcc
     1141 gacggggaga cacgattaaa atcctgtggg ctgatgctga tggtctgtgc ctgttcacca
     1201 aacgccttga ggaaggtcag tttatctggc ctgcggtgcg tgacggtaag
//
LOCUS       DAHBHC010000303         1247 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHBHC010000303 DAHBHC010000000
VERSION     DAHBHC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.308"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..1247
                     /locus_tag="M2A35_005492"
     rRNA            <1..1247
                     /locus_tag="M2A35_005492"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          311 a          291 c          391 g          254 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga
      721 gatggattgg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca cctcctt
//
LOCUS       DAHBHC010000304         1246 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHBHC010000304 DAHBHC010000000
VERSION     DAHBHC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.309"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            36..473
                     /locus_tag="M2A35_005493"
     CDS             36..473
                     /locus_tag="M2A35_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCB7201614.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNLYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVFATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            623..1240
                     /locus_tag="M2A35_005494"
     CDS             623..1240
                     /locus_tag="M2A35_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCB7201615.1"
                     /translation="MLASLGGLVSYAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
BASE COUNT          350 a          275 c          317 g          304 t
ORIGIN      
        1 gagattgagc gtgaagtctg tttgtggggg caacaatgag cagggtaacc gttattatct
       61 ccgctctggt tatctgcatt atcgcctgcc tgtcatgggc tgttaatctt taccgtgata
      121 acgccatcgc ctacaaagag cagcgcgata aagccacatc catcatcgct gatatgcaga
      181 agcggcaacg tgatgtagca gaacttgacg ccagatacac aaaggagctt gctgatgcta
      241 atgcgactat cgaaagtctc cgtgctgatg tttctgctgg gcgtaagcgc ctgcaagtct
      301 tcgccacctg tgcaaagtca acgaccggag ccagcagcat gggcgatgga gaaagcccag
      361 gacttacagc agatgctgaa ctcaattatt accgtctccg aggtggaatc gacaagataa
      421 ccgcgcaggt taactacctg caggaataca tcaggacgca gtgcttaaaa taattttaat
      481 ttcactgaaa tttaacaagt gactttcagg aaaatgcctc gcagatgcgg ggcatttttg
      541 taccggtatt tcaccgcgca ccgcagcgca caataaacac cgaacctgac cctttggaat
      601 gggcctttga ggataccagt tagtgctggc gagcctcggt gggctggttt cctatgcggc
      661 aaaggttcat ttcaaagaag caggcaacgc catgaatgaa ttaattgcga atcatgactt
      721 cgactttcgc cagttagtta ccgcagcaga aggtcaaccg gtaactgaca ccttccagat
      781 tgccagggca tttggtaaac gccatcagca tgtgattagg gctattaaat gtttgagatg
      841 ttctgaggaa ttctcgacaa cccatttttg ggccgtcgag aaaatcaatg acttaggtat
      901 ttttgacaag aaacagattt actaccgcat ggactttagt ggcttcgtta tgctggttat
      961 gggatttaac ggggcaaaag ccgatgctgt taaagaagcc tatatcaatg cgtttaactg
     1021 gatgtcagca gaactccgta agtacagcga aagttatgaa gcagaacgta acgccgtaat
     1081 gctggagtac atgaaagaga aggatgtcgc cagcatgtca ggccgtctgc tcaatcgctg
     1141 ggggagaacg aaaaaacctc aattgcttgc aaagctggaa cgtctggaga gacagggaca
     1201 gtttttatta ccgggattcg ataaaggtat tcaagcctga cacatt
//
LOCUS       DAHBHC010000305         1241 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHBHC010000305 DAHBHC010000000
VERSION     DAHBHC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.310"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(195..710)
                     /locus_tag="M2A35_005495"
                     /pseudo
     CDS             complement(195..710)
                     /locus_tag="M2A35_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            848..1036
                     /locus_tag="M2A35_005496"
     CDS             848..1036
                     /locus_tag="M2A35_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003033726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201616.1"
                     /translation="MPTKHINDVLWRKVEKETVKVVTTLAIPVKDTKVLEWIIAKGLE
                     SITEDDYRKFAKTDATKK"
BASE COUNT          424 a          260 c          286 g          271 t
ORIGIN      
        1 cctttaccgg aatggcaaaa gatacagatc aacacacctg tgaagttcca ataacattga
       61 tggagatcac tgaataacaa aatgagtcaa aagtgatctc catcgatgtt attcagcgat
      121 ctgttcaaat gttattaccc gatctcgatg gaagttattg agtgatctcc tttcatgaaa
      181 atacgcagct ctggtacatc actataaatg acccaagaaa caaggcagtc acgaacaacc
      241 gaatcagcca cctcagcaag ctgctcatca gtgcagaaaa gcgagaattc ataagggtcc
      301 ccgtcctgat taaagaaagc gaaacggtgc gcagactcaa gcgccatcag cgaatttttc
      361 acaccgagct gataaaccag acggcagaaa gcgtgcgcca cggtgaagga aaacgaacca
      421 aactgtgata ccagtccggc agccttgccc ctgtcagcga gagataaatc cgtgcagtta
      481 attccgtaaa tcacgcggtc aacctgcgtg ttacgtggca gggcaagctg tgaagagagc
      541 accccgacct catcacgata ctggcgggaa agcgtggcaa ggttgcgagt ggcaacatcg
      601 cgctcagcct gaaaatgctc acgttcgggc agtgtcagac aaggacgacc gagagcaacg
      661 gatgccgcgt cgatggcgtt ataggctgtg aggatggctg attcagtcac gcaaagcccc
      721 caagagaaca aaacttagaa tctaaatatt tatttattgg tgtgtttata atttcttagt
      781 ttctaagtgt caaggattct aagttttgag atgtatgatg agctatggaa aaacagaggg
      841 gacaaatatg ccaacaaagc acataaacga tgttttatgg agaaaggtag agaaagaaac
      901 tgtaaaggtg gtaacaacac tggcaatacc agtaaaagat accaaagttt tagaatggat
      961 aatcgcaaaa ggactggagt ctataacaga agatgactac aggaaatttg caaagacgga
     1021 cgcaacaaaa aaataacatc atagttacct atcgatacga agattgctaa gaagcaatat
     1081 gcagacaacc agtgacttaa ttcttgacct gctgataaag cattttgttg tggatagcat
     1141 tacaggcatg acgctgttcg gcctgataac gcttgttctt ccgttggtga tgaccataca
     1201 cgacaaaata aacagagaca gaaaaaacgc ttaaaaacca a
//
LOCUS       DAHBHC010000306         1229 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHBHC010000306 DAHBHC010000000
VERSION     DAHBHC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.311"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..464
                     /locus_tag="M2A35_005497"
     CDS             <1..464
                     /locus_tag="M2A35_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCB7201617.1"
                     /translation="RWNTTRAGAFNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDA
                     NPAPGKSLKEIVISAPDGAVFRYDADAGALSASGMKTATLQASVSVKLDTPVVECTNL
                     LRTATLDVTKGGKMSGNITHSGGNFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            482..811
                     /locus_tag="M2A35_005498"
     CDS             482..811
                     /locus_tag="M2A35_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCB7201618.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            815..>1229
                     /locus_tag="M2A35_005499"
     CDS             815..>1229
                     /locus_tag="M2A35_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCB7201619.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSG"
BASE COUNT          301 a          354 c          368 g          206 t
ORIGIN      
        1 tgcgctggaa caccacgcgc gcgggagcct tcaatgtgtg gctgccgcca tcaccaggcg
       61 aacaggtggt aattgcctgc attggcggca acccggaaac cgccatgata attggcagcc
      121 tgtggagtga tgccaatccg gcacccggca aaagcctgaa agaaatcgtg atcagcgcgc
      181 cggacggcgc ggtgttccgc tacgacgcgg acgcaggcgc actgagcgcc agcggcatga
      241 aaacggccac tttacaggca tccgtcagcg tgaaactgga tacgcccgtc gtggaatgca
      301 caaaccttct gagaacggcg acgcttgacg tcacaaaagg aggaaagatg agcggcaata
      361 tcacgcacag cggcggcaac ttcacctcaa acggcattac cgtgcatacg cataaacacg
      421 gtggcgtgaa aggcggcagc gattcgacag gaggcccgca gtgacaaccc gctacacagg
      481 aatgaatccg gacgggacgg gaaacctgaa cgatatggaa cacctgaaac agtcagtcag
      541 ggacatcctg accaccccgc tggcaagccg ggttatgcga cgggaatatg gcagccttgt
      601 gcctgatttg attgacgaac ccatgaataa caccacgcgt ctgcaatgca tgagtgctgc
      661 cgtgattgca ctgacacgat gggaaccccg cattgccctg gatgccatcg atgttgtctg
      721 gaaggcagga ggccgcgccg gggtgacgct gtcgggcacc gtcatgcaga ccatgcagaa
      781 tgttgaatta accatcacgc tgagagagta aatcatgcct gccgttgacc tttcccagtt
      841 accggaaccc gccatcatcg cggagcctga ttttgaggca attctggctg acacaaaggc
      901 catgatgatt gcagcttatc ccgccgaaca gcgtgaagcc gtctccgccg cgctggagct
      961 ggaatcggaa cccctgaacg ttatcgccca gacaacagcg tttcgtgaaa tgctgttacg
     1021 ccagcgggtc aatgagggtg cacgcgcctg catgttaagc cacagcgccg ggacagacct
     1081 ggacaacctc gcgggcaata tgaacacaaa gcgcctggtt atcactccgg caacggatac
     1141 caccgacgcg gtgatggaga gcgacacctc gctgagactg cgggcgcagc gggcgtatga
     1201 cggcctgagt gttgcaggcc cgtcaggcg
//
LOCUS       DAHBHC010000307         1224 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHBHC010000307 DAHBHC010000000
VERSION     DAHBHC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.312"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>1224
                     /locus_tag="M2A35_005500"
     CDS             <1..>1224
                     /locus_tag="M2A35_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCB7201620.1"
                     /translation="PSAQDKPASTDNTNVNGEDMAEIEENMLLPVSGQELPIRWLAQR
                     GSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLLDPLEIRDLHKLVRDTDKV
                     FPNPGNSDLGLMTAFFEAYLGADYTDCGLLTKEWMKGNRVSRITRTASGANAGGGNKT
                     DRNPNLVHTFDTLDVEIAAATLPMDFNIYEIPGSVYRRAKEIVRKKESPFKEWSAALR
                     AIPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLTETDHENPSEETLAAARHT
                     PEKDILEEVNRELAAERETEEEKNNEEKSQPSDAMADEQATAETMEPDTTEHRQDTQS
                     LDAQAQIDPVNQVKVTADEVNKIMQAANTSQPDANQLLAVSRGEFVAGISDPNDPRWV
                     KGIQTRDSIYQNQPET"
BASE COUNT          355 a          316 c          299 g          254 t
ORIGIN      
        1 ccctctgcgc aggacaaacc agccagcact gacaatacca acgtcaacgg cgaagacatg
       61 gctgagattg aggagaacat gctcctgccg gtttccggtc aggaactgcc cattcgctgg
      121 cttgctcaac gcggcagcga aaaaccggta acgcacgttt cacgcgacga actccaggca
      181 ttacatattg cacgggctga agaactaccg gctgttactg ccctcgctat ttcgcataaa
      241 accagtctgc tcgatccact ggaaattcgc gatctccata aactggtgcg tgatactgac
      301 aaagttttcc ctaatcctgg taattcagac ctgggactga tgactgcttt tttcgaagca
      361 tacctgggcg ctgactacac cgattgcggt ctgctgacaa aagagtggat gaaaggaaat
      421 cgtgtttcac gcatcacccg cacggcttcc ggtgctaatg ccggtggagg gaacaaaacc
      481 gatcgcaatc cgaatttagt acacaccttc gatacgctgg atgtggagat tgcagcggcc
      541 acacttccga tggattttaa tatttatgaa attccgggca gcgtttatcg tcgcgcaaaa
      601 gaaatcgtcc ggaagaaaga aagtccgttc aaagaatggt ccgcggcact tcgcgcaatc
      661 ccgggtatcc tggattattc ccgcgccgct atttttgcac ttatccggag cgcacaccct
      721 gagttttatc attacccggg acgtcttcag gggtatatca acgcctattt aacagagact
      781 gatcacgaga acccttccga ggaaactctc gctgctgcac gacatacacc ggaaaaagat
      841 atcctggaag aagttaaccg cgaactggct gctgaacgcg aaacagaaga agaaaaaaat
      901 aatgaggaaa aatcacaacc gtctgacgca atggcagatg aacaggcaac ggctgaaaca
      961 atggaaccgg atacaactga acatcgccag gacacgcaat cgctggatgc tcaggcacag
     1021 atagatccag ttaatcaggt aaaagttacc gctgacgaag taaacaaaat tatgcaggca
     1081 gccaatacca gccagcctga cgccaatcag ttgcttgcag tatcacgtgg tgaatttgtt
     1141 gcagggatta gcgacccgaa tgatccgaga tgggtgaagg ggatccagac ccgcgactct
     1201 atataccaga atcagccaga aacg
//
LOCUS       DAHBHC010000308         1191 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHBHC010000308 DAHBHC010000000
VERSION     DAHBHC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.313"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..810
                     /locus_tag="M2A35_005501"
     CDS             <1..810
                     /locus_tag="M2A35_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HCB7201621.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..>1191
                     /locus_tag="M2A35_005502"
     CDS             877..>1191
                     /locus_tag="M2A35_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201622.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQ"
BASE COUNT          309 a          293 c          352 g          237 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca g
//
LOCUS       DAHBHC010000309         1167 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHBHC010000309 DAHBHC010000000
VERSION     DAHBHC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.314"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..464
                     /locus_tag="M2A35_005503"
     CDS             <1..464
                     /locus_tag="M2A35_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCB7201623.1"
                     /translation="RWNTTRAGAFNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDA
                     IPAPGKSLKEIVVSAPDGAVFRYDADAGALSASGMKTATLQASVSVTLDTPVVECTNL
                     LKTAEIDVTKGGKMSGNITHSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            482..811
                     /locus_tag="M2A35_005504"
     CDS             482..811
                     /locus_tag="M2A35_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCB7201624.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            815..>1167
                     /locus_tag="M2A35_005505"
     CDS             815..>1167
                     /locus_tag="M2A35_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HCB7201625.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDT"
BASE COUNT          290 a          331 c          342 g          204 t
ORIGIN      
        1 tgcgctggaa caccacgcgc gcgggtgcct tcaatgtgtg gttgccgcca tcacccggcg
       61 aacaggtggt aatagcctgc atcggcggca acccggaaac cgccatgata attggcagtc
      121 tgtggagtga tgccattccg gcacccggca aaagcctgaa agaaatcgtg gtcagcgcgc
      181 cggatggcgc ggtgttccgc tacgacgcgg acgcaggcgc actgagcgcc agcggcatga
      241 aaacggccac tttgcaggca tccgtcagcg tgacactgga tacgcccgtc gtggaatgca
      301 caaaccttct gaaaacagcc gagattgacg tcacaaaagg gggaaagatg agcggcaata
      361 tcacgcacag cggcggcgat ttcacctcaa acggcatcac agtgcatacg cataagcacg
      421 gtggcgttaa aggtggcagc gattcgacag gaggcccgca gtgacaaccc gctacacagg
      481 aatgaatccg gacgggacgg gaaacctgaa cgatatggag cacctgaaac agtcagtcag
      541 ggatatcctg accaccccgc tggcaagccg ggttatgcga cgggaatatg gcagccttgt
      601 gcctgatttg attgacgaac ccatgaataa caccacgcgt ctgcaatgca tgagtgctgc
      661 cgtgattgca ctgacacgat gggaaccccg cattgccctg gatgccatcg acgttgtctg
      721 gaaagcggga ggccgcgccg gggtgacgct gtcgggcact gtcatgcaga ccatgcagaa
      781 tgttgaatta accatcacgc tgagggagta aatcatgccc gccgttgacc tttcacagtt
      841 accggaaccc gccatcatcg cggagcctga ctttgaagca attctggctg acacaaaggc
      901 catgatgatt gcggcttatc ccgccgaaca gcgtgaagcc gtctccgccg cgctggaact
      961 ggaatcagaa ccactgaacg ttatcgctca aaccatgtct tttcgtgaaa tgctgttacg
     1021 ccagcgggtc aatgagggtg cacgcgcctg catgttaagc cacagcgccg ggacagacct
     1081 ggacaacctc gcgggcaata tgaacacaaa gcgcctggtt atcactccgg caacggatac
     1141 caccgacgcg gtgatggaga gcgacac
//
LOCUS       DAHBHC010000310         1159 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHBHC010000310 DAHBHC010000000
VERSION     DAHBHC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.315"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>1159
                     /locus_tag="M2A35_005506"
     CDS             <1..>1159
                     /locus_tag="M2A35_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCB7201626.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP"
BASE COUNT          222 a          410 c          347 g          180 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
      961 ctatgtgctc gacggcctgc tgtaccacga gtccgacctg cggatcgagg agcactacac
     1021 cgacacggcc ggcttcaccg atcacgtctt tgccctgatg cacctgctag gcttccgctt
     1081 cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac gtgccgcagg gcgtgcaagc
     1141 ctacccgacg ttgcgcccg
//
LOCUS       DAHBHC010000311         1149 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHBHC010000311 DAHBHC010000000
VERSION     DAHBHC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.316"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            11..194
                     /locus_tag="M2A35_005507"
                     /pseudo
     CDS             11..194
                     /locus_tag="M2A35_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            241..1143
                     /locus_tag="M2A35_005508"
                     /pseudo
     CDS             241..1143
                     /locus_tag="M2A35_005508"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          374 a          221 c          264 g          290 t
ORIGIN      
        1 aaaagaagtc gtggatgact gtttttctgt tcgtgaagta gcggtaaaac acggaatacc
       61 agcctttggt actgtatgca actggcttga aaaatataga aaatatggcg aagatgcttt
      121 cattcgaaaa aataaaaaga gtattcctgt gccggataaa agcgccattt cagcaccacc
      181 actaccaaca ttaaccaaag atgagagaga agaacttgaa caactcaggg tcgagaatac
      241 ctacttaaaa aagctgaaag ccttggttca gcagaaaaca tgctcaacgt tcaggaaaaa
      301 gtgagcattg tgaatgaact aaggcaggaa tggccattgt cccgattgtt aattgtttcc
      361 gggttgcccc ggagtacatt ttactatcat gtcagacggc ttgctgctcc tgaccggtat
      421 cagtctgcca gagcgcttgt gctgaaaatt tatcaccagc acaaaggccg ctatggttac
      481 agacgcatca ggctggcatg tcgtaacgag ggggttttac ttaatggtaa aaccgtcaga
      541 aaactgatga aggaactggg tattagcagc ctgatccgcg ttaaaaaata ccgttcatat
      601 aaaggagaac aaggacgaat ttgcgataat ctcctgaaac gcaattttga tgcaaaacgc
      661 cccaacgaga agtgggttac agatgtcact gaatttaaag tgaatggcaa gaagctgtat
      721 ttgtcaccca tcatggatct ttataatgga gaaataattt cctataacct cgctactcgc
      781 cctcaacctt caatggtgca gactatgctt acggatgcgc tgaaacagct gtcgaaagat
      841 gaacacccca tactgcacag cgatcaaggt tggcaatatc agatgtcccg gtggcaacga
      901 tggttaaagg atagcggtat agtccagagt atgtctcgcc ggggaaattg tcttgataat
      961 gcagttatag agagcttttt tggaacattg aaatcagaat gttattacct caatgaatat
     1021 aaaaatgtgg aggacttaaa gcgagacatc attgattaca taaactatta taatcaattg
     1081 aggataaagg aaaaacttgg cggtcttagc ccggtacaat atcgattgag tcaggctgca
     1141 taagttgtc
//
LOCUS       DAHBHC010000312         1132 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHBHC010000312 DAHBHC010000000
VERSION     DAHBHC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.317"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            89..457
                     /locus_tag="M2A35_005509"
     CDS             89..457
                     /locus_tag="M2A35_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201627.1"
                     /translation="MAKEKLVTIHVHTPFTLTLGDQSKQEFGRGRHNVPEEVASHWFT
                     QAHSELSESVISDTDDLQPIIDGLQAQIADKDKLIADLKDALLKLQEQNDSLQAQITA
                     ARAGGNGAKDVKESKSASGK"
     gene            423..830
                     /locus_tag="M2A35_005510"
     CDS             423..830
                     /locus_tag="M2A35_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCB7201628.1"
                     /translation="MSKNQSLPAVSDFRRDFPQFADPAKYPDAQIGFRLNLADELLTE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLIMMFGAGGRQL"
     gene            827..>1132
                     /locus_tag="M2A35_005511"
     CDS             827..>1132
                     /locus_tag="M2A35_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201629.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKTAAQAALDGNAAGAESA
                     "
BASE COUNT          289 a          285 c          324 g          234 t
ORIGIN      
        1 gttgagccag tgtataaaga aactctgtcc tatgtggacg gtatctgata accagaatgg
       61 ccccgaaagg ggcctgaagg aaactgaaat ggcgaaagaa aagctggtta ccatccatgt
      121 tcacaccccg tttacgctga cgctcggcga tcagtcaaaa caggagtttg gccggggacg
      181 acataacgta ccggaagaag tcgcgtcgca ctggttcacc caggcgcact ctgagctttc
      241 cgaaagcgtg attagcgaca ccgatgatct gcaacccatt atcgacggcc tgcaagcgca
      301 gattgccgac aaagataagc tgattgccga tctgaaagat gcattgctca aactgcagga
      361 gcagaacgac agcctgcagg cgcaaattac tgccgcccgg gctggcggta atggggctaa
      421 agatgtcaaa gaatcaaagt ctgccagcgg taagtgattt tcgacgcgac ttcccgcagt
      481 ttgctgaccc ggcaaaatat cccgacgccc aaatcgggtt ccgtctgaat ctggccgatg
      541 aactgctgac cgaaaacgtc accggcaaaa agttgtttcc gtactttgcc gggttgttcg
      601 ttgcgcacta catgacgctc tgggcggcag acagcagggc gatgctggct ggtggtccgg
      661 gcggttcaac caatggtgtt cagtcctcaa agtccgtgga taaggtaagc gtcagttatg
      721 acaccagcgc gacgctgaat cctgatgcag gtttctggaa taacacccga tatggcgctg
      781 aattttatca gttgatcatg atgttcggtg caggtggtcg ccagctatga gtttcaaaag
      841 cggtgtaaca acgagggtgg ataacgctaa ggccattctg gatgcgctca ggtcgttaac
      901 caaaaaagat gtgctggtcg gcatcccttc ggaagacagc gagcgggatg atgttccgtt
      961 tggtaatgcg ggcatcggtt acctcaacga atacggctca ccagagcaga acatcccgcc
     1021 acgacctcac ctggtccccg gcgttaaatc ggcagaagag cagacggtgc cgcagcttaa
     1081 aaccgcggcg caggctgcac ttgatggtaa tgctgcggga gcagaaagtg ca
//
LOCUS       DAHBHC010000313         1124 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHBHC010000313 DAHBHC010000000
VERSION     DAHBHC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.318"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(28..561)
                     /locus_tag="M2A35_005512"
     CDS             complement(28..561)
                     /locus_tag="M2A35_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7201630.1"
                     /translation="MNVKIRYSLSAAVLALIATSASAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIGQ"
     gene            696..935
                     /locus_tag="M2A35_005513"
     CDS             696..935
                     /locus_tag="M2A35_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201631.1"
                     /translation="MQKEVFINITADCSSPASTAKEIEALKYMITVIFSVLDQNKKNG
                     IIHQLNEHVNNPYIKSNLEMLLPMKDIGKPTETKG"
     gene            complement(932..>1124)
                     /locus_tag="M2A35_005514"
     CDS             complement(932..>1124)
                     /locus_tag="M2A35_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCB7201632.1"
                     /translation="LDRVEGAALEVYEAAARRSIRQVFLDVAAGLCEGDELLPETRPC
                     SEARYTIKINSSDNSITGC"
BASE COUNT          314 a          286 c          237 g          287 t
ORIGIN      
        1 gaacggagat aatcgcggcg actctgctca ctgacctatc ccccaacagg ctaatgcgct
       61 ttcctggtca cgacgaataa cctgtccata gcagttattt gaacgtatgc gacaatcgcg
      121 cccaccatct tttatccacc agcgaatcgc ctcgcatgca cctttacgat caccggcatt
      181 cagccgctta taaaacgtcg acgggaaaca cttaccgggg ccaatgttat aggggcaaaa
      241 tgacgctata cccgctttct gtggttcggt cagtggtact ttaatattgc gctccaccca
      301 tgccagcgcc ttatcacgct caatggcatt aacctgagcg catttttcct tcgacagttt
      361 catgcccgga ataacaggtt taccatccac caccgtggca ccccggcaaa tggtccatat
      421 gccggaacca tcgcggtatg cagtggtgtg gttaccctct ttttcatcca gaaactgatc
      481 gagaatgtca ggagcagatg cactggtggc aatcagtgcc agaacagcag ctgacaggct
      541 gtatctgatt tttacgttca tggatattta tcaggattta tcggtttctg aaccctggat
      601 atgttaagca ttcagcccgt cagtggtagg acactggcgc tctggttaag gattatgggg
      661 gatgctggct gagctacccc atctgaggat ttgatatgca aaaagaagtt tttatcaata
      721 ttacagctga ctgctcatct cctgcatcaa cagcaaaaga aattgaagct cttaaatata
      781 tgattacagt aatattttcc gtattggacc agaataaaaa aaatggaatc attcatcaat
      841 taaatgaaca tgtaaataat ccatatatca aaagcaatct ggaaatgctg ctaccaatga
      901 aagatattgg aaaaccaact gaaacaaaag gctaacaacc agtaatcgag ttatctgaac
      961 tgttaatttt tatggtatac cgcgcctctg aacaggggcg cgtttctggc aacagctcgt
     1021 ccccttcaca taacccggca gcaacatcca ggaagacctg tctgatgctc cttctggctg
     1081 cagcctcata aacctccagc gcggcacctt caacacggtc cagc
//
LOCUS       DAHBHC010000314         1107 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHBHC010000314 DAHBHC010000000
VERSION     DAHBHC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.319"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..465
                     /locus_tag="M2A35_005515"
     CDS             <1..465
                     /locus_tag="M2A35_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7201633.1"
                     /translation="GVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGA
                     HDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQ
                     ERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(582..>1107)
                     /locus_tag="M2A35_005516"
     CDS             complement(582..>1107)
                     /locus_tag="M2A35_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCB7201634.1"
                     /translation="HDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRK
                     TKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENA
                     LRAVSLGRKNFLFFGSDHGGERGALLYSLIGMCKLNDVDPESYLRHVLGVIADWPVNR
                     VSELLPWRIALPAE"
BASE COUNT          278 a          282 c          283 g          264 t
ORIGIN      
        1 ggcgtaaaat tctcacaccg gggatccaag atgcgaacac cacatcttgt gccactcagc
       61 aaacaggttg tagccatact ggcagaactt cagacatggg ctggtgaaaa tggtctgatc
      121 tttacgggag cacatgaccc acgtaaacca atcagtgaaa acaccgtaaa taaagccctg
      181 agggttatgg ggtatgacac aacccaggat gtctgtggtc atggattccg ggcgatggca
      241 tgtagtgcgt taatagaatc aggtttgtgg tcccgcgatg cagttgaacg tcagatgagc
      301 catcaggaac gtaacggtgt acgtgctgct tacattcata aagcagaaca tctggaagaa
      361 cggcgactga tgctacagtg gtgggccgat tttctggatg caaacagaga aagatttatc
      421 agtccatttg aatatgcaaa gattaataat ccattaaaac agtaatcatc ccgggcaaat
      481 gcccgggaac ctactccagg attattttct ttgttaaaaa aacagacaaa acggtattaa
      541 cttcgtaagc gtacagcgag ggccgtattg acggggatgt gttattcagc tggcagtgct
      601 atgcgccacg gaagcagttc gctgacccgg ttgaccggcc agtctgctat gacgccaagc
      661 acatggcgaa ggtagctttc tggatccacg tcattcagtt tgcacatccc gatcaggctg
      721 tacagtagcg ctccccgctc accaccatgg tcagagccga agaacaggaa gtttttacga
      781 cccagactga ccgcccgcag ggcattttca gcgatgttgt tgtcgatttc cacccagcca
      841 tcgttcgcat agtacgtcag tgccggccac tggttaagtg cgtacgcgaa cgccttcgcc
      901 aactctgagt gtcgcgacag ggtcttcatc ttttcacgca accagctttc cagggatttc
      961 aacagcggtt tcgtttttcg ctgacgttca gcaagccgct gctctgccgg cattcccctt
     1021 atatccgcct ctatggcgta caactgaccg atctgctcca gggcttcttc cgtcagtgct
     1081 gacgggatgc ggacgtgcac atcgtgg
//
LOCUS       DAHBHC010000315         1100 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHBHC010000315 DAHBHC010000000
VERSION     DAHBHC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.320"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..187
                     /locus_tag="M2A35_005517"
     CDS             <1..187
                     /locus_tag="M2A35_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCB7201635.1"
                     /translation="RNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVS
                     WAISDILCRPRIFAELC"
     gene            187..387
                     /locus_tag="M2A35_005518"
     CDS             187..387
                     /locus_tag="M2A35_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCB7201636.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            390..713
                     /locus_tag="M2A35_005519"
     CDS             390..713
                     /locus_tag="M2A35_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCB7201637.1"
                     /translation="MNTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            710..>1100
                     /locus_tag="M2A35_005520"
     CDS             710..>1100
                     /locus_tag="M2A35_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCB7201638.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
BASE COUNT          262 a          267 c          349 g          222 t
ORIGIN      
        1 gcgcaatgct gtttataccg ccacacgcgc cctgattctg gagcgttacc gcgatgcgga
       61 cacaacggac aagggcgacc gccgggcgga cgcactggat atacagacat cagatttgtg
      121 gcgcgatgtg agctgggcca tctctgacat tctgtgccgc ccgcgaatct ttgcggagtt
      181 gtgctgatga aagtgaaggc actggaaggc gacaccgtgg attcgctctg tttccggtac
      241 tacggcacga cgcagggcgt caccgaaaag gtgctggatg ccaaccccgg actctgtcag
      301 caggtatttc tggacgccgg gcaggaagtg gagatgccgg agccggagaa gaagaaacga
      361 gaaatgattc agttgtgggg ggagtagcag tgaacaccat tcaaacaggg atcacagagc
      421 aggttattgc atggctcttt gaccacctgc caacggtgta tgcagtaggc gcggcggtca
      481 gcatttccgc gctgatgagt ctttatgacg gacgaacact ggttcagacc gtaacgggat
      541 cgctggcgtg cggcgttctc gccatggccg tggccgggtc gttgcgcttc ttcggttttc
      601 ctgaagatgc cgtgacgttt atcggcgcat caatcggttt tatgggggca gagaaagcac
      661 gcgacaaggt tattgcggcc tttaatcgca gggtgaagga gaaggacgaa tgagcaacac
      721 atttaaattc agcagccgga gcgaaaagaa tttgcagggc gtaaatcctg atctggtgaa
      781 agtgacccga cgggcactgg aaatttcgga agtggatttt ggtatcaccg aagggttgcg
      841 cagccgttac cgccagaagc aacttgtggc cacaggtaag agccagacca tgaacagccg
      901 ccaccttacg gggcatgccg tggatgttgt ggcttatatc ggcagccagg tgtcatggga
      961 atggccgctg tacgaaaaaa tcgcagcagc attcagacag gccagccggg aactgaatat
     1021 tccggtggaa tggggcggcg actggaagac cctgaaagac ggaccgcatt ttcagttacc
     1081 acacggagcc tatccggcat
//
LOCUS       DAHBHC010000316         1094 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHBHC010000316 DAHBHC010000000
VERSION     DAHBHC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.321"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..227)
                     /locus_tag="M2A35_005521"
     CDS             complement(<1..227)
                     /locus_tag="M2A35_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201639.1"
                     /translation="MKSIATAVSTINVPFHGAELYVVNHNGEPYTPMKPIVEGMGMTW
                     QSQHRKLMERFKTCIIEMMIQLPGDTQRRLV"
     gene            complement(290..430)
                     /locus_tag="M2A35_005522"
     CDS             complement(290..430)
                     /locus_tag="M2A35_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCB7201640.1"
                     /translation="MKVRDIAPLGIRIPPEIKEKLKEKAKEEGRSLNSEIVQRLIRSL
                     KS"
     gene            540..788
                     /locus_tag="M2A35_005523"
     CDS             540..788
                     /locus_tag="M2A35_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCB7201641.1"
                     /translation="MKGARDISPLGIRIPDYLKKRIQQEADKNGRSMNSEIVLILQKH
                     VDNLDGSRSLEGFANQEAEKFKKALLETLTKMYSKDKQ"
     gene            799..>1094
                     /locus_tag="M2A35_005524"
     CDS             799..>1094
                     /locus_tag="M2A35_005524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCB7201642.1"
                     /translation="MKIDYDYLKEVLSTFLTSDKPYITIRDLGFYEKNQEDLSKLIFH
                     LTLLVDQGIIRDRELKYSSFEDITGIYFSGDGILHGGECDIVLTSEGHDFAMAL"
BASE COUNT          320 a          230 c          231 g          313 t
ORIGIN      
        1 ataaccagac gacgctgagt atcgccgggg agctgaatca tcatttcgat gatgcaggtc
       61 ttaaatctct ccatcaactt gcggtgttga gactgccaag tcatacccat tccctcaacg
      121 ataggtttca ttggggtgta cggttcgccg ttgtgattga cgacataaag ctctgcgccg
      181 tggaatggca cgttgatagt agatactgct gttgctatac ttttcatgtc aatatttcct
      241 aagacgattt gttgataccg aagccctgac tgttccagca gttggggctt caacttttta
      301 ggctgcgaat aagacgctgc actatttctg aatttaaaga cctgccctcc tccttagcct
      361 tttccttcaa tttttcttta atctctggcg ggatacgaat ccctaatgga gcgatgtctc
      421 taactttcat ctcaccctcg cttgctacac cgtgataact aaatactaca ccgtgtttat
      481 ttaaagtcaa agtttttttg cctacactgt gtagctatgc acagcgtgca ggaggagaaa
      541 tgaaaggtgc aagagatatc agtccattgg ggattaggat tccagactat ttaaagaaac
      601 gcattcagca agaagcggat aaaaacggca gatcaatgaa ctcagagatt gtgcttatcc
      661 ttcaaaaaca tgttgataat cttgatggct ctcgctcact tgaaggattc gctaaccaag
      721 aagctgaaaa gttcaaaaag gcgcttcttg agaccttaac aaaaatgtac agcaaggaca
      781 aacagtgaag gacaacgtat gaaaattgat tatgactatc tgaaagaagt gttgtcgacg
      841 ttcttaacct ccgataaacc atacataaca atcagagatt taggtttcta cgaaaaaaac
      901 caagaagacc tgtctaagct aatttttcac ctgaccctac tggttgacca agggattatc
      961 agagatagag agttgaaata ctcttccttt gaggatatca ccggaatcta ctttagtgga
     1021 gacggaatac ttcatggtgg tgaatgtgac attgttctca catcagaagg tcatgacttt
     1081 gccatggctc ttag
//
LOCUS       DAHBHC010000317         1084 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHBHC010000317 DAHBHC010000000
VERSION     DAHBHC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.322"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(19..621)
                     /locus_tag="M2A35_005525"
     CDS             complement(19..621)
                     /locus_tag="M2A35_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201643.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            864..1031
                     /locus_tag="M2A35_005526"
     CDS             864..1031
                     /locus_tag="M2A35_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201644.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
BASE COUNT          352 a          235 c          204 g          293 t
ORIGIN      
        1 tgcccgctat atactatctc attcagtatc atcacgcgcg gtctgtgcat atgttactac
       61 cgcacctaat gtattaattt tcttttcaac atagataata ttatcgtact cataattgcc
      121 atacggatag caaatgcgaa tattctcatg tagatcgggg tcatccacct cagctccaga
      181 acaacttttt gaactaccgg aagtataccg atacggtgca acataagacg atgtctctcc
      241 aggcaaaaaa taagttagtg ttgtaagggg tataatcaga aaaaatccag caaatatgca
      301 catccctgca taaaccttaa ggtatgctga cagactcttc cagccgcttt gttttactat
      361 ccccttctta acccaaaaca gagataacag aaaagctatt cccatgctaa acagaatgta
      421 atagtgggat atactctgat taagaaacgt gaccctgtaa atatctgccc gccaccagaa
      481 gaaaaggaaa ataaagatca ggcctgaaac tgtcatgcaa atcaaataag gatacgaatc
      541 ttttttcatg tttagcgtcc ataaaatttt tcctgccccg gacaaattta ccatccattt
      601 tttgcgcaga aaatagctca ttacttactg cacaataata cacaaaattg cgtaaatttt
      661 ttgcatggat tttagctctt tcagccgacg tttaaggggt aaatagcatt tcctaaaagc
      721 aactgcacca acccaacaga atgagctacc gcttacgttg agagcaaaaa agtgtatagc
      781 agcaatgaac agcatcctcg cactgacgag gatttctttt atctgaactc gctacggcgg
      841 gttttgtttt atggagatga taaatgcact tccgagtcac aggtgaatgg aatggagaac
      901 cattcaacag agttatcgaa gcagagaaca tcaatgactg ctatgaccac tggatgatat
      961 gggcgcagat agcacatgca gacataacca atattcgaat tgaagaactg aaagaacacc
     1021 aagccgcctg atggcggctt tttcttgcgt gtaattgcgg agactttgcg atgtacttga
     1081 cact
//
LOCUS       DAHBHC010000318         1058 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHBHC010000318 DAHBHC010000000
VERSION     DAHBHC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.323"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            678..896
                     /gene="ccdA"
                     /locus_tag="M2A35_005527"
     CDS             678..896
                     /gene="ccdA"
                     /locus_tag="M2A35_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCB7201645.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVAWFIEMNGSFADENRDW"
     gene            898..>1058
                     /locus_tag="M2A35_005528"
     CDS             898..>1058
                     /locus_tag="M2A35_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HCB7201646.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVV"
BASE COUNT          265 a          241 c          246 g          306 t
ORIGIN      
        1 ccagattgca ttcccggatg cgtcgttccg caaaccgggt cagttgttca tagctgagtg
       61 aaaacacccg ttcttcatcc atcctgcacg ctccatctga taatattctg acaggtaaaa
      121 aatcatatcc tgcatgattt gtctgttatt gatttatgat aatatctagt atggttttat
      181 catcaatgat aaaaatcaca tctgacatga ttttatgtaa attgcttaat tatcatattg
      241 cctgcccgtt ccccgccgcg cagatcctgt ttccggaagt tccggatccg cggatccttc
      301 gctgccgccc gccggcccgc tttcacatta tgtggtgccc agctctgctg acatgacact
      361 gtatgttgtg tttcttcccg tgttcccgag ccagtcatca gcgacagaaa agatcgcttc
      421 gcttattcac tgatcgtgat tcatttccgg aaaatcttga atacgataaa aatcggatca
      481 tggaaaaaca gtcatcctgt aatttagcat ggttactaca tttccggcta ttattgccat
      541 gatcactttt tatgttggtt attgatgtga gtgttaataa tagcctgata acaatattcc
      601 gttgccggag gcatgctata gcgtaatact ggtgtatact taattgtgtg cagagagtat
      661 gtaagatgag gtgaattatg aagcagcgca ttacagtcac cgtcgacagt gacagctatc
      721 agttgctcaa ggcatatgat gtcaatatct ccggtctggt aagcaccacc atgcagaatg
      781 aagcccgtcg tctgcgtgcc gaacgctgga aagcagaaaa tcaggaaggg atggctgagg
      841 tcgcctggtt tattgaaatg aacggctctt ttgccgatga gaacagggac tggtgaaatg
      901 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtccag
      961 agtgacatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag cgcccgcctg
     1021 ctgtcagaca aagtctcccg tgagctttac ccggtggt
//
LOCUS       DAHBHC010000319         1036 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHBHC010000319 DAHBHC010000000
VERSION     DAHBHC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.324"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(169..438)
                     /locus_tag="M2A35_005529"
     CDS             complement(169..438)
                     /locus_tag="M2A35_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201647.1"
                     /translation="MRAKTGICKNPHRYNPTFLSLPEYQGQEGRHKCAACAFELGIKD
                     ALEGRAMAQNDLVLANIPFSQAGTVRHRDAYEAYVRGWRLIHSNN"
     gene            complement(803..1036)
                     /locus_tag="M2A35_005530"
     CDS             complement(803..1036)
                     /locus_tag="M2A35_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="partitioning protein"
                     /protein_id="HCB7201648.1"
                     /translation="MDSLTPEARKSGQRVAFLAGLALLKTNPAEAYRLAAWADDEMLP
                     VTQISSKKFEAQSAPVAKITSQMAGNIRALFPE"
BASE COUNT          279 a          239 c          230 g          288 t
ORIGIN      
        1 cccgtcacct gcgcgcagaa aaaacgcatt tttttgtgca cgcacggatc cttgacggat
       61 ccagccgcca tgcgggccgg aagtacaaaa agtcgttcaa aaaaattgtg caaacttgtg
      121 cactatcgtg caaacaaaaa aaagcgcctt atcggcgctt caaaagtatc aattgttgct
      181 gtgtattaat cgccaaccac gaacatatgc ttcataggca tctctgtgcc ttacagttcc
      241 agcctggcta aacggaatgt tagctaaaac taaatcattc tgagccatag cgcgtccttc
      301 aagcgcatct tttatgccta gctcaaaagc acaggcagcg catttgtgcc gaccttcttg
      361 tccttgatat tcagggagag acagaaatgt tgggttataa cgatgagggt tcttgcaaat
      421 acctgtttta gcccgcactt tatttacctc ataggaaaaa tatgcgtgcc tttacagagg
      481 tgcgtgtaag caataaaata tacgacgcac attttgattt acttcggaag gcacgcatat
      541 caggttaagt gagtccgacc gagttttacg ccccaatagt tgctgctatt ggggcgtttt
      601 gcatggacaa tgccgcgcaa ttatcttgtc gctcacaatg cgaacgatct tacaaaaagg
      661 cacattactg tcaagataat tgatcgtttt aatcgataga taatagacaa tctatttgtt
      721 taacagatcg attattgaag taagtgtgcc aaatggaatg atactgtttc tataaacgtg
      781 agcatttttc gtgcagatgc ttttactcag gaaataacgc ccggatattc ccagccatct
      841 ggctggttat cttagccact ggtgcagact gtgcttcaaa cttttttgag ctgatttgtg
      901 tcacaggtaa catctcatca tcagcccatg cggccagtcg gtaagcctct gccggattcg
      961 tcttcagaag tgccagcccg gccagaaaag ccacgcgttg gccgcttttg cgggcttctg
     1021 gtgtaaggct gtccag
//
LOCUS       DAHBHC010000320         1030 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHBHC010000320 DAHBHC010000000
VERSION     DAHBHC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.325"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            61..852
                     /locus_tag="M2A35_005531"
     CDS             61..852
                     /locus_tag="M2A35_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCB7201649.1"
                     /translation="MANIKEIYYTDSGPDCGGSGFPVIVLIPDLLMTAGMFRKQKIFF
                     RRKYRVITIDYHDRLLLSAESSEAFLKNITEEIIHLMDVLKVSRFILCGVRMGGFIAL
                     NAAAEYGDRVCGMIVSGMWCEQSSRGCNQRYLALSNIHLTHRKRNKIISSILRDISGD
                     DEDEFRYCKDIWTSYSPTSLSRVLSRMTSSVSVLSSTERVYQPVLVMHGGEDNEVPVQ
                     MATDLAAGFRYSRLTVIPGGGHIFTHTHGRTTNNVIQFWLNDYFC"
BASE COUNT          265 a          199 c          253 g          313 t
ORIGIN      
        1 aacagatata acatgaccac atattgtctg ttatgtgaaa acccacaggt tttataaagt
       61 atggcaaaca ttaaggaaat atattataca gattcaggtc cggactgtgg gggctccggt
      121 tttcctgtta ttgttcttat tcctgacctt ctgatgactg ccggaatgtt cagaaaacaa
      181 aaaatatttt tccgcagaaa ataccgggtt atcaccattg attaccatga ccggctactc
      241 ctgtcagcag aaagttcgga agcatttctg aaaaatatca cagaagagat tattcatctt
      301 atggatgtgt taaaggtttc gcggtttatt ctctgtggtg tccgtatggg gggattcatt
      361 gcattaaatg ccgccgctga gtacggtgac agagtctgtg ggatgattgt ctcagggatg
      421 tggtgtgagc agagctcccg gggatgtaat cagaggtatc tggcactcag taatattcat
      481 ctgacacacc gtaaacgtaa taaaatcatc agcagtattt tacgcgatat ctccggtgac
      541 gatgaagatg agttcaggta ctgtaaggat atatggactt catattcccc gacatcactg
      601 agccgggtat tatcacggat gacgtcatcc gtcagtgttc tgagcagtac tgaaagagta
      661 taccagcctg tactggtcat gcatggcggg gaggataatg aggttcctgt tcagatggct
      721 acagaccttg ccgcagggtt caggtattcc cggctgacgg ttatccccgg agggggacac
      781 atattcaccc acacgcatgg ccgcacgacc aataatgtta ttcagttctg gctgaatgat
      841 tatttttgct gagagacagc tgtctggttg agaattaaag atttttatgg gatatcttta
      901 cattattgct gttgttttac tgatttatat gttctttgtg gtgggatgga ttctgtttat
      961 tgtcagcgac ctggactcag tgtctgactt actccgcttt atgctcaggg gaatgctgaa
     1021 ctgtctcagt
//
LOCUS       DAHBHC010000321         1019 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHBHC010000321 DAHBHC010000000
VERSION     DAHBHC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.326"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..281)
                     /locus_tag="M2A35_005532"
     CDS             complement(<1..281)
                     /locus_tag="M2A35_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCB7201650.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDPKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDAEG"
     gene            complement(430..780)
                     /locus_tag="M2A35_005533"
     CDS             complement(430..780)
                     /locus_tag="M2A35_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCB7201651.1"
                     /translation="MPSLIPRACRKRGCAGSTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            complement(885..>1019)
                     /locus_tag="M2A35_005534"
     CDS             complement(885..>1019)
                     /locus_tag="M2A35_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCB7201652.1"
                     /translation="SACGSMPVAPQPCVKPPDPPAWIMQPAPDWQTPLNGIISPSESG
                     "
BASE COUNT          208 a          276 c          266 g          269 t
ORIGIN      
        1 ccctcagcat cgagtgtttc acagtgatgc cggtattcgg tgtaggcttc caccagtaac
       61 tcgagcgcac gtgcatcgag ctgagaaatg atcccttccg cattcaactc ttccgccatt
      121 cgcctgaacc agtacttccc ctgcgcccct aaatgttgcg gaattttagg aagacctttt
      181 tcgtcctttt tagcggtttt tttggggtct ttaacgggac gttttgaggg gttgcctcgt
      241 atcaaatgca ggcgtggcgg ggttttcggg ggtcctgaca taatcggttt tacctatcaa
      301 tcgtttaatc acattcccaa aaaaaagttt tcgaacctgc ggcgatgcga ggaagggtca
      361 ggcggcggta ctgagcagcc agggctgcag agatttgacc cgcccctccc ctacagatgg
      421 gaactgttat caattgatgc gttcgcgcgc tgtttttgct ttatggcagg gccagcacag
      481 actctgcagg ttactgtctg catctgtgcc accatgagct ttcggaatga tgtggtccac
      541 agttctggct tcaacggctc tcccattgcg caggcagttc tgacacagat gattatcacg
      601 cttcagtata cgcgcacgta tggcatccca tttcgagcca tagccacgct ggtggcgact
      661 cagtccgcgt tgatgctgta cccatccttc gccacgatgt ttatcgcagt aaccagaact
      721 gtctgtggtt gaacctgcac atccacgctt acggcaggca cgtggaatta gtgatggcat
      781 aaatacctca taccctgcga aatgtttacc acgataaaaa ggctacttaa tgcactgagt
      841 gcggatatac tcctgtgccc cttccagttg catctgcatc gtcatcagcc gctctctgag
      901 ggtgaaataa tcccgttcag cggtgtctgc cagtcggggg ctggttgcat tatccacgcg
      961 ggtgggtccg gtggcttcac gcacggctgc ggagcaactg gcattgaccc gcaggcgct
//
LOCUS       DAHBHC010000322         1016 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHBHC010000322 DAHBHC010000000
VERSION     DAHBHC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.327"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>1016)
                     /locus_tag="M2A35_005535"
     CDS             complement(<1..>1016)
                     /locus_tag="M2A35_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCB7201653.1"
                     /translation="GSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQR
                     LFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHLTAE
                     VTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLAL
                     GNHRLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQ
                     FEFWFSEKRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYVRSVNTVGKSAFV
                     EAVGQASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWQDANDKW
                     NAMW"
BASE COUNT          192 a          335 c          258 g          231 t
ORIGIN      
        1 cccacatggc attccactta tcgttagcgt cctgccactc tttcgaaaac tcctccagtt
       61 tgctggcgtt atcctccgtc agctcgactt tttccagcag ctccttgccg agatgggatt
      121 cggttatctg gcctttgaaa aaatccaggt aaccttccgc atcatcgctc gcctgaccga
      181 cagcctccac gaatgccgat ttgccaacgg tgttcacact gcgaacgtaa aaataataat
      241 catggcccgg tttgatattg atactggcgg ctatccagta cagccccgtg ccaagatagc
      301 gggctgtggt ttcaacctgc ctgatatcgg taatccgctt ttccgagaac cagaactcaa
      361 actgtaccgt cgggtcataa acggcaagat gcggcgtggc ggttatctga aaatagcccg
      421 gcgtcagctc aatcctcgac ggtgctgccg gtgcggcaat ccggaacgat accgacgccg
      481 gatccccctg ctgcccccgg gcatttactg cccggactgt cagcctgtgg ttccccagcg
      541 ccagctgcgt gaagcggtat gtggtttccg tcgtccgggc cgtgctgacc agccgctcac
      601 tgccgtcatc cgctgtcacg gtcagacgga gcaggaagct cacgcccttc accaccttcg
      661 gcgtgtccca tcgagccagc acctgatact ccccgctgtc tgcagtgact tcggcagtca
      721 ggtgctgcac cgctggcggc gtgacaccgt tcaccgtgcc actctgttcg ccgtcaaagt
      781 gcgccccgtt atccacgatg gcttcttttt ccggtacatg ctgcacggcg gtgatggcat
      841 acgtgccgtc atcgttctca cggatactca cacagcggaa caggcgctgg cgcaacgttg
      901 gcaacttcag cccccacacg ctgtattctg caacgccgtc aggaacacgg ctcactttta
      961 ccttcacgcc gtcggtgacg gactggacct ccacgctgac cggattgcca cttccg
//
LOCUS       DAHBHC010000323          991 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHBHC010000323 DAHBHC010000000
VERSION     DAHBHC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.328"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..266
                     /locus_tag="M2A35_005536"
     CDS             <1..266
                     /locus_tag="M2A35_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCB7201654.1"
                     /translation="DAQELKNARDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQR
                     RFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYIEQSG"
     gene            241..>991
                     /locus_tag="M2A35_005537"
     CDS             241..>991
                     /locus_tag="M2A35_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7201655.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLK"
BASE COUNT          228 a          249 c          291 g          223 t
ORIGIN      
        1 ccgacgcaca ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct
       61 gtactttcgt gttgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      121 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      181 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      241 gtgaatatat cgaacagtca ggttgacagg ctgcggcatt ttgtccgcgc cgggcttcgt
      301 gccctgttca ggccggagcc acagaccgcc gttgaatggg cggatgccag ttactatctc
      361 ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
      421 atgaatgcga tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc
      481 ggttattcca aaatgctgtt gggtgtttat gcctacttca tagagcataa gcagcgcaac
      541 acccttatct ggttgccgac ggatggcgat gccgagaact ttatgaaaac ccacgttgag
      601 ccgaccatcc gcgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac
      661 cgggataaca cgctcaccat gaagcgtttc accaatgggc gtggtttctg gtgcctgggc
      721 ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt
      781 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt
      841 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
      901 tgtcagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
      961 ccgcactgcg gggaggagca gtatcttaaa t
//
LOCUS       DAHBHC010000324          969 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHBHC010000324 DAHBHC010000000
VERSION     DAHBHC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.329"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>969)
                     /locus_tag="M2A35_005538"
     CDS             complement(<1..>969)
                     /locus_tag="M2A35_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCB7201656.1"
                     /translation="ELWTQINNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAI
                     QQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRVA
                     FINPANGNTTPALVTQGGQTFINEALIKYLTAPTITSGGNPPVFSLTPDGRLTAKNAD
                     ISGCVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVT
                     ISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDT
                     QGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMK"
BASE COUNT          206 a          254 c          235 g          274 t
ORIGIN      
        1 ttttcatcac cacaaccagc agatcgctga tactggttgt tggtgtccag ttattcgctc
       61 ctgatgaaga tacggtgaat gtcagtgtca gcgtcccctg tccggcaggc atatctataa
      121 ctgaggaaaa tacgccctga gtatccgtcg tggactgatt aaaaatctcc tgaccattgc
      181 gggtcactct taaccggcag gttgaatacc agtatgactg ttggttatta ctgttgaaat
      241 tctcatgctt accaccgcgg aataacactg gcggtatcat gacctgtcgg tcaaacttct
      301 gatcatcact aatcgtgacc gtaatggtgc cgctggcata agtgctcgtg cgggggaaag
      361 acttgctgac cgttttgaca atatcgcctt caatctggtt ggctgacagt ttccctttaa
      421 tctgacagtt ctcatttatc gtaacgttgt tgagcgtccc tgagttcgca ttcacacagc
      481 cactgatatc cgcattttta gcggtcagcc gcccgtccgg tgtcagggaa aataccggag
      541 gattgccgcc gctggtaatg gtgggagctg taaggtactt aatcagcgcc tcattgatga
      601 acgtctgtcc accctgtgtg accagtgcag gggtggtgtt tccgttcgcc gggttaataa
      661 aagccacacg gtccgccgcc agcagcacct gactctgcat gccgtcaggg gtgttctcaa
      721 taccggcacc gatacccgca atatagaggc gtccgtcctg catctgctgc agcttcactg
      781 cccacatgct gttcaggtta ttatttgtat caacctgaac cttctgtatc tgctgaattg
      841 ccgcactctg gtcttccagt ttcttattga cggtctgtgt gatttcattg ctgacatccg
      901 taatggacgt cctgatttca gccaggtcag gcgcaagctg accgttatta atctgcgtcc
      961 acagctcct
//
LOCUS       DAHBHC010000325          929 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHBHC010000325 DAHBHC010000000
VERSION     DAHBHC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.330"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            59..379
                     /locus_tag="M2A35_005539"
     CDS             59..379
                     /locus_tag="M2A35_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201657.1"
                     /translation="MLQSSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAID
                     KNMITILARLDSIKESLTNKPSSDAVDRKISDAKLAVLLGVPAIIAVGTGLYKLSMYF
                     FFSA"
BASE COUNT          276 a          226 c          189 g          238 t
ORIGIN      
        1 ttgtttgcac tccagtgcgt cttcccagct agcaggtata tctggaaaaa gatgggacat
       61 gctacaatca agtggtagca gtgggtctgg gagtgataac atgcctgaaa aagttgcaaa
      121 actggagtcc gatgttgctc atatcaaacg agacgttgat gaacttaaaa cagacgttaa
      181 agctatcgac aaaaatatga taactattct tgcacgtctg gactctataa aagaatcctt
      241 aacaaacaag ccatcaagtg atgctgtcga tagaaagatt tcagacgcaa agcttgcggt
      301 attgcttggt gttccagcaa tcatcgctgt aggaacagga ctttataagc tatcaatgta
      361 ctttttcttt agtgcttaag aaaatgctcg cacccttgca ttaggtaaag cgatttttca
      421 ttccccatta ccctcgctct taaccaatca tctaaacccg gccaccgtgc cgggttttct
      481 tttgccctcc cctcatcata caccgttcaa aaaaaccacc acgacctcgc ttcagttatc
      541 gctatgcgat gcaagtcaca aaataaatcc atcttaaata caaccagtta tatctaaaac
      601 aaccaacaaa acaacttttg ttgttgacgg taaaacaact atagttttaa atgagttcat
      661 cgcaacaaca caacgatacg gcaatcacct gattcaccgt tgcgatgacc gcttagatcc
      721 gcagcttgaa tttcagcagg ctccggggag tgcgaggggt gaagcggacg cgtgaacgtc
      781 ggtgtgacca gctgaaatca actcaacact tcatacctca gtcgcttcaa cgaggcggct
      841 tagttatgac aaccggcggc catccaccgc ctgaatacgc gcagaagtct ctatatgttc
      901 agcagcccag cttacgggca ggagttttt
//
LOCUS       DAHBHC010000326          911 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHBHC010000326 DAHBHC010000000
VERSION     DAHBHC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.331"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(36..239)
                     /locus_tag="M2A35_005540"
     CDS             complement(36..239)
                     /locus_tag="M2A35_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201658.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGKND"
     gene            complement(327..>911)
                     /locus_tag="M2A35_005541"
     CDS             complement(327..>911)
                     /locus_tag="M2A35_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCB7201659.1"
                     /translation="IEWGKLFNVSAMKATVNASSTDTTKIFKEFDQIATNIIDRAQSQ
                     AAAVERIVVFCKPDAFSAIRFSAGMANAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPG
                     TNIDVVKLVDPLHLAHMTADAVAVPKFAKGSNVYQNIYGAASSTFELINAAPAEVYSY
                     SYESSRGDAVNVVTENSQMVVNHGVGFSVQITVK"
BASE COUNT          276 a          203 c          170 g          262 t
ORIGIN      
        1 agaagtgtta atttctaaca tttaaaatcc ccttattaat catttttacc accttcttta
       61 cttaagaaat ggtgtagagc ttgatcaata ctctcgttta gtctgaacat acgggctttt
      121 actccttcat atggaacatc taggagagaa catagatatt tcagatttaa caatcctcta
      181 gaatcattag catctaaatg tacgtgtaca ccattttttg atttcagaat cacttccata
      241 ttttaatcac ctctcaaaat ttaaaaaata aataaaaaaa aggcgaagcg tgaatacacg
      301 ccacgcctct attacacgtt aaaatgttat ttaacagtga tttgaacaga gaagccaacg
      361 ccgtggttta ccaccatctg gctattctct gtaacaacgt taacagcatc accacgagaa
      421 gattcatagc tatagctgta cacctcagca ggagcggcat taatcagttc aaaggtgctg
      481 gaagcagccc cgtagatgtt ctgatataca ttagagcctt tagcaaactt cggaacagct
      541 acagcgtctg cggtcatatg tgcaaggtgt agcggatcta ccagtttaac aacatcaatg
      601 ttagttcctg ggatagtgaa cgctgtcacc cctggcagca ggtcacgacg ctgatacaca
      661 acattcccct cttccagtgg gcttacatac tggaatgcgt ttgccatacc agcagagaat
      721 cgaattgcag agaaggcgtc aggtttacag aaaacaacaa ttctttctac agcagccgcc
      781 tggctttgtg ctctgtcgat aatattcgta gcaatctgat caaattcttt aaaaatcttc
      841 gtggtgtcag tgctggaagc gttcacagtg gctttcatag ctgatacgtt aaacagcttg
      901 ccccactcga t
//
LOCUS       DAHBHC010000327          908 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHBHC010000327 DAHBHC010000000
VERSION     DAHBHC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.332"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(104..430)
                     /locus_tag="M2A35_005542"
     CDS             complement(104..430)
                     /locus_tag="M2A35_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201660.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(430..849)
                     /locus_tag="M2A35_005543"
                     /pseudo
     CDS             complement(430..849)
                     /locus_tag="M2A35_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          286 a          201 c          147 g          274 t
ORIGIN      
        1 ccgggtgcag ctccgggtcg ggcaggcggt tacctgccgg gtctgtggca tacgggatat
       61 caatgcccag gttacatata ataataagag caattaactt aatttatttg tttataatgc
      121 gcttacttaa tacaaaccat tgaatatacc ctcctgacag aaaataaagc catattatta
      181 tcgccataat atacttacta tcaatcctta cgccaataat gtctgataca gtccctataa
      241 atattagagc aataaagaat actatgcctg ttggaaaaca tattaccatc attccataag
      301 acatgagact aattaactcg ctcacatcac gagtggagtc taatagtgaa agtctatgca
      361 tggaaaagaa tagtattaaa agcgataaag ccagccatat agctctgaat aaaaaaataa
      421 tatatttcat tacatcactc cggtgaatac aaatctaata aaacgtccaa tgttatttcc
      481 ggagcacttc atcttacatg tttctaaaga atcattagac cagtcttggc agtttttaaa
      541 tagccctaaa tttataggtg tgaatatatc aggcaataca gaataacgcg gccttacctc
      601 tttaaacttc ccttttaaac agttctccac acaactatta tcggtttcaa cgaggtcaca
      661 acggccagca gcttcttttg tatcattact tgctttacct ggcccccata gaccatctcc
      721 ttttgattat ccccgttggt cttggccacc acaagtatag tcccctttac catctgggac
      781 acaaagatat tgatgataga acggattccc ccatatatct ggaccgcttc tttcaccagt
      841 tccgcccatt gaatgcagtg gcttaatgca ttttagtgtt tctaatccta atggatctat
      901 atttgaga
//
LOCUS       DAHBHC010000328          900 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHBHC010000328 DAHBHC010000000
VERSION     DAHBHC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.333"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..91
                     /locus_tag="M2A35_005544"
     CDS             <1..91
                     /locus_tag="M2A35_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flhB HrpN YscU SpaS family protein"
                     /protein_id="HCB7201661.1"
                     /translation="VQDEQLSSEDKFIEGEDTKIENKDDNLKN"
     gene            complement(152..445)
                     /locus_tag="M2A35_005545"
     CDS             complement(152..445)
                     /locus_tag="M2A35_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCB7201662.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(516..>900)
                     /locus_tag="M2A35_005546"
     CDS             complement(516..>900)
                     /locus_tag="M2A35_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HCB7201663.1"
                     /translation="LLASQKEYYSDLVALVDLGHSFVVIDEHQHRNLKPNTEPVNILL
                     SNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEIDILRLCVSYSLKQIAII
                     KGIDYKTISYHKIRALNKLNIKGTV"
BASE COUNT          288 a          142 c          159 g          311 t
ORIGIN      
        1 tgttcaagat gaacagctct cttcagaaga taaattcatt gagggtgaag acacaaaaat
       61 cgagaataaa gacgataatt taaaaaatta aacatctgtc gtagtcagaa atttataagg
      121 atctacacgc aaaaagcgtg tagatcctta tttaatgagt taaacgatct tgtgaaatga
      181 taagcaattg atacatactc atctctttat aagctggaac tgtagtaacc tgtgcaaatt
      241 cgcgatatag cttttgctcc agttctgctg atgatatata ttccgggtgt tcgctaaaga
      301 actgtcttgc atctactatc gcttgggatt cgccacttct tttattttga ttctccttta
      361 atgaattttc tctggtgcat gcagagagca acgatatcag cgcgatggtt attatgatgt
      421 tactattata tcctactaat gacatatcaa ctttcactta cgcaagattg gagtttaaaa
      481 taatgtttat cccattcaca gagtgctata aacaactaaa cagtaccttt tatgttaagt
      541 ttatttagag ctcttatttt gtgatatgat attgttttat aatcaattcc tttaataatg
      601 gctatttgtt ttaacgagta agaaacacat aaccgcagga tatctatctc atcatgggta
      661 agtggttctt gtgtggaata cacattttgt gtatgaagat ttgaaaccag gaaatgcgtt
      721 aaatcagata atgtaatatt cttattgatc ctgatgaaat tgttgctcaa aaggatgttt
      781 acaggttctg tatttggttt gagatttcga tgttgatgct catcaataac aacgaacgaa
      841 tgtccaagat caaccaacgc aactaaatcg ctgtaatatt ctttttggga tgctaataat
//
LOCUS       DAHBHC010000329          891 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHBHC010000329 DAHBHC010000000
VERSION     DAHBHC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.334"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..364
                     /locus_tag="M2A35_005547"
     CDS             <1..364
                     /locus_tag="M2A35_005547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201664.1"
                     /translation="AIREVARVRADFDDFDGDRRGIGDCLNESEQELIVTINKYASQI
                     AAKPPQEEKSGNADCTATSGKTDNPQASGKQVDELTMLVKRLASSLKSVNKSSKLPDK
                     AMDYLLRNGLVSVEGRGR"
BASE COUNT          234 a          167 c          255 g          235 t
ORIGIN      
        1 cgcaatccgg gaggttgcca gggttcgtgc cgatttcgat gattttgacg gtgacaggcg
       61 aggtatcggt gattgtctga atgagtctga gcaagagctt atcgttacca ttaacaaata
      121 tgccagtcag attgcagcaa agccgccgca ggaggagaag agtgggaacg ctgactgtac
      181 cgccacttct ggtaaaactg acaatccaca agcatccgga aaacaggttg atgaattaac
      241 catgttggtt aagcgattag ccagttcgtt aaaaagcgtc aataaatcaa gtaagctgcc
      301 tgataaggcg atggattatc ttttgcggaa cggactggta agtgtggagg ggaggggacg
      361 atgacctggc ctgaggcatt taccacggta gggattgcaa tggccgtggc gttggtggtg
      421 tattcgattt gccgctgggg gtaaggtatt aaagaatgcc cccgcacaca taagttgagc
      481 gtctgaggcg tggggatagg gagttagtta aattgttata caacaatatc tgattttgta
      541 ttgaagcaag gaagaggttg ccacatattg aatcatgcac gtgtacgtca aatgtgggag
      601 gtcatcgtgc tggttctgaa atgtgcgctg gctattgcgg ctgtaatggc aatttattgt
      661 cttgttatcg ttcttatgga tcacctttct gattgatttt atattggcga ggtgacggga
      721 gttaagtaga attgctgcgg gtgcttgagg ctgtctgcct caggcatgaa caccaaaagg
      781 cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa cattaatctg
      841 aggccatatc tatgctctac acacgtaggt tagcctctta cgtgccgaaa g
//
LOCUS       DAHBHC010000330          876 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHBHC010000330 DAHBHC010000000
VERSION     DAHBHC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.335"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(148..630)
                     /gene="ybiA"
                     /locus_tag="M2A35_005548"
     CDS             complement(148..630)
                     /gene="ybiA"
                     /locus_tag="M2A35_005548"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCB7201665.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
BASE COUNT          212 a          222 c          207 g          235 t
ORIGIN      
        1 aactctgcaa tatattgaat ttgcatgctt ttgtaggccg gataaggcgt ttacgccgca
       61 tccggcattt cacaacaagc acttgtcagc aatttgagaa cacgggaaaa aatttatctg
      121 ttttaccgcc cataagccac cgagacgtta cttctctata gccaattgtt cgcgcaactc
      181 cattaaaagg tagcccagtc gattcttgcc cttaccatta ccaccgtctc cccagtaagc
      241 atcgttttcc gtatgctcaa ccagttttgc gggcgcggtt gccagcaaga gcgcacgcag
      301 ttctggatgc tgttcgaatt tagcgcgaag tgcttttcgc atcacctgtt ctttgaccga
      361 ctcccagttt ttacgcagag gcttagaacg atcgcgcccc atgcgtgcgg cgaccatagg
      421 ggaagaaacc cgacggatct cttcgcggta tttttcatca aggaattttt gtgcctgaaa
      481 atagtgttct gaggtgggcc aggttttccc gtcaaccttg atgggccagg cggcaaagtt
      541 agaaaaatca ccgtagtcat cgctggtgct gtagaaattt atgatggtgt cttgcatgac
      601 gtgttggatc ctttgtgctc gaacgggcat taaaccgcat tatgttggtg gttattgcga
      661 gccgctttcc agaaacagaa aaaccattac ccctgaaaac cgaaaaatgc cacaatattg
      721 gctgtttata cagtatttca ggttttctca tggcattaac cgccgcgctt aaagcgcaaa
      781 ttgccgcctg gtataaggcg cttcaggaac agatccccga ctttattccc cgtgcgccgc
      841 agcggcagat gattgcggac gtcgccaaaa cgctgg
//
LOCUS       DAHBHC010000331          866 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHBHC010000331 DAHBHC010000000
VERSION     DAHBHC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.336"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            3..173
                     /locus_tag="M2A35_005549"
     CDS             3..173
                     /locus_tag="M2A35_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCB7201666.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEAWQPFGSPVDITPYTLM
                     QAIAAEGYVVVK"
     gene            793..>866
                     /locus_tag="M2A35_005550"
     CDS             793..>866
                     /locus_tag="M2A35_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCB7201667.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLL"
BASE COUNT          234 a          175 c          233 g          224 t
ORIGIN      
        1 cgatgtcgat taaacattac gatgttgtca gggcggcgtc gccgtcagac cttgcggaaa
       61 agctgacaca caaactgaaa gaggcctggc agccgtttgg tagtccggtg gacataactc
      121 cttataccct gatgcaggcg attgcagcag aaggttatgt ggtggtcaag taatactggc
      181 caccgttacc cagaaactga atggagttgc aggttcggca ctccaggtca taaaagacag
      241 tctgacactg agcctgaatg cgctgacgct gacggatgtt acaaaaagtg cagcgtatgg
      301 cgttgagata gaaagtctgg tgctggagat gaatgcaccg gcagcataat aaaaaagagc
      361 cagcgcccac tctgaaggac gctggctaaa aggccttata tgtttgtaga gacttatttt
      421 tcacagacag caatgatgtc tgtcaatata ttatcaatat gcggattgtt tcagttacag
      481 atgctttatt aaagaaaaaa acagccagca ctgactttcg gtggagaggt gctggctcaa
      541 aaggatagat ggacttcaca tgttgcttct atatggcggt acattttctg acagacagtg
      601 acggatgttg tcaagatatt gtgtcattta taacctgaat caggggaggc cggaatgtta
      661 tctggcattt ttagcagagc ctgaatgcca taatcacggc tcccggagtt ggccgtcagt
      721 gggtgacact ggcggctttt ttgtttttct ttactttcat tttctgtcgg cggtgacgga
      781 gacatacatc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc agcggtgggg
      841 acgggatact ggttactgca gctgct
//
LOCUS       DAHBHC010000332          863 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHBHC010000332 DAHBHC010000000
VERSION     DAHBHC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.337"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            65..484
                     /locus_tag="M2A35_005551"
     CDS             65..484
                     /locus_tag="M2A35_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCB7201668.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRISRRLPVTTTSDAGLELLPVEITPDEPKEPMAALTPPLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            481..831
                     /gene="tnpB"
                     /locus_tag="M2A35_005552"
     CDS             481..831
                     /gene="tnpB"
                     /locus_tag="M2A35_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCB7201669.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNVNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
BASE COUNT          223 a          215 c          212 g          213 t
ORIGIN      
        1 tatgtagcca ttagacaaga attggtaact tagacgccca tctgacacag acggacatct
       61 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aagtattcga atgaattcaa
      121 acttcgtatg gttgaactgg catcacaacc cggtgcttgt gtggctcaga ttgcacgaga
      181 aaatggcgtc aatgataatg ttattttcaa atggctcaga ctctggcaaa acgaagggcg
      241 tatatcacgg cgtcttccgg tgacaaccac ttctgacgca ggccttgaat tattacctgt
      301 agagataacg cctgatgagc cgaaagaacc gatggctgct cttactccgc ccttatccac
      361 tcagactact gtcagtgcca gctcctgcaa ggtggagttc cgtcacggta acatgacgct
      421 ggaaaatcct tcaccagagc tgctcactgt attgattcgt gaactgaccg ggaggggaag
      481 atgatctcac tcccatccgg tacccgtatc tggctcgttg ccgggataac cgatatgcgt
      541 aagtctttca acgggctggg tgaacaggta cagcatgtgt taaatgttaa tcccttctcc
      601 ggtcacctgt ttatcttccg tggccgacgg ggtgacaccg ttaaaattct ttgggctgat
      661 gctgatggtc tgtgcctgtt caccaaacgt ctggaggaag gccagtttat ctggcctgcg
      721 gtgcgtgacg gtaaggtatc cattacccgc tcgcaactgg caatgctcct cgataagctg
      781 gactggcgtc agccaaaaac atcccgcctt aacgccctga caatgttgta aaaaacgcct
      841 ggccgcatta taaaaacggc cat
//
LOCUS       DAHBHC010000333          846 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHBHC010000333 DAHBHC010000000
VERSION     DAHBHC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.338"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..458)
                     /locus_tag="M2A35_005553"
     CDS             complement(<1..458)
                     /locus_tag="M2A35_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCB7201670.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEV"
     gene            complement(514..846)
                     /locus_tag="M2A35_005554"
     CDS             complement(514..846)
                     /locus_tag="M2A35_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCB7201671.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
BASE COUNT          187 a          229 c          244 g          186 t
ORIGIN      
        1 tccacttcaa ccggatcgaa ggcttcaccg gtcatggtgt atttgccctt aagcacggca
       61 gaaactgcct gcatctcttc gacctgagca atggccagct cttcgtctcg catgttctgc
      121 aggatgatgc gacggcggcg gtaagccggg tccgccagat tctgtggatc ttcatccggc
      181 aggcgacgca gggtcatctg cggattcact tcatgcttgg gtttgacata tcccggcgta
      241 aattcagagg tggagccgcc acgggagcgg ataacctcac cggaaacaat cggcgaaacg
      301 tacagcgcca tgtttaccag tcccggaatt tgtgagagat agactttctc cgtggtgaag
      361 ggatagctct cacggaaaaa gagacgcaga aacagcggat caaacttaaa tttctgctca
      421 tttgccgcca gcagctgggc ggttgtgtac atcgacataa aaaaatcccg taaaaaaagc
      481 cgcacaggcg gcctttagtg atgaagggta aggttaaacg atgctgattg ccgttccggc
      541 aaacgcggtc cgttttttcg tctcatcgct ggcagcctcc ggccagagca catcctcata
      601 acggaacgtg ccggacttgt agaacgtcag cgtggtgctg gtctggtcag cagcaaccgc
      661 cagaatgcca acggcagcac cgtcggtggt gccatcccac gcaaccagct tacgggtggc
      721 ggtgtccagc atcagcgggg tcattgcagg cgctttcgca ctcaatccgc cgggcgcggt
      781 tgcggtatga gccgggtcac tgttgcccag cggctggtaa tgggtaaagg tttctttgct
      841 cgtcat
//
LOCUS       DAHBHC010000334          825 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHBHC010000334 DAHBHC010000000
VERSION     DAHBHC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.339"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..482
                     /locus_tag="M2A35_005555"
     CDS             <1..482
                     /locus_tag="M2A35_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCB7201672.1"
                     /translation="LSDGWYQVRIAGRDAGETELSSRLNEPLANGAVIHIVPRLAGAK
                     SGGVFQAVLGAALIATAIWMPGISIAFSDILFSMGAAMTLGGVAQMLAPKASTATTTS
                     TDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVISQEISTADEGDGGEIVVIGR"
     gene            543..>825
                     /locus_tag="M2A35_005556"
     CDS             543..>825
                     /locus_tag="M2A35_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201673.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGTEV"
BASE COUNT          188 a          180 c          281 g          176 t
ORIGIN      
        1 aactgagcga cggctggtat caggtacgga ttgccgggcg tgatgcaggc gaaaccgaat
       61 tatcatcccg tcttaatgag ccgctggcaa atggtgcagt gatccacatc gtgccgcgtc
      121 ttgcgggagc aaaaagtggc ggtgtgtttc aggcggtgct gggtgcggcg ttgattgcta
      181 cggcaatctg gatgccgggg atcagtatcg ctttcagtga cattctcttt tcaatggggg
      241 cggcgatgac gcttggcggt gttgcacaga tgctggcacc gaaagcgagt accgcaacaa
      301 cgaccagcac ggataacggt aagcagaaca cgtatttctc atcactggat aacatggttg
      361 cccagggcaa tgttctgcct gttctgtacg gtgaaatgcg tgtggggtca cgggtaattt
      421 cgcaggaaat cagcacggca gacgaagggg atggtggtga aatcgtggtg attggtcgct
      481 gatgcagaat gttttatgtg aaaccgcctc cgggcggttt tgtcgtttat ggagcgtgag
      541 gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggac aacctgaagt
      601 ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg gccgatagaa ggtccggtgg
      661 atggattaaa aagcgtgctg ctgaacagta cgccggtgct ggacagtgag gagaatacca
      721 atatctccgg cgtcacggtg gtgttccggt caggtgagca ggagcagaca ccgccggagg
      781 ggtttgaatc ctccggctcc gagacggtgc tgggtacgga agtga
//
LOCUS       DAHBHC010000335          818 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHBHC010000335 DAHBHC010000000
VERSION     DAHBHC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.340"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..403)
                     /locus_tag="M2A35_005557"
     CDS             complement(<1..403)
                     /locus_tag="M2A35_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCB7201674.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGESYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPSGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYALFRDAYHLAGT"
     gene            complement(408..>818)
                     /locus_tag="M2A35_005558"
     CDS             complement(408..>818)
                     /locus_tag="M2A35_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCB7201675.1"
                     /translation="SLVGGTVVRRKVYARFLDAVNFVSGNSNADPEQEVISRWRIEQC
                     SELSAVSASFVLATPTETDGAVFPGRTMLANTCTWTYRGDECGYNGPAVADEYDQPTS
                     DITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          175 a          274 c          211 g          158 t
ORIGIN      
        1 cagtccccgc cagatgataa gcgtcccgga acagcgcgta acagtccgtc accccgtgct
       61 caaagcgccg cccggtgaga tgcggcacac agcggaactt atgaatcgcc ccccggcaga
      121 ccagccacca cggcaaatca ctctgcacct gcagccgccg gtcagcctca ctcagccagg
      181 gcagaccact ggggtggctg tggaccagcg ccacaatctc accctgcatt tctgcctgca
      241 gccagtcctc cggagccatc cggaaataat cctccggctc accggaaata ttcacgcagg
      301 gaaaatagct ttccccctcc ggcgttctca ccacgaagcc gcacgactcc gctggcgcac
      361 atcgccgggc gtgcgccaga atcgctgatt ctgtctctgt catgggatta ctgcgaaagt
      421 ttgttaatgg aaaggaaacc gccaaagttg ccgacgttat tgcggaactt acagccgctc
      481 aggcatttgc tgcatttatc cttcgttata tcggacgtcg gctggtcata ttcatccgcg
      541 acagccggac cgttataacc gcactcatca ccgcgatagg tccaggtgca ggtgttggcc
      601 agcatggtac gtcccggaaa aacagcgcca tccgtttccg tcggcgtggc cagtacaaaa
      661 gaggcactga ctgcgctcag ttcgctgcac tgctcgatgc gccagcggct gatcacctcc
      721 tgctccggat cggcgttgct gtttccgctg acgaagttca ccgcatccag aaaacgggcg
      781 taaaccttac gccggactac cgttccgccg accagact
//
LOCUS       DAHBHC010000336          817 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHBHC010000336 DAHBHC010000000
VERSION     DAHBHC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.341"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>817
                     /locus_tag="M2A35_005559"
     CDS             <1..>817
                     /locus_tag="M2A35_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCB7201676.1"
                     /translation="TVVVNTVASENPDEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTI
                     TVYEDSQPGTLNDFLGAMTEDDARPEALRRFELMVEEVARNAEEAKKNAGEAETSARN
                     AGISASQAEKSAAHADTSAGYASESARQAAESAASAKQSEEASSSSASEAAQKASESS
                     QSAAEAELSRKTAESAAGNAARDATTATEKARESAESAQSAEQSRIAAEEAVNRIPTV
                     VGPPGPKGEQGPAGPQGPKGDKGERGDTGPVGATGERGPRGDTGPAGPQGPKGD"
BASE COUNT          213 a          208 c          283 g          113 t
ORIGIN      
        1 cacggtggtg gtgaacacgg tggcctcaga aaatccggat gaagccgggc gttacagcat
       61 ggatgttgag tatggccagt acagcgtcac cctgctggtt gaaggttttc ctccttcgca
      121 tgccgggacc atcacagtgt atgaagattc tcaacccggt acgctgaatg attttctcgg
      181 tgccatgacg gaggatgatg cccgtccgga ggcactgcga cgttttgaac tgatggtgga
      241 agaggtggcg cgtaacgctg aggaggcgaa gaagaatgcc ggagaggcgg agacgtcagc
      301 aaggaatgcc ggcatatcag ccagtcaggc agaaaagagc gctgcacatg ctgacacttc
      361 agcaggatat gcatcggagt cagcccggca ggcggcagaa agtgcagcct cagcaaagca
      421 gtcagaggag gcgtcctcgt cctcggcctc tgaggccgct caaaaagcca gtgagtcatc
      481 acaaagtgca gcagaagctg aattgtcaag aaagacggca gaaagtgcag ccggtaatgc
      541 agccagggat gcaacgaccg caacagaaaa agcccgggag tcagcagaaa gcgcacagtc
      601 agcggaacaa agcaggatag cggcggaaga agccgtaaac cgaatcccca ccgtggtggg
      661 gccacccggg ccaaaggggg aacaggggcc cgccggccct caggggccga agggggataa
      721 gggagagcgt ggtgacaccg gccctgtcgg ggcaaccggc gaacgggggc cgagaggaga
      781 tactggtccg gcaggcccgc aggggccgaa aggcgac
//
LOCUS       DAHBHC010000337          806 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHBHC010000337 DAHBHC010000000
VERSION     DAHBHC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.342"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..72
                     /locus_tag="M2A35_005560"
     CDS             <1..72
                     /locus_tag="M2A35_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCB7201677.1"
                     /translation="DMELYQTFTDEYFEPAFAVLGGA"
     gene            72..584
                     /locus_tag="M2A35_005561"
     CDS             72..584
                     /locus_tag="M2A35_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCB7201678.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            639..>806
                     /locus_tag="M2A35_005562"
     CDS             639..>806
                     /locus_tag="M2A35_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201679.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRD"
BASE COUNT          203 a          182 c          241 g          180 t
ORIGIN      
        1 gacatggagc tttaccagac gtttactgat gaatactttg aacccgcatt tgcggtgctg
       61 ggaggtgcct gatggctgtg ccaaaacatc ttcgcttttt tacgctgttt gtggatggtg
      121 aaaacgaagt gggtaaggtg acgtccgtca ctctgcccaa actgacgcgc aaaaccgaca
      181 gctaccgggg gggcggcatg atgggtgcgg taagtattga tctcggtctg gacgactccg
      241 cgcttgatgc gagctttgtc atggggggcg cggttcgtga gctgttcctt aagtatggcg
      301 gcacgattga cggcacgctg ctgcgttttg cgggtgaata ctacaccgat gcagaaagcg
      361 acctgtatga agtcgaaatg cgcggacgtg tgacggaaat tgatatgggg gaagccaaac
      421 agggcgaagc cacatcacac acttacgcca ttaaaaacac ctactacaag ctgagtgtta
      481 acgatcgccc gttgtgggag attgacctgc tgaacttcat ttaccggaag gacggcaagg
      541 acattgtgcc cgatcgcatc cgttccgcgc tcgggcttgg ctgataagta atatgcaggc
      601 ggcgcagtgc gtcgcctctg actgaaagga gttttctgat gaaagagacg aaaaacatcg
      661 ataccgaaaa cacggtcgtt gctgacactg tgaaagaaac cagtgagcgt ggcgtaaaac
      721 ttacccaacc aattgagcga ggcggcgaaa aaatcacgta tgtggagatc actggagcta
      781 ttgagcaggc tggatctctg cgagat
//
LOCUS       DAHBHC010000338          801 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.343, whole genome shotgun sequence.
ACCESSION   DAHBHC010000338 DAHBHC010000000
VERSION     DAHBHC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.343"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..131)
                     /locus_tag="M2A35_005563"
     CDS             complement(<1..131)
                     /locus_tag="M2A35_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCB7201680.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSA"
     gene            complement(174..410)
                     /locus_tag="M2A35_005564"
     CDS             complement(174..410)
                     /locus_tag="M2A35_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCB7201681.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          250 a          159 c          132 g          260 t
ORIGIN      
        1 tcagccgatt tcattacgag taattgctta ataagagtct gcaatgaaaa atttatcttc
       61 atgcgcagta tctggatcac cgcctggcca gtaatttcat attttatacg ttcattatca
      121 gatgtcttca taacgacttc atagctaacc tgttaaataa tcaggctgtt gcattattca
      181 aaaagcaacc caattagtgc attataatgt ttctgttcct cttccccagt cgcgtgttcc
      241 agtcggcaaa gaagtttggc acaaagcgac tttcgactca gattacgtcc atctttcaaa
      301 atctcagtca caatttcccc gagagtttcc tgctgagtag gcaagttagc ttttgcaaag
      361 taacgagtga ttgctcccgc agaatctaaa gtcactgaat tttgatgcat gctacgcctc
      421 tgttaaaaat gttaatcaat gttaagtgag gtaacaatag caaacttgta cgaaatatgt
      481 gtacagaatg ataaattaat tttttgcaat tagtgctaat gcatataaaa caatgtgtta
      541 tgataaaaca tattgcatca tttacctgac aatctataca agatttttcg ccaactagtg
      601 ttcaactttt tccatttttt atcaatgtaa agaaacggtt atgttttggt attaaaagaa
      661 agacacaaga agatatgtct gttaccgtct tactctcgcc tcacccatta ccctggattg
      721 ttaatgctta ccacccttat ttatcgtagc catatacgtg acgacgaacc tgtcaaaaaa
      781 atcgaagaaa tggtttcgat a
//
LOCUS       DAHBHC010000339          787 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHBHC010000339 DAHBHC010000000
VERSION     DAHBHC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.344"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>787
                     /locus_tag="M2A35_005565"
     CDS             <1..>787
                     /locus_tag="M2A35_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201682.1"
                     /translation="KKAKDSITGLKEFAENIKPAKLRFDNVTKGYKKAQNEVDKIAQQ
                     KAKTEKQNQRAQAAAARAQARQQAQIAARKEKAELKLLDVSSSISAMHRLSVAEQYKA
                     IAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRKAQLAPVKGGSGNSATMGA
                     LLFGGATAAAGVMAVSKASEFMANSFANAETQGELIQRARLGGVDVNQMYNITEWAYK
                     NGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAELVKDKKTGKS"
BASE COUNT          287 a          154 c          193 g          153 t
ORIGIN      
        1 gaaaaaagcg aaagactcta ttacaggctt aaaggaattc gcagaaaata tcaagccagc
       61 taaattaagg tttgataatg tcactaaagg ctataagaaa gcacaaaacg aagtagacaa
      121 gattgctcaa cagaaagcca aaacagaaaa acagaatcaa agagcacaag ctgccgcagc
      181 aagagcacag gcaaggcaac aggcacaaat agcagcacgc aaggagaaag cagaattaaa
      241 gctccttgat gtgtcaagct ctatcagtgc aatgcaccgt ctctctgtag ctgaacaata
      301 caaagctatt gcacaggcta gagagatagc aaaacaatat gagcaagggg ctatctcgct
      361 tgcacgtatg aatagccaga tgaaacgcct acaacagcaa caacgtaaga ttaatggcaa
      421 ccgaaaagca caattagccc ccgttaaggg aggatcaggg aattccgcca ctatgggcgc
      481 tctgttattt ggaggcgcta cggctgctgc tggcgtgatg gctgttagta aagcctctga
      541 atttatggct aatagcttcg ctaacgcaga gacacaagga gagctaattc aacgtgcaag
      601 gcttggcggt gtagacgtta accagatgta taatattaca gaatgggcgt ataaaaacgg
      661 cgttgactct atgatgggtg atcaaggggc tagaaaatac ctggatcaga tgaaagatgt
      721 tagggaacgt gccgcaaaat cctacagtga agctgaattg gtgaaagata agaaaaccgg
      781 aaaatct
//
LOCUS       DAHBHC010000340          776 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHBHC010000340 DAHBHC010000000
VERSION     DAHBHC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.345"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..200)
                     /locus_tag="M2A35_005566"
     CDS             complement(<1..200)
                     /locus_tag="M2A35_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCB7201683.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLA"
     gene            complement(197..>776)
                     /locus_tag="M2A35_005567"
     CDS             complement(197..>776)
                     /locus_tag="M2A35_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCB7201684.1"
                     /translation="SGYISAAPHKTGAGILNPIQSKAHNRASGFFVRTVLPRLFRVRI
                     MAGRTGPTSVGPDSLLSGVENPVRLATPRFSTLDGELFLSPSKEATPWQTANNSAHTL
                     RVVTSRLKSTVDFSAHHASRKSCTSICCMSAATRYRTSIPPLFSAIWRMICASCNNSS
                     SSKTNSINSCSGPFLHLAAGGLRTSVVKRIAA"
BASE COUNT          179 a          185 c          232 g          180 t
ORIGIN      
        1 aacgccagcc cgtgtggtgt gaagtccacg cggtagcagt cgtctactgt gaaataaagg
       61 gtgtctgcat tctccggttt tgtggtgcgt gctctgttgt cacgaccacg gatgtagaga
      121 tcaaataatc cctgaagaac gggagccaga cgggtgtcct gtgtgcgcac ccatcttgtg
      181 aagtcatgag cgtcaatcat gctgcaattc tctttactac agatgtgcga aggcctcccg
      241 ccgcaaggtg caggaaaggc ccggaacagg aattaatgga gtttgttttg ctgctggaag
      301 agttgttgca gctcgcgcag atcatccgcc agatagctga aaacagaggc ggaatagatg
      361 ttcgatagcg cgtggctgcg ctcatgcagc atattgatgt gcatgatttg cgcgacgcgt
      421 gatgcgcgga aaagtctacg gttgatttca gtctggatgt gacgacgcgc agcgtatgcg
      481 cgctgttgtt tgcggtttgc catggtgtgg cctctttgct cggtgataga aatagctcac
      541 catccagagt tgagaatctc ggggtggcga gacgtacagg gttctcaaca ccggagagca
      601 aagaatccgg cccgaccgaa gtcggccccg tacgccccgc cataattctg acgcgaaaaa
      661 gacgtggcaa tacagtacgc acaaaaaaac cgctggcgcg gttatgcgct ttgctctgta
      721 tcgggttgag aatcccggca cccgttttat gaggtgcagc ggaaatgtaa cctgac
//
LOCUS       DAHBHC010000341          768 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHBHC010000341 DAHBHC010000000
VERSION     DAHBHC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.346"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..278)
                     /locus_tag="M2A35_005568"
     CDS             complement(<1..278)
                     /locus_tag="M2A35_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCB7201685.1"
                     /translation="MSKVFICAAIPGEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFV"
     gene            complement(372..563)
                     /locus_tag="M2A35_005569"
     CDS             complement(372..563)
                     /locus_tag="M2A35_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCB7201686.1"
                     /translation="MNPVFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(560..748)
                     /locus_tag="M2A35_005570"
     CDS             complement(560..748)
                     /locus_tag="M2A35_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCB7201687.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEEAINKRKHERELLNKV
                     CIVSMLARLRLMPKGFAQ"
BASE COUNT          214 a          175 c          159 g          220 t
ORIGIN      
        1 acaaaagaag cagactcctc atcccagcgg ttttcctgca tatattcagc atcccatgaa
       61 tcgagggcag ggcggggtat gccaggttta tcctcgcaga caataaattt ataagcgcag
      121 tcctgagcag ccggataatg ttccaggaat tgccagtgaa attttgcgcg ggcgcgacgt
      181 tcatcaccgg cttcaatggc agtggctaca gcgacggcac cttcttcctt tattgcctgt
      241 tcgcccggaa tggcggcgca aataaagact ttactcattt tgttttaacc tcatgacaga
      301 tttaaggatg aacaaatccc tgccattgct ggcatataag aatgaaaccg gatatttatt
      361 acggaactgt tttaaagacc tgccgggatt tcgatattat cctggtgaat aactttatcg
      421 accgggtaac agttaccggg aattttctgt tcggttgctg ctgccataca ttcctgcatt
      481 gttctgtgaa cactgactgc aatatcaact ggctctccgg aaacaagaaa aaccgtcaga
      541 ataagtgcaa atactggatt cattgtgcaa atccttttgg catcagacgt aaacgggcca
      601 gcattgaaac aatgcatact ttatttaata actcccgttc gtgttttctt ttgttaatgg
      661 cctcttcagt aaatacagga ttactgatag tgacaccaat ttcaaaacaa ccttcagacg
      721 tattaacgtt tggtaataac gttttcatta tcgcgccctc aacaatga
//
LOCUS       DAHBHC010000342          757 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHBHC010000342 DAHBHC010000000
VERSION     DAHBHC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.347"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..113)
                     /locus_tag="M2A35_005571"
     CDS             complement(<1..113)
                     /locus_tag="M2A35_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201688.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKA"
     gene            complement(113..721)
                     /locus_tag="M2A35_005572"
     CDS             complement(113..721)
                     /locus_tag="M2A35_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCB7201689.1"
                     /translation="MTRPLIDVAAATRGLITAFDIDSAVGVQLDILGLWIGRSRVVSQ
                     PISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYLSDETYRVILKAKIAINNWDGRNDS
                     LPAILDAATAGSGLRMQIVDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAG
                     VWAGGIETPSVETPSEGSKFFGLDMDNEFISGFDVGAWGVLL"
BASE COUNT          205 a          212 c          151 g          189 t
ORIGIN      
        1 gctttccctg ctgtaaatcc ggatataagc gcaggcaacg cttcccattc ctcctgcgac
       61 ataacattgg catttcgatc agttgcaaac gctttaaagt cattttttgc catcagagta
      121 atactcccca tgcccctaca tcaaaaccac tgatgaattc gttatccata tcaagaccaa
      181 aaaatttaga gccttccgat ggagtttcca ccgaaggtgt ttcaatgccc cccgcccata
      241 ccccggcggc ttttactgtt agatacccct gtttaattgc cgcaattaac tcacgcgata
      301 catctgaaat atcagtatca ggaaagaccc agaccgatat cgtcatgtcc tggttatcga
      361 ctatctgcat tcgcagcccg gatcctgctg ttgcagcgtc aagaattgct ggaagcgaat
      421 cattccgtcc gtcccagtta ttaatcgcaa tcttcgcttt aaggatgaca cgataagttt
      481 catcgctgag gtacatgtat ccggaatcag gatcgtatgg cccctgccat acaccctgat
      541 catatccaag cccgtcggta tcccagctga aatagacacc tgaaataggc tggctgacaa
      601 cacggctacg tccgatccac aatcccagaa tgtcaagttg cacaccaacc gcagagtcaa
      661 tatcaaatgc agtaatcagc cctctggtgg ccgccgcaac atcaataagc ggccgggtca
      721 tcagatcaac atgtgcaaga aatttaggtt tggtggc
//
LOCUS       DAHBHC010000343          754 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHBHC010000343 DAHBHC010000000
VERSION     DAHBHC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.348"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(293..>754)
                     /locus_tag="M2A35_005573"
     CDS             complement(293..>754)
                     /locus_tag="M2A35_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCB7201690.1"
                     /translation="GVKFSHRGSKMRTPHLVPLSKQAVAILAELQTWAGENGLIFTGA
                     HDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQ
                     ERNGVRAAYIHKAEHLEERRLMLQWWADFLNANREKCISPFDYAKINNPLK"
BASE COUNT          177 a          191 c          167 g          219 t
ORIGIN      
        1 ccagtcctgc atatgatctg tgacgtaatg cagcaatttt tctgactccg cattgttcag
       61 acggcaggtg ccgatcagtg agcacaatat gtgctgcata tttgccgccg ctgtcggaac
      121 ctgcagcata ttacaggtta ttactcgatc ccgatggatg atattggggg atttctttcc
      181 atgaaaatac gtaagagtcg ggagtaattt gtcttaccat caatcttctt catcacgccc
      241 aaagaaaaat agtcattcaa agtaccccgg gcattttgcc cggggattat tactatttta
      301 atggattgtt gatctttgca taatcaaacg gactgataca tttctctctg ttcgcattca
      361 gaaaatctgc ccaccactgt agcatcagcc ggcgttcttc cagatgttcg gctttatgga
      421 tatacgcagc gcgtacaccg ttacgttcct gatggctcat ctgacgttca acagcatcgc
      481 gggaccacaa acctgattcg atcagcgcac tacatgccat cgcacggaat ccatggccac
      541 agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca gtattttcac
      601 tgattggttt acgcgggtca tgtgctcccg taaagatcag accattttca ccagcccatg
      661 tctgtagttc tgccagtatg gctacagcct gtttgctgag tggcacaaga tgtggtgttc
      721 gcatcttgga gccccggtgt gagaatttta cgcc
//
LOCUS       DAHBHC010000344          752 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHBHC010000344 DAHBHC010000000
VERSION     DAHBHC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.349"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>752
                     /locus_tag="M2A35_005574"
     CDS             <1..>752
                     /locus_tag="M2A35_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201691.1"
                     /translation="LTNENGSHTEFSWDVLDRLIQQRGFDGRTQRYRYDLTRKLTQSE
                     DEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRD
                     YGWNDNGDLVRISGPRQTREYGYSATGRLESVR"
BASE COUNT          177 a          219 c          254 g          102 t
ORIGIN      
        1 ctgaccaacg agaacggtag ccatacagaa tttagctggg atgtgcttga ccgcctgata
       61 cagcagcgcg gctttgacgg gcggacgcag cgttaccgtt atgacctgac cagaaaactc
      121 acgcagagtg aagacgaggg gcttatcacc ctctggcact acgatgcgtc ggaccgcatc
      181 acgcaccgga cggtgaacgg cgacccggca gagcagtggc agtatgatga gcacgggtgg
      241 ttaaccaccc tcagccatac cagtgaaggc caccgcgtgt cggtccacta cggctacgac
      301 gataagggcc gcctgacggg cgaacggcag acggtggaga acccggagac gggggaactg
      361 ctgtggcagc atgagacgaa acacgcctac aacgaacagg ggctggcaaa ccgcgtcacg
      421 ccggacagcc tgccgccggt ggagtggctg acctacggca gcggttacct ggcgggcatg
      481 aagctgggcg ggacgccgct ggtcgagtat acgcgggaca ggctgcaccg tgagacggtg
      541 cgcagcttcg gcagcagggc aggcagtaat gccgcatacg aactgaccag cacatacacc
      601 cccgcaggcc agttacagag ccagcacctg aacagcctgg tatatgaccg tgactacggg
      661 tggaatgaca acggcgacct ggtgcgcatc agcggtccgc gacagacgcg ggaatacggc
      721 tacagcgcca cgggcaggct ggagagcgtg cg
//
LOCUS       DAHBHC010000345          751 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHBHC010000345 DAHBHC010000000
VERSION     DAHBHC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.350"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..354)
                     /locus_tag="M2A35_005575"
     CDS             complement(<1..354)
                     /locus_tag="M2A35_005575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201692.1"
                     /translation="MKKSYIRYAIPVVSSWLFLASAYGEEGLIPPDHVTKEAEIACIG
                     LAQKYASESRRFNPDDMKKFVMEQANDCRVFLSYSIFKPNTASIILDKIESDYGRFTP
                     DVSEEEAKFRDYFHKN"
BASE COUNT          230 a          154 c          153 g          214 t
ORIGIN      
        1 gttcttgtga aaatagtctc tgaacttggc ctcttcctcg gatacatctg gagtaaaacg
       61 accatagtca gattcaatct tatctaaaat tatgcttgca gtattgggct tgaagattga
      121 gtatgacaaa aaaactcgac aatcattcgc ttgttccatg acgaattttt tcatgtcatc
      181 agggttaaat cttcttgatt cagaagcgta tttttgagcc aacccaatgc aagcaatctc
      241 agcttccttg gtgacatgat ctggtggaat aagtccttct tctccatacg ccgaagctaa
      301 gaatagccaa gaggagacaa caggaatggc gtaacgaata tatgatttct tcatattata
      361 atccttgtgc tgttttgatg attctaccca ttaaatgaag aaaaaacacg caatgcctac
      421 tcaggtgggc ttttttaagt tattaccaat aggttattat catgaaagca attgaacact
      481 ctcacaaaga gatgatgcac gcacgattgg ttgcgagtgg gtatacccca gaagaagcag
      541 acaaactcgt aaaccatgat gaaacagact tagccgccgt gataaagcta aatttggcat
      601 attctctatc cctgtgtagt ttagagagca cactcaggtc cattgttgcg tcacctaaat
      661 caacctcggc agacgcggta gaagcgatga agttgctagt agagctatca gcacggcatg
      721 agcacttatt caagtcctaa cttgtcatgg a
//
LOCUS       DAHBHC010000346          744 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.351, whole genome shotgun sequence.
ACCESSION   DAHBHC010000346 DAHBHC010000000
VERSION     DAHBHC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.351"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..488
                     /locus_tag="M2A35_005576"
     CDS             <1..488
                     /locus_tag="M2A35_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCB7201693.1"
                     /translation="LLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVV
                     VFNNVIARFRRTSWSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEI
                     IVTTFTAGHERTLPLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNG
                     Q"
     gene            510..>744
                     /locus_tag="M2A35_005577"
     CDS             510..>744
                     /locus_tag="M2A35_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCB7201694.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENG"
BASE COUNT          160 a          173 c          221 g          190 t
ORIGIN      
        1 ttttactgct gctgcccatt gcgctgccgg gcattgtcac tggtctggcg ctattaaccg
       61 cttttaaaac catcaatctg gagccgggat ttttcaccat cgtggtcggt catgcgactt
      121 tttgcgtagt ggtggtgttt aacaatgtca tcgcccgttt tcgccgcacc tcgtggagtc
      181 tggttgaggc gtcaatggat cttggggcca atggctggca aaccttccgc tacgtagtgt
      241 tgccgaatct cagttcggcg ctactggcag gaggaatgct ggcgtttgcc ttgtcgttcg
      301 atgaaatcat cgttacgact tttacggcag gtcatgaacg aacgttaccg ttgtggttgc
      361 tcaatcagct tgggcgaccg cgtgatgtac cggtaactaa cgtggtggca ctgctggtta
      421 tgttggtaac aaccttgccg atcctggggg cctggtggct aacccgcgaa ggcgacaatg
      481 gtcaataacc actgatacag gaatatgcta tgcaacataa gttactgatt aacggagaac
      541 tggttagcgg cgaaggggaa aaacagcctg tctataatcc ggcaacgggg gacgttttac
      601 tggagattgc cgaggcatcc gcagagcagg tcgatgctgc tgtgcgcgcg gcagatgcag
      661 catttgccga atgggggcaa accacgccga aagtgcgtgc ggaatgtctg ctgaaactgg
      721 ctgatgttat cgaagaaaat ggtc
//
LOCUS       DAHBHC010000347          742 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHBHC010000347 DAHBHC010000000
VERSION     DAHBHC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.352"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(8..>742)
                     /locus_tag="M2A35_005578"
     CDS             complement(8..>742)
                     /locus_tag="M2A35_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCB7201695.1"
                     /translation="QCEQVNYDTFISYPQLDAWAAHPDFQSRVSTQIARQVALDRIMI
                     GFNGTSHADESNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGK
                     YANADALVQDARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAG
                     QLILSSRAIGGLDVFLAPFFPDSTMLITSFNNLSIYWQKGTMRRLMKDEPEYNRIATY
                     QSINDAYVVEDYGKCAMVTGLKFADS"
BASE COUNT          156 a          197 c          215 g          174 t
ORIGIN      
        1 gagttgatta gctgtcggcg aacttcaggc cagtgaccat cgcgcacttg ccatagtctt
       61 caacgacata agcgtcattg atggactggt aggtggcgat gcggttgtat tccggctcgt
      121 ctttcatcag gcgacgcatt gttcctttct gccagtaaat tgacaggttg ttgaacgagg
      181 tgataagcat cgttgaatcc gggaagaacg gcgcaaggaa tacatccagc ccgccaatgg
      241 cgcgcgatga caggatgagc tgtccggcaa gtaattccgc attgggattc tggccgctga
      301 tgctgttcag cacgggcaga cgcagcgagt taaacaggtt gcgccccata atcaccacga
      361 ggtcgtcagc ttccttgtgc cattcatcca gcagggatga gcgcgcgtcc tgtaccagag
      421 catcagcgtt cgcatactta cccgcgtgcg ccacggtgtt gtccatgttg cgggaggtca
      481 gcgtcacgtc attcattacg cgctcgctgg cgtcggttct gatgtgctcc agccatccca
      541 cgttaacgtc ctgaagcagc ttgttagtgc tgaagttgga ctcatctgcg tgagacgtgc
      601 cgttgaaacc gatcatgatg cggtcaagcg ccacctgccg ggcaatctgt gtgctgacgc
      661 gggactgaaa atcagggtgt gccgcccagg catcaagctg cggatacgaa ataaacgtgt
      721 cgtagttcac ctgttcgcac tg
//
LOCUS       DAHBHC010000348          740 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHBHC010000348 DAHBHC010000000
VERSION     DAHBHC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.353"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>740
                     /locus_tag="M2A35_005579"
     CDS             <1..>740
                     /locus_tag="M2A35_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7201696.1"
                     /translation="KSRNSLLFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKH
                     RDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGD
                     KRIEGSVWPKSIRGSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDEST
                     PFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGD
                     EIPPPRSITFHIWTAYSPFTTWVQIVYD"
BASE COUNT          164 a          192 c          208 g          176 t
ORIGIN      
        1 taaatcccga aacagtctgc tttttcagcc cacggattct gccgctgaag attttatgaa
       61 gtctcacgtg gaggcgacga ttcggaacgt gccatgcctg aaagaccttt ccccatggct
      121 gggtcgtaaa catcgtgaca atactctcac gctgaaacgc ttttcatcgg gcgtcggttt
      181 ctggtgcctg ggcggcgctg ccgccaaaaa ctaccgtgaa aaatccgtgg acgtggtctg
      241 ctatgacgaa ctttcctcgt tcgagccgga tgtcgaaaaa gagggctcgc caaccctgct
      301 gggggataag cgtattgagg ggtcggtgtg gccaaaatcc attcgcggct cgacgcctaa
      361 aatcaaaggc acctgccaga tcgaaaaagc cgctaacgag tcggcgcatt ttatgcgttt
      421 ttatgtgccc tgcccgcact gtggggagga gcagtatctg aaatttggcg atgagtccac
      481 gccttttggg cttaaatggg agaaggacag ccctgaaagt gttttctacc tctgtgagca
      541 tcatggctgt gtgatccatc agtctgaact ggaccagagc aacgggcggt ggatctgtga
      601 aaacacgggc atgtggaccc gtgacggtct gacgtttttc agcgcccggg gtgatgaaat
      661 tccgccgccg cgctccatca cgttccatat ctggacggcg tacagtccgt tcaccacctg
      721 ggtacagatt gtctatgact
//
LOCUS       DAHBHC010000349          731 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHBHC010000349 DAHBHC010000000
VERSION     DAHBHC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.354"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>731
                     /locus_tag="M2A35_005580"
     CDS             <1..>731
                     /locus_tag="M2A35_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HCB7201697.1"
                     /translation="YEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQL
                     VKKNQTRITGMDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVE
                     WQNRPLDAVYPIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGA
                     KFWLNVLTELKNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVS
                     WKDYKAVTRDLKAIYQAPTEEAGQQA"
BASE COUNT          199 a          186 c          191 g          155 t
ORIGIN      
        1 tatgagaaaa atcagtccag accaggagct aactcccgca acggttattc cacaaagacc
       61 gttatcacag gcgacggtcc actggaactg cgtactccgc gcgatcgtga cggtaccttc
      121 gaaccacaac tggtaaagaa aaatcagacc cgtattaccg ggatggataa ccagatcctc
      181 tcgttgtatg ccaaagggat gaccacccgt gagatagcta ctgcgttcaa agaactgtat
      241 gacgcagatg tttcaccggc actgatatca aaggtcaccg atgccgtgat ggagcaggtt
      301 gtagaatggc aaaacagacc actggatgct gtttacccca ttgtttatct tgactgtatc
      361 gtcctgaaag ttcggcagga cagccgcgtc atcaacaaat cggtgttcct ggcactgggc
      421 atcaatatcg aaggtcagaa agaactgctg ggtatgtggc tggccgaaaa tgaaggggcg
      481 aagttctggc tcaatgtgct gactgaactg aaaaaccgcg gtctgaacga tatcctcatc
      541 gcctgtgtgg atggcctgaa aggcttcccg gatgccatca acacagtata tccgaaggcc
      601 cgcatccagt tatgcatcgt gcatatggtg cgcaacagcc tgcgcttcgt gtcatggaag
      661 gactacaaag ccgtcactcg cgacctgaaa gcgatttatc aggctcccac ggaagaggca
      721 ggtcagcagg c
//
LOCUS       DAHBHC010000350          725 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.355, whole genome shotgun sequence.
ACCESSION   DAHBHC010000350 DAHBHC010000000
VERSION     DAHBHC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.355"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            14..298
                     /locus_tag="M2A35_005581"
                     /pseudo
     CDS             14..298
                     /locus_tag="M2A35_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(308..>725)
                     /locus_tag="M2A35_005582"
     CDS             complement(308..>725)
                     /locus_tag="M2A35_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCB7201698.1"
                     /translation="YGSGEPLTIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLL
                     AYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGHIPELMRDLASM
                     GQLVKLPTIRERAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT          154 a          206 c          163 g          202 t
ORIGIN      
        1 tggacactat cgtatggaat accggacctg gatcactgaa gctttacgtc ttcactttga
       61 agaacattta ccacggattg tggccggacg tcgcctgggc gtaccaaaat caacagtttg
      121 tggtatgttc gtgcgcttcc gaaaagctgg cctttcatgg cctctgcccg caggtatgtc
      181 ggagcgggaa cttgatgccc gtctttacgg gagtgcctcc acagtacctg tcgtacttac
      241 tgaaaacacc gttatgtccg aagtcccggt ggtaaaaaaa cgcccccggc ggcctgatgc
      301 cagtcagtta agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct
      361 cctttaccac tctcgggaag gccctttccc ttattgtcgg taatttcaca agttgtccca
      421 tacttgcaag atcgcgcatc agctccggta tatgtcccgg tgaagcgccc tgcaatgtca
      481 tcagcattct catcaccatt ccgcatgatt ctgagaaact cagttgattc ggccagtaac
      541 ctttcagatg ttccgccatt ttaatcatct gatatctcac cagattataa gccagtaaga
      601 caccccacag ctcttgctcc acaagctccg gctttttact tctcagcgtc agcctgctca
      661 gttgcatcgt ctgttttatc tccctgtatc ccagttcgat tgtaagcggc tcgccagaac
      721 cgtat
//
LOCUS       DAHBHC010000351          723 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHBHC010000351 DAHBHC010000000
VERSION     DAHBHC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.356"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..186
                     /locus_tag="M2A35_005583"
     CDS             <1..186
                     /locus_tag="M2A35_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201699.1"
                     /translation="QSSNIANATGDEVVSLDKHINTSATDTDQIQAFIVSTWMAPFQN
                     DMYSEDNPISPYYKIEW"
     gene            269..>723
                     /locus_tag="M2A35_005584"
     CDS             269..>723
                     /locus_tag="M2A35_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCB7201700.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQINQMHIPLLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNQKKRQQYAPKYIL"
BASE COUNT          276 a          124 c          118 g          205 t
ORIGIN      
        1 caaagttcaa atatagcgaa tgcaacgggt gatgaagtcg tatcattaga taaacacatc
       61 aacaccagtg ctactgatac agatcaaata caagcattta tagtttcaac atggatggcg
      121 ccttttcaaa atgatatgta ttcagaagat aatcctatct caccttacta taaaattgaa
      181 tggtagacga ttgaccaaat aaatcataac gagatacttt tataatagta tctcgtccct
      241 ttcattcaat cctcacacat gaaaaaatat gcgctcacca aaagttaaat tcttaaccat
      301 cttcacgttt tgtatattta ttacaaaaat gtctttcgcc agcaactctt gctctaatga
      361 agcaggaaca atgttcagaa tcgaaccaaa tttaattaaa gctatcgctc ttgttgaaag
      421 caatctaaaa aaggatagca taggcaagaa cagagataag aataacaata taaagagttt
      481 agattattgg ttaatgcaga taaaccagat gcatattccc ctgttaaaaa aacgtggaat
      541 aataaaagat gaacgagatt tgttagataa cccttgtctg aacattaaaa taggaaccga
      601 gattttatat aaccattttt cacgttgtgg agtaacgtgg caatgcctgg ggacttataa
      661 tgcgggtttt gcaatggata accaaaaaaa gagacagcaa tatgccccaa aatatatatt
      721 gta
//
LOCUS       DAHBHC010000352          720 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHBHC010000352 DAHBHC010000000
VERSION     DAHBHC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.357"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..60
                     /locus_tag="M2A35_005585"
     CDS             <1..60
                     /locus_tag="M2A35_005585"
                     /inference="COORDINATES: protein motif:HMM:NF027276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HCB7201701.1"
                     /translation="ICAAECGEHIEPYKLGNIA"
     gene            57..170
                     /locus_tag="M2A35_005586"
     CDS             57..170
                     /locus_tag="M2A35_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201702.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTGE"
     gene            263..679
                     /locus_tag="M2A35_005587"
     CDS             263..679
                     /locus_tag="M2A35_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201703.1"
                     /translation="MNTKEEGIINTLKKISEAEDEMAKDAVKRSQHMAALHALTIAKI
                     TADAAKIIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMPQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
BASE COUNT          249 a          152 c          151 g          168 t
ORIGIN      
        1 atctgtgccg cagaatgcgg ggaacatatt gaaccgtata agctggggaa tatcgcatga
       61 gtatatttat ctcatggctt gttctgatta tttctgtggc ctgcgccatt gggattatgc
      121 gaattattca ttcagtaaaa aagattgaac gctttttcac tggcgaataa cagagcaaat
      181 aaaaccacag gttaaataag aaaatgtaaa aacaatccgc attcgcggag gtattcgcac
      241 acgtaaataa cggagatata aaatgaacac aaaagaagaa ggcattatca acacattaaa
      301 aaaaatatca gaagcggaag atgaaatggc taaagatgcc gtgaagcgta gccaacatat
      361 ggcagcactt cacgcactga ccattgcaaa aatcaccgct gacgcagcca aaattattga
      421 ggaacagagc aaagaaatcg acactcttaa aacacagtca acagttgcag ccatgaatcc
      481 gtccagcatt ggacgctgca tttatattct tggttcagca atgatgccgc aatacaccat
      541 tattgccgaa ctgcacggca aatacctgat aacgccttac cacacaaaag agtctgagct
      601 tctgacaaat ctccgcctga tagaacgctc tcaagctgta ttcattgatg acgcgcaacg
      661 tgccgtattt aacgcatagg gttactggac aaagggggcg caatggcaat taagcatttt
//
LOCUS       DAHBHC010000353          706 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.358, whole genome shotgun sequence.
ACCESSION   DAHBHC010000353 DAHBHC010000000
VERSION     DAHBHC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.358"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..546
                     /locus_tag="M2A35_005588"
     CDS             <1..546
                     /locus_tag="M2A35_005588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201704.1"
                     /translation="KLPALTINLPERCYTSLSGTTITFKSDIDASTVTADGGAAVDSR
                     SITLNATCNTVLLSKSVYASVKLVQQSGVDDGYKFKLNPKKNEYNLNRYLSVVAKHVS
                     SGAAGGTNCGKDTNTFEDGKLYNVGQVAFPGRPEQSVRDPYNITFNLCAFRPEGTSDL
                     LPPGEHTGAIRVITRFYAADE"
BASE COUNT          210 a          141 c          158 g          197 t
ORIGIN      
        1 aaactgcctg ctttaaccat taatctgcct gagcgctgtt ataccagttt gtcaggaacc
       61 acaattacat ttaaaagtga cattgatgca tcaacagtta cggctgacgg gggagcggca
      121 gttgattcca ggtcgattac tctgaatgca acgtgtaata ccgttttact cagcaagagt
      181 gtttatgcat cagttaaact ggtgcagcag agtggtgttg atgatggtta taaatttaaa
      241 ttaaatccca aaaaaaatga atataactta aaccgttacc tgagtgttgt tgcaaagcac
      301 gtatcttcag gagctgcagg tggtacaaat tgcggtaagg atacaaatac ttttgaggat
      361 ggtaagcttt acaatgttgg tcaggttgct tttcccgggc ggccagaaca gtcagtcaga
      421 gacccttata atattacatt caatctgtgc gccttcaggc cagaggggac ttctgattta
      481 ctccctccgg gagaacacac cggagccatc agggttataa cacgctttta cgcggcagat
      541 gagtaacggc attatgccag cattaaaatg ataagatgaa tcagtatgat aacatcatgc
      601 tggctgtaat ttcactacat tattttttat gtgcagataa tacagttact tttccgggaa
      661 acactctgaa ctgcagcccc ctcctgaaac aggaagatgt aacgga
//
LOCUS       DAHBHC010000354          700 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.359, whole genome shotgun sequence.
ACCESSION   DAHBHC010000354 DAHBHC010000000
VERSION     DAHBHC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.359"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..406
                     /gene="cesD"
                     /locus_tag="M2A35_005589"
     CDS             <1..406
                     /gene="cesD"
                     /locus_tag="M2A35_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HCB7201705.1"
                     /translation="GGTLASLSNLTQQDLNDLHSYAYTAYQSGDVITARNLFHLLTYL
                     EHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRASYYSGISYLLVGNRKMA
                     KKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
BASE COUNT          213 a          140 c          133 g          214 t
ORIGIN      
        1 tggtggtacg ttagcatcac tgtcaaattt gacgcaacaa gatctcaatg atcttcattc
       61 ttatgcctat acagcatatc aatctggtga tgttataacc gcaagaaatc tatttcatct
      121 gctaacatat ctggagcact ggaattatga ctacacccta tctctggggt tgtgtcatca
      181 gcgtttatca aatcatgaag atgcacaact gtgtttcgct cgctgtgcaa ctttagttat
      241 gcaagatccc agggcatctt attattctgg gattagctac ttattggtcg gaaatcggaa
      301 aatggccaaa aaagccttta aggcttgttt aatgtggtgt aatgaacaag aaaaatacgc
      361 tacatataaa gaaaatatta aaaaattgtt aggtaataca gattaaaaac taccatctgc
      421 cataccaatc cccaggctgt ctggggattt tctatcggaa ataataaagc tgctttggtg
      481 aagtgcgccc aacgttgagc accgaccgct ggcagattta tctacaggag tgggttactc
      541 cattaaccag gattttatgc gcctgaacca tcttctgctg tactcaatgt catttccgct
      601 tatagaataa gcagtatata tcttaactta gcatcagaaa tctgactgtt tcaatcatct
      661 atagcccaga gtgtatttgt caggaaatga ctttcaaact
//
LOCUS       DAHBHC010000355          693 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.360, whole genome shotgun sequence.
ACCESSION   DAHBHC010000355 DAHBHC010000000
VERSION     DAHBHC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.360"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..506
                     /gene="wzx"
                     /locus_tag="M2A35_005590"
     CDS             <1..506
                     /gene="wzx"
                     /locus_tag="M2A35_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S88 variant O45 family O-antigen flippase"
                     /protein_id="HCB7201706.1"
                     /translation="INENTASVTLILIQVLAPILLYKNKKWKIFNRNLIVLSIIMFTA
                     FSLFSFSLKPFTPYIFTFVFGHDYVTSANYYNILVFLLPFFAVDNLINAANLASKSYY
                     YYTIKWCLVFLLCLSIYSALKVSAITAPIPSIFIALYISLFFSILVSIYWYLTVTKKY
                     LMERAND"
     gene            499..>693
                     /locus_tag="M2A35_005591"
     CDS             499..>693
                     /locus_tag="M2A35_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCB7201707.1"
                     /translation="MISIISPVLGSSSKVETFISALLANTHGQKNILKNIELIIIDDG
                     SEVNLEDTTIAFSEKFNEREW"
BASE COUNT          210 a          125 c          102 g          256 t
ORIGIN      
        1 agattaatga gaatacagct tcagtaacct taatattgat acaggttttg gcccccatcc
       61 ttttatacaa aaacaagaag tggaaaattt tcaacagaaa tcttattgtc ttatccataa
      121 taatgtttac tgctttttcg ttattctcat tttcgttaaa gccatttaca ccatatatct
      181 ttacatttgt tttcggccat gactatgtca catcggcaaa ttattataat attctggtct
      241 ttttgttgcc attttttgct gtcgataatt tgatcaacgc agctaacctt gcatcaaaga
      301 gttactacta ttacaccatt aaatggtgtc ttgtattttt attatgtctg agcatctata
      361 gcgcattgaa agtctctgct attactgcgc cgataccaag catctttatt gcattatata
      421 tttctctttt cttttcaata ttagtttcaa tctattggta tttaactgta actaaaaaat
      481 atttaatgga acgtgcaaat gattagcatt attagccctg ttctcggtag ctcatcaaaa
      541 gtagaaactt tcatctcagc tctgctcgct aatactcatg gacaaaagaa tatactaaag
      601 aatattgaac ttatcatcat cgatgacggt tctgaggtga atttggaaga cacaactatt
      661 gccttttctg aaaaatttaa tgagcgcgaa tgg
//
LOCUS       DAHBHC010000356          681 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHBHC010000356 DAHBHC010000000
VERSION     DAHBHC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.361"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            14..385
                     /locus_tag="M2A35_005592"
     CDS             14..385
                     /locus_tag="M2A35_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201708.1"
                     /translation="MAATTLGDSLVKIDPAYTKKETIDNMVRNVRNAFSGLYGSDNQK
                     QESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRSVNLENDANLRGR
                     TIAHPYAHTLGSVDIKDPTNL"
     gene            complement(363..596)
                     /locus_tag="M2A35_005593"
     CDS             complement(363..596)
                     /locus_tag="M2A35_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201709.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT          198 a          135 c          150 g          198 t
ORIGIN      
        1 agaaatccag acaattgctg caacgactct cggtgatagt ctggtgaaaa ttgacccggc
       61 atacaccaaa aaagaaacca tcgataatat ggttcgtaat gtgcgcaatg ctttttctgg
      121 gctatatggt tctgataatc aaaagcagga aagcgatgtt aataaacggg taatttctgt
      181 ttgcgtgaat ggccatgttc tttcatcaat caaaacagaa acggcgacag tcttcgattg
      241 cctttgcatt gtacagagcc ttgttgatgc cctgtttcgt tcagtgaatt tagaaaatga
      301 tgcaaatctg cgagggcgca caatagcaca tccatatgca catactttag gctctgtgga
      361 tatcaaagat cccacaaatc tttaatgaaa tagttaacgc gaattgtact tgctctttca
      421 gttgctttca gaatacgcgt tgaaactgct ggcggtaatt tggtattcca tttattaaaa
      481 tcatgcccgg gaaagtactc ttcgaaaata cttttaactg cagactcgcc tattgaaatg
      541 ctgcttacca tgcgattttg ataaaggcat ttagcaataa gagttgattt taacattcac
      601 cctcctgagg gttggtaatt aaggagttct ccacgggtga agtggagtgc gtgcgccgga
      661 cacgggtgaa catccggcac t
//
LOCUS       DAHBHC010000357          677 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHBHC010000357 DAHBHC010000000
VERSION     DAHBHC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.362"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..74
                     /locus_tag="M2A35_005594"
     CDS             <1..74
                     /locus_tag="M2A35_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system, LEE associated
                     protein"
                     /protein_id="HCB7201710.1"
                     /translation="VFSLMNTFQQHVQIIERVRRMTS"
     gene            71..>677
                     /gene="escV"
                     /locus_tag="M2A35_005595"
     CDS             71..>677
                     /gene="escV"
                     /locus_tag="M2A35_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HCB7201711.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAG"
BASE COUNT          174 a          122 c          149 g          232 t
ORIGIN      
        1 atgtgttttc tctaatgaat acttttcagc agcatgtgca gattattgaa cgagttcgca
       61 ggatgacatc atgaataaac tcttaaatat atttaaaaaa gcagagtcat atcacgatct
      121 tattctggct ctcttcttct ttatggctgt aatgatgatg attattccat tgccaacagt
      181 tgtggtggat atcattatcg cgattaatat atcgacagcg ttgcttttat taatgctctc
      241 aatctatata aaaaatcctc ttgagctaac ttcttttcct acaatcctgt tgattacgac
      301 gttgatgcgc ctgtcgctca gtgttagtac aaccagactt attctgctgc atcacgatgc
      361 gggtgacatt atttactcct tcggtaactt tgttgttggc ggtaatattg ttgttggcct
      421 ggttattttt accattatca ccattgtaca atttatggtt attacaaaag gggctgagcg
      481 tgttgctgaa gtgagtgctc gtttttccct tgatggtatg ccaggaaaac aaatgagtat
      541 tgatggtgat atgcgcgcag gcgttattga tccgcttgag gcaaaagttc ttcgctcccg
      601 agtgcaaaag gaaagccagt tttatggttc aatggacggg gcgatgaagt ttgtaaaagg
      661 ggacgctatt gccggta
//
LOCUS       DAHBHC010000358          672 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.363, whole genome shotgun sequence.
ACCESSION   DAHBHC010000358 DAHBHC010000000
VERSION     DAHBHC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.363"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            108..560
                     /locus_tag="M2A35_005596"
     CDS             108..560
                     /locus_tag="M2A35_005596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein
                     SufA"
                     /protein_id="HCB7201712.1"
                     /translation="MIRINSIHQLPLTSGIPDEYVFLPLDKATDYLNQLNSTQRMLLE
                     SAIRDRQARGKGALFATNEGRVRKICKEAIEQNMRVKEAINIVMQPDNLARLFPGNPE
                     QRVHYRNLLIRFGRAIAQSEFIARDVINSMEQCTAPIHRTPESIVKTE"
BASE COUNT          212 a          158 c          151 g          151 t
ORIGIN      
        1 aatcaggact ggattattgc aaaagccgag cacaccattg acaacagcgg ctttaccact
       61 cagcttgagc ttgaagcaaa aatcccggaa tggatagcag aaacagaatg atccgaatta
      121 actcaataca tcaactgccc ctgacatcag gtattccgga tgagtacgta tttttacctc
      181 tggacaaagc aactgattat ctgaatcaac tcaatagcac gcagcgaatg cttctggagt
      241 cagctatccg tgaccgacag gccagaggca aaggggcgct ttttgcaacc aacgaaggaa
      301 gagtcaggaa gatttgcaag gaagccatag agcagaatat gcgagtcaaa gaagcaataa
      361 acattgttat gcaaccggac aatcttgctc gtttattccc gggcaatcct gaacaaagag
      421 tccattacag aaatctatta attcgtttcg gtagggctat tgcccagagc gagtttattg
      481 cccgcgatgt aattaattcg atggagcaat gcacagctcc catacaccga actcctgaat
      541 caatagtaaa aacagagtga gcaacttaga atagcggcag caccacgtta agggaggtcg
      601 ctatgttccg ttgtccgctt tgtggcgcat ctgcccgtat ccgcaccagt cgtccagaaa
      661 atgattcaaa ca
//
LOCUS       DAHBHC010000359          672 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.364, whole genome shotgun sequence.
ACCESSION   DAHBHC010000359 DAHBHC010000000
VERSION     DAHBHC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.364"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(31..402)
                     /gene="ler"
                     /locus_tag="M2A35_005597"
     CDS             complement(31..402)
                     /gene="ler"
                     /locus_tag="M2A35_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HCB7201713.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKRVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
BASE COUNT          208 a          132 c           94 g          238 t
ORIGIN      
        1 cagttatcgt tatcatttaa tgatttcatg ttaaatattt ttcagcggta ttatttcgtc
       61 ttcggtgtcc ttcacaagaa aatcttcttt cttcattcca ttcaacagtg cttctttaag
      121 ccagcgtggc tgtcggccaa ctcccgacca ggtctgccct tcttcattgc gataataaac
      181 tcttttcgat gagttccggc gagcaaggcc atcatcaggc acattagtat atcccagctc
      241 ttgtagggtt atattgtaat aagtaatctg cttttgaatc gactcaatta cactttgaat
      301 ttcctgctct cgcagtcgct ttgcttcctg ttgtaacact tcaatttgct caataagctg
      361 aatgtatggg cttgttgtgt gcgaattagt ttccatattc ataataaata atctccgcat
      421 gctttaatat attaagctat tagcaatctt atccttatca ggaaggatca acaattaatc
      481 gaatgatata atttatgtga gataacgttt tatctatcaa attagaacac atctacttca
      541 tcaaacaaaa tccttataaa agttattaat caccgatgta taatgtttaa aacacattat
      601 cattaaatgt caacaaaaaa atcatatttc ttaaaaatca ttcaaaatca ataaagtaga
      661 agaacttcat ct
//
LOCUS       DAHBHC010000360          670 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.365, whole genome shotgun sequence.
ACCESSION   DAHBHC010000360 DAHBHC010000000
VERSION     DAHBHC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.365"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..96
                     /locus_tag="M2A35_005598"
     CDS             <1..96
                     /locus_tag="M2A35_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCB7201714.1"
                     /translation="GCASLSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            93..>670
                     /locus_tag="M2A35_005599"
     CDS             93..>670
                     /locus_tag="M2A35_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7201715.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVASAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDHRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLAT"
BASE COUNT          176 a          198 c          179 g          117 t
ORIGIN      
        1 gggtgtgcct cgctgagtga atacaccgct gaatttaacc gcctttctgc cctcttctcg
       61 caggagtggc agcgcgtgac gggataccag ccatgagtga gtttgcatgg agctggaatg
      121 agccacggcc agccattgat ccggccagat ttacggagca caggcaggaa actgaaaccg
      181 acctgcaacg cgccatccgt tactaccttg aggcagacaa aaaggctctg gaagaacagg
      241 aagcgaagga ggaagccttt ttcgcacaat ccaccgtggg taaaaaactc atggcatccc
      301 ttgaggaagc cggacagcgt gaaaagctgg cacaaagcat catcagcaag cgtcaggcaa
      361 cagaacaaga cccggtggcc agtgcttttg ccacactgaa ggtgcttccc gtttatctgc
      421 gtgaacctct gagccgccac ctctctttcc tgcgcaaaaa acaggaagcc gatcgccaga
      481 aaggcaaaaa gagctggcag gctgaacgct acgcgcgcgg aaccctgcgc aaaatattcg
      541 aacgcctgga ccgcaccgac caccgctggc tgacaccggg ttatcgctcc cttgccggac
      601 gcgaacgcct ggatgatttg ctttacctgc cgcagctcaa caaacaccag atacagacgc
      661 tggccaccat
//
LOCUS       DAHBHC010000361          666 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.366, whole genome shotgun sequence.
ACCESSION   DAHBHC010000361 DAHBHC010000000
VERSION     DAHBHC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.366"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>666)
                     /locus_tag="M2A35_005600"
     CDS             complement(<1..>666)
                     /locus_tag="M2A35_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7201716.1"
                     /translation="HPDEIETIVSHVCDIAIQEDRHELGDDITPRFKAEYVDGSKGTP
                     TSYIATYIGKNLDSRAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQF
                     FGIPSRQVWRELRRLASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLV
                     PRKDYLIRTAYDLADELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAE
                     DSAR"
BASE COUNT          117 a          188 c          198 g          163 t
ORIGIN      
        1 gcgggcgctg tcttccgctt ccggtttacg tcttaccagc ttccagttat ccggatgtgt
       61 gcacacacgg gaagactccc cgatgagtgg tgaccagatc ccgtaaatct gtacgctctg
      121 ttcgccgtaa tcgttcagct catctgcgag gtcgtaggcg gtgcgaatca ggtagtcttt
      181 gcgtggaaca agtacgccgc cctgtttctc aatgtaggtg gcaaaacacc cggcatcagc
      241 ggcagcgagt accgcatcca ttgcatcatc cttcagccgt tgtgggcctt ccgggttgcg
      301 tgccatctgg ctggcaaggc ggcggagttc acgccatacc tgacgagagg gaatgccaaa
      361 gaactggaac tggcggaccc ggtgaaggcg cgcccagccg atggcgcgtt ccacgctctc
      421 tgccattgat tttccggttt cgtggtcaac gcgtggcttg cccgttttcg ggtcgatgcc
      481 atccacggcg cggctgtcca ggttctttcc gatgtaggtg gcgatgtagc tggttggcgt
      541 gccttttgag ccgtctacgt actcagcctt aaaacgcgga gttatgtcat cgcccagctc
      601 gtggcggtct tcctgaatgg caatatcgca gacatgggac acgatggttt caatctcgtc
      661 cggatg
//
LOCUS       DAHBHC010000362          665 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHBHC010000362 DAHBHC010000000
VERSION     DAHBHC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.367"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..273
                     /locus_tag="M2A35_005601"
     CDS             <1..273
                     /locus_tag="M2A35_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201717.1"
                     /translation="TNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAE
                     DEAPPGIGEPDDEPQEPSGGNPVSNQPTQDSAGGRGHRKWSLRWFK"
     gene            164..>665
                     /locus_tag="M2A35_005602"
     CDS             164..>665
                     /locus_tag="M2A35_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCB7201718.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGHYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADIVRKNDEQWRKHSKTISRELRNLVNSAPPGQVMKS
                     IVAEQVKYIKSLPLEAADRVYDIQNRAIEAVVTGGRAEHFAKEIAASGDIAKSRADLI
                     ARTELGR"
BASE COUNT          188 a          162 c          192 g          123 t
ORIGIN      
        1 accaacgcaa tcagtacagc gctgggtgat ggtctgatga cactgaaagc cgctatgact
       61 gatttgcgag aaaattctga cgtaaccggc atcggggcat ccattaccga cgaggacatc
      121 gagaatgccg aagatgaagc gccgcccggc atcggcgaac ctgatgacga accgcaggaa
      181 ccgtcaggcg gaaatccggt atcgaaccag cctacgcagg atagcgcggg cggtcgggga
      241 catcgtaaat ggtcactacg atggttcaaa tgacagtatc acggaaatta ttgaggcgct
      301 ggaacgctac agtgaaatca tcaccccctg ggcgacaaag gtcgcggaaa actttaccgc
      361 cgatattgtg cgcaagaatg atgagcagtg gcgtaaacac agcaaaacca tcagccgtga
      421 gctacgcaat ctggtaaaca gtgccccgcc agggcaggtg atgaaatcca tcgttgctga
      481 acaggttaag tacattaaat cgctacccct cgaggcggct gacagggtgt acgacatcca
      541 gaatcgggcg attgaagctg ttgtgaccgg tgggagagcg gaacattttg ctaaagaaat
      601 agccgcatcg ggtgatatag caaagtccag agctgacctg attgcccgta ctgaacttgg
      661 acgtg
//
LOCUS       DAHBHC010000363          665 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHBHC010000363 DAHBHC010000000
VERSION     DAHBHC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.368"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..142)
                     /locus_tag="M2A35_005603"
     CDS             complement(<1..142)
                     /locus_tag="M2A35_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCB7201719.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHD"
     gene            complement(142..>665)
                     /locus_tag="M2A35_005604"
     CDS             complement(142..>665)
                     /locus_tag="M2A35_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCB7201720.1"
                     /translation="HQIKIGEWIHADFKRVRNYAFHKRFFKLLQLGFDYWTPVGGAIT
                     PRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDTALLKSFDAFREWV
                     TIQAGFYTEHIYPDGSRGRRAKSIAFANMDETVFQQVYKSVLNVLWNWILFRKFSSPE
                     EVENVAAQLLEFA"
BASE COUNT          161 a          182 c          156 g          166 t
ORIGIN      
        1 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
       61 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
      121 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
      181 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacaacacat tcagtacaga
      241 tttataaacc tgctgaaaca cggtttcgtc catgttcgca aatgcgatag attttgccct
      301 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
      361 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
      421 aacggtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
      481 tgattcgcac aggtaatcaa cgaagcccga taccagtttt cgttcgcgag gcgtgatcgc
      541 cccaccgacc ggagtccagt aatcgaatcc cagttgcagg agtttgaaaa aacgcttgtg
      601 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
      661 atgca
//
LOCUS       DAHBHC010000364          662 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.369, whole genome shotgun sequence.
ACCESSION   DAHBHC010000364 DAHBHC010000000
VERSION     DAHBHC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.369"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            160..>662
                     /locus_tag="M2A35_005605"
     CDS             160..>662
                     /locus_tag="M2A35_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201721.1"
                     /translation="MSQPNPLCVGIDVSKTTLDIATANNEFAQFTAGNDTDGFELIIA
                     ELRKHSVALVLMEATGGLEAAVACSLQAEGFDIAVINPRQARNFSRAMGYLAKTDRID
                     AKVLSQMAEVINRHPERERFIKAIPDAERQILAAMVVRRRQLITMLVAERNRLHPAHP
                     QNRKSINT"
BASE COUNT          179 a          145 c          168 g          170 t
ORIGIN      
        1 gttatcatga gcagcgaata gatttggcaa agcgtatgca gcctggtgcc ccttagggtt
       61 taagcccgta tgtaagcaaa tggcgcatac gctgagccac tcatctgaaa cccgaacagt
      121 tgtccgtaat tcataacgca tttgcaagga tgggtaataa tgagtcagcc aaatccgctt
      181 tgtgtgggta ttgatgtttc taaaacaact ctggacattg ctactgctaa caacgagttt
      241 gctcagttta ctgctggtaa cgacaccgat ggttttgagc tcatcattgc tgaattgaga
      301 aagcattcgg tggcgctggt tttgatggag gctacaggtg ggcttgaagc tgctgtagcc
      361 tgttcacttc aggctgaagg ttttgatatc gcggtcatca atcccagaca ggccaggaat
      421 ttctctcgtg ctatgggata tctggcaaag accgacagga ttgatgcaaa agtattgtct
      481 caaatggccg aggtaataaa ccgtcatcca gaacgggagc gctttatcaa agctataccc
      541 gacgccgaac ggcaaattct tgccgctatg gtggtacgta gacgccagtt gataacaatg
      601 ttggttgcgg aacggaacag acttcaccct gcacatcctc agaacagaaa gagtatcaat
      661 ac
//
LOCUS       DAHBHC010000365          658 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHBHC010000365 DAHBHC010000000
VERSION     DAHBHC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.370"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>658
                     /locus_tag="M2A35_005606"
     CDS             <1..>658
                     /locus_tag="M2A35_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCB7201722.1"
                     /translation="PDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     L"
BASE COUNT          141 a          173 c          225 g          119 t
ORIGIN      
        1 cccggacaac ggctgggaga cggcaacaga gcttgtggag gacacgcagg ccattgcccg
       61 ttacggtcgt aacgtcacga agatggatgc ctttggctgt accagtcggg ggcaggcaca
      121 ccgtgccggg ctgtggctga ttaaaacgga gctgctggaa acgcagaccg tggacttcag
      181 cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc attgaaatct gtgatgatga
      241 ctatgcgggg atcagcaccg gtgggcgtgt gctggcggtg aacagccaga cccggacgct
      301 gacgctcgac cgtgaaatca cgctgccatc ctccggtacc acgctgataa gcctggttga
      361 cggaagtggc aatccggtca gcgtggaggt tcagtccgtc accgacggcg tgaaggtaaa
      421 agtgagccgt gttcctgacg gtgttgctga atacagcgta tgggggctga agttgccgac
      481 gctgcgccag cgactgttcc gctgcgtgag tatccgtgag aacgatgacg gcacgtatgc
      541 catcaccgcc gtgcagcatg taccggaaaa agaagccatc gtggataacg gggcgcactt
      601 tgacggtgac cagagcggca cagtgaatgg tgtcacgccg ccagcggtgc agcacctg
//
LOCUS       DAHBHC010000366          656 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHBHC010000366 DAHBHC010000000
VERSION     DAHBHC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.371"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..298
                     /locus_tag="M2A35_005607"
     CDS             <1..298
                     /locus_tag="M2A35_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201723.1"
                     /translation="QFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRRAFIGLYEE
                     KQLSDNQDLHEKYFLDLMDIIDKGLGLLMKKKGIRIEPLENYFSTKSINSFDSK"
     gene            complement(276..551)
                     /locus_tag="M2A35_005608"
     CDS             complement(276..551)
                     /locus_tag="M2A35_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201724.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
BASE COUNT          204 a          127 c          143 g          182 t
ORIGIN      
        1 gcaattccag gaaatggcag cgaatatagt tcgttcccaa ctggcgactc ttgacctgag
       61 taccgtagaa aaagaaacca tcgatactat atccggtaac gtgcgtcgtg cctttatcgg
      121 tctgtacgaa gagaagcagc tctctgataa ccaggattta catgaaaaat acttcctgga
      181 tctaatggac atcattgata aggggcttgg cttgttaatg aaaaagaaag ggattcgaat
      241 agaacccctt gaaaattact tttcaacaaa aagcattaat tcttttgatt caaaataaga
      301 gaattaatta cagacttaac atgctctttc tcatgattga agctctcatg attgaaagtg
      361 ccaggttgaa gcgagtcgat ataatcaaca agactctgtc gtacgacttc atttttatcc
      421 ataacagatg caagaaatga aattgctaaa agagttatat cactacgcgc cgcagcatgc
      481 tgcaatgctt tatcaaaatt attaatctgg cgtatcaggg agttaatgat ttcatcattt
      541 tcagtcgaca tttcaccctc ctgagggttg gtgattaagg agttctccac gggtgaggtg
      601 gagtgcgtgc gccggacacg ggtgagcatc cggcactgac agtttactga aaggat
//
LOCUS       DAHBHC010000367          646 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.372, whole genome shotgun sequence.
ACCESSION   DAHBHC010000367 DAHBHC010000000
VERSION     DAHBHC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.372"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            6..227
                     /gene="kil"
                     /locus_tag="M2A35_005609"
     CDS             6..227
                     /gene="kil"
                     /locus_tag="M2A35_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCB7201725.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVIHKGRTYIASVNNIKKQRLYIR
                     DFSTQHCIKETMIKVFLGRDGLPVKAESW"
     gene            351..>646
                     /locus_tag="M2A35_005610"
     CDS             351..>646
                     /locus_tag="M2A35_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCB7201726.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAIAVATAIEAGDERRARAKFTWQF
                     LEQYPVAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEDSASFVPVETES"
BASE COUNT          187 a          139 c          140 g          180 t
ORIGIN      
        1 taattatgat tgctcatcac ttcggaactg atgagattcc tcgtcagtgc gtgactcccg
       61 gtgattatgt tattcataaa ggaagaacat atattgcttc ggtaaacaac attaaaaaac
      121 aacggctcta tattcgtgat ttttccacac aacactgtat taaggaaacc atgattaaag
      181 tcttccttgg tcgtgatggt ttacctgtaa aagcagagtc atggtgagca gtaataaaat
      241 aactgccaca atacgacatt cagcttaatg aatacatcag atttgattct tatatgccag
      301 caatggcagg gatttgttca tccttaaata tgtcatgagg ttaaaacaaa atgagtaaag
      361 tctttatttg cgccgccatt ccggacgaac aggcaataaa ggaagaaggt gcaattgctg
      421 tagccactgc cattgaagcc ggtgacgaac gccgcgcccg tgccaaattt acctggcaat
      481 tcctggagca atatccggtt gctcaggact gcgcttataa atttcttgtt tgcgaggata
      541 aacccggtat accccgccct gccctcgatt cctgggatgc tgaatatatg caggaaaacc
      601 gctgggatga ggactctgct tcctttgtcc cggttgagac tgaatc
//
LOCUS       DAHBHC010000368          644 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHBHC010000368 DAHBHC010000000
VERSION     DAHBHC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.373"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(129..>644)
                     /locus_tag="M2A35_005611"
     CDS             complement(129..>644)
                     /locus_tag="M2A35_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7201727.1"
                     /translation="QIFEDKLSGTKTDRPGLKRALKRLQKGDTLVVWKLDRLGRSMKH
                     LISLVGELRERGINFRSLTDSIDTSSPMGRFFFHVMGALAEMERELIVERTLAGLAAA
                     RARGRTGGRRPKLTKEQHEQIARLIKNGHDRKQLAIIYGIGISTIYRYHPVGDIQAEE
                     TTRQTQENENR"
BASE COUNT          137 a          175 c          143 g          189 t
ORIGIN      
        1 cgcctgcaat cctcatcgtt cagtgatatg ctgtccggct tcaaagataa ggtcacatca
       61 tcccttaaca gcgcggccag ctccgttaaa gggctgtttt gattaacgca aaaccgctaa
      121 tggtcagatt agcggttttc attttcctga gtctgcctgg ttgtttcttc agcctgtata
      181 tcgcctacag ggtgataacg ataaatcgtc gatatgccga tgccgtaaat gatcgccagt
      241 tgtttcctgt catgaccgtt tttgatcagc cttgctattt gctcatgctg ttcttttgtc
      301 agcttcgggc gacgtccgcc tgtgcgcccc cgtgcgcgcg ctgccgccag tccggccagt
      361 gtacgttcaa caattaattc acgttccatt tcagccaggg cacccatcac gtggaagaaa
      421 aaacgcccca ttggagaaga tgtatctatg ctgtcggtca gactgcgaaa attaatccct
      481 cgctcccgta gttctccgac gagagaaatc agatgtttca tgcttcgccc aaggcgatcc
      541 agtttccaga caaccagcgt gtcacctttt tgaagccgct ttaaagcgcg ttttaatccc
      601 ggtcggtctg tctttgtccc gcttaattta tcttcaaata tttg
//
LOCUS       DAHBHC010000369          642 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.374, whole genome shotgun sequence.
ACCESSION   DAHBHC010000369 DAHBHC010000000
VERSION     DAHBHC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.374"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..510)
                     /locus_tag="M2A35_005612"
     CDS             complement(<1..510)
                     /locus_tag="M2A35_005612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201728.1"
                     /translation="MAQVYEWNLSGENQHDGCGFMCSVTPTPEAQKASHMAGLVGVKG
                     GGEQTVKINGSDVKVKVTLNDITVHGALYRDTQKVVDEYLSLNDGSEVVRDSGAKVEC
                     LLLGCTYKWVTGLSGGKITLSMGIPDKVGVSSFTVPELTIATVTSKLYSDNTGAGVNN
                     EISFVYTGTI"
BASE COUNT          172 a          188 c          113 g          169 t
ORIGIN      
        1 tattgtgccg gtatacacaa atgagatttc attattaaca ccggcacctg tattatcaga
       61 atataacttt gatgtaactg ttgctatggt tagctcagga acagtaaaac tactgacccc
      121 cactttgtcc ggaatgccca tcgaaagagt tattttgcct ccggacaagc ctgtcaccca
      181 tttataagtg caccccagta ataaacactc cactttagca cctgagtccc ttaccacttc
      241 ggagccatca tttaaactta agtactcatc cactactttc tgagtatcac gataaagagc
      301 gccatggact gttatatcat tcagcgtcac tttaactttt acatccgaac cattaatctt
      361 aacagtctgt tccccgcccc ctttaacacc aaccagcccg gccatatgac tggccttttg
      421 agcttccgga gttggggtga ctgagcacat aaagccacat ccgtcatgct gattttctcc
      481 tgagagattc cattcataca cctgagccat accacccgat gcctgagcac tgctcagcca
      541 tgtagtatcc agcgtgactg acgctgaaga caggctttcc gaactgctcc ctatacttac
      601 ttctgccgcc cgcacgccgc cagccacaaa gcaggaacac aa
//
LOCUS       DAHBHC010000370          630 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.375, whole genome shotgun sequence.
ACCESSION   DAHBHC010000370 DAHBHC010000000
VERSION     DAHBHC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.375"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            24..563
                     /locus_tag="M2A35_005613"
     CDS             24..563
                     /locus_tag="M2A35_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7201729.1"
                     /translation="MPIDELKVAGVRDDRIFTDMMSGATDGREGLQRLLARAEKDDII
                     LCTKMDRLGRNTADMITIVDTCYKKGIAIRFLENGLSTEGTMGKMVIQILAAVTEAER
                     ERILERTNEGRIAAMASGVKFGRKPHRQSDMVRELITQDAPEKTILEKTGVSRATFYR
                     LKKRTRIEQIGVIREKTKR"
BASE COUNT          195 a          115 c          172 g          148 t
ORIGIN      
        1 tttttaatct tttgggggtt taaattccta ttgatgaact caaagtggcg ggcgtgagag
       61 atgatcggat tttcactgac atgatgtcgg gagcaaccga tgggcgagaa gggttgcagc
      121 ggcttcttgc tcgagcagaa aaagatgaca ttattctttg taccaaaatg gaccgccttg
      181 gccgcaatac cgctgatatg atcactatcg tagatacctg ttataaaaag ggtatcgcta
      241 ttcgatttct tgaaaatggc ttaagcaccg aggggacgat gggcaagatg gtcattcaaa
      301 tactggctgc tgtgactgag gcggaacggg agcgcattct ggagcggact aatgaaggtc
      361 gaatagcggc catggcatct ggagtgaaat tcggacgtaa accgcaccgt cagtctgaca
      421 tggtgcggga gttaatcaca caagatgcac cagagaaaac tatactggaa aaaacgggcg
      481 tatcgcgggc gaccttttac cgactgaaga aaaggactcg gatagagcaa atcggggtta
      541 tacgtgaaaa gacgaaaaga taagacgatg ttaaaaatgg aagaaaaaaa attaatatca
      601 accgaattca cttcctttat gaagcgttgg
//
LOCUS       DAHBHC010000371          620 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.376, whole genome shotgun sequence.
ACCESSION   DAHBHC010000371 DAHBHC010000000
VERSION     DAHBHC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.376"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(42..452)
                     /locus_tag="M2A35_005614"
     CDS             complement(42..452)
                     /locus_tag="M2A35_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201730.1"
                     /translation="MHAKEEGIIRALKEISKMESEVAKKAVANAHMDVATHTMIVAKV
                     TAEAAKIIEEQGVELTLLKTKPVTGLDLSDTGRLIYTIGSEPQRYTIIAGLQNKYLIT
                     PHPIRESALLTNLRLIERSQVAFIDDARHTVFNA"
BASE COUNT          131 a          130 c          141 g          218 t
ORIGIN      
        1 aaaatgctta attgccattg cgcccccttt gtccagtaac cctatgcgtt aaatacggtg
       61 tgtctggcgt catcaatgaa tgcaacttga gagcgctcta tcaggcggag atttgttaga
      121 agcgctgatt cccttatggg gtgaggagtg atcaggtatt tgttctgtaa tccggcgata
      181 atggtgtatc gctgtggctc cgagccaata gtgtaaataa ggcgtccggt gtcagataaa
      241 tccagtccgg tgactggttt agttttgaga agcgtaagtt ccacaccctg ttcttcgatg
      301 attttggcag cttctgccgt gacttttgcg actatcattg tgtgggttgc gacgtccatg
      361 tgagcattgg ccacggcttt tttcgctact tcgctttcca tctttgaaat ttctttcagt
      421 gctctgatga taccttcttc ttttgcgtgc atttttgtat ctccgttatt tgcgtgtgcg
      481 aatacctccg ttaatacgga tggttttcac gttttcttat ttaatttgat gttttatttg
      541 tatcgttatt catcagagaa aaaacgctcg atctttttca ctgaattaat aattcgcata
      601 atcccaatgg cgcaggccac
//
LOCUS       DAHBHC010000372          620 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHBHC010000372 DAHBHC010000000
VERSION     DAHBHC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.377"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
BASE COUNT          203 a          144 c          102 g          171 t
ORIGIN      
        1 ccgggagtca catttttctg catatctgat actttggttc ccactactta tttggtggac
       61 accactttgt ctaattcgtc agattctgac cagacggttc aggctgtacg cttacccacc
      121 aactaataat atctcagcag atatttcctg accagggtga cacatttatt aaagattttt
      181 aacaggacac ttcacattac tgaacgctat tccgacagta tcactacaca aataaaaaag
      241 ggaactggat aaatattcat gccggaagaa tggtgtaaca aaagcattta atcattacgt
      301 gcgtattcct ctctattcga tcacggtttg gccaaaccgt gatcgattca gatgccattt
      361 tgcaagtacg catgacccgg aagggaaaca cttaacaaca tggtatcaac agatatcatt
      421 acgtgcgcac tcttataaaa atacacagca gaagcaagat aactctaaac gtaaatttaa
      481 tatctcccac tgactgcccc gacaatatac tgaaaaatac tcacctcagt aaacataaac
      541 acacctgata atcaggctgc aagatgaata tttttctcct ttacggagta atcagcctgc
      601 tgtttaacaa taaccgcgta
//
LOCUS       DAHBHC010000373          614 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHBHC010000373 DAHBHC010000000
VERSION     DAHBHC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.378"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..518
                     /locus_tag="M2A35_005615"
     CDS             <1..518
                     /locus_tag="M2A35_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201731.1"
                     /translation="PGPVLTRDIERVLERSGEAPVYGPNLRASCRRMEASDWLRTLRA
                     PNLQLAVELTDAGRAVAQPLLQAEQERLRAEQRAAEVVVLPLVPAADLPADGTSAKDL
                     PVDLDGMTYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGME
                     VRVQVNESTNP"
BASE COUNT          123 a          180 c          202 g          109 t
ORIGIN      
        1 ggccgggccc agtgctgaca cgggacatag aacgggtgct ggagcgaagc ggggaggccc
       61 ctgtctacgg gccaaacctg cgcgcctcct gccggcgcat ggaagcatca gactggctgc
      121 gcaccctgcg ggccccgaat ctgcagctgg ctgtcgagct gacggacgcc ggccgggccg
      181 tggcacagcc tctgctgcag gctgaacagg agcgcctccg ggcagaacag cgcgcggcgg
      241 aagtcgtcgt actgccgctg gtcccggcag cagatctgcc cgcagacggc acgtctgcca
      301 aagacctgcc ggtggatctg gacggcatga cgtatcaggc ctgccgtggg gattttgtgg
      361 tgcgtctcga cggcagcacc tgtctgcagt tatggaataa agaaggcaga gtggtacgcc
      421 gggagggcga tccactggaa gtggcgcagt ggctgcaggc ctgtcacgat gcaggaatgg
      481 aggttcgtgt gcaggttaat gaaagcacca acccgtaaaa acatgcctgc acggtttaca
      541 cgtcatccgt aaccggctca tttaaaccgt ctggtctgtt tcctccggct ccacaaaaat
      601 aatgtccatc attt
//
LOCUS       DAHBHC010000374          614 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.379, whole genome shotgun sequence.
ACCESSION   DAHBHC010000374 DAHBHC010000000
VERSION     DAHBHC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.379"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>614
                     /locus_tag="M2A35_005616"
     CDS             <1..>614
                     /locus_tag="M2A35_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201732.1"
                     /translation="WVSNEQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVV
                     SEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGTYPNQVNTKDNIKPYSSENSGE
                     SSDLPENDLPVVKADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWAND
                     IRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQL"
BASE COUNT          180 a          137 c          164 g          133 t
ORIGIN      
        1 tgggtgagca acgagcaact ttccgagttg accgggatat tgccgcacaa gtgttctgct
       61 gcaaaaagtg ttctggtaaa gcgtgggatt tttattcaga gcgggcggaa tatcggcatt
      121 aataatgtgg tcagtgaatg gtcaacatta cccgaatcag gtaagaaaaa taaagtttac
      181 ctaaaagagg taaatttacc tgaatcaggt aagaaaagtt tacccaaatc aggtaaaggc
      241 acttacccga atcaggtaaa cacaaaagac aatataaaac cttattcgtc cgagaattct
      301 ggcgaatcct ctgacctgcc agaaaacgac cttcctgtgg tgaaagcgga tgctgcgatt
      361 cagagcggca gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac
      421 atggtgaaga ccatcgcgcc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
      481 atccgtctga tgcgtgaacg tgatggacgt aaccaccgcg acatgtgtgt actgttccgc
      541 tgggcctgcc aggacaactt ctggtccggt aacgtgctga gtccggccaa actccgcgac
      601 aagtggaccc agct
//
LOCUS       DAHBHC010000375          612 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.380, whole genome shotgun sequence.
ACCESSION   DAHBHC010000375 DAHBHC010000000
VERSION     DAHBHC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.380"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            78..>612
                     /locus_tag="M2A35_005617"
     CDS             78..>612
                     /locus_tag="M2A35_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCB7201733.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFYGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVHHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKAC"
BASE COUNT          138 a          167 c          195 g          112 t
ORIGIN      
        1 tggtcactgg cctgaagttc gccgacagct aatcaactca cggcgggcat catgcccgcc
       61 tgtaacggag agaaaaaatg attactcctg cacagcaaca ctggcagaac gtgatggcac
      121 agcgcgcagg ccgggcgaat gaaggcgtgg accacgccgc gcgtaccgcg catgaagagg
      181 tgctgtatcg tctgcgtctg gcacaggccc ggcttaaggg cgtacaggcc agaagcgcga
      241 aagccgccat caaaaaagag ttgttgccgg acttttacgg ctggattgag ggaacgctgg
      301 aggctgacgg cgggcagcag gatgaagtga ttgccacgct gatggtgtgg gcgattgact
      361 gcggcgatct tccgctggcg ctgcgtattg gtgcgtatgt ggtccatcac aacctcatca
      421 tgccggataa ctttggccgt actgctgcca cagtgctgac cgaagaaatc tgcaatccgg
      481 tactgacgca ggccgggacg gatgccgacg cggatttgtc cgcctttatc gaaccactgg
      541 acaccctccg ggagattgtc accgaccagg acatgccgga cgaagtgcgc gccaaattat
      601 gcaaggcgtg cg
//
LOCUS       DAHBHC010000376          607 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.381, whole genome shotgun sequence.
ACCESSION   DAHBHC010000376 DAHBHC010000000
VERSION     DAHBHC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.381"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..401
                     /locus_tag="M2A35_005618"
     CDS             <1..401
                     /locus_tag="M2A35_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7201734.1"
                     /translation="TAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNNVVIQNDGTNGLPGPQMM
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            398..>607
                     /locus_tag="M2A35_005619"
     CDS             398..>607
                     /locus_tag="M2A35_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201735.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKA"
BASE COUNT          128 a          142 c          212 g          125 t
ORIGIN      
        1 gtacagccat tcaggcagct gcggcgaaat tccattttgc gaccggggga tttacgggaa
       61 ccggcggcaa atatgagcca gcggggattg ttcaccgcgg ggagtttgtc ttcacgaagg
      121 aggcaaccag ccggattggc gtcggcaacc tgtatcgtct gatgcgcggg tatgcggaag
      181 gtggttatgt gggcggtgcc ggaagtccgg cgcagatgcg gcgggctgaa ggcattaatt
      241 ttaatcagaa caacaacgtg gtgattcaga acgacggtac gaatggtctg ccaggtccac
      301 agatgatgaa ggcagtgtat gacatggccc gcaagggtgc ccgtgatgaa attcagacac
      361 agatgcgtga tggtggcctg ttctccggag gtggacgatg aaaaccttcc gctggaaagt
      421 gaaacccggg atggatgtga catcggctcc ttccgtcagg gaggtgcgct ttggtgatgg
      481 ctattcccag cgtgcgcctg ccgggttgaa cgctgacctg aaaacgtaca gcgtgacgct
      541 gtctgtctcc cgtgaggagg ccacggcgct ggagtcgttt ctggctgagc acgggggctg
      601 gaaggcc
//
LOCUS       DAHBHC010000377          607 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.382, whole genome shotgun sequence.
ACCESSION   DAHBHC010000377 DAHBHC010000000
VERSION     DAHBHC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.382"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>607
                     /locus_tag="M2A35_005620"
     CDS             <1..>607
                     /locus_tag="M2A35_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7201736.1"
                     /translation="FPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCND
                     HISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQ
                     FIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTIFD
                     RLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKA"
BASE COUNT          219 a          116 c           94 g          178 t
ORIGIN      
        1 tattcccttt cactacttcg ttgaatacac tggatccatt aatacttaat caggaattag
       61 tgcaaatcat ttcaaataaa aaaatcgatg gtctctatac ctatccgatg gctgcgacaa
      121 atttttgtaa tgatcacata tctcaaaatt cattcttgag cagattcaag ccagattatt
      181 tcgttacagg aagaataaac cagaataatg cagtgaacac tttatacatt gagttgatcg
      241 acgctaaaaa ccttttcctc atcgccagta atcatctccc tgttgatgaa ctacataata
      301 catcacaatt tattatagat aatatccttc aaacggtaca taaaccagaa cgatctgtaa
      361 gattagctaa gcaggaccaa ggatataaga atcattattt atcagatgaa atgttagccg
      421 gaaagaaaga actttacgac ttcacccctg aaagcattta cagggccatg actatatttg
      481 atagattaca aaataaaagt gatatacaga cgctaaaaac agaatgttat tgccttctag
      541 cggaatgcca tatgtctttg gcacttcatg gaaaaagtga acttgaactt gctgctcaaa
      601 aagcatt
//
LOCUS       DAHBHC010000378          604 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHBHC010000378 DAHBHC010000000
VERSION     DAHBHC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.383"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..199)
                     /locus_tag="M2A35_005621"
     CDS             complement(<1..199)
                     /locus_tag="M2A35_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201737.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGV"
     gene            complement(203..>604)
                     /locus_tag="M2A35_005622"
     CDS             complement(203..>604)
                     /locus_tag="M2A35_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201738.1"
                     /translation="GNATGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGID
                     LSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRA
                     TTGDAQRPNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          130 a          172 c          146 g          156 t
ORIGIN      
        1 agaccccctt caccttgtct gcggtatcac catctgccag cggcacgaaa aaaccgtcat
       61 catcgtattt accagccagc ccataggcag caaaggcgtt atcggattta aggactaccg
      121 gttcgacggt taagtcctgc gggcgagaga cagccccggc aatgccaaca ggcatccggt
      181 acagaaatac attattcatt ttttaccctt tacggtttgc ccagaattca gcgttttgtt
      241 tgttcaggga agcgatactg gtcatgccca tatttgggcg ctgtgcatcg ccagtggtgg
      301 cgcgggtgtt tcgccctttg gcaatctcag acacggcatt aaacgccatg tcgaccgatt
      361 gtttcggcaa tttgcggata tccgcatcac cgactatctg gcgaaccagc gttttgtcag
      421 cagaagccag aacctcacgt ttgaacgcgg tcggtttcat cttacggctc agatcaatac
      481 ccggaacgat aacttcggca cgccaggctg agtcaccagt aatcgtggtt tcctcttcat
      541 cgtcctcgcc gtcaccggtc ggattatcgt caggcttatt gtcgttatcg cccgtcgcat
      601 ttcc
//
LOCUS       DAHBHC010000379          587 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.384, whole genome shotgun sequence.
ACCESSION   DAHBHC010000379 DAHBHC010000000
VERSION     DAHBHC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.384"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>587)
                     /locus_tag="M2A35_005623"
     CDS             complement(<1..>587)
                     /locus_tag="M2A35_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCB7201739.1"
                     /translation="QSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAA
                     GKKGRAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQ
                     IGATWYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKG
                     GDKIILSNGAELHFLGTSAASAQSYTGNFYFDEFF"
BASE COUNT          133 a          163 c          140 g          151 t
ORIGIN      
        1 cagaaaaatt catcaaaata aaaattgccc gtataggact gtgccgacgc agcagaagtg
       61 ccgagaaaat gcagctctgc gccgttggag aggatgattt tatcgccccc tttcagctcc
      121 acatcaactt cagccgcggc cttctgaata atgcttttaa actggaacgc ctgacgacgc
      181 gacgcagaca aaaaaatctg gttacgctgg taaggttgcg ccacatcgtc acgcagcgcc
      241 atcagcagag cttcctgtgc aaaataccag gtcgccccaa tctgtcggga tttcaggatc
      301 atcctgttac gtatcccggc ttccctgcaa agggtcaggg agtcaaacca gccccgctga
      361 tgccactcca gcctgctgat gattttttcc cgcagtgcgg caatctgttc cggcgtgaaa
      421 tgatttttga gttttttcgc ccggcctttc tttcctgcgg ccatcacatc cggctggcca
      481 tcatgcagct ttttaagctg ccgggtcagc aggtctattt ccttaaagtc accgcctgtt
      541 ttattctgtt tttcagtaag ctggatgagg cgcgcatcga tggactg
//
LOCUS       DAHBHC010000380          585 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHBHC010000380 DAHBHC010000000
VERSION     DAHBHC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.385"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>585)
                     /locus_tag="M2A35_005624"
     CDS             complement(<1..>585)
                     /locus_tag="M2A35_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201740.1"
                     /translation="DRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTV
                     SKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCR
                     LRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTL"
BASE COUNT          131 a          149 c          141 g          164 t
ORIGIN      
        1 cagtgtcccc tgtccggcag gcatatctat aactgaggaa aatacgccct gagcatccgt
       61 cgtggactga ttaaaaatct cctgaccatt gcgggtcact cttaaccggc aggttgaata
      121 ccagtatgac tgttggttat tactgttgaa attctcatgc ttaccaccgc ggaataacac
      181 tggcggtatc atgacctgcc ggtcaaattt ctgatcatca ctgattctta ccgtgatggt
      241 gccactggca taagtgctcg tgcgggggaa agacttgctg accgttttga caatatcgcc
      301 ttcaatctga ttggctgaca gtttcccctt aatctgacag ttctcattaa tcgtgacgtt
      361 gttgagcgtc ccggagttcg cattcacact gccactgata tccgcatttt tagcggtcag
      421 ctttccgtcc ggtgtcaggg aaaaggccgg aggattgccg ccgctggtaa tggtgggggc
      481 cgtcaggcgc ttcaggaaca cttcgttcat gaatatctga tcaccctgac caacaaacat
      541 cggctttgtg ttgccatttg caggattaat catcgcaatc ctgtc
//
LOCUS       DAHBHC010000381          572 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.386, whole genome shotgun sequence.
ACCESSION   DAHBHC010000381 DAHBHC010000000
VERSION     DAHBHC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.386"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>572
                     /locus_tag="M2A35_005625"
     CDS             <1..>572
                     /locus_tag="M2A35_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HCB7201741.1"
                     /translation="ALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSN"
BASE COUNT          177 a           93 c          120 g          182 t
ORIGIN      
        1 cgcactcatg atcgtaatgg cgattattcc tggctttcct acattggtct tcttgtttct
       61 ggcggtttgc ctgttgggta tcgcctggaa attacaaaag aaaagaacgt ttggaaccgg
      121 aaataataag gacgctatgg gagctgattt gtctaatagt caaaatatct caccgggtgc
      181 cgagccgttg attttaaact taagtagtaa tatttatagc tcagatatta cacagcagat
      241 tgaggttatg cgttggaatt tctttgagga aagtggaatt ccattgccta agattattgt
      301 taatccagtt aaaaataatg atagcgctat agaatttttg ctctatcaag agtcaatata
      361 caaagatact cttacagatg atactgttta ttttgaggct gggcatgcag agatatcatt
      421 tgaatttgtc caggaaaagc tttcggctaa ctctatcgtg tataaaacga atgaggctaa
      481 ccaacaacta gctcacgtct caggtatgga tgtttatgca aaaacaaatg acaagataac
      541 gtttttgcta aagaaacttg tattgtctaa tg
//
LOCUS       DAHBHC010000382          570 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHBHC010000382 DAHBHC010000000
VERSION     DAHBHC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.387"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..478
                     /locus_tag="M2A35_005626"
     CDS             <1..478
                     /locus_tag="M2A35_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCB7201742.1"
                     /translation="WTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWD
                     EDKNSIRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGY
                     SGVAARIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            490..>570
                     /locus_tag="M2A35_005627"
     CDS             490..>570
                     /locus_tag="M2A35_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCB7201743.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRS"
BASE COUNT          136 a          160 c          167 g          107 t
ORIGIN      
        1 atggacacca cgcagacgcc gcgtaacccg gatacaggaa cgcattcgct ttatctggaa
       61 taacgaagca cgcacgctga aaaactggca tcacgacacg gggaaatacg ggcgaaccat
      121 caccgggtgg gatgaggata aaaacagcat ccgcacgttt taccgggatg acatcgaccg
      181 ctttctggaa atacgcaccc ggcgcatcaa ccaggacagc acaaagcgcg tccccatgtt
      241 cgtaaaactg cgcaccgccc gctacctgaa agcccgtgca gatgcttccg gtgtgacggt
      301 gggttacagc ggcgtggccg cacgtattgc ccgcgttcat cagttcggtg agcgcgatca
      361 ggttgcgccg ggcattttca ccgattaccc ggtacgtgag ctgttgggca tcagtcaggc
      421 agatgaacgc ctgatttata acacagtgct gggccggatt gcggaggctg tacggtgagc
      481 gcagaactca tgcgactgct gagcaatatc atccgcaccg ggatcatctc tgaagttgat
      541 gagaagtcct ggcgcgtgcg cgttcgcagc
//
LOCUS       DAHBHC010000383          564 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.388, whole genome shotgun sequence.
ACCESSION   DAHBHC010000383 DAHBHC010000000
VERSION     DAHBHC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.388"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..500
                     /gene="istB"
                     /locus_tag="M2A35_005628"
     CDS             <1..500
                     /gene="istB"
                     /locus_tag="M2A35_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCB7201744.1"
                     /translation="ALIRSLSQGNWLTLKQNILLTGATGSGKTFLACALGHNACRQGY
                     KVYYYRLKALMEQCYQGHADGRYSKLLTRLNNSDLLLLDDWGLEPLSSEQRSDLLEIV
                     DLMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRLVHGSIKIELKGESMRKIQSPL
                     TEGDQ"
BASE COUNT          164 a          129 c          144 g          127 t
ORIGIN      
        1 gggccctcat ccgttcactc agtcagggaa actggttaac cctgaaacaa aatattttac
       61 tgaccggggc caccggcagc ggtaaaacgt tcctggcatg tgcacttggt cataatgcct
      121 gccgacaggg atacaaggtc tactattatc gccttaaagc gctgatggaa cagtgctatc
      181 aggggcatgc tgatggaaga tacagcaaac ttttgaccag gctgaataat agcgatctgc
      241 tgcttctgga tgactggggg ctggaacctc tctcatcaga acagcgtagc gacctgctgg
      301 aaatagtgga tctgatgtac caacgaggct caatcatcgt agtgagccag ttgccggtgg
      361 aaaactggta caaaatgatc ggagactcca cacatgcgga tgccatccta gatcgactgg
      421 ttcatggcag tatcaagatc gaacttaaag gagaatcaat gcggaaaata caatctccgt
      481 tgaccgaagg agatcagtga aggtaattta aaaacggttc tgtgaaagtg acacgaaccg
      541 atctccatcg atgttactca ccga
//
LOCUS       DAHBHC010000384          560 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.389, whole genome shotgun sequence.
ACCESSION   DAHBHC010000384 DAHBHC010000000
VERSION     DAHBHC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.389"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>560
                     /locus_tag="M2A35_005629"
     CDS             <1..>560
                     /locus_tag="M2A35_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201745.1"
                     /translation="QEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRR
                     IAQMEADLNRLQKESDTLTGRVYDPAVQRLLRQTRTRKPFPESLPRDEKRLLPAAPCC
                     PNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIA
                     GPGLLARVLTSKYAEHTPLYRQSEIYG"
BASE COUNT          125 a          169 c          169 g           97 t
ORIGIN      
        1 caggaagcgc tgatccacgc cctgcaggaa aagctgagca accgggagcg cgaaatagac
       61 catctgcagg cgcagctgga taaactccgc cggatgaact tcggcagtcg ttccgaaaaa
      121 gtctcccgcc gtatcgcaca aatggaagcc gatctgaacc ggcttcagaa agagagcgat
      181 acgctgactg gtagggtgta tgacccggca gtacagcgtc tgttgcgtca gacccgcacc
      241 cgtaagccgt tccctgaatc actaccccgt gacgaaaagc gactgttgcc tgcggcgccg
      301 tgctgcccga actgcggcgg ttcactgagc tatctgggcg aggataccgc cgaacagctg
      361 gagttgatgc gtagcgcctt ccgggttatc cggacggtac gggaaaaaca tgcctgtact
      421 cagtgcgatg ccatcgtgca ggcacctgca ccttcgcggc ccatcgagcg gggtatcgcc
      481 ggaccggggc tgctggcccg cgtgctgacc tcgaagtatg cagagcacac cccgctgtat
      541 cgccagtcag aaatatacgg
//
LOCUS       DAHBHC010000385          559 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHBHC010000385 DAHBHC010000000
VERSION     DAHBHC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.390"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            52..417
                     /locus_tag="M2A35_005630"
     CDS             52..417
                     /locus_tag="M2A35_005630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201746.1"
                     /translation="MARSAKPRKRKPAPQRSKLSRYVVTLHDDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDVGNKKLVFWFVRVDGEGYPEIARCTEREFATMLAGISAGG
                     MYCPECGTVHWPDGVTPPF"
     gene            417..>559
                     /locus_tag="M2A35_005631"
     CDS             417..>559
                     /locus_tag="M2A35_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCB7201747.1"
                     /translation="MHPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMG"
BASE COUNT          130 a          134 c          159 g          136 t
ORIGIN      
        1 tgagattacc gttataaaaa caaacaatat cattgtcttt caggtagtta tatggcccgt
       61 tcagctaaac cccgaaaacg aaaacccgcc cctcaaagaa gcaaactctc ccgctatgtc
      121 gtgacgcttc acgacgatga cttctttgac gaagaagacg cagaagttct gcgctttgat
      181 agttttgacg atgccgttga atgctgcgca gacctgaata ttccgttctt tgtggatgtg
      241 ggaaacaaaa agctggtctt ctggtttgta cgtgttgatg gcgaagggta tccggaaata
      301 gcccgctgca cggagcggga gtttgcgacc atgcttgccg gtatcagcgc cggcggtatg
      361 tactgtccgg aatgcggcac ggttcactgg ccggacggag tcaccccgcc cttctgatgc
      421 atccccgttt tgccgatatt tttcagcagg gaaaccgctg gcttaactgg ctggagaagc
      481 agccggaagg gtctgtgcgt ccggtggtga ttgagtcggt gacaaaaatc atggcgtgcg
      541 ggaccacgct gatggggta
//
LOCUS       DAHBHC010000386          556 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.391, whole genome shotgun sequence.
ACCESSION   DAHBHC010000386 DAHBHC010000000
VERSION     DAHBHC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.391"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>556
                     /locus_tag="M2A35_005632"
     CDS             <1..>556
                     /locus_tag="M2A35_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCB7201748.1"
                     /translation="KVCFRCKSRSCPHCGVKAGAQWIQYLLSLAPDCPWQHIVFTLPC
                     QYWSLIFHNRWLLAEMSRIAANVILEICRQADVEPGIFTVIHTWGRGQQWHPHIHLST
                     TAGGVTSAHTWKNLHFYARKVMRMWRYRITRLLSRKYPELVIPAELAAEGSGRREWNR
                     FLDTHYRRGWNVNVSRVMDNATHVA"
BASE COUNT          121 a          151 c          172 g          112 t
ORIGIN      
        1 aaggtctgct tccggtgtaa aagtcgctcc tgcccgcact gcggggtgaa ggctggcgca
       61 cagtggatac agtatctgct gagtctggct cccgactgcc cgtggcagca tattgtgttc
      121 acacttccct gccagtactg gtccctgata ttccacaacc ggtggttgct ggcagagatg
      181 agccgtatcg cagcgaatgt gatactggaa atctgccgtc aggcggacgt ggagccgggg
      241 atattcacgg taatccacac atggggacga ggccagcagt ggcatccgca catacacctg
      301 tcgacaacag ccggcggtgt gacgtcagct cacacctgga aaaaccttca tttttacgcc
      361 cgtaaggtga tgcgcatgtg gcgttaccgg ataacgcgat tactgtcccg gaaatacccg
      421 gagctggtga taccggctga gctggcagca gagggaagcg gcagacggga atggaatcgc
      481 ttcctggaca cccactaccg gcgtggctgg aatgtcaacg tatcccgggt gatggataac
      541 gccacgcatg tggcgg
//
LOCUS       DAHBHC010000387          556 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.392, whole genome shotgun sequence.
ACCESSION   DAHBHC010000387 DAHBHC010000000
VERSION     DAHBHC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.392"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..411
                     /locus_tag="M2A35_005633"
     CDS             <1..411
                     /locus_tag="M2A35_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCB7201749.1"
                     /translation="ASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKE
                     ATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGKFEQNNHVVINNDGTNGQIG
                     PAALKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
     gene            408..>556
                     /locus_tag="M2A35_005634"
     CDS             408..>556
                     /locus_tag="M2A35_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201750.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRAPAGLNADLKTY
                     SVTLSV"
BASE COUNT          115 a          136 c          190 g          115 t
ORIGIN      
        1 gcgtcaggcg gtacagccat tcaggccgct gcggcgaaat tccattttgc aaccggagga
       61 tttacgggaa ccggcggcaa atatgagcca gcggggattg ttcaccgcgg ggagtttgtc
      121 ttcacgaagg aggcaaccag ccggattggc gtggggaatc tttaccggct tatgcgcggc
      181 tatgccaccg gcggttatgt cggtacaccg ggcagtctgg cggacagccg gtcgcaggcg
      241 tccgggaagt ttgagcagaa taaccatgtg gtgattaaca acgacggcac gaacgggcag
      301 ataggtccgg ctgctctgaa ggcggtgtat gacatggccc gcaagggtgc ccgtgatgaa
      361 attcaggcac agatgcgtga tggtggcttg ttctccggag gtggacgatg aaaaccttcc
      421 gctggaaagt gaaacccggg atggatgtga catcggctcc ttccgtcagg gaggtgcgct
      481 ttggtgatgg ctattcccag cgtgcgcctg ccgggctgaa tgctgacctg aaaacgtaca
      541 gcgtgacgct ttctgt
//
LOCUS       DAHBHC010000388          555 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.393, whole genome shotgun sequence.
ACCESSION   DAHBHC010000388 DAHBHC010000000
VERSION     DAHBHC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.393"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..295)
                     /locus_tag="M2A35_005635"
     CDS             complement(<1..295)
                     /locus_tag="M2A35_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201751.1"
                     /translation="MAKNDFKAFATDRNANVISQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFG"
     gene            complement(295..552)
                     /locus_tag="M2A35_005636"
     CDS             complement(295..552)
                     /locus_tag="M2A35_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HCB7201752.1"
                     /translation="MQIVDNQDMTISVWVFPDTDISDVSRELIAAIKQGYLTVKAAGV
                     WAGGIETPSVETPSEGSKFFGFDMDNEFISGFDVGAWGVLL"
BASE COUNT          153 a          159 c          102 g          141 t
ORIGIN      
        1 cgccaaacgg attcttgcgg ctcaggtatt caacagcaaa ccccgatccc agcaattcaa
       61 caaaaccggg cagatcacca ttatcaagca catcccgttg cgttttatca cttacaaact
      121 gggccagagc tgcagcaata aagctggcct gccgaataac cttattgact tgcgcactgg
      181 aggctttccc tgctgtaaat ccagataaaa gcgcgggcaa cgcttcccat tcctcctgcg
      241 atataacatt ggcatttcga tccgttgcaa acgctttaaa gtcatttttc gccatcagag
      301 taatactccc catgctccta catcaaaacc actgatgaat tcgttatcca tatcaaaacc
      361 aaaaaatttt gagccttccg atggggtttc caccgaaggt gtttcaatgc cacccgccca
      421 taccccggcg gcttttactg tgagataccc ctgtttaatt gccgcaatta actcacgcga
      481 tacatctgaa atatcagtat caggaaagac ccagaccgat atcgtcatgt cctggttatc
      541 gactatctgc attcg
//
LOCUS       DAHBHC010000389          554 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHBHC010000389 DAHBHC010000000
VERSION     DAHBHC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.394"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..357)
                     /locus_tag="M2A35_005637"
     CDS             complement(<1..357)
                     /locus_tag="M2A35_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCB7201753.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAAL"
     gene            complement(384..>554)
                     /locus_tag="M2A35_005638"
     CDS             complement(384..>554)
                     /locus_tag="M2A35_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCB7201754.1"
                     /translation="VLTTWPADAIARAEDVVLRLSGMSGPVHVDTDITEVAKNNALTD
                     DDFSAGKSSTAS"
BASE COUNT          138 a          179 c          129 g          108 t
ORIGIN      
        1 taatgcggca tcatccacca tgacatccgc cacatccgca gacgataaaa tatcgcgccc
       61 tccgtcccca ccgaaccgga cgcctccggc aagtcctgcc gctttctgca tcagcatttt
      121 gtcctcatcc ggcctctcca cctgctcttc ctcatgccgg gggacaagca gactgaaatc
      181 agagggatgc atatccggat cgcaaaaaaa caggctgagt acagcgtacg tcagcccgga
      241 aaaatgcata tccagctggg tatcgtgaaa ataatgcatg cggtaaaaat gtcgccagtc
      301 ggcatattcg gtggatgtca tcccggcaag catggcgcgc cagtcaggcc tccccatctc
      361 acgcgccagt ctgagggcaa aattcagctc gccgtcgaag actttcccgc agaaaaatca
      421 tcatcagtca gcgcgttatt tttcgccact tcggtaatat ccgtatccac atgaacaggc
      481 ccgctcatcc cggacagacg caacaccaca tcttccgccc gggcaatggc atcagcaggc
      541 caggtggtca ggac
//
LOCUS       DAHBHC010000390          542 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.395, whole genome shotgun sequence.
ACCESSION   DAHBHC010000390 DAHBHC010000000
VERSION     DAHBHC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.395"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(351..512)
                     /locus_tag="M2A35_005639"
     CDS             complement(351..512)
                     /locus_tag="M2A35_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCB7201755.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGCREVTA
                     TMAYESGGK"
BASE COUNT          144 a          148 c          151 g           99 t
ORIGIN      
        1 acgcccggaa aaggcacagg caggagaaca acagaactga cagtaatcag ggcagcagta
       61 atcagagtca gaacaggagg aaaaagagaa taatcacagg gaagctgatg tgatttctga
      121 ctggccataa ccagagaagg cgggggagaa gaacgggcgc cgcaggcgca cgttcattcc
      181 cgttcccgga gggaaacagc cttcccggca gtctggttgt tcatgcccgt acaatgccag
      241 caaggagaaa ggggccaccg gcgaaccagc agccccttta taaaggcgct tcagtgcagt
      301 cagaccagca tcagccctga aaaggcgggc ctgcgcccgc ctccaggttg ctacttaccg
      361 ccggattcgt aagccattgt tgccgtaact tccctgcatc cgtccttcag ccggacttcg
      421 cacaggcgat tccgggtcag gagtgtgaat atcaacaacg tgcagcacac gattaatacg
      481 caccagataa gggcgtttcc cggtagtttc atggtttact cctccttgca atttgcacgg
      541 ta
//
LOCUS       DAHBHC010000391          541 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHBHC010000391 DAHBHC010000000
VERSION     DAHBHC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.396"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            33..>541
                     /locus_tag="M2A35_005640"
     CDS             33..>541
                     /locus_tag="M2A35_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCB7201756.1"
                     /translation="MYRDILPELDLVLWLIKADDRALSVDEYFWRQILQCGHQQVLFV
                     VTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDT
                     LVSALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVA
                     RTVLRAVRDS"
BASE COUNT          107 a          153 c          168 g          113 t
ORIGIN      
        1 agagccggga cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaactgg
       61 acctggtact gtggctgatt aaagccgatg accgtgccct gtctgtggat gagtatttct
      121 ggcgacagat cctgcagtgc ggacatcagc aggtgctgtt tgtggtgacg caggccgaca
      181 aaacggaacc ctgccatgaa tgggatatgg caggtattca gccttctccc gcacaggaac
      241 agaacattcg tgagaaaaca gatgcggtat tccggctgtt ccggcccgta catccggttg
      301 tggccgtatc ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag
      361 cacttcccga ccatgccgcc agccccctga tgacccgact gcaggacgag ctgcgcacgg
      421 agtctgtccg ctcgcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca
      481 cggcggagag cgtctgtatt gcctctgttg cacgcacggt tctgcgggcc gtccgtgact
      541 c
//
LOCUS       DAHBHC010000392          539 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHBHC010000392 DAHBHC010000000
VERSION     DAHBHC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.397"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>539)
                     /locus_tag="M2A35_005641"
     CDS             complement(<1..>539)
                     /locus_tag="M2A35_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201757.1"
                     /translation="EDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHR
                     LVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGD
                     RLTTVQTDTTRIQTVYQPGSFTPLIRVETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVGLLDRLEGEIRVN"
BASE COUNT           73 a          194 c          145 g          127 t
ORIGIN      
        1 gtttacccgg atttccccct ccagcctgtc cagcagcccc accagttcag ccgggaacac
       61 cacaccgtga ccgtcttcgc tcccttcctg ctgtaacttc tccgccaggc tgcggcgctg
      121 cgctttctcc cgctcgccgt tctctgtctc gacccggatg agcggcgtga agctccccgg
      181 ctggtataca gtctggatac gcgtggtgtc ggtctgtacc gtcgtcagcc tgtcgccgtc
      241 ccagccgtac catgtctcct ccggtttacg cgacagcgac atccaccccg tcaggtcacg
      301 ctcccgccgc catacccgtt tcgccattcg ccgtcccagc gggtcgtaga ggtagcggct
      361 ctcgaccagt ggctcgccat gctgtatccg cgtgtggaac accaggcggt gctggctgtc
      421 gtagtggtag tggtgggtcc gctcgtcgtc cgtccgtatc acacccgtcg ggatacggtc
      481 cgtcttctcc gtcagtctgc cgtattcatc atagtggtaa acatagtgcg catcctctg
//
LOCUS       DAHBHC010000393          537 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHBHC010000393 DAHBHC010000000
VERSION     DAHBHC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.398"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..285)
                     /locus_tag="M2A35_005642"
     CDS             complement(<1..285)
                     /locus_tag="M2A35_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="negative regulator GrlR"
                     /protein_id="HCB7201758.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGN"
BASE COUNT          168 a          108 c           70 g          191 t
ORIGIN      
        1 atttcctctt acatggcaat gtaatgtgac attactatca ttactcagta cttttttagg
       61 gataactaat tgataatctt gttcaatgtt tagcaccgag ggaatttcat gattatagcg
      121 atggacatga agtatgatgt cctcctcttc agacaggacc ccctgataca cagaagcaat
      181 atctccgcca gtgatcaaat ttccattttt aatcagtata ccttccccac aggaatcttc
      241 attgctaata aagataatgc tatagatgcc atccttcata atcataactc actcagtttc
      301 atactaacct cactcctttc aatttgttct atccataagc gatatcaaga ataacggaga
      361 cctttctttt tctccagatt gcaatgtaat atatcattaa atatactcat attccaacct
      421 gttgttttcc gtggttgaca tatttaattt ctgatgaatc atattgaata tcaacatgaa
      481 aatgaaaaaa attatattga gtatcattct catttcaaat tcttgttatg cttctga
//
LOCUS       DAHBHC010000394          535 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.399, whole genome shotgun sequence.
ACCESSION   DAHBHC010000394 DAHBHC010000000
VERSION     DAHBHC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.399"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
BASE COUNT          157 a          107 c           96 g          175 t
ORIGIN      
        1 cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgcg ccatcgtagt
       61 tctccgctgg cgcagtagaa tgggtgttca gcccgttatg tagtatacca gaattaatgc
      121 caatactaac aggatgctct gactcgcaat tcatccagca gtttatcatt tcccataatg
      181 tcacttaccc tcatcggtaa aaaaattgcc tttcgaccat tacgatacat cattgatttt
      241 gggatttcag ggaagtaatc catttcgact ataactgaca ggtcatcacg acgtatgact
      301 gcgtatttgc tactaaatag tttctttatt ttttccacga tgcctccagg tttataagta
      361 caaaaggtta tatccacata gagacaaaaa tattaatctg aaaaatattt atttcacgcc
      421 atatatttga ttatttaatg tgcaggtaca atgactttta ttttgtgttg tgtatataat
      481 caaatatatg gttatttttc accctgcgta ttcagcgcgc aacaaaaaac ccgcc
//
LOCUS       DAHBHC010000395          534 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHBHC010000395 DAHBHC010000000
VERSION     DAHBHC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.400"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..261
                     /gene="escR"
                     /locus_tag="M2A35_005643"
     CDS             <1..261
                     /gene="escR"
                     /locus_tag="M2A35_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HCB7201759.1"
                     /translation="NETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIIS
                     NILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            261..530
                     /gene="escS"
                     /locus_tag="M2A35_005644"
     CDS             261..530
                     /gene="escS"
                     /locus_tag="M2A35_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HCB7201760.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
BASE COUNT          144 a           94 c          104 g          192 t
ORIGIN      
        1 aatgaaacta tcttaaaaaa agactctcta tttatactgt taccagcttt cacgatgggg
       61 cagcttgaag ctgcattcaa gataggtttt ttgctctatt taccttttat tgcgatagat
      121 ttgatcattt ccaatatctt attggccttg ggtatgatga tggtatcgcc agtaacaatt
      181 tcgattcctt ttaagatctt gttattcatt cttgttggag gctggcaaaa attattcgaa
      241 tttctgttgg tggtgaattg atggaaactg gatattttgt tcaattatgt gtgcaaacgc
      301 tttggataat atttatcctc tcactgccta cagtcatagc ggcctctgtt gtcggcatta
      361 ttattagcct ggttcaggct ataacgcagt tacaggatca aacattgcct tttttgctaa
      421 aaataatagc agtgtttgct acgcttgccc tgacttatca ctggatggga acaacaatca
      481 tcaacttctc gtcgataata tttgaaatga ttccgaaggt gaatggctaa tgaa
//
LOCUS       DAHBHC010000396          520 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHBHC010000396 DAHBHC010000000
VERSION     DAHBHC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.401"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(12..>520)
                     /locus_tag="M2A35_005645"
     CDS             complement(12..>520)
                     /locus_tag="M2A35_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201761.1"
                     /translation="GVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELSDINKEG
                     FLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQGGQCK
                     PEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPKSGEIKPTHINDLFLNRLNK
                     ILLPYETL"
BASE COUNT          167 a           96 c           71 g          186 t
ORIGIN      
        1 tatttttata ttcataaagt ttcataaggc aataaaattt tattaagtct gttaagaaat
       61 aaatcattaa tatgggttgg ttttatttct ccacttttcg gtaaaagata tttgactata
      121 gtccataagg gattatcaga aaatagccat tcaagatttc ctgcaccgta ttttatcagt
      181 tcagaacaaa accgttcttc gccatattct ggtttacatt gccctccctg ccaatagaat
      241 gataactgct tcacattttg atatcctatc tctctgatca gagtttctcg atataatcgt
      301 gaaaaaatat agtcagggca ttgaatttca aataatgcac tacttagagc aagcaatctt
      361 attttcccaa ttgactgata taaaaaacct tccttattta tatcagataa ttccactcca
      421 gaaattttat cttgtatttc ctgacgcaat atcttgttga gttgattacg agtagaacta
      481 ctttctctta aatgccgaaa actaacctga ttgacaccgg
//
LOCUS       DAHBHC010000397          515 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.402, whole genome shotgun sequence.
ACCESSION   DAHBHC010000397 DAHBHC010000000
VERSION     DAHBHC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.402"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..314)
                     /locus_tag="M2A35_005646"
     CDS             complement(<1..314)
                     /locus_tag="M2A35_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCB7201762.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTGFCIF
                     AL"
BASE COUNT          153 a          131 c           78 g          153 t
ORIGIN      
        1 ctgagggcaa aaatacagaa tccggtgtca ataagcttac cttcggttac ctgattttta
       61 agtttttgag ctacggcctg ttcttctgtc agttcagctc tggcccgaag cagcctttcc
      121 tccagatcgc cctcgtcatc aggtattctc tgattgtgtt gtcgccgctc gcgatctatc
      181 tccagtacag ttttaacgtc ataaaaaact tccctccccc gacgctcaac aggaggaacg
      241 ccccatttat caaatgcctg aacagatata ccgatggagg aggccatatc acttttattc
      301 aataaaaagg ccatctcctc tccataagcc atcgataaaa agcgatacaa caaccatgtg
      361 tttttacaaa accatttgat atcattgaca tttttcgcat tgacgacatc aaaacacatc
      421 gtaaggttgt tgtatttatt ttattttcac cttacttatc aattagatat accaaacaat
      481 taaacaacaa ctccccctca aaaaatctca taaat
//
LOCUS       DAHBHC010000398          512 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHBHC010000398 DAHBHC010000000
VERSION     DAHBHC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.403"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(141..>512)
                     /locus_tag="M2A35_005647"
     CDS             complement(141..>512)
                     /locus_tag="M2A35_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201763.1"
                     /translation="TNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECMASFYGP
                     AGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDMTVRLRR
                     KVVREYGIKSLVEAPVIFFGD"
BASE COUNT          138 a          151 c          119 g          104 t
ORIGIN      
        1 cgaatgcgct cacttaccgg aatgattgtg gacgggccga gaatgagtaa tgcaccaaaa
       61 tttcgccctg atgctgcacg cggcgacatg atcacatcaa cattaacaac gtttgataca
      121 ggcaagccct gtgccatagc ttaatctccg aaaaagatga ctggtgcttc caccagcgat
      181 ttaataccgt actcgcgcac aaccttccgg cgcagacgca ccgtcatatc gtagcggcgg
      241 acccattgct gattaataag ttcagggaag ggagtcagac ctgtgtaatc gccaagagac
      301 agccccagcg cattcagtgc tgcgttgttc tgcggtacag atataccgtc acgaaaccgg
      361 gacgcataca ccatccccgc cggaccataa aacgaagcca tacactcaat cgtttcatgc
      421 cgccagagct gagagccatc atcggtctgt ctggtgaatg ccggactgtc atcacctgac
      481 catccgataa ccccaaacgc acaccagttc gt
//
LOCUS       DAHBHC010000399          512 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHBHC010000399 DAHBHC010000000
VERSION     DAHBHC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.404"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..503
                     /locus_tag="M2A35_005648"
                     /pseudo
     CDS             1..503
                     /locus_tag="M2A35_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          185 a           77 c          103 g          147 t
ORIGIN      
        1 cggagtgtaa caaatctaca gcctaaatat ttattttagg agaatttgca ggtggaatga
       61 tggggtccga actcgtaaaa tgggttaaat cgcataagat agatgctcat attattacat
      121 ttgtagcaaa gatgccgtat attgattcaa taaaattgct tgaagcaggt gcgaaaggat
      181 gcgtatggaa aaccagtcac ccggcgaaac taaatcgtgc tattgattcg attagtaatg
      241 gttacactta ttttgatagt gtacatatgg attgtgaaaa aatatcttcc aggtattcct
      301 ctgataatca acttacaaat cgtgagtcag aaatattacc actcatagct gatggtaaaa
      361 caaacaaaga aatcgctaat ttcttgcagt taagcagaaa aacagtcgaa actcatagac
      421 tcaatatcat gaagaaatta gatgttcaca gtggtatcga gttgatcaaa acagctttac
      481 gtatgggtgt ttgcactata taaaaaaaag ag
//
LOCUS       DAHBHC010000400          508 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.405, whole genome shotgun sequence.
ACCESSION   DAHBHC010000400 DAHBHC010000000
VERSION     DAHBHC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.405"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>508
                     /locus_tag="M2A35_005649"
     CDS             <1..>508
                     /locus_tag="M2A35_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCB7201764.1"
                     /translation="QPSRLAWLLQPPGKINGKNVLQHIDRLNSITALELPDGIELSVH
                     QNKLLKLAREGRKMSSRDLAKFTDIRRYATLVCVIQEARATLTDEVIELHERIMGGLF
                     SQAKHKQAERLQLTGKLIQSKLRQYVTVGQALLHARKSGEDPWAAIEDVIPWQEFVNS
                     LEETQLLSR"
BASE COUNT          148 a          123 c          123 g          114 t
ORIGIN      
        1 acaaccttcc cgacttgcgt ggctacttca gcctcccggt aaaattaacg gtaaaaacgt
       61 gctgcaacat atcgatcgac ttaattctat caccgcgctg gaattacctg atggtattga
      121 gctttccgtt caccagaaca aattactcaa actggcgcgt gagggccgga aaatgagcag
      181 cagagatctg gcaaaattca ccgatatcag acgttacgct acgctggttt gtgtcattca
      241 ggaagccaga gcaacattga ctgatgaagt tatcgagcta cacgaacgca ttatgggcgg
      301 tctgttcagc caggcaaaac acaaacaagc cgagaggctt cagttaacag gcaaacttat
      361 ccagagcaag ttaagacaat atgttactgt cggtcaggcg ctactgcatg ccagaaagtc
      421 aggtgaagat ccctgggccg cgatagaaga tgttattccc tggcaggagt tcgtcaacag
      481 cctggaagaa acacagttgc tgtcccgt
//
LOCUS       DAHBHC010000401          503 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.406, whole genome shotgun sequence.
ACCESSION   DAHBHC010000401 DAHBHC010000000
VERSION     DAHBHC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.406"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..75
                     /locus_tag="M2A35_005650"
     CDS             <1..75
                     /locus_tag="M2A35_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCB7201765.1"
                     /translation="DADSNLQSLCWPCHKAKTARERIN"
     gene            223..>503
                     /locus_tag="M2A35_005651"
     CDS             223..>503
                     /locus_tag="M2A35_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCB7201766.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDPKKNAKKDEKGLPKIPQHL
                     GSQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEG"
BASE COUNT          141 a          131 c          128 g          103 t
ORIGIN      
        1 gatgcagaca gtaacctgca gagtctgtgc tggccctgtc ataaagcaaa aacagcgcgc
       61 gaacgcatca attgataaca gctcccatct gtaggggagg ggcgggtcaa atctctgcaa
      121 ccctggctgc tcagtaccgc cgcctgaccc ttcctcgcat cgccgcaggt tcgaaaactt
      181 ttttttggaa tgtgattaaa tgattgatag gtaaaaccga ttatgtccgg acccccgaaa
      241 accccgccac gcctgcattt gatacgaggc aacccctcaa agcgccccgt taaagacccc
      301 aaaaaaaacg ctaaaaagga tgaaaaaggt ctccctaaaa ttccgcaaca tttagggtcg
      361 caggggaagt actggttcag gcgaatggcg gaagagctga atgcggaagg gatcatttct
      421 cagcttgatg cacgtgcact cgagttgctg gtggaagcct ataccgaata ccggcatcac
      481 tgcgaaacac tcgatgttga ggg
//
LOCUS       DAHBHC010000402          500 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHBHC010000402 DAHBHC010000000
VERSION     DAHBHC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.407"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..222)
                     /locus_tag="M2A35_005652"
     CDS             complement(<1..222)
                     /locus_tag="M2A35_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCB7201767.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASE"
BASE COUNT          140 a          140 c          101 g          119 t
ORIGIN      
        1 ctcgctggcc tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc
       61 atccctttcg gcataccatt ttataacggc ggcagagtca taaagcacct cattaccctt
      121 gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact
      181 cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgctcatt ccacggacaa
      241 aaacagagaa aggaaacgac agaggccaaa aagctcgctt tcagcacctg tcgtttcctt
      301 tcttttcagg gggtatttta aataaaaaca ttaagttacg acgaagaaga acggaaacac
      361 cttaaaccgg aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaacctg
      421 tcggatcacc ggaaaggacc cgtaaagtga taatgattat catctacata tcacaacgtg
      481 cgtggaggcc atcaaaccac
//
LOCUS       DAHBHC010000403          500 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHBHC010000403 DAHBHC010000000
VERSION     DAHBHC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.408"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>500)
                     /locus_tag="M2A35_005653"
     CDS             complement(<1..>500)
                     /locus_tag="M2A35_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCB7201768.1"
                     /translation="DELQIWDALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGK
                     GKFKGILPLDRWMIDPVLTRRIKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFD
                     GVTLPFQQKMTENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELI
                     ALGGPA"
BASE COUNT          111 a          155 c          127 g          107 t
ORIGIN      
        1 tacgcaggac caccaagtgc gataagctcg cgtagcttct ccacgctgta ggtacgcaaa
       61 tgcgctttgt agaccagctg cgccgcgccg acagtagcgc tgtcgaacgc ggtaagacga
      121 tcccagatac gctctacaac cgacattccc cattcgttct cggtcatctt ctgctgaaat
      181 ggcagcgtga cgccatcaaa gcgaatcagt cgactgtgat gaatgcgcca ggcaggaatt
      241 cccgttgctg tggtcaccac atcgtaaaac tcaggtttac ccaggtccgg ccccatatct
      301 ttaatgcggc gggtcagtac cgggtcaatc atccagcggt cgagcgggag aatcccctta
      361 aacttgccct taccgatggt ttcgggtcgc agcggggtca ttggtgcctg cccctcaatc
      421 atgatgaaac ccaccgcgcc gccgtagagg cgcgaccatt tcagcacgtc attcagcgca
      481 tcccagattt gcaactcatc
//
LOCUS       DAHBHC010000404          495 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.409, whole genome shotgun sequence.
ACCESSION   DAHBHC010000404 DAHBHC010000000
VERSION     DAHBHC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.409"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>495)
                     /locus_tag="M2A35_005654"
     CDS             complement(<1..>495)
                     /locus_tag="M2A35_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201769.1"
                     /translation="GIASLNDRYSARSFCDAVSRAACWQKATRLRLFQFFGVPSVTAY
                     RQMRSFRAPLEAHHINMQQFTPQQVAELEVIRMACDTGDFRTYILLNGGFFCSERLLR
                     PFYVQPQEGGKPRINRYGEPCAPVISGFMFGPVPVITRFMGCVVRRMTPAEKIRADEI
                     RSGSS"
BASE COUNT          129 a          132 c          147 g           87 t
ORIGIN      
        1 actgctgccg cttctgattt catctgcgcg gattttctct gcgggggtca tccggcggac
       61 aacgcagccc ataaaacggg ttatgaccgg aacggggcca aacataaaac cggaaatcac
      121 cggcgcgcag ggttcaccgt aacggttaat acggggttta ccgccttcct gcggctgaac
      181 gtaaaacgga cgaagcagac gttcagaaca gaagaaaccg ccattcagca aaatgtaggt
      241 acgaaaatca cccgtatcac aggccatgcg gatgacctcc agttcggcga cctgctgcgg
      301 cgtgaactgc tgcatattga tatgatgtgc ctcaagcggt gcgcggaagc tgcgcatctg
      361 gcggtaagcc gtgacggacg gaacgccaaa gaactggaaa aggcgaaggc gcgttgcttt
      421 ctgccagcag gcggcacggc tgaccgcgtc acagaatgaa cgggcagaat aacggtcatt
      481 cagggaggca atacc
//
LOCUS       DAHBHC010000405          479 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHBHC010000405 DAHBHC010000000
VERSION     DAHBHC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.410"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(83..274)
                     /locus_tag="M2A35_005655"
     CDS             complement(83..274)
                     /locus_tag="M2A35_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCB7201770.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(271..459)
                     /locus_tag="M2A35_005656"
     CDS             complement(271..459)
                     /locus_tag="M2A35_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCB7201771.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
BASE COUNT          138 a          101 c           92 g          148 t
ORIGIN      
        1 ctcattacag atttaagggt gaacaaatcc ctgccattgc tggcatataa gaatgaaatc
       61 ggatgtttat tacggaactg ttttaaagac ctgccgggat ttcgttatta tcctggtgaa
      121 taactttatc gaccgggtaa cagttaccgg gaattttctg ttcggttgct gcagtcacac
      181 actcctgcat tgtcctgtga acactgactg caatatcaac tggctctccg gaaacaagaa
      241 aaactgtcag aacaagtgca aatgctgtat tcattgccag catccttttt gtatcggacg
      301 taaacgggcc aacattgaaa gaatgcatat tttatttaat agctcccgtt cgtgttttct
      361 cttgttaatg gcatcttcag taaatacagg gttactgata gtgacaccaa tttcaaaaca
      421 accttcagac gtattaacgt ttggtaataa cgtttccatt atcgcgtcct caacaatga
//
LOCUS       DAHBHC010000406          479 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.411, whole genome shotgun sequence.
ACCESSION   DAHBHC010000406 DAHBHC010000000
VERSION     DAHBHC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.411"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(275..>479)
                     /locus_tag="M2A35_005657"
     CDS             complement(275..>479)
                     /locus_tag="M2A35_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCB7201772.1"
                     /translation="LICILRQRYEGRGMTKRKMAELLNDSHPEWCYATCRNRIDAWLK
                     MAEFMLYLPMRDAFSSGDLKTVC"
BASE COUNT          121 a          139 c          112 g          107 t
ORIGIN      
        1 tcataatgac gccctctggt ggtttcttaa taactcacca ccgacgacgc caatcgtctg
       61 gtggtgaact gtgcagggtt ggcgtaaccg ggaaaccgac cggcgcggat ctctccgccc
      121 ccacacagcc caccataatt cagatgtgcg cgtgcatacg acaataaaaa acacgctcgc
      181 ggcgtgtatc tgtcgcggtc tctatccagg acgccaatcc cgacgccaga ttttgctggc
      241 gcgtgaggaa tatagccccg gataacagat tgagtcaaca gacggttttt agatccccgg
      301 aagagaatgc atcacgcatc ggcagataga gcataaactc tgccattttc aaccacgcat
      361 ctatgcgatt acggcacgtg gcgtaacacc actcagggtg tgaatcattc agcaattcag
      421 ccatttttcg cttagtcatc ccccgccctt catatcgttg ccggaggata caaatcaat
//
LOCUS       DAHBHC010000407          476 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.412, whole genome shotgun sequence.
ACCESSION   DAHBHC010000407 DAHBHC010000000
VERSION     DAHBHC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.412"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(5..145)
                     /locus_tag="M2A35_005658"
     CDS             complement(5..145)
                     /locus_tag="M2A35_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCB7201773.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPQ
                     NG"
BASE COUNT          133 a          105 c           93 g          145 t
ORIGIN      
        1 tgttttatcc attttgggga actcctggct gattaagtac gtcgatgagt cgtttccatc
       61 cgtcacgtaa tttacgtgtg attcgctcaa gtaaagattc ggaagggcag ccagcaacag
      121 gccaccctgc aatggcatat tgcatggtgt gctccttatt tacgctttaa attgctccgt
      181 gtattcactc gttggaatga atacacagtg cagtgtttat tctgttgttt atgccaaaaa
      241 taaaggccga ctatgcggcc tgaaattact taaccaatga tgctgcatat tcgataaggt
      301 aaagctttgg ggccagccaa atttttaacc aagtcatatt ggttactgca ccaataataa
      361 aaatccccca cagagtcaaa actccaacca atggcatgat aagaagatta atatcacctt
      421 tgctatccca aaccattgtc ggcctgtatt tgggatttcc tttctcccat gaatat
//
LOCUS       DAHBHC010000408          471 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.413, whole genome shotgun sequence.
ACCESSION   DAHBHC010000408 DAHBHC010000000
VERSION     DAHBHC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.413"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>471)
                     /gene="tssI"
                     /locus_tag="M2A35_005659"
     CDS             complement(<1..>471)
                     /gene="tssI"
                     /locus_tag="M2A35_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCB7201774.1"
                     /translation="RFKSAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGRRIVLTG
                     HPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLV
                     DGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAGTG"
BASE COUNT           65 a          174 c          127 g          105 t
ORIGIN      
        1 aaccggtgcc tgcccacgcc tgtgccacac ggatccagca cgaactgtct tggtctgccg
       61 ggttataacg gtcccagttg aacttcaccc gcacgcgacc atgttcgtcg cagaagattt
      121 cctcgcctgc cggtcctgtc acgacagcgc tctgcgggcc gtcgaccagc ggtttcagca
      181 acggctgtgg tcgccatgtt ctgtctgccg ggatcaccgc aaaatggttc tccagcgccg
      241 tccccgctcc ctgccgtcct ggcaccgcct gtggctgttc gccgtgcagt tcacttgcca
      301 ccacctgcca ttcccggttc aggttcgcct gcggatgccc cgtcagcaca attcgccgtc
      361 ccggccatat ctccggcgag cggctcattc cccgcgcggt ttctgcgttg tttcgccagc
      421 cgtccatctg ccagcgggca aagttctgcc cgtgggcgct cttgaaacgt c
//
LOCUS       DAHBHC010000409          471 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHBHC010000409 DAHBHC010000000
VERSION     DAHBHC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.414"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..163)
                     /locus_tag="M2A35_005660"
     CDS             complement(<1..163)
                     /locus_tag="M2A35_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201775.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKY"
     gene            complement(160..372)
                     /locus_tag="M2A35_005661"
     CDS             complement(160..372)
                     /locus_tag="M2A35_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201776.1"
                     /translation="MLFLNDKEQIIKYKDEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
BASE COUNT          159 a           83 c           78 g          151 t
ORIGIN      
        1 catatttagc gccagtgctg gtgatcaagt ctccgcattc aacatcagca ggaagagaag
       61 caaaaacctt gctttctaca gaggcaaacg gatcagtaca ataacttaca atatctgtga
      121 aagtctttcg aactggaaat agagtgtcta aatcaataat catttagtaa tctccttagc
      181 ttttaatttg attaattcat taagggaagg tgtagacgga gaagaaggag aaaaatattt
      241 ttcaacacat ctttctactt cctcgacaga acacccagcg tatttagcta ccatttcggt
      301 aatttgtgga ttaattttca gcatttcatc tttatacttg atgatttgct ctttatcgtt
      361 taaaaacaac atttatataa accttgttac cttgtttaaa gtaagaaata caacttttaa
      421 gttgcataac gggcaaaata aaaggcagca tttagagccg ccttttatta a
//
LOCUS       DAHBHC010000410          466 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHBHC010000410 DAHBHC010000000
VERSION     DAHBHC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.415"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            150..>466
                     /locus_tag="M2A35_005662"
     CDS             150..>466
                     /locus_tag="M2A35_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCB7201777.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FAL"
BASE COUNT          139 a           88 c          127 g          112 t
ORIGIN      
        1 atccatgtcc ggtttctctg caagttaact atatgaaaaa tataaaaaca ggctgtccgt
       61 gaaccggaca tgtgcaaaaa acggacatgt aaaccggaca tgaccggttt tgtgctgatt
      121 gtgaggtaag agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt
      181 tgagtctgac cgatcgccag cttcgcagat tgcagaaatt accgggtgcc ccggtctcga
      241 ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagaa
      301 gaagtaaaaa cgatgtgcct gatggcgata gcgaagaaga ctatgaagag aaattgctta
      361 ttgccagatg ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt
      421 caaaaggaaa actgattgac accgggttct gtatttttgc cctcag
//
LOCUS       DAHBHC010000411          461 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.416, whole genome shotgun sequence.
ACCESSION   DAHBHC010000411 DAHBHC010000000
VERSION     DAHBHC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.416"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            109..>461
                     /locus_tag="M2A35_005663"
     CDS             109..>461
                     /locus_tag="M2A35_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCB7201778.1"
                     /translation="MTPDIIQQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSP"
BASE COUNT          124 a          124 c          122 g           91 t
ORIGIN      
        1 ccagatattt cgccgcgaca ttcgcgcatc gtcagaactg acacaggccg aagcagtgaa
       61 agctcttgga ttcctgaaac agaaagccac tgagcagaag gtggcagcat gacaccggac
      121 attatccagc agcgtaccgg gatcgacgtg agagctgtcg aacaggggga tgatgcgtgg
      181 cacaaattac ggctcggcgt catcacagct tcagaagttc ataacgtaat agcaaaaccc
      241 cgctccggaa aaaagtggcc tgacatgaaa atgtcctact tccacaccct gcttgctgag
      301 gtttgcaccg gtgtggctcc ggaagttaac gctaaagcgc tggcctgggg aaaacagtac
      361 gagaacgacg ccagagccct gtttgagttc acttccggcg tgaatgttac tgaatcaccg
      421 atcatctatc gcgacgaaag tatgcgtacc gcctgctctc c
//
LOCUS       DAHBHC010000412          461 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.417, whole genome shotgun sequence.
ACCESSION   DAHBHC010000412 DAHBHC010000000
VERSION     DAHBHC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.417"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>461
                     /locus_tag="M2A35_005664"
     CDS             <1..>461
                     /locus_tag="M2A35_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system"
                     /protein_id="HCB7201779.1"
                     /translation="VVTGYIYEVQTGRSEGSGLALAAKLLSGRFGVSVGSSSSMGNYI
                     SFSSGTLNAMYELFSTDNRFKVVSAPQLRMDSGKEATFSVGEQVPVLGSVSYEDGKAV
                     QSVTYRDSGVIFKVKPVITSSRISLNVNQQLSNFVKTDTGVNDSPTLLKREV"
BASE COUNT           99 a           94 c          143 g          125 t
ORIGIN      
        1 gtggtgacgg ggtatattta tgaggtacag acaggccgtt ctgaaggttc ggggctggcg
       61 ctggccgcaa aactgttatc aggtcgtttt ggggtcagcg tgggttcgtc gtcatcgatg
      121 ggtaattata tttcgttcag ctccggtacg ctgaatgcga tgtatgagct gttcagtacg
      181 gataaccgct ttaaggtggt atccgcacct cagttacgca tggattcagg caaggaggcg
      241 acgttctccg ttggtgaaca ggttccggtt cttggcagcg tgtcctatga ggatggtaaa
      301 gccgttcagt cggtgacgta tcgtgattcg ggggtgatat tcaaagttaa gccggttatc
      361 acgtcgtcac gcatcagcct gaacgtcaat cagcagttaa gtaactttgt caaaacggat
      421 acgggcgtga atgattcgcc aacgttgctc aagcgtgagg t
//
LOCUS       DAHBHC010000413          456 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.418, whole genome shotgun sequence.
ACCESSION   DAHBHC010000413 DAHBHC010000000
VERSION     DAHBHC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.418"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..258)
                     /locus_tag="M2A35_005665"
     CDS             complement(<1..258)
                     /locus_tag="M2A35_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201780.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTF"
BASE COUNT          165 a           92 c           62 g          137 t
ORIGIN      
        1 gaatgttact accagatcag ggtcggtagt aattattatc tcaaaactac ctgtaagctc
       61 gccataagga acgactattg tgttttctgg taactcagaa aaagcatcac ttcttcctaa
      121 cgaaacatca ttaccaatga taacaagtag acgcccatta agtatttcat actcgcatcc
      181 attcaatggt ccgctcaaca aacgaatggc ataagattcc aataagtctt gtgataagaa
      241 tttatcatta ttttccatga ataccacccg ctaattccat aagctctagt ttagagtagt
      301 gtaacaagac ggaaatttca tgaccaaatt atgatgacac agaataaaaa aattataatt
      361 tcgagaacta ttttaaatgc ctctatgcat aactttattt acaatttaag gtataaaccc
      421 tattccctca atgttaagca aatataacaa tcactc
//
LOCUS       DAHBHC010000414          454 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.419, whole genome shotgun sequence.
ACCESSION   DAHBHC010000414 DAHBHC010000000
VERSION     DAHBHC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.419"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..391)
                     /locus_tag="M2A35_005666"
     CDS             complement(<1..391)
                     /locus_tag="M2A35_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCB7201781.1"
                     /translation="MILGNDYVDLAPLFSAHSTRNYLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEYLIEIPYFLRQDLIKPQDVQGKRKPGTD
                     FQETLTDIYADYVAKHHVAYQRTKESVL"
BASE COUNT          134 a           86 c           98 g          136 t
ORIGIN      
        1 ccaatacgct ttctttagtg cgctgatacg ctacgtggtg tttagcaaca tagtcagcat
       61 agatatcagt gagtgtttcc tgaaaatcag tgccaggctt acgcttgcct tgcacgtcct
      121 gcggtttgat caggtcttgc cttaaaaagt acgggatttc gataagatat tctttgcctt
      181 gttcacgctt agtaacgttg tgttcagaag agaaacgaga ggtttctttg ttgaacagag
      241 attcgttgct ttcgacaagc tgggaaacag ctactttatg agatgtaacc cctacagaat
      301 ccgtaaaatc aagagtagaa agcagatagt ttctagtgct atgcgcagag aacagaggag
      361 ctaaatcaac gtaatcatta cctaaaatca tttattatta ttccttttat taagaagtga
      421 aaacagaatg gaacccttca agctggaagc cttt
//
LOCUS       DAHBHC010000415          454 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHBHC010000415 DAHBHC010000000
VERSION     DAHBHC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.420"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..454
                     /locus_tag="M2A35_005667"
                     /pseudo
     CDS             1..454
                     /locus_tag="M2A35_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          132 a           82 c          109 g          131 t
ORIGIN      
        1 tgttgactat ttgtgccgtt atttctggtg cagaaggctg ggaagatata gaggattttg
       61 gggaaacaca tcccgatgtt ttgaagtgat atggtgattt tgaaaatggt attcctgttc
      121 acgatatcat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac gagtgcttta
      181 ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcattgca attgatggaa
      241 aaacgctccg gcactcttat gacaagagtc gtcgcagggg agcgattcat gtcattagtg
      301 cgttctcaac aatgcatagt ctggtcctcg gacagatcaa gacggatgag aaatccaatg
      361 agattacagc cattcctgaa cttcttaaca tgatggatat taaaggaaaa atcatcacaa
      421 ctgatgcgat gggctgccag aaagatattg caga
//
LOCUS       DAHBHC010000416          447 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.421, whole genome shotgun sequence.
ACCESSION   DAHBHC010000416 DAHBHC010000000
VERSION     DAHBHC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.421"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>447)
                     /gene="bluF"
                     /locus_tag="M2A35_005668"
     CDS             complement(<1..>447)
                     /gene="bluF"
                     /locus_tag="M2A35_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCB7201782.1"
                     /translation="GSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRF
                     GKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIP
                     AEDSWLFIADGSDKELDSCALSPTINDHFAFHPIVDPLSRRIIAFEA"
BASE COUNT          134 a           95 c          108 g          110 t
ORIGIN      
        1 ggcttcaaaa gcgattatcc gccgcgataa gggatcgaca ataggatgaa aggcaaagtg
       61 gtcgtttata gttggtgaaa gggcacagga atcaagttct ttatcagatc cgtcagcgat
      121 aaaaagccag gagtcttcgg cagggatctc gaaataggtt gattgttcgg ttgcaaggac
      181 aaaagtacga aaaaattgta gcgctctgtc atcataagtt agctgaaatt ttgatgtgcc
      241 tttgtcgaat acggcctgta aaacgtcatc tcgctcgtgc aggcgcaaat caaataattc
      301 cattcccgct ttgccaaaac ggcgagcagg cgcgtaatcg cacagcagct caacaatatt
      361 atagtgccgt ggatcctggc atatagcccg atatatcatt ttaacctgtt cttccggacc
      421 ttccagaagc tggaaaaaat gagaacc
//
LOCUS       DAHBHC010000417          443 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.422, whole genome shotgun sequence.
ACCESSION   DAHBHC010000417 DAHBHC010000000
VERSION     DAHBHC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.422"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..310)
                     /locus_tag="M2A35_005669"
     CDS             complement(<1..310)
                     /locus_tag="M2A35_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCB7201783.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     C"
BASE COUNT          117 a           93 c           93 g          140 t
ORIGIN      
        1 cacaccattc attattcaat aaaatgccat tcgtttgtaa tgcattaaaa atacgttttt
       61 ggcctgcata gcgttgttga tagtgaataa ctttacggaa aaaatccagg ccagccagag
      121 tgggttcacc gccttgccag gtaaaataga cctgattgcc agacgctgcg atatattgtt
      181 tgatgaactc tttcagagta ctgtcatcca tccatttttc atgagtaaac tgcgactctt
      241 tttcaaggta aaaacagtaa tcacatttga gattacattg aaaactggag ggcttggctg
      301 taacgtgcat cgctatctcg ctcaataagg cggcggaaaa ttccgccgca tgaaggttta
      361 gttatttcgc ttcgcttagt gctttcttga tattgttaaa cttctcctga tttacctcgc
      421 taagcggtgg ctggctgctg tcg
//
LOCUS       DAHBHC010000418          435 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.423, whole genome shotgun sequence.
ACCESSION   DAHBHC010000418 DAHBHC010000000
VERSION     DAHBHC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.423"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..349
                     /gene="bluF"
                     /locus_tag="M2A35_005670"
     CDS             <1..349
                     /gene="bluF"
                     /locus_tag="M2A35_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCB7201784.1"
                     /translation="AEAIKSLKAAAISVAIDHFGAGFAGLLLLSRFQPDRIKISQELI
                     TNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWIWLESAGIEMFQGDLFAKAK
                     LNGIPSIAWPEKK"
BASE COUNT          135 a           80 c           93 g          127 t
ORIGIN      
        1 tgccgaagcg attaaatcgc taaaggctgc tgctatcagt gtagcaattg atcattttgg
       61 cgcaggtttt gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag
      121 tcaggaattg attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat
      181 cataaaatgc tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga
      241 agagtggatt tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa
      301 agctaaattg aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca
      361 ttctttcaca tgatttcagt aaatcatcgt gaaaatgaat acgttacgtt taaatttaat
      421 ccacattaaa aataa
//
LOCUS       DAHBHC010000419          433 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.424, whole genome shotgun sequence.
ACCESSION   DAHBHC010000419 DAHBHC010000000
VERSION     DAHBHC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.424"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..239)
                     /locus_tag="M2A35_005671"
     CDS             complement(<1..239)
                     /locus_tag="M2A35_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCB7201785.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKP"
     gene            complement(250..>433)
                     /locus_tag="M2A35_005672"
     CDS             complement(250..>433)
                     /locus_tag="M2A35_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCB7201786.1"
                     /translation="FQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTDIKHYILILKREN
                     GVTWLDNFGETDDEKK"
BASE COUNT          104 a          117 c          106 g          106 t
ORIGIN      
        1 ggctttttgt tgacggtggc caccaccaca ccgccggagg cggtctgctc attattggtc
       61 agggcaccat ccagactgtt cacggcgata atccggtcac cgggaacggt aaacagattc
      121 gggctggtat tgctgatggc aatactggcc tgaccaccgt tcaccattgg caccacggtg
      181 ggggccagcg taccgtttgc ggccatcacg ctgcaggaaa aaaacaggct gccgaatatt
      241 attgccgtat tattttttct catcgtctgt ttccccgaaa ttatccagcc aggtcacccc
      301 gttttcccgc ttcagaataa ggatgtaatg tttgatatca gtgaaaggtt ttgaatcacc
      361 aatccaggtt ttaagcacac cacgaatttc cacacggcca tactgaggcc agacacgaac
      421 actggtctgg aaa
//
LOCUS       DAHBHC010000420          431 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.425, whole genome shotgun sequence.
ACCESSION   DAHBHC010000420 DAHBHC010000000
VERSION     DAHBHC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.425"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..302
                     /locus_tag="M2A35_005673"
     CDS             <1..302
                     /locus_tag="M2A35_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCB7201787.1"
                     /translation="LDNKFDSEKYRNNVRELTPAILAVLPLEYRGHLVEQDSFMARLA
                     EMEKELCEAKQAVILNAPRHQKLKEMSEGIVSMFRVDPDLAGPLMAMVTTMLGAI"
     gene            299..>431
                     /locus_tag="M2A35_005674"
     CDS             299..>431
                     /locus_tag="M2A35_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HCB7201788.1"
                     /translation="MTGSEMAKAGLREQSRLSGANRNALIAEGIMANTAEIFNFPVPD
                     "
BASE COUNT          118 a           97 c          121 g           95 t
ORIGIN      
        1 tcctcgataa caaattcgat tctgaaaaat accgaaataa cgtgcgtgaa ctgaccccgg
       61 caattctggc ggtactaccg ctggaatatc gcggccacct ggttgagcag gatagcttca
      121 tggctcggct ggctgaaatg gaaaaggaac tctgtgaggc aaagcaggcg gtcattctca
      181 acgcaccacg ccaccagaaa ctgaaggaaa tgagtgaagg tattgtgtcg atgtttcgtg
      241 tggacccgga tctggctggt ccattaatgg cgatggttac caccatgctg ggggcaatat
      301 gacaggttca gaaatggcga aagccggtct gcgggaacag agccgacttt caggtgcaaa
      361 tcgtaacgca ctcattgcgg aaggaattat ggcaaacact gctgagatat tcaattttcc
      421 agtgccggat g
//
LOCUS       DAHBHC010000421          430 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.426, whole genome shotgun sequence.
ACCESSION   DAHBHC010000421 DAHBHC010000000
VERSION     DAHBHC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.426"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(5..>430)
                     /locus_tag="M2A35_005675"
     CDS             complement(5..>430)
                     /locus_tag="M2A35_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCB7201789.1"
                     /translation="AMNNHIKTHFKELAARLSSIKGVGAMTVATLLSEVPELGSLSRR
                     EISALIGVAPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKAFYTRLIT
                     AGKVRKVALVACMRKLLTILNAMIRKNEEWRDSYHEFSL"
BASE COUNT          118 a          106 c           82 g          124 t
ORIGIN      
        1 atatttataa tgaaaactcg tggtatgaat ctctccattc ttcattcttt cggatcattg
       61 cattcagaat ggttaacagc ttgcgcatgc aggcaacaag agcaactttc ctcacttttc
      121 cagctgtaat gagtcgcgta taaaaagctt ttattacggg attaaatctg gtcgcgacaa
      181 gcgcagccat ataaagcgca gttcttacgc cagctctccc gccaaaaata gttcgtctac
      241 cacgcattgt tccggagtct ctgtttacag gagccacacc tataagcgca ctaatctctc
      301 gacgtgaaag acttcccagt tcaggtactt cagagagcaa tgtggcaact gtcattgcac
      361 caaccccttt aatgctgctc aaacgagctg caagctcttt gaaatgagtt ttaatgtgat
      421 tattcatcgc
//
LOCUS       DAHBHC010000422          426 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.427, whole genome shotgun sequence.
ACCESSION   DAHBHC010000422 DAHBHC010000000
VERSION     DAHBHC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.427"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>426)
                     /locus_tag="M2A35_005676"
     CDS             complement(<1..>426)
                     /locus_tag="M2A35_005676"
                     /inference="COORDINATES: protein motif:HMM:NF017195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201790.1"
                     /translation="YGQYFGASYSAKPTEEVCLSRPEISNTSLSGVYMDDKGNKYVTI
                     HGCIYEATGDVISCSNDSTHCSATWKPLTVDPSYSDKEDTEDKGDSGDTDDKGNNGNT
                     DGGDNSGGSSGGSTSGGSSGGSTSGGSSGGSTSGGSSGG"
BASE COUNT          129 a          109 c           84 g          104 t
ORIGIN      
        1 ctgccaccgg atgaaccgcc agaagtgctg ccgccggagg aaccgccaga agtgctgccg
       61 ccggaggaac cgccagaagt gctgccaccg gacgaaccgc cggaattatc accaccgtca
      121 gtattaccgt tattcccttt atcgtcagta tctccgctat cacctttatc ttctgtatct
      181 tctttatcag aataagacgg atcaacagtt aaaggtttcc aggttgcgga acaatgggtt
      241 gaatcattgg aacaagaaat aacgtcaccc gttgcctcat aaatacatcc atgaatggtg
      301 acatatttat ttcctttatc atccatataa acacctgaca aagaagtatt actaatctca
      361 ggacgggata aacaaacctc ctcagtaggt ttagctgaat acgaagcacc aaaatattga
      421 ccataa
//
LOCUS       DAHBHC010000423          424 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.428, whole genome shotgun sequence.
ACCESSION   DAHBHC010000423 DAHBHC010000000
VERSION     DAHBHC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.428"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            95..>424
                     /locus_tag="M2A35_005677"
     CDS             95..>424
                     /locus_tag="M2A35_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201791.1"
                     /translation="MKNIDTETQNNTVADDVTADEDMAVERGVKLSRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMS"
BASE COUNT          103 a           93 c          125 g          103 t
ORIGIN      
        1 gcgcccgatc gcatccgttc cgcgcttggg cttggctgat aagtaatatg caggcggcgc
       61 agtgcgtcgc ctctgactga aaaggagacc actgatgaaa aacatcgata ctgaaaccca
      121 gaataacacc gtggcggatg atgtgacggc agatgaggat atggctgtcg aacgtggtgt
      181 aaaactttcc cggccaattg agcgtggcgg cgaaaaaatc acgtatgtgg agatcaccgg
      241 ggctattgag caggctggat ccctgcgagg tttgtcgctg tctgatgtgc tgaatctgaa
      301 agcggatacc atgttcacgc tgttgcctcg cgtgacttcg ccacgactgg atgaagtgat
      361 gattaaaaaa atgtcgtcac gcgattttat tcagttgtgc gctgtggctg taaattttat
      421 gagc
//
LOCUS       DAHBHC010000424          423 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.429, whole genome shotgun sequence.
ACCESSION   DAHBHC010000424 DAHBHC010000000
VERSION     DAHBHC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.429"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>423)
                     /locus_tag="M2A35_005678"
     CDS             complement(<1..>423)
                     /locus_tag="M2A35_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCB7201792.1"
                     /translation="QTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHKVDGNPYS
                     HLPDDVRETLQYRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAIDAGLADEL
                     VNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATA"
BASE COUNT           82 a          126 c          115 g          100 t
ORIGIN      
        1 agcagtggct gaaacagttg ttgattgagt ctctttggtc attcgccctc ctgagagacg
       61 ggatttacgt gcatccagtg catcacgcat gacggtgatc gcatcggtgc tgttaacaag
      121 ttcatcagcc agtccggcat caatggcctc ctgaccgctg tacactgcag cctcggtatc
      181 cagcacagcc tgcacggaca ggccggtata tgccgacacc ttctgcgcaa acatccggcg
      241 ggttgcgtcc atccggtact gcagtgtctc ccggacgtca tccggaagat ggctgtaggg
      301 gttgccatcc accttatggc tgccgctgta aatcagcgtg atttccacac cctgtttctc
      361 cagcgcagca ccgtaattac tgtgagccat catgacgccg atggagcctg tccgggcggt
      421 ctg
//
LOCUS       DAHBHC010000425          423 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.430, whole genome shotgun sequence.
ACCESSION   DAHBHC010000425 DAHBHC010000000
VERSION     DAHBHC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.430"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            1..264
                     /locus_tag="M2A35_005679"
                     /pseudo
     CDS             1..264
                     /locus_tag="M2A35_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F7-2 fimbrial protein"
BASE COUNT          130 a           80 c           93 g          120 t
ORIGIN      
        1 tttttaatct tttggggggt taaattccta ttgatcaagt tagttaactg cgatgccaca
       61 gaaatcggaa aactgaccaa tggtgtgaaa gtttctttta ctggtacaac aatcaatgga
      121 cagactcagg agttaggtac tactggtgat acagggactg caatagttgt ctcagctgcg
      181 aatggcagcc tggtttcatt tgatggtaca gcaggttccg cgacaaaagt taaagaaggt
      241 gataataccc tgcgctacag tacttgaacg ataggaatta aaacccccaa aaggttaaaa
      301 aaacaccaca aaacagatgt ttcttcaaca ccacttttac tccatatgaa aggaatcgac
      361 gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc
      421 cct
//
LOCUS       DAHBHC010000426          416 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.431, whole genome shotgun sequence.
ACCESSION   DAHBHC010000426 DAHBHC010000000
VERSION     DAHBHC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.431"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..243
                     /locus_tag="M2A35_005680"
     CDS             <1..243
                     /locus_tag="M2A35_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCB7201793.1"
                     /translation="NLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNKVSGDIITA
                     RSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            247..>416
                     /locus_tag="M2A35_005681"
     CDS             247..>416
                     /locus_tag="M2A35_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201794.1"
                     /translation="MEFEIKGVKYRVAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKF"
BASE COUNT          118 a           98 c          107 g           93 t
ORIGIN      
        1 aaccttctga agacatcacc gacaaataaa aaattgtcgc tggcgtataa cgcacagagc
       61 cagtcttctg ccacatgggg gaataacgtt atcgtgatcc gcaacaaggt cagcggcgac
      121 atcatcacgg cacgcagtgt tgcgttccag aaacaaccgg ataacgccaa cgctaaaacc
      181 ggtaatacga tgccgtgggt gtttgactgc ggcaagattg accaggttct cggggagttt
      241 taatgcatgg aattcgaaat taaaggcgtg aaatatcgcg tggcaaaact cagcgttttt
      301 gaccaactga aagtgacccg caaacttctg ccggtgttgg caggaatgat gtcagatttc
      361 gggagtattc gctcccgttt gcctgctgac ggcaaaatcg acaccgtgaa attcga
//
LOCUS       DAHBHC010000427          414 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.432, whole genome shotgun sequence.
ACCESSION   DAHBHC010000427 DAHBHC010000000
VERSION     DAHBHC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.432"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..361
                     /locus_tag="M2A35_005682"
     CDS             <1..361
                     /locus_tag="M2A35_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCB7201795.1"
                     /translation="ILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHF
                     HNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRV
                     IIDNILKLKQLTRICMR"
BASE COUNT          153 a           68 c           71 g          122 t
ORIGIN      
        1 aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag tatctcatat
       61 cttatttgaa caggctaaga tacactcaac tgatatagcc tctctctact actatagggc
      121 actagtccac tttcataatg aaaaaattga agaggcaagg atttgtatag acaaatcact
      181 acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt
      241 gcctaacccg ctcaaaaaca acattaaact ctactataaa gaaactgaga gtgaaagcca
      301 tcgagttata attgacaaca ttttgaaatt aaagcagctg acgagaattt gtatgcgata
      361 aatttttatt gacgcaattt tattatttag atgtatgcaa cttgttattt aaac
//
LOCUS       DAHBHC010000428          411 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.433, whole genome shotgun sequence.
ACCESSION   DAHBHC010000428 DAHBHC010000000
VERSION     DAHBHC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.433"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..324
                     /locus_tag="M2A35_005683"
     CDS             <1..324
                     /locus_tag="M2A35_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7201796.1"
                     /translation="GVSSTEIRHNPVASYLEQLGASDPEAAARLASTLQQDQNTMKTP
                     VTVLSDMLRAIRDAEHAQRISETTERAHRKADLLRGSLTSGNKKQTETGFTNPVNEQK
                     TCRDI"
BASE COUNT          140 a          115 c           97 g           59 t
ORIGIN      
        1 ggcgtaagca gcacagagat ccgccataac ccggtcgcca gctatctgga acaactgggg
       61 gcatcagacc ccgaagccgc cgcacgtctg gcatccacac ttcagcagga ccagaacacc
      121 atgaaaaccc ccgttaccgt gctttctgac atgttgcgcg ccatccgtga cgcagagcac
      181 gcacagagaa tcagtgaaac cactgagcgc gcccaccgca aagcagacct gctgcggggt
      241 agcctgacca gtggaaacaa aaaacagaca gaaacgggat tcacaaatcc cgtaaatgag
      301 caaaaaacgt gccgcgatat atgaagcgcg cacaaaacag gcaaaagcgg gatttaaaaa
      361 tcctgtaaac gattaattaa tcaacataag gaaaagcgac atgaaaattc a
//
LOCUS       DAHBHC010000429          407 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.434, whole genome shotgun sequence.
ACCESSION   DAHBHC010000429 DAHBHC010000000
VERSION     DAHBHC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.434"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>407)
                     /locus_tag="M2A35_005684"
     CDS             complement(<1..>407)
                     /locus_tag="M2A35_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7201797.1"
                     /translation="EKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESA
                     HFMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEHHGCVIHQSELDQS
                     NGRWICENTGMLTRDGLMFFSARGDEIPPPRSIT"
BASE COUNT           98 a          117 c           97 g           95 t
ORIGIN      
        1 gtgatggagc gcggcggcgg aatttcatca ccccgggcgc tgaaaaacat caggccgtca
       61 cgggtcaaca tgcccgtgtt ttcacagatc caccgcccgt tactctggtc aagctcagac
      121 tgatggatca cacagccatg atgctcacaa aggtagaaaa cactttctgg cttattcttc
      181 tcccacttaa gaccgaaagg cgaggcatca tcaccaaatt tcagatactg ctcctcccca
      241 cagtgcggac agggcacata aaaacgcatg aaatgtgccg actcgttagc ggctttttcg
      301 atctggcagg agcctttgat tttaggcgtc gagccgcgaa tggattttgg ccatacagag
      361 ccctcaatac gtttatcccc cagcagggtt ggcgacccct ctttttc
//
LOCUS       DAHBHC010000430          406 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.435, whole genome shotgun sequence.
ACCESSION   DAHBHC010000430 DAHBHC010000000
VERSION     DAHBHC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.435"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>406)
                     /locus_tag="M2A35_005685"
     CDS             complement(<1..>406)
                     /locus_tag="M2A35_005685"
                     /inference="COORDINATES: protein motif:HMM:NF017195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201798.1"
                     /translation="YGQYFGASYSAKPTEEVCLSRPELSNVSLSGFYKDDEGNQYVTI
                     HGCIYKATGVVICPDGTDSCTATWKPVTVDPAYSDKEDTEDKGDTEDKGDNGNTDGGD
                     NSGNLSGGGVSGGSSGGDNSGGSSGGSSSGGSS"
BASE COUNT          129 a          109 c           67 g          101 t
ORIGIN      
        1 ggatgaaccg ccggaactgc taccaccgga tgaaccgccg gaattatcac cacctgatga
       61 ccctcctgaa acgccaccac cggataaatt ccccgaatta tcaccaccgt cagtattacc
      121 gttatctcct ttatcttcag tatctccttt atcttcagta tcttctttat cagaataagc
      181 cggatcaaca gttacaggtt tccaggtagc ggtacaagaa tctgtaccat cgggacaaat
      241 aacaacaccc gttgccttat aaatgcatcc atgaatggtg acatattgat taccctcatc
      301 atccttataa aaacctgaca aagaaacatt actaagctca ggacgggata aacaaacctc
      361 ctcagtaggt ttagctgaat acgaagcacc aaaatattga ccgtaa
//
LOCUS       DAHBHC010000431          404 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.436, whole genome shotgun sequence.
ACCESSION   DAHBHC010000431 DAHBHC010000000
VERSION     DAHBHC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.436"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            72..362
                     /locus_tag="M2A35_005686"
     CDS             72..362
                     /locus_tag="M2A35_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCB7201799.1"
                     /translation="MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKL
                     RGLKDYCYKIKLRASGFRLVYQVIDDMLIIAVVAAGKHERSNVYHLASERMK"
BASE COUNT          124 a           83 c           84 g          113 t
ORIGIN      
        1 catgagctgg taaaactggt tactgaacgc agcagtcagc ctctgcatga tgtggacctg
       61 gacagttatt tatgacttat acagtaaaat tccgggatga tgcactgaaa gaatggctca
      121 gactggacaa gaccattcag cagcagttcg caaaaaaact gaaaaaatgc agtgaaaatc
      181 ctcatatagc ttctgcaaaa ctacgtggat taaaggacta ctgctataaa atcaaacttc
      241 gcgcatcagg ttttcgtctg gtttatcagg ttattgatga tatgttaatt attgcggtcg
      301 tcgcagcagg taaacatgaa cgcagcaacg tttatcatct cgccagtgaa cggatgaaat
      361 aatatcctgg tcagcactct ttcttacctg cttccatttt ttca
//
LOCUS       DAHBHC010000432          397 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.437, whole genome shotgun sequence.
ACCESSION   DAHBHC010000432 DAHBHC010000000
VERSION     DAHBHC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.437"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            46..>397
                     /locus_tag="M2A35_005687"
     CDS             46..>397
                     /locus_tag="M2A35_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCB7201800.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQESDRYS
                     GREDDPLVPRQLRQS"
BASE COUNT          111 a           98 c          117 g           71 t
ORIGIN      
        1 ctgacaatgt tgtaaaaaac gccgggccgg attataaaaa cggccatgag tcagaaatac
       61 ctcattcgca tcgctgagct ggaaaggctg ctctctgagc aggctgaagc cctccgtcag
      121 aaagaccagc aactgagtct ggttgaagag acggaggcct tcctgcgctc tgcactggca
      181 cgtgccgaag aaaagatcga agaagatgag cgagaaatag agcatctgcg ggctcagata
      241 gaaaaactgc gccggatgct gttcggaacc cgttctgaaa aactgcgtcg tgaggttgaa
      301 caggctgaag ccctgctgaa acaacgcgag caggaaagcg atcgttacag tgggcgtgag
      361 gatgacccac tggttccccg ccagttgcga cagtcgc
//
LOCUS       DAHBHC010000433          393 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.438, whole genome shotgun sequence.
ACCESSION   DAHBHC010000433 DAHBHC010000000
VERSION     DAHBHC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.438"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            6..212
                     /locus_tag="M2A35_005688"
     CDS             6..212
                     /locus_tag="M2A35_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCB7201801.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIKEDKRKAARGE"
     gene            212..>393
                     /locus_tag="M2A35_005689"
     CDS             212..>393
                     /locus_tag="M2A35_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7201802.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDI"
BASE COUNT          100 a           80 c          115 g           98 t
ORIGIN      
        1 atcagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg gggacgggat
       61 actggttact gcagctgctg gacaaagtct ctccgtccca gtgggtggca ataggtgtgc
      121 tgggaagtct gctgtttggc ctgctgacgt atctgacaaa tctttatttc aagattaaag
      181 aagataagcg taaggctgcg agaggtgaat aatgtcgccg tcattacgca aggctgttgc
      241 agctgctatt ggtggtgggg ctgttgccat agcgtctgtg ctcatcactg gtccaggtgg
      301 taacgatggt ctggaaggtg tcagctacat accatacaaa gatatcgttg gcgtatggac
      361 tgtatgtcac ggacacaccg gaaaagacat cat
//
LOCUS       DAHBHC010000434          393 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.439, whole genome shotgun sequence.
ACCESSION   DAHBHC010000434 DAHBHC010000000
VERSION     DAHBHC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.439"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            52..348
                     /locus_tag="M2A35_005690"
     CDS             52..348
                     /locus_tag="M2A35_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HCB7201803.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHGFIQISQKENVTVIIDG
                     GYVLGAFDCYVCAIKEVALLAARIEEADKKYGISYQRLKELSGTASSVHFFYAI"
BASE COUNT          128 a           66 c           84 g          115 t
ORIGIN      
        1 atttatgcca gcaatggcag ggattcgctc aaccttaaaa aggaaacagc aatgagcatt
       61 attgaatgtg aaattgaact tgatgttatt aacgattctc tcgccgaatt caaatgtggg
      121 aatacattac atggttttat tcagatttcc caaaaagaaa acgttacggt aattattgat
      181 ggcggttacg ttctgggagc atttgattgt tatgtgtgtg ccattaaaga agtagcatta
      241 ctcgcagctc gtattgaaga ggctgacaag aaatacggca tcagttatca acgactgaaa
      301 gaactctcag gcaccgcatc atcagttcat ttcttttatg caatctgaat atgagttgag
      361 gttaagaaac gatgagcacc gataaacaag ttt
//
LOCUS       DAHBHC010000435          393 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.440, whole genome shotgun sequence.
ACCESSION   DAHBHC010000435 DAHBHC010000000
VERSION     DAHBHC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.440"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>393
                     /locus_tag="M2A35_005691"
     CDS             <1..>393
                     /locus_tag="M2A35_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCB7201804.1"
                     /translation="LQGGPAAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVD
                     VNEWMRSLKRHERRALMRSLRDKQAKNSSDEMQNWSQSRKQPRPLPDNHELLAKEWRE
                     SAESLGLHIGEQQMLHLLRGGSLYVDGSI"
BASE COUNT          100 a          112 c          115 g           66 t
ORIGIN      
        1 cttcagggcg gccctgccgc cccttggact cgtggcaata actgtccccg tgtacaggaa
       61 acggacaaca acgggacaga acagccggaa gaacggccag caccgtggcc gcagcttcct
      121 gacggcgttg acgtgaatga atggatgcgc tcactgaaac ggcacgaacg tcgggcgctg
      181 atgcgttcgc ttcgcgacaa acaggcaaaa aacagcagtg atgaaatgca gaactggtca
      241 cagagccgca aacagccgcg gcctttgcct gataaccacg aattactcgc taaagaatgg
      301 cgggagtctg ccgaatctct cggcctgcat atcggtgaac aacagatgct gcacctgtta
      361 cggggcggca gtctgtacgt tgacggcagc atc
//
LOCUS       DAHBHC010000436          393 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.441, whole genome shotgun sequence.
ACCESSION   DAHBHC010000436 DAHBHC010000000
VERSION     DAHBHC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.441"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..262
                     /gene="traL"
                     /locus_tag="M2A35_005692"
     CDS             <1..262
                     /gene="traL"
                     /locus_tag="M2A35_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCB7201805.1"
                     /translation="TNQSRWFGLPLDELIPAAICIGWGITTSKYLFGIGAAVLVYFGI
                     KKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            284..>393
                     /locus_tag="M2A35_005693"
     CDS             284..>393
                     /locus_tag="M2A35_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCB7201806.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGV"
BASE COUNT           90 a           84 c          100 g          119 t
ORIGIN      
        1 gaccaaccag agccgctggt ttggcctgcc actggatgaa ctgatccccg cagcaatctg
       61 tattggctgg ggtatcacaa catcgaaata tctgttcggt attggtgcag cggttctggt
      121 ttatttcggg attaaaaaac tgaaaaaagg gcggggcagt tcctggttac gtgacctgat
      181 ttactggtat atgccaacag ccctgctgcg cggtattttt cataatgttc ccgattcgtg
      241 tttccggcag tggattaaat agaactgata ccaggattgt tatatggaac acggtgcccg
      301 tttaagtacc agtcgtgtaa tggccatcgc ctttatattt atgtcagtgc ttattgttct
      361 cagcctctct gttaacgtca ttcagggggt gaa
//
LOCUS       DAHBHC010000437          392 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.442, whole genome shotgun sequence.
ACCESSION   DAHBHC010000437 DAHBHC010000000
VERSION     DAHBHC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.442"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>392
                     /locus_tag="M2A35_005694"
     CDS             <1..>392
                     /locus_tag="M2A35_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201807.1"
                     /translation="PNIFDLTFTSQNSTLNTIKTMLSSAKTYDIETPKETLESFDLVD
                     HYYEVNSQKGVTLLTVYLDFQEVIQQMEVILSGSQSEQKPTDNNKSQGDVGVDPQITN
                     GGARESTVDELSKSWSSLKSSVGGITGK"
BASE COUNT          128 a           85 c           86 g           93 t
ORIGIN      
        1 cccgaatatt ttcgatctca cattcacaag ccagaacagc acgctgaaca caatcaaaac
       61 gatgctgtcc tctgcaaaaa catacgatat tgaaacgcca aaggaaactc tggagagttt
      121 cgaccttgtt gaccactatt acgaagttaa ctctcaaaaa ggggtgactc ttctgacggt
      181 ttatcttgat tttcaggagg tcattcagca aatggaggtt atcctctctg gatctcagtc
      241 agaacagaag ccaacggata acaataagtc acagggagat gtaggagttg atccgcaaat
      301 aaccaatggc ggagccaggg aatctactgt cgatgagcta agtaaatcat ggtcatcact
      361 gaagtcatct gttggcggaa tcactggaaa aa
//
LOCUS       DAHBHC010000438          388 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.443, whole genome shotgun sequence.
ACCESSION   DAHBHC010000438 DAHBHC010000000
VERSION     DAHBHC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.443"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>388
                     /locus_tag="M2A35_005695"
     CDS             <1..>388
                     /locus_tag="M2A35_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201808.1"
                     /translation="LREKREQVKESFRRNEDLTPLVRLAQGIVDAYEISLELPSQTWT
                     DSDGNRQHYVSCGLEAAEGFRRMPLSQIPAATPKARGSNDERKLTFSIETVVDDTPGE
                     VAFVHTPVSIAMYNDEIQVRVNNNIVP"
BASE COUNT          106 a           95 c          100 g           87 t
ORIGIN      
        1 agttaagaga aaaacgggag caggtaaagg agagctttcg ccgcaatgaa gatctgaccc
       61 cgcttgtacg ccttgcccag ggcattgttg atgcttatga aatctcgctg gagctgccat
      121 cacagacctg gacagatagc gacggtaatc gccagcatta cgtttcatgc ggactggaag
      181 cagccgaagg atttcgcaga atgcctttat cccagatccc tgccgctacc cccaaagcac
      241 ggggcagcaa tgatgagcga aaactgactt ttagtattga aacggtggtt gacgacacac
      301 ctggcgaagt cgcgttcgtg cacactcctg tttcgatcgc aatgtataac gatgaaatac
      361 aggttcgcgt taataataat atcgtgcc
//
LOCUS       DAHBHC010000439          388 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.444, whole genome shotgun sequence.
ACCESSION   DAHBHC010000439 DAHBHC010000000
VERSION     DAHBHC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.444"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..297)
                     /locus_tag="M2A35_005696"
     CDS             complement(<1..297)
                     /locus_tag="M2A35_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201809.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKG"
BASE COUNT           98 a           94 c           91 g          105 t
ORIGIN      
        1 gcctttacgt gaaataccat ttattttcac gttactggtc agtgcggctc catagccggt
       61 tgctggtgaa aaacagttat agacagttat cgccatatta ttggcatcat gaatcgccag
      121 cgccatcaga gccaccatct ggccgtcttt actgtctggt tcgaggtagg catcactgcc
      181 ataaatctgc tgaaaatagc taatcagggt gctgagtatc gtctgataat caggcgcact
      241 gatcccctcc gcggttacct ttgcagataa accgagagaa tcaaggttca gagccattac
      301 gcctccgatg taacagtcgt tattccatag agagtgtcga tttcagtgga aaacctgaca
      361 cgtcgggtcg tggtatccac cgtagtat
//
LOCUS       DAHBHC010000440          386 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.445, whole genome shotgun sequence.
ACCESSION   DAHBHC010000440 DAHBHC010000000
VERSION     DAHBHC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.445"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            143..>386
                     /locus_tag="M2A35_005697"
     CDS             143..>386
                     /locus_tag="M2A35_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCB7201810.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVG"
BASE COUNT          109 a           88 c           89 g          100 t
ORIGIN      
        1 agtacgcttg attccatccc tttatccatg cagcgacagt ttcctgattt aacaccgcgc
       61 catcttgacc atctgaaaac ccttattgct aagggggcaa atcagtgtgc gcgggcaggg
      121 gataaattac cggatttact tgatgaatat atcagagcaa caactgaata atatgatgag
      181 cgctgtcaca actgcattac agcccctgat aagggcattg ccggtgacgc cagttgaatg
      241 ggctgatcaa aattattatc tgcctaaaga atcttcatat ggtgagggcg aatggaaaac
      301 gctgccattc cagatcgcca tcatgaacag catggggaat gatcagatcc gcactgttaa
      361 tctgattaaa tctgcccgtg ttggct
//
LOCUS       DAHBHC010000441          383 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.446, whole genome shotgun sequence.
ACCESSION   DAHBHC010000441 DAHBHC010000000
VERSION     DAHBHC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.446"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..114
                     /locus_tag="M2A35_005698"
     CDS             <1..114
                     /locus_tag="M2A35_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCB7201811.1"
                     /translation="NVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            175..>383
                     /locus_tag="M2A35_005699"
     CDS             175..>383
                     /locus_tag="M2A35_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201812.1"
                     /translation="MGKGSSKGHTPREAKDNLKSSQMLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRA"
BASE COUNT           87 a           76 c          135 g           85 t
ORIGIN      
        1 aatgttctgc ctgttctgta cggtgaaatg cgcgtggggt cgcgggtggc ttctcaggag
       61 atcagcacgg cagacgaggg ggacggtggt caggttgtgg tgattggtcg ttgatgaaaa
      121 acgttttatg tgaaaccgcc tcagggcggt tttgtcgttt ctggagcgtg aggaatgggt
      181 aaaggcagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa atcatcccag
      241 atgctgagcg tgatagacgc catcagtgaa gggccgattg aaggtccggt ggacggatta
      301 aaaagtgtgc tgctgaacag tacgccagtg ctggacagtg aggggaatac caatatctcc
      361 ggtgtcacgg tggttttccg ggc
//
LOCUS       DAHBHC010000442          380 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.447, whole genome shotgun sequence.
ACCESSION   DAHBHC010000442 DAHBHC010000000
VERSION     DAHBHC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.447"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>380
                     /locus_tag="M2A35_005700"
     CDS             <1..>380
                     /locus_tag="M2A35_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCB7201813.1"
                     /translation="KTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFAL
                     LSSIIVSSAGNQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGKKDRMVAYTNDK
                     KYVRYPLVPLQSVPVQYRGLYQIVT"
BASE COUNT           83 a          105 c          101 g           91 t
ORIGIN      
        1 aaaacgtggg caacctcaac accggacgaa atccgcgcaa gcattaacca ggtgctgtct
       61 gatgcgtggg ccgcttccgg ttactctgtg gttccgcgtg atttgctgat cccgcctgag
      121 cagtttgctc tgttgtccag catcatcgtt tcatctgcgg gtaaccagtc cctgttgacg
      181 tatcttcaga ccaacaccat cagctatcac cagaacggtg ttccgctgaa tatccgcgcg
      241 gttaaatggc tgaaaggccg tggtgtgggg aaaaaggatc gcatggttgc gtacaccaac
      301 gataaaaaat acgtccgcta cccgctggtt ccgcttcaga gcgtgccggt gcagtatcgc
      361 ggtctgtatc agatcgtcac
//
LOCUS       DAHBHC010000443          379 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.448, whole genome shotgun sequence.
ACCESSION   DAHBHC010000443 DAHBHC010000000
VERSION     DAHBHC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.448"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>379
                     /locus_tag="M2A35_005701"
     CDS             <1..>379
                     /locus_tag="M2A35_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201814.1"
                     /translation="RFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDA
                     AGSARAASTSAGQAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKT
                     SETNAAASQKSAATSASTATTKAS"
BASE COUNT           97 a          115 c          113 g           54 t
ORIGIN      
        1 cgttttgaac tgatggtgga agaggtggcg cgtaacgcgt ccgcggtggc acagaacacg
       61 gcagccgcga agaagtcagc cagcgatgcc ggcacatcag cccgtgaggc ggcaacccat
      121 gcgactgatg ctgcaggctc agcacgtgca gccagcacat cagccggaca ggccgcttcg
      181 tcggctcagt cagcgtcttc cagcgcagga acggcatcaa caaaggctac tgaagcatca
      241 aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg ctaccagtgc tggtgcggcg
      301 aaaacgtcag aaacgaatgc tgcagcgtca caaaaatctg cagccacttc tgcatccacc
      361 gcgaccacga aagcgtcag
//
LOCUS       DAHBHC010000444          379 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.449, whole genome shotgun sequence.
ACCESSION   DAHBHC010000444 DAHBHC010000000
VERSION     DAHBHC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.449"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>379
                     /locus_tag="M2A35_005702"
     CDS             <1..>379
                     /locus_tag="M2A35_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCB7201815.1"
                     /translation="LTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHET"
BASE COUNT           95 a          105 c          122 g           57 t
ORIGIN      
        1 ctgactaacg agaacggcag ccacagcgac ttcagctacg atgcgctgga ccggttggta
       61 cagcagggcg gctttgacgg gcggacgcaa cgttatcatt atgacctgac cggaaaactc
      121 acgcagagtg aagatgaggg gcttgtcacc ctctggtact acgatgaatc ggaccgtatc
      181 actcaccgca cggtgaacgg cgaaccggca gagcagtggc agtatgatga ccacggctgg
      241 ctgacagaca tcagccacct gagcgaaggc caccgtgtcg ctgtccacta cggctacgac
      301 gataagggcc gcctgaccgg cgaacggcag acggtggaga acccggagac gggggaactg
      361 ctgtggcagc atgagacaa
//
LOCUS       DAHBHC010000445          376 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.450, whole genome shotgun sequence.
ACCESSION   DAHBHC010000445 DAHBHC010000000
VERSION     DAHBHC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.450"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>376
                     /locus_tag="M2A35_005703"
     CDS             <1..>376
                     /locus_tag="M2A35_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCB7201816.1"
                     /translation="WPVLSADIHGRVVRVLDGDTIEVMDSRKAVRIRLVNIDAPEKKQ
                     DYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYE
                     QYNTDPVLPVLQNEARQQKRGL"
BASE COUNT          100 a           77 c          110 g           89 t
ORIGIN      
        1 catggccggt attgtctgca gatattcatg gacgggttgt tcgtgtactg gacggtgata
       61 ccatcgaggt aatggactca cggaaggcgg tgcggatcag gctggtaaat attgatgcac
      121 cggaaaagaa acaggattat ggccggtggt caacagacat gatgaaatca ctggtcgcag
      181 gaaaaacggt taccgtcaca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
      241 tttatgcacc agacggaatg aatattaatc agtttatggt tcgggccggg gctgcctggg
      301 tatatgagca gtacaacaca gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac
      361 aaaaacgtgg gctctg
//
LOCUS       DAHBHC010000446          376 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.451, whole genome shotgun sequence.
ACCESSION   DAHBHC010000446 DAHBHC010000000
VERSION     DAHBHC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.451"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>376)
                     /locus_tag="M2A35_005704"
     CDS             complement(<1..>376)
                     /locus_tag="M2A35_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCB7201817.1"
                     /translation="LGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPA
                     VCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAE
                     LLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT           85 a          106 c          104 g           81 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtgtag
      361 ccttcacgac cgaggc
//
LOCUS       DAHBHC010000447          374 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.452, whole genome shotgun sequence.
ACCESSION   DAHBHC010000447 DAHBHC010000000
VERSION     DAHBHC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.452"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            49..>374
                     /locus_tag="M2A35_005705"
     CDS             49..>374
                     /locus_tag="M2A35_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCB7201818.1"
                     /translation="MTRPLIDVAAATRGLISAFDIDSAVGVQLDILGLWIGRSRVVSQ
                     PISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYLSDETYRVILKAKIAINNWDGRNDS
                     LPAILDA"
BASE COUNT           87 a           84 c           99 g          104 t
ORIGIN      
        1 acgaactacc acgccaccaa acctaaattt cttgcgcatg ttgatctgat gacccggcca
       61 cttattgatg ttgcggctgc caccagaggg ctgattagtg catttgatat tgactctgcg
      121 gttggtgtgc aacttgacat ccttggattg tggatcggac gtagccgtgt tgtcagccag
      181 cctatctcag gtgtctattt cagctgggat accgacgggc ttggatatga tcagggggta
      241 tggcagggac catacgatcc tgattcagga tacatgtatc tcagcgatga aacttatcgt
      301 gtcattctta aagcgaagat tgcgattaat aactgggacg gacggaatga ttcgcttccg
      361 gcaattcttg acgc
//
LOCUS       DAHBHC010000448          371 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.453, whole genome shotgun sequence.
ACCESSION   DAHBHC010000448 DAHBHC010000000
VERSION     DAHBHC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.453"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>371
                     /locus_tag="M2A35_005706"
     CDS             <1..>371
                     /locus_tag="M2A35_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCB7201819.1"
                     /translation="WVSNEQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVV
                     SEWATLPESGKKNKVYLKEVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSS
                     ENSGESSDQPENDLPVVKPDAA"
BASE COUNT          129 a           70 c           86 g           86 t
ORIGIN      
        1 tgggtgagca acgagcaact gtccgaattg accgggatat tgccgcacaa gtgttctgct
       61 gcaaaaagtg ttctggtaaa gcgtgggatt ttgattcaga gcgggcggaa tatcggtatt
      121 aataatgtgg tcagtgaatg ggcaacatta cccgaatcag gtaagaaaaa taaagtttac
      181 ctgaaagagg taaatttacc tgaatcaggt aagaaaagtt tacccaaatc aggtaaaggc
      241 gtttacccga atcaggtaaa cacaaaagac aaactaacaa aagacaatat aaaacctttt
      301 tcgtccgaga attctggcga atcctctgac caaccagaaa acgatcttcc tgtggtgaaa
      361 ccagatgctg c
//
LOCUS       DAHBHC010000449          369 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.454, whole genome shotgun sequence.
ACCESSION   DAHBHC010000449 DAHBHC010000000
VERSION     DAHBHC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.454"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            22..260
                     /locus_tag="M2A35_005707"
                     /pseudo
     CDS             22..260
                     /locus_tag="M2A35_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216630.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
BASE COUNT           76 a           76 c          108 g          109 t
ORIGIN      
        1 tcttcgtctg gagaatcacc tgtgacggta aaactgcgcc tggctgtggc tgcactcctg
       61 ctgtttctgg tggtgatggt ggatttcacc agcagaatca tgtcggtgct ggcggatggg
      121 gggctggtct gcggcattgt ggtattgctg tggccgatga tgaaaaaaca gaatgaataa
      181 ttcttgactt ttttgtttac tgtttattaa aaacacttct gcatggtgaa tccccctgtg
      241 cggaggggcg actggtgtag gtagcattta ttatgttata ggcaagccga cgcgggttca
      301 gtgacaccgg ctgaattcac cgggaggcac ccggcaccat gctttgccac aaaagtgtta
      361 tttctgttt
//
LOCUS       DAHBHC010000450          365 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.455, whole genome shotgun sequence.
ACCESSION   DAHBHC010000450 DAHBHC010000000
VERSION     DAHBHC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.455"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            56..>365
                     /locus_tag="M2A35_005708"
     CDS             56..>365
                     /locus_tag="M2A35_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCB7201820.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     R"
BASE COUNT          100 a           90 c           85 g           90 t
ORIGIN      
        1 ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc
       61 actgactgac gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga
      121 cggggctggc atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta
      181 tcgtattctg ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc
      241 cgaagctcgt acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg
      301 cgaacagaaa agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc
      361 acgac
//
LOCUS       DAHBHC010000451          363 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.456, whole genome shotgun sequence.
ACCESSION   DAHBHC010000451 DAHBHC010000000
VERSION     DAHBHC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.456"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..273
                     /locus_tag="M2A35_005709"
     CDS             <1..273
                     /locus_tag="M2A35_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201821.1"
                     /translation="TNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAE
                     DEAPPGIGEPDDEPQEPSGGNPLSNQPTQDSAGGRRHRKWSLRWFK"
BASE COUNT          103 a           97 c          104 g           59 t
ORIGIN      
        1 accaacgcaa tcagtacagc gctgggtgat ggtctgatga cactgaaagc cgctatgacc
       61 gatttgcgcg aaaattctga cgtaaccggc atcggggcat ccattaccga cgaggacatc
      121 gagaatgccg aagacgaagc gccgcccggc atcggcgaac ctgatgacga accgcaggaa
      181 ccgtcaggcg gaaatccgct atcgaaccag cctacgcagg atagcgcggg cggtcggaga
      241 catcgtaaat ggtcgctacg atggttcaaa tgacagtatc acggaaatta ttgaggcgct
      301 ggaacgctac agtgaaatca taaccccctg ggcgacaaag gtcgcggaaa actttaccgc
      361 cga
//
LOCUS       DAHBHC010000452          363 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.457, whole genome shotgun sequence.
ACCESSION   DAHBHC010000452 DAHBHC010000000
VERSION     DAHBHC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.457"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>363
                     /locus_tag="M2A35_005710"
     CDS             <1..>363
                     /locus_tag="M2A35_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HCB7201822.1"
                     /translation="NGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQR
                     QIEISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMI
                     RVNALQQNSKAKILSQPS"
BASE COUNT          119 a           68 c           73 g          103 t
ORIGIN      
        1 gaaacggcaa cctgtttgat aacagtgtaa agatttccgc agatccacgt cttaatgcag
       61 ttgttgtaaa agatcgtgaa ataactatgg acatatatcg gcaattaatt tctgagcttg
      121 atattgagca aaggcaaatt gagatttctg tttcaattat tgatgttgat gcaaatgatc
      181 ttcagcaact gggagtaaac tggtccggta cattaaatgc agggcaaggt tctattgcct
      241 ttaacagctc aacggcgcaa gctaatctca gttcttctgt gataagcaac gccaataatt
      301 tcatgatcag agtgaatgcg ttacaacaga actcgaaagc aaaaatactc tctcagccat
      361 caa
//
LOCUS       DAHBHC010000453          361 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.458, whole genome shotgun sequence.
ACCESSION   DAHBHC010000453 DAHBHC010000000
VERSION     DAHBHC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.458"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
BASE COUNT           93 a          103 c          123 g           42 t
ORIGIN      
        1 gcggcctttt gccgggatgc cgggagcgca ggccacggcg cgacagaggc ggcgcgaccg
       61 gaacggtcgc tgcaagccag ggtttcgcgt cagggatgga agcccgccag ggcggagaca
      121 gcttcgctgg ctccgttcac gacagcccgc cccgccaggg gacgcccgga aaagctataa
      181 acaggagagg aacggaaaga ggaagaagaa gaagaacagt aacaggcaca gtaaccacag
      241 aaatataaca tcaggaaaaa acattcctga cgcatcacaa acgggaaggg taacgggcga
      301 cgcgtcagcg tggcccgctt cccttccggc aggcgtcagc ctgccagggg ttttcgcgcg
      361 g
//
LOCUS       DAHBHC010000454          358 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.459, whole genome shotgun sequence.
ACCESSION   DAHBHC010000454 DAHBHC010000000
VERSION     DAHBHC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.459"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            206..>358
                     /locus_tag="M2A35_005711"
     CDS             206..>358
                     /locus_tag="M2A35_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCB7201823.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEH"
BASE COUNT           93 a          102 c           82 g           81 t
ORIGIN      
        1 ctgccagtaa agttgtcgcg cacgctggcg cacaaaagcg tcctgaatca ttgctccccc
       61 tgagtaatta caggaagatt acccgcgcgc gaaaccgttc tccttaaccc cctgttctga
      121 ctgttttctt acaacaaaag ccctttgtat cagcctgtta cgctttgcca tcatgactga
      181 agaaccagtc agaggggcaa aaactatggc taatgaaaaa aagacatccc gcaaaaagtt
      241 tcgcgtggct gtctccggat caactgttga tggccgcgaa atcagcccgg tacatctgcg
      301 tgaagccgcc gagaacttca acccggatgt ttacgctgcc cgcgtgaacg ttgagcac
//
LOCUS       DAHBHC010000455          356 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.460, whole genome shotgun sequence.
ACCESSION   DAHBHC010000455 DAHBHC010000000
VERSION     DAHBHC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.460"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>356
                     /locus_tag="M2A35_005712"
     CDS             <1..>356
                     /locus_tag="M2A35_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCB7201824.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAHBHC010000456          352 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.461, whole genome shotgun sequence.
ACCESSION   DAHBHC010000456 DAHBHC010000000
VERSION     DAHBHC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.461"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..125
                     /locus_tag="M2A35_005713"
     CDS             <1..125
                     /locus_tag="M2A35_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCB7201825.1"
                     /translation="LDEVTIKKMASRDFIQLCVVAVNFLSGADSGGKNEQAMEA"
     gene            134..289
                     /locus_tag="M2A35_005714"
     CDS             134..289
                     /locus_tag="M2A35_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCB7201826.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     SADNEDS"
BASE COUNT           78 a           82 c          108 g           84 t
ORIGIN      
        1 gactggatga agtgacgatc aaaaaaatgg catcccgtga ctttattcag ttatgtgtgg
       61 ttgccgtaaa ttttttgagc ggtgcggact ctggcgggaa gaacgaacag gcgatggaag
      121 cctgatcacg gttgtgtgct ttgagcacat agaagacttt gtggcagata ttgccgttat
      181 ttttaactgg tcgcccgccg aaatcttcat gatgacgccc ggcgaagtgg ttagctggcg
      241 tgagcgggcg gcacttcgca gcgggagtgc agacaatgaa gactcttgat atccgggtcg
      301 ctttcagcgc cgttgacagg ctgacccggc ctgccgaaaa cgcccgccgc ct
//
LOCUS       DAHBHC010000457          351 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.462, whole genome shotgun sequence.
ACCESSION   DAHBHC010000457 DAHBHC010000000
VERSION     DAHBHC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.462"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..107)
                     /locus_tag="M2A35_005715"
     CDS             complement(<1..107)
                     /locus_tag="M2A35_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCB7201827.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRS"
     gene            complement(123..>351)
                     /locus_tag="M2A35_005716"
     CDS             complement(123..>351)
                     /locus_tag="M2A35_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HCB7201828.1"
                     /translation="VNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPV
                     SILLALISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          115 a           64 c           58 g          114 t
ORIGIN      
        1 atagatctaa gcaccggatt gttaataggt gtattattca agcttactcg ttgaggatga
       61 atataactaa ttggatcata tataatcttt cttagggcaa gattcataac gctctcatat
      121 gtctacttaa ttttcaacct gactaaatta gttagaatgg ccctatactt ccataacagc
      181 cagcaagtcg ctacggatat taatgcaagt aagatagaaa ccggcatagc cttatcataa
      241 gcaaaaacag gttcgctaat ttcatatgtt ggtgcttgct caataatgtc tcttcgtttt
      301 gacaatacaa cagaaatatt ttcatattgt acgcttgcag agctattaac a
//
LOCUS       DAHBHC010000458          351 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.463, whole genome shotgun sequence.
ACCESSION   DAHBHC010000458 DAHBHC010000000
VERSION     DAHBHC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.463"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..155)
                     /locus_tag="M2A35_005717"
     CDS             complement(<1..155)
                     /locus_tag="M2A35_005717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201829.1"
                     /translation="MSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGLSAGMV
                     RPRWQKE"
     gene            complement(145..>351)
                     /locus_tag="M2A35_005718"
     CDS             complement(145..>351)
                     /locus_tag="M2A35_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201830.1"
                     /translation="GQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVDPYT
                     AYGAGFVQAHCELMPFDGGTPVEQ"
BASE COUNT           79 a          115 c           81 g           76 t
ORIGIN      
        1 tgctctttct gccagcgcgg acgaaccatc ccggcagaca gaccggaaac gttacgcatc
       61 cactggctta acagcctgtc gagcgcttcg tcataatccg gatcgccact ggttggtatt
      121 aaccatccgc gctctgtact ggtgttattg ctcaaccgga gttcccccat caaacggcat
      181 caactcacaa tgcgcctgaa cgaatccggc cccataagct gtatacgggt cgacgaaggt
      241 cacacgataa tcacggccct gatacgtcac gatatcggca tcacggccag tctgtccctg
      301 cgtcagtcgc tcagtcgtca cgatcagaat tgcaccgctg attacctgcc c
//
LOCUS       DAHBHC010000459          344 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.464, whole genome shotgun sequence.
ACCESSION   DAHBHC010000459 DAHBHC010000000
VERSION     DAHBHC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.464"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="M2A35_005719"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="M2A35_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCB7201831.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAHBHC010000460          341 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.465, whole genome shotgun sequence.
ACCESSION   DAHBHC010000460 DAHBHC010000000
VERSION     DAHBHC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.465"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>341)
                     /locus_tag="M2A35_005720"
     CDS             complement(<1..>341)
                     /locus_tag="M2A35_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCB7201832.1"
                     /translation="QCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMI
                     GFNGTSHADESNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGK
                     YANADALVQDAR"
BASE COUNT           65 a           93 c          100 g           83 t
ORIGIN      
        1 cgcgcgtcct gtaccagtgc atcagcgttc gcatacttac ccgcgtgtgc cacggtgttg
       61 tccatgttgc gggaggtcag cgtcacgtca ttcataacgc gttcgctggc gtcggttctg
      121 atgtgctcca gccagcccac gttaacgtcc tgaagcagct tgttggtgct gaagttggac
      181 tcatccgcgt gagacgtgcc gttgaaaccg atcatgatgc gatcaagtgc cacctgtcgg
      241 gcaatctgtg cgctgatgcg ggactgaaaa tcagggtgtg ccgcccaggc atcaagctgc
      301 ggatatgaaa taaacgtgtc gtagttcacc tgttcgcact g
//
LOCUS       DAHBHC010000461          338 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.466, whole genome shotgun sequence.
ACCESSION   DAHBHC010000461 DAHBHC010000000
VERSION     DAHBHC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.466"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>338
                     /locus_tag="M2A35_005721"
     CDS             <1..>338
                     /locus_tag="M2A35_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCB7201833.1"
                     /translation="SEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDT
                     PITRTITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIK
                     GKTTSQYLASV"
BASE COUNT           81 a           91 c          105 g           61 t
ORIGIN      
        1 agcgaaggga ataccaacat cgccggcgtc acggtggtgt tccgggcagg tgagcaggag
       61 cagacaccgc cggagggttt tgaatcctcc ggatccgaga cggtgctggg tacggaagtg
      121 aaatatgaca cgccgatcac ccggaccatc acgtctgcaa acatcgaccg tctgcgcttt
      181 accttcggtg tgcaggctct ggtggaaacc acttcaaagg gggaccggaa tccgtcggaa
      241 gtccgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc
      301 attaagggca aaaccacctc gcagtatctg gcctcggt
//
LOCUS       DAHBHC010000462          337 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.467, whole genome shotgun sequence.
ACCESSION   DAHBHC010000462 DAHBHC010000000
VERSION     DAHBHC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.467"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..164)
                     /locus_tag="M2A35_005722"
     CDS             complement(<1..164)
                     /locus_tag="M2A35_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201834.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHIL"
     gene            complement(164..>337)
                     /locus_tag="M2A35_005723"
     CDS             complement(164..>337)
                     /locus_tag="M2A35_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCB7201835.1"
                     /translation="GDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPD
                     DVDPLAELLKARD"
BASE COUNT           75 a          113 c           76 g           73 t
ORIGIN      
        1 aggatgtgct gcgcacgggg ttcactgaaa taaatccccc gctcttcgcc gtacttcaga
       61 tcatcaagaa aacgctggca ggccaggcgg acaaattcgc cagcaacaat ttctcctgca
      121 acaacacgtt cggcgtagcg gatcccgtca gccacttttg ccatcagtct ctcgctttta
      181 aaagctccgc cagcggatca acatcatccg gtccggcggt atttactttc gcccggcttg
      241 ccggtgacat accaaactct gcaagcatcg cccggatccg cttccaggca tccgccttca
      301 tcgcagcagc cgggtgtgcc ttaatcagca catcacc
//
LOCUS       DAHBHC010000463          336 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.468, whole genome shotgun sequence.
ACCESSION   DAHBHC010000463 DAHBHC010000000
VERSION     DAHBHC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.468"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..>336
                     /locus_tag="M2A35_005724"
     CDS             <1..>336
                     /locus_tag="M2A35_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCB7201836.1"
                     /translation="AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREAL
                     HWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLG
                     LEPKNTAVR"
BASE COUNT           75 a           80 c          115 g           66 t
ORIGIN      
        1 ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt
       61 cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga
      121 tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga
      181 cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga
      241 gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat
      301 gttgggactt gaaccgaaga acacggcggt gcggag
//
LOCUS       DAHBHC010000464          330 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.469, whole genome shotgun sequence.
ACCESSION   DAHBHC010000464 DAHBHC010000000
VERSION     DAHBHC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.469"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..135
                     /locus_tag="M2A35_005725"
     CDS             <1..135
                     /locus_tag="M2A35_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HCB7201837.1"
                     /translation="ISATHCCECGDPIDERRRLAVQGCRTCASCQEDLELISKQRGSK
                     "
BASE COUNT          121 a           57 c           77 g           75 t
ORIGIN      
        1 atatctgcca ctcattgttg tgagtgtggc gatcccatag atgagcgaag acgcctggcc
       61 gttcagggtt gtcggacttg tgcaagttgc caggaggatc tggaacttat cagtaaacag
      121 agaggttcga agtgagcgaa attaactaga agccaaagat aaaatcatcg ctgagcagga
      181 gaaaatcgct aacggagaaa agacagtaag tcagtatatg aaaaccgcat gatatcatca
      241 gataaaaatc gatcgtaaag cgaaatatta ataccagaat aaacgagtcg aggtaaatta
      301 tattacctcg ataaattaac taaaacttgc
//
LOCUS       DAHBHC010000465          330 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.470, whole genome shotgun sequence.
ACCESSION   DAHBHC010000465 DAHBHC010000000
VERSION     DAHBHC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.470"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..>330)
                     /locus_tag="M2A35_005726"
     CDS             complement(<1..>330)
                     /locus_tag="M2A35_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCB7201838.1"
                     /translation="HQIKIGEWIHADFKRVRNYAFHKRFFKLLQLGFDYWTPVGGAIT
                     PRERKLVSGFVDYLCESVGREHTPALSEAAEQYLNTVATRRTRDTALLKSFDAFREWV
                     TIQAGFY"
BASE COUNT           78 a           86 c           81 g           85 t
ORIGIN      
        1 tgtaaaatcc ggcctgaatg gttacccact cgcggaaagc gtcaaacgac tttagcaatg
       61 ccgtatcccg ggttctgcgt gtcgcaactg tattcagata ttgctctgcg gcttcgctca
      121 gagctggcgt atgttcccgg cctactgatt cgcacagata atcaacgaaa ccggacacca
      181 gttttcgttc gcgaggcgtg atcgcccctc cgactggagt ccagtaatcg aatcccagtt
      241 gcaggagttt gaaaaaacgc ttgtggaatg cgtagttacg aacgcgctta aaatcagcgt
      301 gtatccactc gcctattttg atttgatgca
//
LOCUS       DAHBHC010000466          330 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.471, whole genome shotgun sequence.
ACCESSION   DAHBHC010000466 DAHBHC010000000
VERSION     DAHBHC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.471"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(84..>330)
                     /locus_tag="M2A35_005727"
     rRNA            complement(84..>330)
                     /locus_tag="M2A35_005727"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           70 a           98 c           71 g           91 t
ORIGIN      
        1 ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc
       61 cgccaaaaca gcttcggcgt tgtaaggtta agcctcacgg ttcattagta ccggttagct
      121 caacgcatcg ctgcgcttac acacccggcc tatcaacgtc gtcgtcttca acgttccttc
      181 aggactctca aggagtcagg gagaactcat ctcggggcaa gtttcgtgct tagatgcttt
      241 cagcacttat ctcttccgca tttagctacc gggcagtgcc attggcatga caacccgaac
      301 accagtgatg cgtccactcc ggtcctctcg
//
LOCUS       DAHBHC010000467          329 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.denovo.472, whole genome shotgun sequence.
ACCESSION   DAHBHC010000467 DAHBHC010000000
VERSION     DAHBHC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.denovo.472"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..116
                     /locus_tag="M2A35_005728"
     CDS             <1..116
                     /locus_tag="M2A35_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCB7201839.1"
                     /translation="KGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            113..>329
                     /locus_tag="M2A35_005729"
     CDS             113..>329
                     /locus_tag="M2A35_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCB7201840.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAISYKEQRDKNAREL
                     KLANATIADMQQRQRDVAVLDAKYSREL"
BASE COUNT           84 a           77 c           95 g           73 t
ORIGIN      
        1 tcaaaggcgc atgtgatcag ctacggcgct ggacatacgc tggcggtaag caatggaaag
       61 ggctgatgac tcgccgtgag attgagcgtg aagtctgttt gtgggggcaa caatgagcag
      121 agtaaccgcg attatctacg ttctggtcat ctgcctcatc gtctgcctgt catgggctgt
      181 taatcattac cgtgataacg ccatctccta caaagagcag cgtgacaaaa acgccagaga
      241 gttgaagctg gcgaacgcca ccatcgctga catgcagcag cgtcagcgtg atgttgctgt
      301 gctcgatgca aagtactcga gagaattag
//
LOCUS       DAHBHC010000468        18586 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.guided.096, whole genome shotgun sequence.
ACCESSION   DAHBHC010000468 DAHBHC010000000
VERSION     DAHBHC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.guided.096"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="M2A35_005730"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="M2A35_005730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCB7201841.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="M2A35_005731"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="M2A35_005731"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCB7201842.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNISYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="M2A35_005732"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="M2A35_005732"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCB7201843.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="M2A35_005733"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="M2A35_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCB7201844.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDSE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="M2A35_005734"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="M2A35_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCB7201845.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="M2A35_005735"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="M2A35_005735"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCB7201846.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="M2A35_005736"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="M2A35_005736"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCB7201847.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="M2A35_005737"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="M2A35_005737"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCB7201848.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="M2A35_005738"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="M2A35_005738"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCB7201849.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="M2A35_005739"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="M2A35_005739"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCB7201850.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12901..14034
                     /locus_tag="M2A35_005740"
     CDS             12901..14034
                     /locus_tag="M2A35_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCB7201851.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="M2A35_005741"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="M2A35_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCB7201852.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="M2A35_005742"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="M2A35_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCB7201853.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="M2A35_005743"
     CDS             complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="M2A35_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCB7201854.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="M2A35_005744"
     CDS             complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="M2A35_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCB7201855.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15586..16152)
                     /gene="efp"
                     /locus_tag="M2A35_005745"
     CDS             complement(15586..16152)
                     /gene="efp"
                     /locus_tag="M2A35_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCB7201856.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16194..17222
                     /gene="epmB"
                     /locus_tag="M2A35_005746"
     CDS             16194..17222
                     /gene="epmB"
                     /locus_tag="M2A35_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCB7201857.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLTREIGGEPSKTP
                     LDLQLRQQ"
     gene            17612..18481
                     /locus_tag="M2A35_005747"
     CDS             17612..18481
                     /locus_tag="M2A35_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCB7201858.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLERAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4677 a         4843 c         4764 g         4302 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatca
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aaaataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggccgcttta aactcggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgc caggccacca
     3901 gccagacgtt acaggttcgg caggcgctga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgacagtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtacctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccactgg ttgccattct ggcgtcagcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggag attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc accgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agaaggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgccc cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccataca
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacacc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgct actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataacac
     8041 gtgcccactg aactgcactg cgcaacattg agcaggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttaacgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttgcc aatatttggc tctacgccaa aggtaaacag caattgtagc agcgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgcgact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactccag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcgtgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacttgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggttctaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gcaatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg
    12841 tatcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaacacgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg gcaataaaat tgcactggca gcacaccccg taaaagcgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacagggg caaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatagtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtaacag tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgttt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatagc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt agtgactatc gcagaaaaaa caaacggcac
    15121 gacatacctg tcgtaccgtt attgcttatt gctattgctg cgctttcgtt gctgcgccag
    15181 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
    15241 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
    15301 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
    15361 tcgccaaaaa tgacggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
    15421 gcgagagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca
    15481 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
    15541 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
    15601 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
    15661 aaccttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
    15721 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
    15781 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
    15841 agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
    15901 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
    15961 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
    16021 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
    16081 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
    16141 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
    16201 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
    16261 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
    16321 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
    16381 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
    16441 ttgtcgtcgc gtccggattc tccaccgacc cgctggaaga acagcacagc gtagtgcctg
    16501 gtttgttgca taaataccac aacagggcgc ttttgctggt caaaggcggc tgcgcggtaa
    16561 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
    16621 actggcagac tgctcttgag tatgttgctg cgcatccgga actggacgag atgattttct
    16681 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
    16741 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
    16801 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
    16861 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
    16921 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
    16981 acgcacaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgta atgccgtatt
    17041 acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
    17101 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg ccgaaactga
    17161 cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
    17221 aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc aggcctacat
    17281 tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc gtttacgccg
    17341 catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg gaaatttaat
    17401 ctttgcacgt tgcataacct ttaattattc atatgcattg ctgttgttac tttttatata
    17461 gctaacacca ataattaacc tttcttttct gtttatatag agtgagcacg gctatttatg
    17521 ttattttaac aactattact accaagccca tttatctggc ttataaatta aaattaatta
    17581 tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg gtgttaatac
    17641 cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta aagaacctcg
    17701 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
    17761 ggaaagtcgt atgcatcaaa agcataaact ggatgctgct gcacgccttc agtatgagca
    17821 agctcgcgat aaagtcatta aaaaaatggc agaaaacatc ccagaaattc tggttgatga
    17881 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
    17941 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
    18001 tgagttgcag attgaaatgc tggagcgagc aatcattcat gcaattaata atgcggaaat
    18061 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat atgacgttga
    18121 ctgccaggaa aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
    18181 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
    18241 atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
    18301 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
    18361 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
    18421 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
    18481 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
    18541 cgttggtcat gccggatgcg gcgtaaacgc cttatccggc ctacaa
//
LOCUS       DAHBHC010000469        10376 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.guided.126, whole genome shotgun sequence.
ACCESSION   DAHBHC010000469 DAHBHC010000000
VERSION     DAHBHC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.guided.126"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            complement(<1..1254)
                     /locus_tag="M2A35_005748"
     CDS             complement(<1..1254)
                     /locus_tag="M2A35_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCB7201859.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTL"
     gene            complement(1258..1818)
                     /locus_tag="M2A35_005749"
     CDS             complement(1258..1818)
                     /locus_tag="M2A35_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCB7201860.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            complement(1994..2344)
                     /locus_tag="M2A35_005750"
     CDS             complement(1994..2344)
                     /locus_tag="M2A35_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCB7201861.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2547..3560)
                     /gene="intI1"
                     /locus_tag="M2A35_005751"
     CDS             complement(2547..3560)
                     /gene="intI1"
                     /locus_tag="M2A35_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCB7201862.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3718..4191
                     /locus_tag="M2A35_005752"
     CDS             3718..4191
                     /locus_tag="M2A35_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HCB7201863.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            4322..5110
                     /locus_tag="M2A35_005753"
     CDS             4322..5110
                     /locus_tag="M2A35_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HCB7201864.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            5312..6016
                     /locus_tag="M2A35_005754"
     CDS             5312..6016
                     /locus_tag="M2A35_005754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCB7201865.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            6069..6824
                     /locus_tag="M2A35_005755"
                     /pseudo
     CDS             6069..6824
                     /locus_tag="M2A35_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(7006..7182)
                     /locus_tag="M2A35_005756"
     CDS             complement(7006..7182)
                     /locus_tag="M2A35_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCB7201866.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            7512..8327
                     /gene="sul2"
                     /locus_tag="M2A35_005757"
     CDS             7512..8327
                     /gene="sul2"
                     /locus_tag="M2A35_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCB7201867.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            8414..8716
                     /locus_tag="M2A35_005758"
     CDS             8414..8716
                     /locus_tag="M2A35_005758"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201868.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(8892..10037)
                     /locus_tag="M2A35_005759"
                     /pseudo
     CDS             complement(8892..10037)
                     /locus_tag="M2A35_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
BASE COUNT         2131 a         2972 c         2974 g         2299 t
ORIGIN      
        1 cagggtctgc acggctgcca gcacgccttg cgcggccggc gcggcgcgca gttccagcac
       61 ctccagcaag gccggcgtgt aacggcgcag ggtggcgaag ttctcgccga ccaggtgcag
      121 gtggtcgaag ccttccggcc gggccagcag ctcggcctcg ctgacgctct cggtgaactc
      181 gtcccaggga atcaccgcct cgatggcggc ataggggtcg ctgccgcttt ccttcgcttc
      241 cagcagcgcc tggccgatcc tggagtacag gcgcaccttg tcgttgatcg ccttgccctg
      301 cttctggaac tgctgctgat gcttgtgctt cgcgccgctg aacagcttga ccaggatgcg
      361 gtcatgcaga tcgaccagct catcgatcac ggtcgcggtg ctctccagca ccacggcggc
      421 cagggtcgcg tagcggcgct gcggctcgaa cttaccgagg tctttgggcg tcatctgccc
      481 accctcgcgg gccagcttga gcaggcggtt ctggtggatg tgccggccca ggccttcggg
      541 caagtccacc aactgaaatg tcttcagccg ctcgatgtgt tccagcatgt gccgagagtt
      601 gggtttcagc ggtgcctggc gcagccaggt caaccaggtg atgctgctgc cggccttgag
      661 cttcaacagc tcgtccagct tggcccgatg cgagtccgtg agtggttcga ccagggcgcg
      721 gtagacccgc cgattggctc gcgcaatggc ttccgagcag gcccggtcaa tcacgctcag
      781 cgccggcagg atgcgtcgtt tctgccgtag gctctccagg gcctgaccgg ccagcagcaa
      841 gcctttgtcg gtctgctggg ccagctcggt tagctcgcgc accagggcgc ggaagtcgga
      901 caggccgaac ggggccagtt gcaggtaggt gcgcagttcc tgggcatgct cgcgacgggt
      961 cacgtcgcgc tcgccgtact ttgcccagct cgccggctcg gcctggactt gcttcgccac
     1021 ccacaggatg accggctcgg gcagctcgct gtcggttccc agcgcgtaac cggggtagcg
     1081 cagcaggcag agctgcacgg cgaagccgag gcggttggcg tcgccgcgtc gctggcggat
     1141 cagcgacagg tcggagtcgt tgaaggtgta gtagcggatc aggtcatcct ggctttccgg
     1201 cagcgcaagc agggtgtccc gctccgtggc cgagaggatc aagcgacgcg gcatgtgtca
     1261 gtcgtccgtg cggaggtact ggtagagggt ttcccggctg atgttgaact cgcgggcaag
     1321 ctgcgccttg ggctcgccgg ccgtcgctcg ctgccgcagg gtagcagcct gctcatcgga
     1381 cagggctttc ttgcggcccc ggtacgcgcc acgctgcttg gccaaggcga tgccctcacg
     1441 ctgccgctcg cggatcaggg cgcgctcgaa ctcagcgaag gcccccatca ccgacagcat
     1501 caggttggcc atcggcgagt cctcgccagt gaacaccagg ccctccttca ggaactcgat
     1561 gcgcacgccg cgctgagtca gcttctgtac caagcgacgc aggtcatcga ggttgcgggc
     1621 cagccgatcc atgctgtgca ccaccactgt atcgccttcg cggacgaagc tcagcagcgc
     1681 ttcgagctgg gggcgctggg tgtccttgcc cgatgccttg tcggtgaaca ccttgctcac
     1741 ctgggtttgt tccagctggc gttccgggtt ctggtcaaag ctgctgaccc ggacgtagcc
     1801 gatgcggtgc ccctgcacga tgtctccttg gttgaaggcg gcttaagtgc actttctgtt
     1861 ccgttgtgcc tcaaagccca tttctgtcag gctgaaatct ataaccttcg cgggcatgtg
     1921 tcaaaaaatg ggaaagcaga ctctattctg acgaagcggc gcggccctgc ctgacatcaa
     1981 gttagggtat gcctcaacct gacggcggcg aatcacaagc gtccggtttg acgctggttt
     2041 cggtcgcagt gctggcccgc gcctggaagc gctgatagca ctccagcccg cagaaatgct
     2101 ccacgtactc ggccccttcc ggcgtgaagg cggcatcgag cgggatttcc ttgcagcaca
     2161 cgcagcaact ggtgctcggt tcattggcgt tcatggtggt gttcctccat tggttgacga
     2221 agccgacgaa ggccgccgcc ggcatcggcc tggcgaacag gaaaccctgc accgtgtcgc
     2281 aacccgcctg ccgcaaccac gcaaggcagt cgggtgtttc cacaccctcg gctaccacct
     2341 ccattgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag
     2401 cggaggggtt ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg
     2461 aggactttcc gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga
     2521 atccgccagg attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca
     2581 agcggtgagc gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta
     2641 gagacgtcgg aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc
     2701 aaggccgtcg cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct
     2761 tgttctacgg cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc
     2821 acgacaccgc tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc
     2881 caggaatgcc cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg
     2941 cggccctcgg cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg
     3001 ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc
     3061 acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg
     3121 atccgcatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa
     3181 ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga
     3241 ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac
     3301 agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc
     3361 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg
     3421 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa actgtaatgc
     3481 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     3541 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg
     3601 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     3661 gatgttacgc agcagggcag tcgccctaaa acaaagttag ccattaaggg agttaaattg
     3721 aaaatatcat tgatttctgc agtgtcagaa aatggcgtaa tcggtagtgg tcctgatatc
     3781 ccgtggtcag taaaaggtga gcaactactc tttaaagcgc tcacatataa tcaatggctc
     3841 cttgtcggaa gaaaaacatt tgactctatg ggtgttcttc caaatcgcaa atatgcagta
     3901 gtgtcaaaga acggaatttc aagctcaaat gaaaacgtcc tagtttttcc ttcaatagaa
     3961 aatgctttga aagagctatc aaaagttaca gatcatgtat atgtctctgg cgggggtcaa
     4021 atctataata gccttattga aaaagcagat ataattcatt tgtctactgt tcacgttgaa
     4081 gtcgaaggtg atatcaaatt ccctataatg cctgagaatt tcaatttggt ttttgaacag
     4141 ttttttatgt ctaatataaa ttatacatac cagatttgga aaaaaggcta acaatgcgtt
     4201 gcagcaccag tcgcttcgct ccttggacag cttttaagtc gcgtctttgt ggttttgctg
     4261 cgcaaaagta ttccacaaag ccgcaactta aaagctgccg ctgaacttaa cgttaggcat
     4321 catgggtgaa tttttccctg cacaagtttt caagcagctg tcccacgctc gcgcggtgat
     4381 cgagcgccat ctggctgcga cactggacac aatccacctg ttcggatctg cgatcgatgg
     4441 agggctgaag ccggacagcg acatagactt gctcgtgacc gtcagcgccg cacctaacga
     4501 ttcgctccgg caggcgctaa tgctcgattt gctgaaagtc tcatcaccgc caggcgatgg
     4561 cggaacatgg cgaccgctgg agctaactgt tgtcgctcga agcgaagtag tgccttggcg
     4621 ctatccggcg cggcgtgagc ttcagttcgg tgagtggctc cgccacgaca tcctttccgg
     4681 aacgttcgag cctgccgttc tggatcacga tcttgcgatt ttgctgacca aggcgaggca
     4741 acacagcctt gcgcttctag gcccatccgc agccacgttt ttcgagccgg tgccgaagga
     4801 gcatttctcc aaggcgcttt tcgacactat tgcccagtgg aatgcagagt cggattggaa
     4861 gggtgacgag cggaacgtcg ttcttgctct tgctcgcatt tggtacagcg cttcaactgg
     4921 tctcattgct cctaaggacg ttgctgccgc atgggtatcg gagcgtttgc ctgccgagca
     4981 tcggcccctc atctgcaagg cacgcgcggc gtacctgggt agcgaggacg acgacctagc
     5041 aatgcgcgtc gaagagacgg ccgcgttcgt tcgatatgcc aaagcaacga ttgagagaat
     5101 cttgcgttga gcggcatgtg cgaaaagtgc atcgacccgc gccgagggca tctgatgcct
     5161 aactcggcgt tcaagcggac gggctgcgcc cgccgctcaa ctatgcgtta gatgcactaa
     5221 gcacataatt gctcacagcc aaactatcgg cactgttgca aatagtcggt ggtgataaac
     5281 ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc ggcattttca
     5341 gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca gttaccgtga
     5401 gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga tttaccgctg
     5461 ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc gtaacccttc
     5521 cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc gctgggcgta
     5581 tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct cctcccgtcg
     5641 taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga agaagtggca
     5701 gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc ttgctctgct
     5761 caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt accggaacaa
     5821 cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc tgggatttaa
     5881 atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg cactacgcaa
     5941 aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc tggtaagcag
     6001 agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc taactttgca
     6061 acagtgccgt taaaagcaac aaaaatgact tcgttgatgc tgaagctatc tgtgaagcag
     6121 catcacgtcc atctatgcgt ttcgtgcagc ccagaaccga atctcagcag gcaatgcgag
     6181 ctctgcatcg tgtccgtgaa tccctggttc aggataaggt gaaaacaact aatcagatgc
     6241 atgcttttct gctggaattt ggtatcagcg ttccgcgagg tgctgccgtt attagtcgac
     6301 tgagtaccct tcttgaggac agtagtttgc ctctttatct cagccagtta ctgctgaaat
     6361 tacaacagca ttatcactat cttgttgagc agattaaaga tctggaatct cagttgaaac
     6421 gaaagttgga cgaagatgag gttggacagc gcttgctgag tattccctgc gttggaacgc
     6481 tgactgccag tactatttca actgagattg gcgacgggaa gcagtacgcc agcagccgtg
     6541 actttgcggc ggcaacaggg ctggtacccc gacagtacag cacgggaggt cggacgacat
     6601 tgttagggat tagcaagcgg ggcaacaaaa agatccgaac tttgttggtt cagtgtgcca
     6661 gggtattcat acaaaaactg gaacaccagt ctggcaagtt ggccgactgg gtcagggagt
     6721 tgttgtgtcg gaaaagcaac tttgtcgtca cctgtgctct ggcaaacaag ctggccagaa
     6781 tagcctgggc actgacggcg cgacagcaaa cttacgaagc ataaaggcag aaatacacca
     6841 gtttaaacaa tcattcatct ggttttgcga atactgatat tgatgatact aacggcccac
     6901 cggcctgttg aggaacctgt aaaacggaaa ggctcattga agccgtatat tttctggagg
     6961 ttcatcaggc gcggaactca tcgaggcgcg ggaataaaat cccattcaga cgccggatag
     7021 attcaagcaa gccaacttgt cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat
     7081 tccgttttct gaggcgaccc ccacatagag accttcccgg tcattcactt tgtagagttt
     7141 gtccctgggc ttgagattgc gcagcttggt atcggtcagc atgtaccttg tccttcttcg
     7201 tctccgatac ccactctatt gtaaacaaga catttttatc ttttatattc aatggcttat
     7261 tttcctgcta attggtaata ccatgaaaaa taccatgctc agaaaaggct taacaatatt
     7321 ttgaaaaatt gcctactgag cgctgccgca cagctccata ggccgctttc ctggctttgc
     7381 ttccagatgt atgctattct gctcctgcag ctaatggatc accgcaaaca ggttactcgc
     7441 ctggggattc cctttcgacc cgagcatccg tatgagactc atgctcgatt attattatta
     7501 tagaagcccc catgaataaa tcgctcatca ttttcggcat cgtcaacata acctcggaca
     7561 gtttctccga tggaggccgg tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc
     7621 tgatggccga gggggcagat gtgatcgacc tcggtccggc atccagcaat cccgacgccg
     7681 cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg
     7741 cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt
     7801 cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc
     7861 aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag
     7921 atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc
     7981 gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca
     8041 tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg
     8101 aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc
     8161 gcgcgctcac aggccgtggt ccgggggatg tcggggccgc gacactcgct gcagagcttg
     8221 ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg
     8281 ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg
     8341 atatttctct atattcgcgg ttcagcaggc atgtcccctt tgagggcgac ccgacgacag
     8401 gataatcgac cttatggtgc gcaaatattt cggcacagac ggtattcgtg gcaaagccaa
     8461 cgaaggcgcg atgacggcgg aaaccgcctt gcgcgtcggc atggcggctg gccgtgtctt
     8521 tcgtcgcggt gaccaccgcc atcgtgtcgt gatcggcaag gatacgcgcc tgtcgggcta
     8581 tatgcttgaa cccgcgctca cagccggttt cacctcgatg ggcatggacg tattcctttt
     8641 tggcccgctg ccgacaacgt ataggaagaa taaacgccct tttcacccaa gtccaacagc
     8701 tttggaccgc agttgactct ttcgacaccc ctgcgatgca acccaatccg gctgacgggg
     8761 agccagcaac gctgaaaatt taccctcctc tttcccacta gcggctcctt ttccgacaac
     8821 cagcacggcg gatccctgcc gcggcgctgt gaacgcagca ttttgattgg tatcgttggc
     8881 cttcaggctc gtcagtcaaa cagacccagg agcagctcag ccggtggcgc ccggctttcg
     8941 ggtaacgccc tggtcccgct ggtttcggct ttgtgcttca ggtgatcaag gatctgcttg
     9001 atcactatag ggtcttcaat gcaggcgatg actttcatgg cgccgccgca gccgctgcag
     9061 gtctcgatgt cgatattgaa aacacgcttg agccgttgcg cccatgtcat cgacgctcgc
     9121 cgttgtgctg gtgttgccgg ttcatcagcc accctgacct tgttgcccct gccccgtttt
     9181 gccggcgtga ccaacgcccg gtgccgactg ttgggtgcga acaccccgtg gaagcgggtt
     9241 aggttgactc tgggcttcgg taccagggcg gccagccttg caatgaaatc caatggttcg
     9301 aaaatgacgt gcgtggtgcc gtcccggtac ggcgtcttga gctggtagcg cacgttgccg
     9361 cctcgtgtta acgacagccg cttctcggat accgccgggc ggctgatgta ccggcacagc
     9421 cgttcgagct tcttgcgttc atcggccctg gccgccacgc cggcgtgcag gctggacccg
     9481 gctaccttgc caatcccgtc accgaacgga tcaccactgg tcggcagagt ttgcaaagtg
     9541 aacacctttc gccccgcctg tgaaccgaca gcgatacggt aagtgatcga gtgccccagc
     9601 aggggtgtca tcgggtcgtc atccaccgca tccgaggcca gatagctgtt ttcgacatcc
     9661 cgttccagca ggccttgccg ttccagatag cgacccaccc ggtgggcgat ggtgtgcgtc
     9721 agctgggtga gctctggggt ggtcggcgcc ttgacccagc ggaaacgcgc tgagccgtgg
     9781 gattgctcga catacacacc gtcgagaaac agcatgtgga agtgaacatt cagattgagc
     9841 gccgatccaa aacgctggat cagggtgacc gcgcccgtct tggccacttg gtgggtatgg
     9901 cccgctttct tgaccaggtg cgtggcaatg acgcggtaaa cgatgcccag cacccacccc
     9961 atgatctcgg gccggctggc aaacaggaaa cgcagctgaa acgggaagct caacacccac
    10021 tgacgcatgg gttgttcgtc ctggacggcg aacccggcaa agctcctgta acattgcttc
    10081 ttcctgtttt gttagcgtga ctgtcacgct agcgccttcc tctttgagtc gcgcgcggcg
    10141 ccgggcctgg cgaatacggg cttgttcacg ctgtcggtca ttcatatttt tcctccacgt
    10201 gactgtcacg ttgaattaaa ctgggcatta cccctgcggg gccgttctgc cgtgctgcgc
    10261 atcgagccgc tacgcgtctc gccccttcgg gcggcggcca ctaatgcctc cggcctgcgg
    10321 cctccgcgct tcgcttgtga cgatatctga atcgttatat actgtgtata tacgta
//
LOCUS       DAHBHC010000470         8610 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.guided.139, whole genome shotgun sequence.
ACCESSION   DAHBHC010000470 DAHBHC010000000
VERSION     DAHBHC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.guided.139"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            503..871
                     /locus_tag="M2A35_005760"
     CDS             503..871
                     /locus_tag="M2A35_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201869.1"
                     /translation="MSEDLKQNLIALLEEQFIRSDDKVVFDYVMQKKIKSQGYHLQRN
                     FSISISGGRKGFIDCLVTSSDGQQCAIEVDKKSPRNRSLMKLAQLPEGMSGFVLLRDG
                     KHPLRYSENGIDVIRATKFK"
     gene            983..2641
                     /locus_tag="M2A35_005761"
     CDS             983..2641
                     /locus_tag="M2A35_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCB7201870.1"
                     /translation="MQLTITPNFAQERALNMLRRDWKANDTFMVYSPTGSGKTGLAAF
                     IVAGFVSRGMRVLFCAPYTILIGQTANRFVEYGLPGDEIGYIWADHPNYDPDRKIQIA
                     SADTLIRRVFPENIDLLIIDEAHLRKKRILKDIERLRGKGVKVIGLSGTPFSPFLGKY
                     YDRLIKPTTIGELIQRGDLSKYEFYAPTKPDLKGVKTTSSLEYGRDYNETQLAEIMCG
                     STLVGDIVQNWLENGRDLPTIAFCVNVAHANYLTIQFNLAGVNAEVMTADTPVDERQT
                     IIHRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQALGRGLRTAPGKES
                     CLIFDHSGTVHRLGYPDSIEYDDLPGKSDGMEESARRAVEERAEKLPHECPQCHYMKP
                     AGVYVCPKCGHKPLRGEDVDTDTSRKLNKLGKNQHQSTKAEKQSWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEISPEVSNYIKHKLIRFAKGRQRTQRTI
                     EKLQATIPLSQERGERSEMPIGTEAWRIMQAKQQLQKNINSLSQ"
     gene            2643..3611
                     /locus_tag="M2A35_005762"
     CDS             2643..3611
                     /locus_tag="M2A35_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCB7201871.1"
                     /translation="MKTAAAAKGRWPEILEHFGLPPITGKNHFKGECPVCGARGKFRI
                     DDRDGAGTWICVCGSGDGMKLVTLTQAKPFNEICTEIDRLIGNDYQRVKIPVTSSATS
                     LRKRVLSKFSKLEALRGTSGASYLNSRGIFSLPAEAIRFNARQRHNGSVFQSLYSLAT
                     DDKGELCYLHQTLLDGDKKADIGSSAKRLKSLQEDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYNVNTWATINSGFMKKFRVPAGVLHLIIFADRDENSATGLAAACEC
                     AHANLMAKNDLQRVSVYWPDHDDFNNMLMNGDQVRELVFHKKKAVA"
     gene            3611..4468
                     /locus_tag="M2A35_005763"
     CDS             3611..4468
                     /locus_tag="M2A35_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCB7201872.1"
                     /translation="MRTDNNEHKALFTIPTAAYSSALANIKPLPEQRRITGHKQTDAY
                     LWVLEVIRLNEPAHLDAAEAALVKIKISPKEAQERYSRYLLANGGDPFQIAFGTIGMD
                     NPAQAIRIARENIKKATSVRATFGSYEAALEDVEAERVIKSSQKFIDDHLWGWTAAEK
                     TAGRIDGIRMNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFVYWDWLYSVRHTASKEQ
                     GDEYGYSEHHESVYDRERYLEKLLMTIKPVTRAEAVEVCRWFLASGKGEYMKDNGATV
                     ILNLVGECE"
     gene            4468..5298
                     /locus_tag="M2A35_005764"
     CDS             4468..5298
                     /locus_tag="M2A35_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCB7201873.1"
                     /translation="MKLEASLKYFSPQGMYIGDDVKETSPERLTGTDVMAAIGTTSNR
                     ERFGLAAFFGKAGISKTDEQMAVQALARHAMEIAPKNVRKAAGGEFGWCMLVLAQFAF
                     AEYSRSAATSVTCHSCSGTGLTPRKQVIRKVSYPWGKAPYWASRSRAVRPSDWEKWTE
                     VTEVVLVVCDVCRGKGVISARCRCGGKGEVLDRKATSERGVPVFKICERCSGNGFSTV
                     PSTTVYKVVLKRIPELHVRTWTRNWKPFLDSLVDICHQEERKADIAFQNVTSFGDDVN
                     "
     gene            complement(5413..6333)
                     /locus_tag="M2A35_005765"
     CDS             complement(5413..6333)
                     /locus_tag="M2A35_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCB7201874.1"
                     /translation="MIFLRTENGIEKLENWEEIISRPNFVTTIDKGVQQLEEIIGYYK
                     FKEEIHCGLTGCNQPHQMGYIVKTSSGIETNIGNKCGKNEFGVEFGENVLSFNKFMQL
                     ETNREIISTAKSKCEEWQKNIETIRNIKPTIDYLSSTIENSKNSNYSGRLGATEIRFL
                     TKSQTGLVTLSEVETDKNTKTILFAMNEHMRESGEAISEFYIGKVSFSHVLLPENNLR
                     DFFVALKEDLKKIQCIDLQTAPSPEISNVAQIASSIEERIKRLKKLKHEASKFLTKKN
                     LRPIANKIKYSSTADELEFHNFERFLNGLK"
     gene            6822..7781
                     /locus_tag="M2A35_005766"
     CDS             6822..7781
                     /locus_tag="M2A35_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit A"
                     /protein_id="HCB7201875.1"
                     /translation="MRHILLKLVLFFCVCLSSASYADEFTVDFSSQKSYVDSLNSIRS
                     AISTPLGNISQGGVSVSVINHVPGGNYISLNVRGLDPYSERFNHLRLIMERNNLYVAG
                     FINTETNTFYRFSDFSHISVPDVITVSMTTDSSYSSLQRIADLERTGMQIGRHSLVGS
                     YLDLMEFRGRSMTRASSRAMLRFVTVIAEALRFRQIQRGFRPALSEASPLYTMTAQDV
                     DLTLNWGRISNVLPEYRGEEGVRIGRISFNSLSAILGSVAVILNCHSTGSYSVRSVSQ
                     KQKTECQIVGDRAAIKVNNVLWEANTIAALLNRKPQDLTEPNQ"
     gene            7794..8057
                     /locus_tag="M2A35_005767"
     CDS             7794..8057
                     /locus_tag="M2A35_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2f subunit B"
                     /protein_id="HCB7201876.1"
                     /translation="MKKMIIAVLFGLFSANSMAADCAVGKIEFSKYNEDDTFTVKVSG
                     REYWTNRWNLQPLLQSAQLTGMTVTIISNTCSSGSGFAQVKFN"
BASE COUNT         2279 a         1820 c         2189 g         2322 t
ORIGIN      
        1 actgccaggc agacgaagag tggattcagc agcagttaca ggggctgcct ccgtcactga
       61 gacggaaggt cgccctgaaa tatgcggagg tatacgaaat cacttttgac gctgagcctg
      121 tttcattccg caaggagaac agagcaaggc acgaagcaaa cacaaggctc cgcttgtttg
      181 tgagaaatca gggcagagct ttacaggggt atacaactca gccgcccctg gcaggaacgc
      241 aatcgcgctc ctgattggta ccgggcttaa aggtgtccgg tggctgaatc ccaaatctca
      301 ttgcatttgt gtactagcta aggagtacat gcaaaattca gtgagaggag gggaggggga
      361 ggagtgctcg tgtgttagtg cgaagcactg gaacaggctt ttccaacaga cgggtacata
      421 ggttaggtag atctcgatct aaagggggat acccctgaaa aaacggccgt accataaagc
      481 tagtacaaga tggataaaaa gtatgagtga agacctgaag caaaatttaa tcgctctcct
      541 ggaagagcaa ttcattcgct ccgatgacaa agtcgttttc gattatgtga tgcagaaaaa
      601 aatcaagtct cagggatacc acctgcaacg caatttcagc atcagcatta gcggtggtcg
      661 taaagggttt attgattgcc tggttacatc atcagacggc cagcagtgtg ccattgaggt
      721 cgataagaag tctccccgca accgttcgtt gatgaagctg gctcagctac ctgaggggat
      781 gtcaggcttt gtcctgctca gggatggtaa gcaccctctt cgatatagcg agaacggaat
      841 tgacgttatt cgtgcgacga aatttaagtg agttgattcg gaaaggggct ggcagccttt
      901 ggggagacca ccagccatgt gaggaggaat ccatgaaaac cacatcacaa aattattatc
      961 tcatcactgc ggggacagca caatgcagct gacaatcacg ccgaattttg cacaggaacg
     1021 agcgttaaac atgttgcgcc gtgactggaa ggcaaacgac accttcatgg tgtactcgcc
     1081 aaccggtagc ggtaaaacgg gtttggccgc cttcatcgtt gccgggtttg tcagccgtgg
     1141 tatgcgcgtc ctgttctgtg caccgtacac catcctgatc ggtcagacgg ctaatcggtt
     1201 cgtggagtat ggattaccgg gggatgaaat cggttatatc tgggcggacc acccgaacta
     1261 cgatccggac cggaaaattc agattgccag cgccgacacg cttattcgtc gtgtttttcc
     1321 tgaaaatatt gatctgctga ttatcgacga agcacacctg cgtaaaaaac gcatcctgaa
     1381 ggatatcgaa cgtctgcgcg gtaaaggcgt aaaggtgatt ggcctgtcgg gtactccgtt
     1441 ttccccgttc ctgggcaaat actatgaccg actgattaag ccgaccacca tcggcgagtt
     1501 aatccagcgt ggcgatctga gtaaatacga attttacgcg ccaactaagc cggatctgaa
     1561 aggtgttaaa accacatctt cgcttgagta cggccgcgat tacaacgaaa cacagctggc
     1621 tgaaatcatg tgcggctcta cgctggtggg cgacatcgta cagaactggc tggagaatgg
     1681 tcgggatcta cctaccatcg ctttctgcgt caacgtagcc cacgccaatt acctgacaat
     1741 ccagtttaac ctcgcaggtg ttaacgctga ggtaatgacc gcagacactc cggtagatga
     1801 gcgccagacc atcattcacc gctttgaaac tggtgcaacg aaaatcatcg ttagtgtggg
     1861 cgttctggtg gccggctttg atagtgacgt tcgttgcatc atctacgcca ggccaacaaa
     1921 aagcgaaatt cgctggttgc aggcactcgg gcgtgggctg cgcaccgcac cgggtaaaga
     1981 gtcctgcctt atcttcgatc acagcggcac cgtgcaccgt ttgggttatc cggattcaat
     2041 cgagtacgac gatcttcccg gtaagtctga cggcatggag gaaagcgcgc gccgcgcagt
     2101 tgaggaacgg gccgaaaaac tgccacatga atgccctcaa tgccactaca tgaagccagc
     2161 tggcgtctat gtttgcccta aatgtggaca caagccgctg cgaggtgaag atgttgatac
     2221 tgacactagc cgcaaactta ataagctggg taaaaatcag catcagtcga cgaaggcaga
     2281 gaaacagtcc tggtggagtc agatcaaatt ttatcagcgc cagcgtgctt cgctggggcg
     2341 tccagtcagt gatggatggt gtgctcacac tttcagggag aaattcggtg aatggcctga
     2401 tgggttgagc agtttcccga tggaaataag cccagaagta agtaactaca tcaaacacaa
     2461 gctgattcgg tttgccaaag gccgtcagcg gacgcagaga actatagaaa aattgcaggc
     2521 aacaattcct ctgtctcagg aacgaggtga gcgaagcgag atgccaatag gcactgaggc
     2581 ttggcgcatc atgcaagcaa agcaacaact ccagaaaaat ataaacagtt tgagtcagta
     2641 agatgaaaac agcagctgca gcgaaaggcc gctggcctga aatattagag cacttcggct
     2701 tgccgccgat aacaggaaaa aatcacttca agggtgaatg cccggtatgc ggtgcacgtg
     2761 gcaagttccg aattgatgac cgcgacggtg caggaacgtg gatctgtgta tgtggtagtg
     2821 gcgatggtat gaaacttgtc accctgacac aggcgaagcc atttaacgag atttgtaccg
     2881 aaatagaccg cctgatcggt aatgattacc aacgggttaa aatcccggta accagcagcg
     2941 ccaccagctt acgcaaacgg gtattgagca agttttcaaa actggaggca ctgcgtggta
     3001 catccggcgc atcgtatctt aattctcgtg gaatattcag tcttcctgct gaggcgatcc
     3061 ggttcaatgc caggcagaga cacaacggga gtgtgttcca gtctctttat tcacttgcta
     3121 cggacgataa aggggagttg tgctatctgc accagactct gcttgatggt gataaaaaag
     3181 cagatatcgg tagcagtgca aagcgcctca aatccctgca ggaagataac tatttggatc
     3241 acgctcgttc tgtagctatc cgcatgtttc ctgttgctag cactctgggt atcgccgaag
     3301 gcatcgaaac agcgctgtca gcgcaccaga tttataacgt gaacacctgg gcaaccatta
     3361 acagcggctt tatgaaaaag ttccgcgtac cagctggtgt tctgcacctg attatttttg
     3421 ccgaccgtga cgagaacagc gccaccgggc tggctgcggc ttgcgaatgt gctcatgcca
     3481 atctgatggc aaagaatgac ctgcagcgcg tgagcgtgta ctggccggat cacgatgatt
     3541 tcaacaatat gctcatgaac ggtgatcagg ttcgagagct ggttttccat aagaaaaagg
     3601 cggttgcgta atgcgtactg ataacaacga acataaagca ctattcacca tcccgacggc
     3661 agcgtacagt tccgccctcg caaacatcaa gcccctgcca gagcaacgga gaatcaccgg
     3721 gcataagcag actgatgctt atctttgggt gctggaggtt atccgtctga acgaacccgc
     3781 acatctggat gctgctgagg ctgcgctggt gaaaattaaa atttccccaa aagaggccca
     3841 ggaacgctat tcgcgttatc tgctggcgaa tggtggtgat cctttccaga ttgctttcgg
     3901 taccatcggc atggataacc cggcacaggc aatcaggatc gcccgggaga atatcaaaaa
     3961 agcaacatca gtcagggcta cgtttggtag ctatgaagca gcactcgaag atgtggaagc
     4021 cgagcgagtg atcaagtctt cccagaaatt tatcgacgat catctctggg gctggactgc
     4081 agccgagaag acagcgggca gaattgatgg catccgtatg aatgaaattg atgatcagcg
     4141 tcgtgcatat gttgatggct atcgtgatgt actgccagag cctcatacat tgtcagacgt
     4201 agttcgtgag tttgtttact gggactggct ctacagtgtt cgccacactg cgtctaaaga
     4261 acagggcgat gagtatggtt actctgagca tcacgaatcg gtatatgacc gcgagcgcta
     4321 ccttgaaaaa ttgctgatga ccatcaaacc ggtgacacgg gctgaagccg tggaggtgtg
     4381 tcgttggttt ttggcaagcg gaaagggcga atatatgaaa gacaatggcg cgacggttat
     4441 tcttaatctg gttggggagt gtgaataatg aagcttgagg catcgctaaa atacttcagt
     4501 cctcagggaa tgtatatcgg cgacgatgtg aaagaaacct ctccggaacg tcttacaggc
     4561 accgatgtta tggcggctat tggtaccacc agcaatcgtg agcggtttgg cctggcggcc
     4621 ttcttcggga aggccggtat cagcaagact gatgagcaga tggcagtcca ggcgctggcg
     4681 cgtcacgcga tggaaattgc accgaagaat gtgcgtaaag cagctggtgg tgaatttggc
     4741 tggtgtatgc tggtactggc gcagtttgct tttgctgagt attcccgatc ggcggccacc
     4801 agcgtaacat gccatagttg cagtggtacc ggactaacac cccgtaagca ggtcattcgt
     4861 aaggtttcat acccatgggg taaagcacca tattgggcca gtcgctcccg tgctgttcga
     4921 ccgtcagatt gggagaaatg gacagaggta acggaagttg tactagtcgt ttgcgatgta
     4981 tgtagaggga agggagtgat aagtgccagg tgtcgttgtg gtggaaaggg agaggtactg
     5041 gaccgcaaag ccacaagcga gcgtggtgtg ccagtgttta aaatctgcga acgttgcagc
     5101 ggaaatggat tttccacggt accgtctacc acagtatata aagttgtact gaagcggata
     5161 cccgagctac atgtcaggac atggacgcgt aattggaagc cgtttcttga ttctctggtt
     5221 gacatttgcc accaggaaga gcgtaaggcc gatattgctt ttcagaatgt gacaagtttt
     5281 ggtgatgatg tgaactaagc ttagggtttt tgcgacattg cacttgattt tgtccgaatc
     5341 tgtcatgtat tcttttaagc atgcggaata atgcgtaaat gattttgata gaagcccctt
     5401 tcggggcttt tatcatttca gcccgtttaa aaagcgttcg aaattatgga attcaagttc
     5461 atccgctgtc gatgagtatt ttattttgtt tgcgatggga cgcagatttt ttttagttag
     5521 gaatttactg gcttcatgtt tcagtttttt taatcgcttg attcgttctt cgattgaact
     5581 ggcaatttgg gcaacattag aaatttctgg gcttggggct gtttgtagat caatacactg
     5641 aatttttttt agatcttctt tgagcgcaac aaaaaaatca cgaagattgt tttctggtaa
     5701 aagaacatga ctaaatgata cttttcctat gtagaattca cttattgcct ctccagactc
     5761 gcgcatgtgt tcattcattg cgaacagaat tgttttggta ttcttatcag tttccacttc
     5821 agaaagggtt acaaggcctg tctggctctt ggtcaaaaaa cgaatttctg tagcaccaag
     5881 tcttccggag taattggaat ttttactatt ttcgatggta gaagatagat aatctattgt
     5941 gggtttgata tttcgtatcg tttcaatatt tttttgccat tcttcacatt tactttttgc
     6001 ggtgctaata atttcccgat tggtttcgag ttgcataaat ttattgaaac taagaacgtt
     6061 ttcaccaaat tccacaccaa attcgttttt accacattta tttccgatgt tggtttcaat
     6121 gccactagat gtttttacaa tataacccat ttgatgtggt tggttgcaac cagttagacc
     6181 acagtggatt tcttctttaa atttgtagta accaattatc tcctcaagtt gttgaactcc
     6241 tttgtctata gtggtgacaa agtttggtct ggatataatt tcctcccagt tttcaagttt
     6301 ctcgatgccg ttttcagttc tgagaaaaat catgaatcgc tccatttgaa agtggaccgt
     6361 ttattttatc ggcataaaaa tgagaacacc acaagttatt ttattaccga atacttggta
     6421 aagatgggta acttcgccac acagcttaaa cacgccgccg ggcggttttt ttatgtccgt
     6481 aaaccggtcg tggtgccagg ggggggcata gtcaatggac gctcctcttg accaggctgt
     6541 ggctgaagta aagtttatgg tgttctttat atgtgagaga ttctctattc tataaaaata
     6601 taaaccatat acccgtaatc ttaaataatt gatactattt tccggaagct atagtgcatc
     6661 attatctgct ttctgttcct gataaacagg gaagtaatac attgtgcagt catttcctgt
     6721 gaggggattt gtttgtaata aatttctgct aaaaacattc cggacttctt gcgaggtatt
     6781 attcctggcg ctgccggttc agactgatgg agtgttaata tatgcgacat atattattaa
     6841 agctggtgtt gtttttttgt gtttgcttgt cttcagcatc ttatgcagat gagtttactg
     6901 tggatttctc ttcgcaaaag agctatgttg attcattgaa tagtataagg tcggcaatat
     6961 ccactccact tggaaatata tctcagggtg gtgtttctgt ttcagtaatt aatcatgttc
     7021 caggcggaaa ctatatatca ttgaatgtta gaggccttga tccatatagc gagagattta
     7081 accacctccg tttaataatg gaacggaata acttatatgt tgcaggcttt attaatactg
     7141 aaacgaatac cttttacaga ttctccgatt tctcacatat ttcagtgcct gatgtgataa
     7201 ctgtttccat gacgacggac agcagttatt catcattaca gcgaatcgca gatctggaac
     7261 gtacagggat gcagattggg cgtcattcac tggttggttc atatctggat ttaatggagt
     7321 tcagaggacg ttccatgacc cgcgcatcat ccagagctat gctgcgtttt gtcacagtga
     7381 tagcagaagc tctgcgattc agacaaatac agcggggatt ccgaccggcg ctgtctgagg
     7441 catctccgct ttatacaatg acggctcagg atgttgacct taccctgaac tggggaagaa
     7501 taagtaatgt tcttccagag tacagaggag aggaaggggt aagaatcggt aggatatctt
     7561 ttaatagtct ttctgcgatt ctcggaagtg ttgcggtcat ccttaattgc cactcaaccg
     7621 gaagttattc agttcgttcc gtgagccaaa aacagaaaac agaatgccag attgttggag
     7681 acagggcggc cattaaagta aataatgttt tgtgggaagc gaatacaatc gctgctttat
     7741 taaatcgcaa gcctcaggat cttactgaac caaaccaata acagggggtg aatatgaaga
     7801 agatgattat tgcagtttta ttcggtctct tttctgctaa ttccatggcg gcggattgtg
     7861 ctgtaggaaa aattgagttt tccaagtata atgaggatga tacctttact gtgaaggtgt
     7921 caggaagaga atactggacg aacagatgga atttgcagcc attgttacaa agtgctcagc
     7981 tgacagggat gactgtaaca atcatatcta atacctgcag ttcaggctca ggctttgccc
     8041 aggtgaagtt taactgagaa tctacggttt atttatgcgc gtcttttgtt tctggacgca
     8101 gatattatta gtgttgtgga tgctgattaa atttggtcag tgttttcgtt aaagtcatat
     8161 aaatacaggg gcgttcacgc ccctttttgg tctgtagttg ggtgaagatc atcactttac
     8221 tttgctcaaa taactcagca cttgccggtt tacccttaag gcaataccta cgttccataa
     8281 aagaagcatc aattaggatg ctcgtataca ttgttatgtg ctttaaatgt taactgatgg
     8341 ggatgctgaa ataccggaca tgtactgctt gcgtgtccct cacgcttcga aaatcgggcg
     8401 gtatgtcaga atcgtggtga gtgtgaatat cgctctgtaa ttctgagtgt tttttcaaca
     8461 gatgaataag cagacattga actctgttta tttatcagta tgatttcttt ggttccgagg
     8521 gaagggtcaa ttatgtatcc gggcatctca ttcacacccg aggaaccagc gccgacttag
     8581 ctcagtaggt agagcaactg acttgtaatc
//
LOCUS       DAHBHC010000471         6474 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.guided.157, whole genome shotgun sequence.
ACCESSION   DAHBHC010000471 DAHBHC010000000
VERSION     DAHBHC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.guided.157"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..111
                     /gene="prfH"
                     /locus_tag="M2A35_005768"
     CDS             <1..111
                     /gene="prfH"
                     /locus_tag="M2A35_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCB7201877.1"
                     /translation="QQENSAVQKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(168..1625)
                     /gene="pepD"
                     /locus_tag="M2A35_005769"
     CDS             complement(168..1625)
                     /gene="pepD"
                     /locus_tag="M2A35_005769"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCB7201878.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1886..2344
                     /gene="gpt"
                     /locus_tag="M2A35_005770"
     CDS             1886..2344
                     /gene="gpt"
                     /locus_tag="M2A35_005770"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCB7201879.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2436..3680
                     /gene="frsA"
                     /locus_tag="M2A35_005771"
     CDS             2436..3680
                     /gene="frsA"
                     /locus_tag="M2A35_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCB7201880.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3738..4139
                     /gene="crl"
                     /locus_tag="M2A35_005772"
     CDS             3738..4139
                     /gene="crl"
                     /locus_tag="M2A35_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCB7201881.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4178..5233)
                     /gene="phoE"
                     /locus_tag="M2A35_005773"
     CDS             complement(4178..5233)
                     /gene="phoE"
                     /locus_tag="M2A35_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCB7201882.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            5521..>6474
                     /gene="proB"
                     /locus_tag="M2A35_005774"
     CDS             5521..>6474
                     /gene="proB"
                     /locus_tag="M2A35_005774"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCB7201883.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNL"
BASE COUNT         1565 a         1605 c         1654 g         1650 t
ORIGIN      
        1 caacaggaaa atagcgcggt gcagaaatcg cagcggcgaa tgttccatca ccagattgaa
       61 cgtggcaacc cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg
      121 aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga
      181 atttctttca gcagttcagt cagcagcgtc cagtaatgac ctacgctttc gatgtgaact
      241 tgctcatccg gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc
      301 ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggt
      361 gttttgttga acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg
      421 tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc
      481 agcgaatcca gcatgctcac gacgtagtct ttaccgctgt cgatcagtga acggatcaga
      541 cagtgaattt ctacgttatt atcagtcatg gtcaccacac cgacgttcag ggaggtttcg
      601 accacacctt ttgccacgtc tgagttgcgg atcacaccgt tcggggtggc gttcagcaga
      661 cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc
      721 agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag
      781 gtattcatca gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag
      841 gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc
      901 agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt acccaggcca
      961 acgtggattt ccccgccgga gtgaccccct ttcagacctt ttaaggttaa cttgaaggtt
     1021 tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc
     1081 cccgcacaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca
     1141 gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg
     1201 gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac tgccagtgca
     1261 gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg
     1321 ccatcaatat aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc
     1381 tgtggcacca tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg
     1441 gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc
     1501 tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga
     1561 atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca
     1621 gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc
     1681 agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     1741 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     1801 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     1861 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     1921 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     1981 tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat
     2041 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     2101 agtgctgaaa cgtgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     2161 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     2221 cttcgcaaaa ccggccggtc gcccgctggt tgatgattat gttgttgata tcccgcaaga
     2281 tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     2341 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     2401 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     2461 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     2521 acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc
     2581 gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa atcctcgacg
     2641 tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta tacgatactg
     2701 tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc
     2761 aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg
     2821 ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag
     2881 cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc
     2941 ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga
     3001 aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg
     3061 actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg
     3121 atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc
     3181 atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg
     3241 cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc
     3301 gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt
     3361 gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg
     3421 atgcttccga tgacgcgttg cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat
     3481 tgctgggacg tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca
     3541 gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga
     3601 tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc actggctgga
     3661 tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat
     3721 cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa
     3781 aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt
     3841 tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg
     3901 gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac cagtttggtc
     3961 tgttcgataa agcaggcgac tggaagagcg ttccggtaaa agacactgaa gtggttgaac
     4021 gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga
     4081 atctgaagct ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta acggcgtgag
     4141 tgaaatgtgc cggatgcatc acatccggca ataatcatta aaactgatag gtcatgccaa
     4201 ccgcgacaat atcatcatta ttaatattca atttgttatc gctatccagt tggttgattt
     4261 tataatcaac aaacgctgac atatttttgt tgaaataata tgtagccccg acatcgatat
     4321 aattgaccag atcttcatca ccgatacctt caatatcttt ccctttcgat aagacataac
     4381 ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg
     4441 tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa taaaaagttg
     4501 ccagataaat attattggca tcgtatttca gacctgttgc ccatgcttct gcacgcttgc
     4561 cagtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggcaccac
     4621 taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt
     4681 tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc
     4741 catcgataac gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg
     4801 tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa ttccgggaac atatcggtcc
     4861 aggcttccac gtcatacaac gcgcccaggt tacggccata gtcgaaagaa cccaaatctt
     4921 tatacttcaa tccggcaaaa gcgagacgcg ttttttgctg tgcagtatca ctctccgctt
     4981 tatttccggc aaactccgct tcccaacggc catagccagt cagttgatcg ttaatttgtg
     5041 tttcgccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg tcgttatcac
     5101 tcatataatg catggcttta actttgccat agacatccag tttattaccg tctttgttat
     5161 atatttctgc ggcctgtacg gatgcagatg ccacaatgcc catcaccact aatgccagag
     5221 tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc
     5281 ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac agattaataa
     5341 tattcttaaa atgtggtaat ttattaaatc tgtaataaaa gcgtaaacaa ctgccgctag
     5401 gcttgctgat cccgcgcaac aaaacgccat gctttgcttg cagatggttg gcaaccgacg
     5461 acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg gcagagaatc
     5521 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
     5581 cgccgtctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc
     5641 gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg
     5701 gggtacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcggc ggtagggcag
     5761 agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcag
     5821 atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc
     5881 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct
     5941 acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattctggcg
     6001 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgatccgcgc
     6061 agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg
     6121 attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa attgcaggcc
     6181 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
     6241 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
     6301 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta
     6361 gatgaagggg caactgccgc cattcttgaa cgcggcagct ccctgttgcc gaaaggcatt
     6421 aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctc
//
LOCUS       DAHBHC010000472         3675 bp    DNA     linear   BCT 29-APR-2022
DEFINITION  TPA_asm: Escherichia coli strain 476
            SAMN26797224-rid14326423.guided.205, whole genome shotgun sequence.
ACCESSION   DAHBHC010000472 DAHBHC010000000
VERSION     DAHBHC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN26797224
            Sequence Read Archive: SRR18965776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-APR-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001296369.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001296369.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/29/2022 18:49:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 79 of 186
            Pseudo Genes (incomplete)         :: 89 of 186
            Pseudo Genes (internal stop)      :: 45 of 186
            Pseudo Genes (multiple problems)  :: 22 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN26797224-rid14326423.guided.205"
                     /strain="476"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="United Kingdom: Scotland"
                     /collection_date="2017-07-05"
                     /collected_by="Scottish E.coliO157,STEC Reference
                     Laboratory"
     gene            <1..200
                     /locus_tag="M2A35_005775"
     CDS             <1..200
                     /locus_tag="M2A35_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCB7201884.1"
                     /translation="EAGARTRATAHERTPQQERQKAAREAERGREAWTLGQGMKKPVA
                     GCYGRLTRWKGGGDVVYMALL"
     gene            complement(232..882)
                     /gene="tetR(A)"
                     /locus_tag="M2A35_005776"
     CDS             complement(232..882)
                     /gene="tetR(A)"
                     /locus_tag="M2A35_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCB7201885.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            988..2187
                     /gene="tet(A)"
                     /locus_tag="M2A35_005777"
     CDS             988..2187
                     /gene="tet(A)"
                     /locus_tag="M2A35_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCB7201886.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATTPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2219..3103)
                     /locus_tag="M2A35_005778"
     CDS             complement(2219..3103)
                     /locus_tag="M2A35_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCB7201887.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3241..3648)
                     /locus_tag="M2A35_005779"
     CDS             complement(3241..3648)
                     /locus_tag="M2A35_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCB7201888.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          701 a         1152 c         1138 g          684 t
ORIGIN      
        1 acgaggccgg cgcgcggacc cgcgccacgg cccacgagcg gacgccgcag caggagcgcc
       61 agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga
      121 aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg
      181 tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc
      241 accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac
      301 aatcaccgcg agtccctgct cgaacgctgc gtccggatcg gcttcgtcga aggcgtctat
      361 cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc
      421 atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat
      481 cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc
      541 gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc
      601 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca
      661 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag
      721 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc
      781 caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc
      841 cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact
      901 gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac
      961 cagcggaggc tgctccggag gccagacgtg aaacccaaca gacccctgat cgtaattctg
     1021 agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc
     1081 ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg
     1141 tatgcgttga tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg
     1201 cggcggccgg tcttgctcgt ctcgctggcc ggcgctgctg tcgactacgc catcatggcg
     1261 acgacgcctt tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg
     1321 actggggcgg tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg
     1381 cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt
     1441 gggctgatgg gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc
     1501 ctcaatttcc tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg
     1561 ttacgccggg aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc
     1621 gtcgccgccc tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg
     1681 ctttgggtca ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg
     1741 cttgccgcat ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc
     1801 gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac
     1861 atcctgcttg ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct
     1921 tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa
     1981 cgtcaggggc agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga
     2041 cccctcctct tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg
     2101 attgcaggcg ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc
     2161 ggcgcagggc aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc
     2221 aaggcgactt ccggcaagct atacgcgccc taggagtgcg gttggaacgt tggcccagcc
     2281 agatactccc gatcacgagc aggacgccga tgatttgaag cgcactcagc gtctgatcca
     2341 agaacaacca tcctagcaac acggcggtcc ccgggctgag aaagcccagt aaggaaacaa
     2401 ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga
     2461 tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat
     2521 caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa
     2581 aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg
     2641 aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca
     2701 acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta
     2761 tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta
     2821 ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata
     2881 gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct
     2941 gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcatc gcgaccgtca
     3001 tcggtgagaa gttcggcagg tattgggtgg tgacaatgta ggtgctgccc caaatggcag
     3061 gtgctatcgc tgtgaacaat aaatcgggcg tgcgtaatga catgcgatta caagacctcc
     3121 gctacggcga tgggtttttg agagttgcag gcgccgcgat gaccgcaccc tgacttggac
     3181 tggccctgcc tcgcaccaat ccacctgcgg ctatggccgc gatcatgcga ggcccgaaaa
     3241 ttacacgacc tgtacacgtc cgccgaaccc ggaacgcaac agagcttcgt gggcatcccg
     3301 atccgggtcg tagcagcagt cctggaccaa acgcacgtcg tagtccgcat cactagccca
     3361 ggcgacgctt gaaagaacaa cgccggtggt gcttatcccg gccatgacaa gcgtgcttac
     3421 gccgcgcgtc cgaaggtcgg cgtcgagcgc cgtgccataa aagacgctgg ctcgcggaca
     3481 tgcatagaaa aggtcgcccc gttcgatcgc aagcccttcg acgggcagac cggtgcgaaa
     3541 tcgtccgctc gggagatacg gtgagatttg gcgattcgtc gccggcggcg catgttcata
     3601 ctcttcgccg agcgaaaagt tggggaacag cacgggccgg ccgcgcaagg cgtgctggca
     3661 gccgtgcaga ccctg
//
